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Sample records for alfa 1 antitripsina

  1. Deficiência de alfa-1 antitripsina: diagnóstico e tratamento Alpha-1 antitrypsin deficiency: diagnosis and treatment

    OpenAIRE

    Aquiles A Camelier; Daniel Hugo Winter; José Roberto Jardim; Carlos Eduardo Galvão Barboza; Alberto Cukier; Marc Miravitlles

    2008-01-01

    A deficiência de alfa-1 antitripsina é um distúrbio genético de descoberta recente e que ocorre com freqüência comparável à da fibrose cística. Resulta de diferentes mutações no gene SERPINA1 e tem diversas implicações clínicas. A alfa-1 antitripsina é produzida principalmente no fígado e atua como uma antiprotease. Tem como principal função inativar a elastase neutrofílica, impedindo a ocorrência de dano tecidual. A mutação mais freqüentemente relacionada à doença clínica é o alelo Z, que de...

  2. Deficiência de alfa-1-antitripsina na doença pulmonar obstrutiva crónica

    OpenAIRE

    Cardoso, Patrícia Neves

    2009-01-01

    Introdução – A deficiência de alfa-1-antitripsina é uma das alterações genéticas graves encontradas por todo o mundo. Porém, permanece subdiagnosticada, apesar da forte predisposição à doença pulmonar obstrutiva crónica que condiciona. Objectivos – Os objectivos deste artigo de revisão incluem o estudo e avaliação das implicações da deficiência de alfa-1-antitripsina em indivíduos com doença pulmonar obstrutiva crónica, a apresentação dos esquemas de diagnóstico e terapêuticos mais utiliza...

  3. Tratamento de reposição na deficiência de alfa-1-antitripsina em doentes com enfisema pulmonar

    OpenAIRE

    Pinho, Crisbety Marlene Soares de

    2013-01-01

    A doença pulmonar obstrutiva crónica é uma das principais causas de incapacidade respiratória e a quarta causa de morte em todo o Mundo, sendo mais frequente no sexo masculino. A exposição ao fumo do tabaco é considerada o principal fator de risco ambiental, no entanto, existem outros fatores igualmente importantes como, por exemplo, a deficiência de alfa-1-antitripsina, a única desordem genética conhecida associada a esta patologia até aos dias de hoje. Esta enzima é maioritariamente produzi...

  4. Deficiência de alfa-1 antitripsina: diagnóstico e tratamento Alpha-1 antitrypsin deficiency: diagnosis and treatment

    Directory of Open Access Journals (Sweden)

    Aquiles A Camelier

    2008-07-01

    Full Text Available A deficiência de alfa-1 antitripsina é um distúrbio genético de descoberta recente e que ocorre com freqüência comparável à da fibrose cística. Resulta de diferentes mutações no gene SERPINA1 e tem diversas implicações clínicas. A alfa-1 antitripsina é produzida principalmente no fígado e atua como uma antiprotease. Tem como principal função inativar a elastase neutrofílica, impedindo a ocorrência de dano tecidual. A mutação mais freqüentemente relacionada à doença clínica é o alelo Z, que determina polimerização e acúmulo dentro dos hepatócitos. O acúmulo e a conseqüente redução dos níveis séricos de alfa-1 antitripsina determinam, respectivamente, doença hepática e pulmonar, sendo que esta se manifesta principalmente sob a forma de enfisema de aparecimento precoce, habitualmente com predomínio basal. O diagnóstico envolve a detecção de níveis séricos reduzidos de alfa-1 antitripsina e a confirmação fenotípica. Além do tratamento usual para doença pulmonar obstrutiva crônica, existe atualmente uma terapia específica com infusão de concentrados de alfa-1 antitripsina. Essa terapia de reposição, aparentemente segura, ainda não teve a eficácia clínica definitivamente comprovada, e o custo-efetividade também é um tema controverso e ainda pouco abordado. Apesar da sua importância, não existem dados epidemiológicos brasileiros a respeito da prevalência da doença ou da freqüência de ocorrência dos alelos deficientes. O subdiagnóstico também tem sido uma importante limitação tanto para o estudo da doença quanto para o tratamento adequado dos pacientes. Espera-se que a criação do Registro Internacional de Alfa-1 venha a resolver essas e outras importantes questões.Alpha-1 antitrypsin deficiency is a recently identified genetic disease that occurs almost as frequently as cystic fibrosis. It is caused by various mutations in the SERPINA1 gene, and has numerous clinical

  5. Registro Español de pacientes con déficit de alfa-1 antitripsina: evaluación de la base de datos y estudio de la población de pacientes incluida

    OpenAIRE

    Lara Gallego, Beatriz

    2014-01-01

    Introducción: La alfa-1 antitripsina (AAT) es una proteina con capacidad antiproteasa cuyo principal órgano diana es el pulmón. Las variantes proteicas deficitarias más frecuentes son las denominadas Z y S que presentan tendencia a formar polímeros hepáticos por lo que su concentración plasmática es muy pequeña. En el déficit de AAT la ausencia de la proteina en el pulmón predispone al desarrollo de enfisema. En España, según los estudios de prevalencia, se esperarían unos 160....

  6. Evaluación del efecto de la ingesta de una alta carga de ácidos grasos saturados sobre los niveles séricos de la proteína C reactiva, alfa1-antitripsina, fibrinógeno y alfa1-glicoproteína ácida en mujeres obesas Effect of a high saturated fatty acids load on serum concentrations of C-reactive protein, alpha1-antitrypsin, fibrinogen and alpha1-acid glycoprotein in obese women

    Directory of Open Access Journals (Sweden)

    M.ª M. Ramírez Alvarado

    2010-02-01

    Full Text Available La obesidad está asociada con un estado inflamatorio. La proteína C reactiva (PCR y las proteínas plasmáticas sensibles a inflamación (ISPs son marcadores de inflamación. El proceso proinflamatorio podría ser influenciado por la ingesta de altas cantidades de ácidos grasos saturados. Objetivo: evaluar el efecto de la ingesta de una alta carga de ácidos grasos saturados sobre los niveles séricos de PCR y de ISPs (alfa1-antitripsina, alfa1-glicoproteína ácida y fibrinógeno en mujeres obesas. Metodología: la población estuvo conformada por 15 mujeres obesas (edad = 31,7 ± 4,5 años, IMC = 37,9 ± 7,3 kg/m² y 15 mujeres normopeso (edad = 30,6 ± 4,6 años, IMC = 20,6 ± 2,6 kg/m². Los sujetos en ayuno se sometieron a la ingesta de 75 g de grasa (100% ácidos grasos saturados, 0% de colesterol, 5 g de carbohidratos y 6 g de proteína por m² de superficie corporal. Se midió los niveles en ayuno y postprandiales de PCR y de ISPs. Los parámetros antropométricos y bioquímicos se midieron en ambos grupos. Resultados: las mujeres obesas presentaron mayores niveles séricos de PCR (p = 0,013 y de fibrinógeno (p = 0,04 que las mujeres normopeso. Los niveles séricos de PCR y fibrinógeno se correlacionaron positivamente con el índice de masa corporal (IMC en el grupo obeso. No se observó diferencias en los niveles de alfa1-antitripsina (p = 0,40 ni de alfa1-glicoproteína ácida (p = 0,28 en ayuno en mujeres obesas en comparación con mujeres normopeso. Los niveles séricos de PCR, alfa1-antitripsina, alfa1-glicoproteína ácida y fibrinógeno no cambiaron luego de la ingestión de la cargalipídica (p = > 0,05 diferencia con el nivel preprandial. Conclusión: la ingesta de una alta carga de ácidos grasos saturados no tiene ningún efecto sobre los niveles séricos de PCR, alfa1-antitripsina, alfa1-glicoproteína ácida y fibrinógeno. Los niveles séricos de alfa1-antitripsina y alfa1-glicoproteína ácida no están incrementados

  7. Avaliação da concentração de alfa 1-antitripsina e da presença dos alelos S e Z em uma população de indivíduos sintomáticos respiratórios crônicos Determination of alpha 1-antitrypsin levels and of the presence of S and Z alleles in a population of patients with chronic respiratory symptoms

    OpenAIRE

    Heliane Guerra Serra; Carmen Sílvia Bertuzzo; Mônica Corso Pereira; Cláudio Lúcio Rossi; Walter Pinto Júnior; Ilma Aparecida Paschoal

    2008-01-01

    OBJETIVO: Determinar a concentração de alfa 1-antitripsina (AAT) e a prevalência dos alelos S e Z em indivíduos sintomáticos respiratórios crônicos. MÉTODOS: Pacientes com tosse crônica e dispnéia foram submetidos à avaliação clínica, espirometria, tomografia computadorizada de tórax, dosagem de AAT por nefelometria e pesquisa das mutações S e Z por reação em cadeia da polimerase. Foram consideradas como variáveis dependentes a concentração de AAT e o tabagismo. RESULTADOS: Dos 89 pacientes i...

  8. Diagnóstico tardío de déficit de α-1-antitripsina

    Directory of Open Access Journals (Sweden)

    P. Rodríguez

    2011-04-01

    Full Text Available El déficit de alfa-1 antitripsina (D-AAT es una enfermedad genética, relativamente frecuente en poblaciones de origen europeo. Frecuentemente está infradiagnosticada, con largos retrasos entre el inicio de los síntomas respiratorios y el diagnóstico definitivo. El D-AAT puede diagnosticarse con la determinación de los niveles séricos de esta proteína, y, cuando están por debajo del rango normal debe ser confirmado con el fenotipo. El seguimiento correcto de la progresión de la enfermedad debe realizarse en todos los casos, ya que se asocia con mejor pronóstico. Presentamos el caso de una mujer de 69 años, con infecciones respiratorias de repetición y sintomatología asmática. Los niveles de AAT estaban por debajo de la normalidad, confirmándose posteriormente el fenotipo ZZ. El retraso diagnóstico de nuestra paciente parece reforzar la necesidad de alertar a los médicos sobre esta enfermedad, frecuentemente asociada con síntomas de asma o EPOC.

  9. Avaliação da concentração de alfa 1-antitripsina e da presença dos alelos S e Z em uma população de indivíduos sintomáticos respiratórios crônicos Determination of alpha 1-antitrypsin levels and of the presence of S and Z alleles in a population of patients with chronic respiratory symptoms

    Directory of Open Access Journals (Sweden)

    Heliane Guerra Serra

    2008-12-01

    Full Text Available OBJETIVO: Determinar a concentração de alfa 1-antitripsina (AAT e a prevalência dos alelos S e Z em indivíduos sintomáticos respiratórios crônicos. MÉTODOS: Pacientes com tosse crônica e dispnéia foram submetidos à avaliação clínica, espirometria, tomografia computadorizada de tórax, dosagem de AAT por nefelometria e pesquisa das mutações S e Z por reação em cadeia da polimerase. Foram consideradas como variáveis dependentes a concentração de AAT e o tabagismo. RESULTADOS: Dos 89 pacientes incluídos no estudo (44 mulheres; idade média, 51,3 ± 18,2 anos, os alelos S e Z foram detectados em 33,3% e 5,7%, respectivamente, com freqüência gênica dos alelos S e Z de 0,16 e 0,028. Dois pacientes tinham genótipo SZ (AAT 141 mg/dL (normal, Grupo 2, n = 57. A freqüência de fumantes foi igual nos dois grupos, com carga tabágica maior no Grupo 2. O alelo S estava presente em 13 e 14 pacientes dos Grupos 1 e 2, respectivamente, enquanto que o alelo Z estava presente em 2 e 1 paciente dos mesmos grupos. Não houve diferença nos testes de função pulmonar, nem na freqüência de bronquiectasias ou enfisema entre os dois grupos. Os valores espirométricos e as concentrações de AAT foram similares entre fumantes e não-fumantes. Bronquiectasias foram mais freqüentes entre os não fumantes, e enfisema foi mais freqüente entre os fumantes. CONCLUSÕES: Trinta pacientes apresentaram níveis de AAT abaixo da média esperada para os genótipos MM e MS, e este fato não pode ser explicado por uma freqüência maior dos alelos S e Z.OBJECTIVE: To determine the levels of alpha-1 antitrypsin (AAT and the presence of S and Z alleles in patients with chronic respiratory symptoms. METHODS: Patients with chronic cough and dyspnea were submitted to clinical evaluation, pulmonary function tests, high-resolution computed tomography, nephelometric determination of AAT and determination of S and Z alleles by polymerase chain reaction. Smoking

  10. Diagnóstico tardío de déficit de α-1-antitripsina Delayed diagnosis of alpha-1 antitrypsin deficiency

    Directory of Open Access Journals (Sweden)

    P. Rodríguez

    2011-04-01

    Full Text Available El déficit de alfa-1 antitripsina (D-AAT es una enfermedad genética, relativamente frecuente en poblaciones de origen europeo. Frecuentemente está infradiagnosticada, con largos retrasos entre el inicio de los síntomas respiratorios y el diagnóstico definitivo. El D-AAT puede diagnosticarse con la determinación de los niveles séricos de esta proteína, y, cuando están por debajo del rango normal debe ser confirmado con el fenotipo. El seguimiento correcto de la progresión de la enfermedad debe realizarse en todos los casos, ya que se asocia con mejor pronóstico. Presentamos el caso de una mujer de 69 años, con infecciones respiratorias de repetición y sintomatología asmática. Los niveles de AAT estaban por debajo de la normalidad, confirmándose posteriormente el fenotipo ZZ. El retraso diagnóstico de nuestra paciente parece reforzar la necesidad de alertar a los médicos sobre esta enfermedad, frecuentemente asociada con síntomas de asma o EPOC.Alpha-1 antitrypsin deficiency (AAT-D is a genetic disease, relatively common in populations of European ancestry. AAT-D remains undiagnosed in many patients, and there are often long delays between the onset of respiratory symptoms and diagnosis. AAT-D can be readily diagnosed by measurement of the serum or plasma protein level, which should be confirmed by assessing the genotype or protein phenotype when AAT levels are below the normal range. Close monitoring for the development or progression of lung disease or liver disease is required, and can improve the prognosis. We show the case of a 69 year old woman with recurrent respiratory infections and asthma symptoms. The serum levels of AAT were below the normal range, and ZZ genotype was confirmed. The delayed diagnosis of our patient seems to emphasize the need to remind the doctors about AAT-D, frequently associated with asthma or COPD symptoms.

  11. Importancia del fenotipo de la a1-antitripsina en las manifestaciones clínicas y en el pronóstico de las enfermedades sistémicas autoinmunes

    OpenAIRE

    Jiménez Moreno, Francesc Xavier

    2001-01-01

    El déficit de a1 -antitripsina (a-1-AT) es una enfermedad hereditaria relativamente frecuente que se caracteriza clásicamente por una reducción de los valores séricos de a-1-AT, y por la presencia de enfisema pulmonar y hepatopatía.Las mutaciones del gen de la a-1-AT provocan una incapacidad de sintetizar y secretar cantidades normales de a-1-AT. Cuando los valores séricos son menores de 50 mg/dl la a-1-AT sintetizada es insuficiente para proteger el alvéolo de la elastasa, provocando una pr...

  12. Instilación intratraqueal de elastasa en la rata: Cambios electroforéticos de la α1-AT-antitripsina en el lavado broncoalveolar Intratracheal instillation of elastase in the rat: Electrophoretical changes of alphal-antitrypsin in bronchoalveolar lavage fluid

    Directory of Open Access Journals (Sweden)

    ANDREA VECCHIOLA C

    2008-03-01

    Full Text Available Introducción: la antiproteasa alfa 1-antitripsina ( α1-AT constituye el principal inhibidor endógeno de la elastasa instilada por vía intratraqueal en modelos experimentales. Objetivo: Evaluar mediante electroforesis e inmunodetección por western blot, las distintas formas en que se encuentra la α1-AT en el lavado broncoalveolar (IBA de ratas Sprague Dawley después de la instilación de elastasa, con la hipótesis de que el aumento en la actividad antielastasa previamente encontrada se acompaña de niveles altos de α1-AT activa. Resultados: En las primeras horas post-elastasa la concentración de α1-AT en el IBA aumenta más de 7 veces, debido al aumento de la permeabilidad alvéolo-capilar, encontrándose tanto como proteína nativa (~ 52 kDa, como parte de complejos de mayor tamaño (> 75 kDa y > 100 kDa y como producto de proteólisis (α1-AT inactiva formando complejos, el aumento de la permeabilidad alvéolo-capilar contribuye a mantener niveles altos de α1-AT activa. Estos resultados podrían ser extrapolables a distintos procesos inflamatorios pulmonaresIntroduction: Endogenous alphal-antitrypsin ( α1-AT is the main inhibitor of the intratracheally instilled elastase in experimental animals. Objective: To evaluate by electrophoresis and immunodetection using western blot analysis, the different forms of α1-AT in bronchoalveolar lavage fluid (BALF of Sprague Dawley rats after intratracheal instillation of elastase, with the hypothesis that the previously observed increment in antielastase activity is due to high levels of active α1-AT. Results: In the first hours after elastase instillation the concentration of α1-AT increases more than seven times due to an increase in alveolar-capillary permeability. α1-AT in BAIF is found as the native protein (~ 52 kDa, as complexes of different molecular sizes (> 75 kDa and > 100 kDa and as a proteolytic product (α1-AT in the inactive form as part of different complexes, the increase

  13. Daclatasvir plus peginterferon alfa and ribavirin for treatment-naive chronic hepatitis C genotype 1 or 4 infection

    DEFF Research Database (Denmark)

    Hézode, Christophe; Hirschfield, Gideon M; Ghesquiere, Wayne;

    2015-01-01

    OBJECTIVE: To evaluate the safety and efficacy of daclatasvir, an HCV NS5A inhibitor with pangenotypic activity, administered with peginterferon-alfa-2a/ribavirin. DESIGN: In this Phase 2b double-blind, placebo-controlled study, treatment-naive adults with HCV genotype 1 (N=365) or 4 (N=30......) infection were randomly assigned (2:2:1) to daclatasvir 20 mg or 60 mg, or placebo once daily plus weekly peginterferon-alfa-2a and twice-daily ribavirin. Daclatasvir recipients achieving protocol-defined response (PDR; HCV-RNA... rerandomised at Week 12 to continue daclatasvir/peginterferon-alfa-2a/ribavirin for 24 weeks total duration or to placebo/peginterferon-alfa-2a/ribavirin for another 12 weeks. Patients without PDR and placebo patients continued peginterferon-alfa/ribavirin through Week 48. Primary efficacy endpoints were...

  14. Determinação sérica de haptoglobina, ceruloplasmina, α1-glicoproteína ácida, transferrina e α1-antitripsina, em equinos com cólica Determination of serum haptoglobin, ceruloplasmin, α1-acid glycoprotein, transferrin and α1-antitrypsin in colic horses

    Directory of Open Access Journals (Sweden)

    Paula Alessandra Di Filippo

    2011-12-01

    Full Text Available Foram examinados 46 equinos adultos, 6 hígidos (G1 e 40 com cólica, submetidos à laparotomia. Vinte apresentavam lesões no intestino grosso (G2 e 20 no intestino delgado (G3. Avaliaram-se os teores séricos das proteínas de fase aguda: haptoglobina, ceruloplasmina, antitripsina, transferrina e glicoproteína ácida, antes e até sete dias após a laparotomia. Após centrifugação e fracionamento das amostras, as proteínas de fase aguda foram separadas por eletroforese em gel de poliacrilamida contendo SDS-PAGE, e suas concentrações determinadas por densitometria computadorizada. Constatou-se elevação dos valores das proteínas de fase aguda nos animais com cólica, antes e após laparotomia, porém com valores mais elevados, precoces e persistentes nos animais do G3. Os resultados deveram-se ao processo inflamatório intestinal, desencadeado pela lesão entérica e indicam que o proteinograma sérico pode auxiliar na identificação do segmento intestinal obstruído e, consequentemente, na elaboração do prognóstico de equinos com cólica.Forty six equines were examined, 6 were healthy (G1 and 40 with colic, submitted to laparotomy. Twenty were showing lesions on the large intestine (G2 and 20 lesions on the small intestine (G3. The serum concentrations of acute phase proteins: haptoglobin, ceruloplasmin, antitrypsin, transferrin, and - acid glycoprotein before and until 7 days after laparotomy. After centrifugation and fractioning of the samples, the acute phase proteins were individualized by electrophoresis on polyacrylamide gel containing SDS-PAGE, and the concentrations were determined by computerized densitometry. The increase was verified on the levels of acute phase proteins from animals with colic, before and after laparotomy, however with higher levels, premature and persistent on animals from G3. The results were due to the process of intestinal inflammation, caused by enteric injury and indicate that the serum protein

  15. PRODUCTION OF DISSOLVING GRADE PULP FROM ALFA

    Directory of Open Access Journals (Sweden)

    Baya Bouiri

    2010-02-01

    Full Text Available Alfa, also known as Stipa tenacissimaI or “halfa”, is grown in North Africa and south Spain. Due to its short fiber length, paper made from alfa pulp retains bulk and takes block letters well. In this study alfa was evaluated for bleached pulp production. Two cellulose pulps with different chemical compositions were pulped by a conventional kraft process. One sample was taken from the original alfa material and another from alfa that had been pretreated by diluted acid. The pulp produced from the pretreated alfa was bleached by the elemental-chlorine-free sequences DEPD and DEDP. The yield, Kappa number, brightness, and α- cellulose content of bleached and unbleached pulps were evaluated. The results showed that during the chemical pulping process, treated alfa cooked more easily than the original alfa. The treated alfa pulp also showed very good bleaching, reaching a brightness level of 94.8% ISO with a yield of 93.6% at an α-cellulose content 96.8(% with a DEDP bleaching sequence, compared to 83.2% ISO brightness level, 92.8% yield, and 95.1% α-cellulose content for bleached pulp with a DEPD bleaching sequence. Therefore, this alfa material could be considered as a worthwhile choice for cellulosic fiber supply.

  16. The ALFA Trigger Simulator

    CERN Document Server

    Dziedzic B

    2015-01-01

    The paper presents basic information about ALFA detectors used in the ATLAS experiment, and the structure of currently developed device used to test a new ALFA trigger interface. It discusses the block diagram of the device, principle of its operation, implementation details and future plans for developing the Simulator.

  17. ALFA detector upgrade before LHC Run 2

    CERN Document Server

    Vorobel, Vit; The ATLAS collaboration

    2016-01-01

    The operation experience with ATLAS ALFA detectors in the LHC environment during the Run1 period has shown significant beam-induced heating. Subsequent comprehensive studies revealed that heating effects could be disastrous in the case of the larger beam intensities foreseen for higher luminosities in the LHC Run2. During the first LHC long shutdown (LS1) all ALFA detectors have been removed from the LHC tunnel and their covers - Roman Pots - underwent a geometry upgrade to minimize the impedance losses. It will be shown that this modification together with a system improving the internal heat transfer and an air cooling system, significantly shifted the temperatures of ALFA detectors away from the critical limits throughout the LHC Run2. Also ALFA trigger system was considerably upgraded to keep measured data safely inside the Run2 ATLAS latency budget and to minimize dead time. The needed hardware changes of the trigger system will be presented in the second part of the talk.

  18. ALFA Detector Control System

    CERN Document Server

    Oleiro Seabra, Luis Filipe; The ATLAS collaboration

    2015-01-01

    ALFA (Absolute Luminosity For ATLAS) is one of the sub-detectors of ATLAS (A Toroidal LHC Apparatus). The ALFA system is composed by four stations installed in the LHC tunnel 240 m away from the ATLAS interaction point. Each station has a vacuum and ventilation system, movement control and all the required electronics for signal processing. The Detector Control System (DCS) provides control and monitoring of several components and ensures the safe operation of the detector contributing to good Data Quality. This paper describes the ALFA DCS system including a detector overview, operation aspects and hardware control through a SCADA system, WinCC OA.

  19. ALFA Detector Control System

    CERN Document Server

    Oleiro Seabra, Luis Filipe; The ATLAS collaboration

    2015-01-01

    ALFA (Absolute Luminosity For ATLAS) is one of the sub-detectors of ATLAS/LHC. The ALFA system is composed by two stations installed in the LHC tunnel 240 m away from each side of the ATLAS interaction point. Each station has a vacuum and ventilation system, movement control and all the required electronic for signal processing. The Detector Control System (DCS) provides control and monitoring of several components and ensures the safe operation of the detector contributing to good Data Quality. This paper describes the ALFA DCS system including a detector overview, operation aspects and hardware control through a SCADA system, WinCC OA.

  20. Interferon Alfa-2b Injection

    Science.gov (United States)

    Interferon alfa-2b injection is used to treat a number of conditions.Interferon alfa-2b injection is used alone or in ... Hodgkin's lymphoma (NHL; a slow-growing blood cancer). Interferon alfa-2b is in a class of medications ...

  1. TARTIŞMAYI SONLANDIRMAK: CRONBACH'IN ALFA KATSAYISI, İKİ DEĞERLİ [0,1] ÖLÇÜMLENMİŞ MADDELER İLE KULLANILABİLİR

    OpenAIRE

    Bademci, Vahit

    2010-01-01

    ÖzetÖlçümlerin güvenirliğini kestirmek için değişik yöntemler vardır. Bunlardan biriside Cronbach'ın alfa katsayısı yöntemidir. Cronbach'ın alfa katsayısı ile ilgili olarak da, bukatsayının sadece ağırlıklı ölçümlenmiş (Örneğin, 1,2,3,4,5 gibi) maddelerlekullanılabileceği şeklinde bir görüş vardır. Ancak, bazı bilim adamlarınca ileri sürülen bugörüş doğru değildir. Çünkü, Cronbach'...

  2. Neuregulinas 1-alfa e 1-beta na regeneração de nervos periféricos Neuregulins 1-alpha and 1-beta on the regeneration the peripheral nerves

    Directory of Open Access Journals (Sweden)

    Fabiano Inácio de Souza

    2010-01-01

    Full Text Available OBJETIVO: Avaliar o efeito das neuregulinas 1-alfa e 1-beta na regeneração de nervos ciáticos de camundongos C57BL/6J, adultos, machos, através da técnica de tubulização. MÉTODOS: Utilizaram-se 18 animais, divididos em 3 grupos, implantando-se prótese de polietileno em falhas de 4,0 mm no nervo ciático esquerdo: grupo 1 contendo apenas colágeno purificado (Vitrogen®; grupo 2, colágeno associado a neuregulina 1-alfa; grupo 3 com colágeno e neuregulina 1-beta. O grupo controle foi formado por 6 segmentos de nervos ciáticos direitos. Após 4 semanas, os animais foram sacrificados; extraiu-se segmento do ponto médio do nervo regenerado no interior das próteses, padronizaram-se cortes histológicos e confecção das lâminas para análise histomorfométrica. Confrontaram-se os resultados estatisticamente. RESULTADOS: Os animais tratados com neuregulinas tiveram maior número de axônios mielinizados, com diferença estatisticamente significante quando comparados ao grupo colágeno. Não houve diferença estatística entre os grupos de neuregulinas 1-alfa e 1-beta. CONCLUSÃO: a adição de neuregulinas proporcionou aumento significativo do número de fibras mielinizadas.OBJECTIVE: to evaluate the effect of the neuregulins 1-alpha and 1-beta on the regeneration the sciatic nerves of male adult C57BL/6J mice, using the tubulization technique. METHODS: eighteen animals were used, divided into three groups. A polyethylene prosthesis was implanted in a 4.0 mm defect of the left sciatic nerve, as follows: group 1 containing only purified collagen (Vitrogen®; group 2, collagen with neuregulin 1-alpha; group 3, collagen with neuregulin 1-beta. The control group consisted of six segments of right sciatic nerves. After four weeks, the animals were sacrificed. A segment from the midpoint of the nerve regenerated inside the prostheses was extracted; histological sections were standardized, and slides were made up for histomorphometric analysis

  3. Conestat alfa for the treatment of angioedema attacks

    Directory of Open Access Journals (Sweden)

    Davis B

    2011-07-01

    Full Text Available Benjamin Davis, Jonathan A BernsteinUniversity of Cincinnati College of Medicine, Department of Internal Medicine, Division of Immunology/Allergy Section, Cincinnati, OH, USAAbstract: Recently, multiple C1 inhibitor (C1-INH replacement products have been approved for the treatment of hereditary angioedema (HAE. This review summarizes HAE and its current treatment modalities and focuses on findings from bench to bedside trials of a new C1-INH replacement, conestat alfa. Conestat alfa is unique among the other C1-INH replacement products because it is produced from transgenic rabbits rather than derived from human plasma donors, which can potentially allow an unlimited source of drug without any concern of infectious transmission. The clinical trial data generated to date indicate that conestat alfa is safe and effective for the treatment of acute HAE attacks.Keywords: androgens, adverse events, patients, HAE attacks 

  4. Peginterferon Alfa-2a Injection

    Science.gov (United States)

    ... IMPORTANT WARNING section or any of the following: anemia (red blood cells do not bring enough oxygen to other parts of the body), or problems with your eyes or pancreas.tell your doctor if you are pregnant, plan to become pregnant or are breastfeeding. Peginterferon alfa-2a may harm the fetus or ...

  5. Comparison of peginterferon alfa-2a and alfa-2b for treatment of patients with chronic hepatitis C:a retrospective study using the Japanese Interferon Database

    Directory of Open Access Journals (Sweden)

    Sato I

    2014-12-01

    Full Text Available Izumi Sato,1 Takuro Shimbo,2 Yohei Kawasaki,3 Naohiko Masaki41Department of Clinical Study and Informatics, Center for Clinical Sciences, National Center for Global Health and Medicine, Tokyo, Japan; 2Ohta Nishinouchi Hospital, Fukushima, Japan; 3Department of Mathematics, Tokyo University of Science, Tokyo, Japan; 4The Research Center for Hepatitis and Immunology, National Center for Global Health and Medicine, Chiba, JapanPurpose: We aimed to compare the rates of sustained virologic response (SVR achieved with peginterferon (PEG-IFN alfa-2a and alfa-2b in combination with ribavinin (RBV for chronic hepatitis C, using a large database of hepatitis cases to improve the generalizability of these results.Methods: We identified patients with chronic hepatitis C who were treated with PEG-IFN alfa-2a or alfa-2b and RBV, from the Japanese Interferon Database, between December 2009 and April 2013. This database contains the medical records of IFN treatment collected from 36 prefectures in Japan. Multivariable logistic regression analysis was used to compare SVR rates obtained with PEG-IFN alfa-2a and alfa-2b, in combination with RBV. Results: A total of 16,349 patients were recorded in the Japanese Interferon Database. After application of the exclusion criteria, 12,706 patients (3,578 [1,710 males, 1,868 females] on PEG-IFN alfa-2a; and 9,128 [4,652 males, 4,476 females] on PEG-IFN alfa-2b were included in this analysis. The SVR rate in the PEG-IFN alfa-2b group was 62.0%, as compared with a rate of 55.1% in the PEG-IFN alfa-2a group (crude odds ratio =1.31; 95% confidence interval [CI]: 1.23 to 1.44. There was no significant difference in the adjusted SVR rates between the two groups (adjusted odds ratio =0.96; 95% CI: 0.88 to 1.05. Similar proportions of adverse events were observed in the two groups, with the exception of thrombocytopenia, retinopathy, and anemia.Conclusion: There was no significant difference in the SVR rates and safety profile

  6. Thalidomide with peginterferon alfa-2b and ribavirin in the treatment of non-responders genotype 1 chronic hepatitis C patients: proof of concept Talidomida, peginterferón alfa-2b y ribavirina en el tratamiento de pacientes no respondedores con hepatitis crónica C genotipo 1: estudio piloto

    Directory of Open Access Journals (Sweden)

    Benjamín Pardo-Yules

    2011-12-01

    Full Text Available Background: fewer than half of patients infected with hepatitis C virus (HCV achieve sustained viral clearance after peginterferon alfa/ribavirin (Peg-IFN/RBV therapy. Aims: thalidomide posses anti-inflammatory and immunomodulatory properties through inhibition of tumor necrosis factor and costimulatory effect on human CD8+ T cells. Methods: we started a prospective, open label trial of retreatment of very-difficult-to-treat genotype 1 chronic hepatitis C patients (CHC patients, who had failed to respond to the (Peg-IFN/RBV, with a triple therapy consisting in these same antivirals plus thalidomide 200 mg/day (the TRITAL study. Results: none of the eleven patients fulfilling the inclusion criteria and included in the trial reached complete early virological response or sustained virological response. Viral load decline after 12 weeks of triple therapy thalidomide-based retreatment did not differ from viral dynamics during the first course. The triple therapy was well tolerated and only one patient developed mild bilateral neuropathy. Conclusions: thalidomide addition to standard therapy is tolerated and did not increase the SVR rate in very-difficult-to-treat genotype 1 CHC patients. Different schedules are warranted to improve attempting retreatment of non responder CHC patients.Antecedentes: menos de la mitad de los pacientes con hepatitis C logra eliminar el virus de manera sostenida después de la terapia con peginterferón alfa y ribavirina (Peg-IFN/RBV. Objetivos: la talidomida posee propiedades antiinflamatorias e inmunomoduladoras a través de la inhibición del TNF-α y al efecto estimulador sobre las células T CD8+. Métodos: se inició un estudio prospectivo y abierto de re-tratamiento de pacientes con hepatitis crónica C genotipo 1, no respondedores al tratamiento con Peg-IFN/RBV, mediante triple terapia añadiendo a los mismos antivirales 200 mg/día de talidomida. Resultados: ninguno de los once pacientes que fueron incluidos en

  7. The ALFA ZOA Deep Survey: First Results

    CERN Document Server

    McIntyre, Travis P; Minchin, Robert F; Momjian, Emmanuel; Butcher, Zhon

    2015-01-01

    The Arecibo L-Band Feed Array Zone of Avoidance (ALFA ZOA) Deep Survey is the deepest and most sensitive blind Hi survey undertaken in the ZOA. ALFA ZOA Deep will cover about 300 square degrees of sky behind the Galactic plane in both the inner (30 deg < l < 75 deg; b < |2 deg|) and outer (175 deg < l < 207 deg; -2 deg < b < +1 deg) Galaxy, using the Arecibo Radio Telescope. First results from the survey have found 61 galaxies within a 15 square degree area centered on l = 192 deg and b = -2 deg. The survey reached its expected sensitivity of rms = 1 mJy at 9 km/s channel resolution, and is shown to be complete above integrated flux, F_HI = 0.5 Jy km/s. The positional accuracy of the survey is 28 arcsec and detections are found out to a recessional velocity of nearly 19,000 km/s. The survey confirms the extent of the Orion and Abell 539 clusters behind the plane of the Milky Way and discovers expansive voids, at 10,000 km/s and 18,000 km/s. 26 detections (43%) have a counterpart in the li...

  8. Interacción de leucocitos humanos con la proteína de matriz extracelular fibronectina : regulación y función de la integrina [alfa]4[beta]1

    OpenAIRE

    Sánchez Aparicio, Paloma

    1994-01-01

    Las interacciones celula-celula y celula-matriz extracelular son importantes para la función general de las células. La interacción de los leucocitos con la matriz extracelular (mec) es esencial para la migración, localización en tejidos, desarrollo y función de estas células esta tesis se centra en el estudio de la interaccion de los leucocitos humanos con la proteina de mec fibronectina (fn), asi como en el análisis de uno de sus receptores celulares, la integrina alfa4 beta1. En este conte...

  9. Epoetin alfa in platinum-treated ovarian cancer patients: results of a multinational, multicentre, randomised trial

    Science.gov (United States)

    Wilkinson, P M; Antonopoulos, M; Lahousen, M; Lind, M; Kosmidis, P

    2006-01-01

    This multicentre, open-label, controlled clinical trial assessed the effects of epoetin alfa treatment on haematologic and quality of life (QOL) parameters in 182 anaemic (Hb⩽12 g dl−1) ovarian cancer patients receiving platinum chemotherapy. Patients were randomised 2 : 1 to receive epoetin alfa 10 000–20 000 IU three times weekly plus best standard treatment (BST) or BST only. Main study end points were changes from baseline in haemoglobin (Hb) level, transfusion requirements, and QOL. For the epoetin alfa group, mean Hb increased by 1.8 g dl−1 by weeks 4–6 and was significantly increased from baseline through study end (P<0.001). The mean change in Hb from baseline was significantly (P<0.001) greater for epoetin alfa than BST patients at all postbaseline evaluations. Significantly fewer epoetin alfa than BST patients required transfusion(s) after the first 4 weeks of treatment (7.9 vs 30.5%; P<0.001). Also, significant (P⩽0.04) differences favouring the epoetin alfa group over the BST group were found for all three median CLAS scores (Energy Level, Ability to Do Daily Activities, Overall QOL) and the median average CLAS score during chemotherapy. These findings support use of epoetin alfa to increase Hb levels, reduce transfusion use, and improve QOL in anaemic ovarian cancer patients receiving platinum chemotherapy. PMID:16570051

  10. Peginterferon Alfa-2b (PEG-Intron)

    Science.gov (United States)

    ... damage and who have not been treated with interferon alpha (medication similar to peginterferon alfa-2b) in the ... doctor prescribed. Do not use another brand of interferon or switch between peginterferon alfa-2b in vials and injection pens without talking ...

  11. Elongaatiotekijä alfa yhden sitoutuminen promoottorialueelle

    OpenAIRE

    Eskandari, Kowstan

    2012-01-01

    Tämä opinnäytetyö tehtiin Åbo Akademin biotieteiden laitoksella, professori Lea Sistosen laboratoriossa BioCityssä. Opinnäytetyössä tutkittiin, miten solut reagoivat stressiin ja miten ne selviävät stressistä. Päähuomiona oli lämpöshokkitekijä 1:n (HSF1) ja elongaatiotekijä alfa 1:n (eEFα-1) vuorovaikutus DNA:n kanssa.Opinnäytetyössä käytettiin oligonukleotidipresipitaatio-menetelmää, jolla tietylle emäsjärjestykselle sitoutuneet proteiinit eristetään. Työ sisälsi paljon soluviljelyä, tu...

  12. ALFA: The new ALICE-FAIR software framework

    Science.gov (United States)

    Al-Turany, M.; Buncic, P.; Hristov, P.; Kollegger, T.; Kouzinopoulos, C.; Lebedev, A.; Lindenstruth, V.; Manafov, A.; Richter, M.; Rybalchenko, A.; Vande Vyvre, P.; Winckler, N.

    2015-12-01

    The commonalities between the ALICE and FAIR experiments and their computing requirements led to the development of large parts of a common software framework in an experiment independent way. The FairRoot project has already shown the feasibility of such an approach for the FAIR experiments and extending it beyond FAIR to experiments at other facilities[1, 2]. The ALFA framework is a joint development between ALICE Online- Offline (O2) and FairRoot teams. ALFA is designed as a flexible, elastic system, which balances reliability and ease of development with performance using multi-processing and multithreading. A message- based approach has been adopted; such an approach will support the use of the software on different hardware platforms, including heterogeneous systems. Each process in ALFA assumes limited communication and reliance on other processes. Such a design will add horizontal scaling (multiple processes) to vertical scaling provided by multiple threads to meet computing and throughput demands. ALFA does not dictate any application protocols. Potentially, any content-based processor or any source can change the application protocol. The framework supports different serialization standards for data exchange between different hardware and software languages.

  13. ALFA: Automated Line Fitting Algorithm

    Science.gov (United States)

    Wesson, R.

    2015-12-01

    ALFA fits emission line spectra of arbitrary wavelength coverage and resolution, fully automatically. It uses a catalog of lines which may be present to construct synthetic spectra, the parameters of which are then optimized by means of a genetic algorithm. Uncertainties are estimated using the noise structure of the residuals. An emission line spectrum containing several hundred lines can be fitted in a few seconds using a single processor of a typical contemporary desktop or laptop PC. Data cubes in FITS format can be analysed using multiple processors, and an analysis of tens of thousands of deep spectra obtained with instruments such as MUSE will take a few hours.

  14. DNA segregation by the bacterial actin AlfA during Bacillus subtilis growth and development

    OpenAIRE

    Becker, Eric; Herrera, Nick C; Gunderson, Felizza Q.; Derman, Alan I.; Dance, Amber L; Sims, Jennifer; Larsen, Rachel A.; Pogliano, Joe

    2006-01-01

    We here identify a protein (AlfA; actin like filament) that defines a new family of actins that are only distantly related to MreB and ParM. AlfA is required for segregation of Bacillus subtilis plasmid pBET131 (a mini pLS32-derivative) during growth and sporulation. A 3-kb DNA fragment encoding alfA and a downstream gene (alfB) is necessary and sufficient for plasmid stability. AlfA-GFP assembles dynamic cytoskeletal filaments that rapidly turn over (t1/2

  15. AFP and ALFA detectors as a momentum spectrometer system - feasibility study

    CERN Document Server

    ABDUL KHALEK, Rabah

    2015-01-01

    We consider a possibility to measure a leading proton momentum using simultaneously the AFP and ALFA detectors. The idea is based on the simple fact that the proton trajectory through the magnetic field between the AFP and ALFA$_{1}$ detectors, depends on the proton's momentum loss $\\xi$. The feasibility of the proton momentum measurement is directly related to the availability of the magnetic fields and their configuration, the overlap between ALFA and AFP detectors and their resolutions as well as energy losses and the multiple scattering effect that could wash away little actions from the magnets on the proton.

  16. "Alfa" i "Võmpel" bez maski / Andrei Sharov

    Index Scriptorium Estoniae

    Sharov, Andrei

    2004-01-01

    Venemaa FSB keskuse, mille koosseisu kuuluvad ka eriüksused Alfa ja Võmpel, töötajad osalevad terroristide neutraliseerimise ja pantvangide vabastamise erioperatsioonides. Intervjuu keskuse staabi uue juhiga

  17. Turoctocog alfa: an evidence-based review of its potential in the treatment of hemophilia A

    Directory of Open Access Journals (Sweden)

    Takedani H

    2015-03-01

    Full Text Available Hideyuki Takedani, Jun Hirose Department of Joint Surgery, Research Hospital of the Institute of Medical Science, The University of Tokyo, Tokyo, Japan Abstract: Turoctocog alfa is the first B-domain-truncated third generation recombinant coagulation factor VIII (FVIII product. Nonclinical in vitro and animal model studies have demonstrated that turoctocog alfa has similar functional potency and hemostatic efficacy as comparator FVIII products. With respect to discrepancies in the level of FVIII concentrate in plasma of current FVIII products on comparing measurement results between one-stage clot and chromogenic assays, there was no difference in the in vitro turoctocog alfa study; however, measured FVIII concentrate in field study was higher with the chromogenic assay (1.08 IU/mL than with one-stage assay (0.83 IU/mL. Two published clinical studies on previously treated patients (PTPs and clinical pharmacokinetics have described that the pharmacokinetic parameters are similar, and the safety and efficacy for prevention and treatment for bleeding are also similar to those of standard half-life FVIII products. Three clinical trials are ongoing to assess the long-term safety and efficacy of turoctocog alfa for PTPs and previously untreated patients. Those data will be published in the near future, and it will be possible to use turoctocog alfa for all hemophilia patients. However, studies will be needed to confirm the turoctocog alfa profile, such as the stability of dissolved turoctocog alfa over 24 hours at room temperature and post-marketing clinical research aimed at meeting Europe Medicines Agency post-marketing safety and efficacy requirements in PTPs. It is recommended to wait before using turoctocog alfa for previously untreated patients and major surgery until further data have been collected and published. Keywords: hemophilia A, B-domain-truncated, third generation factor VIII product, previous untreated patients, chromogenic assay

  18. Agalsidase alfa: a review of its use in the management of Fabry disease.

    Science.gov (United States)

    Keating, Gillian M

    2012-10-01

    stage 1 or 2 chronic kidney disease. Certain benefits of agalsidase alfa became apparent with longer-term therapy. For example, a significant reduction in cold and warm detection thresholds and a significant improvement in sweat function were seen after 3 years' therapy. Final results from a head-to-head trial comparing the effects of agalsidase alfa and agalsidase beta at approved dosages are not yet available. The only available fully published study compared agalsidase alfa 0.2 mg/kg every other week with an off-label dosage of agalsidase beta 0.2 mg/kg every other week. This randomized, open-label, 24-month trial in adult men and women with Fabry disease generally found no significant differences in outcome between treatment arms. It should be noted that concerns were subsequently raised by the European Medicines Agency regarding the use of agalsidase beta at dosages other than the approved dosage of 1 mg/kg every other week. Preliminary results from an ongoing, randomized, open-label study suggest no differences in outcome between patients with Fabry disease receiving intravenous agalsidase alfa 0.2 mg/kg every other week and those receiving the approved regimen of agalsidase beta 1 mg/kg every other week. In three switching studies, no safety concerns were raised and disease stability was generally maintained following the switch from agalsidase beta 1 mg/kg every other week to agalsidase alfa 0.2 mg/kg every other week. Agalsidase alfa also demonstrated beneficial effects, including in women and pediatric patients, in noncomparative studies and in the Fabry Outcome Survey. Agalsidase alfa was generally well tolerated in patients with Fabry disease, with infusion reactions (e.g. rigors, pyrexia, flushing) being the most commonly occurring adverse event. IgG antibodies developed in ≈24% of male patients with Fabry disease who received agalsidase alfa. After 12-54 months of treatment, 17% of agalsidase alfa recipients were still IgG antibody positive

  19. Corifollitropin alfa versus recombinant follicle-stimulating hormone: an individual patient data meta-analysis.

    Science.gov (United States)

    Griesinger, Georg; Boostanfar, Robert; Gordon, Keith; Gates, Davis; McCrary Sisk, Christine; Stegmann, Barbara J

    2016-07-01

    A meta-analysis was conducted of individual patient data (n = 3292) from three randomized controlled trials of corifollitropin alfa versus rFSH: Engage (150 µg corifollitropin alfa n = 756; 200 IU rFSH n = 750), Ensure (100 µg corifollitropin alfa n = 268; 150 IU rFSH n = 128), and Pursue (150 µg corifollitropin alfa n = 694; 300 IU rFSH n = 696). Women with regular menstrual cycles aged 18-36 and body weight >60 kg (Engage) or ≤60 kg (Ensure), or women aged 35-42 years and body weight ≥50 kg (Pursue), received a single injection (100 µg or 150 µg) of corifollitropin alfa (based on body weight and age) or daily rFSH. The difference (corifollitropin alfa minus rFSH) in the number of oocytes retrieved was +1.0 (95% CI: 0.5-1.5); vital pregnancy rate: -2.2% (95% CI: -5.3%-0.9%); ongoing pregnancy rate: -1.7% (95% CI: -4.7%-1.4%); and live birth rate: -2.0% (95% CI: -5.0%-1.1%). The odds ratio for overall OHSS was 1.15 (95% CI: 0.82-1.61), and for moderate-to-severe OHSS: 1.29 (95% CI: 0.81-2.05). A single dose of corifollitropin alfa for the first 7 days of ovarian stimulation is a generally well-tolerated and similarly effective treatment compared with daily rFSH. PMID:27178762

  20. Psychiatric side effects of pegylated interferon alfa-2b as compared to conventional interferon alfa-2b in patients with chronic hepatitis C

    Institute of Scientific and Technical Information of China (English)

    Michael R Kraus; Arne Sch(a)fer; Herbert Csef; Michael Scheurlen

    2005-01-01

    AIM: To assess systematically the spectrum and extent of depressive symptoms comparing patient groups receiving peginterferon or conventional interferon.METHODS: Ninety-eight patients with chronic hepatitis C and interferon-based therapy (+ribavirin) were consecutively enrolled in a longitudinal study. Patients were treated with conventional interferon alfa-2b (48/98patients; 5 MIU interferon alfa-2b thrice weekly) or peginterferon alfa-2b (50/98 patients; 80-150 μgpeginterferon alfa-2b) in combination with weight-adapted ribavirin (800-1 200 mg/d). Repeated psychometric testing was performed before, three times during and once after antiviral therapy: Depression was evaluated by the Hospital Anxiety and Depression Scale (HADS), anger/hostility by the Symptom Checklist-90 Items Revised (SCL-90-R).RESULTS: Therapy with pegylated interferon alfa-2bproduces comparable scores for depression (ANOVA:P = 0.875) as compared to conventional interferon.Maximums of depression scores were even higher and cases of clinically relevant depression were frequent during therapy with peginterferon. Scores for anger/hostility were comparable for both therapy subgroups.CONCLUSION: Our findings suggest that the extent and frequency of depressive symptoms in total are not reduced by peginterferon. Monitoring and management of neuropsychiatric toxicity especially depression have to be considered as much as in antiviral therapy with unmodified interferon.

  1. Calibration, testing, commissioning and first data of ALFA at LHC

    CERN Document Server

    Jakobsen, S; The ATLAS collaboration

    2011-01-01

    The ALFA detector system (Absolute Luminosity For ATLAS) aims for measuring the absolute LHC luminosity with high precision by using pp-scattering under small angles. Scintillating fibers tracking detectors are positioned 240 m from LHC interaction point 1 inside Roman Pots at millimeter distance from the LHC beam axis. The detectors consist of layers of 64 scintillating square fibers of 500 µm size in a U-V configuration. The layers are staggered 10 times to improve the spatial resolution to about 30 micrometers. A total of 11680 scintillating fibers are read out on the 8 ALFA detectors through 184 Multi Anode Photomultiplier Tubes, MAPMTs, of type Hamamatsu R7600-00-M64, 64 channels. Each detector has dedicated scintillating trigger tiles read out by 8 mm Hamamatsu Photomultiplier Tubes (R7400P and R9880U-110) via clear fibers. The gain of the MAPMTs differs up to a factor 3 inside a MAPMT and an additional factor 2 between different MAPMTs. For compensation, ALFA has developed a front-end electronic based...

  2. Upgrade of ATLAS-ALFA for Run2

    CERN Document Server

    Jakobsen, Sune; The ATLAS collaboration

    2016-01-01

    Early experience with the operation of the ALFA detectors in the LHC environment has shown significant beam-induced heating. Comprehensive studies on the effects of heating with a spare detector module have furthermore revealed that heating effects could be disastrous in the case of the larger beam intensities foreseen for higher luminosity. A temporary solution was implemented and kept the detectors below the critical temperature in 2012. In LS1 all ALFA stations have been removed from LHC and are undergoing an upgrade of the geometry of Roman Pots to minimize the impedance losses. When the stations were re-installed ~4 m distance was added between them to gain level arm in the angular measurement between the stations. The updated geometry of the Roman Pot, together with a system to improve the internal heat transfer and an air cooling system, is expected to keep the temperatures of ALFA detectors below critical limits throughout the LHC Run2 and first results from the performance in 2016 will be presented. ...

  3. Interferon alfa with or without ribavirin for chronic hepatitis C

    DEFF Research Database (Denmark)

    Kjaergard, L L; Krogsgaard, K; Gluud, C

    2001-01-01

    To assess the efficacy and safety of interferon alfa with or without ribavirin for treatment of chronic hepatitis C.......To assess the efficacy and safety of interferon alfa with or without ribavirin for treatment of chronic hepatitis C....

  4. Interferon-alfa treatment of facial infantile haemangiomas

    DEFF Research Database (Denmark)

    Fledelius, Hans C.; Illum, N.; Jensen, H.;

    2001-01-01

    ophthalmology, haemangiomas of the face, infancy, Interferon alfa, eye at risk, amblyopia, induced refractive change, ultrasound imaging......ophthalmology, haemangiomas of the face, infancy, Interferon alfa, eye at risk, amblyopia, induced refractive change, ultrasound imaging...

  5. ALFA: an automated line fitting algorithm

    CERN Document Server

    Wesson, Roger

    2015-01-01

    I present the Automated Line Fitting Algorithm, ALFA, a new code which can fit emission line spectra of arbitrary wavelength coverage and resolution, fully automatically. In contrast to traditional emission line fitting methods which require the identification of spectral features suspected to be emission lines, ALFA instead uses a list of lines which are expected to be present to construct a synthetic spectrum. The parameters used to construct the synthetic spectrum are optimised by means of a genetic algorithm. Uncertainties are estimated using the noise structure of the residuals. An emission line spectrum containing several hundred lines can be fitted in a few seconds using a single processor of a typical contemporary desktop or laptop PC. I show that the results are in excellent agreement with those measured manually for a number of spectra. Where discrepancies exist, the manually measured fluxes are found to be less accurate than those returned by ALFA. Together with the code NEAT (Wesson et al. 2012), ...

  6. ALFA: an automated line fitting algorithm

    Science.gov (United States)

    Wesson, R.

    2016-03-01

    I present the automated line fitting algorithm, ALFA, a new code which can fit emission line spectra of arbitrary wavelength coverage and resolution, fully automatically. In contrast to traditional emission line fitting methods which require the identification of spectral features suspected to be emission lines, ALFA instead uses a list of lines which are expected to be present to construct a synthetic spectrum. The parameters used to construct the synthetic spectrum are optimized by means of a genetic algorithm. Uncertainties are estimated using the noise structure of the residuals. An emission line spectrum containing several hundred lines can be fitted in a few seconds using a single processor of a typical contemporary desktop or laptop PC. I show that the results are in excellent agreement with those measured manually for a number of spectra. Where discrepancies exist, the manually measured fluxes are found to be less accurate than those returned by ALFA. Together with the code NEAT, ALFA provides a powerful way to rapidly extract physical information from observations, an increasingly vital function in the era of highly multiplexed spectroscopy. The two codes can deliver a reliable and comprehensive analysis of very large data sets in a few hours with little or no user interaction.

  7. Interferon alfa and ribavirin induced hair changes

    International Nuclear Information System (INIS)

    Combination therapy of Interferon alfa and ribavirin in chronic hepatitis C has well documented cutaneous adverse effects. Most interesting of these has been reported on hair physiology. This study was conducted to determine the frequency and pattern of adverse effects involving hair in patients receiving combination of interferon alfa 2a and ribavirin for chronic hepatitis C. The study was conducted in Department of Dermatology, Division of Medicine Shaikh Zayed Hospital. Thirty Eight patients who completed treatment with interferon alfa (3 MIU subcutaneously thrice weekly) and 1200 mg ribavirin daily for 24 weeks were enrolled in this single-center study. The patient's response and examination finding particularly regarding involvement of hair was noted on a Proforma. Thirty Two out of thirty eight (84%) patients noted adverse effects involving hair. The most frequent was diffuse hair loss and occurred in 27 patients (71%). Hypertrichosis of eyelashes (trichomegaly) and eyebrows (synophyrs) was observed in 18 (47%) and 16 (42%) patients respectively. Graying of hair was noted in 4 patients (11%), while discoloration of moustache hair was seen in 2 patients (5%). Epilation at the site of subcutaneous injection was noted in 10 patients (26%). Alopecia areata was reported in 2 patients (5%). It is concluded that adverse effects involving hair are frequent and varied (hair loss to excess hair growth) during combination therapy with Interferon alfa-2a and Ribavirin for chronic hepatitis C. (author)

  8. Home infusion program for Fabry disease: experience with agalsidase alfa in Argentina

    Directory of Open Access Journals (Sweden)

    Isaac Kisinovsky

    2013-02-01

    Full Text Available Fabry disease is an X-linked lysosomal storage disorder caused by inherited deficiency of the enzyme α-galactosidase A. Enzyme replacement treatment using agalsidase alfa significantly reduces pain, improves cardiac function and quality of life, and slows renal deterioration. Nevertheless, it is a life-long treatment which requires regular intravenous infusions and entails a great burden for patients. Our objective was to evaluate retrospectively the safety and tolerability of the home infusion of agalsidase alfa in patients with Fabry disease in Argentina. We evaluated all the patients with Fabry disease who received home infusion with agalsidase alfa 0.2 mg/kg between January 2005 and June 2011. The program included 87 patients; 51 males (mean age: 30 years and 36 females (mean age: 34 years. A total of 5229 infusions (mean: 59 per patient; range: 1-150 were administered. A total of 5 adverse reactions were seen in 5 patients (5.7% of patients and 0.9% of the total number of infusions. All were mild in severity and resolved by reducing the rate of infusion and by using antihistaminics. All these 5 patients were positive for IgG antibodies, but none of them presented IgE antibodies and none suffered an anaphylactic shock. In our group 18 patients were switched from agalsidase beta to agalsidase alfa without complications. Home infusion with agalsidase alfa is safe, well tolerated and is associated to high compliance.

  9. Epoetin alfa in platinum-treated ovarian cancer patients: results of a multinational, multicentre, randomised trial

    OpenAIRE

    Wilkinson, P. M.; Antonopoulos, M; Lahousen, M; Lind, M.; Kosmidis, P

    2006-01-01

    This multicentre, open-label, controlled clinical trial assessed the effects of epoetin alfa treatment on haematologic and quality of life (QOL) parameters in 182 anaemic (Hb⩽12 g dl−1) ovarian cancer patients receiving platinum chemotherapy. Patients were randomised 2 : 1 to receive epoetin alfa 10 000–20 000 IU three times weekly plus best standard treatment (BST) or BST only. Main study end points were changes from baseline in haemoglobin (Hb) level, transfusion requirements, and QOL. For ...

  10. Combination of "low-dose" ribavirin and interferon alfa-2a therapy followed by interferon alfa-2a monotherapy in chronic HCV-infected non-responders and relapsers after interferon alfa-2a monotherapy

    Institute of Scientific and Technical Information of China (English)

    Perdita Wietzke-Braun; Volker Meier; Felix Braun; Giuliano Ramadori

    2001-01-01

    AIM To report on the efficacy, safety and tolerability of interferon alfa-2a combined with a "low dose" of ribavirin for relapsers and non responders to alpha interferon monotherapy.METHODS Thirty-four chronic hepatitis C virus-infected non-responders to interferon alfa2a monotherapy (a course of at least 3 months treatment) and 13 relapsers to interferon alfa 2a monotherapy (a dose of 3 to 6 million units three times per week for at least 20 weeks but not more than 18 months) were treated with the same dose of interferon alfa-2a used before (3 to 6 million units three times per week) and ribavirin (10 mg/ kg daily) for 6 months. In complete responders, interferon alfa-2a was administered for further 6 months at the same dose used before as monotherapy.RESULTS Seven (20.6%) of 34 non-responders stopped the combined therapy due to adverse events, including two patients with histological and clinical Child A cirrhosis. In 17/27 (63%)non-responders, the combined therapy was stopped after three months because of non-response. Ten of the 27 non-responders completed the 1;2-month treatment course. At a mean follow up of 28 months (16- 37 months)after the treatment, 4/10 (15%) previous non-responders still remained complete responders,All 13 previous relapsers completed the 12-month treatment course. At a mean follow up of 22months (9 - 36 months) after treatment, 6/13(46%) the previous relapsers were stillsustained complete responders.CONCLUSION Our treatment schedule of the combined therapy for 6 months of interferon alfa2a with a low dose of ribavirin (10 mg/kg/day)followed by 6 months of interferon alfa-2amonotherapy is able to induce a sustainedcomplete response rate in 15% of non-responders and 46% of relapsers with chronic hepatitis C virus-related liver diseases comparable to those obtained with the standarddoses of ribavirin 1000 - 1200 mg/day.Randomized prospective controlled trials using lower total amounts of ribavirin in combination with interferon should be

  11. Analysis of the trigger system of the ALFA detectors

    CERN Document Server

    Guerrero Llorente, Alonso

    2015-01-01

    During the CERN Summer Student Programme 2015 two different projects were carried out. Both related with the ALFA subdetector in ATLAS and its trigger system. The frst, and main, project aimed to do an study of the trigger effciency of the ALFA detector at $\\sqrt{s}=8 TeV$ and $\\beta^* = 90m$. The results can be found fully explained in the ATLAS note: "Trigger effciency for the ALFA detector at $\\sqrt{s}=8 TeV$ and $\\beta^* = 90m$". A secondary task consisted on systematical tests of new chips for an update of the ALFA trigger system.

  12. A randomized comparison of every-2-week darbepoetin alfa and weekly epoetin alfa for the treatment of chemotherapy-induced anemia in patients with breast, lung, or gynecologic cancer.

    Science.gov (United States)

    Schwartzberg, Lee S; Yee, Lorrin K; Senecal, Frank M; Charu, Veena; Tomita, Dianne; Wallace, Joel; Rossi, Greg

    2004-01-01

    An important clinical question is the relative efficacy of the most common dosages of darbepoetin alfa (Aranesp; Amgen Inc.; Thousand Oaks, CA) 200 microg every 2 weeks (Q2W) and epoetin alfa (Procrit; Ortho Biotech Products, LP; Raritan, NJ) 40,000 U weekly (QW) for the treatment of chemotherapy-induced anemia. We designed three concurrent randomized, open-label, multicenter, identical trials (with the exception of tumor type criteria of breast, gynecologic, or lung cancer) of darbepoetin alfa and epoetin alfa in patients with chemotherapy-induced anemia to validate the Patient Satisfaction Questionnaire for Anemia (PSQ-An) treatment tool and to compare the efficacies and safety profiles of these two agents. In each trial, patients were randomized 1:1 to receive either darbepoetin alfa at a dose of 200 microg Q2W or epoetin alfa at a dose of 40,000 U QW for up to 16 weeks. The PSQ-An was assessed for validity, feasibility, and reliability. Secondary clinical endpoints were analyzed using the primary analysis set. Both individual trial analyses and a protocol-specified combined analysis of data from all three trials were conducted. Overall, 312 patients (157 darbepoetin alfa; 155 epoetin alfa) were randomized and received study drug. Baseline characteristics were similar in both treatment groups in each trial and overall. The PSQ-An was valid, feasible, and reliable. In general, no difference between treatment groups was observed for hemoglobin- and transfusion-based endpoints in each individual trial or in the combined analysis. From exploratory analyses, achievement and maintenance of a hemoglobin target range (11-13 g/dl) were similar in both groups. No differences in safety were observed. With the PSQ-An, formal comparisons of the impact of anemia therapies on patients and caregivers can be made in future prospective studies. Further, darbepoetin alfa (200 microg Q2W) and epoetin alfa (40,000 U QW) appear to achieve comparable clinical and hematologic outcomes

  13. 普通干扰素1b联合利巴韦林治疗非1b型慢性丙型肝炎患者临床观察%Combination of interferon alfa-1b and ribavirin in the treatment of patients with chronic hepatitis C of non-1b

    Institute of Scientific and Technical Information of China (English)

    雷华; 禹蔚琴; 田波; 戴静毅; 王晴晴; 柏保利; 庄林

    2012-01-01

    目的 探讨不同基因型HCV感染者对普通干扰素联合利巴韦林治疗的疗效.方法 应用型特异性探针杂交法检测106例慢性丙型肝炎患者HCV基因型.应用普通干扰素联合利巴韦林治疗,观察病毒学应答情况.结果 在106例患者中,以HCV 3b(45.3%)、1b(20.8%)和3a(18.9%)型为主,6a(9.4%)和2a(5.7%)型次之;在22例1b型感染者,只有9例接受本治疗方案,82例非1b型感染者完成治疗.非1b型患者的RVR和EVR分别为50%和86.6%;在1b型HCV感染者,22.2%(2/9)获得SVR,而非1b型HCV感染者,76.8%(63/82)获得SVR.结论 普通干扰素联合利巴韦林治疗非1b型慢性丙型肝炎患者疗效较好.%Objective To investigate the efficacies of interferon alfa-1b and ribavirin in treatment of patients with chronic hepatitis C of different genotypes. Methods The genotypes of hepatitis C virus in 106 patients with hepatitis C were detected by genotype-specific probes. The patients with hepatitis C were treated with interferon alfa-1b and ribavirin for 48 weeks. Results Out of 106 patients with hepatitis C,the HCV genotype 3b accounted for 45.3%,lb for 20.8%,and 3a for 18.9%,while we still detected some 6a(9.4%)and 2a(5.7%) positive;Nine out of 22 patients with hepatitis C of lb and other 82 with non-1b finished the regimen;the rapid virological response and early virological response in patient with non-1b were 50% and 86.6%,respectively;22.2%(2/9)of lb infected patients got sustained virological response ,while 76.8% (63/82)of non-1b got SVRO Conclusion Interferon alfa-1b and ribavirin regimen can be selected for hepatitis C patients with non-lb infection.

  14. Analysis of Claviceps africana and C. sorghi from India using AFLPs, EF-1alfa gene intron 4, and Beta-tubulin gene intron 3

    Czech Academy of Sciences Publication Activity Database

    Tooley, P. W.; Bandyopadhyay, R.; Carras, M. M.; Pažoutová, Sylvie

    2006-01-01

    Roč. 101, č. 4 (2006), s. 441-451. ISSN 0953-7562 Institutional research plan: CEZ:AV0Z50200510 Keywords : clavicipitaceae * coevolution * ergot Subject RIV: EE - Microbiology, Virology Impact factor: 1.860, year: 2006

  15. Pegylated interferon alfa and ribavirin for children with chronic hepatitis C

    Directory of Open Access Journals (Sweden)

    Irit Rosen

    2013-01-01

    Full Text Available AIM: To study current treatment options for pediatric hepatitis C infection and their associated success rates. METHODS: We retrospectively reviewed charts of thirty children who had been treated with combination therapy of pegylated interferon alfa plus ribavirin for chronic hepatitis C infection. Patients had been treated with ribavirin (15 mg/kg per day and either pegylated interferon alfa 2a (180 mg/m2 once weekly or pegylated interferon alfa 2b (1.5 mg/kg once weekly. Patients’ follow-up included subjective assessment of complaints, physical examination including weight and height, as well as laboratory evaluations for viral load [before treatment, at 12 wk, and 6 mo following treatment completion, as determined by sustained viral response (SVR], complete blood count, liver enzymes, alkaline phosphatase, bilirubin, renal function tests, and thyroid function tests. For patients not achieving a two log decrease in viral load at treatment week 12, treatment was discontinued and the patient was considered a treatment non-responder. RESULTS: Thirty children aged 3-18 years were included in the study. Twenty patients (11 males, 9 females received pegylated interferon alfa 2b and ten patients (6 males, 4 females received pegylated interferon alfa 2a. Twenty-three patients were infected with genotype 1, six patients were infected with genotype 3, and one patient was infected with genotype 2. Twenty patients (67% achieved SVR. Treatment success rates were 90% with pegylated interferon alfa 2a vs 55% with pegylated interferon alfa 2b. Although a trend was noted for improved outcomes in the group receiving pegylated interferon alfa 2a, there were no statistically significant outcome differences between the two treatment groups (P = 0.1. Treatment success was 56.5% for patients infected with genotype 1 virus, compared to 100% for patients infected with other genotypes (P = 0.064. There was no difference in treatment response between males and females

  16. Drotrecogin alfa (activated in severe sepsis: a systematic review and new cost-effectiveness analysis

    Directory of Open Access Journals (Sweden)

    Brophy James M

    2007-06-01

    Full Text Available Abstract Background Activated drotrecogin alfa (human activated protein C, rhAPC, is produced by recombinant DNA technology, and purports to improve clinical outcomes by counteracting the inflammatory and thrombotic consequences of severe sepsis. Controversy exists around the clinical benefits of this drug and an updated economic study that considers this variability is needed. Methods A systematic literature review was performed using Medline, Embase and the International Network of Agencies for Health Technology Assessment (INAHTA databases to determine efficacy, safety and previous economic studies. Our economic model was populated with systematic estimates of these parameters and with population life tables for longer term survival information. Monte Carlo simulations were used to estimate the incremental cost-effectiveness ratios (ICERs and variance for the decision analytic models. Results Two randomized clinical trials (RCTS of drotrecogin alfa in adults with severe sepsis and 8 previous economic studies were identified. Although associated with statistical heterogeneity, a pooled analysis of the RCTs did not show a statistically significant 28-day mortality benefit for drotrecogin alfa compared to placebo either for all patients (RR: 0.93, 95% CI: 0.69, 1.26 or those at highest risk as measured by APACHE II ≥ 25 (RR: 0.90, 95% CI: 0.54, 1.49. Our economic analysis based on the totality of the available clinical evidence suggests that the cost-effectiveness of drotrecogin alfa is uncertain ( Conclusion The evidence supporting the clinical and economic attractiveness of drotrecogin alfa is not conclusive and further research appears to be indicated.

  17. Sequential combination of glucocorticosteroids and alfa interferon versus alfa interferon alone for HBeAg-positive chronic hepatitis B

    DEFF Research Database (Denmark)

    Mellerup, M T; Krogsgaard, K; Mathurin, P;

    2002-01-01

    Chronic hepatitis B has serious effects on morbidity and mortality. Alfa interferon has been shown to increase the rates of HBeAg-clearance as well as seroconversion to anti-HBe, but response rates are unsatisfactory. Glucocorticosteroid pretreatment may increase the response to alfa interferon....

  18. Sequential combination of glucocorticosteroids and alfa interferon versus alfa interferon alone chronic hepatitis B. Protocol for a Cochrane Review

    DEFF Research Database (Denmark)

    Mellerup, M T; Krogsgaard, K; Mathurin, P;

    2000-01-01

    Chronic hepatitis B has serious effects on morbidity and mortality. Alfa interferon has been shown to increase the rates of HBeAg-clearance as well as seroconversion to anti-HBe, but response rates are unsatisfactory. Glucocorticosteroid pretreatment may increase the response to alfa interferon....

  19. Single-Strand Conformation Polymorphism (SSCP) analysis of alfas1-casein, beta-casein and k-casein genes in Charnequeira Portuguese indigenous goat breed

    OpenAIRE

    Bonifácio, C.; Santos, Ingrid; Belo, Carmona; Cravador, A.

    2001-01-01

    The DNA from eighty goats belonging to the indigenous portuguese caprine breed Charnequeira, ecotype from Beira Baixa (Beiroa), was analysed. Single-strand conformation polymorphisms were identified at exon 9 and at exons 10-11 of the as1-casein gene and at exon 4 of the kcasein. The b-casein gene was found monomorphic at exon 7 in this population sample. The establishment of an association of some of these SSCP polymorphisms with milk production and protein and fat content was at...

  20. The pattern of immune cell infiltration in chromoblastomycosis: involvement of macrophage inflammatory protein-1 alpha/CCL3 and fungi persistence Padrão de infiltração de células do sistema imune na cromomicose: envolvimento de MIP-1 alfa da persistência fúngica

    Directory of Open Access Journals (Sweden)

    Vanuza Cristina Sá

    2007-02-01

    Full Text Available Chromoblastomycosis (CR is a subcutaneous chronic mycosis characterized by a granulomatous inflammatory response. However, little is known regarding the pattern of leukocyte subsets in CR and the pathways involved in their recruitment. The objective of this study was to assess the cellular subsets, chemokine, chemokine receptors and enzymes in CR. The inflammatory infiltrate was characterized by immunohistochemistry using antibodies against macrophages (CD68, Langerhans'cells (S100, lymphocytes (CD3, CD4, CD8, CD45RO, CD20 and CD56 and neutrophils (CD15. The expression of MIP-1alpha (Macrophage inflammatory protein-1alpha, chemokine receptors (CXCR3 and CCR1 and enzymes (superoxide dismutase-SOD and nitric oxide synthase-iNOS was also evaluated by the same method. We observed an increase in all populations evaluated when compared with the controls. Numbers of CD15+ and CD56+ were significantly lower than CD3+, CD4+, CD20+ and CD68+ cells. Statistical analysis revealed an association of fungi numbers with CD3, CD45RO and iNOS-positive cells. Furthermore, MIP-1alpha expression was associated with CD45RO, CD68, iNOS and CXCR3. Our results suggest a possible role of MIP-1alpha and fungi persistence in the cell infiltration in CR sites.A cromomicose é micose subcutânea crônica sistêmica caracterizada por resposta inflamatória crônica granulomatosa. No entanto, existem poucos dados a respeito do padrão de subtipos de leucócitos na cromomicose e sobre as vias envolvidas no recrutamento destas células. O objetivo deste trabalho foi avaliar os tipos celulares, bem como a expressão de quimiocinas, receptores de quimiocinas e enzimas em lesões de cromomicose. O infiltrado inflamatório foi caracterizado por meio de técnica imuno-histoquímica utilizando os seguintes marcadores CD68 (macrófagos, S100 (células de Langerhans, CD3, CD4, CD8, CD45RO, CD20 e CD56 (linfócitos e CD15 (neutrófilos. A expressão de MIP-1alfa (Proteína Inflamat

  1. Peginterferon alpha-2a is associated with higher sustained virological response than peginterferon alfa-2b in chronic hepatitis C: systematic review of randomized trials

    DEFF Research Database (Denmark)

    Awad, Tahany; Thorlund, Kristian; Hauser, Goran;

    2010-01-01

    A combination of weekly pegylated interferon (peginterferon) alpha and daily ribavirin represents the standard of care for the treatment of chronic hepatitis C according to current guidelines. It is not established which of the two licensed products (peginterferon alpha-2a or peginterferon alfa-2b...... alfa-2b plus ribavirin. Overall, peginterferon alpha-2a significantly increased the number of patients who achieved a sustained virological response (SVR) versus peginterferon alfa-2b (47% versus 41%; risk ratio 1.11, 95% confidence interval 1.04-1.19; P = 0.004 [eight trials]). Subgroup analyses......-2a is associated with higher SVR than peginterferon alfa-2b. However, the paucity of evidence on adverse events curbs the decision to definitively recommend one peginterferon over the other, because any potential benefit must outweigh the risk of harm....

  2. Autómatas celulares alfa-beta

    OpenAIRE

    Benjamín Luna Benoso; Cornelio Yáñez Márquez

    2012-01-01

    Los autómatas celulares Alfa-Beta constituyen un puente conceptual entre dos áreas de investigación que han sido ajenas hasta el día de hoy: las memorias asociativas Alfa-Beta, por un lado, y los autómatas celulares, por el otro. Con los resultados de este artículo es posible aplicar las herramientas propias de las memorias asociativas Alfa-Beta en el ámbito de los autómatas celulares y, viceversa, se puede aplicar el bagaje teórico de los autómatas celulares en los algoritmos propios de las ...

  3. Fast radio burst discovered in the Arecibo pulsar ALFA survey

    International Nuclear Information System (INIS)

    Recent work has exploited pulsar survey data to identify temporally isolated, millisecond-duration radio bursts with large dispersion measures (DMs). These bursts have been interpreted as arising from a population of extragalactic sources, in which case they would provide unprecedented opportunities for probing the intergalactic medium; they may also be linked to new source classes. Until now, however, all so-called fast radio bursts (FRBs) have been detected with the Parkes radio telescope and its 13-beam receiver, casting some concern about the astrophysical nature of these signals. Here we present FRB 121102, the first FRB discovery from a geographic location other than Parkes. FRB 121102 was found in the Galactic anti-center region in the 1.4 GHz Pulsar Arecibo L-band Feed Array (ALFA) survey with the Arecibo Observatory with a DM = 557.4 ± 2.0 pc cm–3, pulse width of 3.0 ± 0.5 ms, and no evidence of interstellar scattering. The observed delay of the signal arrival time with frequency agrees precisely with the expectation of dispersion through an ionized medium. Despite its low Galactic latitude (b = –0.°2), the burst has three times the maximum Galactic DM expected along this particular line of sight, suggesting an extragalactic origin. A peculiar aspect of the signal is an inverted spectrum; we interpret this as a consequence of being detected in a sidelobe of the ALFA receiver. FRB 121102's brightness, duration, and the inferred event rate are all consistent with the properties of the previously detected Parkes bursts.

  4. Correlation between mechanical and dielectric properties of Alfa/Wool/Polymeric hybrid fibres reinforced polyester composites

    International Nuclear Information System (INIS)

    Dielectric measurements and tensile testing of polyester/natural fibres (Alfa/wool) and thermo binder fibres (Pe/Pet) composites were investigated in order to study the adhesion of the fibres in the polyester matrix. Two composites #1 and #2 having 17:1:2 and 17:2:1 as a relative fraction of alfa/wool and thermo binder (Pe/Pet), respectively, have been characterized in this study. The obtained results revealed that the fibres adhesion in the matrix was better in the composite #1 than in the composite #2. Indeed, the analysis of the interfacial or Maxwell-Wagner-Sillars (MWS) polarization intensity, using the Havriliak–Negami model, has shown a lower intensity and the tensile testing exhibited a higher Young modulus in the composite #1. So the thermo binder fibres improve this adhesion

  5. Leucograma, proteína C reativa, alfa-1 glicoproteína ácida e velocidade de hemossedimentação na apendicite aguda Leucocyte count, C reactive protein, alpha-1 acid glycoprotein and erithrocyte sedimmentation rate in acute appendicitis

    Directory of Open Access Journals (Sweden)

    Bruno Ramalho de Carvalho

    2003-03-01

    Full Text Available RACIONAL: O diagnóstico da apendicite aguda é clínico, mas alguns indivíduos podem apresentar sinais e sintomas pouco característicos. As dificuldades diagnósticas ainda conduzem os cirurgiões à realização de laparotomias desnecessárias, que atingem índices de 15% a 40%. Os exames laboratoriais, assim, podem se tornar complementos úteis no diagnóstico da apendicite aguda. O leucograma parece ser o de maior valor, mas a dosagem das proteínas de fase aguda, em especial da proteína C reativa, é objeto de vários estudos. CASUÍSTICA E MÉTODO: Estudo longitudinal prospectivo, envolvendo 63 pacientes submetidos a apendicectomia por suspeita de apendicite aguda no Hospital das Clínicas da Universidade Federal de Uberlândia, MG, de cujas amostras de sangue foram feitas dosagens das proteínas de fase aguda e leucograma. RESULTADOS: Foram 44 pacientes do sexo masculino e a faixa etária predominante entre 11 e 30 anos. A apendicite aguda foi mais freqüente no sexo masculino (69,8% e na faixa etária entre 11 e 30 anos. O tipo flegmonoso foi mais freqüente (52,4%. O leucograma mostrou-se aumentado em 74,6% dos casos e a elevação da proteína C reativa foi observada em 88,9%. As dosagens de alfa-1 glicoproteína ácida e velocidade de hemossedimentação foram predominantemente normais. A proteína C reativa esteve aumentada em mais de 80% dos casos em todas as idades. O leucograma e a proteína C reativa alteraram-se em 80% dos pacientes com até 24 horas do início dos sintomas. Com tempo de evolução dos sintomas superior a 24 horas, o leucograma mostrou-se alterado em 69,7% dos casos e a proteína C reativa, em 97%. Sensibilidade e especificidade do leucograma foram de 88,7% e 20%. Para a proteína C reativa, foram de 88,9% e 10%, respectivamente. A dosagem da proteína C reativa apresentou maior sensibilidade (96,9% para os casos de evolução com mais de 24 horas, sem qualquer especificidade, entretanto, alfa-1 glicoprote

  6. Role of elosulfase alfa in mucopolysaccharidosis IVA

    Directory of Open Access Journals (Sweden)

    Regier DS

    2016-06-01

    Full Text Available Debra S Regier, Pranoot Tanpaiboon Division of Genetics and Metabolism, Children’s National Medical Center, Washington, DC, USA Abstract: Mucopolysaccharidosis type IVA (MPS IVA or Morquio A is an autosomal recessive lysosomal storage disease which results in a striking skeletal phenotype, but does not negatively impact the intellect of the patient. MPS IVA has a phenotypic continuum that ranges from a severe and rapidly progressing form to a slowly progressive form. The clinical diagnosis is often made in the preschool years based on abnormal bone findings on physical examination and dysplasia on radiographic imaging. Supportive care has been the mainstay in caring for patients. Orthopedic physicians often form the core of the care team due to the early and severe skeletal abnormalities; however, systemic disease is common and requires aggressive monitoring and management. Interdisciplinary care teams often consist of medical geneticists, cardiologists, pulmonary specialists, gastroenterologists, otolaryngologists, audiologists, and ophthalmologists. With the US Food and Drug Administration’s approval of elosulfase alfa, patients >5 years of age now have access to this medication from the time of diagnosis. The clinical trial with once weekly intravenous dosing (2.0 mg/kg per week showed improvement in the 6-minute walk test. The composite end point analysis to evaluate the combining changes from baseline in 6-minute walk test, 3-minute stair climb test, and respiratory function showed that at a dose of 2.0 mg/kg per week, subjects performed better when compared to placebo. This indication was clinically meaningful in the treatment group. The treatment was generally well tolerated, and the uncommon infusion reactions responded well to traditional enzyme replacement therapy infusion reaction management algorithms. Currently, clinical trials are underway to determine the efficacy and safety in MPS IVA patients <5 years of age. Keywords

  7. JSC “ALFA-BANK” marketing policy. problems and perspectives

    OpenAIRE

    Kirillov, A.; Kuznetcova, E.; Martirosian, M.

    2013-01-01

    The article is devoted to the results of JSC “Alfa-Bank” consumers’ segmentation and the following complex marketing research. The article suggests the ways of the bank’s marketing policy improvement.

  8. Treatment of anemia with darbepoetin alfa in systolic heart failure

    DEFF Research Database (Denmark)

    Swedberg, Karl; Young, James B; Anand, Inder S;

    2013-01-01

    Patients with systolic heart failure and anemia have worse symptoms, functional capacity, and outcomes than those without anemia. We evaluated the effects of darbepoetin alfa on clinical outcomes in patients with systolic heart failure and anemia.......Patients with systolic heart failure and anemia have worse symptoms, functional capacity, and outcomes than those without anemia. We evaluated the effects of darbepoetin alfa on clinical outcomes in patients with systolic heart failure and anemia....

  9. Efficacy of Surfactant-TA, Calfactant and Poractant Alfa for Preterm Infants with Respiratory Distress Syndrome: A Retrospective Study

    OpenAIRE

    Jeon, Ga Won; Oh, Minkyung; Sin, Jong Beom

    2015-01-01

    Purpose To compare the efficacy of the new drug calfactant with the commonly used drugs surfactant-TA and poractant alfa. Materials and Methods A total of 332 preterm infants at 24-31 weeks' gestation with respiratory distress syndrome (RDS) were enrolled and allocated to three groups according to the surfactant instilled; Group 1 (n=146, surfactant-TA), Group 2 (n=96, calfactant), and Group 3 (n=90, poractant alfa). The diagnosis of RDS and the decision to replace the pulmonary surfactant we...

