ALFA: The new ALICE-FAIR software framework

Science.gov (United States)

Al-Turany, M.; Buncic, P.; Hristov, P.; Kollegger, T.; Kouzinopoulos, C.; Lebedev, A.; Lindenstruth, V.; Manafov, A.; Richter, M.; Rybalchenko, A.; Vande Vyvre, P.; Winckler, N.

2015-12-01

The commonalities between the ALICE and FAIR experiments and their computing requirements led to the development of large parts of a common software framework in an experiment independent way. The FairRoot project has already shown the feasibility of such an approach for the FAIR experiments and extending it beyond FAIR to experiments at other facilities[1, 2]. The ALFA framework is a joint development between ALICE Online- Offline (O2) and FairRoot teams. ALFA is designed as a flexible, elastic system, which balances reliability and ease of development with performance using multi-processing and multithreading. A message- based approach has been adopted; such an approach will support the use of the software on different hardware platforms, including heterogeneous systems. Each process in ALFA assumes limited communication and reliance on other processes. Such a design will add horizontal scaling (multiple processes) to vertical scaling provided by multiple threads to meet computing and throughput demands. ALFA does not dictate any application protocols. Potentially, any content-based processor or any source can change the application protocol. The framework supports different serialization standards for data exchange between different hardware and software languages.

Componentes ricos em plaquetas na reparação de afecções tendo-ligamentosas e osteo-articulares em animais Components rich in platelets used in wound healing tendon, ligaments and osteo-articular diseases of animals

Directory of Open Access Journals (Sweden)

Leandro Maia

2009-07-01

Full Text Available O plasma rico em plaquetas e o concentrado de plaquetas são fontes de diversos fatores de crescimento, com grande potencial terapêutico. Uma vez liberados dos grânulos alfa das plaquetas ativadas, esses fatores atuarão no sítio da lesão, estimulando a quimiotaxia, fibroplasia e angiogênese, melhorando assim a reparação tecidual. Embora esses componentes ricos em plaquetas sejam de fácil obtenção e de eficácia comprovada na medicina humana e odontologia, a utilização desses componentes na medicina veterinária é relativamente recente, necessitando ainda de estudos controlados. Neste artigo, os aspectos morfológicos das plaquetas, a ação dos fatores de crescimento e a utilização de componentes ricos em plaquetas na reparação tecidual de estruturas tendo-ligamentosas e osteo-articulares são revisados.Platelet-rich plasma and platelet concentrates are involved in growing factors. Both have great therapeutic potential. When the alpha-granules are released by the active platelet, they act in the lesion site stimulating the chemotaxis, the fiberplasia and the angiogenesis. They improve the regeneration of the tissue. Although these rich platelet components are easy to obtain and efficiently to prove by medicine and dentistry, their use on vet medicine is recent. So, much more control studies need to be done. This article reviews morphological aspects of platelets, action of growing factors and use of components rich in platelet in wound healing tendons, ligaments and osteo-articular structures.

Milhos com diferentes texturas de endosperma e adição de alfa-amilase na dieta de leitões The effect of corn kernel texture and alpha-amylase addition in performance and digestibility of diets for weaned pigs

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Vanessa Piovesan

2011-11-01

Full Text Available Dois experimentos foram realizados para avaliar a influência de milhos com diferentes texturas de endosperma e adição de alfa amilase na digestibilidade de dietas e no desempenho de leitões na fase de creche. No ensaio de digestibilidade, foram usados 12 leitões castrados (7,96±0,31kg, alojados individualmente em gaiolas de metabolismo, enquanto no desempenho utilizaram-se 75 leitões (7,87±0,67kg distribuídos em 15 gaiolas de creche. Nos dois experimentos, os tratamentos consistiram de três dietas formuladas para serem isonutritivas, mas com milhos de diferentes texturas e adição ou não de alfa amilase, conforme descrito a seguir: 1. grãos de milho semi-dentado; 2. grãos de milho duro e; 3. grãos de milho duro + alfa-amilase. Os resultados de metabolismo mostraram que os valores de energia digestível (ED e metabolizável (EM das dietas foram influenciados pelos tipos de milho, sendo o semi-dentado superior (P0,05 entre os tratamentos no consumo de ração, ganho de peso e/ou conversão alimentar. Dietas com milho duro ou semi-dentado e adição de alfa-amilase não alteram o desempenho dos leitões. Por outro lado, dietas contendo milho semi-dentado apresentam maior energia digestível que dietas com milho duro e a adição de alfa-amilase aumenta a energia de dietas com milho duro.Two experiments were carried out to evaluate the effects of diets with different corn grain textures and amylase supplementation on nutrients digestibility and nursery piglets' performance. Twelve castrated piglets (7.96±0.31kg were individually housed in metabolic crates and 75 piglets (7.87±0.67kg penned in 15 nursery pens. In both experiments the treatments consisted of three isonutritive diets: 1. semi dent corn grain; 2. flint corn grain, and 3. flint corn grain + amylase. There was effect of treatments (P0.05 the performance characteristics studied. In conclusion, diets with flint or semi dent corns and amylase supplementation did not improve

Alternative method for quantification of alfa-amylase activity Alternativa para quantificação de alfa-amilase

OpenAIRE

DF. Farias; AFU. Carvalho; CC. Oliveira; NM. Sousa; LCB. Rocha-Bezerrra; PMP. Ferreira; GPG. Lima; DC. Hissa

2010-01-01

A modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL-1. Clear radial diffusion zones were measured after 4 hours of incubation at 20 °C. A linear relationship between the logarithm of...

O papel do Fator de Necrose Tumoral Alfa (TNF-alfa no processo de erosão óssea presente no colesteatoma adquirido da orelha média The role of Tumor Necrosis Factor -Alpha (TNF- alpha in bone resorption present in middle ear cholesteatoma

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Rodrigo Faller Vitale

2007-02-01

Full Text Available O colesteatoma adquirido da orelha média causa erosão óssea, com altas taxas de morbidade e mortalidade. O TNF-alfa (TNF-alfa lambda uma das principais citocinas envolvidas neste processo. OBJETIVO: Avaliar o papel do TNF-alfa na reabsorsão óssea e a ação dele no colesteatoma. MATERIAL E MÉTODOS: Foi realizado um levantamento e uma revisão crítica da literatura. RESULTADOS: Todos os autores estudados concordam com a importância do TNF-alfa no processo de reabsorção óssea presente no colesteatoma e com o grau de destruição observado. Diferentes trabalhos demonstraram que o TNF-alfa é capaz de provocar erosão óssea, através de diferentes vias de ação. Ele pode estimular a diferenciação e a maturação dos osteoclastos ou, ainda, agir na matriz óssea expondo-a à ação dos osteoclastos. Existe a possibilidade de inibir a ação do TNF-alfa, diminuindo seus efeitos e prevenindo a perda óssea em doenças como a artrite reumatóide. Não existe, entretanto, trabalhos específicos em colesteatoma. Não existe consenso sobre a sua localização. Estas diferenças, provavelmente, ocorrem devido à distribuição dos receptores. CONCLUSÃO: O TNF-alfa, presente no colesteatoma promove a reabsorsão óssea, juntamente com outras citocinas (RANKL e IL-1, estando relacionado com a presença de complicações.Cholesteatoma may cause bone erosion, with high morbidity and mortality rates. Tumor Necrosis Factor -Alpha (TNF-a is one of the main cytokines involved in this process. Our goal was to evaluate the role of TNF-a in Bone Resorption and its effect on cholesteatoma. MATERIAL AND METHODS: analysis and critical literature review. RESULTS: Different studies have demonstrated that TNF-a is capable of causing bone erosion. It may stimulate the differentiation and maturation of osteoclasts or it may act on the bone matrix, exposing it to the action of the osteoclasts. It is possible to inhibit TNF-a, reducing its effects and prevent

Análise comparativa do controle interno no contas a receber e a pagar nas empresas Beta Ltda e Alfa Ltda = A comparative internal control analysis of receivable and payable accounts in Beta and Alfa Ltd Companies

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Cleyton de Oliveira Ritta

2010-07-01

Full Text Available A necessidade constante de um excelente desempenho empresarial leva à busca da qualidade e confiabilidade da informação para o auxílio na tomada de decisão. Neste contexto, a auditoria contábil tem um papel importante na análise das práticas administrativas adotadas pelas entidades. Desse modo, o objetivo deste artigo é comparar a estruturação dos controles internos no contas a receber e a pagar nas empresas Beta Ltda, a qual não contrata serviços de auditoria externa, e Alfa Ltda, que os contrata. Este estudo caracteriza-se como de caso comparativo de caráter descritivo por meio de questionário e entrevista. Os resultados apontam que a empresa Alfa Ltda tem melhores níveis de controle interno e, consequentemente, está menos exposta a erros e fraudes. Desse modo, entende-se que os seviços de auditoria atuam como um instrumento de auxílio para minimização dos riscos operacionais e servem de alerta aos gestores quanto à eficiência das transações. Contudo, percebe-se que a qualidade dos controles internos nas organizações depende diretamente da utilização de procedimentos de monitoramento.The constant need for excellent business performance triggers the quest for information quality and reliability to help in decision making. Considering this context, accounting auditing plays an important role in the analysis of administrative practices adopted by entities. Thus, the objective of this article is to compare the structure of the internal controls for receivable and payable accounts in Beta Ltd companies, which do not contract external auditing services, and Alfa Ltd, which does. This study is a descriptive comparative case study, carried out through questionnaire and interview. The results have showed that Alfa Ltd has better internal control levels and consequently is less exposed to errors and fraud. This way, auditing services are seen as auxiliary instruments to minimize operational risks and serve to alert

Strategicheskiy analiz predpriyatiya ZAO Alfa Motor Grupp

OpenAIRE

Antonova, Maria

2016-01-01

The subject of the bachelor work is the company ZAO Alfa Motor Group. The aim is to conduct strategic analysis of economic activity, evaluation of the effectiveness of current policies and develop recommendations and proposals to change the company's development strategy. ZAO Alfa Motor Group - a network of specialized service centers and car sales of spare parts, specialized in cars of Toyota and Lexus. Bachelor work has two main parts. In the first part of the work to uncover the meaning of...

Interferon alfa with or without ribavirin for chronic hepatitis C

DEFF Research Database (Denmark)

Kjaergard, L L; Krogsgaard, K; Gluud, C

2001-01-01

To assess the efficacy and safety of interferon alfa with or without ribavirin for treatment of chronic hepatitis C.......To assess the efficacy and safety of interferon alfa with or without ribavirin for treatment of chronic hepatitis C....

Adoption and de-adoption of drotrecogin alfa for severe sepsis in the United States.

Science.gov (United States)

Kahn, Jeremy M; Le, Tri Q

2016-04-01

Drotrecogin alfa was a landmark drug for treatment of severe sepsis, yet little is known about how it was adopted and de-adopted during its 10-year period of availability. We used hospitalization data on fee-for-service Medicare beneficiaries from 2002 to 2011 to characterize trends in the use of drotrecogin alfa in the United States. Drotrecogin alfa use peaked at 5.87 per 1000 severe sepsis hospitalizations in 2003 and then steadily declined to 0.94 administrations per 1000 severe sepsis hospitalizations in 2010. Large teaching hospitals were more likely to use drotrecogin alfa than small, nonteaching hospitals. The addition of "add-on payments" to hospitals for using drotrecogin alfa in 2002 was associated with significantly increased use (P publication of international sepsis guidelines with favorable drotrecogin alfa recommendations (in 2004 and 2008) nor the publication of a clinical trial focused on drotrecogin alfa (in 2005) were associated with consistent changes use (P > .05). Drotrecogin alfa use declined over time, with marked changes in use associated with drug-specific financial incentives but not the publication of clinical practice guidelines or clinical trials. Copyright © 2015 Elsevier Inc. All rights reserved.

Interferon alfa and ribavirin induced hair changes

International Nuclear Information System (INIS)

Amir, S.; Taj, A.; Muhamud, T.H.; Iqbal, Z.; Yaqub, F.

2007-01-01

Combination therapy of Interferon alfa and ribavirin in chronic hepatitis C has well documented cutaneous adverse effects. Most interesting of these has been reported on hair physiology. This study was conducted to determine the frequency and pattern of adverse effects involving hair in patients receiving combination of interferon alfa 2a and ribavirin for chronic hepatitis C. The study was conducted in Department of Dermatology, Division of Medicine Shaikh Zayed Hospital. Thirty Eight patients who completed treatment with interferon alfa (3 MIU subcutaneously thrice weekly) and 1200 mg ribavirin daily for 24 weeks were enrolled in this single-center study. The patient's response and examination finding particularly regarding involvement of hair was noted on a Proforma. Thirty Two out of thirty eight (84%) patients noted adverse effects involving hair. The most frequent was diffuse hair loss and occurred in 27 patients (71%). Hypertrichosis of eyelashes (trichomegaly) and eyebrows (synophyrs) was observed in 18 (47%) and 16 (42%) patients respectively. Graying of hair was noted in 4 patients (11%), while discoloration of moustache hair was seen in 2 patients (5%). Epilation at the site of subcutaneous injection was noted in 10 patients (26%). Alopecia areata was reported in 2 patients (5%). It is concluded that adverse effects involving hair are frequent and varied (hair loss to excess hair growth) during combination therapy with Interferon alfa-2a and Ribavirin for chronic hepatitis C. (author)

Rat hepatocyte invasion by Listeria monocytogenes and analysis of TNF-alpha role in apoptosis Invasão de hepatócitos de rato por Listeria monocytogenes e análise do papel do TNF-alfa na apoptose

Directory of Open Access Journals (Sweden)

Sânia Alves dos Santos

2005-04-01

do hospedeiro e manipulação do citoesqueleto, resultando em morte celular. As interações entre células do hospedeiro e bactérias podem resultar em produção de citocinas como o Fator de Necrose Tumoral alfa (TNF-alfa. Hepatócitos têm potencial de produzir citocinas pro-inflamatórias como TNF-alfa, quando invadidos por bactérias. No presente trabalho demonstramos o comportamento dos hepatócitos invadidos por L. monocytogenes pela análise microscópica, determinação da produção de TNF-alfa por bioensaio e análise da apoptose pela técnica TUNEL. A presença da bactéria, na razão que variou de 5 a 50.000 bactérias por célula, induziu ruptura das monocamadas celulares. Observamos presença de bactérias internalizadas na 1ª hora de incubação por microscopia eletrônica. Os níveis de TNF-alfa aumentaram da 1ª hora de incubação até a 6ª hora, variando de 0 a 3749 pg/mL. Nas 7ª e 8ª horas de incubação, ocorreram quedas não significativas dos níveis de TNF-alfa, indicando possível saturação dos receptores celulares. A quantidade de TNF-alfa produzido por hepatócitos foi dependente do tempo de incubação, assim como da proporção entre bactérias e células. A taxa de apoptose aumentou diretamente com o tempo de incubação (1 h a 8 + 24 h, variando de 0 a 43%, assim como com a razão bactérias : células. Estes resultados mostram a habilidade de L. monocytogenes não-hemolítica em invadir os hepatócitos, e as principais conseqüências deste fenômeno são: liberação de TNF-alfa e indução de apoptose. Assim, podemos especular que hepatócitos usam apoptose induzida por TNF-alfa para liberar bactérias de seu interior, facilitando a destruição destas pelo sistema imune.

Managing anemia with epoetin alfa in patients with rectal cancer

International Nuclear Information System (INIS)

Velenik, V.; Oblak, I.; Kodre, V.

2005-01-01

Background. Anemia is one of the most challenging problems in clinical oncology due to its high prevalence among the patients with malignant diseases. The purposes of our study were: (1) to assess the potential of epoetin alfa therapy to prevent the decline in Hb concentrations that typically accompanies chemotherapy/ radiotherapy (ChT/RT) of the patients with rectal cancer; (2) to test the hypothesis that the use of epoetin alfa significantly reduces the transfusion requirements in the patients with rectal cancer treated with ChT/RT after surgery, and (3) to evaluate the safety profile of the administration of epoetin alfa in the clinical setting. Methods. Sixty patients who underwent surgery for rectal cancer were prospectively enrolled. Group A consisted of 39 patients with Hb concentrations ≤13 g/dl at the start of ChT/RT following surgery, and group B of 17 patients with Hb concentrations ≥13 g/dl at the start of ChT/RT following surgery, but whose Hb concentrations fell below 13 g/dl during the ChT/RT protocol. The starting dose of epoetin alfa in both groups was 10,000 IU subcutaneously (sc) three times a week (tiw). The following major parameters were evaluated: (1) change in Hb concentrations relative to the baseline as measured at 4-week intervals, (2) allogenic blood transfusion requirements in relation to Hb concentrations, and (3) incidence and severity of adverse events and their potential relationship to epoetin alfa administration. Results. The study protocol was completed in 56/60 patients. In group A, a statistically significant increase in Hb concentration (p<0.001) was observed after the first 4 weeks of epoetin alfa treatment compared to the baseline values, with the mean increase of Hb concentration of 1.97 g/dl ± 0.91 g/dl and Hb concentrations remained significantly increased through the whole study (p=0.0017). In group B, a continuous decrease in Hb concentrations was observed during the first weeks of therapy, reaching the level of

Darbepoetin alfa once every 2 weeks for treatment of anemia in dialysis patients

DEFF Research Database (Denmark)

Mann, J; Kessler, M; Villa, Giulio Palludan

2007-01-01

AIM: Darbepoetin alfa has a longer half-life than epoetin-(EPO) alfa or beta, allowing administration at less frequent intervals for the treatment of renal anemia. The aim of the present analysis was to evaluate the efficacy and tolerability of an every-2-week (Q2W) schedule of darbepoetin alfa...

Efeitos da suplementação de probióticos na prevenção da obesidade e suas complicações em camundongos Swiss

OpenAIRE

Renata Alvares Bagarolli Zambon

2013-01-01

Resumo: A obesidade é caracterizada por processo inflamatório crônico e resistência à insulina (RI), os quais são responsáveis por grande parte de suas doenças associadas. Sabe-se que diversas moléculas do sistema imune inato estão associadas à RI e obesidade, destacando-se o receptor toll-like-receptor 4 (TLR4). Sua via de sinalização está ativada na obesidade, devido à presença aumentada na circulação de seu principal ligante, lipopolissacarídeo (LPS). Acredita-se que esta endotoxemia metab...

Upgrade of ATLAS-ALFA for Run2

CERN Document Server

Jakobsen, Sune; The ATLAS collaboration

2016-01-01

Early experience with the operation of the ALFA detectors in the LHC environment has shown significant beam-induced heating. Comprehensive studies on the effects of heating with a spare detector module have furthermore revealed that heating effects could be disastrous in the case of the larger beam intensities foreseen for higher luminosity. A temporary solution was implemented and kept the detectors below the critical temperature in 2012. In LS1 all ALFA stations have been removed from LHC and are undergoing an upgrade of the geometry of Roman Pots to minimize the impedance losses. When the stations were re-installed ~4 m distance was added between them to gain level arm in the angular measurement between the stations. The updated geometry of the Roman Pot, together with a system to improve the internal heat transfer and an air cooling system, is expected to keep the temperatures of ALFA detectors below critical limits throughout the LHC Run2 and first results from the performance in 2016 will be presented. ...

Avaliação do desempenho dos reagentes do tempo de tromboplastina parcial ativada utilizados para detectar o anticoagulante lúpico Assessment of the performance of reagents of activated partial thromboplastin time used to detect the lupus anticoagulant

Directory of Open Access Journals (Sweden)

Fernanda Chiuso

2005-06-01

Full Text Available INTRODUÇÃO: O anticoagulante lúpico é uma imunoglobulina pertencente à família dos anticorpos antifosfolípides. A sua ação in vitro é interferir nos testes de coagulação dependentes de fosfolípides. O tempo de tromboplastina parcial ativada (TTPA é um teste utilizado como screening na pesquisa do anticoagulante lúpico. Os reagentes utilizados neste teste apresentam grandes variações quanto à sensibilidade. OBJETIVO: Avaliar o desempenho dos reagentes do TTPA e detectar a presença do anticoagulante lúpico através de diferentes testes da coagulação. MATERIAL E MÉTODO: A pesquisa do anticoagulante lúpico foi realizada em 50 amostras plasmáticas de pacientes do sexo feminino através dos testes do TTPA, do tempo de coagulação do caulim (TCC, do tempo de tromboplastina parcial ativada diluída (TTPAd e do tempo do veneno da víbora de Russel diluído (TVVRd. Três cefalinas comerciais foram avaliadas pelos testes do TTPA e do TTPAd. Na comparação entre os reagentes estudados foi aplicado o cálculo do intervalo de confiança (95%. RESULTADOS: Os três reagentes avaliados apresentaram boa concordância e os métodos utilizados responderam bem à pesquisa do anticoagulante lúpico. DISCUSSÃO E CONCLUSÃO: As três cefalinas comerciais avaliadas podem ser utilizadas na rotina laboratorial para a pesquisa do anticoagulante lúpico.INTRODUCTION: The lupus anticoagulant is an immunoglobin which belongs to the antiphospholid antibodies family. Its in vitro function is to interfere with coagulation tests that are dependent on phospholipids. The activated partial thromboplastin time (APTT is a test used as screening on lupus anticoagulant research. Reagents used in this test demonstrate wide sensitivity ranges. OBJECTIVE: To assess the performance of APTT reagents and detect the presence of lupus anticoagulant through various coagulation tests. MATERIAL AND METHOD: The lupus anticoagulant research was performed in plasma from 50

A trial of darbepoetin alfa in type 2 diabetes and chronic kidney disease

DEFF Research Database (Denmark)

Pfeffer, Marc A; Burdmann, Emmanuel A; Chen, Chao-Yin

2009-01-01

BACKGROUND: Anemia is associated with an increased risk of cardiovascular and renal events among patients with type 2 diabetes and chronic kidney disease. Although darbepoetin alfa can effectively increase hemoglobin levels, its effect on clinical outcomes in these patients has not been adequately...... tested. METHODS: In this study involving 4038 patients with diabetes, chronic kidney disease, and anemia, we randomly assigned 2012 patients to darbepoetin alfa to achieve a hemoglobin level of approximately 13 g per deciliter and 2026 patients to placebo, with rescue darbepoetin alfa when the hemoglobin...... assigned to darbepoetin alfa and 496 patients assigned to placebo (Pchronic kidney disease...

Rendimento e processo germinativo do grão na espiga de genótipos de trigo

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Felicio João Carlos

2002-01-01

Full Text Available O objetivo deste trabalho foi estudar o processo germinativo dos grãos na espiga, o rendimento de grãos, a adaptabilidade e a estabilidade em genótipos de trigo (Triticum aestivum L. sensíveis às variações ambientais. Os experimentos foram instalados no Núcleo Experimental do Instituto Agronômico, em Campinas, SP, no período de 1996 a 1998. A germinação na espiga foi avaliada pelo método do "Falling Number", que consiste em determinar o nível de atividade da alfa-amilase nos grãos. A segunda e a terceira colheitas foram realizadas ao sete e quatorze dias após a primeira colheita. O atraso da colheita determinou redução no rendimento, notadamente na terceira colheita; os teores de alfa-amilase foram maiores também na terceira colheita, em virtude da degradação dos grãos expostos ao tempo. O genótipo IAC 289 apresentou adaptabilidade a ambientes favoráveis, mas apresentou suscetibilidade à ocorrência de germinação dos grãos na espiga. Os genótipos IAC 24, Mochis, IAC 370 e IAC 351 foram os mais resistentes à germinação dos grãos na espiga, apresentando baixa atividade de alfa-amilase.

Immunogenicity of glycans on biotherapeutic drugs produced in plant expression systems—The taliglucerase alfa story

Science.gov (United States)

Rup, Bonita; Alon, Sari; Amit-Cohen, Bat-Chen; Brill Almon, Einat; Chertkoff, Raul; Rudd, Pauline M.

2017-01-01

Plants are a promising alternative for the production of biotherapeutics. Manufacturing in-planta adds plant specific glycans. To understand immunogenic potential of these glycans, we developed a validated method to detect plant specific glycan antibodies in human serum. Using this assay, low prevalence of pre-existing anti-plant glycan antibodies was found in healthy humans (13.5%) and in glucocerebrosidase-deficient Gaucher disease (GD) patients (5%). A low incidence (9% in naïve patient and none in treatment experienced patients) of induced anti-plant glycan antibodies was observed in GD patients after up to 30 months replacement therapy treatment with taliglucerase alfa, a version of human glucocerebrosidase produced in plant cells. Detailed evaluation of clinical safety and efficacy endpoints indicated that anti-plant glycan antibodies did not affect the safety or efficacy of taliglucerase alfa in patients. This study shows the benefit of using large scale human trials to evaluate the immunogenicity risk of plant derived glycans, and indicates no apparent risk related to anti-plant glycan antibodies. PMID:29088235

Alpha absolute power: motor learning of practical pistol shooting Potência absoluta de alfa: aprendizagem motora do tiro prático de pistola

Directory of Open Access Journals (Sweden)

Clayton Amaral Domingues

2008-06-01

Full Text Available The present study aimed at investigating changes in behavior (shooting precision and electrophysiological variables (absolute alpha power during the motor learning of practical pistol shooting. The sample was composed of 23 healthy subjects, right-handed, male, between 18 and 20 years of age. The task consisted of four learning blocks. A One-way ANOVA with repeated measures and a post hoc analysis were employed to observe modifications on behavioral and electrophysiological measures (pO presente estudo teve por objetivo investigar alterações nas variáveis comportamentais (precisão do tiro e eletrofisiológicas (potência absoluta de alfa durante o aprendizado motor do tiro prático de pistola. A amostra constituiu-se de 20 sujeitos saudáveis, destros, sexo masculino, faixa etária entre 18 e 20 anos. A tarefa consistiu de quatro blocos de aprendizagem. A análise estatística das variáveis comportamentais e eletrofisiológicas foram realizadas por meio de uma ANOVA one-way e uma análise post hoc (p<0,05. Os resultados demonstraram diferenças significativas entre os blocos em função do aprendizado motor, bem como uma sensível melhora na precisão do tiro de ambos os blocos. Foi observada uma diminuição na potência de alfa em todos os eletrodos analisados durante a execução da tarefa, quando comparados aos blocos de linha de base e controle da aprendizagem. Os achados sugerem que a potência em alfa diminui devido o aprendizado motor quando sujeitos estão engajados na execução de uma tarefa motora complexa.

Análise da utilização na prática dos termos atribuídos aos fenômenos de enfermagem da CIPE-Versão Alfa

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Maria Miriam Lima da Nóbrega

2001-09-01

Full Text Available Este estudo teve como propósito a análise da utilização dos termos atribuídos aos fenômenos de enfermagem na prática das enfermeiras paraibanas. Os resultados evidenciam que todos os termos são utilizados pelas enfermeiras, com alguma freqüência. No que diz respeito à utilização dos termos, de acordo com o estrato amostral, os resultados evidenciam que as enfermeiras dos demais Municípios Paraibanos utilizam um maior número de termos, estatisticamente significativo, quando comparado com os dois outros estratos - João Pessoa e Campina Grande. A partir dos resultados do estudo, pode-se concluir, que os termos atribuídos aos fenômenos constantes na CIPE - Versão Alfa representam problemas ou situações da prática de enfermagem das enfermeiras paraibanas, corroborando a afirmação do Conselho Internacional de Enfermeiras de que estes termos são comuns às enfermeiras de todo o mundo.

Analgesia regional periférica com lidocaína em paciente queimado: relato de caso

Directory of Open Access Journals (Sweden)

Geier Karl Otto

2004-01-01

Full Text Available JUSTIFICATIVA E OBJETIVOS: A realização de anestesia regional em pacientes queimados é dificultosa pela localização aleatória das lesões térmicas. Elevadas taxas de alfa1 glicoproteína ácida com grande afinidade para drogas alcalinas, especialmente a lidocaína, têm sido observadas nesses pacientes. Este caso relata como o uso intermitente de anestesia e analgesia regional periférica com altas doses de lidocaína podem ser úteis em fornecer efetiva analgesia num paciente com queimaduras de segundo grau nos quatro membros, abrangendo, aproximadamente, 20% de área superficial queimada. RELATO DO CASO: Paciente do sexo masculino, 23 anos, 86 kg, estado físico ASA II, com queimadura superficial da face, segundo grau nos quatro membros e elevada taxa sérica de alfa1 glicoproteína ácida (260 mg.dl-1, teve sua dor controlada com 11,6 mg.kg-1 de lidocaína com adrenalina 1:400.000 administrada por cateteres introduzidos e tunelizados para diversos procedimentos - irrigações e troca de curativos, desbridamentos, fisioterapia, enxertos cutâneos e analgesia diária durante 28 dias. CONCLUSÕES: Em pacientes queimados com injúrias térmicas localizadas nas extremidades, a analgesia regional periférica pode ser útil. As elevadas taxas séricas de alfa1 glicoproteína ácida e o local da injeção podem permitir o emprego de altas doses de lidocaína.

"Alfa" i "Võmpel" bez maski / Andrei Sharov

Index Scriptorium Estoniae

Sharov, Andrei

2004-01-01

Venemaa FSB keskuse, mille koosseisu kuuluvad ka eriüksused Alfa ja Võmpel, töötajad osalevad terroristide neutraliseerimise ja pantvangide vabastamise erioperatsioonides. Intervjuu keskuse staabi uue juhiga

Treatment of anemia with darbepoetin alfa in systolic heart failure

DEFF Research Database (Denmark)

Swedberg, Karl; Young, James B; Anand, Inder S

2013-01-01

Patients with systolic heart failure and anemia have worse symptoms, functional capacity, and outcomes than those without anemia. We evaluated the effects of darbepoetin alfa on clinical outcomes in patients with systolic heart failure and anemia.......Patients with systolic heart failure and anemia have worse symptoms, functional capacity, and outcomes than those without anemia. We evaluated the effects of darbepoetin alfa on clinical outcomes in patients with systolic heart failure and anemia....

Resolution and t-spectrum for the ALFA detector

CERN Document Server

Guergouri, Hichem

2016-01-01

My report as Summer Student is in two parts. In the first part I report about my attendance to the run of the ALFA detector to test different collimation schemas and find a new way to get rid of the background. The second part is about the measurement of the resolution of the ALFA detector and comparing the real data with the Monte-Carlo (MC) simulations. First of all I tried to be more familiar with ROOT, worked on understanding the code used to access to the data in the trees inside the Ntuples, plotting histograms,… This was done using old Ntuples which contain MC data.

Influência da vitamina D associada ao interferon alfa-peguilado e ribavirina na resposta virológica sustentada na hepatite C crônica

OpenAIRE

Luiza Vitelo ANDRIGHETTO; Jacqueline Shaurich dos SANTOS; Graziella NICOLETTI; Patricia Martins BOCK

2016-01-01

O objetivo deste trabalho foi avaliar a influência da vitamina D no tratamento de hepatite C com interferon-alfa peguilado e ribavirina. Foram utilizados artigos científicos relevantes publicados entre os anos de 2000 e 2015. Em diversos estudos foi observado que níveis mais elevados de vitamina D estavam associados à rápida resposta ao tratamento e à maior taxa de RVS, sugerindo que a deficiência de vitamina D pode estar ligada a uma falta de resposta ao tratamento antiviral. A i...

Dynamic behavior of reactive aluminum nanoparticle-fluorinated acrylic (AlFA) polymer composites

Science.gov (United States)

Crouse, Christopher A.; White, Brad; Spowart, Jonathan E.

2011-06-01

The dynamic behavior of aluminum nanoparticle-fluorinated acrylic (AlFA) composite materials has been explored under high strain rates. Cylindrical pellets of the AlFA composite materials were mounted onto copper sabots and impacted against a rigid anvil at velocities between 100 and 400 m/s utilizing a Taylor gas gun apparatus to achieve strain rates on the order of 104 /s. A framing camera was used to record the compaction and reaction events that occurred upon contact of the pellet with the anvil. Under both open air and vacuum environments the AlFA composites demonstrated high reactivity suggesting that the particles are primarily reacting with the fluorinated matrix. We hypothesize, based upon the compaction history of these materials, that reaction is initiated when the oxide shells on the aluminum nanoparticles are broken due an interparticle contact deformation process. We have investigated this hypothesis through altering the particle loading in the AlFA composites as well as impact velocities. This data and the corresponding trends will be presented in detail.

Frutificação e crescimento de frutos em abóbora híbrida 'Tetsukabuto' tratada com alfa-naftalenoacetato de sódio Fruit set and fruit growth of 'Tetsukabuto' squash treated with the sodium salt of alfa-naphthaleneacetic acid

Directory of Open Access Journals (Sweden)

Cassandro V.T. do Amarante

2000-11-01

Full Text Available A abertura de flores femininas na abóbora híbrida 'Tetsukabuto' concentra-se num período de três a quatro semanas. Este curto período de florescimento indica que uma polinização deficiente pode ocasionar grande comprometimento da produção de frutos. Este trabalho foi conduzido com o objetivo de avaliar o efeito de concentrações do fitorregulador alfa-naftalenoacetato de sódio (ANA-Na, pulverizado em flores abertas femininas, na frutificação e crescimento de frutos da abóbora 'Tetsukabuto'. O experimento foi conduzido em Lages, SC, na safra 1992/1993, seguindo o delineamento em blocos casualizados, com três repetições. A parcela foi constituída de cinco covas de 'Tetsukabuto' e uma da polinizadora Cucurbita maxima, em espaçamento de 3 x 1 m, com duas plantas/cova. Os tratamentos utilizados foram testemunha (polinização natural e 150, 300, 450, 600 e 750 mg·L-1 de ANA-Na. Nos tratamentos com ANA-Na, evitou-se a polinização natural isolando-se as flores com sacos de papel. Avaliou-se percentagem de frutificação, peso, comprimento e diâmetro dos frutos e peso, espessura, rendimento e sólidos totais da polpa. Apenas a dose de 750 mg·L-1 do hormônio reduziu a percentagem de frutificação. Os frutos obtidos por tratamento com ANA-Na apresentaram crescimento similar ao dos frutos obtidos por polinização.Anthesis of female flowers of 'Tetsukabuto' squash occurs within three to four weeks. A deficient pollination during this short flowering period may reduce the yield. The sodium salt of alfa-naphthaleneacetic acid (Na-NAA can be used to improve fruit set when pollination is deficient. This work was conducted to investigate the effects of different concentrations of Na-NAA, sprayed in open female flowers on fruit set and fruit growth of 'Tetsukabuto' squash. The experiment was carried out in 1992-93, in South Brazil, in a randomized block design with three replicates. The plots had a proportion of five plants of

Um imunoensaio rápido para a detecção de alfa-trembolona em urina bovina

Directory of Open Access Journals (Sweden)

Keila Maria Roncato Duarte

2006-12-01

Full Text Available O Brasil está entre os países que mantêm o uso de anabolizantes proibido, o que gera dificuldades muito grandes no monitoramento destas substâncias, uma vez que possui o maior rebanho bovino. Contudo, diversas substâncias são largamente utilizadas, entre as quais o acetato de trembolona. Com o consentimento do Ministério da Agricultura, Pecuária e Abastecimento, foram produzidos anticorpos policlonais para um teste baseado em ELISA ("enzyme linked immunosorbent assay" capazes de detectar acetato de trembolona na urina de bovinos tratados com trembolona. O teste apresentou baixo custo e de fácil execução para a detecção de alfa-trembolone, o principal metabólito na absorção do acetato de trembolona, liberado na urina dos animais. Os resultados foram similares aos obtidos com o "kit" comercial usado pelo Ministério da Agricultura, Pecuária e Abastecimento do Brasil.

Collaborative study for the validation of an improved HPLC assay for recombinant IFN-alfa-2.

Science.gov (United States)

Jönsson, K H; Daas, A; Buchheit, K H; Terao, E

2016-01-01

The current European Pharmacopoeia (Ph. Eur.) texts for Interferon (IFN)-alfa-2 include a nonspecific photometric protein assay using albumin as calibrator and a highly variable cell-based assay for the potency determination of the protective effects. A request was expressed by the Official Medicines Control Laboratories (OMCLs) for improved methods for the batch control of recombinant interferon alfa-2 bulk and market surveillance testing of finished products, including those formulated with Human Serum Albumin (HSA). A HPLC method was developed at the Medical Products Agency (MPA, Sweden) for the testing of IFN-alfa-2 products. An initial collaborative study run under the Biological Standardisation Programme (BSP; study code BSP039) revealed the need for minor changes to improve linearity of the calibration curves, assay reproducibility and robustness. The goal of the collaborative study, coded BSP071, was to transfer and further validate this improved HPLC method. Ten laboratories participated in the study. Four marketed IFN-alfa-2 preparations (one containing HSA) together with the Ph. Eur. Chemical Reference Substance (CRS) for IFN-alfa-2a and IFN-alfa-2b, and in-house reference standards from two manufacturers were used for the quantitative assay. The modified method was successfully transferred to all laboratories despite local variation in equipment. The resolution between the main and the oxidised forms of IFN-alfa-2 was improved compared to the results from the BSP039 study. The improved method even allowed partial resolution of an extra peak after the principal peak. Symmetry of the main IFN peak was acceptable for all samples in all laboratories. Calibration curves established with the Ph. Eur. IFN-alfa-2a and IFN-alfa-2b CRSs showed excellent linearity with intercepts close to the origin and coefficients of determination greater than 0.9995. Assay repeatability, intermediate precision and reproducibility varied with the tested sample within acceptable

A redesigned follitropin alfa pen injector for infertility: results of a market research study

Directory of Open Access Journals (Sweden)

Abbotts C

2011-06-01

Full Text Available Carole Abbotts1, Cristiana Salgado-Braga2, Céline Audibert-Gros31Pharmaceutical Marketing Research Consultancy, London, UK; 2Fertility and Endocrinology Global Business Unit, 3Business Intelligence, Merck Serono SA, Geneva, SwitzerlandBackground: The purpose of this study was to evaluate patient-learning and nurse-teaching experiences when using a redesigned prefilled, ready-to-use follitropin alfa pen injector.Methods: Seventy-three UK women of reproductive age either administering daily treatment with self-injectable gonadotropins or about to start gonadotropin treatment for infertility (aged 24–47 years; 53 self-injection-experienced and 20 self-injection-naïve and 28 nurses from UK infertility clinics were recruited for the study. Following instruction, patients and nurses used the redesigned follitropin alfa pen to inject water into an orange and completed questionnaires to evaluate their experiences with the pen immediately after the simulated injections.Results: Most (88%, n = 64 patients found it easy to learn how to use the pen. Among injection-experienced patients, 66% (n = 35 agreed that the redesigned pen was easier to learn to use compared with their current method and 70% (n = 37 also said they would prefer its use over current devices for all injectable fertility medications. All nurses considered the redesigned pen easy to learn and believed it would be easy to teach patients how to use. Eighty-six percent (n = 24 of the nurses thought it was easy to teach patients to determine the remaining dose to be dialed and injected in a second pen if the initial dose was incomplete. Compared with other injection devices, 96% (n = 27 thought it was "much easier" to "as easy" to teach patients to use the redesigned pen. Based on ease of teaching, 68% (n = 19 of nurses would choose to teach the pen in preference to any other injection method. Almost all (93%, n = 26 nurses considered that having the same pen format for a range of

A large, multicentre, observational, post-marketing surveillance study of the 2:1 formulation of follitropin alfa and lutropin alfa in routine clinical practice for assisted reproductive technology.

Science.gov (United States)

Bühler, Klaus; Naether, Olaf G J; Bilger, Wilma

2014-01-14

Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) both have a role to play in follicular development during the natural menstrual cycle. LH supplementation during controlled ovarian stimulation (COS) for assisted reproductive technology (ART) is used for patients with hypogonadotropic hypogonadism. However, the use of exogenous LH in COS in normogonadotropic women undergoing ART is the subject of debate. The aim of this study was to investigate characteristics of infertile women who received the 2:1 formulation of follitropin alfa and lutropin alfa (indicated for stimulation of follicular development in women with severe LH and FSH deficiency) in German clinical practice. A 3-year, multicentre, open-label, observational/non-interventional, post-marketing surveillance study of women (21-45 years) undergoing ART. Primary endpoint: reason for prescribing the 2:1 formulation of follitropin alfa and lutropin alfa. Secondary variables included: COS duration/dose; oocytes retrieved; fertilization; clinical pregnancy; ovarian hyperstimulation syndrome (OHSS). In total, 2220 cycles were assessed; at least one reason for prescribing the 2:1 formulation was given in 1834/2220 (82.6%) cycles. Most common reasons were: poor ovarian response (POR) (39.4%), low baseline LH (17.8%), and age (13.8%). COS: mean dose of the 2:1 formulation on first day, 183.1/91.5 IU; mean duration, 10.8 days. In 2173/2220 (97.9%) cycles, human chorionic gonadotrophin was administered. Oocyte pick-up (OPU) was attempted in 2108/2220 (95.0%) cycles; mean (standard deviation) 8.0 (5.4) oocytes retrieved/OPU cycle. Fertilization (≥1 oocyte fertilized) rates: in vitro fertilization (IVF), 391/439 (89.1%) cycles; intracytoplasmic sperm injection (ICSI)/IVF + ICSI, 1524/1613 (94.5%) cycles. Clinical pregnancy rate: all cycles, 25.9%; embryo transfer cycles, 31.3%. OHSS: hospitalization for OHSS, 8 (0.36%) cycles, Grade 2, 60 (2.7%), and Grade 3, 1 (0.05%). In German routine clinical

Calibration, testing, commissioning and first data of ALFA at LHC

CERN Document Server

Jakobsen, S; The ATLAS collaboration

2011-01-01

The ALFA detector system (Absolute Luminosity For ATLAS) aims for measuring the absolute LHC luminosity with high precision by using pp-scattering under small angles. Scintillating fibers tracking detectors are positioned 240 m from LHC interaction point 1 inside Roman Pots at millimeter distance from the LHC beam axis. The detectors consist of layers of 64 scintillating square fibers of 500 µm size in a U-V configuration. The layers are staggered 10 times to improve the spatial resolution to about 30 micrometers. A total of 11680 scintillating fibers are read out on the 8 ALFA detectors through 184 Multi Anode Photomultiplier Tubes, MAPMTs, of type Hamamatsu R7600-00-M64, 64 channels. Each detector has dedicated scintillating trigger tiles read out by 8 mm Hamamatsu Photomultiplier Tubes (R7400P and R9880U-110) via clear fibers. The gain of the MAPMTs differs up to a factor 3 inside a MAPMT and an additional factor 2 between different MAPMTs. For compensation, ALFA has developed a front-end electronic based...

Evaluating the transport layer of the ALFA framework for the Intel® Xeon Phi™ Coprocessor

Science.gov (United States)

Santogidis, Aram; Hirstius, Andreas; Lalis, Spyros

2015-12-01

The ALFA framework supports the software development of major High Energy Physics experiments. As part of our research effort to optimize the transport layer of ALFA, we focus on profiling its data transfer performance for inter-node communication on the Intel Xeon Phi Coprocessor. In this article we present the collected performance measurements with the related analysis of the results. The optimization opportunities that are discovered, help us to formulate the future plans of enabling high performance data transfer for ALFA on the Intel Xeon Phi architecture.

Arroz integral, seleno-levedura e acetato de alfa-tocoferol na dieta de frangos de corte: efeitos sobre a qualidade da carne

Directory of Open Access Journals (Sweden)

Aline Arassiana Piccini Roll

2017-05-01

Full Text Available Foram estudados os efeitos da suplementação com acetato de alfa-tocoferol (VE e seleno-levedura (SeL Sel-Plex, Alltech® Inc, sobre o pH, a capacidade de retenção de água (CRA, perdas por cocção (PC, perdas por gotejamento (PG, cor do músculo e a concentração de selênio no peito de frangos alimentados com dietas a base de milho ou arroz. A partir de 21 dias de idade 200 frangos de corte Cobb foram alojados em 38 boxes (unidade experimental num delineamento casualizado num arranjo fatorial 2x2x2 em que foram fixados os níveis de suplementação “on top” de VE (0 e 200 mg/kg, SeL (0 e 0,3 ppm e dois ingredientes da dieta (100% milho e 100% arroz integral totalizando oito tratamentos: T1 milho + 0SeL + 0VE (controle; T2 milho + 200mg/kg VE + 0SeL; T3 milho + 0VE + 0,3ppm SeL; T4 milho + 200mg/kg VE + 0,3ppm SeL; T5 arroz + 0VE +0SeL; T6 arroz + 200mg/kg VE + 0SeL; T7 arroz + 0VE + 0,3ppm SeL; T8 arroz + 200mg/kg VE + 0,3ppm SeL. A quantidade de selênio no peito foi maior (P < 0,0001 com a inclusão de 200mg/kg de VE, em comparação com os demais tratamentos. Entretanto, observou-se uma interação positiva entre VE e SeL na dieta sobre a quantidade de selênio recuperada na carne (P = 0,06. Foi encontrada melhor CRA com a inclusão de SeL e VE em dietas a base de arroz. A substituição do milho por arroz nas dietas reduziu a cor amarela na carne (P < 0,05. PC e PG não foram significativamente afetadas pelos tratamentos. O pH da carne do peito foi significativamente mais elevado nas aves recebendo dietas suplementadas de SeL. Em conclusão, a interação entre VE e SeL aumenta o selênio na carne, porém, melhorando a CRA somente em dietas a base de arroz. A substituição de milho por arroz integral reduz a intensidade da coloração amarela da carne do peito de frangos.

Treatment-naïve Gaucher disease patients achieve therapeutic goals and normalization with velaglucerase alfa by 4years in phase 3 trials.

Science.gov (United States)

Zimran, Ari; Elstein, Deborah; Gonzalez, Derlis E; Lukina, Elena A; Qin, Yulin; Dinh, Quinn; Turkia, Hadhami Ben

2018-02-01

Gaucher disease is an inherited metabolic disease characterized by β-glucocerebrosidase deficiency and commonly treated with enzyme replacement therapy (ERT). The efficacy of ERT with velaglucerase alfa was assessed based on the achievement of published therapeutic goals and the normalization of disease parameters in 39 treatment-naïve patients with type 1 Gaucher disease, 6 to 62years of age, enrolled in phase 3 clinical trials. After 4years of ERT, therapeutic goals for thrombocytopenia and splenomegaly had been achieved in 100% of patients; goals for anemia and hepatomegaly had been achieved in 95% and 94% of patients, respectively. Consistent with the goal for bone mineral density, lumbar spine bone density improved in 87% of patients ≥18years of age. At year 4, compared with clinical ranges for healthy individuals, 86% of patients with a low baseline hemoglobin concentration had normalized, 60% with a low baseline platelet count had normalized, 67% with baseline splenomegaly had normalized, 58% with hepatomegaly had normalized, and lumbar spine bone density had normalized in 53% of adults. The decade-old therapeutic goals do not reflect the potential for normalization of clinical parameters in ERT-treated patients. Goals consistent with normalization or near-normalization should be considered. ClinicalTrials.gov identifiers: NCT00430625, NCT00553631, NCT00635427. Copyright © 2016 Shire Human Genetic Therapies, Inc. Published by Elsevier Inc. All rights reserved.

Pilot study of dornase alfa (Pulmozyme) therapy for acquired ventilator-associated infection in preterm infants.

Science.gov (United States)

Scala, Melissa; Hoy, Deborah; Bautista, Maria; Palafoutas, Judith Jones; Abubakar, Kabir

2017-06-01

Evaluate the feasibility, safety, and efficacy of adjunctive treatment with dornase alfa in preterm patients with ventilator-associated pulmonary infection (VAPI) compared to standard care. We hypothesize that therapy with dornase alfa will be safe and well tolerated in the preterm population with no worsening of symptoms, oxygen requirement, or need for respiratory support. Prospective, randomized, blinded, pilot study comparing adjunctive treatment with dornase alfa to sham therapy. In addition to standard care, infants were randomized to receive dornase alfa 2.5 mg nebulized via endotracheal tube (ETT) every 12 hr for 7 days or sham therapy. ETT secretion gram stain and culture and chest X-ray (CXR) findings were evaluated. Respiratory support data were downloaded from the ventilator. Fourteen infants developed VAPI between 2012 and 2014; 11 enrolled in the study. Six received dornase alfa and five received sham therapy. Average gestational age at birth was 25 weeks and age at study entry was 31 days. There were no differences in demographics, ETT white blood cell count (WBC), CXR, or mean airway pressure (MAP) between the two groups. There was a trend towards decreased oxygen requirement (FiO2) in the treatment group that did not reach statistical significance. No side effects were observed in the treatment group. Treatment with dornase alfa is safe and treated infants had some improvement in FiO 2 requirement but no improvement in MAP. A larger randomized trial is needed to evaluate the efficacy of this therapy. Pediatr Pulmonol. 2017; 52:787-791. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Assessment of Relative Bioavailability of Moroctocog Alfa and Moroctocog Alfa (AF-CC) in Subjects With Severe Hemophilia A.

Science.gov (United States)

Korth-Bradley, Joan; Rupon, Jeremy; Plotka, Anna; Charnigo, Robert; Rendo, Pablo

2018-05-01

An open-label, single-dose, randomized, two-period, crossover study comparing the pharmacokinetics of factor VIII activity in plasma (FVIII:C) after administration of an albumin-free presentation of moroctocog alfa (test) and moroctocog alfa manufactured using the previous technique (reference) was conducted in 30 (25 evaluable) male subjects who had severe hemophilia A (FVIII:C < 1 IU/dL). Blood samples were collected for 48 h after administration of each dose. C was assayed using a chromogenic substrate assay. The FVIII:C pharmacokinetic parameters were calculated using noncompartmental analysis. The presentations would be bioequivalent if the 90% confidence limits of the ratio of the geometric mean values of AUC inf and recovery fell within the interval of 80-125%. The bioequivalence criteria were met. A total of 10 treatment-related adverse events were observed in a total of nine subjects. All were mild and none was determined to be related to administration of study medication. © 2018 The Authors. Clinical and Translational Science published by Wiley Periodicals, Inc. on behalf of American Society for Clinical Pharmacology and Therapeutics.

Unidades fototérmicas e temperatura-base inferior de frutos de Mangueira Alfa, na Baixada Cuiabana Photothermal units and lower base temperature for alfa mango fruit on the Baixada Cuiabana

Directory of Open Access Journals (Sweden)

Marcelo Paes de Barros

2010-06-01

Full Text Available O objetivo deste trabalho foi determinar a temperatura-base inferior (Tb a partir da unidade fototérmica (UF para o cultivo experimental de manga Alfa sob condições de cerrado. Foram utilizados dados diários da temperatura do ar disponibilizados pela Estação Agrometeorológica Padre Ricardo Remetter, localizada em Santo Antônio do Leverger-MT (15,8° S e 56,1° W, 140 m, e avaliação sensorial do estádio de maturação dos frutos de manga. A Tb foi determinada pelo método da menor variabilidade das unidades fototérmicas (UF acumuladas do período da floração à colheita dos frutos, variabilidade avaliada pelo coeficiente de variação (cv simulado para diferentes valores da Tb. De julho a novembro de 2007, em três plantas de um pomar demonstrativo irrigado, foram identificados 82 frutos para o acompanhamento do crescimento e maturação. Entre esses frutos, em meados de dezembro de 2007, foi possível identificar 13 frutos que atingiram a maturidade fisiológica, após um período médio de observação de 112 dias. Para exigência fototérmica de 1.878.166,1 UF, encontrou-se Tb de 10 °C, valor consistente com os apresentados na literatura para a cultivar de manga, o que comprova a eficiência do método que combina a ação da temperatura e do fotoperíodo sobre a maturação dos frutos e que confere um caráter mais racional que o método tradicional da soma térmica.In this study was to determine the lower base temperature (Tb for Alfa mango fruit, using the photothermal units (PTU method in cerrado conditions. The meteorological data for the study were acquired from "Padre Ricardo Remetter" meteorological station located at Santo Antonio do Leverger, MT, Brazil (15,8° S and 56,1° W, 140 m, and the fruit data was acquired by sensorial evaluation of mango fruit maturation state. The Tb was determined by less variability of the coefficient of variation (cv method applied to the PTU sum, from flowering to harvest, simulated for

Methods in ALFA Alignment

CERN Document Server

Melendez, Jordan

2014-01-01

This note presents two model-independent methods for use in the alignment of the ALFA forward detectors. Using a Monte Carlo simulated LHC run at \\beta = 90m and \\sqrt{s} = 7 TeV, the Kinematic Peak alignment method is utilized to reconstruct the Mandelstam momentum transfer variable t for single-diractive protons. The Hot Spot method uses fluctuations in the hitmap density to pinpoint particular regions in the detector that could signal a misalignment. Another method uses an error function fit to find the detector edge. With this information, the vertical alignment can be determined.

Hyporesponsiveness to Darbepoetin Alfa in Patients With Heart Failure and Anemia in the RED-HF Study (Reduction of Events by Darbepoetin Alfa in Heart Failure): Clinical and Prognostic Associations.

Science.gov (United States)

van der Meer, Peter; Grote Beverborg, Niels; Pfeffer, Marc A; Olson, Kurt; Anand, Inder S; Westenbrink, B Daan; McMurray, John J V; Swedberg, Karl; Young, James B; Solomon, Scott D; van Veldhuisen, Dirk J

2018-02-01

A poor response to erythropoiesis-stimulating agents such as darbepoetin alfa has been associated with adverse outcomes in patients with diabetes mellitus, chronic kidney disease, and anemia; whether this is also true in heart failure is unclear. We performed a post hoc analysis of the RED-HF trial (Reduction of Events by Darbepoetin Alfa in Heart Failure), in which 1008 patients with systolic heart failure and anemia (hemoglobin level, 9.0-12.0 g/dL) were randomized to darbepoetin alfa. We examined the relationship between the hematopoietic response to darbepoetin alfa and the incidence of all-cause death or first heart failure hospitalization during a follow-up of 28 months. For the purposes of the present study, patients in the lowest quartile of hemoglobin change after 4 weeks were considered nonresponders. The median initial hemoglobin change in nonresponders (n=252) was -0.25 g/dL and +1.00 g/dL in the remainder of patients (n=756). Worse renal function, lower sodium levels, and less use of angiotensin-converting enzyme inhibitors or angiotensin receptor blockers were independently associated with nonresponse. Although a low endogenous erythropoietin level helped to differentiate responders from nonresponders, its predictive value in a multivariable model was poor (C statistic=0.69). Nonresponders had a higher rate of all-cause death or first heart failure hospitalization (hazard ratio, 1.25; 95% confidence interval, 1.02-1.54) and a higher risk of all-cause mortality (hazard ratio, 1.30; 95% confidence interval, 1.04-1.63) than responders. A poor response to darbepoetin alfa was associated with worse outcomes in heart failure patients with anemia. Patients with a poor response were difficult to identify using clinical and biochemical biomarkers. URL: https://www.clinicaltrials.gov. Unique identifier: NCT00358215. © 2018 American Heart Association, Inc.

JSC “ALFA-BANK” marketing policy. problems and perspectives

OpenAIRE

Kirillov, A.; Kuznetcova, E.; Martirosian, M.

2013-01-01

The article is devoted to the results of JSC “Alfa-Bank” consumers’ segmentation and the following complex marketing research. The article suggests the ways of the bank’s marketing policy improvement.

Análise de crédito por meio de modelos de previsão de insolvência: um estudo de caso na Empresa Cerâmica Alfa S.A. = Credit analysis through models for the forcasting of insolvency of the company Cerâmica Alfa S.A.

Directory of Open Access Journals (Sweden)

Paula Bez Birolo

2011-07-01

Full Text Available O objetivo deste artigo é avaliar os benefícios da utilização de modelos de previsão de insolvência para a análise de crédito em uma empresa do setor cerâmico. É descritivo, de caráter quantitativo, realizado por meio de um estudo de caso na empresa Cerâmica ALFA S.A. Para execução da pesquisa selecionou-se intencionalmente seis clientes, de acordo com os seguintes dados: a informações creditícias disponíveis no sistema gerencial de crédito/cobrança e b demonstrações contábeis divulgadas nos sites dos respectivos clientes. Os resultados apontam que os modelos de Elizabetsky e Kanitz demonstram fragilidades em identificar a situação financeira dos clientes. O modelo de previsão de insolvência desenvolvido por Matias apresenta melhor desempenho, em relação aos anteriores, para a análise de crédito. Conclui-se que é vantajoso utilizar o modelo de Matias para a análise de crédito, pois ele é adequado às necessidades da empresa e serve como um instrumento para auxílio na concessão de crédito e tomada de decisão. The objective of this article is to assess the benefits of using insolvency forecasting models for the credit analysis of a company in the ceramic sector. This study is descriptive of a quantitative nature through a case study in Cerâmica ALFA S.A. Six clients with the following data were intentionally selected for the execution of the research: a credit information available in the credit/collection management system and b account statements divulged in the sites of the respective clients. The results show that the Elizabetsky and Kanitz models demonstrated fragility in identifying the financial situation of the clients. The insolvency forecasting model developed by Matias showed better performance in relation to the previous ones for credit analysis. It was concludrd that it is advantageous to use the Matias model for credit analysis, because it is suitable for the company’s requirements and serves

Drotrecogin alfa (activated) in adults with septic shock.

NARCIS (Netherlands)

Ranieri, V.M.; Thompson, B.T.; Barie, P.S.; Dhainaut, J.F.; Douglas, I.S.; Finfer, S.; Gardlund, B.; Marshall, J.C.; Rhodes, A.; Artigas, A.; Payen, D.; Tenhunen, J.; Al-Khalidi, H.R.; Thompson, V.; Janes, J.; Macias, W.L.; Vangerow, B.; Williams, M.D.; Pickkers, P.; Raemaekers, J.M.; et al.,

2012-01-01

BACKGROUND: There have been conflicting reports on the efficacy of recombinant human activated protein C, or drotrecogin alfa (activated) (DrotAA), for the treatment of patients with septic shock. METHODS: In this randomized, double-blind, placebo-controlled, multicenter trial, we assigned 1697

ATLAS-ALFA measurements on the total cross section and diffraction

CERN Document Server

Mortensen, Simon Stark; The ATLAS collaboration

2015-01-01

The measurement of the total pp cross section at the LHC at $\\sqrt{s}=7$ TeV with the ALFA subdetector of ATLAS is presented in this talk. In a special run with $\\beta^*=90$ m beam optics corresponding to an integrated luminosity of 80 $\\text{mb}^{-1}$ the differential elastic cross section is measured in the range from $-t=0.0025\\text{ GeV}^2$ to $-t=0.38\\text{ GeV}^2$. The total cross section $\\sigma(pp\\rightarrow X)$ is extracted using the Optical Theorem by extrapolation of the differential elastic cross section to $t=0\\text{ GeV}^2$. Prospects for diffractive measurements using ALFA to detect the intact proton(s) is also discussed.

The in vitro mucolytic effect of xylitol and dornase alfa on chronic rhinosinusitis mucus.

Science.gov (United States)

Hardcastle, Tim; Jain, Ravi; Radcliff, Fiona; Waldvogel-Thurlow, Sharon; Zoing, Melissa; Biswas, Kristi; Douglas, Richard

2017-09-01

The overproduction and stagnation of purulent mucus impair mucociliary clearance and exacerbate the symptoms of chronic rhinosinusitis (CRS). There is a clinical need for effective topical mucolytic agents to facilitate removal of mucus and improve postoperative outcomes. The effects of xylitol (5%) and dornase alfa (1 mg/mL) on mucus and mucus crusts were investigated. Viscoelasticity and viscosity of wet mucus derived from 30 CRS patients was measured with a plate rheometer. Postoperative dried mucus crust dissolution was measured by examining peripheral transparency, central transparency, and border definition of treated crust samples from 17 CRS patients. Xylitol and dornase alfa reduced wet mucus viscoelasticity at a frequency of 0.1 Hz significantly more than the saline control. Treatments also produced significantly lower viscosities than saline at a shear rate of 10 and 100 seconds -1 . Xylitol and dornase alfa significantly decreased mucus crust border definition relative to saline. Xylitol and dornase alfa may be efficacious mucolytics, encouraging the breakdown of postoperative mucus crusts and the reduction of viscoelasticity and viscosity of wet mucus. In vivo study is required to evaluate the potential of these agents in treating recalcitrant CRS. © 2017 ARS-AAOA, LLC.

Alternative method for quantification of alfa-amylase activity.

Science.gov (United States)

Farias, D F; Carvalho, A F U; Oliveira, C C; Sousa, N M; Rocha-Bezerrra, L C B; Ferreira, P M P; Lima, G P G; Hissa, D C

2010-05-01

A modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL-1. Clear radial diffusion zones were measured after 4 hours of incubation at 20 A degrees C. A linear relationship between the logarithm of enzyme activities and the area of clear zones was obtained. The method was validated by testing alpha-amylase from barley at the concentrations of 2.4; 60; 300 and 1,500 U.mL-1. The proposed method turned out to be simpler, faster, less expensive and able to determine on a macro-scale alpha-amylase over a wide range (2.4 to 7,500 U.mL-1) in scientific investigation as well as in teaching laboratory activities.

Alternative method for quantification of alfa-amylase activity

Directory of Open Access Journals (Sweden)

DF. Farias

Full Text Available A modification of the sensitive agar diffusion method was developed for macro-scale determination of alfa-amylase. The proposed modifications lower costs with the utilisation of starch as substrate and agar as supporting medium. Thus, a standard curve was built using alfa-amylase solution from Aspergillus oryzae, with concentrations ranging from 2.4 to 7,500 U.mL-1. Clear radial diffusion zones were measured after 4 hours of incubation at 20 °C. A linear relationship between the logarithm of enzyme activities and the area of clear zones was obtained. The method was validated by testing α-amylase from barley at the concentrations of 2.4; 60; 300 and 1,500 U.mL-1. The proposed method turned out to be simpler, faster, less expensive and able to determine on a macro-scale α-amylase over a wide range (2.4 to 7,500 U.mL-1 in scientific investigation as well as in teaching laboratory activities.

Drotrecogin alfa (activated in severe sepsis: a systematic review and new cost-effectiveness analysis

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Brophy James M

2007-06-01

Full Text Available Abstract Background Activated drotrecogin alfa (human activated protein C, rhAPC, is produced by recombinant DNA technology, and purports to improve clinical outcomes by counteracting the inflammatory and thrombotic consequences of severe sepsis. Controversy exists around the clinical benefits of this drug and an updated economic study that considers this variability is needed. Methods A systematic literature review was performed using Medline, Embase and the International Network of Agencies for Health Technology Assessment (INAHTA databases to determine efficacy, safety and previous economic studies. Our economic model was populated with systematic estimates of these parameters and with population life tables for longer term survival information. Monte Carlo simulations were used to estimate the incremental cost-effectiveness ratios (ICERs and variance for the decision analytic models. Results Two randomized clinical trials (RCTS of drotrecogin alfa in adults with severe sepsis and 8 previous economic studies were identified. Although associated with statistical heterogeneity, a pooled analysis of the RCTs did not show a statistically significant 28-day mortality benefit for drotrecogin alfa compared to placebo either for all patients (RR: 0.93, 95% CI: 0.69, 1.26 or those at highest risk as measured by APACHE II ≥ 25 (RR: 0.90, 95% CI: 0.54, 1.49. Our economic analysis based on the totality of the available clinical evidence suggests that the cost-effectiveness of drotrecogin alfa is uncertain ( Conclusion The evidence supporting the clinical and economic attractiveness of drotrecogin alfa is not conclusive and further research appears to be indicated.

Avaliação da orientação motivacional de metas na modalidade do futebol

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Vinicius Barroso Hirota

2013-07-01

O presente trabalho objetivou avaliar e comparar a orientação motivacional de meta para tarefa e ego de alunos pertencentes a dois ambientes esportivos distintos de futebol, uma Escola de Esporte Privada e uma Organização Não Governamental (ONG, ambos localizados na Cidade de Osasco - SP. Através de uma pesquisa descritiva aplicamos a escala TEOSQ, a uma amostra de 196 sujeitos de ambos os sexos de idade entre 7 e 17 anos (média 11,16+2,09 e os dados foram tratados com o calculo do Coeficiente Alfa, média, desvio padrão, mediana e comparadas as médias com o teste de Mann Whitney. Os resultados mostraram estabilidade no instrumento com Alfa de 0,78 para tarefa e 0,68 de ego na escola de esporte com média de 4,28 de tarefa e 2,40 de ego, já a ONG o Alfa de tarefa foi 0,60 e 0,74 de ego com média de tarefa de 4,40 e ego 2,75. Concluímos a tendência de orientação para tarefa com alunos predispostos a se superarem constantemente, e a fidedignidade do instrumento

New Synthesis of .alfa.,.omega.-Diiodoalkynes and Capped Iodobutadiynes

Czech Academy of Sciences Publication Activity Database

Hlavatý, Jaromír; Kavan, Ladislav; Štícha, M.

- (2002), s. 705-706 ISSN 1472-7781 R&D Projects: GA ČR GA203/00/0634 Institutional research plan: CEZ:AV0Z4040901 Keywords : .alfa.,. omega .-diiodoalkynes * capped iodobutadiynes Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 2.181, year: 2002

Pathophysiology of hypophosphatasia and the potential role of asfotase alfa

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Orimo H

2016-05-01

Full Text Available Hideo Orimo Division of Metabolism and Nutrition, Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan Abstract: Hypophosphatasia (HPP is an inherited systemic bone disease that is characterized by bone hypomineralization. HPP is classified into six forms according to the age of onset and severity as perinatal (lethal, perinatal benign, infantile, childhood, adult, and odontohypophosphatasia. The causative gene of the disease is the ALPL gene that encodes tissue-nonspecific alkaline phosphatase (TNAP. TNAP is expressed ubiquitously, and its physiological role is apparent in bone mineralization. A defect in bone mineralization can manifest in several ways, including rickets or osteomalacia in HPP patients. Patients with severe forms suffer from respiratory failure because of hypoplastic chest, which is the main cause of death. They sometimes present with seizures due to a defect in vitamin B6 metabolism resulting from the lack of alkaline phosphatase activity in neuronal cells, which is also lethal. Patients with a mild form of the disease exhibit rickets or osteomalacia and a functional defect of exercise. Odontohypophosphatasia shows only dental manifestations. To date, 302 mutations in the ALPL gene have been reported, mainly single-nucleotide substitutions, and the relationships between phenotype and genotype have been partially elucidated. An established treatment for HPP was not available until the recent development of enzyme replacement therapy. The first successful enzyme replacement therapy in model mice using a modified human TNAP protein (asfotase alfa was reported in 2008, and subsequently success in patients with severe form of the disease was reported in 2012. In 2015, asfotase alfa was approved in Japan in July, followed by in the EU and Canada in August, and then by the US Food and Drug Administration in the USA in October. It is expected that therapy with asfotase alfa will drastically change

Analgesia regional periférica com lidocaína em paciente queimado: relato de caso

OpenAIRE

Geier, Karl Otto

2004-01-01

JUSTIFICATIVA E OBJETIVOS: A realização de anestesia regional em pacientes queimados é dificultosa pela localização aleatória das lesões térmicas. Elevadas taxas de alfa1 glicoproteína ácida com grande afinidade para drogas alcalinas, especialmente a lidocaína, têm sido observadas nesses pacientes. Este caso relata como o uso intermitente de anestesia e analgesia regional periférica com altas doses de lidocaína podem ser úteis em fornecer efetiva analgesia num paciente com queimaduras de segu...

Possibilities of uranium recovering as by product of copper lixiviation solution in Salobo 3-Alfa-Carajas

International Nuclear Information System (INIS)

Hohn, H.; Ferraioli, R.N.M.

1983-05-01

Geochemical and geophysics regional surveys performed by Nuclebras in 'Carajas, Para' State, show several favourable areas for uranium prospecting, including the 'Salobo 3-Alfa' copper mine. Some studies for increasing the Knowledge of uranium quantity and proportion and for accompanying the pilot plant installation, aiming the uranium extraction during the cycel of ore treatment in 'Salobo 3-Alfa' mine are described [pt

A New Approach to the ALFA Trigger Simulator

CERN Document Server

Dziedzic, Bartosz

2016-01-01

The idea and principle of operation of a device which is used to test the trigger system of the ALFA detectors of the ATLAS experiment at the LHC are discussed. A new approach to the application control is presented. The application runs under control of the ArchLinuxARM operating system. Also, the drawbacks of the new solution are discussed.

Efficacy of Alfa EEG wave biofeedback in the management of anxiety

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Pookala Bhat

2010-01-01

Full Text Available Background: Biofeedback is a technique in which people are trained to improve their health by learning to control certain internal bodily processes that normally occur involuntarily. Various studies in the past have shown usefulness of Alfa electroencephalographic (EEG biofeedback in the alleviation of anxiety symptoms. Though most of the psychiatric centers in the armed forces have this facility, not much work has been done in our setup to assess its efficacy in the management of anxiety. Hence this study was undertaken. Materials and Methods: This study was carried out in a multispecialty Command Hospital by enrolling 100 patients with psychiatric diagnosis from both inpatient and outpatient services. The anxiety level was assessed clinically and by using Hamilton Anxiety Scale and Taylor′s Manifest Anxiety Scale. One group of 50 patients was treated with Alfa EEG biofeedback sessions only, 5 times in a week for 8 weeks, along with specific pharmacotherapy. The other group was treated with appropriate dose of anxiolytics. The anxiety level was reassessed after 4 weeks and 8 weeks. Results: The response was better for mixed anxiety and depressive disorder with pharmacotherapy than with the biofeedback, but female patients showed better response with EEG biofeedback. Conclusion: In the short term, Alfa EEG biofeedback therapy is almost as efficacious as pharmacological intervention in the management of anxiety symptoms, and relatively more useful in females.

Alfa tahab TeliaSonerat Türgis üle pakkuda / Tõnis Arnover

Index Scriptorium Estoniae

Arnover, Tõnis, 1952-

2005-01-01

TeliaSoneraga, kellele kuulub 37 protsenti Türgi operaatorfirma Turkcell aktsiatest, konkureerib täiendava 27 protsendise aktsiapaki ostmises Venemaa operaatorfirmaga Alfa Telecom. Lisa: Kaasosanike huvid põrkuvad. Diagramm: Konkurendid Vene MegaFonis

Carbonaceous Polymers and Nanotubes from .alfa., .omega.-Dialkali Hexatriynides

Czech Academy of Sciences Publication Activity Database

Hlavatý, Jaromír; Kavan, Ladislav; Okabe, K.; Oya, A.

2002-01-01

Roč. 40, č. 7 (2002), s. 1147-1150 ISSN 0008-6223 R&D Projects: GA ČR GA203/00/0634 Institutional research plan: CEZ:AV0Z4040901 Keywords : carbon nanotubes * chemically modified carbon * .alfa.,. omega .-dialkali hexatriynides Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 3.048, year: 2002

Tratamento de hemangioma gigante com interferon alfa: relato de dois casos Treatment of giant hemangioma with interferon-alpha: report of two cases

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Ana Julia Balau

2007-12-01

Full Text Available O objetivo do trabalho é descrever o uso de interferon alfa no tratamento de pacientes com hemangioma gigante. Os autores relatam e analisam dois casos de hemangioma gigante em tratamento com interferon alfa. IBS, 3 anos, em acompanhamento no Ambulatório de Hematologia desde um ano de idade com quadro de lesão angiomatosa em praticamente toda hemiface direita, acompanhada de sangramentos gengivais importantes. Após a realização de exames complementares (Angiorressonância magnética e feito o diagnóstico de hemangioma gigante em face, foi iniciado tratamento com prednisona e, posteriormente, associação com interferon alfa e observada importante melhora do quadro, resultando na diminuição dos episódios de sangramento e no tamanho do tumor. C.N.P., 12 anos, apresentando nódulo em região lateral de joelho esquerdo há 2 anos, com aumento progressivo do tamanho e dor local. Fez uso de prednisona e, sem melhora do quadro, introduzido interferon alfa com regressão importante do tamanho do tumor. O tratamento com interferon alfa deve ser considerado no tratamento de hemangiomas, pois apresenta bons resultados em relação à diminuição do tamanho do tumor e, conseqüentemente, reduz as intercorrências clínicas associadas à sua presença, principalmente os sangramentos.The aim of this study is to describe the treatment using interferon-alpha of giant hemangiomas in children. The authors report two cases of children presenting with giant hemangiomas treated using interferon-alpha and analyze the results. IBS, 3 years-old, has been followed up in Famema Hemathology Service since she was 1 year-old with a tumor on the face and persistent bleeding. After clinical and radiologic evaluations and suggested the diagnosis of giant hemangioma, she started treatment with interferon-alpha. A great clinical improvement was observed a reducing of the number of episodes of bleedings and a decrease in of the tumor size. CNP, 12 years-old, came to

Espectroscopia Mössbauer e refinamento estrutural no sistema cerâmico isomórfico (alfa-Fe2O3-(alfa-Al2O 3 Mössbauer spectroscopy and structural refinement in the isomorphic (alpha-Fe2O3-( alpha-Al2O3 ceramic system

Directory of Open Access Journals (Sweden)

J. A. Moreto

2007-06-01

Full Text Available Os multiferróicos são materiais em que duas ou três propriedades tais como ferroeletricidade, ferromagnetismo e ferroelasticidade são observadas em uma mesma fase. Em particular, os multiferróicos magnetoelétricos representam os materiais que são simultaneamente (antiferromagnéticos e ferroelétricos, com ou sem ferroelasticidade. Especificamente, o sistema hematita (alfa-Fe2O3-alumina (alfa-Al2O3 tem sido estudado, principalmente devido às suas potencialidades para aplicações em metalurgia e como catalisador na síntese de amônia. Contudo, compostos deste sistema também podem apresentar efeitos magnetoelétricos. Neste trabalho um estudo estrutural do composto (alfa-Fe2O30,25(alfa-Al2O 30,75 submetido à moagem em altas energias e tratamento térmico pós-moagem é apresentado. As amostras foram caracterizadas por difração de raios X, refinamento estrutural Rietveld e espectroscopia Mössbauer. A determinação e identificação das fases cristalográficas, parâmetros de rede e volume de celas unitárias nas amostras moída e tratada termicamente permitiu verificar as mudanças dos parâmetros, a formação de espinélios e possíveis deformações relativas advindas do processo de moagem em altas energias.Multiferroics are materials in which two or all three of the properties, ferroelectricity, ferromagnetism, and ferroelasticity occur in the same phase. In particular, multiferroic magnetoelectrics represent the materials that are simultaneously ferromagnetic and ferroelectric, with or without ferroelasticity. Specifically, the hematite (alpha-Fe2O3-alumina (alpha-Al2O3 system has been studied, mainly due to its potential applications in metallurgy and as catalyst of ammonia synthesis. However, compounds of this system also may present magnetoelectric effects. In this work, a structural study of the high-energy ball milled and annealed (alpha-Fe2O3 0.25(alpha-Al2O30.75 compound was carefully conducted. The samples were

The pharmacokinetics of a B-domain truncated recombinant factor VIII, turoctocog alfa (NovoEight®), in patients with hemophilia A.

Science.gov (United States)

Jiménez-Yuste, V; Lejniece, S; Klamroth, R; Suzuki, T; Santagostino, E; Karim, F A; Saugstrup, T; Møss, J

2015-03-01

Turoctocog alfa (NovoEight(®)) is a human recombinant coagulation factor VIII (rFVIII) for the treatment of patients with hemophilia A. To evaluate the pharmacokinetics of turoctocog alfa in all age groups across clinical trials. Data from previously treated patients with severe hemophilia A (FVIII activity level of ≤ 1%) with no history of FVIII inhibitors, in a non-bleeding state, were included. The pharmacokinetics were assessed following a wash-out period and a subsequent single intravenous 50 IU kg(-1) dose of turoctocog alfa. Blood was sampled during a 48-h period postdose. Standard pharmacokinetic (PK) parameters were estimated on the basis of plasma FVIII activity vs. time (PK profiles) with non-compartmental methods. Furthermore, a population PK analysis was conducted. Data from 76 patients (aged 1-60 years) enrolled globally across six clinical trials were included, totaling 105 turoctocog alfa PK profiles. Single-dose PK results 3-6 months after the first dose of turoctocog alfa were comparable with the results obtained after the first dose. Similar PK characteristics were shown for different lots and strengths of the drug product. Overall, area under the plasma concentration (activity) curve from administration to infinity (AUC) and t1(/2) tended to increase with increasing age, with lower AUC and shorter t(1/2) being seen in children than in adolescents and adults. The PK profiles of turoctocog alfa and other commercially available plasma-derived FVIII and rFVIII products were similar in all age groups. The PK characteristics of turoctocog alfa have been thoroughly studied, and shown to be consistent over time, reproducible between different lots and strengths of drug product, and similar to those observed for other FVIII products. © 2014 International Society on Thrombosis and Haemostasis.

Autoantibodies to alfa-fodrin in patients with Hashimoto thyroiditis and Sjögren's syndrome: possible markers for a common secretory disorder.

Science.gov (United States)

Szanto, Antonia; Csipo, Istvan; Horvath, Ildiko; Biro, Edit; Szodoray, Peter; Zeher, Margit

2008-09-01

Presence of autoantibodies to alfa-fodrin was investigated in patients with Sjögren's syndrome (n = 61), Hashimoto thyroiditis (n = 27), Sjögren's syndrome associated with Hashimoto thyroiditis (n = 31) and in healthy persons (n = 77). In each group, level of alfa-fodrin antibodies was higher than in the controls. There was no significant difference in their presence either between patients with Hashimoto thyroiditis with or without Sjögren's syndrome, or-in IgA isotype-between Sjögren's and Hashimoto thyroiditis patients. Correlation was found between the level of IgG alfa-fodrin and anti-thyroglobulin antibodies. Based on these findings, fodrin can be associated with both endocrine and exocrine glandular secretion. Antibodies to alfa-fodrin might have a role in the pathogenesis of Hashimoto thyroiditis concerning the "final common effectory pathway", secretion. Alfa-fodrin antibodies can be good markers of secretory disorders. Assessment of these autoantibodies might help the diagnosis and follow-up of patients with impaired secretory capability of not only autoimmune origin.

