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Sample records for al complejo mycobacterium

  1. Susceptibilidad in vitro a los medicamentos anti-tuberculosos de aislados de cepas del complejo Mycobacterium tuberculosis obtenidos a partir de lobos marinos

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    Amelia Bernardelli

    2004-06-01

    Full Text Available Se han hallado cepas de micobacterias aisladas de lobos marinos del Atlántico sur y pertenecen al complejo de Mycobacterium tuberculosis. Los animales se recibieron en las instalaciones del Oceanario Mundo Marino y fueron tratados apropiadamente para su recuperación con la terapia convencional, cuidados intensivos y suplemento alimentario pero no se observó mejoría en su estado general. Se practicaron necropsias en todos los animales y se observaron lesiones extensas compatibles con tuberculosis en pulmones, hígado, bazo y ganglios linfáticos. Para la identificación de las micobacterias, se realizaron pruebas bioquímicas y técnicas de biología molecular con la sonda IS6110. Además, se identificaron todas las cepas como pertenecientes al complejo M. tuberculosis mediante el equipo LCx M. tuberculosis Assay (Abbott Laboratories. El objetivo de este estudio fue determinar in vitro la sensibilidad de las cepas patrón BCG, H37Rv (M. tuberculosis y AN5 (Mycobacterium bovis y la de las siete aisladas de lobos marinos a isoniacida, rifampicina, estreptomicina y etambutol. La concentración inhibitoria mínima (CIM de las drogas antituberculosas se llevó a cabo con el equipo Mycobacterial Growth Indicator Tube (MGIT, BD, Argentina y la microdilución con el ensayo colorimétrico con bromuro de 3-(4-5 dimetiltiazol-2-2,5 difeniltetrazolio. Todos los aislamientos y las cepas de referencia BCG y AN5 se inhibieron con valores CIM de los de H37Rv con buena concordancia entre los resultados obtenidos con ambas técnicas. Los hallazgos permiten sugerir que podrían ser una importante ayuda terapéutica en los lobos marinos con diagnóstico de tuberculosis y evaluar el posible papel sanitario en la prevención y transmisión de la tuberculosis de los animales a los humanos y el trabajo en conjunto.

  2. Estudio de la adición de estabilizantes y vitamina B12 al complejo inyectable de hierro dextrana

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    Georgina Michelena; Antonio Bell; Graciela Cerutti; Emilia Carrera; Mónica Coronel; Gracia Rivas Monte

    2007-01-01

    Se realizó el estudio de adición de estabilizantes al complejo hierro dextrana mediante un diseño experimental 33 tipo mezcla con el objetivo de aumentar la fijación del hierro en el proceso de síntesis y disminuir su viscosidad, lo que incide favorablemente en la tecnología al obtener un producto de mayor filtrabilidad y con alto contenido de hierro. Los mejores resultados se alcanzaron con la variante de adición en iguales relaciones de ácido cítrico y sorbitol y tres veces la cantidad en d...

  3. ESTUDIO TERMODINÁMICO Y CINÉTICO DE LA FORMACIÓN DEL COMPLEJO 2' (OHACETOFENONA-Al(III

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    Yamina A. Dávila

    2011-01-01

    Full Text Available En este trabajo se realizó un estudio experimental basado en la velocidad de formación, estabilidad y estructura del complejo formado entre el Al(III y 2 ́(OHacetofenona en metanol y etanol usando espectroscopía UV- visible. Fueron estudiadas las influencias de la temperatura (15-35°C, fuerza iónica (1,7x10-3 – 7,8x10-3 M y permitividad relativa del solvente (ε r = 24,55 y 32,63 sobre la velocidad de reacción. Aplicando el método de la relación molar, se determinó la formación de un complejo de estequiometría 1:1. Además, fue calculada la constante de formación del complejo en metanol (KC= 6,08x105. Para la reacción de complejación (usando la Teoría del estado de transición los valores obtenidos fueron: MeOH ΔH‡ 18,7 kcal/mol y ΔS‡ 9,75 cal/K mol, y para EtOH ΔH‡ 23,0 kcal/mol y ΔS‡ 24,9 cal/K mol. Estas experiencias muestran que la velocidad específica de reacción aumenta con el descenso de la permitividad del medio y disminuye cuando la fuerza iónica del medio es mayor.

  4. Información, señal, comunidad y contexto: su importancia al tratar con sistemas adaptativos complejos

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    Jordi Guzman

    2007-07-01

    Full Text Available El artículo trata de las diferencias entre información y conocimiento, así como de la relevancia del contexto en el aprendizaje, el cual se da dentro de una cultura en competencia por su supervivencia con otras culturas. Estas características definen un sistema adaptativo complejo, que se asemeja a la conducta de las especies que luchan por sobrevivir a los cambios de un entorno en continuo movimiento.

  5. Complejo Oficinas Cabanyal

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    CAMPOS CARCELLER, RAMÓN

    2015-01-01

    El proyecto es un complejo de oficinas con usos complementarios. Está ubicado en el barrio marítimo del Cabañal de Valencia. La zona más pública del complejo está abierta al barrio y permite el uso de sus instalaciones por parte de los vecinos. En esta planta se encuentran los usos complementarios: cafetería, restaurante, salón de actos, biblioteca, zonas de estudio en grupo, salas de exposiciones y guardería. La planta baja se divide en tres zonas: edificio de hall de las oficinas y comercio...

  6. Diez pasos para el aprendizaje complejo: Un acercamiento sistemático al diseňo instruccional de los cuatro componentes

    NARCIS (Netherlands)

    Van Merriënboer, Jeroen; Kirschner, Paul A.

    2011-01-01

    Van Merriënboer, J. J. G., & Kirschner, P. A. (2010). Diez pasos para el aprendizaje complejo: Un acercamiento sistemático al diseňo instruccional de los cuatro componentes (translation from English). Cuajimalpa, México: Aseguramiento de la Calidad en la Educacion y en el Trabajo.

  7. Soft tissue abscess and lymphadenitis due to Mycobacterium avium Complex as an expression of immune reconstitution inflammatory syndrome after a second scheme of highly active antiretroviral therapy Linfadenitis y absceso subcutáneo por Complejo Mycobacterium avium como manifestación de síndrome inflamatorio de reconstitución inmune luego de un segundo esquema de terapia antirretroviral de gran actividad

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    Marcelo Corti

    2007-08-01

    Full Text Available Immune reconstitution inflammatory syndrome (IRIS is an atypical and unexpected reaction related to highly active antiretroviral therapy (HAART in human immunodeficiency virus (HIV infected patients. IRIS includes an atypical response to an opportunistic pathogen (generally Mycobacterium tuberculosis, Mycobacterium avium complex, cytomegalovirus and herpes varicella-zoster, in patients responding to HAART with a reduction of plasma viral load and evidence of immune restoration based on increase of CD4+ T-cell count. We reported a case of a patient with AIDS which, after a first failure of HAART, developed a subcutaneous abscess and supraclavicular lymphadenitis as an expression of IRIS due to Mycobacterium avium complex after starting a second scheme of HAART.El síndrome inflamatorio de reconstitución inmune (SIRI es una reacción atípica e inesperada relacionada con el tratamiento antirretroviral de gran actividad (TARGA en pacientes infectados por el virus de la inmunodeficiencia humana (VIH. El SIRI representa una respuesta inflamatoria frente a un patógeno oportunista (generalmente Mycobacterium tuberculosis, Complejo Mycobacterium avium, citomegalovirus y herpes varicela-zóster en pacientes que responden a la TARGA con una marcada reducción de la carga viral en plasma y evidencia de una recuperación inmunológica expresada por el incremento de los niveles de linfocitos T CD4+. Presentamos el caso de un paciente con síndrome de inmunodeficiencia adquirida que desarrolló un absceso subcutáneo en muslo derecho y una adenitis supraclavicular izquierda como manifestación de SIRI por Complejo Mycobacterium avium luego del inicio de un segundo esquema de TARGA.

  8. Aspectos bioquímicos y moleculares de las deficiencias de complejo I mitocondrial ligados al cromosoma X

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    Fernández Moreira, Daniel

    2010-01-01

    El objetivo global de este trabajo ha sido investigar posibles mutaciones en los genes ligados al cromosoma X, NDUFA1 y NDUFB11, asociadas a alteraciones primarias del proceso de la fosforilación oxidativa.

  9. Dinámicas emergentes de la realidad: del Pensamiento Complejo al Pensamiento Sistémico Autopoiético

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    Wilmar Peña Collazos

    2008-01-01

    Full Text Available Las metáforas que se tejen en torno al pensamiento complejo y al pensamiento sistémico autopoiético generan distintas visiones y aprehensiones de la realidad, apreciaciones muy polémicas que merecen un análisis detallado y juicioso, pues afectan problemáticas de orden ontológico y epistemológico definitivos en el abordaje científico. La visión moriniana de pretender una conciencia planetaria que religue las voluntades parece estar muy lejana de las crisis de la realidad. El mito del desarrollo es una concepción reduccionista que olvida las principales claves de la humanidad. Para comprender la realidad humana es necesaria una verdadera afirmación sobre la naturaleza biológica, psicológica o espiritual, de lo humano. Para H. Maturana y F. Varela el conocimiento es "vida" y la representación no es simplemente una imagen del mundo, sino una reconstrucción interna del propio organismo y su sistema. El organismo está predestinado con esquemas para reconstruirlo.

  10. Adenopatía supraclavicular como forma de presentación de un carcinoma de cérvix asociado al complejo esclerosis tuberosa con linfangioleiomiomatosis

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    Pablo López Mato; Alfonso Varela Fariña; Elena Seco Hernández; Antonio J. Chamorro Fernández

    2013-01-01

    La linfangioleiomiomatosis es una proliferación del tejido muscular broncovascular que recientemente se ha definido como una expresión incompleta de la entidad “complejo esclerosis tuberosa”, una facomatosis a la que se asocian diversas neoplasias. Presentamos un caso de carcinoma de cérvix con metástasis supraclaviculares y cervicales, asociado a linfangioleiomiomatosis en el contexto de un “complejo esclerosis tuberosa”.

  11. Adenopatía supraclavicular como forma de presentación de un carcinoma de cérvix asociado al complejo esclerosis tuberosa con linfangioleiomiomatosis

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    Pablo López Mato

    2013-09-01

    Full Text Available La linfangioleiomiomatosis es una proliferación del tejido muscular broncovascular que recientemente se ha definido como una expresión incompleta de la entidad “complejo esclerosis tuberosa”, una facomatosis a la que se asocian diversas neoplasias. Presentamos un caso de carcinoma de cérvix con metástasis supraclaviculares y cervicales, asociado a linfangioleiomiomatosis en el contexto de un “complejo esclerosis tuberosa”.

  12. Aproximación al concepto de ansiedad en psicología: su carácter complejo y multidimensional

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    María Teresa GONZÁLEZ MARTÍNEZ

    2009-11-01

    Full Text Available La ansiedad, estado emotivo y respuesta que surge cuando uno se halla expuesto a situaciones que impliquen peligro o amenaza, es una experiencia universal y cotidiana para todo ser humano. Es, además, una conducta que cumple una función esencialmente adaptativa. Ahora bien, la ansiedad puede ser también una conducta patológica, cuando, por la continuidad de las respuestas o la excesiva intensidad de las mismas, no se logra establecer el equilibrio entre el sujeto y los estímulos. Estamos, pues, ante un concepto complejo en el que existen implícitos diferentes significados. A ello ha contribuido el hecho de que la ansiedad es un tema abordado desde ámbitos disciplinarios muy diferentes (filosofía, biología, psicología, sociología, entre otros.] Pero especialmente esto ha sido favorecido por la pluralidad teórica de la psicología que lleva a cada teoría a proponer un modelo explicativo de la ansiedad. A pesar de ello, los progresos actuales sobre el conocimiento de los mecanismos de la ansiedad y sus consecuencias, permiten que se haya llegado al acuerdo de un concepto de ansiedad, válido desde los distintos ámbitos disciplinarios y teóricos, como una forma de conducta compleja y multidimensional en la que existen componentes de respuesta fisiológicos, motores y subjetivo-cognitivos. Este patrón de conducta surge cuando un individuo se halla expuesto a estímulos interpretados como amenazantes, bien porque objetivamente lo sean, o porque él subjetivamente así lo vivencia.

  13. La brecha de la “complejidad”: perfil eAprendiz como propuesta de adecuación personal al nuevo entorno “vital”, expandido y complejo

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    Rubio Royo, Enrique

    2011-12-01

    ón con un nivel de interconexión e interdependencia sin precedentes (causa de su complejidad, con nuevas estructuras (redes y con nuevas tecnologías sociales, que configuran a Internet como infraestructura digital de transformación (disruptiva y, también, de adecuación. Como resultado de todo ello, un “nuevo ENTORNO vital, expandido y complejo”, impacta notablemente en la manera en que nos relacionamos, vivimos, trabajamos y aprendemos. Nos encontramos, pues, ante una nueva clase de situaciones o problemas (complejos que, a la hora de enfrentarnos a los mismos, nos requiere no solo cambiar nuestra forma de pensar y percibir la realidad (pensamiento sistémico, sino también la adopción de una nueva cultura digital, de interdependencia y sostenibilidad. Denominamos como “brecha de la complejidad”, a la dificultad que conlleva la asimilación y práctica de dichos requerimientos y que, a su vez, constituye el principal reto al que nos enfrentamos en la actualidad. Por último, desde una perspectiva de desarrollo orgánico (“bottom- up”, y considerando a la persona como “sistema complejo adaptativo”, se propone el perfil “eAprendiz” como estrategia de adecuación y empoderamiento personal, y profesional, en el actual entorno global, “extendido y complejo”.

  14. La subjetividad desde la perspectiva histórico cultural: un tránsito desde el pensamiento dialéctico al pensamiento complejo

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    Oscar G. Hernández

    2008-01-01

    Full Text Available En el presente artículo se expone conceptualmente la categoría de la subjetividad y los elementos que la sustentan desde las propuestas provenientes de la psicología histórico cultural. Para ello se hizo una revisión de las principales ideas de la filosofía del materialismo dialéctico, y de los elementos en la obra de L.Vigotsky que se relacionan con su develamiento. Adicionalmente, se realizó una breve exposición de las contribuciones más destacadas que desde la psicología soviética, facilitaron una concepción particular de la subjetividad humana. Finalmente, se muestran los principios del pensamiento complejo propuesto por E. Morin y su articulación respecto a la propuesta actual de la subjetividad como campo de investigación psicológica.

  15. El tecno-complejo del Embalse de Urrúnaga (Álava. Nuevas aportaciones al conocimiento de las sociedades del Paleolítico inferior en el norte de la Península Ibérica

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    Javier FERNÁNDEZ-ERASO

    2017-01-01

    Full Text Available En este trabajo se presenta una nueva colección lítica adscrita al Paleolítico inferior recuperada en el embalse de Urrúnaga (Álava, País Vasco, España. Consta de 70 artefactos que han sido estudiados conforme a los parámetros de la Tipología Analítica a partir de las estructuras de análisis; Petrológica, Modal-Morfológica, Tecnológica y Tipométrica. A partir de este estudio integral e interdisciplinar se han podido comprender los procesos de captación de materias primas y las diferentes dinámicas de talla a la hora de elaborar los diferentes artefactos. De este modo, el tecno-complejo aquí presentado, supone un nuevo aporte al conocimiento de las comunidades inferopaleoliticas en un territorio donde escasean las evidencias arqueológicas de este periodo cronológico. Además pone de manifiesto la importancia del emplazamiento en el que se sitúa el embalse como un lugar de paso obligado para la comunicación entre el interior meseteño, valle del Ebro y la costa.

  16. Fractura triple del complejo suspensorio del hombro

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    Eraclio Delgado Rifá

    2015-06-01

    Full Text Available El complejo suspensorio del hombro es una estructura sumamente importante, compuesta por un anillo de huesos y tejidos blandos. Las lesiones aisladas de este complejo anatómico son frecuentes y no afectan su estabilidad. La interrupción doble conduce a la inestabilidad de esta estructura y usualmente requiere tratamiento quirúrgico. La triple interrupción, por su parte, es sumamente rara y es encontrada en casos de trauma de alta energía a menudo en asociación con otras lesiones. Se presenta una triple lesión del complejo suspensorio del hombro en un paciente de 46 años de edad, con una fractura de la glenoides, del acromion tipo III de Kuhn y de la coracoides tipo II de Ogawa. En este caso, la fractura del acromion fue tratada con fijación percutánea con alambres de Kirschner, por tener asociado lesiones de partes blandas que contraindicaron la reducción abierta. La fractura de la glenoides y de la apófisis coracoides fueron tratadas conservadoramente. Después de 6 meses de evolución, el paciente tuvo un resultado funcional aceptable, con una abducción de 90 grados, los 30 grados de rotación externa y 70 de rotación interna, además asintomático y consolidación de todas las fracturas. A pesar de ser catalogada de una lesión grave del cinturón escapular y estar asociada a otras lesiones se obtuvo un resultado satisfactorio al final del tratamiento.

  17. Evaluación de la bioseguridad del protocolo de extracción de ADN para especies del complejo Mycobacterium tuberculosis implementado en el Instituto Nacional de Salud

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    Wellman Ribón

    2009-12-01

    Conclusión. Se determinó que el protocolo de extracción de ADN descrito por van Soolingen et al. (2002 e implementado en el Instituto Nacional de Salud de Colombia, es seguro para el personal de laboratorio y el medio ambiente.

  18. Una aproximación al significado biológico del polimorfismo del Complejo Mayor de Histocompatibilidad. El modelo de la asociación HLA y ARJ

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    Gloria Garavito

    2002-01-01

    Full Text Available Objetivos. Llevar a cabo una actualización del estado del arte acerca del significado biológico del polimorfismo del sistema genético Complejo Mayor de Histocompatibilidad (CMH. Revisar la literatura relacionada con la asociación de los antígenos de histocompatibilidad en el humano (HLA y la susceptibilidad o resistencia al desarrollo de la artritis reumatoidea juvenil (ARJ. Presentar un modelo hipotético para la comprensión de la susceptibilidad genética a desarrollar ARJ. Fuente de datos. Las referencia bibliográficas que soportan este artículo se obtuvieron a través de búsquedas en las siguientes bases de datos: Pubmed, Ovid, Ebsco. Inicialmente se encontraron 139 artículos, de los cuales se seleccionaron 75. Se incluyó también información del desarrollo de un proyecto de investigación de nuestro grupo, «Polimorfismo molecular de los antígenos HLA en ARJ» (cod. Colciencias 1215-04-280-96. Resultados. L artritis reumatoidea juvenil (ARJ es la enfermedad más común en la práctica reumatológica pediátrica y la menos estudiada inmunogenéticamente. A diferencia de la artritis reumatoide (AR del adulto, la ARJ tiene ciertas variantes clínicas, lo que la hace más interesante desde el punto de vista genético (fenotipos. En su etiopatogenia se han identificado varios factores que en su conjunto explicarían el inicio y la perpetuación de la respuesta inflamatoria que afecta las articulaciones y tejidos vecinos, y que de no ser controlados llegan a destruirlos, tal como sucede en otras enfermedades autoinmunes. La patogénesis de la enfermedad puede determinarse por alteraciones a nivel de complejo trimolecular, constituido por un antígeno putativo, el receptor de linfocitos T y el Complejo Mayor de Histocompatibilidad (CMH. Nuestro grupo durante los últimos 4 años ha estado estudiando la asociación entre ARJ y el polimorfismo de los alelos HLA DRB1* y DQB1* en niños mestizos de nuestro país. Los resultados son

  19. Efecto de diversos métodos de preprocesamiento matemático al completar datos faltantes en los monitoreos del complejo lagunar Ciénaga Grande de Santa Marta, mediante el enfoque de atípicos aditivos

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    Carvajalino Fernández Marcos A.

    2011-11-01

    Full Text Available Se presentan los resultados de la aplicación de diversas técnicas de preprocesamiento de datos con la finalidad de adecuar las series de tiempo de las variables fisicoquímicas medidas en el complejo lagunar Ciénaga Grande de Santa Marta a los requerimientos mínimos de la metodología de determinación de observaciones faltantes mediante atípicos aditivos a través del software Tramo and Seats for Windows (TSW: TRAMO and SEATS for WINDOWS, Gómez & Maravall, 2009. Las series utilizadas corresponden a los registros históricos de un proyecto de monitoreo para la rehabilitación de la zona entre 1993 y 2008. Se evaluaron cuatro enfoques de preprocesamiento: Interpolación histórica, escalamiento, división y división con interpolación histórica. Los cuatro métodos fueron comparados aplicándolos a 4 variables en 3 estaciones de monitoreo dentro del sistema lagunar. Los métodos de interpolación histórica y escalamiento mostraron resultados favorables al preparar los datos de las series históricas para el uso del método de atípicos aditivos, con valores de cuadrado medio del error (MSE de los residuales entre 0.172 x 102 y 19.28 para la interpolación histórica y entre 0.232 x 102 y 17.818 para el escalamiento. Se recomienda el uso de interpolación histórica en casos de series cortas con vacíos distribuidos aleatoriamente o en estudios donde la frecuencia original del muestreo sea un factor decisivo, mientras que en casos de series con vacíos agrupados y mayor longitud, deben ser tratados mediante escalamiento de la frecuencia de muestreo.

  20. Genética de la resistencia al complejo Phyllachora maydis Maubl., Monographella maydis Müller & Samuels y Coniothyrium phyllachorae Maubl., en diversos genotipos de maíz (Zea mays L.).

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    Hernández Ramos, Lervin

    2014-01-01

    El complejo de la mancha de asfalto del maíz, inducido por los hongos Phyllachora maydis y Monographella maydis, es una enfermedad de importancia económica en México y Centroamérica, debido a que provoca severas pérdidas en el rendimiento y deteriora la calidad del forraje. El mejoramiento genético de la resistencia del hospedante a través de la generación de genotipos resistentes representa la medida de control más eficiente para el control de la enfermedad. Se conoce poco respecto a la base...

  1. Estudio geológico - geotécnico de la inestabilidad del suelo que afecta al Complejo Arqueológico Cojitambo, estabilización y medidas de mitigación

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    Roura Ortega, Martha

    2010-01-01

    Debido a los altos costos de la investigación geotécnica, se realiza esta Tesis utilizando datos de los estudios realizados en el Complejo Arqueológico Cojitambo, a fin de establecer las causas del colapso de la Ruinas y recomendar medidas de estabilización o mitigación. La investigación regional delimita la zona inestable donde se realizan los estudios a detalle. Los factores internos que intervienen son: las propiedades geotécnicas del material que aflora y el diaclasamiento de la roca suby...

  2. Síndrome doloroso regional complejo: Conceptos actuales

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    Ferrer Lozano, Yovanny; Oquendo Vázquez, Pablo; Ferrer Lozano, Dunia

    2006-01-01

    Se realizó una revisión bibliográfica de los conceptos actuales que se les atribuyen al síndrome doloroso regional complejo, término propuesto en 1994 por la International Association for Study of Pain. En la aparición del síndrome intervienen varios factores como son los sistemas nerviosos periférico, central y vegetativo; el sistema endocrino, los factores psicológicos, históricos, ambientales y circunstanciales; que permiten en conjunto personalizar el dolor en cada paciente.El tratamiento...

  3. Complejo industrial, en Sparanise, Italia

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    Figini, Luigi

    1966-02-01

    Full Text Available In the neighbourhood of Saranise an industrial group of buildings has been built, which affects four distinct types of industries, and occupies a ground area of 850.000 m2 close to the national Appia roadway. This industrial complex is served by roads, water, light and fuel supplies, garages, workshops, offices, etc. The project was initiated by Pozi Ceramic Manufacturers. Care has been taken that the general architectural style for all the buildings shall be the same, and that the project as a whole shall exhibit a unity of pattern. Although the structure of the buildings is very similar, since all involve prestressed beams for the roofs, details vary in each particular case, depending on the spans to be covered. A special feature is the roofing of the varnishes factory, which is covered with precast, V shaped units, made in a nearby workshop. These large units have been handled by a travelling crane, which has lifted them to their final emplacement. The units weigh between 13 and 14 tons each. A specialised firm made a study of the ground, and calculated the maximum permissible loadings. They then decided on the most suitable type of foundations in each case.En las inmediaciones de Sparanise se ha construido un complejo fabril, constituido por cuatro grupos de industrias diferentes, cuyos edificios ocupan un solar de 850.000 m2, inmediato a la carretera nacional Appia, dotado de todos los servicios, redes de viales, de agua, luz y combustibles, garaje, talleres, oficinas, etc. La empresa iniciadora es la Manufactura Cerámica Pozzi. Se ha cuidado de que la línea general arquitectónica para los distintos edificios que componen cada grupo sea homogénea, y de que el conjunto tenga un tratamiento semejante que le dé la debida unidad. Aunque las estructuras son muy similares, pues todas ellas se componen de vigas pretensadas para los forjados de cubierta, aquéllas varían de una a otra nave, en cada grupo, de acuerdo con las luces previstas

  4. Salud: un sistema complejo adaptativo Health: an adaptive complex system

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    Luis Fernando Toro-Palacio

    2012-02-01

    Full Text Available Este artículo destaca la enorme distancia existente entre el pensamiento complejo de índole intelectual, difundido en nuestro medio, y el pensamiento complejo de índole experimental, que ha permitido lograr los desarrollos científico-tecnológicos que han cambiado radicalmente el mundo. Invita a considerar como sistemas complejos adaptativos entidades tales como la vida, el ser humano, la sociedad global y todo aquello que se llama salud, para lo cual resulta prioritario adoptar un enfoque diferente que amplíe su conocimiento. Al reconocer esta racionalidad, se sustentan las principales características y propiedades emergentes de la salud como sistema complejo adaptativo, siguiendo un modelo de prestación de cuidados y servicios. Finalmente, se plantean algunas preguntas de investigación pertinentes desde esta perspectiva, y se expresan una serie de apreciaciones que se espera sirvan para comprender todo lo que como individuos y como especie hemos llegado a ser. Se propone en este trabajo que la salud y la prestación de servicios de asistencia sanitaria se consideren como sistemas complejos adaptativos.This article points out the enormous gap that exists between complex thinking of an intellectual nature currently present in our environment, and complex experimental thinking that has facilitated the scientific and technological advances that have radically changed the world. The article suggests that life, human beings, global society, and all that constitutes health be considered as adaptive complex systems. This idea, in turn, prioritizes the adoption of a different approach that seeks to expand understanding. When this rationale is recognized, the principal characteristics and emerging properties of health as an adaptive complex system are sustained, following a care and services delivery model. Finally, some pertinent questions from this perspective are put forward in terms of research, and a series of appraisals are expressed that

  5. ¿Inecuaciones en los complejos?

    OpenAIRE

    Guacaneme, Edgar Alberto

    2000-01-01

    En tanto profesores de matemáticas, presentamos aquí una reflexión que intenta cuestionar la imposibilidad de definir y solucionar inecuaciones en los números complejos. Así, en primer lugar, asumimos como objeto de provocación la existencia de un conjunto-solución para una determinada "inecuación" de variable compleja. En segundo lugar, como consecuencia de haber cuestionado el uso de la relación de orden usual de los números reales en el campo de los números complejos, recapitulamos un crit...

  6. La organización empresarial como sistema adaptativo complejo

    Directory of Open Access Journals (Sweden)

    Luz Esperanza Bohórquez Arévalo

    2013-01-01

    Full Text Available El presente documento busca identificar las características de los sistemas adaptativos complejos (CAS, por su sigla en inglés Complexity Adaptative Systems , así como capturar las implicaciones de su aplica- ción en el estudio de las organizaciones. Para tal fin, se revisan resultados de investigación publicados en revistas académicas que han facilitado la introducción de las ciencias de la complejidad en la adminis- tración, al igual que se exploran los resultados de investigación de grupos e institutos que trabajan en el tema. A partir de los estudios comparativos de las propuestas observadas se plantea que los CAS pueden comprenderse desde la emergencia, la auto-organización y la evolución. La comprensión de la organiza- ción desde esta perspectiva genera líneas de investigación en liderazgo, estrategia, toma de decisiones y construcción de actuación conjunta; todo lo anterior en sistemas complejos.

  7. La organización empresarial como sistema adaptativo complejo

    Directory of Open Access Journals (Sweden)

    Luz Esperanza Bohórquez Arévalo

    2013-04-01

    Full Text Available El presente documento busca identificar las características de los sistemas adaptativos complejos (CAS, por su sigla en inglés Complexity Adaptative Systems, así como capturar las implicaciones de su aplicación en el estudio de las organizaciones. Para tal fin, se revisan resultados de investigación publicados en revistas académicas que han facilitado la introducción de las ciencias de la complejidad en la administración, al igual que se exploran los resultados de investigación de grupos e institutos que trabajan en el tema. A partir de los estudios comparativos de las propuestas observadas se plantea que los CAS pueden comprenderse desde la emergencia, la auto-organización y la evolución. La comprensión de la organización desde esta perspectiva genera líneas de investigación en liderazgo, estrategia, toma de decisiones y construcción de actuación conjunta; todo lo anterior en sistemas complejos.

  8. Evaluando y fomentando el desarrollo cognitivo y el aprendizaje complejo

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    Sandra Castañeda Figueiras

    2004-01-01

    Full Text Available Se abordan los desarrollos investigativos originales que han traducido premisas teóricas acerca de la cognición humana a marcos de trabajo y herramientas tecnológicas aplicables al campo educativo. Los artefactos han sido diseñados para abrir la caja negra del qué, del cómo y del hasta dónde de la oferta generada en los niveles educativos medio superior y superior en México. Marcos y herramientas se fundan en la actividad cognitiva constructiva autorregulada, apoyan la medición y el fomento de resultados del aprendizaje complejo y de sus habilidades asociadas. La asunción subyacente considera que la aplicación de premisas cognitivas al diseño de herramientas educativas es central y que, en este contexto, es fundamental asegurar la estrecha relación entre la investigación y la práctica educativa ordinaria, a fin de apoyar los cambios deseados. En este trabajo se argumentan fundamentos teóricos, se aportan evidencias en apoyo a los desarrollos generados y se enfatiza la necesidad de extender investigación que identifique mecanismos responsables del aprendizaje efectivo en escenarios reales, con el fin de lograr su comprensión cabal y generar alternativas ecológicamente válidas y útiles.

  9. Complejo Omni - Atlanta – (EE. UU.

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    Housworth, Marvin

    1976-12-01

    Full Text Available This complex of buildings, situated in the centre of Atlanta City, forms a dynamic nucleus for various social activities, such as recreational, commercial and business activities. These buildings are constructed above railway nets, due to special property rights for this lot, which constituted one of the main determinants for the characteristics of the property. The unit is made up by a luxurious hotel, two restaurants, office buildings and shopping arcades, arranged around a spacious inner yard. This patio is covered by means of an exceedingly big glass roof, supported by beams and steel framework and is provided with walls of big glazed surfaces. Thus, an intimate and friendly atmosphere is created, free from the contamination and noise of the big city whereby the square displays the typical characteristics of open squares in smaller towns.Este conjunto de edificios, emplazado en el centro de la ciudad de Atlanta, conforma un núcleo dinámico en donde se encuentran diversas actividades de tipo social: recreativas, comerciales y empresariales. Se ha construido sobre ruedas ferroviarias, en virtud de derechos especiales de propiedad que conservaba la parcela, lo que constituyó uno de los principales condicionamientos de fas características del proyecto. El complejo dispone de un hotel de lujo, dos restaurantes, edificios de oficinas y galerías comerciales, dispuestos en torno a un amplio espacio interior, cerrado por una enorme cubierta acristalada, apoyada en vigas y entramados metálicos, y por grandes ventanales corridos entre bloques. Conforma así un ambiente íntimo y acogedor, liberado del ruido y de la atmósfera turbulenta de la gran ciudad, con características propias de las pequeñas plazas populares.

  10. Complejo administrativo comercial – Lausana, Suiza

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    Willomet, R.

    1976-07-01

    Full Text Available Administrative and commercial complex - Lausanne - Switzerland In an area which is being rebuilt in the centre of Lausanne, a 145,000 m2 administrative and commercial complex has been built. It consists of a low body with 3 levels, arranged as platforms above the street and in the shape of terraces with gardens whereby a 4,000 m2 area reserved for pedestrians has been created. On this lower part, high buildings have been constructed in harmony with each other. The building system consists of an upper structure with big beams and ribs which support the façade panels. The structure rests upon 4 big central columns and two on the side, whereby a diaphanous look has been achieved, further increased by mobile partition walls by which future needs also can be attended to. The light exterior enclosures were thoroughly studied and tested so as to provide total protection against dust and outer noise. Further the construction is equipped with interior heating and cooling installations and insulation from sound. This new urban complex does not attempt to adapt itself to the surrounding environment, but its lines are rather an expression of the architecture of tomorrow.En la ciudad de Lausana se ha construido un complejo administrativo y comercial de 145.000 m2, en una zona urbana del centro sujeta a remodelación. Consta de un cuerpo bajo, con tres niveles, a modo de plataformas sobre la calle, creándose así una zona de 4.000 m2 reservada a los peatones, con lugares para pasear, hacer compras o descansar entre terrazas ajardinadas. Sobre este volumen bajo se elevaron cuerpos en altura, combinados adecuadamente para constituir un conjunto estético y armonioso.

  11. La producción familiar en el complejo avícola entrerriano: trayectoria, estrategias y transformaciones

    OpenAIRE

    García , Ana Laura

    2012-01-01

    El presente trabajo aborda el análisis de las estrategias desplegadas por los productores familiares integrados contractualmente al complejo avícola entrerriano a lo largo de sus cursos de vida. En particular, interesa profundizar tres ejes: los cambios y continuidades en sus estrategias, sus implicancias en términos de la permanencia de la agricultura familiar y su reconfiguración interna.

  12. Biodiversidad del complejo de artrópodos asociados al follaje de la vegetación del norte de Chile, II región Biodiversity of the canopy arthropods associated to vegetation of the north of Chile, II region

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    FRANCISCO SAIZ

    2000-12-01

    Full Text Available Uno de los componentes principales de la biodiversidad de los ecosistemas corresponde al complejo plantas hospederas, fitófagos y organismos que regulan a las poblaciones de fitófagos. El conocimiento de este complejo en la región árida de Chile es escaso, situación que motiva la presente investigación. Como hipótesis se plantea que a más favorables condiciones ambientales, a mayor diversidad de plantas hospederas y a mayor contenido de nitrógeno y de agua en los vegetales se sustentará una mayor biodiversidad de fitófagos, los que, a su vez, sustentarán mayor diversidad de depredadores y de parasitoides. El estudio se realizó en la II Región del país, mediante un transecto longitudinal, desde la Costa a la Cordillera de los Andes, el que cruza las siguientes zonas bioclimáticas: Desierto Litoral (DL, Desierto Interior (DI, Tropical Marginal (TM Tropical de Altura (TA, con un gradiente altitudinal de 0 a 4.000 msnm. La colecta de los artrópodos del follaje se hizo mediante la técnica del paraguas entomológico sobre las plantas vegetativamente activas y los formadores de cecidias, minadores y defoliadores por colecta directa de los órganos afectados. En total se colectó e identificó un total de 97 especies vegetales distribuidas en 28 familias. Desde este punto de vista, la zona ecológica más pobre es el DI, destacando DL y TM como las zonas más ricas en vegetación. Sin embargo, existe fuerte aislamiento de la vegetación del DL y una casi total sobreposición de la vegetación del DI con la de la zona TM. En cuanto a familias, la mayor riqueza específica la presenta Asteraceae, seguida por Chenopodiaceae, Solanaceae, Nolanaceae, Fabaceae y Portulacaceae. Asteraceae y Chenopodiaceae destacan, además, por estar presentes en las cuatro zonas ecológicas. Desde el punto de vista nutricional, el contenido foliar de agua y de cenizas de la vegetación varía considerablemente, con valores promedio extremos en el DL y en TA

  13. Algunos complejos de carboxilatos de uranio (IV y 1-pirazolilborato

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    Omar Velasquez

    2009-07-01

    Full Text Available En este trabajo se presenta la síntesis y caracterización, mediante espectroscopia de infrarojo y H'—NMR de complejos de acetato de uranio, dietil carbamatos y dietilditiocarbamatos y 1—pirazolil—borato.

  14. Análisis comparativo de seis genomas del complejo Mycobacterium tuberculosis

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    Diego Chaves

    2010-03-01

    Conclusiones. A pesar de la gran similitud entre las cepas analizadas, existen variaciones entre ellas que pueden ser importantes para entender diferencias en comportamiento y virulencia, así como para mejorar los diagnósticos de cepas específicas. Regiones como aquéllas con genes para proteínas de membrana, posiblemente, relacionadas con la variación y la respuesta antigénica, son de particular interés para estudios futuros orientados a buscar tratamientos nuevos para el control de esta enfermedad.

  15. Congruencia del pensamiento complejo de Edgar Morín en la metodología de la educación a distancia

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    Monica de Jesus Chacón-Prado

    2015-09-01

    Full Text Available El filósofo francés Edgar Morín propone estudiar la verdad a través del pensamiento complejo. Este es un abordaje al conocimiento que apoya el uso de múltiples perspectivas o enfoques y niega la fragmentación del objeto durante su estudio. Por ello, este documento escribe desde la visión filosófica como el pensamiento complejo es especialmente útil en la educación a distancia porque fomenta convivir con la incertidumbre y la inestabilidad del mundo de hoy.

  16. “Tipificación molecular y diversidad genética de Mycobacterium spp”

    OpenAIRE

    Marín Hernández, David

    2012-01-01

    La Tuberculosis sigue siendo la enfermedad infecciosa con mayor prevalencia en el mundo con un tercio de la población infectada por algún miembro del complejo Mycobacterium tuberculosis. La enfermedad se ha expandido en los países en desarrollo y para el 2005 en México la incidencia fue de 15,249 nuevos casos pulmonares confirmados en el laboratorio. Aunque Mycobacterium tuberculosis es el agente causal principal de la tuberculosis humana, se han reportado continuamente caso...

  17. Caracterización tecnomorfológica del complejo inferior de Ambrona

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    Joaquín Panera Gallego

    1996-01-01

    Full Text Available La industria achelense correspondiente al Complejo Inferior de Ambrona, atribuida al Pleistoceno medio, se sitúa en la órbita de la de Áridos, La Maya II, El Sartalejo y Torralba. Mediante su caracterización tecnomorfológica y su contextualización en el Paleolítico inferior de la Península Ibérica, pretendemos paliar la carencia originada por la falta de trabajos en profundidad sobre su estudio.The Acheulian industry of «The Lower Compiex» in Ambrona, appointed to the Middle Pleistocene, is situated in the orbit of Áridos, La Maya II, El Sartalejo and Torralba. We propose palliate the lacks, originated for the non-existence ofjobs about his study, by his technomorphological analysis in the Lower Paleolithic of the Iberian Península.

  18. In vitro susceptibility of Mycobacterium tuberculosis, Mycobacterium africanum, Mycobacterium bovis, Mycobacterium avium, Mycobacterium fortuitum, and Mycobacterium chelonae to ticarcillin in combination with clavulanic acid.

    OpenAIRE

    Casal, M J; Rodriguez, F C; Luna, M D; Benavente, M C

    1987-01-01

    The in vitro susceptibility of Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium avium, Mycobacterium fortuitum, and Mycobacterium chelonae (M. chelonei) to ticarcillin in combination with calvulanic acid (CA) was studied by the agar dilution method. All the M. tuberculosis, M. bovis, and M. africanum strains were inhibited at a ticarcillin concentration of 32 micrograms/ml or lower in combination with 5 micrograms of CA. M. chelonae and M. avium strains ...

  19. Una introducción a los números complejos

    OpenAIRE

    Rivero, Francisco

    2004-01-01

    Una introducción a los números complejos (Rivero, Francisco) Historia de los números complejos 1.1 Los inicios del álgebra 1.2 Cardano 1.3 Bombelli 1.4 Números imaginarios Álgebra de los números complejos 2.1 Definición de número complejo 2.2 Suma de números complejos 2.3 Producto de números complejos 2.4 Representación geométrica 2.5 La forma polar 2.6 Potencias y raíces de números complejos 44 ciens-004 Primera Nivel monográ...

  20. The Mycobacterium tuberculosis homologue of the Mycobacterium ...

    African Journals Online (AJOL)

    With the completion of genome sequencing of Mycobacterium tuberculosis and upsurge in the incidence of M. tuberculosis infection worldwide partly as a result of HIV pandemic, there is need for rationale approach to vaccine and chemotherapy discoveries for M. tuberculosis. The homologue of mig gene of. Mycobacterium ...

  1. Evaluación in vitro de tabletas orales de complejos de cobre con antiinflamatorios no esteroideos

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    Julie Fernanda Benavides Arévalo

    Full Text Available Introducción: los antiinflamatorios no esteroideos (AINES, son un grupo diverso y químicamente heterogéneo de fármacos analgésicos, antipiréticos y antiinflamatorios; sin embargo, su funcionamiento se ve afectado por su baja solubilidad acuosa y por la incidencia de efectos secundarios gastrointestinales. Como una alternativa a este problema, la formulación de tabletas orales con complejos de cobre con AINES muestra un adecuado comportamiento in vitro y podría tener efectos secundarios reducidos comparados con el fármaco antiinflamatorio no complejado. Objetivo: evaluar los procesos de desintegración y de disolución de tabletas orales de complejos de cobre con AINES. Métodos: luego de la síntesis y la caracterización instrumental de los complejos, se evaluó por microscopia óptica el efecto de la morfología, el tamaño de partícula y las características superficiales en el proceso de disolución; estos resultados fueron considerados para la formulación de los complejos y permitieron junto con la adecuada elección de un desintegrarte, un modificador de flujo, un lubricante y un aglutinante, la obtención de tabletas por compresión directa. Finalmente, en los comprimidos se evaluaron propiedades fisicomecánicas y los procesos de desintegración y disolución. Resultados: las tabletas de los complejos mostraron tiempos de desintegración entre 5 y 15 min y una liberación in vitro del 75 al 93 %. Conclusiones: la disolución de los AINES se vio favorecida por la formación de complejos, el menor tamaño de partícula, la presencia de poros y grietas en la superficie de las partículas y la inclusión de excipientes para la formulación de tabletas orales.

  2. Consideraciones básicas del pensamiento complejo de Edgar Morin, en la educación

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    José Miguel Pereira-Chaves

    2010-06-01

    Full Text Available Recibido 18 de noviembre de 2009 • Aceptado 02 de diciembre de 2009 • Corregido 09 de mayo de 2010     Este ensayo consiste en una breve descripción y un análisis del pensamiento complejo de Edgar Morin; para ello se hace mención al enfoque sistémico el cual se refiere a la interconexión y a las interacciones entre los objetos, las personas y el ambiente como un todo, lo cual sirve de fundamento para la propuesta educativa, en la que el conocimiento se debe abordar de manera integral y no fragmentado. Por lo anterior, el conocimiento humano debe fomentar la toma de conciencia de que somos tan sólo una parte componente de un sistema más general (complejo, y en constante interacción, en el que la educación puede ser el medio efectivo para que los alumnos desarrollen la capacidad de comprender otros sistemas complejos, además de los seres vivos. Aquí cobran importante interés las “nuevas ciencias” y las humanidades.

  3. ¿Tecnofilia, alternidad o pensamiento ácrata? Metamediación y periodismo complejo

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    Yamile Haber Guerra

    2014-09-01

    Full Text Available Este documento contiene una discusión sobre el periodismo y la tecnología en la sociedad contemporánea. Trae a debate la relación entre el periodismo,  la internet y los nuevos lenguajes. En este contexto, la información asume nuevas funciones y fines informativos que el canon tradicional no había previsto. Las formas clásicas de construcción de la noticia se erigen sobre los nuevos paradigmas con efectividad y pertinencia aún no documentados del todo. El texto invita al reconocimiento de la complejidad como principio estructurante de todo saber comunicacional, y del lenguaje periodístico como objeto de estudio amplio, complejo y abierto a nuevos métodos no excluyentes, concurrentes e integrados.

  4. Abordar lo complejo desde el diseño: una mirada hacia la transdisciplinariedad

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    Leonardo Andrés Moreno Toledano

    2017-07-01

    Full Text Available El presente ensayo plantea una reflexión sobre la dificultad que las disciplinas actuales presentan al enfrentarse a los problemas complejos de nuestra era. Para ello, se plantea la necesidad de abordar los fenómenos actuales desde enfoques no tradicionales y desarrollados a raíz de algunas teorías que han sido creadas con el fin de explicar este tipo de problemas. Asimismo, en el artículo se resalta el potencial del diseño, como disciplina orientada a la práctica, para encontrar soluciones a diversos fenómenos que se suceden en nuestras sociedades a partir de visiones no unidisciplinares.

  5. Diversidad alfa y beta de la comunidad de reptiles en el complejo cenagoso de Zapatosa, Colombia

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    Guido Fabián Medina-Rangel

    2011-06-01

    Full Text Available La diversidad es una propiedad de las comunidades, permite describir, caracterizar y entender el funcionamiento de los ecosistemas. Para estudiar la riqueza, abundancia y el recambio de especies de reptiles entre hábitats circundantes al complejo cenagoso de Zapatosa, se realizaron cuatro salidas de campo (noviembre de 2006 y octubre de 2007. Se invirtieron 640h/hombre de muestreo repartido en cinco tipos de hábitat: bosque casmófito, bosque seco, bosque de ribera, palmar y sabana arbolada. Se encontraron 847 individuos de 48 especies, la familia más rica fue Colubridae, se registraron cinco especies endémicas y ocho con algún grado de amenaza en conservación a nivel nacional. El hábitat más diverso fue el bosque de ribera. La representatividad del muestreo en total y para cada hábitat fue superior al 80%, con excepción del palmar (<75%. La composición de especies fue diferente entre la sabana arbolada y los hábitats bosque de ribera, palmar y bosque seco. El recambio promedio de especies fue del 50%. El bosque casmófito mostró la mayor complementariedad y número de especies únicas. El complejo cenagoso presentó 2/3 de los reptiles registrados hasta hoy para la región Caribe y más del 80% de los encontrados para el departamento del Cesar.

  6. El estudio de sistemas económicos complejos

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    Guillermo De León Lázaro

    2012-01-01

    Full Text Available Una y otra vez, un año tras otro, una generación después de otra, los investigadores que trabajan en el ámbito de la economía y gestión de empresas, han intentado encauzar sus esfuerzos hacía la búsqueda de un cuerpo científico capaz de comprender mejor, explicar más adecuadamente y tratar con rigor los fenómenos, cada vez más complejos, que mueven la vida de los estados, de las instituciones y de las empresas.

  7. SISTEMAS COMPLEJOS, COMPUTACION EVOLUTIVA Y APLICACIONES A LA PLANIFICACION MINERA

    OpenAIRE

    Goles Chacc, Eric Antonio

    2013-01-01

    El secuenciamiento óptimo (extracción sucesiva en el tiempo de los bloques de la mina, en términos económicamente más conveniente) está directamente asociado a la parte más relevante del negocio minero. Del punto de vista del Modelamiento y su resolución por medio de algoritmos complejos, este problema no está bien resuelto en la industria actual, constituyéndose así en un dominio de investigación aplicada muy relevante. El objetivo del proyecto es generar una plataforma de servicios de...

  8. SIMULACIÓN DE MECANISMOS COMPLEJOS DE REACCIÓN.

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    Daniel A Barragán R.

    2010-10-01

    Full Text Available Se propone un método para la interpretación de mecanismos complejos de reacción, el cual es aplicado a la simulación del mecanismo de Gyórgyi-Field, (GF desarrollado para la reacción química oscilante de Belousov-Zhabotinsky (BZ. El método basado en la ley de acción de masas y en el principio de independencia y coexistencia de los pasos elementales, reproduce el comportamiento oscilatorio en la concentración de los intermediarios de reacción.

  9. Molecular Epidemiology of Mycobacterium Tuberculosis Strains in ...

    African Journals Online (AJOL)

    Doroudchi M, Kremer K, Basiri EA, Kadivar MR,. Van Soolingen D, Ghaderi AA. IS6110‑RFLP and spoligotyping of Mycobacterium tuberculosis isolates in Iran. Scand J Infect. Dis 2000;32:663‑8. 13. Farnia P, Masjedi MR, Mirsaeidi M, Mohammadi F,. Jallaledin‑Ghanavi, Vincent V, et al. Prevalence of Haarlem I and Beijing ...

  10. Caracterización del complejo mayor de histocompatibilidad clase II en primates del género Aotus

    OpenAIRE

    Suárez Martínez, Carlos Fernando

    2017-01-01

    El presente trabajo tiene como propósito contribuir al conocimiento del complejo mayor de histocompatibilidad clase II (CMH-II) de los monos Aotus, contribuyendo a la validación de este primate como modelo experimental, y aumentando el conocimiento en la evolución de los genes del CMH en primates. Además, se profundizó en el análisis de convergencia y polimorfismo de los genes del CMH-DR en primates. Se implementaron metodologías de modelación computacional de la unión CMH-péptido, como ...

  11. Evaluación de complejos enzimáticos en alimentación de pollos de engorde

    OpenAIRE

    García Jiménez, Mario

    2011-01-01

    El objetivo de esta tesis doctoral ha sido evaluar la adición de diversos complejos enzimáticos a dietas basadas en cereales que contenían diferentes cantidades de polisacáridos no amiláceos (PNA), sobre la productividad y parametros digestivos de pollos de engorde. Asimismo se ha estudiado la variación de la respuesta a la suplementación enzimática(SE) en función de otros factores tales como el periodo de ayuno posterior al nacimiento, el procesamiento termico del cereal, el tipo de cereal u...

  12. Las organizaciones de enfermería como sistemas complejos

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    Caro Castillo Clara Virginia

    2002-10-01

    Full Text Available

    This study takes into account some considerations about the classical logic when building knowledge. It states the logic of complexity, the complex thought and the rational, open and complex systems, as a new paradigm. In the light of the organization concept proposed by Morin, complex adaptative systems are emphasized, as well as other terms such as order, disorder, incidental events and noises. The self-eco-organizer process, takes the human being thinking as starting point and as dynamizer of simple and complex processes in the construction of knowledge about reality. Some research studies such as those of Erdmann, Meirelles and Pradebon show some ways in which one can learn from the organizational reality of nursing, the exercise of complex thinking in uncertainty, in tenous limits, in heterogeneity, diversity and pluralism of phenomena in its aspect, interactions and associations. The inter subjectivity states a new concept of space and of temporality. The care system organization in nursing visualizes several dimensions of "caríng": to care for himself, to care for himself together with the other, to be looked after by the other, to feel and the process of personal care system, the body itself as the nucleus of care, the fact of belonging to the system of multiple caring relations and the care for nature getting integrated to other beings and other social, natural systems, and in thisway strengthen the feeling of ownership, in search of a better survival, vitality, life human quality.

    El presente estudio hace algunas consideraciones sobre la lógica clásica en la construcción del conocimiento. Señala la lógica de la complejidad, el pensamiento complejo y los sistemas racionales, abiertos y complejos como un nuevo paradigma. A la luz de la concepción de organización propuesta por Morin, enfatiza los sistemas adaptativos complejos, el orden, desorden, eventos, incidentes y ruidos. El proceso auto-eco-organizador toma como

  13. VARIABILIDAD MORFOLÓGICA DE LAS ESPECIES DEL COMPLEJO POA RESINULOSA (POACEAE Y SU RELACIÓN CON LAS ESPECIES DE LA SECCIÓN DIOICOPOA

    Directory of Open Access Journals (Sweden)

    M. Gabriela Fernández Pepi

    2008-01-01

    Full Text Available El complejo Poa resinulosa incluye un grupo de especies conformado por P. calchaquiensis, P. pedersenii y P. resinulosa, de difícil delimitación debido a que las entidades son afines en cuanto a su morfología, aunque presentan distribución disyunta. Para evaluar y analizar a las especies del complejo Poa resinulosa y especies afines al mismo complejo, se midieron 40 caracteres morfológicos y anatómicos en ejemplares de Poa calchaquiensis, P. lanigera, P. pilcomayensis y P. resinulosa. Esos datos se incorporaron a la matriz del conjunto de especies de Poa sección Dioicopoa confeccionada por Giussani (2000, incluyendo también a P. nubensis una nueva especie afín a P. calchaquiensis. Mediante el análisis de componentes principales y pruebas estadísticas, se circunscribió a las especies del complejo Poa resinulosa. Se describió a P. resinulosa como una variedad de P. ligularis, de la que se diferencia por poseer lígula corta, hojas y cañas de menor longitud, menor ancho de la lámina y estomas de menor tamaño. Se presenta una clave para la identificación de Poa ligularis var. resinulosa y taxones afines, y un mapa con la distribución geográfica de ambas variedades de P. ligularis.

  14. Microaerobic growth and anaerobic survival of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum

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    Amy Herndon Lewis

    2015-01-01

    Full Text Available Representative strains of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum (MAIS grew at equal rates in laboratory medium at 21% (air and 12% oxygen. Growth in 6% oxygen proceeded at a 1.4–1.8-fold lower rate. Colony formation was the same at 21% (air and 6% oxygen. The MAIS strains survived rapid shifts from aerobic to anaerobic conditions as measured by two experimental approaches (Falkinham (1996 [1]. MAIS cells grown aerobically to log phase in broth were diluted, spread on agar medium, and incubated anaerobically for up to 20 days at 37 °C. Although no colonies formed anaerobically, upon transfer to aerobic conditions, greater than 25% of the colony forming units (CFU survived after 20 days of anaerobic incubation (Prince et al. (1989 [2]. MAIS cells grown in broth aerobically to log phase were sealed and vigorous agitation led to oxygen depletion (Wayne model. After 12 days anaerobic incubation, M. avium and M. scrofulaceum survival were high (>50%, while M. intracellulare survival was lower (22%. M. avium cells shifted to anaerobiosis in broth had increased levels of glycine dehydrogenase and isocitrate lyase. Growth of MAIS strains at low oxygen levels and their survival following a rapid shift to anaerobiosis is consistent with their presence in environments with fluctuating oxygen levels.

  15. Microaerobic growth and anaerobic survival of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum.

    Science.gov (United States)

    Lewis, Amy Herndon; Falkinham, Joseph O

    2015-03-01

    Representative strains of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum (MAIS) grew at equal rates in laboratory medium at 21% (air) and 12% oxygen. Growth in 6% oxygen proceeded at a 1.4-1.8-fold lower rate. Colony formation was the same at 21% (air) and 6% oxygen. The MAIS strains survived rapid shifts from aerobic to anaerobic conditions as measured by two experimental approaches (Falkinham (1996) [1]). MAIS cells grown aerobically to log phase in broth were diluted, spread on agar medium, and incubated anaerobically for up to 20 days at 37°C. Although no colonies formed anaerobically, upon transfer to aerobic conditions, greater than 25% of the colony forming units (CFU) survived after 20 days of anaerobic incubation (Prince et al. (1989) [2]). MAIS cells grown in broth aerobically to log phase were sealed and vigorous agitation led to oxygen depletion (Wayne model). After 12 days anaerobic incubation, M. avium and M. scrofulaceum survival were high (>50%), while M. intracellulare survival was lower (22%). M. avium cells shifted to anaerobiosis in broth had increased levels of glycine dehydrogenase and isocitrate lyase. Growth of MAIS strains at low oxygen levels and their survival following a rapid shift to anaerobiosis is consistent with their presence in environments with fluctuating oxygen levels. Copyright © 2015 Asian African Society for Mycobacteriology. Published by Elsevier Ltd. All rights reserved.

  16. Crítica desde el pensamiento complejo a los métodos cuantitativos para el análisis educativo. El caso de los rendimientos educativos

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    Carlos Ricardo Aguilar Astorga

    2017-07-01

    Full Text Available En materia de política educativa es común asociar el nivel salarial de un sujeto respecto de su nivel educativo: sin embargo, dicha relación no es tan lineal como parece, sino que, visto desde el pensamiento complejo, intervienen factores como los costos marginales -a los que cada individuo se enfrenta al asistir a su centro educativo-, los cuales comprueban otra reciprocidad de variables. La forma como se construyen las asignaciones presupuestales en materia educativa en México no se deriva de este hecho, sino que hallan una correlación entre rendimiento educativo y asignación presupuestal por nivel educativo, excluyendo factores explicativos que el pensamiento complejo permite destacar. Tomar esto en consideración, mejoraría la política pública educativa.

  17. De sistema mecánico a sistema tecnológico complejo. El caso de los automóviles

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    Arturo Ángel Lara Rivero

    2014-01-01

    Full Text Available La mayoría de los trabajos de investigación relacionados con el sector automotriz se ha concentrado en el análisis de la naturaleza mecánica de los vehículos y, de una manera muy escasa, al estudio de su naturaleza electrónica. Para dar cuenta de las transformaciones profundas ocurridas en las últimas cuatro décadas es necesario integrar en la explicación la manera en que los componentes y los sistemas electrónicos han transformado la naturaleza de los vehículos automotores. El objetivo de este trabajo es analizar la naturaleza de los procesos evolutivos implicados en la transición del vehículo automotor: de un sistema complejo a un sistema complejo adaptable.

  18. Presentación de los números complejos : enfoques e interpretaciones

    OpenAIRE

    Bustamante Donayre, Luis Alberto

    2000-01-01

    Desde que los números complejos aparecieron en el siglo XVI, tardaron dos siglos en ser, en cierta manera, aceptados. Aunque las aportaciones formales de Bombelli, Wessel, Argand, Gauss, Euler y Hamilton contribuyeron a una aceptación parcial de los matemáticos, los incomprendidos números complejos todavía eran considerados por los matemáticos del siglo XIX como entes espurios1 El presente trabajo tiene por objeto encontrar un enfoque en donde los números complejos aparez...

  19. Application of radioisotopes to the investigation of the kinetics and mechanism of reactions of some inorganic complex compounds; Application des radioisotopes a l'etude de la cinetique et du mecanisme des reactions de certains complexes inorganiques; Primenenie radioizotopov k issledovaniyam kinetiki i mekhanizma reaktsij nekotorykh neorganicheskikh slozhnykh soedinenij; Aplicacion de los radioisotopos al estudio de la cinetica y del mecanismo de las reacciones de algunos complejos inorganicos

    Energy Technology Data Exchange (ETDEWEB)

    Harris, Gordon M [Department of Chemistry, University of Buffalo, Buffalo 14, NY (United States)

    1962-03-15

    certains atomes ou radicaux, dans des reactions inorganiques pour lesquelles il faut choisir entre differentes explications, est souvent facilitee par l'emploi d'indicateurs radioactifs. C'est ainsi qu'on a pu prouver que le cyanure n'est pas un produit intermediaire qui subit un echange rapide dans l'oxydation de l'ion thiocyanate par le peroxyde d'hydrogene, quoique le cyanure soit un produit final important de la reaction. (author) [Spanish] Los radioisotopos constituyen medios auxiliares de incalculable valor para el estudio de muchos tipos de reacciones de la quimica inorganica, especialmente de aquellas en que intervienen iones complejos en solucion acuosa. Uno de sus aspectos mas importantes es la determinacion de las velocidades de reaccion y del mecanismo de los intercambios isotopicos. Por ejemplo, el autor ha realizado una serie de estudios sobre el intercambio entre carbonato ''libre'' marcado con carbono-14 y carbonato ligante en iones complejos octaedricos del tipo CoA{sub 2}CO{sub 3}{sup +}, donde A = 2NH{sub 3}, etilendiamina (en), propilendiamina (pn) o trimetilendiamina (tn). Estos estudios han permitido comprender a fondo la naturaleza de las reacciones de sustitucion de ligantes de este tipo y del papel que desempenan en el proceso los ligantes amino que no experimentan intercambio. Igualmente fructiferos han sido unos estudios analogos del intercambio de restos oxalato en complejos trioxalicos de formula M (C{sub 2}O{sub 4}){sub 3}{sup 3-}, en la que M = Co (III), Cr (III) o Rh (III). Otra tecnica, que puede aplicarse solamente cuando se emplean radioisotopos de bajo peso atomico, tales como el carbono-14, consiste en observar los efectos de equilibrio o los efectos cineticos que los isotopos ejercen sobre la reaccion. Como ejemplo, el autor ha logrado interpretar diferencias entre los mecanismos de intercambio de los restos carbonato ligantes entre el Coen{sub 2}CO{sub 3}{sup +} y el Cotn{sub 2}CO{sub 3}{sup +} por medicion del efecto isotopico, en

  20. EL PROCESO DE FORMACIÓN PROFESIONAL DESDE UN PUNTO DE VISTA COMPLEJO E HISTÓRICO-CULTURAL

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    Odalia Llerena Companioni

    2015-01-01

    Full Text Available En este ensayo se valoran conceptos e ideas centrales en la obra de Lev. S. Vigotsky y Edgar Morin y se expresan algunos puntos de enlace entre ellas que pueden servir de base al reconocimiento de la formación profesional como proceso complejo, a partir de la Teoría de la Complejidad y el Enfoque Histórico-Cultural como bases teóricas-metodológicas que permiten valorar el desarrollo del proceso de formación profesional, desde una perspectiva crítica, integral y contextualizada culturalmente. Los puntos de referencia que tradicionalmente, caracterizaron el análisis del proceso de formación son objeto de significativas modificaciones en lo que puede denominarse la era de la educación global. Dichos cambios provienen tanto de la evolución científica y tecnológica, como de una concepción educativa que pone en el centro el desarrollo integral del ser humano. Para responder a esta realidad se precisa observar dicho proceso de formación a partir de las peculiaridades individuales y las condiciones en que este se produce, así como las relaciones de mutua influencia entre todos estos aspectos que le otorgan un carácter complejo.

  1. Simulaciones hidrodinámicas de flujos complejos

    Science.gov (United States)

    María Ibáñez Cabanell, José

    Son muchos los escenarios astrofísicos en que los procesos hidrodinámicos juegan un papel clave. En la complejidad que encierra la descripción de dichos procesos destaca el de la correcta simulación de flujos complejos donde la presencia de ondas de choque fuertes que, eventualmente, interaccionan entre ellas o también la presencia de inestabilidades (Kelvin-Helmholtz, Rayleigh-Taylor, etc.) suponen un verdadero desafío numérico. Los códigos hidrodinámicos basados en la solución de un problema de valores iniciales discontinuo (problema de Riemann) son, en la actualidad, los más robustos en el campo de la dinámica de fluidos computacional. En esta charla se dará un resumen de los fundamentos de dichas técnicas numéricas (esquemas de alta resolución de captura de choques) y se ilustrará su potencialidad mostrando una amplia gama de resultados en diferentes aplicaciones astrofísicas.

  2. Complejo parroquial de Santa Ana, en Moratalaz, Madrid

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    Fisac, Miguel

    1967-06-01

    Full Text Available This church is sited at Moratalaz, a modern district of low rent houses in Madrid, and the architect has attempted to represent in the design the liturgical concepts developed in the Vatican II Council. These precise ideas have been expressed in the architecture by means of highly plastic symbols. In addition, the planform is very functional and the reinforced and prestressed concrete have been almost the only material used: so that the construction medium is also in accord with the modern nature of the design. The shapes, the space arrangement and everything which might seem a capricious creation of the artist in fact are the result of a detailed analysis of function and reason.Este complejo parroquial se ha levantado en Moratalaz, que es un barrio popular de la capital de España, procurando imprimir y recoger todas las premisas litúrgicas marcadas por el Concilio Vaticano II. El arquitecto ha plasmado sus precisas ideas en un edificio de gran expresividad plástica y de planta orgánica y eficaz, utilizando, casi exclusivamente, un material de nuestro tiempo: hormigón armado y pretensado. Las formas, la disposición de espacios y todo cuanto puede parecer caprichosa creación del artista, no son, en realidad, sino resultado de un cuidadoso estudio de funcionalismo racional y utilitario.

  3. ESTUDIOS DE AISLAMIENTO Y FRACCIONAMIENTO DE UN COMPLEJO GLICOPROTEICO DE PASTEURELLA MULTOCIDA

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    Yolanda de Navarro

    2010-07-01

    Full Text Available Mediante la extracción con solución de NaCl 2.5% (p/v a 47' C, se obtuvo un complejo glicoproteíco de Pasteurella multocida que fue parcialmente purificado mediante filtración por gel usando Sephacryl S-200. Las fracciones 1, 2 y 3 presentaron líneas de precipitinas en imnunodifusión contra sueros hiperimnunes de conejos inoculados con extracto salino 2.5% (P/V a 47" C y 66' C. Por electroforesis SDS-PAGE. se determinaron bandas de proteínas con pesos moleculares entre 98.800 y 17.700 daltons. Lafracción lyel extracto salino crudo a 47" C se utilizaron como antígenos adsorbidos en gel de Al(OHj y con ellos se efectuaron ensayos de protección en ratones, teniendo como referencia la vacuna comercial contra Septicemia Hemorrágica producida por VECOL S.A. Mediante el método estadístico de Reed Muench se estableció el índice de protección y se encontró que todos los antígenos fueron considerados prolectores, siendo la fracción 1 la de mayor índice de protección con una dosis inferior.

  4. Introducción a un modelo complejo de los softwares multimediales educativos

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    Luis R. Lara

    2004-01-01

    Full Text Available El diseño de softwares multimediales educativos como materiales para educación a distancia, ha llevado a que éstos sean realizados basados en principios de desarrollos empíricos. Este artículo, desde una nueva perspectiva teórica interdisciplinaria, enfoca la cuestión del desarrollo de estos materiales interactivos, considerándolos como Sistemas Interactivos Multimediales (SIM, relacionándolos con principios de disciplinas y conceptos tan diversos como la termodinámica, entropía, constructivismo y teoría general de sistemas. De esta manera, se orienta el proceso de diseño de los SIMs como un sistema complejo aceptando, desde una perspectiva constructivista, que el alumno toma el rol protagónico en el proceso de aprendizaje, entonces, un nuevo modelo de software multimedial educativo debería implicar al alumno no sólo en el tramo final como usuario, sino que tendría que intervenir durante toda la etapa en que se va realizando el proceso de aprendizaje.

  5. El complejo cárnico uruguayo ante la realidad del MERCOSUR

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    Segrelles Serrano, José Antonio

    2002-03-01

    Full Text Available The agricultural origins of Uruguay are still a highly important factor in its present economy, although meat agrocomplex has a special weight concerning both production and foreign trade. The Uruguay meat agrocomplex will have to face progressive globalization of the economy and growing free trade around the world. Regional integration in Mercosur" will be an efficient instrument to entrance the comparative advantages of the different livestock subsectors. However, we should not forget the repercussions that proceed from economic power, competitivity and political, monetary and financial stability of Argentina and Brazil.

