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Sample records for aislado del aloe

  1. Estudio del efecto de los esteroles aislados de la planta solanum nudum en la membrana del glóbulo rojo humano

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    Silvia Blair Trujillo

    2004-02-01

    Full Text Available

    En el mundo existen pocas alternativas terapéuticas para malaria. El Grupo Malaria ha aislado esteroides de la planta Solanum nudum, usada para el tratamiento de fiebres por la edicina tradicional colombiana, y ha encontrado que tienen ctividad antimalárica in vitro e in vivo contra P. falciparum, P. vivax y P. berghei. Los compuestos aislados de S. nudum son de tipo esteroidal y se conoce que los esteroles pueden afectar la elación colesterol/fosfolípidos en la membrana del eritrocito 1, igualmente, pueden cambiar su permeabilidad para Na+ y + (2. En los eritrocitos parasitados con Plasmodium la edistribución del colesterol en la membrana y la presencia de roteínas del parásito aumentan la permeabilidad. En la membrana el eritrocito se presentan microdominios: arreglos de ípidos y colesterol a los cuales se anclan las proteínas a través el glicosilfosfatidilinositol. Se ha observado como la extracción el colesterol de los microdominios del eritrocito interfiere en la invasión del glóbulo rojo por Plasmodium (3, sugiriendo que pueden ser aprovechados funcionalmente como blanco erapéutico. El objetivo de este trabajo es determinar el efecto de los esteroles de la planta Solanum nudum en la membrana el eritrocito humano normal e infectado con P. falciparum, mediante el análisis de permeabilidad y de microdominios.

     

     

  2. Determinación de sexo en restos prehistóricos aislados del este uruguayo

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    Figueiro, Gonzalo

    2001-01-01

    Full Text Available El frecuente hallazgo de restos humanos desarticulados y dispersos en sitios de la región Este del territorio uruguayo da lugar a la necesidad de recurrir en muchos casos a métodos alternativos de determinación de sexo, basados en análisis discriminantes. Las muestras de las que se parte en estos análisis son ya de poblaciones actuales, ya de poblaciones prehistóricas distintas a las representadas en el registro arqueológico uruguayo. Al determinar el número mínimo de individuos de un conjunto disperso, la edad y el sexo de los individuos representados cobran gran utilidad, y la precisión de los métodos de determinación de sexo basados en piezas aisladas se vuelve crucial. Este trabajo presenta la evaluación de 24 ecuaciones discriminantes canónicas sobre medidas de 7 huesos, en una muestra arqueológica de 15 individuos de dos sitios con estructuras monticulares ("cerritos de indios" del Este Uruguayo. El sexo de los esqueletos fue determinado anteriormente mediante métodos basados en la pelvis, el cráneo, o ambos. Los métodos evaluados presentaron grados de exactitud general de entre el 30 y el 100 por ciento, sin una dirección particular en los errores de clasificación. Esto pone de relieve la necesidad de revisar las ecuaciones utilizadas, recurriendo ya sea a una reelaboración de los coeficientes utilizados, o a un desplazamiento de los puntos de corte.

  3. Determinación de aislados nativos de pseudomonas desulfurizadoras mediante el estudio del perfil de ácidos grasos

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    Edilberto Silva Gómez

    2010-09-01

    Full Text Available Utilizando CGAR se determinó el contenido de ácidos grasos celulares de doce aislados colombianos, Pseudomonas aeruginosa 17, 18, 19, 20, 21, 22 y 103, Pseudomonas sp 23, 24, 25, 26 y 27 con capacidad desulfurizadora, Pseudomonas aeruginosa ATCC 9027 y 10145, Pseudomonas sp ATCC 39327 y Pseudomonas flúores cens. Se encontraron 53 ácidos grasos diferentes, entre saturados e insaturados de cadena lineal, y principalmente hidroxiácidos y ramificados.

  4. Efectividad del gel de aloe vera en pacientes con piodermitis subagudas

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    Arely Díaz Cifuentes

    2015-06-01

    Full Text Available Introducción: las piodermitis constituyen enfermedades cutáneas de origen bacteriano, catalogadas como enfermedades emergentes ya que se considera tienen carácter epidémico. Estas infecciones en la piel representan un motivo significativo de asistencia a las consultas dermatológicas. Su tratamiento es diverso, con antibióticos y crema de uso tópico, pero también puede ser utilizada el aloe vera en las lesiones, fundamentalmente por el poder emoliente suavizante que ejerce. Objetivo: determinar la efectividad del gel de aloe vera (Sábila en las piodermitis sub agudas, en pacientes que acudieron a la consulta de dermatología del consultorio “El Golfito” en el municipio de Cabimas, Venezuela, en el periodo comprendido entre 1 de enero hasta el 31 de diciembre de 2013. Métodos: se realizó un estudio experimental, exploratorio, correlacional, para determinar la efectividad del gel de aloe vera en las piodermitis sub agudas, en los pacientes, lugar y periodo de tiempo declarados. El universo de estudio estuvo constituido por 150 pacientes que asistieron a la referida consulta, diagnosticados clínicamente con esta entidad; la muestra quedó conformada por 50 pacientes, de ambos sexos y menores de 70 años. Se indicó tratamiento con antibiótico oral, amoxicillina en dosis de 50mg/kg al día dividida en tres dosis (cada 8 horas en niños hasta 15 años, a partir de esta edad la dosis fue de 500 mg cada 8 horas por 7 días y el uso tópico del gel del aloe en las lesiones tres veces al día hasta lograr la curación. Resultados: el grupo de edad más afectado estuvo en los menores de diecinueve años (42%; en las condiciones socioeconómicas de las familias e higiénico sanitaria de las viviendas predominaron las evaluadas de regular (74% - 70%. Las lesiones elementales más frecuentes al inicio fueron las exulceraciones (86%, mientras que a los 7 y 15 días predominan las máculas (82% - 96%. Se logra la curación en el 82% de los

  5. Actividad antioxidante de compuestos aislados del residuo no-volátil del aceite esencial de naranja.

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    Vargas-Arispuro, L.

    1998-04-01

    Full Text Available There has been a growing interest in the food industry and in preventive medicine to search for «natural antioxidants». Therefore the objetive of this study was to evaluate the antioxidant activity of the non-volatile residue of essential orange oil in the oxidation of unrefined and freshly extracted olive oil. Essential orange oil was distilled to obtain a non-volatile residue and fracctionated on a Silica Gel 60 column. The antioxidant activity was determined by measuring peroxide value obtained after heating olive oil at 70 °C for 48 h. Six compounds were isolated and purified, four of these had antioxidant activity, α-tocopherol and three methoxylated flavones were identified using spectroscopic methods. This study confirmed that non-volatile fractions of essential orange oil contain natural antioxidants diferent from α-tocopherol.

    Existe un creciente interés en la industria alimentaria y en la medicina preventiva por la búsqueda de «antioxidantes naturales», por lo que nos planteamos evaluar la actividad antioxidante del residuo no volátil del aceite esencial de naranja contra la oxidación del aceite de oliva sin retinar y de reciente extracción. Mediante destilación del aceite esencial de naranja se obtuvo el residuo no-volátil, el cual se fraccionó en columna de silica gel 60. La actividad antioxidante fue medida por el valor de peróxidos producidos por el aceite de oliva al provocar la oxidación por calentamiento a 70 °C durante 48 h. Se aislaron seis compuestos mediante HPLC preparativo de los cuales cuatro tuvieron actividad antioxidante. Usando métodos espectroscópicos se identificaron α-tocoferol y tres flavonas metoxiladas. Con este estudio se confirmó que la fracción no volátil del aceite esencial de naranja contiene antioxidantes naturales diferentes al α-tocoferol.

  6. Determinación del efecto del gel de Aloe vera, sobre la glicemia en caninos sanos

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    María Mercedes Tamayo

    2011-02-01

    Full Text Available El papel desempeñado por la glucosa en los organismos es  fundamental, ya que es  la forma más importante por la cual los carbohidratos son absorbidos desde el tracto intestinal, con diversos factores que  influyen en los niveles de glucosa del  organismo.  La  causa más  frecuente  de hiperglucemia  en  los  perros  es  el  daño pancreático, hiperadrenocorticismo  e hipersomatotropismo. Así, al evaluar el efecto del gel de Aloe vera sobre  la glicemia en caninos,tras su administración oral, se utilizaron 18 ejemplares sanos que  fueron distribuidos al azar en seis grupos, en los que se administró diferentes sustancias y se procedió a realizar el exámen de glicemia cada 15 minutos  (min, obteniéndose que la administración de 50 mL de gel de Aloe vera, tanto a caninos normoglicémicos, como a los que se les indujo hiperglucemia, no produjo cambios significativos en los niveles de glucosa.Los  resultados obtenidos  bajo  las  condiciones planteadas en este estudio experimental no validaron el uso empírico tradicional del gel de Aloe vera.

  7. Microagregación genética y geográfica de aislados del virus linfotrópico humano tipo I (HTLV-I en zonas endémicas del suroccidente de Colombia.

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    Felipe García

    2009-10-01

    Full Text Available Con el objetivo de definir el polimorfismo de patrones de restricción (RFL para la endonucleasa de restricción DdeI, de la región del HTLV-I de los 5181 a los 6624 nucleótidos, se amplificó un segmento de 1033 bp que incluía la porción terminal 5' del gen Pol y el dominio de superficie del gen Env (gp46 del ADN proviral de PBMC obtenido de 29 personas seropositivas para el HTLV-I provenientes de varias zonas el suroccidente de Colombia. El análisis de restricción efectuado con la endonucleasa DdeI, reveló la existencia de tres patrones de RFLPs diferentes. El patrón I (900 y 125 pb, se observó en 34.5% (10/29 de los aislados. El patrón IIa (700, 205 y 125 pb se determinó en 51.7% (15/29. Finalmente, el patrón IIb (550, 350 y 125 pb representó 13.8% (4/29 aislados. El microagregado IIb se observó con predominio en aislados de HTLV-I del municipio de Tumaco. Los patrones I y IIa se distribuyeron con mayor frecuencia en el interior del suroccidente. Los resultados obtenidos muestran la existencia de un mecanismo de microevolución divergente de la región pol-env en los virus de las áreas analizadas.

  8. El uso terapéutico del Aloe Vera en las Úlceras Por Presión (UPP

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    Francisco José Hernández Martínez

    2010-01-01

    Full Text Available El Aloe Vera es un excelente limpiador y antiséptico natural que penetra fácilmente en la piel y tejidos, con gran actividad bactericida, fungicida, antiinflamatorio, antiprurítico, dilata los capilares sanguíneos, descompone y destruye los tejidos muertos y favorece el crecimiento celular. El objetivo de este trabajo fue averiguar si el uso de Aloe Vera en UPP es eficaz como tratamiento. Estudio cualitativo de 59 pacientes con UPP ingresados en un Hospital Geriátrico, realizando un protocolo de curas con una cataplasma de Aloe Vera triturado. La edad media fue de 76.2% años. Distribución de la muestra: 13 pacientes con UPP Grado I, 21 Grado II ,15 Grado III y 10 Grado IV.El 99% de grado I evolucionaron fenomenalmente al tratamiento con Aloe, todas desaparecieron entre 3 y 7 días. El 1% presentó una alergia al Aloe triturado.UPP grado II, aparece mejoría a las 24 horas y todas curaron entre 12-14 días con una ligera variación del color de la piel. Grado III y IV evolucionaron de forma diferente a las anteriores. El 5% grado III terminaron curándose, 95% restante sufrieron modificaciones del tejido afectado; disminuyendo el exudado y favoreciendo la coloración. A partir de 4 ó 5 días la musculatura y tejidos implicados en la UPP, variaban su coloración y el lecho se secaba. En el 98% de Grado IV aparecía enrojecimiento y quemaduras después de aplicar Aloe. El Aloe Vera puede ayudar a curar UPP Grado I y II. Dudosa evidencia científica para uso, en UPP Grado III y IV. Un porcentaje pequeño ha presentado reacciones adversas de tipo alérgico.

  9. Determinación del efecto del gel de Aloe vera, sobre la glicemia en caninos sanos

    OpenAIRE

    María Mercedes Tamayo; Anastasia Cruz-Carrillo

    2011-01-01

    El papel desempeñado por la glucosa en los organismos es  fundamental, ya que es  la forma más importante por la cual los carbohidratos son absorbidos desde el tracto intestinal, con diversos factores que  influyen en los niveles de glucosa del  organismo.  La  causa más  frecuente  de hiperglucemia  en  los  perros  es  el  daño pancreático, hiperadrenocorticismo  e hipersomatotropismo. Así, al evaluar el efecto del gel de Aloe vera sobre  la glicemia en caninos,tras su administración oral, ...

  10. Dinámica poblacional del rotífero Brachionus ibericus aislado de estanques para camarón, alimentado con diferentes dietas

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    José Cristóbal Román-Reyes

    2014-11-01

    Full Text Available La producción de microalgas vivas para cultivar rotíferos constituye uno de los mayores costos de operación en la larvicultura de peces, por lo que se están desarrollando sustitutos comerciales de microalgas para la alimentación y producción de rotíferos. Se desconoce el efecto que tienen las formulaciones comerciales sobre la dinámica poblacional de rotíferos nativos del noroeste de México y en este estudio se evaluó el efecto de cuatro dietas comerciales (Espirulina, RotiMac®, Nanno 3600®, RotiGrow-Plus® y la microalga viva Nannochloropsis sp., sobre el crecimiento poblacional y fecundidad del rotífero Brachionus ibericus (GenBank KJ949043, aislado de una granja de camarón blanco (Litopenaeus vannamei. Se realizaron cinco réplicas por tratamiento alimenticio utilizando recipientes con volumen de 12 L de agua, con temperatura de 29 ± 1°C y salinidad de 35 ± 1, respectivamente. Los resultados mostraron que durante los periodos experimentales, las dietas probadas resultaron adecuadas para alimentar a B. ibericus, con tasas de crecimiento, tiempos de duplicación, densidades máximas y fecundidades que fluctuaron entre 0.20 a 0.27 rotíferos día-1, 2.60 a 3.42 días, 215 a 344 rotíferos mL-1 y de 0.16 a 0.39 huevos hembra-1, respectivamente. Las diferencias entre tratamientos fueron significativas (P 0.05 a las obtenidas con Nanno 3600®.

  11. ESTUDIO MORFOLÓGICO DEL CULTIVO A LARGO PLAZO DE FOLÍCULOS AISLADOS Y CERRADOS DE TIROIDES DE CERDO

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    M HERRERA

    2008-01-01

    Full Text Available La unidad morfológica y funcional de la glándula tiroides es el folículo, estructura ovoide cerrada, constituida por una capa de células cúbicas (tirocitos que encierran un lumen lleno del coloide secretado por ellas. En cultivo, tanto la estructura como la función del folículo se pierden rápidamente en las primeras 24 horas. Sin embargo, si se cultivan folículos cerrados de tiroides de rata conservan la arquitectura folicular, la morfología del tirocito y la función hasta la síntesis de hormonas tiroides de manera similar a la glándula in vivo. En este trabajo describimos el aislamiento y cultivo de folículos porcinos cerrados y su análisis morfológico. Los folículos se aíslan por digestión enzimática y disociación mecánica del parénquima tiroideo, luego se cultivan sobre agarosa con y sin hormona tirotrópica o tirotropina (1 mU/ml, TSH. El tejido de tiroides porcino obtenido tiene las mismas características de una glándula hipotiroidea in vivo, un epitelio casi plano, retículo endoplásmico rugoso (RER exiguo, complejo de Golgi (CG, y microvellosidades escasas y cortas. Los folículos cultivados sin TSH conservan la forma ovoide y el coloide en su interior, y la misma ultra-estructura del tejido in vivo, RER y CG muy escasos, pero con el tiempo de cultivo aumenta la longitud de la microvellosidades y el espesor del epitelio. En presencia de TSH el epitelio se hipertrofia desde el primer día y las cavidades foliculares se reducen considerablemente. Se demuestra que folículos cerrados de tiroides durante ocho días (d de cultivo conservan su morfología con y sin TSH. Además, estos responden al estímulo de TSH disminuyendo su cavidad folicular y aumentando el espesor del epitelio folicular.

  12. Estudio del efecto de los fructooligosacáridos en la producción de bacteriocinas por aislados nativos de Lactobacillus spp

    OpenAIRE

    2012-01-01

    En el presente estudio se evaluó el efecto de los fructooligosacáridos (FOS) sobre e crecimiento y producción de bacteriocinas por cepas nativas de Lactobacillus aisladas de ensilaje de maíz y melaza. Se obtuvo un total de 28 aislados nativos que presentaron características bioquímicas comparables a especies comerciales de Lactobacillus. La presencia de FOS en el medio de cultivo mostró un efecto prebiótico frente a todos los aislados y cepas comerciales favoreciendo el aumento de la població...

  13. Caracterización molecular y agronómica de aislados de Trichoderma spp nativos del noreste de México

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    José Luis Hernández Mendoza

    2012-06-01

    Full Text Available Normal 0 21 false false false ES-CO X-NONE X-NONE MicrosoftInternetExplorer4 Título en ingles: Molecular and agronomic characterization of Trichoderma spp natives of northeastern Mexico  Resumen Trichoderma sp es un hongo frecuentemente usado en actividades agrícolas, pues actúa como antagonista de diversas especies de hongos fitopatógenos. En este estudio se realizó el aislamiento de cuatro cepas de Trichoderma sp nativas del noreste de México, las cuales fueron identificadas a nivel molecular mediante la secuenciación del ITS 1. Además se evaluó su capacidad antagonista en contra los hongos fitopatógenos Macrophomina phaseolina y Fusarium oxysporum, que afectan severamente cultivos de sorgo, maíz y fríjol en el noreste de México. La identificación se realizó de acuerdo al grado de concordancia con secuencias reportadas y corresponden a las especies T. hammatum (HK701;  T. koningiopsis (HK702; T. asperellum (HK703 y Trichoderma sp (HK704. Por otra parte, las pruebas de antagonismo muestran que los aislados HK701, HK703 y HK704 inhiben por competencia el crecimiento de M. phaseolina y F. oxysporum, mientras que HK702 tiene la capacidad para hiperparasitar dichos fitopatógenos. Finalmente, se evaluó la promoción de crecimiento de T. asperellum HK703, en maíz (Pionner 30P49®, usando para ello concentraciones de tratamiento de 1x10e2 hasta 1x10e6 esp/mL. En estos ensayos se midió la producción de biomasa. Los resultados muestran que en concentraciones intermedias se tiene el mayor incremento en altura de plantas y mayor producción de peso seco en follaje y raíz. Entre los parámetros antes mencionados existen diferencias significativas. Palabras clave: control biológico, antagonismo, biofertilizantes, Zea maiz, Sorghum bicolor. Abstract Trichoderma sp is a fungus often used in agricultural activities, because it acts as an antagonist of several species of plant pathogenic fungi. In this study four strains of Trichoderma

  14. Efecto de la temperatura sobre películas de un aislado proteico del suero bovino (WPI adsorbidas sobre la interfase aceite-agua

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    Rodríguez Patino, Juan M.

    2002-09-01

    Full Text Available Heat-induced interfacial aggregation of a whey protein isolate (WPI with a high content of β-lactoglobulin, previously adsorbed at the oil-water interface, was studied by interfacial dynamic characteristics (interfacial tension and surface dilational properties performed in a automatic drop tensiometer coupled with microscopic observation and image analysis of the drop after heat-treatment. The temperature, ranging between 20 and 80 ºC, and protein concentration in aqueous bulk phase, ranging between 1.10-1 and 1.10-5% wt/wt, were studied as variables. The pH, and ionic strength were maintained constant at 5 and 0.05 M, respectively. During the heat-treatment, WPI films behave typically as viscoelastic with non-zero phase angle, but with increasing elastic characteristics as the heat-treatment progresses. During isothermal treatment the surface dilational modulus, E, increases and the interfacial tension, σ, and phase angle, φ, decrease with time to a plateau value. The time dependence of E can be quantified by a first-order equation according to two kinetic mechanisms. The rate of thermal changes in WPI adsorbed films increases with protein concentration in solution. Heat-treatment produces irreversible changes in WPI adsorbed films because the interfacial characteristics do not return to original values after cooling back to the initial temperature. Significant changes in interfacial characteristics and drop image associated with interfacial WPI gelation were observed at protein concentration as low as 1.10-5% wt/wt, even for heat-treatment at 40 ºC.En este trabajo se ha estudiado la agregación interfacial inducida por el calor en un aislado proteico del suero bovino (WPI, previamente adsorbido sobre la interfase aceite-agua. Se ha seguido la evolución, durante el tratamiento térmico, de las características dinámicas interfaciales (tensión interfacial y propiedades dilatacionales superficiales, determinadas en un tensi

  15. Modificaciones del patrón fibrilatorio ventricular por el estiramiento regional del ventrículo izquierdo: estudio en corazón aislado de conejo.

    OpenAIRE

    Trapero Gimeno, Isabel

    2006-01-01

    RESUMEN Introducción: Se parte de la hipótesis de que las modificaciones locales de las propiedades electrofisiológicas, si se circunscriben a zonas de extensión limitada, deben causar escasos cambios en el proceso global de activación durante la fibrilación ventricular (FV). Para comprobarlo, se produce un estiramiento local del miocardio, en la pared libre del ventrículo izquierdo, y se analizan diversos parámetros electrofisiológicos, tanto en situación de ritmo sinusal como durante la ...

  16. GEL DE ALOE-VERA COMO POTENCIAL INHIBIDOR DE LA CORROSIÓN DEL ACERO DE REFUERZO ESTRUCTURAL

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    Héctor Herrera-Hernández

    2015-01-01

    Full Text Available En el presente trabajo se estudiaelectroquímicamente un GELextracto de las hojas de ALOE VERAcomo un posible inhibidor de la corrosión del acero de refuerzo estructuraldel concreto.Los estudiosde corrosiónse llevaron a cabo en 1M de HCl y utilizando la técnica de espectroscopia de impedancia electroquímica (EISse evaluó la resistencia a la corrosión en presencia y ausencia del GEL.Los diagramas de impedancia mostraronque la adición en diferentes proporcionesdel GELinhibe notablemente el proceso de la corrosión del acero inmerso en la solución ácida. También se determinó que las moléculas del GEL obedecena un mecanismo de fisisorciónsobre la superficie del metal de acuerdo con el modelo de isoterma de Langmuircon unG°adsde alrededor de 14.17 kJ/mol. El GEL se clasificócomo un inhibidor orgánico del tipo mixto.

  17. DEGRADACIÓN DEL ALDRÍN POR Bacillus licheniformis, AISLADO DEL AGUA Y SEDIMENTO DE LA CIENAGA GRANDE DE SANTA MARTA

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    Sánchez Díaz Granados José Gregorio

    2012-04-01

    Full Text Available Con el objeto de apoyar la utilización de los microorganismos como alternativa para la degradación de contaminantes orgánicos persistentes, se aisló la bacteria Bacillus licheniformis, a partir de muestras de sedimento y agua del complejo lagunar de la Ciénaga Grande de santa Marta (CGSM, Caribe colombiano; capaz de tolerar y degradar en condiciones aerobias el plaguicida organoclorado aldrín. Se realizó un bioensayo en el que se expuso al B. licheniformis a una concentración de 60ng/L de aldrín, durante un período de 30 días se evaluó la capacidad degradadora de la bacteria sobre el organoclorado. La identificación y aislamiento de B. licheniformis, se realizó a través de caracterización macroscópica y microscópica y pruebas bioquímicas (sistema BBL Crystal y la determinación de las concentraciones de aldrín con la técnica de cromatografía de gases. Los resultaron mostraron que B. licheniformis posee capacidad degradadora de un 24% del aldrín y que los factores como la exposición a la luz solar y la volatilización influyen considerablemente en la degradación del organoclorado con una reducción adicional de 31%.

  18. CARACTERIZACIÓN FENOTÍPICA DE AISLADOS DE RIZOBIOS PROCEDENTES DE LA LEGUMINOSA FORRAJERA Canavalia ensiformis

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    Ionel Hernández Forte

    2012-01-01

    Full Text Available El objetivo del presente trabajo fue caracterizar fenotípicamente aislados de rizobios procedentes de la leguminosa forrajera Canavalia ensiformis. Se caracterizaron doce aislados, cinco posibles miembros del género Rhizobium/ Sinorhizobium, siete posibles integrantes del género Bradyrhizobium y dos cepas comerciales, BR 2001 y BR 2003a. Para la caracterización fenotípica de los aislados bacterianos se estudió su capacidad de utilizar seis fuentes de carbono, la tolerancia a condiciones de acidez y a diferentes niveles de cloruro de sodio (NaCl, la resistencia a seis antibióticos así como su crecimiento en tres temperaturas de incubación. Se determinó además la capacidad de estos aislados de producir polihidroxibutiratos (PHB. Todos los aislados estudiados utilizaron la lactosa, glucosa y manitol como fuentes de carbono. Uno de los aislados de rápido crecimiento resultó el más tolerante a pH 4.5. Los aislados posibles miembros del género Rhizobium/ Sinorhizobium toleraron una mayor concentración de NaCl que los posibles integrantes del género Bradyrhizobium. Tres de los aislados de rápido crecimiento fueron resistentes a los seis antibióticos utilizados. Cuando los aislados fueron incubados a 370C todos, con excepción del el aislado CP3 y la cepa comercial BR 2001, presentaron un crecimiento similar al control. El aislado CP10, de lento crecimiento, tuvo la capacidad de producir las mayores concentraciones de PHB (0.023 g.L-1.

  19. Determinación del potencial promotor del crecimiento vegetal de Azotobacter spp. aislados de la rizósfera de malezas en cultivos de maíz (Zea mays L.

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    Luis H. León

    2015-01-01

    Full Text Available Los fertilizantes químicos representan entre 20% y 30% de los costos de producción de un cultivo, utilizados correctamente incrementan la productividad y rentabilidad; sin embargo, cada año aumenta la cantidad de fertilizantes por aplicar, debido a la deficiencia de adsorción en el suelo y absorción por la planta. Siendo el maíz el tercer cultivo de importancia en Perú, con un impacto significativo en la actividad económica y social, en el 2014, solo el 40% del maíz ofertado correspondió a la producción nacional. En busca de alternativas para disminuir el uso de fertilizantes químicos se realizan investigaciones con denominadas rizobacterias promotoras del crecimiento vegetal (PGPR, por sus siglas en ingles. Se identificaron 37 malezas en cultivos de maíz procedentes de campos agrícolas de los distritos de Monsefú y Reque, Región Lambayeque, siendo dicotiledóneas predominantes con 68 % con respecto a monocotiledóneas con 32%. Las bacterias se aislaron de la rizósfera de malezas, obteniendo 305 cultivos puros de bacterias, de los cuales 133 cultivos puros (43,7% se identificaron como Azotobacter spp., investigándose su reacción bioquímica en reducción de nitratos, utilización de sacarosa, glucosa, maltosa, manitol, ramnosa, glicerol y sorbitol, identificándose A. vinelandii (58%, A. paspali (13%, A. armeniacus (8%, A. nigricans (8% y en 13 cultivos no se identificó la especie. Con los cultivos Azotobacter spp. se cuantificó hasta 36,03 ppm de nitrógeno fijado como amonio; 60,75 ppm de ácido indol acético y 6,06 ppm de fósforo solubilizado, se determinó actividad antagónica contra Fusarium verticillioides, proteolítica y quitinolítica y. El 20% de Azotobacter spp. no afectó la emergencia de maíz amarillo duro hibrido simple AGRI- 144, el 33 % la afectó positivamente y el 47% la afectó negativamente. A su vez, ningún Azotobacter spp. afectó la sobrevivencia. Demostrándose el potencial promotor del

  20. Aloe vera

    Energy Technology Data Exchange (ETDEWEB)

    Klein, A.D.; Penneys, N.S.

    1988-04-01

    We review the scientific literature regarding the aloe vera plant and its products. Aloe vera is known to contain several pharmacologically active ingredients, including a carboxypeptidase that inactivates bradykinin in vitro, salicylates, and a substance(s) that inhibits thromboxane formation in vivo. Scientific studies exist that support an antibacterial and antifungal effect for substance(s) in aloe vera. Studies and case reports provide support for the use of aloe vera in the treatment of radiation ulcers and stasis ulcers in man and burn and frostbite injuries in animals. The evidence for a potential beneficial effect associated with the use of aloe vera is sufficient to warrant the design and implementation of well-controlled clinical trials. 27 references.

  1. Histophilus somni (Haemophilus somnus) aislado en casos de problemas del aparato reproductor de ganado lechero. Primer informe en México

    OpenAIRE

    Francisco Aguilar Romero; Francisco José Trigo Tavera; Enrique Herrera López; Jorge Ávila García; Francisco Suárez Güemes

    2005-01-01

    Histophilus somni (Haemophilus somnus) es reconocido como un patógeno importante de los bovinos, asociado a desórdenes reproductivos como vaginitis, endometritis, infertilidad y aborto. El objetivo del presente trabajo fue determinar si H. somni se encuentra presente en vacas lecheras con enfermedades del aparato reproductor. Se realizó un muestreo de conveniencia en seis establos de la zona central de México, en donde se colectaron muestras de exudado vaginal de 67 vacas enfermas del tracto ...

  2. Inestabilidad cariológica durante la formación de células madres del polen en Aloe vera (Aloaceae)

    OpenAIRE

    Imery Buiza, José

    2007-01-01

    Con el objetivo de esclarecer la posible existencia de anomalías citogenéticas que aminoren la fertilidad del polen de Aloe vera, se analizó la etapa de proliferación celular que lleva a la formación de células madres del polen (CMPs). Se recolectaron botones florales (BF) en 25 plantas de una población ubicada a 10°34'15'' N, 64°12'08'' W, los cuales fueron fijados en Carnoy I por 24 h y almacenados en etanol (70 % v/v). Las observaciones se realizaron en preparaciones temporales obtenidas p...

  3. Caracterización molecular y agronómica de aislados de Trichoderma spp nativos del noreste de México

    OpenAIRE

    José Luis Hernández Mendoza; María Isabel Sánchez Pérez; Jesús Gerardo García Olivares; Netzahualcoyotl Mayek Pérez; Juan Manuel González Prieto; Jesús Di Carlo Quiroz Velásquez

    2011-01-01

    Título en ingles: Molecular and agronomic characterization of Trichoderma spp natives of northeastern Mexico  Resumen Trichoderma sp es un hongo frecuentemente usado en actividades agrícolas, pues actúa como antagonista de diversas especies de hongos fitopatógenos. En este estudio se realizó el aislamiento de cuatro cepas de Trichoderma sp nativas del noreste de México, las cuales fueron identificadas a nivel molecular mediante la secuenciación del ITS 1. Además se evaluó su capacidad antag...

  4. Detección de los genes que codifican superantígenos en staphylococcus aureus aislados de madres comunitarias del instituto colombiano de bienestar familiar de Pereira

    OpenAIRE

    Flórez Restrepo, Gladys Fernanda

    2015-01-01

    La intoxicación alimentaria es un importante problema de salud pública a nivel mundial, en la intoxicación alimentaria muchos manipuladores de alimentos pueden ser portadores sanos de cepas de S. aureus en sus fosas nasales o en sus manos, estos son considerados como una de las principales fuentes de contaminación en alimentos, a través del contacto manual o mediante las secreciones respiratorias. El propósito del presente estudio fue detectar los genes que codifican los superantígenos en S. ...

  5. ACTIVIDAD FIBROLÍTICA DE HONGOS RUMINALES AISLADOS DE ECOSISTEMAS TROPICALES

    Directory of Open Access Journals (Sweden)

    L Gualdrón

    2007-01-01

    Full Text Available Se evaluó la capacidad de degradación de sustratos altamente Àbrosos por 18 aislados funga-les ruminales en fermentaciones de 120 horas utilizando como única fuente de carbono pasto colosuana (Bothriochloa pertusa. Dentro del ensayo se incluyeron aislados pertenecientes a los géneros Neocallimastix, Orpinomyces y Piromyces. De acuerdo con los parámetros del modelo de Gompertz, la fase de adaptación de los aislados fue de 23,8 horas (h ± 4,20, con una producción de gas promedio de 195 ml/g sustrato ± 7,52, con un tiempo en alcanzar la mitad de la producción de gas entre las 35 y 55 h. La mayor tasa máxima de producción de gas (TMPG fue de 5,7 ml/h, perteneciente a un aislado del género Orpinomyces. Se observó una degradación de la materia seca (MS de 58% ± 2, y de la Àbra en detergente neutro (FDN de 46% ± 2 en promedio para todos los aislados fungales. La producción total de ácidos grasos volátiles (AGV fue de 22,04 ± 1,5 mM, con una proporción molar de ácido acético de 0,93% ± 0,01 para el promedio de los 18 aislados. Los valores máximos de las actividades enzimática carboximetilcelulasa (CMCasa, celulasa microcristalina y xilanasa fueron 9,56, 1,39 y 303,12 UI respectivamente, conÀrmando la preferencia de los hongos ruminales hacia los sustratos hemicelulósicos. Se realizó un análisis de factores principales, y los aislados se agruparon por medio de un análisis de conglomerados. Fueron selecciona-dos cinco aislados fungales los cuales presentaron la mayor diversidad en los parámetros evaluados, principalmente en degradación de MS, FDN y las actividades enzimáticas. Se seleccionó un aislado del género Neocallimastix, dos del género Orpinomyces, y dos del género Piromyces.