Efeito da suplementação com vitamina E sobre a concentração de alfa-tocoferol no colostro humano Effect of vitamin E supplementation on alpha-tocopherol levels in human colostrum

Directory of Open Access Journals (Sweden)

Roberto Dimenstein

2011-06-01

Full Text Available OBJETIVO: Avaliar a concentração de alfa-tocoferol no colostro humano em condições de suplementação com cápsulas de vitamina A acrescidas de vitamina E sintética. MÉTODOS: Foram recrutadas para o estudo 30 parturientes saudáveis atendidas em uma maternidade pública. Após jejum noturno, foram coletadas amostras de sangue e de colostro (2 mL das parturientes. Após a primeira coleta, as mesmas receberam suplemento na forma de uma cápsula de palmitato de retinila (200000 UI ou 60 mg acrescido de vitamina E sintética (49,4 mg de dl-alfa-tocoferol. Após 24 horas da suplementação, foi realizada nova coleta de 2 mL de colostro, também em jejum. RESULTADOS: A concentração sérica de alfa-tocoferol foi de 1 042,9 ± 319,0 μg/dL. Os teores de alfa-tocoferol no colostro antes da suplementação foram de 1155,4 ± 811,0 μg/dL, vs. 1396,3 ± 862,2 μg/dL 24 horas depois da suplementação (P > 0,05. Foi encontrada correlação entre o alfa-tocoferol do colostro na condição de jejum antes da suplementação e 24 horas após a suplementação (P = 0,001; r = 0,58, mas não entre soro e o colostro em nenhuma das condições acima citadas. CONCLUSÕES: Não houve aumento na concentração de alfa-tocoferol do colostro 24 horas após a suplementação. Isso indica que não é vantajoso suplementar a mãe com vitamina E sintética. Entretanto, os resultados também sugerem que, se o estado nutricional prévio à suplementação estiver adequado, mais tocoferol será encontrado no colostro após a suplementação. Novos estudos devem ser realizados para investigar o efeito da suplementação com a forma natural do alfa-tocoferol.OBJECTIVE: To assess the levels of alpha-tocopherol in human colostrum following supplementation with capsules containing vitamin A plus synthetic vitamin E. METHODS: Thirty healthy women about to give birth were recruited from a public maternity hospital. After overnight fasting, blood samples as well as colostrum

De-squeeze the beams: the TOTEM and ATLAS/ALFA experiments

CERN Multimedia

Stefania Pandolfi

2016-01-01

A special week-long proton–proton run with larger beam sizes at the interaction point is intended to probe the p-p elastic scattering regime at small angles.   Nicola Turini, deputy spokesperson for TOTEM, in front of one of the experiment’s ‘Roman Pot’ detectors in the LHC tunnel. (Photo: Maximilien Brice/CERN) Usually, the motto of the LHC is “maximum luminosity”. But for a few days per year, the LHC ignores its motto to run at very low luminosity for the forward experiments. This week, the LHC will provide the TOTEM and ATLAS/ALFA experiments with data for a broad physics programme. The TOTEM experiment at Point 5 and the ATLAS/ALFA experiment at Point 1 study the elastic scattering of protons, which are not observable in normal operation runs. In the elastic scattering process, the two protons survive their encounter intact and only change directions by exchanging momentum. To allow this special run, the operators play with the so-c...

Measurement of the total cross section with ALFA Detector at ATLAS

CERN Document Server

Trzebinski, M; The ATLAS collaboration

2017-01-01

The main goals of the Absolute Luminosity For ATLAS (ALFA) detector is to provide an absolute luminosity and total cross section measurement. The measurement method used, the detector alignment and the quality of the collected data are discussed.

Efeitos do levosimendan sobre TNF-alfa, PNB e MMP-1 em pacientes com insuficiência cardíaca com anemia

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Mutlu Büyüklü

2012-07-01

Full Text Available FUNDAMENTO: O levosimendan é conhecido pelo seu efeito bilateral de fortalecimento contração das miofibrilas sem aumentar a demanda de oxigênio no miocárdio. A anemia é uma deterioração que causa aumento da dosagem de fármacos em pacientes com insuficiência cardíaca. OBJETIVO: No presente estudo comparamos a eficácia do tratamento com levosimendan em pacientes com insuficiência cardíaca descompensada com ou sem anemia. MÉTODOS: Foram incluídos no estudo 23 pacientes anêmicos com insuficiência cardíaca classe 3 ou 4, segundo a New York Heart Association (NYHA e fração de ejeção abaixo de 35%. Outros 23 pacientes com o mesmo diagnóstico cardíaco, mas sem anemia, serviu como grupo controle. Ao tratamento da insuficiência cardíaca tradicional desses pacientes foi acrescido um tratamento de 24 horas de levosimendan. Amostras foram tomadas para dosar os níveis séricos do fator de necrose tumoral alfa sérico (TNF-alfa, peptídeo natriurético cerebral aminoterminal (NT-proPNB e metaloproteinase da matriz 1 (MMP-1, antes e após a administração. RESULTADOS: Não houve diferença significativa entre os níveis séricos de TNF-alfa e MMP-1, antes e depois do tratamento (p > 0,05. Embora o nível de NT-proBNP tenha diminuído em ambos os grupos após o tratamento, não foi estatisticamente significativo (p = 0,531 e p = 0,913 para os grupos de anemia e de controle, respectivamente. Uma restauração significativa da capacidade funcional foi observada em ambos os grupos avaliados, de acordo com a NYHA (p < 0,001 e p = 0,001 para os grupos de anemia e controle, respectivamente. CONCLUSÃO: O tratamento com levosimendan apresenta efeitos semelhantes em pacientes com insuficiência cardíaca, com anemia e sem anemia. No entanto, o efeito precoce desse tratamento sobre os níveis de TNF-alfa, NT-proPNB e MMP-1 não é evidente. Ele oferece uma melhora significativa na capacidade funcional, sem a influência da anemia.

The role of corifollitropin alfa in controlled ovarian stimulation for IVF in combination with GnRH antagonist

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Seyhan A

2011-08-01

Full Text Available Ayse Seyhan, Baris AtaDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, McGill University, Montreal, QC, CanadaAbstract: Corifollitropin alfa is a synthetic recombinant follicle-stimulating hormone (rFSH molecule containing a hybrid beta subunit, which provides a plasma half-life of ∼65 hours while maintaining its pharmocodynamic activity. A single injection of corifollitropin alfa can replace daily FSH injections for the first week of ovarian stimulation for in vitro fertilization. Stimulation can be continued with daily FSH injections if the need arises. To date, more than 2500 anticipated normoresponder women have participated in clinical trials with corifollitropin alfa. It is noteworthy that one-third of women did not require additional gonadotropin injections and reached human chorionic gonadotropin criterion on day 8. The optimal corifollitropin dose has been calculated to be 100 µg for women with a body weight ≤ 60 kg and 150 µg for women with a body weight >60 kg, respectively. Combination of corifollitropin with daily gonadotropin-releasing hormone antagonist injections starting on stimulation day 5 seems to yield similar or significantly higher numbers of oocytes and good quality embryos, as well as similar ongoing pregnancy rates compared with women stimulated with daily rFSH injections. Stimulation characteristics, embryology, and clinical outcomes seem consistent with repeated corifollitropin-stimulated assisted reproductive technologies cycles. Multiple pregnancy or ovarian hyperstimulation syndrome rates with corifollitropin were not increased over daily FSH regimen. The corifollitropin alfa molecule does not seem to be immunogenic and does not induce neutralizing antibody formation. Drug hypersensitivity and injection-site reactions are not increased. Incidence and nature of adverse events and serious adverse events are similar to daily FSH injections. Current trials do not

Evaluating the transport layer of the ALFA framework for the Intel(®) Xeon Phi(™) Coprocessor

OpenAIRE

Santogidis, Aram; Hirstius, Andreas; Lalis, Spyros

2015-01-01

The ALFA framework supports the software development of major High Energy Physics experiments. As part of our research effort to optimize the transport layer of ALFA, we focus on profiling its data transfer performance for inter-node communication on the Intel Xeon Phi Coprocessor. In this article we present the collected performance measurements with the related analysis of the results. The optimization opportunities that are discovered, help us to formulate the future plans of enabling high...

Valorization of Tunisian alfa fibres and sumac tannins for the elaboration of biodegradable insulating panels

Science.gov (United States)

Saad, Houda; Charrier, Bertrand; Ayed, Naceur; Charrier-El-Bouhtoury, Fatima

2017-10-01

Alfa leaves are important renewable raw materials in Tunisia where they are used basically in handcrafts and paper industry. Sumac is also an abundant species in Tunisia known for its high tannin content and is basically used in traditional medicine. To valorize these natural resources, we studied, for the first time, the possibility of making insulating panels based on alfa fibres and sumac tannins based adhesive. Firstly, alfa leaves were treated with an alkali solution as it is one of the standard procedures commonly used in the paper industry to extract cellulosic fibres. Mercerization effects were studied by characterizing fibres thermal properties and fibres surface morphology. Secondly, the sumac tannin based resin was formulated and characterized. Finally, the insulating panel was elaborated and characterized by determining its thermal conductivity. The thermal gravimetric analysis results show improvement in the thermal stability of fibres after alkali treatment. Environmental Scanning Electron Microscopy showed changes on treated alfa surface which could promote the fibre-matrix adhesion. The reactivity of sumac tannins to formaldehyde test (Stiasny number) showed the possible use of sumac tannins in wood adhesive formulation. Thermomechanical analysis and strength analysis of sumac tannin/hexamin based resin highlighted acceptable bonding properties. The thermal conductivity measurement showed an average value equal to 0.110 W/m K. Contribution to the topical issue "Materials for Energy harvesting, conversion and storage II (ICOME 2016)", edited by Jean-Michel Nunzi, Rachid Bennacer and Mohammed El Ganaoui

A Patient Friendly Corifollitropin Alfa Protocol without Routine Pituitary Suppression in Normal Responders.

Science.gov (United States)

Wang, Huai-Ling; Lai, Hsing-Hua; Chuang, Tzu-Hsuan; Shih, Yu-Wei; Huang, Shih-Chieh; Lee, Meng-Ju; Chen, Shee-Uan

2016-01-01

The release of corifollitropin alfa simplifies daily injections of short-acting recombinant follicular stimulating hormone (rFSH), and its widely-used protocol involves short-acting gonadotropins supplements and a fixed GnRH antagonist regimen, largely based on follicle size. In this study, the feasibility of corifollitropin alfa without routine pituitary suppression was evaluated. A total of 288 patients were stimulated by corifollitropin alfa on cycle day 3 following with routine serum hormone monitoring and follicle scanning every other day after 5 days of initial stimulation, and a GnRH antagonist (0.25 mg) was only used prophylactically when the luteinizing hormone (LH) was ≧ 6 IU/L (over half of the definitive LH surge). The incidence of premature LH surge (≧ 10 IU/L) was 2.4% (7/288) before the timely injection of a single GnRH antagonist, and the elevated LH level was dropped down from 11.9 IU/L to 2.2 IU/L after the suppression. Two hundred fifty-one patients did not need any antagonist (87.2% [251/288]) throughout the whole stimulation. No adverse effects were observed regarding oocyte competency (fertilization rate: 78%; blastocyst formation rate: 64%). The live birth rate per OPU cycle after the first cryotransfer was 56.3% (161/286), and the cumulative live birth rate per OPU cycle after cyrotransfers was 69.6% (199/286). Of patients who did and did not receive GnRH antagonist during stimulation, no significant difference existed in the cumulative live birth rates (78.4% vs. 68.3%, p = 0.25). The results demonstrated that the routine GnRH antagonist administration is not required in the corifollitropin-alfa cycles using a flexible and hormone-depended antagonist regimen, while the clinical outcome is not compromised. This finding reveals that the use of a GnRH antagonist only occasionally may be needed.

A Patient Friendly Corifollitropin Alfa Protocol without Routine Pituitary Suppression in Normal Responders.

Directory of Open Access Journals (Sweden)

Huai-Ling Wang

Full Text Available The release of corifollitropin alfa simplifies daily injections of short-acting recombinant follicular stimulating hormone (rFSH, and its widely-used protocol involves short-acting gonadotropins supplements and a fixed GnRH antagonist regimen, largely based on follicle size. In this study, the feasibility of corifollitropin alfa without routine pituitary suppression was evaluated. A total of 288 patients were stimulated by corifollitropin alfa on cycle day 3 following with routine serum hormone monitoring and follicle scanning every other day after 5 days of initial stimulation, and a GnRH antagonist (0.25 mg was only used prophylactically when the luteinizing hormone (LH was ≧ 6 IU/L (over half of the definitive LH surge. The incidence of premature LH surge (≧ 10 IU/L was 2.4% (7/288 before the timely injection of a single GnRH antagonist, and the elevated LH level was dropped down from 11.9 IU/L to 2.2 IU/L after the suppression. Two hundred fifty-one patients did not need any antagonist (87.2% [251/288] throughout the whole stimulation. No adverse effects were observed regarding oocyte competency (fertilization rate: 78%; blastocyst formation rate: 64%. The live birth rate per OPU cycle after the first cryotransfer was 56.3% (161/286, and the cumulative live birth rate per OPU cycle after cyrotransfers was 69.6% (199/286. Of patients who did and did not receive GnRH antagonist during stimulation, no significant difference existed in the cumulative live birth rates (78.4% vs. 68.3%, p = 0.25. The results demonstrated that the routine GnRH antagonist administration is not required in the corifollitropin-alfa cycles using a flexible and hormone-depended antagonist regimen, while the clinical outcome is not compromised. This finding reveals that the use of a GnRH antagonist only occasionally may be needed.

Efficacy and safety of once-weekly intravenous epoetin alfa in maintaining hemoglobin levels in hemodialysis patients.

Science.gov (United States)

Locatelli, Francesco; Villa, Giuseppe; Messa, Piergiorgio; Filippini, Armando; Cannella, Giuseppe; De Ferrari, Giacomo; Naso, Agostino; Rossi, Egidio; Formica, Marco; Lombardi, Luigi; Rotolo, Ugo; Conte, Feruccio

2008-01-01

Although an erythropoiesis-stimulating agent (ESA) is most frequently administered intravenously for treatment of anemia in patients with chronic kidney disease who are on dialysis, few studies have compared the efficacy of different intravenous (i.v.) dosing schedules. This multicenter, phase IIIb, open-label, controlled study randomized 289 stable hemodialysis patients to continue with conventional dosing of i.v. epoetin alfa or darbepoetin, or to switch to once-weekly i.v. epoetin alfa at the same cumulative weekly starting dose, to maintain hemoglobin levels at 11.0-13.0 g/dL, and within 1.0 g/dL of the baseline value. Hemoglobin levels and ESA doses were recorded every 4 weeks for 28 weeks. Hemoglobin levels fell significantly and ESA doses increased significantly between baseline and week 28 (mean of week 16-28 values) in the once-weekly epoetin alfa group, compared with the conventional treatment group (phemoglobin levels between the groups was 0.73 g/dL (greater than the threshold for therapeutic equivalence of 0.5 g/dL). The changes between groups from baseline was significant at all time points for hemoglobin levels (0.36, 0.46, 0.81, 0.87, 0.78, 0.62 and 0.49 g/dL) and from week 12 for ESA dose (718.5, 1,326.5, 1,732.0, 1,839.7 and 1,959.1 IU/week; p=0.005). Hemoglobin was maintained at the target level in 78% and 84% of patients on conventional dosing, and 67% and 64% of those on once-weekly epoetin alfa in the intention-to-treat (p=0.1) and per protocol (p=0.016) populations, respectively. This study did not show therapeutic equivalence of once-weekly i.v. epoetin alfa with conventional dosing regimens.

LONG TERM MUCOLYTIC THERAPY WITH DORNASE ALFA AT NOVOSIBIRSK CENTER FOR DIAGNOSTICS, TREATMENT, RHEABILITATION AND ADAPTATION OF CHILDREN WITH CYSTIC FIBROSIS

Directory of Open Access Journals (Sweden)

N.I. Romanenko

2006-01-01

Full Text Available The center for diagnostic, treatment, rehabilitation and social adaptation of patients with cystic fibrosis was established in pulmonology department of the children's clinical hospital № 3 of Novosibirsk in 1994. Since 1995 the database of the center has registered 23 patients. the center has carried out an open prospective study of clinical efficacy and safety of dornase alfa (pulmozyme, «F. hoffmannala roche ltd.», basel in 10 patients with cystic fibrosis. The patients have received mucolytic therapy with dornase alfa for 2 years (2003–2005. They have been administered a daily inhalation of 2.5 ml of the solution (pulmozyme inhalation solution containing 2.5 of dornaza alfa. The study has shown an improvement of pulmonary function and elevation of oxygen saturation. The number of respiratory infections have dropped by 33%, the administration of intravenous antibiotics has shortened by 25%, the nutrition status has trended towards improvement. regardless of it's high price, dornasa alfa has proven its' benefits in the treatment of cystic fibrosis in patients of any age and disease severity.Key words: dornase alfa, children, cystic fibrosis, treatment.

Maintenance therapy with peginterferon alfa-2b does not prevent hepatocellular carcinoma in cirrhotic patients with chronic hepatitis C.

Science.gov (United States)

Bruix, Jordi; Poynard, Thierry; Colombo, Massimo; Schiff, Eugene; Burak, Kelly; Heathcote, Elizabeth J L; Berg, Thomas; Poo, Jorge-Luis; Mello, Carlos Brandao; Guenther, Rainer; Niederau, Claus; Terg, Ruben; Bedossa, Pierre; Boparai, Navdeep; Griffel, Louis H; Burroughs, Margaret; Brass, Clifford A; Albrecht, Janice K

2011-06-01

Several studies have reported that low doses of interferon can delay the development of hepatocellular carcinoma (HCC) and progression of chronic hepatitis C. We investigated the incidence of clinical events among participants of the Evaluation of PegIntron in Control of Hepatitis C Cirrhosis (EPIC)3 program. Data were analyzed from an open-label randomized study of patients with chronic hepatitis C who had failed to respond to interferon alfa plus ribavirin. All patients had compensated cirrhosis with no evidence of HCC. Patients received peginterferon alfa-2b (0.5 μg/kg/week; n=311) or no treatment (controls, n=315) for a maximum period of 5 years or until 98 patients had a clinical event (hepatic decompensation, HCC, death, or liver transplantation). The primary measure of efficacy was time until the first clinical event. There was no significant difference in time to first clinical event among patients who received peginterferon alfa-2b compared with controls (hazard ratio [HR], 1.452; 95% confidence interval [CI]: 0.880-2.396). There was no decrease in the development of HCC with therapy. The time to disease progression (clinical events or new or enlarged varices) was significantly longer for patients who received peginterferon alfa-2b compared with controls (HR, 1.564; 95% CI: 1.130-2.166). In a prospectively defined subanalysis of patients with baseline portal hypertension, peginterferon alfa-2b significantly increased the time to first clinical event compared with controls (P=.016). There were no new safety observations. Maintenance therapy with peginterferon alfa-2b is not warranted in all patients and does not prevent HCC. However, there is a potential clinical benefit of long-term suppressive therapy in patients with preexisting portal hypertension. Copyright © 2011 AGA Institute. Published by Elsevier Inc. All rights reserved.

GSI Helmholz Centre for Heavy Ion Research - ALFA: Next generation concurrent framework for ALICE and FAIR experiments

CERN Multimedia

CERN. Geneva

2015-01-01

FAIR is a new, unique international accelerator facility for the research with antiprotons and ions. It is being built at the GSI Darmstadt in Hesse, Germany. The commonalities between the ALICE and FAIR experiments and their computing requirements led to the development of a common software framework in an experiment independent way; ALFA (ALICE-FAIR framework). ALFA is designed for high quality parallel data processing and reconstruction on heterogeneous computing systems. It provides a data transport layer and the capability to coordinate multiple data processing components. ALFA is a flexible, elastic system which balances reliability and ease of development with performance by using a message based multi-processing in addition to multi-threading. The framework allows for usage of heterogeneous computing architectures by offloading (portions of code are accelerated on the device) or natively (where the full program is executed on the device ).

Andexanet Alfa for Acute Major Bleeding Associated with Factor Xa Inhibitors

NARCIS (Netherlands)

Connolly, Stuart J.; Milling, Truman J.; Eikelboom, John W.; Gibson, C. Michael; Curnutte, John T.; Gold, Alex; Bronson, Michele D.; Lu, Genmin; Conley, Pamela B.; Verhamme, Peter; Schmidt, Jeannot; Middeldorp, Saskia; Cohen, Alexander T.; Beyer-Westendorf, Jan; Albaladejo, Pierre; Lopez-Sendon, Jose; Goodman, Shelly; Leeds, Janet; Wiens, Brian L.; Siegal, Deborah M.; Zotova, Elena; Meeks, Brandi; Nakamya, Juliet; Lim, W. Ting; Crowther, Mark; Connolly, S. C.; Crowther, M.; Eikelboom, J.; Gibson, M.; Milling, T. J.; Albaladejo, P.; Cohen, A.; Lopez-Sendon, J.; Schmidt, J.; Verhamme, P.; Beyer-Westendorf, J.; Wyse, D. G.; Garcia, D.; Prins, M. [=Martin H.; Nakamya, J.; Büller, H. R.; Mahaffey, K.; Alexander, J.; Demchuk, A.; Raskob, G.; Schulman, S.; Meeks, B.; Zotova, E.; Holadyk-Gris, I.; Coppens, M.

2016-01-01

Background Andexanet alfa (andexanet) is a recombinant modified human factor Xa decoy protein that has been shown to reverse the inhibition of factor Xa in healthy volunteers. Methods In this multicenter, prospective, open-label, single-group study, we evaluated 67 patients who had acute major

Evaluation of pharmacokinetics, user handling, and tolerability of peginterferon alfa-2a (40 kDa) delivered via a disposable autoinjector device

Science.gov (United States)

Varunok, Peter; Lawitz, Eric; Beavers, Kimberly L; Matusow, Gary; Leong, Ruby; Lambert, Nathalie; Bernaards, Coen; Solsky, Jonathan; Brennan, Barbara J; Wat, Cynthia; Bertasso, Anne

2011-01-01

Background Peginterferon alfa-2a (40 kDa) is currently administered using a prefilled syringe. The peginterferon alfa-2a disposable autoinjector is a new safety-engineered device designed to facilitate injection and reduce the risk of needlestick injuries. The analysis of two open-label Phase I trials evaluated the pharmacokinetics, successful administration, and tolerability of peginterferon alfa-2a when using the autoinjector. The studies were performed to support the filing and registration of the autoinjector device. Methods In trial 1, 50 healthy adult subjects received one 180 μg dose of peginterferon alfa-2a via the autoinjector. Serial blood samples were collected predose, up to 336 hours following drug administration, and at follow-up (28 ± 3 days post-dosing) for noncompartmental pharmacokinetic analysis. Trial 2 randomized 60 adult patients with chronic hepatitis C to 180 μg peginterferon alfa-2a once weekly by the autoinjector or prefilled syringe for 3 weeks followed by the alternative device (prefilled syringe or autoinjector, respectively) for 3 weeks. Patients also received ribavirin. Administration by the devices was evaluated under direct observation by a study staff member and by patient subjective assessment. Results In trial 1, following a single dose of peginterferon alfa-2a, the maximum plasma concentration was 16.1 ± 5.3 ng/mL (mean ± standard deviation), and area under the concentration time curve (0–168 hours) was 1996 ± 613 ng · hour/mL, similar to that reported using a vial/syringe or prefilled syringe. In trial 2, few patients showed handling difficulties with either device. Generally, patients were observed to be more satisfied and confident, followed instructions better, and successfully initiated injection with the autoinjector versus the prefilled syringe. Patients reported the autoinjector to be more convenient and easier to use. No pain or discomfort was experienced using the autoinjector. The autoinjector safety profile

Is dibotermin alfa a cost-effective substitute for autologous iliac crest bone graft in single level lumbar interbody spine fusion?

Science.gov (United States)

Svedbom, Axel; Paech, Daniel; Leonard, Catherine; Donnell, David; Song, Fujian; Boszcyk, Bronek; Rothenfluh, Dominique A; Lloyd, Andrew; Borgman, Benny

2015-11-01

To evaluate the cost-effectiveness of dibotermin alfa compared with autologous iliac crest bone graft (ICBG) for patients undergoing single level lumbar interbody spinal fusion in a UK hospital setting. An individual patient data (IPD) meta-analysis of six randomized controlled clinical trials and two single arm trials compared dibotermin alfa on an absorbable collagen implantation matrix (ACIM) (n = 456) and ICBG (n = 244) on resource use, re-operation rates, and SF-6D (Short form 6-dimension) health utility (total N = 700). Failure-related second surgery, operating time, post-operative hospital stay, and quality-adjusted life years (QALYs) derived from the IPD meta-analysis were included as inputs in an economic evaluation undertaken to assess the cost-effectiveness of dibotermin alfa/ACIM versus ICBG for patients undergoing single level lumbar interbody spinal fusion. A four year time horizon and the United Kingdom (UK) National Health Service (NHS) and Personal Social Services (PSS) perspective was adopted in the base case, with sensitivity analyses performed to gauge parameter uncertainty. In the base case analysis, patients treated using dibotermin alfa/ACIM (12 mg pack) accrued 0.055 incremental QALYs at an incremental cost of £ 737, compared with patients treated with ICBG. This resulted in an incremental cost-effectiveness ratio (ICER) of £ 13,523, indicating that at a willingness-to-pay threshold of £ 20,000, dibotermin alfa/ACIM is a cost-effective intervention relative to ICBG from the NHS and PSS perspective. In a UK hospital setting, dibotermin alfa/ACIM is a cost-effective substitute for ICBG for patients who require lumbar interbody arthrodesis.

Pathophysiology of hypophosphatasia and the potential role of asfotase alfa.

Science.gov (United States)

Orimo, Hideo

2016-01-01

Hypophosphatasia (HPP) is an inherited systemic bone disease that is characterized by bone hypomineralization. HPP is classified into six forms according to the age of onset and severity as perinatal (lethal), perinatal benign, infantile, childhood, adult, and odontohypophosphatasia. The causative gene of the disease is the ALPL gene that encodes tissue-nonspecific alkaline phosphatase (TNAP). TNAP is expressed ubiquitously, and its physiological role is apparent in bone mineralization. A defect in bone mineralization can manifest in several ways, including rickets or osteomalacia in HPP patients. Patients with severe forms suffer from respiratory failure because of hypoplastic chest, which is the main cause of death. They sometimes present with seizures due to a defect in vitamin B6 metabolism resulting from the lack of alkaline phosphatase activity in neuronal cells, which is also lethal. Patients with a mild form of the disease exhibit rickets or osteomalacia and a functional defect of exercise. Odontohypophosphatasia shows only dental manifestations. To date, 302 mutations in the ALPL gene have been reported, mainly single-nucleotide substitutions, and the relationships between phenotype and genotype have been partially elucidated. An established treatment for HPP was not available until the recent development of enzyme replacement therapy. The first successful enzyme replacement therapy in model mice using a modified human TNAP protein (asfotase alfa) was reported in 2008, and subsequently success in patients with severe form of the disease was reported in 2012. In 2015, asfotase alfa was approved in Japan in July, followed by in the EU and Canada in August, and then by the US Food and Drug Administration in the USA in October. It is expected that therapy with asfotase alfa will drastically change treatments and prognosis of HPP.

Arecibo pulsar survey using ALFA: probing radio pulsar intermittency and transients

NARCIS (Netherlands)

Deneva, J.S.; Cordes, J.M.; McLaughlin, M.A.; Nice, D.J.; Lorimer, D.R.; Crawford, F.; Bhat, N.D.R.; Camilo, F.; Champion, D.J.; Freire, P.C.C.; Edel, S.; Kondratiev, V.I.; Hessels, J.W.T.; Jenet, F.A.; Kasian, L.; Kaspi, V.M.; Kramer, M.; Lazarus, P.; Ransom, S.M.; Stairs, I.H.; Stappers, B.W.; van Leeuwen, J.; Brazier, A.; Venkataraman, A.; Zollweg, J.A.; Bogdanov, S.

2009-01-01

We present radio transient search algorithms, results, and statistics from the ongoing Arecibo Pulsar ALFA (PALFA) survey of the Galactic plane. We have discovered seven objects through a search for isolated dispersed pulses. All of these objects are Galactic and have measured periods between 0.4

Towards a Total Cross Section Measurement with the ALFA Detector at ATLAS

CERN Document Server

Trzebiński, Maciej

2013-01-01

The main goals of the Absolute Luminosity For ATLAS (ALFA) detector is to provide an absolute luminosity and total cross section measurement. The measurement method used, the detector alignment and the quality of the collected data are discussed.

Pegylated interferons Lambda-1a and alfa-2a display different gene induction and cytokine and chemokine release profiles in whole blood, human hepatocytes and peripheral blood mononuclear cells.

Science.gov (United States)

Freeman, J; Baglino, S; Friborg, J; Kraft, Z; Gray, T; Hill, M; McPhee, F; Hillson, J; Lopez-Talavera, J C; Wind-Rotolo, M

2014-06-01

Pegylated interferon-lambda-1a (Lambda), a type III interferon (IFN) in clinical development for the treatment of chronic HCV infection, has shown comparable efficacy and an improved safety profile to a regimen based on pegylated IFN alfa-2a (alfa). To establish a mechanistic context for this improved profile, we investigated the ex vivo effects of Lambda and alfa on cytokine and chemokine release, and on expression of IFN-stimulated genes (ISGs) in primary human hepatocytes and peripheral blood mononuclear cells (PBMCs) from healthy subjects. Our findings were further compared with changes observed in blood analysed from HCV-infected patients treated with Lambda or alfa in clinical studies. mRNA transcript and protein expression of the IFN-λ-limiting receptor subunit was lower compared with IFN-α receptor subunits in all cell types. Upon stimulation, alfa and Lambda induced ISG expression in hepatocytes and PBMCs, although in PBMCs Lambda-induced ISG expression was modest. Furthermore, alfa and Lambda induced release of cytokines and chemokines from hepatocytes and PBMCs, although differences in their kinetics of induction were observed. In HCV-infected patients, alfa treatment induced ISG expression in whole blood after single and repeat dosing. Lambda treatment induced modest ISG expression after single dosing and showed no induction after repeat dosing. Alfa and Lambda treatment increased IP-10, iTAC, IL-6, MCP-1 and MIP-1β levels in serum, with alfa inducing higher levels of all mediators compared with Lambda. Overall, ex vivo and in vivo induction profiles reported in this analysis strongly correlate with clinical observations of fewer related adverse events for Lambda vs those typically associated with alfa. © 2014 John Wiley & Sons Ltd.

Human factors engineering and design validation for the redesigned follitropin alfa pen injection device.

Science.gov (United States)

Mahony, Mary C; Patterson, Patricia; Hayward, Brooke; North, Robert; Green, Dawne

2015-05-01

To demonstrate, using human factors engineering (HFE), that a redesigned, pre-filled, ready-to-use, pre-asembled follitropin alfa pen can be used to administer prescribed follitropin alfa doses safely and accurately. A failure modes and effects analysis identified hazards and harms potentially caused by use errors; risk-control measures were implemented to ensure acceptable device use risk management. Participants were women with infertility, their significant others, and fertility nurse (FN) professionals. Preliminary testing included 'Instructions for Use' (IFU) and pre-validation studies. Validation studies used simulated injections in a representative use environment; participants received prior training on pen use. User performance in preliminary testing led to IFU revisions and a change to outer needle cap design to mitigate needle stick potential. In the first validation study (49 users, 343 simulated injections), in the FN group, one observed critical use error resulted in a device design modification and another in an IFU change. A second validation study tested the mitigation strategies; previously reported use errors were not repeated. Through an iterative process involving a series of studies, modifications were made to the pen design and IFU. Simulated-use testing demonstrated that the redesigned pen can be used to administer follitropin alfa effectively and safely.

Preparation And Properties Of Bionanocomposite Films Reinforced With Nanocellulose Isolated From Moroccan Alfa Fibres

Directory of Open Access Journals (Sweden)

Youssef Benyoussif

2015-09-01

Full Text Available Nanocellulose (NC were extracted from the Moroccan Alfa plant (Stipa tenacissima L. and characterised. These Alfa cellulosic nanoparticles were used as reinforcing phase to prepare bionanocomposite films using carboxymethyl cellulose as matrix. These films were obtained by the casting/evaporation method. The crystallinity of NC was analysed by X-ray diffraction, the dimension of NC by atomic force microscopy, molecular interactions due to incorporation of NC in carboxymethyl cellulose (CMC matrix were supported by Fourier transforms infrared (FTIR spectroscopy. The properties of the ensuing bionanocomposite films were investigated using tensile tests, water vapour permeability (WVP study and thermogravimetric analysis. With the progress of purification treatment of cellulose, the crystallinity is improved compared to the untreated fibres; this can be explained by the disappearance of the amorphous areas in cellulose chain of the plant. Consequently, the tensile modulus and tensile strength of CMC film increased by 60 and 47%, respectively, in the bionanocomposite films with 10 wt% of NC, and decrease by 8.6% for WVP with the same content of NC. The NC obtained from the Moroccan Alfa fibres can be used as a reinforcing agent for the preparation of bionanocomposites, and they have a high potential for the development of completely biodegradable food packaging materials.

Alfa-Laval plate heat exchangers for the power industries

International Nuclear Information System (INIS)

Kitae, Junnosuke; Mtsuura, Kazuyuki

1979-01-01

Within power-generating plants, the transfer and conversion of heat energy of very large quantity are carried out in the process of energy conversion, accordingly the importance of heat exchangers is very high. Heretofore, multi-tube heat exchangers have been used mostly, but Alfa-Laval group developed the heat exchanger with very high efficiency to incorporate it effectively into a power-generating plant. In this plate type heat exchanger, the heat transfer efficiency is very high, and the quantity of stagnation is small as it is compact, consequently it is suitable to the secondary cooling for power-generating plant or the heat exchange of high-priced liquid heat media such as heavy water. Originally, plate type heat exchangers were used for food and chemical industries, therefore the prevention of mixing two liquids, sanitary construction, and corrosion resistance were required. Then they were adopted in iron and steel industry, and large thermal load, large heat transfer area and corrosion resistance to sea water were required. They were adopted in a nuclear power plant for the first time in 1964. In this heat exchanger, channels are formed with corrugated metal sheets, and titanium, stainless steels, Incoloy, Hastelloy and others are used as occasion demands. The Alfa-Laval heat exchangers and their features are explained. (Kako, I.)

Patient evaluation of the use of follitropin alfa in a prefilled ready-to-use injection pen in assisted reproductive technology: an observational study

Directory of Open Access Journals (Sweden)

Welcker J

2010-09-01

Full Text Available Abstract Background Self-administration of recombinant human follicle-stimulating hormone (r-hFSH can be performed using injection pen devices by women undergoing assisted reproductive technology procedures. The objective of this study was to explore the use of the prefilled follitropin alfa pen in routine assisted reproductive technology procedures in Germany. Methods This prospective, observational study was conducted across 43 German IVF centres over a period of 1.75 years. Patients who had used the prefilled follitropin alfa pen in the current or a previous cycle of controlled ovarian stimulation completed a questionnaire to assess their opinions of the device. Results A total of 5328 patients were included in the study. Of these, 2888 reported that they had previous experience of daily FSH injections. Significantly more patients reported that less training was required to use the prefilled follitropin alfa pen than a syringe and lyophilized powder (1997/3081 [64.8%]; p Conclusions In this questionnaire-based survey, routine use of the prefilled follitropin alfa pen was well accepted and associated with favourable patient perceptions. Users of the pen found it easier to initially learn how to use, and subsequently use, than other injection methods. In general, the prefilled follitropin alfa pen was the preferred method for self-administration of gonadotrophins. Together with previous findings, the results here indicate a high level of patient satisfaction among users of the prefilled follitropin alfa pen for daily self-administration of r-hFSH.

Corifollitropin alfa followed by rFSH in a GnRH antagonist protocol for poor ovarian responder patients

DEFF Research Database (Denmark)

Polyzos, Nikolaos P; Devos, Michel; Humaidan, Peter

2013-01-01

OBJECTIVE: To identify whether women with poor ovarian response may benefit from treatment with corifollitropin alfa in a GnRH antagonist protocol. DESIGN: Retrospective pilot study. SETTING: University-based tertiary care center. PATIENT(S): Poor ovarian responders fulfilling the Bologna criteria...... developed by European Society for Human Reproduction and Embryology Consensus Group. INTERVENTION(S): Corifollitropin alfa (150 μg) followed by 300 IU rFSH in a GnRH antagonist protocol. MAIN OUTCOME MEASURE(S): Endocrinologic profile and ongoing pregnancy rates. RESULT(S): Among 43 women treated...

alfa-Deuterium kinetic isotope effects in reactions of methyllithium. Is better aggregation the cause of lower reactivity?

DEFF Research Database (Denmark)

Holm, Torkil

1996-01-01

The value of kH/kD for alfa deuterium kinetic isotope effects for the reaction of methyllithium and methylmagnesium iodid with a series of substrates are consistently ca. 10-15 % higher for the lithium reagent. This may indicate a pre-equilibrium......The value of kH/kD for alfa deuterium kinetic isotope effects for the reaction of methyllithium and methylmagnesium iodid with a series of substrates are consistently ca. 10-15 % higher for the lithium reagent. This may indicate a pre-equilibrium...

The G209A mutation in the alpha-synuclein gene in Brazilian families with Parkinson's disease Mutação G209A no gene da alfa-sinucleína em famílias brasileiras com doença de Parkinson

Directory of Open Access Journals (Sweden)

Hélio A.G. Teive

2001-09-01

Full Text Available A missense G209A mutation of the alpha-synuclein gene was recently described in a large Contursi kindred with Parkinson's disease (PD. The objective of this study is to determine if the mutation G209A of the alpha-synuclein gene was present in 10 Brazilian families with PD. PD patients were recruited from movement disorders clinics of Brazil. A family history with two or more affected in relatives was the inclusion criterion for this study. The alpha-synuclein G209A mutation assay was made using polymerase chain reaction and the restriction enzyme Tsp45I. Ten patients from 10 unrelated families were studied. The mean age of PD onset was 42.7 years old. We did not find the G209A mutation in our 10 families with PD. Our results suggest that alpha-synuclein mutation G209A is uncommon in Brazilian PD families.Recentemente foi detectada mutação missense G209A no gene da alfa-sinucleína em uma grande família com doença de Parkinson (DP de Contursi, Itália. Este estudo tem o objetivo de determinar se a mutação G209A está presente em 10 famílias brasileiras com DP. Pacientes com DP foram recrutados em clínicas de distúrbio do movimento no Brasil. O critério de inclusão no estudo foi à presença de dois ou mais familiares acometidos pela DP. A mutação G209A do gene da alfa-sinucleína foi pesquisada usando a técnica de reação em cadeia de polimerase e a enzima de restrição Tsp45I. Foram estudados 10 pacientes de famílias não-relacionadas. A idade média do início dos sintomas da DP foi 42,7 anos. Não encontramos a mutação estudada neste grupo de pacientes. Nossos resultados sugerem que a mutação G209A é incomum em famílias brasileiras com DP.

Evaluation of pharmacokinetics, user handling, and tolerability of peginterferon alfa-2a (40 kDa delivered via a disposable autoinjector device

Directory of Open Access Journals (Sweden)

Varunok P

2011-11-01

Full Text Available Peter Varunok1, Eric Lawitz2, Kimberly L Beavers3, Gary Matusow4, Ruby Leong5, Nathalie Lambert6, Coen Bernaards7, Jonathan Solsky5, Barbara J Brennan5, Cynthia Wat8, Anne Bertasso51Gastroenterology Associates, Poughkeepsie, NY, USA; 2Alamo Medical Research, San Antonio, TX, USA; 3Asheville Gastroenterology, Asheville, NC, USA; 4Gastroenterology Group, South Jersey, NJ, USA; 5Roche, Nutley, NJ, USA; 6Roche, Basel, Switzerland; 7Roche, San Francisco, CA, USA; 8Roche, Welwyn, UKBackground: Peginterferon alfa-2a (40 kDa is currently administered using a prefilled syringe. The peginterferon alfa-2a disposable autoinjector is a new safety-engineered device designed to facilitate injection and reduce the risk of needlestick injuries. The analysis of two open-label Phase I trials evaluated the pharmacokinetics, successful administration, and tolerability of peginterferon alfa-2a when using the autoinjector. The studies were performed to support the filing and registration of the autoinjector device.Methods: In trial 1, 50 healthy adult subjects received one 180 µg dose of peginterferon alfa-2a via the autoinjector. Serial blood samples were collected predose, up to 336 hours following drug administration, and at follow-up (28 ± 3 days post-dosing for noncompartmental pharmacokinetic analysis. Trial 2 randomized 60 adult patients with chronic hepatitis C to 180 µg peginterferon alfa-2a once weekly by the autoinjector or prefilled syringe for 3 weeks followed by the alternative device (prefilled syringe or autoinjector, respectively for 3 weeks. Patients also received ribavirin. Administration by the devices was evaluated under direct observation by a study staff member and by patient subjective assessment.Results: In trial 1, following a single dose of peginterferon alfa-2a, the maximum plasma concentration was 16.1 ± 5.3 ng/mL (mean ± standard deviation, and area under the concentration time curve (0–168 hours was 1996 ± 613 ng · hour

Accurate Learning with Few Atlases (ALFA): an algorithm for MRI neonatal brain extraction and comparison with 11 publicly available methods.

Science.gov (United States)

Serag, Ahmed; Blesa, Manuel; Moore, Emma J; Pataky, Rozalia; Sparrow, Sarah A; Wilkinson, A G; Macnaught, Gillian; Semple, Scott I; Boardman, James P

2016-03-24

Accurate whole-brain segmentation, or brain extraction, of magnetic resonance imaging (MRI) is a critical first step in most neuroimage analysis pipelines. The majority of brain extraction algorithms have been developed and evaluated for adult data and their validity for neonatal brain extraction, which presents age-specific challenges for this task, has not been established. We developed a novel method for brain extraction of multi-modal neonatal brain MR images, named ALFA (Accurate Learning with Few Atlases). The method uses a new sparsity-based atlas selection strategy that requires a very limited number of atlases 'uniformly' distributed in the low-dimensional data space, combined with a machine learning based label fusion technique. The performance of the method for brain extraction from multi-modal data of 50 newborns is evaluated and compared with results obtained using eleven publicly available brain extraction methods. ALFA outperformed the eleven compared methods providing robust and accurate brain extraction results across different modalities. As ALFA can learn from partially labelled datasets, it can be used to segment large-scale datasets efficiently. ALFA could also be applied to other imaging modalities and other stages across the life course.

Mango resistance to fruit flies. II - resistance of the alfa cultivar

Energy Technology Data Exchange (ETDEWEB)

Rossetto, C.J.; Bortoletto, N., E-mail: rossetto@iac.sp.gov.b [Agencia Paulista de Tecnologia dos Agronegocios (APTA), Votuporanga, SP (Brazil). Polo Regional do Noroeste Paulista; Walder, J.M.M.; Mastrangelo, T. de A., E-mail: jmwalder@cena.usp.b [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Carvalho, C.R.L.; Castro, J.V. de, E-mail: climonta@iac.sp.gov.b, E-mail: josalba@iac.sp.gov.b [Instituto Agronomico de Campinas, SP (Brazil); Pinto, A.C. de Q. [EMBRAPA, Brasilia, DF (Brazil); Cortelazzo, A.L., E-mail: angelo@unicamp.b [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Inst. de Biologia

2006-07-01

The percentage of infested mango fruits of five selected mango varieties was evaluated during three years under field conditions. Three varieties with field resistance to fruit flies had less then 10% of fruits infested. Tommy Atkins, the susceptible commercial check, had 42,9% and the susceptible check had 98.9 % of infested fruits. The three field resistant varieties plus the susceptible commercial check, Tommy Atkins, were further tested in laboratory, under caged conditions, with artificial infestation of Anastrepha obliqua. The attempts of oviposition and the number of pupae developed from each fruit were evaluated. Under caged conditions, the cultivar Alfa maintained its field resistance and Espada Stahl and IAC 111 lost the field resistance and were as susceptible as Tommy Atkins. The attempts of oviposition were positively and highly correlated with the number of pupae developed in the fruits. Non preference for oviposition was confirmed as the main mechanism of resistance of mango fruits to fruit flies. In the absence of a more susceptible variety (no choice test) the cultivar Alfa has kept the resistance (author)

Mango resistance to fruit flies. II - resistance of the alfa cultivar

International Nuclear Information System (INIS)

Rossetto, C.J.; Bortoletto, N.; Carvalho, C.R.L.; Castro, J.V. de; Pinto, A.C. de Q.; Cortelazzo, A.L.

2006-01-01

The percentage of infested mango fruits of five selected mango varieties was evaluated during three years under field conditions. Three varieties with field resistance to fruit flies had less then 10% of fruits infested. Tommy Atkins, the susceptible commercial check, had 42,9% and the susceptible check had 98.9 % of infested fruits. The three field resistant varieties plus the susceptible commercial check, Tommy Atkins, were further tested in laboratory, under caged conditions, with artificial infestation of Anastrepha obliqua. The attempts of oviposition and the number of pupae developed from each fruit were evaluated. Under caged conditions, the cultivar Alfa maintained its field resistance and Espada Stahl and IAC 111 lost the field resistance and were as susceptible as Tommy Atkins. The attempts of oviposition were positively and highly correlated with the number of pupae developed in the fruits. Non preference for oviposition was confirmed as the main mechanism of resistance of mango fruits to fruit flies. In the absence of a more susceptible variety (no choice test) the cultivar Alfa has kept the resistance (author)

Pancreatitis induced by pegylated interferon alfa-2b in a patient affected by chronic hepatitis C.

Science.gov (United States)

Cecchi, Enrica; Forte, Paolo; Cini, Elisabetta; Banchelli, Grazia; Ferlito, Chiara; Mugelli, Alessandro

2004-01-01

A middle-aged man was admitted to the ED because of nausea and vomiting, abdominal distention and fainting. A blood analysis revealed high levels of serum amylase and lipase, confirming a diagnosis of acute pancreatitis. The history showed that the patient had self-administered a single dose of pegylated interferon alfa-2b and ribavirin daily for 7 days for chronic hepatitis C. The medications were stopped and his condition gradually improved. In agreement with the literature and the Naranjo algorythm result, pegylated interferon alfa-2b is associated with acute pancreatitis. Identification of a few signs and symptoms is the first 'signal' in preventing a serious drug-induced adverse event.

Elosulfase alfa enzyme replacement therapy attenuates disease progression in a non-ambulatory Japanese patient with Morquio A syndrome (case report

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Misako Hiramatsu

2017-12-01

Full Text Available Enzyme replacement therapy (ERT with elosulfase alfa is the only approved therapy in Japan for patients with Morquio A syndrome, a lysosomal storage disorder inherited in an autosomal recessive fashion. The experience with ERT in severely affected, non-ambulatory patients has not been reported in previous studies. This case report describes clinical evidence for the 1-year efficacy and safety of ERT with elosulfase alfa in a severely affected, non-ambulatory, 47-year-old patient with Morquio A syndrome who needs intensive respiratory management. ERT with elosulfase alfa was well tolerated in this patient. Because of the possibility of potential hypersensitivity adverse events, special attention is needed when using ERT in patients with respiratory disorders. However, under the appropriate management of specialists, the patient in this case report showed significant respiratory improvement after starting ERT, and abdominal bloating was improved by gas evacuation. In addition, the patient was able to lift up her arms, reach behind her back, and move her legs slightly, and she recovered her grip strength. Her hearing loss improved and she could hear without a hearing aid. This report shows that ERT with elosulfase alfa can be used with appropriate respiratory care in patients with severe respiratory dysfunction.

An open-label clinical trial to investigate the efficacy and safety of corifollitropin alfa combined with hCG in adult men with hypogonadotropic hypogonadism.

Science.gov (United States)

Nieschlag, Eberhard; Bouloux, Pierre-Marc G; Stegmann, Barbara J; Shankar, R Ravi; Guan, Yanfen; Tzontcheva, Anjela; McCrary Sisk, Christine; Behre, Hermann M

2017-03-07

Hypogonadotropic hypogonadism (HH) in men results in insufficient testicular function and deficiencies in testosterone and spermatogenesis. Combinations of human chorionic gonadotropin (hCG) and recombinant follicle-stimulating hormone (recFSH) have been successful in the treatment of HH. Corifollitropin alfa is a long-acting FSH-analog with demonstrated action in women seeking infertility care. The aim of this study was to investigate the efficacy and safety of corifollitropin alfa combined with hCG to increase testicular volume and induce spermatogenesis in men with HH. This was a Phase III, multi-center, open-label, single-arm trial of corifollitropin alfa in azoospermic men aged 18 to 50 years with HH. After 16 weeks of pretreatment of 23 subjects with hCG alone, 18 subjects with normalized testosterone (T) levels who remained azoospermic entered the 52-week combined treatment phase with hCG twice-weekly and 150 μg corifollitropin alfa every other week. The increase in testicular volume (primary efficacy endpoint) and induction of spermatogenesis resulting in a sperm count ≥1 × 10 6 /mL (key secondary efficacy endpoint) during 52 weeks of combined treatment were assessed. Safety was evaluated by the presence of anti-corifollitropin alfa antibodies and the occurrence of adverse events (AEs). Mean (±SD) testicular volume increased from 8.6 (±6.09) mL to 17.8 (±8.93) mL (geometric mean fold increase, 2.30 [95% CI: 2.03, 2.62]); 14 (77.8%) subjects reached a sperm count ≥1 × 10 6 /mL. No subject developed confirmed anti-corifollitropin alfa antibodies during the trial. Treatment was generally well tolerated. Corifollitropin alfa 150 μg administrated every other week combined with twice-weekly hCG for 52 weeks increased testicular volume significantly, and induced spermatogenesis in >75% of men with HH who had remained azoospermic after hCG treatment alone. ClinicalTrials.gov: NCT01709331 .

Feasibility of corifollitropin alfa/GnRH antagonist protocol combined with GnRH agonist triggering and freeze-all strategy in polycystic ovary syndrome patients

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Jiann-Loung Hwang

2018-06-01

Full Text Available Background/Purpose: The long-acting corifollitropin alfa is comparable to FSH in terms of pregnancy outcomes in normal responders and poor responders. Corifollitropin alfa has never been studied in polycystic ovary syndrome (PCOS patients because of concerns of excessive ovarian stimulation and ovarian hyperstimulation syndrome (OHSS. The purpose of the study was to evaluate if corifollitropin alfa can be used in PCOS patients. Methods: Forty PCOS patients who were going to undergo in vitro fertilization were enrolled in this study. A single injection of corifollitropin alfa was administered on cycle day 2 or day 3. From stimulation day 8 onwards, daily FSH was administered until the day of final oocyte maturation. Cetrorelix was administered from stimulation day 5 to prevent premature LH surge. Final oocyte maturation was triggered by: acetate. All embryos were cryopreserved and replaced in subsequent cycles. Results: All 40 patients were subjected to oocyte retrieval, and none developed moderate or severe ovarian hyperstimulation syndrome (0%, 95% CI 0–0.088. For each patient, an average of 23.4 (±7.4; 95% CI 21.0–25.7 oocytes were retrieved and a mean of 11.7 (±6.4; 95% CI 9.6–13.8 embryos were frozen. Mean serum estradiol level on the day of GnRHa triggering was 7829.9 pg/ml (±3297; 95% CI 6775–8885. The cumulated ongoing pregnancy rate after 3 frozen-thawed embryo transfers was 75.0% (95% CI 61.6%–88.4%. Conclusion: The results suggest that corifollitropin alfa/GnRH antagonist protocol can be used in PCOS patients, in combination with GnRHa triggering and embryo cryopreservation. Keywords: Corifollitropin alfa, Cryopreservation, GnRH agonist, Polycystic ovary syndrome

ALFA detector, Background removal and analysis for elastic events

CERN Document Server

Belaloui, Nazim

2017-01-01

I worked on the ALFA project, which has the aim to measure the total cross section in PP collisions as a function of t, the momentum transfer by measuring the scattering angle of the protons. This measurement is done for all available energies; so far 7, 8 and 13 TeV. There are many analysis steps and we have focused on enhancing the signal-to-noise ratio. First of all I tried to be more familiar with ROOT, worked on understanding the code used to access to the data, plotting histograms, then cutting-off background.

Production and properties of alpha-amylase from thermophilic Bacillus sp. Produção e propriedades de alfa-amilase de Bacillus sp. termofílico

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Carlos Alberto Martins Cordeiro

2002-01-01

Full Text Available alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1 production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid media containing soluble starch reached a maximum at 48h, with levels of 57U/mL. Studies on the a-amylase characterization revealed that the optimum temperature for activity was 70ºC. The enzyme was stable for 2h at 50ºC, while at 60ºC, 70ºC and 90ºC, 4%, 13% and 38% of the original activities were lost, respectively. The optimum pH of the enzyme was 7.5. After incubation of crude enzyme solution for 24h at pH 7.5, a decrease of about 5% of its original activity was observed. The enzyme was strongly inhibited by Co2+, Cu2+ and Ba2+, but less affected by Ca2+, Mg2+, Ni2+, Sr2+ and Mn2+. The enzyme in 1M and 5M NaCl solutions the enzyme retained 70% and 47% of the original activity after 24h of incubation at 4ºC, respectively.A produção de alfa-amilase (1,4-alfa-D-glicano glicanohidrolase, EC 3.2.1.1 por um Bacillus sp cepa SMIA-2 cultivado em meios líquidos contendo amido solúvel, alcançou o máximo em 48h com níveis de 57U/mL. Estudos sobre a caracterização de alfa-amilase revelaram que a temperatura ótima de atividade desta enzima foi 70ºC. A enzima foi estável por 2h a 50ºC, enquanto que a 60ºC, 70ºC e 90ºC, 4%, 13% e 38% da atividade original foram perdidas, respectivamente. O pH ótimo da enzima foi 7,5. Após a incubação da enzima bruta por 24h a pH 7,5 observou-se um decréscimo em torno de 5% de sua atividade original. A enzima foi fortemente inibida por Co2+, Cu2+ e Ba2+, mas foi menos afetada por Ca2+, Mg2+, Ni2+, Sr2+ e Mn2+. Em solução de NaCl 1M e 5M, a enzima reteve 70% e 47% da sua atividade original após 24h a 4ºC, respectivamente.

The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein

Czech Academy of Sciences Publication Activity Database

Vošahlíková, Miroslava; Svoboda, Petr

2011-01-01

Roč. 60, č. 3 (2011), s. 541-547 ISSN 0862-8408 R&D Projects: GA AV ČR(CZ) IAA500110606; GA MŠk(CZ) LC554; GA ČR(CZ) GD305/08/H037 Institutional research plan: CEZ:AV0Z50110509 Keywords : delta-opioid receptor (DOR) * monovalent ions * G(i)1alfa protein Subject RIV: CE - Biochemistry Impact factor: 1.555, year: 2011

Impact of weight-based ribavirin with peginterferon alfa-2b in African Americans with hepatitis C virus genotype 1.

Science.gov (United States)

Jacobson, Ira M; Brown, Robert S; McCone, Jonathan; Black, Martin; Albert, Clive; Dragutsky, Michael S; Siddiqui, Firdous A; Hargrave, Thomas; Kwo, Paul Y; Lambiase, Louis; Galler, Greg W; Araya, Victor; Freilich, Bradley; Harvey, Joann; Griffel, Louis H; Brass, Clifford A

2007-10-01

WIN-R (Weight-based dosing of pegINterferon alfa-2b and Ribavirin) was a multicenter, randomized, open-label, investigator-initiated trial involving 236 community and academic sites in the United States, comparing response to pegylated interferon (PEG-IFN) alfa-2b plus a flat or weight-based dose of ribavirin (RBV) in treatment-naive patients with chronic hepatitis C and compensated liver disease. Patients were randomized to receive PEG-IFN alfa-2b at 1.5 microg/kg/week plus flat-dose (800 mg/day) or weight-based-dose RBV (800 mg/day for weight 85-105 kg, or 1400 mg/day for >105- or =65 kg was the primary end point. Low SVR rates have been reported among African American individuals, in whom there is a preponderance of HCV genotype 1. This subanalysis of WIN-R was conducted to evaluate the efficacy of weight-based dosing among African American individuals with genotype 1 infection enrolled in the trial. Of 362 African American patients in the primary efficacy analysis, 188 received RBV flat dosing and 174 received weight-based dosing. SVR rates were higher (21% versus 10%; P = 0.0006) and relapse rates were lower (22% versus 30%) in the weight-based-dose group than in the flat-dose group. Safety and rates of drug discontinuation were similar between the 2 groups. Weight-based dosing of RBV is more effective than flat dosing in combination with PEG-IFN alfa-2b in African American individuals with HCV genotype 1. Even with weight-based dosing, response rates in African American individuals are lower than reported in other ethnic groups.

Fluorouracil continuous infusion plus alfa interferon plus oral folinic acid in advanced colorectal cancer

NARCIS (Netherlands)

Punt, C. J.; de Mulder, P. H.; Burghouts, J. T.; Wagener, D. J.

1992-01-01

Several reports on fluorouracil (5-FU) and alfa interferon (IFN-alpha) combination therapy in patients with advanced colorectal cancer have been published. In our study high-dose continuous infusion 5-FU (60 mg/kg per 48 hours), oral folinic acid (FA) (8 x 90 mg during 5-FU), and IFN-alpha three

Stroke in patients with type 2 diabetes mellitus, chronic kidney disease, and anemia treated with Darbepoetin Alfa

DEFF Research Database (Denmark)

Skali, Hicham; Parving, Hans-Henrik; Parfrey, Patrick S

2011-01-01

More strokes were observed in the Trial to Reduce Cardiovascular Events With Aranesp Therapy (TREAT) among patients assigned to darbepoetin alfa. We sought to identify baseline characteristics and postrandomization factors that might explain this association....

Fast radio burst discovered in the Arecibo pulsar ALFA survey

International Nuclear Information System (INIS)

Spitler, L. G.; Freire, P. C. C.; Lazarus, P.; Lee, K. J.; Cordes, J. M.; Chatterjee, S.; Wharton, R. S.; Brazier, A.; Hessels, J. W. T.; Lorimer, D. R.; McLaughlin, M. A.; Crawford, F.; Deneva, J. S.; Kaspi, V. M.; Karako-Argaman, C.; Allen, B.; Bogdanov, S.; Camilo, F.; Jenet, F. A.; Knispel, B.

2014-01-01

Recent work has exploited pulsar survey data to identify temporally isolated, millisecond-duration radio bursts with large dispersion measures (DMs). These bursts have been interpreted as arising from a population of extragalactic sources, in which case they would provide unprecedented opportunities for probing the intergalactic medium; they may also be linked to new source classes. Until now, however, all so-called fast radio bursts (FRBs) have been detected with the Parkes radio telescope and its 13-beam receiver, casting some concern about the astrophysical nature of these signals. Here we present FRB 121102, the first FRB discovery from a geographic location other than Parkes. FRB 121102 was found in the Galactic anti-center region in the 1.4 GHz Pulsar Arecibo L-band Feed Array (ALFA) survey with the Arecibo Observatory with a DM = 557.4 ± 2.0 pc cm –3 , pulse width of 3.0 ± 0.5 ms, and no evidence of interstellar scattering. The observed delay of the signal arrival time with frequency agrees precisely with the expectation of dispersion through an ionized medium. Despite its low Galactic latitude (b = –0.°2), the burst has three times the maximum Galactic DM expected along this particular line of sight, suggesting an extragalactic origin. A peculiar aspect of the signal is an inverted spectrum; we interpret this as a consequence of being detected in a sidelobe of the ALFA receiver. FRB 121102's brightness, duration, and the inferred event rate are all consistent with the properties of the previously detected Parkes bursts.

Fast radio burst discovered in the Arecibo pulsar ALFA survey

Energy Technology Data Exchange (ETDEWEB)

Spitler, L. G.; Freire, P. C. C.; Lazarus, P.; Lee, K. J. [Max-Planck-Institut für Radioastronomie, D-53121 Bonn (Germany); Cordes, J. M.; Chatterjee, S.; Wharton, R. S.; Brazier, A. [Department of Astronomy and Space Sciences, Cornell University, Ithaca, NY 14853 (United States); Hessels, J. W. T. [ASTRON, Netherlands Institute for Radio Astronomy, Postbus 2, 7990 AA, Dwingeloo (Netherlands); Lorimer, D. R.; McLaughlin, M. A. [Department of Physics and Astronomy, West Virginia University, Morgantown, WV 26506 (United States); Crawford, F. [Department of Physics and Astronomy, Franklin and Marshall College, Lancaster, PA 17604-3003 (United States); Deneva, J. S. [Naval Research Laboratory, 4555 Overlook Ave SW, Washington, DC 20375 (United States); Kaspi, V. M.; Karako-Argaman, C. [Department of Physics, McGill University, Montreal, QC H3A 2T8 (Canada); Allen, B. [Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI 53211 (United States); Bogdanov, S.; Camilo, F. [Columbia Astrophysics Laboratory, Columbia University, New York, NY 10027 (United States); Jenet, F. A. [Center for Gravitational Wave Astronomy, University of Texas at Brownsville, Brownsville, TX 78520 (United States); Knispel, B., E-mail: lspitler@mpifr-bonn.mpg.de [Leibniz Universität, Hannover, D-30167 Hannover (Germany); and others

2014-08-01

Recent work has exploited pulsar survey data to identify temporally isolated, millisecond-duration radio bursts with large dispersion measures (DMs). These bursts have been interpreted as arising from a population of extragalactic sources, in which case they would provide unprecedented opportunities for probing the intergalactic medium; they may also be linked to new source classes. Until now, however, all so-called fast radio bursts (FRBs) have been detected with the Parkes radio telescope and its 13-beam receiver, casting some concern about the astrophysical nature of these signals. Here we present FRB 121102, the first FRB discovery from a geographic location other than Parkes. FRB 121102 was found in the Galactic anti-center region in the 1.4 GHz Pulsar Arecibo L-band Feed Array (ALFA) survey with the Arecibo Observatory with a DM = 557.4 ± 2.0 pc cm{sup –3}, pulse width of 3.0 ± 0.5 ms, and no evidence of interstellar scattering. The observed delay of the signal arrival time with frequency agrees precisely with the expectation of dispersion through an ionized medium. Despite its low Galactic latitude (b = –0.°2), the burst has three times the maximum Galactic DM expected along this particular line of sight, suggesting an extragalactic origin. A peculiar aspect of the signal is an inverted spectrum; we interpret this as a consequence of being detected in a sidelobe of the ALFA receiver. FRB 121102's brightness, duration, and the inferred event rate are all consistent with the properties of the previously detected Parkes bursts.

FTIR spectroscopy and X-ray powder diffraction characterization of microcrystalline cellulose obtained from alfa fibers

Directory of Open Access Journals (Sweden)

Trache D.

2013-07-01

Full Text Available Many cereal straws have been used as raw materials for the preparation of microcrystalline cellulose (MCC. These raw materials were gradually replaced with wood products; nevertheless about 10% of the world overall pulp production is obtained from non-wood raw material. The main interest in pulp made from straw is that it provides excellent fibres for different industries with special properties, and that it is the major available source of fibrous raw material in some geographical areas. The aim of the present work was to characterize microcrystalline cellulose prepared from alfa fibers using the hydrolysis process. The products obtained are characterized with FTIR spectroscopy and X-ray powder diffraction. As a result, FTIR spectroscopy is an appropriate technique for studying changes occurred by any chemical treatment. The spectrum of alfa grass stems shows the presence of lignin and hemicelluloses. However, the cellulose spectrum indicates that the extraction of lignin and hemicellulose was effective. The X-ray analysis indicates that the microcrystalline cellulose is more crystalline than the source material.

A randomised, double-blind, placebo-controlled, crossover study to assess the efficacy and safety of three dosing schedules of agalsidase alfa enzyme replacement therapy for Fabry disease.

Science.gov (United States)

Hughes, D A; Deegan, P B; Milligan, A; Wright, N; Butler, L H; Jacobs, A; Mehta, A B

2013-07-01

Anecdotal reports suggest that the currently approved dosing interval of agalsidase alfa (0.2 mg/kg/2 weeks) for Fabry disease treatment is too long. This randomised, double-blind, placebo-controlled, crossover study investigated three altered dosing intervals. 18 Fabry patients received three agalsidase alfa dosing schedules, each for four weeks (A: 0.2 mg/kg∗2 weeks, B: 0.1 mg/kg/week, C: 0.2 mg/kg/week). Health state, pain levels, sweat volume and latency and plasma and urinary globotriaosylceramide levels were recorded throughout the study. No significant differences were found among the schedules for the primary efficacy outcome of self-assessed health state, or for pain scores. A trend toward increased sweat volume on QSART testing, and reduced urine globotriaosylceramide concentration were seen with treatment schedule C. Agalsidase alfa was safe and well tolerated with all schedules. In conclusion, the primary analyses did not find weekly infusions of agalsidase alfa to be statistically better than the approved dosing schedule however the data indicates that further studies with more patients over a longer period are required to more accurately determine the optimum dose and schedule. Copyright © 2013 Elsevier Inc. All rights reserved.

Antiviral activity of telaprevir (VX-950) and peginterferon alfa-2a in patients with hepatitis C

NARCIS (Netherlands)

Forestier, Nicole; Reesink, Hendrik W.; Weegink, Christine J.; McNair, Lindsay; Kieffer, Tara L.; Chu, Hui-May; Purdy, Susan; Jansen, Peter L. M.; Zeuzem, Stefan

2007-01-01

Telaprevir (VX-950), an inhibitor of the hepatitis C virus (HCV) NS3/4A protease, substantially decreased plasma HCV RNA levels in a prior clinical study. The present study evaluated viral kinetics and safety during dosing with telaprevir alone and in combination with peginterferon alfa-2a for 14

Using ALFA for high throughput, distributed data transmission in the ALICE O2 system

Science.gov (United States)

Wegrzynek, A.; ALICE Collaboration

2017-10-01

ALICE (A Large Ion Collider Experiment) is a heavy-ion detector designed to study the physics of strongly interacting matter (the Quark-Gluon Plasma at the CERN LHC (Large Hadron Collider). ALICE has been successfully collecting physics data in Run 2 since spring 2015. In parallel, preparations for a major upgrade of the computing system, called O2 (Online-Offline), scheduled for the Long Shutdown 2 in 2019-2020, are being made. One of the major requirements of the system is the capacity to transport data between so-called FLPs (First Level Processors), equipped with readout cards, and the EPNs (Event Processing Node), performing data aggregation, frame building and partial reconstruction. It is foreseen to have 268 FLPs dispatching data to 1500 EPNs with an average output of 20 Gb/s each. In overall, the O2 processing system will operate at terabits per second of throughput while handling millions of concurrent connections. The ALFA framework will standardize and handle software related tasks such as readout, data transport, frame building, calibration, online reconstruction and more in the upgraded computing system. ALFA supports two data transport libraries: ZeroMQ and nanomsg. This paper discusses the efficiency of ALFA in terms of high throughput data transport. The tests were performed with multiple FLPs pushing data to multiple EPNs. The transfer was done using push-pull communication patterns and two socket configurations: bind, connect. The set of benchmarks was prepared to get the most performant results on each hardware setup. The paper presents the measurement process and final results - data throughput combined with computing resources usage as a function of block size. The high number of nodes and connections in the final set up may cause race conditions that can lead to uneven load balancing and poor scalability. The performed tests allow us to validate whether the traffic is distributed evenly over all receivers. It also measures the behaviour of

The influence of a polymorphism in the gene encoding angiotensin converting enzyme (ACE on treatment outcomes in late-onset Pompe patients receiving alglucosidase alfa

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Rena C. Baek

2016-09-01

Full Text Available Correlations between angiotensin-converting enzyme (ACE genotype (I/I, I/D, D/D, disease severity at baseline and response to enzyme replacement therapy (ERT were assessed in the Pompe disease Late-Onset Treatment Study (LOTS. No correlations were observed between ACE genotype and disease severity at baseline. However, D/D patients appeared to have a reduced response to alglucosidase alfa treatment than I/I or I/D patients, suggesting that ACE polymorphisms may influence the response to alglucosidase alfa treatment and warrants further investigation.

Corifollitropin alfa compared to daily rFSH or HP-HMG in GnRH antagonist controlled ovarian stimulation protocol for patients undergoing assisted reproduction.

Science.gov (United States)

Souza, Priscila Morais Galvão; Carvalho, Bruno Ramalho de; Nakagawa, Hitomi Miura; Rassi, Thalita Reis Esselin; Barbosa, Antônio César Paes; Silva, Adelino Amaral

2017-06-01

This study aimed to compare the outcomes of controlled ovarian stimulation (COS) with corifollitropin alfa versus daily recombinant follicle-stimulating hormone (rRFSH) or highly purified human menopausal gonadotropin (HP-HMG) in patients undergoing in vitro fertilization (IVF) cycles based on gonadotropin-releasing hormone (GnRH) antagonist protocols. The primary endpoints were total number of oocytes and mature oocytes. This retrospective study looked into 132 controlled ovarian stimulation cycles from IVF or oocyte cryopreservation performed in a private human reproduction center between January 1 and December 31, 2014. Enrollment criteria: women aged 0.05). There were no significant differences in fertilization (76.9% vs. 76.8%, p=1.0), biochemical pregnancy (66.7% vs. 47.2%, p=0.1561) or embryo implantation rates (68.7% vs. 50%, p=0.2588) between the groups using corifollitropin alfa and rFSH or HMG, respectively. Corifollitropin alfa seems to be as effective as rFSH or HP-HMG when used in the first seven days of ovulation induction for patients undergoing assisted reproduction in GnRH antagonist protocols.

Fungal Alfa Amilaz ve Deaktif Kuru Maya Preparatları Kullanılarak Hızlandırılmış Ekmek Yapımı

OpenAIRE

Bilgiçli, Nermin; Türker, Selman; Elgün, Adem

2015-01-01

The aim of this research is the determination of optimum fermentation time giving the best bread quality with fungal alfa amylase and a reducing agent-deactive dry yeast preparetes at different flour types in bread making. In the experiment two different enzyme praperates (Fungal Alfa Amylase and Reducing Agent-Deactive dry yeast) were added at three different types of flour (Type 550, 650 and 850) and prepared bread dough. The dough was rested during three different bulk fermentation time (1...

Teores de retinol, beta-caroteno e alfa-tocoferol em leites bovinos comercializados na cidade de São Paulo Amounts of retinol, beta-carotene and alpha-tocopherol in cow milk comercialized in the city of São Paulo

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Rute BIANCHINI

1999-12-01

Full Text Available Os teores de retinol, beta-caroteno e alfa-tocoferol foram determinados por cromatografia líquida de alta eficiência em leites em pó, pasteurizados e esterilizados, comercializados na Cidade de São Paulo. Após a saponificação e extração, os compostos foram determinados simultaneamente utilizando-se coluna de sílica, fase móvel constituída por hexano:isopropanol (99:1 e fluxo de 2,0mL/min. O retinol e o beta-caroteno foram determinados no detector UV/visível e o alfa-tocoferol no detector de fluorescência, ligado em série com o anterior. Os valores de vitamina A dos leites foram calculados com e sem a consideração do beta-caroteno. A maior contribuição deste nutriente no valor de vitamina A esteve entre os leites em pó, cerca de 17% em uma das marcas. Os altos teores das vitamina A e E encontrados em alguns leites, indicam que os mesmos provavelmente receberam adição destas vitaminas, não trazendo, entretanto, tal informação no rótulo. A análise de vitaminas nestes produtos indica a necessidade de maior controle de qualidade dos mesmos.The amount of retinol, beta-carotene, alpha -tocopherol in powder, pasteurized and sterilized milk, comercialized in the city of São Paulo, were analyzed by high performance liquid chromatography. After saponification and extraction, compounds were determined simultaneously through a normal-phase column, mobile phase composed by hexan:2-propanol (99:1 and 2 mL/min flow. The retinol and beta-carotene were analysed by a UV/visible detector and the alpha-tocopherol by a fluorescence detector, both linked in series. The milk vitamin A values were calculated with and without beta-carotene. The major contribution of beta-carotene in the vitamin A value was in powder milks, around 17% in one of the brands. The high amounts of vitamin A and E found in some milks indicate that they probably were enriched with these vitamins but nothing is mentioned about this in their labels. The analysis of

Estudo da Reação da Desitratação da Gipsita Em Meio Aquoso Num Reator de Batelada (parr) Para Produção de Gesso Alfa

OpenAIRE

Pereira, Pedro Leiz de Mendonça

2012-01-01

A partir da reação de desidratação da gipsita (CaSO4.2H2O) pode-se obter o Gesso (CaSO4.1/2H2O) do tipo alfa e do tipo beta, dependendo das condições nas quais ocorre a reação. A produção do gesso alfa é mais complexa, por tratar-se de um produto com características de maior valor agregado e com aplicação diferenciada. O presente trabalho visa o estudo dos aspectos termocinéticos da reação de desidratação da gipsita para obtenção do gesso alfa em meio aquoso, e procura trazer maiores informaç...

Daclatasvir plus peginterferon alfa and ribavirin for treatment-naive chronic hepatitis C genotype 1 or 4 infection

DEFF Research Database (Denmark)

Hézode, Christophe; Hirschfield, Gideon M; Ghesquiere, Wayne

2015-01-01

OBJECTIVE: To evaluate the safety and efficacy of daclatasvir, an HCV NS5A inhibitor with pangenotypic activity, administered with peginterferon-alfa-2a/ribavirin. DESIGN: In this Phase 2b double-blind, placebo-controlled study, treatment-naive adults with HCV genotype 1 (N=365) or 4 (N=30...

Isolamento e caracterização de um mutante de saccharomyces cerevisiae com características fenotípicas opostas à cepa pkc

OpenAIRE

Gomes, Katia das Neves

2004-01-01

Em leveduras, a proteína quinase C participa da regulação da via bioquímica responsável pela transcrição de uma subunidade da enzima glucano sintase, a qual está envolvida na síntese da parede celular. A via PKC MAP quinase consiste das enzimas Bck1, Mkk1/2 e Mpk1 que são ativadas por fosforilação. Recentemente, nós descobrimos que o mutante pkc1 D, contrariamente aos demais mutantes da cascata Map quinase, exibe dois principais defeitos no controle do metabolismo de carbono. A cepa pkc1 D ap...

SPECIFIC DISORDERS OF THE RESPIRATORY SYSTEM IN CYSTIC FIBROSIS. CLINICAL EFFICACY OF THERAPY WITH DORNASE ALFA IN CHILDREN

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T.V. Simanova

2010-01-01

Full Text Available The article is devoted to specific disorders of the respiratory system in cystic fibrosis. 64 patients with cystic fibrosis (CF aged 2 months to 32 years and residing in the Udmurtian Republic were studied. Epidemiological and genetic specifics of this disease in the mentioned region of the RF were examined. Clinical, X-ray, functional and microbiological studies of the CF patients’ respiratory system were conducted. It was found that genotype delF508 and chronic infection Pseudomonas aeruginosa, Staphylococcus aureus cause severe structural changes to the bronchopulmonary system more often. The obtained data suggest the advisability of identifying the groups of CF patients at the highest risk of severe respiratory system disorders in order to optimise therapeutic efforts. The article provides indicators of clinical efficacy of a dornase alfa therapy in CF children.Key words: cystic fibrosis, genotype, delF508 mutation, respiratory organs, pseudomonas aeruginosa infection, staphylococcal infection, respiratory function, mucolytic function, dornase alfa. (Pediatric Pharmacology. – 2010; 7(6:44-48

Forward Detectors in ATLAS: ALFA, ZDC and LUCID

CERN Document Server

Fabbri, L; The ATLAS collaboration

2009-01-01

In order to determine the experimental cross sections for the observed physics processes, an estimation of the absolute luminosity is needed. In fact a careful study of “well known” processes will be one of the first steps of the LHC experiments as it can provide possible signatures of new physics which consist in deviations with respect to the Standard Model (SM) predictions. The methodologies for luminosity monitoring and total cross section estimation at the LHC will be reviewed in this talk along with the dedicated detectors of the ATLAS experiment. ATLAS will make extensive usage of the detectors in the forward region each one with a different task: LUCID (LUminosity measurement using Cherenkov Integrating Detector) is a system of 40 (2 x 20) Cherenkov tubes, surrounding the beam pipe at about 17 m from the interaction region. It will be able to monitor the collision-by-collision luminosity by detecting and counting the number of charged particles coming from the impact point. ALFA (Absolute Luminosi...

Efeito da idade e da suplementação oral com o acetato de DL-alfa-tocoferol sobre os níveis séricos de vitamina E e sobre o metabolismo oxidativo dos neutrófilos em bezerros

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J. N. Costa

2006-03-01

Full Text Available Este trabalho teve por objetivo avaliar o efeito da idade e da suplementação oral com acetato de DL-alfa-tocoferol sobre os níveis séricos de vitamina E e sobre o metabolismo oxidativo dos neutrófilos de bezerros da raça Jersey do nascimento aos 45 dias de idade. Foram utilizados 14 bezerros divididos em dois grupos de sete animais cada. O grupo tratamento recebeu 2g de acetato de DL-alfa-tocoferol, por via oral, diariamente, até os 45 dias de vida e o controle não recebeu nenhum tipo de suplementação para esta vitamina. Foram colhidas amostras de soro sanguíneo ao nascimento, aos cinco, 10, 20, 30 e 45 dias de idade, para: determinação sérica da vitamina E e para a avaliação da função dos neutrófilos. Concluímos que a suplementação oral com acetato de DL-alfa-tocoferol, na dose de 2 g/dia/VO, do nascimento aos 45 dias de idade aumentou significativamente (p≤ 0,05 os níveis séricos de vitamina E em bezerros. Os menores percentuais de neutrófilos redutores foram detectados logo após o nascimento e os maiores aos 45 dias de idade. A suplementação com vitamina E não alterou significativamente a função oxidativa dos neutrófilos, mas os valores superiores obtidos para os animais do grupo tratado, para o teste estimulado, sugerem uma ação neutrófilica mais potente diante uma infecção natural nesses animais.

Experimental investigation of the mechanical properties of Alfas stone

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Konstas N. Kaklis

2017-04-01

Full Text Available This paper focuses on the experimental investigation of the mechanical properties of the Alfas natural building stone. Two series of uniaxial compression tests and indirect tensile tests (Brazilian tests were performed in order to determine the uniaxial compressive strength and the indirect tensile strength respectively. Different sets of cylindrical specimens and circular discs were prepared by varying their geometry in order to examine the size effect on the respective strength values. Also, the size effect was investigated with respect to the calculated intact rock modulus and Poisson’s ratio. All specimens were prepared by following the ISRM suggested methods and the load was applied using a stiff 1600 kN MTS hydraulic testing machine and a 500 kN load cell. Strain was measured using biaxial 0/90 stacked rosettes appropriately attached on each specimen.

The Arecibo Legacy Fast ALFA Survey : X. The HI Mass Function and Omega_HI From the 40% ALFALFA Survey

NARCIS (Netherlands)

Martin, Ann M.; Papastergis, Emmanouil; Giovanelli, Riccardo; Haynes, Martha P.; Springob, Christopher M.; Stierwalt, Sabrina

2010-01-01

The Arecibo Legacy Fast ALFA (ALFALFA) survey has completed source extraction for 40% of its total sky area, resulting in the largest sample of HI-selected galaxies to date. We measure the HI mass function from a sample of 10,119 galaxies with 6.2

DAHANCA 10 – Effect of darbepoetin alfa and radiotherapy in the treatment of squamous cell carcinoma of the head and neck. A multicenter, open-label, randomized, phase 3 trial by the Danish head and neck cancer group

DEFF Research Database (Denmark)

Overgaard, Jens; Hoff, Camilla Molich; Hansen, Hanne Sand

2018-01-01

Purpose: To evaluate if correction of low hemoglobin (Hb) levels by means of darbepoetin alfa improves the outcomes of radiotherapy in patients with squamous cell carcinoma of the head and neck (HNSCC). Patients and methods: Patients eligible for primary radiotherapy and who had Hb values below 14.......0 g/dl were randomized to receive accelerated fractionated radiotherapy with or without darbepoetin alfa. Patients also received the hypoxic radiosensitizer nimorazole. Darbepoetin alfa was given weekly during radiotherapy or until the Hb value exceeded 15.5 g/dl. Results: Following a planned interim...... distributed according to the stratification parameters (gender, T and N staging, tumor site). Treatment with darbepoetin alfa increased the Hb level to the planned value in 81% of the patients. The compliance was good without excess serious adverse events. The results showed a poorer outcome with a 5-year...

