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Sample records for al gen env

  1. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002027 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002027 ABOL01019614 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + His GTG ...

  2. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001609 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001609 ABNJ01088362 mosquito metageno...me; viral fraction from mixed species mosquitoes collected at Buena Vista Lagoon in Oceanside, CA + Met CAT ...

  3. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001574 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001574 ABNJ01021258 mosquito metageno...me; viral fraction from mixed species mosquitoes collected at Buena Vista Lagoon in Oceanside, CA - Leu TAA ...

  4. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002049 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002049 ABOL01033247 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Leu TAA ...

  5. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002157 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002157 ABOL01346421 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Ala TGC ...

  6. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002045 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002045 ABOL01029424 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - His GTG ...

  7. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002132 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002132 ABOL01258260 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Lys TTT ...

  8. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002099 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002099 ABOL01173632 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Asp GTC ...

  9. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002048 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002048 ABOL01030883 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + His GTG ...

  10. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001591 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001591 ABNJ01052532 mosquito metageno...me; viral fraction from mixed species mosquitoes collected at Buena Vista Lagoon in Oceanside, CA - Pro TGG ...

  11. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001608 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001608 ABNJ01079185 mosquito metageno...me; viral fraction from mixed species mosquitoes collected at Buena Vista Lagoon in Oceanside, CA - Tyr GTA ...

  12. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001624 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001624 ABNJ01130619 mosquito metageno...me; viral fraction from mixed species mosquitoes collected at Buena Vista Lagoon in Oceanside, CA - Met CAT ...

  13. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002095 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002095 ABOL01163528 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Ile GAT ...

  14. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002100 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002100 ABOL01173734 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Ile GAT ...

  15. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002186 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002186 ABOL01582464 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + His GTG ...

  16. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001572 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001572 ABNJ01018487 mosquito metageno...me; viral fraction from mixed species mosquitoes collected at Buena Vista Lagoon in Oceanside, CA + Tyr GTA ...

  17. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002064 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002064 ABOL01058741 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Arg CCT ...

  18. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001573 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001573 ABNJ01020371 mosquito metageno...me; viral fraction from mixed species mosquitoes collected at Buena Vista Lagoon in Oceanside, CA + Leu CAA ...

  19. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002046 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002046 ABOL01030261 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - His GTG ...

  20. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002063 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002063 ABOL01055660 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - His GTG ...

  1. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002131 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002131 ABOL01253080 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Ile GAT ...

  2. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002130 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002130 ABOL01252839 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Glu TTC ...

  3. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002171 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002171 ABOL01418533 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Ala GGC ...

  4. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002115 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002115 ABOL01202194 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Ile GAT ...

  5. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001610 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001610 ABNJ01089745 mosquito metageno...me; viral fraction from mixed species mosquitoes collected at Buena Vista Lagoon in Oceanside, CA + Lys TTT ...

  6. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002062 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002062 ABOL01054589 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - His GTG ...

  7. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002023 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002023 ABOL01018038 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - His GTG ...

  8. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002117 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002117 ABOL01206716 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Leu CAA ...

  9. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002098 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002098 ABOL01173441 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Gly CCC ...

  10. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001627 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001627 ABNJ01138625 mosquito metageno...me; viral fraction from mixed species mosquitoes collected at Buena Vista Lagoon in Oceanside, CA - Asn GTT ...

  11. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002028 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002028 ABOL01020878 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + His GTG ...

  12. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002113 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002113 ABOL01199944 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Ile GAT ...

  13. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002139 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002139 ABOL01268880 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Ile GAT ...

  14. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002114 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002114 ABOL01201499 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Ile GAT ...

  15. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002047 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002047 ABOL01030601 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - His GTG ...

  16. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001625 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001625 ABNJ01132810 mosquito metageno...me; viral fraction from mixed species mosquitoes collected at Buena Vista Lagoon in Oceanside, CA - Lys TTT ...

  17. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002172 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002172 ABOL01426301 mosquito metageno...me; viral fraction from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Glu TTC ...

  18. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001702 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001702 ABNY01028851 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + His GTG ...

  19. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001708 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001708 ABNY01042488 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Gly TCC ...

  20. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001716 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001716 ABNY01090914 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Gly GCC ...

  1. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001687 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001687 ABNY01010710 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Phe GAA ...

  2. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001694 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001694 ABNY01022656 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Val TAC ...

  3. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001699 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001699 ABNY01027832 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Tyr GTA ...

  4. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001704 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001704 ABNY01034507 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Met CAT ...

  5. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001706 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001706 ABNY01038212 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Ala TGC ...

  6. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001679 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001679 ABNY01001243 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Trp CCA ...

  7. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001712 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001712 ABNY01068626 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Arg ACG ...

  8. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001686 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001686 ABNY01010544 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Leu TAG ...

  9. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001680 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001680 ABNY01002362 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Trp CCA ...

  10. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001709 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001709 ABNY01042852 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Gln TTG ...

  11. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001683 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001683 ABNY01007198 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Met CAT ...

  12. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001681 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001681 ABNY01004105 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Leu TAG ...

  13. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001698 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001698 ABNY01026275 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + His GTG ...

  14. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001685 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001685 ABNY01008451 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Asp GTC ...

  15. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001713 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001713 ABNY01068850 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Cys GCA ...

  16. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001697 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001697 ABNY01025533 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Ser TGA ...

  17. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001701 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001701 ABNY01028675 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + His GTG ...

  18. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001691 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001691 ABNY01015907 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Ser GGA ...

  19. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001695 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001695 ABNY01023764 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Leu TAG ...

  20. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001689 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001689 ABNY01011527 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Gln TTG ...

  1. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001682 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001682 ABNY01006742 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Met CAT ...

  2. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001714 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001714 ABNY01072206 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Asn GTT ...

  3. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001693 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001693 ABNY01021289 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Leu TAG ...

  4. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001710 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001710 ABNY01043563 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Ala TGC ...

  5. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001707 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001707 ABNY01038603 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Gly TCC ...

  6. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001700 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001700 ABNY01028239 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Leu GAG ...

  7. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001684 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001684 ABNY01007726 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA - Gln TTG ...

  8. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001711 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001711 ABNY01068122 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Asn GTT ...

  9. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001696 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001696 ABNY01024252 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Leu TAG ...

  10. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001715 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001715 ABNY01081602 saltern metagenome; microbial... fraction from plasmids from marine microbial community in low salinity saltern in San Diego, CA + Arg CCG ...

  11. Factores genéticos de pacientes con ictus asociados al pronóstico

    OpenAIRE

    Giralt Casellas, Dolors

    2016-01-01

    El objetivo de este trabajo es, utilizando una base de datos de pacientes que han padecido un ictus, encontrar tendencias entre pronóstico al alta y medicamentos que toman los pacientes previamente al ictus. Seguidamente, cruzar los factores genéticos asociados anteriormente con bases de datos del genoma humano para ver los genes asociados a las tendencias encontradas y ver la drogabilidad de los genes. L'objectiu d'aquest treball és, utilitzant una base de dades de pacients que han patit ...

  12. ENV 100 UOP course tutorial/tutorialoutlet

    OpenAIRE

    NARESH 1

    2015-01-01

    For more course tutorials visit www.tutorialoutlet.com   ENV 100 Week 1 DQ 1   ENV 100 Week 1 DQ 2   ENV 100 Week 1 DQ 3   ENV 100 Week 1 Assignment Environmental Fundamentals Paper   ENV 100 Week 2 DQ 1   ENV 100 Week 2 DQ 2   ENV 100 Week 2 DQ 3   ENV 100 Week 2 Learning Team Assignment Ecosystems Paper   ENV 100 Week 3 DQ 1   ENV 100 Week 3 DQ 2   ...

  13. Estudio de polimorfismos genéticos y respuesta al tratamiento en pacientes con migraña cronica

    OpenAIRE

    Navarro Ortega, Nicolás

    2015-01-01

    Se han llevado a cabo diversos estudios de asociación genómicos tipo GWAS (Genome Wide Association Studies) utilizando polimorfismos de un solo nucleótido (SNP) con el objeto de identificar regiones genómicas que estuvieran relacionadas tanto con la migraña, como con los tratamientos y los efectos de los mismos, así como el uso de polimorfismos que han sido publicados por su relación con la migraña (Anttila et al., 2010; Chasman et al., 2011; De Vries et al., 2009; Freilinger et al., 2012; Li...

  14. Optimized Gen-II FeCrAl cladding production in large quantity for campaign testing

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, Yukinori [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Sun, Zhiqian [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Pint, Bruce A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Terrani, Kurt A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2016-06-03

    There are two major objectives in this report; (1) to optimize microstructure control of ATF FeCrAl alloys during tube drawing processes, and (2) to provide an update on the progress of ATF FeCrAl tube production via commercial manufacturers. Experimental efforts have been made to optimize the process parameters balancing the tube fabricability, especially for tube drawing processes, and microstructure control of the final tube products. Lab-scale sheet materials of Gen II FeCrAl alloys (Mo-containing and Nb-containing FeCrAl alloys) were used in the study, combined with a stepwise warm-rolling process and intermediate annealing, aiming to simulate the tube drawing process in a commercial tube manufacturer. The intermediate annealing at 650ºC for 1h was suggested for the tube-drawing process of Mo-containing FeCrAl alloys because it successfully softened the material by recovering the work hardening introduced through the rolling step, without inducing grain coarsening due to recrystallization. The final tube product is expected to have stabilized deformed microstructure providing the improved tensile properties with sufficient ductility. Optimization efforts on Nb-containing FeCrAl alloys focused on the effect of alloying additions and annealing conditions on the stability of deformed microstructure. Relationships between the second-phase precipitates (Fe2Nb-Laves phase) and microstructure stability are discussed. FeCrAl tube production through commercial tube manufacturers is currently in progress. Three different manufacturers, Century Tubes, Inc. (CTI), Rhenium Alloys, Inc. (RAI), and Superior Tube Company, Inc. (STC), are providing capabilities for cold-drawing, warm-drawing, and HPTR cold-pilgering, respectively. The first two companies are currently working on large quantity tube production (expected 250 ft length) of Gen I model FeCrAl alloy (B136Y3, at CTI) and Gen II (C35M4, at RAI), with the process parameters obtained from the experimental

  15. ENV 322 ASH Material-env322dotcom

    OpenAIRE

    Sandywilliam1

    2015-01-01

    ENV 322 Entire Course (Ash) For more course tutorials visit www.env322.com ENV 322 Complete Class Business - General Business   Gaia Hypothesis. In Chapter 3, the Gaia hypothesis is introduced. It addresses two hypotheses; (1) life, since its inception, has greatly affected the planetary environment and (2) life has altered Earth’s environment in ways that have allowed life to persist. Do you agree with these two hypotheses? Why or why not? Energy an...

  16. Env 322 ASH Course Tutorial / env322dotcom

    OpenAIRE

    anil14

    2015-01-01

    ENV 322 Entire Course (Ash) For more course tutorials visit www.env322.com ENV 322 Complete Class Business - General Business   Gaia Hypothesis. In Chapter 3, the Gaia hypothesis is introduced. It addresses two hypotheses; (1) life, since its inception, has greatly affected the planetary environment and (2) life has altered Earth’s environment in ways that have allowed life to persist. Do you agree with these two hypotheses? Why or why not? Energy an...

  17. ALV-J GP37 molecular analysis reveals novel virus-adapted sites and three tyrosine-based Env species.

    Science.gov (United States)

    Ye, Jianqiang; Fan, Zhonglei; Shang, Jianjun; Tian, Xiaoyan; Yang, Jialiang; Chen, Hongjun; Shao, Hongxia; Qin, Aijian

    2015-01-01

    Compared to other avian leukosis viruses (ALV), ALV-J primarily induces myeloid leukemia and hemangioma and causes significant economic loss for the poultry industry. The ALV-J Env protein is hypothesized to be related to its unique pathogenesis. However, the molecular determinants of Env for ALV-J pathogenesis are unclear. In this study, we compared and analyzed GP37 of ALV-J Env and the EAV-HP sequence, which has high homology to that of ALV-J Env. Phylogenetic analysis revealed five groups of ALV-J GP37 and two novel ALV-J Envs with endemic GP85 and EAV-HP-like GP37. Furthermore, at least 15 virus-adapted mutations were detected in GP37 compared to the EAV-HP sequence. Further analysis demonstrated that three tyrosine-based motifs (YxxM, ITIM (immune tyrosine-based inhibitory motif) and ITAM-like (immune tyrosine-based active motif like)) associated with immune disease and oncogenesis were found in the cytoplasmic tail of GP37. Based on the potential function and distribution of these motifs in GP37, ALV-J Env was grouped into three species, inhibitory Env, bifunctional Env and active Env. Accordingly, 36.91%, 61.74% and 1.34% of ALV-J Env sequences from GenBank are classified as inhibitory, bifunctional and active Env, respectively. Additionally, the Env of the ALV-J prototype strain, HPRS-103, and 17 of 18 EAV-HP sequences belong to the inhibitory Env. And models for signal transduction of the three ALV-J Env species were predicted. Our findings and models provide novel insights for identifying the roles and molecular mechanism of ALV-J Env in the unique pathogenesis of ALV-J. PMID:25849207

  18. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update

    OpenAIRE

    Peakall, Rod; Smouse, Peter E.

    2012-01-01

    Summary: GenAlEx: Genetic Analysis in Excel is a cross-platform package for population genetic analyses that runs within Microsoft Excel. GenAlEx offers analysis of diploid codominant, haploid and binary genetic loci and DNA sequences. Both frequency-based (F-statistics, heterozygosity, HWE, population assignment, relatedness) and distance-based (AMOVA, PCoA, Mantel tests, multivariate spatial autocorrelation) analyses are provided. New features include calculation of new estimators of popula...

  19. Cabells blancs, un destí també escrit als gens

    OpenAIRE

    Bueno i Torrens, David, 1965-

    2016-01-01

    Un dels aspectes més evidents del pas del temps és l'emblanquiment dels cabells. Tanmateix, aquest procés únicament es produeix en les persones d'ascendència europea. En les d'origen asiàtic, africà i amerindi, és un fet rar i excepcional. Andrés Ruiz-Linares i els seus col·laboradors, de diverses universitats i centres de recerca europeus, sud-americans i australians, han aïllat el gen que el provoca, i també han identificat disset gens més que contribueixen a definir les característiques ge...

  20. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001973 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001973 ABOK01143647 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Met CAT ...

  1. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001933 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001933 ABOK01048051 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Gly GCC ...

  2. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001990 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001990 ABOK01217516 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Val GAC ...

  3. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001991 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001991 ABOK01218729 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Lys TTT ...

  4. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001917 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001917 ABOK01014385 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Met CAT ...

  5. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002006 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002006 ABOK01337352 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Val GAC ...

  6. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001919 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001919 ABOK01017442 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Gln CTG ...

  7. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001960 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001960 ABOK01092952 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Cys GCA ...

  8. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001975 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001975 ABOK01148089 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Val TAC ...

  9. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001932 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001932 ABOK01046212 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Asp GTC ...

  10. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001959 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001959 ABOK01092151 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Ile GAT ...

  11. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001916 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001916 ABOK01013921 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Gln TTG ...

  12. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08002005 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08002005 ABOK01308326 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Val GAC ...

  13. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001934 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001934 ABOK01050102 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Arg CCT ...

  14. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001918 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001918 ABOK01017030 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Arg CCT ...

  15. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001958 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001958 ABOK01090996 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Lys TTT ...

  16. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001939 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001939 ABOK01055049 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA + Cys GCA ...

  17. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001974 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001974 ABOK01144549 mosquito metageno...me; viral fraction from Mung bean nuclease digestion of DNA from mixed species mosquitoes collected in Mission Valley in San Diego, CA - Leu TAG ...

  18. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001677 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001677 ABNV01056443 coral metagenome; microbial fr ... (Ca(NO3)2), nitrite (NaNO2), ammonium (NH4Cl), and phosphate ... (KH2PO4) that was changed every 24 hours; collecte ...

  19. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001671 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001671 ABNP01030221 coral metagenome; viral fracti ... (Ca(NO3)2), nitrite (NaNO2), ammonium (NH4Cl), and phosphate ... (KH2PO4) that was changed every 24 hours; collecte ...

  20. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001670 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001670 ABNP01013218 coral metagenome; viral fracti ... (Ca(NO3)2), nitrite (NaNO2), ammonium (NH4Cl), and phosphate ... (KH2PO4) that was changed every 24 hours; collecte ...

  1. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001669 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001669 ABNP01005558 coral metagenome; viral fracti ... (Ca(NO3)2), nitrite (NaNO2), ammonium (NH4Cl), and phosphate ... (KH2PO4) that was changed every 24 hours; collecte ...

  2. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001674 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001674 ABNV01009958 coral metagenome; microbial fr ... (Ca(NO3)2), nitrite (NaNO2), ammonium (NH4Cl), and phosphate ... (KH2PO4) that was changed every 24 hours; collecte ...

  3. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001675 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001675 ABNV01026676 coral metagenome; microbial fr ... (Ca(NO3)2), nitrite (NaNO2), ammonium (NH4Cl), and phosphate ... (KH2PO4) that was changed every 24 hours; collecte ...

  4. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001676 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001676 ABNV01034095 coral metagenome; microbial fr ... (Ca(NO3)2), nitrite (NaNO2), ammonium (NH4Cl), and phosphate ... (KH2PO4) that was changed every 24 hours; collecte ...

  5. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001666 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001666 ABNO01002212 coral metagenome; viral fracti ... riment that consisted of 5 liters of seawater with molecular ... grade HCl added to a final pH 68 that was changed ...

  6. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001668 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001668 ABNO01021040 coral metagenome; viral fracti ... riment that consisted of 5 liters of seawater with molecular ... grade HCl added to a final pH 68 that was changed ...

  7. tRNA配列、アノテーション及びキュレーションのデータ: >ENV08001667 [tRNADB-CE (tRNA gene database curated manually by experts)

    Lifescience Database Archive (English)

    Full Text Available Environmental sample (ENV) from GenBank >ENV08001667 ABNO01019954 coral metagenome; viral fracti ... riment that consisted of 5 liters of seawater with molecular ... grade HCl added to a final pH 68 that was changed ...

  8. Incorporation of chimeric HIV-SIV-Env and modified HIV-Env proteins into HIV pseudovirions

    International Nuclear Information System (INIS)

    Low level incorporation of the viral glycoprotein (Env) into human immunodeficiency virus (HIV) particles is a major drawback for vaccine strategies against HIV/AIDS in which HIV particles are used as immunogen. Within this study, we have examined two strategies aimed at achieving higher levels of Env incorporation into non-infectious pseudovirions (PVs). First, we have generated chimeric HIV/SIV Env proteins containing the truncated C-terminal tail region of simian immunodeficiency virus (SIV)mac239-Env767stop, which mediates strongly increased incorporation of SIV-Env into SIV particles. In a second strategy, we have employed a truncated HIV-Env protein (Env-Tr752N750K) which we have previously demonstrated to be incorporated into HIV virions, generated in infected T-cells, to a higher level than that of Wt-HIV-Env. Although the chimeric HIV/SIV Env proteins were expressed at the cell surface and induced increased levels of cell-cell fusion in comparison to Wt-HIV-Env, they did not exhibit increased incorporation into either HIV-PVs or SIV-PVs. Only Env-Tr752N750K exhibited significantly higher (threefold) levels of incorporation into HIV-PVs, an improvement, which, although not dramatic, is worthwhile for the large-scale preparation of non-infectious PVs for vaccine studies aimed at inducing Env humoral responses

  9. Mutationen des Androgenrezeptor-Gens als mögliche Ursache der Antiandrogenresistenz beim Prostatakarzinom

    Directory of Open Access Journals (Sweden)

    Röpke A

    2004-01-01

    Full Text Available Der Androgenrezeptor (AR ist ein nukleärer Transkriptionsfaktor, der die intrazelluläre Wirkung der Androgene vermittelt. Das Prostatakarzinom ist in Deutschland das häufigste Krebsleiden beim Mann. Bei fortgeschrittenem Prostatakarzinom ist eine radikale Entfernung der Prostata nicht immer möglich. In diesen Fällen wird auf eine palliative endokrine Behandlung zurückgegriffen. Da primäre Prostatakarzinome in ihrem Wachstum meist androgenabhängig sind, ist die Ansprechrate auf eine Antiandrogentherapie entsprechend hoch und liegt bei ca. 75 %. Mit der Antiandrogentherapie wird versucht, die Konzentration von freizirkulierenden Androgenen herabzusetzen oder die transkriptionelle Eigenschaft des AR zu blockieren. Bei den meisten Patienten kommt es jedoch trotz einer zunächst erfolgreichen Antiandrogentherapie sekundär unter der Therapie zu einer erneuten Proliferation oder Metastasierung des Prostatakarzinoms. Bei ca. 25 % der Patienten wird bereits primär eine Resistenz gegenüber der Antiandrogentherapie beobachtet. Für das Versagen der Antiandrogentherapie können Veränderungen des AR im Tumor verantwortlich sein. Bei Prostatakarzinomen mit sekundärer Antiandrogenresistenz besteht häufig eine Amplifikation des AR-Gens. Dagegen zeigen Prostatakarzinome ohne Antiandrogentherapie sehr selten (1 % eine Amplifikation des AR-Genlokus, jedoch in 11 % eine Polysomie des X-Chromosoms, einschließlich des AR-Gens. Mutationen im AR-Gen werden bei metastasierten oder hormonrefraktären, seltener bei primären Prostatakarzinomen beschrieben. Es konnte in einer Vielzahl von Studien festgestellt werden, daß verschiedene Steroidhormone eine höhere Affinität zu mutierten AR aufweisen, dadurch zu einer höheren transkriptionellen Aktivität führen und so die zelluläre Proliferation stimulieren.

  10. El comentario de textos literarios en Secundaria: del modelo genérico al intertextual

    OpenAIRE

    Caro Valverde, María Teresa; González García, María

    2009-01-01

    El documento presenta un modelo de aprendizaje significativo del comentario de textos, tanto desde el tradicional punto de vista genérico como desde el innovador punto de vista intertextual.Por medio de guías de comentario para ambos casos, seguidas de ejemplos comentados y de una selección de páginas Web escogidas, el lector dispone de una visión estructural, pragmática y heurística sobre este tema didáctico.

  11. Microsatélites amplificados al azar (RAM en estudios de diversidad genética vegetal

    Directory of Open Access Journals (Sweden)

    Jaime Eduardo Muñoz Flórez

    2008-12-01

    Full Text Available Se revisó el uso e importancia, ventajas, desventajas y características de la técnica Microsatélites Amplificados al Azar (RAM en uchuva Physalis peruviana, mora Rubus spp, guayaba Psidium guajava y heliconias Heliconia spp. En mora se diferenciaron las especies R. glaucus, R. robustus y R. urticifolius, se detectaron duplicados y se encontró alta variabilidad genética en R. glaucus, la especie más importante. En uchuva se encontró alta diversidad y dos accesiones de fruto rojo que se diferenciaron genéticamente de las amarillas y una región geográfica con alta variabilidad. En guayaba los cebadores fueron altamente polimórficos y se encontró alta variabilidad en el Valle del Cauca. En heliconias y especies relacionadas se diferenciaron las familias del orden Zingiberales, algunos subgéneros y variaciones en la especie. La técnica es de bajo costo, utiliza un cebador, no requiere información previa, es altamente polimórfica y diferencia especies en los taxones evaluados.

  12. Intervista al GEN. B. Mariano MOSSA, Comandante Carabinieri Tutela Patrimonio Culturale

    Directory of Open Access Journals (Sweden)

    Luca Papi

    2014-09-01

    Full Text Available In the framework of the cultural heritage, several records are recognized to our Country. With regard to them, it is worth to mention the establishment of a selected body of investigators Carabinieri Tutela Patrimonio Culturale specifically dedicated to combating crimes against cultural heritage.The Interview to Gen. B. Mariano Mossa is mainly focused to highlight the experience gained over more than 40 years of activity by the mentioned body. In particular, it will be illustrated the features and services which can enjoy a citizen by downloading the mobile app developed by Carabinieri Tutela Patrimonio Culturale.

  13. Microsatélites amplificados al azar (RAM en estudios de diversidad genética vegetal

    Directory of Open Access Journals (Sweden)

    Muñoz Flórez Jaime Eduardo

    2008-12-01

    Full Text Available Se revisó el uso e importancia, ventajas, desventajas y características de la técnica Microsatélites Amplificados al Azar (RAM en uchuva Physalis peruviana, mora Rubus spp, guayaba Psidium guajava y heliconias Heliconia spp. En mora se diferenciaron las especies R. glaucus, R. robustus y R. urticifolius, se detectaron duplicados y se encontró alta variabilidad genética en R. glaucus, la especie más importante. En uchuva se encontró alta diversidad y dos accesiones de fruto rojo que se diferenciaron genéticamente de las amarillas y una región geográfica con alta variabilidad. En guayaba los cebadores fueron altamente polimórficos y se encontró alta variabilidad en el Valle del Cauca. En heliconias y especies relacionadas se diferenciaron las familias del orden Zingiberales, algunos subgéneros y variaciones en la especie. La técnica es de bajo costo, utiliza un cebador, no requiere información previa, es altamente polimórfica y diferencia especies en los taxones evaluados.

  14. Análisis comparativo de la aplicación de distintos operadores genéticos al problema de job shop scheduling

    OpenAIRE

    Alfonso, Hugo; Salto, Carolina; Gallard, Raúl Hector

    2000-01-01

    Este trabajo describe un estudio de distintas soluciones al problema del job shop scheduling basado en algoritmos genéticos. La característica fundamental es la utilización de decodificadores incorporada a la representación del cromosuma. El objetivo del trabajo es comparar la performance de los distintos tipos de operadores de crossovers que se pueden usar con esta representación.

  15. La Comunidad Andina frente al reto del acceso a los recursos genéticos y la distribución de beneficios

    Directory of Open Access Journals (Sweden)

    Martha Isabel Gómez Lee

    2012-11-01

    Full Text Available Este artículo estudia las medidas jurídicas y políticas que ha adoptado la Comunidad Andina (CAN sobre el acceso a los recursos genéticos y la distribución de beneficios a la luz de los estudios de equidad en la distribución de beneficios de Bram de Jonge y Niels Louwaar (2009. Según estos autores, el principio de Acceso y Distribución de Beneficios (ADB se justifica por tres asimetrías que están presentes en este asunto. Por un lado, la doble asimetría entre la asignación y la explotación de los recursos y, por otro, la asimetría entre los derechos de propiedad intelectual y los derechos legales de los recursos genéticos y los conocimientos tradicionales. En este contexto, la CAN ratifica los principios de soberanía de los recursos genéticos y de Acceso y Distribución de Beneficios del Convenio sobre Diversidad Biológica. Las principales respuestas que ha dado la CAN al reto del acceso a los recursos genéticos y distribución de beneficios son las Decisiones 391, 486 y 523. En este contexto, el Programa BIOCAN está trabajando en los temas de especial relevancia para la región, dentro de los cuales están los desafíos de asignarle valor a los recursos genéticos y el de la biopiratería.

  16. Plantes, gens, salut i ecologia

    OpenAIRE

    Bueno i Torrens, David, 1965-

    2008-01-01

    Als articles Por als gens i Manipulant els gens vaig parlar de la iniciativa per declarar Catalunya lliure de transgènics i vaig dir que totes les plantes que conreem han estat modificades en el decurs del temps. Els contraris a les plantes transgèniques parlen de possibles efectes negatius sobre la salut i el medi ambient [...].

  17. Microsatélites amplificados al azar (RAM) en estudios de diversidad genética vegetal Random amplified microsatellites (RAM´s) in plant genetic diversity studies

    OpenAIRE

    Jaime Eduardo Muñoz Flórez; Ana Cruz Morillo Coronado; Yacenia Morillo Coronado

    2008-01-01

    Se revisó el uso e importancia, ventajas, desventajas y características de la técnica Microsatélites Amplificados al Azar (RAM) en uchuva Physalis peruviana, mora Rubus spp, guayaba Psidium guajava y heliconias Heliconia spp. En mora se diferenciaron las especies R. glaucus, R. robustus y R. urticifolius, se detectaron duplicados y se encontró alta variabilidad genética en R. glaucus, la especie más importante. En uchuva se encontró alta diversidad y dos accesiones de fruto rojo que se difere...

  18. ENV 322 ASH COURSE Tutorial/UOPHELP

    OpenAIRE

    fgchjklkjhgfd

    2015-01-01

    For more course tutorials visit www.uophelp.com   ENV 322 Complete Class Business - General Business   Gaia Hypothesis. In Chapter 3, the Gaia hypothesis is introduced. It addresses two hypotheses; (1) life, since its inception, has greatly affected the planetary environment and (2) life has altered Earth’s environment in ways that have allowed life to persist. Do you agree with these two hypotheses? Why or why not? Energy and Food Supplies. The und...

  19. Array CGH com a primera opció per al diagnòstic genètic postnatal

    OpenAIRE

    Castells Sarret, Neus

    2015-01-01

    La citogenètica convencional detecta un 3-5% dels pacients amb retard global del desenvolupament / discapacitat intel·lectual (RGD / DI) i / o malformacions congènites (MC). L'amplificació de sondes múltiples dependents de lligació (MLPA) permet incrementar la taxa diagnòstica entre 2,4-5,8%. Actualment els arrays d'hibridació genòmica comparada (aCGH) constitueixen l'eina diagnòstica amb major rendiment en pacients amb RGD / DI, MC i trastorns de l'espectre autista (TEA). L'objectiu del p...

  20. DEL GEN EGOISTA AL GEN ALTRUISTA

    Directory of Open Access Journals (Sweden)

    Oscar José Fernández Galíndez

    2010-06-01

    Full Text Available The Darwinian thought derived from the interpretations and / or rereadings done on Charles Darwin's work, they have generated many controversy. Even the same Darwin demonstrated in many occasions her, doubts on her, before raised ideas, of there that I dress the evolutionism as paradigm, it suggests a serious review in the frame of the emergent thought and in the most igalitarian search of a society and less fragmented.

  1. Detección de Leishmania spp. en base al gen que codifica la proteína HSP20

    Directory of Open Access Journals (Sweden)

    Ana M Montalvo

    2014-10-01

    Full Text Available Objetivos. Explorar una nueva diana para el diagnóstico molecular de Leishmania. Materiales y métodos. Se evaluó la utilidad del gen que codifica la proteína de choque térmico de 20kDa (hsp20 para la detección de Leishmania por medio de la reacción en cadena de la polimerasa (PCR.Se normalizó la PCR y se determinaron los parámetros analíticos, así como la validez y seguridad diagnóstica y la concordancia con la PCR-18S. Se evaluó la PCR-hsp20 con ADN obtenido de un grupo de muestras clínicas de distinta procedencia. Resultados. Los parámetros analíticos resultaron adecuados. La sensibilidad obtenida fue de 86% y la especificidad del 100%, la concordancia con el método de referencia resultó buena (κ = 0,731, lo que apoya su posible uso para el diagnóstico. La posibilidad de identificación posterior de la especie mediante secuenciación del producto amplificado le confiere una ventaja adicional. Conclusiones. Se demuestra la utilidad de este gen como una nueva diana para la detección del género Leishmania. Debido a su potencial, se recomienda mejorar la sensibilidad del procedimiento y realizar su evaluación en diversas regiones endémicas.

  2. Detección de Leishmania spp. en base al gen que codifica la proteína HSP20

    Directory of Open Access Journals (Sweden)

    Ana M. Montalvo

    2014-12-01

    Full Text Available Objetivos. Explorar una nueva diana para el diagnóstico molecular de Leishmania. Materiales y métodos. Se evaluó la utilidad del gen que codifica la proteína de choque térmico de 20kDa (hsp20 para la detección de Leishmania por medio de la reacción en cadena de la polimerasa (PCR.Se normalizó la PCR y se determinaron los parámetros analíticos, así como la validez y seguridad diagnóstica y la concordancia con la PCR-18S. Se evaluó la PCR-hsp20 con ADN obtenido de un grupo de muestras clínicas de distinta procedencia. Resultados. Los parámetros analíticos resultaron adecuados. La sensibilidad obtenida fue de 86% y la especificidad del 100%, la concordancia con el método de referencia resultó buena (κ = 0,731, lo que apoya su posible uso para el diagnóstico. La posibilidad de identificación posterior de la especie mediante secuenciación del producto amplificado le confiere una ventaja adicional. Conclusiones. Se demuestra la utilidad de este gen como una nueva diana para la detección del género Leishmania. Debido a su potencial, se recomienda mejorar la sensibilidad del procedimiento y realizar su evaluación en diversas regiones endémicas.

  3. Structure and Dynamics of the Native HIV-1 Env Trimer

    OpenAIRE

    Munro, James B.; Mothes, Walther

    2015-01-01

    HIV-1/AIDS remains one of the worst pandemics in human history. Despite tremendous efforts, no effective vaccine has been found. Recent reports give new insights into the structure and dynamics of the HIV-1 Env trimer and renew hopes that a better understanding of Env will translate into new vaccine candidates and more-effective antiretroviral therapies.

  4. Parámetros genéticos y fenotípicos para peso al año, circunferencia escrotal y talla en ganado Simmental y Simbrah en México

    OpenAIRE

    José Antonio Torres-Vázquez; Coralia Inés Valentina Manzanilla Pech; Aurelio Borrayo Zepeda; Ángel Ríos-Utrera; Vicente Eliezer Vega-Murillo; Guillermo Martínez-Velázquez; Juan José Baeza Rodríguez; Moisés Montaño-Bermúdez

    2012-01-01

    El objetivo fue estimar los componentes de (co)varianza, heredabilidades y correlaciones genéticas y fenotípicas para peso al año (YW), circunferencia escrotal (SC) y talla (FS) en toros jóvenes Simmental y Simbrah de México. Todas las características se ajustaron a 305 días acorde a los lineamientos descritos por la Federación para el Mejoramiento Genético de los Bovinos de Carne (BIF). El archivo final de datos incluyó 1,949 registros Simmental y 1,259 Simbrah. Para estimar los compones de ...

  5. Métodos y usos agrícolas de la ingeniería genética aplicada al cultivo de arroz

    Directory of Open Access Journals (Sweden)

    Cristina DiazGranados D.

    2012-08-01

    Full Text Available Methods and agricultural uses of genetic engineering applied to rice crop Resumen: En biotecnología de arroz se han logrado avances en transformación genética, con importantes resultados en el mejoramiento genético de variedades elite de las subespecies japónica e índica. Con el propósito de revisar los métodos y los usos agrícolas de la ingeniería genética aplicada al cultivo del arroz, se usaron varias palabras claves en idioma inglés en algunas de las bases de datos de revistas científicas indexadas, disponibles en el Sistema Nacional de Bibliotecas de la Universidad Nacional de Colombia (SINAB, seleccionando documentos publicados entre 2000 y 2011. La base de esta revisión inicial, se complementó con artículos publicados en fechas anteriores, que se consideraron relevantes, debido a que implicaban cambios metodológicos importantes. Desde que se logró producir la primera planta transgénica de arroz a finales de los 80´s, varios protocolos para la transferencia de genes se han empleado con éxito logrando la modificación genética de más de 60 cultivares de arroz. Para ello se han empleado sistemas de transformación tanto directos como indirectos.  Se han realizado modificaciones de rasgos importantes en el cultivo, tales como la resistencia a factores bióticos (insectos, hongos, bacterias, virus, nematodos, tolerancia a factores abióticos (salinidad, sequía, altas y bajas temperaturas, inmersión, y mejoramiento de características agronómicas (calidad nutricional, rendimiento, uso de nutrientes, tolerancia a herbicidas. Palabras claves: Arroz; cultivos transgénicos; factores bióticos; factores abióticos. Abstract: In rice biotechnology advances have been made in genetic transformation, with significant results in breeding elite varieties of japonica and indica subspecies. In order to review the methods and agricultural uses of genetic engineering applied to rice, calves were used several words in English in

  6. Manipular els gens

    OpenAIRE

    Bueno i Torrens, David, 1965-

    2008-01-01

    l'article Por als gens vaig parlar de la iniciativa per declarar Catalunya lliure de transgènics, i vaig dir que els motius que esgrimeixen els promotors no tenen base científica. Aquests motius es poden resumir en tres punts: la negativa a manipular organismes vius, la seguretat alimentària i l'afectació ecològica [...].

  7. Microsatélites amplificados al azar (RAM en estudios de diversidad genética vegetal Random amplified microsatellites (RAM´s in plant genetic diversity studies

    Directory of Open Access Journals (Sweden)

    Jaime Eduardo Muñoz Flórez

    2008-12-01

    Full Text Available Se revisó el uso e importancia, ventajas, desventajas y características de la técnica Microsatélites Amplificados al Azar (RAM en uchuva Physalis peruviana, mora Rubus spp, guayaba Psidium guajava y heliconias Heliconia spp. En mora se diferenciaron las especies R. glaucus, R. robustus y R. urticifolius, se detectaron duplicados y se encontró alta variabilidad genética en R. glaucus, la especie más importante. En uchuva se encontró alta diversidad y dos accesiones de fruto rojo que se diferenciaron genéticamente de las amarillas y una región geográfica con alta variabilidad. En guayaba los cebadores fueron altamente polimórficos y se encontró alta variabilidad en el Valle del Cauca. En heliconias y especies relacionadas se diferenciaron las familias del orden Zingiberales, algunos subgéneros y variaciones en la especie. La técnica es de bajo costo, utiliza un cebador, no requiere información previa, es altamente polimórfica y diferencia especies en los taxones evaluados.The use and importance, advantages, disadvantages and features of the Random Amplified Microsatellites RAMs technique, were reviewed in Cape gooseberry Physalis peruviana, blackberry Rubus spp, guava Psidium guajava and heliconias Heliconia spp. In blackberry, we differentiated the species R. glaucus, R. robustus y R. urticifolius, detected duplicated accessions and found high genetic diversity in R. glaucus, the most important specie. In cape gooseberry we found high diversity and two red fruit accessions genetically differentiated from the yellow fruit ones and a geographical region with high variability. In guava, primers were highly polymorphic and found high variability in Valle del Cauca region. In Heliconia and related species we differentiated families belonging to Zingiberal order, between some sub genera and variation among specie. The technique has low cost of implementation, use a single primer, do not require previous information, is highly

  8. GEN 105 Courses/sanptutorial

    OpenAIRE

    ncbdf

    2015-01-01

    GEN 105 Assignment: Reading and Retention GEN 105 Assignment: Elevator Speech GEN 105 CheckPoint: Technological Tools GEN 105 CheckPoint: Distance Learning I GEN 105 CheckPoint: Distance Learning II GEN 105 CheckPoint: Communicating in Forums GEN 105 Week 2 Discussion Questions GEN 105 Week 4 Discussion Questions GEN 105 Week 6 Discussion Questions GEN 105 Week 8 Discussion Questions GEN 105 Assignment: University Library Article Search GEN 105 Chec...

  9. Caracterización de la diversidad genética en naranja y comparación del polimorfismo de microsatélites amplificados al azar (RAMs usando electroforesis de poliacrilamida y agarosa

    Directory of Open Access Journals (Sweden)

    Muñoz Flores Jaime Eduardo

    2009-12-01

    Full Text Available Se compararon las eficiencias de tres métodos de electroforesis en agarosa y poliacrilamida, usando la cámara pequeña de DNA Sequencing System y cámara grande OWL Sequi-Gen Sequencing Cell, en la detección del polimorfismo en 21 accesiones de naranja (Citrus sinensis con empleo del cebador CGA. El gel de poliacrilamida dio mejor resolución de los productos amplificados vía PCR producidos por RAMs. Este permitió una mejor detección de bandas de ADN polimórficas, lo que facilitó la identificación de la variabilidad genética. La electroforesis en agarosa puede ser más conveniente en otras aplicaciones, debido al bajo costo y fácil aplicación. El estudio de diversidad genética en naranja usando microsatélites RAMs diferenció 51 accesiones en siete grupos con 0.75 de similaridad y 0.25 de heterocigosidad, lo que revela bajo polimorfismo genético. La técnica RAMs permitió agrupar las accesiones en Comunes o Blancas, Navel y Pigmentadas o Sanguinas.

  10. The role of the N-terminal segment of CCR5 in HIV-1 Env-mediated membrane fusion and the mechanism of virus adaptation to CCR5 lacking this segment

    Directory of Open Access Journals (Sweden)

    Kabat David

    2007-08-01

    Full Text Available Abstract Background HIV-1 envelope glycoprotein (Env induces membrane fusion as a result of sequential binding to CD4 and chemokine receptors (CCR5 or CXCR4. The critical determinants of CCR5 coreceptor function are the N-terminal domain (Nt and the second extracellular loop. However, mutations in gp120 adapt HIV-1 to grow on cells expressing the N-terminally truncated CCR5(Δ18 (Platt et al., J. Virol. 2005, 79: 4357–68. Results We have functionally characterized the adapted Env (designated Env(NYP using a quantitative cell-cell fusion assay. The rate of fusion with target cells expressing wild-type CCR5 and the resistance to fusion inhibitors was virtually identical for wild-type Env and Env(NYP, implying that the coreceptor affinity had not increased as a result of adaptation. In contrast, Env(NYP-induced fusion with cells expressing CCR5(Δ18 occurred at a slower rate and was extremely sensitive to the CCR5 binding inhibitor, Sch-C. Resistance to Sch-C drastically increased after pre-incubation of Env(NYP- and CCR5(Δ18-expressing cells at a temperature that was not permissive to fusion. This indicates that ternary Env(NYP-CD4-CCR5(Δ18 complexes accumulate at sub-threshold temperature and that low-affinity interactions with the truncated coreceptor are sufficient for triggering conformational changes in the gp41 of Env(NYP but not in wild-type Env. We also demonstrated that the ability of CCR5(Δ18 to support fusion and infection mediated by wild-type Env can be partially reconstituted in the presence of a synthetic sulfated peptide corresponding to the CCR5 Nt. Pre-incubation of wild-type Env- and CCR5(Δ18-expressing cells with the sulfated peptide at sub-threshold temperature markedly increased the efficiency of fusion. Conclusion We propose that, upon binding the Nt region of CCR5, wild-type Env acquires the ability to productively engage the extracellular loop(s of CCR5 – an event that triggers gp41 refolding and membrane merger

  11. RRE-dependent HIV-1 Env RNA effects on Gag protein expression, assembly and release

    Energy Technology Data Exchange (ETDEWEB)

    López, Claudia S., E-mail: lopezcl@ohsu.edu [Departments of Molecular Microbiology and Immunology, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97239 (United States); Sloan, Rachel; Cylinder, Isabel [Departments of Molecular Microbiology and Immunology, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97239 (United States); Kozak, Susan L.; Kabat, David [Biochemistry and Molecular Biology, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97239 (United States); Barklis, Eric, E-mail: barklis@ohsu.edu [Departments of Molecular Microbiology and Immunology, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97239 (United States)

    2014-08-15

    The HIV-1 Gag proteins are translated from the full-length HIV-1 viral RNA (vRNA), whereas the envelope (Env) protein is translated from incompletely spliced Env mRNAs. Nuclear export of vRNAs and Env mRNAs is mediated by the Rev accessory protein which binds to the rev-responsive element (RRE) present on these RNAs. Evidence has shown there is a direct or indirect interaction between the Gag protein, and the cytoplasmic tail (CT) of the Env protein. Our current work shows that env gene expression impacts HIV-1 Gag expression and function in two ways. At the protein level, full-length Env expression altered Gag protein expression, while Env CT-deletion proteins did not. At the RNA level, RRE-containing Env mRNA expression reduced Gag expression, processing, and virus particle release from cells. Our results support models in which Gag is influenced by the Env CT, and Env mRNAs compete with vRNAs for nuclear export. - Highlights: • At the protein level, full-length HIV-1 Env alters Gag protein expression. • HIV-1 Env RNA expression reduces Gag levels and virus release. • Env RNA effects on Gag are dependent on the RRE. • RRE-containing Env RNAs compete with vRNAs for nuclear export.

  12. RRE-dependent HIV-1 Env RNA effects on Gag protein expression, assembly and release

    International Nuclear Information System (INIS)

    The HIV-1 Gag proteins are translated from the full-length HIV-1 viral RNA (vRNA), whereas the envelope (Env) protein is translated from incompletely spliced Env mRNAs. Nuclear export of vRNAs and Env mRNAs is mediated by the Rev accessory protein which binds to the rev-responsive element (RRE) present on these RNAs. Evidence has shown there is a direct or indirect interaction between the Gag protein, and the cytoplasmic tail (CT) of the Env protein. Our current work shows that env gene expression impacts HIV-1 Gag expression and function in two ways. At the protein level, full-length Env expression altered Gag protein expression, while Env CT-deletion proteins did not. At the RNA level, RRE-containing Env mRNA expression reduced Gag expression, processing, and virus particle release from cells. Our results support models in which Gag is influenced by the Env CT, and Env mRNAs compete with vRNAs for nuclear export. - Highlights: • At the protein level, full-length HIV-1 Env alters Gag protein expression. • HIV-1 Env RNA expression reduces Gag levels and virus release. • Env RNA effects on Gag are dependent on the RRE. • RRE-containing Env RNAs compete with vRNAs for nuclear export

  13. GEN 105 Course tutorial/ indigohelp

    OpenAIRE

    SCD

    2015-01-01

    For more classes visit www.indigohelp.com   GEN 105 Assignment: Reading and Retention GEN 105 Assignment: Elevator Speech GEN 105 CheckPoint: Technological Tools GEN 105 CheckPoint: Distance Learning I GEN 105 CheckPoint: Distance Learning II GEN 105 CheckPoint: Communicating in Forums GEN 105 Week 2 Discussion Questions GEN 105 Week 4 Discussion Questions GEN 105 Week 6 Discussion Questions GEN 105 Week 8 Discussion Questions GEN 105 A...

  14. Variabilidad del gen de la envoltura del VIH-1 en tres grupos humanos con diferentes conductas sexuales de riesgo para adquirir ITS-VIH

    Directory of Open Access Journals (Sweden)

    Carlos Yábar V

    2007-07-01

    Full Text Available Objetivo: Determinar la variabilidad genética del gen de la envoltura porción C2-V3-C3 (env del VIH-1 infectando grupos humanos con diferente conducta sexual de riesgo para adquirir ITS-VIH. Materiales y métodos: Se seleccionaron 50 sujetos infectados con VIH-1 de los cuales 19 fueron hombres trabajadores sexuales (HTS, 8 mujeres trabajadoras sexuales (MTS y 23 sujetos heterosexuales (SH. Se realizó la extracción de ADN genómico y la amplificación del gen env por PCR. Se identificó el subtipo genético por ensayo de movilidad de heterodúplex (HMA y se confirmaron los resultados por análisis filogenético. Asimismo, se realizó el análisis de recombinación intragenética, diversidad y distancia genética en las tres poblaciones. Resultados: Se amplificó el gen env en 49 muestras (98% y se logró secuenciar el fragmento en 40 de ellas. Se observó que el 97,5% de las muestras de VIH fueron subtipo B mientras que una muestra no pudo ser clasificada filogenéticamente. Asimismo, se encontraron pequeños tramos de recombinación en el gen env de VIH en MTS (33%, HTS (43% y SH (45%. El mayor índice de diversidad de nucleótidos (Pi de env se encontró entre las muestras de VIH provenientes de SH y HTS (0,12 y 0,13 respectivamente. Conclusiones: Se encontró una mayor variabilidad genética del gen env de VIH-1 en las poblaciones de HTS y SH, sin embargo, el subtipo genético y la frecuencia de recombinación de este fragmento genético fue similar en los tres grupos estudiados.

  15. Problemática legal generada por la falta de regulación respecto al manejo de bases de datos genéticos de uso forense

    OpenAIRE

    Paz Karaman, Paola Andrea

    2012-01-01

    En Colombia, el INMLCF1administra la base de datos genéticos con fines de investigación criminal, en ella se almacenan perfiles de ADN (datos de carácter personal) de víctimas (de desaparición forzada y familiares de los desaparecidos), de personas relacionadas con la autoría de un delito y de vestigios encontrados en el lugar de los hechos. Sin embargo el marco regulatorio actual no delimita los criterios de uso de la base de datos genéticos con fines de investigación criminal de cara a la p...

  16. Appreciating HIV-1 diversity: subtypic differences in ENV

    Energy Technology Data Exchange (ETDEWEB)

    Gnanakaran, S [Los Alamos National Laboratory; Shen, Tongye [Los Alamos National Laboratory; Lynch, Rebecca M [NON LANL; Derdeyn, Cynthia A [NON LANL

    2008-01-01

    Human immunodeficiency virus type 1 (HIV-1) group M is responsible for the current AIDS pandemic and exhibits exceedingly high levels of viral genetic diversity around the world, necessitating categorization of viruses into distinct lineages, or subtypes. These subtypes can differ by around 35% in the envelope (Env) glycoproteins of the virus, which are displayed on the surface of the virion and are targets for both neutralizing antibody and cell-mediated immune responses. This diversity reflects the remarkable ability of the virus to adapt to selective pressures, the bulk of which is applied by the host immune response, and represents a serious obstacle for developing an effective vaccine with broad coverage. Thus, it is important to understand the underlying biological consequences of inter-subtype diversity. Recent studies have revealed that the HIV-1 subtypes exhibit phenotypic differences that result from subtle differences in Env structure, particularly within the highly immunogenic V3 domain, which participates directly in viral entry. This review will therefore explore current research that describes subtypic differences in Env at the genetic and phenotypic level, focusing in particular on V3, and highlighting recent discoveries about the unique features of subtype C Env, which is the most prevalent subtype globally.

  17. Algoritmos genéticos

    OpenAIRE

    Martínez Páez, José Jesús

    2004-01-01

    Esta técnica se basa en el concepto de evolución a través de selección de los mejores individuos, y de los operadores genéticos de selección, reproducción y mutación. Se trata entonces, de definir un espacio de soluciones para el problema que se quiere solucionar, en una cadena de bits. A esto se le conoce como la codificación del cromosoma, donde cada bit, denominado gen  tiene cierto significado especial. Inicialmente el algoritmo genera al azar muchas de estas cadenas o seres, es decir, un...

  18. Marcadores genéticos dopaminérgicos, serotoninérgicos y de la monoamino-oxidasa (MAO) relacionados con la adicción a los opiáceos, a la cocaína y al alcohol

    OpenAIRE

    Mateu Hernández, César Andrés

    2013-01-01

    Introducción: la genética está experimentando un avance espectacular y se postula como una herramienta importante para aportar nuevos descubrimientos en trastornos complejos como son las adicciones. En el presente trabajo se ha realizado un estudio de asociación genética de polimorfismos VNTR, un estudio familiar y un estudio mixto de asociación genético-familiar en una muestra de policonsumidores con adicción al alcohol, la cocaína y los opiáceos. Material y métodos: en una muestra de 302 po...

  19. Identificació de noves dianes terapèutiques i miRNAs implicats en la resistència al metotrexat mitjançant genòmica funcional

    OpenAIRE

    Mencía Trinchant, Núria

    2013-01-01

    Una de les accepcions de la farmacogenòmica es l'estudi dels efectes d'un tractament farmacològic sobre els nivells d'expressió gènica. Aquesta estratègia inclou la identificació de les variacions de gens individuals, l'avaluació de les interaccions entre els seus productes i la caracterització dels fenotips derivats de la resposta al fàrmac El metotrexat (MTX) és un fàrmac inhibidor de l'enzim dihidrofolat reductasa (DHFR) utilitzat en el tractament del càncer. El treball presentat en...

  20. Insereixen material genètic al nucli cel·lular per a teràpia gènica amb nanodiscs

    OpenAIRE

    Villaverde Corrales, Antonio; Vázquez Gómez, Esther

    2011-01-01

    Investigadors de la UAB han aconseguit encapsular material genètic i alliberar-lo directament dins el nucli de les cèl·lules, per tal de dur a terme teràpia gènica, mitjançant partícules amb forma de disc de la grandària de només un pocs nanòmetres. Els nanodiscs, tal i com els han batejat els investigadors, travessen ràpidament l'interior de la cèl·lula i es concentren en el nucli, de manera que incrementarien l'eficiència del procés de transferència genètica.

  1. Mutationsstudie des Dihydropyrimidin-Dehydrogenase-Gens (DPYD) einer Kontrollpopulation zur Abklärung von DPD-Insuffizienz als genetische Ursache von Fluoropyrimidin-Intoleranz.

    OpenAIRE

    Seck, Katharina

    2006-01-01

    Mithilfe der denaturierenden HPLC wurde eine Mutationsanalyse des gesamten kodierenden Abschnitts des DPYD-Gens durchgeführt. Für jedes Exon und teilweise für einzelne Fragmente eines Exons wurden die optimale DHPLC-Temperatur und der Elutionsgradient anhand von Berechnung sowie experimentell ermittelt, so dass durch das jeweilige Chromatogramm eine definitive Zuordnung zu Wildtyp oder zu spezifischen bekannten Mutanten möglich wurde; stichprobenartige Kontrollen bestätigten in der Sequenzier...

  2. Splicing of Friend Murine Leukemia Virus env-mRNA Enhances Its Ability to Form Polysomes.

    Science.gov (United States)

    Machinaga, Akihito; Ishihara, Syuhei; Shirai, Akiko; Takase-Yoden, Sayaka

    2016-01-01

    Friend murine leukemia virus (MLV) belongs to the gamma retroviruses of the Retroviridae family. The positive-sense RNA of its genome contains a 5' long terminal repeat (LTR), 5' leader sequence, gag, pol, env, and 3' LTR. Transcription from proviral DNA begins from the R region of the 5' LTR and ends at the polyadenylation signal located at the R region of the other end of the 3' LTR. There is a 5' splice site in the 5' leader sequence and a 3' splice site at the 3' end of the pol region. Both full-length unspliced mRNAs and a singly spliced mRNA (env-mRNA) are produced in MLV-infected cells. The MLV Env protein plays important roles both in viral adsorption to host cells and in neuropathogenic disease in MLV-infected mice and rats. Understanding the regulatory mechanisms controlling Env expression is important for determining the functions of the Env protein. We have previously shown that splicing increases env-mRNA stability and translation efficiency. Generally, mRNA polysome formation correlates with translation efficiency. Therefore, here we investigated the effects of env-mRNA splicing on polysome formation to identify mechanisms for Env up-regulation due to splicing. We performed polysome profile analyses using Env-expression plasmids producing spliced or unspliced env-mRNA and showed that the former formed polysomes more efficiently than the latter. Thus, splicing of env-mRNA facilitated polysome formation, suggesting that this contributes to up-regulation of Env expression. We replaced the env region of the expression plasmids with a luciferase (luc) gene, and found that in this case both unspliced and spliced luc-mRNA formed polysomes to a similar extent. Thus, we conclude that whether mRNA polysome formation is affected by splicing depends on the structure of gene in question. PMID:26909075

  3. Identificación genómica de loci y rutas génicas que afectan al crecimiento y deposición grasa en porcino

    OpenAIRE

    Pérez Montarelo, Dafne

    2013-01-01

    El crecimiento y la deposición grasa son algunos de los caracteres económicos más relevantes en la producción porcina, además, el cerdo es utilizado como especie modelo en el estudio de la obesidad y patologías humanas con ella relacionadas, por lo que el estudio de estos caracteres suscita un gran interés. El objetivo general de esta tesis ha sido profundizar en el conocimiento de la base genética de la regulación del crecimiento y la deposición grasa en porcino, identificando genes, rutas g...

  4. GenBank

    Data.gov (United States)

    U.S. Department of Health & Human Services — GenBank is the NIH genetic sequence database, an annotated collection of all publicly available DNA sequences. GenBank is designed to provide and encourage access...

  5. La relación entre los riesgos, la precaución y la responsabilidad en los daños al medio ambiente por la liberación de organismos genéticamente modificados.

    Directory of Open Access Journals (Sweden)

    Sebastián Rebolledo Aguirre

    2012-05-01

    Full Text Available ResumenEl presente trabajo tiene por objeto mostrar la relación teórica y práctica entre la compleja noción de los riesgos; el principio precautorio como norma jurídico-ambiental, y la institución fundamental en todo sistema jurídico de la responsabilidad por daños.de los daños al medio ambiente por la liberación de organismos genéticamente modificados es una utilísima herramienta para determinar el régimen de responsabilidad aplicable, la interpretación de las normas jurídicas involucradas y controlar la discrecionalidad administrativa.Se explica que los riesgos inciertos son el fundamento y la razón que dan operatividad al principio precautorio, y que la funcionalidad de éste en el contexto de los daños al medio ambiente por la liberación de organismos genéticamente modificados es una utilísima herramienta para determinar el régimen de responsabilidad aplicable, la interpretación de las normas jurídicas involucradas y controlar la discrecionalidad administrativa.AbstractThis paper aims to show the theoretical and practical relationship between the complex notion of risk, the precautionary principle as an environmental legal standard, and the legal doctrine of liability for damages.It explains that uncertain risks are the foundation and the reason that makes the precautionary principle operative, and that, in the context of environmental damages caused by the release of GMOs, it is a very useful tool to determine the liability regime and the interpretation of the rules involved, and to control administrative discretion.

