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Sample records for al virus huasteco

  1. Diversidad morfológica del frijol ayocote del Carso Huasteco de México Morphologic diversity of ayocote beans from Huasteco Karst of Mexico

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    Patricia Vargas-Vázquez; José S. Muruaga-Martínez; Sandra E. Martínez-Villarreal; Régulo Ruiz-Salazar; Sanjuana Hernández-Delgado; Netzahualcóyotl Mayek-Pérez

    2011-01-01

    El frijol ayocote (Phaseolus coccineus L.) se cultiva en zonas templadas y subtropicales de México. La colección nacional de la especie incluye 800 accesiones y está resguardada por un banco de germoplasma nacional ubicado en Chapingo, México. En este trabajo se caracterizaron 98 accesiones de frijol ayocote de la subprovincia fisiográfica Carso Huasteco de Puebla, México con base en características fenológicas y del color y dimensión de la semilla. Los colores que predominaron fueron el negr...

  2. Diversidad morfológica del frijol ayocote del Carso Huasteco de México Morphologic diversity of ayocote beans from Huasteco Karst of Mexico

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    Patricia Vargas-Vázquez

    2011-09-01

    Full Text Available El frijol ayocote (Phaseolus coccineus L. se cultiva en zonas templadas y subtropicales de México. La colección nacional de la especie incluye 800 accesiones y está resguardada por un banco de germoplasma nacional ubicado en Chapingo, México. En este trabajo se caracterizaron 98 accesiones de frijol ayocote de la subprovincia fisiográfica Carso Huasteco de Puebla, México con base en características fenológicas y del color y dimensión de la semilla. Los colores que predominaron fueron el negro (N1 y variantes de violeta (5R y de beige (10YR y 7.5YR. Además, se registraron las variables climáticas de los sitios de recolección originales de cada accesión. El germoplasma se separó en 2 grupos: uno incluyó 42 accesiones con ciclo biológico tardío (días a madurez fisiológica > 110, semillas grandes (peso de 100 semillas > 40 g y vainas largas (longitud > 8 cm con 1 a 3 semillas, desarrollados por selección empírica de los agricultores locales en sitios con temperaturas mínimas de 2 a 5 °C, y otro incluyó 56 accesiones con ciclo precoz (≤ 110 días, semilla chica (peso ≤ 40 g y vainas cortas (longitud ≤ 8 cm con 2 a 4 semillas, cultivadas en sitios con temperaturas mínimas de 0 a - 2 °C. El cultivo del frijol ayocote del Carso Huasteco se limita a una superficie relativamente pequeña; no obstante, con base en las formas y épocas de cultivo, se observó notable variación y diferenciación fenotípica en el germoplasma local. Futuros trabajos determinarán las medidas de conservación y aprovechamiento de dicho germoplasma.Ayocote beans (Phaseolus coccineus L. are cultivated in temperate and subtropical regions in Mexico. The national collection of the species includes 800 accessions and it is preserved by one national germplasm bank places in Chapingo, Mexico. In this work 98 ayocote bean accessions from sub-province Huasteco Karst of Puebla, Mexico were characterized based on phenological traits as well as seed color

  3. VIRUS FITOPATÓGENOS EN INSECTOS ASOCIADOS AL AJO

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    Octavio Gustavo García-Rodríguez

    2014-01-01

    Full Text Available La propagación vegetativa del ajo ( Allium sativum es la principal vía de transmisión de virus para este cultivo. No obs - tante, no se debe descartar la dispersión por insectos vectores. El objetivo del presente estudio fue detectar, mediante la prueba de ELISA, la presencia de virus en insectos colectados en plantas de ajo. El experimento se llevó a cabo durante el ciclo Otoño-Invierno 2008-2009. La toma de muestras de insectos se realizó en tres fechas después de la siembra. La identificación de las especies de insectos se realizó utilizando un estereomicroscopio Zeiss de 30X y claves taxonómicas. La detección de virus fue mediante la técnica de DAS-ELISA para los potyvirus Virus del rayado amarillo del puerro ( Leek yellow spot virus : LYSV y Virus del enanismo amarillo de la cebolla ( Onion yellow dwarf virus: OYDV; los carlavirus Virus latente común del ajo ( Garlic common latent virus : GCLV y Virus latente del chalote ( Shallot latent virus : SLV; y el tospovirus Virus del manchado amarillo del iris ( Iris yellow spot virus : IYSV. Se identificaron 19 especies de insectos, de los que destacaron Thrips tabaci Lindeman, como positivo en 18 muestras para GCLV y dos muestras para IYSV, y Collops quadrimaculatus , como positivo en una muestra para GCLV.

  4. Co-circulation of Dengue and Chikungunya Viruses, Al Hudaydah, Yemen, 2012

    OpenAIRE

    Rezza, Giovanni; El-Sawaf, Gamal; Faggioni, Giovanni; Vescio, Fenicia; Al Ameri, Ranya; De Santis, Riccardo; Helaly, Ghada; Pomponi, Alice; Metwally, Dalia; Fantini, Massimo; Qadi, Hussein; Ciccozzi, Massimo; Lista, Florigio

    2014-01-01

    We investigated 400 cases of dengue-like illness in persons hospitalized during an outbreak in Al Hudaydah, Yemen, in 2012. Overall, 116 dengue and 49 chikungunya cases were diagnosed. Dengue virus type 2 was the predominant serotype. The co-circulation of these viruses indicates that mosquitoborne infections represent a public health threat in Yemen.

  5. Exposición ocupacional al virus de hepatitis C Occupational exposure to hepatitis C virus

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    Eduardo Warley

    2006-04-01

    Full Text Available La transmisión ocupacional de virus de hepatitis C (VHC es un área de creciente preocupación dada la falta de profilaxis y la poca información de su prevalencia en el medio hospitalario. Sobre 128 exposiciones ocupacionales ocurridas en el Hospital Diego Paroissien entre 1999 y 2003 hubieron 8 casos de exposición a VHC (6.3% y un caso de seroconversión posterior a la exposición (0.8%. No existiendo en la actualidad terapia preventiva para VHC resulta de gran interés la posibilidad de tratamiento de la infección aguda. La mayor parte de los autores coincide en recomendar el tratamiento del episodio agudo de hepatitis por VHC basado en la evidencia actual, aunque aún no está bien definida la mejor estrategia diagnóstica y terapéutica. El acatamiento de las Normas de Precauciones Universales sigue siendo en la actualidad la más importante medida preventiva para evitar la infección ocupacional por VHC en el personal de salud y la de mejor equilibrio costo-beneficio.Occupational exposure to Hepatitis C virus is an area of growing concern due to a lack of prophylaxis and limited knowledge regarding prevalence in hospital environment. Between 1999 and 2003, a total of 128 occupational exposures to this virus were registered in Diego Paroissien Hospital, eight of which led to hepatitis (6.3% and one case to serum conversion (0.8%. Currently there is no preventive therapy and great interest is focused on acute infection therapy the effectiveness of which is still controversial. This study reinforces the fact that adherence to the Universal Precautions is still the most important preventive measure for health care workers, and the most cost beneficial.

  6. Exposición ocupacional al virus de hepatitis C Occupational exposure to hepatitis C virus

    OpenAIRE

    Eduardo Warley; Javier Desse; Edgardo Szyld; Fernando Silva Nieto; Silvia Cetani; Nancy Pereyra; Adriana De Luca; Alejandra Gurtman

    2006-01-01

    La transmisión ocupacional de virus de hepatitis C (VHC) es un área de creciente preocupación dada la falta de profilaxis y la poca información de su prevalencia en el medio hospitalario. Sobre 128 exposiciones ocupacionales ocurridas en el Hospital Diego Paroissien entre 1999 y 2003 hubieron 8 casos de exposición a VHC (6.3%) y un caso de seroconversión posterior a la exposición (0.8%). No existiendo en la actualidad terapia preventiva para VHC resulta de gran interés la posibilidad de trata...

  7. Respuesta neuroinmunológica en la encefalitis asociada al virus del dengue

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    Bárbara Padilla-Docal

    2013-12-01

    Full Text Available El virus del dengue es un virus ARN miembro de la familia Flaviviridae, la cual incluye, además, el de la fiebre amarilla, el del Nilo del Oeste y la encefalitis japonesa. Se realizó un estudio retrospectivo con tres pacientes diagnosticados de encefalitis asociada al dengue, en cuyas muestras de suero y líquido cefalorraquídeo se cuantificaron los niveles de las clases mayores de inmunoglobulinas por inmunodifusión radial y la manosa de unión a lectina, proteína de la vía de las lectinas del sistema del complemento por fluorometría. En el reibergrama se muestra la presencia de síntesis intratecal de las tres clases de inmunoglobulinas y ausencia de síntesis intratecal de lectina de unión a manosa. Existieron diferencias en cuanto al por ciento de síntesis intratecal de inmunoglobulinas, las cuales estuvieron relacionadas con el momento de la infección por el virus y la aparición de las manifestaciones neurológicas compatibles con una encefalitis. Este es el primer reporte de afectaciones neurológicas en pacientes cubanos con dengue. La respuesta inmune intratecal puede ser utilizada para el mejor conocimiento de la enfermedad y contribuir al desarrollo de posibles candidatos vacunales.

  8. Generalidades sobre las partículas similares al Virus del Papiloma Humano

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    Jorge Félix Beltrán Lissabet

    2014-01-01

    Full Text Available El virus del papiloma humano provoca (VPH una de las enfermedades de transmisión sexual más frecuentes a nivel mundial que afecta aproximadamente a 500 000 mujeres cada año. La proteína L1 se encuentra m arcadamente conservada entre los diferentes tipos del VPH, y tiene la capacidad de autoensamblarse formando partículas similares a virus (PSV que presentan u na elevada inmunogenicidad. El uso de PSV recombinantes como inmunógenos, se ha incrementado en lo s últimos años. Las dos vacunas profilácticas actuales contra el VPH (Gardasil y Cervarix están basadas en PSV, las cuales han resultado muy eficac es. Existen disímiles estrategias encaminadas a la obtención de PSV, entre las que se destacan las modifica ciones genéticas, la selección idónea de un sistema de expresión y la optimización de los métodos de purificación. Pese a los resultados en las investigaciones en el campo del desarrollo de PSV del VPH, aún queda mucho por hacer, debido a la influencia de disímiles variables desconocidas que afectan la organización y la actividad biológica de este complejo macromolecular. La purificación de la proteína L1 constituye una etapa clave durante la obtención PSV del VPH, que depende de la estrategia de purificaci ón utilizada. El desarrollo de PSV quiméricas del VPH se ha convertido en una prometedora estrategia que permite el desarrollo de una repue sta humoral heterogénea contra las infecciones provocadas por los diferentes tipos del VPH. Aunque se ha avanzado en las investigaciones encaminadas al desarrollo de las PSV del VPH, aún existen muchas variables desconocidas que influyen de maner a desconocida en el ensamblaje macromolecular de estas estructuras.

  9. Evaluación de accesiones de Capsicum spp. por su reacción al virus del mosaico deformante del pimentón (PepDMV

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    García Davalos Mario Augusto

    2010-03-01

    Full Text Available Se evaluaron 235 accesiones de Capsicum spp. procedentes del Banco de Germoplasma de la Universidad Nacional de Colombia sede Palmira en condiciones de invernadero por su reacción al Virus deformante del Pimentón (PepDMV. Solamente se identificaron 13 accesiones (5% como resistentes al virus, según la ausencia de síntomas y ausencia del virus en pruebas serológicas (PTA-ELISA y RT-PCR. Los materiales resistentes incluyen variedades de C. annuum, C. frutescens, C. chinense y C. baccatum.

  10. Evaluación de accesiones de Capsicum spp. por su reacción al virus del mosaico deformante del pimentón (PepDMV)

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    Catherine Pardey R; Andrés M Posso T; Mario A García D

    2010-01-01

    Se evaluaron 235 accesiones de Capsicum spp. procedentes del Banco de Germoplasma de la Universidad Nacional de Colombia sede Palmira en condiciones de invernadero por su reacción al Virus deformante del Pimentón (PepDMV). Solamente se identificaron 13 accesiones (5%) como resistentes al virus, según la ausencia de síntomas y ausencia del virus en pruebas serológicas (PTA-ELISA y RT-PCR). Los materiales resistentes incluyen variedades de C. annuum, C. frutescens, C. chinense y C. baccatum.

  11. Evaluación de accesiones de Capsicum spp. por su reacción al virus del mosaico deformante del pimentón (PepDMV

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    Catherine Pardey R

    2010-01-01

    Full Text Available Se evaluaron 235 accesiones de Capsicum spp. procedentes del Banco de Germoplasma de la Universidad Nacional de Colombia sede Palmira en condiciones de invernadero por su reacción al Virus deformante del Pimentón (PepDMV. Solamente se identificaron 13 accesiones (5% como resistentes al virus, según la ausencia de síntomas y ausencia del virus en pruebas serológicas (PTA-ELISA y RT-PCR. Los materiales resistentes incluyen variedades de C. annuum, C. frutescens, C. chinense y C. baccatum.

  12. Neoplasias hemopoyéticas en 10 gatos positivos al virus leucemia felina Haemopoyetic neoplasm in 10 cats positive to feline leukaemia virus

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    L. Muñoz

    2005-01-01

    Full Text Available En Chile se diagnosticó la presencia del antígeno del virus leucemia felina en el suero de gatos naturalmente infectados en 1989 mediante la prueba de ELISA. Este virus produce enfermedades neoplásicas como no neoplásicas. Dentro de las neoplasias hemopoyéticas, las linfoproliferativas son más frecuentes que las mieloproliferativas. Para realizar un diagnóstico certero de la línea celular alterada se deben realizar hemogramas y mielogramas con tinciones de rutina (Giemsa y tinciones específicas. Estas últimas se basan en reacciones citoquímicas que permiten identificar los diferentes blastos. El objetivo de este trabajo es contribuir al conocimiento y diagnóstico de las neoplasias hemopoyéticas en gatos positivos a este virus. Para ello se utilizaron 10 gatos positivos al virus leucemia y además con signología clínica a los cuales se les extrajo una muestra de sangre y de médula ósea para realizar dichas tinciones. Las tinciones citoquímicas utilizadas fueron la peroxidasa, cloroacetato naftol AS-D estearasa y acetato alfanaftil estearasa. Además, se utilizaron como controles cuatro gatos clínicamente sanos y negativos al virus leucemia. Al analizar los hemogramas y mielogramas con tinciones corrientes y tinciones citoquímicas se encontró que 7/10 se clasificaron como linfoma, donde tres de ellos además presentaron alteración de la línea granulocítica neutrofílica; 2/ 10 presentaron mielosis eritrémica y 1/10 leucemia eosinofílica. Resultaron una mayor cantidad de células positivas (teñidas con peroxidasa y cloroacetato naftol AS-D estearasa en sangre en los gatos enfermos que en los controles, lo que indica que existe un mayor número de células inmaduras circulando. Los resultados de las tinciones citoquímicas en médula ósea fueron semejantes entre los gatos sanos y enfermos. Algunos puntos que ayudan al diagnóstico de las neoplasias hemopoyéticas son la severidad de la anemia, la aparición de c

  13. Associated Factors to Human Papilloma Virus. A study performed in Health Area V, in Cienfuegos, Cuba. Factores asociados al virus del papiloma humano. Área V. Cienfuegos.

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    Adys Castro Barberena

    2008-06-01

    Full Text Available Background: Human Papilloma Virus has been considered as the precursor of flat lesions in the cervical epithelium which develops Cervix uterine cancer. Objectives: to analyze risk factors associated to infections due to human papilloma virus. Method: An ambispective, descriptive study was carried out from January 2003 to January 2004, taking 60 patients whose Papanicolaou smears were abnormal. All these patients belonged to health area V, in Cienfuegos, Cuba. A guide for interview with different variables was used. Results: infected predominant ages were from 30 to 39 years old. 55% of the patients are married women or with stable sexual relationships. 6 patients comprised between 40 to 59 years old were observed with association to intra epithelium cervix neoplasias. For a 10 % of them, they have had from 3 to 12 sexual partners. The starting age to have sexual intercourse is from 15 to 19 years old. 75 % of the patients are smokers. Conclusions: results coincide with the medical literature reviewed regarding risk factors and its important relationship with NIC and so the cervicouterine cancer genesis.
    Fundamento: El virus del papiloma humano se ha considerado el precursor de lesiones planas en el epitelio cervical las cuales desarrollan el Cáncer Cervico uterino. Objetivo: Analizar los factores de riesgo asociados a la infección por virus del papiloma humano. Método: Se realiza un estudio investigativo, descriptivo, ambiespectivo, a partir de Enero del 2003 a Enero del 2004 comenzando en Enero 2003 estudiando 60 pacientes con citologías anormales, correspondientes al Área V del Municipio de Cienfuegos. Se empleo una guía de entrevistas con diversas variables. Resultados: Las edades predominantes de esta infección es de 30 – 39 años, el 55% son mujeres casadas o con relaciones

  14. Rekombinante Hepatitis B Virus Kapside : Untersuchungen zur Eignung als ikosahedrale Träger für Strukturuntersuchungen, zur in vitro Assemblierung und Nukleinsäureverpackung

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    Vogel, Maren

    2004-01-01

    Das aus 183 aa bestehende Hepatitis B Virus (HBV) Coreprotein bildet ikosahedral symmetrische Kapside aus 180 bzw. 240 identischen Untereinheiten. Rekombinantes Coreprotein (HBc) kann ohne die Beteiligung anderer Proteine zu Kapsid-ähnlichen Partikeln (CLPs) assemblieren, wobei die Bildung von CLPs auch dann erhalten bleibt, wenn Fremdproteine, wie an Hand des Green Fluorescent Protein (GFP) als Modell gezeigt, geeignet an HBc fusioniert werden. HBc-abgeleitete CLPs können somit als ikosahedr...

  15. Técnicas de reproducción asistida en hombres seropositivos al virus de la inmunodeficiencia humana tipo-1 (VIH-1)

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    Marina Rugero, Fernando

    2001-01-01

    En este trabajo se presenta el estudio clínico de 335 hombres seropositivos al VIH-1 que acuden a un centro de reproducción con deseos de tener hijos. Se estudia también cómo influyen los diferentes par metros cl'nicos e inmunológicos relacionados con el SIDA en la calidad seminal de estos hombres. Se describe la técnica de lavado seminal utilizada para eliminar el virus del semen y aislar la fracción de espermatozoides móviles libres de virus para su utilización en TRA. Tras los lavados se a...

  16. Herencia de la resistencia al virus del mosaico deformante del pimentón PepDMV en Capsicum

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    Mario Augusto García Dávila

    2009-10-01

    Full Text Available En busca de determinar el modo de herencia de la resistencia en tres materiales resistentes, se hicieron cruzamientos hacia tres líneas susceptibles. Se siguió el método del retrocruzamiento propuesto por Warner (1952 fundamentado en el modelo matemático de Fisher Immer y Tedin (1932 descrito con detalle por Mater (1949 y Jenkins (1982, 1984 en el cual se descompone la varianza genética en tres componentes: aditiva, dominancia y epistática. El modelo del retrocruzamiento incluye los dos parentales, el hibrido de la primera generación y la autofecunfación de la F1 para formar la población F2 y las retrocruzas hacia ambos padres. Las poblaciones fueron evaluadas a resistencia al virus del mosaico del pimentón PepDMV en condiciones de invernadero. Los resultados mostraron que el modelo aditivodominancia explicó la resistencia en los híbridos formados entre materiales resistentes y susceptibles. Los genes con acción heredable transmiten a la descendencia el efecto de resistencia. La ganancia de la resistencia viral se da por la presencia de parentales resistentes.

  17. Intraparenchymal spinal cord delivery of adeno-associated virus IGF-1 is protective in the SOD1G93A model of ALS

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    Lepore, Angelo C.; Haenggeli, Christine; Gasmi, Mehdi; Bishop, Kathie M.; Bartus, Raymond T.; Maragakis, Nicholas J.; Rothstein, Jeffrey D.

    2007-01-01

    The potent neuroprotective activities of neurotrophic factors, including insulin-like growth factor 1 (IGF-1), make them promising candidates for treatment of amyotrophic lateral sclerosis (ALS). In an effort to maximize rate of motor neuron transduction, achieve high levels of spinal IGF-1, and thus enhance therapeutic benefit, we injected an adeno-associated virus 2 (AAV2)-based vector encoding human IGF-1 (CERE-130) into lumbar spinal cord parenchyma of SOD1G93A mice. We observed robust an...

  18. Caracterización inmunológica de invididuos con exposición sexual persistente al virus de la inmunodeficiencia humana (VIH) que permanecen seronegativos

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    Restrepo González, Clara

    2012-01-01

    Existen individuos que a pesar de repetidas exposiciones al VIH permanecen seronegativos, sin evidencia clínica ni virológica de infección por este virus. A estos individuos se les conoce como “expuestos al VIH que permanecen seronegativos” (HESN; del inglés “HIV-exposed but seronegative”). Dentro de este grupo de sujetos se han descrito trabajadoras sexuales, recién nacidos de madres infectadas, usuarios de drogas intravenosas, trabajadores sanitarios, y parejas homosexuales o he...

  19. Correction: Taliaferro, L. et al. Evaluation of the Broad-Range PCR-Electrospray Ionization Mass Spectrometry (PCR/ESI-MS System and Virus Microarrays for Virus Detection. Viruses 2014, 6, 1876-1896

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    Lanyn P. Taliaferro

    2014-11-01

    Full Text Available We have noted some inaccuracies in Table 1 and Table 2 of our article “Evaluation of the Broad-Range PCR-Electrospray Ionization Mass Spectrometry (PCR/ESI-MS System and Virus Microarrays for Virus Detection” (Viruses 2014, 6, 1876–1896 [1].[...

  20. Evaluación de accesiones de Capsicum spp. por su reacción al virus del mosaico deformante del pimentón (PepDMV) Screening of Capsicum spp. to the deforming mosaic virus from pepper (PepDMV)

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    Catherine Pardey R; Andrés M Posso T; Mario A García D

    2010-01-01

    Se evaluaron 235 accesiones de Capsicum spp. procedentes del Banco de Germoplasma de la Universidad Nacional de Colombia sede Palmira en condiciones de invernadero por su reacción al Virus deformante del Pimentón (PepDMV). Solamente se identificaron 13 accesiones (5%) como resistentes al virus, según la ausencia de síntomas y ausencia del virus en pruebas serológicas (PTA-ELISA y RT-PCR). Los materiales resistentes incluyen variedades de C. annuum, C. frutescens, C. chinense y C. baccatum.A t...

  1. Presencia de Diferentes Virus de Pimiento (Capsicum annuum L. en Especies de Malezas Asociadas al Cultivo Presence of Different Sweet Pepper (Capsicum annuum L. Viruses on Associated Weed Species

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    Juan Ormeño N

    2005-12-01

    Full Text Available En prospecciones de malezas realizadas durante dos temporadas agrícolas, 30 especies anuales y 13 perennes fueron determinadas en cultivos de pimientos (Capsicum annuum L. de los Valles de Elqui y Limarí, Región de Coquimbo (29° a 30º lat. Sur. Los muestreos fueron aleatorios dentro y fuera de los potreros, con y sin cultivo presente, en invierno y primavera. Se recolectaron 676 plantas de malezas, analizándose un total de 379 muestras. Utilizando la prueba DAS-ELISA se determinó Virus del mosaico del pepino (CMV, Virus del bronceado del tomate (TSWV, Virus del mosaico de la alfalfa (AMV, Virus Y de la papa (PVY y Virus INSV (Impatients necrotic spot virus. Un 17% (64 de las muestras fueron positivas al menos a un virus, de las cuales 7,4% (28 provinieron de plantas con síntomas y 9,4% (36 fueron hospederos asintomáticos. Chamico (Datura spp. hospedó a CMV y PVY; nicandra (Nicandra physalodes a AMV, CMV y PVY; tomatillo (Solanum nigrum a CMV y PVY; ñilhue (Sonchus spp. a AMV y TSWV; pacoyuyo (Galinsoga parviflora a AMV, CMV, TSWV y INSV; quingüilla (Chenopodium spp. a AMV, CMV, TSWV, PVY e INSV, entre las principales. Usando un índice potencial de infección relativa (IPIR, los mayores valores se obtuvieron con pacoyuyo (74,7, nicandra (11,2, tomatillo (6,3 y quingüilla (6,0. Especies como ñilhue, chamico, pila-pila (Modiola caroliniana, quilloi-quilloi (Stellaria media y bledo (Amaranthus spp., tuvieron índices inferiores a 1,0. Nicandra y pacoyuyo portaron más de un virus y estas infecciones múltiples prevalecieron sobre las simples. Controlar malezas portadoras tanto dentro como en las inmediaciones de los potreros, resulta imperioso para poder minimizar la incidencia y dispersión de las enfermedades virales en pimientos.In a two growth-cycle survey, 30 annual and 13 perennial weed species were determined in different sweet pepper (Capsicum annuum L. growing areas of the Limari and Elqui valleys of Coquimbo Region of Chile

  2. Acquired severe pneumonia in the community in seropositive HIV patients. Neumonía grave adquirida en la comunidad en paciente seropositiva al virus de inmunodeficiencia humana.

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    Rubén Bembibre Taboada

    Full Text Available Infection due to the Human Immunodeficiency Virus (HIVhas become a principal health problem worldwide mainly in underdeveloped countries. In these patients, respiratory infections constitute the greatest cause of morbimortality rate in which Pneumocystis Carinii is the most frequently found pathogen. However, this article describes the case of a woman who is seropositive to HIV and who developed a severe and rapidly fatal community acquired pneumonia dur to Sthaphylococcus aureus, a very rare and less common infection in this kind of patients.

    La infección por el virus de la inmunodeficiencia humana se ha convertido en el principal problema sanitario en el mundo, particularmente en los países subdesarrollados. En estos pacientes la infección respiratoria es la mayor causa de morbilidad y mortalidad, en cuyos casos el Pneumcystis carinii es el germen más comúnmente encontrado. Sin embargo, en el presente trabajo se describe una paciente seropositiva al virus de la inmunodeficiencia humana que desarrolló una neumonía adquirida en la comunidad, severa y rápidamente fatal, por Sthaphylococcus aureus, infección poco común en este tipo de pacientes.

  3. Perfil de seguridad de los nuevos fármacos frente al virus de la hepatitis C

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    Cárdaba García, María Elena

    2015-01-01

    La infección por el virus de la hepatitis C es un problema de salud de primera magnitud a nivel mundial. Hasta hace poco tiempo, el tratamiento tradicional de esta patología estaba restringido a la terapia combinada con interferón pegilado y ribavirina, y en el caso de que el virus de la hepatitis C fuera genotipo 1, se podían añadir a este esquema boceprevir o telaprevir. Estos esquemas de tratamiento se asocian a numerosas y graves reacciones adversas, que en algunos casos pueden obligar a ...

  4. Infección oculta por el virus de la hepatitis B en hijos de madres positivas al HBsAg

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    Marité Bello-Corredor

    2016-04-01

    Full Text Available La infección oculta por el virus de la hepatitis B (IOB, se caracteriza por la presencia en suero o plasma del genoma viral (ADN-VHB y anticuerpos contra la proteína de la cápside (anti-HBc en ausencia del antígeno de superficie (HBsAg, marcador que tradicionalmente se emplea para identificar la presencia del virus. Con el objetivo de caracterizar la presencia de IOB en hijos de madres positivas al HBsAg, se estudiaron 291 muestras séricas de niños con la condición de ser HBsAg (- y anticuerpos anti-HBsAg (anti-HBs menores de 50 UI/L, conservadas en la seroteca del Laboratorio de Referencia Nacional de Hepatitis Virales. Se realizaron ensayos para determinar la exposición al virus (anti-HBc, a los sueros anti-HBc (+ se les realizó Reacción en Cadena de la Polimerasa en Tiempo Real (RCP-TR para determinar y cuantificar el ADN-VHB. La prevalencia de exposición al VHB (anti-HBc fue 16,8% (49/291. El ADN viral se cuantificó en el 14% (6/43 de los casos anti-HBc (+, observándose cargas virales que oscilaban entre 2,15 x 101 hasta 3,42 x 101 UI/mL. La prevalencia de la IOB para el total de los pacientes analizados fue 2,1% (6/291, considerada relativamente baja. No se encontró asociación significativa entre las variables sociodemográficas analizadas tales como: edad, sexo y provincia de procedencia. La IOB está presente en hijos de madres positivas al HBsAg, a pesar de la profilaxis contra la hepatitis B. Por lo tanto, se requiere de pesquisajes adecuados para detectar dicha entidad. Las implicaciones clínicas y epidemiológicas de la misma, requieren de un estrecho monitoreo y atención de estos pacientes. Este estudio se realiza por primera vez en Cuba y aporta conocimientos útiles para el diagnóstico, prevención y control de esta enfermedad en niños.

  5. Lesiones por virus Herpes simplex y estomatitis aftosa recidivante. Estudio epidemiológico en una muestra elegida al azar

    OpenAIRE

    Puy, D.; Chimenos Küstner, Eduardo; C. Dorado

    1996-01-01

    De entre las lesiones observadas en la cavidad bucal, las producidas por el virus Herpes simplex (VHS) y las originadas en la estomatitis aftosa recidivante (EAR) representan una parte importante de las lesiones que el odontólogo o estomatólogo encuentra cotidianamente. Aunque cada uno de estos dos procesos posee una etiopatogenia y unas características diferentes, en algunas ocasiones su diagnóstico puede llegar a ser controvertido y difícil, debido a su similar apariencia clínica, planteánd...

  6. Capítulo I. Tiempos y espacios huastecos

    OpenAIRE

    Ariel De Vidas, Anath

    2013-01-01

    “...el temple es húmedo y cálido en extremo, en montañas ásperas llenas de fieras [...]; animales ponzoñosos: escorpiones y unas culebras [...] que se dejan caer de los árboles y es mortal su ponzoña [...]. Los mosquitos cubren el sol [...], la sombra de los árboles no sirve, porque todos están de garrapatas cuajados [...]. Con el calor, [la carne] las más veces se llena de gusanos [...]. Muchos de los que entran en aquella región, a pocos días acaban con sus días y, si escapan, quedan con ac...

  7. EVALUACIÓN DE GENOTIPOS DE Lycopersicon esculentum Mill. FRENTE AL VIRUS DEL ENCRESPAMIENTO AMARILLO DE LA HOJA DEL TOMATE (TYLCV

    Directory of Open Access Journals (Sweden)

    F. Dueñas

    2006-01-01

    Full Text Available El virus del encrespamiento amarillo de la hoja del tomate (TYLCV constituye uno de los mayores factores limitantes del cultivo del tomate en Cuba. La obtención de genotipos con niveles de resistencia es una de las soluciones más atractivas para la producción, por la amenaza de esta enfermedad viral para el rendimiento de este cultivo. En el Instituto Nacional de Ciencias Agrícolas (INCA se comenzó un programa de mejora genética de sus variedades comerciales, con la incorporación del gen de resistencia (Ty-1 a TYLCV a partir de la variedad resistente Vyta. Se realizaron cruzamientos entre la variedad resistente Vyta y las variedades susceptibles Amalia, Mariela, Mara, Inifat-28 e Inca-33, a cuya descendencia híbrida, F1, se le hizo el diagnóstico del virus (molecular y no molecular, así como la evaluación agronómica a estos, a los genotipos parentales, al híbrido HA-3019 y a la variedad Campbell-28, estos últimos utilizados como control resistente y susceptible respectivamente. Los resultados del diagnóstico corroboraron el carácter resistente de la variedad Vyta ante la enfermedad, el buen comportamiento de los híbridos derivados de esta así como del híbrido HA 3019, la susceptibilidad de las variedades comerciales y de la variedad Campbell-28.La combinación de los resultados del diagnóstico y las evaluaciones agronómicas permitieron seleccionar a los híbridos Mara x Vyta y Mariela x Vyta como promisorios para la continuación del programa de mejora genética a TYLCV que se desarrolla en el INCA.

  8. Evaluación de accesiones de Capsicum spp. por su reacción al virus del mosaico deformante del pimentón (PepDMV Screening of Capsicum spp. to the deforming mosaic virus from pepper (PepDMV

    Directory of Open Access Journals (Sweden)

    Catherine Pardey R

    2010-01-01

    Full Text Available Se evaluaron 235 accesiones de Capsicum spp. procedentes del Banco de Germoplasma de la Universidad Nacional de Colombia sede Palmira en condiciones de invernadero por su reacción al Virus deformante del Pimentón (PepDMV. Solamente se identificaron 13 accesiones (5% como resistentes al virus, según la ausencia de síntomas y ausencia del virus en pruebas serológicas (PTA-ELISA y RT-PCR. Los materiales resistentes incluyen variedades de C. annuum, C. frutescens, C. chinense y C. baccatum.A total of 235 accessions of Capsicum sp from the gene bank of the Colombian National University campus Palmira’s were screened under controlled glasshouse conditions for their reaction to pepper deforming mosaic virus. Only 5.5 % 8139 of the accessions inoculated showed resistance to the Virus, as determined by symptom expression and serological (PTA-ELISA and RT-PCR. The resistant genotypes included varieties of C. annuum, C frutescens, C. chinense y C. baccatum.

  9. Herencia de la resistencia al virus del mosaico deformante del pimentón PepDMV en Capsicum Inheritance of resistance to the deforming pepper mosaic virus, PepDMV, in Capsicum

    Directory of Open Access Journals (Sweden)

    Mario Augusto García Dávila

    2009-10-01

    Full Text Available En busca de determinar el modo de herencia de la resistencia en tres materiales resistentes, se hicieron cruzamientos hacia tres líneas susceptibles. Se siguió el método del retrocruzamiento propuesto por Warner (1952 fundamentado en el modelo matemático de Fisher Immer y Tedin (1932 descrito con detalle por Mater (1949 y Jenkins (1982, 1984 en el cual se descompone la varianza genética en tres componentes: aditiva, dominancia y epistática. El modelo del retrocruzamiento incluye los dos parentales, el hibrido de la primera generación y la autofecunfación de la F1 para formar la población F2 y las retrocruzas hacia ambos padres. Las poblaciones fueron evaluadas a resistencia al virus del mosaico del pimentón PepDMV en condiciones de invernadero. Los resultados mostraron que el modelo aditivodominancia explicó la resistencia en los híbridos formados entre materiales resistentes y susceptibles. Los genes con acción heredable transmiten a la descendencia el efecto de resistencia. La ganancia de la resistencia viral se da por la presencia de parentales resistentes.With the aim to determine the mode of inheritance in three resistant materials, crosses were performed towards three susceptible lines. We followed the backcrossing method used by Warner (1952, based on the mathematical model of Fisher Immer and tedin (1932, described in detail by Mater (1949 and Jenkins (1982, 1984. In this method the genetic variance is partitioned into three components: additive, dominant and epistatic. The backcross method includes the two parents, the F1 hybrid, and the self-pollination of the F1 to form the F2 population, and the backcrosses to both parents. The populations were evaluated for resistance to the pepper mosaic virusPepDMV in greenhouse conditions. The results showed that the model additive-dominant explained the resistance in the hybrids formed between resistant and susceptible material. The genes with heritable action transmit to their

  10. Heartland Virus

    Science.gov (United States)

    ... Vector-Borne Diseases (DVBD) NCEZID Share Compartir Heartland virus On this Page What is Heartland virus? How ... Do I Need to Know? What is Heartland virus? Heartland virus belongs to a family of viruses ...

  11. Resistencia genética al Virus del Arrugamiento Foliar del Fríjol transmitido por Bemisia tabaci (Hemiptera: Aleyrodidae) Genetic resistance to Bean Leaf Crumple Virus transmitted by Bemisia tabaci (Hemiptera: Aleyrodidae)

    OpenAIRE

    MARÍA ELENA CUÉLLAR J.; Morales, Francisco J.; JAMES MONTOYA LERMA

    2011-01-01

    Desde 2002, los cultivos de fríjol habichuela (Phaseolus vulgaris) de la parte plana del Valle del Cauca han sido afectados por el Virus del Arrugamiento Foliar del Fríjol (Begomovirus: Geminiviridae) agente causal de una nueva y severa enfermedad, transmitido por el biotipo B de la mosca blanca, Bemisia tabaci. Se investigó la relación virus-vector utilizando genotipos de fríjol común seleccionados como posibles fuentes de resistencia. Se determinó la eficiencia de transmisión del virus usan...

  12. Detección de bovinos portadores e inmunotolerantes al virus de la diarrea viral bovina en predios lecheros de la Región Metropolitana de Chile Identification of bovine viral diarrhoea virus in persistently infected immunotolerant cows in herds of the Region Metropolitana, Chile

    Directory of Open Access Journals (Sweden)

    M. CELEDON

    1998-01-01

    Full Text Available Se implementó una técnica para detectar la presencia de bovinos portadores e inmunotolerantes (PIT al virus de la diarrea viral bovina (VDVB, a través de la pesquisa de viremia persistente y bajos títulos de anticuerpos, para el VDVB, en dos muestras de sangre tomadas, desde un mismo animal, con tres meses de separación. La presencia de virus en la sangre se evidenció a través del aislamiento viral (AV -desde muestras de plasma y linfocitos- en cultivos primarios de testículo bovino y la identificación del supuesto aislado se efectuó por prueba de inmunoperoxidasa indirecta (IPI en el segundo pasaje del inóculo sobre las células. La detección de anticuerpos séricos se realizó por prueba de seroneutralización (SN, empleando la cepa citopatogénica Singer del VDVB. Se consideró animal PIT al que evidenció presencia de virus en las 2 muestras de sangre y mantuvo títulos de anticuerpos menores o iguales a 8. De 238 bovinos muestreados en 42 se detectó viremia persistente en ausencia o bajos títulos de anticuerpos, considerándose una prevalencia de 18% de bovinos PIT, y de los 34 predios muestreados en 22 se detectó la presencia de animales PIT, considerándose una prevalencia predial de 65%. Hubo diferencias significativas (p The presence of immunotolerant, persistently infected (IPI animals with bovine viral diarrhoea virus (BVDV was detected in several milking herds with abortions, repeat breeders, still births and poor growth rates, by the virus isolation test and measurement of serum neutralising antibodies, in 2 blood samples from each animal. Samples were taken 3 month apart. Virus isolation was done by inoculation of the plasma and lymphocytes from peripheral blood in primary bovine testicle cell culture. The isolates were identified, on the second serial passage, by the indirect immunoperoxidase test. Cows were considered IPI when BVDV was isolated in the two blood samples and serum title neutralising antibodies were

  13. Estado immune humoral frente al virus del Moquillo canino, el Parvovirus canino y Leptospiras en un criadero (Humoral immune status against Distemper Virus, Canine Parvovirus and Leptospira in a breeding kennel

    Directory of Open Access Journals (Sweden)

    Simón-Valencia MC

    2009-04-01

    Full Text Available ResumenObjetivo: se ha estudiado el estado inmune humoral frente alMoquillo canino (CDV, Parvovirus canino tipo 2 (CPV y Leptospiraspp (LPT en un criadero canino tras la vacunación para detectarposibles factores que puedan influir en la respuesta inmune humoralMaterial y Métodos: es un estudio transversal en 207 animales (80adultos y 127 cachorros de Golden Retriever (GR, LabradorRetriever (LR y Perro de Montaña del Pirineo (PM. Se extrajeronmuestras de suero de los cachorros en 4 momentos diferentes enrelación a la vacunación frente a estos agentes. Los adultos serevacunaban anualmente en Marzo y Abril y se extrajo una solaLa valoración del título de anticuerpos (Ac frente a CDV, CPV y LPTse realizó mediante Seroneutralización, Inhibición de laHemoaglutinación y ELISA respectivamente.Resultados: Los perros adultos mostraron títulos superiores de Acfrente a CPV y LPT e inferiores en Ac frente a CDV que los cachorros(p ≤ 0,05. La raza se asoció a diferencias en los títulos de Ac, siendola raza PM la que mostró los valores más bajos frente a los tresagentes en el conjunto de la población, y estas diferencias fueronSe detectó asociación (p ≤ 0,05 entre el momento de la vacunacióny la media del título alcanzado frente a los tres agentes. La primeravacunación produjo un aumento importante de la media del título deAc (seroconversión, pero posteriores vacunaciones solo produjeronun ligero aumento. Los cachorros GR produjeron la mejor respuestatras la primera vacunación pero solo se detectó asociación con los Acfrente a CPV. Los cachorros mostraron un aumento de la media del título de Ac frente a LPT entre las 6 y 8 semanas de edad sin haber sido vacunados, indicando un posible contacto con leptospiras patógenas en su entorno, además de la posible existencia de roedores, el bajo título de las madres podrían relacionarse con la existencia deportadores renales de leptospiras.SummaryObjective: A breeding kennel was

  14. Presencia de Diferentes Virus de Pimiento (Capsicum annuum L.) en Especies de Malezas Asociadas al Cultivo Presence of Different Sweet Pepper (Capsicum annuum L.) Viruses on Associated Weed Species

    OpenAIRE

    Juan Ormeño N.; Paulina Sepúlveda R

    2005-01-01

    En prospecciones de malezas realizadas durante dos temporadas agrícolas, 30 especies anuales y 13 perennes fueron determinadas en cultivos de pimientos (Capsicum annuum L.) de los Valles de Elqui y Limarí, Región de Coquimbo (29° a 30º lat. Sur). Los muestreos fueron aleatorios dentro y fuera de los potreros, con y sin cultivo presente, en invierno y primavera. Se recolectaron 676 plantas de malezas, analizándose un total de 379 muestras. Utilizando la prueba DAS-ELISA se determinó Virus del ...

  15. Comparison of Molecular (PCR and Pap Smear Methods in Diagnosis of Human Papiloma Virus (HPV in Women with Genit al Warts

    Directory of Open Access Journals (Sweden)

    Bashi zadeh Fakhar, H . (M S c

    2013-09-01

    Full Text Available Background and Objective: Much research has shown that Human Papiloma Virus (HPV plays an important role in cervix cancer and it is the cause of 99% of cervix cancer worldwide. Lots of research has been done to find a proper method for HPV diagnosis and screening in patients with genital warts. This study aimed at comparing PCR method with Pap smear test in HPV screening. Material and Methods: Considering the presence of DNA of HPV, 45 vaginal and cervix swap samples of women with genital warts were tested by means of specific PCR and Pap smear from September 2010 to April 2011. Results: Out of 45 vaginal and cervix swap samples of women suffering genital warts, 37 samples (82.2% are positive. Of 45 Pap smear samples, 13 (29% are neoplasia and 32 (71% normal. Conclusion: The difference between the results of PCR and Pap smear is due to low specification and sensitivity of Pap smear. Thus it is recommended using diagnostic PCR method in addition to Pap smear in order to promote the quality of screening in individuals with genital warts. Keywords: Human Papiloma Virus (HPV; Genital Warts; Molecular (PCR; Pap Smear

  16. ECHO virus

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/001340.htm ECHO virus To use the sharing features on this page, please enable JavaScript. Enteric cytopathic human orphan (ECHO) viruses are a group of viruses that lead to ...

  17. Combining CpG and Al(OH)3 enhances the immunogenicity of hepatitis C virus recombinant ptotein combined vaccine%CpG和Al(OH)3佐剂联合使用增强丙型肝炎病毒重组蛋白疫苗的细胞免疫原性

    Institute of Scientific and Technical Information of China (English)

    李广学; 王国华; 陈坤; 何竟; 曾瑞红; 修冰水; 凌世淦; 张贺秋; 闫涛; 魏林; 贺峰; 冯晓燕; 宋晓国

    2010-01-01

    目的 CpG和Al(OH)3佐剂联合使用增强丙型肝炎病毒(HCV)重组蛋白联合疫苗(TFE)的细胞免疫原性.方法 以CpG、Al(OH)3、Al(OH)3+CpG和弗氏佐剂(FA)为免疫佐剂,分别与TFE混合免疫BALB/c小鼠.末次免疫后10 d取静脉血,分离血清,用ELISA方法测定血清中特异性抗体,并每组处死5只小鼠,分离小鼠脾细胞,体外检测IFN-γ、IL-4和CTL杀伤试验;剩余的小鼠背部皮下注射1×106个SP2/O-NS3细胞,观察其保护作用.组间均数差异用LSD-t检验.结果 TFE+Al(OH)3+CpG组的特异性CTL杀伤能力高于TFE+FA组和TFE+CpG组(P<0.05);与TFE+Al(OH)3组和TFE+CpG组相比,TFE+Al(OH)3+CpG组小鼠脾细胞中分泌IFN-γ的淋巴细胞的数量显著增多(P<0.05).结论 Al(OH)3和CpG合用能显著增强HCV重组蛋白联合疫苗TFE的细胞免疫原性;TFE+Al(OH)3+CpG能有效预防表达HCV非结构蛋白NS3的细胞SP2/0-NS3对免疫小鼠的攻击.%Objective To research CpG and Al(OH)3 adjuvants enhancing immunogenicity of hepatitis C virus(HCV) recombinant ptotein combined vaccine(TIE).Methods BALB/c mice were immunized with candidate vaccine TFE using CpG,Al(OH)3,Al(OH) 3 + CpG,or freund's adjuvant(FA) as the adjuvant.Five mice were sacrificed after 10 d of the last immunization.Specific antibodies in sera were tested by enzyme-linked immunosorbent assay(ELISA).Splenic cells were isolated and levels of IFN-γ,IL-4 and cytotoxic T lymphocyte(CTL) cytotoxicity assay were messuredin vitro.The remaining mice were subcutaneouly injected with 1 × 106 SP2/0-NS3 cells on the back to investigate the protective effects.The differences of means between groups were compared by LSD-t test.Results The specific CTL activity of TFE + A1(OH) 3 + CpG group was higher than TFE + FA group and TFE + CpG group(P < 0.05).The level of IFN-γsecreting cells in TFE + Al(OH)3 + CpG group was higher than that in TFE + M(OH)3 group or TFE + CpG group(P < 0.05).Conclusion Combining Al(OH) 3 and CpG could enhance

  18. The Virus Business

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The Panda, a computer infection that wreaked havoc on computers across China, brought attention to the country’s underground computer virus business, but it was just the tip of a growing iceberg The cute and cuddly panda, a nation- al treasure of China

  19. Cobertura e inmunorespuesta a la vacunación frente al virus de la hepatitis B en adolescentes de la provincia de Guadalajara

    Directory of Open Access Journals (Sweden)

    Martínez Pérez José A.

    1998-01-01

    Full Text Available FUNDAMENTO. La hepatitis B constituye uno de los mayores problemas de salud a nivel mundial. Una de las medidas para su prevención es la vacunación masiva de los adolescentes. El objetivo de nuestro estudio fue evaluar la cobertura del programa de vacunación sistemática antihepatitis B entre la población de 8º curso de EGB de la provincia de Guadalajara y estudiar la inmunorrespuesta de la misma, relacionándola con otras variables. MÉTODOS. Se trata de un estudio transversal. La cobertura del programa vacunal se estudió en toda la población diana de la provincia. Para el estudio de la inmunorrespuesta se obtuvo una muestra de 338 alumnos, mediante muestreo aleatorio sistemático. Se cuantificaron los títulos de anti-HBs al 6º mes de la tercera dosis, considerándose niveles protectores los iguales o superiores a 10 mU/ml. RESULTADOS. El porcentaje de personas correctamente vacunadas fue del 82,7%. La tasa de seroprotección global fue del 97,5%. El 46,7% de la muestra respondieron con títulos superiores a 1000 mU/ml. Existió relación inversa estadísticamente significativa (p<0,001 entre el nivel de anti-HBs y el de Quetelet. No encontramos diferencias significativas entre los dos sexos al comparar los títulos postvacunales de anti-Hbs CONCLUSIONES. Estimamos satisfactoria la cobertura alcanzada y buena la inmunogenicidad de la vacuna. La obesidad es un factor predictor de baja respuesta a la misma. Esta respuesta no se ve influenciada por el sexo.

  20. Herramientas biotecnológicas aplicadas al estudio del cáncer: el modelo de cáncer de cuello uterino y su relación con el virus del papiloma humano (hpv

    Directory of Open Access Journals (Sweden)

    Orozco O.

    1999-12-01

    Full Text Available El Cáncer de cuello uterino es un problema de salud pública mundial, con más de 500.000 nuevos casos al año y 200.000 muertes, 80% de los cuales ocurren en países del tercer mundo. Las evidencias morfológicas, epidemiológicas y moleculares indican que la infección por el virus del Papiloma Humano (HPV está fuertemente asociada con el desarrollo del tumor. El estudio del papel del virus es importante tanto desde el punto de vista de la comprensión del proceso carcinogénico en sí, como desde la perspectiva de la implementación de medidas que previniendo o controlando la infección viral, puedan llevar a evitar la aparición del cáncer y/o controlar su crecimiento. Hasta hace poco, estos estudios se vieron limitados por la dificultad de obtener material viral suficiente, ya sea de las lesiones o por medio de cultivo «in vitro» de células infectadas. Los avances recientes en las técnicas inmunológicas, de ingeniería genética y de síntesis química han permitido aplicar una perspectiva biotecnológica en este modelo, lográndose un conocimiento muy importante de los mecanismos carcinogénicos vírales y de la interacción virus-huésped en el ámbito de la respuesta inmune. Lo anterior ha permitido proponer por ejemplo la vacunación con fines profilácticos (evitar la infección viral primaria y por ende la aparición del tumor o con fines terapéuticos (eliminación inmunológica de células ya infectadas y tumorales. En nuestro laboratorio hemos empleado estas técnicas para producir anticuerpos monoclonales anti-péptidos sintéticos derivados de la secuencia de la oncoproteína E7 de HPV 16, para buscar con esta herramienta la caracterización de la protema nativa, su detección en tejidos tumorales y entender mejor su papel en el proceso oncogénico. También hemos logrado introducir el gen de esta proteína en un vector plasmídico, expresando posteriormente el producto en bacterias y obteniendo así el material

  1. Virus en Endodoncia

    OpenAIRE

    Hernández Vigueras, Scarlette; Salazar Navarrete, Luis; Pérez Tomás, Ricardo E.; Segura Egea, Juan José; Viñas, Miquel; López López, José

    2014-01-01

    La infección endodóntica es la infección que afecta al sistema de conductos radiculares y, sin duda, es el principal agente etiológico de las periodontitis apicales. Además, de las bacterias patógenas endodónticas, se ha buscado en los últimos años asociar la presencia de virus en distintos tipos de patología endodóntica. Los virus que más se han buscado y asociado son los pertenecientes a la familia herpesvirus, los cuales se han encontrado presentes en patologías periapicales principalmente...

  2. Chikungunya Virus

    Science.gov (United States)

    ... Gaines, PhD, MPH, MA, CHES Differentiating Chikungunya From Dengue: A Clinical Challenge For Travelers CDC Travelers' Health Chikungunya Virus Home Prevention Transmission Symptoms & Treatment Geographic Distribution Chikungunya virus in ...

  3. Zika Virus

    Science.gov (United States)

    Zika is a virus that is spread mostly by mosquitoes. A pregnant mother can pass it to ... through blood transfusions. There have been outbreaks of Zika virus in the United States, Africa, Southeast Asia, ...

  4. Chikungunya virus

    Science.gov (United States)

    Chikungunya virus infection; Chikungunya ... Where Chikungunya is found Before 2013, the virus was found in Africa, Asia, Europe, and the Indian and Pacific oceans. In late 2013, outbreaks occurred for the first time in the ...

  5. Acute paralysis viruses of the honey bee

    Institute of Scientific and Technical Information of China (English)

    Chunsheng; Hou; Nor; Chejanovsky

    2014-01-01

    <正>The alarming decline of honey bee(Apis mellifera)colonies in the last decade drove the attention and research to several pathogens of the honey bee including viruses.Viruses challenge the development of healthy and robust colonies since they manage to prevail in an asymptomatic mode and reemerge in acute infections following external stresses,as well as they are able to infect new healthy colonies(de Miranda J R,et al.,2010a;de Miranda J R,et al.,2010b;Di Prisco G,et al.,2013;Nazzi F,et al.,2012;Yang X L,et al.,2005).

  6. ECHO virus

    Science.gov (United States)

    Enteric cytopathic human orphan (ECHO) viruses are a group of viruses that lead to gastrointestinal infection and skin rashes. ... Echovirus is one of several families of viruses that affect the ... are common. In the United States, they are most common in ...

  7. Zika Virus

    Science.gov (United States)

    ... Zika Virus | See Q&A —June 21, 2016 Zika Virus Protein Could Be Vaccine Target —May 19, 2016 Research Conducted and Supported by the National Institutes of Health (NIH) in Addressing Zika Virus Disease. Testimony before the House Democratic Steering ...