Ca(OH2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS = Ação do Ca(OH2 sobre a produção de TNF-alfa e NO de cultura de macrófagos estimulada por LPS de Pseudomonas aeruginosa

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Queiroz, Celso Emanoel de Souza

2008-01-01

Full Text Available Objetivo: A ação do hidróxido de cálcio [Ca(OH2] com o sistema imune e o mecanismo de neutralização das bactérias e seus subprodutos ainda não foi completamente esclarecida. Neste estudo foi avaliada a capacidade do Ca(OH2 em neutralizar o lipopolissacarídeo (LPS de Pseudomonas aeruginosa, utilizando-se duas metodologias: liberação de Óxido Nítrico (NO e Fator de Necrose Tumoral Alfa (TNF-alfa em cultura de macrófagos peritoneais de camundongos. Metodologia: No ensaio do NO, as células peritoneais foram expostas a uma solução de LPS (25mg/mL e 50mg/mL; e à suspensão de LPS/Ca(OH2 em duas concentrações (50mg/ 25mg e 25mg/25mg. Após 8 horas de incubação, foi utilizado reagente de Griess, e a liberação de NO foi quantificada. No ensaio do TNF-alfa, a solução de LPS foi usada na concentração de 25mg/mL e o LPS/Ca(OH2 a 25mg/25mg. Após 24 horas, as células foram fixadas e coradas com cristal violeta, e os valores de absorbância foram obtidos. Os resultados foram expressos em micromols. Todos os testes foram realizados em triplicata. Resultados: A presença de Ca(OH2 nas duas concentrações avaliadas reduziu significativamente a liberação de NO e TNF-alfa. Conclusão: Pode-se concluir que o LPS bacteriano representa um forte estímulo para liberação destas citocinas, mas o hidróxido de cálcio foi capaz de neutralizar este efeito

Deficiência de alfa-1 antitripsina: diagnóstico e tratamento Alpha-1 antitrypsin deficiency: diagnosis and treatment

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Aquiles A Camelier

2008-07-01

Full Text Available A deficiência de alfa-1 antitripsina é um distúrbio genético de descoberta recente e que ocorre com freqüência comparável à da fibrose cística. Resulta de diferentes mutações no gene SERPINA1 e tem diversas implicações clínicas. A alfa-1 antitripsina é produzida principalmente no fígado e atua como uma antiprotease. Tem como principal função inativar a elastase neutrofílica, impedindo a ocorrência de dano tecidual. A mutação mais freqüentemente relacionada à doença clínica é o alelo Z, que determina polimerização e acúmulo dentro dos hepatócitos. O acúmulo e a conseqüente redução dos níveis séricos de alfa-1 antitripsina determinam, respectivamente, doença hepática e pulmonar, sendo que esta se manifesta principalmente sob a forma de enfisema de aparecimento precoce, habitualmente com predomínio basal. O diagnóstico envolve a detecção de níveis séricos reduzidos de alfa-1 antitripsina e a confirmação fenotípica. Além do tratamento usual para doença pulmonar obstrutiva crônica, existe atualmente uma terapia específica com infusão de concentrados de alfa-1 antitripsina. Essa terapia de reposição, aparentemente segura, ainda não teve a eficácia clínica definitivamente comprovada, e o custo-efetividade também é um tema controverso e ainda pouco abordado. Apesar da sua importância, não existem dados epidemiológicos brasileiros a respeito da prevalência da doença ou da freqüência de ocorrência dos alelos deficientes. O subdiagnóstico também tem sido uma importante limitação tanto para o estudo da doença quanto para o tratamento adequado dos pacientes. Espera-se que a criação do Registro Internacional de Alfa-1 venha a resolver essas e outras importantes questões.Alpha-1 antitrypsin deficiency is a recently identified genetic disease that occurs almost as frequently as cystic fibrosis. It is caused by various mutations in the SERPINA1 gene, and has numerous clinical

Uso tópico do inteferon alfa-2b para neoplasia escamosa da superficie ocular em um caso de paciente com xeroderma pigmentoso

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Nayara Queiroz Cardoso Pinto

Full Text Available Resumo Relato de um caso clínico de Xeroderma Pigmentoso com carcinoma espinocelular de conjuntiva bilateral que apresentou regressão importante das dimensões tumorais com o uso de Interferon alfa-2b tópico. Relato de caso: Paciente feminina com Xeroderma Pigmentoso em estágio avançado, com ausência de pele sadia, tendo sido submetida a cerca de 60 exéreses de lesões de pele malignas. A paciente compareceu com tumoração conjuntival em ambos os olhos, correspondendo a carcinoma espinocelular de conjuntiva e neoplasia intraepitelial de conjuntiva em olho esquerdo. Devido as dificuldades cirúrgicas, alta taxa de recidiva e elevada probabilidade de formação de simbléfaro foi-se iniciado terapêutica com Interferon alfa-2beta 1.000.000 unidades tópico, obtendo-se bons resultados com importante regressão do tamanho da lesão e resolução dos sintomas. Conclusão: O uso tópico de interferon alfa-2beta em neoplasia escamosa de conjuntiva, mostrou-se uma boa opção terapêutica em situações de elevado risco cirúrgico e de complicações pós operatórias.

An Open-label, Single-dose, Pharmacokinetic Study of Factor VIII Activity After Administration of Moroctocog Alfa (AF-CC) in Male Chinese Patients With Hemophilia A.

Science.gov (United States)

Liu, Hongzhong; Wu, Runhui; Hu, Pei; Sun, Feifei; Xu, Lihong; Liang, Yali; Nepal, Sunil; Qu, Peng Roger; Huard, Francois; Korth-Bradley, Joan M

2017-07-01

Hemophilia A represents up to 80% of all hemophilia cases in China. In patients with this condition, bleeding can be prevented and controlled by administering clotting factor VIII (FVIII). Since their initial availability, recombinant FVIII products have undergone several iterations to enhance their safety. Moroctocog alfa albumin-free cell culture (AF-CC) is among the third generation of recombinant FVIII products and received regulatory approval in China in August 2012. The present study characterizes the single-dose pharmacokinetic parameters of FVIII activity (FVIII:C) after administration of moroctocog alfa (AF-CC) in male Chinese patients with hemophilia A. This multicenter, open-label, single-dose study enrolled 13 male Chinese patients diagnosed with severe hemophilia A (FVIII:C hemophilia A. The pharmacokinetic profile in older patients was similar to that previously reported with recombinant FVIII products in studies with a predominantly white population; younger patients had reduced exposure to FVIII:C. The single doses of moroctocog alfa (AF-CC) were well tolerated; 2 cases of transient, low-titer FVIII inhibitor development were observed. ClinicalTrials.gov identifier: NCT02461992. Copyright © 2017 Elsevier HS Journals, Inc. All rights reserved.

Peginterferon alfa-2a and ribavirin in Latino and non-Latino whites with hepatitis C.

Science.gov (United States)

Rodriguez-Torres, Maribel; Jeffers, Lennox J; Sheikh, Muhammad Y; Rossaro, Lorenzo; Ankoma-Sey, Victor; Hamzeh, Fayez M; Martin, Paul

2009-01-15

Race has been shown to be a factor in the response to therapy for hepatitis C virus (HCV) infection, and limited data suggest that ethnic group may be as well; however, Latinos and other ethnic subpopulations have been underrepresented in clinical trials. We evaluated the effect of Latino ethnic background on the response to treatment with peginterferon alfa-2a and ribavirin in patients infected with HCV genotype 1 who had not been treated previously. In a multicenter, open-label, nonrandomized, prospective study, 269 Latino and 300 non-Latino whites with HCV infection received peginterferon alfa-2a, at a dose of 180 microg per week, and ribavirin, at a dose of 1000 or 1200 mg per day, for 48 weeks, and were followed through 72 weeks. The primary end point was a sustained virologic response. We enrolled Latinos whose parents and grandparents spoke Spanish as their primary language; nonwhite Latinos were excluded. Baseline characteristics were similar in the Latino and non-Latino groups, although higher proportions of Latino patients were 40 years of age or younger, had a body-mass index (BMI, the weight in kilograms divided by the square of the height in meters) of more than 27 or more than 30, and had cirrhosis. The rate of sustained virologic response was higher among non-Latino whites than among Latinos (49% vs. 34%, PLatino whites at week 4 (P=0.045) and throughout the treatment period (PLatino or non-Latino background was an independent predictor of the rate of sustained virologic response in an analysis adjusted for baseline differences in BMI, cirrhosis, and other characteristics. Adherence to treatment did not differ significantly between the two groups. The numbers of patients with adverse events and dose modifications were similar in the two groups, but fewer Latino patients discontinued therapy because of adverse events. Treatment with peginterferon alfa-2a and ribavirin for 48 weeks resulted in rates of sustained virologic response among patients

Interferon alfa for chronic hepatitis B infection: increased efficacy of prolonged treatment. The European Concerted Action on Viral Hepatitis (EUROHEP)

NARCIS (Netherlands)

Janssen, H. L.; Gerken, G.; Carreño, V.; Marcellin, P.; Naoumov, N. V.; Craxi, A.; Ring-Larsen, H.; Kitis, G.; van Hattum, J.; de Vries, R. A.; Michielsen, P. P.; ten Kate, F. J.; Hop, W. C.; Heijtink, R. A.; Honkoop, P.; Schalm, S. W.

1999-01-01

Interferon alfa (IFN-alpha) is the primary treatment for chronic hepatitis B. The standard duration of IFN-alpha therapy is considered 16 weeks; however, the optimal treatment length is still poorly defined. We evaluated the efficacy and acceptability of prolonged IFN-alpha treatment in patients

The frequency of β-globin gene haplotypes, α-thalassemia and genetic polymorphisms of methylenetetrahydrofolate reductase, factor V Leiden and prothrombin genes in children with sickle cell disease in Rio de Janeiro, Brazil Frequência dos haplótipos da globina beta, da talassemia alfa e dos polimorfismos genéticos dos genes da metilenotetrahidrofolato redutase, do fator V Leiden e da protrombina em crianças com doença falciforme no Rio de Janeiro, Brasil

Directory of Open Access Journals (Sweden)

Isaac L. Silva Filho

2010-02-01

Full Text Available A freqüência dos haplótipos beta S e beta C do gene da globina e a prevalência de talassemia alfa e de mutações nos genes da metilenotetrahidrofolato redutase (MTHFR-C677T, do fator V de Leiden e da protrombina (G20210A foi estudada em crianças com doença falciforme do Rio de Janeiro. O haplótipo Bantu foi o mais freqüente (65,9%, 21,2% das crianças (18% heterozigotas e 3% homozigotas apresentam talassemia com mutação alfa 3.7kb, ao contrário da mutação alfa 4.2kb que não foi encontrada. Os alelos 677CT e 677TT da MTHFR foram observados em 20,2% e 4,8%, respectivamente. Os haplótipos Camarões, Árabe-Indiano e Senegal não foram detectados na amostra estudada, bem como mutações no gene do fator V de Leiden e da protrombina. Somente o haplótipo beta C CI foi observado. Esse é o primeiro estudo realizado em uma amostra proveniente do Programa de Triagem Neonatal para Hemoglobinopatias do estado do Rio de Janeiro. Apesar do Rio de Janeiro ser a segunda maior cidade brasileira e seus habitantes expressarem o elevado grau de miscigenação ocorrida no país, nossos resultados ainda coincidem com os registros históricos dos fluxos migratórios do gene beta S para o Brasil, bem como refletem a forte influência de indivíduos de origem africana na população do Rio de Janeiro.

Nebulised dornase alfa versus placebo or hypertonic saline in adult critically ill patients

DEFF Research Database (Denmark)

Claudius, Casper; Perner, Anders; Møller, Morten Hylander

2015-01-01

a systematic review with meta-analysis and trial sequential analysis (TSA) using the Cochrane Collaboration methodology. Eligible trials were randomised clinical trials comparing nebulised dornase alfa with placebo, no prophylaxis, or hypertonic saline. The predefined outcome measures were all-cause mortality...... of the primary estimate was assessed by TSA. RESULTS: Two trials (n = 63) were included; both were judged to have high risk of bias. There was no statistically significant difference in mortality (random effects model RR (95 % CI) 0.73 (0.09-5.77); P = 0.24; I (2) = 30 %). TSA could not be conducted because less...

Bakterisel Alfa-Amilaz Önemi, Özellikleri ve Üretim Teknolojisi

OpenAIRE

Esener, A. Aydın

2015-01-01

Enzimler biyolojik katalizörlerdir. Çeşitli mikroorganizmalar, çevrelerini yaşamları için elverişli bir hale getirebilme çabası ile bazı enzimleri bünyelerinde sentez edip hücre duvarından dışarıya salgılamaktadırlar. Alfa-amilaz da bu tür bir enzim olup, özellikle nişasta gibi doğal kaynaklı ham maddelerin parçalanmasında kullanılmaktadır. Endüstride geniş tüketim olanakları bulan bu enzimin, modern yöntemlerle üretilmesi, özellikle fermente olabilir tarım artığı maddelerin bol olduğu Türkiy...

Caracterização de componentes da resposta TH17 em lesões ateroscleróticas humanas

OpenAIRE

Rômulo Tadeu Dias de Oliveira

2010-01-01

Resumo: A presença de células T ativadas em lesões ateroscleróticas confirma a sua participação na progressão da doença. Recentemente, um novo subtipo de linfócitos TCD4+ produtores de IL-17 (TH17) foi descrito e a sua associação a doenças de caráter inflamatório, bem como a sua capacidade de induzir a produção de mediadores frequentemente encontrados em lesões ateroscleróticas (IL-1?? TNF-? ??TGF-? ??CCL2 e MMP) sugerem que possam ter uma participação na aterosclerose. O objetivo do presente...

DeposiÃÃo de DiÃxido de Estanho-FlÃor (SnO2:F) em Substrato Transparente para Uso em CÃlulas FotoeletroquÃmicas

OpenAIRE

Francisco Marcone Lima

2013-01-01

Vidros condutores tÃm sido usados como Ãxidos condutores transparentes (OCTâs). Por causa da transmitÃncia na regiÃo visÃvel e baixa resistÃncia de filme os OCTâs tÃm sido usados como componente de cÃlulas fotoeletroquÃmicas ativadas por corante. Neste trabalho à feito vidros condutores, em escala de laboratÃrio, de dimensÃes 2,6 cm x 7,6 cm pela tÃcnica âspray pyrolysisâ, os vidros condutores sÃo aplicados na construÃÃo da cÃlula fotoeletroquÃmica com eletrodos imersos e da cÃlula de G...

Alfa hemolisina induce un aumento de calcio eritrocitario: estudio por FLIM (Fluorescence Lifetime Imaging Microscopy)

OpenAIRE

Sánchez, Susana; Bakás, Laura; Gratton, Enrico; Herlax, Vanesa Silvana

2010-01-01

Alfa hemolisina (HlyA), miembro representativo de la familia de toxinas RTX (Repeat in toxin), es una toxina proteica que ciertas cepas patógenas de E.coli secretan específicamente al medio. Está demostrado que la toxina es un factor importante de virulencia en enfermedades extraintestinales. Además, se ha determinado que el desarrollo de falla renal aguda aumenta significativamente la mortalidad en pacientes con shock séptico producido por bacterias Gram negativas, en los que distintos facto...

Evaluation of treatment response to enzyme replacement therapy with Velaglucerase alfa in patients with Gaucher disease using whole-body magnetic resonance imaging.

Science.gov (United States)

Laudemann, K; Moos, L; Mengel, E; Lollert, A; Hoffmann, C; Brixius-Huth, M; Wagner, D; Düber, C; Staatz, G

2016-03-01

This was a retrospective data analysis to evaluate the treatment response to enzyme replacement therapy (ERT) with Velaglucerase alfa using whole-body magnetic resonance imaging (MRI). A baseline and follow-up MRI were performed on 18 Gaucher Type 1 patients at an interval of 11.6 months. The MRI score systems determined the Bone-Marrow-Burden (BMB) score, the Düsseldorf-Gaucher score (DGS), and the Vertebra-Disc-Ratio (VDR). The Severity Score Index Type 1 (GD-DS3) was also assessed. The baseline MRI medians were: BMB, 7.00; DGS, 3.00; and VDR: 1.70; while, the follow-up MRI medians were: BMB, 7.00; DGS, 3.00; and VDR: 1.73. The baseline GD-DS3 median was 2.40 (BMB excl.: 0.50) and the follow-up median was 2.00 (BMB excl.: 0.50). There was weak statistical significance with the Wilcoxon signed-rank test for the DGS (p=0.034) and GD-DS3 (p=0.047) between both MRIs. Velaglucerase alfa therapy is a effective long-term treatment for Gaucher Type 1 patients who are newly diagnosed or switching therapies. Measurements with whole-body MRI and an objective scoring system were reliable tools for detecting early stage bone marrow activity. Further research is needed to evaluate the "Booster-Effect" of Velaglucerase alfa therapy in Gaucher skeletal disease. Copyright © 2015 Elsevier Inc. All rights reserved.

Evaluation of the efficacy and safety of three dosing regimens of agalsidase alfa enzyme replacement therapy in adults with Fabry disease

Directory of Open Access Journals (Sweden)

Goláň L

2015-07-01

Full Text Available Lubor Goláň,1 Ozlem Goker-Alpan,2 Myrl Holida,3 Ikka Kantola,4 Mariusz Klopotowski,5 Johanna Kuusisto,6 Aleš Linhart,1 Jacek Musial,7 Kathleen Nicholls,8 Derlis Gonzalez-Rodriguez,9 Reena Sharma,10 Bojan Vujkovac,11 Peter Chang,12 Anna Wijatyk12 1First Faculty of Medicine, Department of Cardiovascular Medicine, Charles University, Prague, Czech Republic; 2Lysosomal Research and Treatment Unit, Fairfax, VA, USA; 3Stead Family Department of Pediatrics, Division of Medical Genetics, University of Iowa Hospitals and Clinics, Iowa City, IA, USA; 4Division of Medicine, Turku University Hospital, Turku, Finland; 5Institute of Cardiology, Warsaw, Poland; 6Department of Medicine, Center for Medicine and Clinical Research, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland; 7Department of Internal Medicine, Jagiellonian University Medical College, Krakow, Poland; 8Department of Nephrology, Royal Melbourne Hospital and the University of Melbourne, VIC, Australia; 9Instituto Privado de Hematologia E Investigacion Clinica (IPHIC, Asuncion, Paraguay; 10Salford Royal NHS Foundation Trust, Salford, UK; 11General Hospital Slovenj Gradec, Slovenj Gradec, Slovenia; 12Shire, Lexington, MA, USA Purpose: Efficacy and safety of agalsidase alfa at 0.2 mg/kg weekly were compared with 0.2 mg/kg every other week (EOW. Exploratory analyses were performed for 0.4 mg/kg weekly.Patients and methods: This was a 53-week, Phase III/IV, multicenter, open-label study (NCT01124643 in treatment-naïve adults (≥18 years with Fabry disease. Inclusion criteria were left ventricular hypertrophy at baseline, defined as left ventricular mass indexed to height >50 g/m2.7 for males and >47 g/m2.7 for females. Primary endpoint was reduction of left ventricular mass indexed to height as assessed by echocardiography. Secondary endpoints included cardiac (peak oxygen consumption, 6-minute walk test, Minnesota Living with Heart Failure Questionnaire, New

Peginterferon alfa-2b and weight-based or flat-dose ribavirin in chronic hepatitis C patients: a randomized trial.

Science.gov (United States)

Jacobson, Ira M; Brown, Robert S; Freilich, Bradley; Afdhal, Nezam; Kwo, Paul Y; Santoro, John; Becker, Scott; Wakil, Adil E; Pound, David; Godofsky, Eliot; Strauss, Robert; Bernstein, David; Flamm, Steven; Pauly, Mary Pat; Mukhopadhyay, Pabak; Griffel, Louis H; Brass, Clifford A

2007-10-01

This prospective, multicenter, community-based and academic-based, open-label, investigator-initiated, U.S. study evaluated efficacy and safety of pegylated interferon (PEG-IFN) alfa-2b plus a flat or weight-based dose of ribavirin (RBV) in adults with chronic hepatitis C. Patients (n = 5027) were randomly assigned to receive PEG-IFN alfa-2b 1.5 microg/kg/week plus flat-dose (800 mg/day) or weight-based (800-1400 mg/day) RBV for 48 weeks (patients with genotype 1, 4, 5, or 6) and for 24 or 48 weeks (genotype 2/3 patients). Primary end point was sustained virologic response (undetectable [<125 IU/mL] serum hepatitis C virus RNA at 24-week follow-up). Sustained virologic response, but not end-of-treatment, rates were significantly higher with weight-based than with flat-dose RBV (44.2% versus 40.5%; P = 0.008). Sustained virologic response rates by intention-to-treat analysis were 34.0% and 28.9%, respectively, in genotype 1 patients (P = 0.005) and 31.2% and 26.7%, respectively, in genotype 1 patients with high baseline viral load (P = 0.056). In genotype 2/3 patients, rates were not significantly different (61.8% and 59.5%, respectively) regardless of treatment duration. Besides greater hemoglobin reductions with weight-based RBV, safety profiles were similar across RBV dosing groups, including the 1400-mg/day group. PEG-IFN alfa-2b plus weight-based RBV is more effective than flat-dose RBV, particularly in genotype 1 patients, providing equivalent efficacy across all weight groups. RBV 1400 mg/day is appropriate for patients 105 to 125 kg. For genotype 2/3 patients, 24 weeks of treatment with flat-dose RBV is adequate; no evidence of additional benefit of extending treatment to 48 weeks was demonstrated.

EFFECTIVENESS AND SAFETY OF VELAGLUCERASE ALFA IN TREATMENT OF GAUCHER DISEASE TYPE 1 (ACCORDING TO INTERNATIONAL STUDIES

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O. S. Gundobina

2014-01-01

Full Text Available The article is dedicated to modern approaches to treatment of Gaucher’s disease. The authors list the primary aspects of the disease and present data on the origin and introduction of pathogenetic enzyme replacement therapy to clinical practice. Review of international clinical studies demonstrates high effectiveness and safety of long-term enzyme replacement therapy with velaglucerase alfa in patients with confirmed type I Gaucher’s disease. 

Prospective exploratory muscle biopsy, imaging, and functional assessment in patients with late-onset Pompe disease treated with alglucosidase alfa: The EMBASSY Study

NARCIS (Netherlands)

A.T. van der Ploeg (Ans); Carlier, P.G. (Pierre G.); Carlier, R.-Y. (Robert-Yves); Kissel, J.T. (John T.); B. Schoser; Wenninger, S. (Stephan); Pestronk, A. (Alan); Barohn, R.J. (Richard J.); Dimachkie, M.M. (Mazen M.); Goker-Alpan, O. (Ozlem); Mozaffar, T. (Tahseen); Pena, L.D.M. (Loren D.M.); Simmons, Z. (Zachary); V. Straub; Guglieri, M. (Michela); Young, P. (Peter); Boentert, M. (Matthias); Baudin, P.-Y. (Pierre-Yves); S.C.A. Wens (Stephan); Shafi, R. (Raheel); Bjartmar, C. (Carl); B. Thurberg

2016-01-01

textabstractBackground Late-onset Pompe disease is characterized by progressive skeletal myopathy followed by respiratory muscle weakness, typically leading to loss of ambulation and respiratory failure. In this population, enzyme replacement therapy (ERT) with alglucosidase alfa has been shown to

Farelo de trigo e complexo enzimático na alimentação de poedeiras semipesadas na fase de produção Wheat bran and enzymatic complex in the feeding of laying hens in the production phase

Directory of Open Access Journals (Sweden)

Daniel de Magalhães Araujo

2008-05-01

Full Text Available O estudo foi conduzido com o objetivo de avaliar os efeitos da inclusão de farelo de trigo (FT na ração com ou sem a suplementação de um complexo enzimático (CE sobre o desempenho e a produção de ovos de frangas semipesadas na fase de produção. Foram utilizadas 288 frangas Lohmann Brown, distribuídas em delineamento inteiramente casualizado, em esquema fatorial 4 × 2, composto de quatro níveis de farelo de trigo (0, 3, 6 e 9% na ração e da suplementação de complexo enzimático (0 ou 100 g/100 kg de ração, com oito tratamentos e seis repetições de seis aves. O complexo enzimático continha as enzimas beta-galactosidase, galactomananase, xilanase e alfa-glucanase. O consumo de ração, o peso vivo final, a produção de ovos, o peso e a massa de ovos e a conversão por massa e por dúzia de ovos não foram afetados pela inclusão do farelo de trigo nas rações. A gravidade específica da casca dos ovos piorou com o aumento dos níveis de farelo de trigo nas dietas. Nenhuma característica foi afetada pela adição do complexo enzimático na ração, com exceção do peso do ovo, que melhorou de 62,74 para 64,28 g. Recomenda-se o uso de até 9% de farelo de trigo em rações para poedeiras em produção. A adição de alfa-galactosidase, galactomananase, xilanase e alfa-glucanase aumenta o peso dos ovos.The study was carried out with the objective to evaluate the effects of the inclusion of the wheat bran (WB with or without supplementation of an enzymatic complex (EC on the performance of semi-heavy hens in the egg-production phase. A total of 288 Lohmann Brown pullets were used, distributed to a completely randomized design in 4 × 2 factorial arrangement, composed by four WB levels (0, 3, 6 and 9% in the ration and enzymatic complex supplementation (0 or 100g/100 kg diet, with eight treatments and six replicates of six birds. The enzymatic complex contained the enzymes beta-galactosidase, galactomananase, xilanase and alpha

Effectiveness of interferon alfa on incidence of hepatocellular carcinoma and decompensation in cirrhosis type C. European Concerted Action on Viral Hepatitis (EUROHEP)

DEFF Research Database (Denmark)

Fattovich, G; Giustina, G; Degos, F

1997-01-01

The role of interferon alfa treatment in improving morbidity endpoints in patients with chronic hepatitis C infection is currently under debate. The aim of this study was to evaluate the effectiveness of interferon in preventing hepatocellular carcinoma and decompensation in cirrhosis type C....

Measurement of elastic proton-proton scattering at $\\sqrt{s} = 7$ TeV with the ALFA sub-detector of ATLAS at the LHC

CERN Document Server

Kreutzfeldt, Kristof; Stenzel, Hasko

The ATLAS experiment with the ALFA sub-detector, provides a unique opportunity to measure elastic proton--proton scattering at the LHC at a centre-of-mass energy of $\\sqrt{s} = 7$ TeV, that has never been reached before. The ALFA detector is a tracking detector housed in Roman Pots, which makes it possible to measure elastically scattered protons down to very small scattering angles. From the proton tracks, measured during a LHC fill with special $\\beta^{*} = 90$ m beam optics, the differential elastic cross-section as a function of the four-momentum transfer squared $t$ is determined, and the total hadronic cross-section $\\sigma_\\text{tot}$, the nuclear slope parameter $B$ and further derived quantities are extracted by utilizing the optical theorem. The total hadronic cross-section is a fundamental parameter of strong interaction depending on the centre-of-mass energy. It has been measured for more than 50 years at different energies and accelerators, where a rise with energy was observed. A newly developed...

Wykorzystanie metod analitycznych do wyznaczania wielowymiarowych alfa-stabilnych rozkładów prawdopodobieństwa

OpenAIRE

Łażewski, Marek; Zator, Krzysztof

2004-01-01

In this paper we study the relationship between multivariate a-stable probability distributions and their spectral measure. Analytical method based on nonabelian harmonic analysis is used to express the spectral measure using spherical harmonics on the sphere. Alfa-stabilne rozkłady prawdopodobieństwa, będące uogólnieniem rozkładów normalnych, mają ważną cechę, nie posiadają skończonej wariancji, która utrudnia stosowanie klasycznego formalizmu matematyczno-statystycznego do wyznac...

Effect of darbepoetin alfa on physical function in patients undergoing surgery for colorectal cancer

DEFF Research Database (Denmark)

Nørager, C B; Jensen, M B; Madsen, M R

2007-01-01

OBJECTIVE: To study whether perioperative treatment with darbepoetin alfa (DA) improves physical performance following colorectal cancer surgery. METHODS: Patients admitted for planned colorectal cancer surgery were randomized to receive either weekly placebo or DA 300 or 150 microg depending...... on the hemoglobin (Hb) concentration. Patients were assessed 10 days before, as well as 7 and 30 days after surgery for work capacity, postural sway, muscle strength, fatigue and quality of life (QoL). The primary outcome measure were the changes in patients' physical performance from preoperative to postoperative...... differences between the 2 groups on days 7 or 30 for fatigue, postural sway and QoL. DA treatment significantly (p

Oral sucrosomial iron versus intravenous iron in anemic cancer patients without iron deficiency receiving darbepoetin alfa: a pilot study.

Science.gov (United States)

Mafodda, Antonino; Giuffrida, D; Prestifilippo, A; Azzarello, D; Giannicola, R; Mare, M; Maisano, R

2017-09-01

Erythropoiesis-stimulating agents (ESAs) are often used in treatment of patients with chemotherapy-induced anemia. Many studies have demonstrated an improved hemoglobin (Hb) response when ESA is combined with intravenous iron supplementation and a higher effectiveness of intravenous iron over traditional oral iron formulations. A new formulation of oral sucrosomial iron featuring an increased bioavailability compared to traditional oral formulations has recently become available and could provide a valid alternative to those by intravenous (IV) route. Our study evaluated the performance of sucrosomial iron versus intravenous iron in increasing hemoglobin in anemic cancer patients receiving chemotherapy and darbepoetin alfa, as well as safety, need of transfusion, and quality of life (QoL). The present study considered a cohort of 64 patients with chemotherapy-related anemia (Hb >8 g/dL iron deficiency, scheduled to receive chemotherapy and darbepoetin. All patients received darbepoetin alfa 500 mcg once every 3 weeks and were randomly assigned to receive 8 weeks of IV ferric gluconate 125 mg weekly or oral sucrosomial iron 30 mg daily. The primary endpoint was to demonstrate the performance of oral sucrosomial iron in improving Hb response, compared to intravenous iron. The Hb response was defined as the Hb increase ≥2 g/dL from baseline or the attainment Hb ≥ 12 g/dL. There was no difference in the Hb response rate between the two treatment arms. Seventy one percent of patients treated with IV iron achieved an erythropoietic response, compared to 70% of patients treated with oral iron. By conventional criteria, this difference is considered to be not statistically significant. There were also no differences in the proportion of patients requiring red blood cell transfusions and changes in QoL. Sucrosomial oral iron was better tolerated. In cancer patients with chemotherapy-related anemia receiving darbepoetin alfa, sucrosomial oral iron provides

Interferon alfa-2b, colchicine, and benzathine penicillin versus colchicine and benzathine penicillin in Behçet's disease: a randomised trial.

Science.gov (United States)

Demiroglu, H; Ozcebe, O I; Barista, I; Dündar, S; Eldem, B

2000-02-19

Sight-threatening eye involvement is a serious complication of Behçet's disease. Extraocular complications such as arthritis, vascular occlusive disorders, mucocutaneous lesions, and central-nervous-system disease may lead to morbidity and even death. We designed a prospective study in newly diagnosed patients without previous eye disease to assess whether prevention of eye involvement and extraocular manifestations, and preservation of visual acuity are possible with combination treatments with and without interferon alfa-2b. Patients were randomly assigned 3 million units interferon alfa-2b subcutaneously every other day for the first 6 months plus 1.5 mg colchicine orally daily and 1.2 million units benzathine penicillin intramuscularly every 3 weeks (n=67), or colchicine and benzathine penicillin alone (n=68). The primary endpoint was visual-acuity loss. Analysis was by intention to treat. Significantly fewer patients who were treated with interferon had eye involvement than did patients who did not receive interferon (eight vs 27, relative risk 0.21 [95% CI 0.09-0.50], p<0.001). Ocular attack rate was 0.2 (SD 0.62) per year with interferon therapy and 1.02 (1.13) without interferon therapy (p=0.0001). Visual-acuity loss was significantly lower among patients treated with interferon than in those without interferon (two vs 13, relative risk 0.13 [95% CI 0.03-0.60], p=0.003). Arthritis episodes, vascular events, and mucocutaneous lesions were also less frequent in patients treated with interferon than in those not receiving interferon. No serious side-effects were reported. Therapy with interferon alfa-2b, colchicine, and benzathine penicillin seems to be an effective regimen in Behçet's disease for the prevention of recurrent eye attacks and extraocular complications, and for the protection of vision.

Coste-efectividad de drotrecogina alfa (activada en el tratamiento de la sepsis grave en España Cost-effectiveness of drotrecogin alpha [activated] in the treatment of severe sepsis in Spain

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José A Sacristán

2004-02-01

Full Text Available Introducción: El ensayo clínico PROWESS ha demostrado que el tratamiento con drotrecogina alfa (activada en pacientes con sepsis grave se asocia a una reducción del riesgo absoluto de muerte en comparación con el tratamiento estándar. El objetivo del estudio fue evaluar el coste-efectividad de drotrecogina alfa (activada, frente a tratamiento estándar, en el tratamiento de la sepsis grave en España. Pacientes y métodos: Se elaboró un modelo de análisis de decisión en el que se compararon los costes hasta el alta hospitalaria y la eficacia a largo plazo de drotrecogina alfa (activada frente a tratamiento estándar de pacientes con sepsis grave en España, desde la perspectiva del financiador de los servicios sanitarios. La mayoría de la información para realizar el modelo se obtuvo del ensayo clínico PROWESS. El análisis basal fue doble: a para todos los pacientes incluidos en el ensayo clínico PROWESS, y b para los pacientes con 2 o más fallos orgánicos. Las variables principales de valoración clínica fueron la reducción de la mortalidad hospitalaria y los años de vida ganados (AVG. El coste-efectividad se expresó como coste por AVG. Se realizó un análisis de sensibilidad utilizando tasas de descuento del 3 y el 5% para los AVG y modificando los patrones de atención sanitaria, los costes de la unidad de cuidados intensivos, la esperanza de vida según la comorbilidad inicial y la eficacia terapéutica de drotrecogina alfa (activada. Resultados: El tratamiento con drotrecogina alfa (activada se asoció a un descenso del riesgo absoluto de mortalidad hospitalaria del 6,0% (p = 0,005 cuando se incluyeron todos los pacientes del estudio PROWESS, y una reducción del 7,3% (p = 0,005 cuando sólo se incluyeron los pacientes con 2 o más fracasos orgánicos. El coste-efectividad de drotrecogina alfa (activada fue de 13.550 euros por AVG respecto a placebo cuando se analizó el total de pacientes, y de 9.800 euros por AVG en el

Enzymatic hydrolysis of pretreated Alfa fibers (Stipa tenacissima) using β-d-glucosidase and xylanase of Talaromyces thermophilus from solid-state fermentation.

Science.gov (United States)

Mallek-Fakhfakh, Hanen; Fakhfakh, Jawhar; Walha, Kamel; Hassairi, Hajer; Gargouri, Ali; Belghith, Hafedh

2017-10-01

This work aims at realizing an optimal hydrolysis of pretreated Alfa fibers (Stipa tenacissima) through the use of enzymes produced from Talaromyces thermophilus AX4, namely β-d-glucosidase and xylanase, by a solid state fermentation process of an agro-industrial waste (wheat bran supplemented with lactose). The carbon source was firstly selected and the optimal values of three other parameters were determined: substrate loading (10g), moisture content (85%) and production time (10days); which led to an optimized enzymatic juice. The outcome was then supplemented with cellulases of T. reesei and used to optimize the enzymatic saccharification of alkali-pretreated Alfa fibers (PAF). The maximum saccharification yield of 83.23% was achieved under optimized conditions (substrate concentration 3.7% (w/v), time 144h and enzyme loading of 0.8 FPU, 15U CMCase, 60U β-d-glucosidase and 125U xylanase).The structural modification of PAF due to enzymatic saccharification was supported by the changes of morphologic and chemical composition observed through macroscopic representation, FTIR and X-Ray analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

Epoetin alfa 40000 U once weekly and intravenous iron supply in solid tumor patients: early increase of hemoglobin level during chemotherapy

International Nuclear Information System (INIS)

Lalle, M.; Antimi, M.; Pistillucci, G.; D'Aprile, M.

2005-01-01

The objective of this observational study was the early evaluation of the impact, a week after the first administration of epoetin alfa 40000 U once weekly and i.v. dose of 62.5 mg sodium ferric gluconate for seven days in improving hemoglobin levels in cancer patients affected by mild/moderate or severe anemia during chemotherapy. Twenty patients affected by solid tumors who received epoetin alfa 40000 U once weekly and daily i.v. sodium ferric gluconate for one week were evaluated: 90% of the patients showed hemoglobin increase, with a median level of hemoglobin increase of 0.73 g/L from baseline, and 50% of them showing a hemoglobin increase > 1 gr/L. The treatment was well tolerated and no adverse event was observed. The early increase of hemoglobin level from baseline is interesting and suggestive for the possibility of achieving an adequate hemoglobin level with a short-term treatment. It is still necessary to further explore the real need of iron supplementation to maintain adequate erythropoiesis prior and during epoetin therapy

Artvin, Rize ve Trabzon’daki Doğal Kaynak Sularında Toplam Alfa, Toplam Beta Aktivite Değerlerinin Belirlenmesi ve Jeoistatistiksel Olarak Modellenmesi

Directory of Open Access Journals (Sweden)

Yaşar Kobya

2014-11-01

Full Text Available Trabzon, Rize ve Artvin yaklaşık 18000km2 alan üzerine kurulmuş ve yaklaşık 2 milyon insanın yaşadığı Türkiye’nin en kuzey doğusunda bulunan üç ildir. Bu üç ilde toplam 20 tane içme suyu kaynağından örnekler alındı ve bunların toplam alfa, toplam beta aktivite değerleri Berthold LB770 marka alfa, beta sayım cihazı kullanılarak belirlendi. Toplam alfa aktivite değerleri 1.7-114.2 Bq/L ve toplam beta aktivite değerleri ise 2.9-90.8 Bq/L aralıklarında ölçüldü. Bulunan bu değerler dünya sağlık örgütü (WHO’nün limit değerleri ve yapılmış diğer benzer çalışmalarla karşılaştırıldı. Elde edilen veriler için klasik istatistik hesaplamalar yapıldı. Ayrıca jeoistatistik analiz yöntemi kullanılarak eş-doz eğrileri oluşturuldu ve harita üzerinde gösterilerek bölge için bir risk haritası oluşturuldu.

Efeito da suplementação de linhaça, óleo de canola e vitamina E na dieta sobre as concentrações de ácidos graxos poliinsaturados em ovos de galinha Effect of dietary supplementation of flaxseed, canola oil and vitamin E upon polyunsaturated fatty acids in chicken eggs

Directory of Open Access Journals (Sweden)

M.C.G. Pita

2006-10-01

Full Text Available Estudou-se o efeito de diferentes fontes de ácidos graxos insaturados (óleo de canola e semente de linhaça, acrescidas de diferentes teores de alfa-tocoferol nas dietas de poedeiras, sobre a composição de ácidos graxos da gema do ovo foi estudado. Foram utilizadas 288 galinhas da linhagem Babcock que receberam dietas com 6% de óleo de canola, 20% de semente de linhaça moída ou 3% de óleo de canola e 10% de linhaça moída com teores de 0, 100 e 200UI/kg de alfa-tocoferol. As dietas com 20% de semente de linhaça proporcionaram teores mais elevados de ácidos graxos poliinsaturados no ovo com aumento, em particular, dos teores de ácido alfa-linolênico e EPA (ácido eicosapentaenóico e diminuição de ácido araquidônico na gema. Os teores de vitamina E contidos nas rações experimentais não determinaram alteração significativa na deposição dos diferentes ácidos graxos na gema dos ovos, exceto com relação aos ácidos graxos saturados.The effect of dietary sources of polyunsaturated fatty acids - canola oil and flaxseed - with different vitamin E supplementation on the fatty acid deposition into the eggs of 288 Babcock laying hens was investigated. Birds were fed diets containing 6% of canola oil, 20% of flaxseed or a combination of 3% of canola oil and 10% of flaxseed, enriched with 0, 100 or 200Ul of dl-alpha-tocopheril acetate. The inclusion of flaxseed in the diets increased the yolk polyunsaturated fatty acids, mainly alpha-linolenic acid and EPA (eicosapentaenoic acid. The concentration of alpha-tocopherol in the diet did not change the egg yolk, fatty acids deposition but changed the saturated fatty acids deposition.

Caracterização de atapulgita visando aplicação para reforço de materiais poloméricos

OpenAIRE

Costa, Antonio Carlos Silva da

2011-01-01

A atapulgita é um argilomineral do grupo das hormitas, que tem suas estruturas formadas por microcanais, que lhe conferem propriedades tecnológicas superiores a argilas classificadas industriais, esse grupo de argilas tem um leque de aplicações muito versátil, que vai de fluido de perfuração para poços de petróleo até aplicações na indústria farmacêutica. Tais propriedades podem ser melhoradas por ativação acida e/ou ativação térmica. A atapulgita quando ativada pode melhora...

Peginterferon alpha-2a is associated with higher sustained virological response than peginterferon alfa-2b in chronic hepatitis C: systematic review of randomized trials

DEFF Research Database (Denmark)

Awad, Tahany; Thorlund, Kristian; Hauser, Goran

2010-01-01

) is most effective. We performed a systematic review of head-to-head randomized trials to assess the benefits and harms of the two treatments. We searched the Cochrane Central Register of Controlled Trials, MEDLINE, EMBASE, and LILACS through July 2009. Using standardized forms, two reviewers independently...... extracted data from each eligible trial report. We statistically combined data using a random effects meta-analysis according to the intention-to-treat principle. We identified 12 randomized clinical trials, including 5,008 patients, that compared peginterferon alpha-2a plus ribavirin versus peginterferon...... alfa-2b plus ribavirin. Overall, peginterferon alpha-2a significantly increased the number of patients who achieved a sustained virological response (SVR) versus peginterferon alfa-2b (47% versus 41%; risk ratio 1.11, 95% confidence interval 1.04-1.19; P = 0.004 [eight trials]). Subgroup analyses...

Effects of lidocaine on lipopolysaccharide-induced synovitis in horses Efeitos da lidocaína em sinovite induzida por lipopolissacarídeo, em eqüinos

Directory of Open Access Journals (Sweden)

R.C. Campebell

2004-06-01

ísticas bioquímicas e celulares do líquido sinovial foram avaliadas nos mesmos tempos. A resposta inflamatória local e sistêmica foi mensurada pela concentração do TNF-alfa no soro e líquido sinovial. Observou-se aumento da concentração do TNF-alfa nas articulações injetadas com LPS às 3h no grupo A e de 1,30 às 3h no grupo B. Concluiu-se que o LPS induziu o processo inflamatório e que a lidocaína não inibiu nem atenuou a sinovite induzida pelo LPS, nem a síntese e liberação de TNF-alfa .

A Phase 3, multicenter, open-label, switchover trial to assess the safety and efficacy of taliglucerase alfa, a plant cell-expressed recombinant human glucocerebrosidase, in adult and pediatric patients with Gaucher disease previously treated with imiglucerase.

Science.gov (United States)

Pastores, Gregory M; Petakov, Milan; Giraldo, Pilar; Rosenbaum, Hanna; Szer, Jeffrey; Deegan, Patrick B; Amato, Dominick J; Mengel, Eugen; Tan, Ee Shien; Chertkoff, Raul; Brill-Almon, Einat; Zimran, Ari

2014-12-01

Taliglucerase alfa is a β-glucosidase enzyme replacement therapy (ERT) approved in the US and other countries for the treatment of Gaucher disease (GD) in adults and is approved in pediatric and adult patients in Australia and Canada. It is the first approved plant cell-expressed recombinant human protein. A Phase 3, multicenter, open-label, 9-month study assessed safety and efficacy of switching to taliglucerase alfa in adult and pediatric patients with GD treated with imiglucerase for at least the previous 2years. Patients with stable disease were offered taliglucerase alfa treatment using the same dose (9-60U/kg body weight) and regimen of administration (every 2weeks) as imiglucerase. This report summarizes results from 26 adult and 5 pediatric patients who participated in the trial. Disease parameters (spleen and liver volumes, hemoglobin concentration, platelet count, and biomarker levels) remained stable through 9months of treatment in adults and children following the switch from imiglucerase. All treatment-related adverse events were mild or moderate in severity and transient in nature. Exploratory parameters of linear growth and development showed positive outcomes in pediatric patients. These findings provide evidence of the efficacy and safety profile of taliglucerase alfa as an ERT for GD in patients previously treated with imiglucerase. This trial was registered at www.clinicaltrials.gov as # NCT00712348. Copyright © 2014 Elsevier Inc. All rights reserved.

Ansiedade na performance musical: tradução, adaptação e validação do Kenny Music Performance Anxiety Inventory (K-MPAI para a língua portuguesa

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Sérgio de Figueiredo Rocha

2011-01-01

Full Text Available CONTEXTO: A performance musical requer alto nível de habilidade em diversos parâmetros, como coordenação motora, atenção e memória, o que a torna particularmente suscetível aos estados de ansiedade. Pesquisas nessa área têm avançado com a introdução de instrumentos específicos para abordar a ansiedade na performance musical, como é o caso da Kenny Music Performance Anxiety Inventory (K-MPAI. OBJETIVOS: O presente estudo teve como objetivo traduzir, adaptar e validar a K-MPAI para a língua portuguesa. MÉTODOS: Após autorização da autora, a escala K-MPAI foi traduzida e validada. A escala em língua portuguesa foi aplicada a 218 músicos de ambos os sexos, amadores e profissionais. Para a validação concorrente, foi utilizado o Inventário de Ansiedade Traço-Estado (IDATE, versão validada na língua portuguesa da State Trait Anxiety Inventory (STAI. RESULTADOS: A análise da consistência interna apresentou alfa de Cronbach = 0,957 com p < 0,001, reprodutibilidade com p = 0,378 e validação concorrente com a IDATE com alfa de Cronbach = 0,642 e p < 0,001. CONCLUSÃO: O estudo permite considerar a amostra com graus de confiabilidade e reprodutibilidade elevados, o que traduz este estudo como provindo de uma amostra não tendenciada e replicável a outras populações. A validação concorrente entre a K-MPAI e a IDATE permite inferir que ambas as escalas são comparáveis na capacidade de medir os níveis de ansiedade em musicistas.

Diffusion Kurtosis Imaging Detects Microstructural Alterations in Brain of alfa-Synuclein Overexpressing Transgenic Mouse Model of Parkinson’s Disease: A Pilot Study

Czech Academy of Sciences Publication Activity Database

Khainar, A.; Latta, P.; Dražanová, Eva; Rudá-Kučerová, J.; Szabó, N.; Arab, A.; Hutter-Paier, B.; Havas, D.; Windisch, M.; Šulcová, A.; Starčuk jr., Zenon; Rektorová, I.

2015-01-01

Roč. 28, č. 4 (2015), 281-289 ISSN 1029-8428 R&D Projects: GA MŠk(CZ) LO1212; GA MŠk ED0017/01/01 Institutional support: RVO:68081731 Keywords : diffusion kurtosis imaging * alfa-Synuclein * TNWT-61 * Parkinson’s disease * Transgenic mice * TBSS Subject RIV: BH - Optics, Masers, Lasers Impact factor: 3.140, year: 2015

Intervalos de Confianza para el coeficiente alfa de Cronbach: aportes a la investigación pediátrica

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Tomás Caycho-Rodríguez

2017-06-01

Full Text Available En la actualidad, es cada vez más común encontrar estudios que brinden evidencias de la confiabilidad de las puntuaciones obtenidas, por instrumentos de medida utilizadas en investigaciones en ciencias de la salud. Este es el caso de la valiosa contribución de Aguilar-Navarro et al, quienes adaptaron la Escala de Actitudes hacia la Alimentación Infantil de Iowa (IIFAS en México, reportando la confiabilidad a través del alfa de Cronbach.

Efficacy of sofosbuvir plus ribavirin with or without peginterferon-alfa in patients with hepatitis C virus genotype 3 infection and treatment-experienced patients with cirrhosis and hepatitis C virus genotype 2 infection.

Science.gov (United States)

Foster, Graham R; Pianko, Stephen; Brown, Ashley; Forton, Daniel; Nahass, Ronald G; George, Jacob; Barnes, Eleanor; Brainard, Diana M; Massetto, Benedetta; Lin, Ming; Han, Bin; McHutchison, John G; Subramanian, G Mani; Cooper, Curtis; Agarwal, Kosh

2015-11-01

We conducted an open-label, randomized, phase 3 trial to determine the efficacy and safety of sofosbuvir and ribavirin, with and without peginterferon-alfa, in treatment-experienced patients with cirrhosis and hepatitis C virus (HCV) genotype 2 infection and treatment-naïve or treatment-experienced patients with HCV genotype 3 infection. The study was conducted at 80 sites in Europe, North America, Australia, and New Zealand Patients were randomly assigned (1:1:1) to groups given sofosbuvir and ribavirin for 16 weeks (n = 196); sofosbuvir and ribavirin for 24 weeks (n = 199); or sofosbuvir, peginterferon-alfa, and ribavirin for 12 weeks (n = 197). The primary end point was the percentage of patients with HCV RNA <15 IU/mL 12 weeks after stopping therapy (sustained virologic response [SVR12]). From October 2013 until April 2014, we enrolled and treated 592 patients-48 with genotype 2 HCV and compensated cirrhosis who had not achieved SVR with previous treatments and 544 with genotype 3 HCV (279 treatment-naïve and 265 previously treated). Overall, 219 patients (37%) had compensated cirrhosis. The last post-treatment week 12 patient visit was in January 2015. Rates of SVR12 among patients with genotype 2 HCV were 87% and 100%, for those receiving 16 and 24 weeks of sofosbuvir and ribavirin, respectively, and 94% for those receiving sofosbuvir, peginterferon, and ribavirin for 12 weeks. Rates of SVR12 among patients with genotype 3 HCV were 71% and 84% in those receiving 16 and 24 weeks of sofosbuvir and ribavirin, respectively, and 93% in those receiving sofosbuvir, peginterferon, and ribavirin. On-treatment virologic failure occurred in 3 patients with HCV genotype 3a receiving sofosbuvir and ribavirin for 24 weeks. The most common adverse events were fatigue, headache, insomnia, and nausea. Overall, 1% of patients discontinued treatment due to adverse events. Among patients with genotype 3 HCV infection, including a large proportion of treatment

PURIFICACIÓN Y CARACTERIZACIÓN DE alfa-AMILASA DE PENICILLIUM COMMUNE PRODUCIDA MEDIANTE FERMENTACIÓN EN FASE SÓLIDA

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Esperanza Espinel

2010-03-01

Full Text Available Este estudio reporta la purificación y caracterización parcial de una alfa-amilasa producida por Penicillium commune mediante fermentación en fase sólida, empleandoyuca blanca colombiana (Manihot esculenta Crantz como soporte. La enzima fue purificada por precipitación fraccionada con sulfato de amonio, cromatografía de intercambio aniónico (DEAE-Sephadex A-50, cromatografía de filtración por gel (Sephadex G-75 y cromatografía de intercambio catiónico (CM-Sephadex C-50 obteniendo una actividad específica final de 314,82 U/mg, un grado de purificación del orden de 62 y un rendimiento de 9%. La purificación hasta la homogeneidad fue confirmada por SDS-PAGE. El peso molecular estimado fue 35 kDa. La enzima mostró máxima actividad de hidrólisis de almidón soluble con pH 6,0, y estabilidad en un intervalo de pH de 5,0-7,0. La estabilidad térmica de la enzima se presentó en el intervalo de temperatura 0-50 °C y su temperatura óptima fue 70 °C. Los iones Ca2+,Ba2+ y Ag+ aumentaron significativamente la actividad de la enzima, siendo el ión Ca2+ el que tuvo el más alto poder activador. Cu2+ no alteró significativamente la actividad de la enzima, mientras que Li+ y Fe3+ la disminuyeron ligeramente (13%, y Co2+ y Hg2+ la disminuyeron 25% y 40% respectivamente. Los valores de Km y Vmáx fueron calculados usando la linealización de Lineweaver- Burk, con el resultado Km= 0,48 mg/mL y Vmáx = 5,85 micromol glucosa/min. Entre los principales productos de hidrólisis del almidón de yuca se encuentran la maltosa y la glucosa, este resultado proporciona evidencia de que la enzima es capaz de romper los enlaces glicosídicos alfa-1,4 del almidón, comportamiento característico de una alfa-amilasa.

Determinação sérica de haptoglobina, ceruloplasmina e alfa-glicoproteína ácida em cães com gastrenterite hemorrágica Determination of serum haptoglobin, ceruloplasmin and acid alpha-glycoprotein in dogs with haemorrhagic gastroenteritis

Directory of Open Access Journals (Sweden)

Márcia Mery Kogika

2003-06-01

Full Text Available As proteínas de fase aguda (PFA são proteínas plasmáticas, cujo estímulo à síntese ocorre de forma rápida e marcante em resposta à injúria tecidual. Estas proteínas permitem o diagnóstico de processos inflamatórios em animais com supressão ou depressão medular. Além disso, são úteis na monitorização da resolução tecidual de traumas ou inflamação e também na avaliação da resposta orgânica ao tratamento. Uma vez que a leucopenia é observada nos estádios iniciais da parvovirose canina, a dosagem das PFA pode permitir a avaliação do processo inflamatório sob estas condições. Considerando-se estas hipóteses, foram determinados os níveis séricos das PFA (haptoglobina, ceruloplasmina e alfa-glicoproteína ácida em 11 cães saudáveis e 11 cães leucopênicos com gastrenterite hemorrágica, com suspeita clínica de parvovirose canina. A avaliação estatística mostrou diferença significativa, com intervalo de confiabilidade de 99% (PAcute phase proteins (APP are serum proteins whose stimulus for the synthesis happens in a quick and intense manner in response to tissue injury. Those proteins allow the diagnosis of inflammatory process in animals with bone marrow depression and, also, they are useful in the follow up of tissue resolution of traumas or inflammation, as well as in the evaluation of the organic response of the treatment. As leukopenia is observed in the initial stage of the canine parvovirus infection, the dosage of APP can allow the evaluation of the inflammatory process under these conditions. According to these hypothesis, serum APP levels (haptoglobin, ceruloplasm and a-acid-glycoprotein in 11 healthy dogs and 11 leukopenic dogs with haemorrhagic gastroenteritis, clinically suspected of canine parvovirus infection, were measured. There was a significant difference, with confidence interval of 99% (P <0.01 for the haptoglobin (p <0.0064 and the acid alpha-glycoprotein (p <0.0042 and 95% (P <0.05 of

A first-year dornase alfa treatment impact on clinical parameters of patients with cystic fibrosis: the Brazilian cystic fibrosis multicenter study

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Tatiana Rozov

2013-12-01

Full Text Available OBJECTIVE: To describe the clinical impact of the first year treatment with dornase alfa, according to age groups, in a cohort of Brazilian Cystic Fibrosis (CF patients. METHODS: The data on 152 eligible patients, from 16 CF reference centers, that answered the medical questionnaires and performed laboratory tests at baseline (T0, and at six (T2 and 12 (T4 months after dornase alfa initiation, were analyzed. Three age groups were assessed: six to 11, 12 to 13, and >14 years. Pulmonary tests, airway microbiology, emergency room visits, hospitalizations, emergency and routine treatments were evaluated. Student's t-test, chi-square test and analysis of variance were used when appropriated. RESULTS: Routine treatments were based on respiratory physical therapy, regular exercises, pancreatic enzymes, vitamins, bronchodilators, corticosteroids, and antibiotics. In the six months prior the study (T0 phase, hospitalizations for pulmonary exacerbations occurred in 38.0, 10.0 and 61.4% in the three age groups, respectively. After one year of intervention, there was a significant reduction in the number of emergency room visits in the six to 11 years group. There were no significant changes in forced expiratory volume in one second (VEF1, in forced vital capacity (FVC, in oxygen saturation (SpO2, and in Tiffenau index for all age groups. A significant improvement in Shwachman-Kulczychi score was observed in the older group. In the last six months of therapy, chronic or intermittent colonization by P. aeruginosa was detected in 75.0, 71.4 and 62.5% of the studied groups, respectively, while S. aureus colonization was identified in 68.6, 66.6 and 41.9% of the cases. CONCLUSIONS: The treatment with dornase alfa promoted the maintenance of pulmonary function parameters and was associated with a significant reduction of emergency room visits due to pulmonary exacerbations in the six to 11 years age group, with better clinical scores in the >14 age group, one

Epoetin alfa improves anemia and anemia-related, patient-reported outcomes in patients with breast cancer receiving myelotoxic chemotherapy: Results of a european, multicenter, randomized, controlled trial

NARCIS (Netherlands)

P. Pronzato (Paolo); E. Cortesi (Enrico); C.C.D. van der Rijt (Carin); A. Bols (Alain); J.A. Moreno-Nogueira (José); C.F. de Oliveira; P. Barrett-Lee (Peter); P.J. Ostler (Peter); R. Rosso (Ricardo)

2010-01-01

textabstractPurpose. To evaluate the effects of epoetin alfa on patient- reported outcomes (PROs) in patients with breast cancer receiving myelotoxic chemotherapy. Materials and Methods. Women with hemoglobin concentrations ≤12.0 g/dl and an Eastern Cooperative Oncology Group performance status

Measurement of the proton-proton total cross section at √(s) = 7 TeV via elastic scattering with the ALFA sub-detector of ATLAS at the LHC

Energy Technology Data Exchange (ETDEWEB)

Kreutzfeldt, Kristof; Dueren, Michael; Heinz, Christian; Stenzel, Hasko [2. Physikalisches Institut, Universitaet Giessen (Germany)

2015-07-01

The ALFA (Absolute Luminosity for ATLAS) detector is one of the ATLAS forward detectors located about 240 m away from the interaction point in the LHC tunnel. ALFA is a scintillating fibre tracking detector housed in Roman pots and designed to measure elastic proton-proton scattering up to the smallest scattering angles. In 2011 data were taken at a centre-of-mass energy of √(s) = 7 TeV during a special low intensity fill of LHC with high β* = 90 m beam optics. Results of the measurement of the differential elastic cross section dσ/dt, the total cross section σ{sub tot}, the nuclear slope B and other derived quantities are presented in this talk. In the analysis the luminosity determination, the fine tuning of beam optics parameters and a data-driven method to determine the reconstruction efficiency are emphasized.

A serine palmitoyltransferase inhibitor blocks hepatitis C virus replication in human hepatocytes.

Science.gov (United States)

Katsume, Asao; Tokunaga, Yuko; Hirata, Yuichi; Munakata, Tsubasa; Saito, Makoto; Hayashi, Hitohisa; Okamoto, Koichi; Ohmori, Yusuke; Kusanagi, Isamu; Fujiwara, Shinya; Tsukuda, Takuo; Aoki, Yuko; Klumpp, Klaus; Tsukiyama-Kohara, Kyoko; El-Gohary, Ahmed; Sudoh, Masayuki; Kohara, Michinori

2013-10-01

Host cell lipid rafts form a scaffold required for replication of hepatitis C virus (HCV). Serine palmitoyltransferases (SPTs) produce sphingolipids, which are essential components of the lipid rafts that associate with HCV nonstructural proteins. Prevention of the de novo synthesis of sphingolipids by an SPT inhibitor disrupts the HCV replication complex and thereby inhibits HCV replication. We investigated the ability of the SPT inhibitor NA808 to prevent HCV replication in cells and mice. We tested the ability of NA808 to inhibit SPT's enzymatic activity in FLR3-1 replicon cells. We used a replicon system to select for HCV variants that became resistant to NA808 at concentrations 4- to 6-fold the 50% inhibitory concentration, after 14 rounds of cell passage. We assessed the ability of NA808 or telaprevir to inhibit replication of HCV genotypes 1a, 1b, 2a, 3a, and 4a in mice with humanized livers (transplanted with human hepatocytes). NA808 was injected intravenously, with or without pegylated interferon alfa-2a and HCV polymerase and/or protease inhibitors. NA808 prevented HCV replication via noncompetitive inhibition of SPT; no resistance mutations developed. NA808 prevented replication of all HCV genotypes tested in mice with humanized livers. Intravenous NA808 significantly reduced viral load in the mice and had synergistic effects with pegylated interferon alfa-2a and HCV polymerase and protease inhibitors. The SPT inhibitor NA808 prevents replication of HCV genotypes 1a, 1b, 2a, 3a, and 4a in cultured hepatocytes and in mice with humanized livers. It might be developed for treatment of HCV infection or used in combination with pegylated interferon alfa-2a or HCV polymerase or protease inhibitors. Copyright © 2013 AGA Institute. Published by Elsevier Inc. All rights reserved.

INFLUÊNCIA DOS STAKEHOLDERS NA ADOÇÃO DE ESTRATÉGIAS DE MARKETING VERDE

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Benny Kramer Costa

2012-01-01

Full Text Available Este artigo tem como objetivo conhecer de que forma os stakeholders influenciam na adoção de estratégias de marketing verde sob a ótica da empresa Alfa, uma indústria moveleira situada no Rio Grande do Norte, Brasil. A metodologia utilizada teve uma abordagem qualitativa e utiliza o método do estudo de caso exploratóriodescritivo como modelo formal e sistemático do estudo científico. Segue as proposições teórico-conceituais de Polonsky (1995, Michell, Angle e Wood (1997 e Frooman (1999 como referências básicas que identifica e avalia o grau de importância dos stakeholders relevantes, mostra suas expectativas e necessidades e descreve as táticas utilizadas pelas empresas para a implantação de estratégias de marketing verde. O estudo descreve a realidade de uma indústria moveleira do Rio Grande do Norte, Brasil e mostra sua filosofia e contexto; identifica os stakeholders presentes, analisa o grau de importância de cada grupo relevante e mostra suas necessidades e expectativas e por fim, constata as mudanças ocorridas na organização na implantação de estratégias de marketing verde. A partir dos resultados obtidos conclui que os stakeholders são levados em consideração na adoção de estratégias de marketing verde, mesmo a empresa não possuindo a devida percepção estratégica necessária para avançar na adoção da filosofia do marketing verde. Este estudo de caso explora conhecimentos que podem ser apropriados e adequados por empresas de pequeno porte que atuem nesse segmento-tendência estratégico do marketing verde.

Factor de necrosis tumoral alfa en una población infanto-juvenil con sobrepeso

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Teresita del R. Carrizo

2013-08-01

Full Text Available El sobrepeso infantil está asociado a sobrepeso/obesidad en la edad adulta. El tejido adiposo en obesos produce una cantidad incrementada de citoquinas proinflamatorias como el factor de necrosis tumoral alfa (TNF-a, ejerciendo un efecto deletéreo sobre la función vascular. El objetivo de este trabajo fue evaluar niveles de TNF-a en una población infantojuvenil con sobrepeso y su relación con otras variables. Se estudiaron 30 niños con sobrepeso (12 varones de edades entre 8-13 años, se midió circunferencia de cintura (CC e índice de masa corporal (IMC y fueron comparados con 20 controles de edad y sexo semejantes. Se consideró criterio de inclusión un IMC = 85 < 95 percentilo para edad y sexo. En ambos grupos se determinó: glucemia en ayunas (método glucosa oxidasa, insulina plasmática (ECLIA, fibrinógeno (Fg, método de Clauss, proteína C reactiva ultrasensible (uPCR, método inmunoturbidimétrico, TNF-a (ELISA, perfil lipídico (métodos enzimáticos, eritrosedimentación y se calculó el índice HOMA. Los datos se expresaron como mediana y rango intercuartil y con el coeficiente de Spearman se investigaron las correlaciones entre variables, considerándose significativo un p < 0.05. Los niveles de TNF-a fueron mayores en los sujetos con sobrepeso [15.4 (13.2-24.0 vs. 12.7 (11.2-14.8 pg/ml; p = 0.028]. También resultaron más elevados los valores de Fg, insulina plasmática, índice HOMA, uPCR y triglicéridos. El TNF-a se correlacionó con la CC (r = 0.654; p = 0.021. Los niveles elevados de TNF-a, uPCR y Fg encontrados confirman un estado proinflamatorio asociado a obesidad abdominal en la población estudiada.

Comparable pharmacokinetics and pharmacodynamics of two epoetin alfa formulations Eporon® and Eprex® following a single subcutaneous administration in healthy male volunteers

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Yoon S

2017-10-01

Full Text Available Sumin Yoon,1 Su-jin Rhee,1 Sun Ju Heo,2 Tae Young Oh,2 Seo Hyun Yoon,1 Joo-Youn Cho,1 SeungHwan Lee,1,3 Kyung-Sang Yu1,3 1Department of Clinical Pharmacology and Therapeutics, Seoul National University College of Medicine and Hospital, Seoul, 2Dong-A ST Co. Ltd., Seoul, 3Clinical Trials Center, Seoul National University Hospital, Seoul, Republic of Korea Purpose: This study aimed to assess and compare the pharmacokinetic (PK and pharmacodynamic (PD properties following a single subcutaneous injection of epoetin alfa (Eporon® with those of the comparator (Eprex® in healthy male subjects. Subjects and methods: A randomized, double-blind, two-sequence, crossover study was conducted. Subjects were randomly assigned to receive a single dose, that is, 4,000 IU, of the test or comparator epoetin alfa. After 4 weeks, all subjects received the alternative formulation. The primary PK parameters, maximum observed concentration (Cmax and area under the curve extrapolated to infinity (AUCinf, were calculated with the serum erythropoietin (EPO concentrations from blood samples collected for 144 h after dosing. The reticulocyte, hematocrit, hemoglobin and red blood cell counts were measured up to 312 h as PD markers. The primary PD parameters, maximum observed effect (Emax and area under the effect curve (AUEC, were obtained from the baseline-corrected reticulocyte count. The serum EPO concentration and the reticulocyte count were used to assess the concentration–response relationship. The tolerability and immunogenicity profiles were assessed together. Results: Forty-two subjects completed the study. The mean EPO concentration–time profiles were comparable between the two formulations. The geometric mean ratios (90% CI of the Cmax and AUCinf were 0.908 (0.843–0.978 and 1.049 (0.999–1.101, respectively, both of which were within the regulatory range of 0.80–1.25. Additionally, the PD and tolerability profiles were similar between the two

Moldagem em prótese fixa: confecção do casquete de moldagem

OpenAIRE

Aloísio Oro Spazzin; Manuel Tomás Borges Radaelli; Leonardo Federizzi; Gabriel Kalil Rocha Pereira; Ataís Bacchi; Rodrigo Alessandretti

2017-01-01

O objetivo do artigo foi descrever a técnica de confecção de uma moldeira individual para moldagem de preparo total para coroa, também conhecida como casquete de moldagem, além de discutir fatores e princípios envolvidos no tema. Na presente técnica, é obtido um modelo de gesso da região do preparo e um alívio em cera é realizado em suas paredes axiais. Após a aplicação de um isolante, o casquete é confeccionado utilizando pó e líquido de resina acrílica ativada quimicamente indicada para con...

Development of solar energy education IASEE-Argentina, Arquisur and Alfa-built

International Nuclear Information System (INIS)

Schiller, S. de; Evans, J.M.

1997-01-01

Three educational experiences are presented in this paper on the development of national and regional networks to incorporate renewable energy in curricular programmes. IASEE-Argentina, established in 1990 as the National Section of IASEE, the International Association for Solar Energy Education, was designated in 1993 as the Education Working Group of ASADES, the Argentine Association of Solar Energy. It holds annual meetings to coincide with the ASADES conference. Arquisur is a regional network established by Architectural Faculties of State Universities within the Mercosur Region, covering Argentina, southern Brazil, Paraguay and Uruguay, to promote exchange and development between the 19 faculties involved. A Working Group set up by Arquisur with members from four countries developed the programme for short post-graduate courses on bioclimatic design and rational energy use in buildings, which has been given in Argentina and Uruguay. Alfa-Built, a project supported by the European Union for promoting academic exchange in the field of energy efficient building is also introduced. This paper presents the development and initial results of these educational experiences. (author)

EFEITO DA ADIÇÃO DE NUTRIENTES NA FERMENTAÇÃO ALCOÓLICA DE MEIO DE CULTIVO ELABORADO COM FÉCULA DE MANDIOCA

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EDWIL APARECIDA LUCCA GATTAS

2007-01-01

Full Text Available O objetivo do trabalho foi verificar o efeito da adição de sulfato de amônio ou uréia em meios contendo KH2PO4, extrato de levedura e fécula de mandioca dextrinizada na velocidade de produção de etanol em processo de sacarificação – fermentação simultâneas. Ficou demonstrada a necessidade de suplementação do meio de cultivo com nitrogênio, podendo ser utilizados com este fim tanto o sulfato de amônio como a uréia. Nos meios enriquecidos com nutrientes houve aumento na velocidade de fermentação de 41%. Na etapa da sacarificação levada a efeito na temperatura ótima da enzima, observou-se que é indiferente empregar-se as amiloglicosidases comercializadas pela Novo Industri S.A. (Spiritamylase e SAN 200 OL ou pela Pfizer, sempre precedidas pela liquefação com alfa-amilase da Okochi Ltda.

Influência do desenvolvimento etário e da suplementação com vitamina E (acetato de DL-alfa-tocoferol no metabolismo oxidativo dos neutrófilos de bovinos da raça Holandesa (Bos taurus

Directory of Open Access Journals (Sweden)

Joselito Nunes Costa

2004-10-01

Full Text Available O presente trabalho teve por objetivo analisar a influência do desenvolvimento etário e da suplementação com acetato de DL-alfa-tocoferol sobre o metabolismo oxidativo de neutrófilos, em bovinos da raça holandesa, no período do nascimento até os 150 dias de idade. Foram utilizados 20 bezerros divididos em dois grupos de dez animais. Os animais do grupo Tratamento receberam 2000UI de acetato de DL-alfa-tocoferol, por via intramuscular, ao nascimento, aos 15, 30, 60, 90 e 120 dias de idade, sendo o outro o grupo Controle, que não recebeu qualquer suplementação. Em ambos os grupos, o metabolismo oxidativo dos neutrófilos demonstrou pouca atividade durante os primeiros 60 dias de vida, sendo indicativo da ineficiência deste importante mecanismo bactericida. Não foi observado efeito significativo da administração do acetato de DL-alfa-tocoferol sobre o metabolismo oxidativo de neutrófilos.

ALGLUCOSIDASE ALFA — A NEW STAGE IN THE THERAPY OF INFANTILE POMPE DISEASE

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E. N. Basargina

2014-01-01

Full Text Available Pompe disease is a rare severe hereditary disease caused by excessive glycogen storage in organs and target tissues due to the acid α-glucosidase gene mutation. Infantile and adult Pompe disease is characterized by involvement of cardiovascular, respiratory and muscular systems in the pathological process. The only specific method of treating Pompe disease is enzyme replacement therapy (intravenous administration of recombinant human acid glucosidase, the effectiveness whereof depends on the time the therapy started. Since such a therapy was introduced into practice, Pompe disease mortality decreased by 79%. 6 children with infantile Pompe disease were observed and treated at the cardiovascular care unit of the Scientific Center of Children’s Health in 2011‑2014. The article presents a clinical case demonstrating capabilities of diagnosing infantile Pompe disease in Russia and effective application of alglucosidase alfa in 4-month-old child. 

Surface displaced alfa-enolase of Lactobacillus plantarum is a fibronectin binding protein

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Muscariello Lidia

2009-02-01

Full Text Available Abstract Background Lactic acid bacteria of the genus Lactobacillus and Bifidobacterium are one of the most important health promoting groups of the human intestinal microbiota. Their protective role within the gut consists in out competing invading pathogens for ecological niches and metabolic substrates. Among the features necessary to provide health benefits, commensal microorganisms must have the ability to adhere to human intestinal cells and consequently to colonize the gut. Studies on mechanisms mediating adhesion of lactobacilli to human intestinal cells showed that factors involved in the interaction vary mostly among different species and strains, mainly regarding interaction between bacterial adhesins and extracellular matrix or mucus proteins. We have investigated the adhesive properties of Lactobacillus plantarum, a member of the human microbiota of healthy individuals. Results We show the identification of a Lactobacillus plantarum LM3 cell surface protein (48 kDa, which specifically binds to human fibronectin (Fn, an extracellular matrix protein. By means of mass spectrometric analysis this protein was identified as the product of the L. plantarum enoA1 gene, coding the EnoA1 alfa-enolase. Surface localization of EnoA1 was proved by immune electron microscopy. In the mutant strain LM3-CC1, carrying the enoA1 null mutation, the 48 kDa adhesin was not anymore detectable neither by anti-enolase Western blot nor by Fn-overlay immunoblotting assay. Moreover, by an adhesion assay we show that LM3-CC1 cells bind to fibronectin-coated surfaces less efficiently than wild type cells, thus demonstrating the significance of the surface displaced EnoA1 protein for the L. plantarum LM3 adhesion to fibronectin. Conclusion Adhesion to host tissues represents a crucial early step in the colonization process of either pathogens or commensal bacteria. We demonstrated the involvement of the L. plantarum Eno A1 alfa-enolase in Fn-binding, by studying

Adrenergic system participation on LH secretion in pre-pubertal Nelore heifers Participação do sistema adrenérgico na secreção de LH, em novilhas nelores

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Guilherme Paula Nogueira

2008-12-01

Full Text Available The aim of this study was to evaluate the response of clonidine administration (alfa-adrenoceptor agonist, 10g/kg body weight, i.v. and blood collected every 15 min for 4 h thereafter on luteinizing hormone (LH secretion in B. indicus pre-pubertal heifers at 8, 12 e 15 months of age. LH was quantified by RIA, sensitivity (0.05 ng/mL and coefficient of variation (20.39%. Alfa-adrenoceptor action (clonidine reduced (P<0,05 basal LH and time to greatest peak occurrence at 15 months-old and increased (P>0,05 peak total area and area of highest peak of LH secretion to 8 months-old. The results suggest reduced hypothalamic sensibility by gonadal steroid in pre-pubertal Nelore heifer maturation sexual and clonidine neurotransmitter participation, either stimulating or inhibiting LH secretion.Objetivou-se com este trabalho investigar a variação na secreção do hormônio luteinizante (LH em resposta ao tratamento com clonidina (agonista alfa-adrenérgico, 10µg/kg, i.v., amostras 15 min por 4h, em novilhas da raça Nelore pré-púberes aos 8, 12 e 15 meses de idade. A concentração de LH foi quantificada por radioimunoensaio, sensibilidade de 0,05 ng/mL e coeficiente de variação de 20,39%. A ativação de receptores alfa-adrenérgicos por intermédio da clonidina diminuiu (P<0,05 a concentração de LH e o tempo necessário para o aparecimento do maior pico de LH aos 15 meses de idade e aumentou (P>0,05 a área total e área do maior pico de secreção de LH aos 8 meses de idade. Os resultados indicam diminuição da sensibilidade do hipotálamo aos esteróides gonadais durante o processo de maturação sexual nas novilhas da raça Nelore e a participação da clonidina como neurotransmissora.

An Alpha spectrometer for measuring radon daughter individual activity concentration; Spettrometro Alfa per la misura delle concentrazioni individuali in attivita' della progenie del radon

Energy Technology Data Exchange (ETDEWEB)

Berico, M.; Formignani, M. [ENEA, Div. Protezione dell' Uomo e degli Ecosistemi, Centro Ricerche E. Clementel, Bologna (Italy); Mariotti, F. [Bologna Univ., Bologna (Italy). Dipt. di Fisica

2001-07-01

In the frame of the program of the Institute for Radiation Protection of ENEA, related to the evaluation of dose from radon and thoron progeny, an alpha spectrometer for the continuous air monitoring (CAM type) of radon and thoron has been realized. The constructive characteristics of the device are here presented together with energy and efficiency calibration. The device allows, by means of a screen type diffusion battery and a filter, to determinate the single radioactivity of each radionuclide of the progeny selecting them in relation to their diffusive behaviour (dichotomous particle size selection). The three-count filter method has been employed to measure the concentrations of {sup 218}Po, {sup 214}Pb and {sup 214}Bi in air. Radon and thoron effective doses using a dosimetric, instead of an epidemiologic approach, will be then evaluated. [Italian] Presso l'Istituto per la Radioprotezione, nell'ambito del programma di valutazione di dose da radon e' stato progettato e realizzato uno spettrometro alfa per il monitoraggio continuo in aria (CAM) della progenie del radon e del toron. Le caratteristiche costruttive dello strumento permettono, tramite l'utilizzo di batterie a diffusione a reti, di determinare l'attivita' individuale della progenie per diverse dimensioni granulometriche in particolare per la frazione attaccata e non al particolato amosferico con un taglio granulometrico di qualche nanometro. E' stato inoltre applicato un metodo spettrometrico a tre conteggi per il calcolo delle concentrazioni individuali della progenie del radon, {sup 218}Po, {sup 214}Pb and {sup 214}Bi, effettuando un conteggio alfa di {sup 218}Po e due conteggi alfa di {sup 214}Po. Tale informazione consentira' una valutazione della dose di radon utilizzando il modello dosimetrico in alternativa a quello epidemiologico.

Onset of Type 1 Diabetes Mellitus During Pegylated-interferon Alfa and Ribavirin Therapy for Chronic Hepatitis C Virus Infection

Science.gov (United States)

Ranganathan, Raghini; Janarthanan, Krishnaveni; Rajasekaran, Senthilkumar

2012-01-01

A 16-year-old female was treated with pegylated-interferon (PEG-IFN) alfa (a)-2b and ribavirin combination therapy for chronic hepatitis C virus (HCV) infection. She attained rapid virological response. She presented with diabetic ketoacidosis after 41 weeks of therapy. Anti-glutamic acid decarboxylase antibodies and islet cell antibodies were negative. Her fasting serum C-peptide level was <0.1 ng/mL, and the treatment course was completed. This case underlines the importance of periodic plasma glucose monitoring in patients during and after PEG-IFN and ribavirin therapy. PMID:25755410

Seguridad de la terapia de interferón alfa 2b recombinante más ribavirina en la hepatitis crónica C

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Yoan Antonio Sánchez Rodríguez

2011-03-01

Full Text Available INTRODUCCIÓN: la hepatitis crónica C ha adquirido rango de pandemia. El virus de la hepatitis C se ha convertido en la causa principal de hepatitis crónica, cirrosis hepática, hepatocarcinoma, y trasplante de hígado a nivel mundial. OBJETIVO: identificar los efectos adversos asociados a la terapia combinada interferón alfa 2b recombinante más ribavirina durante la evolución del tratamiento y hasta 8 semanas después de finalizado, así como los principales efectos asociados a salidas temporales o definitivas de esta terapia. MÉTODOS: estudio de farmacovigilancia cuya serie estuvo conformada por 122 pacientes con hepatitis crónica C atendidos en el Instituto de Gastroenterología desde mayo de 2001 hasta mayo de 2006. Se utilizó interferón alfa 2b recombinante (3 millones de unidades 3 veces por semana más ribavirina (1 000 o 1 200 mg diarios en dependencia del peso corporal durante 48 semanas. RESULTADOS: el 88,5 % del total de casos presentó efectos adversos; de ellos el 79,5 % correspondió al síndrome seudogripal, seguido de manifestaciones hematológicas, neuropsiquiátricas, gastrointestinales, entre otras menos frecuentes. El 6,6 % de la serie presentó salidas temporales del tratamiento por efecto adverso distinto de la anemia y 4 pacientes, salidas definitivas del estudio, tres por anemia hemolítica severa y uno con hipertiroidismo no controlable. CONCLUSIONES: la terapia combinada interferón alfa 2b recombinante más ribavirina resulta segura, donde el mayor número de casos presentó síndrome seudogripal como efecto adverso más frecuente. Las manifestaciones hematológicas asociadas a las salidas definitivas del estudio permitieron recomendar seguimiento estricto de la hemoglobina y profundizar en el diagnóstico y tratamiento de los principales efectos adversos presentes en otros sistemas y asociados a esta terapia.