  6. Molecular evolution of human immunodeficiency virus env in humans and monkeys: similar patterns occur during natural disease progression or rapid virus passage.

    Science.gov (United States)

    Hofmann-Lehmann, Regina; Vlasak, Josef; Chenine, Agnès-Laurence; Li, Pei-Lin; Baba, Timothy W; Montefiori, David C; McClure, Harold M; Anderson, Daniel C; Ruprecht, Ruth M

    2002-05-01

    Neonatal rhesus macaque 95-3 was inoculated with nonpassaged simian-human immunodeficiency virus strain SHIV-vpu(+), which encodes env of the laboratory-adapted human immunodeficiency virus (HIV) strain IIIB and is considered nonpathogenic. CD4(+) T-cell counts dropped to V. Narayan et al., Virology 256:54-63, 1999; R. Raghavan et al., Brain Pathol. 7:851-861, 1997; E. B. Stephens et al., Virology 231:313-321, 1997). Thus, HIV-IIIB env evolved similarly in three different species; this selection occurred in chronically infected individuals during disease progression as well as after rapid virus passage. We postulate that evolutionary pressure led to the outgrowth of more aggressive viral variants in all three species. PMID:11967343

  7. Identification of the major capsid protein of erythrocytic necrosis virus (ENV) and development of quantitative real-time PCR assays for quantification of ENV DNA.

    Science.gov (United States)

    Purcell, Maureen K; Pearman-Gillman, Schuyler; Thompson, Rachel L; Gregg, Jacob L; Hart, Lucas M; Winton, James R; Emmenegger, Eveline J; Hershberger, Paul K

    2016-07-01

    Viral erythrocytic necrosis (VEN) is a disease of marine and anadromous fish that is caused by the erythrocytic necrosis virus (ENV), which was recently identified as a novel member of family Iridoviridae by next-generation sequencing. Phylogenetic analysis of the ENV DNA polymerase grouped ENV with other erythrocytic iridoviruses from snakes and lizards. In the present study, we identified the gene encoding the ENV major capsid protein (MCP) and developed a quantitative real-time PCR (qPCR) assay targeting this gene. Phylogenetic analysis of the MCP gene sequence supported the conclusion that ENV does not group with any of the currently described iridovirus genera. Because there is no information regarding genetic variation of the MCP gene across the reported host and geographic range for ENV, we also developed a second qPCR assay for a more conserved ATPase-like gene region. The MCP and ATPase qPCR assays demonstrated good analytical and diagnostic sensitivity and specificity based on samples from laboratory challenges of Pacific herring Clupea pallasii The qPCR assays had similar diagnostic sensitivity and specificity as light microscopy of stained blood smears for the presence of intraerythrocytic inclusion bodies. However, the qPCR assays may detect viral DNA early in infection prior to the formation of inclusion bodies. Both qPCR assays appear suitable for viral surveillance or as a confirmatory test for ENV in Pacific herring from the Salish Sea. PMID:27154315

  8. Transcriptional and functional studies of Human Endogenous Retrovirus envelope EnvP(b) and EnvV genes in human trophoblasts

    International Nuclear Information System (INIS)

    HERV (Human Endogenous Retrovirus)-encoded envelope proteins are implicated in the development of the placenta. Indeed, Syncytin-1 and -2 play a crucial role in the fusion of human trophoblasts, a key step in placentation. Other studies have identified two other HERV env proteins, namely EnvP(b) and EnvV, both expressed in the placenta. In this study, we have fully characterized both env transcripts and their expression pattern and have assessed their implication in trophoblast fusion. Through RACE analyses, standard spliced transcripts were detected, while EnvV transcripts demonstrated alternative splicing at its 3′ end. Promoter activity and expression of both genes were induced in forskolin-stimulated BeWo cells and in primary trophoblasts. Although we have confirmed the fusogenic activity of EnvP(b), overexpression or silencing experiments revealed no impact of this protein on trophoblast fusion. Our results demonstrate that both env genes are expressed in human trophoblasts but are not required for syncytialization.

  9. Transcriptional and functional studies of Human Endogenous Retrovirus envelope EnvP(b) and EnvV genes in human trophoblasts

    Energy Technology Data Exchange (ETDEWEB)

    Vargas, Amandine, E-mail: amandine.vargas@voila.fr; Thiery, Maxime, E-mail: thiery.maxime@courrier.uqam.ca; Lafond, Julie, E-mail: lafond.julie@uqam.ca; Barbeau, Benoit, E-mail: barbeau.benoit@uqam.ca

    2012-03-30

    HERV (Human Endogenous Retrovirus)-encoded envelope proteins are implicated in the development of the placenta. Indeed, Syncytin-1 and -2 play a crucial role in the fusion of human trophoblasts, a key step in placentation. Other studies have identified two other HERV env proteins, namely EnvP(b) and EnvV, both expressed in the placenta. In this study, we have fully characterized both env transcripts and their expression pattern and have assessed their implication in trophoblast fusion. Through RACE analyses, standard spliced transcripts were detected, while EnvV transcripts demonstrated alternative splicing at its 3 Prime end. Promoter activity and expression of both genes were induced in forskolin-stimulated BeWo cells and in primary trophoblasts. Although we have confirmed the fusogenic activity of EnvP(b), overexpression or silencing experiments revealed no impact of this protein on trophoblast fusion. Our results demonstrate that both env genes are expressed in human trophoblasts but are not required for syncytialization.

  10. Identification of the major capsid protein of erythrocytic necrosis virus (ENV) and development of quantitative real-time PCR assays for quantification of ENV DNA

    Science.gov (United States)

    Purcell, Maureen K.; Pearman-Gillman, Schuyler; Thompson, Rachel L.; Gregg, Jacob L.; Hart, Lucas M.; Winton, James R.; Emmenegger, Eveline J.; Hershberger, Paul K.

    2016-01-01

    Viral erythrocytic necrosis (VEN) is a disease of marine and anadromous fish that is caused by the erythrocytic necrosis virus (ENV), which was recently identified as a novel member of family Iridoviridae by next-generation sequencing. Phylogenetic analysis of the ENV DNA polymerase grouped ENV with other erythrocytic iridoviruses from snakes and lizards. In the present study, we identified the gene encoding the ENV major capsid protein (MCP) and developed a quantitative real-time PCR (qPCR) assay targeting this gene. Phylogenetic analysis of the MCP gene sequence supported the conclusion that ENV does not group with any of the currently described iridovirus genera. Because there is no information regarding genetic variation of the MCP gene across the reported host and geographic range for ENV, we also developed a second qPCR assay for a more conserved ATPase-like gene region. The MCP and ATPase qPCR assays demonstrated good analytical and diagnostic sensitivity and specificity based on samples from laboratory challenges of Pacific herring Clupea pallasii. The qPCR assays had similar diagnostic sensitivity and specificity as light microscopy of stained blood smears for the presence of intraerythrocytic inclusion bodies. However, the qPCR assays may detect viral DNA early in infection prior to the formation of inclusion bodies. Both qPCR assays appear suitable for viral surveillance or as a confirmatory test for ENV in Pacific herring from the Salish Sea.

  11. Neural Stem Cells as Engraftable Packaging Lines Can Mediate Gene Delivery to Microglia: Evidence from Studying Retroviral env-Related Neurodegeneration

    OpenAIRE

    Lynch, William P.; Sharpe, Arlene H.; Snyder, Evan Y.

    1999-01-01

    The induction of spongiform myeloencephalopathy by murine leukemia viruses is mediated primarily by infection of central nervous system (CNS) microglia. In this regard, we have previously shown that CasBrE-induced disease requires late, rather than early, virus replication events in microglial cells (W. P. Lynch et al., J. Virol. 70:8896–8907, 1996). Furthermore, neurodegeneration requires the presence of unique sequences within the viral env gene. Thus, the neurodegeneration-inducing events ...

  12. Hanstruepera neustonica gen. nov., sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from estuarine water, and emendation of Sediminibacter furfurosus Khan et al. 2007 emend. Kwon et al. 2014, Mangrovimonas yunxiaonensis Li et al. 2013, Antarcticimonas flava Yang et al. 2009 and Hoppeia youngheungensis Kwon et al. 2014.

    Science.gov (United States)

    Hameed, Asif; Shahina, Mariyam; Lai, Wei-An; Lin, Shih-Yao; Liu, You-Cheng; Hsu, Yi-Han; Young, Chiu-Chung

    2015-02-01

    A Gram-staining-negative, strictly aerobic, yellowish-orange, flexirubin-positive, rod-shaped, non-flagellated, non-spore-forming and non-gliding marine bacterium, designated strain CC-PY-50(T), was isolated from estuarine water off Pingtung, Taiwan. The strain produced zeaxanthin as a major carotenoid pigment, and showed highest pairwise 16S rRNA gene sequence similarity to Bizionia hallyeonensis T-y7(T) (93.9 %) followed by Corallibacter vietnamensis KMM 6217(T) (93.8 %), Geojedonia litorea YCS-16(T) (93.7 %) and other members of the family Flavobacteriaceae (5 % of total) fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, C16 : 0 and C16 : 1ω6c and/or C16 : 1ω7c. The DNA G+C content was 37.1 mol% and menaquinone-6 (MK-6) was the sole respiratory quinone. Based on the phylogenetic evidence and several distinguishing phenotypic and chemotaxonomic features, strain CC-PY-50(T) is proposed to represent a novel genus and species of the family Flavobacteriaceae, for which the name Hanstruepera neustonica gen. nov., sp. nov. is proposed. The type strain of the type species Hanstruepera neustonica gen. nov., sp. nov. is CC-PY-50(T) ( = JCM 19743(T) = BCRC 80747(T)). Emended descriptions of the species Sediminibacter furfurosus, Mangrovimonas yunxiaonensis, Antarcticimonas flava and Hoppeia youngheungensis are also proposed. PMID:25351879

  13. Algoritmo Genético aplicado al problema de programación en procesos tecnológicos de maquinado con ambiente Flow Shop

    Directory of Open Access Journals (Sweden)

    José Eduardo Márquez Delgado

    2012-01-01

    Full Text Available Debido a las limitaciones de las técnicas de optimización convencionales, en el siguiente trabajo se presenta una metaheurística basada en un algoritmo genético (AG, para resolver problemas de programación de tipo flow shop, con el objetivo de minimizar el tiempo de finalización de todos los trabajos, más conocido como makespan. Este problema, considerado de difícil solución, es típico de la optimización combinatoria y se presenta en talleres con tecnología de maquinado, donde existen máquinas herramientas convencionales y se fabrican diferentes tipos de piezas que tienen en común una misma ruta tecnológica (orden del proceso. La solución propuesta se probó con problemas clásicos publicados por otros autores, obteniéndose resultados satisfactorios en cuanto a la calidad de las soluciones encontradas y el tiempo de cómputo empleado.

  14. NMobTec-EnvEdu: M-Learning System for Environmental Education

    Science.gov (United States)

    Cavus, Nadire

    2008-01-01

    This paper introduced the implementation of a New Mobile Technologies and Environmental Education System (NMobTec-EnvEdu) designed for m-learning environments. The NMobTec-EnvEdu system has been developed to provide environmental education in a collaborative framework to undergraduate students through the Internet using mobile phones. The study…

  15. GEN 105 UOP course tutorial/tutorialoutlet

    OpenAIRE

    NARESH 1

    2015-01-01

    For more course tutorials visit www.tutorialoutlet.com     GEN 105 Assignment: Reading and Retention GEN 105 Assignment: Elevator Speech GEN 105 CheckPoint: Technological Tools GEN 105 CheckPoint: Distance Learning I GEN 105 CheckPoint: Distance Learning II GEN 105 CheckPoint: Communicating in Forums GEN 105 Week 2 Discussion Questions GEN 105 Week 4 Discussion Questions GEN 105 Week 6 Discussion Questions GEN 105 Week 8 Discussion...

  16. Gen quimérico que utiliza el gen o cDNA de la insulina, en especial para terapia génica de la diabetes

    OpenAIRE

    Bosch i Tubert, Fàtima; Valera Abril, Alfons

    2001-01-01

    Gen quimérico que utiliza el gen o CDNA de la insulina, en especial para terapia génica de la diabetes. El gen quimérico está dirigido por un promotor o fusión de promotores, los cuales preferentemente son regulables y están activados por el proceso diabético. Preferentemente, se obtiene por fusión del gen de la insulina humana al promotor de la PEPCK (p-enolpiruvato carboxiquinasa). Dicho promotor (fragmento - 460 pb a + 73 pb) está fusionado a la zona 5'' franqueante del gen de la insulina ...

  17. Parsers funcionales genéricos

    OpenAIRE

    Martínez López, Pablo E.; Nestares, Gustavo A.

    1996-01-01

    En este trabajo se propone el uso de técnicas avanzadas de programación funcional para ofrecer una solución genérica al problema de parsing. Este problema consiste en que, dada una lista de tokens que representan una estructura, debe obtenerse una representación elaborada de la misma. La solución propuesta es genérica en dos sentidos. Primero, la técnica de diseño utilizada consiste en dar una biblioteca de combinadores, los cuales pueden combinarse para escribir soluciones a problemas com...

  18. Low Genetic Diversity Among Garlic (Allium sativum L. Accessions Detected Using Random Amplified Polymorphic DNA (RAPD Escasa Diversidad Genética entre Accesiones de Ajo (Allium sativum L. Detectada Mediante ADN Polimórfico Amplificado al Azar (RAPD

    Directory of Open Access Journals (Sweden)

    Mario Paredes C

    2008-03-01

    Full Text Available Garlic (Allium sativum L. is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. The objective of this study was to determine the genetic diversity of 65 garlic clones collected in Chile and introduced from different countries, by using RAPD (Random Amplified Polymorphic DNA. Fourty random primers of 10 mers generated a total of 398 bands with an 87% of polymorphism. Each primer amplified between two and 20 bands. The size of the fragments obtained fluctuated between 3200 and 369 bp. The results showed that the clones analyzed had a genetic similarity rate of 94%. In addition, 70% of them were clustered in one major group. However, in spite of that situation several clones have different agronomic characteristicsEl ajo (Allium sativum L. es una especie de propagación vegetativa, que presenta una amplia variabilidad morfológica. Los clones de esta especie tienen una adaptación específica a diferentes regiones agroclimáticas. El objetivo de este estudio fue determinar la diversidad genética existente en 65 clones de ajos colectados en Chile e introducidos desde diferentes países, utilizando RAPD (ADN Polimórfico Amplificado al Azar. Para esta evaluación se utilizaron 40 partidores de 10-mers. Los partidores generaron entre dos y 20 bandas, observándose un alto número de patrones con bandas múltiples. Los fragmentos generados difieren en su tamaño entre 3.200 y 369 pb. Los partidores generaron 398 bandas, de las cuales un 87% fueron polimórficas. El análisis estadístico realizado detectó una similitud genética alta, de un 94% entre las accesiones evaluadas, donde aproximadamente un 70% de los clones formaron un grupo homogéneo. Sin embargo, este grupo incluye clones que presentan diferentes características agronómicas

  19. Estado emocional y creencias de salud en personas con agregación familiar al cáncer de mama que requieren consejo genético

    OpenAIRE

    Cabrera Torres, Esther

    2008-01-01

    Durante el año 2006, se produjeron 1.703.000 defunciones a causa del cáncer en Europa. El cáncer más frecuente fue el de mama, siendo el 13,5% de todos los casos de neoplasias. Sin embargo en España, los pacientes diagnosticados con cáncer de mama presentan mayor supervivencia que otros tipos tumorales; 94% al año, 84% a los 3 años y 78% a los 5 años. La mejora de la supervivencia es uno de los indicadores más importantes de la eficacia del sistema asistencial en la lucha contra el cáncer y ...

  20. Comparison of the Immunogenicities of HIV-1 Mutants Based on Structural Modification of env

    Institute of Scientific and Technical Information of China (English)

    Jian-hui NIE; Chun-tao ZHANG; Hui-hui CHONG; Xue-ling WU; Chu-yu LIU; Yu WU; Chen-yan ZHAO; Lin-qi ZHANG; You-Chun WANG

    2008-01-01

    Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around Nab epitopes and deletions of variable regions in env.The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γELISPOT.Overall,five mutants(dWt,M2,M5-2,M5-1 and dM7)induced highed neutralization activities for both pseudoviruses than plasmid Wt,while only two of the mutants(dWt and M5-2)showed significant differences(P<0.05).Two mutants(M2 and dM2)induced more Env-specific T cells than plasmid Wt.Statistically however,significance was only reached for mutant M2.Thus,properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.

  1. Inhibition of Human Immunodeficiency Virus Type 1 Env-Mediated Fusion by DC-SIGN

    OpenAIRE

    Nobile, Cinzia; Moris, Arnaud; Porrot, Françoise; Sol-Foulon, Nathalie; Schwartz, Olivier

    2003-01-01

    DC-SIGN, a lectin expressed on dendritic cell and macrophage subsets, binds to human immunodeficiency virus Env glycoproteins, allowing capture of viral particles. Captured virions either infect target cells or are efficiently transmitted to lymphocytes. Cellular mechanisms underlying the effects of DC-SIGN remain poorly understood. Here we have analyzed the effects of DC-SIGN on viral entry and on syncytium formation induced by Env glycoproteins. The lectin enhanced susceptibility to viral i...

  2. PANACEA English automatically acquired lexicon for ENV domain: Subcategorization Frames and Lexical Semantic classes for nouns

    OpenAIRE

    University of Cambridge. Department of Theoretical and Applied Linguistics; Universitat Pompeu Fabra. Institut Universitari de Ling????stica Aplicada (IULA)

    2013-01-01

    This is a domain-specific lexicon for English for environment (ENV) domain. This lexicon contain both, subcategorization frames for verbs and lexical semantic classes for nouns. This lexicon has been automatically created using PANACEA webservices using crawled data. The crawled data was obtained crawling web pages that were automatically detected to be in the English language and were automatically classified as relevant to the ENV domain. Data collection took place in the summer of 2011.

  3. Evidence for a new form of retroviral env transcript in leukemic and normal mouse lymphoid cells

    International Nuclear Information System (INIS)

    Murine leukemia virus-related RNA species were examined in a set of radiation-induced T-cell leukemias from BALB/c mice. No evidence was found for linkage of viral long terminal repeat-derived (U5) sequences to information of host origin. A novel class of 2-kilobase (kb) env-related transcripts, about 1kb shorter than normal viral env messenger, was found in all the leukemias. All of the 2-kb transcripts contained sequences homologous to the xenotropic virus-related env sequences in the Friend spleen focus-forming virus, representing the N-terminal portion of gp70. In two of the leukemias, these transcripts were found to contain both ecotropic p15E and U3 sequences in addition to the xenotropic gp70-related sequence. These two leukemias, but not others in which ecotropic sequences were absent from the 2-kb RNA, harbored several copies of a specific class of env recombinant proviruses. These proviruses possessed full-size env genes and were submethylated, as shown by SmaI and XmaI digests of proviral DNA. Low levels of 2-kb RNA were found in normal thymocytes from strains BALB/c, AKR, and 129 but not from congenic 129 GIX- mice. It is possible that the 2-kb RNA may originate by a novel splicing step that removes portions of the gp70 and p15E sequences from full-length env transcripts

  4. Proposal of Sphaerimonospora cavernae gen. nov., sp. nov. and transfer of Microbispora mesophila (Zhang et al., 1998) to Sphaerimonospora mesophila comb. nov. and Microbispora thailandensis (Duangmal et al., 2012) to Sphaerimonospora thailandensis comb. nov.

    Science.gov (United States)

    Mingma, Ratchanee; Duangmal, Kannika; Také, Akira; Inahashi, Yuki; O Mura, Satoshi; Takahashi, Yo Ko; Matsumoto, Atsuko

    2016-04-01

    The actinomycete strain N74T, isolated from cave soil, was studied using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain N74T formed a stable, distinct lineage cluster together with Microbispora thailandensis NN276T (99.3 % similarity) and Microbispora mesophila JCM 3151T (97.5 %). Strain N74T was observed to produce single spherical spores on aerial mycelium as reported for M. mesophila JCM 3151T and M. thailandensis NN276T but different from other known species of the genus Microbispora, which are characterized by pairs of spores on aerial hyphae. Multilocus sequence analyses based on concatenated partial gyrB, rpoB, atpD, recA and 16S rRNA gene sequences showed a clear distinction of strain N74T, M. mesophila JCM 3151T and M. thailandensis NN276T from other members of the genus Microbispora, although the chemotaxonomic characteristics of strain N74T were similar to the genus Microbispora; the cell wall contained meso-diaminopimelic acid and the whole-cell hydrolysate contained madurose as the diagnostic sugar. The major menaquinone was MK-9(H4). The fatty acid profile contained iso-C16 : 0. On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain N74T is assigned to a novel species of a new genus, for which the name Sphaerimonospora cavernae gen. nov., sp. nov. is proposed. The type strain of Sphaerimonospora cavernae is N74T ( = BCC 77604T = NBRC 111481T). It is also proposed that M. mesophila and M. thailandensis be transferred to this genus as Sphaerimonospora mesophila comb. nov. (type strain JCM 3151T = NBRC 14179T = DSM 43048T) and Sphaerimonospora thailandensis comb. nov. (type strain NN276T = BCC 41490T = NBRC 107569T), respectively. PMID:26822211

  5. Adaptación al español del cuestionario Vecú et Sante Perçue de l'Adolescent (VSP-A: una medida genérica de calidad de vida para adolescentes

    Directory of Open Access Journals (Sweden)

    Serra-Sutton Vicky

    2002-01-01

    Full Text Available Fundamentos: En la última década se han desarrollado diversas medidas de calidad de vida relacionada con la salud para uso exclusivo en niños/as y adolescentes. No obstante, existen pocos instrumentos de estas características adaptados en España. El Vecú et Sante Perçue de l'adolescent (VSP-A es un instrumento genérico de calidad de vida relacionada con la salud para adolescentes de 11 a 17 años desarrollado en Francia. El objetivo de este estudio fue adaptar al español el VSP-A, como primera fase para la obtención del cuestionario. Métodos: Se adaptó la versión del VSP-A de 39 preguntas siguiendo la metodología de traducción directa e inversa incluyendo: 2 traducciones al español, puntuación del grado de dificultad (0 min-10 máx y clasificación de equivalencia semántica y cultural, 2 reuniones y discusión en paneles de adolescentes, así como reuniones de consenso del equipo de investigación. Finalmente se realizó una traducción inversa (retro-traducción al francés y se administró la versión final pre-test en la prueba piloto. Resultados: La mayoría de las preguntas se clasificaron como equivalentes (24 sobre 39. Tras las reuniones con adolescentes se modificaron algunas preguntas. Tras la retro-traducción, 3 preguntas necesitaron cambios menores. Conclusiones: La versión española del VSP-A parece semántica y culturalmente equivalente a la versión original en francés y adecuada para adolescentes en España. La sencillez de las preguntas, los comentarios de los adolescentes y la participación de los autores originales en el proceso de adaptación ha permitido obtener una versión pre-test adecuada. La siguiente fase del estudio es la comprobación de la fiabilidad y validez. Se espera que el VSP-A sea de utilidad para medir la calidad de vida relacionada con la salud en encuestas de salud o como instrumento de cribado en colegios o centros de atención primaria en nuestro medio.

  6. Linfohistiocitosis hemofagocítica, el espectro desde la enfermedad genética al síndrome de activación macrofágica

    Directory of Open Access Journals (Sweden)

    Oscar Porras

    2011-06-01

    Full Text Available El compromiso de la regulación de la inflamación produce activación excesiva y expansión de macrófagos y linfocitos T que desencadenan una reacción inflamatoria severa, sin vías naturales de control. Los trastornos hemofagocíticos son la traducción clínica de este proceso inflamatorio. La linfohistiocitosis hemofagocítica se refiere a todas las variantes de esta patología, y el síndrome de activación macrofágica, a la variante asociada con enfermedad autoinmune. Los casos primarios se asocian con la forma familiar autosómica recesiva y los secundarios con inmunodeficiencias primarias, infección, malignidad y enfermedades autoinmunes. El principal distintivo de este grupo de patologías es la proliferación agresiva de macrófagos e histiocitos que fagocitan otras células sanguíneas. La reducción en la actividad de las células NK produce un aumento en la activación y expansión de linfocitos T, los cuales producen grandes cantidades de citoquinas. Las citoquinas inducen activación de macrófagos y células dendríticas, infiltración tisular y producción de interleuquinas, lo que genera una reacción inflamatoria severa, responsable del daño tisular y de las manifestaciones clínicas. El curso clínico se caracteriza principalmente por fiebre prolongada, hepatoesplenomegalia y citopenias. Los estudios de laboratorio muestran aumento de ferritina, triglicéridos e hipofibrinogenemia. La hemofagocitosis en médula ósea está presente en más del 80% de los casos al diagnóstico. El tratamiento está dirigido contra el linfocito T y los histiocitos hiperactivados, combinando quimioterapia con inmunosupresores y, en algunos casos, trasplante de células madre hematopoyéticas. Este tratamiento ha producido un cambio en la sobrevida de los pacientes. El protocolo de tratamiento HLH-2004 es una guía que estandariza el tratamiento, combinando etopósido, dexametazona y ciclosporina A. En Costa Rica se han reportado 60 casos en

  7. Antibody to gp41 MPER alters functional properties of HIV-1 Env without complete neutralization.

    Directory of Open Access Journals (Sweden)

    Arthur S Kim

    2014-07-01

    Full Text Available Human antibody 10E8 targets the conserved membrane proximal external region (MPER of envelope glycoprotein (Env subunit gp41 and neutralizes HIV-1 with exceptional potency. Remarkably, HIV-1 containing mutations that reportedly knockout 10E8 binding to linear MPER peptides are partially neutralized by 10E8, producing a local plateau in the dose response curve. Here, we found that virus partially neutralized by 10E8 becomes significantly less neutralization sensitive to various MPER antibodies and to soluble CD4 while becoming significantly more sensitive to antibodies and fusion inhibitors against the heptad repeats of gp41. Thus, 10E8 modulates sensitivity of Env to ligands both pre- and post-receptor engagement without complete neutralization. Partial neutralization by 10E8 was influenced at least in part by perturbing Env glycosylation. With unliganded Env, 10E8 bound with lower apparent affinity and lower subunit occupancy to MPER mutant compared to wild type trimers. However, 10E8 decreased functional stability of wild type Env while it had an opposite, stabilizing effect on MPER mutant Envs. Clade C isolates with natural MPER polymorphisms also showed partial neutralization by 10E8 with altered sensitivity to various gp41-targeted ligands. Our findings suggest a novel mechanism of virus neutralization by demonstrating how antibody binding to the base of a trimeric spike cross talks with adjacent subunits to modulate Env structure and function. The ability of an antibody to stabilize, destabilize, partially neutralize as well as alter neutralization sensitivity of a virion spike pre- and post-receptor engagement may have implications for immunotherapy and vaccine design.

  8. Role of the Cytoplasmic Tail of Ecotropic Moloney Murine Leukemia Virus Env Protein in Fusion Pore Formation

    OpenAIRE

    Melikyan, Grigory B.; Markosyan, Ruben M.; Brener, Sofya A.; Rozenberg, Yanina; Cohen, Fredric S.

    2000-01-01

    Fusion between cells expressing envelope protein (Env) of Moloney murine leukemia virus and target cells were studied by use of video fluorescence microscopy and electrical capacitance measurements. When the full-length 632-amino-acid residue Env was expressed, fusion did not occur at all for 3T3 cells as target and only somewhat for XC6 cells. Expression of Env 616*—a construct of Env with the last 16 amino acid residues (617 to 632; the R peptide) deleted from its C terminus to match the pr...

  9. Cleavage-Independent HIV-1 Env Trimers Engineered as Soluble Native Spike Mimetics for Vaccine Design

    Directory of Open Access Journals (Sweden)

    Shailendra Kumar Sharma

    2015-04-01

    Full Text Available Viral glycoproteins mediate entry by pH-activated or receptor-engaged activation and exist in metastable pre-fusogenic states that may be stabilized by directed rational design. As recently reported, the conformationally fixed HIV-1 envelope glycoprotein (Env trimers in the pre-fusion state (SOSIP display molecular homogeneity and structural integrity at relatively high levels of resolution. However, the SOSIPs necessitate full Env precursor cleavage, which requires endogenous furin overexpression. Here, we developed an alternative strategy using flexible peptide covalent linkage of Env subdomains to produce soluble, homogeneous, and cleavage-independent Env mimics, called native flexibly linked (NFL trimers, as vaccine candidates. This simplified design avoids the need for furin co-expression and, in one case, antibody affinity purification to accelerate trimer scale-up for preclinical and clinical applications. We have successfully translated the NFL design to multiple HIV-1 subtypes, establishing the potential to become a general method of producing native-like, well-ordered Env trimers for HIV-1 or other viruses.

  10. Parenteral Administration of Capsule Depolymerase EnvD Prevents Lethal Inhalation Anthrax Infection.

    Science.gov (United States)

    Negus, David; Vipond, Julia; Hatch, Graham J; Rayner, Emma L; Taylor, Peter W

    2015-12-01

    Left untreated, inhalation anthrax is usually fatal. Vegetative forms of Bacillus anthracis survive in blood and tissues during infection due to elaboration of a protective poly-γ-D-glutamic acid (PDGA) capsule that permits uncontrolled bacterial growth in vivo, eventually leading to overwhelming bacillosis and death. As a measure to counter threats from multidrug-resistant strains, we are evaluating the prophylactic and therapeutic potential of the PDGA depolymerase EnvD, a stable and potent enzyme which rapidly and selectively removes the capsule from the surface of vegetative cells. Repeated intravenous administration of 10 mg/kg recombinant EnvD (rEnvD) to mice infected with lethal doses of B. anthracis Ames spores by inhalation prevented the emergence of symptoms of anthrax and death; all animals survived the 5-day treatment period, and 70% survived to the end of the 14-day observation period. In contrast to results in sham-treated animals, the lungs and spleen of rEnvD-dosed animals were free of gross pathological changes. We conclude that rEnvD has potential as an agent to prevent the emergence of inhalation anthrax in infected animals and is likely to be effective against drug-resistant forms of the pathogen. PMID:26438506

  11. Capacidad predictiva del modelo BCRAPro frente al profesional de enfermería en la selección de candidatos a estudio genético de cáncer de mama u ovario hereditario

    OpenAIRE

    Benito-Aracil, Llúcia; Yagüe Muñoz, Carmen; Iglesias Casals, Sílvia; Salinas Masdeu, Mònica; Teulé Vega, Àlex; Lázaro García, Conxi; Blanco Guillermo, Ignacio

    2010-01-01

    Objetivo: Comparar la capacidad predictiva del modelo predictivo BRCAPro y de los profesionales de enfermería con distintos niveles de formación/experiencia en la identificación de familias susceptibles de ser estudiadas genéticamente dada su historia personal y familiar de cáncer de mama. Método: Estudio descriptivo en el que 2 enfermeras con diferente grado de formación en consejo genético han estimado la probabilidad de ser portador de mutación en los genes BRCA1/BRCA2 de 157 familias. Se ...

  12. SHIV-1157i and passaged progeny viruses encoding R5 HIV-1 clade C env cause AIDS in rhesus monkeys

    Directory of Open Access Journals (Sweden)

    Siddappa Nagadenahalli B

    2008-10-01

    Full Text Available Abstract Background Infection of nonhuman primates with simian immunodeficiency virus (SIV or chimeric simian-human immunodeficiency virus (SHIV strains is widely used to study lentiviral pathogenesis, antiviral immunity and the efficacy of AIDS vaccine candidates. SHIV challenges allow assessment of anti-HIV-1 envelope responses in primates. As such, SHIVs should mimic natural HIV-1 infection in humans and, to address the pandemic, encode HIV-1 Env components representing major viral subtypes worldwide. Results We have developed a panel of clade C R5-tropic SHIVs based upon env of a Zambian pediatric isolate of HIV-1 clade C, the world's most prevalent HIV-1 subtype. The parental infectious proviral clone, SHIV-1157i, was rapidly passaged through five rhesus monkeys. After AIDS developed in the first animal at week 123 post-inoculation, infected blood was infused into a sixth monkey. Virus reisolated at this late stage was still exclusively R5 tropic and mucosally transmissible. Here we describe the long-term follow-up of this initial cohort of six monkeys. Two have remained non-progressors, whereas the other four gradually progressed to AIDS within 123–270 weeks post-exposure. Two progressors succumbed to opportunistic infections, including a case of SV40 encephalitis. Conclusion These data document the disease progression induced by the first mucosally transmissible, pathogenic R5 non-clade B SHIV and suggest that SHIV-1157i-derived viruses, including the late-stage, highly replication-competent SHIV-1157ipd3N4 previously described (Song et al., 2006, display biological characteristics that mirror those of HIV-1 clade C and support their expanded use for AIDS vaccine studies in nonhuman primates.

  13. Human Antibodies that Recognize Novel Immunodominant Quaternary Epitopes on the HIV-1 Env Protein.

    Directory of Open Access Journals (Sweden)

    Mark D Hicar

    Full Text Available Numerous broadly neutralizing antibodies (Abs target epitopes that are formed or enhanced during mature HIV envelope formation (i.e. quaternary epitopes. Generally, it is thought that Env epitopes that induce broadly neutralizing Abs are difficult to access and poorly immunogenic because of the characteristic oligomerization, conformational flexibility, sequence diversity and extensive glycosylation of Env protein. To enhance for isolation of quaternary epitope-targeting Abs (QtAbs, we previously used HIV virus-like particles (VLPs to bind B cells from long-term non-progressor subjects to identify a panel of monoclonal Abs. When expressed as recombinant full-length Abs, a subset of these novel Abs exhibited the binding profiles of QtAbs, as they either failed to bind to monomeric Env protein or showed much higher affinity for Env trimers and VLPs. These QtAbs represented a significant proportion of the B-cell response identified with VLPs. The Ab genes of these clones were highly mutated, but they did not neutralize common HIV strains. We sought to further define the epitopes targeted by these QtAbs. Competition-binding and mapping studies revealed these Abs targeted four separate epitopes; they also failed to compete for binding by Abs to known major neutralizing epitopes. Detailed epitope mapping studies revealed that two of the four epitopes were located in the gp41 subunit of Env. These QtAbs bound pre-fusion forms of antigen and showed differential binding kinetics depending on whether oligomers were produced as recombinant gp140 trimers or as full-length Env incorporated into VLPs. Antigenic regions within gp41 present unexpectedly diverse structural epitopes, including these QtAb epitopes, which may be targeted by the naturally occurring Ab response to HIV infection.

  14. Human Antibodies that Recognize Novel Immunodominant Quaternary Epitopes on the HIV-1 Env Protein.

    Science.gov (United States)

    Hicar, Mark D; Chen, Xuemin; Sulli, Chidananda; Barnes, Trevor; Goodman, Jason; Sojar, Hakimuddin; Briney, Bryan; Willis, Jordan; Chukwuma, Valentine U; Kalams, Spyros A; Doranz, Benjamin J; Spearman, Paul; Crowe, James E

    2016-01-01

    Numerous broadly neutralizing antibodies (Abs) target epitopes that are formed or enhanced during mature HIV envelope formation (i.e. quaternary epitopes). Generally, it is thought that Env epitopes that induce broadly neutralizing Abs are difficult to access and poorly immunogenic because of the characteristic oligomerization, conformational flexibility, sequence diversity and extensive glycosylation of Env protein. To enhance for isolation of quaternary epitope-targeting Abs (QtAbs), we previously used HIV virus-like particles (VLPs) to bind B cells from long-term non-progressor subjects to identify a panel of monoclonal Abs. When expressed as recombinant full-length Abs, a subset of these novel Abs exhibited the binding profiles of QtAbs, as they either failed to bind to monomeric Env protein or showed much higher affinity for Env trimers and VLPs. These QtAbs represented a significant proportion of the B-cell response identified with VLPs. The Ab genes of these clones were highly mutated, but they did not neutralize common HIV strains. We sought to further define the epitopes targeted by these QtAbs. Competition-binding and mapping studies revealed these Abs targeted four separate epitopes; they also failed to compete for binding by Abs to known major neutralizing epitopes. Detailed epitope mapping studies revealed that two of the four epitopes were located in the gp41 subunit of Env. These QtAbs bound pre-fusion forms of antigen and showed differential binding kinetics depending on whether oligomers were produced as recombinant gp140 trimers or as full-length Env incorporated into VLPs. Antigenic regions within gp41 present unexpectedly diverse structural epitopes, including these QtAb epitopes, which may be targeted by the naturally occurring Ab response to HIV infection. PMID:27411063

  15. Kommentarer til arbejdsdokument ENV/04/27. Dokument fra "Antibiotic resistance marker genes working group"

    DEFF Research Database (Denmark)

    Kjellsson, Gøsta

    2012-01-01

    "DMU har modtaget og vurderet det fremsendte dokument ENV/04/27 og bilag (mail fra Skov- og Naturstyrelsen d. 14-10-2004) om anvendelsen af antibiotikaresistensmarkører (ARM) hos GMO. Vi har tidligere flere gange kommenteret anvendelsen af antibiotikaresistens (se f.eks. brev til Skov- og Naturst...

  16. Targeted Isolation of Antibodies Directed against Major Sites of SIV Env Vulnerability.

    Science.gov (United States)

    Mason, Rosemarie D; Welles, Hugh C; Adams, Cameron; Chakrabarti, Bimal K; Gorman, Jason; Zhou, Tongqing; Nguyen, Richard; O'Dell, Sijy; Lusvarghi, Sabrina; Bewley, Carole A; Li, Hui; Shaw, George M; Sheng, Zizhang; Shapiro, Lawrence; Wyatt, Richard; Kwong, Peter D; Mascola, John R; Roederer, Mario

    2016-04-01

    The simian immunodeficiency virus (SIV) challenge model of lentiviral infection is often used as a model to human immunodeficiency virus type 1 (HIV-1) for studying vaccine mediated and immune correlates of protection. However, knowledge of the structure of the SIV envelope (Env) glycoprotein is limited, as is knowledge of binding specificity, function and potential efficacy of SIV antibody responses. In this study we describe the use of a competitive probe binding sort strategy as well as scaffolded probes for targeted isolation of SIV Env-specific monoclonal antibodies (mAbs). We isolated nearly 70 SIV-specific mAbs directed against major sites of SIV Env vulnerability analogous to broadly neutralizing antibody (bnAb) targets of HIV-1, namely, the CD4 binding site (CD4bs), CD4-induced (CD4i)-site, peptide epitopes in variable loops 1, 2 and 3 (V1, V2, V3) and potentially glycan targets of SIV Env. The range of SIV mAbs isolated includes those exhibiting varying degrees of neutralization breadth and potency as well as others that demonstrated binding but not neutralization. Several SIV mAbs displayed broad and potent neutralization of a diverse panel of 20 SIV viral isolates with some also neutralizing HIV-2(7312A). This extensive panel of SIV mAbs will facilitate more effective use of the SIV non-human primate (NHP) model for understanding the variables in development of a HIV vaccine or immunotherapy. PMID:27064278

  17. Selected HIV-1 Env trimeric formulations act as potent immunogens in a rabbit vaccination model

    DEFF Research Database (Denmark)

    Heyndrickx, Leo; Stewart-Jones, Guillaume; Jansson, Marianne Bendixen; Schuitemaker, Hanneke; Bowles, Emma; Buonaguro, Luigi; Grevstad, Berit; Vinner, Lasse; Vereecken, Katleen; Parker, Joe; Ramaswamy, Meghna; Biswas, Priscilla; Vanham, Guido; Scarlatti, Gabriella; Fomsgaard, Anders

    2013-01-01

    Ten to 30% of HIV-1 infected subjects develop broadly neutralizing antibodies (bNAbs) during chronic infection. We hypothesized that immunizing rabbits with viral envelope glycoproteins (Envs) from these patients may induce bNAbs, when formulated as a trimeric protein and in the presence of an...

  18. Diversidad genética en pitahaya (Hylocereus undatus Haworth. Britton y Rose)

    OpenAIRE

    Juan Porfirio Legaria Solano; María Elisa Alvarado Cano; Ricardo Gaspar Hernández

    2005-01-01

    Se evaluó la variabilidad genética con marcadores RAPD (Polimorfismos en el ADN Amplificados al Azar) en 50 colectas de pitahaya (Hylocereus undatus Haworth, Britton and Rose) provenientes de nueve estados de México y una colecta de Colombia que se incluyó como testigo. Se detectó alta variabilidad genética (polimorfismo entre colectas de 92.5 %) en las poblaciones de pitahaya. Las huellas genéticas obtenidas permitieron la identificación individual de cada material genét...

  19. Antigenic properties of a transport-competent influenza HA/HIV Env chimeric protein

    International Nuclear Information System (INIS)

    The transmembrane subunit (gp41) of the HIV Env glycoprotein contains conserved neutralizing epitopes which are not well-exposed in wild-type HIV Env proteins. To enhance the exposure of these epitopes, a chimeric protein, HA/gp41, in which the gp41 of HIV-1 89.6 envelope protein was fused to the C-terminus of the HA1 subunit of the influenza HA protein, was constructed. Characterization of protein expression showed that the HA/gp41 chimeric proteins were expressed on cell surfaces and formed trimeric oligomers, as found in the HIV Env as well as influenza HA proteins. In addition, the HA/gp41 chimeric protein expressed on the cell surface can also be cleaved into 2 subunits by trypsin treatment, similar to the influenza HA. Moreover, the HA/gp41 chimeric protein was found to maintain a pre-fusion conformation. Interestingly, the HA/gp41 chimeric proteins on cell surfaces exhibited increased reactivity to monoclonal antibodies against the HIV Env gp41 subunit compared with the HIV-1 envelope protein, including the two broadly neutralizing monoclonal antibodies 2F5 and 4E10. Immunization of mice with a DNA vaccine expressing the HA/gp41 chimeric protein induced antibodies against the HIV gp41 protein and these antibodies exhibit neutralizing activity against infection by an HIV SF162 pseudovirus. These results demonstrate that the construction of such chimeric proteins can provide enhanced exposure of conserved epitopes in the HIV Env gp41 and may represent a novel vaccine design strategy for inducing broadly neutralizing antibodies against HIV

  20. Caracterización de la diversidad genética en naranja y comparación del polimorfismo de microsatélites amplificados al azar (RAMs usando electroforesis de poliacrilamida y azarosa Characterization of the genetic diversity in orange, and comparison of polymorphism in randomly-amplifed microsatellites (RAMs, using polyacrylamide and agarose electrophoresis

    Directory of Open Access Journals (Sweden)

    Ana Cruz Morillo Coronado

    2009-10-01

    Full Text Available Se compararon las eficiencias de tres métodos de electroforesis en agarosa y poliacrilamida, usando la cámara pequeña de DNA Sequencing System y cámara grande OWL Sequi-Gen Sequencing Cell, en la detección del polimorfismo en 21 accesiones de naranja (Citrus sinensis con empleo del cebador CGA. El gel de poliacrilamida dio mejor resolución de los productos amplificados vía PCR producidos por RAMs. Este permitió una mejor detección de bandas de ADN polimórficas, lo que facilitó la identificación de la variabilidad genética. La electroforesis en agarosa puede ser más conveniente en otras aplicaciones, debido al bajo costo y fácil aplicación. El estudio de diversidad genética en naranja usando microsatélites RAMs diferenció 51 accesiones en siete grupos con 0.75 de similaridad y 0.25 de heterocigosidad, lo que revela bajo polimorfismo genético. La técnica RAMs permitió agrupar las accesiones en Comunes o Blancas, Navel y Pigmentadas o Sanguinas.We compared the efficiency of three methods of agarose and polyacrylamide electrophoresis (using the small tank of the DNA Sequencing System and the large OWL Sequi-Gen Sequencing Cell, for the detection of polymorphism in 21 accessions of orange (Citrus sinensis, using the primer CGA. The polyacrylamide gel gave better resolution of the PCR-amplified RAM products. This method allowed better detection of polymorphic DNA bands, facilitating the identification of genetic variability. The agarose electrophoresis may be more convenient in other applications, due to its low cost and easy implementation. The study of genetic diversity in orange using RAMs separated 51 accessions into seven groups with 0.75 similarity, and 0.25 heterozygosity, revealing low genetic polymorphism. The RAMs technique grouped the accessions into “Common or White”, “Navel” and “Pigmented or “Sanguine”.

  1. Gen-Gen- und Gen-Umwelt-Interaktionsanalysen bei Kindern der multrizentrischen Allergie Studie

    OpenAIRE

    Deindl, Philipp

    2005-01-01

    Atopische Erkrankungen wie Asthma, atopische Dermatitis und allergische Rhinitis sind komplexe, multifaktorielle Erkrankungen. Diese Arbeit untersuchte Gen-Gen- und Gen-Umweltinteraktionen von insgesamt acht Polymorphismen in fünf Kandidatengenen. In einer großen deutschen Geburtskohorte (Multizentrische Allergie Studie, MAS 90) mit longitudinalen klinischen und serologischen Daten wurde der Effekt von Polymorphismen in Genen, die für Interleukin-13 (IL-13), Interleukin-4 (IL-4) und dessen...

  2. Conservation of a proteinase cleavage site between an insect retrovirus (gypsy) Env protein and a baculovirus envelope fusion protein

    International Nuclear Information System (INIS)

    The predicted Env protein of insect retroviruses (errantiviruses) is related to the envelope fusion protein of a major division of the Baculoviridae. The highest degree of homology is found in a region that contains a furin cleavage site in the baculovirus proteins and an adjacent sequence that has the properties of a fusion peptide. In this investigation, the homologous region in the Env protein of the gypsy retrovirus of Drosophila melanogaster (DmegypV) was investigated. Alteration of the predicted DmegypV Env proteinase cleavage site from RIAR to AIAR significantly reduced cleavage of Env in both Spodoptera frugiperda (Sf-9) and D. melanogaster (S2) cell lines. When the predicted DmegypV Env cleavage site RIAR was substituted for the cleavage sequence RRKR in the Lymantria dispar nucleopolyhedrovirus fusion protein (LD130) sequence, cleavage of the hybrid LD130 molecules still occurred, although at a reduced level. The conserved 21-amino acid sequence just downstream of the cleavage site, which is thought to be the fusion peptide in LD130, was also characterized. When this sequence from DmegypV Env was substituted for the homologous sequence in LD130, cleavage still occurred, but no fusion was observed in either cell type. In addition, although a DmegypV-Env-green fluorescent protein construct localized to cell membranes, no cell fusion was observed

  3. XIAO Pei-gen

    Institute of Scientific and Technical Information of China (English)

    2013-01-01

    <正>Academician of Chinese Academy of Engineering Editor-in-chief of Chinese Herbal Medicines(CHM)Honorary director of Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing100193,China Tel/Fax:+86-10-62894462 E-mail:xiaopg@public.bta.net.cn Professor XIAO Pei-gen is the founder of the Institute of Medicinal Plant Development(IMPLAD),Chinese Academy of Medical Sciences(CAMS).He is also one of the founders and leading

  4. Cálcul estructural d'un Observatori Astronómic situat a La Palma (Canàries), segons Normativa ENV 1993-1-1

    OpenAIRE

    Lacroizette i Mañas, Xavier

    2004-01-01

    Premi al millor Projecte de Fi de Carrera presentat durant el curs 2004-2005 en l'àmbit de Construcció que atorga el GREMI DE CONSTRUCTORS D'OBRES El present Projecte de Fi de Carrera està dedicat al disseny i càlcul estructural d’un dels complexes que composen l’Observatori Astronòmic del "Roque de los Muchachos", situat a la illa de la Palma (Canàries). Els diferents càlculs de la estructura metàl·lica s’han realitzat segons la normativa experimental europea ENV 1993-1-1, més coneg...

  5. GEN 499 ASH course tutorial/tutorialoutlet

    OpenAIRE

    NARESH 34

    2015-01-01

    For more course tutorials visit www.tutorialoutlet.com       GEN 499 Week 1 DQ 1 Final Research Paper Topic and Plan (Ash) GEN 499 Week 1 DQ 2 Social Media (Ash) GEN 499 Week 2 DQ 1 Professional Resume and Cover Letter (Ash) GEN 499 Week 2 Assignment Critiquing Internet Sources (Ash) GEN 499 Week 3 DQ 1 Social Capital (Ash) GEN 499 Week 3 DQ 2 Federal Policy (Ash) GEN 499 Week 3 Assignment Annotated Bibliography (Ash) GEN 499 Week 4 DQ 1...

  6. Gen quimérico que comprende el gen o ADNc de la fructoquinasa para el desarrollo de aproximaciones terapéuticas a la intolerancia hereditaria a la fructosa

    OpenAIRE

    Bosch i Tubert, Fàtima; Feliu Albiñana, Juan Emilio; Pujol i Altarriba, Anna; Sánchez Gutiérrez, Julio César

    2003-01-01

    Gen quimérico para el desarrollo de aproximaciones terapéuticas a la intolerancia hereditaria a la fructosa. Comprende un promotor unido al gen o al ADNc de la fructoquinasa. Dicho gen quimérico se inserta en una célula hepática, permitiendo la expresión de fructoquinasa. También se refiere a un animal transgénico cuyo genoma comprende dicho gen quimérico y desarrollando, dicho animal transgénico, alteraciones asociadas a la intolerancia hereditaria a la fructosa tras la administración de fru...

  7. La genómica de las plantas

    OpenAIRE

    Cánovas, Francisco M.

    2009-01-01

    La mejora vegetal es una actividad tan antigua como la propia agricultura. El estudio de las variedades genéticas de la vegetación permite conocer en profundidad sus funciones y mecanismos para localizar los beneficios alimenticios, textiles, cosméticos o combustibles que la naturaleza ha puesto al servicio del ser humano.

  8. Genética molecular del alcoholismo

    OpenAIRE

    Mauricio Rey-Buitrago

    2015-01-01

    El alcoholismo es una patología psiquiátrica compleja y de origen multifactorial en la que el factor genético explica alrededor del 50 % del fenómeno. Son numerosos los genes que se han asociado a esta enfermedad, pero su aporte individual es mínimo y contradictorio. Estos genes operan a través de características intermedias como la impulsividad y la sensibilidad al alcohol, lo que hace compleja la definición del fenotipo del alcoholismo. Los estudios de asociación de SNPs, de asociación a to...

  9. La gens Licinia y el Nordeste peninsular. Una aproximación al estudio de las formas de propiedad y de gestión de un rico patrimonio familiar

    Directory of Open Access Journals (Sweden)

    Berni, Piero

    2005-12-01

    Full Text Available In the last years, either progress in landscape archaeology as well as studies on the epigraphy of production areas, have contribute to increase our volume of information. However, such headway not always has come along with an improvement in the data interpretation, in other words, in the study of the complex forms of landownership and management of resources in Roman Spain. We believed it is necessary to integrate diverse types of existent documentation sources, and we propose in the present case to study a working model focus on the gens Licinia. The importance of this gens in the Northeast of the Peninsula and the outstanding volume of information preserved allow us to put forward a series of methodological and historical reflections we judge significant.En los últimos años, tanto el progreso de la arqueología del paisaje, como especialmente el de los estudios sobre la epigrafía de la producción, han contribuido a incrementar nuestro volumen de información, aunque ello no siempre ha ido acompañado de un progreso en la interpretación de los datos, es decir, en el estudio de las complejas formas de propiedad de la tierra y de la gestión de sus recursos en la Hispania romana. Creemos necesario, por tanto, integrar en este estudio los diversos tipos de fuentes documentales existentes y proponer un modelo de trabajo centrado en el caso de la gens Licinia, cuya importancia en el Nordeste peninsular por el notable volumen de información conservada nos permite plantear algunas reflexiones metodológicas e históricas que creemos significativas.

  10. EnvMine: A text-mining system for the automatic extraction of contextual information

    Directory of Open Access Journals (Sweden)

    de Lorenzo Victor

    2010-06-01

    Full Text Available Abstract Background For ecological studies, it is crucial to count on adequate descriptions of the environments and samples being studied. Such a description must be done in terms of their physicochemical characteristics, allowing a direct comparison between different environments that would be difficult to do otherwise. Also the characterization must include the precise geographical location, to make possible the study of geographical distributions and biogeographical patterns. Currently, there is no schema for annotating these environmental features, and these data have to be extracted from textual sources (published articles. So far, this had to be performed by manual inspection of the corresponding documents. To facilitate this task, we have developed EnvMine, a set of text-mining tools devoted to retrieve contextual information (physicochemical variables and geographical locations from textual sources of any kind. Results EnvMine is capable of retrieving the physicochemical variables cited in the text, by means of the accurate identification of their associated units of measurement. In this task, the system achieves a recall (percentage of items retrieved of 92% with less than 1% error. Also a Bayesian classifier was tested for distinguishing parts of the text describing environmental characteristics from others dealing with, for instance, experimental settings. Regarding the identification of geographical locations, the system takes advantage of existing databases such as GeoNames to achieve 86% recall with 92% precision. The identification of a location includes also the determination of its exact coordinates (latitude and longitude, thus allowing the calculation of distance between the individual locations. Conclusion EnvMine is a very efficient method for extracting contextual information from different text sources, like published articles or web pages. This tool can help in determining the precise location and physicochemical

  11. PANACEA English automatically acquired lexicon for ENV domain: Lexical Semantic classes for nouns

    OpenAIRE

    Universitat Pompeu Fabra. Institut Universitari de Ling????stica Aplicada (IULA)

    2012-01-01

    This is a domain-specific lexicon of for English for environtment (ENV) domain. This lexicon contains a set of nouns classified into seven different semantic classes. It has been automatically created using the PANACEA web services for noun classification and the crawled data for this domain and language, previously annotated with FreeLing tagger. The crawled data was obtained crawling web pages that were automatically detected to be in the English language and were automatically classified a...

  12. Gen-Umwelt-Interaktionen und Gen-Umwelt-Korrelationen bei psychiatrischen Erkrankungen

    Directory of Open Access Journals (Sweden)

    Winkler D

    2010-01-01

    Full Text Available Die seit Langem bestehende Frage, in welchem Ausmaß Anlage und Umwelt zu psychologischen Merkmalen und psychiatrischen Erkrankungen beitragen, wird durch Ergebnisse von Zwillingsstudien und in letzter Zeit durch Untersuchungen des Zusammenhangs zwischen molekulargenetischen Merkmalen und Umwelteinflüssen bereichert. Eine Gen-Umwelt-Interaktion liegt dann vor, wenn genetische Faktoren die Auswirkungen von Umweltbedingungen modulieren. Die Genetik kann weiters die Wahrscheinlichkeit der Exposition gegenüber bestimmten Umwelteinflüssen verändern, was als Gen- Umwelt-Korrelation bezeichnet wird. Beide Phänomene liegen aber häufig gleichzeitig vor, was eine besondere Herausforderung für die Konzeption von wissenschaftlichen Studien darstellt.

  13. Receptor-Triggered but Alkylation-Arrested Env of Murine Leukemia Virus Reveals the Transmembrane Subunit in a Prehairpin Conformation

    OpenAIRE

    Wallin, Michael; Ekström, Maria; Garoff, Henrik

    2006-01-01

    A central feature of the prevailing model for retrovirus fusion is conversion of the transmembrane (TM) subunit from a prehairpin to a hairpin-like structure. The fusion inhibition of many retroviruses, except murine leukemia virus (MLV), with peptides corresponding to interacting regions in the hairpin supports the model. MLV fusion is controlled by isomerization of the intersubunit disulfide in Env. We show here that TM peptides bind to MLV Env that has been arrested at an intermediate stag...

  14. Antibodies to a conformational epitope on gp41 neutralize HIV-1 by destabilizing the Env spike

    Science.gov (United States)

    Lee, Jeong Hyun; Leaman, Daniel P.; Kim, Arthur S.; Torrents de La Peña, Alba; Sliepen, Kwinten; Yasmeen, Anila; Derking, Ronald; Ramos, Alejandra; de Taeye, Steven W.; Ozorowski, Gabriel; Klein, Florian; Burton, Dennis R.; Nussenzweig, Michel C.; Poignard, Pascal; Moore, John P.; Klasse, Per Johan; Sanders, Rogier W.; Zwick, Michael B.; Wilson, Ian A.; Ward, Andrew B.