    [es] Los orígenes agropecuarios de Uruguay continúan representando una realidad trascendental en su economía actual, aunque ante todo destaca el complejo cárnico, tanto por lo que respecta a la producción como al comercio exterior. El complejo cárnico uruguayo va a tener que hacer frente a la progresiva globalización de la economía y a la creciente liberalización comercial en el mundo. Su integración regional en el Mercosur puede ser un instrumento eficaz para resaltar las ventajas comparativas de sus distintos subsectores ganaderos. Sin embargo, no conviene olvidar las repercusiones que deriven del peso económico, posible competencia y estabilidad política, monetaria y financiera de Argentina y Brasil. [fr] Les origines du complexe agricole et de l'élevage représente encore aujourd'hui une réalité vitale pour son économia actuelle même si c'est surtout l'industrie de la vianda celle qui se détache aussi bien par sa production que par son commerce extérieur. Les industries de la viande à Uruguay doit affronter la progressive globalisation de l'économie et la libéralisation commerciale toujours croissante dans le monde. Leur intégration régionale dans Mercosur peut être un instrument efficace pour la mi se eu relief des avantages des différents sous-secteurs de l'élevage et la comparaison

  6. Las ignimbritas del complejo volcánico Coranzuli (Puna Argentina-Andes Centrales

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    Martí, J.

    1987-12-01

    Full Text Available The Coranzulí is a large Upper Miocene volcanic complex located on a NE-SW and NW-SE regional faults intersection, at 66º 15' W 23º S, in the Northwest of Argentina in Jujuy province. It corresponds to one of four large volcanic complexes which represent the end of Transversal Volcanic Chaines in La Puna Argentina in the Central Andes. The volcanic activity was characterized by at least four separate ignimbrite eruptions which produced four different ignimbrite sheets. These are well welded, very crystal-rich, lithie poor ignimbrites and have a moderate to high pumice content. The total volume of the Coranzuli ignimbrites exeededs 650 Km3. Preliminary data indicate that the eruption oeeurred from a homogeneous magmatic chamber without zoning. The emplacement characteristics of the ignimbrites and the lack of basal or interbedded plinian fall deposits suggest that the eruptions developed quickly to massive proportions.El Coranzuli es uno de los grandes complejos volcánicos que representan el remate final de las Cadenas Volcánicas Transversales de la Puna Argentina, en los Andes Centrales. Se trata de un complejo volcánico del Mioceno superior situado a los 66º 15' W 23º S en el NW de Argentina en la provincia de Jujuy, en la intersección entre dos fallas regionales de dirección NE-SW y NW-SE, respectivamente. La actividad eruptiva se caracterizó por la existencia de, al menos, cuatro erupciones ignimbríticas que produjeron cuatro diferentes mantos ignimbríticos. Se trata de ignimbritas bien soldadas, muy ricas en cristales, pobre en fragmentos líticos y que presentan un contenido en fragmentos pumíticos de moderado a alto. El volumen total que representan estas ignimbritas supera los 650 km3. Los datos preliminares indican que el magma juvenil deriva de una cámara magmática homogénea no zonada. Las características de emplazamiento de estas ignimbritas, así como la falta de depósitos plinianos de caída en la base o

  7. Resultados del tratamiento quirúrgico de los aneurismas del complejo cerebral anterior-arteria comunicante anterior

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    Armando Alemán Rivera

    2001-06-01

    Full Text Available Se realiza un estudio de 30 pacientes con aneurismas localizados en el complejo de la arteria cerebral anterior-arteria comunicante anterior (ACoA, operados en el Servicio de Neurocirugía del Hospital Universitario "Arnaldo Milián Castro", durante un período de 7 años. Se analizan variables tales como edad, sexo, estado neurológico preoperatorio, momento quirúrgico, complicaciones y estado al egreso. La mortalidad general fue del 10 %The authors carried out a study in 30 patients with aneurysms located in the anterior communicating artery-anterior cerebral complex (ACA-ACC, that were operated on at the Neurosurgery Service of "Arnaldo Milián Castro" Teaching Hospital, during a period of 7 years. Variables such as age, sex, preoperative neurologic state, surgical moment, complications and status on discharge were analyzed. General mortality was 10 %

  8. Estimating large complex projects Estimando proyectos grandes y complejos

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    Cliff Schexnayder

    2007-08-01

    Full Text Available Managing large capital construction projects requires the coordination of a multitude of human, organizational, technical, and natural resources. Quite often, the engineering and construction complexities of such projects are overshadowed by economic, societal, and political challenges. The ramifications and effects, which result from differences between early project cost estimates and the bid price or the final project cost, are significant. Over the time span between the initiation of a project and the completion of construction many factors influence a project's final costs. This time span is normally several years in duration but for highly complex and technologically challenging projects, project duration can easily exceed a decade. Over that period, changes to the project scope often occur. The subject here is a presentation of strategies that support realistic cost estimating. Through literature review and interviews with transportation agencies in the U.S. and internationally the authors developed a database of the factors that are the root causes of cost estimation problemsGestionar proyectos de construcción de grandes capitales requiere de la coordinación de una multitud de recursos humanos, organizacionales, técnicos y naturales. Frecuentemente, las complejidades del diseño y construcción de esos grandes proyectos son tapadas por sus desafíos económicos, políticos y sociales. Las ramificaciones y efectos que resultan de las diferencias entre la estimación de costo inicial, el costo de la propuesta adjudicada y el costo final del proyecto son significativas. Hay numerosos factores que inciden en el costo final del proyecto entre su inicio y finalización. La duración es generalmente de varios años y puede incluso superar la década para aquellos especialmente complejos y desafiantes. En ese período de tiempo, cambios en los alcances del proyecto cambian frecuentemente. El tópico del presente artículo es mostrar

  9. Valoración del dolor en neonatos: propuesta de un modelo de registro en la unidad de neonatología del complejo hospitalario de Navarra

    OpenAIRE

    Preciado Erro, Amaia

    2013-01-01

    En las unidades de cuidados intensivos de neonatología se expone a los pacientes a un número alto de procedimientos dolorosos que afectan al estado de consciencia y el estado fisiológico de los niños. Este trabajo de fin de grado tiene como objetivo la propuesta de un modelo de registro tanto de la valoración como del manejo del dolor en recién nacidos en la unidad de neonatología del Complejo Hospitalario de Navarra (B), que facilite la monitorización de este como un quinto signo vital m...

  10. Complejo «Australia Square», en Sidney – Australia

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    Seidler, Harry

    1971-02-01

    Full Text Available This project has given a new style to the overcrowded centre of Sydney city. Its outstanding feature is the 50 storey, 170.70 m high tower, of circular cross section, which is a distinctive feature in the urban skyline. Most of the building is devoted to office space, but the lower floors include a commercial centre, and a theatre, which can be converted into a convention hall. At the top there is a terrace, offering a fine view of the town, and just below a rotating restaurant, with capacity for 330 guests, which turns round once every ninety minutes and enables diners to enjoy a total view of Sydney in the course of a meal. This building is also significant because of its structural design, the good space distribution, and the novelty and quality of its internal fittings.Destaca en este complejo, que ha aportado un nuevo sentido de vida al congestionado centro de Sydney, la torre de 50 plantas, 170,69 m de altura, y planta circular, que constituye un hito destacado en el centro de la ciudad. La mayor parte de sus plantas están destinadas a oficinas; las inferiores se dedican a centro comercial, y albergan un teatro convertible en sala de Juntas y Reuniones. La planta superior incluye una terraza, desde la que los visitantes pueden disfrutar de la vista de Sydney; debajo de ella hay un restaurante giratorio, con capacidad para 330 plazas, desde el cual los comensales pueden contemplar una vista panorámica completa del área metropolitana de Sydney, ya que su plataforma da una vuelta completa cada hora y media. Características de este edificio son: la bella solución estructural que presenta; máximo aprovechamiento del espacio logrado; flexibilidad en la distribución, y perfección y originalidad de sus instalaciones.

  11. Sistema de reconocimiento y diagnóstico de arritmias cardíacas aplicado a la identificación de taquicardias de complejos anchos a partir del ECG

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    Oscar Javier Olarte

    2009-01-01

    Full Text Available Se presenta una herramienta software como soporte al diagnóstico diferencial de taquicardias de complejos anchos basado en la metodología clínica de diagnóstico Bayesiano. El sistema consta de módulos independientes para: a Detección de complejos, con sensibilidad (Sn de 94% y valor predictivo positivo (VP+ de 97,5%. b cómputo de la duración del complejo, con error cuadrático medio (ERMS de 25,2 ms c determinación del eje eléctrico del complejo QRS, con error RMS de 5,89º d determinación de morfología de bloqueo de rama, con Sn 89,9% y VP+ 93,3% y e clasificación de morfologías de QRS, donde se obtuvo clasificación completa. La técnica empleada se basa en detección de cruces por cero y valores singulares (máximos y mínimos en la transformada wavelet. Por su parte, el sistema de clasificación de morfologías se desarrolla con redes neuronales artificiales.

  12. Genotipificación de aislamientos clínicos del complejo Cryptococcus neoformans/Cryptococcus gattii obtenidos en el Hospital «Dr. Julio C. Perrando», de la ciudad de Resistencia (Chaco, Argentina

    Directory of Open Access Journals (Sweden)

    Maria E Cattana

    Full Text Available La criptococosis es una infección fúngica causada por levaduras del género Cryptococcus, particularmente las del complejo Cryptococcus neoformans/Cryptococcus gattii. El conocimiento sobre la casuística de la criptococosis en el nordeste argentino es exiguo y no se tiene información sobre los tipos moleculares circulantes. El objetivo de este estudio fue realizar la caracterización genética de los aislamientos pertenecientes al complejo C. neoformans/C. gattii obtenidos en el Hospital «Dr. Julio C. Perrando» de la ciudad de Resistencia (Chaco, Argentina, con el fin de determinar especie, variedad y genotipo. Durante dos años y un mes se estudiaron 26 aislamientos clínicos. Mediante métodos convencionales y moleculares, un aislamiento fue identificado como C. gattii genotipo VGI y los 25 restantes como C. neoformans var.grubii, 23 de los cuales correspondieron al genotipo VNI y dos al genotipo VNII. Estos datos son una contribución al conocimiento de la epidemiología de la criptococosis en la Argentina y el primer informe sobre genotipos del complejo C. neoformans/C. gattii de origen clínico en el nordeste argentino.

  13. Influencia del uso de complejos enzimáticos en la segunda centrifugación de pastas de aceitunas

    Directory of Open Access Journals (Sweden)

    Jackisch, Björn Oliver

    2006-09-01

    Full Text Available Virgin olive oil is a natural fruit juice that conserves the flavor, aroma, vitamins and all the properties of the fruit from which it comes, being, in addition, the only vegetal oil that can be consumed as “virgin” or completely unrefined. The extraction of the juice from the olive is carried out exclusively by mechanical means in the first centrifugation step, in order to obtain directly consumable virgin olive oil. The use of enzymatic complexes in the process of a second centrifugation step has been contemplated in order to obtain a greater yield of refinable virgin oil.With this work, we attempt to demonstrate an increase in the yield of oil extraction with the use of an enzymatic complex in the second centrifugation and to confirm that its use does not affect the quality of the oils obtainedEl aceite de Oliva Virgen es un zumo de fruta natural que conserva el paladar, perfume, vitaminas y todas las propiedades del fruto del que procede, siendo, además, el único aceite vegetal que puede consumirse directamente virgen y crudo. Se defiende la elaboración del zumo de aceituna por medios exclusivamente mecánicos en la primera centrifugación, para obtener aceites de oliva vírgenes directamente consumibles, pero se contempla el uso de complejos enzimáticos en los procesos de segunda centrifugación, para incrementar los rendimientos en la obtención de aceites vírgenes refinables.Pretendemos demostrar con este trabajo que el uso de un complejo enzimático en la segunda centrifugación aumenta el rendimiento del aceite y, al mismo tiempo, confirmar que su uso no tiene efecto en los parámetros de calidad de los aceites obtenidos

  14. Flotación en columnas de complejos cobre - amilxantato en función del pH

    Directory of Open Access Journals (Sweden)

    Francisco J. Tavera-Miranda

    2009-03-01

    Full Text Available La diferencia de solubilidad de los complejos amilxantato de cobre(I y de cobre(II es un elemento determinante en la implementación de procesos de flotación para recuperar cobre disuelto, aspecto en el que no se ha profundizado. En este trabajo se estudia el comportamiento de ambos complejos en función del pH del medio, evaluando los resultados a partir de su contribución específica en la recuperación y la cinética del proceso de flotación, el cual se lleva a cabo en una columna de laboratorio utilizando amilxantato de potasio como colector. El rango de pH utilizado (4 a 13 permite evaluar el efecto de las transformaciones que tienen lugar en el sistema amilxantato- cobre-agua. Los resultados indican el incremento de la recuperación de cobre con el incremento del pH del medio hasta valores de pH 11 y muestran una fuerte dependencia de la recuperación con las características químicas del sistema, el cobre flotado se incrementa marcadamente al incrementarse la concentración de la especie cristalina amilxantato de cobre(I, asociado a esto se manifiesta un incremento de la fracción de gas retenido y el flujo de superficie de las burbujas, las cuales tienen una incidencia directa sobre la cinética del proceso de flotación.

  15. Nuevos datos sobre la geologia del Complejo Intrusivo Alcalino de Monchique (SW de Portugal)

    OpenAIRE

    Valadares, V.; González Clavijo, Emilio J.

    2004-01-01

    Los estudios microscópicos complementarios de las rocas alcalinas han permitido confirmar la estructura anular groseramente concéntrica del Complejo de Monchique, semejante a la que presentan los complejos alcalinos coetáneos de Sintra y Sines. La zona central está constituida por sienitas nefelínicas de grano grueso, rodeadas por un anillo de sienitas nefelínicas heterogéneas de grano más fino. En esta unidad exterior, y localmente separando ambas unidades, existen cuerpos menores de rocas b...

  16. El complejo mayor de histocompatibilidad humano: sistema HLA

    Directory of Open Access Journals (Sweden)

    Luis Fernando García

    1989-02-01

    Full Text Available

    El complejo mayor de histocompatibilidad humano, o sistema HLA, está localizado en el brazo corto del cromosoma 6. Sus genes codifican tres tipos de moléculas. Los antígenos clase I (HLA-A, B, C y E están formados por una cadena pesada unida no covalentemente a la β2-microglobulina y se expresan en la superficie de la mayoría de las células nucleadas del organismo. Estos antígenos actúan como elementos de restricción en la activación de los linfocitos T CD8+. Los antígenos clase II son dímeros compuestos por cadenas α y β y su distribución tisular está limitada sólo a algunos tipos de células. Estas moléculas actúan restringiendo la presentación de antígenos a los linfocitos CD4+. Los antígenos de clase III son proteínas plasmáticas del sistema del complemento. Los diferentes loci del sistema HLA son muy polimórficos y sus productos se heredan en bloques conocidos como haplotipos. Debido a que los diferentes grupos étnicos presentan variaciones en la frecuencia de ale ios y haplotipos, el HLA ha sido muy útil en los estudios antropogenéticos. Algunos antígenos HLA están presentes en pacientes con determinadas enfermedades con una frecuencia significativamente diferente a la encontrada en la población general. Estos hallazgos han sido de gran importancia para comprender la patogénesis y los mecanismos genéticos de resistencia o susceptibilidad a dichas enfermedades. En el campo de los transplantes de órganos, la compatibilidad HLA donante-receptor correlaciona con la sobrevida del injerto. El sistema HLA también parece tener mucha importancia en los fenómenos inmunológicos que ocurren durante el

  17. In vitro Inhibition of Mycobacterium smegmatis and Mycobacterium ...

    African Journals Online (AJOL)

    Some Nigerian plants used in traditional medicine to treat tuberculosis and/or some of its symptoms were screened for in vitro activity against Mycobacterium smegmatis and a clinical isolate of Mycobacterium tuberculosis. Only 3 of the 6 crude methanolic extracts of the 6 plant species exhibited inhibitory activities against ...

  18. Filogeografía del complejo Caesalpinia hintonii: (Leguminosae: Caesalpinioideae: Poincianella Phylogeography of the Caesalpinia hintonii complex (Leguminosae: Caesalpinioideae: Poincianella

    Directory of Open Access Journals (Sweden)

    Solange Sotuyo

    2010-12-01

    Full Text Available A pesar del crecido número de estudios que se ha realizado para explorar la estructura genética y filogeográfica en especies mexicanas, hay carencia de estudios para especies de selva baja, zonas con alto grado de endemismo. Para un mejor entendimiento de dichas áreas se realizó un estudio filogeográfico y el fechamiento de las especies del complejo Caesalpinia hintonii distribuido en la depresión del río Balsas y el valle de Tehuacán-Cuicatlán. Se determinó la estructura filogeográfica del complejo analizando las secuencias de 4 regiones de plastidio (intrón trnL, espaciador trnL-F, espaciador psbA-trnH y espaciador accD-psaI en todas las poblaciones disponibles. Se estimó la fecha del origen del complejo mediante un método no paramétrico (NPRS. Las diversidades haplotídicas (0.64-1 y las diferencias nucleotídicas (3.8-11.52 en todas las especies fueron altas respecto a valores registrados para otras plantas. Las poblaciones de C. oyamae, morfológicamente indistintas están genéticamente estructuradas y presentan 3 haplogrupos donde el flujo génico entre ellos es bajo y se consideraron entidades crípticas. Las poblaciones de C. hintonii son un grupo genéticamente homogéneo a pesar de ser morfológicamente distintas. Las poblaciones de C. macvaughii forman 2 linajes divergentes entre los que existe flujo génico (Nm=1.41. Los eventos de divergencia en 2 grandes linajes al este y oeste del río Balsas pueden explicarse por eventos geológicos. Los resultados indican que el complejo se encuentra bajo divergencia y en algunos casos radiación morfostática.Although many phylogeographic studies have been conducted to analyze the genetic and phylogeographic structure of Mexican species, such studies are nearly absent for plants of dry seasonal forest, precincts with high level of endemism. To better understand this areas, we undertook a phylogeographic study and dating were carried out on the Caesalpinia hintonii complex

  19. Complejo de oficinas en Serrería

    OpenAIRE

    PÉREZ SANZ, LORENA

    2016-01-01

    El tema trabajado en este proyecto final de carrera es un edificio de oficinas y usos múltiples situado en la zona del Cabañal, en Valencia. El proyecto abarca el ámbito arquitectónico del propio proyecto y el urbanístico de la parcela donde se sitúa. El programa del edificio es bastante amplio. Las oficinas constituyen la mayor parte de la superficie construida. Estas están complementadas con usos de un carácter más público, tales como salas de exposiciones, sala de conferencias, salas ...

  20. Adipocinas y síndrome metabólico: múltiples facetas de un proceso fisiopatológico complejo

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    Julio C. Sánchez N., MD., PhD

    2010-07-01

    Full Text Available Debido a la alta morbimortalidad de las enfermedades cardiovasculares y a su relación con trastornos de base como la obesidad y el síndrome metabólico, es crucial entender cuáles son los mecanismos y procesos que desencadenan la alteración del metabolismo y a su vez la generación de dichas enfermedades. En tal sentido, el tejido adiposo y el adipocito tienen un papel fundamental en este proceso, mediante la producción de múltiples adipocinas, algunas clásicas y otras de reciente descripción, pero que hasta ahora empieza a dilucidarse en medio del complejo panorama de interacciones fisiopatológicas conducentes al desarrollo de resistencia a la insulina y del complejo desequilibrio metabólico que conlleva un sinnúmero de complicaciones clínicas. Un grupo de estas adipocinas tiene claros efectos proinflamatorios, mientras que otras pueden clasificarse como anti-inflamatorias, las cuales contrarrestan en cierta medida y hasta cierto punto las acciones de las otras. Cuando esta homeostasis se rompe, la cascada de inflamación crónica allí originada desencadena resistencia a la insulina y se inicia el desarrollo del síndrome metabólico a partir de la obesidad, que a su vez genera alteraciones de la respuesta del adipocito a diferentes estímulos. Esto, sumado a los efectos de otros elementos, configura un complejo cuadro de factores que es necesario tener en cuenta para el abordaje correcto de la obesidad y sus patologías asociadas.

  1. Complejos de Cu(II: Alternativas en la terapia antineoplásica Complejos de Cu(II: Alternativas en la terapia

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    Myrna Sabanero López

    2012-02-01

    Full Text Available En el presente estudio, fué evaluada la actividad biológica de nuevos complejos de cobre (II fenantrolina unidos a aminoácidos, como ácido glutámico, isoleucina y α-metil-dopa. Para ésto se utilizaron células neoplásicas de pulmón humano y dos dosis (30 y 60 μg/ml de los compuestos fueron aplicadas a los cultivos celulares. También se realizaron ensayos inmunocitoquímicos para microtúbulos, microfi lamentos y electroforesis del DNA genómico. Los resultados obtenidos muestran que los complejos de Cu (II fenantrolina con ácido glutámico y α-metil-dopa afectan la adhesión celular (50% y 45%, respectivamente, alterando la distribución de los microtúbulos y microfi lamentos. La fragmentación del DNA, apoya el ensayo del efecto antitumoral del Cu (II fenantrolina con ácido glutámico y α-metil-dopa sobre los cultivos de células de pulmón humano in vitro, de manera dosis dependiente. En conclusión, los complejos de Cu (II fenantrolina con ácido glutámico y α-metil-dopa, presentan un efecto signifi cativo sobre la actividad celular, sugiriendo que estos complejos podrían ser potencialmente compuestos antitumorales. In this study, the biological activity of new copper (II phenanthroline compounds bound to amino acids such as glutamicacid, isoleucine and α-methyl dopa was evaluated. To accomplish this, tumor cells from the human lung and two doses of compounds (30 and 60 μg/ml were applied to cell cultures. Also, immunostaining of microtubules, phalloidin staining of microfilaments, and electrophoresis of genomic DNA were performed. The results showed that compounds of Cu(II phenanthroline with glutamic acid and α methyl-dopa significantly affected cellular adhesion (by 50% and 45%, respectively by altering the distribution of microtubules and microfilaments. Evaluation of DNA fragmentation supported the tumor suppressing effect of Cu (II Phenantroline with glutamicacid and α-Methyl Dopa on human lung cell cultures in

  2. Representaciones de un mundo complejo: No tinc paraules de Arnal Ballester

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    Estrella Sánchez Marcos

    2010-03-01

    Full Text Available En este artículo se analiza cómo en los libros destinados a niños actúan dos factores, el supuesto de simplicidad y el doble destinatario. En el primero se presupone que el niño no tiene la madurez intelectual de un adulto y por tanto los temas, motivos y representaciones deben ser más simples. No obstante, en los estudios sobre formas de lectura y recepción, los niños se revelan como lectores críticos y sofisticados. El doble destinatario es el niño más el adulto que habitualmente en primeras edades lee el libro con él. Este adulto pasará a un primer plano en ese proceso de comunicación que se establece entre un autor y sus dos destinatarios. A su vez, la industria editorial complace con libros a la carta a ese adulto que selecciona, elige y compra. Todos estos factores colisionan con la necesidad del niño para formar su imaginario simbólico con historias y representaciones complejas. Se plantea así cómo los autores-ilustradores actuales de libros para niños pueden hacer visibles o no esos niveles de profundidad y encontrar en formatos como el álbum ilustrado, vehículos para mostrar significados complejos. Se estudia en detalle No tinc paraules, un libro de Arnal Ballester donde el ilustrador elige motivos como el erotismo, el amor, la reflexión sobre la identidad; convierte al niño en el destinatario principal de una historia que tiene que construir él mismo, y deja que el adulto le acompañe. A través de él se puede demostrar que la experimentación gráfica y las ideas pueden contenerse en el mismo espacio.

  3. La teoría del granulado y su aplicación a sistemas moleculares complejos

    OpenAIRE

    Hijón de Miguel, Carmen

    2009-01-01

    El objetivo de esta tesis es el desarrollo de modelos de grano grueso para la simulación de sistemas moleculares complejos. Entendemos que un modelo de grano grueso es aquel que representa un sistema físico con menor número de grados de libertad que los que hay realmente en el sistema. Y por sistema molecular complejo entendemos aquel cuyos constituyentes son macromoléculas. El reto que plantea la simulación de los sistemas moleculares complejos es el desarrollo de modelos capaces de describi...

  4. Pensamiento complejo y estudio de la comunicación

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    Mapi Ballesteros Panizo

    2013-03-01

    Full Text Available En aquest article es pretén enumerar una sèrie de reptes teòrics i metodològics que les ciències que estudien la comunicació tenen al davant. Partiré del pensament complex de Morin, perquè a la seva obra he trobat un diagnòstic molt agut de la situació que travessen les ciències socials i pistes suficients per millorar-la. D'acord amb aquest objectiu, he dividit la meva exposició en quatre parts: Primerament, tractaré de resumir què és el pensament complex i em detindré en l'explicació d'un concepte fonamental en aquesta perspectiva: el d'emergència. En el segon apartat examinaré dos àmbits que a l'estudiós de la comunicació se li obren si adopta una mirada complexa. A continuació, presentaré dos dels principis que regeixen aquest tipus d'aproximació: el principi de recursivitat organitzacional i el principi hologramàtic. En l'última part del treball proposaré distingir entre un tipus d'emergència física i una altra humana. Penso que, encara que fins avui ningú s’'hagi fet, es tracta d'un pas imprescindible per a l'estudi de l'acció social. Reconèixer les peculiaritats de l'emergència que es dóna en els sistemes personals té importants repercussions metodològiques. Acabaré fent un breu resum de les idees desenvolupades.

  5. Mycobacterium persicum sp. nov., a novel species closely related to Mycobacterium kansasii and Mycobacterium gastri.

    Science.gov (United States)

    Shahraki, Abdolrazagh Hashemi; Trovato, Alberto; Mirsaeidi, Mehdi; Borroni, Emanuele; Heidarieh, Parvin; Hashemzadeh, Mohamad; Shahbazi, Narges; Cirillo, Daniela M; Tortoli, Enrico

    2017-06-01

    Four strains isolated in Iran from pulmonary specimens of unrelated patients are proposed as representative of a novel Mycobacterium species. Similarity, at the phenotypic level, with Mycobacterium kansasii is remarkable with the photochromogenic yellow pigmentation of the colonies being the salient feature. They differ, however, genotypically from this species and present unique sequences in 16S rRNA, hsp65 and rpoB genes. The average nucleotide identity and the genome-to-genome distance fully support the status of an independent species. The name proposed for this species is Mycobacterium persicum sp. nov. with AFPC-000227T (=DSM 104278T=CIP 111197T) as the type strain.

  6. EL PENSAMIENTO COMPLEJO Y LA TRANSDISCIPLINARIEDAD: FENÓMENOS EMERGENTES DE UNA NUEVA RACIONALIDAD

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    SERGIO NÉSTOR OSORIO GARCÍA

    2012-01-01

    Full Text Available Hoy tenemos que aprender a diferenciar y a distinguir, sin tener por ello que separar. En este sentido hay dos pares de conceptos claramente diferenciables y no por ello separables: por un lado, los conceptos complejidad y pensamiento complejo y por otro lado, conocimiento disciplinar (multi-poli e interdisciplinar y conocimiento transdisciplinar. Para la ciencia clásica el pensamiento complejo y el conocimiento transdisciplinar es un absurdo, pero para éstos, el ideal de racionalidad de la ciencia clásica es necesario y válido, pero insuficiente para comprender la Realidad en el tiempo actual. Se hace necesario un nuevo paradigma de racionalidad que permita pensar la unidad de los conocimientos fragmentados en disciplinas de cara a la supervivencia de la especie humana en esta era que se ha convertido en planetaria. La presente reflexión busca presentar desde las apuestas conceptuales de Edgar Morin (pensamiento complejo y de Basarab Nicolescu (conocimiento transdisciplinar la dimensión epistemológica, ontológica y metodológica de la emergencia de esta nueva racionalidad.

  7. Diversidad alfa y beta de la comunidad de reptiles en el complejo cenagoso de Zapatosa, Colombia

    Directory of Open Access Journals (Sweden)

    Guido Fabián Medina-Rangel

    2011-06-01

    Full Text Available La diversidad es una propiedad de las comunidades, permite describir, caracterizar y entender el funcionamiento de los ecosistemas. Para estudiar la riqueza, abundancia y el recambio de especies de reptiles entre hábitats circundantes al complejo cenagoso de Zapatosa, se realizaron cuatro salidas de campo (noviembre de 2006 y octubre de 2007. Se invirtieron 640h/hombre de muestreo repartido en cinco tipos de hábitat: bosque casmófito, bosque seco, bosque de ribera, palmar y sabana arbolada. Se encontraron 847 individuos de 48 especies, la familia más rica fue Colubridae, se registraron cinco especies endémicas y ocho con algún grado de amenaza en conservación a nivel nacional. El hábitat más diverso fue el bosque de ribera. La representatividad del muestreo en total y para cada hábitat fue superior al 80%, con excepción del palmar (Alfa and beta diversity of reptilian assemblages in Zapatosa wetland complex, Colombia. Diversity is a property of community that can described, characterized, and understood according to the functioning of ecosystems. To study the richness and local abundance and species replacement between habitats around the Zapatosa’s wetland complex (El Cesar Department, I carried out four field trips between November of 2006 and October of 2007. A total of 640 sampling hours/man analyzed five habitat types chasmophyte forest, dry forest, riparian forest, palm-grove and tree-lined savanna; with the exception of the palm-grove sampled at its 75%, the others were sampled up to their 80%. I found 847 reptiles that were distributed in 48 species. The group with the highest number of species was Colubridae with 14, followed by Gekkonidae with five. Five endemic species and eight with some conservation threat grade at a national level a re reported. The riparian forest was the richest and most abundant habitat with 34 species and 196 individuals. For each habitat, Colubridae had the highest number of species, followed by

  8. Mycobacterium ulcerans disease, Peru.

    Science.gov (United States)

    Guerra, Humberto; Palomino, Juan Carlos; Falconí, Eduardo; Bravo, Francisco; Donaires, Ninoska; Van Marck, Eric; Portaels, Françoise

    2008-03-01

    Eight adult patients (ages 18-58, 5 women) with Buruli ulcer (BU) confirmed by at least 2 diagnostic methods were seen in a 10-year period. Attempts to culture Mycobacterium ulcerans failed. Five patients came from jungle areas, and 3 from the swampy northern coast of Peru. The patients had 1-5 lesions, most of which were on the lower extremities. One patient had 5 clustered gluteal lesions; another patient had 2 lesions on a finger. Three patients were lost to follow-up. All 5 remaining patients had moderate disease. Diverse treatments (antituberculous drugs, World Health Organization [WHO] recommended antimicrobial drug treatment for BU, and for 3 patients, excision surgery) were successful. Only 1 patient (patient 7) received the specific drug treatment recommended by WHO. BU is endemic in Peru, although apparently infrequent. Education of populations and training of health workers are first needed to evaluate and understand the full extent of BU in Peru.

  9. Mycobacterium tuberculosis Metabolism

    Science.gov (United States)

    Warner, Digby F.

    2015-01-01

    Metabolism underpins the physiology and pathogenesis of Mycobacterium tuberculosis. However, although experimental mycobacteriology has provided key insights into the metabolic pathways that are essential for survival and pathogenesis, determining the metabolic status of bacilli during different stages of infection and in different cellular compartments remains challenging. Recent advances—in particular, the development of systems biology tools such as metabolomics—have enabled key insights into the biochemical state of M. tuberculosis in experimental models of infection. In addition, their use to elucidate mechanisms of action of new and existing antituberculosis drugs is critical for the development of improved interventions to counter tuberculosis. This review provides a broad summary of mycobacterial metabolism, highlighting the adaptation of M. tuberculosis as specialist human pathogen, and discusses recent insights into the strategies used by the host and infecting bacillus to influence the outcomes of the host–pathogen interaction through modulation of metabolic functions. PMID:25502746

  10. Arquitectura y anatomía foliar del complejo Polypodium plesiosorum sensu Moran (Polypodiaceae

    Directory of Open Access Journals (Sweden)

    J. Daniel Tejero-Díez

    2010-09-01

    Full Text Available El complejo Polypodium plesisorum es un grupo de morfología heterogénea. Se estudió la arquitectura y anatomía foliar de 12 especies que constituyen el complejo de Polypodium plesiosorum reconocidas por Moran, y otras 23 especies de Polypodium (complejos P. dulce, P. polypodioides y P. vulgare sunsu Moran y géneros relacionados taxonómicamente (Goniophlebium, Phlebodium, Pleopeltis, Serpocaulon, Synammia con fines comparativos. Dos grupos de especies homogéneas fueron establecidos en el complejo P. plesisorum. Uno de ellos se reconoce por el tipo de anastomosis de las venas (tipo III: Polypodium conterminans (originalmente considerado en el grupo de P. dulce de venación libre según Moran, P. hispidulum, P. plesiosorum y P. rhodopleuron. El segundo grupo formado por Polypodium arcanum, P. castaneum, P. colpodes, P. eatonii y P. flagellare, se reconoce por el tipo de anastomosis de las venas (tipo IV y la forma en que se fusionan los cordones vasculares a lo largo del eje pecíoloraquis de la hoja. Los caracteres estudiados ayudaron a relacionar las especies restantes con otros grupos fuera del complejo estudiado.Architecture and leaf anatomy of the Polypodium plesiosorum sensu Moran complex (Polypodiaceae. The Polypodium plesisorum complex is a heterogeneous morphology group. We studied leaf architecture and anatomy of 12 species of this group, in addition to other 23 species of Polypodium (P. dulce, P. polypodioides and P. vulgare complex sensu Moran and related genera (Goniophlebium, Phlebodium, Pleopeltis, Serpocaulon, Synammia, for comparative purposes. Two homogeneous groups of species were established in P. plesiosorum complex. One of them is recognized based on the type of anastomosis of the veins (Type III: Polypodium conterminans (originally considered in the group of P. dulce with free venation by Moran, P. hispidulum, P. plesiosorum and P. rhodopleuron. The second group with Polypodium arcanum, P. castaneum, P. colpodes, P

  11. Mycobacterium chelonae y Mycobacterium abscessus: patógenos emergentes

    Directory of Open Access Journals (Sweden)

    Mónica M. Ortegón

    1996-09-01

    Full Text Available Mycobacterium chelonae es el nombre correcto para la micobacteria aislada en 1903 de los pulmones enfermos de una tortuga marina. En una especie distinta de Mycobacterium fo/tuitum, aislado de ranas en 1905, y de Mycobacterium abscessus, considerado actualmente como una subespecie de M chelonae. Estas tres especies son las únicas patógenas para el hombre dentro del grupo de micobacterias ambientales o atipicas, de crecimiento rápido, las cuales se caracterizan por formar colonias en cultivo en menos de siete días. Son agentes etiológicos de nódulos y abscesos cutáneos, localizados y diseminados, de lesiones postoperatorias, usualmente en la cicatriz quirúrgica, de lesiones pulmonares y de linfadenitis granulomatosa, de osteomielitis y de queratitis, entre otras. Las lesiones cutáneas y de los tejidos blandos son las más frecuentes y resultan generalmente de la inoculación traumática de esta micobacteria. Histopatológicamente, los nódulos y abscesos muestran un proceso inflamatorio, supurativo y granulomatoso, mixto, en el que en la cuarta parte de los casos pueden demostrarse conglomerados de bacilos ácido alcohol resistentes, que tienden a estar situados en una vacuola en el centro del absceso. En Colombia, se han descrito tres brotes de abscesos subcutáneos producidos por bacterias ambientales, secundarios a la aplicación de inyecciones contaminadas con el germen causal: en 1981, en Bucaramanga, luego de la aplicación de la vacuna contra la fiebre amarilla, en 50 personas, la mayoría niños; en 1989, en Medellin, por la inyección subcutánea de alergenos, en 13 personas; y, en 1993, en varias ciudades de la costa atlántica, luego de aplicaciones subcutáneas de xilocaína, como tratamiento bionergético, en 297 pacientes. Existen otros informes aislados de casos posttraumáticos.La enfermedad diseminada por micobacterias de rápido crecimiento, se presenta en pacientes inmunosuprimidos. En la biopsia, predominan los

  12. Diversity and evolution of drug resistance mechanisms in Mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Al-Saeedi M

    2017-10-01

    Full Text Available Mashael Al-Saeedi, Sahal Al-Hajoj Department of Infection and Immunity, Mycobacteriology Research Section, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia Abstract: Despite the efficacy of antibiotics to protect humankind against many deadly pathogens, such as Mycobacterium tuberculosis, nothing can prevent the emergence of drug-resistant strains. Several mechanisms facilitate drug resistance in M. tuberculosis including compensatory evolution, epistasis, clonal interference, cell wall integrity, efflux pumps, and target mimicry. In this study, we present recent findings relevant to these mechanisms, which can enable the discovery of new drug targets and subsequent development of novel drugs for treatment of drug-resistant M. tuberculosis. Keywords: Mycobacterium tuberculosis, antibiotic resistance, compensatory evolution, epistasis, efflux pumps, fitness cost

  13. Mycobacterium fortuitum causing surgical site wound infection

    International Nuclear Information System (INIS)

    Kaleem, F.; Usman, J.; Omair, M.; Din, R.U.; Hassan, A.

    2010-01-01

    Mycobacterium fortuitum, a rapidly growing mycobacterium, is ubiquitous in nature. The organism was considered to be a harmless saprophyte but now there have been several reports from different parts of the world wherein it has been incriminated in a variety of human infections. We report a culture positive case of surgical site infection caused by Mycobacterium fortuitum, who responded well to the treatment. (author)

  14. Propiedades insecticidas de la ceniza del complejo volcánico Puyehue-Cordón Caulle y su posible impacto ambiental

    Directory of Open Access Journals (Sweden)

    Micaela BUTELER

    2011-01-01

    Full Text Available Se examina el efecto insecticida de las cenizas volcánicas en general y sus efectos ambientales, con especial énfasis en las cenizas acumuladas como consecuencia de la erupción del complejo volcánico Puyehue-Cordón Caulle, que actualmente afecta un área de más de 7,5 millones de hectáreas en la Patagonia Argentina. Se determinó la composición química de las cenizas provenientes del complejo Puyehue- Cordón Caulle por EDS, y se comprobó que los componentes mayoritarios son el O, Si, Al, Fe, Na y K. A través de bioensayos de laboratorio, utilizando dos especies de insectos como modelo biológico, se determinó que las cenizas se adhieren profusamente a la superficie corporal y resultan tóxicas para ambas especies cuando se mezclan con el sustrato en baja concentración. La susceptibilidad a las cenizas difirió entre ambas especies en bioensayos de siete días de exposición continua [CL99(Oryzaephilus surinamensis L. = 10.25 mg.g-1; CL99((Tribolium castaneum (Herbst = 4.96 mg.g-1]. De estos resultados, se desprende que el impacto de la ceniza del complejo Puyehue- Cordón Caulle sobre la comunidad de artrópodos puede ser radical en la fase aguda de la deposición, y que sus efectos podrían ser substancialmente cualitativos en la fase crónica de la exposición. Así también, la recuperación de las poblaciones afectadas por la ceniza en las zonas con clima desértico, donde persiste la ceniza seca, será más lenta, hecho que tendrá consecuencias tangibles a largo plazo para las plantas hospederas de insectos, para la polinización y sobre el desarrollo de las poblaciones de vertebrados insectívoros. En contraste, en las regiones húmedas, la presencia de cenizas representa un cambio manifiesto en el ecosistema debido al aporte de nutrientes, que en combinación con la retención de agua por las cenizas, llevará a la recuperación cuali y cuantitativa de las poblaciones bajo condiciones favorables.

  15. Ambrona (Soria) : apuntes sobre la industria lítica del Complejo Superior

    OpenAIRE

    Rubio Jara, Susana

    1996-01-01

    La finalidad de este trabajo, resumen de mi tesis de licenciatura, es caracterizar tecnomorfológicamente la industria lítica del Complejo Superior de Ambrona, de la que hasta el momento sólo se ha publicado el número total de piezas pese haber sido objeto de estudio entre los años 60 y 80.This paper, summary of my gradúate thesis, characterizes in a technomorphological frame, the lithic industry of «The Upper Complex» in Ambrona site. This industry although it was exposing between the sixties...

  16. Ambrona (Soria : apuntes sobre la industria lítica del Complejo Superior

    Directory of Open Access Journals (Sweden)

    Susana Rubio Jara

    1996-01-01

    Full Text Available La finalidad de este trabajo, resumen de mi tesis de licenciatura, es caracterizar tecnomorfológicamente la industria lítica del Complejo Superior de Ambrona, de la que hasta el momento sólo se ha publicado el número total de piezas pese haber sido objeto de estudio entre los años 60 y 80.This paper, summary of my gradúate thesis, characterizes in a technomorphological frame, the lithic industry of «The Upper Complex» in Ambrona site. This industry although it was exposing between the sixties and eigthies, untll this moment it was unpublished.

  17. Suplementos nutricionales orales a base de nuevos complejos de cobre, magnesio, manganeso y zinc

    OpenAIRE

    Benavides Arévalo, Julie Fernanda; Tobón Zapata, Gloria Elena

    2011-01-01

    Suplementos nutricionales orales a base de nuevos complejos de cobre, magnesio, manganeso y zinc Los oligoelementos cobre, magnesio, manganeso y zinc intervienen en numerosos procesos metabólicos, enzimáticos, inmunológicos y tisulares, forman parte estructural de proteínas y pueden participar en la regulación de la expresión genética. La deficiencia de estos elementos esenciales dificulta el apropiado funcionamiento del organismo e induce el desarrollo de diversas enfermedades. Se debe garan...

  18. Modelado lingüístico de fenómenos complejos

    OpenAIRE

    Álvarez Álvarez, Alberto

    2012-01-01

    Actualmente, es posible adquirir y almacenar grandes volúmenes de datos sobre diferentes fenómenos complejos en muchas áreas importantes. Con el fin de ser útil, esta información debe ser explicada de la manera más comprensible posible, incluyendo los conocimientos previos disponibles sobre el fenómeno que se estudia. Estos objetivos sólo pueden lograrse mediante el uso de lenguaje natural, especialmente si la información final va a ser utilizada por personas no expertas. Por lo tanto, e...

  19. Plan de mantenimiento integral del Complejo de Castejón

    OpenAIRE

    Eslava Zaragüeta, Óscar

    2016-01-01

    Este proyecto de Fin de Grado está enfocado hacia el control y seguimiento del mantenimiento en el Complejo de Castejón, ya que en dicho establecimiento no hay ningún tipo de control ni un plan de mantenimiento establecido. Este hecho, provoca gastos económicos elevados ya que el mantenimiento que se realiza es un mantenimiento correctivo, es decir, cuando se rompe un equipo o componente, se arregla o se sustituye, lo que provoca un gasto mucho mayor, además de posibles in...

  20. Mycobacterium saopaulense sp. nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium chelonae--Mycobacterium abscessus group.

    Science.gov (United States)

    Nogueira, Christiane Lourenço; Whipps, Christopher M; Matsumoto, Cristianne Kayoko; Chimara, Erica; Droz, Sara; Tortoli, Enrico; de Freitas, Denise; Cnockaert, Margo; Palomino, Juan Carlos; Martin, Anandi; Vandamme, Peter; Leão, Sylvia Cardoso

    2015-12-01

    Five isolates of non-pigmented, rapidly growing mycobacteria were isolated from three patients and,in an earlier study, from zebrafish. Phenotypic and molecular tests confirmed that these isolates belong to the Mycobacterium chelonae-Mycobacterium abscessus group, but they could not be confidently assigned to any known species of this group. Phenotypic analysis and biochemical tests were not helpful for distinguishing these isolates from other members of the M. chelonae–M.abscessus group. The isolates presented higher drug resistance in comparison with other members of the group, showing susceptibility only to clarithromycin. The five isolates showed a unique PCR restriction analysis pattern of the hsp65 gene, 100 % similarity in 16S rRNA gene and hsp65 sequences and 1-2 nt differences in rpoB and internal transcribed spacer (ITS) sequences.Phylogenetic analysis of a concatenated dataset including 16S rRNA gene, hsp65, and rpoB sequences from type strains of more closely related species placed the five isolates together, as a distinct lineage from previously described species, suggesting a sister relationship to a group consisting of M. chelonae, Mycobacterium salmoniphilum, Mycobacterium franklinii and Mycobacterium immunogenum. DNA–DNA hybridization values .70 % confirmed that the five isolates belong to the same species, while values ,70 % between one of the isolates and the type strains of M. chelonae and M. abscessus confirmed that the isolates belong to a distinct species. The polyphasic characterization of these isolates, supported by DNA–DNA hybridization results,demonstrated that they share characteristics with M. chelonae–M. abscessus members, butconstitute a different species, for which the name Mycobacterium saopaulense sp. nov. is proposed. The type strain is EPM10906T (5CCUG 66554T5LMG 28586T5INCQS 0733T).

  1. Prevalencia y caracterización de la pérdida auditiva en trabajadores expuestos a ruido industrial de una planta eléctrica turbogenerada en un complejo petroquímico

    OpenAIRE

    Montiel-López, María; Corzo-Alvarez, Gilbert; Chacín-Almarza, Betulio; Rojas-González, Liliana; Quevedo, Ana; Lubo-Palma, Adonias; Rendiles, Hernando

    2006-01-01

    El propósito de este estudio fue determinar la prevalencia y caracterización de la pérdida auditiva por exposición laboral al ruido y su relación con otros factores, en trabajadores de una planta eléctrica turbogenerada en un complejo petroquímico del occidente de Venezuela. Se realizó un estudio de corte transversal donde se evaluaron 75 trabajadores a quienes se le practicó valoración médico ocupacional y audiometría tonal liminal. Se determinó el nivel de ruido en los lugares de trabajo me...

  2. Eficacia de la imaginería motora graduada en el síndrome de dolor regional complejo (SDRC) o Síndrome de Sudeck. Revisión Bibliográfica

    OpenAIRE

    Vidal Santos, Luis

    2016-01-01

    Introducción. El síndrome de dolor regional complejo (SDRC) constituye un trastorno neurológico en el que tras un evento nocivo previo, se produce una alteración del esquema corporal responsable de la aparición de un dolor ardiente, intenso e incapacitante. Su tratamiento constituye un gran reto para la ciencia, asentándose actualmente en la fisioterapia las mayores expectativas de futuro para la rehabilitación. Se ha decidido realizar este trabajo debido al poco conocimient...

  3. Buruli Ulcer (Mycobacterium ulcerans Infection)

    Science.gov (United States)

    ... detail/buruli-ulcer-(mycobacterium-ulcerans-infection)","@context":"http://schema.org","@type":"Article"}; العربية 中文 français русский español ... Buruli ulcer on a regular basis to share information, coordinate disease control and research efforts, and monitor ...

  4. El fenómeno de los desastres. Perspectiva transdisciplinar con el enfoque de los Sistemas Complejos The disaster phenomenon. A transdisciplinary perspective from the Complex Systems approach

    Directory of Open Access Journals (Sweden)

    Ania González Mora

    2008-04-01

    Full Text Available En el trabajo se realiza una breve reseña histórica del estudio de los desastres, como fenómenos objeto de estudio, resaltando lo sucedido en el continente latinoamericano. Se deja ver como la percepción del tema en nuestro continente está permeada por los problemas sociales que están vinculados con la ocurrencia de sucesos, mientras que el tratamiento en países industrializados se inclina hacia el estudio del suceso natural desde las ciencias naturales. En el trabajo se introducen conceptos utilizados en el lenguaje de los Sistemas Complejos, así como se plantea la necesidad de tratar transdisciplinariamente la investigación en el tema de desastres y de su introducción al currículo de las carreras biomédicas, utilizando como base epistemológica el pensamiento complejo.In this paper it’s fulfill a short historical review about the disaster studies theme, emphasizing what happens in Latin-American continent. We show how the theme perception in our countries is conditioned by the social problems, which are in narrow relation with occurring events, while the treatment about disasters in industrialized countries pass through the study of natural phenomenon from basic sciences. In this work we introduced some concepts used in the complex systems language and we talk about the necessity of the transdisciplinary treatment of disasters research and it introduction in curriculum of biomedicine career, using the epistemological foundations of complex thinking.

  5. Characterization of a Mycobacterium leprae antigen related to the secreted Mycobacterium tuberculosis protein MPT32

    NARCIS (Netherlands)

    Wieles, B.; van Agterveld, M.; Janson, A.; Clark-Curtiss, J.; Rinke de Wit, T.; Harboe, M.; Thole, J.

    1994-01-01

    Secreted proteins may serve as major targets in the immune response to mycobacteria. To identify potentially secreted Mycobacterium leprae antigens, antisera specific for culture filtrate proteins of Mycobacterium tuberculosis were used to screen a panel of recombinant antigens selected previously

  6. Coinfección por Mycobacterium malmoense y Mycobacterium tuberculosis en paciente con el síndrome de inmunodeficiencia humana

    Directory of Open Access Journals (Sweden)

    Lilian María Mederos Cuervo

    Full Text Available Se presenta un caso de coinfección por Mycobacterium malmoense y Mycobacterium tuberculosis en un paciente cubano con síndrome de inmunodeficiencia adquirida (sida, que producía enfermedad respiratoria y hepática respectivamente. Los cultivos realizados a partir de las muestras de esputo demostraron la presencia de una cepa micobacteriana no pigmentada de crecimiento lento perteneciente al grupo III de Runyon e identificada como Mycobacterium malmoense. A partir de los cultivos del tejido hepático extraído laparoscópicamente se aisló una cepa posteriormente identificada como Mycobacterium tuberculosis. El estudio anatomopatológico confirmó el diagnóstico de tuberculosis, el paciente recibió tratamiento específico y evolucionó clínicamente bien. Se reporta un caso infrecuente de coinfección por Mycobacterium, el cual describe el primer reporte de tuberculosis hepática en una paciente con sida en Cuba.

  7. Polymorphisms of twenty regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis

    Science.gov (United States)

    Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans or animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and the other members o...

  8. El complejo sinaptonémico en conejo cariotipo meiótico obtenido a partir de complejos sinaptonémicos en microesparcidos de espermatocitos paquiténicos

    Directory of Open Access Journals (Sweden)

    Juan José Filgueira D.

    1986-10-01

    Full Text Available Espermatocitos de conejo, fueron procesados mediante una técnica modificada de microesparcidos, que contribuyó a aumentar el conocimiento sobre el Complejo Sinaptonemico (CS y la sinapsis meiótica de los cromosomas del conejo. Los núcleos paquiténicos en microesparcidos, mostraron 22 Complejos inaptonémicos completamente formados que permitieron clasificarlos de acuerdo a la posición del centrómero; en los 22 grupos centroméricos, se determinaron las longitudes totales de los Complejos Sinaptonémicos, así como las correspondientes longitudes de los brazos de los bivalentes, para realizar un Idiograma. Los Complejos Sinaptonémicos (CS, aparecieron con restos de membrana nuclear en sus extremos y tres bivalentes presentaron residuos del nucleolo. El proceso de microesparcido fue seguido por microscopia óptica y se observe, como la técnica desarrollada para obtener los microesparcidos Hamada “de gota pendiente” aumentó la densidad de CSs por unidad de área. Las observaciones de los microesparcidos en grillas a traves del microscopio electrónico fueron corroboradas por observaciones de microesparcidos en placa, teñidos con plata, para microscopia de luz.

  9. Detección de Mycobacterium tuberculosis mediante la reacción en cadena de la polimerasa en una población seleccionada del noroccidente de México

    Directory of Open Access Journals (Sweden)

    Morán Moguel María Cristina

    2000-01-01

    Full Text Available Este estudio compara la detección de Mycobacterium tuberculosis mediante baciloscopia (tinción de Ziehl-Neelsen, cultivo en medio de Löwenstein-Jensen y reacción en cadena de la polimerasa (RCP realizada con ADN extraído directamente de distintos tipos de muestras. Se analizaron 252 muestras (114 de esputo, 96 de orina, 15 de LCR y 27 de otros tipos de 160 pacientes con sospecha de tuberculosis en cualquiera de sus formas que acudieron al Laboratorio de Patología Clínica del Hospital de Especialidades del Centro Médico Nacional de Occidente del Instituto Mexicano del Seguro Social. En todos los casos se realizó tinción de Ziehl-Neelsen, cultivo en medio de Löwenstein-Jensen y amplificación por RCP de un segmento de 285 pares de bases específico del complejo M. tuberculosis. De las 252 muestras, 18 fueron positivas para micobacterias no tuberculosas en el cultivo. De las 234 restantes, 12 (5,1% fueron positivas en la RCP y el cultivo, 174 (74,4% negativas en ambas pruebas, 47 (20,1% positivas en la RCP y negativas en el cultivo y 1 (0,4% negativa en la RCP y positiva en el cultivo; tomando el cultivo como prueba de referencia, la RCP proporcionó una sensibilidad de 92,3%, una especificidad de 78,7%, un valor predictivo positivo de 20,3% y un valor predictivo negativo de 99,4%. El límite de detección de la RCP en ADN extraído de cultivo fue de 10 fg (equivalente a 4 ó 5 micobacterias. También en comparación con el cultivo, la RCP identificó correctamente a la totalidad de las micobacterias del complejo M. tuberculosis. Tomando como prueba de referencia el cultivo, al analizar únicamente las muestras de esputo, la RCP directa proporcionó una sensibilidad de 90,9%, una especificidad de 89,5%, un valor predictivo positivo de 52,6% y un valor predictivo negativo de 98,7%. La RCP es una técnica sensible y específica para detectar el complejo M. tuberculosis en muestras tanto positivas como negativas en la baciloscopia. Un

  10. Mycobacterium avium Infection after Acupoint Embedding Therapy

    Directory of Open Access Journals (Sweden)

    Jiao Zhang, MD

    2017-09-01

    Full Text Available Summary:. Nontuberculous mycobacterium is a ubiquitous environmental organism that is unusual to cause a true infection, but it can cause severe cutaneous infections. In this case report, we present a successful treatment for a Chinese patient with Mycobacterium avium cutaneous infection after acupoint embedding therapy. We managed to conduct pathogenic detection, drug sensitive test, and multidisciplinary consultation. Finally, a systematic treatment strategy of nontuberculous mycobacterium was performed. Twenty-two-month follow-up revealed excellent outcome without any recurrence.

  11. Complejo tubo-ovárico: revisión de casos en el Hospital Nacional Cayetano Heredia (1990-1999

    Directory of Open Access Journals (Sweden)

    Ronald Roberto Sanchez Silva Torriani

    2003-07-01

    Full Text Available Objetivo: Determinar las características epidemiológicas, clínicas y de manejo del complejo tubo-ovárico (CTO.Material y métodos: Se realizó un estudio retrospectivo en 65 pacientes hospitalizadas con diagnóstico de complejo tubo-ovárico (CTO en el Servicio de Ginecología del Hospital Nacional Cayetano Heredia de Lima, Perú, entre Enero de 1990 y Diciembre de 1999. Se registraron datos demográficos, antecedentes patológicos, cuadro clínico, exámenes auxiliares, tratamiento médico, quirúrgico, hallazgos operatorios, complicaciones quirúrgicas, tiempo de hospitalización y seguimiento. Resultados: El complejo tubo-ovárico correspondió al 0.72% de las pacientes hospitalizadas en el servicio de Ginecología de este hospital. Este fue más frecuente entre la tercera y cuarta década de la vida (promedio = 31.9 años, en casadas (44.6% y multigestas (53.8%. El uso de dispositivos intrauterinos no tuvo relación estadísticamente significativa con la localización del CTO confirmado quirúrgicamente. Las manifestaciones clínicas más frecuentes fueron dolor pélvico (96.9%, fiebre (76.9%, dolor a la movilización cervical (60% y masa anexial (58.5%. La ecografía pélvica presentó mayor positividad en el diagnóstico de CTO (91.3%, luego de la laparoscopía (100%, aunque ésta se realizó solo en 4 casos. El drenaje de absceso más lavado de cavidad peritoneal tuvo similar porcentaje de complicaciones que las demás técnicas, pero en el seguimiento 2 pacientes (14.3% fueron reoperadas. En el seguimiento de 24 pacientes el dolor pélvico fue el problema más frecuente en 40% de casos. No hubo mortalidad en este estudio. Conclusiones: El complejo tubo-ovárico es una patología que compromete la capacidad reproductiva y función ovárica de la mujer. La ecografía es un examen que presentó alta positividad en el diagnóstico. La laparotomía exploratoria más drenaje de absceso y lavado de cavidad peritoneal es un tratamiento quir

  12. Relaciones espaciales y alimenticias del ensamblaje de reptiles del complejo cenagoso de Zapatosa, departamento del Cesar (Colombia

    Directory of Open Access Journals (Sweden)

    Guido Fabian Medina-Rangel

    2015-01-01

    Full Text Available Para conocer los factores ambientales que favorece la riqueza y abundancia de especies y grupos de reptiles, su preferencia por microhábitats, dieta de especies y diferencias entre hábitats en parámetros como tamaño de individuos, grado de detección y proporción de depredación, se realizaron salidas en áreas circundantes al complejo cenagoso de Zapatosa, entre noviembre de 2006 y octubre de 2007, abarcando la época de lluvias y la época seca en cinco tipos hábitats. Los hábitats con diferencias significativas entre variables ambientales y estructurales fueron: bosque de ribera, palmar, sabanas arboladas y bosque casmófito; el bosque de ribera y bosque seco no resultaron diferentes. La inclinación del terreno y el grado de intervención antrópica fueron las variables más relacionadas con la abundancia de las especies. Al aumentar la pendiente y la intervención antrópica disminuyó la riqueza y abundancia de reptiles en general y de serpientes y lagartos; con el aumento de la temperatura media ambiental la abundancia de reptiles disminuyó. Con el aumento de la cobertura herbácea y la disminución del diámetro de los árboles, disminuyó la riqueza de lagartos y serpientes. El tamaño corporal de los reptiles no fue diferente entre hábitats, aunque las tallas pequeñas predominaron en todos. Las serpientes presentaron menores valores de detección que los lagartos. El bosque de ribera presentó los valores más altos de detección. Un quinto de todas las lagartijas presentó algún signo de depredación y fue más alta en el palmar. Los reptiles terrestres fueron los más ricos y abundantes en el estudio. Los lagartos presentaron la mayor amplitud en el uso de hábitat y microhábitat, mientras que las serpientes Helicops danieli y Leptodeira septentrionalis y los lagartos Anolis gaigei, Gonatodes albogularis y Cnemidophorus lemniscatus en el uso de alimento. Los lagartos exhibieron mayor solapamiento en el uso de los recursos.

  13. Poynting's theorem for complex fields; El teorema de Poynting para campos complejos

    Energy Technology Data Exchange (ETDEWEB)

    Fernandez Guasti, M. [Universidad Autonoma Metropolitana-Unidad Iztapalapa, Mexico, D.F (Mexico)

    2001-04-01

    Poynting's theorem is derived for complex electromagnetic fields without invoking the harmonic dependence of the fields. This reformulation yields the functional form of a continuity equation. The definition of poynting's vector for complex fields reduces to its traditional definition for real fields without involving an extra factor of 1/2. [Spanish] Se presenta la derivacion del teorema de Poynting utilizando una representacion compleja de los campos electromagneticos. En esta reformulacion se obtiene cabalmente la forma funcional de una ecuacion de continuidad. Este resultado no requiere que los campos armonicos sean trenes de onda infinitos, de manera que con este formalismo es posible abordar el caso de pulsos electromagneticos. La definicion del vector Poynting para campos complejos se reduce a la definicion convencional si los campos son reales sin involucrar un factor de 1/2 adicional.

  14. Escolecita de aglomerados basálticos del complejo basal de Fuerteventura (Islas Canarias)

    OpenAIRE

    La Iglesia, Á.; Fernández Santín, Soledad; Hernández-Pacheco, A.

    1990-01-01

    Se caracteriza por análisis químico, microscopía óptica, difracción de Rayos X, análisis térmico diferencial, termogravimetría y espectroscopía de infrarrojos, una zeolita fibrosa perteneciente a la formación volcánica aglomerática del Complejo Basal de la isla de Fuerteventura. Los datos analíticos obtenidos confirman la identidad de esta zeolita fibrosa como escolecita. El análisis químico del mineral pone de manifiesto un elevado contenido en sodio, superior a los que aparecen en la biblio...

  15. Bioética global y pensamiento complejo. Hacia una emergente manera de ser

    Directory of Open Access Journals (Sweden)

    Sergio Néstor Osorio García

    2008-01-01

    Full Text Available La Bioética global como el pensamiento complejo sólo pueden entenderse en su novedad, si las comprendemos como estrategias emergentes que buscan enfrentar y responder a los desafíos de nuestra era planetaria. Desafíos que retan como nunca nuestra creatividad histórica para habitar la tierra. La consolidación y desarrollo de estas dos importantes estrategias del pensamiento y la acción, nos podrían ayudar en la construcción de un mundo más decente para los humanos y menos destructivo para con los ecosistemas no-humanos.

  16. Mecanismos de construcción-destrucción de patrones macroestructurales en microclimas como sistemas complejos

    Directory of Open Access Journals (Sweden)

    Sánchez-Muñoz, L.

    2006-10-01

    ón del núcleo 27Al (RMN, y mapas químicos de contenido en Na por microsonda electrónica (ME. Las pertitas pegmatíticas son sistemas complejos en cuyos feldespatos potásicos se han identificado dos periodos genéticos independientes de recristalización durante el enfriamiento geológico. En el primer periodo, en la estructura mineral se produce la construcción gradual de patrones macroscópicos regulares pseudoperiódicos, como consecuencia del efecto combinado de un ordenamiento local no-ergódico en la distribución Si/Al y de un efecto cooperativo global de la estructura del cristal. Desde las interfases incoherentes con el feldespato sódico surgen frentes de transformación a modo de avalanchas que pueden coalescer longitudinalmente y yuxtaponerse transversalmente para auto-ensamblarse, y finalmente evolucionar hasta formar dominios-macla (leyes Albita y Periclina, como unidad cristalina de construcción de la macroestructura. Estos procesos de auto-organización se activan cuando el sistema está abierto a la circulación de moléculas de agua catalizadoras para el ordenamiento de la red, al tiempo que existe una estimulación externa por tensiones tectónicas de cizalla. Durante el segundo periodo se destruyen los patrones estructurales creados previamente, por: (i maclados de relajación y deformación, (ii engrosamientos de dominios por disolución-reprecipitación debidos a interacciones con fluidos acuosos a baja temperatura, (iii reorientaciones de dominiosmacla según ley Periclina hacia orientaciones de ley Albita.

  17. Exploración sobre las decisiones estratégicas desde el pensamiento complejo.

    Directory of Open Access Journals (Sweden)

    Luz María Rivas Montoya

    2013-12-01

    Full Text Available La racionalidad, las decisiones estratégicas y el pensamiento complejo motivaron la construcción del problema de esta investigación, planteado en términos del carácter limitado y reduccionista de los criterios y referentes de decisión en la visión gerencial tradicional frente a una visión compleja de la realidad humana. La exploración de este problema se realizó desde y para el campo académico de la estrategia, con el propósito de mostrar algunas de las dificultades que se presentan en la definición e implementación de una estrategia corporativa en particular. El trabajo de campo se realizó en una empresa multinegocio del sector asegurador colombiano; los datos se obtuvieron a través de entrevistas semiestructuradas realizadas adiversos actores de la organización. El pensamiento complejo desde la perspectiva de Edgar Morin (1990 se constituyó en el marco de referencia teórica.   La hermenéutica de la acción de Ricœur (2010 iluminó la interpretación de los datos cualitativos; para su análisis se utilizó el sistema de codificación abierta, axial, selectiva y de procesoque proponen Strauss y Corbin (2002. En términos generales, tres fueron las categorías que emergieron de los datos: sinergia, como decisión estratégica relevante; des-humanización,como uno de los efectos de la decisión estratégica en los individuos, y rupturas de tejidos relacionales, como uno de los impactos que deja la sinergia en la organización bajo estudio.

  18. Molecular Characterization of the Resistance of Mycobacterium ...

    African Journals Online (AJOL)

    Purpose: To characterize the resistance of Mycobacterium tuberculosis to second line drugs using a line probe assay. Methods: Multi-drug resistant strains of Mycobacterium tuberculosis isolated between December 2008 and December 2009 were tested for resistance to fluoroquinolones and second-line injectable drugs ...

  19. Disseminated Mycobacterium avium infection in a cat

    OpenAIRE

    Barry, Maureen; Taylor, Judith; Woods, Paul

    2002-01-01

    A domestic shorthair cat was presented for lethargy and ataxia. Clinical findings included an abdominal mass, lumbosacral pain, ataxia. Aspirates from the liver and lymph nodes revealed intracellular, negative-staining rods. Treatment for presumptive mycobacterium infection was unsuccessful and the cat was euthanized. Disseminated Mycobacterium avium was confirmed on culture.

  20. Disseminated Mycobacterium avium infection in a cat.

    Science.gov (United States)

    Barry, Maureen; Taylor, Judith; Woods, J Paul

    2002-05-01

    A domestic shorthair cat was presented for lethargy and ataxia. Clinical findings included an abdominal mass, lumbosacral pain, ataxia. Aspirates from the liver and lymph nodes revealed intracellular, negative-staining rods. Treatment for presumptive mycobacterium infection was unsuccessful and the cat was euthanized. Disseminated Mycobacterium avium was confirmed on culture.

  1. Drug Resistance of Mycobacterium tuberculosis Complex among ...

    African Journals Online (AJOL)

    BACKGROUND: In Burkina Faso, there is no recent data about the level of drug resistance in Mycobacterium tuberculosis strains among newly diagnosed tuberculosis cases. OBJECTIVE: To provide an update of the primary drug resistance of mycobacterium tuberculosis among patients in Burkina faso. METHODS: ...

  2. MycoCAP - Mycobacterium Comparative Analysis Platform.

    Science.gov (United States)

    Choo, Siew Woh; Ang, Mia Yang; Dutta, Avirup; Tan, Shi Yang; Siow, Cheuk Chuen; Heydari, Hamed; Mutha, Naresh V R; Wee, Wei Yee; Wong, Guat Jah

    2015-12-15

    Mycobacterium spp. are renowned for being the causative agent of diseases like leprosy, Buruli ulcer and tuberculosis in human beings. With more and more mycobacterial genomes being sequenced, any knowledge generated from comparative genomic analysis would provide better insights into the biology, evolution, phylogeny and pathogenicity of this genus, thus helping in better management of diseases caused by Mycobacterium spp.With this motivation, we constructed MycoCAP, a new comparative analysis platform dedicated to the important genus Mycobacterium. This platform currently provides information of 2108 genome sequences of at least 55 Mycobacterium spp. A number of intuitive web-based tools have been integrated in MycoCAP particularly for comparative analysis including the PGC tool for comparison between two genomes, PathoProT for comparing the virulence genes among the Mycobacterium strains and the SuperClassification tool for the phylogenic classification of the Mycobacterium strains and a specialized classification system for strains of Mycobacterium abscessus. We hope the broad range of functions and easy-to-use tools provided in MycoCAP makes it an invaluable analysis platform to speed up the research discovery on mycobacteria for researchers. Database URL: http://mycobacterium.um.edu.my.