Alfa-amilase em frangos de corte: efeitos do balanço eletrolítico e do nível protéico da dieta Alpha-amylase in broiler chickens: effects of electrolytic balance and dietary protein level

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Marcela Piedade Monteiro

2006-06-01

Full Text Available Um experimento foi conduzido com pintos de corte macho para o estudo dos efeitos dos níveis de 20 e 23% de PB combinados com 0, 50, 100, 150, 200, 250 mEq/kg de balanço dietético eletrolítico (BDE sobre a atividade da alfa-amilase pancreática de frangos de corte de 1 a 21 dias de idade. O delineamento utilizado foi o inteiramente casualizado. Dietas e água foram fornecidas ad libitum. Aos 1, 7, 14 e 21 dias, três aves de cada tratamento foram sacrificadas por deslocamento cervical para remoção do pâncreas, o qual foi removido, homogeneizado, congelado em nitrogênio líquido e liofilizado. Uma alíquota de cada amostra foi solubilizada em água deionizada e centrifugada a 7500 x g por 3 minutos a 4ºC, para determinação da atividade da alfa-amilase no sobrenadante. Aves alimentadas com 20% de PB apresentaram atividades específicas (U/mg de proteína superiores à daquelas que receberam 23%, exceto para os níveis de BDE de 100 a 200 mEq/kg, aos 14 dias. Observou-se tendência de aumento da atividade específica dos 7 aos 14 dias e redução dos 14 aos 21 dias. Para maior atividade específica de alfa-amilase, recomendam-se dietas com 20% de PB e 200 mEq/kg de BDE na fase pré-inicial e dietas com 20% de PB e 135 a 250 mEq/kg de BDE para frangos de corte dos 8 aos 21 dias de idade.An experiment was conducted with male broiler chicks to study the effects of 20 and 23% of crude protein (CP combined with 0, 50, 100, 150, 200, 250 mEq/kg of dietary electrolytic balance (DEB on the pancreatic alpha-amylase activity from 1 to 21 days. A completely randomized factorial design was used. Feed and water were supplied ad libitum. At days 1, 7, 14 and 21, three birds from each treatment were sacrificed by cervical dislocation, pancreas were removed, homogeneized and frozen in liquid nitrogen which were then freeze-dried. An aliquot of each sample was solubilized with deionized water and centrifuged at 7500 x g for three minutes at 4º

CONFIRMAÇÃO DA IDENTIDADE DA alfa-CRIPTOXANTINA E INCIDÊNCIA DE CAROTENÓIDES MINORITÁRIOS PROVITAMÍNICOS A EM VERDURAS FOLHOSAS VERDES CONFIRMATION OF THE IDENTITY OF alpha-CRYPTOXANTHIN AND INCIDENCE OF MINOR PROVITAMIN A CAROTENOIDS IN GREEN LEAFY VEGETABLES

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Adriana Z. MERCADANTE

2001-08-01

Full Text Available Numerosos trabalhos comprovaram que os carotenóides principais de folhas verdes são invariavelmente luteína, beta-caroteno, violaxantina e neoxantina. No entanto, há discordância em torno dos carotenóides minoritários. Portanto, a espectrometria de massas por impacto de elétrons e cromatografia líquida de alta eficiência com detector de arranjo de diodos foram utilizados para confirmar a identidade de carotenóides minoritários com atividade provitamínica A em verduras folhosas brasileiras. Os carotenóides pró-vitamínicos A, incluindo os isômeros cis e trans de beta-caroteno, foram separados em coluna de C18 polimérica, Vydac 201TP54, com metanol/água (98:2 como fase móvel. Os espectros UV-visível e de massas confirmaram o carotenóide monoidroxilado como sendo alfa-criptoxantina e não beta-criptoxantina como aponta a literatura internacional. Todas as onze folhas analisadas (agrião, alface crespa, alface lisa, almeirão, caruru, chicória, couve, espinafre, rúcula, salsinha e taioba apresentaram alfa-criptoxantina, 13-cis-beta-caroteno e 9-cis-beta-caroteno, enquanto que alfa-caroteno foi encontrado em apenas quatro folhas (caruru, couve, salsinha e taioba.The main carotenoids from green leafy vegetables have been consistently found to be lutein, beta-carotene, violaxanthin and neoxanthin. However, there is a controversy about the identity of minor carotenoids. Therefore, electron impact mass spectrometry and high performance liquid chromatography with a diode array detector were used in order to confirm the identity of the minor provitamin A carotenoids in Brazilian green leaves. The provitamin A carotenoids, including the cis and trans isomers of beta-carotene, were separated on a polymeric C18 column, Vydac 201TP54, with MeOH/H2O (98:2 as mobile phase. The UV-visible and mass spectra confirmed that the monohydroxy carotenoid present in Brazilian green leafy vegetable to be alpha-cryptoxanthin, and not beta

JAK-inhibitor tofacitinib suppresses interferon alfa production by plasmacytoid dendritic cells and inhibits arthrogenic and antiviral effects of interferon alfa.

Science.gov (United States)

Boor, Patrick P C; de Ruiter, Petra E; Asmawidjaja, Patrick S; Lubberts, Erik; van der Laan, Luc J W; Kwekkeboom, Jaap

2017-10-01

Tofacitinib is an oral Janus kinase inhibitor that is effective for the treatment of rheumatoid arthritis and shows encouraging therapeutic effects in several other autoimmune diseases. A prominent adverse effect of tofacitinib therapy is the increased risk of viral infections. Despite its advanced stage of clinical development, the modes of action that mediate the beneficial and adverse effects of tofacitinib in autoimmune diseases remain unclear. Interferon alfa (IFNα) produced by plasmacytoid dendritic cells (PDCs) is critically involved in the pathogenesis of many systemic autoimmune diseases and in immunity to viral infections. Using in vitro culture models with human cells, we studied the effects of tofacitinib on PDC survival and IFNα production, and on arthrogenic and antiviral effects of IFNα. Tofacitinib inhibited the expression of antiapoptotic BCL-A1 and BCL-XL in human PDC and induced PDC apoptosis. TLR7 stimulation upregulated the levels of antiapoptotic Bcl-2 family members and prevented the induction of PDC apoptosis by tofacitinib. However, tofacitinib robustly inhibited the production of IFNα by toll like receptor-stimulated PDC. In addition, tofacitinib profoundly suppressed IFNα-induced upregulation of TLR3 on synovial fibroblasts, thereby inhibiting their cytokine and protease production in response to TLR3 ligation. Finally, tofacitinib counteracted the suppressive effects of IFNα on viral replication. Tofacitinib inhibits PDC survival and IFNα production and suppresses arthrogenic and antiviral effects of IFNα signaling. Inhibition of the IFNα pathway at 2 levels may contribute to the beneficial effects of tofacitinib in autoimmune diseases and explain the increased viral infection rates observed during tofacitinib treatment. Copyright © 2016 Elsevier Inc. All rights reserved.

Estudo de associação genética entre fator de necrose tumoral alfa e sepse e choque séptico em pacientes pediátricos tailandeses

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Suwannee Phumeetham

2012-10-01

Full Text Available OBJETIVOS: Avaliar a associação entre o polimorfismo genético do fator de necrose tumoral alfa (TNF-α e o desenvolvimento de sepse e choque séptico em pacientes pediátricos tailandeses e investigar o impacto clínico de polimorfismos do TNF-α nessa população. MÉTODOS: Para a realização deste estudo de associação genética, foram analisados prospectivamente pacientes pediátricos (idade < 18 anos com sepse clínica/choque séptico. Todos os dados foram coletados por especialistas em terapia intensiva pediátrica e as análises genéticas foram realizadas em um laboratório central. Um polimorfismo de nucleotídeo único [single nucleotide polymorphism (SNP], localizado na região promotora 5' na posição -308, foi genotipado e os resultados foram associados a fenótipos clínicos. RESULTADOS: Foram investigados 167 indivíduos tailandeses, dos quais 66 eram pacientes pediátricos com sepse/choque séptico e 101 eram controles saudáveis. Curiosamente, não foi possível identificar associação entre sepse e o polimorfismo -308 (G→A, um dos principais SNPs anteriormente associado à sepse em várias populações caucasianas, visto que não houve diferença de frequência entre casos e controles. CONCLUSÕES: Neste estudo, o principal polimorfismo do TNF-α -308 não esteve associado à sepse clínica/choque séptico na população tailandesa. Essa informação é importante para futuras análises que busquem identificar a função do TNF-α como risco genético para o desenvolvimento de imunopatologia subjacente a várias doenças no continente asiático.

Ovarian response to 150 µg corifollitropin alfa in a GnRH-antagonist multiple-dose protocol: a prospective cohort study.

Science.gov (United States)

Lerman, Tamara; Depenbusch, Marion; Schultze-Mosgau, Askan; von Otte, Soeren; Scheinhardt, Markus; Koenig, Inke; Kamischke, Axel; Macek, Milan; Schwennicke, Arne; Segerer, Sabine; Griesinger, Georg

2017-05-01

The incidence of low (18 oocytes) ovarian response to 150 µg corifollitropin alfa in relation to anti-Müllerian hormone (AMH) and other biomarkers was studied in a multi-centre (n = 5), multi-national, prospective, investigator-initiated, observational cohort study. Infertile women (n = 212), body weight >60 kg, underwent controlled ovarian stimulation in a gonadotrophin-releasing hormone-antagonist multiple-dose protocol. Demographic, sonographic and endocrine parameters were prospectively assessed on cycle day 2 or 3 of a spontaneous menstruation before the administration of 150 µg corifollitropin alfa. Serum AMH showed the best correlation with the number of oocytes obtained among all predictor variables. In receiver-operating characteristic analysis, AMH at a threshold of 0.91 ng/ml showed a sensitivity of 82.4%, specificity of 82.4%, positive predictive value 52.9%and negative predictive value 95.1% for predicting low response (area under the curve [AUC], 95% CI; P-value: 0.853, 0.769-0.936; <0.0001). For predicting high response, the optimal threshold for AMH was 2.58 ng/ml, relating to a sensitivity of 80.0%, specificity 82.1%, positive predictive value 42.5% and negative predictive value 96.1% (AUC, 95% CI; P-value: 0.871, 0.787-0.955; <0.0001). In conclusion, patients with serum AMH concentrations between approximately 0.9 and 2.6 ng/ml were unlikely to show extremes of response. Copyright © 2017. Published by Elsevier Ltd.

Structural and functional assessment of the interaction between focal adhesion kinase and sarcomeric myosin

OpenAIRE

Aline Mara dos Santos

2011-01-01

Resumo: A tirosino-quinase de adesão focal (FAK) tem papel crítico na mediação da migração, sobrevivência e proliferação celular. Estudos anteriores de nosso laboratório demonstraram que a FAK é ativada pelo estresse mecânico em miócitos cardíacos e que ela se coimunoprecipita com a miosina sarcomérica. No presente trabalho foi demonstrado que o domínio FERM da FAK medeia à interação com a miosina sarcomérica, sendo que esta interação leva a inibição da autofosforilação da FAK, enquanto que a...

Identificação dos citoplasmas "S", "T" e "N" via PCR em populações de cebola no Vale do São Francisco Identification of "S", "T" and "N" cytoplasm via PCR in onion populations in the Sao Francisco river Valley, Brazil

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Carlos Antonio F Santos

2008-09-01

Full Text Available A identificação do tipo de citoplasma em cebola foi facilitada com a publicação de primers de DNA específicos para os tipos "S", "T" e "N". O objetivo do presente trabalho foi identificar, por meio de marcadores moleculares, o tipo de citoplasma presente na cultivar de cebola BRS São Francisco e numa população experimental em desenvolvimento na Embrapa Semi-Árido, de forma a orientar o desenvolvimento de híbridos de cebola adaptados à região. Na amostra de 19 plantas da 'BRS Alfa São Francisco' observou-se a amplificação de fragmentos de 180 pb, ausência do fragmento de 414 pb com os primers 5'cob e a amplificação do fragmento de 473 pb dos primers OrfA501, indicando que o citoplasma presente nesta população é o citoplasma T. Foi também identificada a presença de citoplasma N na população BRS Alfa São Francisco. Na amostra de 44 plantas da população experimental cascuda-bronzeada observou-se a amplificação de produtos consistentes com os fragmentos de 180 e 414 pb dos primers 5'cob, bem como a amplificação de fragmento de 473 pb dos primers OrfA501, indicando que o citoplasma presente na população é o citoplasma S. Não foi identificada a presença de citoplasma N na população cascuda-bronzeada. Estes resultados indicam a possibilidade do desenvolvimento de híbrido tropical, tendo como base a cultivar BRS Alfa São Francisco, enquanto que, na população cascuda-bronzeada será necessário a introgressão do citoplasma "N" para o desenvolvimento de linhagens mantenedoras.The identification of the onion cytoplasm was obtained by the development of DNA specific primers to "S", "T" and "N" types. The goal of this work was the identification of the cytoplasm type in two different onion populations in the São Francisco river Valley, in order to facilitate the development of onion hybrids from Northeast Brazil. In the sample of 19 plants of the BRS Alfa São Francisco cultivar was observed the amplification of

Experiencia con las reacciones adversas asociadas con el interferón alfa 2b recombinante en hematología Experience with the adverse reactions associated with recombinant alpha 2b interferon in hematology

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Idalmis Brito Pascual

2005-08-01

Full Text Available El interferón alfa 2b es una citoquina con actividad antiviral, antiproliferativa e inmunomoduladora que ha demostrado ser eficaz para el tratamiento de enfermedades virales y neoplásicas. El presente trabajo se realizó con el propósito de describir la frecuencia de las reacciones adversas asociada con la administración de Heberon alfa R (interferón alfa 2b recombinante en enfermedades hematológicas. Se analizaron las características de base de los pacientes, así como la frecuencia, intensidad y relación de causalidad de los eventos reportados. Los eventos adversos más frecuentes fueron las manifestaciones del síndrome gripal que incluyó fiebre, escalofrío, astenia, mialgias, anorexia y cefalea. Estas manifestaciones son transitorias y responden al tratamiento con antiinflamatorios no esteroideos. La mayoría de los eventos fueron de intensidad leve a moderada y no requirieron la suspensión del tratamiento. En conclusión, el Heberon alfa R es un fármaco relativamente seguro para el tratamiento de trastornos hematológicosAlpha 2b interferon is a cytokine with antiviral, antiproliferative and immunomodulator activity that has proved to be efficient for the treatment of viral and neoplastic diseases, This paper is aimed at describing the frequency of adverse reactions associated with the administration of alpha R Heberon (recombinant alpha 2b interferon in hematological diseases. The basic characteristics of the patients, as well as the frequency, intensity and causality relation of the reported events were analyzed. The most common adverse effects were the manifestations of the gripal syndrome that included fever, chills, astenia, myalgia, anorexia and headache. These manifestations are transitory and respond to the treatment with non-steroideal antiinflammatory drugs. Most of the events were from mild to moderate intensity and did not require the suspension of the treatment. To conclude, alpha R Heberon is a relatively safe drug

Determination of the total cross section in proton-proton collisions at the LHC at √(s) = 8 TeV from elastic scattering using the ALFA sub-detector of ATLAS

Energy Technology Data Exchange (ETDEWEB)

Heinz, Christian; Dueren, Michael; Kreutzfeldt, Kristof; Stenzel, Hasko [JLU Giessen (Germany)

2015-07-01

The ALFA (Absolute Luminosity for ATLAS) Roman Pot detector system is part of the forward instrumentation of ATLAS located about 240 m away from the interaction point in the LHC tunnel. ALFA consists of a scintillating fibre tracker housed in vertical Roman Pots which enables the measurement of elastic proton-proton scattering at small scattering angles. In 2012 data were recorded at a centre-of-mass energy of √(s) = 8 TeV during a fill with special beam optics of the LHC with β* = 90 m and parallel-to-point focusing. The four-momentum transfer t is measured for elastically scattered protons and the differential elastic cross section is measured. In this talk a preliminary determination of the total cross section and of the slope of the elastic cross section at small vertical stroke t vertical stroke obtained from a fit to the differential cross section using the optical theorem is reported.

Cyclic changes in gene expression induced by Peg-interferon alfa-2b plus ribavirin in peripheral blood monocytes (PBMC of hepatitis C patients during the first 10 weeks of treatment

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Edenberg Howard J

2008-11-01

Full Text Available Abstract Background and Aims This study determined the kinetics of gene expression during the first 10 weeks of therapy with Pegylated-interferon-alfa2b (PegIntron™ and ribavirin (administered by weight in HCV patients and compared it with the recently completed Virahep C study 12 in which Peginterferon-alfa2a (Pegasys™ and ribavirin were administered. Methods RNA was isolated from peripheral blood monocytes (PBMC from twenty treatment-naïve patients just before treatment (day 1 and at days 3, 6, 10, 13, 27, 42 and 70 days after treatment. Gene expression at each time was measured using Affymetrix microarrays and compared to that of day 1. Results The expression of many genes differed significantly (p ≤ 0.001 and changed at least 1.5-fold at days 3 (290 probes and 10 (255 probes, but the number dropped at days 6 (165 and 13 (142. Most genes continued to be up regulated throughout the trial period. A second group of genes, including CXCL10, CMKLR1 (chemokine receptor 1, TRAIL, IL1Rα and genes associated with complement and lipid metabolism, was transiently induced early in treatment. CDKN1C (cyclin kinase inhibitor 1 was induced early but repressed at later times. Genes induced at later times were mostly related to blood chemistry and oxygen transport. By week 10, 11 of the patients demonstrated a positive response to therapy, and the final sustained viral response (SVR was 35%. The levels of gene induction or decrease was very similar to that previously reported with Pegasys/ribavirin treatment. Conclusion The response to Pegintron/ribavirin was similar to that reported for Pegasys/ribavirin despite some differences in the amount administered. We did not detect major differences at the genomic level between patients responding to treatment or non-responders, perhaps because of limited power. Gene induction occurred in a cyclic fashion, peaking right after administration of interferon and declining between administrations of the drug. Our

Growth and production of onion cultivars in organic system in a nontraditional planting periodCrescimento e produção de sete cultivares de cebola em sistema orgânico em plantio fora de época

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Marcelle Michelotti Bettoni

2013-10-01

Full Text Available This experiment was conducted in the organic area of Canguiri experimental farm, in the metropolitan area of Curitiba-PR, aiming to evaluate the growth of open pollinated onion cultivars, in organic system, seeded in January – a nontraditional period. The experimental design was completely randomized, with three replications and seven cultivars: Franciscana IPA-10 (purple, Vale Ouro IPA-11 e Brisa IPA-12 (from Empresa Pernambucana de Pesquisa Agropecuária – IPA, Alfa Tropical (from Embrapa Hortaliças, Alfa São Francisco (VIII cycle e Alfa São Francisco-RT (thrips resistant – genotype under test; from Embrapa Semi-árido e BR-29 (Topseed- Agristar. Biometric linear and nonlinear evaluations and productive characteristics were undertaken. We verified that the organic production of onion established in a nontradicional period in metropolitan area of Curitiba is possible, mainly for the following cultivars of Embrapa: Alfa São Francisco, Alfa Tropical and Alfa São Francisco-RT that showed the best performance and productivity. O presente trabalho foi conduzido na área experimental de olericultura orgânica da estação do Canguiri-UFPR, região metropolitana de Curitiba, com o objetivo de avaliar o crescimento de cultivares de cebola de polinização aberta, em sistema orgânico, com semeadura em janeiro, fora da época tradicional. O delineamento experimental foi inteiramente casualizado, com três repetições e sete cultivares: Franciscana IPA-10 (roxa, Vale Ouro IPA-11 e Brisa IPA-12, (Empresa Pernambucana de Pesquisa Agropecuária-IPA, Alfa Tropical (Embrapa Hortaliças, Alfa São Francisco (ciclo VIII e Alfa São Francisco-RT (resistente ao tripes- genótipo em teste da Embrapa Semi-árido e BR-29 (Topseed- Agristar. Foram realizadas avaliações biométricas lineares e não lineares e características produtivas. Verificou-se que a produção orgânica de cebola estabelecida fora do período tradicional da região metropolitana

Participação da via alfa-2 adrenérgica no controle da secreção do hormônio do crescimento no período pré-púbere em novilhas da raça Nelore: adrenergic in the control of growth hormone secretion in the pre-puberty period of the Nellore heifers

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Emiliana de Oliveira Santana Batista

2011-08-01

Full Text Available O objetivo deste trabalho foi investigar a variação na secreção de GH em resposta ao tratamento com clonidina, agonista alfa-2 adrenérgico, no período pré-púbere de novilhas da raça Nelore, e desta forma obter informações neuroendócrinas envolvidas no processo de maturação sexual destes animais. A administração de clonidina (10 µg/kg, I.V., amostras 15 min por 4h foi feita nas novilhas aos oito (n =4, 12(n = 5 e 15 meses de idade (n = 4. A concentração de GH foi quantificada por radioimunoensaio (sensibilidade = 0,25 ng/mL, CV = 16%. Aos oito meses, a administração do estimulador alfa-2 adrenérgico aumentou a concentração de GH, área total de picos, área total de secreção de GH e amplitude do maior pico e reduziu o tempo para aparecimento de pico (P < 0,05. A administração de clonidina aumentou a concentração de GH aos 15 meses, e aos 12 meses, em intervalos restritos (P < 0,05. O uso da clonidina estimulou a secreção de GH em novilhas Nelore pré-púberes. Este efeito foi mais evidente nas novilhas aos oito meses, comparado aos 12 e 15 meses de idade.

Eletrencefalograma quantitativo e topográfico (mapeamento cerebral: estudo do padrão normal para uma população adulta

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ANGHINAH RENATO

1998-01-01

Full Text Available Realizamos estudo com o intuito de normatizar a atividade elétrica cerebral de base de indivíduos adultos e normais, com EEG quantitativo e topográfico. Foram avaliados 20 pacientes que apresentaram exames clínico, laboratoriais e de análise visual do EEG normais, além do mini-exame do estado mental com escores superiores a 27 pontos. Após a aplicação da transformada rápida de Fourier (FFT encontramos distribuição do espectro com padrão monomodal no histograma de barras às custas de maiores potenciais em regiões posteriores na faixa de frequência alfa. Ao analisarmos a média dos espectros nas diferentes bandas de frequência (delta, teta, alfa, beta 1, beta 2, beta 3 encontramos alguns padrões que diferem dos achados tradicionalmente descritos na eletrencefalografia com análise visual, tais como a presença de atividade alfa em regiões anteriores e atividade delta difusa durante a vigília. Os comportamentos das bandas beta 2, e beta 3, também com distribuição difusa, não são os classicamente encontrados. Por outro lado, outros achados são congruentes à análise visual, como o predomínio alfa posterior e a maior presença de atividade teta em regiões centrais.

LHC Report: special run with de-squeezed beams for ATLAS/ALFA and TOTEM

CERN Multimedia

Helmut Burkhardt for the LHC team

2015-01-01

The main high-luminosity proton-proton run of the LHC is complemented by one week per year of special proton-proton runs. The special runs are performed with larger beam sizes at the interaction points to allow the forward physics experiments, TOTEM and ATLAS/ALFA, the chance to make precise measurements of protons as they emerge from collisions at small angles.   In standard high-luminosity operation, the beams are squeezed to give small beam sizes at the interaction points to maximise the collision rates. The “squeeze” takes place at top energy and the beam size at the centre of ATLAS (IP1) and CMS (IP5) is reduced from 66 micrometres at the top of the ramp to 18 micrometres before colliding beams are established. Protons that avoid the fate of an inelastic collision but yet still interact – in elastic or diffractive events – are scattered and emerge in the forward direction. The reduction in beam size has a side effect of increasing the an...

VizieR Online Data Catalog: UV counterparts in HI clouds using ALFA surveys (Donovan+, 2015)

Science.gov (United States)

Donovan Meyer, J.; Peek, J. E. G.; Putman, M.; Grcevich, J.

2017-10-01

GALFA-HI is a survey of Galactic HI conducted with the ALFA seven-beam feed array on the 305 m Arecibo antenna. The survey has both high spatial (FWHM~4') and velocity (0.18 km/s) resolution over 13000 (7520 in DR1) degrees2 of sky between -650 and 650 km/s. Details of the observations and data reduction can be found in Peek et al. (2011ApJS..194...20P). The ALFALFA HI-line survey, now 40% complete, also uses the Arecibo Observatory and its seven-beam feed array to detect potential dwarf galaxies in the vicinity of the Milky Way. The survey, which covers over 7000 (2800 in α.40) deg2 of sky out to 18000 km/s, has the sensitivity to detect 105 Mȯ clouds with 20 km/s linewidths at a distance of 1 Mpc. (2 data files).

Feocromocitoma: uma causa rara de hipertensão arterial na infância Pheochromocytoma: a rare cause of hypertension in childhood

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Flávia Carolina D. Georgetti

2007-09-01

Full Text Available OBJETIVO: Relatar o caso de um adolescente com feocromocitoma, uma causa rara de hipertensão arterial na infância. DESCRIÇÃO: Adolescente internado em unidade de terapia intensiva infantil em decorrência de emergência hipertensiva, conseqüente à presença de feocromocitoma em adrenal esquerda, diagnosticado por meio de tomografia computadorizada do abdome e pela dosagem de adrenalina e noradrenalina urinárias. O paciente foi submetido à adrenalectomia esquerda, após o uso de alfa-bloqueador para controle do quadro hipertensivo. O anatomopatológico confirmou o diagnóstico do tumor. No pós-operatório, o paciente permaneceu estável, possibilitando a suspensão dos anti-hipertensivos. COMENTÁRIOS: Os feocromocitomas são tumores capazes de produzir catecolaminas, especialmente adrenalina e/ou noradrenalina. Cerca de 85 a 95% dos tumores são únicos, benignos e encontrados na medula adrenal. O feocromocitoma é um tumor de incidência rara e apenas 10 a 20% ocorrem na infância, representando uma causa rara de hipertensão arterial. Esta última é um sinal freqüente na maioria das crianças (80%, podendo ser acompanhada por cefaléia e sudorese. A encefalopatia hipertensiva consiste em uma forma de apresentação excepcional da doença. O diagnóstico pode ser realizado, na maioria dos casos, pela tomografia de abdome e pela dosagem das catecolaminas e seus metabólitos produzidos pelo tumor. O tratamento de escolha consiste na ressecção completa do tumor após o preparo farmacológico do paciente com o uso de alfa-bloqueador. No pós-operatório, a maioria dos pacientes evolui com controle do quadro de hipertensão arterial.OBJECTIVE: Report an adolescent with pheochromocytoma, a rare cause of hypertension in childhood. CASE DESCRIPTION: Adolescent admitted to the pediatric intensive care unit due to hypertension, secondary to the presence of pheochromocytoma on the left adrenal. Diagnosis of the pheochromocytoma was made by

Determination of the total cross section in proton-proton collisions at the LHC at √(s) = 8 from elastic scattering using the ALFA sub-detector of ATLAS

Energy Technology Data Exchange (ETDEWEB)

Heinz, Christian; Stenzel, Hasko; Dueren, Michael [2. Physikalisches Institut, Universitaet Giessen (Germany)

2016-07-01

The ALFA (Absolute Luminosity for ATLAS) Roman Pot detector system is part of the forward instrumentation of ATLAS located about 240 m away from the interaction point in the LHC tunnel in both directions. ALFA consists of a scintillating fibre tracker housed in vertical Roman Pots which enables the measurement of elastic proton-proton scattering at small scattering angles. In 2012 data were recorded at a centre-of-mass energy of √(s) = 8 TeV during a fill with special beam optics of the LHC with β* = 90 m and parallel-to-point focusing. The four-momentum transfer t is measured for elastically scattered protons and used to extract the differential elastic cross section. In this talk a preliminary determination of the total cross section and of the slope of the elastic cross section at small vertical stroke t vertical stroke obtained from a fit to the differential cross section using the optical theorem is reported. In addition a second run at √(s) = 8 TeV with a special beam optics of β* = 1 km, providing access to the Coulomb-nuclear interference region, is being analysed. Preliminary analysis results from this run are presented as well.

Tempo de protrombina e de tromboplastina parcial ativada em caprinos criados extensivamente no Estado do Rio Grande do Norte Prothrombin time and activated partial thromboplastin time in goats raised extensively at Rio Grande do Norte State, Brazil

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Benito Soto-Blanco

2009-03-01

Full Text Available Neste trabalho objetivou-se estabelecer os padrões de normalidade para tempo de protrombina (TP e tempo de tromboplastina parcial ativada (TTPA em caprinos criados extensivamente no estado do Rio Grande do Norte. Foram utilizados 40 caprinos SRD, 13 machos e 27 fêmeas, adultos, com idades entre 1 e 6 anos, todos clinicamente sadios. As amostras sanguíneas foram coletadas por venopunção jugular em tubos contendo citrato de sódio a 3,8%. O plasma foi imediatamente separado por centrifugação e refrigerado a ± 4ºC e, posteriormente, submetido às determinações de TP e TTPA por meio de métodos manuais utilizando kits comerciais. Os valores obtidos para caprinos da região, de 25,4 ± 0,90 e 39,5 ± 1,41 segundos para TP e TTPA, respectivamente, estavam dentro da normalidade.This study aimed to establish normal pattern for the prothrombin time (PT and activated partial thromboplastin time (APTT in goats extensively raised at Rio Grande do Norte state, Brazil. A total of 40 clinically healthy adult goats (13 males and 27 females aged 1 to 6 years-old was used. Blood samples were collected from jugular vein in tubes containing 3.8% of sodium citrate. The plasma was immediately separated by centrifugation and refrigerated at ± 4ºC and after submitted to PT e APTT determinations through manual methods using commercial kits. The results for goats in the region of 25.4 ± 0.90 and 39.5 ± 1.41 seconds, respectively, for PT and APTT were in an adequate range.

Avanços no diagnóstico e tratamento da sepse Advances in sepsis diagnosis and treatment

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Paulo R.A. Carvalho

2003-11-01

Full Text Available OBJETIVO: Apresentar uma revisão crítica e atualizada sobre a sepse, principalmente os aspectos diagnósticos e terapêuticos. FONTES DOS DADOS: Pesquisa bibliográfica em periódicos indexados em base Medline, tanto de revisão como ensaios clínicos e pesquisa laboratorial. SÍNTESE DOS DADOS: A Conferência Internacional sobre Definição de Sepse ampliou a relação de possíveis sinais clínicos e laboratoriais de sepse, o que poderá permitir a suspeição e manejo iniciais mais eficazes. Na avaliação laboratorial, além da pesquisa do agente infectante, vários marcadores da resposta inflamatória tais como as citoquinas inflamatórias e a procalcitonina, têm sido identificados, mas ainda sem sensibilidade e especificidade suficientes para diagnóstico seguro. Quanto ao tratamento, as intervenções precoces sobre os distúrbios hemodinâmicos continuam sendo primordiais para o desfecho, assim como o uso racional de antimicrobianos. Terapias de remoção de toxinas e de aumento da resposta imune inata ainda não provaram definitivamente seu valor. O uso de bloqueadores da resposta inflamatória isolados, em qualquer fase do seu estágio, falhou em reduzir a mortalidade. O corticóide ressurge com resultados animadores, mesmo em pacientes sem insuficiência adrenal relacionada à sepse. A proteína C ativada (drotrecogina-alfa, em um grande estudo, mostrou redução de 6% de mortalidade em uma amostragem selecionada, oferecendo uma possibilidade de melhor prognóstico na sepse. CONCLUSÕES: Comparativamente aos avanços dos últimos anos, pouco se obteve com relação à diminuição de mortalidade por sepse, pela complexidade das relações patógeno-hospedeiro. A regulação individual de cada reação do hospedeiro não mostrou o efeito esperado. Algumas estratégias, já conhecidas, foram reafirmadas como benéficas, e outras, como o uso de corticóide e a proteína C ativada, estão surgindo como terapias promissoras. As

Characterization of autoantibodies in autoimmune hemolytic anemia following treatment with interferon alfa

International Nuclear Information System (INIS)

Bencomo Hernandez, Antonio; Gutierrez Diaz, Adys; Avila Cabrera, Onel; Rodriguez, Luis Ramon

2012-01-01

We studied 13 patients with chronic myeloid leukemia and autoimmune hemolytic anemia induced by interferon alfa. They underwent tests for immune protein detection and characterization of IgG subclasses in RBCs by direct antiglobulin test (PAD) and the microplate technique. Also they were applied ELISA test for quantifying immunoglobulins in the red blood cells. It was detected the presence of IgG and C3 in 53.84 % of cases, IgG alone in 23.07 % and in 15.38 % were identified IgG and IgA autoantibodies. In 11 patients the presence of IgG1 was showed and also in one case the subclass IgG3 autoantibodies was identified. The ELISA detected antibodies at concentrations of 183 IgG molecules per erythrocyte in a patient with negative PAD. In high-grade hemolysis patients, it was found a concentration of autoantibodies between 1 500 and 3 180 molecules of IgG per erythrocyte, while in low-grade hemolysis patients it behaved between 183 and 1 000 molecules. There was a negative correlation between Hb and plasma haptoglobin values with the number of IgG molecules per erythrocyte and a positive correlation between the latter with the reticulocyte count

Purificación y caracterización de alfa-amilasa de penicillium commune producida mediante fermentación en fase sólida

OpenAIRE

Espinel, Esperanza; López, Elizabeth

2010-01-01

Este estudio reporta la purificación y caracterización parcial de una alfa-amilasa producida por Penicillium commune mediante fermentación en fase sólida, empleandoyuca blanca colombiana (Manihot esculenta Crantz) como soporte. La enzima fue purificada por precipitación fraccionada con sulfato de amonio, cromatografía de intercambio aniónico (DEAE-Sephadex A-50), cromatografía de filtración por gel (Sephadex G-75) y cromatografía de intercambio catiónico (CM-Sephadex C-50) obteniendo una acti...

Seguridad de la terapia de interferón alfa 2b recombinante más ribavirina en la hepatitis crónica C

OpenAIRE

Sánchez Rodríguez, Yoan Antonio; Arús Soler, Enrique; López Saura, Pedro; Nodarse Cuní, Hugo

2011-01-01

INTRODUCCIÓN: la hepatitis crónica C ha adquirido rango de pandemia. El virus de la hepatitis C se ha convertido en la causa principal de hepatitis crónica, cirrosis hepática, hepatocarcinoma, y trasplante de hígado a nivel mundial. OBJETIVO: identificar los efectos adversos asociados a la terapia combinada interferón alfa 2b recombinante más ribavirina durante la evolución del tratamiento y hasta 8 semanas después de finalizado, así como los principales efectos asociados a salidas temporales...

Dose inseminante utilizada na fertilização artificial de ovócito de piracanjuba (Brycon orbignyanus

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Viviane de Oliveira Felizardo

2010-10-01

Full Text Available A piracanjuba (Brycon orbignyanus Valenciennes, 1849 é uma espécie de peixe migratória, ameaçada de extinção. O objetivo do presente estudo foi determinar a dose inseminante na fertilização artificial de ovócitos de piracanjuba. Para isso, utilizou-se delineamento em blocos casualizados, com quatro tratamentos e três repetições. Três casais de piracanjuba, selecionados dos tanques de reprodutores da Estação Ambiental de Itutinga (EAI - CEMIG, no período de piracema 2006/2007, receberam aplicação de hormônio extrato bruto de hipófise de carpa (EBHC para obtenção dos gametas. Adotaram-se quatro tratamentos diferentes para a fertilização de 0,1 grama de ovócitos: 10µL, 20µL, 30µL e 40µL de sêmen. As amostras foram ativadas com 5 mL de água do próprio tanque e, em seguida, levadas para incubadoras, dotadas de renovação constante de água, à temperatura de 28ºC. Após 8 e 16 horas, analisaram-se as taxas de fertilização (ovos viáveis e de eclosão dos ovos, respectivamente. Os resultados obtidos foram comparados pelo teste de Tukey a 5%. As relações sêmen-ovócitos testadas não alteraram as taxas de fertilização e eclosão (P>0,05. O número de espermatozoides-ovócitos, variando de 10,4 x10(5 a 41,6 x10(5, foi eficiente para obtenção de boas taxas de fertilidade.

Comportamiento de glicinina, beta-conglicinina y alfa-amilasa en semillas de soja deterioradas y no deterioradas Glycinin, beta-conglycinin and alpha-amylase behaviour in artificially deteriorated and not deteriorated soybean

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Adriana Rita Salinas

2002-08-01

Full Text Available El objetivo del trabajo fue estudiar el comportamiento de glicinina y beta-conglicinina y la actividad de alfa-amilasa en semillas deterioradas y no deterioradas de 10 cultivares de soja [Glycine max (L. Merr.]. Las semillas se sometieron a dos tratamientos: deterioradas por envejecimiento acelerado y no deterioradas. Se determinó la presencia de las proteínas de reserva a partir de semillas con 0, 3 y 8 días de germinadas por electroforesis en geles de poliacrilamida (SDS-PAGE. La actividad de la alfa-amilasa se determinó bioquímicamente en semillas con 0, 3, 8 y 12 días de germinadas. No hubo diferencias en la presencia de bandas de glicinina y beta-conglicinina en semillas no deterioradas hasta los 8 días de germinadas. Las semillas deterioradas se comportaron en forma similar a las no deterioradas. La actividad de la alfa-amilasa aumentó en semillas germinadas hasta 8 días y disminuyó a los 12 días. En semillas deterioradas la actividad enzimática disminuyó con respecto a las no deterioradas. El deterioro artificial no afectó la presencia de glicinina y beta-conglicinina pero alteró la actividad de la alfa-amilasa hasta los 12 días de germinación. Los cultivares estudiados mostraron comportamiento diferencial frente a la actividad de esta enzima.The objective of this research was to study the behaviour of the storage proteins glycinin and beta-conglycinin and the alpha-amylase activity in artificially deteriorated and not deteriorated soybean [Glycine max (L. Merr.] seeds of 10 cultivars. The seeds were submitted to two treatments: deteriorated by accelerated aging and not deteriorated seeds. The presence of glycinin and beta-conglycinin were determined in seeds with 0, 3 and 8 days of germination using polyacrylamid gel electrophoresis (SDS-PAGE. The alpha-amylase activity was biochemically determined in seeds with 0, 3, 8 and 12 days of germination. There were no differences in the presence of glycinin and beta

Avaliação do perfil hematológico de portadores de talassemia alfa provenientes das regiões Sudeste e Nordeste do Brasil Evaluation of alpha thalassemia carriers hematologic profile from Southeast and Northeast Brazilian regions

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Gislane L. V. Oliveira

2006-06-01

Full Text Available A talassemia alfa é uma anemia hereditária resultante da síntese deficiente de cadeias alfa, provocando um excesso relativo de cadeias beta, que vão formar tetrâmeros identificados como hemoglobina H (Hb H no indivíduo adulto. Para direcionar o diagnóstico laboratorial desta anemia, a análise dos índices eritrocitários, a eletroforese em acetato de celulose em pH neutro e a pesquisa de corpos de inclusão de Hb H são essenciais. O objetivo deste estudo foi traçar o perfil hematológico, por meio dos índices eritrocitários, dos portadores de talassemia alfa das regiões Sudeste e Nordeste do Brasil. Foram analisadas 1.010 amostras de sangue periférico após consentimento informado. Os índices eritrocitários como contagem de glóbulos vermelhos (RBC, dosagem de hemoglobina (HGB, hematócrito (HCT, volume corpuscular médio (VCM, hemoglobina corpuscular média (HCM e concentração de hemoglobina corpuscular média (CHCM foram fornecidos por aparelhos automatizados com controle de qualidade interno e externo. Para o diagnóstico de talassemia alfa foram utilizados testes de triagem e complementares para talassemias, como eletroforese em pH neutro e pesquisa de corpos de inclusão de Hb H com coloração de azul cresil brilhante. Comparando-se os valores hematológicos observados nos dois grupos, notou-se que, em ambas as regiões, os índices com valores discrepantes foram os níveis de HGB e HCT, sendo a maior freqüência de variação observada entre as mulheres. Nos portadores do fenótipo alfa talassêmico da região Nordeste, todos os índices eritrocitários estavam abaixo dos valores de normalidade. Estes resultados evidenciam a necessidade de melhor avaliação do perfil hematológico de talassemia alfa em diferentes regiões, considerando-se os interferentes ambientais para um diagnóstico mais preciso.Alpha thalassemia is a hereditary anaemia resulting from deficient synthesis of the alpha chains, causing a relative

Multicenter, noninterventional, post-marketing surveillance study to evaluate dosing of recombinant human follicle-stimulating hormone using the redesigned follitropin alfa pen in women undergoing ovulation induction

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Nawroth F

2015-04-01

Full Text Available Frank Nawroth,1 Andreas Tandler-Schneider,2 Wilma Bilger3 1Centre for Reproductive and Prenatal Medicine, Endocrinology and Osteology, Hamburg, Germany; 2Center for Reproductive Medicine, Fertility Center Berlin, Berlin, Germany; 3Medical Affairs, Fertility, Endocrinology and General Medicine, Merck Serono GmbH, Darmstadt, Germany (an affiliate of Merck KGaA, Darmstadt, Germany Abstract: This prospective, noninterventional, post-marketing surveillance study evaluated doses of recombinant human follicle-stimulating hormone (r-hFSH using the redesigned follitropin alfa pen in women who were anovulatory or oligomenorrheic and undergoing ovulation induction (OI alone or OI with intrauterine insemination. The primary endpoint was the proportion of patients who achieved monofollicular or bifollicular development (defined as one or two follicles 15 mm. Secondary endpoints included characteristics of ovulation stimulation treatment, such as mean total and mean daily r-hFSH doses. Data were analyzed for 3,193 patients from 30 German fertility centers. The proportion of patients with monofollicular or bifollicular development was 71.1% (n=2,270 of a total of 3,193 patients; intent-to-treat population. The mean±standard deviation total and daily doses of r-hFSH were 696.9±542.5 IU and 61.7±29.4 IU, respectively. The three doses prescribed most frequently were: 37.5 IU (n=703 from N=3,189; 22.0%, 50.0 IU (n=1,056 from N=3,189; 33.1%, and 75.0 IU (n=738 from N=3,189; 23.1% on the first day of stimulation; and 37.5 IU (n=465 from N=3,189; 14.6%, 50.0 IU (n=922 from N=3,189; 28.9%, and 75.0 IU (n=895 from N=3,189; 28.1% on the last day of stimulation. This noninterventional, post-marketing surveillance study found that monofollicular or bifollicular development was achieved in 71% of patients studied and the small dose increment (12.5 IU of the redesigned follitropin alfa pen allowed individualized treatment of women undergoing OI. Keywords: ovulation

Resposta ao estresse: II. Resiliência e vulnerabilidade

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Nicole Leite Galvão-Coelho

2015-06-01

Full Text Available ResumoA crescente exposição a estressores na vida cotidiana aumentou significativamente a investigação da resposta ao estresse nas duas últimas décadas. Embora associada a consequências negativas, pois muitas patologias físicas e mentais são desencadeadas por exposição crônica a estressores, esta resposta é indispensável para sobrevivencia do indivíduo e é extremamente adaptativa quando ativada de forma aguda. Na parte I desta revisão foram abordados os conceitos de homestase e alostase e os sistemas fisiológicos ativados durante a resposta ao estresse. Na parte II serão discutidos fatores que modulam a resposta ao estresse tais como sexo, temperamento, períodos críticos do desenvolvimento e a presença ou ausência de suporte social. A interação entre os fatores genéticos e ambientais gera os perfis da resposta psicofisiológica que caracterizam os fenótipos de susceptibilidade e resiliência frente aos estressores e sua relação com uma patologia mental cada vez mais presente na sociedade moderna, o transtorno de estresse pós-traumático.

Acute pancreatitis attributed to the use of interferon alfa-2b.

Science.gov (United States)

Eland, I A; Rasch, M C; Sturkenboom, M J; Bekkering, F C; Brouwer, J T; Delwaide, J; Belaiche, J; Houbiers, G; Stricker, B H

2000-07-01

Two patients experienced episodes of acute pancreatitis shortly after starting treatment with interferon alfa-2b (IFN-alpha) for a chronic hepatitis C infection. The first patient was a 40-year-old man who developed acute pancreatitis after 15 weeks of treatment with 3 MU IFN-alpha subcutaneously (SC) 3 times weekly and 1200 mg ribavirin. After disappearance of symptoms and normalization of laboratory values, oral intake of solid foods and IFN-alpha therapy were restarted. Within hours, a relapse of acute pancreatitis occurred. A rechallenge with IFN-alpha 4 days later was followed by a prompt increase in serum lipase level, and IFN-alpha therapy was discontinued. The second patient was a 38-year-old man who developed acute pancreatitis 2 hours after SC administration of 5 MU IFN-alpha. Ultrasound endoscopy showed sludge in the gallbladder. The patient was rechallenged 5 weeks later with 3 MU IFN-alpha SC. Although serum amylase and lipase levels increased after readministration of IFN-alpha, treatment was continued. The patient was readmitted 2 weeks later with severe abdominal pain, and IFN-alpha administration was discontinued. Considering the temporal relationship between the start of IFN-alpha treatment and development of acute pancreatitis, the absence of other clear etiologic factors for acute pancreatitis, disappearance of symptoms after discontinuation of IFN-alpha, and positive reactions to rechallenge, IFN-alpha is the most probable cause for development of acute pancreatitis in these patients.

Study of forward elastic pp scattering at √ s = 8 TeV with the ALFA detector

DEFF Research Database (Denmark)

Stark, Simon Holm

The elastic proton-proton scattering data analyzed in this thesis was taken at the LHC with the ALFA detector at sqrt(s) = 8 TeV with a beta* collision optics which gave access to the Coulomb-Nuclear-Interference region. An improved procedure was used to fit the observed number of elastic events......_tot, and the ratio of the real to imaginary forward elastic scattering amplitude, rho, are: sigma_tot = 99.1 +/- 0.3 (stat.) +/- 1.6 (syst.) mb rho = 0.166 +/- 0.017 (stat.) +/- 0.019 (syst.) which are in agreement with previous measurements. As part of the analysis, the detector distance to the beam was determined...... with an improved method. A new background estimation procedure was developed and also implemented in the determination of the elastic track reconstruction efficiency. The experience gained in this thesis was used to optimize the experimental settings for a new data taking at sqrt(s) = 13 TeV with beta* = 2.5 km...

Long-term outcome of hepatitis B e antigen-positive patients with compensated cirrhosis treated with interferon alfa. European Concerted Action on Viral Hepatitis (EUROHEP)

DEFF Research Database (Denmark)

Fattovich, G; Giustina, G; Realdi, G

1997-01-01

The aim of this study was to evaluate whether interferon alfa (IFN-alpha) treatment-associated virological and biochemical remission improves survival in a cohort of 90 white patients with compensated cirrhosis caused by hepatitis B (Child A) followed for a mean period of 7 years. Inclusion...... criteria were biopsy-proven cirrhosis, hepatitis B e antigen (HBeAg) positivity, abnormal serum aminotransferase levels, exclusion of hepatitis delta virus, and absence of complications of cirrhosis. Of the 40 IFN-treated patients, 27 (67%) showed sustained HBeAg loss with alanine aminotransferase (ALT...

Hypofractionation for radiotherapy of prostate cancer using a low alfa/beta ratio - possible reasons for concerns? An example of five dimensional radiotherapy

International Nuclear Information System (INIS)

Lennernaes, Bo; Nilsson, Sten; Levitt, Seymour

2011-01-01

It is very attractive, due to the assumed low alfa/beta ratio of prostate cancer (PC), to construct new treatment schedules for prostate cancer using only a few large fractions of radiation (hypofractionation). This will widen the therapeutic window since the ratio for PC might be lower than that of the organs at risk (OAR). PC is an extremely variable disease and often contains both highly and poorly differentiated cells. It is reasonable to assume that different cells have different patterns of radiosensitivity, i.e. alfa/beta ratios and proliferation. In this study we will simulate the effect on the outcome of the treatment with different fractionations and different ratios. Material and methods. In this simulation we use an extension of the Linear Quadratic (LQ)/Biological Effective Dose (BED) formula called the dose volume inhomogeneity corrected BED (DVIC-BED). In the formula the tumour volume is divided in 50 subvolumes (step of 2%) and it is possible to calculate the relative effect of the treatment with different ratios (1.5, 4 and 6.5) in different subvolumes. Results. The simulations demonstrate that only a small portion (5-10%) of cells with a higher ratio will dramatically change the effect of the treatment. Increasing the total dose can compensate this, but this will on the other hand increase the dose to the OAR and also the risk for severe side effects. Conclusion. These simulations highlight possible reasons for concerns about the use of hypofractionation for pathologically heterogeneous tumours, such as prostate cancer, and also demonstrate the need for testing new treatment schedules using both high and low ratios

FibroTest is an independent predictor of virologic response in chronic hepatitis C patients retreated with pegylated interferon alfa-2b and ribavirin in the EPIC³ program.

Science.gov (United States)

Poynard, Thierry; Munteanu, Mona; Colombo, Massimo; Bruix, Jordi; Schiff, Eugene; Terg, Ruben; Flamm, Steven; Moreno-Otero, Ricardo; Carrilho, Flair; Schmidt, Warren; Berg, Thomas; McGarrity, Thomas; Heathcote, E Jenny; Gonçales, Fernando; Diago, Moises; Craxi, Antonio; Silva, Marcelo; Boparai, Navdeep; Griffel, Louis; Burroughs, Margaret; Brass, Clifford; Albrecht, Janice

2011-02-01

EPIC-3 is a prospective, international study that has demonstrated the efficacy of PEG-IFN alfa-2b plus weight-based ribavirin in patients with chronic hepatitis C and significant fibrosis who previously failed any interferon-alfa/ribavirin therapy. The aim of the present study was to assess FibroTest (FT), a validated non-invasive marker of fibrosis in treatment-naive patients, as a possible alternative to biopsy as the baseline predictor of subsequent early virologic (EVR) and sustained virologic response (SVR) in previously treated patients. Of 2312 patients enrolled, 1459 had an available baseline FT, biopsy, and complete data. Uni- (UV) and multi-variable (MV) analyses were performed using FT and biopsy. Baseline characteristics were similar as in the overall population; METAVIR stage: 28% F2, 29% F3, and 43% F4, previous relapsers 29%, previous PEG-IFN regimen 41%, high baseline viral load (BVL) 64%. 506 patients (35%) had undetectable HCV-RNA at TW12 (TW12neg), with 58% achieving SVR. The accuracy of FT was similar to that in naive patients: AUROC curve for the diagnosis of F4 vs F2=0.80 (p<0.00001). Five baseline factors were associated (p<0.001) with SVR in UV and MV analyses (odds ratio: UV/MV): fibrosis stage estimated using FT (4.5/5.9) or biopsy (1.5/1.6), genotype 2/3 (4.5/5.1), BVL (1.5/1.3), prior relapse (1.6/1.6), previous treatment with non-PEG-IFN (2.6/2.0). These same factors were associated (p ≤ 0.001) with EVR. Among patients TW12neg, two independent factors remained highly predictive of SVR by MV analysis (p ≤ 0.001): genotype 2/3 (odds ratio=2.9), fibrosis estimated with FT (4.3) or by biopsy (1.5). FibroTest at baseline is a possible non-invasive alternative to biopsy for the prediction of EVR at 12 weeks and SVR, in patients with previous failures and advanced fibrosis, retreated with PEG-IFN alfa-2b and ribavirin. Copyright © 2010 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.

Recursos tecnológicos e informacionais ativos na Encontros Bibli

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Araci Isaltina de Andrade Hillesheim

2010-10-01

Full Text Available As tecnologias da informação e comunicação permeiam o nosso processo de editoração científica. Entre os dias 14 e 16 de julho de 2010, realizou-se, em Florianópolis, no Auditório da Reitoria da Universidade Federal de Santa Catarina, o I Encontro Nacional de Usuários do SEER (a programação, vídeos e relatos estão disponíveis no site http://euseer.ibict.br/index.php/euseer1/euseer1. Foi uma satisfação receber e encontrar colegas editores, avaliadores, autores, bolsistas, leitores e principalmente nossos mestres no uso da Plataforma Open Journal Systems (http://pkp.sfu.ca/?q=ojs – ou melhor, toda a equipe do IBICT responsável pelos estudos, customização, desenvolvimento e disseminação da edição em português, resultando na adoção desse Sistema Eletrônico de Editoração de Revistas – SEER, utilizado por mais de 800 revistas científicas somente no Brasil (conforme registrado no Portal SEER - http://seer.ibict.br/.Acompanhar a evolução das ferramentas tecnológicas, aplicar os recursos de novas versões e a troca de conhecimentos tornam-se uma experiência enriquecedora nos processos de aprender-saber-fazer. Em julho desse ano, a administração do Portal Periódicos UFSC (http://www.periodicos.ufsc.br/ migrou a plataforma para a nova versão e novas funcionalidades foram ativadas pelos editores gerentes das revistas.A Encontros Bibli ativou algumas ferramentas, entre as quais, disponibilizar os acessos estatísticos dos arquivos publicados dos autores. Isto é, o autor que tem um artigo publicado em nossa revista poderá consultar, no seu perfil, os dados referentes aos respectivos acessos de cada artigo. Paralelamente, com a nova versão, houve mudanças no autoarquivamento, isto é, quando o autor submete um arquivo na plataforma, pode preencher os metadados com maior facilidade, pois estão disponibilizadas as abas nos idiomas português e inglês. Gradativamente, os metadados ganham maior destaque no tratamento

HIDROLISADO DE FÉCULA DE MANDIOCA COMO ADJUNTO DE MALTE NA FABRICAÇÃO DE CERVEJA: AVALIAÇÃO QUÍMICA E SENSORIAL

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Waldemar G. VENTURINI FILHO

1998-05-01

Full Text Available Em virtude da progressiva substituição dos adjuntos amiláceos pelos xaropes com alta concentração de maltose nas cervejarias brasileiras, o presente trabalho teve por objetivo comparar hidrolisados de milho e de mandioca, como adjunto de malte, na fabricação de cerveja tipo Pilsen, em escala de laboratório. Os hidrolisados foram produzidos a partir de amido de milho e fécula de mandioca, sendo que na liqüefação e sacarificação da fração amilácea destes produtos foram utilizadas, respectivamente, as enzimas comerciais Termamil (alfa amilase bacteriana e Fungamil (alfa amilase fúngica. Na fabricação das cervejas, a proporção de malte e hidrolisado foi de 2 para 1, na base do extrato. O mosto foi produzido pelo processo de infusão e após resfriamento e clarificação foi inoculado com levedura cervejeira de baixa fermentação. A fermentação transcorreu a 10 °C até 90% de atenuação do extrato aparente fermentável. As cervejas foram engarrafadas e, em seguida, maturadas a 0 °C, por 14 dias. Terminado o processo de fabricação, as cervejas foram analisadas química e sensorialmente. A semelhança na composição química dos hidrolisados de milho e de mandioca refletiu na composição química dos mostos e das cervejas. Não houve diferença estatística entre os mostos e entre as cervejas testadas para todos os parâmetros químicos analisados. Também, não existiu diferença sensorial entre as cervejas produzidas com hidrolisado de milho e hidrolisado de mandioca. Concluiu-se que a fécula de mandioca apresenta potencial de uso como matéria prima para a fabricação de xarope de maltose de uso cervejeiro e que há elevada probabilidade de sucesso no uso desse xarope para a fabricação de cervejas.The brazilians breweries replace progressively starchy adjuncts by sugary liquid adjuncts made with maize. Thus, the objective of present work was saccharify cassava and maize and use them as malt adjunct on Pilsen beer

Multifunctional Material Systems for Reconfigurable Antennas in Superconfigurable Structures

Science.gov (United States)

2016-01-05

glycol (as surfactant) and sodium hydroxide (NaOH) as the base; all chemicals were purchased from Alfa Company. Specific amounts and steps are shown in...tracking of 2µm gold nanorods in parallel electrode geometry. (middle right) video microscopy images of equilibrated colloids within electrode gap

Czynnik martwicy nowotworów alfa (TNF-α jako potencjalny osoczowy marker przebiegu stwardnienia rozsianego – badania wstępne

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Magdalena Justyna Kacperska

2014-07-01

Full Text Available Stwardnienie rozsiane (łac. sclerosis multiplex, SM zaliczane jest do częstych przewlekłych chorób ośrodkowego układu nerwowego wiążących się z postępującą niepełnosprawnością. Dotyczy zwykle osób pomiędzy 20. a 40.rokiem życia, przy czym dwukrotnie częściej chorują kobiety. Mimo wieloletnich badań nadal bardzo mało wiemy na temat patogenezy tej choroby. Klinicznie można wyróżnić cztery jej postaci, do najczęściej występujących należą postać rzutowo-remisyjna, wtórnie postępująca oraz pierwotnie postępująca. Patogeneza SM jest procesem bardzo skomplikowanym, biorą w nim udział m.in.: uszkodzenie bariery krew–mózg oraz bariery krew – płyn mózgowo-rdzeniowy, wtórny przerost astrogleju, toczący się proces zapalny i szeroko pojęta neurodegeneracja. Szczególną rolę w rozwoju SM, głównie na jego wczesnym etapie, odgrywa proces zapalny, w którym biorą udział różne subpopulacje komórek zapalnych. Dotychczasowe badania wskazują na szczególny udział w tym procesie czynnika martwicy nowotworów alfa (TNF-α zaliczanego do cytokin prozapalnych. Cel badania: Celem niniejszej pracy była analiza stężenia TNF-α w osoczu pacjentów z SM w rzucie i remisji choroby oraz jego korelacja z parametrami klinicznymi przebiegu choroby w celu oceny roli TNF-α jako potencjalnego markera zaawansowania SM. Poszukując najważniejszych producentów TNF-α we krwi chorych z SM, analizowano również związek pomiędzy stężeniem TNF-α w osoczu i liczebnością leukocytów we krwi oraz ich subpopulacji. Materiał i metodyka: W badaniu wzięło udział 37 pacjentów z grup badanych (20 w grupie z rzutem i 17 w grupie z remisją choroby oraz 30 osób z grupy kontrolnej. Od pacjentów pobrano do odpowiednich probówek krew żylną w ilości 4 ml. Wirowano ją (5000 rpm, 20 min, 23ºC, a następnie ostrożnie usuwano górną warstwę z osoczem, zabezpieczano i przechowywano w temperaturze –80ºC do czasu

Moldagem em prótese fixa: confecção do casquete de moldagem

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Aloísio Oro Spazzin

2017-12-01

Full Text Available O objetivo do artigo foi descrever a técnica de confecção de uma moldeira individual para moldagem de preparo total para coroa, também conhecida como casquete de moldagem, além de discutir fatores e princípios envolvidos no tema. Na presente técnica, é obtido um modelo de gesso da região do preparo e um alívio em cera é realizado em suas paredes axiais. Após a aplicação de um isolante, o casquete é confeccionado utilizando pó e líquido de resina acrílica ativada quimicamente indicada para confecção de restaurações provisórias. A resina acrílica é aplicada na região cervical utilizando um pincel, e em seguida, o restante do preparo é preenchido. Após finalizado o corpo do casquete, a área de retenção é criada (chapéu do casquete, a face vestibular é demarcada, e realizado acabamento e polimento.

Optimum Mix for Pervious Geopolymer Concrete (GEOCRETE Based on Water Permeability and Compressive Strength

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Abdulsalam Arafa Salaheddin

2017-01-01

Full Text Available The production of ordinary Portland cement (OPC consumes considerable natural resources and energy, and it also affects the emission of a significant quantity of CO2 in the atmosphere. This pervious geopolymer concrete study aims to explore an alternative binder without OPC. Pervious geopolymer concretes were prepared from fly ash (FA, sodium silicate (NaSiO3, sodium hydroxide (NaOH solution, and coarse aggregate (CA. The effects of pervious geopolymer concrete parameters that affect water permeability and compressive strength are evaluated. The FA to CA ratios of 1:6, 1:7,1:8, and 1:9 by weight, CA sizes of 5–10, 10–14, and 14–20 mm, constant NaSiO3/NaOH ratio of 2.5, alkaline liquid to fly ash (AL/FA ratios of 0.4, 0.5, and 0.6, and NaOH concentrations of 8, 10, and 12 M were the pervious geopolymer concrete mix proportions. The curing temperature of 80 °C for 24 h was used. The results showed that a pervious geopolymer concrete with CA of 10 mm achieved water permeability of 2.3 cm/s and compressive strength of 20 MPa with AL/FA ratio of 0.5, NaOH concentration of 10 M, and FA:CA of 1:7. GEOCRETE is indicated to have better engineering properties than does pervious concrete that is made of ordinary Portland cement.

Use of an integrated modelling and simulation approach to develop a simplified peginterferon alfa-2a dosing regimen for children with hepatitis C.

Science.gov (United States)

Brennan, Barbara J; Lemenuel-Diot, Annabelle; Snoeck, Eric; McKenna, Michael; Solsky, Jonathan; Wat, Cynthia; Mallalieu, Navita L

2016-04-01

The aim of the study was to simplify the dosing regimen of peginterferon alfa-2a in paediatric patients with chronic hepatitis C. A population pharmacokinetic (PK) model was developed using PK data from 14 children aged 2-8 years and 402 adults. Simulations were produced to identify a simplified dosing regimen that would provide exposures similar to those observed in the paediatric clinical trials and in the range known to be safe/efficacious in adults. Model predictions were evaluated against observed adult and paediatric data to reinforce confidence of the proposed dosing regimen. The final model was a two compartment model with a zero order resorption process. Covariates included a linear influence of body surface area (BSA) on apparent oral clearance (CL/F) and a linear influence of body weight on apparent volume of distribution of the central compartment (V1 /F). A simplified dosing regimen was developed which is expected to provide exposures in children aged ≥5 years similar to the dosing formula used in the paediatric clinical trial and within the range that is safe/efficacious in adults. This simplified regimen is approved in the EU and in other countries for the treatment of chronic hepatitis C in treatment-naive children/adolescents aged ≥5 years in combination with ribavirin. Pre-existing adult PK data were combined with relatively limited paediatric PK data to develop a PK model able to predict exposure in both populations adequately. This provided increased confidence in characterizing PK in children and helped in the development of a simplified dosing regimen of peginterferon alfa-2a in paediatric patients. © 2015 The British Pharmacological Society.

Interferentes eritrocitários e ambientais na anemia falciforme

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Naoum Paulo C.

2000-01-01

Full Text Available A anemia falciforme tem um desenvolvimento clínico extremamente variável que se caracteriza principalmente por diferentes graus de intensidade da anemia hemolítica. As razões dessa variabilidade são parcialmente conhecidas na expressão fenotípica da doença. Apesar de ter um mesmo defeito genético, a anemia falciforme pode estar associada com níveis diferentes de Hb Fetal e interações com talassemia alfa que atuam como modeladores genéticos da doença. Entretanto, outros defeitos genéticos dos eritrócitos, com destaques para a deficiência de G-6PD, a esferocitose e as deficiências de enzimas anti-oxidantes (SOD, GPx e catalase certamente interferem no curso clínico da doença. Os diferentes haplótipos da Hb S denominados por Banto, Benin, Senegal, Camarões e Asiático, tem sido apontados também como possíveis causas da heterogeneidade fenotípica da anemia falciforme. Toda essa diversidade que caracteriza a anemia falciforme está, em parte, relacionada à sua origem multicêntrica e que envolvem populações com diferentes anormalidades genéticas de proteínas e enzimas eritrocitárias. Por outro lado, além desses fatores caracterizados como interferentes eritrocitários, há os interferentes do meio ambiente em que está inserido o doente com anemia falciforme. Entre os interferentes ambientais destacam-se as situações sociais, econômicas e culturais do doente, e que tem influência no curso de sua doença. Diante desse quadro complexo e interativo, o presente artigo mostra a influência de certos interferentes eritrocitários e ambientais na anemia falciforme. Ao finalizar o artigo é proposto um protocolo de monitoramento laboratorial das síndromes falcêmicas, com destaque para a anemia falciforme.

Histamine combined with melphalan in isolated limb perfusion for the treatment of locally advanced soft tissue sarcomas: preclinical studies in rats Histamina combinada ao melfalano na perfusão de membro isolado para o tratamento de sarcomas de partes moles localmente avançado: estudos pré-clínicos em ratos

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Flavia Brunstein

2005-08-01

Full Text Available PURPOSE: To evaluate the potential benefit of histamine combined with melphalan in the isolated limb perfusion (ILP as an alternative to TNF-alfa and melphalan combination, for the treatment of irressectable soft tissue sarcomas of the limbs in Brown Norway (BN rats. METHODS: 20 BN rats had small fragments of syngeneic BN-175 fibosarcoma inserted on the right hind limb. In 7-10 days the tumor reached a median diameter of 12-15 mm and they were randomly divided in four groups (sham, melphalan, histamine and escalating doses of histamine combined to melphalan being submitted to experimental ILP for 30 minutes. Tumors were measured daily with a caliper and the volume was calculated. RESULTS: Response curves showed a significant effect of the combination of histamine 200 mg/mL with melphalan, with 66% overall response, including 33% complete responses (pOBJETIVO: Avaliar o potencial benéfico da histamina combinada ao melfalano, na perfusão de membro isolado (PMI, como alternativa à combinação TNF-alfa mais melfalano, no tratamento de sarcomas de partes moles irressecaveis em extremidades, em ratos de linhagem Brown Norway (BN. MÉTODOS: 20 ratos BN foram submetidos a implantação de fragmentos de fibrosarcoma singênico BN-175 na pata traseira direita. Em cerca de 7-10 dias o tumor atingiu um diâmetro médio de 12-15 mm e foram aleatóriamente divididos em quatro grupos (controle, melfalano, histamina em doses progessivas combinada ao melfalano e histamina sendo submetidos a PMI experimental por 30 minutos. Os tumores foram então medidos diariamente com o uso de paquímetro e o volume tumoral calculado. RESULTADOS: As curvas de resposta mostram um efeito significativo da combinação de Histamina na concentração de 200 mg/mL ao melfalano, com 66% de resposta global incluindo 33% de respostas completas (p < 0.01. Não houve efeitos colaterais sistêmicos e localmente apenas edema leve e transitório nos animais tratados com histamine

Ultrasonic and radiographic study of laxity in hip joints of young dogs Estudo ultra-sonográfico e radiográfico da frouxidão articular em cães jovens

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B.D. Rocha

2007-02-01

Full Text Available In the present study, 22 hip joints of Whippet (four, Rottweiler (five and Labrador Retriever (two young dogs were evaluated with the aim of comparing the ultrasonic examination of coxofemoral joints with the radiographic evaluations, both distraction and conventional procedures, for the early diagnosis of passive laxity. The study was based on static ultrasonography, conventional radiography (CR and distraction radiography (DR methods. In order to quantify the relationship between the femur head and the acetabulum, the alpha (alpha and gamma (gamma angles were measured by ultrasonographic examination, the Norberg angle (NA was measured by CR, and the distraction index (DI was measured by DR. It was observed a negative correlation between angles alpha and gamma (r= -0,756; PAvaliaram-se 22 articulações coxofemorais de cães das raças Whippet (quatro cães, Rottweiler (cinco cães e Labrador Retriever (dois cães, com o objetivo de comparar o exame ultra-sonográfico da articulação coxofemoral de cães jovens com as avaliações radiográficas em distração e convencional, para a determinação precoce da frouxidão articular passiva. Utilizaram-se os métodos ultra-sonográfico estático, radiográfico convencional (MRC e radiográfico em distração (MRD. Para quantificar a relação entre a cabeça do fêmur e o acetábulo, foram medidos os ângulos alfa (alfa e gama (gama no exame ultra-sonográfico, o ângulo de Norberg (AN no MRC e o índice de distração (ID no MRD. Observou-se correlação negativa entre os ângulos alfa e gama (r= -0,756; P<0,001 e entre ID e AN (r= -0,474; P<0,026. Não se observou correlação entre os ângulos alfa e gama em relação ao ID e o AN (alfa e ID: r= -0,380; P<0,081; alfa e NA: r= 0,013; P<0,954; gama e ID: r= 0, 338; P<0,124; ângulo gama e AN: r= -0,192; P<0,391. O método ultra-sonográfico estático não se mostrou sensível para a detecção precoce da frouxidão passiva da articula

Eficácia terapêutica do interferon alfa em criança com hemangioma gigante craniofacial: relato de caso Therapeutical effectiveness of interferon alpha in a child with craniofacial giant hemangioma: case report

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Nilson Lopes da Fonseca Junior

2008-06-01

Full Text Available O hemangioma é o tumor benigno mais freqüente da infância. O hemangioma capilar geralmente apresenta-se como uma mancha ou tumoração violácea bem delimitada. O diagnóstico destas lesões é clínico. O hemangioma gigante é rara e extensa variação do hemangioma capilar, que geralmente ocorre em recém-nascidos e lactentes. Várias são as modalidades terapêuticas, como a injeção intralesional de corticóide, laserterapia, injeção intralesional de soluções esclerosantes, corticoterapia sistêmica, cirurgia, radioterapia e embolização. Novas modalidades terapêuticas têm sido desenvolvidas, com o objetivo de se obter melhores resultados e possibilitar o tratamento de lesões de difícil acesso cirúrgico e refratárias às modalidades terapêuticas utilizadas rotineiramente. Os melhores resultados tem sido obtidos com o interferon alfa. Este é um caso de uma paciente com três meses de idade, que apresentava desde o nascimento, tumoração arroxeada e amolecida em pálpebra superior do olho direito, lesões cutâneas planas e arroxeadas em região temporal e parietal direita. Realizada tomografia computadorizada de crânio evidenciando processo expansivo orbitário vascularizado com extensão para fossa média, seio cavernoso e fossa posterior. O tratamento inicial foi a corticoterapia oral durante quarenta dias, com redução progressiva por quatro semanas. Com o quadro praticamente inalterado, foi iniciado o tratamento com interferon alfa, na dose de 3.000.000 U/m², subcutâneo, três vezes por semana. Após 9 meses de tratamento, observa-se apenas uma pequena lesão orbitária residual. Neste caso, o interferon alfa apresentou-se como boa opção no tratamento do hemangioma gigante craniofacial.Hemangiomas are the most commom benign tumors of infancy. Capillary hemangioma generally is presented as a spot or well-defined purple lesion. The diagnosis of these tumors is based on physical examination. Giant hemangioma is a rare

Reversing anticoagulant effects of novel oral anticoagulants: role of ciraparantag, andexanet alfa, and idarucizumab

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Hu TY

2016-02-01

Full Text Available Tiffany Y Hu,1 Vaibhav R Vaidya,2 Samuel J Asirvatham2,31Mayo Medical School, 2Division of Cardiovascular Diseases, Department of Internal Medicine, 3Department of Pediatrics and Adolescent Medicine, Mayo Clinic, Rochester, MN, USAAbstract: Novel oral anticoagulants (NOACs are increasingly used in clinical practice, but lack of commercially available reversal agents is a major barrier for mainstream use of these therapies. Specific antidotes to NOACs are under development. Idarucizumab (aDabi-Fab, BI 655075 is a novel humanized mouse monoclonal antibody that binds dabigatran and reverses its anticoagulant effect. In a recent Phase III study (Reversal Effects of Idarucizumab on Active Dabigatran, a 5 g intravenous infusion of idarucizumab resulted in the normalization of dilute thrombin time in 98% and 93% of the two groups studied, with normalization of ecarin-clotting time in 89% and 88% patients. Two other antidotes, andexanet alfa (PRT064445 and ciraparantag (PER977 are also under development for reversal of NOACs. In this review, we discuss commonly encountered management issues with NOACs such as periprocedural management, laboratory monitoring of anticoagulation, and management of bleeding. We review currently available data regarding specific antidotes to NOACs with respect to pharmacology and clinical trials.Keywords: novel oral anticoagulant, dabigatran, idarucizumab, reversal

Parametri za procena na kvalitetot na polietilenska i na polipropilenska ambalaza i na gumeni zatvoraci nameneti za farmacevtski preparati

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Liljana Ugrinova

2002-03-01

Full Text Available Napraven e pregled na parametrite za procena na kvalitetot na polietilenska i na polipropilenska ambalaza i na gumeni zatvoraci nameneti za farmacevtski preparati. Za procena na kvalitetot na ispituvaniot materijal bea izvrseni fizicki, hemiski i bioloski ispituvanja spored postapkite dadeni vo Ph. Eur., DIN i spored DIN ISO standardite. Baranjata za kvalitet na ovoj vid ambalaza propisani spored Ph. Eur., DIN i DIN ISO standardite se razlikuvaat vo odnos na predvidenite parametri za fizicki, za hemiski i za bioloski ispituvanja. Isto taka, propisani se i razlicni granici na dozvoleno otstapuvanje na oddelni parametri.

Significados do trabalho e do dinheiro: Quais suas funções sociais?

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Sabrina Cavalcanti Barros

2018-03-01

Full Text Available Estudos sobre o trabalho e o dinheiro têm enfatizado a dimensão institucional desses fenômenos, destacando funções como a capacidade de interligar a satisfação das necessidades individuais e a manutenção da ordem social. Tais funções são ativadas no cotidiano dos indivíduos, sendo construídas socialmente na produção dos significados dos referidos fenômenos. Exploramos as funções que os significados do trabalho e do dinheiro cumpriram na vida dos operários da construção civil. Realizamos entrevistas semiestruturadas com 44 operários e desenvolvemos análise de conteúdo temática. Identificamos dois grandes eixos norteadores: inclusão e inserção sociais versus exclusão social e realização e sentido de utilidade versus degradação. Os resultados indicaram que as condições de trabalho precárias e a escassez de dinheiro estruturaram os modos de enfrentamento dessas realidades, na valorização dos benefícios alcançados, na naturalização e no conformismo, decorrentes da dureza e limitação de oportunidades experimentadas por esses trabalhadores. Limitações da pesquisa e sugestões são apontadas.

Viabilidade econômica do uso do sistema de irrigação por gotejamento na cultura da cebola Economic viability of the use of drip irrigation system on onion crop

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Renato Carvalho Vilas Boas

2011-08-01

Full Text Available Objetivou-se, com este estudo, avaliar a viabilidade econômica do uso do sistema de irrigação por gotejamento na cultura da cebola. O experimento foi conduzido em canteiros construídos a céu aberto, na área experimental do Departamento de Agricultura/ UFLA, no período de junho a outubro de 2008. O delineamento experimental utilizado foi em blocos casualizados, em esquema fatorial 2 x 6, com quatro repetições. Os tratamentos constituíram-se de duas cultivares de cebola, cultivar híbrida Optima F1 e cultivar não híbrida Alfa Tropical, e seis tensões da água no solo, 15, 25, 35, 45, 60 e 75 kPa. A análise econômica da lavoura irrigada foi fundamentada na teoria dos custos de produção e, considerando-se a metodologia aplicada, conclui-se que as despesas com os recursos variáveis foram as que mais oneraram o custo final da cebola em todos os tratamentos estudados. Os custos totais médios foram, inversamente, proporcionais às produtividades dos tratamentos de tensão da água no solo, para ambas as cultivares, indicando uma resposta à escala de produção. Na condição do experimento, a recomendação é de que se adote, como momento de irrigar, a tensão da água no solo de 15 kPa e, como cultivar o híbrido Optima F1, para que se obtenha maior rentabilidade na atividade produtiva. A irrigação por gotejamento na cultura da cebola, adotando-se as tensões da água no solo e as cultivares estudadas neste trabalho, é uma técnica economicamente viável.The objective of this study was to evaluate the economic feasibility of the use of drip irrigation system on onion crop. The experiment was set at open field beds in the experimental area of the Agricultural Department/UFLA, from June to October 2008. The experimental design was randomized blocks in factorial scheme 2 x 6, with four replicates. Treatments consisted of two onion cultivars, the hybrid Optima F1 and the non hybrid Alfa Tropical and six soil water tension values, 15

Adubação orgânica na produção, rendimento e composição do óleo essencial da alfavaca quimiotipo eugenol Organic fertilization in the production, yield and chemical composition of basil chemotype eugenol

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Luiz Antonio Biasi

2009-03-01

Full Text Available Este trabalho foi realizado na UFPR, município de Pinhais-PR, de outubro de 2005 a junho de 2006. Avaliou-se a resposta do Ocimum gratissimum à adubação orgânica com composto de esterco de carneiro, verificando a viabilidade dessa prática para o aumento no rendimento de biomassa, na quantidade e qualidade de seu óleo essencial. As mudas foram plantadas com espaçamento de 0,5 m entre plantas dentro das parcelas e 1 m entre as parcelas. Testaram-se 3 doses de adubação com composto orgânico (4; 8 e 12 kg m-2 e uma testemunha, com 5 repetições e 8 plantas por parcela. As colheitas foram realizadas aos 150 e 226 dias após o plantio, sendo avaliado o rendimento de massa fresca e massa seca total e de folhas, flores e caule, o teor de óleo essencial das folhas e flores e a sua composição por cromatógrafo a gás acoplado a um detector de massas (CG-MS. Houve diferença entre os tratamentos, quanto ao rendimento de biomassa na primeira colheita, onde o tratamento com 8 kg m-2 de composto orgânico foi superior à testemunha sem adubação, para a massa fresca de flores por planta e massa seca total e de flores por planta, mas não diferiu dos outros níveis de adubação. Na segunda colheita não houve diferença entre os tratamentos. Quanto ao rendimento de óleo essencial não houve diferença entre os tratamentos nas duas colheitas. Também não foram observadas grandes variações nos componentes do óleo essencial decorrente dos tratamentos aplicados. Entretanto, ocorreram variações na composição do óleo essencial quando extraído das folhas e das flores, sendo o teor médio de eugenol de 90,4% nas folhas e 80,8% nas flores, na colheita em março. Já na colheita em junho, houve uma redução do teor de eugenol nas folhas e uma elevação dos teores, principalmente de alfa trans trans farneseno, beta bisaboleno, beta cariofileno, germacrene D e alfa selineno.This work was carried out at the Universidade Federal do Parana

Study of forward elastic $pp$ scattering at $\\sqrt{s}=8\\text{ TeV}$ with the ALFA detector

CERN Document Server

Stark, Simon Holm; Hansen, Jørgen Beck

The elastic $pp$ scattering data analyzed in this thesis was taken at the LHC with the ALFA detector at $\\sqrt{s}=8\\text{ TeV}$ with a $\\beta^*=1\\text{ km}$ collision optics which gave access to the Coulomb-Nuclear-Interference region. An improved procedure was used to fit the observed number of elastic events as a function of the four-momentum transfer with a simulation based on theoretical expectations including experimental effects. The data can not exclude any of the investigated parametrizations for the modulus of the nuclear amplitude. The fit results for the total $pp$ cross section, $\\sigma_\\text{tot}$, and the ratio of the real to imaginary forward elastic scattering amplitude, $\\rho$, are: \\begin{align*} \\sigma_\\text{tot} &= 99.1\\pm 0.3 \\text{ (stat.)}\\pm1.6\\text{ (syst.)} \\text{ mb}\\;,\\\\ \\rho &= 0.166\\pm0.017\\text{ (stat.)}\\pm0.019\\text{ (syst.)}\\;, \\end{align*} which are in agreement with previous measurements. As part of the analysis, the detector distance to the beam was determined with ...