    2015-09-01

    The recent identification of three broadly neutralizing antibodies (bnAbs) against gp120-gp41 interface epitopes has expanded the targetable surface on the HIV-1 envelope glycoprotein (Env) trimer. By using biochemical, biophysical and computational methods, we map the previously unknown trimer epitopes of two related antibodies, 3BC315 and 3BC176. A cryo-EM reconstruction of a soluble Env trimer bound to 3BC315 Fab at 9.3 Å resolution reveals that the antibody binds between two gp41 protomers, and neutralizes the virus by accelerating trimer decay. In contrast, bnAb 35O22 binding to a partially overlapping quaternary epitope at the gp120-gp41 interface does not induce decay. A conserved gp41-proximal glycan at N88 was also shown to play a role in the binding kinetics of 3BC176 and 3BC315. Finally, our data suggest that the dynamic structure of the Env trimer influences exposure of bnAb epitopes.

  15. Characterization of BIV Env core: Implication for mechanism of BIV-mediated cell fusion

    International Nuclear Information System (INIS)

    Entry of lentiviruses, such as human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV), requires folding of two heptad repeat regions (HR1 and HR2) of gp41 into a trimer-of-hairpins, which subsequently brings virus and cell membrane into fusion. This motif is a generalized feature of viral fusion proteins and has been exploited in generating antiviral fusion agents. In the present paper, we report structural characters of Env protein from another lentivirus, bovine immunodeficiency virus (BIV), which contributes to a good animal model of HIV. BIV HR1 and HR2 regions are predicted by two different programs and expressed separately or conjointly in Escherichia coli. Biochemical and biophysical analyses show that the predicted HRs of BIV Env can form a stable trimer-of-hairpins or six-helix bundle just like that formed by feline immunodeficiency virus Env. Cell fusion assay demonstrates that the HR2 peptide of BIV can efficiently inhibit the virus-mediated cell fusion

  16. Expression of the env gene from the avian endogenous retrovirus ALVE and regulation by miR-155.

    Science.gov (United States)

    Hu, Xuming; Zhu, Wenqi; Chen, Shihao; Liu, Yangyang; Sun, Zhen; Geng, Tuoyu; Wang, Xiaoyan; Gao, Bo; Song, Chengyi; Qin, Aijian; Cui, Hengmi

    2016-06-01

    Endogenous retroviruses (ERVs) are important retroelements that reside in host genomes. However, ERV expression patterns and regulatory mechanisms are poorly understood. In this study, chicken embryo fibroblasts (CEFs) and MSB1 cells infected with Marek's disease virus (MDV) exhibited significantly increased expression of env from the endogenous retrovirus ALVE. In contrast, env expression was significantly lower in CEF and MSB1 cells infected with exogenous avian leukosis virus J (ALVJ) at the early infection stage. Furthermore, env was found to be ubiquitously expressed in various chicken tissues, with high expression in certain tissues at 2 days of age and low levels in most tissues, including immune organs (thymus, spleen and bursa) as well as the brain and heart, at 35 days of age. Sequence analysis revealed miR-155 target sites in env transcripts, which was verified using a firefly luciferase reporter assay, and treatment with miR-155 agomir significantly decreased levels of env transcripts in MSB1 and CEF cells. Together, these findings suggest that the env gene from the endogenous retrovirus ALVE is regulated by miR-155. PMID:27016933

  17. El psicoanálisis como envés de la ley // Psychoanalysis as the underside of the law

    Directory of Open Access Journals (Sweden)

    Paula Winkler

    2011-06-01

    Full Text Available El psicoanálisis se ocupa del sujeto. El restituir al sujeto en su decir verdad a partir del inconsciente (y de la palabra aparece, desde el inicio, epistemológicamente, como el envés de la ley. La ley se debe a lo público y aquél a lo privado. La distinción entre "público" y "privado" deviene, reglada, desde el Derecho Romano —primer Digesto jurídico—. Pero la praxis analítica, como política institucional, al recomponer el lazo social y presentificar la tensión entre el sujeto y el derecho, permite que el sujeto aborde lo real "haciendo uso" de la norma y que se ubique, además así, en la dimensión simbólica social de la ley. // Psychoanalysis deals with the subject. The return to the subject in saying truth as from the unconscious (and the word appears, from the beginning, as the epistemological reverse of the law. This latter is due to the public whereas psychoanalysis is due to the private. The distinction between "public" and "private" becomes, regulated, from the Roman law - first-Legal Digest. Yet, the analytical practice as an institutional policy, while rebuilding the social ties and materializing tension between the subject and the rule of law, allows the subject to dwell into the the real thing "making use" of the rule of law and placing it also in the social/ symbolic dimension of the law

  18. Funciones del gen relacionado a la latencia (gen LR) del herpes virus bovino tipo 1 (BoHV-1) y otras proteínas expresadas durante la latencia

    OpenAIRE

    S.E. Pérez

    2009-01-01

    El herpesvirus bovino tipo 1 (BoHV-1) causa diversos síndromes clínicos en el ganado. Al igual que otros alfa-herpesvirus, el ciclo de vida del BoHV-1 puede dividirse en tres etapas: infección aguda, latencia y reactivación. Durante la infección aguda, BoHV-1 expresa una cascada de genes altamente regulados. Sin embargo, durante la latencia, la expresión de genes virales se ve restringida al gen relacionado a la latencia (gen LR) y al ORF-E. El gen LR es poliadenilado y sufre empalmes alterna...

  19. Low pH Is Required for Avian Sarcoma and Leukosis Virus Env-Induced Hemifusion and Fusion Pore Formation but Not for Pore Growth

    OpenAIRE

    Melikyan, G. B.; Barnard, R. J. O.; Markosyan, R M; Young, J. A. T.; Cohen, F S

    2004-01-01

    Binding of avian sarcoma and leukosis virus (ASLV) to its cognate receptor on the cell surface causes conformational changes in its envelope protein (Env). It is currently debated whether low pH is required for ASLV infection. To elucidate the role of low pH, we studied the association between ASLV subgroup B (ASLV-B) and liposomes and fusion between effector cells expressing Env from ASLV-A and ASLV-B and target cells expressing cognate receptors. Neither EnvA nor EnvB promoted cell-cell fus...

  20. Complementation of diverse HIV-1 Env defects through cooperative subunit interactions: a general property of the functional trimer

    Directory of Open Access Journals (Sweden)

    Salzwedel Karl

    2009-08-01

    Full Text Available Abstract Background The HIV-1 Env glycoprotein mediates virus entry by catalyzing direct fusion between the virion membrane and the target cell plasma membrane. Env is composed of two subunits: gp120, which binds to CD4 and the coreceptor, and gp41, which is triggered upon coreceptor binding to promote the membrane fusion reaction. Env on the surface of infected cells is a trimer consisting of three gp120/gp41 homo-dimeric protomers. An emerging question concerns cooperative interactions between the protomers in the trimer, and possible implications for Env function. Results We extended studies on cooperative subunit interactions within the HIV-1 Env trimer, using analysis of functional complementation between coexpressed inactive variants harboring different functional deficiencies. In assays of Env-mediated cell fusion, complementation was observed between variants with a wide range of defects in both the gp120 and gp41 subunits. The former included gp120 subunits mutated in the CD4 binding site or incapable of coreceptor interaction due either to mismatched specificity or V3 loop mutation. Defective gp41 variants included point mutations at different residues within the fusion peptide or heptad repeat regions, as well as constructs with modifications or deletions of the membrane proximal tryptophan-rich region or the transmembrane domain. Complementation required the defective variants to be coexpressed in the same cell. The observed complementation activities were highly dependent on the assay system. The most robust activities were obtained with a vaccinia virus-based expression and reporter gene activation assay for cell fusion. In an alternative system involving Env expression from integrated provirus, complementation was detected in cell fusion assays, but not in virus particle entry assays. Conclusion Our results indicate that Env function does not require every subunit in the trimer to be competent for all essential activities. Through

  1. Drosophila germline invasion by the endogenous retrovirus gypsy: involvement of the viral env gene.

    Science.gov (United States)

    Pelisson, A; Mejlumian, L; Robert, V; Terzian, C; Bucheton, A

    2002-10-01

    The endogenous retrovirus gypsy is expressed at high levels in mutant flamenco female flies. Gypsy viral particles extracted from such flies can infect naive flamenco individuals raised in the presence of these extracts mixed into their food. This results in the integration of new proviruses into the germline genome. These proviruses can then increase their copy number by (1) expression in the flamenco female somatic cells, (2) transfer into the oocyte and (3) integration into the genome of the progeny. Surprisingly, unlike the infection observed in the feeding experiments, this strategy of endogenous proviral multiplication does not seem to involve the expression of the viral env gene. PMID:12225916

  2. Genética molecular del alcoholismo

    Directory of Open Access Journals (Sweden)

    Mauricio Rey-Buitrago

    2015-07-01

    Full Text Available El alcoholismo es una patología psiquiátrica compleja y de origen multifactorial en la que el factor genético explica alrededor del 50 % del fenómeno. Son numerosos los genes que se han asociado a esta enfermedad, pero su aporte individual es mínimo y contradictorio. Estos genes operan a través de características intermedias como la impulsividad y la sensibilidad al alcohol, lo que hace compleja la definición del fenotipo del alcoholismo. Los estudios de asociación de SNPs, de asociación a todo el genoma, de expresión y epigenéticos han identificado una amplia gama de variantes genéticas y epigenéticas, blancos para los estudios de susceptibilidad, diagnóstico y tratamiento farmacológico. Actualmente se comprenden mucho más estas relaciones y el desarrollo rápido de nuevas metodologías de estudio promete continuar este proceso, así como la generación de algoritmos de diagnóstico, prevención y tratamientos más acertados y confiables.

  3. Organismos modificados genéticamente en la alimentación humana

    OpenAIRE

    Barros Fernández, Paula

    2014-01-01

    El presente trabajo trata la controversia del tema de los organismos modificados genéticamente (OMG). Se mencionan los beneficios que aporta la ingeniería genética y también los principales riesgos y preocupaciones existentes en torno al consumo de los alimentos modificados genéticamente, reportando casos de estudios que así lo constatan.Se tratan temas como seguridad alimentaria, legislación y normativas de etiquetado de estos nuevos alimentos, señalando su relación con la salud. Además, se ...

  4. El papel del gen del transportador de serotonina en los trastornos de la conducta alimentaria

    OpenAIRE

    Sandra Hernández-Muñoz; Beatriz Camarena-Medellín

    2014-01-01

    Introducción: Al sistema serotoninérgico se lo ha implicado en la regulación del estado de ánimo y en la conducta alimentaria, por lo que el gen del transportador de serotonina (SLC6A4) es un buen candidato para el desarrollo de los trastornos de la conducta alimentaria (TCA). La mayoría de los estudios genéticos en los TCA se han centrado principalmente en un polimorfismo, el denominado 5-HTTLPR del gen SLC6A4. Objetivo: Realizar una revisión de los estudios de asociación entre el 5-HTTLPR y...

  5. Estudio preliminar de la expresión del mensaje genético del transportador de serotonina en células mononucleares de sangre periférica en pacientes con dependencia al alcohol con y sin depresión mayor comórbida

    Directory of Open Access Journals (Sweden)

    Enrique Becerril-Villanueva

    2011-01-01

    Full Text Available La Encuesta Nacional de Adicciones 2008 reportó que en México existen 39 millones de personas que consumen alcohol y 4.8 millones presentan dependencia. A nivel mundial varios estudios indican que los pacientes con dependencia al alcohol (40 a 50% presentan comorbilidad con algún tipo de padecimiento psiquiátrico. La función serotoninérgica es un mediador clave en los estados de ánimo, la impulsividad y las conductas adictivas, entre ellas elconsumo de alcohol. Se ha reportado que el consumo excesivo de alcohol etílico disminuye los niveles de serotonina, aumenta la frecuencia de disparo de las neuronas serotoninérgicas en el núcleo del rafé y aumenta los niveles de serotonina en el núcleo accumbens. Las técnicas de biología molecular han permitido identificar factores de riesgo genético y se han seleccionado genes candidatos del sistema serotoninérgico, siendo uno de ellos el gen para el transportador de serotonina (5-HTT, el cual se ha demostrado que se encuentra asociado tanto a una mayor susceptibilidad para el establecimiento de la dependencia al alcohol como a la depresión mayor. Los receptores del sistema serotoninérgico como el 5-HTT, el 5-HT1 y el 5-HT2 se expresan tanto en las células del Sistema Nervioso como en las células del sistema inmunológico, lo que sugiere una similitud funcional de ambos sistemas. Es por ello que las células mononucleares de sangre periférica (PBMC han sido utilizadas como un modelo de estudio en los trastornos de dependencia al alcohol y en los psiquiátricos. El objetivo de este estudio fue evaluar los niveles de expresión del gen 5-HTT en células mononucleares de sangre periférica de pacientes con dependencia al alcohol con y sin depresión mayor comórbida. En el Servicio de Consulta Externa del Centro de Ayuda a Alcohólicos y Familiares (CAAF y en el Centro de Alcohólicos y Drogadictos «Carrasco» se evaluaron 70 pacientes durante el periodo comprendido entre febrero y

  6. A Molecular Epidemiology Analysis of HIV in Shenzhen and HIV Env Gene Variation Replication Analysis

    Institute of Scientific and Technical Information of China (English)

    CHEN Lin(陈琳); FENG Tiejian(冯铁建); LI Liangcheng(李良成); HE Jianfan(何建凡)

    2002-01-01

    Objective: To analyze molecular trends of the HIV epidemic in Shenzhen.Methods: Serum collected from Shenzhen AIDS patientsbetween 1992-1999 was analyzed using molecular techniques.DNA fragments of the HIV-1 Env gene were amplified bynested PCR from uncultured peripheral blood mononuclearcells (PBMCs) from these serum samples. The C2-C3 region ofthe Env gene was sequenced and analyzed. Specific high-riskbehaviors were also analyzed.Results: We found that the transmission of HIV in the citywas mainly through sexual behaviors (46.0%). There werefour HIV-1 subtypes: B', B, C and E with 6.31%, 7.95%,3.09% and 8.92% gene divergence inside each subtype inShenzhen. These results suggested that epidemic times were 6,8, 3 and 9 respectively. The main epidemic subtypes were Eand B strains. AIDS patient's antigenic variation was slightlyhigher than that of HIV infected individuals.Conclusion: Surveillance data reflect trends and theepidemic time of HIV, which will be useful for policy makersto formulate effective strategies of HIV/AIDS prevention andcontrol in Shenzhen.

  7. Equivalencia clínica entre el rociador nasal de propionato de fluticasona genérico y comercial en pacientes con rinitis alérgica: Clinical equival ence between generic and branded fluticasone propionate nasal spray in patients with alergic rhinitis

    Directory of Open Access Journals (Sweden)

    Vikki Brandi

    2009-10-01

    Full Text Available Objetivo. Establecer la equivalencia clínica de un rociador nasal de propionato de fluticasona (RNF genérico comparado con dos formulas farmacéuticas comerciales del mismo producto (Flonase® y Flixonase® durante la estación de polinización del cedro de montaña (Juniperus ashei en Texas, EEUU. Materiales y métodos. Estudio multicéntrico, aleatorizado, doble ciego, controlado con placebo, en grupos paralelos diseñado para investigar la seguridad y eficacia de RNF (200 mcg una vez al día, Flonase® (200 mcg una vez al día y Flixonase® (200 mcg una vez al día, comparados con placebo, administrados por 13 a 15 días. Los pacientes registraron diariamente, en la mañana y en la tarde, sus síntomas nasales totales (SNT. La variable de desenlace primaria fue la suma de SNT en la mañana y tarde + 1. Las variables de desenlace secundarias fueron los SNT AM + 1 y SNT PM + 1, y la evaluación de seguridad. Resultados. No se observó diferencia estadísticamente significativa en ningún día de estudio, ni en todo el periodo de tratamiento, ni al punto de final entre SNT promedio tanto de Flonase® como Flixonase® y RNF. La equivalencia clínica entre RNF y Flonase® (cociente= 0,98; intervalo de confianza [IC] al 90%, 0,91 a 1,06, y entre RNF y Flixonase® (cociente= 1,02; IC 90%, 0,94 a 1,10 fue demostrada tanto para la variable de desenlace primaria como para las otras variables de eficacia. Conclusiones. Estos resultados respaldan la equivalencia clínica entre RNF 200 mcg una vez al día tanto con Flonase® como Flixonase® en el tratamiento de rinitis alérgica estacional.

  8. Effective writing tasks and feedback for the iGen

    OpenAIRE

    Buyse, Kris

    2010-01-01

    Teaching foreign language writing often lacks adjustments to the requirements of today’s students of the “Internet Generation" (iGen): usually teachers set a —not extremely inspiring— theme, a deadline and then return a discouraging, manually underlined and/or annotated text without systematic labeling; at the end the annotated document is stored both by the teacher and the student in a bottomless box. Whereas earlier student generations were educated and trained to process conscientiously al...

  9. Function of conserved histidine-243 in phosphatase activity of EnvZ, the sensor for porin osmoregulation in Escherichia coli.

    OpenAIRE

    Hsing, W; Silhavy, T J

    1997-01-01

    EnvZ and OmpR are the sensor and response regulator proteins of a two-component system that controls the porin regulon of Escherichia coli in response to osmolarity. Three enzymatic activities are associated with EnvZ: autokinase, OmpR kinase, and OmpR-phosphate (OmpR-P) phosphatase. Conserved histidine-243 is critical for both autokinase and OmpR kinase activities. To investigate its involvement in OmpR-P phosphatase activity, histidine-243 was mutated to several other amino acids and the ph...

  10. Structures of HIV-1-Env V1V2 with broadly neutralizing antibodies reveal commonalities that enable vaccine design

    OpenAIRE

    Gorman, Jason; Soto, Cinque; Yang, Max M.; Davenport, Thaddeus M.; Guttman, Miklos; Robert T Bailer; Chambers, Michael; Chuang, Gwo-Yu; DeKosky, Brandon J.; Doria-Rose, Nicole A.; Druz, Aliaksandr; Ernandes, Michael J.; Georgiev, Ivelin S.; Jarosinski, Marissa C.; Joyce, M. Gordon

    2015-01-01

    Broadly neutralizing antibodies (bNAbs) against HIV-1-Env V1V2 arise in multiple donors. However, atomic-level interactions had only been determined with antibodies from a single donor, making commonalities in recognition uncertain. Here we report the co-crystal structure of V1V2 with antibody CH03 from a second donor and model Env interactions of antibody CAP256-VRC26 from a third. These V1V2-directed bNAbs utilized strand-strand interactions between a protruding antibody loop and a V1V2 str...

  11. Polymorphism Trp64Arg of beta 3 adrenoreceptor gene: allelic frequencies and influence on insulin resistance in a multicenter study of Castilla-León Polimorfismo TRP64ARG del gen receptor beta 3: frecuencia alélica e influencia en la resistencia a la insulina en un estudio multicéntrico de Castilla y León

    Directory of Open Access Journals (Sweden)

    D. A. de Luis

    2010-04-01

    Full Text Available Background and objective: The genetic variant (Trp64Arg is a missense mutation located within the beta3 adrenoreceptor (Beta3AR. The aim of our study was to investigate the influence of Trp64Arg polymorphism in the Beta3AR gene on insulin resistance in obese patients and the allelic distribution of this polymorphismin a geographic area of Spain. Design: A population of 264 obese patients was analyzed. A bioimpedance, blood pressure, an assessment of nutritional intake, and biochemical parameters were measured. The beta 3 adrenoreceptor gene polymorphism(Trp64Arg was genotyped. Results: Two hundred and twenty six patients (77 males/149 females (85.6% had the genotype Trp64/Trp64 (wild type group with and average age of 41.12 ± 13.1 years and 38 patients (16 males/22 females Trp64/Arg64 (14.4% (mutant type group with an average age of 40.5 ± 12.7 years. High frequencies of Arg64 allele were observed in Salamanca and Valladolid. In the mutant type group, HOMA (3.75 ± 2.77 vs 5.27 ± 5.4; p Introducción y objetivos: La variante genética (Trp64Arg es una mutación localizada en el adrenoreceptor Beta 3 (Beta3AR. El objetivo de nuestro trabajo es evaluar la influencia de el polimorfismo Trp64Arg del gen de Beta3AR sobre la resistencia a la insulina en pacientes obesos, así como la distribución alélica de este polimorfismo en un área geográfica de España. Diseño: Una muestra de 264 pacientes obesos fue analizada. Se realizó una bioimpedancia, evaluación nutricional y análisis bioquímico. Se genotiparon a los pacientes en función delpolimorfismos Tr64Arg del gen adrenoreceptor-beta 3. Resultados: Un total de 227 pacientes (77 varones/149 mujeres (85,6% presentaron el genotipo Trp64/Trp64 (grupo genotipo salvaje, con una media de edad de 41,12 ± 13,1 años y un total de 38 pacientes (16 varones/22 mujeres Trp64/Arg64 (14,4% (grupo genotipo mutante con una edad media de 40,5 ± 12,7 años. Se detectó una alta frecuencia alélica (Arg64

  12. Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120

    Directory of Open Access Journals (Sweden)

    Wilson Ian A

    2006-07-01

    Full Text Available Abstract During natural HIV infection, an array of host receptors are thought to influence virus attachment and the kinetics of infection. In this study, to probe the interactions of HIV envelope (Env with various receptors, we assessed the inhibitory properties of various anti-Env monoclonal antibodies (mAbs in binding assays. To assist in detecting Env in attachment assays, we generated Fc fusions of full-length wild-type gp120 and several variable loop-deleted gp120s. Through investigation of the inhibition of Env binding to cell lines expressing CD4, CCR5, DC-SIGN, syndecans or combinations thereof, we found that the broadly neutralizing mAb, 2G12, directed to a unique carbohydrate epitope of gp120, inhibited Env-CCR5 binding, partially inhibited Env-DC-SIGN binding, but had no effect on Env-syndecan association. Furthermore, 2G12 inhibited Env attachment to primary monocyte-derived dendritic cells, that expressed CD4 and CCR5 primary HIV receptors, as well as DC-SIGN, and suggested that the dual activities of 2G12 could be valuable in vivo for inhibiting initial virus dissemination and propagation.

  13. Distributed Generation Market Demand Model (dGen): Documentation

    Energy Technology Data Exchange (ETDEWEB)

    Sigrin, Benjamin [National Renewable Energy Lab. (NREL), Golden, CO (United States); Gleason, Michael [National Renewable Energy Lab. (NREL), Golden, CO (United States); Preus, Robert [National Renewable Energy Lab. (NREL), Golden, CO (United States); Baring-Gould, Ian [National Renewable Energy Lab. (NREL), Golden, CO (United States); Margolis, Robert [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-02-01

    The Distributed Generation Market Demand model (dGen) is a geospatially rich, bottom-up, market-penetration model that simulates the potential adoption of distributed energy resources (DERs) for residential, commercial, and industrial entities in the continental United States through 2050. The National Renewable Energy Laboratory (NREL) developed dGen to analyze the key factors that will affect future market demand for distributed solar, wind, storage, and other DER technologies in the United States. The new model builds off, extends, and replaces NREL's SolarDS model (Denholm et al. 2009a), which simulates the market penetration of distributed PV only. Unlike the SolarDS model, dGen can model various DER technologies under one platform--it currently can simulate the adoption of distributed solar (the dSolar module) and distributed wind (the dWind module) and link with the ReEDS capacity expansion model (Appendix C). The underlying algorithms and datasets in dGen, which improve the representation of customer decision making as well as the spatial resolution of analyses (Figure ES-1), also are improvements over SolarDS.

  14. Variabilidade genética de caracteres forrageiros em Paspalum

    Directory of Open Access Journals (Sweden)

    Emerson André Pereira

    2012-10-01

    Full Text Available O objetivo deste trabalho foi determinar a variabilidade genética e a expressão de caracteres de interesse forrageiro em espécies de Paspalum. Os experimentos foram conduzidos em diferentes locais e anos de cultivo, em delineamento de blocos ao acaso, com três repetições. Foram avaliados cinco acessos de P. nicorae e dois de P. guenoarum, além da cultivar Pensacola (P. notatum, utilizada como testemunha. Foram quantificados os seguintes caracteres: relação folha/colmo, índice de colheita e massa de matéria seca total, de folhas e de colmo. Tanto os efeitos principais (genótipos, anos e locais de cultivo quanto a interação entre os fatores tiveram influência significativa sobre os caracteres avaliados. Os acessos avaliados apresentam variabilidade genética em caracteres de interesse forrageiro, bem como desempenho variável de acordo com o local e o ano de cultivo. A produção de matéria seca total e de folhas são os caracteres que mais contribuem para a detecção da variabilidade genética observada, independentemente do ano de avaliação.

  15. Estructura y diversidad genética en vacas Holstein de Antioquia usando un polimorfismo del gen bGH

    Directory of Open Access Journals (Sweden)

    Juan Rincon F.

    2013-03-01

    Full Text Available Objetivo. Determinar las frecuencias alélicas y genotípicas del polimorfismo del intrón 3 del gen bGH y estimar algunos parámetros de estructura poblacional en ganado Holstein. Materiales y métodos. El estudio se realizó con 1366 vacas Holstein en 120 hatos de 11 municipios del departamento de Antioquia. Se extrajo DNA por el método de Salting out y la genotipificación se realizó usando la técnica de PCR-RFLPs. La diversidad genética se determinó mediante la comparación de las heterocigosidades, El equilibrio de Hardy-Weinberg (HW y la diferenciación genética entre las poblaciones se realizó usando el software Arlequín 2.0 Las frecuencias alélicas y genotípicas se evaluaron mediante el paquete estadístico SAS®. Resultados. Las frecuencias genotípicas encontradas fueron 0.764 (+/+, 0.223 (+/- y 0.013 (-/- y las frecuencias alélicas 0.876 (+ y 0.124 (-. No se encontraron desviaciones del Equilibrio de Hardy Weinberg en ninguna de las subpoblaciones. La diversidad genética determinada mediante la comparación de las heterocigosidades fue relativamente baja entre poblaciones pero al interior de estas no. El valor de FST de toda la población fue de 0.0068 y significativo (p<0.05, algunos FST pareados también lo fueron, tomando valores desde 0.0 a 0.13. Los estadísticos FIT y FIS no fueron significativos. Conclusiones. El gen bGH es un candidato interesante para evaluar características de importancia económica ya que no parece haber sido sometido a selección directa, presenta una variabilidad media en las poblaciones, observándose diferenciación genética significativa entre distintos municipios, producto de los diferentes sistemas de producción y acceso a las biotecnologías.

  16. FutureGen Project Report

    Energy Technology Data Exchange (ETDEWEB)

    Cabe, Jim; Elliott, Mike

    2010-09-30

    This report summarizes the comprehensive siting, permitting, engineering, design, and costing activities completed by the FutureGen Industrial Alliance, the Department of Energy, and associated supporting subcontractors to develop a first of a kind near zero emissions integrated gasification combined cycle power plant and carbon capture and storage project (IGCC-CCS). With the goal to design, build, and reliably operate the first IGCC-CCS facility, FutureGen would have been the lowest emitting pulverized coal power plant in the world, while providing a timely and relevant basis for coal combustion power plants deploying carbon capture in the future. The content of this report summarizes key findings and results of applicable project evaluations; modeling, design, and engineering assessments; cost estimate reports; and schedule and risk mitigation from initiation of the FutureGen project through final flow sheet analyses including capital and operating reports completed under DOE award DE-FE0000587. This project report necessarily builds upon previously completed siting, design, and development work executed under DOE award DE-FC26- 06NT4207 which included the siting process; environmental permitting, compliance, and mitigation under the National Environmental Policy Act; and development of conceptual and design basis documentation for the FutureGen plant. For completeness, the report includes as attachments the siting and design basis documents, as well as the source documentation for the following: • Site evaluation and selection process and environmental characterization • Underground Injection Control (UIC) Permit Application including well design and subsurface modeling • FutureGen IGCC-CCS Design Basis Document • Process evaluations and technology selection via Illinois Clean Coal Review Board Technical Report • Process flow diagrams and heat/material balance for slurry-fed gasifier configuration • Process flow diagrams and heat/material balance

  17. Algoritmos genéticos locales

    OpenAIRE

    García-Martínez, Carlos; Lozano, Manuel

    2007-01-01

    Los Algoritmos Genéticos Locales son procedimientos que iterativamente re nan soluciones dadas. Su diferencia con procedimientos de mejora iterativa clásicos reside en el uso de operadores genéticos para realizar el re namiento. En este estudio presentamos un nuevo Algoritmo Genético Local Binario basado en un Algoritmo Genético Estacionario. Hemos comparado el Algoritmo Genético Local Binario con otros procedimientos de mejora iterativa de la literatura. Los res...

  18. Specifically modified Env immunogens activate B-cell precursors of broadly neutralizing HIV-1 antibodies in transgenic mice.

    Science.gov (United States)

    McGuire, Andrew T; Gray, Matthew D; Dosenovic, Pia; Gitlin, Alexander D; Freund, Natalia T; Petersen, John; Correnti, Colin; Johnsen, William; Kegel, Robert; Stuart, Andrew B; Glenn, Jolene; Seaman, Michael S; Schief, William R; Strong, Roland K; Nussenzweig, Michel C; Stamatatos, Leonidas

    2016-01-01

    VRC01-class broadly neutralizing HIV-1 antibodies protect animals from experimental infection and could contribute to an effective vaccine response. Their predicted germline forms (gl) bind Env inefficiently, which may explain why they are not elicited by HIV-1 Env-immunization. Here we show that an optimized Env immunogen can engage multiple glVRC01-class antibodies. Furthermore, this immunogen activates naive B cells expressing the human germline heavy chain of 3BNC60, paired with endogenous mouse light chains in vivo. To address whether it activates B cells expressing the fully humanized gl3BNC60 B-cell receptor (BCR), we immunized mice carrying both the heavy and light chains of gl3BNC60. B cells expressing this BCR display an autoreactive phenotype and fail to respond efficiently to soluble forms of the optimized immunogen, unless it is highly multimerized. Thus, specifically designed Env immunogens can activate naive B cells expressing human BCRs corresponding to precursors of broadly neutralizing HIV-1 antibodies even when the B cells display an autoreactive phenotype. PMID:26907590

  19. Construção de um env quimérico HIV-1 / HIV-2

    OpenAIRE

    Figueiredo, Inês Brito

    2014-01-01

    Dissertação para obtenção do grau de Mestre no Instituto Superior de Ciências da Saúde Egas Moniz Introdução: O Síndrome da Imunodeficiência Adquirida (SIDA) representa, a nível mundial, um dos principais problemas de saúde pública. O agente etiológico responsável por esta pandemia é o Vírus da Imunodeficiência Humana (VIH), que afeta 35 milhões de pessoas em todo o mundo. Objetivos: Construção dum env quimérico contendo a região C2, V3 e C3 do VIH-2 e as restantes do VIH-1 e reconstruç...

  20. Cell cycle checkpoints in Caenorhabditis elegans: the 14-3-3 gene par-5 is required for germline development and DNA damage response / Checkpoints del ciclo celular en Caenorhabditis elegans: el gen 14-3-3, par-5, es necesario para el desarrollo y respuesta al daño genómico de la línea germinal

    OpenAIRE

    Aristizábal Corrales, David

    2012-01-01

    [spa] Las proteínas 14-3-3 han sido ampliamente estudiadas desde levadura hasta mamíferos y han sido asociadas con múltiples roles en procesos como ciclo celular, apoptosis y la respuesta al estrés. Así mismo estas proteínas se han visto involucradas en enfermedades neurodegenerativas y cáncer. De hecho, las proteínas 14-3-3 han sido propuestas como posibles agentes terapéuticos en el tratamiento contra el cáncer. En mamíferos existen 7 genes que codifican para proteínas 14-3-3, mientras en C...

  1. GenLab, Laboratorio Virtual de Genética

    Directory of Open Access Journals (Sweden)

    García Sergio

    2000-12-01

    Full Text Available

    GenLab es el nombre que tiene el software diseñado por nosotros, en el cual se modela el proceso meiótico y la fecundación en organismos diploides. El objetivo de esta aplicación es ilustrar el resultado de un cruce determinado, tratando de ser lo más ajustados a la realidad. La modelación de la reproducción sexual se realiza internamente y el GenLab se limita a presentar los resultados según el número de descendencia seleccionado para un cruce específico, esto significa que se puede escoger una gran cantidad de características para los parentales y se puede estudiar la frecuencia de estos en la descendencia. El modelo cuenta con base de datos donde están almacenados algunos de los locus de Drosophila melanogaster junto con su ubicación en centimorgans 1. EI propósito de este modelo es servir como herramienta pedagógica  y didáctica tanto en universidades como en colegios, facilitando el aprendizaje de algunos principios básicos de la genética, por lo cual puede ser usado si se cuenta con una conexión a Internet y un navegador visitando http://biologia.unal.edu.co/fidel.

  2. Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend.

    Science.gov (United States)

    Stackebrandt, E; Koch, C; Gvozdiak, O; Schumann, P

    1995-10-01

    The results of a phylogenetic and chemotaxonomic analysis of the genus Micrococcus indicated that it is significantly heterogeneous. Except for Micrococcus lylae, no species groups phylogenetically with the type species of the genus, Micrococcus luteus. The other members of the genus form three separate phylogenetic lines which on the basis of chemotaxonomic properties can be assigned to four genera. These genera are the genus Kocuria gen. nov. for Micrococcus roseus, Micrococcus varians, and Micrococcus kristinae, described as Kocuria rosea comb. nov., Kocuria varians comb. nov., and Kocuria kristinae comb. nov., respectively; the genus Nesterenkonia gen. nov. for Micrococcus halobius, described as Nesterenkonia halobia comb. nov.; the genus Nesterenkonia gen. nov. for Micrococcus halobius, described as Nesterenkonia halobia comb. nov.; the genus Dermacoccus gen. nov. for Micrococcus nishinomiyaensis, described as Dermacoccus nishinomiyaensis comb. nov.; and the genus Kytocossus gen. nov. for Micrococcus sedentarius, described as Kytococcus sedentarius comb. nov. M. luteus and M. lylae, which are closely related phylogenetically but differ in some chemotaxonomic properties, are the only species that remain in the genus Micrococcus Cohn 1872. An emended description of the genus Micrococcus is given [corrected]. PMID:7547287

  3. Macrophage entry mediated by HIV Envs from brain and lymphoid tissues is determined by the capacity to use low CD4 levels and overall efficiency of fusion

    International Nuclear Information System (INIS)

    HIV infects macrophages and microglia in the central nervous system (CNS), which express lower levels of CD4 than CD4+ T cells in peripheral blood. To investigate mechanisms of HIV neurotropism, full-length env genes were cloned from autopsy brain and lymphoid tissues from 4 AIDS patients with HIV-associated dementia (HAD). Characterization of 55 functional Env clones demonstrated that Envs with reduced dependence on CD4 for fusion and viral entry are more frequent in brain compared to lymphoid tissue. Envs that mediated efficient entry into macrophages were frequent in brain but were also present in lymphoid tissue. For most Envs, entry into macrophages correlated with overall fusion activity at all levels of CD4 and CCR5. gp160 nucleotide sequences were compartmentalized in brain versus lymphoid tissue within each patient. Proline at position 308 in the V3 loop of gp120 was associated with brain compartmentalization in 3 patients, but mutagenesis studies suggested that P308 alone does not contribute to reduced CD4 dependence or macrophage-tropism. These results suggest that HIV adaptation to replicate in the CNS selects for Envs with reduced CD4 dependence and increased fusion activity. Macrophage-tropic Envs are frequent in brain but are also present in lymphoid tissues of AIDS patients with HAD, and entry into macrophages in the CNS and other tissues is dependent on the ability to use low receptor levels and overall efficiency of fusion

  4. Mutagenesis of tyrosine and di-leucine motifs in the HIV-1 envelope cytoplasmic domain results in a loss of Env-mediated fusion and infectivity

    Directory of Open Access Journals (Sweden)

    Claiborne Daniel T

    2011-05-01

    Full Text Available Abstract Background The gp41 component of the Human Immunodeficiency Virus (HIV envelope glycoprotein (Env contains a long cytoplasmic domain (CD with multiple highly conserved tyrosine (Y and dileucine (LL motifs. Studies suggest that the motifs distal to major endocytosis motif (Y712HRL, located at residues 712-715 of Env, may contribute to Env functionality in the viral life cycle. In order to examine the biological contribution of these motifs in the biosynthesis, transport, and function of Env, we constructed two panels of mutants in which the conserved Y- and LL-motifs were sequentially substituted by alternative residues, either in the presence or absence of Y712. Additional mutants targeting individual motifs were then constructed. Results All mutant Envs, when expressed in the absence of other viral proteins, maintained at least WT levels of Env surface staining by multiple antibodies. The Y712 mutation (Y712C contributed to at least a 4-fold increase in surface expression for all mutants containing this change. Sequential mutagenesis of the Y- and LL-motifs resulted in a generally progressive decrease in Env fusogenicity. However, additive mutation of dileucine and tyrosine motifs beyond the tyrosine at residue 768 resulted in the most dramatic effects on Env incorporation into virions, viral infectivity, and virus fusion with target cells. Conclusions From the studies reported here, we show that mutations of the Y- and LL-motifs, which effectively eliminate the amphipathic nature of the lytic peptide 2 (LLP2 domain or disrupt YW and LL motifs in a region spanning residues 795-803 (YWWNLLQYW, just C-terminal of LLP2, can dramatically interfere with biological functions of HIV-1 Env and abrogate virus replication. Because these mutant proteins are expressed at the cell surface, we conclude that tyrosine and di-leucine residues within the cytoplasmic domain of gp41 play critical roles in HIV-1 replication that are distinct from that of

  5. Le goût de Genève

    OpenAIRE

    Lévy, Bertrand

    2006-01-01

    Le livre contient une anthologie de textes littéraires sur Genève, avec 5 sections : L'esprit de Genève, voir Genève, goûter Genève, vivre à Genève, annexes (renseignements pratiques et bibliographie)

  6. A rev1-vpu polymorphism unique to HIV-1 subtype A and C strains impairs envelope glycoprotein expression from rev-vpu-env cassettes and reduces virion infectivity in pseudotyping assays

    International Nuclear Information System (INIS)

    Functional studies of HIV-1 envelope glycoproteins (Envs) commonly include the generation of pseudoviruses, which are produced by co-transfection of rev-vpu-env cassettes with an env-deficient provirus. Here, we describe six Env constructs from transmitted/founder HIV-1 that were defective in the pseudotyping assay, although two produced infectious virions when expressed from their cognate proviruses. All of these constructs exhibited an unusual gene arrangement in which the first exon of rev (rev1) and vpu were in the same reading frame without an intervening stop codon. Disruption of the rev1-vpu fusion gene by frameshift mutation, stop codon, or abrogation of the rev initiation codon restored pseudovirion infectivity. Introduction of the fusion gene into wildtype Env cassettes severely compromised their function. The defect was not due to altered env and rev transcription or a dominant negative effect of the expressed fusion protein, but seemed to be caused by inefficient translation at the env initiation codon. Although the rev1-vpu polymorphism affects Env expression only in vitro, it can cause problems in studies requiring Env complementation, such as analyses of co-receptor usage and neutralization properties, since 3% of subtype A, 20% of subtype C and 5% of CRF01A/E viruses encode the fusion gene. A solution is to eliminate the rev initiation codon when amplifying rev-vpu-env cassettes since this increases Env expression irrespective of the presence of the polymorphism.

  7. Algoritmos genéticos incrementales

    OpenAIRE

    Nesmachnow, Sergio; Dominioni, Federico; Musso, Pablo

    2005-01-01

    Este artículo presenta el estudio, diseño e implementación de un modelo particular de algoritmos genéticos paralelos, los algoritmos genéticos incrementales. La principal ventaja del motor de algoritmos genéticos implementado es su potencialidad de ejecución en entornos no dedicados, detectando la carga generada por los usuarios del entorno y consumiendo de un modo “inteligente” los recursos computacionales disponibles. Se presenta la descripción teórica del modelo de algoritmos genéticos inc...

  8. Comportamento de dois genótipos de milho cultivados em sistema de aléias preestabelecido com diferentes leguminosas arbóreas Behaviour of two maize genotypes grown in alley cropping system pre-established with diferents leguminous trees

    Directory of Open Access Journals (Sweden)

    Andréia Araújo Lima Leite

    2008-12-01

    Full Text Available O cultivo em aléias tem sido recomendado como alternativa para a substituição da agricultura de corte e queima, no trópico úmido, devido à grande capacidade de produção de matéria orgânica e de reciclagem de nutrientes, mas algumas dúvidas quanto à sustentabilidade e à competição interespecífica são persistentes. O objetivo no trabalho foi avaliar a viabilidade da cultura do milho em um sistema de cultivo em aléias de leguminosas arbóreas. O delineamento experimental utilizado foi em blocos casualisados, com quatro repetições dos tratamentos: aléias de sombreiro (Clitoria fairchildiana, ingá (Inga edulis, guandu (Cajanus cajan e leucena (Leucaena leucocephala e uma testemunha sem aléias. Foram avaliadas a remobilização de carbono e nitrogênio, massa de grãos, massa de mil grãos e competição interespecífica entre as cultivares de milho e as leguminosas. A produção de grãos foi maior nas parcelas com C. fairchildiana e L. leucocephala. A produtividade do híbrido de milho foi superior à da variedade em todos os tratamentos. A produtividade e a massa de mil grãos de milho não são negativamente afetadas pela distância da linha da leguminosa arbórea. Esse estudo conclui que o sistema de aléias com leguminosas arbóreas é uma alternativa importante ao manejo sustentável dos agroecossistemas no tropico úmido. Além disso, nessa região a produtividade em grãos na cultura do milho é favorecida no sistema de aléias preeestabelecidas com as leguminosas arbóreas sombreiro, ingá e leucena e pela utilização de genótipos eficientes no aproveitamento do nitrogênio, cujo sincronismo entre a liberação e a absorção do N aplicado por meio das leguminosas deve ser aprimorado.Alley cropping has been recommended as alternative land use to slash-and-burn agriculture in humid tropics. However, interespecific competition between cash crop and hedgerow can reduce this potential. This study aimed to evaluate the

  9. Atencion integral al paciente con poliquistosis renal genética: Perfil clínico y experiencia vital subjetiva Integral care of patient with genetic renal polycystosis: Clinical profile and subject vital experience

    Directory of Open Access Journals (Sweden)

    M Calderó Urrea

    2007-09-01

    Full Text Available La poliquistosis renal autosómica dominante afecta al 5%-10% de personas en programa de hemodiálisis o de trasplante renal. Este estudio tiene un doble objetivo: conocer el perfil clínico del paciente con poliquistosis renal y comprender cómo esta enfermedad influye en las diferentes etapas vitales. El diseño de investigación es cuantitativo y cualitativo para dar respuesta a cada uno de los objetivos propuestos. La perspectiva cuantitativa abarca un estudio descriptivo retrospectivo de revisión de las historias clínicas de los pacientes atendidos en nuestro centro con este diagnóstico entre los años 2000 y 2005 (N=161. Para abarcar aspectos cualitativos de la enfermedad se han realizado dos entrevistas en profundidad, semiestructuradas, a personas cuyo diagnóstico ha actuado como condicionante en la toma de decisiones personales y familiares. El perfil de paciente con poliquistosis renal es el de una persona de 58 años de media con poliquistosis hepática, así como antecedentes familiares de la enfermedad y diversas patologías asociadas, siendo la más frecuente la hipertensión arterial. Las entrevistas en profundidad denotan la presencia de dolor crónico, con una alteración de la vida cotidiana y de la dinámica familiar. La incerteza en cuanto a su evolución y tratamiento produce ansiedad y un desgaste emocional progresivo.Autosomic dominant renal polycystosis affects 5%-10% of people on haemodialysis or with kidney transplants. This study has a two-fold purpose: to determine the clinical profile of the patient with renal polycystosis and to understand how this dise ase affects different vital stages. The research design is quantitative and qualitative in order to meet each of the proposed goals. The quantitative perspective encompasses a retrospective descriptive review of the case histories of the patients treated at our centre with this diagnosis between the years 2000 and 2005 (N=161. To cover qualitative aspects of the

  10. Frecuencias alélicas y fenotípicas de los marcadores genéticos enzimáticos: Fosfoglucomutasa 1 (FGM 1, Esterasa D (EsD y Fosfatasa ácida eritrocitaria (FAE en población residente en la ciudad de Medellín, 1996-1997

    Directory of Open Access Journals (Sweden)

    María Victoria Hurtado Ángel

    2000-01-01

    Full Text Available Este estudio descriptivo de las enzimas fosfoglucomutasa 1 (fgm 1, esterasa D (EsD y Fosfatasa ácida eritrocitaria (FAE como marcadores genéticos polimórficos, se realizó en 145 muestras de sangre, tomadas a igual númerode donantes de los tres principales bancos de sangre de la ciudad, por el método de electroforesis convencional, con sistema de refrigeración, en gel de agarosa. La probabilidad de identificación para cada uno de los marcadores fue buena; la mejor fue la subtipificación de la FGM1, seguida de la FAE por ser las más polimórficas. Igual resultado se obtuvo para el poder de discriminación. Las frecuencias alélicas y fenotípicas se encontraron de manera representativa en la población, en la que estaban presentes 21 de los 22 fenotipos posibles. Los resultados obtenidos muestran que los tres marcadores enzimáticos estudiados se encuentran en equilibrio de Hardy-Weinberg, lo cual se comprobó aplicando el método estadístico 2 y la corrección de Bonferroni para la FGM 1 subtipo; por ello son de gran utilidad para cálculos de probabilidad en casos forenses con población residente en Antioquia, pues se encontró que dada la procedencia de los donantes y sus padres se puede hacer inferencia a todo el departamento.

  11. Justicia en salud y genética

    Directory of Open Access Journals (Sweden)

    Maria Graciela De Ortuzar

    2014-06-01

    Full Text Available Las expectativas puestas en el conocimiento genético exceden el ámbito de la medicina tradiciona, debido a que la intervención directa en la lotería natural demandaría el replanteamiento de conceptos centrales de justicia en salud: necesidades médicas, enfermedad, normalidad, e igualdad de oportunidades en el acceso a la salud. El punto en debate es sí el replanteo de dichos conceptos conlleva un cambio radical en las teorías de justicia (libertariana y/o liberal, mostrando su obsolescencia, o sí simplemente se requiere ampliar dichos conceptos claves por fallas estructurales en las mismas teorías. Como hipótesis general considero que los supuestos cuestionamientos, lejos de socavar las bases de las teorías de justicia, sólo ponen en evidencia sus viejos problemas estructurales. Por razones expositivas, dividiré la presentación tres partes. En la Primera parte, analizo la teoría libertariana, estudiando las contradicciones del modelo a través del impacto de la información genética en el seguro privado de salud. En la Segunda Parte, desarrollo la propuesta alternativa liberal rawlsianadanielsiana del modelo de seguro público, evaluando las implicaciones de la genética a partir de la crítica de su concepto biológico de enfermedad y su restricción al acceso a la salud por necesidades naturales. En la Tercera parte presento un modelo integral de necesidades y capacidades básicas, comprendiendo la prevención, el tratamiento y el mejoramiento moralmente permisible (genético y no genético.Mi aporte principal consiste en la elaboración de este modelo normativo integral de necesidades y capacidades para la regulación conjunta de la información y terapia genética con los restantes problemas de salud.

  12. SHIV-1157i and passaged progeny viruses encoding R5 HIV-1 clade C env cause AIDS in rhesus monkeys

    OpenAIRE

    Siddappa Nagadenahalli B; Kramer Victor G; Chenine Agnès L; Sharma Prachi; Ong Helena; Song Ruijiang; Rasmussen Robert A; Humbert Michael; Xu Weidong; Else James G; Novembre Francis J; Strobert Elizabeth; O'Neil Shawn P; Ruprecht Ruth M

    2008-01-01

    Abstract Background Infection of nonhuman primates with simian immunodeficiency virus (SIV) or chimeric simian-human immunodeficiency virus (SHIV) strains is widely used to study lentiviral pathogenesis, antiviral immunity and the efficacy of AIDS vaccine candidates. SHIV challenges allow assessment of anti-HIV-1 envelope responses in primates. As such, SHIVs should mimic natural HIV-1 infection in humans and, to address the pandemic, encode HIV-1 Env components representing major viral subty...

  13. Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques.

    Science.gov (United States)

    Vargas-Inchaustegui, Diego A; Demers, Andrew; Shaw, Julia M; Kang, Guobin; Ball, David; Tuero, Iskra; Musich, Thomas; Mohanram, Venkatramanan; Demberg, Thorsten; Karpova, Tatiana S; Li, Qingsheng; Robert-Guroff, Marjorie

    2016-02-15

    Measurement of Ag-specific T follicular helper (TFH) cell activity in rhesus macaques has not previously been reported. Given that rhesus macaques are the animal model of choice for evaluating protective efficacy of HIV/SIV vaccine candidates and that TFH cells play a pivotal role in aiding B cell maturation, quantifying vaccine induction of HIV/SIV-specific TFH cells would greatly benefit vaccine development. In this study, we quantified SIV Env-specific IL-21-producing TFH cells for the first time, to our knowledge, in a nonhuman primate vaccine study. Macaques were primed twice mucosally with adenovirus 5 host range mutant recombinants encoding SIV Env, Rev, Gag, and Nef followed by two i.m. boosts with monomeric SIV gp120 or oligomeric SIV gp140 proteins. At 2 wk after the second protein boost, we obtained lymph node biopsy specimens and quantified the frequency of total and SIV Env-specific IL-21(+) TFH cells and total germinal center B cells, the size and number of germinal centers, and the frequency of SIV-specific Ab-secreting cells in B cell zones. Multiple correlation analyses established the importance of TFH for development of B cell responses in systemic and mucosally localized compartments, including blood, bone marrow, and rectum. Our results suggest that the SIV-specific TFH cells, initially induced by replicating adenovirus-recombinant priming, are long lived. The multiple correlations of SIV Env-specific TFH cells with systemic and mucosal SIV-specific B cell responses indicate that this cell population should be further investigated in HIV vaccine development as a novel correlate of immunity. PMID:26773147

  14. Timing of use of cod liver oil, a vitamin D source, and multiple sclerosis risk: The EnvIMS study.

    OpenAIRE

    Cortese, Marianna; Riise, Trond; Bjørnevik, Kjetil Lauvland; Holmøy, Trygve; Kampman, Margitta Theodora; Magalhaes, Sandra; Pugliatti, Maura; Wolfson, Christina; Myhr, Kjell-Morten

    2015-01-01

    Background: Low vitamin D levels have been associated with an increased risk of multiple sclerosis (MS), although it remains unknown whether this relationship varies by age. Objective: The objective of this paper is to investigate the association between vitamin D3 supplementation through cod liver oil at different postnatal ages and MS risk. Methods: In the Norwegian component of the multinational case-control study Environmental Factors In Multiple Sclerosis (EnvIMS), a total of 953 M...

  15. Cocirculation of Two env Molecular Variants, of Possible Recombinant Origin, in Gorilla and Chimpanzee Simian Foamy Virus Strains from Central Africa

    OpenAIRE

    Richard, Léa; Rua, Réjane; Betsem, Edouard; Mouinga-Ondémé, Augustin; Kazanji, Mirdad; Leroy, Eric; Njouom, Richard; Buseyne, Florence; Afonso, Philippe V; Gessain, Antoine

    2015-01-01

    Simian foamy virus (SFV) is a ubiquitous retrovirus in nonhuman primates (NHPs) that can be transmitted to humans, mostly through severe bites. In the past few years, our laboratory has identified more than 50 hunters from central Africa infected with zoonotic SFVs. Analysis of the complete sequences of five SFVs obtained from these individuals revealed that env was the most variable gene. Furthermore, recombinant SFV strains, some of which involve sequences in the env gene, were recently ide...

  16. Production of Mucosally Transmissible SHIV Challenge Stocks from HIV-1 Circulating Recombinant Form 01_AE env Sequences.

    Directory of Open Access Journals (Sweden)

    Lawrence J Tartaglia

    2016-02-01

    Full Text Available Simian-human immunodeficiency virus (SHIV challenge stocks are critical for preclinical testing of vaccines, antibodies, and other interventions aimed to prevent HIV-1. A major unmet need for the field has been the lack of a SHIV challenge stock expressing circulating recombinant form 01_AE (CRF01_AE env sequences. We therefore sought to develop mucosally transmissible SHIV challenge stocks containing HIV-1 CRF01_AE env derived from acutely HIV-1 infected individuals from Thailand. SHIV-AE6, SHIV-AE6RM, and SHIV-AE16 contained env sequences that were >99% identical to the original HIV-1 isolate and did not require in vivo passaging. These viruses exhibited CCR5 tropism and displayed a tier 2 neutralization phenotype. These challenge stocks efficiently infected rhesus monkeys by the intrarectal route, replicated to high levels during acute infection, and established chronic viremia in a subset of animals. SHIV-AE16 was titrated for use in single, high dose as well as repetitive, low dose intrarectal challenge studies. These SHIV challenge stocks should facilitate the preclinical evaluation of vaccines, monoclonal antibodies, and other interventions targeted at preventing HIV-1 CRF01_AE infection.

  17. Proviral amplification of the Gypsy endogenous retrovirus of Drosophila melanogaster involves env-independent invasion of the female germline.

    Science.gov (United States)

    Chalvet, F; Teysset, L; Terzian, C; Prud'homme, N; Santamaria, P; Bucheton, A; Pélisson, A

    1999-05-01

    Gypsy is an infectious endogenous retrovirus of Drosophila melanogaster. The gypsy proviruses replicate very efficiently in the genome of the progeny of females homozygous for permissive alleles of the flamenco gene. This replicative transposition is correlated with derepression of gypsy expression, specifically in the somatic cells of the ovaries of the permissive mothers. The determinism of this amplification was studied further by making chimeric mothers containing different permissive/restrictive and somatic/germinal lineages. We show here that the derepression of active proviruses in the permissive soma is necessary and sufficient to induce proviral insertions in the progeny, even if the F1 flies derive from restrictive germ cells devoid of active proviruses. Therefore, gypsy endogenous multiplication results from the transfer of some gypsy-encoded genetic material from the soma towards the germen of the mother and its subsequent insertion into the chromosomes of the progeny. This transfer, however, is not likely to result from retroviral infection of the germline. Indeed, we also show here that the insertion of a tagged gypsy element, mutant for the env gene, occurs at high frequency, independently of the production of gypsy Env proteins by any transcomplementing helper. The possible role of the env gene for horizontal transfer to new hosts is discussed. PMID:10228177

  18. Structures of HIV-1-Env V1V2 with broadly neutralizing antibodies reveal commonalities that enable vaccine design

    Science.gov (United States)

    Gorman, Jason; Soto, Cinque; Yang, Max M.; Davenport, Thaddeus M.; Guttman, Miklos; Bailer, Robert T.; Chambers, Michael; Chuang, Gwo-Yu; DeKosky, Brandon J.; Doria-Rose, Nicole A.; Druz, Aliaksandr; Ernandes, Michael J.; Georgiev, Ivelin S.; Jarosinski, Marissa C.; Joyce, M. Gordon; Lemmin, Thomas M.; Leung, Sherman; Louder, Mark K.; McDaniel, Jonathan R.; Narpala, Sandeep; Pancera, Marie; Stuckey, Jonathan; Wu, Xueling; Yang, Yongping; Zhang, Baoshan; Zhou, Tongqing; Mullikin, James C.; Baxa, Ulrich; Georgiou, George; McDermott, Adrian B.; Bonsignori, Mattia; Haynes, Barton F.; Moore, Penny L.; Morris, Lynn; Lee, Kelly K.; Shapiro, Lawrence; Mascola, John R.; Kwong, Peter D.