  8. ALS Association

    Science.gov (United States)

    ... toward a world without ALS! Walk to Defeat ALS® Walk to Defeat ALS® draws people of all ... We need your help. I Will Advocate National ALS Registry The National ALS Registry is a congressionally ...

  9. New insights of Sacbrood virus

    Institute of Scientific and Technical Information of China (English)

    Mingxiao; Ma

    2014-01-01

    <正>Over the last several years,major efforts have been expended to study viral infection of honeybees mainly due to colony losses around the world(Allen M,et al.,1996).It seems that honeybees are infected with numerous viruses mounting to 18so far.Infection may be asymptomatic but could still have adverse effects on the bee and may even cause death resulting in colony collapse.Sacbrood virus(SBV)is the most widely distributed of all honey bee viruses.

  10. Seroprevalencia de infección por virus C de la hepatitis en población reclusa del noroeste de España al ingreso en prisión

    Directory of Open Access Journals (Sweden)

    1998-01-01

    Full Text Available FUNDAMENTO: Las prisiones españolas albergan un elevado número de personas con prácticas de riesgo para la infección por virus de la hepatitis C (VHC. El objeto de este trabajo es conocer la prevalencia de esta infección en la población reclusa del noroeste de España y sus factores asociados. MÉTODOS: Se estudia a las personas ingresadas en siete centros penitenciarios del noroeste español. Se recogieron variables socio-demográficas, penitenciarias y factores de riesgo para la infección por VHC. Se realizó determinación de anticuerpos frente a VHC (EIA e INNO-LIA HCV III, virus de la hepatitis B (VHB (EIA y virus de la inmunodeficiencia humana (VIH (EIA y Western-Blott. El análisis de los factores asociados se basó en regresión logística. RESULTADOS: El 47,9% de los estudiados presentaron anticuerpos frente a VHC. Destacó la mayor prevalencia en: UDVP (89,6%, los que compartieron jeringuillas (94%, los infectados por VIH (92,7%, los portadores de antígeno Australia (65,1% y de anticuerpos frente al antígeno del core del VHB (79,8%, aquellos con ingresos previos en prisión (60,9 %, los solteros (54,8 %, los gitanos (52 %, los trabajadores no cualificados (50,4 %, los que no habían obtenido el graduado escolar (50,9 %, los tatuados (66,7 % y aquellos con antecedentes de autolesiones (79,3 %. En el análisis de regresión logística las variables asociadas a la infección por VHC fueron: UDVP (OR=33,3; I.C.95%=25-50, HBcAc (+ (OR=4,1; I.C.95%=2,9-6,3, no graduado escolar (OR=1,6; I.C.95%=1,1-2,5, infección por VIH (OR=2,4; I.C.95%=1,1-5,3, edad (OR=0,98; I.C.95%=0,96-1,00, meses en prisión (OR=1,011;I.C.95%=1,004-1,019 y antecedentes de estancias previas en prisión (OR= 2,3; I.C.95%=1,5-3,6. CONCLUSIONES: La prevalencia de infección por VHC en presos es muy elevada. Se aconseja potenciar las medidas preventivas (programas de reducción del daño y la elaboración de protocolos de seguimiento clínico-analítico de los

  11. DEFORMED WING VIRUS IN Apis mellifera L.: PREVALENCE, MORPHOLOGY, AND PATHOGENICITY (review)

    OpenAIRE

    V.E. VOLYKHINA

    2015-01-01

    Viral infections are not considered the most dangerous honeybee (Apis mellifera L.) diseases though being rather harmful. Virus-caused honeybee pathologies are mainly symptomless (N.J. Dimmock et al., 1987; A.C.F. Hung et al., 1996), nevertheless, a rapid replication of the viruses can be triggered leading to clinical manifestation and even death of the insects (R. Singh et al., 2010). In honeybee families a simultaneous circulation of several viruses can occur. Acute bee paralysis virus (ABP...

  12. Witwer, K.W.; et al. Correction: miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction. Viruses 2012, 4, 1844-1864

    OpenAIRE

    Witwer, Kenneth W.; Clements, Janice E.; Sisk, Jeanne M.

    2013-01-01

    A funding designation in our 2012 Viruses publication, doi: 10.3390/v4101844 (Viruses 2012, 4, 1844-1864), contained an incorrect digit. On page 1860, (Acknowledgments), U19 support was incorrectly listed as AI076113. The correct designation is AI096113.

  13. Witwer, K.W.; et al. Correction: miRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction. Viruses 2012, 4, 1844-1864.

    Directory of Open Access Journals (Sweden)

    Kenneth W. Witwer

    2013-03-01

    Full Text Available A funding designation in our 2012 Viruses publication, doi: 10.3390/v4101844 (Viruses 2012, 4, 1844-1864, contained an incorrect digit. On page 1860, (Acknowledgments, U19 support was incorrectly listed as AI076113. The correct designation is AI096113.

  14. Computer Virus

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Computer viruses are small software programs that are designed to spread from one computerto another and to interfere with computer operation.A virus might delete data on your computer,use your e-mail program to spread itself to othercomputers,or even erase everything on your hard disk.Viruses are most easily spread by attach-ments in e-mail messages or instant messaging messages.That is why it is essential that you never

  15. Medicina tradicional-medicina moderna entre los huastecos de San Luis Potosí

    OpenAIRE

    Gallardo Arias, Patricia

    2010-01-01

    Este trabajo tiene como objetivo describir y analizar las relaciones que mantienen los teenek y nahuas de la Huasteca potosina con los diferentes programas gubernamentales e institucionales para la salud. Con lo anterior debe quedar claro que la medicina tradicional forma parte de una resistencia que se da entre la cultura indígena y la cultura mestiza. Dicha resistencia se entiende porque la medicina hegemónica mantiene como proyecto único ser prioritaria para toda la población en un mismo t...

  16. Archaeal viruses of the sulfolobales

    DEFF Research Database (Denmark)

    Erdmann, Susanne; Garrett, Roger Antony

    2015-01-01

    Infection of archaea with phylogenetically diverse single viruses, performed in different laboratories, has failed to activate spacer acquisition into host CRISPR loci. The first successful uptake of archaeal de novo spacers was observed on infection of Sulfolobus solfataricus P2 with an environm......Infection of archaea with phylogenetically diverse single viruses, performed in different laboratories, has failed to activate spacer acquisition into host CRISPR loci. The first successful uptake of archaeal de novo spacers was observed on infection of Sulfolobus solfataricus P2 with an...... CRISPR loci of Sulfolobus species from a second coinfecting conjugative plasmid or virus (Erdmann and Garrett, Mol Microbiol 85:1044-1056, 2012; Erdmann et al. Mol Microbiol 91:900-917, 2014). Here we describe, firstly, the isolation of archaeal virus mixtures from terrestrial hot springs and the...

  17. Method for detecting viruses in aerosols.

    Science.gov (United States)

    Wallis, C; Melnick, J L; Rao, V C; Sox, T E

    1985-11-01

    A simple method with poliovirus as the model was developed for recovering human enteric viruses from aerosols. Filterite filters (pore size, 0.45 micron; Filterite Corp., Timonium, Md.) moistened with glycine buffer (pH 3.5) were used for adsorbing the aerosolized virus. No virus passed the filter, even with air flow rates of 100 liters/min. Virus recovery from the filter was achieved by rapid elution with 800 ml of glycine buffer, pH 10. The virus in the primary eluate was reconcentrated by adjusting the pH to 3.5, adding AlCl3 to 0.0005 M, collecting the virus on a 0.25-micron-pore Filerite disk (diameter, 25 mm) and and eluting with 6 ml of buffer, pH 10. With this method, virus could be detected regularly in aerosols produced by flushing when 3 X 10(8) PFU of poliovirus were present in the toilet bowl. Poliovirus-containing fecal material from two of four infants who had recently received oral polio vaccine also yielded virus in the aerosols when feces containing 2.4 X 10(7) to 4.5 X 10(7) PFU of virus had been added to the toilet bowl. Persons infected with a variety of natural enteric viruses are known to excrete this amount of virus in their daily stools. PMID:3004329

  18. Viruses, definitions and reality

    Directory of Open Access Journals (Sweden)

    Libia Herrero-Uribe

    2011-09-01

    de la red de la vida y que actúan como meros artefactos mecánicos a la hora de intercambiar genes entre los diferentes organismos. Este artículo revisa varias definiciones de organismos vivos para determinar si los virus se ajustan a ellas, y adicionalmente, considera los nuevos descubrimientos que retan las definiciones actuales de los virus. La fábricas de virus son discutidas ya que estas mini-organelas son un buen ejemplo de la complejidad de las infecciones virales, no como una usurpación mecánica de las estructuras de la célula, pero como una fuerza vital que lleva a la reorganización y la modificación de las estructuras de las células por enzimas celulares y virales. Los nuevos descubrimientos como los Mimivirus, su virófago y virus que producen colas filamentosas cuando se encuentran fuera de la célula, han estimulado a la comunidad científica a analizar la definición actual de los virus. Para la innovación se debe estar libre de estructuras mentales rígidas o apegadas al pasado, para lograr comprender e integrar los nuevos descubrimientos que traerán las investigaciones futuras. Los procesos de la vida deben verse desde la perspectiva de la complejidad y la trans-disciplinariedad que incluye y acepta la temporalidad de los procesos activos de los organismos vivos y su interdependencia e interrelación entre ellos y su ambiente.

  19. Uso de péptidos de la proteína E2 del virus de la hepatitis G para inhibir la actividad del virus VIH

    OpenAIRE

    Gómara Elena, María José; Haro Villar, Isabel

    2009-01-01

    Uso de péptidos de la proteína E2 del virus de la hepatitis G para inhibir la actividad del virus VIH. La presente invención se refiere al uso de al menos un péptido, o derivado del mismo, contenido en la secuencia de la proteína E2 del virus de la hepatitis G (GBVC/ HGV), capaz de inhibir la actividad del virus VIH, impidiendo o reprimiendo la función y/o actividad del virus VIH (Virus de Inmunodeficiencia Humana), de forma transitoria o permanente, o además capaz de ...

  20. Uso de péptidos de la proteína E1 del virus de la hepatitis G para inhibir la actividad del virus VIH

    OpenAIRE

    Gómara Elena, María José; Haro Villar, Isabel

    2009-01-01

    Uso de péptidos de la proteína E1 del virus de la hepatitis G para inhibir la actividad del virus VIH. La presente invención se refiere al uso de al menos un péptido, o derivado del mismo, contenido en la secuencia de la proteína E1 del virus de la hepatitis G (GBV-C/HGV), capaz de inhibir la actividad del virus VIH, impidiendo o reprimiendo la función y/o actividad del virus VIH (Virus de Inmunodeficiencia Humana), de forma transitoria o permanente, o además capaz de ...

  1. Computer viruses

    Science.gov (United States)

    Denning, Peter J.

    1988-01-01

    The worm, Trojan horse, bacterium, and virus are destructive programs that attack information stored in a computer's memory. Virus programs, which propagate by incorporating copies of themselves into other programs, are a growing menace in the late-1980s world of unprotected, networked workstations and personal computers. Limited immunity is offered by memory protection hardware, digitally authenticated object programs,and antibody programs that kill specific viruses. Additional immunity can be gained from the practice of digital hygiene, primarily the refusal to use software from untrusted sources. Full immunity requires attention in a social dimension, the accountability of programmers.

  2. Zika Virus.

    Science.gov (United States)

    Phillips, Jennan A; Neyland, Anavernyel

    2016-08-01

    Zika virus (ZIKV) infections are the latest global public health emergency. Occupational health nurses can protect society by educating workers, women of childbearing age, and others traveling in ZIKV-infected areas about prevention strategies. PMID:27411846

  3. Correction: Graillot, B.; et al. Progressive Adaptation of a CpGV Isolate to Codling Moth Populations Resistant to CpGV-M. Viruses 2014, 6, 5135–5144

    Directory of Open Access Journals (Sweden)

    Benoît Graillot

    2015-12-01

    Full Text Available In our article “Progressive Adaptation of a CpGV Isolate to Codling Moth Populations Resistant to CpGV-M.” (Viruses 2014, 6, 5135–5144; doi:10.3390/v6125135 [1] we obtained resistance values of the codling moth, Cydia pomonella, RGV laboratory colony [2], when challenged with Cydia pomonella Granulovirus, Mexican Isolate (CpGV-M, that were lower than those previously published [2]. Careful analysis of both the RGV colony and the CpGV-M virus stock used led to the realization that a low level contamination of this virus stock with CpGV-R5 occurred. We have made new tests with a verified stock, and the results are now in agreement with those previously published.

  4. Live Virus Smallpox Vaccine

    Science.gov (United States)

    ... A - Z Index SMALLPOX FACT SHEET The Live Virus Smallpox Vaccine The vaccinia virus is the "live ... it cannot cause smallpox. What is a "live virus" vaccine? A "live virus" vaccine is a vaccine ...

  5. El virus del zika

    OpenAIRE

    Sáez García, Cristina

    2016-01-01

    El propósito de este trabajo es hacer una revisión sobre los datos conocidos actualmente relacionados con la infección por el virus Zika y sus consecuencias a nivel neurológico en el desarrollo fetal. La prevención se vuelve esencial contra esta enfermedad: Al no existir vacuna disponible, la actuación debe centrarse en evitar la picadura del mosquito, especialmente aquellas mujeres embarazadas. Las cifras actuales de infectados, y las de bebés nacidos con microcefalia resultan...

  6. Risk behavior for the Human Immunodeficiency Virus among motel clients Las conductas de riesgo al virus de inmunodeficiencia humana entre usuarios de moteles Comportamentos de risco frente ao vírus da imunodeficiência humana entre frequentadores de motéis

    Directory of Open Access Journals (Sweden)

    Helisamara Mota Guedes

    2012-06-01

    Full Text Available This study aimed to verify risk behavior for infection with the Human Immunodeficiency Virus in people who attended motels. This is a cross-sectional study conducted in two motels in two municipalities in the state of Minas Gerais, Brazil, with a sample of 308 randomly selected individuals, aged between 18 and 60 years. Bivariate (chi-square and t test for independent samples and multivariate (logistic regression statistical tests were performed. A total of 45.8% of the participants reported not having used a condom during the last vaginal sexual intercourse, 48.4% did not use a condom during the last oral sex, while 26.3% reported not having used a condom in the last anal intercourse. Having a steady partner was the strongest predictor of not using condoms. Probable beliefs regarding loyalty involved in a stable relationship may be contributing to the failure to use condoms during intercourse, increasing the risk of HIV infection.Este estudio tuvo como objetivo determinar las conductas de riesgo al virus de inmunodeficiencia humana en personas que asistieron moteles. Estudio transversal realizado en dos moteles en dos municipios de Minas Gerais con una muestra de 308 personas. Bivariado se realizaron pruebas estadísticas (Jue-cuadrado y t para muestras independientes y multivariado (regresión logística. La mayoría (42,5% tenían entre 18 y 25 años y el 45,8% no había utilizado preservativo en sus últimas relaciones sexuales vaginales, 48,4% no usó condón para sexo oral, mientras que 26,3 % reportó no haber utilizado el su última relación anal. Tener una pareja estable fue el mejor predictor para no usarse condones. Llegamos a la conclusión de que la lealtad tiende a estar involucrada en una relación estable puede estar contribuyendo a la no utilización de preservativos en las relaciones, aumentando la vulnerabilidad.Este estudo teve por objetivo verificar comportamentos de risco referentes à infecção pelo vírus da imunodefici

  7. Respiratory Syncytial Virus Infections in Infants: Detel1ninants of Clinical Severity

    OpenAIRE

    Brandenburg, Afke

    2000-01-01

    textabstractIn 1955 a virus was isolated by Morris et al. from a chimpanzee with an upper respiratory tract infection. This apparently new virus was originally called chimpanzee coryza agent. Soon aftclwards, when it was isolated from children with respiratory disease, it became clear that this virus was a major human pathogen. The virus was from then onward called respiratory syncytial virus (RSV) because of its ability to caLise respiratory disease and to induce large syncytia in cell cultu...

  8. Computer Viruses. Technology Update.

    Science.gov (United States)

    Ponder, Tim, Comp.; Ropog, Marty, Comp.; Keating, Joseph, Comp.

    This document provides general information on computer viruses, how to help protect a computer network from them, measures to take if a computer becomes infected. Highlights include the origins of computer viruses; virus contraction; a description of some common virus types (File Virus, Boot Sector/Partition Table Viruses, Trojan Horses, and…

  9. Ebola Virus

    Directory of Open Access Journals (Sweden)

    Anusha Rangare Lakshman

    2015-09-01

    Full Text Available The disease Ebola takes its name from the Ebola River situated near a village in the Democratic Republic of Congo, where the disease first appeared in 1976. It is caused by a virus from the Filoviridae family (filovirus. The present outbreak of Ebola Virus Disease (EVD concerns four countries in West Africa, namely Guinea, Liberia, Sierra Leone and Nigeria till date. Further to widespread transmission of the disease, it has been declared as a Public Health Emergency of International Concern by the World Health Organisation on 8 August 2014. As of 4 August 2014, countries have reported 1,711 cases (1,070 confirmed, 436 probable, 205 suspect, including 932 deaths. This review paper enlightens about the awareness of Ebola virus and its preventive measures. [Archives Medical Review Journal 2015; 24(3.000: 296-305

  10. Cats as a potential source of emerging influenza virus infections

    Institute of Scientific and Technical Information of China (English)

    Taisuke; Horimoto; Fumihiro; Gen; Shin; Murakami; Kiyoko; Iwatsuki-Horimoto; Kentaro; Kato; Masaharu; Hisasue; Masahiro; Sakaguchi; Chairul; A.; Nidom; Yoshihiro; Kawaoka

    2015-01-01

    <正>Dear Editor,Historically,the influenza virus has not been regarded as a major pathogen of cats.However,since 2003,natural infections of domestic cats with highly pathogenic H5N1 avian virus causing fatal cases have been reported(Songserm et al.,2006;Yingst et al.,2006;Klopfleisch et al.,2007).Furthermore,infections of this animal with A(H1N1)pdm09 virus,causing respiratory illness with some fatal cases,have also been reported in various parts

  11. Passatempo Virus, a Vaccinia Virus Strain, Brazil

    OpenAIRE

    Leite, Juliana A.; Drumond, Betânia P.; Trindade, Giliane S; Zélia I P Lobato; da Fonseca, Flávio G.; dos Santos, João R.; Madureira, Marieta C.; Guedes, Maria I.M.C.; Ferreira, Jaqueline M. S.; Bonjardim, Cláudio A.; Ferreira, Paulo C. P.; Kroon, Erna G.

    2005-01-01

    Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.

  12. Oropuche virus: A virus present but ignored

    Directory of Open Access Journals (Sweden)

    Salim Mattar V.

    2015-09-01

    Full Text Available Bunyaviruses are RNA viruses that affect animals and plants; they have five genera and four of them affect humans: Orthobunyavirus, Nairovirus, Phlebovirus and Hantavirus. All of them are Arbovirus, except Hantavirus. The Orthobunyaviruses comprise Oropouche, Tahyna, La Crosse virus, California encephalitis virus and Heartland virus recently discovered (1. Except for Heartland virus which is transmitted by ticks of the genus Amblyoma, these Phleboviruses have as vectors mosquitoes, which bite small mammals which are able to be as reservoirs amplifiers.

  13. Plant Virus Metagenomics: Advances in Virus Discovery.

    Science.gov (United States)

    Roossinck, Marilyn J; Martin, Darren P; Roumagnac, Philippe

    2015-06-01

    In recent years plant viruses have been detected from many environments, including domestic and wild plants and interfaces between these systems-aquatic sources, feces of various animals, and insects. A variety of methods have been employed to study plant virus biodiversity, including enrichment for virus-like particles or virus-specific RNA or DNA, or the extraction of total nucleic acids, followed by next-generation deep sequencing and bioinformatic analyses. All of the methods have some shortcomings, but taken together these studies reveal our surprising lack of knowledge about plant viruses and point to the need for more comprehensive studies. In addition, many new viruses have been discovered, with most virus infections in wild plants appearing asymptomatic, suggesting that virus disease may be a byproduct of domestication. For plant pathologists these studies are providing useful tools to detect viruses, and perhaps to predict future problems that could threaten cultivated plants. PMID:26056847

  14. Evolución de la prevalencia de infección por virus de la inmunodeficiencia humana en población reclusa al ingreso en prisión durante el período 1991-1995

    OpenAIRE

    Martín Vicente; Caylà Joan A.; Morís María L.; Alonso Luis E.; Pérez Rafael

    1997-01-01

    FUNDAMENTO: Las prisiones españolas albergan un elevado número de usuarios de drogas por vía parenteral (UDVP), y otras personas con prácticas de riesgo para la infección por virus de la inmunodeficiencia humana (VIH). El objetivo de este trabajo es conocer la evolución de la prevalencia de infección por VIH en el momento del ingreso en prisión y los factores asociados a la misma en este colectivo, lo que puede permitir una aproximación a la efectividad de las estrategias de reducción de ries...

  15. Das Usutu-Virus als Ursache von Massensterben bei Amseln Turdus merula und anderen Vogelarten in Europa: Erfahrungen aus fünf Ausbrüchen zwischen 2001 und 2011

    OpenAIRE

    Bosch, Stefan; Schmidt-Chanasit, Jonas; Fiedler, Wolfgang

    2012-01-01

    Das Usutu-Virus (USUV) ist ein durch Stechmücken der Gattung Culex übertragenes Flavivirus. Es wurde wahrscheinlich durch Zugvögel oder Stechmücken nach Europa eingeschleppt und hat sich inzwischen dauerhaft in der einheimischen Stechmücken-und Vogelfauna etabliert. Seit seinem Erstnachweis 2001 in Österreich hat das USUV fünf größere Ausbrüche mit USUV-assoziierten Vogelsterben, vor allem unter Amseln und in Volieren gehaltenen Eulen, sowie vermutlich kleinere Ausbrüche ohne augenfälliges Vo...

  16. Producción de proteínas recombinantes de valor inmunogénico del virus de la leucosis bovina y su aplicación al diagnóstico de la enfermedad

    OpenAIRE

    Larsen, Alejandra

    2014-01-01

    El virus de la leucosis bovina (VLB) es el agente causal de la Leucosis Enzoótica Bovina (LEB), enfermedad de distribución mundial, presente en nuestro país. En nuestro país los niveles de prevalencia oscilan entre 33% a 84% dependiendo del tipo de explotación bovina, estos altos valores de prevalencia se traducen en grandes pérdidas económicas incluyendo la perdida de mercados internacionales. El programa nacional de control y erradicación reglamentado por el Servicio Nacional de Sanidad Ani...

  17. Zika Virus.

    Science.gov (United States)

    Musso, Didier; Gubler, Duane J

    2016-07-01

    Zika virus (ZIKV) is an arthropod-borne virus (arbovirus) in the genus Flavivirus and the family Flaviviridae. ZIKV was first isolated from a nonhuman primate in 1947 and from mosquitoes in 1948 in Africa, and ZIKV infections in humans were sporadic for half a century before emerging in the Pacific and the Americas. ZIKV is usually transmitted by the bite of infected mosquitoes. The clinical presentation of Zika fever is nonspecific and can be misdiagnosed as other infectious diseases, especially those due to arboviruses such as dengue and chikungunya. ZIKV infection was associated with only mild illness prior to the large French Polynesian outbreak in 2013 and 2014, when severe neurological complications were reported, and the emergence in Brazil of a dramatic increase in severe congenital malformations (microcephaly) suspected to be associated with ZIKV. Laboratory diagnosis of Zika fever relies on virus isolation or detection of ZIKV-specific RNA. Serological diagnosis is complicated by cross-reactivity among members of the Flavivirus genus. The adaptation of ZIKV to an urban cycle involving humans and domestic mosquito vectors in tropical areas where dengue is endemic suggests that the incidence of ZIKV infections may be underestimated. There is a high potential for ZIKV emergence in urban centers in the tropics that are infested with competent mosquito vectors such as Aedes aegypti and Aedes albopictus. PMID:27029595

  18. Computer Viruses: An Overview.

    Science.gov (United States)

    Marmion, Dan

    1990-01-01

    Discusses the early history and current proliferation of computer viruses that occur on Macintosh and DOS personal computers, mentions virus detection programs, and offers suggestions for how libraries can protect themselves and their users from damage by computer viruses. (LRW)

  19. Understanding How Zika Virus Enters and Infects Neural Target Cells.

    Science.gov (United States)

    Miner, Jonathan J; Diamond, Michael S

    2016-05-01

    Zika virus is a mosquito-transmitted flavivirus that has become a public health concern because of its ability to cause microcephaly. In this issue of Cell Stem Cell, Tang et al. (2016) and Nowakowski et al. (2016) use human neural stem cell models and single-cell RNA sequencing to investigate Zika virus tropism and potential entry receptors. PMID:27152436

  20. La sustitución eucalórica de triglicéridos de cadena larga por triglicéridos de cadena media mejora la composición corporal y el perfil lipídico en un paciente con lipodistrofia asociada al virus de la inmunodeficiencia humana Eucaloric substitution of medium chain triglycerides for dietary long chain fatty acids improves body composition and lipid profile in a patient with human immunodeficiency virus lipodystrophy

    Directory of Open Access Journals (Sweden)

    C. Vázquez

    2006-08-01

    Full Text Available Antecedentes: La lipodistrofia asociada a la infección por el virus de la inmunodeficiencia humana (VIH es una patología frecuente, caracterizada por una pérdida de tejido adiposo en las extremidades, región glútea y cara, junto con un depósito excesivo del mismo en el cuello y abdomen. La coexistencia de dislipemia y diabetes mellitus en la lipodistrofia asociada al VIH es también frecuente, y aumenta el riesgo cardiovascular de los pacientes que la padecen. Aunque hay tratamientos disponibles para las alteraciones metabólicas asociadas, hasta la fecha no existen terapias con un claro beneficio demostrado para la lipodistrofia. Caso clínico: Un paciente de 42 años con lipodistrofia asociada al VIH fue inicialmente tratado con dieta hipocalórica (Background: Lipodystrophy is a frequent disorder among patients with human immunodeficiency virus (HIV infection, characterized by a loss of adipose tissue from the extremities, gluteal region and face, with excess fat in the neck and abdominal region. Metabolic abnormalities such as hyperlipidaemia and diabetes mellitus frequently coexist, posing these patients to an increased cardiovascular risk. Drug therapy may improve some of these metabolic disturbances, but to date there are no treatments for lipodystrophy with proven benefit. Case report: A 42 year old man with HIV lipodystrophy was started on a standard low caloric diet with <30% of total fat and < 10% of saturated fat, together with rosiglitazone 8 mg daily. After five months of treatment, given that lipodystrophic features and dyslipidaemia were still present in our patient, we tried to further improve therapeutic results by eucaloric substitution of medium chain triglycerides for dietary long chain fatty acids. Three months later, a dramatic change in body composition was shown with an increase in lean mass and a decrease in fat mass, together with an improvement in lipid profile. Conclusion: Eucaloric substitution of medium chain

  1. Viruses Infecting Reptiles

    OpenAIRE

    Marschang, Rachel E.

    2011-01-01

    A large number of viruses have been described in many different reptiles. These viruses include arboviruses that primarily infect mammals or birds as well as viruses that are specific for reptiles. Interest in arboviruses infecting reptiles has mainly focused on the role reptiles may play in the epidemiology of these viruses, especially over winter. Interest in reptile specific viruses has concentrated on both their importance for reptile medicine as well as virus taxonomy and evolution. The ...

  2. Oncogenic viruses and cancer

    Institute of Scientific and Technical Information of China (English)

    Guangxiang; George; Luo; Jing-hsiung; James; Ou

    2015-01-01

    <正>This special issue of the journal is dedicated to the important topic of oncogenic viruses and cancer.It contains seven review articles covering all known oncogenic viruses except for human T-lymphotropic virus type1(HTLV-1).These review articles are contributed by experts on specific viruses and their associated human cancers.Viruses account for about 20%of total human cancer cases.Although many viruses can cause various tumors in animals,only seven of them

  3. Virus Movement Maintains Local Virus Population Diversity

    Energy Technology Data Exchange (ETDEWEB)

    J. Snyder; B. Wiedenheft; M. Lavin; F. Roberto; J. Spuhler; A. Ortmann; T. Douglas; M. Young

    2007-11-01

    Viruses are the largest reservoir of genetic material on the planet, yet little is known about the population dynamics of any virus within its natural environment. Over a 2-year period, we monitored the diversity of two archaeal viruses found in hot springs within Yellowstone National Park (YNP). Both temporal phylogeny and neutral biodiversity models reveal that virus diversity in these local environments is not being maintained by mutation but rather by high rates of immigration from a globally distributed metacommunity. These results indicate that geographically isolated hot springs are readily able to exchange viruses. The importance of virus movement is supported by the detection of virus particles in air samples collected over YNP hot springs and by their detection in metacommunity sequencing projects conducted in the Sargasso Sea. Rapid rates of virus movement are not expected to be unique to these archaeal viruses but rather a common feature among virus metacommunities. The finding that virus immigration rather than mutation can dominate community structure has significant implications for understanding virus circulation and the role that viruses play in ecology and evolution by providing a reservoir of mobile genetic material.

  4. AL Amyloidosis

    Directory of Open Access Journals (Sweden)

    Desport Estelle

    2012-08-01

    Full Text Available Abstract Definition of the disease AL amyloidosis results from extra-cellular deposition of fibril-forming monoclonal immunoglobulin (Ig light chains (LC (most commonly of lambda isotype usually secreted by a small plasma cell clone. Most patients have evidence of isolated monoclonal gammopathy or smoldering myeloma, and the occurrence of AL amyloidosis in patients with symptomatic multiple myeloma or other B-cell lymphoproliferative disorders is unusual. The key event in the development of AL amyloidosis is the change in the secondary or tertiary structure of an abnormal monoclonal LC, which results in instable conformation. This conformational change is responsible for abnormal folding of the LC, rich in β leaves, which assemble into monomers that stack together to form amyloid fibrils. Epidemiology AL amyloidosis is the most common type of systemic amyloidois in developed countries with an estimated incidence of 9 cases/million inhabitant/year. The average age of diagnosed patients is 65 years and less than 10% of patients are under 50. Clinical description The clinical presentation is protean, because of the wide number of tissues or organs that may be affected. The most common presenting symptoms are asthenia and dyspnoea, which are poorly specific and may account for delayed diagnosis. Renal manifestations are the most frequent, affecting two thirds of patients at presentation. They are characterized by heavy proteinuria, with nephrotic syndrome and impaired renal function in half of the patients. Heart involvement, which is present at diagnosis in more than 50% of patients, leading to restrictive cardiopathy, is the most serious complication and engages prognosis. Diagnostic methods The diagnosis relies on pathological examination of an involved site showing Congo red-positive amyloid deposits, with typical apple-green birefringence under polarized light, that stain positive with an anti-LC antibody by immunohistochemistry and

  5. Risk behavior for the Human Immunodeficiency Virus among motel clients Las conductas de riesgo al virus de inmunodeficiencia humana entre usuarios de moteles Comportamentos de risco frente ao vírus da imunodeficiência humana entre frequentadores de motéis

    OpenAIRE

    Helisamara Mota Guedes; Luciana Oliveira Costa Cabral; Maria Verônica de Barros Costa; Alex Ferreira dos Reis; Simone Gomes Pereira; Fernanda Oliveira-Ferreira

    2012-01-01

    This study aimed to verify risk behavior for infection with the Human Immunodeficiency Virus in people who attended motels. This is a cross-sectional study conducted in two motels in two municipalities in the state of Minas Gerais, Brazil, with a sample of 308 randomly selected individuals, aged between 18 and 60 years. Bivariate (chi-square and t test for independent samples) and multivariate (logistic regression) statistical tests were performed. A total of 45.8% of the participants reporte...

  6. Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus

    OpenAIRE

    Jorge Madriz; José Bonilla; de Miranda, Joachim R.; Ana Espinoza

    2016-01-01

    Con el objetivo de determinar si el RHBV (Rice Hoja Blanca Virus) y el EHBV (Echinochloa Hoja Blanca Virus) se hallan relacionados serológicamente, se produjo un antisuero contra la proteína de nucleocápside del EHBV. Se demostró que ambos virus presentan características que los diferencian a este nivel. Al comparar la proteína de nucleocápside en geles SDS-PAGE de alta resolución, se observó que dicha proteína en el EHBV presenta una masa molecular menor que la del RHBV. El antisuero contra ...

  7. Virus como inductores de neoplasias cutáneas Viruses as agents inducing cutaneous neoplasms

    OpenAIRE

    Francisco Bravo Puccio

    2013-01-01

    El rol oncogénico de los virus en las neoplasias cutáneas es conocido por el hombre desde hace más de un siglo, cuando se atribuía el origen de la verruga vulgar al virus papiloma humano (VPH). En la actualidad, las neoplasias inducidas por virus pueden agruparse en tumores sólidos y procesos linfoproliferativos. Destacan entre los primeros el VPH, del cual ahora conocemos numerosos serotipos, cada uno vinculado a una neoplasia específica, el herpesvirus humano tipo 8 que produce el sarcoma d...

  8. Controversies about occult hepatitis B virus infection

    Institute of Scientific and Technical Information of China (English)

    Ersan Ozaslan; Tugrul Purnak

    2009-01-01

    We read with great interest the paper written by Shi et al, reviewing the molecular characteristics and stages of chronic hepatitis B virus (HBV) infection. We think that some points in the definition of occult HBV infection (OBI) and their conclusion about the management of OBI may need further considerations.

  9. Contribución de la anemia y de la exposición al virus de la inmunodeficiencia humana a la morbi-mortalidad infantil en África

    OpenAIRE

    Moraleda Redecilla, Cinta

    2015-01-01

    INTRODUCCIÓN Cada año mueren en el mundo más de 6 millones de niños menores de 5 años. Cerca del 50% en África subsahariana. Mejorar el conocimiento de patologías como la anemia y la exposición perinatal al VIH, que tienen un peso relevante pero poco reconocido en la mortalidad infantil, podría ayudar a reducir estas muertes. MATERIAL Y MÉTODOS Para determinar la etiología de anemia en Mozambique, profundizar en la etiopatogenia de la anemia asociada a malaria y determinar ...

  10. Síndrome linfoproliferativo ligado al cromosoma X, infección por el virus EBV y defectos en la regulación de la citotoxicidad linfocitaria X-linked lymphoproliferative syndrome, EBV infection and impaired regulation of cell-mediated cytotoxicity

    Directory of Open Access Journals (Sweden)

    A. Malbrán

    2003-01-01

    Full Text Available La deficiencia del gen SH2D1A que codifica para la proteína reguladora SAP trae aparejada la activación incontrolada de la vía de activación linfocitaria señalizada por SLAM (molécula señaladora de la activación linfocitaria. Es una inmunodeficiencia ligada al cromosoma X (XLP que se pone en evidencia cuando los pacientes portadores de mutaciones en el gen se enfrentan con el virus de Epstein Barr, desarrollando una mononucleosis infecciosa fulminante. Algunos pacientes desarrollan un síndrome linfoproliferativo fatal; los que sobreviven pueden presentar hipogammaglobulinemia severa y mayor frecuencia de neoplasia hematológica que la población normal. En esta revisión se discuten los mecanismos inmuno-regulatorios involucrados en el desarrollo de la patología mencionada, así como la participación de diferentes células efectoras de la respuesta inmune (linfocitos CD8 citotóxicos, células NK.Mutations in SH2D1A, a gene that codifies for the regulatory protein SAP, result in uncontrolled activation of the SLAM (signaling lymphocyte-activation molecule pathway. This X-linked immunodeficiency becomes evident when the patients are infected with Epstein Barr virus (EBV and develop a fulminant form of infectious mononucleosis leading to a lymphoproliferative syndrome that is often fatal (X-linked lymphoproliferative syndrome, XLP. In those who survive, hypogammaglobulinemia and oncohematologic diseases are frequently observed. In this revision, the immuno-regulatory mechanisms involved in XLP immunopathology and the role of different effector cells (CD8 T lymphocytes, NK cells are discussed.

  11. Restriction of Zika Virus by Host Innate Immunity.

    Science.gov (United States)

    Xie, Xuping; Shan, Chao; Shi, Pei-Yong

    2016-05-11

    Recent epidemics of Zika virus (ZIKV) have brought increasing concerns of heightened disease severity and neurotropism. In this issue of Cell Host & Microbe, Lazear et al. (2016) and Bayer et al. (2016) show that innate immunity can restrict ZIKV infection and disease development. PMID:27173920

  12. Viruses Infecting Reptiles

    Directory of Open Access Journals (Sweden)

    Rachel E. Marschang

    2011-11-01

    Full Text Available A large number of viruses have been described in many different reptiles. These viruses include arboviruses that primarily infect mammals or birds as well as viruses that are specific for reptiles. Interest in arboviruses infecting reptiles has mainly focused on the role reptiles may play in the epidemiology of these viruses, especially over winter. Interest in reptile specific viruses has concentrated on both their importance for reptile medicine as well as virus taxonomy and evolution. The impact of many viral infections on reptile health is not known. Koch’s postulates have only been fulfilled for a limited number of reptilian viruses. As diagnostic testing becomes more sensitive, multiple infections with various viruses and other infectious agents are also being detected. In most cases the interactions between these different agents are not known. This review provides an update on viruses described in reptiles, the animal species in which they have been detected, and what is known about their taxonomic positions.

  13. Compreendendo o ser familiar de criança com Vírus da Imunodeficiência Humana/ Síndrome da Imunodeficiência Adquirida pelo olhar da complexidade Comprendiendo al familiar de un niño afectado por el Virus de la Inmunodeficiencia Humana/Síndrome de la Inmunodeficiencia Adquirida, bajo la perspectiva de la complejidad Understanding the family member of a child affected by Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome, from the perspective of complexity

    Directory of Open Access Journals (Sweden)

    Hilda Maria Barbosa de Freitas

    2010-10-01

    Full Text Available OBJETIVO: Compreender o significado do ser familiar de uma criança com Vírus da Imunodeficiência Humana/ Síndrome da Imunodeficiência Adquirida na perspectiva do pensamento complexo. MÉTODOS: Estudo qualitativo, exploratório; os dados foram coletados, entre julho e novembro de 2008, por meio de uma entrevista com cinco familiares de crianças com AIDS, abrigadas em uma instituição de apoio. RESULTADOS: O significado das falas codificadas e analisadas resultou em três categorias: Vivenciando a ordem, desordem e organização do ser familiar de uma criança com AIDS; Dialogando com as certezas e incertezas do revelar ou não o diagnóstico à criança com AIDS; e Confrontando-se com os limites do acaso, ocaso, viver e morrer. CONCLUSÃO: Evidenciou-se que ser familiar e cuidar de uma criança com AIDS, constitui-se em um processo complexo que requer, além de uma compreensão biopsicossócio-espiritual dos fatores que envolvem a doença e o ser doente, uma atitude de cuidado focada no ser humano como um ser uno, complexo e multidimensional.OBJETIVO: Comprender el significado del familiar de un niño portador del Virus de la Inmunodeficiencia Humana/Síndrome de la Inmunodeficiencia Adquirida bajo la perspectiva del pensamiento complejo. MÉTODOS: Se trata de estudio cualitativo y exploratorio; los datos fueron recolectados entre julio y noviembre de 2008, por medio de entrevistas con cinco familiares de niños con SIDA, que estaban acogidos en una institución de apoyo. RESULTADOS: El significado de las declaraciones codificadas y analizadas resultó en tres categorías: experimentando el orden, desorden y la organización del familiar de un niño con SIDA; dialogando con la seguridad y la duda de revelar el diagnóstico al niño con SIDA; y, enfrentando los límites del acaso, ocaso, vivir y morir. CONCLUSIÓN: Se evidenció que ser familiar y cuidar de un niño con SIDA, constituye un proceso complejo que requiere, además de una

  14. Testing for Human Immunodeficiency Virus

    Science.gov (United States)

    ... education Fact Sheet PFS005: Testing for Human Immunodeficiency Virus AUGUST 2015 • Reasons for Getting Tested • Who Should ... For More Information • Glossary Testing for Human Immunodeficiency Virus Human immunodeficiency virus (HIV) is the virus that ...

  15. Analysis of Virus Algorithms

    Directory of Open Access Journals (Sweden)

    Jyoti Kalyani

    2006-01-01

    Full Text Available Security of wired and wireless networks is the most challengeable in today's computer world. The aim of this study was to give brief introduction about viruses and worms, their creators and characteristics of algorithms used by viruses. Here wired and wireless network viruses are elaborated. Also viruses are compared with human immune system. On the basis of this comparison four guidelines are given to detect viruses so that more secure systems are made. While concluding this study it is found that the security is most challengeable, thus it is required to make more secure models which automatically detect viruses and prevent the system from its affect.

  16. Hepatitis Delta Virus: A Peculiar Virus

    OpenAIRE

    Carolina Alves; Cristina Branco; Celso Cunha

    2013-01-01

    The hepatitis delta virus (HDV) is distributed worldwide and related to the most severe form of viral hepatitis. HDV is a satellite RNA virus dependent on hepatitis B surface antigens to assemble its envelope and thus form new virions and propagate infection. HDV has a small 1.7 Kb genome making it the smallest known human virus. This deceivingly simple virus has unique biological features and many aspects of its life cycle remain elusive. The present review endeavors to gather the available ...

  17. Expresión heteróloga de la proteína mayor de la cápsida (L1) del virus del papiloma humano tipo 18: purificación y caracterización de las proteínas recombinantes y partículas similares al virus (VLPS)

    OpenAIRE

    Martínez Martínez, Mónica

    2008-01-01

    La infección con el virus del papiloma humano (VPH) es la ETS más común en mujeres. Un tercio de los VPH mucosotrópicos, denominados de alto riesgo, están relacionados con el cáncer de cérvix, siendo el tipo 18 el segundo más. El 60% de las mujeres infectadas sufrirán seroconversión con anticuerpos neutralizantes dirigidos a epítopos conformacionales de la proteína mayoritaria de la cápsida (L1). Si bien los títulos de anticuerpos tras la infección natural son bajos, los adquiridos tras la va...

  18. Epstein-Barr virus infection mechanisms

    Institute of Scientific and Technical Information of China (English)

    Liudmila S. Chesnokova; Lindsey M. Hutt-Fletcher

    2014-01-01

    Epstein-Barr virus (EBV) infection occurs by distinct mechanisms across different cell types. EBV infection of B cellsin vitro minimally requires 5 viral glycoproteins and 2 cellular proteins. By contrast, infection of epithelial cells requires a minimum of 3 viral glycoproteins, which are capable of interacting with one or more of 3 different celular proteins. The ful complement of proteins involved in entry into al cel types capable of being infectedin vivo is unknown. This review discusses the events that occur when the virus is delivered into the cytoplasm of a cel, the players known to be involved in these events, and the ways in which these players are thought to function.

  19. Virus Infection

    Directory of Open Access Journals (Sweden)

    Hiroshi Abe

    2013-01-01

    Full Text Available Of 168 patients with chronic hepatitis B virus (HBV infection-related liver disease, 20 patients who had received 100 mg of lamivudine plus 10 mg/day of adefovir dipivoxil (ADV (ADV group and 124 patients who had received 0.5 mg/day of entecavir or 100 mg/day of lamivudine (non-ADV group for >1 year were enrolled. For comparative analyses, 19 well-matched pairs were obtained from the groups by propensity scores. At the time of enrollment, serum creatinine and phosphate concentrations were similar between the ADV and non-ADV groups; however, urinary phosphate ( and serum bone-specific alkaline phosphatase (BAP ( concentrations were significantly higher in the ADV group than in the non-ADV group. Serum BAP was significantly higher at the time of enrollment than before ADV administration in the ADV group (, although there was no significant change in serum BAP concentration in the non-ADV group. There was a significant positive correlation between the period of ADV therapy and ΔBAP (, . Serum BAP concentration increased before increase in serum creatinine concentration and was useful for early detection of adverse events and for developing adequate measures for continuing ADV for chronic HBV infection-related liver disease.

  20. Oscillations in serum ferritin associated with antiviral therapy in chronic hepatitis C Oscilaciones de la ferritina sérica asociadas al tratamiento antiviral en la hepatitis crónica por virus C

    Directory of Open Access Journals (Sweden)

    J. M. Ladero

    2009-01-01

    Full Text Available Background: hyperferritinemia is often found in patients with chronic hepatitis C (CHC and is predictive of poorer response to antiviral therapy. Objective: to investigate changes in ferritinemia during and after antiviral therapy. Patients and methods: serum ferritin levels were measured in 262 CHC patients (163 males, mean age 48.5 years ± 10.1 before and during antiviral therapy, and six months post-treatment in all 154 patients whit undetectable serum HCV-RNA after therapy completion. Results: baseline serum ferritin was higher in patients with primary therapeutic failure than in those reaching sustained viral response (330 ± 291 ng/mL vs. 211 ± 192 ng/mL, p = 0.002. Serum ferritin transiently increased during therapy from baseline (257 ± 242 ng/mL vs. 875 ± 630 ng/mL, p Antecedentes: la hiperferritinemia es frecuente en los enfermos con hepatitis crónica C (HCC y reduce las probabilidades de respuesta al tratamiento antiviral. Objetivo: investigar las variaciones de la ferritina sérica durante y después del tratamiento y su relación con la respuesta al mismo. Pacientes y métodos: la ferritina sérica se ha medido en 262 enfermos con HCC (163 hombres, edad media 48,5 años ± 10,1 antes y durante el tratamiento antiviral, y a los 6 meses de finalizado en los 154 enfermos con viremia indetectable al final del tratamiento. Resultados: la ferritina sérica basal era más alta en enfermos con fracaso terapéutico primario que en los que consiguieron respuesta viral sostenida (RVS (330 ± 291 ng/ml vs. 211 ± 192 ng/ml, p = 0,002. La ferritina sérica aumentó transitoriamente durante el tratamiento (257 ± 242 ng/ml vs. 875 ± 630 ng/ml, p < 0,001. La ferritina sérica descendió a valores inferiores a los basales seis meses después de finalizado el tratamiento en los pacientes con RVS (117 ± 102 ng/ml vs. 211± 192 ng/ml, p < 0,001 y, en menor grado, en los que sufrieron recidiva viral (217 ± 174 ng/ml vs. 257 ± 221 ng/m, p = 0

  1. Hepatitis virus panel

    Science.gov (United States)

    The hepatitis virus panel is a series of blood tests used to detect current or past infection by hepatitis A , hepatitis ... samples for more than one kind of hepatitis virus at the same time. Antibody and antigen tests ...

  2. Human Parainfluenza Viruses

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Human Parainfluenza Viruses (HPIVs) Note: Javascript is disabled or ... CDC.gov . Recommend on Facebook Tweet Share Compartir Human parainfluenza viruses (HPIVs) commonly cause respiratory illnesses in ...

  3. Tumorigenic DNA viruses

    Energy Technology Data Exchange (ETDEWEB)

    Klein, G.

    1989-01-01

    The eighth volume of Advances in Viral Oncology focuses on the three major DNA virus groups with a postulated or proven tumorigenic potential: papillomaviruses, animal hepatitis viruses, and the Epstein-Bar virus. In the opening chapters, the contributors analyze the evidence that papillomaviruses and animal hepatitis viruses are involved in tumorigenesis and describe the mechanisms that trigger virus-host cell interactions. A detailed section on the Epstein-Barr virus (EBV) - comprising more than half the book - examines the transcription and mRNA processing patterns of the virus genome; the mechanisms by which EBV infects lymphoid and epithelial cells; the immunological aspects of the virus; the actions of EBV in hosts with Acquired Immune Deficiency Syndrome; and the involvement of EBV in the etiology of Burkitt's lymphoma.

  4. Viruses and Breast Cancer

    International Nuclear Information System (INIS)

    Viruses are the accepted cause of many important cancers including cancers of the cervix and anogenital area, the liver, some lymphomas, head and neck cancers and indirectly human immunodeficiency virus associated cancers. For over 50 years, there have been serious attempts to identify viruses which may have a role in breast cancer. Despite these efforts, the establishment of conclusive evidence for such a role has been elusive. However, the development of extremely sophisticated new experimental techniques has allowed the recent development of evidence that human papilloma virus, Epstein-Barr virus, mouse mammary tumor virus and bovine leukemia virus may each have a role in the causation of human breast cancers. This is potentially good news as effective vaccines are already available to prevent infections from carcinogenic strains of human papilloma virus, which causes cancer of the uterine cervix

  5. Viruses and human cancer

    Energy Technology Data Exchange (ETDEWEB)

    Gallo, R.C.; Haseltine, W.; Klein, G.; Zur Hausen, H.

    1987-01-01

    This book contains papers on the following topics: Immunology and Epidemiology, Biology and Pathogenesis, Models of Pathogenesis and Treatment, Simian and Bovine Retroviruses, Human Papilloma Viruses, EBV and Herpesvirus, and Hepatitis B Virus.

  6. Herpes Simplex Virus (HSV)

    Science.gov (United States)

    ... rashes clinical tools newsletter | contact Share | Herpes Simplex Virus (HSV) A parent's guide to condition and treatment ... skin or mouth sores with the herpes simplex virus (HSV) is called primary herpes. This may be ...

  7. Hepatitis virus panel

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003558.htm Hepatitis virus panel To use the sharing features on this page, please enable JavaScript. The hepatitis virus panel is a series of blood tests used ...

  8. Viruses and Breast Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Lawson, James S., E-mail: james.lawson@unsw.edu.au; Heng, Benjamin [School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney (Australia)

    2010-04-30

    Viruses are the accepted cause of many important cancers including cancers of the cervix and anogenital area, the liver, some lymphomas, head and neck cancers and indirectly human immunodeficiency virus associated cancers. For over 50 years, there have been serious attempts to identify viruses which may have a role in breast cancer. Despite these efforts, the establishment of conclusive evidence for such a role has been elusive. However, the development of extremely sophisticated new experimental techniques has allowed the recent development of evidence that human papilloma virus, Epstein-Barr virus, mouse mammary tumor virus and bovine leukemia virus may each have a role in the causation of human breast cancers. This is potentially good news as effective vaccines are already available to prevent infections from carcinogenic strains of human papilloma virus, which causes cancer of the uterine cervix.