  3. INTERVENCIÓN AL COMPLEJO FERROCARRILERO EN AGUASCALIENTES, MÉXICO. DE BARRERA A ESPACIO PÚBLICO.

    Directory of Open Access Journals (Sweden)

    Ana Paulina Sotomayor

    2017-01-01

    Full Text Available La explosión de las ciudades hacia la periferia, ha provocado que los centros históricos queden en el olvido. Los centros históricos se caracterizan por albergar monumentos o sitios que esconden épocas importantes para las ciudades. Este texto, invita a conocer la historia ferroviaria de la ciudad de Aguascalientes; así como, la restauración que tuvieron los talleres de locomotoras tan importantes a partir del siglo XIX. El análisis partió diferenciando lo que es el patrimonio y lo que es la identidad, con la finalidad de caracterizar a los antiguos talleres en algún rubro. Posteriormente, se identificó lo realizado durante la intervención del espacio, siempre considerando que esta actividad fue el inicio de la vocación industrial, destacada hoy en día en la ciudad. El rescate y conservación realizados por Gobierno del Estado en años anteriores, convirtió parte de los talleres en un espacio donde convergen actividades recreativas, deportivas y sociales, a  través de un parque, un museo y un centro de convenciones, por mencionar algunos.

  4. Herencia de la resistencia al complejo Cercospora spp. en líneas de maíz tropical

    Directory of Open Access Journals (Sweden)

    Narro L. Luis

    2005-09-01

    Full Text Available Se evaluó un diseño dialélico (Griffing Modelo 1 entre 10 líneas endogámicas de maíz tropical contrastantes en su reacción a Cercospora spp. (5 tolerantes y 5 susceptibles, en tres localidades en lotes bajo siembra directa, con presencia natural del patógeno e inoculación artificial, para la variable índice de enfermedad (escala 1 a 5. En el análisis de varianza, por localidad y combinado, los efectos de habilidad combinatoria general (hcg, habilidad combinatoria específica (hce, y recíprocos resultaron altamente significativos; siendo >10 veces mayor los efectos de la hcg que los de hce, indicando predominio de la acción genética aditiva. Resultados también sugieren que la resistencia está controlada por efectos maternos o de citoplasma. La acumulación de genes de resistencia y la conformación de variedades sintéticas tolerantes es una alternativa a implementar en programas del mejoramiento de resistencia a Cercospora de maíces tropicales. ABSTRACT Inheritance of resistance to Cercospora Complex in tropical maize (zea mays inbred lines. Inheritance of resistance to the maize disease complex caused by Cercospora spp on tropical maize was studied by generating and evaluating a diallel mating model (Griffing model I using ten corn inbred lines with contrasting levels of disease reaction (5 tolerant and 5 susceptible. Parental lines and their crosses, both direct and reciprocal, were evaluated in three locations using conservation tillage and inoculated artificially with inoculum prepared using a mixture of foliar maize diseases collected from several farmer’s fields. Analysis of variance for the variable index of disease (scale 1 to 5, both locality and combined, the general combining ability (hcg, specific combining ability (hce, and reciprocal effects were highly significant, with the hcg portion being >10 times greater than the hce component, indicating that the genetic action is predominantly additive. Data collected suggested that maternal or cytoplasmic effects are important in controlling disease resistance. Accumulation of resistance genes through the development of synthetic varieties is an alternative to be implemented in maize breeding programs workin towards the development of gray leaf spot resistant tropical maize germplasm. Key words: Zea mays, Cercospora zeae maydis, gray leaf spot of maize, diallel mating, general combining ability, specific combining ability, reciprocal effects.

  5. Los peligros volcánicos asociados al complejo del Teide y los Rifts activos de Tenerife

    OpenAIRE

    Carracedo, Juan Carlos; Fra-Paleo, U.; Rodríguez Badiola, Eduardo; Pérez Torrado, Francisco José; Paris, Raphael; Guillou, Hervé; Scaillet, Stéphane; Hansen Machín, Álex

    2006-01-01

    Estos trabajos geológicos no habrían podido realizarse sin la oportuna y generosa financiación de Obra Social y Cultural de la Caja General de Ahorros de Canarias y el Laboratoire des Sciences du Climat et de l’Environnement (CEA-CNRS), Francia. Posteriormente, por la financiación dentro del marco del Plan Nacional de I+D, del proyecto de investigación CGL2005-00239.

  6. Breve historia del Complejo Industrial Sahagún, 1948-1988/95

    Directory of Open Access Journals (Sweden)

    ADRIÁN ESCAMILLA TREJO

    2013-10-01

    Full Text Available En este trabajo se analiza el funcionamiento económico de un importante complejo paraestatal, a través de tres de sus principales empresas: Diesel Nacional (DINA, Constructora Nacional de Carros de Ferrocarril (CONCARRIL y Siderúrgica Nacional (SIDENA, las cuales integraron durante algún tiempo uno de los ejes de la industria de bienes de capital y duraderos en México. El objetivo del ensayo es presentar los límites y alcances de esta experiencia industrial de Estado, proponiendo una forma de acercarse de manera más objetiva a la historia de este complejo industrial y sus empresas. La conclusión central es que las tres empresas cumplieron con el objetivo para el que fueron creadas: satisfacer la demanda creciente de los bienes que producían, con calidad y a bajo costo. Sin embargo, con altos niveles de endeudamiento por el subsidio otorgado a otras entidades estatales y a empresas privadas; y sin poder conformar un sólido núcleo tecnológico que les permitiera adaptar, innovar y reproducir endógenamente nuevos y mejores productos, por lo que las tres empresas continuaron dependiendo en mayor o menor medida de la asesoría tecnológica de firmas transnacionales líderes en su ramo. Abstract This paper analyzes the economic operation of an important “industrial state complex”, through its three main companies: National Diesel (DINA, National Construction Railcars (CONCARRIL and National Steel (SIDENA, which integrated for some time one of the axes of the capital goods and durable goods industry in Mexico. The article objective is to present the limits and scope of the State's industrial experience, proposing a way of getting more objectively at the history of this industrial complex and its companies. The central conclusion is that the three companies met the objective for which they were created: to satisfy the growing demand for the goods they produced, quality and cost. However, with high levels of indebtedness due the subsidy

  7. Beta-lactamases of Mycobacterium tuberculosis and Mycobacterium kansasii.

    Science.gov (United States)

    Segura, C; Salvadó, M

    1997-09-01

    Re-emergence of infectious diseases caused by mycobacteria as well as the emergence of multiresistant strains of Mycobacterium has promoted the research on the use of beta-lactames in the treatment of such diseases. Mycobacteria produce beta-lactamases: M. tuberculosis produces a wide-spectrum beta-lactamase whose behaviour mimicks those of Gram-negative bacteria. M. kansasii produces also beta-lactamase which can be inhibited by clavulanic acid. An overview on beta-lactamases from both species is reported.

  8. ¿COLOMBIA DEBE APOSTARLE AL SOFTWARE LIBRE, AL DE FUENTE ABIERTA O AL PROPIETARIO?

    Directory of Open Access Journals (Sweden)

    Luis Alberto Girón Murillo

    2014-06-01

    Full Text Available Es complejo para gestores y administradores conocer las implicaciones que la adopción de modelos de desarrollo de software tendría para una región. Este artículo ofrece una evaluación de las implicaciones dinámicas que para el desarrollo de software de una región tendría el apostar de forma excluyente al software libre, al software de fuente abierta y al software propietario. Se desarrolló un modelo de simulación en Vensim de la dinámica de sistemas en donde se evaluaron 3 escenarios: uno en donde se adopta de forma excluyente el software libre, uno en donde se adopta el software propietario y uno en donde se adopta el software de fuente abierta. Se ofrecen descripciones y explicaciones de los resultados que sugieren las potencialidades de cada uno de los modelos de desarrollo evaluados.

  9. EL TURISMO SUSTENTABLE EN EL ESTADO DE TAMAULIPAS, MÉXICO COMPLEJO TURISTICO SUSTENTABLE

    Directory of Open Access Journals (Sweden)

    Ma. de Lourdes Arias Gómez

    2013-06-01

    Full Text Available El turismo sustentable es de gran importancia, tanto para la economía del país como para la persona que gusta del eco turismo. Asimismo, el turismo sustentable considera el cuidado del medio ambiente y la mejora de la población. El sur del estado de Tamaulipas cuenta con un sinnúmero de recursos naturales, tiene mar, playa, ríos, lagunas, manantiales, cenotes, montañas, flora, fauna, por ende, el turista podrá practicar surf, jetski, velero, rapel, ciclismo de montaña, buceo, espeleología, caminata, caza, pesca, observar aves, fotografía, entre muchas otras actividades. Por ese motivo, presentamos la propuesta de un complejo turístico sustentable “El sur mágico de Tamaulipas”, el cual cubre el circuito turístico del sur del estado, se propone la construcción de ocho cedes, distribuidos en los municipios del sur del estado. Los visitantes podrán hospedarse en dos o más cedes todo por un mismo precio. Por ser Tamaulipas parte de la huasteca, proponemos a los turistas la oportunidad de conocer las cuatro huastecas tamaulipeca, veracruzana, potosina e hidalguense. Consideramos que Tampico debe ser el centro del circuito, porque todos los lugares que seleccionamos están a no más de tres horas de distancia por carretera.

  10. Complejo de piscinas calentadas por energía solar Saizburgo – Austria

    Directory of Open Access Journals (Sweden)

    Garstenauer, G.

    1980-06-01

    Full Text Available The main feature of this complex, located in Dorfgastein (Salzburg is to use the «greenhouse » effect to heat the water instead of the solar panels that have been used up until now in similar constructions. The chief installations of this work are: — two saunas, one for ladies, the other for gentlemen; — a solarium with swimming lanes; and — two hot water round swimming pools. Centers such as this permit the bathing season to be prolonged, which otherwise is normally limited to the summertime months, to all the year round.

    La característica principal de este complejo, situado en Dorfgastein (Saizburgo, es la utilización del efecto de «invernadero » para calentar el agua en lugar de los paneles solares empleados hasta ahora en construcciones similares. Las principales instalaciones de esta obra son: — dos saunas, una para señoras y otra para caballeros; — un solario con canales de natación; y — dos piscinas circulares de agua caliente. Centros de este tipo permiten prolongar la temporada de baños, normalmente reducida a los meses de verano, a prácticamente todo el año.

  11. Methanol production by Mycobacterium smegmatis

    International Nuclear Information System (INIS)

    Weisman, L.S.; Ballou, C.E.

    1988-01-01

    Mycobacterium smegmatis cells produce [ 3 H]methanol when incubated with [methyl- 3 H]methionine. The methanol is derived from S-adenosylmethionine rather than methyltetrahydrofolate. M. smegmatis cells carboxymethylate several proteins, and some of the methanol probably results from their demethylation, but most of the methanol may come from an unidentified component with a high gel mobility. Although methanol in the medium reached 19 μM, it was not incorporated into the methylated mannose polysaccharide, a lipid carrier in this organism

  12. Complejos de halógeno acetatos de uranio (IV y torio (IV con sulfoxidos y fosfinoxidos

    Directory of Open Access Journals (Sweden)

    Omar Velasquez

    2010-09-01

    Full Text Available En este trabajo se ha Investigado el efecto sterico sobre los modos de coordinación del grupo carboxilato (unidentado, bidentado y puente, en complejos de halógeno acetato con fosfinóxidos y sulfóxidos y la correlación de los resultadoscon el modelo "Cone Angle" e\\ cual ha sido descrito en otra publicación previa' Los complejos preparados son: M(RCO ^ .nL donde n = 4, M = Th ó U, L = Me^SO (dmso, R = CF,3 y L = Me3P0 (tmpo; n = 3, M = Th, L = tmpo, R = CF3 y L = ppo o dmso, R OCCI3; M = U, L = t p p o , R =CF3 y L =dmso, dpso, R = C C l 3 ; n = 2 , M = T h , L = tppo, R = CF3, CHCI^On^ 1, M = Th ó U, L =dmso.

  13. Sales de imidazolio funcionalizadas: síntesis y formación de complejos metálicos

    OpenAIRE

    Sol Sánchez, Daniel

    2014-01-01

    El presente trabajo describe la síntesis de una sal de imidazolio funcionalizada con un grupo difenilfosfino en el carbono de la posición 4 del anillo heterocíclico y el estudio de su reactividad orientada a la formación de complejos metálicos, singularmente aquellos que contienen el carbeno N-heterocíclico derivado de la desprotonación del catión imidazolio.

  14. La mutabilidad de los lazos de parentesco. Revisión del Complejo de Edipo y el tabú del incesto

    Directory of Open Access Journals (Sweden)

    Santiago Peidro

    2014-05-01

    Full Text Available El objetivo de este escrito es revisar los modos de parentesco tradicionales que se desprenden del complejo de Edipo y del tabú del incesto sostenidos desde el psicoanálisis freudiano como condiciones normativas de la cultura. La hipótesis aquí sostenida es que las relaciones de parentesco son variables y se actualizan conforme a los cambios sociales. De este modo, la intención de esta revisión, es entrecruzar las ideas de Judith Butler con el psicoanálisis lacaniano a fin de preguntarnos si los modos de parentesco ratificados desde el psicoanálisis son a su vez los que presuponen al sujeto en la heterosexualidad normativa, y si acaso este procedimiento es la condición para una vida simbólica o culturalmente inteligible. // The aim of this revision is to look through the traditional ways of kinship that come off from the Oedipus complex and the incest's taboo, sustained as normative conditions of Culture from the Freudian psychoanalysis point of view. The hypothesis that I propose is that kinships are variable and are based on social practices. Therefore, the intention here is to interwine Judith Butler's and Lacanian psychoanalysis´ approaches to wonder if the ways of kinship ratified by the psychoanalysis are also the ones that presuppose a subject in a normative heterosexuality and if this procedure is the condition for a symbolic or culturally intelligible life.

  15. Infección pulmonar por Mycobacterium avium en paciente VIH/SIDA: Primer reporte en Perú

    Directory of Open Access Journals (Sweden)

    Juan Carrasco

    Full Text Available El complejo Mycobacterium avium (MAC es un patógeno que se encuentra en el medioambiente y causa infecciones tanto en pacientes inmunocompetentes como inmunocomprometidos. Se presenta el caso de un paciente VIH positivo varón de 38 años infectado por P. jirovecii y aparentemente infectado por Mycobacterium tuberculosis desde el año 2009, el cual fue tratado con antibioticoterapia para pneumocistosis y terapia antituberculosis (TB logrando mejoría parcial. En el año 2012 se le realizó nuevamente examen de cultivo y un nuevo tratamiento anti TB, frente a la sospecha de estar en presencia de una cepa de TB multidrogorresistente se recomienda realizar la identificación micobacteriana. El examen de cultivo fue positivo y el resultado genotípico resultó positivo para MAC. Se reporta el primer caso de un paciente VIH/SIDA con infección pulmonar por MAC en el Perú, así como una breve revisión de los aspectos epidemiológicos, clínicos y de tratamiento

  16. Mycobacterium arupense, Mycobacterium heraklionense, and a Newly Proposed Species, "Mycobacterium virginiense" sp. nov., but Not Mycobacterium nonchromogenicum, as Species of the Mycobacterium terrae Complex Causing Tenosynovitis and Osteomyelitis.

    Science.gov (United States)

    Vasireddy, Ravikiran; Vasireddy, Sruthi; Brown-Elliott, Barbara A; Wengenack, Nancy L; Eke, Uzoamaka A; Benwill, Jeana L; Turenne, Christine; Wallace, Richard J

    2016-05-01

    Mycobacterium terrae complex has been recognized as a cause of tenosynovitis, with M. terrae and Mycobacterium nonchromogenicum reported as the primary etiologic pathogens. The molecular taxonomy of the M. terrae complex causing tenosynovitis has not been established despite approximately 50 previously reported cases. We evaluated 26 isolates of the M. terrae complex associated with tenosynovitis or osteomyelitis recovered between 1984 and 2014 from 13 states, including 5 isolates reported in 1991 as M. nonchromogenicum by nonmolecular methods. The isolates belonged to three validated species, one new proposed species, and two novel related strains. The majority of isolates (20/26, or 77%) belonged to two recently described species: Mycobacterium arupense (10 isolates, or 38%) and Mycobacterium heraklionense (10 isolates, or 38%). Three isolates (12%) had 100% sequence identity to each other by 16S rRNA and 99.3 to 100% identity by rpoB gene region V sequencing and represent a previously undescribed species within the M. terrae complex. There were no isolates of M. terrae or M. nonchromogenicum, including among the five isolates reported in 1991. The 26 isolates were susceptible to clarithromycin (100%), rifabutin (100%), ethambutol (92%), and sulfamethoxazole or trimethoprim-sulfamethoxazole (70%). The current study suggests that M. arupense, M. heraklionense, and a newly proposed species ("M. virginiense" sp. nov.; proposed type strain MO-233 [DSM 100883, CIP 110918]) within the M. terrae complex are the major causes of tenosynovitis and osteomyelitis in the United States, with little change over 20 years. Species identification within this complex requires sequencing methods. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  17. Edipo sin complejos: la ley en crisis bajo los efectos del capitalismo

    Directory of Open Access Journals (Sweden)

    Arribas, Sonia

    2007-02-01

    Full Text Available Lacan’s reading of the Freudian Oedipal myth amounts to its historization and demythologization: it is an illustration of the discourse of the master –an effect of language and the fundamental principle of subjectivation. This article argues that the condition of possibility of this historization stems from the historical crisis of the Oedipus complex as the ideological discourse that was characteristic of the liberal stage of capitalism. In Seminar XVII Lacan establishes a dialectics between the discourse of the master and the logic of capitalism that can be translated as a conceptualization of that crisis, and also, and in a parallel way, as the most accurate articulation of the emergence of psychoanalysis as a historical practice.La lectura lacaniana del Edipo freudiano equivale a la historización y desmitologización del mito con el objetivo de convertirlo en la ejemplificación del discurso del amo en tanto que efecto del lenguaje y principio fundamental de la subjetivación. Este artículo argumenta que la condición de posibilidad de esta historización proviene de la crisis histórica del complejo del Edipo en cuanto que el discurso ideológico característico de la etapa liberal del capitalismo. La dialéctica que establece Lacan en el Seminario XVII entre el discurso del amo y la lógica del capitalismo puede traducirse como conceptualización de esa crisis y también, y de manera paralela, como la más fina articulación de la emergencia del psicoanálisis como práctica histórica.

  18. Whole genome sequence analysis of Mycobacterium suricattae

    KAUST Repository

    Dippenaar, Anzaan; Parsons, Sven David Charles; Sampson, Samantha Leigh; Van Der Merwe, Ruben Gerhard; Drewe, Julian Ashley; Abdallah, Abdallah; Siame, Kabengele Keith; Gey Van Pittius, Nicolaas Claudius; Van Helden, Paul David; Pain, Arnab; Warren, Robin Mark

    2015-01-01

    Tuberculosis occurs in various mammalian hosts and is caused by a range of different lineages of the Mycobacterium tuberculosis complex (MTBC). A recently described member, Mycobacterium suricattae, causes tuberculosis in meerkats (Suricata suricatta) in Southern Africa and preliminary genetic analysis showed this organism to be closely related to an MTBC pathogen of rock hyraxes (Procavia capensis), the dassie bacillus. Here we make use of whole genome sequencing to describe the evolution of the genome of M. suricattae, including known and novel regions of difference, SNPs and IS6110 insertion sites. We used genome-wide phylogenetic analysis to show that M. suricattae clusters with the chimpanzee bacillus, previously isolated from a chimpanzee (Pan troglodytes) in West Africa. We propose an evolutionary scenario for the Mycobacterium africanum lineage 6 complex, showing the evolutionary relationship of M. africanum and chimpanzee bacillus, and the closely related members M. suricattae, dassie bacillus and Mycobacterium mungi.

  19. Whole genome sequence analysis of Mycobacterium suricattae

    KAUST Repository

    Dippenaar, Anzaan

    2015-10-21

    Tuberculosis occurs in various mammalian hosts and is caused by a range of different lineages of the Mycobacterium tuberculosis complex (MTBC). A recently described member, Mycobacterium suricattae, causes tuberculosis in meerkats (Suricata suricatta) in Southern Africa and preliminary genetic analysis showed this organism to be closely related to an MTBC pathogen of rock hyraxes (Procavia capensis), the dassie bacillus. Here we make use of whole genome sequencing to describe the evolution of the genome of M. suricattae, including known and novel regions of difference, SNPs and IS6110 insertion sites. We used genome-wide phylogenetic analysis to show that M. suricattae clusters with the chimpanzee bacillus, previously isolated from a chimpanzee (Pan troglodytes) in West Africa. We propose an evolutionary scenario for the Mycobacterium africanum lineage 6 complex, showing the evolutionary relationship of M. africanum and chimpanzee bacillus, and the closely related members M. suricattae, dassie bacillus and Mycobacterium mungi.

  20. Mycobacterium intracellulare Infection Mimicking Progression of Scleroderma

    DEFF Research Database (Denmark)

    Krabbe, Simon; Engelhart, Merete; Thybo, Sören

    2017-01-01

    This case report describes a patient with scleroderma who developed Mycobacterium intracellulare infection, which for more than a year mimicked worsening of her connective tissue disorder. The patient was diagnosed with scleroderma based on puffy fingers that developed into sclerodactyly, abnormal......, unfortunately with significant scarring. Immunodeficiency testing was unremarkable. In summary, an infection with Mycobacterium intracellulare was mistaken for an unusually severe progression of scleroderma....

  1. Multiplexed Quantitation of Intraphagocyte Mycobacterium tuberculosis Secreted Protein Effectors

    Directory of Open Access Journals (Sweden)

    Fadel Sayes

    2018-04-01

    Full Text Available Summary: The pathogenic potential of Mycobacterium tuberculosis largely depends on ESX secretion systems exporting members of the multigenic Esx, Esp, and PE/PPE protein families. To study the secretion and regulation patterns of these proteins while circumventing immune cross-reactions due to their extensive sequence homologies, we developed an approach that relies on the recognition of their MHC class II epitopes by highly discriminative T cell receptors (TCRs of a panel of T cell hybridomas. The latter were engineered so that each expresses a unique fluorescent reporter linked to specific antigen recognition. The resulting polychromatic and multiplexed imaging assay enabled us to measure the secretion of mycobacterial effectors inside infected host cells. We applied this novel technology to a large panel of mutants, clinical isolates, and host-cell types to explore the host-mycobacteria interplay and its impact on the intracellular bacterial secretome, which also revealed the unexpected capacity of phagocytes from lung granuloma to present mycobacterial antigens via MHC class II. : Sayes et al. develop an approach to express distinct fluorescent reporters that is based on the recognition of specific Mycobacterium tuberculosis MHC class II epitopes by highly discriminative T cell hybridomas. This multiplexed technology allows the study of secretion, subcellular location, and regulation patterns of these instrumental protein members. Keywords: mycobacterium tuberculosis, type VII secretion systems, intracellular bacteria, T-cell hybridomas, mycobacterial virulence factors, bacterial antigen presentation, lentiviral vectors, reporter T cells, in vivo antigen presentation, protein localization

  2. Whole-genome sequence analysis of the Mycobacterium avium complex and proposal of the transfer of Mycobacterium yongonense to Mycobacterium intracellulare subsp. yongonense subsp. nov.

    Science.gov (United States)

    Castejon, Maria; Menéndez, Maria Carmen; Comas, Iñaki; Vicente, Ana; Garcia, Maria J

    2018-06-01

    Bacterial whole-genome sequences contain informative features of their evolutionary pathways. Comparison of whole-genome sequences have become the method of choice for classification of prokaryotes, thus allowing the identification of bacteria from an evolutionary perspective, and providing data to resolve some current controversies. Currently, controversy exists about the assignment of members of the Mycobacterium avium complex, as is for the cases of Mycobacterium yongonense and 'Mycobacterium indicus pranii'. These two mycobacteria, closely related to Mycobacterium intracellulare on the basis of standard phenotypic and single gene-sequences comparisons, were not considered a member of such species on the basis on some particular differences displayed by a single strain. Whole-genome sequence comparison procedures, namely the average nucleotide identity and the genome distance, showed that those two mycobacteria should be considered members of the species M. intracellulare. The results were confirmed with other whole-genome comparison supplementary methods. According to the data provided, Mycobacterium yongonense and 'Mycobacterium indicus pranii' should be considered and renamed and included as members of M. intracellulare. This study highlights the problems caused when a novel species is accepted on the basis of a single strain, as was the case for M. yongonense. Based mainly on whole-genome sequence analysis, we conclude that M. yongonense should be reclassified as a subspecies of Mycobacterium intracellulareas Mycobacterium intracellularesubsp. yongonense and 'Mycobacterium indicus pranii' classified in the same subspecies as the type strain of Mycobacterium intracellulare and classified as Mycobacterium intracellularesubsp. intracellulare.

  3. Complete Genome Sequence of Mycobacterium phlei Type Strain RIVM601174

    KAUST Repository

    Abdallah, A. M.; Rashid, M.; Adroub, S. A.; Arnoux, M.; Ali, Shahjahan; van Soolingen, D.; Bitter, W.; Pain, Arnab

    2012-01-01

    Mycobacterium phlei is a rapidly growing nontuberculous Mycobacterium species that is typically nonpathogenic, with few reported cases of human disease. Here we report the whole genome sequence of M. phlei type strain RIVM601174.

  4. Complete Genome Sequence of Mycobacterium phlei Type Strain RIVM601174

    KAUST Repository

    Abdallah, A. M.

    2012-05-24

    Mycobacterium phlei is a rapidly growing nontuberculous Mycobacterium species that is typically nonpathogenic, with few reported cases of human disease. Here we report the whole genome sequence of M. phlei type strain RIVM601174.

  5. Mycobacterium chelonae infections associated with bee venom acupuncture.

    Science.gov (United States)

    Cho, Sun Young; Peck, Kyong Ran; Kim, Jungok; Ha, Young Eun; Kang, Cheol-In; Chung, Doo Ryeon; Lee, Nam Yong; Song, Jae-Hoon

    2014-03-01

    We report 3 cases of Mycobacterium chelonae infections after bee venom acupuncture. All were treated with antibiotics and surgery. Mycobacterium chelonae infections should be included in the differential diagnosis of chronic skin and soft tissue infections following bee venom acupuncture.

  6. Mycobacterium alsense sp. nov., a scotochromogenic slow grower isolated from clinical respiratory specimens.

    Science.gov (United States)

    Tortoli, Enrico; Richter, Elvira; Borroni, Emanuele; Cabibbe, Andrea M; Capitolo, Eleonora; Cittaro, Davide; Engel, Regina; Hendricks, Oliver; Hillemann, Doris; Kristiansen, Jette E; Mariottini, Alessandro; Schubert, Sabine; Cirillo, Daniela M

    2016-01-01

    The name 'Mycobacterium alsiense', although reported in 2007, has not been validly published. Polyphasic characterization of three available strains of this species led us to the conclusion that they represent a distinct species within the genus Mycobacterium. The proposed novel species grows slowly and presents pale yellow-pigmented colonies. Differentiation from other mycobacteria is not feasible on the basis of biochemical and cultural features alone while genetic analysis, extended to eight housekeeping genes and one spacer region, reveals its clear distinction from all other mycobacteria. Mycobacterium asiaticum is the most closely related species on the basis of 16S rRNA gene sequences (similarity 99.3 %); the average nucleotide identity between the genomes of the two species is 80.72 %, clearly below the suggested cut-off (95-96 %). The name Mycobacterium alsense sp. nov. is proposed here for the novel species and replaces the name 'M. alsiense', ex Richter et al. 2007, given at the time of isolation of the first strain. The type strain is TB 1906T ( = DSM 45230T = CCUG 56586T).

  7. The draft genome of Mycobacterium aurum , a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae

    Directory of Open Access Journals (Sweden)

    Jody Phelan

    2015-01-01

    Full Text Available Mycobacterium aurum (M. aurum is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02 Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related to 96.2% for rrs (streptomycin, capreomycin. We observed two homologous genes encoding the catalase-peroxidase enzyme (katG that is associated with resistance to isoniazid. Similarly, two emb B homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum , this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae.

  8. The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae

    KAUST Repository

    Phelan, Jody

    2015-06-04

    Mycobacterium aurum (M. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02 Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related) to 96.2% for rrs (streptomycin, capreomycin). We observed two homologous genes encoding the catalase-peroxidase enzyme (katG) that is associated with resistance to isoniazid. Similarly, two embB homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum, this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae.

  9. The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae

    KAUST Repository

    Phelan, Jody; Maitra, Arundhati; McNerney, Ruth; Nair, Mridul; Gupta, Antima; Coll, Francesc; Pain, Arnab; Bhakta, Sanjib; Clark, Taane G.

    2015-01-01

    Mycobacterium aurum (M. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02 Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related) to 96.2% for rrs (streptomycin, capreomycin). We observed two homologous genes encoding the catalase-peroxidase enzyme (katG) that is associated with resistance to isoniazid. Similarly, two embB homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum, this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae.

  10. The draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae.

    Science.gov (United States)

    Phelan, Jody; Maitra, Arundhati; McNerney, Ruth; Nair, Mridul; Gupta, Antima; Coll, Francesc; Pain, Arnab; Bhakta, Sanjib; Clark, Taane G

    2015-09-01

    Mycobacterium aurum (M. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related) to 96.2% for rrs (streptomycin, capreomycin). We observed two homologous genes encoding the catalase-peroxidase enzyme (katG) that is associated with resistance to isoniazid. Similarly, two embB homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum, this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae. Copyright © 2015 Asian-African Society for Mycobacteriology. Published by Elsevier Ltd. All rights reserved.

  11. Intervención de conservación de emergencia en el complejo arqueológico Aypate

    OpenAIRE

    Huisa Palomino, Lorenzo; Ministerio de Cultura

    2014-01-01

    En el marco del Proyecto de Investigación Arqueológica Aypate, con fines de diagnóstico para su puesta en uso social, se están desarrollando labores como la elaboración del diagnóstico del estado de conservación de los sectores y edificaciones, la identificación y mapeo de zonas de riesgo, factores y agentes de deterioro y el nivel de vulnerabilidad que presenta cada uno de los componentes arquitectónicos del Complejo Arqueológico Aypate; así como el monitoreo constante del nivel de debilidad...

  12. Antecedentes históricos sobre el Complejo Forestal y Maderero Panguipulli, provincia de Valdivia, Centro-sur de Chile

    OpenAIRE

    Barrena Ruiz, José; Hernando Arrese, Maite; Rojas Marchini, Fernanda

    2016-01-01

    El Complejo Forestal y Maderero Panguipulli, empresa surgida en 1971 en la provincia de Valdivia, administró un área superior a 400.000 ha y empleó a más de 3.000 trabajadores bajo un esquema de comanejo entre obreros y Estado. El objetivo fue indagar en los procesos sociales que influyeron en la conformación de esta empresa. Se desarrolló una revisión bibliográfica que incluyó artículos científicos, trabajos de tesis, informes y libros. Adicionalmente, se realizaron dos entrevistas en profun...

  13. Prevalencia de traumatismos dentoalveolares en pacientes infantiles del complejo asistencial Dr. Sótero del Río

    OpenAIRE

    Castro Brezzo, P.F.; Dreyer Arroyo, E.

    2012-01-01

    En el presente estudio descriptivo de incidencia, 604 niños menores de 15 años fueron examinados y atendidos en la Clínica de Odontopediatría del Complejo Asistencial Dr. Sotero del Río, con diagnóstico de Traumatismo Dentoalveolar (TDA), desde Enero a Diciembre del 2010. El propósito de este estudio fue caracterizar los TDA de acuerdo a la clasificación de Andreasen y Andreasen, determinar su frecuencia y distribución estacional, tipo y número de dientes afectados, distribución de género y e...

  14. La educación de la mirada: Percepción, pensamiento complejo y competencias visuales

    OpenAIRE

    Jiménez-Montano, M. (Mayra); Flores-Ramos, J.E. (José E.)

    2016-01-01

    Los autores proponen un modelo pedagógico para los estudiantes de diseño arquitectónico de primer año que parte de la interacción de la triada de la problematización de la percepción visual, el pensamiento complejo de Edgar Morin y el desarrollo de las competencias visuales. A partir de estos preceptos se diseñan actividades pedagógicas que estimulan experiencias significativas en el alumnado para el desarrollo de una mirada educada, profunda y analítica. La herramienta utiliza...

  15. La enseñanza y el aprendizaje de los números complejos: un estudio en el nivel universitario

    OpenAIRE

    Pardo, Tomás; Gómez, Bernardo

    2007-01-01

    Presentamos algunos de los resultados más relevantes de un estudio sobre la problemática de la enseñanza y el aprendizaje de los números complejos. El estudio se ha dirigido a recabar información para sustentar sugerencias de intervención en las pautas educativas en relación con esta temática. We present some of the most relevant results of a study concerning the teaching and learning of complex numbers. The study is focused on collecting data to s...

  16. Amarre, rendimiento y calidad del fruto en naranja con aplicación de un complejo hormonal

    OpenAIRE

    Galván-Luna, Juan José; Briones-Encinia, Florencio; Rivera-Ortiz, Patricio; Valdes-Aguilar, Luis Alonso; Soto-Hernández, Marcos; Rodríguez-Alcázar, Jorge; Salazar-Salazar, Ovidio

    2009-01-01

    En cítricos el uso de reguladores de crecimiento incrementa el amarre de frutos y en consecuencia la productividad. El objetivo del presente trabajo fue determinar el efecto de un complejo hormonal de origen natural (auxinas, giberelinas y citocininas) en el amarre, rendimiento y calidad del fruto en naranja (Citrus sinensis (L.) Osb) del grupo navel cvs. "Washington" y "Thomson", en árboles de 15 años de edad. El estudio se realizó en el municipio de Ciudad Victoria, Tamaulipas, México duran...

  17. Enfermedad por Mycobacterium simiae y "Mycobacterium sherrisii" en la Argentina

    Directory of Open Access Journals (Sweden)

    Lucía Barrera

    2010-08-01

    Full Text Available Se presenta información reunida retrospectivamente sobre casos de micobacteriosis originados por Mycobacterium simiae (n = 4 y "M. sherrisii" (n = 6. Los casos ocurrieron entre pacientes con sida (n = 6, historia de silicosis (n = 2 o tuberculosis previa (n = 1. Un caso se perdió luego de diagnosticado y nueve fueron tratados con esquemas terapéuticos basados en claritromicina, etambutol y quinolonas. La respuesta fue muy pobre: cinco pacientes fallecieron (cuatro eran HIV positivos, tres permanecieron crónicos y sólo uno curó. Estas micobacterias originaron 2.1% de los casos de micobacteriosis registrados en un período de ocho años. La distinción de estas micobacterias raras de otras más frecuentes por métodos moleculares rápidos, parece ser clínicamente útil para advertir sobre la dificultad que puede presentar el tratamiento. Sin embargo, la diferenciación genotípica entre M. simiae y "M. sherrisii" parecería no ser clínicamente relevante, dado que no quedaron expuestas características que distingan a los pacientes afectados por los dos microorganismos tan estrechamente relacionados.

  18. Construcción de bases conceptuales de la gestión ambiental en clave del pensamiento ambiental estético complejo. Estudio de caso de las organizaciones ambientales de la Universidad Nacional Sede Manizales = Conceptual construction of environmental management in terms of complex environmental aesthetic thought. Case study of environmental organizations at the National University at Manizales

    OpenAIRE

    Villota Martínez, Doris Adriana

    2012-01-01

    La investigación se forja desde nuevas perspectivas para abordar el tema de la gestión ambiental, una que tiene como base las urdimbres del orden que se muestran a través del discurso decolonial de Arturo Escobar, sobre el desarrollo aplicado al discurso de las cumbres ambientales internacionales y de las políticas nacionales. El otro pilar se configura con las tramas de la vida evidenciadas por medio del pensamiento estético complejo, que retoma las nociones de las teorías de la compleji...

  19. Complejo residencial económico y popular - P.E.E.P. - Nueva Liguria – Italia

    Directory of Open Access Journals (Sweden)

    Ceroni, Eugenio

    1979-03-01

    Full Text Available This article describes the construction of a building compound, built with government funds. This group contains, besides the housing units, both in 21 m-high tower form and in 18 m-high elongated building, a series of public and private services, namely: — Parking lots. — Underground garages. — Landscaped areas. — Play and sports grounds. — Religious, social, medical, cultural, and shopping centres, etc.

    Se describe en este artículo la realización de un complejo de edificios, construidos con ayuda estatal. Este complejo, además de los edificios destinados a viviendas —cuya tipología varía desde edificios-torre, con una altura de 21 m, hasta edificios lineales con una altura de 18 m—, está dotado de una serie de servicios públicos y privados tales como: — Aparcamientos. — Garajes subterráneos. — Zonas verdes. — Campos de juego. — Centros religioso, social, médico, cultural, comercial, etc.

  20. Gestión organizacional en entornos complejos por parte de las Mipymes del sector servicios de la ciudad de Sincelejo

    Directory of Open Access Journals (Sweden)

    Jorge Luis Del Rio Vásquez

    2017-11-01

    Full Text Available Con el propósito de determinar los modelos de gestión organizacional en entornos complejos que practican las MIPYMES del sector servicios en la ciudad de Sincelejo - Sucre - Colombia, se adelanta la presente investigación. Para ello es importante tener en cuenta su gestión actual, toda vez que estas representan en su mayoría el tejido empresarial de las economías locales y nacionales. El presente artículo pretende un análisis, a la luz de la teoría y antecedentes empíricos, que demuestre la relación entre la gestión organizacional y los índices de perdurabilidad o tasas de mortalidad de las MIPYMES del sector servicios, definiendo los principales enfoques de modelos de gestión organizacional en cabeza de autores como Koontz y Weihrich, (1998, De la Rosa, (2002, Wong, (2000, entre otros. Finalmente, las MIPYMES del sector, desde su enfoque funcional u operativo e informalidad no se aprestan a un enfoque basado en procesos, por lo tanto, los esfuerzos en materia de fortalecimiento y consolidación empresarial, estarían encaminados al desarrollo de la gestión del talento humano, la gestión comercial, la gestión gerencial, la gestión de producción, la gestión financiera, la gestión tecnológica y la gestión de calidad.

  1. Variación espacio-temporal de plantas vasculares acuáticas en el complejo cenagoso del bajo Sinú, Córdoba, Colombia

    Directory of Open Access Journals (Sweden)

    Nabi Del Socorro Pérez-Vásquez

    2015-09-01

    Full Text Available Se determinó la variación espacio temporal de las plantas vasculares acuáticas en las ciénagas de María Arriba, San Sebastián, Purísima y Momil del complejo cenagoso del bajo Sinú (CCBS, en el departamento de Córdoba (Colombia, abarcando los periodos hidrológicos ocurridos durante el año 2011. El área de estudio se dividió en cuatro sectores y mediante el método de intercepción en línea se determinó la abundancia y frecuencia de las hidrófitas. En cada sector se extendió una línea de 200 m perpendicular al litoral y sobre ella se ubicaron cuadrantes de 1 m2 cada 50 m, en los que, se determinó la cobertura y frecuencia de las diferentes especies  Se registraron 39659 individuos distribuidos en 24 familias, 30 géneros y 35 especies. La mayor abundancia de especies se presentó en Momil con 18582 individuos, siendo la familia Lemnaceae la más representativa. El análisis de varianza (ANOVA indica que no existen diferencias estadísticas significativas en la abundancia de la especies de plantas acuáticas de los  meses de muestreo (F=1,04; GL=11; p = 0,083, sin embargo, se evidenció la incidencia del período hidrológico sobre la dinámica de crecimiento de las plantas, algunas especies desaparecen en época seca, otras sobreviven un periodo corto de tiempo, mientras que otras como Lemna minor L y Salvinia auriculata Aubl aumentan en época de lluvia.

  2. Riqueza del complejo chisa (Coleoptera: Melolonthidae en cuatro agroecosistemas del Cauca, Colombia

    Directory of Open Access Journals (Sweden)

    Morón R. Miguel A.

    2005-12-01

    Full Text Available La composición y variación de la riqueza del complejo de larvas Melolonthidae se examinó en cuatro circunstancias agroecológicas en los municipios de Caldono y Buenos Aires, Cauca (1400 a 1500 m.s.n.m, 21,5ºC. y 2000 mm/año de precipitación promedio. Quincenalmente, durante un año, se visitaron parcelas de media hectárea de yuca, pastizal, cafetal y bosque, en cada parcela y ocasión se realizaron 10 muestreos ( 1m2 por 15 cm de profundidad. Se colectaron 10.261 larvas pertenecientes a 32 especies y 12 géneros de Melolonthidae, así: Phyllophaga, Plectris, Astaena, Macrodactylus, Ceraspis, Barybas, Isonychus, Anomala Callistethus, Strigoderma, Leucothyreus, Cyclocephala. La riqueza de especies presentó variaciones estadísticamente significativas entre localidades (F=87.24 p =2.72-18, muestreos (F=22.29 p =5.18-13, parcelas (F= 23.39 p=1.40-13 así como entre la mayoría de interacciones. Los promedios de riqueza fueron: yuca (4.5 , pastizal (3.6, café (2.1 y bosque (2.2, cada uno con grandes fluctuaciones que hicieron la diferencia. Se discuten las curvas de isodensidades de cada parcela y sus implicaciones en el programa de manejo. Abstract Richness of the white-grub complex (Coleoptera: Melolonthidae in agroecosystems of the Cauca province, Colombia. The composition and variation in the richness of the complex of rhizophagous Melolonthidae larvae was examinated in four agroecological zones in the Municipality of Caldono (1400-1500 m, 21.5ºC, average rainfall 2000 mm/yr with complementary surveys in the neighbouring site of Buenos Aires, Cauca. Half-hectare plots (cassava, pastures, coffee and wooded areas were selected and visited every 15 days (total of 15 visits per plot, and a total of 10 samples were taken (1 m2 by 15 cm deep. In total, 10,261 larvae of 32 species were collected: Phyllophaga, Plectris, Astaena, Macrodactylus, Ceraspis, Barybas, Isonychus, Anomala Callistethus, Strigoderma, Leucothyreus, Cyclocephala

  3. Mycobacterium mageritense Parotitis in an Immunocompetent Adult.

    Science.gov (United States)

    Okabe, Taro; Sasahara, Teppei; Suzuki, Jun; Onishi, Tsubasa; Komura, Masayoshi; Hagiwara, Shigehiro; Suzuki, Hiromichi; Morisawa, Yuji

    2018-03-01

    Mycobacterium mageritense , a rapidly growing mycobacterium, is a rare clinical pathogen. Furthermore, parotitis due to non-tuberculosis mycobacterium is very rare in adults. Herein, we report the first case of M. mageritense parotitis in an immunocompetent adult. A 40-year-old man presented with swelling in a left parotid lesion. He was diagnosed with parotitis. The culture from the parotid abscess grew M. mageritense . He was unsuccessfully treated with levofloxacin monotherapy. Trimethoprim-sulfamethoxazole was added, leading to some clinical response; however, the erythema persisted despite 14 months of antibiotic therapy. Subsequently, the skin lesion was surgically removed. The antibiotic treatment was ceased a week after surgery as the postoperative course was uneventful and the lesion had improved. No recurrence was noted at 7 months after surgery. Although extremely rare, M. mageritense can cause parotitis in immunocompetent adults, and may not be sufficiently treated with antibiotics alone.

  4. Draft Genome Sequence of Mycobacterium chimaera Type ...

    Science.gov (United States)

    We report the draft genome sequence of the type strain Mycobacterium chimaera Fl-0169T, a member of the Mycobacterium avium complex (MAC). M. chimaera Fl-0169T was isolated from a patient in Italy and is highly similar to strains of M. chimaera isolated in Ireland, though Fl-0169T possesses unique virulence genes. Evidence suggests that M. avium, M. intracellulare, and M. chimaera are differently virulent and a comparative genomic analysis is critically needed to identify diagnostic targets that reliably differentiate species of MAC. With treatment costs for Mycobacterium infections estimated to be >$1.8 B annually in the U.S., correct species identification will result in improved treatment selection, lower costs, and improved patient outcomes.

  5. Mycobacterium franklinii sp. nov., a species closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group.

    Science.gov (United States)

    Lourenço Nogueira, Christiane; Simmon, Keith E; Chimara, Erica; Cnockaert, Margo; Carlos Palomino, Juan; Martin, Anandi; Vandamme, Peter; Brown-Elliott, Barbara A; Wallace, Richard; Cardoso Leão, Sylvia

    2015-07-01

    Two isolates from water, D16Q19 and D16R27, were shown to be highly similar in their 16S rRNA, 16S-23S internal transcribed spacer (ITS), hsp65 and rpoB gene sequences to 'Mycobacterium franklinii' DSM 45524, described in 2011 but with the name not validly published. They are all nonpigmented rapid growers and are related phenotypically and genetically to the Mycobacterium chelonae-Mycobacterium abscessus group. Extensive characterization by phenotypic analysis, biochemical tests, drug susceptibility testing, PCR restriction enzyme analysis of the hsp65 gene and ITS, DNA sequencing of housekeeping genes and DNA-DNA hybridization demonstrated that 'M. franklinii' DSM 45524, D16Q19 and D16R27 belong to a single species that is separated from other members of the M. chelonae-M. abscessus group. On the basis of these results we propose the formal recognition of Mycobacterium franklinii sp. nov. Strain DSM 45524(T) ( = ATCC BAA-2149(T)) is the type strain.

  6. Permian depositional age of metaturbidites of the Duque de York Complex, southern Chile: U-Pb SHRIMP data and palynology Edad pérmica de sedimentación de las metaturbiditas del Complejo Duque de York, sur de Chile: datos mediante U-Pb SHRIMP y palinología

    Directory of Open Access Journals (Sweden)

    Fernando A Sepúlveda

    2010-07-01

    Full Text Available The Duque de York Complex (DYC is part of the low grade metamorphic accretionary complexes of the pre-Andean Patagonian 'basement'. It is a sedimentary succession exposed along the western margin of southernmost South America. New U-Pb zircon ages and palynological data restrict the maximum depositional age of the DYC to the limit between the early Permian (Kungurian and the middle Permian (Roadian. The palynological association recorded in the DYC, characterized mainly by Gymnospermopsida pollen, indicates a humid environment of forest with an under-growth of ferns. Regional paleogeographic correlations point out that an interpretation of DYC as an autochthonous terrane cannot be discarded, contrasting with previous hypotheses which suggest an allochthonous character for this complex.El Complejo Duque de York (CDY forma parte de los complejos metamórficos acrecionarios del 'basamento' pre-Andino de la Patagonia, correspondiendo a una sucesión sedimentaria que añora a lo largo del margen occidental austral de Sudamérica. Nuevas edades U-Pb en circón, en combinación con información palinológica, permiten acotar la maxima edad de depósito posible del CDY al límite entre el Pérmico temprano (Kunguriano y el Pérmico medio (Roadiano. La asociación palinológica registrada en el CDY está caracterizada por Gymnospermopsida, e indica un ambiente húmedo de bosque con sotobosque de heléchos. Las correlaciones paleogeográñcas apuntan a que la condición de terreno autóctono del CDY no puede ser descartada, lo que se contrapone a hipótesis anteriores, las que sugieren un carácter alóctono para este complejo.

  7. Photodynamic inactivation of the models Mycobacterium phlei and Mycobacterium smegmatis in vitro

    Science.gov (United States)

    Bruce-Micah, R.; Gamm, U.; Hüttenberger, D.; Cullum, J.; Foth, H.-J.

    2009-07-01

    Photodynamic inactivation (PDI) of bacterial strains presents an attractive potential alternative to antibiotic therapies. Success is dependent on the effective accumulation in bacterial cells of photochemical substances called photosensitizers, which are usually porphyrins or their derivatives. The kinetics of porphyrin synthesis after treatment with the precursor ALA and the accumulation of the Chlorin e6 and the following illumination were studied. The goal was to estimate effectivity of the destructive power of these PS in vitro in respect of the physiological states of Mycobacteria. So the present results examine the cell destruction by PDI using ALA-induced Porphyrins and Chlorin e6 accumulated in Mycobacterium phlei and Mycobacterium smegmatis, which serve as models for the important pathogens Mycobacterium tuberculosis, Mycobacterium leprae and Mycobacterium bovis. We could show that both Mycobacterium after ALA and Chlorin e6 application were killed by illumination with light of about 662 nm. A reduction of about 97% could be reached by using a lightdose of 70 mW/cm2.

  8. Targeting phenotypically tolerant Mycobacterium tuberculosis

    Science.gov (United States)

    Gold, Ben; Nathan, Carl

    2016-01-01

    While the immune system is credited with averting tuberculosis in billions of individuals exposed to Mycobacterium tuberculosis, the immune system is also culpable for tempering the ability of antibiotics to deliver swift and durable cure of disease. In individuals afflicted with tuberculosis, host immunity produces diverse microenvironmental niches that support suboptimal growth, or complete growth arrest, of M. tuberculosis. The physiological state of nonreplication in bacteria is associated with phenotypic drug tolerance. Many of these host microenvironments, when modeled in vitro by carbon starvation, complete nutrient starvation, stationary phase, acidic pH, reactive nitrogen intermediates, hypoxia, biofilms, and withholding streptomycin from the streptomycin-addicted strain SS18b, render M. tuberculosis profoundly tolerant to many of the antibiotics that are given to tuberculosis patients in a clinical setting. Targeting nonreplicating persisters is anticipated to reduce the duration of antibiotic treatment and rate of post-treatment relapse. Some promising drugs to treat tuberculosis, such as rifampicin and bedaquiline, only kill nonreplicating M. tuberculosis in vitro at concentrations far greater than their minimal inhibitory concentrations against replicating bacilli. There is an urgent demand to identify which of the currently used antibiotics, and which of the molecules in academic and corporate screening collections, have potent bactericidal action on nonreplicating M. tuberculosis. With this goal, we review methods of high throughput screening to target nonreplicating M. tuberculosis and methods to progress candidate molecules. A classification based on structures and putative targets of molecules that have been reported to kill nonreplicating M. tuberculosis revealed a rich diversity in pharmacophores. However, few of these compounds were tested under conditions that would exclude the impact of adsorbed compound acting during the recovery phase of

  9. Estimación de profundidades del Complejo Plutónico Navarrete mediante procesamiento gravimétrico

    Directory of Open Access Journals (Sweden)

    Flavia A Croce

    2009-06-01

    Full Text Available Se presenta el estudio gravimétrico en la región noreste del macizo de Somuncurá provincia de Río Negro con el objetivo de modelar la geometría y estimar las máximas profundidades del Complejo Plutónico Navarrete, emplazado en corteza superior. A la carta de anomalías de Bouguer se la filtró con el objeto de separar dichas anomalías en anomalía regional (que responde a características corticales profundas y anomalía residual (la cual contiene efectos de masas locales y cercanas a la superficie. Para ello se optó por el método de prolongación ascendente de campos potenciales, ya que en un estudio cualitativo fue el más apropiado para la resolución de dicho problema en particular. El afloramiento oeste del Complejo Plutónico Navarrete muestra un valor positivo de anomalía de gravedad de +5 mGal. Por el contrario el afloramiento este de dicho granito, presenta valores negativos entre -5 y -10 mGal. A partir del espectro de frecuencia radial aplicado a la carta de anomalías residuales de Bouguer, se estimaron las máximas profundidades de los afloramientos este y oeste del complejo intrusivo Navarrete, con valores de 8 km y de 6,5 km respectivamente.A gravimetric study is presented in the northeast region of the Somuncurá massif, with the objective of analyzing the geometry and maximum depths of the Navarrete Plutonic Complex, located in the upper crust. A Bouguer anomaly map was filtered in order to separating these anomalies in regional anomaly (which should contain the effects of deep crustal features and residual anomaly (which should contain the effects of local and near surface masses. The process used was the upward continuation method of the potential field. As a result, the west Navarrete granite formation shows a positive value of +5 mGal for gravity anomalies. On the contrary, the east Navarrete granite formation presents negative values between -5 and -10 mGal. A two dimensional power spectrum was applied to the

  10. Detection of Mycobacterium Tuberculosis by using PCR

    International Nuclear Information System (INIS)

    Suhadi, F; Dadang-Sudrajat; Maria-Lina, R.

    1996-01-01

    Polymerase Chain Reaction (PCR) procedure using three primary set derived from repetitive DNA sequence specific to mycobacteria was used to diagnose pathogenic Mycobacterium tuberculosis. The assay was specific for M. tuberculosis and could be used to detect the amount DNA less than 10 -9 g

  11. Mycobacterium bovis Infection of Red Fox, France.

    Science.gov (United States)

    Michelet, Lorraine; De Cruz, Krystel; Hénault, Sylvie; Tambosco, Jennifer; Richomme, Céline; Réveillaud, Édouard; Gares, Hélène; Moyen, Jean-Louis; Boschiroli, María Laura

    2018-06-01

    Mycobacterium bovis infection in wild red foxes was found in southern France, where livestock and other wildlife species are infected. Foxes frequently interact with cattle but have been underestimated as a reservoir of M. bovis. Our results suggest a possible role of the red fox in the epidemiology of bovine tuberculosis.

  12. Mycobacterium tuberculosis monoarthritis in a child

    Directory of Open Access Journals (Sweden)

    Rosenberg Alan M

    2008-09-01

    Full Text Available Abstract A child with isolated Mycobacterium tuberculosis monoarthritis, with features initially suggesting oligoarthritis subtype of juvenile idiopathic arthritis, is presented. This patient illustrates the need to consider the possibility of tuberculosis as the cause of oligoarthritis in high-risk pediatric populations even in the absence of a tuberculosis contact history and without evidence of overt pulmonary disease.

  13. Investigating Mycobacterium chelonae-abscessus Complex

    Centers for Disease Control (CDC) Podcasts

    2011-11-17

    Keith Simmon, scientist at Isentio US discusses research that was done while he was at ARUP laboratories, discusses a new classification of Mycobacterium chelonae-abscessus complex.  Created: 11/17/2011 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 11/22/2011.

  14. Safety assessment in primary Mycobacterium tuberculosis smear ...

    African Journals Online (AJOL)

    Introduction Tuberculosis (TB) is caused by Mycobacterium tuberculosis and is transmitted mainly through aerosolization of infected sputum which puts laboratory workers at risk in spite of the laboratory workersf risk of infection being at 3 to 9 times higher than the general public. Laboratory safety should therefore be ...

  15. Molecular Characterization of the Resistance of Mycobacterium ...

    African Journals Online (AJOL)

    Abstract. Purpose: To characterize the resistance of Mycobacterium tuberculosis to second line drugs using a line probe assay. Methods: ... Marne-la-Coquette,. France). Bacterial isolates contained in 500 µl of liquid culture were heat- inactivated at 95 °C for 30 min and then sonicated for 12 min. Finally, the suspension was ...

  16. Peritoneal tuberculosis due to Mycobacterium caprae

    Directory of Open Access Journals (Sweden)

    T. Nebreda

    2016-01-01

    Full Text Available The incidence of tuberculosis in humans due to Mycobacterium caprae is very low and is almost confined to Europe. We report a case of a previously healthy 41-year-old Moroccan with a 6 month history of abdominal pain, weight loss, fatigue and diarrhea. A diagnosis of peritoneal tuberculosis due to M. caprae was made.

  17. The epidemiology of Mycobacterium leprae: recent insight

    NARCIS (Netherlands)

    van Beers, S. M.; de Wit, M. Y.; Klatser, P. R.

    1996-01-01

    Leprosy is still a health problem in many countries. Because the causative organism, Mycobacterium leprae cannot be cultured in vitro, it is virtually impossible to assess exposure, and the onset of infection and disease. As a consequence, the chain of infection, considered as the relationships

  18. Modelling the Transitional Dynamics of Mycobacterium Tuberculosis ...

    African Journals Online (AJOL)

    The World Health Organization's targets of eliminating Tuberculosis (TB) by 2050 is challenged by the emergence and spread of drug resistance TB. However, the traditional mechanism of resistance is that of acquired resistance, whereby the mycobacterium Tuberculosis (MTB) strain develops mutations under selective ...

  19. Mitogen-activated protein kinases mediate Mycobacterium

    Indian Academy of Sciences (India)

    CD44, an adhesion molecule, has been reported to be a binding site for Mycobacterium tuberculosis (M. tuberculosis) in macrophages and it also mediates mycobacterial phagocytosis, macrophage recruitment and protective immunity against pulmonary tuberculosis in vivo. However, the signalling pathways that are ...

  20. Otomastoiditis Caused by Mycobacterium abscessus, the Netherlands

    NARCIS (Netherlands)

    van Ingen, Jakko; Looijmans, Frank; Mirck, Piet; Dekhuijzen, Richard; Boeree, Martin; van Soolingen, Dick

    2010-01-01

    To the Editor: Nontuberculous mycobacteria (NTM) are increasingly recognized as human pathogens (1). Otomastoiditis is a rare extrapulmonary NTM disease type first described in 1976; Mycobacterium chelonae-M. abscessus group bacteria, which are rapidly growing NTM, are the most frequent causative

  1. ELECTROPHORETIC MOBILITY OF MYCOBACTERIUM AVIUM COMPLEX ORGANISMS

    Science.gov (United States)

    The electrophoretic mobilities (EPMs) of thirty Mycobacterium avium Complex (MAC) organisms were measured. The EPMs of fifteen clinical isolates ranged from -1.9 to -5.0 µm cm V-1s-1, and the EPMs of fifteen environmental isolates ranged from -1...

  2. Chronic leg ulcer caused by Mycobacterium immunogenum

    NARCIS (Netherlands)

    Loots, Miriam A. M.; de Jong, Menno D.; van Soolingen, Dick; Wetsteyn, José C. F. M.; Faber, William R.

    2005-01-01

    Rare tropical skin diseases are seen more frequently in Western countries because of the increased popularity of visiting tropical regions. A 55-year-old white man developed a painless leg ulcer after traveling in Guatemala and Belize. A mycobacterium was cultured from a biopsy specimen and was

  3. Identification of Immunotopes against Mycobacterium leprae as ...

    African Journals Online (AJOL)

    Purpose: To determine the surface epitopes of Mycobacterium leprae (M. leprae) and evaluate their efficacy in the production of anti-M. leprae antibodies in an animal model. Methods: Blood samples were obtained from 34 patients suffering from lepromatous leprosy. Antibodies were obtained from the samples, ...

  4. Seroprevalence of Mycobacterium avium SSP paratuberculosis ...

    African Journals Online (AJOL)

    This study aimed to determine the seroprevalence of antibodies for Mycobacterium avium subspecies paratuberculosis (MAP) in dairy cattle in the Jimma zone of Ethiopia in 2011. A random sample of 29 herds was selected, and all mature cattle within these herds had a blood sample taken. Serum was tested in duplicate, ...

  5. Granulomatous lobular mastitis secondary to Mycobacterium fortuitum.

    Science.gov (United States)

    Kamyab, Armin

    2016-12-16

    Granulomatous lobular mastitis is a rare inflammatory disease of the breast of unknown etiology. Most present as breast masses in women of child-bearing age. A 29-year-old female presented with a swollen, firm and tender right breast, initially misdiagnosed as mastitis. Core needle biopsy revealed findings consistent with granulomatous lobular mastitis, and cultures were all negative for an infectious etiology. She was started on steroid therapy to which she initially responded well. A few weeks later she deteriorated and was found to have multiple breast abscesses. She underwent operative drainage and cultures grew Mycobacterium fortuitum . Granulomatous lobular mastitis is a rare inflammatory disease of the breast. The definitive diagnose entails a biopsy. Other causes of chronic or granulomatous mastitis should be ruled out, including atypical or rare bacteria such as Mycobacterium fortuitum . This is the first reported case of granulomatous mastitis secondary to Mycobacterium fortuitum . With pathologic confirmation of granulomatous mastitis, an infectious etiology must be ruled out. Atypical bacteria such as Mycobacterium fortuitum may not readily grow on cultures, as with our case. Medical management is appropriate, with surgical excision reserved for refractory cases or for drainage of abscesses.

  6. El Complejo de Cambio en Suelos del Municipio de Marinilla [Antioquia] según el Método de Brown

    Directory of Open Access Journals (Sweden)

    Galiano S. Francisco

    1959-12-01

    Full Text Available Se estudió el complejo de cambio en suelos aluviales recientes, terrazas medias y terrazas altas del Municipio de Marinilla por el método rápido de Brown. El grado de saturación, bajo en todos ellos, se acentúa en los suelos de las terrazas más antiguas y nos indica el bajo nivel de fertilidad de estos suelos. La extracción del hidrógeno de cambio con acetato cálcico coincide con la que efectúa el acetato amónico, excepto para los suelos más ricos en materia orgánica con los que proporciona resultados más altos la solución extractora de acetato cálcico.

  7. La Política Social en el Estado de Bienestar: el desafío de los sistemas complejos

    Directory of Open Access Journals (Sweden)

    PIERPAOLO DONATI

    1987-01-01

    Full Text Available Es incorrecto asumir que la actual crisis en los medios del estado de bienestar supone también una crisis estructural en la política social; la política social en la sociedad postindustrial debe ser distinguida, estructural y culturalmente, del estado de bienestar. Así, se discuten la naturaleza de la ciudadanía y de los valores en la sociedad post-industrial. Los sistemas sociales post-industriales se caracterizan por la complejidad y la diferenciación social. Las tendencias actuales van hacia la desregulación, la descentralización, y la privatización. Se hace necesario entender un nuevo marco conceptual para estos procesos sociales complejos; el articulo hace una tentativa para definir ese marco basándose en el interaccionismo simbólico Talcott Parsons y Niklas Luhmann.

  8. Diseño de complejos binucleares de cobre en estudios biomiméticos de la tirosinasa.

    Directory of Open Access Journals (Sweden)

    Rolando Calero

    2015-10-01

    Full Text Available Se ha estudiado la reactividad de ciertos metaloenzimas de cobre y algunos complejos de Cobre sintéticos que presentan reactividad frente a sustratos, a través de la interacción con el oxígeno molecular, además se ha observado en las varias formas en como ocurre esta interacción y la razón por la cual  porque unos actúan con el oxigeno para ser transportadores como las Hemocianinas y otros formando aductos para mediar reacciones químicas hacia ciertos sustratos fenólicos. También se propone una revisión de los mecanismos para la hidroxilación de fenoles y la oxidación a quinonas, encontrando una dependencia atribuible a factores estéricos y redox en la unión catalizador-sustrato.

  9. Enfermedad por Mycobacterium simiae y "Mycobacterium sherrisii" en la Argentina Disease due to Mycobacterium simiae and "Mycobacterium sherrisii" in Argentina

    Directory of Open Access Journals (Sweden)

    Lucía Barrera

    2010-08-01

    Full Text Available Se presenta información reunida retrospectivamente sobre casos de micobacteriosis originados por Mycobacterium simiae (n = 4 y "M. sherrisii" (n = 6. Los casos ocurrieron entre pacientes con sida (n = 6, historia de silicosis (n = 2 o tuberculosis previa (n = 1. Un caso se perdió luego de diagnosticado y nueve fueron tratados con esquemas terapéuticos basados en claritromicina, etambutol y quinolonas. La respuesta fue muy pobre: cinco pacientes fallecieron (cuatro eran HIV positivos, tres permanecieron crónicos y sólo uno curó. Estas micobacterias originaron 2.1% de los casos de micobacteriosis registrados en un período de ocho años. La distinción de estas micobacterias raras de otras más frecuentes por métodos moleculares rápidos, parece ser clínicamente útil para advertir sobre la dificultad que puede presentar el tratamiento. Sin embargo, la diferenciación genotípica entre M. simiae y "M. sherrisii" parecería no ser clínicamente relevante, dado que no quedaron expuestas características que distingan a los pacientes afectados por los dos microorganismos tan estrechamente relacionados.A revision of mycobacterial disease due to M simiae (n = 4 and "M. sherrisii" (n = 6 identified during an eight-year period is presented. Cases occurred among patients with AIDS (n = 6, previous history of silicosis (n = 2 or tuberculosis (n = 2. One case was lost to follow-up and the remaining nine responded poorly to chemotherapy based on clarithromycin, ethambutol and fluoroquinolones. Five patients died of whom four were HIV-positive, three remained chronic and one was cured. These microorganisms originated 2.1% of mycobacterioses cases detected in an eight-year period. Timely identification of this group of uncommon mycobacteria by molecular methods seems to be clinically relevant in order to warn of difficulties inherent to the treatment. However, the distinction between both closely related microorganisms might not be crucial for case

  10. Cryopreservation of Mycobacterium bovis isolates

    Directory of Open Access Journals (Sweden)

    Cássia Yumi Ikuta

    2016-11-01

    Full Text Available Research, development of new biotechnological methods, diagnostic tests, confirmation of results, and reinvestigations are possible because of the availability of well-preserved living organisms maintained without any changes. Cryopreservation is a simpler, more reliable and long-term stable method for culture maintenance. Storage temperature and composition of the suspending vehicle are factors that affect the viability of mycobacterial strains. Three vehicles and three storage temperatures were evaluated to define a suitable cryoprotective medium for the preservation of Mycobacterium bovis strains. Colonies of sixteen M. bovis isolates were used to prepare the suspensions, which were then added to three vehicles: sterile 0.85% saline solution (SS, Middlebrook 7H9 broth (7H9, and Middlebrook 7H9 broth with sodium pyruvate (7H9p replacing glycerol. Aliquots of these suspensions were frozen by three different methods, directly in the -20°C freezer, directly in the -80°C freezer, and at -196°C by immersion in liquid nitrogen (LN. The frozen aliquots were thawed at room temperature after 45, 90 and 120 days. Mycobacterial viability was assessed by counting the living cells on plates of Stonebrink medium before and after the freezing procedure. Storage at -20°C exhibited a lower recovery of M. bovis compared to storage at -80°C (Dunn’s test, p=0.0018 and LN (Dunn’s test, p=0.0352. There was no statistically significant difference between storage at -80°C and in LN (Dunn’s test, p=0.1403, yet -80°C showed better results than LN. All three suspending vehicles showed no statistically significant difference in terms of viability (Friedman’s test, p=0.7765. Given the low loss proportion of 5% during storage at -20°C and the high cost equipment required for storage at -80°C and LN, we recommend storage at -20°C or -80°C, when this is available, for preservation of M. bovis field strains.

  11. Mycobacterium talmoniae sp. nov., a slowly growing mycobacterium isolated from human respiratory samples.

    Science.gov (United States)

    Davidson, Rebecca M; DeGroote, Mary Ann; Marola, Jamie L; Buss, Sarah; Jones, Victoria; McNeil, Michael R; Freifeld, Alison G; Elaine Epperson, L; Hasan, Nabeeh A; Jackson, Mary; Iwen, Peter C; Salfinger, Max; Strong, Michael

    2017-08-01

    A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable. The bacteria were acid-fast stain-positive, rod-shaped and exhibited growth after 7-10 days on solid media at temperatures ranging from 25 to 42°C. Colonies were non-pigmented, rough and slightly raised. Analyses of matrix-assisted laser desorption ionization time-of-flight profiles showed no matches against a reference library of 130 mycobacterial species. Full-length 16S rRNA gene sequences were identical for the two isolates, the average nucleotide identity (ANI) between their genomes was 99.7 % and phylogenetic comparisons classified the novel mycobacteria as the basal most species in the slowly growing Mycobacterium clade. Mycobacterium avium is the most closely related species based on rpoB gene sequence similarity (92 %), but the ANI between the genomes was 81.5 %, below the suggested cut-off for differentiating two species (95 %). Mycolic acid profiles were more similar to M. avium than to Mycobacterium simiae or Mycobacterium abscessus. The phenotypic and genomic data support the conclusion that the two related isolates represent a novel Mycobacterium species for which the name Mycobacterium talmoniae sp. nov. is proposed. The type strain is NE-TNMC-100812T (=ATCC BAA-2683T=DSM 46873T).