Identificação dos citoplasmas "S", "T" e "N" via PCR em populações de cebola no Vale do São Francisco.

OpenAIRE

SANTOS, C. A. F.; LEITE, D. L.; COSTA, N. D.; OLIVEIRA, V. R.; SANTOS, I. C. N.; RODRIGUES, M. A.

2009-01-01

A identificação do tipo de citoplasma em cebola foi facilitada com a publicação de primers de DNA específicos para os tipos "S", "T" e "N". O objetivo do presente trabalho foi identificar, por meio de marcadores moleculares, o tipo de citoplasma presente na cultivar de cebola BRS São Francisco e numa população experimental em desenvolvimento na Embrapa Semi-Árido, de forma a orientar o desenvolvimento de híbridos de cebola adaptados à região. Na amostra de 19 plantas da 'BRS Alfa São Francisc...

Načrtovan porod na domu

OpenAIRE

Todorović, Tamara; Takač, Iztok

2017-01-01

Izhodišča: Porod na domu je sicer star toliko kot človeštvo, pa vendar v veliki večini srednje in visoko razvitih držav prevladuje mnenje, da so zaradi nepredvidljivosti zapletov porodnišnice najbolj varno okolje za rojevanje. Kljub temu obstaja peščica držav, v katerih je porod na domu integriran v sistem zdravstvenega varstva (npr. Nizozemska, Velika Britanija, Kanada). Pri porodih na domu ločimo nenačrtovane in načrtovane porode na domu, slednje pa lahko nadalje razdelimo še na porode s sp...

DETERMINAÇÃO DA EFICÁCIA DE DIFERENTES SOLVENTES ORGÂNICOS NA DISSOLUÇÃO DOS CORANTES NATURAIS BIXINA E NORBIXINA

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MARCO AURÉLIO DA SILVA COUTINHO

2017-05-01

Full Text Available Resumo: Nos últimos anos, os pigmentos naturais de urucum têm sido substancialmente utilizados pelas indústrias de alimentos para dar cor a produtos tais como: massas alimentícias, cereais, produtos de panificação, gelados comestíveis, sobremesas, condimentos, etc. Dentre esses pigmentos destaca-se a bixina, um éster monometílico do ácido dicarbixílico alfa-norbixina pouco solúvel em óleo e anorbixina um caratenóide diácido carboxilico. Em virtude da crescente aplicação industrial  dos corantes naturais, este trabalho teve como objetivo determinar a solubilidade desses pigmentos em diferentes solventes, por meio de espectrofotometria UV-vis. Foram preparadas soluções sob as mesmas condições, utilizando-se os solventes: acetona, álcool etílico, álcool isopropílico, dimetilsulfóxido e clorofómio. A absorbância foi medida em função do comprimento de onda de cada solução por meio da análise dos espectros de UV-Vis, na região de 800 a 200 nm. Os dados obtidos referentes às análises no UV-Vis confirmaram a maior eficácia do Dimetilsulfóxido (DMSO na dissolução da bixinae  e da acetona para o norbixina  em relação aos outros solventes.

Confiabilidade da tradução da versão brasileira do questionário PedsQL - DREA para avaliação da qualidade de vida de crianças e adolescentes

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Marcos Thomazin Lopes

2015-06-01

Full Text Available Resumo Objetivo: Avaliar a confiabilidade da tradução e adaptação cultural do questionário Pediatric Quality of Life Inventory (Peds- QLTM - Doença Renal em Estágio Avançado (DREA - versão 3.0 - relato das crianças/adolescentes e relato dos pais para avaliação da Qualidade de Vida Relacionada à Saúde (QRVS de crianças brasileiras portadoras de DREA. Método: Utilizou-se a metodologia proposta pelos autores da versão original dos questionários, por meio da administração dos mesmos a um grupo de 24 crianças e adolescentes portadores de DREA em acompanhamento na Unidade de Nefrologia Pediátrica do Instituto da Criança - HCFMUSP e 32 cuidadores primários (CP. Resultados: A análise estatística inicial dos sete domínios do questionário resultou em alfa de Cronbach entre 0,39 e 0,89. Os domínios que apresentaram valores inferiores a 0,5 foram recalculados separando-os por faixas etárias, o que resultou em elevação do alfa de Cronbach, demonstrando a influência da faixa etária na percepção da qualidade de vida, no paciente portador de DREA. A avaliação geral do alfa de Cronbach, todavia, apontou 0,81 e 0,71 para os questionários destinados aos relatos dos pacientes e dos CP, respectivamente, demonstrando uma boa consistência interna. Conclusão: A versão brasileira do questionário é válida, confiável e útil na mensuração da QVRS das crianças e dos adolescentes com DREA, segundo os relatos dos pacientes e dos CP.

Adaptação transcultural e validação do World Health Organization Health and Work Performance Questionnaire para enfermeiros brasileiros

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Monica Chiodi-Toscano de Campos

2013-12-01

Full Text Available Estudo com objetivo de adaptar transculturalmente o instrumento Health and Work Performance Questionnaire (HPQ e avaliar as propriedades psicométricas da versão brasileira para enfermeiros. O processo de adaptação seguiu o Protocolo de Tradução da Organização Mundial da Saúde. Os dados para a avaliação das propriedades psicométricas foram coletados em um hospital público e de ensino de Ribeirão Preto em 2011. As propriedades psicométricas analisadas foram: validade de face e conteúdo (grupo de especialistas, confiabilidade pelo Alfa de Cronbach e estabilidade por teste-reteste. Na avaliação das propriedades psicométricas, a consistência interna da versão adaptada do HPQ, o alfa de cronbach foi de 0,94 para a seção A e 0,86 para a seção B do instrumento. Na análise das concordâncias da estabilidade teste-reteste, as concordâncias foram positivas e estatisticamente significativas. Dessa forma, a versão adaptada do HPQ mostrou-se válida e confiável na amostra estudada.

Benefit of adjuvant interferon alfa-2b (IFN-α) therapy in melanoma patients with high serum MMP-8 levels

DEFF Research Database (Denmark)

Vihinen, Pia; Tervahartiala, Taina; Sorsa, Timo

2015-01-01

Matrix metalloproteinases (MMPs) are important enzymes in tissue turnover and various inflammatory processes. In this study, it was evaluated whether serum MMP-8 can predict the response to adjuvant interferon alfa-2b (IFN-α) therapy in patients with operated high-risk cutaneous melanoma. Pre......-treatment sera from 460 patients with stage IIB-IIIC melanoma were analyzed for MMP-8. The patients were randomized after surgery to adjuvant IFN-α for 12 or 24 months (n = 313) or observation only (n = 147). The median serum MMP-8 level was used to classify the patients into a low MMP-8 (n = 232) and a high MMP......-8 (n = 228) group. In the high MMP-8 subgroup, IFN-α therapy significantly improved relapse-free survival (RFS). RFS was 36.8 months in patients with high MMP-8 levels receiving IFN-α therapy, whereas RFS for those with high MMP-8 levels with observation only was 10.6 months (P = 0.027). Median...

Stigma scale of epilepsy: validation process Escala de estigma na epilepsia: processo de validação

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Paula T. Fernandes

2007-06-01

Full Text Available PURPOSE: To validate a Stigma Scale of Epilepsy (SSE. METHODS: The SSE was completed by 40 adult with epilepsy attending an Outpatient Epilepsy Clinic at the University Hospital of UNICAMP, and by 40 people from the community. People were interviewed on an individual basis; a psychologist read the questions to the subjects who wrote the answers in a sheet. The procedure was the same for all the subjects and completion took around ten minutes. RESULTS: The SSE has 24 items. The internal consistency of the SSE showed alpha Cronbach’s coefficient 0.88 for the patients with epilepsy and 0.81 for the community. The overall mean scores of the Stigma Scale of Epilepsy formula were: 46 (SD=18.22 for patients and 49 (SD=13.25 for the community where a score of 0 would suggest no stigma, and 100 maximum stigma. DISCUSSION: The SSE has satisfactory content validity and high internal consistency. It allows the quantification of the perception of stigma by patients and people from community; this can then be used for interventional studies, such as mass media campaign in minimizing the negative facets of stigma.OBJETIVO: Validar a Escala de Estigma na Epilepsia (EEE. MÉTODO: A EEE foi aplicada em 40 pacientes adultos com o diagnóstico de Epilepsia do Ambulatório de Epilepsia do HC/Unicamp e em 40 pessoas da comunidade em geral. Os sujeitos foram entrevistados individualmente pelas psicólogas, que foram lendo as questões e anotando as respostas na escala. As condições de aplicação foram as mesmas e a aplicação durou cerca de 10 minutos. RESULTADOS: A EEE possui 24 itens. A consistência interna da EEE mostrou alfa de Cronbach, de 0,88 para os pacientes com epilepsia e 0,81 para a comunidade. As médias do valor geral de estigma obtido com a fórmula da EEE foram: 46 (DP=18,22 para os pacientes e 49 (DP=13,25 para a comunidade. DISCUSSÃO: A EEE possui uma satisfatória validade e alta consistência interna. Isso permite uma quantificação da

Estudo comparativo entre clonidina associada à bupivacaína e bupivacaína isolada em bloqueio de plexo cervical para endarterectomia de carótida Estudio comparativo entre la clonidina asociada a la bupivacaína y la bupivacaína aislada en bloqueo de plexo cervical para endarterectomía de carótida A comparative study between bupivacaine and clonidine associated with bupivacaine in cervical plexus block for carotid endarterectomy

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Walter Pinto Neto

2009-08-01

Full Text Available JUSTIFICATIVA E OBJETIVOS: O bloqueio de plexo cervical permite avaliação neurológica durante a endarterctomia, além de manter analgesia pós-operatória. A clonidina é agonista alfa2 com efeito analgésico em diferentes bloqueios. O objetivo deste estudo foi comparar o efeito analgésico da clonidina com bupivacaína em relação à bupivacaína isolada em bloqueio de plexo cervical. MÉTODO: Foram avaliados 30 pacientes de forma aleatória e duplamente-encoberta divididos em dois grupos: G1 recebeu 1,5 mg.kg-1 de bupivacaína a 0,375% associados a 150 ¼g de clonidina (2 mL e G2, 1,5 mg.kg-1 de bupivacaína a 0,375% associados à solução fisiológica (2 mL. Foram avaliados: frequência cardíaca e pressão arterial nos momentos 0 (bloqueio, 30, 60, 90 e 120 minutos; necessidade de complementação anestésica; momento para primeira complementação analgésica; quantidade de analgésico usada e intensidade da dor nos momentos 0 (término da operação, 30, 60, 120, 240 e 360 minutos. RESULTADOS: A complementação anestésica com lidocaína foi de 3,8 mL no G1 e 3,6 mL no G2 sem diferença estatística significativa. O momento para a primeira complementação foi de 302,6 ± 152,6 minutos no G1, e de 236,6 ± 132,9 minutos no G2, sem diferença significativa. Não houve diferença na dose de dipirona e tramadol usada. Não houve diferença na intensidade da dor entre os grupos. CONCLUSÃO: A associação de 150 ¼g de clonidina à bupivacaína em bloqueio de plexo cervical para endarterectomia de carótida não promoveu melhora significativa do efeito analgésico avaliado por intensidade da dor, primeira complementação analgésica e quantidade de analgésico complementar.JUSTIFICATIVA Y OBJETIVOS: El bloqueo de plexo cervical permite la evaluación neurológica durante la endarterectomía, además de mantener la analgesia postoperatoria. La clonidina es agonista alfa-2 con un efecto analgésico en diferentes bloqueos. El objetivo de

Sentir o sentido: a experiência do código nos “homeóstatos” de José-Alberto Marques

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Manuel Portela

2016-09-01

Full Text Available http://dx.doi.org/10.5007/1807-9288.2016v12n1p69 Este breve ensaio analisa a série de poemas Homeóstatos de José-Alberto Marques como motores textuais. Ao tornar o código numa componente do texto, os Homeóstatos tipificam o poema experimental como uma investigação sobre a linguagem, a escrita e a leitura. A espacialização do texto na página é ativada de modo a produzir efeitos gerativos, combinatórios e ideogramáticos. Simultaneamente textos e instruções para gerar textos, os Homeóstatos constituem um exemplo de uma poética informacional que tira partido da dinâmica entre poema e sistema da língua para explorar os atos de escrita e leitura como processamento de sinais.

Korzystny wpływ kwasów omega-3 na rozwój dziecka = The beneficial effect of omega-3 acids on child development

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Agnieszka Pluta

2016-07-01

Zakład Pielęgniarstwa Społecznego Collegium Medicum w Bydgoszczy Uniwersytetu Mikołaja Kopernika w Toruniu         Keywords: child development, omega-3acids, supplementation. Słowa kluczowe: rozwój dziecka,  kwasy omega-3, suplementacja.     Abstract   Fatty acids are one of the determinants of normal development of the child. A special role is attributed to omega-3 acids. Representative of this group of acids include alpha-linolenic acid, eicosapentaenoic acid and docosahexaenoic acid. The paper presents the biological activity of omega-3 with a particular focus on their impact on child development.       Streszczenie   Kwasy tłuszczowe są jednym z determinantów prawidłowego rozwoju dziecka. Szczególną rolę przypisuje się kwasom omega-3. Przedstawicielami tej grupy kwasów są kwas alfa-linolenowy, dokozaheksaenowy oraz eikozapentaenowy. W pracy przedstawiono aktywność biologiczną kwasów omega-3 ze szczególnym uwzględnieniem ich wpływu na rozwój dziecka.

Boosting of antioxidant defence by interferon -Alfa in irradiated bone marrow transplanted rats

International Nuclear Information System (INIS)

Ashry, O.M.; Hussein, E.M.; Salama, S.F.

2009-01-01

this work was conducted to point out the influence of the biological responses modifiers namely interferon-Alfa (IFN-α)and bone marrow transplantation (BMT)on boosting the antioxidant level and protection from oxidative stress in irradiated rats. BMT was given to male rats, 3 hours post gamma irradiation at the dose level pf 5 Gy, that were injected intra peritoneal with 30,000 units of crude mouse virus-induced IFN-α biweekly for 2 months. glutathione (GSH) has a significant role in cell protection from oxidative stress. excessive lipid peroxidation can cause increased GSH consumption, thus the content of GSH was measured in blood, liver, lung and spleen tissues. product of lipid peroxidation (MDA)was measured in parallel. serum total lipids, cholesterol as well as blood parameters as white blood cells (WBCS), erythrocytes count (RBCs), hemoglobin content (Hb) and hematocrit value (Hct % ) were monitored. results point out that all investigated blood parameters were significantly elevated, while MDA was depressed in serum and spleen by BMT alone after 2 months. irradiation significantly depressed all investigated blood parameters, GSH of different tissues and conversely elevated level of lipid peroxidation, total lipids and cholesterol. WBCs, RBCs, Hb,Ht, blood GSH and serum MDA of of irradiated animals receiving BMT were normalized and exhibited significant differences compared to the irradiated group . IFN-α combined with BMT reconstituted blood parameters, normalized blood GSH and MDA and significantly elevated tissue GSH concentration and depressed tissue MDA as well as serum total lipids and cholesterol, compared to the irradiated group

Reticulocyte dynamic and hemoglobin variability in hemodialysis patients treated with Darbepoetin alfa and C.E.R.A.: a randomized controlled trial.

Science.gov (United States)

Forni, Valentina; Bianchi, Giorgia; Ogna, Adam; Salvadé, Igor; Vuistiner, Philippe; Burnier, Michel; Gabutti, Luca

2013-07-22

In a simulation based on a pharmacokinetic model we demonstrated that increasing the erythropoiesis stimulating agents (ESAs) half-life or shortening their administration interval decreases hemoglobin variability. The benefit of reducing the administration interval was however lessened by the variability induced by more frequent dosage adjustments. The purpose of this study was to analyze the reticulocyte and hemoglobin kinetics and variability under different ESAs and administration intervals in a collective of chronic hemodialysis patients. The study was designed as an open-label, randomized, four-period cross-over investigation, including 30 patients under chronic hemodialysis at the regional hospital of Locarno (Switzerland) in February 2010 and lasting 2 years. Four subcutaneous treatment strategies (C.E.R.A. every 4 weeks Q4W and every 2 weeks Q2W, Darbepoetin alfa Q4W and Q2W) were compared with each other. The mean square successive difference of hemoglobin, reticulocyte count and ESAs dose was used to quantify variability. We distinguished a short- and a long-term variability based respectively on the weekly and monthly successive difference. No difference was found in the mean values of biological parameters (hemoglobin, reticulocytes, and ferritin) between the 4 strategies. ESAs type did not affect hemoglobin and reticulocyte variability, but C.E.R.A induced a more sustained reticulocytes response over time and increased the risk of hemoglobin overshooting (OR 2.7, p = 0.01). Shortening the administration interval lessened the amplitude of reticulocyte count fluctuations but resulted in more frequent ESAs dose adjustments and in amplified reticulocyte and hemoglobin variability. Q2W administration interval was however more favorable in terms of ESAs dose, allowing a 38% C.E.R.A. dose reduction, and no increase of Darbepoetin alfa. The reticulocyte dynamic was a more sensitive marker of time instability of the hemoglobin response under ESAs therapy

Análisis cuantitativo de cuarzo-alfa en mezclas de cemento portland anhidro y calcita sintética por difracción de rayos X con equipo Philips PW-1700

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Sagrera-Moreno, J. L.

1985-09-01

Full Text Available In this article there are explained the necessary steps to make up a quantitative analysis of alpha-quartz applying the technique of rays X diffraction, in artificial mixtures of anhydrous portland cement and synthetic calcite, which contents are known.

En el presente artículo se explican los pasos necesarios para realizar un análisis cuantitativo de cuarzo-alfa aplicando la técnica de difracción de rayos X en mezclas artificiales de cemento portland anhidro y calcita sintética, cuyos contenidos son conocidos.

Comparative therapeutic effects of velaglucerase alfa and imiglucerase in a Gaucher disease mouse model.

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You-Hai Xu

2010-05-01

Full Text Available Gaucher disease type 1 is caused by the defective activity of the lysosomal enzyme, acid beta-glucosidase (GCase. Regular infusions of purified recombinant GCase are the standard of care for reversing hematologic, hepatic, splenic, and bony manifestations. Here, similar in vitro enzymatic properties, and in vivo pharmacokinetics and pharmacodynamics (PK/PD and therapeutic efficacy of GCase were found with two human GCases, recombinant GCase (CHO cell, imiglucerase, Imig and gene-activated GCase (human fibrosarcoma cells, velaglucerase alfa, Vela, in a Gaucher mouse, D409V/null. About 80+% of either enzyme localized to the liver interstitial cells and <5% was recovered in spleens and lungs after bolus i.v. injections. Glucosylceramide (GC levels and storage cell numbers were reduced in a dose (5, 15 or 60 U/kg/wk dependent manner in livers (60-95% and in spleens ( approximately 10-30%. Compared to Vela, Imig (60 U/kg/wk had lesser effects at reducing hepatic GC (p = 0.0199 by 4 wks; this difference disappeared by 8 wks when nearly WT levels were achieved by Imig. Anti-GCase IgG was detected in GCase treated mice at 60 U/kg/wk, and IgE mediated acute hypersensitivity and death occurred after several injections of 60 U/kg/wk (21% with Vela and 34% with Imig. The responses of GC levels and storage cell numbers in Vela- and Imig-treated Gaucher mice at various doses provide a backdrop for clinical applications and decisions.

Virus de la inmunodeficiencia felina (VIF): evaluación de las globulinas en pacientes infectados espontáneamente Feline immunodeficiency virus (FIV): study of globulins in patients with natural infections

OpenAIRE

N Gómez; M. A. Gisbert; L Ramayo; A Bratanich; V Castillo; A Suraniti

2006-01-01

Fueron estudiadas las posibles correlaciones de parámetros tales como la Alfa glicoproteína ácida (AGP, proteína de fase aguda), fracciones electroforéticas de las proteínas séricas y títulos de Toxoplasma gondii en gatos infectados por el Virus de Inmunodeficiencia Felina (VIF). Los títulos de Toxoplasma gondii obtenidos por Inmunofluorescencia Indirecta (IFI) no correlacionaron con los valores de Proteínas Totales ni con los de las globulinas. Sí se halló múltiple correlación entre todas la...

Effect of the medium composition on formation of amylase by Bacillus sp

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Eliana de Oliveira. Santos

2003-01-01

Full Text Available Studies on the alpha -amylase synthesis was carried out with a moderately thermophilic, facultatively anaerobic Bacillus sp, isolated from soil samples. The cells were cultivated in a complex medium containing soluble starch or maltose as carbon source. The levels of the alpha -amylaseactivity detected in culture supernatants varied greatly with the type of carbon source used. Maltose, soluble starch and citrate stimulated alpha -amylaseformation. Addition of exogenous glucose repressed formation of alpha -amylase, demonstrating that a classical glucose effect was operative in this organism. The concentration of yeast extract was found to be important factor in the alpha -amylase synthesis bythe isolate.The activity of the enzyme increased between 2 and 5 g/L yeast extract concentration and then fell very rapidly beyond this point. The best concentration of peptone to alpha-amylase formation was found to be around 10g/L.Estudos sobre a síntese de alfa -amilase foram realizados com uma bactéria termofílica moderada e facultativa anaeróbica, isolada de amostras de solo. As células foram cultivadas em um meio complexo contendo amido solúvel ou maltose como fonte de carbono. Os níveis da atividade de alfa -amilase detectados no sobrenadante da cultura variaram grandemente com o tipo da fonte de carbono utilizada. Amido solúvel, maltose e citrato estimularam a formação de alfa -amilase. A adição de glicose as culturas reprimiu a formação da alfa -amilase, demonstrando que o clássico efeito glicose foi operativo neste organismo. A concentração de extrato de levedura foi um fator importante na formação de alfa -amilase pelo isolado. A atividade da enzima aumentou entre concentrações de 2 a 5 g/L e então caiu muito rapidamente em torno deste ponto. A melhor concentração de peptona para a formação da alfa -amilase foi em torno de 10 g/L.

Multicenter, noninterventional, post-marketing surveillance study to evaluate dosing of recombinant human follicle-stimulating hormone using the redesigned follitropin alfa pen in women undergoing ovulation induction

Science.gov (United States)

Nawroth, Frank; Tandler-Schneider, Andreas; Bilger, Wilma

2015-01-01

This prospective, noninterventional, post-marketing surveillance study evaluated doses of recombinant human follicle-stimulating hormone (r-hFSH) using the redesigned follitropin alfa pen in women who were anovulatory or oligomenorrheic and undergoing ovulation induction (OI) alone or OI with intrauterine insemination. The primary endpoint was the proportion of patients who achieved monofollicular or bifollicular development (defined as one or two follicles ≥15 mm). Secondary endpoints included characteristics of ovulation stimulation treatment, such as mean total and mean daily r-hFSH doses. Data were analyzed for 3,193 patients from 30 German fertility centers. The proportion of patients with monofollicular or bifollicular development was 71.1% (n=2,270 of a total of 3,193 patients; intent-to-treat population). The mean±standard deviation total and daily doses of r-hFSH were 696.9±542.5 IU and 61.7±29.4 IU, respectively. The three doses prescribed most frequently were: 37.5 IU (n=703 from N=3,189; 22.0%), 50.0 IU (n=1,056 from N=3,189; 33.1%), and 75.0 IU (n=738 from N=3,189; 23.1%) on the first day of stimulation; and 37.5 IU (n=465 from N=3,189; 14.6%), 50.0 IU (n=922 from N=3,189; 28.9%), and 75.0 IU (n=895 from N=3,189; 28.1%) on the last day of stimulation. This noninterventional, post-marketing surveillance study found that monofollicular or bifollicular development was achieved in 71% of patients studied and the small dose increment (12.5 IU) of the redesigned follitropin alfa pen allowed individualized treatment of women undergoing OI. PMID:25926755

Hodnoceni reliability znalostních a psychologických testů

Czech Academy of Sciences Publication Activity Database

Martinková, Patrícia; Vlčková, Katarína

2014-01-01

Roč. 25, č. 4 (2014), s. 1-15 ISSN 1210-8022 Institutional support: RVO:67985807 Keywords : spolehlivost * reliabilita * znalostní testy * psychologické testy * Cronbachovo alfa * latentní proměnné * faktorová analýza * analýza rozptylu * logistická regrese * teorie odpovědi na položku Subject RIV: BB - Applied Statistics, Operational Research

Use of activated protein C has no avail in the early phase of acute pancreatitis.

Science.gov (United States)

Akay, Sinan; Ozutemiz, Omer; Yenisey, Cigdem; Simsek, Nilufer Genc; Yuce, Gul; Batur, Yucel

2008-01-01

Sepsis and acute pancreatitis have similar pathogenetic mechanisms that have been implicated in the progression of multiple organ failure. Drotrecogin alfa, an analogue of endogenous protein C, reduces mortality in clinical sepsis. Our objective was to evaluate the early therapeutic effects of activated protein C (APC) in a rat model of acute necrotizing pancreatitis. Acute necrotizing pancreatitis was induced by intraductal injection of 5% Na taurocholate. Hourly bolus injections of saline or recombinant human APC (drotrecogin alfa) was commenced via femoral venous catheter four hours after the induction of acute pancreatitis. The experiment was terminated nine hours after pancreatitis induction. Animals in group one (n=20) had a sham operation while animals in group two (n=20) received saline and animals in group three (n=20) received drotrecogin alfa boluses after acute pancreatitis induction. Pancreatic tissue for histopathologic scores and myeloperoxidase, glutathione reductase, glutathione peroxidase, and catalase activities were collected, and blood for serum amylase, urea, creatinine, and interleukin-6 measurements was withdrawn. Serum amylase activity was significantly lower in the APC treated group than the untreated group (17,435+/-432 U/L vs. 27,426+/-118 U/L, respectively). While the serum interleukin-6 concentration in the APC untreated group was significantly lower than the treated group (970+/-323 pg/mL vs. 330+/-368 pg/mL, respectively). In the early phase of acute pancreatitis, drotrecogin alfa treatment did not result in a significant improvement in oxidative and inflammatory parameters or renal functions.

Oxygenation of a rodent adenocarcinoma is improved by darbepoetin alfa

International Nuclear Information System (INIS)

Kirkpatrick, J.P.; Snyder, S.A.; Zhao, Y.; Peltz, C.; Brizel, D.M.; Dewhirst, M.W.; Blackwell, K.L.

2003-01-01

Full text: Tumor hypoxia, particularly in anemia, increases the risk of distant metastases and decreases survival. However, the benefits of stimulating erythropoiesis to supraphysiological levels are unclear. This study examines the effect of darbepoetin alfa (DEPA) on tumor oxygenation and the response to therapeutic radiation (XRT) in rats with initially normal hematocrit. R3230 adenocarcinoma was transplanted into the flank of female Fisher 344 rats. Starting on Day 4 post transplant, the rats received DEPA 3 μg/kg or normal saline sc tiw. At 3 weeks post transplant, pO2 was measured and the tumor excised, or the tumor was irradiated. pO2 was determined using an optical microprobe. In histologic studies, rats were injected with pimonidazole prior to sacrifice, and the tumors excised and stained with an antibody to pimonidazole (PIMO). Extent/intensity of staining was assessed by 3 observers and averaged to yield an IHC score. Rats ± DEPA with tumors 7 -12mm diameter were randomised to receive 32 Gy in 4 bid doses or sham irradiation. Tumor diameters in the 4 groups (±DEPA x ±XRT, total n=41) were measured by calipers. Failure was defined by the tumor reaching 15mm in diameter and used to generate Kaplan-Meier curves. Hematocrit at transplant averaged 45% (n=104) versus 67% in the DEPA-treated rats (n=18, p<0.0001) and 44% in the control animals (n=21.) Median pO2 in the DEPA-treated rats vs. controls did not differ significantly (17 vs. 13 mm Hg, p=0.10.) However, the fraction of tumor pO2 measurements <10mm Hg in the DEPA-treated rats was lower (21% vs. 37%, p=0.046.) IHC score was also less in the DEPA group (p=0.03.) XRT significantly improved failure-free survival (p<0.0001.) While the addition of DEPA to either the XRT or control groups did not significantly increase survival, the trend in both cases favored the DEPA-treated groups. In this tumor, treatment with DEPA improves oxygenation. However, no significant enhancement in radioesponsiveness by DEPA

Temperature variation in pulp chamber during dental bleaching in presence or absence of light activation = Variação da temperatura na câmara pulpar durante o clareamento dental na presença ou ausência de fotoativação

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Mollica, Fernanda Brandão

2010-01-01

Full Text Available Objetivo: Além da agressão química devido à penetração dos géis clareadores na polpa durante o clareamento de dentes com vitalidade pulpar, outro possível fator agressor pode ser o calor gerado pela reação de oxidação exotérmica do gel clareador, que pode também ser agravada pela fotoativação. Este estudo avaliou a variação da temperatura na câmara pulpar de dentes humanos, utilizando-se três diferentes géis clareadores, com ou sem fotoativação. Metodologia: Trinta pré-molares humanos foram cortados longitudinalmente para obtenção de duas metades: vestibular e lingual. Os 60 espécimes foram divididos em 3 grupos e os agentes clareadores utilizados variaram como segue: peróxido de hidrogênio 35% (WHP, peróxido de carbamida 37% (W e peróxido de hidrogênio 38% (OX. Metade dos espécimes foi submetida ao clareamento com fotoativação e, a outra metade, sem fotoativação. A fonte de luz utilizada foi o aparelho à base de diodo emissor de luz (LED, 3-Light, Clean Line e as temperaturas foram medidas por um termômetro digital. Os dados foram analisados por análise de variância e teste de Tukey (alfa=5%. Resultados: Os resultados de temperatura foram: sem fotoativação (WHP= 0. 68b; W= 0. 40b; OX= 0. 48b; com fotoativação (WHP= 2. 35a; W= 1. 60a; OX= 1. 80a. Conclusão: A fotoativação dos géis clareadores com LED contribuiu para um maior aumento de temperatura na câmara pulpar, mas não se atingiu a temperatura crítica de 5,5oC

Ornitina alfa-cetoglutarato na isquemia-reperfusÃo intestinal em ratos

OpenAIRE

Eduardo Silvio Gouveia GonÃalves

2009-01-01

Objetivo: Avaliar os efeito da ornitina α-cetoglutarato (OKG) no sangue e tecido intestinal de ratos submetidos à isquemia/reperfusÃo intestinal atravÃs da determinaÃÃo das concentraÃÃes in vivo no sangue e no tecido do intestino delgado, submetido a isquemia/reperfusÃo, de glicose, G 6 PDH, piruvato, acetoacetato, lactato, 3 HBDH, glutationa, T-Bars, mieloperoxidase, CPK e LDH. MÃtodo: Sessenta ratos (Rattus norvergicus albinus, Rodentia Mammalia) foram distribuÃdos aleatoriamente em ci...

Patrones de diversidad alfa en tres fragmentos de bosques montanos en la región norte de los Andes, Colombia

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Wilson López

2010-03-01

Full Text Available En el presente trabajo se analizaron los patrones de diversidad alfa en tres áreas protegidas de bosque con base en información florística proveniente de seis parcelas permanentes de 1ha -dos en cada área protegida-. Las regiones de estudio están localizadas a lo largo de un gradiente altitudinal, en la parte norte de la cordillera central de los Andes, Colombia. Para los análisis, se generaron dos categorías de acuerdo al tamaño de los árboles: dosel (diámetro a la altura del pecho mayor o igual a 10cm y sotobosque (diámetro a la altura del pecho mayor o igual a un cm y menor a 10cm. Las curvas especies-área mostraron diferencias en riqueza para las dos categorías de tamaño en las tres regiones. Las curvas especies-individuos, en cambio, fueron independientes con respecto al tamaño de los individuos, excepto en la región de Belmira. La diversidad, medida con el índice alfa de Fisher, mostró una fuerte tendencia a la estabilización en muestras mayores a 1 000 individuos. Ni las curvas de especies-área ni las de especies-individuos fueron asintóticas; sin embargo, la tasa de acumulación de especies (z en Belmira alcanzó valores muy bajos (0.05 en comparación con Anorí y Angelópolis (0.25 en cada caso. El índice alfa de Fisher permitió además entender la magnitud de las diferencias relativas en diversidad entre regiones o tipos de bosque en gradientes altitudinales, aun cuando el tamaño de la muestra fue relativamente pequeño. Los resultados de este estudio soportan la limitación en dispersión como el principal mecanismo en estructurar las comunidades de especies a escalas muy finas, y un incremento continuo de procesos denso-dependientes en determinar la estructura de la comunidad en escalas espaciales más grandes. Igualmente, los resultados de este estudio proponen que la denso-dependencia tiende a aumentar positivamente con la altitud.Alpha diversity patterns in three fragmented forests of Northern Andes

Na+-stimulated ATPase of alkaliphilic halotolerant cyanobacterium Aphanothece halophytica translocates Na+ into proteoliposomes via Na+ uniport mechanism

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Soontharapirakkul Kanteera

2010-08-01

Full Text Available Abstract Background When cells are exposed to high salinity conditions, they develop a mechanism to extrude excess Na+ from cells to maintain the cytoplasmic Na+ concentration. Until now, the ATPase involved in Na+ transport in cyanobacteria has not been characterized. Here, the characterization of ATPase and its role in Na+ transport of alkaliphilic halotolerant Aphanothece halophytica were investigated to understand the survival mechanism of A. halophytica under high salinity conditions. Results The purified enzyme catalyzed the hydrolysis of ATP in the presence of Na+ but not K+, Li+ and Ca2+. The apparent Km values for Na+ and ATP were 2.0 and 1.2 mM, respectively. The enzyme is likely the F1F0-ATPase based on the usual subunit pattern and the protection against N,N'-dicyclohexylcarbodiimide inhibition of ATPase activity by Na+ in a pH-dependent manner. Proteoliposomes reconstituted with the purified enzyme could take up Na+ upon the addition of ATP. The apparent Km values for this uptake were 3.3 and 0.5 mM for Na+ and ATP, respectively. The mechanism of Na+ transport mediated by Na+-stimulated ATPase in A. halophytica was revealed. Using acridine orange as a probe, alkalization of the lumen of proteoliposomes reconstituted with Na+-stimulated ATPase was observed upon the addition of ATP with Na+ but not with K+, Li+ and Ca2+. The Na+- and ATP-dependent alkalization of the proteoliposome lumen was stimulated by carbonyl cyanide m - chlorophenylhydrazone (CCCP but was inhibited by a permeant anion nitrate. The proteoliposomes showed both ATPase activity and ATP-dependent Na+ uptake activity. The uptake of Na+ was enhanced by CCCP and nitrate. On the other hand, both CCCP and nitrate were shown to dissipate the preformed electric potential generated by Na+-stimulated ATPase of the proteoliposomes. Conclusion The data demonstrate that Na+-stimulated ATPase from A. halophytica, a likely member of F-type ATPase, functions as an electrogenic Na

Polarization dependence of Na* + Na* associative ionization revisited

NARCIS (Netherlands)

Meijer, H.A.J.; Meulen, H.P. v.d.; Morgenstern, R.; Hertel, I.V.; Meyer, E.; Witte, R.

1986-01-01

The dependence of the associative ionization process Na 3 2P3/2 + Na 3 2P3/2 → Na2+ + e- on the polarization of the laser light used for Na excitation was independently investigated in Utrecht and Berlin. The purpose of this paper is to clarify discrepancies between two other earlier experimental

The Portuguese language version of social phobia and Anxiety Inventory: analysis of items and internal consistency in a Brazilian sample of 1,014 undergraduate students Versão para o português do Inventário de Fobia Social e Ansiedade: análise de itens e consistência interna numa amostra de 1.014 estudantes universitários brasileiros

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Patrícia Picon

2006-01-01

Full Text Available OBJECTIVE: Theoretical and empirical analysis of items and internal consistency of the Portuguese-language version of Social Phobia and Anxiety Inventory (SPAI-Portuguese. METHODS: Social phobia experts conducted a 45-item content analysis of the SPAI-Portuguese administered to a sample of 1,014 university students. Item discrimination was evaluated by Student's t test; interitem, mean and item-to-total correlations, by Pearson coefficient; reliability was estimated by Cronbach's alpha. RESULTS: There was 100% agreement among experts concerning the 45 items. On the SPAI-Portuguese 43 items were discriminative (p OBJETIVO: Análise teórica e empírica dos itens e da consistência interna da versão em português do Social Phobia and Anxiety Inventory (SPAI-Português e subescalas. MÉTODOS: Peritos em fobia social conduziram análise de conteúdo dos 45 itens do SPAI-Português, administrado a 1.014 estudantes universitários. A discriminação dos itens foi avaliada por teste t de Student; correlações interitens, médias e item/total por coeficientes de Pearson; fidedignidade pelo alfa de Cronbach. RESULTADOS: Concordância plena entre os peritos para os 45 itens. SPAI-Português com 43 itens discriminativos (p < 0,05. Alguns itens, entre as subescalas, apresentaram coeficientes abaixo de 0,2. As médias das correlações interitens foram: 0,41 na subescala fobia social; 0,32 na subescala agorafobia; e 0,32 no SPAI-Português. As correlações item/total foram maiores do que 0,3 (p < 0,001. Alfas de Cronbach foram: 0,95 no SPAI-Português; 0,96 na subescala de fobia social; 0,85 na subescala de agorafobia. CONCLUSÃO: O conteúdo dos itens foi relacionado aos constructos subjacentes (agorafobia e fobia social, com discriminabilidade de 43 itens do SPAI-Português. As correlações médias interitens e alfas revelaram consistência interna de SPAI-Português e subescalas, além de multidimensionalidade das mesmas. Nenhum item foi suprimido

Efektivitas Penambahan Vitamin E (alfa-Tokoferol dalam Medium Pencucian Sperma dengan Sentrifugasi terhadap Kualitas Spermatozoa Sapi Brahman

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Dasrul Dasrul

2012-10-01

Full Text Available Effect of vitamin E addition (alfa-tokoferol into sperm washing medium by centrifuge on the quality of Brahman cattle spermatozoa  ABSTRACT. The aims of study to determine the effectiveness of the addition of vitamin E in the washing medium by centrifugation on sperm quality Brahman cattle. frozen semen of Brahman cattle, divided into 4 treatment groups addition of vitamin E in the washing medium: 0.0gr/100 ml medium (K0, 0.1gr /100 ml medium (K1; 0.2gr/100 ml medium (K2 and 0.3 g / 100 ml medium (K4, each group was repeated 5 times. Examination of motility, viability and integrity of sperm membrane performed according to WHO standards. The data obtained were analyzed with one-way ANOVA and Duncan test. The average percentage of motility, viability and membrane integrity of spermatozoa in the addition of vitamin E were significantly different (P 0.05 compared with the group K0. The addition of vitamin E in the medium on the process of washing spermatozoa Brahman cattle. The addition of vitamin E 0.2gr/100ml better than vitamin E 0.1gr/100ml and 0.3gr/100ml in maintaining the percentage of motility and live spermatozoa Brahman cattle.

ESI mass spectrometry applied to enantiodiscrimination of chiral systems

International Nuclear Information System (INIS)

Lagana, A.; Paladini, A.; Catone, D.; Giardini, A.; Filippi, A.; D'Ettole, A.; Speranze, M.

2002-01-01

Experiments with electrospray ionization coupled with mass spectrometric detection (ESI-MS) together with collisional induced dissociation (CID) were performed in order to study the affinity of chiral alfa-aminophosphonic acids towards first-group metals (Na, Li, K) in gaseous phase and how this can be affected by the ligands configuration. The results are discussed in the light of structure calculation performed by using an empirical force field. The CID fragmentation spectra of diastereomeric clusters containing (1-amino-2-methylpropyl)phosphonic acid enantiomers (P R , P S ), (S)-(+)-(1-aminoethyl)phosphonic acid (E S ) and a sodium ion ( [NaP R (E S ) 2 ] + and [NaP S (E S ) 2 ] + ) is given. (nevyjel)

Pseudo-ternary phase diagram in the Na2O-Na2O2-NaOH system

International Nuclear Information System (INIS)

Saito, Jun-ichi; Tendo, Masayuki; Aoto, Kazumi

1997-10-01

Generally, the phase diagrams are always used to understand the present state of compounds at certain temperature. In order to understand the corrosion behavior of structural material for FBR by main sodium compounds (Na 2 O, Na 2 O 2 and NaOH), it is very important to comprehend the phase diagrams of their compounds. However, only Na 2 O-NaOH pseudo-binary phase diagram had been investigated previously in this system. There is no study of other pseudo-binary or ternary phase diagrams in the Na 2 O-Na 2 O 2 -NaOH system. In this study, in order to clarify the present states of their compounds at certain temperatures, the pseudo-binary and ternary phase diagrams in the Na 2 O-Na 2 O 2 -NaOH system were prepared. A series of thermal analyses with binary and ternary component system has been carried out using the differential scanning calorimetry (DSC). The liquidus temperature and ternary eutectic temperatures were confirmed by these measurements. The beneficial indications for constructing phase diagrams were obtained from these experiments. On the basis of these results, the interaction parameters between compounds which were utilized for the Thermo-Calc calculation were optimized. Thermo-Calc is one of thermodynamic calculation software. Consequently the accurate pseudo-binary and ternary phase diagrams were indicated using the optimized parameters. (author)

Extracellular Na+ levels regulate formation and activity of the NaX/alpha1-Na+/K+-ATPase complex in neuronal cells.

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Emmanuelle eBerret

2014-12-01

Full Text Available MnPO neurons play a critical role in hydromineral homeostasis regulation by acting as sensors of extracellular sodium concentration ([Na+]out. The mechanism underlying Na+-sensing involves Na+-flow through the NaX channel, directly regulated by the Na+/K+-ATPase α1-isoform which controls Na+-influx by modulating channel permeability. Together, these two partners form a complex involved in the regulation of intracellular sodium ([Na+]in. Here we aim to determine whether environmental changes in Na+ could actively modulate the NaX/Na+/K+-ATPase complex activity.We investigated the complex activity using patch-clamp recordings from rat MnPO neurons and Neuro2a cells. When the rats were fed with a high-salt-diet, or the [Na+] in the culture medium was increased, the activity of the complex was up-regulated. In contrast, drop in environmental [Na+] decreased the activity of the complex. Interestingly under hypernatremic condition, the colocalization rate and protein level of both partners were up-regulated. Under hyponatremic condition, only NaX protein expression was increased and the level of NaX/Na+/K+-ATPase remained unaltered. This unbalance between NaX and Na+/K+-ATPase pump proportion would induce a bigger portion of Na+/K+-ATPase-control-free NaX channel. Thus we suggest that hypernatremic environment increases NaX/Na+/K+-ATPase α1-isoform activity by increasing the number of both partners and their colocalization rate, whereas hyponatremic environment down-regulates complex activity via a decrease in the relative number of NaX channels controlled by the pump.

Aglutinina anti-T (Thomsen-Friedenreich na doença de Chagas

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Marcos Palatnik

1986-10-01

Full Text Available Uma amostra de soros de pacientes e controles de uma área endêmica da Doença de Chagas do País foi titulada contra hemácias de grupo O tratadas pela neuraminidase (células OT ativadas e hemacias normais de grupo A e B (sistema ABO. Também foram feitos alguns testes de aglutinação de hemácias de pacientes com cardiomiopatia com as lectinas de amendoim (anti-T + anti-Tk e com anticorpo humano anti-T. O escore médio da aglutinina anti-T dos pacientes foi similar ao do grupo controle. En- tretanto, o escore médio dos indivíduos com a forma cardíaca íoi significantemente maior que o dos pacientes assintomáticos e também maior que o do grupo controle. A comparação entre indivíduos com sorologia diagnóstica positiva versus negativa foi não significante. Nem a sorologia para Chagas nem as variáveis raça, sexo, idade, idade ao quadrado, in-fecção intestinal parasitária, teor de proteínas do soro, volume globular eritrocitário (hema-tócrito, grau de palidez da mucosa bucal, cardíomiopatia e os termos de interação sexo x idade, sexo x idade ao quadrado, estão associados significativamente com o logaritmo do título ou com o logaritmo do escore de aglutinação. Os escores de aglutinação das aglutininas anti-A e anti-B do sistema de grupo sangüíneo ABO estão dentro da faixa de variação normal. As hemácias de pacientes com a forma cardíaca não são aglutinadas nem pelas lectinas de amendoim (antiT + anti-Tk e da soja (anti-T nem pela aglutinina anti-T de origem humana. A prova de Coombs direta foi negativa em todos os pacientes, demonstrando a aparente ausência de imunoglobulinas IgG e IgM na superfície eritrocitária. O fato de que o aumento dos níveis de anti-T só foi detectado em uma das formas clínicas da doença em pacientes da mesma área endêmica constitui um argumento contra a possibilidade de que a variação observada seja produzida por uma infecção concomitante.

A Study of the Distribution of Sodium Cations in the Zeolites NaX, NaY and ZnNaY Using Carbon Monoxide Adsorption and 23Na NMR Techniques

Science.gov (United States)

Seidel, A.; Boddenberg, B.

1995-03-01

The zeolites NaX, NaY, Zn(55)NaY, and Zn(74)NaY were investigated by means of carbon monoxide adsorption and with static and magic angle spinning (MAS) 23Na NMR spectroscopy. The Na+ distribution between the sodalite (ß)- and supercages of the fully hydrated zeolites NaX and NaY were found to agree with XRD results. In the hydrated zinc-exchanged zeolites the Na+ ions almost exclusively populate the ß-cages. The adsorption isotherms of CO in the dehydrated zeolites were analyzed quantitatively to yield the concentrations of Na+ residing in the supercages. The measured static and MAS 23Na NMR spectra were analyzed by comparing their widths and shapes with simulated central transition patterns and yield, inter alia, the concentrations of Na+ associated with the spectrum components. Arguments are put forward that 23Na NMR of dehydrated zeolites is well suited to distinguish Na+ cations in highly symmetric environments and mobile Na+ species from others located on general positions, but further resolution is hardly feasible.

HCO3(-)-coupled Na+ influx is a major determinant of Na+ turnover and Na+/K+ pump activity in rat hepatocytes

International Nuclear Information System (INIS)

Fitz, J.G.; Lidofsky, S.D.; Weisiger, R.A.; Xie, M.H.; Cochran, M.; Grotmol, T.; Scharschmidt, B.F.

1991-01-01

Recent studies in hepatocytes indicate that Na(+)-coupled HCO3- transport contributes importantly to regulation of intracellular pH and membrane HCO3- transport. However, the direction of net coupled Na+ and HCO3- movement and the effect of HCO3- on Na+ turnover and Na+/K+ pump activity are not known. In these studies, the effect of HCO3- on Na+ influx and turnover were measured in primary rat hepatocyte cultures with 22Na+, and [Na+]i was measured in single hepatocytes using the Na(+)-sensitive fluorochrome SBFI. Na+/K+ pump activity was measured in intact perfused rat liver and hepatocyte monolayers as Na(+)-dependent or ouabain-suppressible 86Rb uptake, and was measured in single hepatocytes as the effect of transient pump inhibition by removal of extracellular K+ on membrane potential difference (PD) and [Na+]i. In hepatocyte monolayers, HCO3- increased 22Na+ entry and turnover rates by 50-65%, without measurably altering 22Na+ pool size or cell volume, and HCO3- also increased Na+/K+ pump activity by 70%. In single cells, exposure to HCO3- produced an abrupt and sustained rise in [Na+]i from approximately 8 to 12 mM. Na+/K+ pump activity assessed in single cells by PD excursions during transient K+ removal increased congruent to 2.5-fold in the presence of HCO3-, and the rise in [Na+]i produced by inhibition of the Na+/K+ pump was similarly increased congruent to 2.5-fold in the presence of HCO3-. In intact perfused rat liver, HCO3- increased both Na+/K+ pump activity and O2 consumption. These findings indicate that, in hepatocytes, net coupled Na+ and HCO3- movement is inward and represents a major determinant of Na+ influx and Na+/K+ pump activity. About half of hepatic Na+/K+ pump activity appears dedicated to recycling Na+ entering in conjunction with HCO3- to maintain [Na+]i within the physiologic range

The effect of Na vapor on the Na content of chondrules

Science.gov (United States)

Lewis, R. Dean; Lofgren, Gary E.; Franzen, Hugo F.; Windom, Kenneth E.

1993-01-01

Chondrules contain higher concentrations of volatiles (Na) than expected for melt droplets in the solar nebula. Recent studies have proposed that chondrules may have formed under non-canonical nebular conditions such as in particle/gas-rich clumps. Such chondrule formation areas may have contained significant Na vapor. To test the hypothesis of whether a Na-rich vapor would minimize Na volatilization reaction rates in a chondrule analog and maintain the Na value of the melt, experiments were designed where a Na-rich vapor could be maintained around the sample. A starting material with a melting point lower that typical chondrules was required to keep the logistics of working with Na volatilization from NaCl within the realm of feasibility. The Knippa basalt, a MgO-rich alkali olivine basalt with a melting temperature of 1325 +/- 5 C and a Na2O content of 3.05 wt%, was used as the chondrule analog. Experiments were conducted in a 1 atm, gas-mixing furnace with the fO2 controlled by a CO/CO2 gas mixture and fixed at the I-W buffer curve. To determine the extent of Na loss from the sample, initial experiments were conducted at high temperatures (1300 C - 1350 C) for duration of up to 72 h without a Na-rich vapor present. Almost all (up to 98%) Na was volatilized in runs of 72 h. Subsequent trials were conducted at 1330 C for 16 h in the presence of a Na-rich vapor, supplied by a NaCl-filled crucible placed in the bottom of the furnace. Succeeding Knudsen cell weight-loss mass-spectrometry analysis of NaCl determined the P(sub Na) for these experimental conditions to be in the 10(exp -6) atm range. This value is considered high for nebula conditions but is still plausible for non-canonical environments. In these trials the Na2O content of the glass was maintained or in some cases increased; Na2O values ranged from 2.62% wt to 4.37% wt. The Na content of chondrules may be controlled by the Na vapor pressure in the chondrule formation region. Most heating events capable

Arecibo pulsar survey using ALFA. III. Precursor survey and population synthesis

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Swiggum, J. K.; Lorimer, D. R.; McLaughlin, M. A.; Bates, S. D.; Senty, T. R. [Department of Physics and Astronomy, West Virginia University, Morgantown, WV 26506 (United States); Champion, D. J.; Lazarus, P. [Max-Planck-Institut für Radioastronomie, D-53121 Bonn (Germany); Ransom, S. M. [NRAO, Charlottesville, VA 22903 (United States); Brazier, A.; Chatterjee, S.; Cordes, J. M. [Astronomy Department, Cornell University, Ithaca, NY 14853 (United States); Hessels, J. W. T. [ASTRON, Netherlands Institute for Radio Astronomy, Postbus 2, 7990 AA, Dwingeloo (Netherlands); Nice, D. J. [Department of Physics, Lafayette College, Easton, PA 18042 (United States); Ellis, J.; Allen, B. [Physics Department, University of Wisconsin-Milwaukee, Milwaukee WI 53211 (United States); Bhat, N. D. R. [Center for Astrophysics and Supercomputing, Swinburne University, Hawthorn, Victoria 3122 (Australia); Bogdanov, S.; Camilo, F. [Columbia Astrophysics Laboratory, Columbia University, New York, NY 10027 (United States); Crawford, F. [Department of Physics and Astronomy, Franklin and Marshall College, Lancaster, PA 17604-3003 (United States); Deneva, J. S. [Arecibo Observatory, HC3 Box 53995, Arecibo, PR 00612 (United States); and others

2014-06-01

The Pulsar Arecibo L-band Feed Array (PALFA) Survey uses the ALFA 7-beam receiver to search both inner and outer Galactic sectors visible from Arecibo (32° ≲ ℓ ≲ 77° and 168° ≲ ℓ ≲ 214°) close to the Galactic plane (|b| ≲ 5°) for pulsars. The PALFA survey is sensitive to sources fainter and more distant than have previously been seen because of Arecibo's unrivaled sensitivity. In this paper we detail a precursor survey of this region with PALFA, which observed a subset of the full region (slightly more restrictive in ℓ and |b| ≲ 1°) and detected 45 pulsars. Detections included 1 known millisecond pulsar and 11 previously unknown, long-period pulsars. In the surveyed part of the sky that overlaps with the Parkes Multibeam Pulsar Survey (36° ≲ ℓ ≲ 50°), PALFA is probing deeper than the Parkes survey, with four discoveries in this region. For both Galactic millisecond and normal pulsar populations, we compare the survey's detections with simulations to model these populations and, in particular, to estimate the number of observable pulsars in the Galaxy. We place 95% confidence intervals of 82,000 to 143,000 on the number of detectable normal pulsars and 9000 to 100,000 on the number of detectable millisecond pulsars in the Galactic disk. These are consistent with previous estimates. Given the most likely population size in each case (107,000 and 15,000 for normal and millisecond pulsars, respectively), we extend survey detection simulations to predict that, when complete, the full PALFA survey should have detected 1000{sub −230}{sup +330} normal pulsars and 30{sub −20}{sup +200} millisecond pulsars. Identical estimation techniques predict that 490{sub −115}{sup +160} normal pulsars and 12{sub −5}{sup +70} millisecond pulsars would be detected by the beginning of 2014; at the time, the PALFA survey had detected 283 normal pulsars and 31 millisecond pulsars, respectively. We attribute the deficiency in normal pulsar

Effects of alfa-hydroxy-isocaproic acid on body composition, DOMS and performance in athletes

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Karila Tuomo AM

2010-01-01

Full Text Available Abstract Background Alfa-Hydroxy-isocaproic acid (HICA is an end product of leucine metabolism in human tissues such as muscle and connective tissue. According to the clinical and experimental studies, HICA can be considered as an anti-catabolic substance. The present study investigated the effects of HICA supplementation on body composition, delayed onset of muscle soreness (DOMS and physical performance of athletes during a training period. Methods Fifteen healthy male soccer players (age 22.1+/-3.9 yr volunteered for the 4-week double-blind study during an intensive training period. The subjects in the group HICA (n = 8 received 583 mg of sodium salt of HICA (corresponding 500 mg of HICA mixed with liquid three times a day for 4 weeks, and those in the group PLACEBO (n = 7 received 650 mg of maltodextrin mixed with liquid three times a day for the same period. According to a weekly training schedule, they practiced soccer 3 - 4 times a week, had strength training 1 - 2 times a week, and had one soccer game during the study. The subjects were required to keep diaries on training, nutrition, and symptoms of DOMS. Body composition was evaluated with a dual-energy X-ray absorptiometry (DXA before and after the 4-week period. Muscle strength and running velocity were measured with field tests. Results As compared to placebo, the HICA supplementation increased significantly body weight (p th week of the treatment (p Conclusion Already a 4-week HICA supplementation of 1.5 g a day leads to small increases in muscle mass during an intensive training period in soccer athletes.

Caracterização química e físico-química do óleo das sementes de Platymiscium floribundum Vog. (sacambu colhidas na fase de desenvolvimento e na época de maturação fisiológica. Chemical and chemical-physics characterization of seeds’ oil of Platymiscium floribundum Vog. (sacambu collected evolution state and stage of fruit’s maturation.

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Maria Isabel VALLILO

2007-12-01

Full Text Available Frutos de Platymiscium floribundum Vog. -Fabaceae (sacambu foram colhidos no ParqueEstadual Alberto Löfgren, na cidade de São Paulo,em agosto e novembro de 2004, correspondendo adiferentes condições climáticas e de amadurecimento.As sementes foram analisadas com o objetivo de seavaliar os teores de lipídios, ácidos graxos e tocoferóis,em duas épocas de colheita. Os lipídios foramextraídos com éter etílico por soxhlet. A identificaçãoe quantificação dos ácidos graxos e tocoferóis foramfeitas por cromatografia a gás (CG, com detetorde ionização de chama e cromatografia líquida dealta eficiência (CLAE, com detetor de fluorescência,respectivamente. Obteve-se maior teor de lipídios(23,6 g 100 g-1 no estádio de maturação fisiológicadas sementes. Não se observou diferenças relevantesno perfil de ácidos graxos nas duas épocas de colheita;no entanto, os teores de alfa e gama tocoferóis,expressos mg 100 g-1, apresentaram mudançasconsideráveis com o amadurecimento das sementes,havendo um aumento na concentração de 27,7 para42,8 alfa ( tocoferóis e de 68,9 para 88,1 gama( tocoferóis, o mesmo não ocorrendo com beta( e delta ( tocoferóis, que permanecerampraticamente inalterados com o amadurecimentodas sementes.Platymiscium floribundum Vog. - Fabaceae(sacambu fruits have been collected in the ParqueEstadual Alberto Löfgren in São Paulo city, in Augustand November 2004, corresponding both to differentclimatic conditions and of matureness. The seedshave been analyzed in order to determine lipids,fatty acids and tocopherols rates, in the twoseasonal maturation phases. The lipids have beenextracted with ethyl ether for soxhlet. Fatty acidsand tocopherol identification and quantificationhave been made by gas chromatograph (GC,with flame ionization detector and high performanceliquid chromatography (HPLC with fluorescencedetector, respectively. The results showed high levelof lipid (23.6 g 100 g-1 in the phase of

Na/K pump inactivation, subsarcolemmal Na measurements, and cytoplasmic ion turnover kinetics contradict restricted Na spaces in murine cardiac myocytes.

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Lu, Fang-Min; Hilgemann, Donald W

2017-07-03

Decades ago, it was proposed that Na transport in cardiac myocytes is modulated by large changes in cytoplasmic Na concentration within restricted subsarcolemmal spaces. Here, we probe this hypothesis for Na/K pumps by generating constitutive transsarcolemmal Na flux with the Na channel opener veratridine in whole-cell patch-clamp recordings. Using 25 mM Na in the patch pipette, pump currents decay strongly during continuous activation by extracellular K (τ, ∼2 s). In contradiction to depletion hypotheses, the decay becomes stronger when pump currents are decreased by hyperpolarization. Na channel currents are nearly unchanged by pump activity in these conditions, and conversely, continuous Na currents up to 0.5 nA in magnitude have negligible effects on pump currents. These outcomes are even more pronounced using 50 mM Li as a cytoplasmic Na congener. Thus, the Na/K pump current decay reflects mostly an inactivation mechanism that immobilizes Na/K pump charge movements, not cytoplasmic Na depletion. When channel currents are increased beyond 1 nA, models with unrestricted subsarcolemmal diffusion accurately predict current decay (τ ∼15 s) and reversal potential shifts observed for Na, Li, and K currents through Na channels opened by veratridine, as well as for Na, K, Cs, Li, and Cl currents recorded in nystatin-permeabilized myocytes. Ion concentrations in the pipette tip (i.e., access conductance) track without appreciable delay the current changes caused by sarcolemmal ion flux. Importantly, cytoplasmic mixing volumes, calculated from current decay kinetics, increase and decrease as expected with osmolarity changes (τ >30 s). Na/K pump current run-down over 20 min reflects a failure of pumps to recover from inactivation. Simulations reveal that pump inactivation coupled with Na-activated recovery enhances the rapidity and effectivity of Na homeostasis in cardiac myocytes. In conclusion, an autoregulatory mechanism enhances cardiac Na/K pump activity when

Na+/Ca2+ exchange and Na+/K+-ATPase in the heart

Science.gov (United States)

Shattock, Michael J; Ottolia, Michela; Bers, Donald M; Blaustein, Mordecai P; Boguslavskyi, Andrii; Bossuyt, Julie; Bridge, John H B; Chen-Izu, Ye; Clancy, Colleen E; Edwards, Andrew; Goldhaber, Joshua; Kaplan, Jack; Lingrel, Jerry B; Pavlovic, Davor; Philipson, Kenneth; Sipido, Karin R; Xie, Zi-Jian

2015-01-01

This paper is the third in a series of reviews published in this issue resulting from the University of California Davis Cardiovascular Symposium 2014: Systems approach to understanding cardiac excitation–contraction coupling and arrhythmias: Na+ channel and Na+ transport. The goal of the symposium was to bring together experts in the field to discuss points of consensus and controversy on the topic of sodium in the heart. The present review focuses on cardiac Na+/Ca2+ exchange (NCX) and Na+/K+-ATPase (NKA). While the relevance of Ca2+ homeostasis in cardiac function has been extensively investigated, the role of Na+ regulation in shaping heart function is often overlooked. Small changes in the cytoplasmic Na+ content have multiple effects on the heart by influencing intracellular Ca2+ and pH levels thereby modulating heart contractility. Therefore it is essential for heart cells to maintain Na+ homeostasis. Among the proteins that accomplish this task are the Na+/Ca2+ exchanger (NCX) and the Na+/K+ pump (NKA). By transporting three Na+ ions into the cytoplasm in exchange for one Ca2+ moved out, NCX is one of the main Na+ influx mechanisms in cardiomyocytes. Acting in the opposite direction, NKA moves Na+ ions from the cytoplasm to the extracellular space against their gradient by utilizing the energy released from ATP hydrolysis. A fine balance between these two processes controls the net amount of intracellular Na+ and aberrations in either of these two systems can have a large impact on cardiac contractility. Due to the relevant role of these two proteins in Na+ homeostasis, the emphasis of this review is on recent developments regarding the cardiac Na+/Ca2+ exchanger (NCX1) and Na+/K+ pump and the controversies that still persist in the field. PMID:25772291

Posterior parietal cortex role in a sensorimotor task performance Papel do córtex parietal posterior na realização de uma tarefa sensório-motora

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Sergio Nader

2008-06-01

Full Text Available This study aimed to elucidate electrophysiological and cortical mechanisms involved in anticipatory actions when individuals had to catch balls in free drop; specifically through quantitative electroencephalography (qEEG alpha absolute power changes. The sample was composed for 23 health subjects, both sexes, with ages varying between 25 and 40 years, absence of mental and physical illness, right handed and don't make use of any psychoactive or psychotropic substance at the time of the study. The experiment consisted of a task of catching balls in free drop. The three-way ANOVA analysis demonstrated an interaction between moment and position factors in left parietal posterior cortex (PPC (p=0.001. Through the experimental task employed, this area demonstrated a differentiated activity involving expectation, planning and preparedness in the ball's drop task.O estudo tentou elucidar mecanismos eletrofisiológicos e corticais envolvidos em ações antecipatórias quando os sujeitos testados tiveram que apreender bolas em queda livre; especificamente através de mudanças na potência absoluta na banda alfa da eletrencefalografia quantitativa (EEGq. A amostra foi composta por 23 sujeitos sadios, ambos os sexos, idade entre variando entre 25 e 40 anos, sem comprometimento físico e mental, destros, e não fazer uso de nenhuma substância psicoativa ou psicotrópicos até o momento do estudo. O experimento consistiu de uma tarefa de apreensão de bolas em queda livre. A análise three-way ANOVA demonstrou uma interação entre os fatores momento e posição no córtex parietal posterior (CPP esquerdo (p=0,001. Através da tarefa experimental empregada, esta área demonstrou uma atividade diferenciada envolvendo expectativa, planejamento e prontidão na tarefa de queda de bolas.

Efeito do treinamento físico como modulador positivo nas alterações no eixo neuroimunoendócrino em indivíduos com insuficiência cardíaca crônica: possível atuação do fator de necrose tumoral-alfa Efecto del entrenamiento físico como modulador positivo en las alteraciones en el eje neuroinmunoendócrino en indivíduos con insuficiência cardíaca crônica: posible actuación como factor de necrosis tumoral-alfa Effect of physical training as positive modulator on the alterations in the neuro-immune-endocrine axis in patients with chronic heart failure: possible role of the tumoral necrosis factor-alpha

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Luís Fernando Bicudo Pereira Costa Rosa

2005-08-01

Full Text Available O exercício físico crônico ou o treinamento físico (TF tem sido largamente utilizado nos últimos anos com finalidade terapêutica e preventiva em uma série de condições fisiopatológicas, incluindo doenças cardiovasculares. Além dos benefícios cardiovasculares, o TF modula a expressão elevada de citocinas pró-inflamatórias, atuando sobre o eixo neuroimunoendócrino. Atualmente, o quadro de insuficiência cardíaca crônica (ICC tem sido reconsiderado como uma interação entre mecanismos hemodinâmicos, neurormonais, endócrinos e imunológicos. Esta resposta inflamatória anormal, incluindo a elevada expressão de citocinas pró-inflamatórias, tem sido proposta como responsável pela progressão e deterioração clínica na ICC. Neste quadro, a principal citocina envolvida no quadro fisiopatológico da ICC é o fator de necrose tumoral-alfa (TNF-alfa. Assim, o TF pode atuar no quadro de ICC de duas maneiras, melhorando o desempenho durante o exercício físico, bem como atenuando do quadro deletério de elevada concentração de citocinas pró-inflamatórias no sistema cardiovascular, podendo representar importante opção imunomodulatória e, desta forma, permitir melhora significativa no quadro clínico do paciente.El ejercicio físico o entrenamiento físico (TF viene siendo utilizado largamente en los ultimos años con finalidad terapéutica y preventiva en una serie de condiciones fisiopatológicas, incluyendo las afectaciones cardiovasculares. Además de los beneficios cardiovasculares, el TF modula la expresión elevada de las citocinas pro-inflamatorias, actuando sobre el eje neuroinmunoendócrino. Actualmente, el cuadro de insuficiencia cardíaca crônica (ICC viene siendo reconsiderado como una interación entre los mecanismos hemodinámicos, neuro-hormonales, endócrinos e imunológicos. Esta respuesta inflamatoria anormal, incluyendo la elevada expresión de citoquinas pro-inflamatorias, viene siendo propuesta como

Composicao quimica, perfil de acidos graxos e quantificacao dos acidos ƒ¿-linolenico, eicosapentaenoico e docosahexaenoico em visceras de tilapias (Oreochromis niloticus = Percentual composition, fatty acids and quantification of the LNA (Alfa-Linolenic, EPA (Eicosapentaenoic and DHA (Docosahexaenoic acids in visceras of Nile Tilapia (Oreochromis niloticus

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Nilson Evelázio de Souza

2005-01-01

Full Text Available Foi avaliada a composição química de vísceras de tilápias (Oreochromis niloticus criadas em cativeiro Os teores de umidade, cinza, proteína bruta e lipídios totais foram de 64,4%; 1,3%; 6,3% e 18,0%, respectivamente, caracterizando alta concentração de lipídiostotais em relação a outros resíduos de peixes. Foram identificados 49 ácidos graxos, sendo majoritários os ácidos: oléico, (32,8%, seguido do palmítico, (19,9% e linoléico, (18,2%. As razões entre n-6/n-3 e ácidos poliinsaturados/saturados foram de 5,5 e 0,9, respectivamente. As quantificações dos ácidos graxos alfa-linolênico, eicosapentaenóico e docosahexaenóico, em mg/g de lipídios totais, foram de 10,4, 1,4 e 9,3, respectivamente. O elevado teor de lipídios totais das vísceras contribuiu significativamente para as quantidadesde ácidos graxos n-3. Todos os parâmetros analisados foram satisfatórios sob o ponto de vista nutricional e neste sentido as vísceras de tilápias poderão ser utilizadaa para alimentar peixes ou outros animais.The chemical composition was evaluated in visceras of tilapias raised in captivity. The moisture, ash, crude protein and total lipids contents were 64.4%; 1.3%; 6.3% and 18.0%, respectively, characterizing high total lipids concentration in relation other residues of fish. Forty nine fatty acids were detected, the major fatty acids were oleic (32.8%, palmitic (19.9% and linoleic-1 (18.2% and oleic (9.4%. The ratio n-6/n-3 and polyunsaturated/saturated fatty acids, showed the values 5.5 and 0.9, respectively. The quantifications of alfa-linolenic, eicosapentaenoic and docosahexaenoic acids (in mg/g of total lipids, were 10.4, 1.4 and 0.3, respectively. The higher contents of total lipids in visceras contributed significantly for amounts of n-3 fatty acids. All the parameters analyzed were shown nutritional value satisfactory in this sense visceras of tilapias can be used in the feed of fish and other animal.

Frontopolar and anterior temporal cortex activation in a moral judgment task: preliminary functional MRI results in normal subjects Ativação do córtex frontopolar e temporal anterior em uma tarefa de julgamento moral: resultados preliminares de ressonância magnética funcional em indivíduos normais

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Jorge Moll

2001-09-01

ário", enquanto a outra metade era constituída de afirmativas factuais desprovidas de conotação moral ("Pedras são feitas de água". Depois do estudo funcional, cada indivíduo aferiu o conteúdo moral, a valência emocional, e a dificuldade de julgamento de cada sentença em escalas de Likert. Para excluir o efeito da emoção nos resultados da ativação, as respostas individuais foram hemodinamicamente modeladas para análise de RMf relacionada a eventos. O modelo linear geral foi empregado na avaliação das áreas cerebrais ativadas pelos julgamentos morais. RESULTADOS: As regiões ativadas pelo julgamento moral compreenderam o córtex frontopolar, o giro frontal medial, o córtex temporal anterior direito, o núcleo lenticular, e o cerebelo. As ativações frontopolar e médio-frontal (áreas 10/46 e 9 de Brodmann mostraram-se independentes da experiência emocional e representaram as maiores áreas de ativação. CONCLUSÃO: Estes resultados vão ao encontro de observações clínicas que atribuem papel crítico aos pólos frontais e ao córtex temporal anterior direito na regulação do comportamento social. O sistema frontopolar-ântero-temporal descrito no presente trabalho pode representar sistema neural relativamente independente, que opera em harmonia com os córtices orbitário e dorsolateral durante decisões baseadas em julgamentos morais.

ÍNDICE DA QUALIDADE DO SONO DE PITTSBURGH PARA USO NA REABILITAÇÃO CARDIOPULMONAR E METABÓLICA

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Pablo Antonio Bertasso de Araujo

2015-12-01

Full Text Available Introdução A qualidade do sono constitui-se em parâmetro relevante na avaliação da saúde em geral, sendo um fator relevante na determinação do risco das doenças cardiovasculares. Objetivo Validar a versão adaptada do questionário de avaliação do Índice de Qualidade do Sono de Pittsburgh (PSQI para uso em programas de reabilitação cardiopulmonar e metabólica (RCPM. Métodos Estudo descritivo transversal realizado com 101 pacientes de ambos os sexos, com média de idade de 66,05 (± 13,9 anos. Para a análise estatística foi considerado intervalo de confiança de 95% e valor de significância p <0,05; para a análise de consistência interna foi utilizado o coeficiente de alfa de Cronbach e para a análise da relação entre componentes e itens com o escore total do questionário foi utilizado o coeficiente de correlação de Spearman. Resultados Todos os componentes do questionário apresentaram boa consistência interna com valor de 0,72. Os componentes que mais se relacionaram com o escore total foram "duração do sono" e "qualidade subjetiva do sono", sendo que o componente que menos se relacionou foi "alterações do sono". Dentre os itens a variação foi de 0,584 no item "durante a última semana, em geral, como você classificaria a qualidade do seu sono?", até -0,611 no item "durante a última semana, quantas horas você conseguia dormir durante a noite?". Foi possível observar que os itens "tossir ou roncar muito alto" e "frequência para dificuldades do sono por outras razões" não apresentaram correlação com o escore total do questionário. Conclusão A versão adaptada do PSQI mostrou-se válida para ser utilizada na avaliação do sono em programas de RCPM.

Estudo da ativação ácida e tratamento térmico de bauxita extraída de jazidas em Minas Gerais, Brasil Study of acid activation and thermal treatment of bauxite extracted from deposits in Minas Gerais, Brazil

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C. M. R. Prado

2012-03-01

Full Text Available O Brasil é um dos maiores produtores mundiais de bauxita, contudo pouco se conhece sobre as características químicas e mineralógicas desse mineral. Assim sendo, este trabalho tem por objetivo dar prosseguimento à caracterização de bauxita, estudando as transformações ocorridas nesse material quando submetido à ativação ácida e ao tratamento térmico. Os resultados demonstraram que a bauxita "in natura" é composta basicamente pelo mineral gibbsita, seguido de semicristalitos de goethita e óxido de silício, apresentando característica de sólido com ausência de mesoporosidade, com baixos valores de área superficial total, diâmetro e volume de poros. As modificações químicas e físicas que ocorrem no material durante a calcinação são governadas, principalmente, pela desidratação das fases de hidróxidos de alumínio presentes, levando à formação das fases boehmita, hematita e alfa alumina, além de resultar em aumento na porosidade e na área superficial da bauxita. As bauxitas ativadas em meio ácido mostraram que as transformações sofridas no sólido estão diretamente relacionadas com a concentração da solução ácida utilizada e resultam em aumento da área superficial total, diâmetro e volume de poros.Brazil is one of the world's leading producers of bauxite. Little is known, however, about the chemical and mineralogical characteristics of Brazilian bauxite. The objective of this paper is to characterize bauxite in both natural and thermally and chemically activated forms. The transformations occurring during these two processes are described. The results show that the raw bauxite is basically composed of the mineral gibbsite, followed by semicrystalites of goethite and silicon oxide. Its characteristics are those of a solid without mesoporosity, with small total surface area and pore diameter and volume. The chemical and physical modifications that occur in the material during the calcination process are

Altered Na+ transport after an intracellular alpha-subunit deletion reveals strict external sequential release of Na+ from the Na/K pump.

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Yaragatupalli, Siddhartha; Olivera, J Fernando; Gatto, Craig; Artigas, Pablo

2009-09-08

The Na/K pump actively exports 3 Na(+) in exchange for 2 K(+) across the plasmalemma of animal cells. As in other P-type ATPases, pump function is more effective when the relative affinity for transported ions is altered as the ion binding sites alternate between opposite sides of the membrane. Deletion of the five C-terminal residues from the alpha-subunit diminishes internal Na(+) (Na(i)(+)) affinity approximately 25-fold [Morth et al. (2007) Nature 450:1043-1049]. Because external Na(+) (Na(o)(+)) binding is voltage-dependent, we studied the reactions involving this process by using two-electrode and inside-out patch voltage clamp in normal and truncated (DeltaKESYY) Xenopus-alpha1 pumps expressed in oocytes. We observed that DeltaKESYY (i) decreased both Na(o)(+) and Na(i)(+) apparent affinities in the absence of K(o)(+), and (ii) did not affect apparent Na(o)(+) affinity at high K(o)(+). These results support a model of strict sequential external release of Na(+) ions, where the Na(+)-exclusive site releases Na(+) before the sites shared with K(+) and the DeltaKESYY deletion only reduces Na(o)(+) affinity at the shared sites. Moreover, at nonsaturating K(o)(+), DeltaKESYY induced an inward flow of Na(+) through Na/K pumps at negative potentials. Guanidinium(+) can also permeate truncated pumps, whereas N-methyl-D-glucamine cannot. Because guanidinium(o)(+) can also traverse normal Na/K pumps in the absence of both Na(o)(+) and K(o)(+) and can also inhibit Na/K pump currents in a Na(+)-like voltage-dependent manner, we conclude that the normal pathway transited by the first externally released Na(+) is large enough to accommodate guanidinium(+).

Análisis de consistencia interna mediante Alfa de Cronbach: un programa basado en gráficos dinámicos

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Rubén Ledesma

Full Text Available En este trabajo se presenta una herramienta informática original que permite realizar análisis de consistencia interna (modelo Alfa de Cronbach utilizando métodos gráficos dinámicos. Se trata de un módulo basado en la filosofía del Análisis Exploratorio de Datos y en métodos de visualización estadística, diseñado para asistir al analista en el proceso de construcción de pruebas psicológicas. La herramienta permite analizar la consistencia interna de la prueba, las propiedades de los ítems que la componen, los patrones de respuesta de los sujetos a los ítems, y el efecto de la eliminación de los ítems y del incremento en la longitud de la prueba sobre su fiabilidad. En comparación con otros programas existentes, el beneficio del módulo es la incorporación de gráficos estadísticos dinámicos como complemento a la presentación de resultados convencionales en formato texto.

Active transport of Na+ by reconstituted Na,K-ATPase

International Nuclear Information System (INIS)

Boldyrev, A.A.; Svinukhova, I.A.

1987-01-01

The ability of ATP, CTP, ITP, GTP, and UTP to support ouabain-sensitive accumulation of Na + by proteoliposomes with a reconstituted Na/K-pump was investigated. At a low [Na + ]/[K + ] ratio in the medium (20 mM/50 mM), a correlation is observed between the proton-accepting capacity of the nucleotide and its effectiveness as a substrate of active transport. To test the hypothesis of the importance of the presence of a negative charge in the 1-position of the purine (3-pyrimidine) base of the nucleotide for mutual transitions between the Na- and K-conformations of Na,K-ATPase they used two analogs of ATP: N 1 -hydroxy-ATP, possessing proton acceptor capacity, and N 1 -methoxy-ATP, in the molecule of which the negative charge is quenched by a methyl group. The first substrate supports active accumulation of Na + in proteoliposomes at the same rate as ATP, whereas the second substrate is relatively ineffective

Investigation on U - O - Na, Pu - O - Na and U,Pu - O - Na phase diagrams

International Nuclear Information System (INIS)

Pillon, S.