  9. Pseudacteon decapitating flies: Potential vectors of a fire ant virus?

    International Nuclear Information System (INIS)

    Solenopsis invicta virus (SINV-1) is a positive-stranded RNA virus recently found to infect all stages of the red imported fire ant, Solenopsis invicta (Valles et al. 2004; Valles and Strong 2005). SINV-1 and a second genotype have been tentatively assigned to the Dicistroviridae (Mayo 2002). Infected individuals or colonies did not exhibit any immediate, discernible symptoms in the field. However, under stress from introduction into the laboratory, brood death was often observed among infected colonies, ultimately leading to the death of the entire colony (Valles et al. 2004). These characteristics are consistent with other insect-infecting positive-stranded RNA viruses. They often persist as inapparent, asymptomatic infections that, under certain conditions, induce replication within the host, resulting in observable symptoms and often death (Christian and Scotti 1998; Fernandez et al. 2002). The SINV infection rate among colonies was reported to be around 25% in Gainesville, Florida (Valles et al. 2004; Valles and Strong 2005). SINV vertical and horizontal transmission were inferred based on RT-PCR detection of virus genome in eggs and successful colony to colony transfer under lab conditions (Valles et al. 2004). However, the exact mechanisms by which the virus is spread from nest to nest in the field are unknown. Our results indicate that SINV does not replicate within Pseudacteon decapitating flies that parasitize S. invicta. Flies appeared to develop normally from SINV-infected S. invicta workers. Mechanical transmission of SINV to uninfected ants by oviposition appears unlikely

  10. Complete nucleotide sequence of the new potexvirus "Alstroemeria virus X". Brief report.

    Science.gov (United States)

    Fuji, S; Shinoda, K; Ikeda, M; Furuya, H; Naito, H; Fukumoto, F

    2005-11-01

    A flexuous virus was isolated in Japan from an alstroemeria plant showing mosaic symptoms. The virus had a broad host range but had systemically latent infectivity in alstroemeria. The virus was assigned to the genus Potexvirus based on morphology and physical properties and on an analysis of the complete nucleotide sequence. The genomic RNA of the virus was 7,009 nucleotides in length, excluding the 3'-terminal poly (A) tail. It contained five open reading frames (ORFs), which was consistent with other members of the genus Potexvirus. Although nucleotide sequences of the ORFs differ from previously reported potexviruses, a phylogenetic analysis placed it phylogenetically close to Narcissus mosaic virus and Scallion virus X. Therefore, we propose that this virus should be designated as Alstroemeria virus X (AlsVX). PMID:15986173

  11. Evolución de la prevalencia de infección por virus de la inmunodeficiencia humana en población reclusa al ingreso en prisión durante el período 1991-1995

    Directory of Open Access Journals (Sweden)

    Martín Vicente

    1997-01-01

    Full Text Available FUNDAMENTO: Las prisiones españolas albergan un elevado número de usuarios de drogas por vía parenteral (UDVP, y otras personas con prácticas de riesgo para la infección por virus de la inmunodeficiencia humana (VIH. El objetivo de este trabajo es conocer la evolución de la prevalencia de infección por VIH en el momento del ingreso en prisión y los factores asociados a la misma en este colectivo, lo que puede permitir una aproximación a la efectividad de las estrategias de reducción de riesgos y contribuir a mejorarlas. MÉTODOS: Todas aquellas personas que ingresaron en un centro penitenciario provincial del noroeste español entre los años 1991 y 1995. Se recogieron variables socio-demográficas, penitenciarias y factores de riesgo para VIH. Previo consentimiento, se practicó prueba de infección por VIH (ELISA y Western-blot. RESULTADOS: El 19,4% de los 1663 individuos estudiados estaban infectados por VIH. Destacaban, con significación estadística, las prevalencias en: mujeres (26,0%, el grupo de edad desde 25 a 34 años (29,1%, blancos (20,9%, solteros (22,8%, tatuados (29,9%, los que presentaban antecedentes de autolesiones (42,4%, los UDVP (46,3%, los que reconocieron compartir jeringuillas (61,5 % y aquellos con antecedentes de haber permanecido en prisión un año o más (37,3%. El análisis de regresión logística mostró como predictores de infección VIH: los UDVP, los que ingresaron en 1992, las mujeres, los grupos de edad de 25-34 y de 35-44 años, los tatuados, los que presentaban antecedentes de autolesiones y aquellos con antecedentes de estancia en prisión superior a un año. La etnia gitana presentó menor probabilidad de infección por VIH. La tendencia temporal de la infección VIH estratificada según el antecedente de estancia previa en prisión, mostró un descenso casi significativo (P=0,064. La tendencia de la infección según UDVP no mostró modificación (P=0,16. CONCLUSIONES: Se ha detectado una

  12. Taming influenza viruses

    OpenAIRE

    Ozawa, Makoto; Kawaoka, Yoshihiro

    2011-01-01

    Plasmid-based reverse genetics systems allow the artificial generation of viruses with cloned cDNA-derived genomes. Since the establishment of such systems for influenza virus, numerous attempts have been made to tame this pathogenic agent. In particular, several types of viruses expressing foreign genes have been generated and used to further our knowledge of influenza virus replication and pathogenicity and to develop novel influenza vaccines. Here, we review these achievements and discuss ...

  13. Understanding Ebola Virus Transmission

    OpenAIRE

    Seth Judson; Joseph Prescott; Vincent Munster

    2015-01-01

    An unprecedented number of Ebola virus infections among healthcare workers and patients have raised questions about our understanding of Ebola virus transmission. Here, we explore different routes of Ebola virus transmission between people, summarizing the known epidemiological and experimental data. From this data, we expose important gaps in Ebola virus research pertinent to outbreak situations. We further propose experiments and methods of data collection that will enable scientists to fil...

  14. Hepatitis D Virus Replication.

    Science.gov (United States)

    Taylor, John M

    2015-11-01

    This work reviews specific related aspects of hepatitis delta virus (HDV) reproduction, including virion structure, the RNA genome, the mode of genome replication, the delta antigens, and the assembly of HDV using the envelope proteins of its helper virus, hepatitis B virus (HBV). These topics are considered with perspectives ranging from a history of discovery through to still-unsolved problems. HDV evolution, virus entry, and associated pathogenic potential and treatment of infections are considered in other articles in this collection. PMID:26525452

  15. Lifestyles of plant viruses

    OpenAIRE

    Roossinck, Marilyn J.

    2010-01-01

    The vast majority of well-characterized eukaryotic viruses are those that cause acute or chronic infections in humans and domestic plants and animals. However, asymptomatic persistent viruses have been described in animals, and are thought to be sources for emerging acute viruses. Although not previously described in these terms, there are also many viruses of plants that maintain a persistent lifestyle. They have been largely ignored because they do not generally cause disease. The persisten...

  16. Al(OH)_3和CpG ODN免疫佐剂对丙型肝炎病毒多表位抗原PCX3疫苗免疫效果的影响%The Immune Evaluation of Vaccine Adjuvants: Al(OH)_3 and CpG ODN on Multiple-Epitope Antigen of Hepatitis C Virus(PCX3)

    Institute of Scientific and Technical Information of China (English)

    祁淑红; 杨桂梅; 谢匡成; 刘龙丁; 任大明

    2009-01-01

    以HCV蛋白疫苗PCX3为抗原,分别加入Al(OH)_3佐剂,CpG ODN佐剂,以及两种佐剂联合作用的复合佐剂,对雄性六周龄BALB/c小鼠进行常规免疫.与其他免疫组相比,Al(OH)_3和Cp6 ODN复合佐剂与PCX3抗原联合作用组产生的特异性IgG(1×10~6)和IgM(1×10~5)抗体滴度最高、持续时间最长;自然杀伤效应(CTL)实验显示该组小鼠诱发的CTL杀伤效应(40.91%)显著高于其他免疫组(P<0.05);流式细胞仪对小鼠淋巴细胞亚群进行分型分析发现:从第2周开始,该组小鼠体内CD4~+/CD8~+细胞比值(3.26)显著高于PCX3抗原单独作用组(P<0.05).结果表明,Al(OH)_3和CpG ODN复合佐剂可以从体液免疫和细胞免疫两方面对HCV蛋白疫苗PCX3的免疫效果进行增强,并且具有协同效应.

  17. Chikungunya virus, Cameroon, 2006

    OpenAIRE

    Peyrefitte, Christophe N.; Rousset, Dominique; Pastorino, Boris A.M.; Pouillot, Regis; Bessaud, Maël; Tock, Fabienne; Mansaray, Helene; Merle, Olivier L.; Pascual, Aurelie M.; Paupy, Christophe; Vessiere, Aurelia; Imbert, Patrice; Tchendjou, Patrice; Durand, Jean-Paul; Tolou, Hugues J.

    2007-01-01

    We report the isolation of chikungunya virus from a patient during an outbreak of a denguelike syndrome in Cameroon in 2006. The virus was phylogenetically grouped in the Democratic Republic of the Congo cluster, indicating a continuous circulation of a genetically similar chikungunya virus population during 6 years in Central Africa.

  18. Computer Virus Protection

    Science.gov (United States)

    Rajala, Judith B.

    2004-01-01

    A computer virus is a program--a piece of executable code--that has the unique ability to replicate. Like biological viruses, computer viruses can spread quickly and are often difficult to eradicate. They can attach themselves to just about any type of file, and are spread by replicating and being sent from one individual to another. Simply having…

  19. What's West Nile Virus?

    Science.gov (United States)

    ... Help White House Lunch Recipes What's West Nile Virus? KidsHealth > For Kids > What's West Nile Virus? Print A A A Text Size en español ¿Qué es el Virus del Nilo Occidental? What exactly is the West ...

  20. Respiratory Syncytial Virus

    Science.gov (United States)

    ... Things to Know About Zika & Pregnancy Respiratory Syncytial Virus KidsHealth > For Parents > Respiratory Syncytial Virus Print A A A Text Size What's in ... RSV When to Call the Doctor en español Virus respiratorio sincitial About RSV Respiratory syncytial (sin-SISH- ...

  1. BOVINE VIRAL DIARRHEA VIRUSES

    Science.gov (United States)

    Bovine viral diarrhea virus (BVDV) is an umbrella term for two species of viruses, BVDV1 and BVDV2, within the Pestivirus genus of the Flavivirus family. BVDV viruses are further subclassified as cytopathic and noncytopathic based on their activity in cultured epithelial cells. Noncytopathic BVDV p...

  2. Infección experimental con el virus de la diarrea viral bovina (VDVB genotipo 2 en terneros con anticuerpos neutralizantes al VDVB genotipo 1 Experimental infection with bovine viral diarrhea virus (BVDV genotype 2 in calves with neutralizing antibodies to BVDV genotype 1

    Directory of Open Access Journals (Sweden)

    J.I. Ronchi

    2001-01-01

    Full Text Available The aim of this study was to determine the consequences of the infection of BVDV genotype II (BVDV-2 in calves with preexisting immunity to BVDV-1 (induced by infection with wild type BVDV (Group A, 4 calves, or induced by vaccination with a killed BVDV-1 product (Group B, 2 calves. Group A and B were intranasally exposed to a field isolate of BVDV-2 (25ml x 10-7.8 TCID50/ml. Calves of Group A had erosions on the oral mucosa from 3 to 4 day post-challenge (DPC; moreover, intense salivation, mucous nasal secretion and sporadic diarrhea were observed from 4-14 DPC. Only one calf of Group B had diarrhea at 5 DPC. The average value of peripheral blood leukocytes (PBL previous to BVDV-2 challenge was 8,775 PBL/ml (±603 in calves of Group A and 9,975 PBL/ml (±1308 in calves of Group B. After BVDV-2 challenge there was leukopenia in Groups A and B. The minimal number of PBL of 5,875 PBL/ml (±311 was observed in Group A at 6 PDC (P <0.05. There were differences (P <0,05 in the average of PBL during the 17 DPC among challenged animals and uninfected controls. Moreover, a difference in the number of blood lymphocytes was observed at 6-7 DPC between virus exposed and control calves. BVDV was isolated from ocular swabs of 3 calves of Group A at 13 and 17 DPC and from PBL at 9 and 11 DPC. The neutralizing antibody titer (NT to both genotypes of BVDV was higher (P <0.05 in calves of Group A. Previous to virus challenge calves in this group had NT of 1:125 (geometric mean and 1:128 to BVDV-1 and -II, respectively. The NT rose to 1:2048 at 17 DPC to both Genotypes of BVDV. Calves in Group B had a NT of 1:16 to both BVDV-1 and -II previous to BVDV-2 challenge. At 17 DPC the NT rose to 1:64 and to 1:181 to BVDV-1 and -II, respectively. These results allow us to conclude that antibodies generated by natural infection or vaccination with BVDV-1 increased for BVDV-1 and -2 after the experimental challenge with BVDV-2.

  3. Utilidade da teoria de autocuidado na assistência ao portador do Vírus da Imunodeficiência Humana/ Síndrome da Imunodeficiência Adquirida Utilidad de la teoría del autocuidado en la asistencia al portador del Virus de la Inmunodeficiencia Humana/Síndrome de la Inmunodeficiencia Adquirida Utility of the self-care theory to assist the bearer of Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome

    Directory of Open Access Journals (Sweden)

    Léa Maria Moura Barroso

    2010-01-01

    Full Text Available OBJETIVO: Analisar dentro da Teoria geral de Orem, a utilidade da Teoria de Autocuidado para pacientes com Vírus da Imunodeficiência Humana/ Síndrome da Imunodeficiência Adquirida (HIV/AIDS, segundo o modelo de análise de teorias MÉTODOS: Estudo de revisão bibliográfica realizado nas Bases de Dados LILACS, MEDLINE e BDENF com as palavras-chave: autocuidado, Orem e HIV/AIDS. Adotou-se o modelo de análise da teoria de Meleis focando o componente utilidade. Foi identificado um estudo que utilizou a Teoria de Orem com pacientes portadores de HIV/AIDS. RESULTADOS: A teoria demonstrou ser útil para orientar a sistematização da assistência de enfermagem ao portador de HIV/AIDS; construir instrumentos de avaliação e orientação; medir a qualidade da assistência; testar modelos; classificar o paciente e ajudar como apoio-educação. CONCLUSÃO: O modelo de Meleis possibilitou compreender a utilidade da Teoria de Orem para a prática, pesquisa, educação e administração.OBJETIVO: Analizar dentro de la Teoría general de Orem, la utilidad de la Teoría del Auto-cuidado para pacientes con Virus de la Inmunodeficiencia Humana/Síndrome de la Inmunodeficiencia Adquirida (HIV/SIDA, según el modelo de análisis de teorías. MÉTODOS: Se trata de un estudio de revisión bibliográfica realizado en las Bases de Datos LILACS, MEDLINE y BDENF con las palabras clave: auto-cuidado, Orem y HIV/SIDA. Se adoptó el modelo de análisis de la teoría de Meleis enfocando el componente utilidad. Fue identificado un estudio que utilizó la Teoría de Orem con pacientes portadores de HIV/SIDA. RESULTADOS: La teoría demostró ser útil para: orientar la sistematización de la asistencia de enfermería al portador de HIV/SIDA; construir instrumentos de evaluación y orientación; medir la calidad de la asistencia; comprobar modelos; clasificar el paciente; y, ayudar como apoyo para la educación. CONCLUSIÓN: El modelo de Meleis posibilitó comprender la

  4. Understanding Ebola Virus Transmission

    Directory of Open Access Journals (Sweden)

    Seth Judson

    2015-02-01

    Full Text Available An unprecedented number of Ebola virus infections among healthcare workers and patients have raised questions about our understanding of Ebola virus transmission. Here, we explore different routes of Ebola virus transmission between people, summarizing the known epidemiological and experimental data. From this data, we expose important gaps in Ebola virus research pertinent to outbreak situations. We further propose experiments and methods of data collection that will enable scientists to fill these voids in our knowledge about the transmission of Ebola virus.

  5. Viruses of asparagus.

    Science.gov (United States)

    Tomassoli, Laura; Tiberini, Antonio; Vetten, Heinrich-Josef

    2012-01-01

    The current knowledge on viruses infecting asparagus (Asparagus officinalis) is reviewed. Over half a century, nine virus species belonging to the genera Ilarvirus, Cucumovirus, Nepovirus, Tobamovirus, Potexvirus, and Potyvirus have been found in this crop. The potyvirus Asparagus virus 1 (AV1) and the ilarvirus Asparagus virus 2 (AV2) are widespread and negatively affect the economic life of asparagus crops reducing yield and increasing the susceptibility to biotic and abiotic stress. The main properties and epidemiology of AV1 and AV2 as well as diagnostic techniques for their detection and identification are described. Minor viruses and control are briefly outlined. PMID:22682173

  6. Online identification of viruses.

    Science.gov (United States)

    Kolaskar, A S; Naik, P S

    2000-06-01

    A computerized animal virus information system is developed in the Sequence Retrieval System (SRS) format. This database is available on the Word Wide Web (WWW) at the site http://bioinfo.ernet.in/www/avis/avis++ +.html. The database has been used to generate large number of identification matrices for each family. The software is developed in C. Unix shell scripts and Hypertext Marked-up Language (HTML) to assign the family to an unknown virus deterministically and to identify the virus probabilistically. It has been shown that such web based virus identification approach provides results with high confidence in those cases where identification matrix uses large number of independent characters. Protein sequence data for animal viruses have been analyzed and oligopeptides specific to each virus family and also specific to each virus species are identified for several viruses. These peptides thus could be used to identify the virus and to assign the virus family with high confidence showing the usefulness of sequence data in virus identification. PMID:10917875

  7. Protoplasts and plant viruses

    International Nuclear Information System (INIS)

    The use of protoplasts in the study of plant viruses has attracted considerable attention since its inception in the late 1960s. This article is an attempt to assess the current status of protoplasts (primarily) and all cell cultures (in some instances) in studies of virus infection, virus replication, cytopathology, cross-protection, virus resistance, and the use of in vitro methods and genetic engineering to recover virus-resistant plants. These areas of study proved difficult to do entirely with whole plants or plant parts. However, because protoplasts could be synchronously infected with virus, they provided a valuable alternative means of following biochemical and cytological events in relation to the virus growth cycle in a more precise manner than previously possible

  8. All About ALS

    Science.gov (United States)

    ... please review our exit disclaimer . Subscribe All About ALS Understanding a Devastating Disorder In the summer of ... of research dollars—for a fatal disease called ALS. ALS stands for amyotrophic lateral sclerosis. It’s also ...

  9. Serological relationships between rice Hoja Blanca Virus AND Echinochloa Hoja Blanca Virus

    Directory of Open Access Journals (Sweden)

    Jorge Madriz

    2016-03-01

    Full Text Available Con el objetivo de determinar si el RHBV (Rice Hoja Blanca Virus y el EHBV (Echinochloa Hoja Blanca Virus se hallan relacionados serológicamente, se produjo un antisuero contra la proteína de nucleocápside del EHBV. Se demostró que ambos virus presentan características que los diferencian a este nivel. Al comparar la proteína de nucleocápside en geles SDS-PAGE de alta resolución, se observó que dicha proteína en el EHBV presenta una masa molecular menor que la del RHBV. El antisuero contra la proteína N del RHBV producido por Espinoza et al. (1992 reconoce la del EHBV por Western blot. Lo anterior indica que son similares y sugiere la presencia de epitopos comunes; además confirma que el EHBV presenta una menor masa molecular que el RHBV. Asimismo, el método de inmunodifusión doble de OUCHTERLONY mostró una identidad parcial de las nucleoproteínas de ambos virus, lo que confirma que ambos virus se encuentran serológicamente relacionados.

  10. Detección molecular del virus de la hepatitis E en hígados de cerdo destinados al consumo humano en el estado de Nuevo León, México Molecular detection of hepatitis E virus in pig livers destined for human consumption in the state of Nuevo Leon, Mexico

    Directory of Open Access Journals (Sweden)

    Marco Antonio Cantú-Martínez

    2013-04-01

    Full Text Available OBJETIVO: Detección molecular del virus de la hepatitis E (VHE en hígado de cerdo para consumo humano en Nuevo León, México. MATERIAL Y MÉTODOS Se analizaron 127 hígados de cerdo (87 obtenidos de rastros TIF, y 40 de carnicerías mediante RT-PCR semianidado para amplificar un fragmento de 212 pb del gen ORF2 del VHE. RESULTADOS: El 19.5% (17 de los hígados de rastros y 22.5% (9 de carnicerías fueron positivos. La secuenciación mostró 94-95% de homología con el genotipo 3. CONCLUSIONES: Los resultados indican que el VHE circula en granjas porcinas del estado, lo que constituye una probable fuente de contaminación para los productos cárnicos porcinos.OBJECTIVE: Molecular detection of HEV in pig livers destined for human consumption in Nuevo Leon, Mexico. MATERIALS AND METHODS: 87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEV ORF2 gene was amplified by semi-nested RT-PCR. RESULTS: 19.54% (17 of tif's and 22.5% (9 of buthcer's livers were positive for HEV. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3. CONCLUSIONS: Our results indicate that HEV is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.

  11. Activation of human immunodeficiency virus by radiation

    International Nuclear Information System (INIS)

    It was recently demonstrated that ultraviolet radiation (UVR) can induce the HIV promoter as well as activate the complete virus in cultured cells (Valerie et al., 1988). This and subsequent observations, reviewed in this article, suggest a possibility that radiation exposure may accelerate development of AIDS in HIV-infected individuals. They also indicate that studies on HIV activation by stressors, including radiation, may advance our understanding of some phenomena that follow HIV infection. (author)

  12. RNA Viruses Infecting Pest Insects

    Science.gov (United States)

    RNA viruses are viruses whose genetic material is ribonucleic acid (RNA). RNA viruses may be double or single-stranded based on the type of RNA they contain. Single-stranded RNA viruses can be further grouped into negative sense or positive-sense viruses according to the polarity of their RNA. Fur...

  13. Characterization of foot-and-mouth disease virus types Ο and Asia 1 RNA

    OpenAIRE

    S. Vasantha; LAL, SM; Antony, A

    1988-01-01

    Foot-and-mouth disease is an acute and highly contagious febrile disease affecting cloven-footed animals. Identification of the foot-and-mouth disease virus (FMDV), the causative agent of the disease, posed problems because of the occurrence of many types and subtypes of the virus. A molecular approach based on oligonucleotide mapping of FMDV RNA has been used for the identification and characterization of virus isolates obtained in a disease outbreak (King et al., 1981). One-dimensiona...

  14. Mutational changes in the vesicular stomatitis virus glycoprotein affect the requirement of carbohydrate in morphogenesis.

    OpenAIRE

    Chatis, P A; Morrison, T G

    1981-01-01

    The role of carbohydrate in the morphogenesis of vesicular stomatitis virus was studied, using the antibiotic tunicamycin to inhibit glycosylation. It has been reported previously (Gibson et al., J. Biol. Chem. 254:3600-3607, 1979) that the San Juan strain of vesicular stomatitis virus requires carbohydrate for efficient migration of the glycoprotein (G) to the cell surface and for virion formation, whereas the prototype or Orsay strain of vesicular stomatitis virus is less stringent in its c...

  15. Face singular do cuidado familiar à criança portadora do vírus HIV/AIDS Cara singular del Cuidado Familiar al niño Portador del Vírus VIH/ SIDA Singular view of family care for children with the HIV / AIDS virus

    Directory of Open Access Journals (Sweden)

    Giovana Calcagno Gomes

    2012-01-01

    Full Text Available OBJETIVO: Conhecer as vivências da família no cuidado à criança portadora do vírus HIV/AIDS. MÉTODOS: Foi realizado em um hospital universitário no primeiro semestre de 2010. Participaram sete familiares cuidadores. Os dados foram coletados por entrevistas semiestruturadas e analisados pela técnica de Análise Temática. RESULTADOS: Evidenciou-se o silenciamento do diagnóstico pelo familiar cuidador que passa a viver em função do cuidado à criança. Como dificuldades para cuidar, destacam-se as condições de saúde da mãe ou sua morte, as hospitalizações como comprometedoras da escolarização e reveladoras do diagnóstico e o medo em contar o diagnóstico à criança. CONCLUSÃO: Acredita-se que os profissionais da saúde/enfermagem necessitam empregar estratégias que possibilitem a essas famílias melhor enfrentamento do cotidiano, assessorando-as para o cuidado, fornecendo informações sobre o HIV/AIDS às pessoas de seu círculo social, diminuindo o estigma e a discriminação a que estão expostas essas crianças.OBJETIVO: Conocer las vivencias de la familia en el cuidado del niño portador del virus VIH/SIDA. MÉTODOS: Fue realizado en un hospital universitario en el primer semestre de 2010. Participaron siete familiares cuidadores. Los datos fueron recolectados por medio de entrevistas semiestructuradas y analizadas por la técnica de Análisis Temático. RESULTADOS: Se evidenció el silenciamiento del diagnóstico por parte del familiar cuidador quien pasa a vivir en función del cuidado al niño. Como dificultades para cuidar, se destacan las condiciones de salud de la madre o su muerte, las hospitalizaciones como comprometedoras de la escolarización y reveladoras del diagnóstico y el miedo para comunicar el diagnóstico al niño. CONCLUSIÓN: Se cree que los profesionales de la salud/enfermería necesitan emplear estrategias que posibiliten a esas familias un mejor enfrentamiento del cotidiano, asesorándolas para

  16. Host Restriction Factor Screening – Let the Virus Do the Work

    OpenAIRE

    Diamond, Michael S.; Schoggins, John W.

    2013-01-01

    In this issue of Cell Host & Microbe, Varble et al 2013, engineer a library of RNA viruses to express small interfering RNAs and couple this with the power of virus evolution and selection to screen for host genes that when silenced resulted in greater viral infection in vivo.

  17. Constructing computer virus phylogenies

    Energy Technology Data Exchange (ETDEWEB)

    Goldberg, L.A. [Warwick Univ., Coventry (United Kingdom) Dept. of Computer Science; Goldberg, P.W. [Aston Univ., Birmingham (United Kingdom) Dept. of Applied Mathematics; Phillips, C.A. [Sandia National Labs., Albuquerque, NM (United States); Sorkin, G.B. [International Business Machines Corp., Yorktown Heights, NY (United States). Thomas J. Watson Research Center

    1996-03-01

    There has been much recent algorithmic work on the problem of reconstructing the evolutionary history of biological species. Computer virus specialists are interested in finding the evolutionary history of computer viruses--a virus is often written using code fragments from one or more other viruses, which are its immediate ancestors. A phylogeny for a collection of computer viruses is a directed acyclic graph whose nodes are the viruses and whose edges map ancestors to descendants and satisfy the property that each code fragment is ``invented`` only once. To provide a simple explanation for the data, we consider the problem of constructing such a phylogeny with a minimal number of edges. In general, this optimization problem cannot be solved in quasi-polynomial time unless NQP=QP; we present positive and negative results for associated approximated problems. When tree solutions exist, they can be constructed and randomly sampled in polynomial time.

  18. Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus, and human immunodeficiency virus.

    OpenAIRE

    Baba, M.; Snoeck, R; Pauwels, R; De Clercq, E

    1988-01-01

    Several sulfated polysaccharides (dextran sulfate, pentosan polysulfate, fucoidan, and carrageenans) proved to be potent inhibitors for herpes simplex virus, human cytomegalovirus, vesicular stomatitis virus, Sindbis virus, and human immunodeficiency virus. They were moderately inhibitory to vaccinia virus but not inhibitory to adenovirus, coxsackievirus, poliovirus, parainfluenza virus, and reovirus. These results indicate that, with the exception of parainfluenza virus, enveloped viruses ar...

  19. Assessing Seasonal Risks for the Introduction and Mosquito-borne Spread of Zika Virus in Europe

    Directory of Open Access Journals (Sweden)

    Joacim Rocklöv

    2016-07-01

    Full Text Available The explosive Zika virus epidemic in the Americas is amplifying spread of this emerging pathogen into previously unaffected regions of the world, including Europe (Gulland, 2016, where local populations are immunologically naïve. As summertime approaches in the northern hemisphere, Aedes mosquitoes in Europe may find suitable climatic conditions to acquire and subsequently transmit Zika virus from viremic travellers to local populations. While Aedes albopictus has proven to be a vector for the transmission of dengue and chikungunya viruses in Europe (Delisle et al., 2015; ECDC, n.d. there is growing experimental and ecological evidence to suggest that it may also be competent for Zika virus(Chouin-Carneiro et al., 2016; Grard et al., 2014; Li et al., 2012; Wong et al., 2013. Here we analyze and overlay the monthly flows of airline travellers arriving into European cities from Zika affected areas across the Americas, the predicted monthly estimates of the basic reproduction number of Zika virus in areas where Aedes mosquito populations reside in Europe (Aedes aegypti in Madeira, Portugal and Ae. albopictus in continental Europe, and human populations living within areas where mosquito-borne transmission of Zika virus may be possible. We highlight specific geographic areas and timing of risk for Zika virus introduction and possible spread within Europe to inform the efficient use of human disease surveillance, vector surveillance and control, and public education resources.

  20. Assessing Seasonal Risks for the Introduction and Mosquito-borne Spread of Zika Virus in Europe.

    Science.gov (United States)

    Rocklöv, Joacim; Quam, Mikkel Brandon; Sudre, Bertrand; German, Matthew; Kraemer, Moritz U G; Brady, Oliver; Bogoch, Isaac I; Liu-Helmersson, Jing; Wilder-Smith, Annelies; Semenza, Jan C; Ong, Mark; Aaslav, Kaja Kaasik; Khan, Kamran

    2016-07-01

    The explosive Zika virus epidemic in the Americas is amplifying spread of this emerging pathogen into previously unaffected regions of the world, including Europe (Gulland, 2016), where local populations are immunologically naïve. As summertime approaches in the northern hemisphere, Aedes mosquitoes in Europe may find suitable climatic conditions to acquire and subsequently transmit Zika virus from viremic travellers to local populations. While Aedes albopictus has proven to be a vector for the transmission of dengue and chikungunya viruses in Europe (Delisle et al., 2015; ECDC, n.d.) there is growing experimental and ecological evidence to suggest that it may also be competent for Zika virus(Chouin-Carneiro et al., 2016; Grard et al., 2014; Li et al., 2012; Wong et al., 2013). Here we analyze and overlay the monthly flows of airline travellers arriving into European cities from Zika affected areas across the Americas, the predicted monthly estimates of the basic reproduction number of Zika virus in areas where Aedes mosquito populations reside in Europe (Aedes aegypti in Madeira, Portugal and Ae. albopictus in continental Europe), and human populations living within areas where mosquito-borne transmission of Zika virus may be possible. We highlight specific geographic areas and timing of risk for Zika virus introduction and possible spread within Europe to inform the efficient use of human disease surveillance, vector surveillance and control, and public education resources. PMID:27344225

  1. Zika virus - an overview.

    Science.gov (United States)

    Zanluca, Camila; Dos Santos, Claudia Nunes Duarte

    2016-05-01

    Zika virus (ZIKV) is currently one of the most important emerging viruses in the world. Recently, it has caused outbreaks and epidemics, and has been associated with severe clinical manifestations and congenital malformations. However to date, little is known about the pathogenicity of the virus and the consequences of ZIKV infection. In this paper, we provide an overview of the current knowledge on ZIKV. PMID:26993028

  2. The Influenza Virus Enigma

    OpenAIRE

    Salomon, Rachelle; Webster, Robert G.

    2009-01-01

    Both seasonal and pandemic influenza continue to challenge both scientists and clinicians. Drug-resistant H1N1 influenza viruses have dominated the 2009 flu season, and the H5N1 avian influenza virus continues to kill both people and poultry in Eurasia. Here, we discuss the pathogenesis and transmissibility of influenza viruses and we emphasize the need to find better predictors of both seasonal and potentially pandemic influenza.

  3. Endocytosis of influenza viruses

    OpenAIRE

    Lakadamyali, Melike; Rust, Michael J.; Zhuang, Xiaowei

    2004-01-01

    Receptor-mediated endocytosis is known to play an important role in the entry of many viruses into host cells. However, the exact internalization mechanism has, until recently, remained poorly understood for many medically important viruses, including influenza. Developments in real-time imaging of single viruses as well as the use of dominant negative mutants to selectively block specific endocytic pathways, have improved our understanding of the influenza infection process.

  4. Filamentous influenza viruses

    OpenAIRE

    Dadonaite, Bernadeta; Vijyakrishnan, Swetha; Fodor, Ervin; Bhella, David; Hutchinson, Edward C.

    2016-01-01

    Clinical isolates of influenza virus produce pleomorphic virus particles, including extremely long filamentous virions. In contrast, strains of influenza that have adapted to laboratory growth typically produce only spherical virions. As a result, the filamentous phenotype has been overlooked in most influenza virus research. Recent advances in imaging and improved animal models have highlighted the distinct structure and functional relevance of filamentous virions. In this review we summaris...

  5. The virus of management

    OpenAIRE

    Kjær, Peter; Frankel, Christian

    2004-01-01

    The virus metaphor may be used in studies of management knowledge not only as a way of describing diffusion processes but also as a way of thinking about viral elements of knowledge production. In the present article, organizational viruses are viewed as ensembles of basic distinctions that are constitutive of concrete bodies of knowledge and which form mutable engines of organizational self-descriptions. Organizational viruses, we contend, are both characterized by stability i...

  6. Viruses in Turing's Garden

    OpenAIRE

    Marion, Jean-Yves

    2012-01-01

    Cohen and his supervisor Adleman defined a virus as follows: "A virus is a program that is able to infect other programs by modifying them to include a possibly evolved copy to itself". This definition seems to be well accepted by the computer security community as a foundational definition. Thus, a virus is a self-replicating program, whose offspring may be a mutation of the original program. Viruses thrive in our computers, which are based on Turing's model of computation. We discuss the fu...

  7. Residual viruses in pork products.

    Science.gov (United States)

    McKercher, P D; Hess, W R; Hamdy, F

    1978-01-01

    Partly cooked canned hams and dried pepperoni and salami sausages were prepared from the carcasses of pigs infected with African swine fever virus and pigs infected with hog cholera virus. Virus was not recovered from the partly cooked canned hams; however, virus was recovered in the hams before heating in both instances. Both African swine fever virus and hog cholera virus were recovered from the dried salami and pepperoni sausages, but not after the required curing period. PMID:564162

  8. FAQ: General Questions about West Nile Virus

    Science.gov (United States)

    ... Public Service Videos General Questions About West Nile Virus Recommend on Facebook Tweet Share Compartir On this ... West Nile virus cases? What is West Nile virus? West Nile virus is an arthropod-borne virus ( ...

  9. Viral Haemorrhagic Septicaemia Virus

    DEFF Research Database (Denmark)

    Olesen, Niels Jørgen; Skall, Helle Frank

    This chapter covers the genetics (genotypes and serotypes), clinical signs, host species, transmission, prevalence, diagnosis, control and prevention of viral haemorrhagic septicaemia virus.......This chapter covers the genetics (genotypes and serotypes), clinical signs, host species, transmission, prevalence, diagnosis, control and prevention of viral haemorrhagic septicaemia virus....

  10. INFLUENZA VIRUS IN POULTRY

    Science.gov (United States)

    Avian influenza virus (AIV) is normally found in wild birds, particularly in ducks and shorebirds, where it does not cause any perceptible clinical disease. However, poultry, including chickens and turkeys, are not normal hosts for avian influenza, but if the virus is introduced it can result in mi...

  11. Zika Virus and Pregnancy.

    Science.gov (United States)

    Stagg, Denise; Hurst, Helen M

    2016-01-01

    Recent outbreaks of Zika virus and reports linking infection in pregnant women with microcephaly in newborns have caused concern worldwide. Information has been evolving rapidly. Nurses and other clinicians, especially those who work with women of childbearing age, play a pivotal role in disseminating accurate information and identifying potential cases of Zika virus infection. PMID:27287356

  12. Papaya ringspot virus (Potyviridae)

    Science.gov (United States)

    Papaya ringspot virus, a member of the family Potyviridae, is single stranded RNA plant virus with a monocistronic genome of about 10,326 nucleotides that is expressed via a large polyprotein subsequently cleaved into functional proteins. It causes severe damage on cucurbit crops such as squash and...

  13. Viral Haemorrhagic Septicaemia Virus

    DEFF Research Database (Denmark)

    Olesen, Niels Jørgen; Skall, Helle Frank

    2013-01-01

    This chapter covers the genetics (genotypes and serotypes), clinical signs, host species, transmission, prevalence, diagnosis, control and prevention of viral haemorrhagic septicaemia virus.......This chapter covers the genetics (genotypes and serotypes), clinical signs, host species, transmission, prevalence, diagnosis, control and prevention of viral haemorrhagic septicaemia virus....

  14. Rift Valley Fever Virus

    Science.gov (United States)

    Rift Valley fever virus (RVFV) is a mosquito-transmitted virus or arbovirus that is endemic in sub-Saharan Africa. In the last decade, Rift Valley fever (RVF) outbreaks have resulted in loss of human and animal life, as well as had significant economic impact. The disease in livestock is primarily a...

  15. Aussprache als motorische Fertigkeit

    DEFF Research Database (Denmark)

    Bonner, Maria

    2013-01-01

    Authentische Aussprache gilt im Fremdsprachenunterricht als schwer erreichbares Ziel, fremdsprachlicher Akzent wird als nahezu unvermeidbar gesehen. Weder die Hypothese einer kritischen Periode für den Erwerb einer authentischen Aussprache noch das Konzept der Fossilierung im Sprachlernprozess...

  16. Amyotrophic Lateral Sclerosis (ALS)

    Science.gov (United States)

    ... benefit of 16 months before onset of severe respiratory failure. Other treatments are designed to relieve symptoms and ... of ALS. Most people with ALS die from respiratory failure, usually within 3 to 5 years from the ...

  17. Initial Symptoms of ALS

    Science.gov (United States)

    ... Chapters Certified Centers and Clinics Support Groups About ALS About Us Our Research In Your Community Advocate ... Diagnosis En español Symptoms The initial symptoms of ALS can be quite varied in different people. One ...

  18. Varroa destructor Macula-like virus, Lake Sinai virus and other new RNA viruses in wild bumblebee hosts (Bombus pascuorum, Bombus lapidarius and Bombus pratorum).

    Science.gov (United States)

    Parmentier, Laurian; Smagghe, Guy; de Graaf, Dirk C; Meeus, Ivan

    2016-02-01

    Pollinators such as bumblebees (Bombus spp.) are in decline worldwide which poses a threat not only for ecosystem biodiversity but also to human crop production services. One main cause of pollinator decline may be the infection and transmission of diseases including RNA viruses. Recently, new viruses have been discovered in honeybees, but information on the presence of these in wild bumblebees is largely not available. In this study, we investigated the prevalence of new RNA viruses in Bombus species, and can report for the first time Varroa destructor Macula-like virus (VdMLV) and Lake Sinai virus (LSV) infection in multiple wild bumblebee hosts of Bombus pascuorum, Bombus lapidarius and Bombus pratorum. We sampled in 4 locations in Flanders, Belgium. Besides, we confirmed Slow bee paralysis virus (SBPV) in wild bumblebees, but no positive samples were obtained for Big Sioux river virus (BSRV). Secondly, we screened for the influence of apiaries on the prevalence of these viruses. Our results indicated a location effect for the prevalence of VdMLV in Bombus species, with a higher prevalence in the proximity of honeybee apiaries mainly observed in one location. For LSV, the prevalence was not different in the proximity or at a 1.5 km-distance of apiaries, but we reported a different isolate with similarities to LSV-2 and "LSV-clade A" as described by Ravoet et al. (2015), which was detected both in Apis mellifera and Bombus species. In general, our results indicate the existence of a disease pool of new viruses that seems to be associated to a broad range of Apoidae hosts, including multiple Bombus species. PMID:26706994

  19. Nucleotide sequence of the coat protein genes of alstroemeria mosaic virus and amazon lily mosaic virus, a tentative species of genus potyvirus.

    Science.gov (United States)

    Fuji, S; Terami, F; Furuya, H; Naito, H; Fukumoto, F

    2004-09-01

    The nucleotide sequences of the 3' terminal region of the genomes of Alstroemeria mosaic virus (AlsMV) and the Amazon lily mosaic virus (ALiMV) have been determined. These sequences contain the complete coding region of the viral coat protein (CP) gene followed by a 3'-untranslated region (3'-UTR). AlsMV and ALiMV share 74.9% identity in the amino acid sequence of the CP, and 55.6% identity in the nucleotide sequence of the 3'-UTR. Phylogenetic analysis of these CP genes and 3'-UTRs in relation to those of 79 potyvirus species revealed that AlsMV and ALiMV should be assigned to the Potato virus Y (PVY) subgroup. AlsMV and ALiMV were concluded to have arisen independently within the PVY subgroup. PMID:15593424

  20. Computer Viruses: Pathology and Detection.

    Science.gov (United States)

    Maxwell, John R.; Lamon, William E.

    1992-01-01

    Explains how computer viruses were originally created, how a computer can become infected by a virus, how viruses operate, symptoms that indicate a computer is infected, how to detect and remove viruses, and how to prevent a reinfection. A sidebar lists eight antivirus resources. (four references) (LRW)

  1. Postmortem Stability of Ebola Virus

    OpenAIRE

    Prescott, Joseph; Bushmaker, Trenton; Fischer, Robert; Miazgowicz, Kerri; Judson, Seth; Vincent J. Munster

    2015-01-01

    The ongoing Ebola virus outbreak in West Africa has highlighted questions regarding stability of the virus and detection of RNA from corpses. We used Ebola virus–infected macaques to model humans who died of Ebola virus disease. Viable virus was isolated

  2. Hepatitis A virus antibody

    International Nuclear Information System (INIS)

    A description is presented of a radioimmunoassay designed to prove the presence of the antibody against the hepatitis A virus (HA Ab, anti-Ha) using an Abbott HAVAB set. This proof as well as the proof of the antibody against the nucleus of the hepatitis B virus is based on competition between a normal antibody against hepatitis A virus and a 125I-labelled antibody for the binding sites of a specific antigen spread all over the surface of a tiny ball; this is then indirect proof of the antibody under investigation. The method is described of reading the results from the number of impulses per 60 seconds: the higher the titre of the antibody against the hepatitis A virus in the serum examined, the lower the activity of the specimen concerned. The rate is reported of incidence of the antibody against the hepatitis A virus in a total of 68 convalescents after hepatitis A; the antibody was found in 94.1%. The immunoglobulin made from the convalescents' plasma showed the presence of antibodies in dilutions as high as 1:250 000 while the comparable ratio for normal immunoglobulin Norga was only 1:2500. Differences are discussed in the time incidence of the antibodies against the hepatitis A virus, the antibodies against the surface antigen of hepatitis B, and the antibody against the nucleus of the hepatitis V virus. (author)

  3. Archaeal virus-host interactions

    OpenAIRE

    Quax, T.E.F.

    2013-01-01

      The work presented in this thesis provides novel insights in several aspects of the molecular biology of archaea, bacteria and their viruses. Three fundamentally different groups of viruses are associated with the three domains of life. Archaeal viruses are characterized by a particularly high morphological and genetic diversity. Some archaeal viruses, such as Sulfolobus islandicus rod-shaped virus 2 (SIRV2), have quite remarkable infection cycles. As described in Chapter 1, infection ...

  4. Viruses in reptiles

    Directory of Open Access Journals (Sweden)

    Ariel Ellen

    2011-09-01

    Full Text Available Abstract The etiology of reptilian viral diseases can be attributed to a wide range of viruses occurring across different genera and families. Thirty to forty years ago, studies of viruses in reptiles focused mainly on the zoonotic potential of arboviruses in reptiles and much effort went into surveys and challenge trials of a range of reptiles with eastern and western equine encephalitis as well as Japanese encephalitis viruses. In the past decade, outbreaks of infection with West Nile virus in human populations and in farmed alligators in the USA has seen the research emphasis placed on the issue of reptiles, particularly crocodiles and alligators, being susceptible to, and reservoirs for, this serious zoonotic disease. Although there are many recognised reptilian viruses, the evidence for those being primary pathogens is relatively limited. Transmission studies establishing pathogenicity and cofactors are likewise scarce, possibly due to the relatively low commercial importance of reptiles, difficulties with the availability of animals and permits for statistically sound experiments, difficulties with housing of reptiles in an experimental setting or the inability to propagate some viruses in cell culture to sufficient titres for transmission studies. Viruses as causes of direct loss of threatened species, such as the chelonid fibropapilloma associated herpesvirus and ranaviruses in farmed and wild tortoises and turtles, have re-focused attention back to the characterisation of the viruses as well as diagnosis and pathogenesis in the host itself. 1. Introduction 2. Methods for working with reptilian viruses 3. Reptilian viruses described by virus families 3.1. Herpesviridae 3.2. Iridoviridae 3.2.1 Ranavirus 3.2.2 Erythrocytic virus 3.2.3 Iridovirus 3.3. Poxviridae 3.4. Adenoviridae 3.5. Papillomaviridae 3.6. Parvoviridae 3.7. Reoviridae 3.8. Retroviridae and inclusion body disease of Boid snakes 3.9. Arboviruses 3.9.1. Flaviviridae 3

  5. VHS virus - present situation

    DEFF Research Database (Denmark)

    Olesen, Niels Jørgen; Skall, Helle Frank

    worldwide distribution of the disease will be given. Virus evolution: Recent studies indicate that only a few amino acid changes in the structural proteins of VHSV can change the virulence patterns significantly, thereby coming closer to assessing the risk of none to low virulent viruses becoming high...... virulent. Virulence factors both depend on the ability of VHSV to enter a cell and on the speed and efficiency of virus replication in the cells. Apparently the viral nucleocapsid protein plays a very important role for the later and seems to be the target for determination of a virulence marker....

  6. VHS virus - present situation

    DEFF Research Database (Denmark)

    Skall, Helle Frank; Olesen, Niels Jørgen

    2015-01-01

    worldwide distribution of the disease will be given. Virus evolution: Recent studies indicate that only a few amino acid changes in the structural proteins of VHSV can change the virulence patterns significantly, thereby coming closer to assessing the risk of none to low virulent viruses becoming high...... virulent. Virulence factors both depend on the ability of VHSV to enter a cell and on the speed and efficiencyof virus replication in the cells. Apparently the viral nucleocapsid protein plays a very important role for the later and seems to be the target for determination of a virulence marker....

  7. Therapeutics and vaccines against chikungunya virus.

    Science.gov (United States)

    Ahola, Tero; Couderc, Therese; Courderc, Therese; Ng, Lisa F P; Hallengärd, David; Powers, Ann; Lecuit, Marc; Esteban, Mariano; Merits, Andres; Roques, Pierre; Liljeström, Peter

    2015-04-01

    Currently, there are no licensed vaccines or therapies available against chikungunya virus (CHIKV), and these were subjects discussed during a CHIKV meeting recently organized in Langkawi, Malaysia. In this review, we chart the approaches taken in both areas. Because of a sharp increase in new data in these fields, the present paper is complementary to previous reviews by Weaver et al. in 2012 and Kaur and Chu in 2013 . The most promising antivirals so far discovered are reviewed, with a special focus on the virus-encoded replication proteins as potential targets. Within the vaccines in development, our review emphasizes the various strategies in parallel development that are unique in the vaccine field against a single disease. PMID:25897811

  8. Hepatitis C virus host cell interactions uncovered

    DEFF Research Database (Denmark)

    Gottwein, Judith; Bukh, Jens

    2007-01-01

      Insights into virus-host cell interactions as uncovered by Randall et al. (1) in a recent issue of PNAS further our understanding of the hepatitis C virus (HCV) life cycle, persistence, and pathogenesis and might lead to the identification of new therapeutic targets. HCV persistently infects 180...... million individuals worldwide, causing chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. The only approved treatment, combination therapy with IFN- and ribavirin, targets cellular pathways (2); however, a sustained virologic response is achieved only in approximately half of the patients...... treated. Therefore, there is a pressing need for the identification of novel drugs against hepatitis C. Although most research focuses on the development of HCV-specific antivirals, such as protease and polymerase inhibitors (3), cellular targets could be pursued and might allow the development of broad...

  9. Virus como inductores de neoplasias cutáneas Viruses as agents inducing cutaneous neoplasms

    Directory of Open Access Journals (Sweden)

    Francisco Bravo Puccio

    2013-03-01

    Full Text Available El rol oncogénico de los virus en las neoplasias cutáneas es conocido por el hombre desde hace más de un siglo, cuando se atribuía el origen de la verruga vulgar al virus papiloma humano (VPH. En la actualidad, las neoplasias inducidas por virus pueden agruparse en tumores sólidos y procesos linfoproliferativos. Destacan entre los primeros el VPH, del cual ahora conocemos numerosos serotipos, cada uno vinculado a una neoplasia específica, el herpesvirus humano tipo 8 que produce el sarcoma de Kaposi y el poliomavirus vinculado al carcinoma de Merkel. Entre los procesos linfoproliferativos debemos mencionar al virus linfotrópico de células T humanas tipo 1 (HTLV-1 responsable de los linfomas de células T, en los cuales el compromiso cutáneo es inespecífico, con un amplio espectro de presentaciones clínicas y, que por consiguiente, plantean un reto para el diagnóstico diferencial. En este grupo también se encuentra el virus Epstein Barr vinculado a los linfomas nasales de Células NK/T y a los linfomas tipo Hidroa, de reciente descripción. En esta era en la que lo genético y lo molecular priman en las investigaciones en cáncer, no podemos dejar de lado el concepto de neoplasia como resultado de la infección por un agente viral, lo que abre una nueva veta de posibilidades de tratamiento anticanceroso basado en medicamentos antiviralesThe oncogenic role of viruses in cutaneous neoplasms has been known by humankind for more than a century, when the origin of the common wart, or verruca vulgaris, was attributed to the human papilloma virus (HPV. Currently, virus-induced cutaneous neoplasms may be grouped into solid tumors and lymphoproliferative disorders. HPV, from which various serotypes are now known, each being linked to a specific neoplasm, the human herpes virus type 8 producing Kaposi sarcoma, and the Merkel cell polyomavirus, highlight among the first group. Regarding the lymphoproliferative disorders, we should mention the

  10. Zika Virus Fact Sheet

    Science.gov (United States)

    ... medical care and advice. There is currently no vaccine available. Prevention Mosquito bites Protection against mosquito bites is a key measure to prevent Zika virus infection. This can be done by wearing ...

  11. Ebola (Ebola Virus Disease)

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Ebola (Ebola Virus Disease) Note: Javascript is disabled or is ... Tweet Share Compartir CDC's Ongoing Work to Contain Ebola in West Africa The Road to Zero: CDC’s ...

  12. Hepatitis G virus

    Institute of Scientific and Technical Information of China (English)

    Vasiliy Ivanovich Reshetnyak; Tatiana Igorevna Karlovich; Ljudmila Urievna Ilchenko

    2008-01-01

    A number of new hepatitis viruses (G,TT,SEN) were discovered late in the past century.We review the data available in the literature and our own findings suggesting that the new hepatitis G virus (HGV),disclosed in the late 1990s,has been rather well studied.Analysis of many studies dealing with HGV mainly suggests the lymphotropicity of this virus.HGV or GBV-C has been ascertained to influence course and prognosis in the HIV-infected patient.Until now,the frequent presence of GBV-C in coinfections,hematological diseases,and biliary pathology gives no grounds to determine it as an "accidental tourist" that is of no significance.The similarity in properties of GBV-C and hepatitis C virus (HCV) offers the possibility of using HGV,and its induced experimental infection,as a model to study hepatitis C and to develop a hepatitis C vaccine.

  13. [Zika, a neurotropic virus?].

    Science.gov (United States)

    Del Carpio-Orantes, Luis

    2016-01-01

    In this paper, the neurotropism potential Zika virus is discussed, by comparison with viruses both RNA and DNA are neurotropic known, also it is said that compared with the new viruses that have affected the Americas, as the chikungunya, Zika has shown great affinity by brain tissue, manifested by a high incidence of acute neurological conditions, such as Guillain-Barré syndrome, among others, as well as the reported incidence of microcephaly that is abnormally high compared with the previous incidence, which, in a stillborn subject necropsied significant alterations demonstrated in brain tissue, identifying viral material and live virus in the fetoplacental complex, and demonstrating the impact both white matter and gray matter as well as basal ganglia, corpus callosum, ventricles and spinal cord, which could explain the microcephaly that concerns him. Although not a direct cause-effect relationship is demonstrated, however current evidence supports that relationship, hoping to be supported scientifically. PMID:27197113

  14. Simian Immunodeficiency Virus Retropepsin

    Czech Academy of Sciences Publication Activity Database

    Konvalinka, Jan; Kräusslich, H. G.

    Amsterdam: Academic Press, 2013 - (Salvesen, G.), s. 204-207 ISBN 978-0-12-382219-2 Institutional support: RVO:61388963 Keywords : simian immunodeficiency virus * retropepsin * protease Subject RIV: CE - Biochemistry

  15. Ebola Virus Disease

    Science.gov (United States)

    ... long as their blood contains the virus. Sexual transmission More surveillance data and research are needed on the risks of sexual transmission, and particularly on the prevalence of viable and ...

  16. Zika Virus Outside Africa

    OpenAIRE

    Hayes, Edward B

    2009-01-01

    Zika virus (ZIKV) is a flavivirus related to yellow fever, dengue, West Nile, and Japanese encephalitis viruses. In 2007 ZIKV caused an outbreak of relatively mild disease characterized by rash, arthralgia, and conjunctivitis on Yap Island in the southwestern Pacific Ocean. This was the first time that ZIKV was detected outside of Africa and Asia. The history, transmission dynamics, virology, and clinical manifestations of ZIKV disease are discussed, along with the possibility for diagnostic ...

  17. Simian Varicella Virus Pathogenesis

    OpenAIRE

    Mahalingam, Ravi; Messaoudi, Ilhem; Gilden, Don

    2010-01-01

    Because varicella zoster virus (VZV) is an exclusively human pathogen, the development of an animal model is necessary to study pathogenesis, latency, and reactivation. The pathological, virological, and immunological features of simian varicella virus (SVV) infection in nonhuman primates are similar to those of VZV infection in humans. Both natural infection of cynomolgus and African green monkeys as well as intrabronchial inoculation of rhesus macaques with SVV provide the most useful model...

  18. Respiratory Syncytial Virus (RSV)

    Centers for Disease Control (CDC) Podcasts

    2013-02-04

    Respiratory Syncytial Virus, or RSV, causes cold-like symptoms but can be serious for infants and older adults. In this podcast, CDC’s Dr. Eileen Schneider discusses this common virus and offers tips to prevent its spread.  Created: 2/4/2013 by National Center for Immunization and Respiratory Diseases (NCIRD), Division of Viral Diseases (DVD).   Date Released: 2/13/2013.