  12. ANÁLISIS DESCRIPTIVO DE LA RESPUESTA DEL COMPLEJO PULPO-DENTINAL DE ÓRGANOS DENTALES DE UNA POBLACIÓN PREHISPÁNICA CAZADORA-RECOLECTORA

    Directory of Open Access Journals (Sweden)

    María Clemencia Vargas Vargas

    2011-12-01

    Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;} Antecedentes: El diente es de gran importancia cuando se trata de caracterizar individuos, poblaciones vivas o desaparecidas. Este componente es el que más resiste a la descomposición, debido a que al terminar su calcificación, el diente se conforma definitivamente y por tal razón no sufre ningún cambio. A excepción del desgaste mecánico o atrición y la acumulación de dentina secundaria. Objetivo: Deducir los posibles factores etiológicos que ocasionaron grandes desgastes dentales y condujeron a una rápida respuesta del complejo pulpo dentinal, en los individuos pertenecientes a una población prehispánica de cazadores-recolectores, del precerámico temprano y tardío cuya dieta era basada en alimentos fibrogénicos y abrasivos. Materiales y Métodos: Estudio descriptivo transversal donde se tomaron 15 piezas dentales, pertenecientes a dos poblaciones cazadoras-recolectoras: Precerámico temprano (Chequa y Precerámico tardío (Aguazuque.  A estos dos grupos se tomaron las muestras para realizar un análisis clínico, radiográfico y microscópico. Resultados: Disminución evidente en el tamaño de la corona clínica dental, se observaron diferentes grados de desgaste dental con exposición dentinaria delimitado por esmalte en su circunferencia. La dentina expuesta por el desgaste dental presenta

  13. ANÁLISIS DESCRIPTIVO DE LA RESPUESTA DEL COMPLEJO PULPO-DENTINAL DE ÓRGANOS DENTALES DE UNA POBLACIÓN PREHISPÁNICA CAZADORA-RECOLECTORA

    Directory of Open Access Journals (Sweden)

    María Clemencia Vargas Vargas

    2012-01-01

    Full Text Available Antecedentes: El diente es de gran importancia cuando se trata de caracterizar individuos, poblaciones vivas o desaparecidas. Este componente es el que más resiste a la descomposición, debido a que al terminar su calcificación, el diente se conforma definitivamente y por tal razón no sufre ningún cambio. A excepción del desgaste mecánico o atrición y la acumulación de dentina secundaria. Objetivo: Deducir los posibles factores etiológicos que ocasionaron grandes desgastes dentales y condujeron a una rápida respuesta del complejo pulpo dentinal, en los individuos pertenecientes a una población prehispánica de cazadores-recolectores, del precerámico temprano y tardío cuya dieta era basada en alimentos fibrogénicos y abrasivos. Materiales y Métodos: Estudio descriptivo transversal donde se tomaron 15 piezas dentales, pertenecientes a dos poblaciones cazadoras-recolectoras: Precerámico temprano (Chequa y Precerámico tardío (Aguazuque.  A estos dos grupos se tomaron las muestras para realizar un análisis clínico, radiográfico y microscópico. Resultados: Disminución evidente en el tamaño de la corona clínica dental, se observaron diferentes grados de desgaste dental con exposición dentinaria delimitado por esmalte en su circunferencia. La dentina expuesta por el desgaste dental presenta un color amarillo, las superficies oclusales e incisales se presentan lisas. Ninguna de las piezas dentarias presentó signos clínicos de caries. Respecto al examen radiográfico, los datos obtenidos confirmaron los hallazgos clínicos. Conclusiones: El desgaste dental es la característica clínica que más se presentó, posiblemente asociado al consumo de alimentos fibrosos y a la fuerza imprimida en la acción masticatoria, además es agravado por partículas abrasivas que se adherían a los alimentos, durante la maceración con elementos líticos. Las estructuras dentales más afectadas por el desgaste dental corresponden al esmalte y

  14. Bone marrow infection with mycobacterium fortuitum in a diabetic patient

    International Nuclear Information System (INIS)

    Satti, L.; Abbasi, S.; Sattar, A.; Ikram, A.; Manzar, M.A.; Khalid, M.M.

    2011-01-01

    Incidence and prevalence of Mycobacterium fortuitum infection vary greatly by location and death is very rare except in disseminated disease in immunocompromised individuals. We present what we believe is the first case of bone marrow infection with Mycobacterium fortuitum in an HIV negative patient. Bone marrow examination revealed presence of numerous acid fast bacilli which were confirmed as Mycobacterium fortuitum on culture and by molecular analysis. Patient was managed successfully with amikacin and ciprofloxacin. (author)

  15. Seguridad en espacios confinados aplicados en El Complejo Metalúrgico de La Oroya

    OpenAIRE

    Álvarez Valencia, Enrique Camilo; Álvarez Valencia, Enrique Camilo

    2010-01-01

    El tema está referido al conocimiento y análisis profundo de los peligros y riesgos que están asociados a los espacios confinados cuyas principales características de: “falta de oxígeno, poca ventilación y presencia de gases tóxicos y atmósferas explosivas entre otras”, están presentes en las actividades cotidianas del hogar, la comunidad, y la industria en general. En este contexto, las actividades minero metalúrgicas tienen un alto inventario de trabajos con estas características, debido a ...

  16. Modelo POE en la simulación por computador de sistemas complejos

    Directory of Open Access Journals (Sweden)

    Jorge Eduardo Ortiz Triviño

    1999-07-01

    La idea central es simular características de los seres vivos, tales como su capacidad de decisión y acoplamiento al entorno. Con este fin surgió el MODELO POE, que integra tres características biológicas interesantes: Philogenia (evolución, Ontogenia (diversidad y especialización, y Epigenia (aprendizaje. En este artículo se explica el surgimiento y la filosofía del MODELO POE y su aplicación directa en ciencias de la computación.

  17. El metabolismo del colesterol: cada vez más complejo

    Directory of Open Access Journals (Sweden)

    Sanhueza, J.

    2012-10-01

    Full Text Available Cholesterol is an important molecule; it is necessary for the biosynthesis of steroidal hormones, bile salts and to maintain the stability of biological membranes in animal cells. However, its excess is negative and is responsible for the development of many diseases involving the heart and brain, or in the generation of some types of cancer. For these reasons, the cellular cholesterol levels must be finely regulated and therefore, an infinite number of mechanisms participate in this regulation, which undertake the organism as a whole. These mechanisms should begin to operate efficiently from the intake of cholesterol from the diet, its incorporation into the enterocyte, where are involved carriers such as ABC and NCP1 transporters, PDZ structural motif, to name a few. It is also necessary an adequate regulation of circulating cholesterol and once inside the body, there should be a perfect harmony between the addition of cholesterol to various tissues, its metabolic use, the mechanisms of its tissue deposition, and the synthesis of this lipid. From this perspective, this review offers a general view of the molecular mechanisms that allow the regulation of extra and intracellular cholesterol levels.

    El colesterol es una molécula importante; es necesario para la biosíntesis de hormonas esteroideas, de sales biliares y para mantener la estabilidad de las membranas biológicas en células animales. Sin embargo, su exceso es negativo y es responsable de generar enfermedades que comprometen al corazón, al cerebro o que generan algunos tipos de cáncer. Por estos motivos, los niveles de colesterol celulares deben estar finamente regulados, y para ello, participan una infinidad de mecanismos que comprometen al organismo como un todo. Estos mecanismos deben comenzar a operar en forma eficiente desde la ingesta de colesterol dietario para su incorporación al enterocito, donde están implicados transportadores de tipo ABC y NCP1, los motivos

  18. Energy efficiency: a necessity in complex surroundings; Eficiencia energetica: una necesidad en un entorno complejo

    Energy Technology Data Exchange (ETDEWEB)

    Menendez Perez, E. [Alcion Ingenieria Quimica, S.A. (Spain)

    2005-10-15

    A global analysis of the energy system with reference to the energy case of Spain in comparison to the yield of the European Union is proposed. The analysis discusses the problems originated by the climatic change and exhibits three decisive concepts in energy efficiency (investment, good use and productivity) along with three indicating phenomena of an efficient or incompetent development (mobility, tourism and urbanism). Next, it exemplifies possible evolution criteria of the electrical production systems along with an ideal of evolution in the generation systems simultaneously mentioning measures for the emissions reduction in the industry, particularly in the automotive industry. In addition, it considers the imposition of energy taxes in front of the non fulfillment of the emissions' control as an important restrictive measurement. In between, the solution initiatives that have had a short reach, such as the Kyoto protocol, and categorical conclusions are contemplated: The cleanest energy is the one than is not consumed, not because it is not available but because it is not necessary, therefore it is not demanded. [Spanish] Se propone un analisis global del sistema de energia con referencia al caso energetico de Espana en equiparacion al rendimiento de la Union Europea. El analisis discute las problematicas originadas por el cambio climatico y exhibe tres conceptos decisivos en la eficiencia energetica (inversion, buen uso y productividad) junto con tres fenomenos indicadores de un desarrollo eficiente o incompetente (movilidad, turismo y urbanismo). Enseguida ejemplifica posibles criterios de evolucion de los sistemas de produccion electrica junto con un ideal de evolucion en los sistemas de generacion, a la vez que cita medidas para la reduccion de emisiones en la industria, particularmente en la industria automotriz. Se considera la imposicion de impuestos energeticos frente al incumplimiento en el control de emisiones como una importante medida

  19. Mycobacterium komaniense sp. nov., a rapidly growing non-tuberculous Mycobacterium species detected in South Africa.

    Science.gov (United States)

    Gcebe, Nomakorinte; Rutten, Victor P M G; van Pittius, Nicolaas Gey; Naicker, Brendon; Michel, Anita L

    2018-05-01

    Some species of non-tuberculous mycobacteria (NTM) have been reported to be opportunistic pathogens of animals and humans. Recently there has been an upsurge in the number of cases of NTM infections, such that some NTM species are now recognized as pathogens of humans and animals. From a veterinary point of view, the major significance of NTM is the cross-reactive immune response they elicit against Mycobacterium bovis antigens, leading to misdiagnosis of bovine tuberculosis. Four NTM isolates were detected from a bovine nasal swab, soil and water, during an NTM survey in South Africa. These were all found using 16S rRNA gene sequence analysis to be closely related to Mycobacterium moriokaense. The isolates were further characterised by sequence analysis of the partial fragments of hsp65, rpoB and sodA. The genome of the type strain was also elucidated. Gene (16S rRNA, hsp65, rpoB and sodA) and protein sequence data analysis of 6 kDa early secretory antigenic target (ESAT 6) and 10 kDa culture filtrate protein (CFP-10) revealed that these isolates belong to a unique Mycobacterium species. Differences in phenotypic and biochemical traits between the isolates and closely related species further supported that these isolates belong to novel Mycobacterium species. We proposed the name Mycobacterium komaniense sp. nov. for this new species. The type strain is GPK 1020 T (=CIP 110823T=ATCC BAA-2758).

  20. Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex.

    Science.gov (United States)

    Bouam, Amar; Heidarieh, Parvin; Shahraki, Abodolrazagh Hashemi; Pourahmad, Fazel; Mirsaeidi, Mehdi; Hashemzadeh, Mohamad; Baptiste, Emeline; Armstrong, Nicholas; Levasseur, Anthony; Robert, Catherine; Drancourt, Michel

    2018-03-07

    Four slowly growing mycobacteria isolates were isolated from the respiratory tract and soft tissue biopsies collected in four unrelated patients in Iran. Conventional phenotypic tests indicated that these four isolates were identical to Mycobacterium lentiflavum while 16S rRNA gene sequencing yielded a unique sequence separated from that of M. lentiflavum. One representative strain AFP-003 T was characterized as comprising a 6,121,237-bp chromosome (66.24% guanosine-cytosine content) encoding for 5,758 protein-coding genes, 50 tRNA and one complete rRNA operon. A total of 2,876 proteins were found to be associated with the mobilome, including 195 phage proteins. A total of 1,235 proteins were found to be associated with virulence and 96 with toxin/antitoxin systems. The genome of AFP-003 T has the genetic potential to produce secondary metabolites, with 39 genes found to be associated with polyketide synthases and non-ribosomal peptide syntases and 11 genes encoding for bacteriocins. Two regions encoding putative prophages and three OriC regions separated by the dnaA gene were predicted. Strain AFP-003 T genome exhibits 86% average nucleotide identity with Mycobacterium genavense genome. Genetic and genomic data indicate that strain AFP-003 T is representative of a novel Mycobacterium species that we named Mycobacterium ahvazicum, the nineteenth species of the expanding Mycobacterium simiae complex.

  1. Mycobacterium bovis and Other Uncommon Members of the Mycobacterium tuberculosis Complex.

    Science.gov (United States)

    Esteban, Jaime; Muñoz-Egea, Maria-Carmen

    2016-12-01

    Since its discovery by Theobald Smith, Mycobacterium bovis has been a human pathogen closely related to animal disease. At present, M. bovis tuberculosis is still a problem of importance in many countries and is considered the main cause of zoonotic tuberculosis throughout the world. Recent development of molecular epidemiological tools has helped us to improve our knowledge about transmission patterns of this organism, which causes a disease indistinguishable from that caused by Mycobacterium tuberculosis. Diagnosis and treatment of this mycobacterium are similar to those for conventional tuberculosis, with the important exceptions of constitutive resistance to pyrazinamide and the fact that multidrug-resistant and extremely drug-resistant M. bovis strains have been described. Among other members of this complex, Mycobacterium africanum is the cause of many cases of tuberculosis in West Africa and can be found in other areas mainly in association with immigration. M. bovis BCG is the currently available vaccine for tuberculosis, but it can cause disease in some patients. Other members of the M. tuberculosis complex are mainly animal pathogens with only exceptional cases of human disease, and there are even some strains, like "Mycobacterium canettii," which is a rare human pathogen that could have an important role in the knowledge of the evolution of tuberculosis in the history.

  2. 21 CFR 866.3370 - Mycobacterium tuberculosis immunofluorescent reagents.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents... used to identify Mycobacterium tuberculosis directly from clinical specimens. The identification aids...

  3. A single or multistage mycobacterium avium subsp. paratuberculosis subunit vaccine

    DEFF Research Database (Denmark)

    2014-01-01

    The present invention provides one or more immunogenic polypeptides for use in a preventive or therapeutic vaccine against latent or active infection in a human or animal caused by a Mycobacterium species, e.g. Mycobacterium avium subsp. paratuberculosis. Furthermore a single or multi-phase vaccine...... comprising the one or more immunogenic polypeptides is provided for administration for the prevention or treatment of infection with a Mycobacterium species, e.g. Mycobacterium avium subsp. paratuberculosis. Additionally, nucleic acid vaccines, capable of in vivo expression of the multi-phase vaccine...

  4. Aspectos relevantes del uso de Mycobacterium´habana´ como candidato vacunal contra la tuberculosis

    Directory of Open Access Journals (Sweden)

    Iliana Valdés

    2011-12-01

    Full Text Available La eficacia protectora de la actual vacuna (BCG contra la tuberculosis, para contrarrestar las formas pulmonares de esta enfermedad y su reactivación, resulta variable o poco eficiente, lo cual impone la búsqueda urgente de nuevas alternativas profilácticas contra esta enfermedad. Basados en las ventajas inmunogénicas que ofrece el uso de vacunas vivas, se han encaminado diferentes estrategias de este tipo empleando mutantes auxotróficos de Mycobacterium tuberculosis , BCG recombinante o micobacterias no tuberculosas. Existen evidencias experimentales acerca de la protección conferida tras la vacunación con cepas vivas, inactivadas o fracciones proteicas de Mycobacterium'habana' TMC-5135 contra la infección por Mycobacterium tuberculosis. Esta respuesta protectora parece ir aparejada de escasos signos de virulencia en los modelos animales ensayados, lo cual coloca a M.´habana´ dentro de los posibles candidatos vacunales contra la tuberculosis al ajustarse a la condición que impone una vacuna clásica de reproducir la infección y los eventos inmunes que le suceden lo más fielmente posible a como ocurren de manera natural, sin causar extensos daños en el receptor.

  5. Infection caused by Mycobacterium tuberculosis.

    Science.gov (United States)

    Peloquin, C A; Berning, S E

    1994-01-01

    To update readers on the clinical management of infections caused by Mycobacterium tuberculosis, to provide a general description of the organism, culture and susceptibility testing, and clinical manifestations of the disease, and to provide several aspects of the treatment of the disease, including historical perspective, current approaches, and research opportunities for the future. The current medical literature, including abstracts presented at recent international meetings, is reviewed. References were identified through MEDLINE, MEDLARS II, Current Contents, and published meeting abstracts. Data regarding the epidemiology, clinical manifestations, culture and susceptibility testing, and treatment of tuberculosis are cited. Specific attention has been focused on the clinical management of patients with noncontagious infection and potentially contagious active disease (TB) caused by M. tuberculosis. Information contributing to the discussion of the topics selected by the authors is reviewed. Data supporting and disputing specific conclusions are presented. The incidence of TB is increasing in the US, despite the fact that available technologies are capable of controlling the vast majority of existing cases. Fueling the fire is the problem of coinfection with HIV and M. tuberculosis. Very few drugs are available for the treatment of TB, and few of these approach the potency of isoniazid and rifampin. Preventive therapy of patients exposed to multiple-drug-resistant M. tuberculosis (MDR-TB) is controversial and of unknown efficacy. Treatment of active disease caused by MDR-TB requires up to four times longer, is associated with increased toxicity, and is far less successful than the treatment of drug-susceptible TB. Strategies for the management of such cases are presented. The rising incidence of TB in the US reflects a breakdown in the healthcare systems responsible for controlling the disease, which reflects the past budgetary reductions. Although TB control

  6. [Identification and drug susceptibility testing of Mycobacterium thermoresistibile and Mycobacterium elephantis isolated from a cow with mastitis].

    Science.gov (United States)

    Li, W B; Ji, L Y; Xu, D L; Liu, H C; Zhao, X Q; Wu, Y M; Wan, K L

    2018-05-10

    Objective: To understand the etiological characteristics and drug susceptibility of Mycobacterium thermoresistibile and Mycobacterium elephantis isolated from a cow with mastitis and provide evidence for the prevention and control of infectious mastitis in cows. Methods: The milk sample was collected from a cow with mastitis, which was pretreated with 4 % NaOH and inoculated with L-J medium for Mycobacterium isolation. The positive cultures were initially identified by acid-fast staining and multi-loci PCR, then Mycobacterium species was identified by the multiple loci sequence analysis (MLSA) with 16S rRNA , hsp65 , ITS and SodA genes. The drug sensitivity of the isolates to 27 antibiotics was tested by alamar blue assay. Results: Two anti-acid stain positive strains were isolated from the milk of a cow with mastitis, which were identified as non- tuberculosis mycobacterium by multi-loci PCR, and multi-loci nucleic acid sequence analysis indicated that one strain was Mycobacterium thermoresistibile and another one was Mycobacterium elephantis . The results of the drug susceptibility test showed that the two strains were resistant to most antibiotics, including rifampicin and isoniazid, but they were sensitive to amikacin, moxifloxacin, levofloxacin, ethambutol, streptomycin, tobramycin, ciprofloxacin and linezolid. Conclusions: Mycobacterium thermoresistibile and Mycobacterium elephantis were isolated in a cow with mastitis and the drug susceptibility spectrum of the pathogens were unique. The results of the study can be used as reference for the prevention and control the infection in cows.

  7. Multifaceted role of lipids in Mycobacterium leprae.

    Science.gov (United States)

    Kaur, Gurkamaljit; Kaur, Jagdeep

    2017-03-01

    Mycobacterium leprae must adopt a metabolic strategy and undergo various metabolic alterations upon infection to survive inside the human body for years in a dormant state. A change in lipid homeostasis upon infection is highly pronounced in Mycobacterium leprae. Lipids play an essential role in the survival and pathogenesis of mycobacteria. Lipids are present in several forms and serve multiple roles from being a source of nutrition, providing rigidity, evading the host immune response to serving as virulence factors, etc. The synthesis and degradation of lipids is a highly regulated process and is the key to future drug designing and diagnosis for mycobacteria. In the current review, an account of the distinct roles served by lipids, the mechanism of their synthesis and degradation has been elucidated.

  8. Mineralogía y condiciones de cristalización en el complejo subvolcánico de Barcarrota (Badajoz, España

    Directory of Open Access Journals (Sweden)

    Casquet, C.

    1987-08-01

    Full Text Available A description of mineral chemistry from rocks of the subvolcanic Barcarrota Complex is made. This igneous massif consists of several sub-alkaline lithologies, namely olivine gabbros, diorites, quartz-monzonites and amphibole granites, as well as minor peralkaline granites. Physico-chemical conditions during magma crystallization are obtained on the basis of mineral data. Thus different geothermometers lead to the following crystallization temperatures: olivine gabbros (972-1.008° C, diorites (953-995° C, quartz-monzonites (801-833° C and amphibole granites 750° C. The presence of illmenite as the sole Fe-Ti mineral in these rocks (except in the peralkaline types where it is magnetite, sugests that the fO2 was never higher than the MW buffer, decreasing continuously with the differentiation degree fram fO2= 1O-13.2 atm. for the olivine gabbros to 10-18.87 atm. in the granites Furthemore water played an important role during crystallization of the different magmas.Se estudian las características químicas de los minerales más significativos del Complejo subvolcánico de Barcarrota, formado por litologías subalcalinas consistentes en: gabros olivínicos, dioritas, cuarzo-monzonitas y granitos anfibólicos, así como por pequeños afloramientos de granitos peralcalinos. Estos datos se emplean para definir las condiciones físico-químicas de cristalización magmática. Mediante distintos geotermómetros se obtienen las siguientes temperaturas de cristalización: Gabras olivínicos (972-1008º C, Dioritas (953-995º C, Cuarzo-monzonitas (801-833º C y los Granitos anfibólicos 750º C. La presencia como único óxido de FeTi, de ilmenita en todas las rocas excepto en los tipos peralcalinos, que es magnetita, indica que la fO2 no superó las condiciones del tampón MW, decreciendo con el grado de diferenciación (fO2= 10-132 en los gabros olivínicos hasta 10-18,87 en los granitos. La presencia de agua debió jugar un notable

  9. CAMBIO Estacional EN EL USO DE LOS RECURSOS DE LA COMUNIDAD de reptiles en EL COMPLEJO Cenagoso DE ZAPATOSA, DEPARTAMENTO DEL CESAR (COLOMBIA

    Directory of Open Access Journals (Sweden)

    Medina-Rangel Guido Fabián

    2013-07-01

    Full Text Available Para estudiar cómo los ciclos estacionales influyen en el uso y la agregación de lasespecies de reptiles en los diferentes hábitats, microhábitats y horas de actividad diaria.Se realizaron cuatro salidas de campo en áreas circundantes al complejo cenagoso deZapatosa, entre noviembre de 2006 y octubre de 2007, abarcando la época de lluvias(noviembre de 2006 y octubre de 2007 y la época seca (enero de 2007 y julio de 2007.En total se invirtieron 640 horas/hombre de muestreo, repartidas en cinco hábitats:bosque casmófito, bosque seco, bosque de ribera, palmar y sabana arbolada. Seencontraron 468 individuos distribuidos en 36 especies en época de lluvias y en épocaseca se hallaron 344 individuos repartidos en 35 especies. En época de lluvias el hábitatmás rico en especies fue el bosque casmófito y el más abundante en individuos fue elbosque seco; en época seca el bosque de ribera fue el que presentó el mayor númerode especies e individuos. El mayor recambio temporal de especies dentro del mismohábitat lo presentó la sabana arbolada. Las especies que dominaron la comunidaden ambos periodos climáticos fueron Gonatodes albogularis, Anolis tropidogastery Anolis auratus. La disimilitud en cuanto a las abundancias y la composición delas especies que se presentó entre épocas climáticas fue del 34% y las especies quemás contribuyeron a esto fueron Ameiva praesignis, Mabuya sp. y Holcosus festivus.Las horas de actividad de un período climático a otro fueron similares. Los lagartosmostraron mayor amplitud y las serpientes más alta superposición en el uso del tiempode actividad en los dos periodos climático. La amplitud en el uso de los recursos nopresentó un patrón generalizado por grupos de reptiles de un periodo a otro, ni resultódiferente estadísticamente entre época climática. La superposición en el uso de losrecursos fue mayor en la época seca frente a la de lluvias.

  10. Nitazoxanide is active against Mycobacterium leprae

    Science.gov (United States)

    Bailey, Mai Ann; Na, Hana; Duthie, Malcolm S.; Gillis, Thomas P.; Lahiri, Ramanuj

    2017-01-01

    Nitazoxanide (NTZ) is an anti-parasitic drug that also has activity against bacteria, including Mycobacterium tuberculosis. Our data using both radiorespirometry and live-dead staining in vitro demonstrate that NTZ similarly has bactericidal against M. leprae. Further, gavage of M. leprae-infected mice with NTZ at 25mg/kg provided anti-mycobacterial activity equivalent to rifampicin (RIF) at 10 mg/kg. This suggests that NTZ could be considered for leprosy treatment. PMID:28850614

  11. Comparative Mycobacteriology of the Mycobacterium tuberculosis complex

    OpenAIRE

    Gordon, Stephen V.; Behr, Marcel A.

    2015-01-01

    The Mycobacterium tuberculosis complex (MTBC) is a group of highly genetically related pathogens that cause tuberculosis (TB) in mammalian species. However, the very name of the complex underlines the fact that our knowledge of these pathogens is dominated by studies on the human pathogen, M. tuberculosis. Of course this is entirely justified; M. tuberculosis is a major global pathogen that exacts a horrendous burden in terms of mortality and morbidity so it is appropriate that it is...

  12. Inactivation of Mycobacterium paratuberculosis and Mycobacterium tuberculosis in fresh soft cheese by gamma radiation

    International Nuclear Information System (INIS)

    Badr, Hesham M.

    2011-01-01

    The effectiveness of gamma irradiation on the inactivation of Mycobacterium paratuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis in fresh soft cheese that prepared from artificially inoculated milk samples was studied. Irradiation at dose of 2 kGy was sufficient for the complete inactivation of these mycobacteria as they were not detected in the treated samples during storage at 4±1 o C for 15 days. Moreover, irradiation of cheese samples, that were prepared from un-inoculated milk, at this effective dose had no significant effects on their gross composition and contents from riboflavin, niacin and pantothenic acid, while significant decreases in vitamin A and thiamin were observed. In addition, irradiation of cheese samples had no significant effects on their pH and nitrogen fractions contents, except for the contents of ammonia, which showed a slight, but significant, increases due to irradiation. The analysis of cheese fats indicated that irradiation treatment induced significant increase in their oxidation parameters and contents from free fatty acids; however, the observed increases were relatively low. On the other hand, irradiation of cheese samples induced no significant alterations on their sensory properties. Thus, irradiation dose of 2 kGy can be effectively applied to ensure the safety of soft cheese with regards to these harmful mycobacteria. - Highlights: → We examined the effectiveness of gamma irradiation on inactivation of Mycobacterium paratuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis in fresh soft cheese. → Irradiation at dose of 2 kGy was sufficient for complete inactivation of these mycobacteria. → Irradiation of cheese samples induced no significant alterations on their sensory properties.

  13. Inactivation of Mycobacterium paratuberculosis and Mycobacterium tuberculosis in fresh soft cheese by gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Badr, Hesham M., E-mail: heshambadr_aea@yahoo.co.uk [Atomic Energy Authority, Nuclear Research Center, Abou Zaabal, P.O. Box 13759 Cairo (Egypt)

    2011-11-15

    The effectiveness of gamma irradiation on the inactivation of Mycobacterium paratuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis in fresh soft cheese that prepared from artificially inoculated milk samples was studied. Irradiation at dose of 2 kGy was sufficient for the complete inactivation of these mycobacteria as they were not detected in the treated samples during storage at 4{+-}1 {sup o}C for 15 days. Moreover, irradiation of cheese samples, that were prepared from un-inoculated milk, at this effective dose had no significant effects on their gross composition and contents from riboflavin, niacin and pantothenic acid, while significant decreases in vitamin A and thiamin were observed. In addition, irradiation of cheese samples had no significant effects on their pH and nitrogen fractions contents, except for the contents of ammonia, which showed a slight, but significant, increases due to irradiation. The analysis of cheese fats indicated that irradiation treatment induced significant increase in their oxidation parameters and contents from free fatty acids; however, the observed increases were relatively low. On the other hand, irradiation of cheese samples induced no significant alterations on their sensory properties. Thus, irradiation dose of 2 kGy can be effectively applied to ensure the safety of soft cheese with regards to these harmful mycobacteria. - Highlights: > We examined the effectiveness of gamma irradiation on inactivation of Mycobacterium paratuberculosis, Mycobacterium bovis and Mycobacterium tuberculosis in fresh soft cheese. > Irradiation at dose of 2 kGy was sufficient for complete inactivation of these mycobacteria. > Irradiation of cheese samples induced no significant alterations on their sensory properties.

  14. A Dermal Piercing Complicated by Mycobacterium fortuitum

    Science.gov (United States)

    Scroggins-Markle, Leslie; Kelly, Brent

    2013-01-01

    Background. Dermal piercings have recently become a fashion symbol. Common complications include hypertrophic scarring, rejection, local infection, contact allergy, and traumatic tearing. We report a rare case of Mycobacterium fortuitum following a dermal piercing and discuss its medical implications and treatments. Case. A previously healthy 19-year-old woman presented complaining of erythema and edema at the site of a dermal piercing on the right fourth dorsal finger. She was treated with a 10-day course of trimethoprim-sulfamethoxazole and one course of cephalexin by her primary care physician with incomplete resolution. The patient stated that she had been swimming at a local water park daily. A punch biopsy around the dermal stud was performed, and cultures with sensitivities revealed Mycobacterium fortuitum. The patient was treated with clarithromycin and ciprofloxacin for two months receiving full resolution. Discussion. Mycobacterium fortuitum is an infrequent human pathogen. This organism is a Runyon group IV, rapidly growing nontuberculous mycobacteria, often found in water,soil, and dust. Treatment options vary due to the size of the lesion. Small lesions are typically excised, while larger lesions require treatment for 2–6 months with antibiotics. We recommend a high level of suspicion for atypical mycobacterial infections in a piercing resistant to other therapies. PMID:24073343

  15. A Dermal Piercing Complicated by Mycobacterium fortuitum

    Directory of Open Access Journals (Sweden)

    Trisha Patel

    2013-01-01

    Full Text Available Background. Dermal piercings have recently become a fashion symbol. Common complications include hypertrophic scarring, rejection, local infection, contact allergy, and traumatic tearing. We report a rare case of Mycobacterium fortuitum following a dermal piercing and discuss its medical implications and treatments. Case. A previously healthy 19-year-old woman presented complaining of erythema and edema at the site of a dermal piercing on the right fourth dorsal finger. She was treated with a 10-day course of trimethoprim-sulfamethoxazole and one course of cephalexin by her primary care physician with incomplete resolution. The patient stated that she had been swimming at a local water park daily. A punch biopsy around the dermal stud was performed, and cultures with sensitivities revealed Mycobacterium fortuitum. The patient was treated with clarithromycin and ciprofloxacin for two months receiving full resolution. Discussion. Mycobacterium fortuitum is an infrequent human pathogen. This organism is a Runyon group IV, rapidly growing nontuberculous mycobacteria, often found in water,soil, and dust. Treatment options vary due to the size of the lesion. Small lesions are typically excised, while larger lesions require treatment for 2–6 months with antibiotics. We recommend a high level of suspicion for atypical mycobacterial infections in a piercing resistant to other therapies.

  16. Phylogenetic analysis of vitamin B12-related metabolism in Mycobacterium tuberculosis

    OpenAIRE

    Young, Douglas B.; Comas, I?aki; de Carvalho, Luiz P. S.

    2015-01-01

    Comparison of genome sequences from clinical isolates of Mycobacterium tuberculosis with phylogenetically-related pathogens Mycobacterium marinum, Mycobacterium kansasii, and Mycobacterium leprae reveals diversity amongst genes associated with vitamin B12-related metabolism. Diversity is generated by gene deletion events, differential acquisition of genes by horizontal transfer, and single nucleotide polymorphisms (SNPs) with predicted impact on protein function and transcriptional regulation...

  17. Mycobacterium marinum infections in Denmark from 2004 to 2017

    DEFF Research Database (Denmark)

    Holden, Inge K.; Kehrer, Michala; Andersen, Aase B.

    2018-01-01

    Mycobacterium marinum (M. marinum) is a slowly growing nontuberculous mycobacterium. The incidence of M. marinum infections in Denmark is unknown. We conducted a retrospective nationwide study including all culture confirmed cases of M. marinum from 2004 to 2017 in Denmark. All available medical ...

  18. Complete Genome Sequence of Mycobacterium vaccae Type Strain ATCC 25954

    KAUST Repository

    Ho, Y. S.; Adroub, S. A.; Abadi, Maram; Al Alwan, B.; Alkhateeb, R.; Gao, G.; Ragab, A.; Ali, Shahjahan; van Soolingen, D.; Bitter, W.; Pain, Arnab; Abdallah, A. M.

    2012-01-01

    Mycobacterium vaccae is a rapidly growing, nontuberculous Mycobacterium species that is generally not considered a human pathogen and is of major pharmaceutical interest as an immunotherapeutic agent. We report here the annotated genome sequence of the M. vaccae type strain, ATCC 25954.

  19. Mycobacterium marinum kan være vanskelig at diagnosticere

    DEFF Research Database (Denmark)

    Lønnberg, Ann Sophie; Seersholm, Niels; Nielsen, Signe Ledou

    2012-01-01

    The diagnosis of cutaneous Mycobacterium marinum infection is often delayed for months after presentation. In this case the diagnosis and correct treatment was delayed for ten months resulting in possible irreversible damage to the patient's infected finger. The main reason for the delay is lack...... of knowledge of the mycobacterium....

  20. Complete Genome Sequence of Mycobacterium vaccae Type Strain ATCC 25954

    KAUST Repository

    Ho, Y. S.

    2012-10-26

    Mycobacterium vaccae is a rapidly growing, nontuberculous Mycobacterium species that is generally not considered a human pathogen and is of major pharmaceutical interest as an immunotherapeutic agent. We report here the annotated genome sequence of the M. vaccae type strain, ATCC 25954.

  1. An orphan gyrB in the Mycobacterium smegmatis genome

    Indian Academy of Sciences (India)

    DNA gyrase is an essential topoisomerase found in all bacteria. It is encoded by gyrB and gyrA genes. These genes are organized differently in different bacteria. Direct comparison of Mycobacterium tuberculosis and Mycobacterium smegmatis genomes reveals presence of an additional gyrB in M. smegmatis flanked by ...

  2. Risk factors for Mycobacterium tuberculosis infection among children in Greenland

    DEFF Research Database (Denmark)

    Søborg, Bolette; Andersen, Aase Bengaard; Melbye, Mads

    2011-01-01

    To examine the risk factors for Mycobacterium tuberculosis infection (MTI) among Greenlandic children for the purpose of identifying those at highest risk of infection.......To examine the risk factors for Mycobacterium tuberculosis infection (MTI) among Greenlandic children for the purpose of identifying those at highest risk of infection....

  3. Draft Genome Sequence of Mycobacterium chimaera Type Strain Fl-0169

    Science.gov (United States)

    We report the draft genome sequence of the type strain Mycobacterium chimaera Fl-0169T, a member of the Mycobacterium avium complex (MAC). M. chimaera Fl-0169T was isolated from a patient in Italy and is highly similar to strains of M. chimaera isolated in Ireland, though Fl-016...

  4. EFECTO DE UN COMPLEJO DE VITAMINAS Y AMINOÁCIDOS EN EL COMPORTAMIENTO PRODUCTIVO DE GALLINAS PONEDORAS

    Directory of Open Access Journals (Sweden)

    MÓNICA MARÍA ESTRADA P.

    Full Text Available Los sistemas intensivos de producción de huevo comercial pueden conllevar a las aves a un estado de inmunodepresión, esto puede ser mitigado con el suministro de nutrientes que activen los mecanismos fisiológicos del animal. Este trabajo evaluó el efecto de un complejo de vitaminas y aminoácidos en el desempeño productivo de gallinas ponedoras. Un lote de 20395 gallinas de la línea Lohmann LSL, se dividió en dos grupos, el primer grupo recibió el tratamiento de vitaminas y aminoácidos (T2, el segundo grupo se estableció como el control (T1. Se empleó la estadística de T2 de Hotelling, se utilizó el método de Spearman para la determinación de la relación entre variables donde el análisis se suplementó por medio de la técnica descriptiva unidireccional. Para las variables productivas consumo de alimento, porcentaje de producción de huevos, huevo ave alojada, viabilidad y conversión alimenticia se encontró diferencias estadísticas altamente significativas (p<0,0001 en el efecto promedio de los grupos tomando en forma simultánea todas las variables respuesta. El grupo (T2 presentó mejor desempeño productivo.

  5. A PULMONARY INFECTION CAUSED BY MYCOBACTERIUM PEREGRINUM– A CASE REPORT.

    Directory of Open Access Journals (Sweden)

    Tatina T. Todorova

    2015-12-01

    Full Text Available Mycobacterium peregrinum is a member of the group of rapidly growing Nontuberculous Mycobacteria (NTM. It can be found in high frequency in natural and laboratory environments and is considered to be uncommonrare pathogen for both immunocompetent and immunosuppressed individuals. Currently, pulmonary infections caused by Mycobacterium peregrinum are unusual and diagnosed only in limited number of cases. Here, we present a clinical case of elderly man (72 years with 1 month history of non-specific respiratory symptomatic. The patient was without underlying immunosuppressive condition or lung disease. Chest X-ray demonstrated persistent pleural effusion, opacities and cavitations in the right lobe. One of the sputum culturesgrewa rapidly growing mycobacterium and the isolated strain was found to be Mycobacterium peregrinumas identified by molecular genetic detection (PCR and DNA strip technology. To our knowledge, this is the third case in the world to report Mycobacterium peregrinumas a possible causative agent of pulmonary infection.

  6. Mycobacterium malmesburyense sp. nov., a non-tuberculous species of the genus Mycobacterium revealed by multiple gene sequence characterization

    CSIR Research Space (South Africa)

    Gcebe, N

    2017-04-01

    Full Text Available Journal of Systematic and Evolutionary Microbiology: DOI 10.1099/ijsem.0.001678 Mycobacterium malmesburyense sp. nov., a non-tuberculous species of the genus Mycobacterium revealed by multiple gene sequence characterization Gcebe N Rutten V Gey...

  7. Immunological crossreactivity of the Mycobacterium leprae CFP-10 with its homologue in Mycobacterium tuberculosis

    NARCIS (Netherlands)

    Geluk, A.; van Meijgaarden, K. E.; Franken, K. L. M. C.; Wieles, B.; Arend, S. M.; Faber, W. R.; Naafs, B.; Ottenhoff, T. H. M.

    2004-01-01

    Mycobacterium tuberculosis culture filtrate protein-10 (CFP-10) (Rv3874) is considered a promising antigen for the immunodiagnosis of tuberculosis (TB) together with early secreted antigens of M. tuberculosis (ESAT-6). Both ESAT-6 and CFP-10 are encoded by the RD1 region that is deleted from all

  8. Bacteriological diagnosis and molecular strain typing of Mycobacterium bovis and Mycobacterium caprae.

    Science.gov (United States)

    Gormley, E; Corner, L A L; Costello, E; Rodriguez-Campos, S

    2014-10-01

    The primary isolation of a Mycobacterium sp. of the Mycobacterium tuberculosis complex from an infected animal provides a definitive diagnosis of tuberculosis. However, as Mycobacterium bovis and Mycobacterium caprae are difficult to isolate, particularly for animals in the early stages of disease, success is dependent on the optimal performance of all aspects of the bacteriological process, from the initial choice of tissue samples at post-mortem examination or clinical samples, to the type of media and conditions used to cultivate the microorganism. Each step has its own performance characteristics, which can contribute to sensitivity and specificity of the procedure, and may need to be optimized in order to achieve the gold standard diagnosis. Having isolated the slow-growing mycobacteria, species identification and fine resolution strain typing are keys to understanding the epidemiology of the disease and to devise strategies to limit transmission of infection. New technologies have emerged that can now even discriminate different isolates from the same animal. In this review we highlight the key factors that contribute to the accuracy of bacteriological diagnosis of M. bovis and M. caprae, and describe the development of advanced genotyping techniques that are increasingly used in diagnostic laboratories for the purpose of supporting detailed epidemiological investigations. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Predicción del riesgo individual de alto coste sanitario para la identificación de pacientes crónicos complejos

    Directory of Open Access Journals (Sweden)

    Jordi Coderch

    2014-07-01

    Conclusiones: El consumo sanitario elevado se relaciona con la morbilidad crónica compleja. Un modelo basado en la edad, la morbilidad y la utilización previa es válido para predecir el riesgo de alto consumo, y así identificar la población diana de estrategias de atención proactiva para pacientes crónicos complejos.

  10. Gobierno de la información: complejo paradigma de fundamental implementación

    Directory of Open Access Journals (Sweden)

    Nahabetián, Laura

    2014-09-01

    Full Text Available La información es la base de todo gobierno. La información guía las decisiones y procesos por más cortos o largos que éstos sean, desde aquéllos que determinan la guerra o la paz hasta la recolección de residuos. La última década ha sido testigo de una atención sin precedentes a la maquinaria de la información en el gobierno -a menudo categorizada como “gobierno electrónico”-; la pregunta es si esto es correcto. Las tecnologías de la información y comunicación se han promocionado como la solución para todo, desde la rígida arquitectura de gobierno a las tasas de caída de participación en las diferentes democracias del mundo. Con demasiada frecuencia la atención del gobierno electrónico ha sido en la tecnología, en lo técnicamente posible, y no en los flujos de información. Sin embargo, ha devenido trascendente explorar una comprensión más profunda del papel de la información, los flujos dentro del gobierno, entre el gobierno y los ciudadanos, y, en menor medida, entre los ciudadanos con respecto al gobierno. La tecnología sólo puede hacer que ciertos tipos de flujos de información sean posibles; esto no significa, que ella los determine o incluso los produzca. Teniendo en cuenta las tecnologías actuales, la elasticidad de las instituciones humanas y la cognición son mucho menores que la de los bits. El gobierno de la información no es otra etapa del gobierno electrónico; sino que se trata de una especie de lente conceptual que ofrece una perspectiva complementaria para entender la naturaleza cambiante de los gobiernos y su relación con la ciudadanía

  11. ALS Association

    Science.gov (United States)

    ... toward a world without ALS! Walk to Defeat ALS® Walk to Defeat ALS® draws people of all ... We need your help. I Will Advocate National ALS Registry The National ALS Registry is a congressionally ...

  12. Caracterización del complejo productivo de la madera de la Provincia de Córdoba, Argentina. Factores que dificultan su competitividad

    Directory of Open Access Journals (Sweden)

    Gerardo Andrés Denegri

    2016-01-01

    Full Text Available El objetivo del trabajo es determinar y analizar las cadenas foresto-industriales que componen el complejo productivo de la madera de la provincia de Córdoba, y discutir las variables que afectan su competitividad. Para recabar los datos se recurrió a encuestas semiestructuradas a informantes claves y revisión bibliográfica. Se identificaron y analizaron tres cadenas: la del mueble de madera, la de productos para la construcción y la de envases y tarimas. Del análisis del comportamiento de los actores, surge que la rentabilidad de las empresas del eslabón industrial se logra transfiriendo la ineficiencia del complejo a las forestaciones y, en menor medida, a los bosques nativos, maximizando la ganancia individual. Se sostiene como conclusión que sin la intervención de diferentes niveles del Estado, la posibilidad de obtener una cierta rentabilidad de corto plazo y una fuerte cultura individualista no son incentivos para cooperar y generar procesos sostenibles de integración. La cooperación entre los distintos actores facilitaría una mayor integración entre las cadenas, la incorporación de valor, elevar la eficiencia y la competitividad sistémica del complejo.

  13. SÍNTESIS Y CARACTERIZACIÓN DE UN CUADRADO SUPRAMOLECULARA PARTIR DEL AUTO-ENSAMBLAJE DEL COMPLEJO[1,2-BIS(DIFENILFOSFINOETANO] BISTRIFLUOROMETANOSULFONATOPALADIO (II Y 4,4´-BIPIRIDINA

    Directory of Open Access Journals (Sweden)

    Julian Posada

    2011-11-01

    Full Text Available Por primera vez se desarrolló la síntesisy caracterización (IR, UV, RMN-1H,31P, 19F de un nuevo cuadrado supramolecular[3] mediante el auto-ensamblajedel complejo [Pd(CF3SO32(Dppe] [1] yel ligando orgánico 4,4´-Bipiridina [2].Se evidenció la obtención de [3] en mezclacon otra especie supramolecular [4],asignada como un macrociclo triangular.Las estructuras de [3] y [4] se elucidaroncon base en los desplazamientos químicos,la proporcionalidad entre sus integralesy en los patrones de acoplamientode las señales en los espectros RMN-1H yCOSY 1H-1H. Mediante la relación entrelas integrales de las señales correspondientesa las especies supramolecularescoexistentes en los espectros RMN-1Hy 31P se determinó la composición de lamezcla al final de la reacción conteniendo70 % de [3] y 30 % de [4].

  14. Alelos del complejo mayor de histocompatibilidad BoLA clase II asociados a la resistencia y susceptibilidad a Boophilus microplus en ganado bovino, Junín–Perú

    Directory of Open Access Journals (Sweden)

    M. Custodio Villanueva

    2011-01-01

    Full Text Available El objetivo del presente estudio fue identificar mediante la amplificación de microsatélites, los alelos del Complejo Mayor de Histocompatibilidad (CMH BoLA Clase II aso ciados a la resistencia y susceptibilidad a Boophilus microplus en ganado bovino, Junín – Perú. S e recolectaron muestra s de sangre de 47 bovinos , a sí como, ejemplares de B. microplus durante los meses de marzo, abril y mayo del 2009, en horas de la mañana, del lado derecho de cada animal. La extracción del ADN genómico se realizó por el método de Phil Summers (1995 y la amplificación de los microsatélites DRB3.2, DRB31 y BM1815 fue por PCR en un secuenciador automático. Los datos fueron evaluados mediante e l programa POPGEN 32. Se identificaron los alelos A, B y C relacionados con la resistencia y susceptibilidad a B. microplus en ganado bovino tanto en el locus BoLA DRB3.2 como en BoLA BM1815. Las frecuencias alélicas estimadas fueron: en el locus DRB3.2 la frecuencia del alelo A fue de 0 . 2742, del alelo B fue de 0.5000 y del alelo C fue de 0.2258; en el locus DRB31 la frecuencia del alelo A fue de 1.0000 y, en el locus BM1815 la frecuencia del alelo A fue de 0.3295, del alelo B fue de 0.5909y del alelo C fu e de 0.0795. Se concluye que los alelos A, B y C de los loci DRB3.2 y BM1815 del Complejo Mayor de Histocompatibilidad BoLA Clase II están relacionados con la resistencia a B. microplus en ganado bovino y el alelo A del locus DRB31 está relacionado con la susceptibilidad.

  15. Mycobacterium sarraceniae sp. nov. and Mycobacterium helvum sp. nov., isolated from the pitcher plant Sarracenia purpurea.

    Science.gov (United States)

    Tran, Phuong M; Dahl, John L

    2016-11-01

    Several fast- to intermediate-growing, acid-fast, scotochromogenic bacteria were isolated from Sarracenia purpurea pitcher waters in Minnesota sphagnum peat bogs. Two strains (DL734T and DL739T) were among these isolates. On the basis of 16S rRNA gene sequences, the phylogenetic positions of both strains is in the genus Mycobacterium with no obvious relation to any characterized type strains of mycobacteria. Phenotypic characterization revealed that neither strain was similar to the type strains of known species of the genus Mycobacterium in the collective properties of growth, pigmentation or fatty acid composition. Strain DL734T grew at temperatures between 28 and 32 °C, was positive for 3-day arylsulfatase production, and was negative for Tween 80 hydrolysis, urease and nitrate reduction. Strain DL739T grew at temperatures between 28 and 37 °C, and was positive for Tween 80 hydrolysis, urea, nitrate reduction and 3-day arylsulfatase production. Both strains were catalase-negative while only DL739T grew with 5 % NaCl. Fatty acid methyl ester profiles were unique for each strain. DL739T showed an ability to survive at 8 °C with little to no cellular replication and is thus considered to be psychrotolerant. Therefore, strains DL734T and DL739T represent two novel species of the genus Mycobacterium with the proposed names Mycobacterium sarraceniae sp. nov. and Mycobacterium helvum sp. nov., respectively. The type strains are DL734T (=JCM 30395T=NCCB 100519T) and DL739T (=JCM 30396T=NCCB 100520T), respectively.

  16. Familial ALS

    Science.gov (United States)

    Boylan, Kevin

    2015-01-01

    Synopsis Genes linked to ALS susceptibility are being identified at an increasing rate owing to advances in molecular genetic technology. Genetic mechanisms in ALS pathogenesis appear to exert major effects in ~10% of patients, but genetic factors at some level may be important components of disease risk in most ALS patients. Identification of gene variants associated with ALS has informed concepts of the pathogenesis of ALS, aided the identification of therapeutic targets, facilitated research to develop new ALS biomarkers, and supported the establishment of clinical diagnostic tests for ALS-linked genes. Translation of this knowledge to ALS therapy development is ongoing. PMID:26515623

  17. Complejo nódulo subependimario-astrocitoma subependimario gigantocelular en niños con esclerosis tuberosa Subependymal nodules-subependymal giant cell astrocytoma complex in children with tuberous sclerosis

    Directory of Open Access Journals (Sweden)

    Lucas Bongiorni

    2009-01-01

    Full Text Available El objetivo fue describir las características clínico imagenológicas de niños con esclerosis tuberosa que presentaron el complejo Nódulo Subependimario (NS-Astrocitoma Subependimario Gigantocelular(ASGC y analizar el comportamiento evolutivo de dicho "complejo" para detectar precozmente su crecimiento y evitar las complicaciones de la hipertensión endocraneana (HTE. Evaluamos 22 pacientes con diagnóstico anátomo patológico de ASGC. El diagnóstico del tumor se realizó a una media de 10.1 años. Pudimos observar la evolución de NS a ASGC; estos NS se ubicaron adyacentes al agujero de Monro y con el tiempo tuvieron un importante crecimiento con intensa captación de contraste e hidrocefalia. La aceleración en el crecimiento de estos NS y su "transformación" en ASGC se produjo a los 10 años de edad promedio, con un diámetro medio de 9 mm. Ningún NS alejado de los forámenes de Monro evolucionó a ASGC. Quince pacientes (68% fueron operados con síntomas de hipertensión endocraneana. La edad media de la cirugía fue 10.8 años. Seis pacientes presentaron déficit visual. En estos últimos, el diámetro medio mayor del tumor fue 31.5 mm, mayor que los 18.7 mm del grupo de pacientes que no presentó secuela visual. El seguimiento clínico imagenológico periódico de toda lesión subependimaria próxima a los agujeros de Monro, permitiría en etapa presintomática anticipar un tratamiento quirúrgico, que reduciría la incidencia de HTE. Estudios prospectivos podrían determinar si el complejo NS-ASGC corresponde a una misma entidad en distinta etapa evolutiva, o son dos lesiones con diferente potencial de crecimiento.The object of this paper is to describe the imaging and clinical characteristics of subependymal nodule (SN - subependymal giant cell astrocytoma (SGCA complex in tuberous sclerosis and analyze its evolution in order to attempt early detection and the prevention of intracranial hypertension. We evaluated 22 patients with

  18. Adipocinas y síndrome metabólico: múltiples facetas de un proceso fisiopatológico complejo Adipokines and metabolic syndrome: multiple aspects of a complex pathophysiological process

    Directory of Open Access Journals (Sweden)

    Julio C Sánchez N

    2010-08-01

    Full Text Available Debido a la alta morbimortalidad de las enfermedades cardiovasculares y a su relación con trastornos de base como la obesidad y el síndrome metabólico, es crucial entender cuáles son los mecanismos y procesos que desencadenan la alteración del metabolismo y a su vez la generación de dichas enfermedades. En tal sentido, el tejido adiposo y el adipocito tienen un papel fundamental en este proceso, mediante la producción de múltiples adipocinas, algunas clásicas y otras de reciente descripción, pero que hasta ahora empieza a dilucidarse en medio del complejo panorama de interacciones fisiopatológicas conducentes al desarrollo de resistencia a la insulina y del complejo desequilibrio metabólico que conlleva un sinnúmero de complicaciones clínicas. Un grupo de estas adipocinas tiene claros efectos proinflamatorios, mientras que otras pueden clasificarse como anti-inflamatorias, las cuales contrarrestan en cierta medida y hasta cierto punto las acciones de las otras. Cuando esta homeostasis se rompe, la cascada de inflamación crónica allí originada desencadena resistencia a la insulina y se inicia el desarrollo del síndrome metabólico a partir de la obesidad, que a su vez genera alteraciones de la respuesta del adipocito a diferentes estímulos. Esto, sumado a los efectos de otros elementos, configura un complejo cuadro de factores que es necesario tener en cuenta para el abordaje correcto de la obesidad y sus patologías asociadas.Due to the high morbidity and mortality of cardiovascular diseases and their relationship with basic disorders such as obesity and metabolic syndrome, the understanding of the mechanisms and processes that trigger metabolic alterations and generate such diseases, is a crucial matter. In this regard, adipose tissue and adipocytes have a crucial role in this process through the production of multiple adipokines, some of them classical and others recently described; however, until now their role is beginning

  19. Daily fluctuations in radon concentration in a Cordoba factory complex; Fluctuaciones diurnas de la concentracion de {sup 222}Rn en el complejo fabril Cordoba

    Energy Technology Data Exchange (ETDEWEB)

    Germanier, A; Perez, R; Rubio, M [CEPROCOR, Cordoba (Argentina)

    1998-07-01

    Full text: This work shows the fluctuations of indoor radon concentration in some rooms placed inside a Cordoba Factory Complex. The measurements were performed by Victoreen Radon Monitor. The system was shaped to sample single radon concentration values after one hour integration. It utilizes a passive diffusion chamber and a silicon diffused junction detector. A portion of decay products will plate-out onto the detector and emit alpha particles into the depletion region of a diffused junction detector. The alpha energy is deposited in the detector in the form of ionization which generates a charge pulse. Only alpha pulses of an energy level similar Po-218 and Po-214 are detected. The study of the meteorological parameters shows that the daily fluctuations of the radon concentration respond to the transport and dispersion processes of radon gas through the air. Air temperature, wind's direction and speed are found to be fundamentals parameters in the observed time behavior. The meteorological data were obtained by a portable station (Davis Weathe Monitor II). The radon concentration present a maximum value (1850 Bq/m{sup 3}) at the night and a minimum value (150 Bq/m{sup 3}) at the day. (author)S. [Spanish] Texto completo: En este trabajo se estudian las fluctuaciones de la concentracion de radon 222 en aire 'indoor' en distintas habitaciones ubicadas dentro y fuera del predio del Complejo Fabril Cordoba (C.F.C.). Las determinaciones se realizaron con un detector pasivo Radon Monitor 05-418, marca Victoreen. El mismo consta de una camara de difusion electrostatica y de un detector de estado solido (Si) de juntura difundida. Este instrumento se configuro para obtener integraciones para cada una de las horas del dia. El principio de funcionamiento del detector se basa en el conteo de los decaimientos {alpha} del polonio 218 y polonio 214 atrapados dentro de la camara de difusion electrostatica. El build-up ocurrido dentro de esta camara es tenido en cuenta para

  20. Mesotherapy and cutaneous Mycobacterium fortuitum infection.

    Science.gov (United States)

    Difonzo, Elisa Margherita; Campanile, Grazia Lucia; Vanzi, Laura; Lotti, Lorena

    2009-06-01

    Cutaneous infections caused by Mycobacterium fortuitum usually are a complication of trauma or postsurgical wounds. A 41-year-old woman presented with numerous dusky red nodules, abscesses and sinuses on the right buttock and on the lateral surfaces of both thighs. The lesions developed at the injection sites of mesotherapy treatment. M. fortuitum was cultured from a biopsy specimen and purulent fluid drained from lesions. The lesions had cleared completely with ciprofloxacin 500 mg b.d. for 3 weeks, and then 250 mg b.d. for another 3 weeks. This case demonstrates the importance of suspecting mycobacterial etiology in patients with nodules and abscesses in the areas of mesotherapy treatment.

  1. Mycobacterium tuberculosis effectors interfering host apoptosis signaling.

    Science.gov (United States)

    Liu, Minqiang; Li, Wu; Xiang, Xiaohong; Xie, Jianping

    2015-07-01

    Tuberculosis remains a serious human public health concern. The coevolution between its pathogen Mycobacterium tuberculosis and human host complicated the way to prevent and cure TB. Apoptosis plays subtle role in this interaction. The pathogen endeavors to manipulate the apoptosis via diverse effectors targeting key signaling nodes. In this paper, we summarized the effectors pathogen used to subvert the apoptosis, such as LpqH, ESAT-6/CFP-10, LAMs. The interplay between different forms of cell deaths, such as apoptosis, autophagy, necrosis, is also discussed with a focus on the modes of action of effectors, and implications for better TB control.

  2. Mycobacterium chimaera left ventricular assist device infections.

    Science.gov (United States)

    Balsam, Leora B; Louie, Eddie; Hill, Fred; Levine, Jamie; Phillips, Michael S

    2017-06-01

    A global outbreak of invasive Mycobacterium chimaera infections after cardiac surgery has recently been linked to bioaerosols from contaminated heater-cooler units. The majority of cases have occurred after valvular surgery or aortic graft surgery and nearly half have resulted in death. To date, infections in patients with left ventricular assist devices (LVADs) have not been characterized in the literature. We report two cases of device-associated M. chimaera infection in patients with continuous-flow LVADs and describe challenges related to diagnosis and management in this population. © 2017 Wiley Periodicals, Inc.

  3. Skin granulomas due to Mycobacterium gordonae.

    Science.gov (United States)

    Gengoux, P; Portaels, F; Lachapelle, J M; Minnikin, D E; Tennstedt, D; Tamigneau, P

    1987-04-01

    A 38-year-old woman presented with small, ulcerated, red or bluish nodules on the right hand, clinically resembling mycobacterial granulomas; these appeared a few months after a bite by a rat, while the patient was collecting frogs in a pond in the Belgian Ardennes. The histopathologic picture was compatible with a diagnosis of mycobacterial infection and rare acid-fast bacilli could be found. Repeated bacteriologic investigations were performed and these led to the identification of a strain displaying characteristics of Mycobacterium gordonae. The skin condition responded well to rifampicin (300 mg/day) within 6 months.

  4. Formación de complejos fármaco-resina usados en preparados de liberación prolongada

    Directory of Open Access Journals (Sweden)

    Susana Alba Delgado

    1998-12-01

    Full Text Available Se estudiaron los tiempos para alcanzar el equilibrio en reacciones de intercambio iónico durante la formación de sales complejas poliestireno divinil-benceno sulfonato, con los fármacos dexanfetamina, metilhomatropina y salbutamol. Se hicieron reaccionar sales de los fármacos en solución con la resina en su forma "sodio", cuantificándose a intervalos la concentración en el sobrenadante, siguiendo el avance de la reacción y determinando el punto de equilibrio. Se propone un estadístico simple que permite seleccionar el tiempo para dar por terminada la reacción, y se encuentran valores inferiores a los informados en la literatura. Se comenta la relación entre dimensión molecular y nivel de saturación del complejo.Time intervals required for reaching the proper balance of ion exchange reactions during the formation of polystirene-dyvinil-benzene-sulphonate complex salts with drugs such as dexamphetamina, metylhomatropine and salbutamol are analyzed. Drug salt solutions were caused to react with resin in "sodium" form, then supernadant concentration was estimated at intervals following the development of the reaction and determining the point of balance. A simple statistical method which makes it possible to determine the time when the reaction shall be finished was proposed, and also values lower than those reported in literature were found. Some comment on the association between molecular dimension and the saturation level of the drug-resine complex are made

  5. Mycobacterium spp. in wild game in Slovenia.

    Science.gov (United States)

    Pate, Mateja; Zajc, Urška; Kušar, Darja; Žele, Diana; Vengušt, Gorazd; Pirš, Tina; Ocepek, Matjaž

    2016-02-01

    Wildlife species are an important reservoir of mycobacterial infections that may jeopardise efforts to control and eradicate bovine tuberculosis (bTB), caused by Mycobacterium bovis. Slovenia is officially free of bTB, but no data on the presence of mycobacteria in wild animals has been reported. In this study, samples of liver and lymph nodes were examined from 306 apparently healthy free-range wild animals of 13 species in Slovenia belonging to the families Cervidae, Suidae, Canidae, Mustelidae and Bovidae. Mycobacteria were isolated from 36/306 (11.8%) animals (red deer, roe deer, fallow deer, wild boar and jackal) and identified by PCR, commercial diagnostic kits and sequencing. Non-tuberculous mycobacteria identified in five species were Mycobacterium peregrinum, M. avium subsp. hominissuis, M. intracellulare, M. confluentis, M. fortuitum, M. terrae, M. avium subsp. avium, M. celatum, M. engbaekii, M. neoaurum, M. nonchromogenicum and M. vaccae. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Mycobacterium canettii Infection of Adipose Tissues.

    Science.gov (United States)

    Bouzid, Fériel; Brégeon, Fabienne; Poncin, Isabelle; Weber, Pascal; Drancourt, Michel; Canaan, Stéphane

    2017-01-01

    Adipose tissues were shown to host Mycobacterium tuberculosis which is persisting inside mature adipocytes. It remains unknown whether this holds true for Mycobacterium canettii , a rare representative of the M. tuberculosis complex responsible for lymphatic and pulmonary tuberculosis. Here, we infected primary murine white and brown pre-adipocytes and murine 3T3-L1 pre-adipocytes and mature adipocytes with M. canettii and M. tuberculosis as a positive control. Both mycobacteria were able to infect 18-22% of challenged primary murine pre-adipocytes; and to replicate within these cells during a 7-day experiment with the intracellular inoculums being significantly higher in brown than in white pre-adipocytes for M. canettii ( p = 0.02) and M. tuberculosis ( p = 0.03). Further in-vitro infection of 3T3-L1 mature adipocytes yielded 9% of infected cells by M. canettii and 17% of infected cells by M. tuberculosis ( p = 0.001). Interestingly, M. canettii replicated and accumulated intra-cytosolic lipid inclusions within mature adipocytes over a 12-day experiment; while M. tuberculosis stopped replicating at day 3 post-infection. These results indicate that brown pre-adipocytes could be one of the potential targets for M. tuberculosis complex mycobacteria; and illustrate differential outcome of M. tuberculosis complex mycobacteria into adipose tissues. While white adipose tissue is an unlikely sanctuary for M. canettii , it is still an open question whether M. canettii and M. tuberculosis could persist in brown adipose tissues.

  7. Dry-heat inactivation of "Mycobacterium canettii".

    Science.gov (United States)

    Aboubaker Osman, Djaltou; Garnotel, Eric; Drancourt, Michel

    2017-06-09

    "Mycobacterium canettii" is responsible for non-transmissible lymph node and pulmonary tuberculosis in persons exposed in the Horn of Africa. In the absence of direct human transmission, contaminated water and foodstuffs could be sources of contamination. We investigated the dry-heat inactivation of "M. canettii" alone and mixed into mock-infected foodstuffs by inoculating agar cylinders and milk with 10 4 colony-forming units of "M. canettii" CIPT140010059 and two "M. canettii" clinical strains with Mycobacterium tuberculosis H37Rv as a control. Exposed to 35 °C, M. tuberculosis H37Rv, "M canettii" CIPT140010059 and "M. canettii" 157 exhibited a survival rate of 108, 95 and 81%, which is significantly higher than that of "M. canettii" 173. However, all tested mycobacteria tolerated a 90-min exposure at 45 °C. In the foodstuff models set at 70 °C, no growing mycobacteria were visualized. This study supports the premise that "M. canettii" may survive up to 45 °C; and suggests that contaminated raw drinks and foodstuffs but not cooked ones may be sources of infection for populations.

  8. Images of mycobacterium for nuclear reactions

    International Nuclear Information System (INIS)

    Lima, C.T.S.; Crispim, V.R.; Silva, M.G.

    2007-01-01

    According to the World Health Organization (WHO) tuberculosis is responsible for 2.9 million deaths annually worldwide. The necessity for optimizing time to detect the tuberculosis bacillus (mycobacterium tuberculosis) in the sputum samples of affected individuals (TB patients) led to the development of a methodology based on the doping with boron of the bacillus, submission of the samples to thermal neutron beam and ionizing particles, generating nuclear reactions of the types: 10 B (n,α) 7 Li and 10 B(α, p) 13 C. Images of these bacilli are obtained by means of the nuclear tracks produced in the CR-39 detector for particles products of these nuclear reactions, α and p. When the CR-39 is submitted to a chemical attack the traces are developed and the images of the microorganisms registered in the detector can be observed with a conventional light microscope, characterizing them by morphology. The use of this methodology results in images of the mycobacterium tuberculosis becoming more defined and enlarged than those obtained by bacilloscopy, in which the sample is submitted to the method of coloration of Ziehl-Neelsen (ZN) and observed in light microscopy. (author)

  9. Mecanismos Moleculares de la Sensibilidad Aguda al Oxígeno en los Quimiorreceptores Arteriales: Función del Complejo I Mitocondrial.

    OpenAIRE

    Fernández-Agüera Rodríguez, María del Carmen

    2017-01-01

    El oxígeno (O2) es una molécula esencial para la vida de los animales debido a su metabolismo celular aeróbico, ya que la energía necesaria para realizar las funciones vitales la obtienen, principalmente, de la mitocondria mediante la fosforilación oxidativa; un proceso que requiere O2 como aceptor final de electrones. La falta de O2, aunque sólo sea de manera focalizada y transitoria, puede dañar gravemente los tejidos. A lo largo de la evol...

  10. Radiometric assessment of the sensitivity to antituberculotics of Mycobacterium avium-intracellulare and Mycobacterium xenopi

    International Nuclear Information System (INIS)

    Kubin, M.; Lindholm-Levy, P.; Heifets, L. B.