  10. Purification and Characterization of Recombinant Darbepoetin Alfa from Leishmania tarentolae.

    Science.gov (United States)

    Kianmehr, Anvarsadat; Mahrooz, Abdolkarim; Oladnabi, Morteza; Safdari, Yaghoub; Ansari, Javad; Veisi, Kamal; Evazalipour, Mehdi; Shahbazmohammadi, Hamid; Omidinia, Eskandar

    2016-09-01

    Darbepoetin alfa is a biopharmaceutical glycoprotein that stimulates erythropoiesis and is used to treat anemia, which associated with renal failure and cancer chemotherapy. We herein describe the structural characterization of recombinant darbepoetin alfa produced by Leishmania tarentolae T7-TR host. The DNA expression cassette was integrated into the L. tarentolae genome through homologous recombination. Transformed clones were selected by antibiotic resistance, diagnostic PCRs, and protein expression analysis. The structure of recombinant darbepoetin alfa was analyzed by isoelectric focusing, ultraviolet-visible spectrum, and circular dichroism (CD) spectroscopy. Expression analysis showed the presence of a protein band at 40 kDa, and its expression level was 51.2 mg/ml of culture medium. Darbepoetin alfa have 5 isoforms with varying degree of sialylation. The UV absorption and CD spectra were analogous to original drug (Aranesp), which confirmed that the produced protein was darbepoetin alfa. Potency test results revealed that the purified protein was biologically active. In brief, the structural and biological characteristics of expressed darbepoetin alfa were very similar to Aranesp which has been normally expressed in CHO. Our data also suggest that produced protein has potential to be developed for clinical use. PMID:27282622

  11. 干扰素α-1b联合恩替卡韦治疗高病毒载量慢性乙型肝炎疗效评价%Efficacy of interferon alfa-1b combined with entecavir in treatment of patients with chronic hepatitis B and high serum viral load

    Institute of Scientific and Technical Information of China (English)

    占国清; 李芳; 李儒贵; 刘翔; 谭华炳

    2016-01-01

    目的:探讨干扰素α-1b联合恩替卡韦(ETV)治疗高病毒载量慢性乙型肝炎(CHB)患者的临床疗效。方法92例高病毒载量(血清HBV DNA≥1×107 IU/ml)CHB患者被分为观察组48例和对照组44例。对照组采用干扰素α-1b治疗,观察组采用干扰素α-1b联合ETV治疗,疗程48 w。比较两组治疗后第12、24、48 w血清丙氨酸氨基转移酶(ALT)复常率、HBV DNA不可检出率、HBeAg阴转率和HBeAg转换率。采用ELISA法检测血清乙型肝炎病毒标记物;使用自动生化分析仪检测肝功能指标;采用荧光定量PCR法检测HBV DNA。结果在治疗第24 w和48 w时,观察组血清ALT复常率分别为68.8%和91.7%,显著高于对照组的34.1%和63.6%(P<0.01);在治疗第12 w、24 w和48 w时,观察组血清HBV DNA不可检测率分别为45.8%、72.9%和87.5%,显著高于对照组的11.4%、31.8%和47.7%(P<0.01);在治疗第48 w时,观察组HBeAg阴转率和HBeAg转换率分别为67.6%和62.2%,显著高于对照组的42.9%和37.1%(P<0.05)。结论干扰素α-1b联合ETV治疗血清高病毒载量的CHB患者疗效优于单用干扰素α-1b。%Objective To investigate the clinical efficacy of interferon alfa-1b combined with entecavir (ETV)in treatment of patients with chronic hepatitis B (CHB) and high serum viral loads. Methods 92 patients with CHB and high serum viral load (HBV DNA≥1×107 IU/ml) were divided into observation group (n=48) and control group (n=44). Patients in the control group were treated with interferon alfa-1b,and patients in the observation group were treated with interferon alfa-1b combined with ETV. The treatment course in two groups lasted for 48 weeks. The recovery rates of serum alanine aminotransferase (ALT),rates of serum HBV DNA loss, negative rates of HBeAg and the conversion rates of HBeAg to anti-HBe were compared at 12,24 and 48 weeks. Serum HBV markers and HBV DNA levels were routinely detected. Results The

  12. ATLAS-ALFA measurements on the total cross section and diffraction

    CERN Document Server

    Mortensen, Simon Stark; The ATLAS collaboration

    2015-01-01

    The measurement of the total pp cross section at the LHC at $\\sqrt{s}=7$ TeV with the ALFA subdetector of ATLAS is presented in this talk. In a special run with $\\beta^*=90$ m beam optics corresponding to an integrated luminosity of 80 $\\text{mb}^{-1}$ the differential elastic cross section is measured in the range from $-t=0.0025\\text{ GeV}^2$ to $-t=0.38\\text{ GeV}^2$. The total cross section $\\sigma(pp\\rightarrow X)$ is extracted using the Optical Theorem by extrapolation of the differential elastic cross section to $t=0\\text{ GeV}^2$. Prospects for diffractive measurements using ALFA to detect the intact proton(s) is also discussed.

  13. Extended dosing of darbepoetin alfa in peritoneal dialysis patients

    Directory of Open Access Journals (Sweden)

    Bridges Ian

    2011-03-01

    Full Text Available Abstract Background Anemia is common among peritoneal dialysis (PD patients, and most patients require erythropoiesis-stimulating agents (ESA to maintain their hemoglobin concentrations within current guideline recommendations. Darbepoetin alfa is an ESA with a 3-fold longer half-life and greater in vivo biological activity than recombinant human erythropoietin, allowing less frequent dosing that may simplify anemia management in these patients, providing benefits to patients, care givers and health care providers. Clinical studies have confirmed the efficacy and safety of darbepoetin alfa administered at extended dosing intervals. However, there are limited data on the management of anemia with ESAs in PD patients in routine clinical practice. The aim of this multicenter observational study in European and Australian dialysis patients was to evaluate darbepoetin alfa administered once every 2 weeks (Q2W in routine clinical practice for 12 months. Methods PD patients ≥18 years old and converting to treatment with darbepoetin alfa Q2W were eligible for enrollment regardless of previous or current ESA use. Patients enrolled in the study were treated according to local usual clinical practice. Data were collected up to 6 months prior to and 12 months after conversion to darbepoetin alfa Q2W. The primary endpoint was hemoglobin concentration 12 months after conversion to darbepoetin alfa Q2W. Results Of the 741 eligible PD patients (mean age, 61 years; male, 57%, 640 (86% completed the study. Mean hemoglobin concentration (g/dL was 11.69 (95% CI, 11.53-11.86 6 months before the conversion, 12.25 (95% CI, 12.13-12.38 at conversion, and 11.88 (95% CI, 11.74-12.02 12 months after conversion to darbepoetin alfa Q2W. The weekly equivalent ESA dose (μg/wk was a geometric mean of 25.24 (95% CI, 23.46-27.15 6 months before conversion, 20.90 (95% CI, 19.13-22.83 immediately before conversion, 18.89 (95% CI, 18.13-19.68 at conversion and 19.04 (95% CI, 17

  14. STUDY OF CYANOETHYLATED ALFA FIBER REINFORCED COMPOSITES BY DYNAMIC MECHANICAL ANALYSIS

    OpenAIRE

    Moufida, Ben; Ridha, Ben

    2008-01-01

    Cyanoethylation of long alfa fibers was studied, and these chemically modified fibers were used to manufacture alfa-polyester composites. The dynamic mechanical thermal properties of an unsaturated polyester resin (cured) and of composites of unmodified and chemically modified alfa-polyester were studied using a dynamic mechanical analyzer over a wide temperature range. The data suggest that in spite of the fragility of the cyanoethylated alfa fibers compared to the unmodified ones, both unmo...

  15. Role of elosulfase alfa in mucopolysaccharidosis IVA.

    Science.gov (United States)

    Regier, Debra S; Tanpaiboon, Pranoot

    2016-01-01

    Mucopolysaccharidosis type IVA (MPS IVA or Morquio A) is an autosomal recessive lysosomal storage disease which results in a striking skeletal phenotype, but does not negatively impact the intellect of the patient. MPS IVA has a phenotypic continuum that ranges from a severe and rapidly progressing form to a slowly progressive form. The clinical diagnosis is often made in the preschool years based on abnormal bone findings on physical examination and dysplasia on radiographic imaging. Supportive care has been the mainstay in caring for patients. Orthopedic physicians often form the core of the care team due to the early and severe skeletal abnormalities; however, systemic disease is common and requires aggressive monitoring and management. Interdisciplinary care teams often consist of medical geneticists, cardiologists, pulmonary specialists, gastroenterologists, otolaryngologists, audiologists, and ophthalmologists. With the US Food and Drug Administration's approval of elosulfase alfa, patients >5 years of age now have access to this medication from the time of diagnosis. The clinical trial with once weekly intravenous dosing (2.0 mg/kg per week) showed improvement in the 6-minute walk test. The composite end point analysis to evaluate the combining changes from baseline in 6-minute walk test, 3-minute stair climb test, and respiratory function showed that at a dose of 2.0 mg/kg per week, subjects performed better when compared to placebo. This indication was clinically meaningful in the treatment group. The treatment was generally well tolerated, and the uncommon infusion reactions responded well to traditional enzyme replacement therapy infusion reaction management algorithms. Currently, clinical trials are underway to determine the efficacy and safety in MPS IVA patients <5 years of age. PMID:27366102

  16. Fast Radio Burst Discovered in the Arecibo Pulsar ALFA Survey

    CERN Document Server

    Spitler, L G; Hessels, J W T; Lorimer, D R; McLaughlin, M A; Chatterjee, S; Crawford, F; Deneva, J S; Kaspi, V M; Wharton, R S; Allen, B; Bogdanov, S; Brazier, A; Camilo, F; Freire, P C C; Jenet, F A; Karako-Argaman, C; Knispel, B; Lazarus, P; Lee, K J; van Leeuwen, J; Lynch, R; Lyne, A G; Ransom, S M; Scholz, P; Siemens, X; Stairs, I H; Stovall, K; Swiggum, J K; Venkataraman, A; Zhu, W W; Aulbert, C; Fehrmann, H

    2014-01-01

    Recent work has exploited pulsar survey data to identify temporally isolated, millisecond-duration radio bursts with large dispersion measures (DMs). These bursts have been interpreted as arising from a population of extragalactic sources, in which case they would provide unprecedented opportunities for probing the intergalactic medium; they may also be linked to new source classes. Until now, however, all so-called fast radio bursts (FRBs) have been detected with the Parkes radio telescope and its 13-beam receiver, casting some concern about the astrophysical nature of these signals. Here we present FRB 121102, the first FRB discovery from a geographic location other than Parkes. FRB 121102 was found in the Galactic anti-center region in the 1.4-GHz Pulsar ALFA survey with the Arecibo Observatory with a DM = 557.4 $\\pm$ 3 pc cm$^{-3}$, pulse width of $3\\; \\pm 0.5$ ms, and no evidence of interstellar scattering. The observed delay of the signal arrival time with frequency agrees precisely with the expectation...

  17. Resolution and t-spectrum for the ALFA detector

    CERN Document Server

    Guergouri, Hichem

    2016-01-01

    My report as Summer Student is in two parts. In the first part I report about my attendance to the run of the ALFA detector to test different collimation schemas and find a new way to get rid of the background. The second part is about the measurement of the resolution of the ALFA detector and comparing the real data with the Monte-Carlo (MC) simulations. First of all I tried to be more familiar with ROOT, worked on understanding the code used to access to the data in the trees inside the Ntuples, plotting histograms,… This was done using old Ntuples which contain MC data.

  18. Estudios sobre alfa-amilasa en saliva como marcador de estrés en la especie humana y otras especies animales= Studies on salivary alpha-amylase as a maker of stress in human and different animal species

    OpenAIRE

    Fuentes Rubio, María

    2014-01-01

    OBJETIVOS 1. Evaluar el uso de la alfa amilasa salivar como marcador de estrés usando técnicas tradicionales (ensayos enzimáticos) en humana, usando como modelo experimental a estudiantes de veterinaria que tenían que realizar una presentación oral de un trabajo en lengua extranjera. 2. Evaluar el uso de la alfa amilasa salivar como marcador de estrés usando técnicas tradicionales (ensayos enzimáticos) en cerdos. 3. Implementar nuevos enfoques para la medición de la alfa-amilasa e...

  19. Physics prospects with the ALFA and AFP detectors

    CERN Document Server

    Hamal, Petr; The ATLAS collaboration

    2013-01-01

    The ATLAS ALFA and AFP detectors - the experimental challenge of measuring forward protons at the LHC. We will describe the experimental challenge presented by the measurement of the forward protons at the LHC. The ATLAS ALFA and AFP projects involve the deployment of detectors capable of detecting protons that emerge intact from the interaction with very small scattering angles. In the case of the ALFA detector, located at ±240m from the ATLAS interaction point, this measurement has to be performed using special high \\beta* beam optics. The detector chosen for the task is a nearly edgeless scintillating fibre tracker capable of making measurements at very low t – below 10-3 GeV2 – in the Nuclear Coulomb interference region. In order to reach these very small t-values we need to deploy the detector very near to the beam line utilizing Roman Pots. The main purpose of the ALFA detector is to measure the total p-p cross-section, a fundamental quantity in hadronic physics of importance to particle and to cos...

  20. Efficacy and safety of peginterferon alfa-2a and ribavirin treatment of chronic hepatitis C in the Republic of Serbia

    Directory of Open Access Journals (Sweden)

    Božić Milena

    2012-01-01

    Full Text Available Introduction. Hepatitis C virus (HCV infection is one of the main causes of chronic liver disease worldwide. Pegylated interferon alfa-2a or 2b (PEG IFN alfa-2a or 2b and ribavirin (RBV represent a standard treatment of chronic hepatitis C (CHC. Sustained virological response (SVR, defined as continued undetectable HCV RNA 24 weeks after completion of treatment, is universally considered as an indicator of treatment efficacy. Objective. The aim of this study was to determine efficacy and safety of PEG IFN alfa-2a and RBV treatment in patients with CHC in Serbia. Methods. One hundred seventy-six patients with CHC were included in this multicenter trial from 8 reference centers in Serbia. The patients were treated with standard PEG IFN alfa- 2a and RBV protocol. We performed the following virological testing: anti-HCV (ELISA, HCV RNK (quantitative PCR, HCV genotype (type-specific PCR, HBsAg, anti-HBs, anti-HBc and anti-HIV (ELISA. Histological activity and the degree of fibrosis were determined according to the Metavir scoring system. Potential predictors for achieving SVR were evaluated using multivariable logistic regression analysis. Results. Of the treated patients with CHC 65.9% were male, and 60.2% of them aged over 40 years. Of the treated patients 68.2% had infection over 5 years, 63% had HCV RNA >400.000 IU/mL, 76.1% had HCV G1/4, and 60.1% had a mild to moderate liver fibrosis. SVR was achieved in 78.9% of patients (G1/4 79.1%; G2/3 78.1%. The factors that indicated a poorer efficacy of the treatment were age >40 (p<0.05, high basal viremia (p=0.013, and the reduction of PEG IFN alfa-2a and RBV doses, with interruption of therapy (p<0.001. Of the treated patients 45.9% had adverse affects (G1/4 50.8%; G2/3 29.7%. Conclusion. Treatment of CHC with PEG IFN alfa-2a and RBV was efficient in 78.9% of patients. The safety profile of therapy was satisfactory. Longer therapy increases the possibility of the development of adverse affects. No

  1. Mapping Hydrogen in the Galaxy, Galactic Halo, and Local Group with ALFA: The GALFA-H I Survey Starting with TOGS

    Science.gov (United States)

    Gibson, S. J.; Douglas, K. A.; Heiles, C.; Korpela, E. J.; Peek, J. E. G.; Putman, M. E.; Stanimirović, S.

    2008-08-01

    Radio observations of gas in the Milky Way and Local Group are vital for understanding how galaxies function as systems. The unique sensitivity of Arecibo's 305 m dish, coupled with the 7-beam Arecibo L-Band Feed Array (ALFA), provides an unparalleled tool for investigating the full range of interstellar phenomena traced by the H I 21 cm line. The GALFA (Galactic ALFA) H I Survey is mapping the entire Arecibo sky over a velocity range of -700 to +700 km s-1 with 0.2 km s-1 velocity channels and an angular resolution of 3.4'. We present highlights from the TOGS (Turn On GALFA Survey) portion of GALFA-H I, which is covering thousands of square degrees in commensal drift scan observations with the ALFALFA and AGES extragalactic ALFA surveys. This work is supported in part by the National Astronomy and Ionosphere Center, operated by Cornell University under cooperative agreement with the National Science Foundation.

  2. Two different durations of adjuvant therapy with intermediate-dose interferon alfa-2b in patients with high-risk melanoma (Nordic IFN trial): a randomised phase 3 trial

    DEFF Research Database (Denmark)

    Hansson, Johan; Aamdal, Steinar; Bastholt, Lars;

    2011-01-01

    Adjuvant high-dose interferon alfa-2b improves relapse-free survival (RFS) in patients with high-risk melanoma, although benefits in overall survival are uncertain. Because of the toxic effects of high-dose regimens, intermediate doses are being explored. We investigated whether adjuvant therapy...... with intermediate-dose interferon alfa-2b for 1 or 2 years would improve outcomes in patients with stage IIB-IIC or III resected cutaneous melanoma....

  3. Evaluation of the efficacy and safety of three dosing regimens of agalsidase alfa enzyme replacement therapy was underpowered

    Directory of Open Access Journals (Sweden)

    Ortiz A

    2015-11-01

    Full Text Available Alberto Ortiz,1,2 Maria Dolores Sanchez-Niño1,2 1Department of Nephrology, IIS-Fundacion Jimenez Diaz, School of Medicine, Universidad Autónoma de Madrid, 2Department of Nephrology, Instituto Reina Sofia de Investigación Nefrológica, Madrid, Spain We read with interest the report by Goláň et al on the “Evaluation of the efficacy and safety of three dosing regimens of agalsidase alfa enzyme replacement therapy in adults with Fabry disease”.1 Based on the reported results, the authors conclude that no efficacy or safety differences were found when the approved every-other-week (EOW dosage of agalsidase alfa was increased to weekly administration. However, the key question is whether the study, as designed and performed, could have had a different outcome. View original article by Goláň et al.

  4. Effect of acupuncture on TNF-alpha, IL-1b and IL-10 concentrations in the peritoneal exudates of carrageenan-induced peritonitis in rats Efeito da acupuntura sobre a concentração de TNF-alfa, IL-1b e IL-10 no exsudato peritoneal de ratos com peritonite induzida por carragenina

    Directory of Open Access Journals (Sweden)

    Márcia Valéria Rizzo Scognamillo-Szabó

    2005-02-01

    Full Text Available Acupuncture is an ancient and empirical therapeutic procedure known by its efficacy in the treatment of pain. However, the influence of acupuncture on inflammatory process is still poorly understood and additional research is needed. In this work, we investigated the mechanism of action of manual acupuncture on the inhibition of neutrophil migration to the peritoneal cavity induced by the inflammatory stimulus carrageenan in Wistar rats. Previous results from our laboratory showed that this anti-inflammatory effect is not due to endogenous corticoid release. Furthermore, the concentration of IL-1b, but not of TNF-alpha or IL-10 in the carrageenan-induced exudates was reduced in the acupuncture group. Further research will be needed to elucidate the mechanisms involved in the anti-inflammatory action of acupuncture as described here.A acupuntura é método terapêutico milenar reconhecido por sua eficácia no tratamento da dor, porém seu efeito sobre processos inflamatórios é ainda pouco conhecido e maiores estudos são necessários. Neste trabalho, é investigado o mecanismo de ação da acupuntura manual sobre a inibição na migração de neutrófilos para a cavidade peritoneal induzida por carragenina em ratos Wistar. Resultados prévios indicam que esse efeito antiinflamatório não depende de hormônios corticóides. Entretanto, as concentrações de IL-1b no exsudato induzido por carragenina foram reduzidas pelo tratamento com acupuntura. Por outro lado os níveis de TNF-alfa e IL-10 não foram afetados pelo tratamento. Mais pesquisas poderão elucidar os mecanismos envolvidos na ação antiinflamatória da acupuntura.

  5. Nonacog alfa: an analysis of safety data from six prospective clinical studies in different patient populations with haemophilia B treated with different therapeutic modalities.

    Science.gov (United States)

    Rendo, Pablo; Smith, Lynne; Lee, Hsiao-Yu; Shafer, Frank

    2015-12-01

    Nonacog alfa is a recombinant factor IX (FIX) product indicated for treatment and prevention of bleeding episodes in patients with haemophilia B. This posthoc analysis evaluated the safety of nonacog alfa in key clinical studies across 15 years. Data were pooled from six prospective studies that utilized on-demand, prophylactic and preventive nonacog alfa regimens: three open-label, nonrandomized studies that assessed efficacy and safety; a bioequivalence study of original and reformulated nonacog alfa; an open-label, randomized study that compared on-demand and prophylactic treatment; and a noninterventional observational registry study that evaluated safety. Safety assessments included adverse events, serious adverse events (SAEs) and events of special interest. In total, 412 patients received nonacog alfa treatment. Adverse events occurred in 220 patients (53.4%), the most common being pyrexia (n = 63), nasopharyngitis (n = 53) and cough (n = 52). Forty-eight patients (11.7%) experienced treatment-related adverse events; the most common were hypersensitivity (n = 6), urticaria (n = 6), FIX inhibition (n = 5) and pyrexia (n = 4). Seventy-four patients (18.0%) developed SAEs. Thirty-seven events of special interest occurred in 31 (7.5%) patients. Events of special interest included allergic-type manifestations (n = 15), inhibitor development (n = 5), lack of effect (n = 8), red blood cell agglutination in tubing or syringe (n = 7), and thrombogenicity (n = 2). Six patients (1.5%) withdrew due to seven adverse events: hypersensitivity (n = 3), drug eruption, pruritic rash, urticaria and decreased therapeutic response (n = 1 each). Four patients died during the study; no deaths were related to study medication. This pooled safety analysis in haemophilia B patients confirmed the safety of nonacog alfa across various patient populations. PMID:26196195

  6. Evidencia de la eficacia de los fármacos anti-TNF Alfa en el tratamiento de la espondilitis anquilosante

    OpenAIRE

    Montilla Salas, Juan Manuel

    2006-01-01

    OBJETIVO Establecer la evidencia de los estudios clínicos sobre la eficacia de las terapias anti-TNF alfa para el tratamiento de la espondilitis anquilosante (EA). MÉTODOS Se realizó una búsqueda con los diferentes fármacos anti-NTF alfa actualmente utilizados en terapia de la espondilitis anquilosante (Infiximab, Etanercept y Adalimumab). La revisión bibliográfica se realizó en Medline, Embase y la librería Cochrane para los estudios controlados y randomizados publicados hasta 1-enero-2005. ...

  7. Calibration, testing, commissioning and first data of ALFA at LHC

    International Nuclear Information System (INIS)

    The ALFA (Absolute Luminosity For ATLAS) project aims at measuring luminosity of the LHC with high precision using pp elastic scattering under small angles. This will be done by moving an upper and a lower tracking detector inside Roman Pots very close to the beam. To align the upper and lower tracking detector a dedicated part overlaps and the alignment will be done with particles passing both the upper and the lower detector. The tracking detectors are made of scintillating square fibers read out by MultiAnode PhotoMutiplier Tubes, MAPMTs, with custom made electronics. The gain of the MAPMT channels has been equalized and the average light yield of scintillating fibers determined before installation in the LHC. Also reflections on the Roman Pot windows have been observed and the window has been blackened to reduce the effect. The ALFA detectors are currently being commissioned at ATLAS/LHC and the first particles have been observed in the detectors.

  8. Calibration, testing, commissioning and first data of ALFA at LHC

    CERN Document Server

    Jakobsen, S; The ATLAS collaboration

    2011-01-01

    The ALFA (Absolute Luminosity For ATLAS) project aims at measuring luminosity of the LHC with high precision by using pp-scattering under small angles. This will be done by moving an upper and a lower tracking detector inside Roman Pots very close to the beam. To align the upper and lower tracking detector a dedicated part overlaps and the alignment will be done with particles passing both the upper and the lower detector. The tracking detectors are made of scintillating square fibers read out by MultiAnode PhotoMutiplier Tubes, MAPMTs, with custom made electronics. The gain of the MAPMT channels has been equalized and the average light yield of scintillating fibers determined before installation in LHC. Also reflections on the Roman Pot windows have been observed and the window has been blackened to reduce the effect. The ALFA detectors are currently being commissioned at ATLAS/LHC and the first particles observed in the detectors.

  9. Pericarditis and chronic inflammatory demyelinating polyneuropathy during therapy with pegylated interferon alfa-2a for chronic hepatitis C

    OpenAIRE

    Nishio, Kazuaki; Konndo, Takeshi; Okada, Shunichi; Enchi, Machiko

    2010-01-01

    We report a case of pericarditis and chronic inflammatory demyelinating polyneuropathy with biological signs of a lupus-like syndrome due to pegylated interferon alfa-2a therapy during treatment for chronic hepatitis C. The patient developed moderate weakness in the lower limbs and dyspnea. He was hospitalized for congestive heart failure. An electrocardiogram showed gradual ST-segment elevation in leads V1 through V6 without coronary artery disease. A transthoracic cardiac ultrasonographic s...

  10. Enhanced antitumor reactivity of tumor-sensitized T cells by interferon alfa

    Energy Technology Data Exchange (ETDEWEB)

    Vander Woude, D.L.; Wagner, P.D.; Shu, S.; Chang, A.E. (Univ. of Michigan, Ann Arbor (USA))

    1991-03-01

    Tumor-draining lymph node cells from mice bearing the methylcholanthrene-induced MCA 106 tumors can be sensitized in vitro to acquire antitumor reactivity. We examined the effect of interferon alfa on the function of cells that underwent in vitro sensitization in adoptive immunotherapy. Interferon alfa increased the antitumor reactivity of in vitro sensitized cells in the treatment of MCA 106 pulmonary metastases. This effect was evident in irradiated mice, indicating that a host response to the interferon alfa was not required. Interferon alfa treatment increased class I major histocompatibility complex antigen expression on tumor cells and increased their susceptibility to lysis by in vitro sensitized cells. These results suggest that interferon alfa enhancement of adoptive immunotherapy was mediated by its effect on tumor cells. Interferon alfa may be a useful adjunct to the adoptive immunotherapy of human cancer.

  11. Drotrecogin alfa (aktiviert) bei der Behandlung der schweren Sepsis

    OpenAIRE

    Schumacher, HK; Müller-Nordhorn, J.; Roll, S.; Willich, SN; Greiner, W

    2007-01-01

    Introduction Sepsis is defined as an invasion of microorganisms and/or their toxins into the blood associated the reaction of the organism to this invasion. Severe sepsis is a major cost driver in intensive care medicine. In Germany, prevalence data was assessed in the context of the German Prevalence Study. Severe sepsis has a prevalence of 35% in German intensive care units. Research questions The following questions were analysed: is Drotrecogin alfa (activated) (DAA) eff...

  12. Pathophysiology of hypophosphatasia and the potential role of asfotase alfa

    Directory of Open Access Journals (Sweden)

    Orimo H

    2016-05-01

    Full Text Available Hideo Orimo Division of Metabolism and Nutrition, Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan Abstract: Hypophosphatasia (HPP is an inherited systemic bone disease that is characterized by bone hypomineralization. HPP is classified into six forms according to the age of onset and severity as perinatal (lethal, perinatal benign, infantile, childhood, adult, and odontohypophosphatasia. The causative gene of the disease is the ALPL gene that encodes tissue-nonspecific alkaline phosphatase (TNAP. TNAP is expressed ubiquitously, and its physiological role is apparent in bone mineralization. A defect in bone mineralization can manifest in several ways, including rickets or osteomalacia in HPP patients. Patients with severe forms suffer from respiratory failure because of hypoplastic chest, which is the main cause of death. They sometimes present with seizures due to a defect in vitamin B6 metabolism resulting from the lack of alkaline phosphatase activity in neuronal cells, which is also lethal. Patients with a mild form of the disease exhibit rickets or osteomalacia and a functional defect of exercise. Odontohypophosphatasia shows only dental manifestations. To date, 302 mutations in the ALPL gene have been reported, mainly single-nucleotide substitutions, and the relationships between phenotype and genotype have been partially elucidated. An established treatment for HPP was not available until the recent development of enzyme replacement therapy. The first successful enzyme replacement therapy in model mice using a modified human TNAP protein (asfotase alfa was reported in 2008, and subsequently success in patients with severe form of the disease was reported in 2012. In 2015, asfotase alfa was approved in Japan in July, followed by in the EU and Canada in August, and then by the US Food and Drug Administration in the USA in October. It is expected that therapy with asfotase alfa will drastically change

  13. PSPP. Análisis de Fiabilidad. Alfa de Cronbach

    OpenAIRE

    Sancho-Álvarez, Carlos; Bakieva, Margarita; González Such, José

    2014-01-01

    Material elaborado en el marco de la convocatoria de Ayudas para el desarrollo de la Innovación Educativa y Calidad Docente del Vicerectorat de Cultura i Igualtat de la Unviversitat de València de 28 de marzo de 2013 Conocer el procedimiento de análisis de fiabilidad en PSPP, centrándonos en el coeficiente Alfa de Cronbach (1951).

  14. Physics prospects with the ALFA and AFP detectors

    CERN Document Server

    Hamal, P; The ATLAS collaboration

    2013-01-01

    We will describe the experimental challenge presented by the measurement of the forward protons at the LHC. The ATLAS ALFA and AFP projects involve the deployment of detectors capable of detecting protons that emerge intact from the interaction with very small scattering angles. In the case of the ALFA detector, located at $\\pm${240}~m from the ATLAS interaction point, this measurement has to be performed using special high $\\beta^*$ beam optics. The detector chosen for the task is a nearly edgeless scintillating fibre tracker capable of making measurements at very low $t$ - below $10^{-3} \\text{GeV}^2$ - in the Nuclear Coulomb interference region. In order to reach these very small $t$ values we need to deploy the detector very near to the beam line utilizing Roman Pots. The main purpose of the ALFA detector is to measure the total p-p cross-section, a fundamental quantity in hadronic physics of importance to particle and to cosmic ray physicists and to provide absolute measurement of luminosity which includ...

  15. Efficasy of interferon Alfa-1b combined with lamivndine in the treatment of chronic hepatitis B%干扰素α-1b联合拉米夫定治疗慢性乙型肝炎

    Institute of Scientific and Technical Information of China (English)

    王冬梅; 楚瑞雪; 王新江

    2005-01-01

    目的探讨干扰素α-1b联合拉米夫定治疗慢性乙型肝炎患者的临床疗效.方法将103例CHB分为三组:干扰素组应用干扰素α-1b 6个月,随访6个月;拉米夫定组应用疗程12个月;联合治疗组为干扰素α-1b联合拉米夫定.采用电化学发光法、荧光定量聚合酶链反应及多点基因正链检测技术对血清ALT、HBV-DNA、HBV-M等指标进行了观察.结果联合治疗组HBV-DNA阴转率高于干扰素组(均P<0.01);HBeAg阴转率和抗-HBe阳转率均高于拉米夫定组(均P<0.01);ALT复常率优于干扰素组和拉米夫定组(P<0.01).结论 IFNα-1b联合拉米夫定治疗慢性乙型肝炎的疗效优于单一用药.

  16. Darbepoetin alfa once every 2 weeks for treatment of anemia in dialysis patients

    DEFF Research Database (Denmark)

    Mann, J; Kessler, M; Villa, Giulio Palludan;

    2007-01-01

    in a large cohort of dialysis patients. METHODS: Data were combined from eight similarly designed 24-week phase 3b European studies, in which patients receiving EPO alfa or beta once-weekly were converted to Q2W darbepoetin alfa. Darbepoetin alfa dosage was titrated to maintain hemoglobin (Hb) between 10......-tolerated. CONCLUSIONS: Q2W darbepoetin alfa is effective in maintaining Hb levels in dialysis patients switched from weekly rHuEPO, regardless of the route of administration and with no notable increase in the weekly equivalent dose....

  17. Immunotherapy with imiquimod and interferon alfa for metastasized Merkel cell carcinoma.

    Science.gov (United States)

    Wahl, R U; Braunschweig, T; Ghassemi, A; Rübben, A

    2016-04-01

    Merkel cell carcinoma (mcc) is a highly aggressive neuroendocrine tumour of the skin. Remission rates are high with chemotherapy in patients with metastasis, but without any improvement in overall survival. We present the case of a 90-year-old woman with facial mcc. After radiation and surgery, the mcc recurred with widespread cutaneous and regional lymph node metastases. The metastases were treated with weekly intralesional injections of 1-2×10(6) IU interferon alfa-2a, accompanied by topical imiquimod 5% cream 3 times weekly. After partial regression, subcutaneous pegylated interferon alfa-2b was added at a dose of 30 μg weekly, which was then increased to 50 μg weekly. At 4 months after the start of immunotherapy, all cutaneous metastases and the intralesionally treated lymph node metastases receded. Interruption or reduction of systemic interferon application resulted in locoregional relapses that were successfully treated with surgery or intralesional interferon injections. The patient remains alive 30 months after initiation of immunotherapy, suggesting that locally metastasized mcc might be able to be controlled with local and systemic immunotherapy. PMID:27122984

  18. Pathophysiology of hypophosphatasia and the potential role of asfotase alfa.

    Science.gov (United States)

    Orimo, Hideo

    2016-01-01

    Hypophosphatasia (HPP) is an inherited systemic bone disease that is characterized by bone hypomineralization. HPP is classified into six forms according to the age of onset and severity as perinatal (lethal), perinatal benign, infantile, childhood, adult, and odontohypophosphatasia. The causative gene of the disease is the ALPL gene that encodes tissue-nonspecific alkaline phosphatase (TNAP). TNAP is expressed ubiquitously, and its physiological role is apparent in bone mineralization. A defect in bone mineralization can manifest in several ways, including rickets or osteomalacia in HPP patients. Patients with severe forms suffer from respiratory failure because of hypoplastic chest, which is the main cause of death. They sometimes present with seizures due to a defect in vitamin B6 metabolism resulting from the lack of alkaline phosphatase activity in neuronal cells, which is also lethal. Patients with a mild form of the disease exhibit rickets or osteomalacia and a functional defect of exercise. Odontohypophosphatasia shows only dental manifestations. To date, 302 mutations in the ALPL gene have been reported, mainly single-nucleotide substitutions, and the relationships between phenotype and genotype have been partially elucidated. An established treatment for HPP was not available until the recent development of enzyme replacement therapy. The first successful enzyme replacement therapy in model mice using a modified human TNAP protein (asfotase alfa) was reported in 2008, and subsequently success in patients with severe form of the disease was reported in 2012. In 2015, asfotase alfa was approved in Japan in July, followed by in the EU and Canada in August, and then by the US Food and Drug Administration in the USA in October. It is expected that therapy with asfotase alfa will drastically change treatments and prognosis of HPP. PMID:27274262

  19. Pegylated interferon alfa-2b plus ribavirin for treatment of chronic hepatitis C

    Institute of Scientific and Technical Information of China (English)

    PN; Rao; Abraham; Koshy; Jacob; Philip; Narayanan; Premaletha; Joy; Varghese; Krishnasamy; Narayanasamy; Samir; Mohindra; Nitin; Vikas; Pai; Manoj; Kumar; Agarwal; Ashokna; Konar; Hasmukh; B; Vora

    2014-01-01

    AIM: To study the safety and efficacy of pegylated interferon alfa-2b, indigenously developed in India, plus ribavirin in treatment of hepatitis C virus(HCV). METHODS: One-hundred HCV patients were enrolled in an open-label, multicenter trial. Patients were treated with pegylated interferon alfa-2b 1.5 μg/kg per week subcutaneously plus oral ribavirin 800 mg/d for patients with genotypes 2 and 3 for 24 wk. The same dose of peginterferon plus weight-based ribavirin(800 mg/d for ≤ 65 kg; 1000 mg/d for > 65-85 kg; 1200 mg/d for > 85-105 kg; 1400 mg/d for > 105 kg body weight) was administered for 48 wk for patients with genotypes 1 and 4. Serological and biochemical responses of patients were assessed.RESULTS: Eighty-two patients(35 in genotypes 1 and 4 and 47 in 2 and 3), completed the study. In genotype 1, 25.9% of patients achieved rapid virologic response(RVR): while the figures were 74.1% for early virologic response(EVR) and 44.4% for sustained virologic response(SVR). For genotypes 2 and 3, all patients bar one belonged to genotype 3, and of those, 71.4%, 87.5%, and 64.3% achieved RVR, EVR, and SVR, respectively. In genotype 4, 58.8%, 88.2%, and 52.9% of patients achieved RVR, EVR, and SVR, respectively. The majority of patients attained normal levels of alanine aminotransferase by 4-12 wk of therapy. Most patients showed a good tolerance for the treatment, although mild-to-moderate adverse events were exhibited; only two patients discontinued the study medication due to serious adverse events(SAEs). Eleven SAEs were observed in nine patients; however, only four SAEs were related to study medication.CONCLUSION: Peginterferon alfa-2b, which was developed in India, in combination with ribavirin, is a safe and effective drug in the treatment of HCV.

  20. A trial of darbepoetin alfa in type 2 diabetes and chronic kidney disease

    DEFF Research Database (Denmark)

    Pfeffer, Marc A; Burdmann, Emmanuel A; Chen, Chao-Yin;

    2009-01-01

    assigned to darbepoetin alfa and 496 patients assigned to placebo (P<0.001). There was only a modest improvement in patient-reported fatigue in the darbepoetin alfa group as compared with the placebo group. CONCLUSIONS: The use of darbepoetin alfa in patients with diabetes, chronic kidney disease, and...... moderate anemia who were not undergoing dialysis did not reduce the risk of either of the two primary composite outcomes (either death or a cardiovascular event or death or a renal event) and was associated with an increased risk of stroke. For many persons involved in clinical decision making, this risk...... tested. METHODS: In this study involving 4038 patients with diabetes, chronic kidney disease, and anemia, we randomly assigned 2012 patients to darbepoetin alfa to achieve a hemoglobin level of approximately 13 g per deciliter and 2026 patients to placebo, with rescue darbepoetin alfa when the hemoglobin...

  1. Assessment of the Results of the Multicenter Randomized Acute Leukemia French Association (ALFA) 9803 Trial%法国急性白血病协会(ALFA)9803多中心随机临床试验结果的评价

    Institute of Scientific and Technical Information of China (English)

    江滨; 凌伟

    2009-01-01

    @@ 1文献来源 Gardin C, Turlure P, Fagot T, et al. Postremission treatment of elderly patients with acute myeloid leukemia in first complete remission after intensive induction chemotherapy: Results of the multicenter randomized Acute Leukemia French Association (ALFA) 9803 trial [J]. Blood, 2007, 109(12) : 5129-5135.

  2. Therapeutic efficacy of a biosimilar epoetin alfa in hemodialysis patients

    Directory of Open Access Journals (Sweden)

    Amel Harzallah

    2015-01-01

    Full Text Available Anemia is a frequent complication in patients with chronic kidney disease. However, human recombinant erythropoietin (rHu-EPO has revolutionized the management of anemia in chronically dialyzed patients. Epomax ® is a new rHu-EPO alfa manufactured in Tunisia (Medis Laboratories. The aim of this study was to evaluate the efficacy and tolerance of Epomax ® in chronic hemodialysis (HD patients in a phase-III, multicenter, clinical trial. Fiftythree HD patients (mean age 47.7 ± 13 years who received a stable dose of rHu-EPO (Hemax ® , a rHu-EPO alfa manufactured by Biosidus Laboratories subcutaneously were switched to Epomax ® via the same route of administration. At baseline, the mean systolic pressure was 132 ± 18 mm Hg and the mean diastolic pressure was 79 ± 8 mm Hg. The mean blood hemoglobin was 10.2 g/dL and the median ferritin level was 667 ng/mL. After a follow-up of 43 days, the mean blood hemoglobin was 10.5 g/dL under the effect of Epomax ® . There was no significant difference in the mean hemoglobin levels between the treatments with both drugs. Few adverse events were reported during the study. We conclude that Epomax ® was effective at maintaining the hemoglobin levels at target concentrations and was well tolerated in HD patients.

  3. Efektivitas Penambahan Vitamin E (alfa-Tokoferol) dalam Medium Pencucian Sperma dengan Sentrifugasi terhadap Kualitas Spermatozoa Sapi Brahman

    OpenAIRE

    Dasrul Dasrul; Rasmaidar Rasmaidar; Abdul Harris

    2012-01-01

    Effect of vitamin E addition (alfa-tokoferol) into sperm washing medium by centrifuge on the quality of Brahman cattle spermatozoa ABSTRACT. The aims of study to determine the effectiveness of the addition of vitamin E in the washing medium by centrifugation on sperm quality Brahman cattle. frozen semen of Brahman cattle, divided into 4 treatment groups addition of vitamin E in the washing medium: 0.0gr/100 ml medium (K0), 0.1gr /100 ml medium (K1); 0.2gr/100 ml medium (K2) and 0.3 g / 10...

  4. Evaluation of pharmacokinetics, user handling, and tolerability of peginterferon alfa-2a (40 kDa delivered via a disposable autoinjector device

    Directory of Open Access Journals (Sweden)

    Varunok P

    2011-11-01

    Full Text Available Peter Varunok1, Eric Lawitz2, Kimberly L Beavers3, Gary Matusow4, Ruby Leong5, Nathalie Lambert6, Coen Bernaards7, Jonathan Solsky5, Barbara J Brennan5, Cynthia Wat8, Anne Bertasso51Gastroenterology Associates, Poughkeepsie, NY, USA; 2Alamo Medical Research, San Antonio, TX, USA; 3Asheville Gastroenterology, Asheville, NC, USA; 4Gastroenterology Group, South Jersey, NJ, USA; 5Roche, Nutley, NJ, USA; 6Roche, Basel, Switzerland; 7Roche, San Francisco, CA, USA; 8Roche, Welwyn, UKBackground: Peginterferon alfa-2a (40 kDa is currently administered using a prefilled syringe. The peginterferon alfa-2a disposable autoinjector is a new safety-engineered device designed to facilitate injection and reduce the risk of needlestick injuries. The analysis of two open-label Phase I trials evaluated the pharmacokinetics, successful administration, and tolerability of peginterferon alfa-2a when using the autoinjector. The studies were performed to support the filing and registration of the autoinjector device.Methods: In trial 1, 50 healthy adult subjects received one 180 µg dose of peginterferon alfa-2a via the autoinjector. Serial blood samples were collected predose, up to 336 hours following drug administration, and at follow-up (28 ± 3 days post-dosing for noncompartmental pharmacokinetic analysis. Trial 2 randomized 60 adult patients with chronic hepatitis C to 180 µg peginterferon alfa-2a once weekly by the autoinjector or prefilled syringe for 3 weeks followed by the alternative device (prefilled syringe or autoinjector, respectively for 3 weeks. Patients also received ribavirin. Administration by the devices was evaluated under direct observation by a study staff member and by patient subjective assessment.Results: In trial 1, following a single dose of peginterferon alfa-2a, the maximum plasma concentration was 16.1 ± 5.3 ng/mL (mean ± standard deviation, and area under the concentration time curve (0–168 hours was 1996 ± 613 ng · hour

  5. Evaluating the transport layer of the ALFA framework for the Intel® Xeon Phi™ Coprocessor

    Science.gov (United States)

    Santogidis, Aram; Hirstius, Andreas; Lalis, Spyros

    2015-12-01

    The ALFA framework supports the software development of major High Energy Physics experiments. As part of our research effort to optimize the transport layer of ALFA, we focus on profiling its data transfer performance for inter-node communication on the Intel Xeon Phi Coprocessor. In this article we present the collected performance measurements with the related analysis of the results. The optimization opportunities that are discovered, help us to formulate the future plans of enabling high performance data transfer for ALFA on the Intel Xeon Phi architecture.

  6. Nebulised dornase alfa versus placebo or hypertonic saline in adult critically ill patients

    DEFF Research Database (Denmark)

    Claudius, Casper; Perner, Anders; Møller, Morten Hylander

    2015-01-01

    BACKGROUND: Nebulised dornase alfa is used off-label in critically ill patients. We aimed to assess the benefits and harms of nebulised dornase alfa versus placebo, no prophylaxis, or hypertonic saline on patient-important outcome measures in adult critically ill patients. METHODS: We performed a...... systematic review with meta-analysis and trial sequential analysis (TSA) using the Cochrane Collaboration methodology. Eligible trials were randomised clinical trials comparing nebulised dornase alfa with placebo, no prophylaxis, or hypertonic saline. The predefined outcome measures were all-cause mortality...

  7. Clearance of Hepatic Sphingomyelin by Olipudase Alfa Is Associated With Improvement in Lipid Profiles in Acid Sphingomyelinase Deficiency.

    Science.gov (United States)

    Thurberg, Beth L; Wasserstein, Melissa P; Jones, Simon A; Schiano, Thomas D; Cox, Gerald F; Puga, Ana Cristina

    2016-09-01

    Acid sphingomyelinase deficiency (ASMD; Niemann-Pick disease type A and B) is a lysosomal storage disorder characterized by abnormal intracellular sphingomyelin (SM) accumulation. Prominent liver involvement results in hepatomegaly, fibrosis/cirrhosis, abnormal liver chemistries, and a proatherogenic lipid profile. Olipudase alfa (recombinant human ASM) is in clinical development as an investigational enzyme replacement therapy for the non-neurological manifestations of ASMD. In a phase 1b study conducted to evaluate the safety and tolerability of within-patient dose escalation with olipudase alfa, measurement of SM levels in liver biopsies was used as a pharmacodynamic biomarker of substrate burden. Five adult patients with non neuronopathic ASMD received escalating doses of olipudase alfa every 2 weeks for 26 weeks. Liver biopsies obtained at baseline and 26 weeks after treatment were evaluated for SM storage by histomorphometric analysis, biochemistry, and electron microscopy. Biopsies were also assessed for inflammation and fibrosis, and for the association of SM levels with liver volume, liver function tests, and lipid profiles. At baseline, SM storage present in Kupffer cells and hepatocytes ranged from 9.8% to 53.8% of the microscopic field. After 26 weeks of treatment, statistically significant reductions in SM (P<0.0001) measured by morphometry were seen in 4 patients with evaluable liver biopsies. The 26-week biopsy of the fifth patient was insufficient for morphometric quantitation. Posttreatment SM levels ranged from 1.2% to 9.5% of the microscopic field, corresponding to an 84% to 92% relative reduction from baseline. Improvements in liver volume, liver function tests, and lipid profiles were also observed. This study illustrates the utility of SM assessment by liver biopsy as a pharmacodynamic biomarker of disease burden in these patients. PMID:27340749

  8. Comparing the effect and economic effectiveness of Interferon alfa-1b and Lamivudine in treating chronic hepatitis B%干扰素及拉米夫定治疗慢性乙肝成本-效果比较

    Institute of Scientific and Technical Information of China (English)

    何红; 余干林

    2002-01-01

    目的对比干扰素与拉米夫定治疗慢性乙肝的疗效及经济效果.方法将106例患者随机分为2组.一组应用干扰素α-1b治疗(60例),另一组应用拉米夫定治疗(46例).统计分析两组的疗效并进行经济学分析.结果与结论两组药物均能有效治疗慢性乙肝,干扰素疗效稍好,拉米夫定费用较低.

  9. Greater Epoetin alfa Responsiveness Is Associated With Improved Survival in Hemodialysis Patients

    OpenAIRE

    Kilpatrick, Ryan D.; Critchlow, Cathy W; Fishbane, Steven; Besarab, Anatole; Stehman-Breen, Catherine; Krishnan, Mahesh; Bradbury, Brian D.

    2008-01-01

    Background and objectives: Among hemodialysis patients, achieved hemoglobin is associated with Epoetin alfa dose and erythropoietin responsiveness. A prospective erythropoietin responsiveness measure was developed and its association with mortality evaluated.

  10. Alfa tahab TeliaSonerat Türgis üle pakkuda / Tõnis Arnover

    Index Scriptorium Estoniae

    Arnover, Tõnis, 1952-

    2005-01-01

    TeliaSoneraga, kellele kuulub 37 protsenti Türgi operaatorfirma Turkcell aktsiatest, konkureerib täiendava 27 protsendise aktsiapaki ostmises Venemaa operaatorfirmaga Alfa Telecom. Lisa: Kaasosanike huvid põrkuvad. Diagramm: Konkurendid Vene MegaFonis

  11. Combination of interferon alfa-2b and ribavirin in relapsed or nonresponding chronic hepatitis C patients following interferon therapy

    Directory of Open Access Journals (Sweden)

    Laurentius A. Lesmana

    2001-12-01

    Full Text Available Twenty six patients (pts with chronic hepatitis C (CHC who reLapsed or non-responded following.interferon (IFN therapy were given lFN alfa-2b 3   MIU three times a week for 48 weeks in combination with Ribavirin 800-1000 mg daily 2I (80,8% of the 26.pts completed the study consisted of 12 relapsers and 9 non-responders. Five pts dropped out due to drug adverse events in three pts and non-drug related reason in the other two. In the relapsed group complete response, relapse and sustained response rates were obtained in 9/12(75%, 2/2 (16,5% and 7/12(58,3% pts respectively. In the non- responding group, these figures were 3/9 (33,3%, 1/9(I1,1%, and 2/9(22,2% pts, respectively. The most frequent adverse event was flu-like syndrome, which was found in 18 pts (85,7%. Combination therapy of IFN alfa-2b and ribavirin may induce sustained virological response in relapsed and non-responding CHC patients. This combination therapy is more effective for relapsers compared to for non-responders. (Med J Indones 2001; 10: 214-8Keywords: Chronic hepatitis C, combination therapy, interferon, ribavirin

  12. Fotoquímica de alfa,alfa-dimetilvalerofenona adsorvida em celulose microcristalina

    Directory of Open Access Journals (Sweden)

    Behrens Maria das Dores D.

    2006-01-01

    Full Text Available Irradiation of a,a-dimethylvalerophenone (1 adsorbed on microcrystalline cellulose employing methanol as the solvent shows a Norrish Type II/Type I ratio of 1.0?0.1. In solution, values of 2.3?0.3 in benzene and 8.7?2.0 in terc-butanol were obtained. The cyclization/elimination ratio for the Norrish Type II reaction of 1 shows values of 1.2?0.3 in cellulose, 17.9?2.7 in benzene and 3.2?03 in terc-butanol. When samples of 1/microcrystalline cellulose were prepared employing n-hexane, the Type II/Type I (29.5?2.9 and the cycl/elim (113.3?12.1 ratios were dramatically modified. These results demonstrate the difference in the behavior of 1 when entrapped in the cellulose chains or adsorbed on the cellulose surface.

  13. Performance evaluation and optimization of the luminosity detector ALFA

    CERN Document Server

    Jakobsen, Sune; Grafström, P; Joram, C

    2010-01-01

    The startup of the LHC (Large Hadron Collider) has initialized a new era in particle physics. The standard model of particle physics has for the last 40 years with tremendous success described all measurements with phenomenal precision. The experiments at the LHC will test the standard model in a new energy regime. To normalize the measurements and understand the potential discoveries of the LHC experiments it is often crucial to know the interaction rate - the absolute luminosity. The ATLAS (A Toroidal LHC ApparatuS) detector will measure luminosity by numerous methods. But for most of the methods only the relative luminosity is measured with good precision. The absolute scale has to be provided from elsewhere. Therefore ATLAS plans to measure the flux of protons scattered under very small angles as this flux relates directly and with good precision to the absolute luminosity. This will be done by the ALFA (Absolute Luminosity For ATLAS) detector. The detectors will be positioned about 240 m from the interac...

  14. Corifollitropin alfa followed by rFSH in a GnRH antagonist protocol for poor ovarian responder patients

    DEFF Research Database (Denmark)

    Polyzos, Nikolaos P; Devos, Michel; Humaidan, Peter;

    2013-01-01

    developed by European Society for Human Reproduction and Embryology Consensus Group. INTERVENTION(S): Corifollitropin alfa (150 μg) followed by 300 IU rFSH in a GnRH antagonist protocol. MAIN OUTCOME MEASURE(S): Endocrinologic profile and ongoing pregnancy rates. RESULT(S): Among 43 women treated with...... corifollitropin alfa, mean E(2) levels showed an increasing pattern during the follicular phase, reaching 825 ng/L on the day of hCG administration, whereas FSH values showed a marked increase during the first 5 days, reaching a mean value of 35 IU/L and remaining above 20 IU/L during the late follicular phase....... Cycle cancellation rate was 32.6% and embryo transfer rate 53.3%. Five patients (11.7%) had a positive hCG test and three (7%) had an ongoing pregnancy. Ongoing pregnancy rates were 11.1% per oocyte retrieval and 13% per embryo transfer. Ongoing pregnancy rates per patient did not significantly differ...

  15. Baseline characteristics of patients in the Reduction of Events with Darbepoetin alfa in Heart Failure trial (RED-HF)

    Science.gov (United States)

    McMurray, John J.V.; Anand, Inder S.; Diaz, Rafael; Maggioni, Aldo P.; O'Connor, Christopher; Pfeffer, Marc A.; Solomon, Scott D.; Tendera, Michal; van Veldhuisen, Dirk J.; Albizem, Moetaz; Cheng, Sunfa; Scarlata, Debra; Swedberg, Karl; Young, James B.; Amuchastegui, M.; Belziti, C.; Bluguermann, J.; Caccavo, M.; Cartasegna, L.; Colque, R.; Cuneo, C.; Fernandez, A.; Gabito, A.; Goicochea, R.; Gonzalez, M.; Gorosito, V.; Grinfeld, L.; Hominal, M.; Kevorkian, R.; Litvak Bruno, M.; Llanos, J.; Mackinnon, I.; Manuale, O.; Marzetti, E.; Nul, D.; Perna, E.; Riccitelli, M.; Sanchez, A.; Santos, D.; Schygiel, P.; Toblli, J.; Vogel, D.; Aggarwal, A.; Amerena, J.; De Looze, F.; Fletcher, P.; Hare, D.; Ireland, M.; Krum, H.; Lattimore, J.; Marwick, T.; Sindone, A.; Thompson, P.; Waites, J.; Altenberger, J.; Ebner, C.; Lenz, K.; Pacher, R.; Poelzl, G.; Charlier, F.; de Ceuninck, M.; De Keulenaer, G.; Dendale, P.; Maréchal, P.; Mullens, W.; Thoeng, J.; Vanderheyden, M.; Vanhaecke, J.; Weytjens, C.; Wollaert, B.; Albuquerque, D.; Almeida, D.; Aspe y Rosas, J.; Bocchi, E.; Bordignon, S.; Clausell, N.; Kaiser, S.; Leaes, P.; Martins Alves, S.; Montera, M.; Moura, L.; Pereira de Castro, R.; Rassi, S.; Reis, A.; Saraiva, J.; Simões, M.; Souza Neto, J.; Teixeira, M.; Benov, H.; Chompalova, B.; Donova, T.; Georgiev, P.; Gotchev, D.; Goudev, A.; Grigorov, M.; Guenova, D.; Hergeldjieva, V.; Ivanov, D.; Kostova, E.; Manolova, A.; Marchev, S.; Nikolov, F.; Popov, A.; Raev, D.; Tzekova, M.; Czarnecki, W.; Giannetti, N.; Haddad, H.; Heath, J.; Huynh, T.; Lepage, S.; Liu, P.; Lonn, E.; Ma, P.; Manyari, D.; Moe, G.; Parker, J.; Pesant, Y.; Rajda, M.; Ricci, J.; Roth, S.; Sestier, F.; Sluzar, V.; Sussex, B.; Vizel, S.; Antezana, G.; Bugueno, C.; Castro, P.; Conejeros, C.; Manriquez, L.; Martinez, D.; Potthoff, S.; Stockins, B.; Vukasovic, J.; Gregor, P.; Herold, M.; Jerabek, O.; Jirmar, R.; Kuchar, R.; Linhart, A.; Podzemska, B.; Soucek, M.; Spac, J.; Spacek, R.; Vodnansky, P.; Bronnum-Schou, J.; Clemmensen, K.; Egstrup, K.; Jensen, G.; Kjoller-Hansen, L.; Kober, L.; Markenvard, J.; Rokkedal, J.; Skagen, K.; Torp-Pedersen, C.; Tuxen, C.; Videbak, L.; Laks, T.; Vahula, V.; Harjola, V.; Kettunen, R.; Kotila, M.; Bauer, F.; Cohen Solal, A.; Coisne, D.; Davy, J.; De Groote, P.; Dos Santos, P.; Funck, F.; Galinier, M.; Gibelin, P.; Isnard, R.; Neuder, Y.; Roul, G.; Sabatier, R.; Trochu, J.; Anker, S.; Denny, S.; Dreykluft, T.; Flesch, M.; Genth-Zotz, S.; Hambrecht, R.; Hein, J.; Jeserich, M.; John, M.; Kreider-Stempfle, H.; Laufs, U.; Muellerleile, K.; Natour, M.; Sandri, M.; Schäufele, T.; von Hodenberg, E.; Weyland, K.; Winkelmann, B.; Tse, H.; Yan, B.; Barsi, B.; Csikasz, J.; Dezsi, C.; Edes, I.; Forster, T.; Karpati, P.; Kerekes, C.; Kis, E.; Kosa, I.; Lupkovics, G.; Nagy, A.; Preda, I.; Ronaszeki, A.; Tomcsanyi, J.; Zamolyi, K.; Agarwal, D.; Bahl, V.; Bordoloi, A.; Chockalingam, K.; Chopda, M.; Chopra, V.; Dugal, J.; Ghaisas, N.; Ghosh, S.; Grant, P.; Hiremath, S.; Iyengar, S.; Jagadeesa Subramania, B.; Jain, P.; Joshi, A.; Khan, A.; Mullasari, A.; Naik, S.; Oomman, A.; Pai, V.; Pareppally Gopal, R.; Parikh, K.; Patel, T.; Prakash, V.; Sastry, B.; Sathe, S.; Sinha, N.; Srikanthan, V.; Subburamakrishnan, P.; Thacker, H.; Wander, G.; Admon, D.; Katz, A.; Klainman, E.; Lewis, B.; Marmor, A.; Moriel, M.; Mosseri, M.; Shotan, A.; Weinstein, J.; Zimlichman, R.; Agostoni, P.; Albanese, M.; Alunni, G.; Bini, R.; Boccanelli, A.; Bolognese, L.; Campana, C.; Carbonieri, E.; Carpino, C.; Checco, L.; Cosmi, F.; D'Angelo, G.; De Cristofaro, M.; Floresta, A.; Fucili, A.; Galvani, M.; Ivleva, A.; Marra, S.; Musca, G.; Peccerillo, N.; Perrone Filardi, P.; Picchio, E.; Russo, T.; Scelsi, L.; Senni, M.; Tavazzi, L.; Erglis, A.; Jasinkevica, I.; Kakurina, N.; Veze, I.; Volans, E.; Bagdonas, A.; Berukstis, E.; Celutkiene, J.; Dambrauskaite, A.; Jarasuniene, D.; Luksiene, D.; Rudys, A.; Sakalyte, G.; Sliaziene, S.; Aguilar-Romero, R.; Cardona-Muñoz, E.; Castro-Jimenez, J.; Chavez-Herrera, J.; Chuquiure Valenzuela, E.; De la Pena, G.; Herrera, E.; Leiva-Pons, J.; Lopez Alvarado, A.; Mendez Machado, G.; Ramos-Lopez, G.; Basart, D.; Buijs, E.; Cornel, J.; de Leeuw, M.; Dijkgraaf, R.; Dunselman, P.; Freericks, M.; Hamraoui, K.; Lenderlink, T.; Linssen, G.; Lodewick, P.; Lodewijks, C.; Lok, D.; Nierop, P.; Ronner, E.; Somsen, A.; van Dantzig, J.; van der Burgh, P.; van Kempen, L.; van Vlies, B.; Voors, A.; Wardeh, A.; Willems, F.; Dickstein, K.; Gundersen, T.; Hole, T.; Thalamus, J.; Westheim, A.; Dabrowski, M.; Gorski, J.; Korewicki, J.; Kuc, K.; Miekus, P.; Musial, W.; Niegowska, J.; Piotrowski, W.; Podolec, P.; Polonski, L.; Ponikowski, P.; Rynkiewicz, A.; Szelemej, R.; Trusz-Gluza, M.; Ujda, M.; Wojciechowski, D; Wysokinski, A.; Camacho, A.; Fonseca, C.; Monteiro, P.; Apetrei, E.; Bruckner, I.; Carasca, E.; Coman, I.; Datcu, M.; Dragulescu, S.; Ionescu, P.; Iordachescu-Petica, D.; Manitiu, I.; Popa, V.; Pop-Moldovan, A.; Radoi, M.; Stamate, S.; Tomescu, M.; Vita, I.; Aroutiounov, G.; Ballyuzek, M.; Bart, B.; Churina, S.; Glezer, M.; Goloshchekin, B.; Ivleva, A.; Kobalava, Z.; Kostenko, V.; Lopatin, Y.; Martynov, A.; Orlov, V.; Semernin, E.; Shogenov, Z.; Sidorenko, B.; Skvortsov, A.; Storzhakov, G.; Sulimov, V.; Talibov, O.; Tereshenko, S.; Tsyrline, V.; Zadionchenko, V.; Zateyshchikov, D.; Dzupina, A.; Hranai, M.; Kmec, J.; Micko, K.; Murin, J.; Pella, D.; Sojka, G.; Spisak, V.; Vahala, P.; Vinanska, D.; Badat, A.; Bayat, J.; Dawood, S.; Delport, E.; Ellis, G.; Garda, R.; Klug, E.; Mabin, T.; Naidoo, D.; Pretorius, M.; Ranjith, N.; Van Zyl, L.; Weich, H.; Anguita, M.; Berrazueta, J.; Bruguera i Cortada, J.; de Teresa, E.; Gómez Sánchez, M.; González Juanatey, J.; Gonzalez-Maqueda, I.; Jordana, R.; Lupon, J.; Manzano, L.; Pascual Figal, D.; Pulpón, L.; Recio, J.; Ridocci Soriano, F.; Rodríguez Lambert, J.; Roig Minguell, E.; Roig Minguell, E.; Romero, J.; Valdovinos, P.; Klintberg, L.; Kronvall, T.; Lycksell, M.; Morner, S.; Rydberg, E.; Swedberg, K.; Timberg, I.; Wikstrom, G.; Moccetti, T.4; Ashok, J.; Banerjee, P.; Carr-White, G.; Cleland, J.; Connolly, E.; Francis, M.; Greenbaum, R.; Kadr, H.; Lindsay, S.; McMurray, J.; Megarry, S.; Memon, A.; Murdoch, D.; Senior, R.; Squire, I.; Tan, L.; Witte, K.; Adams, K.; Adamson, P.; Adler, A.; Altschul, L.; Altschuller, A.; Amirani, H.; Anand, I.; Andreou, C.; Ansari, M.; Antonishen, M.; Banchs, H.; Banerjee, S.; Banish, D.; Bank, A.; Barbagelata, A.; Barnard, D.; Bellinger, R.; Benn, A.; Berk, M.; Berry, B.; Bethala, V.; Bilazarian, S.; Bisognano, J.; Bleyer, F.; Blum, M.; Boehmer, J.; Bouchard, A.; Boyle, A.; Bozkurt, B.; Brown, C.; Burlew, B.; Burnham, K.; Butler, J.; Call, J.; Cambier, P.; Cappola, T.; Carlson, R.; Chandler, B.; Chandra, R.; Chandraratna, P.; Chernick, R.; Colan, D.; Colfer, H.; Colucci, W.; Connelly, T.; Costantini, O.; Dadkhah, S.; Dauber, I.; Davis, J.; Davis, S.; Denning, S.; Drazner, M.; Dunlap, S.; Egbujiobi, L.; Elkayam, U.; Elliott, J.; El-Shahawy, M.; Essandoh, L.; Ewald, G.; Fang, J.; Farhoud, H.; Felker, G.; Fernandez, J.; Festin, R.; Fishbein, G.; Florea, V.; Flores, E.; Floro, J.; Gabris, M.; Garg, M.; Gatewood, R.; Geller, M.; Ghali, J.; Ghumman, W.; Gibbs, G.; Gillespie, E.; Gilmore, R.; Gogia, H.; Goldberg, L.; Gradus-Pizlo, I.; Grainger, T.; Gudmundsson, G.; Gunawardena, D.; Gupta, D.; Hack, T.; Hall, S.; Hamroff, G.; Hankins, S.; Hanna, M.; Hargrove, J.; Haught, W.; Hauptman, P.; Hazelrigg, M.; Herzog, C.; Heywood, J.; Hill, T.; Hilton, T.; Hirsch, H.; Hunter, J.; Ibrahim, H.; Imburgia, M.; Iteld, B.; Jackson, B.; Jaffrani, N.; Jain, D.; Jain, A.; James, M.; Jimenez, J.; Johnson, E.; Kale, P.; Kaneshige, A.; Kapadia, S.; Karia, D.; Karlsberg, R.; Katholi, R.; Kerut, E.; Khoury, W.; Kipperman, R.; Klapholz, M.; Kosinski, E.; Kozinn, M.; Kraus, D.; Krueger, S.; Krum, H.; Kumar, S.; Lader, E.; Lee, C.; Levy, W.; Lewis, E.; Light-McGroary, K.; Loh, I.; Lombardi, W.; Machado, C.; Maislos, F.; Mancini, D.; Markus, T.; Mather, P.; McCants, K.; McGrew, F.; McLaurin, B.; McMillan, E.; McNamara, D.; Meyer, T.; Meymandi, S.; Miller, A.; Minami, E.; Modi, M.; Mody, F.; Mohanty, P.; Moscoso, R.; Moskowitz, R.; Moustafa, M.; Mullen, M.; Naz, T.; Noonan, T.; O'Brien, T.; Oellerich, W.; Oren, R.; Pamboukian, S.; Pereira, N.; Pitt, W.; Porter, C.; Prabhu, S.; Promisloff, S.; Ratkovec, R.; Richardson, R.; Ross, A.; Saleh, N.; Saltzberg, M.; Sarkar, S.; Schmedtje, J.; Schneider, R.; Schuyler, G.; Shanes, J.; Sharma, A.; Siegel, C.; Siegel, R.; Silber, D.; Singh, V.; Singh, N.; Singh, J.; Sklar, J.; Small, R.; Smith, A.; Smith, E.; Smith, E.; Smull, D.; Sotolongo, R.; Staniloae, C.; Stapleton, D.; Steele, P.; Stehlik, J.; Stein, M.; Tang, W.; Thadani, U.; Torre-Amoine, G.; Trichon, B.; Tsai, C.; Tummala, R.; Van Bakel, A.; Vicari, R.; Vijay, N.; Vijayaraghavan, K.; Vittorio, T.; Vossler, M.; Wagoner, L.; Wallis, D.; Ward, N.; Widmer, M.; Wight, J.; Wilkins, C.; Williams, C.; Williams, G.; Winchester, M.; Winkel, E.; Wittmer, B.; Wood, D.; Wormer, D.; Wright, R.; Xu, Z.; Yasin, M.; Zolty, R.

    2013-01-01

    Aims This report describes the baseline characteristics of patients in the Reduction of Events with Darbepoetin alfa in Heart Failure trial (RED-HF) which is testing the hypothesis that anaemia correction with darbepoetin alfa will reduce the composite endpoint of death from any cause or hospital admission for worsening heart failure, and improve other outcomes. Methods and results Key demographic, clinical, and laboratory findings, along with baseline treatment, are reported and compared with those of patients in other recent clinical trials in heart failure. Compared with other recent trials, RED-HF enrolled more elderly [mean age 70 (SD 11.4) years], female (41%), and black (9%) patients. RED-HF patients more often had diabetes (46%) and renal impairment (72% had an estimated glomerular filtration rate <60 mL/min/1.73 m2). Patients in RED-HF had heart failure of longer duration [5.3 (5.4) years], worse NYHA class (35% II, 63% III, and 2% IV), and more signs of congestion. Mean EF was 30% (6.8%). RED-HF patients were well treated at randomization, and pharmacological therapy at baseline was broadly similar to that of other recent trials, taking account of study-specific inclusion/exclusion criteria. Median (interquartile range) haemoglobin at baseline was 112 (106–117) g/L. Conclusion The anaemic patients enrolled in RED-HF were older, moderately to markedly symptomatic, and had extensive co-morbidity. PMID:23329651

  16. Collaborative study for the validation of an improved HPLC assay for recombinant IFN-alfa-2.

    Science.gov (United States)

    Jönsson, K H; Daas, A; Buchheit, K H; Terao, E

    2016-01-01

    The current European Pharmacopoeia (Ph. Eur.) texts for Interferon (IFN)-alfa-2 include a nonspecific photometric protein assay using albumin as calibrator and a highly variable cell-based assay for the potency determination of the protective effects. A request was expressed by the Official Medicines Control Laboratories (OMCLs) for improved methods for the batch control of recombinant interferon alfa-2 bulk and market surveillance testing of finished products, including those formulated with Human Serum Albumin (HSA). A HPLC method was developed at the Medical Products Agency (MPA, Sweden) for the testing of IFN-alfa-2 products. An initial collaborative study run under the Biological Standardisation Programme (BSP; study code BSP039) revealed the need for minor changes to improve linearity of the calibration curves, assay reproducibility and robustness. The goal of the collaborative study, coded BSP071, was to transfer and further validate this improved HPLC method. Ten laboratories participated in the study. Four marketed IFN-alfa-2 preparations (one containing HSA) together with the Ph. Eur. Chemical Reference Substance (CRS) for IFN-alfa-2a and IFN-alfa-2b, and in-house reference standards from two manufacturers were used for the quantitative assay. The modified method was successfully transferred to all laboratories despite local variation in equipment. The resolution between the main and the oxidised forms of IFN-alfa-2 was improved compared to the results from the BSP039 study. The improved method even allowed partial resolution of an extra peak after the principal peak. Symmetry of the main IFN peak was acceptable for all samples in all laboratories. Calibration curves established with the Ph. Eur. IFN-alfa-2a and IFN-alfa-2b CRSs showed excellent linearity with intercepts close to the origin and coefficients of determination greater than 0.9995. Assay repeatability, intermediate precision and reproducibility varied with the tested sample within acceptable

  17. Cloning and expression of codon-optimized recombinant darbepoetin alfa in Leishmania tarentolae T7-TR.

    Science.gov (United States)

    Kianmehr, Anvarsadat; Golavar, Raziyeh; Rouintan, Mandana; Mahrooz, Abdolkarim; Fard-Esfahani, Pezhman; Oladnabi, Morteza; Khajeniazi, Safoura; Mostafavi, Seyede Samaneh; Omidinia, Eskandar

    2016-02-01

    Darbepoetin alfa is an engineered and hyperglycosylated analog of recombinant human erythropoietin (EPO) which is used as a drug in treating anemia in patients with chronic kidney failure and cancer. This study desribes the secretory expression of a codon-optimized recombinant form of darbepoetin alfa in Leishmania tarentolae T7-TR. Synthetic codon-optimized gene was amplified by PCR and cloned into the pLEXSY-I-blecherry3 vector. The resultant expression vector, pLEXSYDarbo, was purified, digested, and electroporated into the L. tarentolae. Expression of recombinant darbepoetin alfa was evaluated by ELISA, reverse-transcription PCR (RT-PCR), Western blotting, and biological activity. After codon optimization, codon adaptation index (CAI) of the gene raised from 0.50 to 0.99 and its GC% content changed from 56% to 58%. Expression analysis confirmed the presence of a protein band at 40 kDa. Furthermore, reticulocyte experiment results revealed that the activity of expressed darbepoetin alfa was similar to that of its equivalent expressed in Chinese hamster ovary (CHO) cells. These data suggested that the codon optimization and expression in L. tarentolae host provided an efficient approach for high level expression of darbepoetin alfa. PMID:26546410

  18. Characterization of autoantibodies in autoimmune hemolytic anemia following treatment with interferon alfa

    International Nuclear Information System (INIS)

    We studied 13 patients with chronic myeloid leukemia and autoimmune hemolytic anemia induced by interferon alfa. They underwent tests for immune protein detection and characterization of IgG subclasses in RBCs by direct antiglobulin test (PAD) and the microplate technique. Also they were applied ELISA test for quantifying immunoglobulins in the red blood cells. It was detected the presence of IgG and C3 in 53.84 % of cases, IgG alone in 23.07 % and in 15.38 % were identified IgG and IgA autoantibodies. In 11 patients the presence of IgG1 was showed and also in one case the subclass IgG3 autoantibodies was identified. The ELISA detected antibodies at concentrations of 183 IgG molecules per erythrocyte in a patient with negative PAD. In high-grade hemolysis patients, it was found a concentration of autoantibodies between 1 500 and 3 180 molecules of IgG per erythrocyte, while in low-grade hemolysis patients it behaved between 183 and 1 000 molecules. There was a negative correlation between Hb and plasma haptoglobin values with the number of IgG molecules per erythrocyte and a positive correlation between the latter with the reticulocyte count

  19. Possibilities of uranium recovering as by product of copper lixiviation solution in Salobo 3-Alfa-Carajas

    International Nuclear Information System (INIS)

    Geochemical and geophysics regional surveys performed by Nuclebras in 'Carajas, Para' State, show several favourable areas for uranium prospecting, including the 'Salobo 3-Alfa' copper mine. Some studies for increasing the Knowledge of uranium quantity and proportion and for accompanying the pilot plant installation, aiming the uranium extraction during the cycel of ore treatment in 'Salobo 3-Alfa' mine are described

  20. Tratamento de hemangioma gigante com interferon alfa: relato de dois casos Treatment of giant hemangioma with interferon-alpha: report of two cases

    Directory of Open Access Journals (Sweden)

    Ana Julia Balau

    2007-12-01

    Full Text Available O objetivo do trabalho é descrever o uso de interferon alfa no tratamento de pacientes com hemangioma gigante. Os autores relatam e analisam dois casos de hemangioma gigante em tratamento com interferon alfa. IBS, 3 anos, em acompanhamento no Ambulatório de Hematologia desde um ano de idade com quadro de lesão angiomatosa em praticamente toda hemiface direita, acompanhada de sangramentos gengivais importantes. Após a realização de exames complementares (Angiorressonância magnética e feito o diagnóstico de hemangioma gigante em face, foi iniciado tratamento com prednisona e, posteriormente, associação com interferon alfa e observada importante melhora do quadro, resultando na diminuição dos episódios de sangramento e no tamanho do tumor. C.N.P., 12 anos, apresentando nódulo em região lateral de joelho esquerdo há 2 anos, com aumento progressivo do tamanho e dor local. Fez uso de prednisona e, sem melhora do quadro, introduzido interferon alfa com regressão importante do tamanho do tumor. O tratamento com interferon alfa deve ser considerado no tratamento de hemangiomas, pois apresenta bons resultados em relação à diminuição do tamanho do tumor e, conseqüentemente, reduz as intercorrências clínicas associadas à sua presença, principalmente os sangramentos.The aim of this study is to describe the treatment using interferon-alpha of giant hemangiomas in children. The authors report two cases of children presenting with giant hemangiomas treated using interferon-alpha and analyze the results. IBS, 3 years-old, has been followed up in Famema Hemathology Service since she was 1 year-old with a tumor on the face and persistent bleeding. After clinical and radiologic evaluations and suggested the diagnosis of giant hemangioma, she started treatment with interferon-alpha. A great clinical improvement was observed a reducing of the number of episodes of bleedings and a decrease in of the tumor size. CNP, 12 years-old, came to

  1. LHC Report: special run with de-squeezed beams for ATLAS/ALFA and TOTEM

    CERN Multimedia

    Helmut Burkhardt for the LHC team

    2015-01-01

    The main high-luminosity proton-proton run of the LHC is complemented by one week per year of special proton-proton runs. The special runs are performed with larger beam sizes at the interaction points to allow the forward physics experiments, TOTEM and ATLAS/ALFA, the chance to make precise measurements of protons as they emerge from collisions at small angles.   In standard high-luminosity operation, the beams are squeezed to give small beam sizes at the interaction points to maximise the collision rates. The “squeeze” takes place at top energy and the beam size at the centre of ATLAS (IP1) and CMS (IP5) is reduced from 66 micrometres at the top of the ramp to 18 micrometres before colliding beams are established. Protons that avoid the fate of an inelastic collision but yet still interact – in elastic or diffractive events – are scattered and emerge in the forward direction. The reduction in beam size has a side effect of increasing the an...

  2. Arecibo Pulsar Survey Using ALFA. III. Precursor Survey and Population Synthesis

    CERN Document Server

    Swiggum, J K; McLaughlin, M A; Bates, S D; Champion, D J; Ransom, S M; Lazarus, P; Brazier, A; Hessels, J W T; Nice, D J; Ellis, J; Senty, T R; Allen, B; Bhat, N D R; Bogdanov, S; Camilo, F; Chatterjee, S; Cordes, J M; Crawford, F; Deneva, J S; Freire, P C C; Jenet, F A; Karako-Argaman, C; Kaspi, V M; Knispel, B; Lee, K J; Van Leeuwen, J; Lynch, R; Lyne, A G; Scholz, P; Siemens, X; Stairs, I H; Stappers, B W; Stovall, K; Venkataraman, A; Zhu, W W

    2014-01-01

    The Pulsar Arecibo L-band Feed Array (PALFA) Survey uses the ALFA 7-beam receiver to search both inner and outer Galactic sectors visible from Arecibo ($32^{\\circ}\\lesssim \\ell \\lesssim 77^{\\circ}$ and $168^{\\circ}\\lesssim \\ell \\lesssim 214^{\\circ}$) close to the Galactic plane ($|b|\\lesssim5^{\\circ}$) for pulsars. In this paper we detail a precursor survey of this region with PALFA, which observed a subset of the full region (slightly more restrictive in $\\ell$ and $|b|\\lesssim1^{\\circ}$) and detected 45 pulsars. For both Galactic millisecond and normal pulsar populations, we compare the survey's detections with simulations to model these populations and, in particular, to estimate the number of observable pulsars in the Galaxy. We place 95\\% confidence intervals of 82,000 to 143,000 on the number of detectable normal pulsars and 9,000 to 100,000 on the number of detectable millisecond pulsars in the Galactic disk. These are consistent with previous estimates. Given the most likely population size in each ca...

  3. Cholangiolocellular carcinoma with rapid progression initially showing abnormally elevated serum alfa-fetoprotein.

    Science.gov (United States)

    Yoh, Tomoaki; Kato, Tatsushi; Hirohata, Yoshiaki; Nakamura, Yuya; Nakayama, Hiroyuki; Okamura, Ryuji

    2016-08-01

    Cholangiolocellular carcinoma (CoCC) is a rare malignant liver tumor derived from hepatic progenitor cells, which exist in the canals of Hering. We encountered a case of CoCC with an extremely poor clinical course, initially showing abnormally elevated serum alfa-fetoprotein (AFP). A 72-year-old male presented with a liver tumor and abnormally elevated serum AFP levels (16,399 ng/ml). We preoperatively diagnosed hepatocellular carcinoma and performed extended right hepatectomy, after which the serum AFP levels remarkably decreased to 97 ng/ml. Postoperatively, the disease was pathologically diagnosed as CoCC. Furthermore, immunohistochemical pathological findings were alcian blue negative, cytokeratin (CK) 7 partially positive, CK19 positive, hepatocyte paraffin-1 negative, membranous negative for epithelial membrane antigen, and AFP negative. Fifty-five days later, intra- and extrahepatic recurrence developed, and the patient died 65 days after surgery. Although CoCCs show favorable outcomes, these characteristics of our case were not previously reported. It is necessary to accumulate more information on CoCC. PMID:27363839

  4. Peg-Interferon Alfa 2-b Related Cellulitis in a 40 Years Man

    Directory of Open Access Journals (Sweden)

    Shahnaaz Sali

    2016-01-01

    Full Text Available Background: Pegylated interferon and ribavirin are currently one of the accepted treatment for chronic Hepatitis C. Dermatologic complications of interferon have been reported, but to date a few cases of bacterial cellulitis; a rare and severe complication, have been published. Cellulitis is a common infectious process affecting the skin and subcutaneous tissues which results in significant morbidity and holds considerable healthcare costs.Cases Report: Herein, we report a case of chronic hepatitis C genotype 1a who was on medication since 8 weeks prior to developing leg cellulitis, an uncommon pegylated interferon injection site. Considering no other possible risk factors were found to be in favor of bacterial cellulitis, our case is unique in its kind. Some reports reveal necrotizing vasculitis as basis for cutaneous lesions, which could be due to the high concentrations of drug at the injection site, a toxic effect of the diluents, or an immunological reaction.Conclusion: According to the latter mechanism patients could develop bacterial cellulitis in their different organs. Conclusively, we propose the hypothesis of a possible association between cellulitis to occur at any site as the complication of pegylated interferon Alfa 2b and would highlight the role of a careful skin examination that could be an asset in preventing local skin infections.

  5. LCDM Satellites and HI Companions - The Arecibo ALFA Survey of NGC 2903

    OpenAIRE

    Irwin, J. A.; Hoffman, G.L.; Spekkens, K.; Haynes, M. P.; Giovanelli, R.; Linder, S. M.; Catinella, B.; Momjian, E.; Koribalski, B. S.; Davies, J; Brinks, E.; de Blok, W. J. G.; Putman, M. E.; Van Driel, W.

    2008-01-01

    We have conducted a deep, complete HI survey, using Arecibo/ALFA, of a field centered on the nearby, isolated galaxy, NGC 2903, which is similar to the Milky Way in its properties. The field size was 150 kpc x 260 kpc and the final velocity range spanned from 100 to 1133 km/s. The ALFA beams have been mapped as a function of azimuth and cleaned from each azimuth-specific cube prior to forming final cubes. The final HI data are sensitive down to an HI mass of 2 x 10^5 Mo and column density of ...

  6. ESTABILIZACIÓN DE HOLO-alfa-LACTOALBÚMINA EN PRESENCIA DE POLIOLES

    Directory of Open Access Journals (Sweden)

    Alberto Albis

    2010-02-01

    Full Text Available En este trabajo se presenta un estudio sistemático sobre el efecto de las soluciones acuosas de eritritol, xilitol, sorbitol e inositol con diferentes concentraciones, sobre la estabilidad térmica de la holo- alfa-lactoalbúmina bovina con pH 6,5 usando espectroscopia UV-VIS. Los resultados obtenidos muestran que los polioles usados estabilizan la holo-alfa-lactoalbúmina en un grado significativamente menor al reportado para otras proteínas. Se sugiere que este menor efecto de estabilización ocurre debido a que esta proteína presenta un estado desnaturalizado parcialmente desdoblado.

  7. The ATLAS ALFA and AFP detectors - the experimental challenge of measuring forward protons at the LHC

    CERN Document Server

    Trzebinski, M; The ATLAS collaboration

    2013-01-01

    The Absolute Luminosity For ATLAS (ALFA) and ATLAS Forward Protons (AFP) detectors are introduced. The forward proton trajectories are shown. The similarities and differences between these two detector systems are described. Finally, the physics possible to be done in these forward detectors is discussed. In particular, in case of ALFA the elastic scattering and exclusive di-pion production are described. In case of AFP, the hard diffractive processes like: Single Diffractive Jet, Double Pomeron Exchange Jet, Exclusive Jet and anomalous coupling production are described.

  8. Hemoglobinas AS/alfa talassemia: importância diagnóstica Hemoglobins AS/alpha thalassemia: diagnostic importance

    Directory of Open Access Journals (Sweden)

    Renata Tomé-Alves

    2000-12-01

    Full Text Available Portadores de traço falciforme (hemoglobina AS associados a talassemia alfa apresentam alterações na morfologia dos eritrócitos, normalmente ausentes nos heterozigotos para esta variante de hemoglobina. A interação entre hemoglobina S e talassemia alfa tem sido descrita como um dos fatores responsáveis pela melhora no quadro clínico de portadores homozigotos de hemoglobina S (anemia falciforme, diminuindo os episódios de crises de falcização. Os mecanismos genéticos desta influência são avaliados em análises moleculares dos genes da globina humana. Com o objetivo de verificar a presença de talassemia alfa em portadores de hemoglobina S em heterozigose, com presença de anemia, encaminhados ao Laboratório de Hemoglobinas do Departamento de Biologia da UNESP, de São José do Rio Preto, analisamos 1.002 amostras de sangue com traço falciforme, no período de 1990 a 1998. As amostras foram colhidas com EDTA como anticoagulante, após prévia autorização dos portadores. Para o diagnóstico laboratorial as amostras de sangue foram submetidas a procedimentos eletroforéticos em pH alcalino e ácido e pesquisa citológica de hemoglobina H. Os procedimentos eletroforéticos confirmaram a presença de hemoglobina AS. A pesquisa citológica evidenciou a presença de talassemia alfa. Deste total analisado, 16 (1,59 % amostras de sangue apresentaram a associação entre hemoglobina AS e alfa talassemia, sendo que duas eram de indivíduos de uma mesma família. Os resultados obtidos nos direcionaram a sugerir aos laboratórios de rotina que realizem a pesquisa de alfa talassemia entre os portadores de hemoglobina AS, com presença de anemia, para verificar a interação com talassemia alfa, fornecendo assim aos portadores informação importante sobre seu perfil hematológico.Sickle Cell disease is a generic term for a group of genetic disorders characterized by the predominance of hemoglobin S. These disorders include Sickle Cell anemia

  9. Pericarditis and chronic inflammatory demyelinating polyneuropathy during therapy with pegylated interferon alfa-2a for chronic hepatitis C.