    2016-01-01

    Broadly neutralizing antibodies (bNAbs) against HIV-1-Env V1V2 arise in multiple donors. However, atomic-level interactions had only been determined with antibodies from a single donor, making commonalities in recognition uncertain. Here we report the co-crystal structure of V1V2 with antibody CH03 from a second donor and model Env interactions of antibody CAP256-VRC26 from a third. These V1V2-directed bNAbs utilized strand-strand interactions between a protruding antibody loop and a V1V2 strand, but differed in their N-glycan recognition. Ontogeny analysis indicated protruding loops to develop early, with glycan interactions maturing over time. Altogether, the multidonor information suggested V1V2-directed bNAbs to form an ‘extended class’, for which we engineered ontogeny-specific antigens: Env trimers with chimeric V1V2s that interacted with inferred ancestor and intermediate antibodies. The ontogeny-based design of vaccine antigens described here may provide a general means for eliciting antibodies of a desired class. PMID:26689967

  19. Environmental management in PowerGen

    International Nuclear Information System (INIS)

    PowerGen has developed a company wide Environmental Compliance Policy. This incorporates an environmental management system and sets down Minimum Management Standards. The Policy is supported by an environmental audit programme. PowerGen has participated in the development of standardised Environmental Management Systems within BS7750 and the European Community Eco - Management and Audit Scheme. 1 fig

  20. The conserved dileucine- and tyrosine-based motifs in MLV and MPMV envelope glycoproteins are both important to regulate a common Env intracellular trafficking

    Directory of Open Access Journals (Sweden)

    Lopez-Vergès Sandra

    2006-09-01

    Full Text Available Abstract Background Retrovirus particles emerge from the assembly of two structural protein components, Gag that is translated as a soluble protein in the cytoplasm of the host cells, and Env, a type I transmembrane protein. Because both components are translated in different intracellular compartments, elucidating the mechanisms of retrovirus assembly thus requires the study of their intracellular trafficking. Results We used a CD25 (Tac chimera-based approach to study the trafficking of Moloney murine leukemia virus and Mason-Pfizer monkey virus Env proteins. We found that the cytoplasmic tails (CTs of both Env conserved two major signals that control a complex intracellular trafficking. A dileucine-based motif controls the sorting of the chimeras from the trans-Golgi network (TGN toward endosomal compartments. Env proteins then follow a retrograde transport to the TGN due to the action of a tyrosine-based motif. Mutation of either motif induces the mis-localization of the chimeric proteins and both motifs are found to mediate interactions of the viral CTs with clathrin adaptors. Conclusion This data reveals the unexpected complexity of the intracellular trafficking of retrovirus Env proteins that cycle between the TGN and endosomes. Given that Gag proteins hijack endosomal host proteins, our work suggests that the endosomal pathway may be used by retroviruses to ensure proper encountering of viral structural Gag and Env proteins in cells, an essential step of virus assembly.

  1. Human Ubc9 is involved in intracellular HIV-1 Env stability after trafficking out of the trans-Golgi network in a Gag dependent manner.

    Directory of Open Access Journals (Sweden)

    Christopher R Bohl

    Full Text Available The cellular E2 Sumo conjugase, Ubc9 interacts with HIV-1 Gag, and is important for the assembly of infectious HIV-1 virions. In the previous study we demonstrated that in the absence of Ubc9, a defect in virion assembly was associated with decreased levels of mature intracellular Envelope (Env that affected Env incorporation into virions and virion infectivity. We have further characterized the effect of Ubc9 knockdown on HIV Env processing and assembly. We found that gp160 stability in the endoplasmic reticulum (ER and its trafficking to the trans-Golgi network (TGN were unaffected, indicating that the decreased intracellular mature Env levels in Ubc9-depleted cells were due to a selective degradation of mature Env gp120 after cleavage from gp160 and trafficked out of the TGN. Decreased levels of Gag and mature Env were found to be associated with the plasma membrane and lipid rafts, which suggest that these viral proteins were not trafficked correctly to the assembly site. Intracellular gp120 were partially rescued when treated with a combination of lysosome inhibitors. Taken together our results suggest that in the absence of Ubc9, gp120 is preferentially degraded in the lysosomes likely before trafficking to assembly sites leading to the production of defective virions. This study provides further insight in the processing and packaging of the HIV-1 gp120 into mature HIV-1 virions.

  2. Genética del asma Genetics of asthma

    Directory of Open Access Journals (Sweden)

    S. de Arriba Méndez

    2010-01-01

    Full Text Available El asma tiene una clara agregación familiar, siendo más frecuente el desarrollo de asma en un niño si sus padres son asmáticos; asimismo, es mayor la concordancia en los gemelos idénticos y son numerosos los estudios epidemiológicos que han destacado que los antecedentes familiares constituyen un importante factor de riesgo para desarrollar asma. Diferentes estudios con gemelos han concluido que la herencia del asma está en torno al 60%, destacando por tanto la importancia de dichos factores genéticos...

  3. Frigitilla gen. nov., a new genus of Amazonian Mutillidae (Hymenoptera).

    Science.gov (United States)

    Bartholomay, Pedro R; Williams, Kevin A; Luz, David R; Morato, Elder F

    2015-01-01

    Mutilla frigidula Cresson, 1902 was transferred to Tobantilla by Williams et al. (2011), based on morphological similarities with females of that genus. Discovery of the male of this species indicated significant morphological differences from Tobantilla. We therefore erect the genus, Frigitilla gen. nov., for Mutilla frigidula. Herein, we describe the male of F. frigidula (Cresson, 1902), comb. nov., associate it with its host (Trypoxylon spp.), and discuss its relations to other mutillid genera. The impact of collecting method and specimen age on the integumental coloration of specimens is discussed, emphasizing the need to diagnose species on consistent structural features rather than differences in color pattern. PMID:26249052

  4. Genève Reconnaissante

    CERN Multimedia

    2001-01-01

    Robert Cailliau (centre), with Geneva's Mayor Alain Vaissade (left) and Jean Erhardt, Secretary General of the Administrative Council of Geneva (right). Geneva recognised the contribution of two CERN people to the reputation of the city last Tuesday when Mayor Alain Vaissade presented the Genève Reconaissante Medal to Tim Berners-Lee and Robert Cailliau. Berners-Lee, who was not able to be present in person, invented the World Wide Web at CERN just over a decade ago, while Cailliau was his first collaborator. Quoting Cailliau, Vaissade said that whilst there is no doubt that something like the Web would have appeared sooner or later, the fact that it happened at CERN, in Geneva, was no accident. Both the Laboratory and the city are places where people from around the world meet and work in harmony.

  5. Marcadores genéticos relacionados con el déficit cognitivo en el envejecimiento

    OpenAIRE

    Solé-Padullés, Cristina; Clemente, Imma C.; Bartrés Faz, David

    2004-01-01

    En el presente trabajo se revisan estudios realizados en relación a los marcadores genéticos de vulnerabilidad al deterioro cognitivo en el envejecimiento. En concreto, se estudia el papel de varios polimorfismos genéticos en las entidades de Alteración de la Memoria Asociada a la Edad (AAMI, del inglés Age Associated Memory Impairment) y Alteración Cognitiva Leve (MCI, del inglés Mild Cognitive Impairment), por ser condiciones de alto riesgo para el desarrollo ...

  6. An automated HIV-1 Env-pseudotyped virus production for global HIV vaccine trials.

    Directory of Open Access Journals (Sweden)

    Anke Schultz

    Full Text Available BACKGROUND: Infections with HIV still represent a major human health problem worldwide and a vaccine is the only long-term option to fight efficiently against this virus. Standardized assessments of HIV-specific immune responses in vaccine trials are essential for prioritizing vaccine candidates in preclinical and clinical stages of development. With respect to neutralizing antibodies, assays with HIV-1 Env-pseudotyped viruses are a high priority. To cover the increasing demands of HIV pseudoviruses, a complete cell culture and transfection automation system has been developed. METHODOLOGY/PRINCIPAL FINDINGS: The automation system for HIV pseudovirus production comprises a modified Tecan-based Cellerity system. It covers an area of 5×3 meters and includes a robot platform, a cell counting machine, a CO(2 incubator for cell cultivation and a media refrigerator. The processes for cell handling, transfection and pseudovirus production have been implemented according to manual standard operating procedures and are controlled and scheduled autonomously by the system. The system is housed in a biosafety level II cabinet that guarantees protection of personnel, environment and the product. HIV pseudovirus stocks in a scale from 140 ml to 1000 ml have been produced on the automated system. Parallel manual production of HIV pseudoviruses and comparisons (bridging assays confirmed that the automated produced pseudoviruses were of equivalent quality as those produced manually. In addition, the automated method was fully validated according to Good Clinical Laboratory Practice (GCLP guidelines, including the validation parameters accuracy, precision, robustness and specificity. CONCLUSIONS: An automated HIV pseudovirus production system has been successfully established. It allows the high quality production of HIV pseudoviruses under GCLP conditions. In its present form, the installed module enables the production of 1000 ml of virus-containing cell

  7. Disulfide Sensitivity in the Env Protein Underlies Lytic Inactivation of HIV-1 by Peptide Triazole Thiols.

    Science.gov (United States)

    Bailey, Lauren D; Kalyana Sundaram, Ramalingam Venkat; Li, Huiyuan; Duffy, Caitlin; Aneja, Rachna; Rosemary Bastian, Arangassery; Holmes, Andrew P; Kamanna, Kantharaju; Rashad, Adel A; Chaiken, Irwin

    2015-12-18

    We investigated the mode of action underlying lytic inactivation of HIV-1 virions by peptide triazole thiol (PTT), in particular the relationship between gp120 disulfides and the C-terminal cysteine-SH required for virolysis. Obligate PTT dimer obtained by PTT SH cross-linking and PTTs with serially truncated linkers between pharmacophore isoleucine-ferrocenyltriazole-proline-tryptophan and cysteine-SH were synthesized. PTT variants showed loss of lytic activity but not binding and infection inhibition upon SH blockade. A disproportionate loss of lysis activity vs binding and infection inhibition was observed upon linker truncation. Molecular docking of PTT onto gp120 argued that, with sufficient linker length, the peptide SH could approach and disrupt several alternative gp120 disulfides. Inhibition of lysis by gp120 mAb 2G12, which binds at the base of the V3 loop, as well as disulfide mutational effects, argued that PTT-induced disruption of the gp120 disulfide cluster at the base of the V3 loop is an important step in lytic inactivation of HIV-1. Further, PTT-induced lysis was enhanced after treating virus with reducing agents dithiothreitol and tris (2-carboxyethyl)phosphine. Overall, the results are consistent with the view that the binding of PTT positions the peptide SH group to interfere with conserved disulfides clustered proximal to the CD4 binding site in gp120, leading to disulfide exchange in gp120 and possibly gp41, rearrangement of the Env spike, and ultimately disruption of the viral membrane. The dependence of lysis activity on thiol-disulfide interaction may be related to intrinsic disulfide exchange susceptibility in gp120 that has been reported previously to play a role in HIV-1 cell infection. PMID:26458166

  8. Enfermedades de base genética Genetically based diseases

    Directory of Open Access Journals (Sweden)

    D. González-Lamuño

    2008-01-01

    Full Text Available La genética constituye uno de los mayores avances científicos del siglo XX, que comienza con el redescubrimiento de las leyes de Mendel y termina con la elaboración del primer "borrador" de la secuencia completa del genoma humano. La genética utiliza diferentes estrategias de investigación, como los estudios de gemelos y de adopción, que investigan la influencia de los factores genéticos y ambientales, y las estrategias para identificar genes específicos (genética molecular. Además del importante grado de discapacidad que generan, el impacto social de las enfermedades hereditarias es enorme, por su carácter potencialmente recurrente en una misma familia y por el elevado coste socio-sanitario derivado de la enorme carga de cuidados que requiere. El diagnóstico de las enfermedades hereditarias presenta características diferenciadoras muy significativas ya que el resultado de un diagnóstico genético tiene no sólo efectos sobre el paciente sino también sobre todos los individuos emparentados. Por tanto, la unidad de estudio en el diagnóstico genético es la familia y todo proceso de diagnóstico implica una investigación familiar. También conviene tener en cuenta que los protocolos de diagnóstico se desarrollan de forma paralela a la investigación básica y generalmente están poco estandarizados. Los resultados obtenidos en los estudios genéticos y el tipo de información que se facilita al paciente y a su familia deben ser matizados dentro del proceso del "consejo genético".Genetics is one of the greatest scientific advances of the XX century, which begins with the rediscovery of Mendel’s laws and culminates in the elaboration of the first "draft" of the complete sequence of the human genome. Genetics employs different research strategies, such as the study of twins and adoption, investigating the influence of genetic and environmental factors, and strategies for identifying specific genes (molecular genetics. Besides the

  9. Warum Englisch allein als Wissenschaftssprache nicht genügt

    OpenAIRE

    Baschera, Marco

    2010-01-01

    Einst war Latein Lingua franca der intellektuellen Welt. Gegenwärtig wird darüber debattiert, ob sich Englisch dafür eignet. Doch Latein war eine künstliche Gelehrtensprache, während Englisch heute einem unkontrollierbaren Sprachwandel unterworfen ist. Kulturell unterschiedene Denkweisen vermag es nicht zu spiegeln.

  10. Enhanced cell surface expression, immunogenicity and genetic stability resulting from a spontaneous truncation of HIV Env expressed by a recombinant MVA

    International Nuclear Information System (INIS)

    During propagation of modified vaccinia virus Ankara (MVA) encoding HIV 89.6 Env, a few viral foci stained very prominently. Virus cloned from such foci replicated to higher titers than the parent and displayed enhanced genetic stability on passage. Sequence analysis showed a single nucleotide deletion in the 89.6 env gene of the mutant that caused a frame shift and truncation of 115 amino acids from the cytoplasmic domain. The truncated Env was more highly expressed on the cell surface, induced higher antibody responses than the full-length Env, reacted with HIV neutralizing monoclonal antibodies and mediated CD4/co-receptor-dependent fusion. Intramuscular (IM), intradermal (ID) needleless, and intrarectal (IR) catheter inoculations gave comparable serum IgG responses. However, intraoral (IO) needleless injector route gave the highest IgA in lung washings and IR gave the highest IgA and IgG responses in fecal extracts. Induction of CTL responses in the spleens of individual mice as assayed by intracellular cytokine staining was similar with both the full-length and truncated Env constructs. Induction of acute and memory CTL in the spleens of mice immunized with the truncated Env construct by ID, IO, and IR routes was comparable and higher than by the IM route, but only the IR route induced CTL in the gut-associated lymphoid tissue. Thus, truncation of Env enhanced genetic stability as well as serum and mucosal antibody responses, suggesting the desirability of a similar modification in MVA-based candidate HIV vaccines

  11. A pilot study comparing the development of EIAV Env-specific antibodies induced by DNA/recombinant vaccinia-vectored vaccines and an attenuated Chinese EIAV vaccine.

    Science.gov (United States)

    Meng, Qinglai; Lin, Yuezhi; Ma, Jian; Ma, Yan; Zhao, Liping; Li, Shenwei; Yang, Kai; Zhou, Jianhua; Shen, Rongxian; Zhang, Xiaoyan; Shao, Yiming

    2012-12-01

    Data from successful attenuated lentiviral vaccine studies indicate that fully mature Env-specific antibodies characterized by high titer, high avidity, and the predominant recognition of conformational epitopes are associated with protective efficacy. Although vaccination with a DNA prime/recombinant vaccinia-vectored vaccine boost strategy has been found to be effective in some trials with non-human primate/simian/human immunodeficiency virus (SHIV) models, it remains unclear whether this vaccination strategy could elicit mature equine infectious anemia virus (EIAV) Env-specific antibodies, thus protecting vaccinated horses against EIAV infection. Therefore, in this pilot study we vaccinated horses using a strategy based on DNA prime/recombinant Tiantan vaccinia (rTTV)-vectored vaccines encoding EIAV env and gag genes, and observed the development of Env-specific antibodies, neutralizing antibodies, and p26-specific antibodies. Vaccination with DNA induced low titer, low avidity, and the predominant recognition of linear epitopes by Env-specific antibodies, which was enhanced by boosting vaccinations with rTTV vaccines. However, the maturation levels of Env-specific antibodies induced by the DNA/rTTV vaccines were significantly lower than those induced by the attenuated vaccine EIAV(FDDV). Additionally, DNA/rTTV vaccines did not elicit broadly neutralizing antibodies. After challenge with a virulent EIAV strain, all of the vaccinees and control horses died from EIAV disease. These data indicate that the regimen of DNA prime/rTTV vaccine boost did not induce mature Env-specific antibodies, which might have contributed to immune protection failure. PMID:23171359

  12. Del pasado al futuro de las razas bovinas de carne autóctonas. Análisis genealógico y de marcadores SNP para la implementación de la selección genómica

    OpenAIRE

    Cañas Álvarez, Jhon Jacobo

    2015-01-01

    Las técnicas actuales de genotipado masivo de marcadores SNP han proporcionado una herramienta muy útil, tanto para determinar la diversidad como para la mejora genética animal. Sin embargo, en las razas españolas de vacuno de carne su aplicabilidad no ha sido tan evidente hasta ahora. Es por esta razón, que las preguntas más importantes que se plantearon en esta tesis doctoral fueron: 1) determinar la cantidad de variación genética que hay en las principales razas autóctonas de ganado de car...

  13. Estudi comparatiu de l'estructura del gen "Adh" a vàries espècies de "Drosophila"

    OpenAIRE

    Marfany i Nadal, Gemma

    1991-01-01

    [cat] S'ha caracterizat l'estructura de la regió genòmica del gen "Adh" a quatre espècies del gènere "Drosophila", pertanyents al subgrup "obscura": "D. ambigua", "D. subobscura", "D. madeirensis" i "D. guanche" amb els objectius d'analitzar l'organització i l'evolució d'aquesta regió genòmica dins d'aquest gènere i clarificar les relacions filogenètiques d'aquestes espècies. Per assolir aquestes fites, es van construir llibreries genòmiques de cada espècie que es van crivellar amb una sonda ...

  14. Preserving Accuracy in GenBank

    DEFF Research Database (Denmark)

    Bidartondo, M.I.; Bruns, T. D.; Blackwell, M.;

    2008-01-01

    GenBank, the public repository for nucleotide and protein sequences, is a critical resource for molecular biology, evolutionary biology, and ecology. While some attention has been drawn to sequence errors (1), common annotation errors also reduce the value of this database. In fact, for organisms...... such as fungi, which are notoriously difficult to identify, up to 20% of DNA sequence records may have erroneous lineage designations in GenBank (2). Gene function annotation in protein sequence databases is similarly error-prone (3, 4). Because identity and function of new sequences are often...... unsustainable over the long term as authors eventually leave the field. Although it is possible to link third-party databases to GenBank records, this is a short-term solution that has little guarantee of permanence. Similarly, the current third-party annotation option in GenBank (TPA) complicates rather than...

  15. The conserved His8 of the Moloney murine leukemia virus Env SU subunit directs the activity of the SU-TM disulphide bond isomerase

    International Nuclear Information System (INIS)

    Murine leukemia virus (MLV) fusion is controlled by isomerization of the disulphide bond between the receptor-binding surface (SU) and fusion-active transmembrane subunits of the Env-complex. The bond is in SU linked to a CXXC motif. This carries a free thiol that upon receptor binding can be activated (ionized) to attack the disulphide and rearrange it into a disulphide isomer within the motif. To find out whether His8 in the conserved SPHQ sequence of Env directs thiol activation, we analyzed its ionization in MLV vectors with wtEnv and Env with His8 deleted or substituted for Tyr or Arg, which partially or completely arrests fusion. The ionization was monitored by following the pH effect on isomerization in vitro by Ca2+ depletion or in vivo by receptor binding. We found that wtEnv isomerized optimally at slightly basic pH whereas the partially active mutant required higher and the inactive mutants still higher pH. This suggests that His8 directs the ionization of the CXXC thiol

  16. Intercompartmental recombination of HIV-1 contributes to env intrahost diversity and modulates viral tropism and sensitivity to entry inhibitors.

    Science.gov (United States)

    Brown, Richard J P; Peters, Paul J; Caron, Catherine; Gonzalez-Perez, Maria Paz; Stones, Leanne; Ankghuambom, Chiambah; Pondei, Kemebradikumo; McClure, C Patrick; Alemnji, George; Taylor, Stephen; Sharp, Paul M; Clapham, Paul R; Ball, Jonathan K

    2011-06-01

    HIV-1 circulates within an infected host as a genetically heterogeneous viral population. Viral intrahost diversity is shaped by substitutional evolution and recombination. Although many studies have speculated that recombination could have a significant impact on viral phenotype, this has never been definitively demonstrated. We report here phylogenetic and subsequent phenotypic analyses of envelope genes obtained from HIV-1 populations present in different anatomical compartments. Assessment of env compartmentalization from immunologically discrete tissues was assessed utilizing a single genome amplification approach, minimizing in vitro-generated artifacts. Genetic compartmentalization of variants was frequently observed. In addition, multiple incidences of intercompartment recombination, presumably facilitated by low-level migration of virus or infected cells between different anatomic sites and coinfection of susceptible cells by genetically divergent strains, were identified. These analyses demonstrate that intercompartment recombination is a fundamental evolutionary mechanism that helps to shape HIV-1 env intrahost diversity in natural infection. Analysis of the phenotypic consequences of these recombination events showed that genetic compartmentalization often correlates with phenotypic compartmentalization and that intercompartment recombination results in phenotype modulation. This represents definitive proof that recombination can generate novel combinations of phenotypic traits which differ subtly from those of parental strains, an important phenomenon that may have an impact on antiviral therapy and contribute to HIV-1 persistence in vivo. PMID:21471230

  17. Unleashing Gen Y: Marketing Mars to Millennials

    Science.gov (United States)

    Leahy, Bart D.; Hidalgo, Loretta; Kloberdanz, Cassie

    2007-01-01

    Space advocates need to engage Generation Y (born 1977-1999).This outreach is necessary to recruit the next generation of scientists and engineers to explore Mars. Space advocates in the non-profit, private, and government sectors need to use a combination of technical communication, marketing, and politics, to develop messages that resonate with Gen Y. Until now, space messages have been generated by and for college-educated white males; Gen Y is much more diverse, including as much as one third minorities. Young women, too, need to be reached. My research has shown that messages emphasizing technology, fun, humor, and opportunity are the best means of reaching the Gen Y audience of 60 million (US population is 300 million). The important things space advocates must avoid are talking down to this generation, making false promises, or expecting them to "wait their turn" before they can participate. This is the MTV generation! We need to find ways of engaging Gen Y now to build a future where human beings can live and work on the planet Mars. In addition to the messages themselves, advocates need to keep up with Gen Y' s social networking and use of iPods, cell phones, and the Internet. NASA and space advocacy groups can use these tools for "viral marketing," where young people share targeted space-related information via cell phones or the Internet because they like it. Overall, Gen Y is a socially dynamic and media-savvy group; advocates' space messages need to be sincere, creative, and placed in locations where Gen Y lives. Mars messages must be memorable!

  18. Dense display of HIV-1 envelope spikes on the lambda phage scaffold does not result in the generation of improved antibody responses to HIV-1 Env

    OpenAIRE

    Mattiacio, Jonelle; Walter, Scott; Brewer, Matt; Domm, William; Friedman, Alan E.; Dewhurst, Stephen

    2011-01-01

    The generation of strong, virus-neutralizing antibody responses to the HIV-1 envelope spike (Env) is a major goal in HIV-1 vaccine research. To try to enhance the Env-specific response, we displayed oligomeric gp140 on a virus-like scaffold provided by the lambda phage capsid. To do this, an in vitro complementation system was used to “decorate” phage particles with glycosylated, mammalian cell-derived envelope oligomers. We compared the immune response to lambda phage particles displaying HI...

  19. Immunological responses during a virologically failing antiretroviral regimen are associated with in vivo synonymous mutation rates of HIV type-1 env

    DEFF Research Database (Denmark)

    Mens, Helene; Jørgensen, Louise Bruun; Kronborg, Gitte;

    2009-01-01

    BACKGROUND: Little is known about the underlying causes of differences in immunological response to antiretroviral therapy during multidrug-resistant (MDR) HIV type-1 (HIV-1) infection. This study aimed to identify virological factors associated with immunological response during therapy failure....... METHODS: Individuals with MDR HIV-1 receiving therapy for > or =3 months were included. CD4+ T-cell count slopes and pol and clonal env sequences were determined. Genetic analyses were performed using distance-based and maximum likelihood methods. Synonymous mutations rates of env were used to estimate...

  20. Implication of the env Gene of the Human Endogenous Retrovirus W Family in the Expression of BDNF and DRD3 and Development of Recent-Onset Schizophrenia

    OpenAIRE

    Huang, Wenjie; Li, Shan; Hu, Yuanming; Yu, Honglian; Luo, Feng; Zhang, Qi; Zhu, Fan

    2010-01-01

    Objective: Retrovirus has been suggested as one of agents involved in the development of schizophrenia. In the present study, we examined the role of the human endogenous retrovirus W family (HERV-W) env gene in the etiopathogenesis of recent-onset schizophrenia, using molecular and epidemiological approaches. Methods: Nested RT-PCR was used to detect the messenger RNA (mRNA) of the HERV-w env gene in plasmas. Quantitative real-time polymerase chain reaction (PCR) was employed to detect the v...

  1. A Study of Low pH-Induced Refolding of Env of Avian Sarcoma and Leukosis Virus into a Six-Helix Bundle

    OpenAIRE

    Markosyan, R. M.; P. Bates; Cohen, F S; Melikyan, G B

    2004-01-01

    The fusion protein of avian sarcoma and leukosis virus is likely to fold into a six-helix bundle as part of its final configuration. A peptide, R99, inhibits fusion, probably by binding into the grooves of the triple-stranded coiled coil that becomes the central core of the six-helix bundle. The stages at which the envelope protein (Env) of avian sarcoma and leukosis virus subgroup A folds into a bundle during low pH-induced fusion were determined. Effector cells expressing Env were bound to ...

  2. Aislamiento y caracterización de ABG1, un gen esencial del hongo patógeno oportunista Candida Albicans.

    OpenAIRE

    Veses Jiménez, Verónica

    2005-01-01

    RESUMEN Mediante el inmunorrastreo de una genoteca de expresión de Candida albicans con un anticuerpo policlonal específico de la forma micelial de dicho hongo (PAb anti-gt), se ha aislado un nuevo gen de este microorganismo. La represión del gen aislado ocasionó un severo defecto en la morfogénesis de C. albicans, caracterizado por la formación de cadenas de levaduras, de tamaño decreciente en dirección al extremo apical de la cadena, por lo que el gen fue denominado ABG1 (del inglés, alt...

  3. VARIABILIDAD DEL GEN NUCLEAR G3PDH EN JATROPHA CURCAS L.

    Directory of Open Access Journals (Sweden)

    Sonia Castro Guzmán

    2015-11-01

    Full Text Available Jatrophacurcas L.es una especie nativa de América tropical; en nuestro país se ha venido utilizando principalmente como medicinal y alimenticia desde la época prehispánica. Actualmente el aceite extraído de sus semillas ha adquirido importancia internacional ya que puede ser transformado a biodiesel. El conocimiento que existe sobre la variabilidad genética de la especie es escaso. Un gen utilizado con éxito para el estudio de patrones de variación y origen de la yuca (ManihotesculentaL. y del caco (Theobromacacao L. es el gen nuclearG3pdh(Gliceraldehído3 fosfato deshidrogenasa involucrado en la fotosíntesis. Con base en ello, en este trabajo se exploró la variabilidad del gen G3pdh en individuos deJ. curcasprovenientes de 15 poblaciones de los estados de Veracruz, Campeche, Yucatán y Quintana Roo. Para obtener el gen G3pdh completo de alrededor de 1000 pares de bases, se amplificó utilizando losprimersdiseñados porStrandet al. (1997 y los dosprimersinternos reverse diseñados porOlsenySchaal(1999 para obtener segmentos más cortos, de 600 y 800 pares de bases aproximadamente. Por primera vez se amplificaron aproximadamente 500pby los resultados demuestran que el gen G3pdh es útil para analizar la variabilidad deJ. curcas, y con un importante potencial para evaluar la distribución y evolución de sus poblaciones en México, conocer las relaciones ancestro descendiente a nivel poblacional y explicar las causas de la distribución de los distintoshaplotipos.

  4. Mapas genéticos em plantas

    OpenAIRE

    Carneiro Monalisa Sampaio; Vieira Maria Lucia Carneiro

    2002-01-01

    Ao lado dos projetos de seqüenciamento e das análises do cariótipo pelas técnicas de hibridização in situ, o desenvolvimento de mapas genéticos fundamentados em marcadores de DNA tem propiciado consideráveis avanços à genômica de plantas. Esta revisão aborda as premissas básicas utilizadas para o mapeamento genético e suas principais aplicações, especialmente para o melhoramento vegetal. Fundamentos teóricos sobre segregação, recombinação e ligação são considerados e relacionados à construção...

  5. Genómica funcional de la elongación transcripcional

    OpenAIRE

    Rodríguez Gil, Alfonso

    2008-01-01

    Los principales objetivos de esta Tesis Doctoral son: Desarrollo de un nuevo método para el estudio de la distribución intragénica de la RNA polimerasa II a escala genómica. Aplicación del método desarrollado al estudio de mutantes afectados en la elongación transcripcional. Identificación de nuevos factores que afectan a la elongación transcripcional

  6. Kontrolle der Expression des UNUSUAL FLORAL ORGANS (UFO) Gens in Arabidopsis thaliana

    OpenAIRE

    Hobe, Martin

    2004-01-01

    Die vorliegende Arbeit befaßt sich mit der Kontrolle des Expressionsmusters des UNUSUAL FLORAL ORGANS (UFO) Gens von Arabidopsis thaliana. UFO wird im Sproß- und Blütenmeristemen aller Entwicklungsstadien der Pflanze exprimiert. In Blütenmeristemen agiert UFO als Kofaktor von LEAFY (LFY) bei der Aktivierung der Organidentitätsgene des zweiten und dritten Wirtels. UFO stellt also einen generellen Faktor der Musterbildung in Meristemen dar. Um regulatorische Gene, die die Expression von UFO bee...

  7. GenBank blastx search result: AK062648 [KOME

    Lifescience Database Archive (English)

    Full Text Available smembrane helix receptor pseudogene, the 3' end of NR5A1 gen...e for nuclear receptor (subfamily 5, group A) member 1, a novel gene and four CpG islands, complete sequence.|PRI PRI 4e-74 +2 ... ...EK6 gene for never in mitosis gene a (NIMA)-related kinase 6, a pseudogene similar to part of neuronal pentraxin II, a seven tran...AK062648 001-105-E04 AL137846.24 Human DNA sequence from clone RP11-101K10 on chrom...osome 9 Contains the PSMB7 gene for proteasome (prosome, macropain) beta type 7 subunit, the 3' end of the N

  8. GenBank blastx search result: AK112000 [KOME

    Lifescience Database Archive (English)

    Full Text Available smembrane helix receptor pseudogene, the 3' end of NR5A1 gen...e for nuclear receptor (subfamily 5, group A) member 1, a novel gene and four CpG islands, complete sequence.|PRI PRI 2e-12 +3 ... ...EK6 gene for never in mitosis gene a (NIMA)-related kinase 6, a pseudogene similar to part of neuronal pentraxin II, a seven tran...AK112000 001-035-B07 AL137846.24 Human DNA sequence from clone RP11-101K10 on chrom...osome 9 Contains the PSMB7 gene for proteasome (prosome, macropain) beta type 7 subunit, the 3' end of the N

  9. GenBank blastx search result: AK058440 [KOME

    Lifescience Database Archive (English)

    Full Text Available smembrane helix receptor pseudogene, the 3' end of NR5A1 gen...e for nuclear receptor (subfamily 5, group A) member 1, a novel gene and four CpG islands, complete sequence.|PRI PRI 2e-59 +1 ... ...EK6 gene for never in mitosis gene a (NIMA)-related kinase 6, a pseudogene similar to part of neuronal pentraxin II, a seven tran...AK058440 001-015-F11 AL137846.24 Human DNA sequence from clone RP11-101K10 on chrom...osome 9 Contains the PSMB7 gene for proteasome (prosome, macropain) beta type 7 subunit, the 3' end of the N

  10. GenBank blastx search result: AK104361 [KOME

    Lifescience Database Archive (English)

    Full Text Available smembrane helix receptor pseudogene, the 3' end of NR5A1 gen...e for nuclear receptor (subfamily 5, group A) member 1, a novel gene and four CpG islands, complete sequence.|PRI PRI 2e-15 +3 ... ...EK6 gene for never in mitosis gene a (NIMA)-related kinase 6, a pseudogene similar to part of neuronal pentraxin II, a seven tran...AK104361 001-035-C04 AL137846.24 Human DNA sequence from clone RP11-101K10 on chrom...osome 9 Contains the PSMB7 gene for proteasome (prosome, macropain) beta type 7 subunit, the 3' end of the N

  11. Regulation of Gag- and Env-Specific CD8+ T Cell Responses in ART-Naive HIV-Infected Patients: Potential Implications for Individualized Immunotherapy.

    Directory of Open Access Journals (Sweden)

    Christian Prebensen

    Full Text Available Strategies to develop a functional cure for HIV infection will likely require boosting of effector T cell responses to eliminate reactivated, latently infected cells. We have recently explored an assay for assessing antigen-specific regulation of T cell proliferation, which was related to clinical progression in untreated patients and to vaccine efficacy in two trials of therapeutic Gag-based vaccines. We here expand the same assay to further investigate regulation mediated by various inhibitory pathways. Peripheral blood mononuclear cells from 26 asymptomatic HIV-infected, antiretroviral therapy-naïve patients were stimulated with Gag and Env overlapping peptide panels for 5 days. Monoclonal antibodies (mAbs blocking inhibitory mediators interleukin (IL 10, transforming growth factor (TGF β, programmed death ligand (PD-L 1 and herpes virus entry mediator (HVEM were added to parallel cultures. Functional T cell regulation (FTR was defined as the difference in proliferation between stimulated cultures with and without blocking mAbs. FTR was detected in 54% of patients. Blockade of IL-10/PD-L1 and IL10/TGF-β detected all cases with Gag- and Env-associated FTR, respectively. In accordance with previous findings, isolated Env FTR was associated with higher plasma HIV RNA and lower CD4 counts, while patients with both Gag and Env FTR also had higher Gag- and Env-specific proliferative CD8+ T cell responses. There was no association between FTR and frequencies of activated regulatory T cells. In conclusion, we observed substantial heterogeneity in FTR between patients, inhibitory pathways and HIV antigens. FTR may help to individualize immunomodulation and warrants further assessment in clinical immunotherapy trials.

  12. Regulation of Gag- and Env-Specific CD8+ T Cell Responses in ART-Naïve HIV-Infected Patients: Potential Implications for Individualized Immunotherapy

    Science.gov (United States)

    Prebensen, Christian; Lind, Andreas; Dyrhol-Riise, Anne-Ma; Kvale, Dag

    2016-01-01

    Strategies to develop a functional cure for HIV infection will likely require boosting of effector T cell responses to eliminate reactivated, latently infected cells. We have recently explored an assay for assessing antigen-specific regulation of T cell proliferation, which was related to clinical progression in untreated patients and to vaccine efficacy in two trials of therapeutic Gag-based vaccines. We here expand the same assay to further investigate regulation mediated by various inhibitory pathways. Peripheral blood mononuclear cells from 26 asymptomatic HIV-infected, antiretroviral therapy-naïve patients were stimulated with Gag and Env overlapping peptide panels for 5 days. Monoclonal antibodies (mAbs) blocking inhibitory mediators interleukin (IL) 10, transforming growth factor (TGF) β, programmed death ligand (PD–L) 1 and herpes virus entry mediator (HVEM) were added to parallel cultures. Functional T cell regulation (FTR) was defined as the difference in proliferation between stimulated cultures with and without blocking mAbs. FTR was detected in 54% of patients. Blockade of IL-10/PD-L1 and IL10/TGF-β detected all cases with Gag- and Env-associated FTR, respectively. In accordance with previous findings, isolated Env FTR was associated with higher plasma HIV RNA and lower CD4 counts, while patients with both Gag and Env FTR also had higher Gag- and Env-specific proliferative CD8+ T cell responses. There was no association between FTR and frequencies of activated regulatory T cells. In conclusion, we observed substantial heterogeneity in FTR between patients, inhibitory pathways and HIV antigens. FTR may help to individualize immunomodulation and warrants further assessment in clinical immunotherapy trials. PMID:27128502

  13. Asociación del gen bola-drb3.2 con el virus de la leucosis bovina (vlb) en ganado criollo hartón del valle

    OpenAIRE

    Posso Terranova, Andrés Mauricio; Muñoz Florez, Jaime Eduardo; Giovambattista, Guillermo; Alvarez Franco, Luz Angela

    2014-01-01

    En cien muestras de ganado criollo hartón del Valle (HV) del Banco de ADN del Laboratorio de genética animal de la Universidad Nacional de Colombia sede Palmira se determinó la presencia del VLB siguiendo la metodología PCR anidado descrita por Beier et al. (2001) y se genotipificaron los animales para el gen DRB3.2* utilizando la metodología de PCR-SBT (Sequence Based Typings) descrita por Takeshima et al. (2009). Se encontró el porcentaje de presencia del virus. Para el gen BoLA-DRB3.2* se ...

  14. Análisis de la variabiblidad genética de Quercus Suber L. mediante marcadores moleculares y su aplicación a la conservación de recursos genéticos

    OpenAIRE

    Jiménez Sancho, María del Pilar

    2000-01-01

    En la presente tesis se realiza el estudio de la variabilidad genética de Quercus suber L. A través de dos tipos de marcadores moleculares: isoenzimas y ADN de cloroplastos. La variación enzimática se estima a partir de 22 poblaciones, mediante 9 loci polimórficos. A partir de las frecuencias alélicas se obtienen diversos parámetros de diversidad genética entre y dentro de poblaciones, que ofrecen valores tipicos de las especies del genero. Se observa una gran diversificación intrapoblacional...

  15. Evolución genómica por diseño molecular de levaduras industriales

    OpenAIRE

    SANI, DANIELE

    2013-01-01

    En esta Tesis Doctoral se propone una alternativa a la coyuntura actual de rechazo social frente al uso de OMGs en la industria agroalimentaria, mediante la demostración y el desarrollo de un nuevo concepto sobre el uso de las técnicas de biología molecular en la obtención de levaduras modificadas genéticamente, el concepto de Evolución Genómica mediante Diseño Molecular. La idea básica de este nuevo concepto es simple y se basa en imitar a la propia naturaleza en su const...

  16. Anomalías y displasias dentarias de origen genético-hereditario Inherited dental abnormalities and dysplasias

    OpenAIRE

    J. Martín-González; B. Sánchez-Domínguez; M.L. Tarilonte-Delgado; L. Castellanos-Cosano; J.M. Llamas-Carreras; F.J. López-Frías; J.J. Segura-Egea

    2012-01-01

    Las alteraciones del desarrollo embriológico de la dentición provocan anomalías y displasias dentarias. Los factores etiopatogénicos implicados en las alteraciones del desarrollo dentario son básicamente dos: genéticos y ambientales. Según la fase del desarrollo en que afecten al órgano del esmalte y a los tejidos dentarios, aparecerán diferentes anomalías y/o displasias dentales. El control genético del desarrollo dentario se lleva a cabo mediante dos procesos: a) control de la histogénesis ...

  17. Los algoritmos genéticos y el método de generación y prueba

    Directory of Open Access Journals (Sweden)

    Luis Roberto Ojeda Ch.

    2011-01-01

    Full Text Available Este trabajo pretende mostrar dos paradigmas de solución de problemas en inteligencia artificial. Los algoritmos genéticos han tomado una posición destacada en los últimos tiempos y el método de generación y prueba, cuyo perfil se aproxima al fundamento de los algoritmos genéticos constituye la forma de diseño de DENDRAL, un producto ampliamente reconocido en IA. Se desea mostrar que ambos paradigmas se ubican naturalmente dentro de las expectativas de la inteligencia artificial.

  18. Los algoritmos genéticos y el método de generación y prueba

    OpenAIRE

    Luis Roberto Ojeda Ch.

    2011-01-01

    Este trabajo pretende mostrar dos paradigmas de solución de problemas en inteligencia artificial. Los algoritmos genéticos han tomado una posición destacada en los últimos tiempos y el método de generación y prueba, cuyo perfil se aproxima al fundamento de los algoritmos genéticos constituye la forma de diseño de DENDRAL, un producto ampliamente reconocido en IA. Se desea mostrar que ambos paradigmas se ubican naturalmente dentro de las expectativas de la inteligencia artificial.

  19. Frecuencias alélicas y fenotípicas de los marcadores genéticos enzimáticos: Fosfoglucomutasa 1 (FGM 1), Esterasa D (EsD) y Fosfatasa ácida eritrocitaria (FAE) en población residente en la ciudad de Medellín, 1996-1997

    OpenAIRE

    María Victoria Hurtado Ángel; Nora María Giraldo Ramírez; Silvia García Jaramillo; Ofelia Calle Avendaño

    2000-01-01

    Este estudio descriptivo de las enzimas fosfoglucomutasa 1 (fgm 1), esterasa D (EsD) y Fosfatasa ácida eritrocitaria (FAE) como marcadores genéticos polimórficos, se realizó en 145 muestras de sangre, tomadas a igual númerode donantes de los tres principales bancos de sangre de la ciudad, por el método de electroforesis convencional, con sistema de refrigeración, en gel de agarosa. La probabilidad de identificación para cada uno de los marcadores fue buena; la mejor fue la subtipificación de ...

  20. Genética de poplaciones de Atta sexdens rubropilosa (Hymenoptera: Formicidae) POPULATION GENETICS OF Atta sexdens rubropilosa (HYMENOPTERA: FORMICIDAE)

    OpenAIRE

    LIRIANA BELIZÁRIO CANTAGALLI; CLAUDETE APARECIDA MANGOLIN; MARIA CLAUDIA COLLA RUVOLO TAKASUSUKI

    2013-01-01

    La variabilidad genética de las hormigas Atta sexdens rubropilosa colectadas en cinco lugares distintos de Brasil fueron evaluados por la técnica PCR-RAPD. Un total de 15 primers produjeron 148 fragmentos, de los cuales 123 fueron polimórficos, lo que corresponden al 83,11 %. La estimación de la diversidad genética por el índice de Shannon fue 0,3836 y el desviación estándar fue de ± 0,2335. Estos valores demuestran una alta diversidad genética. El valor de G ST fue 0,2372 y ΦPT = 0,184 l...

  1. Diagnostico genético prenatal y aborto. Dos cuestiones de eugenesia y discriminación

    OpenAIRE

    FABIOLA VILLELA CORTÉS; Jorge E. Linares Salgado

    2012-01-01

    Los avances en genética seguidos de las nuevas tecnologías en la detección temprana de afecciones genéticas conllevan dilemas bioéticos sobre el uso adecuado de estas técnicas, la información que se le da a la mujer embarazada y la decisión que ella tomará al recibirla. Detectar a tiempo anomalías genéticas permite, en algunas ocasiones, el inicio de un tratamiento adecuado que permita que el niño por nacer no desarrolle una enfermedad discapacitante, como el caso de la fenilcetonuria, o una ...

  2. Editorial: Cartas al Editor

    Directory of Open Access Journals (Sweden)

    Guillermo Llanos

    2006-06-01

    Full Text Available En todas las revistas biomédicas del mundo existe una sección habitual denominada como el título de este editorial y que en Colombia Médica la llamamos correspondencia. Este espacio tiene como objetivo fundamental que los lectores escriban a los autores (investigadores comentando los artículos que se han publicado en números anteriores de la revista. Las cartas son, entonces, breves comentarios de desacuerdo o acuerdo (conceptual, metodológico, interpretativo sobre falencias ubicadas por un lector alerta al tema debatido en cualquiera de las secciones como en los artículos originales, editoriales, revisiones, presentación de casos, etc. publicados previamente en la revista. Las cartas deben permitir el intercambio de opiniones y formas de pensar y analizar los problemas planteados entre los autores y los lectores. En ocasiones una serie de cartas motivadas por un artículo original puede ser tan o más interesante como el artículo que generó la correspondencia. Las cartas son también un formato adecuado para comunicar un hecho novedoso de una observación clínica juiciosa no descrita antes. Un ejemplo de ello es la carta de un gineco-obstetra australiano cuando informó a Lancet los primeros efectos adversos informados de la talidomida. Colombia Médica, a pesar de mantener vigente esta sección, no ha sido muy afortunada en establecer una buena cultura del envío de cartas al editor. Pero últimamente han llegado juiciosas observaciones a las cuales se les ha dado prioridad en su publicación. Toda carta que comenta los hallazgos de un artículo es enviada a los autores para su respuesta, porque ello genera el debate académico buscado, y luego se publican, tanto la carta como su respuesta. El Estilo Vancouver recomienda que la carta y su respuesta deban ser breve, no pasar de dos o tres párrafos y contener sólo cuatro o cinco referencias. En lo posible (a menos que sea indispensable no presentar ni cuadros ni gráficos. El t

  3. Structures and stability of metal-doped GenM (n = 9, 10 clusters

    Directory of Open Access Journals (Sweden)

    Wei Qin

    2015-06-01

    Full Text Available The lowest-energy structures of neutral and cationic GenM (n = 9, 10; M = Si, Li, Mg, Al, Fe, Mn, Pb, Au, Ag, Yb, Pm and Dy clusters were studied by genetic algorithm (GA and first-principles calculations. The calculation results show that doping of the metal atoms and Si into Ge9 and Ge10 clusters is energetically favorable. Most of the metal-doped Ge cluster structures can be viewed as adding or substituting metal atom on the surface of the corresponding ground-state Gen clusters. However, the neutral and cationic FeGe9,10,MnGe9,10 and Ge10Al are cage-like with the metal atom encapsulated inside. Such cage-like transition metal doped Gen clusters are shown to have higher adsorption energy and thermal stability. Our calculation results suggest that Ge9,10Fe and Ge9Si would be used as building blocks in cluster-assembled nanomaterials because of their high stabilities.

  4. Avances genéticos y moleculares en el estudio de trastornos mentales

    Directory of Open Access Journals (Sweden)

    Daisy Natalia Salamanca-Ortíz

    2014-04-01

    Full Text Available Actualmente, con el aumento de la disponibilidad de técnicas para la realización de estudios genéticos, han aparecido nuevas áreas del conocimiento como la epigenética y la farmacogenética. Estas nuevas áreas han permitido esclarecer las bases genéticas implicadas en la aparición de las enfermedades que afectan al ser humano. Dada su aplicación global, la psiquiatría no ha sido ajena al alcance de estas nuevas disciplinas, lo cual se refleja en el gran número de estudios realizados a nivel mundial que han relacionado la presencia de variantes genéticas en los individuos con la aparición de trastornos mentales específicos. De la misma forma, otros estudios han evidenciado que la efectividad de los tratamientos farmacológicos empleados en psiquiatría se correlaciona con polimorfismos en las enzimas encargadas de metabolizar dichos medicamentos. Por lo tanto, es importante que los psiquiatras conozcan los aspectos básicos de estas relaciones para obtener mejores resultados en el diagnóstico y tratamiento de los principales trastornos mentales.

  5. Structures and stability of metal-doped GenM (n = 9, 10) clusters

    International Nuclear Information System (INIS)

    The lowest-energy structures of neutral and cationic GenM (n = 9, 10; M = Si, Li, Mg, Al, Fe, Mn, Pb, Au, Ag, Yb, Pm and Dy) clusters were studied by genetic algorithm (GA) and first-principles calculations. The calculation results show that doping of the metal atoms and Si into Ge9 and Ge10 clusters is energetically favorable. Most of the metal-doped Ge cluster structures can be viewed as adding or substituting metal atom on the surface of the corresponding ground-state Gen clusters. However, the neutral and cationic FeGe9,10,MnGe9,10 and Ge10Al are cage-like with the metal atom encapsulated inside. Such cage-like transition metal doped Gen clusters are shown to have higher adsorption energy and thermal stability. Our calculation results suggest that Ge9,10Fe and Ge9Si would be used as building blocks in cluster-assembled nanomaterials because of their high stabilities

  6. Safety Assurance in NextGen

    Science.gov (United States)

    HarrisonFleming, Cody; Spencer, Melissa; Leveson, Nancy; Wilkinson, Chris

    2012-01-01

    The generation of minimum operational, safety, performance, and interoperability requirements is an important aspect of safely integrating new NextGen components into the Communication Navigation Surveillance and Air Traffic Management (CNS/ATM) system. These requirements are used as part of the implementation and approval processes. In addition, they provide guidance to determine the levels of design assurance and performance that are needed for each element of the new NextGen procedures, including aircraft, operator, and Air Navigation and Service Provider. Using the enhanced Airborne Traffic Situational Awareness for InTrail Procedure (ATSA-ITP) as an example, this report describes some limitations of the current process used for generating safety requirements and levels of required design assurance. An alternative process is described, as well as the argument for why the alternative can generate more comprehensive requirements and greater safety assurance than the current approach.

  7. Genève grandeur cultures.

    Directory of Open Access Journals (Sweden)

    Vincent Kaufmann

    2005-04-01

    Full Text Available Equinoxe , revue genevoise de sciences humaines, consacre son dernier numéro à l’émergence et au développement des contre-cultures à Genève depuis la fin des années 1960. Celui-ci se présente comme « une réflexion critique sur trois décennies de pratiques culturelles et sur la manière dont celles-ci sont parvenues non sans heurts à se faire une place dans la cité ». L’intérêt de la démarche réside dans le fait qu’à Genève, contrairement à Berlin, Paris, Barcelone ...

  8. Gen. Keen discusses Americas with JFSSPP

    OpenAIRE

    2011-01-01

    Army Lt. Gen. P. K. Keen, he Militay Deputy Commander for USSOUTHCOM, met with Foreign Area Officers (FAOs) attending a Latin American in-residence course at NPS for the School of International Graduate Studies (SIGS) Joint Foreign Area OFficer Skill Sustainment Pilot Program (JFSSPP). Keen spoke for nearly an hour to more than 35 participants in the course, which ran from February 21 though March 4. JFSSPP students are non-matriculated students who come to NPS for topical short courses rela...

  9. Parámetros y tendencias genéticas para características de crecimiento predestete en la población mexicana de Simmental

    OpenAIRE

    Javier Rosales; Elzo, Mauricio A.; Moisés Montaño; Vicente Eliezer Vega

    2004-01-01

    Se utilizaron registros de pesos al nacimiento (18,383) y al destete (9,023) de la población mexicana de ganado Simmental, para estimar varianzas genéticas aditivas directas y maternas, heredabilidades, correlaciones y tendencias genéticas. La información se obtuvo en 434 hatos de socios de la Asociación Mexicana Simmental-Simbrah, entre 1980 y 1999. El grupo contemporáneo se definió como hatoaño- estación. Se utilizó un modelo animal de dos características con efectos aditivos directos y mat...

  10. Mucopolisacaridosis II: nueva mutación patogénica en gen IDS

    Directory of Open Access Journals (Sweden)

    Eugenia Pérez-Elizondo

    2014-12-01

    Full Text Available La mucopolisacaridosis tipo II es una enfermedad lisosomal producida por la deficiencia de la enzima iduronato 2 sulfatasa. Es una condición infrecuente de herencia recesiva ligada al X, que puede producir importante discapacidad progresiva. El análisis molecular es una técnica útil en la confirmación diagnóstica, que además permite detección de portadores asintomáticos, brindando la oportunidad de asesoría genética. Se presenta el caso de un paciente con mucopolisacaridosis tipo II, en quien se documentó una nueva mutación patogénica en el Gen IDS.

  11. SFRs and GEN IV: ASN actions

    International Nuclear Information System (INIS)

    ASN carries out several actions regarding the 4th generation of reactors (GEN IV) and the sodium fast reactors (SFRs). First of all, ASN considers that GEN IV reactors have to be safer than the EPR reactor currently under construction in France (at Flamanville). Regarding GEN IV in general, the “generation IV international Forum” (GIF) has identified six technologies of reactors for which the possibility of an industrial development could be considered (SFR, GFR, HTR/VHTR, LFR, MSR, SCWR) ; these six technologies include fast reactors. Based on documents sent by French nuclear operators at its request, ASN will organize a technical expertise, with the technical safety organization IRSN and ASN Advisory Committees, in order to have an overview, in terms of safety, R&D needs and possibility of transmutation of these six technologies. The conclusions of the technical expertise are expected by the end of 2013. Concerning SFR in particular, the French operator CEA submitted to ASN in June 2012 a file presenting the general safety orientations of the prototype reactor ASTRID (Advanced Sodium Technological Reactor for Industrial Demonstration). ASN has organized, with IRSN and ASN Advisory Committees, a technical expertise to analyse this file. The conclusions of this technical expertise are expected by mid-2013. Besides, a specific analysis is going on about the transmutation. The conclusions of the analysis could be known in 2013, subject to the transmission of documents by the end of 2012

  12. Immunological responses during a virologically failing antiretroviral regimen are associated with in vivo synonymous mutation rates of HIV type-1 env

    DEFF Research Database (Denmark)

    Mens, Helene; Jørgensen, Louise Bruun; Kronborg, Gitte;

    2009-01-01

    BACKGROUND: Little is known about the underlying causes of differences in immunological response to antiretroviral therapy during multidrug-resistant (MDR) HIV type-1 (HIV-1) infection. This study aimed to identify virological factors associated with immunological response during therapy failure....... METHODS: Individuals with MDR HIV-1 receiving therapy for > or =3 months were included. CD4+ T-cell count slopes and pol and clonal env sequences were determined. Genetic analyses were performed using distance-based and maximum likelihood methods. Synonymous mutations rates of env were used to estimate...... viral replication. RESULTS: Of 1,000 patients treated between 1995 and 2003, 72 individuals fulfilled the definition for triple-class failure, but 25 were non-compliant, 21 were successfully resuppressed and 3 had died or quit therapy. Of the 23 that fulfilled study criteria, 16 had samples available...

  13. Aïllament, identificació i caracterització de nematodes entomopatògens en sòls de cultius del Baix Llobregat i les muntanyes de Prades i possible utilització per al control de plagues

    OpenAIRE

    Suárez Cano, Pedro

    2015-01-01

    Durant els últims anys s'estan realitzant nombrosos estudis sobre l'ús de nematodes com a mètode de control biològic. Mitjançant el present treball s'ha pretès realitzar un aïllament de nematodes entomopatògens en les zones de Prades i del Baix Llobregat i un cop aïllats i identificats realitzar diferents proves d'eficàcia d'aquests aïllats com mètodes de control de diferents plagues. Per dur a terme aquests assajos s'han realitzat diferents sistemes pel manteniment i producció de les pob...

  14. Generic classification of the Archiborborinae (Diptera: Sphaeroceridae), with a revision of Antrops Enderlein, Coloantrops gen. nov., Maculantrops gen. nov., Photoantrops gen. nov., and Poecilantrops gen. nov.