  19. How do Viruses Attack Anti-Virus Programs

    OpenAIRE

    Mishra, Umakant

    2013-01-01

    As the anti-viruses run in a trusted kernel level any loophole in the anti-virus program can enable attackers to take full control over the computer system and steal data or do serious damages. Hence the anti-virus engines must be developed with proper security in mind. The ant-virus should be able to any type of specially created executable files, compression packages or documents that are intentionally created to exploit the anti-virus weakness. Viruses are present in almost every system ev...

  20. Volver al desarrollo

    Directory of Open Access Journals (Sweden)

    Jaime Ornelas Delgado

    2012-01-01

    Full Text Available El desarrollo, categoría empleada para expresar el crecimiento económico, surge durante la "guerra fría" y si bien fue propuesta por teóricos metropolitanos, se asumió en Latinoamérica como parte de los instrumentos disponibles para lograr el crecimiento y alternativa al socialismo. El desarrollo mantuvo su vigencia entre 1945 y 1975, cuando al advenimiento del neoliberalismo fue paulatinamente retirado de la agenda de las preocupaciones nacionales e internacionales. Al iniciarse el siglo xxi, el fracaso del mercado autorregulado trajo de nueva cuenta al debate los problemas del desarrollo, lo que obliga a revisarlo críticamente, tanto como al concepto mismo para mostrar su carácter colonial, si se quiere construir caminos ajenos al neoliberal y superar los problemas que han hecho de Latinoamérica una de las regiones más desiguales del mundo.

  1. Virus templated metallic nanoparticles

    Science.gov (United States)

    Aljabali, Alaa A. A.; Barclay, J. Elaine; Lomonossoff, George P.; Evans, David J.

    2010-12-01

    Plant viruses are considered as nanobuilding blocks that can be used as synthons or templates for novel materials. Cowpea mosaic virus (CPMV) particles have been shown to template the fabrication of metallic nanoparticles by an electroless deposition metallization process. Palladium ions were electrostatically bound to the virus capsid and, when reduced, acted as nucleation sites for the subsequent metal deposition from solution. The method, although simple, produced highly monodisperse metallic nanoparticles with a diameter of ca. templated particles were prepared with cobalt, nickel, iron, platinum, cobalt-platinum and nickel-iron.Plant viruses are considered as nanobuilding blocks that can be used as synthons or templates for novel materials. Cowpea mosaic virus (CPMV) particles have been shown to template the fabrication of metallic nanoparticles by an electroless deposition metallization process. Palladium ions were electrostatically bound to the virus capsid and, when reduced, acted as nucleation sites for the subsequent metal deposition from solution. The method, although simple, produced highly monodisperse metallic nanoparticles with a diameter of ca. templated particles were prepared with cobalt, nickel, iron, platinum, cobalt-platinum and nickel-iron. Electronic supplementary information (ESI) available: Additional experimental detail, agarose gel electrophoresis results, energy dispersive X-ray spectra, ζ-potential measurements, dynamic light scattering data, nanoparticle tracking analysis and an atomic force microscopy image of Ni-CPMV. See DOI: 10.1039/c0nr00525h

  2. Jalta als Heterotopie

    OpenAIRE

    Schmid, Ulrich

    2013-01-01

    Jalta ist ein Ort, der sich im europäischen Kulturgedächtnis sowohl historisch als auch literarisch als "radikal anderer Ort" - als " Heterotopie" - festgesetzt hat. An der Konferenz von Jalta wurde 1945 eine neue Weltordnung geschaffen in der Hoffnung auf eine bessere Welt. Auch in den Werken von Anton ?echov, vor allem in denjenigen, die in Jalta entstanden sind, wird von einer anderen und besseren Welt geträumt.

  3. Pornographie als Metapher

    Directory of Open Access Journals (Sweden)

    Caroline Schubarth

    2010-02-01

    Full Text Available In diesem Artikel werden unterschiedliche metaphorische Verwendungen des Pornografiebegriffs und deren Implikationen untersucht. Während die Existenz von Pornografie der feministischen Anti-Porno-Bewegung als Erklärung für die anhaltende Diskriminierung von Frauen in westlichen Gesellschaften dient, nutzen rechtskonservative Kräfte den Pornografievorwurf als Rechtfertigung für die Zensur von als deviant empfundenen Identitäten und sexuellen Praktiken.

  4. Pornographie als Metapher

    OpenAIRE

    Caroline Schubarth

    2010-01-01

    In diesem Artikel werden unterschiedliche metaphorische Verwendungen des Pornografiebegriffs und deren Implikationen untersucht. Während die Existenz von Pornografie der feministischen Anti-Porno-Bewegung als Erklärung für die anhaltende Diskriminierung von Frauen in westlichen Gesellschaften dient, nutzen rechtskonservative Kräfte den Pornografievorwurf als Rechtfertigung für die Zensur von als deviant empfundenen Identitäten und sexuellen Praktiken.This article...

  5. Sobre Al-Qarmuniyya

    OpenAIRE

    Fierro Bello, María Isabel

    1990-01-01

    [ES] En dos tradiciones escatológicas relativas a al-Andalus y recogidas por 'Abd al-Malik h Haha en su Ta'rg se hace referencia a Carmona. La mención de esta ciudad en dichas tradiciones debe ser puesta en relación con dos episodios de la historia de al-Andalus que tuvieron lugar antes de la muerte de Ibn Habib: la batalla de Carmona en época de 'Abd al-Rahman y la concentración de tropas omeyas en el mismo lugar durante el ataque normando en los años 229-30/844-5.

  6. Avian Influenza A Virus Infections in Humans

    Science.gov (United States)

    ... Past Newsletters Avian Influenza A Virus Infections in Humans Language: English Español Recommend on Facebook Tweet ... A Viruses Avian Influenza A Virus Infections in Humans Although avian influenza A viruses usually do not ...

  7. A New Definition of Virus

    Institute of Scientific and Technical Information of China (English)

    HE Hongjun; CAO Sihua; LUO Li; FENG Tao; PAN Li; ZOU Zhiji

    2006-01-01

    Security experts have not formally defined the distinction between viruses and normal programs. The paper takes user's intension as the criteria for malice, gives a formal definition of viruses that aim at stealing or destroying files, and proposes an algorithm to detect virus correctly. Compared with traditional definitions, this new definition is easy to understand, covers more malwares, adapts development of virus technology, and defines virus on the spot. The paper has also analyzed more than 250 real viruses and finds that they are all in the domain of the new definition, this implies that the new definition has great practical significance.

  8. [Zika virus infection during pregnancy].

    Science.gov (United States)

    Picone, O; Vauloup-Fellous, C; D'Ortenzio, E; Huissoud, C; Carles, G; Benachi, A; Faye, A; Luton, D; Paty, M-C; Ayoubi, J-M; Yazdanpanah, Y; Mandelbrot, L; Matheron, S

    2016-05-01

    A Zika virus epidemic is currently ongoing in the Americas. This virus is linked to congenital infections with potential severe neurodevelopmental dysfunction. However, incidence of fetal infection and whether this virus is responsible of other fetal complications are still unknown. National and international public health authorities recommend caution and several prevention measures. Declaration of Zika virus infection is now mandatory in France. Given the available knowledge on Zika virus, we suggest here a review of the current recommendations for management of pregnancy in case of suspicious or infection by Zika virus in a pregnant woman. PMID:27079865

  9. Caracterización de la neoplasia intraepitelial cervical inducida por el virus del papiloma humano en pacientes seropositivas a virus de la inmunodeficiencia humana

    OpenAIRE

    Rodríguez Bravo, Tomás Carlos

    2013-01-01

    [ES] Hemos pretendido caracterizar el comportamiento y la respuesta al tratamiento antirretroviral de las lesiones cervicales producidas por el virus del papiloma humano (HPV) en las pacientes infectadas también por el virus de la inmunodeficiencia adquirida (VIH). Se trata de de un estudio observacional y prospectivo, en el que la selección de la muestra procede del Servicio de enfermedades infecciosas del Complejo Hospitalario de Salamanca. Las pacientes fueron remitidas para control gin...

  10. Antiviral Therapy Reduces Viral Burden but Does Not Prevent Thymic Involution in Young Cats Infected with Feline Immunodeficiency Virus

    OpenAIRE

    Hayes, Kathleen A.; Phipps, Andrew J.; Francke, Sabine; Mathes, Lawrence E.

    2000-01-01

    The thymus is a major target organ in human immunodeficiency virus type 1 (HIV-1)-infected children and feline immunodeficiency virus (FIV)-infected young cats (G. A. Dean and N. C. Pedersen, J. Virol. 72:9436–9440, 1998; J. L. Heeney, Immunol. Today 16:515–520, 1995; S. M. Schnittman et al., Proc. Natl. Acad. Sci. USA 87:7727–7731, 1990; T. A. Seemayer et al., Hum. Pathol. 15:469–474, 1984; H.-J. Shuurn et al., Am. J. Pathol. 134:1329–1338, 1989; J. C. Woo et al., J. Virol. 71:8632–8641, 199...

  11. Isolation of porcine reproductive and respiratory syndrome (PRRS) virus in a Danish swine herd and experimental infection of pregnant gilts with the virus

    DEFF Research Database (Denmark)

    Bøtner, Anette; Nielsen, Jens; Bille-Hansen, Vivi

    The first case of porcine reproductive and respiratory syndrome (PRRS) in Denmark was diagnosed in March 1992 by the detection of specific antibodies against PRRS virus in serum samples originating from sows in a herd located on the island of Als. Subsequently, PRRS virus was isolated from a 200...... infection was demonstrated by the re-isolation of PRRS virus from approximately 45% of the piglets from the experimentally infected gilts. However, the experimental infection produced no significant reproductive disorders or other clinical signs. At autopsy, histopathological examination revealed slight......-sow farrow-to-finish herd with clinical signs consistent with PRRS. The virus was isolated by inoculation of pleural fluid from a stillborn piglet onto porcine pulmonary alveolar macrophages. The isolate was identified as PRRS virus by staining with a specific antiserum. By electron microscopy, the...

  12. Hepatitis viruses: Changing patterns of human disease

    OpenAIRE

    Purcell, R H

    1994-01-01

    Viral hepatitis is a disease of antiquity, but evidence for more than one etiologic agent has been recognized only since the 1940s, when two viruses (hepatitis A virus and hepatitis B virus) were thought to account for all disease. In the past 20 years, three additional hepatitis agents (hepatitis C virus, hepatitis D virus, and hepatitis E virus) have been discovered, and there is evidence for at least one additional virus. Each of the five recognized hepatitis viruses belongs to a different...

  13. Global Bifurcation of a Novel Computer Virus Propagation Model

    OpenAIRE

    2014-01-01

    In a recent paper by J. Ren et al. (2012), a novel computer virus propagation model under the effect of the antivirus ability in a real network is established. The analysis there only partially uncovers the dynamics behaviors of virus spread over the network in the case where around bifurcation is local. In the present paper, by mathematical analysis, it is further shown that, under appropriate parameter values, the model may undergo a global B-T bifurcation, and the curves of saddle-node bif...

  14. Al-Anon/Alateen

    Science.gov (United States)

    Find an Al-Anon meeting Español | Français 2018 Int'l Convention Home About Group Meetings What If I'm not ready ... a meeting? What can I expect at an Al-Anon meeting? What was my first meeting like? ...

  15. Effects of hydrography on the distribution of bacteria and virus in Cochin Estuary, India

    Digital Repository Service at National Institute of Oceanography (India)

    Parvathi, A.; Jasna, V.; Jina, S.; Jayalakshmy, K.V.; Lallu, K.R.; Madhu, N.V.; Muraleedharan, K.R.; NaveenKumar, K.R.; Balachandran, K.K.

    indicating the complex nature of the system (Ramamirtham and Muthusamy, 1986; Balachandran et al., 2008). Importantly, the CE has undergone drastic transformations over the past five decades and the increased microbial activity has transformed it from... an autotrophic to a highly heterotrophic system (Thottathil et al., 2008a; Gupta et al., 2009). The viruses play a crucial role in lysing bacterial and phytoplankton cells to release the dissolved organic matter and thereby increasing the community respiration...

  16. Molecular evolution of human immunodeficiency virus env in humans and monkeys: similar patterns occur during natural disease progression or rapid virus passage.

    Science.gov (United States)

    Hofmann-Lehmann, Regina; Vlasak, Josef; Chenine, Agnès-Laurence; Li, Pei-Lin; Baba, Timothy W; Montefiori, David C; McClure, Harold M; Anderson, Daniel C; Ruprecht, Ruth M

    2002-05-01

    Neonatal rhesus macaque 95-3 was inoculated with nonpassaged simian-human immunodeficiency virus strain SHIV-vpu(+), which encodes env of the laboratory-adapted human immunodeficiency virus (HIV) strain IIIB and is considered nonpathogenic. CD4(+) T-cell counts dropped to V. Narayan et al., Virology 256:54-63, 1999; R. Raghavan et al., Brain Pathol. 7:851-861, 1997; E. B. Stephens et al., Virology 231:313-321, 1997). Thus, HIV-IIIB env evolved similarly in three different species; this selection occurred in chronically infected individuals during disease progression as well as after rapid virus passage. We postulate that evolutionary pressure led to the outgrowth of more aggressive viral variants in all three species. PMID:11967343

  17. Pathogenesis of Alfalfa mosaic virus in Soybean (Glycine max) and Expression of Chimeric Rabies Peptide in Virus-Infected Soybean Plants.

    Science.gov (United States)

    Fleysh, N; Deka, D; Drath, M; Koprowski, H; Yusibov, V

    2001-10-01

    ABSTRACT Infection of soybean (Glycine max) plants inoculated with particles of Alfalfa mosaic virus (AlMV) isolate 425 at 12 days after germination was monitored throughout the life cycle of the plant (vegetative growth, flowering, seed formation, and seed maturation) by western blot analysis of tissue samples. At 8 to 10 days after inoculation, the upper uninoculated leaves showed symptoms of virus infection and accumulation of viral coat protein (CP). Virus CP was detectable in leaves, stem, roots, seedpods, and seed coat up to 45 days postinoculation (dpi), but only in the seedpod and seed coat at 65 dpi. No virus accumulation was detected in embryos and cotyledons at any time during infection, and no seed transmission of virus was observed. Soybean plants inoculated with recombinant AlMV passaged from upper uninoculated leaves of infected plants showed accumulation of full-length chimeric AlMV CP containing rabies antigen in systemically infected leaves and seed coat. These results suggest the potential usefulness of plants and plant viruses as vehicles for producing proteins of biomedical importance in a safe and inexpensive manner. Moreover, even the soybean seed coat, treated as waste tissue during conventional processing for oil and other products, may be utilized for the expression of value-added proteins. PMID:18944120

  18. Molecular epidemiology of respiratory viruses in virus-induced asthma

    OpenAIRE

    HiroyukiTsukagoshi; TaiseiIshioka

    2013-01-01

    Acute respiratory illness (ARI) due to various viruses is not only the most common cause of upper respiratory infection in humans but is also a major cause of morbidity and mortality, leading to diseases such as bronchiolitis and pneumonia. Previous studies have shown that respiratory syncytial virus (RSV), human rhinovirus (HRV), human metapneumovirus (HMPV), human parainfluenza virus (HPIV), and human enterovirus (HEV) infections may be associated with virus-induced asthma. For example, it ...

  19. Recombinant Vaccinia Virus: Immunization against Multiple Pathogens

    Science.gov (United States)

    Perkus, Marion E.; Piccini, Antonia; Lipinskas, Bernard R.; Paoletti, Enzo

    1985-09-01

    The coding sequences for the hepatitis B virus surface antigen, the herpes simplex virus glycoprotein D, and the influenza virus hemagglutinin were inserted into a single vaccinia virus genome. Rabbits inoculated intravenously or intradermally with this polyvalent vaccinia virus recombinant produced antibodies reactive to all three authentic foreign antigens. In addition, the feasibility of multiple rounds of vaccination with recombinant vaccinia virus was demonstrated.

  20. New prevalence estimate of Torque Teno virus(TTV) infection in healthy population and patients with chronic viral hepatitis in Jiujiang, China

    Institute of Scientific and Technical Information of China (English)

    Jin; Peng; Yueyue; Fang; Xuesen; Zhao; Yihong; Peng

    2015-01-01

    <正>Dear Editor,Torque Teno virus(TTV)is a nonenveloped human DNA virus that was isolated from the serum of a patient with transfusion-transmitted hepatitis with unknown etiology in 1997(Nishizawa et al.,1997).TTV is the first human virus with a single-stranded circular DNA genome to be identified,and is recently classified as the Alphatorquevirus genus of the Anelloviridae family by the International Committee on Taxonomy of Viruses(ICTV)(King et al.,2011).TTV shows very high genetic

  1. Al - BASED CAST COMPOSITES

    Directory of Open Access Journals (Sweden)

    Rakesh Kumar Yadav

    2011-08-01

    Full Text Available The economy is very important feature nowadays in themarket. The researches are playing an important role inengineering field to increase the life of machine parts /components and decrease the cost. The compositematerials have the potential to replace widely used steeland aluminium due to their good characteristics withbetter performance. The Al-based composites have foundextensive applications in automobile industries andaerospace industries due to their increased stiffness,strength, thermal conductivity and wear resistanceproperties. A number of particulate phases have beenemployed in the Al-alloy matrix. The cast aluminiumceramicparticulate composites are finding applications inpistons, connecting rods, cylinder liner, engine cylinderblock, electrical contacts etc.The present investigation isbased on study of the effect of particulate phase on theSEM study, micro-hardness, elastic modulus, tensilestrength and the wear behaviour of Al-5 % SiC-7 % Fe,Al-10 % SiC-6 % Fe and Al-15 % SiC-5 % Fe composites.

  2. Caracterización molecular de virus emergentes patógenos de tomate y pimiento y virus persistentes de aguacate

    OpenAIRE

    Villanueva Montiel, Francisco

    2014-01-01

    La mayoría de virus de plantas han sido descubiertos por producir enfermedades en especies de interés agronómico. Estos virus están asociados al huésped en una relación a corto plazo: infectan la planta, se replican rápidamente causando síntomas, son transmitidos horizontalmente por contacto o mediante un vector biológico y finalmente se convierten en infecciones crónicas o provocan la muerte de su hospedador. Sin embargo, también existen virus que mantienen un estilo de vida persistente en l...

  3. Influenza and other respiratory viruses in three Central American countries

    Science.gov (United States)

    Laguna‐Torres, Victor A.; Sánchez‐Largaespada, José F.; Lorenzana, Ivette; Forshey, Brett; Aguilar, Patricia; Jimenez, Mirna; Parrales, Eduardo; Rodriguez, Francisco; García, Josefina; Jimenez, Ileana; Rivera, Maribel; Perez, Juan; Sovero, Merly; Rios, Jane; Gamero, María E.; Halsey, Eric S.; Kochel, Tadeusz J.

    2010-01-01

    Please cite this paper as: Laguna‐Torres et al. (2011) Influenza and other respiratory viruses in three Central American countries. Influenza and Other Respiratory Viruses 5(2), 123–134. Background  Despite the disease burden imposed by respiratory diseases on children in Central America, there is a paucity of data describing the etiologic agents of the disease. Aims  To analyze viral etiologic agents associated with influenza‐like illness (ILI) in participants reporting to one outpatient health center, one pediatric hospital, and three general hospitals in El Salvador, Honduras, and Nicaragua Material & Methods  Between August 2006 and April 2009, pharyngeal swabs were collected from outpatients and inpatients. Patient specimens were inoculated onto cultured cell monolayers, and viral antigens were detected by indirect and direct immunofluorescence staining. Results  A total of 1,756 patients were enrolled, of whom 1,195 (68.3%) were under the age of 5; and 183 (10.4%) required hospitalization. One or more viral agents were identified in 434 (24.7%) cases, of which 17 (3.9%) were dual infections. The most common viruses isolated were influenza A virus (130; 7.4% of cases), respiratory syncytial virus (122; 6.9%), adenoviruses (63; 3.6%), parainfluenza viruses (57; 3.2%), influenza B virus (47; 2.7% of cases), and herpes simplex virus 1 (22; 1.3%). In addition, human metapneumovirus and enteroviruses (coxsackie and echovirus) were isolated from patient specimens. Discussion  When compared to the rest of the population, viruses were isolated from a significantly higher percentage of patients age 5 or younger. The prevalence of influenza A virus or influenza B virus infections was similar between the younger and older age groups. RSV was the most commonly detected pathogen in infants age 5 and younger and was significantly associated with pneumonia (p < 0.0001) and hospitalization (p < 0.0001). Conclusion  Genetic analysis of influenza

  4. Ebola (Ebola Virus Disease): Transmission

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Ebola (Ebola Virus Disease) Note: Javascript is disabled or is ... message, please visit this page: About CDC.gov . Ebola (Ebola Virus Disease) About Ebola Questions & Answers 2014 ...

  5. Ebola (Ebola Virus Disease): Prevention

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Ebola (Ebola Virus Disease) Note: Javascript is disabled or is ... message, please visit this page: About CDC.gov . Ebola (Ebola Virus Disease) About Ebola Questions & Answers 2014 ...

  6. Ebola (Ebola Virus Disease): Treatment

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Ebola (Ebola Virus Disease) Note: Javascript is disabled or is ... message, please visit this page: About CDC.gov . Ebola (Ebola Virus Disease) About Ebola Questions & Answers 2014 ...

  7. Ebola (Ebola Virus Disease): Diagnosis

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Ebola (Ebola Virus Disease) Note: Javascript is disabled or is ... message, please visit this page: About CDC.gov . Ebola (Ebola Virus Disease) About Ebola Questions & Answers 2014 ...

  8. Special Issue: Honey Bee Viruses

    Directory of Open Access Journals (Sweden)

    Sebastian Gisder

    2015-10-01

    Full Text Available Pollination of flowering plants is an important ecosystem service provided by wild insect pollinators and managed honey bees. Hence, losses and declines of pollinating insect species threaten human food security and are of major concern not only for apiculture or agriculture but for human society in general. Honey bee colony losses and bumblebee declines have attracted intensive research interest over the last decade and although the problem is far from being solved we now know that viruses are among the key players of many of these bee losses and bumblebee declines. With this special issue on bee viruses we, therefore, aimed to collect high quality original papers reflecting the current state of bee virus research. To this end, we focused on newly discovered viruses (Lake Sinai viruses, bee macula-like virus, or a so far neglected virus species (Apis mellifera filamentous virus, and cutting edge technologies (mass spectrometry, RNAi approach applied in the field.

  9. Zika Virus Infection and Microcephaly

    OpenAIRE

    J Gordon Millichap

    2016-01-01

    A Task Force established by the Brazil Ministry of Health investigated the possible association of microcephaly with Zika virus infection during pregnancy and a registry for microcephaly cases among women suspected to have had Zika virus infection during pregnancy.

  10. Epstein-Barr virus test

    Science.gov (United States)

    ... medlineplus.gov/ency/article/003513.htm Epstein-Barr virus antibody test To use the sharing features on this page, please enable JavaScript. Epstein-Barr virus antibody test is a blood test to detect ...

  11. Virions at the gates: receptors and the host-virus arms race.

    Directory of Open Access Journals (Sweden)

    John M Coffin

    Full Text Available All viruses need to bind to specific receptor molecules on the surface of target cells to initiate infection. Virus-receptor binding is highly specific, and this specificity determines both the species and the cell type that can be infected by a given virus. In some well-studied cases, the virus-binding region on the receptor has been found to be unrelated to the receptor's normal cellular function. Resistance to virus infection can thus evolve by selection of mutations that alter amino acids in the binding region with minimal effect on normal function. This sort of positive selection can be used to infer the history of the host-virus "arms race" during their coevolution. In a new study, Demogines et al. use a combination of phylogenetic, structural, and virological analysis to infer the history and significance of positive selection on the transferrin receptor TfR1, a housekeeping protein required for iron uptake and the cell surface receptor for at least three different types of virus. The authors show that only two parts of the rodent TfR1 molecule have been subject to positive selection and that these correspond to the binding sites for two of these viruses-the mouse mammary tumor virus (a retrovirus and Machupo virus (an arenavirus. They confirmed this result by introducing the inferred binding site mutations into the wild-type protein and testing for receptor function. Related arenaviruses are beginning to spread in human populations in South America as the cause of often fatal hemorrhagic fevers, and, although Demogines et al. could find no evidence of TfR1 mutations in this region that might have been selected as a consequence of human infection, the authors identified one such mutation in Asian populations that affects infection with these viruses.

  12. Avian influenza viruses in humans.

    OpenAIRE

    Malik Peiris, J S

    2009-01-01

    Past pandemics arose from low pathogenic avian influenza (LPAI) viruses. In more recent times, highly pathogenic avian influenza (HPAI) H5N1, LPAI H9N2 and both HPAI and LPAI H7 viruses have repeatedly caused zoonotic disease in humans. Such infections did not lead to sustained human-to-human transmission. Experimental infection of human volunteers and seroepidemiological studies suggest that avian influenza viruses of other subtypes may also infect humans. Viruses of the H7 subtype appear to...

  13. Computer virus : design and detection

    OpenAIRE

    Arding, Petter; Hedelin, Hugo

    2014-01-01

    Computer viruses uses a few different techniques, with various intentions, toinfect files. However, what most of them have in common is that they wantto avoid detection by anti-malware software. To not get detected and stay unnoticed,virus creators have developed several methods for this. Anti-malwaresoftware is constantly trying to counter these methods of virus infections withtheir own detection-techniques. In this paper we have analyzed the differenttypes of viruses and their infection tec...

  14. Antigenic variants of rabies virus

    OpenAIRE

    Wiktor, TJ; Koprowski, H

    1980-01-01

    Antigenic variants of CVS-11 strain of rabies virus were selected after treatment of virus populations with monoclonal antibodies directed against the glycoprotein antigen of the virus. These variants resisted neutralization by the hybridoma antibody used for their selection. Two independently mutating antigenic sites could be distinguished when five variants were tested with nine hybridoma antibodies. The frequency of single epitope variants in a cloned rabies virus seed was approximately 1:...

  15. [Environmental factors in ALS].

    Science.gov (United States)

    Juntas-Morales, Raul; Pageot, Nicolas; Corcia, Philippe; Camu, William

    2014-05-01

    ALS is likely to be a disorder of multifactorial origin. Among all the factors that may increase the risk of ALS, environmental ones are being studied for many years, but in the recent years, several advances have pointed to a new interest in their potential involvement in the disease process, especially for the cyanotoxin BMAA. Food containing BMAA has been found on Guam, a well-known focus of ALS/parkinsonism/dementia and high levels of BMAA have been identified into the brain of these patients. The BMAA cyanotoxin is potentially ubiquitous and have also been found into the food of patients who died from ALS both in Europe and USA. BMAA can be wrongly integrated into the protein structure during mRNA traduction, competing with serine. This may induce abnormal protein folding and a subsequent cell death. Heavy metals, such as lead or mercury may be directly toxic for neuronal cells. Several works have suggested an increased risk of ALS in individuals chronically exposed to these metals. Exposure to pesticides has been suggested to be linked to an increased risk of developing ALS. The mechanism of their toxicity is likely to be mediated by paraoxonases. These proteins are in charge of detoxifying the organism from toxins, and particularly organophosphates. To date, there are insufficient scientific data to suggest that exposure to electromagnetic fields may increase the risk of having ALS. We are particularly missing longitudinal cohorts to demonstrate that risk. PMID:24703731

  16. Rhabdomyolysis Associated with Parainfluenza Virus

    Directory of Open Access Journals (Sweden)

    Miltiadis Douvoyiannis

    2013-01-01

    Full Text Available Influenza virus is the most frequently reported viral cause of rhabdomyolysis. A 7-year-old child is presented with rhabdomyolysis associated with parainfluenza type 2 virus. Nine cases of rhabdomyolysis associated with parainfluenza virus have been reported. Complications may include electrolyte disturbances, acute renal failure, and compartment syndrome.

  17. Archaeal virus-host interactions

    NARCIS (Netherlands)

    Quax, T.E.F.

    2013-01-01

      The work presented in this thesis provides novel insights in several aspects of the molecular biology of archaea, bacteria and their viruses. Three fundamentally different groups of viruses are associated with the three domains of life. Archaeal viruses are characterized by a particularly

  18. Protecting Your Computer from Viruses

    Science.gov (United States)

    Descy, Don E.

    2006-01-01

    A computer virus is defined as a software program capable of reproducing itself and usually capable of causing great harm to files or other programs on the same computer. The existence of computer viruses--or the necessity of avoiding viruses--is part of using a computer. With the advent of the Internet, the door was opened wide for these…

  19. Ipomoviruses: Squash vein yellowing virus, Cucumber vein yellowing virus, Cassava brown streak virus, and Ugandan cassava brown streak virus

    Science.gov (United States)

    Ipomoviruses including Squash vein yellowing virus, Cucumber vein yellowing virus and Cassava brown streak virus are currently causing significant economic impact on crop production in several regions of the world. Only recently have results of detailed characterization of their whitefly transmissi...

  20. THE BIOLOGY OF INFLUENZA VIRUSES

    OpenAIRE

    Bouvier, Nicole M.; Palese, Peter

    2008-01-01

    The influenza viruses are characterized by segmented, negative-strand RNA genomes requiring an RNA-dependent RNA polymerase of viral origin for replication. The particular structure of the influenza virus genome and function of its viral proteins enable antigenic drift and antigenic shift. These processes result in viruses able to evade the long-term adaptive immune responses in many hosts.

  1. Avian influenza virus RNA extraction

    Science.gov (United States)

    The efficient extraction and purification of viral RNA is critical for down-stream molecular applications whether it is the sensitive and specific detection of virus in clinical samples, virus gene cloning and expression, or quantification of avian influenza (AI) virus by molecular methods from expe...

  2. Detección del Virus Mosaico Amarillo de la Calabaza Zucchini (ZYMV) y su Coinfección con otros Virus en Cucurbitáceas Cultivadas y Plantas Silvestres en el Valle del Fuerte, Sinaloa, México

    OpenAIRE

    Rubén Félix-Gastélum; Marco Antonio Magallanes-Tapia; Jesús Méndez-Lozano; Hervé Huet; José Ángel Trigueros-Salmerón; Rosa María Longoria-Espinoza

    2007-01-01

    Las enfermedades de origen viral son el principal factor limitante en la producción de cucurbitáceas en el Valle del Fuerte, Sinaloa, México. El objetivo del presente estudio fue detectar al virus mosaico amarillo de la calabaza zucchini (ZYMV), virus mosaico de la sandía (WMV), virus mancha anular del papayo variante sandía (PRSV-W) y virus mosaico del pepino (CMV) mediante ELISA y RT-PCR. En el mes de noviembre predominaron ZYMV con 54.5% de incidencia y la mezcla ZYMV + PRSV-W con 36.4%; e...

  3. Elucidating virus uptake and fusion by single virus tracing

    OpenAIRE

    Schupp, Dorothee Carolin

    2012-01-01

    Viruses are known to cause many diseases, from the common cold and cold sores to more serious diseases such as the Ebola virus disease and AIDS. Viruses have evolved different strategies to enter and infect cells. In order to infect a cell, viruses have to overcome the cell membrane barrier to deliver their genome to the site of replication. Enveloped viruses can either fuse directly at the plasma membrane or with an endosomal membrane after endocytic uptake. In this work, I studied the early...

  4. Relationship of lychnis ringspot virus to barley stripe mosaic virus and poa semilatent virus.

    Science.gov (United States)

    Hunter, B G; Smith, J; Fattouh, F; Jackson, A O

    1989-01-01

    Barley stripe mosaic virus (BSMV), poa semilatent virus (PSLV), and lychnis ringspot virus (LRSV) have previously been assigned to the hordeivirus group because of similarities in their particle morphology, physicochemical properties and serological analyses. However, the serological relationships of the three viruses have not been determined by direct comparison. The present study evaluated the relatedness of these viruses by Western and dot immunoblotting and by nucleic acid hybridizations. Serological analyses of the coat proteins separated by gel electrophoresis and of intact virus particles bound to nitrocellulose membranes revealed that BSMV and PSLV are distantly related, but that they are more closely related to each other than to LRSV. The genomic RNAs of the viruses failed to cross-hybridize in northern hybridization tests conducted at different temperatures. These comparisons showed that BSMV, PSLV and LRSV are distinct viruses with little nucleotide sequence relatedness. Thus our data provide additional support for their inclusion as separate members of the hordeivirus group. PMID:2722469

  5. Research on computer virus database management system

    Science.gov (United States)

    Qi, Guoquan

    2011-12-01

    The growing proliferation of computer viruses becomes the lethal threat and research focus of the security of network information. While new virus is emerging, the number of viruses is growing, virus classification increasing complex. Virus naming because of agencies' capture time differences can not be unified. Although each agency has its own virus database, the communication between each other lacks, or virus information is incomplete, or a small number of sample information. This paper introduces the current construction status of the virus database at home and abroad, analyzes how to standardize and complete description of virus characteristics, and then gives the information integrity, storage security and manageable computer virus database design scheme.

  6. Zika virus: Indian perspectives

    Science.gov (United States)

    Mourya, Devendra T.; Shil, Pratip; Sapkal, Gajanan N.; Yadav, Pragya D.

    2016-01-01

    The emergence of Zika virus (ZiV), a mosquito borne Flavivirus like dengue (DEN) and chikungunya (CHIK), in Brazil in 2014 and its spread to various countries have led to a global health emergency. Aedes aegypti is the major vector for ZiV. Fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ZiV and there is abundance of Aedes mosquitoes. In this review, we focus on current global scenario, epidemiology, biology, diagnostic challenges and remedial measures for ZiVconsidering the Indian perspective. PMID:27487998

  7. [ZIKA--VIRUS INFECTION].

    Science.gov (United States)

    Velev, V

    2016-01-01

    This review summarizes the knowledge of the scientific community for Zika-virus infection. It became popular because of severe congenital damage causes of CNS in newborns whose mothers are infected during pregnancy, as well as the risk of pandemic distribution. Discusses the peculiarities of the biology and ecology of vectors--blood-sucking mosquitoes Aedes; stages in the spread of infection and practical problems which caused during pregnancy. Attention is paid to the recommendations that allow leading national and international medical organizations to deal with the threat Zika-virus infection. PMID:27509655

  8. Zika virus: Indian perspectives.

    Science.gov (United States)

    Mourya, Devendra T; Shil, Pratip; Sapkal, Gajanan N; Yadav, Pragya D

    2016-05-01

    The emergence of Zika virus (ZiV), a mosquito borne Flavivirus like dengue (DEN) and chikungunya (CHIK), in Brazil in 2014 and its spread to various countries have led to a global health emergency. Aedes aegypti is the major vector for ZiV. Fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ZiV and there is abundance of Aedes mosquitoes. In this review, we focus on current global scenario, epidemiology, biology, diagnostic challenges and remedial measures for ZiVconsidering the Indian perspective. PMID:27487998

  9. Sensing of RNA viruses

    DEFF Research Database (Denmark)

    Jensen, Søren; Thomsen, Allan Randrup

    2012-01-01

    Our knowledge regarding the contribution of the innate immune system in recognizing and subsequently initiating a host response to an invasion of RNA virus has been rapidly growing over the last decade. Descriptions of the receptors involved and the molecular mechanisms they employ to sense viral...... pathogen-associated molecular patterns have emerged in great detail. This review presents an overview of our current knowledge regarding the receptors used to detect RNA virus invasion, the molecular structures these receptors sense, and the involved downstream signaling pathways....

  10. Tenosinovitis por virus Chikungunya

    Directory of Open Access Journals (Sweden)

    Alfredo Seijo

    2014-12-01

    Full Text Available Se presenta a la consulta un hombre proveniente de la República Dominicana con una tenosinovitis del extensor del dedo medio derecho; en la convalecencia inmediata, segunda curva febril luego de 48 horas de permanecer asintomático de una enfermedad febril aguda, y marcada astenia, exantema pruriginoso, poliartralgias con impotencia funcional y rigidez articular generalizada. Los exámenes bioquímicos no aportaron datos de interés para el diagnóstico. La serología para virus dengue fue negativa. La detección de IgM y de anticuerpos neutralizantes para virus Chikungunya (CHIKV fueron positivos.

  11. Zika virus: Indian perspectives

    Directory of Open Access Journals (Sweden)

    Devendra T Mourya

    2016-01-01

    Full Text Available The emergence of Zika virus (ZiV, a mosquito borne Flavivirus like dengue (DEN and chikungunya (CHIK, in Brazil in 2014 and its spread to various countries have led to a global health emergency. Aedes aegypti is the major vector for ZiV. Fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ZiV and there is abundance of Aedes mosquitoes. In this review, we focus on current global scenario, epidemiology, biology, diagnostic challenges and remedial measures for ZiVconsidering the Indian perspective.

  12. Bovine Virus Diarrhea (BVD)

    OpenAIRE

    Hoar, Bruce R.

    2004-01-01

    Bovine virus diarrhea (BVD) is a complicated disease to discuss as it can result in a wide variety of disease problems from very mild to very severe. BVD can be one of the most devastating diseases cattle encounter and one of the hardest to get rid of when it attacks a herd. The viruses that cause BVD have been grouped into two genotypes, Type I and Type II. The disease syndrome caused by the two genotypes is basically the same, however disease caused by Type II infection is often more severe...

  13. Parainfluenza Virus 5 Expressing the G Protein of Rabies Virus Protects Mice after Rabies Virus Infection

    OpenAIRE

    Huang, Ying; Chen, Zhenhai; Huang, Junhua; Fu, Zhenfang; He, Biao

    2014-01-01

    Rabies remains a major public health threat around the world. Once symptoms appear, there is no effective treatment to prevent death. In this work, we tested a recombinant parainfluenza virus 5 (PIV5) strain expressing the glycoprotein (G) of rabies (PIV5-G) as a therapy for rabies virus infection: we have found that PIV5-G protected mice as late as 6 days after rabies virus infection. PIV5-G is a promising vaccine for prevention and treatment of rabies virus infection.

  14. Sociale media als leermiddel

    NARCIS (Netherlands)

    Rubens, Wilfred

    2012-01-01

    Rubens, W. (2012, 5 juni). Sociale media als leermiddel. Presentatie over het gebruik van sociale media binnen het onderwijs, verzorgd tijdens een ouderraadbijeenkomst van de Katholieke Scholengemeenschap Etten-Leur, Etten-Leur, Nederland.

  15. MDA ALS Caregiver's Guide

    Science.gov (United States)

    ... R.D., C.N.S.D., L.D.N. Nutritionist MDA/ALS Center at Vanderbilt University Medical Center ... neurologists, physiatrists, therapists (physical, occupa- tional, speech, respiratory), nutritionists, social work- ers, pulmonologists, gastroenterologists and medical equipment ...

  16. Regionale Herkunft als Bonus

    OpenAIRE

    Kratochvil, Dr. Ruth

    2004-01-01

    Der Bio-Sektor durchlebt bewegte Zeiten. Die biologische Landwirtschaft hat sich aus der Nische herausbewegt und dafür an „Besonderheiten“ eingebüßt. Diese müssen als Zusatznutzen wiedergefunden werden.

  17. Factores asociados al bajo peso al nacer

    OpenAIRE

    Georgina J Peraza Roque; Silvia de la C Pérez Delgado; Zoe de los A Figueroa Barreto

    2001-01-01

    El bajo peso al nacer (BPN) ha constituido un enigma en la ciencia a través de los tiempos. Múltiples son las investigaciones realizadas acerca de las causas que lo producen y las consecuencias que provoca. Su importancia no solo radica en lo que significa en la morbilidad y la mortalidad infantil, sino que estos niños tienen habitualmente múltiples problemas posteriores. El Programa para la Reducción del BPN señala que los niños nacidos con un peso inferior a los 2 500 g presentan riesgo de ...

  18. Occurrence of Deformed wing virus, Chronic bee paralysis virus and mtDNA variants in haplotype K of Varroa destructor mites in Syrian apiaries.

    Science.gov (United States)

    Elbeaino, Toufic; Daher-Hjaij, Nouraldin; Ismaeil, Faiz; Mando, Jamal; Khaled, Bassem Solaiman; Kubaa, Raied Abou

    2016-05-01

    A small-scale survey was conducted on 64 beehives located in four governorates of Syria in order to assess for the first time the presence of honeybee-infecting viruses and of Varroa destructor mites in the country. RT-PCR assays conducted on 192 honeybees (Apis mellifera L.) using virus-specific primers showed that Deformed wing virus (DWV) was present in 49 (25.5%) of the tested samples and Chronic bee paralysis virus (CBPV) in 2 (1.04%), whereas Acute bee paralysis virus, Sacbrood virus, Black queen cell virus and Kashmir bee virus were absent. Nucleotide sequences of PCR amplicons obtained from DWV and CBPV genomes shared 95-97 and 100% identity with isolates reported in the GenBank, respectively. The phylogenetic tree grouped the Syrian DWV isolates in one cluster, distinct from all those of different origins reported in the database. Furthermore, 19 adult V. destructor females were genetically analyzed by amplifying and sequencing four fragments in cytochrome oxidase subunit 1 (cox1), ATP synthase 6 (atp6), cox3 and cytochrome b (cytb) mitochondrial DNA (mtDNA) genes. Sequences of concatenated V. destructor mtDNA genes (2696 bp) from Syria were similar to the Korean (K) haplotype and were found recurrently in all governorates. In addition, two genetic lineages of haplotype K with slight variations (0.2-0.3%) were present only in Tartous and Al-Qunaitra governorates. PMID:26914360

  19. Prototype development of the integral-field unit for VIRUS

    Science.gov (United States)

    Kelz, Andreas; Bauer, Svend M.; Grupp, Frank; Hill, Gary J.; Popow, Emil; Palunas, Povilas; Roth, Martin M.; MacQueen, Phillip J.; Tripphahn, Ute

    2006-06-01

    VIRUS is a planned integral-field instrument for the Hobby-Eberly Telescope (HET). In order to achieve a large field-of-view and high grasp at reasonable costs, the approach is to replicate integral-field units (IFU) and medium sized spectrographs many times. The Astrophysical Institute Potsdam (AIP) contributes to VIRUS with the development and testing of the IFU prototype. While the overall project is presented by Hill et al.1, this paper describes the opto-mechanical design and the manufacture of the fiber-based IFU subsystem. The initial VIRUS development aims to produce a prototype and to measure its performance. Additionally, techniques will be investigated to allow industrial replication of the highly specific fiber-bundle layout. This will be necessary if this technique is to be applied to the next generation of even larger astronomical instrumentation.

  20. Neutralisation and binding of VHS virus by monovalent antibody fragments

    DEFF Research Database (Denmark)

    Cupit, P.M.; Lorenzen, Niels; Strachan, G.; Kemp, G.J.L.; Secombes, C.J.; Cunningham, C.

    We have previously reported the cloning and characterisation of the heavy and light chain variable domain genes encoding three monoclonal antibodies (Mabs) that bind viral haemorrhagic septicaemia virus (VHSV). Two of these antibodies, 3F1H10 and 3F1A2 both neutralised the virus though 3F1A2...... appeared to recognise a broader range of virus isolates. The variable domains of these two antibodies differ by only four residues (Lorenzen et al., 2000a. Fish Shellfish Immunol. 10, 129-142). To further study the mechanism of neutralisation, Fab fragments as well as a series of recombinant bacterial...... BIAcore analysis and found to correlate with the capacity of each molecule to neutralise DK-F1. These investigations, together with computer assisted molecular analysis of the theoretical influence of each mutation on antigen binding, led to the identification of a single mutation at position 35a in the...

  1. Partizipation als Ausnahmezustand

    OpenAIRE

    Korf, Benedikt

    2009-01-01

    In «Die Erfindung des Politischen» (1993) beschreibt Ulrich Beck Subpolitik als den Ort, an dem die Grenze zwischen Politik und Nichtpolitik verschwindet. Aus der zunehmenden Wirkkraft von Subpolitik erwächst die grundlegende Frage, mit welcher Legitimation diese Art von Subpolitik in die Sphäre der institutionalisierten Politik eindringt. Beck’s Subpolitik soll im Anschluss an theoretische Arbeiten von Chantal Mouffe und Giorgio Agamben als post-politische Aspiration bezeichnet werden, die e...

  2. Single Assay Detection of Acute Bee Paralysis Virus, Kashmir Bee Virus and Israeli Acute Paralysis Virus

    DEFF Research Database (Denmark)

    Francis, Roy Mathew; Kryger, Per

    2012-01-01

    A new RT-PCR primer pair designed to identify Acute Bee Paralysis Virus (ABPV), Kashmir Bee Virus (KBV) or Israeli Acute Bee Paralysis Virus (IAPV) of honey bees (Apis mellifera L.) in a single assay is described. These primers are used to screen samples for ABPV, KBV, or IAPV in a single RT...

  3. Viruses, Artificial Viruses and Virus-Based Structures for Biomedical Applications

    NARCIS (Netherlands)

    van Rijn, Patrick; Schirhagl, Romana

    2016-01-01

    Nanobiomaterials such as virus particles and artificial virus particles offer tremendous opportunities to develop new biomedical applications such as drug- or gene-delivery, imaging and sensing but also improve understanding of biological mechanisms. Recent advances within the field of virus-based s

  4. Small-RNA deep sequencing reveals Arctium tomentosum as a natural host of Alstroemeria virus X and a new putative Emaravirus.

    Directory of Open Access Journals (Sweden)

    Yaqi Bi

    Full Text Available BACKGROUND: Arctium species (Asteraceae are distributed worldwide and are used as food and rich sources of secondary metabolites for the pharmaceutical industry, e.g., against avian influenza virus. RNA silencing is an antiviral defense mechanism that detects and destroys virus-derived double-stranded RNA, resulting in accumulation of virus-derived small RNAs (21-24 nucleotides that can be used for generic detection of viruses by small-RNA deep sequencing (SRDS. METHODOLOGY/PRINCIPAL FINDINGS: SRDS was used to detect viruses in the biennial wild plant species Arctium tomentosum (woolly burdock; family Asteraceae displaying virus-like symptoms of vein yellowing and leaf mosaic in southern Finland. Assembly of the small-RNA reads resulted in contigs homologous to Alstroemeria virus X (AlsVX, a positive/single-stranded RNA virus of genus Potexvirus (family Alphaflexiviridae, or related to negative/single-stranded RNA viruses of the genus Emaravirus. The coat protein gene of AlsVX was 81% and 89% identical to the two AlsVX isolates from Japan and Norway, respectively. The deduced, partial nucleocapsid protein amino acid sequence of the emara-like virus was only 78% or less identical to reported emaraviruses and showed no variability among the virus isolates characterized. This virus--tentatively named as Woolly burdock yellow vein virus--was exclusively associated with yellow vein and leaf mosaic symptoms in woolly burdock, whereas AlsVX was detected in only one of the 52 plants tested. CONCLUSIONS/SIGNIFICANCE: These results provide novel information about natural virus infections in Acrtium species and reveal woolly burdock as the first natural host of AlsVX besides Alstroemeria (family Alstroemeriaceae. Results also revealed a new virus related to the recently emerged Emaravirus genus and demonstrated applicability of SRDS to detect negative-strand RNA viruses. SRDS potentiates virus surveys of wild plants, a research area underrepresented in

  5. Der Preis als Kaufbarriere?

    DEFF Research Database (Denmark)

    Mueller Loose, Simone

    2012-01-01

    Die Preispolitik hat eine herausragende Stellung im Marketing. Der Preis eines Produktes ist für Verbraucher ein direkter Kostenfaktor und für Unternehmen die Stellgröße im Mar-ketingmix, die den Umsatz am stärksten beeinflusst (vgl. Nieschlag et al. 2002). Wenn die Akzeptanz und der regelmäßige...... Verzehr gesunder Lebensmittel erhöht werden soll, ist es sowohl für die Verbraucherpolitik als auch für Lebensmittelproduzenten wichtig, den Ein-fluss des Preises auf die Kaufentscheidung zu verstehen. Aus Verbrauchersicht ist der Preis neben dem Geschmack die wichtigste Produkteigen-schaft beim Kauf von...... Lebensmitteln (vgl. Brunsø/Grunert 1998) und stellt eine Kaufbarrie-re für Lebensmittel mit gesundheitsfördernden Eigenschaften dar. Studien in den USA (vgl. Cassady et al. 2007), Frankreich (vgl. Drewnowski et al. 2004; Andrieu et al. 2005) und Deutschland (vgl. Karg et al. 2008) kommen übereinstimmend zu dem...

  6. Targeted modifications of foot-and-mouth disease virus; towards improved vaccine candidates

    DEFF Research Database (Denmark)

    Gullberg, Maria; Polacek, Charlotta; Bøtner, Anette;

    Foot-and-mouth disease virus (FMDV) is responsible for one of the most economically important diseases of farm animals (estimated annual costs are about US$10 billion globally). The virus is the prototypic Aphthovirus within the family Picornaviridae and has a positive sense RNA genome (ca. 8.3kb...... these into susceptible cells it is possible to rescue specifically altered FMDVs. We have used this approach to generate modified viruses that have particular properties; these studies can assist in the development of improved and safer vaccines to protect against FMDV. For example, we have made changes to the leader (L...... “self-tagged” virus particles that contain the VP1-2A precursor (Gullberg et al., 2013). This approach works for two of the most common FMDV serotypes (O and A) and offers the possibility of a single approach to purifying virus particles from different serotypes using reagents targeted to the conserved...

  7. Bat flight and zoonotic viruses

    Science.gov (United States)

    O'Shea, Thomas; Cryan, Paul M.; Cunningham, Andrew A.; Fooks, Anthony R.; Hayman, David T.S.; Luis, Angela D.; Peel, Alison J.; Plowright, Raina K.; Wood, James L.N.

    2014-01-01

    Bats are sources of high viral diversity and high-profile zoonotic viruses worldwide. Although apparently not pathogenic in their reservoir hosts, some viruses from bats severely affect other mammals, including humans. Examples include severe acute respiratory syndrome coronaviruses, Ebola and Marburg viruses, and Nipah and Hendra viruses. Factors underlying high viral diversity in bats are the subject of speculation. We hypothesize that flight, a factor common to all bats but to no other mammals, provides an intensive selective force for coexistence with viral parasites through a daily cycle that elevates metabolism and body temperature analogous to the febrile response in other mammals. On an evolutionary scale, this host–virus interaction might have resulted in the large diversity of zoonotic viruses in bats, possibly through bat viruses adapting to be more tolerant of the fever response and less virulent to their natural hosts.

  8. The FEFM Virus.

    Science.gov (United States)

    Cjelli, Dirk

    1994-01-01

    Discussion of human performance technology (HPT) interventions focuses on requests for solutions to problems that involve conflict between the FEFM ("Fix 'em for me") Virus and the WIIFM ("What's in it for me?") Requirement. Building a relationship of mutual trust and confidence with the client and demonstrating responsiveness are advocated. (LRW)

  9. Viruses of Haloarchaea

    Directory of Open Access Journals (Sweden)

    Alison W. S. Luk

    2014-11-01

    Full Text Available In hypersaline environments, haloarchaea (halophilic members of the Archaea are the dominant organisms, and the viruses that infect them, haloarchaeoviruses are at least ten times more abundant. Since their discovery in 1974, described haloarchaeoviruses include head-tailed, pleomorphic, spherical and spindle-shaped morphologies, representing Myoviridae, Siphoviridae, Podoviridae, Pleolipoviridae, Sphaerolipoviridae and Fuselloviridae families. This review overviews current knowledge of haloarchaeoviruses, providing information about classification, morphotypes, macromolecules, life cycles, genetic manipulation and gene regulation, and host-virus responses. In so doing, the review incorporates knowledge from laboratory studies of isolated viruses, field-based studies of environmental samples, and both genomic and metagenomic analyses of haloarchaeoviruses. What emerges is that some haloarchaeoviruses possess unique morphological and life cycle properties, while others share features with other viruses (e.g., bacteriophages. Their interactions with hosts influence community structure and evolution of populations that exist in hypersaline environments as diverse as seawater evaporation ponds, to hot desert or Antarctic lakes. The discoveries of their wide-ranging and important roles in the ecology and evolution of hypersaline communities serves as a strong motivator for future investigations of both laboratory-model and environmental systems.