    Science.gov (United States)

    Nishio, Kazuaki; Konndo, Takeshi; Okada, Shunichi; Enchi, Machiko

    2010-09-27

    We report a case of pericarditis and chronic inflammatory demyelinating polyneuropathy with biological signs of a lupus-like syndrome due to pegylated interferon alfa-2a therapy during treatment for chronic hepatitis C. The patient developed moderate weakness in the lower limbs and dyspnea. He was hospitalized for congestive heart failure. An electrocardiogram showed gradual ST-segment elevation in leads V(1) through V(6) without coronary artery disease. A transthoracic cardiac ultrasonographic study revealed moderate pericardial effusion with normal left ventricular function. Anti-DNA antibody and antids DNA IgM were positive. Neurological examination revealed a symmetrical predominantly sensory polyneuropathy with impairment of light touch and pin prick in globe and stoking-like distribution. Treatment with prednisolone improved the pericarditis and motor nerve disturbance and the treatment with intravenous immunoglobulin improved the sensory nerve disturbance. PMID:21161021

  10. A Trial of Darbepoetin Alfa in Type 2 Diabetes and Chronic Kidney Disease

    NARCIS (Netherlands)

    Pfeffer, Marc A.; Burdmann, Emmanuel A.; Chen, Chao-Yin; Cooper, Mark E.; de Zeeuw, Dick; Eckardt, Kai-Uwe; Feyzi, Jan M.; Ivanovich, Peter; Kewalramani, Reshma; Levey, Andrew S.; Lewis, Eldrin F.; McGill, Janet B.; McMurray, John J. V.; Parfrey, Patrick; Parving, Hans-Henrik; Remuzzi, Giuseppe; Singh, Ajay K.; Solomon, Scott D.; Toto, Robert

    2009-01-01

    BACKGROUND Anemia is associated with an increased risk of cardiovascular and renal events among patients with type 2 diabetes and chronic kidney disease. Although darbepoetin alfa can effectively increase hemoglobin levels, its effect on clinical outcomes in these patients has not been adequately te

  11. Interferon alfa in the treatment paradigm for non-muscle-invasive bladder cancer

    NARCIS (Netherlands)

    Lamm, D.; Brausi, M.; O'Donnell, M.A.; Witjes, J.A.

    2014-01-01

    OBJECTIVES: In this article, we review the various options for and the potential role of interferon alfa (IFN-alpha) in the treatment of non-muscle-invasive bladder cancer (NMIBC). METHODS: PubMed was searched for journal articles on IFN-alpha use in treating bladder cancer. The references listed in

  12. Peginterferon alfa-2a is associated with elevations in alanine aminotransferase at the end of treatment in chronic hepatitis C patients with sustained virologic response.

    Directory of Open Access Journals (Sweden)

    Chih-Wei Tseng

    Full Text Available The purpose of this study was to investigate the incidence and demographic/clinical factors of alanine aminotransferase (ALT abnormalities at the end of treatment (EOT in chronic hepatitis C (CHC patients with sustained virologic response (SVR.Seven hundred naïve CHC patients who underwent combination treatment between January 2003 and December 2010 were included in the study. The patients with SVR and serum ALT>upper limit of normal (ULN at the EOT were further analyzed. The effects of clinical characteristics, treatment regimen, and virologic variables were evaluated by logistic regression. Of the 700 included patients, 488 (69.7% achieved an SVR after treatment, and 235 (33.6% had serum ALT levels>ULN at the EOT. Of those 488 patients, 137 (28.1% had abnormal ALT values at the EOT. A multivariate analysis showed that the occurrence of ALT abnormalities at the EOT was significantly associated with pegylated interferon (PEG-IFN alfa-2a (odds ratio [OR], 2.24; 95% confidence interval [CI], 1.45-3.45; P<0.001, baseline fatty liver (OR, 1.76; 95% CI, 1.16-2.76; P = 0.007, and baseline liver cirrhosis (OR, 2.35; 95% CI, 1.35-4.09; P = 0.002.Use of PEG-IFN-alfa-2a, fatty liver, and cirrhosis are important factors associated with EOT-ALT abnormality in CHC patients receiving combination therapy that achieve an SVR. PEG-IFN-alfa-2a-related EOT-ALT elevation will become normal at the end of follow-up, but fatty liver and cirrhosis-related ALT elevation will not be resolved.

  13. Effects of alfa-hydroxy-isocaproic acid on body composition, DOMS and performance in athletes

    Directory of Open Access Journals (Sweden)

    Karila Tuomo AM

    2010-01-01

    Full Text Available Abstract Background Alfa-Hydroxy-isocaproic acid (HICA is an end product of leucine metabolism in human tissues such as muscle and connective tissue. According to the clinical and experimental studies, HICA can be considered as an anti-catabolic substance. The present study investigated the effects of HICA supplementation on body composition, delayed onset of muscle soreness (DOMS and physical performance of athletes during a training period. Methods Fifteen healthy male soccer players (age 22.1+/-3.9 yr volunteered for the 4-week double-blind study during an intensive training period. The subjects in the group HICA (n = 8 received 583 mg of sodium salt of HICA (corresponding 500 mg of HICA mixed with liquid three times a day for 4 weeks, and those in the group PLACEBO (n = 7 received 650 mg of maltodextrin mixed with liquid three times a day for the same period. According to a weekly training schedule, they practiced soccer 3 - 4 times a week, had strength training 1 - 2 times a week, and had one soccer game during the study. The subjects were required to keep diaries on training, nutrition, and symptoms of DOMS. Body composition was evaluated with a dual-energy X-ray absorptiometry (DXA before and after the 4-week period. Muscle strength and running velocity were measured with field tests. Results As compared to placebo, the HICA supplementation increased significantly body weight (p th week of the treatment (p Conclusion Already a 4-week HICA supplementation of 1.5 g a day leads to small increases in muscle mass during an intensive training period in soccer athletes.

  14. O papel do Fator de Necrose Tumoral Alfa (TNF-alfa no processo de erosão óssea presente no colesteatoma adquirido da orelha média The role of Tumor Necrosis Factor -Alpha (TNF- alpha in bone resorption present in middle ear cholesteatoma

    Directory of Open Access Journals (Sweden)

    Rodrigo Faller Vitale

    2007-02-01

    Full Text Available O colesteatoma adquirido da orelha média causa erosão óssea, com altas taxas de morbidade e mortalidade. O TNF-alfa (TNF-alfa lambda uma das principais citocinas envolvidas neste processo. OBJETIVO: Avaliar o papel do TNF-alfa na reabsorsão óssea e a ação dele no colesteatoma. MATERIAL E MÉTODOS: Foi realizado um levantamento e uma revisão crítica da literatura. RESULTADOS: Todos os autores estudados concordam com a importância do TNF-alfa no processo de reabsorção óssea presente no colesteatoma e com o grau de destruição observado. Diferentes trabalhos demonstraram que o TNF-alfa é capaz de provocar erosão óssea, através de diferentes vias de ação. Ele pode estimular a diferenciação e a maturação dos osteoclastos ou, ainda, agir na matriz óssea expondo-a à ação dos osteoclastos. Existe a possibilidade de inibir a ação do TNF-alfa, diminuindo seus efeitos e prevenindo a perda óssea em doenças como a artrite reumatóide. Não existe, entretanto, trabalhos específicos em colesteatoma. Não existe consenso sobre a sua localização. Estas diferenças, provavelmente, ocorrem devido à distribuição dos receptores. CONCLUSÃO: O TNF-alfa, presente no colesteatoma promove a reabsorsão óssea, juntamente com outras citocinas (RANKL e IL-1, estando relacionado com a presença de complicações.Cholesteatoma may cause bone erosion, with high morbidity and mortality rates. Tumor Necrosis Factor -Alpha (TNF-a is one of the main cytokines involved in this process. Our goal was to evaluate the role of TNF-a in Bone Resorption and its effect on cholesteatoma. MATERIAL AND METHODS: analysis and critical literature review. RESULTS: Different studies have demonstrated that TNF-a is capable of causing bone erosion. It may stimulate the differentiation and maturation of osteoclasts or it may act on the bone matrix, exposing it to the action of the osteoclasts. It is possible to inhibit TNF-a, reducing its effects and prevent

  15. Sustained virological response to treatment of chronic hepatitis C with peginterferon alfa and ribavirin

    Directory of Open Access Journals (Sweden)

    Junior André da Rosa

    2012-06-01

    Full Text Available This study aimed to evaluate the rate of sustained virological response (SVR and the clinical and treatment characteristics of patients with chronic hepatitis C (CHC. A retrospective uncontrolled cohort study was conducted among patients who received treatment for CHC between 2005 and 2008 attended at the Center for the Application and Monitoring of Injectable Medications, in Florianopolis, SC, Brazil. The inclusion criteria were: patients over 18 years of age, with a confirmed diagnosis of chronic hepatitis C according to Brazilian guidelines, treated with PEG-IFN alfa-2a or 2b associated with RBV. A total of 188 patients were included in the study: 70% men, 59% genotype 1, 27% coinfected with HIV, 31% with cirrhosis. The SVR rate, calculated by probability theory, was determined as 26% (max=57.4% and min=12.8% and the intention to treat was 12.8%. Associations between Sustained Virological Response (SVR and the variables sex (p=0.017, age (p=0.003, genotype (p=0.648 and cirrhosis (p=0.275, were determined in the bivariate analysis and only sex and age were significantly associated with SVR. The SVR rate was considered low, which can be partially explained by patients' unfavorable pretreatment characteristics.O objetivo do estudo foi avaliar a taxa de resposta viral sustentada (RVS e as características clínicas e do tratamento dos pacientes portadores de hepatite C crônica. Realizou-se uma coorte retrospectiva não controlada com recorte temporal dos anos de 2005 a 2008, dos pacientes atendidos no Polo de Aplicação e Monitoramento de Medicamentos Injetáveis, em Florianópolis, SC. Os critérios de inclusão foram: pacientes maiores de 18 anos, com diagnóstico confirmado de hepatite C crônica de acordo com o protocolo brasileiro, tratados com PEG-IFN alfa-2a ou 2b associado a ribavirina. Total de 188 pacientes foi incluído no estudo, 70% homens, 59% genótipo 1, 27% co-infectados com o HIV e 31% apresentando cirrose. A taxa de RVS

  16. Safety and tolerance of recombinant interferon alfa-2a (Roferon-A) in cancer patients.

    Science.gov (United States)

    Jones, G J; Itri, L M

    1986-04-15

    Recombinant interferon alfa-2a (Roferon-A, Hoffmann-La Roche Inc., Nutley, NJ) has been evaluated in clinical trials of more than 1300 patients with a broad spectrum of oncologic disease. Patients with either solid tumors or hematologic malignancies were treated with daily or three-times-weekly intramuscular injections for induction periods ranging from 8 to 16 weeks. Doses ranged from 1 X 10(6) units to 124 X 10(6) units per injection. When administered in low daily doses (approximately 3 X 10(6) units), Roferon-A was well tolerated, and dose attenuation was rarely required. Change to three-times-weekly treatment regimen at the same dose was usually sufficient to control toxicity when it occurred in this group of low-dose patients. Those patients receiving higher doses frequently required dose attenuation to 50% of the starting dose to improve clinical tolerance. Virtually all patients treated with Roferon-A experienced some degree of acute toxicity manifested as fever, chills, myalgia, and/or headache. These reactions usually occurred with initial dosing and frequently improved spontaneously with continued administration of the drug. Acetaminophen pretreatment was generally useful in ameliorating these symptoms. Common adverse experiences occurring after repeated dosing included fatigue, anorexia, and weight loss. Serious adverse reactions including cardiovascular and neurologic toxicity have occurred infrequently, primarily at higher doses. Hematologic toxicity and elevations in liver function parameters were also observed, but rarely required dose attenuation. Adverse effects were usually reversible after dose reduction or discontinuation of therapy. Approximately 27% of all patients developed antibodies to rHuIFN-alpha 2A during treatment. No adverse clinical sequelae have been associated with antibody development to date. PMID:3948143

  17. Stroke in patients with type 2 diabetes mellitus, chronic kidney disease, and anemia treated with Darbepoetin Alfa

    DEFF Research Database (Denmark)

    Skali, Hicham; Parving, Hans-Henrik; Parfrey, Patrick S;

    2011-01-01

    More strokes were observed in the Trial to Reduce Cardiovascular Events With Aranesp Therapy (TREAT) among patients assigned to darbepoetin alfa. We sought to identify baseline characteristics and postrandomization factors that might explain this association....

  18. Benefit of Treatment Individualization in Patients with Chronic Hepatitis C Receiving Peginterferon Alfa-2a and Ribavirin in a Large Noninterventional Cohort Study

    Science.gov (United States)

    Hofmann, Wolf Peter; Mauss, Stefan; Lutz, Thomas; Schober, Andreas; Böker, Klaus; Moog, Gero; Baumgarten, Axel; Pfeiffer-Vornkahl, Heike; Alshuth, Ulrich; Hüppe, Dietrich; Wedemeyer, Heiner; Manns, Michael P.; Schott, Eckart

    2015-01-01

    Background and Aims Individualization of treatment with peginterferon alfa and ribavirin in patients with chronic hepatitis C showed benefit in controlled trials and was implemented in treatment guidelines to increase response rates and to reduce side effects and costs. However, it is unknown whether individualization was adopted in routine daily practice and whether it translated into improved outcomes. Methods From a large noninterventional cohort study, clinical and virologic response data of 10,262 HCV patients who received peginterferon alfa-2a and ribavirin between 2003-2007 and 2008-2011 were analyzed. To account for treatment individualization, a matched-pair analysis (2,997 matched pairs) was performed. Variation in treatment duration and dosing of ribavirin were analyzed as indicators for individualization. Results Sustained virological response (SVR) rates were similar between 2003-2007 and 2008-2011 (62.0% vs. 63.7%). Patients with comorbidities were more abundant in the later period, (44.3% vs. 57.1%). The subsequent matched-pair analysis demonstrated higher SVR rates in the 2008-2011 period (64.3%) than in the 2003-2007 period (61.2%, p=0.008). More patients received abbreviated or extended treatment regimens in the later than the earlier period as an indicator of treatment individualization. To the same end, ribavirin doses were higher in the later period (12.6 versus 11.6 mg/kg/day). Factors independently associated with SVR included HCV genotype, low baseline viral load, younger age, route of infection, absence of concomitant diseases, lower APRI score, normal gamma-GT, higher ribavirin doses, no substitution for drug abuse, treatment duration, and treatment in the 2008-2011 period. Conclusions Treatment individualization with peginterferon alfa and ribavirin was implemented in daily routine between 2003-2007 and 2008-2011, SVR rates improved in the same period. These findings may be most relevant in resource-limited settings. PMID:26230998

  19. Tratamento clínico do linfangioma com alfa-2a-interferon Treatment of lymphangioma with interferon-alpha-2a

    Directory of Open Access Journals (Sweden)

    Reynaldo J.S.P. de Souza

    2001-04-01

    Full Text Available OBJETIVO: descrever os resultados obtidos com o uso do alfa-2a-interferon no tratamento de pacientes pediátricos com linfangiomas irressecáveis ou refratários a outros tratamentos. MÉTODO: após revisão da literatura sobre a patogenia e os tratamentos disponíveis para os linfangiomas, utilizamos o alfa-2a-interferon na dosagem de 3 milhões de unidades/m2/dia, via subcutânea, para tratamento de pacientes que apresentavam lesões irressecáveis. Para avaliar o grau de resposta e os efeitos colaterais foram realizadas avaliações clínicas e laboratoriais periódicas. RESULTADOS: observamos que os 6 pacientes com linfangiomas irressecáveis que utilizaram alfa-2a-interferon obtiveram uma resposta clínica satisfatória associada a efeitos colaterais mínimos. Cinco destes evoluíram com regressão parcial de suas lesões e 1 com estabilização da doença. CONCLUSÃO: concluímos que o alfa-2a-interferon constitui uma das armas disponíveis para o tratamento dos linfangiomas inoperáveis na infância.OBJECTIVE: to describe the results of the use of alpha-2a-interferon in the treatment of inoperable childhood lymphangiomas refractory to other therapeutic management. METHOD: we reviewed the literature about pathogenic events and the treatments available for lymphangiomas. Based on that, we used alpha-2a-interferon at a dose of 3 million units/m2/day administered subcutaneously to patients with inoperable disease and no response to other treatments. We conducted periodic clinical and laboratory control to evaluate the response and the adverse reactions to alpha-2a-interferon administration. RESULTS: we observed that the 6 patients with inoperable lymphangiomas who used alpha-2a-interferon had a satisfactory clinical response associated with minimum adverse reactions. Of these patients, 5 had partial regression of their lesions, and 1 remained stable. CONCLUSION: alpha-2a-interferon may be one more available treatment for inoperable childhood

  20. Darbepoietin-alfa has comparable erythropoietic stimulatory effects to recombinant erythropoietin whilst preserving the bone marrow microenvironment

    OpenAIRE

    Dewamitta, Sita R; Russell, Megan R.; Nandurkar, Harshal; Walkley, Carl R

    2013-01-01

    Erythropoiesis stimulating agents are widely used for the treatment of anemia. Recently, we reported erythroid expansion with impaired B lymphopoiesis and loss of trabecular bone in C57BL/6 mice following ten days of treatment with low-dose short acting recombinant human erythropoietin. We have assessed erythropoietin against longer-acting darbepoietin-alfa at a comparable erythroid stimulatory dosage regime. Darbepoietin-alfa and erythropoietin induced similar in vivo erythropoietic expansio...

  1. THE AUTHOR’S EXPERIENCE IN USING BEVACIZUMAB PLUS INTERFERON ALFA-2A IN PATIENTS WITH DISSEMINATED RENAL CELL CARCINOMA

    Directory of Open Access Journals (Sweden)

    A. M. Popov

    2010-01-01

    Full Text Available Target therapy is a main approach to treating metastatic renal cell carcinoma. Bevacizumab plus interferon alfa-2A is now standard first-line options for patients with previously untreated, good or intermediate prognosis (using the Memorial Sloan-Kettering Cancer Center criteria. We have presented our experience in using bevacisumab plus interferon alfa-2A in patients with metastatic renal cell carcinoma. Median progression-free survival and overall survival were 10 months and 22 months, respectively.

  2. THE AUTHOR’S EXPERIENCE IN USING BEVACIZUMAB PLUS INTERFERON ALFA-2A IN PATIENTS WITH DISSEMINATED RENAL CELL CARCINOMA

    Directory of Open Access Journals (Sweden)

    A. M. Popov

    2014-08-01

    Full Text Available Target therapy is a main approach to treating metastatic renal cell carcinoma. Bevacizumab plus interferon alfa-2A is now standard first-line options for patients with previously untreated, good or intermediate prognosis (using the Memorial Sloan-Kettering Cancer Center criteria. We have presented our experience in using bevacisumab plus interferon alfa-2A in patients with metastatic renal cell carcinoma. Median progression-free survival and overall survival were 10 months and 22 months, respectively.

  3. Baseline characteristics of patients in the reduction of events with darbepoetin alfa in heart failure trial (RED-HF)

    OpenAIRE

    McMurray, J J V; Anand, I. S.; Diaz, R.; Maggioni, A. P.; O'Connor, C; Pfeffer, M. A.; Solomon, S D; M. Tendera; van Veldhuisen, D J; Albizem, M.; Cheng, S.; Scarlata, D.; Young, J B; Amuchastegui, M.; Belziti, C.

    2013-01-01

    Aims: This report describes the baseline characteristics of patients in the Reduction of Events with Darbepoetin alfa in Heart Failure trial (RED-HF) which is testing the hypothesis that anaemia correction with darbepoetin alfa will reduce the composite endpoint of death from any cause or hospital admission for worsening heart failure, and improve other outcomes. Methods and results: Key demographic, clinical, and laboratory findings, along with baseline treatment, are reported and compar...

  4. Diversidade alfa e beta no cerrado sensu strictu da Chapada Pratinha, Brasil Alfa and Beta diversity in the cerrado sensu stricto

    OpenAIRE

    Maria Cristina Felfili; Jeanine Maria Felfili

    2001-01-01

    O bioma cerrado possui uma das mais ricas floras dentre as savanas mundiais com mais de 6000 espécies, abrange uma vasta extensão territorial, contém as três maiores bacias hidrográficas sul americanas, e se destaca pela elevada biodiversidade. O objetivo deste trabalho, que faz parte do Projeto Biogeografia do Bioma Cerrado, foi analisar as diversidades alfa e beta em comunidades de cerrado sensu stricto. Foram amostrados o Parque Nacional de Brasília, a Estação Ecológica de Águas Emendadas,...

  5. Managing infertility with the follitropin alfa prefilled pen injector - patient considerations.

    Science.gov (United States)

    Bühler, Klaus

    2015-01-01

    Gonadotropin treatment has been used in fertility treatment since the 1930s. First, preparations coming from animals were injected, then, gonadotropins prepared from the pituitary glands of human cadavers. A great step was achieved with the introduction of human menopausal gonadotropin extracted from the urine of postmenopausal women. When cases of Creutzfeld-Jacob disease were recognized after the use of human pituitary-derived hormone injections, urinary gonadotropins were increasingly purified and then produced by the use of recombinant DNA technology. Recombinant gonadotropins were characterized by the extreme high specificity and the nearly 100% purity. This allows for follitropin alfa, the first recombinant-human follicle stimulating hormone (r-hFSH) approved, to be quantified and filled by mass, with a small variance of only ±2% and no more with a bioassay with a variance of 45%. With recombinant preparations, it is also possible to cover the tremendous growing demand for gonadotropins. Ovarian stimulation has become a self-injecting procedure for the patients. Accurate and easy-to-use injection devices which minimize pain, difficulty, and stress are essential for patient compliance. So, two pen injectors adapted from the well-known insulin pen were introduced in fertility treatment, one as a multiple-use device rechargeable with premixed, prefilled cartridges with r-hFSH (follitropin β) and the other a disposable, prefilled drug delivery system with a liquid formulation of follitropin alfa filled by mass. The efficacy in comparison to the quite more cumbersome handling with ampoules and syringes has been proven very quickly. In several studies, it has been shown that patients had a preference to the prefilled follitropin alfa pen due to the faster preparation and were more confident of accurate dosing. The follitropin alfa (filled by mass [FbM]) prefilled pen is a move toward better quality of treatment and also better quality of life for the women within

  6. Extensive psoriasis induced by interferon alfa treatment for chronic hepatitis C

    OpenAIRE

    Taylor, C; Burns, D.; WISELKA, M

    2000-01-01

    A 47 year old man with chronic hepatitis C was treated with interferon alfa, 3 million units three times a week, and developed widespread plaque psoriasis within weeks of starting interferon therapy. There was no previous history of psoriasis. The psoriasis was characterised by extensive nail involvement and plaques at the interferon injection sites. The patient relapsed after a total of 12 months of interferon and was subsequently treated with interferon and tribavirin (ribavirin) with recur...

  7. Efficacy of Alfa EEG wave biofeedback in the management of anxiety

    OpenAIRE

    Pookala Bhat

    2010-01-01

    Background: Biofeedback is a technique in which people are trained to improve their health by learning to control certain internal bodily processes that normally occur involuntarily. Various studies in the past have shown usefulness of Alfa electroencephalographic (EEG) biofeedback in the alleviation of anxiety symptoms. Though most of the psychiatric centers in the armed forces have this facility, not much work has been done in our setup to assess its efficacy in the management of anxiety. H...

  8. The software and hardware for the ground testing of ALFA- ELECTRON space spectrometer

    Science.gov (United States)

    Batischev, A. G.; Galper, A. M.; Naumov, P. Yu; Naumov, P. P.; Solodovnikov, A. A.

    2016-02-01

    The complex for ground testing and space detector system calibration has been designed. The fast multilayer scintillation detector of the new telescope-spectrometer for the ALFA-ELECTRON space experiment is in ground testing mode now. The device will planned to install on the outer surface of the Russian Segment of the International Space Station. The basic scheme for the detector amplitude parameters measurement by use of specially designed hardware and software are described and the first prototype testing results are demonstrated.

  9. Efficacy of Alfa EEG wave biofeedback in the management of anxiety

    Directory of Open Access Journals (Sweden)

    Pookala Bhat

    2010-01-01

    Full Text Available Background: Biofeedback is a technique in which people are trained to improve their health by learning to control certain internal bodily processes that normally occur involuntarily. Various studies in the past have shown usefulness of Alfa electroencephalographic (EEG biofeedback in the alleviation of anxiety symptoms. Though most of the psychiatric centers in the armed forces have this facility, not much work has been done in our setup to assess its efficacy in the management of anxiety. Hence this study was undertaken. Materials and Methods: This study was carried out in a multispecialty Command Hospital by enrolling 100 patients with psychiatric diagnosis from both inpatient and outpatient services. The anxiety level was assessed clinically and by using Hamilton Anxiety Scale and Taylor′s Manifest Anxiety Scale. One group of 50 patients was treated with Alfa EEG biofeedback sessions only, 5 times in a week for 8 weeks, along with specific pharmacotherapy. The other group was treated with appropriate dose of anxiolytics. The anxiety level was reassessed after 4 weeks and 8 weeks. Results: The response was better for mixed anxiety and depressive disorder with pharmacotherapy than with the biofeedback, but female patients showed better response with EEG biofeedback. Conclusion: In the short term, Alfa EEG biofeedback therapy is almost as efficacious as pharmacological intervention in the management of anxiety symptoms, and relatively more useful in females.

  10. Preparation And Properties Of Bionanocomposite Films Reinforced With Nanocellulose Isolated From Moroccan Alfa Fibres

    Directory of Open Access Journals (Sweden)

    Youssef Benyoussif

    2015-09-01

    Full Text Available Nanocellulose (NC were extracted from the Moroccan Alfa plant (Stipa tenacissima L. and characterised. These Alfa cellulosic nanoparticles were used as reinforcing phase to prepare bionanocomposite films using carboxymethyl cellulose as matrix. These films were obtained by the casting/evaporation method. The crystallinity of NC was analysed by X-ray diffraction, the dimension of NC by atomic force microscopy, molecular interactions due to incorporation of NC in carboxymethyl cellulose (CMC matrix were supported by Fourier transforms infrared (FTIR spectroscopy. The properties of the ensuing bionanocomposite films were investigated using tensile tests, water vapour permeability (WVP study and thermogravimetric analysis. With the progress of purification treatment of cellulose, the crystallinity is improved compared to the untreated fibres; this can be explained by the disappearance of the amorphous areas in cellulose chain of the plant. Consequently, the tensile modulus and tensile strength of CMC film increased by 60 and 47%, respectively, in the bionanocomposite films with 10 wt% of NC, and decrease by 8.6% for WVP with the same content of NC. The NC obtained from the Moroccan Alfa fibres can be used as a reinforcing agent for the preparation of bionanocomposites, and they have a high potential for the development of completely biodegradable food packaging materials.

  11. 重组人干扰素α-1b治疗慢性乙型肝炎导致白癜风1例%A case report of concomitant vitiligo in a patient treated with interferon alfa-1b for chronic hepatitis B infection

    Institute of Scientific and Technical Information of China (English)

    胡明芬; 李云丽; 庄林

    2010-01-01

    @@ 1.病历资料:患者男,12岁,汉族,因体检时发现HBsAg阳性,肝功能异常1个月,于2008年12月1日入院.患者有慢性乙型肝炎家族史.体温36.4℃,脉搏82次/min,呼吸19次/min,血压100/75 mm Hg(1 mmHg=0.133 kPa),皮肤、巩膜无黄染,未见肝掌、蜘蛛痣,皮肤黏膜未见异常,双肺及心脏正常,腹部平软,无压痛,肝脾未触及,肝区有叩击痛,移动性浊音阴性,双下肢无水肿,生理反射正常.入院时肝功能检查ALT 74.0 U/L,AST 60.0 U/L,总胆红素8.3 μmol/L,白蛋白44.1 g/L,球蛋白30.1 g/L.

  12. A randomized controlled clinical study of lower-dose peginterferon alfa-2b in combination with ribavirin in the treatment of chronic hepatitis C

    Institute of Scientific and Technical Information of China (English)

    罗端德

    2006-01-01

    Objective To compare the efficacy and safety of lower-dose peginterferon alfa-2b plus ribavirin versus standard interferon alfa-2b plus ribavirin for treatment of chronic hepatitis C in China. Methods 192 patients with chronic hepatitis C were assigned peginterferon alfa-2b 0.5μg/kg each week plus ribavirin 750~1050 mg/d or standard interferon alfa-2b 3 MIU TIW plus ribavirin 750~1050 mg/d for 48-week treatment and 24-week fellow

  13. Psychiatric adverse effects induced by recombinant interferon alfa in patients with chronic hepatitis C

    Directory of Open Access Journals (Sweden)

    Nešić Zorica I.

    2004-01-01

    Full Text Available Introduction Hepatitis C virus infection is a slowly progressive chronic disease and the most common cause of chronic liver disease. Presently, interferon alfa based therapies, with or without ribavirin, are standard treatment for chronic hepatitis C virus infection. The most troublesome psychiatric side effects of interferon therapy in our patients are: insomnia, irritability, anxiety, mood changes, short temper, emotional and affective lability, impaired cognitive function, apathy, loss of motivation and the most serious depression with or without suicidal ideas. Material and methods In our study we treated 82 patients chronically infected with HCV divided into 3 groups: the first group of 31 patients (20 male and 11 female received IFN-alfa in standard doses of 3 MU three times a week (t.i.w during 24 weeks; the second group of 36 patients (25 male and 11 female received IFN-alfa, 3 MU t.i.w plus ribavirin 1000-1200 mg per day during 24 weeks; the third group of 15 patients (11 male and 4 female received IFN-alfa, 3 MU t.i.w plus ribavirin 1000-1200 mg per day during 48 weeks. The follow-up period after therapy in all groups lasted 24 weeks. Results During treatment, we observed at least one psychiatric side effect in 21 (26% patients: insomnia in 11 (13%, emotional and affective lability in 9 (11%, anxiety, irritability and short temper in 8 (10%, impaired cognitive function in 7 (8% apathia and loss of motivation in 6 (7% treated patients. Depression, the most serious side effect, was established in 8 (10% patients. All of these side effects were observed during later stages of treatment (between 5th and 22nd weeks of treatment. The incidence of all psychiatric side effects was significantly higher in women than in men (p < 0,01. We observed higher prevalence of depression among patients with history of alcohol and drug abuse. Treatment was temporarily discontinued (from 2 to 4 weeks in all patients with depression, but it was not

  14. Peginterferon Alfa-2a plus Ribavirin in Japanese Patients Infected with Hepatitis C Virus Genotype 2 Who Failed Previous Interferon Therapy

    Directory of Open Access Journals (Sweden)

    Tatsuo Kanda, Shingo Nakamoto, Takayoshi Nishino, Nobuo Takada, Akihito Tsubota, Keizo Kato, Tatsuo Miyamura, Daisuke Maruoka, Shuang Wu, Takeshi Tanaka, Makoto Arai, Shigeru Mikami, Keiichi Fujiwara, Fumio Imazeki, Osamu Yokosuka

    2013-01-01

    Full Text Available Some patients infected with hepatitis C virus (HCV genotype 2 could be cured with treatment shorter than 24 weeks using peginterferon plus ribavirin, but there are still treatment-refractory patients. Direct-acting antivirals (DAAs are not currently available for HCV genotype 2 patients, different from genotype 1 patients, in clinical practice. We investigated 29 HCV genotype 2-infected Japanese patients who had been previously treated and failed to clear HCV. We retreated them with peginterferon alfa-2a plus ribavirin and measured HCV RNA level to assess the efficacy and safety of this treatment in patients who had failed previous therapy. We found that retreatment of HCV genotype 2-infected Japanese patients with peginterferon alfa-2a plus ribavirin for 24-48 weeks led to 60 to 66.6% sustained virological response (SVR in patients previously treated with (peg-interferon monotherapy and to 69.9% SVR in relapsers previously treated with peginterferon plus ribavirin. Attention should be paid to certain patients with unique features. Selection of patients according to their previous treatment could lead to optimal therapy in HCV genotype 2 treatment-experienced patients.

  15. Arecibo pulsar survey using ALFA. III. Precursor survey and population synthesis

    Energy Technology Data Exchange (ETDEWEB)

    Swiggum, J. K.; Lorimer, D. R.; McLaughlin, M. A.; Bates, S. D.; Senty, T. R. [Department of Physics and Astronomy, West Virginia University, Morgantown, WV 26506 (United States); Champion, D. J.; Lazarus, P. [Max-Planck-Institut für Radioastronomie, D-53121 Bonn (Germany); Ransom, S. M. [NRAO, Charlottesville, VA 22903 (United States); Brazier, A.; Chatterjee, S.; Cordes, J. M. [Astronomy Department, Cornell University, Ithaca, NY 14853 (United States); Hessels, J. W. T. [ASTRON, Netherlands Institute for Radio Astronomy, Postbus 2, 7990 AA, Dwingeloo (Netherlands); Nice, D. J. [Department of Physics, Lafayette College, Easton, PA 18042 (United States); Ellis, J.; Allen, B. [Physics Department, University of Wisconsin-Milwaukee, Milwaukee WI 53211 (United States); Bhat, N. D. R. [Center for Astrophysics and Supercomputing, Swinburne University, Hawthorn, Victoria 3122 (Australia); Bogdanov, S.; Camilo, F. [Columbia Astrophysics Laboratory, Columbia University, New York, NY 10027 (United States); Crawford, F. [Department of Physics and Astronomy, Franklin and Marshall College, Lancaster, PA 17604-3003 (United States); Deneva, J. S. [Arecibo Observatory, HC3 Box 53995, Arecibo, PR 00612 (United States); and others

    2014-06-01

    The Pulsar Arecibo L-band Feed Array (PALFA) Survey uses the ALFA 7-beam receiver to search both inner and outer Galactic sectors visible from Arecibo (32° ≲ ℓ ≲ 77° and 168° ≲ ℓ ≲ 214°) close to the Galactic plane (|b| ≲ 5°) for pulsars. The PALFA survey is sensitive to sources fainter and more distant than have previously been seen because of Arecibo's unrivaled sensitivity. In this paper we detail a precursor survey of this region with PALFA, which observed a subset of the full region (slightly more restrictive in ℓ and |b| ≲ 1°) and detected 45 pulsars. Detections included 1 known millisecond pulsar and 11 previously unknown, long-period pulsars. In the surveyed part of the sky that overlaps with the Parkes Multibeam Pulsar Survey (36° ≲ ℓ ≲ 50°), PALFA is probing deeper than the Parkes survey, with four discoveries in this region. For both Galactic millisecond and normal pulsar populations, we compare the survey's detections with simulations to model these populations and, in particular, to estimate the number of observable pulsars in the Galaxy. We place 95% confidence intervals of 82,000 to 143,000 on the number of detectable normal pulsars and 9000 to 100,000 on the number of detectable millisecond pulsars in the Galactic disk. These are consistent with previous estimates. Given the most likely population size in each case (107,000 and 15,000 for normal and millisecond pulsars, respectively), we extend survey detection simulations to predict that, when complete, the full PALFA survey should have detected 1000{sub −230}{sup +330} normal pulsars and 30{sub −20}{sup +200} millisecond pulsars. Identical estimation techniques predict that 490{sub −115}{sup +160} normal pulsars and 12{sub −5}{sup +70} millisecond pulsars would be detected by the beginning of 2014; at the time, the PALFA survey had detected 283 normal pulsars and 31 millisecond pulsars, respectively. We attribute the deficiency in normal pulsar

  16. THE INFLUENCE OF ASOCIATED SUPPLEMENT OF ALFA AMYLASE AND XYLANASE ON THE RHEOLOGY OF DOUGH CONCEARNING ITS CONSTITOGRAPHICAL PARAMETERS

    Directory of Open Access Journals (Sweden)

    RODICA CHEREJI

    2013-07-01

    Full Text Available In this paper we determined the influence of associated supplement of alfa amylase and xylanase on the rheology of dough concearning its constitographical parameters : maximum pressure (Pr max, (mb and the absorbed water (Wa, %. The analysis on the consistograph were conducted for constant hydration at the consistency of 500 UF. Determinations were made on 4 types of flour and optimal dosages were found for each enzyme, after which we prepared the optimal dosage of the enzymes in the compund for flour F1 and F2 : P1-840000 U. SKB/100 kg flour +8100 U. FXU /100 kg flour, P2-840000 U. SKB/100 kg flour+16200 U. FXU, /100 kg flour , P3-840000 U. SKB/100 kg flour+24300 U. FXU/100 kg flour , and for F3 and F4 thus: P1-280000 U. SKB/100 kg flour +8100 U. FXU /100 kg flour, P2- 280000 U. SKB/100 kg flour+16200 U. FXU/100 kg flour, P3-280000 U. SKB/100 kg flour+24300 U. FXU/100 kg flour. Fungous α-amylase and xylanase were used in these concentrations to establish which one is more apropriate to be added in flour to obtain superior quality of bread: finer texture of the crumb, prolongation of the freashness of the bread, improvind the colour and flavour, emproving the slicing ability.

  17. Efektivitas Penambahan Vitamin E (alfa-Tokoferol dalam Medium Pencucian Sperma dengan Sentrifugasi terhadap Kualitas Spermatozoa Sapi Brahman

    Directory of Open Access Journals (Sweden)

    Dasrul Dasrul

    2012-10-01

    Full Text Available Effect of vitamin E addition (alfa-tokoferol into sperm washing medium by centrifuge on the quality of Brahman cattle spermatozoa ABSTRACT. The aims of study to determine the effectiveness of the addition of vitamin E in the washing medium by centrifugation on sperm quality Brahman cattle. frozen semen of Brahman cattle, divided into 4 treatment groups addition of vitamin E in the washing medium: 0.0gr/100 ml medium (K0, 0.1gr /100 ml medium (K1; 0.2gr/100 ml medium (K2 and 0.3 g / 100 ml medium (K4, each group was repeated 5 times. Examination of motility, viability and integrity of sperm membrane performed according to WHO standards. The data obtained were analyzed with one-way ANOVA and Duncan test. The average percentage of motility, viability and membrane integrity of spermatozoa in the addition of vitamin E were significantly different (P 0.05 compared with the group K0. The addition of vitamin E in the medium on the process of washing spermatozoa Brahman cattle. The addition of vitamin E 0.2gr/100ml better than vitamin E 0.1gr/100ml and 0.3gr/100ml in maintaining the percentage of motility and live spermatozoa Brahman cattle.

  18. Diversidade alfa e beta no cerrado sensu strictu da Chapada Pratinha, Brasil Alfa and Beta diversity in the cerrado sensu stricto

    Directory of Open Access Journals (Sweden)

    Maria Cristina Felfili

    2001-08-01

    Full Text Available O bioma cerrado possui uma das mais ricas floras dentre as savanas mundiais com mais de 6000 espécies, abrange uma vasta extensão territorial, contém as três maiores bacias hidrográficas sul americanas, e se destaca pela elevada biodiversidade. O objetivo deste trabalho, que faz parte do Projeto Biogeografia do Bioma Cerrado, foi analisar as diversidades alfa e beta em comunidades de cerrado sensu stricto. Foram amostrados o Parque Nacional de Brasília, a Estação Ecológica de Águas Emendadas, a Area de Proteção Ambiental (APA Gama-Cabeça de Veado, Silvânia-GO, Paracatu-MG e Patrocínio-MG. A amostragem foi aleatória com 10 parcelas de 20x50m em cada área de estudo. Foram incluídas plantas lenhosas, exceto lianas, que tivessem no mínimo 5cm de diâmetro. Foi calculada a diversidade alfa pelo teste de Shannon & Wienner e o de Simpson. A diversidade beta foi calculada pelo índice de Whittaker que mede a mudança ou taxa de substituição na composição de espécies de um local para outro. Foi efetuada a curva espécie-área para as 60 parcelas amostradas e efetuada a classificação por TWINSPAN. O cerrado sensu stricto da Chapada Pratinha pode ser diferenciado em duas zonas fitogeográficas: Distrito Federal-Silvânia e Paracatu-Patrocínio. Estas coincidem com um zoneamento por sistemas de terra que classifica a primeira zona como terras altas em contraposição à segunda.The cerrado flora is one of the richest among the world's savannas with more than 6000 species. The cerrado covers a large territory, contains the three most important hydrographycal basins in South America and a high diversity. The objective of this work, that is part of the project Biogeography of the Cerrado Biome, was to analyse alfa and beta diversity in the cerrado sensu stricto. The sampled places were: Brasília National Park -- DF, Águas Emendadas Ecological Station -- DF, Environmentally protected Area of Gama-Cabeça de Veado -- DF, Silv

  19. Interferón alfa-2b tópico como primera opción en las neoplasias intraepiteliales corneoconjuntivales Topical interferon alfa-2b for primary treatment of conjunctiva-cornea intraepithelial neoplasia

    Directory of Open Access Journals (Sweden)

    M. Pérez de Arcelus

    2012-04-01

    Full Text Available Se describen dos casos de neoplasia intraepitlelial corneo-conjuntival (CIN tratados con interferón alfa-2b (IFN alfa-2b tópico como primera elección. El tratamiento clásico de los CIN ha sido tradicionalmente la resección completa con márgenes de seguridad seguida de crioterapia en el lecho quirúrgico. No obstante, y puesto que la tasa de recidivas puede alcanzar el 50% han sido propuestos coadyuvantes como la mitomicina C y el 5 fluoracilo, con el consiguiente riesgo de toxicidad corneal y límbica. El IFN alfa-2b presenta una eficacia similar a la cirugía en la erradicación completa de la masa tumoral como primera opción, con escasos efectos secundarios y nulo potencial carcinogénico, incluso en casos de recurrencia a terapia con mitomicina C, lesiones quirúrgicas residuales y formas difusas.We describe two cases of conjunctival-cornea intraepithelial neoplasia (CIN, treated with topical IFN alfa 2b. The traditional treatment for CIN is surgical excision usually with adjunctive cryotherapy. However, residual tumour may remain, which can lead to recurrence rates of more than 50%. 5-Fluorouracil, mitomicyn C and interferon alfa 2b are new pharmacological agents that have proved their efficacy in the treatment of CIN. As side effects are common, we present IFN alfa 2b as a single therapeutic agent as an effective and optimal treatment for presumed recurrent corneal and conjunctival intraepithelial neoplasia. It offers the benefits of topical therapy and avoids the risks of surgical or other interventions - specifically, ocular surface toxicity, cicatricial conjunctival changes, and limbal stem cell deficiency.

  20. The Arecibo Legacy Fast ALFA Survey: X. The HI Mass Function and Omega_HI From the 40% ALFALFA Survey

    CERN Document Server

    Martin, Ann M; Giovanelli, Riccardo; Haynes, Martha P; Springob, Christopher M; Stierwalt, Sabrina

    2010-01-01

    The Arecibo Legacy Fast ALFA (ALFALFA) survey has completed source extraction for 40% of its total sky area, resulting in the largest sample of HI-selected galaxies to date. We measure the HI mass function from a sample of 10,119 galaxies with 6.2 < log (M_HI/M_Sun) < 11.0 and with well-described mass errors that accurately reflect our knowledge of low-mass systems. We characterize the survey sensitivity and its dependence on profile velocity width, the effect of large-scale structure, and the impact of radio frequency interference in order to calculate the HIMF with both the 1/Vmax and 2DSWML methods. We also assess a flux-limited sample to test the robustness of the methods applied to the full sample. These measurements are in excellent agreement with one another; the derived Schechter function parameters are phi* = 4.8 (+/- 0.3) * 10^-3, log (M*/M_Sun) + 2 log(h_70) = 9.96 (+/- 0.2), and alpha = -1.33 (+/- 0.02). We find Omega_HI = 4.3 (+/- 0.3) * 10^-4, 16% larger than the 2005 HIPASS result, and ou...

  1. Adjuvant therapy with pegylated interferon alfa-2b versus observation alone in resected stage III melanoma: final results of EORTC 18991, a randomised phase III trial.

    NARCIS (Netherlands)

    Eggermont, A.M.; Suciu, S.; Santinami, M.; Testori, A.; Kruit, W.H.; Marsden, J.; Punt, C.J.A.; Sales, F.; Gore, M.; Mackie, R.; Kusic, Z.; Dummer, R.; Hauschild, A.; Musat, E.; Spatz, A.; Keilholz, U.

    2008-01-01

    BACKGROUND: Any benefit of adjuvant interferon alfa-2b for melanoma could depend on dose and duration of treatment. Our aim was to determine whether pegylated interferon alfa-2b can facilitate prolonged exposure while maintaining tolerability. METHODS: 1256 patients with resected stage III melanoma

  2. Changes of T Lymphocyte Subsets and Th1,Th2 Cytokines in Patients with Chronic Hepatitis C before and after Peg-Interferon Alfa-2b Therapy%聚乙二醇干扰素治疗丙型肝炎前后淋巴细胞亚群及辅助性T淋巴细胞因子的变化

    Institute of Scientific and Technical Information of China (English)

    胡敏涛; 毛燕群; 裴豪; 汪铮

    2011-01-01

    目的 研究聚乙二醇干扰素α-2b(PEG-IFNα-2b)治疗慢性丙型肝炎(丙肝)的疗效与机体免疫功能的关系.方法 采用流式细胞仪检测PEG-IFNα-2b治疗前后丙肝患者T淋巴细胞亚群[CD3+、CD4+、CD8+T细胞]及CD4+/CD8+比值、自然杀伤(NK)细胞、细胞毒性T细胞(CTL)的变化;用双抗体夹心酶联免疫吸附试验检测治疗前后患者血清干扰素γ(IFN-γ)、IL-2、IL-6、IL-10的变化.结果 应答组患者治疗前后CD3+、CD4+、CD8+、NK细胞、CTL及CD4+/CD8+比较,差异均有统计学意义(P<0.01);治疗48周后应答组与无应答组比较,CD3+、CD4+、NK细胞、CD4+/CD8+及IFN-γ、IL-2水平均显著升高,而CD8+、CTL及IL-6、IL-10水平则显著降低,差异均有统计学意义(P<0.05).结论 PEG-IFNα-2b治疗后患者的细胞免疫功能明显增强,通过检测患者T淋巴细胞亚群、NK细胞、CTL及血清IFN-γ、IL-2、IL-6、IL-10的变化,可以预测患者干扰素治疗的近期疗效.%Objective To study the relationship between the immune state and the efficacy of peg - interferon alfa - 2b ( PEG -IFNa-2b ) of patients with hepatitis C. Methods Using flow cytometry, we studied the changes of T lymphocyte subtypes ( CD3 + , CD4 + , CD8 + ), CD4 +/CD8 + ratio, NK, and CTL in patients with chronic hepatitis C before and after PEG -IFNa - 2b therapy. Using double antibody sandwich ELBA, we studied the changes of serum IFN-γ, IL-2, IL- 6, IL-10 levels in patients with chronic hepatitis C before and after PEG - IFNa - 2b therapy. Results T lymphocyte subtypes ( CD3 +, CD4 + , CD8 + ), CD4 +/CD8 + ratio, NK, and CTL were significantly different before and after treatment in the responder group ( P<0. 01 ). After 48 weeks of treatment, levels of CD3 + cells, CD4 + cells, NK cells, and IFN-7, IL - 2 increased, while CD8 + cells, CTL cells and IL - 6, IL - 10 decreased in the responder group in comparison with the non - responder group ( P < 0. 05 ) . Conclusion The cellular

  3. Interferón alfa-2b tópico como primera opción en las neoplasias intraepiteliales corneoconjuntivales Topical interferon alfa-2b for primary treatment of conjunctiva-cornea intraepithelial neoplasia

    OpenAIRE

    M. Pérez de Arcelus; M Aranguren; J. Andonegui

    2012-01-01

    Se describen dos casos de neoplasia intraepitlelial corneo-conjuntival (CIN) tratados con interferón alfa-2b (IFN alfa-2b) tópico como primera elección. El tratamiento clásico de los CIN ha sido tradicionalmente la resección completa con márgenes de seguridad seguida de crioterapia en el lecho quirúrgico. No obstante, y puesto que la tasa de recidivas puede alcanzar el 50% han sido propuestos coadyuvantes como la mitomicina C y el 5 fluoracilo, con el consiguiente riesgo de toxicidad corneal ...

  4. GSI Helmholz Centre for Heavy Ion Research - ALFA: Next generation concurrent framework for ALICE and FAIR experiments

    CERN Document Server

    CERN. Geneva

    2015-01-01

    FAIR is a new, unique international accelerator facility for the research with antiprotons and ions. It is being built at the GSI Darmstadt in Hesse, Germany. The commonalities between the ALICE and FAIR experiments and their computing requirements led to the development of a common software framework in an experiment independent way; ALFA (ALICE-FAIR framework). ALFA is designed for high quality parallel data processing and reconstruction on heterogeneous computing systems. It provides a data transport layer and the capability to coordinate multiple data processing components. ALFA is a flexible, elastic system which balances reliability and ease of development with performance by using a message based multi-processing in addition to multi-threading. The framework allows for usage of heterogeneous computing architectures by offloading (portions of code are accelerated on the device) or natively (where the full program is executed on the device ).

  5. Mapping Hydrogen in the Galaxy, Galactic Halo, and Local Group with ALFA: The GALFA-HI Survey Starting with TOGS

    CERN Document Server

    Gibson, S J; Heiles, C; Korpela, E J; Peek, J E G; Putman, M E; Stanimirović, S

    2008-01-01

    Radio observations of gas in the Milky Way and Local Group are vital for understanding how galaxies function as systems. The unique sensitivity of Arecibo's 305m dish, coupled with the 7-beam Arecibo L-Band Feed Array (ALFA), provides an unparalleled tool for investigating the full range of interstellar phenomena traced by the HI 21cm line. The GALFA (Galactic ALFA) HI Survey is mapping the entire Arecibo sky over a velocity range of -700 to +700 km/s with 0.2 km/s velocity channels and an angular resolution of 3.4 arcminutes. We present highlights from the TOGS (Turn on GALFA Survey) portion of GALFA-HI, which is covering thousands of square degrees in commensal drift scan observations with the ALFALFA and AGES extragalactic ALFA surveys. This work is supported in part by the National Astronomy and Ionosphere Center, operated by Cornell University under cooperative agreement with the National Science Foundation.

  6. Unmasking of myasthenia gravis during pegylated Alfa 2 a interferon and ribavirin therapy for chronic hepatitis C.

    Science.gov (United States)

    Saleem, Ayesha

    2016-05-01

    Over last few decades, hepatitis C has emerged as a serious infection that has threatened the health and budgets of millions in the world. The objective of health professionals to treat it with recommended therapy of Alfa interferon and Ribavirin combination presents certain risks. One of the alarms is the ability of interferon to stimulate the production of autoantibodies in the body resulting in expression of autoimmune diseases in few who develop these antibodies. The case presented here is about unmasking of myasthenia gravis in a patient who received alfa interferon therapy for her chronic hepatitis C. Alfa interferon probably plays an important role in manifestation of the diseases in susceptible patients and all autoimmune diseases cannot be taken as mere side effects of the therapy. Clinicians need to be alert to pick up these diseases earlier so that the prompt management is possible. PMID:27183950

  7. Effects of Darbepoetin Alfa with exercise in cancer patients undergoing chemotherapy: an explorative study

    DEFF Research Database (Denmark)

    Rørth, Mikael; Rich Madsen, Katrine; Burmølle, Stine Hvid;

    2011-01-01

    had received at least one cycle chemotherapy, participated. Participants received 500 µg Darbepoetin Alfa (DA) every 3 weeks during the intervention. Four typologies of patients were identified with regard to DA effects. The interviews revealed that eleven patients experienced some kind of immediate...... improvement in cognitive and emotional functioning, and subjective daily well-being. Furthermore physical improvement and changes in QoL outcomes showed no significant differences between the study group and a reference group. A significant increase in the hemoglobin concentration (7.14–7.87 mmol/L, P<0...

  8. Uloga alfa adrenergičkog sistema u regulaciji pulpne cirkulacije

    OpenAIRE

    Ibričević, Hamijeta; Đorđević, Nebojša; Juhas-Pašič, Eva

    1989-01-01

    Pulpna cirkulacija je mikrocirkulacija, čija je primarna funkcija transport nutritivnih i povratak otpadnih metaboličkih produkata. Obzirom da je pulpa okružena tvrdim dentinskim oklopom, od posebne je važnosti za njeno normalno funkcioniranje u fiziološkim prilikama i za preživljavanje u patološkim stanjima, da regulacija cirkulacije kao i nivoi intra- i ekstracelularne tekućine budu dobro regulirani. U toj regulaciji presudnu ulogu ima alfa adrenergički sistem sa svim svojim komponentama....

  9. Incidence and Severity of Acute Allograft Rejection in Liver Transplant Recipients Treated With Alfa lnterferon

    OpenAIRE

    Jain, Ashokkumar; Demetris, Anthony J.; Manez, Rafael; Tsamanadas, Athanassisos C.; Thiel, David; Rakela, Jorge; Starzl, Thomas E.; Fung, John J.

    1998-01-01

    Interferon alfa-2b (IFN-α) therapy has been shown to be effective in the treatment of viral hepatitis B (HBV) or viral hepatitis C (HCV) in patients who did not undergo transplantation. However, in allograft recipients, treatment with IFN-α often leads to allograft rejection. The aim of the present study was to determine if IFN-α therapy increases the incidence or severity of acute rejection in human liver allograft recipients. One hundred five orthotopic liver transplant (OLT) recipients wit...

  10. Managing infertility with the follitropin alfa prefilled pen injector – patient considerations

    Directory of Open Access Journals (Sweden)

    Bühler K

    2015-06-01

    Full Text Available Klaus Bühler Centre for Gynaecological Endocrinology and Reproductive Medicine, Stuttgart, Germany Abstract: Gonadotropin treatment has been used in fertility treatment since the 1930s. First, preparations coming from animals were injected, then, gonadotropins prepared from the pituitary glands of human cadavers. A great step was achieved with the introduction of human menopausal gonadotropin extracted from the urine of postmenopausal women. When cases of Creutzfeld-Jacob disease were recognized after the use of human pituitary-derived hormone injections, urinary gonadotropins were increasingly purified and then produced by the use of recombinant DNA technology. Recombinant gonadotropins were characterized by the extreme high specificity and the nearly 100% purity. This allows for follitropin alfa, the first recombinant-human follicle stimulating hormone (r-hFSH approved, to be quantified and filled by mass, with a small variance of only ±2% and no more with a bioassay with a variance of 45%. With recombinant preparations, it is also possible to cover the tremendous growing demand for gonadotropins. Ovarian stimulation has become a self-injecting procedure for the patients. Accurate and easy-to-use injection devices which minimize pain, difficulty, and stress are essential for patient compliance. So, two pen injectors adapted from the well-known insulin pen were introduced in fertility treatment, one as a multiple-use device rechargeable with premixed, prefilled cartridges with r-hFSH (follitropin β and the other a disposable, prefilled drug delivery system with a liquid formulation of follitropin alfa filled by mass. The efficacy in comparison to the quite more cumbersome handling with ampoules and syringes has been proven very quickly. In several studies, it has been shown that patients had a preference to the prefilled follitropin alfa pen due to the faster preparation and were more confident of accurate dosing. The follitropin alfa

  11. Estudio de una muestra completa de galaxias con emisiones en H"Alfa"

    OpenAIRE

    Gallego Maestro, Jesús

    1995-01-01

    Este trabajo consiste en caracterizar la muestra de galaxias con emisión en h "alfa" obtenida de la exploración UCM. En total 272 objetos hasta un desplazamiento al rojo de 0.045 analizados espectroscópicamente. Cada uno de ellos fue clasificado según ocho tipos espectroscópicos. Principalmente (47%) se trata de galaxias con intensos procesos de formación estelar. Por otro lado, se ha encontrado un 43% de objetos de baja ionización que no se detectan con otras técnicas. Los diferentes tipos o...

  12. Valor diagnóstico de la alfa-fetoproteína en el carcinoma hepatocelular

    OpenAIRE

    Hernández Perera, Julio César

    2010-01-01

    En el mundo el hepatocarcinoma (CHC) es la quinta a sexta neoplasia más frecuente. En Cuba se avizora un incremento de su incidencia como consecuencia del envejecimiento poblacional y el incremento de la morbi-mortalidad por la cirrosis hepática (CH). En este trabajo se empleó la alfa-fetoproteína (AFP) por técnica SUMA como marcador tumoral en 189 pacientes cirróticos evaluados en el CIMEQ, entre enero de 1999 y septiembre de 2005. Los principales factores que se asociaron a una elevación de...

  13. Complex treatment of allergic eye diseases using interferon alfa-2b and loratadine gel for topical and external use

    Directory of Open Access Journals (Sweden)

    A. V. Surov,

    2014-01-01

    Full Text Available Aimis to determine the efficacy of Allergoferon® — combined medicine composed of interferon alfa-2b and loratadine gel for topical and external use — in ocular allergies treatment.Materials and methods. 60 patients with allergic eye diseases were treated. Allergoferon® gel applied QID on upper and lower eyelids was used as a first-line therapy. Efficacy was measured using the following criteria: chemozis, eyelid edema, conjunctival redness and discharge, the presence of conjunctival follicles and papillae. All symptoms were scored using a 4‑point scale (0 = none, 1 = mild, 2 = moderate, 3 = severe.Results. Topical administration of Allergoferon® was associated with significant symptoms reduction, i.e., chemozis by 82 %, eyelid edema by 91 %, conjunctival redness by 79 %, eyelid redness by 88 %, conjunctival discharge by 95 %, the presence of conjunctival follicles and papillae by 76 %.Conclusion. Allergoferon® gel for topical and external use quickly reduces tissue allergic reactions and, consequently, terms of treatment. This medication is highly-effective as a therapy of ocular allergies owing to its antihistamine, anti-inflammatory and anti-exudative effects.

  14. alfa-Deuterium kinetic isotope effects in reactions of methyllithium. Is better aggregation the cause of lower reactivity?

    DEFF Research Database (Denmark)

    Holm, Torkil

    1996-01-01

    The value of kH/kD for alfa deuterium kinetic isotope effects for the reaction of methyllithium and methylmagnesium iodid with a series of substrates are consistently ca. 10-15 % higher for the lithium reagent. This may indicate a pre-equilibrium......The value of kH/kD for alfa deuterium kinetic isotope effects for the reaction of methyllithium and methylmagnesium iodid with a series of substrates are consistently ca. 10-15 % higher for the lithium reagent. This may indicate a pre-equilibrium...

  15. Alfa-glucosidase and chymotrypsin inhibiting lignans from commiphora mukul

    International Nuclear Information System (INIS)

    Phytochemical investigation of the whole plant of Commiphora mukul Engl. resulted in the isolation of two lignans, epiexcelsin (1) and 5 -demethoxyepiexcelsin (2) which are reported for the first time from this species. The structure elucidation of the isolated compounds was based on 1D and 2D-NMR analysis and by the comparison with the published data. The lignans 1 and 2 showed significant inhibitory activity against -glucosidase with the IC/sub 50/ of 59.8 +- 3.63455 micro meter and 75.2 +- 8.1616 micro meter respectively. They also showed weak inhibitory potential against chymotrypsin with the IC/sub 50/ of 110 +- 0.025 micro meter and 649 +- 0.013 micro meter respectively. (author)

  16. Alfa-Galactosidases and their applications in biotransformations

    Czech Academy of Sciences Publication Activity Database

    Weignerová, Lenka; Simerská, Pavla; Křen, Vladimír

    2009-01-01

    Roč. 27, č. 2 (2009), s. 79-89. ISSN 1024-2422 R&D Projects: GA MŠk(CZ) LC06010; GA ČR GA203/05/0172 Institutional research plan: CEZ:AV0Z50200510 Keywords : -Galactosidase * enzyme * transglycosylation Subject RIV: EI - Biotechnology ; Bionics Impact factor: 1.017, year: 2009

  17. Mango resistance to fruit flies. II - resistance of the alfa cultivar

    Energy Technology Data Exchange (ETDEWEB)

    Rossetto, C.J.; Bortoletto, N., E-mail: rossetto@iac.sp.gov.b [Agencia Paulista de Tecnologia dos Agronegocios (APTA), Votuporanga, SP (Brazil). Polo Regional do Noroeste Paulista; Walder, J.M.M.; Mastrangelo, T. de A., E-mail: jmwalder@cena.usp.b [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Carvalho, C.R.L.; Castro, J.V. de, E-mail: climonta@iac.sp.gov.b, E-mail: josalba@iac.sp.gov.b [Instituto Agronomico de Campinas, SP (Brazil); Pinto, A.C. de Q. [EMBRAPA, Brasilia, DF (Brazil); Cortelazzo, A.L., E-mail: angelo@unicamp.b [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Inst. de Biologia

    2006-07-01

    The percentage of infested mango fruits of five selected mango varieties was evaluated during three years under field conditions. Three varieties with field resistance to fruit flies had less then 10% of fruits infested. Tommy Atkins, the susceptible commercial check, had 42,9% and the susceptible check had 98.9 % of infested fruits. The three field resistant varieties plus the susceptible commercial check, Tommy Atkins, were further tested in laboratory, under caged conditions, with artificial infestation of Anastrepha obliqua. The attempts of oviposition and the number of pupae developed from each fruit were evaluated. Under caged conditions, the cultivar Alfa maintained its field resistance and Espada Stahl and IAC 111 lost the field resistance and were as susceptible as Tommy Atkins. The attempts of oviposition were positively and highly correlated with the number of pupae developed in the fruits. Non preference for oviposition was confirmed as the main mechanism of resistance of mango fruits to fruit flies. In the absence of a more susceptible variety (no choice test) the cultivar Alfa has kept the resistance (author)

  18. LCDM Satellites and HI Companions - The Arecibo ALFA Survey of NGC 2903

    CERN Document Server

    Irwin, J A; Spekkens, K; Haynes, M P; Giovanelli, R; Linder, S M; Catinella, B; Momjian, E; Koribalski, B S; Davies, J; Brinks, E; De Blok, W J G; Putman, M E; Van Driel, W

    2008-01-01

    We have conducted a deep, complete HI survey, using Arecibo/ALFA, of a field centered on the nearby, isolated galaxy, NGC 2903, which is similar to the Milky Way in its properties. The field size was 150 kpc x 260 kpc and the final velocity range spanned from 100 to 1133 km/s. The ALFA beams have been mapped as a function of azimuth and cleaned from each azimuth-specific cube prior to forming final cubes. The final HI data are sensitive down to an HI mass of 2 x 10^5 Mo and column density of 2 x 10^{17} cm^{-2} at the 3sigma x 2deltaV level, where sigma is the rms noise level and deltaV is the velocity resolution. NGC 2903 is found to have an HI envelope that is larger than previously known, extending to at least 3 times the optical diameter of the galaxy. Our search for companions yields one new discovery with an HI mass of 2.6 x 10^6 Mo. The companion is 64 kpc from NGC 2903 in projection, is likely associated with a small optical galaxy of similar total stellar mass, and is dark matter dominated, with a to...

  19. Mango resistance to fruit flies. II - resistance of the alfa cultivar

    International Nuclear Information System (INIS)

    The percentage of infested mango fruits of five selected mango varieties was evaluated during three years under field conditions. Three varieties with field resistance to fruit flies had less then 10% of fruits infested. Tommy Atkins, the susceptible commercial check, had 42,9% and the susceptible check had 98.9 % of infested fruits. The three field resistant varieties plus the susceptible commercial check, Tommy Atkins, were further tested in laboratory, under caged conditions, with artificial infestation of Anastrepha obliqua. The attempts of oviposition and the number of pupae developed from each fruit were evaluated. Under caged conditions, the cultivar Alfa maintained its field resistance and Espada Stahl and IAC 111 lost the field resistance and were as susceptible as Tommy Atkins. The attempts of oviposition were positively and highly correlated with the number of pupae developed in the fruits. Non preference for oviposition was confirmed as the main mechanism of resistance of mango fruits to fruit flies. In the absence of a more susceptible variety (no choice test) the cultivar Alfa has kept the resistance (author)

  20. Drotrecogin alfa (activated)...a sad final fizzle to a roller-coaster party.

    Science.gov (United States)

    Angus, Derek C

    2012-01-01

    Following the failure of PROWESS-SHOCK to demonstrate efficacy, Eli Lilly and Company withdrew drotrecogin alfa (activated) from the worldwide market. Drotrecogin was initially approved after the original trial, PROWESS, was stopped early for overwhelming efficacy. These events prompt consideration of both the initial approval decision and the later decision to withdraw. It is regrettable that the initial decision was made largely on a single trial that was stopped early. However, the decision to approve was within the bounds of normal regulatory practice and was made by many approval bodies around the world. Furthermore, the overall withdrawal rate of approved drugs remains very low. The decision to withdraw was a voluntary decision by Eli Lilly and Company and likely reflected key business considerations. Drotrecogin does have important biologic effects, and it is probable that we do not know how best to select patients who would benefit. Overall, there may still be a small advantage to drotrecogin alfa, even used non-selectively, but the costs of determining such an effect with adequate certainty are likely prohibitive, and the point is now moot. In the future, we should consider ways to make clinical trials easier and quicker so that more information can be available in a timely manner when considering regulatory approval. At the same time, more sophisticated selection of patients seems key if we are to most wisely test agents designed to manipulate the septic host response. PMID:22309988

  1. Macroscale production of crystalline interferon alfa-2b in microgravity on STS-52

    Science.gov (United States)

    Nagabhushan, Tattanahalli L.; Reichert, Paul; Long, Marianna M.; DeLucas, Lawrence J.; Bugg, Charles E.

    1995-01-01

    Macroscale crystallization of zinc interferon alfa-2b was achieved on STS-52 in October 1992 in the Protein Crystallization Facility. Conditions for crystallization were established by adapting a microscale vapor diffusion method to a macroscale temperature induction method. A series of earth based pilot experiments established conditions to reproducibly crystallize zinc interferon alfa-2b in high yield and under cleanroom conditions. As a control for the STS-52 mission, a ground experiment was run simultaneously and in the same configuration as the flight experiment. Greater than 95% of the available protein crystallized in both the ground and flight experiments. Using a battery of physical, biochemical and biological characterization assays, demonstrated that sample processing, polysulfone bottle confinement and the conditions used for crystallization did not have a negative effect on protein integrity. Redissolved crystals from the flight and ground experiments showed full biological activity in a cytopathic effect inhibition assay as compared to an interferon control standard. Morphometric analysis comparing the overall length and width of the derived crystals showed a 2.4 fold increase in the length and width of the space grown crystals as compared to earth grown crystals. Space grown crystals have remained a stable free flowing suspension for over 2 years. Based on these results, further experiments are envisioned to investigate macroscale crystallization of biologically active macromolecules in microgravity.

  2. Neuropsychiatric side-effects of interferon alfa therapy for hepatitis C and their management: A review

    Directory of Open Access Journals (Sweden)

    Al-Huthail Yaser

    2006-01-01

    Full Text Available Background:Interferon-Alfa (IFN-α has been the mainstay of treatment for chronic hepatitis C. It has the potential to alter the course of chronic hepatitis C and prevent complications. Aims: This review summarizes current knowledge about the presentation, mechanism, course, and treatment of neuropsychiatric problems associated with interferon alfa (IFN-α treatment. Methods: Studies were identified by computerized searches, and further references were obtained from bibliographies of the reviewed articles. Results: Neuropsychiatric symptoms frequently occur in hepatitis C patients treated with IFNAlfa. These side effects are troublesome and frequently account for dose reduction or treatment discontinuation. Sometimes, they could be serious and possibly life threatening. Frequent psychiatric evaluations of high risk individuals and, when required, early administration of antidepressants could possibly help to avoid the development of an impending depression or reduce its severity and duration and thus increase adherence to treatment. Conclustion: Greater cooperation between psychiatrists and hepatologists would mean less risk of having the serious side effects with more importance needs to be given to the identification of patients at risk.

  3. Interleukin-10 promoter polymorphism predicts initial response of chronic hepatitis B to interferon alfa

    Directory of Open Access Journals (Sweden)

    Ma Weimin

    2011-01-01

    Full Text Available Abstract In order to examine whether variation in interleukin-10 promoter polymorphism would predict the likelihood of sustain response of chronic hepatitis B to treatment with interferon alfa (IFN-α, the inheritance of 3 biallelic polymorphisms in the IL-10 gene promoter in patients with 52 chronic hepatitis B were determined by polymerase chain reaction (PCR-bared techniques, restriction enzyme digestion or direct sequencing. The relationship to the outcome of antiviral therapy for chronic HBV infection was studied in 24 patients who had a virologically sustained response(SR and in 28 non-responder(NR to interferon alfa-2b and several IL-10 variants were more frequent among SR compared with NR. Carriage of the -592A allele, -592A/A genotype and -1082/-1819/-592 ATA haplotype was associated with SR. Our findings indicate that heterogeneity in the promoter region of the IL-10 gene has a role in determining the initial response of chronic hepatitis B to IFN-α therapy.

  4. ARECIBO PULSAR SURVEY USING ALFA: PROBING RADIO PULSAR INTERMITTENCY AND TRANSIENTS

    International Nuclear Information System (INIS)

    We present radio transient search algorithms, results, and statistics from the ongoing Arecibo Pulsar ALFA (PALFA) survey of the Galactic plane. We have discovered seven objects through a search for isolated dispersed pulses. All of these objects are Galactic and have measured periods between 0.4 and 4.7 s. One of the new discoveries has a duty cycle of 0.01%, smaller than that of any other radio pulsar. We discuss the impact of selection effects on the detectability and classification of intermittent sources, and compare the efficiencies of periodicity and single-pulse (SP) searches for various pulsar classes. For some cases we find that the apparent intermittency is likely to be caused by off-axis detection or a short time window that selects only a few bright pulses and favors detection with our SP algorithm. In other cases, the intermittency appears to be intrinsic to the source. No transients were found with DMs large enough to require that they originate from sources outside our Galaxy. Accounting for the on-axis gain of the ALFA system, as well as the low gain but large solid-angle coverage of far-out sidelobes, we use the results of the survey so far to place limits on the amplitudes and event rates of transients of arbitrary origin.

  5. PMF: the front end electronic of the ALFA detector

    CERN Document Server

    Barrillon, P; Cheikali, C; Cuisy, D; Gaspard, M; Fournier, D; Heller, M; Iwanski, W; Lavigne, B; de La Taille, C; Puzo, P; Socha, J-L

    2008-01-01

    The front end electronic (PMF) of the future ATLAS luminometer is described here. It is composed by a MAPMT and a compact stack of three PCBs which deliver the high voltage, route and readout the output signals. The third board contains a FPGA and MAROC, a 64 channels ASIC which can correct the non uniformity of the MAPMT channels gain thanks to a variable gain preamplifier. Its main role is to shape and discriminate the input signals at 1/3 photo-electron and produce 64 trigger outputs. Laboratory tests performed on prototype and pre-series PMFs have showed performances in good agreement with the requirements.

  6. Neuropharmacological effects of alfa-cypermethrin in rats

    Directory of Open Access Journals (Sweden)

    Manna S

    2005-01-01

    Full Text Available OBJECTIVE: To study the effect of α-cypermethrin (α-CP on some neuropharmacological paradigms like motor coordination, pentobarbitone-induced sleeping time and pentylenetetrazole-induced (PTZ convulsion as well as γ-aminobutyric acid (GABA level in rat brain. MATERIALS AND METHODS: Albino Wistar rats were the experimental animals. Different neuropharmacological paradigms like motor coordination (determined by rotarod, pentobarbitone-induced sleeping time and PTZ-induced convulsion were carried out following oral administration of α-CP at two dose levels i.e., 145 mg/kg (LD50 and 14.5 mg/kg (1/10LD50, while the level of GABA in the brain of rats was estimated by HPLC after single-dose oral administration of α-CP at 145 mg/kg. RESULTS: α-CP induced significant motor incoordination, decreased the time of onset and increased the duration of sleeping time induced by pentobarbitone; and also decreased the time of onset and increased the duration of convulsion induced by PTZ at the dose levels of 145 mg/kg (LD50 and 14.5 mg/kg (1/10 LD50 respectively. Further α-CP decreased brain GABA levels in the cerebellum and in whole brain (except cerebellum significantly at LD50 dose level. CONCLUSION: A correlation between the effect of α-CP on central GABA levels and its neuropharmacological effects can be hypothesized.

  7. Interferon alfa-2a versus combination therapy with interferon alfa-2a, interleukin-2, and fluorouracil in patients with untreated metastatic renal cell carcinoma (MRC RE04/EORTC GU 30012): an open-label randomised trial.

    NARCIS (Netherlands)

    Gore, M.E.; Griffin, C.L.; Hancock, B.; Patel, P.M.; Pyle, L.; Aitchison, M.; James, N.; Oliver, R.T.D.; Mardiak, J.; Hussain, T.; Sylvester, R.; Parmar, M.K.; Royston, P.; Mulders, P.F.A.

    2010-01-01

    BACKGROUND: In metastatic renal cell carcinoma combinations of interferon alfa-2a, interleukin-2, and fluorouracil produce higher response rates and longer progression-free survival than do single agents. We aimed to compare overall survival in patients receiving combination treatment or interferon

  8. PMF: the front end electronic of the ALFA detector

    CERN Document Server

    Barrillon, P; Cheikali, C; Cuisy, D; Gaspard, M; Fournier, D; Heller, M; Iwanski, W; Lavigne, B; de La Taille, C; Puzo, P; Socha, J-L

    2010-01-01

    The front end electronic (PMF) of the future ATLAS luminometer is described here. It is composed of a MAPMT and a compact stack of three PCBs, which deliver high voltage, route and read out of the output signals. The third board contains an FPGA and MAROC, a 64-channel ASIC, which can correct the non-uniformity of the MAPMT channels gain, thanks to a variable gain preamplifier. Its main role is to shape and discriminate the input signals at 1/3 photo-electron and produce 64 trigger outputs. Laboratory tests performed on prototype and pre-series PMFs have showed performances in good agreement with the requirements and have fulfilled the approval criteria for the final production of all elements.

  9. PMF: The front end electronic of the ALFA detector

    Energy Technology Data Exchange (ETDEWEB)

    Barrillon, P., E-mail: barrillo@lal.in2p3.f [Laboratoire de l' Accelerateur Lineaire, 91898 Orsay (France); Blin, S.; Cheikali, C.; Cuisy, D.; Gaspard, M.; Fournier, D.; Heller, M. [Laboratoire de l' Accelerateur Lineaire, 91898 Orsay (France); Iwanski, W. [Institute of Nuclear Physics PAN, Radzikowskiego 152, 31-342 Cracow (Poland); Lavigne, B.; De la Taille, C.; Puzo, P.; Socha, J-L. [Laboratoire de l' Accelerateur Lineaire, 91898 Orsay (France)

    2010-11-01

    The front end electronic (PMF) of the future ATLAS luminometer is described here. It is composed of a MAPMT and a compact stack of three PCBs, which deliver high voltage, route and read out of the output signals. The third board contains an FPGA and MAROC, a 64-channel ASIC, which can correct the non-uniformity of the MAPMT channels gain, thanks to a variable gain preamplifier. Its main role is to shape and discriminate the input signals at 1/3 photo-electron and produce 64 trigger outputs. Laboratory tests performed on prototype and pre-series PMFs have showed performances in good agreement with the requirements and have fulfilled the approval criteria for the final production of all elements.

  10. A first-year dornase alfa treatment impact on clinical parameters of patients with cystic fibrosis: the Brazilian cystic fibrosis multicenter study

    Directory of Open Access Journals (Sweden)

    Tatiana Rozov

    2013-12-01

    Full Text Available OBJECTIVE: To describe the clinical impact of the first year treatment with dornase alfa, according to age groups, in a cohort of Brazilian Cystic Fibrosis (CF patients. METHODS: The data on 152 eligible patients, from 16 CF reference centers, that answered the medical questionnaires and performed laboratory tests at baseline (T0, and at six (T2 and 12 (T4 months after dornase alfa initiation, were analyzed. Three age groups were assessed: six to 11, 12 to 13, and >14 years. Pulmonary tests, airway microbiology, emergency room visits, hospitalizations, emergency and routine treatments were evaluated. Student's t-test, chi-square test and analysis of variance were used when appropriated. RESULTS: Routine treatments were based on respiratory physical therapy, regular exercises, pancreatic enzymes, vitamins, bronchodilators, corticosteroids, and antibiotics. In the six months prior the study (T0 phase, hospitalizations for pulmonary exacerbations occurred in 38.0, 10.0 and 61.4% in the three age groups, respectively. After one year of intervention, there was a significant reduction in the number of emergency room visits in the six to 11 years group. There were no significant changes in forced expiratory volume in one second (VEF1, in forced vital capacity (FVC, in oxygen saturation (SpO2, and in Tiffenau index for all age groups. A significant improvement in Shwachman-Kulczychi score was observed in the older group. In the last six months of therapy, chronic or intermittent colonization by P. aeruginosa was detected in 75.0, 71.4 and 62.5% of the studied groups, respectively, while S. aureus colonization was identified in 68.6, 66.6 and 41.9% of the cases. CONCLUSIONS: The treatment with dornase alfa promoted the maintenance of pulmonary function parameters and was associated with a significant reduction of emergency room visits due to pulmonary exacerbations in the six to 11 years age group, with better clinical scores in the >14 age group, one

  11. A first-year dornase alfa treatment impact on clinical parameters of patients with cystic fibrosis: the Brazilian cystic fibrosis multicenter study

    Science.gov (United States)

    Rozov, Tatiana; Silva, Fernando Antônio A. e; Santana, Maria Angélica; Adde, Fabíola Villac; Mendes, Rita Heloisa

    2013-01-01

    OBJECTIVE: To describe the clinical impact of the first year treatment with dornase alfa, according to age groups, in a cohort of Brazilian Cystic Fibrosis (CF) patients. METHODS: The data on 152 eligible patients, from 16 CF reference centers, that answered the medical questionnaires and performed laboratory tests at baseline (T0), and at six (T2) and 12 (T4) months after dornase alfa initiation, were analyzed. Three age groups were assessed: six to 11, 12 to 13, and >14 years. Pulmonary tests, airway microbiology, emergency room visits, hospitalizations, emergency and routine treatments were evaluated. Student's t-test, chi-square test and analysis of variance were used when appropriated. RESULTS: Routine treatments were based on respiratory physical therapy, regular exercises, pancreatic enzymes, vitamins, bronchodilators, corticosteroids, and antibiotics. In the six months prior the study (T0 phase), hospitalizations for pulmonary exacerbations occurred in 38.0, 10.0 and 61.4% in the three age groups, respectively. After one year of intervention, there was a significant reduction in the number of emergency room visits in the six to 11 years group. There were no significant changes in forced expiratory volume in one second (VEF1), in forced vital capacity (FVC), in oxygen saturation (SpO2), and in Tiffenau index for all age groups. A significant improvement in Shwachman-Kulczychi score was observed in the older group. In the last six months of therapy, chronic or intermittent colonization by P. aeruginosa was detected in 75.0, 71.4 and 62.5% of the studied groups, respectively, while S. aureus colonization was identified in 68.6, 66.6 and 41.9% of the cases. CONCLUSIONS: The treatment with dornase alfa promoted the maintenance of pulmonary function parameters and was associated with a significant reduction of emergency room visits due to pulmonary exacerbations in the six to 11 years age group, with better clinical scores in the >14 age group, one year after the

  12. DIC Complicating APL Successfully Treated With Recombinant Thrombomodulin Alfa.

    Science.gov (United States)

    Saito, Aki; Okamoto, Yasuhiro; Seki, Yuko; Matsunaga, Manaka; Nakagawa, Shunsuke; Kodama, Yuichi; Nishikawa, Takuro; Tanabe, Takayuki; Kawano, Yoshifumi

    2016-08-01

    An 8-year-old boy developed anorexia, fatigue, and fever. Laboratory examination revealed a high white blood cell (WBC) count of 145×10/μL with 97.5% abnormal promyelocytic cells that contained Auer bodies. Faggot cells were seen. He was diagnosed with acute promyelocytic leukemia. Later, a chromosome analysis showed 46,XY,t(15;17)(q22;q12). Promyelocytic Leukemia-retinoic acid receptor α-fused gene and chimeric mRNA were confirmed by fluorescence in situ hybridization and reverse transcriptase polymerase chain reaction, respectively. He was complicated with disseminated intravascular coagulation (DIC) and his fibrin and fibrinogen degradation product at the onset was 37.6 μg/mL. Human recombinant thrombomodulin (rTM) was started for DIC. After dexamethasone was administered at a dose of 8 mg/m to prevent all-trans retinoic acid syndrome on day 1, all-trans retinoic acid was started at a dose of 45 mg/m on day 4. Cytarabine (100 mg/m/d) and daunorubicin (45 mg/m/d) were started on day 9. The WBC count gradually increased to 270×10/μL on day 8, and then decreased beginning on day 9. DIC improved after the initiation of chemotherapy and only minor petechia was noted. DIC did not become worse even after rTM was stopped on day 8. The risk of DIC and bleeding is high in the early stage of treatment for acute promyelocytic leukemia, especially in patients with a high WBC count. In our patient, rTM may have prevented fatal DIC and made it possible to safely administer induction chemotherapy. PMID:27123666

  13. The Arecibo Legacy Fast ALFA Survey : X. The HI Mass Function and Omega_HI From the 40% ALFALFA Survey

    NARCIS (Netherlands)

    Martin, Ann M.; Papastergis, Emmanouil; Giovanelli, Riccardo; Haynes, Martha P.; Springob, Christopher M.; Stierwalt, Sabrina

    2010-01-01

    The Arecibo Legacy Fast ALFA (ALFALFA) survey has completed source extraction for 40% of its total sky area, resulting in the largest sample of HI-selected galaxies to date. We measure the HI mass function from a sample of 10,119 galaxies with 6.2

  14. Molecular design and downstream processing of turoctocog alfa (NovoEight), a B-domain truncated factor VIII molecule

    Science.gov (United States)

    Ahmadian, Haleh; Hansen, Ernst B.; Faber, Johan H.; Sejergaard, Lars; Karlsson, Johan; Bolt, Gert; Hansen, Jens J.; Thim, Lars

    2016-01-01

    Turoctocog alfa (NovoEight) is a third-generation recombinant factor VIII (rFVIII) with a truncated B-domain that is manufactured in Chinese hamster ovary cells. No human or animal-derived materials are used in the process. The aim of this study is to describe the molecular design and purification process for turoctocog alfa. A five-step purification process is applied to turoctocog alfa: protein capture on mixed-mode resin; immunoaffinity chromatography using a unique, recombinantly produced anti-FVIII mAb; anion exchange chromatography; nanofiltration and size exclusion chromatography. This process enabled reduction of impurities such as host cell proteins (HCPs) and high molecular weight proteins (HMWPs) to a very low level. The immunoaffinity step is very important for the removal of FVIII-related degradation products. Manufacturing scale data shown in this article confirmed the robustness of the purification process and a reliable and consistent reduction of the impurities. The contribution of each step to the final product purity is described and shown for three manufacturing batches. Turoctocog alfa, a third-generation B-domain truncated rFVIII product is manufactured in Chinese hamster ovary cells without the use of animal or human-derived proteins. The five-step purification process results in a homogenous, highly purified rFVIII product. PMID:26761578

  15. Il trattamento di pazienti con sepsi grave mediante drotrecogin alfa: una valutazione economica con riferimento all’Italia

    Directory of Open Access Journals (Sweden)

    Carlo Lucioni

    2002-09-01

    Full Text Available Sepsis can be defined as a spectrum of clinical conditions caused by the immune response of a host to infection or trauma and characterized by systemic inflammation and coagulation. Particularly in elderly, immunocompromised and critically ill patients, sepsis is a major cause of morbidity and mortality in intensive care units (ICUs worldwide. In the US, sepsis is the leading cause of death in noncoronary ICU patients. Drotrecogin alfa, or recombinant human activated protein C, has antithrombotic, antiinflammatory, and profibrinolytic properties. Recently in a phase III trial (PROWESS, Drotrecogin alfa demonstrated significantly reduced mortality in severe sepsis patients at 28 days. In this trial important value factors for the assessment of costs and outcomes of severe sepsis were also considered. The purpose of the present study is to determine the economic burden of the treatment with Drotrecogin alfa, according to a cost-effectiveness analysis based on the data of the PROWESS trial. The study has been adapted to the italian health environment. As regards to the costs per surviving patient and costs per QALY (Quality Adjusted Life Year, the predicted cost-effectiveness ratio of drotrecogin alfa in severe sepsis patients is much lower than the standard values considered as acceptable in the international litterature.