    Science.gov (United States)

    Kits, Joel H; Marshall, Stephen A

    2013-01-01

    The Archiborborinae comprise a diverse clade of flies in the family Sphaeroceridae. We here revise the generic classification, redefining the genus Antrops Enderlein and naming 5 new genera: Boreantrops gen. nov., Coloantrops gen. nov., Maculantrops gen. nov., Photoantrops gen. nov., and Poecilantrops gen. nov. The genus Archiborborus, until recently a paraphyletic assemblage including most of the described species in the subfamily, is treated as a junior synonym of Antrops (syn. nov.) We revise the genera Antrops (53 species, including 40 sp. nov.: Antrops anovariegatus, Antrops aurantifemur, Antrops baeza, Antrops bellavista, Antrops biflavus, Antrops bucki, Antrops carpishensis, Antrops cochabamba, Antrops cochinoca, Antrops coniobaptos, Antrops coroico, Antrops cotopaxi, Antrops didactylos, Antrops diversipennis, Antrops eurus, Antrops fulgiceps, Antrops fuliginosus, Antrops guandera, Antrops guaramacalensis, Antrops inca, Antrops juninensis, Antrops mucarensis, Antrops niger, Antrops papallacta, Antrops pecki, Antrops podocarpus, Antrops quadrilobus, Antrops siberia, Antrops sierrazulensis, Antrops tachira, Antrops tequendama, Antrops tetrastichus, Antrops tumbrensis, Antrops unduavi, Antrops variegatus, Antrops versabilis, Antrops vittatus, Antrops yungas, and Antrops zongo and the following comb. nov.: Antrops annulatus (Richards), Antrops chaetosus (Richards), Antrops femoralis (Blanchard), Antrops hirtus (Bigot), Antrops maculipennis (Duda), Antrops maximus (Richards), Antrops microphthalmus (Richards), Antrops quadrinotus (Bigot), Antrops setosus (Duda), Antrops simplicimanus (Richards), Antrops nitidicollis (Becker), and Antrops orbitalis (Duda)), Coloantrops (1 species: Coloantrops daedalus, sp. nov.), Maculantrops (2 species, Maculantrops hirtipes (Macquart) comb. nov. and Maculantrops altiplanus, sp. nov.), Photoantrops (1 species: Pho-toantrops echinus sp. nov.), and Poecilantrops (10 species: Poecilantrops baorucensis, Poecilantrops boraceiensis

  15. Market share scenarios for Gen-DIII and gen-IV reactors in Europe

    International Nuclear Information System (INIS)

    Nuclear energy is back on the agenda worldwide in order to meet growing energy demand and especially the growth in electricity demand. Many objectives direct to an increased use of nuclear energy, i.e. minimising energy costs, reducing climate change effects and others. In the light of the potential renewed growth of nuclear energy, the public demands a clear view on what nuclear energy may contribute towards meeting these objectives and especially how nuclear energy may address some socio-political obstructions with respect to economics, radioactive waste, safety and proliferation of fissile materials. To address these questions, the future nuclear reactor park mix in Europe has been analysed applying an integrated dynamic process modelling technique. Various market share scenarios for nuclear energy are derived including sub-variants with regard to the intra-nuclear options. In the analyses, it is assumed that different types of new reactors may be built, taking into account the introduction date of considered Gen-Ill (i.e. EPR) and Gen-IV (i.e. SCWR, HTR, FR) reactors, and the economic evaluation of the complete fuel cycle. The assessment was undertaken using the DANESS code (Dynamic Analysis of Nuclear Energy System Strategies). The analyses show that given the considered realistic nuclear energy demand and given a limited number of available Gen-III and Gen-IV reactor types, the future European nuclear park will exist of combinations of Gen-III and Gen-IV reactors. This mix will always consist of a set of reactor types each having its specific strengths. The analyses also highlight the triggers influencing the choice between different nuclear energy deployment scenarios. (authors)

  16. Early Antibody Lineage Diversification and Independent Limb Maturation Lead to Broad HIV-1 Neutralization Targeting the Env High-Mannose Patch.

    Science.gov (United States)

    MacLeod, Daniel T; Choi, Nancy M; Briney, Bryan; Garces, Fernando; Ver, Lorena S; Landais, Elise; Murrell, Ben; Wrin, Terri; Kilembe, William; Liang, Chi-Hui; Ramos, Alejandra; Bian, Chaoran B; Wickramasinghe, Lalinda; Kong, Leopold; Eren, Kemal; Wu, Chung-Yi; Wong, Chi-Huey; Kosakovsky Pond, Sergei L; Wilson, Ian A; Burton, Dennis R; Poignard, Pascal

    2016-05-17

    The high-mannose patch on HIV Env is a preferred target for broadly neutralizing antibodies (bnAbs), but to date, no vaccination regimen has elicited bnAbs against this region. Here, we present the development of a bnAb lineage targeting the high-mannose patch in an HIV-1 subtype-C-infected donor from sub-Saharan Africa. The Abs first acquired autologous neutralization, then gradually matured to achieve breadth. One Ab neutralized >47% of HIV-1 strains with only ∼11% somatic hypermutation and no insertions or deletions. By sequencing autologous env, we determined key residues that triggered the lineage and participated in Ab-Env coevolution. Next-generation sequencing of the Ab repertoire showed an early expansive diversification of the lineage followed by independent maturation of individual limbs, several of them developing notable breadth and potency. Overall, the findings are encouraging from a vaccine standpoint and suggest immunization strategies mimicking the evolution of the entire high-mannose patch and promoting maturation of multiple diverse Ab pathways. PMID:27192579

  17. Apratylenchus vietnamensis gen. n., sp. n. and A. binhi gen. n., sp. n., sedentary Pratylenchidae (Nematoda:Tylenchida) from coffee in Vietnam, with proposal of Apratylenchinae subfam. n.

    NARCIS (Netherlands)

    Trinh, P.Q.; Waeyenberge, L.; Nguyen, C.N.; Baldwin, J.G.; Karssen, G.; Moens, M.

    2009-01-01

    Apratylenchinae subfam. n. is proposed within the Pratylenchidae to include Apratylenchus gen. n. and two new species, A. vietnamensis gen. n., sp. n. and A. binhi gen. n., sp. n., from coffee plantations in Vietnam. Apratylenchus gen. n. is distinguished by the presence of transverse rows of cuticu

  18. R5 clade C SHIV strains with tier 1 or 2 neutralization sensitivity: tools to dissect env evolution and to develop AIDS vaccines in primate models.

    Directory of Open Access Journals (Sweden)

    Nagadenahalli B Siddappa

    Full Text Available BACKGROUND: HIV-1 clade C (HIV-C predominates worldwide, and anti-HIV-C vaccines are urgently needed. Neutralizing antibody (nAb responses are considered important but have proved difficult to elicit. Although some current immunogens elicit antibodies that neutralize highly neutralization-sensitive (tier 1 HIV strains, most circulating HIVs exhibiting a less sensitive (tier 2 phenotype are not neutralized. Thus, both tier 1 and 2 viruses are needed for vaccine discovery in nonhuman primate models. METHODOLOGY/PRINCIPAL FINDINGS: We constructed a tier 1 simian-human immunodeficiency virus, SHIV-1157ipEL, by inserting an "early," recently transmitted HIV-C env into the SHIV-1157ipd3N4 backbone [1] encoding a "late" form of the same env, which had evolved in a SHIV-infected rhesus monkey (RM with AIDS. SHIV-1157ipEL was rapidly passaged to yield SHIV-1157ipEL-p, which remained exclusively R5-tropic and had a tier 1 phenotype, in contrast to "late" SHIV-1157ipd3N4 (tier 2. After 5 weekly low-dose intrarectal exposures, SHIV-1157ipEL-p systemically infected 16 out of 17 RM with high peak viral RNA loads and depleted gut CD4+ T cells. SHIV-1157ipEL-p and SHIV-1157ipd3N4 env genes diverge mostly in V1/V2. Molecular modeling revealed a possible mechanism for the increased neutralization resistance of SHIV-1157ipd3N4 Env: V2 loops hindering access to the CD4 binding site, shown experimentally with nAb b12. Similar mutations have been linked to decreased neutralization sensitivity in HIV-C strains isolated from humans over time, indicating parallel HIV-C Env evolution in humans and RM. CONCLUSIONS/SIGNIFICANCE: SHIV-1157ipEL-p, the first tier 1 R5 clade C SHIV, and SHIV-1157ipd3N4, its tier 2 counterpart, represent biologically relevant tools for anti-HIV-C vaccine development in primates.

  19. Türk ankilozan spondilitli hastalarda HLA-B27 ve MEFV gen mutasyonları arasındaki ilişki

    OpenAIRE

    SARIKAYA PEKACAR, Filiz

    2015-01-01

    Ankilozan spondilit (AS), patogenezinde bir çok genetik, immunolojik ve çevresel faktörün birlikte rol oynadığı  inflamatuvar bir hastalıktır. Genetik faktörler arasında en büyük pay HLA -B27’nindir. Genom  çapında ilişkilendirme çalışmaları sonucu AS ile ilgili bir çok gen bulunmuş MHC dışı genlerin hastalığın  gelişiminde rol oynadığı düşünülmüştür.
    Bu tez çalışmasında HLA-B27, MEFV gen mutasyonları, IL12B, IL23R ve ERAP1 gen polimorfiz...

  20. Aconselhamento genético Genetic counseling

    Directory of Open Access Journals (Sweden)

    João Monteiro de Pina-Neto

    2008-08-01

    Full Text Available OBJETIVO: Esta revisão sobre aconselhamento genético (AG teve o objetivo de mostrar os conceitos atuais e os princípios filosóficos e éticos aceitos na grande maioria dos países e recomendados pela Organização Mundial da Saúde, as fases do processo, seus resultados e o impacto psicológico de uma doença genética em uma família. FONTES DOS DADOS: Os conceitos apresentados são baseados em uma síntese histórica da literatura sobre AG desde a década de 1930 até o momento atual, sendo que os artigos citados representam os principais trabalhos publicados e que hoje fundamentam a teoria e a prática do AG. SÍNTESE DOS DADOS: O AG modernamente é definido como um processo de comunicação que trata dos problemas humanos relacionados à ocorrência de uma doença genética em uma família. É fundamental que os profissionais da saúde conheçam os aspectos psicológicos desencadeados pela doença genética e como estes aspectos podem ser manejados. Vivemos ainda na genética humana e médica uma fase de predomínio dos aspectos técnicos e científicos e de pouca ênfase no estudo das reações emocionais e dos processos de adaptação das pessoas a estas doenças, o que leva ao baixo entendimento dos clientes sobre os fatos ocorridos, com conseqüências negativas sobre a vida familiar e para a sociedade. CONCLUSÕES: Conclui-se pela necessidade de que as famílias com doenças genéticas sejam encaminhadas para AG e que os profissionais desta área invistam mais na humanização do atendimento, desenvolvendo mais as técnicas do AG psicológico não-diretivo.OBJECTIVE: The objective of this review of genetic counseling (GC is to describe the current concepts and philosophical and ethical principles accepted by the great majority of countries and recommended by the World Health Organization, the stages of the process, its results and the psychological impact that a genetic disease has on a family. SOURCES: The concepts presented are

  1. Marcadores genéticos del bovino criollo : Su germoplasma como recurso genético

    OpenAIRE

    Quinteros, Indalecio Rodolfo

    1994-01-01

    En la presente comunicación se propone continuar en la investigación del germoplasma del ganado bovino por los Marcadores Genéticos Eritrocitarios y Bioquímicos en correspondencia a poblaciones de distintas regiones de Argentina y América, como contribución a otros tipos de estudios

  2. TidGen Power System Commercialization Project

    Energy Technology Data Exchange (ETDEWEB)

    Sauer, Christopher R. [President & CEO; McEntee, Jarlath [VP Engineering & CTO

    2013-12-30

    ORPC Maine, LLC, a wholly-owned subsidiary of Ocean Renewable Power Company, LLC (collectively ORPC), submits this Final Technical Report for the TidGen® Power System Commercialization Project (Project), partially funded by the U.S. Department of Energy (DE-EE0003647). The Project was built and operated in compliance with the Federal Energy Regulatory Commission (FERC) pilot project license (P-12711) and other permits and approvals needed for the Project. This report documents the methodologies, activities and results of the various phases of the Project, including design, engineering, procurement, assembly, installation, operation, licensing, environmental monitoring, retrieval, maintenance and repair. The Project represents a significant achievement for the renewable energy portfolio of the U.S. in general, and for the U.S. marine hydrokinetic (MHK) industry in particular. The stated Project goal was to advance, demonstrate and accelerate deployment and commercialization of ORPC’s tidal-current based hydrokinetic power generation system, including the energy extraction and conversion technology, associated power electronics, and interconnection equipment capable of reliably delivering electricity to the domestic power grid. ORPC achieved this goal by designing, building and operating the TidGen® Power System in 2012 and becoming the first federally licensed hydrokinetic tidal energy project to deliver electricity to a power grid under a power purchase agreement in North America. Located in Cobscook Bay between Eastport and Lubec, Maine, the TidGen® Power System was connected to the Bangor Hydro Electric utility grid at an on-shore station in North Lubec on September 13, 2012. ORPC obtained a FERC pilot project license for the Project on February 12, 2012 and the first Maine Department of Environmental Protection General Permit issued for a tidal energy project on January 31, 2012. In addition, ORPC entered into a 20-year agreement with Bangor Hydro Electric

  3. Genética de la conservación: la aplicación de los conceptos de la evolución a la conservación de la diversidad biológica

    OpenAIRE

    Caballero Rúa, Armando

    2013-01-01

    La comprensión de las fuerzas de cambio evolutivo que actúan sobre las poblaciones, en conjunción con las técnicas más modernas de análisis genético, lleva aplicándose en los últimos veinte años al servicio de la conservación de la biodiversidad. Esta nueva aplicación de la genética ha recibido el nombre de genética de la conservación.

  4. Análisis de la asociación entre sexo y diferencias en pigmentación humana según el genotipo del gen MC1R

    OpenAIRE

    Hernando, Bárbara; Ibarrola-Villava, Maider; Llorca-Cardeñosa, Marta J.; Ribas, Gloria; Martínez-Cadenas, Conrado

    2016-01-01

    Introducción: La pigmentación cutánea basal y la respuesta al sol por bronceamiento son rasgos hereditarios influidos por varios genes, entre los que el gen mc1r es uno de los más importantes. Mutaciones en este gen afectan a los niveles y tipos de me-lanina dando lugar a patrones alterados de pigmentación. Recientemente, se ha iden-tificado una asociación entre el genotipo del gen oca2, el color de ojos y el sexo, su-giriendo que existe un factor relacionado con el sexo que contribuye a las ...

  5. Aconselhamento Genético Genetic Counseling

    Directory of Open Access Journals (Sweden)

    Décio Brunoni

    2002-01-01

    Full Text Available O Aconselhamento Genético (AG deve ser desenvolvido nas unidades de saúde como um atendimento médico multiprofissional e interdisciplinar. A maioria da população brasileira não tem acesso a esses serviços. Os grupos, centros e serviços de AG existentes no Brasil podem, em curto espaço de tempo, preparar profissionais para atender a rede do Sistema Único de Saúde (SUS. O Ministério da Saúde tem os instrumentos necessários para viabilizar esta estratégia. Medidas que visem incrementar o ensino da genética médica nos cursos de graduação e em curso de especialização/aperfeiçoamento podem ser efetivadas. O envolvimento da população, de pacientes e famílias no conhecimento dos fatores genéticos que agravam a saúde é desejável e pode ser alcançado por ações de genética comunitária como tem sido recomendado pela Organização Mundial da Saúde (OMS.Genetic counseling (GC is a medical multiprofessional and interdisciplinar health care process. Most of the Brazilian people don't have access to these services. In Brazil there are many GC groups, centers and services in condition to train health care professionals to the Federal Brazilian Public System of Health (Sistema Único de Saúde - SUS. Ministry of Health could easily created an Office of Genetic Services to organize and launch trainning programs. It is also necessary improve genetic educational programs for the health professions as well as educate the public in genetic disorders and birth defects. Support of parent/patient organizations is also desirable. The implementation of such community genetics programs has been recommended by the World Health Organization (WHO.

  6. Gen IV Materials Handbook Functionalities and Operation

    Energy Technology Data Exchange (ETDEWEB)

    Ren, Weiju [ORNL

    2009-12-01

    This document is prepared for navigation and operation of the Gen IV Materials Handbook, with architecture description and new user access initiation instructions. Development rationale and history of the Handbook is summarized. The major development aspects, architecture, and design principles of the Handbook are briefly introduced to provide an overview of its past evolution and future prospects. Detailed instructions are given with examples for navigating the constructed Handbook components and using the main functionalities. Procedures are provided in a step-by-step fashion for Data Upload Managers to upload reports and data files, as well as for new users to initiate Handbook access.

  7. Genética, performance física humana e doping genético: o senso comum versus a realidade científica

    Directory of Open Access Journals (Sweden)

    Rodrigo Gonçalves Dias

    2011-02-01

    Full Text Available Atletas de elite são reconhecidos como fenômenos esportivos e o potencial para atingir níveis superiores de performance no esporte está parcialmente sob o controle de genes. A excelência atlética é essencialmente multifatorial e determinada por complexas interações entre fatores ambientais e genéticos. Existem aproximadamente 10 milhões de variantes genéticas dispersas por todo o genoma humano e uma parcela destas variantes têm demonstrado influenciar a responsividade ao treinamento físico. Os fenótipos de performance física humana parecem ser altamente poligênicos e alguns estudos têm comprovado a existência de raras combinações genotípicas em atletas. No entanto, os mecanismos pelos quais genes se interagem para amplificar a performance física são desconhecidos. O conhecimento sobre os genes que influenciam a treinabilidade somado ao potencial uso indevido dos avanços da terapia gênica, como a possível introdução de genes em células de atletas, fez surgir o termo doping genético, um novo e censurado método de amplificação da performance física, além dos limites fisiológicos. Aumentos na hipertrofia muscular esquelética e nos níveis de hematócrito estão sendo conseguidos através da manipulação da expressão de genes específicos, mas a grande parte das impressionáveis alterações foi obtida em experimentação com animais de laboratório. A compreensão dos resultados científicos envolvendo genética, performance física humana e doping genético é uma difícil tarefa. Com o propósito de evitar a contínua má interpretação e propagação de conceitos errôneos, esta revisão, intencionalmente, vem discutir as evidências científicas produzidas até o momento sobre o tema, permitindo a compreensão do atual "estado da arte"

  8. Correlaciones fenotípicas, ambientales y genéticas en berenjena

    Directory of Open Access Journals (Sweden)

    Hermes Araméndiz Tatis

    2009-10-01

    Full Text Available En el Centro de Investigaciones Turipaná de la Corporación Colombiana de Investigaciones (Corpoica (Cereté, Córdoba, Colombia -8° 31' N y 75° 58' O, a 13 m.s.n.m se estudiaron las correlaciones, ambientales y genéticas entre seis caracteres de 24 cultivares de berenjena (Solanum melongena. Para el efecto se utilizó un diseño de bloques completamente al azar con tres repeticiones y unidades experimentales de 10 m². Los resultados mostraron que las correlaciones fueron de mayor o igual magnitud que las fenotípicas, mientras que las ambientales fueron de escaso valor. El número de frutos y el rendimiento estuvieron genéticamente correlacionados (r = 0.56, P < 0.01, la longitud y la resistencia del fruto mostraron correlación genética negativa (r = -0.68, P < 0.01 y entre el rendimiento y peso de fruto la correlación fue muy baja (r = 0.04. El número de frutos y su peso de frutos se correlacionaron de manera negativa (r =-0.63, P < 0.01. El número de frutos por planta puede ser utilizado como criterio de selección para la obtención de cultivares de berenjena de altos rendimientos.

  9. Correlaciones fenotípicas, ambientales y genéticas en berenjena

    Directory of Open Access Journals (Sweden)

    Cardona Ayala Carlos Enrique

    2009-12-01

    Full Text Available En el Centro de Investigaciones Turipaná de la Corporación Colombiana de Investigaciones (Corpoica (Cereté, Córdoba, Colombia –8° 31' N y 75° 58' O, a 13 m.s.n.m se estudiaron las correlaciones, ambientales y genéticas entre seis caracteres de 24 cultivares de berenjena (Solanum melongena. Para el efecto se utilizó un diseño de bloques completamente al azar con tres repeticiones y unidades experimentales de 10 m2. Los resultados mostraron que las correlaciones fueron de mayor o igual magnitud que las fenotípicas, mientras que las ambientales fueron de escaso valor. El número de frutos y el rendimiento estuvieron genéticamente correlacionados (r = 0.56, P < 0.01, la longitud y la resistencia del fruto mostraron correlación genética negativa (r = -0.68, P < 0.01 y entre el rendimiento y peso de fruto la correlación fue muy baja (r = 0.04. El número de frutos y su peso de frutos se correlacionaron de manera negativa (r =-0.63, P < 0.01. El número de frutos por planta puede ser utilizado como criterio de selección para la obtención de cultivares de berenjena de altos rendimientos.

  10. El papel del gen del transportador de serotonina en los trastornos de la conducta alimentaria

    Directory of Open Access Journals (Sweden)

    Sandra Hernández-Muñoz

    2014-10-01

    Full Text Available Introducción: Al sistema serotoninérgico se lo ha implicado en la regulación del estado de ánimo y en la conducta alimentaria, por lo que el gen del transportador de serotonina (SLC6A4 es un buen candidato para el desarrollo de los trastornos de la conducta alimentaria (TCA. La mayoría de los estudios genéticos en los TCA se han centrado principalmente en un polimorfismo, el denominado 5-HTTLPR del gen SLC6A4. Objetivo: Realizar una revisión de los estudios de asociación entre el 5-HTTLPR y los TCA, como anorexia nerviosa, bulimia nerviosa y trastornos alimentarios no especificados. Método: Se realizó una búsqueda en MEDLINE, ISI y PubMed de las palabras clave «transportador de serotonina» y «TCA». Conclusiones: Según la revisión de 37 artículos originales, la variante S del 5-HTTLPR es un factor de riesgo de anorexia nerviosa. Además, se encontró asociación entre el alelo S y el índice de masa corporal, impulsividad, ansiedad, depresión y el tiempo de evolución en TCA. Sin embargo, en bulimia nerviosa no se reporta asociación con las variantes del 5-HTTLPR.

  11. Diversidade genética em palmeiras através de isoenzimas e RAPD

    Directory of Open Access Journals (Sweden)

    SAWAZAKI H. E.

    1998-01-01

    Full Text Available Estudou-se, mediante polimorfismo enzimático em gel de poliacrilamida e polimorfismo de DNA com base na amplificação de segmentos de DNA ao acaso, denominado RAPD, a variabilidade genética em algumas espécies e ecótipos de palmeiras dos gêneros Euterpe, Bactris, Elaeis e Syagrus. Os extratos de folhas de mudas dessas palmeiras foram analisados para as isoenzimas de malato desidrogenase (MDH, leucinoaminopeptidase (LAP, glutamato oxaloacetato transaminase (GOT, fosfoglucose isomerase (PGI, fosfoglucose mutase (PGM, fosfatase ácida (ACP, peroxidase (PRX, esterase (EST e para os marcadores RAPD, utilizando os "primers" dos kits A e B da Operon Technologies. Verificou-se grande variabilidade genética interespecífica, comprovada pelos dendrogramas UPGMA, com reconhecimento de híbridos. Foram observadas várias bandas além das referidas pela literatura em gel de amido. Os resultados dos marcadores RAPD comprovaram os das isoenzimas com maior eficácia, pois possibilitaram facilmente a análise de grande número de marcadores genéticos.

  12. Engenharia Genética: busca da mercadoria perfeita?

    OpenAIRE

    José Neivaldo de Souza

    2014-01-01

    Resenha:Engenharia Genética: busca da mercadoria perfeita? -SANDEL, Michael J. Contra a Perfeição: ética na era da engenharia genética. 1ª.ed. Tradução por Ana Carolina Mesquita. Rio de janeiro: Civilização Brasileira, 2013. 160p. 

  13. Hoja informativa de pruebas genéticas

    Science.gov (United States)

    Trata de pruebas genéticas para riesgos heredados de cáncer; incluye tipos de pruebas, para quién son, explicación y confidencialidad de los resultados. Ofrece información de pruebas genéticas a domicilio o pruebas para el consumidor.

  14. Towards an International Culture: Gen Y Students and SNS?

    Science.gov (United States)

    Lichy, Jessica

    2012-01-01

    This article reports the findings of a small-scale investigation into the Internet user behaviour of generation Y (Gen Y) students, with particular reference to social networking sites. The study adds to the literature on cross-cultural Internet user behaviour with specific reference to Gen Y and social networking. It compares how a cohort of…

  15. Ética y genética

    OpenAIRE

    2002-01-01

    Este documento contiene una transcripción de las ponencias presentadas en la Mesa Redonda sobre Ética y Genética celebrada en Oviedo, el día 10 de octubre de 2001, y organizada por el Real Patronato sobre Discapacidad en el marco del XXI Congreso Nacional de Genética Humana.

  16. ALS Association

    Science.gov (United States)

    ... toward a world without ALS! Walk to Defeat ALS® Walk to Defeat ALS® draws people of all ... We need your help. I Will Advocate National ALS Registry The National ALS Registry is a congressionally ...

  17. Thaumetopoea pityocampa i nematodes entomopatògens : un mètode alternatiu de control biològic de la plaga

    OpenAIRE

    Franquet Barrera, Laura; García del Pino, Fernando

    2009-01-01

    L'objectiu principal d'aquesta investigació és determinar la viabilitat dels nematodes entomopatògens per controlar les plagues de Thaumetopoea pityocampa, tenint en compte que presenten tot un seguit de característiques que els fan adequats per al control de diferents plagues d'insectes.

  18. Caracterización genética de la raza chato murciano

    OpenAIRE

    Herrero Medrano, Juan Manuel

    2012-01-01

    El Chato Murciano es una raza local porcina autóctona del sureste de España y en peligro de extinción. Esta raza, al igual que otras muchas razas locales, está amenazada por los efectos de la endogamia y el cruce con otras razas porcinas. En el presente trabajo se utilizaron conjuntamente tres marcadores genéticos, concretamente un panel de 34 microsatélites, un panel de alta densidad de SNPs y un fragmento de ADN mitocondrial que se analizaron en la práctica totalidad de la...

  19. Sèrie Tipologies Comercials. Comerç Just. Part 2. Orígens

    OpenAIRE

    Juan Vigaray, María Dolores de; González Gascón, Elena

    2014-01-01

    En aquest vídeo veurem quina ha estat l'expansió del Comerç Just des dels seus orígens després de la II Guerra Mundial. Des del primer exemple, el 1946 a Estats Units, quan l'organització Ten Thousand Villages va començar a vendre treballs de costura produïts per dones pobres de Puerto Rico, fins al llançament del seu segell actual, juntament amb la marca FTO (Fair Trade Organizations), el 2002.

  20. Vizier: un sistema de recomendación genérico y multidimensional

    OpenAIRE

    Carrillo Ramos, Angela Cristina

    2012-01-01

    Los Sistemas de Recomendación, al ser herramientas que puedan ser utilizadas para identificar fácilmente productos de interés, han surgido para apoyar, aumentar y automatizar el proceso natural y diario de compartir recomendaciones, y de esta manera, reducir un espacio de alternativas. Este artículo tiene como objetivo presentar Vizier, un framework construido bajo un enfoque genérico, que proporciona servicios a aplicaciones de recuperación de información para que éstas a su vez, se encuentr...

  1. Interrupciones de embarazo por causa genética Interruptions of pregnancy through genetic

    OpenAIRE

    Isabel Figueroa Calderón; Daymi Saavedra Moredo; Yudith de la Torres Sieres; Mayra Sánchez Lueiro

    2012-01-01

    Introducción: las malformaciones congénitas incrementan notablemente las tasas de mortalidad infantil y comprometen la calidad de vida del infante. Objetivo: cuantificar las Interrupciones de causa genética y conocer las malformaciones congénitas más frecuentes en la población. Métodos: se realizó un estudio observacional descriptivo en el Hospital Docente Provincial "Ana Betancourt de Mora" de Camagüey, en el período comprendido del 1ro. de enero de 2004 al 31 de diciembre de 2011. El univer...

  2. La figura del autor en el arte genético y transgénico

    OpenAIRE

    Matewecki, Natalia

    2006-01-01

    Las prácticas artísticas contemporáneas redefinen constantemente el estatuto del arte tradicional al tensionar, por ejemplo, los límites entre el arte y la ciencia. El arte genético y el arte transgénico plantean un trabajo multidisciplinario en el que participan artistas y científicos a la vez, dando lugar así a la construcción de nuevas figuras de autor como las de artista-científico, artista-investigador, artista-técnico o científico-artista. La figura de artista se configura, según Dan...

  3. Optimización global con algoritmos genéticos

    OpenAIRE

    Carretero López, Félix

    2010-01-01

    El objetivo principal de este TFC es dar a conocer al lector el mundo de los métodos de optimización global mediante los algoritmos genéticos. Para alcanzar este objetivo empezaremos por exponer las bases teóricas en las que se fundamentan y a continuación las intentaremos corroborar empíricamente mediante un conjunto de pruebas simples. A partir de los resultados y con todo lo aprendido intentaremos resolver un problema real más complejo. En concreto intentaremos equilibrar la carga de las b...

  4. Université de Genève

    CERN Multimedia

    2008-01-01

    Ecole de physique - Département de physique nucléaire et corspusculaire 24, quai Ernest-Ansermet 1211 GENÈVE 4 Tél: (022) 379 62 73 - Fax: (022) 379 69 92 Lundi 1er décembre 2008 PARTICLE PHYSICS SEMINAR at 17.00 hrs – Stückelberg Auditorium Superconducting Interfaces between Insulating Oxide Prof. Jean-Marc TRISCONE / Université de Genève At interfaces between complex oxides, electronic systems with unusual properties can be generated. A striking example is the interface between LaAlO3 and SrTiO3, two good insulating perovskite oxides, which was found in 2004 to be conducting with a high mobility. We recently discovered that the ground state of this system is a superconducting condensate, with a critical temperature of about 200 mK. The characteristics observed for the superconducting transitions are consistent with a two-dimensional superconducting sheet as thin as a few nanometers. Recent field effect experiments revealed the sensitivity of the normal and superconducting states to the carrier d...

  5. Université de Genève

    CERN Multimedia

    2008-01-01

    Ecole de physique - Département de physique nucléaire et corspusculaire 24, quai Ernest-Ansermet - 1211 GENÈVE 4 Tél: (022) 379 62 73 - Fax: (022) 379 69 92 Lundi 1er décembre 2008 PARTICLE PHYSICS SEMINAR at 17.00 hrs – Stückelberg Auditorium Superconducting Interfaces between Insulating Oxide Prof. Jean-Marc TRISCONE / Université de Genève At interfaces between complex oxides, electronic systems with unusual properties can be generated. A striking example is the interface between LaAlO3 and SrTiO3, two good insulating perovskite oxides, which was found in 2004 to be conducting with a high mobility. We recently discovered that the ground state of this system is a superconducting condensate, with a critical temperature of about 200 mK. The characteristics observed for the superconducting transitions are consistent with a two-dimensional superconducting sheet as thin as a few nanometers. Recent field effect experiments revealed the sensitivity of the normal and superconducting states to the carrier ...

  6. Lamotheoxyuris ackerti n. gen., n. comb. (Nematoda: Heteroxynematidae parasite of Neotoma spp. (Rodentia: Muridae Lamotheoxyuris ackerti n. gen., n. comb. (Nematoda: Heteroxynematidae parásito de Neotoma spp. (Rodentia: Muridae

    Directory of Open Access Journals (Sweden)

    JORGE FALCÓN-ORDAZ

    2010-06-01

    Full Text Available On the basis of the revision of the type material of Aspiculuris ackerti Kruidenier & Mehra, 1959, and new specimens collected from Neotoma nelsoni Goldman, 1905 (Rodentia: Cricetidae, in Veracruz, Mexico, we herein to which A. ackerti is transferred as Lamotheoxyuris ackerti This new genus differs from all other genera included in 1 mouth surrounded by six lips; 2 extension of lateral alae describe a new genus (Lamotheoxyuris n. gen., (Kruidener & Mehra, 1959 n. gen., n. comb. Heteroxynematinae by the following main traits: reduced; and 3 lack of caudal alae.Con base en la revisión del material tipo de Aspiculuris ackerti Kruidenier y Mehra, 1959 y de nuevos ejemplares recolectados en Neotoma nelsoni Goldman, 1905 (Rodentia: Cricetidae, en Veracruz, México, se describe un nuevo género (Lamotheoxyuris n. gen., al que A. ackerti es transferido como Lamotheoxyuris ackerti (Kruidener y Mehra, 1959 n. gen., n. comb. Este nuevo género se distingue de todos los demás géneros incluidos en Heteroxynematinae por las siguientes características: 1 presencia de seis labios rodeando la boca; 2 extensión reducida del ala lateral; y 3 carencia de ala caudal.

  7. Telefoninterviews als Datenerhebungsstrategie: Erfahrungen und Empfehlungen

    OpenAIRE

    Burke, Lisa A.; Miller, Monica K.

    2001-01-01

    In dem vorliegenden Beitrag werden konkrete Vorschläge zur Nutzung von Telefoninterviews als Datenerhebungsstrategie gemacht. Unsere Überlegungen sind an all jene adressiert, die telefonische Interviews durchführen (wollen), wobei sie vor allem für diejenigen Forscher(innen) hilfreich sein dürften, die über keine oder nur geringe Erfahrungen mit dieser Erhebungsmethode verfügen. Es werden zahlreiche praktische Empfehlungen gegeben, die üblicherweise in der traditionellen Forschungsliteratur n...

  8. Polimorfismo genético relacionado con la probabilidad de desarrollar asma ocupacional en trabajadores expuestos a isocianatos

    Directory of Open Access Journals (Sweden)

    Gaetano Pepe Betancourt

    2014-03-01

    Full Text Available Introducción: El desarrollo tecnológico ha traído como consecuencia el uso de sustancias químicas potencialmente perjudiciales para la salud de los trabajadores. Particularmente el uso de isocianatos ha resultado en una mayor morbilidad de patología respiratoria, especialmente el asma. Considerando que no todos los trabajadores expuestos desarrollan la enfermedad se ha propuesto un modelo de interacción gen-medioambiental, el cual trata de explicar la predisposición genética que tienen algunos individuos a desarrollar asma ocupacional y otros no. Objetivo: Conocer la evidencia científica relacionada con el polimorfismo genético y la susceptibilidad que tienen los trabajadores expuestos a isocianatos a desarrollar asma ocupacional. Metodología: Se realizó una revisión sistemática mediante una búsqueda bibliográfica utilizando las bases de datos PubMedline, así como en los repositorios Dialnet y ELSEVIER. Se extrajeron los artículos relacionados al objetivo de esta revisión, no se aplicaron filtros de temporalidad, utilizándose los siguientes descriptores: MeSH Major Topic, MeSH Terms. El periodo de búsqueda fue desde el 20 de noviembre de 2013 y finalizó el 15 de diciembre de 2013. El nivel de evidencia se estableció de acuerdo a los criterios GRADE. Resultados: Se analizaron a texto completo 42 artículos, la evidencia científica se sustentó en 11 estudios de casos-controles. Dada la complejidad del polimorfismo genético asociado con la expresión fenotípica de la enfermedad, como limitación de los estudios, los autores coinciden que el tamaño muestral no es suficientemente grande, sin embargo después de ajustar los factores de confusión los artículos encontrados tuvieron un nivel de evidencia B de GRADE. Conclusión: La genética tiene una influencia significativa en el asma ocupacional inducida por isocianatos. El peso de la susceptibilidad genética y de la interacción gen-medioambiente aún no se han

  9. Türkiye'de kültür ve doğal çipura (Sparus aurata l.) populasyonları arasındaki filogenetik farklılıkların mitokondriyal gen bölgelerinin dna dizi analizleri ile tanımlanması

    OpenAIRE

    DOĞANKAYA, Levent

    2010-01-01

      Bu çalışmada, kıyılarımızda bulunan doğal çipura (Sparus aurata)  populasyonları ile balık çiftliklerinde üretilen çipura populasyonlarının filogenetik ilişkilerinin incelenmesi ve bu stoklar arasındaki farklılıkların belirlenmesi amaçlanmıştır.

    Araştırma için Adana, Mersin, Antalya, Bodrum ve İzmir olmak üzere beş istasyon seçilmiştir. Her istasyonda doğal ve kültür balık stoklarından temin edilen örneklerin karaciğerlerinde...

  10. EarthEnv-DEM90: A nearly-global, void-free, multi-scale smoothed, 90m digital elevation model from fused ASTER and SRTM data

    Science.gov (United States)

    Robinson, Natalie; Regetz, James; Guralnick, Robert P.

    2014-01-01

    A variety of DEM products are available to the public at no cost, though all are characterized by trade-offs in spatial coverage, data resolution, and quality. The absence of a high-resolution, high-quality, well-described and vetted, free, global consensus product was the impetus for the creation of a new DEM product described here, 'EarthEnv-DEM90'. This new DEM is a compilation dataset constructed via rigorous techniques by which ASTER GDEM2 and CGIAR-CSI v4.1 products were fused into a quality-enhanced, consistent grid of elevation estimates that spans ∼91% of the globe. EarthEnv-DEM90 was assembled using methods for seamlessly merging input datasets, thoroughly filling voids, and smoothing data irregularities (e.g. those caused by DEM noise) from the approximated surface. The result is a DEM product in which elevational artifacts are strongly mitigated from the input data fusion zone, substantial voids are filled in the northern-most regions of the globe, and the entire DEM exhibits reduced terrain noise. As important as the final product is a well defined methodology, along with new processing techniques and careful attention to final outputs, that extends the value and usability of the work beyond just this single product. Finally, we outline EarthEnv-DEM90 acquisition instructions and metadata availability, so that researchers can obtain this high-resolution, high-quality, nearly-global new DEM product for the study of wide-ranging global phenomena.

  11. Low pH Is Required for Avian Sarcoma and Leukosis Virus Env-Dependent Viral Penetration into the Cytosol and Not for Viral Uncoating

    OpenAIRE

    Barnard, Richard J. O.; Narayan, Shakti; Dornadula, Geethanjali; Miller, Michael D.; Young, John A. T.

    2004-01-01

    A novel entry mechanism has been proposed for the avian sarcoma and leukosis virus (ASLV), whereby interaction with specific cell surface receptors activates or primes the viral envelope glycoprotein (Env), rendering it sensitive to subsequent low-pH-dependent fusion triggering in acidic intracellular organelles. However, ASLV fusion seems to proceed to a lipid mixing stage at neutral pH, leading to the suggestion that low pH might instead be required for a later stage of viral entry such as ...

  12. Estimación de componentes de varianza genética con cruzas autofecundadas de progenitores no endogámicos.

    OpenAIRE

    Jaime Sahagún Castellanos

    2003-01-01

    En el fitomejoramiento genético, la estimación de componentes de la varianza genética frecuentemente requiere hacer cruzas, tarea que se complica por la reducida producción de semilla para evaluación. Para incrementar la cantidad de semilla se ha sugerido el uso de cruzas autofecundadas (CA), pero la teoría de este enfoque se ha restringido al caso, muy limitado, de progenitores homocigóticos con un modelo de dos alelos. Aquí se pretende, para poblaciones panmícticas con multialelismo: 1) Der...

  13. Búsqueda de factores genéticos de susceptibilidad a la artrosis: fenotipos extremos, variantes raras, microsatélites y metaanálisis de genes candidatos

    OpenAIRE

    Rodríguez Fontenla, María Cristina

    2014-01-01

    diversas alteraciones en los tejidos de la articulación. Se trata de una enfermedad compleja de naturaleza multifactorial en la que participan factores sistémicos, biomecánicos y genéticos. El estudio de los factores genéticos implicados en la susceptibilidad a la OA es un campo complejo que ha progresado en los últimos años. En la actualidad se puede hablar de un total de nueve genes y/o loci asociados con OA en población europea al nivel requerido en los GWAS (Genome Wide Associ...

  14. Evaluación del polimorfismo del gen leptina en bovinos en el sistema doble proposito en Chiapas, México.

    OpenAIRE

    Ortiz Salazar, Jorge Alberto

    2011-01-01

    La leptina es una hormona proteica de 16 KDa, compuesta de 146 aminoácidos y es sintetizada principalmente por el tejido adiposo. En el eje hipotálamo-hipófisisgonadal, la leptina juega un papel muy importante en la regulación de la reproducción de los mamíferos. La mutación del gen leptina TT está asociado con la calidad de la carne y leche en bovinos. El objetivo de este estudio fue estimar las frecuencias genotípicas y alélicas del polimorfismo (SNP, Single Nucleotide Polymorphism) del gen...

  15. Diversidad y estructura genética de accesiones de palma de aceite (Elaeis guineesis Jacq.) provenientes de Camerún

    OpenAIRE

    Jose Estiben Pacheco Diaz; Diana Marcela Arias Moreno; Zaida Zarely Ojeda Perez; Hernán Mauricio Romero Angulo

    2014-01-01

    Título en español: Diversidad y estructura genética de accesiones de palma de aceite (Elaeis guineesis Jacq.) provenientes de CamerúnTítulo en ingles: Diversity and genetic structure of oil palm accession (Elaeis guineesis Jacq.)  from CameroonTítulo corto: Diversidad genética de accesiones de palma de aceiteResumen: La palma de aceite Elaeis guineesis Jacq. posee gran importancia debido al aceite que se extrae de sus frutos, del cual se obtienen derivados refinados de gran valor comercial co...

  16. Clonación artificial de un controlador on-line, basado en lógica difusa y algoritmos genéticos

    OpenAIRE

    Javier Ballesteros; Alonso Guevara

    2006-01-01

    Los Algoritmos Genéticos son procedimientos adaptativos para la búsqueda de soluciones en espacios complejos, inspirados en la evolución biológica, con patrones de operaciones basados en el principio darwiniano de reproducción y supervivencia de los individuos que mejor se adaptan al entorno en que viven. En este artículo se presenta un estudio sobre los Algoritmos Genéticos y la Lógica Difusa, para desarrollar una metodología propuesta y replicar la caja negra de un controlador, utilizando p...

  17. The GenDev Curriculum Development Workshop.

    Science.gov (United States)

    D'cunha, J

    1997-01-01

    This article describes the second Curriculum Development Workshop held in May 1997 at the Asian Institute of Technology (AIT) in Bangkok, Thailand. The workshop aimed to review critically and restructure the Gender and Development Studies (GenDev) curriculum and to assess AIT's role in training gender experts for the region. Participants included 22 people from 16 countries in Asia, Europe, and the US who were teaching graduate students about gender issues and who were activists with nongovernmental organizations working on gender issues. It was determined that the following were required courses: Culture, Knowledge and Gender Relations; Gender, Technology, and Development; Principles of Gender Research and Methodology in Science and Technology; and Gender Analysis and Field Methods. Other suggested core courses included: Gender and Natural Resource Management; Enterprise Management, Technology, and Gender; Gender and Agrarian Reform; Urbanization: A Gender Perspective; Gender-Responsive Development Planning; and Gender and Economic Change: Past and Present Concerns. Participants distinguished between GenDev courses offered to anyone attending AIT and training courses designed to produce gender experts in the region. The aim of training courses for AIT graduate students was to sensitize potential managers, technologists, and others on gender issues and to create awareness of the importance of including gender perspectives within decision-making, policy formation, and implementation. Training courses to produce gender experts should be directed to those with a prior background in gender studies and include gender analysis in field methods. Participants agreed that there should be an independent and autonomous field of gender and development studies. Participants made six recommendations for such a field of study. PMID:12179927

  18. Aplicación de la Genética a la Cría de las Plantas.

    Directory of Open Access Journals (Sweden)

    Hutchinson J. B.

    1949-09-01

    Full Text Available La influencia de la ciencia de la genética en el arte de la cría de plantas ha sido mucho menos profunda de cuanto esperaban los primeros genetistas. Al dilucidar el mecanismo hereditario, fueron utilizados únicamente factores que originaban grandes diferencias, sin caer en la cuenta inmediata de que ellos son de pequeña importancia naturalmente o bajo la selección artificial. El progreso en el control genético de las pequeñas diferencias ha sido difícil y lento, considerando todavía muchos genetistas que los "genes" de menor categoría no valen la pena de ser investigados. Algunos principios genéticos han sido aplicados con éxito por los criadores, pero, a menudo, los intrincados principios no son totalmente comprendidos y la aplicaci6n se ha hecho con frecuencia sin apoyo experimental, basando las deducciones en observaciones generales sobre el material de cría. En relación con los métodos de cría es notable la frecuencia con que una práctica de fundamental importancia se justifica por una declaración que principia así: "Mi impresión es . .. ". Será tarea del genetista substituir la intuición del criador, por la evidencia objetiva. El objeto de este escrito consiste en revisar la aplicación de la teoría genética al cultivo del algodón en particular y bosquejar los problemas en los cuales el genetista podrá ayudar al criador.

  19. Sobre el significado del descubrimiento del gen FOXP2

    OpenAIRE

    Víctor Manuel LONGA MARTÍNEZ

    2006-01-01

    El reciente descubrimiento del gen FOXP2 ha ofrecido la primera evidencia clara de la base genética del lenguaje, mostrando una correlación inequívoca desde la perspectiva genética entre una versión mutada de F0XP2 y los trastornos lingüísticos de diferente tipo sufridos por una familia inglesa, conocida como KE. El objetivo central del presente trabajo es discutir diferentes aspectos relacionados con tal descubrimiento; especialmente, la discusión del significado de FOXP2 con ...

  20. Estudio de bloques constructivos en algoritmos genéticos

    OpenAIRE

    Murias Rodríguez, Angel

    2007-01-01

    Estudio experimental sobre el efecto de las modificaciones en los bloques constructivos. Para conseguirlo, primero se ha diseñado un algoritmo genético genérico con posibilidad de adaptarlo a un problema específico (función de objetivo a optimizar) e incorporar fácilmente diferentes características que pueden tener estos algoritmos. Como referencia de comparación se ha diseñado y implementado un algoritmo genético estándar y los algoritmos con las modificaciones. Se han probado los algorit...

  1. Intención de compra de medicamentos genéricos por parte de los usuarios de Asturias

    Directory of Open Access Journals (Sweden)

    González Hernando Santiago

    2003-01-01

    Full Text Available Fundamento: Conocer las percepciones de los consumidores acerca del riesgo asociado al uso de medicamentos genéricos y los factores que más influyen en la intención de solicitar un genérico al médico (prescriptor y/o al farmacéutico, a fin de determinar posibles barreras o frenos a la aceptación de los mismos y obtener información que apoye la toma de decisiones de los gestores sanitarios. Métodos: Estudio sobre utilización de medicamentos centrado en la disposición de los pacientes a solicitar una EFG. En esta investigación transversal cuantitativa se entrevistó personalmente a 542 individuos, a la salida de un centro de salud o de un establecimiento de farmacia en Asturias. En el cuestionario se incluía una escala de medición del riesgo percibido en la compra de un medicamento con 15 atributos agrupados en cinco dimensiones. Asimismo se recogió información sobre la intención de consumir medicamentos genéricos y sobre las características demográficas y socioeconómicas de los entrevistados. Para el análisis de los resultados se aplicaron un análisis factorial confirmatorio, regresión múltiple y análisis univariable. El tratamiento de los datos se efectuó con los programas estadísticos EQS y SPSS. Resultados: Percepción media del riesgo (escalas de 1 a 7: funcional: 2,75; físico: 2,68; financiero: 2,19; psicológico: 1,99; social: 1,42. Factores influyentes sobre la intención de solicitar genéricos al médico: riesgo psicológico (p=0,000. Sobre la solicitud al farmacéutico: riesgo psicológico (p=0,000 y riesgo social (p=0,020. Conclusiones: Los agentes interesados en el desarrollo en el mercado de las EFG deben mantener sus esfuerzos de comunicación hacia la equiparación de los aspectos funcionales y financieros entre especialidades del fabricante y especialidades genéricas, pero no deben dejar de lado aspectos psicológicos y sociales del comportamiento de compra del consumidor.

  2. Uso de algoritmos genéticos para detección de objetos en tiempo real

    OpenAIRE

    Martínez Gómez, Jesús; Gómez Martín, José Antonio; García Varea, Ismael; Matellán Olivera, Vicente

    2009-01-01

    El artículo presenta un sistema de Visión para la detección de objetos mediante el uso de algoritmos genéticos. La principal aportación del mismo consiste en la adaptación al tiempo real de algoritmos generalmente utilizados offline. Para conseguir esto se necesita una ejecución eficiente que reduzca al máximo la complejidad del algoritmo. El ámbito de aplicación del sistema es el campeonato de fútbol robótico RoboCup, en concreto la categoría Standard Platform, y debe permi...

  3. Clonación artificial de un controlador on-line, basado en lógica difusa y algoritmos genéticos

    Directory of Open Access Journals (Sweden)

    Javier Ballesteros

    2006-10-01

    Full Text Available Los Algoritmos Genéticos son procedimientos adaptativos para la búsqueda de soluciones en espacios complejos, inspirados en la evolución biológica, con patrones de operaciones basados en el principio darwiniano de reproducción y supervivencia de los individuos que mejor se adaptan al entorno en que viven. En este artículo se presenta un estudio sobre los Algoritmos Genéticos y la Lógica Difusa, para desarrollar una metodología propuesta y replicar la caja negra de un controlador, utilizando procedimientos de obtención del conjunto de reglas de inferencia, agrupamiento difuso y después aplicar el desarrollo del algoritmo genético simple con algunas alteraciones, buscando el objetivo del trabajo propuesto.

  4. Comportamento de dois genótipos de milho cultivados em sistema de aléias preestabelecido com diferentes leguminosas arbóreas Behaviour of two maize genotypes grown in alley cropping system pre-established with diferents leguminous trees

    OpenAIRE

    Andréia Araújo Lima Leite; Altamiro Souza de Lima Ferraz Junior; Emanoel Gomes de Moura; Alana das Chagas Ferreira Aguiar

    2008-01-01

    O cultivo em aléias tem sido recomendado como alternativa para a substituição da agricultura de corte e queima, no trópico úmido, devido à grande capacidade de produção de matéria orgânica e de reciclagem de nutrientes, mas algumas dúvidas quanto à sustentabilidade e à competição interespecífica são persistentes. O objetivo no trabalho foi avaliar a viabilidade da cultura do milho em um sistema de cultivo em aléias de leguminosas arbóreas. O delineamento experimental utilizado foi em blocos c...

  5. Desempenho de genótipos de batata-doce submetidos ao efeito da calagem em Rio Largo-Alagoas

    Directory of Open Access Journals (Sweden)

    Islan Diego Espindula de Carvalho

    2015-09-01

    Full Text Available O objetivo desse trabalho foi avaliar o desempenho de genótipos de batata-doce submetidos ao efeito da calagem em Rio Largo-AL. O delineamento experimental utilizado foi o de blocos casualizados no esquema fatorial (7 x 2,  com sete genótipos de batata-doce e dois tipos de correção do solo, em três repetições. As variáveis avaliadas foram: Número de Raízes Comerciais (NRC; Número Total de Raízes (NTR; Comprimento de Raízes Comerciais (CRC; Diâmetro de Raízes Comerciais (DRC; Rendimento de Raízes Comerciais (RRC; Rendimento Total de Raízes (RTR e Peso Médio de Raízes Comerciais (PMRC. Dentre os genótipos de batata-doce avaliados no experimento, o Clone-06 apresentou o melhor desempenho, superando todos os genótipos avaliados, inclusive as testemunhas, Rainha de Penedo e Sergipana, tanto em produtividade, com rendimento médio de 15,79 t.ha-1de raízes comerciais, quanto em qualidade, apresentando dimensões de raízes comerciais dentro dos padrões de comercialização como Extra A. O desempenho dos genótipos, bem como a grande maioria das variáveis de batata-doce não foram influenciadas pela correção do solo.

  6. Environmental impacts of consumers' choice of food products and housing. Final report of the ConsEnv project; Aterioiden ja asumisen valinnat kulutuksen ympaeristoevaikutusten ytimessae. ConsEnv-hankkeen loppuraportti

    Energy Technology Data Exchange (ETDEWEB)

    Saarinen, M.; Kurppa, S.; Nissinen, A.; Maekelae, J. (eds.)

    2011-06-15

    In the ConsEnv project, climate and eutrophication impacts of food consumption were assessed, and different everyday means of mitigating climate impacts of housing were studied. The life-cycle models that were used for the production of primary raw materials corresponded to average Finnish production, with the exception of imported food products, for which LCA-based specific data from the literature were used. When assessing environmental impacts of the food industry and food commerce, the study evaluated data received from specific companies (Saarioinen, HK Ruokatalo, Fazer Bakeries, Raisio and Ruokakesko). The models used for school meals were based on data received from the kitchen supplying food to the school involved in the study (Kauriala, Haemeenlinna). The impact assessments of consumers' own behaviour, that is, purchasing of food, and food storage and preparation were based on previously published data. When estimating electricity and heat consumption in the food chain, the average profile of Finnish energy production was used. According to the results, animal-based meals have a two- to three-fold climate change impact and a four- to fivefold eutrophication impact, compared to vegetarian dishes. The results also show differences between different meat and vegetable meals. In most cases, the main food ingredient of the meal has the greatest environmental impact. For some meals, the proportion of the impact of salad can be as high as one-third of the total. The relatively higher impact of rice in comparison with that of pasta is not decisive, when the total impact of the meal is taken into account. The differences between home-cooked and convenience food are mainly due to different types of raw materials used. The way of cooking and the anticipated benefits of central kitchens are not as dominant as expected in regard to climate change. The main conclusion from the study is that the greatest source of impacts of different types of meals is the

  7. Intercompartmental Recombination of HIV-1 Contributes to env Intrahost Diversity and Modulates Viral Tropism and Sensitivity to Entry Inhibitors▿†‡

    Science.gov (United States)

    Brown, Richard J. P.; Peters, Paul J.; Caron, Catherine; Gonzalez-Perez, Maria Paz; Stones, Leanne; Ankghuambom, Chiambah; Pondei, Kemebradikumo; McClure, C. Patrick; Alemnji, George; Taylor, Stephen; Sharp, Paul M.; Clapham, Paul R.; Ball, Jonathan K.

    2011-01-01

    HIV-1 circulates within an infected host as a genetically heterogeneous viral population. Viral intrahost diversity is shaped by substitutional evolution and recombination. Although many studies have speculated that recombination could have a significant impact on viral phenotype, this has never been definitively demonstrated. We report here phylogenetic and subsequent phenotypic analyses of envelope genes obtained from HIV-1 populations present in different anatomical compartments. Assessment of env compartmentalization from immunologically discrete tissues was assessed utilizing a single genome amplification approach, minimizing in vitro-generated artifacts. Genetic compartmentalization of variants was frequently observed. In addition, multiple incidences of intercompartment recombination, presumably facilitated by low-level migration of virus or infected cells between different anatomic sites and coinfection of susceptible cells by genetically divergent strains, were identified. These analyses demonstrate that intercompartment recombination is a fundamental evolutionary mechanism that helps to shape HIV-1 env intrahost diversity in natural infection. Analysis of the phenotypic consequences of these recombination events showed that genetic compartmentalization often correlates with phenotypic compartmentalization and that intercompartment recombination results in phenotype modulation. This represents definitive proof that recombination can generate novel combinations of phenotypic traits which differ subtly from those of parental strains, an important phenomenon that may have an impact on antiviral therapy and contribute to HIV-1 persistence in vivo. PMID:21471230

  8. GenBank blastx search result: AK243655 [KOME

    Lifescience Database Archive (English)

    Full Text Available putative transcriptional activator; similar to cAMP-receptor (CAP, CRP) protein: PIR Access...ion Number A26049; similar to fnr (nirR) gene product encoded by GenBank Accession Number J01608;

  9. GenBank blastx search result: AK062102 [KOME

    Lifescience Database Archive (English)

    Full Text Available transcriptional activator; similar to cAMP-receptor (CAP, CRP) protein: PIR Access...ion Number A26049; similar to fnr (nirR) gene product encoded by GenBank Accession Number J01608; aspartic

  10. GenBank blastx search result: AK242621 [KOME

    Lifescience Database Archive (English)

    Full Text Available putative transcriptional activator; similar to cAMP-receptor (CAP, CRP) protein: PIR Access...ion Number A26049; similar to fnr (nirR) gene product encoded by GenBank Accession Number J01608;

  11. 生防荧光假单胞菌2 P24中EnvZ/OmpR双因子系统克隆与OmpR原核表达%Cloning of EnvZ/OmpR Two-component System from Pseudomonas fluorescens 2 P24 and Prokaryotic Expression of OmpR

    Institute of Scientific and Technical Information of China (English)

    孙冰冰; 张伟; 段红英; 李伟; 田涛; 张力群

    2014-01-01

    用Mini-Tn5转座子随机突变荧光假单胞菌野生型菌株2P24,得到抗生素2,4二乙酰基间苯三酚(2,4-di-acetylphloroglucinol,2,4-DAPG)产量提高的突变体Sesu-25。对转座子侧翼序列分析表明,转座子插入到EnvZ/OmpR双因子系统的反应调节因子ompR基因。克隆得到包含完整EnvZ/OmpR系统,全长约为5.9 kb的DNA片段。该双因子系统中的envZ基因和ompR基因与P. fluorescens F113的envZ基因和ompR基因同源性分别为93%和96%。将ompR基因克隆到表达载体pET-22b(+)中,得到重组表达载体pET-ompR。将pET-ompR转入Escherichia coli BL21中得到重组菌株BL21-ompR,用IPTG诱导表达和亲和柱层析法纯化的OmpR蛋白,经SDS-PAGE电泳分析表明ompR基因得到成功表达和纯化。%Using the colour of colony as the selective marker, the random mutagenesis of mini-Tn5 was per-formed to wild-type biocontrol strain Pseudomonas fluorescens 2P24 for the screening of mutants,which might have altered yields of polyketide metabolite 2,4-diacetylphloroglucinol(2,4-DAPG). The strain Sesu-25 with a increased yield of 2,4-DAPG was screened from the mutant pool. The analysis of flank sequence of transposon implied that the response regulator OmpR of EnvZ/OmpR two-component regulatory system ( TCS ) was disrupted in mutant strain Sesu-25. A DNA fragment harbouring a intact EnvZ/OmpR TCS was obtained by subcloning. The envZ and ompR of P. fluorescens 2P24 shared a identity of 93% and 96% with P. fluorescens F113,respectively. The ompR was ligated into the expression vector pET-22b( +) to obtain the recombinated plasmid pET-ompR,and the plasmid pET-ompR was transformed into E. coli BL21 to generate the strain BL21-ompR. The His-taged OmpR was purified by affinity chromatography and verified by SDS-PAGE electrophoresis analysis.