  10. Viruses of haloarchaea.

    Science.gov (United States)

    Luk, Alison W S; Williams, Timothy J; Erdmann, Susanne; Papke, R Thane; Cavicchioli, Ricardo

    2014-01-01

    In hypersaline environments, haloarchaea (halophilic members of the Archaea) are the dominant organisms, and the viruses that infect them, haloarchaeoviruses are at least ten times more abundant. Since their discovery in 1974, described haloarchaeoviruses include head-tailed, pleomorphic, spherical and spindle-shaped morphologies, representing Myoviridae, Siphoviridae, Podoviridae, Pleolipoviridae, Sphaerolipoviridae and Fuselloviridae families. This review overviews current knowledge of haloarchaeoviruses, providing information about classification, morphotypes, macromolecules, life cycles, genetic manipulation and gene regulation, and host-virus responses. In so doing, the review incorporates knowledge from laboratory studies of isolated viruses, field-based studies of environmental samples, and both genomic and metagenomic analyses of haloarchaeoviruses. What emerges is that some haloarchaeoviruses possess unique morphological and life cycle properties, while others share features with other viruses (e.g., bacteriophages). Their interactions with hosts influence community structure and evolution of populations that exist in hypersaline environments as diverse as seawater evaporation ponds, to hot desert or Antarctic lakes. The discoveries of their wide-ranging and important roles in the ecology and evolution of hypersaline communities serves as a strong motivator for future investigations of both laboratory-model and environmental systems. PMID:25402735

  11. Viruses of the Archaea

    DEFF Research Database (Denmark)

    Basta, T.; Garrett, Roger Antony; Prangishvili,, David

    Double-stranded deoxyribonucleic acid (DNA) viruses that infect members of the third domain of life, the Archaea, are diverse and exceptional in both their morphotypes and their genomic properties. The majority of characterized species infect hyperthermophilic hosts and carry morphological features...

  12. The virus of management

    DEFF Research Database (Denmark)

    Kjær, Peter; Frankel, Christian

    2003-01-01

    concrete management knowledge and practice. The article isstructured as follows. After the introduction, we first develop the notion of organizational virus asinto an analytical approach. Second, we discern in the work of Frederick Taylor on scientificmanagement and Max Weber on bureaucracy, two quite...

  13. Viruses and febrile seizures

    NARCIS (Netherlands)

    Zeijl, J.H. van

    2004-01-01

    We conclude that viral infections are the main cause of febrile seizures, with an important role for influenza A, HHV-6 and HHV-7. We showed that several viral infections not only contribute to initial febrile seizures, but also to recurrences. Viruses could not be detected in the CSF of children wi

  14. Análisis del estado de inmunización de la población mayor de 65 años de Castilla y León frente al virus de la Gripe Aviar H5N1

    OpenAIRE

    Sanz Muñoz, Iván

    2013-01-01

    1- Los valores en la protección previa a la vacuna de los distintos virus gripales endémicos españoles está en consonancia con su nivel de incidencia y relevancia en las epidemias anuales. 2- Los niveles de pre-protección de los distintos virus están en función de los acontecimientos epidémicos y de la gestión vacunal de las temporadas anteriores. 3- Los niveles de protección derivados de la vacunación siguen con respecto a los niveles de protección pre-vacunal un patrón evo...

  15. Peptide-based candidate vaccine against respiratory syncytial virus.

    Science.gov (United States)

    Yusibov, Vidadi; Mett, Vadim; Mett, Valentina; Davidson, Carley; Musiychuk, Konstantin; Gilliam, Suzan; Farese, Ann; Macvittie, Thomas; Mann, Dean

    2005-03-18

    We engineered a 21-mer peptide representing amino acids 170-190 of the respiratory syncytial virus (RSV) G protein as a fusion with the Alfalfa mosaic virus (AlMV) coat protein (CP), produced recombinant AlMV particles presenting this peptide (VMR-RSV) on their surfaces and tested the immunogenicity in vitro in human dendritic cells and in vivo in non-human primates. Significant pathogen-specific immune responses were generated in both systems: (i) human dendritic cells armed with VMR-RSV generated vigorous CD4+ and CD8+ T cell responses; (ii) non-human primates that received these particles responded by mounting strong cellular and humoral immune responses. This approach may validate the use of a novel RSV vaccine delivery vehicle in humans. PMID:15755607

  16. Infección crónica por el VHC Chronic Hepatitis C virus infection

    OpenAIRE

    M. Iñarrairaegui; I. Elizalde; A. Martínez Echeverría; J.M. Zozaya; R. Beloqui; J. M. Martínez-Peñuela

    2004-01-01

    Tras la infección aguda por el virus de la hepatitis C (VHC), un porcentaje importante de pacientes no aclara el virus y desarrollan una hepatitis crónica C. Los síntomas, cuando existen, suelen ser inespecíficos. Aproximadamente un tercio de los pacientes presentan además manifestaciones extrahepáticas de la infección, debidas fundamentalmente al linfotropismo del virus C. De éstas destacan, por su clara asociación con el VHC, la crioglobulinemia mixta y la producción de autoanticuerpos (aut...

  17. Diversity of putative archaeal RNA viruses in metagenomic datasets of a yellowstone acidic hot spring.

    OpenAIRE

    Hongming WANG; Yu, Yongxin; Liu, Taigang; Pan, Yingjie; Yan, Shuling; Wang, Yongjie

    2015-01-01

    Two genomic fragments (5,662 and 1,269 nt in size, GenBank accession no. JQ756122 and JQ756123, respectively) of novel, positive-strand RNA viruses that infect archaea were first discovered in an acidic hot spring in Yellowstone National Park (Bolduc et al., 2012). To investigate the diversity of these newly identified putative archaeal RNA viruses, global metagenomic datasets were searched for sequences that were significantly similar to those of the viruses. A total of 3,757 associated read...

  18. Evolutionary relationship of alfalfa mosaic virus with cucumber mosaic virus and brome mosaic virus

    OpenAIRE

    Savithri, HS; Murthy, MRN

    1983-01-01

    The amino acid sequences of the non-structural protein (molecular weight 35,000; 3a protein) from three plant viruses - cucumber mosaic, brome mosaic and alfalfa mosaic have been systematically compared using the partial genomic sequences for these three viruses already available. The 3a protein of cucumber mosaic virus has an amino acid sequence homology of 33.7% with the corresponding protein of brome mosaic virus. A similar protein from alfalfa mosaic virus has a homology of 18.2% and 14.2...

  19. Modification of residual stress in Al-[AlBO]w/Al compound plate

    Institute of Scientific and Technical Information of China (English)

    姜传海; 吴建生; 王德尊

    2001-01-01

    The application of compound parts of Al-[AlBO]w/Al not only reduces the cost of the parts but also improves its properties. However, there is a large thermal residual stress between Al and [AlBO]w/Al, and it is harmful for practical application. From the theoretical analyses and experimental results, it was found that by the compressive pre-plastic deformation perpendicular to the interface between Al and [AlBO]w/Al, the interlayer residual stress of compound parts can be reduced, while the mechanical properties of compound parts can be improved.

  20. Molecular epidemiology of respiratory viruses in virus-induced asthma

    Directory of Open Access Journals (Sweden)

    Hiroyuki eTsukagoshi

    2013-09-01

    Full Text Available Acute respiratory illness (ARI due to various viruses is not only the most common cause of upper respiratory infection in humans but is also a major cause of morbidity and mortality, leading to diseases such as bronchiolitis and pneumonia. Previous studies have shown that respiratory syncytial virus (RSV, human rhinovirus (HRV, human metapneumovirus (HMPV, human parainfluenza virus (HPIV, and human enterovirus (HEV infections may be associated with virus-induced asthma. For example, it has been suggested that HRV infection is detected in the acute exacerbation of asthma and infection is prolonged. Thus it is believed that the main etiological cause of asthma is ARI viruses. Furthermore, the number of asthma patients in most industrial countries has greatly increased, resulting in a morbidity rate of around 10-15% of the population. However, the relationships between viral infections, host immune response, and host factors in the pathophysiology of asthma remain unclear. To gain a better understanding of the epidemiology of virus-induced asthma, it is important to assess both the characteristics of the viruses and the host defense mechanisms. Molecular epidemiology enables us to understand the pathogenesis of microorganisms by identifying specific pathways, molecules, and genes that influence the risk of developing a disease. However, the epidemiology of various respiratory viruses associated with virus-induced asthma is not fully understood. Therefore, in this article, we review molecular epidemiological studies of RSV, HRV, HPIV, and HMPV infection associated with virus-induced asthma.

  1. Presence and Distribution of Tobacco Viruses in Serbia

    OpenAIRE

    Nataša Duduk; Aleksandra Bulajić; Janoš Berenji; Ivana Đekić; Bojan Duduk; Branka Krstić

    2006-01-01

    Infection with a large number of plant viruses could imperil tobacco yield and quality. Tobacco is a natural host for more than 20 viruses, among which the most important and economically harmful are tobacco mosaic virus (TMV), tomato spotted wilt virus (TSWV), cucumber mosaic virus (CMV), potato virus Y (PVY), alfalfa mosaic virus (AMV), tobacco ringspot virus (TRSV), tobacco each virus (TEV) and tobacco vein mottling virus (TVMV).The occurence and distribution of tobacco viruses were invest...

  2. Aplicación de modelos estructurales marginales para estimar los efectos de la terapia antirretroviral en 5 cohortes de seroconvertores al virus de la inmunodeficiencia humana Marginal structural models application to estimate the effects of antiretroviral therapy in 5 cohorts of HIV seroconverters

    Directory of Open Access Journals (Sweden)

    Santiago Pérez-Hoyos

    2007-02-01

    Full Text Available Los métodos convencionales tienen limitaciones para ajustar por factores de confusión dependientes del tiempo para evaluar la efectividad poblacional de tratamientos en estudios observacionales. En este trabajo se muestra un nuevo tipo de metodología, los modelos estructurales marginales (MEM, y se estima la efectividad de la terapia antirretroviral de gran actividad (TARGA sobre la incidencia de sida o muerte. Sujetos y métodos: Se identificaron los sujetos sin TARGA seguidos a partir de 1997 en las cohortes de seroconvertores al virus de la inmunodeficiencia humana (VIH del proyecto GEMES (Grupo de Estudio Multicéntrico Español de Seroconvertores. Para estimar el efecto sobre la incidencia de sida o muerte, se obtuvieron los parámetros de un MEM mediante una regresión logística ponderada por probabilidad inversa. La estimación de los pesos se basó en el recuento de CD4, el tiempo desde la seroconversión, el sexo, la edad, la categoría de trasmisión y el tratamiento previo. Resultados: Los 917 sujetos elegibles se siguieron durante una media de 3,4 años, durante los cuales se observaron 139 desenlaces de interés. El 42,1% de los participantes recibió TARGA durante el estudio. La tasa relativa fue de 1,01 (intervalo de confianza &(IC& del 95%, 0,68-1,49 mediante un modelo de Cox convencional sin covariables, y de 0,90 (IC del 95%, 0,61-1,32 mediante un modelo de Cox convencional con covariables cambiantes en el tiempo. La tasa relativa causal estimada por un MEM fue de 0,74 (IC del 95%, 0,49-1,12. Conclusiones: El efecto beneficioso del TARGA encontrado por los MEM está bien establecido, pero los modelos convencionales no pudieron detectarlo. El uso de un MEM permitió ajustar apropiadamente por la variable CD4, que es a la vez una variable de confusión dependiente del tiempo y está afectada por el uso previo de tratamiento.Objectives: Standard methods to evaluate population effectiveness of treatments in observational

  3. Altern als Widerstand

    Directory of Open Access Journals (Sweden)

    Roberta Maierhofer

    2005-07-01

    Full Text Available Gullettes kulturwissenschaftliche Untersuchung Aged by Culture ist wie bereits ihre zwei vorangehenden Werke, die sich mit Altern beschäftigen – Safe at Last in the Middle Years: The Invention of the Midlife Progress Novel (1988 und Declining to Decline. Cultural Combat and the Politics of the Middle (1997 –, von großem persönlichen Engagement und durch ein politisches Anliegen motiviert. Sowohl die Dringlichkeit als auch der Widerstand, den Gullette, die sich als „age critic“ definiert, als moralische und politische Notwendigkeit postuliert, werden in der Zweiteilung der Abhandlung angesprochen: „Cultural Urgencies“ und „Theorizing Age Resistantly“. Während Gullette den Begriff „aged by culture“ bereits in Declining to Decline einführt, stellt sie ihn nun in den Mittelpunkt ihrer Untersuchung. Das Buch ist einerseits einer gesellschaftspolitischen Analyse der USA gewidmet, andererseits wird eine Theorie des Widerstands gegenüber Altersdiskriminierung entwickelt.

  4. Pornographie als Metapher

    Directory of Open Access Journals (Sweden)

    Caroline Schubarth

    2010-03-01

    Full Text Available In diesem Artikel werden unterschiedliche metaphorische Verwendungen des Pornografiebegriffs und deren Implikationen untersucht. Während die Existenz von Pornografie der feministischen Anti-Porno-Bewegung als Erklärung für die anhaltende Diskriminierung von Frauen in westlichen Gesellschaften dient, nutzen rechtskonservative Kräfte den Pornografievorwurf als Rechtfertigung für die Zensur von als deviant empfundenen Identitäten und sexuellen Praktiken.This article examines different metaphorical uses of the term pornography and the resulting implications. While the existence of pornography for the feminist anti-porno movement serves as factor in the continuing discrimination of women in western societies, conservative powers on the right use the allegation of pornography as a justification for censorship of those identities and sexual practices deemed deviant.

  5. Single virus genomics: a new tool for virus discovery.

    Directory of Open Access Journals (Sweden)

    Lisa Zeigler Allen

    Full Text Available Whole genome amplification and sequencing of single microbial cells has significantly influenced genomics and microbial ecology by facilitating direct recovery of reference genome data. However, viral genomics continues to suffer due to difficulties related to the isolation and characterization of uncultivated viruses. We report here on a new approach called 'Single Virus Genomics', which enabled the isolation and complete genome sequencing of the first single virus particle. A mixed assemblage comprised of two known viruses; E. coli bacteriophages lambda and T4, were sorted using flow cytometric methods and subsequently immobilized in an agarose matrix. Genome amplification was then achieved in situ via multiple displacement amplification (MDA. The complete lambda phage genome was recovered with an average depth of coverage of approximately 437X. The isolation and genome sequencing of uncultivated viruses using Single Virus Genomics approaches will enable researchers to address questions about viral diversity, evolution, adaptation and ecology that were previously unattainable.

  6. Neuroteratogenic Viruses and Lessons for Zika Virus Models.

    Science.gov (United States)

    Kim, Kenneth; Shresta, Sujan

    2016-08-01

    The Centers for Disease Control and Prevention has confirmed that Zika virus (ZIKV) causes congenital microcephaly. ZIKV now joins five other neuroteratogenic (NT) viruses in humans and ZIKV research is in its infancy. In addition, there is only one other NT human arbovirus (Venezuelan equine encephalitis virus), which is also poorly understood. But further insight into ZIKV can be found by evaluating arboviruses in domestic animals, of which there are at least seven NT viruses, three of which have been well studied. Here we review two key anatomical structures involved in modeling transplacental NT virus transmission: the placenta and the fetal blood-brain barrier. We then survey major research findings regarding transmission of NT viruses for guidance in establishing a mouse model of Zika disease that is crucial for a better understanding of ZIKV transmission and pathogenesis. PMID:27387029

  7. 9 CFR 113.215 - Bovine Virus Diarrhea Vaccine, Killed Virus.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Bovine Virus Diarrhea Vaccine, Killed... REQUIREMENTS Killed Virus Vaccines § 113.215 Bovine Virus Diarrhea Vaccine, Killed Virus. Bovine Virus Diarrhea... paragraph. (i) Eight bovine virus diarrhea susceptible calves (five vaccinates and three controls) shall...

  8. Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR

    OpenAIRE

    Drosten, Christian; Göttig, Stephan; Schilling, Stefan; Asper, Marcel; Panning, Marcus; Schmitz, Herbert; Günther, Stephan

    2002-01-01

    Viral hemorrhagic fevers (VHFs) are acute infections with high case fatality rates. Important VHF agents are Ebola and Marburg viruses (MBGV/EBOV), Lassa virus (LASV), Crimean-Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), dengue virus (DENV), and yellow fever virus (YFV). VHFs are clinically difficult to diagnose and to distinguish; a rapid and reliable laboratory diagnosis is required in suspected cases. We have established six one-step, real-time reverse transcripti...

  9. Wikipedia als Referenzorgan

    OpenAIRE

    Voß, Jakob

    2006-01-01

    Wikipedia wird inzwischen von vielen Nutzern regelmäßig als Nachschlagewerk genutzt. Aufgrund des offenen Charakters der Online-Enzyklopädie, bei der sich jeder direkt beteiligen kann, ist jedoch zusätzliche Medienkompetenz notwendig, um die Qualität einzelner Wikipedia-Artikel einschätzen zu können. Im Vortrag sollen Methoden zur Beurteilung von Wikipedia-Artikeln dargestellt und die Frage beleuchtet werden, unter welchen Umständen Wikipedia als Referenzorgan geeignet ist.

  10. Resveratrol, sirtuins, and viruses.

    Science.gov (United States)

    Yang, Tao; Li, Shugang; Zhang, Xuming; Pang, Xiaowu; Lin, Qinlu; Cao, Jianzhong

    2015-11-01

    Resveratrol is a natural phenolic product found in some plants in response to stress and has been linked to the many health benefits of red wine. Over the past several decades, a great deal of research has identified diverse biological roles associated with resveratrol, including anti-oxidant, anti-proliferation, anti-inflammation, anti-cancer, anti-fungal, and antiviral activities. Such biological activities of resveratrol are likely mediated through multiple cellular targets or pathways, such as sirtuins, a family of NAD(+)-dependent deacetylases. In this treatise, the literatures focusing on the roles of resveratrol and sirtuins in modulating infections by a broad-spectrum of viruses are reviewed, with an emphasis on its potential antiviral mechanisms. A working model about the effects of resveratrol on virus infection is proposed to stimulate further researches on this exciting topic. PMID:26479742

  11. Vade retro virus

    OpenAIRE

    Bardini, Thierry

    2015-01-01

    Avec l’aide de récents résultats de la virologie, le présent article se concentre sur les virus en tant que participants d’une possible redéfinition de la frontière inférieure de la vie, en tant que vie minimale. À l’heure du triomphe de la viralité dans la cyberculture contemporaine, l’auteur avance que l’on devrait considérer les virus comme les premières formes d’entités convergentes, c’est-à-dire existant par-delà la division du monde en deux phases distinctes et incompatibles, numérique ...

  12. [Ebola virus disease: Update].

    Science.gov (United States)

    de la Calle-Prieto, Fernando; Arsuaga-Vicente, Marta; Mora-Rillo, Marta; Arnalich-Fernandez, Francisco; Arribas, Jose Ramon

    2016-01-01

    The first known Ebola outbreak occurred in 1976. Since then, 24 limited outbreaks had been reported in Central Africa, but never affecting more than 425 persons. The current outbreak in Western Africa is the largest in history with 28,220 reported cases and 11,291 deaths. The magnitude of the epidemic has caused worldwide alarm. For the first time, evacuated patients were treated outside Africa, and secondary cases have occurred in Spain and the United States. Since the start of the current epidemic, our knowledge about the epidemiology, clinical picture, laboratory findings, and virology of Ebola virus disease has considerably expanded. For the first time, experimental treatment has been tried, and there have been spectacular advances in vaccine development. A review is presented of these advances in the knowledge of Ebola virus disease. PMID:26774254

  13. Alstroemeria-infecting cucumber mosaic virus isolates contain additional sequences in the RNA 3 segment.

    OpenAIRE

    Chen, Y.K; Prins, M.W.; Derks, A.F.L.M.; Langeveld, S A; Goldbach, R.W.

    2002-01-01

    The coat protein (CP) genes and flanking regions of three alstroemeria-infecting cucumber mosaic virus isolates (CMV-ALS), denoted ALS-LBO, ALS-IPO, and ALS-NAK, were cloned and their nucleotide sequence determined and compared at both nucleic acid and deduced protein level with the published sequences of CMV RNA 3. The sequences of these isolates showed more than 95% nucleotide and peptide sequence homology to each other and to other members of subgroup II CMV. Strikingly, an additional sequ...

  14. Proteorhodopsin genes in giant viruses

    Directory of Open Access Journals (Sweden)

    Yutin Natalya

    2012-10-01

    Full Text Available Abstract Viruses with large genomes encode numerous proteins that do not directly participate in virus biogenesis but rather modify key functional systems of infected cells. We report that a distinct group of giant viruses infecting unicellular eukaryotes that includes Organic Lake Phycodnaviruses and Phaeocystis globosa virus encode predicted proteorhodopsins that have not been previously detected in viruses. Search of metagenomic sequence data shows that putative viral proteorhodopsins are extremely abundant in marine environments. Phylogenetic analysis suggests that giant viruses acquired proteorhodopsins via horizontal gene transfer from proteorhodopsin-encoding protists although the actual donor(s could not be presently identified. The pattern of conservation of the predicted functionally important amino acid residues suggests that viral proteorhodopsin homologs function as sensory rhodopsins. We hypothesize that viral rhodopsins modulate light-dependent signaling, in particular phototaxis, in infected protists. This article was reviewed by Igor B. Zhulin and Laksminarayan M. Iyer. For the full reviews, see the Reviewers’ reports section.

  15. Geographic distribution of Bhanja virus.

    Science.gov (United States)

    Hubálek, Z

    1987-01-01

    A review on the geographic distribution, vectors and hosts of Bhanja virus (Bunyaviridae) is based on reports about: isolations of the virus; antibody surveys. Bhanja virus has been isolated in 15 countries of Asia, Africa and Europe, and antibodies against it have been detected in 15 additional countries. Vector range includes ticks of the family Ixodidae (subfam. Amblyomminae; not subfam. Ixodinae): 13 species of 6 genera (Haemaphysalis, Dermacentor, Hyalomma, Amblyomma, Rhipicephalus and Boophilus) yielded the virus. Bhanja virus has only rarely been isolated from vertebrates (Atelerix, Xerus, Ovis, Bos; possibly bats), though antibodies have been detected frequently in a wide range of mammals (Ruminantia being the major hosts), in several species of birds (Passeriformes, Galliformes) and even reptiles (Ophisaurus apodus). Natural foci of the Bhanja virus infections are of the boskematic type (sensu Rosický), associated closely with pastures of domestic ruminants infested by ticks in the regions of tropical, subtropical and partly temperate climatic zones. PMID:3108117

  16. BERKELEY: ALS ring

    International Nuclear Information System (INIS)

    Everybody at Lawrence Berkeley Laboratory's Center for Beam Physics is pleased with the rapid progress in commissioning LBL's Advanced Light Source (ALS) electron storage ring, the foundation for this third-generation synchrotron radiation facility. Designed for a maximum current of 400 mA, the ALS storage ring reached 407 mA just 24 days after storing the first beam on 16 March. ALS construction as a US Department of Energy (DOE) national user facility to provide high-brightness vacuum ultra-violet and soft x-ray radiation began in October 1987. One technical requirement marking project completion was to accumulate a 50-mA current in the storage ring. The ALS passed this milestone on 24 March, a week ahead of the official deadline. Once injected, the electron beam decays quasi-exponentially primarily because of interactions with residual gas molecules in the storage-ring vacuum chamber. Eventually, when the pressure in the vacuum chamber with beam decreases toward the expected operating level of 1 nano Torr, it will only be necessary to refill the storage ring at intervals of four to eight hours. At present the vacuum is improving rapidly as surfaces are irradiated (scrubbed) by the synchrotron radiation itself. At 100 mA, beam lifetime was about one hour (9 April)

  17. Al Shanker Remembers.

    Science.gov (United States)

    American Educator, 2000

    2000-01-01

    In a 1996 interview shortly before his death, Al Shanker, longtime president of the American Federation of Teachers, discussed such topics as: his own educational experiences; how he learned about political fighting in the Boy Scouts; the appeal of socialism; multinational corporations and the nation state; teaching tough students; and John Dewey…

  18. Ebola Virus Disease

    Centers for Disease Control (CDC) Podcasts

    2014-08-08

    This podcast provides general information about Ebola virus disease and the outbreak in West Africa. The program contains remarks from CDC Director Dr. Tom Frieden, as well as a brief description of CDC’s response efforts.  Created: 8/8/2014 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 8/8/2014.

  19. Framework Ebola Virus Disease

    OpenAIRE

    2014-01-01

    The current outbreak of Ebola virus disease (EVD) in West Africa is the largest recognized outbreak of this disease to date. It is also the first such outbreak including cases infected outside of Africa. The risk of an introduction of EVD to Germany (defined as an infected person entering Germany and passing the infection to others) is very low. But it cannot be totally excluded that in isolated instances infected persons could enter unrecognizably during the incubation period, potentiall...

  20. Viruses and drinking water

    OpenAIRE

    Cartwright, R Y

    1997-01-01

    There is no evidence to indicate that there is a risk of acquiring a virus infection through the consumption of properly treated drinking water, provided the integrity of the distribution system is maintained and there is no post-treatment contamination. The consumption of inadequately treated, untreated or post-treatment contaminated water is, however, associated with a risk of hepatitis A, hepatitis E and viral gastroenteritis. The use of the standard bacterial indicators for water monitori...

  1. Parainfluenza Virus Infection.

    Science.gov (United States)

    Branche, Angela R; Falsey, Ann R

    2016-08-01

    Human parainfluenza viruses (HPIVs) are single-stranded, enveloped RNA viruses of the Paramyoviridaie family. There are four serotypes which cause respiratory illnesses in children and adults. HPIVs bind and replicate in the ciliated epithelial cells of the upper and lower respiratory tract and the extent of the infection correlates with the location involved. Seasonal HPIV epidemics result in a significant burden of disease in children and account for 40% of pediatric hospitalizations for lower respiratory tract illnesses (LRTIs) and 75% of croup cases. Parainfluenza viruses are associated with a wide spectrum of illnesses which include otitis media, pharyngitis, conjunctivitis, croup, tracheobronchitis, and pneumonia. Uncommon respiratory manifestations include apnea, bradycardia, parotitis, and respiratory distress syndrome and rarely disseminated infection. Immunity resulting from disease in childhood is incomplete and reinfection with HPIV accounts for 15% of respiratory illnesses in adults. Severe disease and fatal pneumonia may occur in elderly and immunocompromised adults. HPIV pneumonia in recipients of hematopoietic stem cell transplant (HSCT) is associated with 50% acute mortality and 75% mortality at 6 months. Though sensitive molecular diagnostics are available to rapidly diagnose HPIV infection, effective antiviral therapies are not available. Currently, treatment for HPIV infection is supportive with the exception of croup where the use of corticosteroids has been found to be beneficial. Several novel drugs including DAS181 appear promising in efforts to treat severe disease in immunocompromised patients, and vaccines to decrease the burden of disease in young children are in development. PMID:27486735

  2. Computer viruses and electronic mail

    OpenAIRE

    Buzzi, Marina

    2002-01-01

    Today the Internet is a valuable source of information as well as a powerful communication medium, with undoubted social and economic benefits, however it also poses some security risks. Virsuses may hide in email attachments or in appartently innocent applications directly downloadable from the Internet. In this work we give a brief overview of virus types and main defense techniques. Then we present statistical data of virus attacks revealed by an anti-virus SW activated on our e-mail serve...

  3. Lagos Bat Virus in Kenya▿

    OpenAIRE

    Kuzmin, Ivan V.; Niezgoda, Michael; Franka, Richard; Agwanda, Bernard; Markotter, Wanda; Beagley, Janet C.; Urazova, Olga Y.; Breiman, Robert F.; Rupprecht, Charles E.

    2008-01-01

    During lyssavirus surveillance, 1,221 bats of at least 30 species were collected from 25 locations in Kenya. One isolate of Lagos bat virus (LBV) was obtained from a dead Eidolon helvum fruit bat. The virus was most similar phylogenetically to LBV isolates from Senegal (1985) and from France (imported from Togo or Egypt; 1999), sharing with these viruses 100% nucleoprotein identity and 99.8 to 100% glycoprotein identity. This genome conservancy across space and time suggests that LBV is well ...

  4. Variant (Swine Origin) Influenza Viruses in Humans

    Science.gov (United States)

    ... Past Newsletters Variant (Swine Origin) Influenza Viruses in Humans Language: English Español Recommend on Facebook Tweet ... Page Background Reporting Additional Information Key Facts about Human Infections with Variant Viruses (Swine Origin Influenza Viruses ...

  5. Dengue virus growth, purification, and fluorescent labeling.

    Science.gov (United States)

    Zhang, Summer; Chan, Kuan Rong; Tan, Hwee Cheng; Ooi, Eng Eong

    2014-01-01

    The early events of the dengue virus life cycle involve virus binding, internalization, trafficking, and fusion. Fluorescently labeled viruses can be used to visualize these early processes. As dengue virus has 180 identical copies of the envelope protein attached to the membrane surface and is surrounded by a lipid membrane, amine-reactive (Alexa Fluor) or lipophilic (DiD) dyes can be used for virus labeling. These dyes are highly photostable and are ideal for studies involving cellular uptake and endosomal transport. To improve virus labeling efficiency and minimize the nonspecific labeling of nonviral proteins, virus concentration and purification precede fluorescent labeling of dengue viruses. Besides using these viruses for single-particle tracking, DiD-labeled viruses can also be used to distinguish serotype-specific from cross-neutralizing antibodies. Here the details of virus concentration, purification, virus labeling, applications, and hints of troubleshooting are described. PMID:24696327

  6. Zika Virus and Complications: Questions and Answers

    Science.gov (United States)

    ... Feed Youtube Twitter Facebook Google + iTunes Play Store Zika virus and complications: Questions and answers Online Q& ... a cause of microcephaly and Guillain-Barré syndrome. Zika virus Updated! How do people catch Zika virus? ...

  7. Chikungunya Virus: What You Need to Know

    Science.gov (United States)

    Chikungunya Virus: What you need to know Chikungunya (pronunciation: \\chik-en-gun-ye) is: A virus spread through Aedes species mosquito bites. Aedes mosquitoes also spread dengue and Zika viruses. A risk to anyone traveling to a region ...

  8. Linfomas en pacientes con infección por el virus de la inmunodeficiencia humana. Influencia de la terapia antirretroviral de gran actividad en las características clinico-biológicas, la respuesta al tratamiento y la supervivencia

    OpenAIRE

    Navarro Ferrando, José Tomás

    2004-01-01

    Introducción. Los linfomas son la segunda neoplasia en frecuencia, que afecta a los pacientes infectados por el virus de la inmunodeficiencia humana (VIH). Desde la aparición del tratamiento antirretroviral de gran actividad (TARGA) el pronóstico de los pacientes infectados por el VIH ha cambiado, y se ha observado una disminución en la incidencia de infecciones oportunistas y algunas neoplasias. El uso generalizado del TARGA ha determinado que los factores pronósticos de los pacientes con li...

  9. Genome of crocodilepox virus.

    Science.gov (United States)

    Afonso, C L; Tulman, E R; Delhon, G; Lu, Z; Viljoen, G J; Wallace, D B; Kutish, G F; Rock, D L

    2006-05-01

    Here, we present the genome sequence, with analysis, of a poxvirus infecting Nile crocodiles (Crocodylus niloticus) (crocodilepox virus; CRV). The genome is 190,054 bp (62% G+C) and predicted to contain 173 genes encoding proteins of 53 to 1,941 amino acids. The central genomic region contains genes conserved and generally colinear with those of other chordopoxviruses (ChPVs). CRV is distinct, as the terminal 33-kbp (left) and 13-kbp (right) genomic regions are largely CRV specific, containing 48 unique genes which lack similarity to other poxvirus genes. Notably, CRV also contains 14 unique genes which disrupt ChPV gene colinearity within the central genomic region, including 7 genes encoding GyrB-like ATPase domains similar to those in cellular type IIA DNA topoisomerases, suggestive of novel ATP-dependent functions. The presence of 10 CRV proteins with similarity to components of cellular multisubunit E3 ubiquitin-protein ligase complexes, including 9 proteins containing F-box motifs and F-box-associated regions and a homologue of cellular anaphase-promoting complex subunit 11 (Apc11), suggests that modification of host ubiquitination pathways may be significant for CRV-host cell interaction. CRV encodes a novel complement of proteins potentially involved in DNA replication, including a NAD(+)-dependent DNA ligase and a protein with similarity to both vaccinia virus F16L and prokaryotic serine site-specific resolvase-invertases. CRV lacks genes encoding proteins for nucleotide metabolism. CRV shares notable genomic similarities with molluscum contagiosum virus, including genes found only in these two viruses. Phylogenetic analysis indicates that CRV is quite distinct from other ChPVs, representing a new genus within the subfamily Chordopoxvirinae, and it lacks recognizable homologues of most ChPV genes involved in virulence and host range, including those involving interferon response, intracellular signaling, and host immune response modulation. These data

  10. Towards Metamorphic Virus Recognition Using Eigenviruses

    OpenAIRE

    Saleh, Moustafa

    2012-01-01

    Metamorphic viruses are considered the most dangerous of all computer viruses. Unlike other computer viruses that can be detected statically using static signature technique or dynamically using emulators, metamorphic viruses change their code to avoid such detection techniques. This makes metamorphic viruses a real challenge for computer security researchers. In this thesis, we investigate the techniques used by metamorphic viruses to alter their code, such as trivial code insertion, instruc...

  11. Epigenetic mechanisms in virus-induced tumorigenesis

    OpenAIRE

    Poreba, Elzbieta; Broniarczyk, Justyna Karolina; Gozdzicka-Jozefiak, Anna

    2011-01-01

    About 15–20% of human cancers worldwide have viral etiology. Emerging data clearly indicate that several human DNA and RNA viruses, such as human papillomavirus, Epstein–Barr virus, Kaposi’s sarcoma-associated herpesvirus, hepatitis B virus, hepatitis C virus, and human T-cell lymphotropic virus, contribute to cancer development. Human tumor-associated viruses have evolved multiple molecular mechanisms to disrupt specific cellular pathways to facilitate aberrant replication. Although oncogeni...

  12. Molecular Basis for the Selective Inhibition of Respiratory Syncytial Virus RNA Polymerase by 2'-Fluoro-4'-Chloromethyl-Cytidine Triphosphate.

    Directory of Open Access Journals (Sweden)

    Jerome Deval

    2015-06-01

    Full Text Available Respiratory syncytial virus (RSV causes severe lower respiratory tract infections, yet no vaccines or effective therapeutics are available. ALS-8176 is a first-in-class nucleoside analog prodrug effective in RSV-infected adult volunteers, and currently under evaluation in hospitalized infants. Here, we report the mechanism of inhibition and selectivity of ALS-8176 and its parent ALS-8112. ALS-8176 inhibited RSV replication in non-human primates, while ALS-8112 inhibited all strains of RSV in vitro and was specific for paramyxoviruses and rhabdoviruses. The antiviral effect of ALS-8112 was mediated by the intracellular formation of its 5'-triphosphate metabolite (ALS-8112-TP inhibiting the viral RNA polymerase. ALS-8112 selected for resistance-associated mutations within the region of the L gene of RSV encoding the RNA polymerase. In biochemical assays, ALS-8112-TP was efficiently recognized by the recombinant RSV polymerase complex, causing chain termination of RNA synthesis. ALS-8112-TP did not inhibit polymerases from host or viruses unrelated to RSV such as hepatitis C virus (HCV, whereas structurally related molecules displayed dual RSV/HCV inhibition. The combination of molecular modeling and enzymatic analysis showed that both the 2'F and the 4'ClCH2 groups contributed to the selectivity of ALS-8112-TP. The lack of antiviral effect of ALS-8112-TP against HCV polymerase was caused by Asn291 that is well-conserved within positive-strand RNA viruses. This represents the first comparative study employing recombinant RSV and HCV polymerases to define the selectivity of clinically relevant nucleotide analogs. Understanding nucleotide selectivity towards distant viral RNA polymerases could not only be used to repurpose existing drugs against new viral infections, but also to design novel molecules.

  13. Computer virus information update CIAC-2301

    Energy Technology Data Exchange (ETDEWEB)

    Orvis, W.J.

    1994-01-15

    While CIAC periodically issues bulletins about specific computer viruses, these bulletins do not cover all the computer viruses that affect desktop computers. The purpose of this document is to identify most of the known viruses for the MS-DOS and Macintosh platforms and give an overview of the effects of each virus. The authors also include information on some windows, Atari, and Amiga viruses. This document is revised periodically as new virus information becomes available. This document replaces all earlier versions of the CIAC Computer virus Information Update. The date on the front cover indicates date on which the information in this document was extracted from CIAC`s Virus database.

  14. ALS insertion devices

    International Nuclear Information System (INIS)

    The Advanced Light Source (ALS), the first US third generation synchrotron radiation source, is currently under construction at the Lawrence Berkeley Laboratory. The low-emittance, 1.5 GeV electron storage ring and the insertion devices are specifically designed to produce high brightness beams in the UV to soft X-Ray range. The planned initial complement of insertion devices includes four 4.6 m long undulators, with period lengths of 3.9 cm, 5.0 cm (2) and 8.0 cm, and a 2.9 m long wiggler of 16 cm period length. Undulator design is well advanced and fabrication has begun on the 5.0 cm and 8.0 cm period length undulators. This paper discusses ALS insertion device requirements; general design philosophy; and design of the magnetic structure, support structure/drive systems, control system and vacuum system. 18 refs., 9 figs., 5 tabs

  15. De GPS al mapa

    Directory of Open Access Journals (Sweden)

    Esteban Dörries

    2016-03-01

    Full Text Available Las coordenadas Lambert obtenidas a partir de mediciones con equipos GPS de mano, llamados a veces navegadores, en ciertos casos confunden al usuario, por diferir claramente de su posición real al ser graficadas en un mapa del Instituto Geográfico Nacional (IGN: Esto puede resolverse con suficiente exactitud mediante una transformación de Molodensky, seguida de la correspondiente proyección cartográfica. Sin embargo, los tres parámetros necesarios para la transformación, supuestamente válidos para Costa Rica, se encuentran en muchas variantes y producen obviamente resultados diferentes. En este trabajo se analizan los fundamentos del problema y sus posibles soluciones, culminando con un estudio comparativo de ocho casos, que permite seleccionar los valores más adecuados para los parámetros.

  16. Three-dimensional structure of the inner core of rice dwarf virus

    Institute of Scientific and Technical Information of China (English)

    SHAO; Chenghua

    2001-01-01

    [1]Suzuki, N., Sugawara, M., Kusano, T. et al., Immunodetection of rice dwarf phytoreoviral protein in both insect and plant hosts, Virology, 1994, 202: 41.[2]Omura, T., Ishikawa, K., Hirano, H. et al., The outer capid protein of rice dwarf virus is encoded by genome segment S8, J. Gen. Virol., 1989, 70: 2759.[3]Lu, G. Y., Zhou, Z. H., Baker, M. L. et al., Structure of double-shelled rice dwarf virus, J.Virol., 1998, 72: 8541.[4]Reinisch, K. M., Nibert, M. L., Harrison, S. C., Structure of the reovirus core at 3.6 ? resolution, Nature, 2000, 404: 960.[5]Zhang, H., Zhang, J., Yu, X. et al., Visualization of protein-RNA interactions in cytoplasmic polyhedrosis virus, J. Virol., 1999, 73: 1624.[6]Zhou, Z. H., Hardt, S., Wang, B. et al., CTF determination of images of ice-embedded single particles using a graphics inter-face, J. Struct. Biol., 1996, 116: 216.[7]Zhou, Z. H., Chiu, W., Haskell, K. et al., Refinement of herpesvirus B-capsid using parallel supercomputers, Biophys. J., 1998, 74: 576.[8]Zhou, Z. H., He, J., Jakana, J. et al., Assembly of VP26 in HSV-1 inferred from structures of wild-type and recombinant cap-sids, Nature Struct. Biol., 1995, 2: 1026.[9]Grimes, J. M., Burroughs, J. N., Patrice, G. et al., The atomic structure of the bluetongue virus core, Nature, 1998, 395: 470.[10] Lawton, J. A., Estes, M. K., Prasad, B. V. V., Three-dimensional visualization of mRNA release from actively transcribing rotavirus particles, Nat. Struc. Bio., 1997, 4: 118.[11] Ueda, S., Masuta, C., Uyeda, I., Hypothesis on particle structure and assembly of rice dwarf phytoreovirus: interactions among multiple structural proteins, J.Gen.Virol., 1997, 78: 3135.[12] Kano, H., Koizumi, M., Noda, H. et al., Nucleotide sequence of rice dwarf virus(RDV) genome segment S3 coding for 114 K major core protein, Nucleic Acids Res., 1990, 18: 6700.[13] Nakata, M., Fukunaga, K., Suzuki, N., Polypeptide components of rice dwarf virus, Ann

  17. Los virus Influenza y la nueva pandemia A/H1N1

    Directory of Open Access Journals (Sweden)

    Miguel Talledo

    2011-07-01

    Full Text Available Los virus Influenza pertenecen a la familia Orthomyxoviridae, virus con genoma RNA de sentido negativo segmentado. Los virus influenza tipo A infectan a humanos y otros organismos, y son los agentes causantes de influenza en humanos. Resaltan entre sus principales proteínas la Hemaglutinina y la Neuraminidasa, que son utilizadas en la clasificación de los miembros de este grupo. Estos virus mutan continuamente, exhibiendo patrones muy estudiados, como el cambio y la deriva antigénica, siendo uno de los principales eventos de recombinación el reordenamiento. Todos los subtipos se encuentran en aves acuáticas silvestres, aunque se han encontrado otros hospederos, como equinos, visones, ballenas, focas, cerdos, gallinas y pavos, entre otros. Tanto las aves salvajes, las aves domésticas y el cerdo juegan un rol fundamental en la adaptación progresiva del virus al hospedero humano. Aunque los subtipos H2N2 y H3N2 han sido muy comunes, el subtipo H1N1 ha reemergido con mutaciones que le han permitido alcanzar el estado de pandemia en 2009. Este nuevo virus surge de un virus generado por triple reordenamiento con el virus humano, porcino norteamericano y aviar, conteniendo a su vez segmentos génicos de virus influenza porcina euroasiática. Esto ha hecho que el virus presente una enfermedad humana moderada y solamente severa y hasta letal en casos de individuos con condiciones médicas previas. A nivel mundial ha causado más de 134,510 casos y en el Perú alcanza cerca de 3,700 casos. El estado actual indica que la pandemia está por llegar a su pico máximo en el Perú, debido a la alta morbilidad del virus coincidente con la estación más fría del año. Es importante contener al máximo la dispersión del virus, ya que cuanto mayor sea el número de personas que infecte, el mismo estará sometido a un mayor número de eventos de recombinación genética por reordenamiento con virus influenza humanos previos y esto puede condicionar a la

  18. Acarbose als Carbophor

    OpenAIRE

    Merettig, Nadine

    2014-01-01

    Das Pseudotetrasaccharid Acarbose ist seit 1990 als kompetitiver Inhibitor intestinaler α-Glucosidasen (GlucobayTM/PrecoseTM) in der medizinischen Anwendung bei der Therapie von Diabetes mellitus Typ-2. Charakteristisch für die Struktur der Acarbose ist die Acarviosyleinheit, bei der Valinamin und Desoxyglucose N-glycosidisch verknüpft sind; des Weiteren ist an die Acarviosyleinheit ein Maltosylrest gebunden. Der Inhibitoreffekt beruht auf der irreversiblen Bindung zwischen ...

  19. Augmented Reality als Bildungsenhancement?

    OpenAIRE

    Damberger, Thomas

    2015-01-01

    Die Realität, die sich mithilfe von Datenbrillen und Smartphone Applikationen in die virtuelle Welt hinein ausdehnt, erfährt eine Form des Enhancements. Ein solches Enhancement kann unter bestimmten Voraussetzungen als Bildungsenhancement verstanden werden. Der Text befasst sich mit Formen der erweiterten Realität, ferner mit dem, um was es wesentlich bei der Bildung geht und zuletzt mit den Bedingungen, die erforderlich sind, um diesem Wesentlichen mit Hilfe von augmented reality besser zum ...

  20. Carta al editor

    OpenAIRE

    Harry Pachajoa

    2012-01-01

    Recently, Valencia et al., published a very interesting article called «Lipid profile in a group of patients with Turner’s syndrome at Clínica Universitaria Bolivariana in the city of Medellín between 2000 and 2009". An article which evaluated lipid levels in patients withTurner syndrome in a Colombian clinic, the study associated results of lipid profile with karyotype and other risk factors for coronary heart disease.

  1. Evolution of Computer Virus Concealment and Anti-Virus Techniques: A Short Survey

    OpenAIRE

    Rad, Babak Bashari; Masrom, Maslin; Ibrahim, Suhaimi

    2011-01-01

    This paper presents a general overview on evolution of concealment methods in computer viruses and defensive techniques employed by anti-virus products. In order to stay far from the anti-virus scanners, computer viruses gradually improve their codes to make them invisible. On the other hand, anti-virus technologies continually follow the virus tricks and methodologies to overcome their threats. In this process, anti-virus experts design and develop new methodologies to make them stronger, mo...

  2. Characterization of the viral proteins involved in the RNA replication of cowpea mosaic virus.

    OpenAIRE

    Bokhoven, van, LJA Laurens

    1993-01-01

    Veel virussen die planten of dieren infecteren hebben enkelstrengs RNA moleculen met een positieve polariteit als drager van hun genetische informatie. Genomische RNA moleculen met positieve polariteit, of (+)-strengs RNA, betekent dat deze RNA moleculen in de cel van de gastheer gebruikt worden als boodschapper RNA voor de productie van virale eiwitten. Deze eiwitten maken dan de verschillende stappen in de levenscyclus van het virus mogelijk, zoals de vermenigvuldiging van het virale RNA (r...

  3. Funktionelle und genetische Analyse des Latenten Membranproteins 1 des Epstein-Barr Virus

    OpenAIRE

    Dirmeier, Ulrike

    2002-01-01

    Epstein-Barr Virus (EBV) infiziert ruhende primäre humane B-Zellen und induziert deren unbegrenzte Proliferation. Dieser Prozess der B-Zell-Immortalisation ist ein Modellsystem, das die pathogenetischen Mechanismen bei der Tumorentstehung widerspiegelt. In vitro gilt das virale latente Membranprotein 1 (LMP1) für die Immortalisation von B-Zellen als essentiell. LMP1 ist ein integrales Membranprotein, das als konstitutiv aktivierter Pseudorezeptor verschiedene Signalwege in der ...

  4. Reverse genetics with animal viruses

    International Nuclear Information System (INIS)

    Full text: Reverse genetics of negative-strand RNA viruses (NSV), which allows generation of recombinant viruses entirely from cloned cDNA, has progressed rapidly in the past decade. NSV are a large and diverse group of enveloped viruses of both medical and veterinary importance. They differ widely in morphology, genome structure and host interactions. The first NSV that was completely amenable to genetic manipulation is the neurotropathogenic rabies virus of the rhabdovirus family. In subsequent years, vesicular stomatitis virus and a number of viruses belonging to the family Paramyxoviridae, including viruses causing important animal diseases such as rinderpest virus, canine distemper virus, bovine respiratory syncytial virus, bovine parainfluenza virus and Newcastle disease virus (NDV), succumbed to genetic engineering. The ability to genetically manipulate NSV opens a wide range of possibilities to study the virus biology and develop improved vaccines. Identification and analysis of attenuating mutations using the recombinant system could lead to generation of safe vaccine strains. Introduction of one of the previously studied mutation into an infectious rabies virus (RV) clone by replacing the arginine at position 333 of RV glycoprotein (G-protein) by an aspartic acid resulted in a dramatic attenuation. Combination of this mutation with a deletion that eliminates the interaction between RV P-protein and the cytoplasmic dynein light chain (LC8), which is presumably involved in retrograde transport of RV, further attenuates the rabies virus by 30-fold after intramuscular inoculation. Since extreme attenuation may adversely affect immunogenicity, reverse genetics was used to introduce an additional Gprotein to the step-wise attenuated RV to increase its effectiveness. The resultant recombinant virus may be helpful in developing a highly safe and effective live RV vaccine for oral immunizations of animals. Reverse genetics of NSV has also helped in providing

  5. Chikungunya: un virus que nos acecha

    Directory of Open Access Journals (Sweden)

    Eugenia Corrales-Aguilar

    2015-03-01

    Full Text Available El virus chikungunya representa una amenaza para Costa Rica. Este arbovirus ha sido introducido al continente americano desde finales de 2013. Debido a sus características epidemiológicas y virológicas, y a la presencia de sus vectores en el país, este virus puede llegar a convertirse en la nueva enfermedad endémica de Costa Rica. Aunque el chikungunya tiene una baja tasa de mortalidad, su alta tasa de ataque podría colapsar el sistema de salud nacional durante una epidemia. En esta revisión se resume aspectos clínicos, virológicos, epidemiológicos y entomológicos relacionados con esta virosis, para identificar, diagnosticar y diferenciar posibles casos de fiebre por chikungunya en el país. Además, se enfatiza en el control epidemiológico y de vectores, para prevenir epidemias de esta enfermedad en Costa Rica.

  6. Field Investigation on the Prevalence of Avian Influenza Virus Infection in Some Localities in Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Abdullah N. Alkhalaf

    2010-07-01

    Full Text Available The objective of this study was to find out prevalence and types of avian influenza virus (AIV among broilers, native chickens, ducks and pigeons in Saudi Arabia. Field investigation was carried out in four localities including Al-Qassim, Hail, Al-Jouf and Northern Border regions. Serum sample, tracheal and cloacal swabs were collected from broilers (n=1561, layers (n=988, ducks (n=329 and pigeons (n=450 from these localities and tested for three different avian influenza viruses (H9, H5 and H3 using Enzyme linked immunosorbent (ELISA test, hamagglutination inhibition (HI test and polymerase chain reaction (PCR. All tested samples were negative for H5 and H3 viruses. In contrast, all positive results were found to be for H9 AI virus using PCR, ELISA and HI test. Chicken sera tested by ELISA for AIV revealed the highest positive samples in Northern Border regions (45.71%, followed by Al-Jouf (29.65%, Al-Qassim (23.98% and Hial (20.94% with non-significant difference (χ2=5.983; P=0.112. HI test carried out on duck sera revealed 35.90% prevalence of antibodies against AIV. PCR amplification resulted in 34.28 and 21.36% positive samples in ducks and chickens, respectively. The highest (45.71% PCR positive chicken samples were from Northern Border regions, followed by Al-Jouf (24.13%, Al-Qassim (19.30% and Hail (16.69% with significant difference (χ2=7.620; P=0.055. All tested pigeons samples were negative for the three virus serotypes included in the study.

  7. Zika Virus Infection and Microcephaly

    Directory of Open Access Journals (Sweden)

    J. Gordon Millichap

    2016-03-01

    Full Text Available A Task Force established by the Brazil Ministry of Health investigated the possible association of microcephaly with Zika virus infection during pregnancy and a registry for microcephaly cases among women suspected to have had Zika virus infection during pregnancy.

  8. Are viruses our oldest ancestors?

    OpenAIRE

    Moelling, Karin

    2012-01-01

    We tend to regard viruses only as pathogens and thereby dismiss their crucial importance for the evolution of life. Viruses were not only the probable precursors of the first cells, but they have helped to shape and build the genomes of all species, including humans.

  9. Zika Virus Infection and Microcephaly.

    Science.gov (United States)

    Millichap, J Gordon

    2016-01-01

    A Task Force established by the Brazil Ministry of Health investigated the possible association of microcephaly with Zika virus infection during pregnancy and a registry for microcephaly cases among women suspected to have had Zika virus infection during pregnancy. PMID:27004142

  10. Genome of Crocodilepox Virus

    OpenAIRE

    Afonso, C. L.; Tulman, E. R.; Delhon, G.; Lu, Z.; Viljoen, G. J.; Wallace, D. B.; Kutish, G. F.; Rock, D. L.

    2006-01-01

    Here, we present the genome sequence, with analysis, of a poxvirus infecting Nile crocodiles (Crocodylus niloticus) (crocodilepox virus; CRV). The genome is 190,054 bp (62% G+C) and predicted to contain 173 genes encoding proteins of 53 to 1,941 amino acids. The central genomic region contains genes conserved and generally colinear with those of other chordopoxviruses (ChPVs). CRV is distinct, as the terminal 33-kbp (left) and 13-kbp (right) genomic regions are largely CRV specific, containin...