  16. Characterization of IXINITY® (Trenonacog Alfa), a Recombinant Factor IX with Primary Sequence Corresponding to the Threonine-148 Polymorph.

    Science.gov (United States)

    Monroe, Dougald M; Jenny, Richard J; Van Cott, Kevin E; Buhay, Shelly; Saward, Laura L

    2016-01-01

    The goal of these studies was to extensively characterize the first recombinant FIX therapeutic corresponding to the threonine-148 (Thr-148) polymorph, IXINITY (trenonacog alfa [coagulation factor IX (recombinant)]). Gel electrophoresis, circular dichroism, and gel filtration were used to determine purity and confirm structure. Chromatographic and mass spectrometry techniques were used to identify and quantify posttranslational modifications. Activity was assessed as the ability to activate factor X (FX) both with and without factor VIIIa (FVIIIa) and in a standard clotting assay. All results were consistent across multiple lots. Trenonacog alfa migrated as a single band on Coomassie-stained gels; activity assays were normal and showed factor IX (FIXa) per IU of FIX. The molecule has >97%  γ-carboxylation and underwent the appropriate structural change upon binding calcium ions. Trenonacog alfa was activated normally with factor XIa (FXIa); once activated it bound to FVIIIa and FXa. When activated to FIXa, it was inhibited efficiently by antithrombin. Glycosylation patterns were similar to plasma-derived FIX with sialic acid content consistent with the literature reports of good pharmacokinetic performance. These studies have shown that trenonacog alfa is a highly pure product with a primary sequence and posttranslational modifications consistent with the common Thr-148 polymorphism of plasma-derived FIX. PMID:26997955

  17. Characterization of IXINITY® (Trenonacog Alfa, a Recombinant Factor IX with Primary Sequence Corresponding to the Threonine-148 Polymorph

    Directory of Open Access Journals (Sweden)

    Dougald M. Monroe

    2016-01-01

    Full Text Available The goal of these studies was to extensively characterize the first recombinant FIX therapeutic corresponding to the threonine-148 (Thr-148 polymorph, IXINITY (trenonacog alfa [coagulation factor IX (recombinant]. Gel electrophoresis, circular dichroism, and gel filtration were used to determine purity and confirm structure. Chromatographic and mass spectrometry techniques were used to identify and quantify posttranslational modifications. Activity was assessed as the ability to activate factor X (FX both with and without factor VIIIa (FVIIIa and in a standard clotting assay. All results were consistent across multiple lots. Trenonacog alfa migrated as a single band on Coomassie-stained gels; activity assays were normal and showed 97%  γ-carboxylation and underwent the appropriate structural change upon binding calcium ions. Trenonacog alfa was activated normally with factor XIa (FXIa; once activated it bound to FVIIIa and FXa. When activated to FIXa, it was inhibited efficiently by antithrombin. Glycosylation patterns were similar to plasma-derived FIX with sialic acid content consistent with the literature reports of good pharmacokinetic performance. These studies have shown that trenonacog alfa is a highly pure product with a primary sequence and posttranslational modifications consistent with the common Thr-148 polymorphism of plasma-derived FIX.

  18. Accurate Learning with Few Atlases (ALFA): an algorithm for MRI neonatal brain extraction and comparison with 11 publicly available methods

    Science.gov (United States)

    Serag, Ahmed; Blesa, Manuel; Moore, Emma J.; Pataky, Rozalia; Sparrow, Sarah A.; Wilkinson, A. G.; MacNaught, Gillian; Semple, Scott I.; Boardman, James P.

    2016-03-01

    Accurate whole-brain segmentation, or brain extraction, of magnetic resonance imaging (MRI) is a critical first step in most neuroimage analysis pipelines. The majority of brain extraction algorithms have been developed and evaluated for adult data and their validity for neonatal brain extraction, which presents age-specific challenges for this task, has not been established. We developed a novel method for brain extraction of multi-modal neonatal brain MR images, named ALFA (Accurate Learning with Few Atlases). The method uses a new sparsity-based atlas selection strategy that requires a very limited number of atlases ‘uniformly’ distributed in the low-dimensional data space, combined with a machine learning based label fusion technique. The performance of the method for brain extraction from multi-modal data of 50 newborns is evaluated and compared with results obtained using eleven publicly available brain extraction methods. ALFA outperformed the eleven compared methods providing robust and accurate brain extraction results across different modalities. As ALFA can learn from partially labelled datasets, it can be used to segment large-scale datasets efficiently. ALFA could also be applied to other imaging modalities and other stages across the life course.

  19. Benefit of adjuvant interferon alfa-2b (IFN-α) therapy in melanoma patients with high serum MMP-8 levels

    DEFF Research Database (Denmark)

    Vihinen, Pia; Tervahartiala, Taina; Sorsa, Timo;

    2015-01-01

    Matrix metalloproteinases (MMPs) are important enzymes in tissue turnover and various inflammatory processes. In this study, it was evaluated whether serum MMP-8 can predict the response to adjuvant interferon alfa-2b (IFN-α) therapy in patients with operated high-risk cutaneous melanoma. Pre...

  20. Dificuldades na identificação laboratorial da talassemia alfa Difficulty on laboratory identification of alpha thalassemia

    Directory of Open Access Journals (Sweden)

    Karlla Greick Batista Dias-Penna

    2010-04-01

    Full Text Available Introdução: Talassemia alfa é uma síndrome associada à redução da síntese de cadeias de globina do tipo alfa. A gravidade das manifestações clínicas está relacionada com a quantidade de globinas produzida e a estabilidade das cadeias beta presentes em excesso. A talassemia alfa mínima resulta da deleção de apenas um dos quatro genes a (-α/αα. Clinicamente apresenta anemia leve com microcitose ou ausência de anemia, sendo o diagnóstico realizado por meio de visualização da hemoglobina (Hb H por eletroforese alcalina em acetato de celulose ou por identificação de inclusões celulares de Hb H coradas pelo azul de crezil brilhante. Objetivo: Avaliar portadores de talassemia alfa e seus respectivos progenitores, correlacionando perfil hematológico e presença de Hb H, utilizando procedimentos laboratoriais clássicos em três diferentes amostragens. Discussão e conclusão: Os dados obtidos mostram que a presença de Hb H, indicativo de talassemia alfa, pode não ser confirmada em uma análise posterior. Entre os fatores que podem influenciar no não aparecimento de Hb H em pessoa comprovadamente com talassemia alfa está a deficiência de ferro. A talassemia alfa está associada a defeitos envolvendo os genes codificadores da cadeia alfa, mas também pode estar relacionada com desbalanciamento temporário na expressão dos genes globina, diminuição de alfa ou aumento de beta, o que poderia explicar o aparecimento de tetrâmeros de cadeia beta (Hb H, sugerindo diagnóstico de talassemia alfa mínima.Introduction: Alpha thalassemia is a syndrome with associated with the reduction of alpha globin chain synthesis. The severity of clinical manifestations is related to the amount of globins produced and the stability of beta chains that are present in excess. Alpha thalassemia minor is caused by the deletion of one of the four genes a (-α/αα. Clinically, it presents mild anemia with microcytosis or absence of anemia. The

  1. Unidades fototérmicas e temperatura-base inferior de frutos de Mangueira Alfa, na Baixada Cuiabana Photothermal units and lower base temperature for alfa mango fruit on the Baixada Cuiabana

    Directory of Open Access Journals (Sweden)

    Marcelo Paes de Barros

    2010-06-01

    Full Text Available O objetivo deste trabalho foi determinar a temperatura-base inferior (Tb a partir da unidade fototérmica (UF para o cultivo experimental de manga Alfa sob condições de cerrado. Foram utilizados dados diários da temperatura do ar disponibilizados pela Estação Agrometeorológica Padre Ricardo Remetter, localizada em Santo Antônio do Leverger-MT (15,8° S e 56,1° W, 140 m, e avaliação sensorial do estádio de maturação dos frutos de manga. A Tb foi determinada pelo método da menor variabilidade das unidades fototérmicas (UF acumuladas do período da floração à colheita dos frutos, variabilidade avaliada pelo coeficiente de variação (cv simulado para diferentes valores da Tb. De julho a novembro de 2007, em três plantas de um pomar demonstrativo irrigado, foram identificados 82 frutos para o acompanhamento do crescimento e maturação. Entre esses frutos, em meados de dezembro de 2007, foi possível identificar 13 frutos que atingiram a maturidade fisiológica, após um período médio de observação de 112 dias. Para exigência fototérmica de 1.878.166,1 UF, encontrou-se Tb de 10 °C, valor consistente com os apresentados na literatura para a cultivar de manga, o que comprova a eficiência do método que combina a ação da temperatura e do fotoperíodo sobre a maturação dos frutos e que confere um caráter mais racional que o método tradicional da soma térmica.In this study was to determine the lower base temperature (Tb for Alfa mango fruit, using the photothermal units (PTU method in cerrado conditions. The meteorological data for the study were acquired from "Padre Ricardo Remetter" meteorological station located at Santo Antonio do Leverger, MT, Brazil (15,8° S and 56,1° W, 140 m, and the fruit data was acquired by sensorial evaluation of mango fruit maturation state. The Tb was determined by less variability of the coefficient of variation (cv method applied to the PTU sum, from flowering to harvest, simulated for

  2. Dornase Alfa

    Science.gov (United States)

    ... is sometimes prescribed for other uses; ask your doctor or pharmacist for more information. ... written instructions that come with it. Ask your doctor, pharmacist, or respiratory therapist to demonstrate the proper technique. ...

  3. L'alfa (Stipa tenacissima L. dans la plaine de Midelt (haut bassin versant de la Moulouya, Maroc – Éléments de climatologie

    Directory of Open Access Journals (Sweden)

    Mustapha Rhanem

    2009-01-01

    Full Text Available L'alfa (Stipa tenacissima L. est représenté dans la haute plaine de Midelt par des touffes en bon état et de grande taille pouvant atteindre parfois des hauteurs allant jusqu'à 1,20 m, avec une densité moyenne à forte. Il y constitue un écosystème steppique intramontagnard spontané, caractérisé par une bonne régénération qui témoigne de conditions topo-édapho-climatiques propices à sa croissance et à sa reproduction.L'approche climatique utilisée pour caractériser cette steppe de plaine (par opposition à la steppe de versant est fondée sur deux critères climatiques : la pluviosité (et plus généralement les précipitations et la température. Chacun des deux paramètres a été analysé sous différents angles en ayant recours à différents indicateurs. Ces critères ne sont pas les seuls facteurs climatiques agissant sur les conditions de vie de l'alfa et de son cortège d'espèces végétales steppiques ; mais ils sont prédominants. En effet, ces facteurs présentent d'étroites corrélations avec d'autres paramètres climatiques, tels que l'humidité relative, le vent et l'insolation. Enfin, le rapport P/2T de H. GAUSSEN a été utilisé pour cerner les modalités de la sécheresse.In Midelt high plain, alfa (Stipa tenacissima L. is formed by mean to high density good state of health and tall tufts that can sometimes reach up to 1.2 m height. It there builds up a spontaneous steppic intramountain ecosystem, characterized by a good regeneration that attests topo-edapho-climatic conditions are favorable to its growth and reproduction. Climatic approach used for characterizing this plain steppe (in contrast with slope steppe is based on two climatic criteria: raininess (and more generally rainfalls and temperature. Each of these two parameters has been analysed from different points of view using different indicators. These criteria are not the only climatic factors that act on the life conditions of alfa and its

  4. Medida de actividades Alfa y Beta en muestras ambientales con un detector proporcional y detección de 99Tc

    OpenAIRE

    García de León, Manuel

    1983-01-01

    Se utiliza un detector proporcional de flujo de gas para la medida de actividades Alfa y Beta ambientales y la detección de 99Tc. Se estudia la respuesta del detector a la radiación beta relacionando de una manera sistemática la energía máxima del espectro del emisor beta con la forma del platean producido. De la misma manera se estudia la medida de partículas alfa. Haciendo las correcciones por efecto de Crosstalk se propone un método de recuento simultaneo de radiación Alfa y Beta. Y se ap...

  5. Interferon Stimulated Gene Expression in HIV/HCV Coinfected Patients Treated with Nitazoxanide/Peginterferon-Alfa-2a and Ribavirin.

    Science.gov (United States)

    Petersen, Tess; Lee, Yu-Jin; Osinusi, Anu; Amorosa, Valerianna K; Wang, Crystal; Kang, Minhee; Matining, Roy; Zhang, Xiao; Dou, Diana; Umbleja, Triin; Kottilil, Shyam; Peters, Marion G

    2016-07-01

    A combination of nitazoxanide (NTZ), peginterferon (PegIFN), and ribavirin (RBV) may result in higher sustained virologic response (SVR) rates in hepatitis C virus (HCV) monoinfected patients. This study evaluated the effect of NTZ on interferon-stimulated gene (ISG) expression in vitro and in vivo among HIV/HCV genotype-1 (GT-1) treatment-naive patients. The ability of NTZ to enhance host response to interferon (IFN) signaling using the HCV cell culture system was initially evaluated. Second, ISG expression in 53 patients with treatment outcomes [21 SVR and 32 nonresponders (NR)] in the ACTG A5269 trial, a phase-II study (4-week lead in of NTZ 500 mg daily followed by 48 weeks of NTZ, PegIFN, and weight-based RBV), was assessed. The relative expression of 48 ISGs in peripheral blood mononuclear cells (PBMCs) was measured at baseline, week 4, and week 8 of treatment in a blinded manner. In vitro NTZ produced a direct and additive antiviral effect with IFN-alfa, with pretreatment of NTZ resulting in maximal HCV suppression. NTZ augmented IFN-mediated ISG induction in PBMCs from relapsers and SVRs (p < 0.05), but not NR. In ACTG A5269, baseline expression of most ISGs was similar between NR and SVR. NTZ minimally induced 17 genes in NR and 13 genes in SVR after 4 weeks of therapy. However, after initiation of PegIFN and RBV, ISG induction was predominantly observed in the SVR group and not NR group. NTZ treatment facilitates IFN-induced suppression of HCV replication. Inability to achieve SVR with IFN-based therapy in this clinical trial is associated with diminished ISG response to therapy that is refractory to NTZ. PMID:26974581

  6. Study on the 90m β* optics for the ALFA experiment at the LHC

    International Nuclear Information System (INIS)

    The ALFA experiment, which is located at the ATLAS interaction region, measures elastic scattering at very small angles in order to determine the absolute luminosity at the interaction point (IP). For that purpose, Roman Pot detectors are positioned very close to the LHC beam. The Luminosity determination requires very accurate knowledge of the accelerator optics in between the IP and the Roman Pots. Phase advances and beta functions can be measured by exciting beam oscillations and recording the turn-by-turn data with beam position monitors. Monte-Carlo simulations will be presented, which have been used to combine these optics measurements with constraints from different techniques, in order to find uncertainties in the model parameters.

  7. The ATLAS Forward Detectors - LUCID, ALFA and AFP: Past, Present and Future

    CERN Document Server

    Caforio, D; The ATLAS collaboration

    2013-01-01

    LUCID is a gas Cerenkov detector deployed near to the beam‐pipe at 17 m either side of the ATLAS Intersection point. The LUCID detector ‐ when calibrated via a Van der Meer scan – is now measuring absolute luminosity on a bunch‐by‐bunch basis. It is now measuring integrated luminosity up to high luminosity with a precision of around 3%. The technical challenges that had to faced to make this measurement as well as upgrade plans for LUCID will be presented. In addition to LUCID, we will discuss that ALFA detector (Absolute Luminosity for ATLAS) and its status as well as the progress on the ATLAS Forward Protons project (AFP) that plans to deploy detectors to tag and measure, with 3‐D silicon detectors, both protons in exclusive central diffractive processes.

  8. Concentração de alfa-tocoferol no soro e colostro materno de adolescentes e adultas Levels of alpha-tocopherol in maternal serum and colostrum of adolescents and adults

    Directory of Open Access Journals (Sweden)

    Roberto Dimenstein

    2010-06-01

    Full Text Available OBJETIVOS: avaliar a concentração de alfa-tocoferol no soro e colostro materno de puérperas adolescentes e adultas e verificar a adequação nutricional de vitamina E do colostro oferecido ao lactente. MÉTODOS: participaram do estudo 72 puérperas, sendo 25 adolescentes e 47 adultas. Foram coletados 5 mL de sangue e 2 mL de colostro em condição de jejum para análise dos níveis de alfa-tocoferol. As amostras foram analisadas por cromatografia líquida de alta eficiência. A adequação nutricional do colostro para a vitamina E foi calculada pelo produto do volume estimado de ingestão de leite com a concentração de alfa-tocoferol no colostro e por comparação direta desse produto com o valor de referência para ingestão do nutriente (4 mg/dia. RESULTADOS: os níveis de alfa-tocoferol no soro de puérperas adolescentes e adultas foram, respectivamente, 30,8±9,8 e 34,1±9,5 µmol/L (média±DP. No colostro, as adolescentes apresentaram concentração de alfa-tocoferol de 32,9±15,8 µmol/L e as adultas de 30,4 ± 18,0 µmol/L, não sendo encontrada diferença significativa entre as concentrações séricas, assim como no colostro dos grupos estudados. CONCLUSÕES: Tanto as puérperas adolescentes quanto as adultas apresentaram um estado nutricional satisfatório de vitamina E refletido no colostro cujos valores foram capazes de suprir o requerimento nutricional do lactente, sugerindo que a idade materna não influencia os níveis de alfa-tocoferol do colostro humano.PURPOSE: to assess the alpha-tocopherol concentration in the serum and colostrum of adolescent and adult mothers and to determine the nutritional adequacy of vitamin E in the colostrum offered to infants. METHODS: in total, 72 pregnant women participated in the study, 25 adolescents and 47 adults. An amount of 5 mL of blood and 2 mL of colostrum were collected under fasting conditions for the analysis of alpha-tocopherol levels. The samples were analyzed by means of high

  9. Effect of Epoetin Alfa Dose Changes on Hemoglobin and Mortality in Hemodialysis Patients with Hemoglobin Levels Persistently below 11 g/dL

    OpenAIRE

    Bradbury, Brian D.; Danese, Mark D.; Gleeson, Michelle; Critchlow, Cathy W.

    2009-01-01

    Background and objectives: The mortality risk associated with attempting to raise hemoglobin (Hb) levels by increasing Epoetin alfa (EPO) doses in hemodialysis patients with persistently low Hb remains poorly understood.

  10. Predictive factors for ovarian response in a corifollitropin alfa/GnRH antagonist protocol for controlled ovarian stimulation in IVF/ICSI cycles

    OpenAIRE

    Oehninger, Sergio; Nelson, Scott M.; Verweij, Pierre; Stegmann, Barbara J.

    2015-01-01

    Background This secondary analysis aimed to identify predictors of low (18 oocytes retrieved) in IVF patients undergoing controlled ovarian stimulation with corifollitropin alfa in a gonadotropin-releasing hormone (GnRH) antagonist protocol. Methods Statistical model building for high and low ovarian response was based on the 150 μg corifollitropin alfa treatment group of the Pursue trial in infertile women aged 35–42 years (n = 694). Results Multivariable logistic regression models were cons...

  11. A first-year dornase alfa treatment impact on clinical parameters of patients with cystic fibrosis: the Brazilian cystic fibrosis multicenter study

    OpenAIRE

    Tatiana Rozov; Fernando Antônio A. e Silva; Maria Angélica Santana; Fabíola Villac Adde; Rita Heloisa Mendes

    2013-01-01

    OBJECTIVE: To describe the clinical impact of the first year treatment with dornase alfa, according to age groups, in a cohort of Brazilian Cystic Fibrosis (CF) patients. METHODS: The data on 152 eligible patients, from 16 CF reference centers, that answered the medical questionnaires and performed laboratory tests at baseline (T0), and at six (T2) and 12 (T4) months after dornase alfa initiation, were analyzed. Three age groups were assessed: six to 11, 12 to 13, and >14 years. Pulmonar...

  12. Teores de retinol, beta-caroteno e alfa-tocoferol em leites bovinos comercializados na cidade de São Paulo Amounts of retinol, beta-carotene and alpha-tocopherol in cow milk comercialized in the city of São Paulo

    OpenAIRE

    BIANCHINI Rute; Marilene de Vuono Camargo PENTEADO

    1999-01-01

    Os teores de retinol, beta-caroteno e alfa-tocoferol foram determinados por cromatografia líquida de alta eficiência em leites em pó, pasteurizados e esterilizados, comercializados na Cidade de São Paulo. Após a saponificação e extração, os compostos foram determinados simultaneamente utilizando-se coluna de sílica, fase móvel constituída por hexano:isopropanol (99:1) e fluxo de 2,0mL/min. O retinol e o beta-caroteno foram determinados no detector UV/visível e o alfa-tocoferol no detector de ...

  13. Purificação e caracterização de alfa-galactosidases de sementes de Platymiscium pubescens Micheli Purification and characterization of alpha-galactosidases from Platymiscium pubescens Micheli seeds

    Directory of Open Access Journals (Sweden)

    Giordani de Oliveira

    2005-08-01

    Full Text Available Este trabalho objetivou foi determinar a composição bioquímica de sementes de espécies florestais e caracterizar a enzima alfa-galactosidase de sementes germinadas de Platymiscium pubescens. Os maiores teores de lipídios foram determinados em sementes de Chorisia speciosa, Caesalpinia peltophoroides, Tabebuia serratifolia e Tabebuia velanedae, enquanto sementes de Enterolobium contortisiliquum, Schizolobium parahyba e Cassia grandis apresentaram os maiores teores protéicos. A alfa-galactosidase catalisa a hidrólise dos oligossacarídeos de rafinose, em sementes de leguminosas, durante a germinação. A maior atividade da alfa-galactosidase foi detectada em sementes de Platymiscium pubescens após 72 h de embebição. Duas formas de alfa-galactosidases, C1 e C2, foram purificadas de sementes germinadas de P. pubescens, usando-se fracionamento com sulfato de amônio e cromatografias de filtração em gel e de afinidade. Essas enzimas apresentaram atividade máxima em pH 5,5 e a 50-55 ºC. Os valores de Km ap das formas C1 e C2, para o substrato ro-nitrofenil-alfa-D-galactopiranosídeo, foram de 0,54 mM e 0,78 mM, e para a rafinose, de 4,64 mM e 5,09 mM, respectivamente. Essas enzimas exibiram estabilidade térmica moderada, mantendo 70% da atividade original após 3 h de incubação a 45 ºC. A atividade enzimática da C1 e C2 foi totalmente perdida na presença de CuSO4 e dodecil sulfato de sódio (SDS. Tais enzimas também hidrolisaram melibiose, rafinose e estaquiose, indicando potencial para aplicações biotecnológicas.The objective of this work was to determine seed biochemical composition of forest species and to characterize alpha-galactosidase enzyme of germinated seeds of Platymiscium pubescens. The highest lipid levels were found in seeds of Chorisia speciosa, Caesalpinia peltophoroides, Tabebuia serratifolia and Tabebuia velanedae, whereas seeds of Enterolobium contortisiliquum, Schizolobium parahyba and Cassia grandis showed

  14. Alglucosidase alfa: Long term use in the treatment of patients with Pompe disease

    Directory of Open Access Journals (Sweden)

    Michael Beck

    2009-09-01

    Full Text Available Michael BeckChildren’s Hospital, University of Mainz, Mainz, GermanyAbstract: Pompe disease is a lysosomal storage disorder characterized by muscle weakness and cardiomyopathy. It shows a broad variability regarding the clinical severity as well as the age of onset. In the past, two different recombinant enzyme preparations have been developed for the treatment of Pompe patients: α-glucosidase, produced in rabbit milk, and α-glucosidase, produced in Chinese hamster ovary (CHO cell lines. The CHO enzyme received marketing approval in 2006 after it was proven to be effective in ameliorating muscle strength and improving heart function. The other has not been approved. The clinical efficacy of this enzyme preparation could be confirmed by several clinical trials in patients with different age of onset and disease severity. Enzyme replacement therapy, however, has its limitations due to unsatisfactory access of recombinant α-glucosidase to the muscle cells and due to the formation of antibodies. To overcome these therapeutic restraints, the development of a more effective enzyme preparation may become necessary.Keywords: alglucosidase alfa, alpha glucosidase, Pompe disease, enzyme replacement therapy, glycogen storage disorder type II, acid maltase deficiency

  15. Profile of alglucosidase alfa in the treatment of Pompe disease: safety, efficacy, and patient acceptability

    Directory of Open Access Journals (Sweden)

    Schneider I

    2016-01-01

    Full Text Available Ilka Schneider, Stephan Zierz Department of Neurology, Martin Luther University Halle-Wittenberg, Halle (Saale, Germany Abstract: Pompe disease, also referred to as glycogenosis type II, is a rare, autosomal recessive disorder that results from the deficiency of the glycogen-degrading enzyme acid α-glucosidase. The classical form presents shortly after birth with muscle hypotonia, cardiac, and respiratory failure resulting in a fatal outcome. The late onset of Pompe disease has a very variable onset and disease presentation that often causes a delayed diagnosis. Until now enzyme replacement therapy with alglucosidase alfa is the only causative therapy option for Pompe patients that can slow down disease progression. However, uncertainty remains about the efficacy regarding survival and quality of life in Pompe patients under this very cost-intensive treatment. This paper provides a systematic review of the literature stressing different aspects of enzyme replacement therapy in infantile and late onset Pompe patients. Keywords: lysosomal storage disease, glycogenosis type II, acid maltase deficiency, IOPD, LOPD, enzyme replacement therapy

  16. Development of solar energy education IASEE-Argentina, Arquisur and Alfa-built

    International Nuclear Information System (INIS)

    Three educational experiences are presented in this paper on the development of national and regional networks to incorporate renewable energy in curricular programmes. IASEE-Argentina, established in 1990 as the National Section of IASEE, the International Association for Solar Energy Education, was designated in 1993 as the Education Working Group of ASADES, the Argentine Association of Solar Energy. It holds annual meetings to coincide with the ASADES conference. Arquisur is a regional network established by Architectural Faculties of State Universities within the Mercosur Region, covering Argentina, southern Brazil, Paraguay and Uruguay, to promote exchange and development between the 19 faculties involved. A Working Group set up by Arquisur with members from four countries developed the programme for short post-graduate courses on bioclimatic design and rational energy use in buildings, which has been given in Argentina and Uruguay. Alfa-Built, a project supported by the European Union for promoting academic exchange in the field of energy efficient building is also introduced. This paper presents the development and initial results of these educational experiences. (author)

  17. O papel do Fator de Necrose Tumoral Alfa (TNF-alfa) no processo de erosão óssea presente no colesteatoma adquirido da orelha média The role of Tumor Necrosis Factor -Alpha (TNF- alpha) in bone resorption present in middle ear cholesteatoma

    OpenAIRE

    Rodrigo Faller Vitale; Fernando de Andrade Quintanilha Ribeiro

    2007-01-01

    O colesteatoma adquirido da orelha média causa erosão óssea, com altas taxas de morbidade e mortalidade. O TNF-alfa (TNF-alfa) lambda uma das principais citocinas envolvidas neste processo. OBJETIVO: Avaliar o papel do TNF-alfa na reabsorsão óssea e a ação dele no colesteatoma. MATERIAL E MÉTODOS: Foi realizado um levantamento e uma revisão crítica da literatura. RESULTADOS: Todos os autores estudados concordam com a importância do TNF-alfa no processo de reabsorção óssea presente no colestea...

  18. Associação de bronquite plástica com enteropatia perdedora de proteínas após operação de Fontan Asociación de bronquitis plástica a la enteropatía perdedora de proteínas tras operación de fontan Association of plastic bronchitis to protein-losing enteropathy after fontan operation

    OpenAIRE

    Vanessa Alves Guimarães; Edmar Atik; Jussara Bianchi Castelli; Nana Miura Ikari; Ana Maria Thomaz; Antonio Augusto Barbosa Lopes

    2010-01-01

    Relatamos um caso incomum de associação de bronquite plástica (BP) com enteropatia perdedora de proteínas (EPP) em menina de 4 anos e 9 meses de idade. com dupla via de entrada de ventrículo único tipo esquerdo e concordância ventrículo-arterial submetida à cirurgia cavopulmonar total. com túnel lateral intracardíaco aos três anos. Tornaram-se chamativas a eliminação de molde brônquico de fibrina de 10 cm (BP) e a elevação de alfa-1-antitripsina de 52 mg/g de fezes. Em uso de sildenafila. pro...

  19. Short versus standard treatment with pegylated interferon alfa-2A plus ribavirin in patients with hepatitis C virus genotype 2 or 3: the cleo trial

    Directory of Open Access Journals (Sweden)

    Picardi Antonio

    2010-02-01

    Full Text Available Abstract Background In patients with chronic hepatitis C virus (HCV genotype 2 or 3, 24 weeks' treatment with pegylated interferon alfa (PEG-IFN-alpha and ribavirin induces a sustained virological response (SVR in almost 80% of cases. Evidence suggests that a similar response rate may be obtained with shorter treatment periods, especially in patients with a rapid virological response (RVR. The aim of this study was to compare the efficacy of 12 or 24 weeks of treatment in patients with chronic HCV genotype 2 or 3 and to identify patients suitable for 12 weeks treatment. Methods Two hundred and ten patients received PEG-IFN-alpha-2a (180 ug/week and ribavirin (800-1200 mg/day for 4 weeks. Patients with a RVR (HCV RNA not detectable were randomized (1:1 to either 12 (group A1 or 24 (group A2 weeks of combination therapy. Patients without a RVR continued with 24-weeks' combination therapy (group B. HCV RNA was monitored at weeks 4, 8, 12, and 24, and at week 24 post-treatment. Results At study end, end of treatment response (ETR was observed in 62 (86% patients of group A1 and in 55 (77% patients of group A2 (p Conclusion In HCV patients with genotype 2 or 3, 12-week combination therapy is as efficacious as 24-week therapy and several independent covariates were predictive of SVR. Trial registration Trial number ISRCTN29259563

  20. Measurement of the total cross section in proton proton collisions at $\\mathbf{\\sqrt{s}=7}$ TeV with the ALFA sub-detector of ATLAS

    CERN Document Server

    Adamczyk, Leszek; The ATLAS collaboration

    2015-01-01

    Measurement of the total pp cross section at the LHC at $\\sqrt{s} = 7$ TeV is presented. In a special run with high-$\\beta^*$ beam optics, an integrated luminosity of 80 $\\mu$b$^{−1}$ was accumulated in order to measure the differential elastic cross section as a function of the Mandelstam momentum transfer variable $t$. The measurement is performed with the ALFA sub-detector of ATLAS. Using a fit to the differential elastic cross section in the $|t|$ range from 0.01 GeV$^2$ to 0.1 GeV$^2$ to extrapolate to $|t|\\rightarrow 0$, the total cross section, $\\sigma_{tot}(pp \\rightarrow X)$, is measured via the optical theorem to be: $\\sigma_{tot}(pp \\rightarrow X) = 95.35 \\pm 0.38$ (stat.) $\\pm 1.25 $ (exp.) $\\pm 0.37$ (extr.) mb , where the first error is statistical, the second accounts for all experimental systematic uncertainties and the last is related to uncertainties in the extrapolation to $|t|\\rightarrow 0$ . In addition, the slope of the elastic cross section at small $t$ is determined to be $B = 19.73 ...

  1. Análise comparativa do controle interno no contas a receber e a pagar nas empresas Beta Ltda e Alfa Ltda = A comparative internal control analysis of receivable and payable accounts in Beta and Alfa Ltd Companies

    Directory of Open Access Journals (Sweden)

    Cleyton de Oliveira Ritta

    2010-07-01

    Full Text Available A necessidade constante de um excelente desempenho empresarial leva à busca da qualidade e confiabilidade da informação para o auxílio na tomada de decisão. Neste contexto, a auditoria contábil tem um papel importante na análise das práticas administrativas adotadas pelas entidades. Desse modo, o objetivo deste artigo é comparar a estruturação dos controles internos no contas a receber e a pagar nas empresas Beta Ltda, a qual não contrata serviços de auditoria externa, e Alfa Ltda, que os contrata. Este estudo caracteriza-se como de caso comparativo de caráter descritivo por meio de questionário e entrevista. Os resultados apontam que a empresa Alfa Ltda tem melhores níveis de controle interno e, consequentemente, está menos exposta a erros e fraudes. Desse modo, entende-se que os seviços de auditoria atuam como um instrumento de auxílio para minimização dos riscos operacionais e servem de alerta aos gestores quanto à eficiência das transações. Contudo, percebe-se que a qualidade dos controles internos nas organizações depende diretamente da utilização de procedimentos de monitoramento.The constant need for excellent business performance triggers the quest for information quality and reliability to help in decision making. Considering this context, accounting auditing plays an important role in the analysis of administrative practices adopted by entities. Thus, the objective of this article is to compare the structure of the internal controls for receivable and payable accounts in Beta Ltd companies, which do not contract external auditing services, and Alfa Ltd, which does. This study is a descriptive comparative case study, carried out through questionnaire and interview. The results have showed that Alfa Ltd has better internal control levels and consequently is less exposed to errors and fraud. This way, auditing services are seen as auxiliary instruments to minimize operational risks and serve to alert

  2. Evaluation of the efficacy and safety of three dosing regimens of agalsidase alfa enzyme replacement therapy in adults with Fabry disease

    Directory of Open Access Journals (Sweden)

    Goláň L

    2015-07-01

    Full Text Available Lubor Goláň,1 Ozlem Goker-Alpan,2 Myrl Holida,3 Ikka Kantola,4 Mariusz Klopotowski,5 Johanna Kuusisto,6 Aleš Linhart,1 Jacek Musial,7 Kathleen Nicholls,8 Derlis Gonzalez-Rodriguez,9 Reena Sharma,10 Bojan Vujkovac,11 Peter Chang,12 Anna Wijatyk12 1First Faculty of Medicine, Department of Cardiovascular Medicine, Charles University, Prague, Czech Republic; 2Lysosomal Research and Treatment Unit, Fairfax, VA, USA; 3Stead Family Department of Pediatrics, Division of Medical Genetics, University of Iowa Hospitals and Clinics, Iowa City, IA, USA; 4Division of Medicine, Turku University Hospital, Turku, Finland; 5Institute of Cardiology, Warsaw, Poland; 6Department of Medicine, Center for Medicine and Clinical Research, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland; 7Department of Internal Medicine, Jagiellonian University Medical College, Krakow, Poland; 8Department of Nephrology, Royal Melbourne Hospital and the University of Melbourne, VIC, Australia; 9Instituto Privado de Hematologia E Investigacion Clinica (IPHIC, Asuncion, Paraguay; 10Salford Royal NHS Foundation Trust, Salford, UK; 11General Hospital Slovenj Gradec, Slovenj Gradec, Slovenia; 12Shire, Lexington, MA, USA Purpose: Efficacy and safety of agalsidase alfa at 0.2 mg/kg weekly were compared with 0.2 mg/kg every other week (EOW. Exploratory analyses were performed for 0.4 mg/kg weekly.Patients and methods: This was a 53-week, Phase III/IV, multicenter, open-label study (NCT01124643 in treatment-naïve adults (≥18 years with Fabry disease. Inclusion criteria were left ventricular hypertrophy at baseline, defined as left ventricular mass indexed to height >50 g/m2.7 for males and >47 g/m2.7 for females. Primary endpoint was reduction of left ventricular mass indexed to height as assessed by echocardiography. Secondary endpoints included cardiac (peak oxygen consumption, 6-minute walk test, Minnesota Living with Heart Failure Questionnaire, New

  3. Correlação do nível de alfa-feto proteína, índice de sobrevida e recidiva tumoral em pacientes submetidosa transplante hepático Survival and tumor relapse rate according to alpha-fetoprotein level in patients submitted to liver transplantation

    Directory of Open Access Journals (Sweden)

    Elaine Cristina Ataide

    2011-03-01

    Full Text Available RACIONAL: O transplante hepático para carcinoma hepatocelular pode resultar em potencial cura e melhora da sobrevida comparado com operações conservadoras. OBJETIVO: Analisar os índices de recorrência e sobrevida em pacientes transplantados hepáticos por carcinoma hepatocelular e com níveis séricos de alfa-fetoproteína maiores que 200 ng/ml. MÉTODO: Foram analisados retrospectivamente 90 pacientes cirróticos com carcinoma hepatocelular submetidos à transplante hepático ortotópico entre 1997 e 2009. As lesões hepáticas foram diagnosticadas no pré-operatório por ultrassonografia com Doppler, tomografia computadorizada e níveis séricos de alfa fetoproteína. Os pacientes foram divididos em dois grupos de acordo com o nível de alfa-fetoproteína (menor ou maior que 200 ng/ml. O método de Kaplan-Meier foi usado para calcular a taxa de sobrevida. A análise de regressão Cox estudou os fatores preditivos de sobrevida. RESULTADOS: Pacientes com alfa-fetoproteína maior que 200 ng/ml (n=6 apresentaram menor taxa de sobrevida em um e cinco anos e na média de meses comparados com o grupo com alfa-fetoproteína menor que 200 ng/ml (n=84; respectivamente 35%, 18% e 11,8 meses contra 68%, 43% e 28,1 meses. Além disso, a taxa de recidiva foi 16,6% no primeiro grupo, e de 5,6% no outro. Observouse risco de óbito de 1% para cada 10 u de alfa-fetoproteína>200 ng/ml e para cada mm da maior medida de tumor acima de 28 mm. CONCLUSÃO: Os pacientes com valores séricos de alfa-fetoproteína maiores que 200 ng/ml demonstraram menores taxas de sobrevida, porém não foi preditivo de recidiva tumoral.BACKGROUND: Liver transplantation for hepatocellular carcinoma (HCC can result in a potential cure and greater survival than other less radical techniques. AIM: To analyze the survival and recurrence rate in liver transplant recipients with hepatocellular carcinoma and alpha-fetoprotein over 200 ng/ml. METHOD: Analysis, in this retrospective study

  4. Secondary bronchiolitis obliterans organizing pneumonia during treatment of chronic hepatitis C: role of pegylated interferon alfa-2a

    Directory of Open Access Journals (Sweden)

    Ronaldo Soares Martins

    2012-10-01

    Full Text Available The treatment of chronic hepatitis C has frequent side effects such as cytopenias and neuropsychiatric symptoms. However, pulmonary toxicity associated with interferon is rarely described. This paper describes the clinical case of a 67-year-old female patient with chronic hepatitis C who presented an acute onset of dry cough, dyspnoea, and fever 36 weeks after the use of pegylated interferon alfa-2a and ribavirin. The lung biopsy confirmed the diagnosis of a bronchiolitis obliterans organizing pneumonia (BOOP. Corticotherapy was initiated, with clinical and radiological improvement. This paper aims to advise physicians to this occasional, though severe, adverse event related to hepatitis C virus (HCV treatment.

  5. Peginterferon Lambda-1a, a New Therapeutic for Hepatitis C Infection, from Bench to Clinic

    OpenAIRE

    Andersen, Henrik; Meyer, Jeff; Freeman, Jeremy; Doyle, Sean E.; Klucher, Kevin; Miller, Dennis M.; Hausman, Diana; Hillson, Jan L.

    2013-01-01

    Chronic infection with hepatitis C virus (HCV) is estimated to affect approximately 3% of the world's population and cause 350,000 deaths each year. For a number of years, the standard of care has been combination therapy with recombinant alfa interferons—originally as native proteins but more recently as polyethyleneglycol-modified derivatives—and ribavirin, with the recent addition of an NS3 protease inhibitor for HCV genotype 1. However, therapeutic alfa interferons are associated with a s...

  6. Alfa-Pinen, Terpineol, Sineol, Linelol ve Kamforun Bakteriyel Hücreler Arası İletişim Sistemi Üzerine İnhibitör Etkileri

    Directory of Open Access Journals (Sweden)

    Erdi DOĞRU

    2016-07-01

    Full Text Available Pek çok Gram negatif patojen bakteri çeşitli virülens faktörlerinin üretiminin genetik seviyede kontrolünü N-Açil homoserin lakton aracılı hücreler arası iletişim sistemini kullanarak sağlar. Bu nedenle bu hücreler arası iletişim sisteminin engellenmesi veya modifiye edilmesi, bu sistemi yeni terapötiklerin keşfi için cazip bir hedef haline getirmektedir. Hücreler arası iletişim sistemi inhibitörü, bitkisel kökenli pek çok doğal ürün bulunmaktadır. Bu çalışmada defne, adaçayı, mersin ve ardıç uçucu yağlarının bakterilerdeki hücreler arası iletişim sistemi üzerine engelleyici özellikleri incelenmiş ve bu yağ örneklerinin majör bileşenleri GC-MS ile belirlenmiştir. Alfa pinen, terpineol, sineol, linelol ve kamforun (yağ örneklerinin majör bileşenleri hücreler arası iletişim sistemi üzerine inhibitör etkileri C. violaceum, 026, C. violaceum VIR07, Pseudomonas aeruginosa PAO1, P. aeruginosa PAC-1 kullanılarak araştırılmıştır. Sonuçlar alfa pinen, terpineol, sineol, linelol ve kamforun C. violaceum, 026, C. violaceum VIR07 suşunda viyolasin üretiminin hem de, P. aeruginosa PAO1 ve P. aeruginosa PAC-1 klinik izolatında piyosiyanin, elastaz üretimi ve kayma hareketini önemli derecede inhibe ettiğini göstermiştir. Çalışmanın sonuçları alfa pinen, terpieneol, sineol, linelol ve kamforun, C violaceum ve P. aeruginosa için hücreler arası iletişim sistemi üzerine inhibitör etkileri bulunduğu tespit edilmiştir.

  7. El ácido alfa lipoico y su poder antioxidante frente al cáncer y las patologías de sensibilización central

    OpenAIRE

    Durand, Marisa

    2013-01-01

    El objetivo del presente trabajo es reunir y contrastar información sobre las propiedades antioxidantes del ácido alfa lipoico (ALA) en la prevención y desarrollo de las patologías relacionadas con el estrés oxidativo. L'objectiu del present treball és reunir i contrastar informació sobre les propietats antioxidants de l'àcid alfa lipoico (ALA) en la prevenció i desenvolupament de les patologies relacionades amb l'estrès oxidatiu.

  8. Informe sobre el Proyecto Alfa Biblioteca de Babel: ¿Un atajo para la administración de bibliotecas universitarias? Biblioteca de Babel Alfa Project Report: A shortcut for the academic libraries management?

    Directory of Open Access Journals (Sweden)

    Liliana Laura Rega

    2006-12-01

    Full Text Available El Proyecto Alfa Biblioteca de Babel que reúne bibliotecas universitarias de América Latina y Europa comenzó en marzo de 2005 y prevé su conclusión en marzo de 2007. El presente trabajo intenta describir los objetivos y los resultados esperados, e informa las actividades realizadas por la Red Biblioteca de Babel. Finalmente se analizan las propuestas del proyecto en cuanto al rol de las bibliotecas universitarias, y su relación con la innovación en las prácticas pedagógicas.Biblioteca de Babel Alfa Project that assembles academic libraries from Latin America and Europe was approved on March, 2005 and its conclusion is foreseen on March, 2007. This article attempts to describe the aims and the expected results, and reports the activities of the Biblioteca de Babel Network. Finally it analizes the proposals of the project about the role of academic libraries and their relationship with innovations in pedagogical practices.

  9. Treatment of severe sepsis: where next? Current and future treatment approaches after the introduction of drotrecogin alfa

    Directory of Open Access Journals (Sweden)

    Todd W Rice

    2006-03-01

    Full Text Available Todd W RiceDivision of Allergy, Pulmonary, and Critical Care Medicine, Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN, USAAbstract: Severely septic patients continue to experience excessive morbidity and mortality despite recent advances in critical care. Although significant resources have been invested in new treatments, almost all have failed to improve outcomes. An improved understanding of sepsis pathophysiology, including the complex interactions between inflammatory, coagulation, and fibrinolytic systems, has accelerated the development of novel treatments. Recombinant human activated protein C (rhAPC, or drotrecogin alfa (activated (DAA, is currently the only US Food and Drug Administration (FDA-approved medicine for the treatment of severe sepsis, and only in patients with a high risk of death. This review will discuss the treatment of severe sepsis, focusing on recent discoveries and unresolved questions about DAA’s optimal use. Increasing pharmacological experience has generated enthusiasm for investigating medicines already approved for other indications as treatments for severe sepsis. Replacement doses of hydrocortisone and vasopressin may reduce mortality and improve hypotension, respectively, in a subgroup of patients with catecholamine-refractory septic shock. In addition to discussing these new indications, this review will detail the provocative preliminary data from four promising treatments, including two novel modalities: antagonizing high mobility group box protein and inhibiting tissue factor (TF. Observational data from the uncontrolled administration of heparin or statins in septic patients will also be reviewed.Keywords: septic shock; drotrecogin alfa, vasopressin, new therapies, statins, high mobility group box protein

  10. Alfa247®: Un modelo de aprendizaje diferenciado para la enseñanza del Nordic Walking

    Directory of Open Access Journals (Sweden)

    Rodolfo Iván Martínez Lemos

    2010-03-01

    Full Text Available La técnica alfa y el método de los 7 pasos constituyen un modelo de aprendizaje diferenciado para la enseñanza del Nordic Walking (NW. NW consiste en caminar con bastones especialmente diseñados y utilizando una técnica precisa que moviliza una gran parte de la masa muscular total y tiene efectos beneficiosos probados sobre la salud. Alfa247® hace posible que cualquier persona, con independencia de su nivel de habilidad o condición física, pueda familiarizarse y aprender la técnica de caminar con bastones. Este nuevo concepto de movimiento saludable está todavía dando sus primeros pasos en España y es probable que en un futuro próximo pueda jugar un papel importante dentro del currículo de la Educación Física Escolar, como ya ha hecho en otros países del entorno.

  11. Factor de necrosis tumoral alfa en una población infanto-juvenil con sobrepeso

    Directory of Open Access Journals (Sweden)

    Teresita del R. Carrizo

    2013-08-01

    Full Text Available El sobrepeso infantil está asociado a sobrepeso/obesidad en la edad adulta. El tejido adiposo en obesos produce una cantidad incrementada de citoquinas proinflamatorias como el factor de necrosis tumoral alfa (TNF-a, ejerciendo un efecto deletéreo sobre la función vascular. El objetivo de este trabajo fue evaluar niveles de TNF-a en una población infantojuvenil con sobrepeso y su relación con otras variables. Se estudiaron 30 niños con sobrepeso (12 varones de edades entre 8-13 años, se midió circunferencia de cintura (CC e índice de masa corporal (IMC y fueron comparados con 20 controles de edad y sexo semejantes. Se consideró criterio de inclusión un IMC = 85 < 95 percentilo para edad y sexo. En ambos grupos se determinó: glucemia en ayunas (método glucosa oxidasa, insulina plasmática (ECLIA, fibrinógeno (Fg, método de Clauss, proteína C reactiva ultrasensible (uPCR, método inmunoturbidimétrico, TNF-a (ELISA, perfil lipídico (métodos enzimáticos, eritrosedimentación y se calculó el índice HOMA. Los datos se expresaron como mediana y rango intercuartil y con el coeficiente de Spearman se investigaron las correlaciones entre variables, considerándose significativo un p < 0.05. Los niveles de TNF-a fueron mayores en los sujetos con sobrepeso [15.4 (13.2-24.0 vs. 12.7 (11.2-14.8 pg/ml; p = 0.028]. También resultaron más elevados los valores de Fg, insulina plasmática, índice HOMA, uPCR y triglicéridos. El TNF-a se correlacionó con la CC (r = 0.654; p = 0.021. Los niveles elevados de TNF-a, uPCR y Fg encontrados confirman un estado proinflamatorio asociado a obesidad abdominal en la población estudiada.

  12. Single-arm, observational study of the ease of use of a redesigned pen device to deliver recombinant human follicle-stimulating hormone (follitropin alfa for assisted reproductive technology treatment

    Directory of Open Access Journals (Sweden)

    Illingworth PJ

    2014-06-01

    Full Text Available Peter J Illingworth,1 Robert Lahoud,1 Frank Quinn,1 Kendal Chidwick,2 Claire Wilkinson,2 Gavin Sacks1 1IVFAustralia, Greenwich, Sydney, NSW, Australia; 2Scientific Affairs, Merck Serono Australia Pty Ltd, Frenchs Forest, Sydney, NSW, Australia Purpose: Evaluation of patients’ ease of use of the redesigned, disposable, ready-to-use ­follitropin alfa pen during controlled ovarian stimulation for assisted reproductive technology. Methods: This single-center, observational, open-label, single-arm study recruited infertile normo-ovulatory women (aged 18–45 years. Nurses trained patients to self-administer recombinant human follicle-stimulating hormone daily using the follitropin alfa pen (300 IU, 450 IU, and 900 IU. Before treatment, patients completed Questionnaire A. Following self-administered treatment, on stimulation days 5–6 and 7–8 (within a day of receiving recombinant human chorionic gonadotropin, patients completed Questionnaire B. Nurses completed an ease-of-learning/teaching questionnaire. The primary endpoint was proportion of patients rating the pen as “easy/very easy” to use (Questionnaire B on the final visit before recombinant human chorionic gonadotropin. Secondary endpoints included: proportion of patients rating the follitropin alfa pen as easy to learn, use, prepare, deliver, and dispose of (Questionnaires A and B. Proportions (95% confidence intervals [CIs] were provided for primary and secondary endpoints. Adverse events were reported descriptively. Results: Eighty-six patients received recombinant human follicle-stimulating hormone. Of the 72 patients who had completed the overall assessment questions, 66 (91.7%; 95% CI =82.7%–96.9% found the pen “easy” to use. Also, 70/86 (81.4% patients “strongly agreed/agreed” that, overall, it was easy to learn how to use the pen; 72/86 (83.7% “strongly agreed/agreed” that easily understandable, verbal information was provided; and 70/86

  13. Prospective, randomized, controlled, and open study in primarily inoperable, stage III non-small cell lung cancer (NSCLC) patients given sequential radiochemotherapy with or without epoetin alfa

    International Nuclear Information System (INIS)

    Background and purpose: Induction chemotherapy is associated with anemia in non-small cell lung cancer (NSCLC) patients undergoing radiotherapy. This randomized, open-label study compared the effect of sequential radiochemotherapy (RCHT) versus RCHT + epoetin alfa (RCHT + EPO), with respect to 2-year overall survival (OS). Material and methods: Patients ⩾ 18 years received sequential RCHT; one arm also received EPO (chemotherapy day 1, when Hb < 12 g/dL). Kaplan–Meier analysis with log-rank test, and Cox-regression methods were performed. Results: Of the 385 patients randomized (RCHT + EPO: n = 195; RCHT: n = 190), 78 (RCTH + EPO: 46 [23.6%]; RCHT: 32 [16.8%]) were anemic at baseline. Two-year OS was higher in RCHT + EPO-treated versus RCHT-treated (28.5% [95% CI: 22.2–35.1%] versus 20.6% [95% CI: 15.1–26.8%] [p = 0.2278]), and requirement for RBC transfusion was lower (24/195 [12.3%] versus 61/190 [32.1%]). In anemic (baseline) patients (post hoc analysis), median survival was shorter in RCTH-treated (212 days) versus RCHT + EPO-treated (343 days) (Hazard ratio = 1.62 [95% CI: 0.99–2.63], p = 0.0525). Adverse events were documented in 72.7% (RCHT + EPO: 75.0%; RCHT: 70.5%) patients, and thrombovascular events (TVEs) in 45 patients (RCHT + EPO: 16.7%; RCHT: 7.9%; p = 0.0099). Conclusions: A statistically non-significant trend for 2-year OS was observed in a sub-group of EPO-treated NSCLC-patients with baseline anemia, although this trend was not maintained in the overall population with inoperable NSCLC

  14. Alfa Laval to meet the ballast water challenge%阿法拉伐应对压载水的挑战

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    Alfa Laval, the market leader in separation, heat transfer and freshwater generation, is widening its offering of solutions for environmental protection. The company hereby announces that a potent technology for dealing with unwanted organisms in ballast water will be commercially available as early as 2006.

  15. Human recombinant interferon alfa-2a for the treatment of Behçet's disease with sight threatening posterior or panuveitis

    OpenAIRE

    Kötter, I.; Zierhut, M.; Eckstein, A K; Vonthein, R; Ness, T; Günaydin, I; Grimbacher, B.; Blaschke, S; Meyer-Riemann, W; Peter, H H; Stübiger, N

    2003-01-01

    Background: Behçet's disease is a multisystem vasculitis of unknown origin. Standard treatment mainly comprises systemic immunosuppressive agents. Ocular involvement, mostly posterior uveitis with retinal vasculitis, leads to blindness in 20–50% of the involved eyes within 5 years. The efficacy of interferon alfa-2a was studied in patients with sight threatening posterior uveitis or retinal vasculitis.

  16. Post-surgery adjuvant therapy with intermediate doses of interferon alfa 2b versus observation in patients with stage IIb/III melanoma (EORTC 18952): randomised controlled trial.

    NARCIS (Netherlands)

    Eggermont, A.M.M.; Suciu, S.; MacKie, R.; Ruka, W.; Testori, A.; Kruit, W.; Punt, C.J.A.; Delauney, M.; Sales, F.; Groenewegen, G.; Ruiter, D.J.; Jagiello, I.; Stoitchkov, K.; Keilholz, U.; Lienard, D.

    2005-01-01

    BACKGROUND: Individuals affected by melanoma with thick primary tumours or regional node involvement have a poor outlook, with only 30-50% alive at 5 years. High-dose and low-dose interferon alfa have been assessed for the treatment of these patients, with the former having considerable toxicity and

  17. Long-term outcome of hepatitis B e antigen-positive patients with compensated cirrhosis treated with interferon alfa. European Concerted Action on Viral Hepatitis (EUROHEP)

    DEFF Research Database (Denmark)

    Fattovich, G; Giustina, G; Realdi, G;

    1997-01-01

    The aim of this study was to evaluate whether interferon alfa (IFN-alpha) treatment-associated virological and biochemical remission improves survival in a cohort of 90 white patients with compensated cirrhosis caused by hepatitis B (Child A) followed for a mean period of 7 years. Inclusion...

  18. Effectiveness of interferon alfa on incidence of hepatocellular carcinoma and decompensation in cirrhosis type C. European Concerted Action on Viral Hepatitis (EUROHEP)

    DEFF Research Database (Denmark)

    Fattovich, G; Giustina, G; Degos, F;

    1997-01-01

    The role of interferon alfa treatment in improving morbidity endpoints in patients with chronic hepatitis C infection is currently under debate. The aim of this study was to evaluate the effectiveness of interferon in preventing hepatocellular carcinoma and decompensation in cirrhosis type C....

  19. Urinary and fecal excretion of 17 alfa-methyltestosterone by veal calves injected with an anabolic combination of methyltestosterone and estradiol benzoate

    NARCIS (Netherlands)

    Jansen EHJM; van den Berg RH; Enkelaar-Willemsen C; Both-Miedema R; Zomer G; Stephany RW

    1986-01-01

    De uitscheiding van 17 alfa-methyltestosteron (MT) in urine en faeces is onderzocht bij twee mannelijke vleeskalveren die ingespoten zijn met een combinatie van methyltestosteron en 17 beta-estradiol-3-benzoaat opgelost in olie. Om mogelijke metabolieten op te sporen zijn immunogrammen gemaakt met

  20. Co-infection of SENV-D among chronic hepatitis C patients treated with combination therapy with high-dose interferon-alfa and ribavirin

    OpenAIRE

    Dai, Chia-Yen; Chuang, Wan-Long; Chang, Wen-Yu; Chen, Shinn-Cherng; Lee, Li-Po; Hsieh, Ming-Yen; Hou, Nei-Jen; Lin, Zu-Yau; Hsieh, Ming-Yuh; Wang, Liang-Yen; Yu, Ming-Lung

    2005-01-01

    AIM: The clinical significance of co-infection of SENV-D among patients with chronic hepatitis C (CHC) and response of both viruses to combination therapy with high-dose interferon-alfa (IFN) plus ribavirin remain uncertain and are being investigated.

  1. Darbepoetin alfa for treating chemotherapy-induced anemia in patients with a baseline hemoglobin level < 10 g/dL versus ≥10 g/dL: an exploratory analysis from a randomized, double-blind, active-controlled trial

    International Nuclear Information System (INIS)

    Several studies have shown that darbepoetin alfa, an erythropoiesis-stimulating agent (ESA), can reduce transfusions and increase hemoglobin (Hb) levels in patients with chemotherapy-induced anemia (CIA). Recent safety concerns, however, have prompted changes to ESA product information. In the European Union and United States, ESA therapy initiation for CIA is now recommended at a Hb level ≤10 g/dL. The present exploratory analysis examined how ESA initiation at this Hb level may impact patient care. Data from a phase 3 randomized trial were retrospectively reanalyzed. CIA patients with nonmyeloid malignancies were randomized 1:1 to 500 mcg darbepoetin alfa every three weeks (Q3W) or 2.25 mcg/kg darbepoetin alfa weekly (QW) for 15 weeks. A previously published report from this trial showed Q3W dosing was non-inferior to QW dosing for reducing transfusions from week 5 to end-of-the-treatment period (EOTP). In the present analysis, outcomes were reanalyzed by baseline Hb <10 g/dL and ≥10 g/dL. Endpoints included transfusion rates, Hb outcomes, and safety profiles. This study reanalyzed 351 and 354 patients who initiated ESA therapy at a baseline Hb of <10 g/dL or ≥10 g/dL, respectively. From week 5 to EOTP, the estimated Kaplan-Meier transfusion incidence (Q3W vs QW) was lower in the ≥10 g/dL baseline-Hb group (14% vs 21%) compared with the <10 g/dL baseline-Hb group (36% vs 41%). By week 5, the ≥10 g/dL baseline-Hb group, but not the <10 g/dL baseline-Hb group, achieved a mean Hb ≥11 g/dL. The Kaplan-Meier estimate of percentage of patients (Q3W vs QW) who achieved Hb ≥11 g/dL from week 1 to EOTP was 90% vs 85% in the ≥10 g/dL baseline-Hb group and 54% vs 57% in the <10 g/dL baseline-Hb group. Both baseline-Hb groups maintained mean Hb levels <12 g/dL and had similar safety profiles, though more patients in the ≥10 g/dL baseline-Hb group reached the threshold Hb of ≥13 g/dL. In this exploratory analysis, darbepoetin alfa Q3W and QW raised Hb

  2. Efeitos do alfa-tocoferol nas lesões de isquemia-reperfusão induzidas em pata direita de ratos

    Directory of Open Access Journals (Sweden)

    Bitu-Moreno José

    2001-01-01

    Full Text Available Recentes estudos demonstraram que os radicais livres de oxigênio parecem desempenhar papel importante nas lesões de isquemia-reperfusão. O alfa-tocoferol(AT age in vivo como um anti-oxidante. Nosso objetivo foi o de verificar se o AT atenuava as lesões de isquemia-reperfusão em pata direita de ratos. Os animais, randomizados, foram divididos nos seguintes grupos(G: G1-controle sem isquemia; G2 e G3 - quatro horas isquemia e duas reperfusão. Os animais do G2 foram tratados com salina e os do G3 com AT, 50mg/kg. Como parâmetros examinamos as alterações de volume e circunferência do pé direito ao longo do experimento, assim como a captação de 99mTc-pirofosfato e a microscopia eletrônica de transmissão do músculo soleus direito. Os aumentos de volume e circunferência do pé dos animais do G2 foram estatisticamente maiores quando comparados com o G1. As medidas do G3 não diferiram do G1. A captação de 99mTc-pirofosfato foi bem aumentada nos G2 e G3, quando comparados ao G1, porém sem diferenças entre os grupos isquêmicos. As lesões ao MET foram menos intensas em três animais do G3, quando comparados ao G2. Conclusão: O tratamento com AT diminuiu a formação do edema mas só protegeu parcialmente as células musculares contra as lesões de isquemia-reperfusão.

  3. El receptor de estrógenos alfa como mediador del efecto proliferativo de progestágenos en cáncer de mama

    Directory of Open Access Journals (Sweden)

    Sebastián Giulianelli

    2012-08-01

    Full Text Available En carcinomas mamarios murinos (C4-HD y en células de cáncer de mama humano (T47D observamos que el progestágeno sintético, acetato de medroxiprogesterona (MPA, induce la activación del receptor de estrógenos alfa (REa y su asociación nuclear con el receptor de progesterona (RP. En este trabajo postulamos que dicha interacción a nivel genómico sería fundamental para desarrollar respuestas proliferativas mediadas por progestágenos. Demostramos que el antiestrógeno fulvestrant (FUL, ICI182.780 indujo la regresión completa de tumores C4-HD creciendo con MPA. El progestágeno indujo la expresión temprana de CCND1 y MYC en células T47D y este efecto fue revertido al bloquear el REa. En células tratadas con MPA utilizamos ensayos de inmunoprecipitación de la cromatina (ChIP y corroboramos la colocalización nuclear de RP/REa en los mismos sitios de los promotores de CCND1 y MYC. El ICI no afectó la unión de RP a ambas secuencias regulatorias, pero sí inhibió la unión del REa. Confirmamos la interacción nuclear entre REa y RP en muestras de cáncer de mama humano. Los resultados demuestran que la presencia del REa, interactuando con el RP, en promotores de CCND1 y MYC es fundamental para la transcripción génica y la proliferación celular inducida por el progestágeno.

  4. 长效干扰素联合利巴韦林治疗慢性丙型肝炎疗效观察%Study on peginterferon alfa-2a combined with ribavirin in patients with chronic hepatitis C

    Institute of Scientific and Technical Information of China (English)

    杨艳杰; 王福; 郭艳玲; 齐欣

    2011-01-01

    Objective It is to investigate the effect and safety of peginterferon alfa - 2a combined with ribavirin in patients with chronic hepatitis C. Methods Fifty-six patients with chronic hepalitis C were randomly divided into test group ( n = 29 )and control group ( n = 27 ). Patients in control group were assigned interferon alfa -2a plus ribavirin, and in test group were administered peginterferon alfa -2a plus ribavirin. Effect was assessed by measuring hepaiitis C virus ( HCV ) RNA before treatment. and for 48 weeks, and following a 24-week treatment-free period. Results The overall response rates ( VR ) in test group were significantly higher than control group at 48 weeks and following a 24-week treatment-free period ( both P < 0.01 ).Comparison between different genotypes, there were significant differences only in complete response between after 48 weeks of treatment and follow-up time of 24 weeks in HCV genotype 1 infected patients ( P < 0.01 ). There was no significant difference among the other groups ( P >0.05 ). The RNA quantitative level of HCV had no significant impact on the virological complete response in control group and test group ( P > 0.05 ). The major side effects included flu-like syndrome, anemia, neutropenia and thrombocytopenia, but which did not affect the clinical use of drugs. Conclusion For patients with chronic hepatitis C . the comhination of peginterferon alfa - 2a plus ribavirin is the most effective therapy.%目的 评价长效干扰素(Peg-IFN-α-2a) 联合利巴韦林治疗慢性丙型肝炎(CHC)的临床疗效及安全性.方法 56例慢性丙型肝炎患者随机被分为试验组(n=29)和对照组(n=27).其中试验组给予Peg-IFN-α-2a联合利巴韦林治疗;对照组给予IFN-α-2a联合利巴韦林治疗.治疗时间均为48周,随访时间24周.分别检测治疗前、用药48周及停药24 周的病毒学、生化学、基因分型,比较2组间治疗结果以及ALT、HCV RNA 定量及

  5. Systemic lupus erythematosus following virological response to peginterferon alfa-2b in a transplanted patient with chronic hepatitis C recurrence

    Institute of Scientific and Technical Information of China (English)

    Francesca Lodato; Giuseppe Mazzella; Maria Rosa Tamé; Antonio Colecchia; Chiara Racchini; Francesco Azzaroli; Antonia D'Errico; Silvia Casanova; Antonio Pinna; Enrico Roda

    2006-01-01

    Autoimmune manifestations are common both in patients chronically infected by hepatitis C virus, and in patients transplanted for non-autoimmune diseases. A correlation between interferon based treatment and autoimmune diseases or the development of autoantibodies is well established in non-transplanted patients, but few data are available about transplanted patients. It is unclear whether interferon may increase the incidence of acute cellular rejection and there are few reports on the development of atypical autoimmune manifestations during post-liver transplantation interferon or pegylated interferon treatment. We describe a case of systemic lupus erythematosus following treatment with pegylated interferon alfa-2b in a transplanted patient with recurrence of chronic hepatitis C. Our experience suggest that pegylated interferon may induce autoimmune diseases in the immunosuppressed host, different from acute cellular rejection and call for a great attention to possible autoimmune disorders development during interferon based treatments in liver transplanted patients.

  6. Alfapeguinterferon-2a e ribavirina versus alfapeguinterferon-2b e ribavirina: avaliação custo-efetividade e do impacto orçamentário do tratamento do genótipo 1 da hepatite C crônica Alfa-pegylated interferons (2a and 2b and ribavirin for treatment chronic hepatitis C, genotype 1: a cost-effectiveness analysis

    Directory of Open Access Journals (Sweden)

    Gabriela Bittencourt Gonzalez Mosegui

    2011-01-01

    Full Text Available Foi realizada análise de custo-efetividade e do impacto orçamentário de tratamentos indicados para adultos infectados com genótipo 1 do vírus da hepatite C, comparando o não-tratamento com terapias combinadas de alfapeguinterferon-2a e 2b associados a ribavirina. O modelo de Markov desenvolvido projetou a evolução da hepatite C em coorte de 1.000 pacientes, por um período de 30 anos, para os vários estados do desenvolvimento da doença. As terapias combinadas da ribavirina com o alfapeguinterferon 2a ou 2b apresentam efetividades estatisticamente idênticas quando avaliadas em 30 anos de evolução da doença. Quanto ao impacto do tratamento nos anos de vida e nos anos de vida ajustados por qualidade de vida; os anos de vida ganhos por qualidade de vida em relação à evolução da doença sem tratamento foram de 1,67 e 1,63, respectivamente, para a terapia combinada com alfapeguinterferon 2a e 2b, aplicando-se 5% de desconto. A estratégia de tratamento com alfapeguinterferon 2a mais ribavirina se revelou mais custo-efetiva, dominando a outra alternativa de tratamento. Embora não haja diferenças significativas de efetividade entre os dois tipos de alfapeguinterferon, a diferença de preço entre os dois medicamentos faz com que a alternativa do uso do alfapeguinterferon 2a mais ribavirina seja mais eficiente. Quanto à estimativa do impacto orçamentário para o período de 2008 a 2017, a utilização do alfapeguinterferon 2a mais ribavirina resulta em redução nos gastos de aproximadamente 19%, caso todos os doentes fossem tratados utilizando-se os esquemas terapêuticos selecionados.A cost-effectiveness and budget impact of treatments given to adults infected with genotype 1 hepatitis C virus was performed by comparing the non-treatment with combined therapy alfapeguinterferon-2a and 2b and ribavirin. The Markov model developed engineered the development of hepatitis C in a cohort of 1,000 patients for a period of 30 years for

  7. Successful Management of Enzyme Replacement Therapy in Related Fabry Disease Patients with Severe Adverse Events by Switching from Agalsidase Beta (Fabrazyme®) to Agalsidase Alfa (Replagal®)

    OpenAIRE

    Tsuboi, Kazuya; Yamamoto, Hiroshi; Somura, Fuji; Goto, Hiromi

    2014-01-01

    Background: Enzyme replacement therapy (ERT) is the only approved therapy for Fabry disease. In June 2009, there was a worldwide shortage of agalsidase beta, necessitating dose reductions or switching to agalsidase alfa in some patients.

  8. Health-related quality of life in patients with high-risk melanoma randomised in the Nordic phase 3 trial with adjuvant intermediate-dose interferon alfa-2b

    DEFF Research Database (Denmark)

    Brandberg, Y; Aamdal, S; Bastholt, Lars;

    2012-01-01

    To compare health-related quality of life (HRQoL) and side-effects in patients with high-risk melanoma participating in a randomised phase III trial of adjuvant interferon alfa-2b (IFN).......To compare health-related quality of life (HRQoL) and side-effects in patients with high-risk melanoma participating in a randomised phase III trial of adjuvant interferon alfa-2b (IFN)....

  9. Rat hepatocyte invasion by Listeria monocytogenes and analysis of TNF-alpha role in apoptosis Invasão de hepatócitos de rato por Listeria monocytogenes e análise do papel do TNF-alfa na apoptose

    Directory of Open Access Journals (Sweden)

    Sânia Alves dos Santos

    2005-04-01

    do hospedeiro e manipulação do citoesqueleto, resultando em morte celular. As interações entre células do hospedeiro e bactérias podem resultar em produção de citocinas como o Fator de Necrose Tumoral alfa (TNF-alfa. Hepatócitos têm potencial de produzir citocinas pro-inflamatórias como TNF-alfa, quando invadidos por bactérias. No presente trabalho demonstramos o comportamento dos hepatócitos invadidos por L. monocytogenes pela análise microscópica, determinação da produção de TNF-alfa por bioensaio e análise da apoptose pela técnica TUNEL. A presença da bactéria, na razão que variou de 5 a 50.000 bactérias por célula, induziu ruptura das monocamadas celulares. Observamos presença de bactérias internalizadas na 1ª hora de incubação por microscopia eletrônica. Os níveis de TNF-alfa aumentaram da 1ª hora de incubação até a 6ª hora, variando de 0 a 3749 pg/mL. Nas 7ª e 8ª horas de incubação, ocorreram quedas não significativas dos níveis de TNF-alfa, indicando possível saturação dos receptores celulares. A quantidade de TNF-alfa produzido por hepatócitos foi dependente do tempo de incubação, assim como da proporção entre bactérias e células. A taxa de apoptose aumentou diretamente com o tempo de incubação (1 h a 8 + 24 h, variando de 0 a 43%, assim como com a razão bactérias : células. Estes resultados mostram a habilidade de L. monocytogenes não-hemolítica em invadir os hepatócitos, e as principais conseqüências deste fenômeno são: liberação de TNF-alfa e indução de apoptose. Assim, podemos especular que hepatócitos usam apoptose induzida por TNF-alfa para liberar bactérias de seu interior, facilitando a destruição destas pelo sistema imune.

  10. Informe sobre el Proyecto Alfa Biblioteca de Babel: ¿Un atajo para la administración de bibliotecas universitarias?

    OpenAIRE

    Rega, Liliana Laura

    2006-01-01

    Biblioteca de Babel Alfa Project that assembles academic libraries from Latin America and Europe was approved on March, 2005 and its conclusion is foreseen on March, 2007. This article attempts to describe the aims and the expected results, and reports the activities of the Biblioteca de Babel Network. Finally it analizes the proposals of the project about the role of academic libraries and their relationship with innovations in pedagogical practices

  11. Recombinant Factor VIIa (Eptacog Alfa): A Pharmacoeconomic Review of its Use in Haemophilia in Patients with Inhibitors to Clotting Factors VIII or IX

    OpenAIRE

    Katherine A. Lyseng-Williamson; Plosker, Greg L.

    2007-01-01

    Recombinant factor VIIa (NovoSeven(R); also known as recombinant activated factor VII or eptacog alfa) is indicated as an intravenous haemostatic agent in haemophilia patients with inhibitors to clotting factors VIII or IX. In noncomparative trials in haemophilia patients with inhibitors, on-demand home treatment with recombinant factor VIIa was effective in controlling episodes of mild to moderate bleeding and well tolerated, with early treatment being associated with a greater rate of succe...

  12. Diffusion Kurtosis Imaging Detects Microstructural Alterations in Brain of alfa-Synuclein Overexpressing Transgenic Mouse Model of Parkinson’s Disease: A Pilot Study

    Czech Academy of Sciences Publication Activity Database

    Khainar, A.; Latta, P.; Dražanová, Eva; Rudá-Kučerová, J.; Szabó, N.; Arab, A.; Hutter-Paier, B.; Havas, D.; Windisch, M.; Šulcová, A.; Starčuk jr., Zenon; Rektorová, I.

    2015-01-01

    Roč. 28, č. 4 (2015), 281-289. ISSN 1029-8428 R&D Projects: GA MŠk(CZ) LO1212; GA MŠk ED0017/01/01 Institutional support: RVO:68081731 Keywords : diffusion kurtosis imaging * alfa-Synuclein * TNWT-61 * Parkinson’s disease * Transgenic mice * TBSS Subject RIV: BH - Optics, Masers, Lasers Impact factor: 3.538, year: 2014

  13. Co-infection of SENV-D among chronic hepatitis C patients treated with combination therapy with high-dose interferon-alfa and ribavirin

    Institute of Scientific and Technical Information of China (English)

    Chia-Yen Dai; Liang-Yen Wang; Ming-Lung Yu; Wan-Long Chuang; Wen-Yu Chang; Shinn-Cherng Chen; Li-Po Lee; Ming-Yen Hsieh; Nei-Jen Hou; Zu-Yau Lin; Ming-Yuh Hsieh

    2005-01-01

    AIM: The clinical significance of co-infection of SENV-D among patients with chronic hepatitis C (CHC) and response of both viruses to combination therapy with high-dose interferon-alfa (IFN) plus ribavirin remain uncertain and are being investigated.METHODS: Total 164 (97 males and 67 females, the mean age 48.1±11.4 years, range: 20-73 years, 128histologically proved) naive CHC patients were enrolled in this study. SENV-D DNA was tested by PCR method.Detection of serum HCV RNA was performed using a standardized automated qualitative RT-PCR assay (COBAS AMPLICOR HCV Test, version 2.0). HCV genotypes 1a,1b, 2a, 2b, and 3a were determined by using genotypespecific primers. Pretreatment HCV RNA levels were determined by using the branched DNA assay (Quantiplex HCV RNA 3.0). There are 156 patients receiving combination therapy with IFN 6 MU plus ribavirin for 24 wk and the response to therapy is determined.RESULTS: Sixty-one (37.2%) patients were positive for SENV-D DNA and had higher mean age than those who were negative (50.7±10.6 years vs46.6±11.6 years,P = 0.026). The rate of sustained viral response (SVR)for HCV and SENV-D were 67.3% (105/156) and 56.3%(27/48), respectively. By univariate analysis, the higher rate of SVR was significantly related to HCV genotype non-1b (P<0.001), younger ages (P = 0.014), lower pretreatment levels of HCV RNA (P = 0.019) and higher histological activity index (HAI) score for intralobular regeneration and focal necrosis (P = 0.037). By multivariate analyses, HCV genotype non-1b, younger age and lower pretreatment HCV RNA levels were significantly associated with HCV SVR (odds ratio (OR)/95% confidence interval (CI): 12.098/0.02-0.19, 0.936/0.890-0.998, and 3.131/1.080-9.077, respectively). The SVR of SENV-D was higher among patients clearing SENV-D than those who had viremia at the end of therapy (P = 0.04).CONCLUSION: Coexistent SENV-D infection, apparently associated with higher ages, is found in more than onethird Taiwanese

  14. Ocular changes due to systemic alpha-interferon therapy for hepatitis C Alterações oculares por uso sistêmico de alfa-interferon em pacientes com hepatite C

    Directory of Open Access Journals (Sweden)

    Liang Shih Jung

    2004-12-01

    Full Text Available PURPOSE: To describe ocular (specially fundoscopic changes due to systemic alpha-interferon (IFN therapy in patients with hepatitis C. This is a prospective, descriptive and observational (non-interventional study, and its results are preliminary. METHODS: Patients were selected with indication of alpha-interferon therapy for hepatitis C. They underwent ocular examinations before therapy and 1, 3, 6 and 12 months after starting the therapy. HIV patients and those who had a history of previous treatment with alpha-interferon were excluded from the study, but examined. RESULTS: Fifty-one patients were selected from November 1999 to June 2000. The male-to-female ratio was 1.55. The age average was 47 years. The best corrected visual acuity ranged from 20/15 to 20/40. Most of the symptomatic patients complained of foreign body sensation in the eyes, which became more tolerable with time. Complaints comprised flu-like symptoms, joint pain, depression, headache, and general weakness. Ocular findings related to alpha-interferon were: cotton wool spots (3 eyes and intraretinal hemorrhage (1 eye. One patient died during the study, due to another disease not related to hepatitis, and one patient refused to be examined. CONCLUSIONS: There are retinal vascular changes due to systemic alpha-interferon for hepatitis C. There are no studies in our country describing ocular changes in patients with hepatitis C on therapy with alpha-interferon. General physicians and specially gastroenterologists should pay special attention to this problem, and refer these patients to the ophthalmologist in order to determine and follow any lesion that may be related to the use of this drug.OBJETIVO: Avaliar alterações oculares (em especial lesões fundoscópicas em pacientes com hepatite C tratados com alfa-interferon (IFN. MÉTODOS: Estudo prospectivo, descritivo e observacional de pacientes com hepatite C do serviço de Gastroenterologia da UNIFESP com indicações de uso

  15. Cardiovascular effects of hemoglobin response in patients receiving epoetin alfa and oral iron in heart failure with a preserved ejection fraction

    Directory of Open Access Journals (Sweden)

    Sirish Vullaganti

    2014-06-01

    Full Text Available Background Previous data from a recently conducted prospective, single blind randomized clinical trial among community dwelling older patients with heart failure with a preserved ejection fraction (HFPEF and anemia randomized to treatment with epoetin alfa (erythro-poiesis-stimulating agents, ESA vs. placebo did not demonstrate significant benefits of therapy regarding left ventricular (LV structure, functional capacity, or quality of life (QOL. However, several patients randomized to the treatment arm were non-responders with a suboptimal increase in hemoglobin. All patients in the trial also received oral ferrous gluconate, which could have contributed to increases in hemoglobin observed in those receiving placebo. Accordingly, we performed an analysis separating patients into responders vs. non-responders in order to determine if measured improvement in anemia would have any effect on clinical endpoints. Methods A total of 56 patients (age 77 ± 11 years, 68% female were recruited who had anemia defined as a hemoglobin of ≤ 12 g/dL (average, 10.4 ± 1 g/dL with HFPEF defined as having NHANES-CHF (National Health And Nutrition Examination Survey: Congestive Heart Failure criteria score of ≥ 3 and an ejection fraction of > 40% (average EF = 63% ± 15%. Patients were randomly allocated to receive either ESA and ferrous gluconate or ferrous gluconate only. In this analysis, a responder was defined as a patient with an increase of 1 g/dL in the first 4 weeks of the trial. Results Nineteen subjects were classified as responders compared to 33 non-responders. While the average hemoglobin increased significantly at the end of 6 months for responders (1.8 ± 0.3 vs. 0.8 ± 0.2 g/dL, P = 0.004, 50% of the subjects assigned to ESA were non-responders. Left ventricular function including ejection fraction (P = 0.32 and end diastolic volume (P = 0.59 was unchanged in responders compared to non-responders. Responders also showed no significant

  16. Lichen Planus Induced by Pegylated Interferon Alfa-2a Therapy in a Patient Monitored for Delta Hepatitis

    Directory of Open Access Journals (Sweden)

    Safak Kaya

    2015-01-01

    Full Text Available Interferons are used for treatment of chronic hepatitis B. They can induce or exacerbate some skin disorders, such as lichen planus. In this study, as we know, we presented the first case developing lichen planus while receiving interferon treatment due to delta hepatitis. A 31-year-old male patient presented to our outpatient clinic with HBsAg positivity. With his analyses, HBV DNA was negative, anti-delta total was positive, ALT was 72 U/L (upper limit 41 U/L, and platelet was 119 000/mm3. He was therefore started on subcutaneous pegylated interferon alfa-2a therapy at 180 mcg/week for delta hepatitis. At month 4 of therapy, the patient developed diffuse eroded lace-like lesions in oral mucosa, white plaques on lips, and itchy papular lesions in the hands and feet. Lichen planus was considered by the dermatology clinic and topical treatment (mometasone furoate was given. The lesions persisted at month 5 of therapy and biopsy samples were obtained from oral mucosal lesions and interferon dose was reduced to 135 mcg/week. Biopsy demonstrated nonkeratinized stratified squamous epithelium; epithelial acanthosis, spongiosis, and apoptotic bodies were observed in the epidermis and therefore lichen planus was considered. At month 6 of therapy, lesions did not improve and even progressed and interferon treatment was therefore discontinued.

  17. Comparing effects of Beractant and Poractant alfa in decreasing mortality rate due to respiratory distress syndrome in premature infants

    Directory of Open Access Journals (Sweden)

    Saeidi R

    2011-02-01

    Full Text Available "nBackground: Exogenous natural and synthetic surfactants is a rescue treatment for respiratory distress syndrome (RDS. The goals of the study were to compare the clinical response and side-effects of two frequently used surfactants, poractant alfa (Curosurf and beractant (Survanta, for the treatment of respiratory distress syndrome in preterm infants."n "nMethods: This clinical trial study was performed during a two-year period in the Neonatal Intensive Care Unit of Ghaem Hospital in Mashhad, Iran. Sample size calculated by a 95% confidence and power of 80, included 104 premature neonates, 74 in survanta and 30 in curosurf groups. The level of statistical significance was considered to be < 0.05."n "nResults: There were no statistically significant differences between the infants treated by survanta or cursurf groups regarding their mean gestational age (30.58 Vs. 29.00 weeks and birth weight (1388 Vs. 1330 g, (p=0.3 There were also no significant differences between the two groups regarding incidences of broncho- pulmonary dysplasia (BPD (40.5% Vs. 40%, intraventricular hemorrhage (IVH grades III/IV (13.5% Vs. 13.3%, pneumothorax (both 20%, patent ductus arteriosus (PDA (28/3% Vs. 20% or death (28% Vs. 26.6% on the 28th day postpartum."n "nConclusion: This study showed that survanta and curosurf had similar therapeutic effects in the treatment of neonatal respiratory distress syndrome.

  18. The effectiveness of retreatment with peginterferon alfa and ribavirin in patients with chronic viral hepatitis C genotype 2 and 3: a prospective cohort study in Brazil

    Directory of Open Access Journals (Sweden)

    Artico Simara

    2012-12-01

    Full Text Available Abstract Background More than 50% of patients infected with chronic hepatitis C virus (HCV do not respond to treatment with conventional interferon (IFN combined with ribavirin (RBV. The aim of our study was to evaluate the effectiveness of retreatment with peginterferon alfa-2a or 2b (PEG-IFN 2a or 2b concomitantly with RBV in patients with HCV genotype 2 and 3, which were non-responders or relapsers to initial treatment with IFN / RBV and to identify possible predictors of sustained virological response (SVR. Methods From September 2003 to March 2009 a cohort of 216 patients who had previously failed therapy with a regimen of standard interferon and ribavirin, were followed in a specialized service implemented in the Brazilian Unified Health System, Rio Grande do Sul. All patients were retreated with PEG-IFN 2a or 2b per week, associated with RBV, through oral route, with doses determined according to weight (1,000 mg if weight ≤ 75 Kg and 1,250 mg if ≥ 75 Kg per day for 48 weeks. The HCV-RNA was tested by Polymerase Chain Reaction (PCR. Virological Response (VR within 48 weeks and SVR in the 72 weeks was considered for evaluation of treatment efficacy. Analyses were performed in patients who received at least one dose of PEG-IFN. Results The SVR rate for non-responders to previous treatment was 34.4% and for relapsers was 50% (p = 0.031. As predictive factors that contribute to improve SVR, were identified the age (p = 0.005, to be relapsers to previous treatment (p = 0.023 and present liver biopsy examination Metavir F0-F2 (p = 0.004. In assessing the safety profile, 51 patients (23.6% discontinued treatment prematurely. Conclusions This alternative retreatment for patients who have failed prior therapies for anti-HCV, has demonstrated promising SVR rate, provided that it includes a careful selection of patients with predictors of response and adverse events monitored.