  6. Remoción de Cromo Hexavalente por el Hongo Paecilomyces sp. Aislado del Medio Ambiente Hexavalent Chromium Removal by a Paecilomyces sp Fungal Strain Isolated from Environment

    Directory of Open Access Journals (Sweden)

    Juan F Cárdenas-González

    2011-01-01

    Full Text Available Se aisló un hongo resistente y capaz de remover cromo hexavalente a partir del medio ambiente de una zona cercana a la Facultad de Ciencias Químicas, Universidad de San Luis Potosí en México. La cepa fue identificada como Paecilomyces sp, en base a sus características macro y microscópicas. La biomasa fúngica remueve eficientemente Cromo (VI en solución y puede utilizarse para descontaminar nichos acuáticos contaminados, ya que 1 g de biomasa fúngica remueve 100 y 1000 mg/100 mL del metal a una y tres horas de incubación, y elimina totalmente 297 mg Cr(VI/g de tierra contaminada.A fungal strain resistant to Cr (VI and capable of removing the oxyanion from the médium was isolated from the environment near the Chemical Science Faculty, University San Luis Potosí in México. The strain was identified as Paecilomyces sp, by macro and microscopic characteristics. It was concluded that this fungal biomass can be used for the removal of Cr (VI in aqueous solutions, since 1 g of fungal biomass removes 100 y 1000 mg/100 mL of this metal after one and three hours of incubation, and removes 297 mg Cr (VI from contaminated soil.

  7. Validación de un método analítico para el estudio de estabilidad del ungüento de aloe

    OpenAIRE

    García Peña, Caridad M.; Romero Díaz, Jacqueline A.; Muñoz Cernuda, Adriana; Martínez Espinosa, Vivian

    2008-01-01

    En el presente trabajo se desarrolló y validó un método espectrofotométrico para el control de la calidad del ungüento de aloe al 25%. En la validación se evaluaron los parámetros de especificidad, linealidad, exactitud y precisión expresada en sus dos formas: repetibilidad y precisión intermedia. El método desarrollado resultó selectivo, lineal, preciso, robusto y exacto, en el rango de concentraciones estudiadas.

  8. Aloe vera on Lanzarote

    OpenAIRE

    Walker, Colin

    2016-01-01

    Aloe vera is the basis of a multibillion dollar cosmetics and toiletries industry worldwide. It is grown commercially in many parts of the world and here its cultivation is described on an aloe farm in the north of Lanzarote, the Canary Islands. The products from such monoculture are sold throughout the island in Aloe vera shops run principally by the company aloe Lanzarote.

  9. Comparación antigénica y de la respuesta inmune en ratones desafiados con virus CVS y aislados «calle» y «fijo»presumiblemente atípicos del virus rábico Antigenic comparison and immune response in challenges mice with virus CVS and "street" and "fixed" isolates presumably atipics of rabies virus

    Directory of Open Access Journals (Sweden)

    P. MARTINEZ

    1999-01-01

    Full Text Available El virus de la rabia presenta diferencias antigénicas que han sido detectadas en diferentes regiones y países, lo que podría acarrear un serio problema en relación a las vacunas antirrábicas empleadas en terreno que no se corresponden con las cepas actuantes pudiendo provocar fracaso del tratamiento vacunal preventivo o post-exposición. Ante la sospecha de la existencia de virus rabia presuntamente atípicos en nuestro país, nos planteamos como objetivo estudiar el comportamiento antigénico de aislados vírales "calle" y "fijo" en relación a la cepa estándar, Challenge Virus Standar, frente a las vacunas antirrábicas veterinaria y de referencia nacional, y el suero antirrábico hiperinmune de referencia nacional. Se estudiaron 6 aislados de virus rábico presuntamente atípicos (5 de murciélagos y 1 de gato obtenidos en 1985 en el Instituto de Salud Pública y mantenidos a -70ºC en cerebro de ratón lactante. Para ello, primeramente, con la finalidad de lograr la fijación de los aislados a los animales de laboratorio, se procedió a realizar pasajes sucesivos en CRL, confirmando en cada pasaje la causa de muerte a través de la prueba de inmunofluorescencia directa. Para determinar la protección conferida en ratones, por las vacunas tradicionales frente a los aislados presuntamente atípicos, se compararon las respuestas de ratones vacunados y desafiados con los 6 aislados en su forma "calle" y "fija", con las respuestas de los ratones vacunados y desafiados con la cepa de referencia del virus estándar de desafío CVS. Para conocer la capacidad de neutralización del suero de referencia nacional frente a los aislados vírales "fijo", se compararon los resultados de neutralización en ratones, de cada uno de los aislados enfrentados al suero de referencia nacional, con la neutralización de la cepa CVS enfrentada al mismo suero. La fijación se logró en el pasaje Nº 16, para los aislados 85/423, 85/433, 85/684, 85/882, 85

  10. Efectos del extracto de croton schiedeanus sobre la producción de oxido nítrico en la preparación de anillos aislados de aorta / Effects of croton schiedeanus extract on nitric oxide production in the preparation of isolated aortic rings

    OpenAIRE

    Chaves Torres, Ninfa Marlen

    2010-01-01

    En este trabajo se determinó la importancia de la ruta metabólica del óxido nítrico y la guanilato ciclasa en el efecto vasodilatador inducido por el extracto de Croton Schiedeanus Schlecht utilizando la preparación de anillos aislados de aorta de ratas Wistar. El extracto de C. Shiedeanus relaja anillos precontraídos con fenilefrina, agonista del receptor adrenérgico alfa, en función de la dosis, con una CE50 de 1,3x10-5 g/mL (9,1x10-6 – 2x10-5). Este efecto es superior al ejercido sobre ani...

  11. Microagregación genética y geográfica de aislados del virus linfotrópico humano tipo I (HTLV-I) en zonas endémicas del suroccidente de Colombia.

    OpenAIRE

    Felipe García; Mónica Chávez; Martha Cecilia Domínguez; Abraham Blank

    2009-01-01

    Con el objetivo de definir el polimorfismo de patrones de restricción (RFL) para la endonucleasa de restricción DdeI, de la región del HTLV-I de los 5181 a los 6624 nucleótidos, se amplificó un segmento de 1033 bp que incluía la porción terminal 5' del gen Pol y el dominio de superficie del gen Env (gp46) del ADN proviral de PBMC obtenido de 29 personas seropositivas para el HTLV-I provenientes de varias zonas el suroccidente de Colombia. El análisis de restricción efectuado con la endonuclea...

  12. Vigilancia del dengue basada en el laboratorio: diferencias en el número de casos y virus aislados según la recolección del suero y la prueba serológica.

    Directory of Open Access Journals (Sweden)

    Raquel E. Ocazionez

    2009-11-01

    Full Text Available Introducción: El control del dengue depende de los datos de la vigilancia basada en el laboratorio. El objetivo de este estudio fue establecer las diferencias en número de casos (IgM positiva y serotipos del virus según la estrategia de vigilancia. Materiales y métodos: Se compararon los resultados de dos estrategias de vigilancia. Primera (SIVIGILA local: de 337 casos con sospecha clínica de dengue se colectó un único suero (agudo o convaleciente que se almacenó a 4oC. Se utilizó el estuche UMELISA DENGUE (IPK, Cuba para detectar anticuerpos IgM y se hicieron intentos de aislamiento del virus en células C6/36. Segunda: de 318 casos sospechosos se colectaron sueros pareados que se almacenaron a -70oC, se usó una prueba local de MAC-ELISA y se intentó el aislamiento del virus como en la primera. Algunos sueros procesados por el MAC-ELISA se analizaron también por el UMELISA y los estuches Dengue IgM-capture ELISA (PANBIO e IgM*ELISA anti-dengue (IPK, Cuba. Resultados: Se encontraron más casos IgM+ con la primera que con la segunda estrategia tanto con sueros agudos como convalecientes. Esto es, 61.1 vs 22% (poC que a 4oC: 17.8% vs. 4.7% (p Conclusiones: Se requiere evaluar la especificidad del estuche UMELISA considerando la alta frecuencia del dengue en casos con sospecha. El análisis de sueros pareados en la vigilancia del dengue es necesario para obtener información confiable.

  13. Implementación de la técnica en órgano aislado vascular como herramienta para la validación de plantas medicinales: Estudio del efecto vasodilatador de la Salvia scutellarioides

    Directory of Open Access Journals (Sweden)

    Jorge Hernán Ramírez

    2007-03-01

    Full Text Available Introducción: La Salvia scutellarioides es una planta con efecto antihipertensivo y diurético. En este estudio se evalúa su efecto vasodilatador con la técnica de órgano aislado vascular.Objetivos: Estandarizar la técnica de órgano aislado vascular y estudiar el efecto de S. scutellarioides en la vasoconstricción por estimulación de receptores a1.Metodología: Se colocaron anillos de aorta de rata en un equipo de baño de órganos (BIOPAC® a una temperatura de 37oC y burbujeo constante de gas carbógeno. Los cambios en la tensión se registraron con un transductor de tensión isométrica y un equipo de adquisición de datos. En la estandarización, se realizaron seis curvas de concentración-tensión con fenilefrina a las horas 1, 3 y 5, para determinar la tensión máxima (TM y la concentración efectiva 50 (EC50. Finalmente, se obtuvieron curvas de concentración-tensión para fenilefrina con S. scutellarioides a una concentración de 10 mg/dl y 20 mg/dl. Resultados: En la estandarización de la técnica no se encontraron diferencias estadísticamente significativas en la TM y la EC50 para fenilefrina en la primera, tercera y quinta horas. La administración de S. scutellariodes ocasionó una disminución concentración dependiente de la TM sin cambios en la EC50.Discusión: La técnica de órgano aislado implementada es viable hasta por cinco horas y no ocasiona desensibilización de receptores a1. La disminución de la TM por la S. scutellarioides se explica por un efecto de tipo a1 antagonista no competitivo. Se requieren estudios para aislar principios activos que permitan iniciar ensayos clínicos fase I en los seres humanos.

  14. Eficacia de la infiltración de ozono paravertebral lumbar y en puntos gatillos como coadyuvante del tratamiento en pacientes con dolor lumbar crónico y lumbociatalgia crónica en el síndrome doloroso miofascial aislado o acompañado de otras patologías

    Directory of Open Access Journals (Sweden)

    E. Silva Jiménez

    2014-02-01

    Full Text Available Introducción: Posterior al primer episodio de dolor lumbar, la recurrencia persiste durante un año o más en el 25 al 60 %, afectando a población económicamente activa, causando discapacidad y en 80 % ausentismo laboral. Objetivo: Evaluar el grado de eficacia del uso de la técnica de infiltración con ozono paravertebral lumbar y en puntos gatillos junto al tratamiento farmacológico y rehabilitador, en pacientes con dolor lumbar crónico y lumbociatalgia crónica debido al síndrome doloroso miofascial (SDM aislado o acompañado de otras patologías. Métodos: Estudio no probabilístico, de tipo experimental controlado, doble ciego. Se estudiaron 43 pacientes (22 experimental y 21 control. El grupo experimental recibió ozono paravertebral lumbar y en puntos gatillos más tratamiento farmacológico y rehabilitador. El grupo control recibió tratamiento farmacológico y rehabilitador. Se aplicó en ambos grupos la escala de EVA, Oswestry y la medición de los grados de flexión del tronco al inicio, dos y cuatro semanas posterior al comienzo del tratamiento. Resultados: La aplicación de ozono paravertebral lumbar y en puntos gatillos, junto al tratamiento farmacológico y rehabilitador, en el manejo de pacientes con dolor lumbar y lumbociatalgia crónica, comparado con solo tratamiento farmacológico y rehabilitador, resultó ser más eficaz, con significancia estadística (p < 0,05 para disminuir la intensidad del dolor (90,5 %, la incapacidad funcional (90,5 %, y aumentó los grados de flexión del tronco (85,7 % versus 40,0, 70 y 75 % respectivamente, a las cuatro semanas posteriores al inicio del tratamiento. Se evidencia mejoría en pacientes con SDM aislado o acompañado de síndrome de receso lateral, síndrome facetario, grados variables de hernia discal excepto la extrusión central. Conclusiones: La infiltración de ozono paravertebral lumbar y en puntos gatillos junto al tratamiento farmacológico y rehabilitador resultó ser m

  15. Evaluación del potencial de promoción del crecimiento vegetal de microorganismos aislados a partir de residuos de higerilla (Recinus communis) en zanahoria (daucus carota)

    OpenAIRE

    Villota, Claudia Patricia

    2013-01-01

    Tesis (Especialista en Microbiología Industrial). Universidad Católica de Manizales, 2013 Las hortalizas representan el 85% del área sembrada en Colombia, entre ellas están la arveja, tomate, cebolla de bulbo, cebolla junca, arracacha, zanahoria, cilantro, habichuela, zapallo y repollo . Al ser atacadas por plagas y enfermedades, el agricultor realiza control químico alterando de esta manera la economía, la salud y el medio ambiente. El indiscriminado uso de fertilizantes y agroquímicos en...

  16. AISLADOS BACTERIANOS CON POTENCIAL BIOFERTILIZANTE PARA PLÁNTULAS DE TOMATE

    Directory of Open Access Journals (Sweden)

    José Noh Medina

    2014-09-01

    Full Text Available En el presente trabajo se aislaron rizobacterias en diversos medios de cultivo y se resembraron en medios selectivos para estudiar su capacidad de solubilización de fosfato y de producción de ácido indolacético (AIA, con el fin de probarlas como promotoras del crecimiento vegetal en plántulas de tomate. Se obtuvieron 83 aislados bacterianos del rizoplano de plantas de chile habanero (Capsicum chinense Jacq., maíz (Zea mays y calabaza (Cucurbita pepo, de los cuales 15 sintetizaron AIA con un rango de concentración de 0.17 a 12.51 µg L-1 y 33 solubilizaron fosfato inorgánico. El uso de los aislados KCH3 y TSACH2 inoculados a las semillas de tomate, y que solubilizaron fosfato in vitro, incrementaron significativamente la biomasa de la parte aérea de las plántulas de tomate (42 y 32% respectivamente, permitiendo obtener plántulas más vigorosas que las plántulas sin inoculación de rizobacterias. Ambos aislados mostraron potencial para ser utilizados como biofertilizantes en la producción de plántulas de tomate.

  17. Aloe vera in dentistry.

    Science.gov (United States)

    Sujatha, G; Kumar, G Senthil; Muruganandan, J; Prasad, T Srinivasa

    2014-10-01

    Aloe vera is a medicinal plant which has been used for thousands of years. The health benefits of aloe vera is well known and the dental uses of this plant is multiple. Interest is gathering among researchers regarding the use of this plant. Studies have proved the antiseptic, anti inflammatory, antiviral and antifungal properties of aloe vera and the use of this plant is proved beneficial. This plant is proved to be non allergic and very good in building up the immune system. Aloe vera is gaining popularity in dentistry as it is completely natural and there is no side effects being reported with its use. This paper gives an overview of the uses of this miracle plant and its uses in dentistry.

  18. ALOS-2 initial results

    Science.gov (United States)

    Kankaku, Yukihiro; Suzuki, Shinichi; Shimada, Masanobu

    2015-10-01

    The Advanced Land Observing Satellite-2 (ALOS-2) was launched from Tanegashima Space Center by H-IIA rocket successfully on 24th May 2014. ALOS-2 carries the Phased Array type L-band Synthetic Aperture Radar-2 (PALSAR-2) as the state-of-the-art L-band SAR system which succeeds to PALSAR onboard ALOS. PALSAR-2 uses almost whole bandwidth allocated for L-band active sensor of Earth Exploration Satellites Service specified by the Radio Regulation in order to realize the high resolution observation, and also, it transmits more than 6 kW power for lower Noise Equivalent Sigma Zero using 180 TRMs driven by Gallium Nitride (GaN) amplifier which is the first use in space. Furthermore, because ALOS-2 carries the SAR system only, PALSAR-2 antenna can be mounted under the satellite body. It enables to observe right-/left-looking observation by satellite maneuvering. And the high accuracy orbit control to maintain the satellite within 500 m radius tube against the reference orbit enables high coherence for the InSAR processing. Using these new technologies, ALOS-2 has been operating to fulfill the mission requirements such as disaster monitoring and so on. This document introduces the initial result of ALOS-2 from the first year operation.

  19. Perfil fenotípico e genotípico de Staphylococcus aureus isolados de estudantes de enfermagem, 2008 Perfil fenotípico y genotípico del Staphylococcus aureus aislados de estudiantes de enfermería, 2008 Phenotypic and genotypic profile of Staphylococcus aureus isolated in nursing students, 2008

    Directory of Open Access Journals (Sweden)

    Suelen Teixeira Faria

    2011-01-01

    Full Text Available OBJETIVO: Verificar a prevalência de carreamento nasal, perfil fenotípico e genotípico de S. aureus isolados de estudantes de enfermagem. MÉTODOS: Estudo transversal, com população composta por 101 alunos, cursando as três primeiras séries do curso de graduação em Enfermagem no ano de 2008. S. aureus foi isolado de material biológico obtido dos vestíbulos nasais através de swab. A susceptibilidade à oxacilina e vancomicina foi determinada pelo teste de concentração inibitória mínima. A presença do gene MecA foi determinada pelo teste de reação em cadeia da polimerase. RESULTADOS: Verificou-se 90,1% de positividade para S. aureus. A frequência de resistência à oxacilina foi de 9,8% e todas as amostras foram sensíveis à vancomicina. A oito amostras resistentes À oxacilina apresentaram o gene MecA. CONCLUSÃO: A prevalência foi elevada. A resistência à oxacilna foi expressiva e todas as amostras foram sensíveis à vancomicina. As amostras resistentes à oxacilina carreavam o gene MecA.OBJETIVO: Verificar la prevalencia de transporte nasal, perfil fenotípico y genotípico de S. aureus aislados de estudiantes de enfermería. MÉTODOS: Estudio transversal, con población compuesta por 101 alumnos, cursando las tres primeras series del Pregrado en Enfermería en el año 2008. El S. aureus fue aislado del material biológico obtenido de los vestíbulos nasales a través de swab. La susceptibilidad a la oxacilina y vancomicina fue determinada por el test de concentración inhibitoria mínima. La presencia del gen MecA fue determinada por el test de reacción en cadena de la polimerasa. RESULTADOS: Se verificó el 90,1% de positividad para el S. aureus. La frecuencia de resistencia a la oxacilina fue de 9,8% y todas las muestras fueron sensibles a la vancomicina. Las ocho muestras resistentes a la oxacilina presentaron el gen MecA. CONCLUSION: La prevalencia fue elevada. La resistencia a la oxacilina fue expresiva y todas

  20. Estudio clínico y de resonancia magnética del rendimiento cognitivo en síndromes neurológicos aislados sugestivos de esclerosis múltiple

    OpenAIRE

    Arévalo Navinés, María Jesús

    2014-01-01

    Introducció: Històricament, encara que la presència d’alteracions cognitives en l’esclerosi múltiple (EM) ja va ser descrita per Charcot a finals del segle XIX, no ha estat fins fa pocs anys que ha cobrat especial rellevància. S’estima una freqüència del 40-60% de disfunció cognitiva en la població general que pateix aquesta malaltia i, més recentment, d’aproximadament el 27-57%, en pacients amb síndromes neurològics aïllats (SNA), segons diferents criteris de referència. Els objectius del pr...

  1. Caracterización físico-mecánica e interacciones con excipientes del D-003, una mezcla de ácidos grasos aislados de la cera de la caña de azúcar

    OpenAIRE

    Uribarri Hernández, Evangelina; González Lago, Mayra de la C.; Martínez Álvarez, Luis; Marrero Delange, David; Medina, Miriam Cora; Méndez Antolín, Ernesto J.; Laguna Granja, Abilio; González Canavaciolo, Víctor L.

    2007-01-01

    El D-003 es un nuevo producto que consiste en una mezcla de ácidos grasos de elevado peso molecular (desde C24:0 hasta C36:0), donde cada uno de ellos está en una proporción reproducible. Este producto presenta efectos antiplaquetarios y como reductor del colesterol. Como parte de los estudios de formulación del D-003 fueron llevadas a cabo la caracterización físico-mecánica y su interacción con excipientes para tabletas. El presente estudio utilizó la calorimetría diferencial de barrido (CDB...

  2. Detección y caracterización de aislados de "escherichia coli" de origen clínico y fecal en gallinas ponedoras

    OpenAIRE

    Gibert Perelló, Magdalena

    2011-01-01

    El objetivo de esta tesis ha sido realizar la caracterización de aislados de E. coli clínicos (responsables de cuadros de colibacilosis en gallinas ponedoras) y fecales. Tras llevar a cabo el aislamiento e identificación bioquímica de los aislados, se ha procedido a la determinación del serogrupo, patotipo (detección de factores de virulencia por PCR), sensibilidad a antimicrobianos y pulsotipo (mediante la técnica de Pulsed Field-Gel Electrophoresis, PFGE). Además, se ha realizado un estudio...

  3. ESTUDIO MORFOLÓGICO DEL CULTIVO A LARGO PLAZO DE FOLÍCULOS AISLADOS Y CERRADOS DE TIROIDES DE CERDO Morphological Study of Long-term culture of closed isolated pig folicles

    Directory of Open Access Journals (Sweden)

    M HERRERA

    Full Text Available La unidad morfológica y funcional de la glándula tiroides es el folículo, estructura ovoide cerrada, constituida por una capa de células cúbicas (tirocitos que encierran un lumen lleno del coloide secretado por ellas. En cultivo, tanto la estructura como la función del folículo se pierden rápidamente en las primeras 24 horas. Sin embargo, si se cultivan folículos cerrados de tiroides de rata conservan la arquitectura folicular, la morfología del tirocito y la función hasta la síntesis de hormonas tiroides de manera similar a la glándula in vivo. En este trabajo describimos el aislamiento y cultivo de folículos porcinos cerrados y su análisis morfológico. Los folículos se aíslan por digestión enzimática y disociación mecánica del parénquima tiroideo, luego se cultivan sobre agarosa con y sin hormona tirotrópica o tirotropina (1 mU/ml, TSH. El tejido de tiroides porcino obtenido tiene las mismas características de una glándula hipotiroidea in vivo, un epitelio casi plano, retículo endoplásmico rugoso (RER exiguo, complejo de Golgi (CG, y microvellosidades escasas y cortas. Los folículos cultivados sin TSH conservan la forma ovoide y el coloide en su interior, y la misma ultra-estructura del tejido in vivo, RER y CG muy escasos, pero con el tiempo de cultivo aumenta la longitud de la microvellosidades y el espesor del epitelio. En presencia de TSH el epitelio se hipertrofia desde el primer día y las cavidades foliculares se reducen considerablemente. Se demuestra que folículos cerrados de tiroides durante ocho días (d de cultivo conservan su morfología con y sin TSH. Además, estos responden al estímulo de TSH disminuyendo su cavidad folicular y aumentando el espesor del epitelio folicular.The morphological and functional unit of the thyroid gland is the follicle - an ovoid closed-structure, constituted by a layer of cubical cells (thyrocytes that lock up a full lumen of the colloid secreted by themselves. In

  4. Características hematológicas y patológicas de cerdos inoculados experimentalmente con el aislado chileno del virus síndrome respiratorio y reproductivo porcino Haematological and pathological findings of pigs experimentally inoculated with a Chilean isolate of porcine reproductive and respiratory syndrome virus

    Directory of Open Access Journals (Sweden)

    E Ramírez

    2006-01-01

    Full Text Available El presente trabajo se planteó con el fin de caracterizar las manifestaciones hematológicas y lesiones de cerdos inoculados con el aislado chileno del vPRRS. Se utilizaron 12 cerdos de 3 semanas de edad divididos en 4 grupos de 3 animales cada uno, uno de los cuales correspondió al grupo control que fueron sacrificados a los 0 días postinoculación (dpi, y los 3 grupos restantes a los cerdos inoculados que fueron sacrificados a los 7, 14 y 21 dpi. Durante cada muestreo se recolectó sangre para hemogramas y al sacrificio se determinaron las lesiones macroscópicas y se tomaron muestras para frotis de médula ósea y para histopatología. Los resultados de este estudio revelaron alteraciones hematológicas caracterizadas por descenso del volumen globular (PThe aims of this study were to characterize the haematological and bone marrow changes, gross and microscopic lesions of pigs experimentally inoculated with the Chilean isolate of porcine reproductive and respiratory syndrome virus. Twelve 3-week-old pigs were divided in 4 groups of 3, one of which corresponded to the negative control group sacrificed at 0 days post-inoculation (dpi, and the 3 remaining groups corresponded to the inoculated pigs sacrificed at 7, 14 and 21 dpi. For each sampling period blood was collected for complete haemograme and at the necropsy time gross lesions were registered and samples for both bone marrow smears and histopathology were taken. The results of this study revealed haematological alterations characterized by a significant reduction (P<0.05 in the haematocrit and a significant increase (P<0.05 in the total leukocyte count associated with an increase in the monocytes and baciliforms. The bone marrow did not show significant variations in the ratio of myeloid to erythroid cells (P0.05. At the same time, the gross lesions were mild and mainly characterized by the presence of conjunctivitis, periocular edema and a slight increase in the size of the lymph

  5. Insecticidal Activity of the Essential Oil Isolated from Azilia eryngioides (Pau Hedge et Lamond Against Two Beetle Pests Actividad Insecticida del Aceite Esencial Aislado de Azilia eryngioides (Pau Hedge et Lamond contra Dos Escarabajos Plaga

    Directory of Open Access Journals (Sweden)

    Asgar Ebadollah

    2011-09-01

    Full Text Available A large number of plant essential oils have been used against diverse insect pests since they, unlike conventional pesticides, present no risk to humans and the environment. This study was conducted to determine the toxicity of Azilia eryngioides (Pau Hedge et Lamond (Apiaceae essential oil against 1- to 7-d-old Sitophilus granarius (L. (Curculionidae and Tribolium castaneum (Herbst (Tenebrionidae adults. The essential oil was obtained from aerial parts of the plant using a Clevenger apparatus and analyzed by gas chromatography and mass spectrometry. The major constituents of the oil were α-Pinene and bornyl acetate. Fumigation bioassays revealed that A. eryngioides oil had a strong insecticidal activity on adult test insects that were exposed to 37.03, 74.07, 111.11, and 148.14 µL L-1 to estimate mean lethal time (LT50 values. Mortality increased as concentration and exposure time increased, and reached 100% at the 39-h exposure time and concentrations higher than 111.11 µL L-1. Another experiment was designed to determine the mean lethal concentration at the 24-h exposure time (LC50-, and these values indicated that S. granarius was more susceptible than T. castaneum. It can be concluded that the essential oil of A. eryngioides has potential against two stored-product pests, S. granarius and T. castaneum.Una gran cantidad de aceites esenciales de plantas se han utilizado como agentes de control biológico contra diversos insectos plaga, ya que no presentan riesgo para los seres humanos y el medio ambiente, a diferencia de los pesticidas convencionales. Se determinó la toxicidad del aceite esencial de Azilia eryngioides (Pau Hedge et Lamond (Apiaceae contra adultos de 1 a 7 días de edad de Sitophilus granarius (L. (Curculionidae y Tribolium castaneum (Herbst (Tenebrionidae. El aceite esencial se obtuvo de las partes aéreas de la planta utilizando un aparato de Clevenger y se analizó por cromatografía de gases por espectrometría de

  6. Clonaje y caracterización molecular in silico de un transcrito de fosfolipasa A2 aislado del veneno de la serpiente peruana Lachesis muta Molecular cloning and characterization in silico of phospholipase A2 transcripto isolated from Lachesis muta peruvian snake venom

    Directory of Open Access Journals (Sweden)

    Karim L. Jimenez

    2010-12-01

    Full Text Available Objetivo. Aislar y caracterizar in silico un transcrito del gen de fosfolipasa A2 (PLA2 aislado del veneno de Lachesis muta de la Amazonía peruana. Materiales y métodos. Se amplificó el transcrito del gen sPLA2 mediante la técnica de RT-PCR a partir de RNA total utilizando cebadores específicos, el producto de DNA amplificado se insertó en el vector pGEM para su posterior secuenciación. Mediante análisis bioinformático de la secuencia nucleotídica se determinó un marco de lectura abierta de 414 nucleótidos que codifica 138 aminoácidos, incluyendo16 aminoácidos del péptido señal, el peso molecular y el pI fueron de 13 976 kDa y 5,66 respectivamente. Resultados. La secuencia aminoacídica denominada Lm-PLA2- Perú, contiene Asp49, así como Tyr-28, Gly-30, Gly-32, His-48, Tyr52, Asp99 importantes para la actividad enzimática. La comparación de Lm-PLA2-Perú con las secuencias aminoacídicas de los bancos de datos mostró 93% de similitud con las sPLA2 de Lachesis stenophrys y más del 80% con otras sPLA2 de venenos de la familia Viperidae. El análisis filogenético de la secuencia nucleotídica del transcrito del gen sPLA2 indica que Lm-PLA2-Perú se agrupa con otras sPLA2 [Asp49] ácidas previamente aisladas del veneno de Bothriechis schlegelii con un 89% de identidad. El modelaje tridimensional de Lm-PLA2-Perú, presenta una estructura característica de sPLA2 del Grupo II formada por tres hélices-α, una lámina-β, una hélice corta y un lazo de unión con calcio. Conclusión. La secuencia nucleotídica corresponde al primer transcripto del gen de PLA2 clonado a partir del veneno de la serpiente Lachesis muta, que habita en la selva del Perú.Objective. Isolate and characterize in silico gene phospholipase A2 (PLA2 isolated from Lachesis muta venom of the Peruvian Amazon. Material and methods. Technique RT-PCR from total RNA was using specific primers, the amplified DNA product was inserted into the pGEM vector for

  7. Final report on the safety assessment of AloeAndongensis Extract, Aloe Andongensis Leaf Juice,aloe Arborescens Leaf Extract, Aloe Arborescens Leaf Juice, Aloe Arborescens Leaf Protoplasts, Aloe Barbadensis Flower Extract, Aloe Barbadensis Leaf, Aloe Barbadensis Leaf Extract, Aloe Barbadensis Leaf Juice,aloe Barbadensis Leaf Polysaccharides, Aloe Barbadensis Leaf Water, Aloe Ferox Leaf Extract, Aloe Ferox Leaf Juice, and Aloe Ferox Leaf Juice Extract.

    Science.gov (United States)

    2007-01-01

    Plant materials derived from the Aloe plant are used as cosmetic ingredients, including Aloe Andongensis Extract, Aloe Andongensis Leaf Juice, Aloe Arborescens Leaf Extract, Aloe Arborescens Leaf Juice, Aloe Arborescens Leaf Protoplasts, Aloe Barbadensis Flower Extract, Aloe Barbadensis Leaf, Aloe Barbadensis Leaf Extract, Aloe Barbadensis Leaf Juice, Aloe Barbadensis Leaf Polysaccharides, Aloe Barbadensis Leaf Water, Aloe Ferox Leaf Extract, Aloe Ferox Leaf Juice, and Aloe Ferox Leaf Juice Extract. These ingredients function primarily as skin-conditioning agents and are included in cosmetics only at low concentrations. The Aloe leaf consists of the pericyclic cells, found just below the plant's skin, and the inner central area of the leaf, i.e., the gel, which is used for cosmetic products. The pericyclic cells produce a bitter, yellow latex containing a number of anthraquinones, phototoxic compounds that are also gastrointestinal irritants responsible for cathartic effects. The gel contains polysaccharides, which can be acetylated, partially acetylated, or not acetylated. An industry established limit for anthraquinones in aloe-derived material for nonmedicinal use is 50 ppm or lower. Aloe-derived ingredients are used in a wide variety of cosmetic product types at concentrations of raw material that are 0.1% or less, although can be as high as 20%. The concentration of Aloe in the raw material also may vary from 100% to a low of 0.0005%. Oral administration of various anthraquinone components results in a rise in their blood concentrations, wide systemic distribution, accumulation in the liver and kidneys, and excretion in urine and feces; polysaccharide components are distributed systemically and metabolized into smaller molecules. aloe-derived material has fungicidal, antimicrobial, and antiviral activities, and has been effective in wound healing and infection treatment in animals. Aloe barbadensis (also known as Aloe vera)-derived ingredients were not toxic

  8. Aloe vera: A short review

    Directory of Open Access Journals (Sweden)

    Surjushe Amar

    2008-01-01

    Full Text Available Aloe vera is a natural product that is now a day frequently used in the field of cosmetology. Though there are various indications for its use, controlled trials are needed to determine its real efficacy. The aloe vera plant, its properties, mechanism of action and clinical uses are briefly reviewed in this article.