1989-03-01

The thermochemical interaction between the nuclear fuel (uranium and plutonium mixed oxides) and the sodium has been investigated and particularly the three phase diagrams: U - O - Na; Pu - O - Na; U,Pu - O - Na. High temperature neutron diffraction, microcalorimetry and powder X-ray diffraction were used for the characterization of the compounds synthetized. This study allowed to complete the knowledge about each of these diagrams and to measure some physical and thermal properties on the compounds. The limits on the modelization of the fuel-sodium interaction are discussed from the results of the UO 2 - Na reaction [fr

Generalized anxiety disorder and the Hamilton Anxiety Rating Scale in Parkinson's disease Transtorno de ansiedade generalizada e a Escala de Ansiedade de Hamilton na doença de Parkinson

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Arthur Kummer

2010-08-01

Full Text Available Anxiety is common in Parkinson's disease (PD, but studies concerning specific anxiety disorders are scarce. Essential psychometric properties of anxiety rating scales are also lacking. OBJECTIVE: To investigate general anxiety disorder (GAD in PD and psychometric properties of the Hamilton Anxiety Rating Scale (Ham-A. METHOD: Ninety-one PD patients underwent neurological and psychiatric examination, which included the MINI-Plus, the Ham-A and the Hamilton Depression Rating Scale (Ham-D. RESULTS: GAD was present in 30.8% of PD patients. Patients with GAD had longer disease duration (p=0.044 and were in use of higher doses of levodopa (p=0.034. They also tended to have more motor fluctuations and dyskinesias. The group with GAD scored higher in Ham-A (pAnsiedade é comum na doença de Parkinson (DP, mas estudos sobre transtornos de ansiedade específicos são ainda escassos. Faltam também estudos sobre propriedades psicométricas essenciais das escalas de ansiedade. OBJETIVO: Investigar o transtorno de ansiedade generalizada (TAG na DP e propriedades psicométricas da Escala de Ansiedade de Hamilton (Ham-A. MÉTODO: Noventa e um pacientes com DP se submeteram a exames neurológico e psiquiátrico, que incluiu o MINI-Plus, a Ham-A e a Escala de Depressão de Hamilton (Ham-D. RESULTADOS: TAG esteve presente em 30,8% dos participantes. Pacientes com TAG tinham maior duração de doença (p=0,044 e estavam em uso de maiores doses de levodopa (p=0,034. Também havia uma tendência desses pacientes terem mais flutuações motoras e discinesias. O grupo com TAG pontuou mais alto na Ham-A (p<0,001, nas subescalas somática (p<0,001 e psíquica da Ham-A (p<0,001, e na Ham-D (p=0,004. A Ham-A mostrou boa consistência interna (alfa de Cronbach=0,893 e um ponto de corte de 10/11 é sugerido para triar o TAG. CONCLUSÃO: TAG é freqüente na DP e a Ham-A pode ser um instrumento útil para triar esse transtorno.

Limb-girdle muscular dystrophy: an immunohistochemical diagnostic approach Distrofias musculares de cinturas: uma abordagem diagnóstica imuno-histoquímica

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Enio Alberto Comerlato

2005-06-01

Full Text Available The limb-girdle muscle dystrophy (LGMD represents a heterogeneous group of muscular diseases with dominant and recessive inheritance, individualized by gene mutation. A group of 56 patients, 32 males and 24 females, with suggestive LGMD diagnosis were submitted to clinical evaluation, serum muscle enzymes, electromyography, muscle biopsy, and the immunoidentification (ID of sarcoglycans (SG alpha, beta, gamma and delta, dysferlin and western blot for calpain-3. All the patients had normal ID for dystrophin (rod domain, carboxyl and amine terminal. The alpha-SG was normal in 42 patients, beta-SG in 28, beta-SG in 45, delta-SG in 32, dysferlin in 37 and calpain-3 in 9. There was a reduction in the alpha-SG in 7 patients, beta-SG in 4, gamma-SG in 2, and delta-SG in 8. There was deficiency of alpha-SG in 7 patients, beta-SG in 6, gamma-SG in 9, delta-SG in 5, dysferlin in 8, and calpain-3 in 5. The patients were grouped according the ID as sarcoglycans deficiency 18 cases, dysferlin deficiency 8 cases and calpain-3 deficiency 5 cases. Only the sarcoglycans deficiency group showed calf hypertrophy. The dysferlin deficiency group was more frequent in females and the onset was later than sarcoglycan and calpain-3 deficiency groups. The calpain-3 deficiency group occurred only in males and showed an earlier onset and weaker muscular strength.As distrofias musculares de cinturas (DMC representam grupo heterogêneo de doenças musculares com heranças autossômicas dominante ou recessivas, caracterizadas geneticamente por mutações gênicas específicas. Cinqüenta e seis pacientes, 32 masculinos e 24 femininos, com diagnóstico sugestivo de DMC, foram submetidos a avaliação clínica, dosagem séricas das enzimas musculares, eletromiografia, biópsia muscular e imunoidentificação (ID das proteínas sarcoglicanas (SG alfa, beta, gama e delta, disferlina e calpaína-3. A ID da distrofina (domínio rod e terminais carboxila e amino era normal em todos

Measurement of exchangeable sodium: 22Na or 24Na

International Nuclear Information System (INIS)

Smith, T.; Edmonds, C.J.

1987-01-01

A case is made for the use of 22 Na in low activities in preference to 24 Na for routine diagnostic estimation of exchangeable sodium, and is based chiefly on considerations of availability, cost and radiation dosimetry. A method in which only 37 kBq (1 μCi) 22 Na is administered orally is shown to be sufficiently accurate and to possess distinct advantages in terms of cost and convenience with a committed radiation dose no greater than that for a measurement using 24 Na. (author)

Suppression of Na interstitials in Na-F codoped ZnO

Science.gov (United States)

Huo, Wenxing; Mei, Zengxia; Tang, Aihua; Liang, Huili; Du, Xiaolong

2018-04-01

Controlling the formation of interstitial Na (Nai) self-compensating defects has been a long-term physics problem for effective Na doping in ZnO. Herein, we present an experimental approach to the suppression of Nai defects in ZnO via Na and F codoping under an oxygen-rich condition during the molecular beam epitaxy growth process. It is found that the incorporation of such large numbers of Na and F dopants (˜1020 cm-3) does not cause an obvious influence on the lattice parameters. Hall-effect measurements demonstrate that F doping efficiently raises the Fermi level (EF) of ZnO films, which is expected to make the formation energy of Nai and NaZn increase and decrease, respectively. Most of the Na atoms occupy the substitutional Zn sites, and the formation of Nai is suppressed consequently. Secondary ion mass spectrometry measurements reveal that F and Na atoms are tightly bonded together due to their strong Coulomb interaction. The enhanced deep level emission (DLE) in ZnO:Na-F is ascribed to the considerable amount of isolated Zn vacancy (VZn) defects induced by the elevated EF and the formation of neutral (" separators="| FO + - Na Zn - ) 0 complexes. On the other hand, formation of (" separators="| FO + - VZn 2 - ) - complexes in ZnO:F exhausts most of the isolated Zn vacancies, leading to the disappearance of the DLE band.

Estudo morfológico do efeito radioprotetor da vitamina E (dl-alfa-tocoferil na reparação tecidual em ratos

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Manzi Flávio Ricardo

2003-01-01

Full Text Available Esta pesquisa teve por finalidade avaliar a ação da vitamina E como radioprotetora no processo de reparação tecidual em ratos, após sofrerem um procedimento cirúrgico, que consistiu da produção de uma ferida na região dorsal anterior. Os animais foram divididos em cinco grupos: grupo CO (controle - constituído de animais em que foi produzida somente a ferida; grupo VE - pré-tratamento com vitamina E (90 UI; grupo IR - irradiação três dias após a cirurgia; grupo VEIR - pré-tratamento com 90 UI de vitamina E e irradiação de suas bordas três dias após a cirurgia; grupo OIR - pré-tratamento com óleo de oliva e irradiação de suas bordas três dias após a cirurgia. A ação radioprotetora da vitamina E foi avaliada pela coloração por hematoxilina-eosina para análise morfológica do tecido de granulação, aos 4, 7, 14 e 21 dias após a cirurgia. A análise dos resultados mostrou que o retardo no processo de reparação tecidual causado por 6 Gy de radiação de elétrons com feixe de 6 MeV não ocorreu no grupo de animais que recebeu vitamina E, mostrando-se esta substância efetiva como radioprotetora.

Seguridad de la terapia de interferón alfa 2b recombinante más ribavirina en la hepatitis crónica C Safety of recombinant interferon alpha 2b plus ribavirin in chronic hepatitis C

OpenAIRE

Yoan Antonio Sánchez Rodríguez; Enrique Arús Soler; Pedro López Saura; Hugo Nodarse Cuní

2011-01-01

INTRODUCCIÓN: la hepatitis crónica C ha adquirido rango de pandemia. El virus de la hepatitis C se ha convertido en la causa principal de hepatitis crónica, cirrosis hepática, hepatocarcinoma, y trasplante de hígado a nivel mundial. OBJETIVO: identificar los efectos adversos asociados a la terapia combinada interferón alfa 2b recombinante más ribavirina durante la evolución del tratamiento y hasta 8 semanas después de finalizado, así como los principales efectos asociados a salidas temporales...

OGLAŠEVANJE NA DRUŽBENIH OMREŽJIH NA PRIMERU FACEBOOKA

OpenAIRE

Novak, Martina

2012-01-01

V diplomskem delu obravnavamo družbene medije kot novo priložnost za promocijo in oglaševanje podjetij. Spoznali smo družbena omrežja na splošno in nekaj teh, ki jih uporabljamo tudi v Sloveniji na kratko predstavili. Podrobneje smo se osredotočili na družbeno omrežje Facebook, predvsem na samo spletno stran, njen izgled in storitve, ki jih ponuja uporabnikom. V nalogi je podrobneje opisan Facebook kot orodje za uporabo oglaševanja. Spoznali smo brezplačne in plačljive načine promocije in ogl...

Comparison of corifollitropin alfa and daily recombinant follicle-stimulating hormone in poor responder patients undergoing in vitro fertilization cycles

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Süleyman Akarsu

2017-12-01

Full Text Available Objective The aim of this study was to compare the effect of corifollitropin alfa (CFA and recombinant follicle-stimulating hormone (rFSH in poor-responder patients undergoing antagonist cycles. Materials and Methods The study was a retrospective analysis of the treatment results of 214 poor responder patients who had been admitted to the In Vitro Fertilization Unit of İzmir Medical Park Hospital between November 2014 and November 2016. Intracytoplasmic sperm injections were performed in 38 patients (group 1 with CFA, and the remaining 176 (group 2 with rFSH for controlled ovarian hyperstimulation. Results The age, body mass index, anti-müllerian hormone level, duration of infertility, duration of induction and antral follicle number were similar in the two groups. There was no difference in the total aspirated oocyte counts, mature oocyte ratio, fertilization rate, implantation rate, and clinical pregnancy rates between the two groups. The implantation rate was 9/38 (23.6% in group 1 and 42/176 (23.8% in group 2, whereas the clinical pregnancy rates were 16.3% and 17.2%, respectively. Conclusion No difference was found in terms of oocyte count, fertilization rate, implantation rate, and clinical pregnancy rates of CFA or rFSH use in the antagonist cycles in poor-responder patients.

Na+,K+-ATPase Na+ affinity in rat skeletal muscle fiber types

DEFF Research Database (Denmark)

Kristensen, Michael; Juel, Carsten

2010-01-01

Previous studies in expression systems have found different ion activation of the Na(+)/K(+)-ATPase isozymes, which suggest that different muscles have different ion affinities. The rate of ATP hydrolysis was used to quantify Na(+),K(+)-ATPase activity, and the Na(+) affinity of Na(+),K(+)-ATPase...

Molecular basis of mammalian cell invasion by Trypanosoma cruzi

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Nobuko Yoshida

2006-03-01

Full Text Available Establishment of infection by Trypanosoma cruzi, the agent of Chagas' disease, depends on a series of events involving interactions of diverse parasite molecules with host components. Here we focus on the mechanisms of target cell invasion by metacyclic trypomastigotes (MT and mammalian tissue culture trypomastigotes (TCT. During MT or TCT internalization, signal transduction pathways are activated both in the parasite and the target cell, leading to Ca2+ mobilization. For cell adhesion, MT engage surface glycoproteins, such as gp82 and gp35/50, which are Ca2+ signal-inducing molecules. In T. cruzi isolates that enter host cells in gp82-mediated manner, parasite protein tyrosine kinase as well as phospholipase C are activated, and Ca2+ is released from I P3-sensitive stores, whereas in T. cruzi isolates that attach to target cells mainly through gp35/50, the signaling pathway involving adenylate cyclase appears to be stimulated, with Ca2+ release from acidocalciosomes. In addition, T. cruzi isolate-dependent inhibitory signals, mediated by MT-specific gp90, may be triggered both in the host cell and the parasite. The repertoire of TCT molecules implicated in cell invasion includes surface glycoproteins of gp85 family, with members containing binding sites for laminin and cytokeratin 18, enzymes such as cruzipain, trans-sialidase, and an oligopeptidase B that generates a Ca2+-agonist from a precursor molecule.O estabelecimento da infecção por Trypanosoma cruzi, o agente da doença de Chagas, depende de uma série de eventos envolvendo interações de diversas moléculas do parasita com componentes do hospedeiro. Focalizamos aqui os mecanismos de invasão celular por tripomastigotas metacíclicos (TM e por tripomastigotas de cultura de tecido (TCT. Durante a internalização de TM ou TCT, vias de transdução de sinal são ativadas tanto no parasita como na célula alvo, acarretando a mobilização de Ca2+. Para adesão, TM utiliza as glicoprote

Fisiomotricidade e limiares de dor: efeitos de um programa de exercícios na autonomia funcional de idosas osteoporóticas

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Karla Virgínia Bezerra de Castro

Full Text Available INTRODUÇÃO: A atividade física é importante para a manutenção da autonomia funcional do homem e crucial em idosos. Todavia, alguns fatores, como a dor, limitam a participação deles em programas de atividade física. OBJETIVO: Investigar se, em função de um programa de atividades físicas estruturado de maneira a limitar a dor, poder-se-ia garantir a aderência de idosas sofrendo de osteoporose/osteopenia e, dessa forma, promover melhoras na autonomia funcional destas. DESENVOLVIMENTO: A amostra foi composta por 30 mulheres, entre 65 e 70 anos, divididas em dois grupos definidos em função de um diagnóstico de dor. MATERIAIS E MÉTODOS: Aplicou-se um programa definido pelo termo fisiomotriz, de baixo impacto, caracterizado por lentidão nas mudanças articulares, progredindo em quatro níveis que foram distribuídos em 48 encontros. Os dados da dor e da autonomia funcional, obtidos por meio do teste de dor em escala analógica visual e do protocolo de GDLAM foram estudados por estatística descritiva e inferencial, com o índice ALFA ± 0,05. O instrumento foi uma Análise de Variância de Kruskal Wallis com índice Qui-quadrado (Chi². RESULTADOS: Os resultados revelaram melhora da autonomia funcional e diminuição de dor relatada pelas participantes, fato que consequentemente implementou a aderência destas até ao final do programa. CONCLUSÃO: Conclui-se que o programa fisiomotriz, aplicado em idosas com osteopenia e/ou osteoporose, foi uma forma eficaz na promoção da melhora de sua autonomia funcional, que pode ser atribuída, pelo menos em parte, à diminuição de dor antes relatada pelas idosas.

Crystal structure of Na+, K(+)-ATPase in the Na(+)-bound state.

Science.gov (United States)

Nyblom, Maria; Poulsen, Hanne; Gourdon, Pontus; Reinhard, Linda; Andersson, Magnus; Lindahl, Erik; Fedosova, Natalya; Nissen, Poul

2013-10-04

The Na(+), K(+)-adenosine triphosphatase (ATPase) maintains the electrochemical gradients of Na(+) and K(+) across the plasma membrane--a prerequisite for electrical excitability and secondary transport. Hitherto, structural information has been limited to K(+)-bound or ouabain-blocked forms. We present the crystal structure of a Na(+)-bound Na(+), K(+)-ATPase as determined at 4.3 Å resolution. Compared with the K(+)-bound form, large conformational changes are observed in the α subunit whereas the β and γ subunit structures are maintained. The locations of the three Na(+) sites are indicated with the unique site III at the recently suggested IIIb, as further supported by electrophysiological studies on leak currents. Extracellular release of the third Na(+) from IIIb through IIIa, followed by exchange of Na(+) for K(+) at sites I and II, is suggested.

Clinical Experience of Interferon Alfa-2a Treatment for Refractory Uveitis in Behçet's Disease.

Science.gov (United States)

Park, Ji-Youn; Chung, Yoo-Ri; Lee, Kihwang; Song, Ji Hun; Lee, Eun-So

2015-07-01

Behçet's disease (BD) involves multisystem vasculitis of unknown origin. Ocular manifestations of BD mostly include bilateral panuveitis and retinal vasculitis, which are very challenging to treat. Interferon alfa-2a (IFN) has been recently introduced for treating refractory Behçet uveitis, mainly in Germany and Turkey. Nonetheless, there is so far no consensus about the ideal treatment regimen of IFN for Behçet uveitis. We report our experience of IFN treatment in five Korean BD patients with refractory uveitis. All patients complained of oral ulcers; one patient had a positive pathergy test and 2 showed the presence of HLA-B51. Immunosuppressive agents used prior to IFN treatment included cyclosporine and methotrexate. The IFN treatment was commenced with a dose of 6-9 MIU/day for 7 days, adjusted according to individual ocular manifestations, tapered down to 3 MIU three times in a week, and then discontinued. All patients showed positive response to IFN treatment; 50% of them showed complete response without additional major ocular inflammation during the follow-up period. Other BD symptoms also improved after IFN treatment in most cases. After treatment, the relapse rate and the required dose of oral corticosteroid were decreased in most cases, showing a significant steroid-sparing effect. However, the visual acuity was not improved in most cases due to irreversible macular sequelae. Despite the small sample size of this study, we suggest that, in Korean patients, IFN is an effective treatment modality for BD uveitis as was observed in German and Turkish patients.

AlphaCI: un programa de cálculo de intervalos de confianza para el coeficiente alfa de Cronbach AlphaCI: a computer program for computing confidence intervals around Cronbach's alfa coefficient

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Rubén Ledesma

2004-06-01

Full Text Available El coeficiente alfa de Cronbach es el modo más habitual de estimar la fiabilidad de pruebas basadas en Teoría Clásica de los Test. En dicha estimación, los investigadores usualmente omiten informar intervalos de confianza para el coeficiente, un aspecto no solo recomendado por los especialistas, sino también requerido explícitamente en las normas editoriales de algunas revistas especializadas. Esta situación puede atribuirse a que los métodos de estimación de intervalos de confianza son poco conocidos, además de no estar disponibles al usuario en los programas estadísticos más populares. Así, en este trabajo se presenta un programa desarrollado dentro del sistema estadístico ViSta que permite calcular intervalos de confianza basados en el enfoque clásico y mediante la técnica bootstrap. Se espera promover la inclusión de intervalos de confianza para medidas de fiabilidad, facilitando el acceso a las herramientas necesarias para su aplicación. El programa es gratuito y puede obtenerse enviando un mail de solicitud al autor del trabajo.Cronbach's alpha coefficient is the most popular way of estimating reliability in measurement scales based on Classic Test Theory. When estimating it, researchers usually omit to report confidence intervals of this coefficient, as it is not only recommended by experts, but also required by some journal's guidelines. This situation is because the different methods of estimating confidence intervals are not well-known by researchers, as well as they are not being available among the most popular statistical packages. Therefore, this paper describes a computer program integrated into the ViSta statistical system, which allows computing confidence intervals based on the classical approach and using bootstrap technique. It is hoped that this work promotes the inclusion of confidence intervals of reliability measures, by increasing the availability of the required computer tools. The program is free and

In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation Efeito in vitro do herbicida glifosato na agregação plaquetária e coagulação sanguínea em humanos

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Teresinha de Jesus C. Neiva

2010-01-01

ágeno 4µg/mL. Pré-tratamento com GP 500µg/mL demonstrou significativa hipofunção dos três agentes agregantes. O efeito inibitório foi dose dependente em concentrações de 50-500 µg/mL. Utilizando-se a quantificação de ATP como um índice da capacidade de secreção plaquetária, foi observado diminuição da liberação das plaquetas tratadas com GP. Por outro lado, o GP não promoveu efeito inibidor no tempo de protrombina (TP, tempo de tromboplastina parcial ativada (ATTP e tempo de trombina (TT. Em conclusão, os resultados demonstram que o GP promove mudanças no metabolismo plaquetário com efeito inibitório na hemostasia primária.

Meltability in system of K2TaF7-NaF-NaCl-KCl

International Nuclear Information System (INIS)

Kartsev, V.E.; Kovalev, F.V.; Korshunov, B.G.

1975-01-01

Thermographic and visual-polythermal techniques were used to study the meltability in K 2 TaF 7 -NaF-NaCl-KCl system. The tetrahedron-forming sections NaF-NaCl-K 2 TaF 7 xKCl and NaF-K 2 TaF 7 xKCl-2K 2 TaF 7 xNaCl divide the concentration tetrahedron into three particular tetrahedra: NaF-K 2 TaF 7 xKCl-2K 2 TaF 7 xNaCl-K 2 TaF 7 , NaF-NaCl-K 2 TaF 7 xKCl-2K 2 TaF 7 xaCl, and NaF-NaCl-KCl-K 2 TaF 7 xKCl. Non-variant equilibrium points in all of the particular four-component systems have been determined

Dgroup: DG01750 [KEGG MEDICUS

Lifescience Database Archive (English)

Full Text Available tical recombination) (JAN) D02745 ... Interferon alfa-2b (USAN); Interferon alfa-2b (genetica... DG01750 Chemical ... DGroup Interferon alpha ... D00745 ... Interferon alfa-2a (USAN/INN); Interferon alfa-2a (gene

Active transport of Na/sup +/ by reconstituted Na,K-ATPase

Energy Technology Data Exchange (ETDEWEB)

Boldyrev, A.A.; Svinukhova, I.A.

1987-02-20

The ability of ATP, CTP, ITP, GTP, and UTP to support ouabain-sensitive accumulation of Na/sup +/ by proteoliposomes with a reconstituted Na/K-pump was investigated. At a low (Na/sup +/)/(K/sup +/) ratio in the medium (20 mM/50 mM), a correlation is observed between the proton-accepting capacity of the nucleotide and its effectiveness as a substrate of active transport. To test the hypothesis of the importance of the presence of a negative charge in the 1-position of the purine (3-pyrimidine) base of the nucleotide for mutual transitions between the Na- and K-conformations of Na,K-ATPase they used two analogs of ATP: N/sub 1/-hydroxy-ATP, possessing proton acceptor capacity, and N/sub 1/-methoxy-ATP, in the molecule of which the negative charge is quenched by a methyl group. The first substrate supports active accumulation of Na/sup +/ in proteoliposomes at the same rate as ATP, whereas the second substrate is relatively ineffective.

Relationship between intracellular Na+ concentration and reduced Na+ affinity in Na+,K+-ATPase mutants causing neurological disease

DEFF Research Database (Denmark)

Toustrup-Jensen, Mads Schak; Einholm, Anja P.; Schack, Vivien

The neurological disorders familial hemiplegic migraine type 2 (FHM2), alternating hemiplegia of childhood (AHC), and rapid-onset dystonia parkinsonism (RDP) are caused by mutations of Na+,K+-ATPase α2 and α3 isoforms, expressed in glial and neuronal cells, respectively. Although these disorders......, addressing the question to what extent they cause a change of the intracellular Na+ and K+ concentrations ([Na+]i and [K+]i) in COS cells. C-terminal extension mutants generally showed dramatically reduced Na+ affinity without disturbance of K+ binding, as did other RDP mutants. No phosphorylation from ATP...

MOŽNOSTI VSTOPA NA TUJE TRGE NA PRIMERU PODJETJA AWOODTURE

OpenAIRE

Orličnik, Urša

2014-01-01

Danes pojavljanje podjetja samo na domačem trgu ni več dovolj. Spremembe okolja, naraščanje konkurence ter hiter tehnološki napredek, silijo podjetja k nenehnemu prilagajanju in iskanju novih priložnosti tako na domačem kot tudi na tujem trgu. Internacionalizacija podjetja tako postaja vedno bolj pomemembna za preživetje podjetja. Internacionalizacija se v najširšem smislu nanaša na vse oblike mednarodnega ekonomskega sodelovanja. Je dinamičen proces, kateremu nujno sledi sprememba stanja...

Tratamiento de Artritis reumatoide con antagonistas del factor de necrosis tumoral alfa y su asociación con el desarrollo de melanoma cutáneo: revisión sistemática de la literatura y meta-análisis.

OpenAIRE

Aldana Silva, Carolina; Chaparro Reyes, Laura Daniela

2015-01-01

Introducción: El tratamiento con antagonistas del factor de necrosis tumoral alfa (anti TNF) ha impactado el pronóstico y la calidad de vida de los pacientes con artritis reumatoide (AR) positivamente, sin embargo, se interroga un incremento en el riesgo de desarrollar melanoma. Objetivo: Conocer la asociación entre el uso de anti TNF y el desarrollo de melanoma maligno en pacientes con AR. Metodología: Se realizó una búsqueda sistemática en MEDLINE, EMBASE, COCHRANE LIBRARY y ...

Na/K pump inactivation, subsarcolemmal Na measurements, and cytoplasmic ion turnover kinetics contradict restricted Na spaces in murine cardiac myocytes

OpenAIRE

Lu, Fang-Min; Hilgemann, Donald W.

2017-01-01

The Na/K pump exports cytoplasmic Na ions while importing K ions, and its activity is thought to be affected by restricted intracellular Na diffusion in cardiac myocytes. Lu and Hilgemann find instead that the pump can enter an inactivated state and that inactivation can be relieved by cytoplasmic Na.

Alterações na distribuição de potência cortical em função da consolidação da memória no aprendizado de datilografia Changes in cortical power distribution produced by memory consolidation as a function of a typewriting skill

Directory of Open Access Journals (Sweden)

Marlo Cunha

2004-09-01

Full Text Available O objetivo do presente estudo foi investigar alterações nos padrões eletroencefalográficos de sujeitos normais e destros durante o aprendizado de uma tarefa motora (datilografia. Estudos têm demonstrado que o córtex cerebral é susceptível a modificações durante a aprendizagem e que alterações nos padrões elétricos corticais ocorrem em função da aquisição de uma habilidade motora e da consolidação da memória. Assim, a atividade elétrica cortical dos sujeitos foi analisada antes e depois da prática motora. Os dados foram captados pelo "Braintech" 3000 e analisados pelo programa "Neurometrics". Para a análise estatística, variáveis comportamentais tais como tempo e erro, foram analisadas através do teste ANOVA "one-way" (diferenças entre blocos. Em seguida, foi utilizado um Teste-t pareado para os pares de eletrodos CZ-C3/CZ-C4, nas bandas de freqüência teta e alfa. Os resultados principais demonstraram mudança na performance através das variáveis comportamentais "tempo" e "número de erros". Concomitantemente, não foram observadas alterações na variável neurofisiológica "Potência Absoluta" na banda teta. Em contrapartida, houve um aumento significativo na banda alfa em áreas centrais (CZ-C3/CZ-C4. Tais resultados sugerem uma adaptação do córtex sensório-motor em função do treinamento de datilografia.The present study aimed to investigate alterations in EEG patterns in normal, right-handed individuals, during the process of learning a specific motor skill (typewriting. Recent studies have shown that the cerebral cortex is susceptible to several changes during a learning process and that alterations in the brain's electrical patterns take place as a result of the acquisition of a motor skill and memory consolidation. In this context, subjects' brain electrical activity was analyzed before and after the motor task. EEG data were collected by a Braintech 3000 and analyzed by Neurometrics. For the

Toxicidade e capacidade de ligação de proteínas Cry1 a receptores intestinais de Helicoverpa armigera (Lepidoptera: Noctuidae

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Isis Sebastião

2015-11-01

Full Text Available Resumo: O objetivo deste trabalho foi avaliar a toxicidade e a capacidade de ligação das proteínas Cry1Aa, Cry1Ab, Cry1Ac e Cry1Ca, de Bacillus thuringiensis, a receptores intestinais de Helicoverpa armigera. Realizou-se análise de ligação das proteínas ativadas às vesículas de membrana da microvilosidade apical (VMMA do intestino médio deH. armigera, além de ensaios de competição heteróloga para avaliar sua capacidade de ligação. Cry1Ac destacou-se como a proteína mais tóxica, seguida por Cry1Ab e Cry1Aa. A proteína Cry1Ca não foi tóxica às lagartas e, portanto, não foi possível determinar os seus parâmetros de toxicidade CL50 e CL90. As proteínas Cry1Aa, Cry1Ab e Cry1Ac são capazes de se ligar a um mesmo receptor nas membranas intestinais, o que aumenta o risco do desenvolvimento de resistência cruzada. Portanto, a utilização conjunta dessas proteínas deve ser evitada.

Terapia Expulsiva Medicamentosa na Litíase Ureteral: Revisão de Literatura/ Medicamental Expulsive Therapy in Ureteral Lithiasis: Literature Review

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Kenji Maeda Missima

2014-06-01

Full Text Available A terapia expulsiva medicamentosa na litíase ureteral tem ganhado importante espaço na prática clínica, visto que é um método não invasivo e de grande eficácia. Como se trata de uma afecção comum, de alta incidência e custo, a terapia expulsiva possibilita um manejo clínico menos dispendioso e invasivo, quando comparada a procedimentos intervencionistas. Há consenso na literatura que a terapia expulsiva medicamentosa é efetiva e deve ser utilizada em cálculos com cerca de 5 milímetros de diâmetro, visto que as drogas utilizadas aumentam a taxa e diminuem o tempo de expulsão, além de diminuir a dor e o número de internações. O tamanho e localização do cálculo são de extrema relevância para que se possa considerar o manejo conservador. As drogas que obtém as melhores taxas expulsivas são os bloqueadores dos receptores alfa-adrenérgicos e os bloqueadores dos canais de cálcio. Analgésicos também são utilizados para o alívio da dor e o uso de corticoides ainda é questionado. Medicamental expulsive therapy for ureteral stones has gained important place in clinical practice, because it is a noninvasive and highly effective method. As it is a common disorder with high incidence and cost, expulsive therapy provides a less costly and invasive clinical management when compared to interventional procedures. There is a consensus that the medicamental expulsive therapy is effective and should be used in calculations with about 5 millimeters in diameter, since the drugs used increase rate and decrease the time of expulsion, in addition to reducing the pain and the number of hospitalizations. The size and location of the calculi are very important for it to be considered conservative management. The drugs that get the best expulsive rates are alpha - adrenergic receptor blockers and calcium channel blockers. Analgesics are also used for pain relief and the use of corticosteroids is still questioned.

Fontes de óleo na dieta e sua influência no desempenho e na imunidade de frangos de corte

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Marcos F. Pinto

2014-05-01

Full Text Available A composição de ácidos graxos da dieta pode influenciar o desempenho produtivo e o sistema imune de frangos de corte. O objetivo deste estudo foi avaliar o efeito do consumo de óleos ricos em ácidos graxos poli-insaturados ômega-6 (PUFAs n-6 e ômega-3 (PUFAs n-3 sobre o desempenho e a resposta imunológica de frangos de corte frente a um desafio antigênico. Foram comparadas dietas formuladas com 7% de óleo de soja (OS, linhaça (OL ou sardinha (OP, fornecidas a 240 frangos da linhagem Cobb, divididos em 24 grupos de 10 aves cada, num arranjo experimental 3x2 (3 tipos de óleo e aves vacinadas ou não vacinadas e 4 repetições. O óleo de soja é rico em ácido linoleico, um PUFA n-6, o óleo de linhaça é fonte de ácido alfa-linolênico, um PUFA n-3, e o óleo de sardinha, de outros PUFAs n-3, como os ácidos eicosapentaenoico e docosahexaenoico. O consumo de ração, o ganho de peso e a conversão alimentar foram avaliados aos 21, 35 e 42 dias. Aos 7 e aos 21 dias de idade, metade das aves recebeu vacina contra doença de Newcastle. Quinze dias após a imunização, avaliou-se a produção de anticorpos pelo método de ELISA, expressa pela densidade óptica a 450 nm (D.O. 450nm. Apenas as aves alimentadas com ração contendo OS apresentaram maior imunidade humoral (P0,05. Entre as aves que receberam dieta com OS, as aves vacinadas apresentaram pior conversão alimentar (P0,05, considerando todo o período experimental. A utilização de óleo rico em PUFA n-6 na dieta de frangos de corte aumentou a resposta humoral, mas não influenciou a resposta celular frente a um desafio antigênico.

Comparative study of sulfated polysaccharides from Caulerpa spp. (Chlorophyceae. Biotechnological tool for species identification?=Estudo comparativo dos polissacarídeos sulfatados de clorofíceas Caulerpa spp. (Chlorophyceae. Ferramenta biotecnológica na identificação de espécies?

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Chistiane Oliveira Coura

2012-10-01

cromatografia de troca iônica em coluna de DEAE-celulose utilizando um gradiente de NaCl. As frações obtidas foram analisadas por eletroforese em gel de agarose a 0,5%. Ensaios anticoagulantes, utilizando o teste do tempo de tromboplastina parcial ativada (TTPA com plasma humano normal e heparina padrão (193 UI mg-1, também foram realizados como parâmetros de comparação. Verificaram-se baixos rendimentos e semelhantes perfis cromatográficos entre os PSs das espécies, porém revelando, por eletroforese, diferenças moleculares marcantes. As alterações no TTPA das frações de PS foram dependentes da densidade de cargas negativas mostradas nos perfis eletroforéticos, cujas atividades foram 17,37 (C. cupressoides, 22,17 (C. racemosa e 25,64 (C. prolifera UI mg-1, respectivamente, e tal propriedade justificou um estudo já realizado utilizando a primeira e segunda espécies. Os resultados sugerem que estudos comparativos de PSs isolados de algas marinhas possam vir a ser uma ferramenta importante na identificação de Caulerpaceae.

Dgroup: DG01752 [KEGG MEDICUS

Lifescience Database Archive (English)

Full Text Available (USAN/INN); Interferon alfa-2a (genetical recombination) (JAN) ... D02745 ... Interferon alfa-2b (USAN); Interferon alfa-2b (genetica... Interferon alfa-n3 (USAN) DG01751 ... Interferon beta ... D00746 ... Interferon beta-1b (USAN/INN); Interferon beta-1b (genetica...D00747 ... Interferon gamma-1b (USAN/INN) ... D03357 ... Interferon gamma-1a (genetical recombination) (JAN) ... D...08805 ... Interferon gamma-n1 (JAN) D02744 ... Interferon alfacon-1 (USAN/INN); Interferon alfacon-1 (genetical re...combination) (JAN) D02747 ... Peginterferon alfa-2a (USAN/INN); Peginterferon alfa-2a (genetica

Validita modelu CAPM na akciovém trhu USA

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Martin Širůček

2014-04-01

Full Text Available Purpose of the article: The present article is focused on the Capital Asset Pricing Model (CAPM and its implementation into American Stock Market. It attempts to empirically test the validity of the CAPM to estimate individual stock returns based on historical stock data of selected companies. Security Market Line (SML was used on the data collected from a wide range of investment horizons (periods of 1, 3, 5, and 10 years. The results show that the coefficient beta is incapable of explaining returns of single assets and the relation between systematic risk and expected return is weak. Methodology/methods: Empirical analysis is make on the time period 2001–2011. Selected stocks has to by trade on AMEX, NASDAQ or NYSE minimal since year 2000. Concrete 10 stock were selected with using branch analysis and were divide in two groups (a cyclic stocks and (b other (neutral-anticyclic stocks. By every stock was watch the closing price which was adjusted of dividends and contain splits. Scientific aim: The aim of this article is by using the model of Security Market Line (SML verify the validity of CAPM model by assets pricing. According to Alfa coefficient is determine how much differentiate is the yield set by CAPM model and the market yield in selected investment horizons. Findings: Selected length of investment horizon has important effect on the results. Worst results were found by the shortest length (1 year. With the growing of the investment horizon are the results better, but the Alfa coefficients is still to higher and the model inaccurate. Selected stocks with lower beta coefficient has higher yield that yield set by the CAPM model. Conclusions: For explanation of the yields by selected stocks we can´t recommend use the CAPM model nor on the biggest stock market, on US stock market. The relation between beta coefficient and stock yield is very weak. But other site CAPM model in the shape of SML curve can be recommended by using as discount

Vpliv toplotnih mostov na porabo energije za ogrevanje na primeru osnovne šole

OpenAIRE

Megušar, Primož

2015-01-01

Na primeru Osnovne šole Dobrova sem preveril, kakšen vpliv imajo dejanski toplotni mostovi na porabo energije za ogrevanje. Iz načrtov stavbe sem evidentiral vse toplotne mostove, kateri vplivajo na porabo energije. Tem toplotnim mostovom sem nato v standardu SIST EN ISO 14683 poiskal ustrezne približke. Nato sem s programom TOST izvedel tri simulacije. V prvem primeru toplotnih mostov nisem upošteval. V drugem primeru sem toplotne mostove upošteval na poenostavljen način, v zadnjem primeru p...

Na and K dependence of the Na/K pump in cystic fibrosis fibroblasts.

OpenAIRE

Reznik, V M; Schneider, J A; Mendoza, S A

1981-01-01

The Na and K dependence of the Na/K pump was measured in skin fibroblasts from patients with cystic fibrosis and age/sex-matched controls. Under basal conditions, there was no difference between control and cystic fibrosis cells in protein per cell, intracellular Na and K content, or Na/K pump activity (measured as ouabain-sensitive 86Rb uptake). There was no difference in the Na dependence of the Na/K pump between cystic fibrosis cells and control cells. In cells from patients with cystic fi...

Vliv mikroflóry na senzorické vlastnosti vína

OpenAIRE

Petrášová, Ludmila

2014-01-01

Tato bakalářská práce se zabývá vlivem mikroflóry na senzorické vlastnosti vína. Teoretická část informuje o botanickém popisu hroznů vína Veltlínské zelené, dále o jeho složení, o technologii zpracování bílého vína a o vlivu kvasinek na aromatický profil vína. V další části popisuje metodu stanovení aromaticky aktivních látek pomocí plynové chromatografie v kombinaci s mikroextrakcí tuhou fází (SPME-GC). Cílem experimentální části bylo proměřit vzorky 4 různých moštů odrůdy Veltlínské zelené...

THE INFLUENCE OF ASOCIATED SUPPLEMENT OF ALFA AMYLASE AND XYLANASE ON THE RHEOLOGY OF DOUGH CONCEARNING ITS CONSTITOGRAPHICAL PARAMETERS

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RODICA CHEREJI

2009-05-01

Full Text Available In this paper we determined the influence of associated supplement of alfa amylase and xylanase on the rheology of dough concearning its constitographical parameters : maximum pressure (Pr max, (mb and the absorbed water (Wa, %. The analysis on the consistograph were conducted for constant hydration at the consistency of 500 UF. Determinations were made on 4 types of flour and optimal dosages were found for each enzyme, after which we prepared the optimal dosage of the enzymes in the compund for flour F1 and F2 : P1-840000 U. SKB/100 kg flour +8100 U. FXU /100 kg flour, P2-840000 U. SKB/100 kg flour+16200 U. FXU, /100 kg flour , P3-840000 U. SKB/100 kg flour+24300 U. FXU/100 kg flour , and for F3 and F4 thus: P1-280000 U. SKB/100 kg flour +8100 U. FXU /100 kg flour, P2- 280000 U. SKB/100 kg flour+16200 U. FXU/100 kg flour, P3-280000 U. SKB/100 kg flour+24300 U. FXU/100 kg flour. Fungous α-amylase and xylanase were used in these concentrations to establish which one is more apropriate to be added in flour to obtain superior quality of bread: finer texture of the crumb, prolongation of the freashness of the bread, improvind the colour and flavour, emproving the slicing ability.

Comparing effects of Beractant and Poractant alfa in decreasing mortality rate due to respiratory distress syndrome in premature infants

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Saeidi R

2011-02-01

Full Text Available "nBackground: Exogenous natural and synthetic surfactants is a rescue treatment for respiratory distress syndrome (RDS. The goals of the study were to compare the clinical response and side-effects of two frequently used surfactants, poractant alfa (Curosurf and beractant (Survanta, for the treatment of respiratory distress syndrome in preterm infants."n "nMethods: This clinical trial study was performed during a two-year period in the Neonatal Intensive Care Unit of Ghaem Hospital in Mashhad, Iran. Sample size calculated by a 95% confidence and power of 80, included 104 premature neonates, 74 in survanta and 30 in curosurf groups. The level of statistical significance was considered to be < 0.05."n "nResults: There were no statistically significant differences between the infants treated by survanta or cursurf groups regarding their mean gestational age (30.58 Vs. 29.00 weeks and birth weight (1388 Vs. 1330 g, (p=0.3 There were also no significant differences between the two groups regarding incidences of broncho- pulmonary dysplasia (BPD (40.5% Vs. 40%, intraventricular hemorrhage (IVH grades III/IV (13.5% Vs. 13.3%, pneumothorax (both 20%, patent ductus arteriosus (PDA (28/3% Vs. 20% or death (28% Vs. 26.6% on the 28th day postpartum."n "nConclusion: This study showed that survanta and curosurf had similar therapeutic effects in the treatment of neonatal respiratory distress syndrome.

Safety and efficacy of REP 2139 and pegylated interferon alfa-2a for treatment-naive patients with chronic hepatitis B virus and hepatitis D virus co-infection (REP 301 and REP 301-LTF): a non-randomised, open-label, phase 2 trial.

Science.gov (United States)

Bazinet, Michel; Pântea, Victor; Cebotarescu, Valentin; Cojuhari, Lilia; Jimbei, Pavlina; Albrecht, Jeffrey; Schmid, Peter; Le Gal, Frédéric; Gordien, Emmanuel; Krawczyk, Adalbert; Mijočević, Hrvoje; Karimzadeh, Hadi; Roggendorf, Michael; Vaillant, Andrew

2017-12-01

REP 2139 clears circulating hepatitis B virus (HBV) surface antigen (HBsAg), enhancing the restoration of functional control of HBV infection by immunotherapy. We assessed the safety and efficacy of REP 2139 and pegylated interferon alfa-2a in patients with chronic HBV and hepatitis D virus (HDV) co-infection. In this open-label, non-randomised, phase 2 trial, patients aged 18-55 years, who were treatment naive, hepatitis B e antigen [HBeAg] negative, anti-hepatitis D antigen [HDAg] positive, and HDV RNA positive, with serum HBsAg concentrations of more than 1000 IU/mL, and a history of HDV infection for 6 months or more before treatment, were recruited at Toma Ciorbă Hospital of Infectious Diseases in Chișinău, Moldova. Patients were excluded if they had HDV superinfection, liver infections other than HBV and HDV, or liver cirrhosis. Patients received 500 mg intravenous REP 2139 once per week for 15 weeks, followed by combined therapy with 250 mg intravenous REP 2139 and 180 μg subcutaneous pegylated interferon alfa-2a once per week for 15 weeks, then monotherapy with 180 μg pegylated interferon alfa-2a once per week for 33 weeks. The primary endpoints assessed at the end of treatment were the safety and tolerability of the treatment regimen, analysed in the intention-to-treat population. Secondary outcomes included the proportion of patients with serum HBsAg less than 50 IU/mL, the proportion of patients with suppressed HBV DNA, and the proportion of patients who maintained these responses through follow-up. The REP 301 trial is registered with ClinicalTrials.gov, number NCT02233075. We also did an additional follow-up at 1 year after the end of treatment, as an interim analysis of the REP 301-LTF trial (planned duration 3 years), registered with ClinicalTrials.gov, number NCT02876419, which is ongoing but not recruiting patients. Between Sept 8, 2014, and Jan 27, 2015, we enrolled 12 patients into the REP 301 study. All 12 patients experienced at least one

Eficácia analgésica da dexmedetomidina comparada ao sufentanil em cirurgias intraperitoneais: estudo comparativo Eficacia analgésica de la dexmedetomidina comparada al sufentanil en cirugías intraperitoneales: estudio comparativo Analgesic efficacy of dexmedetomidine as compared to sufentanil in intraperitoneal surgeries: comparative study

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Marco Aurélio Marangoni

2005-02-01

Full Text Available JUSTIFICATIVA E OBJETIVOS: A dexmedetomidina, agonista alfa2-adrenérgico com especificidade alfa1:alfa2 1:1620, não determina depressão respiratória, sendo utilizada no intra-operatório como sedativo e analgésico. Esse fármaco tem sido empregado com os opióides em anestesia de procedimentos com elevado estímulo doloroso, como os abdominais intraperitoneais, não havendo referências sobre seu uso como analgésico único. Comparou-se a dexmedetomidina ao sufentanil em procedimentos intraperitoneais, de pacientes com mais de 60 anos de idade. MÉTODO: Foram estudados 41 pacientes divididos aleatoriamente em dois grupos: GS (n = 21, que recebeu sufentanil, e GD (n = 20, dexmedetomidina, ambos na indução e manutenção da anestesia. Os pacientes receberam etomidato (GS e GD com midazolam (GD na indução, isoflurano e óxido nitroso na manutenção da anestesia. Foram avaliados os atributos hemodinâmicos (pressão arterial média e freqüência cardíaca, tempos de despertar e de extubação ao final da anestesia, locais onde os pacientes foram extubados - sala de operação (SO ou sala de recuperação pós-anestésica (SRPA, tempo de permanência na SRPA, necessidade de analgesia suplementar e antiemético na SRPA, complicações apresentadas na SO e SRPA, índice de Aldrete-Kroulik na alta da SRPA e a necessidade de máscara de oxigênio na alta da SRPA. RESULTADOS: Não houve diferença quanto à estabilidade hemodinâmica e GD apresentou menor tempo de permanência na SRPA e menor necessidade de máscara de oxigênio na alta da SRPA. CONCLUSÕES: A dexmedetomidina pode ser utilizada como analgésico isolado em operações intraperitoneais em pacientes com mais de 60 anos, determinando estabilidade hemodinâmica semelhante à do sufentanil, com melhores características de recuperação.JUSTIFICATIVA Y OBJETIVOS: La dexmedetomidina, agonista alfa2-adrenérgico con especificidad alfa1:alfa2 1:1620, no determina depresión respiratoria

SureClick® (Darbepoetin alfa) can improve perceived satisfaction and competence for anemia treatment and increase self-administration in non-dialyzed patients with chronic kidney disease.

Science.gov (United States)

Bonafont, Xavier; Romero, Ramón; Martínez, Isabel; del Pino, María D; Gil, José M; Aranda, Pedro; Roca, Ramón; Claverol, Joana; Cucala, Mercedes

2013-01-01

SureClick® is a prefilled pen for administration of darbepoetin alfa (DA) that is ready-to-use. We explored patient satisfaction with SureClick® compared with prefilled syringes (PFS). Multicenter, prospective, 6-months, observational study in non-dialyzed patients with chronic kidney disease (CKD) treated with DA in PFS who switched to SureClick® at baseline. Main outcomes were: change in Anemia Treatment Satisfaction Questionnaire (ATSQ-S), Perceived Competence for Anemia Scale (PCAS) and self-administration rate. We enrolled 132 patients with a mean(SD) age of 71.3 (14.6) years, 57.6% women. Mean(SD) ATSQ-S scores at baseline and final records were 25.5 (7.9) and 31.6 (4.9) (on a scale from 0 to 36 maximum satisfaction-, mean change: 6.2, 95%CI: 4.6-7.8, pscale from 1 to 7 maximum competence, pperceived competence in anemia management in non-dialyzed CKD patients, and could increase the self-administration rate, thereby reducing use of health resources.

Mecanismos moleculares envolvendo a alfa-sinucleína e a sinfilina-1 na doença de Parkinson

OpenAIRE

Tayob, Soraya Barbosa

2014-01-01

Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz A doença de Parkinson (DP) é a patologia neurodegenerativa mais prevalente logo a seguir à doença de Alzheimer, afectando 1% dos indivíduos com idades superiores a 60 anos. A DP é caracterizada, clinicamente, por severos sintomas motores incluindo tremor, rigidez muscular, instabilidade postural e bradicinesia. Uma das principais características é a presença de Corpos de Lewy associada a uma p...

Study of the Na-C-O and Na-H-O ternary systems in the sodium rich corner

International Nuclear Information System (INIS)

Maupre, J.-P.

1978-02-01

The purpose of this study is to provide a contribution to the understanding of the sodium - carbon - oxygen and sodium - hydrogen - oxygen ternary systems in the sodium rich corner. In order to do this the Na-NaH-Na 2 O-NaOH phase diagram was completed and the Na-Na 2 O-Na 2 CO 3 -C phase diagram was outlined. This work is made up of two parts. The first is devoted to a critical literature survey essential to establish correct phase diagrams. The second is an experimental study followed by a discussion collating our finding to the literary data. The basic experimental technique used is differential thermal analysis (DTA) but it has been completed by quenching, X-ray and chemical analysis methods. The proposed phase diagrams imply that Na-NaH-Na 2 O-NaOH and Na-Na 2 O-Na 2 CO 3 -C systems are reciprocal ternary systems. Temperatures of stable pairs reversal are respectively 410 and 690 0 C. The stable pairs are Na-NaOH and Na-Na 2 CO 3 at elevated temperature, Na 2 O-NaH and Na 2 O-C at low temperature [fr

Response of saliva Na/K ratio to changing Na supply of lactating cows under tropical conditions.

Science.gov (United States)

Thiangtum, Wandee; Schonewille, J Thomas; Verstegen, Martin Wa; Arsawakulsudhi, Supot; Rukkwamsuk, Theera; Hendriks, Wouter H

2017-06-01

Factorial determination of the sodium (Na) requirement of heat-stressed lactating cows is hindered by accurate estimates of the Na losses through sweat. Direct studies, therefore, may be needed requiring information on the time course of healthy animals to become Na depleted and the subsequent rate of repletion. The rate of Na depletion and subsequent rate of Na repletion with two levels of dietary Na to lactating dairy cows housed under tropical conditions were investigated using the salivary Na/K. The 12 lactating cows (salivary Na/K ratio 14.6) rapidly developed clinical signs of Na deficiency, including pica, polyuria and polydipsia, reduced body weight and reduced milk yield when fed a low-Na ration (0.33 g kg -1 dry matter (DM)) for 3 weeks. Deficiency symptoms were associated with a rapid decrease in salivary Na/K ratio to cows with NaCl to a ration concentration of 1.1 or 1.6 g Na kg -1 DM for 5 weeks did not restore salivary Na/K ratio to values of >6. A daily Na intake of heat-stressed lactating cows to a ration intake of 1.6 g Na kg -1 DM was insufficient to restore Na deficiency. One week was sufficient to deplete heat-stressed lactating cows of Na, allowing for rapid dose-response studies utilizing the salivary Na/K ratio as a parameter for Na status of cows under tropical conditions. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

Drug: D06120 [KEGG MEDICUS

Lifescience Database Archive (English)

Full Text Available D06120 Drug Thyrotropin alfa (USAN/INN); Thyrotropin alfa (genetical recombination...) (JAN); Thyrotropin human alfa (genetical recombination); Thyrogen (TN) ... Therapeutic category: 7990 A

Streptomyces sp. MC10 SUŞUNUN ALFA AMİLAZ ÜRETİM KABİLİYETİNİN BELİRLENMESİ - DETERMINATION OF ABILITY OF ALPHA AMYLASE PRODUCTION BY Streptomyces sp. MC10 STRAIN

OpenAIRE

ÖZDEMİR, Ali; SIDAL, Uğur

2013-01-01

Streptomyces sp. MC10 SUŞUNUN ALFA AMİLAZ ÜRETİM KABİLİYETİNİN BELİRLENMESİAmilazlar en önemli endüstriyel enzimlerden biridir. Amilaz üreticisi olan Streptomyces MC10 suşu Manisa Celal Bayar Üniversitesinden alınan toprak örneklerinden izole edilmiştir. Bu çalışmada Streptomyces MC10 suşundan α-amilaz üretimi ve karakterizasyonu gerçekleştirilmiştir. Bu amaçla suşun besiyerinde üremesi ve α-amilaz üretme yeteneği ve aktivite gösterdiği optimum sıcaklık, pH, inkübasyon süresi ve substrat kons...

Glutathionylation-Dependence of Na+-K+-Pump Currents Can Mimic Reduced Subsarcolemmal Na+ Diffusion

Science.gov (United States)

Garcia, Alvaro; Liu, Chia-Chi; Cornelius, Flemming; Clarke, Ronald J.; Rasmussen, Helge H.

2016-01-01

The existence of a subsarcolemmal space with restricted diffusion for Na+ in cardiac myocytes has been inferred from a transient peak electrogenic Na+-K+ pump current beyond steady state on reexposure of myocytes to K+ after a period of exposure to K+-free extracellular solution. The transient peak current is attributed to enhanced electrogenic pumping of Na+ that accumulated in the diffusion-restricted space during pump inhibition in K+-free extracellular solution. However, there are no known physical barriers that account for such restricted Na+ diffusion, and we examined if changes of activity of the Na+-K+ pump itself cause the transient peak current. Reexposure to K+ reproduced a transient current beyond steady state in voltage-clamped ventricular myocytes as reported by others. Persistence of it when the Na+ concentration in patch pipette solutions perfusing the intracellular compartment was high and elimination of it with K+-free pipette solution could not be reconciled with restricted subsarcolemmal Na+ diffusion. The pattern of the transient current early after pump activation was dependent on transmembrane Na+- and K+ concentration gradients suggesting the currents were related to the conformational poise imposed on the pump. We examined if the currents might be accounted for by changes in glutathionylation of the β1 Na+-K+ pump subunit, a reversible oxidative modification that inhibits the pump. Susceptibility of the β1 subunit to glutathionylation depends on the conformational poise of the Na+-K+ pump, and glutathionylation with the pump stabilized in conformations equivalent to those expected to be imposed on voltage-clamped myocytes supported this hypothesis. So did elimination of the transient K+-induced peak Na+-K+ pump current when we included glutaredoxin 1 in patch pipette solutions to reverse glutathionylation. We conclude that transient K+-induced peak Na+-K+ pump current reflects the effect of conformation-dependent β1 pump subunit

Glutathionylation-Dependence of Na(+)-K(+)-Pump Currents Can Mimic Reduced Subsarcolemmal Na(+) Diffusion.

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Garcia, Alvaro; Liu, Chia-Chi; Cornelius, Flemming; Clarke, Ronald J; Rasmussen, Helge H

2016-03-08

The existence of a subsarcolemmal space with restricted diffusion for Na(+) in cardiac myocytes has been inferred from a transient peak electrogenic Na(+)-K(+) pump current beyond steady state on reexposure of myocytes to K(+) after a period of exposure to K(+)-free extracellular solution. The transient peak current is attributed to enhanced electrogenic pumping of Na(+) that accumulated in the diffusion-restricted space during pump inhibition in K(+)-free extracellular solution. However, there are no known physical barriers that account for such restricted Na(+) diffusion, and we examined if changes of activity of the Na(+)-K(+) pump itself cause the transient peak current. Reexposure to K(+) reproduced a transient current beyond steady state in voltage-clamped ventricular myocytes as reported by others. Persistence of it when the Na(+) concentration in patch pipette solutions perfusing the intracellular compartment was high and elimination of it with K(+)-free pipette solution could not be reconciled with restricted subsarcolemmal Na(+) diffusion. The pattern of the transient current early after pump activation was dependent on transmembrane Na(+)- and K(+) concentration gradients suggesting the currents were related to the conformational poise imposed on the pump. We examined if the currents might be accounted for by changes in glutathionylation of the β1 Na(+)-K(+) pump subunit, a reversible oxidative modification that inhibits the pump. Susceptibility of the β1 subunit to glutathionylation depends on the conformational poise of the Na(+)-K(+) pump, and glutathionylation with the pump stabilized in conformations equivalent to those expected to be imposed on voltage-clamped myocytes supported this hypothesis. So did elimination of the transient K(+)-induced peak Na(+)-K(+) pump current when we included glutaredoxin 1 in patch pipette solutions to reverse glutathionylation. We conclude that transient K(+)-induced peak Na(+)-K(+) pump current reflects the effect

A INFLUÊNCIA DO SISTEMA DE GESTÃO AMBIENTAL NA IMAGEM ORGANIZACIONAL PERANTE OS STAKEHOLDERS: UMA INDÚSTRIA TÊXTIL DE MINAS GERAIS

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Juliano Franco e Silva Amaral

2005-12-01

Full Text Available Uma característica da sociedade contemporânea é a crescente inquietação com a qualidade do ambiente natural. Grupos de pressão, cientistas, consumidores, políticos e empresas estão mais conscientes do meio ambiente e cada vez mais influentes. Na década de 1990, temas e conceitos importantes foram estudados: a teoria dos stakeholders, a ética nos negócios, a gestão ambiental, a cidadania empresarial, o desenvolvimento sustentável e a governança corporativa. Para Freeman apud Kreitlon (2003, os stakeholders podem ser definidos como qualquer grupo ou indivíduo capaz de afetar ou ser afetado pela organização. Em sentido estrito, são aqueles grupos vitais para o negócio da empresa. Segundo Moreira (2001, a organização que tem gestão ambiental é aquela que possui um departamento de meio ambiente responsável por atender às exigências dos órgãos ambientais e indicar equipamentos e dispositivos de controle ambiental apropriados à realidade do negócio e aos impactos ambientais. Por outro lado, o sistema de gestão ambiental adquire uma visão estratégica do meio ambiente. A organização deixa de agir apenas em função dos riscos e passa a perceber também as oportunidades de acordo com orientações de inserção da variável ambiental na gestão do negócio. Este artigo tem como objetivo descrever a influência da implantação do sistema de gestão ambiental na imagem de uma indústria têxtil de Minas Gerais frente aos stakeholders. A metodologia utilizada foi a revisão bibliográfica sobre sistema de gestão ambiental, a análise de documentos da Empresa Alfa e entrevistas não estruturadas com os responsáveis pela implantação da ISO 14001 e pela área de relação com investidores

A INFLUÊNCIA DO SISTEMA DE GESTÃO AMBIENTAL NA IMAGEM ORGANIZACIONAL PERANTE OS STAKEHOLDERS: UMA INDÚSTRIA TÊXTIL DE MINAS GERAIS

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Juliano Franco e Silva Amaral

2006-12-01

Full Text Available Uma característica da sociedade contemporânea é a crescente inquietação com a qualidade do ambiente natural. Grupos de pressão, cientistas, consumidores, políticos e empresas estão mais conscientes do meio ambiente e cada vez mais influentes. Na década de 1990, temas e conceitos importantes foram estudados: a teoria dos stakeholders, a ética nos negócios, a gestão ambiental, a cidadania empresarial, o desenvolvimento sustentável e a governança corporativa. Para Freeman apud Kreitlon (2003, os stakeholders podem ser definidos como qualquer grupo ou indivíduo capaz de afetar ou ser afetado pela organização. Em sentido estrito, são aqueles grupos vitais para o negócio da empresa. Segundo Moreira (2001, a organização que tem gestão ambiental é aquela que possui um departamento de meio ambiente responsável por atender às exigências dos órgãos ambientais e indicar equipamentos e dispositivos de controle ambiental apropriados à realidade do negócio e aos impactos ambientais. Por outro lado, o sistema de gestão ambiental adquire uma visão estratégica do meio ambiente. A organização deixa de agir apenas em função dos riscos e passa a perceber também as oportunidades de acordo com orientações de inserção da variável ambiental na gestão do negócio. Este artigo tem como objetivo descrever a influência da implantação do sistema de gestão ambiental na imagem de uma indústria têxtil de Minas Gerais frente aos stakeholders. A metodologia utilizada foi a revisão bibliográfica sobre sistema de gestão ambiental, a análise de documentos da Empresa Alfa e entrevistas não estruturadas com os responsáveis pela implantação da ISO 14001 e pela área de relação com investidores.

Avaliação da incidência de antracnose, do desempenho e estado nutricional de variedades de mangueira, para cultivo orgânico, na região centro-norte do Estado de São Paulo Evaluation of anthracnose incidence, development and nutritional status of mango trees varieties for organic cultivation in north center region of São Paulo state

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Juliana Altafin Galli

2009-09-01

Full Text Available A mudança do perfil do consumidor, aliada aos riscos da contaminação por agrotóxicos, tem levado à busca de alternativas ecologicamente apropriadas para produção de frutas. Os objetivos deste trabalho foram avaliar a incidência de antracnose, o desempenho e estado nutricional de variedades de mangueira conduzidas organicamente na região de Pindorama-SP. Foram utilizadas 17 variedades de mangueira. O experimento foi instalado em delineamento experimental em blocos completos ao acaso, com 17 tratamentos (variedades e seis repetições. Foi avaliada a severidade de antracnose nas folhas, através de uma escala diagramática, atribuindo-se notas aos sintomas. Foram avaliados o crescimento e o desenvolvimento (altura da planta, perímetro do tronco e da copa e o estado nutricional, mediante análise foliar, das diferentes variedades utilizadas. Através dos resultados obtidos, podem-se considerar como muito suscetíveis à antracnose as variedades Bourbon, Rocha e Rosa; e resistentes, as variedades IAC 111, Alfa, Beta e Parvin; as variedades de manga apresentaram o mesmo padrão de crescimento; as maiores alturas da planta corresponderam aos maiores diâmetros do tronco e da copa; a variedade Parvin apresentou o melhor desempenho dentre as variedades estudadas, com relação à resistência à antracnose, altura e diâmetro do caule e da copa, podendo ser recomendada ao cultivo orgânico. As variedades Omega e Alfa também apresentaram bom crescimento, podendo ser indicadas para esse cultivo, pelo menos nessa fase inicial; as variedades Surpresa e Rosa não apresentaram bom desempenho, no campo, em relação às demais, não devendo ser recomendadas para o cultivo orgânico, principalmente a variedade Rosa, bastante suscetível à antracnose. As concentrações de N, P e K foram elevadas na fase vegetativa das plantas, comparadas |à baixa concentração de Ca; houve carência de Boro em todas as variedades estudadas. A manga Rosa

Efeitos de níveis de ácido L-glutâmico e de vitamina K da dieta sobre a atividade de alfa-amilase em frangos de corte Effects of dietary levels of L-glutamic acid and vitamin K in the activity of alpha-amylase of chicks

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Flavia Escapini Fanchiotti

2005-04-01

Full Text Available Foram investigados os efeitos nutricionais de dois níveis de ácido L-glutâmico (L-Glu combinados com quatro níveis de vitamina K (Vit K sobre a atividade de alfa-amilase no quimo e pâncreas de aves de corte. Frangos de corte machos de um dia foram criados em baterias aquecidas e alimentados, à vontade, com dietas contendo todos L-aminoácidos essenciais, minerais e vitaminas (exceto Vit K até os 14 dias de idade. O experimento foi realizado em esquema fatorial, em delineamento inteiramente casualizado 2x4, com quatro repetições de oito aves cada. A dieta básica foi suplementada com 6,25 e 12,5% de L-Glu combinados com 0,02; 0,2; 20,0 e 200,0 mg de Vit K/kg de ração. Efeitos significativos de L-Glu e Vit K foram observados no quimo. A atividade específica máxima (1.005,78±245,25 UI/mg de proteína foi observada nas aves alimentadas com 6,25% de L-Glu e 20,0 mg de Vit K. Houve redução da atividade com a suplementação de 12,5% de L-Glu. No pâncreas, não houve interação entre L-Glu e Vit K, todavia, foi observado efeito de L-Glu sobre as atividades relativas, expressas em UI/g de tecido e UI/100 g de peso corporal. Os resultados sugerem que a associação entre L-Glu e Vit K interfere na atividade enzimática de alfa-amilase em aves de corte submetidas a dietas purificadas. Os resultados indicam que o nível de 12,5% de L-Glu associado aos diferentes níveis de vitamina K reduziu a atividade enzimática.The effects of two levels of L-glutamic acid (L-Glu combined with four levels of vitamin K (Vit K were studied with the objective of evaluating the activities of alpha-amylase in the chyme and pancreas of chicks. Day-old male broilers were reared in eletrically heated batteries, fed with purified amino acids diets, minerals and vitamins (except Vit K at the requirement levels, for 14 days. Feed and water were provided ad libitum. The experimental design was a factorial 2x4 with four replicates with eight chicks each The

Crystal structure of a Na+-bound Na+,K+-ATPase preceding the E1P state.

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Kanai, Ryuta; Ogawa, Haruo; Vilsen, Bente; Cornelius, Flemming; Toyoshima, Chikashi

2013-10-10

Na(+),K(+)-ATPase pumps three Na(+) ions out of cells in exchange for two K(+) taken up from the extracellular medium per ATP molecule hydrolysed, thereby establishing Na(+) and K(+) gradients across the membrane in all animal cells. These ion gradients are used in many fundamental processes, notably excitation of nerve cells. Here we describe 2.8 Å-resolution crystal structures of this ATPase from pig kidney with bound Na(+), ADP and aluminium fluoride, a stable phosphate analogue, with and without oligomycin that promotes Na(+) occlusion. These crystal structures represent a transition state preceding the phosphorylated intermediate (E1P) in which three Na(+) ions are occluded. Details of the Na(+)-binding sites show how this ATPase functions as a Na(+)-specific pump, rejecting K(+) and Ca(2+), even though its affinity for Na(+) is low (millimolar dissociation constant). A mechanism for sequential, cooperative Na(+) binding can now be formulated in atomic detail.

Na+-H+ exchange and Na+-dependent transport systems in streptozotocin diabetic rat kidneys

International Nuclear Information System (INIS)

El-Seifi, S.; Freiberg, J.M.; Kinsella, F.J.; Cheng, L.; Sacktor, B.

1987-01-01

The streptozotocin-induced diabetic rat was used to test the hypothesis that Na + -H + exchange activity in the proximal tubule luminal membrane would be increased in association with renal hypertrophy, altered glomerular hemodynamics, enhanced filtered load and tubular reabsorption of 22 Na + , and stimulated 22 Na= pump activity in the basolateral membrane, previously reported characteristics of this experimental animal model. Amiloride-sensitive H + gradient-dependent Na + uptake and Na + gradient-dependent H + flux were increased in brush-border membrane vesicles from the streptozotocin-treated animals. Na + gradient-dependent uptakes of phosphate, D-glucose, L-proline, and myoinositol were decreased in the drug-induced diabetic animals. These membrane transport alterations were not found when the streptozotocin-diabetic animals were treated with insulin

CLINICAL AND IMMUNOLOGICAL EFFICACY OF THE RECOMBINANT INTERFERON alfa-2b OF ACUTE EPSTEIN-BARR VIRAL MONONUCLEOSIS IN PRESCHOOL CHILDREN

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V. N. Timchenko

2016-01-01

Full Text Available Conducted clinical and immunological examination of 51 children with acute Epstein-Barr viral mononucleosis (EBV in age from 1 year to 7 years. All children diagnosed with a moderate degree of the disease. In the treatment of 25 people (comparison group used the standard treatment (pathogenetic, symptomatic, treatment 26 people (main group included the use as antiviral and immunotropic means of the preparation of human recombinant interferon Alfa-2b in the form of rectal suppositories — VIFERON®. The immunological survey was conducted in dynamics: at the height of the disease and in the convalescence period. The blood was determined indicators of cellular immunity (leukocytes, lymphocytes, CD3+, CD4+, CD8+, CD56+, HLAII+, CD95+, CD1 6+, CD25+, the concentration of IFN-a, IFN-y, IL-4 levels in spontaneous and induced production, in the serum. At milestones children of the main group noted rapid regression of clinical symptoms, normalization of body temperature, reducing intoxication, positive dynamics lymphoproliferative syndrome, a significant reduction in bed-days, no layering respiratory viral infections. Interferon had also expressed a positive impact on the changed parameters of cellular immunity and cytokine links. At the same time, 80% of patients after basic treatment is established the predominance of Th2 type immune response, indicating a high risk of developing chronic course of EBV-mononucleosis.

The two C-terminal tyrosines stabilize occluded Na/K pump conformations containing Na or K ions.

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Vedovato, Natascia; Gadsby, David C

2010-07-01

Interactions of the three transported Na ions with the Na/K pump remain incompletely understood. Na/K pump crystal structures show that the extended C terminus of the Na,K-adenosine triphosphatase (ATPase) alpha subunit directly contacts transmembrane helices. Deletion of the last five residues (KETYY in almost all Na/K pumps) markedly lowered the apparent affinity for Na activation of pump phosphorylation from ATP, a reflection of cytoplasmic Na affinity for forming the occluded E1P(Na3) conformation. ATPase assays further suggested that C-terminal truncations also interfere with low affinity Na interactions, which are attributable to extracellular effects. Because extracellular Na ions traverse part of the membrane's electric field to reach their binding sites in the Na/K pump, their movements generate currents that can be monitored with high resolution. We report here electrical measurements to examine how Na/K pump interactions with extracellular Na ions are influenced by C-terminal truncations. We deleted the last two (YY) or five (KESYY) residues in Xenopus laevis alpha1 Na/K pumps made ouabain resistant by either of two kinds of point mutations and measured their currents as 10-mM ouabain-sensitive currents in Xenopus oocytes after silencing endogenous Xenopus Na/K pumps with 1 microM ouabain. We found the low affinity inhibitory influence of extracellular Na on outward Na/K pump current at negative voltages to be impaired in all of the C-terminally truncated pumps. Correspondingly, voltage jump-induced transient charge movements that reflect pump interactions with extracellular Na ions were strongly shifted to more negative potentials; this signals a several-fold reduction of the apparent affinity for extracellular Na in the truncated pumps. Parallel lowering of Na affinity on both sides of the membrane argues that the C-terminal contacts provide important stabilization of the occluded E1P(Na3) conformation, regardless of the route of Na ion entry into the

CHANGES IN TUMOR NECROSIS FACTOR ALFA DURING TREATMENT OF PATIENTS WITH MULTIPLE SCLEROSIS BY TRANSIMMUNIZATION METHOD

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A. V. Kil'dyushevskiy

2016-01-01

Full Text Available Background: Despite the availability of a  large number of treatments for multiple sclerosis with various targets, these treatments are not always effective. According to the literature, experimental studies have shown a  significant decrease in tumor necrosis factor alfa (TNF-α with the use of extracorporeal photochemotherapy. Aim: To assess changes in TNF-α in patients with multiple sclerosis during treatment with transimmunization. Materials and methods: The study recruited 13 adult patients with multiple sclerosis. Serum TNF-α was measured by immunochemiluminescence analysis (IMMULITE 1000, Siemens. The patients were treated by transimmunization, i.e. a  modified photopheresis. Two hours before the procedure, Ammifurin (8-methoxypsoralene was administered to all the patients, then their mononuclear cells were isolated under PBSC protocol with Haemonetics MCS+ cell separator. Thereafter, mononuclear cells were irradiated with ultraviolet for 90  minutes and incubated for 20 hours at 37 °С. The next day the cells were re-infused to the patients. The procedure was performed 2  times per week for 6  months, then once per 4  months. Results: Before transimmunization, mean TNF-α level in adult patients with multiple sclerosis was 9.958±0.812  pg/mL (normal, below 8.1 pg/mL. After transimmunization, its level was 6.992±0.367  pg/mL (р<0.05. Conclusion: Ultraviolet irradiation of peripheral blood monocytes with their subsequent incubation (transimmunization led to a 30% decrease of serum TNF-α in patients with multiple sclerosis. This indicates a suppressive effect of transimmunization on TNF-α. Hence, in patients with multiple sclerosis transimmunization exerts an anti-inflammatory effect.

Andexanet alfa effectively reverses edoxaban anticoagulation effects and associated bleeding in a rabbit acute hemorrhage model

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Lu, Genmin; Pine, Polly; Leeds, Janet M.; DeGuzman, Francis; Pratikhya, Pratikhya; Lin, Joyce; Malinowski, John; Hollenbach, Stanley J.; Curnutte, John T.

2018-01-01

Introduction Increasing use of factor Xa (FXa) inhibitors necessitates effective reversal agents to manage bleeding. Andexanet alfa, a novel modified recombinant human FXa, rapidly reverses the anticoagulation effects of direct and indirect FXa inhibitors. Objective To evaluate the ability of andexanet to reverse anticoagulation in vitro and reduce bleeding in rabbits administered edoxaban. Materials and methods In vitro studies characterized the interaction of andexanet with edoxaban and its ability to reverse edoxaban-mediated anti-FXa activity. In a rabbit model of surgically induced, acute hemorrhage, animals received edoxaban vehicle+andexanet vehicle (control), edoxaban (1 mg/kg)+andexanet vehicle, edoxaban+andexanet (75 mg, 5-minute infusion, 20 minutes after edoxaban), or edoxaban vehicle+andexanet prior to injury. Results Andexanet bound edoxaban with high affinity similar to FXa. Andexanet rapidly and dose-dependently reversed the effects of edoxaban on FXa activity and coagulation pharmacodynamic parameters in vitro. In edoxaban-anticoagulated rabbits, andexanet reduced anti-FXa activity by 82% (from 548±87 to 100±41 ng/ml; P<0.0001), mean unbound edoxaban plasma concentration by ~80% (from 100±10 to 21±6 ng/ml; P<0.0001), and blood loss by 80% vs. vehicle (adjusted for control, 2.6 vs. 12.9 g; P = 0.003). The reduction in blood loss correlated with the decrease in anti-FXa activity (r = 0.6993, P<0.0001) and unbound edoxaban (r = 0.5951, P = 0.0035). Conclusion These data demonstrate that andexanet rapidly reversed the anticoagulant effects of edoxaban, suggesting it could be clinically valuable for the management of acute and surgery-related bleeding. Correlation of blood loss with anti-FXa activity supports the use of anti-FXa activity as a biomarker for assessing anticoagulation reversal in clinical trials. PMID:29590221

Purificação, caracterização físico-química e atividade anticoagulante de glicosaminoglicanos isolados da pele de tilápia do Nilo (Oreochromis niloticus - doi: 10.4025/actascitechnol.v33i3.9400

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José Ariévilo Gurgel Rodrigues

2011-07-01

Full Text Available Os glicosaminoglicanos (GAGs constituem a matriz extracelular de tecidos animais e os resíduos do processamento de peixes comercialmente cultivados podem oferecer novos agentes anticoagulantes em substituição à heparina (HEP nas ciências médicas. Objetivou-se purificar, caracterizar físico-quimicamente e avaliar a atividade anticoagulante dos GAGs isolados da pele da tilápia do Nilo, Oreochromis niloticus. Os GAGs foram extraídos com papaína em tampão acetato de sódio 100 mM (pH 5,0 contendo cisteína e EDTA (5 mM, seguido por cromatografia de troca iônica em coluna de DEAE-celulose, utilizando um gradiente de NaCl. As frações obtidas foram liofilizadas e submetidas à eletroforese em gel de agarose a 0,5%. A atividade anticoagulante foi avaliada por meio do tempo de tromboplastina parcial ativada (TTPA, usando-se plasma humano normal e HEP padrão (193 UI mg-1. O perfil cromatográfico mostrou a separação de duas diferentes frações de GAGs (F I e F II, eluídas com 0,50 e 0,75 M de NaCl, respectivamente, revelando, por eletroforese, frações distintas em densidade de cargas, porém ambas mostrando GAGs com mobilidades semelhantes ao dermatam sulfato (DS padrão, sugerindo-se a identificação preliminar desse GAG. Os GAGs modificaram o TTPA, cujas atividades perfizeram, respectivamente, 4,72 (F I e 23,80 (F II UI mg-1, e expressaram-se dose-dependente. Portanto, o DS anticoagulante da pele de O. niloticus sugere uma fonte interessante para estudos posteriores de atividade antitrombótica.

Anion-coupled Na efflux mediated by the human red blood cell Na/K pump

International Nuclear Information System (INIS)

Dissing, S.; Hoffman, J.F.

1990-01-01

The red cell Na/K pump is known to continue to extrude Na when both Na and K are removed from the external medium. Because this ouabain-sensitive flux occurs in the absence of an exchangeable cation, it is referred to as uncoupled Na efflux. This flux is also known to be inhibited by 5 mM Nao but to a lesser extent than that inhibitable by ouabain. Uncoupled Na efflux via the Na/K pump therefore can be divided into a Nao-sensitive and Nao-insensitive component. We used DIDS-treated, SO4-equilibrated human red blood cells suspended in HEPES-buffered (pHo 7.4) MgSO4 or (Tris)2SO4, in which we measured 22Na efflux, 35SO4 efflux, and changes in the membrane potential with the fluorescent dye, diS-C3 (5). A principal finding is that uncoupled Na efflux occurs electroneurally, in contrast to the pump's normal electrogenic operation when exchanging Nai for Ko. This electroneutral uncoupled efflux of Na was found to be balanced by an efflux of cellular anions. (We were unable to detect any ouabain-sensitive uptake of protons, measured in an unbuffered medium at pH 7.4 with a Radiometer pH-STAT.) The Nao-sensitive efflux of Nai was found to be 1.95 +/- 0.10 times the Nao-sensitive efflux of (SO4)i, indicating that the stoichiometry of this cotransport is two Na+ per SO4=, accounting for 60-80% of the electroneutral Na efflux. The remainder portion, that is, the ouabain-sensitive Nao-insensitive component, has been identified as PO4-coupled Na transport and is the subject of a separate paper. That uncoupled Na efflux occurs as a cotransport with anions is supported by the result, obtained with resealed ghosts, that when internal and external SO4 was substituted by the impermeant anion, tartrate i,o, the efflux of Na was inhibited 60-80%. This inhibition could be relieved by the inclusion, before DIDS treatment, of 5 mM Cli,o

Improved inflammatory activity with peginterferon alfa-2b maintenance therapy in non-cirrhotic prior non-responders: a randomized study.

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Poynard, Thierry; Bruix, Jordi; Schiff, Eugene R; Diago, Moises; Berg, Thomas; Moreno-Otero, Ricardo; Lyra, Andre C; Carrilho, Flair; Griffel, Louis H; Boparai, Navdeep; Jiang, Ruiyun; Burroughs, Margaret; Brass, Clifford A; Albrecht, Janice K

2013-03-01

Therapeutic options for patients failing hepatitis C retreatment are limited. EPIC(3) included a prospective trial assessing long-term peginterferon alfa-2b (PegIFNα-2b) maintenance therapy in patients with METAVIR fibrosis scores (MFS) of F2 or F3 who previously failed hepatitis C retreatment. Patients with F2/F3 MFS who failed retreatment were randomized to PegIFNα-2b (0.5 μg/kg/week, n=270) or observation (n=270) for 36 months. Blinded liver biopsies obtained before retreatment and after maintenance therapy were evaluated using MFS and activity scores, and confirmatory testing was performed using FibroTest and ActiTest. In total, 348 patients had paired biopsies: 192 patients had missing post-treatment biopsies and were considered as having no change in fibrosis/activity scores. In total, 16% of patients receiving PegIFNα-2b and 11% of observation patients had improvement in MFS (p=0.32). More PegIFNα-2b than observation patients had improvement in activity score (20% vs. 9%; p 2.5 years, improvement in MFS or activity score was more common with PegIFNα-2b than observation (21% vs. 14%, p=0.08 and 26% vs. 10%, p 2.5 years. Both FibroTest and ActiTest were significantly improved during maintenance therapy. Copyright © 2012 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.