  19. The Efficacy of Add-on Telbivudine Versus Switching to Pegylated Interferon Alfa-2a in Chronic Hepatitis B Patients With Poor Responses to Adefovir

    Directory of Open Access Journals (Sweden)

    Wei

    2016-01-01

    Full Text Available Background There are limited options for chronic hepatitis B (CHB patients who have poor responses to adefovir (ADV. Objectives The aim of this study is to evaluate the effects of adding on telbivudine (LdT or switching to pegylated interferon alfa-2a (PEG-IFN-α2a as alternative rescue therapies for patients with poor responses to the initial ADV treatments. Patients and Methods Ninety-seven CHB patients with HBV DNA > 2 log10 copies/mL 48 weeks after ADV monotherapy were included in this study. Fifty-nine of these patients were treated with a combination of LdT plus ADV (LdT + ADV daily, while thirty-eight patients were switched to PEG-IFN-α2a subcutaneous injections weekly for 48 weeks. Results Both rescue strategies were proven to be safe and the majority of patients tolerated the therapies well. LdT + ADV led to more rapid reductions in viral loads than PEG-IFN-α2a monotherapy, with 2.14 (LdT + ADV and 0.98 (PEG-IFN-α2a log10 copies/mL decreases 48 weeks after rescue treatments, respectively (P < 0.00001. The rates corresponding to virological and biochemical responses were also elevated in patients who received the LdT + ADV combination therapy at the end of the observation period (88.1 vs. 68.4% for virological response, P = 0.017; 83.3 vs. 47.2%, P = 0.00045. However, the decline in the hepatitis B surface antigen (HBsAg was more pronounced in PEG-IFN-α2a treated patients. Moreover, the cumulative rates of serological responses were higher in patients who switched to the PEG-IFN-α2a therapy. Conclusions Both add-on LdT and switching to PEG-IFN-α2a were satisfactory and optimal treatments for CHB patients with poor responses to ADV. Both rescue strategies resulted in significant reductions in serum viral load and ALT levels, and were associated with high rate of serological outcomes in our hospital.

  20. Intensive Farmaco- Surveillance of Interferon Alpha 2b Recombinant in Multiple Sclerosis. Farmacovigilancia intensiva al interferón alfa 2b recombinante en la esclerosis múltiple.

    Directory of Open Access Journals (Sweden)

    Hailen Bobillo López

    Full Text Available

    1pt 7.0865pt; text-align: justify;">Background: The interferon alpha 2b recombinant, produced in Cuba, is used in the treatment of different illnesses, such as the multiple sclerosis. For its commercialization it is needed to know its safety scope. Objective: To assess the adverse reactions of interferon alpha 2b recombinant in the treatment of multiple sclerosis. Method: During the period between 1996 and 2006, 70 clinical histories and data collection notebooks of patients included in the randomized double blind national clinical trial phase IV were revised. This trial was developed in the Clinic of Multiple Sclerosis of the Hospital "Dr. Gustavo Aldereguía Lima" in Cienfuegos. With regard to the total of manifested adverse reactions we analyzed type, length, and use of a given treatment to counteract them, intensity level (light, moderate, serious or lethal and causality level (definitive, probable, possible, conditional or not related. Results: 53 patients out of the total presented 207 adverse reactions to interferon. The most frequent were: fever, migraine, chills, arthralgia, asthenia and myalgia, being most of them moderate collateral effects of definitive character. In 197 patients the outcome was favorable. Conclusion: The use of the interferon alpha 2b

  1. Analysis of the Reports of Adverse Drug Reactions Induced by PEG-interferon Alfa-2a Injection%聚乙二醇干扰素α-2a注射液不良反应报告分析

    Institute of Scientific and Technical Information of China (English)

    韩丹; 代菲; 储文功

    2013-01-01

    目的:探讨聚乙二醇干扰素α-2a注射液不良反应发生的特点及相关因素,为临床安全用药提供参考.方法:收集某省药品不良反应监测中心2004~2011年自发呈报系统上报的1 606例聚乙二醇干扰素α-2a注射液的不良反应(ADR)报告,对ADR所涉及的患者年龄、性别,以及ADR年份、类型、结果、临床表现等进行统计分析.结果:1606例报告中,男883例,女723例,30 ~60岁患者最多(72.6%);发生严重不良反应243例,新的严重ADR 86例;临床表现以血液系统损害为主占59.95%,其次为肝胆系统损害占10.76%.结论:临床在运用聚乙二醇干扰素α-2a治疗丙肝的过程中,应密切观察其ADR,制定周密的护理计划,积极干预和防治,以有效减少和防止ADR发生.%To probe into the characteristics of adverse drug reactions (ADR) caused by PEG-interferon alfa-2a injection to provide some rational clinical guidance. Methods: 1606 reports of adverse reactions caused by PEG-interferon alfa-2a injection were collected from January 2004 to December 2011 and statistically analyzed in respect of patients'sex and age, involved type, years, result, clinical manifestations, etc. Results:883 of the 1606 cases were male cases,723 were female cases, and 72. 6% were the adults at the age of 30 to 60.The incidence rate of severe ADR were 243 cases, and new and severe ADR were 86 cases. The blood system was the main lesion in ADR cases (59.95%) , and the second was liver and gall system(10.76%). Conclusion: The corresponding adverse reactions should be closely observed during the clinical use of PEG-interferon alfa-2a in the treatment of hepatitis C. The occurrence of adverse reactions could be effectively reduced and prevented by making a careful nursing plan and conducting an active intervention and control.

  2. Retrospective US database analysis of drug utilization patterns, health care resource use, and costs associated with adjuvant interferon alfa-2b therapy for treatment of malignant melanoma following surgery

    Directory of Open Access Journals (Sweden)

    Krishna A

    2012-06-01

    Full Text Available Michelle D Hackshaw,1 Arun Krishna,2 David J Mauro31,2Global Health Outcomes, Merck, Sharpe and Dohme Corporation, Whitehouse Station, NJ, USA; 3Clinical Research, Merck Research Laboratories, Upper Gwynedd, PA, USABackground: The purpose of this study was to identify a real-world US population having undergone surgery for malignant melanoma and describe treatment patterns, health care resource utilization, and costs for patients who subsequently received interferon alfa-2b (IFN therapy or other standard of care chemotherapies.Methods: A retrospective cohort study was conducted using administrative claims from MarketScan® databases among melanoma patients diagnosed between 2004 and 2008 who had surgery and were subsequently treated with IFN or other chemotherapies. Health care resource utilization and costs of services (converted to 2009 dollars were evaluated. Cost refers to the amount paid to providers associated with the health service.Results: Of 18,075 subjects with melanoma surgery claims, 1525 (8.4% were treated with IFN and 1194 (6.6% with other chemotherapies. Median duration (days and number of doses of IFN therapy were 29 and 20, respectively. Approximately half of patients who received IFN discontinued therapy within or after the one-month induction phase. For IFN therapy patients, average total cost per patient for the last melanoma-related surgery prior to start of therapy, including costs of the surgery itself, pathology, anesthesia, and hospital care, was $2219. The average total cost per patient related to IFN therapy was $1188; this included costs for drug, office visits, blood work, and infusions. Other chemotherapy costs ranged from $146 to $2678.Conclusion: There is an unmet treatment need, considering that this study observed that melanoma patients on IFN therapy post-surgery do not complete the recommended one-year course of treatment which may compromise its full therapeutic benefits. Further study to investigate reasons

  3. Prediction of treatment week eight response & sustained virologic response in patients treated with boceprevir plus peginterferon alfa and ribavirin.

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    Alex Thompson

    Full Text Available AIM: Sustained virologic response (SVR can be attained with boceprevir plus peginterferon alfa and ribavirin (PR in up to 68% of patients, and short duration therapy is possible if plasma HCV RNA levels are undetectable at treatment week 8 (TW8 response. We have developed predictive models for SVR, and TW8 response using data from boceprevir clinical trials. METHODS: Regression models were built to predict TW8 response and SVR. Separate models were built for TW8 and SVR using baseline variables only, and compared to models with baseline variables plus HCV RNA change after 4 weeks of PR (TW4 delta. Predictive accuracy was assessed by c-statistics, calibration curves, and decision curve analyses. Nomograms were developed to create clinical decision support tools. Models were externally validated using independent data. RESULTS: The models that included TW4 delta produced the best discrimination ability. The predictive factors for TW8 response (n = 856 were TW4 delta, race, platelet count and ALT. The predictive factors for SVR (n = 522 were TW4 delta, HCV-subtype, gender, BMI, RBV dose and platelet count. The discrimination abilities of these models were excellent (C-statistics = 0.88, 0.80 respectively. Baseline models for TW8 response (n = 444 and SVR (n = 197 had weaker discrimination ability (C-statistic = 0.76, 0.69. External validation confirmed the predictive accuracy of the week 4 models. CONCLUSIONS: Models incorporating baseline and treatment week 4 data provide excellent prediction of TW8 response and SVR, and support the clinical utility of the lead-in phase of PR. The nomograms are suitable for point-of-care use to inform individual patient and physician decision-making.

  4. Hepatitis B surface antigen clearance in inactive hepatitis B surface antigen carriers treated with peginterferon alfa-2a

    Science.gov (United States)

    Li, Ming-Hui; Xie, Yao; Zhang, Lu; Lu, Yao; Shen, Ge; Wu, Shu-Ling; Chang, Min; Mu, Cai-Qin; Hu, Lei-Ping; Hua, Wen-Hao; Song, Shu-Jing; Zhang, Shu-Feng; Cheng, Jun; Xu, Dao-Zhen

    2016-01-01

    AIM: To examine the association between interferon (IFN) therapy and loss of hepatitis B surface antigen (HBsAg) in inactive HBsAg carriers. METHODS: This was a retrospective cohort study in inactive HBsAg carriers, who were treatment-naive, with a serum HBsAg level < 100 IU/mL and an undetectable hepatitis B virus (HBV) DNA level (< 100 IU/mL). All the 20 treated patients received subcutaneous PEG-IFN alfa-2a 180 μg/wk for 72 wk and were then followed for 24 wk. There were 40 untreated controls matched with 96 wk of observation. Serum HBsAg, HBV DNA, and alanine aminotransferases were monitored every 3 mo in the treatment group and every 3-6 mo in the control group. RESULTS: Thirteen (65.0%) of 20 treated patients achieved HBsAg loss, 12 of whom achieved HBsAg seroconversion. Mean HBsAg level in treated patients decreased to 6.69 ± 13.04 IU/mL after 24 wk of treatment from a baseline level of 26.22 ± 33.00 IU/mL. Serum HBV DNA level remained undetectable (< 100 IU/mL) in all treated patients during the study. HBsAg level of the control group decreased from 25.72 ± 25.58 IU/mL at baseline to 17.11 ± 21.62 IU/mL at week 96 (P = 0.108). In the control group, no patient experienced HBsAg loss/seroconversion, and two (5.0%) developed HBV reactivation. CONCLUSION: IFN treatment results in HBsAg loss and seroconversion in a considerable proportion of inactive HBsAg carriers with low HBsAg concentrations. PMID:27239256

  5. El receptor de estrógenos alfa como mediador del efecto proliferativo de progestágenos en cáncer de mama

    OpenAIRE

    2012-01-01

    En carcinomas mamarios murinos (C4-HD) y en células de cáncer de mama humano (T47D) observamos que el progestágeno sintético, acetato de medroxiprogesterona (MPA), induce la activación del receptor de estrógenos alfa (REa) y su asociación nuclear con el receptor de progesterona (RP). En este trabajo postulamos que dicha interacción a nivel genómico sería fundamental para desarrollar respuestas proliferativas mediadas por progestágenos. Demostramos que el antiestrógeno fulvestrant (FUL, ICI182...

  6. El ácido alfa lipoico y su poder antioxidante frente al cáncer y las patologías de sensibilización central

    OpenAIRE

    Marisa Durand; Núria Mach

    2013-01-01

    Introducción: El ácido alfa lipoico (ALA) puede controlar y limitar la cantidad de radicales libres, influyendo el desarrollo de patologías como el cáncer o las enfermedades de sensibilización central, aunque los mecanismos moleculares implicados en este proceso aún están dilucidándose. Objetivo: Reunir y contrastar información sobre las propiedades antioxidantes del ALA en la prevención y desarrollo de las patologías relacionadas con el estrés oxidativo. Material y métodos: En este trabajo, ...

  7. Retinopathy and subconjunctival haemorrhage in patients with chronic viral hepatitis receiving interferon alfa.

    OpenAIRE

    Hayasaka, S; Fujii, M.; Yamamoto, Y.; Noda, S; Kurome, H; Sasaki, M

    1995-01-01

    A total of 43 patients (86 eyes) with chronic viral hepatitis were examined prospectively before and after the start of interferon therapy. Of 37 non-diabetic patients, 23 (group A1) did not have retinopathy or subconjunctival haemorrhage, 11 (group A2) developed retinopathy, and three (group A3) exhibited subconjunctival haemorrhage during the treatment. In most eyes, the retinopathy disappeared after therapy was stopped. Of six diabetic patients, three (group B1) developed retinopathy and t...

  8. α-2-HS-糖蛋白对聚乙二醇干扰素alfa-2b治疗慢性乙型肝炎患者e抗原血清转换预测作用的研究%α-2-HS-glycoprotein:A Potential Marker Predicting Chronic Hepatitis B Patients E Seroconversion During Pegylated Interferon Alfa-2b By 4 week Up-regulated

    Institute of Scientific and Technical Information of China (English)

    马慧; 王江华; 郭芳; 魏来

    2010-01-01

    利用干扰素治疗慢性乙型肝炎仅在约1/3的患者中取得了满意的治疗效果.采用基于双向电泳的血清蛋白质组学技术,寻找慢性乙型肝炎患者干扰素治疗时发生e抗原血清学转换的预测标志物.对发生/未发生血清学转换组的患者基线和治疗24周血清进行差异蛋白的筛选和鉴定,并对筛选得到的a-2-HS-糖蛋白在训练组和验证组患者个体中进行了基线和第4周的进一步验证实验.结果发现,a-2-HS-糖蛋白从治疗基线至治疗第4周的变化趋势对慢性乙型肝炎患者干扰素alfa-2b治疗过程中e抗原血清学转换具有预测意义.

  9. EFEK PEMBERIAN ANTIOKSIDAN TOKOFEROL ALFA TERHADAP MEMBRAN SEL DARAH MERAH TALASEMIA

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    Ferry P Gultom

    2015-09-01

    Full Text Available Talasemia is blood disorder which is characterized by anemia due to inherited haemoglobine disorder. The anemia is caused by a decreased flexibility of the erythrocyte results in decreased capability of required deformability to be able to across through capillary blood vessels. Free radicals with one or more unpaired electron on potential in destroying all the molecule exist in the body of living things like protein, carbohydrate and fat. Tocoferol or vitamin E is an antioxidant essential in eliminating free radicals in the cell membrane by inhibiting the peroxidation process of the cell membrane lipid. The objective of this study is to investigate the severity of the erythrocyte destruction either those with or without oxidative load from the environment. The results of this study showed that the level of malondialdehid in the control of erythrocyte was 1.85 ± 1.37 nmol in talasemia and 1.02 ± 0.64 nmol in normal (p<0.05. The addition of 2mM t-BHP resulted in increased malondialdehid level up to 19.59 ± 6.82 nmol in talasemia and 11.42 ± 3.49 nmol in normal erythrocytes (P<0.05. Oxidated erythrocyte with vitamin E supplement beforehand showed lower level of malondialdehid composed to those without vitamin E supplement (p<0.05. Conclusion: Additional vitamin E (tocoferol leads to decreased level of malondialdehid both in the normal and thalasemia erythrocytes.

  10. 聚乙二醇干扰素α-2b致慢性丙型肝炎患者严重精神障碍%Mental disorder occuring in a patient with chronic hepatitis C receiving peginterferon alfa-2b

    Institute of Scientific and Technical Information of China (English)

    余俊玲; 冯爱桥; 张玉娟

    2011-01-01

    A 35-year-old male patient with chronic hepatitis C received IM peginterferon alfa-2b 2 million units once weekly. Four months later, the patient experienced auditory hallucination, delusion, and mild personality changes. Subsequently, his symptoms gradually aggravated. One week later, he presented with delusion of persecution, thought incoherence, emotion discord, irritability,and insight disorder. Peginterferon alfa-2b was withdrawn, and he received symptomatic treatment with olanzapine. One week after therapy, his symptoms improved and, two weeks after therapy, he was discharged, and continued taking olanzapine for one week, and then the drug was stopped by himself. The above-mentioned psychiatric symptoms did not recur during a half year of follow-up.%1例35岁男性患者,因慢性丙型肝炎给予聚乙二醇干扰素α-2b 200万U肌内注射,1次周.4个月后患者出现幻听、妄想,性格有轻微改变.随后症状逐渐加重,1周后出现被害妄想、思维散漫、情感不协调、易激惹和自知力障碍.遂停用该药,并给予奥氮平对症治疗.治疗1周症状明显好转,治疗2周出院.出院后继续应用奥氮平治疗1周,随后自行停药,随访半年未再出现上述精神症状.

  11. Elaboration, analyse et modélisation mécanique numérique d’agro-composites à base de fibres courtes d’alfa

    Directory of Open Access Journals (Sweden)

    El-Abbassi Fatima-ezzahra

    2014-04-01

    Full Text Available Dans la présente étude, récemment réalisée au Laboratoire d’Ingénierie et Sciences des Matériaux, de l’Université de Reims Champagne-Ardenne, nous proposons de valoriser la plante végétale d’Alfa, sous forme de fibres courtes en vue de renforcer par extrusion dans une première étape, puis par injection, des matrices polymères, en l’occurrence le Polypropylène (PP. Une première étape consiste à élaborer, par traitement chimique et extraction, la fibre courte à utiliser comme renfort. Il s’en suit une extrusion de courte à utiliser comme renfort. Il s’en suit une extrusion de composés composites à base de matrice PP. L’objectif sera de développer par la suite une démarche Essai-Calcul, confrontant une caractérisation mécanique d’agrocomposites PP-Alfa injectés à deux types de modélisation comportementale, l’une micromécanique basée sur le modèle de Mori-Tanaka, et l’autre numérique utilisant une approche originale basée sur une technique dite de fibre projetée.

  12. MICORRIZACIÓN In Vitro E In Vivo DE PLÁNTULAS DE PAPA (Solanum tuberosum var. Alfa

    Directory of Open Access Journals (Sweden)

    Kalyanne Fernández

    2010-01-01

    Full Text Available Las condiciones artificiales en las que se desarrollan las plantas obtenidas por micropropagación hacen que sean más susceptibles a la sequía durante el transplante. Los hongos micorrízicos arbusculares contribuyen a la supervivencia y el crecimiento de las plantas al reducir el estrés asociado con la nutrición, las relaciones con el agua, la estructura del suelo, el pH, las sales, los metales tóxicos y los patógenos, por lo que son considerados de extrema utilidad como inoculantes durante las diferentes fases de micropropagación de los cultivos. Teniendo en cuenta estos aspectos se realizó este trabajo, con el objetivo de evaluar la factibilidad de la inoculación micorrízica durante los estadios in vitro e in vivo del proceso de micropropagación del cultivo de la papa (Solanum tuberosum L var. Alfa. Para dar respuesta al trabajo, se estudió la inoculación micorrízica sobre plantas de papa en condiciones de crecimiento in vitro, empleando diferentes medios de cultivo (MS, M y un nuevo medio N, bajo un diseño completamente aleatorizado (DCA y la inoculación de plantas de papa durante el transplante a fase adaptativa empleando tres especies del género Glomus y dos concentrados de especies de HMA (selva y desierto sobre dos sustratos diferentes, utilizando un DCA bajo arreglo bifactorial. En el estudio in vitro se obtuvieron efectos positivos sobre las plantas inoculadas en el medio M, aun cuando quedó clara la necesidad de encontrar nuevos medios, nutricionalmente balanceados, que garanticen tanto el crecimiento de las plántulas como el establecimiento eficiente de la micorrización. Además, en la fase adaptativa se encontró una respuesta altamente positiva a la inoculación de las cepas, mostrando un comportamiento diferenciado en función de los sustratos, apreciable en todas las variables estudiadas (nutricionales, fúngicas y fisiológicas, lo cual demostró la factibilidad del uso de dichos microorganismos en este

  13. Cardiovascular effects of hemoglobin response in patients receiving epoetin alfa and oral iron in heart failure with a preserved ejection fraction

    Institute of Scientific and Technical Information of China (English)

    Sirish Vullaganti; Jeff Goldsmith; Sergio Teruya; Julissa Alvarez; Stephen Helmke; Mathew S.Maurer

    2014-01-01

    Background Previous data from a recently conducted prospective, single blind randomized clinical trial among community dwelling older patients with heart failure with a preserved ejection fraction (HFPEF) and anemia randomized to treatment with epoetin alfa (erythro-poiesis-stimulating agents, ESA) vs. placebo did not demonstrate significant benefits of therapy regarding left ventricular (LV) structure, functional capacity, or quality of life (QOL). However, several patients randomized to the treatment arm were non-responders with a subop-timal increase in hemoglobin. All patients in the trial also received oral ferrous gluconate, which could have contributed to increases in he-moglobin observed in those receiving placebo. Accordingly, we performed an analysis separating patients into responders vs. non-responders in order to determine if measured improvement in anemia would have any effect on clinical endpoints. Methods A total of 56 patients (age 77 ± 11 years, 68%female) were recruited who had anemia defined as a hemoglobin of≤12 g/dL (average, 10.4 ± 1 g/dL) with HFPEF defined as having NHANES-CHF (National Health And Nutrition Examination Survey:Congestive Heart Failure) criteria score of≥3 and an ejection fraction of>40%(average EF=63%±15%). Patients were randomly allocated to receive either ESA and ferrous gluconate or ferrous gluconate only. In this analysis, a responder was defined as a patient with an increase of 1 g/dL in the first 4 weeks of the trial. Re-sults Nineteen subjects were classified as responders compared to 33 non-responders. While the average hemoglobin increased signifi-cantly at the end of 6 months for responders (1.8 ± 0.3 vs. 0.8 ± 0.2 g/dL, P = 0.004), 50% of the subjects assigned to ESA were non-responders. Left ventricular function including ejection fraction (P=0.32) and end diastolic volume (P=0.59) was unchanged in res-ponders compared to non-responders. Responders also showed no significant improvements in New York

  14. HI Absorption Lines Detected from the Arecibo Legacy Fast ALFA Survey Data

    Science.gov (United States)

    Zhong-zu, Wu; Martha P, Haynes; Riccardo, Giovanelli; Ming, Zhu; Ru-rong, Chen

    2015-10-01

    We present some preliminary results of an on-going study of HI 21-cm absorption lines based on the 40% survey data released by the Arecibo Legacy Fast Arecibo L-band Feed Array (ALFALFA). (1) Ten HI candidate absorbers have been detected. Five of them are previously published in the literature, and the rest of them are new detections that need further confirmation. (2) For those sources with no detected absorptions, we have calculated the upper limit of their foreground HI column density NHI. The statistical result of the NHI distribution indicates that the ratio Ts/f between the averaged spin temperature and coverage factor for DLAs (the damped Lyα systems) might be larger than 500 K. The radio frequency interference (RFI) and standing wave are the main factors affecting the detection of HI absorption lines, which have been analyzed and discussed as well in order to find a method of solution. Our study can serve as a pathfinder for the future large-scale search of HI 21-cm absorption lines using the Five-Hundred-Meter Aperture Spherical Radio Telescope (FAST), which is an Arecibo-type radio telescope currently under construction in China with greatly increased sensitivity, bandwidth, and observational sky area. As prospects, we have discussed two types of observational studies of HI absorption lines toward extragalactic sources using the FAST telescope.

  15. The Arecibo Legacy Fast ALFA Survey: The Galaxy Population Detected by ALFALFA

    CERN Document Server

    Huang, Shan; Giovanelli, Riccardo; Brinchmann, Jarle

    2012-01-01

    Making use of HI 21 cm line measurements from the ALFALFA survey (alpha.40) and photometry from the Sloan Digital Sky Survey (SDSS) and GALEX, we investigate the global scaling relations and fundamental planes linking stars and gas for a sample of 9417 common galaxies: the alpha.40-SDSS-GALEX sample. In addition to their HI properties derived from the ALFALFA dataset, stellar masses (M_*) and star formation rates (SFRs) are derived from fitting the UV-optical spectral energy distributions. 96% of the alpha.40-SDSS-GALEX galaxies belong to the blue cloud, with the average gas fraction f_HI = M_HI/M_* ~ 1.5. A transition in SF properties is found whereby below M_* ~ 10^9.5 M_sun, the slope of the star forming sequence changes, the dispersion in the specific star formation rate (SSFR) distribution increases and the star formation efficiency (SFE) mildly increases with M_*. The evolutionary track in the SSFR-M_* diagram, as well as that in the color magnitude diagram are linked to the HI content; below this trans...

  16. LOCAL APPLICATION OF RECOMBINANT INTERFERON-ALFA2 FOR TREATMENT OF RECURRENT RESPIRATORY PAPILLOMATOSIS

    Directory of Open Access Journals (Sweden)

    M. Plouzhnikov

    2006-01-01

    Full Text Available Abstract. Recurrent respiratory papillomatosis (RRP is the most frequently occurring tumour of the upper airways associated with a human papilloma virus (HPV. The aim of this study was to reveal some features of systemic and local immunity in RRP, to investigate clinical and immunological efficiency of local treatment with recombinant interferon-α (rIFNα, and to determine clinical and laboratory indications to it’s administration. The study included forty-one patients with confirmed RRP. Their examination included histological examination of papillomas, detection of HPV DNA in papilloma tissues using PCR technique, phenotyping of circulating lymphocytes (CD3+, CD4+, CD8+, CD25+, HLA-DR+ by means of flow cytometry. The levels of IFNγ, TNFα, GM-CSF, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13 in laryngeal secretions were quantified by a multiplex immunoassay. In all cases, we revealed an initially decreased functional activity of Т-lymphocytes, as well as low contents of Т-killer and NK-cells. In laryngeal secretions, increased values of Th1-type-specific cytokines (IFNγ and TNFα were found. Besides that, high levels of local IL-4 were detected thus being typical to alternative Th2-type response. Single inhalations 1000 000 ME of «Interal» or «Roferon» preparations were administered daily (a total of 10-15 millions ME per therapeutic course. Thirteen patients received the treatment after surgery, as an adjuvant therapy, and eleven patients underwent monotherapy. Complete tumor regression of tumors following this monotherapy was observed in 45,5% of the patients, whereas partial regression was registered in 45%. The effect was mostly expressed in frequently recurring juvenile papillomatosis with aggressive course and histological pattern of actively proliferating papilloma. In the patients with complete tumor regression, high initial levels of TNFα and IL-4/IFNг ratios were revealed initially in laryngeal secretions. When rIFNб was

  17. Neutron-induced alpha radiography; Radiografia com particulas alfa induzida por neutrons

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Marco Antonio Stanojev

    2008-07-01

    A new radiography technique to inspect thin samples was developed. Low energy alpha particles, generated by a boron based screen under thermal neutron irradiation, are used as penetrating radiation. The solid state nuclear track detector CR-39 has been used to register the image. The interaction of the {alpha} - particles with the CR-39 gives rise to damages which under an adequate chemical etching became tracks the basic units forming the image. A digital system was developed for data acquisition and data analysis as well as for image processing. The irradiation and etching conditions to obtain the best radiography are 1,3 hours and 25 minutes at 70 deg C respectively. For such conditions samples having 10 {mu}m in thickness can be inspected with a spatial resolution of 32 {mu}m. The use of the digital system has reduced the time spent for data acquisition and data analysis and has improved the radiography image visualization. Furthermore, by using the digital system, it was possible to study several new parameters regarding the tracks which are very important to understand and study the image formation theory in solid state nuclear track detectors, the one used in this thesis. Some radiography images are also shown which demonstrate the potential of the proposed radiography technique. When compared with the other radiography techniques already in use to inspect thin samples, the present one developed in the present paper allows a smaller time to obtain the image, it is not necessary to handle liquid radioactive substances, the detector is insensitive to {beta}, {gamma}, X-ray and visible light. (author)

  18. Associação de bronquite plástica com enteropatia perdedora de proteínas após operação de Fontan Asociación de bronquitis plástica a la enteropatía perdedora de proteínas tras operación de fontan Association of plastic bronchitis to protein-losing enteropathy after fontan operation

    Directory of Open Access Journals (Sweden)

    Vanessa Alves Guimarães

    2010-04-01

    Full Text Available Relatamos um caso incomum de associação de bronquite plástica (BP com enteropatia perdedora de proteínas (EPP em menina de 4 anos e 9 meses de idade. com dupla via de entrada de ventrículo único tipo esquerdo e concordância ventrículo-arterial submetida à cirurgia cavopulmonar total. com túnel lateral intracardíaco aos três anos. Tornaram-se chamativas a eliminação de molde brônquico de fibrina de 10 cm (BP e a elevação de alfa-1-antitripsina de 52 mg/g de fezes. Em uso de sildenafila. programou-se. em caso de continuidade do processo. a ligadura do ducto torácico e transplante cardíaco.Referimos el caso atípico de asociación de bronquitis plástica (BP con enteropatía perdedora de proteínas (EPP en una menina de 4 años y 9 meses de edad, con doble vía de entrada de ventrículo único tipo izquierdo y concordancia ventrículo-arterial sometida a la cirugía cavopulmonar total, con túnel lateral intracardiaco a los 3 años. Se consideraron como llamativas la eliminación de molde bronquial de 10 cm formado por fibrina (BP y la elevación de alfa-1-antitripsina de 52 mg/g de heces. En uso de sildenafila, se programó, en caso de continuidad del proceso, la ligadura del conducto torácico y trasplante cardíaco.We report an unusual case of association of plastic bronchitis (PB to protein-losing enteropathy (PLE in a girl of 4 years and 9 months of age with double inlet single left ventricle and ventriculoarterial concordance. submitted to total cavopulmonary surgery. with an intracardiac lateral tunnel at the age of three. The elimination of the 10 cm fibrin bronchial mold (PB and the alpha-1-antitrypsin elevation of 52 mg/g in feces had both become outstanding. Using sildenafil. the thoracic duct ligature and the cardiac transplant were programmed in case of continuity of the process.

  19. Randomized comparison of low-dose versus high-dose interferon-alfa in chronic myeloid leukemia: prospective collaboration of 3 joint trials by the MRC and HOVON groups.

    NARCIS (Netherlands)

    Kluin-Nelemans, H.C.; Buck, G.; Cessie, S. le; Richards, S.; Beverloo, H.B.; Falkenburg, J.H.F.; Littlewood, T.; Muus, P.; Bareford, D.; Lelie, H. van der; Green, A.R.; Roozendaal, K.J.; Milne, A.E.; Chapman, C.S.; Shepherd, P.

    2004-01-01

    The optimal dose of interferon-alfa (IFN) for chronic myeloid leukemia (CML) is unknown. Retrospective analyses suggest that low doses are as effective as high doses, with less toxicity and fewer patients abandoning the drug. The Dutch Hemato-Oncology Association (HOVON) and British Medical Research

  20. Prediction of Ovarian Hyperstimulation Syndrome in Patients Treated with Corifollitropin alfa or rFSH in a GnRH Antagonist Protocol.

    Directory of Open Access Journals (Sweden)

    Georg Griesinger

    Full Text Available What is the threshold for the prediction of moderate to severe or severe ovarian hyperstimulation syndrome (OHSS based on the number of growing follicles ≥ 11 mm and/or estradiol (E2 levels?The optimal threshold of follicles ≥11 mm on the day of hCG to identify those at risk was 19 for both moderate to severe OHSS and for severe OHSS. Estradiol (E2 levels were less prognostic of OHSS than the number of follicles ≥ 11 mm.In comparison to long gonadotropin-releasing hormone (GnRH agonist protocols, the risk of severe OHSS is reduced by approximately 50% in a GnRH antagonist protocol for ovarian stimulation prior to in vitro fertilisation (IVF, while the two protocols provide equal chances of pregnancy per initiated cycle. Nevertheless, moderate to severe OHSS may still occur in GnRH antagonist protocols if human chorionic gonadotropin (hCG is administered to trigger final oocyte maturation, especially in high responder patients. Severe OHSS following hCG trigger may occur with an incidence of 1-2% in a relatively young (aged 18 to 36 years IVF population treated in a GnRH-antagonist protocol.From the Engage, Ensure and Trust trials, in total, 2,433 women who received hCG for oocyte maturation and for whom the number of follicles ≥ 11 mm and the level of E2 on the day of hCG administration were known were included in the analyses.The threshold for OHSS prediction of moderate and severe OHSS was assessed in women treated with corifollitropin alfa or daily recombinant follicle stimulation hormone (rFSH in a gonadotropin-releasing hormone (GnRH-antagonist protocol. Receiver operating characteristics curve analyses for moderate to severe OHSS and severe OHSS were performed on the combined dataset and the sensitivity and specificity for the optimal threshold of number of follicles ≥ 11 mm, E2 levels on the day of (hCG, and a combination of both, were determined.The optimal threshold of follicles ≥ 11 mm on the day of hCG to identify those at

  1. Prediction of Ovarian Hyperstimulation Syndrome in Patients Treated with Corifollitropin alfa or rFSH in a GnRH Antagonist Protocol

    Science.gov (United States)

    Griesinger, Georg; Verweij, Pierre J. M.; Gates, Davis; Devroey, Paul; Gordon, Keith; Stegmann, Barbara J.; Tarlatzis, Basil C.

    2016-01-01

    Study Question What is the threshold for the prediction of moderate to severe or severe ovarian hyperstimulation syndrome (OHSS) based on the number of growing follicles ≥ 11 mm and/or estradiol (E2) levels? Summary Answer The optimal threshold of follicles ≥11 mm on the day of hCG to identify those at risk was 19 for both moderate to severe OHSS and for severe OHSS. Estradiol (E2) levels were less prognostic of OHSS than the number of follicles ≥ 11 mm. What Is Known Already In comparison to long gonadotropin-releasing hormone (GnRH) agonist protocols, the risk of severe OHSS is reduced by approximately 50% in a GnRH antagonist protocol for ovarian stimulation prior to in vitro fertilisation (IVF), while the two protocols provide equal chances of pregnancy per initiated cycle. Nevertheless, moderate to severe OHSS may still occur in GnRH antagonist protocols if human chorionic gonadotropin (hCG) is administered to trigger final oocyte maturation, especially in high responder patients. Severe OHSS following hCG trigger may occur with an incidence of 1–2% in a relatively young (aged 18 to 36 years) IVF population treated in a GnRH-antagonist protocol. Study Design, Size, Duration From the Engage, Ensure and Trust trials, in total, 2,433 women who received hCG for oocyte maturation and for whom the number of follicles ≥ 11 mm and the level of E2 on the day of hCG administration were known were included in the analyses. Participants/Materials, Setting, Methods The threshold for OHSS prediction of moderate and severe OHSS was assessed in women treated with corifollitropin alfa or daily recombinant follicle stimulation hormone (rFSH) in a gonadotropin-releasing hormone (GnRH)-antagonist protocol. Receiver operating characteristics curve analyses for moderate to severe OHSS and severe OHSS were performed on the combined dataset and the sensitivity and specificity for the optimal threshold of number of follicles ≥ 11 mm, E2 levels on the day of (hCG), and a

  2. Darbepoetin Alfa Injection

    Science.gov (United States)

    ... check that the needle is covered with the grey cover and that the yellow plastic sleeve has ... the FDA's Safe Disposal of Medicines website (http://goo.gl/c4Rm4p) for more information if you do ...

  3. Effect of peginterferon alfa-2a in treating lamivudine-resistant HBeAg-positive chronic hepatitis B%聚乙二醇化干扰素α-2a对拉米夫定治疗失败的HBeAg阳性慢性乙型肝炎患者的疗效

    Institute of Scientific and Technical Information of China (English)

    马晓宇; 石小枫; 郭树华; 刘杞; 任红

    2013-01-01

    目的 探讨拉米夫定治疗失败的HBeAg阳性慢性乙型肝炎患者,接受聚乙二醇化干扰素α-2a(PEGIFNα-2a)序贯治疗的临床疗效.方法 对22例经拉米夫定治疗失败的HBeAg阳性慢性乙型肝炎患者,在继续使用拉米夫定的情况下,加用PEGIFNα-2a 180 μg,每周1次,联用12周,然后停用拉米夫定,单用PEGIFNα-2a至48周,每月检查1次肝功能、血常规;每3个月检查乙型肝炎病毒(HBV)血清标志物及HBV DNA定量;治疗结束后随访48周.结果 HBV DNA基线平均表达水平为(6.54±0.90)copies/mL(log10).在治疗结束后,有40.91%(9/22)的患者实现HBeAg血清转换和HBV DNA血清表达水平减少,13.64%(3/22)的患者实现HBsAg血清转换.随访48周,36.36%(8/22)的患者维持HBeAg血清转换和HBV DNA水平的降低,在维持HBeAg血清学转换的8例患者中,有2例患者持续维持HBsAg血清转换.结论 PEGIFNα-2a能部分明显降低拉米夫定治疗失败患者的HBV DNA水平,促进HBe Ag和HBs Ag的血清学转换.%Objective To observe the clinical effect of pegylated interferon alfa-2a sequential therapy in the patients with lamivudine-resistant HBeAg-positive chronic hepatitis B (CHB) .Methods A total of 22 patients with lamivudine-resistant HBeAg-posi-tive chronic hepatitis were included in the study .Under the continuous use of lamivudine(100 mg ,once daily) ,all patients received peginterferon alfa-2a 180 g ,once weekly for 12 weeks .Then lamivudine was discontinued and peginterferon alfa-2a as monotherapy was used till 48 weeks .Responses were measured once every 12 weeks after treatment ,including HBsAg , HBeAg seroconversion and HBV DNA suppression .The liver function and blood routine test were examined once every 4 weeks .Results Mean HBV DNA level at baseline was (6 .54±0 .90)copies/mL(log10) .At the end of treatment ,40 .91% (9/22) of the patients had HBeAg seroconversion and the decreased serum expression level of HBV DNA ,and 13 .64% (3/22) of the patients

  4. Cryoglobulinemia in chronic hepatitis C: clinical aspects and response to treatment with interferon alpha and ribavirin Crioglobulinemia na hepatite crônica C: aspectos clínicos e resposta ao tratamento com interferon alfa e ribavirina

    Directory of Open Access Journals (Sweden)

    Edison Roberto Parise

    2007-04-01

    Full Text Available INTRODUCTION: The main extra-hepatic manifestation of hepatitis C is mixed cryoglobulinemia (MC. The aim of this study was to evaluate its prevalence among patients with chronic hepatitis C (CHC, to correlate its presence to host and virological variables and to the response to combined therapy with interferon-alpha and ribavirin. CASUISTIC AND METHODS: 202 CHC naive patients (136 with chronic hepatitis and 66 with cirrhosis were consecutively evaluated for the presence of cryoglobulins. Cryoprecipitates were characterized by immunoelectrophoresis and classified according to the Brouet's criteria. RESULTS: The prevalence of MC was 27% (54/202, and 24% of them (13/54 showed major clinical manifestation of the disease. Even though type III MC was more frequent (78%, symptomatic MC was more common in type II MC. The presence of cirrhosis (RR = 2.073; IC95% = 1.029 - 4.179; p = 0.041, and age of the patients (RR = 1.035; IC95% = 1.008 - 1.062; p = 0.01 were independently associated with the presence of cryoglobulins. No relationship was found with viral load and genotype. 102 patients were treated with interferon alpha and ribavirin. Among these, 31 had MC. Sustained virological response (around 30% was similar in patients with and without MC (p = 0.971. CONCLUSION: MC represents a prevalent complication in patients with CHC, specially older and cirrhotic patients. Only 24% of these patients show clinical manifestation of the disease, specially those with type II MC. The presence of MC did not affect the response to therapy.INTRODUÇÃO: A crioglobulinemia mista (CM representa importante complicação extra-hepática da hepatite C. Nesse estudo avaliamos sua prevelência em pacientes com hepatite C crônica (HCC e relacionamos sua presença com aspectos clínicos e resposta ao tratamento com interferon alfa e ribavirina. CASUÍSTICA E MÉTODOS: 202 pacientes consecutivos com HCC (136 com hepatite crônica e 66 com cirrose foram avaliados para a

  5. Bronquite plástica em criança com talassemia alfa Plastic bronchitis in a child with thalassemia alpha

    OpenAIRE

    Tiago N. Veras; Gustavo M. Lannes; Jefferson P. Piva; Renato T. Stein; Marcus H. Jones; Vinicius D. Silva; Pedro Celiny R Garcia; Pitrez, Paulo M. C.

    2005-01-01

    OBJETIVO: A bronquite plástica é uma doença infreqüente na criança, sendo caracterizada por moldes ou cilindros mucofibrinosos na árvore traqueobrônquica. Faz parte do diagnóstico diferencial de crianças com insuficiência respiratória de início agudo, e o tratamento precoce é importante para a resolução do quadro. O objetivo deste relato é descrever um caso de bronquite plástica tratado com sucesso por endoscopia, em paciente portador de talassemia alfa. DESCRIÇÃO: Criança do sexo masculino, ...

  6. Análisis de consistencia interna mediante Alfa de Cronbach: un programa basado en gráficos dinámicos

    OpenAIRE

    Rubén Ledesma; Gabriel Molina Ibañez; Pedro Valero Mora

    2002-01-01

    En este trabajo se presenta una herramienta informática original que permite realizar análisis de consistencia interna (modelo Alfa de Cronbach) utilizando métodos gráficos dinámicos. Se trata de un módulo basado en la filosofía del Análisis Exploratorio de Datos y en métodos de visualización estadística, diseñado para asistir al analista en el proceso de construcción de pruebas psicológicas. La herramienta permite analizar la consistencia interna de la prueba, las propiedades de los ítems qu...

  7. Multivariate regression analyses of data from a randomised, double-blind, placebo-controlled study confirm quality of life benefit of epoetin alfa in patients receiving non-platinum chemotherapy

    OpenAIRE

    Fallowfield, L; Gagnon, D.; Zagari, M; Cella, D.; Bresnahan, B; Littlewood, T J; McNulty, P.; Gorzegno, G; Freund, M.

    2002-01-01

    Cancer-related anaemia is associated with a wide spectrum of symptoms that can negatively affect quality of life. Because epoetin alfa has demonstrated efficacy in correcting cancer-related anaemia, the impact of this treatment on quality of life was evaluated in a multinational, randomised, double-blind, placebo-controlled trial in 375 anaemic cancer patients receiving non-platinum-based chemotherapy. The cancer-specific measures of quality of life included the general scale (FACT-G Total) a...

  8. Bronquite plástica em criança com talassemia alfa Plastic bronchitis in a child with thalassemia alpha

    Directory of Open Access Journals (Sweden)

    Tiago N. Veras

    2005-12-01

    Full Text Available OBJETIVO: A bronquite plástica é uma doença infreqüente na criança, sendo caracterizada por moldes ou cilindros mucofibrinosos na árvore traqueobrônquica. Faz parte do diagnóstico diferencial de crianças com insuficiência respiratória de início agudo, e o tratamento precoce é importante para a resolução do quadro. O objetivo deste relato é descrever um caso de bronquite plástica tratado com sucesso por endoscopia, em paciente portador de talassemia alfa. DESCRIÇÃO: Criança do sexo masculino, 3 anos de idade, sem antecedentes mórbidos significativos, apresentou quadro de insuficiência respiratória aguda, com achados radiológicos de atelectasia pulmonar sugestivos de aspiração de corpo estranho. Não havia sintomas respiratórios ou antecedentes de alergia ou infecções respiratórias de repetição. A realização de broncoscopia flexível, complementada por endoscopia rígida e exame anatomopatológico, evidenciou a presença de bronquite plástica. Após a realização da endoscopia, a criança evoluiu satisfatoriamente, com curva térmica afebril e extubação em 72 horas. Foram utilizadas medicações sintomáticas, sem necessidade de antimicrobianos. Dez dias após a alta, a radiografia de tórax encontrava-se normal. A talassemia alfa foi diagnosticada através da eletroforese de hemoglobina. COMENTÁRIOS: A importância clínica da bronquite plástica reside no fato de apresentar um quadro semelhante ao de outras doenças mais prevalentes, como a aspiração de corpo estranho e a asma brônquica. A suspeita do quadro recomenda a realização de endoscopia para diagnóstico e tratamento. É reconhecida a ocorrência de bronquite plástica em crianças com fibrose cística, pós-operatório de cirurgia cardíaca e anemia falciforme. No presente artigo, foi observada uma associação com talassemia alfa.OBJECTIVE: Plastic bronchitis is an unusual condition in children, associated with formation of mucofibrinous

  9. Deficiência de 5alfa-redutase tipo 2: experiências de Campinas (SP e Salvador (BA 5alpha-reductase type 2 deficiency: experiences from Campinas (SP and Salvador (BA

    Directory of Open Access Journals (Sweden)

    Christine Hackel

    2005-02-01

    Full Text Available OBJETIVO: Apresentar a experiência relativa a pacientes com deficiência da enzima 5alfa-redutase tipo 2 provenientes de três serviços distintos no Brasil. CASUÍSTICA E MÉTODOS: Foram incluídos 25 pacientes com sinais clínicos e hormonais de deficiência da 5alfa-redutase 2 pertencentes a 23 famílias, 15 oriundas da Bahia, 7 de São Paulo e 1 de Minas Gerais. Foram avaliados dados clínicos, hormonais e moleculares. A análise molecular dos 5 éxons do gene SRD5A2 foi feita por meio da técnica de PCR, seguida de seqüenciamento automático ou manual. RESULTADOS: Em 10 famílias havia mutações no gene SRD5A2 em homozigose (5 com G183S, 2 com R246W, 1 com G196S, 1 com del642T, 1 com 217_218insC e em 3 em heterozigose composta (1 com Q126R/IVS3+1G>A, 1 com Q126R/del418T e 1 com Q126R/G158R; em 3 casos os afetados eram heterozigotos, apresentando apenas uma mutação deletéria (1 com G196S, 1 com A207D e 1 com R246W. Em 7 casos não foram detectadas anormalidades ao seqüenciamento. Observou-se maior freqüência da G183S em pacientes miscigenados (Afro-Euro-Brasileiros oriundos da Bahia. Os achados clínicos e hormonais não diferiram entre os casos com e sem mutação, à exceção da freqüência de consangüinidade e da maior gravidade da ambigüidade genital nos primeiros. CONCLUSÕES: Os resultados encontrados salientam a importância da investigação molecular para o diagnóstico dessa doença, ressaltando o achado de uma mutação bastante freqüente em nosso meio (G183S, especialmente em pacientes miscigenados oriundos da Bahia, e a descrição de mutações que até o momento só foram relatadas em pacientes brasileiros.OBJECTIVE: To report the experience regarding patients with steroid 5alpha-reductase type 2 deficiency from three different clinical services in Brazil. CASUISTIC AND METHODS: Twenty five patients with clinical and hormonal features of 5alpha-reductase deficiency from 23 families (15 from Bahia, 7 from S

  10. Composicao quimica, perfil de acidos graxos e quantificacao dos acidos ƒ¿-linolenico, eicosapentaenoico e docosahexaenoico em visceras de tilapias (Oreochromis niloticus = Percentual composition, fatty acids and quantification of the LNA (Alfa-Linolenic, EPA (Eicosapentaenoic and DHA (Docosahexaenoic acids in visceras of Nile Tilapia (Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Nilson Evelázio de Souza

    2005-01-01

    Full Text Available Foi avaliada a composição química de vísceras de tilápias (Oreochromis niloticus criadas em cativeiro Os teores de umidade, cinza, proteína bruta e lipídios totais foram de 64,4%; 1,3%; 6,3% e 18,0%, respectivamente, caracterizando alta concentração de lipídiostotais em relação a outros resíduos de peixes. Foram identificados 49 ácidos graxos, sendo majoritários os ácidos: oléico, (32,8%, seguido do palmítico, (19,9% e linoléico, (18,2%. As razões entre n-6/n-3 e ácidos poliinsaturados/saturados foram de 5,5 e 0,9, respectivamente. As quantificações dos ácidos graxos alfa-linolênico, eicosapentaenóico e docosahexaenóico, em mg/g de lipídios totais, foram de 10,4, 1,4 e 9,3, respectivamente. O elevado teor de lipídios totais das vísceras contribuiu significativamente para as quantidadesde ácidos graxos n-3. Todos os parâmetros analisados foram satisfatórios sob o ponto de vista nutricional e neste sentido as vísceras de tilápias poderão ser utilizadaa para alimentar peixes ou outros animais.The chemical composition was evaluated in visceras of tilapias raised in captivity. The moisture, ash, crude protein and total lipids contents were 64.4%; 1.3%; 6.3% and 18.0%, respectively, characterizing high total lipids concentration in relation other residues of fish. Forty nine fatty acids were detected, the major fatty acids were oleic (32.8%, palmitic (19.9% and linoleic-1 (18.2% and oleic (9.4%. The ratio n-6/n-3 and polyunsaturated/saturated fatty acids, showed the values 5.5 and 0.9, respectively. The quantifications of alfa-linolenic, eicosapentaenoic and docosahexaenoic acids (in mg/g of total lipids, were 10.4, 1.4 and 0.3, respectively. The higher contents of total lipids in visceras contributed significantly for amounts of n-3 fatty acids. All the parameters analyzed were shown nutritional value satisfactory in this sense visceras of tilapias can be used in the feed of fish and other animal.

  11. Estratégia de troca entre agentes anti-TNF-alfa não melhora a capacidade funcional em pacientes com artrite reumatoide de longa evolução Switching between anti-TNF-alpha agents does not improve functional capacity in patients with long-standing and active rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    Maria Roberta Melo P Soares

    2012-02-01

    Full Text Available OBJETIVOS: Avaliar a resposta clínica após a estratégia de troca entre agentes antifator de necrose tumoral alfa (anti-TNF-alfa em pacientes com artrite reumatoide (AR. PACIENTES E MÉTODOS: Foram incluídos 99 pacientes com diagnóstico de AR (American College of Rheumatology, 1987, em uso de terapia anti-TNF-alfa, para avaliação da resposta terapêutica após 24 semanas. A estratégia de troca foi feita se, após 12 a 24 semanas, houvesse relato de evento adverso sério (T: toxicidade ou se não ocorresse redução maior que 0,6 do índice de atividade da doença (DAS28 inicial (RI: resposta inadequada. Nesse último caso, o paciente foi considerado como falência primária (FP. Falência secundária (FS foi definida se houvesse perda de resposta após melhora inicial. Remissão (DAS28 0,2 do questionário de avaliação da saúde (HAQ inicial] foram avaliadas por análise de regressão linear. P OBJECTIVES: To assess clinical response after switching between anti-tumor necrosis factor-alpha (anti-TNF-alpha agents in patients with rheumatoid arthritis (RA. PATIENTS AND METHODS: This study included 99 patients diagnosed with RA American College of Rheumatology, 1987, on anti-TNF-alpha therapy, to assess the therapeutic response after 24 weeks. Switching was performed if, after 12 to 24 weeks, a severe adverse event was reported (toxicity: T or if no reduction greater than 0.6 in the initial Disease Activity Score 28 (DAS28 occurred (inadequate response: IR. In case of IR, the patient was considered as primary failure (PF. Secondary failure (SF was defined as loss of response after initial improvement. Remission (DAS28 0.2] were assessed by use of linear regression analysis. The significance level adopted was P < 0.05. RESULTS: Switching was performed in 39 (39.4% patients, especially due to PF (24.3%, SF (35.1% and T (40.5%. The retention rate of the first agent was 60.1%, and the mean time for switching was 14.2 ± 10.9 months. After

  12. Herbal compound 'Songyou Yin' reinforced the ability of interferon-alfa to inhibit the enhanced metastatic potential induced by palliative resection of hepatocellular carcinoma in nude mice

    International Nuclear Information System (INIS)

    Liver resection is a widely accepted treatment for hepatocellular carcinoma (HCC). Our previous clinical study showed that the rate of palliative resection was 34.0% (1958-2008, 2754 of 8107). However, the influence of palliative resection on tumor metastasis remains controversial. The present study was conducted to evaluate the effect of palliative resection on residual HCC and to explore interventional approaches. Palliative resection was done in an orthotopic nude mice model of HCC (MHCC97H) with high metastatic potential. Tumor growth, invasion, metastasis, lifespan, and some molecular alterations were examined in vivo and in vitro. Mice that underwent palliative resection were treated with the Chinese herbal compound 'Songyou Yin,' interferon-alfa-1b (IFN-α), or their combination to assess their effects. In the palliative resection group, the number of lung metastatic nodules increased markedly as compared to the sham operation group (14.3 ± 4.7 versus 8.7 ± 3.6, P < 0.05); tumor matrix metalloproteinase 2 (MMP2) activity was elevated by 1.4-fold, with up-regulation of vascular endothelial growth factor (VEGF) and down-regulation of tissue inhibitor of metalloproteinase 2 (TIMP2). The sera of mice undergoing palliative resection significantly enhanced cell invasiveness by 1.3-fold. After treatment, tumor volume was 1205.2 ± 581.3 mm3, 724.9 ± 337.6 mm3, 507.6 ± 367.0 mm3, and 245.3 ± 181.2 mm3 in the control, 'Songyou Yin,' IFN-α, and combination groups, respectively. The combined therapy noticeably decreased the MMP2/TIMP2 ratio and prolonged the lifespan by 42.2%. Moreover, a significant (P < 0.001) reduction of microvessel density was found: 43.6 ± 8.5, 34.5 ± 5.9, 23.5 ± 5.6, and 18.2 ± 8.0 in the control and treatment groups, respectively. Palliative resection-stimulated HCC metastasis may occur, in part, by up-regulation of VEGF and MMP2/TIMP2. 'Songyou Yin' reinforced the ability of IFN-α to inhibit the

  13. Discovery and basic pharmacology of erythropoiesis-stimulating agents (ESAs), including the hyperglycosylated ESA, darbepoetin alfa: an update of the rationale and clinical impact.

    Science.gov (United States)

    Kiss, Zoltán; Elliott, Steven; Jedynasty, Kinga; Tesar, Vladimír; Szegedi, János

    2010-04-01

    Cloning of the human erythropoietin (EPO) gene and development of the first recombinant human erythropoietin (rHuEPO) drug were truly breakthroughs. This allowed a deeper understanding of the structure and pharmacology of rHuEpo, which in turn inspired the discovery and development of additional erythropoiesis-stimulating agents (ESAs). In vivo specific activity and serum half-life of rHuEPO are influenced by the amount and structure of the attached carbohydrate. Increased numbers of sialic acids on carbohydrate attached to rHuEPO correlated with a relative increase in in-vivo-specific activity and increased serum half-life. The effect of increasing the number of sialic-acid-containing carbohydrates on in-vivo-specific activity was explored. Initial research focused on solving the problem of how the protein backbone could be engineered so a cell would add more carbohydrate to it. Additional work resulted in darbepoetin alfa, a longer-acting molecule with two additional carbohydrate chains. PMID:20127232

  14. THE ARECIBO LEGACY FAST ALFA SURVEY. V. THE H I SOURCE CATALOG OF THE ANTI-VIRGO REGION AT δ = +270

    International Nuclear Information System (INIS)

    We present a second catalog of H I sources detected in the Arecibo Legacy Fast ALFA Survey. We report 488 detections over 135 deg2, within the region of the sky having 22 h 0 0. We present here the detections that have either (a) S/N>6.5, where the reliability of the catalog is better than 95% or (b) 5.0 Hi7.5 Msun are satellites of either the NGC672/IC1727 nearby galaxy pair or their neighboring dwarf irregular galaxy NGC784. The data of this catalog release include a slice through the Pisces-Perseus foreground void, a large nearby underdensity of galaxies. We report no detections within the void, where our catalog is complete for systems with H i masses of 108 Msun. Gas-rich, optically-dark galaxies do not seem to constitute an important void population, and therefore do not suffice for producing a viable solution to the void phenomenon.

  15. CXCL9 associated with sustained virological response in chronic hepatitis B patients receiving peginterferon alfa-2a therapy: a pilot study.

    Directory of Open Access Journals (Sweden)

    I-Cheng Lee

    Full Text Available BACKGROUND AND AIMS: There is lack of a practical biomarker to predict sustained virological response (SVR in chronic hepatitis B (CHB patients undergoing peginterferon alfa-2a (PEG-IFN. The aim of this pilot study was to identify immunological features associated with SVR. METHODS: Consecutive 74 CHB patients receiving 24 weeks (for hepatitis B e antigen (HBeAg-positive or 48 weeks (for HBeAg-negative PEG-IFN, were prospectively enrolled. Serum HBV viral loads, hepatitis B surface antigen (HBsAg, CXCL9, IFN-γ-inducible protein 10 (IP-10, interferon-gamma (IFN-γ and transforming growth factor beta (TGF-β were measured at baseline and week 12. SVR was defined as HBeAg seroconversion combined with viral load 80 pg/mL, HBV DNA 10% at week 12 were predictors of SVR. The performance of CXCL9 in predicting SVR was good in patients with HBV DNA <2.5 x 10(7 IU/mL, particularly in HBeAg-negative CHB cases (positive predictive value, PPV= 64.3%. CONCLUSIONS: Pre-treatment CXCL9 level has the potential to select CHB patients who can respond to PEG-IFN, especially in HBeAg-negative patients with low viral loads.

  16. Long term response to interferon treatment in chronic hepatitis C patients is associated with a significant reduction in anti-E1 envelope antibody titers

    NARCIS (Netherlands)

    Depraetere, S; Van Kerschaever, E; Van Vlierberghe, H; Elewaut, A; Brouwer, J T; Niesters, H G; Schalm, S W; Maertens, G; Leroux-Roels, G

    2000-01-01

    Interferon (IFN) alfa has been used widely for the treatment of chronic hepatitis C virus (HCV) infections but only a small number of patients treated have shown a sustained biochemical and virological response. Anti-envelope E1 and E2 antibody titers were assessed retrospectively before, during, an

  17. Níveis de alfa-tocoferol no soro e leite materno de puérperas atendidas em maternidade pública de Natal, Rio Grande do Norte Levels of alpha-tocopherol in the serum and breast-milk of child-bearing women attending a public maternity hospital in the city of Natal , in the Brazilian State of Rio Grande do Norte

    Directory of Open Access Journals (Sweden)

    Lígia Rejane Siqueira Garcia

    2009-12-01

    Full Text Available OBJETIVOS: avaliar os níveis de alfa-tocoferol no soro e leite materno em diferentes estágios de lactação de puérperas e verificar a adequação nutri cional de vitamina E do leite oferecido ao lactente. MÉTODOS: participaram do estudo 32 parturientes adultas com idade média de 25 anos. Foram coletados 5 mL de sangue e 2 mL de colostro, em condição de jejum, para análise dos níveis de alfa tocoferol. Entre 10 e 15 dias pós-parto foram coletados mais 2 mL de leite. As amostras foram analisadas por Cromatografia Líquida de Alta Eficiência. A adequação nutricional do leite para a vitamina E foi calculada pelo produto do volume estimado de ingestão de leite com a concentração de α-tocoferol no leite e por comparação direta desse produto com o valor de referência para ingestão do nutriente (4 mg/dia. RESULTADOS: os níveis de alfa-tocoferol no sangue foram 29 ± 0,9 µmol/L (Média ± Erro padrão e no colostro e leite de transição foram 28,7 ± 4,7 µmol/L e 7,8 ± 1,0 µmol/L, respectivamente. O consumo estimado de colostro forneceu 241% da recomendação dietética e o de leite de transição atingiu 66%. CONCLUSÕES: o grupo de mulheres estudadas apresentou um estado nutricional satisfatório de vitamina E, refletido no leite materno, principalmente no colostro, cujos valores foram capazes de suprir mais do que o dobro do requerimento nutricional do lactente.OBJECTIVES: to evaluate levels of alpha-tocopherol in the serum and breast-milk of women at various stages in lactation and to confirm whether nutritio nally appropriate levels of vitamin E are present in the milk given to the babies. METHODS: thirty-two child-bearing women with an average age of 25 years took part in the study. 5 mL of blood and 2 mL of colostrum were collected, under fasting conditions, for the purposes of analyzing the levels of alpha-tocopherol. Between 10 to 15 days after childbirth, a further 2 mL of breast-milk was collected. The samples were

  18. Teores de retinol, beta-caroteno e alfa-tocoferol em leites bovinos comercializados na cidade de São Paulo Amounts of retinol, beta-carotene and alpha-tocopherol in cow milk comercialized in the city of São Paulo

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    Rute BIANCHINI

    1999-12-01

    Full Text Available Os teores de retinol, beta-caroteno e alfa-tocoferol foram determinados por cromatografia líquida de alta eficiência em leites em pó, pasteurizados e esterilizados, comercializados na Cidade de São Paulo. Após a saponificação e extração, os compostos foram determinados simultaneamente utilizando-se coluna de sílica, fase móvel constituída por hexano:isopropanol (99:1 e fluxo de 2,0mL/min. O retinol e o beta-caroteno foram determinados no detector UV/visível e o alfa-tocoferol no detector de fluorescência, ligado em série com o anterior. Os valores de vitamina A dos leites foram calculados com e sem a consideração do beta-caroteno. A maior contribuição deste nutriente no valor de vitamina A esteve entre os leites em pó, cerca de 17% em uma das marcas. Os altos teores das vitamina A e E encontrados em alguns leites, indicam que os mesmos provavelmente receberam adição destas vitaminas, não trazendo, entretanto, tal informação no rótulo. A análise de vitaminas nestes produtos indica a necessidade de maior controle de qualidade dos mesmos.The amount of retinol, beta-carotene, alpha -tocopherol in powder, pasteurized and sterilized milk, comercialized in the city of São Paulo, were analyzed by high performance liquid chromatography. After saponification and extraction, compounds were determined simultaneously through a normal-phase column, mobile phase composed by hexan:2-propanol (99:1 and 2 mL/min flow. The retinol and beta-carotene were analysed by a UV/visible detector and the alpha-tocopherol by a fluorescence detector, both linked in series. The milk vitamin A values were calculated with and without beta-carotene. The major contribution of beta-carotene in the vitamin A value was in powder milks, around 17% in one of the brands. The high amounts of vitamin A and E found in some milks indicate that they probably were enriched with these vitamins but nothing is mentioned about this in their labels. The analysis of

  19. 聚乙二醇干扰素α-2a与干扰素α-2a治疗慢性丙型肝炎的短期疗效分析%Short-term curative effect of ribavirin combination therapy with pegylated interferon alfa-2a vs.interferon alfa-2a in patients with chronic hepatitis C

    Institute of Scientific and Technical Information of China (English)

    王盟; 郑伟阳; 张红宇; 李志勤; 蒋冬; 刘艳红; 周蓉; 李晓刚; 张英英

    2014-01-01

    Objective To perform a retrospective cohort study in order to determine the differences in short-term curative effect of ribavirin in combination with interferon alfa (IFNα)-2a vs.pegylated (Peg)-IFNα-2a in patients with chronic hepatitis C (CHC).Methods One-hundred-and-eighty-eight treatmentna(i)ve CHC patients who were administered combination therapy of ribavirin with IFNα from 2010 to 2012.One-hundred-and-thirty-three of the patients received the therapy with IFNα-2a and the remaining 55 received Peg-IFNα-2a.Hepatitis C virus (HCV) load and levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured at treatment weeks 4,12,24,and 48.Adverse reactions were recorded.Differences between the groups were assessed by statistical analysis.Results The patients in the Peg-IFNα-2a group and the IFNα-2a group showed no significant difference in sex distribution,age,smoking habits,or drinking habits at baseline (all P > 0.05).Both antiviral therapies significantly reduced the HCV load and levels of ALT and AST (baseline levels vs.all treatment weeks examined,P < 0.05); however,the reduction in the HCV load at week 4 was significantly more robust with the Peg-IFNα-2a therapy ((2.96 ± 0.66) log10 IU/ ml vs.(3.47 ± 1.42)1og10 IU/ml; F =4.14,P =0.04).The Peg-IFNα-2a group also showed a significant higher rate of rapid virological response (RVR) than the IFNα-2a group (72.72% vs.57.14%;x2 =4.37,P =0.04),but there were no statistically significant differences found between the two groups for early virological response rate (EVR),endpoint antiviral treatment virologic response rate (ETR),biochemical response rate,or rate of adverse reactions (all P > 0.05).Conclusion Ribavirin in combination with Peg-IFNα-2a produces a better RVR than in combination with IFNα-2a.Yet,the EVR,ETR,biochemical response rate,and rate of adverse reactions is similar for the two forms of IFNα-2a,Further studies are required to determine the

  20. Potential cost saving of Epoetin alfa in elective hip or knee surgery due to reduction in blood transfusions and their side effects: a discrete-event simulation model.

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    Jörg Tomeczkowski

    Full Text Available OBJECTIVES: Transfusion of allogeneic blood is still common in orthopedic surgery. This analysis evaluates from the perspective of a German hospital the potential cost savings of Epoetin alfa (EPO compared to predonated autologous blood transfusions or to a nobloodconservationstrategy (allogeneic blood transfusion strategyduring elective hip and knee replacement surgery. METHODS: Individual patients (N = 50,000 were simulated based on data from controlled trials, the German DRG institute (InEK and various publications and entered into a stochastic model (Monte-Carlo of three treatment arms: EPO, preoperative autologous donation and nobloodconservationstrategy. All three strategies lead to a different risk for an allogeneic blood transfusion. The model focused on the costs and events of the three different procedures. The costs were obtained from clinical trial databases, the German DRG system, patient records and medical publications: transfusion (allogeneic red blood cells: €320/unit and autologous red blood cells: €250/unit, pneumonia treatment (€5,000, and length of stay (€300/day. Probabilistic sensitivity analyses were performed to determine which factors had an influence on the model's clinical and cost outcomes. RESULTS: At acquisition costs of €200/40,000 IU EPO is cost saving compared to autologous blood donation, and cost-effective compared to a nobloodconservationstrategy. The results were most sensitive to the cost of EPO, blood units and hospital days. CONCLUSIONS: EPO might become an attractive blood conservation strategy for anemic patients at reasonable costs due to the reduction in allogeneic blood transfusions, in the modeled incidence of transfusion-associated pneumonia andthe prolongedlength of stay.

  1. Acute inflammatory demyelinating polyneuropathy after treatment with pegylated interferon alfa-2a in a patient with chronic hepatitis C virus infection: a case report

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    Lahbabi Mounia

    2012-09-01

    Full Text Available Abstract Introduction The combination of polyethylene glycol (PEGylated interferon (pegylated interferon and ribavirin has been shown to be an effective treatment for chronic hepatitis C virus. In general, common side effects related to this combination therapy are mild and are well tolerated. However, peripheral neuropathy including demyelinating polyneuropathy related to PEG-interferon α2a (pegylated interferon alfa-2a is extremely rare. In the literature, only one case of acute inflammatory demyelinating polyneuropathy related to PEG-interferon α2a has been published previously. Case presentation To the best of our knowledge we present only the second case of acute inflammatory demyelinating polyneuropathy related to PEG-interferon α2a, occurring in a 63-year-old Caucasian man. He developed tingling, numbness, and weakness of his upper and lower extremities with acute neurological deficits after five weeks of a combination therapy with PEG-interferon α2a and ribavirin for chronic hepatitis C virus infection. His clinical course, neurological findings, and his electromyogram results were all consistent with acute inflammatory demyelinating polyneuropathy. Our patient recovered completely after interferon was stopped and symptomatic treatment and a further electromyogram showed a disappearance of neuropathy. Four weeks later, PEG-interferon α2a was reintroduced with a gradually increasing dose without any reappearance of neurological symptoms allowing hepatitis C seroconversion. Conclusions Recognition of this rare yet possible presentation is important for early and accurate diagnosis and treatment. This case report also suggests that the reintroduction of PEGylated interferon in patients who had presented with acute inflammatory demyelinating polyneuropathy related to interferon α may be safe, but this must be confirmed by further studies.

  2. Análisis cuantitativo de cuarzo-alfa en mezclas de cemento portland anhidro y calcita sintética por difracción de rayos X con equipo Philips PW-1700

    OpenAIRE

    Sagrera-Moreno, J. L.

    1985-01-01

    In this article there are explained the necessary steps to make up a quantitative analysis of alpha-quartz applying the technique of rays X diffraction, in artificial mixtures of anhydrous portland cement and synthetic calcite, which contents are known.

    En el presente artículo se explican los pasos necesarios para realizar un análisis cuantitativo de cuarzo-alfa aplicando la técnica de difracción de rayos X en mezclas artificiales de cemento portland anhidro y calcita sinté...

  3. Análisis cuantitativo de cuarzo-alfa en mezclas de cemento portland anhidro y calcita sintética por difracción de rayos X con equipo Philips PW-1700

    Directory of Open Access Journals (Sweden)

    Sagrera-Moreno, J. L.

    1985-09-01

    Full Text Available In this article there are explained the necessary steps to make up a quantitative analysis of alpha-quartz applying the technique of rays X diffraction, in artificial mixtures of anhydrous portland cement and synthetic calcite, which contents are known.

    En el presente artículo se explican los pasos necesarios para realizar un análisis cuantitativo de cuarzo-alfa aplicando la técnica de difracción de rayos X en mezclas artificiales de cemento portland anhidro y calcita sintética, cuyos contenidos son conocidos.

  4. El receptor de estrógenos alfa como mediador del efecto proliferativo de progestágenos en cáncer de mama The role of estrogen receptor alpha in breast cancer cell proliferation mediated by progestins

    OpenAIRE

    Sebastián Giulianelli; Vaqué, José P.; Victoria Wargon; Rocío Soldati; Vanzulli, Silvia I; Rubén Martins; Eduardo Zeitlin; Luisa Helguero; Caroline Lamb; Molinolo, Alfredo A.; J Silvio Gutkind; Claudia Lanari

    2012-01-01

    En carcinomas mamarios murinos (C4-HD) y en células de cáncer de mama humano (T47D) observamos que el progestágeno sintético, acetato de medroxiprogesterona (MPA), induce la activación del receptor de estrógenos alfa (REa) y su asociación nuclear con el receptor de progesterona (RP). En este trabajo postulamos que dicha interacción a nivel genómico sería fundamental para desarrollar respuestas proliferativas mediadas por progestágenos. Demostramos que el antiestrógeno fulvestrant (FUL, ICI182...

  5. Análise de crédito por meio de modelos de previsão de insolvência: um estudo de caso na Empresa Cerâmica Alfa S.A. = Credit analysis through models for the forcasting of insolvency of the company Cerâmica Alfa S.A.

    Directory of Open Access Journals (Sweden)

    Paula Bez Birolo

    2011-07-01

    Full Text Available O objetivo deste artigo é avaliar os benefícios da utilização de modelos de previsão de insolvência para a análise de crédito em uma empresa do setor cerâmico. É descritivo, de caráter quantitativo, realizado por meio de um estudo de caso na empresa Cerâmica ALFA S.A. Para execução da pesquisa selecionou-se intencionalmente seis clientes, de acordo com os seguintes dados: a informações creditícias disponíveis no sistema gerencial de crédito/cobrança e b demonstrações contábeis divulgadas nos sites dos respectivos clientes. Os resultados apontam que os modelos de Elizabetsky e Kanitz demonstram fragilidades em identificar a situação financeira dos clientes. O modelo de previsão de insolvência desenvolvido por Matias apresenta melhor desempenho, em relação aos anteriores, para a análise de crédito. Conclui-se que é vantajoso utilizar o modelo de Matias para a análise de crédito, pois ele é adequado às necessidades da empresa e serve como um instrumento para auxílio na concessão de crédito e tomada de decisão. The objective of this article is to assess the benefits of using insolvency forecasting models for the credit analysis of a company in the ceramic sector. This study is descriptive of a quantitative nature through a case study in Cerâmica ALFA S.A. Six clients with the following data were intentionally selected for the execution of the research: a credit information available in the credit/collection management system and b account statements divulged in the sites of the respective clients. The results show that the Elizabetsky and Kanitz models demonstrated fragility in identifying the financial situation of the clients. The insolvency forecasting model developed by Matias showed better performance in relation to the previous ones for credit analysis. It was concludrd that it is advantageous to use the Matias model for credit analysis, because it is suitable for the company’s requirements and serves

  6. The HI Mass Function and Velocity Width Function of Void Galaxies in the Arecibo Legacy Fast ALFA Survey

    CERN Document Server

    Moorman, Crystal M; Hoyle, Fiona; Pan, Danny C; Haynes, Martha P; Giovanelli, Riccardo

    2014-01-01

    We measure the HI mass function (HIMF) and velocity width function (WF) across environments over a range of masses $7.2<\\log(M_{HI}/M_{\\odot})<10.8$, and profile widths $1.3\\log(km/s)<\\log(W)<2.9\\log(km/s)$, using a catalog of ~7,300 HI-selected galaxies from the ALFALFA Survey, located in the region of sky where ALFALFA and SDSS (Data Release 7) North overlap. We divide our galaxy sample into those that reside in large-scale voids (void galaxies) and those that live in denser regions (wall galaxies). We find the void HIMF to be well fit by a Schechter function with normalization $\\Phi^*=(1.37\\pm0.1)\\times10^{-2} h^3Mpc^{-3}$, characteristic mass $\\log(M^*/M_{\\odot})+2\\log h_{70}=9.86\\pm0.02$, and low-mass-end slope $\\alpha=-1.29\\pm0.02$. Similarly, for wall galaxies, we find best-fitting parameters $\\Phi^*=(1.82\\pm0.03)\\times10^{-2} h^3Mpc^{-3}$, $\\log(M^*/M_{\\odot})+2\\log h_{70}=10.00\\pm0.01$, and $\\alpha=-1.35\\pm0.01$. We conclude that void galaxies typically have slightly lower HI masses than ...

  7. Effect of short-term ornithine alpha-ketoglutarate pretreatment on intestinal ischemia-reperfusion in rats Efeitos do pré-tratamento em curto prazo com ornitina alfa-cetoglutarato na isquemia-reperfusão intestinal em ratos

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    Eduardo Silvio Gouveia Gonçalves

    2011-01-01

    Full Text Available PURPOSE: To investigate the effects of preventive enteral administration of ornithine alpha-ketoglutarate (OKG in an ischemia-reperfusion rat model. METHODS: Sixty rats were randomized into five groups (G1-G5, n = 12. Each group was divided into two subgroups (n = 6 and treated with calcium carbonate (CaCa or OKG by gavage. Thirty minutes later, the animals were anesthetized with xylazine 15mg + ketamine 1mg ip and subjected to laparotomy. G1-G3 rats served as controls. Rats in groups G4 and G5 were subjected to ischemia for 30 minutes. Ischemia was achieved by clamping the small intestine and its mesentery, delimiting a segment of bowel 5 cm long and 5 cm apart from the ileocecal valve. In addition, G5 rats underwent reperfusion for 30 minutes. Blood samples were collected at the end of the laparotomy (G1, after 30 minutes (G2, G4 and 60 minutes (G3, G5 to determine concentrations of metabolites (pyruvate, lactate, creatine phosphokinase (CPK, thiobarbituric acid reactive substances (TBARS and glutathione (GSH. RESULTS: There was a significant decrease in tissue pyruvate and lactate and plasma CPK levels in OKG-treated rats at the end of reperfusion period. GSH levels did not change significantly in ischemia and reperfusion groups. However, TBARS levels increased significantly (pOBJETIVO: Investigar os efeitos da administração enteral preventiva de ornitina alfa-cetoglutarato (OKG em modelo de isquemia-reperfusão no rato. MÉTODOS: Sessenta ratos foram randomizados em cinco grupos (G1-G5, n=12. Cada grupo foi redistribuído em dois subgrupos (n=6 e tratado com carbonato de cálcio (CaCa ou OKG por gavagem. Trinta minutos mais tarde, os animais foram anestesiados com xilazina 1mg+cetamina 15mg i.p. e submetidos à laparotomia. Os ratos dos grupos G4-G5 foram submetidos à isquemia por 30 minutos. A isquemia foi obtida por pinçamento do intestino delgado, delimitando um segmento com 5 cm de comprimento e distando 5 cm da válvula ileocecal. O

  8. El receptor de estrógenos alfa como mediador del efecto proliferativo de progestágenos en cáncer de mama The role of estrogen receptor alpha in breast cancer cell proliferation mediated by progestins

    Directory of Open Access Journals (Sweden)

    Sebastián Giulianelli

    2012-08-01

    Full Text Available En carcinomas mamarios murinos (C4-HD y en células de cáncer de mama humano (T47D observamos que el progestágeno sintético, acetato de medroxiprogesterona (MPA, induce la activación del receptor de estrógenos alfa (REa y su asociación nuclear con el receptor de progesterona (RP. En este trabajo postulamos que dicha interacción a nivel genómico sería fundamental para desarrollar respuestas proliferativas mediadas por progestágenos. Demostramos que el antiestrógeno fulvestrant (FUL, ICI182.780 indujo la regresión completa de tumores C4-HD creciendo con MPA. El progestágeno indujo la expresión temprana de CCND1 y MYC en células T47D y este efecto fue revertido al bloquear el REa. En células tratadas con MPA utilizamos ensayos de inmunoprecipitación de la cromatina (ChIP y corroboramos la colocalización nuclear de RP/REa en los mismos sitios de los promotores de CCND1 y MYC. El ICI no afectó la unión de RP a ambas secuencias regulatorias, pero sí inhibió la unión del REa. Confirmamos la interacción nuclear entre REa y RP en muestras de cáncer de mama humano. Los resultados demuestran que la presencia del REa, interactuando con el RP, en promotores de CCND1 y MYC es fundamental para la transcripción génica y la proliferación celular inducida por el progestágeno.In C4-HD murine mammary carcinomas and in human breast cancer T47D cells, we showed that medroxyprogesterone acetate (MPA induces a nuclear physical association between estrogen receptor alpha (ERa and progesterone receptors (PR. The blockade of ERa inhibits cell proliferation mediated by progestins. We hypothesized that this nuclear association between ERa/PR is necessary to trigger progestin-induced cell proliferation and tumor growth. We demonstrated that fulvestrant (FUL, ICI182.780 induced complete regression of C4-HD tumors growing with progestins. MPA treatment induced an early increase in both CCND1 and MYC expression in T47D cells. The blockade of ERa

  9. Cost effectiveness of peginterferon alfa-2B combined with ribavirin for the treatment of chronic hepatitis C in Brazil

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    Marcelo Cunio Machado Fonseca

    2009-06-01

    Full Text Available The treatment of chronic hepatitis C (CHC with peginterferon alpha-2b/ribavirin (PegIFN + Rib produced larger sustained viral response (SVR compared to the conventional (non-pegylated interferon/ribavirin (IFN + Rib, but its cost-effectiveness was not assessed in Brazil. We developed a Markov model to mirror the natural disease history and cohorts of patients with hepatitis C virus (HCV, that received PegIFN + Rib or IFN + Rib treatment for 48 or 24 weeks, according to viral genotype and liver histology. The SVRs for the treatments PegIFN + Rib and IFN + Rib were respectively 48% and 34% (genotype 1, and 88% and 80% (genotype non-1. Three Delphi panels were conducted with hepatologists and intensivists, and another one with oncologists. The costs are expressed in 2006 Brazilian Reais (R$ and the benefits were discounted at 3%. In genotype 1 HCV patients, PegIFN + Rib increases the life expectancy (LE in 0.51 year, and the quality-adjusted life years (QALY in 0.78, as compared to IFN + Rib. In genotype non-1 HCV patients, PegIFN + Rib increases the LE in 0.29 years and the QALY in 0.44 years, as compared to IFN + Rib. The incremental cost-effectiveness rate, considering all the genotypes, was of R$19,848.34 per QALY. Peginterferon alpha-2b with ribavirin is a cost-effective therapy for the treatment of naïve CHC adult patients compared to the interferon alpha-2b and ribavirin regime, irrespective of the viral genotype.

  10. El ácido alfa lipoico y su poder antioxidante frente al cáncer y las patologías de sensibilización central

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    Marisa Durand

    2013-08-01

    Full Text Available Introducción: El ácido alfa lipoico (ALA puede controlar y limitar la cantidad de radicales libres, influyendo el desarrollo de patologías como el cáncer o las enfermedades de sensibilización central, aunque los mecanismos moleculares implicados en este proceso aún están dilucidándose. Objetivo: Reunir y contrastar información sobre las propiedades antioxidantes del ALA en la prevención y desarrollo de las patologías relacionadas con el estrés oxidativo. Material y métodos: En este trabajo, se analizan más de 100 artículos publicados en los últimos 20 años que relacionan el consumo de ALA y la prevalencia y desarrollo de patologías relacionadas con el estrés oxidativo. Los artículos han sido obtenidos en diferentes bases de datos (PubMed central, Web of Science, Elsevier Journal, Science Direct, e incluyen experimentos en células, animales y humanos. Las palabras clave utilizadas fueron: cáncer, enfermedades de sensibilización central, radicales libres, y ALA. Resultados y discusión: Se han reunido resultados de trabajos realizados in vitro y en animales de laboratorio en los que se pone de manifiesto el efecto del ALA en el control de la apoptosis celular de diferentes tipos de cánceres mediante un aumento de las especies reactivas de oxígeno, así como también el retardo en el crecimiento de las mismas. Aparte, se ha demostrado que la capacidad antioxidante del ALA y su potencial para regenerar otros antioxidantes es de gran importancia para tratar las patologías de sensibilización central. Conclusiones: El ALA ha demostrado un papel significativo como antioxidante y prooxidante en el cáncer y las patologías de sensibilización central, aunque son necesarias más investigaciones en humanos.