    1994-01-01

    The macrodilution radiometric method using Middlebrook's 7H12 liquid medium enriched with 14 C-palmitic acid, where the growth activity is monitored by measuring liberated 14 CO 2 , was applied to 25 strains of the Mycobacterium avium complex and to 20 strains of Mycobacterium xenopi to determine the minimal inhibitory concentrations of the following chemotherapeutical agents: ciprofloxacine, clofazimine, rifampin, cycloserine, kanamycin, etionamide, ethambutol, and amikacin. In the case of the M. avium complex, slightly or completely resistant strains were found for the majority of drugs. The sensitive strain proportion was highest with clofazimine and amikacin. The M. xenopis strains exhibited generally lower minimal inhibitory concentrations than the avian mycobacteria for all drugs except for cycloserine and ethambutol. The radiometric method using the BACTEC system was found suitable for the determination of the sensitivity of mycobacteria to chemotherapeutic agents: the results are obtained rapidly, within 8 days following inoculation, and the minimal inhibitory concentrations can be evaluated quantitatively. 1 tab., 8 refs

  11. Distinct Spatiotemporal Dynamics of Peptidoglycan Synthesis between Mycobacterium smegmatis and Mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Helene Botella

    2017-09-01

    Full Text Available Peptidoglycan (PG, a polymer cross-linked by d-amino acid-containing peptides, is an essential component of the bacterial cell wall. We found that a fluorescent d-alanine analog (FDAA incorporates chiefly at one of the two poles in Mycobacterium smegmatis but that polar dominance varies as a function of the cell cycle in Mycobacterium tuberculosis: immediately after cytokinesis, FDAAs are incorporated chiefly at one of the two poles, but just before cytokinesis, FDAAs are incorporated comparably at both. These observations suggest that mycobacterial PG-synthesizing enzymes are localized in functional compartments at the poles and septum and that the capacity for PG synthesis matures at the new pole in M. tuberculosis. Deeper knowledge of the biology of mycobacterial PG synthesis may help in discovering drugs that disable previously unappreciated steps in the process.

  12. A Case of False-Positive Mycobacterium tuberculosis Caused by Mycobacterium celatum

    Directory of Open Access Journals (Sweden)

    Edward Gildeh

    2016-01-01

    Full Text Available Mycobacterium celatum is a nontuberculous mycobacterium shown to cause symptoms similar to pulmonary M. tuberculosis. Certain strains have been shown to cross-react with the probes used to detect M. tuberculosis, making this a diagnostic challenge. We present a 56-year-old gentleman who developed signs and symptoms of lung infection with computed tomography scan of the chest showing right lung apex cavitation. Serial sputum samples were positive for acid-fast bacilli and nucleic acid amplification testing identified M. tuberculosis ribosomal RNA, resulting in treatment initiation. Further testing with high performance liquid chromatography showed a pattern consistent with M. celatum. This case illustrates the potential for M. celatum to mimic M. tuberculosis in both its clinical history and laboratory testing due to the identical oligonucleotide sequence contained in both. An increasing number of case reports suggest that early reliable differentiation could reduce unnecessary treatment and public health intervention associated with misdiagnosed tuberculosis.

  13. Interdisciplinariedad y sistemas complejos

    OpenAIRE

    García, Rolando

    2011-01-01

    Las situaciones a las cuales se suele aplicar la expresión "problemas ambientales" cubren un amplio espectro: en un extremo, aparecen problemas locales y circunscriptos, como puede ser el caso de un río contaminado por los desechos de una fábrica próxima que torna tóxica el agua que se bebe en una ciudad situada a sus orillas. En el otro, se incluyen situaciones tales como las condiciones insalubres de vida en grandes centros urbanos, o el deterioro del medio físico y de las condiciones de vi...

  14. [Cutaneous infection by Mycobacterium fortuitum]  Infeccion cutanea por Mycobacterium fortuitum

    Directory of Open Access Journals (Sweden)

    Verónica Rotela

    2017-10-01

    Full Text Available Mycobacteria are aerobic, non-spore forming, gram positive, acid-fast bacilli, which affect skin, subcutaneous tissue, and other organs and systems. Mycobacterium fortuitum produces cellulitis, abscesses, papules-pustules, nodules and ulcers with serosanguinolent, purulent material, and subcutaneous necrosis. A 61-year-old woman, presents a case of two months of evolution that begins with reddish grain from an insect sting. After immersion in the Mexican Sea, it worsens, increases in quantity, is blistered and has brownish secretion; Physical examination shows erythematous plaque, with punctate orifices with hematic and meliceric crusts; Pustules and satellite papules, on the anterior aspect of the right leg. Histopathology: Suppurative dermal granulomas, centered by acute leukocyte infiltrate, with liquefactive tissue necrosis, surrounded by chronic inflammation with macrophages, plasma cells, lymphocytes, multinucleated giant cells. The first skin culture returns negative; in the second skin culture, fast-growing, non-pigmented atypical mycobacteria. Molecular detection is performed by Polymerase Chain Reaction: Mycobacterium fortuitum. Treatment with Ciprofloxacin 500 mg every 12 hours, with resolution of the table to the eighth month. A case of cutaneous infection by Mycobacterium fortuitum, related to the immersion in the sea and corals, whose diagnostic process has been difficult and was achieved by techniques of advanced molecular biology.

  15. Chronic breast abscess due to Mycobacterium fortuitum: a case report

    Directory of Open Access Journals (Sweden)

    MacNeill Fiona A

    2011-05-01

    Full Text Available Abstract Introduction Mycobacterium fortuitum is a rapidly growing group of nontuberculous mycobacteria more common in patients with genetic or acquired causes of immune deficiency. There have been few published reports of Mycobacterium fortuitum associated with breast infections mainly associated with breast implant and reconstructive surgery. Case presentation We report a case of a 51-year-old Caucasian woman who presented to our one-stop breast clinic with a two-week history of left breast swelling and tenderness. Following triple assessment and subsequent incision and drainage of a breast abscess, the patient was diagnosed with Mycobacterium fortuitum and treated with antibiotic therapy and surgical debridement. Conclusion This is a rare case of a spontaneous breast abscess secondary to Mycobacterium fortuitum infection. Recommended treatment is long-term antibacterial therapy and surgical debridement for extensive infection or when implants are involved.

  16. Buoyant density of Mycobacterium tuberculosis: implications for sputum processing

    NARCIS (Netherlands)

    den Hertog, A. L.; Klatser, P. R.; Anthony, R. M.

    2009-01-01

    A tuberculosis (TB) research laboratory in the Netherlands. The concentration of Mycobacterium tuberculosis cells from sputum is almost universally performed by centrifugation after chemical liquefaction. These methods are thus dependent on the effective sedimentation of mycobacterial cells, and the

  17. Variable host-pathogen compatibility in Mycobacterium tuberculosis.

    NARCIS (Netherlands)

    Gagneux, Sebastien; DeRiemer, Kathryn; Van, Tran; Kato-Maeda, Midori; Jong, Bouke C de; Narayanan, Sujatha; Nicol, Mark; Niemann, Stefan; Kremer, Kristin; Gutierrez, M Cristina; Hilty, Markus; Hopewell, Philip C; Small, Peter M

    2006-01-01

    Mycobacterium tuberculosis remains a major cause of morbidity and mortality worldwide. Studies have reported human pathogens to have geographically structured population genetics, some of which have been linked to ancient human migrations. However, no study has addressed the potential evolutionary

  18. EVIDENCE FOR THE MACROPHAGE INDUCING GENE IN MYCOBACTERIUM INTRACELLULARE

    Science.gov (United States)

    Background: The Mycobacterium avium Complex (MAC) includes the species M. avium (MA), M. intracellulare (MI), and possibly others. Organisms belonging to the MAC are phylogenetically closely related, opportunistic pathogens. The macrophage inducing gene (mig) is the only well-des...

  19. A robust SNP barcode for typing Mycobacterium tuberculosis complex strains

    KAUST Repository

    Coll, Francesc; McNerney, Ruth; Guerra-Assunç ã o, José Afonso; Glynn, Judith R.; Perdigã o, Joã o; Viveiros, Miguel; Portugal, Isabel; Pain, Arnab; Martin, Nigel; Clark, Taane G.

    2014-01-01

    Strain-specific genomic diversity in the Mycobacterium tuberculosis complex (MTBC) is an important factor in pathogenesis that may affect virulence, transmissibility, host response and emergence of drug resistance. Several systems have been proposed

  20. Drug susceptibility testing of Mycobacterium tuberculosis to fluoroquinolones

    DEFF Research Database (Denmark)

    Johansen, I S; Larsen, A R; Sandven, P

    2003-01-01

    In the first attempt to establish a quality assurance programme for susceptibility testing of Mycobacterium tuberculosis to fluoroquinolones, 20 strains with different fluoroquinolone susceptibility patterns were distributed by the Supranational Reference Laboratory in Stockholm to the other...

  1. Bloodstream Infections with Mycobacterium tuberculosis among HIV patients

    Centers for Disease Control (CDC) Podcasts

    This podcast looks at bloodstream infections with Mycobacterium tuberculosis and other pathogens among outpatients infected with HIV in Southeast Asia. CDC health scientist Kimberly McCarthy discusses the study and why bloodstream infections occur in HIV-infected populations.

  2. Complete Genome Sequence of Mycobacterium xenopi Type Strain RIVM700367

    KAUST Repository

    Abdallah, A. M.; Rashid, M.; Adroub, S. A.; Elabdalaoui, H.; Ali, Shahjahan; van Soolingen, D.; Bitter, W.; Pain, Arnab

    2012-01-01

    Mycobacterium xenopi is a slow-growing, thermophilic, water-related Mycobacterium species. Like other nontuberculous mycobacteria, M. xenopi more commonly infects humans with altered immune function, such as chronic obstructive pulmonary disease patients. It is considered clinically relevant in a significant proportion of the patients from whom it is isolated. We report here the whole genome sequence of M. xenopi type strain RIVM700367.

  3. Complete Genome Sequence of Mycobacterium xenopi Type Strain RIVM700367

    KAUST Repository

    Abdallah, A. M.

    2012-05-24

    Mycobacterium xenopi is a slow-growing, thermophilic, water-related Mycobacterium species. Like other nontuberculous mycobacteria, M. xenopi more commonly infects humans with altered immune function, such as chronic obstructive pulmonary disease patients. It is considered clinically relevant in a significant proportion of the patients from whom it is isolated. We report here the whole genome sequence of M. xenopi type strain RIVM700367.

  4. Mycobacterium chelonae empyema with bronchopleural fistula in an immunocompetent patient

    International Nuclear Information System (INIS)

    Wali, Siraj

    2009-01-01

    Mycobacterium Calhoun is one of the rapidly growing mycobacteria that rarely cause lung disease. M chelonae more commonly causes skin and soft tissue infections primarily in immunosuppressed individuals. Thoracic empyema caused by rapidly growing mycobacteria and complicated with bronchopleural fistula is rarely reported, especially in immunocompetent patients. In this article we report the first immunocompetent Arabian patient presented with M chelonae- related empyema with bronchopleural fistula which mimics, clinically and radiologically, empyema caused by Mycobacterium tuberculosis. (author)

  5. Mycobacterium Diversity and Pyrene Mineralization in Petroleum-Contaminated Soils

    OpenAIRE

    Cheung, Pui-Yi; Kinkle, Brian K.

    2001-01-01

    Degradative strains of fast-growing Mycobacterium spp. are commonly isolated from polycyclic aromatic hydrocarbon (PAH)-contaminated soils. Little is known, however, about the ecology and diversity of indigenous populations of these fast-growing mycobacteria in contaminated environments. In the present study 16S rRNA genes were PCR amplified using Mycobacterium-specific primers and separated by temperature gradient gel electrophoresis (TGGE), and prominent bands were sequenced to compare the ...

  6. Mycobacterium arupense, Mycobacterium heraklionense, and a Newly Proposed Species, “Mycobacterium virginiense” sp. nov., but Not Mycobacterium nonchromogenicum, as Species of the Mycobacterium terrae Complex Causing Tenosynovitis and Osteomyelitis

    Science.gov (United States)

    Vasireddy, Sruthi; Brown-Elliott, Barbara A.; Wengenack, Nancy L.; Eke, Uzoamaka A.; Benwill, Jeana L.; Turenne, Christine; Wallace, Richard J.

    2016-01-01

    Mycobacterium terrae complex has been recognized as a cause of tenosynovitis, with M. terrae and Mycobacterium nonchromogenicum reported as the primary etiologic pathogens. The molecular taxonomy of the M. terrae complex causing tenosynovitis has not been established despite approximately 50 previously reported cases. We evaluated 26 isolates of the M. terrae complex associated with tenosynovitis or osteomyelitis recovered between 1984 and 2014 from 13 states, including 5 isolates reported in 1991 as M. nonchromogenicum by nonmolecular methods. The isolates belonged to three validated species, one new proposed species, and two novel related strains. The majority of isolates (20/26, or 77%) belonged to two recently described species: Mycobacterium arupense (10 isolates, or 38%) and Mycobacterium heraklionense (10 isolates, or 38%). Three isolates (12%) had 100% sequence identity to each other by 16S rRNA and 99.3 to 100% identity by rpoB gene region V sequencing and represent a previously undescribed species within the M. terrae complex. There were no isolates of M. terrae or M. nonchromogenicum, including among the five isolates reported in 1991. The 26 isolates were susceptible to clarithromycin (100%), rifabutin (100%), ethambutol (92%), and sulfamethoxazole or trimethoprim-sulfamethoxazole (70%). The current study suggests that M. arupense, M. heraklionense, and a newly proposed species (“M. virginiense” sp. nov.; proposed type strain MO-233 [DSM 100883, CIP 110918]) within the M. terrae complex are the major causes of tenosynovitis and osteomyelitis in the United States, with little change over 20 years. Species identification within this complex requires sequencing methods. PMID:26962085

  7. Astrocitoma subependimario de células gigantes asociado a complejo de esclerosis tuberosa: recomendaciones para el diagnóstico oportuno y tratamiento.

    OpenAIRE

    Matilde Ruiz; Sandra Sánchez; Alejandro Rea; Roberto Sanromán; Joao García; Antonio Bravo; Infante Cantú; Hugo Ceja; Ariadna González; María del Mar Sáez

    2015-01-01

    RESUMEN El complejo de esclerosis tuberosa es una enfermedad genética poco frecuente, autosómica dominante con fenotipo y expresión clínica muy variables. Se caracteriza por alteraciones en la migración, diferenciación y proliferación celulares con formación de múltiples tumores benignos llamados hamartomas, las cuales afectan principalmente piel, encéfalo, riñón, ojo, corazón y pulmón. Los astrocitomas subependimarios de células gigantes son tumores benignos de crecimien...

  8. Gestión organizacional en entornos complejos por parte de las Mipymes del sector servicios de la ciudad de Sincelejo

    OpenAIRE

    Jorge Luis Del Rio Vásquez

    2017-01-01

    Con el propósito de determinar los modelos de gestión organizacional en entornos complejos que practican las MIPYMES del sector servicios en la ciudad de Sincelejo - Sucre - Colombia, se adelanta la presente investigación. Para ello es importante tener en cuenta su gestión actual, toda vez que estas representan en su mayoría el tejido empresarial de las economías locales y nacionales. El presente artículo pretende un análisis, a la luz de la teoría y antecedentes empíricos, que demuestre l...

  9. Estudio de reacciones de oxidación catalizadas por complejos biomiméticos de Fe (II), Fe (III) y Mn (III)

    OpenAIRE

    Clemente Tejeda, David

    2015-01-01

    [ES]Se ha desarrollado una metodología económicamente asequible y medioambientalmente sostenible para la oxidación catalítica de sustratos orgánicos con interés preparativo, utilizando para ello sistemas biomiméticos de Fe(II), Fe(III) y Mn(III). En primer lugar se ha llevado a cabo la síntesis de los complejos metálicos catalizadores, optimizando los procedimientos descritos en la bibliografía precedente. Asimismo se ha llevado a cabo un análisis exhaustivo de todas las variables implicadas ...

  10. Exodoncia de diente supernumerario, canino en transposición y enucleación de odontoma complejo: Reporte de Caso

    OpenAIRE

    Perea Paz, Miguel; Aliaga Del Castillo, Rosalinda; Aliaga Del Castillo, Arón

    2014-01-01

    Las anomalías dentarias más frecuentes e importantes que afectan a los niños son la presencia de piezas supernumerarias, piezas retenidas y odontomas. Algunos dientes retenidos se pueden encontrar en  transposición. Reportamos el caso de una paciente de sexo femenino de 11 años de edad con presencia de diente supernumerario, canino en transposición y odontoma complejo situados en la hemiarcada derecha del maxilar superior. El motivo de consulta fue por la presencia de dientes anteriores sobre...

  11. Sistemas de ayuda a la decisión clínica en enfermedades de diagnóstico complejo

    OpenAIRE

    Monsalve Torra, Ana

    2017-01-01

    El trabajo de investigación presentado en esta tesis doctoral aborda el uso de algoritmos de inteligencia artificial y su aplicación en los sistemas de ayuda para la toma de decisiones en enfermedades de diagnóstico complejo. La dificultad en la gestión clínica de dichas enfermedades requiere un trabajo en conjunto (área de salud e ingeniería informática) y un intercambio de conocimientos, experiencias e información que permita obtener mejores resultados en el proceso de atención de los enfer...

  12. El impacto del cambio climático en los glaciares del complejo volcánico Nevado Coropuna, (Cordillera Occidental de los Andes Centrales)

    OpenAIRE

    Ubeda Palenque, José

    2010-01-01

    El objetivo de la investigación ha sido evaluar el impacto del cambio climático en los glaciares del Nevado Coropuna, que en la actualidad recubren el área de cumbres de ese complejo de edificios volcánicos alcanzando una superficie de casi medio centenar de km2. El interés del trabajo de investigación se debe a tres razones principales: 1) De esas masas de hielo depende el abastecimiento de varias decenas de miles de personas; 2) El escenario de riesgo que se deduce de la localización vulner...

  13. Mycobacterium Tuberculosis Pyomyositis in an Infant

    Science.gov (United States)

    Malik, ZA; Shehab, M

    2013-01-01

    Mycobacterium tuberculosis is endemic to many parts of the world. It may have variable clinical presentations, especially in the pediatric age group. Presented here is the case of a 9-month old infant who was referred for infectious disease opinion when his thigh induration failed to improve after surgical drainage and a course of oral antibiotic therapy. Mycobacterial PCR on the operative sample fluid was found to be positive; and mycobacterial culture grew M. tuberculosis. He received 9 months of treatment with anti-TB medications, with excellent results and complete recovery. This is the first report of TB pyomyositis in an infant; and highlights the need to have a high index of suspicion for unusual organisms when conventional therapy fails to demonstrate expected results. PMID:23919207

  14. Virulence factors of the Mycobacterium tuberculosis complex

    Science.gov (United States)

    Forrellad, Marina A.; Klepp, Laura I.; Gioffré, Andrea; Sabio y García, Julia; Morbidoni, Hector R.; Santangelo, María de la Paz; Cataldi, Angel A.; Bigi, Fabiana

    2013-01-01

    The Mycobacterium tuberculosis complex (MTBC) consists of closely related species that cause tuberculosis in both humans and animals. This illness, still today, remains to be one of the leading causes of morbidity and mortality throughout the world. The mycobacteria enter the host by air, and, once in the lungs, are phagocytated by macrophages. This may lead to the rapid elimination of the bacillus or to the triggering of an active tuberculosis infection. A large number of different virulence factors have evolved in MTBC members as a response to the host immune reaction. The aim of this review is to describe the bacterial genes/proteins that are essential for the virulence of MTBC species, and that have been demonstrated in an in vivo model of infection. Knowledge of MTBC virulence factors is essential for the development of new vaccines and drugs to help manage the disease toward an increasingly more tuberculosis-free world. PMID:23076359

  15. MYCOBACTERIUM AVIUM SUSP. PARATUBERCULOSIS IN DAIRY PRODUCTION

    Directory of Open Access Journals (Sweden)

    G. Marchetti

    2012-08-01

    Full Text Available Mycobacterium avium subsp. paratuberculosis (MAP is the etiologic agent of paratuberculosis. The disease affects cows and other ruminants and causes high economic losses, mainly for dairy production. MAP may also have a role in the development of Crohn’s disease in humans. Infected animals shed viable MAP with milk and faeces and humans may assume MAP via the consumption of contaminated milk and dairy products. Current methods of milk pasteurization are not sufficient to kill all MAP cells present in milk and MAP has been found in raw or pasteurized milk and isolated from cheese. The aim of this paper is to review the current knowledge about MAP in dairy production. We analyzed studies on milk contamination, effect of pasteurization and methods for identification of MAP that can be applied to dairy products.

  16. Advances in Proteomics of Mycobacterium leprae.

    Science.gov (United States)

    Parkash, O; Singh, B P

    2012-04-01

    Although Mycobacterium leprae was the first bacterial pathogen identified causing human disease, it remains one of the few that is non-cultivable. Understanding the biology of M. leprae is one of the primary challenges in current leprosy research. Genomics has been extremely valuable, nonetheless, functional proteins are ultimately responsible for controlling most aspects of cellular functions, which in turn could facilitate parasitizing the host. Furthermore, bacterial proteins provide targets for most of the vaccines and immunodiagnostic tools. Better understanding of the proteomics of M. leprae could also help in developing new drugs against M. leprae. During the past nearly 15 years, there have been several developments towards the identification of M. leprae proteins employing contemporary proteomics tools. In this review, we discuss the knowledge gained on the biology and pathogenesis of M. leprae from current proteomic studies. © 2012 The Authors. Scandinavian Journal of Immunology © 2012 Blackwell Publishing Ltd.

  17. Mycobacterium leprae: genes, pseudogenes and genetic diversity

    Science.gov (United States)

    Singh, Pushpendra; Cole, Stewart T

    2011-01-01

    Leprosy, which has afflicted human populations for millenia, results from infection with Mycobacterium leprae, an unculturable pathogen with an exceptionally long generation time. Considerable insight into the biology and drug resistance of the leprosy bacillus has been obtained from genomics. M. leprae has undergone reductive evolution and pseudogenes now occupy half of its genome. Comparative genomics of four different strains revealed remarkable conservation of the genome (99.995% identity) yet uncovered 215 polymorphic sites, mainly single nucleotide polymorphisms, and a handful of new pseudogenes. Mapping these polymorphisms in a large panel of strains defined 16 single nucleotide polymorphism-subtypes that showed strong geographical associations and helped retrace the evolution of M. leprae. PMID:21162636

  18. Consequences of genomic diversity in Mycobacterium tuberculosis

    Science.gov (United States)

    Coscolla, Mireia; Gagneux, Sebastien

    2014-01-01

    The causative agent of human tuberculosis, Mycobacterium tuberculosis complex (MTBC), comprises seven phylogenetically distinct lineages associated with different geographical regions. Here we review the latest findings on the nature and amount of genomic diversity within and between MTBC lineages. We then review recent evidence for the effect of this genomic diversity on mycobacterial phenotypes measured experimentally and in clinical settings. We conclude that overall, the most geographically widespread Lineage 2 (includes Beijing) and Lineage 4 (also known as Euro-American) are more virulent than other lineages that are more geographically restricted. This increased virulence is associated with delayed or reduced pro-inflammatory host immune responses, greater severity of disease, and enhanced transmission. Future work should focus on the interaction between MTBC and human genetic diversity, as well as on the environmental factors that modulate these interactions. PMID:25453224

  19. Associations between Mycobacterium tuberculosis Strains and Phenotypes

    Science.gov (United States)

    Brown, Timothy; Nikolayevskyy, Vladyslav; Velji, Preya

    2010-01-01

    To inform development of tuberculosis (TB) control strategies, we characterized a total of 2,261 Mycobacterium tuberculosis complex isolates by using multiple phenotypic and molecular markers, including polymorphisms in repetitive sequences (spoligotyping and variable-number tandem repeats [VNTRs]) and large sequence and single-nucleotide polymorphisms. The Beijing family was strongly associated with multidrug resistance (p = 0.0001), and VNTR allelic variants showed strong associations with spoligotyping families: >5 copies at exact tandem repeat (ETR) A, >2 at mycobacterial interspersed repetitive unit 24, and >3 at ETR-B associated with the East African–Indian and M. bovis strains. All M. tuberculosis isolates were differentiated into 4 major lineages, and a maximum parsimony tree was constructed suggesting a more complex phylogeny for M. africanum. These findings can be used as a model of pathogen global diversity. PMID:20113558

  20. Mycobacterium abscessus Complex Infections in Humans.

    Science.gov (United States)

    Lee, Meng-Rui; Sheng, Wang-Huei; Hung, Chien-Ching; Yu, Chong-Jen; Lee, Li-Na; Hsueh, Po-Ren

    2015-09-01

    Mycobacterium abscessus complex comprises a group of rapidly growing, multidrug-resistant, nontuberculous mycobacteria that are responsible for a wide spectrum of skin and soft tissue diseases, central nervous system infections, bacteremia, and ocular and other infections. M. abscessus complex is differentiated into 3 subspecies: M. abscessus subsp. abscessus, M. abscessus subsp. massiliense, and M. abscessus subsp. bolletii. The 2 major subspecies, M. abscessus subsp. abscessus and M. abscessus subsp. massiliense, have different erm(41) gene patterns. This gene provides intrinsic resistance to macrolides, so the different patterns lead to different treatment outcomes. M. abscessus complex outbreaks associated with cosmetic procedures and nosocomial transmissions are not uncommon. Clarithromycin, amikacin, and cefoxitin are the current antimicrobial drugs of choice for treatment. However, new treatment regimens are urgently needed, as are rapid and inexpensive identification methods and measures to contain nosocomial transmission and outbreaks.

  1. Autoradiographic and metabolic studies of Mycobacterium leprae

    International Nuclear Information System (INIS)

    Khanolkar, S.R.; Ambrose, E.J.; Chulawala, R.G.; Bapat, C.V.

    1978-01-01

    Highly purified suspensions of Mycobacterium leprae show a progressive increase in incorporation of [ 3 H]thymidine and [ 3 H]DOPA in short-term cultures as shown by scintillation counting. The intact bacilli are known to have a high permeability barrier. The experiments described suggest that [ 3 H]DOPA becomes trapped within this barrier and oxidized inside the bacilli. Tests by pre-treatment with diethyl dithiocarbamate (DDC inhibitor of DOPA), cold DOPA or hyaluronidase distinguish the uptake of [ 3 H]DOPA by bacilli from the effects of connective tissue contamination. Similar increases in labelling of bacilli by scintillation counting of cultures, have been observed by autoradiography of the organisms. The scintillation method shows promise for rapidly identifying drug resistance in lepromatous patients relapsing while on treatment with dapsone (DDS) rifampicin, clofazimine or other anti-leprosy drugs. (author)

  2. Phenotypic and genomic comparison of Mycobacterium aurum and surrogate model species to Mycobacterium tuberculosis: implications for drug discovery.

    Science.gov (United States)

    Namouchi, Amine; Cimino, Mena; Favre-Rochex, Sandrine; Charles, Patricia; Gicquel, Brigitte

    2017-07-13

    Tuberculosis (TB) is caused by Mycobacterium tuberculosis and represents one of the major challenges facing drug discovery initiatives worldwide. The considerable rise in bacterial drug resistance in recent years has led to the need of new drugs and drug regimens. Model systems are regularly used to speed-up the drug discovery process and circumvent biosafety issues associated with manipulating M. tuberculosis. These include the use of strains such as Mycobacterium smegmatis and Mycobacterium marinum that can be handled in biosafety level 2 facilities, making high-throughput screening feasible. However, each of these model species have their own limitations. We report and describe the first complete genome sequence of Mycobacterium aurum ATCC23366, an environmental mycobacterium that can also grow in the gut of humans and animals as part of the microbiota. This species shows a comparable resistance profile to that of M. tuberculosis for several anti-TB drugs. The aims of this study were to (i) determine the drug resistance profile of a recently proposed model species, Mycobacterium aurum, strain ATCC23366, for anti-TB drug discovery as well as Mycobacterium smegmatis and Mycobacterium marinum (ii) sequence and annotate the complete genome sequence of this species obtained using Pacific Bioscience technology (iii) perform comparative genomics analyses of the various surrogate strains with M. tuberculosis (iv) discuss how the choice of the surrogate model used for drug screening can affect the drug discovery process. We describe the complete genome sequence of M. aurum, a surrogate model for anti-tuberculosis drug discovery. Most of the genes already reported to be associated with drug resistance are shared between all the surrogate strains and M. tuberculosis. We consider that M. aurum might be used in high-throughput screening for tuberculosis drug discovery. We also highly recommend the use of different model species during the drug discovery screening process.

  3. Comparison of the UDP-N-Acetylmuramate:l-Alanine Ligase Enzymes from Mycobacterium tuberculosis and Mycobacterium leprae

    Science.gov (United States)

    Mahapatra, Sebabrata; Crick, Dean C.; Brennan, Patrick J.

    2000-01-01

    In the peptidoglycan of Mycobacterium leprae, l-alanine of the side chain is replaced by glycine. When expressed in Escherichia coli, MurC (UDP-N-acetyl-muramate:l-alanine ligase) of M. leprae showed Km and Vmax for l-alanine and glycine similar to those of Mycobacterium tuberculosis MurC, suggesting that another explanation should be sought for the presence of glycine. PMID:11073931

  4. Comparison of the UDP-N-Acetylmuramate:l-Alanine Ligase Enzymes from Mycobacterium tuberculosis and Mycobacterium leprae

    OpenAIRE

    Mahapatra, Sebabrata; Crick, Dean C.; Brennan, Patrick J.

    2000-01-01

    In the peptidoglycan of Mycobacterium leprae, l-alanine of the side chain is replaced by glycine. When expressed in Escherichia coli, MurC (UDP-N-acetyl-muramate:l-alanine ligase) of M. leprae showed Km and Vmax for l-alanine and glycine similar to those of Mycobacterium tuberculosis MurC, suggesting that another explanation should be sought for the presence of glycine.

  5. Fatty Acyl Chains of Mycobacterium marinum Lipooligosaccharides

    Science.gov (United States)

    Rombouts, Yoann; Alibaud, Laeticia; Carrère-Kremer, Séverine; Maes, Emmanuel; Tokarski, Caroline; Elass, Elisabeth; Kremer, Laurent; Guérardel, Yann

    2011-01-01

    We have recently established the fine structure of the glycan backbone of lipooligosaccharides (LOS-I to LOS-IV) isolated from Mycobacterium marinum, a close relative of Mycobacterium tuberculosis. These studies culminated with the description of an unusual terminal N-acylated monosaccharide that confers important biological functions to LOS-IV, such as macrophage activation, that may be relevant to granuloma formation. It was, however, also suggested that the lipid moiety was required for LOSs to exert their immunomodulatory activity. Herein, using highly purified LOSs from M. marinum, we have determined through a combination of mass spectrometric and NMR techniques, the structure and localization of the fatty acids composing the lipid moiety. The occurrence of two distinct polymethyl-branched fatty acids presenting specific localizations is consistent with the presence of two highly related polyketide synthases (Pks5 and Pks5.1) in M. marinum and presumably involved in the synthesis of these fatty acyl chains. In addition, a bioinformatic search permitted us to identify a set of enzymes potentially involved in the biosynthesis or transfer of these lipids to the LOS trehalose unit. These include MMAR_2343, a member of the Pap (polyketide-associated protein) family, that acylates trehalose-based glycolipids in M. marinum. The participation of MMAR_2343 to LOS assembly was demonstrated using a M. marinum mutant carrying a transposon insertion in the MMAR_2343 gene. Disruption of MMAR_2343 resulted in a severe LOS breakdown, indicating that MMAR_2343, hereafter designated PapA4, fulfills the requirements for LOS acylation and assembly. PMID:21803773

  6. Multinucleated giant cell cytokine expression in pulmonary granulomas of cattle experimentally infected with Mycobacterium bovis

    Science.gov (United States)

    Pathogenic mycobacteria of the Mycobacterium tuberculosis complex such as Mycobacterium bovis, induce a characteristic lesion known as a granulomas. Granulomas represent a specific host response to chronic antigenic stimuli, such as foreign bodies, certain bacterial components, or persistent pathoge...

  7. Pott's disease: a case of Mycobacterium xenopi infection of the spine.

    Science.gov (United States)

    Alfreijat, Majd; Ononiwu, Chiagozie; Sexton, Carlton

    2012-01-01

    Pott's disease is an infection of the spine with Mycobacterium tuberculosis that causes destruction of the spine elements resulting in progressive kyphosis. We are describing a rare case of Pott's disease where Mycobacterium xenopi was the inculpated organism.

  8. La argumentación científica escolar: contribución a la comprensión de un modelo complejo de salud y enfermedad

    Directory of Open Access Journals (Sweden)

    Andrea Fernanda Revel Chion

    2014-12-01

    Full Text Available Este trabajo aborda la relación existente entre el aprendizaje de la argumentación científica escolar y la adquisición de un modelo complejo en torno a la salud y la enfermedad, más específicamente para la explicación de la emergencia o reemergencia de las enfermedades. Se asume que la argumentación científica escolar es un procedimiento cognitivo lingüístico que da lugar a la producción de un texto que explica, en el que pueden reconocerse cuatro componentes: pragmática, retórica, teórica y lógica. Se realizó un análisis histórico-epistemológico del concepto de salud y se tomó posición por un modelo complejo, que hemos denominado multicausal y multirreferencial. Se diseñó una unidad didáctica para la enseñanza de ambos contenidos con énfasis en procesos metacognitivos y autorregulatorios puestos en marcha pr medio de bases de orientación, e instancias de autoevaluación, coevaluación y heteroevaluación. El estudio realizado se alinea en un modelo de explicación ilocutivo.

  9. Complejidad, interdisciplina y política en la teoría de los sistemas complejos, de Rolando García

    Directory of Open Access Journals (Sweden)

    Leonardo G. Rodríguez Zoya

    2017-07-01

    Full Text Available Con su teoría de los Sistemas complejos, Rolando García establece un enfoque teórico-metodológico para la investigación interdisciplinaria de sistemas complejos, fundamentado en la epistemología constructivista de Jean Piaget. El objetivo de este artículo es analizar tres conceptos que subyacen en esta teoría: complejidad, interdisciplina y política. La estrategia argumental está organizada en dos etapas: en la primera se examina la relación entre complejidad e interdisciplina, y las tres concepciones de complejidad que este vínculo arroja (como concepto metodológico, como concepto organizacional y como concepto evolutivo. En la segunda etapa argumental se explora, particularmente, la dimensión política; también, las finalidades prácticas del enfoque planteado por García y las implicaciones del concepto de marco epistémico.

  10. Expresión de las moléculas del complejo mayor de histocompatibilidad clases I y II en pacientes con tuberculosis: efecto de la infección i n vitro con Mycobacterium tuberculosis H37Rv

    Directory of Open Access Journals (Sweden)

    Luis Fernando Barrera

    2001-04-01

    Full Text Available

    Es ampliamente conocido que pacientes con tuberculosis (TB
    activa pueden presentar alteraciones en la respuesta inmunológica, incluyendo defectos en la presentación antigénica. Dado el papel crítico del IFNγ y de las moléculas clase II en la inmunidad antimicrobiana, una estrategia desarrollada por microorganismos intracelulares, incluyendo virus, protozoos y bacterias, para evadir la respuesta inmune del hospedero, es la inhibición de la expresión de las moléculas del CMH. La mayor parte de las evidencias obtenidas hasta la fecha indican que estas infecciones intracelulares conducen a diferentes alteraciones en la vía de transducción de señales mediada por Jak1,2-Stat1, las
    cuales se han asociado con una disminución en la expresión superficial del CMH.
    Evidencias obtenidas en nuestro laboratorio, utilizando la línea
    celular de macrófagos murinos B10R infectada con Mtb, sugiere que esta infección altera la vía de transduccíon de señales mediada por Jak- Stat, la cual a su vez conduce a la disminución en la expresión de CIITA, un coactivador esencial para la expresión del CMH. De otro lado, se ha observado que la presencia de TGFβ e IL-10 también puede conducir a una disminución en la expresión del CMH II, a través de mecanismos
    dependientes e independientes de la vía JAK-Stat. Dado que una fracción apreciable de pacientes con TB muestran niveles elevados de estas citoquinas, ellas pudieran participar en la disminución de la expresión de las moléculas del CMH observada en algunos pacientes.

     

  11. Complete Genome Sequence of the Frog Pathogen Mycobacterium ulcerans Ecovar Liflandii

    NARCIS (Netherlands)

    Tobias, Nicholas J.; Doig, Kenneth D.; Medema, Marnix H.; Chen, Honglei; Haring, Volker; Moore, Robert; Seemann, Torsten; Stinear, Timothy P.

    In 2004, a previously undiscovered mycobacterium resembling Mycobacterium ulcerans (the agent of Buruli ulcer) was reported in an outbreak of a lethal mycobacteriosis in a laboratory colony of the African clawed frog Xenopus tropicalis. This mycobacterium makes mycolactone and is one of several

  12. L'Hemisferic: Una ventana al mundo

    Directory of Open Access Journals (Sweden)

    Calatrava Valls, Santiago

    2000-12-01

    Full Text Available The "Hemisféric-Planetario" building is located in the center line East-West of the "Ciudad de las Artes y las Ciencias" complex, flanked by two rectangular lakes, in the north and in the south, respectively. In the East side we can find a promenade between the Museum and its south lake. Nevertheless its West side is virtual. and located in the area of the "Hermanos Marista 's" viaducto The building is visited, daily, by about 3.000 people, distributed between its 8 duties. The showing room holds 321 seats, four of those for wheel-chairs. The building has a possible room of 999 people (at the same time.El edificio Hemisféric-Planetarío está situado sobre el eje Este-Oeste del complejo de la Ciudad de las Artes y las Ciencias; está flanqueado por dos estanques rectangulares, al Norte y al Sur, respectivamente. Por el Este, un paseo limita la parcela con el Museo y su estanque Sur. El limite Oeste, sin embargo. es virtual y se encuentra en la proyección del viaducto de Hermanos Maristas. Este edificio es visitado diariamente por unos 3.000 personas, distribuyéndose en 8 funciones. La sala de proyección dispone de 321 butacas, con posibilidad de alojar 4 sillas de ruedas. La capacidad de acogida del edificio (al mismo tiempo es de 999 personas.

  13. Two novel species of rapidly growing mycobacteria: Mycobacterium lehmannii sp. nov. and Mycobacterium neumannii sp. nov.

    Science.gov (United States)

    Nouioui, Imen; Sangal, Vartul; Carro, Lorena; Teramoto, Kanae; Jando, Marlen; Montero-Calasanz, Maria Del Carmen; Igual, José Mariano; Sutcliffe, Iain; Goodfellow, Michael; Klenk, Hans-Peter

    2017-12-01

    Two rapidly growing mycobacteria with identical 16S rRNA gene sequences were the subject of a polyphasic taxonomic study. The strains formed a well-supported subclade in the mycobacterial 16S rRNA gene tree and were most closely associated with the type strain of Mycobacterium novocastrense. Single and multilocus sequence analyses based on hsp65, rpoB and 16S rRNA gene sequences showed that strains SN 1900 T and SN 1904 T are phylogenetically distinct but share several chemotaxonomic and phenotypic features that are are consistent with their classification in the genus Mycobacterium. The two strains were distinguished by their different fatty acid and mycolic acid profiles, and by a combination of phenotypic features. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values for strains SN 1900 T and SN 1904 T were 61.0 % and 94.7 %, respectively; in turn, the corresponding dDDH and ANI values with M. novocastrense DSM 44203 T were 41.4 % and 42.8 % and 89.3 % and 89.5 %, respectively. These results show that strains SN1900 T and SN 1904 T form new centres of taxonomic variation within the genus Mycobacterium. Consequently, strains SN 1900 T (40 T =CECT 8763 T =DSM 43219 T ) and SN 1904 T (2409 T =CECT 8766 T =DSM 43532 T ) are considered to represent novel species, for which the names Mycobacteriumlehmannii sp. nov. and Mycobacteriumneumannii sp. nov. are proposed. A strain designated as 'Mycobacteriumacapulsensis' was shown to be a bona fide member of the putative novel species, M. lehmannii.

  14. Propiedades insecticidas de la ceniza del complejo volcánico Puyehue-Cordón Caulle y su posible impacto ambiental: Insecticidal properties of ashes from the volcanic complex Puyehue-Caulle Range and their possible environmental impact

    Directory of Open Access Journals (Sweden)

    Micaela Buteler

    2011-12-01

    Full Text Available Se examina el efecto insecticida de las cenizas volcánicas en general y sus efectos ambientales, con especial énfasis en las cenizas acumuladas como consecuencia de la erupción del complejo volcánico Puyehue-Cordón Caulle, que actualmente afecta un área de más de 7,5 millones de hectáreas en la Patagonia Argentina. Se determinó la composición química de las cenizas provenientes del complejo Puyehue-Cordón Caulle por EDS, y se comprobó que los componentes mayoritarios son el O, Si, Al, Fe, Na y K. A través de bioensayos de laboratorio, utilizando dos especies de insectos como modelo biológico, se determinó que las cenizas se adhieren profusamente a la superfi cie corporal y resultan tóxicas para ambas especies cuando se mezclan con el sustrato en baja concentración. La susceptibilidad a las cenizas difi rió entre ambas especies en bioensayos de siete días de exposición continua [CL99(Oryzaephilus surinamensis L. = 10.25 mg.g-1; CL99 ((Tribolium castaneum (Herbst = 4.96 mg.g-1]. De estos resultados, se desprende que el impacto de la ceniza del complejo Puyehue-Cordón Caulle sobre la comunidad de artrópodos puede ser radical en la fase aguda de la deposición, y que sus efectos podrían ser substancialmente cualitativos en la fase crónica de la exposición. Así también, la recuperación de las poblaciones afectadas por la ceniza en las zonas con clima desértico, donde persiste la ceniza seca, será más lenta, hecho que tendrá consecuencias tangibles a largo plazo para las plantas hospederas de insectos, para la polinización y sobre el desarrollo de las poblaciones de vertebrados insectívoros. En contraste, en las regiones húmedas, la presencia de cenizas representa un cambio manifi esto en el ecosistema debido al aporte de nutrientes, que en combinación con la retención de agua por las cenizas, llevará a la recuperación cuali y cuantitativa de las poblaciones bajo condiciones favorables.This paper discusses the

  15. Combating highly resistant emerging pathogen Mycobacterium abscessus and Mycobacterium tuberculosis with novel salicylanilide esters and carbamates.

    Science.gov (United States)

    Baranyai, Zsuzsa; Krátký, Martin; Vinšová, Jarmila; Szabó, Nóra; Senoner, Zsuzsanna; Horváti, Kata; Stolaříková, Jiřina; Dávid, Sándor; Bősze, Szilvia

    2015-08-28

    In the Mycobacterium genus over one hundred species are already described and new ones are periodically reported. Species that form colonies in a week are classified as rapid growers, those requiring longer periods (up to three months) are the mostly pathogenic slow growers. More recently, new emerging species have been identified to lengthen the list, all rapid growers. Of these, Mycobacterium abscessus is also an intracellular pathogen and it is the most chemotherapy-resistant rapid-growing mycobacterium. In addition, the cases of multidrug-resistant Mycobacterium tuberculosis infection are also increasing. Therefore there is an urgent need to find new active molecules against these threatening strains. Based on previous results, a series of salicylanilides, salicylanilide 5-chloropyrazinoates and carbamates was designed, synthesized and characterised. The compounds were evaluated for their in vitro activity on M. abscessus, susceptible M. tuberculosis H37Rv, multidrug-resistant (MDR) M. tuberculosis MDR A8, M. tuberculosis MDR 9449/2006 and on the extremely-resistant Praha 131 (XDR) strains. All derivatives exhibited a significant activity with minimum inhibitory concentrations (MICs) in the low micromolar range. Eight salicylanilide carbamates and two salicylanilide esters exhibited an excellent in vitro activity on M. abscessus with MICs from 0.2 to 2.1 μM, thus being more effective than ciprofloxacin and gentamicin. This finding is potentially promising, particularly, as M. abscessus is a threateningly chemotherapy-resistant species. M. tuberculosis H37Rv was inhibited with MICs from 0.2 μM, and eleven compounds have lower MICs than isoniazid. Salicylanilide esters and carbamates were found that they were effective also on MDR and XDR M. tuberculosis strains with MICs ≥1.0 μM. The in vitro cytotoxicity (IC50) was also determined on human MonoMac-6 cells, and selectivity index (SI) of the compounds was established. In general, salicylanilide

  16. Ti, Al

    Indian Academy of Sciences (India)

    In the present study, authors report on the effect that substrate bias voltage has on the microstructure and mechanical properties of (Ti, Al)N hard coatings deposited with cathodic arc evaporation (CAE) technique. The coatings were deposited from a Ti0.5Al0.5 powder metallurgical target in a reactive nitrogen atmosphere at ...

  17. Efecto de las enmiendas básicas sobre el complejo de cambio en algunos suelos ácidos de la Región Pampeana Application of basic amendments on acid soils of the Pampa Region: effect on the soil exchange complex

    Directory of Open Access Journals (Sweden)

    Guillermo Millán

    2010-12-01

    Full Text Available La acidez edáfica causa problemáticas productivas derivadas de disturbios microbiológicos, aspectos nutricionales y hasta fitotoxicidad de Al3+. El objetivo de este trabajo es: a evaluar la capacidad de intercambio catiónico y dotación de nutrientes básicos de algunos suelos ácidos del ámbito de la Pradera Pampeana; b cuantificar el Al3+ de su solución interna; c evaluar la incidencia de enmiendas básicas sobre el complejo de cambio. Se analizó el pH actual/potencial, el complejo de cambio y Al3+ intercambiable en 10 suelos Argiudoles y Hapludoles seleccionados por su acidez. Adicionalmente se evaluó la capacidad de intercambio catiónico a cada nivel de pH del suelo. Los suelos estudiados presentaron deficiencias relativas de Ca+2, en relación a Mg+2 y K+, y en menor medida de Mg+2 en relación al K+. Los valores de pH actual entre fuerte/ligeramente ácidos, se correspondieron con un pH potencial entre muy fuerte/ medianamente ácido. El encalado propició el aumento de la capacidad de intercambio catiónico y el Ca intercambiable. Si bien el Al3+ intercambiable no alcanzó niveles de toxicidad, el agregado de cualquier combinación de corrector y dosis fue eficiente para disminuirlo entre 4 y 5 veces, respecto de la situación original. El yeso no modificó el efecto de la caliza sobre el Al3+, pero su adición a la dolomita redujo la eficiencia del carbonato. Las dosis más eficientes para reducir el contenido de Al3+ intercambiable fueron las de 1.500 y 2.000 kg ha-1, dentro de cada corrector.Edaphic acidity causes productivity problems due to microbiological and nutritional disturbances and Al+3 phytotoxicity. The purpose of this study was to: a evaluate the cation exchange capacity and the amount of basic nutrients present in some acid soils of the Pampa Region, b evaluate the exchangeable Al3+ concentration, and c assess the effect of different rates and types of alkaline amendments on the exchange complex. Real and

  18. Prevalence of latent Mycobacterium tuberculosis infection in prisoners.

    Science.gov (United States)

    Navarro, Pedro Daibert de; Almeida, Isabela Neves de; Kritski, Afrânio Lineu; Ceccato, Maria das Graças; Maciel, Mônica Maria Delgado; Carvalho, Wânia da Silva; Miranda, Silvana Spindola de

    2016-01-01

    To determine the prevalence of and the factors associated with latent Mycobacterium tuberculosis infection (LTBI) in prisoners in the state of Minas Gerais, Brazil. This was a cross-sectional cohort study conducted in two prisons in Minas Gerais. Tuberculin skin tests were performed in the individuals who agreed to participate in the study. A total of 1,120 individuals were selected for inclusion in this study. The prevalence of LTBI was 25.2%. In the multivariate analysis, LTBI was associated with self-reported contact with active tuberculosis patients within prisons (adjusted OR = 1.51; 95% CI: 1.05-2.18) and use of inhaled drugs (adjusted OR = 1.48; 95% CI: 1.03-2.13). Respiratory symptoms were identified in 131 (11.7%) of the participants. Serological testing for HIV was performed in 940 (83.9%) of the participants, and the result was positive in 5 (0.5%). Two cases of active tuberculosis were identified during the study period. Within the prisons under study, the prevalence of LTBI was high. In addition, LTBI was associated with self-reported contact with active tuberculosis patients and with the use of inhaled drugs. Our findings demonstrate that it is necessary to improve the conditions in prisons, as well as to introduce strategies, such as chest X-ray screening, in order to detect tuberculosis cases and, consequently, reduce M. tuberculosis infection within the prison system. Determinar a prevalência e os fatores associados à infecção latente por Mycobacterium tuberculosis (ILTB) em pessoas privadas de liberdade no Estado de Minas Gerais. Estudo de coorte transversal realizado em duas penitenciárias em Minas Gerais. Foi realizada a prova tuberculínica nos indivíduos que aceitaram participar do estudo. Foram selecionados 1.120 indivíduos para a pesquisa. A prevalência da ILTB foi de 25,2%. Na análise multivariada, a ILTB esteve associada com relato de contato com caso de tuberculose ativa dentro da penitenciária (OR ajustada = 1,51; IC95%: 1

  19. Analysis of the leprosy agents Mycobacterium leprae and Mycobacterium lepromatosis in four countries.

    Science.gov (United States)

    Han, Xiang Y; Aung, Fleur M; Choon, Siew Eng; Werner, Betina

    2014-10-01

    To differentiate the leprosy agents Mycobacterium leprae and Mycobacterium lepromatosis and correlate them with geographic distribution and clinicopathologic features. Species-specific polymerase chain reactions were used to detect each bacillus in archived skin biopsy specimens from patients with leprosy from Brazil (n = 52), Malaysia (n = 31), Myanmar (n = 9), and Uganda (n = 4). Findings were correlated with clinical and pathologic data. Etiologic species was detected in 46 of the 52 Brazilian patients, including 36 patients with M leprae, seven with M lepromatosis, and three with both bacilli. The seven patients with sole M lepromatosis all had tuberculoid leprosy, whereas only nine of the 36 patients infected with M leprae exhibited this type, and the rest were lepromatous (P leprae and two with M lepromatosis. Of the Malaysian and Ugandan patients, only M leprae was detected in 27 of the 31 Malaysians and two of the four Ugandans. The leprosy agents vary in geographic distribution. Finding M lepromatosis in Brazil and Myanmar suggests wide existence of this newly discovered species. The leprosy manifestations likely vary with the etiologic agents. Copyright© by the American Society for Clinical Pathology.

  20. Retos de la Universidad de Guantánamo en el proceso de innovación social conectado al desarrollo local

    Directory of Open Access Journals (Sweden)

    Rolando Durand-Rill

    2017-04-01

    Full Text Available Se sistematiza la experiencia de la Universidad de Guantánamo (UG como interfase innovadora e integrada al desarrollo local, se argumenta la necesidad de fortalecer el empoderamiento ciudadano en la toma de decisiones ante los complejos retos actuales para la solución creativa de problemas, y se proponen acciones en el tratamiento de la innovación social, vinculadas con las líneas de investigación universitaria.

  1. [Ecology and transmission of Mycobacterium ulcerans].

    Science.gov (United States)

    Marsollier, L; Aubry, J; Saint-André, J-P; Robert, R; Legras, P; Manceau, A-L; Bourdon, S; Audrain, C; Carbonnelle, B

    2003-10-01

    Mycobacterium ulcerans is an environmental pathogen concerning mainly the tropical countries; it is the causative agent of Buruli ulcer, which has become the third most important mycobacterial disease. In spite of water-linked epidemiological studies to identify the sources of M. ulcerans, the reservoir and the mode of transmission of this organism remain elusive. To determine the ecology and the mode of transmission of M. ulcerans we have set up an experimental model. This experimental model demonstrated that water bugs were able to transmit M. ulcerans by bites. In insects, the bacilli were localized exclusively within salivary glands, where it could both multiply contrary to other mycobacteria species. In another experimental study, we report that the crude extracts from aquatic plants stimulate in vitro the growth of M. ulcerans as much as the biofilm formation by M. ulcerans has been observed on aquatic plants. Given that the water bugs are essentially carnivorous, it is difficult to imagine a direct contact in the contamination of aquatic bugs and plants. It seems very likely that an intermediate host exists. In an endemic area of Daloa in Côte d'Ivoire, our observations were confirmed.

  2. DNA repair in Mycobacterium tuberculosis revisited.

    Science.gov (United States)

    Dos Vultos, Tiago; Mestre, Olga; Tonjum, Tone; Gicquel, Brigitte

    2009-05-01

    Our understanding of Mycobacterium tuberculosis DNA repair mechanisms is still poor compared with that of other bacterial organisms. However, the publication of the first complete M. tuberculosis genome sequence 10 years ago boosted the study of DNA repair systems in this organism. A first step in the elucidation of M. tuberculosis DNA repair mechanisms was taken by Mizrahi and Andersen, who identified homologs of genes involved in the reversal or repair of DNA damage in Escherichia coli and related organisms. Genes required for nucleotide excision repair, base excision repair, recombination, and SOS repair and mutagenesis were identified. Notably, no homologs of genes involved in mismatch repair were identified. Novel characteristics of the M. tuberculosis DNA repair machinery have been found over the last decade, such as nonhomologous end joining, the presence of Mpg, ERCC3 and Hlr - proteins previously presumed to be produced exclusively in mammalian cells - and the recently discovered bifunctional dCTP deaminase:dUTPase. The study of these systems is important to develop therapeutic agents that can counteract M. tuberculosis evolutionary changes and to prevent adaptive events resulting in antibiotic resistance. This review summarizes our current understanding of the M. tuberculosis DNA repair system.

  3. Characterization of Mycobacterium tuberculosis nicotinamidase/pyrazinamidase.

    Science.gov (United States)

    Zhang, Hua; Deng, Jiao-Yu; Bi, Li-Jun; Zhou, Ya-Feng; Zhang, Zhi-Ping; Zhang, Cheng-Gang; Zhang, Ying; Zhang, Xian-En

    2008-02-01

    The nicotinamidase/pyrazinamidase (PncA) of Mycobacterium tuberculosis is involved in the activation of the important front-line antituberculosis drug pyrazinamide by converting it into the active form, pyrazinoic acid. Mutations in the pncA gene cause pyrazinamide resistance in M. tuberculosis. The properties of M. tuberculosis PncA were characterized in this study. The enzyme was found to be a 20.89 kDa monomeric protein. The optimal pH and temperature of enzymatic activity were pH 7.0 and 40 degrees C, respectively. Inductively coupled plasma-optical emission spectrometry revealed that the enzyme was an Mn(2+)/Fe(2+)-containing protein with a molar ratio of [Mn(2+)] to [Fe(2+)] of 1 : 1; furthermore, the external addition of either type of metal ion had no apparent effect on the wild-type enzymatic activity. The activity of the purified enzyme was determined by HPLC, and it was shown that it possessed similar pyrazinamidase and nicotinamidase activity, by contrast with previous reports. Nine PncA mutants were generated by site-directed mutagenesis. Determination of the enzymatic activity and metal ion content suggested that Asp8, Lys96 and Cys138 were key residues for catalysis, and Asp49, His51, His57 and His71 were essential for metal ion binding. Our data show that M. tuberculosis PncA may bind metal ions in a manner different from that observed in the case of Pyrococcus horikoshii PncA.

  4. The transcriptional regulatory network of Mycobacterium tuberculosis.

    Directory of Open Access Journals (Sweden)

    Joaquín Sanz

    Full Text Available Under the perspectives of network science and systems biology, the characterization of transcriptional regulatory (TR networks beyond the context of model organisms offers a versatile tool whose potential remains yet mainly unexplored. In this work, we present an updated version of the TR network of Mycobacterium tuberculosis (M.tb, which incorporates newly characterized transcriptional regulations coming from 31 recent, different experimental works available in the literature. As a result of the incorporation of these data, the new network doubles the size of previous data collections, incorporating more than a third of the entire genome of the bacterium. We also present an exhaustive topological analysis of the new assembled network, focusing on the statistical characterization of motifs significances and the comparison with other model organisms. The expanded M.tb transcriptional regulatory network, considering its volume and completeness, constitutes an important resource for diverse tasks such as dynamic modeling of gene expression and signaling processes, computational reliability determination or protein function prediction, being the latter of particular relevance, given that the function of only a small percent of the proteins of M.tb is known.

  5. Lipid Droplets and Mycobacterium leprae Infection

    Science.gov (United States)

    Elamin, Ayssar A.; Stehr, Matthias; Singh, Mahavir

    2012-01-01

    Leprosy is a chronic infectious disease and is a major source of morbidity in developing countries. Leprosy is caused by the obligate intracellular bacterium Mycobacterium leprae, which infects as primary target Schwann cells. Lepromatous leprosy exhibits multiple lesions of the skin, eyes, nerves, and lymph nodes. The sites of infection are characterized by the presence of foamy macrophages, fully packed with lipid droplets (LDs), which are induced by M. leprae. In the last years, it has become evident that M. tuberculosis imports lipids from foamy macrophages and is dependent on fatty acids for growth in infected macrophages. M. leprae seems to have similar mechanisms for scavenging lipids from the host. But due to the inability to culture M. leprae on laboratory media, research progresses only slowly. However, in the last years, substantial progress has been made in the field of lipid metabolism in M. leprae. Herein, we will present and summarize the lipid droplets formation and the metabolism of lipids during M. leprae infection. PMID:23209912

  6. The cell envelope glycoconjugates of Mycobacterium tuberculosis

    Science.gov (United States)

    Angala, Shiva Kumar; Belardinelli, Juan Manuel; Huc-Claustre, Emilie; Wheat, William H.; Jackson, Mary

    2015-01-01

    Tuberculosis (TB) remains the second most common cause of death due to a single infectious agent. The cell envelope of Mycobacterium tuberculosis (Mtb), the causative agent of the disease in humans, is a source of unique glycoconjugates and the most distinctive feature of the biology of this organism. It is the basis of much of Mtb pathogenesis and one of the major causes of its intrinsic resistance to chemotherapeutic agents. At the same time, the unique structures of Mtb cell envelope glycoconjugates, their antigenicity and essentiality for mycobacterial growth provide opportunities for drug, vaccine, diagnostic and biomarker development, as clearly illustrated by recent advances in all of these translational aspects. This review focuses on our current understanding of the structure and biogenesis of Mtb glycoconjugates with particular emphasis on one of most intriguing and least understood aspect of the physiology of mycobacteria: the translocation of these complex macromolecules across the different layers of the cell envelope. It further reviews the rather impressive progress made in the last ten years in the discovery and development of novel inhibitors targeting their biogenesis. PMID:24915502

  7. Phenotypic assays for Mycobacterium tuberculosis infection.

    Science.gov (United States)

    Song, Ok-Ryul; Deboosere, Nathalie; Delorme, Vincent; Queval, Christophe J; Deloison, Gaspard; Werkmeister, Elisabeth; Lafont, Frank; Baulard, Alain; Iantomasi, Raffaella; Brodin, Priscille

    2017-10-01

    Tuberculosis (TB) is still a major global threat, killing more than one million persons each year. With the constant increase of Mycobacterium tuberculosis strains resistant to first- and second-line drugs, there is an urgent need for the development of new drugs to control the propagation of TB. Although screenings of small molecules on axenic M. tuberculosis cultures were successful for the identification of novel putative anti-TB drugs, new drugs in the development pipeline remains scarce. Host-directed therapy may represent an alternative for drug development against TB. Indeed, M. tuberculosis has multiple specific interactions within host phagocytes, which may be targeted by small molecules. In order to enable drug discovery strategies against microbes residing within host macrophages, we developed multiple fluorescence-based HT/CS phenotypic assays monitoring the intracellular replication of M. tuberculosis as well as its intracellular trafficking. What we propose here is a population-based, multi-parametric analysis pipeline that can be used to monitor the intracellular fate of M. tuberculosis and the dynamics of cellular events such as phagosomal maturation (acidification and permeabilization), zinc poisoning system or lipid body accumulation. Such analysis allows the quantification of biological events considering the host-pathogen interplay and may thus be derived to other intracellular pathogens. © 2017 International Society for Advancement of Cytometry. © 2017 International Society for Advancement of Cytometry.

  8. Inactivation of Mycobacterium avium with free chlorine.

    Science.gov (United States)

    Luh, Jeanne; Mariñas, Benito J

    2007-07-15

    The inactivation kinetics of Mycobacterium avium with free chlorine was characterized by two stages: an initial phase at a relatively fast rate followed by a slower second stage of pseudo first-order kinetics. The inactivation rate of each stage was approximately the same for all experiments performed at a certain condition of pH and temperature; however, variability was observed for the disinfectant exposure at which the transition between the two stages occurred. This variability was not a function of the initial disinfectant concentration, the initial bacterial density, or the bacterial stock. However, the transition to the second stage varied more significantly at high temperatures (30 degrees C), while lower variability was observed at lower temperatures (5 and 20 degrees C). Experiments conducted at pH values in the range of 6-9 revealed that the inactivation of M. avium was primarily due to hypochlorous acid, with little contribution from hypochlorite ion within this pH range. The inactivation kinetics was represented with a two-population model. The activation energies for the resulting pseudo first-order rate constants for the populations with fast and slow kinetics were 100.3 and 96.5 kJ/mol, respectively. The magnitude of these values suggested that for waters of relatively high pH and low temperatures, little inactivation of M. avium would be achieved within treatment plants, providing a seeding source for distribution systems.

  9. Variación espacial y temporal en los contenidos de mercurio, plomo, cromo y materia orgánica en sedimento del complejo de humedales de Ayapel, Córdoba, noroccidente colombiano

    Directory of Open Access Journals (Sweden)

    Alex Fernando Rúa Cardona

    2013-01-01

    Full Text Available Las planicies de inundación atlánticas colombianas están impactadas por la minería aurífera artesanal desarrollada durante las últimas décadas. Sin embargo, la contaminación local por mercurio ha sido comparada con niveles de acción de otras latitudes y no se han incluido otros metales de interés sanitario en las investigaciones. El presente estudio contribuyó al conocimiento de la respuesta geoquímica espacial inducida por el pulso hidrológico y la composición de materia orgánica (MO en el área de influencia del complejo de humedales de Ayapel. Se evaluó la distribución espacial y temporal de mercurio (Hg, plomo (Pb, cromo (Cr y MO en el sedimento reciente del complejo cenagoso de Ayapel y se contrastó con el nivel de referencia de contaminación local por Hg (0,032 μg g-1, Pb (2,39 μg g-1 y Cr (89,58 μg g-1 determinado en la Formación Betulia. Los rangos de los tres metales fueron: 0,008-0,117 μg Hg g-1; 1,090-11,870 μg Pb g-1 y 5,350- 93,430 μg Cr g-1. El Hg mostró la mayor variabilidad espacial (0,083 ± 0,064 μg g-1 y niveles superiores en el afluente del frente de explotación aurífera en la década del 80. En comparación con el nivel de referencia, el 91 % del sedimento reciente presentó enriquecimiento por Pb (6,376 ± 4,048 μg g-1 y el 66,6 % enriquecimiento por Hg. En contraste, el contenido de Cr fue relativamente bajo (96,325 ± 29,868 μg g-1 y presentó tendencia a disminuir de oriente a occidente. El sedimento fue predominantemente inorgánico, con porcentajes medios de cenizas de 88,154 ± 3,822 % y escasos niveles de MO (11,846 ± 3,822 %. Se presume el aumento en la movilidad del Hg debido a la alta tasa de respiración de MO en la zona central del espejo de agua principal de la ciénaga de Ayapel. La resuspensión y migración de sedimentos contaminados es facilitada por la inestabilidad térmica de la columna de agua influenciada por la acción del viento.

  10. Mycobacterium abscessus skin infection after tattooing - Case report*

    Science.gov (United States)

    de Sousa, Pétra Pereira; Cruz, Rossilene Conceição da Silva; Schettini, Antonio Pedro Mendes; Westphal, Danielle Cristine

    2015-01-01

    Mycobacterium abscessus is a rapidly growing mycobacterium that has been affecting people undergoing invasive procedures, such as videosurgery and mesotherapy. This bacterium has global distribution, being found in numerous niches. The frequency of published reports of infection by rapidly growing mycobacteria associated with tattooing procedures has increased in recent years. However, in Brazil there were no case reports of M. abscessus after tattooing in the literature until now. In this paper, we describe the case of a patient with a nine-month history of lesion on a tattoo site. The diagnosis of infection with Mycobacterium abscessus was established by correlation between dermatological and histopathological aspects, culture and molecular biology techniques. The patient had significant improvement of symptoms with the use of clarithromycin monotherapy. PMID:26560222

  11. MYCOBACTERIUM GENAVENSE IN AN AFRICAN PENGUIN (SPHENISCUS DEMERSUS).

    Science.gov (United States)

    Krause, Kristian J; Reavill, Drury; Weldy, Scott H; Bradway, Daniel S

    2015-12-01

    A 19-yr-old female African penguin (Spheniscus demersus) presented with labored breathing and anorexia. Radiographs revealed soft-tissue density lesions in the left lung fields and fluid in the right. The penguin died during the night. Postmortem examination demonstrated multiple granulomas in the lungs and air sacs. The right coelom was filled with opaque fluid. Histopathology of the lung, liver, kidney, and spleen identified Mycobacterium as a primary disease etiology. Large numbers of acid fast-positive, rod-shaped bacteria were recognized on tissue staining. Mycobacterium genavense was detected by polymerase chain reaction (PCR) using primers specific for the species. Further confirmation of M. genavense was accomplished using PCR with universal Mycobacterium spp. primers followed by sequencing of the amplicon obtained. To our knowledge, this is the first reported case of mycobacteriosis-and specifically M. genavense -in an African penguin. This case also demonstrates the similarities of presentation between the more commonly suspected and encountered aspergillosis and mycobacteriosis.

  12. Imaging features of mycobacterium in patients with acquired immunodeficiency syndrome

    International Nuclear Information System (INIS)

    Yang Jun; Sun Yue; Wei Liangui; Xu Yunliang; Li Xingwang

    2013-01-01

    Objective: To analyze the imaging features of mycobacterium in AIDS patients. Methods: Twenty-three cases of mycobacterium tuberculosis and 13 patients of non-tuberculous mycobacteria were proved etiologically and included in this study. All patients underwent X-ray and CT examinations, imaging data were analyzed and compared. Results: The imaging findings of mycobacterium tuberculosis in AIDS patients included consolidation (n = 11), pleural effusion (n = 11), mediastinal lymphadenopathy (n = 11). Pulmonary lesions were always diffuse distribution, and 14 patients of extrapulmonary tuberculosis were found. Pulmonary lesions in non-tuberculous mycobacteria tend to be circumscribed. Conclusions: Non-tuberculous mycobacterial infection in AIDS patients is more common and usually combined with other infections. Imaging features are atypical. (authors)

  13. Serovars of Mycobacterium avium Complex isolated from patients in Denmark

    DEFF Research Database (Denmark)

    Askgaard, D. S.; Giese, Steen Bjørck; Thybo, S.

    1994-01-01

    Danish isolates of Mycobacterium avium complex were serotyped by the use of seroagglutination. The most prevalent serovars among patients with AIDS (n = 89) were 4 and 6, while among non-AIDS patients the most prevalent serovars were 1, 6, and 4, with no major differences between those in patients...... with pulmonary disease (n = 65) and those in patients with lymph node infection (n = 58). The results suggest a Scandinavian distribution of serovars with a predominance of serovar 6 and fail to demonstrate any selective protection against different serovars by Mycobacterium bovis ECG vaccination....