  9. Aloe vera: Plant of Immortality

    Directory of Open Access Journals (Sweden)

    Sikarwar Mukesh. S.

    2010-02-01

    Full Text Available The Egyptians called Aloe the “Plant of Immortality” because it can live and even bloom without soil. Aloe has been used medicinally since at least the first century C.E. and continues to be used extensively worldwide.

  10. FLAVONOIDES AISLADOS DE LAS INFLORESCENCIAS DE Piper hispidum Kunth (PIPERACEAE Y DERIVADOS ACETILADOS

    Directory of Open Access Journals (Sweden)

    Luis Enrique Cuca

    2009-04-01

    Full Text Available A partir del extracto etanólico de las inflorescencias de la especie Piper hispidum Kunth (Piperaceae fueron aislados tres flavonoides: 5-hidroxi-7-metoxiflavanona, 5-hidroxi-4’,7-dimetoxiflavanona y 2’,4’,6’-trimetoxidihidrochalcona. De las flavanonas aisladas fueron obtenidos los  derivados acetilados: 5-acetoxi-7-metoxiflavanona y  5-acetoxi-4’,7-dimetoxiflavanona. Las estructuras fueron elucidadas empleando técnicas espectroscópicas y por comparación con datos de literatura. Los compuestos naturales y sus derivados fueron sometidos al bioensayo de letalidad frente a Artemia salina. El flavonoide 5-hidroxi-7-metoxiflavanona presenta la mayor actividad tóxica frente a los microcrustaceos CL50 1.8 µg/ml.

  11. Aloe Vera for Tissue Engineering Applications

    Directory of Open Access Journals (Sweden)

    Shekh Rahman

    2017-02-01

    Full Text Available Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

  12. Aloe Vera for Tissue Engineering Applications.

    Science.gov (United States)

    Rahman, Shekh; Carter, Princeton; Bhattarai, Narayan

    2017-02-14

    Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

  13. Precise Orbit Determination for ALOS

    Science.gov (United States)

    Nakamura, Ryo; Nakamura, Shinichi; Kudo, Nobuo; Katagiri, Seiji

    2007-01-01

    The Advanced Land Observing Satellite (ALOS) has been developed to contribute to the fields of mapping, precise regional land coverage observation, disaster monitoring, and resource surveying. Because the mounted sensors need high geometrical accuracy, precise orbit determination for ALOS is essential for satisfying the mission objectives. So ALOS mounts a GPS receiver and a Laser Reflector (LR) for Satellite Laser Ranging (SLR). This paper deals with the precise orbit determination experiments for ALOS using Global and High Accuracy Trajectory determination System (GUTS) and the evaluation of the orbit determination accuracy by SLR data. The results show that, even though the GPS receiver loses lock of GPS signals more frequently than expected, GPS-based orbit is consistent with SLR-based orbit. And considering the 1 sigma error, orbit determination accuracy of a few decimeters (peak-to-peak) was achieved.

  14. Estabilidad a la congelación descongelación de emulsiones o/w preparadas con aislados de soja nativos y desnaturalizados con diferente solubilidad

    Directory of Open Access Journals (Sweden)

    Gonzalo Palazolo

    2011-05-01

    Full Text Available En este trabajo se evaluó la estabilidad a la congelación de emulsiones modelo aceite en agua (o/w preparadas con cuatro aislados de soja: dos nativos (ASN-1 y ASN-2, de similar composición pero diferente solubilidad, >90% y ~75%, respectivamente y dos desnaturalizados (ASD-1 y ASD-2, resultantes de calentar los respectivos aislados nativos (90 ‹C, 5 minutos. Las emulsiones preparadas con dispersiones acuosas al 2% p/v de ASN o ASD y aceite de girasol refinado (ƒ³ =0,25, se congelaron a -20 ‹C por 24 horas y luego se descongelaron a 20 ‹C. La estabilidad se evaluo a partir de medidas de distribución de tamano de partícula (difraccion laser y aceite liberado (AL, metodo de dilucion del colorante. Las emulsiones preparadas con ASN-2 y ASD-2 resultaron muy inestables despues de ser congeladas y descongeladas, resultadoque se evidenció por el aumento del tamano de partícula y un AL>25%. Al usar el aislado ASN-1 de mayor solubilidad, las emulsiones fueron mas estables y se observó un marcado incremento de la estabilidad cuando sus proteínas eran desnaturalizadas (ASD-1, no observándose prácticamente coalescencia ni aceite separado. En conclusión, la solubilidad y la desnaturalización proteíca de los aislados de soja son factores decisivos en la estabilidad de emulsiones frente a la congelación-descongelación.

  15. Lignanos diarildimetilbutanos y otros constituyentes aislados de Nectandra turbacensis (kunth nees (lauraceae

    Directory of Open Access Journals (Sweden)

    Víctor Macías Villamizar

    2014-01-01

    Full Text Available Ocho compuestos conocidos fueron aislados del extracto etanólico de corteza de Nectandra turbacensis (Kunth Nees (Lauraceae. Estos fueron identificados como ácido meso-dihidroguayarético 1, ácido treo-dihidroguayarético 2, sauriol B 3, y treo-austrobailignano-6 4; vitexina (8-C-β-D-glucopiranosil-5,7,4’-trihidroxiflavona 5; estigmast-4-en-3-ona 6 y la mezcla sitosterol 7 / estigmasterol 8. Las estructuras de los compuestos fueron elucidadas por métodos espectroscópicos, que incluyeron técnicas de RMN en 1D y 2D, CG/EM y por comparación de los datos espectroscópicos, reportados en la literatura de compuestos relacionados. Este es el primer reporte de la presencia de este tipo de compuestos en la especie. Se describen también las implicaciones quimiotaxonómicas; relacionadas con la presencia frecuente de lignanos en especies del género Nectandra.

  16. Nuevo alcaloide oxoaporfínico y otros constituyentes químicos aislados de Pleurothyrium cinereum (Lauraceae.

    Directory of Open Access Journals (Sweden)

    Ericsson Coy

    2009-04-01

    Full Text Available Del extracto etanólico de las hojas de Pleurothyrium cinereum (Lauraceae fue aislado el nuevo alcaloide oxoaporfínico oxiprenilado 1,2-metilendioxi-9,10-dimetoxi-3-isopreniloxi-7H-dibenzo[de,g]quinolin-7-ona (Pleurotirina 1, el cual fue purificado por  métodos cromatográficos y cuya elucidación estructural se realizó mediante técnicas espectroscópicas (RMN-1H, RMN-13C, RMN-2D y EM. Junto al alcaloide 1 fueron aislados los compuestos thalicminina 2, ácido ent-kaurenóico 3, alloxantoxiletina 4, xantiletina 5, dihydroflavokawina B 6, 3’-metoxi-3,4-metilendioxi-4’,7-epoxi-nor-8.5’-neolignan-7.8’-dieno 7 y friedelina 8, los cuales se reportan por primera vez para la especie y para el género.

  17. Aloe in Angola (Asphodelaceae: Alooideae

    Directory of Open Access Journals (Sweden)

    R. R. Klopper

    2009-12-01

    Full Text Available Botanical exploration of Angola was virtually impossible during the almost three-decade-long civil war. With more areas becoming accessible, there is, however, a revived interest in the flora of this country. A total of 27 members of the genus Aloe L. have been recorded for Angola. It is not unlikely that new taxa will be discovered, and that the distribution ranges of others will be expanded now that botanical exploration in Angola has resumed. This manuscript provides a complete taxonomic treatment of the known Aloe taxa in Angola. It includes, amongst other information, identification keys, descriptions and distribution maps.

  18. Caracterización bioquímica y molecular de aislados de Klebsiella pneumoniae resistente a antimicrobianos

    Directory of Open Access Journals (Sweden)

    Laura Margarita Castañeda

    2000-02-01

    diferentes aislados bacterianos resistentes a antibióticos, son insuficientes para discriminar un posible brote de infección nosocomial, siendo necesaria la utilización de técnicas basadas en el estudio genotípico, que logren establecer diferencias entre los aislados.

    El análisis del perfil de plásmidos es de mucha aplicabilidad, ya que permite una evaluación epidemiológica útil para determinar la diferencia o la semejanza entre los aislados sometidos a estudio. Sin embargo, no es suficiente para determinar el origen clonal de un brote y se requiere un método que permita una caracterización mas precisa de las cepas. Para este fin, se utiliza el análisis del DNA genómico, cortado con endonucleasas de restricción con baja frecuencia de corte, por medio de la electroforesis de campo pulsado (PFGE, que ha mostrado ser una buena herramienta epidemiológica, debido a su alta reproducibilidad y a su poder discriminatorio para determinar el origen clonal en varias especies bacterianas incluyendo Klebsiella pneunoniae, Este proyecto pretende determinar y caracterizar el comportamiento genético de esta resistencia en las cepas de Klebsiella pneumoniae circulantes en nuestro medio, lo que permitirá plantear estrategias epidemiológicas en el manejo y prevención de las diferentes infecciones debidas a este microorganismo.

    Objetivo general: Caracterizar bioquímica y molecularmente aislados de Klebsiella pneumoniae resistentes a antimicrobianos.

    Objetivos específicos: 1. Biotipificar los aislados de Klebsiella pneumoniae. 2. Determinar la susceptibilidad de los aislados, incluyendo la detección de la producci

  19. Parámetros cinéticos como herramienta para la caracterización de aislados de Aspergillus sección Nigri

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    María Silvina Sobrero

    2013-12-01

    Full Text Available Las especies de Aspergillus dentro de la sección Nigri son importantes en procesos biotecnológicos, así como en el biodeterioro. Bajos condiciones de cultivo controladas, la velocidad de crecimiento es una característica de las especies de hongos y algunos autores utilizan la medida del diámetro de las colonias como herramienta de identificación. El objetivo de este estudio fue determinar si la velocidad de crecimiento obtenida por la Microbiología Predictiva puede ser utilizada como herramienta para la caracterización de aspergilos negros. Se construyeron las curvas de crecimiento de 5 aislados de aspergilos negros obtenidos de alimentos y ambiente de industria láctea. Estos aislados, también fueron estudiados por sus características morfológicas y estudios de microscopía electrónica. Se identificaron como A. awamori, A. niger (2 aislados, A. foetidus y A. carbonarius. Las velocidades máximas de crecimiento en medio Agar Extracto de Malta a 25 ºC fueron: 3,51; 4,85; 4,52; 12,73; y 5,84 mm/día, respectivamente. A foetidus fue el único que presentó fase de latencia y alcanzó el mayor radio. Con la ayuda de la Microbiología Predictiva se pudieron separar A. awamori de A. foetidus, perolas diferencias no fueron significativas (p > 0,05 para los otros aislados. Los caracteres morfológicos permitieron diferenciar a A. carbonarius. Los datos cinéticos por sí solos no fueron suficientes para diferenciar especies cercanas.

  20. Parámetros cinéticos como herramienta para la caracterización de aislados de Aspergillus sección Nigri

    Directory of Open Access Journals (Sweden)

    María Silvina Sobrero

    2013-12-01

    Full Text Available Las especies de Aspergillus dentro de la sección Nigri son importantes en procesos biotecnológicos, así como en el biodeterioro. Bajos condiciones de cultivo controladas, la velocidad de crecimiento es una característica de las especies de hongos y algunos autores utilizan la medida del diámetro de las colonias como herramienta de identificación. El objetivo de este estudio fue determinar si la velocidad de crecimiento obtenida por la Microbiología Predictiva puede ser utilizada como herramienta para la caracterización de aspergilos negros. Se construyeron las curvas de crecimiento de 5 aislados de aspergilos negros obtenidos de alimentos y ambiente de industria láctea. Estos aislados, también fueron estudiados por sus características morfológicas y estudios de microscopía electrónica. Se identificaron como A. awamori, A. niger (2 aislados, A. foetidus y A. carbonarius. Las velocidades máximas de crecimiento en medio Agar Extracto de Malta a 25 ºC fueron: 3,51; 4,85; 4,52; 12,73; y 5,84 mm/día, respectivamente. A foetidus fue el único que presentó fase de latencia y alcanzó el mayor radio. Con la ayuda de la Microbiología Predictiva se pudieron separar A. awamori de A. foetidus, pero las diferencias no fueron significativas (p > 0,05 para los otros aislados. Los caracteres morfológicos permitieron diferenciar a A. carbonarius. Los datos cinéticos por sí solos no fueron suficientes para diferenciar especies cercanas.

  1. ALOE EN VENEZUELA: DE LA CADENA DE VALOR AL DISTRITO INDUSTRIAL

    Directory of Open Access Journals (Sweden)

    Henri Jesús Piña Zambrano

    2010-01-01

    Full Text Available Desde la perspectiva del estudio de los conglomerados, se contrastaron las aportaciones teóricas acerca de los conglomerados y el funcionamiento y desempeño de la cadena del aloe en el Estado Falcón (Venezuela, partiendo de la hipótesis de que esta cadena ha seguido un patrón evolutivo sustentado en el legado histórico de recursos que han favorecido la siembra y explotación del aloe en el estado. Sin embargo, se considera un escaso aprovechamiento de este patrimonio como estrategia para conformar un eficiente y competitivo conglomerado agroindustrial. La investigación se sustenta en estudios realizados entre 2003 y 2007 en las zonas productoras del aloe. Se identifican en la cadena los elementos típicos de un conglomerado (proximidad geográfica, redes, innovación e institucionalidad bajo un patrón organizativo que no promueve la consolidación del conglomerado. En la cadena del aloe no termina de crearse la atmósfera industrial necesaria para iniciar la districtualización de esta actividad productiva.

  2. Gérmenes patógenos aislados en niños con infecciones respiratorias a repetición

    Directory of Open Access Journals (Sweden)

    Yoenny Peña García

    2015-01-01

    Full Text Available Las infecciones respiratorias agudas son la causa más frecuente de morbilidad y de elevada mortalidad en el mundo, particularmente en los países en desarrollo. Se realizó un estudio descriptivo de corte transversal, para caracterizar a los niños con infecciones respiratorias a repetición, según los gérmenes patógenos aislados, en el círculo infantil “Flores de la Vida” del municipio de Puerto Padre, en el período de abril a mayo de 2014. Se trabajó con los niños de los salones de segundo, tercero y cuarto año de vida, distribuidos según edad y sexo, a los que se les identificaron los gérmenes patógenos aislados en exudados nasofaríngeos. La información se obtuvo del departamento de microbiología del Centro Municipal de Higiene y Epidemiología. Se obtuvieron los siguientes resultados: el grupo de edad más frecuente fue el de dos años, que representó el 45 % de la muestra y se correspondió con la media de la edad. El 62,5 % de la muestra de estudio correspondió al sexo masculino. Los gérmenes patógenos aislados con mayor frecuencia fueron: el Streptococcus pneumoneae, para un 83,9 %, el Streptococcus B hemolítico, para un 7,1 % y el Haemophillus inflienzae para un 5,4 %

  3. Revisión de la aloe vera (Barbadensis Miller) en la dermatología actual Revision Of Aloe Vera (Barbadensis Miller) In Actual Dermatology

    OpenAIRE

    2009-01-01

    El Aloe vera es una planta de uso popular en el tratamiento de algunas enfermedades de la piel y de aplicación frecuente en la cosmetología. Forma parte de diversas cremas o geles, siendo usado como antiinflamatorio y reconstituyente del tejido epitelial. Aunque se conocen varios mecanismos de acción e indicaciones clínicas, se debe realizar una revisión de las mismas para su uso, avalados por ensayos controlados para determinar su eficacia real.The Aloe vera is a popular plant used in the tr...

  4. NUEVOS AISLADOS DE Gluconacetobacter diazotrophicus EN CULTIVOS DE IMPORTANCIA ECONÓMICA PARA CUBA

    Directory of Open Access Journals (Sweden)

    B. Dibut

    2005-01-01

    Full Text Available En los últimos años, un llamado importante para los microbiólogos del suelo ha sido el estudio de microorganismos endófitos que se asocian con plantas superiores, con el consiguiente beneficio sobre los cultivos. En este trabajo, se ofrecen los resultados sobre el aislamiento y la distribución de Gluconacetobacter diazotrophicus en cultivos de importancia económica para Cuba. La bacteria se aisló a partir de filtrados y secciones de diferentes órganos del vegetal dispuestos sobre medios de cultivo LGI-P, donde se comprobó el crecimiento característico a las 96 h de incubación a 320C de temperatura, obteniéndose finalmente 22 aislados a partir de diez especies cultivables. Experimentos de dinámica poblacional desarrollados en condiciones de invernadero mediante diseño completamente aleatorizado permitieron cuantificar el microorganismo en hojas, tallos y raíces de maíz, boniato, yuca, malanga y caña de azúcar, con poblaciones que oscilan entre 1.8 x 102-2.3 x 107 células por gramo de tejido fresco. Las poblaciones celulares más altas se detectaron en las hojas, seguido de los tallos y por último las raíces o tubérculos. La respuesta favorable de ocho cultivos a la inoculación de la bacteria, esta vez crecida en medio de cultivo SG e incubada a 320C durante 72 h, permite plantear la potencialidad que este microorganismo presenta como biofertilizante. Se informa por primera vez la presencia de la Gluconacetobacter diazotrophicus en cinco especies de plantas pertenecientes cada una a diferentes familias botánicas.

  5. Primer registro de Allomyces neomoniliformis (Chytridiomycota y Dictyuchus missouriensis (Oomycota aislados de un suelo agrícola (Buenos Aires, Argentina

    Directory of Open Access Journals (Sweden)

    Mónica M. Steciow

    2001-01-01

    Full Text Available Allomyces neomoniliformis y Dictyuchus missouriensis fueron aislados de un suelo agrícola conteniendo materia orgánica (hojas, raíces y tallos en Argentina. Ambas especies son citadas por primera vez para la Argentina y por segunda vez para Sudamérica, siendo el hallazgo más austral para el hemisferio occidental. Constituye el segundo aislamiento en Argentina de especies del género Allomyces y Dictyuchus. Se realizan consideraciones acerca de la distribucion de ambas especies

  6. Evaluación de microorganismos aislados de gallinaza por su potencial para el biocontrol de fusarium (f. oxysporum) en plántulas de uchuva (physalis peruviana).

    OpenAIRE

    Rodríguez Amézquita, Jorge Enrique; Velandia Monsalve, Jorge; Viteri Rosero, Silvio Edgar

    2011-01-01

    En Colombia, las pérdidas económicas ocasionadas por Fusarium oxysporum en el cultivo de uchuva son considerables. Se evaluaron hongos y bacterias aislados de 2 fuentes de gallinaza, su potencial como agentes de biocontrol de este patógeno. La evaluación se realizó en cajas de Petri con PDA para lo cual se colocó en el centro de las mismas, un disco de 5 mm de diámetro colonizado por el patógeno y a 3 cm del centro, sobre los ejes horizontal y vertical, cada uno de los aislamientos de la gall...

  7. Películas biodegradables y comestibles desarrolladas en base a aislado de proteínas de suero lácteo : estudio de dos métodos de elaboración y del uso de sorbato de potasio como conservador

    Directory of Open Access Journals (Sweden)

    Daniela Escobar

    2011-05-01

    Full Text Available Las películas se elaboraron a partir de soluciones de aislado de proteínas de suero lácteo (WPI, utilizando glicerol como plasticante y con el agregado o no de sorbato de potasio como conservador. Los métodos utilizados fueron el de moldeo por compresión a 140 ºC y 1 MPa y el método de casting a 23 ºC y 55 % de humedad relativa. El método de moldeo por compresión presenta la ventaja de involucrar menos tiempo y menos incertidumbre para la formación de las películas comparado con el método de casting. Por otro lado, mejora las propiedades mecánicas de las películas aumentando su stress máximo y su elongación y disminuyendo su permeabilidad al vapor de agua y su solubilidad.Palabras clave: Moldeo por compresión, casting, fuerza, stress, elongación, módulo de Young.AbstractThe films were made from whey protein isolate (WPI solutions using glycerol as plasticizer and with or without the use of potassium sorbate as antimicrobial. The methods used were compression molding at 140 ºC and 1 MPa and the casting method at 23º C and 55 % of humidity. The compression molding method has the advantage of involving less time and uncertainty for the film forming than the casting method. On the other hand, it improves the mechanical properties bringing  films with higher tensilestrength and elongation, and less water vapor permeability and solubility.Keywords: Compression molding, casting, force, tensile strength, elongation, elastic modulus.

  8. Virus de la Enfermedad Infecciosa de la Bolsa de Fabricio: Relaciones antigénicas de aislados cubanos y chilenos Infectious Bursal Disease Virus: antigenic interrelationships between

    Directory of Open Access Journals (Sweden)

    J Noda

    2005-01-01

    Full Text Available La Enfermedad Infecciosa de la Bolsa (EIB sigue afectando la industria avícola por la aparición de variantes patogénicas y antigénicas del virus, originadas en la permanente evolución del virus producto de la presión inmunológica por el uso intensivo de vacunas. La caracterización antigénica de los virus de campo resulta esencial para la aplicación de vacunas más adecuadas en el control de la enfermedad. En este trabajo se determinaron las relaciones antigénicas de aislados cubanos y chilenos, obtenidos de poblaciones de pollos vacunados con el virus de la EIB. Los aislados virales se adaptaron a cultivos celulares y se obtuvieron antisueros monoespecíficos de cada uno de ellos y de una cepa de referencia. Las relaciones se establecieron por virusneu-tralización cruzada utilizando nueve aislados cubanos (BD, BL, 35/95, 29/96, 118/96, BF2, BF8, BF9 y 70/98, tres chilenos (G1, G2 y G4 y una cepa de referencia del serotipo 1 (Lukert. Los aislados cubanos y chilenos se adaptaron eficientemente a cultivos de fibroblastos de embrión de pollo (con excepción de BF3 y G3. Además, los aislados cubanos se adaptaron a células VERO, presentando mayores títulos infectivos en fibroblastos de embrión de pollo que en esta línea celular. Los resultados de la seroneutralización cruzada mostraron entre los aislados cubanos una relación mayor a un 80% y entre éstos y la cepa de referencia mayor de un 70%, de igual modo con los aislados chilenos G1 y G4 (mayor de 77%. El aislado G2 presentó diferencias antigénicas consideradas menores con los aislados cubanos BL, 35/95 y 29/96 (­ 69%. Ninguno de los aislados mostró relaciones antigénicas inferiores al 30% con la cepa de referencia del serotipo 1, por lo tanto no corresponden a cepas variantesInfectious Bursal Disease (IBD is still affecting the poultry industry through the appearance of pathogenic and antigenic variations of the virus. This is due to its permanent evolution as a

  9. An evaluation of the biological and toxicological properties of Aloe barbadensis (miller), Aloe vera.

    Science.gov (United States)

    Boudreau, Mary D; Beland, Frederick A

    2006-04-01

    Aloe barbadensis (Miller), Aloe vera, has a long history of use as a topical and oral therapeutic. The plant is the source of two products, gel and latex, which are obtained from its fleshy leaves. Aloe vera products contain multiple constituents with potential biological and toxicological activities, yet the active components elude definition. Ingestion of Aloe vera is associated with diarrhea, electrolyte imbalance, kidney dysfunction, and conventional drug interactions; episodes of contact dermatitis, erythema, and phototoxicity have been reported from topical applications. This review examines the botany, physical and chemical properties, and biological activities of the Aloe vera plant.

  10. Los pueblos indígenas aislados de la frontera brasil-perú: nuevas amenazas y desafíos para la garantía de sus derechos

    OpenAIRE

    Hilton S. Nascimento

    2011-01-01

    La frontera entre Brasil y Perú está habitada por la segunda mayor concentración de pueblos indígenas aislados del mundo, grupos sociales con alto grado de autonomía que evitan el contacto permanente con las sociedades de su entorno. A partir del año 2000 la región comenzó a ser centro de políticas orientadas a favorecer el acceso de sus recursos naturales (madera, petróleo y potencial hidroeléctrico) al mercado mundial y a construir obras de infraestructura que son parte del proyecto de inte...

  11. Daya Hambat Ekstrak Aloe Vera terhadap pertumbuhan Staphylococcus Aureus

    OpenAIRE

    Rahmat, drg.Sp,Pros

    2011-01-01

    Dari hasil penelitian , maka dapat disimpulkan bahwa ekstrak Aloe Vera dapat menghambat pertumbuhan bakteri Stafhylococcus aureus, dan kadar hambat minimal ekstrak Aloe Vera adalah pada konsentrasi 25%. Tujuan Penelitan Ini adalah untuk mengetahui efektifitas ekstrak Aloe vera dalam menghambat pertumbuhan bakteri Stafhylococcus aureus dan daya hambat menimal, (DHM) terhadap pertumbuhan bakteri tersebut. Metode yang digunakan adalah pertumbuhan ekstrak Aloe vera, penegnceran ekstrak , pemur...

  12. Aloe vera COMO SUSTRATO PARA EL CRECIMIENTO DE Lactobacillus plantarum y L. casei

    OpenAIRE

    B. A González; R. Domínguez-Espinosa; B. R. Alcocer

    2008-01-01

    En este estudió se determinó el efecto del uso de jugo de Aloe vera (sábila) como sustrato principal de fermentación para obtener cultivos de alta concentración de células viables de dos bacterias con actividad probiótica: Lactobacillus plantarum (NCIMB 11718) y Lactobacillus casei (NRRL -1445). Se determinó la velocidad específica de crecimiento (u) de cada microorganismo en medios con diferentes concentraciones de Aloe vera comparándolos con aquellos obtenidos en cultivos crecidos en medio ...

  13. EVALUACION DEL EFECTO INMUNOESTIMULANTE DE COMPUESTOS AISLADOS DE ESPECIES DEL GENERO HELIOTROPIUM EN SALMONIDOS

    OpenAIRE

    2012-01-01

    Uno de los mayores problemas en las pisciculturas durante estas últimas décadas ha sido el aumento en el número de enfermedades ocasionadas principalmente por patógenos intracelulares y que aún no cuenta con tratamientos efectivos que se traduzcan en un control adecuado. Debido a esto han surgido una serie de estrategias para controlar las enfermedades infecciosas en la acuicultura entre otras cambios en la nutrición, desarrollo de vacunas y el uso de inmunoestimulantes. A la fecha, las...

  14. Aloe vera in dermatology: a brief review.

    Science.gov (United States)

    Feily, A; Namazi, M R

    2009-02-01

    Aloe vera Linne or aloe barbadensis Miller is a succulent from the Aloe family (400 different species), a tropical plant which is easily grown in hot and dry climates and widely distributed in Asia, Africa and other tropical areas. The use of aloe vera is being promoted for a large variety of conditions. The aim of this systematic review was to summarize all dermatology-oriented in vitro and in vivo experiments and clinical trials on aloe vera preparations. Extensive literature search were carried out to identify all in vitro and in vivo studies as well as clinical trials on the subject. Data were extracted from these in a predefined standardized manner. Forty studies were located. The results suggest that oral administration of aloe vera in mice is effective on wound healing, can decrease the number and size of papillomas and reduce the incidence of tumors and leishmania parasitemia by >90% in the liver, spleen, and bone marrow. Topical application of aloe vera is not an effective prevention for radiation-induced injuries and has no sunburn or suntan protection. It can be effective for genital herpes, psoriasis, human papilloma virus, seborrheic dermatitis, aphthous stomatitis, xerosis, lichen planus, frostbite, burn, wound healing and inflammation. It can also be used as a biological vehicle and an anti-microbial and antifungal agent and also as a candidate for photodynamic therapy of some kinds of cancer. Even though there are some promising results with the use of aloe vera for diverse dermatologic conditions, clinical effectiveness of oral and topical aloe vera is not sufficiently and meticulously explored as yet.

  15. Estudio anatomopatológico de aislados de Leptospira spp., provenientes de Nicaragua en Mesocricetus auratus como biomodelo

    Directory of Open Access Journals (Sweden)

    Willian Jirón T.

    2013-08-01

    Full Text Available Objetivo. El objetivo de este trabajo fue caracterizar en el biomodelo Mesocricetus auratus la sintomatología y lesiones anatomopatológicas que provocan 5 aislados clínicos de Leptospira spp., provenientes de Nicaragua. Materiales y métodos. Con este fin se inocularon 50 hámster por vía i.p con 1mL del cultivo de cada una de las cepas en fase exponencial teniendo una concentración celular de 7.5 x 106 leptospira/mL (10 animales por cepa, evaluándose signos de la enfermedad, mortalidad durante 14 días, lesiones anatomopatológicas macroscópicas y microscópicas mediante tinción con hematoxilina-eosina y tinción de Warthyn Starryn. Resultados. Todas las cepas presentaron alta mortalidad, mostrando un cuadro tanto clínico, como lesional característico de la infección experimental. Además, causaron la muerte al 100% de los animales entre el tercer y décimo día postinfección. En el estudio anatomopatológico la cepa del serogrupo Ballum y la del serogrupo Pomona produjeron focos de hemorragias específicamente en el riñón y pulmones. De forma similar ocurrió una congestión hepática y renal, mientras que la hemorragia renal fue observada con mayor frecuencia en la cepa del serogrupo Pomona, diferenciándose del resto de las cepas que mostraron esta lesión con menos frecuencia. Conclusiones. Este trabajo permitió una mayor caracterización de estas cepas siendo utilizadas como futuras candidatas vacunales frente a una nueva epidemia de Leptospirosis en Nicaragua.

  16. FLAVONOIDES AISLADOS DE LAS INFLORESCENCIAS DE Piper hispidum Kunth (PIPERACEAE Y DERIVADOS ACETILADOS

    Directory of Open Access Journals (Sweden)

    Erika Plazas

    2009-04-01

    Full Text Available En el articulo "FLAVONOIDES AISLADOS DE LAS INFLORESCENCIAS DE Piper hispidum Kunth (PIPERACEAE Y DERIVADOS ACETILADOS" de Erika A. Plazas G., Luis E. Cuca S., Wilman A. Delgado A Rev Colomb. Quim., 2008, 37(2: 135-144 falto incluir lo siguiente.

  17. Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera

    DEFF Research Database (Denmark)

    Grace, Olwen Megan; Buerki, Sven; Symonds, Matthew RE;

    2015-01-01

    Background: Aloe vera supports a substantial global trade yet its wild origins, and explanations for its popularity over 500 related Aloe species in one of the world’s largest succulent groups, have remained uncertain. We developed an explicit phylogenetic framework to explore links between...... the rich traditions of medicinal use and leaf succulence in aloes. Results: The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major...... succulence among aloes has yielded new explanations for the extraordinary market dominance of Aloe vera. The industry preference for Aloe vera appears to be due to its proximity to important historic trade routes, and early introduction to trade and cultivation. Well-developed succulent leaf mesophyll tissue...