  11. Tenosinovitis por virus Chikungunya

    OpenAIRE

    Alfredo Seijo; Victoria Luppo; Alejandra Morales; Elisa Gancedo; Yamila Romer; Jorge Correa; Gladys Poustis; Sergio Giamperetti; Cintia Fabbri; Delia Enría

    2014-01-01

    Se presenta a la consulta un hombre proveniente de la República Dominicana con una tenosinovitis del extensor del dedo medio derecho; en la convalecencia inmediata, segunda curva febril luego de 48 horas de permanecer asintomático de una enfermedad febril aguda, y marcada astenia, exantema pruriginoso, poliartralgias con impotencia funcional y rigidez articular generalizada. Los exámenes bioquímicos no aportaron datos de interés para el diagnóstico. La serología para virus dengue fue negativa...

  12. Feline immunodeficiency virus infection.

    Science.gov (United States)

    Pedersen, N C; Yamamoto, J K; Ishida, T; Hansen, H

    1989-05-01

    Feline immunodeficiency virus (FIV) (formerly feline T-lymphotropic lentivirus or FTLV) was first isolated from a group of cats in Petaluma, California in 1986. The virus is a typical lentivirus in gross and structural morphology. It replicates preferentially but not exclusively in feline T-lymphoblastoid cells, where it causes a characteristic cytopathic effect. The major structural proteins are 10, 17 (small gag), 28 (major core), 31 (endonuclease?), 41 (transmembrane?), 52 (core precursor polyprotein), 54/62 (reverse transcriptase?), and 110/130 (major envelope) kilodaltons in size. The various proteins are antigenically distinguishable from those of other lentiviruses, although serum from EIAV-infected horses will cross-react with some FIV antigens. Kittens experimentally infected with FIV manifest a transient (several days to 2 weeks) fever and neutropenia beginning 4 to 8 weeks after inoculation. This is associated with a generalized lymphadenopathy that persists for up to 9 months. Most cats recover from this initial phase of the disease and become lifelong carriers of the virus. Complete recovery does not occur to any extent in nature or in the laboratory setting. One experimentally infected cat died from a myeloproliferative disorder several months after infection. The terminal AIDS-like phase of the illness has been seen mainly in naturally infected cats. It appears a year or more following the initial infection in an unknown proportion of infected animals. FIV has been identified in cats from all parts of the world. It is most prevalent in high density populations of free roaming cats (feral and pet), and is very uncommon in closed purebred catteries. Male cats are twice as likely to become infected as females. Older male cats adopted as feral or stray animals are at the highest risk of infection, therefore. The infection rate among freely roaming cats rises throughout life, and reaches levels ranging from less than 1% to 12% or more depending on the

  13. Sunshine virus in Australian pythons.

    Science.gov (United States)

    Hyndman, Timothy H; Shilton, Cathy M; Doneley, Robert J T; Nicholls, Philip K

    2012-12-28

    Sunshine virus is a recently discovered novel paramyxovirus that is associated with illness in snakes. It does not phylogenetically cluster within either of the two currently accepted paramyxoviral subfamilies. It is therefore only distantly related to the only other known genus of reptilian paramyxoviruses, Ferlavirus, which clusters within the Paramyxovirinae subfamily. Clinical and diagnostic aspects associated with Sunshine virus are as yet undescribed. The objective of this paper was to report the clinical presentation, virus isolation, PCR testing and pathology associated with Sunshine virus infection. Clinical records and samples from naturally occurring cases were obtained from two captive snake collections and the archives of a veterinary diagnostic laboratory. The clinical signs that are associated with Sunshine virus infection are localised to the neurorespiratory systems or are non-specific (e.g. lethargy, inappetence). Out of 15 snakes that were infected with Sunshine virus (detected in any organ by either virus isolation or PCR), the virus was isolated from four out of ten (4/10) sampled brains, 3/10 sampled lungs and 2/7 pooled samples of kidney and liver. In these same 15 snakes, PCR was able to successfully detect Sunshine virus in fresh-frozen brain (11/11), kidney (7/8), lung (8/11) and liver (5/8); and various formalin-fixed paraffin-embedded tissues (7/8). During a natural outbreak of Sunshine virus in a collection of 32 snakes, the virus could be detected in five out of 39 combined oral-cloacal swabs that were collected from 23 of these snakes over a 105 day period. All snakes that were infected with Sunshine virus were negative for reovirus and ferlavirus by PCR. Snakes infected with Sunshine virus reliably exhibited hindbrain white matter spongiosis and gliosis with extension to the surrounding grey matter and neuronal necrosis evident in severe cases. Five out of eight infected snakes also exhibited mild bronchointerstitial pneumonia

  14. Crenarchaeal Viruses: Morphotypes and Genomes,

    DEFF Research Database (Denmark)

    Prangishvili, P.; Basta, P.; Garrett, Roger Antony

    2008-01-01

    not been observed among viruses from the other two domains of life, the Bacteria and the Eukarya. Also the sequences of circular and linear genomes of crenarchaeal viruses are remarkable because the vast majority of predicted genes have no homologs in the public sequence databases. Viruses of the...... demonstrated a simple transcriptional pattern with minimal temporal control. The replication of viral genomes has not been studied experimentally. Nevertheless, some plausible predictions about possible replication strategies could be made based on specific features of several viral genomes. The comparative...... genomics studies revealed that crenarchaeal viruses form a distinctive group, unrelated to any other viruses, with a small pool of shared genes and a unique origin, or more likely, multiple origins....

  15. Giant viruses come of age.

    Science.gov (United States)

    Fischer, Matthias G

    2016-06-01

    Viruses with genomes up to a few megabases in length are a common occurrence in nature, even though they have escaped our notice until recently. These giant viruses infect mainly single-celled eukaryotes and isolation efforts concentrating on amoebal hosts alone have spawned hundreds of viral isolates, featuring viruses with previously unseen virion morphologies and the largest known viral genomes and particles. One of the challenges that lie ahead is to analyze and categorize the available data and to establish an approved classification system that reflects the evolutionary relationships and biological properties of these viruses. Extensive sampling of Acanthamoeba-infecting mimiviruses and initial characterization of their virophage parasites have provided a first blueprint of the genetic diversity and composition of a giant virus clade that will facilitate the taxonomic grouping of these fascinating microorganisms. PMID:26999382

  16. Effects of mutated replicase and movement protein genes on attenuation of tobacco mosaic virus

    Institute of Scientific and Technical Information of China (English)

    YANG; Gong; (

    2001-01-01

    [1]Banerjee, N., Wang, J. Y., Zaitlin, M., A single nucleotide change in the coat protein gene of tobacco mosaic virus is involved in the induction of severe chlorosis, Virology, 1995, 207: 234-239.[2]Dawson, W. O., Bubrick, P., Grantham, G. L., Modifications of the tobacco mosaic virus coat protein gene affecting replication, movement, and symptomatology, Mol. Plant Pathol., 1988, 78: 783-789.[3]Lu, B., Stubbs, G., Culver, J. N., Coat protein interactions involved in tobacco mosaic tobamovirus cross-protection, Virology, 1998, 248: 188-198.[4]Bao, Y. M., Carter, S. A., Nelson,R. S., The 126- and 183-kilodalton proteins of tobacco mosaic virus, and not their common nucleotide sequence, control mosaic symptom formation in tobacco, J. Virol., 1996, 70: 6378-6383.[5]Holt, C. A., Hodgson, A. J., Coker, F. A. et al., Characterization of the masked strain of tobacco mosaic virus: identification of the region responsible for symptom attenuation by analysis of an infectious cDNA clone, Mol. Plant-Microbe Interact., 1990, 3: 417-423.[6]Nishiguchi, M., Kikuchi, S., Kiho, Y. et al., Molecular basis of plant viral virulence, the complete nucleotide sequence of an attenuated strain of tobacco mosaic virus, Nucleic Acids Res., 1985, 13: 5585-5590.[7]Watanabe, Y., Morita, N., Nishiguchi, M.et al., Attenuated strains of tobacco mosaic virus reduced synthesis of a viral protein with a cell to cell movement function, J. Mol. Biol., 1987, 194: 699-704.[8]Lewandowski, D. J., Dawson, W. O., A single amino acid change in tobacco mosaic virus replicase prevents symptom production, Mol. Plant-Microbe Interact., 1993, 6: 157-160.[9]Yang, G., Qiu, B. S., Cloning and infectivity analysis of the cDNAs of tobacco mosaic virus (tomato strain) and its attenuated virus (N14) genomes, Chinese Journal of Biotechnology (in Chinese), 2000, 16: 207-210.[10]Yang, G., Liu, X. G., Qiu, B. S., Complete nucleotid sequences and genome structures of two Chinese tobacco

  17. Original Article: Peroxisome proliferator‐activated receptor and AMP‐activated protein kinase agonists protect against lethal influenza virus challenge in mice

    OpenAIRE

    Moseley, Carson E.; Robert G. Webster; Aldridge, Jerry R.

    2010-01-01

    Please cite this paper as: Moseley et al. (2010) Peroxisome proliferator‐activated receptor and AMP‐activated protein kinase agonists protect against lethal influenza virus challenge in mice. Influenza and Other Respiratory Viruses 4(5), 307–311. Background  A novel influenza A (H1N1) virus was isolated from humans in North America and has developed into the first pandemic of the 21st century. Reports of a global shortage of antiviral drugs, the evolution of drug‐resistant influenza virus var...

  18. Onkolytische Virotherapie : Virus-vermittelte Expression von MCP-1 oder ß-Galaktosidase in Vaccinia-Virus-kolonisierten Tumoren führt zu einer erhöhten Tumorregression

    OpenAIRE

    Seubert, Carolin

    2011-01-01

    Ungeachtet der enormen Entwicklung in Krebsdiagnostik und -Therapie in den letzten Jahren, sind vollständige Heilungsaussichten weiterhin gering und die aktuellen Behandlungsmethoden oftmals mit schwerwiegenden Nebeneffekten verbunden. Aufgrund dessen sind alternative Behandlungsmethoden unbedingt erforderlich und führten zu einer zunehmenden Bedeutung des Vaccinia-Virus als onkolytisches Virus in der Krebstherapie. In der vorliegenden Arbeit wurden zwei mögliche Therapieansätze zur Verstärku...

  19. First report of southern Tomato virus in tomato in the Canary Islands, Spain

    NARCIS (Netherlands)

    Verbeek, M.; Dullemans, A.M.; Espino, A.; Botella, M.; Alfaro-Fernández, A.; Font, M.I.

    2015-01-01

    In October 2006, tomato plants with torrado disease were sampled in Spain. In a sample of cv. Mariana, originating from Gran Canaria, Tomato torrado virus (ToTV, genus Torradovirus) was detected (isolate GCN06; Alfaro-Fernández et al., 2010). In 2013, the sample was further analysed using next-gener

  20. CELL CULTURE STUDIES WITH THE IMC-HZ-1 NONOCCLUDED VIRUS

    Science.gov (United States)

    Studies were conducted on an adventitious agent (Hz-lv) isolated from the IMC-Hz-1 cell line. It appeared identical to the virus first obtained by Granados et al. from a persistent infection of this cell line. Restriction endonuclease digestion of Hz-lv DNA indicated the agent wa...

  1. Advances in alfalfa mosaic virus-mediated expression of anthrax antigen in planta

    International Nuclear Information System (INIS)

    Plant viruses show great potential for production of pharmaceuticals in plants. Such viruses can harbor a small antigenic peptide(s) as a part of their coat proteins (CP) and elicit an antigen-specific immune response. Here, we report the high yield and consistency in production of recombinant alfalfa mosaic virus (AlMV) particles for specific presentation of the small loop 15 amino acid epitope from domain-4 of the Bacillus anthracis protective antigen (PA-D4s). The epitope was inserted immediately after the first 25 N-terminal amino acids of AlMV CP to retain genome activation and binding of CP to viral RNAs. Recombinant AlMV particles were efficiently produced in tobacco, easily purified for immunological analysis, and exhibited extended stability and systemic proliferation in planta. Intraperitional injections of mice with recombinant plant virus particles harboring the PA-D4s epitope elicited a distinct immune response. Western blotting and ELISA analysis showed that sera from immunized mice recognized both native PA antigen and the AlMV CP

  2. Advances in alfalfa mosaic virus-mediated expression of anthrax antigen in planta.

    Science.gov (United States)

    Brodzik, R; Bandurska, K; Deka, D; Golovkin, M; Koprowski, H

    2005-12-16

    Plant viruses show great potential for production of pharmaceuticals in plants. Such viruses can harbor a small antigenic peptide(s) as a part of their coat proteins (CP) and elicit an antigen-specific immune response. Here, we report the high yield and consistency in production of recombinant alfalfa mosaic virus (AlMV) particles for specific presentation of the small loop 15 amino acid epitope from domain-4 of the Bacillus anthracis protective antigen (PA-D4s). The epitope was inserted immediately after the first 25 N-terminal amino acids of AlMV CP to retain genome activation and binding of CP to viral RNAs. Recombinant AlMV particles were efficiently produced in tobacco, easily purified for immunological analysis, and exhibited extended stability and systemic proliferation in planta. Intraperitional injections of mice with recombinant plant virus particles harboring the PA-D4s epitope elicited a distinct immune response. Western blotting and ELISA analysis showed that sera from immunized mice recognized both native PA antigen and the AlMV CP. PMID:16236249

  3. Epstein-Barr Virus Encephalitis: A Case Report

    Directory of Open Access Journals (Sweden)

    Somayh HASHEMIAN

    2015-01-01

    and Persistent Epstein-Barr Virus Infection in Pediatric Patients. Journal of Medical Virology 2002; 68:253-63.Young JY, Hyang LK. Transient Asymptomatic White Matter Lesions Following Epstein-Barr virus Encephalitis. Korean pediatric society 2011; 54:389- 93.Doja A, Bitnun A, Jones EL, Richardson S, Tellier R, Petric M, et al. Pediatric Epstein-Barr Virus-Associated Encephalitis:10-Year Review. Child Neurol 2006; 21:385-91.Kou K, Itoh M, Kawano Y. A Case Report of EB Virus- Induced Meningoencephalitis Associated with Brain MRI Abnormalities (basal ganglia. J Japan Peditr Sos 1994; 98:2052-9.Kunlong H, Hung-Tsai L, Minlan T. Epstein-Barr Virus Encephalitis in Children. Acta Pediatrica Taiwanica 2000; 3:140-6.

  4. Co-infections with Chikungunya Virus and Dengue Virus in Delhi, India

    OpenAIRE

    Chahar, Harendra S; Bharaj, Preeti; Dar, Lalit; Guleria, Randeep; Kabra, Sushil K; Broor, Shobha

    2009-01-01

    Aedes aegypti mosquitoes are common vectors for dengue virus and chikungunya virus. In areas where both viruses cocirculate, they can be transmitted together. During a dengue outbreak in Delhi in 2006, 17 of 69 serum samples were positive for chikungunya virus by reverse transcription–PCR; 6 samples were positive for both viruses.

  5. AL-USRCRN Station Information

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Documentation of United States Alabama Regional Climate Reference Network (AL-USRCRN) installations in 2006. Installations documented are for AL-USRCRN pilot...

  6. Nucleotide sequence and phylogenetic analysis of a new potexvirus: Malva mosaic virus.

    Science.gov (United States)

    Côté, Fabien; Paré, Christine; Majeau, Nathalie; Bolduc, Marilène; Leblanc, Eric; Bergeron, Michel G; Bernardy, Michael G; Leclerc, Denis

    2008-01-01

    A filamentous virus isolated from Malva neglecta Wallr. (common mallow) and propagated in Chenopodium quinoa was grown, cloned and the complete nucleotide sequence was determined (GenBank accession # DQ660333). The genomic RNA is 6858 nt in length and contains five major open reading frames (ORFs). The genomic organization is similar to members and the viral encoded proteins shared homology with the group of the Potexvirus genus in the Flexiviridae family. Phylogenetic analysis revealed a close relationship with narcissus mosaic virus (NMV), scallion virus X (ScaVX) and, to a lesser extent, to Alstroemeria virus X (AlsVX) and pepino mosaic virus (PepMV). A novel putative pseudoknot structure is predicted in the 3'-UTR of a subgroup of potexviruses, including this newly described virus. The consensus GAAAA sequence is detected at the 5'-end of the genomic RNA and experimental data strongly suggest that this motif could be a distinctive hallmark of this genus. The name Malva mosaic virus is proposed. PMID:18054524

  7. Ebola Virus Antibodies in Fruit Bats, Bangladesh

    OpenAIRE

    Kevin J Olival; Islam, Ariful; YU, Meng; Anthony, Simon J.; Epstein, Jonathan H.; Khan, Shahneaz Ali; Khan, Salah Uddin; Crameri, Gary; Wang, Lin-Fa; Lipkin, W. Ian; Luby, Stephen P.; Daszak, Peter

    2013-01-01

    To determine geographic range for Ebola virus, we tested 276 bats in Bangladesh. Five (3.5%) bats were positive for antibodies against Ebola Zaire and Reston viruses; no virus was detected by PCR. These bats might be a reservoir for Ebola or Ebola-like viruses, and extend the range of filoviruses to mainland Asia.

  8. Advanced Metamorphic Techniques in Computer Viruses

    OpenAIRE

    Beaucamps, Philippe

    2007-01-01

    Nowadays viruses use polymorphic techniques to mutate their code on each replication, thus evading detection by antiviruses. However detection by emulation can defeat simple polymorphism: thus metamorphic techniques are used which thoroughly change the viral code, even after decryption. We briefly detail this evolution of virus protection techniques against detection and then study the MetaPHOR virus, today's most advanced metamorphic virus.

  9. Derivados vinculados al seguro.

    Directory of Open Access Journals (Sweden)

    De Paz Cobo, Sonia

    2003-01-01

    Full Text Available El presente trabajo tiene como objetivo la presentación de los derivados financieros como instrumentos de cobertura de los riesgos del seguro, así como de los principales proyectos que se desarrollan a través de los mismos, como por ejemplo los derivados sobre el clima. Hay que tener presente las pérdidas multimillonarias ocasionadas en casos como el de la banca Baring’s, o el de Orange County, por citar algunas, que si bien ciertos autores afirman que están en relación directa con el uso de estos productos, no podemos olvidar que los instrumentos derivados se desarrollaron con el objetivo principal de proteger al usuario de la posible volatilidad del activo subyacente. Ya en la industria aseguradora los derivados vinculados a tal actividad son instrumentos donde el pago se condiciona a la ocurrencia de su suceso de siniestro, o serie de sucesos múltiples que disparen las pérdidas. Tras el repaso efectuado y la descripción que este tipo de instrumentos puede tener en la gestión del riesgo por parte de la industria aseguradora, concluiremos con la exposición de las relaciones con el reaseguro tradicional así como una breve referencia al futuro de este tipo de instrumentos

  10. Gliricidia sepium al establecimiento

    Directory of Open Access Journals (Sweden)

    J. L. Valle

    2004-01-01

    Full Text Available Se asoció Cenchrus ciliaris (Cc y Gliricidia sepium (Gs para evaluar la producción de biomasa y la composición química de los forrajes en la fase de establecimiento, en el estado de Morelos, México, en condiciones de trópico seco. Se empleó un análisis de varianza con diseño en bloques al azar, en donde T1 fue pasto solo, T2 Cc más Gs con 5,000 plantas ha-1 y el T3 Cs más Gs con 14,285 plantas ha-1. Se midió la producción de forraje individual y asociado con materia seca (t MS/Ha, la altura (A en cm, la proteína cruda (PC%, las fracciones de fibra (FDN% y FDA% y la digestibilidad in vitro de la materia seca (DIVMS%. El T3 tuvo una mejor producción de biomasa (P0.05. La asociación de 14,285 plantas ha-1 de G. sepium con pasto Cenchrus ciliaris mejoró la producción de biomasa y la disponibilidad total de nutrientes por superficie cultivada al establecimiento

  11. The IFITMs Inhibit Zika Virus Replication.

    Science.gov (United States)

    Savidis, George; Perreira, Jill M; Portmann, Jocelyn M; Meraner, Paul; Guo, Zhiru; Green, Sharone; Brass, Abraham L

    2016-06-14

    Zika virus has emerged as a severe health threat with a rapidly expanding range. The IFITM family of restriction factors inhibits the replication of a broad range of viruses, including the closely related flaviruses West Nile virus and dengue virus. Here, we show that IFITM1 and IFITM3 inhibit Zika virus infection early in the viral life cycle. Moreover, IFITM3 can prevent Zika-virus-induced cell death. These results suggest that strategies to boost the actions and/or levels of the IFITMs might be useful for inhibiting a broad range of emerging viruses. PMID:27268505

  12. The IFITMs Inhibit Zika Virus Replication

    Directory of Open Access Journals (Sweden)

    George Savidis

    2016-06-01

    Full Text Available Zika virus has emerged as a severe health threat with a rapidly expanding range. The IFITM family of restriction factors inhibits the replication of a broad range of viruses, including the closely related flaviruses West Nile virus and dengue virus. Here, we show that IFITM1 and IFITM3 inhibit Zika virus infection early in the viral life cycle. Moreover, IFITM3 can prevent Zika-virus-induced cell death. These results suggest that strategies to boost the actions and/or levels of the IFITMs might be useful for inhibiting a broad range of emerging viruses.

  13. Sweep flocculation and adsorption of viruses on aluminum flocs during electrochemical treatment prior to surface water microfiltration.

    Science.gov (United States)

    Tanneru, Charan Tej; Rimer, Jeffrey D; Chellam, Shankararaman

    2013-05-01

    Bench-scale experiments were performed to evaluate virus control by an integrated electrochemical-microfiltration (MF) process from turbid (15 NTU) surface water containing moderate amounts of dissolved organic carbon (DOC, 5 mg C/L) and calcium hardness (50 mg/L as CaCO3). Higher reductions in MS2 bacteriophage concentrations were obtained by aluminum electrocoagulation and electroflotation compared with conventional aluminum sulfate coagulation. This was attributed to electrophoretic migration of viruses, which increased their concentrations in the microenvironment of the sacrificial anode where coagulant precursors are dissolved leading to better destabilization during electrolysis. In all cases, viruses were not inactivated implying measured reductions were solely due to their removal. Sweep flocculation was the primary virus destabilization mechanism. Direct evidence for virus enmeshment in flocs was provided by two independent methods: quantitative elution using beef extract at elevated pH and quantitating fluorescence from labeled viruses. Atomic force microscopy studies revealed a monotonically increasing adhesion force between viruses immobilized on AFM tips and floc surfaces with electrocoagulant dosage, which suggests secondary contributions to virus uptake on flocs from adsorption. Virus sorption mechanisms include charge neutralization and hydrophobic interactions with natural organic matter removed during coagulation. This also provided the basis for interpreting additional removal of viruses by the thick cake formed on the surface of the microfilter following electrocoagulation. Enhancements in virus removal as progressively more aluminum was electrolyzed therefore embodies contributions from (i) better encapsulation onto greater amounts of fresh Al(OH)3 precipitates, (ii) increased adsorption capacity associated with higher available coagulant surface area, (iii) greater virus-floc binding affinity due to effective charge neutralization and

  14. Defective interfering particles of Sindbis virus do not interfere with the homologous virus obtained from persistently infected BHK cells but do interfere with Semliki Forest virus.

    OpenAIRE

    Weiss, B; Schlesinger, S

    1981-01-01

    Defective interfering particles derived from wild-type Sindbis virus no longer interfere with the infectious virus cloned from BHK cells persistently infected with Sindbis virus for 16 months. These particles do interfere with the replication of Semliki Forest virus.

  15. Sporadic ALS/MND: a global neurodegeneration with retroviral involvement?

    Science.gov (United States)

    Westarp, M E; Ferrante, P; Perron, H; Bartmann, P; Kornhuber, H H

    1995-05-01

    Sporadic amyotrophic lateral sclerosis may be an aetiologically heterogenous disease. We confirmed elevated circulating IgG immune complexes, and altered IgG seroreactivities against human retroviral antigens (HIV-2 and HTLV immunoblots) in overlapping subgroups of patients. Together with preliminary findings of a positive polymerase chain reactivity for human T-lymphotropic virus (HTLV.tax/rex) in blood leukocytes of 5 out of 14 sALS patients, we interpret this as evidence for a retroviral involvement in this relentlessly progressive, often asymmetrically spreading neurodegeneration. The possibility of a secondary phenomenon seems unlikely, yet cannot be completely ruled out. PMID:7595609

  16. Oncolytic virus therapy for cancer

    Directory of Open Access Journals (Sweden)

    Goldufsky J

    2013-09-01

    Full Text Available Joe Goldufsky,1 Shanthi Sivendran,3 Sara Harcharik,4 Michael Pan,4 Sebastian Bernardo,4 Richard H Stern,5 Philip Friedlander,4 Carl E Ruby,1,2 Yvonne Saenger,4 Howard L Kaufman1,2 Departments of 1Immunology & Microbiology and 2Surgery, Rush University Medical Center, Chicago IL, USA 3Hematology/Oncology Medical Specialists, Lancaster General Health, Lancaster, PA, USA, and Departments of 4Medical Oncology and 5Radiology, Tisch Cancer Institute, The Mount Sinai School of Medicine, New York, NY, USA Abstract: The use of oncolytic viruses to treat cancer is based on the selection of tropic tumor viruses or the generation of replication selective vectors that can either directly kill infected tumor cells or increase their susceptibility to cell death and apoptosis through additional exposure to radiation or chemotherapy. In addition, viral vectors can be modified to promote more potent tumor cell death, improve the toxicity profile, and/or generate host antitumor immunity. A variety of viruses have been developed as oncolytic therapeutics, including adenovirus, vaccinia virus, herpesvirus, coxsackie A virus, Newcastle disease virus, and reovirus. The clinical development of oncolytic viral therapy has accelerated in the last few years, with several vectors entering clinical trials for a variety of cancers. In this review, current strategies to optimize the therapeutic effectiveness and safety of the major oncolytic viruses are discussed, and a summary of current clinical trials is provided. Further investigation is needed to characterize better the clinical impact of oncolytic viruses, but there are increasing data demonstrating the potential promise of this approach for the treatment of human and animal cancers. Keywords: cancer, gene therapy, oncolytic therapy, virus, treatment

  17. Neutropenia crónica e infección por el virus de la inmunodeficiencia humana

    OpenAIRE

    Ronald A Noguera-Valverde; Ricardo Boza-Cordero

    2008-01-01

    Se presenta el caso de un paciente masculino con neutropenia crónica e infección por el virus de inmunodeficiencia humana, con una revisión de los posibles mecanismos patogénicos. Las alteraciones hematológicas como anemia, trombocitopenia y leucopenia se presentan asociadas con frecuencia a la infección aguda por el virus de inmunodeficiencia humana. Al establecer la terapia antirretroviral y disminuir la actividad del virus, estas alteraciones tienden a mejorar. Sin embargo, algunos fármaco...

  18. Conserved hemagglutinin peptides of influenza virus as potential multivalent vaccine candidate: characterization of immune response in different animal models

    OpenAIRE

    González Zabala, Juliana

    2016-01-01

    Los virus de influenza A (IAVs) son los responsables de brotes pandémicos y de la mayoría de las epidemias anuales en los seres humanos, aves y cerdos. Los virus de influenza A se dividen en subtipos, basado en la naturaleza de sus glicoproteínas de superficie, hemaglutinina (HA) y neuraminidasa (NA). La HA es una glicoproteína de superficie homotrimérica que media la entrada del virus de influenza a través de la unión celular y eventos de fusión de membranas. El bolsillo de unión al receptor...

  19. A new looming of Zika virus

    OpenAIRE

    Nirav R. Soni

    2016-01-01

    Zika virus (ZIKV) is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by daytime-active Aedes mosquitoes, such as A. aegypti. ZIKV will continue to spread and it will be difficult to determine how the virus will spread over time. Sign and symptoms of ZIKAVD (Zika virus disease) were conjunctivitis (red eyes), back pain, birth defect-abnormal brain development known as microcephaly and it is diagnosed through PCR (polymerase chain reaction) and virus isolation fr...

  20. Towards the Epidemiological Modeling of Computer Viruses

    OpenAIRE

    Xiaofan Yang; Lu-Xing Yang

    2012-01-01

    Epidemic dynamics of computer viruses is an emerging discipline aiming to understand the way that computer viruses spread on networks. This paper is intended to establish a series of rational epidemic models of computer viruses. First, a close inspection of some common characteristics shared by all typical computer viruses clearly reveals the flaws of previous models. Then, a generic epidemic model of viruses, which is named as the SLBS model, is proposed. Finally, diverse generalizations of ...

  1. Virus-membrane interactions: spectroscopic studies.

    OpenAIRE

    Datema, K.P.

    1987-01-01

    In this thesis some new aspects of the infection process of nonenveloped viruses are reported. The interaction of a rod-shaped (TMV) and three spherical (CCMV, BMV, SBMV) plant viruses, of the filamentous bacteriophage M13, and of their coat proteins with membranes have been investigated. A comparison is made between the infection mechanisms of these non-enveloped viruses.1 EFFECT OF PLANT VIRUSES ON MEMBRANESAll plant viruses studied interact with membranes. This is demonstrated by turbidity...

  2. Advanced Virus Monitoring and Analysis System

    OpenAIRE

    Fauzi Adi Rafrastara; Faizal M. A.

    2011-01-01

    This research proposed an architecture and a system which able to monitor the virus behavior and classify them as a traditional or polymorphic virus. Preliminary research was conducted to get the current virus behavior and to find the certain parameters which usually used by virus to attack the computer target. Finally, “test bed environment” is used to test our system by releasing the virus in a real environment, and try to capture their behavior, and followed by generating the conclusion th...

  3. Detection of Junk Instructions in Computer Viruses

    OpenAIRE

    2008-01-01

    The techniques employed by viruses to avoid detection by antivirus scanners are becoming increasingly advanced. One technique commonly used by viruses to evade detection is polymorphism. The level of polymorphism in a virus indicates its ability to create different forms of itself. The use of junk instructions is a common technique to increase the level of polymorphism in a virus. Junk instructions are machine code instructions with no other function than to alter the appearance of a virus. J...

  4. Animal viruses, radiation, repair mechanisms, and cancer

    International Nuclear Information System (INIS)

    Effects of radiation on animal viruses are reviewed in terms of repair mechanisms and their possible relationship to virus transformation and carcinogenesis. Repair mechanisms are reviewed under the headings; multiplicity reactivation, photoreactivation, host-cell reactivation (dark repair), and uv reactivation. Other topics discussed are: transformation of cells in vitro by irradiated viruses, oncogenicity of virus-transformed cells in vivo, and possible role of repair mechanisms in virus transformation and oncogenesis

  5. RNA recombination in animal and plant viruses.

    OpenAIRE

    Lai, M M

    1992-01-01

    An increasing number of animal and plant viruses have been shown to undergo RNA-RNA recombination, which is defined as the exchange of genetic information between nonsegmented RNAs. Only some of these viruses have been shown to undergo recombination in experimental infection of tissue culture, animals, and plants. However, a survey of viral RNA structure and sequences suggests that many RNA viruses were derived form homologous or nonhomologous recombination between viruses or between viruses ...

  6. Zika Virus: the Latest Newcomer

    OpenAIRE

    Saiz, Juan-Carlos; Vázquez-Calvo, Ángela; Blázquez, Ana B.; Merino-Ramos, Teresa; Escribano-Romero, Estela; Martín-Acebes, Miguel A.

    2016-01-01

    Since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: West Nile, Influenza A, avian flu, dengue, Chikungunya, SARS, MERS, Ebola, and now Zika, the latest newcomer. Zika virus (ZIKV), a flavivirus transmitted by Aedes mosquitoes, was identified in 1947 in a sentinel monkey in Uganda, and later on in humans in Nigeria. The virus was mainly confined to the African continent until it was detected in south-east Asia the 1980’s...

  7. Zika virus: the latest newcomer

    OpenAIRE

    Juan-Carlos eSaiz; Angela eVazquez-Calvo; Ana Belen Blazquez; Teresa eMerino-Ramos; Estela eEscribano-Romero; Martín-Acebes, Miguel A.

    2016-01-01

    Since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: West Nile, Influenza A, avian flu, dengue, Chikungunya, SARS, MERS, Ebola, and now Zika, the latest newcomer. Zika virus (ZIKV), a flavivirus transmitted by Aedes mosquitoes, was identified in 1947 in a sentinel monkey in Uganda, and later on in humans in Nigeria. The virus was mainly confined to the African continent until it was detected in south-east Asia the 1980´s...

  8. Marburg Virus Reverse Genetics Systems

    Directory of Open Access Journals (Sweden)

    Kristina Maria Schmidt

    2016-06-01

    Full Text Available The highly pathogenic Marburg virus (MARV is a member of the Filoviridae family and belongs to the group of nonsegmented negative-strand RNA viruses. Reverse genetics systems established for MARV have been used to study various aspects of the viral replication cycle, analyze host responses, image viral infection, and screen for antivirals. This article provides an overview of the currently established MARV reverse genetic systems based on minigenomes, infectious virus-like particles and full-length clones, and the research that has been conducted using these systems.

  9. Epitaxial growth of Al/Al2O3 multilayer structures

    International Nuclear Information System (INIS)

    In order to obtain Al-Al2O3 quantum effect devices, it is necessary to deposit epitaxially Al on Al2O3 and Al2O3 on Al. We have already obtained an epitaxial Al/Al2O3/Al structures on Si(111). In this paper, we have deposit Al/Al2O3/Al structures on Si(111), and investigated the crystallinity by using RBS. (author)

  10. MODEL DINAMIKA VIRUS HIV DAN VIRUS MUTAN DENGAN SISTEM IMUN

    OpenAIRE

    Ikhsan, Laode Muhammad

    2014-01-01

    Model of the dynamics of HIV infection with the mutan virus and the Immune System is a mathematical model that describes the interactions between healthy T cells, in infected cells, and the HIV virus. The models obtained three equilibrium points, which has an endemic equilibrium point and the point of equilibrium without the disease. By doing stability analysis, the stability of the equilibrium point has obtained depends on the model of the basic reproduction number (R01 dan R02). For the...

  11. Zika virus infections.

    Science.gov (United States)

    de Laval, F; Leparc-Goffart, I; Meynard, J-B; Daubigny, H; Simon, F; Briolant, S

    2016-05-01

    Since its discovery in 1947 in Uganda, the Zika virus (ZIKV) remained in the shadows emerging in 2007 in Micronesia, where hundreds of dengue-like syndromes were reported. Then, in 2013-2014, it was rife in French Polynesia, where the first neurological effects were observed. More recently, its arrival in Brazil was accompanied by an unusually high number of children with microcephaly born to mothers infected with ZIKV during the first trimester of pregnancy. In 2016, the World Health Organization declared ZIKV infection to be a public health emergency and now talks about a ZIKV pandemic. This review aims to summarize the current knowledge about ZIKV infection, successively addressing its transmission, epidemiology, clinical aspects, diagnosis, treatment, and prevention before discussing some perspectives. PMID:27412976

  12. Doppelthydrophile Blockcopolymere als Mineralisationstemplate

    Science.gov (United States)

    Kasparova, Pavla

    2002-07-01

    Die vorliegende Arbeit beschäftigt sich mit der Synthese und den Eigenschaften von doppelthydrophilen Blockcopolymeren und ihrer Anwendung in einem biomimetischen Mineralisationsprozeß von Calciumcarbonat und Bariumsulfat. Doppelthydrophile Blockcopolymere bestehen aus einem hydrophilen Block, der nicht mit Mineralien wechselwirkt und einem zweiten Polyelektrolyt-Block, der stark mit Mineraloberflächen wechselwirkt. Diese Blockcopolymere wurden durch ringöffnende Polymerisation von N-carboxyanhydriden (NCA's) und a-methoxy-ω-amino[poly(ethylene glycol)] PEG-NH2 als Initiator hergestellt. Die hergestellten Blockcopolymere wurden als effektive Wachstumsmodifikatoren für die Kristallisation von Calciumcarbonat und Bariumsulfat Mineralien eingesetzt. Die so erhaltenen Mineralpartikel (Kugeln, Hantel, eiförmige Partikel) wurden durch Lichtmikroskopie in Lösung, SEM und TEM charakterisiert. Röntgenweitwinkelstreuung (WAXS) wurde verwendet, um die Modifikation von Calciumcarbonat zu ermitteln und die Größe der Calciumcarbonat- und Bariumsulfat-Nanopartikel zu ermitteln. This work describes the synthesis and characterization of double hydrophilic block copolymers and their use in a biomimetic mineralization process of Calcium Carbonate and Barium Sulfate. Double hydrophilic block copolymers consist of a hydrophilic block that does not interact with minerals and another hydrophilic polyelectrolyte block that strongly interacts with mineral surfaces. These polymers were synthesised via ring opening polymerisation of N-carboxyanhydride (NCA), and the first hydrophilic block a-methoxy-ω-amino[poly(ethylene glycol)] PEG-NH2 was used as an initiator. The prepared block copolymers were used as effective crystal growth modifiers to control the crystallization of Calcium Carbonate and Barium Sulfate minerals. The resulting mineral particles (spheres, dumbbells, egg-like particles) were characterised by light microscopy in solution, by SEM, and by TEM. X-Ray scattering

  13. Cryptovirology: Virus Approach

    Directory of Open Access Journals (Sweden)

    Shivale Saurabh Anandrao

    2011-08-01

    Full Text Available Traditionally, "Cryptography" is a benediction to information processing and communications, it helps people to store information securely and the private communications over long distances. Cryptovirology is the study of applications of cryptography to build the malicious software. It is an investigation, how modern cryptographic tools and paradigms can be used to strengthen, develop and improve new malicious software attacks. Cryptovirology attacks have been categorized as : give malware enhanced privacy andbe more robust against reverse-engineering, secondly give the attacker enhanced anonymity while communicating with deployed malware. This paper presents the idea of ``Cryptovirology'' which introduce a twist on how cryptography can also be used offensively. Being offensive means, it can be used to mount extortion based attacks that cause loss of access to information, loss of confidentiality, and information leakage, tasks which cryptography usually prevents. Also analyze threats and attacks that misuse of cryptography can cause when combined with fraudulent software (viruses, Trojans. Public-keycryptography is very essential for the attacks that based on cryptovirology. This paper also suggest some of the countermeasures, mechanisms to cope with and prevent such attacks. Even if the attackers actions on the host machine are being monitored, it still cannot be proven beyond reasonable doubt that he or she is the attacker; and it is an “originator-concealing attack”. Evidence should be collected from the “author’s own system which was used for the attack”. These attacks have implications on how the use ofcryptographic tools and techniques should be audited and managed in general purpose computing environments, and imply that access to the cryptographic tools should be in well control of the system(such as API routines. The experimental virus would demonstrate how cryptographic packages can be packed into a small space, which may

  14. Fretting wear performances of multilayered PVD TiAlZrN/TiAlN/TiAl on AISI 4140 steel

    OpenAIRE

    Tlili, Brahim; NASRI, Mustapha; F. Ayari; Nouveau, Corinne

    2008-01-01

    Nowadays, most surface treatments are realized through vapor deposition techniques as thin hard coatings to guarantee; high surface hardness, low friction coefficient and improve wear resistance. Several experimental investigations have let to the development of a (TiAlCN/TiAlN/TiAl) and (TiAlZrN/TiAlN/TiAl) coatings in preference to the traditional TiN coating. In the current paper research conducted on fretting wear of a (TiAlCN/TiAlN/TiAl) and a (TiAlZrN/TiAlN/TiAl) multilayer coatings de...

  15. Adeno-associated virus: from defective virus to effective vector

    Directory of Open Access Journals (Sweden)

    Gonçalves Manuel AFV

    2005-05-01

    Full Text Available Abstract The initial discovery of adeno-associated virus (AAV mixed with adenovirus particles was not a fortuitous one but rather an expression of AAV biology. Indeed, as it came to be known, in addition to the unavoidable host cell, AAV typically needs a so-called helper virus such as adenovirus to replicate. Since the AAV life cycle revolves around another unrelated virus it was dubbed a satellite virus. However, the structural simplicity plus the defective and non-pathogenic character of this satellite virus caused recombinant forms to acquire centre-stage prominence in the current constellation of vectors for human gene therapy. In the present review, issues related to the development of recombinant AAV (rAAV vectors, from the general principle to production methods, tropism modifications and other emerging technologies are discussed. In addition, the accumulating knowledge regarding the mechanisms of rAAV genome transduction and persistence is reviewed. The topics on rAAV vectorology are supplemented with information on the parental virus biology with an emphasis on aspects that directly impact on vector design and performance such as genome replication, genetic structure, and host cell entry.

  16. Transfection by DNAs of avian erythroblastosis virus and avian myelocytomatosis virus strain MC29.

    OpenAIRE

    Copeland, N G; Cooper, G M

    1980-01-01

    Chicken embryo fibroblasts and NIH 3T3 mouse cells were transformable by DNAs of chicken cells infected with avian myelocytomatosis virus strain MC29 or with avian erythroblastosis virus. Transfection of chicken cells appeared to require replication of MC29 or avian erythroblastosis virus in the presence of a nontransforming helper virus. In contrast, NIH 3T3 cells transformed by MC29 or avian erythroblastosis virus DNA contained only replication-defective transforming virus genomes.

  17. Varicella-zoster virus vaccine DNA differs from the parental virus DNA.

    OpenAIRE

    Ecker, J R; Hyman, R W

    1981-01-01

    The DNAs of a varicella-zoster virus vaccine and its parental virus were compared by CsCl buoyant density centrifugation and restriction enzyme cleavage analysis. The varicella-zoster virus vaccine DNA showed a heterogeneous buoyant profile and altered restriction enzyme cleavage patterns. These changed properties are probably the result of the accumulation of virus containing defective varicella-zoster virus DNA during extensive cell culture passage of the vaccine virus.

  18. Neoplastic transformation of chimpanzee cells induced by adenovirus type 12--simian virus 40 hybrid virus.

    OpenAIRE

    Rhim, J S; Trimmer, R; Arnstein, P; Huebner, R J

    1981-01-01

    The adenovirus 12--simian virus 40 hybrid virus produced neoplastic transformation of chimpanzee skin fibroblasts in vitro. The transformed fibroblasts showed morphological alteration and became permanent lines. The transformed cells contained both adenovirus 12 and simian virus 40 large tumor antigens and were virus producers. However at passage 9, one line (WES) was found to be a nonproducer, producing neither infectious virus nor virus-specific antigen detectable by the complement fixation...

  19. Potential of acylated peptides to target the influenza A virus

    Directory of Open Access Journals (Sweden)

    Daniel Lauster

    2015-04-01

    Full Text Available For antiviral drug design, especially in the field of influenza virus research, potent multivalent inhibitors raise high expectations for combating epidemics and pandemics. Among a large variety of covalent and non-covalent scaffold systems for a multivalent display of inhibitors, we created a simple supramolecular platform to enhance the antiviral effect of our recently developed antiviral Peptide B (PeBGF, preventing binding of influenza virus to the host cell. By conjugating the peptide with stearic acid to create a higher-order structure with a multivalent display, we could significantly enhance the inhibitory effect against the serotypes of both human pathogenic influenza virus A/Aichi/2/1968 H3N2, and avian pathogenic A/FPV/Rostock/34 H7N1 in the hemagglutination inhibition assay. Further, the inhibitory potential of stearylated PeBGF (C18-PeBGF was investigated by infection inhibition assays, in which we achieved low micromolar inhibition constants against both viral strains. In addition, we compared C18-PeBGF to other published amphiphilic peptide inhibitors, such as the stearylated sugar receptor mimicking peptide (Matsubara et al. 2010, and the “Entry Blocker” (EB (Jones et al. 2006, with respect to their antiviral activity against infection by Influenza A Virus (IAV H3N2. However, while this strategy seems at a first glance promising, the native situation is quite different from our experimental model settings. First, we found a strong potential of those peptides to form large amyloid-like supramolecular assemblies. Second, in vivo, the large excess of cell surface membranes provides an unspecific target for the stearylated peptides. We show that acylated peptides insert into the lipid phase of such membranes. Eventually, our study reveals serious limitations of this type of self-assembling IAV inhibitors.

  20. A recombinant rabies virus expressing vesicular stomatitis virus glycoprotein fails to protect against rabies virus infection

    OpenAIRE

    Foley, Heather D.; McGettigan, James P.; Siler, Catherine A.; Dietzschold, Bernhard; Schnell, Matthias J.

    2000-01-01

    To investigate the importance of the rabies virus (RV) glycoprotein (G) in protection against rabies, we constructed a recombinant RV (rRV) in which the RV G ecto- and transmembrane domains were replaced with the corresponding regions of vesicular stomatitis virus (VSV) glycoprotein (rRV-VSV-G). We were able to recover rRV-VSV-G and found that particle production was equal to rRV. However, the budding of the chimeric virus was delayed and infectious titers were red...

  1. Zika virus: An international emergency?

    Science.gov (United States)

    Palomo, Adolfo Martinez

    2016-05-01

    This Viewpoint discusses the World Health Organization's Declaration on 1 February 2016 that the epidemic infection caused by the Zika virus is a public health emergency of international concern - the basis of the decision and controversy surrounding it. PMID:26911655

  2. Oncolytic viruses in radiation oncology

    International Nuclear Information System (INIS)

    Oncolytic viruses are investigational cancer treatments. They are currently being assessed as single agents or in combination with standard therapies such as external beam radiotherapy - a DNA damaging agent that is a standard of care for many tumour types. Preclinical data indicate that combinations of oncolytic viruses and radiation therapy are promising, showing additional or synergistic antitumour effects in in vitro and in vivo studies. This interaction has the potential to be multifaceted: viruses may act as radiosensitizing agents, but radiation may also enhance viral oncolysis by increasing viral uptake, replication, gene expression and cell death (apoptosis, autophagy or necrosis) in irradiated cells. Phase I and II clinical trials investigating combinations of viruses and radiation therapy have been completed, paving the way for ongoing phase III studies. The aim of this review is to focus on the therapeutic potential of these combinations and to highlight their mechanistic bases, with particular emphasis on the role of the DNA damage response.

  3. Dengue Virus Infection in Africa

    OpenAIRE

    Amarasinghe, Ananda; Kuritsky, Joel N.; Letson, G. William; Margolis, Harold S

    2011-01-01

    Reported incidence of dengue has increased worldwide in recent decades, but little is known about its incidence in Africa. During 1960–2010, a total of 22 countries in Africa reported sporadic cases or outbreaks of dengue; 12 other countries in Africa reported dengue only in travelers. The presence of disease and high prevalence of antibody to dengue virus in limited serologic surveys suggest endemic dengue virus infection in all or many parts of Africa. Dengue is likely underrecognized and u...

  4. Hepatitis virus vaccines: present status.

    OpenAIRE

    Krugman, S.

    1982-01-01

    During the past decade there has been extraordinary progress toward the development of vaccines for the prevention of type A and type B hepatitis. The successful propagation of hepatitis A virus in cell culture in 1979 was followed by the preparation of experimental live attenuated hepatitis A vaccines that have been shown to induce antibody in marmosets and chimpanzees and protect immunized marmosets against challenge with hepatitis A virus. The first human immunization trials will begin in ...

  5. Malamih al-Ashwat al-'Arabiyyah wa Makharijaha

    Directory of Open Access Journals (Sweden)

    Amrah Muhammad Kasim

    2015-12-01

    Full Text Available This writing discusses on al-ashwat al-‘arabiyyah that is known with “phonetic” term. It talks and interprets makharij al-huruf or place for producing sounds and also processing production of sounds by parts of mouth in Arabic language. The problems of sounds and its systems in Arabic language have been conducted by the scholars of al-ashwat al-‘arabiyyah attractively, neatly, and detail and gotten ahead the phonetic western scholars. Part of their analysis results formulate the study about al-ashwat al-sakinah (al-Shamit is known with vocal and consonant sounds in research questions shawait – shawamit. Ibnu Jinni has formulated “harakat”(wowel in Arabic language and dimension of letter  mad and liyn on alif  letter - waw - ya’ (ا و ي, from these letters create  harakat fathah, dammah, and kasrah. Moreover, in this writing also exist the description of hija’i letters and its makhraj that are correct from parts of mouth and the unity of coordinative sounds that produce sounds in morphemes arrangement, in one word that produces meaning. The result of phonological study has divided al-ashwat to be kinds of sound like the pronounced sounds, produced sounds from mouth, and heard sound.

  6. Hendra Virus Re-visited

    Institute of Scientific and Technical Information of China (English)

    Hume Field

    2009-01-01

    Hendra virus, a novel member of the family Paramyxovirus that has emerged from bats in Australia, causes fatal disease in livestock and humans. Eleven spillover events have been identified since the first description of the virus in 1994, resulting in a total of 37 equine cases and six human cases. All human cases have been attributed to exposure to infected horses; there is no evidence of bat-to-human or human-to-human transmission. Low infectivity and a high case fatality rate are features of Hendra virus infection in both horses and humans. The temporal pattern of spillover events suggests seasonal factors (plausibly be environmental, biological or ecological) as the proximate triggers for spillover. Minimisation of the future occurrence and impact of Hendra virus infections requires an understanding of the ecology of flying foxes, of virus infection dynamics in flying foxes, and of the factors that promote spillover. Management strategies seek to minimize the opportunity for effective contact between bats and horses, and limit potential horse-to-horse and horse-to-human transmission. Incomplete knowledge of the ecology of the virus, of the proximate factors associated with spillover, and the inherent difficulties of effectively managing wild populations, preclude a management approach targeted at bats.

  7. Editorial: Cartas al Editor

    Directory of Open Access Journals (Sweden)

    Guillermo Llanos

    2006-06-01

    Full Text Available En todas las revistas biomédicas del mundo existe una sección habitual denominada como el título de este editorial y que en Colombia Médica la llamamos correspondencia. Este espacio tiene como objetivo fundamental que los lectores escriban a los autores (investigadores comentando los artículos que se han publicado en números anteriores de la revista. Las cartas son, entonces, breves comentarios de desacuerdo o acuerdo (conceptual, metodológico, interpretativo sobre falencias ubicadas por un lector alerta al tema debatido en cualquiera de las secciones como en los artículos originales, editoriales, revisiones, presentación de casos, etc. publicados previamente en la revista. Las cartas deben permitir el intercambio de opiniones y formas de pensar y analizar los problemas planteados entre los autores y los lectores. En ocasiones una serie de cartas motivadas por un artículo original puede ser tan o más interesante como el artículo que generó la correspondencia. Las cartas son también un formato adecuado para comunicar un hecho novedoso de una observación clínica juiciosa no descrita antes. Un ejemplo de ello es la carta de un gineco-obstetra australiano cuando informó a Lancet los primeros efectos adversos informados de la talidomida. Colombia Médica, a pesar de mantener vigente esta sección, no ha sido muy afortunada en establecer una buena cultura del envío de cartas al editor. Pero últimamente han llegado juiciosas observaciones a las cuales se les ha dado prioridad en su publicación. Toda carta que comenta los hallazgos de un artículo es enviada a los autores para su respuesta, porque ello genera el debate académico buscado, y luego se publican, tanto la carta como su respuesta. El Estilo Vancouver recomienda que la carta y su respuesta deban ser breve, no pasar de dos o tres párrafos y contener sólo cuatro o cinco referencias. En lo posible (a menos que sea indispensable no presentar ni cuadros ni gráficos. El t

  8. New features in the genus Ilarvirus revealed by the nucleotide sequence of Fragaria chiloensis latent virus.

    Science.gov (United States)

    Tzanetakis, Ioannis E; Martin, Robert R

    2005-09-01

    Fragaria chiloensis latent virus (FClLV), a member of the genus Ilarvirus was first identified in the early 1990s. Double-stranded RNA was extracted from FClLV infected plants and cloned. The complete nucleotide sequence of the virus has been elucidated. RNA 1 encodes a protein with methyltransferase and helicase enzymatic motifs while RNA 2 encodes the viral RNA dependent RNA polymerase and an ORF, that shares no homology with other Ilarvirus genes. RNA 3 codes for movement and coat proteins and an additional ORF, making FClLV possibly the first Ilarvirus encoding a third protein in RNA 3. Phylogenetic analysis reveals that FClLV is most closely related to Prune dwarf virus, the type member of subgroup 4 of the Ilarvirus genus. FClLV is also closely related to Alfalfa mosaic virus (AlMV), a virus that shares many properties with Ilarviruses . We propose the reclassification of AlMV as a member of the Ilarvirus genus instead of being a member of a distinct genus. PMID:15878214

  9. Hepatitis C virus and human immunodeficiency virus transmission routes: Differences and similarities.

    Science.gov (United States)

    Cainelli, Francesca

    2013-05-27

    Bouare et al found that hepatitis C virus (HCV) infection in Malian women is mainly transmitted through medical procedures with contaminated supplies, and that human immunodeficiency virus (HIV) transmission is predominantly sexual. The results of this study confirm those of a recent case-control study in New York and Oregon which demonstrated that healthcare exposures represent an important source of new HCV infections in United States. HCV seroprevalence was only 0.2% in pregnant, young Malian women, indicating that hygiene improved in healthcare facilities over time. Heterosexual transmission of HCV is exceptional, and can occur, from males to females, in extremely rare occasions in case of vaginal mucosal damage or less rarely through anal intercourse. The Malian study did not show an association between HIV infection and hospitalization, transfusion, tattoo, dental care. Transmission by needle-stick injury occurs in 0.9%-2.2% of exposures from HCV-infected subjects and in 0.1%-0.3% of exposures from HIV-infected individuals. HCV is therefore more transmissible through percutaneous exposure. PMID:23717734

  10. Zika Virus Infection and Zika Fever: Frequently Asked Questions

    Science.gov (United States)

    ... Frequently asked questions Updated: 25 March 2016 ABOUT ZIKA What is Zika virus infection? Zika virus infection is caused by ... possible to characterize the disease better. How is Zika virus transmitted? Zika virus is transmitted to people ...