  11. Study on the expression of HLA-DR/CD69 on CD4 +/CD8 + T cells in the treatment of HBeAg-positive chronic hepatitis B with interferon alfa-2b%干扰素α-2b治疗HBeAg阳性慢性乙型肝炎患者CD4+/CD8+T细胞活化分子CD69和HLA-DR的表达

    Institute of Scientific and Technical Information of China (English)

    石庆凤; 宋吉奎; 张颖新; 袁建国; 王思奎; 李庆方; 赵红奎

    2011-01-01

    目的 观察HBeAg阳性慢性乙型肝炎患者干扰素α-2b治疗前、中、后期CD4+/CD8+T细胞的活性与疗效的相关性.方法 32例患者隔日1次干扰素5 MU肌注,共24周.抽取干扰素α-2b治疗前,治疗1、4、12、24周的外周静脉血,密度梯度离心法分离获得外周血单个核细胞(PBMC),流式细胞仪检测CD4+/CD8+T细胞比例及CD4+/CD8+T细胞表面CD69、HLA-DR的表达.结果 干扰素α-2b治疗结束,HBV DNA含量转阴者12例(37%),其中HBeAg、抗-HBe血清转换5例(16%);12例治疗无效(37%)及8例HBV DNA抑制(25%).HBV DNA转阴者治疗前,治疗1、4周时CD69、HLA-DR表达率较治疗无效及HBV DNA抑制者高,差异有统计学意义(P<0.05).HBV DNA转阴者治疗后1周时,CD69、HLA-DR表达率最高;4周时CD4+/CD8+T细胞的CD69、HLA-DR表达率较前下降,但仍保持较高表达率;12周时CD69、HLA-DR表达率较前更下降,与治疗无效及HBV DNA抑制者相比差异无统计学意义(P>0.05);24周时CD69、HLA-DR表达率最低,与治疗无效及HBV DNA抑制者比较差异有统计学意义(P<0.05).结论 HBeAg阳性慢性乙型肝炎患者CD4+/CD8+T细胞活化分子CD69、HLA-DR的表达,对干扰素α-2b的疗效有预测作用.%Objective To study the efficacy prediction and the activation of CD4 + /CD8 + T cells in the treatment of HBeAg - positive chronic hepatitis B with interferon alfa -2b. Methods 32 patients were given interferon alfa -2b 5 MU every other day for 24 weeks. PBH|C were isolated from fresh peripheral blood of chronic severe hepatitis B patients by Ficoll - Hypaque density gradient centrifugation and cultured with plastic - adherence method. FACS was used to analyze the expression of the CD4 + /CD8 + T cells surface markers, including human leukocyte antigen - DR ( HLA - DR) and CD69. Results At the end of treatment, HBV - DNA levels loss rates were 37% (12 cases) , including HBeAg seroconversion in 5 cases(16% ). In 8 cases(25% ) HBV - DNA

  12. Drug: D03305 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D03305 Drug Interferon alfa (BALL-1) (JAN); Interferon alfa (NAMAWA) (JAN); Interferon...logic organisms and parasites 63 Biological preparations 639 Miscellaneous 6399 Others D03305 Interferon alfa (BALL-1) (JAN); Interfe...ron alfa (NAMAWA) (JAN); Interferon alfa (INN) Anatomical Therapeutic Chemical (ATC...MUNOSTIMULANTS L03A IMMUNOSTIMULANTS L03AB Interferons L03AB01 Interferon alfa natural D03305 Interferon alfa (BALL-1) (JAN); Interfe...ron alfa (NAMAWA) (JAN); Interferon alfa (INN) Target-based classification of drugs

  13. Arecibo Pulsar Survey Using ALFA. IV. Mock Spectrometer Data Analysis, Survey Sensitivity, and the Discovery of 41 Pulsars

    CERN Document Server

    Lazarus, P; Hessels, J W T; Karako-Argaman, C; Kaspi, V M; Lynch, R; Madsen, E; Patel, C; Ransom, S M; Scholz, P; Swiggum, J; Zhu, W W; Allen, B; Bogdanov, S; Camilo, F; Cardoso, F; Chatterjee, S; Cordes, J M; Crawford, F; Deneva, J S; Ferdman, R; Freire, P C C; Jenet, F A; Knispel, B; Lee, K J; van Leeuwen, J; Lorimer, D R; Lyne, A G; McLaughlin, M A; Siemens, X; Spitler, L G; Stairs, I H; Stovall, K; Venkataraman, A

    2015-01-01

    The on-going PALFA survey at the Arecibo Observatory began in 2004 and is searching for radio pulsars in the Galactic plane at 1.4 GHz. Observations since 2009 have been made with new wider-bandwidth spectrometers than were previously employed in this survey. A new data reduction pipeline has been in place since mid-2011 which consists of standard methods using dedispersion, searches for accelerated periodic sources, and search for single pulses, as well as new interference-excision strategies and candidate selection heuristics. This pipeline has been used to discover 41 pulsars, including 8 millisecond pulsars (MSPs; P = 100 ms that gradually becomes up to a factor of ~10 worse for P > 4 s at DM < 150 pc/cc. This degradation of sensitivity at long periods is largely due to red noise. We find that 35 +- 3% of pulsars are missed despite being bright enough to be detected in the absence of red noise. This reduced sensitivity could have implications on estimates of the number of long-period pulsars in the Gal...

  14. IFNα与其他治疗方法联合应用%Interferon-alfa-based Combination Therapy

    Institute of Scientific and Technical Information of China (English)

    童葵塘

    2007-01-01

    IFNα是治疗病毒性疾病和某些肿瘤的重要制品,为获得更好的效果,目前临床上大多采取与其他疗法联合应用的方法.IFNα与胸腺素α1联合治疗慢性乙型肝炎能提高疗效,尤其是远期效果.IFNα与利巴韦林联合治疗慢性丙肝优于单用一种药物已为国内外确认.至于肿瘤治疗方面,黑素瘤和肾癌手术切除后用IFNα治疗可显著延长无复发生存期和总生存期.IFNα与细胞毒性化疗药物联合应用可提高淋巴瘤的治疗效果.本文对IFNα作为免疫佐剂的应用也作了介绍.

  15. CONSORT: Effects of adding adefovirdipivoxil to peginterferon alfa-2a at different time points on HBeAg-positivepatients

    Science.gov (United States)

    Zhang, Ka; Cao, Hong; Liang, Jiayi; Shu, Xin; Sun, Haixia; Li, Gang; Xu, Qihuan

    2016-01-01

    Abstract Background: The aims of this study were to compare the efficacy and safety of the addition of adefovir dipivoxil (ADV) (started at different time points) to pegylated interferon alpha-2a (PEG-INF-α2a) and PEG-INF-α2a monotherapy. This prospective, randomized study sought to evaluate the safety and efficacy of the combination of PEG-INF-α2a and ADV at different time points.120 patients were randomized into groups that received PEG-INF-α2a as monotherapy (group A) or in combination with ADV started at week 0 (group B), 12 (group C), or 24 (group D). All patients were followed for 48 weeks. Efficacy and safety analyses were performed. Methods: Patients in group a received 135 μg of PEG-INF-α2a by subcutaneous injection once weekly for 48 weeks. Patients in the ADV add-on group received 135 μg of PEG-INF-α2a subcutaneously once weekly and received 10 mg of ADV administered once daily for 48 weeks. HBV DNA, HBsAg, HBeAg, and hepatitis B e antibody levels were determined. Responses were determined at week 12 (ADV add-on), the end of treatment for PEG-INF-α2a (48weeks) and ADV (EOT) and at the end of 96 weeks of follow-up (EOF). Results: The rate of HBV DNA loss were higher in the combination groups than group A at the week 12, week 48, the EOT and EOF (P 0.05). The alanineaminotransferase (ALT) normalization rate was higher in the combination group than group A only at the EOT (P = 0.007). By the EOF, the patients with ADV added at week 12 achieved higher rates of HBV DNA loss (71.9%), HBeAg seroconversion (50.0%), HBsAg loss (15.6%), and ALT normalization (78.1%). Conclusions: PEG-INF-α2a plus ADV combination therapy is safe and superior to PEG-INF-α2amonotherapyfor decreasing serum HBV DNA and normalizing the ALT level but has no significant impact on the rate of HBeAg seroconversion and HBsAg loss. Adding ADV at week 12 may be an optimal combination strategy. PMID:27495085

  16. Norepinephrine Causes a Biphasic Change in Mammalian Pinealocye Membrane Potential: Role of alfa1B-Adrenoreceptors, Phospholipase C, and Ca2plus

    Czech Academy of Sciences Publication Activity Database

    Zemková, Hana; Stojilkovič, S.S.; Klein, D. C.

    2011-01-01

    Roč. 152, č. 10 (2011), s. 3842-3851. ISSN 0013-7227 R&D Projects: GA ČR(CZ) GA305/07/0681 Institutional research plan: CEZ:AV0Z50110509 Keywords : Ca2plus-activated Kplus channels * voltage-gated Ca2plus channels * electrical activity * pineal ocyte Subject RIV: ED - Physiology Impact factor: 4.459, year: 2011

  17. PERCEPCIÓN DEL IMPACTO DE LA APLICACIÓN DE MECANISMOS DE ASEGURAMIENTO DE LA CALIDAD EN LA EDUCACIÓN SUPERIOR EN COSTA RICA: PROYECTO ALFA-CINDA-UNIVERSIDAD DE COSTA RICA (PERCEIVED IMPACT OF QUALITY ASSURANCE MECHANISMS ON COSTA-RICAN HIGHER EDUCATION: THE ALFA-CINDA-UNIVERSIDAD-DE-COSTA-RICA PROJECT

    Directory of Open Access Journals (Sweden)

    Guido Guido, Elsiana

    2012-05-01

    Full Text Available Resumen: En este artículo se presentan las acciones realizadas dentro del Proyecto Alfa Nro. DCl-ALA 2008/42: Aseguramiento de la calidad: políticas públicas y gestión universitaria financiado con fondos de la Unión Europea y coordinado, de manera general, por el Centro Interuniversitario de Desarrollo, CINDA. Participan 16 universidades latinoamericanas y 7 universidades europeas, y el objetivo general del proyecto es mejorar la gestión de la calidad de la Educación Superior, tanto a nivel de los sistemas nacionales como de las instituciones de Educación Superior, y contribuir con la generación de conocimiento y confianzas mutuas acerca de la calidad de la Educación Superior entre los países latinoamericanos y europeos. Como parte de las actividades desarrolladas se llevaron a cabo estudios en 7 países (5 de Latinoamérica y 2 de Europa para evaluar el impacto de los procesos de aseguramiento de la calidad implementados en las universidades. En Costa Rica, el proyecto ha sido coordinado y ejecutado por la Universidad de Costa Rica desde el año 2009 hasta la fecha. El estudio se realizó en dos universidades estatales y dos privadas, durante el período comprendido entre octubre de 2010 y mayo de 2011, se realizaron 26 entrevistas presenciales y 8 sesiones de grupo a las diferentes poblaciones definidas en el estudio, además, se realizaron un total de 652 encuestas a estudiantes activos y a personas egresadas. Los principales resultados obtenidos en Costa Rica se resumen en este artículo, de acuerdo con los niveles y dimensiones de calidad que fueron definidos en el marco referencial del proyecto.Abstract: The article presents actions carried out in Costa Rica, in the frame of the project Alfa Nro. DCl-ALA 2008/42: Quality assurance: public policies and management in universities. This project is funded by the European Commission and its general coordination is in charge of the Inter-university Center for Development (CINDA, in

  18. A taxa de resposta sustentada da hepatite C crônica ao tratamento com os diversos interferons-alfa e ribavirinas distribuídos pelo governo brasileiro é semelhante à da literatura mundial The sustained response rates for chronic hepatitis C patients undergoing therapy with the several interferons and ribavarins supplied by Brazilian’s Health Ministry is comparable to those reported in the literature

    Directory of Open Access Journals (Sweden)

    Rafael Nastás Acras

    2004-03-01

    Full Text Available RACIONAL: As taxas de resposta sustentada aos interferons-alfa 2A e 2B associados à ribavirina são descritas na literatura como sendo de 40%. No entanto, a taxa de resposta sustentada em ambiente de freqüentes mudanças dos produtos fornecidos ainda não era conhecida. OBJETIVOS: Avaliar a prevalência de resposta sustentada e os fatores preditivos de melhor resposta ao tratamento da hepatite C crônica, com as diferentes combinações e procedências de interferons-alfa e ribavirina, fornecidos pelo Ministério da Saúde e compará-la às da literatura mundial. CASUÍSTICA E MÉTODOS: Foi realizado estudo de coorte, não-controlado, em 87 pacientes do ambulatório de Hepatologia do Hospital de Clínicas da Universidade Federal do Paraná, Curitiba, PR, tratados com interferon-alfa e ribavirina, entre agosto de 1999 e agosto de 2002. Avaliou-se a prevalência de resposta sustentada e sua correlação com sexo feminino, idade, genótipos 2 e 3 e graus de fibrose. RESULTADOS: Houve taxa de resposta sustentada em 32,1% dos pacientes, correlacionada positivamente com os genótipos 2 e 3. CONCLUSÕES: As taxas de resposta sustentada encontradas são comparáveis com as da literatura internacional, apesar das freqüentes variações quanto ao tipo e/ou procedência dos produtos.BACKGROUND: The sustained response rates of chronic hepatitis C therapy to interferons alpha 2A and 2B associated to ribavirin are described as approximately 40%. Nevertheless, studies on the sustained response rates in a setting of frequent changes of type of interferon as well as of supplies of interferons and ribavirin are lacking. AIMS: To evaluate the prevalence of sustained response and to determine the predictors of response to chronic hepatitis C therapy, in a cohort of outpatients at Curitiba, PR, south Brazil, in a setting of heterogeneous therapy regarding type and supply of the medications. POPULATION AND METHODS: A cohort of 87 chronic hepatitis C patients

  19. Níveis de alfa-tocoferol no soro e colostro de lactantes e associação com variáveis maternas Alpha-tocopherol level in serum and colostrum of breastfeeding women and association with maternal variables

    Directory of Open Access Journals (Sweden)

    Larissa Queiroz de Lira

    2012-08-01

    Full Text Available OBJETIVOS: Diagnosticar bioquimicamente o estado nutricional de vitamina E de lactantes por meio da análise do alfa-tocoferol no soro e no colostro, verificar sua associação com variáveis maternas e determinar a prevalência de deficiência de vitamina E nessas mulheres. MÉTODOS: Participaram do estudo 103 puérperas que foram classificadas quanto às seguintes variáveis maternas: idade, estado nutricional pré-gestacional, ganho de peso gestacional, paridade e tipo de parto. Amostras de soro e colostro foram coletadas em jejum no pós-parto imediato e o alfa-tocoferol foi analisado por cromatografia líquida de alta eficiência (CLAE. Para definir o estado nutricional de vitamina E, foi adotado ponto de corte sérico (697,7 μg/dL. A análise estatística foi realizada com o uso do teste t de Student para amostras independentes e correlação de Pearson. As diferenças foram consideradas significativas quando pPURPOSE: To determine the nutritional status of vitamin E in breastfeeding women through the analysis of alpha-tocopherol concentration in serum and colostrum, to analyze its relation with maternal variables and to determine the prevalence of vitamin E deficiency in these women. METHODS: The study included 103 mothers who were classified according to maternal variables: age, nutritional status before pregnancy, gestational weight gain, parity and mode of delivery. Colostrum and serum samples were collected under fasting conditions in the immediate postpartum period. Alpha-tocopherol was analyzed by high performance liquid chromatography (HPLC. A serum cutoff of 697.7 μg/dL was adopted to define the nutritional status of vitamin E. Statistical analysis was performed with the Student's t test for independent samples and Pearson's correlation. Differences were significant when p<0.05. RESULTS: The average concentration of alpha-tocopherol was 1.125±551.0 μg/dL in colostrum and 1,138.6±346.0 μg/dL in serum, indicating adequate

  20. Tratamento de pacientes com hepatite crônica pelo vírus C com interferon-alfa e ribavirina: a experiência da Secretaria de Saúde do Rio Grande do Sul Interferon-alpha and ribavirin therapy on chronic hepatitis C virus infection: the experience of Rio Grande do Sul State Health Department, Brazil

    Directory of Open Access Journals (Sweden)

    Alexandro Vaesken Alves

    2003-12-01

    Full Text Available RACIONAL: A infecção crônica pelo vírus da hepatite C é importante problema de saúde pública. OBJETIVOS: Analisar os resultados do tratamento combinado interferon-alfa/ribavirina e identificar fatores preditivos de resposta em pacientes adultos com hepatite crônica C de um programa público de fornecimento de medicamentos. PACIENTES E MÉTODOS: Estudo retrospectivo de registros consecutivos de 400 pacientes com hepatite crônica C tratados com interferon/ribavirina em programa estabelecido pela Secretaria Estadual de Saúde do Rio Grande do Sul, Porto Alegre, RS, no período de 1999 a 2000. RESULTADOS: A distribuição entre homens e mulheres foi similar, e a média de idade foi de 46,5 ± 10,3 anos. Observou-se resposta ao final do tratamento e resposta sustentada em 49% e 32% dos pacientes, respectivamente. Resposta sustentada foi significativamente maior nas mulheres e em pacientes infectados com genótipos não-1, não se tendo observado diferença quando avaliada a idade e o grau de atividade e estágio histológico. CONCLUSÃO: Em pacientes adultos com hepatite crônica C, a resposta ao tratamento combinado interferon-alfa/ribavirina foi observada em um terço deles. Taxas maiores de resposta foram observadas em mulheres e em pacientes infectados com genótipos não-1.BACKGROUND: Chronic infection by hepatitis C virus is an important public health issue. AIMS: To assess the effectiveness of a combination therapy with interferon-alpha plus ribavirin and to identify predictive factors of response in adult patients with chronic hepatitis C in a public drug supply program. PATIENTS AND METHODS: A retrospective study of consecutive records of 400 patients with chronic hepatitis C treated with interferon-a plus ribavirin therapy, as part of a health program conducted by the Department of Health of the State of Rio Grande do Sul, Porto Alegre, RS, southern Brazil, between 1999 and 2000, was carried out. RESULTS: Sex distribution was

  1. Análisis de consistencia interna mediante Alfa de Cronbach: un programa basado en gráficos dinámicos Internal consistency analysis by means of Cronbach’s Alpha: a computer program based on dynamic graphics

    OpenAIRE

    Rubén Ledesma; Gabriel Molina Ibañez; Pedro Valero Mora

    2002-01-01

    En este trabajo se presenta una herramienta informática original que permite realizar análisis de consistencia interna (modelo Alfa de Cronbach) utilizando métodos gráficos dinámicos. Se trata de un módulo basado en la filosofía del Análisis Exploratorio de Datos y en métodos de visualización estadística, diseñado para asistir al analista en el proceso de construcción de pruebas psicológicas. La herramienta permite analizar la consistencia interna de la prueba, las propiedades de los ítems qu...

  2. MODIFICACIÓN FÍSICA DEL ALMIDÓN DE YUCA Y EVALUACIÓN DE LA SUSCEPTIBILIDAD A LA HIDRÓLISIS ENZIMÁTICA POR UNA ALFA AMILASA

    OpenAIRE

    Jenny Martín; Elizabeth López

    2010-01-01

    Almidón de yuca comercial (variedad MTAI 8) se sometió a modificación física por sinéresis, extrusión, gelatinización y secado por rodillos. Al almidón nativo y a los almidones modificados se les determinó su morfología, cristalinidad, distribución molecular y susceptibilidad a la hidrólisis enzimática con una alfa amilasa porcina pancreática. En los almidones modificados físicamente se incrementó el grado de hidrólisis, en comparación con el almidón nativo. Sin embargo no se observaron difer...

  3. Determinação das constantes K e alfa da equação de Mark-Houwink de poli(p-acetóxiestireno Determination of K and alpha constants of the Mark-Houwink equation of poly(p-acetoxystyrene

    Directory of Open Access Journals (Sweden)

    José C. Moreira

    2004-06-01

    Full Text Available O conceito de volume hidrodinâmico pode ser utilizado efetivamente com cromatografia de permeação em gel (GPC e dados de viscosidade para calcular a massa molar de uma variedade de polímeros. Neste trabalho, as constantes da equação de Mark-Houwink para os homopolímeros de poli(p-acetóxiestireno (PASt foram determinadas pela correlação dos valores de massa molar ponderal média ( determinados por cromatografia de permeação em gel (GPC relativos a padrões de poliestireno, com as correspondentes medidas de viscosidade intrínseca [] em soluções de THF a 25 °C. A massa molar viscosimétrica média ( de qualquer amostra de PASt desconhecida pode ser calculada medindo-se a viscosidade intrínseca da amostra, e aplicando-se então a equação de Mark-Houwink, utilizando-se os valores de K e alfa calculados, 1,442 x 10-2 g/ml e 0,695, respectivamente.The hydrodynamic volume concept can be used effectively with gel-permeation chromatography (GPC and viscosity data to estimate the molecular weight of a variety of polymers. In this work, the Mark-Houwink coefficients (alpha and K for the poly(p-acetoxystyrene homopolymers (PASt were determined by correlating their weight average molecular weight ( values, determined by gel-permeation chromatography (GPC relative to polystyrene standards, with their measured intrinsic viscosity values ([]. The of any unknown PASt sample may be estimated by first measuring the sample's intrinsic viscosity, and then applying these alpha and K values with the Mark-Houwink equation.

  4. Daily interferon induction regimen using different manufactured interferons (alpha-2a or alpha-2b in combination with ribavirin for treatment of chronic hepatitis C: a prospective randomized study Esquema de indução diário com interferon alfa 2-a ou alfa 2-b em combinação com ribavirina para tratamento da hepatite crônica C: estudo prospectivo randomizado

    Directory of Open Access Journals (Sweden)

    Eduardo Lorens Braga

    2006-12-01

    .6% (50% IFN TIW vs 63.6% IFN QD CONCLUSIONS: Combination therapy had an overall sustained virological response rate of 39.8% ("intention to treat analysis". There was no difference with respect to sustained virological response rates between patients who used daily induction schedule compared to standard regimen. Adverse events, even more frequent in the daily induction group, did not interfere with the treatment strategies.RACIONAL: Estudos em cinética viral na hepatite C demonstraram que há uma queda dos níveis séricos de interferon 48 h após a sua administração, quando a carga viral do vírus C volta a se elevar. Antes da disponibilidade do interferon peguilado, diversos ensaios clínicos investigaram a terapia de indução com interferon standard OBJETIVOS: Avaliar a segurança e eficácia do esquema de indução diário com interferon alfa associado à ribavirina. PACIENTES E MÉTODOS: Noventa e três pacientes com hepatite crônica C foram incluídos. Através de randomização, foram alocados em um de dois braços terapêuticos: 44 indivíduos no grupo A: IFN 3MU três vezes por semana + ribavirina 1,0-1,2 g diariamente por 48 semanas e 49 indivíduos no grupo B: IFN 3MU diariamente por 12 semanas, seguindo-se por IFN 3MU três vezes por semana até completar 48 semanas + ribavirina 1,0-1,2 g diariamente por 48 semanas. A genotipagem do vírus C foi realizada em 85 indivíduos. Considerou-se resposta virológica sustentada a persistência do HCV-RNA negativo 6 meses após o término da terapia RESULTADOS: Oitenta e três pacientes completaram o tratamento. Houve cinco abandonos (um do grupo A e quatro do grupo B e em cinco pacientes a terapia foi retirada devido a efeitos adversos (dois do grupo A e três do grupo B. Não houve diferença estatisticamente significante entre os grupos quanto à interrupção do tratamento. A freqüência de cirrose foi 29%, semelhante entre os grupos. Na análise "intention to treat" a resposta virológica sustentada foi 39

  5. The frequency of β-globin gene haplotypes, α-thalassemia and genetic polymorphisms of methylenetetrahydrofolate reductase, factor V Leiden and prothrombin genes in children with sickle cell disease in Rio de Janeiro, Brazil Frequência dos haplótipos da globina beta, da talassemia alfa e dos polimorfismos genéticos dos genes da metilenotetrahidrofolato redutase, do fator V Leiden e da protrombina em crianças com doença falciforme no Rio de Janeiro, Brasil

    OpenAIRE

    Isaac L. Silva Filho; Ribeiro, Georgina S.; Leíse M. Pimenta-Bueno; Maria José A. Serpa

    2010-01-01

    A freqüência dos haplótipos beta S e beta C do gene da globina e a prevalência de talassemia alfa e de mutações nos genes da metilenotetrahidrofolato redutase (MTHFR-C677T), do fator V de Leiden e da protrombina (G20210A) foi estudada em crianças com doença falciforme do Rio de Janeiro. O haplótipo Bantu foi o mais freqüente (65,9%), 21,2% das crianças (18% heterozigotas e 3% homozigotas) apresentam talassemia com mutação alfa 3.7kb, ao contrário da mutação alfa 4.2kb que não foi encontrada. ...

  6. Doença de Ménétrier: relato de caso em menina Ménétrier's disease: a female child case report

    Directory of Open Access Journals (Sweden)

    Lidiana Morais Longo

    2007-09-01

    Full Text Available OBJETIVO: Relatar um caso de menina com doença de Ménétrier. DESCRIÇÃO DO CASO: Menina de dez anos, admitida com história de vômitos, diarréia, distensão e dor abdominal intermitentes há três dias. A suspeita foi apendicite e a paciente foi submetida à laparotomia, com achado de ascite e adenomegalia mesentérica. No pós-operatório, evoluiu com edema generalizado, ascite e derrame pleural. Os exames complementares e a evolução clínica demonstraram tratar-se de doença de Ménétrier. COMENTÁRIOS: O quadro clínico e os exames solicitados (alfa-1-antitripsina fecal aumentada, mucosa gástrica tipo fúndica com moderado grau de hiperplasia do epitélio luminal e foveolar e seriografia com hipertrofia do pregueado mucoso gástrico confirmaram a doença de Ménétrier.OBJECTIVE: To report a case of Ménétrier's disease in a girl. CASE DESCRIPTION: A 10-year-old girl was admitted to the hospital with a 3-day history of vomiting, diarrhea, intermittent distension and abdominal pain. Appendicitis was suspected, and patient had a laparotomy which showed only ascites and mesenteric adenomegaly. Generalized edema, ascites and right pleural effusion evolved after the surgery. Menetrier’s disease was diagnosed by laboratorial exams and clinical evolution. COMMENTS: Clinical aspects and the increased fecal alpha-1-antitrypsin associated to gastric mucosa of fundic type with moderate level of hyperplasia of lumina and foveola epithelium and serioscopy with hypertrophy of the plica of gastric mucosa confirmed Ménétrier's disease.

  7. Discinesia ciliar primária Primary ciliary dyskinesia

    Directory of Open Access Journals (Sweden)

    JOSÉ WELLINGTON ALVES DOS SANTOS

    2001-09-01

    Full Text Available Discinesia ciliar primária é uma doença autossômica recessiva caracterizada pela história de infecções repetidas do trato respiratório superior e inferior, otite média, bronquite e rinossinusite, associada a situs inversus na metade dos casos. O diagnóstico é estabelecido pela análise ciliar ultra-estrutural de espécimes respiratórios, após a exclusão inicial de outras doenças, como fibrose cística, deficiência de alfa-1-antitripsina, imunodeficiências (IgG, neutrófilos e complemento e síndrome de Young. O propósito deste artigo é revisar os achados clínicos, o diagnóstico e o manejo da discinesia ciliar primária, incluindo um fluxograma diagnóstico.Primary ciliary dyskinesia is an autosomal recessive disorder characterized by a history of recurrent upper and lower respiratory tract infections with chronic otitis media, bronchitis and rhinosinusitis, associated with situs inversus in 50% of cases. The diagnosis is established by ciliary ultrastructural analysis of respiratory specimens, after ruling out some disorders as cystic fibrosis, alpha-1 anti-trypsin deficiency, immune deficiencies (IgG, neutrophils and complement and Young's syndrome. The purpose of this paper is to review the clinical features, diagnosis and management of primary ciliary dyskinesia, including a diagnostic algorithm.

  8. Eficácia terapêutica do interferon alfa em criança com hemangioma gigante craniofacial: relato de caso Therapeutical effectiveness of interferon alpha in a child with craniofacial giant hemangioma: case report

    Directory of Open Access Journals (Sweden)

    Nilson Lopes da Fonseca Junior

    2008-06-01

    Full Text Available O hemangioma é o tumor benigno mais freqüente da infância. O hemangioma capilar geralmente apresenta-se como uma mancha ou tumoração violácea bem delimitada. O diagnóstico destas lesões é clínico. O hemangioma gigante é rara e extensa variação do hemangioma capilar, que geralmente ocorre em recém-nascidos e lactentes. Várias são as modalidades terapêuticas, como a injeção intralesional de corticóide, laserterapia, injeção intralesional de soluções esclerosantes, corticoterapia sistêmica, cirurgia, radioterapia e embolização. Novas modalidades terapêuticas têm sido desenvolvidas, com o objetivo de se obter melhores resultados e possibilitar o tratamento de lesões de difícil acesso cirúrgico e refratárias às modalidades terapêuticas utilizadas rotineiramente. Os melhores resultados tem sido obtidos com o interferon alfa. Este é um caso de uma paciente com três meses de idade, que apresentava desde o nascimento, tumoração arroxeada e amolecida em pálpebra superior do olho direito, lesões cutâneas planas e arroxeadas em região temporal e parietal direita. Realizada tomografia computadorizada de crânio evidenciando processo expansivo orbitário vascularizado com extensão para fossa média, seio cavernoso e fossa posterior. O tratamento inicial foi a corticoterapia oral durante quarenta dias, com redução progressiva por quatro semanas. Com o quadro praticamente inalterado, foi iniciado o tratamento com interferon alfa, na dose de 3.000.000 U/m², subcutâneo, três vezes por semana. Após 9 meses de tratamento, observa-se apenas uma pequena lesão orbitária residual. Neste caso, o interferon alfa apresentou-se como boa opção no tratamento do hemangioma gigante craniofacial.Hemangiomas are the most commom benign tumors of infancy. Capillary hemangioma generally is presented as a spot or well-defined purple lesion. The diagnosis of these tumors is based on physical examination. Giant hemangioma is a rare

  9. HBsAg seroconversion after pegylated interferon alfa 2a rescue in a lamivudine-resistant patient with HBeAg-negative chronic hepatitis B and favourable IL28-B genotype.

    Science.gov (United States)

    Stanzione, Maria; Stornaiuolo, Gianfranca; Rizzo, Viviana; Pontarelli, Agostina; Gaeta, Giovanni Battista

    2016-06-01

    Hepatitis B virus (HBV) surface antigen (HBsAg) seroconversion to anti-HBs antibody is the best final objective for all available chronic hepatitis B (CHB) treatments. Unfortunately, this goal is rarely achieved with the currently applied therapeutic approaches. Here we describe the case of an anti-HBe-positive CHB patient who was successfully treated with a particular therapeutic schedule. The patient was initially treated with lamivudine (LAM) for nine years. Breakthrough was observed after eight years of LAM therapy. HBV-DNA was 3x10E4 IU/mL and LAM resistance mutations were present. Subcutaneous pegylated interferon (PEG-IFN) alfa 2a, 180 mcg/week, was added to LAM and after 4 weeks LAM was discontinued and PEG-IFN alone was continued up to week 52. HBV-DNA became undetectable at week 4 of therapy; serum HBsAg started to decline from week 4 and became undetectable at week 36, with the subsequent appearance of anti-HBs antibodies. IL28-B was genotyped at the polymorphic site rs12979860 and the CC allele was detected. Rescue therapy with Peg-IFN may be an option for selected patients with resistance to nucleos(t)ide analogues. PMID:27367326

  10. Long term follow-up and patterns of response of ALT in patients with chronic hepatitis NANB/C treated with recombinant interferon-alpha Seguimento tardio e padrões de resposta da ALT em pacientes com hepatite crônica NANB/C tratados com interferon-alfa

    Directory of Open Access Journals (Sweden)

    L.C. da Silva

    1995-06-01

    Full Text Available The response to interferon treatment in chronic hepatitis NANB/C has usually been classified as complete, partial or absent, according to the behavior of serum alanine aminotransferase (ALT. However, a more detailed observation of the enzymatic activity has shown that the patterns may be more complex. The aim of this study was to describe the long term follow-up and patterns of ALT response in patients with chronic hepatitis NANB/C treated with recombinant interferon-alpha. A follow-up of 6 months or more after interferon-a was achieved in 44 patients. We have classified the serum ALT responses into six patterns and the observed frequencies were as follows: I. Long term response = 9 (20.5%; II. Normalization followed by persistent relapse after IFN = 7 (15.9%; III. Normalization with transient relapse = 5 (11.9%; IV. Temporary normalization and relapse during IFN = 4 (9.1%; V. Partial response (more than 50% of ALT decrease = 7 (15.9%; VI. No response = 12 (27.3%. In conclusion, ALT patterns vary widely during and after IFN treatment and can be classified in at least 6 types.A resposta ao tratamento com interferon em pacientes com hepatite crônica NANB/C tem sido classificada como completa, parcial ou ausente, de acordo com o comportamento da alanino aminotransferase sérica (ALT. Entretanto, uma observação mais detalhada da atividade enzimática tem mostrado que os padrões podem ser mais complexos. O objetivo deste estudo foi descrever o seguimento tardio e os padrões de resposta da ALT em pacientes com hepatite crônica NANB/C tratados com interferon alfa recombinante. Classificamos os tipos de resposta da ALT em 6 padrões e as freqüências observadas foram:I. Resposta completa e persistente = 9 (20,5%; II. Resposta completa com recaída persistente após o IFN = 7 (15,9%; III. Resposta completa com recaída temporária = 5 (11,9%; IV. Normalização temporária e recaída durante o tratamento com IFN = 4 (9,1%; V. Resposta parcial

  11. To Observe the Clinical Curative Effect of Interferon Alfa-2b Injection for the Treatment of Hepatitis C%重组人干扰素α-2b注射液治疗丙型肝炎的临床疗效观察

    Institute of Scientific and Technical Information of China (English)

    刘晓丹

    2014-01-01

    目的:观察重组人干扰素α-2b注射液治疗丙型肝炎的临床疗效。方法将我院2012年1月至2013年1月期间收治的80例丙型肝炎患者随机分为观察组40例和对照组40例。对照组患者在常规治疗的基础上行阿德福韦酯治疗,观察者患者在常规治疗的基础上行重组人干扰素α-2b注射液治疗。观察2组患者治疗前后的肝功能指标变化情况,统计2组患者治疗期间及治疗后HCV-RNA的阴转率及不良反应发生率。结果治疗后,观察组患者肝功能指标TBIL、ALT、AST平均水平明显低于对照组,2组比较,差异具有统计学意义( P0.05)。且2组患者均无对治疗不能耐受而停药者。结论重组人干扰素α-2b注射液治疗丙型肝炎的临床疗效显著,且安全性较好,值得临床推广与应用。%Objective To observe the clinical curative effect of interferonα-2b injection for the treatment of hepatitis C. Methods 80 cases of hepatitis C patients in our hospital from 2012 January to 2013 January were randomly divided into the ob-servation group(40 cases)and control group with 40 cases. On the same basis treatment, the control were added Adefovir dipivox-il, the observer patients added α-2b injection. To observe the changes of liver function indexes in the 2 groups before and after treatment. Results The observation group patients liver function indexes of TBIL, ALT, AST mean levels were significantly lower than the control group(P<0. 05). After 12,24 weeks, HCV-RNA negative conversion rate of the observation group were higher than those in the control group(P<0. 05). Conclusion The clinical efficacy of interferon alfa-2b injection for the treatment of hepatitis C were significant and safety.

  12. 干扰素α-2b联合利巴韦林治疗慢性丙型肝炎患者疗效观察%A clinical observation of interferon alfa-2b plus ribavirin in treatment of patients with chronic hepati-tis C

    Institute of Scientific and Technical Information of China (English)

    田慧; 和振坤; 徐庆杰

    2013-01-01

    目的:观察普通干扰素联合利巴韦林治疗慢性丙型肝炎患者的临床疗效。方法87例慢性丙型肝炎患者被随机分成对照组44例和观察组43例,均应用重组人干扰素α-2b 联合利巴韦林治疗,分别治疗48周和72周,随访24周,观察疗效。结果在44例对照组患者中,HCV 1b型感染者28例(63.6%),2a型感染者8例(18.2%),在观察组43例患者中则分别为29例(67.4%)和6例(14.0%);对照组获得快速病毒学应答率(RVR)、早期病毒学应答(EVR)、治疗结束时应答(ETR)和持续病毒学应答率(SVR)分别为40.9%、59.1%、68.2%和38.6%,而观察组则分别为39.5%、60.5%、88.4%(P<0.05)和65.1%(P<0.05);根据血清HCV RNA水平是否≥1×106copies/m1,将两组患者分为高病毒载量组和低病毒载量组,结果两组低病毒载量组患者的ETR和SVR均显著高于高病毒载量组(P<0.05)。结论延长干扰素联合利巴韦林治疗慢性丙型肝炎的疗程有助于提高SVR。%Objective To observe the clinical efficacy of interferon alfa-2b and ribavirin in the treatment of patients with chronic hepatitis C. Methods 87 patients with chronic hepatitis C were randomly divided into two groups,and the patients in both groups were treated with interferon alfa-2b and ribavirin. The regimen lasted for 48 weeks in 44 patients in control and for 72 weeks in 43 patients in the observation group. All the patients were followed-up for 24 weeks after discontinuation of the treatment. Results The HCV genotype 1b and 2a infections were found in 28(63.6%)and 8 patients(18.2%)in the control group,while they were in 29(67.4%)and 6 patients(14.0%)in the observation groups;The rapid virologic response(RVR),early virologic response(EVR), virologic response at the end of treatment(ETR)and sustained virologic response(SVR)in control group were 40.9%, 59.1%,68.2% and 38.6%,respectively,while they were 39.5%,60.5%,88.4%(P<0.05)and

  13. Novel Platinum-Catalyzed Ring-Opening of 1,2-Cyclopropanated Sugars with Alcohols

    DEFF Research Database (Denmark)

    Beyer, Jürgen; Madsen, Robert

    1998-01-01

    Reaction of 1,2-cyclopropanated sugars with a catalytic amount ofZeise's dimer [Pt(C2H4)Cl2]2 and an alcohol gives 2-C-branched glycosides by a novel platinum catalyzed ring-opening. A wide variety of alcohols can participate in this ring-opening reaction giving 2-C-branched glycosides ranging from...... methyl glycosides to complex disaccharides (17 examples). Very high diastereoselectivity is obtained at the newly formed C-1 stereocenter strongly favoring the alfa-glycoside. The mechanism presumably involves the formation of an intermediate platinacyclobutane which undergoes ring-opening at C-1 by the...

  14. Quantificação de ácido alfa-linolênico em caules e folhas de linho (Linum usitatissimum L. colhidos em diferentes estágios de desenvolvimento Quantification of alpha-linolenic acid in stems and leaves of flax (Linum usitatissimum L. harvested in different stages of development

    Directory of Open Access Journals (Sweden)

    Ana Carolina de Aguiar

    2010-12-01

    Full Text Available O objetivo deste estudo foi quantificar o ácido alfa-linolênico [LNA, 18:3 (n-3], avaliar a composição centesimal das folhas e caules de linho (Linum usitatissimum L. submetidos à secagem e colhidos em diferentes estágios de desenvolvimento (40, 80 e 120 dias, e determinar o potencial antioxidante das folhas colhidas aos 80 dias através do teste com o radical DPPH. As folhas obtiveram maiores teores de cinzas, proteína e lipídios totais em relação aos caules. Tanto as folhas quanto os caules apresentaram razões de AGPI/AGS e n-6/n-3 dentro dos valores considerados adequados para a alimentação. Os caules colhidos nos diferentes tempos não apresentaram diferenças significativas (PThe objective of this study was to quantify the alpha-linolenic acid [LNA, 18:3 (n-3] and to evaluate the proximate composition of leaves and stems of flax (Linum usitatissimum L. dried and harvested at different stages of development (40, 80 and 120 days, and to determine the antioxidant potential of the leaf harvested at 80 days using the test of DPPH radical. The leaves had higher levels of ash, protein and total lipids when compared to the stems. Both the leaves as the stems had ratios of PUFA/SFA and n-6/n-3 within the values considered suitable for food. Stems in the different stages showed no significant difference (P<0.05 of LNA content. Leaves harvested at 80 days showed the highest concentration of LNA, corresponding to 1,262.36 mg/100g dried leaf. The different extracts (methanol, butanol, acetate and water were efficient in the inhibition of DPPH radical, with emphasis on the butanolic and acetate fractions and the values of IC50 were approximately 42 ppm. These results highlight the nutritional potential and antioxidant activity of leaves and stems of flaxseed for future use in the animal and human feeding.

  15. Funciones del gen relacionado a la latencia (gen LR) del herpes virus bovino tipo 1 (BoHV-1) y otras proteínas expresadas durante la latencia

    OpenAIRE

    S.E. Pérez

    2009-01-01

    El herpesvirus bovino tipo 1 (BoHV-1) causa diversos síndromes clínicos en el ganado. Al igual que otros alfa-herpesvirus, el ciclo de vida del BoHV-1 puede dividirse en tres etapas: infección aguda, latencia y reactivación. Durante la infección aguda, BoHV-1 expresa una cascada de genes altamente regulados. Sin embargo, durante la latencia, la expresión de genes virales se ve restringida al gen relacionado a la latencia (gen LR) y al ORF-E. El gen LR es poliadenilado y sufre empalmes alterna...

  16. MODIFICACIÓN FÍSICA DEL ALMIDÓN DE YUCA Y EVALUACIÓN DE LA SUSCEPTIBILIDAD A LA HIDRÓLISIS ENZIMÁTICA POR UNA ALFA AMILASA

    Directory of Open Access Journals (Sweden)

    Jenny Martín

    2010-04-01

    Full Text Available Almidón de yuca comercial (variedad MTAI 8 se sometió a modificación física por sinéresis, extrusión, gelatinización y secado por rodillos. Al almidón nativo y a los almidones modificados se les determinó su morfología, cristalinidad, distribución molecular y susceptibilidad a la hidrólisis enzimática con una alfa amilasa porcina pancreática. En los almidones modificados físicamente se incrementó el grado de hidrólisis, en comparación con el almidón nativo. Sin embargo no se observaron diferencias estadísticamente significativas en el grado de hidrólisis entre los almidonesmodificados. El patrón de difracción de rayos X tipo A, presentado por el almidón de yuca nativo y su propiedad birrefringente se alteraron por los pretratamientos, presentándose difractogramas de estructuras amorfas y pérdida de la cruz de malta en los almidones modificados. La microscopía electrónica de barrido (MEB demostró alteración en la apariencia y estructura del gránulo nativo, dando lugar a partículas de formas irregulares con superficies fragmentadas y rugosas como resultado del proceso de modificación. La cromatografía de exclusión sobre sepharosa 6B confirmó la desaparición de la fracción de alto peso molecular presente en el almidón nativo, con el consecuente incremento de las fracciones de bajo peso molecular en los almidones modificados.

  17. Biomarcadores del estado inflamatorio: nexo de unión con la obesidad y complicaciones asociadas Inflammatory biomarkers: the link between obesity and associated pathologies

    Directory of Open Access Journals (Sweden)

    M.ª A. Zulet

    2007-10-01

    Full Text Available El objetivo de este trabajo ha consistido en realizar una revisión de los biomarcadores que actualmente se proponen como el nexo de unión entre la inflamación, la obesidad y complicaciones asociadas, seleccionando los estudios llevados a cabo y las cuestiones pendientes. Cada vez hay mayor evidencia científica de que la inflamación puede jugar un papel importante en la etiología de diversas enfermedades crónicas de gran relevancia para la salud pública. En los últimos años, distintos estudios han sugerido que la obesidad podría ser un desorden inflamatorio. Asimismo, el estrés oxidativo se ha propuesto como un potencial inductor de la inflamación y de la susceptibilidad a la obesidad y patología asociadas. Entre los biomarcadores relacionados con la obesidad, la resistencia insulínica, las enfermedades cardiovasculares y el síndrome metabólico se encuentran: el factor de necrosis tumoral alfa, interleuquinas 6 y 18, angiotensinógeno, factor de crecimiento TGF-beta, inhibidor de la activación del plasminógeno, leptina, resistina, proteína C reactiva, amiloide A, ácido siálico, marcadores de disfunción endotelial (factor von Willebrand, ICAMs, vCAMs factor 3 del sistema del complemento, haptoglobina, glicoproteína zinc-alfa2, eotaxina, visfatina, apelina, alfa1-antitripsina, vaspina, omentina, proteína transportadora de retinol 4, ceruloplasmina, adiponectina y desnutrina. Algunos de estos biomarcadores son buenos predictores de riesgo cardiovascular (inhibidor de la activación de plasminógeno 1, angiotensinógeno, fibrinógeno, ácido siálico, factor 3 del complemento y proteína C reactiva, adiposidad (leptina, visfatina, resistina, haptoglobina y/o resistencia insulínica (ácido siálico, proteína C reactiva, inhibidor de la activación de plasminógeno 1, factor von Willebrand. Sin embargo, todavía queda por dilucidar el papel de muchos de ellos en la etiología de la obesidad y comorbilidades asociadas en

  18. Ca(OH2 action on TNF-alpha and NO release in macrophage culture stimulated by Pseudomonas aeruginosa LPS = Ação do Ca(OH2 sobre a produção de TNF-alfa e NO de cultura de macrófagos estimulada por LPS de Pseudomonas aeruginosa

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    Queiroz, Celso Emanoel de Souza

    2008-01-01

    Full Text Available Objetivo: A ação do hidróxido de cálcio [Ca(OH2] com o sistema imune e o mecanismo de neutralização das bactérias e seus subprodutos ainda não foi completamente esclarecida. Neste estudo foi avaliada a capacidade do Ca(OH2 em neutralizar o lipopolissacarídeo (LPS de Pseudomonas aeruginosa, utilizando-se duas metodologias: liberação de Óxido Nítrico (NO e Fator de Necrose Tumoral Alfa (TNF-alfa em cultura de macrófagos peritoneais de camundongos. Metodologia: No ensaio do NO, as células peritoneais foram expostas a uma solução de LPS (25mg/mL e 50mg/mL; e à suspensão de LPS/Ca(OH2 em duas concentrações (50mg/ 25mg e 25mg/25mg. Após 8 horas de incubação, foi utilizado reagente de Griess, e a liberação de NO foi quantificada. No ensaio do TNF-alfa, a solução de LPS foi usada na concentração de 25mg/mL e o LPS/Ca(OH2 a 25mg/25mg. Após 24 horas, as células foram fixadas e coradas com cristal violeta, e os valores de absorbância foram obtidos. Os resultados foram expressos em micromols. Todos os testes foram realizados em triplicata. Resultados: A presença de Ca(OH2 nas duas concentrações avaliadas reduziu significativamente a liberação de NO e TNF-alfa. Conclusão: Pode-se concluir que o LPS bacteriano representa um forte estímulo para liberação destas citocinas, mas o hidróxido de cálcio foi capaz de neutralizar este efeito.

  19. A genetic validation study reveals a role of vitamin D metabolism in the response to interferon-alfa-based therapy of chronic hepatitis C.

    Directory of Open Access Journals (Sweden)

    Christian M Lange

    Full Text Available BACKGROUND: To perform a comprehensive study on the relationship between vitamin D metabolism and the response to interferon-α-based therapy of chronic hepatitis C. METHODOLOGY/PRINCIPAL FINDINGS: Associations between a functionally relevant polymorphism in the gene encoding the vitamin D 1α-hydroxylase (CYP27B1-1260 rs10877012 and the response to treatment with pegylated interferon-α (PEG-IFN-α and ribavirin were determined in 701 patients with chronic hepatitis C. In addition, associations between serum concentrations of 25-hydroxyvitamin D(3 (25[OH]D(3 and treatment outcome were analysed. CYP27B1-1260 rs10877012 was found to be an independent predictor of sustained virologic response (SVR in patients with poor-response IL28B genotypes (15% difference in SVR for rs10877012 genotype AA vs. CC, p = 0.02, OR = 1.52, 95% CI = 1.061-2.188, but not in patients with favourable IL28B genotype. Patients with chronic hepatitis C showed a high prevalence of vitamin D insufficiency (25[OH]D(3<20 ng/mL during all seasons, but 25(OHD(3 serum levels were not associated with treatment outcome. CONCLUSIONS/SIGNIFICANCE: Our study suggests a role of bioactive vitamin D (1,25[OH](2D(3, calcitriol in the response to treatment of chronic hepatitis C. However, serum concentration of the calcitriol precursor 25(OHD(3 is not a suitable predictor of treatment outcome.

  20. Double blind randomized clinical trial controlled by placebo with an alpha linoleic acid and prebiotic enriched cookie on risk cardiovascular factor in obese patients Ensayo clínico aletorizado doble ciego controlado con placebo con una galleta enriquecida en ácido alfa linoleico y prebióticos en el patrón de riesgo cardiovascular de pacientes obesos

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    D. A. de Luis

    2011-08-01

    Full Text Available Introduction: Inulin and FOS are prebiotics with potential benefit in cardiovascular risk factors. Alpha linolenic acid (ALA is the metabolic precursor of the long chain n-3 fatty acid eicosapentaenoic acid (20: 5n-3, this fatty acid has anti-inflammatory properties. The aim of our study was to evaluate the response of the cardiovascular risk profile in obese patients after inclusion in the diet of an ALA, FOS and inulin enriched-cookie. Material and methods: 36 patients were randomized in both branches: group I (inulin, FOS and ALA enriched cookie Gullon SL® and group II (control cookie. Previous and after 1 month of the treatment, a nutritional and biochemical study was realized. Results: 15 patients finished the procotol in each group. In group I, a significantly increase in soluble fiber (2.3 ± 0.8 g/day vs 7.7 ± 0.8 g/day: p Introducción: La inulina y los FOS son prebióticos con potenciales efectos beneficiosos a nivel cardiovascular. El acido alfa linolénico (ALA es el precursor del ácido eicosapentaenoico (20: 5n-3, presentando propiedades antinflamatorias. El objetivo de nuestro trabajo es evaluar la respuesta del perfil de riesgo cardiovascular en pacientes obesos tras la inclusión en la dieta de una galleta enriquecida en inulina, FOS y ALA. Material y métodos: Un total de 36 pacientes fueron randomizados a una de las siguientes ramas: galleta I (enriquecida con inulina, FOS y ALA y galleta II (galleta control (Gullón SL. Cada paciente recibió un total de 2 galletas al día (70 gramos de producto. Antes de iniciar el tratamiento y al mes, se realizó una valoración nutricional y analítica. Resultados: Finalizaron el protocolo un total de 15 pacientes en cada grupo. En el grupo I se produjo un aumento significativo en la ingesta de fibra soluble (2,3 ± 0,8 g/día vs 7,7 ± 0,8 g/día: p < 0,05 (inulina y FOS, así como ALA (0,6 ± 0,5 g/día vs 3,8 ± 0,5 g/día; p < 0,05. Se detectó en los pacientes varones que

  1. An open-label clinical trial of agalsidase alfa enzyme replacement therapy in children with Fabry disease who are naïve to enzyme replacement therapy

    OpenAIRE

    Goker-Alpan, Ozlem

    2016-01-01

    Ozlem Goker-Alpan,1 Nicola Longo,2 Marie McDonald,3 Suma P Shankar,4,5 Raphael Schiffmann,6 Peter Chang,7 Yinghua Shen,7 Arian Pano7 1Lysosomal Disorders Unit, Fairfax, VA, 2University of Utah, Salt Lake City, UT, 3Department of Pediatrics, Duke University, Durham, NC, 4Department of Ophthalmology, 5Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, 6Institute of Metabolic Disease, Baylor Research Institute, Dallas, TX, 7Shire, Lexington, MA, USA Background: Fol...

  2. An open-label clinical trial of agalsidase alfa enzyme replacement therapy in children with Fabry disease who are naïve to enzyme replacement therapy

    OpenAIRE

    Goker-Alpan O; Longo N; McDonald M; Shankar SP; Schiffmann R; Chang P; Shen Y; Pano A

    2016-01-01

    Ozlem Goker-Alpan,1 Nicola Longo,2 Marie McDonald,3 Suma P Shankar,4,5 Raphael Schiffmann,6 Peter Chang,7 Yinghua Shen,7 Arian Pano7 1Lysosomal Disorders Unit, Fairfax, VA, 2University of Utah, Salt Lake City, UT, 3Department of Pediatrics, Duke University, Durham, NC, 4Department of Ophthalmology, 5Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, 6Institute of Metabolic Disease, Baylor Research Institute, Dallas, TX, 7Shire, Lexington, MA, USA Background: Fol...

  3. Deppresion in the Level of Cadherin and alfa, á-, ç-catenins in Transgenic Xenopus laevis Highly Expressing c-Src

    Czech Academy of Sciences Publication Activity Database

    Dvořáková, K.; Habrová, V.; Takáč, Mirko; Jonák, Jiří

    2000-01-01

    Roč. 46, č. 1 (2000), s. 3-10. ISSN 0015-5500 R&D Projects: GA ČR GV312/96/K205 Institutional research plan: CEZ:AV0Z5052915 Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.351, year: 2000

  4. Synthesis of 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-mannopyranoside and 4-nitrophenyl 2-acetamido-2-deoxy-alfa-D-mannopyranoside

    Czech Academy of Sciences Publication Activity Database

    Krist, Pavel; Kuzma, Marek; Pelyvás, I. F.; Simerská, Pavla; Křen, Vladimír

    2003-01-01

    Roč. 68, - (2003), s. 801-811. ISSN 0010-0765 R&D Projects: GA ČR GA203/01/1018; GA MŠk ME 371 Institutional research plan: CEZ:AV0Z5020903 Keywords : staphylococcus autrus * bacillus subtilis Subject RIV: EE - Microbiology, Virology Impact factor: 1.041, year: 2003

  5. Pegylated interferon alfa and ribavirin for 14 versus 24 weeks in patients with hepatitis C virus genotype 2 or 3 and rapid virological response

    DEFF Research Database (Denmark)

    Dalgard, Olav; Bjøro, Kristian; Larsen, Helmer Ring;

    2008-01-01

    treatment-naïve HCV RNA-positive patients with genotype 2 or 3 were enrolled. Patients with RVR were randomized to 14 (group A) or 24 (group B) weeks of treatment. Patients were treated with pegylated interferon alpha-2b (1.5 microg/kg) subcutaneously weekly and ribavirin (800-1400 mg) orally daily...

  6. Deoxyiminoalditols from Aldonic Acids - VI. - Preparation of the Four Stereoisomeric 4-Amino-3-hydroxypyrrolidines from Bromodeoxytetronic Acids. Discovery of a New alfa-Mannosidase Inhibitor

    DEFF Research Database (Denmark)

    Lundt, Inge; Limberg, Gerrit; Zavilla, John

    ammonia. Thus, opposite regioselectivity for opening of the cis-configurated epoxide 3 (4 : 1, C-2 : C-3) and the trans-configurated epoxide 9 (3 : 7, C-2 : C-3) by ammonia was observed. - Initial testing as glycosidase inhibitors of the amino hydroxy pyrrolidines formed by reduction of the lactams showed...

  7. Estudio de la Crystalización no Isotérmica de Poli (Alfa-n-Alquil Beta-L-Aspartato)S Por DSC

    Czech Academy of Sciences Publication Activity Database

    Calventus, Y.; Colomer, P.; Málek, Jiří; Montserrat, S.; Lopéz-Carrasquero, F.; Martínez de Ilarduya, A.; Munoz-Guerra, S.

    Terrassa: CCP Centre Catalá del Plástic, 1997, s. 107-110. [Congresr International de Materiales Polimericos. V Reunión del Gep /1./. Terrassa (ES), 08.09.1997-10.09.1997] Grant ostatní: DGICYT(CZ) PB9 3/1241

  8. Exekutivní funkce a alfa a beta event-related de/synchronizace v subthalamickém jádře

    Czech Academy of Sciences Publication Activity Database

    Bočková, M.; Chládek, Jan; Jurák, Pavel; Halámek, Josef; Rektor, I.

    Brno : LF MU, 2007. s. 18. ISBN 978-80-254-0420-1. [Společný sjezd České a Slovenské společnosti klinické neurofyziologie /54./. 25.10.2007-26.10.2007, Brno] Institutional research plan: CEZ:AV0Z20650511 Keywords : subthalamic nucleus * SEEG * executive functions * ERD/S Subject RIV: FH - Neurology

  9. Treatment with pegylated interferon alpha 2b and ribavirin in patients unresponsive to previous treatments with standard interferon as monotherapy or combined with ribavirin Tratamiento con PEG interferón alfa 2b y ribavirina en enfermos previamente tratados con interferón estándar en monoterapia o combinado con ribavirina

    Directory of Open Access Journals (Sweden)

    F. Carnicer

    2005-05-01

    Full Text Available Background: little information is available on the effect of pegylated interferon (PEG and ribavirin (RBV in patients with chronic hepatitis due to virus C (CHC who were non-responders to previous treatment. Objectives: to evaluate response to treatment in patients who were non-responders to previous treatment. Methods: one hundred and twenty-four patients who were non-responders to previous treatment were included. All patients were treated with PEG alpha 2b interferon (dose: 1.5 mg/kg body weight and RBV (weight-dependent dosage. A qualitative PCR of virus C after six months was evaluated. In those in whom this was positive, treatment was discontinued; in those who were negative treatment was continued to the end of the year. Results: response following treatment (RFT was 35.4% (44 patients, and sustained viral response (SVR 29.8% (37 patients. No relation was observed between RFT, SVR and any previous treatment. RFT was dependent on low initial viremia and SVR was significantly and independently related to low serum hepatitis C RNA and a non-1 genotype. In general, treatment was well tolerated. Medication was discontinued in 5 patients, and doses reduced in 18. Conclusion: on retreatment with PEG and RBV a SVR of 29.8% was achieved in patients who had not responded to previous treatment, so its use in this group of patients is indicated.Introducción: existe poca información del resultado del tratamiento con interferón pegilado (PEG y ribavirina (RBV en enfermos con hepatitis crónica por virus C (HCC no respondedores a tratamientos previos. Objetivos: valorar la respuesta al tratamiento en enfermos no respondedores a tratamientos anteriores. Métodos: se incluyeron 124 enfermos no respondedores a tratamientos previos. Todos los pacientes fueron tratados con PEG interferón alfa 2b (dosis: 1,5 mg/kg peso y RBV (dosis ajustada al peso. Se valoró la PCR cualitativa del virus C al 6º mes. A los que la presentaban positiva se suspendió el

  10. Polimorfismos genéticos para Alfa Actinina 3 e sua relaçao com dano muscular e capacidades funcionais em atletas de futebol

    OpenAIRE

    Mendonça Pimenta, Eduardo

    2013-01-01

    La presente Tesis se compone de dos estudios independientes sobre comportamientos fisiológicos en atletas de fútbol con diferentes genotipos para el gene de la ACTN3. ESTUDIO 1 En este estudio investigamos el efecto de la ACTN3 o la ausencia de la ACTN3 en el desempeño físico de atletas de fútbol. Los autores evaluaron 200 atletas profesionales de fútbol en cuatro diferentes pruebas físicos que evalúan tanto a potência/força y resistencia. Fueron realizados dos variaciones de un sa...

  11. AMP-activated Protein Kinase alfa2 Subunit Is Required for the Preservation of Hepatic Insulin Sensitivity by n-3 Polyunsaturated Fatty Acids

    Czech Academy of Sciences Publication Activity Database

    Jeleník, Tomáš; Rossmeisl, Martin; Kuda, Ondřej; Macek Jílková, Zuzana; Medříková, Daša; Kůs, Vladimír; Hensler, Michal; Janovská, Petra; Mikšík, Ivan; Baranowski, M.; Gorski, J.; Hébrard, S.; Jensen, T. E.; Flachs, Pavel; Hawley, S.; Viollet, B.; Kopecký, Jan

    2010-01-01

    Roč. 59, č. 11 (2010), s. 2737-2746. ISSN 0012-1797 R&D Projects: GA ČR GAP301/10/1420; GA MŠk(CZ) 1M0520; GA MŠk(CZ) OC08007 Grant ostatní: EC(CZ) LSHM-CT-2004-005272 Institutional research plan: CEZ:AV0Z50110509 Keywords : AMPK * n-3 LC-PUFA * insulin resistance Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition Impact factor: 8.889, year: 2010

  12. Chiral analysis of alfa-diimine Ru(II) and Fe(II) complexes by capillary electrophoresis using sulfated cyclodextrins as stereoselectors

    Czech Academy of Sciences Publication Activity Database

    Sázelová, Petra; Koval, Dušan; Severa, Lukáš; Teplý, Filip; Kašička, Václav

    Olomouc: Palacký University, 2014 - (Maier, V.; Ševčík, J.), s. 198-199 ISBN 978-80-244-3950-1. ISSN 0232-0061. [Advances in Chromatography and Electrophoresis & Chiranal 2014. Olomouc (CZ), 10.02.2014-14.02.2014] R&D Projects: GA ČR(CZ) GAP206/12/0453; GA ČR(CZ) GA13-17224S; GA ČR GA13-32974S Institutional support: RVO:61388963 Keywords : capillary electrophoresis * chiral analysis * metal-polypyridyl complexes Subject RIV: CB - Analytical Chemistry, Separation

  13. Tolerability of intensified intravenous interferon alfa-2b versus the ECOG 1684 schedule as adjuvant therapy for stage III melanoma: a randomized phase III Italian Melanoma Inter-group trial (IMI – Mel.A.) [ISRCTN75125874

    International Nuclear Information System (INIS)

    High-dose interferon alfa-2b (IFNalfa-2b), according to the ECOG 1684 schedule, is the only approved adjuvant treatment for stage III melanoma patients by the FDA and EMEA. However, the risk/benefit profile has been questioned limiting its world-wide use. In the late nineties, the Italian Melanoma Inter-group started a spontaneous randomized clinical trial (RCT) to verify if a more intense, but shorter than the ECOG 1684 regimen, could improve survival without increasing the toxicity profile. The safety analysis in the first 169 patients who completed the treatment is here described. Stage III melanoma patients were randomized to receive IFNalfa-2b 20 MU/m2/d intravenously (IV) 5 days/week × 4 weeks, repeated for three times on weeks 9 to 12, 17 to 20, 25 to 28 (Dose-Dense/Dose-Intense, DD/DI, arm), or IFNalfa-2b 20 MU/m2/d IV 5 days/week × 4 weeks followed by 10 MU/m2 subcutaneously (SC) three times per week × 48 weeks (High Dose Interferon, HDI, arm). Toxicity was recorded and graded, according to the WHO criteria, as the worst grade that occurred during each cycle. The most common toxicities in both arms were flu-like and gastrointestinal symptoms, leukopenia, liver and neuro-psichiatric morbidities; with regard to severe toxicity, only leukopenia was statistically more frequent in DD/DI arm than in HDI arm (24% vs 9%) (p = 0.0074), yet, this did not cause an increase in the infection risk. Discontinuation of treatment, due to toxicity, was observed in 13 and 17% of the patients in the DD/DI and HDI arm, respectively. The median actual dose intensity delivered in the DD/DI arm (36.4 MU/m2/week) was statistically higher than that delivered in the HDI arm (30.7 MU/m2/week) (p = 0.003). Four cycles of intravenous high-dose IFNalfa-2b can be safely delivered with an increase in the median dose intensity. Efficacy results from this trial are eagerly awaited

  14. Code Betal to calculation Alpha/Beta activities in environmental samples; Programa de ordenador Betal para el calculo de la actividad Beta/Alfa de muestras ambientales

    Energy Technology Data Exchange (ETDEWEB)

    Romero, L.; Travesi, A.

    1983-07-01

    A codes, BETAL, was developed, written in FORTRAN IV, to automatize calculations and presentations of the result of the total alpha-beta activities measurements in environmental samples. This code performs the necessary calculations for transformation the activities measured in total counts, to pCi/1., bearing in mind the efficiency of the detector used and the other necessary parameters. Further more, it appraise the standard deviation of the result, and calculus the Lower limit of detection for each measurement. This code is written in iterative way by screen-operator dialogue, and asking the necessary data to perform the calculation of the activity in each case by a screen label. The code could be executed through any screen and keyboard terminal, (whose computer accepts Fortran IV) with a printer connected to the said computer. (Author) 5 refs.

  15. Masculinização de larvas de tilápia do Nilo (Oreochromis niloticus L. a partir de banhos de imersão com 17alfa-metiltestosterona Masculinization of larvae of Nile tilapia (Oreochromis niloticus L. by immersion baths with alpha-methyltestosterone

    Directory of Open Access Journals (Sweden)

    Robie Allan Bombardelli

    2005-04-01

    Full Text Available Determinaram-se os períodos ontogênicos de maior sensibilidade das larvas de tilápias do Nilo (Oreochromis niloticus à masculinização, com 17 alfa-metiltestosterona (MT, a partir de banhos de imersão, contendo 2,0 mg MT.L-1, por 36 horas, em um experimento realizado em duas fases - a primeira com tratamentos hormonais e a segunda na fase de alevinagem. Foram utilizadas 1.200 larvas provenientes de um mesmo lote de reprodutores e distribuídas em 24 recipientes plásticos (0,5 L, em delineamento experimental inteiramente casualizado, composto por seis tratamentos hormonais e quatro repetições. Considerou-se, como unidade experimental, um recipiente plástico contendo 0,5 L de solução hormonal e 50 larvas. Os tratamentos constituíram-se na imersão das larvas em diferentes fases de desenvolvimento ontogênico, correspondentes a 133,7 UTAs (dias - grau ou 5 DPE (dias após a eclosão; 188,2 UTAs ou 7 DPE; 242,3 UTAs ou 9 DPE; 296,8 UTAs ou 11 DPE; 352,6 UTAs ou 13 DPE; e 408,2 UTAs ou 15 DPE. Após os tratamentos, as larvas foram cultivadas até atingirem o comprimento padrão mínimo de 25 mm. Os resultados de masculinização evidenciaram a existência de um período de maior sensibilidade aos 15 DPE ou 408,2 UTAs, com 85,19% de machos. Observou-se relação linear positiva entre o período ontogênico e as taxas de masculinização. As médias de comprimento total e peso final, fator de condição e sobrevivência não diferiram entre os tratamentos.An experiment was carried out to in two steps (the first was hormonal treatments and the other the larvae grow up phase to determine the best ontogenic periods for larvae of the Nile tilapia (Oreochromis niloticus regarding masculinization hormonal treatments with 17 alpha-methyltestosterone (MT by immersion baths in 2.0 mg MT.L-1 solution for 36 hours. One thousand and two hundred larvae of Nile tilapia, from the same reproduction bunch, were allotted to 24 plastic containers with 0.5 L

  16. Extrahepatic manifestations of chronic hepatitis C and their influence on response to treatment with Pegylated interferon alfa-2a and ribavirin

    Directory of Open Access Journals (Sweden)

    Fabri Milotka

    2013-01-01

    Full Text Available Introduction. Thirty to 50% of patients with chronic hepatitis C (CHC have one or more extrahepatic manifestations (EHMs of hepatitis C virus (HCV infection. Objective. The aim of this study was to evaluate the frequency of EHMs and to investigate the efficacy of pegylated interferon (Peg­IFN­α­2a plus ribavirin therapy in patients with HCV­related EHMs. Methods. The study included 280 patients suffering from CHC and treated with Peg­IFN­α­2a and ribavirin. The patients were divided in two groups according to presence or absence of EHMs. We evaluated virological response to antiviral therapy. Results. One or more EHMs were found among 27.9% of patients. Most frequently they had rheumatoid factor in serum (12.5%, organ­nonspecific antibodies ANA and AGMA (12.4%, thyroid hormone disorders (9.3%, vasculitis (5.7%, diabetes mellitus (4.65%, glomerulonephritis (0.71%, and porphyria cutanea tarda (0.36%. Among the patients with EHMs there was 52.6% of females vs. 30.2% of females in the group of patients without EHMs (p=0.001. HCV genotypes 1 and 4 had 85.9% patients with EHMs vs. 58.4% of patients without EHMs (p=0.000. Progressive fibrosis and cirrhosis were more frequently recorded in the EHM group of patients (32% vs. 23.2%, but without statistically significant difference (p=0.532. Serious adverse events of Peg­IFN­α­2a and ribavirin were statistically significantly recorded among the patients with EHMs (46.1% vs. 12.9%; p=0.000. Sustained virological response among the patients with and without EHMs rated 56.9% and 70.8% respectively (p=0.125. Conclusion. Patients with CHC and EHMs treated with combined Peg­IFN­α­2a and ribavirin experience handling difficulties, more often have serious adverse events, while successful outcome is achieved in about 50% of patients.

  17. “DE ORNITORRINCOS À ALFA CENTAURI”: GOSTO PELO EXÓTICO E MULTICULTURALISMO NA ESTÉTICA DE JEAN-FRANÇOIS LYOTARD

    Directory of Open Access Journals (Sweden)

    Luciana Molina Queiroz

    2013-06-01

    Full Text Available Este artigo se propõe a discutir a estética de Jean-François Lyotard a partir de dois eixos principais: 1 sua concepção de cultura de massa e 2 sua concepção de arte. Mediante a interpretação de algumas das principais obras de Lyotard, objetivamos mostrar como, ao longo da produção teórica de Lyotard sobre o pós-moderno, encontra-se uma valorização do novo, do exótico e do multiculturalismo que coloca o filósofo em dificuldades para definir cultura de massa e arte. A hipótese que norteia este artigo é a de que Lyotard por vezes deliberadamente confunde os dois domínios, de modo que seu pensamento deixa de ser crítico em relação às produções culturais capitalistas, tornando-se conivente com o consumismo presente nas sociedades contemporâneas.

  18. Role of elosulfase alfa in mucopolysaccharidosis IVA

    OpenAIRE

    Regier, Debra

    2016-01-01

    Debra S Regier, Pranoot Tanpaiboon Division of Genetics and Metabolism, Children’s National Medical Center, Washington, DC, USA Abstract: Mucopolysaccharidosis type IVA (MPS IVA or Morquio A) is an autosomal recessive lysosomal storage disease which results in a striking skeletal phenotype, but does not negatively impact the intellect of the patient. MPS IVA has a phenotypic continuum that ranges from a severe and rapidly progressing form to a slowly progressive form. The c...

  19. Role of elosulfase alfa in mucopolysaccharidosis IVA

    OpenAIRE

    Regier DS; Tanpaiboon P

    2016-01-01

    Debra S Regier, Pranoot Tanpaiboon Division of Genetics and Metabolism, Children’s National Medical Center, Washington, DC, USA Abstract: Mucopolysaccharidosis type IVA (MPS IVA or Morquio A) is an autosomal recessive lysosomal storage disease which results in a striking skeletal phenotype, but does not negatively impact the intellect of the patient. MPS IVA has a phenotypic continuum that ranges from a severe and rapidly progressing form to a slowly progressive form. The clinical ...

  20. The ALFA Roman Pot Detectors of ATLAS

    CERN Document Server

    Khalek, S Abdel; Anghinolfi, F; Barrillon, P; Blanchot, G; Blin-Bondil, S; Braem, A; Chytka, L; Muíño, P Conde; Düren, M; Fassnacht, P; Franz, S; Gurriana, L; Grafström, P; Heller, M; Haguenauer, M; Hain, W; Hamal, P; Hiller, K; Iwanski, W; Jakobsen, S; Joram, C; Kötz, U; Korcyl, K; Kreutzfeldt, K; Lohse, T; Maio, A; Maneira, M J P; Notz, D; Nozka, L; Palma, A; Petschull, D; Pons, X; Puzo, P; Ravat, S; Schneider, T; Seabra, L; Sykora, T; Staszewski, R; Stenzel, H; Trzebinski, M; Valkar, S; Viti, M; Vorobel, V; Wemans, A

    2016-01-01

    The ATLAS Roman Pot system is designed to determine the total proton-proton cross-section as well as the luminosity at the Large Hadron Collider (LHC) by measuring elastic proton scattering at very small angles. The system is made of four Roman Pot stations, located in the LHC tunnel in a distance of about 240~m at both sides of the ATLAS interaction point. Each station is equipped with tracking detectors, inserted in Roman Pots which approach the LHC beams vertically. The tracking detectors consist of multi-layer scintillating fibre structures readout by Multi-Anode-Photo-Multipliers.

  1. Peginterferon Alfa-2b Injection (Sylatron)

    Science.gov (United States)

    ... You may experience flu-like symptoms such as fever, chills, muscle aches, joint pain, tiredness, and headache after ... include: extreme tiredness headache muscle pain sore throat, fever, chills, and other signs of infection unusual bruising or ...

  2. CONFIRMAÇÃO DA IDENTIDADE DA alfa-CRIPTOXANTINA E INCIDÊNCIA DE CAROTENÓIDES MINORITÁRIOS PROVITAMÍNICOS A EM VERDURAS FOLHOSAS VERDES CONFIRMATION OF THE IDENTITY OF alpha-CRYPTOXANTHIN AND INCIDENCE OF MINOR PROVITAMIN A CAROTENOIDS IN GREEN LEAFY VEGETABLES

    Directory of Open Access Journals (Sweden)

    Adriana Z. MERCADANTE

    2001-08-01

    Full Text Available Numerosos trabalhos comprovaram que os carotenóides principais de folhas verdes são invariavelmente luteína, beta-caroteno, violaxantina e neoxantina. No entanto, há discordância em torno dos carotenóides minoritários. Portanto, a espectrometria de massas por impacto de elétrons e cromatografia líquida de alta eficiência com detector de arranjo de diodos foram utilizados para confirmar a identidade de carotenóides minoritários com atividade provitamínica A em verduras folhosas brasileiras. Os carotenóides pró-vitamínicos A, incluindo os isômeros cis e trans de beta-caroteno, foram separados em coluna de C18 polimérica, Vydac 201TP54, com metanol/água (98:2 como fase móvel. Os espectros UV-visível e de massas confirmaram o carotenóide monoidroxilado como sendo alfa-criptoxantina e não beta-criptoxantina como aponta a literatura internacional. Todas as onze folhas analisadas (agrião, alface crespa, alface lisa, almeirão, caruru, chicória, couve, espinafre, rúcula, salsinha e taioba apresentaram alfa-criptoxantina, 13-cis-beta-caroteno e 9-cis-beta-caroteno, enquanto que alfa-caroteno foi encontrado em apenas quatro folhas (caruru, couve, salsinha e taioba.The main carotenoids from green leafy vegetables have been consistently found to be lutein, beta-carotene, violaxanthin and neoxanthin. However, there is a controversy about the identity of minor carotenoids. Therefore, electron impact mass spectrometry and high performance liquid chromatography with a diode array detector were used in order to confirm the identity of the minor provitamin A carotenoids in Brazilian green leaves. The provitamin A carotenoids, including the cis and trans isomers of beta-carotene, were separated on a polymeric C18 column, Vydac 201TP54, with MeOH/H2O (98:2 as mobile phase. The UV-visible and mass spectra confirmed that the monohydroxy carotenoid present in Brazilian green leafy vegetable to be alpha-cryptoxanthin, and not beta

  3. Structures of thymidine kinase 1 of human and mycoplasma origin

    DEFF Research Database (Denmark)

    Welin, Martin; Kosinska, Urszula; Mikkelsen, Nils-Egil;

    2004-01-01

    Cytosolic thymidine kinase, TK1, is a well-known cell cycle regulated enzyme of importance in nucleotide metabolism as well as an activator of antiviral and anticancer drugs as AZT. We have now determined the first structures of the TK1 family, the human and Ureaplasma urealyticum enzymes, in...... complex with the feedback inhibitor dTTP. The TK1s have a tetrameric structure where each subunit contains an alfa/beta-domain that is similar to ATPase domains of members of the RecA structural family and a domain of a new type containing a structural zinc. The zinc ion connects beta-structures at the...... differs fundamentally from the structures of the other deoxyribonucleoside kinases indicating a different evolutionary origin....

  4. Comparação entre a relação PCR/albumina e o índice prognóstico inflamatório nutricional (IPIN Comparison of PCR/albumin ratio with prognostic inflammatory nutritional index (PINI

    Directory of Open Access Journals (Sweden)

    Camila Renata Corrêa

    2002-07-01

    Full Text Available Objetivo: Simplificar o cálculo do índice prognóstico inflamatório nutricional (IPIN empregando número menor de variáveis com conseqüente redução do custo da análise. Materiais e métodos: Foram estudados 54 pacientes e 12 indivíduos-controle com 48 ± 20 (média ± dp anos de idade. As principais patologias dos pacientes eram: doença arterial periférica (22, pênfigo foliáceo (7, doença inflamatória intestinal (7, trauma (6 e pós-operatório de ortognatia (3. Foram obtidas amostras de sangue periférico, colhidas em jejum para dosagens de proteínas positivas (+ e negativas (- de fase aguda (PFA pelo método nefelométrico. Proteína C reativa (PCR, alfa-1-glicoproteína-ácida (alfa-1-GA, alfa-1-antitripsina (alfa-1-AT e ceruloplasmina (CER foram as PFA+ e albumina (Alb, transtiretina (TTR, transferrina (TF e proteína ligadora do retinol (RBP foram as representantes das PFA-. Esses valores foram analisados quanto à associação de correlação isolada ou associadamente na fórmula do índice prognóstico inflamatório e nutricional (IPIN = PCR + alfa-1-GA / Alb + TTR. De acordo com o índice prognóstico inflamatório e nutricional, os pacientes foram classificados em grupo-controle (G1; pacientes sem infecção/inflamação (IPIN 1, G3. Em seguida os pacientes do G3 foram subdivididos em baixo risco (G3A, n = 16; médio risco ( G3B, n = 10; alto risco (G3C, n = 6 e com risco de morte (G3D, n = 11. Os resultados foram correlacionados entre si (teste de Spearman ou submetidos às comparações entre grupos (teste de Kruskall-Wallis. Resultados: Houve relação significativa entre as variáveis PCR ´ alfa-1-GA (r = 0,49, Alb ´ TTR (r = 0,60, Alb ´ RBP (r = 0,58, Alb ´ TF (r = 0,39, TTR ´ RBP (r = 0,56 e TTR´ TF (r = 0,43 e as melhores relações encontradas entre PFA+ e PFA- foram: PCR ´ Alb (r = - 0,71, PCR ´ TTR (r = - 0,54, PCR ´ TF (r = - 0,39 e alfa-1-GA ´ Alb (r = - 0,35. Os valores do IPIN mostraram a

  5. Avaliação do desempenho organizacional da empresa familiar Indústrias Alfa S.A.* com base no modelo de gestão estratégica balanced scorecard Organizational performance of a family business evaluated by the strategic performance balanced scorecard

    Directory of Open Access Journals (Sweden)

    Christine Schneider

    2006-03-01

    Full Text Available O balanced scorecard é um moderno modelo estratégico de desempenho organizacional que visa traduzir a missão, visão e estratégia em objetivos e medidas, organizados sob a ótica financeira, dos clientes, dos processos internos, do aprendizado e do crescimento. O modelo disponibiliza estrutura e linguagem para comunicar a estratégia da empresa e o alcance das metas no longo prazo. A Indústrias Alfa S.A. é uma empresa familiar de segunda geração, que tem por visão estratégica "Ser a escolha preferencial de soluções hidráulicas da América Latina nos setores residencial, industrial, agrícola e predial", orientada pelo macroobjetivo de oferecer soluções e serviços em motobombas hidráulicas de modo a satisfazer as necessidades dos clientes. Partindo desses princípios, o artigo avalia o desempenho organizacional da empresa familiar Indústrias Alfa S.A. no primeiro quadrimestre do ano de 2005, com base no modelo de controle estratégico balanced scorecard.The balanced scorecard is a modern strategic model of organizational performance that seeks to translate the company mission, vision and strategy into objectives and measures organized to focus on - finance, customers, internal procedures, learning and growth. This model provides structure and language to communicate business strategy and directs efforts, abilities and specific knowledge to achieve long term objectives. The strategic vision of this second generation family business is "to be the answer for hydraulic pump needs in the Latin American residential, industrial, agricultural and building management markets" oriented by an overall objective of offering solutions and services to satisfy customer needs. Based upon these considerations, performance of this family business was evaluated during the first four months of 2005 using the strategic performance balanced scorecard.

  6. Thermal neutrons response of PS1 photographic dosimeters

    International Nuclear Information System (INIS)

    The PS1 photographic dosemeter is used by the dosimetry exploitation laboratory of IPSN (institute of protection and nuclear safety) for the regulatory medical surveillance of workers who have risks of exposure to ionizing radiations.It is an ALFA dosimetric film and a case (PS1) equipped with seven screens. The responses of Pb+Sn+Cd and Pb+Sn screens allow to measure the doses coming from high energy photons (higher than 150 keV) and thee one coming from thermal neutrons. This report describes and analyses the results of a recent series of measurements in the aim to check the answer of this dosemeter to thermal neutrons and to determine the transfer coefficients of visible doses in Co60, under the mentioned screens, in doses equivalents coming from thermal neutrons and photons. (N.C.)