  18. Diseño de la Formulación de un Material de Empaque Flexible y Comestible a Base de Aislado Proteínico de Suero

    Directory of Open Access Journals (Sweden)

    Carlos Regalado González

    2005-01-01

    Full Text Available En la actualidad es muy común resguardar alimentos con empaques de plástico de naturaleza sintética. Actualmente alrededor de un 30% en peso de los desechos municipales corresponden a empaques sintéticos, lo que sugiere la necesidad de crear alternativas ecológicas de empaque, específicamente el diseño y elaboración de películas biodegradables. Dichas películas deben de tener características químicas y mecánicas apropiadas para que retarden la transferencia de masa en los sistemas de alimentos, prolonguen la vida del alimento empacado y mejoren la calidad del producto. Las películas elaboradas con aislado proteínico de suero representan una alternativa viable. Por ello el objetivo de este estudio es determinar la mejor formulación para dichas películas, a partir de los componentes básicos aislado de proteínico de suero (WPI, sorbitol no cristalizable, cera de abeja, cera de candelilla, extracto libre de células (ELC además de la medición de propiedades mecánicas de diferentes combinaciones y concentraciones de los ingredientes básicos. La metodología empleada fue preparar 21 tratamientos de películas biodegradables con aislado proteínico de suero. Posteriormente se realizaron pruebas mecánicas para la determinación del porcentaje de elongación (%E, resistencia a la tensión (RT y módulo de elasticidad (ME para la determinación de la mejor formulación. Se observó que los tratamientos que tienen 10% (w/v de sorbitol (tratamientos 5, 9,14 presentaron mejores propiedades mecánicas como un %E alto y ME alto además de una RT alta, ya que el sorbitol es el que le confiere mayor flexibilidad al empaque, en contraste con aquellas que fueron tratadas con 7% (w/v de sorbitol (tratamientos 2, 8, 12, 21 el cual muestra propiedades mecánicas indeseables, ya que se tornan quebradizas además de que presentan %E bajos así como ME altos

  19. Selección de hongos aislados de bagazo de caña con actividad celulasa sobre celulosa cristalina para posibles aplicaciones industriales

    Directory of Open Access Journals (Sweden)

    Yoandy Ferrer-Marcelo

    2011-01-01

    Full Text Available Las enzimas comerciales necesarias en procesos de producción de bioetanol celulósico y enriquecimiento de forrajes, son excesivamente caras. La mayoría provienen de mutantes B-glucosidasa, con la velocidad y extensión de la hidrólisis reducida, y débil acción sobre la celulosa cristalina. A partir de bagazo, se aislaron varios hongos filamentosos con capacidad de degradación de la celulosa cristalina, para ello se diseñó un medio selectivo de celulosa agar. Del total de aislados, solo 9 microorganismos mostraron un halo definido de degradación de celulosa. Se determinó el coeficiente de degradación de celulosa cristalina a través de la medición del halo formado por las colonias, y se pre-seleccionaron 5 aislados. Posteriormente, se eligieron los tres mejores productores del complejo celulasa (6, 13 y 21 mediante una fermentación sumergida por determinación de la actividad sobre papel de filtro. Se realizó una fermentación en estado sólido sobre bagazo pretratado para aumentar la actividad celulasa. Se estudió la estabilidad relativa de los extractos enzimáticos y se realizó una caracterización parcial de los mismos. Estos presentaron poca estabilidad relativa después de 48 horas, luego de un prensado simple. El mejor rango de pH de hidrólisis estuvo entre 5,5 y 6,2 y presentaron actividad enzimática a una temperatura de 40 ºC.

  20. Period, subculture number and culture color influence on the isolated Magnaporthe oryzae sporulation at the Tocantins State Influencia de la edad, número de repicagens y coloración de lo micelio en la esporulación del aislados de Magnaporthe oryzae, causante de lo añublo del arroz en el estado de Tocantins Influência da idade, número de repicagens e coloração dos micélios na esporulação de isolados de Magnaporthe oryzae, causador da brusone em arroz no estado do Tocantins

    Directory of Open Access Journals (Sweden)

    Ildon Rodrigues Nascimento

    2011-09-01

    the relation of different culture colors in BDA medium with the conidia production. For that, three experiments were carried out in complete randomized design. In the Experiment I, the cultures with 10, 14, 18, 22, 26 e 30 days after subculture were used. In the Experiment II, the cultures with 0, 1, 2, 3 and 4 subcultures from the original culture were used. In the Experiment III, the cultures were classified as Black, Center ash with wide white edges, Ash, Center ash with narrow white edges and White. Higher sporulation of the M. oryzae was obtained on the cultures with 14 days after the subculture, in the second subculture and on the black color.

    El hongo Magnaporthe oryzae, causante de lo añublo del arroz (Bruzone, presenta una serie de limitaciones a los cultivos bajo condiciones controladas. Algunas características pueden ser variables, incluso para un mismo aislado y requieren innovaciones por los investigadores para permitir lo desarrollo de las pruebas. Este estudio tuvo como objetivo verificar, en esporas aislados M. oryzae raza IA-1 recogidas en el Proyecto Río Formoso, municipalidad de Formoso do Araguaia, la durabilidad de los cultivos almacenados en el laboratorio, la cantidad máxima de repicagens para mantener la producción satisfactoria de las conidias por las colonias y la relación de diferentes colores de las culturas en medio BDA con la producción de conidias. Con este fin, se llevó a cabo tres experimentos en el diseño completamente al azar. En el estudio I, se utilizaron las culturas con 10, 14, 18, 22, 26 y 30 días después de la repicagen. En el estudio II, se utilizaron las culturas con 0, 1, 2, 3 y 4 repicagens de la cultura original. En el estudio III, los cultivos fueron clasificados como Negro, centro Gris con bordes anchos, Gris, centro Gris con bordes Blancos estrechos y Blanco. Mayores esporulaciones de M. oryzae se obtuvieron a l

  1. Cytotoxicity study of plant Aloe vera (Linn

    Directory of Open Access Journals (Sweden)

    Atul N Chandu

    2012-01-01

    Full Text Available Background: The objective of this study has been to evaluate the in-vitro antitumor activity of Aloe vera extract of in cultured B16F10 melanoma cell line by measuring cell viability using "Trypan blue exclusion assay" method. Aim: To find out such kind of anticancer drug which is a cheap, safe, less toxic, and more potent drug compared to chemotherapy drug. Materials and Methods: In-vitro antitumor activity cell culture1, drug treatment (standard and test extract and Trypan blue exclusion assay growth and viability test 1 were used. Treatment of Aloe vera extract against B16F10 melanoma cell line, in all concentration range, showed decrease in percent cell viability, as compared to that of negative when examined by "Trypan blue exclusion assay". Results: In overall variation of test samples, Aloe vera extract showed its best activity in the concentration of 300 μg/ml, which was approximately equal to the activity of standard drug doxorubicin. Evaluation of in-vitro antitumor activity revealed that Aloe vera extract exhibits good cytotoxic activity. The best cytotoxic activity by Aloe vera was shown at 200 μg/ml concentration. Conclusion: The study of cytoprotection against normal cells by micronucleus assay has shown that the herbal extracts have less toxic effects to the normal blood lymphocytes, as compared to that of standard anticancer drug.

  2. Gamma irradiation improves the antioxidant activity of Aloe vera (Aloe barbadensis miller) extracts

    Science.gov (United States)

    Mi Lee, Eun; Bai, Hyoung-Woo; Sik Lee, Seung; Hyun Hong, Sung; Cho, Jae-Young; Yeoup Chung, Byung

    2012-08-01

    Aloe has been widely used in food products, pharmaceuticals, and cosmetics because of its aromatic and therapeutic properties. In the present study, the ethanolic extracts of aloe gel were gamma-irradiated from 10 to 100 kGy. After gamma irradiation, the color of the ethanolic extracts of aloe gel changed to red; this color persisted up to 40 kGy but disappeared above 50 kGy. Liquid chromatography/mass spectrometry analysis demonstrated the production of a new, unknown compound (m/z=132) after gamma irradiation of the ethanolic extracts of aloe gel. The amount of this unknown compound increased with increasing irradiation up to 80 kGy, and it was degraded at 100 kGy. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by the 1,1-diphenyl-2-picrylhydrazyl-radical scavenging capacity. The antioxidant activity of aloe extract was dramatically increased from 53.9% in the non-irradiated sample to 92.8% in the sample irradiated at 40 kGy. This strong antioxidant activity was retained even at 100 kGy. These results indicate that gamma irradiation of aloe extract can enhance its antioxidant activity through the formation of a new compound. Based on these results, increased antioxidant activity of aloe extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  3. Obtención y aplicaciones de concentrados y aislados protéicos

    Directory of Open Access Journals (Sweden)

    Millán, Francisco

    2001-04-01

    Full Text Available A review on the production of protein concentrates and isolates and their use in human foods has been carried out. The three methods usually used in the obtent ion of protein concentrates are described: extraction with water, with thermic treatment or with hydroalcoholic solutions. Also the most common methods used for the obtention of protein isolates are described, including isoelectric precipitation or protein recovery by ultrafiltration. Applications of protein isolates in human foods, such as nutritionals or functionals, are also described.Se ha real izado una revisión sobre la obtención de concentrados y aislados proteicos vegetales y sus aplicaciones en alimentación humana. Se describen los tres métodos más comunes para la obtención de concentrados proteicos: extracción con agua, con tratamiento térmico o con soluciones hidroalcoholicas. También se describe los métodos más frecuentes de obtención de aislados proteicos, que incluyen en su segunda fase la precipitación isoelectrica de las proteínas o la recuperación mediante ultrafiltración de las mismas. Por último se citan las aplicaciones más importantes de los aislados en alimentación humana, como pueden ser nutricionales o funcionales.

  4. Revisión de la aloe vera (Barbadensis Miller en la dermatología actual Revision Of Aloe Vera (Barbadensis Miller In Actual Dermatology

    Directory of Open Access Journals (Sweden)

    GM Ferraro

    2009-12-01

    Full Text Available El Aloe vera es una planta de uso popular en el tratamiento de algunas enfermedades de la piel y de aplicación frecuente en la cosmetología. Forma parte de diversas cremas o geles, siendo usado como antiinflamatorio y reconstituyente del tejido epitelial. Aunque se conocen varios mecanismos de acción e indicaciones clínicas, se debe realizar una revisión de las mismas para su uso, avalados por ensayos controlados para determinar su eficacia real.The Aloe vera is a popular plant used in the treatment of certain skin diseases that has a frequent application in cosmetology. It forms a part of diverse creams or gels, being used as anti-inflammatory and restorative of epithelial tissue. Although there are several mechanisms of action and clinical indications, a review of its use should be done supported by controlled trials to determine its effectiveness.

  5. Trolox reduces the effect of ethanol on acetylcholine-induced contractions and oxidative stress in the isolated rabbit duodenum El Trolox reduce el efecto del etanol sobre las contracciones inducidas a la acetilcolina y el estrés oxidativo en duodeno aislado de conejo

    Directory of Open Access Journals (Sweden)

    Diego S. Fagundes

    2011-08-01

    Full Text Available Trolox is a hydrophilic analogue of vitamin E and a free radical scavenger. Ethanol diminishes the amplitude of spontaneous contractions and acetylcholine (ACh-induced contractions in rabbit duodenum. The aim of this work was to study the effect of Trolox on the alterations induced by ethanol on contractility and lipid peroxidation in the duodenum. The duodenal contractility studies in vitro were carried out in an organ bath and the levels of malondialdehyde and 4-hydroxyalkenals (MDA+4-HAD were measured by spectrophotometry. Trolox increased the reduction induced by ethanol on the amplitude of spontaneous contractions in longitudinal muscle but not in circular muscle. Trolox 4 mM decreased the effects of ethanol on ACh-induced contractions and on MDA+4-HDA concentrations. We conclude that Trolox might prevent oxidative stress induced by ethanol in the duodenum.El Trolox es un análogo hidrofílico de la vitamina E y un agente que secuestra radicales libres. El etanol disminuye la amplitud de las contracciones espontáneas y las contracciones inducidas a la acetilcolina en el duodeno de conejo. El objetivo de este trabajo era estudiar el efecto del Trolox en las alteraciones inducidas por el etanol sobre la contractilidad y la peroxidación lipídica en el duodeno. Los estudios de contractilidad duodenal in vitro se realizaron en un baño de órganos y los niveles de MDA+4-HDA se midieron por espectofotometría. El Trolox aumentó la reducción inducida por el etanol sobre la amplitud de las contracciones espontáneas en el músculo longitudinal pero no en el músculo circular de duodeno. El Trolox 4 mM redujo los efectos del etanol sobre las contracciones inducidas a la acetilcolina y sobre las concentraciones de MDA+4-HDA. Se concluye que el Trolox podría prevenir el estrés oxidativo inducido por el etanol en el duodeno.

  6. Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

    Science.gov (United States)

    Cristiano, Giuseppe; Murillo-Amador, Bernardo; De Lucia, Barbara

    2016-01-01

    Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems. PMID:27721816

  7. PROPAGATION TECHNIQUES AND AGRONOMIC REQUIREMENTS FOR THE CULTIVATION OF BARBADOS ALOE (ALOE VERA (L. BURM. F. - A REVIEW.

    Directory of Open Access Journals (Sweden)

    Barbara De Lucia

    2016-09-01

    Full Text Available Barbados aloe (Aloe vera (L. Burm. f. has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe and America.In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems.

  8. Algunos componentes esteroidales del solanum havanense jacq

    Directory of Open Access Journals (Sweden)

    Mario J. Basterrechea Rey

    2000-01-01

    Full Text Available Un nuevo alcaloide esteroidal llamado acetil etiolina, ha sido aislado de las hojas, tallos y raices del Solanum havanense Jacq y su estructura corresponde con (25S-16a-acetoxi-22.26-epiminocolest-5.22(N-dien-3ß-ol.

  9. The effects of Aloe vera (Aloe barbadensis) coating on the quality of shrimp during cold storage.

    Science.gov (United States)

    Soltanizadeh, Nafiseh; Mousavinejad, Mohsen S

    2015-10-01

    Green tiger shrimp (Penaeus semisulcatus) is an important aquaculture species worldwide. Its perishable nature, however, needs preservation methods to ensure its quality and shelf life. In this study, the effects of Aloe vera coating on the quality and shelf life of shrimps during cold storage were investigated. Shrimp samples were dipped in aqueous solutions containing 25%, 50%, 75%, and 100% Aloe vera gel before storage at 4 °C for 7 days. Drip loss, pH, TBA, TVB-N, and texture of both the control and treated shrimp samples were analyzed periodically. There were significant differences between coated shrimps and the control group in all parameters evaluated. Aloe vera at 75% and 100% concentrations was able to prevent lipid oxidation and drip loss properly; however, coatings containing 25% Aloe vera did not have the desired effects on these characteristics. Shrimps coated with higher concentrations of Aloe vera had better textural properties during cold storage. Results also indicated the positive effects of Aloe vera coating on the sensory quality of shrimp.

  10. Resistencia y susceptibilidad de microorganismos aislados en pacientes atendidos en una institución hospitalaria de tercer nivel, Villavicencio-Colombia, 2012

    Directory of Open Access Journals (Sweden)

    Oscar Alexander Gutiérrez Lesmes

    2015-05-01

    Full Text Available Introducción: La resistencia bacteriana es problema significativo de salud pública y está asociada al uso indiscriminado e irracional de antibióticos, lo que afecta la eficacia de los tratamientos. Objetivo: Estimar la resistencia antimicrobiana a los antibióticos resultado de las pruebas invitro de susceptibilidad en una institución hospitalaria de tercer nivel de Villavicencio, Colombia. Materiales y Métodos: Se realizó un estudio observacional, descriptivo de tipo retrospectivo, análisis univariado, calculando medias de las variables susceptibilidad, resistencia, antibiótico, microorganismos, y tipo de muestra resultados de las pruebas de susceptibilidad realizadas mediante técnica de Kirby-Bauer de 485 pruebas de susceptibilidad de la institución hospitalaria. Resultados y Discusión: Los microorganismos más frecuentemente aislados, fueron: Escherichia coli, Klebsiella pneumoniae ss. Pneumoniae, Staphylococcus aureus ss. Aureus representando el 49% de total de microorganismos, los mayores reportes de resistencia en la pruebas invitro las presentaron Serratia marcescens, Staphylococcus saprophyticus ss. Saprophytic, Enterobacter cloacae, presentan una resistencia media superior al 50%, y una susceptibilidad inferior 40%, la mayor resistencia media se presentó para los siguientes antibióticos, Cefalotina 72,2%, Cefazolina 59,9%, Nitrofurantoina 54,4%, Ceftriaxona 52,7%. Conclusiones: Antibióticos como la Cefalotina, Nitrofurantoina, Cefazolina y Ceftriaxona  pierden su utilidad terapéutica dada la elevada resistencia demostrada por los microorganismos aislados en las pruebas invitro, es necesario reforzar las medidas de uso adecuado de antibióticos para disminuir la posibilidad de adaptación y resistencia a los mismos,  el fenómeno de resistencia bacteriana ocurre fuera del ámbito hospitalario evidenciando la necesidad de iniciar también control y vigilancia en infecciones ocurridas en la comunidad.Cómo citar este

  11. Obtención y caracterización de aislados proteicos de colza

    Directory of Open Access Journals (Sweden)

    Gonçalves, N.

    1997-10-01

    Full Text Available A method for the obtention of protein isolates from defatted rapeseed flour has been designed. The process includes a basic extraction followed by a precipitation at the isoelectric point of the proteins. The precipitate is washed with water (pH 4.5, ethanol and acetone, obtaining a protein isolate with 86% of protein and reducing the contents in polyphenols and soluble sugars in more than 90% with respect to the defatted flour. The final product have physico-chemical characteristics that make it atractive to be used as food and for the obtention of protein hydrolizates.

    Se ha diseñado un proceso de obtención de aislados proteicos a partir de harina de colza desengrasada. El método incluye la extracción básica de las proteínas solubles seguido de una precipitación acida en el punto isoeléctrico. El precipitado es lavado con agua (pH 4.5, etanol y acetona, obteniéndose un aislado proteico con un 86% de proteína y reduciéndose el contenido en polifenoles y azúcares solubles en más de un 90% respecto a la harina desengrasada. El aislado final presenta unas características físico-químicas que lo hacen atractivo para su uso en alimentación y obtención de hidrolizados proteicos.

  12. Biodiversidad de Bacillus thuringiensis aislados de agroecosistemas peruanos y evaluación del potencial bioinsecticida

    OpenAIRE

    Flores P, Abad; Laboratorio de Microbiología Ambiental y Biotecnología, Facultad de Ciencias Biológicas.; Egúsquiza Ch., Rosa m.; Laboratorio de Microbiología Ambiental y Biotecnología, Facultad de Ciencias Biológicas.; Alcarraz C., Mario; Laboratorio de Bioprocesos Industriales, Facultad de Ciencias Biológicas.; Woolcott H., Juan C.; Laboratorio de Microbiología Ambiental y Biotecnología, Facultad de Ciencias Biológicas.; Benavides R, Elena; Laboratorio de Bioquímica y Biotecnología, Facultad de Farmacia y Bioquímica. Universidad Nacional Mayor de San Marcos, Lima, Perú.; Godoy A., Juan; Laboratorio de Bioquímica y Biotecnología, Facultad de Farmacia y Bioquímica. Universidad Nacional Mayor de San Marcos, Lima, Perú.; Huerta C., Doris; Laboratorio de Microbiología Ambiental y Biotecnología, Facultad de Ciencias Biológicas.; Jesus M., Yisella; Laboratorio de Microbiología Ambiental y Biotecnología, Facultad de Ciencias Biológicas.; Patiño G., Alejandro; Laboratorio de Microbiología Ambiental y Biotecnología, Facultad de Ciencias Biológicas.

    2014-01-01

    El control de plagas en la agricultura peruana se ha caracterizado por el uso intensivo de plaguicidas químicos; sin embargo, las pérdidas en el rendimiento y la aparición de resistencia en las plagas han llevado a los productores a incrementar el uso de plaguicidas. Este uso indiscriminado genera problemas de contaminación ambiental, muerte de la fauna benéfica, presencia de residuos químicos en los frutos, riesgo de intoxicaciones agudas y crónicas; además, incremento en los costos de produ...

  13. Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera

    DEFF Research Database (Denmark)

    Grace, Olwen Megan; Buerki, Sven; Symonds, Matthew RE

    2015-01-01

    the rich traditions of medicinal use and leaf succulence in aloes. Results: The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major...

  14. Topical Aloe Vera (Aloe barbadensis Miller) Extract Does Not Accelerate the Oral Wound Healing in Rats.

    Science.gov (United States)

    Coelho, Fernanda Hack; Salvadori, Gabriela; Rados, Pantelis Varvaki; Magnusson, Alessandra; Danilevicz, Chris Krebs; Meurer, Luise; Martins, Manoela Domingues

    2015-07-01

    The effect of topical application of Aloe Vera (Aloe barbadensis Miller) extract was assessed on the healing of rat oral wounds in an in vivo model using 72 male Wistar rats divided into three groups (n = 24): control, placebo and Aloe Vera (0.5% extract hydroalcoholic). Traumatic ulcers were caused in the dorsum of the tongue using a 3-mm punch tool. The Aloe Vera and placebo group received two daily applications. The animals were sacrificed after 1, 5, 10 and 14 days. Clinical analysis (ulcer area and percentage of repair) and histopathological analysis (degree of re-epithelialization and inflammation) were performed. The comparison of the differences between scores based on group and experimental period, both in quantitative and semi-quantitative analyses, was performed using the Kruskal-Wallis test. The significance level was 5%. On day 1, all groups showed predominantly acute inflammatory infiltrate. On day 5, there was partial epithelialization and chronic inflammatory infiltrate. On the days 10 and 14 total repair of ulcers was observed. There was no significant difference between groups in the repair of mouth ulcers. It is concluded that treatment using Aloe Vera as an herbal formulation did not accelerate oral wound healing in rats.

  15. Phenolic constituents in dried flowers of aloe vera (Aloe barbadensis) and their in vitro antioxidative capacity.

    Science.gov (United States)

    Keyhanian, Shirin; Stahl-Biskup, Elisabeth

    2007-06-01

    The dried flowers from Aloe vera (L.) Burm. f. (Aloe barbadensis Mill.) (Asphodelaceae) were analysed by means of HPLC-DAD and HPLC-MS/MS, verifying chlorogenic, caffeic, 5-P-coumaroylquinic, caffeoylshikimic, 5-feruloylquinic, 5-P-CIS-coumaroylquinic, P-coumaric and ferulic acid as well as luteolin, apigenin, quercetin, kaempferol, isoorientin, isovitexin and their 7-O-glucosides, saponarin and lutonarin. On searching for anthranoids in the flower extract, aloe-emodin as well as the glycosylchromone aloeresin B could be identified. Aloin A and B, the laxative principle of the drug Curaçao-Aloes, are not accumulated in the dried flowers. The polyphenol content of three different batches was 0.73 - 1.01% (+/- 0.05%) and the flavonoid content 0.24 - 0.34% (+/- 0.01%). The hydrophilic antioxidative capacity amounted to 85.7 - 94.9 (+/- 0.5) micromol TEAC/g dried Aloe vera flower and was directly correlated with the polyphenol and flavonoid contents.

  16. Allionrs Aloe names (Asphodelaceae: nomenclature and typification

    Directory of Open Access Journals (Sweden)

    L. Gugliemone

    2009-12-01

    Full Text Available The taxa belonging to the genus Aloe published in Synopsis methodica stirpium horti regii taurinensis (Allioni 1760 and in Auctarium ad synopsim meihodicam stirpium horti regii taurinensis (Allioni 1773 were examined. The protologues of Aloe maculata All. and A. verrucosospinosa All. are analysed and lectotypes designated. The homonymy of A. succotrina All. w ith A. succotrina Weston (1770 is recognized, and the lectotype o f this last name designated. Epitypes are selected to fix the application of all three names. Short differential diagnoses o f the three species are given and their distribution ranges discussed; distribution maps based on specimens held in the South African National Herbarium (PRE, KwaZulu-Natal Herbarium (NH. Compton Herbarium (NBG and the South African Museum Collection (SAM held in NBG are provided.

  17. Sensitivity of Venturia inaequalis Chilean Isolates to Difenoconazole, Fenarimol, Mancozeb, and Pyrimethanil Sensibilidad de Aislados Chilenos de Venturia inaequalis a Difenoconazole, Fenarimol, Mancozeb y Pyrimethanil

    Directory of Open Access Journals (Sweden)

    Jose Luis Henríquez S

    2011-03-01

    Full Text Available Apple scab (Venturia inaequalis is the most important disease in Chilean apple (Malus domestica Borkh. orchards where fungicide management is the main tool to control disease. Sensitivity tests to difenoconazole, fenarimol, mancozeb, and pyrimethanil fungicides were conducted on V. inaequalis wild isolates to construct local baseline sensitivity distributions, and compare them with a collection of isolates from seven different commercial orchards with no evidence of practical resistance. In vitro assays were conducted and sensitivity was measured as inhibition of mycelial growth (difenoconazole, fenarimol, pyrimethanil or conidial germination (mancozeb. Departure from the baseline distribution to less sensitivity was found for difenoconazole, fenarimol, and mancozeb with resistance factors of 4.7, 5.8, and 2.1, respectively. The highest difference between baseline and orchard populations was observed for fenarimol which showed the highest shift to less sensitivity. Discriminatory doses of 0.04, 1.0, 0.6, and 0.2 µg mL-1 are proposed for in vitro monitoring of sensitivity to difenoconazole, fenarimol, mancozeb, and pyrimethanil, respectively.La sarna del manzano (Venturia inaequalis es la principal enfermedad del cultivo en Chile y es controlada principalmente con el uso de fungicidas. Se realizaron pruebas de sensibilidad a los fungicidas difenoconazole, fenarimol, mancozeb y pyrimethanil en aislados monoconidiales de cepas silvestres de V. inaequalis con el fin de elaborar curvas basales de sensibilidad y compararlas con la sensibilidad de una colección de aislados monoconidiales provenientes de siete huertos comerciales sin evidencias de resistencia práctica. Se realizaron pruebas in vitro donde la sensibilidad se midió como la inhibición del crecimiento miceliar (difenoconazole, fenarimol y pyrimethanil o la inhibición de la germinación de conidias (mancozeb. La población de huertos comerciales estudiada presentó una menor

  18. In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel.

    Science.gov (United States)

    Kang, Min-Cheol; Kim, Seo Young; Kim, Yoon Taek; Kim, Eun-A; Lee, Seung-Hong; Ko, Seok-Chun; Wijesinghe, W A J P; Samarakoon, Kalpa W; Kim, Young-Sun; Cho, Jin Hun; Jang, Hyeang-Su; Jeon, You-Jin

    2014-01-01

    The in vitro and in vivo antioxidant potentials of a polysaccharide isolated from aloe vera gel were investigated. Enzymatic extracts were prepared from aloe vera gel by using ten digestive enzymes including five carbohydrases and five proteases. Among them, the highest yield was obtained with the Viscozyme extract and the same extract showed the best radical scavenging activity. An active polysaccharide was purified from the Viscozyme extract using ethanol-added separation and anion exchange chromatography. Purified aloe vera polysaccharide (APS) strongly scavenged radicals including DPPH, hydroxyl and alkyl radicals. In addition, APS showed a protective effect against AAPH-induced oxidative stress and cell death in Vero cells as well as in the in vivo zebrafish model. In this study, it is proved that both the in vitro and in vivo antioxidant potentials of APS could be further utilized in relevant industrial applications.

  19. Caracterización morfoanatómica comparativa entre Aloe vera (L. Burm. F., Aloe arborescens Mill., Aloe saponaria Haw. y Aloe ciliaris Haw. (Aloeaceae

    Directory of Open Access Journals (Sweden)

    Stella Maris Carpano

    2009-03-01

    Full Text Available Aloe vera (L. Burm. F. (= Aloe barbadensis Miller conhecida como "a planta da imortalidade" no antigo Egito é utilizada em fitoterapia como humectante, antibacteriana, antifúngica, antiviral e antioxidante. Com a finalidade de contribuir para identificação da droga vegetal, foram realizados estudos morfoanatômicos da folha. A utilização de plantas com características terapêuticas reconhecidas determina que, por analogia, popularmente sejam utilizadas com a mesma finalidade outras espécies do mesmo gênero. Isto é o que ocorre com três espécies de Aloe spp., que se cultivam ou crescem acidentalmente na Argentina: A. arborescens Mill., A. saponaria Haw. e A. ciliaris Haw. (Aloaceae. Estabeleceram-se os caracteres morfoanatômicos de diagnóstico por microscopia óptica e microscopia eletrônica de varredura (apresentam-se desenhos e fotomicrografias dos mesmos. Obtiveram-se valores numéricos dos elementos histológicos de diagnóstico: magnitudes lineares e proporcionais. Realizaram-se reações histoquímicas de identificação dos princípios ativos para sua localização "in situ".

  20. Aloe Vera and Infected Leg Ulcers

    Directory of Open Access Journals (Sweden)

    Asima Banu

    2012-06-01

    Full Text Available Background Infected leg ulcers are major health problems resulting in morbidity and disability and are usually chronic and refractory to antimicrobial treatment. Aims The present study is aimed at determining the bacteria involved in leg ulcers and their resistance patterns to commonly used antibiotics as well as to determine whether Aloe Vera has antibacterial activity against multi-drug resistant organisms and promotes wound healing. Method A total of 30 cases with leg ulcers infected with multi-drug resistant organisms were treated with topical aloe vera gel and 30 age and sex-matched controls were treated with topical antibiotics. Culture and sensitivity was done from the wounds on alternate days and the ulcer was clinically and microbiologically assessed after 10 days. The results were compiled and statistically analysed. Results Cultures of the study group who were using aloe vera dressings showed no growth by the fifth day in 10 (33.3% cases, seventh day in another 16 (53.3% and ninth day in two of the remaining four cases (6.7% while in two (6.7% cases there was no decrease in the bacterial count. This means that of the 30 cases, 28 showed no growth by the end of 11 days while two cases showed no decrease in bacterial count. Growth of bacteria in study group is decreased from 100% (30 cases to 6.7% (2 cases by day 11 with P

  1. Selective Solid-phase Extraction of Aloe Emodin from Aloe by Molecularly Imprinted Polymers

    Institute of Scientific and Technical Information of China (English)

    TIAN Ming-lei; LEE Yu-ri; PARK Dong-wha; ROW Kyung-ho

    2013-01-01

    The extraction and separation of aloe emodin were optimized via selective molecularly imprinted solid-phase extraction.Molecularly imprinted polymer was prepared from the functional monomer,methacrylic acid and a mixture of ethanol/dodecanol(90/10,volume ratio) as porogen.It overcomes the common problems of imprinting biological polar compounds and shows high selectivity compared favorably with those of non-imprinted polymer and commercially available C18 and silica cartridges in similar aloe emodin tests.Good linearity was obtained between 0.002 and 2.5 mg/mL(r2=0.998) with relative standard deviations below 3.3%.

  2. Caracterización de hongos aislados de mapas conservados en el Archivo Nacional de la República de Cuba

    Directory of Open Access Journals (Sweden)

    Alian Molina Veloso

    2014-12-01

    Full Text Available Los microorganismos, principalmente los hongos filamentosos (mohos, generan problemas en archivos, bibliotecas y museos provocando el biodeterioro de las colecciones. Estos efectos son más acusados en países de clima tropical debido a la influenciade las altas temperaturas y humedad relativa. Los objetivos de este estudio fueron caracterizar la concentración fúngica en cinco mapas conservados en la Mapoteca del Archivo Nacional de Cuba y determinar el potencial biodeteriorante de los hongos aislados. La toma de muestra se realizó mediante la técnica del hisopado en forma aséptica. Se determinó cualitativamente la actividad celulolítica, amilolítica, proteolítica así como la excreción de pigmentos y de ácidos orgánicos. Se detectó una concentración fúngica media de 622 ufc.cm-2, además de especies que constituyeron nuevos hallazgos en soportes documentales para el Archivo Nacional de Cuba. Los hongos degradaron la celulosa, el almidón y la gelatina además de excretar ácidos orgánicos y pigmentos.

  3. O uso do Aloe sp (aloe vera em feridas agudas e crônicas: revisão integrativa

    Directory of Open Access Journals (Sweden)

    Lucélia Terra Chini

    2017-01-01

    Full Text Available Objetivos: buscar evidencias disponibles en la literatura acerca del uso de Aloe sp (sábila en la cicatrización de heridas agudas y crónicas. Materiales y método: se trata de una revisión integradora realizada en las bases de datos LILACS, PubMed y Scopus, en el periodo de febrero a marzo del 2015. La búsqueda resultó en 178 publicaciones. Resultados: siete estudios constituyeron la revisión, los cuales involucraron personas con heridas de cesárea, heridas de episiotomía, quemaduras, área donadora de injerto, heridas posthemorroidectomía y heridas fisuarias anales crónicas. Se evidenció que la sábila promueve la cicatrización de heridas, además de disminuir el dolor en fisuras anales crónicas y quemaduras. Conclusiones: la sábila representa una nueva terapéutica en el tratamiento de heridas; sin embargo, las evidencias disponibles sobre su eficacia y seguridad son ineficientes para legitimar su uso en la cicatrización de heridas agudas y crónicas; por lo tanto, no se pueden generalizar.