  11. A BRIEF REVIEW ON "MOLECULAR DETECTION AND CHARACTERIZATION OF YELLOW MOSAIC VIRUS (YMV INFECTING BLACKGRAM"

    Directory of Open Access Journals (Sweden)

    S.Obaiah

    2013-12-01

    Full Text Available Blackgram (Vigna mungo (L. Hepper is one of the major pulse crops of the tropics and sub tropics. It is the third major pulse crop cultivated in the Indian subcontinent. Pulses and grain legumes are major sources of dietary protein. These crops are subjected to yellow mosaic and golden mosaic diseases caused by white fly transmitted geminiviruses (WTG’s or begomovirus. Of these viruses, mungbean yellow mosaic virus (MYMV is an important one, and it infects five major leguminous species, such as blackgram, greengram, Frenchbean, pigeonpea and soybean causing an annual yield loss of about US $ 300 million (Varma et al., 1992. The MYMV causes 85-100 per cent yield loss in the plants that are infected at the seedling stage (Nene, 1973.MYMV was first observed in Delhi in the late fifties (Nariani, 1960. Virus particles were first observed by Thongmeearkom et al. (1981 and purified by Honda et al. (1983. Hence the characterisation of Yellow Mosaic Virus is essential to study the variability and to identify any new strains/ variants of YMV prevalent in India and Abroad at molecular level for developing the new resistant genotypes.

  12. Hepatitis B Virus Infection in Iran: Current Knowledge, Future Plans

    OpenAIRE

    Hossein Khedmat; Saeed Taheri

    2009-01-01

    Dear Editor,.We greatly enjoyed reading the excellent review article by Alavian, et al., (1) on the epidemiology of hepatitis B virus (HBV) infection in Iranian general population. Reviewing over 250 related articles in this paper as well as inclusion of non-published reports from libraries of different universities of all parts of the country, has made the paper so perfect that one may find any data derived from different studies on the epidemiology of HBV infection in Iran within this artic...

  13. The IFITMs Inhibit Zika Virus Replication

    OpenAIRE

    George Savidis; Jill M. Perreira; Jocelyn M. Portmann; Paul Meraner; Zhiru Guo; Sharone Green; Abraham L. Brass

    2016-01-01

    Zika virus has emerged as a severe health threat with a rapidly expanding range. The IFITM family of restriction factors inhibits the replication of a broad range of viruses, including the closely related flaviruses West Nile virus and dengue virus. Here, we show that IFITM1 and IFITM3 inhibit Zika virus infection early in the viral life cycle. Moreover, IFITM3 can prevent Zika-virus-induced cell death. These results suggest that strategies to boost the actions and/or levels of the IFITMs mig...

  14. Advances in Research of Garlic Virus Diseases

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Garlic virus infection is an important disease which affects garlic production,with the increasing years of planting,harm of virus is serious year by year,which seriously affect yield and quality of garlic.In order to know the garlic virus effectively,the paper reviewed the research situation of several important garlic virus in virus species,origin,distribution,host range,symptom,route of transmission,classification,genome and detection technique and the prevention technology of garlic viruses.At the same ...

  15. The Mode of Net-Virus Actions

    Institute of Scientific and Technical Information of China (English)

    ShanXiuming; LiYing; JiaoJian; LiuYang; RenYong; QiuBen; CaoYiqun

    2004-01-01

    The computer network is not only of benefit to people, but also helpful for the spreading of viruses. The net-viruses spread more widely and rapidly than the traditional viruses, and network has become the major path of the virus spreading. The author analyzes the mode of the net-virus spreading, including E-mail spreading, positive scanning spreading and through-server spreading. Then some defense strategies in the anti-virus field are introduced, and some countermeasures of the net computer users are discussed.

  16. Production of cattle immunotolerant to bovine viral diarrhea virus.

    OpenAIRE

    McClurkin, A W.; Littledike, E T; Cutlip, R C; Frank, G H; Coria, M F; Bolin, S R

    1984-01-01

    Inoculation of bovine virus diarrhea virus into 58 to 125 day old fetuses of bovine virus diarrhea virus seropositive pregnant cows, or inoculation of bovine virus diarrhea virus into seronegative cows 42 to 114 days pregnant, may produce clinically normal calves which are persistently infected with the specific isolate of bovine virus diarrhea virus yet seronegative to the homologous and heterologous isolates. Reinoculation of these persistently infected cattle with their homologous isolate ...

  17. Presence and Distribution of Tobacco Viruses in Montenegro

    OpenAIRE

    Jelena Zindović; Nataša Dukić; Aleksandra Bulajić; Jelena Latinović; Ivana Đekić; Bojan Duduk; Branka Krstić

    2007-01-01

    Seven important tobacco viruses were investigated in Montenegro in 2005: Tobacco Mosaic Virus (TMV), Tomato Spotted Wilt Virus (TSWV), Cucumber Mosaic Virus (CMV), Potato Virus Y (PVY), Alfalfa Mosaic Virus (AMV), Tobacco Ring Spot Virus (TRSV) and Potato Virus X(PVX). This investigation included sample collection from four tobacco growing regions in Montenegro and their serological testing by DAS-ELISA test. Presence of different strains of PVY was investigated as well using DAS ELISA test w...

  18. Peptide inhibitors of dengue virus and West Nile virus infectivity

    Directory of Open Access Journals (Sweden)

    Garry Robert F

    2005-06-01

    Full Text Available Abstract Viral fusion proteins mediate cell entry by undergoing a series of conformational changes that result in virion-target cell membrane fusion. Class I viral fusion proteins, such as those encoded by influenza virus and human immunodeficiency virus (HIV, contain two prominent alpha helices. Peptides that mimic portions of these alpha helices inhibit structural rearrangements of the fusion proteins and prevent viral infection. The envelope glycoprotein (E of flaviviruses, such as West Nile virus (WNV and dengue virus (DENV, are class II viral fusion proteins comprised predominantly of beta sheets. We used a physio-chemical algorithm, the Wimley-White interfacial hydrophobicity scale (WWIHS 1 in combination with known structural data to identify potential peptide inhibitors of WNV and DENV infectivity that target the viral E protein. Viral inhibition assays confirm that several of these peptides specifically interfere with target virus entry with 50% inhibitory concentration (IC50 in the 10 μM range. Inhibitory peptides similar in sequence to domains with a significant WWIHS scores, including domain II (IIb, and the stem domain, were detected. DN59, a peptide corresponding to the stem domain of DENV, inhibited infection by DENV (>99% inhibition of plaque formation at a concentrations of 99% inhibition at

  19. Análisis de datos sobre virus Informáticos

    OpenAIRE

    Dinkova, Zhaneta

    2013-01-01

    El trabajo fin de grado que se presenta en este documento trata de “Aplicar técnicas de Data Mining a un conjunto de datos procedentes de ataques de virus informáticos interceptados en servidores de Internet”. La propuesta de este trabajo surgió de una Institución con el fin de extraer información de un conjunto de datos proveniente de ejecuciones de virus informáticos. Lamentablemente, debido a fuertes restricciones de privacidad por parte de esta Institución y así como al relevo de la perso...

  20. Método de identificación del tropismo del virus VIH

    OpenAIRE

    González-Serna, Manuel Alejandro; Leal, Manuel; Ruiz-Mateos, Ezequiel

    2009-01-01

    La presente invención se refiere a un método de identificación del tropismo del virus de la inmunodeficiencia humana (VIH) en pacientes infectados con dicho virus. El método comprende el cocultivo de células aisladas mononucleares de sangre periférica (PBMCs) de un paciente infectado con el VIH y PBMCs de un sujeto control sano, donde se deplecionan las células PBMCs CD8 positivas del paciente infectado con VIH y/o del sujeto control sano. Los viriones liberados al medio de cultivo s...

  1. Alexandria (Al Iskandariya), Egypt

    Science.gov (United States)

    2002-01-01

    This image of Alexandria was taken by astronauts on board the International Space Station in December 2000 using an Electronic Still Camera. A wider-angle view (STS088-739-90) taken from the Space Shuttle in December 1998 is available for context. Alexandria (Al Iskandariya) occupies a T-shaped peninsula and strip of land separating the Mediterranean from Lake Mariout. Originally the town was built upon a mole (stone breakwater) called Heptastadium, which joined the island of Pharos (see referenced website, below) to the mainland. Since then sedimentary deposits have widened the mole. Since 1905, when the 370,000 Alexandrians lived in an area of about 4 sq km between the two harbors, the city (population 4 million; see referenced website, below) has grown beyond its medieval walls and now occupies an area of about 300 sq km. The Mahmudiya Canal, connecting Alexandria with the Nile, runs to the south of the city and, by a series of locks, enters the harbor of the principal port of Egypt (note ships). The reddish and ochre polygons west of Lake Mariout are salt-evaporation, chemical-storage, and water-treatment ponds within the coastal lagoon. Reference Youssef Halim and Fatma Abou Shouk, 2000, Human impacts on Alexandria's marine environment: UNESCO, Coastal Regions and Small Islands Unit (CSI), Coastal Management Sourcebooks 2 (accessed December 20, 2000) Additional photographs taken by astronauts can be viewed at NASA-JSC's Gateway to Astronaut Photography of Earth. Image ISS001-ESC-5025 provided by the Earth Sciences and Image Analysis Laboratory, Johnson Space Center.

  2. Undulators at the ALS

    International Nuclear Information System (INIS)

    At Lawrence Berkeley Laboratory's (LBL) Advanced Light Source (ALS), three 4.6 m long undulators have been completed, tested and installed. A fourth is under construction. The completed undulators include two 5.0 cm period length, 89 period devices (U5.0s) which achieve a 0.85 T effective field at a 14 mm minimum gap and a 8.0 cm period length, 55 period device (U8.0) that reaches a 1.2 T effective field at a 14 mm minimum gap. The undulator under construction is a 10.0 cm period length, 43 period device (U10.0) that is designed to achieve 0.98 T at a 23 mm gap. Undulator magnetic gap variation (rms) is within 25 microns over the periodic structure length. Reproducibility of the adjustable magnetic gap has been measured to be within +/- 5 microns. Gap adjusting range is from 14 mm to 210 mm, which can be scanned in one minute. The 5.1 m long vacuum chambers are flat in the vertical direction to within 0.74 mm and straight in the horizontal direction to within 0.08 mm over the 4.6 m magnetic structure sections. Vacuum chamber base pressures after UHV beam conditioning are. in the mid 10-11 Torr range and storage ring operating pressures with full current are in the low 10-10 Torr range. Measurements show that the uncorrelated magnetic field errors are 0.23%, and 0.20% for the two U5.Os and the U8.0 respectively and that the field integrals are small over the 1 cm by 6 cm beam aperture. Device description, fabrication, and measurements are presented

  3. Virus elimination in acute lymphocytic choriomeningitis virus infection. Correlation with virus-specific delayed-type hypersensitivity rather than cytotoxicity

    DEFF Research Database (Denmark)

    Thomsen, Allan Randrup; Volkert, M; Bro-Jørgensen, K

    1983-01-01

    The immunological effector mechanism responsible for the elimination of virus in murine acute non-fatal extracranial lymphocytic choriomeningitis virus infection was studied. In this infection virus clearance is generally regarded as the result of a direct action of virus-specific cytotoxic T cells...... (Tc cells) on virus-producing target cells in the infected mouse. However, by manipulating the antiviral immune response by pretreatment with various doses of cyclophosphamide, we found lack of correlation between Tc-cell activity and the clearance of virus. In contrast, we observed a conspicuous...... correlation between the host's ability to mount a virus-specific delayed-type hypersensitivity (DTH) response and its capacity to combat virus. Moreover, pretreatment with silica and carrageenan prolonged viraemia without impairment of the peak Tc-cell response. These findings indicate that Tc cells have...

  4. Ebola virus: recommendations

    CERN Multimedia

    CERN Medical Service

    2014-01-01

    The CERN Medical Service has been closely following, in particular via the WHO, the development of the Ebola virus outbreak currently affecting some African countries. This infectious disease may be passed on through direct contact with the bodily fluids of a sick person.   Based on the recommendations of the WHO and the two Host States, Switzerland and France, as updated on their respective websites, so far there has been no ban on travel to the countries concerned. However, unless it is absolutely essential, you are advised not to visit any of the countries affected by Ebola (Guinea, Republic of Sierra Leone, Liberia, Nigeria). The two Host States have established an alert system, and a check is carried out on departure from the airports of those countries. It is strongly recommended that you contact the Medical Service if you are travelling to those countries. We remind you to observe the basic rules of hygiene such as frequent hand washing, whatever your destination. The Medical Service is...

  5. Hepatitis B virus replication

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Hepadnaviruses, including human hepatitis B virus (HBV), replicate through reverse transcription of an RNA intermediate, the pregenomic RNA (pgRNA). Despite this kinship to retroviruses, there are fundamental differences beyond the fact that hepadnavirions contain DNA instead of RNA. Most peculiar is the initiation of reverse transcription: it occurs by protein-priming, is strictly committed to using an RNA hairpin on the pgRNA,ε, as template, and depends on cellular chaperones;moreover, proper replication can apparently occur only in the specialized environment of intact nucleocapsids.This complexity has hampered an in-depth mechanistic understanding. The recent successful reconstitution in the test tube of active replication initiation complexes from purified components, for duck HBV (DHBV),now allows for the analysis of the biochemistry of hepadnaviral replication at the molecular level. Here we review the current state of knowledge at all steps of the hepadnaviral genome replication cycle, with emphasis on new insights that turned up by the use of such cellfree systems. At this time, they can, unfortunately,not be complemented by three-dimensional structural information on the involved components. However, at least for the s RNA element such information is emerging,raising expectations that combining biophysics with biochemistry and genetics will soon provide a powerful integrated approach for solving the many outstanding questions. The ultimate, though most challenging goal,will be to visualize the hepadnaviral reverse transcriptase in the act of synthesizing DNA, which will also have strong implications for drug development.

  6. Hepatitis B virus morphogenesis

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The hepatitis B virus (HBV) particle consists of an envelope containing three related surface proteins and probably lipid and an icosahedral nucleocapsid of approximately 30 nm diameter enclosing the viral DNA genome and DNA polymerase. The capsid is formed in the cytosol of the infected cell during packaging of an RNA pregenome replication complex by multiple copies of a 21-kDa C protein. The capsid gains the ability to bud during synthesis of the viral DNA genome by reverse transcription of the pregenome in the lumen of the particle. The three envelope proteins S,M, and L shape a complex transmembrane fold at the endoplasmic reticulum, and form disulfide-linked homoand heterodimers. The transmembrane topology of a fraction of the large envelope protein L changes posttranslationally, therefore, the N terminal domain of L (preS) finally appears on both sides of the membrane.During budding at an intracellular membrane, a short linear domain in the cytosolic preS region interacts with binding sites on the capsid surface. The virions are subsequently secreted into the blood. In addition, the surface proteins can bud in the absence of capsids and form subviral lipoprotein particles of 20 nm diameter which are also secreted.

  7. Oncolytic virus therapies.

    Science.gov (United States)

    Buonaguro, Franco Maria; Tornesello, Maria Lina; Izzo, Francesco; Buonaguro, Luigi

    2012-11-01

    Oncolytic virus (OV) therapy currently represents one of the most promising approaches to cancer treatment for their dual anticancer mechanisms: direct lysis of cancer cells (oncolytic feature) and activation of the immunosystem (cancer vaccine aspect). The latter demonstrates the advantage of a multi-target approach against multiple tumor-associated antigens. Since the 2005 SFDA (the Chinese FDA) approval for the clinical use of Oncorine™, the first human OV-based cancer treatment, more than 200 patents have been filed worldwide and several Phase I/II studies have been conducted. This patent review analyzes patents and clinical studies of the most promising OV products to highlight the pros and cons of this innovative anticancer approach, which is currently being tested in several cancers (i.e., hepatocellular carcinoma, melanoma and glioblastoma) by systemic as well as intratumoral injection. Clinical results, although effective only for a limited period of time, are encouraging. Combined treatments with radio or chemotherapeutic protocols are also in progress. PMID:24236929

  8. A new ozone-based method for virus inactivation: preliminary study.

    Science.gov (United States)

    Kekez, M M; Sattar, S A

    1997-11-01

    The nebulization technique reported here could be used to inactivate viruses with ozone in large volumes of body fluids, such as plasma, partial blood and perhaps whole blood in a short time. Coliphage MS2 was used as a model because it is safe, easy to handle and more resistant to chemical disinfections than viruses such as HIV. The theoretical curves and experimental points, describing ozone inactivation of MS2, form a semi-sigmoid of congruent data. There was a > 7log10 reduction in MS2 viability and the possibilities of minimizing the ozone concentration required to kill viruses are indicated. The analysis was expanded to account for the interaction of ozone with a virus suspension in the shape of a thin film from the experimental findings of Bolton et al. We again find a semi-sigmoid of congruent data for their case, i.e. describing ozone inactivation of the influenza A virus (WSN strain) and the vesicular stomatitis virus versus time. For the method of nebulization, the exposure time of droplets with ozone is a few seconds, whereas for the thin film method the exposure time is measured in hours. PMID:9394395

  9. Immune based computer virus detection approaches

    Institute of Scientific and Technical Information of China (English)

    TAN Ying; ZHANG Pengtao

    2013-01-01

    The computer virus is considered one of the most horrifying threats to the security of computer systems worldwide.The rapid development of evasion techniques used in virus causes the signature based computer virus detection techniques to be ineffective.Many novel computer virus detection approaches have been proposed in the past to cope with the ineffectiveness,mainly classified into three categories:static,dynamic and heuristics techniques.As the natural similarities between the biological immune system (BIS),computer security system (CSS),and the artificial immune system (AIS) were all developed as a new prototype in the community of anti-virus research.The immune mechanisms in the BIS provide the opportunities to construct computer virus detection models that are robust and adaptive with the ability to detect unseen viruses.In this paper,a variety of classic computer virus detection approaches were introduced and reviewed based on the background knowledge of the computer virus history.Next,a variety of immune based computer virus detection approaches were also discussed in detail.Promising experimental results suggest that the immune based computer virus detection approaches were able to detect new variants and unseen viruses at lower false positive rates,which have paved a new way for the anti-virus research.

  10. Autophagic machinery activated by dengue virus enhances virus replication

    International Nuclear Information System (INIS)

    Autophagy is a cellular response against stresses which include the infection of viruses and bacteria. We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Autophagosome formation was also observed under the transmission electron microscope. DV2-induced autophagy further enhances the titers of extracellular and intracellular viruses indicating that autophagy can promote viral replication in the infected cells. Moreover, our data show that ATG5 protein is required to execute DV2-induced autophagy. All together, we are the first to demonstrate that DV can activate autophagic machinery that is favorable for viral replication

  11. [Bats and Viruses: complex relationships].

    Science.gov (United States)

    Rodhain, E

    2015-10-01

    With more than 1 200 species, bats and flying foxes (Order Chiroptera) constitute the most important and diverse order of Mammals after Rodents. Many species of bats are insectivorous while others are frugivorous and few of them are hematophagous. Some of these animals fly during the night, others are crepuscular or diurnal. Some fly long distances during seasonal migrations. Many species are colonial cave-dwelling, living in a rather small home range while others are relatively solitary. However, in spite of the importance of bats for terrestrial biotic communities and ecosystem ecology, the diversity in their biology and lifestyles remain poorly known and underappreciated. More than sixty viruses have been detected or isolated in bats; these animals are therefore involved in the natural cycles of many of them. This is the case, for instance, of rabies virus and other Lyssavirus (Family Rhabdoviridae), Nipah and Hendra viruses (Paramyxoviridae), Ebola and Marburg viruses (Filoviridae), SARS-CoV and MERS-CoV (Coronaviridae). For these zoonotic viruses, a number of bat species are considered as important reservoir hosts, efficient disseminators or even directly responsible of the transmission. Some of these bat-borne viruses cause highly pathogenic diseases while others are of potential significance for humans and domestic or wild animals; so, bats are an important risk in human and animal public health. Moreover, some groups of viruses developed through different phylogenetic mechanisms of coevolution between viruses and bats. The fact that most of these viral infections are asymptomatic in bats has been observed since a long time but the mechanisms of the viral persistence are not clearly understood. The various bioecology of the different bat populations allows exchange of virus between migrating and non-migrating conspecific species. For a better understanding of the role of bats in the circulation of these viral zoonoses, epidemiologists must pay attention to

  12. Coping with Computer Viruses: General Discussion and Review of Symantec Anti-Virus for the Macintosh.

    Science.gov (United States)

    Primich, Tracy

    1992-01-01

    Discusses computer viruses that attack the Macintosh and describes Symantec AntiVirus for Macintosh (SAM), a commercial program designed to detect and eliminate viruses; sample screen displays are included. SAM is recommended for use in library settings as well as two public domain virus protection programs. (four references) (MES)

  13. NMR study of xenotropic murine leukemia virus-related virus protease in a complex with amprenavir

    International Nuclear Information System (INIS)

    Highlights: ► Protease (PR) of XMR virus (XMRV) was successfully synthesized with cell-free system. ► Interface of XMRV PR with an inhibitor, amprenavir (APV), was identified with NMR. ► Structural heterogeneity is induced for two PR protomers in the APV:PR = 1:2 complex. ► Structural heterogeneity is transmitted even to distant regions from the interface. ► Long-range transmission of structural change may be utilized for drug discovery. -- Abstract: Xenotropic murine leukemia virus-related virus (XMRV) is a virus created through recombination of two murine leukemia proviruses under artificial conditions during the passage of human prostate cancer cells in athymic nude mice. The homodimeric protease (PR) of XMRV plays a critical role in the production of functional viral proteins and is a prerequisite for viral replication. We synthesized XMRV PR using the wheat germ cell-free expression system and carried out structural analysis of XMRV PR in a complex with an inhibitor, amprenavir (APV), by means of NMR. Five different combinatorially 15N-labeled samples were prepared and backbone resonance assignments were made by applying Otting’s method, with which the amino acid types of the [1H, 15N] HSQC resonances were automatically identified using the five samples (Wu et al., 2006) . A titration experiment involving APV revealed that one APV molecule binds to one XMRV PR dimer. For many residues, two distinct resonances were observed, which is thought to be due to the structural heterogeneity between the two protomers in the APV:XMRV PR = 1:2 complex. PR residues at the interface with APV have been identified on the basis of chemical shift perturbation and identification of the intermolecular NOEs by means of filtered NOE experiments. Interestingly, chemical shift heterogeneity between the two protomers of XMRV PR has been observed not only at the interface with APV but also in regions apart from the interface. This indicates that the structural heterogeneity

  14. Dot immunobinding assay for simultaneous detection of specific immunoglobulin G antibodies to measles virus, mumps virus, and rubella virus.

    OpenAIRE

    F. Condorelli; Ziegler, T.

    1993-01-01

    A dot immunobinding assay was used to detect antibodies to measles virus, mumps virus, and rubella virus antigens. Filter paper soaked with serum or whole blood was directly applied to the antigen-coated nitrocellulose sheets. The test was easy to perform, and its results agreed very well with those obtained by standard enzyme immunoassay.

  15. Droplet Microfluidics for Virus Discovery

    Science.gov (United States)

    Rotem, Assaf; Cockrell, Shelley; Guo, Mira; Pipas, James; Weitz, David

    2012-02-01

    The ability to detect, isolate, and characterize an infectious agent is important for diagnosing and curing infectious diseases. Detecting new viral diseases is a challenge because the number of virus particles is often low and/or localized to a small subset of cells. Even if a new virus is detected, it is difficult to isolate it from clinical or environmental samples where multiple viruses are present each with very different properties. Isolation is crucial for whole genome sequencing because reconstructing a genome from fragments of many different genomes is practically impossible. We present a Droplet Microfluidics platform that can detect, isolate and sequence single viral genomes from complex samples containing mixtures of many viruses. We use metagenomic information about the sample of mixed viruses to select a short genomic sequence whose genome we are interested in characterizing. We then encapsulate single virions from the same sample in picoliter volume droplets and screen for successful PCR amplification of the sequence of interest. The selected drops are pooled and their contents sequenced to reconstruct the genome of interest. This method provides a general tool for detecting, isolating and sequencing genetic elements in clinical and environmental samples.

  16. Scientists Try to Stop Another Deadly Virus

    Science.gov (United States)

    ... 158128.html Scientists Try to Stop Another Deadly Virus Junin, an Ebola-like disease in Argentina, has ... drug could offer a new weapon against the virus that causes Argentine hemorrhagic fever -- a potentially fatal ...

  17. A Literature Review of Zika Virus.

    Science.gov (United States)

    Plourde, Anna R; Bloch, Evan M

    2016-07-01

    Zika virus is a mosquitoborne flavivirus that is the focus of an ongoing pandemic and public health emergency. Previously limited to sporadic cases in Africa and Asia, the emergence of Zika virus in Brazil in 2015 heralded rapid spread throughout the Americas. Although most Zika virus infections are characterized by subclinical or mild influenza-like illness, severe manifestations have been described, including Guillain-Barre syndrome in adults and microcephaly in babies born to infected mothers. Neither an effective treatment nor a vaccine is available for Zika virus; therefore, the public health response primarily focuses on preventing infection, particularly in pregnant women. Despite growing knowledge about this virus, questions remain regarding the virus's vectors and reservoirs, pathogenesis, genetic diversity, and potential synergistic effects of co-infection with other circulating viruses. These questions highlight the need for research to optimize surveillance, patient management, and public health intervention in the current Zika virus epidemic. PMID:27070380

  18. Duck Virus Enteritis - A Contingency Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Duck plague, also known as duck virus enteritis (DVE) is a highly contagious, extremely deadly epizootic virus with a potential for devastating continental...

  19. Influenza Type A Viruses and Subtypes

    Science.gov (United States)

    ... Research Making a Candidate Vaccine Virus Related Links Influenza Types Seasonal Avian Swine Variant Pandemic Other Get ... this? Submit What's this? Submit Button Past Newsletters Influenza Type A Viruses Language: English Español Recommend ...

  20. About Epstein-Barr Virus (EBV)

    Science.gov (United States)

    ... Search The CDC Epstein-Barr Virus and Infectious Mononucleosis Note: Javascript is disabled or is not supported ... Mono Home About Epstein-Barr Virus About Infectious Mononucleosis For Healthcare Providers Laboratory Testing References & Resources About ...

  1. Bats and Viruses: a Brief Review

    Institute of Scientific and Technical Information of China (English)

    Lin-Fa Wang

    2009-01-01

    Bats, probably the most abundant, diverse and geographically dispersed vertebrates on earth, have recently been shown to be the reservoir hosts of a number of emerging viruses responsible for severe human and livestock disease outbreaks. Flying foxes have been demonstrated to be the natural reservoir for Hendra and Nipah viruses. Evidence supporting the possibility of bats as potential reservoirs for SARS coronavirus (SARS-CoV) and Ebola virus has also been reported. The recent discovery of these viruses and other viruses occurring naturally in the bat population provides a unique insight into a diverse pool of potentially emergent and pathogenic viruses. The factors which influence the ability of zoonotic viruses to effectively cross the species barrier from bats to other animal populations are poorly understood. A brief review is provided here on the recently emerged bat viruses and on current and future strategies for research in this area.

  2. West Nile Virus Cases, 2006-present

    Data.gov (United States)

    U.S. Department of Health & Human Services — This dataset contains positive cases of West Nile virus found in humans by county of residence, 2006-present. Humans usually become infected with West Nile virus by...

  3. FAQ: West Nile Virus and Dead Birds

    Science.gov (United States)

    ... Education Public Service Videos West Nile Virus & Dead Birds Recommend on Facebook Tweet Share Compartir On this ... dead bird sightings to local authorities. How do birds get infected with West Nile virus? West Nile ...

  4. Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)

    DEFF Research Database (Denmark)

    Kvisgaard, Lise Kirstine

    This PhD thesis presents the diversity of Porcine Reproductive and Respiratory Syndrome viruses (PRRSV) circulating in the Danish pig population. PRRS is a disease in pigs caused by the PRRS virus resulting in reproductive failures in sows and gilts and respiratory diseases in pigs . Due to genetic...... heterogeneity, PRRSV is divided into two genotypes, Type 1 and Type 2. Type 1 PRRS viruses are further divided into at least 3 subtypes. The virus evolves rapidly and reports of high pathogenic variants of both Type 1 and Type 2 appearing in Europe, North America, and Asia have been reported within recent years...... viruses showed both a higher diversity to the other Danish viruses and to the vaccine strain and one virus harbored the largest deletion in NSP2 reported in Danish Type 2 PRRSV. Manuscript IV is focusing on an experimental infection study in pigs with a Type 2 PRRS virus causing significant clinical...

  5. New Dengue Virus Vaccine Shows Promise

    Science.gov (United States)

    ... nih.gov/medlineplus/news/fullstory_157799.html New Dengue Virus Vaccine Shows Promise Research may also aid ... 16, 2016 (HealthDay News) -- An experimental vaccine against dengue -- the mosquito-borne virus behind a very painful ...

  6. Dengue Virus May Bolster Zika's Attack

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_159534.html Dengue Virus May Bolster Zika's Attack Prior exposure to ... 23, 2016 (HealthDay News) -- Prior exposure to the dengue fever virus may increase the severity of Zika ...

  7. Control of virus diseases of berry crops.

    Science.gov (United States)

    Martin, Robert R; Tzanetakis, Ioannis E

    2015-01-01

    Virus control in berry crops starts with the development of plants free of targeted pathogens, usually viruses, viroids, phytoplasmas, and systemic bacteria, through a combination of testing and therapy. These then become the top-tier plants in certification programs and are the source from which all certified plants are produced, usually after multiple cycles of propagation. In certification schemes, efforts are made to produce plants free of the targeted pathogens to provide plants of high health status to berry growers. This is achieved using a systems approach to manage virus vectors. Once planted in fruit production fields, virus control shifts to disease control where efforts are focused on controlling viruses or virus complexes that result in disease. In fruiting fields, infection with a virus that does not cause disease is of little concern to growers. Virus control is based on the use of resistance and tolerance, vector management, and isolation. PMID:25591882

  8. Nucleocytoplasmic Shuttling of Influenza A Virus Proteins

    Directory of Open Access Journals (Sweden)

    Jing Li

    2015-05-01

    Full Text Available Influenza viruses transcribe and replicate their genomes in the nuclei of infected host cells. The viral ribonucleoprotein (vRNP complex of influenza virus is the essential genetic unit of the virus. The viral proteins play important roles in multiple processes, including virus structural maintenance, mediating nucleocytoplasmic shuttling of the vRNP complex, virus particle assembly, and budding. Nucleocytoplasmic shuttling of viral proteins occurs throughout the entire virus life cycle. This review mainly focuses on matrix protein (M1, nucleoprotein (NP, nonstructural protein (NS1, and nuclear export protein (NEP, summarizing the mechanisms of their nucleocytoplasmic shuttling and the regulation of virus replication through their phosphorylation to further understand the regulation of nucleocytoplasmic shuttling in host adaptation of the viruses.

  9. General properties of grapevine viruses occurring in Hungary

    Directory of Open Access Journals (Sweden)

    Eszter Cseh

    2012-03-01

    Full Text Available The past fifty years important advances have been made in the field of grapevine virus research, including characterization of pathogens and control measurements. Still the occurrence of Grapevine fanleaf virus (GFLV, Arabis mosaic virus (ArMV, Tomato black ring virus (TBRV, Grapevine chrome mosaic virus (GCMV, Alfalfa mosaic virus (AMV, Grapevine Bulgarian latent virus (GBLV, Grapevine fleck virus (GFkV, Grapevine leafroll- associated viruses (GLRaV1-4, Grapevine virus A (GVA, Grapevine virus B (GVB and Grapevine rupestris stem pitting- associated virus (GRSPaV have been reported in Hungary and characterized by conventional methods as woody indexing, herbaceous indexing and serological methods. Among grapevine viruses the Grapevine line pattern virus (GLPV seems to be uncial; because it was reported only in Hungary. Causal agents of several grapevine diseases, like enation, vein necrosis and vein mosaic remained undiscovered. These virus-like diseases occurred only sporadically, without economic importance.

  10. Evolution of Computer Virus Concealment and Anti-Virus Techniques: A Short Survey

    CERN Document Server

    Rad, Babak Bashari; Ibrahim, Suhaimi

    2011-01-01

    This paper presents a general overview on evolution of concealment methods in computer viruses and defensive techniques employed by anti-virus products. In order to stay far from the anti-virus scanners, computer viruses gradually improve their codes to make them invisible. On the other hand, anti-virus technologies continually follow the virus tricks and methodologies to overcome their threats. In this process, anti-virus experts design and develop new methodologies to make them stronger, more and more, every day. The purpose of this paper is to review these methodologies and outline their strengths and weaknesses to encourage those are interested in more investigation on these areas.

  11. Exantema hemorrágico por virus dengue inducido por ácido acetil-salicílico Haemorrhagic exanthema due to dengue virus induced by acetylsalicylic acid

    OpenAIRE

    Valerio, L.; X. Balanzó; Jiménez, O.; M. L. Pedro-Botet

    2006-01-01

    El dengue, enfermedad infecciosa vírica propia de los climas tropicales, se considera una patología reemergente que ha dado lugar a graves epidemias en la última década. En la expansión del virus y de su mosquito vector se barajan factores relacionados con la alteración humana del medio, con la rapidez en el tránsito de mercancias y personas y debidos al cambio climático. Como reflejo de ello, se asiste a un aumento de casos importados que, al ser una enfermedad con periodo de incubación cort...

  12. Neuropatia enterica in un modello murino di infezione del sistema nervoso enterico con Herpes simplex virus di tipo 1

    OpenAIRE

    Qesari, Marsela

    2013-01-01

    Herpes simplex virus type 1 infection of murine enteric nervous system leads to gut neuropathy. The functional gastrointestinal disorders (FGIDs) are a heterogeneous group of chronic conditions characterized by disabling symptoms and decreased patients’ quality of life (Corazziari et al., 2004; Talley et al., 2008). Among these diseases, the Irritable Bowel Syndrome (IBS) is the most relevant for the severe prognosis and the high social and economic burden (Drossman, 2006; Fichna and Stor...

  13. Citrus tristeza virus-host interactions

    OpenAIRE

    Dawson, William O.; Garnsey, Stephen M.; Satyanarayana eTatineni; Folimonova, Svetlana Y.; Scott J Harper; S eGowda

    2013-01-01

    Citrus tristeza virus (CTV) is a phloem-limited virus whose natural host range is restricted to citrus and related species. Although the virus has killed millions of trees, almost destroying whole industries, and continually limits production in many citrus growing areas, most isolates are mild or symptomless in most of their host range. There is little understanding of how the virus causes severe disease in some citrus and none in others. Movement and distribution of CTV differs considerably...

  14. Inhibition of interferon signaling by dengue virus

    OpenAIRE

    Muñoz-Jordán, Jorge L.; Sánchez-Burgos, Gilma G.; Laurent-Rolle, Maudry; García-Sastre, Adolfo

    2003-01-01

    Dengue virus is a worldwide-distributed mosquito-borne flavivirus with a positive strand RNA genome. Its transcribed polyprotein is cleaved by host- and virus-encoded peptidases into 10 proteins, some of which are of unknown function. Although dengue virus-infected cells seem to be resistant to the antiviral action of IFN, the viral products that mediate this resistance are unknown. Therefore, we have analyzed the ability of the 10 dengue virus-encoded proteins to antagonize the IFN response....

  15. Viruses and prions of Saccharomyces cerevisiae

    OpenAIRE

    Wickner, Reed B.; Fujimura, Tsutomu; Esteban, Rosa

    2013-01-01

    Saccharomyces cerevisiae has been a key experimental organism for the study of infectious diseases, including dsRNA viruses, ssRNA viruses, and prions. Studies of the mechanisms of virus and prion replication, virus structure, and structure of the amyloid filaments that are the basis of yeast prions have been at the forefront of such studies in these classes of infectious entities. Yeast has been particularly useful in defining the interactions of the infectious elements with cellular compone...

  16. Viruses and Sialic Acids: Rules of Engagement

    OpenAIRE

    Neu, Ursula; Bauer, Johannes; Stehle, Thilo

    2011-01-01

    Viral infections are initiated by specific attachment of a virus particle to receptors at the surface of the host cell. For many viruses, these receptors are glycans that are linked to either a protein or a lipid. Glycans terminating in sialic acid and its derivatives serve as receptors for a large number of viruses, including several human pathogens. In combination with glycan array analyses, structural analyses of complexes of viruses with sialylated oligosaccharides have provided insights ...

  17. RNA virus genomics: a world of possibilities

    OpenAIRE

    Edward C Holmes

    2009-01-01

    The increasing availability of complete genome sequences of RNA viruses has the potential to shed new light on fundamental aspects of their biology. Here, I use case studies of 3 RNA viruses to explore the impact of genomic sequence data, with particular emphasis on influenza A virus. Notably, the studies of RNA virus genomics undertaken to date largely focused on issues of evolution and epidemiology, and they have given these disciplines new impetus. However, genomic data have so far made fe...

  18. Inhibition of Interferons by Ectromelia Virus

    OpenAIRE

    Smith, Vincent P.; Alcami, Antonio

    2002-01-01

    Ectromelia virus (EV) is an orthopoxvirus (OPV) that causes mousepox, a severe disease of laboratory mice. Mousepox is a useful model of OPV infection because EV is likely to be a natural mouse pathogen, unlike its close relatives vaccinia virus (VV) and variola virus. Several studies have highlighted the importance of mouse interferons (IFNs) in resistance to and recovery from EV infection, but little is known of the anti-IFN strategies encoded by the virus itself. We have determined that 12...

  19. Characteristics of Filoviridae: Marburg and Ebola Viruses

    Science.gov (United States)

    Beer, Brigitte; Kurth, Reinhard; Bukreyev, Alexander

    Filoviruses are enveloped, nonsegmented negative-stranded RNA viruses. The two species, Marburg and Ebola virus, are serologically, biochemically, and genetically distinct. Marburg virus was first isolated during an outbreak in Europe in 1967, and Ebola virus emerged in 1976 as the causative agent of two simultaneous outbreaks in southern Sudan and northern Zaire. Although the main route of infection is known to be person-to-person transmission by intimate contact, the natural reservoir for filoviruses still remains a mystery.

  20. The interferon sensitivity of selected porcine viruses.

    OpenAIRE

    Derbyshire, J. B.

    1989-01-01

    The objective of this study was to compare the sensitivity of 11 porcine viruses to the antiviral effects of porcine interferon-alpha in serum from piglets which had been infected 19 h previously with transmissible gastroenteritis virus, and of porcine interferon-beta prepared in PK-15 cells by induction with polyinosinic:polycytidylic acid, in yield reduction assays in pig kidney cells which were treated with interferon before virus challenge, and both before and after virus challenge. The m...

  1. Novel hemagglutinin-based influenza virus inhibitors

    OpenAIRE

    Shen, Xintian; Zhang, Xuanxuan; Liu, Shuwen

    2013-01-01

    Influenza virus has caused seasonal epidemics and worldwide pandemics, which caused tremendous loss of human lives and socioeconomics. Nowadays, only two classes of anti-influenza drugs, M2 ion channel inhibitors and neuraminidase inhibitors respectively, are used for prophylaxis and treatment of influenza virus infection. Unfortunately, influenza virus strains resistant to one or all of those drugs emerge frequently. Hemagglutinin (HA), the glycoprotein in influenza virus envelope, plays a c...

  2. Epistemologi Sufi : Perspektif Al-Hakim Al-Tirmidzi

    Directory of Open Access Journals (Sweden)

    Ainul Abidin Shah

    2012-06-01

    Full Text Available Abstract : This article deals with some epistemological terms and theoretical postulates in the thought of a well known sufi  from the 9th century AD., Abu `Abdu’lLah Muhammad b. `Ali al-Hakim al-Tirmidzi, in order to describe the path (sulūk of a sufi towards the Ultimate Reality. In his own style thought about the sufis, placing an emphasis upon the strong relations between the walāyah (sufi  sainthood and the ma`rifa (sufi knowledge in the one side, and between the two a philosophy of Sufi Ethics. So, it is nothing out of the ordinary to fi nd his ethical inclinations as a dominant feature in every aspect of his epistemological thoughts. In addition, sufi epistemology in the perspective of philosophers focuses on enforcing the inner positive potents of the Self more so than to eliminate “the satanic influences” to the heart, because – in al-Tirmidzi’s argument is that the former would greatly improve the immunity of the self, and encourage it to tread consistently on the right path as well.Keywords :  Dhawq, eros, heart, exoteric, esoteric, al-Haqq, al-`Adl, al-Shidq Abstrak : Artikel ini membahas istilah-istilah epistemologi dan beberapa postulat dari seorang sufi terkenal abad ke-9, Abu `Abdu’lLahMuh ammad b.`Ali al-Hakim al-Tirmidzi, dalam upaya menggambarkan perjalanan suluk seorang sufi menuju Realitas Mutlak. Dalam gaya pemikirannya tentang sufi, kita menemukan bahwa figur ini cenderung  menekankan hubungan-hubungan yang kuat antara wal āyah (kewalian sufi dan ma‘rifa (pengetahuan sufi di satu sisi, dan antara keduanya dengan filsafat moral sufi.  Jadi, tidak aneh jika kita menemukan kecenderungan - kecenderungan etis yang dominan dalam setiap aspek  pemikiran epistemologinya. Dan epistemologi sufi dalam perspektif para filsuf lebih menekankan potensi inner Diri yang positif daripada menghapuskan “cara-cara syaitan” dalam hati, karena - menurut pendapat al-Tirmidzi – ia akan meningkatkan

  3. Neutralisation and binding of VHS virus by monovalent antibody fragments

    DEFF Research Database (Denmark)

    Cupit, P.M.; Lorenzen, Niels; Strachan, G.; Kemp, G.J.L.; Secombes, C.J.; Cunningham, C.

    single chain antibody (scAb) fragments were generated from the three anti-VHSV Mabs and their variable domain genes, respectively. Fabs and scAbs derived from the neutralising Mabs were both able to neutralise the VHSV type 1 isolate DK-F1. In addition, a series of scAb fragments were produced using the......We have previously reported the cloning and characterisation of the heavy and light chain variable domain genes encoding three monoclonal antibodies (Mabs) that bind viral haemorrhagic septicaemia virus (VHSV). Two of these antibodies, 3F1H10 and 3F1A2 both neutralised the virus though 3F1A2...... appeared to recognise a broader range of virus isolates. The variable domains of these two antibodies differ by only four residues (Lorenzen et al., 2000a. Fish Shellfish Immunol. 10, 129-142). To further study the mechanism of neutralisation, Fab fragments as well as a series of recombinant bacterial...

  4. [Zika virus, an emerging threat].

    Science.gov (United States)

    Salinas, Sara; Foulongne, Vincent; Loustalot, Fabien; Fournier-Wirth, Chantal; Molès, Jean-Pierre; Briant, Laurence; Nagot, Nicolas; Van de Perre, Philippe; Simonin, Yannick

    2016-04-01

    Zika virus, discovered in 1947, is particularly publicized because of its involvement in a major epidemic that began in 2015 and which epicenter is located in Latin America, mainly in Brazil. In the majority of cases (70-80 %) the infection is asymptomatic, however in some patients, moderate fever, skin rash, conjunctivitis and myalgia may occur. More alarming, neurological complications are reported, in particular cases of microcephaly probably resulting from the infection of women in the first or second trimester of pregnancy. Moreover, Guillain-Barré syndromes have also been identified in patients whose infection was confirmed. The extent of the current outbreak reveals the very primitive state of knowledge about the pathophysiology of this virus. Thus, a global effort is being undertaken in order to quickly characterize the molecular interaction of the virus with human cells, but also to develop specific diagnostic assays and vaccinal approaches. PMID:27137695

  5. Virus Dynamics on Starlike Graphs

    CERN Document Server

    Becker, Thealexa; Kontorovich, Leonid Aryeh; Miller, Steven J; Ravikumar, Pradeep; Shen, Karen

    2011-01-01

    The field of epidemiology has presented fascinating and relevant questions for mathematicians, primarily concerning the spread of viruses in a community. The importance of this research has greatly increased over time as its applications have expanded to also include studies of electronic and social networks and the spread of information and ideas. We study virus propagation on a non-linear hub and spoke graph (which models well many airline networks). We determine the long-term behavior as a function of the cure and infection rates, as well as the number of spokes n. For each n we prove the existence of a critical threshold relating the two rates. Below this threshold, the virus always dies out; above this threshold, all non-trivial initial conditions iterate to a unique non-trivial steady state. We end with some generalizations to other networks.

  6. Hepatitis Virus Infections in Poultry.

    Science.gov (United States)

    Yugo, Danielle M; Hauck, Ruediger; Shivaprasad, H L; Meng, Xiang-Jin

    2016-09-01

    Viral hepatitis in poultry is a complex disease syndrome caused by several viruses belonging to different families including avian hepatitis E virus (HEV), duck hepatitis B virus (DHBV), duck hepatitis A virus (DHAV-1, -2, -3), duck hepatitis virus Types 2 and 3, fowl adenoviruses (FAdV), and turkey hepatitis virus (THV). While these hepatitis viruses share the same target organ, the liver, they each possess unique clinical and biological features. In this article, we aim to review the common and unique features of major poultry hepatitis viruses in an effort to identify the knowledge gaps and aid the prevention and control of poultry viral hepatitis. Avian HEV is an Orthohepevirus B in the family Hepeviridae that naturally infects chickens and consists of three distinct genotypes worldwide. Avian HEV is associated with hepatitis-splenomegaly syndrome or big liver and spleen disease in chickens, although the majority of the infected birds are subclinical. Avihepadnaviruses in the family of Hepadnaviridae have been isolated from ducks, snow geese, white storks, grey herons, cranes, and parrots. DHBV evolved with the host as a noncytopathic form without clinical signs and rarely progressed to chronicity. The outcome for DHBV infection varies by the host's ability to elicit an immune response and is dose and age dependent in ducks, thus mimicking the pathogenesis of human hepatitis B virus (HBV) infections and providing an excellent animal model for human HBV. DHAV is a picornavirus that causes a highly contagious virus infection in ducks with up to 100% flock mortality in ducklings under 6 wk of age, while older birds remain unaffected. The high morbidity and mortality has an economic impact on intensive duck production farming. Duck hepatitis virus Types 2 and 3 are astroviruses in the family of Astroviridae with similarity phylogenetically to turkey astroviruses, implicating the potential for cross-species infections between strains. Duck astrovirus (DAstV) causes

  7. Characterization of Ebola Virus Entry by Using Pseudotyped Viruses: Identification of Receptor-Deficient Cell Lines

    OpenAIRE

    Wool-Lewis, Rouven J.; Bates, Paul

    1998-01-01

    Studies analyzing Ebola virus replication have been severely hampered by the extreme pathogenicity of this virus. To permit analysis of the host range and function of the Ebola virus glycoprotein (Ebo-GP), we have developed a system for pseudotyping these glycoproteins into murine leukemia virus (MLV). This pseudotyped virus, MLV(Ebola), can be readily concentrated to titers which exceed 5 × 106 infectious units/ml and is effectively neutralized by antibodies specific for Ebo-GP. Analysis of ...

  8. Virus-Like Vesicle-Based Therapeutic Vaccine Vectors for Chronic Hepatitis B Virus Infection

    OpenAIRE

    Tracy D Reynolds; Buonocore, Linda; Rose, Nina F.; Rose, John K.; Robek, Michael D.

    2015-01-01

    More than 500,000 people die each year from the liver diseases that result from chronic hepatitis B virus (HBV) infection. Therapeutic vaccines, which aim to elicit an immune response capable of controlling the virus, offer a potential new treatment strategy for chronic hepatitis B. Recently, an evolved, high-titer vaccine platform consisting of Semliki Forest virus RNA replicons that express the vesicular stomatitis virus glycoprotein (VSV G) has been described. This platform generates virus...

  9. Antigen mimicry involving measles virus hemagglutinin and human respiratory syncytial virus nucleoprotein.

    OpenAIRE

    Norrby, E; Sheshberadaran, H; Rafner, B

    1986-01-01

    Intergenic antigenic relationships between measles virus and respiratory syncytial (RS) virus-specific structural components were studied by using monoclonal antibodies. Of 75 monoclonal antibodies against these components, only one, an anti-measles virus hemagglutinin monoclonal antibody, cross-reacted. Immunofluorescence analysis of measles virus- and RS virus-infected cells with this monoclonal antibody showed qualitatively different staining patterns which indicated that the antigen invol...

  10. Influenza A virus targets a cGAS-independent STING pathway that controls enveloped RNA viruses

    OpenAIRE

    Holm, Christian K.; Rahbek, Stine H.; Gad, Hans Henrik; Bak, Rasmus O.; Jakobsen, Martin R; Jiang, Zhaozaho; Hansen, Anne Louise; Jensen, Simon K.; Sun, Chenglong; Thomsen, Martin K.; Laustsen, Anders; Nielsen, Camilla G.; Severinsen, Kasper; Xiong, Yingluo; Burdette, Dara L.

    2016-01-01

    Stimulator of interferon genes (STING) is known be involved in control of DNA viruses but has an unexplored role in control of RNA viruses. During infection with DNA viruses STING is activated downstream of cGAMP synthase (cGAS) to induce type I interferon. Here we identify a STING-dependent, cGAS-independent pathway important for full interferon production and antiviral control of enveloped RNA viruses, including influenza A virus (IAV). Further, IAV interacts with STING through its conserve...

  11. More Effective Purifying Selection on RNA Viruses than in DNA Viruses

    OpenAIRE

    Hughes, Austin L.; Hughes, Mary Ann K.

    2007-01-01

    Analysis of the pattern of nucleotide diversity in 222 independent viral sequence datasets showed the prevalence of purifying selection. In spite of the higher mutation rate of RNA viruses, our analyses revealed stronger evidence of the action of purifying selection in RNA viruses than in DNA viruses. The ratio of nonsynonymous to synonymous nucleotide diversity was significantly lower in RNA viruses than in DNA viruses, indicating that nonsynonymous mutations have been removed at a greater r...

  12. Emerging viruses in the genus Comovirus

    Czech Academy of Sciences Publication Activity Database

    Petrzik, Karel; Koloniuk, Igor

    2010-01-01

    Roč. 40, č. 2 (2010), s. 290-292. ISSN 0920-8569 R&D Projects: GA ČR GA522/07/0053 Institutional research plan: CEZ:AV0Z50510513 Keywords : Capsid proteins * plant virus * Radish mosaic virus * Turnip ringspot virus Subject RIV: EE - Microbiology, Virology Impact factor: 1.693, year: 2010

  13. Emerging foodborne and agriculture-related viruses

    Science.gov (United States)

    Viruses rapidly evolve and can emerge in unpredictable ways. Mechanisms by which food-borne viruses may enter human populations and become pathogens is discussed. It is known the majority of viruses emerge from zoonotic animal reservoirs, often by adapting and infecting intermediate hosts, such as ...

  14. Ebola Virus Persistence in Semen Ex Vivo

    OpenAIRE

    Fischer, Robert J.; Judson, Seth; Miazgowicz, Kerri; Bushmaker, Trent; Munster, Vincent J.

    2016-01-01

    On March 20, 2015, a case of Ebola virus disease was identified in Liberia that most likely was transmitted through sexual contact. We assessed the efficiency of detecting Ebola virus in semen samples by molecular diagnostics and the stability of Ebola virus in ex vivo semen under simulated tropical conditions.