  12. ANALISIS MOLEKULER GEN OMP LEPTOSPIRA UNTUK DIAGNOSIS SEROLOGIS SPESIFIK

    OpenAIRE

    Rosdiana Natzir; Zaraswati Dwyana

    2007-01-01

    Leptospira dapat menginfeksi ginjal, hati, paru-paru, otak dan mata, bahkan dapat menyebabkan kematian, hal ini sering terjadi, bila diagnose dan penanganannya tidak tepat. Dalam pengembangan untuk pencarian terget yang penting untuk membuat serodiagnose terhadap Leptospira, gen OMP merupakan target ideal, dan perlu pengetahuan mengenai gen OMP ini. Pada penelitian ini dilakukan ekstraksi DNA dengan menggunakan Wizard Genomic DNA Purification Kit dari Promega. Setelah itu dilakukan amplif...

  13. Diversidad y diferenciación genética de la yuca ( Manihot esculenta Crantz con marcadores microsatélites en poblaciones de África y Latinoamérica

    Directory of Open Access Journals (Sweden)

    Fregene Martin

    2004-12-01

    Full Text Available Se estudió la diversidad y diferenciación genética de 224 accesiones de yuca tradicionalmente cultivadas en Uganda. Adicionalmente, se incluyeron estudios previos de diversidad, 20 materiales de Tanzania, 20 de Ghana, 22 de Nigeria, 20 de Guatemala y 12 accesiones representando la colección núcleo de Latinoamérica, mantenidas en CIAT. Nueve grupos basados en el país de origen fueron creados para estudiar la variación genética dentro y entre países. Usando secuencias simples repetidas (SSR o marcadores microsatélites, la variación en las frecuencias alélicas en 35 loci no ligados sirvió para estimar los parámetros de diversidad y diferenciación genética. Los resultados afirman una divergencia genética entre accesiones africanas y latinoamericanas, y una fuerte diferenciación de algunas accesiones de Guatemala con respecto a los
    otros países. Ellos también muestran una alta diversidad genética dentro de países y una moderada diferenciación entre ellos. En particular Uganda mantiene alta diversidad genética
    dentro Distritos aún después de una reciente epidemia de CMD (cassava mosaic disease pero baja diferenciación entre ellos. Se discuten las posibles fuerzas implicadas en la dinámica de la diversidad genética, la importancia de Guatemala en los programas de mejoramiento de yuca en la búsqueda de grupos con potencial heterótico, el bajo impacto causado por CMD en la constitución genética del cultivo en Uganda y la observación de una distribución continua de la diversidad genética.

  14. New sequestrate fungi from Guyana: Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. (Boletaceae, Boletales).

    Science.gov (United States)

    Smith, Matthew E; Amses, Kevin R; Elliott, Todd F; Obase, Keisuke; Aime, M Catherine; Henkel, Terry W

    2015-12-01

    Jimtrappea guyanensis gen. sp. nov., Castellanea pakaraimophila gen. sp. nov., and Costatisporus cyanescens gen. sp. nov. are described as new to science. These sequestrate, hypogeous fungi were collected in Guyana under closed canopy tropical forests in association with ectomycorrhizal (ECM) host tree genera Dicymbe (Fabaceae subfam. Caesalpinioideae), Aldina (Fabaceae subfam. Papilionoideae), and Pakaraimaea (Dipterocarpaceae). Molecular data place these fungi in Boletaceae (Boletales, Agaricomycetes, Basidiomycota) and inform their relationships to other known epigeous and sequestrate taxa within that family. Macro- and micromorphological characters, habitat, and multi-locus DNA sequence data are provided for each new taxon. Unique morphological features and a molecular phylogenetic analysis of 185 taxa across the order Boletales justify the recognition of the three new genera. PMID:26732137

  15. Identificación del gen MALT1 como oncogén dominante en el linfoma MALT.

    OpenAIRE

    Sánchez Izquierdo, Maria Dolores

    2005-01-01

    RESUMEN En los linfomas no Hodgkin (LNH), las traslocaciones primarias características dan lugar a la desregulación de un protooncogén (debido en un gran número de casos al reordenamiento con el gen de las inmunoglobulinas) y cuya consecuencia funcional es la alteración de la expresión génica con potencial neoplásico. Otro tipo de lesión genética frecuente es la delección cromosómica que inactiva genes supresores tumorales, por ejemplo p53 ó p16. Estas anomalías se identifican junto a las ...

  16. Reflexión bioética sobre el uso de organismos genéticamente modificados

    Science.gov (United States)

    Yunta, Eduardo Rodríguez

    2011-01-01

    El presente artículo reflexiona desde los 4 principios de la bioética el uso comercial de organismos genéticamente modificados. Se cuestiona fundamentalmente la falta de transferencia de tecnología entre el mundo desarrollado y en desarrollo y el que el presente sistema de patentamiento de organismos vivos modificados fomenta intereses comerciales y no da debida importancia al desarrollo sostenible de la agricultura y ganadería en los países en desarrollo, donde más se necesita. Se reflexiona sobre la importancia que tiene evaluar los riesgos antes de introducirse en el mercado organismos genéticamente modificados y la necesidad de regulación en los países. PMID:21927675

  17. Banque Cantonale de Genève

    CERN Document Server

    Banque Cantonale de Genève

    2011-01-01

    7e Salon Immobilier BCGE le samedi 3 septembre 2011, de 8 h 30 à 13 h 00, au Centre de formation de Conches À cette occasion, les meilleurs spécialistes professionnels genevois de l’immobilier seront réunis en un seul et même lieu. Si vous le souhaitez, un conseiller spécialisé dans les financements hypothécaires évaluera vos possibilités d’investissement immobilier adaptées à votre situation personnelle. En parallèle, les plus importantes régies immobilières de Genève seront à votre disposition pour vous présenter leurs offres actuelles, ainsi que les projets immobiliers futurs et discuter avec vous de la meilleure stratégie à adopter pour trouver l’objet de vos rêves. De plus, vous aurez la possibilité...

  18. Variabilidad genética en cepas de Plasmodium falciparum circulantes en regiones colombianas con riesgo diferente para malaria

    Directory of Open Access Journals (Sweden)

    Mauricio Arcos Burgos

    2000-02-01

    grado de variabilidad genética de la población de P. falciparum circulante en estas regiones se utilizarán como marcadores genéticos los genes que codifican para las proteínas superficiales del merozoito 1 y 2 (MSP-1 y MSP-2 y para la proteína rica en glutamato (GLURP. Por medio de una PCR anidada se amplificarán segmentos altamente polimórficos de estos genes; variantes de las familias alélicas de MSP-1 (MAD20, K1 y RO33 y MSP-2 (FC27 e IC se amplificarán en la segunda reacción. Los productos de la PCR se analizarán por electroforesis en geles de agarosa para determinar el número de alelos para cada gen y la presencia de infección multiclonal.

  19. Construction and characterization of a new simian/human immunodeficiency viruses clone carrying an env gene derived from a CRF07_BC strain

    Institute of Scientific and Technical Information of China (English)

    LI Yue; YANG Gui-bo; CHEN Qi-min; LIU Qiang; MENG Zhe-feng; GENG Yun-qi; QIAO Wen-tao; SHAO Yi-ming

    2009-01-01

    Background The CRF07_BC recombinant strain has been one of the most predominantly circulated HIV-1 strains in China, it is therefore necessary and urgent to develop a relevant animal model to evaluate candidate vaccines targeting HIV-1 CRF07_BC. A highly replication-competent simian/human immunodeficiency viruses (SHIV) construct containing the Chinese CRF07_BC HIV-1 env gene with the ability to infect Chinese rhesus monkeys would serve as an important tool in the development of HIV vaccines. The aim of this study was to examine whether SHIV XJDC6431 with the env fragment from a Chinese HIV-1 isolate virus could infect the human and monkey peripheral blood mononuclear cell (PBMC), establish infection in Chinese rhesus macaque.Methods A SHIV strain was constructed by replacing the rev/env genes of SHIV KB9 with the corresponding fragment derived from the HIV-1 CRF07_BC strain. The infectious activity of the SHIV clones was determined in vitro in PBMCs from both non-human primate animals and humans. Finally, one Chinese rhesus macaques (Macaca mulatto) was infected with one SHIV via intravenous infusion.Results One SHIV clone designated as SHIV XJDC6431, was generated that could infect macaque and human PBMC. The virus produced from this clone also efficiently infected the CCR5-expressing GHOST cell lines, indicating that it uses CCR5 as its coreceptor. Finally, the virus was intravenously inoculated into one Chinese rhesus macaque. Eventually, the animal became infected as shown by the occurrence of viremia within 3 of infection. The viral load reached 10~5 copies of viral RNA per ml of plasma during the acute phase of infection and lasted for 10 weeks post infection. Conclusions We conclude that SHIV XJDC6431 is an R5-tropic chimeric virus, which can establish infection not only in vitro but also in vivo in the Chinese rhesus macaque. Although the animal inoculated with SHIV XJDC6431 became infected without developing a pathologic phenotype, the virus efficiently

  20. Productividad acumulada y su relación genética con características reproductivas en hembras Brahman

    Directory of Open Access Journals (Sweden)

    Orlando Duitama C.

    2013-10-01

    Full Text Available Objetivo. Estimar parámetros genéticos para la característica de productividad acumulada (PAC y correlaciones genéticas con edad al primer parto (EPP, intervalo entre el primero y segundo parto (IEP1 y longevidad (LONG. Materiales y métodos. Fueron usados 8584 registros de hembras Brahman, utilizando un modelo animal en análisis multi-característico con el método de máxima verosimilitud restricta, implementado en el software WOMBAT. Los modelos consideraron los efectos fijos de grupo contemporáneo, número de partos, y la covariable peso al destete del primer ternero; el único efecto aleatorio fue el genético aditivo directo. El peso al destete (P240 fue incluido para disminuir el efecto de la selección en la estimación de los componentes de varianza. Resultados. Las estimativas de heredabilidad fueron de 0.3±0.04, 0.11±0.03, 0.07±0.03 y 0.24±0.04 para EPP, IEP1, LONG y PAC respectivamente. Las correlaciones entre PAC y las otras características se presentaron de moderadas a altas y en sentido favorable. Conclusiones. PAC puede ser incluida en los programas de mejoramiento genético para Brahman, y utilizada como criterio de selección por su heredabilidad moderada y correlación genética favorable con las características reproductivas en estudio.

  1. Caracterización de nuevos reguladores de los mecanismos de tolerancia al daño en el DNA en Saccharomyces cerevisiae

    OpenAIRE

    Gallego Sánchez, Alfonso

    2014-01-01

    [ES]Tanto los mecanismos de tolerancia al daño en el DNA como los checkpoints de integridad del material genético son sistemas esenciales en el mantenimiento de la estabilidad genómica en los organismos eucariotas, concepto este a su vez ligado con campos de estudio tan diversos como la oncogénesis o la evolución de las especies. Empleando la levadura de gemación, Saccharomyces cerevisiae, este trabajo de tesis doctoral ha contribuido, en primer lugar y mediante una aproximación genética, al ...

  2. Diversidad genética y filogenia molecular de poblaciones de Mauritia flexuosa L.f. “aguaje” de la Amazonía Peruana

    Directory of Open Access Journals (Sweden)

    Jorge Angulo-Quintanilla

    2014-07-01

    Full Text Available Mauritia flexuosa es una especie vegetal amazónica que forma extensas poblaciones denominadas “aguajales”. Como los pobladores amazónicos emplean varios órganos de M. flexuosa para suplir sus necesidades y con fines comerciales, se está ejerciendo un gran impacto negativo sobre esta especie. A pesar de ello, a la fecha no se conoce la diversidad genética de esta especie en la Amazonía peruana. Consecuentemente, los planes de manejo para la especie serían limitados sin este tipo de información. Por tanto, el objetivo de esta investigación fue determinar la diversidad genética y filogenia molecular de poblaciones de M. flexuosa aledañas a la carretera Iquitos-Nauta. Las hojas se colectaron en seis zonas contiguas a la carretera Iquitos–Nauta. El ADN purificado con protocolos estándares fue amplificado mediante la técnica de ADN Polimórfico Amplificado al Azar (RAPD con dos cebadores aleatorios.  En total se generaron 28 amplicones RAPD (26 polimórficos y 2 monomórficos. Dentro de los aguajales la diversidad genética fue tres veces mayor (75±19 % que la diversidad genética entre las seis poblaciones de M. flexuosa (25±19 %. La diferenciación genética entre las poblaciones varió de 0,0 a 0,6. Los aguajales que se agruparon en clados en el dendrograma por su mayor similitud genética tuvieron proximidad geográfica. La similitud genética entre las poblaciones de M. flexuosa depende de la distancia geográfica, de tal manera que las poblaciones con más similitud genética están más próximas entre sí que las que tienen menos similitud genética.

  3. Diversidad genética de la población colombiana de ganado Cebú Brahman Americano Bos Indicus (Bovidae

    Directory of Open Access Journals (Sweden)

    Novoa Bravo Miguel Adriano

    2006-12-01

    Full Text Available La raza Cebú Brahman Americano se encuentra en Colombia alrededor de 100 años. Todo ese tiempo, esta raza ha estado bajo un proceso continuo de selección artificial dirigida, reproducción endogámica, efectos de deriva genética causados por eventos fundadores, migraciones de ejemplares entre las fincas del país y animales importados desde otros países. Estos hechos hacen a esta raza interesante y particular desde el punto de vista de la genética de poblaciones. El objetivo de este trabajo es estudiar la estructura y diversidad genética de la raza Cebú Brahman americano. Se utilizaron 162 animales registrados en la asociación colombiana de criadores de ganado cebú (ASOCEBU de 20 departamentos de Colombia. La genotipificación de los animales se llevó a cabo con el kit StockMarks® for cattle bovine genotyping de Applied Biosystems®, empleando 10 microsatélites dinucleótidos. Los resultados de los distintos análisis multivariados (Análisis de componentes principales y análisis de correspondencias múltiples, de inferencia bayesiana y distancias genéticas interindividuales, demuestran que no se presenta subestructura en la población, lo cual se explica por una alta tasa de migración de animales entre las diferentes fincas y regiones, que homogeniza las frecuencias en todo el país. Además, esta población posee un alto grado de heterocigocidad y diversidad alélica, comparado con otras razas, lo cual refleja su origen de mezcla multiracial. También se encontraron diferencias genéticas entre sexos, lo cual es causado por un proceso reproductivo diferencial, donde actúan diferentes criterios de selección entre sexos. Finalmente, al realizar un análisis de componentes principales para analizar las relaciones genéticas de Cebú Brahman americano colombiano con las razas cebuinas y taurinas, se determinó que esta raza se diferencia genéticamente de las demás razas cebuinas, debido a un aporte genético de razas taurinas

  4. Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] lytica to Cellulophaga lytica gen. nov., comb. nov.

    Science.gov (United States)

    Johansen, J E; Nielsen, P; Sjøholm, C

    1999-07-01

    Phenotypic data indicate that gliding, yellow/orange-pigmented, agar-digesting bacterial strains were members of the Cytophaga-Flavobacterium-Bacteroides (CFB) group. The strains were isolated from the surface of the marine benthic macroalga Fucus serratus L. and the surrounding seawater at three localities in Danish waters. The bacteria were Gram-negative, flexirubin-negative, aerobic, catalase-positive and oxidase-negative and were psychrophilic and halophilic. All strains utilized D-fructose, L-fucose and alpha-ketobutyric acid and degraded alginic acid, carrageenan, starch and autoclaved yeast cells. Amplification with primers specific for repetitive extragenic palindromic elements by PCR divided the strains of this study into two groups. Both groups showed unique PCR amplification patterns compared to reference strains of the CFB group. Phylogenetic analysis of 16S rDNA sequences showed association of these organisms and [Cytophaga] lytica at the genus level. Hybridization of total chromosomal DNA revealed that the new strains and [Cytophaga] lytica ATCC 23178T were clearly distinct from each other and other previously described species of the CFB group. A new genus is described, Cellulophaga gen. nov. comprising two new species, Cellulophaga baltica gen. nov., sp. nov. (NN015840T = LMG 18535T) and Cellulophaga fucicola gen. nov., sp. nov. (NN015860T = LMG 18536T), as well as the emendation of [Cytophaga] lytica to Cellulophaga lytica gen. nov., comb. nov. PMID:10425785

  5. The leader peptide of MMTV Env precursor localizes to the nucleoli in MMTV-derived T cell lymphomas and interacts with nucleolar protein B23

    International Nuclear Information System (INIS)

    We have previously described two nucleolar proteins, named p14 and p21, in MMTV-induced T cell lymphomas. These proteins were identified by a monoclonal antibody (M-66) generated from a nontumorigenic, immunogenic variant of S49 T cell lymphoma. While p14 was common to several MMTV-derived T cell lymphomas, p21 was found only in highly tumorigenic variants of S49 cells. Here we report that p14 is the leader peptide of the MMTV env precursor. The epitope recognized by M-66 contains a putative nuclear localization signal. Actinomycin D was found to induce redistribution of p14/p21 from the nucleolus to the nucleoplasm. p14 coimmunoprecipitated and colocalized with the cellular protein, B23. Association with B23 has been previously reported for other auxiliary nucleolar retroviral proteins, such as Rev (HIV) and Rex (HTLV)

  6. HIV-specific humoral and cellular immunity in rabbits vaccinated with recombinant human immunodeficiency virus-like gag-env particles

    International Nuclear Information System (INIS)

    Recombinant human immunodeficiency virus type-1 (HIV-1)-like gag-env particles produced in mammalian cells were inoculated into two New Zealand white rabbits. In parallel, two control rabbits were inoculated with the homologous HIV-1 virions inactivated by ultraviolet light (uv) and psoralen treatments. The humoral and cellular immune responses to HIV-1 were evaluated for both groups of animals. Recombinant particles elicited humoral immunity that was specific for all the viral structural proteins. The antibodies recognized both denatured and nondenatured proteins. Moreover, the sera neutralized the in vitro infectivity of the homologous virus in CEM cells. Importantly, the recombinant particles also generated a T helper response by priming with the HIV proteins. Similar results were observed with inactivated virus immunization. Therefore, the authors results suggest that the recombinant HIV-like particles elicit functional humoral immunity as well as cellular immunity and represent a novel vaccine candidate for AIDS

  7. Bases genéticas del dolor Genetic foundations of pain

    Directory of Open Access Journals (Sweden)

    P. Armero

    2004-11-01

    Full Text Available La percepción de la sensación dolorosa es un proceso complejo en el que intervienen mútiples procesos bioquímicos bien conocidos junto con otros de integración cortical desconocidos hasta el momento. La existencia de diferencias individuales en la respuesta al estímulo doloroso es una observación bien conocida que sugiere qué factores genéticos pueden estar implicados en la modulación de la respuesta a estímulos dolorosos. Existen dos aproximaciones experimentales para estudiar la implicación del genotipo en la respuesta al estímulo doloroso, los estudios de ligamiento y los estudios de asociación. Hasta el momento los estudios de ligamiento han permitido asociar mutaciones en el gen TRKA con el síndrome de insensibilidad congénita al dolor con anhidrosis (CIPA y el gen CACNL1A4 y la migraña hemipléjica familiar (FHM. Los estudios de asociación son escasos y se han centrado principalmente en el estudio de pacientes con migraña. En este trabajo revisamos los estudios llevados a cabo hasta el momento en diferentes laboratorios y planteamos nuevas perspectivas de futuro.Perception of pain is a complex process which implies multiple biochemical pathways together with unknown processes of cortical integration. The existence of individual differences in the response to painful stimuli suggests that genetic factors can be involved in its modulation. Two different experimental approaches have been developed to study the implication of genotype in the response to pain: linkage studies and the association studies. Up to now linkage studies have allowed the association of TRKA gene mutations with the syndrome of congenital insensitivity to pain with anhidrosis (CIPA and CACNL1A4 gene mutations with the familial hemiplegic migraine (FHM. Few association studies have been performed until now, and have been focused on the study of patients with migraine. Here we review the studies carried out up to now in different laboratories and suggest

  8. Diversidad genética, entre y dentro de los mayores grupos humanos

    Directory of Open Access Journals (Sweden)

    Barbujani, G.

    2003-01-01

    Full Text Available Varios estudios están de acuerdo cuando reportan que cerca del 85% de la diversidad del ADN autosomal y de los loci de las proteínas se debe a diferencias entre individuos dentro de la misma población, mientras que las diferencias entre los grupos de diferentes continentes son responsables de solamente 10% de la variación genética total. Estos resultados están en conflicto con nociones populares de razas humanas claramente distintas y relativamente homogéneas, y nos hacen cuestionar la utilidad de clasificaciones étnicas en diagnósticos médicos, en el campo forense y en genética farmacológica. Nuevos datos obtenidos de inserciones polimórficas de Alu y del cromosoma Y confirman los resultados previos, aunque indican una diversidad mayor en algunos (pero no todos los loci del cromosoma Y. Estos datos nos permiten investigar dos preguntas: (1 si las diferencias continentales, aunque pequeñas, son suficientemente grandes como para asignar a individuos a sus continentes basados en sus genotipos; (2 si los genotipos observados se agrupan en grupos de población o continentales cuando el origen de la muestra se ignora. Usando varios métodos estadísticos, veremos que los errores de clasificación son por lo menos de un 30% para los polimorfismos autosomales bi-alélicos, y de un 27% para el cromosoma Y. Cuatro series de datos genéticos de todo el mundo sugieren la existencia de grupos de genotipos diferentes, pero que éstos cuatro grupos no coinciden el uno con el otro. Adicionalmente, estudios de bloques de ADN del genoma humano indican que la mayor parte de dichos bloques es compartida entre los continentes, con solamente un pequeño porcentaje siendo específico a ciertos continentes. Estos resultados no indican que haya una base clara para subdividir a los humanos en grupos biológicamente definidos. Este puede no ser un problema en áreas aplicadas de genéticas, dado que los métodos rápidos para obtener genotipos individuales

  9. Diversidad genética en poblaciones de agaves pulqueros (Agave spp. del nororiente del Estado de México

    Directory of Open Access Journals (Sweden)

    Guillermo Alfaro Rojas

    2007-01-01

    Full Text Available Se analizaron seis poblaciones de magueyes pulqueros: "Manso" (Agave salmiana var. "Salmiana", "Ayoteco" (Agave salmiana var. "Ayoteco", "Verde", "Carrizo" (Agave mapisaga Trel, "Negro" y "Xilometl", del Nororiente del Estado de México, para determinar la diversidad genética entre y dentro de las mismas, obtener las huellas genéticas correspondientes, y hacer comparaciones para diferenciar sus variantes genéticas. Se utilizaron marcadores moleculares tipo RAPD (Polimorfismos en el ADN Amplificados al Azar y algunas variables morfológicas. El análisis de varianza mostró diferencias significativas (P ≤ 0.01 entre poblaciones para: altura de planta, número de hojas, largo de hojas, ancho de hojas, número de espinas laterales y longitud de espina principal. Los discriminantes morfológicos que permitieron separar a las poblaciones en cuatro grupos, fueron: longitud de espina principal y número de espinas laterales, mismas que explicaron 94 % de la variación. El porcentaje total de loci polimórficos fue de 73.2 % pero dentro de poblaciones hubo reducida variabilidad genética, con porcentajes de loci polimórficos que variaron de 12.2 % para el maguey "Verde" hasta 32.5 % en los magueyes "Manso" y "Ayoteco". La diversidad genética de Nei (H promedio fue de 0.28 ± 0.20, lo que confirma la baja diversidad en las poblaciones. El grado de flujo genético fue bajo (Nm = 0.24, lo que indica que hay menos de un migrante por generación entre las seis poblaciones. El dendrograma obtenido con RAPD mostró dos grandes grupos, cada uno de los cuales incluyó tres variantes relacionadas. En el primer grupo Agave mapisaga Trel ("Carrizo" se relacionó con "Verde" y con "Xilometl", con valores de identidad de 0.91 y 0.82, respectivamente. En el segundo grupo hubo una asociación muy cercana entre el maguey "Manso" (Agave salmiana var. "Salmiana" y los magueyes "Negro" y "Ayoteco" (0.89 y 0.83.

  10. Anemia Falciforme: Um Problema Nosso. Uma abordagem bioética sobre a nova genética

    Directory of Open Access Journals (Sweden)

    Diniz Debora

    2003-01-01

    Full Text Available Este artigo analisa uma das ações educativas adotadas pelo Ministério da Saúde no campo das hemoglobinopatias: o folheto informativo Anemia Falciforme: Um Problema Nosso. O objetivo é discutir as premissas e os valores morais que se encontram associados a iniciativas no campo da educação genética, tendo as políticas públicas sobre anemia falciforme no Brasil como estudo de caso. A análise mostra que o conteúdo do folheto oscila entre políticas de prevenção para doenças e promoção de direitos fundamentais, uma característica da nova genética. Além disso, o excesso de informação biomédica especializada no folheto dificulta sua divulgação em massa. Os resultados encontrados foram discutidos à luz do debate bioético contemporâneo sobre a nova genética.

  11. Relaciones genéticas en localidades de Salta, Argentina: ¿qué reflejan las medidas de distancia?

    Directory of Open Access Journals (Sweden)

    Albeza, María V.

    2010-01-01

    Full Text Available A partir de datos de once STRs autosómicos se calcularon diferentes coeficientes de distancia genética en cinco poblaciones de la provincia de Salta y se elaboraron los dendrogramas correspondientes. Cuando se incorporaron datos de la bibliografía, se amplió el análisis a 16 poblaciones (siete de la provincia y nueve de diferentes regiones del país, reduciéndose a 9 el número de loci comunes utilizados en la estimación de las diferentes medidas de distancia. Las representaciones gráficas no reflejaron las vinculaciones esperadas en función de sus proximidades geográficas, pues hubo una amplia gama de asociaciones, reflejando inconsistencias mutuas. De acuerdo al test de Mantel, no existe correlación con la matriz de distancia geográfica en cada caso (0,23 < r < 0,40 con niveles de significación que variaron entre 0,22 y 0,98, por lo que las distancias genéticas no tuvieron relación con la proximidad geográfica entre poblaciones. Tampoco existió correlación entre STRs y otros marcadores genéticos para localidades de la provincia (r = 0,10163; t = 2,3918, p = 0,9916

  12. Türkiye’de ortaöğretim okullarındaki öğrencilerin gençlik liderlik özelliklerinin incelenmesi

    OpenAIRE

    Cansoy, Ramazan

    2015-01-01

    Amaç: Bu çalışmada ortaöğretim okullarındaki öğrencilerin gençlik liderlik özelliklerinin incelenmesi amaçlanmıştır. Yöntem: Bu çalışma tarama modelindedir. Veri toplama aracı olarak Gençlik Liderlik Özelikleri Ölçeği kullanılmıştır. Araştırmaya Üsküdar ve Beykoz ilçelerinde bulunan 6 tane ortaöğretim kurumuna devam eden 1123 tane öğrenci katılmıştır. Toplanan veriler, parametrik olmayan testlerden Mann Whitney-U ve Kruskal Wallis kullanılarak analiz edilmiştir. Bulgular: Öğrencilerin, güv...

  13. ANALISIS GEN HAEMAGGLUTININ PADA VIRUS CAMPAK LIAR

    Directory of Open Access Journals (Sweden)

    Subangkit Subangkit

    2015-05-01

    Full Text Available AbstrakPenyakit Campak disebabkan oleh virus campak yang termasuk genus Morbilivirus dan Family Paramyxoviridae. Penyakit campak masih menjadi masalah kesehatan karena masih ditemukan Kejadian Luar Biasa (KLB di Indonesia. Salah satu penyebab terjadinya KLB tersebut diduga sebagaiakibat perbedaan antigenesitas antara strain vaksin yang digunakan dengan strain virus campak liar yang beredar di Indonesia. Penelitian ini bertujuan mendapatkan gambaran tentang karakteristik genetik gen Haemagglutinin virus campak liar yang ada di Indonesia. Spesimen yang digunakan sebanyak 27 isolat virus penyebab KLB dari 17 propinsi selama periode tahun 2003-2010. Isolat virus dilakukan pemeriksaan secara RT-PCR dan sekuensing dengan metode Sanger. Hasil sekuensing dianalisis dengan menggunakan perangkat lunak Bioedit 7.0 dan MEGA 4.0. Hasil penelitian didapatkan perbedaan 10 asam amino antara virus campak strain vaksin CAM-70 dan virus campak liar pada posisi D416N; K424T; V451M; N455T; V466I; I473T; F476L; Y481S atau Y481N; H495N; G505D. Kesimpulan penelitian ini adalah terdapat perbedaan karakteristik genetik antara virus campak liar di Indonesia berbeda dengan strain virus vaksin CAM-70.Kata kunci : Campak, Analisis Molekuler, Hemagglutinin, CD46AbstractMeasles is caused by virus belonging to the genus Morbilivirus and Family Paramyxoviridae. Measles is still a public health problem because outbreak of measles still found in Indonesia. Outbreak is suspected as a result of differences in antigenicity between vaccine strains used with wild-type measles virus strains circulating in Indonesia. This study aims to get genetic characteristics of wild-type measles virus haemagglutinin gene in Indonesia. The specimens were used 27 viral isolates from 17 provinces period 2003-2010. Viral isolates examined by RT-PCR and sequencing with Sanger method. Sequencing analysis were conducted using Bioedit 7.0 and MEGA 4.0 software. The results showed 10 amino acid differences

  14. Identificación de los polimorfismos G1 y G8 del gen GDF9 en ovinos criollos Araucanos

    Directory of Open Access Journals (Sweden)

    E Paz

    2014-01-01

    Full Text Available En la especie ovina se han descrito una serie de polimorfismos en genes de efecto mayor relacionados con la actividad reproductiva. Las mutaciones ubicadas en el gen de efecto mayor GDF9 se han asociado con el incremento de la tasa ovulatoria y el tamaño de la camada. GDF9 es un factor celular secretado por el ovocito y es miembro de la familia de factores de crecimiento transformante (TGF-β localizado en el cromosoma 5 ovino. El objetivo de este trabajo fue identificar la presencia de los polimorfismos en los sitios G1 y G8 en el gen GDF9 en ovinos criollos Araucanos. Se extrajo el ADN de 100 muestras sanguíneas para posteriormente determinar la presencia de las mutaciones utilizando la técnica PCR-RFLP. Fue amplificada una región de 462 pb correspondiente al exón 1, la cuál fue digerida con la enzima de restricción HhaI, el siguiente fragmento amplificado fue de 139 pb correspondiente al exón 2, siendo digerido con la enzima Ddel. Se identificó la presencia del polimorfismo G1 con una frecuencia genotípica del 0,56 (genotipo GG, 0,44 (genotipo GA y una frecuencia alélica de 0,78 para el alelo (G y 0,22 para el alelo (A. No se detectó la presencia de polimorfismo G8. Este es el primer reporte de este polimorfismo en ovinos en Chile que podría servir como un marcador genético de prolificidad para la selección de ovinos criollos Araucanos.

  15. Genetic Analysis of Relative Cell Injury Percentage and Some Yield Contributing Traits in Wheat Under Nomral and Heat Stress Conditions Análisis Genético del Porcentaje Relativo de Daño celular y algún Rasgo que Contribuye al Rendimiento en Trigo bajo Condiciones Normales y de Estrés Térmico

    Directory of Open Access Journals (Sweden)

    Jehanzeb Farooq

    2011-12-01

    Full Text Available Several wheat genotypes were screened against heat stress. Seven wheat (Triticum aestivum L. cultivars obtained after screening against heat classified as tolerant, moderately tolerant, and susceptible to heat stress, were mated in a complete diallel mating system to study the inheritance pattern of relative cell injury percentage (cell injury % and some yield contributing parameters under normal and heat stress conditions. Significant genotypic differences were found (P Varios genotipos de trigo se discriminaron para estrés térmico. De ellos siete cultivares de trigo (Triticum aestivum L. incluyendo tolerantes, moderadamente tolerantes, y susceptibles a estrés térmico obtenidos después de discriminar con calor se aparearon en un sistema de apareamiento de dialelo completo para estudiar el patrón de heredabilidad del porcentaje de daño celular relativo (daño celular % y algunos parámetros que contribuyen al rendimiento bajo condiciones normales o de estrés térmico. Se encontraron diferencias genotípicas significativas (P < 0.01 para todos los rasgos estudiados bajo ambas condiciones. Pruebas de ajuste revelaron adecuación parcial para rasgos como días a espigadura y días a madurez en ambas condiciones pero mostraron suficiencia completa para área hoja bandera y daño celular % en ambas condiciones. El modelo para producción de grano por planta y biomasa por planta fue completamente suficiente bajo condiciones normales y completamente adecuado en estrés. El componente aditivo de variación genotípica (D fue significativo para todos los rasgos estudiados y más que los componentes de dominancia H1 y H2. Los valores de la proporción de genes con efectos positivos y negativos en los progenitores (H2/4H1 demostraron distribución desigual de genes dominantes en los progenitores para casi todos los rasgos excepto área de hoja bandera, producción de grano, y daño celular % en ambas condiciones. Se encontraron estimaciones

  16. Chancen der Beteiligung privater Haushalte am Produktivvermögen

    OpenAIRE

    Thiele, Silke

    2000-01-01

    Unter den jetzigen Rahmenbedingungen halten im Durchschnitt lediglich 12 % der Haushalte der alten bzw. 3 % der Haushalte der neuen Bundesländer Aktien, d.h. dieser Anteil an Haushalten beteiligt sich bereits freiwillig am Produktivvermögen. Um Beteiligungen in größerem Umfang zu realisieren, müßten folglich hohe Anreize geschaffen werden. Die geringsten Präferenzen Aktien zu bilden, haben Haushalte mit geringem Einkommen, geringer Vermögensstreuung und geringem Vermögen. Die Präferenzen dies...

  17. History of the Juliusruh ionospheric observatory on Rügen

    Science.gov (United States)

    Weiß, J.

    2016-02-01

    The history of the Juliusruh ionospheric observatory on Rügen is closely connected to the history of ground-based ionospheric sounding. After a short introduction to the ionospheric research and the sounding technique, the founding of the Juliusruh station in 1954 and its development until today are described. The different methods of ground-based sounding - as far as they apply to Juliusruh - are briefly discussed. The condition of life and work in a small team on the island of Rügen, remote from the respective parent institute, is also the subject of this article, whose author headed Juliusruh Station from 1965 to 2004.

  18. Craniostenose em gêmeos: estudo genético

    Directory of Open Access Journals (Sweden)

    Walter Carlos Pereira

    1968-09-01

    Full Text Available É relatada a ocorrência de formas clínicas diversas de craniostenose em gêmeos de sexo diferente. A menina apresentava obliteração completa da sutura coronaria e dos dois terços anteriores da sutura sagital; no menino a sutura sagital era a única afetada. O estudo genético mostrou que a craniostenose independe de aberrações cromossômicas, indicando ser transmitida por gens recessivos raros de natureza autossômica.

  19. Algoritmos genéticos para planejamento em inteligencia artificial

    OpenAIRE

    Lecheta, Edson Martins

    2011-01-01

    Este trabalho apresenta uma revisão bibliográfica atualizada sobre duas grandes áreas da Inteligência Artificial: Planejamento e Algoritmos Genéticos. A pesquisa se estende pela criação de modelos genéticos implementados em um sistema planejador dedicado à resolução de uma conhecida classe de problemas de planejamento, usando bibliotecas de código de domínio público em ambas as áreas. Uma análise dos resultados motivou a remodelagem e nova implementação, alterando a plataforma e o sistema ope...

  20. A Virtual Reality Framework to Optimize Design, Operation and Refueling of GEN-IV Reactors

    International Nuclear Information System (INIS)

    Many GEN-IV candidate designs are currently under investigation. Technical issues related to material, safety and economics are being addressed at research laboratories, industry and in academia. After safety, economic feasibility is likely to be the most important criterion in the success of GEN-IV design(s). Lessons learned from the designers and operators of GEN-II (and GEN-III) reactors must play a vital role in achieving both safety and economic feasibility goals

  1. A Virtual Reality Framework to Optimize Design, Operation and Refueling of GEN-IV Reactors.

    Energy Technology Data Exchange (ETDEWEB)

    Rizwan-uddin; Nick Karancevic; Stefano Markidis; Joel Dixon; Cheng Luo; Jared Reynolds

    2008-04-23

    many GEN-IV candidate designs are currently under investigation. Technical issues related to material, safety and economics are being addressed at research laboratories, industry and in academia. After safety, economic feasibility is likely to be the most important crterion in the success of GEN-IV design(s). Lessons learned from the designers and operators of GEN-II (and GEN-III) reactors must play a vital role in achieving both safety and economic feasibility goals.

  2. Analisis Kata Penghubung He (和), Gen ( 跟) Dan Yu (与) Dalam Kalimat Bahasa Mandarin

    OpenAIRE

    Tarigan, Cicilia Aprilina Kartika

    2013-01-01

    The tittle of this paper is “Analisis Kata Penghubung He, Gen, dan Yu dalam Kalimat Bahasa Mandarin. Researcher analyzes the use of Chinese cunjuctions, he, gen, yu in Chinese sentences. Actually he, gen and yu have the same meaning but differently in their own characteristics. The concepts of the thesis are The methodology on the thesis is descriptive method. The author analyze about characteristic and similarities and differences of cunjuctions, hen, gen, and yu. By this, author is try to ...

  3. Estimación de parámetros genéticos para características de crecimiento en borregos Katahdin usando diferentes modelos

    Directory of Open Access Journals (Sweden)

    Coralia Inés V. Manzanilla Pech

    2012-01-01

    Full Text Available Se estimaron parámetros genéticos para características de crecimiento en corderos Katahdin, usando seis variantes del modelo animal. Se usó información de pesos al nacimiento (BW; n= 13,099, al destete ajustado a 75 d (WW; n= 11,509 y posdestete ajustado a 120 d (AW; n= 6,886 tomada durante 7 años (2004-2010 en 20 estados de la República Mexicana. Los análisis se hicieron ignorando o incluyendo efectos maternos. El modelo más sencillo incluyó el efecto genético aditivo directo como el único efecto aleatorio. El modelo más completo incluyó los efectos genéticos directo y materno, la covarianza entre ellos, y el efecto del ambiente permanente materno. Para seleccionar el mejor modelo se usó la prueba de razón de verosimilitud. Cuando los efectos maternos no fueron incluidos en el modelo, los estimadores de la heredabilidad directa y de la varianza genética directa resultaron sobreestimados. Las heredabilidades directas con el mejor modelo fueron 0.18 ± 0.03, 0.30 ± 0.04 y 0.20 ± 0.05 para BW, WW y AW, respectivamente. Las heredabilidades maternas también variaron dependiendo del modelo, de 0.05 a 0.23, 0.00 a 0.12, y 0.09 a 0.25 para BW, WW y AW. El ignorar los efectos maternos en el modelo resultaría en una evaluación genética equivocada para las características de crecimiento en borregos Katahdin.

  4. Ambiente genético del Gen blaCTX-M-12 en aislamientos hospitalarios de Klebsiella pneumoniae

    Directory of Open Access Journals (Sweden)

    Yamile Adriana Celis Bustos

    2009-06-01

    Full Text Available The blaCTX-M-12 gene’s genetic environmnt in Klebsiella pneumoniae hospital isolates Resumen: En Colombia se han detectado genes del grupo CTX-M-1 con alta frecuencia en aislamientos de Klebsiella pneumoniae causantes de infección intrahospitalaria. El conocimiento de los factores genéticos que pueden favorecer la diseminación de estos genes entre especies bacterianas es un aspecto importante para el control de la resistencia. En este estudio se identificaron los plásmidos portadores del gen blaCTX-M-12 en 21 aislamientos clínicos de K. pneumoniae. Se evaluó por conjugación la transferencia de resistencia a antibióticos. Integrones, secuencias de inserción y otros elementos genéticos fueron detectados por amplificación del ADN plasmídico con la reacción en cadena de la polimerasa (PCR. Mediante análisis por PCR se determinó la relación entre el gen blaCTX-M-12 y los elementos genéticos detectados. En todos los aislamientos, el gen blaCTX-M-12 se encontró en plásmidos conjugativos de tamaños entre 65 y 106 kpb. La transferencia por conjugación de estos elementos móviles puede explicar la amplia diseminación de este gen entre enterobacterias causantes de infección nosocomial en hospitales de Bogotá, Colombia. El gen blaCTX-M-12 se encontró corriente abajo de ISEcp1, secuencia de inserción que se ha asociado con la movilización de determinantes genéticos de resistencia. Los promotores de ISEcp1, detectados por análisis de secuencia, pueden facilitar la expresión de la cefotaximasa codificada por este gen.Palabras clave: resistencia a antibióticos; elementos genéticos móviles; gen blaCTX-M-12; plásmidos conjugativos;  Klebsiella pneumoniae. Abstract: Genes from CTX-M-1 group have been detected with great frequency in Colombia in intrahospital infection-causing Klebsiella pneumoniae isolates. Knowledge regarding the genetic factors favouring such genes’ dissemination amongst bacterial species is an

  5. Estructura genética del sistema de asimilación de nitrato y regulación global de la asimilación de nitrógeno en la cianobacteria synechococcus sp.

    OpenAIRE

    Luque Romero, Ignacio

    1994-01-01

    Los objetivos de este trabajo han sido profundizar en el estudio de la estructura genética de los genes implicados en la asimilación de nitrato en Synechococcus sp. PCC 7942, y contribuir al esclarecimiento del mecanismo de regulación de dichos genes mediante la proteína NtcA.

  6. Diversidad genética de aislamientos de Phytophthora infestans en plantaciones de papa en Costa Rica con el uso de RAPDs

    OpenAIRE

    Oswaldo P\\u00E1ez; Roberto Valverde; Luis G\\u00F3mez; Arturo Brenes

    2005-01-01

    Para determinar la diversidad genética de Phytophthora infestans en Costa Rica, 62 aislamientos de este patógeno fueron recolectados en plantaciones de papa en las zonas de Cartago, Zarcero, Fraijanes y Heredia durante 1999-2001 y analizados con el uso de RAPDs. Todos los aislamientos fueron previamente evaluados para el tipo de apareamiento y la resistencia al metalaxyl. Se seleccionó 11 imprimadores con los cuales fue posible formar 17 grupos RAPD cuyas frecuen...

  7. Reação de genótipos de soja ao alumínio em hidroponia e no solo

    Directory of Open Access Journals (Sweden)

    Souza Luiz Augusto Copati

    2001-01-01

    Full Text Available O objetivo deste trabalho foi avaliar os genótipos de soja BR86-5974, BR86-7396, Dourados, Doko RC, EMGOPA 305, IAC-9, BR-9 (Savana, UFV-1, UFV-9 e UFV Araguaia em relação à tolerância ao alumínio (Al em hidroponia e em solo. Na solução com Al foi medido o comprimento radicular. Em solo com 49% de saturação de Al avaliou-se área foliar, altura de planta, altura de inserção da primeira vagem, produção de matéria seca, produção de grãos e índice de colheita. Os genótipos BR86-7396 e IAC-9 são os de maior tolerância ao Al, e UFV-1 mostrou o pior desempenho. Houve correlação significativa entre alongamento radicular e produção de grãos (r = 0,705, área foliar (r = 0,645 e produção de matéria seca (r = 0,634. Isto indica que experimentos em hidroponia e solo são igualmente eficientes na seleção de soja tolerante ao alumínio. A variabilidade detectada sugere que o conjunto de genótipos de soja possui ampla variabilidade genética, o que é desejável em programas de melhoramento com o objetivo de elevar estabilidade de produção no Cerrado.

  8. Consecuencias genéticas de igualar las contribuciones familiares en programas de conservación

    OpenAIRE

    Sánchez Molano, Enrique

    2010-01-01

    En poblaciones en riesgo de extinción, puede ser necesario establecer un programa de conservación ex situ que, desde el punto de vista genético, permita controlar su estructura reproductiva para minimizar los efectos de la consanguinidad y la deriva. En tales programas, a menudo se recomienda igualar las contribuciones familiares al grupo reproductor (estrategia CI) para maximizar el censo efectivo. Este procedimiento causa una relajación parcial de la selección natural, impidiendo su acción ...

  9. Mejoramiento genético para tolerancia a altas temperaturas y resistencia a mosaico dorado en frijol común

    OpenAIRE

    Juan Carlos Rosas; Aracely Castro; James S. Beaver; Carlos A. P\\u00E9rez; Adri\\u00E1n Morales; Rogelio Lepiz

    2000-01-01

    Mejoramiento genético para tolerancia a altas temperaturas y resistencia a mosaico dorado en frijol común. Entre 1994-95 se identificaron fuentes de frijol tolerantes a altas temperaturas mediante la evaluación de germoplasma y líneas mejoradas en Choluteca y Nacaome (≤ 50 msnm), en la región Sur de Honduras. La tolerancia al calor de los mejores genotipos fue confirmada en Geneva, Nueva York, EE.UU., bajo condiciones de invernadero con temperatura controlada (35...

  10. Organización de los nucleosomas y regulación genómica en Schizosaccharomyces pombe

    OpenAIRE

    Serrano Pérez, Rebeca

    2014-01-01

    [ES]El genoma de los organismos eucariotas está organizado en cromatina, estructura cuya unidad fundamental es el nucleosoma. Éstos, facilitan el empaquetamiento del DNA dentro del núcleo y son esenciales en su mantenimiento, permitiendo la regulación de procesos genómicos básicos para la función celular. El trabajo presentado en esta tesis doctoral pretende contribuir al conocimiento de la organización de los nucleosomas y su papel en procesos relacionados con la regulación del genoma en ...

  11. Tipificación de marcadores genéticos sanguíneos en raza Hereford

    OpenAIRE

    Quinteros, Indalecio Rodolfo; Tejedor, Eugenio Daniel; Poli, Mario Andrés; Antonini de Ruiz, Alicia Graciela

    1981-01-01

    El paso inicial de esta investigación ha sido tipificar al Bovino Hereford de Argentina para definirlo mediante la metodología de la Inmunogenética. Se buscaron "expresiones" propias y coincidencias con los "marcadores genéticos sanguíneos" descubiertos en esta raza por otros países. Su gran adaptabilidad a "hábitats" diferentes induce a mantener intacto su germoplasma y enriquecerlo con el agregado de nuevos genes. No obstante su homogeneidad racial, el Hereford Argentino presenta destacado ...

  12. Correlaciones fenotípicas, genéticas y ambientales en cucurbita moschata duch. ex poir

    OpenAIRE

    Baena García D.; Espitia Camacho M.; Vallejo Cabrera F. A.

    2006-01-01

    El estudio estimó las correlaciones fenotípicas, genéticas y ambientales entre doce caracteres agronómicos en dos dialélicos de zapallo (uno entre cinco variedades y otro entre cinco líneas S1, originadas de las anteriores). Se usó un diseño en bloques completos al azar con 15 tratamientos (5 progenitores + 10 cruzamientos F1) para cada dialélico y cinco repeticiones. Los resultados señalaron mayor estimación de las correlaciones (en magnitud y significancia estadística) a favor del dialélico...

  13. Genética molecular y biogerontología en la era postgenómica

    OpenAIRE

    Michán Aguirre Shaday

    2011-01-01

    RESUMEN La forma de estudiar la genética ha progresado notablemente en las últimas decadas. Sus orígenes se remontan al estudio de los caracteres hereditarios, seguido por el descubrimiento de los genes y los cromosomas hasta conocer la estructura del ADN. Este último evento impulsó el desarrollo de la tecnología del ADN recombinante y de la secuenciación masiva y automatizada, los cuales permitieron posteriormente determinar la anatomía de los genomas. Todos estos descubrimientos ...

  14. Genética y Conducta en el Síndrome de Déficit Atencional e Hiperactividad:

    OpenAIRE

    Francisco Aboitiz; Carolina Schroter

    2006-01-01

    El síndrome de déficit atencional e hiperactividad es una condición de alta prevalencia entre niños y adultos, que puede afectar seriamente el desempeño escolar y laboral. En este artículo revisamos los aspectos genéticos de este síndrome y los interpretamos en función de una falla en el sistema de señalización dopaminérgica, que se caracteriza por un bajo coeficiente señal/ruido y una excesiva recaptación presináptica del neurotransmisor liberado.

  15. GA-Ensemble : optimización de conjuntos de clasificadores mediante algoritmos genéticos

    OpenAIRE

    Ordóñez Morales, Francisco Javier

    2007-01-01

    El objetivo general de este Proyecto Fin de Carrera es desarrollar un algoritmo optimizado de generación de conjuntos de clasificadores heterogéneos basándose en el algoritmo GA-Stacking. Los objetivos específicos son: - Determinar los métodos de generación de clasificadores más adecuados al proceso de creación del conjunto de clasificadores. - Definir los parámetros adecuados de los algoritmos genéticos a utilizar para la generación del conjunto de clasificadores, haciendo ...

  16. Diversidade genética entre progênies e matrizes de rambutan

    OpenAIRE

    Renata Aparecida de Andrade; Ester Wickert; Antonio Baldo Geraldo Martins; Eliana Gertrudes de Macedo Lemos

    2012-01-01

    O rambutan é uma frutífera exótica que apresenta alto potencial de mercado, e suas mudas podem ser obtidas por sementes ou vegetativamente. A produção de mudas via sementes é rotineiramente feita no Estado de São Paulo, tendo-se alta variabilidade no pomar, além de demorar mais tempo para entrar em produção. Embora caracteres morfológicos sejam amplamente usados na diferenciação de variedades, as técnicas moleculares permitem a comparação e a identificação genética dos materiais. Diante disso...

  17. Bases Genéticas de la Ataxia Espinocerebelosa tipo 2

    Directory of Open Access Journals (Sweden)

    Luis E. Almaguer Mederos

    2008-01-01

    Full Text Available Aborda la genética clásica y molecular de la ataxia espinocerebelosa tipo 2, enfermedad neuro degenerativa que alcanza las mayores tasas de prevalencia e incidencia del mundo en Holguín, y que constituye un serio problema de salud. Desde el descubrimiento de la mutación causal de la enfermedad en 1996, se han obtenido avances significativos en la comprensión de los mecanismos moleculares involucrados en el proceso patológico, y en sus implicaciones clínicas. Tales hallazgos han permitido el desarrollo de modelos celulares y animales de la enfermedad, que constituyen herramientas indispensables para la búsqueda de alternativas terapéuticas orientadas al tratamiento de las personas afectadas.

  18. Disseny i implementació d'un robot 3R genèric

    OpenAIRE

    Ballbè Chaler, Marc

    2015-01-01

    Aquest projecte s'ha realitzat al laboratori del Institut de Robòtica i Informàtica Industrial ubicat en la Facultat de Matemàtiques i Estadística de la Universitat Politècnica de Catalunya. Els objectius d'aquest projecte han sigut diversos, encara que tots s'engloben en l'anàlisi genèrica d'un robot amb tres graus de llibertat de rotació i la implementació d'un prototip. Un dels objectius principals ha sigut la resolució de la cinemàtica directa i inversa d'un robot amb...

  19. Divergência genética em genótipos de girassol Genetic divergence in sunflower genotypes

    Directory of Open Access Journals (Sweden)

    Edson Perito Amorim

    2007-12-01

    Full Text Available Uma investigação sobre a diversidade genética entre 15 genótipos de girassol, por meio de 12 características agronômicas, foi implementada no Instituto Agronômico, Campinas, Brasil. Análises de variância univariada e multivariada revelaram diferenças entre os genótipos. A distância generalizada de Mahalanobis indicou um alto grau de divergência genética. Os genótipos foram agrupados em três grupos. As características início do florescimento, 50% do florescimento, número de folhas e altura da inserção do capítulo contribuíram com grande parte da divergência genética observada. Por meio desses resultados, é possível identificar materiais divergentes e com características agronômicas complementares para o desenvolvimento de novos cultivares superiores.An investigation about the genetical diversity among fifteen sunflower genotypes using twelve agronomical characteristics was implanted at the Agronomic Institute, Campinas Brazil. Univariate and multivariate analyses of variance revealed the presence of differences among the genotypes. The generalized distance of Mahalanobis indicated the presence of genetic diversity. The genotypes were grouped into tree clusters. Among the investigated characteristics, the beginning of flowering, 50% flowering, leaf number and head height of chapter insertion exhibited high contribution towards genetic divergence. Through these studies it is possible to identify divergent material with further agronomical features for the development of new superior sunflower cultivars.

  20. Linfohistiocitosis hemofagocítica, el espectro desde la enfermedad genética al síndrome de activación macrofágica Hemophagocityc Lymphohistiocytosis: A Spectrum from the Genetic Disorder to the Macrophage Activation Syndrome

    Directory of Open Access Journals (Sweden)

    Oscar Porras

    2011-06-01

    Full Text Available El compromiso de la regulación de la inflamación produce activación excesiva y expansión de macrófagos y linfocitos T que desencadenan una reacción inflamatoria severa, sin vías naturales de control. Los trastornos hemofagocíticos son la traducción clínica de este proceso inflamatorio. La linfohistiocitosis hemofagocítica se refiere a todas las variantes de esta patología, y el síndrome de activación macrofágica, a la variante asociada con enfermedad autoinmune. Los casos primarios se asocian con la forma familiar autosómica recesiva y los secundarios con inmunodeficiencias primarias, infección, malignidad y enfermedades autoinmunes. El principal distintivo de este grupo de patologías es la proliferación agresiva de macrófagos e histiocitos que fagocitan otras células sanguíneas. La reducción en la actividad de las células NK produce un aumento en la activación y expansión de linfocitos T, los cuales producen grandes cantidades de citoquinas. Las citoquinas inducen activación de macrófagos y células dendríticas, infiltración tisular y producción de interleuquinas, lo que genera una reacción inflamatoria severa, responsable del daño tisular y de las manifestaciones clínicas. El curso clínico se caracteriza principalmente por fiebre prolongada, hepatoesplenomegalia y citopenias. Los estudios de laboratorio muestran aumento de ferritina, triglicéridos e hipofibrinogenemia. La hemofagocitosis en médula ósea está presente en más del 80% de los casos al diagnóstico. El tratamiento está dirigido contra el linfocito T y los histiocitos hiperactivados, combinando quimioterapia con inmunosupresores y, en algunos casos, trasplante de células madre hematopoyéticas. Este tratamiento ha producido un cambio en la sobrevida de los pacientes. El protocolo de tratamiento HLH-2004 es una guía que estandariza el tratamiento, combinando etopósido, dexametazona y ciclosporina A. En Costa Rica se han reportado 60 casos en

  1. Los usuarios ante los alimentos genéticamente modificados y su información en el etiquetado

    Directory of Open Access Journals (Sweden)

    Miren Itxaso Sebastian-Ponce

    2014-02-01

    Full Text Available OBJETIVO : Analizar la opinión que los usuarios tienen sobre alimentos genéticamente modificados y su información en el etiquetado. MÉTODOS : Realizada revisión sistemática de la literatura científica sobre los alimentos transgénicos y el etiquetado a partir de la consulta de las bases de datos bibliográficas: Medline (vía PubMed, EMBASE, ISI-Web of Knowledge, Cochrane Library Plus, FSTA, LILACS, CINAHL y AGRICOLA. Los descriptores seleccionados fueron: «organisms, genetically modified » y «food labeling». La búsqueda se realizó desde la primera fecha disponible hasta junio de 2012, seleccionando los artículos pertinentes escritos en inglés, portugués y castellano. RESULTADOS : Se seleccionaron 40 artículos. En todos ellos, se debía haber realizado una intervención poblacional enfocada al conocimiento de los consumidores sobre los alimentos genéticamente modificados y su necesidad, o no, de incluir información en el etiquetado. El consumidor expresa su preferencia por el producto no-genéticamente modificado, y apunta que está dispuesto a pagar algo más por él, pero, en definitiva compra el artículo que está a mejor precio en un mercado que acoge las nuevas tecnologías. En 18 artículos la población se mostraba favorable a su etiquetado obligatorio y seis al etiquetado voluntario; siete trabajos demostraban el poco conocimiento de la población sobre los transgénicos y, en tres, la población subestimó la cantidad que consumía. En todo caso, se observó la influencia del precio del producto genéticamente modificado. CONCLUSIONES : La etiqueta debe ser homogénea y aclarar el grado de tolerancia en humanos de alimentos genéticamente modificados en comparación con los no modificados. Asimismo, debe dejar claro su composición, o no, de alimento genéticamente modificado y la forma de producción de estos artículos de consumo. La etiqueta también debe ir acompañada de un sello de certificación de una agencia

  2. El uso de alteraciones genéticas en la estratificación por riesgo del mieloma múltiple

    Directory of Open Access Journals (Sweden)

    Esteban Braggio

    2013-08-01

    Full Text Available Los estudios genéticos han alcanzado un papel central en el estudio del mieloma múltiple (MM, al convertirse en un componente crítico en la estratificación basada en el riesgo de la enfermedad. Se han hecho grandes esfuerzos para identificar cambios genéticos que puedan predecir el resultado clínico e incluirlos en la práctica clínica diaria. La hibridización in situ fluorescente (FISH es todavía la técnica genética más utilizada en la práctica clínica, mayormente debido a su sencilla implementación y su simplicidad para el análisis de datos. El advenimiento de la genómica (hibridización genómica comparativa, secuenciación exónica o genómica completa y del transcriptoma de alta resolución (perfiles de expresión de genes - GEP y secuenciación de ARNm proveen un análisis exhaustivo de los ya definidos factores pronósticos genéticos y son herramientas útiles para la identificación de potenciales nuevos marcadores pronósticos de enfermedad en el clon tumoral de MM. Más aún, GEP ha sido exitosamente implementado en MM como una herramienta de estratificación de riesgo, siendo la de mayor poder de discriminación de resultados. De todas maneras, algunos aspectos técnicos y logísticos complejos (necesidad de una elevada purificación del clon tumoral, costo de los ensayos y complejidad en los análisis de los datos deben ser considerados antes de la incorporación definitiva de estas tecnologías de alto rendimiento dentro de los ensayos clínicos de rutina. Hasta entonces, FISH continúa siendo la herramienta estándar para la detección de anormalidades genéticas y de valoración pronóstico de enfermedad.