  4. La contaminación microbiana del aire en dos depósitos del Archivo Nacional de la República de Cuba

    OpenAIRE

    Sofía Borrego; Vanessa Pons; Ivette Perdomo

    2008-01-01

    Los objetivos del estudio fueron determinar la concentración microbiana en el aire de dos depósitos del Archivo Nacional de Cuba, realizar la caracterización fisiológica de hongos aislados y describir brevemente las características patogénicas de los microorganismos aislados. Para el aislamiento se emplearon placas de Petri expuestas según la metodología de Omeliansky. La actividad celulolítica, la producción de pigmentos y de ácidos en hongos, se determinó empleando un medio de cultivo salin...

  5. Aloe vera as a functional ingredient in foods.

    Science.gov (United States)

    Rodríguez Rodríguez, Elena; Darias Martín, Jacinto; Díaz Romero, Carlos

    2010-04-01

    The main scientific discoveries on Aloe vera published mainly in the last three decades are presented in this work. After describing Aloe from a botanical point of view, the papers related with the chemical composition of different parts of the leaf of Aloe, particularly those in which the gel is described and are presented in a synthetic manner. The chemical analyses reveal that Aloe gel contains mannose polymers with some glucose and other sugars, among which the most important is Acemannan. Besides these, other components such as glycoproteins, enzymes, amino acids, vitamins, and minerals are described. Different factors also affecting the chemical composition of the gel, such as species and variety, climatic and soil conditions, cultivation methods, processing and preservation, are enumerated and discussed. On the other hand, the main therapeutic applications have been revised and the possible damaging effects of Aloe are also commented upon. A special emphasis is placed on the biologically active compounds or groups of compounds responsible for the therapeutic applications and which are their action mechanisms. The paper concludes that more research is needed to confirm the therapeutic and beneficial effects and to definitively clarify the myth surrounding Aloe vera. A general view on the problem of the commercialization and establishment of the quality and safety of Aloe products in the food industry has been offered here. The main points and European regulations that need to be considered regarding the quality control of prepared Aloe products are presented in this paper.

  6. Aloe vera: A review of toxicity and adverse clinical effects.

    Science.gov (United States)

    Guo, Xiaoqing; Mei, Nan

    2016-04-01

    The Aloe plant is employed as a dietary supplement in a variety of foods and as an ingredient in cosmetic products. The widespread human exposure and its potential toxic and carcinogenic activities raise safety concerns. Chemical analysis reveals that the Aloe plant contains various polysaccharides and phenolic chemicals, notably anthraquinones. Ingestion of Aloe preparations is associated with diarrhea, hypokalemia, pseudomelanosis coli, kidney failure, as well as phototoxicity and hypersensitive reactions. Recently, Aloe vera whole leaf extract showed clear evidence of carcinogenic activity in rats, and was classified by the International Agency for Research on Cancer as a possible human carcinogen (Group 2B). This review presents updated information on the toxicological effects, including the cytotoxicity, genotoxicity, carcinogenicity, and adverse clinical effects of Aloe vera whole leaf extract, gel, and latex.

  7. Qualitative improvement of low meat beef burger using Aloe vera.

    Science.gov (United States)

    Soltanizadeh, Nafiseh; Ghiasi-Esfahani, Hossein

    2015-01-01

    Low meat beef burgers have found their niche in the food markets in developing countries because of their lower price. However, these burgers still lack an acceptable quality. This study investigates the effects of different concentrations of Aloe vera on the quality of this food product. For this purpose, beef burgers were produced with 0%, 1%, 3%, and 5% Aloe vera and the changes in their cooking parameters, lipid oxidation, texture, and appeal to consumers over 7days of refrigerated storage were evaluated. Results indicate that Aloe vera contributed to some extent to decreased cooking loss and diameter reduction in the burgers. Increased concentrations of Aloe vera led to improvements in the water absorption and texture of the burgers as well as their lipid stability. However, a concentration level of 3% led to the most acceptability of the product to the panelists. Finally, it was found that Aloe vera acts as a hydrocolloid and improves the quality of burgers.

  8. Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera) Grown under Water Stress.

    Science.gov (United States)

    Salinas, Carlos; Handford, Michael; Pauly, Markus; Dupree, Paul; Cardemil, Liliana

    2016-01-01

    Aloe barbadensis Miller (Aloe vera) has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among the molecules responsible for tolerating water deficit in other plant species. Nevertheless, fructans, which are prebiotic compounds, have not been described nor studied in Aloe vera, whose leaf gel is known to possess beneficial pharmaceutical, nutritional and cosmetic properties. As Aloe vera is frequently cultivated in semi-arid conditions, like those found in northern Chile, we investigated the effect of water deficit on fructan composition and structure. For this, plants were subjected to different irrigation regimes of 100%, 75%, 50% and 25% field capacity (FC). There was a significant increase in the total sugars, soluble sugars and oligo and polyfructans in plants subjected to water deficit, compared to the control condition (100% FC) in both leaf tips and bases. The amounts of fructans were also greater in the bases compared to the leaf tips in all water treatments. Fructans also increase in degree of polymerization with increasing water deficit. Glycosidic linkage analyses by GC-MS, led to the conclusion that there are structural differences between the fructans present in the leaves of control plants with respect to plants irrigated with 50% and 25% FC. Therefore, in non-stressed plants, the inulin, neo-inulin and neo-levan type of fructans predominate, while in the most stressful conditions for the plant, Aloe vera also synthesizes fructans with a more branched structure, the neofructans. To our knowledge, the synthesis and the protective role of neo-fructans under extreme water deficit has not been previously reported.

  9. Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera Grown under Water Stress.

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    Carlos Salinas

    Full Text Available Aloe barbadensis Miller (Aloe vera has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among the molecules responsible for tolerating water deficit in other plant species. Nevertheless, fructans, which are prebiotic compounds, have not been described nor studied in Aloe vera, whose leaf gel is known to possess beneficial pharmaceutical, nutritional and cosmetic properties. As Aloe vera is frequently cultivated in semi-arid conditions, like those found in northern Chile, we investigated the effect of water deficit on fructan composition and structure. For this, plants were subjected to different irrigation regimes of 100%, 75%, 50% and 25% field capacity (FC. There was a significant increase in the total sugars, soluble sugars and oligo and polyfructans in plants subjected to water deficit, compared to the control condition (100% FC in both leaf tips and bases. The amounts of fructans were also greater in the bases compared to the leaf tips in all water treatments. Fructans also increase in degree of polymerization with increasing water deficit. Glycosidic linkage analyses by GC-MS, led to the conclusion that there are structural differences between the fructans present in the leaves of control plants with respect to plants irrigated with 50% and 25% FC. Therefore, in non-stressed plants, the inulin, neo-inulin and neo-levan type of fructans predominate, while in the most stressful conditions for the plant, Aloe vera also synthesizes fructans with a more branched structure, the neofructans. To our knowledge, the synthesis and the protective role of neo-fructans under extreme water deficit has not been previously reported.

  10. Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera) Grown under Water Stress

    Science.gov (United States)

    Salinas, Carlos; Cardemil, Liliana

    2016-01-01

    Aloe barbadensis Miller (Aloe vera) has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among the molecules responsible for tolerating water deficit in other plant species. Nevertheless, fructans, which are prebiotic compounds, have not been described nor studied in Aloe vera, whose leaf gel is known to possess beneficial pharmaceutical, nutritional and cosmetic properties. As Aloe vera is frequently cultivated in semi-arid conditions, like those found in northern Chile, we investigated the effect of water deficit on fructan composition and structure. For this, plants were subjected to different irrigation regimes of 100%, 75%, 50% and 25% field capacity (FC). There was a significant increase in the total sugars, soluble sugars and oligo and polyfructans in plants subjected to water deficit, compared to the control condition (100% FC) in both leaf tips and bases. The amounts of fructans were also greater in the bases compared to the leaf tips in all water treatments. Fructans also increase in degree of polymerization with increasing water deficit. Glycosidic linkage analyses by GC-MS, led to the conclusion that there are structural differences between the fructans present in the leaves of control plants with respect to plants irrigated with 50% and 25% FC. Therefore, in non-stressed plants, the inulin, neo-inulin and neo-levan type of fructans predominate, while in the most stressful conditions for the plant, Aloe vera also synthesizes fructans with a more branched structure, the neofructans. To our knowledge, the synthesis and the protective role of neo-fructans under extreme water deficit has not been previously reported. PMID:27454873

  11. Absence of contact sensitization to Aloe vera (L.) Burm. f.

    Science.gov (United States)

    Reider, N; Issa, A; Hawranek, T; Schuster, C; Aberer, W; Kofler, H; Fritsch, P; Hausen, B M

    2005-12-01

    Aloe vera has been used as a cosmetic and medical remedy since ancient times and has gained increasing popularity in recent years. Despite its widespread use, reports of allergic reactions are rare. We patch tested 702 consecutive patients with an oily extract from the leaves, Aloe pulvis from the entire plant and concentrated Aloe vera gel. A specially designed questionnaire was used for the use of Aloe vera, reasons and location of application, adverse reactions, occupation, hobbies and atopy. None of the subjects showed any reaction to one of the preparations. 2 components of the plant have to be distinguished: the bark of the leaves contains anthrachinones with pro-peristaltic and potential antibiotic and anticancer properties. Constraints have been imposed due to their considerable toxic potential. Today, mostly the Aloe gel from the center of the leaves is processed. It almost exclusively consists of carbohydrates to which also many medical effects have been attributed. Carbohydrates are not likely to induce contact sensitization, which might explain the outcome of our study. However, this does not justify unrestrained promotion of Aloe products, as scientific studies investigating the claims on its constitutional effects are few in number, and the majority of them have been unable to diminish the intuitive scepticism against miracle cures, like Aloe seems to be.

  12. Degradación de tiocianato por hongos aislados de ambientes mineros y evaluación de su capacidad degradativa

    Directory of Open Access Journals (Sweden)

    Susan Medina

    2012-10-01

    Full Text Available El Perú es uno de los principales países en la extracción de minerales como el oro, zinc, plomo y estaño. En la producción de oro se utiliza cianuro, el cual es tóxico para el medio ambiente, y que por reacción natural con el azufre se convierte en tiocianato (-SCN. En el presente trabajo se aislan hongos con capacidad de degradar tiocianato procedentes de aguas y suelos de zonas mineras de Junín y Tumbes. Estas cepas se sometieron a ensayos con concentraciones de tiocianato entre 5 y 600 mM. Asimismo, se evaluó la cinética de degradación de tiocianato en medio Kwon partiendo de 1,2 g.L-1 de KSCN y luego se realizó la identificación morfológica. De un total de 58 mohos aislados, obtuvimos 4 mohos con excelentes capacidades de degradar tiocianato y que pueden ser utilizados en biorremediación. La capacidad degradativa de estas cepas fue en promedio 10,05 mg.L-1 de -SCN con una velocidad de 28,77 mg.L-1.h-1. Dos cepas fueron identificadas fenotípica y molecularmente como Fusarium trincictum usando el marcador ITS del gen rDNA.

  13. Estudios hematológicos y patológicos comparativos de cerdos inoculados con un aislado de campo y el serotipo 5 ATCC de Actinobacillus pleuropneumoniae Comparative hematological and pathological study of inoculated pigs with a field isolate and an ATCC serotype 5 of Actinobacillus pleuropneumoniae

    Directory of Open Access Journals (Sweden)

    D Muñoz

    2010-01-01

    Full Text Available Se realizó una inoculación experimental de A. pleuropneumoniae utilizando un aislado de campo y una cepa de referencia ATCC serotipo 5, para lo cual se utilizaron tres grupos de animales (n = 15 para cada grupo. El grupo 1 (G1 fue inoculado con medio estéril, el grupo (G2 con serotipo 5 ATCC y el grupo 3 (G3 fue inoculado con un aislado de campo (418/07. Los resultados mostraron diferencias significativas (P ≤ 0,05 en el recuento de leucocitos totales entre el grupo G1 v/s G2 y G1 v/s G3 y los grados de las lesiones pulmonares totales evidenciaron diferencias estadísticamente significativas (P ≤ 0,05 entre los tres grupos de estudio. Las lesiones histopatológicas pulmonares mostraron diferencias estadísticas relevantes sólo entre G1 y G3 (P ≤ 0,05. En este trabajo se verifican diferencias importantes del comportamiento entre el aislado de campo y el serotipo 5 ATCC, sobre los cambios hematológicos y las lesiones macroscópicas e histopatológicas ocasionadas por ellos, lo cual podría indicar una mayor virulencia y patogenicidad del aislado nacional. Se espera en un futuro próximo serotipificar este aislado nacional de App.An experimental inoculation of Actinobacillus pleuropneumoniae (App was carried out with a field isolate and an ATCC serotype 5. Three groups of 15 pigs each were used. Group 1 (G1 was the control group inoculated with sterile media, Group 2 was inoculated with the serotype 5 ATCC, and Group 3 (G3 was inoculated with a field isolate (418/07. The results showed statistically significant differences (P ≤ 0.05 in the total leukocytes count between G1 v/s G2 and G1 v/s G3. The total macroscopic lung lesions scores were statistically different among the 3 groups (P ≤ 0.05. However, statistical difference was found only between G1 and G3 in the histopathological lung lesions (P ≤ 0.05. This work shows a clear difference in the hematological changes and the macroscopic and histopathological lesions between the

  14. Nutritional and Phytochemical Screening of Aloe Bar Badensis

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    A.O. Adesuyi and O.A. Awosanya

    2012-01-01

    Full Text Available The aim of this research Study is to analyse qualitatively and quantitatively the Aloe barbadensis for Proximate, Anti-Nutrient and Phytochemical composition. The Proximate, Anti-Nutrient composition and Phytochemical screening of Aloe barbadensis were determined. The Proximate composition involves the Moisture content, Crude protein, Crude fibre, Crude Fat, Ash content and Carbohydrate. The Anti-Nutrients involved Oxalate, Tannins and Phytate while the Phytochemicals determined were the Saponins, Phenols, Alkaloids and Flavonoids. Aloe barbadensis was found to be rich in Carbohydrate (73.07%, so it can be used as a good source of Carborhydrate. The Protein and Fat content were found to be relatively low, (4.73 and 0.27% respectively. But Aloe barbadensis can still be used as a source of Protein and Fat. Qualitatively, Tannin, Oxalate and Phytate were found in trace amount. Tannin, Phytate and Oxalate contents were 0.155 g/100 g, 0.683 g/100 g, and 0.524 g/100 g respectively. This could affect the availability of Minerals in Aloe barbadensis. It was also discovered that Phytochemicals are present in quantities of 0.232 g/100g, 5.651 g/100 g, 2.471 g/100 g and 3.246 g/100 g for Phenols, Saponins, Alkaloids and Flavonoids respectively. This is an indication of Cosmetic and medicinal Value of Aloe barbadensis. The Sample was also found to be a rich source of minerals. Sodium and Potassium content (5280 and 10670 PPM respectively indicates the tendency of Aloe barbadensis to be able to regulate or control the osmotic balance of the body fluid as well as body pH. Aloe barbadensis is also found to be rich in Phosphorus (6657 PPM, which is essential for bone formation. Lead occur in traces. Magnesium (325.8 PPM is also present, which could help to lower the blood pressure. The overall data suggest that Aloe barbadensis has some Nutritional and Medicinal Properties.

  15. Evaluación de la actividad desulfurizadora de aislados nativos de Pseudomonas spp. en presencia de hidrocarburo Desulfurization activity evaluation of native strains of Pseudomonas spp. in the presence of hydrocarbon

    Directory of Open Access Journals (Sweden)

    Alméciga-Díaz Carlos Javier

    2005-07-01

    Full Text Available El principal inconveniente en la combustión de los hidrocarburos es la conversión del azufre y el nitrógeno a sus respectivos óxidos, los cuales participan en la formación de lluvia acida y deterioran el medio ambiente e infraestructuras. La remoción de azufre a partir de compuestos órgano-azufrados mediante el uso de microorganismos ha surgido como una alternativa frente al proceso catalítico de hidrodesulfurización (HDS. En el presente trabajo se evaluó la actividad desulfurizadora de veintitrés aislados nativos de Pseudomonas spp. sobre dibenzotiofeno (DBT, usando un sistema de fermentación con igual proporción de fase acuosa y orgánica (n-hexano en presencia de oleato de etanolamina. Los aislados 02,05 y 06 conservaron su viabilidad en este medio y presentaron una remoción de azufre entre 6,0 y 9,4%, generando los metabolitos DBT-sulfona, DBT-sulfóxido, 2-hidroxibifenilo (2-HBP y sulfato presentes en la ruta metabólica 4S. Con estos aislados se evaluó la actividad desulfurizadora sobre keroseno y se observó una remoción de azufre entre 19,9 y 62,6% y una disminución del poder calorífico entre 0,45 y 5,55%. Palabras clave: dibenzotiofeno, desulfurización, Pseudomonas spp., keroseno.The main difficulty with fossil fuel combustión lies in sulphur and nitrogen becoming converted to their respective oxides, forming part of the acid rain which deteriorates the environment and infrastructure. Removing sulphur from organo-sulfur compounds by using micro-organisms has become an alternative to hydrodesulphurisation (HDS. Twenty-three Pseudomonas spp. native strains' desulphurisation activity on dibenzothiophene (DBT was evaluated by using a fermentation system having equal proportions of aqueous and organic (n-hexane phases in the presence of ethanolamine oléate. The 02, 05 and 06 strains maintained their viability in this médium, presenting 6,0% to 9,4% sulphur removal, producing DBT-sulphone, DBT-sulphoxide, 2

  16. Of hemorrhagic shock, spherical cows and Aloe vera.

    Science.gov (United States)

    Gutierrez, Guillermo; Fuller, Stephanie P

    2004-12-01

    The central question explored in this commentary is whether the beneficial effects of an Aloe vera derived drag-reducing polymer during hemorrhagic shock is due to its O2 radical scavenging properties or to changes in blood rheology.

  17. Mundos aislados: segregación urbana y desigualdad en la ciudad de México

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    GONZALO A SARAVÍ

    2008-12-01

    Full Text Available De qué nos habla la segregación urbana en México y cuáles son sus implicaciones en este contexto en particular, son las dos preguntas centrales que guían el análisis desarrollado en este artículo. El argumento que responde a estas dos preguntas se estructura en torno a la hipótesis de una "coexistencia de mundos aislados". Para sustentar dicha hipótesis, el autor analiza teórica y empíricamente la relación entre las dimensiones objetiva y simbólica de la segregación urbana. El análisis se basa en datos secundarios y en información obtenida a partir de entrevistas semi-estructuradas con jóvenes residentes en colonias populares y estigmatizadas de la Ciudad de México.What does urban segregation mean in México and what are its implications for this particular context? This article poses these two questions. Our response is based on the hypothesis of the "co-existence of isolated worlds." In order to support and develop this hypothesis, the author analyses the relationship between the objective and symbolic dimensions of urban segregation. The analysis is based on secondary data and semi-structured interviews with young people from poor and stigmatized neighborhoods in México City.

  18. Aloe spp.--plants with vertebrate-like telomeric sequences.

    Science.gov (United States)

    Weiss, Hanna; Scherthan, Harry

    2002-01-01

    Chromosome termini of most eukaryotes end in tracks of short tandemly repeated GC-rich sequences, the composition of which varies among different groups of organisms. Plant species predominantly contain (TTTAGGG)n repeats at their telomeres. However, a few plant species, including members of Alliaceae and Aloe spp. (Asphodelaceae) were found to lack such Arabidopsis-type (T3AG3)n telomeric repeats. Recently, it has been proposed that the lack of T3AG3 telomeric repeat sequences extends to all species forming the Asparagales clade. Here, we analysed the composition of Aloe telomeres by single-primer PCR and fluorescence in-situ hybridization (FISH) with directly labelled Arabidopsis-type (TTTAGGG)28-43 DNA probe, and with vertebrate-type (TTAGGG)33-50 DNA and a (C3TA2)3 peptide nucleic acid (PNA) probe. It was found that Nicotiana tabacum contained Arabidopsis-type telomeric repeats, while Aloe telomeres lacked the corresponding FISH signals. Surprisingly, FISH with the highly specific vertebrate-type (C3TA2)3 PNA probe resulted in strong T2AG3-specific FISH signals at the ends of chromosomes of both Aloe and Nicotiana tabacum, suggesting the presence of T2AG3 telomeric repeats in these species. FISH with a long (TTAGGG)33-50 DNA probe also highlighted Aloe chromosome ends, while this probe failed to reveal FISH signals on tobacco chromosomes. These results indicate the presence of vertebrate-like telomeric sequences at the telomeres of Aloe spp. chromosomes. However, single-primer PCR with (TAG3)5 primers failed to amplify such sequences in Aloe, which could indicate a low copy number of T2AG3 repeats at the chromosome ends and/or their co-orientation and interspersion with other repeat types. Our results suggest that telomeres of plant species, which were thought to lack GC-rich repeats, may in fact contain variant repeat types.

  19. Liberación de endotelina-1 por angiotensina ll en miocitos cardíacos aislados Angiotensin II-induced endothelin-1 release in cardiac myocytes

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    María C. Villa-Abrille

    2006-06-01

    Full Text Available Muchos de los efectos de la angiotensina II (Ang II son mediados en realidad por la acción de endotelina (ET endógena liberada y/o producida en respuesta a la Ang II. En este trabajo evaluamos la interacción Ang II/ET-1, sus consecuencias en la contractilidad cardíaca y el papel de las especies reactivas del oxígeno (EROs. Se usaron cardiomiocitos aislados de gato. La Ang II, 1 nM, produjo un efecto inotrópico positivo (EIP de 31.8±3.8% que fue cancelado por inhibición de los receptores AT1, de los receptores de ET, del intercambiador Na+/H+ (NHE, del modo inverso del intercambiador Na+/Ca2+ (NCX o por el secuestro de EROs. La Ang II, 100 nM, produjo un EIP de 70.5±7.6% que fue cancelado por inhibición de los receptores AT1 y bloqueado en parte por inhibición de los receptores de ET, del NHE, del modo inverso del NCX o por el secuestro de EROs. La Ang II, 1 nM, incrementó el ARNm de la preproET-1 lo cual fue anulado por el bloqueo de los receptores AT1. Los resultados permiten concluir que el EIP de la Ang II es debido a la acción de la ET-1 endógena liberada/formada por la Ang II. La ET-1 produce: estimulación del NHE, activación del modo inverso del NCX y un consecuente EIP. Dentro de esta cascada también participarían los EROs.Many of the effects thought to be due to angiotensin II (Ang II are due to the release/formation of endothelin (ET. We tested whether Ang II elicits its positive inotropic effect (PIE by the action of endogenous ET-1 and the role played by the reactive oxygen species (ROS in this mechanism. Experiments were performed in cat isolated ventricular myocytes in which sarcomere shortening (SS was measured to asses contractility after pharmacological interventions and the effect of Ang II on inotropism were analyzed. Ang II 1 nM increased SS by 31.8±3.8% (p<0.05. This PIE was cancelled by AT1 receptor blockade, by ET-1 receptors blockade, by Na+/H+ exchanger (NHE inhibition, by reverse mode Na+/Ca2

  20. Use of Random Amplified Polymorphic DNA (RAPD) Technique to Study the Genetic Diversity of Eight Aloe Species.

    Science.gov (United States)

    Ezzat, Shahira M; El Sayed, Abeer M; Salama, Maha M

    2016-10-01

    The genus Aloe comprises over 400 species of flowering succulent plants. Aloe leaves are used in the treatment of asthma, gastrointestinal ulcers, cardiovascular disease, tumors, burns, and diabetes. They are rich in anthraquinones, such as aloin, aloe-emodin, chrysophanol, aloinoside A, and aloinoside B. The various species of Aloe show chemical and morphological similarity and diversity, which depend on the genotype and environmental conditions. In a continuity to our interest in the genus Aloe, this study targets the authentication of eight different Aloe species, Aloe vera (A1), Aloe arborescens (A2), Aloe eru (A3), Aloe grandidentata (A4), Aloe perfoliata (A5), Aloe brevifolia (A6), Aloe saponaria (A7), and Aloe ferox (A8), grown in Egypt by using the technique of random amplified polymorphic DNA. Twelve decamer primers were screened in amplification with genomic DNA extracted from all species, of which five primers yielded species-specific reproducible bands. Out of 156 loci detected, the polymorphic, monomorphic, and unique loci were 107, 26, and 23, respectively. Based on a dendrogram and similarity matrix, the eight Aloe species were differentiated from each other and showed more divergence. Aloe species prevailed similarity coefficients of 54-70 % by which they could be classified into three major groups. Thus, this technique may contribute to the identification of these Aloe species that have great morphological similarity in the Egyptian local markets.

  1. Aloe vera for treating acute and chronic wounds

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    Anthony D. Dat

    Full Text Available BACKGROUND: Aloe vera is a cactus-like perennial succulent belonging to the Liliaceae Family that is commonly grown in tropical climates. Animal studies have suggested that Aloe vera may help accelerate the wound healing process.OBJECTIVE: To determine the effects of Aloe vera-derived products (for example dressings and topical gels on the healing of acute wounds (for example lacerations, surgical incisions and burns and chronic wounds (for example infected wounds, arterial and venous ulcers.METHODS:Search methods: We searched the Cochrane Wounds Group Specialised Register (9 September 2011, the Cochrane Central Register of Controlled Trials (CENTRAL (The Cochrane Library 2011, Issue 3, Ovid MEDLINE (2005 to August Week 5 2011, Ovid MEDLINE (In-Process & Other Non-Indexed Citations 8 September 2011, Ovid EMBASE (2007 to 2010 Week 35, Ovid AMED (1985 to September 2011 and EBSCO CINAHL (1982 to 9 September 2011. We did not apply date or language restrictions. Selection criteria: We included all randomised controlled trials that evaluated the effectiveness of Aloe vera, aloe-derived products and a combination of Aloe vera and other dressings as a treatment for acute or chronic wounds. There was no restriction in terms of source, date of publication or language. An objective measure of wound healing (either proportion of completely healed wounds or time to complete healing was the primary endpoint. Data collection and analysis: Two review authors independently carried out trial selection, data extraction and risk of bias assessment, checked by a third review author.MAIN RESULTS:Seven trials were eligible for inclusion, comprising a total of 347 participants. Five trials in people with acute wounds evaluated the effects of Aloe vera on burns, haemorrhoidectomy patients and skin biopsies. Aloe vera mucilage did not increase burn healing compared with silver sulfadiazine (risk ratio (RR 1.41, 95% confidence interval (CI 0.70 to 2.85. A reduction in

  2. Formulation and Characterization of Aceclofenac -Aloe vera Transemulgel.

    Science.gov (United States)

    Raju, Y Prasanna; Haritha, K; Satyanarayana, Rao P; Vandana, K R; Bindu, D Thushara; Vinesha, V; Chowdary, V Harini

    2015-01-01

    The present research was aimed to formulate aceclofenac transemulgel using Aloe vera as gel base. The prepared formulations were subjected to physical characterization, in-vitro and in-vivo assessment. Aceclofenac, a hydrophobic potential non steroidal anti inflammatory drug, causes ulceration upon chronic oral administration, could be formulated into transemulgel to enhance therapeutic efficacy and to lower the unwanted side effects. The transemulgel was prepared from aqueous Aloe vera gel and aceclofenac emulsion. The prepared transemulgel was evaluated for its pH, viscosity, drug content, skin irritation, in-vitro diffusion and accelerated stability studies. The prepared aceclofenac-Aloe vera tranemulgel and commercial aceclofenac gel were subjected to pharmacodynamic studies in albino rats of Wistar strain employing carrageenan induced left hind paw edema method to assess the anti-inflammatory effect. The transemulgel showed a pH of 6.78 and viscosity of 18 cps. In-vitro diffusion data revealed better permeation characteristics. Topical application of formulation found no skin irritation. Stability study has proved the integrity of the formulation. The prepared aceclofenac Aloe vera transemulgel showed better in-vitro drug release when compared with the commercial aceclofenac gel formulation. Anti-inflammatory activity in treated rats showed the significant paw volume reduction at pAloe vera as gel base.

  3. Hemlock alkaloids from Socrates to poison aloes.

    Science.gov (United States)

    Reynolds, Tom

    2005-06-01

    Hemlock (Conium maculatum L. Umbelliferae) has long been known as a poisonous plant. Toxicity is due to a group of piperidine alkaloids of which the representative members are coniine and gamma-coniceine. The latter is the more toxic and is the first formed biosynthetically. Its levels in relation to coniine vary widely according to environmental conditions and to provenance of the plants. Surprisingly, these piperidine alkaloids have turned up in quite unrelated species in the monocotyledons as well as the dicotyledons. Aloes, for instance, important medicinal plants, are not regarded as poisonous although some species are very bitter. Nevertheless a small number of mostly local species contain the alkaloids, especially gamma-coniceine and there have been records of human poisoning. The compounds are recognized by their characteristic mousy smell. Both acute and chronic symptoms have been described. The compounds are neurotoxins and death results from respiratory failure, recalling the effects of curare. Chronic non-lethal ingestion by pregnant livestock leads to foetal malformation. Both acute and chronic toxicity are seen with stock in damp meadows and have been recorded as problems especially in North America. The alkaloids derive biosynthetically from acetate units via the polyketide pathway in contrast to other piperidine alkaloids which derive from lysine.

  4. Significación clínica y determinantes de patogenicidad de "Staphylococcus" coagulasa negativos aislados en hemocultivos

    OpenAIRE

    Hidalgo Orozco, María del Rocío

    2016-01-01

    Los Staphylococcus coagulasa negativa (SCN) son los microorganismos aislados con mayor frecuencia en hemocultivos. Suelen ser contaminantes en la mayoría de las ocasiones, ya que son parte de la flora habitual de la piel, aunque también está demostrado que son causantes de bacteriemias. Son muchos los autores que han propuesto una serie de factores clínicos y de laboratorio que sirvan de ayuda en la determinación de la significación clínica de los SCN. En la práctica clínica, si solo un hem...

  5. Sensibilidad a azitromicina y otros antibióticos en aislados recientes de Salmonella, Shigella y Yersinia

    OpenAIRE

    Martín Pozo, Ángeles; Arana, David M.; Fuentes, Miriam; Alós Cortés, Juan Ignacio

    2014-01-01

    La azitromicina constituye una alternativa antibiótica en la diarrea bacteriana. Existen pocos datos en España de sensibilidad a azitromicina de enteropatógenos. Se determinaron las CMI de azitromicina por E-test en aislados de Salmonella no typhi (SNT), Shigella y Yersinia de los últimos 3 años (2010-2012). También se estudió la sensibilidad a los antibióticos habitualmente utilizados en la clínica diarreica mediante un método de microdilución. De las 139 cepas de SNT, Shigella y Yer...

  6. ALOE VERA: A REVIEW OF ITS CLINICAL EFFECTIVENESS

    Directory of Open Access Journals (Sweden)

    Malik Itrat

    2013-08-01

    Full Text Available Aloe vera has been used for over five thousand years. Throughout history, it has been considered a magical plant, almost a panacea, capable of remedying many of mankind’s ailments. It is only in the last 20 years, after a series of proven research, that we can highlight the characteristics of this plant, whose secrets have been hidden behind a blanket of botanical and pharmacological puzzles that only today begin to yield some answers. The Aloe vera plant and its clinical uses are briefly reviewed in this article.

  7. Caracterización molecular de la región determinante de resistencia a quinolonas (QRDR de la topoisomerasa IV de Bartonella bacilliformis en aislados clínicos

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    Abraham Espinoza-Culupú

    2014-05-01

    Full Text Available Bartonella bacilliformis es el agente etiológico de la Enfermedad de Carrión, endémica del Perú. Pocas investigaciones han sido realizadas acerca de los genes asociados a la resistencia antimicrobiana en aislados clínicos de este patógeno. Estos genes no están caracterizados molecularmente, ni se conoce la región asociada a dicha resistencia. Por ello, el objetivo del este trabajo fue caracterizar molecularmente la región determinante de la resistencia a las quinolonas (QRDR en la topoisomerasa IV, que está codificada por los genes parC y parE, así como también desarrollar una prueba de susceptibilidad antimicrobiana para B. bacilliformis. Las muestras sanguíneas de 65 pacientes procedentes de La Libertad, Cusco, Ancash y Piura, se sembraron en placas de agar sangre e incubaron a 30 °C con 5% CO2. Luego se procedió a: (1 determinar la susceptibilidad antimicrobiana y (2 extraer el DNA genómico, amplificar los genes mencionados, secuenciarlos y analizarlos mediante herramientas bioinformáticas. Se obtuvieron 6 cultivos positivos. Los aislados fueron sensibles a la ciprofloxacina (excepto uno procedente de Quillabamba-Cusco, que presentó susceptibilidad disminuida y resistentes al ácido nalidíxico. Del análisis de las secuencias aminoacídicas de ParC y ParE de B. bacilliformis se concluye que presentan diferencias aminoacídicas en comparación con las secuencias de las proteínas respectivas de E. coli K12 MG1655, que probablemente confieran resistencia al ácido nalidíxico pero no a la ciprofloxacina. Se determinó que las QRDR de las proteínas ParC y ParE de B. bacilliformis están comprendidas entre los aminoácidos 67 al 118 y 473 al 530, respectivamente. El antibiograma y la concentración mínima inhibitoria se evalúan mejor usando inóculos a escala 1 de McFarland y a los 6 días de incubación.