  15. Viruses infecting Brassicas in the Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Špak, Josef; Kubelková, Darina

    Bonn : DPG and BBA, 2002. s. 50. [Joint Conference of the International Working Groups on Legume Viruses and Vegetale Viruses /1./. 04.08.2002-09.08.2002, Bonn] Institutional research plan: CEZ:AV0Z5051902 Keywords : plant pathology * viruses Subject RIV: EE - Microbiology, Virology

  16. Virus infections of honeybees Apis Mellifera

    Directory of Open Access Journals (Sweden)

    Giuseppina Tantillo

    2015-09-01

    Full Text Available The health and vigour of honeybee colonies are threatened by numerous parasites (such as Varroa destructor and Nosema spp. and pathogens, including viruses, bacteria, protozoa. Among honeybee pathogens, viruses are one of the major threats to the health and wellbeing of honeybees and cause serious concern for researchers and beekeepers. To tone down the threats posed by these invasive organisms, a better understanding of bee viral infections will be of crucial importance in developing effective and environmentally benign disease control strategies. Here we summarize recent progress in the understanding of the morphology, genome organization, transmission, epidemiology and pathogenesis of eight honeybee viruses: Deformed wing virus (DWV and Kakugo virus (KV; Sacbrood virus (SBV; Black Queen cell virus (BQCV; Acute bee paralysis virus (ABPV; Kashmir bee virus (KBV; Israeli Acute Paralysis Virus (IAPV; Chronic bee paralysis virus (CBPV. The review has been designed to provide researchers in the field with updated information about honeybee viruses and to serve as a starting point for future research.

  17. El miedo al rey

    Directory of Open Access Journals (Sweden)

    François FORONDA

    2007-01-01

    Full Text Available La peur du roi. Sources et premières réflexions à propos d’une émotion aristocratique dans la Castille du XIVe siècleFace à un roi qui à présent pousse les effets de son ire jusqu’au meurtre pour faire le vide autour de lui et imposer sa différence souveraine, ses parents et les riches hommes, soit les principaux visés par cette politique de l’arbitraire qui enfreint les règles du jeu autant qu’elle contribue à les redéfinir, disent la peur (metus qu’ils ressentent, qu’ils utilisent aussi afin de donner à leur stratégie de pouvoir un fondement légitime. Et l’expression de cette émotion politique, qui tend à se muer en un stéréotype d’un discours aristocratique d’opposition, surtout présente dans les chroniques, mises ici en relation ici avec d’autres sources, notamment des correspondances, marque profondément l’histoire des relations souvent conflictuelles au sein de cette compagnie en définitive très étroite pendant un siècle, de la fin du XIIIe siècle à la fin du XIVe siècle. Cet article se propose d’ébaucher une première réflexion sur le sujet et d’en présenter les principales sources.El miedo al rey. Fuentes y primeras reflexiones acerca de una emoción aristocrática en la Castilla del siglo XIVFrente a un rey que ahora lleva hasta la muerte los efectos de su ira para hacer el vacío entorno a su persona e imponer su soberana, sus parientes y ricos hombres, o sea los principales afectados por esta política de la arbitrariedad que rompe las reglas del juego a la vez que contribuye a redefinirlas, dicen el miedo (metus que sienten, que utilizan también para darle a su estrategia de poder un fundamento legítimo. Y la expresión de esta emoción política, que tiende a convertirse en un estereotipo de un discurso aristocrático de oposición, sobre todo presente en la crónicas, aquí puestas en relación con otras fuentes, en particular cartas, marca profundamente la historia de

  18. Lagos bat virus in Kenya.

    Science.gov (United States)

    Kuzmin, Ivan V; Niezgoda, Michael; Franka, Richard; Agwanda, Bernard; Markotter, Wanda; Beagley, Janet C; Urazova, Olga Y; Breiman, Robert F; Rupprecht, Charles E

    2008-04-01

    During lyssavirus surveillance, 1,221 bats of at least 30 species were collected from 25 locations in Kenya. One isolate of Lagos bat virus (LBV) was obtained from a dead Eidolon helvum fruit bat. The virus was most similar phylogenetically to LBV isolates from Senegal (1985) and from France (imported from Togo or Egypt; 1999), sharing with these viruses 100% nucleoprotein identity and 99.8 to 100% glycoprotein identity. This genome conservancy across space and time suggests that LBV is well adapted to its natural host species and that populations of reservoir hosts in eastern and western Africa have sufficient interactions to share pathogens. High virus concentrations, in addition to being detected in the brain, were detected in the salivary glands and tongue and in an oral swab, suggesting that LBV is transmitted in the saliva. In other extraneural organs, the virus was generally associated with innervations and ganglia. The presence of infectious virus in the reproductive tract and in a vaginal swab implies an alternative opportunity for transmission. The isolate was pathogenic for laboratory mice by the intracerebral and intramuscular routes. Serologic screening demonstrated the presence of LBV-neutralizing antibodies in E. helvum and Rousettus aegyptiacus fruit bats. In different colonies the seroprevalence ranged from 40 to 67% and 29 to 46% for E. helvum and R. aegyptiacus, respectively. Nested reverse transcription-PCR did not reveal the presence of viral RNA in oral swabs of bats in the absence of brain infection. Several large bat roosts were identified in areas of dense human populations, raising public health concerns for the potential of lyssavirus infection. PMID:18305130

  19. Puesta en evidencia del virus diarrea viral bovina en bovinos clínicamente afectados Isolation of the bovine viral diarrhoea virus from tissue of clinically affected cattle

    Directory of Open Access Journals (Sweden)

    M.O CELEDON

    1997-01-01

    Full Text Available Para conocer la presencia del virus diarrea viral bovina (VDVB en animales sospechosos de estar cursando un cuadro clínico provocado por este virus, se trabajó con un total de 33 animales, correspondiendo a 23 fetos abortados, 2 mortinatos, un nonato, 3 vacas: una madre de mortinato, una madre de aborto y una muerta, 2 novillos muertos y 2 terneros muertos. Muestras de órganos se inocularon en cultivos primarios de pulmón fetal bovino (PFB y en la línea MDBK. Después del primer pasaje en células de PFB, se detectó la presencia de antígenos del VDVB por la prueba de inmunoperoxidasa indirecta (IPI. Todas las muestras con reacción positiva a IPI se inocularon por segunda y tercera vez en células de PFB, aplicándose la prueba de IPI en el tercer pasaje. Sobre un cuarto pasaje se aplicó la prueba de inmunofluorescencia direccta (IFD. Todas las muestras, positivas y negativas a IPI, se inocularon en 3 pasajes seriados en las células MDBK. En 23 de los 33 animales se aisló VDVB cepas no citopatogénicas (NCP, correspondiendo a 14 fetos abortados, un nonato, un mortinato, 3 vacas, 2 novillos y 2 terneros. En 6 fetos abortados, independiente de los infectados con el VDVB, se aisló el virus de la rinotraqueítis infecciosa bovina (RIB. Se concluye que la presencia del VDVB es de alta frecuencia en muestras clínicas de ganado bovino con patologías asociables al VDVB, desconociéndose el rol patógeno del virus en estos aislados.Cattle infected with the bovine viral diarrhoea (BVD virus can present a variety of clinical signs. This research studied the presence of BVD virus in cattle by virus isolation in primary cell cultures of bovine embryo lungs. Virus identification was done using the immunoperoxidase staining assay and the direct fluorescent antibody staining. As a result, 23 out of 33 animals were identified as positive to BVD virus: 14 foetal abortions, 2 stillbirths, 3 dams, 2 steers and 2 calves. No cytopathogenic isolates were

  20. Hepatitis C Virus Genotypes

    Directory of Open Access Journals (Sweden)

    Kayhan Azadmanesh

    2005-09-01

    Full Text Available IntroductionHepatitis C virus (HCV is an important cause of chronic liver disease. HCV causes 20% of acute hepatitis cases, 70% of all chronic hepatitis cases, 40% of all cases of liver cirrhosis, 60% of hepatocellular carcinomas, and 30% of liver transplants in Europe(1. It is also recognized as the leading cause of liver transplantation in the world(2. Only 20% of infected individuals will recover from this viral infection, while the rest become chronically infected(3. While the majority of chronically infected individuals never exhibit symptoms, approximately 10-30% of these patients will eventually develop cirrhosis or hepatocellular carcinoma, both of which are associated with significant morbidity and mortality(4.More than 170 million people worldwide are chronically infected with HCV. According to WHO report in 2002, chronic liver diseases were responsible for 1.4 million deaths, including 796,000 due to cirrhosis and 616,000 due to primary liver cancer. At least 20% of these deaths are probably attributable to HCV infection- more than 280,000 deaths(5, 6. The prevalence of chronic HCV infection in general population varies greatly in different parts of the world, being estimated between 0.1 and 5%, with a peak prevalence of 20- 25% in Egypt. HCV prevalence seems to be less than 1% in Iran, which is much lower than most of the neighboring countries(7. HCV was the first virus discovered by molecular cloning method without the direct use of biologic or biophysical methods. This was accomplished by extracting, copying into cDNA, and cloning all the nucleic acid from the plasma of a chimpanzee infected with non- A, non-B hepatitis by contaminated factor XIII concentrate(8. The HCV genome is a positive-sense, singlestranded RNA genome approximately 10 kb long. It has marked similarities to those of members of the genera Pestivirus and Flavivirus. Different HCV isolates from around the world show substantial nucleotide sequence variability

  1. Deletions within the 3' Non-Translated Region of Alfalfa mosaic virus RNA4 Do Not Affect Replication but Significantly Reduce Long-Distance Movement of Chimeric Tobacco mosaic virus

    Directory of Open Access Journals (Sweden)

    Vidadi Yusibov

    2013-07-01

    Full Text Available Alfalfa mosaic virus (AlMV RNAs 1 and 2 with deletions in their 3' non‑translated regions (NTRs have been previously shown to be encapsidated into virions by coat protein (CP expressed from RNA3, indicating that the 3' NTRs of RNAs 1 and 2 are not required for virion assembly. Here, we constructed various mutants by deleting sequences within the 3' NTR of AlMV subgenomic (sg RNA4 (same as of RNA3 and examined the effect of these deletions on replication and translation of chimeric Tobacco mosaic virus (TMV expressing AlMV sgRNA4 from the TMV CP sg promoter (Av/A4 in tobacco protoplasts and Nicotiana benthamiana plants. While the Av/A4 mutants were as competent as the wild-type Av/A4 in RNA replication in protoplasts, their encapsidation, long-distance movement and virus accumulation varied significantly in N. benthamiana. These data suggest that the 3' NTR of AlMV sgRNA4 contains potential elements necessary for virus encapsidation.

  2. Deletions within the 3' non-translated region of Alfalfa mosaic virus RNA4 do not affect replication but significantly reduce long-distance movement of chimeric Tobacco mosaic virus.

    Science.gov (United States)

    Roy, Gourgopal; Fedorkin, Oleg; Fujiki, Masaaki; Skarjinskaia, Marina; Knapp, Elisabeth; Rabindran, Shailaja; Yusibov, Vidadi

    2013-07-01

    Alfalfa mosaic virus (AlMV) RNAs 1 and 2 with deletions in their 3' non‑translated regions (NTRs) have been previously shown to be encapsidated into virions by coat protein (CP) expressed from RNA3, indicating that the 3' NTRs of RNAs 1 and 2 are not required for virion assembly. Here, we constructed various mutants by deleting sequences within the 3' NTR of AlMV subgenomic (sg) RNA4 (same as of RNA3) and examined the effect of these deletions on replication and translation of chimeric Tobacco mosaic virus (TMV) expressing AlMV sgRNA4 from the TMV CP sg promoter (Av/A4) in tobacco protoplasts and Nicotiana benthamiana plants. While the Av/A4 mutants were as competent as the wild-type Av/A4 in RNA replication in protoplasts, their encapsidation, long-distance movement and virus accumulation varied significantly in N. benthamiana. These data suggest that the 3' NTR of AlMV sgRNA4 contains potential elements necessary for virus encapsidation. PMID:23867804

  3. Activation analysis in virus research

    International Nuclear Information System (INIS)

    Nucleic acids contain various amounts of trace elements. With divalent cations tobacco mosaic virus ribonucleic acid (TMV-RNA) undergoes changes in optical density at 258 mμ in both optical rotation and in sedimentation behaviour. These changes suggested the transformation from a random coil to a more orderly configuration. The connection between trace elements and the biological activity of TMV-RNA has been investigated. However, the relationship between metal ions and the virus infection process has not received much attention. This subject is discussed briefly in this paper. 8 refs

  4. El pensamiento frente al arte

    OpenAIRE

    Bergmann, Antonio

    2012-01-01

    Es un hecho histórico de gran importancia el que el hombre haya creado arte antes de haberse dedicado a la filosofía. Al mencionar esto no pienso en las pinturas pre-históricas que se encuentran en las cuevas de España y en el Sur de Francia, sino ante todo, en el arte egipcio y mesopotámico.  En Egipto y Mesopotamia --y Mesopotamia parece ser la región más importante -no nació sino que se creó al hombre humano, al hombre como ser arquitectónico.

  5. The Current Outbreak of Ebola virus is still Continue in Guinea, Liberia and Sierra Leone: A Fact Sheet

    Directory of Open Access Journals (Sweden)

    Tauseef Ahmad

    2015-01-01

    Full Text Available The current outbreak of the Ebola virus occurred in the West Africa and it is the largest and more lethal outbreak in the history of Ebola virus. Thousands of peoples were infected along with thousands of death. The first case of current outbreak of Ebola virus was reported from Guine and acute;e Forestie` re (Forested Guinea, eastern area of Guinea in December 2013 (Ahmad, 2014; Baize et al., 2014; Gatherer, 2014. But in March 2014 the diseases spread so much fast and infected the surrounding countries Liberia and Sierra Leone. While in August 2014 the Ebola virus were reported from the Nigeria (Ahmad, 2014. [Biomed Res Ther 2015; 2(1.000: 193-195

  6. Detection of Influenza Virus with Specific Subtype by Using Localized Surface Plasmons Excited on a Flat Metal Surface

    Science.gov (United States)

    Ning, Jun; Nagata, Kotaro; Ainai, Akira; Hasegawa, Hideki; Kano, Hiroshi

    2013-08-01

    We report on a method to determine subtype of influenza viruses by using surface plasmons localized in microscopic region on a flat metal surface. In this method, refractive index variation arisen from interactions between viruses and their monoclonal antibodies is measured. The developed sensor shows stability of refractive index in the order of 10-4 against sample exchange. In our experiment, A/H1N1 viruses are distinguished from A/H3N2 viruses by using monoclonal antibodies immobilized on the metal surface. Since the measurement probe has the volume of ˜6 al, the method has potential to handle multiple subtypes in the measurement of a sample with ultra small volume.

  7. Strategies for Human Tumor Virus Discoveries: From Microscopic Observation to Digital Transcriptome Subtraction.

    Science.gov (United States)

    Mirvish, Ezra D; Shuda, Masahiro

    2016-01-01

    Over 20% of human cancers worldwide are associated with infectious agents, including viruses, bacteria, and parasites. Various methods have been used to identify human tumor viruses, including electron microscopic observations of viral particles, immunologic screening, cDNA library screening, nucleic acid hybridization, consensus PCR, viral DNA array chip, and representational difference analysis. With the Human Genome Project, a large amount of genetic information from humans and other organisms has accumulated over the last decade. Utilizing the available genetic databases, Feng et al. (2007) developed digital transcriptome subtraction (DTS), an in silico method to sequentially subtract human sequences from tissue or cellular transcriptome, and discovered Merkel cell polyomavirus (MCV) from Merkel cell carcinoma. Here, we review the background and methods underlying the human tumor virus discoveries and explain how DTS was developed and used for the discovery of MCV. PMID:27242703

  8. Beet mosaic virus: epidemiology and damage

    OpenAIRE

    Dusi, A.N.

    1999-01-01

    Overview:The aim of the studies described in this thesis was to obtain a thorough understanding of the main factors determining the spread of a potyvirus in a high plant density crop. The factors studied included the relationships between virus, host and vector, the spread of the virus around an initial virus source consisting of one or more infected plants, the spread of the virus by the prevailing aphid population, and the effect of plant density on the spread of the virus. A time-save samp...

  9. Radiation Inactivation of Viruses in Infected Products

    International Nuclear Information System (INIS)

    Full text: The effects of gamma radiation on foot-and-mouth disease virus in vitro and in situ have been studied. The data so far obtained show that a dose of 2 Mrad is required to inactivate virus in infected animal carcasses. But the dose may adversely affect the organoleptic quality of the meat. Experiments in vitro and in situ are necessary to study the effects of ionizing radiation on other viruses, such as rinderpest, swine fever and African swine fever-viruses, associated with animal products. Radiation may offer a possible means of eliminating the virus titre in many animal products and solve consequent quarantine problems. (author)

  10. Expanding networks of RNA virus evolution

    OpenAIRE

    Koonin Eugene V; Dolja Valerian V

    2012-01-01

    Abstract In a recent BMC Evolutionary Biology article, Huiquan Liu and colleagues report two new genomes of double-stranded RNA (dsRNA) viruses from fungi and use these as a springboard to perform an extensive phylogenomic analysis of dsRNA viruses. The results support the old scenario of polyphyletic origin of dsRNA viruses from different groups of positive-strand RNA viruses and additionally reveal extensive horizontal gene transfer between diverse viruses consistent with the network-like r...

  11. Viruses, dendritic cells and the lung

    Directory of Open Access Journals (Sweden)

    Graham Barney S

    2001-06-01

    Full Text Available Abstract The interaction between viruses and dendritic cells (DCs is varied and complex. DCs are key elements in the development of a host response to pathogens such as viruses, but viruses have developed survival tactics to either evade or diminish the immune system that functions to kill and eliminate these micro-organisms. In the present review we summarize current concepts regarding the function of DCs in the immune system, our understanding of how viruses alter DC function to attenuate both the virus-specific and global immune response, and how we may be able to exploit DC function to prevent or treat viral infections.

  12. A new looming of Zika virus

    Directory of Open Access Journals (Sweden)

    Nirav R. Soni

    2016-05-01

    Full Text Available Zika virus (ZIKV is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by daytime-active Aedes mosquitoes, such as A. aegypti. ZIKV will continue to spread and it will be difficult to determine how the virus will spread over time. Sign and symptoms of ZIKAVD (Zika virus disease were conjunctivitis (red eyes, back pain, birth defect-abnormal brain development known as microcephaly and it is diagnosed through PCR (polymerase chain reaction and virus isolation from blood samples.

  13. Consoer et al PFOS dataset

    Data.gov (United States)

    U.S. Environmental Protection Agency — This ScienceHub entry was developed for the published paper: Consoer et al., 2016, Toxicokinetics of perfluorooctane sulfonate in rainow trout (Oncorhynchus...

  14. DESAFÍOS AL ORDEN

    Directory of Open Access Journals (Sweden)

    Evangelina De los Rios

    2012-03-01

    Full Text Available Fradkin, Raúl y Gelman, Jorge (Compiladores, Desafíos al Orden. Política y sociedades rurales durante la Revolución de Independencia, Prohistoria ediciones, Rosario, 2008, 157 pp.

  15. Seltzer_et_al_2016

    Data.gov (United States)

    U.S. Environmental Protection Agency — This dataset supports the modeling study of Seltzer et al. (2016) published in Atmospheric Environment. In this study, techniques typically used for future air...

  16. Presence and distribution of economically important potato viruses in Montenegro

    OpenAIRE

    Jelena Zindović

    2011-01-01

    The research was carried out, in the period 2002-2004 in order to determine the presence and distribution of potato viruses at 12 different locations and on 9 different potato varieties grown in Montenegro. The research included collecting of samples in seed potato crops and testing of six economically important potato viruses: Potato leaf roll virus (PLRV), Potato virus Y (PVY), Potato virus X (PVX), Potato virus S (PVS), Potato virus A (PVA) i Potato viru...

  17. Oncogenes and RNA splicing of human tumor viruses

    OpenAIRE

    Ajiro, Masahiko; Zheng, Zhi-Ming

    2014-01-01

    Approximately 10.8% of human cancers are associated with infection by an oncogenic virus. These viruses include human papillomavirus (HPV), Epstein–Barr virus (EBV), Merkel cell polyomavirus (MCV), human T-cell leukemia virus 1 (HTLV-1), Kaposi's sarcoma-associated herpesvirus (KSHV), hepatitis C virus (HCV) and hepatitis B virus (HBV). These oncogenic viruses, with the exception of HCV, require the host RNA splicing machinery in order to exercise their oncogenic activities, a strategy that a...

  18. Identification of Potato Virus Y Strains in Tobacco Crops

    OpenAIRE

    Jelena Zindović; Janoš Berenji; Milena Pauković; Ivana Đekić; Aleksandra Bulajić; Branka Krstić

    2007-01-01

    Five viruses: Potato Virus Y (PVY), Tomato Spotted Wilt Virus, Cucumber Mosaic Virus, Tobacco Mosaic Virus and Alfalfa Mosaic Virus, of which PVY was predominant, were detected by serological testing of tobacco samples collected from many localities in Vojvodina in 2006. Viruses are the most important pathogens in tobacco and PVY causes considerable economic damages all over the world. A PVY population comprises several different strain groups, strain subgroups and recombinant strains. Among ...

  19. Note intorno al Teatro Vagante

    OpenAIRE

    Scabia, Giuliano

    2013-01-01

    In questo testo-intervista Scabia traccia, alternando racconto e riflessione, un percorso che abbracciatutta l'attività del suo Teatro Vagante: dalle azioni nelle scuole degli inizi degli anni '70 - agli alboridell'Animazione Teatrale, che anche da qui prese le mosse - al lavoro all'Università di Bologna,organica continuazione di quei primi esperimenti. Dalla poesia alle azioni teatrali in strada;dall'architettura della pagina al respiro della parola detta in presenza ; dalle aule ...

  20. Information als Gegenstand von Informationskompetenz

    OpenAIRE

    Ingold, Marianne

    2011-01-01

    Der Informationsbegriff als zentraler Gegenstand von Informationskompetenz wird in der bibliothekarischen Diskussion in der Regel nicht explizit thematisiert, sondern lässt sich aus Fachliteratur und Bibliothekspraxis nur implizit erschließen. Eine theoretische Beschäftigung mit dem Informationsbegriff ist jedoch unabdingbar, soll das Konzept "Informationskompetenz" auch außerhalb des bibliothekarischen Kontextes verständlich und nutzbar gemacht werden. Im vorliegenden Text, der sich als Beit...

  1. Response to Brosch et al.

    OpenAIRE

    Pihlajamäki, Jussi; Lerin, Carles; Kaminska, Dorota; Venesmaa, Sari; Itkonen, Paula; Boes, Tanner; Floss, Thomas; Schroeder, Joshua; Dearie, Farrell; Crunkhorn, Sarah; Burak, Furkan; Jimenez-Chillaron, Josep C.; Kuulasmaa, Tiina; Miettinen, Pekka; Park, Peter J.

    2012-01-01

    We would like to respond to Brosch et al. regarding our manuscript “Expression of the Splicing Factor Gene SFRS10 Is Reduced in Human Obesity and Contributes to Enhanced Lipogenesis” (Pihlajamäki et al., 2011b). Brosch performed RT-PCR in liver samples from 13 lean and 34 obese individuals, finding no differences in SFRS10 or LPIN1 expression. We wish to address points raised by Brosch, including experimental strategy and analysis of human SFRS10 expression.

  2. Sensitiveness of viruses to gamma radiation

    International Nuclear Information System (INIS)

    The sensitiveness of viruses to gamma rays was compared using eight viruses suspended with low concentration in drinking water, and four viruses present in high concentrations in tissue culture medium. The results show that the following factors are responsible for the resistance of viruses to gamma rays: 1. type of virus: the specific radiation resistance varied considerably; in general, there was a closer correlation with the general resistance of the virus to chemico-physical influences than with the type of nucleic acid of the virus examined; 2. medium of suspension and state of aggregation: high protein content and lyophilisation increased the resistance to gamma rays widely; 3. virus concentration: the virus reduction by a factor of 10 in suspensions with high virus concentration needed a higher radiation dose compared with suspensions of low virus content. All the results demonstrate the kinetics of inactivation to be a 1st order reaction. The increase of temperature to 410C did not show any significant influence. (orig.)

  3. Comparative analysis of chrysanthemum transcriptome in response to three RNA viruses: Cucumber mosaic virus, Tomato spotted wilt virus and Potato virus X.

    Science.gov (United States)

    Choi, Hoseong; Jo, Yeonhwa; Lian, Sen; Jo, Kyoung-Min; Chu, Hyosub; Yoon, Ju-Yeon; Choi, Seung-Kook; Kim, Kook-Hyung; Cho, Won Kyong

    2015-06-01

    The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV) and Potato virus X (PVX). A chrysanthemum 135K microarray derived from expressed sequence tags was successfully applied for the expression profiles of the chrysanthemum at early stage of virus infection. Finally, we identified a total of 125, 70 and 124 differentially expressed genes (DEGs) for CMV, TSWV and PVX, respectively. Many DEGs were virus specific; however, 33 DEGs were commonly regulated by three viruses. Gene ontology (GO) enrichment analysis identified a total of 132 GO terms, and of them, six GO terms related stress response and MCM complex were commonly identified for three viruses. Several genes functioning in stress response such as chitin response and ethylene mediated signaling pathway were up-regulated indicating their involvement in establishment of host immune system. In particular, TSWV infection significantly down-regulated genes related to DNA metabolic process including DNA replication, chromatin organization, histone modification and cytokinesis, and they are mostly targeted to nucleosome and MCM complex. Taken together, our comparative transcriptome analysis revealed several genes related to hormone mediated viral stress response and DNA modification. The identified chrysanthemums genes could be good candidates for further functional study associated with resistant to various plant viruses. PMID:25904110

  4. Emerging influenza virus: A global threat

    Indian Academy of Sciences (India)

    M Khanna; P Kumar; K Choudhary; B Kumar; V K Vijayan

    2008-11-01

    Since 1918, influenza virus has been one of the major causes of morbidity and mortality, especially among young children. Though the commonly circulating strain of the virus is not virulent enough to cause mortality, the ability of the virus genome to mutate at a very high rate may lead to the emergence of a highly virulent strain that may become the cause of the next pandemic. Apart from the influenza virus strain circulating in humans (H1N1 and H3N2), the avian influenza H5N1 H7 and H9 virus strains have also been reported to have caused human infections, H5N1 H7 and H9 have shown their ability to cross the species barrier from birds to humans and further replicate in humans. This review addresses the biological and epidemiological aspects of influenza virus and efforts to have a control on the virus globally.

  5. Expanding networks of RNA virus evolution

    Directory of Open Access Journals (Sweden)

    Koonin Eugene V

    2012-06-01

    Full Text Available Abstract In a recent BMC Evolutionary Biology article, Huiquan Liu and colleagues report two new genomes of double-stranded RNA (dsRNA viruses from fungi and use these as a springboard to perform an extensive phylogenomic analysis of dsRNA viruses. The results support the old scenario of polyphyletic origin of dsRNA viruses from different groups of positive-strand RNA viruses and additionally reveal extensive horizontal gene transfer between diverse viruses consistent with the network-like rather than tree-like mode of viral evolution. Together with the unexpected discoveries of the first putative archaeal RNA virus and a RNA-DNA virus hybrid, this work shows that RNA viral genomics has major surprises to deliver. See research article: http://www.biomedcentral.com/1471-2148/12/91

  6. Molecular patterns of avian influenza A viruses

    Institute of Scientific and Technical Information of China (English)

    KOU Zheng; LEI FuMin; WANG ShengYue; ZHOU YanHong; LI TianXian

    2008-01-01

    Avian influenza A viruses could get across the species barrier and be fatal to humans. Highly patho-genic avian influenza H5N1 virus was an example. The mechanism of interspecies transmission is not clear as yet. In this research, the protein sequences of 237 influenza A viruses with different subtypes were transformed into pseudo-signals. The energy features were extracted by the method of wavelet packet decomposition and used for virus classification by the method of hierarchical clustering. The clustering results showed that five patterns existed in avian influenza A viruses, which associated with the phenotype of interspecies transmission, and that avian viruses with patterns C and E could across species barrier and those with patterns A, B and D might not have the abilities. The results could be used to construct an early warning system to predict the transmissibility of avian influenza A viruses to humans.

  7. Molecular characterization of Indonesia avian influenza virus

    Directory of Open Access Journals (Sweden)

    N.L.P.I. Dharmayanti

    2005-06-01

    Full Text Available Avian influenza outbreaks in poultry have been reported in Java island since August 2003. A total of 14 isolates of avian influenza virus has been isolated from October 2003 to October 2004. The viruses have been identified as HPAI H5N1 subtype. All of them were characterized further at genetic level and also for their pathogenicity. Phylogenetic analysis showed all of the avian influenza virus isolates were closely related to avian influenza virus from China (A/Duck/China/E319-2/03(H5N1. Molecular basis of pathogenicity in HA cleavage site indicated that the isolates of avian influenza virus have multiple basic amino acid (B-X-B-R indicating that all of the isolates representing virulent avian influenza virus (highly pathogenic avian influenza virus.

  8. A Literature Review of Zika Virus

    Science.gov (United States)

    Bloch, Evan M.

    2016-01-01

    Zika virus is a mosquitoborne flavivirus that is the focus of an ongoing pandemic and public health emergency. Previously limited to sporadic cases in Africa and Asia, the emergence of Zika virus in Brazil in 2015 heralded rapid spread throughout the Americas. Although most Zika virus infections are characterized by subclinical or mild influenza-like illness, severe manifestations have been described, including Guillain-Barre syndrome in adults and microcephaly in babies born to infected mothers. Neither an effective treatment nor a vaccine is available for Zika virus; therefore, the public health response primarily focuses on preventing infection, particularly in pregnant women. Despite growing knowledge about this virus, questions remain regarding the virus’s vectors and reservoirs, pathogenesis, genetic diversity, and potential synergistic effects of co-infection with other circulating viruses. These questions highlight the need for research to optimize surveillance, patient management, and public health intervention in the current Zika virus epidemic. PMID:27070380

  9. Viruses and phytoplasmas infecting strawberry

    Czech Academy of Sciences Publication Activity Database

    Špak, Josef; Fránová, Jana

    Jokioinen : MTT Agrifood Research, 2008. s. 68. [COST863, WG2 and WG3 Joint SGM "Plant Health in Changing Environment . 19.05.2008-20.05.2008, Jokioinen] Institutional research plan: CEZ:AV0Z50510513 Keywords : plant disease * viruses * strawberry Subject RIV: EE - Microbiology, Virology

  10. Oncolytic viruses as anticancer vaccines

    Directory of Open Access Journals (Sweden)

    Norman eWoller

    2014-07-01

    Full Text Available Oncolytic virotherapy has shown impressive results in preclinical studies and first promising therapeutic outcomes in clinical trials as well. Since viruses are known for a long time as excellent vaccination agents, oncolytic viruses are now designed as novel anticancer agents combining the aspect of lysis-dependent cytoreductive activity with concomitant induction of antitumoral immune responses. Antitumoral immune activation by oncolytic virus infection of tumor tissue comprises both, immediate effects of innate immunity and also adaptive responses for long lasting antitumoral activity which is regarded as the most prominent challenge in clinical oncology. To date, the complex effects of a viral tumor infection on the tumor microenvironment and the consequences for the tumor-infiltrating immune cell compartment are poorly understood. However, there is more and more evidence that a tumor infection by an oncolytic virus opens up a number of options for further immunomodulating interventions such as systemic chemotherapy, generic immunostimulating strategies, dendritic cell-based vaccines, and antigenic libraries to further support clinical efficacy of oncolytic virotherapy.

  11. Bat Flight and Zoonotic Viruses

    Centers for Disease Control (CDC) Podcasts

    2014-05-30

    Reginald Tucker reads an abridged version of the EID perspective Bat Flight and Zoonotic Viruses.  Created: 5/30/2014 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 6/2/2014.

  12. Threat from Emerging Vectorborne Viruses

    Centers for Disease Control (CDC) Podcasts

    2016-06-09

    Reginald Tucker reads an abridged version of the commentary by CDC author Ronald Rosenberg, Threat from Emerging Vectorborne Viruses.  Created: 6/9/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 6/9/2016.

  13. Control of feline leukaemia virus.

    NARCIS (Netherlands)

    K. Weijer (Kees); F.G.C.M. Uytdehaag (Fons); A.D.M.E. Osterhaus (Ab)

    1989-01-01

    textabstractFeline leukaemia virus (FeLV) usually occurs in its natural species, the domestic cat. FeLV is also important to human individuals as a comparative model, as it may cause a variety of diseases, some malignant and some benign, such as immunosuppression, which bears a resemblance to AIDS (

  14. Enumerating viruses in coral mucus

    OpenAIRE

    Leruste, Amandine; Bouvier, Thierry; Bettarel, Yvan

    2012-01-01

    The distribution of viruses inhabiting the coral mucus remains undetermined, as there is no suitable standardized procedure for their separation from this organic matrix, principally owing to its viscosity and autofluorescence. Seven protocols were tested, and the most efficient separations were obtained from a chemical treatment requiring potassium citrate.

  15. Enumerating viruses in coral mucus.

    Science.gov (United States)

    Leruste, Amandine; Bouvier, Thierry; Bettarel, Yvan

    2012-09-01

    The distribution of viruses inhabiting the coral mucus remains undetermined, as there is no suitable standardized procedure for their separation from this organic matrix, principally owing to its viscosity and autofluorescence. Seven protocols were tested, and the most efficient separations were obtained from a chemical treatment requiring potassium citrate. PMID:22729548

  16. Molecular phylogeny of Duvenhage virus

    Directory of Open Access Journals (Sweden)

    Louis H. Nel

    2011-11-01

    Full Text Available The Duvenhage virus (DUVV constitutes one of the 11 species in the Lyssavirus genus and causes fatal rabies encephalitis. The virus is associated with insectivorous bat species and three human cases have been reported, all of which were linked to contact with bats. Few of these isolates have been studied and thus little is known about the phylogeny and epidemiology of this lyssavirus. Until 2007, when an isolate was made from the East African country of Kenya, all isolations of this virus had been from southern Africa. This discovery led to many questions regarding the spread and diversity of this lyssavirus. Phylogenetic analysis indicated that the DUVV isolates constitute two different lineages, in which the southern African isolates group together to form one lineage and the more recent isolate from Kenya constitutes a new, second lineage. We found that the new isolate has a genetic variation that has not yet been seen for DUVV. Not only is our lack of knowledge regarding the geographical distribution of this uniquely African virus emphasised, but we have also demonstrated the potential diversity within this genotype.

  17. Sandfly Fever Sicilian Virus, Algeria

    OpenAIRE

    Izri, Arezki; Temmam, Sarah; Moureau, Grégory; Hamrioui, Boussad; de Lamballerie, Xavier; Charrel, Rémi N.

    2008-01-01

    To determine whether sandfly fever Sicilian virus (SFSV) is present in Algeria, we tested sandflies for phlebovirus RNA. A sequence closely related to that of SFSV was detected in a Phlebotomus ariasi sandfly. Of 60 human serum samples, 3 contained immunoglobulin G against SFSV. These data suggest SFSV is present in Algeria.

  18. Mayaro Fever Virus, Brazilian Amazon

    OpenAIRE

    Azevedo, Raimunda S. S.; Silva, Eliana V. P.; Carvalho, Valéria L.; Rodrigues, Sueli G.; Neto, Joaquim P. Nunes; Monteiro, Hamilton A. de O.; Peixoto, Victor S.; Chiang, Jannifer O.; Nunes, Márcio R. T.; Vasconcelos, Pedro F.C.

    2009-01-01

    In February 2008, a Mayaro fever virus (MAYV) outbreak occurred in a settlement in Santa Barbara municipality, northern Brazil. Patients had rash, fever, and severe arthralgia lasting up to 7 days. Immunoglobulin M against MAYV was detected by ELISA in 36 persons; 3 MAYV isolates sequenced were characterized as genotype D.

  19. Mutants of alfalfa mosaic virus

    International Nuclear Information System (INIS)

    In this thesis the isolation and characterization of a number of mutants of alfalfa mosaic virus, a plant virus with a coat protein dependent genome, is described. Thermo-sensitive (ts) mutants were selected since, at least theoretically, ts mutations can be present in all virus coded functions. It was found that a high percentage of spontaneous mutants, isolated because of their aberrant symptoms, were ts. The majority of these isolates could grow at the non-permissive temperature in the presence of a single wild type (wt) component. To increase the mutation rate virus preparations were treated with several mutagens. After nitrous acid treatment or irradiation with ultraviolet light, an increase in the level of mutations was observed. UV irradiation was preferred since it did not require large amounts of purified viral components. During the preliminary characterization of potential ts mutants the author also obtained one structural and several symptom mutants which were analysed further (chapter 7, 8 and 9). The properties of the ts mutants are described in chapter 3-7. (Auth.)

  20. West Nile Virus Neuroinvasive Disease

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2009-07-01

    Full Text Available Epidemiological features of West Nile Virus (WNV disease among children (<18 years of age reported to the Centers for Disease Control and Prevention from 1999 through 2007 were analyzed and compared with those of adult WNV neuroinvasive disease (WNND, in a study at CDC&P, Fort Collins, CO.

  1. A Case of Ebola Virus

    Centers for Disease Control (CDC) Podcasts

    2012-10-01

    Dr. Adam MacNeil, an epidemiologist at CDC, discusses Ebola virus.  Created: 10/1/2012 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID); National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 10/1/2012.

  2. Microcephaly and the Zika virus.

    Science.gov (United States)

    2016-09-12

    Cases of microcephaly brought about by the Zika virus have brought professional and personal challenges for nurses in Brazil. Paediatric nurses, such as Roberta Seabra (pictured), take over as part of the multidisciplinary team once the baby is born. In this article health writer Jacqui Thornton presents some personal stories. PMID:27615590

  3. Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells

    International Nuclear Information System (INIS)

    Psoralen compounds covalently bind to nucleic acids when irradiated with long-wavelength ultraviolet light. This treatment can destroy the infectivity of deoxyribonucleic acid and ribonucleic acid viruses. Two psoralen compounds, 4'-hydroxymethyltrioxsalen and 4'-aminomethyltrioxsalen, were used with long-wavelength ultraviolet light to inactivate cell-free herpes simplex and influenza viruses and to render virus-infected cells noninfectious. This method of inactivation was compared with germicidal (short-wavelength) ultraviolet light irradiation. The antigenicity of the treated, virus-infected, antigen-bearing cells was examined by immunofluorescence and radioimmunoassay and by measuring the capacity of the herpes simplex virus-infected cells to stimulate virus-specific lymphocyte proliferation. The infectivity of the virus-infected cells could be totally eliminated without altering their viral antigenicity. The use of psoralen plus long-wavelength ultraviolet light is well suited to the preparation of noninfectious virus antigens and virus antigen-bearing cells for immunological assays

  4. Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates.

    Science.gov (United States)

    Stanković, I; Bulajić, A; Vučurović, A; Ristić, D; Milojević, K; Berenji, J; Krstić, B

    2011-01-01

    In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively. TSWV was the most frequently found virus at the localities of Central Serbia, while PVY and CMV were the most frequent viruses in the Vojvodina Province. Single infections were prevalent in years 2005-2007 and the most frequent were those of PVY. A triple combination of those viruses was most frequent mixed infection type in 2008. The presence of all five detected viruses was confirmed in selected ELISA-positive samples by RT-PCR and sequencing. The comparisons of obtained virus isolate sequences with those available in NCBI, confirmed the authenticity of serologically detected viruses. Phylogenetic analysis based on partial nucleocapsid gene sequences revealed a joint clustering of Serbian, Bulgarian and Montenegrin TSWV isolates into one geographic subpopulation, which was distinct from the other subpopulation of TSWV isolates from the rest of the European countries. The high incidence of viruses in Serbian tobacco crops highlights the importance of enhancing farmers knowledge towards better implementation of control strategies for preventing serious losses. PMID:22149499

  5. DNA microarray global gene expression analysis of influenza virus-infected chicken and duck cells

    Directory of Open Access Journals (Sweden)

    Suresh V. Kuchipudi

    2015-06-01

    Full Text Available The data described in this article pertain to the article by Kuchipudi et al. (2014 titled “Highly Pathogenic Avian Influenza Virus Infection in Chickens But Not Ducks Is Associated with Elevated Host Immune and Pro-inflammatory Responses” [1]. While infection of chickens with highly pathogenic avian influenza (HPAI H5N1 virus subtypes often leads to 100% mortality within 1 to 2 days, infection of ducks in contrast causes mild or no clinical signs. The rapid onset of fatal disease in chickens, but with no evidence of severe clinical symptoms in ducks, suggests underlying differences in their innate immune mechanisms. We used Chicken Genechip microarrays (Affymetrix to analyse the gene expression profiles of primary chicken and duck lung cells infected with a low pathogenic avian influenza (LPAI H2N3 virus and two HPAI H5N1 virus subtypes to understand the molecular basis of host susceptibility and resistance in chickens and ducks. Here, we described the experimental design, quality control and analysis that were performed on the data set. The data are publicly available through the Gene Expression Omnibus (GEOdatabase with accession number GSE33389, and the analysis and interpretation of these data are included in Kuchipudi et al. (2014 [1].

  6. Miocarditis fulminante y enfermedad diarreica aguda por Coxsackie virus B6 Fulminant myocarditis and acute gastroenteritis due to Coxsackie virus B6

    Directory of Open Access Journals (Sweden)

    Germán Málaga

    2011-03-01

    Full Text Available Presentamos el caso de una paciente joven que presentó choque cardiogénico por virus Coxsakie B6. La paciente acudió a una clínica particular con un cuadro clínico compatible con gastroenterocolitis aguda a la que después de una hora de estar recibiendo hidratación y manejo del cuadro diagnosticado, se agregó hipotensión que llegó al estado de choque, hipoxemia severa y compromiso pulmonar bilateral intersticial por lo que ingresó a Unidad de Cuidados Intensivos, donde recibió manejo de soporte. Debido al cuadro clínico y elevación de enzimas cardiacas se sospechó de compromiso cardiaco, la ecocardiografía evidenció cambios sugerentes de miocarditis. La evolución fue favorable y se le pudo dar de alta después de una semana. El diagnóstico etiológico del cuadro se hizo en el seguimiento, presentando serología con elevación de títulos para virus Coxsakie B6.We present the case of a young woman who suffered cardiogenic due to by Coxsackie virus B6. The patient attended a private clinic with an acute gastroenteritis and after one hour of receiving hydratation,she developed hypotension and shock, severe hypoxemia and bilateral lung infiltrate. The patient entered the Intensive Care Unit, where she received hemodynamic support. Due to the clinical picture and cardiac enzymes increase, a cardiac failure was suspected and the echocardiographic findings suggested "myocarditis". The evolution was successful and Coxsackie B6 virus infection diagnosis was made during the follow up by increase of the levels of antibodies for virus Coxsackie B6.

  7. D’al-Ẓāhir Baybars à ʿAlī al-Zaybaq

    OpenAIRE

    Dorlian, Georges

    2014-01-01

    « Toi par le courage, moi par la filouterie » aimait répéter Baṭṭāl à la princesse Ḏāt al-Himma, comme pour délimiter les espaces d’action revenant à chacun. Et d’ajouter, pour mieux dépeindre son rôle : « Mon métier n’est pas celui de la guerre, des armes et des exploits, mais celui de la ruse et de la fourberie dans un château fort ou une forteresse. » Baṭṭāl était un ʿayyār adjoint à la princesse Ḏāt al-Himma et à ʿAbd al-Wahhāb pour servir leur cause sublime et leur idéal chevaleresque. E...

  8. Hepatitis Virus Infection and Hodgkin's Lymphoma: A Review of the Literature

    Directory of Open Access Journals (Sweden)

    Viroj Wiwanitkit

    2007-11-01

    Full Text Available IntroductionKeresztes et al. showed that numerous observations implied that the pathogenesis of malignant lymphomas was multifactorial and that viruses probably played an important etiologic role (1. Lymphoma is a common hematological malignancy. Fisher and colleague said that several pathogens have been linked to the risk of lymphoma, including Epstein-Barr virus, human immunodeficiency virus, hepatitis virus, and simian virus 40 (2. Hepatitis viruses especially for hepatitis C (3, 4 and hepatitis G (3 have been mentioned as a risk factor for development of Hodgkin's lymphoma. In this work, the author summarized the evidence on the correlation between Hodgkin's lymphoma and hepatitis virus infection focusing on hepatitis C and hepatitis G.Hepatitis C Infection and Hodgkin's LymphomaThere are many studies on the carcinogenesis role of hepatitis C virus (HCV. Major topics of the study are the biological functions of HCV proteins and the interaction between HCV proteins and cellular proteins (5. HCV infection has shown to be strongly linked to the development of hepatocellular carcinoma (HCC in epidemiological studies (6. Unlike other human oncogenic viruses, HCV is a typical RNA virus, and thus there is no integration of the viral genome or a piece of the genome into host chromosomes (6. Moreover, trans-acting transcriptional factors, which are coded by other human oncogenic viruses and required primarily for virus replication and often involved in cell immortalization, may not be coded by HCV (6.Hausfater et al. mentioned that the finding of HCV binding on CD81, a surface-expressed protein present on lymphocyte membrane, enhanced the putative role of HCV in lymphoma genesis (7. Observations made with isolated HCV antigens and/or with HCV subgenomic replicon systems demonstrated that the products encoded in the HCV genome interfere with and disturb intracellular signal transduction, often by phosphorylation of cellular proteins (8. Moreover, some

  9. Clinical and biological differences between recurrent herpes simplex virus and varicella-zoster virus infections

    International Nuclear Information System (INIS)

    The major features that distinguish recurrent herpes simplex virus infections from zoster are illustrated in this article by two case histories. The clinical and epidemiologic features that characterize recurrent herpes simplex virus and varicella-zoster virus infections are reviewed. It is noted that herpesvirus infections are more common and severe in patients with cellular immune deficiency. Each virus evokes both humoral and cellular immune response in the course of primary infection. DNA hybridization studies with RNA probes labelled with sulfur-35 indicate that herpes simplex viruses persist within neurons, and that varicella-zoster virus is found in the satellite cells that encircle the neurons

  10. Contemporary North American influenza H7 viruses possess human receptor specificity: Implications for virus transmissibility

    DEFF Research Database (Denmark)

    Belser, Jessica A; Blixt, Ola; Chen, Li-Mei; Pappas, Claudia; Maines, Taronna R; Van Hoeven, Neal; Donis, Ruben; Busch, Julia; McBride, Ryan; Paulson, James C; Katz, Jacqueline M; Tumpey, Terrence M

    2008-01-01

    viruses from The Netherlands in 2003 maintained the classic avian-binding preference for alpha2-3-linked sialic acids (SA) and are not readily transmissible in ferrets, as observed previously for highly pathogenic H5N1 viruses. However, H7N3 viruses isolated from Canada in 2004 and H7N2 viruses from the...... in the upper respiratory tract of ferrets and was capable of transmission in this species by direct contact. These results indicate that H7 influenza viruses from the North American lineage have acquired sialic acid-binding properties that more closely resemble those of human influenza viruses and...

  11. Strain sensing with sub-micron sized Al-AlOx-Al tunnel junctions

    OpenAIRE

    Koppinen, P. J.; Lievonen, J. T.; Ahlskog, M.; Maasilta, I. J.

    2009-01-01

    We demonstrate a local strain sensing method for nanostructures based on metallic Al tunnel junctions with AlOx barriers. The junctions were fabricated on top of a thin silicon nitride membrane, which was actuated with an AFM tip attached to a stiff cantilever. A large relative change in the tunneling resistance in response to the applied strain (gauge factor) was observed, up to a value 37. This facilitates local static strain variation measurements down to ~10^{-7}.

  12. Gloves and Ebola virus:a consideration of the penetration possibility based on nanostructure consideration

    Institute of Scientific and Technical Information of China (English)

    Sora; Yasri; Viroj; Wiwanitkit

    2015-01-01

    <正>To the editor,Ebola virus infection is the new deadly viral infection that is presently problematic in many countries in Africa.The prevention of this disease seems to be difficult and the rapid distribution of disease becomes the important issue for further discussion.As noted by Muyembe-Tamfum et al.[1],one of the major disease control measures is the distribution of protective equipment to health care workers.Gloves are important protective equipments

  13. Small non-coding RNAs, mammalian cells, and viruses: Regulatory interactions?

    OpenAIRE

    Benkirane Monsef; Jeang Kuan-Teh; Yeung Man

    2007-01-01

    Recent findings suggest that mammalian cells can use small non-coding RNAs (ncRNA) to regulate physiological viral infections. Here, we comment on several lines of evidence that support this concept. We discuss how viruses may in turn protect, suppress, evade, modulate, or adapt to the host cell's ncRNA regulatory schema. © 2007 Yeung et al; licensee BioMed Central Ltd.

  14. PCNA Interacts with Indian Mung Bean Yellow Mosaic Virus Rep and Downregulates Rep Activity

    OpenAIRE

    Bagewadi, Basavaraj; Chen, Shoajiang; Sunil K. Lal; Choudhury, Nirupam Roy; Mukherjee, Sunil K

    2004-01-01

    Proliferative cell nuclear antigen (PCNA), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. The biochemical role of PCNA in rolling circle replication (RCR) of geminivirus DNA has not been explored in detail. The initiation of RCR of the bipartite genome of a geminivirus, Indian mung bean yellow mosaic virus (IMYMV), is mainly controlled by viral protein Rep (or AL1 or AC1). The ro...

  15. Linfomas asociados con la infección por el virus de la inmunodeficiencia humana: subtipos histológicos y asociación con los virus de Epstein Barr y Herpes-8 AIDS related lymphomas: Histopathological subtypes and association with Epstein Barr virus and Human Herpes virus type-8

    OpenAIRE

    Marcelo Corti; Marcela de Dios Soler; Patricia Bare; María F Villafañe; Miguel de Tezanos Pinto; Raúl Pérez Bianco; Marina Narbaitz

    2010-01-01

    Los linfomas no Hodgkin (LNH) constituyen la segunda neoplasia definitoria de Sida más frecuente. En el presente trabajo se evaluaron 48 casos de linfomas asociados con la enfermedad debida al virus de la inmunodeficiencia humana (HIV) diagnosticados en la División Histopatología del Instituto de Investigaciones Hematológicas de la Academia Nacional de Medicina. Se incluyeron en la investigación 5 mujeres y 43 hombres con una mediana de edad al momento del diagnóstico de la neoplasia de 37 añ...

  16. Epistemologi Sufi : Perspektif Al-Hakim Al-Tirmidzi

    OpenAIRE

    Ainul Abidin Shah

    2012-01-01

    Abstract : This article deals with some epistemological terms and theoretical postulates in the thought of a well known sufi  from the 9th century AD., Abu `Abdu’lLah Muhammad b. `Ali al-Hakim al-Tirmidzi, in order to describe the path (sulūk) of a sufi towards the Ultimate Reality. In his own style thought about the sufis, placing an emphasis upon the strong relations between the walāyah (sufi  sainthood) and the ma`rifa (sufi knowledge) in the one side, and between the two a philosophy of Sufi Et...

  17. 21 CFR 866.3305 - Herpes simplex virus serological assays.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Herpes simplex virus serological assays. 866.3305... simplex virus serological assays. (a) Identification. Herpes simplex virus serological assays are devices... herpes simplex virus in serum. Additionally, some of the assays consist of herpes simplex virus...

  18. Blueberry latent virus: An Amalgam of the Totiviridae and Partitiviridae

    Science.gov (United States)

    A new, symptomless virus was identified in blueberry. The dsRNA genome of the virus, provisionally named Blueberry latent virus (BBLV), codes for two putative proteins and lacks a movement protein, a property only shared with cryptic viruses. More than 35 isolates of the virus from different cultiv...

  19. 9 CFR 113.206 - Wart Vaccine, Killed Virus.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Wart Vaccine, Killed Virus. 113.206... AGRICULTURE VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS STANDARD REQUIREMENTS Killed Virus Vaccines § 113.206 Wart Vaccine, Killed Virus. Wart Vaccine, Killed Virus, shall be...

  20. 9 CFR 113.311 - Bovine Virus Diarrhea Vaccine.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Bovine Virus Diarrhea Vaccine. 113.311... Virus Vaccines § 113.311 Bovine Virus Diarrhea Vaccine. Bovine Virus Diarrhea Vaccine shall be prepared... virus diarrhea susceptible calves shall be used as test animals (20 vaccinates and five controls)....