Diversidad alfa y beta de los artrópodos en diferentes ambientes del Parque Nacional Los Cardones, Salta (Argentina

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María Belén Cava

2013-12-01

Full Text Available El Parque Nacional Los Cardones ubicado en la provincia de Salta protege las ecorregiones de Puna, Monte y Pastizal Altoandino Nublado de la Yungas. Nuestro objetivo es evaluar la diversidad alfa y beta de artrópodos en las ecorregiones del Parque. Se muestrearon tres sitios en cada ecorregión utilizando Pit-fall y G-Vac. Se calcularon estimadores no paramétricos de riqueza de especies, la diversidad entre ecorregiones se comparó a través de los “perfiles de diversidad” y “números efectivos de especies”, se analizó el recambio de especies y la complementariedad entre las ecorregiones y sitios de una misma ecorregión. Se registraron 469 spp/morfoespecies, la diversidad en el Parque fue alta, siendo mayor en los Pastizal Altoandino Nublado, y la más baja en la Puna. El inventario llegó al 81% de completitud. El recambio de especies entre ecorregiones fue alto, de esa manera los diferentes ambientes del Parque contribuyen a conservar la diversidad regional de artrópodos. Las ensambles de artrópodos en sitios de una misma ecorregión mostraron mayor similitud entre sí que con sitios más distantes. Se pudo reconocer tres grupos de artrópodos (arañas, dípteros e himenópteros que son diversos y abundantes, los que podrían ser utilizados en futuros estudios como grupos focales.

Valor do emprego do questionário WHOQOL-BREF na avaliação da qualidade de vida de pais de crianças com asma

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Cristian Roncada

2015-09-01

Full Text Available ResumoObjetivo:Avaliar a qualidade de vida (QV de pais de crianças asmáticas e analisar a consistência interna do instrumento genérico de qualidade de vida World Health Organization Quality of Life, versão abreviada (WHOQOL-BREF.Métodos:Foi avaliada a QV de pais de crianças asmáticas e hígidas entre oito-16 anos, por meio do questionário genérico WHOQOL-BREF. Foi avaliada também a consistência interna, por meio do alfa de Cronbach (αC, para determinar se o instrumento tem boa validade para o público alvo.Resultados:Participaram do estudo 162 indivíduos, com idade média de 43,8±13,6 anos, dos quais 104 eram do sexo feminino (64,2% e 128 casados (79,0%. Na avaliação do nível de qualidade de vida, o grupo de pais de crianças saudáveis apresentou escores acima do grupo de pais de asmáticos nos quatro domínios do instrumento (físico, psicológico, social e meio ambiente que indicam melhor qualidade de vida. Na análise de consistência interna, o WHOQOL-BREF obteve valores de αC=0,86 pontos para o grupo de pais de asmáticos e 0,88 para o grupo de pais de hígidos.Conclusões:Pais de crianças asmáticas apresentam comprometimento da qualidade de vida em função da doença de seus filhos. Além disso, o WHOQOL-BREF, mesmo sendo um instrumento genérico, se mostrou prático e eficiente para avaliar a qualidade de vida de pais de crianças asmáticas.

Na5NbO5 and Na5TaO5 phases

International Nuclear Information System (INIS)

Darriet, J.; Maazaz, A.; Bouloux, J.C.; Delmas, C.

1982-01-01

New ternary oxides of formulas Na 5 NbO 5 and Na 5 TaO 5 have been prepared. They crystallize in the monoclinic system (space group C2/c). The crystal structure of Na 5 NbO 5 has been determined. It derives from a NaCl-type structure by ordering of the cations and of the oxygen vacancies in the anionic sublattice, the corresponding formula being Nasub(5/6)Nbsub(1/6)Osub(5/6)vacant sub(1/6). Sodium and niobium have a distorted square-pyramidal surrounding. (author)

Synthesis of Na-A and/or Na-X zeolite/porous carbon composites from carbonized rice husk

International Nuclear Information System (INIS)

Katsuki, Hiroaki; Komarneni, Sridhar

2009-01-01

Na-A and/or Na-X zeolite/porous carbon composites were prepared under hydrothermal conditions by NaOH dissolution of silica first from carbonized rice husk followed by addition of NaAlO 2 and in situ crystallization of zeolites i.e., using a two-step process. When a one-step process was used, both Na-A and Na-X zeolites crystallized on the surface of carbon. Na-A or Na-X zeolite crystals were prepared on the porous carbonized rice husk at 90 deg. C for 2-6 h by changing the SiO 2 /Al 2 O 3 , H 2 O/Na 2 O and Na 2 O/SiO 2 molar ratios of precursors in the two-step process. The surface area and NH 4 + -cation exchange capacity (CEC) of Na-A zeolite/porous carbon were found to be 171 m 2 /g and 506 meq/100 g, respectively, while those of Na-X zeolite/porous carbon composites were 676 m 2 /g and 317 meq/100 g, respectively. Na-A and Na-X zeolites are well-known microporous and hydrophilic materials while carbonized rice husk was found to be mesoporous (pores of ∼3.9 nm) and hydrophobic. These hybrid microporous-mesoporous and hydrophilic-hydrophobic composites are expected to be useful for decontamination of metal cations as well as organic contaminants simultaneously. - Graphical Abstract: Novel Na-X zeolite/porous carbon composite.

Equilibrium chemical transformations in NaPO3 + NaCl melts

International Nuclear Information System (INIS)

Kovarskaya, E.N.; Rodionov, Yu.I.

1988-01-01

Because of the problems of the burial of solidified radioactive wastes into different geological rock formations, in particular into massives of rock-salt, the state of molten polyphosphate-chloride mixtures (taking into account the chemical character of the interaction of their components) for a prolonged period of time. The equilibrium products of the reaction in the NaPO 3 -NaCl system were studied in melts in air in the composition range of 30-70 mole % NaCl. It was shown that with increase in the NaCl content in the mixtures, the polyphosphate gradually depolymerizes to sodium tri-, di-, and monophosphates, and the composition of the equilibrium melts is dependent only on the ratio between the components in the initial molten mixtures. The time until the equilibrium is attained is shorter, the higher is the experimental temperature

Long-Range Effects of Na(+) Binding in Na,K-ATPase Reported by ATP.

Science.gov (United States)

Middleton, David A; Fedosova, Natalya U; Esmann, Mikael

2015-12-01

This paper addresses the question of long-range interactions between the intramembranous cation binding sites and the cytoplasmic nucleotide binding site of the ubiquitous ion-transporting Na,K-ATPase using (13)C cross-polarization magic-angle spinning (CP-MAS) solid-state nuclear magnetic resonance. High-affinity ATP binding is induced by the presence of Na(+) as well as of Na-like substances such as Tris(+), and these ions are equally efficient promoters of nucleotide binding. CP-MAS analysis of bound ATP with Na,K-ATPase purified from pig kidney membranes reveals subtle differences in the nucleotide interactions within the nucleotide site depending on whether Na(+) or Tris(+) is used to induce binding. Differences in chemical shifts for ATP atoms C1' and C5' observed in the presence of Na(+) or Tris(+) suggest alterations in the residues surrounding the bound nucleotide, hydrogen bonding, and/or conformation of the ribose ring. This is taken as evidence of a long-distance communication between the Na(+)-filled ion sites in the membrane interior and the nucleotide binding site in the cytoplasmic domain and reflects the first conformational change ultimately leading to phosphorylation of the enzyme. Stopped-flow fluorescence measurements with the nucleotide analogue eosin show that the dissociation rate constant for eosin is larger in Tris(+) than in Na(+), giving kinetic evidence of the difference in structural effects of Na(+) and Tris(+). According to the recent crystal structure of the E1·AlF4(-)·ADP·3Na(+) form, the coupling between the ion binding sites and the nucleotide side is mediated by, among others, the M5 helix.

Interaction between Na+/K+-pump and Na+/Ca2+-exchanger modulates intercellular communication.

Science.gov (United States)

Matchkov, Vladimir V; Gustafsson, Helena; Rahman, Awahan; Briggs Boedtkjer, Donna M; Gorintin, Sarah; Hansen, Anne Kirstine; Bouzinova, Elena V; Praetorius, Helle A; Aalkjaer, Christian; Nilsson, Holger

2007-04-13

Ouabain, a specific inhibitor of the Na(+)/K(+)-pump, has previously been shown to interfere with intercellular communication. Here we test the hypothesis that the communication between vascular smooth muscle cells is regulated through an interaction between the Na(+)/K(+)-pump and the Na(+)/Ca(2+)-exchanger leading to an increase in the intracellular calcium concentration ([Ca(2+)](i)) in discrete areas near the plasma membrane. [Ca(2+)](i) in smooth muscle cells was imaged in cultured rat aortic smooth muscle cell pairs (A7r5) and in rat mesenteric small artery segments simultaneously with force. In A7r5 coupling between cells was estimated by measuring membrane capacitance. Smooth muscle cells were uncoupled when the Na(+)/K(+)-pump was inhibited either by a low concentration of ouabain, which also caused a localized increase of [Ca(2+)](i) near the membrane, or by ATP depletion. Reduction of Na(+)/K(+)-pump activity by removal of extracellular potassium ([K(+)](o)) also uncoupled cells, but only after inhibition of K(ATP) channels. Inhibition of the Na(+)/Ca(2+)-exchange activity by SEA0400 or by a reduction of the equilibrium potential (making it more negative) also uncoupled the cells. Depletion of intracellular Na(+) and clamping of [Ca(2+)](i) at low concentrations prevented the uncoupling. The experiments suggest that the Na(+)/K(+)-pump may affect gap junction conductivity via localized changes in [Ca(2+)](i) through modulation of Na(+)/Ca(2+)-exchanger activity.

Peginterferon Lambda-1a Is Associated with a Low Incidence of Autoimmune Thyroid Disease in Chronic Hepatitis C.

Science.gov (United States)

Fredlund, Paul; Hillson, Jan; Gray, Todd; Shemanski, Lynn; Dimitrova, Dessislava; Srinivasan, Subasree

2015-11-01

Peginterferon alfa (alfa) increases the risk of autoimmune disease. Peginterferon lambda-1a (Lambda) acts through a receptor with a more liver-specific distribution compared to the alfa receptor. In a phase-2b study, 525 treatment-naive patients with chronic hepatitis C virus (HCV) infection received ribavirin and Lambda interferon (120, 180, or 240 μg) or alfa interferon (180 μg) for 24 (genotypes 2 and 3) or 48 (genotypes 1 and 4) weeks. Retrospective analysis found that adverse events of MedDRA-coded thyroid dysfunction and abnormal levels of thyroid-stimulating hormone (TSH) were significantly more frequent with alfa versus Lambda (12% versus 2.6% and 15.2% versus 3.4%, respectively, both PLambda recipients with abnormal TSH had levels below the lower limit of normal; the frequency of low and high TSH was similar in alfa recipients with abnormal TSH. Blinded review by an endocrinologist found that new-onset primary hypothyroidism or painless thyroiditis was less frequent with Lambda versus alfa (0.5% and 1.8% versus 5.3% and 7.5%, respectively, PLambda/ribavirin experienced fewer adverse events of thyroid dysfunction compared with patients treated with alfa/ribavirin.

Intracellular Na(+) and metabolic modulation of Na/K pump and excitability in the rat suprachiasmatic nucleus neurons.

Science.gov (United States)

Wang, Yi-Chi; Yang, Jyh-Jeen; Huang, Rong-Chi

2012-10-01

Na/K pump activity and metabolic rate are both higher during the day in the suprachiasmatic nucleus (SCN) that houses the circadian clock. Here we investigated the role of intracellular Na(+) and energy metabolism in regulating Na/K pump activity and neuronal excitability. Removal of extracellular K(+) to block the Na/K pump excited SCN neurons to fire at higher rates and return to normal K(+) to reactivate the pump produced rebound hyperpolarization to inhibit firing. In the presence of tetrodotoxin to block the action potentials, both zero K(+)-induced depolarization and rebound hyperpolarization were blocked by the cardiac glycoside strophanthidin. Ratiometric Na(+) imaging with a Na(+)-sensitive fluorescent dye indicated saturating accumulation of intracellular Na(+) in response to pump blockade with zero K(+). The Na(+) ionophore monensin also induced Na(+) loading and hyperpolarized the membrane potential, with the hyperpolarizing effect of monensin abolished in zero Na(+) or by pump blockade. Conversely, Na(+) depletion with Na(+)-free pipette solution depolarized membrane potential but retained residual Na/K pump activity. Cyanide inhibition of oxidative phosphorylation blocked the Na/K pump to depolarize resting potential and increase spontaneous firing in most cells, and to raise intracellular Na(+) levels in all cells. Nonetheless, the Na/K pump was incompletely blocked by cyanide but completely blocked by iodoacetate to inhibit glycolysis, indicating the involvement of both oxidative phosphorylation and glycolysis in fueling the Na/K pump. Together, the results indicate the importance of intracellular Na(+) and energy metabolism in regulating Na/K pump activity as well as neuronal excitability in the SCN neurons.

WISC-III e WAIS-III na avaliação da inteligência de cegos

OpenAIRE

Nascimento, Elizabeth do; Flores-Mendoza, Carmen Elvira

2007-01-01

Diante da escassez de pesquisas nacionais e de testes psicológicos destinados a avaliar pessoas cegas, desenvolveu-se um estudo psicométrico com as escalas verbais dos testes WISC-III e WAIS-III. Após as adaptações de alguns estímulos e das instruções, os testes foram aplicados em crianças (N = 120) e adultos (N = 52) residentes em Belo Horizonte. Os resultados indicaram que as escalas verbais modificadas apresentam uma boa consistência interna (alfa> 0,80). Além disso, a investigação da vali...

Synthesis of zeolites Na-A and Na-X from tablet compressed and calcinated coal fly ash

Science.gov (United States)

Hu, Tao; Gao, Wenyan; Liu, Xin; Zhang, Yifu; Meng, Changgong

2017-10-01

Zeolites Na-A and Na-X are important synthetic zeolites widely used for separation and adsorption in industry. It is of great significance to develop energy-efficient routines that can synthesize zeolites Na-A and Na-X from low-cost raw materials. Coal fly ash (CFA) is the major residue from the combustion of coal and biomass containing more than 85% SiO2 and Al2O3, which can readily replace the conventionally used sodium silicate and aluminate for zeolite synthesis. We used Na2CO3 to replace the expensive NaOH used for the calcination of CFA and showed that tablet compression can enhance the contact with Na2CO3 for the activation of CFA through calcination for the synthesis of zeolites Na-A and Na-X under mild conditions. We optimized the control variables for zeolite synthesis and showed that phase-pure zeolite Na-A can be synthesized with CFA at reactant molar ratio, hydrothermal reaction temperature and reaction time of 1.3Na2O: 0.6Al2O3: 1SiO2: 38H2O at 80°C for 6 h, respectively, while phase-pure zeolite Na-X can be synthesized at 2.2Na2O: 0.2Al2O3: 1SiO2: 88H2O at 100°C for 8 h, respectively. The composition, morphology, specific surface area, vibration spectrum and thermogravimetry of synthesized Na-A and Na-X were further characterized.

Síndrome da resposta inflamatória sistêmica na circulação extracorpórea: papel das interleucinas

Directory of Open Access Journals (Sweden)

MOURA Helmiton Vieira de

2001-01-01

Full Text Available A síndrome da resposta inflamatória sistêmica (SIRS na cirurgia cardíaca ocorre, com maior freqüência, com o uso da CEC. Isto se deve ao contato do sangue com superfícies não endoteliais e, mais tarde, à reperfusão. Esse contato leva à liberação de componentes moleculares, dos quais as anafilotoxinas ativadas pelo fator de complemento, que estimulam a liberação de citocinas pró-inflamatórias, tais como a IL-1, IL-6, FNT-a. Estas interleucinas são responsáveis por indução de febre, neutrofilia, com aumento da adesividade entre neutrófilos e miócitos e/ou endotélio, e agem de forma sinergética estimulando a produção de outras interleucinas pelos monócitos e leucócitos (IL-6, IL-8. Observa-se que a isquemia intestinal, decorrente do uso da CEC, ocasiona a produção de IL-6, FNT-a, IL-1 no epitélio intestinal, e a reperfusão pós-CEC provoca a expressão de IL-1 e FNT-a no endotélio microvascular e pode gerar uma disfunção que danifica os cardiomiócitos. Seguindo a cascata de reações pós- CEC, a síndrome do distress respiratório do adulto é mediada pela IL-8, encontrada a nível alveolar. Cérebro e fígado também podem ser afetados pelos fenômenos causados pela isquemia/reperfusão e se demonstra que as interlucinas, uma vez mais (IL-8 e IL-13, respectivamente, têm um papel importante na série de eventos que ocorre. As técnicas de CEC bem como a anestesia podem influenciar a atividade das interleucinas. Não há um consenso sobre terapias preventivas ou de tratamento das complicações advindas da SIRS. Intervenção a nível dos mediadores da inflamação, procedimento de descontaminação digestiva, uso de drogas como a indometacina ou esteróides são opções possíveis. A regulação do processo inflamatório, de um modo geral, depende do equilíbrio entre citocinas pró- e anti-inflamatórias. Estas últimas são detectáveis no indivíduo normal, enquanto que as pró-inflamatórias apenas se

The CERES / NA45 experiment

CERN Multimedia

Laurent Guiraud

2000-01-01

Ceres is one of the second generation heavy ion experiments at CERN's SPS. It is dedicated to the study of electron-positron pairs in relativistic nuclear collisions. NA45 is one of the seven experiments (NA44, NA45, NA49, NA50, NA52, WA97/NA57 and WA98) involved in CERN's Heavy Ion programme which provided evidence for the existence of a new state of matter, the quark-gluon plasma. In this state, quarks, instead of being bound up into more complex particles such as protons and neutrons, are liberated and roam freely. Theory predicts that this state must have existed at about 10 microseconds after the Big Bang, before the formation of matter as we know it today.

Focusing on variation: methods and applications of the concept of beta diversity in aquatic ecosystems Focando na variação: métodos e aplicações do conceito de diversidade beta em ecossistemas aquáticos

Directory of Open Access Journals (Sweden)

Adriano Sanches Melo

2011-09-01

sistemas naturais e fazer predições. Em ecologia de comunidades, a maioria dos estudos foca na diversidade local de espécies (diversidade alfa, e apenas recentemente ecólogos passaram a dar atenção apropriada a variação na composição de comunidades entre sítios amostrais (diversidade beta. Isto acontece apesar do fato das primeiras tentativas de estimar diversidade beta terem sido feitas pelos trabalhos pioneiros de Koch e Whittaker na década de 1950. Houve significativo progresso na última década no desenvolvimento tanto de métodos como de hipóteses sobre a origem e manutenção da variação na composição de comunidades. Por exemplo, métodos estão disponíveis para particionar a diversidade total de uma região (diversidade gama num componente local (alfa e diversos componentes de diversidade beta, cada um referente a uma escala na hierarquia. A popularização da chamada abordagem de dados brutos (raw-data approach (baseada em técnicas de ordenação parcial restrita e abordagem baseada em distância (distance-based approach (baseada em correlações de matrizes de dissimilaridade/distância tem possibilitado a avaliação de hipóteses sobre os padrões de diversidade beta. De maneira geral, estas hipóteses são baseadas nas teorias de nicho e neutra, e levam em consideração o papel do ambiente e do espaço (ou combinação deles na determinação das metacomunidades. Estudos recentes avaliaram estes temas em grande variedade de escalas espaciais e temporais, de habitats e grupos taxonômicos. Adicionalmente, histórias de vida e traços funcionais das espécies tais como habilidades de dispersão e raridade estão começando a ser consideradas nos estudos de diversidade beta. Neste artigo nós revisamos brevemente algumas destas novas ferramentas e abordagens e as ilustramos com estudos de caso em ecossistemas aquáticos.

Evaluation of total carotenoids, alpha- and beta-carotene in carrots (Daucus carota L. during home processing Avaliação de carotenóides totais, alfa e beta-caroteno em cenoura (Daucus carota L. durante processamento a nível doméstico

Directory of Open Access Journals (Sweden)

Helena Maria PINHEIRO-SANT’ANA

1998-04-01

Full Text Available This study aims to analyze the influence of dehydration and different preparation methods during home processing related toalpha-carotene, beta-carotene and total carotenoids stability in carrots. Vitamin A values were evaluated after different treatments. Thus, carrots were submitted to steam cooking, water cooking with and without pressure, moist/dry cooking and conventional dehydration. Determination of alpha- and beta-carotenes was made by High-Performance Liquid Chromatography (HPLC (conditions were developed by us using spectrophotometric detection visible-UV at 470 nm; a RP-18 column and methanol: acetonitrile: ethyl acetate (80: 10: 10 as mobile phase. Total carotenoids quantification was made by 449 nm spectrophotometer. The retention of the analyzed carotenoids ranged from 60.13 to 85.64%. Water cooking without pressure promoted higher retention levels of alpha- and beta-carotene and vitamin A values, while water cooking with pressure promoted higher retention levels of total carotenoids. Dehydration promoted the highest carotenoid losses. The results showed that, among the routinely utilized methods under domestic condition, cooking without pressure, if performed under controlled time and temperature, is the best method as it reduces losses in the amount of alpha- and beta-carotene, the main carotenoids present in the carrots. Despite the significant carotenoid losses, carrots prepared through domestic methods, remain a rich source of provitamin A.O presente estudo teve como objetivo analisar a influência da desidratação e de diferentes métodos de preparo a nível doméstico sobre a estabilidade de alfa-caroteno, beta-caroteno e carotenóides totais em cenouras. Os valores de vitamina A foram avaliados após os diferentes tratamentos. Para tanto, amostras de cenoura foram submetidas à cocção a vapor, cocção em água com e sem pressão, cocção úmida/seca e à desidratação convencional. Para a determinação de alfa e beta

Actividad biológica de la prostaglandina F2 alfa y del metil carboprost en el fundus gástrico aislado de rata

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Roberto Rojas Martínez

1997-08-01

Full Text Available Se comparó la potencia contráctil de una muestra del éster metílico de 15 metil prostaglandina F2 alfa (metil carboprost (I, producida por la Industria Farmacéutica Cubana, con una muestra comercial de prostaglandina F2 alfa (II, de la firma Chinoin, mediante el ensayo de los "tres puntos". También se comparó la duración de la contracción inducida por dichas muestras utilizando un análisis de regresión lineal de la relajación observada en función del tiempo, luego de suprimir el contacto entre la droga y el órgano efector. Se utilizó el fundus gástrico aislado de rata como órgano efector. La actividad contráctil se registró mediante transductores fuerza-desplazamiento. La muestra I presentó una potencia relativa de 164,0 % con respecto a la II, con límites de confianza de 111,2 y 247,8 % (p=0,95; N=7, y mostró un efecto más prolongado en relación con la II. En las tiras contraídas por la muestra I debió transcurrir 6 min y 46 s, luego del lavado, para que la contracción se redujera a la mitad de la respuesta máxima observada (N=7, en tanto que en las tiras contraídas por la II fue de 1 min y 48 s (N= 8.The contractil potency of a sample of methylic ester of 15 methyl prostaglandin F2 alpha (methyl carboprost (I produced by the Cuban Pharmaceutical Industry, was compared to a commercial sample of prostaglandin F2 alpha (II from a Chinoin firm through the "three point" assay. The duration of the contraction induced by such samples was also compared using the analysis of linear regression of the relaxation observed in function of time after the supression of contact between the drug and the effector organ. The gastric fundus isolated from a rat was used as the effector organ. The contractil activity was recorded by strength-displacement transducers. Sample I showed a relative potency of 164.0 % with respect to sample II, with confidence limits of 111.2 and 247.8 % (P = 0.95; N=7 and it also showed a more prolonged

The rapid-onset dystonia parkinsonism mutation D923N of the Na+, K+-ATPase alpha3 isoform disrupts Na+ interaction at the third Na+ site.

Science.gov (United States)

Einholm, Anja Pernille; Toustrup-Jensen, Mads S; Holm, Rikke; Andersen, Jens Peter; Vilsen, Bente

2010-08-20

Rapid-onset dystonia parkinsonism (RDP), a rare neurological disorder, is caused by mutation of the neuron-specific alpha3-isoform of Na(+), K(+)-ATPase. Here, we present the functional consequences of RDP mutation D923N. Relative to the wild type, the mutant exhibits a remarkable approximately 200-fold reduction of Na(+) affinity for activation of phosphorylation from ATP, reflecting a defective interaction of the E(1) form with intracellular Na(+). This is the largest effect on Na(+) affinity reported so far for any Na(+), K(+)-ATPase mutant. D923N also affects the interaction with extracellular Na(+) normally driving the E(1)P to E(2)P conformational transition backward. However, no impairment of K(+) binding was observed for D923N, leading to the conclusion that Asp(923) is specifically associated with the third Na(+) site that is selective toward Na(+). The crystal structure of the Na(+), K(+)-ATPase in E(2) form shows that Asp(923) is located in the cytoplasmic half of transmembrane helix M8 inside a putative transport channel, which is lined by residues from the transmembrane helices M5, M7, M8, and M10 and capped by the C terminus, recently found involved in recognition of the third Na(+) ion. Structural modeling of the E(1) form of Na(+), K(+)-ATPase based on the Ca(2+)-ATPase crystal structure is consistent with the hypothesis that Asp(923) contributes to a site binding the third Na(+) ion. These results in conjunction with our previous findings with other RDP mutants suggest that a selective defect in the handling of Na(+) may be a general feature of the RDP disorder.

Recikliranje plastenk na kreativen način

OpenAIRE

Pavlin, Suzana

2017-01-01

Plastenke, poleg ostalih plastičnih izdelkov, v zadnjih letih predstavljajo pravo katastrofo za okolje. Domnevamo, da se le redko kdo vpraša, kaj se zgodi z njo po tem, ko je iz nje zaužil še zadnjo kapljico vode. Plastenka je lahko kot večina ostalih komunalnih odpadkov ponovno uporabljena, reciklirana, sežgana ali odložena na deponijo. V primeru nespoštovanja Zakona o varstvu okolja pa pristane v naravi, pogosto v morju, kjer prav počasi razpada in negativno vpliva na celoten ekosistem. V d...

Plant Defensins NaD1 and NaD2 Induce Different Stress Response Pathways in Fungi

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Peter M. Dracatos

2016-09-01

Full Text Available Nicotiana alata defensins 1 and 2 (NaD1 and NaD2 are plant defensins from the ornamental tobacco that have antifungal activity against a variety of fungal pathogens. Some plant defensins interact with fungal cell wall O-glycosylated proteins. Therefore, we investigated if this was the case for NaD1 and NaD2, by assessing the sensitivity of the three Aspergillus nidulans (An O-mannosyltransferase (pmt knockout (KO mutants (An∆pmtA, An∆pmtB, and An∆pmtC. An∆pmtA was resistant to both defensins, while An∆pmtC was resistant to NaD2 only, suggesting NaD1 and NaD2 are unlikely to have a general interaction with O-linked side chains. Further evidence of this difference in the antifungal mechanism was provided by the dissimilarity of the NaD1 and NaD2 sensitivities of the Fusarium oxysporum f. sp. lycopersici (Fol signalling knockout mutants from the cell wall integrity (CWI and high osmolarity glycerol (HOG mitogen-activated protein kinase (MAPK pathways. HOG pathway mutants were sensitive to both NaD1 and NaD2, while CWI pathway mutants only displayed sensitivity to NaD2.

Glutamate Water Gates in the Ion Binding Pocket of Na(+) Bound Na(+), K(+)-ATPase

DEFF Research Database (Denmark)

Han, Minwoo; Kopec, Wojciech; Solov'yov, Ilia A

2017-01-01

III is always protonated. Glutamic acid residues in the three binding sites act as water gates, and their deprotonation triggers water entry to the binding sites. From DFT calculations of Na(+) binding energies, we conclude that three protons in the binding site are needed to effectively bind Na......The dynamically changing protonation states of the six acidic amino acid residues in the ion binding pocket of the Na(+), K(+) -ATPase (NKA) during the ion transport cycle are proposed to drive ion binding, release and possibly determine Na(+) or K(+) selectivity. We use molecular dynamics (MD......(+) from water and four are needed to release them in the next step. Protonation of Asp926 in site III will induce Na(+) release, and Glu327, Glu954 and Glu779 are all likely to be protonated in the Na(+) bound occluded conformation. Our data provides key insights into the role of protons in the Na...

NA62 and NA48/2 results on search for Heavy Neutral Leptons

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Lamanna Gianluca

2018-01-01

Full Text Available In this paper we present new results on upper limits for the search of Heavy Neutral Leptons (HNL with data collected by NA48/2 (2003-2004, NA62-RK (2007 and NA62 (2015 CERN experiments. The data collected with different trigger configuration allow to search for both long and short living heavy neutrinos in the mass range below the kaon mass. In addition the status of the search for K+ → π+vv with the NA62 detector will be briefly presented.

Avaliação química e sensorial da farinha de resíduo de tilápias na forma de sopa Chemical and sensorial evaluation of fish meal in soup

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Flávia Braidotti Stevanato

2007-09-01

Full Text Available As cabeças de tilápias são resíduos do processamento de peixes comumente descartados e não aproveitadas como alimento. Desta forma realizou-se um estudo sobre a composição química e de ácidos graxos na farinha obtida a partir de cabeças de tilápias. Ainda foram realizadas avaliações sensorial (caldo e sopa e química e de ácidos graxos na sopa elaborada com a farinha. Os objetivos foram avaliar a composição e aceitação dos produtos elaborados com farinha, visando o aproveitamento na alimentação humana, especialmente para a merenda escolar. Os resultados obtidos foram de elevados teores de proteína (38,4%, cinzas (19,4% e lipídios (35,5% na farinha. A sopa apresentou elevada aceitação pelas crianças do ensino fundamental e a composição de ácidos graxos do conteúdo lipídico indicou a presença de diversos ácidos ômega-3, especialmente os ácidos alfa-linolênico (LNA, eicosapentaenóico (EPA, docosahexaenócio (DHA e excelente razão AGPI/AGS. Todos estes parâmetros evidenciam que a inclusão de cabeça de tilápia na forma de farinha é aceitável como alimento e constitui uma fonte nutritiva e benéfica para a saúde humana.Fish heads are normally discarded during fish processing and are not used as food. This study therefore investigated the chemical and fatty acids composition of fish meal produced from tilapia heads. An evaluation was made of the sensorial, chemical and fatty acids characteristics of broth and soup containing this fish meal. The purpose of the study was to analyze the composition and acceptance of products containing fish meal, aiming at its use in human food, especially in school meals. The results indicated that the fish meal contained contents of protein (38.4%, ashes (19.4% and lipids (35.5%. The soup containing fish meal was well accepted by elementary schoolchildren. Moreover, the fatty acids composition of the lipid content indicated the presence of several omega-3 acids, especially

Transmural gradients in Na/K pump activity and [Na+]I in canine ventricle.

Science.gov (United States)

Gao, J; Wang, W; Cohen, I S; Mathias, R T

2005-09-01

There are well-documented differences in ion channel activity and action potential shape between epicardial (EPI), midmyocardial (MID), and endocardial (ENDO) ventricular myocytes. The purpose of this study was to determine if differences exist in Na/K pump activity. The whole cell patch-clamp was used to measure Na/K pump current (I(P)) and inward background Na(+)-current (I(inb)) in cells isolated from canine left ventricle. All currents were normalized to membrane capacitance. I(P) was measured as the current blocked by a saturating concentration of dihydro-ouabain. [Na(+)](i) was measured using SBFI-AM. I(P)(ENDO) (0.34 +/- 0.04 pA/pF, n = 17) was smaller than I(P)(EPI) (0.68 +/- 0.09 pA/pF, n = 38); the ratio was 0.50 with I(P)(MID) being intermediate (0.53 +/- 0.13 pA/pF, n = 19). The dependence of I(P) on [Na(+)](i) or voltage was essentially identical in EPI and ENDO (half-maximal activation at 9-10 mM [Na(+)](i) or approximately -90 mV). Increasing [K(+)](o) from 5.4 to 15 mM caused both I(P)(ENDO) and I(P)(EPI) to increase, but the ratio remained approximately 0.5. I(inb) in EPI and ENDO were nearly identical ( approximately 0.6 pA/pF). Physiological [Na(+)](i) was lower in EPI (7 +/- 2 mM, n = 31) than ENDO (12 +/- 3 mM, n = 29), with MID being intermediate (9 +/- 3 mM, n = 22). When cells were paced at 2 Hz, [Na(+)](i) increased but the differences persisted (ENDO 14 +/- 3 mM, n = 10; EPI 9 +/- 2 mM, n = 10; and MID intermediate, 11 +/- 2 mM, n = 9). Based on these results, the larger I(P) in EPI appears to reflect a higher maximum turnover rate, which implies either a larger number of active pumps or a higher turnover rate per pump protein. The transmural gradient in [Na(+)](i) means physiological I(P) is approximately uniform across the ventricular wall, whereas transporters that utilize the transmembrane electrochemical gradient for Na(+), such as Na/Ca exchange, have a larger driving force in EPI than ENDO.

Reactions of metal oxides with molten NaPO3 + NaCl mixtures

International Nuclear Information System (INIS)

Kovarskaya, E.N.; Mityakhina, V.S.; Rodionov, Yu.I.; Silin, M.Yu.

1988-01-01

We consider the dissolution mechanism for iron (III), europium(III), and tin(IV) oxides in molten NaPO 3 + NaCl that are responsible for the peak solubilities. We chose Fe 2 O 3 as the basic material since this occurs in large amounts around damaged metal structures in rock salt mines in a proposed zone for storing vitrified radioactive wastes. Solubility measurement and paper chromatography show that Fe 2 O 3 dissolves in molten NaPO 3 + NaCl in air by reaction with the solvent to give double iron and sodium diphosphates and monophosphates in accordance with the initial solution-in-the-melt composition, the degree of equilibration, and the temperature. The elevated solubilities for initial NaCl contents close to 30 mole % are due to sodium triphosphates and tricyclophosphates present in these melts. Moessbauer spectroscopy confirms that double iron, europium and tin diphosphates and monophosphates containing sodium occur in these chloride-polyphosphate melts

Funciones de la proteína priónica celular, alfa-sinucleína y reelina en enfermedades neurodegenerativas

OpenAIRE

Urrea Zazurca, Laura

2018-01-01

Las enfermedades neurodegenerativas son una serie de trastornos del sistema nervioso caracterizadas por la pérdida de grupos neuronales específicos y por la presencia de cuerpos de inclusión proteicos, entre ellas las más frecuentes son la enfermedad de Alzheimer y la enfermedad de Parkinson, ambas asociadas a la edad. Su etiología, en la mayoría de los casos, aún se desconoce y su manifestación clínica es progresiva y crónica. La enfermedad de Parkinson se caracteriza por la pérdida de ne...

Na(+),K (+)-ATPase as a docking station: protein-protein complexes of the Na(+),K (+)-ATPase.

Science.gov (United States)

Reinhard, Linda; Tidow, Henning; Clausen, Michael J; Nissen, Poul

2013-01-01

The Na(+),K(+)-ATPase, or sodium pump, is well known for its role in ion transport across the plasma membrane of animal cells. It carries out the transport of Na(+) ions out of the cell and of K(+) ions into the cell and thus maintains electrolyte and fluid balance. In addition to the fundamental ion-pumping function of the Na(+),K(+)-ATPase, recent work has suggested additional roles for Na(+),K(+)-ATPase in signal transduction and biomembrane structure. Several signaling pathways have been found to involve Na(+),K(+)-ATPase, which serves as a docking station for a fast-growing number of protein interaction partners. In this review, we focus on Na(+),K(+)-ATPase as a signal transducer, but also briefly discuss other Na(+),K(+)-ATPase protein-protein interactions, providing a comprehensive overview of the diverse signaling functions ascribed to this well-known enzyme.

Characterization of Na+-linked and Na+-independent Cl-/HCO3- exchange systems in Chinese hamster lung fibroblasts

International Nuclear Information System (INIS)

Cassel, D.; Scharf, O.; Rotman, M.; Cragoe, E.J. Jr.; Katz, M.

1988-01-01

The PS120 variant of Chinese hamster lung fibroblasts which lacks Na + /H + exchange activity was used to investigate bicarbonate transport systems and their role in intracellular pH (pH/sub i/) regulation. When pH/sub i/ was decreased by acid load, bicarbonate caused pH/sub i/ increase and stimulated 36 Cl - efflux from the cells, both in a Na + -dependent manner. These results together with previous findings that bicarbonate stimulates 22 Na + uptake in PS120 cells demonstrate the presence of a Na + -linked Cl - /HCO 3 - exchange system. In cells with normal initial pH/sub i/, bicarbonate caused Na + -independent pH/sub i/ increase in Cl - -free solutions and stimulated Na + -independent 36 Cl - efflux, indicating that a Na + -independent Cl - /HCO 3 - exchanger is also present in the cell. Na + -linked and Na + -independent Cl - /HCO 3- exchange is apparently mediated by two distinct systems, since a [(tetrahydrofluorene-7-yl)oxy]acetic acid derivative selectively inhibits the Na + -independent exchanger. An additional distinctive features is a 10-fold lower affinity for chloride of the Na + -linked exchanger. The Na + -linked and Na + -independent Cl - /HCO 3 - exchange systems are likely to protect the cell from acid and alkaline load, respectively

Expression of interferon regulatory factor 4 in chronic myeloid leukemia: correlation with response to interferon alfa therapy.

Science.gov (United States)

Schmidt, M; Hochhaus, A; König-Merediz, S A; Brendel, C; Proba, J; Hoppe, G J; Wittig, B; Ehninger, G; Hehlmann, R; Neubauer, A

2000-10-01

Mice experiments have established an important role for interferon regulatory factor (IRF) family members in hematopoiesis. We wanted to study the expression of interferon regulatory factor 4 (IRF4) in various hematologic disorders, especially chronic myeloid leukemia (CML), and its association with response to interferon alfa (IFN-alpha) treatment in CML. Blood samples from various hematopoietic cell lines, different leukemia patients (70 CML, 29 acute myeloid leukemia [AML], 10 chronic myelomonocytic leukemia [CMMoL], 10 acute lymphoblastic leukemia, and 10 chronic lymphoid leukemia patients), and 33 healthy volunteers were monitored for IRF4 expression by reverse transcriptase polymerase chain reaction. Then, with a focus on CML, the IRF4 level was determined in sorted cell subpopulations from CML patients and healthy volunteers and in in vitro-stimulated CML cells. Furthermore, IRF4 expression was compared in the CML samples taken before IFN-alpha therapy and in 47 additional CML samples taken during IFN-alpha therapy. IRF4 expression was then correlated with cytogenetic response to IFN-alpha. IRF4 expression was significantly impaired in CML, AML, and CMMoL samples. The downregulation of IRF4 in CML samples was predominantly found in T cells. In CML patients during IFN-alpha therapy, a significant increase in IRF4 levels was detected, and this was also observed in sorted T cells from CML patients. The increase seen during IFN-alpha therapy was not due to different blood counts. In regard to the cytogenetic response with IFN-alpha, a good response was associated with high IRF4 expression. IRF4 expression is downregulated in T cells of CML patients, and its increase is associated with a good response to IFN-alpha therapy. These data suggest IRF4 expression as a useful marker to monitor, if not predict, response to IFN-alpha in CML.

Effect of treatment with Omega-3 fatty acids on C-reactive protein and tumor necrosis factor-alfa in hemodialysis patients

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Hamid Tayyebi-Khosroshahi

2012-01-01

Full Text Available C-reactive protein (CRP, a strong independent risk marker of cardiovascular disease (CVD, and tumor necrosis factor-alfa (TNF-α, a known pro-inflammatory cytokine, are elevated and have damaging effects in patients with chronic renal failure (CRF. Omega-3 fatty acids play an important modulatory role in inflammatory responses. The aim of this study is to review the alterations in serum levels of TNF-α, CRP and other parameters caused by omega-3 supplementation in dialysis patients. The clinical trial was performed in 37 patients with end-stage renal disease undergoing dialysis in hemodialysis centers of three university hospitals in Tabriz. Blood samples were obtained from the study patients for hemoglobin, albumin, ferritin, triglyceride, total cholesterol, low-density lipoprotein (LDL and high-density lipoprotein (HDL-cholesterol, TNF-α and high specific-CRP (hs-CRP measurement. The patients received 3 g omega-3 per day for 2 months. The side-effects noticed were nausea, diarrhea and dyspepsia and undesired drug smell. The difference noted in hemoglobin, albumin, ferritin, CRP, triglyceride, total, LDL and HDL-cholesterol before and after supplementation with omega-3 fatty acid was not statistically significant (P > 0.05. However, the use of omega-3 decreased the serum levels of TNF-α significantly. We conclude that the use of 3 g of omega-3 per day caused significant decrease in serum levels of TNF-α in the dialysis population, and its use is recommended in such patients.

Comparação entre a relação PCR/albumina e o índice prognóstico inflamatório nutricional (IPIN Comparison of PCR/albumin ratio with prognostic inflammatory nutritional index (PINI

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Camila Renata Corrêa

2002-07-01

Full Text Available Objetivo: Simplificar o cálculo do índice prognóstico inflamatório nutricional (IPIN empregando número menor de variáveis com conseqüente redução do custo da análise. Materiais e métodos: Foram estudados 54 pacientes e 12 indivíduos-controle com 48 ± 20 (média ± dp anos de idade. As principais patologias dos pacientes eram: doença arterial periférica (22, pênfigo foliáceo (7, doença inflamatória intestinal (7, trauma (6 e pós-operatório de ortognatia (3. Foram obtidas amostras de sangue periférico, colhidas em jejum para dosagens de proteínas positivas (+ e negativas (- de fase aguda (PFA pelo método nefelométrico. Proteína C reativa (PCR, alfa-1-glicoproteína-ácida (alfa-1-GA, alfa-1-antitripsina (alfa-1-AT e ceruloplasmina (CER foram as PFA+ e albumina (Alb, transtiretina (TTR, transferrina (TF e proteína ligadora do retinol (RBP foram as representantes das PFA-. Esses valores foram analisados quanto à associação de correlação isolada ou associadamente na fórmula do índice prognóstico inflamatório e nutricional (IPIN = PCR + alfa-1-GA / Alb + TTR. De acordo com o índice prognóstico inflamatório e nutricional, os pacientes foram classificados em grupo-controle (G1; pacientes sem infecção/inflamação (IPIN 1, G3. Em seguida os pacientes do G3 foram subdivididos em baixo risco (G3A, n = 16; médio risco ( G3B, n = 10; alto risco (G3C, n = 6 e com risco de morte (G3D, n = 11. Os resultados foram correlacionados entre si (teste de Spearman ou submetidos às comparações entre grupos (teste de Kruskall-Wallis. Resultados: Houve relação significativa entre as variáveis PCR ´ alfa-1-GA (r = 0,49, Alb ´ TTR (r = 0,60, Alb ´ RBP (r = 0,58, Alb ´ TF (r = 0,39, TTR ´ RBP (r = 0,56 e TTR´ TF (r = 0,43 e as melhores relações encontradas entre PFA+ e PFA- foram: PCR ´ Alb (r = - 0,71, PCR ´ TTR (r = - 0,54, PCR ´ TF (r = - 0,39 e alfa-1-GA ´ Alb (r = - 0,35. Os valores do IPIN mostraram a

Na/K Pump and Beyond: Na/K-ATPase as a Modulator of Apoptosis and Autophagy

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Cassiano Felippe Gonçalves-de-Albuquerque

2017-04-01

Full Text Available Lung cancer is a leading cause of global cancer deaths. Na/K-ATPase has been studied as a target for cancer treatment. Cardiotonic steroids (CS trigger intracellular signalling upon binding to Na/K-ATPase. Normal lung and tumour cells frequently express different pump isoforms. Thus, Na/K-ATPase is a powerful target for lung cancer treatment. Drugs targeting Na/K-ATPase may induce apoptosis and autophagy in transformed cells. We argue that Na/K-ATPase has a role as a potential target in chemotherapy in lung cancer treatment. We discuss the effects of Na/K-ATPase ligands and molecular pathways inducing deleterious effects on lung cancer cells, especially those leading to apoptosis and autophagy.

Na/K Pump and Beyond: Na/K-ATPase as a Modulator of Apoptosis and Autophagy.

Science.gov (United States)

Felippe Gonçalves-de-Albuquerque, Cassiano; Ribeiro Silva, Adriana; Ignácio da Silva, Camila; Caire Castro-Faria-Neto, Hugo; Burth, Patrícia

2017-04-21

Lung cancer is a leading cause of global cancer deaths. Na/K-ATPase has been studied as a target for cancer treatment. Cardiotonic steroids (CS) trigger intracellular signalling upon binding to Na/K-ATPase. Normal lung and tumour cells frequently express different pump isoforms. Thus, Na/K-ATPase is a powerful target for lung cancer treatment. Drugs targeting Na/K-ATPase may induce apoptosis and autophagy in transformed cells. We argue that Na/K-ATPase has a role as a potential target in chemotherapy in lung cancer treatment. We discuss the effects of Na/K-ATPase ligands and molecular pathways inducing deleterious effects on lung cancer cells, especially those leading to apoptosis and autophagy.

Subnitride chemistry: A first-principles study of the NaBa3N, Na5Ba3N, and Na16Ba6N phases

International Nuclear Information System (INIS)

Oliva, Josep M.

2005-01-01

An ab initio study on the electronic structure of the subnitrides NaBa 3 N, Na 5 Ba 3 N, and Na 16 Ba 6 N is performed for the first time. The NaBa 3 N and Na 5 Ba 3 N phases consist of infinite 1 ∞ [NBa 6/2 ] strands composed of face-sharing NBa 6 octahedra surrounded by a 'sea' of sodium atoms. The Na 16 Ba 6 N phase consist of discrete [NBa 6 ] octahedra arranged in a body-cubic fashion, surrounded by a 'sea' of sodium atoms. Our calculations suggest that the title subnitrides are metals. Analysis of the electronic structure shows partial interaction of N(2s) with Ba(5p) electrons in the lower energy region for NaBa 3 N and Na 5 Ba 3 N. However, no dispersion is observed for the N(2s) and Ba(5p) bands in the cubic phase Na 16 Ba 6 N. The metallic band below the Fermi level shows a strong mixing of N(2p), Ba(6s), Ba(5d), Ba(6p), Na(3s) and Na(3p) orbitals. The metallic character in these nitrides stems from delocalized electrons corresponding to hybridized 5d l 6s m 6p n barium orbitals which interact with hybridized 3s n 3p m sodium orbitals. Analysis of the electron density and electronic structure in these nitrides shows two different regions: a metallic matrix corresponding to the sodium atoms and the regions around them and heteropolar bonding between nitrogen and barium within the infinite 1 ∞ [NBa 6/2 ] strands of the NaBa 3 N and Na 5 Ba 3 N phases, and within the isolated [NBa 6 ] octahedra of the Na 16 Ba 6 N phase. The nitrogen atoms inside the strands and octahedra are negatively charged, the anionic character of nitrogens being larger in the isolated octahedra of the cubic phase Na 16 Ba 6 N, due to the lack of electron delocalization along one direction as opposed to the other phases. The sodium and barium atoms appear to be slightly negatively and positively charged, the latter to a larger extent. From the computed Ba-N overlap populations as well as the analysis of the contour maps of differences between total density and superposition of

Determinación por valoración ácido-base de serina en una mezcla de aminoácidos. Problema interactivo.

OpenAIRE

Milla González, Miguel

2013-01-01

Las alfa-hidroxilaminas son capaces de reaccionar con peryodato liberando amoníaco. En este problema interactivo se hace uso de esta propiedad para encontrar la concentración del aminoácido serina en presencia de glicina y alanina. El amoníaco producido se recoge sobre HCl y el exceso de éste se valora con NaOH. Los datos del ejercicio son aleatorios y para resolverlo es necesario montar a modo de puzzle la expresión de conversión de la valoración por retroceso, incluyendo las magnitudes dime...

WPŁYW WIEKU NA WYTRZYMAŁOŚĆ NA ŚCISKANIE BETONU MODYFIKOWANEGO DODATKIEM METAKAOLINITU

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Janusz KONKOL

Full Text Available W artykule przedstawiono wyniki badań wytrzymałości na ściskanie betonów modyfikowanych dodatkiem metakaolinitu po 2, 3, 7, 14, 28 i 56 dniach dojrzewania. Dodatek metakaolinitu użyto jako częściowy substytut cementu, dokonując wymiany 10% masy cementu na metakaolinit. Udział ten uznawany jest za optymalny z uwagi na inne niż wytrzymałość na ściskanie właściwości betonu, w tym trwałość betonu modyfikowanego metakaolinitem. Badania przeprowadzono także dla betonu tła, betonu bez dodatku metakaolinitu. Betony wykonano przy założeniu stałego stosunku woda/spoiwo, względnie woda/cement wynoszącego 0,45 oraz przy użyciu cementu portlandzkiego CEM I 32,5R, piasku kwarcowego frakcji do 2 mm oraz grysu bazaltowego frakcji do 16 mm. W celu uzyskania pożądanej konsystencji mieszanki betonowej zastosowano upłynniacz na bazie estrów polikarboksylowych. Uzyskane rezultaty badań potwierdziły wysoką aktywność pucolanową metakaolinitu już we wczesnym okresie dojrzewania betonu. W okresie między 3 a 7 dniem dojrzewania stwierdzono wolniejszy przyrost wytrzymałości na ściskanie w betonie niemodyfikowanym metakaolinitem, podczas gdy w betonie, w którym 10% masy cementu zastąpiono metakaolinitem uwidocznił się znaczący przyrost wytrzymałości na ściskanie. Po 2 dniach dojrzewania wytrzymałość na ściskanie betonu tła była nieznacznie wyższa od wytrzymałości na ściskanie betonu modyfikowanego. Korzystny wpływ dodatku metakaolinitu wynikający z jego wysokiej aktywności pucolanowej, jak również z uszczelniającego charakteru tego dodatku obserwowano jest zwłaszcza w okresie między 3 a 14 dniem dojrzewania. W tym okresie obserwowano znaczny przyrost wytrzymałości na ściskanie betonu modyfikowanego w porównaniu z wytrzymałością na ściskanie betonu tła. Po 7 i 14 dniach dojrzewania stwierdzono odpowiednio prawie 25% i 21% wzrost wytrzymałości na ściskanie na skutek użycia metakaolinitu.

Teratological studies of DTPA-CaNa3, DTPA-ZnNa3 and quinamic acid in mice

International Nuclear Information System (INIS)

Luo Meichu; Ruan Tianming; Tong Shungao

1989-01-01

DTPA-CaNa 3 , DTPA-ZnNa 3 and quinamic acid are effective chelating agents for removing actinide elements from the body. In this experiment, different doses of DTPA-CaNa 3 , DTPA-ZnNa 3 and quinamic acid were given to mice on gestation days 6-10. Eight groups of mice received 0.8 and 2.0 mM/kg of DTPA-CaNa 3 , 3.8, 7.6, and 11.4 mM/kg of DTPA-ZnNa 3 and 0.42, 2.1, and 4.2 mM/kg of quinamic acid. Hypetonic saline and isotonic saline were given to two control groups. DTPA-CaNa 3 and quinamidic acid were found to be much more toxic to fetus of mice than DTPA-ZnNa 3 . When the doses of DTPA-CaMa 3 and quinamidic acid were 20 times higher than the human dose, the number of resorbed fetus was increased and the number and weight of live fetus were reduced. The result of injection with 7.6 mM/kg (200 times of human dose)DTPA-ZnNa 3 and that of injection with isotonic saline are the same. Therefore, we suggest that the DTPA-CaNa 3 and quinamidic acid should not be given to pregnant woman, if chelation therapy is needed, while the much safer DTPA-ZnNa 3 could be used

Na-ion dynamics in Quasi-1D compound NaV2O4

International Nuclear Information System (INIS)

Månsson, M; Umegaki, I; Nozaki, H; Higuchi, Y; Sugiyama, J; Kawasaki, I; Watanabe, I; Sakurai, H

2014-01-01

We have used the pulsed muon source at ISIS to study high-temperature Na-ion dynamics in the quasi-one-dimensional (Q1D) metallic antiferromagnet NaV 2 O 4 . By performing systematic zero-field and longitudinal-field measurements as a function of temperature we clearly distinguish that the hopping rate increases exponentially above T diff ≈ 250 K. The data is well fitted to an Arrhenius type equation typical for a diffusion process, showing that the Na-ions starts to be mobile above T diff . Such results make this compound very interesting for the tuning of Q1D magnetism using atomic-scale ion-texturing through the periodic potential from ordered Na-vacancies. Further, it also opens the door to possible use of NaV 2 O 4 and related compounds in energy related applications

O Passeio Público do Rio de Janeiro na Literatura, na Pintura e na Fotografia do Século XIX

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Solange de Aragão

2012-06-01

Full Text Available Este artigo tem como objetivo geral e mais amplo chamar a atenção dos pesquisadores para a importância da literatura, da pintura e da fotografia como fontes documentais para a construção da História do Paisagismo no Brasil por meio de um estudo de caso muito particular: o Passeio Público do Rio de Janeiro no século XIX. São objetivos específicos apresentar e analisar o modo como esse espaço livre público aparece na literatura, na pintura e na fotografia desse período, considerando suas transformações paisagísticas.

Solubility of NaNd(CO3)2.6H2O(c) in concentrated Na2CO3 and NaHCO3 solutions

International Nuclear Information System (INIS)

Rao, L.; Rai, D.; Felmy, A.R.; Fulton, R.W.; Novak, C.F.

1996-01-01

NaNd(CO 3 ) 2 x 6 H 2 O(c) was identified to be the final equilibrium solid phase in suspensions containing concentrated sodium carbonate (0.1 to 2.0 M) and sodium bicarbonate (0.1 to 1.0 M), with either NaNd(CO 3 ) 2 x 6 H 2 O(c) or Nd 2 (CO 3 ) 3 x xH 2 O(s) as initial solids. A thermodynamic model, based on Pitzer's specific into-interaction approach, was developed to interpret the solubility of NaNd(CO 3 ) 2 x 6 H 2 O(c) as functions of sodium carbonate and sodium bicarbonate concentrations. In this model, the solubility data of NaNd(CO 3 ) 2 x 6 H 2 O(c) were explained by assuming the formation of NdCO 3 + , Nd(CO 3 ) 2 - and Nd(CO 3 ) 3 3- species and invoking the specific ion interactions between Na + and Nd(CO 3 ) 3 3- . Ion interaction parameters for Na + -Nd(CO 3 ) 3 3- were developed to fit the solubility data. Based on the model calculations, Nd(CO 3 ) 3 3- was the predominant aqueous neodymium species in 0.1 to 2 M sodium carbonate and 0.1 to 1 M sodium bicarbonate solutions. The logarithm of the NaNd(CO 3 ) 2 x 6 H 2 O solubility product (NaNd(CO 3 ) 2 x 6 H 2 O(c)=Na + +Nd 3+ +2 CO 3 2- +6 H 2 O) was calculated to be -21.39. This model also provided satisfactory interpretation of the solubility data of the analogous Am(III) system in less concentrated carbonate and bicarbonate solutions. (orig.)

Proteômica na sepse: estudo piloto Proteomics in sepsis: a pilot study

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Rita Azevedo de Paiva

2010-12-01

Full Text Available Na sepse ocorre desregulação da expressão gênica. Os marcadores genéticos revelam apenas o genótipo do indivíduo, não sendo afetados pelos processos biológicos decorrentes da ação do ambiente, estes expressos nas proteínas. Este estudo teve como objetivo alcançar maior compreensão sobre as bases moleculares da sepse. Para tal realizou a identificação e análise da expressão diferencial de proteínas no soro de paciente séptico em diferentes estágios de gravidade (sepse, sepse grave e choque séptico através de técnicas proteômicas. Amostras de soro referentes a cada estágio da sepse foram colhidas e submetidas à eletroforese unidimensional em fitas com gradiente de pH imobilizado seguida de eletroforese bidimensional em gel de poliacrilamida 12,5%. Os géis obtidos foram corados, escaneados e analisados através do programa ImageMasterPlatinum. As proteínas expressas diferencialmente nos géis foram excisadas, digeridas com tripsina e identificadas através de espectrometria de massa. Foram identificadas 14 proteínas expressas diferencialmente entre os estágios da sepse, assim como uma proteína não expressa em todos os estágios, sugerindo a existência de um possível biomarcador. Foram elas: amilóide sérico A, apolipoproteína A-1 (2 isoformas, proteína dedo de zinco 222, albumina humana, PRO 2619, imunoglobulina de cadeia leve kappa região VLJ, imunoglobulina M monoclonal de aglutinação a frio e 7 inibidoras de proteases - alfa-1 antitripsina. Os resultados obtidos neste estudo piloto demonstram a participação das vias do complemento e coagulação, do metabolismo lipídico e da informação genética na sepse. A grande maioria de proteínas identificadas está envolvida no sistema imune com predomínio das proteínas inibidoras de proteases.Gene expression is disrupted by sepsis. Genetic markers can only reveal a patient's genotype, and they are not affected by environmental biological processes. These

Mechanism of μ-conotoxin PIIIA binding to the voltage-gated Na+ channel NaV1.4.

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Rong Chen

Full Text Available Several subtypes of voltage-gated Na+ (NaV channels are important targets for pain management. μ-Conotoxins isolated from venoms of cone snails are potent and specific blockers of different NaV channel isoforms. The inhibitory effect of μ-conotoxins on NaV channels has been examined extensively, but the mechanism of toxin specificity has not been understood in detail. Here the known structure of μ-conotoxin PIIIA and a model of the skeletal muscle channel NaV1.4 are used to elucidate elements that contribute to the structural basis of μ-conotoxin binding and specificity. The model of NaV1.4 is constructed based on the crystal structure of the bacterial NaV channel, NaVAb. Six different binding modes, in which the side chain of each of the basic residues carried by the toxin protrudes into the selectivity filter of NaV1.4, are examined in atomic detail using molecular dynamics simulations with explicit solvent. The dissociation constants (Kd computed for two selected binding modes in which Lys9 or Arg14 from the toxin protrudes into the filter of the channel are within 2 fold; both values in close proximity to those determined from dose response data for the block of NaV currents. To explore the mechanism of PIIIA specificity, a double mutant of NaV1.4 mimicking NaV channels resistant to μ-conotoxins and tetrodotoxin is constructed and the binding of PIIIA to this mutant channel examined. The double mutation causes the affinity of PIIIA to reduce by two orders of magnitude.

Valores de referência do tempo de protrombina (TP e tempo de tromboplastina parcial ativada (TTPa em cães Reference ranges of prothrombin time (PT and activated partial thromboplastin time (aPTT in dogs

Directory of Open Access Journals (Sweden)

Sonia Terezinha dos Anjos Lopes

2005-04-01

Full Text Available Os fatores de coagulação são parte integrante da hemostasia normal, e tanto as coagulopatias hereditárias como adquiridas que envolvem este sistema são de grande importância veterinária, geralmente evidenciadas por manifestações clínicas, tais como, petéquias, equimoses, hematomas e sangramentos tardios. O presente trabalho teve por objetivo a determinação dos valores de referência do tempo de protrombina (TP e tempo de tromboplastina parcial ativada (TTPa, por métodos manuais. Para tanto, utilizou-se "kits" para dosagens humanas, devido à inexistência de produtos similares de uso específico veterinário. Foram utilizados 40 cães clinicamente sadios, sem raça definida, machos ou fêmeas, de diferentes idades. As amostras sanguíneas foram de 2,5ml cada, colhidas por venopunção cefálica e acondicionadas em tubos de centrífuga contendo 0,25ml de citrato de sódio a 3,8%. O plasma foi imediatamente separado por centrifugação e as determinações de TP e TTPa foram realizadas utilizando-se "kits" comerciais "HemoStat Thromboplastin-SIª" e "HemoStat aPTT-El b", respectivamente. Os resultados obtidos foram de 6,87 ± 1,4 segundos para o TP com valores mínimo e máximo de 4,07 e 9,67, respectivamente, e de 15,10 ± 1,6 segundos para TTPa com valores mínimo e máximo de 11,9 e 18,3, respectivamente. Conclui-se, que os valores obtidos neste trabalho podem ser utilizados como referência. Os reagentes utilizados para plasma humano podem ser empregados para o plasma de cães.The coagulation factors are part of normal hemostasis, and both hereditary and acquired coagulopathies that involve this system have an important role in veterinary medicine, generally evidenced by clinical signs such as: petechias, ecchymosis, hematomas and late hemorrhagies. The objective of this experiment was to determine reference range values of prothrombin time (PT and activated partial thromboplastin time (aPTT, for manual methods. Human commercial

Muscle K+, Na+, and Cl- disturbances and Na+-K+ pump inactivation: implications for fatigue

DEFF Research Database (Denmark)

McKenna, Michael J; Bangsbo, Jens; Renaud, Jean-Marc

2008-01-01

(+)-ATPase activity during exercise stabilizes Na(+) and K(+) concentration gradients and membrane excitability and thus protects against fatigue. However, during intense contraction some Na(+)-K(+) pumps are inactivated and together with further ionic disturbances, likely precipitate muscle fatigue.......Membrane excitability is a critical regulatory step in skeletal muscle contraction and is modulated by local ionic concentrations, conductances, ion transporter activities, temperature, and humoral factors. Intense fatiguing contractions induce cellular K(+) efflux and Na(+) and Cl(-) influx......, causing pronounced perturbations in extracellular (interstitial) and intracellular K(+) and Na(+) concentrations. Muscle interstitial K(+) concentration may increase 1- to 2-fold to 11-13 mM and intracellular K(+) concentration fall by 1.3- to 1.7-fold; interstitial Na(+) concentration may decline by 10 m...

Route, mechanism, and implications of proton import during Na+/K+ exchange by native Na+/K+-ATPase pumps

Science.gov (United States)

Vedovato, Natascia

2014-01-01

A single Na+/K+-ATPase pumps three Na+ outwards and two K+ inwards by alternately exposing ion-binding sites to opposite sides of the membrane in a conformational sequence coupled to pump autophosphorylation from ATP and auto-dephosphorylation. The larger flow of Na+ than K+ generates outward current across the cell membrane. Less well understood is the ability of Na+/K+ pumps to generate an inward current of protons. Originally noted in pumps deprived of external K+ and Na+ ions, as inward current at negative membrane potentials that becomes amplified when external pH is lowered, this proton current is generally viewed as an artifact of those unnatural conditions. We demonstrate here that this inward current also flows at physiological K+ and Na+ concentrations. We show that protons exploit ready reversibility of conformational changes associated with extracellular Na+ release from phosphorylated Na+/K+ pumps. Reversal of a subset of these transitions allows an extracellular proton to bind an acidic side chain and to be subsequently released to the cytoplasm. This back-step of phosphorylated Na+/K+ pumps that enables proton import is not required for completion of the 3 Na+/2 K+ transport cycle. However, the back-step occurs readily during Na+/K+ transport when external K+ ion binding and occlusion are delayed, and it occurs more frequently when lowered extracellular pH raises the probability of protonation of the externally accessible carboxylate side chain. The proton route passes through the Na+-selective binding site III and is distinct from the principal pathway traversed by the majority of transported Na+ and K+ ions that passes through binding site II. The inferred occurrence of Na+/K+ exchange and H+ import during the same conformational cycle of a single molecule identifies the Na+/K+ pump as a hybrid transporter. Whether Na+/K+ pump–mediated proton inflow may have any physiological or pathophysiological significance remains to be clarified. PMID

Route, mechanism, and implications of proton import during Na+/K+ exchange by native Na+/K+-ATPase pumps.

Science.gov (United States)

Vedovato, Natascia; Gadsby, David C

2014-04-01

A single Na(+)/K(+)-ATPase pumps three Na(+) outwards and two K(+) inwards by alternately exposing ion-binding sites to opposite sides of the membrane in a conformational sequence coupled to pump autophosphorylation from ATP and auto-dephosphorylation. The larger flow of Na(+) than K(+) generates outward current across the cell membrane. Less well understood is the ability of Na(+)/K(+) pumps to generate an inward current of protons. Originally noted in pumps deprived of external K(+) and Na(+) ions, as inward current at negative membrane potentials that becomes amplified when external pH is lowered, this proton current is generally viewed as an artifact of those unnatural conditions. We demonstrate here that this inward current also flows at physiological K(+) and Na(+) concentrations. We show that protons exploit ready reversibility of conformational changes associated with extracellular Na(+) release from phosphorylated Na(+)/K(+) pumps. Reversal of a subset of these transitions allows an extracellular proton to bind an acidic side chain and to be subsequently released to the cytoplasm. This back-step of phosphorylated Na(+)/K(+) pumps that enables proton import is not required for completion of the 3 Na(+)/2 K(+) transport cycle. However, the back-step occurs readily during Na(+)/K(+) transport when external K(+) ion binding and occlusion are delayed, and it occurs more frequently when lowered extracellular pH raises the probability of protonation of the externally accessible carboxylate side chain. The proton route passes through the Na(+)-selective binding site III and is distinct from the principal pathway traversed by the majority of transported Na(+) and K(+) ions that passes through binding site II. The inferred occurrence of Na(+)/K(+) exchange and H(+) import during the same conformational cycle of a single molecule identifies the Na(+)/K(+) pump as a hybrid transporter. Whether Na(+)/K(+) pump-mediated proton inflow may have any physiological or

Growth mechanism of NaClO 3 and NaBrO 3 crystals from aqueous ...

Indian Academy of Sciences (India)

A study of growth rates of NaClO3 and NaBrO3 has been carried out using a small growth cell by in situ observation. Normal growth rates of {100} faces of NaClO3 and {111} faces of NaBrO3 along ⟨ 110 ⟩ direction are measured under relatively high supersaturation ranging from 3–8%. In the initial stages of growth, {100}, ...

Rescue of Na+ affinity in aspartate 928 mutants of Na+,K+-ATPase by secondary mutation of glutamate 314.

Science.gov (United States)

Holm, Rikke; Einholm, Anja P; Andersen, Jens P; Vilsen, Bente

2015-04-10

The Na(+),K(+)-ATPase binds Na(+) at three transport sites denoted I, II, and III, of which site III is Na(+)-specific and suggested to be the first occupied in the cooperative binding process activating phosphorylation from ATP. Here we demonstrate that the asparagine substitution of the aspartate associated with site III found in patients with rapid-onset dystonia parkinsonism or alternating hemiplegia of childhood causes a dramatic reduction of Na(+) affinity in the α1-, α2-, and α3-isoforms of Na(+),K(+)-ATPase, whereas other substitutions of this aspartate are much less disruptive. This is likely due to interference by the amide function of the asparagine side chain with Na(+)-coordinating residues in site III. Remarkably, the Na(+) affinity of site III aspartate to asparagine and alanine mutants is rescued by second-site mutation of a glutamate in the extracellular part of the fourth transmembrane helix, distant to site III. This gain-of-function mutation works without recovery of the lost cooperativity and selectivity of Na(+) binding and does not affect the E1-E2 conformational equilibrium or the maximum phosphorylation rate. Hence, the rescue of Na(+) affinity is likely intrinsic to the Na(+) binding pocket, and the underlying mechanism could be a tightening of Na(+) binding at Na(+) site II, possibly via movement of transmembrane helix four. The second-site mutation also improves Na(+),K(+) pump function in intact cells. Rescue of Na(+) affinity and Na(+) and K(+) transport by second-site mutation is unique in the history of Na(+),K(+)-ATPase and points to new possibilities for treatment of neurological patients carrying Na(+),K(+)-ATPase mutations. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

The system NaVO3-Na2WO4-Na2W2O7

International Nuclear Information System (INIS)

Kazanbekov, V.R.; Gasanaliev, A.M.; Kazanbekov, R.G.

1994-01-01

Phase diagrams of sodium metavanadate-sodium ditungstate, sodium metavanadate-sodium tungstate systems and surface of primary crystallization of sodium metavabadate-sodium tungstate-sodium ditungstate system were studied. The system sodium metavanadate-sodium ditungstate is eutectic one. Compound NaVO 3 x2Na 2 WO 4 is formed in solid state in sodium metavanadate-sodium tungstate system. Liquidus surface of sodium metavanadate-sodium tungstate-sodium ditungstate is presented by three crystallization fields of initial components. Composition and melting point of ternary eutectics are determined

Summer sudden Na number density enhancements measured with the ALOMAR Weber Na Lidar

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D. Heinrich

2008-05-01

Full Text Available We present summer Na-densities and atmospheric temperatures measured 80 to 110 km above the Arctic Lidar Observatory for Middle Atmosphere Research (ALOMAR. The Weber Na Lidar is part of ALOMAR, located at 69° N in Norway, 150 km north of the Arctic Circle. The sun does not set here during the summer months, and measurements require a narrowband Faraday Anomalous Dispersion Optical Filter (FADOF.

We discuss an observed sudden enhancement in the Na number density around 22:00 UT on 1 to 2 June 2006. We compare this observation with previous summer measurements and find a frequent appearance of Na number density enhancements near local midnight. We describe the time of appearance, the altitude distribution, the duration and the strength of these enhancements and compare them to winter observations. We investigate possible formation mechanisms and, as others before, we find a strong link between these Na number density enhancements and sporadic E layers.

Summer sudden Na number density enhancements measured with the ALOMAR Weber Na Lidar

Directory of Open Access Journals (Sweden)

D. Heinrich

2008-05-01

Full Text Available We present summer Na-densities and atmospheric temperatures measured 80 to 110 km above the Arctic Lidar Observatory for Middle Atmosphere Research (ALOMAR. The Weber Na Lidar is part of ALOMAR, located at 69° N in Norway, 150 km north of the Arctic Circle. The sun does not set here during the summer months, and measurements require a narrowband Faraday Anomalous Dispersion Optical Filter (FADOF. We discuss an observed sudden enhancement in the Na number density around 22:00 UT on 1 to 2 June 2006. We compare this observation with previous summer measurements and find a frequent appearance of Na number density enhancements near local midnight. We describe the time of appearance, the altitude distribution, the duration and the strength of these enhancements and compare them to winter observations. We investigate possible formation mechanisms and, as others before, we find a strong link between these Na number density enhancements and sporadic E layers.

PERCEPCIÓN DEL IMPACTO DE LA APLICACIÓN DE MECANISMOS DE ASEGURAMIENTO DE LA CALIDAD EN LA EDUCACIÓN SUPERIOR EN COSTA RICA: PROYECTO ALFA-CINDA-UNIVERSIDAD DE COSTA RICA (PERCEIVED IMPACT OF QUALITY ASSURANCE MECHANISMS ON COSTA-RICAN HIGHER EDUCATION: THE ALFA-CINDA-UNIVERSIDAD-DE-COSTA-RICA PROJECT

Directory of Open Access Journals (Sweden)

Guido Guido, Elsiana

2012-05-01

Full Text Available Resumen: En este artículo se presentan las acciones realizadas dentro del Proyecto Alfa Nro. DCl-ALA 2008/42: Aseguramiento de la calidad: políticas públicas y gestión universitaria financiado con fondos de la Unión Europea y coordinado, de manera general, por el Centro Interuniversitario de Desarrollo, CINDA. Participan 16 universidades latinoamericanas y 7 universidades europeas, y el objetivo general del proyecto es mejorar la gestión de la calidad de la Educación Superior, tanto a nivel de los sistemas nacionales como de las instituciones de Educación Superior, y contribuir con la generación de conocimiento y confianzas mutuas acerca de la calidad de la Educación Superior entre los países latinoamericanos y europeos. Como parte de las actividades desarrolladas se llevaron a cabo estudios en 7 países (5 de Latinoamérica y 2 de Europa para evaluar el impacto de los procesos de aseguramiento de la calidad implementados en las universidades. En Costa Rica, el proyecto ha sido coordinado y ejecutado por la Universidad de Costa Rica desde el año 2009 hasta la fecha. El estudio se realizó en dos universidades estatales y dos privadas, durante el período comprendido entre octubre de 2010 y mayo de 2011, se realizaron 26 entrevistas presenciales y 8 sesiones de grupo a las diferentes poblaciones definidas en el estudio, además, se realizaron un total de 652 encuestas a estudiantes activos y a personas egresadas. Los principales resultados obtenidos en Costa Rica se resumen en este artículo, de acuerdo con los niveles y dimensiones de calidad que fueron definidos en el marco referencial del proyecto.Abstract: The article presents actions carried out in Costa Rica, in the frame of the project Alfa Nro. DCl-ALA 2008/42: Quality assurance: public policies and management in universities. This project is funded by the European Commission and its general coordination is in charge of the Inter-university Center for Development (CINDA, in

Muziki wa Hip Hop na Haki Za Kijamii: Dhima, Changamoto na ...

African Journals Online (AJOL)

Ni dhahiri kuwa haki za kijamii zinaweza kuwasilishwa kwa jamii pana kupitia sanaa ya hip hop. Makala haya basi, yanabainisha dhima na mchango wa muziki wa hip hop katika masuala ya haki za kijamii, yanafafanua changamoto za muziki huu katika kuwasilisha haki za kijamii na kutoa mapendekezo kwa makundi ...

Sequential growth of sandwiched NaYF{sub 4}:Yb/Er@NaYF{sub 4}:Yb@NaNdF{sub 4}:Yb core–shell–shell nanoparticles for photodynamic therapy

Energy Technology Data Exchange (ETDEWEB)

Peng, Huang-Yong; Ding, Bin-Bin; Ma, Yin-Chu [Department of Medical Materials and Rehabilitation Engineering, School of Medical Engineering, Hefei University of Technology, Hefei 230009 (China); Sun, Shi-Qi [State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046 (China); Tao, Wei [Department of Medical Materials and Rehabilitation Engineering, School of Medical Engineering, Hefei University of Technology, Hefei 230009 (China); Guo, Yan-Chuan [Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190 (China); Guo, Hui-Chen, E-mail: ghch-2004@hotmail.com [State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046 (China); Yang, Xian-Zhu, E-mail: yangxz@hftu.edu.cn [Department of Medical Materials and Rehabilitation Engineering, School of Medical Engineering, Hefei University of Technology, Hefei 230009 (China); Qian, Hai-Sheng, E-mail: shqian@hfut.edu.cn [Department of Medical Materials and Rehabilitation Engineering, School of Medical Engineering, Hefei University of Technology, Hefei 230009 (China)

2015-12-01

Graphical abstract: The monodisperse elliptical NaYF{sub 4}:Yb/Er@NaYF{sub 4}:Yb@NaNdF{sub 4}:Yb core–shell–shell nanoparticles have been synthesized successfully by a facile sequential growth process, which can be used as transducer for photodynamic therapy of cancer cells. - Highlights: • The NaYF{sub 4}:Yb/Er@NaYF{sub 4}:Yb@NaNdF{sub 4}:Yb nanoparticles have been fabricated successfully. • The as-prepared nanoparticles show strong fluorescence excited at 980 or 808 nm. • The nanoparticles were transferred into the aqueous phase via a facile process. • Photosensitizers were loaded into the composites for photodynamic therapy. - Abstract: Upconversion (UC) nanostructures have attracted much interest for their extensive biological applications. In this work, we describe a sequential synthetic route to prepare sandwiched NaYF{sub 4}:Yb/Er@NaYF{sub 4}:Yb@NaNdF{sub 4}:Yb core–shell upconversion nanoparticles. The as-prepared products were investigated by X-ray diffraction (XRD) and transmission electron microscopy (TEM, JEM 2100F), respectively. The as-prepared core–shell nanoparticles of NaYF{sub 4}:Yb/Er@NaYF{sub 4}:Yb@NaNdF{sub 4}:Yb are composed of elliptical nanoparticles with a length of 80 nm and width of 42 nm, which show efficient upconversion fluorescence excited at 808 nm indicating the formation of core–shell–shell sandwiched nanostructures. In addition, the as-prepared sandwiched NaYF{sub 4}:Yb/Er@NaYF{sub 4}:Yb@NaNdF{sub 4}:Yb core–shell upconversion nanoparticles also show strong upconversion fluorescence excited at 980 nm. Amphiphilic mPEG{sub 2k}-b-PEBEP{sub 6K} copolymers (denoted as PPE) were chosen to transfer these hydrophobic UCNPs into the aqueous phase for biological application. In vitro photodynamic therapy of cancer cells show that the viability of cells incubated with the nanoparticles loaded with MC 540 was significantly lower as compared to the nanoparticles without photosensitizers exposed to NIR laser.

Intracellular Na+ regulation of Na+ pump sites in cultured vascular smooth muscle cells

International Nuclear Information System (INIS)

Allen, J.C.; Navran, S.S.; Seidel, C.L.; Dennison, D.K.; Amann, J.M.; Jemelka, S.K.

1989-01-01

Enzymatically dispersed cells from canine saphenous vein and femoral artery were grown in fetal calf serum and studied at day 0 (freshly dispersed) through confluence in primary culture. Intracellular Na levels (Nai), but not intracellular K (Ki), were increased after 24 h in culture and then decreased to a steady state by 4 days. Na+ pump site number [( 3 H] ouabain binding) increased through day 3 and remained elevated. Nai was still elevated at 2 days when the Na+ pump site number began to increase. Total pump turnover (maximum ouabain-inhibited 86 Rb uptake) reflected the increase in Na+ pump site number. These key events precede the observed increases in both protein production and cellular proliferation. If the same cells are maintained in defined medium, without fetal calf serum, Nai, Ki, and the number of [ 3 H]ouabain binding sites do not change with time. These data are consistent with the suggestion that the initial mitogenic response of vascular smooth muscle cells to fetal calf serum involves an increased Na+ influx, and a Nai accumulation, caused by low Na+ pump density. The synthesis of new pump sites effects a decrease in the accumulated Nai, which may be related to cell proliferation

On the Stability of NaO2 in Na-O2 Batteries.

Science.gov (United States)

Liu, Chenjuan; Carboni, Marco; Brant, William R; Pan, Ruijun; Hedman, Jonas; Zhu, Jiefang; Gustafsson, Torbjörn; Younesi, Reza

2018-04-25

Na-O 2 batteries are regarded as promising candidates for energy storage. They have higher energy efficiency, rate capability, and chemical reversibility than Li-O 2 batteries; in addition, sodium is cheaper and more abundant compared to lithium. However, inconsistent observations and instability of discharge products have inhibited the understanding of the working mechanism of this technology. In this work, we have investigated a number of factors that influence the stability of the discharge products. By means of in operando powder X-ray diffraction study, the influence of oxygen, sodium anode, salt, solvent, and carbon cathode were investigated. The Na metal anode and an ether-based solvent are the main factors that lead to the instability and decomposition of NaO 2 in the cell environment. This fundamental insight brings new information on the working mechanism of Na-O 2 batteries.

NaK handling and removal

International Nuclear Information System (INIS)

Desreumaux, J.; Rodriguez, G.; Guigon, A.; Verdelli, J.; Thomine, G.

1997-01-01

Sodium-potassium alloy is used in specific application in French Fast Breeder Reactors as: cold traps, NaK bubbler for argon purification, valves and also in experimental irradiation devices. lt has been preferred to sodium because it is liquid from + 7 deg. C for the most common peritectic alloy. After its use, NaK is considered as a hazardous waste (nuclear or not) due to its high reactivity with air and water. The most important risk remains in handling NaK systems which have not been operated for some time. The NaK will be covered with a crust of the superoxide K02 which is a strong oxidising agent. Thermodynamically, K02 will react with most organic material or metallic dust or swarfs and can also react with additional NaK to give sufficient heat to boil part of the NaK, resulting in a sudden increase in pressure and small explosions. We describe the formation given to experimenters in our Sodium School and the CEA's experience in treating specific devices for transportation, decanting of tanks, tank opening and NaK removal. (author)

Angiotensin II-induced hypertension increases plasma membrane Na pump activity by enhancing Na entry in rat thick ascending limbs.