  8. Separation of Water to Concentrate Aloe Vera Juice

    Directory of Open Access Journals (Sweden)

    Ishfaq Abdullah

    2014-11-01

    Full Text Available Aloe Vera is a succulent plant and is found in different arid areas. Due to its healing effect and soothing properties it is being used in herbal medicines for years and its importance has grown due to use in cosmetic products. It contains more than thirty active ingredients, which are utilized in cosmetic and pharmaceutical industry. It also contains 98% water. To remove water from those active ingredient Reverse osmosis is used in order not to thermally damage the active ingredients present in the juice. A reverse osmosis is a separation technique which utilizes difference in pressure to segregate water from plant without damaging the active ingredients present in the plant. This research is dedicated to evaluate the parameter for separation of water from aloe Vera so that aloe Vera can be obtained in a form which can be utilized for different purposes such as cosmetics and medicine. Osmosis technique is preferred over other conventional technique for the separation purpose because it is not operated at high temperature, which will not damage the aloe Vera juice quality. Next step in this research is the preserving, drying and analysis of this juice so that this product can be utilized in versatile way.

  9. Immunomodulatory properties of aloe vera gel in mice

    Directory of Open Access Journals (Sweden)

    Madan Jyotsana

    2008-01-01

    Full Text Available Administration of Aloe vera extract to swiss albino mice (300 mg/kg i.p. daily for five days, significantly (P < 0.01 increases the total white blood cells count. Further, it increases humoral immune response, as demonstrated from the increase in plaque-forming cells in the spleen and circulating antibody titre.

  10. Oral Aloe vera as a treatment for osteoarthritis: a summary.

    Science.gov (United States)

    Cowan, David

    2010-06-01

    While pain relief is a basic tenet of health care, pain is under-treated in the UK (Davies and Mcvicar, 2000) and this issue remains unresolved. This paper suggests that oral Aloe vera could be used in the treatment of chronic non-cancer pain (CNCP), particularly that caused by osteoarthritis (OA). Despite being used as arthritis treatment for centuries (Yoo et al, 2008), evidence of effectiveness of Aloe vera is anecdotal or from small studies. The perceived benefits of prescribing Aloe vera for OA may be twofold: it has utility as an anti-inflammatory agent and also as a prophylactic against the gastrointestinal irritant effects of non-steroidal anti-inflammatory drugs (NSAIDs). Long-term, randomized, controlled studies are still needed to address the lack of evidence informing optimum prescribing of pain medication for people with OA (Cowan, 2007). There is no reason that so called 'nutraceutical' agents should not be subjected to the same rigorous randomized, controlled, double-blind trials as other 'mainstream' drugs. Therefore, it is appropriate to ask whether NSAID treatment and side effects can be improved by the addition of oral Aloe vera. Thus, we may then be in a more informed position to resolve the ongoing 'Pandemonium over Painkillers' (Cowan, 2007).

  11. Ribosomal DNA evolution and phylogeny in Aloe (Asphodelaceae).

    Science.gov (United States)

    Adams, S P; Leitch, I J; Bennett, M D; Chase, M W; Leitch, A R

    2000-11-01

    All Aloe taxa (∼400 species) share a conserved bimodal karyotype with a basic genome of four large and three small submetacentric/acrocentric chromosomes. We investigated the physical organization of 18S-5.8S-26S and 5S ribosomal DNA (rDNA) using fluorescent in situ hybridization (FISH) to 13 Aloe species. The organization was compared with a phylogenetic tree of 28 species (including the 13 used for FISH) constructed by sequence analysis of the internal transcribed spacer (ITS) of 18S-5.8S-26S rDNA. The phylogeny showed little divergence within Aloe, although distinct, well-supported clades were found. FISH analysis of 5S rDNA distribution showed a similar interstitial location on a large chromosome in all species examined. In contrast, the distribution of 18S-5.8S-26S rDNA was variable, with differences in number, location, and size of loci found between species. Nevertheless, within well-supported clades, all species had the same organizational patterns. Thus, despite the striking stability of karyotype structure and location of 5S rDNA, the distribution of 18S-5.8S-26S rDNA is not so constrained and has clearly changed during Aloe speciation.

  12. Trypanosoma lambrechti n. sp. aislado de micos (cebus albifrons) de colombia

    OpenAIRE

    Marinkelle, C. J.

    2012-01-01

    En un estudio en sangre de micos colombianos, pertenecientes a 13 especies diferentes, 3 de 12 Cebus albifrons hospedaban en su sangre periférica un número muy bajo de Trypanosoma lambrechti n. sp.  La longitud promedio del tripanosoma, incluyendo la porción libre del fIagelo, es 34.9 micrones (30.1 - 43.2 micrones). EI cinetoplasto submarginal es muy pequeño y se sitúa en la cercanía del núcleo. EI núcleo es pequeño, oval o en forma de banda y ocupa la mayor parte de la anchura del c...

  13. Evaluation of in vitro and in vivo antioxidant potential of polysaccharides from Aloe vera (Aloe barbadensis Miller) gel.

    Science.gov (United States)

    Kaithwas, Gaurav; Singh, Prashant; Bhatia, Daksh

    2014-04-01

    In the present study, the antioxidant activity of the polysaccharides from aloe vera (Aloe barbadensis Miller) gel was evaluated, in vitro by five established methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH(-)) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide scavenging, superoxide radical (O(-2)) scavenging and reducing power assay, and in vivo against doxorubicin (DOX)-induced myocardial oxidative stress (OS) in albino wistar rats. The polysaccharides exhibited significant inhibitory activity against DPPH(-), superoxide, NO and hydrogen peroxide scavenging assay with significant reducing activity at all concentrations used. DOX-induced (7.5 mg/kg, intravenously) cardiotoxicity manifested biochemically by a significant decrease in blood and tissue glutathione (GSH) along with elevated levels of serum lactate dehydrogenase and creatine phosphokinase. In addition, cardiotoxicity was further confirmed by the significant increase in lipid peroxidation expressed as thiobarbituric acid reactive substances (TBARS), catalase (CAT) and superoxide dismutase (SOD). Administration of aloe vera polysaccharides for 14 days produced a marked protection against cardiotoxicity induced by DOX evidenced by significant reductions in serum lactate dehydrogenase, serum creatine phosphokinase, cardiac TBARS, CAT and SOD along with increased levels of blood and tissue GSH in a dose-dependent manner. The present investigation is the first to establish the antioxidant potency of the polysaccharides from aloe vera against DOX-induced myocardial OS.

  14. Determinación del mecanismo anti-inflamatorio de peniocerol y chichipegenina aislados de Myrtillocactus geometrizans /

    OpenAIRE

    Rodrigo Salazar, Juan

    2011-01-01

     tesis que para obtener el grado de Doctor en Ciencias Biomédicas, presenta Juan Rodrigo Salazar ; asesor Mariano Martínez Vázquez. 172 páginas : ilustraciones, diagramas. Doctorado en Ciencias Biomédicas UNAM, Instituto de Química, 2011

  15. Efficacy of orally administered powdered aloe juice (Aloe ferox against ticks on cattle and ticks and fleas on dogs

    Directory of Open Access Journals (Sweden)

    J.J. Fourie

    2005-06-01

    Full Text Available The efficacy of orally administered powdered aloe juice (Aloe ferox was evaluated against ticks on cattle and against ticks and fleas on dogs. Twelve calves were each infested over a 25-day period with approximately 4000 larvae of Rhipicephalus (Boophilus decoloratus and allocated to 3 groups of 4 calves each. Three days after the last larval infestation and daily for 22 days thereafter, the calves in 1 group were fed 5 mg / kg body weight and those in another 25 mg / kg body weight of powdered aloe juice incorporated in game maintenance pellets, while the animals in the 3rd group received only pellets. Detached female ticks were collected daily and counted and the weights and the fertility of groups of 50 engorged female ticks collected from the animals were ascertained. The powdered aloe juice in the game maintenance pellets had no effect on the tick burdens of the calves or on the fertility of the ticks. Six dogs, in each of 2 groups, were treated daily for 15 consecutive days, commencing on Day -5 before the 1st tick infestation, with either 0.39 g or 0.74 g of powdered aloe juice, administered orally in gelatin capsules, while a 3rd group of 6 dogs served as untreated controls. All the dogs were challenged with Haemaphysalis leachi on Days 0 and +7, and with Ctenocephalides felis on Days+1and +8, and efficacy assessments were made 1 day after flea and 2 days after tick challenge, respectively. Treatment was not effective against ticks or fleas on the dogs.

  16. Aloe Gel Enhances Angiogenesis in Healing of Diabetic Wound

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    Djanggan Sargowo

    2011-12-01

    Full Text Available BACKGROUND: Diabetic micro and macroangiophathy lead to the incident of diabetic foot ulcers characterized by an increased number of circulating endothelial cells (CECs and decreased function of endothelial progenitor cells (EPCs. This fact is correlated with ischemia and diabetic wound healing failure. Aloe vera gel is known to be able to stimulate vascular endothelial growth factor (VEGF expression and activity by enhancing nitric oxide (NO production as a result of nitric oxide synthase (NOS enzyme activity. Aloe vera is a potential target to enhancing angiogenesis in wound healing. OBJECTIVE: The objective of this study was to explore the major role of Aloe vera gel in wound healing of diabetic ulcers by increasing the level of EPCs, VEGF, and endothelial nitric oxide synthase (eNOS, as well as by reducing the level of CECs involved in angiogenesis process of diabetic ulcers healing. METHODS: The experimental groups was divided into five subgroups consisting of non diabetic wistar rats, diabetic rats without oral administration of aloe gel, and treatment subgroup (diabetic rats with 30, 60 and 120 mg/day of aloe gel doses for 14 days. All subgroups were wounded and daily observation was done on the wounds areas. Measurement of the number of EPCs (CD34, and CECs (CD45 and CD146 was done by flow cytometry, followed by measurement of VEGF and eNOS expression on dermal tissue by immunohistochemical method on day 0 and day 14 after treatment. The quantitative data were analyzed by One-Way ANOVA and Linear Regression, with a confidence interval 5% and significance level (p<0.05 using SPSS 16 software to compare the difference and correlation between wound diameters, number of EPCs and CECs as well as the levels of VEGF and eNOS. RESULTS: The results of this study showed that aloe gel oral treatment in diabetic wistar rats was able to accelerate the wound healing process. It was shown by significant reduction of wound diameter (0.27±0.02; the

  17. The Genus Aloe: Phytochemistry and Therapeutic Uses Including Treatments for Gastrointestinal Conditions and Chronic Inflammation.

    Science.gov (United States)

    Cock, I E

    2015-01-01

    Plants of the genus Aloe have perhaps the longest recorded history of medicinal usage and are amongst the most widely used plants for traditional medicinal purposes worldwide. Aloe vera, Aloe ferox, Aloe arborescens and Aloe perryi are the best known and most widely used, but many other species are also used for their therapeutic properties. The Aloes have been used since ancient times, particularly for the treatment of microbial infections, gastrointestinal disorders and inflammatory conditions. In addition to their myriad uses in traditional therapeutics, the Aloes have also been used as components of cosmetic formulations, and in the food and beverage industries. Despite their wide acceptance, studies from different laboratories often report wide variations in the therapeutic bioactivities from within the same Aloe species, even when the same extraction procedures are used. Furthermore, leaves from individual Aloe plants within the same species may have widely varying levels of the bioactive phytochemicals. Phytochemical analyses have shown that many Aloe species contain various carbohydrate polymers (notably glucomannans) and a range of other low molecular weight phenolic compounds including alkaloids, anthraquinones, anthrones, benzene and furan derivatives, chromones, coumarins, flavonoids, phytosterols, pyrans and pyrones. There has been a wealth of information published about the phytochemistry and therapeutic potential of the Aloes (especially Aloe vera). Much of this has been contradictory. Intra- and interspecies differences in the redox state of the individual Aloe components and in the ratios of these components may occur between individual plants. These factors may all affect the physiological properties of Aloe extracts. Due to the structure and chemical nature of many of the Aloe phytochemicals, it is likely that many of the reported medicinal properties are due to antioxidant or prooxidant effects. The antioxidant/prooxidant activities of many Aloe

  18. SINTESIS MEMBRAN NATA ALOE VERA-ETILENDIAMIN DAN KARAKTERISASINYA

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    EB Susatyo

    2014-06-01

    Full Text Available Serat yang terkandung di dalam nata Aloe vera adalah selulosa sehingga dapat digunakan dalam sintesis membran. Telah dilakukan penelitian tentang sintesis membran nata Aloe vera-etilendiamin (nata-en menggunakan sistem vakum cair. Proses preparasi membran berlangsung melalui tiga tahap, yaitu preparasi nata Aloe vera, aktivasi menggunakan asam sulfat, dan modifikasi dengan menggunakan etilendiamin. Tujuan penelitian adalah untuk mempelajari teknik dalam sintesis membran nata-en kemudian melakukan karakterisasi untuk mengetahui karakter strukturnya. Hasil penelitian menunjukkan perilaku yang berbeda dalam hal sifat mekanik dan strukturnya. Membran nata murni memiliki sifat mekanik yang kuat, nata teraktivasi cenderung rapuh, sedangkan nata-en bersifat liat. Spektra infra merah dari ketiga tipe membran (nata murni, nata teraktivasi, dan nata-en secara umum tidak mengalami perubahan yang signifikan, hanya terjadi pergeseran panjang gelombang dari masing-masing membran. Berdasarkan spektra infra merah dapat diketahui bahwa masing-masing membran mempunyai gugus hidroksil, tetapi serapannya semakin melebar untuk setiap membran. Gugus alkil dan karboksil juga masih tampak, namun pada membran nata teraktivasi serapannya berkurang, sedangkan pada membran nata-en muncul puncak baru yang menunjukkan adanya gugus amin. Hal ini membuktikan bahwa telah terjadi ikatan antara nata dengan etilendiamin. Fibers contained in nata Aloe vera is cellulose that can be used in the synthesis of membrane. The research has done on the synthesis of nata Aloe vera-ethylenediamine (nata-en membrane by using liquid vacuum system. Membrane preparation process consisted of three stages, they are nata Aloe vera preparation, activation using sulfuric acid, and membrane modification by using ethylenediamine. The purpose of research is to study the technique of nata-en membrane synthesis then to perform the characterization to determine the character of their structure.The results

  19. Acute hepatitis induced by an Aloe vera preparation: A case report

    Institute of Scientific and Technical Information of China (English)

    Christian Rabe; Annemarie Musch; Peter Schirmacher; Wolfgang Kruis; Robert Hoffmann

    2005-01-01

    AIM: Aloe vera, plant extracts of Aloe barbadensis miller,is widely used in phytomedicine. The first case of acute hepatitis due to this compound was described.METHODS: Description of a clinical case.RESULTS: Hepatitis in a 57-year old female could be linked to the ingestion of Aloe barbadensis miller compounds. The patient's hepatitis resolved completely after discontinuing this medication.CONCLUSION: The case emphasizes the importance of considering phytopharmaceutical over-the-counter drugs as causative agents of hepatitis.

  20. The Review on Properties of Aloe Vera in Healing of Cutaneous Wounds.

    Science.gov (United States)

    Hashemi, Seyyed Abbas; Madani, Seyyed Abdollah; Abediankenari, Saied

    2015-01-01

    Treatment of wounds is very important and was subject of different investigations. In this regard, natural substance plays crucial role as complementary medicine. Various studies reported that aloe vera has useful effects on wounds especially cutaneous wounds healing. Therefore in the current review, we examined the effect of aloe vera on cutaneous wound healing and concluded that although aloe vera improves the wound healing as well as other procedures both clinically and experimentally, more studies are still needed to approve the outcomes.

  1. Effectivity of Aloe vera bioactives as feed additive for broilers reared on deep litter

    OpenAIRE

    A.P Sinurat; T Purwadaria; T Pasaribu; W Rakhmani; J Dharma; J Rosida; S Sitompul; Udjianto

    2004-01-01

    There are plenty of Indonesian plants contain usefull bioactive components. One of them is Aloe vera. Previous experiment showed that Aloe vera bioactives reduced aerob bacteria in the intestinal and improved feed efficiency in broilers reared in cages. The results however, gave some variations, may be due to variation in rearing the chickens. Two experiments were carried out to study the effectivity of Aloe vera bioactives as feed additives for broilers reared on deep litter. In the first st...

  2. Development of Aloe vera based edible coating for tomato

    Science.gov (United States)

    Athmaselvi, K. A.; Sumitha, P.; Revathy, B.

    2013-12-01

    The effect of formulated Aloe vera based edible coating on mass loss, colour, firmness, pH, acidity, total soluble solid, ascorbic acid and lycopene on the coated tomato was investigated. The tomato in control showed a rapid deterioration with an estimated shelf life period of 19 days, based on the mass loss, colour changes, accelerated softening and ripening. On the contrary, the coating on tomatoes delayed the ripening and extended the shelf life up to 39 days. The physiological loss in weight was 7.6 and 15.1%, firmness was 36 and 46.2 N on 20th day for control and coated tomatoes, respectively. From the results, it was concluded that the use of Aloe vera based edible coating leads to increased tomato shelf-life.

  3. Memristors in the electrical network of Aloe vera L.

    Science.gov (United States)

    Volkov, Alexander G; Reedus, Jada; Mitchell, Colee M; Tucket, Clayton; Forde-Tuckett, Victoria; Volkova, Maya I; Markin, Vladislav S; Chua, Leon

    2014-01-01

    A memristor is a resistor with memory, which is a non-linear passive two-terminal electrical element relating magnetic flux linkage and electrical charge. Here we found that memristors exist in vivo. The electrostimulation of the Aloe vera by bipolar sinusoidal or triangle periodic waves induce electrical responses with fingerprints of memristors. Uncouplers carbonylcyanide-3-chlorophenylhydrazone and carbonylcyanide-4-trifluoromethoxy-phenyl hydrazone decrease the amplitude of electrical responses at low and high frequencies of bipolar periodic sinusoidal or triangle electrostimulating waves. Memristive behavior of an electrical network in the Aloe vera is linked to the properties of voltage gated ion channels: the K(+) channel blocker TEACl reduces the electric response to a conventional resistor. Our results demonstrate that a voltage gated K(+) channel in the excitable tissue of plants has properties of a memristor. The discovery of memristors in plants creates a new direction in the modeling and understanding of electrical phenomena in plants.

  4. Processing, food applications and safety of aloe vera products: a review.

    Science.gov (United States)

    Ahlawat, Kulveer Singh; Khatkar, Bhupender Singh

    2011-10-01

    Aloe vera is used for vigor, wellness and medicinal purposes since rigvedic times. Health benefits of aloe vera include its application in wound healing, treating burns, minimizing frost bite damage, protection against skin damage from x-rays, lung cancer, intestinal problems, increasing high density lipoprotein (HDL), reducing low density lipoprotein (LDL), reducing blood sugar in diabetics, fighting acquired immune deficiency syndrome (AIDS), allergies and improving immune system. Phytochemistry of aloe vera gel has revealed the presence of more than 200 bioactive chemicals. Aloe vera gel is extracted from its leaves and appropriate processing techniques are needed for stabilization as well as preparation of the end products. The industries involved in processing of aloe vera need Government surveillance to ensure that the aloe vera products have beneficial bio-active chemicals as per claims of the manufacturers. Regulatory bodies also need to look into the safety and toxicological aspects of aloe vera products for food applications. The claims made for medicinal value of aloe products should be supported by authentic and approved clinical trial data. It is presumptive to mention that nutraceutical claims of aloe products made by the manufacturers are numerous. However, approved clinical evidences are available only for lowering LDL, increasing HDL, decreasing blood glucose level, treating genital herpes and psoriases.

  5. The effect of Aloe vera bioactive and anthraquinone on the performance of laying hens

    OpenAIRE

    Tiurma Pasaribu; A.P Sinurat; Susana I.W Rakhman

    2005-01-01

    An experiment was conducted to study the use of Aloe vera bioactives as feed additives on the performances of laying hens. The Aloe vera bioactives was prepared as the dry gel (DG) and semi liquid gel (SLG). The Aloe vera was suplemented into the diets with concentration of equal to 0.5 and 1.0 g DG/kg diets. Diets contained commercial anthraquinone, a bioactive compound of Aloe vera with doses equal to 0.5 g DG and 1.0 dg/kg were also prepared. Diets were compared to control diets containing...

  6. Caracterización de actinobacterias raras, degradadoras de lignocelulosa: demostración de actividad lacasa en dos aislados de Tsukamurella sp y Cellulosimicrobium sp

    Directory of Open Access Journals (Sweden)

    Enrique Luis Revollo Escudero

    2014-01-01

    Full Text Available Título en ingles: Characterization of lignocelluloses-degrading rare actinobacteria: Demostration of laccase activity in two isolates of Tsukamurella sp and Cellulosimicrobium sp Resumen: Las características fisicoquímicas de la lignina y su compactación con la celulosa han dificultado la explotación biotecnológica de enormes cantidades de biomasa vegetal. Las lacasas constituyen una subfamilia de oxidasas multicobre que intervienen en la despolimerización de la lignina. Si bien han sido ampliamente caracterizadas en los hongos, los estudios de la diversidad y las funcionalidades de las lacasas en los procariotas se han centrado especialmente en isoformas enzimáticas de Streptomyces sp. En este trabajo se aislaron 20 cepas de actinobacterias del suelo. La actividad lacasa de 17 de ellas fue evidenciada en ensayos cualitativos con guayacol y dos cepas seleccionadas fueron caracterizadas en detalle. Las pruebas morfológicas y el análisis de las secuencias del gen 16S rRNA apuntan a que estos dos aislados pertenecen a los géneros Tsukamurella y Cellulosimicrobium. En cultivo sumergido con agitación, AC01 (Tsukamurella sp. expresó una máxima actividad de oxidación de ABTS (2,2’-azino-bis-(3-etilbenzotiazolin-6-sulfonato de 108 U/L. Por otra parte, AC18 (Cellulosimicrobium sp. que había exhibido una actividad oxidativa de guayacol superior a las 16 cepas restantes y demostró ser resistente a niveles tóxicos de cobre, logró un valor máximo de oxidación del ABTS de 0,56 U/L. Estos resultados sugieren que en el aislado AC18 operaría un fenómeno de especificidad de sustrato o de inductor, regulador de la expresión y de la actividad lacasa cuantificable. La caracterización genómica y funcional de las lacasas de nuevas actinobacterias lignocelulósicas ampliará la gama de centros redox con aplicaciones biotecnológicas específicas, además de facilitar el establecimiento de sus relaciones evolutivas con las eucariotas

  7. Gaia16alo is a Type II SN

    Science.gov (United States)

    Fraser, M.; Hodgkin, S. T.; Mattila, S.; Harrison, D.; Wyrzykowski, L.; Kostrzewa-Rutkowska, Z.; Blagorodnova, N.

    2016-05-01

    Gaia16alo (aka PS16cct) was observed using the robotic Liverpool Telescope + SPRAT (R~350; 400-800 nm) on the night of 2016 May 6. The spectrum was compared to a set of templates using SNID (Blondin & Tonry, 2007, ApJ, 666, 1024), and we find a best match to a range of Type II SNe at z=0.03.

  8. Encapsulation of Aloe Vera and Its Effect During Yogur Incubation

    Directory of Open Access Journals (Sweden)

    Ricardo Adolfo Parra Huertas

    2014-11-01

    Full Text Available The yogurt is milk derivative highly consumed around the world,as well as aloe vera. Both have reports tocontribute to human health. The purpose of this research is to determine the effect of the addition of capsules with aloe vera during the incubation of yogurt. Aloeverawas encapsulated in alginate at two different concentrations, 1% and 2%,addingthe capsules from the moment of incubation and comparing the effect of the addition of capsules withthe non-addition of them. For these samples were determined: pH, acidity, syneresis, lactic acid bacteria count, sensory evaluation and proximate analysis. The results indicated that for the three treatments pH values and acid behaved similarly to each characteristic of the yogurt during incubation. The lactic acid bacteria count indicated that treatment with capsules containing 2% sodium alginate had higher counts. Sensorially, three treatments had a favorable acceptability; proximate analysis had favorable values . In conclusion,the tests showed the viability of encapsulated aloe vera in the manufacture of yogurt during incubation time without being affected by the concentration of sodium alginate.

  9. Comparative evaluation of the effect of chlorhexidine and Aloe barbadensis Miller (Aloe vera) on dentin stabilization using shear bond testing

    OpenAIRE

    Dakshita Joy Sinha; Natasha Jaiswal; Agrima Vasudeva; Paridhi Garg; Shashi Prabha Tyagi; Priyanka Chandra

    2016-01-01

    Introduction: The main objective of adhesive dentistry is to create an effective, durable union between the tooth structure and restorative material. However, degradation of adhesive dentine interface remains largely responsible for the relatively short lifetime of tooth colored resin restoration. Aim: The aim of the study is to compare the dentin collagen stabilization property of Chlorhexidine (CHX) and Aloe barbadensis Miller using shear bond strength testing. Materials and Methods: ...

  10. Cirugía de reemplazo valvular aórtico aislado en pacientes octogenarios: evaluación, riesgo operatorio y resultados a mediano plazo

    Directory of Open Access Journals (Sweden)

    Fernando Piccinini

    2010-01-01

    Full Text Available RESUMENIntroducciónLas indicaciones y los beneficios del reemplazo valvular aórtico han sido claramente establecidos, aun para poblaciones añosas, de aumentada comorbilidad. Aun así, la manipulación de ateromas aórticos, el uso de circulación extracorpórea y de clampeo aórtico, el paro cardíaco y la descalcificación ligadas a esta técnica y sus eventuales consecuencias generan dudas acerca de su indicación en este grupo de pacientes.ObjetivosComunicar la morbimortalidad del procedimiento en octogenarios y validar la utilidad de los puntajes de predicción de riesgo utilizados más frecuentemente.Material y métodosSe estudiaron 87 pacientes octogenarios sometidos a reemplazo aórtico aislado; se analizaron variables preoperatorias, intraoperatorias y posoperatorias y se aplicó el Euroscore; para facilitar el subanálisis del Euroscore logístico, la población se dividió en tres grupos: riesgo bajo, moderado y alto. Se utilizó técnica quirúrgica convencional. Para el seguimiento, se analizó la historia clínica y/o se realizó encuesta telefónica.ResultadosLa mediana de edad fue de 83 ± 2,5 años, con un rango de 80 a 89 años. El 60% de la población en estudio era de sexo femenino. La indicación quirúrgica fue estenosis 92%, endocarditis activa 4,6% y enfermedad valvular 3,4%. Antecedentes: hipertensión 71%, tabaquismo 31%, dislipidemia 39%, diabetes 11,5%, EPOC 10%, IRC-diálisis 2,3%, ACV 11,5%, IAM previo 8%, fibrilación auricular 16%, reoperación 15%. Cuadro clínico: asintomático 3,45%, angina crónica estable 10,3%, angina inestable 11%, ICC 13,8%, disnea CF III-IV 60%. Disfunción ventricular izquierda moderada-grave 18,3%. Riesgo por Euroscore logístico 12,4% ± 15%. El tiempo operatorio promedio fue de 200 ± 61,7 min, el de CEC de 86 ± 32,5 min y el de clampeo aórtico de 65 ± 18,2 min. Complicaciones: sangrado médico 17,2%, reoperación sangrado 5,7%, bajo gasto cardíaco 13,8%, inotrópicos > 48 horas

  11. Physicochemical characterization of silver nanoparticles synthesize using Aloe Vera (Aloe barbadensis)

    Science.gov (United States)

    Kuponiyi, Abiola; Kassama, Lamin; Kukhtareva, Tatiana

    2014-08-01

    Production of silver nanoparticles (AgNPs) using different biological methods is gaining recognition due to their multiple applications. Although, several physical and chemical methods have been used for the synthesis and stabilizing of AgNPs, yet, a green chemistry method is preferable because it is cost effective and environmentally friendly. The synthesis was done using Aloe Vera (AV) extract because it has chemical compounds such as "Antrokinon" that are known for its antibacterial, antivirus and anticancer properties. We hypothesize that AV extract can produce a stable nanoparticles within the 100 nm range and be biologically active. The biological compounds were extracted from AV skin with water and ethanol which was used as the reduction agent for the synthesis of nanoparticles. The biological extract and AgNO3 were blended and heated to synthesize AgNPs. The reaction process was monitored using UV-Visible spectroscopy. Fourier Transfer Infrared spectroscopy (FTIR) was used for the characterization of biological compounds and their substituent groups before and after the reaction process. Dynamic Light scattering (DLS) method was used to characterize particle size of AgNPs and their biomolecular stability. Results showed that biological compounds such as aliphatic amines, alkenes (=C-H), alkanes (C-H), alcohol (O-H) and unsaturated esters(C-O), which has an average particle size of 109 and 215.8 nm and polydispersity index of 0.451 and 0.375 for ethanol and water extract, respectively. According to TEM measurements the size of AgNPs are in the range 5-20 nm The results suggested that ethanol derived AgNPs contained higher yield of organic compounds, thus has better solubility power than water. Ag NPs can be used to control salmonella in poultry industry.

  12. Efectividad de una formulación hidrófila de Bixa orellana L. y Aloe vera L. en el tratamiento de quemaduras en terneros (Effectiveness of Bixa orellana L. and Aloe vera L. hydrophilic formulation in the treatment of burns in calves

    Directory of Open Access Journals (Sweden)

    Silveira Prado, Enrique A

    2011-01-01

    Full Text Available ResumenSe evaluó el efecto de una formulación hidrófila para el tratamiento de lasquemaduras en terneros, en que se incorporan como ingredientes activos unextracto de semillas de Bixa orellana L. y el gel de las hojas de Aloe vera L.En las condiciones del estudio, los animales tratados se recuperaron a los 4,5días promedio.SummaryThe effect of a hydrophilic formulation was evaluated for the treatment of theburns in calves which incorporates as active ingredients an extract of seeds ofBixa orellana L. and the gel of the leaves of Aloe vera L. Under the conditionsof the study, the treated animals recovered to the 4.5 days average.

  13. Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.

    Science.gov (United States)

    Tummalapalli, Mythili; Berthet, Morgane; Verrier, Bernard; Deopura, B L; Alam, M S; Gupta, Bhuvanesh

    2016-01-01

    Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices. The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix. A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings. However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings. The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells. OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation. On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells. In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice. OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days. Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention. Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei. Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.

  14. Morfometría Comparativa de Cinco Aislados Venezolanosde Trypanosoma vivax

    OpenAIRE

    Reyna Bello, Armando

    2014-01-01

    http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0258-65762014000100005 La tripanosomosis causada por Trypanosoma vivax, se encuentra distribuida en las regiones tropicales y subtropicales de África, América Latina y Asia, causando grandes pérdidas económicas. Los análisis biométricos previos reportan que T. vivax mide entre 18 y 31 µm; sin embargo, pocos estudios de este tipo se han realizado en Venezuela. El objetivo del presente estudio fue describir y comparar la morfometría...

  15. Morfometría Comparativa de Cinco Aislados Venezolanos de Trypanosoma vivax

    OpenAIRE

    Reyna Bello, Armando

    2014-01-01

    http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0258-65762014000100005 La tripanosomosis causada por Trypanosoma vivax, se encuentra distribuida en las regiones tropicales y subtropicales de África, América Latina y Asia, causando grandes pérdidas económicas. Los análisis biométricos previos reportan que T. vivax mide entre 18 y 31 µm; sin embargo, pocos estudios de este tipo se han realizado en Venezuela. El objetivo del presente estudio fue describir y comparar la morfometría...

  16. COMPUESTOS FENÓLICOS AISLADOS DE LA ESPECIE Solanum validinervium (Solanaceae SECCION GEMINATA

    Directory of Open Access Journals (Sweden)

    Luis Enrique Cuca Suárez

    2008-03-01

    Full Text Available A partir del extracto etanólico de la parte aérea de la especie Solanum validinervium  se aisló el compuesto 2-(4’-hidroxifenil-etanol, las cumarinas esculetina e isoescopoletina y un nuevo glucósido de cumarina identificado como 1’-O-7-esculetina-4’-O-1’’-etilenglicol-β-D-glucopiranosa. Sus estructuras fueron elucidadas por medio de técnicas espectroscópicas (IR y RMN-1H, RMN-13C  en una y dos dimensiones.