  14. Radiographic differentiation of atypical tuberculosis from mycobacterium tuberculosis

    International Nuclear Information System (INIS)

    Tarver, R.D.; Pearcy, E.A.; Conces, D.J. Jr.; Mathur, P.N.

    1987-01-01

    The chest radiographs of 95 patients with the new diagnosis of atypical turberculosis were reviewed to determine if any significant differences between atypical tuberculosis and that caused by Mycobacterium tuberculosis could be discerned. Findings included upper lobe involvement in B4 of the 95 patients and cavities in 76, with nearly equal groups having no, moderate, or extensive surrounding alveolar disease. Nodules were common; in six patients a nodule was the sole manifestation of disease. Adenopathy was seen in 12 of the 95 patients, atlectasis in 45, pleural thickening in 90, and effusions in three. These radiographic findings did not allow the radiographic differentiation of atypical tuberculosis from Mycobacterium tuberculosis infection

  15. ESTUDIO DEL EFECTO DE ISOTÓPO DE HIDRÓGENO EN LOS COMPLEJOS M–H•••H–F (M=Li, Na

    Directory of Open Access Journals (Sweden)

    Andrés Reyes

    2009-06-01

    Full Text Available Se estudió teóricamente el efecto de isotópo de hidrógeno sobre la geometría, la distribución de carga electrónica, la estabilidad relativa y la energía de formación de complejos lineales tipo M–X···Y–F y todos sus isotopólogos de hidrógeno (M=Li, Na; X, Y= H, D, T. Estos estudios fueron realizados con el paquete computacional APMO a un nivel de teoría Hartree-Fock electrónico y nuclear. Los resultados obtenidos están de acuerdo con resultados reportados por otros autores que usan métodos de estructura electrónica convencional.  

  16. Reacción de ligandos azufrados con el complejo au2pt (ch2p(Sph24,cl2

    Directory of Open Access Journals (Sweden)

    Guillermo Garzón

    2010-07-01

    Full Text Available Se describe la reacción de intercambio del cloro en el complejo au2pt (ch2p(Sph24,cl2 por los ligandos SCH2Ph-,S2CN(CH2CH3,2- y SCN-. Los nuevos derivados azufrados se caracterizan por análisis químico, espectroscopia infrarroja, electrónica y resonancia magnética nuclear protónica. Los ligandos se coordinan por el azufre a los dos átomos sw oro metálico en forma simétrica. El análisis elemental concuerda con las estequiomctrías y estructuras propuestas.

  17. Variación genética en el complejo infraespecífico de chayote evaluada mediante sistemas isoenzimáticos

    Directory of Open Access Journals (Sweden)

    Carlos Hugo Avendaño-Arrazate

    2012-02-01

    Full Text Available El objetivo de este trabajo fue estimar el grado de variación genética dentro del complejo infraespecífico de Sechium mediante el uso de sistemas isoenzimáticos. Se analizaron 23 loci codificados por 12 sistemas isoenzimáticos, en geles de almidón, en 10 individuos de cada una de las 30 accesiones (27 cultivadas y tres silvestres. La variación genética se estimó con base en el número promedio de alelos por locus (NPAL, porcentaje de porlimorfismo (PP, heterocigosidad observada y esperada (Ho y He, índice relativo de heterocigosidad (IRH e índice de Shannon (IS. Para NPAL y PP, el promedio para las 30 accesiones fue de 2, 03 y 59, 8%, respectivamente. El análisis de Ho y He mostró variación genética en el complejo infraespecífico de Sechium, con valores promedio de 0, 05 y 0, 26, respectivamente. El IRH mostró una deficiencia de individuos heterocigotos (promedio de -0, 75. El IS mostró gran diversidad en las 30 accesiones (0, 41. Las poblaciones con mayor diversidad fueron Negrito, Verde liso, Negro xalapa, Verde espinoso y Negro cónico; con una variación intermedia fueron Castilla blanco, Caldero y Blanco pequeño; y, con poca variación, Castilla verde, Cambray y los parientes silvestres.

  18. Immune Responses Involved in Mycobacterium Tuberculosis Infection

    Directory of Open Access Journals (Sweden)

    Roghayeh Teimourpour

    2016-09-01

    Full Text Available Background and Objectives: Mycobacterium tuberculosis is the causative agent of tuberculosis (TB. Approximately one-third of the world's population is infected with M. tuberculosis. Despite the availability of drug and vaccine, it remains one of the leading causes of death in humans especially in developing countries. Epidemiological studies have indicated that only 10-30% of people exposed to tubercle bacillus are infected with M. tuberculosis, and at least 90% of the infected people finally do not acquire TB. The studies have indicated that the host efficient immune system has essential roles in the control of TB infection such that the highest rate of mortality and morbidity is seen in immunocompromised patients such as people infected with HIV. M. tuberculosis is an obligatory intracellular bacterium. It enters the body mainly through the respiratory tract and alveolar macrophages combat this pathogen most commonly. In addition to alveolar macrophages, various T-cell subpopulations need to be activated to overcome this bacterium's resistance to the host defense systems. CD4+ T cells, through production of several cytokines such as IFN-γ and TNF-α, and CD8+ T cells, through cytotoxic activities and induction of apoptosis in infected cells, play critical roles in inducing appropriate immune responses against M. tuberculosis. Although cell-mediated immunity is the cornerstone of host responses against TB and the recent studies have provided evidence for the importance of humoral and innate immune system in the control of TB, a profound understanding of the immune responses would provide a basis for development of new generations of vaccines and drugs. The present study addresses immune responses involved in M. tuberculosis infection.

  19. Nicotine Impairs Macrophage Control of Mycobacterium tuberculosis.

    Science.gov (United States)

    Bai, Xiyuan; Stitzel, Jerry A; Bai, An; Zambrano, Cristian A; Phillips, Matthew; Marrack, Philippa; Chan, Edward D

    2017-09-01

    Pure nicotine impairs macrophage killing of Mycobacterium tuberculosis (MTB), but it is not known whether the nicotine component in cigarette smoke (CS) plays a role. Moreover, the mechanisms by which nicotine impairs macrophage immunity against MTB have not been explored. To neutralize the effects of nicotine in CS extract, we used a competitive inhibitor to the nicotinic acetylcholine receptor (nAChR)-mecamylamine-as well as macrophages derived from mice with genetic disruption of specific subunits of nAChR. We also determined whether nicotine impaired macrophage autophagy and whether nicotine-exposed T regulatory cells (Tregs) could subvert macrophage anti-MTB immunity. Mecamylamine reduced the CS extract increase in MTB burden by 43%. CS extract increase in MTB was also significantly attenuated in macrophages from mice with genetic disruption of either the α7, β2, or β4 subunit of nAChR. Nicotine inhibited autophagosome formation in MTB-infected THP-1 cells and primary murine alveolar macrophages, as well as increased the intracellular MTB burden. Nicotine increased migration of THP-1 cells, consistent with the increased number of macrophages found in the lungs of smokers. Nicotine induced Tregs to produce transforming growth factor-β. Naive mouse macrophages co-cultured with nicotine-exposed Tregs had significantly greater numbers of viable MTB recovered with increased IL-10 production and urea production, but no difference in secreted nitric oxide as compared with macrophages cocultured with unexposed Tregs. We conclude that nicotine in CS plays an important role in subverting macrophage control of MTB infection.

  20. Search for Mycobacterium leprae in wild mammals

    Directory of Open Access Journals (Sweden)

    Sílvia Cristina Barboza Pedrini

    Full Text Available Leprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus sexcinctus, Cabassous unicinctus, and C. tatouay armadillos from the Middle Western region of the state of São Paulo and from the experimental station of Embrapa Pantanal, located in Pantanal da Nhecolândia of Mato Grosso do Sul state. Also, the molecular analysis of 19 samples from internal organs of other road killed species of wild animals, such as Nasua nasua (ring-tailed coati, Procyon cancrivoros (hand-skinned, Cerdocyon thous (dog-pity-bush, Cavia aperea (restless cavy, Didelphis albiventris (skunk, Sphigurrus spinosus (hedgehog, and Gallictis vittata (ferret showed PCR negative data. None of the 157 analyzed samples had shown natural mycobacterial infection. Only the armadillo inoculated with material collected from untreated multibacillary leprosy patient presented PCR positive and its genomic sequencing revealed 100% identity with M. leprae. According to these preliminary studies, based on the used methodology, it is possible to conclude that wild mammals seem not to play an important role in the epidemiology of leprosy in the Middle Western region of the São Paulo state and in the Pantanal of Mato Grosso do Sul state.

  1. Anti-mycobacterium tuberculosis activity of polyherbal medicines used for the treatment of tuberculosis in Eastern Cape, South Africa.

    Science.gov (United States)

    Famewo, Elizabeth B; Clarke, Anna M; Wiid, Ian; Ngwane, Andile; van Helden, Paul; Afolayan, Anthony J

    2017-09-01

    The emergence of drug-resistant strains of Mycobacterium tuberculosis has become a global public health problem. Polyherbal medicines offer great hope for developing alternative drugs for the treatment of tuberculosis. To evaluate the anti-tubercular activity of polyherbal medicines used for the treatment of tuberculosis. The remedies were screened against Mycobacterium tuberculosis H37Rv using Middlebrook 7H9 media and MGIT BACTEC 960 system. They were liquid preparations from King Williams Town site A (KWTa), King Williams Town site B (KWTb), King Williams Town site C (KWTc), Hogsback first site (HBfs), Hogsback second site (HBss), Hogsback third site (HBts), East London (EL), Alice (AL) and Fort Beaufort (FB). The susceptibility testing revealed that all the remedies contain anti-tubercular activity with KWTa, KWTb, KWTc, HBfs, HBts, AL and FB exhibiting more activity at a concentration below 25 µl/ml. Furthermore, MIC values exhibited inhibitory activity with the most active remedies from KWTa, HBfs and HBts at 1.562 µg/ml. However, isoniazid showed more inhibitory activity against M. tuberculosis at 0.05 µg/ml when compare to the polyherbal remedies. This study has indicated that these remedies could be potential sources of new anti-mycobacterial agents against M. tuberculosis . However, the activity of these preparations and their active principles still require in vivo study in order to assess their future as new anti-tuberculosis agents.

  2. Mycobacterium avium subspecies paratuberculosis: A possible ...

    African Journals Online (AJOL)

    Ibrahim Eldaghayes

    2018-05-04

    May 4, 2018 ... of both water and biofilm samples from 31 cold water ... temperatures ranging from 15 to 45°C and salinities .... et al., 2005) couple with specific growth requirements such as ..... paratuberculosis in muscle: lymphatic and organ.

  3. Expression of Mycobacterium smegmatis pyrazinamidase in Mycobacterium tuberculosis confers hypersensitivity to pyrazinamide and related amides.

    Science.gov (United States)

    Boshoff, H I; Mizrahi, V

    2000-10-01

    A pyrazinamidase (PZase)-deficient pncA mutant of Mycobacterium tuberculosis, constructed by allelic exchange, was used to investigate the effects of heterologous amidase gene expression on the susceptibility of this organism to pyrazinamide (PZA) and related amides. The mutant was highly resistant to PZA (MIC, >2,000 microg/ml), in accordance with the well-established role of pncA in the PZA susceptibility of M. tuberculosis (A. Scorpio and Y. Zhang, Nat. Med. 2:662-667, 1996). Integration of the pzaA gene encoding the major PZase/nicotinamidase from Mycobacterium smegmatis (H. I. M. Boshoff and V. Mizrahi, J. Bacteriol. 180:5809-5814, 1998) or the M. tuberculosis pncA gene into the pncA mutant complemented its PZase/nicotinamidase defect. In both pzaA- and pncA-complemented mutant strains, the PZase activity was detected exclusively in the cytoplasm, suggesting an intracellular localization for PzaA and PncA. The pzaA-complemented strain was hypersensitive to PZA (MIC, /=20 microg/ml) and was also sensitive to benzamide (MIC, 20 microg/ml), unlike the wild-type and pncA-complemented mutant strains, which were highly resistant to this amide (MIC, >500 microg/ml). This finding was consistent with the observation that benzamide is hydrolyzed by PzaA but not by PncA. Overexpression of PzaA also conferred sensitivity to PZA, nicotinamide, and benzamide on M. smegmatis (MIC, 150 microg/ml in all cases) and rendered Escherichia coli hypersensitive for growth at low pH.

  4. Effect of chlorine on Mycobacterium gordonae and Mycobacterium chubuense in planktonic and Biofilm State

    Directory of Open Access Journals (Sweden)

    Alejandra Soledad Oriani

    2018-01-01

    Full Text Available Background: There is evidence that drinking water could be a source of infections with pathogenic nontuberculous mycobacteria (NTM potentially risky to human health. The aim was to investigate the resistance of two NTM isolated from drinking water, Mycobacterium gordonae and Mycobacterium chubuense, at different concentrations of chlorine (as sodium hypochlorite, used in drinking water sanitation. Methods: The NTM were grown in suspension and in biofilms and were challenged with biocide for 10 and 60 min. Results: To obtain 7-log reduction from the initial population of M. chubuense, in the planktonic state, there were necessary 20 ppm of chorine and 60 min of exposure. The same effect was achieved in M. gordonae with 10 ppm for the same period. The maximum reduction of both NTM in biofilm was 3-log reduction and was achieved using 30 ppm for 60 min. The chlorine susceptibility of cells in biofilms was significantly lower than that of planktonic cells. The results highlight the resistance of both NTM to the concentrations used in routine water sanitation (0.2 ppm according to Argentine Food Code. Differences in chlorine resistance found between the two NTM in planktonic growth decrease when they are grown in biofilm. Conclusion: This suggests that current water disinfection procedures do not always achieve effective control of NTM in the public supply system, with the consequent health risk to susceptible population, and the need to take into account biofilms, because of their deep consequences in the way to analyze the survival of prokaryotic cells in different environments.

  5. Insights on the Emergence of Mycobacterium tuberculosis from the Analysis of Mycobacterium kansasii

    Science.gov (United States)

    Wang, Joyce; McIntosh, Fiona; Radomski, Nicolas; Dewar, Ken; Simeone, Roxane; Enninga, Jost; Brosch, Roland; Rocha, Eduardo P.; Veyrier, Frédéric J.; Behr, Marcel A.

    2015-01-01

    By phylogenetic analysis, Mycobacterium kansasii is closely related to Mycobacterium tuberculosis. Yet, although both organisms cause pulmonary disease, M. tuberculosis is a global health menace, whereas M. kansasii is an opportunistic pathogen. To illuminate the differences between these organisms, we have sequenced the genome of M. kansasii ATCC 12478 and its plasmid (pMK12478) and conducted side-by-side in vitro and in vivo investigations of these two organisms. The M. kansasii genome is 6,432,277 bp, more than 2 Mb longer than that of M. tuberculosis H37Rv, and the plasmid contains 144,951 bp. Pairwise comparisons reveal conserved and discordant genes and genomic regions. A notable example of genomic conservation is the virulence locus ESX-1, which is intact and functional in the low-virulence M. kansasii, potentially mediating phagosomal disruption. Differences between these organisms include a decreased predicted metabolic capacity, an increased proportion of toxin–antitoxin genes, and the acquisition of M. tuberculosis-specific genes in the pathogen since their common ancestor. Consistent with their distinct epidemiologic profiles, following infection of C57BL/6 mice, M. kansasii counts increased by less than 10-fold over 6 weeks, whereas M. tuberculosis counts increased by over 10,000-fold in just 3 weeks. Together, these data suggest that M. kansasii can serve as an image of the environmental ancestor of M. tuberculosis before its emergence as a professional pathogen, and can be used as a model organism to study the switch from an environmental opportunistic pathogen to a professional host-restricted pathogen. PMID:25716827

  6. Generalized Tuberculosis in Llamas (Lama glama) Due to Mycobacterium microti

    Science.gov (United States)

    Oevermann, A.; Pfyffer, G. E.; Zanolari, P.; Meylan, M.; Robert, N.

    2004-01-01

    Necropsy of two llamas revealed numerous caseous nodules containing abundant acid-fast bacilli (AFB) in various organs. The AFB were identified by spoligotyping as Mycobacterium microti, vole type. Infection caused by M. microti should be considered in the differential diagnosis of debilitating diseases in New World camelids. PMID:15071059

  7. Cytochemical and biological properties of Mycobacterium bovis BCG.

    Science.gov (United States)

    Slosárek, M

    1977-01-01

    It was the aim of the present communication to find a simple test for a reliable discrimination of Mycobacterium bovis BCG from Mycobacterium tuberculosis. A total of 26 BCG strains, out of them 10 Czechoslovak strains (2 lyophilized cultures of BCG of different batch, 6 strains isolated from abscesses of children after BCG-vaccination and 2 strains from fatal cases after BCG-vaccination) and 16 strains obtained from foreign laboratories, were used. Of the tested characteristics a combination of 3 tests, sensitivity to 1 microgram of 2-thiophene carbonylhydrazide (TCH), activity of 3 acylamidases (urease, nicotinamidase and pyrazinamidase) and a quantitative nitrate test, was found to be most advantageous. The Czechoslovak strains of Mycobacterium bovis BCG were fully sensitive to TCH, of the 3 acylamidases mentioned above only urease was positive and nitrate was reduced only little or not at all. On the other hand, strains of Mycobacterium tuberculosis were always resistant to TCH, had positive urease, nicotinamidase and pyrazinamidase and reduced nitrate very intensively.

  8. Mycobacterium tuberculosis infection of domesticated Asian elephants, Thailand.

    OpenAIRE

    2011-01-01

    Four Asian elephants were confirmed to be infected with Mycobacterium tuberculosis by bacterial culture, other diagnostic procedures, and sequencing of 16S–23S rDNA internal transcribed spacer region, 16S rRNA, and gyrase B gene sequences. Genotyping showed that the infectious agents originated from 4 sources in Thailand. To identify infections, a combination of diagnostic assays is essential.

  9. Mycobacterium avium subsp. paratuberculosis infection, immunology and pathology of livestock

    Science.gov (United States)

    Mycobacterium avium subsp. paratuberculosis (MAP) infection in ruminants leads to a chronic and progressive enteric disease (Johne’s disease) that results in loss of intestinal function, poor body condition, and eventual death. Transmission is primarily through a fecal-oral route in neonates but con...

  10. Mycobacterium bovis hip bursitis in a lung transplant recipient.

    Science.gov (United States)

    Dan, J M; Crespo, M; Silveira, F P; Kaplan, R; Aslam, S

    2016-02-01

    We present a report of extrapulmonary Mycobacterium bovis infection in a lung transplant recipient. M. bovis is acquired predominantly by zoonotic transmission, particularly from consumption of unpasteurized foods. We discuss epidemiologic exposure, especially as relates to the Mexico-US border, clinical characteristics, resistance profile, and treatment. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  11. Siderocalin inhibits the intracellular replication of Mycobacterium tuberculosis in macrophages

    DEFF Research Database (Denmark)

    Johnson, Erin E; Srikanth, Chittur V; Sandgren, Andreas

    2010-01-01

    Siderocalin is a secreted protein that binds to siderophores to prevent bacterial iron acquisition. While it has been shown to inhibit the growth of Mycobacterium tuberculosis (M.tb) in extracellular cultures, its effect on this pathogen within macrophages is not clear. Here, we show that sideroc...

  12. Clinical manifestations, diagnosis, and treatment of Mycobacterium haemophilum infections.

    NARCIS (Netherlands)

    Lindeboom, J.A.; Bruijnesteijn van Coppenraet, L.E.; Soolingen, D. van; Prins, J.M.; Kuijper, E.J.

    2011-01-01

    Mycobacterium haemophilum is a slowly growing acid-fast bacillus (AFB) belonging to the group of nontuberculous mycobacteria (NTM) frequently found in environmental habitats, which can colonize and occasionally infect humans and animals. Several findings suggest that water reservoirs are a likely

  13. Clinical manifestations, diagnosis, and treatment of Mycobacterium haemophilum infections

    NARCIS (Netherlands)

    Lindeboom, J.A.; Bruijnesteijn van Coppenraet, L.E.S.; van Soolingen, D.; Prins, J.M.; Kuijper, E.J.

    2011-01-01

    Mycobacterium haemophilum is a slowly growing acid-fast bacillus (AFB) belonging to the group of nontuberculous mycobacteria (NTM) frequently found in environmental habitats, which can colonize and occasionally infect humans and animals. Several findings suggest that water reservoirs are a likely

  14. Sensitivity of Mycobacterium bovis to common beef processing interventions

    Science.gov (United States)

    Objective. Mycobacterium bovis is the causative agent of bovine tuberculosis, a relevant zoonosis that can spread to humans through inhalation or by ingestion. M. bovis multiplies slowly, so infected animals may be sent to slaughter during the early stages of the disease before diagnosis and when ...

  15. Polymerase chain reaction for the detection of Mycobacterium leprae

    NARCIS (Netherlands)

    Hartskeerl, R. A.; de Wit, M. Y.; Klatser, P. R.

    1989-01-01

    A polymerase chain reaction (PCR) using heat-stable Taq polymerase is described for the specific detection of Mycobacterium leprae, the causative agent of leprosy. A set of primers was selected on the basis of the nucleotide sequence of a gene encoding the 36 kDa antigen of M. leprae. With this set

  16. Transmission of Mycobacterium tuberculosis Undetected by Tuberculin Skin Testing

    Czech Academy of Sciences Publication Activity Database

    Anderson, S. T.; Williams, A. J.; Brown, J. R.; Newton, S. M.; Šimšová, Marcela; Nicol, M. P.; Šebo, Peter; Levin, M.; Wilkinson, R. J.; Wilkinson, K. A.

    2006-01-01

    Roč. 173, - (2006), s. 1038-1042 ISSN 1073-449X R&D Projects: GA AV ČR IAA5020406 Institutional research plan: CEZ:AV0Z50200510 Keywords : adenylate cyclase * diagnostic tests and procedures * mycobacterium tuberculosis Subject RIV: EE - Microbiology, Virology Impact factor: 9.091, year: 2006

  17. Microevolution of Mycobacterium tuberculosis in a tuberculosis patient.

    NARCIS (Netherlands)

    Al-Hajoj, S.A.; Akkerman, O.; Parwati, I.; Al-Gamdi, S.; Rahim, Z.; Soolingen, D. van; Ingen, J. van; Supply, P.; Zanden, A.G. van der

    2010-01-01

    Five Mycobacterium tuberculosis isolates were obtained from three body sites from a Dutch patient. The isolates displayed a single genotype by 24-locus MIRU-VNTR typing (except for a single locus not amplified from one isolate) but were differentiated by small variations in IS6110 fingerprints,

  18. Structural studies on Mycobacterium tuberculosis RecA

    Indian Academy of Sciences (India)

    Structures of crystals of Mycobacterium tuberculosis RecA, grown and analysed under different conditions, provide insights into hitherto underappreciated details of molecular structure and plasticity. In particular, they yield information on the invariant and variable features of the geometry of the P-loop, whose binding to ATP ...

  19. The Use Of Rap-PCR In Studying Mycobacterium tuberculosis ...

    African Journals Online (AJOL)

    Mycobacterium tuberculosis is the second leading cause of death from infectious agent. This study sought to detect M. tuberculosis genes, which were specifically expressed, or upregulated during intracellular infection of. J774 murine macrophages; as such genes may be potential targets for novel drug action. J774 murine ...

  20. Construction of an internal amplification control for Mycobacterium ...

    African Journals Online (AJOL)

    Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) which mostly affects the lungs. The disease causes deaths of many people every year. There are different methods to detect MTB such as skin test, staining, culture and molecular techniques. Polymerase chain reaction (PCR) is a ...

  1. Modern lineages of Mycobacterium tuberculosis in Addis Ababa ...

    African Journals Online (AJOL)

    Background: The genotyping of Mycobacterium tuberculosis strains is important to have unique insights into the dissemination dynamics and evolutionary genetics of this pathogen and for TB control as it allows the detection of suspected outbreaks and the tracing of transmission chains. Objective: To characterize M.

  2. Beijing/W genotype Mycobacterium tuberculosis and drug resistance.

    NARCIS (Netherlands)

    Glynn, Judith R; Kremer, Kristin; Borgdorff, Martien W; Rodriguez, Mar Pujades; Soolingen, Dick van

    2006-01-01

    Beijing/W genotype Mycobacterium tuberculosis is widespread, may be increasing, and may have a predilection for drug resistance. Individual-level data on >29,000 patients from 49 studies in 35 countries were combined to assess the Beijing genotype's prevalence worldwide, trends over time and with

  3. Disseminated Mycobacterium avium complex in an immunocompetent host

    Directory of Open Access Journals (Sweden)

    Joseph M Yabes

    2017-01-01

    Full Text Available Disseminated Mycobacterium avium complex (DMAC has historically been described in the immunocompromised. The current epidemiologic research suggests that the incidence of nontuberculous mycobacterial infections is increasing. We present a case of DMAC infection manifesting as hepatic granulomas in a 35-year-old immunocompetent female. This case suggests DMAC infection in a patient without traditional epidemiological risk factors.

  4. The transmission of Mycobacterium tuberculosis in high burden settings

    NARCIS (Netherlands)

    Yates, Tom A.; Khan, Palwasha Y.; Knight, Gwenan M.; Taylor, Jonathon G.; McHugh, Timothy D.; Lipman, Marc; White, Richard G.; Cohen, Ted; Cobelens, Frank G.; Wood, Robin; Moore, David A. J.; Abubakar, Ibrahim

    2016-01-01

    Unacceptable levels of Mycobacterium tuberculosis transmission are noted in high burden settings and a renewed focus on reducing person-to-person transmission in these communities is needed. We review recent developments in the understanding of airborne transmission. We outline approaches to measure

  5. Cloning and expression of mce1A gene from Mycobacterium ...

    African Journals Online (AJOL)

    Background: Tuberculosis remains the leading cause of death in the world, especially wherever poverty, malnutrition and poor housing prevail. Mycobacterium tuberculosis Beijing strain is the most common strain that causes tuberculosis in Indonesia. The wide spread of tuberculosis has been further aggravated by ...

  6. Identification of Mycobacterium avium subsp. hominissuis Isolated From Drinking Water

    Science.gov (United States)

    Mycobacterium avium (MA) is divided into four subspecies based primarily on host-range and consists of MA subsp. avium (birds), MA subsp. silvaticum (wood pigeons), MA subsp. paratuberculosis (broad, poorly-defined host range), and the recently described MA subsp. hominissuis (hu...

  7. Prevalence of Mycobacterium bovis in Cattle Slaughtered at Sokoto ...

    African Journals Online (AJOL)

    This study was undertaken to screen cattle slaughtered at the Sokoto Central Abattoir for antibodies against Mycobacterium bovis. By the lateral flow technique (immunochromatography), using monoclonal antibodies for M. bovis (BioNote, Inc. Gyeonggi-do, Korea) and by post mortem examination. A total of 194 slaughtered ...

  8. Advances in the Laboratory Diagnosis of Mycobacterium Tuberculosis

    African Journals Online (AJOL)

    Mycobacterium tuberculosis (MTB), the agent of human tuberculosis remains a leading cause of mortality globally. Its resurgence during the last two decades is a reflection of its opportunistic relationship with HIV. The challenges associated with the disease are enormous and often debilitating. The role of clinical and ...

  9. Adaptation and evolution of drug-resistant Mycobacterium tuberculosis

    NARCIS (Netherlands)

    Bergval, I.L.

    2013-01-01

    Many studies have been conducted on drug resistance and the evolution of Mycobacterium tuberculosis. Notwithstanding, many molecular mechanisms facilitating the emergence, adaptation and spread of drug-resistant tuberculosis have yet to be discovered. This thesis reports studies of the adaptive

  10. BACTEC MGIT 960 TM system for screening of Mycobacterium ...

    African Journals Online (AJOL)

    This study was aimed to evaluate the recent technique (BACTEC MGIT 960 TM system) for screening of Mycobacterium tuberculosis complex among cattle in Egypt. From the 1180 cattle examined in three different Governorates (El-Sharkia, El-Gharbia and El-Monefeia) by single intradermal tuberculin test, 29 animals ...

  11. Degradation of morpholine by Mycobacterium sp. isolated from ...

    African Journals Online (AJOL)

    The biodegradation of morpholine has attracted much interest because morpholine causes environmental pollution. Ten species belonging to nine genera were tested for their abilities to degrade morpholine in mineral salts medium containing morpholine (1 g/l). Mycobacterium sp. isolated from polluted water sample ...

  12. Acanthamoeba Sp. S-11 phagocytotic activity on Mycobacterium ...

    African Journals Online (AJOL)

    Background: Mycobacterium leprae (M. leprae) is a pathogenic bacterium that causes leprosy. The presence of M. leprae in the environment is supported by microorganisms that act as the new host for M. leprae. Acanthamoeba's potential to be a host of M. leprae in the environment. Acanthamoeba sp. is Free Living ...

  13. Transmissie van Mycobacterium bovis tussen mens en dier

    NARCIS (Netherlands)

    Vries, de G.; Beer, de J.; Bakker, D.; Soolingen, D.

    2015-01-01

    Nederland is officieel vrij van rundertuberculose. Toch komt af en toe nog Mycobacterium bovis-tuberculose voor bij relatief jonge autochtone Nederlanders. Ook zijn er recent nog wel boviene-uitbraken geweest. Dat roept de vraag op of er ook nu nog transmissie is van M.bovis tussen mens en dier.

  14. MYCOBACTERIUM AVIUM AND DRINKING WATER WHAT ARE THE CONNECTIONS?

    Science.gov (United States)

    Background: Human Mycobacterium avium infections are only known to be acquired from environmental sources such as water and soil. We compared M. avium isolates from clinical and drinking water sources using molecular tools. Methods: M. avium was isolated from water samples colle...

  15. Tuberkulose forårsaget af Mycobacterium africanum

    DEFF Research Database (Denmark)

    Bek, Dorte; Kjeldsen, Marianne Kirstine; Hansen, Nikolaj Friis

    2010-01-01

    Tuberkulose (TB) forårsages af patogene arter fra Mycobacterium tuberculosis komplekset (MTBC) og har en incidens på cirka 7/100.000 i Danmark. På mistanke om TB hos en akut indlagt 40 årig afrikansk mand initieredes anti-TB behandling. Efter 13 timers indlæggelse afgik patienten ved døden. Fra...

  16. Host immunity to Mycobacterium tuberculosis and risk of tuberculosis

    DEFF Research Database (Denmark)

    Michelsen, Sascha Wilk; Soborg, Bolette; Agger, Else-Marie

    2016-01-01

    BACKGROUND: Human immune responses to latent Mycobacterium tuberculosis (Mtb) infection (LTBI) may enable individuals to control Mtb infection and halt progression to tuberculosis (TB), a hypothesis applied in several novel TB vaccines. We aimed to evaluate whether immune responses to selected LTBI...

  17. Benzothiazinones kill Mycobacterium tuberculosis by blocking arabinan synthesis

    DEFF Research Database (Denmark)

    Makarov, Vadim; Manina, Giulia; Mikusova, Katarina

    2009-01-01

    New drugs are required to counter the tuberculosis (TB) pandemic. Here, we describe the synthesis and characterization of 1,3-benzothiazin-4-ones (BTZs), a new class of antimycobacterial agents that kill Mycobacterium tuberculosis in vitro, ex vivo, and in mouse models of TB. Using genetics...

  18. A case of Manila type Mycobacterium tuberculosis infection in Japan

    Science.gov (United States)

    Usami, Osamu; Nakajima, Chie; Endo, Shiro; Inomata, Shinya; Kanamori, Hajime; Hirakata, Yoichi; Uchiyama, Bine; Kaku, Mitsuo; Suzuki, Yasuhiko; Hattori, Toshio

    2015-01-01

    Key Clinical Message A 76-year-old Japanese woman contracted a Mycobacterium tuberculosis (TB, Manila type) infection in Japan, despite never having traveled. However, her son was treated for TB in the Philippines 3 years before he stayed at her house. Spoligotyping allows us to identify the TB genotype and identify the route of infection. PMID:26273455

  19. Evaluación de rutas alternativas de síntesis de IAA en el complejo Pseudomonas syringae.

    OpenAIRE

    Pintado, Adrián; Pérez-Martínez, Isabel; Ramos, Cayo

    2016-01-01

    El ácido indol-3-acético (IAA) es una fitohormona perteneciente al grupo de las auxinas cuya producción está ampliamente distribuida entre bacterias asociadas a plantas. El IAA está implicado, entre otros procesos, en proliferación celular y maduración de las plantas. Además, se ha descrito el papel de esta hormona en la regulación de la expresión génica en bacterias. En bacterias fitopatógenas, se han descrito varias rutas de síntesis de IAA, siendo la mejor caracterizada la r...

  20. Simulador de un optimizador físico de consultas relacionales basado en costes que considere predicados complejos

    OpenAIRE

    Fernández Rodríguez, Sergio

    2012-01-01

    Una de las fases del proceso de tratamiento de una consulta que lleva a cabo el tipo de lenguaje utilizado por los SGBD (lenguajes declarativos) y, más concretamente en la fase de optimización, es la que da sentido al software sobre el cual se trabajará a lo largo de este proyecto. Hay tres tipos de optimización (semántica, sintáctica, física); dicho programa simula sólo uno de los tres tipos de optimizaciones que se realizan: la optimización física.

  1. Detection of Mycobacterium chelonae, Mycobacterium abscessus Group, and Mycobacterium fortuitum Complex by a Multiplex Real-Time PCR Directly from Clinical Samples Using the BD MAX System.

    Science.gov (United States)

    Rocchetti, Talita T; Silbert, Suzane; Gostnell, Alicia; Kubasek, Carly; Campos Pignatari, Antonio C; Widen, Raymond

    2017-03-01

    A new multiplex PCR test was designed to detect Mycobacterium chelonae, Mycobacterium abscessus group, and Mycobacterium fortuitum complex on the BD MAX System. A total of 197 clinical samples previously submitted for mycobacterial culture were tested using the new protocol. Samples were first treated with proteinase K, and then each sample was inoculated into the BD MAX Sample Buffer Tube. Extraction and multiplex PCR were performed by the BD MAX System, using the BD MAX ExK TNA-3 extraction kit and BD TNA Master Mix, along with specific in-house designed primers and probes for each target. The limit of detection of each target, as well as specificity, was evaluated. Of 197 clinical samples included in this study, 133 were positive and 60 were negative for mycobacteria by culture, and another 4 negative samples were spiked with M. chelonae ATCC 35752. The new multiplex PCR on the BD MAX had 97% concordant results with culture for M. abscessus group detection, 99% for M. chelonae, and 100% for M. fortuitum complex. The new multiplex PCR test performed on the BD MAX System proved to be a sensitive and specific test to detect M. chelonae, M. abscessus group, and M. fortuitum complex by real-time PCR on an automated sample-in results-out platform. Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

  2. Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatis.

    Science.gov (United States)

    Salazar, L; Fsihi, H; de Rossi, E; Riccardi, G; Rios, C; Cole, S T; Takiff, H E

    1996-04-01

    The genus Mycobacterium is composed of species with widely differing growth rates ranging from approximately three hours in Mycobacterium smegmatis to two weeks in Mycobacterium leprae. As DNA replication is coupled to cell duplication, it may be regulated by common mechanisms. The chromosomal regions surrounding the origins of DNA replication from M. smegmatis, M. tuberculosis, and M. leprae have been sequenced, and show very few differences. The gene order, rnpA-rpmH-dnaA-dnaN-recF-orf-gyrB-gyrA, is the same as in other Gram-positive organisms. Although the general organization in M. smegmatis is very similar to that of Streptomyces spp., a closely related genus, M. tuberculosis and M. leprae differ as they lack an open reading frame, between dnaN and recF, which is similar to the gnd gene of Escherichia coli. Within the three mycobacterial species, there is extensive sequence conservation in the intergenic regions flanking dnaA, but more variation from the consensus DnaA box sequence was seen than in other bacteria. By means of subcloning experiments, the putative chromosomal origin of replication of M. smegmatis, containing the dnaA-dnaN region, was shown to promote autonomous replication in M. smegmatis, unlike the corresponding regions from M. tuberculosis or M. leprae.

  3. Infections with Mycobacterium tuberculosis and Mycobacterium avium among HIV-infected patients after the introduction of highly active antiretroviral therapy. EuroSIDA Study Group JD

    DEFF Research Database (Denmark)

    Kirk, O; Gatell, J M; Mocroft, A

    2000-01-01

    the introduction of HAART, using data from the EuroSIDA study, a European, multicenter observational cohort of more than 7,000 patients. Overall incidences of Mycobacterium tuberculosis (TB) and Mycobacterium avium complex (MAC) were 0.8 and 1.4 cases/100 person-years of follow-up (PYF), decreasing from 1.8 (TB...

  4. Detection of Mycobacterium bovis and Mycobacterium tuberculosis from Cattle: Possible Public Health Relevance

    DEFF Research Database (Denmark)

    Thakur, Aneesh; Sharma, Mandeep; Katoch, Vipin C.

    2012-01-01

    Mycobacterium bovis and Mycobacterium tuberculosis infect both animals and humans. The disease epidemiology by these agents differs in developed and developing countries due to the differences in the implementation of the prevention and control strategies. The present study describes the detectio...

  5. Comparative Genomics and Proteomic Analysis of Four Non-tuberculous Mycobacterium Species and Mycobacterium tuberculosis Complex : Occurrence of Shared Immunogenic Proteins

    NARCIS (Netherlands)

    Gcebe, Nomakorinte; Michel, Anita; Gey van Pittius, Nicolaas C; Rutten, Victor

    2016-01-01

    The Esx and PE/PPE families of proteins are among the most immunodominant mycobacterial antigens and have thus been the focus of research to develop vaccines and immunological tests for diagnosis of bovine and human tuberculosis, mainly caused by Mycobacterium bovis and Mycobacterium tuberculosis,

  6. Mycobacterium malmesburyense sp. nov., a non-tuberculous species of the genus Mycobacterium revealed by multiple gene sequence characterization.

    Science.gov (United States)

    Gcebe, Nomakorinte; Rutten, Victor; Pittius, Nicolaas Gey van; Naicker, Brendon; Michel, Anita

    2017-04-01

    Non-tuberculous mycobacteria (NTM) are ubiquitous in the environment, and an increasing number of NTM species have been isolated and characterized from both humans and animals, highlighting the zoonotic potential of these bacteria. Host exposure to NTM may impact on cross-reactive immune responsiveness, which may affect diagnosis of bovine tuberculosis and may also play a role in the variability of the efficacy of Mycobacterium bovis BCG vaccination against tuberculosis. In this study we characterized 10 NTM isolates originating from water, soil, nasal swabs of cattle and African buffalo as well as bovine tissue samples. These isolates were previously identified during an NTM survey and were all found, using 16S rRNA gene sequence analysis to be closely related to Mycobacterium moriokaense. A polyphasic approach that included phenotypic characterization, antibiotic susceptibility profiling, mycolic acid profiling and phylogenetic analysis of four gene loci, 16S rRNA, hsp65, sodA and rpoB, was employed to characterize these isolates. Sequence data analysis of the four gene loci revealed that these isolates belong to a unique species of the genus Mycobacterium. This evidence was further supported by several differences in phenotypic characteristics between the isolates and the closely related species. We propose the name Mycobacterium malmesburyense sp. nov. for this novel species. The type strain is WCM 7299T (=ATCC BAA-2759T=CIP 110822T).

  7. Mycobacterium angelicum sp. nov., a non-chromogenic, slow-growing species isolated from fish and related to Mycobacterium szulgai.

    Science.gov (United States)

    Pourahmad, Fazel; Pate, Mateja; Ocepek, Matjaž; Borroni, Emanuele; Cabibbe, Andrea M; Capitolo, Eleonora; Cittaro, Davide; Frizzera, Eliana; Jenčič, Vlasta; Mariottini, Alessandro; Marumo, Kenji; Vaggelli, Guendalina; Cirillo, Daniela M; Tortoli, Enrico

    2015-12-01

    The name 'Mycobacterium angelicum' dates back to 2003 when it was suggested for a slowly growing mycobacterium isolated from freshwater angelfish. This name is revived here and the novel species is proposed on the basis of the polyphasic characterization of four strains including the original one. The four strains presented 100 % 16S rRNA gene sequence similarity with Mycobacterium szulgai but clearly differed from M. szulgai for the milky white aspect of the colonies. The sequence similarity with the type strain of M. szulgai ranged, in eight additionally investigated genetic targets, from 78.9 to 94.3 %, an evident contrast with the close relatedness that emerged at the level of 16S rRNA gene. The average nucleotide identity between the genomes of M. szulgai DSM 44166T and strain 126/5/03T (type strain of the novel species) was 92.92 %, and supported the status of independent species. The confirmation of the name Mycobacterium angelicum sp. nov. is proposed, with strain 126/5/03T ( = CIP 109313T = DSM 45057T) as the type strain.

  8. Line probe assay for differentiation within Mycobacterium tuberculosis complex. Evaluation on clinical specimens and isolates including Mycobacterium pinnipedii

    DEFF Research Database (Denmark)

    Kjeldsen, Marianne Kirstine; Bek, Dorte; Rasmussen, Erik Michael

    2009-01-01

    A line probe assay (GenoType MTBC) was evaluated for species differentiation within the Mycobacterium tuberculosis complex (MTBC). We included 387 MTBC isolates, 43 IS6110 low-copy MTBC isolates, 28 clinical specimens with varying microscopy grade, and 30 isolates of non-tuberculous mycobacteria...

  9. Contribución del análisis RFLP del IS6110 de Mycobacterium tuberculosis al diseño y refinamiento de estrategias para el control de la tuberculosis en Colombia Contribution of M. tuberculosis IS6110 based RFLP assay to the design and refinement of tuberculosis control strategies in Colombia

    Directory of Open Access Journals (Sweden)

    Doris Amanda Rosero

    2008-09-01

    Full Text Available Una alternativa de mejoramiento de las estrategias para el control de la tuberculosis, la ofrecen los métodos para la diferenciación de cepas de M. tuberculosis. La literatura evidencia que la técnica RFLP del segmento de inserción IS6110 está ampliamente estandarizada a nivel internacional y ha demostrado ser un buen instrumento para orientar estrategias locales de control. Mediante una revisión exhaustiva de la literatura, el presente estudio pretende establecer si esta técnica molecular puede contribuir al diseño y refinamiento de estrategias para el control de la tuberculosis en Colombia. En esta revisión se analizaron los resultados de los estudios publicados entre 1993 y 2008, realizados con la técnica en países en desarrollo, incluida Colombia. Los resultados sugieren que en el contexto colombiano esta técnica puede ofrecer información útil para los directores del programa de control de tuberculosis y, por tanto, debe seguir siendo realizada. Para establecer la periodicidad, las poblaciones blanco y otras condiciones óptimas para la realización de la técnica, se necesitan estudios de investigación operativa que incluyan análisis de costo-efectividad y costo-utilidad.Molecular biology methods offer an alternative for improving tuberculosis control strategies through M. tuberculosis strain typing techniques. The international literature shows that RFLP of the insertion element IS6110 is widely standardized internationally and has proved to be a useful tool to guide local tuberculosis control strategies. By means of a thorough literature review, this study aimed to determine if this molecular based method could be useful for the design and refinement of tuberculosis control strategies in Colombia. Results from epidemiologic studies published between 1995 and 2008 which used this technique in developing countries, including Colombia, were analyzed. Our results suggest that in the Colombian context this molecular technique

  10. Feline leprosy due to Mycobacterium lepraemurium.

    Science.gov (United States)

    O'Brien, Carolyn R; Malik, Richard; Globan, Maria; Reppas, George; McCowan, Christina; Fyfe, Janet A

    2017-07-01

    This paper, the second in a series of three on 'feline leprosy', provides a detailed description of disease referable to Mycobacterium lepraemurium, the most common cause of feline leprosy worldwide. Cases were sourced retrospectively and prospectively for this observational study, describing clinical, geographical and molecular microbiological data for cats definitively diagnosed with M lepraemurium infection. A total of 145 cases of feline leprosy were scrutinised; 114 'new' cases were sourced from the Victorian Infectious Diseases Reference Laboratory records, veterinary pathology laboratories or veterinarians, and 31 cases were derived from six published studies. Sixty-five cats were definitively diagnosed with M lepraemurium infection. Typically, cats were 1-3 years of age when first infected, with a male gender predilection. Affected cats were generally systemically well. All had outdoor access. Lesions tended to consist of one or more cutaneous/subcutaneous nodules, typically located on the head and/or forelimbs, possibly reflecting the most likely locations for a rodent bite as the site of inoculation for organisms. Nodules had the propensity to ulcerate at some stage in the clinical course. The cytological and histological picture varied from tuberculoid, with relatively low bacterial numbers, to lepromatous with moderate to high bacterial numbers. Treatment was varied, although most cats underwent surgical resection of lesions with adjunctive medical therapy, most often using a combination of oral clarithromycin and rifampicin. Prognosis for recovery was generally good, and in two cases there was spontaneous remission without the requirement for medical intervention. Untreated cats continued to enjoy an acceptable quality of life despite persistence of the disease, which extended locally but had no apparent tendency to disseminate to internal organs. M lepraemurium causes high bacterial index (lepromatous) or low bacterial index (tuberculoid) feline

  11. Revival and emended description of 'Mycobacterium paraffinicum' Davis, Chase and Raymond 1956 as Mycobacterium paraffinicum sp. nov., nom. rev.

    Science.gov (United States)

    Toney, Nadege; Adekambi, Toidi; Toney, Sean; Yakrus, Mitchell; Butler, W Ray

    2010-10-01

    The omission of the name 'Mycobacterium paraffinicum' from the Approved Lists of Bacterial Names was due to phenotypic confusion surrounding a close relationship with Mycobacterium scrofulaceum. Correspondingly, 'M. paraffinicum' strains grew slowly in > 7 days, stained acid-alcohol-fast and produced yellow-pigmented, smooth, waxy colonies in the dark at an optimal temperature of 35°C. However, 'M. paraffinicum' strains demonstrated no activity for urease, nicotinamidase or pyrazinamidase and lacked growth at 42°C, unlike M. scrofulaceum. The mycolic acid pattern, as determined by HPLC, clustered 'M. paraffinicum' with M. scrofulaceum, Mycobacterium avium and Mycobacterium parascrofulaceum. Strains were fully susceptible to linezolid, rifabutin, clarithromycin and amikacin. Examination of the historical reference strain of 'M. paraffinicum', ATCC 12670, and five additional isolates using comparative studies with 16S rRNA, hsp65 and rpoB gene and concatenated sequences showed that they formed a tight taxonomic group that was distinct from similar non-tuberculous mycobacteria. Multilocus enzyme electrophoresis (MEE) analysis confirmed a close association of the five additional isolates with the reference strain of 'M. paraffinicum' with a genetic distance of 0.12 and showed that all six strains were distinct from other closely related species. These genetic results provided unambiguous evidence of the uniqueness of this slowly growing, scotochromogenic species and supported the revival of the name as Mycobacterium paraffinicum (ex Davis, Chase and Raymond 1956) sp. nov., nom. rev. We propose the previously deposited reference strain ATCC 12670(T) =DSM 44181(T) =NCIMB 10420(T), located in collections worldwide, as the type strain.

  12. Assessing the inactivation of Mycobacterium avium subsp. paratuberculosis during composting of livestock carcasses.

    Science.gov (United States)

    Tkachuk, Victoria L; Krause, Denis O; McAllister, Tim A; Buckley, Katherine E; Reuter, Tim; Hendrick, Steve; Ominski, Kim H

    2013-05-01

    Mycobacterium avium subsp. paratuberculosis causes Johne's disease (JD) in ruminants, with substantial economic impacts on the cattle industry. Johne's disease is known for its long latency period, and difficulties in diagnosis are due to insensitivities of current detection methods. Eradication is challenging as M. avium subsp. paratuberculosis can survive for extended periods within the environment, resulting in new infections in naïve animals (W. Xu et al., J. Environ. Qual. 38:437-450, 2009). This study explored the use of a biosecure, static composting structure to inactivate M. avium subsp. paratuberculosis. Mycobacterium smegmatis was also assessed as a surrogate for M. avium subsp. paratuberculosis. Two structures were constructed to hold three cattle carcasses each. Naturally infected tissues and ground beef inoculated with laboratory-cultured M. avium subsp. paratuberculosis and M. smegmatis were placed in nylon and plastic bags to determine effects of temperature and compost environment on viability over 250 days. After removal, samples were cultured and growth of both organisms was assessed after 12 weeks. After 250 days, M. avium subsp. paratuberculosis was still detectable by PCR, while M. smegmatis was not detected after 67 days of composting. Furthermore, M. avium subsp. paratuberculosis remained viable in both implanted nylon and plastic bags over the composting period. As the compost never reached a homogenous thermophilic (55 to 65°C) state throughout each structure, an in vitro experiment was conducted to examine viability of M. avium subsp. paratuberculosis after exposure to 80°C for 90 days. Naturally infected lymph tissues were mixed with and without compost. After 90 days, M. avium subsp. paratuberculosis remained viable despite exposure to temperatures typically higher than that achieved in compost. In conclusion, it is unlikely composting can be used as a means of inactivating M. avium subsp. paratuberculosis associated with cattle

  13. Assessing the Inactivation of Mycobacterium avium subsp. paratuberculosis during Composting of Livestock Carcasses

    Science.gov (United States)

    Tkachuk, Victoria L.; Krause, Denis O.; McAllister, Tim A.; Buckley, Katherine E.; Reuter, Tim; Hendrick, Steve

    2013-01-01

    Mycobacterium avium subsp. paratuberculosis causes Johne's disease (JD) in ruminants, with substantial economic impacts on the cattle industry. Johne's disease is known for its long latency period, and difficulties in diagnosis are due to insensitivities of current detection methods. Eradication is challenging as M. avium subsp. paratuberculosis can survive for extended periods within the environment, resulting in new infections in naïve animals (W. Xu et al., J. Environ. Qual. 38:437-450, 2009). This study explored the use of a biosecure, static composting structure to inactivate M. avium subsp. paratuberculosis. Mycobacterium smegmatis was also assessed as a surrogate for M. avium subsp. paratuberculosis. Two structures were constructed to hold three cattle carcasses each. Naturally infected tissues and ground beef inoculated with laboratory-cultured M. avium subsp. paratuberculosis and M. smegmatis were placed in nylon and plastic bags to determine effects of temperature and compost environment on viability over 250 days. After removal, samples were cultured and growth of both organisms was assessed after 12 weeks. After 250 days, M. avium subsp. paratuberculosis was still detectable by PCR, while M. smegmatis was not detected after 67 days of composting. Furthermore, M. avium subsp. paratuberculosis remained viable in both implanted nylon and plastic bags over the composting period. As the compost never reached a homogenous thermophilic (55 to 65°C) state throughout each structure, an in vitro experiment was conducted to examine viability of M. avium subsp. paratuberculosis after exposure to 80°C for 90 days. Naturally infected lymph tissues were mixed with and without compost. After 90 days, M. avium subsp. paratuberculosis remained viable despite exposure to temperatures typically higher than that achieved in compost. In conclusion, it is unlikely composting can be used as a means of inactivating M. avium subsp. paratuberculosis associated with cattle

  14. Organización del trabajo y dispositivos de control en el sector automotriz: el toyotismo como sistema complejo de racionalización Labour Structure and Control Devices in the Automotive Industry: The "toyotism" as a complex rationalization system

    Directory of Open Access Journals (Sweden)

    Diego Alvarez Newman

    2012-06-01

    Full Text Available El presente artículo pretende problematizar la concepción toyotista a partir de la descripción y el análisis del Sistema de Producción Toyota (SPT, sus principios y estrategias de aplicación. Entendemos al SPT como un sistema complejo de racionalización de la producción y de los trabajadores que opera mediante la aplicación de variados dispositivos de control técnico y social. El SPT postula ciertos principios básicos que apuntan a la mejora continua de la productividad y la calidad, poniendo en juego un andamiaje estratégico para intervenir sobre el control del tiempo y del espacio en la fábrica y sobre el control de los ritmos, movimientos y valores de los trabajadores. Estos dispositivos no operan solo como reguladores de la producción y la productividad sino que además operan como dispositivos de flexibilización de la fuerza de trabajo y dispositivos de cooptación sindical para minimizar la conflictividad laboral. Formar para contar con trabajadores involucrados con los objetivos de la empresa y con un sindicato que adhiera a la filosofía Toyota constituye un fuerte factor de productividad en el sentido en que no se verá afectado el circuito de la mejora continua.The following article intends to examine the concept of "toyotism" based on the description and analysis of the Toyota Production System (TPS and its´ principles and strategies application. We infer the TPS as a complex system which rationalizes both the production and the workers. This system works through the implementation of diverse technical and social devices. The TPS postulates certain basic principles aiming a continuous productivity and quality improvement, applying a strategic configuration to intervene in the control of the factory´s time and space as well as in the regulation of rhythms, movements and worker´s values. These devices do not only operate as production and productivity watchers, but also function as workforce flexibilization and

  15. Análisis de sistema de potencia desbalanceado con el empleo de herramientas estadísticas, coeficientes complejos y redes neuronales artificiales; Statistical, Complex Factor and Neural Networks Analysis and Simulation of an Unbalance Power Systems

    Directory of Open Access Journals (Sweden)

    Marcos A de Armas Teyra

    2011-02-01

    Full Text Available Se caracteriza estadísticamente el desbalance de tensión en un sistema eléctrico de potencia en términos de laIEEE y se señalan algunas informaciones útiles adicionales que se obtienen de esta. Se muestra cuán sostenidapuede ser la explotación de un conjunto de potentes motores asincrónicos fuera de las normas internacionaleshaciéndose énfasis en aspectos energéticos al determinar la potencia demandada y la energía adicional consumidadebido a la asimetría. Por último, se presenta una red neuronal cuya función es determinar el desbalance, emitirseñales de alarma y producir el disparo del sistema si fuese necesario. Para este propósito se utilizan no solo lastensiones de fase, sino las corrientes de línea y los coeficientes complejos de desbalance. Se concluye con queel desbalance es un problema energético que produce demandas y pérdidas que pueden ser cuantificadas por elcoeficiente de asimetría y el análisis estadístico. Las redes neuronales pueden ser entrenadas para ofreceralarma, protección y otras tareas en los sistemas eléctricos de potencia. También pueden ser incorporadas a lossistemas modernos de mediciones digitalizadas en los servicios industriales. Para la validación de los resultados,se emplean las mediciones recién efectuadas en una estación de bombeo del acueducto de la provincia deCienfuegos, Cuba.  There are tree goals in this article; statistical characterization of an unbalanced power system, show how longan asynchronous group of motors can be exploited out of international unbalance standards focused on powerdemand and energy consumption due to asymmetry complex defined factor and to present an artificial neuralnetwork used to send warning signals or to protect the motors under abnormal unbalanced conditions. As a case,a water supply station in Cienfuegos`s province is presented.sequence and the possibilities that this designpresents for the increase of the security of the installationI.

  16. El complejo manufacturero de la Real Fábrica de Sedas de Talavera de la Reina (1785

    Directory of Open Access Journals (Sweden)

    Luis Francisco Peñalver Ramos

    1996-01-01

    Full Text Available La «Real Fábrica de Tejidos de Seda, Plata y Oro» de Talavera de la Reina se fundó a mediados del siglo xviii bajo los auspicios del rey Fernando VI y teniendo como promotor principal a su Secretario de Estado, José de Carvajal y Lancaster. Su creación constituye un exponente más de la política económica de los Borbones españoles, iniciada por Felipe V tras la Guerra de Sucesión mediante un programa de reformas económicas con el fin de alcanzar una balanza comercial favorable para nuestra nación, y evitar de esta forma la dependencia del exterior, especialmente de Francia e Inglaterra. Para lograr llevar a cabo este proyecto era necesario una puesta al día de la industria, y entre otros sectores, del textil.

  17. Hydrocarbon reserves, energy assurance and macroeconomics: a complex balance; Reservas de hidrocarburos, seguridad energetica y macroeconomia: un balance complejo

    Energy Technology Data Exchange (ETDEWEB)

    Alarco Tosoni, German [TYH Economia, S.A. de C.V., Mexico, D.F.(Mexico)

    2006-11-15

    The available public information relative to the limited discovered and undiscovered crude and natural gas reserves in Mexico, from an international comparison perspective, is analyzed. Through the evaluation of the national energy programs, a limited conception of energy security is made clear concerning that posed by the other countries within North America. Various scenarios are stated regarding proved/production reserves to 2020 which will induce, together with the notion of energy security, a reduction in the current production levels - crude oil exports. The paradox referring to the fact that given certain reduction levels in the exports' crude platform, both the product level as well as real public expenditure will rise, is discovered. Finally, the macroeconomic effects of the proposal and of the necessary complementary policies needed to be designed and implemented in order to improve our macroeconomic performance are also evaluated. [Spanish] Se analiza la informacion publica disponible sobre las reservas limitadas de crudo y gas natural descubiertas y no descubiertas de Mexico en una perspectiva internacional comparada. Al evaluar los programas energeticos nacionales se transparenta una concepcion limitada de la seguridad energetica, respecto de lo planteado por los otros paises de America del Norte. Se plantean diferentes escenarios de reservas probadas/produccion al 2020 que inducirian, conjuntamente con la incorporacion de la nocion de seguridad energetica, a reducir los niveles actuales de produccion - exportacion de crudo. Se descubre la paradoja de que para determinados niveles de reduccion de la plataforma de exportacion de crudo tanto el nivel de producto como de gasto publico real aumentan. Por ultimo, se evaluan tanto los efectos macroeconomicos de la propuesta como de algunas politicas complementarias que seria necesario disenar e implantar para mejorar nuestro desempeno macroeconomico.

  18. Real-Time Measurement of Host Bioenergetics During Mycobacterium Tuberculosis Infection

    Science.gov (United States)

    2015-05-01

    AWARD NUMBER: W81XWH-13-1-0149 TITLE: “Real-Time Measurement of Host Bioenergetics During Mycobacterium Tuberculosis Infection...successfully adapted metabolic flux analysis using a Seahorse XF96 metabolic flux analyzer to study Mycobacterium tuberculosis energy metabolism in an...Mycobacterium tuberculosis function. In: Systems Biology of Tuberculosis . Editors: J McFadden, D Beste and A Kierzek. 2013. Springer, New York, NY. 2

  19. Methylobacterium spp. as an indicator for the presence or absence of Mycobacterium spp.

    OpenAIRE

    Falkinham III, Joseph O.; Williams, Myra D.; Kwait, Rebecca; Lande, Leah

    2016-01-01

    Objective/Background: A published survey of bacteria in showerhead biofilm samples revealed that Methylobacterium spp. and Mycobacterium spp. seldom coexisted in biofilms. Method: To confirm that information, biofilm samples were collected from household plumbing of Mycobacterium avium patients and Methylobacterium spp. and M. avium numbers were measured by direct colony counts. Results: The results demonstrated that if Methylobacterium spp. were present, Mycobacterium spp. were absent,...

  20. Cervical Lymphadenitis by Mycobacterium triplex in an Immunocompetent Child: Case Report and Review

    OpenAIRE

    Caruso, G.; Angotti, R.; Molinaro, F.; Benicchi, E.; Cerchia, E.; Messina, M.

    2013-01-01

    Mycobacterium triplex was first described in 1996. This nontuberculous Mycobacterium causes a severe pulmonary disease in immunocompromised patients but it can involve also healthy patients. A literature search was made on the PubMed database and it produced only few cases of children with cervical lymphadenitis due to this Mycobacterium Triplex. We are describing a case of M. triplex cervical lymphadenitis in an immunocompetent child.

  1. AL Amyloidosis

    Directory of Open Access Journals (Sweden)

    Desport Estelle

    2012-08-01

    Full Text Available Abstract Definition of the disease AL amyloidosis results from extra-cellular deposition of fibril-forming monoclonal immunoglobulin (Ig light chains (LC (most commonly of lambda isotype usually secreted by a small plasma cell clone. Most patients have evidence of isolated monoclonal gammopathy or smoldering myeloma, and the occurrence of AL amyloidosis in patients with symptomatic multiple myeloma or other B-cell lymphoproliferative disorders is unusual. The key event in the development of AL amyloidosis is the change in the secondary or tertiary structure of an abnormal monoclonal LC, which results in instable conformation. This conformational change is responsible for abnormal folding of the LC, rich in β leaves, which assemble into monomers that stack together to form amyloid fibrils. Epidemiology AL amyloidosis is the most common type of systemic amyloidois in developed countries with an estimated incidence of 9 cases/million inhabitant/year. The average age of diagnosed patients is 65 years and less than 10% of patients are under 50. Clinical description The clinical presentation is protean, because of the wide number of tissues or organs that may be affected. The most common presenting symptoms are asthenia and dyspnoea, which are poorly specific and may account for delayed diagnosis. Renal manifestations are the most frequent, affecting two thirds of patients at presentation. They are characterized by heavy proteinuria, with nephrotic syndrome and impaired renal function in half of the patients. Heart involvement, which is present at diagnosis in more than 50% of patients, leading to restrictive cardiopathy, is the most serious complication and engages prognosis. Diagnostic methods The diagnosis relies on pathological examination of an involved site showing Congo red-positive amyloid deposits, with typical apple-green birefringence under polarized light, that stain positive with an anti-LC antibody by immunohistochemistry and

  2. "Síndrome complejo de malnutrición e inflamación" en la hemodiálisis crónica

    Directory of Open Access Journals (Sweden)

    Pablo Young

    2011-02-01

    Full Text Available La malnutrición calórico-proteica y la inflamación suelen ser condiciones comunes y concurrentes en pacientes con hemodiálisis crónica, asociándose ambas a mal pronóstico. La hiporexia y el hipercatabolismo son características comunes y frecuentes. Se ha sugerido que la primera es secundaria a la inflamación. Si bien la evidencia no es concluyente, se ha acuñado el término síndrome complejo de malnutrición e inflamación para englobar esta situación clínica, independientemente de la causa originaria. Posibles causas de este síndrome incluyen diferentes comorbilidades, estrés oxidativo, pérdida de nutrientes a través de la diálisis, hiporexia, toxinas urémicas, elevación de citoquinas inflamatorias, sobrecarga de volumen, hiperfosfatemia, subdiálisis, entre otros. Se cree que en este síndrome la resistencia a la eritropoyetina, promueve la enfermedad aterosclerótica, disminuyendo la calidad de vida e incrementando el tiempo de internación y la mortalidad. Este síndrome origina un bajo índice de masa corporal, hipocolesterolemia, sarcopenia e hipocreatininemia, e hipohomocisteinemia, paradójicamente incrementando el riesgo cardiovascular. A este fenómeno se lo ha denominado "epidemiología reversa". Por lo tanto, y dentro de ciertos límites, la obesidad, la hipercolesterolemia, el incremento de la creatinina y de la homocisteína jugarían un rol protector, asociándose a mejor pronóstico. No existe consenso sobre cómo determinar la gravedad del síndrome complejo de malnutrición e inflamación, su abordaje y su tratamiento. En este trabajo se discuten varias herramientas diagnósticas y modalidades de tratamiento. El correcto manejo de este cuadro podría disminuir en última instancia la enfermedad cardiovascular, principal causa de óbito en esta población.

  3. Disseminated Infection by Mycobacterium sherrisii and Histoplasma capsulatum in an African HIV-Infected Patient

    Science.gov (United States)

    Taján, Juan; Espasa, Mateu; Sala, Montserrat; Navarro, Marta; Font, Bernat; González-Martín, Julián; Segura, Ferran

    2013-01-01

    Mycobacterium sherrisii is a new species of opportunistic, slow-growing, non-tuberculous Mycobacterium closely related to Mycobacterium simiae that can currently be identified with the sequence of 16S rARN gene and the heat-shock protein 65. Few cases of patients infected by this Mycobacterium have been reported and all of them were associated with human immunodeficiency virus or other immunosuppressive conditions. Clinical management is complex, because there is not a clear correlation between the in vitro antibiotic susceptibility testing and the patient's clinical outcome. PMID:23419367

  4. Una ventana al mundo de Kafka.

    Directory of Open Access Journals (Sweden)

    Guillermo Sánchez Medina

    2003-03-01

    Full Text Available

    COMENTARIO AL LIBRO " EL MUNDO PSICOLÓGICO DE KAFKA" POR EL ACADÉMICO ADOLFO DE FRANCISCO ZEA.

     

    Fui invitado en forma comedida, por el doctor Adolfo De Francisco, para comentar su obra: "El Mundo Psicológico de Kafka". Respondí que no era literato y menos crítico de Kafka. Sin embargo, acepté
    hacerlo siendo consciente de las limitaciones que me acompañan y para las que pido de todos ustedes su consideración. Tenía sí el deseo al reto de lo incógnito.
    Además sus argumentos fueron contundentes, pues se referían al compromiso con la Academia y, sin decírmelo, era también con él en nuestra historia compartida. Mis motivaciones y expectativas personales conscientes eran múltiples para sumergirme en sus ideas que mueven el espíritu desde la piel a lo trascendente, de lo interno a lo externo y viceversa, y en que cada palabra puede ser objeto de análisis profundo, pues esa es su esencia. Por ejemplo cuando el doctor De Francisco se refiere a la sublimación y a la estética, a la política, a la religiosidad, a la burguesía, al campesinado, a la nobleza, al absolutismo, al arte o a la historia, a la cosmovisión sociológica y literaria, al psicoanálisis para afrontar la realidad interna y externa, entonces el autor penetra en el mundo de la fantasía y del psiquismo, para entender el simbolismo implícito de Kafka.
    Las motivaciones personales inconscientes, pertenecen a las tendencias irracionales de hundirme en espacios complejos para luego intentar'ordenarlos; en medio está
    la contratransferencia que me une con el autor Adolfo De Francisco y otra la que se refiere a la temática kafkiana...

  5. Activity of Scottish plant, lichen and fungal endophyte extracts against Mycobacterium aurum and Mycobacterium tuberculosis.

    Science.gov (United States)

    Gordien, Andréa Y; Gray, Alexander I; Ingleby, Kevin; Franzblau, Scott G; Seidel, Véronique

    2010-05-01

    With tuberculosis the leading bacterial killer worldwide and other mycobacterial diseases on the increase, the search for new antimycobacterial agents is timely. In this study, extracts from plants, lichens and fungal endophytes of Scottish provenance were screened for activity against Mycobacterium aurum and M. tuberculosis H(37)Rv. The best activity against M. aurum was observed for extracts of Juniperus communis roots and Cladonia arbuscula (MIC = 4 microg/mL), and a fungal endophyte isolated from Vaccinium myrtillus (MIC = 8 microg/mL). The best activity against M. tuberculosis was observed for extracts of C. arbuscula, Empetrum nigrum, J. communis roots, Calluna vulgaris aerial parts, Myrica gale roots and stems (93 to 99% inhibition at 100 microg/mL). Potent antitubercular activity (90 to 96% inhibition at 100 microg/mL) was also observed for the ethanol extracts of Xerocomus badius, Chalciporus piperatus, Suillus luteus and of endophytes isolated from C. vulgaris, E. nigrum, Vaccinium vitis-idaea and V. myrtillus. The results obtained this study provide, in part, some scientific basis for the traditional use of some of the selected plants in the treatment of tuberculosis. They also indicate that fungal endophytes recovered from Scottish plants are a source of antimycobacterial agents worthy of further investigation. Copyright (c) 2009 John Wiley & Sons, Ltd.