Science.gov (United States)

Gonzalez-Vicente, Agustin; Garvin, Jeffrey L

2013-11-01

Thick ascending limbs (TAL) reabsorb 30% of the filtered NaCl load. Na enters the cells via apical Na-K-2Cl cotransporters and Na/H exchangers and exits via basolateral Na pumps. Chronic angiotensin II (ANG II) infusion increases net TAL Na transport and Na apical entry; however, little is known about its effects on the basolateral Na pump. We hypothesized that in rat TALs Na pump activity is enhanced by ANG II-infusion, a model of ANG II-induced hypertension. Rats were infused with 200 ng·kg(-1)·min(-1) ANG II or vehicle for 7 days, and TAL suspensions were obtained. We studied plasma membrane Na pump activity by measuring changes in 1) intracellular Na (Nai) induced by ouabain; and 2) ouabain-sensitive oxygen consumption (QO2). We found that the ouabain-sensitive rise in Nai in TALs from ANG II-infused rats was 12.8 ± 0.4 arbitrary fluorescent units (AFU)·mg(-1)·min(-1) compared with only 9.9 ± 1.1 AFU·mg(-1)·min(-1) in controls (P Na pump expression, the number of Na pumps in the plasma membrane, or the affinity for Na. When furosemide (1.1 mg·kg(-1)·day(-1)) was coinfused with ANG II, no increase in plasma membrane Na pump activity was observed. We concluded that in ANG II-induced hypertension Na pump activity is increased in the plasma membrane of TALs and that this increase is caused by the chronically enhanced Na entry occurring in this model.

Silicene for Na-ion battery applications

KAUST Repository

Zhu, Jiajie

2016-08-19

Na-ion batteries are promising candidates to replace Li-ion batteries in large scale applications because of the advantages in natural abundance and cost of Na. Silicene has potential as the anode in Li-ion batteries but so far has not received attention with respect to Na-ion batteries. In this context, freestanding silicene, a graphene-silicene-graphene heterostructure, and a graphene-silicene superlattice are investigated for possible application in Na-ion batteries, using first-principles calculations. The calculated Na capacities of 954mAh/g for freestanding silicene and 730mAh/g for the graphenesilicene superlattice (10% biaxial tensile strain) are highly competitive and potentials of >0.3 V against the Na/Na potential exceed the corresponding value of graphite. In addition, the diffusion barriers are predicted to be <0.3 eV.

Hydrolysis of Rice Straw Pretreated by Na2SO3 Over Fe-resin/NaCl

Directory of Open Access Journals (Sweden)

YANG Hui

2017-05-01

Full Text Available To increase the conversion of rice straw(RS and the yield of products, we employed three methods, which were ultrasonic wave, steam explosion and Na2SO3 pretreatment to pretreat RS(the treated RS noted as CS-RS, ZQ-RS and Na2SO3-RS, respectively and found that Na2SO3 treatment was the best pretreatment method based on XRD, SEM, elemental analysis and content of cellulose, hemicellulose and lignin. The conversion of Na2SO3-RS and the yield of total reducing sugar(TRS and levulinic acid(LA were 97.3%, 29.6% and 13.5%, respectively by 10% Fe-resin in 3.3% NaCl solution under 200 ℃.

Na+/H+ and Na+/NH4+ exchange activities of zebrafish NHE3b expressed in Xenopus oocytes

Science.gov (United States)

Ito, Yusuke; Kato, Akira; Hirata, Taku; Hirose, Shigehisa

2014-01-01

Zebrafish Na+/H+ exchanger 3b (zNHE3b) is highly expressed in the apical membrane of ionocytes where Na+ is absorbed from ion-poor fresh water against a concentration gradient. Much in vivo data indicated that zNHE3b is involved in Na+ absorption but not leakage. However, zNHE3b-mediated Na+ absorption has not been thermodynamically explained, and zNHE3b activity has not been measured. To address this issue, we overexpressed zNHE3b in Xenopus oocytes and characterized its activity by electrophysiology. Exposure of zNHE3b oocytes to Na+-free media resulted in significant decrease in intracellular pH (pHi) and intracellular Na+ activity (aNai). aNai increased significantly when the cytoplasm was acidified by media containing CO2-HCO3− or butyrate. Activity of zNHE3b was inhibited by amiloride or 5-ethylisopropyl amiloride (EIPA). Although the activity was accompanied by a large hyperpolarization of ∼50 mV, voltage-clamp experiments showed that Na+/H+ exchange activity of zNHE3b is electroneutral. Exposure of zNHE3b oocytes to medium containing NH3/NH4+ resulted in significant decreases in pHi and aNai and significant increase in intracellular NH4+ activity, indicating that zNHE3b mediates the Na+/NH4+ exchange. In low-Na+ (0.5 mM) media, zNHE3b oocytes maintained aNai of 1.3 mM, and Na+-influx was observed when pHi was decreased by media containing CO2-HCO3− or butyrate. These results provide thermodynamic evidence that zNHE3b mediates Na+ absorption from ion-poor fresh water by its Na+/H+ and Na+/NH4+ exchange activities. PMID:24401990

The relation between aging, aortic NaF avidity and coronary artery NaF avidity: A NaF PET CT study

DEFF Research Database (Denmark)

Blomberg, Björn; Thomassen, Anders; Hildebrandt, Malene

2013-01-01

volunteers without traditional cardiovascular risk factors were prospectively assessed by Sodium 18-Fluoride (Na-18F) PET CT imaging. Global aortic uptake of Na-18F was determined by calculating the average aortic blood pool subtracted maximum standardized uptake value (cSUV) [maximum SUVaorta - mean...

AlteraÃÃes MetabÃlicas e EnzimÃticas na RegeneraÃÃo HepÃtica em Ratos Alimentados com Dietas Suplementar de L-Glutamina e Alfa-Cetoglutarato

OpenAIRE

Artur Guimaraes Filho

2012-01-01

Estudos demonstraram que a hepatectomia parcial (HP) com remoÃÃo de dois terÃos do ÃrgÃo, em roedores, Ã seguida por crescimento do fÃgado remanescente. Em cÃlulas eucariontes a enzima malato desidrogenase, do ciclo de Krebs, pode ser encontrada no citoplasma (malato desidrogenase citoplÃsmica â (MDH1) e na matriz mitocondrial (malato desidrogenase mitocondrial - MDH2). A amÃnia, um composto quÃmico tÃxico para os animais, Ã produzida no curso do metabolismo. O fÃgado participa ativamente do ...

Rescue of Na+ and H+ binding in Na+,K+-ATPase M8 aspartate mutants by secondary mutation

DEFF Research Database (Denmark)

Holm, Rikke; Einholm, Anja P.; Andersen, Jens Peter

A mutation replacing the aspartate in transmembrane segment M8 in the a3-isoform of Na,K-ATPase with asparagine has been found in patients with rapid-onset dystonia parkinsonism or alternating hemiplegia of childhood. This aspartate may be a critical Na+ coordinating residue, but the crystal......-isoforms of Na,K-ATPase, and much smaller effects were seen for other mutations to the M8 aspartate, which were less disruptive of Na+ binding than mutations to other residues related to Na+ site III. The D928 (rat a1 numbering) mutations strongly diminished the cooperativity of Na+ binding. Moreover the p......H optimum of Na,K-ATPase activity was left-shifted, again with D928N being most disruptive. The reduced affinity for activating Na+ and for inhibitory protons, caused by D928N and D928A mutations, could be rescued by introduction of an additional mutation of a glutamate located far away from D928....

Salinity tolerance in barley (hordeum vulgare l.): effects of varying NaCl, K/sup +/ Na/sup +/ and NaHCO/sub 3/ levels on cultivars differing in tolerance

International Nuclear Information System (INIS)

Mahmood, K.

2011-01-01

Although barley (Hordeum vulgare L.) is regarded as salt tolerant among crop plants, its growth and plant development is severely affected by ionic and osmotic stresses in salt-affected soils. To elucidate the tolerance mechanism, growth and ion uptake of three barley cultivars, differing in salt tolerance, were examined under different levels of NaCl, K/sup +/ Na/sup +/ and NaHCO/sub 3/ in the root medium. The cultivars differed greatly in their responses to varying root medium conditions. Plant growth was more adversely affected by NaHCO/sub 3/ than NaCl. In general, biomass yields were comparable under control and 100 mM NaCl. However, growth of all three cultivars was significantly inhibited by NaHCO/sub 3/ even at low concentration (10 mM). Improved K/sup +/ supply in saline medium increased K/sup +/ uptake and growth of less tolerant cultivars. K/sup +/ uptake was more adversely affected by NaHCO/sub 3/ than NaCl salinity. Selective K/sup +/ uptake and lower Cl/sup -/ in shoots seemed to be associated with the growth responses. K application would help better growth of these cultivars on K-deficient saline-sodic soils and under irrigation with poor quality water having high Residual Sodium Carbonate (RSC) and/or Sodium Adsorption Ratio (SAR). (author)

Efeitos do alfa-tocoferol nas lesões de isquemia-reperfusão induzidas em pata direita de ratos

Directory of Open Access Journals (Sweden)

Bitu-Moreno José

2001-01-01

Full Text Available Recentes estudos demonstraram que os radicais livres de oxigênio parecem desempenhar papel importante nas lesões de isquemia-reperfusão. O alfa-tocoferol(AT age in vivo como um anti-oxidante. Nosso objetivo foi o de verificar se o AT atenuava as lesões de isquemia-reperfusão em pata direita de ratos. Os animais, randomizados, foram divididos nos seguintes grupos(G: G1-controle sem isquemia; G2 e G3 - quatro horas isquemia e duas reperfusão. Os animais do G2 foram tratados com salina e os do G3 com AT, 50mg/kg. Como parâmetros examinamos as alterações de volume e circunferência do pé direito ao longo do experimento, assim como a captação de 99mTc-pirofosfato e a microscopia eletrônica de transmissão do músculo soleus direito. Os aumentos de volume e circunferência do pé dos animais do G2 foram estatisticamente maiores quando comparados com o G1. As medidas do G3 não diferiram do G1. A captação de 99mTc-pirofosfato foi bem aumentada nos G2 e G3, quando comparados ao G1, porém sem diferenças entre os grupos isquêmicos. As lesões ao MET foram menos intensas em três animais do G3, quando comparados ao G2. Conclusão: O tratamento com AT diminuiu a formação do edema mas só protegeu parcialmente as células musculares contra as lesões de isquemia-reperfusão.

Glutathionylation-Dependence of Na+-K+-Pump Currents Can Mimic Reduced Subsarcolemmal Na+ Diffusion

OpenAIRE

Garcia, Alvaro; Liu, Chia-Chi; Cornelius, Flemming; Clarke, Ronald?J.; Rasmussen, Helge?H.

2016-01-01

The existence of a subsarcolemmal space with restricted diffusion for Na+ in cardiac myocytes has been inferred from a transient peak electrogenic Na+-K+ pump current beyond steady state on reexposure of myocytes to K+ after a period of exposure to K+-free extracellular solution. The transient peak current is attributed to enhanced electrogenic pumping of Na+ that accumulated in the diffusion-restricted space during pump inhibition in K+-free extracellular solution. However, there are no know...

Myocardial Na,K-ATPase: Clinical aspects

OpenAIRE

Kjeldsen, Keld

2003-01-01

The specific binding of digitalis glycosides to Na,K-ATPase is used as a tool for Na,K-ATPase quantification with high accuracy and precision. In myocardial biopsies from patients with heart failure, total Na,K-ATPase concentration is decreased by around 40%; a correlation exists between a decrease in heart function and a decrease in Na,K-ATPase concentration. During digitalization, around 30% of remaining pumps are occupied by digoxin. Myocardial Na,K-ATPase is also influenced by other drugs...

New digital ink for the obtention of innovating ceramics effects to obtain new possibilities and tendencies for the future; Este trabajo ha sido premiado con el Alfa de Oro en la Feria Internacional de Ceramica de Valencia CEVISAMA 2012

Energy Technology Data Exchange (ETDEWEB)

NONE

2012-07-01

This technical note contains the most significant aspects of the work New digital inks for the production of innovative ceramic effects which generate new possibilities and future trends in which the Group Torrecid won the Alfa de Oro 2012 given by the Spanish Society of Ceramics and Glass. To achieve the stated objectives to work in different fields of activity for each specific ink has been necessary, comprising, among other, aspects such as the development of special frits, selecting the most suitable solvents or optimizing the compositions to ensure that the inks have appropriate behaviour in the printing process and met all the requirements of stability and quality that the ceramic industry demand. (Author)

Estimadores de consistencia interna en las investigaciones en salud: el uso del coeficiente alfa

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Franciele Cascaes da Silva

Full Text Available La producción académica ha aumentado en el área de la salud, exigiendo cada vez más calidad en las publicaciones de gran impacto. Una de las formas de considerar la calidad es por medio de procedimientos que aumentan la consistencia del análisis de los datos, como la confiabilidad, que dependiendo del tipo de datos, puede ser evaluada por diferentes coeficientes, en especial el coeficiente alfa. Basados en lo anterior, la presente revisión reúne sistemáticamente artículos científicos producidos en los últimos cinco años, que de manera metodológica hicieron uso psicométrico del coeficiente α como un estimador de consistencia interna y de confiabilidad en procesos de construcción, adaptación y validación de instrumentos. La identificación de los estudios fue realizada sistemáticamente en las bases de datos Biomed Central Journals, Google Scholar, Web of Science, Wiley Online Library, Medline via Pubmed, Scielo, Scopus, Journals@Ovid, BMJ y Springer por medio del Portal de Periódicos CAPES, utilizando criterios de inclusión y exclusión. El análisis de los datos fue realizado por medio de triangulación, análisis de contenido y análisis descriptivo. Se obtuvo que la mayoría de los estudios fueron realizados principalmente en Irán (f=3, España (f=2 y Brasil (f=2, estos estudios tuvieron como objetivo testar las propiedades psicométricas de instrumentos, siendo que ocho estudios utilizaron el coeficiente α para evaluar la confiabilidad y nueve para evaluar la consistencia interna. Todos los estudios fueron clasificados como investigaciones metodológicas al analizar sus objetivos. Adicionalmente, cuatro estudios también fueron clasificados como correlacionales y uno como descriptivo-correlacional. Se puede concluir que a pesar de que el coeficiente α es ampliamente utilizado como uno de los principales parámetros para la evaluación de consistencia interna de instrumentos de medición del tipo cuestionario en el �

Down-Regulation of the Na+-Coupled Phosphate Transporter NaPi-IIa by AMP-Activated Protein Kinase

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Miribane Dërmaku-Sopjani

2013-11-01

Full Text Available Background/Aims: The Na+-coupled phosphate transporter NaPi-IIa is the main carrier accomplishing renal tubular phosphate reabsorption. It is driven by the electrochemical Na+ gradient across the apical cell membrane, which is maintained by Na+ extrusion across the basolateral cell membrane through the Na+/K+ ATPase. The operation of NaPi-IIa thus requires energy in order to avoid cellular Na+ accumulation and K+ loss with eventual decrease of cell membrane potential, Cl- entry and cell swelling. Upon energy depletion, early inhibition of Na+-coupled transport processes may delay cell swelling and thus foster cell survival. Energy depletion is sensed by the AMP-activated protein kinase (AMPK, a serine/threonine kinase stimulating several cellular mechanisms increasing energy production and limiting energy utilization. The present study explored whether AMPK influences the activity of NAPi-IIa. Methods: cRNA encoding NAPi-IIa was injected into Xenopus oocytes with or without additional expression of wild-type AMPK (AMPKα1-HA+AMPKβ1-Flag+AMPKγ1-HA, of inactive AMPKαK45R (AMPKα1K45R+AMPKβ1-Flag+AMPKγ1-HA or of constitutively active AMPKγR70Q (AMPKα1-HA+AMPKβ1-Flag+AMPKγ1R70Q. NaPi-IIa activity was estimated from phosphate-induced current in dual electrode voltage clamp experiments. Results: In NaPi-IIa-expressing, but not in water-injected Xenopus oocytes, the addition of phosphate (1 mM to the extracellular bath solution generated a current (Ip, which was significantly decreased by coexpression of wild-type AMPK and of AMPKγR70Q but not of AMPKαK45R. The phosphate-induced current in NaPi-IIa- and AMPK-expressing Xenopus ooocytes was significantly increased by AMPK inhibitor Compound C (20 µM. Kinetic analysis revealed that AMPK significantly decreased the maximal transport rate. Conclusion: The AMP-activated protein kinase AMPK is a powerful regulator of NaPi-IIa and thus of renal tubular phosphate transport.

Thermodynamic study of NaFe complex oxides. High temperature properties of Na sub 5 FeO sub 4 and Na sub 3 FeO sub 3

CERN Document Server

Furukawa, T

2002-01-01

In order to contribute the investigation into corrosion mechanism of the structural materials by leakage sodium, thermodynamic study of Na-Fe complex oxides formed by the reactions was carried out. Na sub 5 FeO sub 4 and Na sub 3 FeO sub 3 were used as the sample. Its high temperature properties (i.e. melting, solidification and transformation) were observed by Differential Scanning Calorimetry, DSC. Moreover, the original test named 'melting point confirmation test' was performed for the observation of traces of melting and solidification after the tests. Following contents have been obtained by this study. (1) Na sub 5 FeO sub 4 was stably as the solid without phase transformation and melting until 800degC. However, the compound was showing a tendency to change into Na sub 4 FeO sub 3 with temperature increasing under the low oxygen potential. (2) The stability of Na sub 3 FeO sub 3 is the same as that of Na sub 5 FeO sub 4 until 700degC. Over the temperature, the compound was changed differential compound ...

Metallic Na formation in NaCl crystals with irradiation of electron or vacuum ultraviolet photon

Energy Technology Data Exchange (ETDEWEB)

Owaki, Shigehiro [Osaka Prefecture Univ., Sakai, Osaka (Japan). Coll. of Integrated Arts and Sciences; Koyama, Shigeko; Takahashi, Masao; Kamada, Masao; Suzuki, Ryouichi

1997-03-01

Metallic Na was formed in NaCl single crystals with irradiation of a variety of radiation sources and analyzed the physical states with several methods. In the case of irradiation of 21 MeV electron pulses to the crystal blocks, the optical absorption and lifetime measurement of positron annihilation indicated appearance of Na clusters inside. Radiation effects of electron beam of 30 keV to the crystals in vacuum showed the appearance of not only metallic Na but atomic one during irradiation with Auger electron spectroscopy. Intense photon fluxes in vacuum ultraviolet region of synchrotron radiation were used as another source and an analyzing method of ultraviolet photoelectron spectroscopy. The results showed the metallic Na layered so thick that bulk plasmon can exist. (author)

Estudo comparativo do uso de Medetomidina e Xilazina em gatos e sua reversão com Atipamezole

OpenAIRE

Cardoso, Cléber Salesse

2009-01-01

A medetomidina um sedativo e analgésico do grupo dos agonistas alfa2 adrenérgicos aprovada para cães e gatos, descrita atualmente como mais potente do que a xilazina, antiga agonista alfa2 adrenérgica de uso veterinário. A principal diferença entre esses dois agonistas é a seletividade aos receptores alfa2-adrenérgicos, sendo a medetomidina considerada mais seletiva, conferindo, portanto, maior potência sedativa e analgésica. A reversão dos efeitos dos agonistas alfa2-adrenérgicos é realizada...

Avaliação do desempenho organizacional da empresa familiar Indústrias Alfa S.A.* com base no modelo de gestão estratégica balanced scorecard Organizational performance of a family business evaluated by the strategic performance balanced scorecard

Directory of Open Access Journals (Sweden)

Christine Schneider

2006-03-01

Full Text Available O balanced scorecard é um moderno modelo estratégico de desempenho organizacional que visa traduzir a missão, visão e estratégia em objetivos e medidas, organizados sob a ótica financeira, dos clientes, dos processos internos, do aprendizado e do crescimento. O modelo disponibiliza estrutura e linguagem para comunicar a estratégia da empresa e o alcance das metas no longo prazo. A Indústrias Alfa S.A. é uma empresa familiar de segunda geração, que tem por visão estratégica "Ser a escolha preferencial de soluções hidráulicas da América Latina nos setores residencial, industrial, agrícola e predial", orientada pelo macroobjetivo de oferecer soluções e serviços em motobombas hidráulicas de modo a satisfazer as necessidades dos clientes. Partindo desses princípios, o artigo avalia o desempenho organizacional da empresa familiar Indústrias Alfa S.A. no primeiro quadrimestre do ano de 2005, com base no modelo de controle estratégico balanced scorecard.The balanced scorecard is a modern strategic model of organizational performance that seeks to translate the company mission, vision and strategy into objectives and measures organized to focus on - finance, customers, internal procedures, learning and growth. This model provides structure and language to communicate business strategy and directs efforts, abilities and specific knowledge to achieve long term objectives. The strategic vision of this second generation family business is "to be the answer for hydraulic pump needs in the Latin American residential, industrial, agricultural and building management markets" oriented by an overall objective of offering solutions and services to satisfy customer needs. Based upon these considerations, performance of this family business was evaluated during the first four months of 2005 using the strategic performance balanced scorecard.

Scintillation efficiency measurement of Na recoils in NaI(Tl) below the DAMA/LIBRA energy threshold

Energy Technology Data Exchange (ETDEWEB)

Xu, Jingke; Shields, Emily; Calaprice, Frank; Westerdale, Shawn; Froborg, Francis; Suerfu, Burkhant; Alexander, Thomas; Aprahamian, Ani; Back, Henning O.; Casarella, Clark; Fang, Xiao; Gupta, Yogesh K.; Ianni, Aldo; Lamere, Edward; Lippincott, W. Hugh; Liu, Qian; Lyons, Stephanie; Siegl, Kevin; Smith, Mallory; Tan, Wanpeng; Kolk, Bryant Vande

2015-07-01

The dark matter interpretation of the DAMA modulation signal depends on the NaI(Tl) scintillation efficiency of nuclear recoils. Previous measurements for Na recoils have large discrepancies, especially in the DAMA/LIBRA modulation energy region. We report a quenching effect measurement of Na recoils in NaI(Tl) from 3 to 52 keVnr, covering the whole DAMA/LIBRA energy region for dark matter-Na scattering interpretations. By using a low-energy, pulsed neutron beam, a double time-of-flight technique, and pulse-shape discrimination methods, we obtained the most accurate measurement of this kind for NaI(Tl) to date. The results differ significantly from the DAMA reported values at low energies but fall between the other previous measurements. We present the implications of the new quenching results for the dark matter interpretation of the DAMA modulation signal.

Acessibilidade e paralelismo na interpretação do pronome sujeito e o contraste pro/pronome em português Accessibility, parallel function and the pro/pronoun contrast in Portuguese

Directory of Open Access Journals (Sweden)

Letícia Maria Sicuro CORRÊA

1998-01-01

Full Text Available O contraste pro/pronome em orações coordenadas em português é aqui explorado de modo a distinguirem-se os procedimentos através dos quais formas pronominais sujeito são interpretadas em diferentes contextos sintáticos e discursivos — através de estratégias de seleção de um antecedente lingüístico ou da recuperação "automática" de uma representação mantida particularmente ativada na memória de trabalho. Dois experimentos são relatados. O primeiro testa a hipótese de que o vínculo sintático entre a oração que contém a forma pronominal e a que contém seus possíveis antecedentes define condições de processamento que favorecem o uso de um ou de outro procedimento de interpretação. O segundo testa a hipótese de que o grau de ativação de uma dada representação na memória de trabalho (definido em relação a um sistema que opera em três níveis afeta o modo como o sujeito pronominal de orações independentes é interpretado no discurso. Verifica-se que o contraste pro/ pronome pode ser re-estabelecido fora do âmbito de sentenças complexas uma vez que haja alteração local do foco da referência. A natureza deste contraste é discutida levando-se em conta as condições de processamento nas quais este se manifesta.The contrastive interpretation of pro and pronoun in co-ordinate sentences by Portuguese speakers is explored here, as a means of distinguishing the procedures whereby pronominal forms are interpreted in different syntactic/discourse contexts - by means of a search-identification strategy (such as the parallel function strategy or by means of the "automatic" recovery of a representation which is maintained particularly activated in working memory. Two experiments are reported. The first one tests the hypothesis that the kind of syntactic relationship that holds between the clause containing the pronominal form and the one containing possible antecedents defines processing conditions that favour one

Synthesis of Ag or Pt Nanoparticles by Hydrolysis of Either Ag2Na or PtNa

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Huabin Wang

2008-01-01

Full Text Available Ag and Pt nanoparticles have successfully been synthesized by hydrolysis of either Ag2Na or PtNa at room temperature. The oxidation of sodium in the Pt-Na pellets was much faster than that in the Ag-Na pellets since Pt is a catalyst for H2O formation reaction from hydrogen and oxygen at room temperature. The hydrolysis byproduct, NaOH, has a high solubility and easily is removed. This method offers a simple method of preparing transition metal nanoparticles. The Ag and Pt nanoparticles prepared by this method were crystalline in nature, and spherical in shape with a mean size of around 10 nm.

Dynamic polarizabilities and Van der Waals coefficients for alkali atoms Li, Na and alkali dimer molecules Li2, Na2 and NaLi

Science.gov (United States)

Mérawa, M.; Dargelos, A.

1998-07-01

The present paper gives an account of investigations of the polarizability of the alkali atoms Li, Na, diatomics homonuclear and heteronuclear Li2, Na2 and NaLi at SCF (Self Consistent Field) level of approximation and at correlated level, using a time Time-Dependent Gauge Invariant method (TDGI). Our static polarizability values agree with the best experimental and theoretical determinations. The Van der Waals C6 coefficients for the atom-atom, atom-dimer and dimer-dimer interactions have been evaluated. Les polarisabilités des atomes alcalins Li, Na, et des molécules diatomiques homonucléaires et hétéronucléaire Li2, Na2 et NaLi, ont été calculées au niveau SCF (Self Consistent Field) et au niveau corrélé à partir d'une méthode invariante de jauge dépendante du temps(TDGI). Nos valeurs des polarisabilités statiques sont en accord avec les meilleurs déterminations expérimentales et théoriques. Les coefficients C6 de Van de Waals pour les interactions atome-atome, atome-dimère et dimère-dimère ont également été évalués.

Structure-function relationships of Na+, K+, ATP, or Mg2+ binding and energy transduction in Na,K-ATPase

DEFF Research Database (Denmark)

Jorgensen, Peter L.; Pedersen, Per Amstrup

2000-01-01

Na,K-ATPase; Mutagenesis; Na+ binding; K+ binding; Tl+ binding; Mg2+ binding; ATP binding; Cation binding site; Energy transduction......Na,K-ATPase; Mutagenesis; Na+ binding; K+ binding; Tl+ binding; Mg2+ binding; ATP binding; Cation binding site; Energy transduction...

At NA2

CERN Multimedia

1977-01-01

One of the NA2 calorimeter sections is moved in. The NA2 calorimeter was divided in two halves, to the left and the right of the beam, each half consisting of sheets of passive high Z material interleaved with blades of plastic scintillators. The photo shows on the right, the upstream 'electron' module with eleven lead plates for a total radiation length of 20.

The use of recombinant human LH (lutropin alfa) in the late stimulation phase of assisted reproduction cycles: a double-blind, randomized, prospective study.

Science.gov (United States)

Tarlatzis, B; Tavmergen, E; Szamatowicz, M; Barash, A; Amit, A; Levitas, E; Shoham, Z

2006-01-01

The effect of recombinant human LH (r-hLH; lutropin alfa) in women undergoing controlled ovarian stimulation with recombinant human FSH (r-hFSH) prior to IVF was investigated. After down-regulation with the GnRH agonist, buserelin, 114 normo-ovulatory women (aged 18-37 years) received r-hFSH alone until the lead follicle reached a diameter of 14 mm. Patients were then randomized in a double-blind fashion to receive r-hFSH in addition to r-hLH, 75 IU s.c., or placebo daily for a maximum of 10 days prior to oocyte retrieval and IVF. The primary end-point was the number of metaphase II oocytes. There were no significant differences between treatment groups for the primary end-point. Serum estradiol concentrations on the day of HCG administration were significantly higher in the group receiving r-hLH plus r-hFSH than in the group receiving r-hFSH alone (P = 0.0001), but there were no significant differences between the groups in dose and duration of r-hFSH treatment required, oocyte maturation, fertilization rate, pregnancy rate and live birth rate. In this patient population, the addition of r-hLH during the late follicular phase of a long GnRH agonist and r-hFSH stimulation cycle provides no further benefit in terms of oocyte maturation or other end-points.

Postura da mão e imagética motora: um estudo sobre reconhecimento de partes do corpo Hand posture and motor imagery: a body-part recognition study

Directory of Open Access Journals (Sweden)

AP Lameira

2008-10-01

Full Text Available OBJETIVOS: Assim como a imagética motora, o reconhecimento de partes do corpo aciona representações somatosensoriais específicas. Essas representações são ativadas implicitamente para comparar o corpo com o estímulo. No presente estudo, investigou-se a influência da informação proprioceptiva da postura no reconhecimento de partes do corpo (mãos e propõe-se a utilização dessa tarefa na reabilitação de pacientes neurológicos. MATERIAIS E MÉTODOS: Dez voluntários destros participaram do experimento. A tarefa era reconhecer a lateralidade de figuras da mão apresentada, em várias perspectivas e em vários ângulos de orientação. Para a figura da mão direita, o voluntário pressionava a tecla direita e para a figura da mão esquerda, a tecla esquerda. Os voluntários realizavam duas sessões: uma com as mãos na postura prona e outra com as mãos na postura supina. RESULTADOS: Os tempos de reação manual (TRM eram maiores para as vistas e orientações, nas quais é difícil realizar o movimento real, mostrando que durante a tarefa, existe um acionamento de representações motoras para comparar o corpo com o estímulo. Além disso, existe uma influência da postura do sujeito em vistas e ângulos específicos. CONCLUSÕES: Estes resultados mostram que representações motoras são ativadas para comparar o corpo com o estímulo e que a postura da mão influencia esta ressonância entre estímulo e parte do corpo.OBJECTIVE: Recognition of body parts activates specific somatosensory representations in a way that is similar to motor imagery. These representations are implicitly activated to compare the body with the stimulus. In the present study, we investigate the influence of proprioceptive information relating to body posture on the recognition of body parts (hands. It proposes that this task could be used for rehabilitation of neurological patients. METHODS: Ten right-handed volunteers participated in this experiment. The

Na+-K+ pump in chronic renal failure

International Nuclear Information System (INIS)

Deepak, K.; Kahn, T.

1987-01-01

This review summarizes the evidence for the defect in Na + -K + pump in chronic renal failure, considers the role of various factors in causing this defect, and discusses the clinical implications thereof. Intracellular Na is elevated in erythrocytes, leukocytes, and muscle cells from some patients with chronic renal failure (CRF). Recent evidence suggest that this elevation of cell Na may be, in large part, a consequence of decreased number of Na + -K + pump units per cell. Maintenance dialysis over a period of weeks ameliorates the defect in intracellular Na + , and this improvement is contemporaneous with an increase in the number of Na + -K + pump sites per cell. In erythrocytes with normal cell Na + , acute hemodialysis increases the rate of 22 Na + and 42 K + transport. Many factors such as the presence of retained toxic metabolite or circulating inhibitor in the uremic plasma, or biochemical changes produced by acute hemodialysis, may explain this finding. In cells with high cell Na + , the pump-mediated 42 K + transport is normalized at the expense of a raised cell Na + . The decreased muscle membrane potential in uremic subjects has been attributed to a decreased activity of Na + -K + pump. The authors discuss the role of hormonal abnormalities and circulating inhibitors, which may cause an acute inhibition of the pump and of other factors such as K + depletion, which may cause more chronic alterations. The implications of alteration of Na + and K + pump transport and raised cell Na + on other non-pump-mediated transport pathways are discussed. Raised cell Na + may be a marker for the adequacy of maintenance dialysis in patients with end-stage renal failure

Pozitivni in negativni vplivi risank na otroke

OpenAIRE

Povše, Nika

2017-01-01

V tej diplomski nalogi smo raziskovali pozitivne in negativne vplive risank na otroke. Začeli smo z zgodovino televizije, tako svetovno zgodovino kot zgodovino televizije na Slovenskem. Na isti način smo pregledali tudi zgodovino risank. Raziskovali smo otrokov razvoj, predvsem tiste vidike, ki so izpostavljeni vplivom risank, kot so morala, jezik, domišljija in učenje. Sledilo je raziskovanje negativnih in pozitivnih vplivov, ki jih imajo risanke na otroke, kar je tudi naš glavni problem te ...

Vpliv konfucija na sodobno izobraževanje na Kitajskem

Directory of Open Access Journals (Sweden)

Katja Skitek

2009-12-01

Full Text Available Izobraževanje na Kitajskem je bilo vedno trdno povezano s Konfucijem in konfucianizmom, kar pa ni čudno, saj je bil Konfucij prvi, ki je poudarjal moč z nanja in izobraževanja. LR Kitajska je v zadnjih petdesetih letih počasi in postopoma začela uveljavljati določene spremembe v vzgojno-izobraževalni sistem. Kakšne so te spremembe in koliko nanje še vedno vpliva konfucianizem, bo osrednja tema pričujočega članka. Konfucianizem danes na Kitajskem ni preveč popularen, izobraženci pa so se razporedili v dve skupini: eni trdijo, da je njegov vpliv še vedno velik, drugi pa zatrjujejo, da nima več nobenega vpliva na sodobno življenje. Katera stran ima prav? Konfucij je v prvi vrsti poudarjal pomembnost moralnih vrednot vsakega človeka in prav moralne vrednote, za katere se je zavzemal, so še danes trdno zasidrane v kitajski kulturi. Ali je torej sploh mogoče popolnoma izriniti Konfucija iz sodobnega izobraževanja?

Low-spin states of 23Na

International Nuclear Information System (INIS)

Bakkum, E.L.

1987-01-01

A study of 23 Na via the 22 Ne(p,γ) 23 Na and 23 Na(γ,γ) 23 Na reactions is presented. Only a limited number of resonances has been studied, selected on the basis of strong excitation of the lowest levels of which the spin was unknown. As a result the spins are now known of all levels of 23 Na with excitation energies up to 7 MeV, except for a few high-spin states which are too weakly excited in the decay of the known 22 Ne(p,γ) resonances. The mean lifetimes of the 23 Na levels at 4.43 and 7.89 MeV were found to be 350±70 and 220±17 attoseconds (1 attosecond = 10 -18 seconds) respectively. 97 refs.; 22 figs.; 12 tabs

Response of saliva Na/K ratio to changing Na supply of lactating cows under tropical conditions

NARCIS (Netherlands)

Thiangtum, Wandee; Schonewille, J Thomas; Verstegen, Martin Wa; Arsawakulsudhi, Supot; Rukkwamsuk, Theera; Hendriks, Wouter H

BACKGROUND: Factorial determination of the sodium (Na) requirement of heat-stressed lactating cows is hindered by accurate estimates of the Na losses through sweat. Direct studies, therefore, may be needed requiring information on the time course of healthy animals to become Na depleted and the

Response of saliva Na/K ratio to changing Na supply of lactating cows under tropical conditions

NARCIS (Netherlands)

Thiangtum, Wandee; Schonewille, Thomas J.; Verstegen, Martin W.A.; Arsawakulsudhi, Supot; Rukkwamsuk, Theera; Hendriks, Wouter H.

2017-01-01

BACKGROUND: Factorial determination of the sodium (Na) requirement of heat-stressed lactating cows is hindered by accurate estimates of the Na losses through sweat. Direct studies, therefore, may be needed requiring information on the time course of healthy animals to become Na depleted and the

Clues to NaCN formation

Science.gov (United States)

Quintana-Lacaci, G.; Cernicharo, J.; Velilla Prieto, L.; Agúndez, M.; Castro-Carrizo, A.; Fonfría, J. P.; Massalkhi, S.; Pardo, J. R.

2017-11-01

Context. ALMA is providing us essential information on where certain molecules form. Observing where these molecules emission arises from, the physical conditions of the gas, and how this relates with the presence of other species allows us to understand the formation of many species, and to significantly improve our knowledge of the chemistry that occurs in the space. Aims: We studied the molecular distribution of NaCN around IRC +10216, a molecule detected previously, but whose origin is not clear. High angular resolution maps allow us to model the abundance distribution of this molecule and check suggested formation paths. Methods: We modeled the emission of NaCN assuming local thermal equilibrium (LTE) conditions. These profiles were fitted to azimuthal averaged intensity profiles to obtain an abundance distribution of NaCN. Results: We found that the presence of NaCN seems compatible with the presence of CN, probably as a result of the photodissociation of HCN, in the inner layers of the ejecta of IRC +10216. However, similar as for CH3CN, current photochemical models fail to reproduce this CN reservoir. We also found that the abundance peak of NaCN appears at a radius of 3 × 1015 cm, approximately where the abundance of NaCl, suggested to be the parent species, starts to decay. However, the abundance ratio shows that the NaCl abundance is lower than that obtained for NaCN. We expect that the LTE assumption might result in NaCN abundances higher than the real ones. Updated photochemical models, collisional rates, and reaction rates are essential to determine the possible paths of the NaCN formation. Based on observations carried out with ALMA and the IRAM 30 m Telescope. ALMA is a partnership of ESO (representing its member states), NSF (USA) and NINS (Japan), together with NRC (Canada) and NSC and ASIAA (Taiwan), in cooperation with the Republic of Chile. The Joint ALMA Observatory is operated by ESO, AUI/NRAO and NAOJ. IRAM is supported by INSU/CNRS (France

Polimorfismos genéticos e desempenho físico em jogadores de futebol das categorias de base do São Paulo Futebol Clube

OpenAIRE

Thiago José Dionísio

2014-01-01

Há relatos na literatura de que os polimorfismos genéticos podem determinar importantes modulações nos fenótipos dos atletas, como por exemplo, estatura, adaptações cardiovasculares, utilização dos substratos energéticos bem como balanço eletrolítico e hormonal. É possível que indivíduos que expressem o gene alfa actinina 3 (ACTN3; genótipos RR para homozigotos ancestrais ou RX para heterozigotos) possam apresentar vantagens em movimentos que exijam força e rápida contração muscular quando co...

Memória em dislexia do desenvolvimento e surdez congênita: comparando arquiteturas cognitivas

Directory of Open Access Journals (Sweden)

Fernando C. Capovilla

2011-11-01

Full Text Available O modelo de memória verbal de Baddeley é usado para comparar o processamento de informação em dislexia do desenvolvimento e surdez congênita profunda. Em curvas de posição serial, o efeito de primazia revela a integridade do circuito fonológico, que permite reter fonologicamente a informação e consolidá-la na memória; o de recência, a integridade do circuito fonético, que permite reter articulatoriamente a informação sob audição e leitura orofacial. O Circuito Fonológico é composto de duas alças: uma, conectando Entrada a Saída, permite repetir pseudopalavras ouvidas; outra, conectando Saída a Entrada, permite ouvir-se pensando, refrescar a informação fonológica e memorizá-la. A alça conectando Entrada a Saída está comprometida na afasia de condução gerando inabilidade de repetir pseudopalavras ouvidas. A alça conectando Saída a Entrada está comprometida na dislexia de desenvolvimento, dificultando ouvir internamente o fluxo da fala durante leitura. O Circuito Fonético opera sempre que a fala é ouvida, articulada oralmente, ou lida orofacialmente. Nele, a Entrada é ativada na audição ou leitura orofacial; a Saída, na articulação subvocal. A alça conectando Entrada a Saída permite repetir pseudopalavras lidas orofacialmente. A alça conectando Saída a Entrada produz efeito de recência, permitindo manter traços de memória recente e elos da fala interna. Diferente da surdez congênita em que estão preservadas, na dislexia do desenvolvimento, as alças fonológica e fonética conectando Saída a Entrada estão comprometidas, dificultando o monitoramento do próprio ouvido interno e da própria voz interna durante a leitura e a escrita.Palavras-chave: Memória. Dislexia. Surdez. Fonologia. Fonética.

The effect of Na+ and K+ on the thermal denaturation of Na+ and + K+-dependent ATPase.

Science.gov (United States)

Fischer, T H

1983-01-01

To increase our understanding of the physical nature of the Na+ and K+ forms of the Na+ + K+-dependent ATPase, thermal-denaturation studies were conducted in different types of ionic media. Thermal-denaturation measurements were performed by measuring the regeneration of ATPase activity after slow pulse exposure to elevated temperatures. Two types of experiments were performed. First, the dependence of the thermal-denaturation rate on Na+ and K+ concentrations was examined. It was found that both cations stabilized the pump protein. Also, K+ was a more effective stabilizer of the native state than was Na+. Secondly, a set of thermodynamic parameters was obtained by measuring the temperature-dependence of the thermal-denaturation rate under three ionic conditions: 60 mM-K+, 150 mM-Na+ and no Na+ or K+. It was found that ion-mediated stabilization of the pump protein was accompanied by substantial increases in activation enthalpy and entropy, the net effect being a less-pronounced increase in activation free energy. PMID:6309139

Financiranje projekata na pomorskom dobru

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Zoran Tasić

2018-02-01

Full Text Available Koncesije otvaraju vrlo osjetljiva politička, ekonomska i pravna pitanja u mnogim državama svijeta. Samo definiranje pojma koncesije često je komplicirana i ne sasvim jasna zadaća. OECD je prihvatila definiciju koncesije kao: ’’davanje prava privatnom trgovačkom društvu da može gospodarski upravljati određenom infrastrukturnim uslugama i za to zarađivati naknadu. Koncesionar dobija u posjed određene objekte (ali vlasništvo na tim objektima ostaje u rukama države te ih koristi u svrhu obavljanja određene djelatnosti ili usluge sukladno odredbama ugovora’’. Hrvatsko pravo definira koncesije načelno kao pravo stečeno temeljem ugovora o koncesiji, a sukladno uvjetima sadržanim u odluci o davanju koncesije (koju je donijelo odgovarajuće upravno tijelo, a u odnosu na pomorsko dobro, specifično, kao pravo državne vlasti da može dio pomorskog dobra, koje je inače dobro izvan pravnog prometa (res extra commercium, isključiti iz opće uporabe bilo dijelom ili u cijelosti, i dati ga fizičkoj ili pravnoj osobi na gospodarsko korištenje sukladno određenim prostornim planovima. Građevine i drugi objekti izgrađeni na pomorskom dobru čine njegov sastavni dio. Zakonom je jasno određeno da nikakva stvarna prava nisu dopuštena na pomorskom dobru, uključivo i na objektima izgrađenima na njemu. Projekti na pomorskom dobru često uključuju velike infrastrukturne projekte, razvoje luka i marina, koji iziskuju značajna financijska sredstva. Banke koje financiraju takve projekte zahtijevaju organiziran i transparentan zakonski okvir koncesija, slobodu ugovaranja, pravnu zaštitu svih strana uključenih u projekt (uključivo i mogućnost međunarodne arbitraže. (...

Controlled phase stability of highly Na-active triclinic structure in nanoscale high-voltage Na2-2xCo1+xP2O7 cathode for Na-ion batteries

Science.gov (United States)

Song, Hee Jo; Kim, Jae-Chan; Dar, Mushtaq Ahmad; Kim, Dong-Wan

2018-02-01

With the increasing demand for high energy density in energy-storage systems, a high-voltage cathode is essential in rechargeable Li-ion and Na-ion batteries. The operating voltage of a triclinic-polymorph Na2CoP2O7, also known as the rose form, is above 4.0 V (vs. Na/Na+), which is relatively high compared to that of other cathode materials. Thus, it can be employed as a potential high-voltage cathode material in Na-ion batteries. However, it is difficult to synthesize a pure rose phase because of its low phase stability, thus limiting its use in high-voltage applications. Herein, compositional-engineered, rose-phase Na2-2xCo1+xP2O7/C (x = 0, 0.1 and 0.2) nanopowder are prepared using a wet-chemical method. The Na2-2xCo1+xP2O7/C cathode shows high electrochemical reactivity with Na ions at 4.0 V, delivering high capacity and high energy density.

Total Counting and Spectroscopy in the Assessment of Alpha Radioactivity in Human Tissues; Determination de la Radioactivite Alpha des Tissus Humains par Dosage de l'Activite Globale et Spectroscopie; Obshchee izmerenie i spektroskopiya pri opredelenii al'fa-radioaktivnosti tkanej cheloveka; Evaluacion de la Actividad Alfa en los Tejidos Humanos por Recuento Global y Espectroscopia

Energy Technology Data Exchange (ETDEWEB)

Mayneord, W. V.; Hill, C. R. [Physics Department, Institute of Cancer Research, Royal Cancer Hospital, Clifton Avenue, Belmont, Surrey (United Kingdom)

1964-10-15

atmosfera y se citan ejemplos de los resultados de las mediciones de aire ligeramente contaminado por esa sustancia. Se describen asimismo procedimientos de medicion del {sup 239}Pu en tejidos humanos normales y se exponen algunos resultados. Se estudia la contribucion aportada por el {sup 210}Po a la dosis de irradiacion natural proveniente del medio ambiente en el esqueleto y en los organos reproductores del hombre. Se demuestra que esa aportacion puede constituir una fraccion significativa de la dosis total recibida. (author) [Russian] Daetsja kratkij obzor programmy po izmereniju al'fa- radioaktivnosti. Vypolnenie programmy, kotoroe pervonachal'no osnovyvalos' na ispol'zovanii prostyh i chuvstvitel'nyh metodov podscheta s pomoshh'ju tonkih cink-kadmij-sul'fidnyh jekranov teper' vkljuchaet ispol'zovanie metoda al'fa-spektroskopii, razrabotannogo dlja bol'shogo kolichestva istochnikov s nizkoj specificheskoj aktivnost'ju. S pomoshh'ju jetih metodov mozhno izmerjat' urovni specificheskoj aktivnosti vplot' do 10{sup -13} kjuri/g i v nekotoryh sluchajah poluchat' razreshenie jenergii porjadka 40 kjev narjadu s nezavisimoj identifikaciej, osnovannoj na izmerenii perioda poluraspada. Daetsja opisanie dvuh al'fa-spektrometrov, ispol'zovannyh pri izmerenii bol'shogo kolichestva obrazcov, vzjatyh u ljudej, i materialov okruzhajushhej sredy. Osoboe vnimanie udeljalos' izucheniju Po{sup 210} , kotoryj, kak izvestno, prisutstvuet v rjade pishhevyh produktov i tkanjah chelovecheskogo organizma. Opisany metody issledovanija Pu{sup 239} v atmosfere i privedeny primery rezul'tatov izmerenija zagrjaznenija vozduha malymi kolichestvami jetogo materiala. Opisany metody izmerenija Pu{sup 239} v normal'nyh tkanjah u cheloveka i privedeny nekotorye rezul'taty. Obsuzhdaetsja rol' Ro{sup 210} v povyshenii urovnja estestvennogo fonovogo obluchenija kostej i reproduktivnyh organov cheloveka. Pokazano, chto jeta dolja mozhet sostavljat' znachitel'nuju chast' obshej poluchennoj dozy. (author)

Optimization of NaOH Molarity, LUSI Mud/Alkaline Activator, and Na2SiO3/NaOH Ratio to Produce Lightweight Aggregate-Based Geopolymer

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Rafiza Abdul Razak

2015-05-01

Full Text Available This paper presents the mechanical function and characterization of an artificial lightweight geopolymer aggregate (ALGA using LUSI (Sidoarjo mud and alkaline activator as source materials. LUSI stands for LU-Lumpur and SI-Sidoarjo, meaning mud from Sidoarjo which erupted near the Banjarpanji-1 exploration well in Sidoarjo, East Java, Indonesia on 27 May 2006. The effect of NaOH molarity, LUSI mud/Alkaline activator (LM/AA ratio, and Na2SiO3/NaOH ratio to the ALGA are investigated at a sintering temperature of 950 °C. The results show that the optimum NaOH molarity found in this study is 12 M due to the highest strength (lowest AIV value of 15.79% with lower water absorption and specific gravity. The optimum LUSI mud/Alkaline activator (LM/AA ratio of 1.7 and the Na2SiO3/NaOH ratio of 0.4 gives the highest strength with AIV value of 15.42% with specific gravity of 1.10 g/cm3 and water absorption of 4.7%. The major synthesized crystalline phases were identified as sodalite, quartz and albite. Scanning Electron Microscope (SEM image showed more complete geopolymer matrix which contributes to highest strength of ALGA produced.

Optimization of NaOH Molarity, LUSI Mud/Alkaline Activator, and Na2SiO3/NaOH Ratio to Produce Lightweight Aggregate-Based Geopolymer

Science.gov (United States)

Abdul Razak, Rafiza; Abdullah, Mohd Mustafa Al Bakri; Hussin, Kamarudin; Ismail, Khairul Nizar; Hardjito, Djwantoro; Yahya, Zarina

2015-01-01

This paper presents the mechanical function and characterization of an artificial lightweight geopolymer aggregate (ALGA) using LUSI (Sidoarjo mud) and alkaline activator as source materials. LUSI stands for LU-Lumpur and SI-Sidoarjo, meaning mud from Sidoarjo which erupted near the Banjarpanji-1 exploration well in Sidoarjo, East Java, Indonesia on 27 May 2006. The effect of NaOH molarity, LUSI mud/Alkaline activator (LM/AA) ratio, and Na2SiO3/NaOH ratio to the ALGA are investigated at a sintering temperature of 950 °C. The results show that the optimum NaOH molarity found in this study is 12 M due to the highest strength (lowest AIV value) of 15.79% with lower water absorption and specific gravity. The optimum LUSI mud/Alkaline activator (LM/AA) ratio of 1.7 and the Na2SiO3/NaOH ratio of 0.4 gives the highest strength with AIV value of 15.42% with specific gravity of 1.10 g/cm3 and water absorption of 4.7%. The major synthesized crystalline phases were identified as sodalite, quartz and albite. Scanning Electron Microscope (SEM) image showed more complete geopolymer matrix which contributes to highest strength of ALGA produced. PMID:26006238

UTJECAJ POZITIVNE DISKRIMINACIJE NA UKLJUČIVANJE ŽENA NA TRŽIŠTE RADA U REPUBLICI HRVATSKOJ

OpenAIRE

Dovranić, Dora; Katavić, Ivica

2016-01-01

U početku 21. stoljeća često se koristi pojam pozitivne (afirmativne) diskriminacije. Pozitivna diskriminacija mjera je koju poduzima vlada, neka organizacija ili korporacija s ciljem da se smanje nejednakosti u društvu nastale upravo diskriminacijom, na način da se pružaju određene povlastice onima koji su pretrpjeli neku vrstu diskriminacije. Nastala je u Sjedinjenim Američkim Državama na inicijativu predsjednika Kennedyja, Johnsona i Nixona. Diskriminacija, nažalost, ima mnogo oblika, a ne...

Heparinoides naturais isolados de rodofíceas (Halymenia sp. arribadas na costa cearense =Natural heparinoids isolated from Halymenia sp. (Rhodophyceaes delivery on the Ceará coast

Directory of Open Access Journals (Sweden)

José Ariévilo Gurgel Rodrigues

2010-07-01

Full Text Available A crescente carência de heparina (HEP motiva a busca por fontes alternativas de novos anticoagulantes naturais. Objetivou-se avaliar a atividade anticoagulante dos polissacarídeos sulfatados (PS isolados de uma rodofícea do gênero Halymenia, nativa do litoral cearense, Brasil. Os PS totais foram obtidos por digestões consecutivas com papaína em tampão acetato de sódio 0,1 M (pH 5,0, contendo cisteína 5 mM e EDTA 5 mM, seguidas por cromatografia de troca iônica em coluna de DEAE-celulose. As frações obtidas foram concentradas por liofilização e submetidas à eletroforese em gel de agarose a 0,5%. Os ensaios anticoagulantes foram realizados pelo tempo de tromboplastina parcial ativada (TTPA, usando-se plasma de coelho e uma curva padrão de HEP (100 UI mg-1. As extrações (53,96% mostraram diferenças marcantes durante o fracionamento e no grau de resolução dos PS. A espécie apresentou PS com atividade anticoagulante superior a HEP. O TTPA das frações modificou-se acentuadamente entre as extrações, expressando-se de maneira dose-dependente e sofrendo um acréscimo de 110,40 (1a extração para 143,10 UI mg-1 (3a extração. Os resultados sugerem que a atividadeanticoagulante dos PS isolados de Halymenia sp. foi promovida pela inibição da via intrínseca e/ou comum da cascata de coagulação. As modificações no TTPA possivelmente serão elucidadas pelos mecanismos de ação envolvidos na coagulação e caracterização estrutural desses compostos. Portanto, a rodofícea Halymenia sp. é uma boa fonte de heparinoides e sugerem-se estudos relacionados ao cultivo da espécie, em proteção aos bancos de algas.The increasing demand for heparin (HEP has led to a search for alternative sources of natural anticoagulants. This study aimed to evaluate the anticoagulant activity of sulfated polysaccharides (SP isolated from a Halymenia rhodophyceae genus native to the coast of Ceará, Brazil. Total SP were obtained by consecutive

Hydroxychloroquine augments early virological response to pegylated interferon plus ribavirin in genotype-4 chronic hepatitis C patients.

Science.gov (United States)

Helal, Gouda Kamel; Gad, Magdy Abdelmawgoud; Abd-Ellah, Mohamed Fahmy; Eid, Mahmoud Saied

2016-12-01

The therapeutic effect of pegylated interferon (peg-IFN) alfa-2a combined with ribavirin (RBV) on chronic hepatitis C Egyptian patients is low and further efforts are required to optimize this therapy for achievement of higher rates of virological response. This study aimed to evaluate the safety and efficacy of hydroxychloroquine (HCQ) in combination with pegylated interferon plus ribavirin on early virological response (EVR) in chronic hepatitis C Egyptian patients. Naïve 120 Egyptian patients with chronic hepatitis C virus infection were divided into two groups. Group 1 have administered the standard of care therapy (pegylated interferon alfa-2a plus ribavirin) for 12 weeks, (n = 60). Group 2 have administered hydroxychloroquine plus standard of care therapy for 12 weeks, (n = 60). Therapeutics included hydroxychloroquine (200 mg) oral twice daily, peginterferon alfa-2a (160 μg) subcutaneous once weekly and oral weight-based ribavirin (1000-1200 mg/day). Baseline characteristics were similar in the two groups. The percentage of early virological response was significantly more in patients given the triple therapy than in patients given the standard of care [54/60 (90%) vs. 43/60 (71.7%); P = 0.011; respectively]. Biochemical response at week 12 was also significantly higher in patients given the triple therapy compared with the standard of care [58/60 (96.7%) vs. 42/60 (70%); P hydroxychloroquine to pegylated interferon plus ribavirin improves the rate of early virological and biochemical responses in chronic hepatitis C Egyptian patients without an increase in adverse events. J. Med. Virol. 88:2170-2178, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

Reaction of metal oxides with molten mixtures NaPO3+NaCl

International Nuclear Information System (INIS)

Kovarskaya, E.N.; Mityakhina, V.S.; Rodionov, Yu.I.; Silin, M.Yu.

1988-01-01

By methods of solubility determination and paper chromatography it is shown, that Fe 2 O 3 solution in NaPO 3 +NaCl melts in the air relizes due to its chemical interaction with solvent resulting in formation of iron and sodium binary di- and monophosphates depending on melt-solvent initial composition, its attainment of equilibrium state and experiment temperature. It is established, that oxides increased solubility in melts with NaCl initial content ∼30 mol.% is specified by sodium tri- and tricyclophosphates presence in the melts. On this basis of NGR-spectroscopy data the presence of iron, europium, tin and sodium binary di- and monophosphates in some chloride-polyphosphate melts is confirmed

Crystal structure of polyphosphates NaCd(PO3)3 and NaMn(PO3)3

International Nuclear Information System (INIS)

Murashova, E.V.; Chudinova, N.N.

1997-01-01

Crystal structure of NaCd(PO 3 ) 3 (1) and NaMn(PO 3 ) 3 (2) isostructural polyphosphates was determined for twin samples. Rhombic lattice parameters of (1): a = 14.678, b = 14.669, c = 14.705 A, sp. gr. P2 1 2 1 2 1 , Z = 16. The structure of compounds is of frame type. Polyphosphate chain with repetition period of 24 PO 4 tetrahedrons contacts with NaO 6 and M 2 O 6 octahedrons by means of common oxygen vertices. Similarities and differences in structure of mentioned polyphosphates and earlier analyzed NaMg(PO 3 ) 3 polyphosphate are noted [ru

Abundância de oxigênio no aglomerado do bojo NGC 6553, com dados Gemini-Phoenix

Science.gov (United States)

Barbuy, B.; Melendez, J.; Bica, E.; Zoccali, M.; Ortolani, S.; Renzini, A.; Hill, V.

2003-08-01

Excesso de elementos-alfa com relação ao ferro dá indicação de enriquecimento por supernovas de tipo II. Foram observadas 5 estrelas gigantes do aglomerado globular do bojo NGC 6553, com o espectrógrafo Phoenix no Gemini-Sul. Foram obtidos espectros na banda H, na região centrada em 1.555 mm, com Dl = 75 Å, a uma resolução R = 50 000. A análise detalhada consistiu em determinar temperaturas efetivas e gravidades usando fotometria VIJK, e as linhas de FeI para determinar velocidades de microturbulência e metalicidade [Fe/H]. Linhas de CO e OH foram sintetizadas e comparadas aos espectros observados. A análise resulta em [Fe/H] = -0.2, [O/Fe] = +0.2, mostrando portanto excesso do elemento-a oxigênio.

Effect of growth in lithium on ouabain binding, Na-K-ATPase and Na and K transport in hela cells.

Science.gov (United States)

Boardman, L J; Hume, S P; Lamb, J F; Polson, J

1975-01-01

1. HeLa cells were grown for 24 hr in growth medium in which part of the Na was replaced with Li. Ion contents, cell volumes and numbers, Na-K-ATPase and specific ouabain binding were measured. In some experiments the Na efflux and net Na transport was also measured. 2. Growth in Li caused a rise in the specific ouabain binding and membrane Na-K-ATPase of these cells. The Li concentrations in the cells necessary to produce this effect ranged up to 50 mM. 3. It is suggested that Li, like Na, acts on the genetic material of the cells to cause the production of more Na pumps within the membrane. PMID:124350

Effects of EDTA-Na (Na ethylenediaminetetraacetate) upon the metabolism of radiostrontium and radioyttrium in mice

Energy Technology Data Exchange (ETDEWEB)

Kikuchi, T; Wakisaka, G; Kono, T; Hiroaki, A; Yamamasu, T; Sugawa, I

1954-01-01

The toxicity of EDTA-Na, inert Sr (NO/sub 3/)/sub 2/ and Ba (NO/sub 3/)/sub 2/ has been examined. Simultaneous injection of EDTA-Na showed no significant effect upon the distribution of radio-Sr in the bones of mice. The distribution of radio-Y in the bones of mice tended to decrease following the simultaneous subcutaneous injection of /sup 91/Y and EDTA-Na.

Prevalência de talassemias e hemoglobinas variantes no estado de Goiás, Brasil Prevalence of thalassemias and variant hemoglobins in the state of Goiás, Brazil

Directory of Open Access Journals (Sweden)

Paulo Roberto de Melo-Reis

2006-12-01

Full Text Available As anemias hereditárias, em especial as talassemias e hemoglobinas (Hb variantes, são as mais comuns das alterações genéticas humanas; sua freqüência na população brasileira é muito variável, dependendo dos grupos raciais formadores de cada região. O povoamento de Goiás, que teve início logo após o seu descobrimento, em 1726, motivado pela procura de ouro, foi composto principalmente por portugueses e escravos africanos, contexto que favoreceu a mestiçagem entre eles. Considerando que esses povos apresentam genes para as hemoglobinas anormais com freqüências variadas, é esperado que se encontrem essas alterações genéticas na nossa população. O objetivo deste trabalho foi avaliar a prevalência de talassemias e hemoglobinas variantes na população de Goiás. Para isso a casuística foi composta por 404 alunos participantes dos diversos cursos da Universidade Católica de Goiás (UCG, oriundos de 55 cidades do estado de Goiás. A prevalência de anemia hereditária por talassemias e hemoglobinas variantes em Goiás foi de 10,1%, cuja ordem decrescente foi a seguinte: talassemia alfa heterozigótica (5,2%, heterozigose para hemoglobina S (Hb AS (2,2%, heterozigose para hemoglobina C (Hb AC (1%, talassemia beta menor (0,7%, associação entre talassemia alfa e heterozigose para Hb S (0,5%, associação entre talassemia alfa e heterozigose para Hb C (0,3% e heterozigose para hemoglobina D (Hb AD (0,3%. Nenhum caso de homozigose foi encontrado no presente estudo. Este trabalho demonstrou a dispersão dos genes para Hb S, Hb C e Hb D, bem como de talassemias alfa e beta em uma população do estado de Goiás. Por essa razão, concluímos que é importante realizar programas com maior abrangência da população para estudo da epidemiologia das talassemias e hemoglobinas variantes no estado de Goiás.The hereditary anemias, especially the thalassemies and hemoglobinopathies are the most common human genetic abnormalities. Their

Novel aspects of Na+,K+-ATPase

OpenAIRE

Aizman, Oleg

2002-01-01

Na,K-ATPase, an integral membrane protein expressed in each eukaryotic cell, serves as the major determinant of intracellular ion composition. In the current study we investigated novel aspects of Na,K-ATPase function and regulation. It is well established that Na,K-ATPase activity is regulated by reversible phosphorylation. New findings in this study are: 1) the level of intracellular Ca 2. concentration determines the functional effects of PKA and PKC-mediated Na,K-ATP...

High-Performance Na-O2 Batteries Enabled by Oriented NaO2 Nanowires as Discharge Products.

Science.gov (United States)

Khajehbashi, S Mohammad B; Xu, Lin; Zhang, Guobin; Tan, Shuangshuang; Zhao, Yan; Wang, Lai-Sen; Li, Jiantao; Luo, Wen; Peng, Dong-Liang; Mai, Liqiang

2018-06-13

Na-O 2 batteries are emerging rechargeable batteries due to their high theoretical energy density and abundant resources, but they suffer from sluggish kinetics due to the formation of large-size discharge products with cubic or irregular particle shapes. Here, we report the unique growth of discharge products of NaO 2 nanowires inside Na-O 2 batteries that significantly boosts the performance of Na-O 2 batteries. For this purpose, a high-spin Co 3 O 4 electrocatalyst was synthesized via the high-temperature oxidation of pure cobalt nanoparticles in an external magnetic field. The discharge products of NaO 2 nanowires are 10-20 nm in diameter and ∼10 μm in length, characteristics that provide facile pathways for electron and ion transfer. With these nanowires, Na-O 2 batteries have surpassed 400 cycles with a fixed capacity of 1000 mA h g -1 , an ultra-low over-potential of ∼60 mV during charging, and near-zero over-potential during discharging. This strategy not only provides a unique way to control the morphology of discharge products to achieve high-performance Na-O 2 batteries but also opens up the opportunity to explore growing nanowires in novel conditions.

Insight to the Thermal Decomposition and Hydrogen Desorption Behaviors of NaNH2-NaBH4 Hydrogen Storage Composite.

Science.gov (United States)

Pei, Ziwei; Bai, Ying; Wang, Yue; Wu, Feng; Wu, Chuan

2017-09-20

The lightweight compound material NaNH 2 -NaBH 4 is regarded as a promising hydrogen storage composite due to the high hydrogen density. Mechanical ball milling was employed to synthesize the composite NaNH 2 -NaBH 4 (2/1 molar ratio), and the samples were investigated utilizing thermogravimetric-differential thermal analysis-mass spectroscopy (TG-DTA-MS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) analyses. The full-spectrum test (range of the ratio of mass to charge: 0-200) shows that the released gaseous species contain H 2 , NH 3 , B 2 H 6 , and N 2 in the heating process from room temperature to 400 °C, and possibly the impurity gas B 6 H 12 also exists. The TG/DTA analyses show that the composite NaNH 2 -NaBH 4 (2/1 molar ratio) is conductive to generate hydrogen so that the dehydrogenation process can be finished before 400 °C. Moreover, the thermal decomposition process from 200 to 400 °C involves two-step dehydrogenation reactions: (1) Na 3 (NH 2 ) 2 BH 4 hydride decomposes into Na 3 BN 2 and H 2 (200-350 °C); (2) remaining Na 3 (NH 2 ) 2 BH 4 reacts with NaBH 4 and Na 3 BN 2 , generating Na, BN, NH 3 , N 2 , and H 2 (350-400 °C). The better mechanism understanding of the thermal decomposition pathway lays a foundation for tailoring the hydrogen storage performance of the composite complex hydrides system.

Distinct pH dependencies of Na+/K+ selectivity at the two faces of Na,K-ATPase.

Science.gov (United States)

Cornelius, Flemming; Tsunekawa, Naoki; Toyoshima, Chikashi

2018-02-09

The sodium pump (Na,K-ATPase) in animal cells is vital for actively maintaining ATP hydrolysis-powered Na + and K + electrochemical gradients across the cell membrane. These ion gradients drive co- and countertransport and are critical for establishing the membrane potential. It has been an enigma how Na,K-ATPase discriminates between Na + and K + , despite the pumped ion on each side being at a lower concentration than the other ion. Recent crystal structures of analogs of the intermediate conformations E2·Pi·2K + and Na + -bound E1∼P·ADP suggest that the dimensions of the respective binding sites in Na,K-ATPase are crucial in determining its selectivity. Here, we found that the selectivity at each membrane face is pH-dependent and that this dependence is unique for each face. Most notable was a strong increase in the specific affinity for K + at the extracellular face ( i.e. E2 conformation) as the pH is lowered from 7.5 to 5. We also observed a smaller increase in affinity for K + on the cytoplasmic side (E1 conformation), which reduced the selectivity for Na + Theoretical analysis of the p K a values of ion-coordinating acidic amino acid residues suggested that the face-specific pH dependences and Na + /K + selectivities may arise from the protonation or ionization of key residues. The increase in K + selectivity at low pH on the cytoplasmic face, for instance, appeared to be associated with Asp 808 protonation. We conclude that changes in the ionization state of coordinating residues in Na,K-ATPase could contribute to altering face-specific ion selectivity. © 2018 by The American Society for Biochemistry and Molecular Biology, Inc.

OGLAŠEVANJE NA FACEBOOKU

OpenAIRE

Renko, Katica

2013-01-01

V magistrskem delu smo se seznanili z oglaševanjem na Facebooku. S pregledom gradiva, ki nam je ponujeno, smo preverili pogoje, načine, oblike … oglaševanja, ki pa smo jih s pregledom situacije v praksi preverili in dopolnili. Ob zaključku dela lahko rečemo, da je oglaševanje preko FB enostavnejše, cenejše, dostopnejše, omogoča nam ažurnost in samo komunikacijo z naslovniki, ki so potencialni kupci naših ponudb. Množični mediji nam omogočajo mnogotero obliko oglaševanja. Izbrana oblika ponuja...

Laser-induced ionization of Na vapor

International Nuclear Information System (INIS)

Wu, R.C.Y.; Judge, D.L.; Roussel, F.; Carre, B.; Breger, P.; Spiess, G.

1982-01-01

The production of Na 2 + ions by off-resonant laser excitation in the 5800-6200A region mainly results from two-photon absorption by the Na 2 molecule to highly excited gerade states followed by (a) direct ionization by absorbing a third photon or (b) coupling to the molecular Na 2 D 1 PIμ Rydberg state which is subsequently ionized by absorbing a third photon. This mechanism, i.e., a two-photon resonance three photon ionization process, explains a recent experimental observation of Roussel et al. It is suggested that the very same mechanism is also responsible for a similar observation reported by Polak-Dingels et al in their work using two crossed Na beams. In the latter two studies the laser-induced associative ionization processes were reported to be responsible for producing the Na 2 + ion. From the ratio of molecular to atomic concentration in the crossed beam experiment of Polak-Dingels et al we estimate that the cross section for producing Na 2 + through laser-induced associative ionization is at least four orders of magnitude smaller than ionization through the two-photon resonance three photon ionization process in Na 2 molecules

Characterization and antibacterial activity of silver exchanged regenerated NaY zeolite from surfactant-modified NaY zeolite

Energy Technology Data Exchange (ETDEWEB)

Salim, Mashitah Mad; Malek, Nik Ahmad Nizam Nik, E-mail: niknizam@fbb.utm.my

2016-02-01

The antibacterial activity of regenerated NaY zeolite (thermal treatment from cetyltrimethyl ammonium bromide (CTAB)-modified NaY zeolite and pretreatment with Na ions) loaded with silver ions were examined using the broth dilution minimum inhibitory concentration (MIC) method against Escherichia coli (E. coli ATCC 11229) and Staphylococcus aureus (S. aureus ATCC 6538). X-ray diffraction (XRD), attenuated total reflectance–Fourier transform infrared (ATR–FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) and chemical elemental analyses were used to characterize the regenerated NaY and AgY zeolites. The XRD patterns indicated that the calcination and addition of silver ions on regenerated NaY zeolite did not affect the structure of the regenerated NaY zeolite as the characteristic peaks of the NaY zeolite were retained, and no new peaks were observed. The regenerated AgY zeolite showed good antibacterial activity against both bacteria strains in distilled water, and the antibacterial activity of the samples increased with increasing Ag loaded on the regenerated AgY zeolite; the regenerated AgY zeolite was more effective against E. coli than S. aureus. However, the antibacterial activity of the regenerated AgY was not effective in saline solution for both bacteria. The study showed that CTAB-modified NaY zeolite materials could be regenerated to NaY zeolite using thermal treatment (550 °C, 5 h) and this material has excellent performance as an antibacterial agent after silver ions loading. - Highlights: • Thermal treatment was used to regenerate surfactant modified zeolite. • The regenerated NaY zeolite formed was added with different silver loadings. • Regenerated AgY zeolite was tested for antibacterial activity on E. coli and S. aureus. • The antibacterial activity increased with increased of the amount of silver loadings. • The zeolite structure did not change with thermal and modification

Characterization and antibacterial activity of silver exchanged regenerated NaY zeolite from surfactant-modified NaY zeolite

International Nuclear Information System (INIS)

Salim, Mashitah Mad; Malek, Nik Ahmad Nizam Nik

2016-01-01

The antibacterial activity of regenerated NaY zeolite (thermal treatment from cetyltrimethyl ammonium bromide (CTAB)-modified NaY zeolite and pretreatment with Na ions) loaded with silver ions were examined using the broth dilution minimum inhibitory concentration (MIC) method against Escherichia coli (E. coli ATCC 11229) and Staphylococcus aureus (S. aureus ATCC 6538). X-ray diffraction (XRD), attenuated total reflectance–Fourier transform infrared (ATR–FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) and chemical elemental analyses were used to characterize the regenerated NaY and AgY zeolites. The XRD patterns indicated that the calcination and addition of silver ions on regenerated NaY zeolite did not affect the structure of the regenerated NaY zeolite as the characteristic peaks of the NaY zeolite were retained, and no new peaks were observed. The regenerated AgY zeolite showed good antibacterial activity against both bacteria strains in distilled water, and the antibacterial activity of the samples increased with increasing Ag loaded on the regenerated AgY zeolite; the regenerated AgY zeolite was more effective against E. coli than S. aureus. However, the antibacterial activity of the regenerated AgY was not effective in saline solution for both bacteria. The study showed that CTAB-modified NaY zeolite materials could be regenerated to NaY zeolite using thermal treatment (550 °C, 5 h) and this material has excellent performance as an antibacterial agent after silver ions loading. - Highlights: • Thermal treatment was used to regenerate surfactant modified zeolite. • The regenerated NaY zeolite formed was added with different silver loadings. • Regenerated AgY zeolite was tested for antibacterial activity on E. coli and S. aureus. • The antibacterial activity increased with increased of the amount of silver loadings. • The zeolite structure did not change with thermal and modification

Salicylic acid degradation from aqueous solutions using Pseudomonas fluorescens HK44: parameters studies and application tools Degradação de ácido salicílico presente em soluções sintéticas utilizando Pseudomonas fluorescens HK44

Directory of Open Access Journals (Sweden)

Tatyane R. Silva

2007-03-01

Full Text Available The optimal conditions for salicylic acid biodegradation by Pseudomonas fluorescens HK44 were determined in this study with the intention to create a microbial sensor. Kinetic experiments permitted a definition of 60 and 30min the time needed to achieve the maximum degradation of salicylic acid presented in a medium with and without yeast extract, respectively. The degradation in medium without yeast extract and the quantification by spectrophotometry 230 nm were selected to be used in further tests. The use of preactivated cells or on the exponential growth phase showed better salicylic acid degradation percentages when compared to nonactivated cells or on the stationary growth state. Finally, the best cellular concentration used on the salicylic acid degradation was 0,1 g.L-1. Strain HK44 shows to be capable of degrade salicylic acid presented in simple aqueous systems, making this strain a promising tool for the application on a luminescent microbial sensor.Com a intenção de criar um sensor microbiano, as condições ótimas para a biodegradação de ácido salicílico por Pseudomonas fluorescens HK44 foram determinadas neste estudo. Os experimentos cinéticos permitiram a definição dos tempos de 60 e 30 minutos como necessários para atingir a máxima degradação de ácido salicílico presente em meio com ou sem extrato de lêvedo, respectivamente. A degradação no meio sem extrato de lêvedo e a quantificação através de espectrofotometria 230 nm foram selecionadas para serem utilizadas em testes posteriores. O uso de células pré-ativadas ou na fase exponencial de crescimento apresentou melhores porcentagens de degradação de ácido salicílico quando comparadas a células não-ativadas ou no estado estacionário de crescimento. Além disso, a melhor concentração celular utilizada nessa degradação foi 0,1 g.L¹. A cepa HK44 parece ser capaz de degradar o ácido salicílico presente em sistemas aquosos simples, tornando este

Carbon dioxide sequestration using NaHSO4 and NaOH: A dissolution and carbonation optimisation study.

Science.gov (United States)

Sanna, Aimaro; Steel, Luc; Maroto-Valer, M Mercedes

2017-03-15

The use of NaHSO 4 to leach out Mg fromlizardite-rich serpentinite (in form of MgSO 4 ) and the carbonation of CO 2 (captured in form of Na 2 CO 3 using NaOH) to form MgCO 3 and Na 2 SO 4 was investigated. Unlike ammonium sulphate, sodium sulphate can be separated via precipitation during the recycling step avoiding energy intensive evaporation process required in NH 4 -based processes. To determine the effectiveness of the NaHSO 4 /NaOH process when applied to lizardite, the optimisation of the dissolution and carbonation steps were performed using a UK lizardite-rich serpentine. Temperature, solid/liquid ratio, particle size, concentration and molar ratio were evaluated. An optimal dissolution efficiency of 69.6% was achieved over 3 h at 100 °C using 1.4 M sodium bisulphate and 50 g/l serpentine with particle size 75-150 μm. An optimal carbonation efficiency of 95.4% was achieved over 30 min at 90 °C and 1:1 magnesium:sodium carbonate molar ratio using non-synthesised solution. The CO 2 sequestration capacity was 223.6 g carbon dioxide/kg serpentine (66.4% in terms of Mg bonded to hydromagnesite), which is comparable with those obtained using ammonium based processes. Therefore, lizardite-rich serpentinites represent a valuable resource for the NaHSO 4 /NaOH based pH swing mineralisation process. Copyright © 2016 Elsevier Ltd. All rights reserved.

Characterization and antibacterial activity of silver exchanged regenerated NaY zeolite from surfactant-modified NaY zeolite.

Science.gov (United States)

Salim, Mashitah Mad; Malek, Nik Ahmad Nizam Nik

2016-02-01

The antibacterial activity of regenerated NaY zeolite (thermal treatment from cetyltrimethyl ammonium bromide (CTAB)-modified NaY zeolite and pretreatment with Na ions) loaded with silver ions were examined using the broth dilution minimum inhibitory concentration (MIC) method against Escherichia coli (E. coli ATCC 11229) and Staphylococcus aureus (S. aureus ATCC 6538). X-ray diffraction (XRD), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) and chemical elemental analyses were used to characterize the regenerated NaY and AgY zeolites. The XRD patterns indicated that the calcination and addition of silver ions on regenerated NaY zeolite did not affect the structure of the regenerated NaY zeolite as the characteristic peaks of the NaY zeolite were retained, and no new peaks were observed. The regenerated AgY zeolite showed good antibacterial activity against both bacteria strains in distilled water, and the antibacterial activity of the samples increased with increasing Ag loaded on the regenerated AgY zeolite; the regenerated AgY zeolite was more effective against E. coli than S. aureus. However, the antibacterial activity of the regenerated AgY was not effective in saline solution for both bacteria. The study showed that CTAB-modified NaY zeolite materials could be regenerated to NaY zeolite using thermal treatment (550°C, 5h) and this material has excellent performance as an antibacterial agent after silver ions loading. Copyright © 2015 Elsevier B.V. All rights reserved.

Composition and thermochemistry of the equilibrium vapour of the systems NaI-FeI2 and NaI-PbI2

International Nuclear Information System (INIS)

Hilpert, K.; Gerads, H.; Koberts, D.; Miller, M.

1987-01-01

The vaporization of NaI/FeI 2 and NaI/PbI 2 samples of equimolar composition was investigated was investigated in the temperature ranges between 574 to 683 K and 562 to 669 K, respectively, by using the mass spectrometric Knudsen effusion method. The gaseous species I, I 2 , NaI, (NaI) 2 , FeI 2 , (FeI 2 ) 2 , FeI 3 , NaFeI 3 , and Na 2 FeI 4 (NaI-FeI 2 system) as well as NaI, (NaI) 2 , PbI 2 , (PbI 2 ) 2 , and NaPbI 3 (NaI-PbI 2 system) are present in the equilibrium vapours. The equilibrium partial pressures of these species were determined with the exception of I, I 2 , and FeI 3 . Enthalpies and entropies of dissociation resulted for the reactions NaFeI 3 (g) ↔ NaI(g)+FeI 2 (g) (1), Na 2 FeI 4 (g) ↔ 2NaI(g)+FeI 2 (g) and (2) NaPbI 3 (g) ↔ NaI(g)+PbI 2 (g) (3) as Δ d H 298 0 (Eq. (1)) = 184±5 kJ mol -1 , Δ d S 298 0 (Eq. (1)) = 143±8 J mol -1 K -1 ; Δ d H 298 0 (Eq. (2)) = 333±9 kJ mol -1 , Δ d S 298 0 (Eq. (2)) = 274±14 J mol -1 K -1 ; and Δ d H 298 0 (Eq. (3)) = 168±5 kJ mol -1 , Δ d S 298 0 (Eq. (3)) = 151±9 J mol -1 K -1 . Equilibrium constants for these reactions are additionally given. The pressures of NaFeI 3 (g) and NaPbI 3 (g) as all as their enthalpies of dissociation are discussed with respect to their significance of semi empirical rules. (orig.)