  17. Sistema de energía solar fotovoltaica aislado para vivienda unifamiliar aislada

    OpenAIRE

    2012-01-01

    El objetivo del presente Trabajo Fin de Grado es diseñar un sistema de energía solar fotovoltaica para una vivienda unifamiliar aislada de modo que sea autosuficiente energéticamente mediante un sistema fotovoltaico. Un sistema fotovoltaico completo, con gran capacidad de acumulación, puede garantizar un suministro fiable hasta varios días con ausencia de sol, y con una inversión mucho inferior al coste de hacer llegar la red eléctrica al emplazamiento. Para conseguir un sum...

  18. FLAVONOIDES CON ACTIVIDAD ANTIFÚNGICA AISLADOS DE Piper septuplinervium (Miq. C. DC. (Piperaceae

    Directory of Open Access Journals (Sweden)

    Mónica Avila

    2011-08-01

    Full Text Available El fraccionamiento bioguiado del extractoetanólico de la parte aérea de Piper septuplinervium(Piperaceae permitió la obtenciónde dos substancias de tipoflavonoide, activas contra dos cepas dehongos fitopatógenos (Fusarium oxysporumf. sp. dianthi y Botrytis cinerea. Lasestructuras de los compuestos aisladosfueron determinadas de acuerdo con elanálisis espectroscópico (RMN uni y bidimensional,EMAR. La actividad antifúngicafue determinada por ensayo endisco, seguido por bioautografía directasobre las dos cepas de hongos en prueba.

  19. METABOLITOS SECUNDARIOS AISLADOS DE LA CORTEZA DE Dugandiodendron argyrotrichum Lozano (MAGNOLIACEAE

    Directory of Open Access Journals (Sweden)

    Juan Guzmán

    2009-04-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 st1\\:*{behavior:url(#ieooui }   A partir del extracto etanólico de la corteza del árbol colombiano en peligro de extinción Dugandiodendron argyrotrichum (Magnoliaceae se aisló por cromatografía en columna un aceite compuesto mayoritariamente por sesquiterpenos, así como dos sesquiterpenoides, torreyol y partenólido, y  el alcaloide aporfínico N-acetilanonaína, por cromatografía en columna. Los compuestos se identificaron por cromatografía de gases acoplada a espectrometría de masas CG-MS y resonancia magnética nuclear (RMN de 1H y 13C. Los sustancias aisladas se sometieron al ensayo de letalidad en Artemia salina. Aquellos que resultaron más tóxicos fueron el aceite de sesquiterpenos (CL50 = 4.49 μg/ml y los lignanos dibencilbutánicos (1 (CL50 ~ 50 μg/ml.

  20. Investigación farmacoepidemiológica del consumo de antimicrobianos y de la sensibilidad de los microorganismos en Atención Primaria en un Área Sanitaria. Período 2004-2014

    OpenAIRE

    Rodríguez Álvarez, Francisco Javier

    2015-01-01

    Objetivos: Describir las tendencias en la prescripción de antimicrobianos y analizar su posible relación con de la sensibilidad a agentes antimicrobianos de los patógenos aislados en muestras de un Área Sanitaria. Material y métodos: Es un estudio descriptivo retrospectivo de la dispensación de los agentes antimicrobianos y de las tendencias de la susceptibilidad de los microorganismos aislados en muestras procedentes de Atención Primaria del Área Sanitaria III de Asturias, desde el año...

  1. Emodin and Aloe-Emodin Suppress Breast Cancer Cell Proliferation through ERα Inhibition

    Directory of Open Access Journals (Sweden)

    Pao-Hsuan Huang

    2013-01-01

    Full Text Available The anthraquinones emodin and aloe-emodin are abundant in rhubarb. Several lines of evidence indicate that emodin and aloe-emodin have estrogenic activity as phytoestrogens. However, their effects on estrogen receptor α (ERα activation and breast cancer cell growth remain controversial. The goal of this study is to investigate the effects and molecular mechanisms of emodin and aloe-emodin on breast cancer cell proliferation. Our results indicate that both emodin and aloe-emodin are capable of inhibiting breast cancer cell proliferation by downregulating ERα protein levels, thereby suppressing ERα transcriptional activation. Furthermore, aloe-emodin treatment led to the dissociation of heat shock protein 90 (HSP90 and ERα and increased ERα ubiquitination. Although emodin had similar effects to aloe-emodin, it was not capable of promoting HSP90/ERα dissociation and ERα ubiquitination. Protein fractionation results suggest that aloe-emodin tended to induce cytosolic ERα degradation. Although emodin might induce cytosolic ERα degradation, it primarily affected nuclear ERα distribution similar to the action of estrogen when protein degradation was blocked. In conclusion, our data demonstrate that emodin and aloe-emodin specifically suppress breast cancer cell proliferation by targeting ERα protein stability through distinct mechanisms. These findings suggest a possible application of anthraquinones in preventing or treating breast cancer in the future.

  2. Bacterial leaf rot of Aloe vera L., caused byErwinia chrysanthemi biovar 3

    NARCIS (Netherlands)

    Laat, de P.C.A.; Verhoeven, J.T.W.; Danse, J.D.

    1994-01-01

    A severe attack of the bacteriumErwinia chrysantemi biovar 3 on the succulentAloe vera on the Carribean island of Aruba is described. Biochemical and pathological characteristics of strains are presented, including results of successful inoculation experiments onAloe vera. This is the first report o

  3. DNA degradation by aqueous extract of Aloe vera in the presence of copper ions.

    Science.gov (United States)

    Naqvi, Shoa; Ullah, M F; Hadi, S M

    2010-06-01

    The plant Aloe vera has long been used in medicine, as dietary supplements and for cosmetic purposes. Aloe vera extracts are a rich source of polyphenols, such as aloin and aloe emodin and have shown a wide range of pharmacological properties, including anti-inflammatory and anti-cancer properties. The bioactive component aloe emodin has been reported to induce apoptosis in various cancer cell lines. Many of the biological activities of Aloe vera have been attributed to its antioxidant properties. However, most plant-derived polyphenols that are also present in Aloe vera may exhibit pro-oxidant properties either alone or in the presence of transition metals, such as copper. Previous reports from this laboratory have implicated the pro-oxidant action as one of the mechanisms for their anti-cancer properties. In the present paper, we show that aqueous extract of Aloe vera is also able to cause DNA degradation in the presence of copper ions. Further, the extract is also able to reduce Cu(II) to Cu(I) and generate reactive oxygen species, such as superoxide anion and hydroxyl radicals in a dose-dependent manner, which correlates with ability of the extract to cause DNA breakage. Thus, the study shows that in addition to antioxidant activity, Aloe vera extract also possess pro-oxidant properties, leading to oxidative DNA breakage.

  4. Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria.

    Science.gov (United States)

    Fani, Mohammadmehdi; Kohanteb, Jamshid

    2012-03-01

    Aloe vera is a medicinal plant with anti-inflammatory, antimicrobial, antidiabetic and immune-boosting properties. In the present study we investigated the inhibitory activities of Aloe vera gel on some cariogenic (Streptococcus mutans), periodontopathic (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis) and an opportunistic periodontopathogen (Bacteroides fragilis) isolated from patients with dental caries and periodontal diseases. Twenty isolates of each of these bacteria were investigated for their sensitivity to Aloe vera gel using the disk diffusion and microdilution methods. S. mutans was the species most sensitive to Aloe vera gel with a MIC of 12.5 µg/ml, while A. actinomycetemcomitans, P. gingivalis, and B. fragilis were less sensitive, with a MIC of 25-50 µg/ml (P Aloe vera gel at optimum concentration could be used as an antiseptic for prevention of dental caries and periodontal diseases.

  5. Biocontrol potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean.

    Science.gov (United States)

    Zhang, Fuli; Ge, Honglian; Zhang, Fan; Guo, Ning; Wang, Yucheng; Chen, Long; Ji, Xiue; Li, Chengwei

    2016-03-01

    Sclerotinia stem rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary is a major disease of soybean (Glycine max (L.) Merr.). At present, we revealed the three-way interaction between Trichoderma harzianum T-aloe, pathogen S. sclerotiorum and soybean plants in order to demonstrate biocontrol mechanism and evaluate biocontrol potential of T-aloe against S. sclerotiorum in soybean. In our experiments, T-aloe inhibited the growth of S. sclerotiorum with an efficiency of 56.3% in dual culture tests. T-aloe hyphae grew in parallel or intertwined with S. sclerotiorum hyphae and produced hooked contact branches, indicating mycoparasitism. Plate tests showed that T-aloe culture filtrate inhibited S. sclerotiorum growth with an inhibition efficiency of 51.2% and sclerotia production. T-aloe pretreatment showed growth-promoting effect on soybean plants. The activities of peroxidase, superoxide dismutase, and catalase increased, and the hydrogen peroxide (H2O2) as well as the superoxide radical (O2(-)) content in soybean leaves decreased after T-aloe pretreatment in response to S. sclerotiorum pathogen challenge. T-aloe treatment diminished damage caused by pathogen stress on soybean leaf cell membrane, and increased chlorophyll as well as total phenol contents. The defense-related genes PR1, PR2, and PR3 were expressed in the leaves of T-aloe-treated plants. In summary, T-aloe displayed biocontrol potential against S. sclerotiorum. This is the first report of unraveling biocontrol potential of Trichoderma Spp. to soybean sclerotinia stem rot from the three-way interaction between the biocontrol agent, pathogen S. sclerotiorum and soybean plants.

  6. Assessment of Anti HSV-1 Activity of Aloe Vera Gel Extract: an In Vitro Study

    Science.gov (United States)

    Rezazadeh, Fahimeh; Moshaverinia, Maryam; Motamedifar, Mohammad; Alyaseri, Montazer

    2016-01-01

    Statement of the Problem Herpes simplex virus (HSV) infection is one of the most common and debilitating oral diseases; yet, there is no standard topical treatment to control it. The extract of Aloe vera leaves has been previously reported to have anti-inflammatory, antibacterial, and also antiviral effects. There is no data on anti-Herpes simplex virus type 1 (HSV-1) activity of Aloe vera gel. Purpose This study aimed to evaluate the anti-HSV-1 activity of Aloe vera gel in Vero cell line. Materials and Method In this study, gel extraction and cytotoxicity of various increasing concentrations of Aloe vera gel (0.2, 0.5, 1, 2, and 5%) was evaluated in Dulbecco’s Modified Eagle Medium (DMEM) containing 2% fetal bovine serum (FBS). Having been washed with phosphate buffered saline, 50 plaque-forming units (PFU) of HSV-1 was added to each well. After 1 hour of incubation at 37°C, cell monolayers in 24 well plates were exposed to different increasing concentrations of Aloe vera gel. The anti-HSV-1 activity of Aloe vera gel in different concentrations was assessed by plaque reduction assays. Data were analyzed by using One-way ANOVA. Results The cytotoxicity assay showed that Aloe vera in prearranged concentrations was cell-compatible. The inhibitory effect of various concentrations of Aloe vera was observed one hour after the Vero cell was infected with HSV-1. However, there was no significant difference between two serial concentrations (p> 0.05). One-way ANOVA also revealed no significant difference between the groups. The findings indicated a dose-dependent antiviral effect of Aloe vera. Conclusion The findings showed significant inhibitory effect of 0.2-5% Aloe vera gel on HSV-1 growth in Vero cell line. Therefore, this gel could be a useful topical treatment for oral HSV-1 infections without any significant toxicity. PMID:26966709

  7. Acute toxic hepatitis caused by an aloe vera preparation in a young patient: a case report with a literature review.

    Science.gov (United States)

    Lee, Jeonghun; Lee, Mi Sun; Nam, Kwan Woo

    2014-07-01

    Aloe is one of the leading products used in phytomedicine. Several cases of aloe-induced toxic hepatitis have been reported in recent years. However, its toxicology has not yet been systematically described in the literature. A 21-year-old female patient was admitted to our hospital with acute hepatitis after taking an aloe vera preparation for four weeks. Her history, clinical manifestation, laboratory findings, and histological findings all led to the diagnosis of aloe vera-induced toxic hepatitis. We report herein on a case of acute toxic hepatitis induced by aloe vera.

  8. Gran Bretaña ante Europa. Tormenta en el Canal. El continente aislado

    Directory of Open Access Journals (Sweden)

    Elizalde, María Dolores

    2001-09-01

    Full Text Available Not available

    El presente artículo estudia el difícil camino de Gran Bretaña hacia la integración en la Comunidad Europea. El trabajo se inicia con un análisis de los factores que determinaron la posición británica ante Europa; explica luego el modelo de Europa deseado por los británicos; y se detiene especialmente en las distintas actitudes adoptadas por Gran Bretaña durante el proceso de integración europea, centrándose en tres ámbitos: la política, la economía y la defensa. En ese marco, el trabajo revisa las razones por las que Gran Bretaña apoyó o rechazó las distintas instituciones europeas que se fueron creando, los motivos de su alejamiento del núcleo inicial de la CEE, y las causas de su posterior incorporación a la estructura comunitaria.

  9. Frecuencia de genes que codifican factores de virulencia en Staphylococcus aureus aislados de niños que concurrieron al Hospital General Pediátrico Niños de Acosta Ñú, durante el año 2010

    Directory of Open Access Journals (Sweden)

    Fatima Rodríguez Acosta

    2015-04-01

    Full Text Available Staphylococcus aureus es un microorganismo con habilidad de infectar diferentes tejidos celulares, por portar genes que le confieren resistencia a antibióticos, factores de virulencia y su plasticidad genética, que podrían contribuir a una progresión rápida y complicada de la enfermedad. El Paraguay no cuenta con datos epidemiológicos que indiquen los factores de virulencia que presentan las cepas de S. aureus, por lo que el objetivo del trabajo fue determinar un perfil de virulencia detectando los genes codificantes de: hemolisinas α y β, enterotoxinas A, B, C, D, H y toxinas exfoliativas A y B. Este estudio observacional descriptivo de corte transverso, con muestreo no probabilístico de casos consecutivos, incluyó 50 aislados de S. aureus obtenidos a partir de muestras clínicas de secreciones de piel, partes blandas o líquidos corporales de pacientes menores de 17 años que concurrieron al Hospital General Pediátrico Niños de Acosta Ñú durante el año 2.010. Las reacciones de PCR incluyeron la detección de los genes: sea+seb+sec+ADNr16S, hlA+hlB, eta+etb, sed y seh. El 82% de los aislados provenía de niños que presentaron cuadros clínicos compatibles con infecciones de piel y partes blandas y el 18% de cuadros clínicos graves como sepsis, osteomielitis y neumonías. Los aislados contaban con datos de portación de Leucocidina de Panton-Valentine, el cual fue el factor de virulencia más frecuentemente detectado (58%, seguido de las hemolisinas alfa (16% y beta (8%. Las enterotoxinas y las toxinas exfoliativas fueron menos frecuentes (0-2%, y no se detectaron genes codificantes de las enterotoxinas C y D.

  10. Acemannan, an extracted polysaccharide from Aloe vera: A literature review.

    Science.gov (United States)

    Sierra-García, Gerardo Daniel; Castro-Ríos, Rocío; González-Horta, Azucena; Lara-Arias, Jorge; Chávez-Montes, Abelardo

    2014-08-01

    In this review, the composition, actions, and clinical applications of acemannan in medicine and its effectiveness as an adjunct in the treatment of diseases are presented. An electronic literature search was performed up to January 2014 for studies and research presenting data to validate the efficacy of acemannan. A total of 50 titles, abstracts and full-text studies were selected and reviewed. Acemannan has various medicinal properties like osteogenic, anti-inflammatory, and antibacterial, which accelerate healing of lesions. Also, acemannan is known to have antiviral and antitumor activities in vivo through activation of immune responses. It was concluded that Aloe vera has immense potential as a therapeutic agent. Even though the plant is a promising herb with various clinical applications in medicine and dentistry, more clinical research needs to be undertaken to validate and explain the action of acemannan in healing, so that it can be established in the field of medicine and a more precise understanding of the biological activities of these is required to develop Aloe vera as a pharmaceutical source.

  11. Salinity stress effects changed during Aloe vera L. vegetative growth

    Directory of Open Access Journals (Sweden)

    Jamal-Ali Olfati

    2012-05-01

    Full Text Available Salt stress is a limiting factor of plant growth and yield, and becoming a serious problem in the world so in reason to determine salinity effect on aloe growth experiment was conducted in a greenhouse as a bi-factorial in completely randomized experimental design with three replications. Aloe plants irrigated with nutrient solution containing different level of NaCl (0, 2, 4, 6 and 8 ms on December and harvesting took place three time with one month interval on January, February and March. Results revealed that salinity affected number of leaf, plant height, number of sprout, root weight, plant weight, leaf weight, total gel weight, and root dry weight. Variance analysis also showed that sampling time relieves significant effect on number of leaf, plant height, and root length, number of sprout, root weight, plant weight, leaf weight and total gel weight. All measured characteristics were highest for control. Interaction between salinity and sampling time on plant height, plant weight, leaf weight and total gel weight showed that these traits decreased in all salinity levels when sampled 30 days after transplanting and the lowest value was related to highest salinity. This result was similar 60 and 90 days after transplanting. Interaction between salinity and sampling time on root length showed that the highest root length was obtained in 2 and 4 ms salinity level when sampled 30 days after transplanting. However all salinity levels also decreased root length 90 days after transplanting.

  12. ANTIOXIDANT EFFECT OF ALOE VERA IN EXPERIMENTALLY INDUCED DIABETES MELLITUS

    Directory of Open Access Journals (Sweden)

    Jyoti Sethi et al

    2012-08-01

    Full Text Available The aim of the present study was to evaluate antidiabetic and antioxidant potential of Aloe vera (AV in alloxan induced diabetes in albino rabbits. Experimental Diabetes was induced in rabbits with alloxan(80mg/Kg body weightand animals showing fasting blood glucose levels more than 250mg/dl were considered as diabetics and divided into four groups of six each (n=6. Group I: Normal control rabbits, Group II: Alloxan induced diabetic rabbits, Group III: Diabetic rabbits received AV gel extract (300 mg/Kg in aqueous solution for 21 days, Group IV: diabetic rabbits given glibenclamide (600ug/kg in aqueous solution. All the drugs were administered orally (using an intra gastric tube in a single dose in the morning for 21 days. Blood samples were collected from the marginal vein of pinna of overnight fasted rabbits (Blood sugar, glycosylated hemoglobin (HbA1c, Malondialdehyde (MDA, reduced glutathione (GSH, total thiols (PSH and Superoxide dismutase (SOD. Oral administration of AV showed potent antihyperglycemic and anti-lipidperoxidative effect in diabetic animals. Simultaneously, the levels of protective antioxidant enzymes (SOD, GSH and PSH were significantly increased with AV supplementation. The results suggest potent antidiabetic and antioxidant potential of AV in experimental diabetes, and thus Aloe vera can be used as an alternative remedy for treatment of diabetes mellitus and its complications.

  13. The efficacy of aloe vera used for burn wound healing: a systematic review.

    Science.gov (United States)

    Maenthaisong, Ratree; Chaiyakunapruk, Nathorn; Niruntraporn, Surachet; Kongkaew, Chuenjid

    2007-09-01

    Aloe vera has been traditionally used for burn healing but clinical evidence remains unclear. We conducted a systematic review to determine the efficacy of topical aloe vera for the treatment of burn wounds. We electronically searched relevant studies in MEDLINE, CINAHL, Cochrane Library, HealthSTAR, DARE, South-East Asia Database, Chinese Databases, and several Thai local Databases (1918-June 2004). Only controlled clinical trials for burn healing were included. There were no restrictions on any language of publication. Two reviewers independently extracted data on study characteristics, patient characteristics, intervention, and outcome measure. Four studies with a total of 371 patients were included in this review. Based on a meta-analysis using duration of wound healing as an outcome measure, the summary weighted mean difference in healing time of the aloe vera group was 8.79 days shorter than those in the control group (P=0.006). Due to the differences of products and outcome measures, there is paucity to draw a specific conclusion regarding the effect of aloe vera for burn wound healing. However, cumulative evidence tends to support that aloe vera might be an effective interventions used in burn wound healing for first to second degree burns. Further, well-designed trials with sufficient details of the contents of aloe vera products should be carried out to determine the effectiveness of aloe vera.

  14. Effect of Aloe barbadensis Mill. formulation on Letrozole induced polycystic ovarian syndrome rat model

    Directory of Open Access Journals (Sweden)

    Radha Maharjan

    2010-01-01

    Full Text Available This is a preliminary study that explores the efficacy of Aloe vera gel formulation as a possible therapeutic agent in the prevention and management of polycystic ovary syndrome (PCOS. PCOS is recognized as the most common endocrinopathy of women. Increased androgen synthesis, disrupted folliculogenesis, and insulin resistance lie at the patho-physiological core of PCOS. Current therapy for such a syndrome is use of insulin sensitizers. Large randomized clinical trials of metformin as the insulin-sensitizing drug, however, suggested that it produces many side effects after prolonged usage. For this reason, an alternate therapy would be to use herbs with hypoglycemic potential. Aloe barbadensis Mill. (Liliaceae popularly known as Aloe vera is a well-known plant with such properties. The present study evaluated the efficacy of Aloe vera gel formulation in a PCOS rat model. Five month old Charles Foster female rats were orally fed with letrozole, a non-steroidal aromatase inhibitor, to induce PCOS. The rats were then treated orally with the Aloe vera gel formulation (1 ml dose daily for 45 days. This restored their estrus cyclicity, glucose sensitivity, and steroidogenic activity. Co-treatment of the inductive agent (letrozole with the Aloe vera gel prevented the development of the PCO phenotype. Aloe vera gel formulation exerts a protective effect in against the PCOS phenotype by restoring the ovarian steroid status, and altering key steroidogenic activity. This can be attributed to phyto-components present in the extract.

  15. Quality change of apple slices coated with Aloe vera gel during storage.

    Science.gov (United States)

    Song, Hye-Yeon; Jo, Wan-Shin; Song, Nak-Bum; Min, Sea C; Song, Kyung Bin

    2013-06-01

    Fresh-cut apples are easily susceptible to browning and microbial spoilage. In this study, an edible coating prepared from Aloe vera gel containing antibrowning solution was applied to preserve the quality of fresh-cut apples during storage. Fresh-cut apples were treated with both an Aloe vera gel and an Aloe vera gel containing 0.5% cysteine and then stored at 4 °C for 16 d. The color, firmness, weight loss, soluble solid content, titratable acidity, microbial analysis, and sensory evaluation were analyzed during storage. Fresh-cut apples coated with the Aloe vera gel showed delayed browning and reduced weight loss and softening compared to the control. The Aloe vera gel coating was also effective in reducing the populations of the total aerobic bacteria and yeast and molds. In particular, Aloe vera gel containing 0.5% cysteine was most effective in delaying browning and the reduction of microbial populations among the treatments. These results suggest that an Aloe vera gel coating can be used for maintaining the quality of fresh-cut apples.

  16. Protective effects of Aloe vera-based diets in Eimeria maxima-infected broiler chickens.

    Science.gov (United States)

    Yim, Dongjean; Kang, Sang S; Kim, Dong W; Kim, Sang H; Lillehoj, Hyun S; Min, Wongi

    2011-01-01

    Aloes have been widely used for a broad range of pharmacological activities, including parasitic problems. Avian coccidiosis is the most costly and wide-spread parasitic disease in the poultry industry, and has been mainly controlled by the use of chemotherapeutic agents. Due to the emergence of drug-resistant strains, alternative control strategies are needed. In this study, the protective effects of Aloe vera-based diets were assessed in broiler chickens following oral infection with Eimeria maxima. Chickens were fed a regular diet supplemented with ground Aloe vera throughout the duration of the experiment beginning 2 days prior to infection with 1 × 10(4) sporulated oocysts of E. maxima. No significant differences were found in body weight gain or loss between the Aloe vera-supplemented and unsupplemented groups with or without E. maxima infections. Fecal oocyst shedding decreased significantly (p Aloe vera as compared to the unsupplemented group. Furthermore, the Aloe vera-supplemented group showed significantly fewer intestinal lesions (p Aloe vera could be used an alternative treatment for controlling avian coccidiosis.

  17. Study on the adsorptive catalytic voltammetry of aloe-emodin at a carbon paste electrode

    Institute of Scientific and Technical Information of China (English)

    LI; Ju'nan; GAO; Peng; LI; Xiangling; YAN; Zhihong; MAO; Xu

    2005-01-01

    A new catalytic voltammetric method for the determination of anthraqunone medicines at a carbon paste electrode (CPE) was described for the first time. The mechanism of the catalytic reaction was investigated by using linear sweep voltammetry, cyclic voltammetry, constant potential electrolysis and so on. The experiment results indicate that aloe-emodin was efficiently accumulated at a CPE by adsorption. In the following potential scan, aloe-emodin was reduced to homologous anthrahydroquinone compound, then the compound was immediately oxidized to aloe-emodin by the dissolved oxygen, and the aloe-emodin was again reduced at the CPE. As a result, a cyclic catalytic reaction was established. But a reversible redox reaction of aloe-emodin can only be observed at a mercury electrode, no catalytic reaction occurs there. A sensitive catalytic voltammetric peak of aloe-emodin was obtained at about -0.60 V (vs. SCE) in 0.56 mol/L NH3-NH4Cl buffer (pH 8.9). The proposed method was applied to the determination of aloe-emodin in the Radix Rhei with satisfactory results. The determination results were in good agreement with reference values obtained by the HPLC. The adsorptive catalytic voltammetry for the determination of organic compound at CPE, chemically modified electrode and other solid electrodes could be significant in the studies on pharmacology, pharmacodynamics, toxicity of medicine, clinical medicine and biochemistry.

  18. Variabilidad de aislados diazotróficos simbióticos en diferentes condiciones agroecológicas del sur del Ecuador

    OpenAIRE

    Torres Gutiérrez, Roldán

    2014-01-01

    The study consistedin determiningthe variability ofsymbioticdiazotrophics isolatesfrom common bean(Phaseolus vulgarisL.) in several agro-ecological zonesof Loja province, Ecuador. We sampleda total of 9cantonswith differentagro-climatic zones, which were geo-referenced using theGlobalPositioning System. Thevariabilityof the isolates was determined by morphological characterization ofthe colonies,where Gramstain, growth, color, mucus production, edges and elevation of the colonies were evaluat...

  19. Agentes bacterianos asociados a brotes de Enfermedades Trasmitidas por Alimentos (ETA) aislados de coprocultivos - Bacterial agents associated to foodborne outbreaks isolated from foods and faecal samples

    OpenAIRE

    Sedrés Cabrera, Martha; Barreto Argilagos, Guillermo; Guevara Viera, Guillermo; Rodríguez Torrens, Herlinda

    2010-01-01

    ResumenEl trabajo aborda la incidencia de agentes bacterianos, aislados decoprocultivos realizados a pacientes con diarreas agudas. Todos involucrados en 72 brotes de enfermedades transmitidas por alimentos (ETA) estudiados por el Centro Provincial de Higiene, Epidemiología y Microbiología “Mártires de Pino Tres” de la ciudad de Camagüey. A tal fin se procesó la información recogida en su Sección de Coprocultivos.Este estudio generó un total de 722 muestras de las cuales 279 (38,64%)posibilit...

  20. Ação do anticonvulsivante isolado e associado ao midazolam como medicação pré-anestésica sobre o índice bispectral (BIS em pacientes com paralisa cerebral Acción del antiepiléptico aislado y asociado al midazolam como medicación preanestésica sobre el índice bispectral (BIS en pacientes con parálisis cerebral Effect of isolated anticonvulsant drug use and associated to midazolam as pre-anesthetic medication on the bispectral index (BIS in patients with cerebral palsy

    Directory of Open Access Journals (Sweden)

    Verônica Vieira da Costa

    2010-06-01

    ás utilizado como medicación preanestésica y no se conocen sus interacciones medicamentosas en los pacientes con PC. El objetivo de este estudio fue evaluar el midazolam como medicación preanestésica en el BIS de los pacientes con PC en uso crónico de antiepilépticos. MÉTODO: Se evaluaron tres grupos de pacientes: PC sin uso de antiepilépticos, PC en uso de antiepiléptico y otro grupo sin enfermedad y sin uso de medicaciones (grupo control. En la víspera de la cirugía, con los pacientes despiertos y en decúbito dorsal, fue colocado el monitor del BIS y se registraron los valores basales del BIS. Al día siguiente, 40 minutos antes de la cirugía, los pacientes recibieron 0,6 mg.kg-1 de midazolam por vía oral. Antes del inicio de la anestesia fue realizado el mismo procedimiento para registro del BIS, después del uso del midazolam. RESULTADOS: Fueron estudiados 107 pacientes, 39 pacientes del grupo control y 68 con diagnóstico de PC. De ellos, 17 usaban antiepilépticos. Con relación al valor promedio de BIS después del uso del midazolam, no hubo diferencia entres los pacientes del grupo control y del grupo PC que no tomaban antiepiléptico, mientras que los pacientes que usaban antiepilépticos fueron diferentes (p = 0,003. La posibilidad de disminución del BIS después del uso del midazolam, aumenta de acuerdo con el número de antiepiléptico usado por el paciente. CONCLUSIONES: El uso crónico de antiepiléptico asociado al midazolam vía oral como medicación preanestésica, puede conllevar a la disminución de los valores de BIS configurando niveles profundos de hipnosis.BACKGROUND AND OBJECTIVES: Patients with cerebral palsy (CP frequently receive drugs for the treatment of concomitant diseases, such as seizures. Midazolam is a benzodiazepine with hypnotic action most often used as pre-anesthetic medication and its drug interactions in patients with CP are unknown. The objective of the present study was to evaluate the effect of midazolam as pre

  1. Inkassofirma ähvardab Alo Streimanni kaatrifirmat kohtuga / Andres Kärssin

    Index Scriptorium Estoniae

    Kärssin, Andres, 1971-

    2005-01-01

    Eesti Posti eksjuhile Alo Streimannile kuuluv kaatritehas ScandiNaval ja temaga seotud ehitusfirma OÜ TRK Ehitus on võlgades. Vt. samas: Gea Velthut-Sokka. Võlad on ka Streimanniga seotud ehitusfirmal

  2. Effects of highly purified anthraquinoid compounds from Aloe vera on sensitive and multidrug resistant leukemia cells.

    Science.gov (United States)

    Grimaudo, S; Tolomeo, M; Gancitano, R; Dalessandro, N; Aiello, E

    1997-01-01

    Folk medicine has attributed antitumor properties to preparations from Aloe vera. We have studied the effects of five purified compounds from the plant on human K562 leukemia and on its multidrug resistant (MDR) variant, K562/R. The glycosides aloin A and B, aloesin and aloeresin were devoid of antitumor activity up to 200 mu M concentrations. Only the aglycone aloe emodin produced reproducible antitumor effects, which, interestingly, were more pronounced in the MDR, P-glycoprotein overexpressing, cell line. Its IC50 was in fact 29 mu M in K562 and 10.5 mu M in K562/R. Aloe emodine caused mainly cytostasis and accumulation of the cells in the S and G(2)-M phases of the cell cycle during the first 48 h of treatment. Thereafter, massive cell death ensued. Research on the antitumor activity of compounds extracted from Aloe vera probably deserves continuation.

  3. An investigation of the potential application of chitosan/aloe-based membranes for regenerative medicine.

    Science.gov (United States)

    Silva, S S; Popa, E G; Gomes, M E; Cerqueira, M; Marques, A P; Caridade, S G; Teixeira, P; Sousa, C; Mano, J F; Reis, R L

    2013-06-01

    A significant number of therapeutics derived from natural polymers and plants have been developed to replace or to be used in conjunction with existing dressing products. The use of the therapeutic properties of aloe vera could be very useful in the creation of active wound dressing materials. The present work was undertaken to examine issues concerning structural features, topography, enzymatic degradation behavior, antibacterial activity and cellular response of chitosan/aloe vera-based membranes. The chitosan/aloe vera-based membranes that were developed displayed satisfactory degradation, roughness, wettability and mechanical properties. A higher antibacterial potency was displayed by the blended membranes. Moreover, in vitro assays demonstrated that these blended membranes have good cell compatibility with primary human dermal fibroblasts. The chitosan/aloe vera-based membranes might be promising wound dressing materials.