  6. Mycobacterium fortuitum and Mycobacterium chelonae biofilm formation under high and low nutrient conditions.

    Science.gov (United States)

    Hall-Stoodley, L; Keevil, C W; Lappin-Scott, H M

    1998-12-01

    The rapidly growing mycobacteria (RGM) are broadly disbursed in the environment. They have been recovered from freshwater, seawater, wastewater and even potable water samples and are increasingly associated with non-tuberculous mycobacterial disease. There is scant evidence that non-tuberculous mycobacteria (NTM) and RGM form biofilms. Therefore, an experimental system was designed to assess the ability of RGM to form biofilms under controlled laboratory conditions. A flat plate reactor flow cell was attached to either a high or low nutrient reservoir and monitored by image analysis over time. Two surfaces were chosen for assessment of biofilm growth: silastic which is commonly used in medical settings and high density polyethylene (HDPE) which is prevalent in water distribution systems. The results show that Mycobacterium fortuitum and M. chelonae formed biofilms under both high and low nutrient conditions on both surfaces studied. These results suggest that RGM may form biofilms under a variety of conditions in industrial and medical environments. 1998 Society of Applied Microbiology.

  7. The Complete Structure of the Mycobacterium smegmatis 70S Ribosome

    Directory of Open Access Journals (Sweden)

    Jendrik Hentschel

    2017-07-01

    Full Text Available The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-Å cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics.

  8. Mycobacterium tuberculosis Complex Members Adapted to Wild and Domestic Animals.

    Science.gov (United States)

    Malone, Kerri M; Gordon, Stephen V

    2017-01-01

    The Mycobacterium tuberculosis complex (MTBC) is composed of several highly genetically related species that can be broadly classified into those that are human-host adapted and those that possess the ability to propagate and transmit in a variety of wild and domesticated animals. Since the initial description of the bovine tubercle bacillus, now known as Mycobacterium bovis, by Theobald Smith in the late 1800's, isolates originating from a wide range of animal hosts have been identified and characterized as M. microti, M. pinnipedii, the Dassie bacillus, M. mungi, M. caprae, M. orygis and M. suricattae. This chapter outlines the events resulting in the identification of each of these animal-adapted species, their close genetic relationships, and how genome-based phylogenetic analyses of species-specific variation amongst MTBC members is beginning to unravel the events that resulted in the evolution of the MTBC and the observed host tropism between the human- and animal-adapted member species.

  9. Adhesion of Mycobacterium smegmatis to Charged Surfaces and Diagnostics Implications

    Science.gov (United States)

    Gorse, Diane; Dhinojwala, Ali; Moore, Francisco

    Pulmonary tuberculosis (PTB) causes more than 1 million deaths annually. Smear microscopy is a primary rapid detection tool in areas where 95 % of PTB cases occur. This technique, in which the sputum of a symptomatic patient is stained and examined using a light microscope for Mycobacterium tuberculosis (MTB) shows sensitivity between 20 and 60 %. Insufficient bacterial isolation during sample preparation may be a reason for low sensitivity. We are optimizing a system to capture bacteria on the basis of electrostatic interactions to more thoroughly isolate bacteria from suspension and facilitate more accurate detection. Silica supports coated with positively-charged polyelectrolyte, poly(diallyldimethylammonium chloride), captured approximately 4.1 times more Mycobacterium smegmatis, a model organism for MTB, than was captured on negatively-charged silica substrates. Future experimentation will employ branched polymer systems and seek to justify the use of colloidal stability theories to describe initial capture. Supported by University of Akron, Department of Polymer Science, Department of Biology; LORD Corporation.

  10. Intraocular manifestations of mycobacterium tuberculosis: A review of the literature

    Directory of Open Access Journals (Sweden)

    Lauren A. Dalvin

    2017-05-01

    Full Text Available Mycobacterium tuberculosis: is most commonly associated with pulmonary infection. However, tuberculosis (TB can also affect the eye. TB can affect nearly any tissue in the eye, and a high index of suspicion is required for accurate diagnosis, as many of the intraocular manifestations of TB can mimic other, more common diseases. Correct diagnosis is critical because systemic anti-tuberculosis treatment may be required, and vision loss or even loss of the affected eye can occur without proper treatment. Thus, it is important for ophthalmologists and infectious disease specialists to work together to accurately diagnose and treat intraocular TB. This article reports the various known presentations of intraocular TB and reviews important elements of diagnosis and treatment. Keywords: Mycobacterium, Tuberculosis, Choroidal granuloma, Retinal vasculitis

  11. Siderocalin inhibits the intracellular replication of Mycobacterium tuberculosis in macrophages

    DEFF Research Database (Denmark)

    Johnson, Erin E; Srikanth, Chittur V; Sandgren, Andreas

    2010-01-01

    Siderocalin is a secreted protein that binds to siderophores to prevent bacterial iron acquisition. While it has been shown to inhibit the growth of Mycobacterium tuberculosis (M.tb) in extracellular cultures, its effect on this pathogen within macrophages is not clear. Here, we show that sideroc......Siderocalin is a secreted protein that binds to siderophores to prevent bacterial iron acquisition. While it has been shown to inhibit the growth of Mycobacterium tuberculosis (M.tb) in extracellular cultures, its effect on this pathogen within macrophages is not clear. Here, we show...... findings are consistent with an important role for siderocalin in protection against M.tb infection and suggest that exogenously administered siderocalin may have therapeutic applications in tuberculosis....

  12. Género, cuerpo, racismo y complejo industrial de prisiones: experiencias de personas negras en una cárcel de Bogotá

    Directory of Open Access Journals (Sweden)

    Jei Alanis Bello Ramírez

    2015-12-01

    Full Text Available Este artículo analiza las trayectorias sociales y las experiencias de vida de hombres y mujeres auto-identificados negros y afrocolombianos que se encuentran recluidos en la Cárcel Distrital para varones y anexo de mujeres en la ciudad de Bogotá. A través del trabajo de campo realizado en este penal durante los años 2010 y 2011, y por medio de una reflexión sociológica y feminista, cimentada en los aportes de la feminista afro-estadounidense Angela Davis sobre el “complejo industrial de prisiones”, se pone en evidencia que la intersección de las categorías género, raza y clase articulan las tecnologías de control y punición que emplea el Estado para gestionar la criminalidad en la ciudad. La operación fusionada de estos regímenes de poder configura experiencias diferenciales de criminalización y encarcelamiento para las personas subordinadas en el orden racial colombiano, por lo cual analizo sus trayectorias sociales y sus relaciones con los miembros de la institución carcelaria, para dar cuenta de la discriminación y las resistencias que tejen estos agentes en medio del castigo, el encierro y la criminalización.

  13. Complejo de ejercicios para pacientes con el Síndrome Guillain Barré. Una alternativa en el proceso de rehabilitación

    Directory of Open Access Journals (Sweden)

    Carlos A. Morales Romero

    2011-03-01

    Full Text Available El empleo del ejercicio físico en el tratamiento de enfermedades degenerativas o infecciosas del aparato de sostén y locomotor es un factor fundamental para la formación, desarrollo y reeducación de habilidades motrices y capacidades físicas en los pacientes con estas características. El Síndrome de Guillain Barré es una patología con un largo período de recuperación, en tanto que requiere un elevado volumen de actividad física, por lo que se hace necesario engranar todos los componentes del proceso de rehabilitación, en un programa lógico y coherente que a la vez permita acortar el tiempo de recuperación, y ofrezca la posibilidad de ir constatando la evolución de estos pacientes. Para llevar a cabo la investigación, se aplicó un conjunto de métodos de carácter teórico y empíricos, que dieran la posibilidad de valorar la pertinencia del complejo de ejercicios propuestos.

  14. La polilla Copitarsia decolora: revisión del complejo de especies con base en la morfología genital masculina y de los huevos (Lepidoptera: Noctuidae

    Directory of Open Access Journals (Sweden)

    Andrés O Angulo

    2010-06-01

    Full Text Available Copitarsia decolora es una especie polífaga ampliamente distribuida con un gran número de cambios nominales debido a su plasticidad fenotípica. Examinamos 14 machos de México, Colombia, Venezuela, Perú y Chile y revisamos la literatura producida entre 2003 y 2008. Realizamos un análisis del clasper (digitus de los órganos genitales masculinos del complejo de C. decolora mediante una disección estándar. Utilizamos fotografías de microscopio electrónico de barrido (SEM del corion de los huevos como carácter taxonómico. El género Copitarsia queda compuesto por 23 especies. C. corruda se considera un sinónimo de C. decolora (Guenée.The moth Copitarsia decolora: a review of the species complex based on egg and male genital morphology (Lepidoptera: Noctuidae. Copitarsia decolora is a widely distributed polyphagous species with a large number of nominal changes due to phenotypic plasticity. We reviewed 14 males from Mexico, Colombia, Venezuela, Peru and Chile and the literature produced between 2003 and 2008. An analysis of the clasper complex of C. decolora male genitalia was performed by standard dissection. Scanning electron microscope (SEM photographs of eggs structures were used as taxonomic characters. The genus Copitarsia comprises 23 species. C. corruda is considered a synonym of C. decolora. Rev. Biol. Trop. 58 (2: 769-776. Epub 2010 June 02.

  15. El direccionamiento estratégico y su aplicación en los sistemas complejos y en la gerencia ambiental

    Directory of Open Access Journals (Sweden)

    Iván Alonso Montoya Restrepo

    2003-06-01

    Full Text Available La dirección estratégica ha recorrido un proceso dinámico dentro del cual se han construido diferentes enfoques que van desde modelos de intervención sobre la realidad, hasta enfoques complejos de interacción, relacionados con la construcción y reconocimiento de estrategias que contribuyen a la supervivencia de la organización. Las corrientes conceptuales tienden a ofrecer diversos grados de respuesta ajustada a las necesidades particulares de sectores productivos y a los problemas específicos de toma de decisiones. El presente documento explora la búsqueda de un objeto de estudio definido en el direccionamiento estratégico, los avances en su comprensión, las posibilidades en la composición del área y la definición raíz o básica del campo de estudio, para luego realizar un reconocimiento preliminar de un mapa exploratorio de enfoques y presentar el problema del direccionamiento estratégico ambiental como un desarrollo posterior, con plataforma en la misma dirección estratégica

  16. Propiedades eléctricas en membranas de complejos electrolitos poliméricos PVA-OH/LI2SO4/PEG400

    Directory of Open Access Journals (Sweden)

    Edgar Arbey Villegas

    2014-01-01

    Full Text Available Electrolitos poliméricos conductores de iones de litio, basados en alcohol de polivinilo (PVA-OH complejado con la sal Li2SO4 y diferentes relaciones de porcentaje en peso de plastificante PEG400 fueron preparados por la técnica de solución utilizando agua desionizada como disolvente. El estudio FTIR confirma la formación del complejo polímero-sal. Las curvas de descomposición térmica obtenidas por termogravimetría (TGA muestran que la estabilidad térmica de los electrolitos depende del porcentaje de plastificante. Un proceso de relajación es visible en el formalismo del módulo eléctrico, asociado con la dinámica de la transición vítrea, relajación-α. El máximo de cada pico se desplaza a frecuencias más altas cuando aumenta el plastificante, debido a una mejora de la movilidad dipolar en el origen de los movimientos cooperativos. La dependencia de la parte real de la conductividad eléctrica como función de la frecuencia exhibe una ley de potencias, esta variación es ajustada a la expresión Jonscher.

  17. Nanoparticulas basadas en complejos de Fe(II) con transicion de espin: sintesis, caracterizacion y aplicaciones en electronica molecular

    Science.gov (United States)

    Monrabal Capilla, Maria

    Esta tesis doctoral esta organizada en 5 capitulos y esta destinada al estudio de sistemas de Fe (II) que presentan el fenomeno de la transicion de espin a escala nanometrica. El capitulo 1 contiene una introduccion general sobre materiales moleculares multifuncionales, destacando aquellos ejemplos mas importantes. Por otro lado, se explicara el fenomeno de la transicion de espin, tratando aspectos conceptuales, los antecedentes mas importantes y la situacion actual. En el capitulo 2 se describen los diferentes procesos existentes para la obtencion de diferentes tipos de nanoparticulas. Ademas, se presenta la sintesis y caracterizacion de nanoparticulas del polimero de coordinacion unidimensional [Fe(Htrz)2(trz)]BF4, obtenidas mediante el metodo de micelas inversas. Estas nanoparticulas, con una estrecha distribucion de tamanos centrada alrededor de los 11 nm, presentan una transicion de espin muy abrupta, con un ancho ciclo de histeresis termica de unos 40K. En el capitulo 3 se describe el proceso de modificacion del tamano de las nanoparticulas descritas en el capitulo anterior, llevado a cabo variando la proporcion de surfactante/H2O en el medio. Ademas, con el objetivo de modificar las propiedades magneticas de las nanoparticulas obtenidas en el capitulo 2, se lleva a cabo la sintesis de nanoparticulas de polimeros de la misma familia del [Fe(Htrz)2(trz)]BF4. En concreto se sintetizaron 3 nuevos tipos de nanoparticulas basadas en el polimero [Fe(Htrz)1-x(NH2trz)x](ClO4)2, siendo x = 0.05, 0.15 y 0.3, en cada caso. Estas nanoparticulas siguen presentando una estrecha distribucion de tamanos y una transicion de espin muy abrupta y con un ancho ciclo de histeresis. Ademas, se observa que este ciclo se desplaza a temperaturas mas proximas a la temperatura ambiente a medida que se aumenta el porcentaje de 4-amino-1, 2, 4- triazol en la muestra. Pero al mismo tiempo se produce una disminucion de la anchura de este ciclo. Por ultimo, en este capitulo se presenta la

  18. Biología de Eretmocerus mundus (Hymenoptera: Aphelinidae, parasitoide del complejo Bemisia tabaci (Homoptera: Aleyrodidae, en condiciones de laboratorio

    Directory of Open Access Journals (Sweden)

    Yerlin CHACÓN CASTRO

    2010-01-01

    Full Text Available La estimación de los atributos biológicos de un enemigo natural, previa a su empleo en el campo, constituye un aspecto de importancia en todo proyecto de control biológico. El objetivo de este trabajo fue evaluar los principales parámetros biológicos de Eretmocerus mundus Mercet, parasitoide de Bemisia tabaci (Gennadius, bajo condiciones de laboratorio. La emergencia del adulto, longevidad y fecundidad de la hembra, proporción sexual de la descendencia, tasa intrínseca de crecimiento poblacional (r m , tasa neta de reproducción (R o y tiempo generacional (T; se estimaron mediante la técnica de tabla de vida y fecundidad, utilizando el pimiento ( Capsicum annuum L. y el tomate ( Solanum lycopersicum L. como plantas hospederas. Los resultados obtenidos en pimiento y tomate fueron respectivamente: supervivencia pupal: 86.86 ± 1.94 y 83,45 ± 2,13%; longevidad de la hembra: 18,19 ± 1,61 y 17,00 ± 0,92 días; proporción sexual: 0,34 ± 0,06 y 0,47 ± 0,05 hembras/(machos + hembras; l x50 : 21 y 18 días; r m : 0,226 ± 0,061 y 0,228 ± 0,057 ninfas parasitadas/hembra/día; Ro: 189,71 ± 24,25 y 154,65 ± 17,58 ninfas parasitadas/hembra; T: 25,88 ± 0,42 y 24,03 ± 0,34 días. Los resultados obtenidos son una contribución al conocimiento de la población local del parasitoide y su posible papel como agente de control biológico de B. tabaci.

  19. Isolation of Mycobacterium paratuberculosis from Milk by Immunomagnetic Separation

    OpenAIRE

    Grant, Irene R.; Ball, Hywel J.; Rowe, Michael T.

    1998-01-01

    An immunomagnetic separation (IMS) technique was developed to facilitate selective isolation of Mycobacterium paratuberculosis cells from milk. Rabbit polyclonal antibodies against radiation-killed intact M. paratuberculosis cells were produced and used to coat sheep anti-rabbit immunoglobulin G (IgG) type M-280 Dynabeads. The rabbit anti-M. paratuberculosis IgG-coated beads (IMB) reacted strongly with laboratory strains of M. paratuberculosis as determined by slide agglutination, and microsc...

  20. Cutaneous Mycobacterium abscessus Infection Associated with Mesotherapy Injection.

    Science.gov (United States)

    Wongkitisophon, Pranee; Rattanakaemakorn, Ploysyne; Tanrattanakorn, Somsak; Vachiramon, Vasanop

    2011-02-18

    Non-tuberculous mycobacterial skin infections have an increasing incidence. In immunocompetent patients, they usually follow local trauma. We present a case of cutaneous Mycobacterium abscessus infection following mesotherapy. The lesions were successfully treated with a combination of clarithromycin, ciprofloxacin, and doxycycline. Atypical mycobacterial infection should be suspected in patients who develop late-onset skin and soft tissue infection after cutaneous injury, injection, and surgical intervention, particularly if they do not respond to conventional antibiotic treatment.

  1. Cutaneous Mycobacterium abscessus Infection Associated with Mesotherapy Injection

    Directory of Open Access Journals (Sweden)

    Pranee Wongkitisophon

    2011-02-01

    Full Text Available Non-tuberculous mycobacterial skin infections have an increasing incidence. In immunocompetent patients, they usually follow local trauma. We present a case of cutaneous Mycobacterium abscessus infection following mesotherapy. The lesions were successfully treated with a combination of clarithromycin, ciprofloxacin, and doxycycline. Atypical mycobacterial infection should be suspected in patients who develop late-onset skin and soft tissue infection after cutaneous injury, injection, and surgical intervention, particularly if they do not respond to conventional antibiotic treatment.

  2. Bloodstream Infections with Mycobacterium tuberculosis among HIV patients

    Centers for Disease Control (CDC) Podcasts

    2010-09-23

    This podcast looks at bloodstream infections with Mycobacterium tuberculosis and other pathogens among outpatients infected with HIV in Southeast Asia. CDC health scientist Kimberly McCarthy discusses the study and why bloodstream infections occur in HIV-infected populations.  Created: 9/23/2010 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 9/23/2010.

  3. Mycobacterium avium-intracellulare: a rare cause of subacromial bursitis.

    Science.gov (United States)

    Sinha, Raj; Tuckett, John; Hide, Geoff; Dildey, Petra; Karsandas, Alvin

    2015-01-01

    Septic subacromial bursitis is an uncommon disorder with only a few reported cases in the literature. The most common causative organism is Staphylococcus aureus. We report the case of a 61-year-old female with a septic subacromial bursitis where the causative organism was found to be Mycobacterium avium-intracellulare (MAI). The diagnosis was only made following a biopsy, and we use this case to highlight the importance of recognising the need to consider a biopsy and aspiration in atypical situations.

  4. Structural and Functional Studies of Phosphoenolpyruvate Carboxykinase from Mycobacterium tuberculosis

    Czech Academy of Sciences Publication Activity Database

    Machová, Iva; Snášel, Jan; Dostál, Jiří; Brynda, Jiří; Fanfrlík, Jindřich; Singh, M.; Tarábek, Ján; Vaněk, O.; Bednárová, Lucie; Pichová, Iva

    2015-01-01

    Roč. 10, č. 3 (2015), e0120682/1-e0120682/21 E-ISSN 1932-6203 R&D Projects: GA MŠk LO1302 EU Projects: European Commission(XE) 241587 - SYSTEMTB Institutional support: RVO:61388963 Keywords : crystal structure * noncovalent complexes * Mycobacterium tuberculosis * mechanism Subject RIV: CE - Biochemistry Impact factor: 3.057, year: 2015 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0120682

  5. Studium role fosfofruktokinasy A a B v metabolismu Mycobacterium tuberculosis

    Czech Academy of Sciences Publication Activity Database

    Machová, Iva; Snášel, Jan; Pichová, Iva

    2014-01-01

    Roč. 108, č. 5 (2014), s. 542 ISSN 0009-2770. [Mezioborové setkání mladých biologů, biochemiků a chemiků /14./. 13.05.2014-16.05.2014, Milovy] Grant - others:European Research Council(XE) FP7-245187 Institutional support: RVO:61388963 Keywords : Mycobacterium tuberculosis * phosphofructokinase Subject RIV: CE - Biochemistry

  6. Tumor necrosis factor blockers influence macrophage responses to Mycobacterium tuberculosis

    OpenAIRE

    HARRIS, JAMES; HARRIS, JAMES

    2008-01-01

    PUBLISHED umor necrosis factor (TNF)?? is a proinflammatory cytokine that mediates inflammation in response to various pathogens, including Mycobacterium tuberculosis, but is also a key factor in the pathogenesis of rheumatoid arthritis and other autoimmune diseases. Three TNF???suppressing drugs have been approved to treat selected autoimmune diseases; 2 are monoclonal antibodies against TNF?? (adalimumab and infliximab), and the other is a soluble TNF receptor/Fc fusion protein (etanerce...

  7. Disseminated Mycobacterium abscessus infection in a peritoneal dialysis patient

    Directory of Open Access Journals (Sweden)

    Vincent H.J.F. Mooren

    2017-01-01

    Full Text Available A disseminated peritoneal dialysis-related Mycobacterium abscessus infection is very rare. M. abscessus belongs to the rapidly growing mycobacteria and can be misidentified as a diphtheroid bacterium, which in our case delayed diagnosis and optimal treatment. Due to intrinsic resistance to most antimicrobials, therapeutic options in M. abscessus infections are limited. Infection often leads to catheter loss. A fatal outcome, like in our case, is not exceptional.

  8. Mycobacterium bovis in milk samples: a preliminary investigation using PCR

    International Nuclear Information System (INIS)

    Achel, D.G.; Gyamfi, O.K.; Broni, F.; Gomda, Y.; Brown, C.A.

    2007-01-01

    PCR was used to screen milk samples (n=41) for Mycobacterium bovis. DNA samples were obtained through concentration by 50% sucrose addition and centrifugation. Sixteen (16) samples (or 39%) were positive for M. Bovis DNA and the rest 25 (or 61%) were negative. All four kraals had some samples testing positive for M. bovis; the highest being 50% (5/10) and the lowest being 13% (2/15). (au)

  9. Evolutionary landscape of the Mycobacterium tuberculosis complex from the viewpoint of PhoPR: implications for virulence regulation and application to vaccine development.

    Science.gov (United States)

    Broset, Esther; Martín, Carlos; Gonzalo-Asensio, Jesús

    2015-10-20

    Different members of the Mycobacterium genus have evolved to cause tuberculosis in diverse human populations and in a variety of animal species. Our cumulative knowledge of mycobacterial genomes indicates that mutations in the PhoPR two-component virulence system were acquired not only during the natural evolution of mycobacterial species but also during in vitro subculture, which has given rise to the attenuated reference strain H37Ra or to different daughter strains of Mycobacterium bovis BCG. PhoPR is a well-known regulator of pathogenic phenotypes, including secretion of the virulence factor ESAT-6, biosynthesis of acyltrehalose-based lipids, and modulation of antigen export, in members of the Mycobacterium tuberculosis complex (MTBC). Evolutionarily conserved polymorphisms in PhoPR from Mycobacterium africanum, M. bovis, or M. tuberculosis H37Ra result in loss of functional phenotypes. Interestingly, some members of the MTBC have acquired compensatory mutations to counteract these polymorphisms and, probably, to maintain their pathogenic potential. Some of these compensatory mutations include the insertion of the IS6110 element upstream from phoPR in a particular M. bovis strain that is able to transmit between humans or polymorphisms in M. africanum and M. bovis that affect the regulatory region of the espACD operon, allowing PhoPR-independent ESAT-6 secretion. This review highlights the increasing knowledge of the significance of PhoPR in the evolution of the MTBC and its potential application in the construction of new attenuated vaccines based on phoPR inactivation. In this context, the live attenuated vaccine MTBVAC, based on a phoP fadD26 deletion mutant of M. tuberculosis, is the first vaccine of this kind to successfully enter into clinical development, representing a historic milestone in the field of human vaccinology. Copyright © 2015 Broset et al.

  10. Protective Effect of a Lipid-Based Preparation from Mycobacterium smegmatis in a Murine Model of Progressive Pulmonary Tuberculosis

    Directory of Open Access Journals (Sweden)

    Maria de los Angeles García

    2014-01-01

    Full Text Available A more effective vaccine against tuberculosis (TB is urgently needed. Based on its high genetic homology with Mycobacterium tuberculosis (Mtb, the nonpathogenic mycobacteria, Mycobacterium smegmatis (Ms, could be an attractive source of potential antigens to be included in such a vaccine. We evaluated the capability of lipid-based preparations obtained from Ms to provide a protective response in Balb/c mice after challenge with Mtb H37Rv strain. The intratracheal model of progressive pulmonary TB was used to assess the level of protection in terms of bacterial load as well as the pathological changes in the lungs of immunized Balb/c mice following challenge with Mtb. Mice immunized with the lipid-based preparation from Ms either adjuvanted with Alum (LMs-AL or nonadjuvanted (LMs showed significant reductions in bacterial load (P<0.01 compared to the negative control group (animals immunized with phosphate buffered saline (PBS. Both lipid formulations showed the same level of protection as Bacille Calmette and Guerin (BCG. Regarding the pathologic changes in the lungs, mice immunized with both lipid formulations showed less pneumonic area when compared with the PBS group (P<0.01 and showed similar results compared with the BCG group. These findings suggest the potential of LMs as a promising vaccine candidate against TB.

  11. First description of Mycobacterium tuberculosis Beijing genotype in Argentina Primera descripción del genotipo Beijing de Mycobacterium tuberculosis en la Argentina

    Directory of Open Access Journals (Sweden)

    N. Morcillo

    2005-06-01

    Full Text Available During a population-based study to genotype isolates of Mycobacterium tuberculosis from Buenos Aires Northern suburbs, we found isolates with molecular patterns related to those of the Beijing genotype. Five out of 590 (0.85% patients had isolates with spoligopattern identical to that of the Beijing family. Since two of these isolates showed identical IS6110RFLP pattern, we found only four different patterns containing 11 to 19 bands. The isolates were obtained from young people (including a 7 years-old child who were born in Argentina, and were living in a small area of our region. However, conventional contact tracing did not prove epidemiological linkage among them. These isolates were fully drug-susceptible to the first-line drugs. The comparison of the IS6110RFLP patterns from our isolates against a set of 19 reference Beijing patterns from the RIVM (The Netherlands confirmed that the strains belonged to the Beijing lineage. These findings might be partially explained by the important migration phenomena occurred during the last decade. Further surveillance studies would help in the following of Beijing family strain dissemination in our community.Durante un estudio poblacional para genotipificar aislamientos de Mycobacterium tuberculosis de Gran Buenos Aires Norte, se encontraron aislamientos con patrones moleculares relacionados con el genotipo Beijing. Cinco de 590 (0,85% pacientes tuvieron aislamientos con un patrón de spoligotyping idéntico al de la familia Beijing. Dos de esos aislamientos mostraron un patrón de IS6110 RFLP idéntico, por lo que finalmente fueron encontrados cuatro patrones diferentes conteniendo entre 11 y 19 bandas. Las cepas fueron aisladas de personas jóvenes incluyendo un niño de siete años de edad, todos ellos nacidos en Argentina y residentes en un pequeño distrito de nuestra región. Sin embargo, no pudo hallarse ninguna conexión epidemiológica entre ellos. La comparación de los patrones de IS6110

  12. Triple valve endocarditis by mycobacterium tuberculosis. A case report

    Directory of Open Access Journals (Sweden)

    Shaikh Quratulain

    2012-09-01

    Full Text Available Abstract Background Granulomas caused by Mycobacterium Tuberculosis have been observed at autopsy in the heart, pre-dominantly in the myocardium and endocardium, but rarely involving the coronary vessels and valvular structures. Mycobacterium tuberculosis valvular endocarditis is extremely rare, with most reports coming from autopsy series. Case presentation We report the case of a 17 year old immunocompetent girl who presented with history of fever, malaise, foot gangrene and a left sided hemiparesis. On investigation she was found to have infective endocarditis involving the aortic, mitral and tricuspid valves. She had developed a right middle cerebral artery stroke. She underwent dual valve replacement and tricuspid repair. The vegetations showed granulomatous inflammation but blood cultures and other biological specimen cultures were negative for any organisms. She was started on antituberculous treatment and anticoagulation. Conclusion This is the first reported case of triple valve endocarditis by Mycobacterium Tuberculosis in an immunocompetent host. Especially important is the fact that the right heart is involved which has been historically described in the setting of intravenous drug abuse. This implies that Tuberculosis should be considered in cases of culture negative endocarditis in endemic areas like Pakistan even in immunocompetent hosts.

  13. Infection due to Mycobacterium bovis in common variable immunodeficiency

    Directory of Open Access Journals (Sweden)

    Diana Andrea Herrera-Sánchez

    2015-02-01

    Full Text Available Common variable immunodeficiency (CVID is an heterogeneous group of disorders characterized by impaired antibody production. It shows a wide spectrum of manifestations including severe and recurrent respiratory infections (Streptococcus pneumoniae, Haemophilus and gastrointestinal (Campylobacter jejuni, rotavirus and Giardia lamblia. Viral infections caused by herpes zoster, cytomegalovirus (CMV and hepatitis C are rare. The opportunistic agents such as CMV, Pneumocystis jirovecii, cryptococcus and atypical mycobacteria have been reported as isolated cases. This paper reports the case of a 38-year-old female patient, who began six years before with weight loss of 7 kg in six months, fatigue, weakness, sweating, fever and abdominal pain. Furthermore, patient had intestinal obstruction and abdominal CT showed mesenteric lymph growth. The mesenteric lymph node biopsy revealed positives Mycobacterium PCR, Ziehl-Neelsen staining and culture for M. bovis. In the laparotomy postoperative period was complicated with nosocomial pneumonia, requiring mechanical ventilation and tracheostomy. Two years later, she developed right renal abscess that required surgical drainage, once again with a positive culture for Mycobacterium bovis. She was referred to highly specialized hospital and we documented panhypogammaglobulinemia and lymphopenia. Secondary causes of hypogammaglobulinemia were ruled out and common variable immunodeficiency (CVID was confirmed, we started IVIG replacement. Four years later she developed mixed cellularity Hodgkin’s lymphoma. Until today she continues with IVIG and chemotherapy. This report of a patient with CVID and Mycobacterium bovis infection, a unusual association, shows the cellular immunity susceptibility in this immunodeficiency, additional to the humoral defect.

  14. Expresión de las moléculas del Complejo Mayor de Histocompatibilidad clase II y moléculas co-estimuladoras en carcinomas orales in vitro

    OpenAIRE

    Villarroel, Mariana; Speight, Paul M.; Barrett, A. William

    2005-01-01

    El descubrimiento de que el epitelio escamoso estratificado que cubre la mucosa oral podia expresar moleculas del Complejo Mayor de Histocompatibilidad clase II en varias condiciones patologicas de tipo inflamatorio abrio la posibilidad de que los queratinocitos orales sean celulas inmunologicamente activas, las cuales pueden funcionar con .celulas presentadoras de antigenos'ñ. Para una efectiva activacion de los linfocitos T, las celulas presentadoras de antigenos requie...

  15. EL SUELO AL SERVICIO DE LA SOCIEDAD Y SU ROL EN EL CONTEXTO DE LOS CAMBIOS GLOBALES

    Directory of Open Access Journals (Sweden)

    Hernán Burbano Orjuela

    2010-07-01

    Full Text Available Este artículo examina la relación entre suelo, vida y sociedad y destaca que el primero es el soporte del complejo sistema propio del planeta. Señala que en las disfunciones del sistema terrestre se inscriben los problemas del suelo. Alude a los cambios globales contemporáneos y al rol del suelo en este contexto. Y, finalmente, destaca que el suelo se ubica en un planeta que funciona como un sistema que se debe mantener en condiciones adecuadas para la vida.

  16. Mycobacterium stephanolepidis sp. nov., a rapidly growing species related to Mycobacterium chelonae, isolated from marine teleost fish, Stephanolepis cirrhifer.

    Science.gov (United States)

    Fukano, Hanako; Wada, Shinpei; Kurata, Osamu; Katayama, Kinya; Fujiwara, Nagatoshi; Hoshino, Yoshihiko

    2017-08-01

    A previously undescribed rapidly growing, non-pigmented mycobacterium was identified based on biochemical and nucleic acid analyses, as well as growth characteristics. Seven isolates were cultured from samples collected from five thread-sail filefish (Stephanolepis cirrhifer) and two farmed black scraper (Thamnaconus modestus). Bacterial growth occurred at 15-35 °C on Middlebrook 7H11 agar. The bacteria were positive for catalase activity at 68 °C and urease activity, intermediate for iron uptake, and negative for Tween 80 hydrolysis, nitrate reduction, semi-quantitative catalase activity and arylsulfatase activity at day 3. No growth was observed on Middlebrook 7H11 agar supplemented with picric acid, and very little growth was observed in the presence of 5 % NaCl. α- and α'-mycolates were identified in the cell walls, and a unique profile of the fatty acid methyl esters and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiles of the protein and cell-wall lipids were acquired. Sequence analysis revealed that the seven isolates shared identical sequences for the 16S rRNA, rpoB, hsp65, recA and sodA genes. Phylogenetic analysis of the five gene sequences confirmed that the isolates were unique, but closely related to Mycobacterium chelonae. Antibiotic susceptibility testing revealed the minimum inhibitory concentration (MIC) of clarithromycin against this novel species was Mycobacterium salmoniphilum. The hsp65 PCR restriction enzyme analysis pattern differed from those of M. chelonae and M. salmoniphilum. Based on these findings, the name Mycobacterium stephanolepidis sp. nov. is proposed for this novel species, with the type strain being NJB0901 T (=JCM 31611 T =KCTC 39843 T ).

  17. Las cuencas cenozoicas y su control en el volcanismo de los Complejos Nevados de Chillan y Copahue-Callaqui (Andes del Sur, 36-39°S Cenozoic basins and their control on volcanism of Nevados de Chillan and Copahue-Callaqui complexes (36-39°S, Southern Andes

    Directory of Open Access Journals (Sweden)

    Juan Pablo Radic

    2010-01-01

    Full Text Available La Cordillera Principal de los Andes entre los 36° y 39°S constituye un excelente lugar para el estudio de la relación entre volcanismo y tectónica. Por medio del análisis tectonoestratigráfico de las cuencas cenozoicas se ha podido reconocer una clara coincidencia espacial entre estructuras de primer orden pertenecientes a estas cuencas y la distribución del volcanismo plio-pleistocénico. Las cuencas cenozoicas comenzaron su desarrollo durante el Oligoceno-Mioceno como depresiones extensionales, conformando un sistema de al menos tres subcuencas orientadas N-S y conectadas por dos zonas de acomodación estructural. Posteriormente este sistema de cuencas fue tectónicamente invertido hacia finales del Mioceno y los antiguos depocentros fueron deformados y exhumados, reutilizando las fallas originalmente extensionales. Las zonas de acomodación estructural permanecieron como estructuras de primer orden a escala de cuenca y constituyeron zonas de debilidad que favorecieron el posterior desarrollo del volcanismo y magma-tismo en los complejos volcánicos Nevados de Chillan y Copahue-Callaqui. Cada uno de estos complejos volcánicos se caracteriza por una orientación de sus centros de emisión, morfología y depósitos volcánicos en forma subparalela a la orientación de las zonas de acomodación estructural inmediatamente por debajo.The Cordillera Principal at 36° to 39°S is an excellent place to address the relationship between tectonics and volcanism. Based on tectonostratigraphic analysis of the Cenozoic basins it has been possible to recognize a clear spatial coincidence between first order basin scale structures and Plio-Pleistocene volcanism. Cenozoic basins started in the Oligocene-Miocene as extensional depocenters, characterized by at least three north-south oriented sub-basins connected by two structural accommodation zones. Towards the Late Miocene this extensional system was tectonically inverted deforming and uplifting the

  18. Protective and therapeutic efficacy of Mycobacterium smegmatis expressing HBHA-hIL12 fusion protein against Mycobacterium tuberculosis in mice.

    Directory of Open Access Journals (Sweden)

    Shanmin Zhao

    Full Text Available Tuberculosis (TB remains a major worldwide health problem. The only vaccine against TB, Mycobacterium bovis Bacille Calmette-Guerin (BCG, has demonstrated relatively low efficacy and does not provide satisfactory protection against the disease. More efficient vaccines and improved therapies are urgently needed to decrease the worldwide spread and burden of TB, and use of a viable, metabolizing mycobacteria vaccine may be a promising strategy against the disease. Here, we constructed a recombinant Mycobacterium smegmatis (rMS strain expressing a fusion protein of heparin-binding hemagglutinin (HBHA and human interleukin 12 (hIL-12. Immune responses induced by the rMS in mice and protection against Mycobacterium tuberculosis (MTB were investigated. Administration of this novel rMS enhanced Th1-type cellular responses (IFN-γ and IL-2 in mice and reduced bacterial burden in lungs as well as that achieved by BCG vaccination. Meanwhile, the bacteria load in M. tuberculosis infected mice treated with the rMS vaccine also was significantly reduced. In conclusion, the rMS strain expressing the HBHA and human IL-12 fusion protein enhanced immunogencity by improving the Th1-type response against TB, and the protective effect was equivalent to that of the conventional BCG vaccine in mice. Furthermore, it could decrease bacterial load and alleviate histopathological damage in lungs of M. tuberculosis infected mice.

  19. Mycobacterium alsiense, a novel, slowly growing species isolated from two patients with pulmonary disease

    DEFF Research Database (Denmark)

    Richter, Elvira; Tortoli, Enrico; Fischer, Arno

    2007-01-01

    A previously undescribed, slowly growing Mycobacterium species was isolated from pulmonary specimens of two patients, one from Denmark and one from Italy. The isolates showed unique 16S rRNA internal transcribed spacers and hsp65 sequences: the 16S rRNA was most closely related to Mycobacterium...

  20. Comparative genomics of archived pyrazinamide resistant Mycobacterium tuberculosis complex isolates from Uganda

    Science.gov (United States)

    Bovine tuberculosis is a ‘neglected zoonosis’ and its contribution to the proportion of Mycobacterium tuberculosis complex infections in humans is unknown. A retrospective study on archived Mycobacterium tuberculosis complex (MTC) isolates from a reference laboratory in Uganda was undertaken to iden...

  1. Pott's disease: a case of Mycobacterium xenopi infection of the spine

    Directory of Open Access Journals (Sweden)

    Majd Alfreijat

    2013-01-01

    Full Text Available Pott's disease is an infection of the spine with Mycobacterium tuberculosis that causes destruction of the spine elements resulting in progressive kyphosis. We are describing a rare case of Pott's disease where Mycobacterium xenopi was the inculpated organism.

  2. Diffuse Lepromatous Leprosy Due to Mycobacterium lepromatosis in Quintana Roo, Mexico.

    Science.gov (United States)

    Han, Xiang Y; Quintanilla, Marco

    2015-11-01

    A 43-year-old woman of Mayan origin from Quintana Roo, Mexico, was diagnosed with diffuse lepromatous leprosy. The etiologic bacillus was determined to be Mycobacterium lepromatosis instead of Mycobacterium leprae. This case likely represents the first report of this leprosy form and its agent in the southeastern tip of Mexico. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  3. Environmental Mycobacterium avium subsp. paratuberculosis hosted by free-living amoebae

    Science.gov (United States)

    Mycobacterium avium subsp. paratuberculosis is responsible for paratuberculosis in animals. This disease, leading to an inflammation of the gastrointestinal tract, has a high impact on animal health and an important economic burden. The environmental life cycle of Mycobacterium avium subsp. paratube...

  4. Performance Assessment of the CapitalBio Mycobacterium Identification Array System for Identification of Mycobacteria

    Science.gov (United States)

    Liu, Jingbo; Yan, Zihe; Han, Min; Han, Zhijun; Jin, Lingjie; Zhao, Yanlin

    2012-01-01

    The CapitalBio Mycobacterium identification microarray system is a rapid system for the detection of Mycobacterium tuberculosis. The performance of this system was assessed with 24 reference strains, 486 Mycobacterium tuberculosis clinical isolates, and 40 clinical samples and then compared to the “gold standard” of DNA sequencing. The CapitalBio Mycobacterium identification microarray system showed highly concordant identification results of 100% and 98.4% for Mycobacterium tuberculosis complex (MTC) and nontuberculous mycobacteria (NTM), respectively. The sensitivity and specificity of the CapitalBio Mycobacterium identification array for identification of Mycobacterium tuberculosis isolates were 99.6% and 100%, respectively, for direct detection and identification of clinical samples, and the overall sensitivity was 52.5%. It was 100% for sputum, 16.7% for pleural fluid, and 10% for bronchoalveolar lavage fluid, respectively. The total assay was completed in 6 h, including DNA extraction, PCR, and hybridization. The results of this study confirm the utility of this system for the rapid identification of mycobacteria and suggest that the CapitalBio Mycobacterium identification array is a molecular diagnostic technique with high sensitivity and specificity that has the capacity to quickly identify most mycobacteria. PMID:22090408

  5. Characterization of Three Mycobacterium spp. with Potential Use in Bioremediation by Genome Sequencing and Comparative Genomics.

    Science.gov (United States)

    Das, Sarbashis; Pettersson, B M Fredrik; Behra, Phani Rama Krishna; Ramesh, Malavika; Dasgupta, Santanu; Bhattacharya, Alok; Kirsebom, Leif A

    2015-06-16

    We provide the genome sequences of the type strains of the polychlorophenol-degrading Mycobacterium chlorophenolicum (DSM43826), the degrader of chlorinated aliphatics Mycobacterium chubuense (DSM44219) and Mycobacterium obuense (DSM44075) that has been tested for use in cancer immunotherapy. The genome sizes of M. chlorophenolicum, M. chubuense, and M. obuense are 6.93, 5.95, and 5.58 Mb with GC-contents of 68.4%, 69.2%, and 67.9%, respectively. Comparative genomic analysis revealed that 3,254 genes are common and we predicted approximately 250 genes acquired through horizontal gene transfer from different sources including proteobacteria. The data also showed that the biodegrading Mycobacterium spp. NBB4, also referred to as M. chubuense NBB4, is distantly related to the M. chubuense type strain and should be considered as a separate species, we suggest it to be named Mycobacterium ethylenense NBB4. Among different categories we identified genes with potential roles in: biodegradation of aromatic compounds and copper homeostasis. These are the first nonpathogenic Mycobacterium spp. found harboring genes involved in copper homeostasis. These findings would therefore provide insight into the role of this group of Mycobacterium spp. in bioremediation as well as the evolution of copper homeostasis within the Mycobacterium genus. © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  6. Progression to active tuberculosis, but not transmission, varies by Mycobacterium tuberculosis lineage in The Gambia

    NARCIS (Netherlands)

    de Jong, Bouke C.; Hill, Philip C.; Aiken, Alex; Awine, Timothy; Antonio, Martin; Adetifa, Ifedayo M.; Jackson-Sillah, Dolly J.; Fox, Annette; Deriemer, Kathryn; Gagneux, Sebastien; Borgdorff, Martien W.; McAdam, Keith P. W. J.; Corrah, Tumani; Small, Peter M.; Adegbola, Richard A.

    2008-01-01

    BACKGROUND: There is considerable variability in the outcome of Mycobacterium tuberculosis infection. We hypothesized that Mycobacterium africanum was less likely than M. tuberculosis to transmit and progress to tuberculosis disease. METHODS: In a cohort study of patients with tuberculosis and their

  7. Comparative analysis of mycobacterium and related actinomycetes yields insight into the evolution of mycobacterium tuberculosis pathogenesis

    Directory of Open Access Journals (Sweden)

    McGuire Abigail

    2012-03-01

    Full Text Available Abstract Background The sequence of the pathogen Mycobacterium tuberculosis (Mtb strain H37Rv has been available for over a decade, but the biology of the pathogen remains poorly understood. Genome sequences from other Mtb strains and closely related bacteria present an opportunity to apply the power of comparative genomics to understand the evolution of Mtb pathogenesis. We conducted a comparative analysis using 31 genomes from the Tuberculosis Database (TBDB.org, including 8 strains of Mtb and M. bovis, 11 additional Mycobacteria, 4 Corynebacteria, 2 Streptomyces, Rhodococcus jostii RHA1, Nocardia farcinia, Acidothermus cellulolyticus, Rhodobacter sphaeroides, Propionibacterium acnes, and Bifidobacterium longum. Results Our results highlight the functional importance of lipid metabolism and its regulation, and reveal variation between the evolutionary profiles of genes implicated in saturated and unsaturated fatty acid metabolism. It also suggests that DNA repair and molybdopterin cofactors are important in pathogenic Mycobacteria. By analyzing sequence conservation and gene expression data, we identify nearly 400 conserved noncoding regions. These include 37 predicted promoter regulatory motifs, of which 14 correspond to previously validated motifs, as well as 50 potential noncoding RNAs, of which we experimentally confirm the expression of four. Conclusions Our analysis of protein evolution highlights gene families that are associated with the adaptation of environmental Mycobacteria to obligate pathogenesis. These families include fatty acid metabolism, DNA repair, and molybdopterin biosynthesis. Our analysis reinforces recent findings suggesting that small noncoding RNAs are more common in Mycobacteria than previously expected. Our data provide a foundation for understanding the genome and biology of Mtb in a comparative context, and are available online and through TBDB.org.

  8. Comparative analysis of Mycobacterium and related Actinomycetes yields insight into the evolution of Mycobacterium tuberculosis pathogenesis.

    Science.gov (United States)

    McGuire, Abigail Manson; Weiner, Brian; Park, Sang Tae; Wapinski, Ilan; Raman, Sahadevan; Dolganov, Gregory; Peterson, Matthew; Riley, Robert; Zucker, Jeremy; Abeel, Thomas; White, Jared; Sisk, Peter; Stolte, Christian; Koehrsen, Mike; Yamamoto, Robert T; Iacobelli-Martinez, Milena; Kidd, Matthew J; Maer, Andreia M; Schoolnik, Gary K; Regev, Aviv; Galagan, James

    2012-03-28

    The sequence of the pathogen Mycobacterium tuberculosis (Mtb) strain H37Rv has been available for over a decade, but the biology of the pathogen remains poorly understood. Genome sequences from other Mtb strains and closely related bacteria present an opportunity to apply the power of comparative genomics to understand the evolution of Mtb pathogenesis. We conducted a comparative analysis using 31 genomes from the Tuberculosis Database (TBDB.org), including 8 strains of Mtb and M. bovis, 11 additional Mycobacteria, 4 Corynebacteria, 2 Streptomyces, Rhodococcus jostii RHA1, Nocardia farcinia, Acidothermus cellulolyticus, Rhodobacter sphaeroides, Propionibacterium acnes, and Bifidobacterium longum. Our results highlight the functional importance of lipid metabolism and its regulation, and reveal variation between the evolutionary profiles of genes implicated in saturated and unsaturated fatty acid metabolism. It also suggests that DNA repair and molybdopterin cofactors are important in pathogenic Mycobacteria. By analyzing sequence conservation and gene expression data, we identify nearly 400 conserved noncoding regions. These include 37 predicted promoter regulatory motifs, of which 14 correspond to previously validated motifs, as well as 50 potential noncoding RNAs, of which we experimentally confirm the expression of four. Our analysis of protein evolution highlights gene families that are associated with the adaptation of environmental Mycobacteria to obligate pathogenesis. These families include fatty acid metabolism, DNA repair, and molybdopterin biosynthesis. Our analysis reinforces recent findings suggesting that small noncoding RNAs are more common in Mycobacteria than previously expected. Our data provide a foundation for understanding the genome and biology of Mtb in a comparative context, and are available online and through TBDB.org.

  9. Mycobacterium avium-intracellulare cellulitis occurring with septic arthritis after joint injection: a case report

    Directory of Open Access Journals (Sweden)

    Murdoch David M

    2007-02-01

    Full Text Available Abstract Background Cellulitis caused by Mycobacterium avium-intracellulare has rarely been described. Mycobacterium avium-intracellulare is a rare cause of septic arthritis after intra-articular injection, though the causative role of injection is difficult to ascertain in such cases. Case presentation A 57-year-old with rheumatoid arthritis treated with prednisone and azathioprine developed bilateral painful degenerative shoulder arthritis. After corticosteroid injections into both acromioclavicular joints, he developed bilateral cellulitis centered over the injection sites. Skin biopsy showed non-caseating granulomas, and culture grew Mycobacterium avium-intracellulare. Joint aspiration also revealed Mycobacterium avium-intracellulare infection. Conclusion Although rare, skin and joint infections caused by Mycobacterium avium-intracellulare should be considered in any immunocompromised host, particularly after intra-articular injection. Stains for acid-fast bacilli may be negative in pathologic samples even in the presence of infection; cultures of tissue specimens should always be obtained.

  10. Mycobacterium eburneum sp. nov., a non-chromogenic, fast-growing strain isolated from sputum.

    Science.gov (United States)

    Nouioui, Imen; Carro, Lorena; Teramoto, Kanae; Igual, José M; Jando, Marlen; Del Carmen Montero-Calasanz, Maria; Sutcliffe, Iain; Sangal, Vartul; Goodfellow, Michael; Klenk, Hans-Peter

    2017-09-01

    A polyphasic study was undertaken to establish the taxonomic position of a non-chromogenic, rapidly growing Mycobacterium strain that had been isolated from sputum. The strain, CECT 8775T, has chemotaxonomic and cultural properties consistent with its classification in the genus Mycobacterium and was distinguished from the type strains of closely related mycobacterial species, notably from Mycobacterium paraense DSM 46749T, its nearest phylogenetic neighbour, based on 16S rRNA, hsp65 and rpoB gene sequence data. These organisms were also distinguished by a broad range of chemotaxonomic and phenotypic features and by a digital DNA-DNA relatedness value of 22.8 %. Consequently, the strain is considered to represent a novel species of Mycobacterium for which the name Mycobacterium eburneum sp. nov is proposed; the type strain is X82T (CECT 8775T=DSM 44358T).

  11. Mycobacterium shigaense Causes Lymph Node and Cutaneous Lesions as Immune Reconstitution Syndrome in an AIDS Patient: The Third Case Report of a Novel Strain Non-tuberculous Mycobacterium

    Science.gov (United States)

    Koizumi, Yusuke; Shimizu, Kaoru; Shigeta, Masayo; Minamiguchi, Hitoshi; Hodohara, Keiko; Andoh, Akira; Tanaka, Toshihide; Chikamatsu, Kinuyo; Mitarai, Satoshi; Mikamo, Hiroshige

    2016-01-01

    A 40-year-old man complaining of progressive body weight loss was diagnosed to have acquired immunodeficiency syndrome. Within 2 weeks after the initiation of combination antiretroviral therapy, he developed fever, massive cervical lymphadenopathy and a protruding subcutaneous abscess. A lymph node biopsy and abscess drainage revealed non-caseous granuloma and mycobacterium. The mycobacterium belonged to Runyon II group, but it showed no matches to any previously reported species. According to sequence analyses, the strain was identified as Mycobacterium shigaense. After six months of antimycobacterial treatment, the lesions were all successfully cured. This is the third case report of the novel mycobacterium, M. shigaense, presenting in associatioin with immune reconstitution syndrome. PMID:27853087

  12. Subvolcanic contact metasomatism at El Laco Volcanic Complex, Central Andes Metasomatismo de contacto subvolcánico en el Complejo Volcánico El Laco, Andes centrales

    Directory of Open Access Journals (Sweden)

    José A Naranjo

    2010-01-01

    Full Text Available Studies of drill cores from the Pasos Blancos area at El Laco in the central Andes, northern Chile, give evidence of an intense and extensive subvolcanic contact-metasomatic process. This process resulted from shallow-level emplacement of very volatile-rich iron-oxide magma, with discharge of volatiles that resulted in extensive fracturing of overlying volcanic rocks. The brecciated rocks were altered (mainly extensive scapolitization and formation of pyroxene by hot magmatic fluids emitted from the cooling intrusion , and accompanied by magnetite deposition. With time and decreasing temperature, the metasomatic fluids evolved to fluids of hydrothermal character, and a final recent geothermal event took place that deposited superficial gypsum over a large part of the El Laco Volcanic Complex.Estudios realizados en testigos de sondajes en el area de Pasos Blancos en El Laco, en los Andes Centrales del norte de Chile, dan evidencias de un intenso y extenso proceso subvolcánico de metasomatismo de contacto. Este proceso es el resultado de un emplazamiento a poca profundidad de un magma de óxido de fierro muy rico en volátiles y cuya descarga de gases produjo un intenso fracturamiento de las rocas sobrepuestas. Las rocas brechizadas fueron alteradas (principalmente una extensa escapolitización y formación de piroxeno, junto con la depositación de magnetita, por los fluidos magmáticos calientes emitidos por la intrusión durante su enfriamiento. Los fluidos metasomáticos evolucionaron en el tiempo y con la disminución de temperatura, a fluidos de carácter hidrotermal y finalmente tuvo lugar un evento geotérmico reciente, el cual depositó yeso superficial en gran parte del Complejo Volcánico El Laco.

  13. Manejo del enoftalmos como secuela de fracturas del complejo cigomático-orbitario con apoyo de estereolitografía

    Directory of Open Access Journals (Sweden)

    H.

    Full Text Available El enoftalmos postraumático se define como la discrepancia entre el volumen orbitario y su contenido; este balance puede verse alterado por múltiples factores, como el secuestro tardío del contenido orbitario por atrapamiento en el sitio de la fractura, herniación del contenido orbitario, necrosis del tejido adiposo orbitario, contracción cicatricial del tejido retrobulbar, pérdida del sistema de suspensión ligamentario del globo ocular y aumento de volumen de la cavidad orbitaria. El presente estudio aborda el problema del enoftalmos postraumático como una deformidad difícil de corregir. Están descritos abordajes cutáneos extensos como el coronal, el subciliar o el intraoral; la técnica descrita por Henry Kawamoto en 1998 describe un procedimiento de menor invasión mediante abordajes de mínima exposición que permiten la refracturación y el alineamiento del cuerpo zigomático, brindando así grandes ventajas sobre las técnicas convencionales. Presentamos el método aplicado en el servicio de Cirugía Reconstructiva del Centro Médico del Instituto de Seguridad Social del Estado de México y Municipios (ISSEMyM para el manejo de pacientes con enoftalmos como secuela de traumatismos del complejo cigomático-malar; para ello empleamos un método por asistencia con modelos estereolitográficos para la planificación quirúrgica del diseño de osteotomías para corregir la deformidad facial y para determinar las dimensiones del defecto a reparar, así como el tipo y cantidad de materiales aloplásticos o autólogos a emplear. Compartimos la experiencia de los casos tratados en nuestro Servicio entre 2006 y 2010.

  14. Hypertension: Believe it or not, a Complex Problem La hipertensión arterial: aunque no lo parezca, un problema complejo

    Directory of Open Access Journals (Sweden)

    Alfredo Darío Espinosa Brito

    2011-03-01

    Full Text Available High blood pressure (hypertension is recognized as a major health problem due both to its morbidity and the disability it causes and to its impact on mortality, especially cardiovascular mortality. However, effectively addressing its prevention and control, both in individuals and in the general population, does not seem to be an easy task, even these days. This paper aims to present different aspects of arterial hypertension (a concept through diagnosis, treatment and follow-up focusing on this entity as a complex system including multiple elements related to cardiovascular disease.La hipertensión arterial constituye un reconocido problema de salud, tanto por su morbilidad, por la discapacidad que provoca, como por su repercusión en la mortalidad, especialmente cardiovascular. Sin embargo, enfrentar eficazmente su prevención y control, tanto en los individuos como en la población en general, no parece una tarea fácil, aún en nuestros días. Este trabajo tiene como objetivo presentar diferentes aspectos de la hipertensión arterial (concepto pasando por el diagnóstico, tratamiento y seguimiento enfocando dicha entidad como un sistema complejo que abarca múltiples elementos relacionados con las enfermedades cardiovasculares.HYPERTENSION: BELIEVE IT OR NOT, A COMPLEX PROBLEMABSTRACTHigh blood pressure (hypertension is recognized as a major health problem due both to its morbidity and the disability it causes and to its impact on mortality, especially cardiovascular mortality. However, effectively addressing its prevention and control, both in individuals and in the general population, does not seem to be an easy task, even these days. This paper aims to present different aspects of arterial hypertension (a concept through diagnosis, treatment and follow-up focusing on this entity as a complex system including multiple elements related to cardiovascular disease.

  15. Role of genotype® mycobacterium common mycobacteria/additional species assay for rapid differentiation between Mycobacterium tuberculosis complex and different species of non-tuberculous mycobacteria

    Directory of Open Access Journals (Sweden)

    Amresh Kumar Singh

    2013-01-01

    Full Text Available Background: Mycobacterium tuberculosis complex (MTBC and non-tuberculous mycobacteria (NTM may or may not have same clinical presentations, but the treatment regimens are always different. Laboratory differentiation between MTBC and NTM by routine methods are time consuming and cumbersome to perform. We have evaluated the role of GenoType® Mycobacterium common mycobacteria/additional species (CM/AS assay for differentiation between MTBC and different species of NTM in clinical isolates from tuberculosis (TB cases. Materials and Methods: A total of 1080 clinical specimens were collected from January 2010 to June 2012. Diagnosis was performed by Ziehl-Neelsen staining followed by culture in BacT/ALERT 3D system (bioMerieux, France. A total of 219 culture positive clinical isolates (BacT/ALERT® MP cultures were selected for differentiation by p-nitrobenzoic acid (PNB sensitivity test as and BIO-LINE SD Ag MPT64 TB test considering as the gold standard test. Final identification and differentiation between MTBC and different species of NTM were further confirmed by GenoType® Mycobacterium CM/AS assay (Hain Lifescience, Nehren, Germany. Results: Out of 219 BacT/ALERT® MP culture positive isolates tested by PNB as 153 MTBC (69.9% and by GenoType® Mycobacterium CM/AS assay as 159 (72.6% MTBC and remaining 60 (27.4% were considered as NTM species. The GenoType® Mycobacterium CM/AS assay was proved 99.3% sensitive and 98.3% specific for rapid differentiation of MTBC and NTM. The most common NTM species were; Mycobacterium fortuitum 20 (33.3% among rapid growing mycobacteria and Mycobacterium intracellulare 11 (18.3% among slow growing mycobacteria. Conclusion: The GenoType® Mycobacterium assay makes rapid and accurate identification of NTM species as compared with different phenotypic and molecular diagnostic tool and helps in management of infections caused by different mycobacteria.

  16. Mycobacterium smegmatis infection of a prosthetic total knee arthroplasty.

    Science.gov (United States)

    Saffo, Zaid; Ognjan, Anthony

    2016-01-01

    The most common organisms causing prosthetic knee joint infections are staphylococci. However, arthroplasty infections with atypical microbial pathogens, such as Mycobacteria can occur. Due to the rarity of mycobacterial prosthetic joint infections, diagnosis, treatment, and management of these atypical infections represent a clinical challenge. A 71-year old female post-operative day 40 after a left total knee arthroplasty was hospitalized secondary to left knee pain and suspected arthroplasty infection. She had failed outpatient oral antimicrobial treatment for superficial stitch abscess; and outpatient IV/Oral antimicrobials for a clinical postoperative septic bursitis. Ultimately, resection arthroplasty with operative tissue acid fast bacterial cultures demonstrated growth of the Mycobacterium smegmatis group. Post-operatively, she completed a combination course of oral doxycycline and levofloxacin and successfully completed a replacement arthroplasty with clinical and microbial resolution of the infection. To our knowledge, literature review demonstrates three case of knee arthroplasty infection caused by the Mycobacterium smegmatis group. Correspondingly, optimal surgical procedures and antimicrobial management including antimicrobial selection, treatment duration are not well defined. Presently, the best treatment options consists of two step surgical management including prosthesis hardware removal followed by extended antimicrobial therapy, followed by consideration for re-implantation arthroplasty. Our case illustrates importance of considering atypical mycobacterial infections in post-operative arthroplasty infections not responding to traditional surgical manipulations and antimicrobials. For an arthroplasty infection involving the atypical Mycobacterium smegmatis group, two step arthroplasty revision, including arthroplasty resection, with a combination of oral doxycycline and levofloxacin can lead to successful infection resolution, allowing for a

  17. Human NOD2 Recognizes Structurally Unique Muramyl Dipeptides from Mycobacterium leprae.

    Science.gov (United States)

    Schenk, Mirjam; Mahapatra, Sebabrata; Le, Phuonganh; Kim, Hee Jin; Choi, Aaron W; Brennan, Patrick J; Belisle, John T; Modlin, Robert L

    2016-09-01

    The innate immune system recognizes microbial pathogens via pattern recognition receptors. One such receptor, NOD2, via recognition of muramyl dipeptide (MDP), triggers a distinct network of innate immune responses, including the production of interleukin-32 (IL-32), which leads to the differentiation of monocytes into dendritic cells (DC). NOD2 has been implicated in the pathogenesis of human leprosy, yet it is not clear whether Mycobacterium leprae, which has a distinct MDP structure, can activate this pathway. We investigated the effect of MDP structure on the innate immune response, finding that infection of monocytes with M. leprae induces IL-32 and DC differentiation in a NOD2-dependent manner. The presence of the proximal l-Ala instead of Gly in the common configuration of the peptide side chain of M. leprae did not affect recognition by NOD2 or cytokine production. Furthermore, amidation of the d-Glu residue did not alter NOD2 activation. These data provide experimental evidence that NOD2 recognizes naturally occurring structural variants of MDP. Copyright © 2016 Schenk et al.

  18. CD4 T Cell Dependent Colitis Exacerbation Following Re-Exposure of Mycobacterium avium ssp. paratuberculosis.

    Science.gov (United States)

    Suwandi, Abdulhadi; Bargen, Imke; Pils, Marina C; Krey, Martina; Zur Lage, Susanne; Singh, Anurag K; Basler, Tina; Falk, Christine S; Seidler, Ursula; Hornef, Mathias W; Goethe, Ralph; Weiss, Siegfried

    2017-01-01

    Mycobacterium avium ssp. paratuberculosis (MAP) is the causative agent of Johne's disease (JD), a chronic inflammatory bowel disease of cattle characterized by intermittent to chronic diarrhea. In addition, MAP has been isolated from Crohn's disease (CD) patients. The impact of MAP on severity of clinical symptoms in JD as well as its role in CD are yet unknown. We have previously shown that MAP is able to colonize inflamed enteric tissue and to exacerbate the inflammatory tissue response (Suwandi et al., 2014). In the present study, we analyzed how repeated MAP administration influences the course of dextran sulfate sodium (DSS)-induced colitis. In comparison to mice exposed to DSS or MAP only, repeated exposure of DSS-treated mice to MAP (DSS/MAP) revealed a significantly enhanced clinical score, reduction of colon length as well as severe CD4 + T cell infiltration into the colonic lamina propria . Functional analysis identified a critical role of CD4 + T cells in the MAP-induced disease exacerbation. Additionally, altered immune responses were observed when closely related mycobacteria species such as M. avium ssp. avium and M. avium ssp. hominissuis were administered. These data reveal the specific ability of MAP to aggravate intestinal inflammation and clinical symptoms. Overall, this phenotype is compatible with similar disease promoting capabilites of MAP in JD and CD.

  19. Microbe Profile: Mycobacterium tuberculosis: Humanity's deadly microbial foe.

    Science.gov (United States)

    Gordon, Stephen V; Parish, Tanya

    2018-04-01

    Mycobacterium tuberculosis is an expert and deadly pathogen, causing the disease tuberculosis (TB) in humans. It has several notable features: the ability to enter non-replicating states for long periods and cause latent infection; metabolic remodelling during chronic infection; a thick, waxy cell wall; slow growth rate in culture; and intrinsic drug resistance and antibiotic tolerance. As a pathogen, M. tuberculosis has a complex relationship with its host, is able to replicate inside macrophages, and expresses diverse immunomodulatory molecules. M. tuberculosis currently causes over 1.8 million deaths a year, making it the world's most deadly human pathogen.

  20. Mycobacterium marinum infection following contact with reptiles: vivarium granuloma.

    Science.gov (United States)

    Bouricha, Mehdi; Castan, Bernard; Duchene-Parisi, Elisabeth; Drancourt, Michel

    2014-04-01

    A 19-year-old man presented with a 1.5-cm nodule on the first dorsal metacarpal ray. The patient denied having contact with fish tanks or fish, but recalled handling many reptiles without gloves in the vivarium where he worked. A culture of a skin biopsy specimen yielded Mycobacterium marinum. The clinical outcome was favourable after a 2-week course of intramuscular gentamicin (180 mg daily) combined with a 6-week course of oral clarithromycin (500 mg twice a day). Doctors should be aware that vivariums, in addition to fish tanks, can be sources of M. marinum exposure. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

  1. Necrotizing Soft Tissue Infection Occurring after Exposure to Mycobacterium marinum

    Directory of Open Access Journals (Sweden)

    Shivani S. Patel

    2014-01-01

    Full Text Available Cutaneous infections caused by Mycobacterium marinum have been attributed to aquarium or fish exposure after a break in the skin barrier. In most instances, the upper limbs and fingers account for a majority of the infection sites. While previous cases of necrotizing soft tissue infections related to M. marinum have been documented, the importance of our presenting case is to illustrate the aggressive nature of M. marinum resulting in a persistent necrotizing soft tissue infection of a finger that required multiple aggressive wound debridements, followed by an amputation of the affected extremity, in order to hasten recovery.

  2. Mycobacterium avium complex disseminated infection in a kidney transplant recipient.

    Science.gov (United States)

    Fadlallah, J; Rammaert, B; Laurent, S; Lanternier, F; Pol, S; Franck, N; Mamzer, M F; Dupin, N; Lortholary, O

    2016-02-01

    Mycobacterium avium-intracellulare complex (MAC) infections are well known in immunocompromised patients, notably in human immunodeficiency virus infection, but remain scarcely described in kidney transplantation. Moreover, cutaneous involvement in this infection is very unusual. We describe here a disseminated infection caused by MAC in a kidney transplant recipient revealed by cutaneous lesions. This case highlights the need for an exhaustive, iterative microbiologic workup in the context of an atypical disease presentation in a renal transplant patient, regardless of the degree of immunosuppression. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  3. Improved method for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide.

    OpenAIRE

    Butler, W R; Kilburn, J O

    1982-01-01

    The acid medium required to test susceptibility of Mycobacterium tuberculosis to pyrazinamide (PZA) is a major problem in obtaining reliable test results. Satisfactory growth is usually obtained on Middlebrook and Cohn 7H10 medium at pH 5.5 if albumin-dextrose-catalase (ADC) supplement rather than oleic acid-albumin-dextrose-catalase is used; however, some lots of ADC supplement still fail to support growth at this low pH. A rapid turbidimetric test was developed to determine the growth-suppo...