  3. Caracterização físico-química de frutos de genótipos de aceroleira (Malpighia emarginata D.C.

    Directory of Open Access Journals (Sweden)

    Maria Inês Sucupira Maciel

    2010-12-01

    Full Text Available No Brasil, a aceroleira, decorrente principalmente da propagação por sementes, tem dado origem a plantios comerciais cujos frutos apresentam parâmetros de qualidade diferenciados. Características físico-químicas de frutos de 18 genótipos de aceroleira (Malpighia emarginata DC. do Banco Ativo de Germoplasma da Universidade Federal Rural de Pernambuco - UFRPE foram avaliados. O rendimento em polpa variou de 41,06% (PL 40 a 72,54% (PL 43 e, com exceção do genótipo PL 37, os demais apresentaram frutos com teores de ácido ascórbico superiores a 1000 mg.100 g-1. O genótipo PL 39 destacou-se por apresentar o maior teor de ácido ascórbico (1667 mg.100 g-1, SST e flavonóis (15,04 mg.100 g-1, além de elevado teor de antocianinas, sendo, portanto, o mais promissor. Os frutos do genótipo PL 34 revelaram o maior valor de SST/ATT, indicando ser o mais doce

  4. Meet Mr. and Mrs. Gen X: A New Parent Generation

    Science.gov (United States)

    Howe, Neil

    2010-01-01

    Slowly but surely, Generation Xers have been taking over from Baby Boomers as the majority of parents in elementary and secondary education. In the early 1990s, Gen Xers began joining parent-teacher associations in the nation's elementary schools. Around 2005, they became the majority of middle school parents. By the fall of 2008, they took over…

  5. PowerGen plc report and accounts 1994

    International Nuclear Information System (INIS)

    The annual report and accounts of PowerGen plc for the year 1994 are presented. Financial highlights are quoted, followed by the Chairman's statement, reviews by the Chief Executive and Financial Directors, reports by the Auditors and Directors, balance sheets and details of the consolidated profit and loss account and principal accounting policies. A four year summary and shareholder information are included. (UK)

  6. GenBank blastx search result: AK058467 [KOME

    Lifescience Database Archive (English)

    Full Text Available ome 1p34.2-36.11 Contains the 3' end of the KPNA6 gene for karyopherin alpha 6 (importin alpha 7), a novel g...ene (DKFZp451J0118), a novel gene (MGC1203) a novel gene, a novel gene (FLJ10547), a novel gene, a novel gen

  7. GenBank blastn search result: AK288295 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK288295 J090019J01 AM157075.1 AM157075 Echinolittorina reticulata mitochondrial partial COI gen ... for cytochrome oxidase subunit I, specimen voucher Philippines ... C.2, NRET.GUI.2. INV 3e-18 1 -1 ...

  8. El desarrollo de la investigación en genética en Colombia

    Directory of Open Access Journals (Sweden)

    Carolina Isaza

    2008-06-01

    Full Text Available Durante la última década la genética y la biología molecular en Colombia han dado un gran salto en su desarrollo. Al revisar las convocatorias nacionales de salud que realiza anualmente el Ministerio de Protección Social a través de Colciencias es posible visualizarlo. Al dar una breve mirada a las cifras estadísticas desde el año 2000, encontramos que el país muestra un claro aumento de la capacidad científica y el recurso humano para investigación en salud, reflejado en el incremento del número de proyectos presentados en cada convocatoria y el aumento considerable en la inversión del país para financiar investigación en salud, llegando en el año 2008 a la cifra de 28,000 millones de pesos.En el año 2000 se presentaron para evaluación a Colciencias 133 proyectos y se aprobaron para ejecución solamente 43 por un monto de 1,585 millones de pesos, mientras que en el 2007 se presentaron 690 y aprobados 103 por un monto de 21,223 millones de pesos. Este año se han presentado 711 proyectos que se encuentran actualmente en evaluación y se dispone de 28,000 millones para financiación. Estas cifras muestran un incremento de siete veces en el número de propuestas y un crecimiento de cerca de 27 veces en el presupuesto durante los últimos 7 años. Es muy claro que los proyectos seleccionados son cada vez de mayor impacto para la salud de los colombianos y ahora entre 20% y 30% de las propuestas presentadas en la modalidad nacional, incluyen temas relacionados con genética o utilizan herramientas de biología molecular. Sin embargo, este porcentaje es mucho menor en la convocatoria regional, lo que muestra una concentración de científicos con preparación en genética y biología molecular en las principales ciuda-des del Colombia, que es lo que se ha denominado el triángulo de oro de la ciencia en Colombia (Medellín, Bogotá y Cali. Es interesante ver los desarrollos de la genética molecular para el estudio de las

  9. Caracterización genética y funcional del gen causante de la Epilepsia Lateral Temporal Autosómica Dominante

    OpenAIRE

    Morante Redolat, José Manuel

    2006-01-01

    En el primer capítulo se presenta el trabajo de identificación del gen causante de la Epilepsia Lateral Temporal Autosómica Dominante (ADLTE) mediante un rastreo de genes candidatos en familias afectadas. Una vez identificado dicho gen se procedió a diseñar las estrategias necesarias para su caracterización según la información disponible sobre el gen en aquel momento. El segundo capítulo se centra en los resultados obtenidos sobre la naturaleza de la proteína codificada por el gen iden...

  10. Propuesta de consulta para desregulación de alimentos derivados de cultivos genéticamente mejorados, desarrollados en Costa Rica Request for consultation for deregulation of foods derived from genetically enhanced crops developed in Costa Rica

    OpenAIRE

    M-Eugenia Villalobos Hernández; Ana M Espinoza Esquivel

    2009-01-01

    Objetivo: Proponer un proceso de desregulación de alimentos derivados de cultivos genéticamente mejorados desarrollados en Costa Rica, plantea un trámite a la Consulta de desregulación de un nuevo alimento desarrollado por ingeniería genética, labor que le competerá al Ministerio de Salud principalmente. Material y Métodos: La propuesta incluye procedimientos de iniciar la Consulta, conformación del equipo de evaluadores, información que se solicita en el dossier sobre alimentos derivados de ...

  11. Caracterización molecular, genética poblacional y relación biológica del nemertino Malacobdella arrokeana endocomensal de la almeja gigante Panopea abbreviata

    OpenAIRE

    Alfaya, José E. F.

    2014-01-01

    [ES] Malacobdella arrokeana Ivanov et al., 2002 es un nemertino que vive en la cavidad del manto de la almeja gigante Panopea abbreviata, ambas especies endemicas del Mar Argentino. Mediante la secuenciación y análisis del gen mitocondrial citocromo oxidasa I (COI), se corroboró que M arrokeana diverge genéticamente de M. grossa en un 11,6%, y 10,4% con respecto a M. japonica. Tanto M. arrokeana como P. abbreviata comparten el mismo nivel trófico y los mismos ítems alimentarios, compuestos en...

  12. La Enseñanza de la genética: Una propuesta didáctica para la educación secundaria obligatoria desde una perspectiva constructivista

    OpenAIRE

    Iñiguez Porras, Francisco Javier

    2006-01-01

    [spa] Frente al modelo tradicional de la enseñanza-aprendizaje de la genética, basado en la transmisión por parte del profesorado de conocimientos ya elaborados, y que no tiene en cuenta las concepciones del alumnado, nosotros hemos propuesta una secuencia didáctica basada en los principios del constructivismo. Dicha secuencia tiene en consideración las ideas de los estudiantes, tiene una cuenta las propuestas de los investigadores en didáctica de la genética y consta de diferentes actividade...

  13. Desarrollo de un sistema de expresión condicional del gen RHBDD2 para su sobreexpresión en un ratón transgénico

    OpenAIRE

    Lacunza, Ezequiel; Abba, Martin Carlos; Aldaz, C. Marcelo

    2011-01-01

    En estudios previos hemos identificado al gen RHBDD2 sobreexpresado en carcinomas invasores de mama. Hasta el momento se desconoce el rol funcional de dicha alteración aunque existen evidencias de que podría determinar un fenotipo favorable para las células tumorales. A fin de analizar in vivo las consecuencias biológicas de la sobreexpresión del gen RHBDD2, se llevó a cabo la construcción de un sistema de expresión condicional, para su inyección en células madres emb...

  14. Evaluación genética del recurso animal de los sistemas de producción de bovinos en doble propósito en Colombia / Genetic evaluation of dual purpose production systems in Colombia

    OpenAIRE

    Galeano Rivera, Adriana Patricia

    2010-01-01

    Con el objetivo de determinar el verdadero potencial genético del recurso animal disponible en los sistemas de producción bovina de doble propósito del trópico bajo colombiano, se evaluaron los registros de producción de leche por lactancia (Kg), peso al destete (Kg), intervalo entre partos (d) e Índice de Vaca, de 1.687 hembras reproductoras, durante los años 1998 y 2007. Se empleó un modelo animal mixto que incluyó los efectos genéticos aleatorios del animal, el medio ambiente permanente y ...

  15. Flexibilität organisieren: Callcenter als kundenorientierte Organisationen

    OpenAIRE

    Holtgrewe, Ursula; Kerst, Christian

    2003-01-01

    "Das Aufkommen und Wachstum der Callcenter wird häufig entlang der Frage diskutiert, ob sich damit der Taylorismus in neua rtiger und durchschlagender Weise der Dienstleistungsarbeit bemächtigt. Vor allem der Einsatz tayloristischer Kontrolltechnologien (Fernie/Metcalf 1998) und der Einsatz niedrig qualifizierter Arbeitskräfte, vorwiegend mit befristeten Verträgen oder geringfügig beschäftigt, lässt Callcenter als die Vorreiter atypischer Beschäftigung erscheinen (vgl. Flecker ...

  16. Logos versus Aisthesis. Die kunsthistorische Diaprojektion als codierendes Instrument

    OpenAIRE

    Hiller-Norouzi, Julica

    2009-01-01

    Seit die akademische Kunstgeschichte am Ende des 19. Jahrhunderts die Diaprojektion als bevorzugtes Lehrmedium für sich bestimmte, änderte sich die kunsthistorische Lehre maßgeblich. Fotografische Abbildungen von Kunstwerken prägen seitdem die visuelle Wahrnehmung von Kunst und geben eine bestimmte Sehrichtung der Wissenschaft vor. Einhergehend damit veränderte sich auch die Wahrnehmung der Medialität von Kunstwerken. Ich lote aus, welchen Einfluss die Diaprojektion mit ihren zentralperspekti...

  17. Viral RNA levels and env variants in semen and tissues of mature male rhesus macaques infected with SIV by penile inoculation.

    Directory of Open Access Journals (Sweden)

    Francis Fieni

    Full Text Available HIV is shed in semen but the anatomic site of virus entry into the genital secretions is unknown. We determined viral RNA (vRNA levels and the envelope gene sequence in the SIVmac 251 viral populations in the genital tract and semen of 5 adult male rhesus monkeys (Macaca mulatta that were infected after experimental penile SIV infection. Paired blood and semen samples were collected from 1-9 weeks after infection and the monkeys were necropsied eleven weeks after infection. The axillary lymph nodes, testes, epididymis, prostate, and seminal vesicles were collected and vRNA levels and single-genome analysis of the SIVmac251 env variants was performed. At the time of semen collection, blood vRNA levels were between 3.09 and 7.85 log10 vRNA copies/ml plasma. SIV RNA was found in the axillary lymph nodes of all five monkeys and in 3 of 5 monkeys, all tissues examined were vRNA positive. In these 3 monkeys, vRNA levels (log10 SIVgag copies/ug of total tissue RNA in the axillary lymph node (6.48 ± 0.50 were significantly higher than in the genital tract tissues: testis (3.67 ± 2.16; p<0.05, epididymis (3.08 ± 1.19; p<0.0001, prostate (3.36 ± 1.30; p<0.01, and seminal vesicle (2.67 ± 1.50; p<0.0001. Comparison of the SIVmac251 env viral populations in blood plasma, systemic lymph node, and genital tract tissues was performed in two of the macaques. Visual inspection of the Neighbor-Joining phylograms revealed that in both animals, all the sequences were generally distributed evenly among all tissue compartments. Importantly, viral populations in the genital tissues were not distinct from those in the systemic tissues. Our findings demonstrate striking similarity in the viral populations in the blood and male genital tract tissues within 3 months of penile SIV transmission.

  18. Estimación mediante RAPD's de la diversidad genética en Guadua en el departamento del Cauca, Colombia

    Directory of Open Access Journals (Sweden)

    Palacio M. Juan Diego

    2006-06-01

    Full Text Available

    Mediante RAPD's se analizaron 120 muestras foliares de 12 biotipos de Guadua angustifolia Kunth clasificados morfológicamente, procedentes de la cuenca del río Cauca, en el departamento del Cauca, Colombia, para determinar diversidad genética. El ADN se extrajo mediante el protocolo modificado de Dellaporta (1983. Se emplearon los cebadores; OPF-12, OPG-19, OPN-19 y OPP-16 con mayor número de bandas polimórficas. El índice de Shannon (HT = 0.4556 ± 0.1849 señaló diversidad genética total alta y diversidad entre los biotipos y al interior de ellos. El Índice de estructura genética (Gst = 0.5200 e Indice de migración efectiva (Nm = 0.4615 definieron biotipos bien diferenciados. El análisis de similaridad conformó tres grupos a un coeficiente de 0.64. El grupo G1 incluyó los biotipos Curvado, Rayada frecuente, Amarilla Playón, Rayada ancha, Rayada escasa, Convexa, Amarilla, Hembra, Verde irregular y algunos individuos de verde alta. El grupo G2, Verde alta y Macho. El grupo G3, Rayada negra. El estudio molecular agrupó los individuos de forma similar al estudio morfológico, con excepción de los individuos del biotipo Hembra.

    Palabras claves: Guadua angustifolia, caracterización molecular, variación genética.

  19. Comparación de metodologías moleculares para identificar el gen de la kappa caseína en ganado Holstein

    OpenAIRE

    Carlos Solarte P.; Carol Rosero G.; Yohana Eraso C.

    2012-01-01

    RESUMENObjetivo. Comparar las metodologías moleculares, PCR-RFLPs y PCR-SSCP, para identificar las variantes alélicas del gen de la kappa caseína (CSN3) en bovinos Holstein del trópico alto de Nariño-Colombia. Materiales y métodos. Se escogieron al azar 50 vacas Holstein y mediante punción en la vena coxígea media se tomaron muestras de 5cc de sangre, que se almacenaron y preservaron en tarjetas FTA® para su posterior análisis en el laboratorio. El ADN se amplificó por PCR utilizando cebadore...

  20. Complete epitopes for vaccine design derived from a crystal structure of the broadly neutralizing antibodies PGT128 and 8ANC195 in complex with an HIV-1 Env trimer.

    Science.gov (United States)

    Kong, Leopold; Torrents de la Peña, Alba; Deller, Marc C; Garces, Fernando; Sliepen, Kwinten; Hua, Yuanzi; Stanfield, Robyn L; Sanders, Rogier W; Wilson, Ian A

    2015-10-01

    The HIV-1 envelope gp160 glycoprotein (Env) is a trimer of gp120 and gp41 heterodimers that mediates cell entry and is the primary target of the humoral immune response. Broadly neutralizing antibodies (bNAbs) to HIV-1 have revealed multiple epitopes or sites of vulnerability, but mapping of most of these sites is incomplete owing to a paucity of structural information on the full epitope in the context of the Env trimer. Here, a crystal structure of the soluble BG505 SOSIP gp140 trimer at 4.6 Å resolution with the bNAbs 8ANC195 and PGT128 reveals additional interactions in comparison to previous antibody-gp120 structures. For 8ANC195, in addition to previously documented interactions with gp120, a substantial interface with gp41 is now elucidated that includes extensive interactions with the N637 glycan. Surprisingly, removal of the N637 glycan did not impact 8ANC195 affinity, suggesting that the antibody has evolved to accommodate this glycan without loss of binding energy. PGT128 indirectly affects the N262 glycan by a domino effect, in which PGT128 binds to the N301 glycan, which in turn interacts with and repositions the N262 glycan, thereby illustrating the important role of neighboring glycans on epitope conformation and stability. Comparisons with other Env trimer and gp120 structures support an induced conformation for glycan N262, suggesting that the glycan shield is allosterically modified upon PGT128 binding. These complete epitopes of two broadly neutralizing antibodies on the Env trimer can now be exploited for HIV-1 vaccine design. PMID:26457433

  1. Variabilidade genética de Eugenia uniflora L. em remanescentes florestais em diferentes estádios sucessionais

    Directory of Open Access Journals (Sweden)

    Roberto Valmorbida Aguiar

    2013-04-01

    Full Text Available A compreensão da diversidade genética fornece elementos básicos sobre a dinâmica e funcionamento de populações, auxiliando na conservação e uso sustentável das espécies. Supõe-se que populações sucessionais precoces poderiam ser geneticamente mais diferenciadas do que populações sucessionais mais tardias. Visando testar esta hipótese, o presente trabalho teve como objetivo analisar a variabilidade genética de populações de Eugenia uniflora L. em manchas florestais em diferentes estádios sucessionais. Foram selecionadas duas áreas em diferentes estádios de sucessão, sendo a primeira em estádio inicial e a segunda em estádio avançado. A área de estudo apresenta um remanescente florestal em transição de Floresta Ombrófila Mista e Floresta Estacional Semidecídua. Por meio da técnica de RAPD (Random Amplified Polymorphic DNA e análise multivariada, a diversidade gênica esperada e a porcentagem de loci polimórficos foram estimadas, além da similaridade genética entre as populações de cada mancha florestal e a diversidade de cada área por meio do índice de diversidade de Simpson. Os resultados indicaram 79% de loci polimórficos para a área em estádio avançado e 70% para a área em estádio inicial de sucessão. A similaridade genética entre pares de indivíduos variou entre 0,55 e 0,86 na área em estádio inicial de sucessão e entre 0,45 e 0,78 para a área em estádio avançado. Não houve diferenças significativas entre a diversidade das duas áreas (P = 89. Um escalonamento multidimensional não-métrico indicou menor distância genética entre os indivíduos da área em estádio inicial. Da mesma forma, uma análise de similaridade - ANOSIM indicou separação entre os indivíduos das duas áreas.

  2. Trifurcatia flabellata n. gen. n. sp., a putative monocotyledon angiosperm from the Lower Cretaceous Crato Formation (Brazil

    Directory of Open Access Journals (Sweden)

    B. Mohr

    2002-01-01

    Full Text Available The Lower Cretaceous Crato Formation (northeast Brazil contains plant remains, here described as Trifurcatia flabellata n. gen. and n. sp., consisting of shoot fragments with jointed trifurcate axes, each axis bearing a single amplexicaul serrate leaf at the apex. The leaves show a flabellate acrodromous to parallelodromous venation pattern, with several primary, secondary and higher order cross-veins. This very unique fossil taxon shares many characters with monocots. However, this fossil taxon exhibits additional features which point to a partly reduced, and specialized plant, which probably enabled this plant to grow in (seasonally dry, even salty environments. In der unterkretazischen Cratoformation (Nordostbrasilien sind Pflanzenfossilien erhalten, die hier als Trifurcatia flabellata n. gen. n. sp. beschrieben werden. Sie bestehen aus trifurcaten Achsen, mit einem apikalen amplexicaulen fächerförmigen serraten Blatt. Diese Blätter zeigen eine flabellate bis acrodrome-paralellodrome Aderung mit Haupt- und Nebenadern und transversale Adern 3. Ordnung. Diese Merkmale sind typisch für Monocotyledone. Allerdings weist dieses Taxon einige Merkmale auf, die weder bei rezenten noch fossilen Monocotyledonen beobachtet werden. Sie müssen als besondere Anpassungen an einen (saisonal trockenen und vielleicht übersalzenen Lebensraum dieser Pflanze interpretiert werden. doi:10.1002/mmng.20020050121

  3. The opgC gene is required for OPGs succinylation and is osmoregulated through RcsCDB and EnvZ/OmpR in the phytopathogen Dickeya dadantii.

    Science.gov (United States)

    Bontemps-Gallo, Sébastien; Madec, Edwige; Robbe-Masselot, Catherine; Souche, Erika; Dondeyne, Jacqueline; Lacroix, Jean-Marie

    2016-01-01

    Osmoregulated periplasmic glucans (OPGs) are a family of periplasmic oligosaccharides found in the envelope of most Proteobacteria. They are required for virulence of zoo- and phyto-pathogens. The glucose backbone of OPGs is substituted by various kinds of molecules depending on the species, O-succinyl residues being the most widely distributed. In our model, Dickeya dadantii, a phytopathogenic bacteria causing soft rot disease in a wide range of plant species, the backbone of OPGs is substituted by O-succinyl residues in media of high osmolarity and by O-acetyl residues whatever the osmolarity. The opgC gene encoding a transmembrane protein required for the succinylation of the OPGs in D. dadantii was found after an in silico search of a gene encoding a protein with the main characteristics recovered in the two previously characterized OpgC of E. coli and R. sphaeroides, i.e. 10 transmembrane segments and one acyl-transferase domain. Characterization of the opgC gene revealed that high osmolarity expression of the succinyl transferase is controlled by both the EnvZ-OmpR and RcsCDB phosphorelay systems. The loss of O-succinyl residue did not affect the virulence of D. dadantii, suggesting that only the glucose backbone of OPGs is required for virulence. PMID:26790533

  4. Phylogenetic analysis of env, gag, and tat genes of HIV type 1 detected among the injecting drug users in West Bengal, India.

    Science.gov (United States)

    Mullick, Ranajoy; Sengupta, Satarupa; Sarkar, Kamalesh; Saha, M K; Chakrabarti, Sekhar

    2006-12-01

    A recent occurrence of HIV-1 seropositivity among a group of injecting drug users (IDUs) in Darjeeling, a hilly district in northern West Bengal, revealed overall 11.8% HIV seroprevalence. Our study based on env (C2-V3), gag (p24-p7), and tat (exon-1) genomic regions of HIV-1 detected among this population showed that Darjeeling IDU sequences belonged to subtype C. Interestingly, the IDU sequences from Darjeeling were again found to be closer to the C strains from Manipur, a northeastern state in India, which is linked to the Golden Triangle via the Manipur-Myanmar border, rather than the IDU C sequences from Nepal, a neighboring country of India. The outgroup reference strains from different sites of IDU-driven epidemics in the world like Russia, Vietnam, Thailand, and Spain belonged to the nonsubtype C group and formed separate clusters from the subtype C cluster in our analysis. These results indicate a rapid spread of HIV-1 by possible drug trafficking along international boundaries, which might also help in the invasion of HIV-1 among IDUs of Darjeeling through the Manipur-Myanmar border of India. PMID:17209773

  5. Paring Down HIV Env: Design and Crystal Structure of a Stabilized Inner Domain of HIV-1 gp120 Displaying a Major ADCC Target of the A32 Region.

    Science.gov (United States)

    Tolbert, William D; Gohain, Neelakshi; Veillette, Maxime; Chapleau, Jean-Philippe; Orlandi, Chiara; Visciano, Maria L; Ebadi, Maryam; DeVico, Anthony L; Fouts, Timothy R; Finzi, Andrés; Lewis, George K; Pazgier, Marzena

    2016-05-01

    Evidence supports a role of antibody-dependent cellular cytotoxicity (ADCC) toward transitional epitopes in the first and second constant (C1-C2) regions of gp120 (A32-like epitopes) in preventing HIV-1 infection and in vaccine-induced protection. Here, we describe the first successful attempt at isolating the inner domain (ID) of gp120 as an independent molecule that encapsulates the A32-like region within a minimal structural unit of the HIV-1 Env. Through structure-based design, we developed ID2, which consists of the ID expressed independently of the outer domain and stabilized in the CD4-bound conformation by an inter-layer disulfide bond. ID2 expresses C1-C2 epitopes in the context of CD4-triggered full-length gp120 but without any known neutralizing epitope present. Thus, ID2 represents a novel probe for the analysis and/or selective induction of antibody responses to the A32 epitope region. We also present the crystal structure of ID2 complexed with mAb A32, which defines its epitope. PMID:27041594

  6. HIV-1 Env DNA vaccine plus protein boost delivered by EP expands B- and T-cell responses and neutralizing phenotype in vivo.

    Directory of Open Access Journals (Sweden)

    Kar Muthumani

    Full Text Available An effective HIV vaccine will most likely require the induction of strong T-cell responses, broadly neutralizing antibodies (bNAbs, and the elicitation of antibody-dependent cellular cytotoxicity (ADCC. Previously, we demonstrated the induction of strong HIV/SIV cellular immune responses in macaques and humans using synthetic consensus DNA immunogens delivered via adaptive electroporation (EP. However, the ability of this improved DNA approach to prime for relevant antibody responses has not been previously studied. Here, we investigate the immunogenicity of consensus DNA constructs encoding gp140 sequences from HIV-1 subtypes A, B, C and D in a DNA prime-protein boost vaccine regimen. Mice and guinea pigs were primed with single- and multi-clade DNA via EP and boosted with recombinant gp120 protein. Sera were analyzed for gp120 binding and induction of neutralizing antibody activity. Immunization with recombinant Env protein alone induced low-titer binding antibodies with limited neutralization breath. In contrast, the synthetic DNA prime-protein boost protocol induced significantly higher antibody binding titers. Furthermore, sera from DNA prime-protein boost groups were able to neutralize a broader range of viruses in a panel of tier 1 clade B viruses as well as multiple tier 1 clade A and clade C viruses. Further investigation of synthetic DNA prime plus adaptive EP plus protein boost appears warranted.

  7. Expression, refolding and preliminary X-ray crystallographic analysis of equine MHC class I molecule complexed with an EIAV-Env CTL epitope

    International Nuclear Information System (INIS)

    The equine MHC class I molecule was crystallized in complex with β2-microglobulin and a CTL epitope and X-ray diffraction data were collected to 2.3 Å resolution. In order to clarify the structure and the peptide-presentation characteristics of the equine major histocompatibility complex (MHC) class I molecule, a complex of equine MHC class I molecule (ELA-A1 haplotype, 7-6 allele) with mouse β2-microglobulin and the cytotoxic T lymphocyte (CTL) epitope Env-RW12 (RVEDVTNTAEYW) derived from equine infectious anaemia virus (EIAV) envelope protein (residues 195–206) was refolded and crystallized. The crystal, which belonged to space group P21, diffracted to 2.3 Å resolution and had unit-cell parameters a = 82.5, b = 71.4, c = 99.8 Å, β = 102.9°. The crystal structure contained two molecules in the asymmetric unit. These results should help to determine the first equine MHC class I molecule structure presenting an EIAV CTL epitope

  8. Immunologisches Reaktionsvermögen als Indikator für Belastungen bei der Milchkuh

    OpenAIRE

    Träbing, Claudia

    2006-01-01

    Mit ansteigenden Jahresmilchleistungen werden die Kühe einer hohen metabolischen Belastung ausgesetzt, die bei einer suboptimalen Gestaltung der Lebensbedingungen mit einer Erhöhung der Inzidenzrate von Faktorenkrankheiten einhergehen kann. Im Vordergrund der Untersuchungen stand die Frage, inwieweit metabolische Belastungszustände von Milchkühen in der Phase des Puerperiums und in Abhängigkeit von der Milchleistung sowie Belastungen durch eine klinische Erkrankung zu Beginn der Laktation mit...

  9. Análisis de la diversidad genética de 21 aislamientos del hongo Moniliophthora roreri basado en marcadores RAPD

    Directory of Open Access Journals (Sweden)

    Boris Gutarra Castillo

    2013-12-01

    Full Text Available Objetivos: Estudiar la diversidad genética de 21 aislamientos del hongo que afecta al cultivo del cacao, Moniliophthora roreri, en tres zonas cacaoteras del Perú (Tocache, Mariscal Cáceres y Leoncio Prado. Métodos: Se utilizó 14 iniciadores RAPD (random amplified polymorphic DNA polimórficos y una pareja de oligonucleótidos, los que fueron empleados bajo condiciones de amplificación estandarizadas. Con los datos obtenidos se construyó un dendograma utilizando el coeficiente de Jaccard y el algoritmo UPGMA (Unweighted Pair-Group Method using Arithmetic Average. La estructura genética fue estimada en función del análisis molecular de variancia (AMOVA y la diversidad mediante los índices de Shannon y Nei. Resultados: Fueron conseguidas 59 bandas RAPD con un 73% de polimorfismo. El dendograma obtenido a un índice de similitud de 0,70, claramente dividió los individuos en tres grupos. El análisis de la diversidad genética mostró altos valores en las zonas estudiadas de acuerdo con el índice de Shannon (0,3936 y de Nei (0,2622, con mayor riqueza en Leoncio Prado. Estas zonas presentan alta variabilidad, y según el AMOVA realizado: 88% entre accesiones por zona y solo 12% entre zonas. Conclusiones: Existe más de un grupo genético de Moniliophthora roreri en la Amazonía del Perú. Estos grupos, provenientes del Ecuador, pudieron haber ingresado por el intercambio de semillas y/o de forma natural por medio de los ríos en común y estarían originando nuevos grupos genéticos locales.

  10. Variabilidad genética de Moniliophthora perniciosa (Stahel Aime y Phillips-Mora, comb. nov. (Agaricales - Marasmiaceae en variedades de cacao (Theobroma cacao L.

    Directory of Open Access Journals (Sweden)

    Carolina Osorio-Solano

    2012-04-01

    Full Text Available Moniliophthora perniciosa, agente causante de la ‘escoba de bruja’ en cacao (Theobroma cacao, presenta una elevada variabilidad genética y discrepancias en su taxonomía y es una de las enfermedades más importantes en plantaciones cacaoteras que ocasiona pérdidas económicas a nivel mundial cercanas a 70%, y de 40% a nivel nacional. La caracterización de la diversidad genética de los biotipos es importante para la ejecución de proyectos encaminados al manejo de este patógeno y el desarrollo de materiales resistentes de cacao. En este estudio se analizaron 12 aislamientos del hongo obtenidos de diferentes materiales de cacao. Cada una de las muestras se evaluó con marcadores moleculares que tienen como blanco una región del ADN ribosomal (ADNr nuclear conocida como ITS (Internal Transcribed Spacer, una región intergénica (IGS-1 y cinco secuencias simples repetidas (SSR. El marcador IGS-1 permitió la determinación del biotipo C, no obstante se encontró una variabilidad genética evidente dentro de este biotipo, aún no registrada. El análisis de la diversidad genética de M. perniciosa por medio de marcadores microsatélite arrojó un valor total de 0.4260, una heterocigosidad total de 0.6143 y un índice de información polimórfica (PIC de 0.3407, valores considerados de rango medio a alto para los aislamientos estudiados y que estiman la variabilidad genética presente en M. perniciosa.

  11. Variabilidad genética de Moniliophthora perniciosa (Stahel Aime y Phillips-Mora, comb. nov. (Agaricales - Marasmiaceae en variedades de cacao (Theobroma cacao L.

    Directory of Open Access Journals (Sweden)

    Osorio Solano Carolina

    2012-11-01

    Full Text Available

    Moniliophthora perniciosa, agente causante de la 'escoba de bruja' en cacao (Theobroma cacao, presen- ta una elevada variabilidad genética y discrepancias en su taxonomía y es una de las enfermedades más importantes en plantaciones cacaoteras que ocasiona pérdidas económicas a nivel mundial cer- canas a 70%, y de 40% a nivel nacional. La caracterización de la diversidad genética de los biotipos es importante para la ejecución de proyectos encaminados al manejo de este patógeno y el desarrollo de materiales resistentes de cacao. En este estudio se analizaron 12 aislamientos del hongo obtenidos de diferentes materiales de cacao. Cada una de las muestras se evaluó con marcadores moleculares que tienen como blanco una región del ADN ribosomal (ADNr nuclear conocida como ITS (Internal Trans- cribed Spacer, una región intergénica (IGS-1 y cinco secuencias simples repetidas (SSR. El marcador IGS-1 permitió la determinación del biotipo C, no obstante se encontró una variabilidad genética evi- dente dentro de este biotipo, aún no registrada. El análisis de la diversidad genética de M. perniciosa por medio de marcadores microsatélite arrojó un valor total de 0.4260, una heterocigosidad total de

    0.6143 y un Indice de información polimórfica (PIC de 0.3407, valores considerados de rango medio a

    alto para los aislamientos estudiados y que estiman la variabilidad genética presente en M. perniciosa.

  12. Saúde coletiva, nova genética e a eugenia de mercado Collective health, the new genetics, and market eugenics

    Directory of Open Access Journals (Sweden)

    Maria Helena Cabral de Almeida Cardoso

    2003-04-01

    Full Text Available Com os constantes avanços das técnicas de manipulação genética, o campo da saúde coletiva passou a lidar com a possibilidade de surgimento de uma ênfase genômica reducionista, para além das influências sócio-culturais. Nestas circunstâncias, o foco principal das intervenções em saúde coletiva voltar-se-ia para a abordagem genômica de "indivíduos" e suas "famílias" em detrimento de seu consagrado objeto - "populações". Assim, seria deslocada a prioridade central dos esforços (e recursos para reduzir o adoecimento em função das desigualdades sócio-econômicas. Ao lado dos benefícios dos conhecimentos genéticos, há a possibilidade de surgimento de novas práticas eugênicas a partir da disponibilização de testagens genéticas no mercado, com vistas à aquisição pelos indivíduos interessados, desde que estes sejam capazes de atuarem como respectivos agentes de consumo.Due to constant advances in genetic manipulation, the field of public health is faced with the possible emergence of a reductionist genomic emphasis, beyond socio-cultural influences. Under such circumstances, the main focus of public health interventions would shift towards a genomic approach to "individuals" and their "families", to the detriment of their consecrated object - "populations", thereby moving away from the current central priority of efforts (and resources aimed at reducing ill-health due to socioeconomic inequalities. Even admitting the benefits of genetic knowledge, a new eugenic practice may emerge from the availability of genetic tests on the marketplace aimed at individuals that can afford to consume them.

  13. Makarnalık buğdayda (Triticum durum Desf.) Agrobacterium tumefaciens aracılığıyla gen aktarım frekansının artırılmasında gama radyasyondan yararlanma olanakları

    OpenAIRE

    2014-01-01

    Moleküler teknikler kullanılarak herhangi bir organizmadan izole edilen bir gen biyoteknolojik yöntemler aracılığıyla istenen bir organizmaya aktarılabilmektedir. Biyoteknolojik çalışmalarda bitkilere gen aktarımında Agrobacterium tumefaciens, mikroenjeksiyon ve partikül tabancası gibi çok sayıda gen aktarım yöntemi kullanılmasına karşın, Agrobacterium tumefaciens ile gen aktarımının çok önemli üstünlükleri bulunmaktadır. Agrobacter...

  14. NULIFE ASSOCIATION: An opportunity to face Gen II/Gen III challenges through cooperative research

    International Nuclear Information System (INIS)

    Excellence in roughly two years. It shall act as a facilitator to cooperative research. It shall: - Trigger innovation and promote new ideas; - Implement support to all Plant Life Management (PLIM) related issues. - Support the launching of new R and D projects; - Find and link funding partners and find R and D capabilities from the NULIFE competence pool; - Support project consortium agreement and management; - Support integration and harmonisation. Strategic research planning was started together with business plan and has been strongly related to the preparation of the First Chapter of the Strategic Research Agenda (SRA) of the Sustainable Nuclear Energy Technology Platform (SNE-TP). Many NULIFE organisations and members participate actively in the SNE-TP: - Governing Board: Chair, CEA, VTT, E.ON, EDF, Vattenfall, AREVA, SCK-CEN, TECNATOM, PSI, NRG,...; Executive Committee: Chair VTT; - Strategic Research Agenda Group: Chair of subgroup Gen2/Gen3, EDF; - Deployment Strategy Group: EDF. The First Chapter of the SNE-TP SRA defines the strategic targets in Long Term Operation (LTO), Performance improvement and External factors. In the Long Term Operation area, Safety justification, Ageing mechanisms of Systems-Structures-Components, Ageing monitoring and Prevention and mitigation of ageing are important subjects. In addition the SRA considers some cross-cutting areas like structural materials, prenormative research, codes and standards, modelling, simulation and methods. NULIFE is seen as a key instrument in implementing PLIM related topics of the SNE-TP strategy. The on-going and near future actions are on one side the preparation of road maps and specific short, medium and long term research topics to deploy the SRA, and on the other side to facilitate the launching of R and D projects on needs already shared by several stakeholders. Three projects are under construction on civil work, thermo-hydraulic code benchmark and warm pre-stress effect. Each project is supported

  15. Rendimento de grãos e qualidade tecnológica de genótipos de trigo em três zonas tritícolas do Estado de São Paulo no biênio 1994-95

    Directory of Open Access Journals (Sweden)

    FELICIO JOÃO CARLOS

    2000-01-01

    Full Text Available Foram avaliados o rendimento de grãos, a estabilidade e adaptabilidade ambiental, a tolerância ao alumínio tóxico e a qualidade tecnológica da farinha de vinte genótipos de trigo em três regiões tritícolas do Estado de São Paulo (Zonas A e B, sequeiro; Zona H, com irrigação por aspersão, no biênio 1994-95. Os resultados mostraram que: (a os genótipos tiveram comportamento regional diferenciado quanto ao rendimento de grãos; (b os genótipos IAC 289, IAC 340 e BR 18 mostraram-se, em média os mais produtivos, não diferindo do 'IAC 304', 'OCEPAR 14', 'IAC 350', 'Panda', 'IAC 60' e 'IAC 348'; (c os genótipos apresentaram, em geral, boa estabilidade produtiva nas diferentes condições ambientais; (d os genótipos IAC 289, IAC 340 e IAC 304 foram responsivos à melhoria ambiental e 'IAC 227', adaptado a ambientes desfavoráveis; (e os genótipos IAC 24, IAC 120, IAC 227, OCEPAR 14, IAC 348, Panda, BR 18, IAC 319, IAC 346, IAPAR 60, IAC 332 e IAC 347 foram tolerantes à toxicidade de Al3+; (f a farinha dos diferentes genótipos apresentou baixa atividade amilásica; (g os genótipos IAC 340, IAC 227, IAC 319 e IAC 60 propiciaram farinha com baixos valores de W (energia de deformação da massa e baixa estabilidade, características próprias de farinha de glúten fraco. Os demais genótipos revelaram característica de glúten forte.

  16. Reação de genótipos de soja ao alumínio em hidroponia e no solo Soybean genotypes reaction to aluminium in hydroponics and soil

    OpenAIRE

    Luiz Augusto Copati Souza

    2001-01-01

    O objetivo deste trabalho foi avaliar os genótipos de soja BR86-5974, BR86-7396, Dourados, Doko RC, EMGOPA 305, IAC-9, BR-9 (Savana), UFV-1, UFV-9 e UFV Araguaia em relação à tolerância ao alumínio (Al) em hidroponia e em solo. Na solução com Al foi medido o comprimento radicular. Em solo com 49% de saturação de Al avaliou-se área foliar, altura de planta, altura de inserção da primeira vagem, produção de matéria seca, produção de grãos e índice de colheita. Os genótipos BR86-7396 e IAC-9 são...

  17. GenGIS: A geospatial information system for genomic data

    OpenAIRE

    Donovan H. Parks; Porter, Michael; Churcher, Sylvia; Wang, Suwen; Blouin, Christian; Whalley, Jacqueline; Brooks,Stephen; Robert G Beiko

    2009-01-01

    The increasing availability of genetic sequence data associated with explicit geographic and ecological information is offering new opportunities to study the processes that shape biodiversity. The generation and testing of hypotheses using these data sets requires effective tools for mathematical and visual analysis that can integrate digital maps, ecological data, and large genetic, genomic, or metagenomic data sets. GenGIS is a free and open-source software package that supports the integr...

  18. Luminescence of MBE SimGen strained monolayer superlattices

    International Nuclear Information System (INIS)

    This paper reports on SimGen strained monolayer superlattices (SMS) that have been fabricated by molecular beam epitaxy (MBE) and characterized using photoluminescence (PL). Symmetrically strained structures with different periodicities have been grown on top of a Si1-xGex alloy buffer layer. Luminescence features below (above) the Si(Ge) energy bandgap have been observed and attributed to either dislocations in the buffer layer or to energy band transitions in the SMS

  19. Radio Transmission Performance of EPCglobal Gen-2 RFID System

    CERN Document Server

    Mohaisen, Manar; Chang, KyungHi

    2009-01-01

    In this paper, we analyze the performance of the encoding and the modulation processes in the downlink and uplink of the EPCglobal Gen2 system through the analysis and simulation. Furthermore, the synchronization issues on time and frequency domain and the preamble architecture are evaluated. Through the simulation in the uplink, we find that the detection probability of FM0 and Miller coding approaches 1 at 13dB Eb/N0.

  20. GenBank blastx search result: AK243016 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK243016 J090098K14 AB088119.1 AB088119 Streptomyces sp. TP-A0274 staurosporine biosynthetic gen ... , staB, staA, staN, staG, staO, staD, staP, staMA, staJ , staK, staI, staE, staMB), complete cds. BCT 3e-23 ...

  1. AL Amyloidosis

    Directory of Open Access Journals (Sweden)

    Desport Estelle

    2012-08-01

    Full Text Available Abstract Definition of the disease AL amyloidosis results from extra-cellular deposition of fibril-forming monoclonal immunoglobulin (Ig light chains (LC (most commonly of lambda isotype usually secreted by a small plasma cell clone. Most patients have evidence of isolated monoclonal gammopathy or smoldering myeloma, and the occurrence of AL amyloidosis in patients with symptomatic multiple myeloma or other B-cell lymphoproliferative disorders is unusual. The key event in the development of AL amyloidosis is the change in the secondary or tertiary structure of an abnormal monoclonal LC, which results in instable conformation. This conformational change is responsible for abnormal folding of the LC, rich in β leaves, which assemble into monomers that stack together to form amyloid fibrils. Epidemiology AL amyloidosis is the most common type of systemic amyloidois in developed countries with an estimated incidence of 9 cases/million inhabitant/year. The average age of diagnosed patients is 65 years and less than 10% of patients are under 50. Clinical description The clinical presentation is protean, because of the wide number of tissues or organs that may be affected. The most common presenting symptoms are asthenia and dyspnoea, which are poorly specific and may account for delayed diagnosis. Renal manifestations are the most frequent, affecting two thirds of patients at presentation. They are characterized by heavy proteinuria, with nephrotic syndrome and impaired renal function in half of the patients. Heart involvement, which is present at diagnosis in more than 50% of patients, leading to restrictive cardiopathy, is the most serious complication and engages prognosis. Diagnostic methods The diagnosis relies on pathological examination of an involved site showing Congo red-positive amyloid deposits, with typical apple-green birefringence under polarized light, that stain positive with an anti-LC antibody by immunohistochemistry and

  2. Entrenamiento y evaluación de una competencia genérica para los títulos de grado universitarios

    OpenAIRE

    Rodríguez, Margarita; Gutiérrez, Juan J.; Beltrán, Juan F.; Conradi, Mercedes

    2011-01-01

    Este trabajo surge ante la necesidad de entrenar y evaluar una competencia genérica en la materia de Zoología del Grado de Biología “Solidez en los conocimientos básicos de la profesión”. Consideramos que es fundamental que los estudiantes adquieran conocimientos suficientes para valorar la importancia de los aspectos genéricos que poseen los animales tales como, morfología, anatomía, histología, fisiología, embriología, etología y ecología. Al ser una asignatura difícil y poco atractiva para...

  3. Hacking the code : una aproximació indagadora a l'ensenyament del codi genètic, o seguint les passes de Nirenberg i Khorana

    OpenAIRE

    Domènech Casal, Jordi

    2013-01-01

    Es descriu el disseny i aplicació d'una activitat didàctica sobre el codi genètic, al llarg de tres eixos didàctics: l'aprenentatge per indagació, la naturalesa del coneixement científic i la reflexió sobre els aprenentatges. L'activitat ressegueix l'estratègia dels experiments rea-litzats per Nirenberg i Khorana per a descobrir el codi genètic i la seva aplicació mostra que és un bon punt de partida per a introduir el dogma central de la biologia molecular, que defensem que és un aspecte imp...

  4. “Eugenesia, normatividad jurídica y sociedad tecnológica. Retos bioéticos de la nueva genética”

    Directory of Open Access Journals (Sweden)

    Javier Blazquez

    2009-07-01

    Full Text Available Las posibilidades que ofrece la investigación y experimentación genética son de gran relevancia. Así como los retos y desafíos de carácter jurídico que plantean. Retos que interpelan y demandan una regulación que evite que el caudal y potencial de la investigación pueda llegar a desbordarse. Otra cosa es ponderar y crear normas capaces de optimizar al máximo los recursos que aporta la tecnociencia y la experimentación genética, haciéndolos compatibles con el respeto a los Derechos Humanos concernidos. Ese es uno de los objetivos del Convenio de Biomedicina del Consejo de Europa así como de la Ley 14/2007 de 3 de julio,  de Investigación  Biomédica.

  5. The GenABEL Project for statistical genomics.

    Science.gov (United States)

    Karssen, Lennart C; van Duijn, Cornelia M; Aulchenko, Yurii S

    2016-01-01

    Development of free/libre open source software is usually done by a community of people with an interest in the tool. For scientific software, however, this is less often the case. Most scientific software is written by only a few authors, often a student working on a thesis. Once the paper describing the tool has been published, the tool is no longer developed further and is left to its own device. Here we describe the broad, multidisciplinary community we formed around a set of tools for statistical genomics. The GenABEL project for statistical omics actively promotes open interdisciplinary development of statistical methodology and its implementation in efficient and user-friendly software under an open source licence. The software tools developed withing the project collectively make up the GenABEL suite, which currently consists of eleven tools. The open framework of the project actively encourages involvement of the community in all stages, from formulation of methodological ideas to application of software to specific data sets. A web forum is used to channel user questions and discussions, further promoting the use of the GenABEL suite. Developer discussions take place on a dedicated mailing list, and development is further supported by robust development practices including use of public version control, code review and continuous integration. Use of this open science model attracts contributions from users and developers outside the "core team", facilitating agile statistical omics methodology development and fast dissemination. PMID:27347381

  6. Cosmosperma polyloba gen. et sp. nov., a seed plant from the Upper Devonian of South China

    Science.gov (United States)

    Wang, Deming; Liu, Le; Meng, Meicen; Xue, Jinzhuang; Liu, Tuo; Guo, Yun

    2014-08-01

    Seed plants with ovules were abundant in the Late Devonian of Euramerica and they contribute significantly to our understanding of their early history. However, coeval ovules have been scarce in other regions of the world. Specimens of the seed plant Cosmosperma polyloba gen. et sp. nov. Wang et al. were recently obtained from the Upper Devonian (Famennian) Wutong Formation, at Fanwan Village, Changxing County, Zhejiang Province, China. This new seed plant has cupulate ovules, the uniovulate cupules with up to 16 distal segments and with minute spines on the outer surface, synangiate pollen organs bearing six to eight microsporangia fused only at the base, and planate and highly dissected pinnules in alternate arrangement. It differs from other Devonian seed plants mainly in the organization and position of the uniovulate and ornamented cupule, and in the highly dissected pinnules. Cosmosperma Wang et al. represents the first Devonian ovules recovered from China or eastern Asia and further illustrates the diversity of early spermatophytes. As for the Late Devonian seed plants, it is suggested that the pollen organs are synangiate and simple in organization, and the branches and leaves are generally planate.

  7. Variabilidad genética de Aedes aegypti en algunas áreas del Perú usando Single Stranded Conformational Polymorphism (SSCP

    Directory of Open Access Journals (Sweden)

    Nélida Leiva G

    2004-07-01

    Full Text Available Aedes aegypti es el vector responsable de la transmisión del virus del dengue, su distribución geográfica se ha ampliado rápidamente debido principalmente a la intervención de los seres humanos. Objetivo: Analizar la variabilidad genética de este mosquito mediante la comparación del Segundo Espaciador Transcrito Interno (ITS 2 perteneciente al ADN ribosomal (rADN. Materiales y Métodos: Se analizaron muestras de ocho localidades (Jaén, Tingo María, Iquitos, Lambayeque, el distrito de El Rimac, Sullana y Zarumilla y uno de la provincia de Huaquillas (Ecuador. El análisis de la variabilidad se determinó usando la técnica conocida como SSCP (Single Stranded Conformation Polymorphism. Resultados: El estudio muestra que existe variabilidad genética entre las poblaciones analizadas, principalmente entre las muestras localizadas en la costa del Perú (Zarumilla, El Rímac, Sullana y Huaquillas y las muestras del nororiente (Tingo María, Iquitos, Jaén y Lambayeque Conclusión: Se determinaron dos variantes genéticas entre las poblaciones de Aedes aegypti: Costeña y Nororiental, que probablemente provienen de dos ancestros diferentes y cuyo ancestro común sufrió de aislamiento por distancia. Se observó que no existe relación entre las distancias genéticas y las distancias geográficas indicando que la migración de estas poblaciones es el resultado de la intervención de los seres humanos que diseminan al vector y no por la migración activa del mosquito. Se plantea el papel de la Cordillera de los Andes en la migración y separación de las poblaciones de Aedes.

  8. Búsqueda de genes de resistencia al pasador del fruto en poblaciones segregantes de tomate

    OpenAIRE

    Diana Milena Rodríguez Mora; Jhon Fredy Betancur-Pérez; Franco Alirio Vallejo Cabrera; Juan Carlos Vaca-Vaca; Karina López-López

    2010-01-01

    Las estrategias de mejoramiento genético para encontrar resistencia al pasador del fruto, Neoleucinodes elegantalis, en tomate, Solanum lycopersicum L. se han basado en métodos tradicionales de introgresión genética por medio de retrocruzamiento. Sin embargo este proceso es largo y dispendioso. Debido a esta problemática, se utilizó la técnica molecular cDNA-AFLP para identificar genes involucrados en la resistencia a esta plaga en poblacione...

  9. Pantanalinema gen. nov. and Alkalinema gen. nov.: novel pseudanabaenacean genera (Cyanobacteria) isolated from saline-alkaline lakes.

    Science.gov (United States)

    Vieira Vaz, Marcelo Gomes Marçal; Genuário, Diego Bonaldo; Andreote, Ana Paula Dini; Malone, Camila Francieli Silva; Sant'Anna, Célia Leite; Barbiero, Laurent; Fiore, Marli Fátima

    2015-01-01

    The genus Leptolyngbya Anagnostidis & Komárek (1988) was described from a set of strains identified as 'LPP-group B'. The morphology within this group is not particularly informative and underestimates the group's genetic diversity. In the present study, two new pseudanabaenacean genera related to Leptolyngbya morphotypes, Pantanalinema gen. nov. and Alkalinema gen. nov., are described under the provisions of the International Code of Nomenclature for Algae, Fungi and Plants, based on a polyphasic approach. Pantanalinema gen. nov. (type species Pantanalinema rosaneae sp. nov.) has sheaths and trichomes with slight gliding motility, which distinguish this genus from Alkalinema gen. nov. (type species Alkalinema pantanalense sp. nov.), which possesses trichomes arranged in an ornate (interwoven) pattern. 16S rRNA gene sequences of strains of Pantanalinema and Alkalinema exhibited low identity to each other (≤91.6 %) and to other sequences from known pseudanabaenacean genera (≤94.3 and 93.7 %, respectively). In a phylogenetic reconstruction, six sequences from strains of Pantanalinema and four from strains of Alkalinema formed two separate and robust clades (99 % bootstrap value), with the genera Oculatella and Phormidesmis, respectively, as the closest related groups. 16S-23S rRNA intergenic spacer sequences and secondary structures of strains of Pantanalinema and Alkalinema did not correspond to any previous descriptions. The strains of Pantanalinema and Alkalinema were able to survive and produce biomass at a range of pH (pH 4-11) and were also able to alter the culture medium to pH values ranging from pH 8.4 to 9.9. These data indicate that cyanobacterial communities in underexplored environments, such as the Pantanal wetlands, are promising sources of novel taxa. PMID:25351877

  10. J3Gen: A PRNG for Low-Cost Passive RFID

    Science.gov (United States)

    Melià-Seguí, Joan; Garcia-Alfaro, Joaquin; Herrera-Joancomartí, Jordi

    2013-01-01

    Pseudorandom number generation (PRNG) is the main security tool in low-cost passive radio-frequency identification (RFID) technologies, such as EPC Gen2. We present a lightweight PRNG design for low-cost passive RFID tags, named J3Gen. J3Gen is based on a linear feedback shift register (LFSR) configured with multiple feedback polynomials. The polynomials are alternated during the generation of sequences via a physical source of randomness. J3Gen successfully handles the inherent linearity of LFSR based PRNGs and satisfies the statistical requirements imposed by the EPC Gen2 standard. A hardware implementation of J3Gen is presented and evaluated with regard to different design parameters, defining the key-equivalence security and nonlinearity of the design. The results of a SPICE simulation confirm the power-consumption suitability of the proposal. PMID:23519344

  11. A Frequency Diverse Gen2 RFID System with Isolated Continuous Wave Emitters

    Directory of Open Access Journals (Sweden)

    Hsin-Chin Liu

    2007-09-01

    Full Text Available The “Gen2” specification for UHF passive RFID systems released by EPCglobal has become an intense research interest. A Gen2 tag derives its power from the RF wave emitted by a Gen2 RFID reader and responds its modulated backscatter signals to the reader. Due to the large propagation loss, the accessible range of a Gen2 tag is hence limited. Moreover, the readability of a Gen2 tag is often influenced by the multipath fading problem. In order to mitigate the problems, a multi-carrier UHF passive RFID system utilizing the frequency diverse backscatter ability of a Gen2 tag is proposed in a prior work. In this work, a thorough analysis of the system is given. Especially, the appropriate powers of the transceiver and CWEs are derived to make the system more feasible in practical applications.