  7. Efficacy of combination therapy with pegylated-interferon alfa-2a plus ribavirin in autoantibody-positive chronic hepatitis C patients%聚乙二醇干扰素α-2a联合利巴韦林治疗自身免疫抗体阳性慢性丙型肝炎的临床疗效

    Institute of Scientific and Technical Information of China (English)

    李雅馨; 杨彦佳; 杨梅; 陈立宇; 卢家桀; 马元吉; 刘凯; 雷学忠; 唐红

    2013-01-01

    目的 观察聚乙二醇干扰素α-2a (Peg-IFN α-2a)联合利巴韦林(RBV)治疗自身免疫抗体阳性慢性丙型肝炎患者的疗效,并与自身免疫抗体阴性的患者比较,探讨自身免疫抗体是否影响慢性丙型肝炎疗效. 方法 86例慢性丙型肝炎患者完善自身免疫抗体等检测后,按体质量给予Peg-IFN α-2a 135 μg或180 μg,每周1次,皮下注射;联合按体质量分配的利巴韦林800~ 1200mg/d,分次口服,共治疗48周.分别检测治疗前,治疗后4、12、24、36、48周以及治疗终止后24周的HCV RNA载量.数据比较用单因素方差分析或x2检验.结果 24例患者检出至少1种自身免疫抗体,其中抗线粒体抗体(ANA)阳性14例,抗平滑肌抗体阳性5例,抗1型肝肾微粒抗体阳性3例,ANA合并抗SSB抗体阳性1例,ANA合并抗肝细胞胞浆抗原1型抗体阳性1例.24例自身免疫抗体阳性患者中,17例(70.8%)获得了快速病毒学应答(RVR),19例(79.2%)获得了持续病毒学应答(SVR);62例自身免疫抗体阴性的患者中,45例(72.5%)获得了RVR,51例(82.2%)获得了SVR,两组患者的RVR及SVR率差异均无统计学意义(x2值分别为0.026和0.001,P值均> 0.05).结论 Peg-IFN α-2a联合RBV治疗自身免疫抗体阳性的慢性丙型肝炎患者仍然有显著疗效;自身免疫抗体的检出可能不是慢性丙型肝炎患者疗效的独立预测因素.%Objective To evaluate the therapeutic efficacy of antiviral combination therapy with pegylated-interferon alfa-2a(Peg-IFNα-2a) plus ribavirin (RBV) in patients with autoantibody-positive chronic hepatitis C (CHC) and to investigate the impact of the presence of autoantibodies on the treatment outcome.Methods Eighty-six consecutive CHC patients who underwent a 48-week treatment regimen composed of Peg-IFNα-2a (135 or 180 μg/wk) plus weight-based RBV (≤ 65 kg,800 mg/d; 65~75 kg,1000 mg/d; ≥ 75 kg,1200 mg/d).Prior to treatment (baseline) and at end of treatment (EOT; week 48

  8. 不同剂量干扰素α治疗慢性丙型肝炎疗效与安全性的系统评价%Effectiveness and Safety of Different Doses of Interferon Alfa in the Treatment of Chronic Hepatitis C: A Systematic Review

    Institute of Scientific and Technical Information of China (English)

    苏娜; 吴逢波; 徐珽

    2013-01-01

    Objective To evaluate the effectiveness and safety of different doses of interferon alfa (INF-α) in the treatment of chronic hepatitis C (CHC). Methods Such databases as MEDLINE, EMbase, CENTRAL, CBM, CNKI, VIP and WanFang Data were searched to collect the randomized controlled trials (RCTs) on different doses of INF-α in the treatment of CHC published before August, 2012. According to the inclusion and exclusion criteria, two reviewers independently screened literature, extracted data and evaluated the quality of the included studies, and then meta-analysis was performed using RevMan 5.0 software. Results A total of 13 RCTs involving 1 442 patients were included. The results of meta-analysis on different doses of INF-α showed that, a) There was no significant difference in the complete response rate between the 3 MU dose group and the 1 MU dose group (RR=0.83, 95%CI 0.52 to 1.32, P=0.43), but there was significant difference in the sustained response rate between those 2 groups (RR=1.89, 95%CI 1.00 to 3.59, P=0.05); and b) No significant differences were found in the complete response rate among the 3 MU dose group, the 6 MU dose group, and the 1 MU dose group. Conclusion INF-α in dose of 3 MU, 3 times daily, is effective in treating CHC, but it would not rule out that higher dose takes more effective action. When INF-α is used to treat CHC, an individualized medication should be applied according to patients' tolerance and economic status.%目的 系统评价不同剂量干扰素α治疗慢性丙型肝炎的疗效与安全性.方法 计算机检索MEDLINE、EMbase、CENTRAL、CBM、CNKI、VIP和WanFang Data,查找有关不同剂量干扰素α治疗慢性丙型肝炎的随机对照试验,检索时限截至2012年8月.由2位研究者根据纳入与排除标准独立筛选文献、提取资料并评价质量后,采用RevMan 5.0软件进行Meta分析.结果 最终纳入13个RCT,共1 442例患者.Meta分析结果显示:①干扰素α3MU组与1 MU组治疗慢性

  9. Vliv TGF-beta 1 na diferenciaci leukemických buněk HL-60 indukovanou společným působením TNF-alfa a inhibitory metabolismu kyseliny arachidonové

    Czech Academy of Sciences Publication Activity Database

    Procházková, Jiřina; Netíková, Jaromíra; Souček, Karel; Hofmanová, Jiřina; Kozubík, Alois

    Brno, 2006. s. 297-298. [ XXX . Brněnské onkologické dny s XX. Konferencí pro sestry a laboranty. 11.05.2006-13.5.2006, Brno] R&D Projects: GA ČR(CZ) GA524/05/0595; GA ČR(CZ) GP524/06/P345; GA AV ČR(CZ) IQS5000507 Institutional research plan: CEZ:AV0Z50040507 Keywords : differentiation * cytokines * arachidonic acid Subject RIV: BO - Biophysics

  10. Mutants of common bean alpha-amylase inhibitor-2 as an approach to investigate binding specificity to alpha-amylases Mutantes do inibidor-2 de alfa-amilase do feijão-comum para investigação da especificidade de ligação a alfa-amilases

    Directory of Open Access Journals (Sweden)

    Maria Cristina Mattar da Silva

    2004-03-01

    Full Text Available Despite the presence of a family of defense proteins, Phaseolus vulgaris can be attacked by bruchid insects resulting in serious damage to stored grains. The two distinct active forms of a-amylase inhibitors, a-AI1 and a-AI2, in P. vulgaris show different specificity toward a-amylases. Zabrotes subfasciatus a-amylase is inhibited by a-AI2 but not by a-AI1. In contrast, porcine a-amylase is inhibited by a-AI1 but not by a-AI2. The objective of this work was to understand the molecular basis of the specificity of two inhibitors in P. vulgaris (a-AI1 and a-AI2 in relation to a-amylases. Mutants of a-AI2 were made and expressed in tobacco plants. The results showed that all the a-AI2 mutant inhibitors lost their activity against the insect a-amylases but none exhibited activity toward the mammalian a-amylase. The replacement of His33 of a-AI2 with the a-AI1-like sequence Ser-Tyr-Asn abolished inhibition of Z. subfasciatus a-amylase. From structural modeling, the conclusion is that the size and complexity of the amylase-inhibitor interface explain why mutation of the N-terminal loop and resultant abolition of Z. subfasciatus a-amylase inhibition are not accompanied by gain of inhibitory activity against porcine a-amylase.Apesar de possuir uma família de proteínas de defesa, o feijão-comum (Phaseolus vulgaris L. pode ser atacado por insetos bruquídeos causando sérios danos aos grãos armazenados. O P. vulgaris possui duas formas ativas de inibidores de a-amilases, denominadas a-AI1 e a-AI2, que apresentam diferentes especificidades em relação às a-amilases. A a-amilase de Zabrotes subfasciatus é inibida por a-AI2 mas não por a-AI1. Em contraste, a a-amilase pancreática de porco é inibida por a-AI1 mas não é por a-AI2. O objetivo deste trabalho foi entender as bases moleculares da especificidade desses inibidores em relação às a-amilases. Para tanto, foram construídos mutantes do a-AI2, os quais foram expressados em plantas de fumo

  11. The frequency of β-globin gene haplotypes, α-thalassemia and genetic polymorphisms of methylenetetrahydrofolate reductase, factor V Leiden and prothrombin genes in children with sickle cell disease in Rio de Janeiro, Brazil Frequência dos haplótipos da globina beta, da talassemia alfa e dos polimorfismos genéticos dos genes da metilenotetrahidrofolato redutase, do fator V Leiden e da protrombina em crianças com doença falciforme no Rio de Janeiro, Brasil

    Directory of Open Access Journals (Sweden)

    Isaac L. Silva Filho

    2010-02-01

    Full Text Available A freqüência dos haplótipos beta S e beta C do gene da globina e a prevalência de talassemia alfa e de mutações nos genes da metilenotetrahidrofolato redutase (MTHFR-C677T, do fator V de Leiden e da protrombina (G20210A foi estudada em crianças com doença falciforme do Rio de Janeiro. O haplótipo Bantu foi o mais freqüente (65,9%, 21,2% das crianças (18% heterozigotas e 3% homozigotas apresentam talassemia com mutação alfa 3.7kb, ao contrário da mutação alfa 4.2kb que não foi encontrada. Os alelos 677CT e 677TT da MTHFR foram observados em 20,2% e 4,8%, respectivamente. Os haplótipos Camarões, Árabe-Indiano e Senegal não foram detectados na amostra estudada, bem como mutações no gene do fator V de Leiden e da protrombina. Somente o haplótipo beta C CI foi observado. Esse é o primeiro estudo realizado em uma amostra proveniente do Programa de Triagem Neonatal para Hemoglobinopatias do estado do Rio de Janeiro. Apesar do Rio de Janeiro ser a segunda maior cidade brasileira e seus habitantes expressarem o elevado grau de miscigenação ocorrida no país, nossos resultados ainda coincidem com os registros históricos dos fluxos migratórios do gene beta S para o Brasil, bem como refletem a forte influência de indivíduos de origem africana na população do Rio de Janeiro.

  12. 重组人干扰素α-2b注射液联合恩替卡韦治疗乙型肝炎e抗原阳性慢性乙型肝炎患者的疗效观察%Observation of the Curative Effect of Interferon alfa-2b Injection Combined with Entecavir in Treatment of HBeAg Positive Chronic Hepatitis B

    Institute of Scientific and Technical Information of China (English)

    张静; 李杰玉

    2015-01-01

    目的:探讨重组人干扰素α-2b注射液联合恩替卡韦治疗乙型肝炎e抗原(HBeAg)阳性慢性乙型肝炎患者的效果。方法收集48例慢性乙型肝炎患者资料,对患者使用重组人干扰素α-2b注射液联合恩替卡韦治疗,观察患者的病情变化情况。结果治疗24周后,完全应答25例,部分应答18例,无应答5例;治疗12周时,丙氨酸转氨酶(ALT)复常35例;治疗24周时,ALT复常46例。结论重组人干扰素α-2b注射液联合恩替卡韦治疗HBeAg阳性慢性乙型肝炎效果明显。%Objective To investigate the interferon alfa-2b injection combined with entecavir in treatment of HBeAg positive chronic hepatitis B effect.Methods We selected 48 cases of patients with chronic hepatitis Bdata,the use of interferon alfa-2b injection combined with entecavir treatment,observe the change of patient's condition.Results After 24 weeks of treatment,complete response in 25 cases,partial response in 18 cases,and no response in 5 cases;12 weeks of treatment,the ALT recovery in 35 cases;24 weeks of treatment,the ALT recovery in 46 cases. Conclusion Interferon alfa-2b injection combined with entecavir in treatment of HBeAg positive chronic hepatitis B effect is obvious.

  13. Perfil bioquímico do soro de frangos de corte alimentados com dieta suplementada com alfa-amilase de Cryptococcus flavus e Aspergillus niger HM2003 Biochemichal serum profile of broilers fed diets suplemented with alfa-amylase from Cryptococcus flavus and Aspergillus niger HM2003

    Directory of Open Access Journals (Sweden)

    Cibele Silva Minafra

    2010-12-01

    Full Text Available Avaliou-se o perfil bioquímico do soro de frangos de corte alimentados com a enzima α-amilase produzida por dois microrganismos. Produziram-se dois extratos, um com a-amilase obtida a partir de Cryptococcus flavus em meio de levedura comercial e outro com Aspergillus niger HM2003 em meio de proteína de soja e amido comercial, com atividade de 9,58 U/mL e 10,0 U/mL, respectivamente. Utilizaram-se 360 pintos de corte Cobb 500 de 1 dia de idade e com 49,72 ± 0,68 g de peso vivo inicial. As aves foram alojadas em baterias e foram criadas até os 21 dias de idade. Foram utilizados três dietas, cada uma com cinco repetições de 12 aves, em delineamento inteiramente casualizado. A primeira dieta (basal foi formulada sem adição de enzima e as outras duas receberam a suplementação de a-amilase produzida por cultivo de Cryptococcus flavus e Aspergillus niger HM2003. Dietas à base de milho e soja foram formuladas em duas fases: pré-inicial (1-7 dias e inicial (8-21 dias. Na fase pré-inicial, foram observados os seguintes valores médios para cálcio (6,90 e 5,99 mg/dL, proteína plasmática (2,0 e 2,50 g/dL e fosfatase alcalina (979,98 e 974,66 UI/L, respectivamente para Cryptococcus flavus e Aspergillus niger HM2003. A dieta acrescida de a-amilase obtida a partir de Aspergillus niger HM2003 determinou maior concentração sérica de fósforo. Na fase inicial, os resultados significativos relacionaram-se a potássio quando avaliadas dietas com adição de a-amilase pelas duas fontes. A incorporação das enzimas testadas não proporciona alterações metabólicas ou toxicidade nos animais.It was evaluated the biochemical serum profile of broilers fed rations supplemented with α-amylase produced by two microorganisms. Two extracts were produced, one was produced with a-amylase obtained from Cryptococcus flavus in a commercial yeast-based medium and the other with Aspergillus niger HM2003 produced in soybean protein and commercial starch medium

  14. Correlation between natural killer T cell expression and virological response to treatment with peg-interferon alfa-2a in patients with HBeAg-positive chronic hepatitis B%聚乙二醇干扰素治疗HBeAg阳性慢性乙型肝炎患者外周血NKT细胞的变化及疗效预测

    Institute of Scientific and Technical Information of China (English)

    黄芳; 龚环宇; 鲁猛厚

    2013-01-01

    目的 研究聚乙二醇干扰素α-2a(Peg-INFα-2a)治疗乙型肝炎e抗原(HBeAg)阳性慢性乙型肝炎(CHB)患者,其外周血NKT细胞表达率和疗效预测相关性.方法 选取 2010年1-12月在中南大学湘雅三医院住院和门诊接受治疗的HBeAg阳性CHB患者63例,予Peg-INFα-2a 18 MIU肌内注射,1次/周,共48周.检测各时段外周血NKT数量占外周血T淋巴细胞的百分比、血清乙型肝炎5项定量及乙型肝炎病毒(HBV) DNA载量.结果 Peg-INFα-2a治疗HBeAg阳性CHB患者48周后,显效(完全病毒学应答)26例,有效(部分病毒学应答)21例,无效(无病毒学应答)16例.外周血NKT 细胞占T淋巴细胞百分比:显效组治疗前及治疗后4、8、12、16、24周,较有效组和无效组均明显升高(均P<0.01);在治疗48周和停药24周,显效组较有效组NKT表达水平明显升高(分别t=32.0,P<0.01;t=27.6,P<0.01).显效组治疗后前4周,NKT表达水平上升速度最快,12周时达最高峰,其后逐渐下降,到24周比治疗前水平稍高,一直维持到48周;有效组12周时NKT表达水平达最高峰,较治疗前显著增高(t=12.83,P<0.05).显效组患者肝功能基本在12周左右完全恢复正常,且持续维持在正常水平,其HBV DNA载量亦逐步下降;而有效组和无效组患者的肝功能波动在(1~2)×ULN.监测至停药24周后,共有27例患者出现HBeAg血清学转换.结论 Peg-INFα-2a治疗HBeAg阳性CHB患者,其外周血中NKT的表达对疗效有一定预测作用.%Objective To study the correlation between expression of natural killer T (NKT) cells and virological response to treatment with peg-interferon alfa-2a (Peg- INFα-2a)in patients with HBeAg-positive chronic hepatitis B (CHB). Methods A cohort of 63 HBeAg-positive CHB inpatients and outpatients in a hospital between January and December 2010 were treated with 18MIU Peg-IFNα-2a once a week lor 48 weeks. The percentage of NKT cells in T lymphocytes, five serological markers of

  15. Quantifiable changes in HBeAg expression predict therapeutic efficacy of peg-interferon alfa-2a in patients with HBeAg-positive chronic hepatitis B%HBeAg早期下降对聚乙二醇干扰素α-2a治疗HBeAg阳性慢性乙型肝炎的疗效预测

    Institute of Scientific and Technical Information of China (English)

    纪永健; 李菲菲; 任万华; 主余华; 秦成勇

    2013-01-01

    Objective To investigate whether quantifiable changes in serum levels of hepatitis Be antigen (HBeAg) in response to 24 weeks of pegylated-interferon alfa-2a (Peg-IFN-α 2a) treatment are predictive of therapeutic efficacy at 48 weeks of treatment in HBeAg-positive chronic hepatitis B (CHB)patients and to investigate the efficacy of using an individualized antiviral treatment strategy.Methods Ninety-six HBeAg-positive CHB patients with detectable HBeAg at week 24 of Peg-IFN-α 2a treatment were categorized according to the quantitative change in HBeAg (vs.pre-treatment baseline):group A,HBeAg decline > 2 log; group B,HBeAg decline between 1~2 log; group C,HBeAg decline < 1 log,which was then randomly divided into two sub-groups:C1 and C2.Group A,B,and C1 patients continued the original therapy for an additional 24 weeks,while group C2 patients were supplemented with lamivudine (3TC + Peg-IFN-α 2a) for the additional 24 weeks of treatment.All patients underwent liver biopsy at the end of treatment (week 48),and HBV covalently-closed circular (ccc)DNA was quantified as a measure of therapeutic efficacy.A,B,and C1 between-group multiple comparisons were made by the Nemenyi test; C1 and C2 between-group comparison was made by the Mann-Whitney U test.The significance of between-group differences in decreased HBV cccDNA vs.HBeAg/anti-HBe seroconversion was made by the Chi-squared test.Results At week 48,the mean decrease of serum HBV cccDNA in each group was:A,5.8 log10 copy/ml; B,3.8 log10copy/ml; C1,2.8 log10 copy/ml; C2,5.7 log10 copy/ml.Statistically significant differences were observed for group A vs.B and C1 (P < 0.01) and C1 vs.C2 (P < 0.01); however,the difference between group B and C1 did not reach statistical significance (P =0.19).The mean decrease of HBeAg in each group was:A,2.7 log10 S/CO; B,1.9 log10 S/CO; C1,0.9 log10 S/CO; C2,1.6 log10 S/CO.Statistically significant differences were observed for group A vs.B and C1 (P < 0.01) and C1 vs.C2

  16. Autoimmune hemolytic anemia in HCV/HIV coinfected patients during treatment with pegylated alpha-2a interferon plus ribavirin Anemia hemolítica auto-imune durante o tratamento da hepatite crônica com interferon peguilado alfa 2a e ribavirina, em pacientes com co-infecção HCV/HIV

    Directory of Open Access Journals (Sweden)

    Fernando Crivelenti Vilar

    2007-12-01

    Full Text Available Two cases of autoimmune hemolytic anemia that occurred during the treatment of chronic hepatitis C with pegylated alpha-2a interferon and ribavirin, in HIV coinfected patients, are presented and described. The late occurrence (after six months of therapy of this severe hemolytic anemia leads to the recommendation that hemoglobin levels should be monitored throughout the treatment period, even among patients who presented stable hemoglobin levels in the preceding months.São apresentados e discutidos dois casos de anemia hemolítica auto-imune que ocorreram durante o tratamento da hepatite crônica pelo vírus C, com interferon peguilado alfa 2a e ribavirina, em pacientes co-infectados pelo HIV. A ocorrência de anemia hemolítica grave em etapa tardia, após o sexto mês da terapêutica, recomenda que o controle dos níveis de hemoglobina deva ser feito durante todo o período do tratamento , mesmo nos pacientes que apresentam níveis estáveis de hemoglobina nos meses precedentes.

  17. Evaluation of total carotenoids, alpha- and beta-carotene in carrots (Daucus carota L. during home processing Avaliação de carotenóides totais, alfa e beta-caroteno em cenoura (Daucus carota L. durante processamento a nível doméstico

    Directory of Open Access Journals (Sweden)

    Helena Maria PINHEIRO-SANT’ANA

    1998-04-01

    Full Text Available This study aims to analyze the influence of dehydration and different preparation methods during home processing related toalpha-carotene, beta-carotene and total carotenoids stability in carrots. Vitamin A values were evaluated after different treatments. Thus, carrots were submitted to steam cooking, water cooking with and without pressure, moist/dry cooking and conventional dehydration. Determination of alpha- and beta-carotenes was made by High-Performance Liquid Chromatography (HPLC (conditions were developed by us using spectrophotometric detection visible-UV at 470 nm; a RP-18 column and methanol: acetonitrile: ethyl acetate (80: 10: 10 as mobile phase. Total carotenoids quantification was made by 449 nm spectrophotometer. The retention of the analyzed carotenoids ranged from 60.13 to 85.64%. Water cooking without pressure promoted higher retention levels of alpha- and beta-carotene and vitamin A values, while water cooking with pressure promoted higher retention levels of total carotenoids. Dehydration promoted the highest carotenoid losses. The results showed that, among the routinely utilized methods under domestic condition, cooking without pressure, if performed under controlled time and temperature, is the best method as it reduces losses in the amount of alpha- and beta-carotene, the main carotenoids present in the carrots. Despite the significant carotenoid losses, carrots prepared through domestic methods, remain a rich source of provitamin A.O presente estudo teve como objetivo analisar a influência da desidratação e de diferentes métodos de preparo a nível doméstico sobre a estabilidade de alfa-caroteno, beta-caroteno e carotenóides totais em cenouras. Os valores de vitamina A foram avaliados após os diferentes tratamentos. Para tanto, amostras de cenoura foram submetidas à cocção a vapor, cocção em água com e sem pressão, cocção úmida/seca e à desidratação convencional. Para a determinação de alfa e beta

  18. Development and application of hepatitis C reporter viruses with genotype 1 to 7 core-nonstructural protein 2 (NS2) expressing fluorescent proteins or luciferase in modified JFH1 NS5A

    DEFF Research Database (Denmark)

    Gottwein, Judith M; Jensen, Tanja B; Mathiesen, Christian K;

    2011-01-01

    To facilitate genotype-specific high-throughput studies of hepatitis C virus (HCV), we have developed reporter viruses using JFH1-based recombinants expressing core-nonstructural protein 2 (NS2) of genotype 1 to 7 prototype isolates. We introduced enhanced green fluorescent protein (EGFP) into NS5A...... deletions in EGFP, while 2a(J6)¿40 did not show an impaired viability. We further developed panels of JFH1-based genotype 1 to 7 core-NS2 recombinants expressing EGFP- or RLuc-NS5A¿40 fusion proteins. In cell culture, the different EGFP recombinants showed growth characteristics comparable to those...... of these reporter viruses for high-throughput fluorescence- and luminescence-based studies of HCV-receptor interactions and serum-neutralizing antibodies was demonstrated. Finally, using RLuc viruses, we showed that the genotype-specific core-NS2 sequence did not influence the response to alfa-2b interferon (IFN...

  19. Comparison of nuclear data of 64Cu production using an accelerator by TALYS 1.0 code

    Directory of Open Access Journals (Sweden)

    Sadeghi Mahdi

    2010-01-01

    Full Text Available The extensive use of 64Cu (T1/2 = 12.7 h as a positron and electron emitter radioisotope in recent years has ensured its potential to serve a dual role in the development of molecular agents in PET and radioimmunotheraphy drugs in oncology. The TALYS 1.0 code was used to calculate excitation functions for induced proton, deuteron and alfa-particles on 64Zn, 66Zn, 67Zn, 68Zn, 70Zn, 62Ni, and 64Ni up to 50 MeV. According to the data acquired by the TALYS 1.0 code, thick-target integral yield of the induced charged particles on the enriched targets was achieved.

  20. Effect of Human Interferon Alfa-2b and Cyclooxygenase-2 Inhibitor on Cells of Hepatocarcinoma%联合应用干扰素α-2b及选择性环氧化酶2抑制剂对肝癌细胞抑制作用的研究

    Institute of Scientific and Technical Information of China (English)

    孙忠志; 陈剑群

    2011-01-01

    目的 观察干扰素α-2b(IFN-α-2b),选择性环氧化酶2(COX-2)抑制剂非甾体类消炎药塞来昔布单用以及两者联合应用时,对人肝癌细胞株HepG2的生长抑制作用及同时检测药物作用后COX-2和血管内皮生长因子(VEGF)及Bcl-2表达的情况,探讨药物作用的机制.方法 HepG2细胞根据所加药物不同分为不同浓度的IFN-α-2b组(1250、2500、5000、10 000、20 000、40 000 U/mL)、塞来昔布组(25、50、100、200 μmol/L)、联合用药组(IFN-α-2b 5000 U/mL+塞来昔布 50 μmol/L、IFN-α-2b 5000 U/mL+选择性COX-2 100 μmol/L).MTT法检测不同时间(24、48 h)各组细胞增殖抑制率,流式细胞术检测细胞凋亡率,Western blot检测用药前后COX-2、VEGF、Bcl-2蛋白在HepG2细胞中的表达变化.结果 不同浓度的塞来昔布和干扰素对HepG2细胞均有抑制作用,且联合效果明显优于单用,各组与对照组差异有统计学意义(P<0.05);流式细胞术分析:塞来昔布50 μmol/L组、塞来昔布100 μmol/L组、IFN-α-2b 5000 U/mL组、IFN-α-2b 5000 U/mL+塞来昔布50 μmol/L组、IFN-α-2b 5000 U/mL+塞来昔布100 μmol/L组细胞凋亡率分别为(14.93±0.79)%、(25.43±0.89)%、(20.52±1.46)%、(27.12±3.34)%、(48.17±2.04)%,与阴性对照组(8.73±0.83)%比较差异均有统计学意义(P<0.05);塞来昔布单用及联合IFN-α-2b作用48 h后,各组COX-2、VEGF、Bcl-2蛋白的表达下调,呈剂量依赖性,联合用药组较单药组作用明显.结论 干扰素α-2b和塞来昔布均有抑制肝癌细胞HepG2增殖的作用,同时对细胞具有凋亡诱导作用,亦能抑制细胞中COX-2、VEGF、Bcl-2的表达.上述抑制作用随药物浓度的增加、作用时间的延长而增强,联合作用更强.%Objective To investigate the growth inhibition and its molecular mechanism on human hepatoma cell line HepG2 through application of celecoxib, cyclooxygenase-2 inhibitor, interferon alfa-2b alone or in combination with each other,and test

  1. 干扰素α-1b治疗慢性乙肝疗效10年随访

    Institute of Scientific and Technical Information of China (English)

    吴群; 刘小兵

    2013-01-01

    目的观察a-1b干扰素抗乙肝病毒远期疗效。方法选择1999~2002年我科治疗的152例慢性乙肝患者,其中治疗组76例,应用a-1b干扰素5MIU肌肉注射3次/w,疗程12~24w;对照组76例仅用一般保肝降酶药物,疗程4~12w,治疗结束后长期随访血生化指标、病毒学指标、病毒载量、影像学检查等。结果治疗结束时和随访1年、5年、10年时a-1b干扰素治疗组乙肝病毒载量(HBVDNA)阴转率分别为47.4%、52.6%、44.7%、47.0%,干扰素治疗组临床预后、病情稳定率、发生肝硬化、肝癌及肝病死亡情况明显好于对照组相关指标(P<0.01)。结论 a-1b干扰素抗乙肝病毒疗程较现有抗病毒药物短,疗效确切,远期稳定性较高,同时能减少肝病复发,进而减少肝硬化、肝癌等的发生。%Objective To investigate the Long-term Therapeutic Ef ects of Interferon alfa-1b against HBV. Methods 152 cases of chronic hepatitis B patients receiving treatments between 1999 and 2002 are selected,76 patients in treatment group (control group) receive 5MIU Interferon alfa-1b therapy three times every week by Intramuscular Injection for a course of 12 to 24 weeks, while the control group of 45 patients is only given with conventional liver protecting and enzyme decreasing drug for a course of 4 to 12 weeks, long-term fol ow up of blood biochemical indexes, virology indexes, viral load and imaging examination etc after therapy. Results at the end of treatment,1 year,5 years and 10 years after treatment, HBVDNA negative rate with Interferon alfa-1b therapy group is 47.4%,52.6%,44.7%and 47.0%respectively, there is an obvious improvement on the clinical prognosis, condition maintaining rate, and prevention of cirrhosis, liver cancer and death in the treatment group over that in the control group (p<0.01). Conclusion Interferon alfa-1b not only decreases the course needed by normal anti-virus drugs and shows significant ef ects against

  2. Histological and biochemical serum effects of alpha-tocopherol on ischemia/reperfusion-related injuries induced in the pelvic limb of rats Efeitos histológicos e bioquímicos séricos do alfa-tocoferol na lesão de isquemia e reperfusão em membro pélvico de ratos

    Directory of Open Access Journals (Sweden)

    Marcelo Gomes da Silva

    2005-10-01

    Full Text Available PURPOSE: To evaluate the protective action of alpha-tocopherol in ischemia/reperfusion injuries of pelvic member of rats. METHODS: Thirty adult male rats of the Wistar strain were randomized into three experimental groups of 10: Group I - control group with no ischemia or reperfusion. Groups II and III - four hours of ischemia and of hours of reperfusion by means of clamping of the infrarenal aorta. The animals of Group II were treated with saline and those of Group III were treated with i.v. alpha-tocopherol (50 mg/kg. Parameters studied were biopsies of the soleus muscle, dosing of creatine phosphokinase, lactate dehydrogenase, potassium, calcium and arterial blood gasometry. RESULTS: The results of biopsies of the soleus muscles studied by optical microscopy, were not significant in terms of presence of edema among the three groups studied. Variables inflammation and necrosis were not observed, therefore cannot be statistically analyzed. As to dosing of calcium and lactate dehydrogenase, the pH, pO2 and pCO2 values were not significant for all groups studied. We observed that the levels of potassium (Group II > Group I, Fcalculated = 5.84; Fcritical = 3.33, creatine phosphokinase (Group II > Groups I and III, Hcalculated = 13.92; Hcritical = 5.99 and bicarbonate (Groups I and III > Group II, Hcalculated = 11.98; Hcritical = 5.99 presented significant results among groups. CONCLUSION: From the serum biochemical perspective, the treatment with alpha-tocopherol has attenuated the metabolic injuries in the ischemia/reperfusion syndrome in this experimental model.OBJETIVO: Avaliar ação protetora do alfa-tocoferol na lesão de isquemia e reperfusão em membro pélvico de ratos. MÉTODOS: Trinta ratos machos adultos da linhagem wistar foram distribuídos aleatoriamente, em três grupos experimentais, com 10 animais cada: Grupo I - Grupo controle sem isquemia ou reperfusão. Grupos II e III - quatro horas de isquemia e duas horas de reperfus

  3. A randomized controlled trial adding fluvastatin to peginterferon and ribavirin for naïve genotype 1 hepatitis C patients.

    Science.gov (United States)

    Bader, T; Hughes, L D; Fazili, J; Frost, B; Dunnam, M; Gonterman, A; Madhoun, M; Aston, C E

    2013-09-01

    Fluvastatin or simvastatin has demonstrable antiviral activity against hepatitis C virus (HCV) as monotherapy. The safety and efficacy of adding fluvastatin or simvastatin to peginterferon/ribavirin for 48 weeks was tested in HCV genotype 1 naïve-to-treatment veterans. Thirty-seven naïve-to-treatment genotype 1 HCV patients were randomized to either a control group (n = 20) to receive peginterferon alfa plus ribavirin or an experimental group (n = 18) to similarly receive peginterferon alfa plus ribavirin as well as fluvastatin 20 mg/day. In addition, seven patients who presented for HCV treatment already were on simvastatin and could not be withdrawn. These simvastatin users were not randomized but were entered into a concurrent prospective pilot arm. There were no unique safety issues with fluvastatin or simvastatin when these drugs were given with peginterferon/ribavirin for 48 weeks. Thirteen of 25 statin patients achieved sustained viral response (SVR), while 5 of 20 control patients achieved SVR. Analysis of SVR by intention-to-treat showed P = 0.078. In this phase 2 study, there were no safety issues with the addition of fluvastatin or simvastatin to peginterferon and ribavirin for 48 weeks. There was a trend towards improvement in SVR when fluvastatin or simvastatin was administered with peginterferon/ribavirin. The size of the groups did not reach the prestudy size thought needed to show significant difference (type II error). These results support the significant results of two other larger randomized controlled trials reported using the same dose of fluvastatin in naïve-to-treatment genotype 1 HCV patients. PMID:23910646

  4. 重组干扰素α-2a和α-2b治疗慢性乙型肝炎疗效比较%Interferon Alfa in the Treatment of Chronic Hepatitis B

    Institute of Scientific and Technical Information of China (English)

    武宗导; 王功大; 许洁; 邢国珍

    2002-01-01

    @@ 应用分子重组技术制备的α干扰素,具有天然α干扰素同样的抗病毒、调控免疫、抗增殖的特性[1],国内外已广泛用于治疗病毒性肝炎.我院自1999年1月到2001年6月应用不同亚型国产重组干扰素α-2a和α-2b治疗慢性乙型肝炎病人,对比观察不同亚型α干扰素抗病毒效应,报告如下.

  5. Mutations in nonstructural 5A gene of hepatitis C virus and its response to interferon alfa%丙型肝炎病毒NS5A基因变异与干扰素疗效的关系

    Institute of Scientific and Technical Information of China (English)

    张琳; 赵桂珍; 石理兰; 曹丽

    2003-01-01

    目的:探讨HCV1b型慢性丙型肝炎患者HCV NS5A基因变异与干扰素(IFN)疗效的关系,NS5A2209-2248片断是否存在干扰素敏感决定区(ISDR).方法:留取慢性丙型肝炎患者干扰素治疗前血清,应用RT-PCR法扩增NS5A基因片段,用直接测序法进行核苷酸及氨基酸序列测定.结果:11例HCV1b型患者中1例为中间型,其余为野生型.2例表现为完全应答,均为野生型,9例为无应答.两组之间核苷酸及氨基酸序列无显著差异.对1例患者的动态观察发现,干扰素治疗后其核苷酸及氨基酸序列均有所变化,改变了其在基因树中的位置.结论:HCVlb型NS5A基因变异与干扰素疗效无关,NS5A2209-2248区高度保守.未证实存在ISDR.干扰素治疗可引起HCV准种改变.

  6. Vliv TNF-alfa na genotoxické účinky benzo(a)pyrenu v jaterních progenitorových buňkách

    Czech Academy of Sciences Publication Activity Database

    Umannová, Lenka; Machala, M.; Kozubík, Alois; Topinka, J.; Nováková, Zuzana; Vondráček, Jan

    Olomouc, 2007. s. 53. ISBN 978-80-244-1730-1. [Konference vědeckých prací studentů DSP. 10.09.2007-11.09.2007, Olomouc] R&D Projects: GA ČR(CZ) GA524/05/0595 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702; CEZ:AV0Z50390512; CEZ:AV0Z50390703 Keywords : environmental pollutants * apoptosis * genotoxic effects Subject RIV: BO - Biophysics

  7. Artvin, Rize ve Trabzon’daki Doğal Kaynak Sularında Toplam Alfa, Toplam Beta Aktivite Değerlerinin Belirlenmesi ve Jeoistatistiksel Olarak Modellenmesi

    OpenAIRE

    Kobya, Yaşar; Yeşilkanat, Cafer Mert; Çakır, Bakiye

    2014-01-01

    Trabzon, Artvin and Rize where being the most northeast of the Turkey are three cities take place an area approximately 18000 km2 large. Samples was collected from totally 20 potable water supplies into the cities and their gross alpha and gross beta activity values were measured with Berthold LB770 marked alpha, beta counting device. Activity values of the samples were evaluated value ranges 1.7- 114.2 Bq/L for gross alpha and 2.9-90.8 Bq/L for gross beta. The results were compared with worl...

  8. Measure of exposure of short-lived radon products using an alpha spectrometer for measuring indoor aerosol activity concentration and dose evaluation; Misure di esposizione ai prodotti di decadimento del radon a breve vita tramite uno spettrometro alfa per la misura dell'attivita' del particolato atmosferico indoor e valutazioni dosimetriche

    Energy Technology Data Exchange (ETDEWEB)

    Berico, M.; Castellani, C.M.; Formignani, M. [ENEA, Divisione Protezione dell' Uomo e degli Ecosistemi, Centro Ricerche Ezio Clementel, Bologna (Italy); Mariotti, F. [Bologna Univ., Bologna (Italy). Dipt. di Fisica

    2001-07-01

    A new italian law introduces the regulation of occupational exposure to radon. To evaluate the inhalation of radon daughters by the workers a sampling device has been assembled with the aim of evaluation of unattached and aerosol attached radon daughters' fractions. The instrument, based on selection of the aerosuspended particles by means of a wire screen type battery and subsequent collection on a total filter, allows to describe the behaviour of both fractions using defined temporal pattern of collecting particles and counting them by alpha spectroscopy. A measurement campaign to test the radon daughter dichotomous spectrometer in comparison with a commercial Radon Working Level meter, has been performed in a research laboratory of central Italy affected by high radon concentrations. The radon concentration during the measurement campaign has been also measured. The equilibrium factor F{sub e}q ad the attachment factor fp have been evaluated during 3 days campaign. Using the measured mean parameters (radon concentration, F{sub e}q, f{sub p}) the dose evaluation for workers using dosimetric approach has been performed. A comparison between the epidemiologic approach, based on the radon concentration, and dosimetric approach is also presented. [Italian] L'esposizione a radon in ambiente lavorativo e la conseguente inalazione dei suoi prodotti di decadimento in forma particolata e' oggetto di una recente normativa italiana in materia di protezione dalle radiazioni ionizzanti. Per rispondere a questa necessita', presso l'Istituto per la Radioprotezione dell'ENEA di Bologna e' stato progettato e realizzato uno spettrometro alfa per la misura della progenie del radon con la finalita' di valutare, su brevi periodi di tempo, la concentrazione individuale dei suoi prodotti di decadimento e, con l'impiego di batterie a diffusione a reti, consentire inoltre la discriminazione della concentrazione della frazione attaccata e non

  9. Relato do primeiro paciente brasileiro com a forma infantil da doença de Pompe tratado com alfa-glicosidase recombinante humana Report of the first Brazilian infantile Pompe disease patient to be treated with recombinant human acid alpha-glucosidase

    Directory of Open Access Journals (Sweden)

    Sandra J. Pereira

    2008-06-01

    Full Text Available Objetivo: Relatar o primeiro caso de forma infantil da doença de Pompe tratado no Brasil. Descrição: Trata-se de doença de depósito lisossomal que se caracteriza por defeitos da enzima alfa-glicosidase ácida, com acúmulo intracelular de glicogênio, principalmente nos músculos. São descritas a forma infantil e tardia. Desde 2006, está disponível tratamento com enzima recombinante humana. Descreve-se o primeiro caso de forma infantil da doença tratado no Brasil. Trata-se de menina com 2,5 meses de idade e progressão rápida da doença, com perda dos movimentos dos membros, miocardiopatia hipertrófica e insuficiência respiratória aos 7 meses de idade. Após 10 meses de tratamento, apresentou boa resposta clínica, com remissão da insuficiência respiratória, recuperação parcial dos movimentos dos membros e melhora importante do quadro cardiológico. Comentários: Apesar de pouco freqüente, a forma infantil da doença de Pompe é letal. A disponibilidade de tratamento eficaz aumenta a necessidade de conhecimento e diagnóstico precoce da doença.Objective: To describe the first case of infantile Pompe disease to be treated in Brazil. Description: Pompe disease is a glycogen storage disease related to defects in the acid alpha-glucosidase enzyme, leading to an intracellular accumulation of glycogen, mainly in muscles. Two forms are described: infantile and juvenile. Since 2006, treatment with recombinant human acid alpha-glucosidase has been available. This article describes the first case of infantile Pompe disease treated in Brazil. A girl presented at 2.5 months of age with rapid disease progression, exhibiting severe hypotonia, loss of movements in both upper and lower limbs and hypertrophic cardiomyopathy, progressing to respiratory failure by the age of 7 months. After 10 months of treatment, she exhibited a good clinical response, with remission of the respiratory failure, partial recovery of arm and leg movements and

  10. Inhibitory effect of beta-pinene, alpha-pinene and eugenol on the growth of potential infectious endocarditis causing Gram-positive bacteria Efeito inibitório de eugenol, beta-pineno e alfa-pineno sobre o crescimento de bactérias Gram-positivas potencialmente causadoras de endocardite infecciosa

    Directory of Open Access Journals (Sweden)

    Aristides Medeiros Leite

    2007-03-01

    Full Text Available This study was led with the purpose of evaluating the effectiveness of eugenol, beta-pinene and alpha-pinene in inhibiting the growth of potential infectious endocarditis causing gram-positive bacteria. The phytochemicals Minimum Inhibitory Concentration-MIC was determined by solid medium diffusion procedure, while the interference of the MIC values on the bacterial cell viability was performed by viable cells count. Staphylococcus aureus, S. epidermidis, Streptococcus pneumoniae and S. pyogenes strains were used as test microorganisms. The assayed phytochemicals showed effectiveness in inhibiting all assayed bacteria strains presenting MIC values between 2.5 and 40 µL/mL. Eugenol showed the lowest MIC values which were between 2.5 and 5 µL/mL for the most bacteria strains. MIC values found to the phytochemicals were able to inhibit the cell viability of S. aureus providing a total elimination of the bacteria inoculum in a maximum time of 24 hours of exposure. These data showed the interesting antibacterial property of the assayed phytochemicals and support their possible and rational use in the antimicrobial therapy.Este estudo foi conduzido com a proposta de avaliar a efetividade de eugenol, beta-pineno e alfa-pineno em inibir o crescimento de cepas de bactérias Gram-positivas potencialmente causadoras de endocardite infecciosa. A Concentração Inibitória Mínima-CIM dos fitoconstituintes foi determinada através do método de difusão em meio sólido, enquanto a interferência da CIM sobre a viabilidade celular bacteriana foi avaliada através da contagem de células viáveis. Cepas de Staphylococcus aureus, S. epidermidis, Streptococcus pneumoniae e S. pyogenes foram utilizadas como microrganismos teste nos ensaios antimicrobianos. Os fitoconstituintes ensaiados mostraram efetividade em inibir todas as cepas bacterianas utilizadas como microrganismos testes apresentando valores de CIM entre 2.5 e 40 µL/mL. Eugenol apresentou os menores

  11. Alpha radioactivity monitoring related to Radon-222 in water from wells in metropolitan area of Curitiba (PR), Brazil; Monitoramento da radioatividade alfa relacionada ao radonio-222 em aguas de pocos da regiao metropolitana de Curitiba (PR)

    Energy Technology Data Exchange (ETDEWEB)

    Correa, Janine Nicolosi; Paschuk, Sergei Anatolyevich; Kappke, Jaqueline; Claro, Flavia Del; Perna, Allan Felipe Nunes; Reque, Marilson, E-mail: janine_nicolosi@hotmail.com [Universidade Tecnologica Federal do Parana (UTFPR), Curitiba, PR (Brazil); Denyak, Valeriy; Schelin, Hugo Reuters [Instituto de Pesquisa Pele Pequeno Principe (IPPPP), Curitiba, PR (Brazil); Rocha, Zildete; Santos, Talita Oliveira [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil)

    2015-04-15

    This research objective was to assess the level of randon-222 concentration in well water of the metropolitan region of Curitiba, Parana. Current work presents the results of indoor {sup 222}Rn activity ground water samples from artesian wells from aquifers of the region. The studies of radon activity in water were performed using the radon detector AlphaGUARD. The calculations of initial radon activity in water were done considering the {sup 222}Rn decay correction as well as equilibrium level of {sup 222}Rn and {sup 226}Ra observed after 30 days of measurements. Obtained results show that about 70% of measured activity levels of {sup 222}Rn are higher than the recommended value of 11.1 Bq.L{sup -1}, which represent the risk for the human health associated with this radionuclide. The case study showed that previous measurements of radon are recommended for a construction project is implemented. In this case, it is observed that the radon concentrations decrease about 56% in the first water tank and 83% in the second water tank over the well. This fact shows that the actions for mitigation of radon are viable and do not require major modifications to the usual systems of construction. (author)

  12. Upregulated expression of human neutrophil peptides 1, 2 and 3 (HNP 1-3 in colon cancer serum and tumours: a biomarker study

    Directory of Open Access Journals (Sweden)

    Olsen Jesper

    2005-01-01

    Full Text Available Abstract Background Molecular markers for localized colon tumours and for prognosis following therapy are needed. Proteomics research is currently producing numerous biomarker studies with clinical potential. We investigate the protein composition of plasma and of tumour extracts with the aim of identifying biomarkers for colon cancer. Methods By Surface Enhanced Laser Desorption/Ionisation – Time Of Flight / Mass spectrometry (SELDI-TOF/MS we compare the protein profiles of colon cancer serum with serum from healthy individuals and the protein profiles of colon tumours with normal colon tissue. By size exclusion chromatography, we investigate the binding of HNP 1-3 to high mass plasma proteins. By microflow we investigate the effect of HNP 1-3 on mammalian cells. Results Human Neutrophil Peptides -1, -2 and -3 (HNP 1-3, also known as alfa-defensin-1, -2 and -3, are present in elevated concentrations in serum from colon cancer patients and in protein extracts from colon tumours. A fraction of HNP 1-3 in serum is bound to unidentified high mass plasma proteins. HNP 1-3 purified from colon tumours are lethal to mammalian cells. Conclusions HNP 1-3 may serve as blood markers for colon cancer in combination with other diagnostic tools. We propose that HNP 1-3 are carried into the bloodstream by attaching to high mass plasma proteins in the tumour microenvironment. We discuss the effect of HNP 1-3 on tumour progression.

  13. Upregulated expression of human neutrophil peptides 1, 2 and 3 (HNP 1-3) in colon cancer serum and tumours: a biomarker study

    International Nuclear Information System (INIS)

    Molecular markers for localized colon tumours and for prognosis following therapy are needed. Proteomics research is currently producing numerous biomarker studies with clinical potential. We investigate the protein composition of plasma and of tumour extracts with the aim of identifying biomarkers for colon cancer. By Surface Enhanced Laser Desorption/Ionisation – Time Of Flight / Mass spectrometry (SELDI-TOF/MS) we compare the protein profiles of colon cancer serum with serum from healthy individuals and the protein profiles of colon tumours with normal colon tissue. By size exclusion chromatography, we investigate the binding of HNP 1-3 to high mass plasma proteins. By microflow we investigate the effect of HNP 1-3 on mammalian cells. Human Neutrophil Peptides -1, -2 and -3 (HNP 1-3), also known as alfa-defensin-1, -2 and -3, are present in elevated concentrations in serum from colon cancer patients and in protein extracts from colon tumours. A fraction of HNP 1-3 in serum is bound to unidentified high mass plasma proteins. HNP 1-3 purified from colon tumours are lethal to mammalian cells. HNP 1-3 may serve as blood markers for colon cancer in combination with other diagnostic tools. We propose that HNP 1-3 are carried into the bloodstream by attaching to high mass plasma proteins in the tumour microenvironment. We discuss the effect of HNP 1-3 on tumour progression

  14. Prolonged duration of the routine pegylated-interferon alfa-2a therapy produces superior virological response in HBeAg-positive chronic hepatitis B patients: a single-center cohort study%聚乙二醇干扰素α-2a治疗HBeAg阳性慢性乙型肝炎72周疗程的疗效优于常规疗程

    Institute of Scientific and Technical Information of China (English)

    朱月永; 吴银莲; 陈靖; 郑琦; 董菁; 江家骥

    2012-01-01

    Objective Hepatitis B e antigen (HBeAg) seroconversion and/or hepatitis B surface antigen (HBsAg) clearance are considered as good prognostic indicators of treatment outcome in HBeAg-positive chronic hepatitis B (CHB) patients.While a sustained virological response (SVR) can be achieved by a finite 48-week course of pegylated-interferon alfa-2a (Peg-IFNα-2a),it has been suggested that longer-term treatment can improve the rate of SVR.Therefore,the aim of this study was to compare the effects of prolonged and routine Peg-IFNα-2a therapy in patients with HBeAg-positive CHB.Methods Eightysix consecutive patients diagnosed with HBeAg-positive CHB at our hospital between September 2006 and October 2009 were enrolled in the study.The patients were randomly assigned to receive Peg-IFNα-2a (180 μg once weekly) for either 48 weeks (routine therapy group,n =53) or 72 weeks (prolonged therapy group,n =33).Serum samples were collected from each patient every three months until the end of the 24-week follow-up,and standard viral and biochemical tests were carried out.Relapse was defined as HBV DNA concentrations > 105 copies/mL or an HBeAg-positive test at the end of the 24-week follow-up.Chi-squared test and the t-test were used to determine the significance of intergroup differences.Logistic regression analysis was employed to determine the correlation of outcome parameters to treatment duration,expressed as odds ratio (OR) with 95% confidence interval (CI).Results The two treatment groups were similar at baseline (pre-treatment) in demographic data,sex ratio,age,alanine aminotransferase (ALT) level,HBV DNA load,and semi-quantitative level of HBeAg (s/co) (all,P> 0.05).At the end of the 24-week follow-up,there were significant differences between the 48-week treatment group and the 72-week treatment group in patients with HBV DNA negativity (62.3% vs.97.0%,x2=13.273,P=0.000),HBeAg seroconversion (39.6% vs.57.6%,x2 =6.765,P =0.009),HBsAg clearance (15.1% vs

  15. Determination of total alpha and beta activity in water for human consumption by LSC(Liquid Scintillation Counter); Determinacao de atividades alfa e beta total em agua para consumo humano por LSC (Contador de Cintilacao Liquida))

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2013-07-01

    The Ordinance Brazilian of Ministry of Health (MS 2914/2011) establishes the standards for quality of water intended for human consumption, being limits values of 5.0 Bq/L for gross alpha, and 1.0 Bq/L for gross beta radioactivity. The liquid scintillation spectrometry (LSC) technique has been presented as an alternative to conventional procedure using gas flow proportional counter. The present work shows a review of the methods for determination of gross alpha and gross beta in water by using LSC. Between the factors that influence the accuracy and repeatability of the analytical results we can highlight: thermal preconcentration, type of the acid and calibration standard. A procedure was established and carried out to samples of the National Program of Intercomparison of Radionuclides in Environmental Samples for evaluation of its performance. The gross alpha and gross beta analysis in samples of the public water supplies in the Metropolitan Region of Goiania, state of Goias was carried out. The results are consistent with the guideline values form the Ministry of Health concerning radioactivity. (author)

  16. Sequenciamento e análise dos genes das subunidades alfa e beta do hormônio folículo estimulante de bovino (Bos taurus indicus Sequencing and analysis of subunits alpha and beta of the follicle stimulating hormone from bovine (Bos taurus indicus

    Directory of Open Access Journals (Sweden)

    Luci Sayori Murata

    2008-07-01

    Full Text Available

    Este trabalho relata uma clonagem e seqüenciamento das subunidades alfa e beta do hormônio folículo estimulante de Bos taurus indicus. Também apresenta os resultados de comparação realizada das seqüências gênicas dessas cadeias com as seqüências das cadeias alfa e beta do FSH de suínos e da cadeia beta de bovinos Bos taurus taurus já presentes no GenBank. Na comparação das seqüências de nucleotídeos e de aminoácidos predita da cadeia αFSH de Bos taurus indicus com as cadeias αFSH de outras espécies como suínos e búfalo (Bubalis bubalis, observou-se que as seqüências são bastante similares. A comparação da seqüência da subunidade αFSH de Bos taurus indicus com a de suíno demonstrou diferenças em três resíduos de aminoácidos. Na comparação com ßFSH, registrou-se modificação na primeira base do codon que levou à alteração no resíduo do aminóacido 83, que, em Bos taurus indicus, é uma glicina, ao invés da serina presente em Bos taurus taurus. Confirmaram-se essa modificação e todas as outras identificadas na seqüência dos cDNA das cadeias αFSH e βFSH em outra clonagem. A modificação Ser para Gly na posição 83 foi a única que alterou a identidade do resíduo de aminoácido na comparação entre as subunidades beta do FSH de Bos taurus indicus e Bos taurus taurus. Contudo, ela não deve alterar significativamente as propriedades fisiológicas do FSH, uma vez que o resíduo de glicina encontrado nessa posição também é encontrado na cadeia βFSH suína. Trata-se, portanto, de uma modificação particular que distingue as cadeias βFSH de B. taurus taurus e B. taurus indicus.

     

    PALAVRAS-CHAVES: Bovino, clonagem, FSH, hormônio.

    The aim this work was to promove the cloning and analysis sequencing

  17. Quantitative analysis of N-glycans from human alfa-acid-glycoprotein using stable isotope labeling and zwitterionic hydrophilic interaction capillary liquid chromatography electrospray mass spectrometry as tool for pancreatic disease diagnosis

    Energy Technology Data Exchange (ETDEWEB)

    Giménez, Estela, E-mail: estelagimenez@ub.edu [Department of Analytical Chemistry, University of Barcelona, Diagonal 647, E-08028 Barcelona (Spain); Balmaña, Meritxell [Biochemistry and Molecular Biology Unit, Department of Biology, University of Girona, Campus Montilivi s/n, 17071 Girona (Spain); Figueras, Joan [Department of Surgery, Dr. Josep Trueta University Hospital, IdlBGi, 17007 Girona (Spain); Fort, Esther [Digestive Unit, Dr. Josep Trueta University Hospital, 17007 Girona (Spain); Bolós, Carme de [Gastroesophagic Cancer Research Group, Research Programme in Cancer, Hospital del Mar Medical Research Institute (IMIM), Dr. Aiguader, 88, 08003 Barcelona (Spain); Sanz-Nebot, Victòria [Department of Analytical Chemistry, University of Barcelona, Diagonal 647, E-08028 Barcelona (Spain); Peracaula, Rosa [Biochemistry and Molecular Biology Unit, Department of Biology, University of Girona, Campus Montilivi s/n, 17071 Girona (Spain); Rizzi, Andreas [Institute of Analytical Chemistry, University of Vienna, Währinger Straße 38, A-1090 Vienna (Austria)

    2015-03-25

    Highlights: • The method enables relative quantitation of hAGP glycans from pathological samples • Pancreatic cancer samples clearly showed an increase of hAGP fucosylated glycans. • Fucosylated glycans could be potential biomarkers for diagnosing pancreatic cancer. • The established method could be extremely useful to find novel glycoprotein biomarkers - Abstract: In this work we demonstrate the potential of glycan reductive isotope labeling (GRIL) using [{sup 12}C]- and [{sup 13}C]-coded aniline and zwitterionic hydrophilic interaction capillary liquid chromatography electrospray mass spectrometry (μZIC-HILIC-ESI-MS) for relative quantitation of glycosylation variants in selected glycoproteins present in samples from cancer patients. Human α{sub 1}-acid-glycoprotein (hAGP) is an acute phase serum glycoprotein whose glycosylation has been described to be altered in cancer and chronic inflammation. However, it is not clear yet whether some particular glycans in hAGP can be used as biomarker for differentiating between these two pathologies. In this work, hAGP was isolated by immunoaffinity chromatography (IAC) from serum samples of healthy individuals and from those suffering chronic pancreatitis and different stages of pancreatic cancer, respectively. After de-N-glycosylation, relative quantitation of the hAGP glycans was carried out using stable isotope labeling and μZIC-HILIC-ESI-MS analysis. First, protein denaturing conditions prior to PNGase F digestion were optimized to achieve quantitative digestion yields, and the reproducibility of the established methodology was evaluated with standard hAGP. Then, the proposed method was applied to the analysis of the clinical samples (control vs. pathological). Pancreatic cancer samples clearly showed an increase in the abundance of fucosylated glycans as the stage of the disease increases and this was unlike to samples from chronic pancreatitis. The results gained here indicate the mentioned glycan in h

  18. Quantitative analysis of N-glycans from human alfa-acid-glycoprotein using stable isotope labeling and zwitterionic hydrophilic interaction capillary liquid chromatography electrospray mass spectrometry as tool for pancreatic disease diagnosis

    International Nuclear Information System (INIS)

    Highlights: • The method enables relative quantitation of hAGP glycans from pathological samples • Pancreatic cancer samples clearly showed an increase of hAGP fucosylated glycans. • Fucosylated glycans could be potential biomarkers for diagnosing pancreatic cancer. • The established method could be extremely useful to find novel glycoprotein biomarkers - Abstract: In this work we demonstrate the potential of glycan reductive isotope labeling (GRIL) using [12C]- and [13C]-coded aniline and zwitterionic hydrophilic interaction capillary liquid chromatography electrospray mass spectrometry (μZIC-HILIC-ESI-MS) for relative quantitation of glycosylation variants in selected glycoproteins present in samples from cancer patients. Human α1-acid-glycoprotein (hAGP) is an acute phase serum glycoprotein whose glycosylation has been described to be altered in cancer and chronic inflammation. However, it is not clear yet whether some particular glycans in hAGP can be used as biomarker for differentiating between these two pathologies. In this work, hAGP was isolated by immunoaffinity chromatography (IAC) from serum samples of healthy individuals and from those suffering chronic pancreatitis and different stages of pancreatic cancer, respectively. After de-N-glycosylation, relative quantitation of the hAGP glycans was carried out using stable isotope labeling and μZIC-HILIC-ESI-MS analysis. First, protein denaturing conditions prior to PNGase F digestion were optimized to achieve quantitative digestion yields, and the reproducibility of the established methodology was evaluated with standard hAGP. Then, the proposed method was applied to the analysis of the clinical samples (control vs. pathological). Pancreatic cancer samples clearly showed an increase in the abundance of fucosylated glycans as the stage of the disease increases and this was unlike to samples from chronic pancreatitis. The results gained here indicate the mentioned glycan in hAGP as a candidate

  19. Efeito do treinamento físico como modulador positivo nas alterações no eixo neuroimunoendócrino em indivíduos com insuficiência cardíaca crônica: possível atuação do fator de necrose tumoral-alfa Efecto del entrenamiento físico como modulador positivo en las alteraciones en el eje neuroinmunoendócrino en indivíduos con insuficiência cardíaca crônica: posible actuación como factor de necrosis tumoral-alfa Effect of physical training as positive modulator on the alterations in the neuro-immune-endocrine axis in patients with chronic heart failure: possible role of the tumoral necrosis factor-alpha

    Directory of Open Access Journals (Sweden)

    Luís Fernando Bicudo Pereira Costa Rosa

    2005-08-01

    Full Text Available O exercício físico crônico ou o treinamento físico (TF tem sido largamente utilizado nos últimos anos com finalidade terapêutica e preventiva em uma série de condições fisiopatológicas, incluindo doenças cardiovasculares. Além dos benefícios cardiovasculares, o TF modula a expressão elevada de citocinas pró-inflamatórias, atuando sobre o eixo neuroimunoendócrino. Atualmente, o quadro de insuficiência cardíaca crônica (ICC tem sido reconsiderado como uma interação entre mecanismos hemodinâmicos, neurormonais, endócrinos e imunológicos. Esta resposta inflamatória anormal, incluindo a elevada expressão de citocinas pró-inflamatórias, tem sido proposta como responsável pela progressão e deterioração clínica na ICC. Neste quadro, a principal citocina envolvida no quadro fisiopatológico da ICC é o fator de necrose tumoral-alfa (TNF-alfa. Assim, o TF pode atuar no quadro de ICC de duas maneiras, melhorando o desempenho durante o exercício físico, bem como atenuando do quadro deletério de elevada concentração de citocinas pró-inflamatórias no sistema cardiovascular, podendo representar importante opção imunomodulatória e, desta forma, permitir melhora significativa no quadro clínico do paciente.El ejercicio físico o entrenamiento físico (TF viene siendo utilizado largamente en los ultimos años con finalidad terapéutica y preventiva en una serie de condiciones fisiopatológicas, incluyendo las afectaciones cardiovasculares. Además de los beneficios cardiovasculares, el TF modula la expresión elevada de las citocinas pro-inflamatorias, actuando sobre el eje neuroinmunoendócrino. Actualmente, el cuadro de insuficiencia cardíaca crônica (ICC viene siendo reconsiderado como una interación entre los mecanismos hemodinámicos, neuro-hormonales, endócrinos e imunológicos. Esta respuesta inflamatoria anormal, incluyendo la elevada expresión de citoquinas pro-inflamatorias, viene siendo propuesta como

  20. Sincronização do ciclo estral em vacas nelore com prostaglandi na F2-alfa análoga (ICI-80.996, Cloprostenol por via intramuscular

    Directory of Open Access Journals (Sweden)

    Vanderlei A. Benedito

    1980-11-01

    Full Text Available Estudo da eficiência da PGF2-a análoga (Cloprostenol, ICI-80.996 na sincronização do ciclo estral e a fertilidade no cio sincronizado. Pesquisa efetivada em rebanho da raça Nelore nas proximidades de Londrina, Paraná. No primeiro dia do experimento, após exame genital, foram separadas 132 vacas, na maioria com 7a 9 meses de paridas e as restantes, falhadas no ano anterior, formando, ao acaso, três grupos de 44. Dois grupos (1ell receberam duas injeções de 0,5 mg de Cloprostenol por via intramuscular com 11 dias de intervalo. Os animais do Grupo I foram inseminados em períodos pré-fixados (72 e 96 horas depois da segunda aplicação. O grupo II permaneceu com rufiões portadores de "chin-ball" e as inseminações aconteceram cerca de 12 horas após a identificação do cio. O terceiro grupo serviu como testemunha e o esquema de observação de cio e de inseminação foi o mesmo adotado no Grupo II, por um período de 21 dias. Os resultados obtidos foram os seguintes: Grupo I - sem qualquer preocupação com os sintomas de cio resultou 24 (54,54% vacas gestantes e das outras 20, houve um retorno sincronizado de 70%. Grupo II - entre o 2o. e 8o. dia, exibiram cio e foram inseminadas 42 (95,45% vacas e ficaram prenhes 15 (34,09%. Das 27 vacas que não enxertaram, 18 (66,66% repetiram ciclo normal, confirmando os achados do grupo anterior. Grupo III - o índice de cio e inseminação atingiu 43 (97,72% vacas, resultando 16 (36,36% gestações e as demais, voltaram para o regime de monta natural. O índice de fertilidade entre os grupos estudados não foi estatisticamente significativo (P> 0,05.The objective of this study is to determine the efficiency of PGF2-OL analogue (Cloprostenol, ICI-80.996 in the synchronization of the estrous cycle and the fertility level in Nelore cows from a farm adjacent to Londrina, Paraná State. On the first day of the trial, 132 cows were selected after genital examination, the majority of them, 7 to 9

  1. The Portuguese language version of social phobia and Anxiety Inventory: analysis of items and internal consistency in a Brazilian sample of 1,014 undergraduate students Versão para o português do Inventário de Fobia Social e Ansiedade: análise de itens e consistência interna numa amostra de 1.014 estudantes universitários brasileiros

    Directory of Open Access Journals (Sweden)

    Patrícia Picon

    2006-01-01

    Full Text Available OBJECTIVE: Theoretical and empirical analysis of items and internal consistency of the Portuguese-language version of Social Phobia and Anxiety Inventory (SPAI-Portuguese. METHODS: Social phobia experts conducted a 45-item content analysis of the SPAI-Portuguese administered to a sample of 1,014 university students. Item discrimination was evaluated by Student's t test; interitem, mean and item-to-total correlations, by Pearson coefficient; reliability was estimated by Cronbach's alpha. RESULTS: There was 100% agreement among experts concerning the 45 items. On the SPAI-Portuguese 43 items were discriminative (p OBJETIVO: Análise teórica e empírica dos itens e da consistência interna da versão em português do Social Phobia and Anxiety Inventory (SPAI-Português e subescalas. MÉTODOS: Peritos em fobia social conduziram análise de conteúdo dos 45 itens do SPAI-Português, administrado a 1.014 estudantes universitários. A discriminação dos itens foi avaliada por teste t de Student; correlações interitens, médias e item/total por coeficientes de Pearson; fidedignidade pelo alfa de Cronbach. RESULTADOS: Concordância plena entre os peritos para os 45 itens. SPAI-Português com 43 itens discriminativos (p < 0,05. Alguns itens, entre as subescalas, apresentaram coeficientes abaixo de 0,2. As médias das correlações interitens foram: 0,41 na subescala fobia social; 0,32 na subescala agorafobia; e 0,32 no SPAI-Português. As correlações item/total foram maiores do que 0,3 (p < 0,001. Alfas de Cronbach foram: 0,95 no SPAI-Português; 0,96 na subescala de fobia social; 0,85 na subescala de agorafobia. CONCLUSÃO: O conteúdo dos itens foi relacionado aos constructos subjacentes (agorafobia e fobia social, com discriminabilidade de 43 itens do SPAI-Português. As correlações médias interitens e alfas revelaram consistência interna de SPAI-Português e subescalas, além de multidimensionalidade das mesmas. Nenhum item foi suprimido

  2. Bases moleculares de alfa-talasemia en la Argentina

    Directory of Open Access Journals (Sweden)

    Karen G Scheps

    2015-04-01

    Full Text Available La α-talasemia, es uno de los desórdenes hereditarios más frecuentes mundialmente. Al presente, el diagnóstico molecular es la única herramienta que permite el diagnóstico certero. El propósito de este trabajo fue caracterizar las bases moleculares de estos síndromes en nuestro medio, y establecer relaciones genotipo-fenotipo. Mediante la complementación de distintas técnicas de biología molecular e hibridación fluorescente in situ (FISH, se logró poner en evidencia la presencia de mutaciones α-talasémicas en 145 de 184 (78.8% pacientes estudiados con perfil hematológico compatible con α-talasemia. Dentro de este grupo, las deleciones correspondieron al defecto genético más frecuente, prevaleciendo la mutación -α3.7 en genotipos heterocigotas y homocigotas. Asimismo, en pacientes con fenotipo α0 las deleciones prevalentes fueron -MED y -CAL/CAMP. Este estudio permitió también describir una deleción de la región sub-telomérica en un paciente con α-talasemia y retraso mental. En el 7.6% de los pacientes caracterizados clínicamente como posibles α-talasémicos (microcitosis con valores de Hb A2 inferiores al 3.5%, se hallaron mutaciones β-talasémicas en estado heterocigota. Se lograron establecer perfiles hematológicos asociados a los genotipos α+ y α0 para pacientes adultos y niños. Esperamos que este trabajo pueda servir como guía para reconocer posibles portadores α-talasémicos. También permite destacar el trabajo en conjunto de médicos hematólogos, el laboratorio (bioquímico y de biología molecular y de los médicos genetistas, con el fin de proporcionar adecuado consejo genético.

  3. Pathophysiology of hypophosphatasia and the potential role of asfotase alfa

    OpenAIRE

    Orimo, Hideo

    2016-01-01

    Hideo Orimo Division of Metabolism and Nutrition, Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan Abstract: Hypophosphatasia (HPP) is an inherited systemic bone disease that is characterized by bone hypomineralization. HPP is classified into six forms according to the age of onset and severity as perinatal (lethal), perinatal benign, infantile, childhood, adult, and odontohypophosphatasia. The causative gene of the disease is the ALPL gene that encodes ...

  4. Cancer blandt gigtpatienter behandlet med tumornekrosefaktor-alfa-haemmere

    DEFF Research Database (Denmark)

    Dreyer, Lene; Mellemkjaer, Lene; Hetland, Merete Lund

    2009-01-01

    associated with an increased cancer incidence. MATERIAL AND METHODS: A descriptive study of cancer cases among 3,688 patients with rheumatic diseases who received anti-TNF therapy was performed. The patients were identified in the nationwide database DANBIO and had a total number of treatment years of 6...... continue. Future studies must reveal whether anti-TNF therapy is associated with an increased cancer risk. Planned Danish studies will include relevant control groups and adjustment for confounders. Udgivelsesdato: 2009-Feb-9......,092 years. All cancer cases were identified and the cancer diagnoses were subsequently confirmed by medical record review. RESULTS: A total of 30 cancers in 28 of 3,688 patients were registered in the DANBIO after initiation of anti-TNF therapy in the period from October 2000 to June 2007. Among these six...

  5. YERALTISULARINDA TOPLAM ALFA VE BETA RADYOAKTİVİTESİ

    OpenAIRE

    Simge VAROL

    2011-01-01

    Two inseparable concepts are human and environment. Human is consistently communication within the environment. Water is life source of humans and it is the most beneficial component for lives. Ground waters include several chemical elements and minerals which control usage properties of water. These elements may create positive and negative effects in human body. Therefore, several researches are present related to physical, chemical, bacteriological and radioactivity properties of water. ...

  6. Desarrollo comunicación Alfa Arduino mediante Scada

    OpenAIRE

    Trallero Calvo, Jorge

    2015-01-01

    El siguiente proyecto pretende crear un sistema IoT (Internet of Things) girando en torno a Arduino, demostrando de este modo la facilidad de creación de proyectos y fiabilidad que nos aporta Arduino. Mediante una comunicación TCP/IP se procede a crear un sistema el cual es capaz de registrar valores a una cierta distancia mediante protocolos de procesos y comunicación (Telemedida) y de este modo monitorizar la temperatura en un punto determinado, sin estar limitado por la t...

  7. "Alfa-gerlz" rvutsja k vlasti / Ivan Lebedev

    Index Scriptorium Estoniae

    Lebedev, Ivan

    2007-01-01

    Naiste osast USA akadeemilistes ringkondades, majanduselus ja poliitikas. Esmakordselt võib saada USA presidendiks naispoliitik, Hillary Clintonit toetab üle 40 % demokraatidest. Vt. samas: Toimik: USA mõjukad naised

  8. HI Absorbers from Arecibo Legacy Fast ALFA Survey

    Science.gov (United States)

    Wu, Z. Z.; Haynes, M. P.; Giovanelli, R.; Zhu, M.; Chen, R. R.

    2015-03-01

    We present some preliminary results of an on-going study of the HI 21-cm absorption line based on the 40% data release of the Arecibo Legacy Fast Arecibo L-band Feed Array (ALFALFA) survey. Ten HI absorption candidates have been detected. Five of them are previously published in the literature, and the rest of them are new detections that need further confirmation. For those sources with no detected absorptions, we calculated the upper limits of their foreground HI column density N_{HI}. The statistical result of the N_{HI} distribution indicates that the ratio of average spin temperature to covering factor T_{s}/f for DLAs (the damped Lyα systems) might be larger than 500 K. Radio frequency interference (RFI) and standing waves are the main issues affecting the detection of HI absorption profiles. Our study can serve as the pathfinder for a large scale search of HI 21-cm absorption lines using the Five-Hundred-Meter Aperture Spherical Radio Telescope (FAST) which is an Arecibo-type radio telescope currently under construction in China. We discuss two types of observational studies of HI absorptions toward extragalactic sources using the FAST telescope.

  9. Alfa-fetoprotein secreting ovarian sex cord-stromal tumor

    Directory of Open Access Journals (Sweden)

    Kusum D Jashnani

    2013-01-01

    Full Text Available Ovarian sex cord-stromal tumors are relatively infrequent neoplasms that account for approximately 8% of all primary ovarian tumors. They are a heterogeneous group of neoplasms composed of cells derived from gonadal sex cords (granulosa and Sertoli cells, specialized gonadal stroma (theca and Leydig cells, and fibroblasts. They may show androgenic or estrogenic manifestations. We report such a tumor associated with markedly raised serum alpha-fetoprotein (AFP levels in a young female presenting with a mass and defeminising symptoms. Serum AFP levels returned to normal on removal of tumor.

  10. Recombinant Interferon Alfa-2b in Treating Patients With Melanoma

    Science.gov (United States)

    2016-05-17

    Stage IA Skin Melanoma; Stage IB Skin Melanoma; Stage IIA Skin Melanoma; Stage IIB Skin Melanoma; Stage IIC Skin Melanoma; Stage IIIA Skin Melanoma; Stage IIIB Skin Melanoma; Stage IIIC Skin Melanoma; Stage IV Skin Melanoma

  11. Optimizing Interferon Alfa Based Therapy for Chronic Hepatitis C

    NARCIS (Netherlands)

    R. Roomer (Robert)

    2011-01-01

    textabstractThe hepatitis C virus was first discovered in 1989 as the major cause of chronic non-A non-B hepatitis. The hepatitis C virus is a single stranded RNA virus that belongs to the family of flaviviruses. The primary target of the hepatitis C virus are hepatocytes where viral particles repli

  12. The pattern of immune cell infiltration in chromoblastomycosis: involvement of macrophage inflammatory protein-1 alpha/CCL3 and fungi persistence Padrão de infiltração de células do sistema imune na cromomicose: envolvimento de MIP-1 alfa da persistência fúngica

    OpenAIRE

    Vanuza Cristina Sá; Tarcília Aparecida Silva; Carmelia Matos Santiago Reis; Fernando Queiroz Cunha; Florêncio Figueiredo; Anamélia Lorenzetti Bocca

    2007-01-01

    Chromoblastomycosis (CR) is a subcutaneous chronic mycosis characterized by a granulomatous inflammatory response. However, little is known regarding the pattern of leukocyte subsets in CR and the pathways involved in their recruitment. The objective of this study was to assess the cellular subsets, chemokine, chemokine receptors and enzymes in CR. The inflammatory infiltrate was characterized by immunohistochemistry using antibodies against macrophages (CD68), Langerhans'cells (S100), lympho...

  13. Determinação sérica de haptoglobina, ceruloplasmina e alfa-glicoproteína ácida em cães com gastrenterite hemorrágica Determination of serum haptoglobin, ceruloplasmin and acid alpha-glycoprotein in dogs with haemorrhagic gastroenteritis

    Directory of Open Access Journals (Sweden)

    Márcia Mery Kogika

    2003-06-01

    Full Text Available As proteínas de fase aguda (PFA são proteínas plasmáticas, cujo estímulo à síntese ocorre de forma rápida e marcante em resposta à injúria tecidual. Estas proteínas permitem o diagnóstico de processos inflamatórios em animais com supressão ou depressão medular. Além disso, são úteis na monitorização da resolução tecidual de traumas ou inflamação e também na avaliação da resposta orgânica ao tratamento. Uma vez que a leucopenia é observada nos estádios iniciais da parvovirose canina, a dosagem das PFA pode permitir a avaliação do processo inflamatório sob estas condições. Considerando-se estas hipóteses, foram determinados os níveis séricos das PFA (haptoglobina, ceruloplasmina e alfa-glicoproteína ácida em 11 cães saudáveis e 11 cães leucopênicos com gastrenterite hemorrágica, com suspeita clínica de parvovirose canina. A avaliação estatística mostrou diferença significativa, com intervalo de confiabilidade de 99% (PAcute phase proteins (APP are serum proteins whose stimulus for the synthesis happens in a quick and intense manner in response to tissue injury. Those proteins allow the diagnosis of inflammatory process in animals with bone marrow depression and, also, they are useful in the follow up of tissue resolution of traumas or inflammation, as well as in the evaluation of the organic response of the treatment. As leukopenia is observed in the initial stage of the canine parvovirus infection, the dosage of APP can allow the evaluation of the inflammatory process under these conditions. According to these hypothesis, serum APP levels (haptoglobin, ceruloplasm and a-acid-glycoprotein in 11 healthy dogs and 11 leukopenic dogs with haemorrhagic gastroenteritis, clinically suspected of canine parvovirus infection, were measured. There was a significant difference, with confidence interval of 99% (P <0.01 for the haptoglobin (p <0.0064 and the acid alpha-glycoprotein (p <0.0042 and 95% (P <0.05 of

  14. Aproximación al uso del coeficiente alfa de Cronbach An Approach to the Use of Cronbach's Alfa

    OpenAIRE

    Heidi Celina Oviedo; Adalberto Campo-Arias

    2005-01-01

    Introducción: Las escalas se usan frecuentemente en la investigación y en la práctica clínica de la psiquiatría. No obstante, todas las escalas deben ser formalmente validadas antes, con el propósito de conocer de las propiedades psicométricas en una población específica. Descripción: Las escalas pueden ser validadas con el uso de un patrón de referencia o sin él; la segunda opción es la única forma de validar algunos instrumentos. Establecer la consistencia interna de una escala es una aprox...

  15. Th1 and Th2 immune responses related to pelvic endometriosis Resposta imunológica Th1 e Th2 relacionada à endometriose pélvica

    Directory of Open Access Journals (Sweden)

    Sergio Podgaec

    2010-01-01

    Full Text Available OBJECTIVE: This study analyzed the relationship between clinical characteristics of endometriosis and Th1/Th2 immune response patterns. METHODS: A prospective study was performed with 65 patients with endometriosis (Group A and 33 without the disease (Group B. Measurement of IL 2, 4 and 10, TNF-alpha and IFN-gamma was carried out in peripheral blood and peritoneal fluid. RESULTS: Serum TNF-alpha was higher in patients with endometriosis who had deep dyspareunia compared to controls (mean 4.5 pg/ml and 2.3 pg/ml, pOBJETIVO: Este estudo analisa a relação entre as características clínicas da endometriose e os padrões da resposta imune Th1/Th2. MÉTODOS: Estudo prospectivo realizado com 65 pacientes com endometriose (Grupo A e 33 pacientes sem a doença (Grupo B. Foram realizadas avaliação no fluido peritoneal e sangue periférico de IL 2, 4 e 10, TNF-alfa e IFN-gama. A significância foi estabelecida em p < 0,05. RESULTADOS: TNF-alfa encontrava-se elevado em pacientes com endometriose que apresentavam dispareunia de profundidade comparado com controle (média 4,5 pg/ml e 2,3 pg/ml, p< 0,05. Dentre essas pacientes (n=32, 65,5% apresentavam endometriose profunda. Pacientes com endometriose e infertilidade apresentavam concentrações maiores de IL-2 no fluido peritoneal quando comparadas com controle (média 5,9 pg/ml e 0,2 pg/ml, p< 0,05, sendo que neste grupo, 63,5% (n=14 apresentavam endometriose profunda. Foi observada também maior concentração de IL-10 nas pacientes que apresentavam endometriose ovariana quando comparadas às sem esse tipo de endometriose, assim como quando comparadas às pacientes do grupo controle (média 50pg/ml, 18,7pg/ml e 25,7pg/ml, p<0,05. CONCLUSÃO: Estes resultados sugerem que quando dados clínicos específicos associam-se a uma produção elevada de certas citocinas, ocorre um padrão de resposta Th1 que pode estar associado à endometriose profunda.

  16. Exclusion of linkage between hypokalemic periodic paralysis and a candidate region in 1q31-32 suggests genetic heterogeneity

    Energy Technology Data Exchange (ETDEWEB)

    Sillen, A.; Wadelius, C.; Gustabson, K.H. [University Hospital, Uppsala (Sweden)] [and others

    1994-09-01

    Familial hypokalemic periodic paralysis (HOKPP) is an autosomal dominant disease with attacks of paralysis of varying severity. The attacks occur at intervals of days to years in otherwise healthy people combined with hypokalemia during attacks. The paralysis attacks are precipitated by a number of different factors, like carbohydrate-rich meals, cold, exercise and mental stress. Recently linkage for HOKPP was shown for chromosome 1q31-32 and the disease was mapped between D1S413 and D1S249. The gene for the calcium channel alfa1-subunit (CACNL 1A3) maps to this interval and in two families no recombination was found between a polymorphism in the CACNL 1A3 gene and the disease. This gene is therefore considered to be a candidate for HOKPP. The analysis of a large Danish family excludes linkage to this region and to the CACNL 1A3 gene. In each direction from D1S413, 18.8 cM could be excluded and for D1S249, 14.9 cM. The present study clearly excludes the possibility that the gene causing HOKPP in a large Danish family is located in the region 1q31-32. This result shows that HOKPP is a heterogenous disease, with only one mapped gene so far.

  17. Investigação de fatores associados à asma de difícil controle Investigation of factors associated with difficult-to-control asthma

    Directory of Open Access Journals (Sweden)

    Ana Carla Sousa de Araujo

    2007-10-01

    Full Text Available OBJETIVO: Pesquisar a freqüência dos fatores associados à asma de difícil controle. MÉTODOS: Foram selecionados pacientes com diagnóstico de asma grave do ambulatório de asma do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto. Os pacientes foram classificados em dois grupos: asma grave controlada e asma grave de difícil controle. Após nova tentativa de otimização do tratamento para o grupo de difícil controle, foram aplicados questionário e investigação complementar de fatores associados, como exposição ambiental domiciliar e ocupacional, tabagismo, fatores sociais, rinossinusite, doença do refluxo gastroesofágico (DRGE, apnéia obstrutiva do sono, insuficiência cardíaca congestiva (ICC, embolia pulmonar, fibrose cística, disfunção de cordas vocais, deficiência de alfa-1 antitripsina e vasculite de Churg-Strauss. RESULTADOS: Foram selecionados 77 pacientes com asma grave, dos quais 47 apresentavam asma de difícil controle, sendo 68,1% do sexo feminino, idade média de 44,4 anos (±14,4 e volume expiratório forçado no primeiro segundo de 54,7% (±18,3%. Dos diagnósticos encontrados em associação à asma de difícil controle, o mais freqüente foi a pouca adesão ao tratamento (68%. Outros foram as más condições ambientais (34% e ocupacionais (17%, rinossinusite (57%, DRGE (49%, apnéia obstrutiva do sono (2%, ICC (2% e tabagismo (10%. Em todos os casos, pelo menos um desses fatores concomitantes foi diagnosticado. CONCLUSÕES: O fator mais freqüente associado à asma de difícil controle nos indivíduos estudados é a pouca adesão à medicação prescrita. A investigação de co-morbidades é imperativa na avaliação de pacientes com esta forma da doença.OBJECTIVE: To determine the prevalence of factors associated with difficult-to-control asthma. METHODS: Patients with severe asthma were selected from the outpatient asthma clinic of the Ribeirão Preto School of Medicine Hospital das Cl

  18. A meta-analysis of the relative doses of erythropoiesis-stimulating agents in patients undergoing dialysis

    OpenAIRE

    Bonafont, Xavier; Bock, Andreas; Carter, Dave; Brunkhorst, Reinhard; Carrera, Fernando; Iskedjian, Michael; Molemans, Bart; Dehmel, Bastian; Robbins, Sean

    2009-01-01

    Background. Erythropoiesis-stimulating agents (ESAs) such as epoetin alfa and beta, and darbepoetin alfa have improved the management of anaemia secondary to chronic kidney disease. Numerous studies have reported a dose reduction when patients receiving dialysis were converted from epoetin to darbepoetin alfa using the starting dose conversion of 200:1 as indicated on the prescribing label by the European Medicines Agency. The objective of this meta-analysis was to summarize the existing body...

  19. Epithelial-mesenchymal interactions and lung branching morphogenesis. Role of polyamines and transforming growth factor ß1

    Directory of Open Access Journals (Sweden)

    G Stabellini

    2009-12-01

    Full Text Available Lung branching morphogenesis is a result of epithelial-mesenchymal interactions, which are in turn dependent on extracellular matrix composition and cytokine regulation. Polyamines have recently been demonstrated as able to modify chick embryo skin differentiation. In this work we have examined the effects of putrescine and spermidine during chick embryo lung morphogenesis in organotypic cultures by morphological, histochemical and biochemical examination. To verify the role of polyamines, we used specific inhibitors, such as bis-cyclohexylammonium sulphate and alfa-difluoromethylornithine, and transforming growth factor ß1, an ornithine decarboxylase and polyamine stimulator. Our data show that lung morphogenesis is significantly altered following the induced mesenchymal glycosaminoglycan changes. The increase of mesenchymal glycosaminoglycans is correlated with a stimulation of lung development in the presence of polyamines, and with its inhibition when transforming growth factor ß1 is added to the culture medium. The morphometric data show a uniform increase of both the mesenchyme and epithelial branching with spermidine and putrescine stimulus, whereas the mesenchymal substance alone is significantly increased in apical-median lung sections with transforming growth factor ß1 and transforming growth factor ß1 + spermidine lung cultures. Transforming growth factor ß1 and transforming growth factor ß1 + spermidine confirm the blocking of epithelial branching formations and fibroblast activation, and show that polyamines are unable to prevent the blocking of epithelial cells due to the inhibitory effect of transforming growth factor ß1.

  20. 慢性HBV感染者外周血单个核细胞分泌干扰素α功能初探%The features of peripheral blood mononuclear cells secreting interferon-alfa in patients with chronic hepatitis B virus infection

    Institute of Scientific and Technical Information of China (English)

    程兆晶; 周宝桐; 张丽帆; 侍效春; 韩扬; 张月秋; 徐虹; 刘晓清

    2014-01-01

    目的:旨在检测慢性HBV感染者外周血单个核细胞(PBMC)分泌干扰素α(IFN-α)的情况,以探究其外周血浆细胞样树突状细胞(pDC)和单核细胞功能是否存在缺陷。方法前瞻性纳入2012年7月至2013年3月北京协和医院肝炎门诊就诊的慢性乙型肝炎(CHB)患者16例,同期选取与之年龄相匹配的HBV携带者16例和健康人18例。采集血样,分离PBMC,分别与含未甲基化的胞嘧啶鸟嘌呤二核苷酸序列的寡脱氧核苷酸2216(CPG ODN2216)、聚肌苷酸胞苷酸(poly I:C)刺激共培养,并用酶联免疫吸附试验(ELISA)测量其分泌IFN-α量。结果经ODN2216刺激后,CHB组患者PBMC分泌IFN-α量平均为31.20(7.33~44.04)pg/ml,HBV携带者组PBMC分泌IFN-α量平均为109.91(13.74~240.27)pg/ml,健康对照组PBMC分泌IFN-α量平均为107.95(48.59~227.33)。CHB患者组显著低于健康对照组,差异具有统计学意义(Z=-2.691,P=0.007)。CHB患者组显著低于HBV携带者组,差异具有统计学意义(Z=-2.206,P=0.027)。经poly(I:C)刺激后,三组PBMC分泌IFN-α量差异无统计学意义(F=0.628,P=0.427)。结论较HBV携带者和健康对照组,CHB患者体内PBMC中树突状细胞分泌IFN-α明显下降,而单核细胞分泌IFN-α量无显著性降低。%Objective To investigate the features of peripheral blood mononuclear cell (PBMC) secreting interferon-alfa (IFN-α) and evaluate the function of pDC and monocyte to produce IFN-αin patients with chronic hepatitis B virus infection. Methods The fresh blood samples were collected from 16 patients with chronic hepatitis B, 16 age matched patients with HBV carrier and 18 age matched healthy individuals as controls from July in 2012 to March in 2013 in Peking Union Medical College Hospital. PBMCs were isolated, respectively and then stimulated in vitro by CPG oligodeoxynucleotides (CPG ODN)2216 or polyinosinic

  1. Drug: D03918 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ke, and acute thrombotic diseases with high mortality and/or morbidity ATC code: B01AD10 recombina...0H3059N559O610S31 45658.6873 45688.0828 Antithrombotic; Profibrinalytic; Anti-inflammatory used in the treatment of sepsis, stro...nt human activated protein C [HSA:5624] [KO:K01344] Anatomical Therapeu...D03918 Drug Drotrecogin alfa (activated) (USAN); Drotrecogin alfa; Xigris (TN) C200...1A ANTITHROMBOTIC AGENTS B01AD Enzymes B01AD10 Drotrecogin alfa (activated) D03918 Drotrecogin alfa (activated) (USAN) CAS: 98530-76-8 PubChem: 47205907 DrugBank: DB00055 ...