  4. Anti-Mycobacterium tuberculosis activity of fungus Phomopsis stipata

    Directory of Open Access Journals (Sweden)

    Karina Andrade de Prince

    2012-03-01

    Full Text Available Our purpose was to determine the anti-Mycobacterium tuberculosis activity of the metabolites produced by the endophitic fungus Phomopsis stipata (Lib. B. Sutton, (Diaporthaceae, cultivated in different media. The antimycobacterial activity was assessed through the Resazurin Microtiter Assay (REMA and the cytotoxicity test performed on macrophage cell line. The extracts derived from fungi grown on Corn Medium and Potato Dextrose Broth presented the smallest values of Minimum Inhibitory Concentration (MIC and low cytotoxicity, which implies a high selectivity index. This is the first report on the chemical composition and antitubercular activity of metabolites of P. stipata, as well as the influence of culture medium on these properties.

  5. Mycobacterium goodii endocarditis following mitral valve ring annuloplasty.

    Science.gov (United States)

    Parikh, Rohan B; Grant, Matthew

    2017-03-21

    Mycobacterium goodii is an infrequent human pathogen which has been implicated in prosthesis related infections and penetrating injuries. It is often initially misidentified as a gram-positive rod by clinical microbiologic laboratories and should be considered in the differential diagnosis. We describe here the second reported case of M. goodii endocarditis. Species level identification was performed by 16S rDNA (ribosomal deoxyribonucleic acid) gene sequencing. The patient was successfully treated with mitral valve replacement and a prolonged combination of ciprofloxacin and trimethoprim/sulfamethoxazole. Confirmation of the diagnosis utilizing molecular techniques and drug susceptibility testing allowed for successful treatment of this prosthetic infection.

  6. Disseminated Mycobacterium celatum disease with prolonged pulmonary involvement

    DEFF Research Database (Denmark)

    Patsche, Cecilie Blenstrup; Svensson, Erik; Wejse, Christian

    2014-01-01

    Mycobacterium celatum is a rare cause of human infection, causing disseminated disease in immunosuppressed individuals. Infections localized to the lungs and the lymph nodes have also been reported in immunocompetent individuals. The existing literature on the subject is limited as are experiences...... with treatment regimens and durations. In the case presented herein, two different treatment regimens were applied to an immunocompromised HIV-negative patient with primary skin involvement and extensive pulmonary involvement due to suspected relapse on isoniazid, ethambutol, and clarithromycin treatment....... The treatment regimen was changed to azithromycin, ciprofloxacin, and pyrazinamide and the treatment duration was prolonged to a total of 24 months, with good effect....

  7. Deciphering the biology of Mycobacterium tuberculosis from thecomplete genome sequence

    DEFF Research Database (Denmark)

    Cole, S.T.; Krogh, Anders Stærmose

    1998-01-01

    Countless millions of people have died from tuberculosis, a chronic infectious disease caused by the tubercle bacillus. The complete genome sequence of the best-characterized strain of Mycobacterium tuberculosis, H37Rv, has been determined and analysed in order to improve our understanding....... tuberculosis differs radically from other bacteria in that a very large portion of its coding capacity is devoted to the production of enzymes involved in lipogenesis and lipolysis, and to two new families of glycine-rich proteins with a repetitive structure that may represent a source of antigenic variation....

  8. Genome-wide comparison of medieval and modern Mycobacterium leprae

    DEFF Research Database (Denmark)

    Schuenemann, Verena J; Singh, Pushpendra; Mendum, Thomas A

    2013-01-01

    Leprosy was endemic in Europe until the Middle Ages. Using DNA array capture, we have obtained genome sequences of Mycobacterium leprae from skeletons of five medieval leprosy cases from the United Kingdom, Sweden, and Denmark. In one case, the DNA was so well preserved that full de novo assembly...... origin for leprosy in the Americas, and the presence of an M. leprae genotype in medieval Europe now commonly associated with the Middle East. The exceptional preservation of M. leprae biomarkers, both DNA and mycolic acids, in ancient skeletons has major implications for palaeomicrobiology and human...

  9. Mistura com excesso enantiomérico de 50% (S75-R25 de bupivacaína complexada com ciclodextrinas e anestesia por via subaracnóidea em ratos Mezcla con exceso enantiomérico de 50% (S75-R25 de bupivacaina con complejo de ciclodextrinas y anestesia por vía subaracnoidea en ratones Complexation of 50% enantiomeric excess (S75-R25 bupivacaine with cyclodextrins and spinal block anesthesia in rats

    Directory of Open Access Journals (Sweden)

    Daniele Ribeiro de Araujo

    2006-10-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: Com a finalidade de prolongar a duração de ação e reduzir a toxicidade sistêmica, têm-se desenvolvido formulações de anestésicos locais (AL complexados com ciclodextrinas (CD. Este estudo realizou a caracterização físico-química e avaliou, em ratos, os efeitos dos complexos de inclusão de bupivacaína racêmica (S50-R50 e da mistura com excesso enantiomérico de 50% (S75-R25 de bupivacaína com hidroxipropil-betaciclodextrina (HP-beta-CD, comparando-os com as soluções atualmente utilizadas na clínica. MÉTODO: Os complexos de inclusão de S75-R25 em HP-beta-CD (razão molar 1:1 foram caracterizados por estudos de solubilidade de fases variando-se as concentrações de HP-beta-CD e a temperatura. Determinaram-se as constantes de afinidade (K pela HP-beta-CD e os parâmetros termodinâmicos para a complexação. Os bloqueios motor e sensitivo foram avaliados, por meio da administração subaracnóidea das formulações na concentração clínica de 0,5%. RESULTADOS: A formação de complexos de inclusão foi observada pelo aumento da solubilidade aquosa do AL sob diferentes temperaturas e concentrações de HP-beta-CD. Os testes in vivo mostraram que S50-R50HP-beta-CD e S75-R25HP-beta-CD reduziram a latência (p JUSTIFICATIVA Y OBJETIVOS: Con la finalidad de prolongar la duración de la acción y reducir la toxicidad sistémica, se han desarrollado formulaciones de AL con complejo de ciclodextrinas (CD. Ese estudio realizó la caracterización físico-química y evaluó en ratones, los efectos de los complejos de inclusión de bupivacaína racémica (S50-R50 y de la mezcla con exceso enantiomérico de 50% (S75-R25 de bupivacaína con hidroxipropil-betaciclodextrina (HP-beta-CD, comparándolos con las soluciones actualmente utilizadas en la clínica. MÉTODO: Los complejos de inclusión de S75-R25 en HP-beta-CD (razón molar 1:1 fueron caracterizados por estudios de solubilidad de fases variando las

  10. Mycobacterium tuberculosis Infection in a Domesticated Korean Wild Boar ( Sus scrofa coreanus).

    Science.gov (United States)

    Seo, Min-Goo; Ouh, In-Ohk; Kim, Munki; Lee, Jienny; Kim, Young-Hoan; Do, Jae-Cheul; Kwak, Dongmi

    2017-06-01

    Tuberculosis, a chronic progressive disease, has been reported in bovine, swine, and primate species. Here, we report the first case of Mycobacterium tuberculosis infection in a Korean wild boar ( Sus scrofa coreanus). The owners this domesticated boar brought it to the Gyeongbuk Veterinary Service Laboratory in Korea after it was found dead and severely emaciated. Demarcated yellowish white nodules were found around the larynx and retropharyngeal lymph node during necropsy. The lungs had diffuse fibrinous pleuritis, severe congestion, and scattered nodules. More nodules were found in the spleen. Tuberculosis is characterized by massive macrophage infiltration and central caseous necrosis; both characteristics were found in the lungs. Histopathologic examination revealed that the alveolar lumen had marked fibrosis and exudates. Examination of the fluid revealed extensive macrophage permeation. To confirm a Mycobacterium infection, PCR was performed using two primer sets specific to the rpoB gene of Mycobacterium; Mycobacterium was detected in the lungs and spleen. To identify the species of Mycobacterium, immunohistochemical evaluation was performed using antibodies against Mycobacterium tuberculosis and Mycobacterium bovis . The results revealed immunoreactivity against M. tuberculosis but not against M. bovis . The consumption of undercooked or raw meat from game animals may expose humans and other animals to sylvatic infection. Consequently, Koreans who ingest wild boar may be at risk of a tuberculosis infection. To reduce the risk of foodborne infection and maintain public health, continuous monitoring and control strategies are required.

  11. Repensando o ser enfermeiro docente na perspectiva do pensamento complexo Repensando el ser enfermeiro docente en la perspectiva del pensamiento complejo Rethinking the to be a nurse teacher in the perspective of the complex thought

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    Dirce Stein Backes

    2010-06-01

    Full Text Available Estudo qualitativo com características de pesquisa-ação. Objetivou-se, repensar o ser enfermeiro docente na perspectiva do pensamento complexo. Com a formação de uma comissão, em maio de 2009, responsável pelo desencadeamento das discussões acerca metodologias de ensino-aprendizagem, foi elaborado um projeto, com etapas seqüenciais de intervenção. Participaram do estudo, aproximadamente 380 discentes e 30 docentes do curso de enfermagem do Centro Universitário Franciscano. A análise temática das informações, obtidas por meio de encontros coletivos, possibilitou delimitar a categoria: Necessitando desenvolver a liderança e a habilidade didática para o ensino superior. A docência no ensino superior, não constitui um processo centrado na pessoa do professor, mas requer o envolvimento ativo e efetivo do estudante, como autor e protagonistas de sua própria história.Estudio cualitativo con características de pesquisa-acción. Se propuso pensar el ser enfermero docente en la perspectiva del pensamiento complejo. Con este objetivo, se constituyó, en mayo de 2009, una comisión, responsable por el desencadenamiento de discusiones sobre metodologías de enseñanza-aprendizaje. Fue elaborado un proyecto, con etapas secuenciales de intervención. Participaron del estudio alrededor de 380 estudiantes y 30 docentes del curso de Enfermería del "Centro Universitário Franciscano". El análisis temática de las informaciones, obtenidas por medio de encuentros colectivos, posibilitó delimitar la categoría: necesidad de desarrollar el liderazgo y la habilidad didáctica para la enseñanza superior. La docencia en la enseñanza superior no constituye un proceso centrado en la persona del profesor. Al contrario, requiere el involucramiento activo y efectivo del estudiante, como autor y protagonistas de su propia historia.Qualitative study with characteristics of research and action. The purpose was to consider the nurse teacher in the

  12. Description of Mycobacterium chelonae subsp. bovis subsp. nov., isolated from cattle (Bos taurus coreanae), emended description of Mycobacterium chelonae and creation of Mycobacterium chelonae subsp. chelonae subsp. nov.

    Science.gov (United States)

    Kim, Byoung-Jun; Kim, Ga-Na; Kim, Bo-Ram; Jeon, Che Ok; Jeong, Joseph; Lee, Seon Ho; Lim, Ji-Hun; Lee, Seung-Heon; Kim, Chang Ki; Kook, Yoon-Hoh; Kim, Bum-Joon

    2017-10-01

    Three rapidly growing mycobacterial strains, QIA-37 T , QIA-40 and QIA-41, were isolated from the lymph nodes of three separate Korean native cattle, Hanwoo (Bos taurus coreanae). These strains were previously shown to be phylogenetically distinct but closely related to Mycobacterium chelonae ATCC 35752 T by taxonomic approaches targeting three genes (16S rRNA, hsp6 and rpoB) and were further characterized using a polyphasic approach in this study. The 16S rRNA gene sequences of all three strains showed 99.7 % sequence similarity with that of the M. chelonae type strain. A multilocus sequence typing analysis targeting 10 housekeeping genes, including hsp65 and rpoB, revealed a phylogenetic cluster of these strains with M. chelonae. DNA-DNA hybridization values of 78.2 % between QIA-37 T and M. chelonae indicated that it belongs to M. chelonae but is a novel subspecies distinct from M. chelonae. Phylogenetic analysis based on whole-genome sequences revealed a 95.44±0.06 % average nucleotide identity (ANI) value with M. chelonae, slightly higher than the 95.0 % ANI criterion for determining a novel species. In addition, distinct phenotypic characteristics such as positive growth at 37 °C, at which temperature M. chelonae does not grow, further support the taxonomic status of these strains as representatives of a novel subspecies of M. chelonae. Therefore, we propose an emended description of Mycobacterium chelonae, and descriptions of M. chelonae subsp. chelonae subsp. nov. and M. chelonae subsp. bovis subsp. nov. are presented; strains ATCC 35752 T (=CCUG 47445 T =CIP 104535 T =DSM 43804 T =JCM 6388 T =NCTC 946 T ) and QIA-37 T (=KCTC 39630 T =JCM 30986 T ) are the type strains of the two novel subspecies.

  13. Complejo industrial de Beinasco, Italia

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    Levi, Corrado

    1966-02-01

    Full Text Available This establishment consists of the factory, the general offices and secondary services. They are located near Turin. The office building and general services has two basements, and three storeys. The basements are mainly taken up with garage space. The ground floor provides room for the technical offices, dressing rooms, washing rooms for workers and ancillaries. On the second floor the director and executives have their offices, and there is also a recreation room for the staff, and on the third floor are dining rooms for the staff and workers. This building has been vigorously designed, and is highly effective both in its formal and functional expressionism.Las edificaciones que lo componen son: la fábrica y las oficinas generales y servicios. Ha sido construido cerca de Turín. El edificio de las oficinas generales y servicios consta de dos sótanos, planta baja y dos plantas de altura. Los sótanos están ocupados principalmente por los garajes; la planta baja comprende las oficinas técnicas de la fábrica, vestuarios, lavabos de los operarios, etc.; la primera planta alberga las oficinas de la dirección y la administración, sala de recreo de los empleados, etc.; y en la segunda están el comedor de operarios y jefes, etc. Destaca este edificio por el vigor de su composición arquitectónica, por su plástica formal y por la sinceridad de expresión de los materiales empleados.

  14. par genes in Mycobacterium bovis and Mycobacterium smegmatis are arranged in an operon transcribed from "SigGC" promoters

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    Casart Yveth

    2008-03-01

    Full Text Available Abstract Background The ParA/Soj and ParB/Spo0J proteins, and the cis-acting parS site, participate actively in chromosome segregation and cell cycle progression. Genes homologous to parA and parB, and two putative parS copies, have been identified in the Mycobacterium bovis BCG and Mycobacterium smegmatis chromosomes. As in Mycobacterium tuberculosis, the parA and parB genes in these two non-pathogenic mycobacteria are located near the chromosomal origin of replication. The present work focused on the determination of the transcriptional organisation of the ~6 Kb orf60K-parB region of M. bovis BCG and M. smegmatis by primer extension, transcriptional fusions to the green fluorescence protein (GFP and quantitative RT-PCR. Results The parAB genes were arranged in an operon. However, we also found promoters upstream of each one of these genes. Seven putative promoter sequences were identified in the orf60K-parB region of M. bovis BCG, whilst four were identified in the homologous region of M. smegmatis, one upstream of each open reading frame (ORF. Real-time PCR assays showed that in M. smegmatis, mRNA-parA and mRNA-parB levels decreased between the exponential and stationary phases. In M. bovis BCG, mRNA-parA levels also decreased between the exponential and stationary phases. However, parB expression was higher than parA expression and remained almost unchanged along the growth curve. Conclusion The majority of the proposed promoter regions had features characteristic of Mycobacterium promoters previously denoted as Group D. The -10 hexamer of a strong E. coli σ70-like promoter, located upstream of gidB of M. bovis BCG, overlapped with a putative parS sequence, suggesting that the transcription from this promoter might be regulated by the binding of ParB to parS.

  15. Mycobacterium alsense sp. nov., a scotochromogenic slow grower isolated from clinical respiratory specimens

    DEFF Research Database (Denmark)

    Tortoli, Enrico; Richter, Elvira; Borroni, Emanuele

    2016-01-01

    . Mycobacterium asiaticum is the most closely related species on the basis of the 16S rRNA sequence (similarity 99.3%); the average nucleotide Identity between the genomes of the two species is 80.72%, clearly below the suggested cutoff (95-96%). The name M. alsense is proposed here for the new species......"Mycobacterium alsiense", although reported in 2007, has not been validly published so far. The polyphasic characterization of the three strains available so far led us to the conclusion that they represent a distinct species within the genus Mycobacterium. The proposed new species grows slowly...

  16. Sporotrichoid-Like Spread of Cutaneous Mycobacterium chelonae in an Immunocompromised Patient

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    Daria Marley Kemp

    2017-01-01

    Full Text Available Mycobacterium chelonae is a rapidly growing mycobacterium found in water and soil that can cause local cutaneous infections in immunocompetent hosts but more frequently affects immunocompromised patients. Typically, patients will present with painful subcutaneous nodules of the joints or soft tissues from traumatic inoculation. However, exhibiting a sporotrichoid-like pattern of these nodules is uncommon. Herein, we report a case of sporotrichoid-like distribution of cutaneous Mycobacterium chelonae in a patient with systemic lupus erythematosus on significant immunosuppressive medications. Clinicians treating immunocompromised patients should be cognizant of their propensity to develop unusual infections and atypical presentations.

  17. Relación del pulso hidrológico con la estratigrafía del sedimento del complejo de humedales de Ayapel, Colombia

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    Alex Rúa

    2014-01-01

    Full Text Available El pulso hidrológico y la transferencia de masa entre los tributarios y humedales conectan los ambientes terrestres con los reotróficos. La interacción agua- sedimento en humedales de la sabana atlántica colombiana es prácticamente desconocida a escala multidecadal. Por esta razón, se realizaron tres correlaciones estratigráficas entre nueve perfiles de sedimento (13-30 cm del complejo de humedales de Ayapel (CHA que abarcaron ca. 110-600 a BP. Las correlaciones enmarcaron (i zonas de alta presión antrópica, (ii hidrodinámica principal y (iii aportes de materia orgánica (MO. El CHA se extiende 150 km2 de los cuales >84 % comprendió sedimento mineral óxico susceptible de diagénesis temprana. En contraste, el sedimento reducido en estratos anaerobios de algunos humedales menores indicó metanogénesis. La intensidad del pulso hidrológico se relacionó con el registro estratigráfico del rizoma de Eichhornia crassipes. Arena y grava sedimentaron bajo flujo turbulento cerca del tributario Quebradona, mientras el limo se cementó con arcilla en la zona léntica o con arena en caños de conexión. El viento sobre la lámina de agua somera favoreció la resuspensión y transporte del sedimento proveniente de los tributarios. Por esta razón, se propusieron tasas netas de sedimentación relativamente lentas en la boca de Quebradona (ca. 0,15 cm a-1, caños (ca. 0,1 cm a-1 y humedales temporales (ca. 0,05 cm a-1. Además se constató que el sedimento del CHA exportó los productos de degradación de MO a la cuenca baja del Río San Jorge y la atmósfera.

  18. EVALUACIÓN DE LA ROTIFEROFAUNA PRESENTE EN EL COMPLEJO DE PAJARALES DURANTE LA ÉPOCA LLUVIOSA, DEPARTAMENTO DE MAGDALENA, COLOMBIA

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    SEBASTIÁN CELIS

    2008-05-01

    Full Text Available Con miras a evaluar posibles efectos de la recomunicación del río Magdalena con su antiguo delta, se determinó la abundancia y composición de la rotiferofauna del Complejo de Pajarales (CP, durante el período de mayor abundancia anual de zooplancton. El phylum Rotifera fue seleccionado por sus altas tasas reproductivas y eficiencia en procesos de transformación energética. Las muestras se colectaron con botella van Dorn durante la segunda temporada lluviosa de 2006. En cada una de las cuatro estaciones analizadas se registraron la salinidad, temperatura, pH y oxígeno disuelto. La diversidad se calculó empleando el índice de Shannon-Wiener H’ (log10 comparando estaciones y fechas de muestreo para establecer variaciones en el período de estudio. Gráficamente se relacionaron variables fisicoquímicas con valores de diversidad que emplearon un α de 0.05 y 95% de intervalo de confianza. En total, fueron encontrados 20 morfotipos pertenecientes a las familias Brachionidae, Lecanidae, Filiniidae, Synchaetidae, Hexarthriidae y Testudinellidae, siendo Brachionidae y Lecanidae las más abundantes. La relación entre diversidad y variables fisicoquímicas, indica que la salinidad es la principal responsable de la diversidad de rotíferos. En conclusión, la rotiferofauna actual en el CP es más abundante y diversa que hace 16 años, antes de la recomunicación con el río Magdalena. Así mismo, la concentración de oxigeno disuelto, pH y salinidad son diferentes. Teniendo en cuenta que la salinidad es el factor que más influyó en la diversidad de rotíferos, la reapertura de canales probablemente favoreció el incremento en la diversidad de rotíferos en el CP.

  19. Papel del calcio en la modulación de la supervivencia intracelular de Mycobacterium tuberculosis

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    Luis Fernando García

    2001-04-01

    Full Text Available

    Mycobacterium tuberculosis ( Mtb induce alteraciones en la señalización que podrían favorecer el crecimiento intracelular de la bacteria. Hay alteraciones en respuestas al IFNγ, activación de fosfatasas, inhibición del fagolisosoma y cambios en los flujos
    de iones como el Ca2+. Estos eventos dependen de los receptores por los cuales sea reconocida la micobacteria, por ejemplo, en macrófagos murinos la interacción con el receptor de manosa (MMR, pero no con CD14, ni receptores tipo “scavenger” induce
    flujos de Ca2+, que alteran la mitocondria y se asocian con apoptosis. En macrófagos humanos, la fagocitosis a través de receptores Fc, pero no de receptores de complemento, aumenta el Ca2+ que favorece la translocación al fagosoma de la calmodulina (CaM que activa la kinasa II dependiente de CaM (CaMKII, permitiendo la fusión del fagolisosoma y disminuyendo el crecimiento de Mtb. El bloqueo de esta vía favorece el crecimiento de la micobacteria. Esto indica que el Ca2+ puede modular la apoptosis y las señales que controlan la micobacteria, sugiriendo que la modulación de esta vía puede ser un blanco putativo para la intervención farmacológica. Por lo tanto proponemos como objetivo general establecer si la terapia anti-micobacteriana modula las vías de señalización dependientes de Ca2+ y la actividad de la CaMKII en macrófagos infectados con Mtb.

     

     

  20. Uracil excision repair in Mycobacterium tuberculosis cell-free extracts.

    Science.gov (United States)

    Kumar, Pradeep; Bharti, Sanjay Kumar; Varshney, Umesh

    2011-05-01

    Uracil excision repair is ubiquitous in all domains of life and initiated by uracil DNA glycosylases (UDGs) which excise the promutagenic base, uracil, from DNA to leave behind an abasic site (AP-site). Repair of the resulting AP-sites requires an AP-endonuclease, a DNA polymerase, and a DNA ligase whose combined activities result in either short-patch or long-patch repair. Mycobacterium tuberculosis, the causative agent of tuberculosis, has an increased risk of accumulating uracils because of its G + C-rich genome, and its niche inside host macrophages where it is exposed to reactive nitrogen and oxygen species, two major causes of cytosine deamination (to uracil) in DNA. In vitro assays to study DNA repair in this important human pathogen are limited. To study uracil excision repair in mycobacteria, we have established assay conditions using cell-free extracts of M. tuberculosis and M. smegmatis (a fast-growing mycobacterium) and oligomer or plasmid DNA substrates. We show that in mycobacteria, uracil excision repair is completed primarily via long-patch repair. In addition, we show that M. tuberculosis UdgB, a newly characterized family 5 UDG, substitutes for the highly conserved family 1 UDG, Ung, thereby suggesting that UdgB might function as backup enzyme for uracil excision repair in mycobacteria. Copyright © 2011 Elsevier Ltd. All rights reserved.

  1. The Complete Structure of the Mycobacterium smegmatis 70S Ribosome.

    Science.gov (United States)

    Hentschel, Jendrik; Burnside, Chloe; Mignot, Ingrid; Leibundgut, Marc; Boehringer, Daniel; Ban, Nenad

    2017-07-05

    The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-Å cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics. Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.

  2. An outbreak of Mycobacterium fortuitum cutaneous infection associated with mesotherapy.

    Science.gov (United States)

    Quiñones, C; Ramalle-Gómara, E; Perucha, M; Lezaun, M-E; Fernández-Vilariño, E; García-Morrás, P; Simal, G

    2010-05-01

    We describe an outbreak of Mycobacterium fortuitum cutaneous infections associated with mesotherapy in La Rioja, Spain. Descriptive epidemiology. Private practice. Case subjects were customers of a single beauty salon who were treated with mesotherapy injections. Two skin biopsies were taken from each patient. Over the designated period, 138 women received mesotherapy. Of these women, 39, or 28.3%, developed lesions ultimately thought to be caused by Mycobacterium fortuitum infection. The number of lesions per patient varied from 3 to 20 in the most severe case. Most of the lesions were indurated, erythematous or violaceous papules, some progressing to become fluctuant boils with suppuration, fistulization and scarring. The individual lesions varied in diameter from 0.5 to 6 cm. Two patients (5.1%) developed inguinal or axillary adenopathy. Two others presented with fever. One reported muscular pain. In 12 of the 39 cases, M. fortuitum was isolated from the wound cultures. The patients were all successfully treated with clarithromycin and levofloxacin. We identified a large outbreak of rapidly growing mycobacterial lesions among women who received mesotherapy injections in a single beauty salon.

  3. Circumvention of the Mycobactin Requirement of Mycobacterium paratuberculosis

    Science.gov (United States)

    Morrison, Norman E.

    1965-01-01

    Morrison, Norman E. (Johns Hopkins University-Leonard Wood Memorial Leprosy Research Laboratory, Baltimore, Md.). Circumvention of the mycobactin requirement of Mycobacterium paratuberculosis. J. Bacteriol. 89:762–767. 1965.—The mycobactin growth requirement of Mycobacterium paratuberculosis was circumvented on glucose-containing synthetic medium with an initial pH of 5.5. Mycobactin was required during the first transfer on the synthetic medium. Subsequent transfers have grown in the absence of mycobactin. The growth of mycobactin-“independent” strains of M. paratuberculosis on the synthetic medium was found to be stimulated by low concentrations of mycobactin. The circumvention of the mycobactin requirement appears to depend upon the properties of the medium and not upon having created conditions which promote endogenous mycobactin synthesis. Investigation of the glucose-containing synthetic medium showed that: (i) growth stimulatory compounds were formed during autoclaving, and (ii) compared with neutrality a pH of 5.5 gave markedly increased pellicle yields. It was suggested that the growth-stimulatory compounds formed during autoclaving may in part be responsible for the circumvention of the mycobactin requirement. PMID:14273658

  4. Structural Implications of Mutations Conferring Rifampin Resistance in Mycobacterium leprae.

    Science.gov (United States)

    Vedithi, Sundeep Chaitanya; Malhotra, Sony; Das, Madhusmita; Daniel, Sheela; Kishore, Nanda; George, Anuja; Arumugam, Shantha; Rajan, Lakshmi; Ebenezer, Mannam; Ascher, David B; Arnold, Eddy; Blundell, Tom L

    2018-03-22

    The rpoB gene encodes the β subunit of RNA polymerase holoenzyme in Mycobacterium leprae (M. leprae). Missense mutations in the rpoB gene were identified as etiological factors for rifampin resistance in leprosy. In the present study, we identified mutations corresponding to rifampin resistance in relapsed leprosy cases from three hospitals in southern India which treat leprosy patients. DNA was extracted from skin biopsies of 35 relapse/multidrug therapy non-respondent leprosy cases, and PCR was performed to amplify the 276 bp rifampin resistance-determining region of the rpoB gene. PCR products were sequenced, and mutations were identified in four out of the 35 cases at codon positions D441Y, D441V, S437L and H476R. The structural and functional effects of these mutations were assessed in the context of three-dimensional comparative models of wild-type and mutant M. leprae RNA polymerase holoenzyme (RNAP), based on the recently solved crystal structures of RNAP of Mycobacterium tuberculosis, containing a synthetic nucleic acid scaffold and rifampin. The resistance mutations were observed to alter the hydrogen-bonding and hydrophobic interactions of rifampin and the 5' ribonucleotide of the growing RNA transcript. This study demonstrates that rifampin-resistant strains of M. leprae among leprosy patients in southern India are likely to arise from mutations that affect the drug-binding site and stability of RNAP.

  5. Subversion of Schwann Cell Glucose Metabolism by Mycobacterium leprae*

    Science.gov (United States)

    Medeiros, Rychelle Clayde Affonso; Girardi, Karina do Carmo de Vasconcelos; Cardoso, Fernanda Karlla Luz; Mietto, Bruno de Siqueira; Pinto, Thiago Gomes de Toledo; Gomez, Lilian Sales; Rodrigues, Luciana Silva; Gandini, Mariana; Amaral, Julio Jablonski; Antunes, Sérgio Luiz Gomes; Corte-Real, Suzana; Rosa, Patricia Sammarco; Pessolani, Maria Cristina Vidal; Nery, José Augusto da Costa; Sarno, Euzenir Nunes; Batista-Silva, Leonardo Ribeiro; Sola-Penna, Mauro; Oliveira, Marcus Fernandes; Moraes, Milton Ozório; Lara, Flavio Alves

    2016-01-01

    Mycobacterium leprae, the intracellular etiological agent of leprosy, infects Schwann promoting irreversible physical disabilities and deformities. These cells are responsible for myelination and maintenance of axonal energy metabolism through export of metabolites, such as lactate and pyruvate. In the present work, we observed that infected Schwann cells increase glucose uptake with a concomitant increase in glucose-6-phosphate dehydrogenase (G6PDH) activity, the key enzyme of the oxidative pentose pathway. We also observed a mitochondria shutdown in infected cells and mitochondrial swelling in pure neural leprosy nerves. The classic Warburg effect described in macrophages infected by Mycobacterium avium was not observed in our model, which presented a drastic reduction in lactate generation and release by infected Schwann cells. This effect was followed by a decrease in lactate dehydrogenase isoform M (LDH-M) activity and an increase in cellular protection against hydrogen peroxide insult in a pentose phosphate pathway and GSH-dependent manner. M. leprae infection success was also dependent of the glutathione antioxidant system and its main reducing power source, the pentose pathway, as demonstrated by a 50 and 70% drop in intracellular viability after treatment with the GSH synthesis inhibitor buthionine sulfoximine, and aminonicotinamide (6-ANAM), an inhibitor of G6PDH 6-ANAM, respectively. We concluded that M. leprae could modulate host cell glucose metabolism to increase the cellular reducing power generation, facilitating glutathione regeneration and consequently free-radical control. The impact of this regulation in leprosy neuropathy is discussed. PMID:27555322

  6. Infection by Mycobacterium bovis in a dog from Brazil

    Directory of Open Access Journals (Sweden)

    Vivianne Cambuí Figueiredo Rocha

    Full Text Available Abstract Tuberculosis (TB is a chronic disease caused by bacteria belonging to the Mycobacterium tuberculosis complex (MtbC. This disease rarely affects dogs. Canine infections are usually caused by M. tuberculosis. Mycobacterium bovis infections are rare in dogs and associated with consumption of raw milk or contaminated products. Here, we report a Boxer dog who had a M. bovis infection and was admitted to a Brazilian veterinary hospital with a presumptive diagnosis of chronic ehrlichiosis. Despite receiving treatment for chronic ehrlichiosis, it progressed to death. TB was diagnosed during post-mortem examinations using histopathological analysis. Ziehl-Neelsen staining revealed acid-fast bacilli in the kidneys, liver, mesentery, and a mass adhered to the liver. Further, PCR-restriction analysis was performed to identify mycobacteria in the samples. A restriction profile compatible with MtbC was found in the lungs. In addition, PCR-based MtbC typing deletions at different loci of chromosome 9 enabled the identification of M. bovis in the lungs. Therefore, it is very essential to perform differential diagnosis of TB in dogs with non-specific clinical signs and who do not respond to treatment, particularly those who had been in contact with TB-infected cattle or owners. Further, we highlight the use of molecular methods for the identification of bacilli, improving the diagnosis and aiding epidemiological studies.

  7. Ready Experimental Translocation of Mycobacterium canettii Yields Pulmonary Tuberculosis.

    Science.gov (United States)

    Bouzid, Fériel; Brégeon, Fabienne; Lepidi, Hubert; Donoghue, Helen D; Minnikin, David E; Drancourt, Michel

    2017-12-01

    Mycobacterium canettii , which has a smooth colony morphology, is the tuberculous organism retaining the most genetic traits from the putative last common ancestor of the rough-morphology Mycobacterium tuberculosis complex. To explore whether M. canettii can infect individuals by the oral route, mice were fed phosphate-buffered saline or 10 6 M. canettii mycobacteria and sacrificed over a 28-day experiment. While no M. canettii was detected in negative controls, M. canettii -infected mice yielded granuloma-like lesions for 4/4 lungs at days 14 and 28 postinoculation (p.i.) and positive PCR detection of M. canettii for 5/8 mesenteric lymph nodes at days 1 and 3 p.i. and 5/6 pooled stools collected from day 1 to day 28 p.i. Smooth M. canettii colonies grew from 68% of lungs and 36% of spleens and cervical lymph nodes but fewer than 20% of axillary lymph nodes, livers, brown fat samples, kidneys, or blood samples throughout the 28-day experiment. Ready translocation in mice after digestive tract challenge demonstrates the potential of ingested M. canettii organisms to relocate to distant organs and lungs. The demonstration of this relocation supports the possibility that populations may be infected by environmental M. canettii . Copyright © 2017 American Society for Microbiology.

  8. Molecular characterisation of Mycobacterium caprae strains isolated in Poland.

    Science.gov (United States)

    Krajewska-Wędzina, Monika; Kozińska, Monika; Orłowska, Blanka; Weiner, Marcin; Szulowski, Krzysztof; Augustynowicz-Kopeć, Ewa; Anusz, Krzysztof; Smith, Noel H

    2018-03-10

    Bovine tuberculosis (bovine TB, bTB) is caused by bovine bacilli: Mycobacterium bovis and M caprae The studies conducted in Poland, in the National Bovine Tuberculosis Reference Laboratory in the Department of Microbiology of the National Veterinary Research Institute in Pulawy, show that animal tuberculosis in Poland is also caused by M caprae We here describe the identification and genotypic assessment of 52 isolates of M caprae obtained from Polish cattle and wild animals over the last five years. We show that strains isolated from bison have significant genotypic diversity and are distinct compared with the genotypes of strains isolated from cattle. Similarly, isolates from cattle herds can be highly genotypically variable. Formal designation of the members of the Mycobacterium tuberculosis complex is controversial in Poland; there is a gap in veterinary legislation with regard to bTB and no explicit mention of M caprae causing tuberculosis in animal. © British Veterinary Association (unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  9. Antibacterial Activity of Medicinal Aqueous Plant Extracts against Mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Muna Mohammed Buzayan

    2012-09-01

    Full Text Available Tuberculosis (TB remains a serious health problem in many regions of the world, and the development of resistance to antibiotics by this microbe created the need for new drugs to replace those which have lost effectiveness. This study assesses the medicinal anti-Mycobacterium tuberculosis properties of natural products obtained from plants collected from Eastern Libya. In this study aqueous extracts of nine different plants were assayed for their Mycobacterium tuberculosis inhibitory activity using the BACTEC MGIT960 susceptibility test method. The aqueous extracts of Ceratonia siliqua L, Helichrysum stoechas (L. Moench and Thymus algeriensis did not show any activity against M. tuberculosis in different concentrations. The aqueous extract of Marrubium vulgare L. from Syria showed high activity against M. tuberculosis. Marrubium alysson L., Marrubium vulgare L., Pistacia lentiscus L, Quercus coccifera L, Thymus capitatus (L. Hoffm. & Link, showed varying degrees of activity against M. tuberculosis. The results of this study show that aqueous extracts from six different medicinal plants have different effects against M. tuberculosis in vitro.

  10. Rapid drug susceptibility test of mycobacterium tuberculosis by bioluminescence sensor

    Science.gov (United States)

    Lu, Bin; Xu, Shunqing; Chen, Zifei; Zhou, Yikai

    2001-09-01

    With the persisting increase of drug-resistant stains of M. Tuberculosis around the world, rapid and sensitive detection of antibiotic of M. Tuberculosis is becoming more and more important. In the present study, drug susceptibility of M. tuberculosis were detected by recombination mycobacteriophage combined with bioluminescence sensor. It is based on the use of recombination mycobacteriophage which can express firefly luciferase when it infects viable mycobacteria, and can effectively produce quantifiable photon. Meanwhile, in mycobacterium cells treated with active antibiotic, no light is observed. The emitted light is recorded by a bioluminscence sensor, so the result of drug-resistant test can be determined by the naked eye. 159 stains of M. tuberculosis were applied to this test on their resistant to rifampin, streptomycin and isoniazid. It is found that the agreement of this assay with Liewenstein- Jensen slat is: rifampin 95.60 percent, isoniazid 91.82 percent, streptomycin 88.68 percent, which showed that it is a fast and practical method to scene and detect drug resistant of mycobacterium stains.

  11. Identification of new genomospecies in the Mycobacterium terrae complex.

    Directory of Open Access Journals (Sweden)

    Yun Fong Ngeow

    Full Text Available Members of the Mycobacterium terrae complex are slow-growing, non-chromogenic acid-fast bacilli found in the natural environment and occasionally in clinical material. These genetically closely-related members are difficult to differentiate by conventional phenotypic and molecular tests. In this paper we describe the use of whole genome data for the identification of four strains genetically similar to Mycobacterium sp. JDM601, a newly identified member of the M. terrae complex. Phylogenetic information from the alignment of genome-wide orthologous genes and single nucleotide polymorphisms show consistent clustering of the four strains together with M. sp. JDM601 into a distinct clade separate from other rapid and slow growing mycobacterial species. More detailed inter-strain comparisons using average nucleotide identity, tetra-nucleotide frequencies and analysis of synteny indicate that our strains are closely related to but not of the same species as M. sp. JDM601. Besides the 16S rRNA signature described previously for the M. terrae complex, five more hypothetical proteins were found that are potentially useful for the rapid identification of mycobacterial species belonging to the M. terrae complex. This paper illustrates the versatile utilization of whole genome data for the delineation of new bacterial species and introduces four new genomospecies to add to current members in the M. terrae complex.

  12. Geomorfología y sedimentología de los ambientes depositacionales recientes del complejo estuarino de los ríos Hueque y Curarí, Estado Falcón, Venezuela

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    Scarlet Cartaya

    2012-02-01

    Full Text Available En este trabajo se presentan los resultados de la caracterización geomorfológica y sedimentológica del complejo estuarino de la desembocadura de los ríos Hueque y Curarí, localizada en la costa nororiental del Estado Falcón (Venezuela. La investigación se desarrolló en tres fases. En la fase de campo se recolectaron muestras de sedimentos y se realizaron mediciones morfométricas de los ambientes depositacionales. En la fase de laboratorio se ejecutaron análisis sedimentológicos de textura, carbonatos, materia orgánica, minerales pesados y mineralogía por difracción de rayos X. La fase de gabinete se basó en la interpretación y análisis espacial de los ambientes depositacionales con apoyo en fotografías aéreas y mapas topográficos a escala 1:25 000; en la confección de los mapas y perfiles del área, y en la elaboración del modelo sedimentológico. La desembocadura de los ríos Hueque y Curarí se encuentra en un borde costero de clima semi-árido, con una cuenca de baja densidad de drenaje y dinámica litoral de rango micromareal, marea mixta, con oleaje de alta energía y corriente litoral favorable. Se identificaron dos grandes conjuntos sedimentológicos: Complejo Cordón Litoral (infraplaya, mesoplaya, supraplaya, dunas playeras, contrabarrera y barra, de litofacies arenosas y el Complejo Pantanoso (cauces de los ríos Hueque y Curarí, canal de marea y caño de marea, de litofacies arcillo-limosa. Los canales fluviales se comportan como estuarios hipersalinos.

  13. Catálisis Asimétrica con complejos de rodio e iridio. Aplicación en síntesis de compuestos biológicamente activos

    OpenAIRE

    Cristóbal Lecina, Edgar

    2014-01-01

    La hidrogenación catalítica asimétrica es uno de los métodos más importantes en síntesis orgánica. Este proceso permite acceder a importantes productos quirales a escala multigramo utilizando hidrógeno (que es barato) y pequeñas cantidades de catalizador. Dichas hidrogenaciones requieren el empleo de complejos metálicos que contienen ligandos quirales y se presentan como un método muy atractivo desde el punto de vista de la economía atómica. Hasta el momento, se han descrito una amplia varied...

  14. Prevalencia de traumatismos dentoalveolares en pacientes infantiles del complejo asistencial Dr. Sótero del Río Prevalence of dental trauma of infants attended at Dr. Sótero del Río Hospital

    OpenAIRE

    PF Castro Brezzo; E Dreyer Arroyo

    2012-01-01

    En el presente estudio descriptivo de incidencia, 604 niños menores de 15 años fueron examinados y atendidos en la Clínica de Odontopediatría del Complejo Asistencial Dr. Sotero del Río, con diagnóstico de Traumatismo Dentoalveolar (TDA), desde Enero a Diciembre del 2010. El propósito de este estudio fue caracterizar los TDA de acuerdo a la clasificación de Andreasen y Andreasen, determinar su frecuencia y distribución estacional, tipo y número de dientes afectados, distribución de género y e...

  15. Síntesis y caracterización de un cuadrado supramoleculara partir del auto-ensamblaje del complejo[1,2-bis(difenilfosfino)etano] bistrifluorometanosulfonatopaladio (ii) y 4,4´-bipiridina

    OpenAIRE

    Posada, Julian; Avella, Eliseo; Duarte, Alvaro; Echegoyen, Luis

    2011-01-01

    Por primera vez se desarrolló la síntesisy caracterización (IR, UV, RMN-1H,31P, 19F) de un nuevo cuadrado supramolecular[3] mediante el auto-ensamblajedel complejo [Pd(CF3SO3)2(Dppe)] [1] yel ligando orgánico 4,4´-Bipiridina [2].Se evidenció la obtención de [3] en mezclacon otra especie supramolecular [4],asignada como un macrociclo triangular.Las estructuras de [3] y [4] se elucidaroncon base en los desplazamientos químicos,la proporcionalidad entre sus integralesy en los patrones de acoplam...

  16. SÍNTESIS Y CARACTERIZACIÓN DE UN CUADRADO SUPRAMOLECULARA PARTIR DEL AUTO-ENSAMBLAJE DEL COMPLEJO[1,2-BIS(DIFENILFOSFINO)ETANO] BISTRIFLUOROMETANOSULFONATOPALADIO (II) Y 4,4´-BIPIRIDINA

    OpenAIRE

    Julian Posada; Eliseo Avella; Alvaro Duarte; Luis Echegoyen

    2011-01-01

    Por primera vez se desarrolló la síntesisy caracterización (IR, UV, RMN-1H,31P, 19F) de un nuevo cuadrado supramolecular[3] mediante el auto-ensamblajedel complejo [Pd(CF3SO3)2(Dppe)] [1] yel ligando orgánico 4,4´-Bipiridina [2].Se evidenció la obtención de [3] en mezclacon otra especie supramolecular [4],asignada como un macrociclo triangular.Las estructuras de [3] y [4] se elucidaroncon base en los desplazamientos químicos,la proporcionalidad entre sus integralesy en los patrones de acoplam...

  17. La Enseñanza y el Aprendizaje de los Números Complejos: un Estudio en el Nivel Universitario (Teaching and Learning of Complex Numbers: A Study at University Level

    Directory of Open Access Journals (Sweden)

    Tomás Pardo

    2007-09-01

    Full Text Available Presentamos algunos de los resultados más relevantes de un estudio sobre la problemática de la enseñanza y el aprendizaje de los números complejos. El estudio se ha dirigido a recabar información para sustentar sugerencias de intervención en las pautas educativas en relación con esta temática. We present some of the most relevant results of a study concerning the teaching and learning of complex numbers. The study is focused on collecting data to support suggestions for teaching interventions related to this content.

  18. Reconstrucción del complejo areola-pezón: revisión de 60 casos Nipple-areola complex reconstruction: revision of 60 cases

    Directory of Open Access Journals (Sweden)

    R. Fernández García

    2007-06-01

    Full Text Available La creación del complejo areola-pezón, CAP, constituye el último tiempo de la reconstrucción mamaria, transformando la reconstrucción del montículo mamario en una mama. Debemos considerar pues la reconstrucción de la areola y el pezón, como la culminación de la reconstrucción de la mama. Existen numerosas técnicas descritas para la reconstrucción del CAP. El objetivo de este estudio es determinar el grado de satisfacción psicológica de las pacientes tras la misma. El presente estudio fue diseñado como una revisión clínica retrospectiva de 60 pacientes. Tras la revisión de las historias clínicas, las pacientes fueron entrevistadas procediéndose a la realización del cuestionario. El aspecto a cambiar más deseado fue la falta/pérdida de proyección del pezón. No obstante, el 22% de las pacientes respondieron que no cambiarían nada acerca de su reconstrucción. La satisfacción acerca de la reconstrucción del montículo mamario fue excelente o buena para el 68%, normal para el 23% y pobre para el 9%. En cambio, para la reconstrucción del complejo areola-pezón fue excelente o buena para el 50%, normal para el 45% y pobre para el 5%. No se encontraron diferencias significativas entre las diferentes técnicas en función del tiempo transcurrido entre el momento de la mastectomía y el tercer tiempo de la reconstrucción (p=0,06. La técnica de la donación contralateral de pezón fue la que ofreció una mayor satisfacción (2,67 puntos y proyección (7,23 puntos. A pesar de las diferencias en sus medias, no se demostró ninguna diferencia estadísticamente significativa. Teniéndo en cuenta la técnica usada para la reconstrucción de la areola, la técnica de la donación-injerto de piel inguinal fue la que ofreció mayor satisfacción (3 puntos y coloración (8,57 puntos. El estudio estadístico objetivó diferencias estadísticamente significativas (p=0,01. Entre las distintas técnicas de reconstrucción del pezón no

  19. Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera.

    Science.gov (United States)

    Gupta, Radhey S; Lo, Brian; Son, Jeen

    2018-01-01

    The genus Mycobacterium contains 188 species including several major human pathogens as well as numerous other environmental species. We report here comprehensive phylogenomics and comparative genomic analyses on 150 genomes of Mycobacterium species to understand their interrelationships. Phylogenetic trees were constructed for the 150 species based on 1941 core proteins for the genus Mycobacterium , 136 core proteins for the phylum Actinobacteria and 8 other conserved proteins. Additionally, the overall genome similarity amongst the Mycobacterium species was determined based on average amino acid identity of the conserved protein families. The results from these analyses consistently support the existence of five distinct monophyletic groups within the genus Mycobacterium at the highest level, which are designated as the " Tuberculosis-Simiae ," " Terrae," " Triviale ," " Fortuitum-Vaccae ," and " Abscessus-Chelonae " clades. Some of these clades have also been observed in earlier phylogenetic studies. Of these clades, the " Abscessus-Chelonae" clade forms the deepest branching lineage and does not form a monophyletic grouping with the " Fortuitum-Vaccae " clade of fast-growing species. In parallel, our comparative analyses of proteins from mycobacterial genomes have identified 172 molecular signatures in the form of conserved signature indels and conserved signature proteins, which are uniquely shared by either all Mycobacterium species or by members of the five identified clades. The identified molecular signatures (or synapomorphies) provide strong independent evidence for the monophyly of the genus Mycobacterium and the five described clades and they provide reliable means for the demarcation of these clades and for their diagnostics. Based on the results of our comprehensive phylogenomic analyses and numerous identified molecular signatures, which consistently and strongly support the division of known mycobacterial species into the five described clades, we

  20. Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

    Science.gov (United States)

    Gupta, Radhey S.; Lo, Brian; Son, Jeen

    2018-01-01

    The genus Mycobacterium contains 188 species including several major human pathogens as well as numerous other environmental species. We report here comprehensive phylogenomics and comparative genomic analyses on 150 genomes of Mycobacterium species to understand their interrelationships. Phylogenetic trees were constructed for the 150 species based on 1941 core proteins for the genus Mycobacterium, 136 core proteins for the phylum Actinobacteria and 8 other conserved proteins. Additionally, the overall genome similarity amongst the Mycobacterium species was determined based on average amino acid identity of the conserved protein families. The results from these analyses consistently support the existence of five distinct monophyletic groups within the genus Mycobacterium at the highest level, which are designated as the “Tuberculosis-Simiae,” “Terrae,” “Triviale,” “Fortuitum-Vaccae,” and “Abscessus-Chelonae” clades. Some of these clades have also been observed in earlier phylogenetic studies. Of these clades, the “Abscessus-Chelonae” clade forms the deepest branching lineage and does not form a monophyletic grouping with the “Fortuitum-Vaccae” clade of fast-growing species. In parallel, our comparative analyses of proteins from mycobacterial genomes have identified 172 molecular signatures in the form of conserved signature indels and conserved signature proteins, which are uniquely shared by either all Mycobacterium species or by members of the five identified clades. The identified molecular signatures (or synapomorphies) provide strong independent evidence for the monophyly of the genus Mycobacterium and the five described clades and they provide reliable means for the demarcation of these clades and for their diagnostics. Based on the results of our comprehensive phylogenomic analyses and numerous identified molecular signatures, which consistently and strongly support the division of known mycobacterial species into the five

  1. Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera

    Directory of Open Access Journals (Sweden)

    Radhey S. Gupta

    2018-02-01

    Full Text Available The genus Mycobacterium contains 188 species including several major human pathogens as well as numerous other environmental species. We report here comprehensive phylogenomics and comparative genomic analyses on 150 genomes of Mycobacterium species to understand their interrelationships. Phylogenetic trees were constructed for the 150 species based on 1941 core proteins for the genus Mycobacterium, 136 core proteins for the phylum Actinobacteria and 8 other conserved proteins. Additionally, the overall genome similarity amongst the Mycobacterium species was determined based on average amino acid identity of the conserved protein families. The results from these analyses consistently support the existence of five distinct monophyletic groups within the genus Mycobacterium at the highest level, which are designated as the “Tuberculosis-Simiae,” “Terrae,” “Triviale,” “Fortuitum-Vaccae,” and “Abscessus-Chelonae” clades. Some of these clades have also been observed in earlier phylogenetic studies. Of these clades, the “Abscessus-Chelonae” clade forms the deepest branching lineage and does not form a monophyletic grouping with the “Fortuitum-Vaccae” clade of fast-growing species. In parallel, our comparative analyses of proteins from mycobacterial genomes have identified 172 molecular signatures in the form of conserved signature indels and conserved signature proteins, which are uniquely shared by either all Mycobacterium species or by members of the five identified clades. The identified molecular signatures (or synapomorphies provide strong independent evidence for the monophyly of the genus Mycobacterium and the five described clades and they provide reliable means for the demarcation of these clades and for their diagnostics. Based on the results of our comprehensive phylogenomic analyses and numerous identified molecular signatures, which consistently and strongly support the division of known mycobacterial species

  2. Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil.

    Science.gov (United States)

    Silva, Marcio Roberto; Rocha, Adalgiza da Silva; da Costa, Ronaldo Rodrigues; de Alencar, Andrea Padilha; de Oliveira, Vania Maria; Fonseca Júnior, Antônio Augusto; Sales, Mariana Lázaro; Issa, Marina de Azevedo; Filho, Paulo Martins Soares; Pereira, Omara Tereza Vianello; dos Santos, Eduardo Calazans; Mendes, Rejane Silva; Ferreira, Angela Maria de Jesus; Mota, Pedro Moacyr Pinto Coelho; Suffys, Philip Noel; Guimarães, Mark Drew Crosland

    2013-05-01

    In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB) patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis). Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ) and Stonebrink (SB)-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks). One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6%) of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.

  3. Mixed Cutaneous Infection Caused by Mycobacterium szulgai and Mycobacterium intermedium in a Healthy Adult Female: A Rare Case Report

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    Amresh Kumar Singh

    2015-01-01

    Full Text Available Nontuberculous mycobacteria (NTMs are ubiquitous and are being increasingly reported as human opportunistic infection. Cutaneous infection caused by mixed NTM is extremely rare. We encountered the case of a 46-year-old female, who presented with multiple discharging sinuses over the lower anterior abdominal wall (over a previous appendectomy scar for the past 2 years. Microscopy and culture of the pus discharge were done to isolate and identify the etiological agent. Finally, GenoType Mycobacterium CM/AS assay proved it to be a mixed infection caused by Mycobacterium szulgai and M. intermedium. The patient was advised a combination of rifampicin 600 mg once daily, ethambutol 600 mg once daily, and clarithromycin 500 mg twice daily to be taken along with periodic follow-up based upon clinical response as well as microbiological response. We emphasize that infections by NTM must be considered in the etiology of nonhealing wounds or sinuses, especially at postsurgical sites.

  4. [Usefulness of the variable numbers of tandem repeats (VNTR) analysis for complex infections of Mycobacterium avium and Mycobacterium intracellulare].

    Science.gov (United States)

    Tsunematsu, Noriko; Goto, Mieko; Saiki, Yumiko; Baba, Michiko; Udagawa, Tadashi; Kazumi, Yuko

    2008-09-01

    The bacilli which were isolated from a patient suspected of the mixed infections with Mycobacterium avium and Mycobacterium intracellulare, were analyzed. The genotypes of M. avium in the sedimented fractions of treated sputum and in some colonies isolated from Ogawa medium were compared by the Variable Numbers of Tandem Repeats (VNTR). A woman, aged 57. Mycobacterial species isolated from some colonies by culture in 2004 and 2006 and from the treated sputum in 2006, were determined by DNA sequencing analysis of the 16S rRNA gene. Also, by using VNTR, the genotype of mycobacteria was analyzed. [Results] (1) The colony isolated from Ogawa medium in 2004 was monoclonal M. avium. (2) By VNTR analyses of specimens in 2006, multiple acid-fast bacteria were found in the sputum sediment and in isolated bacteria from Ogawa medium. (3) By analyses of 16S rRNA DNA sequence, M. avium and M. intracellulare were found in the colonies isolated from the sputum sediment and the Ogawa medium in 2006. (4) The same VNTR patterns were obtained in M. avium in 2004 and 2006 when single colony was analyzed. (5) From the showerhead and culvert of the bathroom in the patient's house, M. avium was not detected. By VNTR analyses, it was considered that the mixed infections of M. avium and M. intracellulare had been generated during treatment in this case. Therefore, in the case of suspected complex infection, VNTR analysis would be a useful genotyping method in M. avium complex infection.

  5. Tuberculosis patients co-infected with Mycobacterium bovis and Mycobacterium tuberculosis in an urban area of Brazil

    Directory of Open Access Journals (Sweden)

    Marcio Roberto Silva

    2013-05-01

    Full Text Available In this cross-sectional study, mycobacteria specimens from 189 tuberculosis (TB patients living in an urban area in Brazil were characterised from 2008-2010 using phenotypic and molecular speciation methods (pncA gene and oxyR pseudogene analysis. Of these samples, 174 isolates simultaneously grew on Löwenstein-Jensen (LJ and Stonebrink (SB-containing media and presented phenotypic and molecular profiles of Mycobacterium tuberculosis, whereas 12 had molecular profiles of M. tuberculosis based on the DNA analysis of formalin-fixed paraffin wax-embedded tissue samples (paraffin blocks. One patient produced two sputum isolates, the first of which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, and the second of which only grew on SB media and presented phenotypic profiles of Mycobacterium bovis. One patient provided a bronchial lavage isolate, which simultaneously grew on LJ and SB media and presented phenotypic and molecular profiles of M. tuberculosis, but had molecular profiles of M. bovis from paraffin block DNA analysis, and one sample had molecular profiles of M. tuberculosis and M. bovis identified from two distinct paraffin blocks. Moreover, we found a low prevalence (1.6% of M. bovis among these isolates, which suggests that local health service procedures likely underestimate its real frequency and that it deserves more attention from public health officials.

  6. La Problemática de los fenómenos naturales: potenciales efectos de la erupción del Complejo Volcánico Puyehue-Cordón Caulle (CVPCC sobre la salud humana y el medio ambiente en distintos sectores de la localidad de Villa la Angostura, Neuquén

    Directory of Open Access Journals (Sweden)

    Maria Elena Canafoglia

    2012-12-01

    Full Text Available El trabajo aborda la problemática ambiental de una zona de riesgo volcánico localizada al sur del paralelo 33°, focalizando la atención en el estudio de cenizas de caída reciente o removilizadas por el viento, cinco meses después del inicio de la erupción del Complejo Volcánico Puyehue-Cordón Caulle (CVPCC en junio de 2011. El muestreo se realizó en tres zonas de Villa La Angostura situada a 40 Km al E del foco emisor: (Laguna Piré, Tres Cerros y Escuela CPEM N°17. Considerando los efectos socio-económico-sanitarios de la caída de cenizas en dicha comunidad se ha encarado el estudio de la caracterización físico química de la fracción mas fina del material volcánico (100-5?m. El trabajo, realizado conjuntamente con docentes de la CPEM N°17, se ha efectuado mediante la aplicación de diversas técnicas como difracción por rayos X (DRX, microscopia electrónica (SEM y análisis químico por microsonda (EDS-EDAX. Los resultados de DRX indican que en general la fracción fina es prácticamente amorfa y procede de un proceso de fragmentación de material pumíceo. Sin embargo, eventualmente se observan  algunas líneas de difracción de fases silíceas, particularmente plagioclasa. La morfología del material fino, de composición riolítica (relación SiO2/Al2O3 del orden de 5, se caracteriza por la presencia de bordes agudos y fractura concoide. Presenta contenidos menores en hierro, alcalinos y alcalino-térreos. Se han realizado ensayos químicos adicionales a fin de analizar la alteración del material y su relación con los efectos de corrosión observados en la zona. El conocimiento del material en contacto con la población así como la continuidad en la emisión de particulado fino y la re-movilización del mismo por los vientos, es de vital importancia en lo que se refiere a las acciones de prevención y mitigación de problemas vinculados a la salud y al cuidado del medio ambiente.

  7. Plan de comercialización de zapatos de cuero de avestruz producido por Avestrulandia dirigido al público femenino de Guayaquil

    OpenAIRE

    Cevallos Jácome, Andrés Fernando; Marques Cassagne, José Antonio

    2014-01-01

    La ciudad de Guayaquil es la más poblada del Ecuador, mueve gran parte de la economía del país, su mercado es grande con muchas personas con necesidades por satisfacer, para Avestrulandia complejo ubicado en Chongón la oportunidad de ofrecer sus productos elaborados en cuero de avestruz, en especial del calzado es única, ya que el sector al cual se dirige tiene mucha demanda cada año. El calzado de cuero para dama tiene gran acogida en la ciudad de Guayaquil, debido a que muchas señoras o...

  8. Mycobacterium fortuitum skin infections after subcutaneous injections with Vietnamese traditional medicine: a case report

    NARCIS (Netherlands)

    Lan, Nguyen Phu Huong; Kolader, Marion-Eliëtte; van Dung, Nguyen; Campbell, James I.; Tham, Nguyen Thi; Chau, Nguyen Van Vinh; van Doorn, H. Rogier; Le, Dien Hoa

    2014-01-01

    Iatrogenic skin and soft tissue infections by rapidly growing mycobacteria are described with increasing frequency, especially among immunocompromised patients. Here, we present an immunocompetent patient with extensive Mycobacterium fortuitum skin and soft tissue infections after subcutaneous

  9. AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF MYCOBACTERIUM AVIUM COMPLEX ISOLATES RECOVERED FROM SOUTHERN CALIFORNIA

    Science.gov (United States)

    Fine-scale genotyping methods are necessary in order to identify possible sources of human exposure to opportunistic pathogens belonging to the Mycobacterium avium complex (MAC). In this study, amplified fragment length polymorphism (AFLP) analysis was evaluated for fingerprintin...

  10. First case of Mycobacterium heckeshornense cavitary lung disease in the Latin America and Caribbean region

    NARCIS (Netherlands)

    Coitinho, C.; Greif, G.; Ingen, J. van; Laserra, P.; Robello, C.; Rivas, C.

    2016-01-01

    A case of cavitary pulmonary disease caused by Mycobacterium heckeshornense in Uruguay is described. This is the first case reported in the Latin America and Caribbean region, showing that this species is a worldwide opportunistic human pathogen.

  11. Chronic Mycobacterium marinum Infection Acts as a Tumor Promoter in Japanese Medaka (Oryzias latipes)

    Science.gov (United States)

    An accumulating body of research indicates there is an increased cancer risk associated with chronic infections. The genus Mycobacterium contains a number of species, including M tuberculosis, which mount chronic infections and have been implicated in higher cancer risk. Several ...

  12. Antimicrobial treatment for early, limited Mycobacterium ulcerans infection : a randomised controlled trial

    NARCIS (Netherlands)

    Nienhuis, Willemien A.; Stienstra, Ymkje; Thompson, William A.; Awuah, Peter C.; Abass, K. Mohammed; Tuah, Wilson; Awua-Boateng, Nana Yaa; Ampadu, Edwin O.; Siegmund, Vera; Schouten, Jan P.; Adjei, Ohene; Bretzel, Gisela; van der Werf, Tjip S.

    2010-01-01

    Background Surgical debridement was the standard treatment for Mycobacterium ulcerans infection (Buruli ulcer disease) until WHO issued provisional guidelines in 2004 recommending treatment with antimicrobial drugs (streptomycin and rifampicin) in addition to surgery. These recommendations were

  13. Epidemiology and Ecology of Opportunistic Premise Plumbing Pathogens: Legionella pneumophila, Mycobacterium avium, and Pseudomonas aeruginosa

    Science.gov (United States)

    BACKGROUND: Legionella pneumophila, Mycobacterium avium, and Pseudomonas aeruginosa are opportunistic premise plumbing pathogens (OPPPs) that persist and grow in household plumbing, habitats they share with humans. Infections caused by these OPPPs involve individuals with preexis...

  14. Mycobacterium tuberculosis bacteremia detected by the Isolator lysis-centrifugation blood culture system.

    OpenAIRE

    Kiehn, T E; Gold, J W; Brannon, P; Timberger, R J; Armstrong, D

    1985-01-01

    Mycobacterium tuberculosis was detected by the Isolator lysis-centrifugation blood culture system from the blood of a patient with tuberculosis of the breast. The organism also grew on conventional laboratory media inoculated with pleural fluid from the patient.

  15. Genetic Determinants of Drug Resistance in Mycobacterium tuberculosis and Their Diagnostic Value

    NARCIS (Netherlands)

    Farhat, M.R.; Sultana, R.; Iartchouk, O.; Bozeman, S.; Galagan, J.; Sisk, P.; Stolte, C.; Nebenzahl-Guimaraes, H.; Jacobson, K.; Sloutsky, A.; Kaur, D.; Posey, J.; Kreiswirth, B.N.; Kurepina, N.; Rigouts, L.; Streicher, E.M.; Victor, T.C.; Warren, R.M.; Soolingen, D. van; Murray, M.

    2016-01-01

    RATIONALE: The development of molecular diagnostics that detect both the presence of Mycobacterium tuberculosis in clinical samples and drug resistance-conferring mutations promises to revolutionize patient care and interrupt transmission by ensuring early diagnosis. However, these tools require the

  16. Supplementary Material for: Whole genome sequencing reveals genomic heterogeneity and antibiotic purification in Mycobacterium tuberculosis isolates

    KAUST Repository

    Black, PA; Vos, M. de; Louw, GE; Merwe, RG van der; Dippenaar, A.; Streicher, EM; Abdallah, AM; Sampson, SL; Victor, TC; Dolby, T.; Simpson, JA; Helden, PD van; Warren, RM; Pain, Arnab

    2015-01-01

    Abstract Background Whole genome sequencing has revolutionised the interrogation of mycobacterial genomes. Recent studies have reported conflicting findings on the genomic stability of Mycobacterium tuberculosis during the evolution of drug

  17. Postgenomic approach to identify novel Mycobacterium leprae antigens with potential to improve immunodiagnosis of infection

    NARCIS (Netherlands)

    Geluk, Annemieke; Klein, Michèl R.; Franken, Kees L. M. C.; van Meijgaarden, Krista E.; Wieles, Brigitte; Pereira, Kelly Cristina; Bührer-Sékula, Samira; Klatser, Paul R.; Brennan, Patrick J.; Spencer, John S.; Williams, Diana L.; Pessolani, Maria C. V.; Sampaio, Elizabeth P.; Ottenhoff, Tom H. M.

    2005-01-01

    Early detection of Mycobacterium leprae infection is considered an important component of strategies aiming at reducing transmission of infection, but currently available diagnostic tools often lack sufficient sensitivity and specificity to reach this goal. Recent comparative genomics have revealed

  18. Phylogenetic analysis of vitamin B12-related metabolism in Mycobacterium tuberculosis

    Directory of Open Access Journals (Sweden)

    Douglas B. Young

    2015-03-01

    Full Text Available Comparison of genome sequences from clinical isolates of Mycobacterium tuberculosis with phylogenetically-related pathogens Mycobacterium marinum, Mycobacterium kansasii and Mycobacterium leprae reveals diversity amongst genes associated with vitamin B12-related metabolism. Diversity is generated by gene deletion events, differential acquisition of genes by horizontal transfer, and single nucleotide polymorphisms with predicted impact on protein function and transcriptional regulation. Differences in the B12 synthesis pathway, methionine biosynthesis, fatty acid catabolism, and DNA repair and replication are consistent with adaptations to different environmental niches and pathogenic lifestyles. While there is no evidence of further gene acquisition during expansion of the M. tuberculosis complex, the emergence of other forms of genetic diversity provides insights into continuing host-pathogen co-evolution and has the potential to identify novel targets for disease intervention.

  19. DIGITAL DETECTION SYSTEM DESIGN OF MYCOBACTERIUM TUBERCULOSIS THROUGH EXTRACTION OF SPUTUM IMAGE USING NEURAL NETWORK METHOD

    Directory of Open Access Journals (Sweden)

    Franky Arisgraha

    2012-01-01

    Full Text Available Tuberculosis (TBC is an dangerous disease and many people has been infected. One of many important steps to control TBC effectively and efficiently is by increasing case finding using right method and accurate diagnostic. One of them is to detect Mycobacterium Tuberculosis inside sputum. Conventional detection of Mycobacterium Tuberculosis inside sputum can need a lot of time, so digitally detection method of Mycobacterium Tuberculosis was designed as an effort to get better result of detection. This method was designed by using combination between digital image processing method and Neural Network method. From testing report that was done, Mycobacterium can be detected with successful value reach 77.5% and training error less than 5%.

  20. GENETIC FINGERPRINTING OF MYCOBACTERIUM AVIUM COMPLEX (MAC) ORGANISMS ISOLATED FROM HOSPITAL PATIENTS AND THE ENVIRONMENT

    Science.gov (United States)

    A particularly pathogenic group of mycobacteria belong to the Mycobacterium avium complex (MAC), which includes M. avium and M. intracellulare. MAC organisms cause disease in children, the elderly, and immuno-compromised individuals. A critical step in preventing MAC infections...