  12. Fusion of the upstream vpu sequences to the env of simian human immunodeficiency virus (SHIVKU-1bMC33) results in the synthesis of two envelope precursor proteins, increased numbers of virus particles associated with the cell surface and is pathogenic for pig-tailed macaques

    International Nuclear Information System (INIS)

    Previous studies have shown that the gene coding for the Vpu protein of the human immunodeficiency virus type 1 (HIV-1) is 5' to the env gene, is in a different reading frame, and overlaps the env by 90 nucleotides. In this study, we examined the processing of the Env protein as well as the maturation and infectivity of a virus (SHIVVpenv) in which a single nucleotide was removed at the vpu-env junction, fusing the first 162 bases of vpu to the env ORF. Pulse-chase analysis revealed that SHIVVpenv-infected cells gave rise to two precursor glycoprotein species (gp160 and gp175). Immune precipitation results also revealed that an anti-Vpu serum could immune precipitate the gp175 precursor, suggesting that the amino-terminal Vpu sequence was fused to the Env protein. Growth curves revealed that the SHIVVpenv-inoculated cultures released approximately three times more p27 into the culture medium than parental SHIVKU-1bMC33. Electron microscopy revealed that while both viruses matured at the cell plasma membrane, significantly higher quantities of virus particles were cell associated on SHIVVpenv-infected cells compared to cultures inoculated with parental SHIVKU-1bMC33. Furthermore, virus was observed maturing into intracellular vesicles of SHIVVpenv-infected cells. To assess the pathogenicity of SHIVVpenv, three pig-tailed macaques were inoculated with the SHIVVpenv and monitored for 6 months for CD4+ T cell levels, viral loads, and the stability of the deletion at the vpu-env junction. Our results indicated that SHIVVpenv caused a severe CD4+ T cell loss in all three macaques within weeks of inoculation. Sequence analysis of the vpu gene analyzed from sequential PBMC samples derived from macaques revealed that this mutation was stable during the period of rapid CD4+ T cell loss. Sequence analysis showed that with increasing time of infection, the one base pair deletion was repaired in all three macaques inoculated with SHIVVpenv with the reversion occurring at 10

  13. Hist??ria natural de les malalties gen??tiques mendelianes i complexes

    OpenAIRE

    Lao Grueso, Oscar, 1976-

    2004-01-01

    Las enfermedades gen??ticas se clasifican t??picamente en dos grandes grupos: las enfermedades mendelianas y las enfermedades complejas. Mientras que las enfermedades mendelianas se caracterizan por ser de baja frecuencia en la poblaci??n y estar causadas por mutaciones en un gen particular, las enfermedades complejas son el principal problema sanitario en los pa??ses desarrollados y se encuentran producidas por la interacci??n de factores ambientales y factores gen??ticos. En este caso no se...

  14. Crucigrama de conceptos generales de Genética. Nivel semi-avanzado.

    OpenAIRE

    Cross, Ismael; Rebordinos, Laureana

    2012-01-01

    El archivo es un ejercicio desarrollado con JCross en Hotpotatoes para que los alumnos prueben e identifiquen su nivel con respecto a cuestiones de Genética, conceptos, técnicas, características del ADN, etc. El ejercicio permite obtener pistas para completar el crucigrama y de esta manera finalizar el mismo habiendo asimilado o aprendido conceptos ya sabidos o nuevos de Genética. El crucigrama contiene cerca de 40 definiciones de Genética.

  15. El envío de indios americanos a la Península Ibérica: aspectos legales (1492-1542

    Directory of Open Access Journals (Sweden)

    Esteban MIRA CABALLOS

    2009-12-01

    Full Text Available RESUMEN: En este presente artículo ofrecemos por primera vez una sistematización sobre la legislación existente en torno a la trata de indios americanos con destino a los mercados esclavistas de la Península Ibérica. Se trata sin duda de un trabajo sumamente novedoso que aporta nueva luz a un aspecto de la Historia Moderna de España que ha pasado casi desapercibido hasta la fecha para la historiografía más reciente. La conclusión principal que se puede extraer de este trabajo es la negativa de la Corona -salvo en los dubitativos momentos iniciales- a que se trajesen indios esclavos a la Península. No obstante, esta legislación prohibitiva sólo sirvió para que los envíos se ralentizaran sin erradicar totalmente una trata que siempre encontró las formas de continuar de una manera más o menos fraudulenta.ABSTRACT: For the first time the present article offers us a systematization about the existing laws concerning the American indians trade with the Iberian Peninsula slaves markets. Undoubtledly, it consists of an extremely original work which casts light on one aspect of the Spanish early modern history that has gone unnoticed to the recent historiography. The main conclusion to be drawn from this work is the Crown's denial for the Indian slaves to be brought, except for the doubts arisen at the beginning. This prohibitive laws only served to slow down the slave shipment without eradicate this practice that always founds to continue new forms, more or less fraudulent.

  16. Evaluation of moisture and heat transport in the fast-response building-resolving urban transport code QUIC EnvSim

    Science.gov (United States)

    Briggs, Kevin A.

    QUIC EnvSim (QES) is a complete building-resolving urban microclimate modeling system developed to rapidly compute mass, momentum, and heat transport for the design of sustainable cities. One of the more computationally intensive components of this type of modeling system is the transport and dispersion of scalars. In this paper, we describe and evaluate QESTransport, a Reynolds-averaged Navier-Stokes (RANS) scalar transport model. QESTransport makes use of light-weight methods and modeling techniques. It is parallelized for Graphics Processing Units (GPUs), utilizing NVIDIA's OptiX application programming interfaces (APIs). QESTransport is coupled with the well-validated QUIC Dispersion Modeling system. To couple the models, a new methodology was implemented to efficiently prescribe surface flux boundary conditions on both vertical walls and flat surfaces. In addition, a new internal boundary layer parameterization was introduced into QUIC to enable the representation of momentum advection across changing surface conditions. QESTransport is validated against the following three experimental test cases designed to evaluate the model's performance under idealized conditions: (i) flow over a step change in moisture, roughness, and temperature, (ii) flow over an isolated heated building, and (iii) flow through an array of heated buildings. For all three cases, the model is compared against published simulation results. QESTransport produces velocity, temperature, and moisture fields that are comparable to much more complex numerical models for each case. The code execution time performance is evaluated and demonstrates linear scaling on a single GPU for problem sizes up to 4.5 x 4.5 km at 5 m grid resolution, and is found to produce results at much better than real time for a 1.2 x 1.2 km section of downtown Salt Lake City, Utah.

  17. Nova metodologia de avaliação da reação de genótipos de capim-colonião ao alumínio

    Directory of Open Access Journals (Sweden)

    OLIVEIRA ANTONIO CARLOS DE

    2000-01-01

    Full Text Available Foi definida nova metodologia de avaliação de genótipos quanto à reação ao alumínio (Al3+, presente em soluções nutritivas, através da associação de análises de componentes principais e de estabilidade/adaptabilidade fenotípicas. Os experimentos foram instalados em delineamentos de blocos ao acaso, em parcelas subdivididas, com três repetições e conduzidos em casa de vegetação da Seção de Genética - Centro Experimental de Campinas/IAC, no período de 1995 a 1997. Em diferentes genótipos de capim-colonião (Panicum maximum Jacq. foram avaliados caracteres de sistema radicular e de parte aérea, sob três concentrações de Al3+ (zero, 12 e 24 mg/L e três períodos de permanência em solução (15, 30 e 45 dias. Reduções porcentuais, causadas pela presença do Al3+, foram calculadas para cada caráter analisado e, a partir delas, efetuadas as análises acima citadas. Considerável precisão foi observada na diferenciação dos genótipos quanto ao estresse, sugerindo o seu emprego em programas de melhoramento genético.

  18. Construction of DNA vaccine expressing codon-modified HIV-1 consensus env gene derived from subtype CRF01_AE infected individuals in China%HIV-1中国流行株CRF01_AE env基因改造及其重组DNA疫苗的构建

    Institute of Scientific and Technical Information of China (English)

    陈丹瑛; 汪孟冉; 何小周; 叶景荣; 余双庆; 李秦剑; 徐柯; 曾毅; 冯霞

    2014-01-01

    Objective To construct a DNA vaccine containing codon-modified HIV-1 consensus env gene which will be a candidate vaccine in multiple vector-based therapeutic AIDS vaccine strategy.Methods HIV-1 CRF01_AE env genes were amplified using subtype-specific primmer sets and cloned to pcDNA 3.1.The consensus sequence was acquired by alignment of all the env sequences with software.Then codons of the consensus env sequence were modified according to mammalian codon usage.The modified env gene mod.AE env was cloned into pVR vector to get DNA vaccine pVR-mod.AE env.The expression level of wild type and codon-modified env gene was analyzed by Western Blot assay.Results Phylogenetic analysis of the full-length env nucleotide sequences confirmed that all 32 isolates were grouped within CRF01_AE.The DNA vaccine expressing the codon-modified consensus env gene derived from these 32 sequences was constructed successfully.The codon modification increased the expression level of Env protein significantly.Conclusion The codon modification of CRF01_AE env gene and construction of DNA vaccine expressing this gene was successful.%目的 构建表达含有密码子优化的HIV-1 CRF01_AE亚型env基因的DNA疫苗,为多载体艾滋病治疗性疫苗的应用提供候选疫苗.方法 对HIV-1感染者血液样本进行型别分析,分型得到的AE亚型标本采用亚型特异性引物克隆env基因,通过序列比对获得其共有序列,对该序列按照哺乳动物密码子使用的偏嗜性进行优化,将人工合成的优化基因克隆至pVR载体,构建DNA疫苗.通过Western Blot方法比较优化前后env基因的表达.结果 成功获得32条具有完整的开放性阅读框的env克隆,型别分析确认均为CRF01_AE亚型.成功对其共有序列进行了优化、合成并构建了DNA疫苗pVR-mod.AE env,该疫苗与野生型的env基因(wt.AE env)相比可以高水平表达env基因,且不依赖Rev的存在.结论 对HIV-1中国流行株CRF01_AE env基因的优化改

  19. Reflexos da interação genótipo X ambiente e suas implicações nos ganhos de seleção em genótipos de feijão (Phaseolus vulgaris L. Reflexes of the interaction genotype X environment and their implications in the gains of selection in genotypes of bean (Phaseolus vulgaris L.

    Directory of Open Access Journals (Sweden)

    Jefferson Luís Meirelles Coimbra

    1999-09-01

    Full Text Available A importância das leguminosas de grãos na alimentação humana, principalmente do feijão preto (Phaseolus vulgaris, tem estimulado os melhoristas a selecionar genótipos com alto potencial de rendimento de grãos e com adaptabilidade às diferentes condições de cultivo do sul do Brasil. O presente trabalho foi realizado com o objetivo de avaliar os reflexos da interação genótipo x ambiente e suas implicações nos ganhos genéticos com diferentes critérios de seleção. Os resultados revelaram que o componente da interação genótipo x ambiente superestima a predição dos parâmetros genéticos, como por exemplo a variância genética e a herdabilidade. As diferenças observadas entre estas estimativas parecem ocorrer devido à alta percentagem da parte complexa da interação. Além disto, os ganhos genéticos obtidos com a seleção direta foram sempre superiores à resposta indireta. Comparativamente, o par de ambientes 1x3 revelou uma resposta correlacionada inferior e de sinal contrário às demais estimativas para os outros pares de ambientes estudados neste trabalho. O primeiro ambiente foi o que mais acumulou a interação genótipo x ambiente. Portanto, pode ser concluído que o componente da interação tem grande relevância nas estimativas dos ganhos genéticos, evidenciando que essa influência deva ser considerada na seleção e na recomendação de genótipos específicos nos programas de melhoramento genético da cultura do feijoeiro.The importance of grains of legume plants for human feeding, specially black beans (Phaseolus vulgaris L., has stimulated the breeders to select genotypes with high grains yield potential and wide adaptability to different conditions of cultivation in southern Brazil. The present work aimed at evaluating the reflexes of the genotype x environment interaction and its implications in the genetic gains of different selection approaches. The results revealed that the component of the

  20. Las mutaciones inestables, nuevo reto para el consejo genético de enfermedades hereditarias

    Directory of Open Access Journals (Sweden)

    Patricia Cuenca

    2004-09-01

    Full Text Available Las mutaciones inestables constituyen un tipo de alteración genética descubierta en la década de los noventa. En condiciones normales, regiones específicas de los genes están constituidas por repeticiones de una secuencia corta que puede ser de tres, cuatro, cinco o más nucleótidos; por ejemplo CAG, CGG, ATTCT, etc. Este nuevo tipo de mutación consiste en un aumento en la cantidad de éstas repeticiones, lo que causa una alteración en la expresión de dichos genes. Son inestables porque se ha observado que el tamaño de la secuencia repetida varía cuando las células se dividen por mitosis o meiosis, lo cual tiene implicaciones sobre la herencia y por consiguiente sobre el consejo genético que debe brindarse a los afectados. Estas mutaciones se han encontrado en genes importantes para la función neurológica normal del ser humano, donde pueden alterar el transporte de los ARN desde el núcleo al citoplasma, provocar la inactivación del gen o producir una proteína con funciones nuevas. La mayoría de las enfermedades causadas por estas mutaciones afectan el sistema nervioso, son degenerativas y presentan el fenómeno de anticipación genética, es decir que los afectados dentro de una familia se enfermarán más jóvenes y en forma más severa con el paso de las generaciones. La cantidad de repeticiones de la secuencia repetida presenta una correlación negativa con la edad de manifestación, así como una correlación positiva con la severidad de la enfermedad. En este momento existen veinte padecimientos identificados que son causados exclusivamente por este tipo de mutación. Este trabajo es una breve revisión actualizada del tema.Unstable mutations, new challenges for genetic counseling of inherited disorders. Unstable mutations or amplification of DNA tandem repeats sequences constitute a new kind of genetic alteration discovered in the 90´s that cause hereditary diseases. This mutation has been found inside or near important

  1. Seleção de genótipos parentais de acerola com base na divergência genética multivariada

    Directory of Open Access Journals (Sweden)

    CARPENTIERI-PÍPOLO VALÉRIA

    2000-01-01

    Full Text Available Este trabalho teve por objetivo identificar e selecionar genótipos parentais de acerola (Malpighia emarginata L. adequadas a programas de melhoramento genético. Nove caracteres quantitativos de maior importância agronômica foram usados para determinação da distância genética e formação de grupos similares de acessos. O agrupamento pelo método de Tocher, a partir das distâncias generalizadas de Mahalanobis, possibilitou a divisão de 14 genótipos em três grupos. Com base na divergência genética e no caráter agronômico-chave (teor de vitamina C, destacaram-se como mais promissores os cruzamentos dos genótipos: AM Mole pertencente ao grupo III, com os genótipos PR AM, N° 18, PR 17, PR 16, Eclipse, AM 22 e Dominga, todos pertencentes ao grupo I.

  2. GenBank blastx search result: AK058388 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK058388 001-015-A02 AF220294.1 Mus musculus clone BAC126c8 Rsp29-like protein (Rsp29) and Als ... s ... plice variant 2 (Als ) genes, partial cds; Als ... splice variant 1 (Als ), T ...

  3. GenBank blastx search result: AK061858 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK061858 001-040-G07 AF220294.1 Mus musculus clone BAC126c8 Rsp29-like protein (Rsp29) and Als ... s ... plice variant 2 (Als ) genes, partial cds; Als ... splice variant 1 (Als ), T ...

  4. GenBank blastx search result: AK287750 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK287750 J065155L15 AF220294.1 AF220294 Mus musculus clone BAC126c8 Rsp29-like protein (Rsp29) a ... nd Als ... splice variant 2 (Als ) genes, partial cds; Als ... spl ... ice variant 1 (Als ), TCE2 (Tce2), NDK3-like protein (Ndk3), and TCE4 ...

  5. La Red Nacional de Ensayos Genéticos: una herramienta para la monitorización de la diversidad genética

    OpenAIRE

    Chambel, María Regina; Notivol Paíno, Eduardo; Barba, Diana; Caño, F. del; Climent, José; Alía Miranda, Ricardo

    2013-01-01

    La diversidad genética es un componente esencial de la biodiversidad. Mientras que las actuales técnicas de secuenciación permiten evaluar la diversidad genética neutral a un bajo coste, la evaluación de la diversidad genética en caracteres cuantitativos (de gran importancia adaptativa) constituirá en un futuro próximo la principal limitación pues sigue necesitando ensayos en ambiente común y condiciones ecológicas contrastadas, además de un seguimiento permanente. La R...

  6. Heredabilidad y valor genético (reml/blup en genotipos de un eucalipto tolerante a la sequía, en el norte de Chile.

    Directory of Open Access Journals (Sweden)

    Freddy Mora

    2010-08-01

    Full Text Available Escasas especies del género Eucalyptus son consideradas útiles para la forestación en zonas secas. Una de ellas es Eucalyptus cladocalyx, que además está siendo considerada en programas de hibridación interespecífica para mejorar características como la tolerancia al déficit hídrico. 49 genotipos (familias de medio hermanos, provenientes de seis poblaciones, fueron evaluadas a los 30 meses después de la plantación, en dos localidades del semiárido Chileno: Caracas y Tunga. Cada uno de los ensayos tuvo un diseño de bloques completos al azar, con parcelas de un árbol, con 30 bloques. Se estimaron componentes de varianza por el método de máxima verosimilitud restringida (REML en un modelo lineal mixto. Se obtuvieron los valores genéticos a través de la mejor predicción lineal no sesgada (BLUP del efecto aleatorio debido al origen (poblaciones. A pesar de que el cálculo de la heredabilidad fue conservador al considerar un coeficiente k = 2,5, las heredabilidades de la altura fueron moderadas y altas, con valores desde 0,48 en Caracas, a 0,76 en Tunga. Los valores de la heredabilidad para el diámetro basal fueron moderados, variando desde 0,26 a 0,39 en Caracas y Tunga, respectivamente. Los orígenes de Cowell, Remarkable e Illapel, mostraron valores genéticos negativos en ambos sitios. Por otro lado, la procedencia de Wirrabara obtuvo siempre valores genéticos superiores al resto, mostrando ser una procedencia interesante para el programa de mejoramiento de la especie. Si los valores de heredabilidad permanecen en el tiempo, permitiría obtener importantes ganancias genéticas, y con ello mejorar significativamente la productividad de las futuras plantaciones de Eucalyptus cladocalyx.

  7. U.S. Near-Zero Emissions Program: CCS - Clean Coal R&D, FutureGen, & Demonstrations

    Energy Technology Data Exchange (ETDEWEB)

    K Der, Victor [Department of Energy (United States)

    2008-07-15

    In this paper a projection of the CO{sub 2} emissions in the United States is shown; the technical challenges in the capture and sequestration of the CO{sub 2}; what is understood by carbon sequestration; the three elements of the capture and CO{sub 2} storage that are: capture, transport, and storage; the FutureGen project; plants of coal combustion with sequestration, and at the end an initiative for the generation with clean coal is presented. [Spanish] En esta ponencia se muestra una proyeccion de las emisiones de CO{sub 2} en los Estados Unidos; los retos tecnicos en la captura y secuestro de CO{sub 2}; que entendemos por secuestro de carbono; los tres elementos de la captura y almacenamiento de CO{sub 2} que son captura, transporte y almacenamiento; el proyecto FutureGen; plantas de combustion de carbon con secuestro, y al final se presenta una iniciativa para la generacion con carbon limpio.

  8. Diversidad genética y contenido de carotenos totales en accesiones de yuca (Manihot esculenta Crantz

    Directory of Open Access Journals (Sweden)

    Ana Cruz Morillo C.

    2011-04-01

    Full Text Available La yuca (Manihot esculenta Crantz es un arbusto perenne cultivado en África, América Latina y el Sureste asiático, cuya raíz constituye una fuente importante de energía en la dieta humana en países tropicales. Los carotenoides son pigmentos naturales que se encuentran ampliamente distribuidos en la naturaleza. Se reconoce que aproximadamente cincuenta de ellos tienen actividad provitamina A, siendo b-caroteno el de mayor eficiencia para su conversión en vitamina A. El estudio de la variabilidad genética es un procedimiento útil para fortificar, enriquecer o incrementar el contenido de nutrientes de los alimentos o cultivos, entre ellos los carotenos en raíz de yuca mediante procesos de selección y recombinación en programas de mejoramiento que permitan identificar genotipos superiores. En el presente estudio, a partir de la evaluación de la diversidad genética, se generó un dendrograma de accesiones de yuca en el cual se formaron seis grupos con 68% de similitud. La heterocigosidad promedio observada fue de Ht = 0.559. Los análisis de regresión y correlación entre el contenido de carotenos totales y los datos moleculares mostraron que los marcadores que se encuentran correlacionados con altos contenidos de carotenos pertenecen al grupo de ligamiento D del mapa molecular de yuca.

  9. Distancias genéticas en poblaciones del NOA

    Directory of Open Access Journals (Sweden)

    Acreche, Noemí

    1996-01-01

    Full Text Available La mayor parte de los trabajos realizados en nuestro país sobre polimorfismos hematológicos, abordan la necesaria descripción de las poblaciones. Se pone de relieve la importancia de encarar estudios, en base a la valiosa información publicada, que vinculen los grupos con técnicas que permitan realizar nuevas inferencias sobre sus relaciones. Conocidas en gran medida en cuanto a sus manifestaciones culturales, pueden aportar desde lo genético a la comprensión de los procesos microevolutivos ocurridos en una región. Para el NOA, se ha considerado la presencia de comunidades aborígenes incluídas en cuatro familias lingüísticas. Se tendrán en cuenta estos complejos como representativos de afinidades que se establecen a partir de estrechas relaciones entre las etnias, no sólo por la lengua, sino también por las características de sus sistemas productivos, religiosidad y organización. En base a las frecuencias génicas publicadas correspondientes a los siguientes alelos: I*A, I*B, I*O; M, N, S, s; Dia , Dib; P1, P2; C, c; D, d, E, e; Le, le; Fya, Fyb; Jka, Jkb; K y k se construyeron tablas de frecuencias. Se estimaron los coeficientes de distancias genéticas que fueron analizados y posteriormente incluídos en la construcción de un fenograma de los grupos de estudio, mediante agrupaciones (Sahn Cluster secuenciales, aglomerativas, jerárquicas y anidadas. De acuerdo a la información recopilada de las frecuencias de los 25 alelos estudiados en trece poblaciones de aborígenes del NOA y Paraguay, las distancias genéticas obtenidas reflejan los caracteres lingüístico-culturales.

  10. Análisis molecular del gen TPSAB1, un gen polimórfico

    OpenAIRE

    Hernández Hernández, Laura

    2013-01-01

    [ES]Introducción: La triptasa es una serin-proteasa relacionada con la tripsina. El término triptasa comprende al menos cuatro proteasas diferentes: α, ß, γ y δ, que presentan diferencias en cuanto a sus propiedades enzimáticas. Las funciones de esta proteína no han sido completamente dilucidadas, aunque se ha implicado en fenómenos inflamatorios, protección contra la infección y otros fenómenos inmunológicos. La estructura de esta proteína es tetramérica y los genes que las codifican se encu...

  11. Review of the taxonomy of the genus Arthrobacter, emendation of the genus Arthrobacter sensu lato, proposal to reclassify selected species of the genus Arthrobacter in the novel genera Glutamicibacter gen. nov., Paeniglutamicibacter gen. nov., Pseudoglutamicibacter gen. nov., Paenarthrobacter gen. nov. and Pseudarthrobacter gen. nov., and emended description of Arthrobacter roseus.

    Science.gov (United States)

    Busse, Hans-Jürgen

    2016-01-01

    In this paper, the taxonomy of the genus Arthrobacter is discussed, from its first description in 1947 to the present state. Emphasis is given to intrageneric phylogeny and chemotaxonomic characteristics, concentrating on quinone systems, peptidoglycan compositions and polar lipid profiles. Internal groups within the genus Arthrobacter indicated from homogeneous chemotaxonomic traits and corresponding to phylogenetic grouping and/or high 16S rRNA gene sequence similarities are highlighted. Furthermore, polar lipid profiles and quinone systems of selected species are shown, filling some gaps concerning these chemotaxonomic traits. Based on phylogenetic groupings, 16S rRNA gene sequence similarities and homogeneity in peptidoglycan types, quinone systems and polar lipid profiles, a description of the genus Arthrobacter sensu lato and an emended description of Arthrobacter roseus are provided. Furthermore, reclassifications of selected species of the genus Arthrobacter into novel genera are proposed, namely Glutamicibacter gen. nov. (nine species), Paeniglutamicibacter gen. nov. (six species), Pseudoglutamicibacter gen. nov. (two species), Paenarthrobacter gen. nov. (six species) and Pseudarthrobacter gen. nov. (ten species). PMID:26486726

  12. Bioinformática y genómica funcional

    OpenAIRE

    Pérez Garrido, Isabel María

    2014-01-01

    En los últimos 20 años la Bioinformática se ha convertido en una herramienta imprescindible para el desarrollo de la genómica y la proteómica. Dada la gran afluencia de datos que se consignan diariamente en las bases de datos publicas disponibles, el conocimiento de su manejo, extracción de datos concretos así como la construcción de nuevos algoritmos que los permitan relacionar para poder formular conclusiones en relación a distintos ámbitos, como por ejemplo evolución molecular, hace que se...

  13. Cleaning the GenBank Arabidopsis thaliana data set

    DEFF Research Database (Denmark)

    Korning, Peter G.; Hebsgaard, Stefan M.; Rouze, Pierre; Brunak, Søren

    1996-01-01

    Data driven computational biology relies on the large quantities of genomic data stored in international sequence data banks. However, the possibilities are drastically impaired if the stored data is unreliable. During a project aiming to predict splice sites in the dicot Arabidopsis thaliana, we...... extracted a data set from the A. thaliana entries in GenBank. A number of simple `sanity' checks, based on the nature of the data, revealed an alarmingly high error rate. More than 15% of the most important entries extracted did contain erroneous information. In addition, a number of entries had directly...

  14. Marcadores genéticos en equinos: sus aplicaciones

    OpenAIRE

    Peral García, Pilar; Kienast, Mariana E.; Gortari, María Cecilia; Díaz, Silvana; Maderna, Carlos R.; Dulout, Fernando

    1995-01-01

    Este trabajo resume las aplicaciones básicas de los marcadores genéticos sanguíneos (MG) como herramienta útil para identificación individual y control de filiación para garantizar la fidelidad de los documentos genealógicos a nivel internacional. Además, se describe tanto la relación MG y caracteres de producción como entre MG y diferentes patologías. Finalmente, se menciona la aplicación de los MG en la prevención de la anemia hemolítica del potrillo.

  15. Homeostasis del peso corporal: Factores genéticos

    OpenAIRE

    A Marti

    2007-01-01

    Se habla del componente hereditario de la regulación del peso corporal. Los datos de los que se dispone en la actualidad sugieren que entre el 40-70% de la variación en los fenotipos de ganancia de peso/obesidad está mediada genéticamente. El número de genes, marcadores y regiones cromosómicas que han sido relacionadas con fenotipos de obesidad en humanos continúa aumentando y en la actualidad ya se han descrito más de 600.

  16. Cleaning the GenBank Arabidopsis thaliana data set

    DEFF Research Database (Denmark)

    Korning, Peter G.; Hebsgaard, Stefan M.; Rouze, Pierre; Brunak, Søren

    1996-01-01

    extracted a data set from the A. thaliana entries in GenBank. A number of simple `sanity' checks, based on the nature of the data, revealed an alarmingly high error rate. More than 15% of the most important entries extracted did contain erroneous information. In addition, a number of entries had directly......Data driven computational biology relies on the large quantities of genomic data stored in international sequence data banks. However, the possibilities are drastically impaired if the stored data is unreliable. During a project aiming to predict splice sites in the dicot Arabidopsis thaliana, we...

  17. La determinaci??n gen??tica del comportamiento humano

    OpenAIRE

    Moreno Mu??oz, Miguel

    1995-01-01

    En este trabajo se abordan varios aspectos controvertidos ligados a los avances de la biolog??a molecular de comienzos de los noventa y a numerosas aplicaciones de las t??cnicas de an??lisis y modificaci??n del material gen??tico. En particular, la oposici??n entre herencia y ambiente en aspectos de la conducta humana que se hab??an considerado muy influidos por procesos deterministas asociados con genes individuales. Aunque en una versi??n resumida, el trabajo es el resultado de un amplio re...

  18. Genética do autismo Genetics of autism

    OpenAIRE

    Gianna Carvalheira; Naja Vergani; Décio Brunoni

    2004-01-01

    O autismo é uma doença neuropsiquiátrica com profundas conseqüências sociofamilares. Inúmeros trabalhos investigaram pacientes e famílias com metodologia genético-clínica, citogenética e biologia molecular. Os resultados destes trabalhos apontam para um modelo multiloci com interação epistática associado à etiologia do autismo.Autism is a neuropsychiatric disorder with profound family and social consequences. An extraordinary number of genetical-clinical, cytogenetics and molecular studies we...

  19. TrayGen: Arranging objects for exhibition and packaging

    KAUST Repository

    Yang, Yongliang

    2013-10-01

    We present a framework, called TrayGen, to generate tray designs for the exhibition and packaging of a collection of objects. Based on principles from shape perception and visual merchandising, we abstract a number of design guidelines on how to organize the objects on the tray for the exhibition of their individual features and mutual relationships. Our framework realizes these guidelines by analyzing geometric shapes of the objects and optimizing their arrangement. We demonstrate that the resultant tray designs not only save space, but also highlight the characteristic of each object and the inter-relations between objects. © 2013 The Eurographics Association and John Wiley & Sons Ltd.

  20. Interação genótipo-ambiente em cruzamentos de bovinos de corte Genotype by environment interaction on crossbreed beef cattle

    Directory of Open Access Journals (Sweden)

    Rodrigo de Almeida Teixeira

    2006-08-01

    Full Text Available Dados de rebanhos bovinos comerciais foram analisados com o objetivo de estimar as interações dos efeitos genéticos com o ambiente que podem influenciar a avaliação de características de crescimento em rebanhos de animais puros e cruzados. O conjunto de dados analisado foi obtido a partir de animais das raças Hereford, Nelore e seus cruzamentos. As características em estudo foram os pesos à desmama e ao sobreano dos animais. As análises estatísticas foram realizadas pelo método dos quadrados mínimos e o modelo proposto incluiu os efeitos de região, grupo de contemporâneos dentro de região, mês de nascimento e sexo do bezerro, os efeitos lineares e quadráticos para a idade do bezerro e idade da vaca ao parto, ambas analisadas dentro de sexo, e os efeitos de grupo genético e da interação grupo genético × região. De modo geral, o desempenho de todos os grupos genéticos foi influenciado pelo efeito de região. Além disso, observou-se tendência de que o aumento da proporção de genes zebuínos promoveu diminuição na diferença de desempenho entre as regiões. Todos os genótipos foram beneficiados no ambiente menos restritivo, o que indica a existência de interação genótipo-ambiente e comprova a importância de que sistemas de cruzamento sejam realizados de forma a manter a adaptação das matrizes e de seus produtos.Data from Hereford and Nelore breeds and their crosses were used to estimate the effect of genotype and environment interaction on weaning and yearling weights. The statistical analyses were performed by the least-squares method using models that included region, contemporary group within region, month of birth and sex of the calf, linear and quadratic effects of age of the calf and age of the cow, both nested within sex, and the genetic group and genetic group × region interaction effects. In general, genetic groups were influenced by the region effect. Differences in performance between regions

  1. Proposal to reclassify Brenneria quercina (Hildebrand and Schroth 1967) Hauben et al. 1999 into a new genus, Lonsdalea gen. nov., as Lonsdalea quercina comb. nov., descriptions of Lonsdalea quercina subsp. quercina comb. nov., Lonsdalea quercina subsp. iberica subsp. nov. and Lonsdalea quercina subsp. britannica subsp. nov., emendation of the description of the genus Brenneria, reclassification of Dickeya dieffenbachiae as Dickeya dadantii subsp. dieffenbachiae comb. nov., and emendation of the description of Dickeya dadantii.

    Science.gov (United States)

    Brady, Carrie L; Cleenwerck, Ilse; Denman, Sandra; Venter, Stephanus N; Rodríguez-Palenzuela, Pablo; Coutinho, Teresa A; De Vos, Paul

    2012-07-01

    Bacterial isolates from oak trees in Spain and Britain, showing symptoms of bark canker and Acute Oak Decline (AOD), respectively, were examined by a polyphasic approach. Both 16S rRNA gene sequencing and multilocus sequence analysis (MLSA), based on partial sequences of gyrB, rpoB, infB and atpD genes, revealed that the isolates were separated into two genetic groups according to their origin. Their closest phylogenetic relative was Brenneria quercina, the causal agent of drippy nut disease of oak, which clustered distant to the other species of the genus Brenneria. MLSA data for species of the genera Brenneria, Pectobacterium, Dickeya, Erwinia, Pantoea and Samsonia confirmed the polyphyletic nature of the genus Brenneria and indicated synonymy of Dickeya dadantii and Dickeya dieffenbachiae. DNA-DNA hybridization experiments confirmed this synonymy and also revealed DNA-DNA relatedness values of 58-73% between the new oak isolates and B. quercina. Phenotypic and/or chemotaxonomic methods allowed B. quercina and the two genetic groups of new oak isolates to be discriminated from other recognized species of the genus Brenneria and from members of the closely related genera Dickeya, Pectobacterium and Samsonia. Based on the data obtained, the following taxonomic proposals are made: (1) reclassification of B. quercina as the type species of a novel genus, Lonsdalea gen. nov., as Lonsdalea quercina comb. nov. (type strain LMG 2724(T)=ATCC 29281(T)=CCUG 48867(T)=CFBP 3617(T)=CIP 105201(T)=DSM 4561(T)=ICMP 1845(T)), (2) classification of the oak isolates as Lonsdalea quercina subsp. iberica subsp. nov. (type strain LMG26264(T)=NCPPB 4490(T)) and Lonsdalea quercina subsp. britannica subsp. nov. (type strain LMG 26267(T)=NCPPB 4481(T)) and leading to the automatic creation of Lonsdalea quercina subsp. quercina subsp. nov. (type strain LMG 2724(T)=ATCC 29281(T)), (3) emendation of the description of the genus Brenneria, and (4) reclassification of Dickeya dieffenbachiae as

  2. Caracterización genética de restos antiguos de poblaciones indígenas de Colombia, mediante diez loci con repeticiones cortas en tandem (STR's

    Directory of Open Access Journals (Sweden)

    Alape J.

    2001-06-01

    Full Text Available En nuestros días interactúan lo ancestral y lo moderno, la eliminación y la conservación, lo antro-pológico y lo molecular, la acumulación de paradigmas o la postulación de hipótesis y el quehacercientífico; estamos en un momento de grandes y aceleradas transformaciones y búsquedas de locultural, lo genético y por qué no decirlo, lo social y económico. Todo ello incide significati-vamente en el medio natural y encontramos que al plantearse una diversidad genética, aparececierto tipo de preguntas encaminadas a dilucidar los procesos y transformaciones evolutivas quehan dado origen y distribución a las diferentes especies remanentes hoy sobre la tierra. Para darrespuesta a todo esto, es necesario implementar metodologías como los estudios de genéticamolecular con individuos vivos o recurriendo a los restos óseos o de tejidos que aún se conservan.

  3. Diversidade genética em quiabeiro baseada em marcadores RAPD Genetic diversity in okra using RAPD markers

    Directory of Open Access Journals (Sweden)

    Gilmar E. Martinello

    2003-03-01

    Full Text Available Avaliou-se a utilização de marcadores RAPD para estimar a diversidade em 42 acessos do gênero Abelmoschus e um de Hibiscus. As estimativas das distâncias genéticas foram feitas com base no complemento aritmético do Índice de Jaccard. Foram utilizadas as técnicas de análise multivariada, através de agrupamento hierárquico do vizinho mais próximo e método de Tocher, para estudar os arranjos dos grupos de genótipos, bem como analisar os métodos de agrupamentos empregados. Trinta e um iniciadores foram utilizados para amplificar fragmentos de DNA pela reação de polimerização em cadeia (PCR e foram gerados 103 fragmentos RAPD. O agrupamento hierárquico dos 43 genótipos com base no método do vizinho mais próximo separou os acessos, de modo geral, conforme as espécies botânicas, formando 6 grupos distintos. Isto foi confirmado pela projeção das distâncias genéticas no plano bidimensional, onde o primeiro e maior dos grupos reuniu os acessos de A. esculentus e A. caillei. Por outro lado, o método de Tocher reuniu 90% do germoplasma no grupo I incluindo, neste, os acessos de A. moschatus e A. manihot, além das outras duas espécies anteriores. O método de otimização de Tocher permitiu a formação de apenas 4 grupos de genótipos, mostrando-se coerente apenas em parte à análise de agrupamento hierárquico. Porém, o reagrupamento dos acessos do grupo I de Tocher pelo método hierárquico, revelou a existência de maior heterogeneidade genética no germoplasma estudado.RAPD markers were utilized to estimate the diversity among 42 Abelmoschus and 1 Hibiscus accessions . The genetic distances were based on the arithmetic complement of the Jaccard index. For this purpose we used the multivariate analysis technique by hierarchycal single linkage and the Tocher methods to obtain the genotypes agglomeration as well as to analyze the methods employed. Thirty-one random decamer primers were used do amplify DNA by the polymerase

  4. Genética de poplaciones de Atta sexdens rubropilosa (Hymenoptera: Formicidae POPULATION GENETICS OF Atta sexdens rubropilosa (HYMENOPTERA: FORMICIDAE

    Directory of Open Access Journals (Sweden)

    LIRIANA BELIZÁRIO CANTAGALLI

    2013-04-01

    Full Text Available La variabilidad genética de las hormigas Atta sexdens rubropilosa colectadas en cinco lugares distintos de Brasil fueron evaluados por la técnica PCR-RAPD. Un total de 15 primers produjeron 148 fragmentos, de los cuales 123 fueron polimórficos, lo que corresponden al 83,11 %. La estimación de la diversidad genética por el índice de Shannon fue 0,3836 y el desviación estándar fue de ± 0,2335. Estos valores demuestran una alta diversidad genética. El valor de G ST fue 0,2372 y ΦPT = 0,184 lo que indica que las poblaciones están moderadamente diferenciadas y que el 82 % de la variación obtenida se produce dentro de las poblaciones. Aunque la prueba de Mantel ha demostrado una correlación entre la distancia genética y geográfica se observó que Ivatuba e Itambé (33,8 km tiene una pequeña distancia geográfica y la mayor distancia genética. La distancia inferior genética fue estimada para Maringá e Ivatuba pero estas localidades cuentan con una distancia geográfica pequeña (42,3 km, lo que indica que no hay barreras para el apareamiento entre los reproductores en estas poblaciones. El alto valor de polimorfismo (83,11 % y la capacidad de emparejamiento entre las poblaciones presentes en las regiones estudiadas, indican que esta especie de hormiga cortadora está bien adaptada a la región, y deben ser desarrollados programas integrados de control si se convierten en plagas.The genetic variability of Atta sexdens rubropilosa leaf-cutting ants collected from five brazilian localities was evaluated with PCR-RAPD technique. We used 15 primers producing 148 fragments of which 123 (83.11 % contained polymorphisms. The estimated Shannon index was 0.3836 ± 0.2335 showing that these ants possess high genetic diversity. The G ST value was 0.2372 and ΦPT = 0.184, indicating that the analyzed populations are moderately differentiated and 82 % of the variation obtained occur within populations. Although Mantel's test had shown correlation

  5. Métodos de análisis genéticos, espaciales y de conectividad en genética del paisaje

    OpenAIRE

    Tania Garrido-Garduño; Ella Vázquez-Domínguez

    2013-01-01

    La genética del paisaje tiene como objetivo evaluar la interacción de características del paisaje (es decir, montañas, corredores) y variables ambientales como temperatura o humedad con procesos microevolutivos como flujo genético, deriva génica o selección. Para ello, la genética del paisaje se apoya en métodos de análisis de otras disciplinas como genética de poblaciones, ecología del paisaje y estadística espacial, y cuenta además con métodos propios. En este trabajo explicamos qué es la g...

  6. Update History of This Database - GenLibi | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us GenLibi Update History...base Database Description Download License Update History of This Database Site P...olicy | Contact Us Update History of This Database - GenLibi | LSDB Archive ...

  7. A 48-plex autosomal SNP GenPlex™ assay for human individualization and relationship testing

    DEFF Research Database (Denmark)

    Tomas Mas, Carmen; Børsting, Claus; Morling, Niels

    2012-01-01

    SNPs are being increasingly used by forensic laboratories. Different platforms have been developed for SNP typing. We describe the GenPlex™ HID system protocol, a new SNP-typing platform developed by Applied Biosystems where 48 of the 52 SNPforID SNPs and amelogenin are included. The GenPlex™ HID...

  8. CottonGen: a genomics, genetics and breeding database for cotton research

    Science.gov (United States)

    CottonGen (http://www.cottongen.org) is a curated and integrated web-based relational database providing access to publicly available genomic, genetic and breeding data for cotton. CottonGen supercedes CottonDB and the Cotton Marker Database, with enhanced tools for easier data sharing, mining, vis...

  9. Pseudolimia n. gen., a new monotypic genus for Limia heterandria Regan, 1913 (Teleostei: Poeciliidae)

    NARCIS (Netherlands)

    Poeser, Fred N.

    2002-01-01

    A new genus, Pseudolimia n. gen. is established for the South American Limia heterandria Regan, 1913. It is compared in an anatomical analysis with several nominal taxa of the tribe Poeciliini. Based on number and shape of the gonapophyses, Pseudolimia n. gen. is assigned to the tribe Poeciliini.

  10. Interação genótipo x ambiente em genótipos de feijão-caupi semiprostrado via modelos mistos

    Directory of Open Access Journals (Sweden)

    Francisco Eduardo Torres

    2015-01-01

    Full Text Available A metodologia de modelos mistos (REML/BLUP tem sido empregada para estudar os efeitos da interação genótipo x ambiente (G x E em várias culturas, como: arroz, feijão, cana-de-açúcar, cajueiro e eucalipto, porém ainda não foi aplicada em feijão-caupi. Assim, o objetivo deste trabalho foi selecionar simultaneamente genótipos de feijão-caupi semiprostrado cultivados no Estado do Mato Grosso do Sul, via modelos mistos, que reúnam alta adaptabilidade, estabilidade e produtividade de grãos. Foram conduzidos quatro ensaios de valor de cultivo e uso com genótipos de genótipos de feijão-caupi nos anos de 2005 e 2006 em Aquidauana, Chapadão do Sul e Dourados. O delineamento experimental utilizado foi o de blocos completos casualizados, com 20 genótipos e 4 repetições. Os parâmetros genéticos foram estimados pela metodologia REML/BLUP, e a seleção baseou-se no método da média harmônica do desempenho relativo dos valores genéticos (MHPRVG, em três estratégias: seleção com base no valor genético predito, tendo-se considerado o desempenho médio dos genótipos em todos os ambientes (sem efeito de interação ou o desempenho em cada ambiente (com efeito da interação; e seleção simultânea quanto à produtividade de grãos, estabilidade e adaptabilidade. Os genótipos BRS Paraguaçu, MNC99-542F-5 e MNC99-508G-1 podem ser cultivados em vários ambientes, pois reúnem alta produtividade de grãos, adaptabilidade e estabilidade. A herdabilidade da média dos genótipos apresentou magnitude variando de moderada a alta, fato que indica excelentes possibilidades para a seleção, permitindo acurácia seletiva de 82%.

  11. Divergence and genetic variability among superior rubber tree genotypes Divergência e variabilidade genética de genótipos superiores de seringueira

    Directory of Open Access Journals (Sweden)

    Lígia Regina Lima Gouvêa

    2010-02-01

    Full Text Available The objective of this work was to estimate the genetic variability and divergence among 22 superior rubber tree (Hevea sp. genotypes of the IAC 400 series. Univariate and multivariate analyses were performed using eight quantitative traits (descriptors, including yield. In the univariate analyses, the estimated parameters were: genetic and environmental variances; genetic and environmental coefficients of variation; and the variation index. The Mahalanobis generalized distance, the Tocher agglomerative method and canonical variables were used for the multivariate analyses. In the univariate analyses, variability was verified among the genotypes for all the variables evaluated. The Tocher method grouped the genotypes into 11 clusters of dissimilarity. The first four canonical variables explained 87.93% of the cumulative variation. The highest genetic variability was found in rubber yield-related traits, which contributed the most to the genetic divergence. The most divergent pairs of genotypes are suggested for crossbreeding. The genotypes evaluated are suitable for breeding and may be used to continue the IAC rubber tree breeding program.O objetivo deste trabalho foi estimar a divergência e a variabilidade genética entre 22 genótipos superiores de seringueira (Hevea sp. da série IAC 400. Análises univariadas e multivariadas foram realizadas com oito caracteres quantitativos (descritores, incluindo produtividade. Na análise univariada, os parâmetros estimados foram: variâncias genética e ambiental, coeficientes de variação genética e ambiental, e índice de variação. A distância generalizada de Mahalanobis, o método aglomerativo de Tocher e variáveis canônicas foram utilizados nas análises multivariadas. Nas análises univariadas, verificou-se variabilidade entre os genótipos para todas as variáveis avaliadas. O método de Tocher agrupou os genótipos em 11 grupos de dissimilaridade. As quatro primeiras variáveis can

  12. Moureanthidium, gen.n. de Dianthidiini do Brasil (Hymenoptera, Megachilidae Moureanthidium, gen.n. de Dianthidiini from Brazil (Hymenoptera, Megachiliiae

    Directory of Open Access Journals (Sweden)

    Danúncia Urban

    1995-01-01

    Full Text Available Moureanthidium, gen.n. is proposed for Dianthidium subarenarium Schwarz, 1933 and to include four new species of Anthidiine bees from Brazil, Moureanthidium paranaense, sp.n from Ponta Grossa, Paraná, Moureanthidium capixaba, sp.n. from Santa Teresa, Espírito Santo, Moureanthidium bahianum, sp.n. from Vitória da Conquista, Bahia and Moureanthidium catarinense, sp.n. from Seara, Santa Catarina. The males are characterized by the border of the seventh tergum strongly reflexed, densely hairy and with a pair of lateral projections and the third sternite with two spines or little projections at the middle, near the apex.

  13. Ambiente genético del gen blactx-m-12 en aislamientos hospitalarios de klebsiella pneumoniae

    OpenAIRE

    Celis Bustos, Yamile Adriana; Pulido Manrique, Ingrid Yamile; Valenzuela de Silva, Emilia María; Reguero Reza, María Teresa; Mantilla Anaya, José Ramón

    2009-01-01

    The blaCTX-M-12 gene’s genetic environmnt in Klebsiella pneumoniae hospital isolates Resumen: En Colombia se han detectado genes del grupo CTX-M-1 con alta frecuencia en aislamientos de Klebsiella pneumoniae causantes de infección intrahospitalaria. El conocimiento de los factores genéticos que pueden favorecer la diseminación de estos genes entre especies bacterianas es un aspecto importante para el control de la resistencia. En este estudio se identificaron los plásmidos portadores del ge...

  14. Ambiente genético del Gen blaCTX-M-12 en aislamientos hospitalarios de Klebsiella pneumoniae

    OpenAIRE

    Yamile Adriana Celis Bustos; Ingrid Yamile Pulido Manrique; Emilia María Valenzuela de Silva; María Teresa Reguero Reza; José Ramón Mantilla Anaya

    2009-01-01

    The blaCTX-M-12 gene’s genetic environmnt in Klebsiella pneumoniae hospital isolates Resumen: En Colombia se han detectado genes del grupo CTX-M-1 con alta frecuencia en aislamientos de Klebsiella pneumoniae causantes de infección intrahospitalaria. El conocimiento de los factores genéticos que pueden favorecer la diseminación de estos genes entre especies bacterianas es un aspecto importante para el control de la resistencia. En este estudio se identificaron los plásmidos portadores del ge...

  15. Next gen wavelets down-sampling preserving statistics

    Science.gov (United States)

    Szu, Harold; Miao, Lidan; Chanyagon, Pornchai; Cader, Masud

    2007-04-01

    We extend the 2 nd Gen Discrete Wavelet Transform (DWT) of Swelden to the Next Generations (NG) Digital Wavelet Transform (DWT) preserving the statistical salient features. The lossless NG DWT accomplishes the data compression of "wellness baseline profiles (WBP)" of aging population at homes. For medical monitoring system at home fronts we translate the military experience to dual usage of veterans & civilian alike with the following three requirements: (i) Data Compression: The necessary down sampling reduces the immense amount of data of individual WBP from hours to days and to weeks for primary caretakers in terms of moments, e.g. mean value, variance, etc., without the artifacts caused by FFT arbitrary windowing. (ii) Lossless: our new NG_DWT must preserve the original data sets. (iii) Phase Transition: NG_DWT must capture the critical phase transition of the wellness toward the sickness with simultaneous display of local statistical moments. According to the Nyquist sampling theory, assuming a band-limited wellness physiology, we must sample the WBP at least twice per day since it is changing diurnally and seasonally. Since NG_DWT, like the 2 nd Gen, is lossless, we can reconstruct the original time series for the physicians' second looks. This technique of NG_DWT can also help stock market day-traders monitoring the volatility of multiple portfolios without artificial horizon artifacts.

  16. Development of Basic Key Technologies for Gen IV SFR Systems

    International Nuclear Information System (INIS)

    In this project, research and developments have been done to verify the applicability of advanced design features of Gen IV SFR system. Through the evaluation of various design concept, fluid system of Gen IV SFR was established which is enhanced in safety and economics. Especially, passive decay heat removal system PDRC was developed with high reliability through enough capacity and diversity. For the development of advanced technologies, basic design of experimental facility were established in order to conserve overall and local thermal hydraulic phenomena of PDRC and major experiment requirements were established. In order to develop super-critical CO2 Brayton Cycle system, design concept of new airfoil type heat exchange was developed and through an experimental test, performance of the airfoil type compact heat exchanger was evaluated. To develop performance analysis code, an NSSS analysis model and related control systems were developed, a model for a supercritical CO2 Brayton Cycle was implemented. A Rankine cycle model including control systems was developed

  17. Parámetros genéticos del crecimiento y la reproducción en ganado cebú

    Directory of Open Access Journals (Sweden)

    Mario Pino Pérez de Utrera

    2009-06-01

    Full Text Available Con el propósito de conocer las correlaciones genéticas entre los rasgos de crecimiento en toros y las características reproductivas en vacas cebú de Cuba, se utilizaron 9490 registros de toros que terminaron la prueba de comportamiento en pastoreo, a los cuales se les registró el peso final (PF a los 18 meses de edad; así mismo se emplearon 13.575 registros de sus medio hermanas, a las que se les registró la edad a la primera inseminación (EPI, la edad al primer parto (EPP y los servicios por gestación (SXG. Los datos fueron colectados en cinco centros genéticos entre 1988 y 2001. Todos los animales eran hijos de 267 toros. Los valores promedios encontrados fueron 343,3 kg, 26,5 meses, 37,7 meses y 1,76 para PF, EPI, EPP y SXG, respectivamente. Los índices de herencia (h2 fueron 0,245, 0,054, 0,091 y 0,021 para PF, EPI, EPP y SXG, respectivamente. Las correlaciones genéticas entre PF y los rasgos reproductivos oscilaron entre -0,216 y -0,340. Entre los caracteres reproductivos, las correlaciones genéticas fueron de 0,544 a 0,982. Se concluyó que los valores de h2 estimados para el ganado cebú en Cuba están dentro del rango publicado en la literatura internacional y que la selección continuada para el incremento del peso corporal a los 18 meses de edad en los toros no afectaría negativamente la eficiencia reproductiva en las vacas.

  18. Pautas éticas para la investigación genética en Antropología Biológica

    Directory of Open Access Journals (Sweden)

    Caratini, Alicia Liliana

    2007-01-01

    Full Text Available El abordaje de la ética de la investigación en el área de la genética de poblaciones es de orden colectivo en un contexto de multiculturalidad, haciendose imprescindible el respeto a las pautas culturales y a las formas de organización social de los grupos involucrados: poblaciones originarias y/o cosmopolitas. Lo importante es que los acuerdos alcanzados cobijen sin discriminación a cada uno de los actores que desarrollan el proceso de investigación tanto a los investigadores como a las poblaciones–sujetos. Todas las pautas éticas se hallan enmarcadas por los principios que fijan los convenios Internacionales como el Código de Nuremberg (1947, Declaración de Helsinki (1964 y las sucesivas revisiones, las normas internacionales de CIOMS (1991-1993 la Declaración internacional sobre los datos genéticos Humanos, UNESCO (2003, y las legislaciones regionales y locales. Los procedimientos deben cumplir sintéticamente los siguientes pasos: 1 Recolección de los datos genéticos, se debe tener el consentimiento, previo, libre e informado de las poblaciones-sujetos; 2 confidencialidad de los datos identitarios; 3 el derecho a ser informado, las poblaciones involucradas deben tener acceso a los resultados de la investigación; 4 se debe observar en la cooperación e intercambio internacional el respeto mutuo de acuerdo al art.18 de la Declaración sobre los datos genéticos Humanos (UNESCO 2003. El cumplimiento de las pautas éticas debe estar supervisado por Comités de Etica interdisciplinarios en los organismos de financiamiento de los proyectos.

  19. Interacciones genéticas y epigenéticas relacionadas con fisuras de labio y paladar no sindrómicas

    Directory of Open Access Journals (Sweden)

    L.R. Tirado Amador

    2016-02-01

    Full Text Available En la actualidad, las fisuras de labio y fisuras de paladar no sindrómicas constituyen una de las alteraciones del crecimiento y desarrollo facial con alta prevalencia que ocasiona preocupación a nivel mundial porque generan efectos a nivel fisiológico al repercutir en la deglución, fonación, así como en la autopercepción y aceptación del individuo en lo relacionado con la socialización, ocasionando que el tratamiento de dicha alteración requiera de la utilización de un equipo interdisciplinario con el fin de lograr mejoría satisfaciendo las necesidades del individuo afectado. Con respecto a la etiología de este defecto del desarrollo, en la literatura se ha atribuido a diversos factores genéticos así como a factores ambientales, convergiendo hacia el modelo de interacción multifactorial en el que los genes emiten respuesta ante los estímulos que provienen del ambiente, lo que repercute en la expresión a nivel fenotípico. Aunque existen reportes de los cambios genéticos que pueden estar asociados con las alteraciones en el desarrollo labial y cierre palatino, existe gran tendencia a considerar la presencia de mutaciones; sin embargo en ocasiones se omite o resta cierta importancia a los efectos que derivan de las alteraciones en la expresión de la información genética como resultado de la exposición a ciertos factores ambientales. Por lo cual, en la presente revisión, los autores pretenden describir algunos factores genéticos y epigenéticos que intervienen en la aparición de las fisuras labiales y palatinas no sindrómicas.

  20. Identificación genética de subpoblaciones de aedes aegypti en Cartagena de Indias – Colombia

    Directory of Open Access Journals (Sweden)

    Malambo Dacia

    2011-09-01

    Full Text Available INTRODUCCIÓN: el mosquito Aedes aegypti es el principal vector de la fiebre Dengue (FD en ambientes urbanos y suburbanos enpaíses tropicales y subtropicales. Se estima que la FD infecta 50 millones de personas anualmente y más de quinientas milcontraen su forma más grave (PAHO 2008; Romano, 2010. En Colombia se notificaron 127.292 casos, 8.919 de dengue grave y 138muertes. El estudio de la variabilidad genética de una población de Ae. Aegypti permite identificar sub-poblaciones a través delespacio geográfico. Tal variación genética se ha relacionado con una expresión heterogénea en la capacidad vectorial del insectofrente a la FD debido a procesos de selección sobre serotipos virales circulantes aun a escala local.OBJETIVO: describir la variabilidad genética de Ae. Aegypti en Cartagena de Indias.MÉTODOS: estudio de corte transversal mediante un muestreo aleatorio por conglomerados que incluyó las 15 comunasadministrativas del distrito. 6300 viviendas fueron inspeccionadas mediante el protocolo de vigilancia entomológica dirigido alarvas de Ae. Aegypti. La implementación de las pruebas moleculares se realizó sobre 260 insectos de 36 sitios geográficosseleccionados al azar. La extracción del DNA genómico fue realizado mediante el método CTAB. La prueba RAPD PCR empleó losoligonucleótidos iniciadores: TTGCACCAACAGTCAATGTCTTCAGGTTC, CGTCTCAGTGATCCGGGGG y CGCCACAAGGGCCATGAACAG. Se realizó una electroforesis a los productos de amplificación RAPDs en un gel de agarosa al 1%, el cual fue conbromuro de etídio y fotodocumentado bajo luz UV. Las bandas obtenidas fueron asimiladas en una matriz binaria. Fueronutilizadas 1000 réplicas para obtener una matriz de distancia utilizando el paquete PHYLIP. El análisis filogenéticos se realizómediante el método de Neighbor-Joining y la visualización del árbol de consenso mediante el programa Tree view.RESULTADOS: 1361 viviendas fueron positivas a larvas del mosquito. Los perfiles