  2. Níveis de proteína e de vitamina E para matrizes de frango de corte. 2. Efeito sobre a concentração de alfa-tocoferol na gema e nos tecidos e balanço de nitrogênio Protein and vitamin E levels for broiler breed hens. 2. Effects on yolk and tissue alpha-tocopherol concentration and nitrogen balance

    Directory of Open Access Journals (Sweden)

    S.L.T. Barreto

    1999-04-01

    Full Text Available Foram utilizadas 16 matrizes de frangos de corte com o objetivo de avaliar o efeito de dois níveis de proteína bruta (NPB, 14 e 16%, e dois níveis de vitamina E (NVE, 25 e 250mg/kg, na dieta sobre a concentração de alfa-tocoferol (AT na gema, no fígado, no soro sangüíneo e na excreta, e sobre a retenção de AT e de nitrogênio (N. O período experimental foi de 25 dias, sendo 15 dias para a adaptação das aves à dieta e 10 dias para a coleta de ovos e da excreta para análise de vitamina E (VE e N. O delineamento experimental foi o inteiramente ao acaso, formado por quatro tratamentos em esquema fatorial 2 × 2 (NVE × NPB, constituído cada um por quatro repetições, e cada unidade experimental representada por uma ave. Houve aumento linear (PSixteen broiler breed hens were used with the objective of evaluating the effects of supplementation of two crude protein (14 and 16% CP and two vitamin E levels (25 and 250mg VE/kg in the diet on the alpha-tocopherol (AT concentration in the egg yolk, liver, blood serum and feces, and on the AT and nitrogen (N retentions. The experiment lasted 25 days, in which 15 days were used for hens adaptation and 10 days for egg and fecal collection for AT and N analyses. The experimental design was a complete randomized design with a 2 × 2 factorial arrangement (CP × VE levels with four repetitions per treatment. The increasing of VE in the diet resulted in increase (P0.05. Thus, it could be concluded that the increasing of VE levels in the diet increased the AT concentrations in the egg yolk and body tissues, and decreased the AT and increased the N retention in broiler breed hens during the laying peak period.

  3. Genotype 1 hepatitis C virus envelope features that determine antiviral response assessed through optimal covariance networks.

    Directory of Open Access Journals (Sweden)

    John M Murray

    Full Text Available The poor response to the combined antiviral therapy of pegylated alfa-interferon and ribavarin for hepatitis C virus (HCV infection may be linked to mutations in the viral envelope gene E1E2 (env, which can result in escape from the immune response and higher efficacy of viral entry. Mutations that result in failure of therapy most likely require compensatory mutations to achieve sufficient change in envelope structure and function. Compensatory mutations were investigated by determining positions in the E1E2 gene where amino acids (aa covaried across groups of individuals. We assessed networks of covarying positions in E1E2 sequences that differentiated sustained virological response (SVR from non-response (NR in 43 genotype 1a (17 SVR, and 49 genotype 1b (25 SVR chronically HCV-infected individuals. Binary integer programming over covariance networks was used to extract aa combinations that differed between response groups. Genotype 1a E1E2 sequences exhibited higher degrees of covariance and clustered into 3 main groups while 1b sequences exhibited no clustering. Between 5 and 9 aa pairs were required to separate SVR from NR in each genotype. aa in hypervariable region 1 were 6 times more likely than chance to occur in the optimal networks. The pair 531-626 (EI appeared frequently in the optimal networks and was present in 6 of 9 NR in one of the 1a clusters. The most frequent pairs representing SVR were 431-481 (EE, 500-522 (QA in 1a, and 407-434 (AQ in 1b. Optimal networks based on covarying aa pairs in HCV envelope can indicate features that are associated with failure or success to antiviral therapy.

  4. Recent developments in the treatment of acute abdominal and facial attacks of hereditary angioedema: focus on human C1 esterase inhibitor.

    Science.gov (United States)

    Cardona, Lourdes Pastó; Bellfill, Ramon Lleonart; Caus, Joaquim Marcoval

    2010-01-01

    Hereditary angioedema (HAE) is a potentially fatal genetic disorder typified by a deficiency (type I) or dysfunction (type II) of the C1-inhibitor (C1-INH) and characterized by swelling of the extremities, face, trunk, abdominal viscera, and upper airway. Type III is normal estrogen-sensitive C1-INH HAE. Bradykinin, the main mediator of HAE, binds to endothelial B2 receptors, increasing vascular permeability and resulting in edema. HAE management includes short- and long-term prophylaxis. For treating acute episodes, C1-INH concentrate is recommended with regression of symptoms achieved in 30-90 min. Infusions of 500-1000 U have been used in Europe for years. Two plasma-derived C1-INH concentrates have been licensed recently in the United States: Berinert(®) for treating acute attacks and Cinryze(®) for prophylaxis in adolescent/adult patients. A recombinant C1-INH that is being considered for approval (conestat alfa) exhibited significant superiority versus placebo. Ecallantide (Kalbitor(®)) is a selective kallikrein inhibitor recently licensed in the United States for treating acute attacks in patients aged >16 years. It is administered in three 10-mg subcutaneous injections with the risk of anaphylactic reactions. Icatibant (Firazyr(®)) is a bradykinin B2 receptor competitor. It is administered subcutaneously as a 30-mg injection and approved in Europe but not in the United States. PMID:23776358

  5. Phase 1 safety, tolerability, and pharmacokinetic study of single ascending doses of XM17 (recombinant human follicle-stimulating hormone in downregulated healthy women

    Directory of Open Access Journals (Sweden)

    Lammerich A

    2015-07-01

    Full Text Available Andreas Lammerich, Peter Bias, Beate Gertz Merckle GmbH, Ulm, Germany Background: XM17 is a recombinant human follicle-stimulating hormone (follitropin alfa for stimulation of multifollicular development in women undergoing controlled ovarian hyperstimulation during assisted reproductive therapy and for treatment of anovulation. Manufactured using Chinese hamster ovary cells transfected with the human follicle-stimulating hormone gene, XM17 has an identical amino acid sequence to that of the human protein as well as to those of the other approved recombinant human follicle-stimulating hormone products. Glycosylation patterns may differ slightly between products. The objectives of this first-in-human study were to assess the safety, tolerability, pharmacokinetics, and dose-proportionality of single ascending subcutaneous doses of XM17 in healthy young female volunteers.Methods: Endogenous follicle-stimulating hormone was downregulated by implanting a 1-month depot of goserelin acetate 3.6 mg on day 0 in eligible subjects. On day 14 of the experimental period, subjects received one of four ascending doses of XM17. Blood sampling to obtain the pharmacokinetic profile of XM17 was done at frequent intervals until 168 hours post-dose.Results: Following downregulation of endogenous follicle-stimulating hormone to <4 IU/L, 40 subjects (of mean age 29±5.4 years received single subcutaneous doses of 37.5 (n=4, pilot group, 75, 150, or 300 IU (n=12 each of XM17. The mean serum concentration-time profiles of XM17 revealed dose-related increases in maximum concentration (Cmax within 24 hours followed by monoexponential decay for the three higher dose levels. Slopes estimated by linear regression for Cmax and AUC0–168h were ~1.0 (0.9052 IU/L and 1.0964 IU·h/L, respectively. For each IU of XM17 administered, Cmax and AUC0–168h rose by 0.032 IU/L and 2.60 IU·h/L, respectively. Geometric mean elimination half-life ranged from 54 to 90 hours. No antibodies

  6. 核苷(酸)类似物序贯干扰素治疗乙型肝炎病毒e抗原阳性慢性乙型肝炎患者的临床观察%Increase of hepatitis B surface antigen loss rate in hepatitis B e antigen positive chronic hepatitis B patients treated with nucleos (t) ide analogs and pegylated interferon alfa-2a sequential therapy

    Institute of Scientific and Technical Information of China (English)

    吴仲文; 章益民; 盛吉芳; 李兰娟

    2011-01-01

    Objective To investigate the changes of hepatitis B sarfaceantigen (HBsAg) titer in hepatitis B e antigen (HBeAg) positive chronic hepatitis B (CHB) patients treated with nucleos(t)ide analogs and pegylated interferon alfa-2a (PEG IFNα-2a) sequential therapy.Methods Among 6 HBeAg positive CHB patients,3 patients were treated with nucleos(t)ide analogs followed by PEG IFNα-2a for 48 weeks,3 patients were treated with nucleos(t)ide analog monotherapy.The serum HBsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc were detected using the time-resolved immunofluorometric assay and serum hepatitis B virus (HBV) DNA levels were determined by Taqman polymerase chain reaction (PCR) every 12 weeks.Results HBsAg loss were achieved in three patients after 48-week nucleos(t)ide analogs and PEG IFNα-2a sequential therapy.However,the HBsAg titers of another 3 patients varied from 100 IU/mL to 320 IU/mL.Conclusion In HBeAg positive CHB patients who obtain virologic response accompanied with HBsAg titer decreasing dramatically by nucleos(t) ide analog treatment,PEG IFNα-2a sequential treatment can increase HBsAg clearance rate.%目的 观察HBeAg阳性慢性乙型肝炎(CHB)患者在核苷(酸)类似物抗病毒治疗基础上序贯聚乙二醇干扰素α-2a(PEG IFNα-2a)治疗48周血清HBsAg的变化.方法 6例HBeAg阳性CHB患者中,3例采用核苷(酸)类似物序贯PEG IFNα-2a治疗48周,3例维持原核苷(酸)类似物治疗方案,每12周采用实时PCR定量检测HBV DNA,采用时间分辨免疫荧光分析法检测HBsAg、抗-HBs、HBeAg、抗-HBe及抗-HBc.结果 核苷(酸)类似物序贯PEG lFNα-2a治疗48周后,3例序贯治疗患者血清HBsAg均消失,而维持原核苷(酸)类似物治疗患者血清HBsAg效价为100~320 IU/mL.结论 对核苷(酸)类似物治疗产生较好应答反应且伴有血清HBsAg效价明显下降的HBeAg阳性CHB患者,在核苷(酸)类似物抗病毒治疗基础上序贯PEG IFNα-2a治疗48周能有效促进血清HBsAg下降,并出现血清HBsAg消失的现象.

  7. Leucograma e teores plasmáticos de proteínas de fase aguda de eqüinos portadores de abdômen agudo e submetidos à laparotomia Leukogram and plasma acute phase protein concentrations in horses with acute abdomen submitted to treatment by laparotomy

    OpenAIRE

    J.J. Fagliari; Silva, S.L.; P.C. Silva; G.T. Pereira

    2008-01-01

    Foram examinados 20 eqüinos adultos portadores de abdômen agudo e submetidos à laparotomia. Dez recuperaram-se sem intercorrência pós-operatória (G1) e 10 foram a óbito sete a 10 dias após a cirurgia, com sinais de choque séptico (G2). Avaliaram-se temperatura retal, freqüências cardíaca e respiratória, tempo de preenchimento capilar e teores plasmáticos das proteínas de fase aguda - fibrinogênio, ceruloplasmina, proteína C-reativa, antitripsina, haptoglobina e glicoproteína ácida -, antes e ...

  8. Effect of acupuncture on TNF-alpha, IL-1b and IL-10 concentrations in the peritoneal exudates of carrageenan-induced peritonitis in rats Efeito da acupuntura sobre a concentração de TNF-alfa, IL-1b e IL-10 no exsudato peritoneal de ratos com peritonite induzida por carragenina

    OpenAIRE

    Márcia Valéria Rizzo Scognamillo-Szabó; Gervásio Henrique Bechara; Fernando de Queiroz Cunha

    2005-01-01

    Acupuncture is an ancient and empirical therapeutic procedure known by its efficacy in the treatment of pain. However, the influence of acupuncture on inflammatory process is still poorly understood and additional research is needed. In this work, we investigated the mechanism of action of manual acupuncture on the inhibition of neutrophil migration to the peritoneal cavity induced by the inflammatory stimulus carrageenan in Wistar rats. Previous results from our laboratory showed that this a...

  9. Drug: D00172 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D00172 Drug Eptacog alfa (activated) (genetical recombination) (JAN); Eptacog alfa ...a preparations D00172 Eptacog alfa (activated) (genetical recombination) (JAN); Eptacog alfa (INN) Anatomica...oagulation factor VII D00172 Eptacog alfa (activated) (genetical recombination) (JAN); Eptacog alfa (INN) B0...2BD08 Eptacog alfa (activated) D00172 Eptacog alfa (activated) (genetical recombi

  10. Studio 1:1

    Index Scriptorium Estoniae

    2011-01-01

    Projekteerimis- ja disainistuudiost STUDIO 1:1 (Eindhoven), mis tegeleb avaliku ruumi loomise ja selle kasutamise kujundamisega. Projektidest "Linnulinn - linn lindude segakogukonna jaoks" ja ICT kogemuskeskus "De Verdieping"

  11. WOOD 1:1

    DEFF Research Database (Denmark)

    2013-01-01

    Report from 1:1 workshop at Virserum Konsthall in May 2013 with a team of Master's students from Royal Academy of Fine Arts, School of Architecture, Dpt. 3.......Report from 1:1 workshop at Virserum Konsthall in May 2013 with a team of Master's students from Royal Academy of Fine Arts, School of Architecture, Dpt. 3....

  12. 聚乙二醇干扰素联合利巴韦林治疗慢性丙型肝炎疗效观察%EFFICACY OF COMBINATION THERAPY WITH PEGYLATED-INTERFERON ALFA-2A PLUS RIBAVIRIN IN CHRONIC HEPATITIS C PATIENTS

    Institute of Scientific and Technical Information of China (English)

    秦金环

    2014-01-01

    目的评价聚乙二醇干扰素联合利巴韦林治疗慢性丙型肝炎的临床疗效.方法聚乙二醇干扰素α-2a(派罗欣)联合利巴韦林(800~1 200mg/d)治疗58例慢性丙型肝炎患者,疗程48周,分别于治疗4、12、24和48周及治疗结束后24周评价疗效,并观察药物副作用.结果 RVR:36例(62.1%)EVR:18例(31.0%),SLVR:3例(5.2%),ETVR 54例(93.1%),SVR:52例(89.7%);HCV RNA高水平组和低水平组之间持续应答率分别为66.7%和50.0%,差异有统计学意义(P<0.05).结论 在慢性丙型肝炎患者的治疗中SVR 54(93.1%),HCV RNA低水平组的疗效优于高水平组.

  13. Uso de dexmedetomidina em anestesiologia

    OpenAIRE

    Villela Nivaldo Ribeiro; Nascimento Júnior Paulo do

    2003-01-01

    JUSTIFICATIVA E OBJETIVOS: A dexmedetomidina é um novo agonista alfa2-adrenérgico que apresenta relação de seletividade entre os receptores alfa2:alfa1 de 1600:1, com importante ação sedativa e analgésica, bom controle hemodinâmico frente ao estresse e que pode produzir, por si só, anestesia. Este agente tem sido empregado para promover analgesia e sedação no período pós-operatório e nas unidades de tratamento intensivo. Devido à suas propriedades, recentemente, a dexmedetomidina passou a ser...

  14. Eficácia analgésica da dexmedetomidina comparada ao sufentanil em cirurgias intraperitoneais: estudo comparativo Eficacia analgésica de la dexmedetomidina comparada al sufentanil en cirugías intraperitoneales: estudio comparativo Analgesic efficacy of dexmedetomidine as compared to sufentanil in intraperitoneal surgeries: comparative study

    OpenAIRE

    Marco Aurélio Marangoni; Yara Marcondes Machado Castiglia; Tiago Pechutti Medeiros

    2005-01-01

    JUSTIFICATIVA E OBJETIVOS: A dexmedetomidina, agonista alfa2-adrenérgico com especificidade alfa1:alfa2 1:1620, não determina depressão respiratória, sendo utilizada no intra-operatório como sedativo e analgésico. Esse fármaco tem sido empregado com os opióides em anestesia de procedimentos com elevado estímulo doloroso, como os abdominais intraperitoneais, não havendo referências sobre seu uso como analgésico único. Comparou-se a dexmedetomidina ao sufentanil em procedimentos intraperitoneai...

  15. Estudo comparativo do uso isolado de plasma rico em plaquetas e combinado com cimento de alfa-fosfato tricálcico no reparo ósseo em ratos Comparative study on use of platelet-rich plasma alone and in combination with alpha-tricalcium phosphate cement for bone repair in rats

    Directory of Open Access Journals (Sweden)

    Alessandra Deise Sebben

    2012-01-01

    Full Text Available OBJETIVOS: Avaliar o efeito do cimento α-TCP combinado com PRP sobre a osteogênese, comparando os resultados com PRP utilizado isoladamente. MÉTODOS: Foi confeccionado defeito bilateral no fêmur de ratos e preenchido com um dos dois tipos de tratamentos (PRP ou α-TCP+PRP, sendo avaliado em quatro e oito semanas. As imagens radiográficas forneceram valores da área da lesão, e a histologia (coloração Picrosirius indicou a área de neoformação óssea. RESULTADOS: As médias referentes à área de lesão do grupo α-TCP+PRP (2,64mm² ± 2,07 e 1,91mm² ± 0,93; quatro e oito semanas, respectivamente demonstraram numericamente melhores resultados, porém não significativos (p > 0,05, em comparação com aqueles observados no grupo PRP (5,59mm² ± 2,69 e 3,23mm² ± 1,46; quatro e oito semanas, respectivamente. As médias de neoformação óssea foram de 62,7% ± 12,1% e 79,01% ± 6,25 no grupo PRP, e 73,3% ± 12,7 e 85,86% ± 10,45 no grupo α-TCP+PRP, em quatro e oito semanas, respectivamente (p > 0,05. CONCLUSÃO: Os dados deste estudo sugerem que o tratamento com cimento α-TCP combinado com PRP não demonstra diferença significativa quando comparado ao PRP isolado. Entretanto, há um possível efeito precoce sobre a regeneração óssea quando os dois biomateriais são aplicados em conjunto.OBJECTIVES: To evaluate the effect of alpha-tricalcium phosphate (α-TCP cement combined with platelet-rich plasma (PRP on osteogenesis, and to compare the results with use of PRP alone. METHODS: A bilateral defect was produced in rat femurs and was filled with one of two types of treatments (PRP or α-TCP + PRP. The outcomes were evaluated after four and eight weeks. Radiographic images provided values for the lesion area, and histology (picrosirius staining indicated the area of ​​new bone formation. RESULTS: The means relating to the lesion area of the α-TCP + PRP group (2.64 ± 2.07 and 1.91 ± 0.93 mm², after four and eight weeks

  16. High flow air sampling for determination of alpha long half-life emitters: area monitoring of a radioactive material disposal; Amostragem de ar de alto fluxo para determinacao de alfa emissores de meia vida longa: monitoracao de area em um deposito de material radioativo

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, W.S. [Industrias Nucleares do Brasil (SR/FCN/INB), Itatiaia, RJ (Brazil). Servico de Radioprotecao. Fabrica do Combustivel Nuclear; Kelecom, A. [Universidade Federal Fluminense (LARARA-PLS/GETA/UFF), Niteroi, RJ (Brazil). Lab. de Radiobiologia e Radiometria Pedro Lopes dos Santos. Grupo de Estudos em Temas Ambientais

    2015-07-01

    The Ore Treatment Unit (UTM) is a mine and closed uranium plant, located in Caldas, Minas Gerais, Brazil. It has a radioactive material disposal composed primarily of pie II and mesothorium. It is stored in six sheds designated C-01, C-02, C-05, C-06, C-07 and C-09. This study aims to present the high flow area monitoring program and results obtained in 2009. The threshold derived from concentration in the air was 0.25 Bq m{sup -3}. The average of the activity concentrations in 2009 were: for C-01 1.17 Bq m{sup -3}; C-02 0.006 Bq m{sup -3}; C-05 1.98 Bq m{sup -3}; C-06 2.14 Bq m{sup -3}; C-07 0.34 Bq m{sup -3} and C-09 0,025 Bq m{sup -3}. Such values indicate that the control stay is an important factor in occupational workers' control, as well as the use of EPI's and behavioral care, besides radioprotection training to allow the access to the areas. No worker, supervisor or visitor reached the limit research.

  17. Alpha and gamma spectrometry applied to the study of U and Th series radioactive disequilibrium in the phosphates from Abrolhos Archipelago (Brazil offshore); Aplicacao dos metodos de espectrometria alfa e gama ao estudo do desequilibrio radioativo das series do U e Th nos fosfatos do Arquipelago de Abrolhos

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Paulo Sergio Cardoso da; Marques, Leila Soares [Sao Paulo Univ., SP (Brazil). Inst. Astronomico e Geofisico

    1999-11-01

    This paper presents the results of uranium and thorium measurements made in aluminium and iron phosphates that occur in the Abrolhos Archipelago (offshore Brazil) by instrumental neutron activation analysis. The U concentrations were also indirectly determined from the gamma-ray activity of {sup 234} Th, {sup 214} Pb and {sup 214} Bi, while those of Th were obtained from the {sup 228} Ca, {sup 212} Pb, {sup 212} Bi and {sup 208} T1 gamma-ray emitters. In both cases it was assumed that {sup 238} U and {sup 232} Th series are in radioactive secular equilibrium. The comparison between the results obtained by neutron activation and gamma-ray spectrometry show radioactive secular equilibrium in the analysed samples. (author) 12 refs., 5 figs.; e-mail: pscarsil e leila at iag.usp.br

  18. 替比夫定治疗HBeAg阳性慢性乙肝部分应答患者的疗效观察%Effects of telbivudine treatment on HBeAg-positive chronic hepatitis B patient with partial responses for pegylated interferon alfa-2a therapy

    Institute of Scientific and Technical Information of China (English)

    黄胜; 桂亚萍; 伍沁芳; 黄伟; 余庆新; 潘长焰; 张江海

    2014-01-01

    目的 探讨聚乙二醇干扰素治疗HBeAg阳性慢性乙肝52周部分应答患者换用替比夫定的疗效及安全性.方法 对聚乙二醇干扰素治疗HBeAg阳性慢性乙肝52周时部分应答患者改用替比夫定(600 mg、1/d)继续治疗,分别于0、12、24、36及48周检测HBeAg、HBsAg、HBV DNA、血清谷丙转氨酶(ALT)及肌酸激酶水平.结果 基线ALT水平影响干扰素抗病毒疗效(P=0.026).换用替比夫定治疗后,血清HBV DNA在24周时全部阴转,HBeAg转换率由12周时的31.3%,增至48周时的62.5%,HBsAg水平进一步降低,治疗过程中无明显不良反应.结论 干扰素部分应答患者换用替比夫定能使HBV DNA及HBsAg水平进一步降低,提高HBeAg血清学转换率,安全性好,值得借鉴.%Objective To investigate the efficacy and safety of switched to telbivudine treatment for 48 weeks in HBeAg-positive chronic hepatitis B patient with poor responses after 52-week pegylated interferon alpha-2a therapy.Methods Patients with poor responses after 52-week pegylated interferon alpha-2a therapy were switched to telbivudine treatment (600 mg,1/day).At 0 (baseline),12,24,36 and 48 weeks,the levels of HBeAg,HBsAg,HBV DNA,serum ALT and CK were measured.Results Baseline ALT levels affect the antiviral efficacy of interferon (P=0.026).After switched to telbivudine treatment,serum levels HBV DNA of all patients were undetectable at 24 weeks; HBeAg seroconversion rate was increased from 31.3% at 12 weeks to 62.5% at 48 weeks.The mean HBsAg level was also reduced significantly and no significant side effect occurred over telbivudine treatment.Conclusion Telbivudine treatment can further reduce HBV DNA and HBsAg levels and increase the HBeAg seroconversion rate in HBeAg-positive chronic hepatitis B patient with poor responses for pegylated interferon alpha-2a therapy.

  19. 艾司西酞普兰干预干扰素治疗慢性丙型病毒性肝炎引发的精神症状%Escitalopram for intervention of psychiatric adverse events during peginterferon-alfa-2a and ribavirin treatment for chronic hepatitis C

    Institute of Scientific and Technical Information of China (English)

    齐明华; 周斌; 苏梅蕾; 潘集阳; 张浩; 张艳茹; 张健; 李威; 王俊洁

    2013-01-01

    Objective To evaluate the risk factors of psychiatric adverse events associated with PEG interferon and ribavirin treatment for chronic hepatitis C and assess the efficacy of escitalopram intervention for these adverse effects.Methods Fifty-nine patients with chronic hepatitis C undergoing interferon-based treatment for 12 weeks were assessed for major depression using DSM-Ⅳ and SCL-90,and the patients identified to have major depression received escitalopram treatment for intervention.SCL-90 was used to assess the psychological condition of the patients at the forth and eighth weeks of escitalopram treatment.Results A male gender,1b genotype,and intravenous infection are all risk factors of major depression.The morbidity rate of interferon-based depression was 32.2% with rates of hostility,anxiety,depression and sensitivity of 19.7%,9.2%,and 5.26%,respectively.The total score of SCL-90 and scores for hostility,anxiety,depression and sensitivity all significantly declined after escitalopram treatment in the 19 patients with major depression.Conclusions Psychological symptoms are common in HCV patients receiving interferon treatment,for whom regular psychological assessment is essential especially for those patients with drug abuse.Prompt use of escitalopram is recommended for effective control of major depression or other psychological symptoms in these patients.%目的 旨在评估引起这些精神心理症状的易感因素并分析艾司西酞普兰干预这些症状的效果.方法 59例慢性丙型病毒性肝炎患者,经聚乙二醇干扰素联合利巴韦林的抗病毒治疗,12周时由精神科医师使用DSM-Ⅳ标准进行评估,并指导患者使用SCL-90量表自评.对于符合重度抑郁标准者进行艾司西酞普兰干预治疗,在用药后4周,8周时再次行SCL-90测评.结果 中重度抑郁的易感因素有:男性,1b基因型,感染途径为静脉药瘾.干扰素相关中重度抑郁的发病率为32.2%.其他精神症状,

  20. Clinical Study on Patients with YMDD Mutation Chronic Hepatitis B Aftert the Treatment with Adefovir Dipivoxil in Combined with Interferon Alfa-2b%阿德福韦酯与干扰素联合治疗拉米夫定耐药慢性乙型肝炎临床研究

    Institute of Scientific and Technical Information of China (English)

    高栋; 李月波; 贾德兴; 辛广芹; 马桂芹

    2009-01-01

    目的 观察阿德福韦酯(ADV)联合干扰素α-2b治疗拉米夫定耐药的慢性乙型肝炎(CHB)的生化学改变、病毒学应答、持久性应答.方法 将97例患者随机分为两组.阿德福韦酯(贺维力)联合干扰素α-2b(安福隆)治疗组49例,阿德福韦酯10mg/d,疗程72周,干扰素α-2b 300万u,隔日1次肌肉注射,疗程48周;阿德福韦酯(贺维力)单药治疗组48例,阿德福韦酯10mg/d,疗程72周.治疗第48周、72周、停药后随访至96周的肝功能、HBV DNA定量作为评价指标.结果 联合组治疗48周、72周及停药后随访至96周时的HBV DNA阴转率分别为54.3%(P<0.05),63%(P<0.05),60.9%(P<0.01),肝功能好转.结论 阿德福韦酯联合干扰素α-2b治疗YMDD变异株HBeAg阴性慢性乙型肝炎,其生化学改变、病毒学应答率、持久应答率均优于单用阿德福韦酯.

  1. 78 FR 57166 - Antiviral Drugs Advisory Committee; Notice of Meeting

    Science.gov (United States)

    2013-09-17

    ... the treatment of chronic hepatitis C genotype 1 infection, in combination with peginterferon alfa and... disease (including cirrhosis) who are treatment- na ve or who have failed previous interferon...

  2. Drug: D05201 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D05201 Drug Nonacog alfa (genetical recombination) (JAN); Nonacog alfa (USAN/INN); ...3 Plasma preparations D05201 Nonacog alfa (genetical recombination) (JAN); Nonacog alfa (USAN/INN) Anatomica...onacog alfa D05201 Nonacog alfa (genetical recombination) (JAN); Nonacog alfa (USAN/INN) CAS: 181054-95-5 113478-33-4 PubChem: 47206920 ...

  3. Buffalo milk and cheese from animal to human nutrition. Part 1: the unsaponifiable fraction

    Directory of Open Access Journals (Sweden)

    M. Mattera

    2010-02-01

    Full Text Available With the aim to evaluate nutritional quality, samples of milk and Mozzarella di Bufala Campana PDO have been studied analyzing cholesterol, alfa tocopherol and trans retinol, functional components of the unsaponifiable fraction. Dairy products of experimental and commercial origin have been sampled. A large variability has been observed among the products but, regarding the nutrients studied, commercial and experimental farms produce mozzarella cheeses of comparable nutritional quality.

  4. Measurement of very low alpha activity in water; Medida de muy baja actividad alfa en aguas

    Energy Technology Data Exchange (ETDEWEB)

    Crespo, M. T.; Acena, M. L.

    1987-07-01

    Measurement of very low levels of alpha-emitting nuclides in water needs substantial improvements. A system based on the adsorbing properties manganese dioxide eliminates the need for transporting very large volumes of water and increases the sensitivity of the measurement. (Author) 21 refs.

  5. Mikrobiální biodegradace 17alfa-ethinylestradiolu a bisfenolu A

    Czech Academy of Sciences Publication Activity Database

    Křesinová, Zdena; Svobodová, Kateřina; Moeder, M.; Cajthaml, Tomáš

    Praha : PCHE-PetroChemEng, 2009, s. 3624-3627. ISBN 978-80-02-02108-7. [Odpadové fórum. Milovy (CZ), 22.04.2009-24.04.2009] R&D Projects: GA MŠk LC06066 Institutional research plan: CEZ:AV0Z50200510 Keywords : ligninolytic fungi * biobiodegradation * endocrine disrupting compounds Subject RIV: EE - Microbiology, Virology

  6. Pengaruh Insentif dan Jaminan Kesehatan TerhadapMotivasi Kerja Karyawan padaPT. Alfa Scorpii Medan

    OpenAIRE

    Tobing, Hanna Diaji

    2016-01-01

    Buying interest is an important step that must be passed consumers before deciding to conduct the purchase of a product. Buying interest alone can identified as follows: buying interest where consumers tend to carry out the activities of purchasing, buying interest where consumers tend to give advice to others to buy the product, buying interest where consumers choose a product become the primary choice, which is where the buying interest consumers seek to know information about a product. ...

  7. The possible effects of alfa and beta radiolysis on the matrix dissolution of spent nuclear fuel

    International Nuclear Information System (INIS)

    The effects of oxidants on the retainment of actinides in a nuclear repository have been modelled by using an equilirium procedure. The oxidants are formed as a result of α- and #betta#-radiolysis when spent nuclear fuel is exposed to ground water. From an equilibrium point of view, the strongest reductants in the system (Zr, Pb and Cu) are expected to be oxidized first, leaving the actinoids in the oxidation states they have in the fuel matrix. This is expected to result in a negligible mobilization of the actinoids due to the very low solubility of the MO2 oxides. However, the formation of protective layers of oxides will most likely decrease the effectiveness of the metallic reducing agents. This will lead to an increased oxidation of the spent fuel which results in an increased actinoid mobilization. The results of the equilibrium calculations show that the oxidation of the fuel matrix results in the formation of UO2(OH)2(s) and to the formation of the soluble complex UO2(CO3)34. The transport of uranium is limited by the total concentration of carbonate in the aqueous phase. Neptunium may be quantitatvely solubilized as various Np(V) species and transported by ground water from the repository. Plutonium is retained at the repository site as insoluble PuO2. Only very small amounts are transported by ground water. The mobile actinoids may be reprecipitated when they encounter reducing conditions along the flow path. The conditions for repricipitation for typical ground water compositions have been modelled by using solubility - pe diagrams. (Authors)

  8. Structural Evolution of Human Recombinant alfaB-Crystallin under UV Irradiation

    DEFF Research Database (Denmark)

    Sugiyama, Masaaki; Fujii, Noriko; Morimoto, Yukio;

    2008-01-01

    External stresses cause certain proteins to lose their regular structure and aggregate. In order to clarify this abnormal aggregation process, a structural evolution of human recombinant aB-crystallin under UV irradiation was observed with in situ small-angle neutron scattering. The abnormal...

  9. Managing infertility with the follitropin alfa prefilled pen injector – patient considerations

    OpenAIRE

    Bühler K

    2015-01-01

    Klaus Bühler Centre for Gynaecological Endocrinology and Reproductive Medicine, Stuttgart, Germany Abstract: Gonadotropin treatment has been used in fertility treatment since the 1930s. First, preparations coming from animals were injected, then, gonadotropins prepared from the pituitary glands of human cadavers. A great step was achieved with the introduction of human menopausal gonadotropin extracted from the urine of postmenopausal women. When cases of Creutzfeld-Jacob disease w...

  10. Induction, purification and characterization of alfa-N-acetylgalactosaminidase from Aspergillus Niger

    Czech Academy of Sciences Publication Activity Database

    Weignerová, Lenka; Filipi, Tomáš; Manglová, Dana; Křen, Vladimír

    2008-01-01

    Roč. 79, č. 5 (2008), s. 769-774. ISSN 0175-7598 R&D Projects: GA MŠk(CZ) LC06010; GA ČR GA203/05/0172 Institutional research plan: CEZ:AV0Z50200510 Keywords : aspergillus niger * glycosidase library Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 2.569, year: 2008

  11. ISTALLAZIONE DI UNA STAZIONE PER LA RIVELAZIONE CONTINUA RADON MEDIANTE SPETTROMETRIA ALFA NELLA SOLFATARA DI POZZUOLI

    OpenAIRE

    De Cesare, W.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione OV, Napoli, Italia; Scarpato, G.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione OV, Napoli, Italia; Buonocunto, C.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione OV, Napoli, Italia; Caputo, A.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione OV, Napoli, Italia; Capello, M.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione OV, Napoli, Italia; Avino, R.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione OV, Napoli, Italia; Roca, V.; Istituto Nazionale di Fisica Nucleare (Sezione di Napoli); De Cicco, F.; Istituto Nazionale di Fisica Nucleare (Sezione di Napoli); Pugliese, M. G.; Istituto Nazionale di Fisica Nucleare (Sezione di Napoli); Sabbarese, C.; Seconda Università degli Studi di Napoli (Dipartimento di Scienze Ambientali); Giudicepietro, F.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione OV, Napoli, Italia

    2011-01-01

    La realizzazione della stazione descritta nel presente rapporto è frutto di una collaborazione tra ricercatori della Sezione di Napoli dell’ Istituto Nazionale di Fisica Nucleare (INFN) e ricercatori dell’Osservatorio Vesuviano (OV), Sezione di Napoli dell’Istituto Nazionale di Geofisica e Vulcanologia (INGV), e costituisce un’attività sperimentale per il monitoraggio dell’attività vulcanica dei Campi Flegrei.

  12. Vicisitudes de la función alfa en la adolescencia y el climaterio

    OpenAIRE

    Campo, Claudia Inés; Flores, Graciela Elena; Marchisio, Silvina Alejandra

    2009-01-01

    Este artículo se deriva del Proyecto de Investigación Nº 4-2-0303, 22H/635: “El proceso de simbolización de las experiencias emocionales. Una indagación psicoanalítica de sus perturbaciones en el crecimiento mental”, aprobado por la Secretaría de Ciencia y Técnica de la Facultad de Ciencias Humanas de la Universidad Nacional de San Luis. Uno de los objetivos generales de la Investigación es explorar las características del equipo mental del que disponen los sujetos que atraviesan determinadas...

  13. Medida de la concentración de actividad Alfa/Beta grueso, en agua potable

    OpenAIRE

    Zutta Villate, Julián Mateo

    2014-01-01

    Introducción: La calidad del agua de consumo humano es de creciente interés en el mundo; para que esta pueda ser considerada como potable, debe ajustarse a ciertos niveles biológicos, químicos, físicos y radiológicos. Siguiendo recomendaciones de organismos internacionales de salud o, a estándares emitidos por cada país, establecidos según sus condiciones tanto ambientales, como socioeconómicas. La Organización Mundial de la Salud (OMS), en sus guías de potabilización del agua de consu...

  14. Alfa-tocopherol and beta-carotene in roughages and milk in organic dairy herds

    OpenAIRE

    Mogensen, Lisbeth; Kristensen, Troels; Søegaard, Karen; Jensen, Søren K; Sehested, Jakob

    2011-01-01

    The aim of the present study was to increase our knowledge about the vitamin content in roughage at harvest and during storage on organic commercial farms and to analyze the vitamin content in the milk. Roughages produced at the five organic dairy farms were monitored from harvest when the silage was laid in the silo and until the roughage was fed during the winter. The roughages had average α-tocopherol and β-carotene contents during the feeding period of, respectively, 30 mg and 21 mg per k...

  15. Alpha particles spectrometer with photodiode PIN; Espectrometro de particulas alfa con fotodiodo PIN

    Energy Technology Data Exchange (ETDEWEB)

    Chacon R, A.; Hernandez V, R.; Hernandez D, V. M.; Vega C, H. R. [Universidad Autonoma de Zacatecas, Unidades Academicas de Estudios Nucleares e Ingenieria Electrica, Calle Cipres No. 10, Fracc. La Penuela, 09869 Zacatecas (Mexico); Ramirez G, J. [Instituto Nacional de Estadistica Geografia e Informatica, Direccion General de Innovacion y Tecnologia de Informacion, Av. Heroes de Nacozari Sur 2301, Fracc. Jardines del Parque, 20276 Aguascalientes (Mexico)], e-mail: achruiz@hotmail.com

    2009-10-15

    The radiation propagates in form of electromagnetic waves or corpuscular radiation; if the radiation energy causes ionization in environment that crosses it is considered ionizing radiation. To detect radiation several detectors types are used, if the radiation are alpha particles are used detectors proportional type or trace elements. In this work the design results, construction and tests of an alpha particles spectrometer are presented, which was designed starting from a photodiode PIN type. The system design was simulated with a code for electronic circuits. With results of simulation phase was constructed the electronic phase that is coupled to a multichannel analyzer. The resulting electronic is evaluated analyzing the electronic circuit performance before an alphas triple source and alpha radiation that produce two smoke detectors of domestic use. On the tests phase we find that the system allows obtain, in a multichannel, the pulses height spectrum, with which we calibrate the system. (Author)

  16. How Clearly Written are Children's Textbooks? or, of Bladderworts and Alfa. Reading Education Report No. 16.

    Science.gov (United States)

    Anderson, Thomas H.; And Others

    Two representative samples of expository prose from sixth grade textbooks (one in science and one in social studies) were analyzed for clarity of explanation. Four text criteria were applied to the analyses: structure, unity, coherence, and audience appropriateness. The results of the analysis suggested that many children's textbooks are not…

  17. Dinamica numerica di microsonde verso Alfa Centauri con impulsi LASER su vele spaziali

    Science.gov (United States)

    Sigismondi, Costantino

    2016-05-01

    Starshot project aims to reach alpha Centauri at 4.4 light years in 24 years, by accelerating to velocity 0.2 c with collimated LASER light pressure for 1000s several grams-scale microprobes with space sails. The energetics and dynamical equations are solved numerically on a worksheet and discussed.

  18. Alfa-tocopherol and beta-carotene in roughages and milk in organic dairy herds

    DEFF Research Database (Denmark)

    Mogensen, Lisbeth; Kristensen, Troels; Søegaard, Karen;

    2012-01-01

    The aim of the present on-farm study was to analyse vitamin content in roughage at harvest and during storage and to analyze milk vitamin content when feeding the roughage to dairy cows. Roughages produced at five organic dairy farms were monitored at harvest and several times during winter as...... of α-tocopherol was 876 mg per cow–431 mg from roughages, 89 mg from concentrates and 356 mg from a vitamin supplement. Milk yield was 25.9 kg energy-corrected milk (ECM) per cow per day with α-tocopherol and β-carotene contents (μg/ml) of 0.82 and 0.17. The study additionally showed the following...... tendency, but due to few observations no final conclusions could be drawn: •For grass–clover silage there were generally no losses of vitamins during the ensiling process and during storage, but there were huge variations between farms. •For wholecrop silage there was a loss of vitamins during the ensiling...

  19. Storage and treatment of SNF of Alfa class nuclear submarines: current status and problems

    International Nuclear Information System (INIS)

    Available in abstract form only. Full text of publication follows: The current status and main problems associated with storage, defueling and following treatment of spent nuclear fuel (SNF) of Nuclear Submarines (NS) with heavy liquid metal cooled reactors are considered. In the final analysis these solutions could be realized in the form of separate projects to be funded through national and bi- and multilateral funding in the framework of the international collaboration of the Russian Federation on complex utilization of NS and rehabilitation of contaminated objects allocated in the North-West region of Russia. (authors)

  20. Plasma Induced Line Shifts in Emission of Aluminum He alfa Group

    Czech Academy of Sciences Publication Activity Database

    Renner, Oldřich; Krouský, Eduard; Adámek, P.; Dalimier, E.; Angelo, P.; Dufour, E.; Poquerusse, A.; Sauvan, P.; Schott, R.; Rosmej, F.B.; Calisti, A.; Krasniqi, F.; Forster, E.

    Paris : FRONTIER GROUP, 2004 - (Dalimier, E.), s. 175-182 ISBN 2-914601-14-X. [International Conference on Spectral Line Shapes /17./. Paris (FR), 21.06.2004-25.06.2004] R&D Projects: GA MŠk(CZ) LN00A100 Grant ostatní: EC programme IHRP(XE) 1999-0052; Science Project(DE) Fo 186/4-3; Marie Curie Fellowship(XE) HPMF-CT-2002-01550 Institutional research plan: CEZ:AV0Z1010921 Keywords : laser-produced plasma * x-ray spectroscopy * genetic algorithm code * plasma emission theory Subject RIV: BH - Optics, Masers, Lasers

  1. Generalized Quaternion and Rotation in 3-space E (3-alfa,beta)

    CERN Document Server

    Jafari, Mehdi

    2012-01-01

    The paper explains how a unit generalized quaternion is used to represent a rotation of a vector in 3-dimensional space. We review of some algebraic properties of generalized quaternions and operations between them and then show their relation with the rotation matrix.

  2. Plasmacytoid dendritic cell dynamics tune interferon-alfa production in SIV-infected cynomolgus macaques.

    Directory of Open Access Journals (Sweden)

    Timothée Bruel

    2014-01-01

    Full Text Available IFN-I production is a characteristic of HIV/SIV primary infections. However, acute IFN-I plasma concentrations rapidly decline thereafter. Plasmacytoid dendritic cells (pDC are key players in this production but primary infection is associated with decreased responsiveness of pDC to TLR 7 and 9 triggering. IFNα production during primary SIV infection contrasts with increased pDC death, renewal and dysfunction. We investigated the contribution of pDC dynamics to both acute IFNα production and the rapid return of IFNα concentrations to pre-infection levels during acute-to-chronic transition. Nine cynomolgus macaques were infected with SIVmac251 and IFNα-producing cells were quantified and characterized. The plasma IFN-I peak was temporally associated with the presence of IFNα(+ pDC in tissues but IFN-I production was not detectable during the acute-to-chronic transition despite persistent immune activation. No IFNα(+ cells other than pDC were detected by intracellular staining. Blood-pDC and peripheral lymph node-pDC both lost IFNα(- production ability in parallel. In blood, this phenomenon correlated with an increase in the counts of Ki67(+-pDC precursors with no IFNα production ability. In tissues, it was associated with increase of both activated pDC and KI67(+-pDC precursors, none of these being IFNα(+ in vivo. Our findings also indicate that activation/death-driven pDC renewal rapidly blunts acute IFNα production in vivo: pDC sub-populations with no IFNα-production ability rapidly increase and shrinkage of IFNα production thus involves both early pDC exhaustion, and increase of pDC precursors.

  3. Primary cutaneous follicle center lymphoma responsive to interferon alfa-2a

    Directory of Open Access Journals (Sweden)

    Mukaddes Kavala

    2011-01-01

    Full Text Available Follicular lymphoma is the most common type of primary cutaneous B-cell lymphomas with a predilection for the scalp, forehead, and trunk. Herein we report a case of primary cutaneous follicle center lymphoma on the scalp of 72-year-old female. The diagnosis was made histopathologically, confirming the presence of centrocytes and centroblasts. Complete resolution was achieved following administration of subcutaneous interferon α-2a at a dose of 4.5 × 10 6 IU three times weekly for 3 months.

  4. Analisa Kadar Alfa Selulosa Dari Tandan Kosong Kelapa Sawit Dengan Metode Gravimetri

    OpenAIRE

    Utami, Dina Rizkha

    2011-01-01

    Has done an analysis of alpha cellulose content of oil palm empty fruit bunches by gravimetric method. Levels of alpha cellulose was analyzed by soaking cellulose in cold 17,5% NaOH, then in the pressed with a spatula. The sample was filtered with a cup of masir, the residue was washed with 8,3% NaOH and distilled water. Residues in soak for a while with acetic acid and then washed with hot water and alcohol. The residue was dried in the oven and let cool. Residues then weighed and the weight...

  5. A Randomized, Double-Blind, Placebo-Controlled Assessment of BMS-936558, a Fully Human Monoclonal Antibody to Programmed Death-1 (PD-1), in Patients with Chronic Hepatitis C Virus Infection

    Science.gov (United States)

    Gardiner, David; Lalezari, Jay; Lawitz, Eric; DiMicco, Michael; Ghalib, Rheem; Reddy, K. Rajender; Chang, Kyong-Mi; Sulkowski, Mark; Marro, Steven O’; Anderson, Jeffrey; He, Bing; Kansra, Vikram; McPhee, Fiona; Wind-Rotolo, Megan; Grasela, Dennis; Selby, Mark; Korman, Alan J.; Lowy, Israel

    2013-01-01

    Expression of the programmed death 1 (PD-1) receptor and its ligands are implicated in the T cell exhaustion phenotype which contributes to the persistence of several chronic viral infections, including human hepatitis C virus (HCV). The antiviral potential of BMS-936558 (MDX-1106) – a fully human anti-PD-1 monoclonal immunoglobulin-G4 that blocks ligand binding – was explored in a proof-of-concept, placebo-controlled single-ascending-dose study in patients (N = 54) with chronic HCV infection. Interferon-alfa treatment-experienced patients (n = 42) were randomized 5∶1 to receive a single infusion of BMS-936558 (0.03, 0.1, 0.3, 1.0, 3.0 mg/kg [n = 5 each] or 10 mg/kg [n = 10]) or of placebo (n = 7). An additional 12 HCV treatment-naïve patients were randomized to receive 10 mg/kg BMS-936558 (n = 10) or placebo (n = 2). Patients were followed for 85 days post-dose. Five patients who received BMS-936558 (0.1 [n = 1] or 10 mg/kg) and one placebo patient achieved the primary study endpoint of a reduction in HCV RNA ≥0.5 log10 IU/mL on at least 2 consecutive visits; 3 (10 mg/kg) achieved a >4 log10 reduction. Two patients (10 mg/kg) achieved HCV RNA below the lower limit of quantitation (25 IU/mL), one of whom (a prior null-responder) remained RNA-undetectable 1 year post-study. Transient reductions in CD4+, CD8+ and CD19+ cells, including both naïve and memory CD4+ and CD8+ subsets, were observed at Day 2 without evidence of immune deficit. No clinically relevant changes in immunoglobulin subsets or treatment-related trends in circulating cytokines were noted. BMS-936558 exhibited dose-related exposure increases, with a half-life of 20–24 days. BMS-936558 was mostly well tolerated. One patient (10 mg/kg) experienced an asymptomatic grade 4 ALT elevation coincident with the onset of a 4-log viral load reduction. Six patients exhibited immune-related adverse events of mild-to-moderate intensity, including two cases of

  6. A randomized, double-blind, placebo-controlled assessment of BMS-936558, a fully human monoclonal antibody to programmed death-1 (PD-1, in patients with chronic hepatitis C virus infection.

    Directory of Open Access Journals (Sweden)

    David Gardiner

    Full Text Available Expression of the programmed death 1 (PD-1 receptor and its ligands are implicated in the T cell exhaustion phenotype which contributes to the persistence of several chronic viral infections, including human hepatitis C virus (HCV. The antiviral potential of BMS-936558 (MDX-1106 - a fully human anti-PD-1 monoclonal immunoglobulin-G4 that blocks ligand binding - was explored in a proof-of-concept, placebo-controlled single-ascending-dose study in patients (N = 54 with chronic HCV infection. Interferon-alfa treatment-experienced patients (n = 42 were randomized 5∶1 to receive a single infusion of BMS-936558 (0.03, 0.1, 0.3, 1.0, 3.0 mg/kg [n = 5 each] or 10 mg/kg [n = 10] or of placebo (n = 7. An additional 12 HCV treatment-naïve patients were randomized to receive 10 mg/kg BMS-936558 (n = 10 or placebo (n = 2. Patients were followed for 85 days post-dose. Five patients who received BMS-936558 (0.1 [n = 1] or 10 mg/kg and one placebo patient achieved the primary study endpoint of a reduction in HCV RNA ≥0.5 log10 IU/mL on at least 2 consecutive visits; 3 (10 mg/kg achieved a >4 log10 reduction. Two patients (10 mg/kg achieved HCV RNA below the lower limit of quantitation (25 IU/mL, one of whom (a prior null-responder remained RNA-undetectable 1 year post-study. Transient reductions in CD4(+, CD8(+ and CD19(+ cells, including both naïve and memory CD4(+ and CD8(+ subsets, were observed at Day 2 without evidence of immune deficit. No clinically relevant changes in immunoglobulin subsets or treatment-related trends in circulating cytokines were noted. BMS-936558 exhibited dose-related exposure increases, with a half-life of 20-24 days. BMS-936558 was mostly well tolerated. One patient (10 mg/kg experienced an asymptomatic grade 4 ALT elevation coincident with the onset of a 4-log viral load reduction. Six patients exhibited immune-related adverse events of mild-to-moderate intensity, including two cases of

  7. Blood and Milk Protein Polymorphism and Their Effects on Various Production Traits in Simmental Cows

    OpenAIRE

    ŞEKERDEN, Özel

    1998-01-01

    This research was carried out to determine the transferrin (Tf) and haemoglobine (Hb) and milk protein (alfa-S1-casein, beta casein, capa casein, beta lactoglobuline) types, and relationships between Tf and Hb types and milk protein genotypes with various characteristics. The relationship between Tf types and milk yields of control milking and control day were only statistically significant (P0.05). None of the investigated characteristics were effected by milk protein genetic variants at sta...

  8. Quality of Original and Biosimilar Epoetin Products

    OpenAIRE

    2010-01-01

    ABSTRACT Purpose To compare the quality of therapeutic erythropoietin (EPO) products, including two biosimilars, with respect to content, aggregation, isoform profile and potency. Methods Two original products, Eprex (epoetin alfa) and Dynepo (epoetin delta), and two biosimilar products, Binocrit (epoetin alfa) and Retacrit (epoetin zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis and (5) in-vivo potency...

  9. Synthesis of symmetrical 2,2',4,4'-tetrasubstituted [4,4'-bithiazole]-5,5'(4H,4'H)-diones and their reactions with some nucleophiles

    DEFF Research Database (Denmark)

    Andersen, Kenneth K.; Bray, Diana D.; Kjær, Anders;

    1997-01-01

    ,4'-dibenzyl-[4,4'-bithiazole]-5,5'(4H,4'H)-dione were treated with nocleophiles. The former gave imide derivatives of alfa,alfa-dehydrodimeric amino acids when the nucleophile was L-alanine ethyl ester or 1-butylamine. The structure of one of the reaction products, (4R*,5R*)-2,5-diphenyl-2-thiazoline-4......-carboxylic acid and piperidylamide, was established by X-ray crystallography. Treatment of stereoisomeric mixtures of 2,2'-diethoxy-4,4'-bithiazolones with HCl in benzene gave the corresponding racemic and meso bis-(N-carboxythioanhydride)s. A stereoisomeric mixture of the bis(N-carboxythioanhydride)s of...... leucine treated with glycine ethyl ester gave a bicyclic derivative of the alfa,alfa-dehydrodimeric amino acid....

  10. Juba teine "1+1 = 1"

    Index Scriptorium Estoniae

    2004-01-01

    15. III Okupatsioonimuuseumis toimuva "1+1=1" teise ürituse kavast. Presentatsioonide õhtu keskendub konkreetse ruumikogemuse vahendamisele kunstis (helis, slaidi-installatsioonis, tänava-aktsioonis, skulptuuris, videos, filmis). Esinevad Cevdet Erek, Andres Lõo, Andres Kurg (arhitektuur ja interjöörid Marko Raadi filmis "Agent Sinikael") jt.

  11. Resposta virológica sustentada em pacientes com co-infecção pelos genótipos 1 e 2 do vírus da hepatite C, com apenas nove semanas de terapêutica antiviral: relato de caso Sustained virological response in patients with coinfection by hepatitis C virus genotypes 1 and 2, after just nine weeks of antiviral therapy: case report

    Directory of Open Access Journals (Sweden)

    Ana Ruth Araújo

    2010-10-01

    Full Text Available Relata-se um paciente do sexo masculino com 67 anos e sorologia positiva para o vírus da hepatite C (HCV. Exames moleculares revelaram a presença do RNA do HCV, com carga viral de 2.000 cópias/mL e genótipos 1 e 2. O tratamento foi com alfapeginterferon-2a, 180mcg/semana e ribavirina, 1.000mg/dia. Na quarta semana de tratamento, a carga viral para o HCV era indetectável. Na nona semana, o paciente apresentou hematêmese, piora do quadro de astenia, inapetência e comprometimento do estado geral, quando o tratamento foi descontinuado. O PCR foi negativo após 6 meses e permaneceu assim após um ano. O paciente encontra-se assintomático.A report of a 67 year-old male patient with positive serology for HCV. PCR revealed the presence of HCV RNA, viral load of 2,000 copies/mL and genotypes 1 and 2. The pacient was treated with peginterferon alfa-2a at 180mcg/week and ribavirin at 1,000mg/day. In week four of treatment, HCV viral load was undetectable. In week nine, the patient developed hematemesis, worsening of asthenia, anorexia and impaired general condition, so the treatment was discontinued. The PCR was negative six months and one year after the cessation of treatment. The patient remains asymptomatic.

  12. Cirurgia redutora de volume pulmonar: critérios de seleção de pacientes no Hospital Universitário Antônio Pedro, Universidade Federal Fluminense, Niterói, Rio de Janeiro, RJ Lung volume reduction surgery (LVRS: criteria for selecting patient in Antonio Pedro Hospital, Federal Fluminense University, Niterói city, Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Rodolfo Fred Behrsin

    2003-09-01

    Full Text Available O tratamento clínico da doença pulmonar obstrutiva crônica (DPOC está bem padronizado por consensos nacionais e internacionais. Junto com transplante pulmonar, a cirurgia redutora de volume pulmonar (CRVP é alternativa de tratamento cirúrgico para o enfisema. OBJETIVOS: Avaliar critérios de inclusão e exclusão para realização de CRVP em pacientes com DPOC. MÉTODOS: Trinta e um pacientes encaminhados e acompanhados no Ambulatório Multidisciplicar de Pesquisa em DPOC e Tabagismo dos Cursos de Pós-Graduação em Pneumologia da Universidade Federal Fluminense, submetidos a um protocolo de avaliação para seleção de candidatos à CRVP que incluiu anamnese e exame físico padronizados, espirometria com curva fluxo volume e teste com broncodilatador, gasometria arterial com paciente respirando ar ambiente, teste de caminhada por seis minutos (TC6M, radiografia convencional de tórax em póstero anterior e perfil esquerdo, tomografia computadorizada de tórax de alta resolução (TCAR e cintilografia pulmonar de ventilação e perfusão. Análise estatística: descritiva. RESULTADOS: Contra-indicações para CRVP: bronquite crônica (29% ou nove pacientes, VEF 1> 80,0% do previsto (13% ou quatro pacientes, recusa em realizar a cirurgia (10% ou três pacientes, áreas de fibrose, depressão e bronquiectasias (dois pacientes cada, nódulo pulmonar solitário neoplásico, obesidade mórbida, enfisema bolhoso grave, hipertensão pulmonar, passado de ressecção pulmonar, tabagismo corrente, enfisema difuso com deficiencia de alfa-1-antitripsina e doença cardiaca grave (um paciente cada. Um dos pacientes selecionados concordou e foi submetido à CRVP. Sua indicação foi enfisema pulmonar heterogênio com predomínio nos lobos superiores, diagnosticado pela TCAR e cintilografia pulmonar. CONCLUSÕES: Bronquite crônica representou a principal contra-indicação em 29% dos casos. Pacientes com indicação de CRVP, freqüentemente, apresentam

  13. Proteômica na sepse: estudo piloto Proteomics in sepsis: a pilot study

    Directory of Open Access Journals (Sweden)

    Rita Azevedo de Paiva

    2010-12-01

    Full Text Available Na sepse ocorre desregulação da expressão gênica. Os marcadores genéticos revelam apenas o genótipo do indivíduo, não sendo afetados pelos processos biológicos decorrentes da ação do ambiente, estes expressos nas proteínas. Este estudo teve como objetivo alcançar maior compreensão sobre as bases moleculares da sepse. Para tal realizou a identificação e análise da expressão diferencial de proteínas no soro de paciente séptico em diferentes estágios de gravidade (sepse, sepse grave e choque séptico através de técnicas proteômicas. Amostras de soro referentes a cada estágio da sepse foram colhidas e submetidas à eletroforese unidimensional em fitas com gradiente de pH imobilizado seguida de eletroforese bidimensional em gel de poliacrilamida 12,5%. Os géis obtidos foram corados, escaneados e analisados através do programa ImageMasterPlatinum. As proteínas expressas diferencialmente nos géis foram excisadas, digeridas com tripsina e identificadas através de espectrometria de massa. Foram identificadas 14 proteínas expressas diferencialmente entre os estágios da sepse, assim como uma proteína não expressa em todos os estágios, sugerindo a existência de um possível biomarcador. Foram elas: amilóide sérico A, apolipoproteína A-1 (2 isoformas, proteína dedo de zinco 222, albumina humana, PRO 2619, imunoglobulina de cadeia leve kappa região VLJ, imunoglobulina M monoclonal de aglutinação a frio e 7 inibidoras de proteases - alfa-1 antitripsina. Os resultados obtidos neste estudo piloto demonstram a participação das vias do complemento e coagulação, do metabolismo lipídico e da informação genética na sepse. A grande maioria de proteínas identificadas está envolvida no sistema imune com predomínio das proteínas inibidoras de proteases.Gene expression is disrupted by sepsis. Genetic markers can only reveal a patient's genotype, and they are not affected by environmental biological processes. These

  14. Leucograma e teores plasmáticos de proteínas de fase aguda de eqüinos portadores de abdômen agudo e submetidos à laparotomia Leukogram and plasma acute phase protein concentrations in horses with acute abdomen submitted to treatment by laparotomy

    Directory of Open Access Journals (Sweden)

    J.J. Fagliari

    2008-04-01

    Full Text Available Foram examinados 20 eqüinos adultos portadores de abdômen agudo e submetidos à laparotomia. Dez recuperaram-se sem intercorrência pós-operatória (G1 e 10 foram a óbito sete a 10 dias após a cirurgia, com sinais de choque séptico (G2. Avaliaram-se temperatura retal, freqüências cardíaca e respiratória, tempo de preenchimento capilar e teores plasmáticos das proteínas de fase aguda - fibrinogênio, ceruloplasmina, proteína C-reativa, antitripsina, haptoglobina e glicoproteína ácida -, antes e até sete dias após a laparotomia. As leucometrias às 72h e no sétimo dia pós-operatório dos eqüinos que foram a óbito foram, respectivamente, 34,6% e 57,1%, mais altas que a dos animais curados. Os maiores valores de proteína de fase aguda ocorreram no sétimo dia após a cirurgia; os percentuais de elevação de fibrinogênio, antitripsina, glicoproteina ácida, proteína C-reativa, ceruloplasmina e haptoglobina de eqüinos do G2 em relação ao G1 foram 46,8%, 67,9%, 91,9%, 112,2%, 126,9% e 186,2%, respectivamente.Twenty adult horses with acute abdomen were examined and submitted to treatment by laparotomy; ten had no postoperative complication (group 1, and ten showed septic shock symptom and died from seven to ten days after surgery (group 2. Body temperature, heart and respiratory rates, filling capillary time, and plasma acute phase protein concentrations - fibrinogen, ceruloplasmin, C-reactive protein, antitrypsin, haptoglobin, and acid glycoprotein - were evaluated before laparotomy and until seven days after surgery. White blood cell counts at 72h and seven days after surgery in group 2 animals were, respectively, 34.6% and 57.1%, and were higher than those measured in group 1 horses. The highest values of acute phase proteins occurred on the seventh day after surgery. The increase percentages of fibrinogen, antitrypsin, acid glycoprotein, C-reactive protein, ceruloplasmin, and haptoglobin plasma concentrations in group 2

  15. Drug: D05401 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D05401 Drug Octocog alfa (genetical recombination) (JAN); Octocog alfa (INN); Kogen...eparations D05401 Octocog alfa (genetical recombination) (JAN); Octocog alfa (INN) CAS: 139076-62-3 PubChem: 17398295 ...

  16. Shootin1

    DEFF Research Database (Denmark)

    Toriyama, Michinori; Shimada, Tadayuki; Kim, Ki Bum;

    2006-01-01

    novel protein, named shootin1, that became up-regulated during polarization of hippocampal neurons and began fluctuating accumulation among multiple neurites. Eventually, shootin1 accumulated asymmetrically in a single neurite, which led to axon induction for polarization. Disturbing the asymmetric...... organization of shootin1 by excess shootin1 disrupted polarization, whereas repressing shootin1 expression inhibited polarization. Overexpression and RNA interference data suggest that shootin1 is required for spatially localized phosphoinositide-3-kinase activity. Shootin1 was transported anterogradely to the...... growth cones and diffused back to the soma; inhibiting this transport prevented its asymmetric accumulation in neurons. We propose that shootin1 is involved in the generation of internal asymmetric signals required for neuronal polarization....

  17. ASC-1

    DEFF Research Database (Denmark)

    Jakimoski, Goce; Khajuria, Samant

    2011-01-01

    . Unfortunately, the use of a block cipher as a building block limits the performance of the authenticated encryption schemes to at most one message block per block cipher evaluation. In this paper, we propose the authenticated encryption scheme ASC-1 (Authenticating Stream Cipher One). Similarly to LEX, ASC-1...... uses leak extraction from diÆerent AES rounds to compute the key material that is XOR-ed with the message to compute the ciphertext. Unlike LEX, the ASC-1 operates in a CFB fashion to compute an authentication tag over the encrypted message. We argue that ASC-1 is secure by reducingits (IND-CCA , INT...

  18. Heteroepitaxy of CdTe(1 1 1)B on Si(1 1 1) : As

    Science.gov (United States)

    Schick, H.; Bensing, F.; Hilpert, U.; Richter, U.; Hansen, L.; Wagner, J.; Wagner, V.; Geurts, J.; Waag, A.; Landwehr, G.

    2000-06-01

    In order to improve the structural quality of CdTe/Si composite substrates, we have investigated the MBE growth mechanisms of CdTe(1 1 1) onto planar and vicinal arsenic-passivated Si(1 1 1) surfaces. The films were characterized by in situ RHEED, X-ray diffraction, Raman spectroscopy, photoluminescence, secondary electron microscopy, transmission electron microscopy and atomic force microscopy. Rocking curves had peaks narrower than 100 arcsec at a layer thickness of only 1-2 μm. BeTe buffer layers did not show a dominant effect, whereas the twin content decreased drastically when misoriented substrates were used. Efficient twin suppression can be obtained by realizing an interface step alignment between substrate and epitaxial CdTe film.

  19. Neurofibromatosis-1

    Science.gov (United States)

    ... of skin ( subcutaneous tissue) Nerves from the brain (cranial nerves) and spinal cord (spinal root nerves) Causes NF1 ... affect the appearance and put pressure on nearby nerves or organs Many soft tumors on the skin or deeper in ...

  20. 1/N

    International Nuclear Information System (INIS)

    There exist families of field theories with symmetry group SO(N) (or SU(N)) that become simpler as N becomes larger. More precisely, the solutions to these theories possess an expansion in powers of 1/N. This expansion is the subject of these lectures. The 1/N expansion can be used to analyze model field theories. The 1/N expansion is developed for phi4 theory and applied to two two-dimensional models with similar combinatoric structures, the Gross-Neveu model and the CP/sup N-1/ model. These models display (in the leading 1/N approximation) such interesting phenomena as asymptotic freedom, dynamical symmetry breaking, dimensional transmutation, and non-perturbative confinement. It is possible that the 1/N expansion, with N the number of colors, might fruitfully be applied to quantum chromodynamics. Unfortunately, it is not possible to make a decisive test of the approximation, because no one knows how to compute even the first term in the expansion in closed form. However, it is possible to argue that this first term, whatever its detailed form, has many properties that are also shared by the real world, and which are otherwise underived from field theory. These include the saturation of scattering amplitudes by an infinite number of narrow resonances, the essential feature of dual-resonance models

  1. POLX-1

    International Nuclear Information System (INIS)

    The capabilities of the POLX-1 code which was developed for the purpose of generating polynomial fits to neutron few-group cross sections as functions of soluble boron concentration, fuel temperature, moderator density and exposure in the reactor core are described. The effects of extracting removable burnable poison rods and the insertion of control rods are also parameterized as functions of the core conditions. Input data requirements and the format of the output file generated by POLX-1 are also described. 14 refs

  2. 1. Introduction

    OpenAIRE

    Olaniyi, Rasheed

    2013-01-01

    1.1: Background to the Study According to the Safer Cities Programme of UN-Habitat, crime does not occur spontaneously but it grows out of an unequal and exclusive society, and out of a lack of institutional and social control. African cities could be described as places of fear, where violence and crime rule. As Alan Mabin observed, social life and urban economies are often organised around the fear of violence and crime (Mabin, 2004). The criminal justice system, including the police, court...

  3. Danida1

    DEFF Research Database (Denmark)

    Henriksen, Mogens

    1996-01-01

    At the request of Danish Ministry of Foreign Affairs, Department TSA.4, electrical testing of possibly damaged cables has been evaluated. This matter concerns three different types of cable, which are10 km 30(36) kV XLPE armoured submarine power cable, 1-phase15 km 30(36) kV XLPE armoured...... underground power cable, 1-phase20 km 10(12) kV XLPE armoured underground power cable, 3-phase.The possible damage of said cables took place during shipment from Denmark to Bangladesh....

  4. Pentacene on Au(111), Ag(111) and Cu(111): From physisorption to chemisorption

    Science.gov (United States)

    Lu, Meng-Chao; Wang, Rong-Bin; Yang, Ao; Duhm, Steffen

    2016-03-01

    We measured the electronic and the molecular surface structure of pentacene deposited on the (111)-surfaces of coinage metals by means of ultraviolet photoelectron spectroscopy (UPS) and low-energy electron diffraction (LEED). Pentacene is almost flat-lying in monolayers on all three substrates and highly ordered on Au(111) and on Cu(111). On Ag(111), however, weak chemisorption leads to almost disordered monolayers, both, at room temperature and at 78 K. On Cu(111) pentacene is strongly chemisorbed and the lowest unoccupied molecular orbital becomes observable in UPS by a charge transfer from the substrate. On Ag(111) and Cu(111) multilayers adopt a tilted orientation and a high degree of crystallinity. On Au(111), most likely, also in multilayers the molecular short and long axes are parallel to the substrate, leading to a distinctively different electronic structure than on Ag(111) and Cu(111). Overall, it could be demonstrated that the substrate not only determines the geometric and electronic characteristics of molecular monolayer films but also plays a crucial role for multilayer film growth.

  5. Pentacene on Au(111), Ag(111) and Cu(111): From physisorption to chemisorption

    International Nuclear Information System (INIS)

    We measured the electronic and the molecular surface structure of pentacene deposited on the (111)-surfaces of coinage metals by means of ultraviolet photoelectron spectroscopy (UPS) and low-energy electron diffraction (LEED). Pentacene is almost flat-lying in monolayers on all three substrates and highly ordered on Au(111) and on Cu(111). On Ag(111), however, weak chemisorption leads to almost disordered monolayers, both, at room temperature and at 78 K. On Cu(111) pentacene is strongly chemisorbed and the lowest unoccupied molecular orbital becomes observable in UPS by a charge transfer from the substrate. On Ag(111) and Cu(111) multilayers adopt a tilted orientation and a high degree of crystallinity. On Au(111), most likely, also in multilayers the molecular short and long axes are parallel to the substrate, leading to a distinctively different electronic structure than on Ag(111) and Cu(111). Overall, it could be demonstrated that the substrate not only determines the geometric and electronic characteristics of molecular monolayer films but also plays a crucial role for multilayer film growth. (paper)

  6. Pentacene on Au(111), Ag(111) and Cu(111): From physisorption to chemisorption.

    Science.gov (United States)

    Lu, Meng-Chao; Wang, Rong-Bin; Yang, Ao; Duhm, Steffen

    2016-03-01

    We measured the electronic and the molecular surface structure of pentacene deposited on the (111)-surfaces of coinage metals by means of ultraviolet photoelectron spectroscopy (UPS) and low-energy electron diffraction (LEED). Pentacene is almost flat-lying in monolayers on all three substrates and highly ordered on Au(111) and on Cu(111). On Ag(111), however, weak chemisorption leads to almost disordered monolayers, both, at room temperature and at 78 K. On Cu(111) pentacene is strongly chemisorbed and the lowest unoccupied molecular orbital becomes observable in UPS by a charge transfer from the substrate. On Ag(111) and Cu(111) multilayers adopt a tilted orientation and a high degree of crystallinity. On Au(111), most likely, also in multilayers the molecular short and long axes are parallel to the substrate, leading to a distinctively different electronic structure than on Ag(111) and Cu(111). Overall, it could be demonstrated that the substrate not only determines the geometric and electronic characteristics of molecular monolayer films but also plays a crucial role for multilayer film growth. PMID:26870881

  7. Hereditary angioedema type I: a case report

    Directory of Open Access Journals (Sweden)

    Francisca Muñoz Peralta

    2016-03-01

    Full Text Available El angioedema hereditario es una enfermedad rara, de gran heterogeneidad en los síntomas, manifestándose con edema a nivel cutáneo, mucosa gastrointestinal y de laringe/faringe. Aunque existen tres variedades, el tipo I es el más frecuente y es provocado por una deficiencia en la síntesis del complemento C1 inhibidor. La gravedad de la clínica, junto a la baja prevalencia de la enfermedad y la necesidad de un tratamiento específico, hacen que el diagnóstico y tratamiento de dicha patología sea aún una asignatura pendiente para el médico de familia en atención primaria. Presentamos el caso de un adolescente varón con déficit de α-1 antitripsina desde los seis meses de edad, con aparición de angioedemas en piernas y brazos a los 11 años, diagnosticado de angioedema hereditario tipo I un año después. El diagnóstico definitivo de la enfermedad permitió instaurar un tratamiento adecuado a su patología, que consiste en la prevención de brotes que puedan comprometer la vida del paciente y, en el caso de que aparezcan, en la administración del complemento C1 inhibidor.

  8. Dynamics Explorer-1 (DE-1)

    Science.gov (United States)

    Gordon, F.; Pashby, P.

    1991-01-01

    The Dynamics Explorer (DE) mission is designed to study the Earth's electromagnetic fields at varying heights up to 4 Earth radii. The DE-1 was launched on a Delta 3913 launch vehicle from the Western Test Range and was placed in a 561 x 23279 km orbit with a 90 degree inclination. The Deep Space Network (DSN) began to support this extended mission on February 1, 1985. Coverage consists of five passes per day that last for 45 minutes each. Information is given in tabular form for DSN support, frequency assignments, telemetry, command, and tracking mission responsibility.

  9. Mercury(1)

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    1.Mercury is the planet closest to the sun.It is thefastest moving planet.And it is the second smallestplanet in our solar system. 2.Mercury is very difficult to see.Its orbit isbetween us and the sun.So,we have to look toward thebright light of the sun to see it.The sunlight usuallyhides Mercury.When we can see it,it appears as a fuzzyball.

  10. Figures1

    OpenAIRE

    2014-01-01

    NB. Soulignons que cette carte, établie à partir de statistiques officielles, est en contradiction avec de nombreuses enquêtes locales.Source : Diop Salif, Rekacewicz Philippe, 2003, Atlas mondial de l’eau, Paris, Éditions Autrement.Figure 1. Part de la population urbaine ayant accès à l'eau potable en Afrique subsaharienne Source : Diop, Rekacewicz, 2003.Figure 2. Afrique subsaharienne : pénurie d'eau douce et stress hydrique en 1990 et 2025 (projection) Source : Diop, Rekacewicz, 2003.Fig...

  11. Wetlands; 1 : 1 000 000

    International Nuclear Information System (INIS)

    The most valuable swamps and other hydrophilic associations represent wetlands. In the years 1991 - 2000, 261 specialists carried out their inventory. Apart from these localities another 1,050 locally important wetlands were identified while more than 500 wetlands were identified geographically, i.e. without characterisation of flora and fauna, which prevented their categorisation. The source material used for mapping of wetlands was provided by the Centre for Wetland Mapping of the Slovak Union of Nature and Landscape Conservationists in Prievidza. Mapping of wetlands in Slovakia continues and its main aim is to: (1) cartographically process the yet not investigated territories, for instance, the districts of Humenne, Zlate Moravce, and other; (2) explore fauna and flora of more than 500 geographically identified wetlands; (3) update the state of biota of internationally and nationally important wetlands; (4) involve more specialists in inventory of wetlands. (authors)

  12. 26 CFR 1.7872-1-1.7872-4 - [Reserved

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 13 2010-04-01 2010-04-01 false 1.7872-1-1.7872-4 Section 1.7872-1-1.7872-4 Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES General Actuarial Valuations §§ 1.7872-11.7872-4...

  13. Drug: D06120 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D06120 Drug Thyrotropin alfa (genetical recombination) (JAN); Thyrotropin alfa (USA...N/INN); Thyrotropin human alfa (genetical recombination); Thyrogen (TN) Thyroid hormone Therapeutic category... Other agents not mainly for therapeutic purpose 799 Miscellaneous 7990 Miscellaneous D06120 Thyrotropin alfa (genetica...UES H01AB Thyrotropin H01AB01 Thyrotropin alfa D06120 Thyrotropin alfa (genetical...NTS V04CJ Tests for thyreoidea function V04CJ01 Thyrotropin D06120 Thyrotropin alfa (genetical recombination

  14. Drug: D03896 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available D03896 Drug Dornase alfa (genetical recombination) (JAN); Dornase alfa (USAN/INN); ...llaneous 2290 Miscellaneous D03896 Dornase alfa (genetical recombination) (JAN); Dornase alfa (USAN/INN) Ana...ytics R05CB13 Dornase alfa (desoxyribonuclease) D03896 Dornase alfa (genetical recombination) (JAN); Dornase alfa (USAN/INN) CAS: 143831-71-4 PubChem: 17397980 DrugBank: DB00003 ...

  15. 1-Benzylimidazolium hexafluorophosphate–1-benzylimidazole (1/1

    Directory of Open Access Journals (Sweden)

    Qing-Xiang Liu

    2008-02-01

    Full Text Available In the title compound, C10H11N2+·PF6−·C10H10N2, the H atom involved in protonation is disordered equally between the cation and the neutral molecule. The dihedral angle between the phenyl and imidazole rings is 82.6 (2°. In the crystal structure, there are head-to-tail π–π stacking interactions between imidazole rings; the interplanar separation is 3.295 (1 Å and the centroid–centroid separation is 3.448 (3 Å. In the centrosymmetric anion, two F atoms are disordered over two positions; the refined site-occupancy factors are 0.855 (11 and 0.145 (11.

  16. Methane dissociation on Pt(1 1 1), Ir(1 1 1) and PtIr(1 1 1) surface: A density functional theory study

    Energy Technology Data Exchange (ETDEWEB)

    Qi, Qiuhong; Wang, Xiujun, E-mail: xjwangcn@scut.edu.cn; Chen, Li; Li, Baitao

    2013-11-01

    A periodic density functional theory (DFT) was utilized to calculate the dissociation process of methane (CH{sub 4}) on Pt(1 1 1), Ir(1 1 1) and PtIr(1 1 1) surfaces. As compared to the adsorption energy, the most stable configurations of methane, methane dissociation species and co-adsorption of CH{sub x} (x = 0–3) with H were obtained. The kinetic results of the CH{sub 4} dissociation indicated that the dissociating of CH{sub 4} into CH{sub 3} and H is the rate-limiting step on the PtIr(1 1 1) and Ir(1 1 1) surfaces. CH was the most abundant species that was difficult to dehydrogenate into C and H. Particularly, the activation barrier for CH{sub 3} → CH{sub 2} + H and CH{sub 2} → CH + H on the Pt(1 1 1) surface is 3.5 and 1.4 times, respectively, higher than that on the PtIr(1 1 1) surface. According to the thermodynamics principles, the successive dehydrogenation of CH{sub 4} preferred to take place on the PtIr surface.

  17. Gateways to clinical trials.

    Science.gov (United States)

    Tomillero, A; Moral, M A

    2010-05-01

    O(6)-Benzylguanine; (-)-Gossypol; Abatacept, AC-2592, Adalimumab, AIDSVAX gp120 B/E, Alemtuzumab, Aliskiren fumarate, ALVAC E120TMG, Ambrisentan, Amlodipine, Anakinra, Aripiprazole, Armodafinil, Atomoxetine hydrochloride, Avotermin; Bevacizumab, BIBW-2992, Bortezomib, Bosentan, Botulinum toxin type B; Canakinumab, CAT-354, Ciclesonide, CMV gB vaccine, Corifollitropin alfa, Daptomycin, Darbepoetin alfa, Dasatinib, Denosumab; EndoTAG-1, Eplerenone, Esomeprazole sodium, Eszopiclone, Etoricoxib, Everolimus, Exenatide, Ezetimibe, Ezetimibe/simvastatin; F-50040, Fesoterodine fumavate, Fondaparinux sodium, Fulvestrant; Gabapentin enacarbil, Golimumab; Imatinib mesylate, Inhalable human insulin, Insulin glargine, Ivabradine hydrochloride; Lercanidipine hydrochloride/enalapril maleate, Levosimendan, Liposomal vincristine sulfate, Liraglutide; MDV-3100, Mometasone furoate/formoterol fumavate, Multiepitope CTL peptide vaccine, Mycophenolic acid sodium salt, Nabiximols, Natalizumab, Nesiritide; Obeticholic acid, Olmesartan medoxomil, Omalizumab, Omecamtiv mecarbil; Paclitaxel-eluting stent, Paliperidone, Pegfilgrastim, Peginterferon alfa-2a, Peginterferon alfa-2b, Peginterferon alfa-2b/ ribavirin, Pemetrexed disodium, Polymyxin B nonapeptide, PORxin-302, Prasugrel, Pregabalin, Pridopidine; Ranelic acid distrontium salt, Rasagiline mesilate, rDEN4delta30-4995, Recombinant human relaxin H2, rhFSH, Rilonacept, Rolofylline, Rosiglitazone maleate/metformin hydrochloride, Rosuvastatin calcium, Rotigotine; Salcaprozic acid sodium salt, Sirolimus-eluting stent, Sitagliptin phosphate monohydrate, Sitaxentan sodium, Sorafenib, Sunitinib malate; Tadalafil, Tapentadol hydrochloride, Temsirolimus, Tenofovir, Tenofovir disoproxil fumarate, Teriparatide, Tiotropium bromide, Tocilizumab, Tolvaptan, Tozasertib, Treprostinil sodium; Ustekinumab; Vardenafil hydrochloride hydrate, Varenicline tartrate, Vatalanib succinate, Voriconazole, Vorinostat; Zotarolimus-eluting stent. PMID:20508873

  18. Tumor necrosis factor-alfa and interleukin-4 in cerbrospinal fluid and plasma in different clinical forms of multiple sclerosis

    Directory of Open Access Journals (Sweden)

    Obradović Dragana

    2012-01-01

    Full Text Available Background/Aim. Multiple sclerosis (MS is an immunemediated central nervous system disease characterized by inflammation, demyelination and axonal degeneration. Cytokines are proven mediators of immunological process in MS. The aim of this study was to investigate whether there is a difference in the production of the tumor necrosis factor alpha (TNF-alpha and interleukin-4 (IL-4 in cerebrospinal fluid (CSF and plasma in the MS patients and the controls (other neurological non-inflammatory diseases and to determine a possible difference in these cytokines in plasma and CSF in different clinical forms of MS. Methods. This study involved 60 consecutive MS patients - 48 patients with relapsing-remitting MS (RRMS and 12 patients with secondary progressive MS (SPMS. The control group consisted of 20, age and sex matched, nonimmunological, neurological patients. According to the clinical presentation of MS at the time of this investigation, 34 (56.7% patients had relapse (RRMS, 14 (23.3% were in remission (RRMS, while the rest of the patients, 12 (20.0%, were SPMS. TNF-alpha and IL-4 concentrations were measured in the same time in CSF and plasma in the MS patients and the controls. Extended disability status score (EDSS, albumin ratio and IgG index were determined in all MS patients. Results. The MS patients had significantly higher CSF and plasma levels of TNF-alpha than the controls (p < 0.001 for both samples. IL-4 CSF levels were significantly lower in the MS patients than in the controls (p < 0.001, however plasma levels were similar. The patients in relapse (RRMS and with progressive disease (SPMS had higher concentrations of CSF TNF-alpha levels than the patients in remission (p < 0.001. IL-4 CSF levels in relapse (RRMS and SPMS groups were lower than in the patients in remission. The patients in remission had an unmeasurable plasma TNF-alpha level and the patients with SPMS had significantly lower IL-4 levels in plasma than the patients in relapse and remission (p < 0.001. The only significant correlation between cytokine level with either EDSS, or albumin ratio, or IgG index, was found between CSF TNF-alpha levels and albumin ratio in the patients with relapse (R square = 0.431, p < 0.001. Conclusion. According to the obtained data MS relapse was characterized by high concentrations of TNF-alpha in CSF and plasma and low concentrations of IL-4 in CSF. Remission was characterized by high concentrations of IL-4 and low concentrations of TNF-alpha both in CSF and plasma. SPMS was characterized with lower concentrations of TNF-alpha and IL-4 compared to relapse, both in CSF and plasma.

  19. Effects of D2-dopamine and alfa-adrenoceptor antagonists in stress induced changes on immune reponsiveness of mice

    Czech Academy of Sciences Publication Activity Database

    Fišerová, Anna; Starec, M.; Kuldová, M.; Kovářů, H.; Páv, M.; Vannucci, Luca; Pospíšil, Miloslav

    2002-01-01

    Roč. 130, - (2002), s. 55-65. ISSN 0165-5728 R&D Projects: GA ČR GA310/98/0347; GA ČR GV312/98/K034; GA ČR GA304/01/0850 Institutional research plan: CEZ:AV0Z5020903 Keywords : restraint stress * cytokine * nk cell Subject RIV: EE - Microbiology, Virology Impact factor: 3.577, year: 2002

  20. Induction; Induccion de la respuesta SOS por radiacion alfa en cepas de Escherichia coli defectuosas en reparacion y recombinacion

    Energy Technology Data Exchange (ETDEWEB)

    Serment G, J.; Brena V, M. [Laboratorio de Genetica Microbiana, Departamento de Biologia, ININ, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    2000-07-01

    At the incidence on biological systems, the ionizing radiation can affect so much its structural components as the genetic material since in a direct form or by the free radicals produced mainly the water radiolysis via (indirect effect). The alpha particles produce a great quantity of leisures in sites very near of them, by consequence results in a major RDB frequency. For establish the influence that would be the leisures concentration (specifically RDB) it was decided to research what occur when is irradiated with high LET corpuscular radiation and major power of ionization using for this alpha particles of an Americium 241 source and Escherichia coli stubs with different defects in reparation genes, recombination and protection to the radiation damage. (Author)