  4. The combined effects of Aloe vera gel and silver nanoparticles on wound healing in rats

    Directory of Open Access Journals (Sweden)

    Y. Yousefpoor

    2016-01-01

    Full Text Available Objective(s: This study was aimed at investigating the synergy effects of Aloe vera gel and silver nanoparticles on the healing rate of the cutting wounds. Materials and Methods: In order to determine the concentration of silver nanoparticles in Aloe vera gel, the MBC methods were applied on the most common bacteria infecting wounds, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. The cutting wounds with Full-thickness skin were dorsally created on rats; then the rats were divided into 4 groups. The treatments groups included: mixture of Aloe vera gel and silver nanoparticles, Aloe vera gel alone and silver nanoparticles alone in addition to control groups. The treatment was carried out for 2 weeks and the size of the wound closures were measured by an image software analysis. Results:There was no significant difference (p

  5. Aloe vera: a valuable ingredient for the food, pharmaceutical and cosmetic industries--a review.

    Science.gov (United States)

    Eshun, Kojo; He, Qian

    2004-01-01

    Scientific investigations on Aloe vera have gained more attention over the last several decades due to its reputable medicinal properties. Some publications have appeared in reputable Scientific Journals that have made appreciable contributions to the discovery of the functions and utilizations of Aloe--"nature's gift." Chemical analysis reveals that Aloe vera contains various carbohydrate polymers, notably glucomannans, along with a range of other organic and inorganic components. Although many physiological properties of Aloe vera have been described, it still remains uncertain as to which of the component(s) is responsible for these physiological properties. Further research needs to be done to unravel the myth surrounding the biological activities and the functional properties of A. vera. Appropriate processing techniques should be employed during the stabilization of the gel in order to affect and extend its field of utilization.

  6. Comparative efficacy of aloe vera mouthwash and chlorhexidine on periodontal health: A randomized controlled trial

    Science.gov (United States)

    Jha, Abhishek; Bhashyam, Mamtha

    2016-01-01

    Background With introduction of many herbal medicines, dentistry has recently evidenced shift of approach for treating many inflammatory oral diseases by using such modalities. Aloe vera is one such product exhibiting multiple benefits and has gained considerable importance in clinical research recently. Aim To compare the efficacy of Aloevera and Chlorhexidine mouthwash on Periodontal Health. Material and Methods Thirty days randomized controlled trial was conducted among 390 dental students. The students were randomized into two intervention groups namely Aloe Vera (AV) chlorhexidine group (CHX) and one control (placebo) group. Plaque index and gingival index was recorded for each participant at baseline, 15 days and 30 days. The findings were than statistically analyzed, ANOVA and Post Hoc test were used. Results There was significant reduction (pAloe Vera (AV) and chlorhexidine group. Post hoc test showed significant difference (paloe Vera and placebo and chlorhexidine and placebo group. No significant difference (pAloe vera, chlorhexidine, dental plaque, gingivitis. PMID:27703614

  7. GROSS TOXICITIES AND HEPATOPROTECTIVE EFFECT OF ALOE VERA (L BURM.F

    Directory of Open Access Journals (Sweden)

    Nuzhat Sultana

    2012-10-01

    Full Text Available Traditional herbal drugs have a great demand in under developed countries due to their efficacy, low cost and lesser adverse effects, and they are considered to be “natural. Aloe vera at the dose of 500 mg body weight per oral was studied for the gross toxicities and hepatoprotective effect and observed the level of liver biochemical parameters in rabbits. Aloe vera showed highly significant (p<0.001 hepatoprotective effect by lowering the serum levels of serum glutamic oxaloacetate transaminase (SGOT, serum glutamic pyruvates transaminase (SGPT and direct bilirubin. The overall experimental results suggests that Aloe vera protects the liver from oxidative stress and inhibits the excessive free radicals accumulation and possessing many hepatoprotective phytoconstituents which are biologically active such as flavonoids, alkaloids, they may be responsible for the significant hepatoprotective activity and the results justify the use of Aloe vera as a hepatoprotective agent.

  8. A revised generic classification for Aloe (Xanthorrhoeaceae subfam. Asphodeloideae)

    DEFF Research Database (Denmark)

    Grace, Olwen Megan; Klopper, Ronell R.; Smith, Gideon F.;

    2013-01-01

    The predominantly southern African Xanthorrhoeaceae subfam. Asphodeloideae Asphodelaceae subfam. Alooideae) has long been regarded as comprising seven so-called alooid genera (Aloe, Astroloba, Chortolirion, Gasteria, Haworthia, Lomatophyllum, Poellnitzia). A reassessment of the classification...

  9. Interaction of aloe-emodin with human serum albumin

    Institute of Scientific and Technical Information of China (English)

    DU JinFeng; LI Ying; ZHANG Qi; YAO XiaoJun

    2007-01-01

    The presence of several high affinity binding sites on human serum albumin (HAS) makes it a possible target for many drugs. This study is designed to examine the effect of aloe-emodin on HAS by fluorescence, CD spectroscopy and molecular modeling. The results of fluorescence measurements suggested that the hydrophobic interaction was the predominant intermolecular force stabilizing the AE-HAS complex, which was in good agreement with the result of molecular modeling study. And the enthalpy change ΔH0 and the entropy change ΔS0 were calculated to be -7.041 kJ·mol-1 and 76.619 J·mol-1·K-1 according to the Van't Hoff equation. The alterations of protein secondary structure in the presence of AE in aqueous solution were quantitatively calculated from CD spectra, and the content of α-helices obviously increased.

  10. A review on ethnopharmacological potential of Aloe vera L.

    Directory of Open Access Journals (Sweden)

    Md. Shamim Hossain

    2013-04-01

    Full Text Available In recent years, Aloe vera Linn. (Ghritokumari locally has become a subject of interest because of its beneficial effects on human health. The present ethnopharmacological review was conducted to evaluate the therapeutic properties of A. vera by scientific evidences. It belongs to the family Liliaceae, is a perennial herb with 30-60cm long juicy leaves which is found all over Bangladesh. To date, more than 75 active ingredients including aloesin, aloeemodin, acemannan, aloeride, methylchromones, flavonoids, saponin, amino acids, vitamins, and minerals have been identified from inner gel of leaves. It has antiinflammatory, antioxidant, antimicrobial, anticancer, antidiabetic, immuneboosting, and hypoglycemic properties. Daily supplementation with this is effective against stroke, heart attacks, leukemia, anemia, hypertension, AIDS, radiation burns, digestive disorders etc. This study also covers its taxonomy, distribution, morphology, and monograph. [J Intercult Ethnopharmacol 2013; 2(2.000: 113-120

  11. Tyrosinase inhibitory components from Aloe vera and their antiviral activity.

    Science.gov (United States)

    Kim, Jang Hoon; Yoon, Ju-Yeon; Yang, Seo Young; Choi, Seung-Kook; Kwon, Sun Jung; Cho, In Sook; Jeong, Min Hee; Ho Kim, Young; Choi, Gug Seoun

    2017-12-01

    A new compound, 9-dihydroxyl-2'-O-(Z)-cinnamoyl-7-methoxy-aloesin (1), and eight known compounds (2-9) were isolated from Aloe vera. Their structures were elucidated using 1D/2D nuclear magnetic resonance and mass spectra. Compound 9 exhibited reversible competitive inhibitory activity against the enzyme tyrosinase, with an IC50 value of 9.8 ± 0.9 µM. A molecular simulation revealed that compound 9 interacts via hydrogen bonding with residues His244, Thr261, and Val283 of tyrosinase. Additionally, compounds 3 and 7 were shown by half-leaf assays to exhibit inhibitory activity towards Pepper mild mottle virus.

  12. Electrical signaling in Aloe vera induced by localized thermal stress.

    Science.gov (United States)

    Volkov, Alexander G; Lang, Ryan D; Volkova-Gugeshashvili, Maia I

    2007-11-01

    Action potentials in higher plants are theorized as the information carriers in intercellular and intracellular communication in the presence of environmental stressors. Among the most common stressors is heat shock. Under stressful conditions, the response reactions of plant tissues and organs can be local or transmitted over long distances. In this article, the speeds of propagation of thermally induced action potentials in green plants are discussed, and their speeds were found to be comparable to those occurring in various mammalian species. These rapid action potentials in green plants were recorded in real time using modern data acquisition techniques. According to our measurements, a single application of localized heat stress induces fast action potentials in Aloe vera (67 m/s). Electrical signals propagated along all leaves of the A. vera plants were studied. Possible pathways for electrical signal propagation in vascular plants are also discussed.

  13. Cytotoxic Quinones from the Roots of Aloe dawei

    Directory of Open Access Journals (Sweden)

    Negera Abdissa

    2014-03-01

    Full Text Available Seven naphthoquinones and nine anthraquinones were isolated from the roots of Aloe dawei by chromatographic separation. The purified metabolites were identified by NMR and MS analyses. Out of the sixteen quinones, 6-hydroxy-3,5-dimethoxy-2-methyl-1,4-naphthoquinone is a new compound. Two of the isolates, 5,8-dihydroxy-3-methoxy-2-methylnaphthalene-1,4-dione and 1-hydroxy-8-methoxy-3-methylanthraquinone showed high cytotoxic activity (IC50 1.15 and 4.85 µM on MCF-7 breast cancer cells, whereas the others showed moderate to low cytotoxic activity against MDA-MB-231 (ER Negative and MCF-7 (ER Positive cancer cells.

  14. An investigation of the potential application of chitosan/aloe-based membranes for regenerative medicine

    OpenAIRE

    SILVA, S. S.; Popa, Elena Geta; Gomes, Manuela E.; Cerqueira, M. T.; MARQUES, A.P.; Caridade, S. G.; Teixeira, P; Sousa,Cláudia; Mano, J. F.; Reis, R. L.

    2013-01-01

    A significant number of therapeutics derived from natural polymers and plants have been developed to replace or to be used in conjunction with existing dressing products. The use of the therapeutic properties of aloe vera could be very useful in the creation of active wound dressing materials. The present work was undertaken to examine issues concerning structural features, topography, enzymatic degradation behavior, antibacterial activity and cellular response of chitosan/aloe ve...

  15. Strong enhancement of antioxidant activity of Aloe vera extracts by gamma irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eun Mi; Bai, Hyoung Woo; Lee, Seung Sik; Hong, Sung Hyun [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Cho, Jae Young; Chung, Byung Yeoup [Chonbuk National University, Jeonju (Korea, Republic of)

    2011-10-15

    The World Health Organization (WHO) has estimated that approximately 80% of individuals rely on traditional medicines. Among over 400 Aloe species, Aloe vera was the most accepted species for various medical, cosmetic and neutraceutical purposes. Aloe vera (syn.: Aloe barbadensis Miller) was a perennial succulent plant belonging to the Aloeaceae family (subfamily of the Asphodelaceae). It has been reported that Aloe vera extracts were useful in the treatment of wound and burn healing, minor skin infections, sebaceous cyst, diabetes, and elevated blood lipids in humans. Recent studies have shown that treatment with either Aloe vera crude gel or its extracts, such as acemannan, {beta}-sitosterol, and others, resulted in faster healing of wounds by stimulating fibroblast proliferation, collagen deposition, angiogenesis, and production of growth factors. Ionizing radiation technology has been developed to improve our daily life such as cancer therapy and sterilizing tool due to its unique feature that could be penetrated biomaterials leading to alter their own physical properties. More recently, many studies have attempted to apply the radiation technology to enhance their biological activities. At present, however, very little was known about whether naturally-occurring phenolic compounds of ethanolic aloe gel extracts that were altered their biological activities by ionizing radiation to serve as antioxidant in the body to reduce ROS produced by the stresses. The purpose of the current study was to investigate the influence of gamma irradiation on antioxidant activity of Aloe vera extracts, and open insight new possibilities that gamma ray could be a powerful tool for improving its own biological activities

  16. TSS mapping using ALOS and THEOS imageries over Penang island

    Science.gov (United States)

    Sim, C. K.; Lim, H. S.; MatJafri, M. Z.; Abdullah, K.

    2013-05-01

    Environmental monitoring through the method of traditional ship sampling is time consuming and requires a high survey cost. This study uses an empirical model, based on actual water quality of total suspended solids (TSS) measurements from the Penang Strait, Malaysia to predict TSS based on optical properties of satellite digital imagery using advanced land observing satellite imagery (ALOS) and Thai earth observation system (THEOS) satellite imageries. The proposed algorithm is based on the reflectance model that is a function of the inherent optical properties of water, which can be related to its constituent's concentrations. Water samples were collected simultaneously with the airborne image acquisition and later analyzed in the laboratory. Water sample's locations were determined by using a handheld GPS. The digital numbers for each band corresponding to the sea-truth locations were extracted and then converted into radiance values and reflectance values. The reflectance values were used for calibration of the water quality algorithm. The efficiency of the proposed algorithm was investigated based on the observations of correlation coefficient (R) and root-mean-square deviations (RMS) with the sea-truth data. This algorithm was then used to map the TSS concentration over Penang, Malaysia. The TSS map was color-coded and geometrically corrected for visual interpretation. The calibrated algorithm had the R and RMS values of 0.9012 and 5.3651 mg/l respectively for ALOS data. The R and RMS values were 0.8515 and 6.2635 mg/l respectively for THEOS data. This study indicates that TSS mapping can be carried out using remote sensing technique of the satellite digital photography system over Penang, Malaysia.

  17. SYNTHESIS AND CHARACTERIZATION OF BIODEGRDABLE PLASTIC FROM CASAVA STARCH AND ALOE VERA EXTRACT WITH GLYCEROL PLASTICIZER

    Directory of Open Access Journals (Sweden)

    Mery Apriyani

    2016-05-01

    Full Text Available Synthesis and characterizations of Biodegradable Plastic made of Cassava Waste Starch, glycerol, acetic acid and Aloe vera extract has done. The aims of this research are to study the influence of addition of aloe vera extract in plastics mechanics properties, water vapor transmission rate and biodegradation. There are five main steps in this research, extraction of aloe vera, cassava starch preparation from cassava waste, preparations, characterization and biodegradability study of biodegradable plastic. The addition variations of aloe vera extract that used in this research are 0.01; 0.03; 0.05; 0.07 and 0.14 grams. Results showed that the addition of aloe Vera tends to increased biodegrable plastic thickness to 0.01 mm and elongation to 32.07%. However, biodegradable plastic tensile strength tends to decreased to 23.95 Mpa. Optimum tensile strength is 3.90 Mpa and elongation is 34.43%. Optimum water vapor transmission rate is 2.40 g/m2hours. Biodegradation study of biodegradable plastic showed that addition of aloe vera extract doesn’t significantly influence in plastic degradations.

  18. Hepatotherapeutic effect of Aloe vera in alcohol-induced hepatic damage.

    Science.gov (United States)

    Saka, W A; Akhigbe, R E; Ishola, O S; Ashamu, E A; Olayemi, O T; Adeleke, G E

    2011-07-15

    There is a lack of reliable hepatotherapeutic drugs in modern medicine in the management of alcohol/drug-induced liver damage. Aloe vera extract has been used in folklore medicine for its medicinal values. This study evaluates the hepatotherapeutic activity of aqueous extract of Aloe vera gel in rats. Sprague-Dawley rats were divided into three groups; the negative control, positive control and the extract-treated groups. The negative control received only distilled water daily. The positive control received alcohol, while the extract-treated group received aqueous extract of Aloe vera and alcohol. Hepatotoxicity was induced in the positive control and extract-treated rats with alcohol. The hepatotherapeutic effect was evaluated by performing an assay of the serum total bilirubin, alkaline phosphatase, aspartate and alanine transaminases and liver histopathology. Alanine transaminase activities were comparable in all groups. Alcohol treatment alone significantly (p Aloe vera extract. Histopathological examination revealed that alcohol induced hepatic damage. Aloe vera treatment maintained hepatic architecture similar to that seen in the control. This study shows that aqueous extract of Aloe vera gel is hepatotherapeutic and thus lends credence to the use of the plant in folklore medicine in the management of alcohol-induced hepatic dysfunction.

  19. [Bacteriostatic and/or bactericidal extract of Aloe vera gel on cultures of Listeria monocytogenes].

    Science.gov (United States)

    Ramírez Mérida, Luis Guillermo; Morón de Salim, Alba; Catinella, Rosangela; Castillo, Luis

    2012-03-01

    Listeria monocytogenes is a bacteria responsible for food borne diseases (FBD). The effect of Aloe vera gel extract as a possible bacteriostatic and/or bactericidal against Listeria monocytogenes, was checked by determined the minimum inhibitory concentration (MIC), the time of minimum inhibition (TMI) and minimum bactericidal concentration (MBC) solutions extract of Aloe vera gel in different concentrations on cultures of Listeria monocytogenes ATCC 7635. We applied the agar diffusion method, using solutions of extract of Aloe vera gel at concentrations of 0 to 100% for the MIC. The TMI was determined by growth curves in trypticase soy broth with an initial inoculum of Listeria monocytogenes ATCC 7635 of 108 CFU/mL in each solution. It was determined that the MIC was 10% extract of Aloe vera gel and TMI was 5 hours at concentrations of 10%, 20% and 30% of Aloe vera, while concentrations of 50, 80, 90 and 100%, the time was 8 hours. It was found that indeed the Aloe vera gel is bacteriostatic power on Listeria monocytogenes (p < 0.001), but yet, no bactericidal effect was obtained in our study.

  20. Aloe vera and Vitis vinifera improve wound healing in an in vivo rat burn wound model.

    Science.gov (United States)

    Lin, Li-Xin; Wang, Peng; Wang, Yu-Ting; Huang, Yong; Jiang, Lei; Wang, Xue-Ming

    2016-02-01

    Aloe vera and Vitis vinifera have been traditionally used as wound healing agents. The present study aimed to investigate the effects of aloe emodin and resveratrol in the burn wound healing procedure. Burn wounds are common in developed and developing countries, however, in developing countries, the incidence of severe complications is higher and financial resources are limited. The results of the present study demonstrated that neither aloe emodin or resveratrol were cytotoxic to THP-1 macrophages at concentrations of 1, 100 and 500 ng/ml. A significant increase in wound-healing activity was observed in mice treated with the aloe emodin and resveratrol, compared with those which received control treatments. The levels of IL-1β in the exudates of the burn wound area of the treated mice increased in a time-dependent manner over 7 days following burn wound injury. At 10 days post-injury, steady and progressive wound healing was observed in the control animals. The present study confirmed that increased wound healing occurs following treatment with aloe emodin,, compared with resveratrol, providing support for the use of Aloe vera plants to improve burn wound healing.

  1. Herencias culturales desconocidas, el caso del patrimonio industrial mexicano

    OpenAIRE

    Victoria Novelo Oppenheim

    2005-01-01

    Si bien la noción de "patrimonio cultural" se ha transformado a lo largo de la historia reciente de México, éste se refiere fundamentalmente al aprecio y conservación de los monumentos y sitios de importancia en la vida prehispánica y colonial del país. A pesar de algunos esfuerzos aislados, no se ha ampliado para incluir la preservación y difusión de las herencias que dejaron las primeras etapas de formación de un sistema de fábricas, tanto en forma física como intangible. La autora argument...

  2. Effects of drying temperature and ethanol concentration on bipolar switching characteristics of natural Aloe vera-based memory devices.

    Science.gov (United States)

    Lim, Zhe Xi; Cheong, Kuan Yew

    2015-10-28

    Extracted, formulated, and processed natural Aloe vera has been used as an active layer for memory applications. The functional memory device is realized by a bottom-up structure of ITO/Aloe vera/Al in which the Aloe vera is spin-coated after mixing with different concentrations of ethanol (0-80 wt%) and subsequently dried at different temperatures (50-120 °C). From the current density-voltage measurements, the device can exhibit a reproducible bipolar switching characteristic with pure Aloe vera dried at 50 °C. It is proposed that charges are transported across the Aloe vera layer via space-charge-limited conduction (SCLC), and clusters of interstitial space formed by the functional groups of acemannans and de-esterified pectins in the dried Aloe vera contribute to the memory effect. The formation of charge traps in the Aloe vera layer is dependent on the drying temperature. The drying temperature of a memory-switching Aloe vera layer can be extended to 120 °C with the addition of appropriate amounts of ethanol. The concept of using natural Aloe vera as an active material for memory applications has been demonstrated, and the read memory window, ON/OFF ratio, and retention time are approximately 5.0 V, 10(3), and >10(4) s, respectively.

  3. IDENTIFICATION OF PHYTO-COMPONENTS AND ITS BIOLOGICAL ACTIVITIES OF ALOE VERA THROUGH THE GAS CHROMATOGRAPHY-MASS SPECTROMETRY

    Directory of Open Access Journals (Sweden)

    P.T.V Lakshmi

    2011-05-01

    Full Text Available In this study, the bioactive components of Aloe vera leaves have been evaluated using GC/MS. The chemical compositions of the n-hexane extract of Aloe vera were investigated using Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST library. GC/MS analysis of n-hexane extract of Aloe vera revealed the existence of twenty six bioactive compounds. The results of this study offer a platform of using Aloe vera as herbal drug for cancer studies.

  4. Stimulation of osteoblast activity by induction of Aloe vera and xenograft combination

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2011-12-01

    Full Text Available Background: Tooth extraction is generally followed by alveolar ridge resorption that later can cause flat ridge. Aloe vera have biogenic stimulator and hormone activities for wound healing. Purpose: This study was aimed to know osteoblast activities in alveolar bone after induction of Aloe vera and XCB combination. Methods: Fifty four of Cavia cabaya were divided into three main groups. Group I was control group. Group II was filled with xenograft concelous bovine (XCB and group III was filled with the combination of Aloe vera gel and XCB. Then, each group was divided into three sub groups according to timing, they are 14, 30, and 60 days after tooth extraction and application. Histology and morphology examination were performed on the harvested specimens. Results: There were significant differences between the control group and the other groups filled with the combination of Aloe vera and XCB. Conclusion: In conclusion, the application of Aloe vera gel and xenograft combination decrease the number of osteoclast and increase the number of osteoblast in post tooth extraction alveolar bone structure indicating the new growth of alveolar bone.Latar belakang: Pencabutan gigi pada umumnya selalu diikuti resopsi tulang alveolar, sehingga bila terjadi dalam waktu yang lama ridge akan menjadi flat. Aloe vera adalah bahan stimulasi biogenik dan mempunyai aktivitas hormon untuk proses penyembuhan luka. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui aktivitas osteoblas pada tulang alveol dengan pemberian kombinasi Aloe vera gel dan xenograft concelous bovine (XCB. Metode: Lima puluh empat ekor Cavia cabaya, dibagi menjadi 3 kelompok besar, kelompok pertama adalah kelompok kontrol yaitu hanya dilakukan pencabutan saja tanpa perlakuan, kelompok ke-2 yaitu kelompok yang setelah dicabut diberi XCB saja dan kelompok ke-3 yaitu kelompok yang setelah pencabutan diberi kombinasi Aloe vera gel dengan XCB pada luka bekas pencabutan gigi. Kemudian masing

  5. In vivo safety evaluation of UP780, a standardized composition of aloe chromone aloesin formulated with an Aloe vera inner leaf fillet.

    Science.gov (United States)

    Yimam, Mesfin; Brownell, Lidia; Jia, Qi

    2014-08-01

    Safety profiles of the aloe chromone aloesin or Aloe vera inner leaf fillet (Qmatrix) as a well tolerated entity have been reported separately. UP780, a standardized composition of aloe chromone formulated with an Aloe vera inner leaf fillet, has shown a significant beneficial effect in lowering blood glucose and improving insulin resistance in human. Here we evaluate the safety of UP780 after a repeated 14 and 90-day oral administration in CD-1 mice. UP780 was given at doses of 100mg/kg/day, 500mg/kg/day and 1000mg/kg/day to groups of 10 male and 10 female for 90days or administered by oral gavage at a dose of 2g/kg/day to groups of 5 male and 5 female for 14days. Body weight, feed consumption, hematology, clinical chemistry and histopathologic evaluation were performed. UP780 at a dose of 1000mg/kg/day or at 2000mg/kg/day produced no treatment-related toxicity or mortality. Body weight gain or feed consumption was similar between groups. There was no test article-related microscopic change. Spontaneously occurring minor changes in clinical chemistry and hematology were observed. However, these changes were limited to one sex or were not dose correlated. UP780 was well tolerated in this strain. A dose of 2000mg/kg/day was identified as the NOAEL (no-observed-adverse-effect-level).

  6. A comparison of the leaf gel extracts of Aloe ferox and Aloe vera in the topical treatment of atopic dermatitis in Balb/c mice.

    Science.gov (United States)

    Finberg, M J; Muntingh, G L; van Rensburg, C E J

    2015-12-01

    Aloe vera gel is widely used in the treatment of an array of disturbances, especially skin disorders. The wound-healing effects have been attributed to its moisturizing and anti-inflammatory effects as well as its beneficial effect on the maturation of collagen. The aim of the present study is to compare the effects of topically applied extracts of Aloe ferox with that of Aloe vera on the symptoms as well as IgE levels of a mouse model of atopic dermatitis (AD). Mice were sensitized and challenged with 2,4-dinitrochlorobenzene and treated afterwards for 10 consecutive days with the gels of either A. ferox or A. vera applied topically to the affected areas. A placebo gel was used for the control mice. Blood was collected at the beginning and end of the treatment period to measure serum IgE levels. Although the gels of both the Aloe species inhibited the cutaneous inflammatory response as well as serum IgE levels in the rats, the extracts of A. ferox were superior to that of A. vera in reducing IgE levels. The gels of A. ferox and A. vera, applied topically, may be a safe and useful alternative to antihistamines and topical corticosteroids, for the treatment of patients suffering from recurring chronic AD.

  7. Clear evidence of carcinogenic activity by a whole-leaf extract of Aloe barbadensis miller (aloe vera) in F344/N rats.

    Science.gov (United States)

    Boudreau, Mary D; Mellick, Paul W; Olson, Greg R; Felton, Robert P; Thorn, Brett T; Beland, Frederick A

    2013-01-01

    Aloe barbadensis Miller (Aloe vera) is an herbal remedy promoted to treat a variety of illnesses; however, only limited data are available on the safety of this dietary supplement. Drinking water exposure of F344/N rats and B6C3F1 mice to an Aloe vera whole-leaf extract (1, 2, and 3%) for 13 weeks resulted in goblet cell hyperplasia of the large intestine in both species. Based upon this observation, 2-year drinking water studies were conducted to assess the carcinogenic potential of an Aloe vera whole-leaf extract when administered to F344/N rats (48 per sex per group) at 0.5, 1, and 1.5%, and B6C3F1 mice (48 per sex per group) at 1, 2, and 3%. Compared with controls, survival was decreased in the 1.5% dose group of female rats. Treatment-related neoplasms and nonneoplastic lesions in both species were confined primarily to the large intestine. Incidences of adenomas and/or carcinomas of the ileo-cecal and cecal-colic junction, cecum, and ascending and transverse colon were significantly higher than controls in male and female rats in the 1 and 1.5% dose groups. There were no neoplasms of the large intestine in mice or in the 0 or 0.5% dose groups of rats. Increased incidences of mucosa hyperplasia of the large intestine were observed in F344/N rats, and increased incidences of goblet cell hyperplasia of the large intestine occurred in B6C3F1 mice. These results indicate that Aloe vera whole-leaf extract is an intestinal irritant in F344/N rats and B6C3F1 mice and a carcinogen of the large intestine in F344/N rats.

  8. Effect of dietary aloe vera on growth and lipid peroxidation indices in rainbow trout (Oncorhynchus mykiss).

    Science.gov (United States)

    Golestan, Ghazale; Salati, Amir Parviz; Keyvanshokooh, Saeed; Zakeri, Mohammad; Moradian, Hossein

    2015-01-01

    Aloe vera has been used worldwide in pharmaceutical, food and cosmetic industries due to the plethora of biological activities of its constituents. This study was done to evaluate the effects of dietary aloe vera on growth and lipid peroxidation in rainbow trout (Oncorhynchus mykiss). A total number of 480 O. mykiss (mean weight 9.50 ± 0.85 g) were randomized into four experimental groups including one control and three experimental groups that aloe vera was incorporated in their diet at 0.5, 1.0 and 2.0 g kg(-1). Trial was done for eight weeks. Then biometry and blood sampling were done. Plasma malondialdehyde, ferric reducing ability of plasma and growth index were estimated at the end of study. The results showed that aloe vera extract did not affect growth indices. Malondialdehyde was increased in the experimental group compared to the control group but ferric reducing ability of plasma showed a decrease in experimental groups (p aloe vera have adverse effects on antioxidant defense system in O. mykiss.

  9. Effect of pomegranate and aloe vera extract on streptococcus mutans: An in vitro study

    Directory of Open Access Journals (Sweden)

    Priya Subramaniam

    2012-01-01

    Full Text Available Introduction: The aim of the present in vitro study was to evaluate and compare the antibacterial effect of pomegranate and aloe vera extracts on Streptococcus mutans. Materials and Methods: Hydroalcoholic extracts of pulp from both Punica granatum (pomegranate and Aloe barbadensis miller (aloe vera were prepared to concentrations of 5, 25, 50 and 100%. Pure sorbitol powder dissolved in distilled water was taken as the negative control. Streptococcus mutans (S mutans was isolated from saliva by inoculation on to Mitus Salivarius Bacitracin (MSB agar, which was then streaked onto agar plates containing Brain Heart Infusion. In each petridish, wells were prepared and using a sterile micropipette, 125μl of the specific concentration of the extract (pomegranate/ aloe vera/ sorbitol was deposited in each well. This was done in triplicate for each concentration of the extracts. The effect of different concentrations of the extracts on S mutans was observed and the data was subjected to statistical analysis. Results: Pomegranate extract showed significantly higher inhibitory effect on S mutans at all concentrations (P≤0.05. On comparison of all three extracts at different concentrations, a significant difference (P≤0.05 was observed only at 50 and 100% concentrations. The inhibitory effect of pomegranate extract was significantly different when compared to aloe vera and sorbitol extracts. (P≤0.01. Discussion: Pomegranate extract has a significant antibacterial effect on S mutans at all concentrations.

  10. The Use of Aloe Vera Extract as a Novel Storage Media for the Avulsed Tooth

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    Samaneh Badakhsh

    2014-07-01

    Full Text Available Background: Tooth avulsion is one of the most severe dental traumas which most often occur in children. When immediate replantation is not possible, storage in a proper media may lead to a prolonged survival rate. Aloe Vera is a cactus like plant with green, tapered leaves that are filled with a transparent viscous gel. This medicinal plant has significant anti-inflammatory, antioxidant, antibacterial and antifungal effects. The purpose of this study was to assess the effectiveness of different concentrations of Aloe Vera extract compared to DMEM (cell culture medium and egg white. Methods: The periodontal ligament (PDL cells were cultured and certain number of cells were treated with Aloe Vera extract (in four different concentrations, egg white and culture media for 1, 3, 6, and 9 hours. Cell viability was determined by using the (3-[4, 5-dimethylthiazolyl-2]-2, 5-diphenyltetrazolium bromide assay. Moreover, One-way ANOVA and post hoc (LSD test were used for analyzing the study groups. Results: The results indicate that culture media and Aloe Vera extract (10, 30, and 50% concentration were statistically similar and significantly preserved more PDL cells compared to other experimental storage media. Conclusion: Aloe Vera 10, 30, and 50% may be recommended as a suitable storage media for avulsed teeth.

  11. Mechanism of Aloe Vera extract protection against UVA: shelter of lysosomal membrane avoids photodamage.

    Science.gov (United States)

    Rodrigues, Daniela; Viotto, Ana Cláudia; Checchia, Robert; Gomide, Andreza; Severino, Divinomar; Itri, Rosangela; Baptista, Maurício S; Martins, Waleska Kerllen

    2016-03-01

    The premature aging (photoaging) of skin characterized by wrinkles, a leathery texture and mottled pigmentation is a well-documented consequence of exposure to sunlight. UVA is an important risk factor for human cancer also associated with induction of inflammation, immunosuppression, photoaging and melanogenesis. Although herbal compounds are commonly used as photoprotectants against the harmful effects of UVA, the mechanisms involved in the photodamage are not precisely known. In this study, we investigated the effects of Aloe Vera (Aloe barbadensis mil) on the protection against UVA-modulated cell killing of HaCaT keratinocytes. Aloe Vera exhibited the remarkable ability of reducing both in vitro and in vivo photodamage, even though it does not have anti-radical properties. Interestingly, the protection conferred by Aloe Vera was associated with the maintenance of membrane integrity in both mimetic membranes and intracellular organelles. The increased lysosomal stability led to a decrease in lipofuscinogenesis and cell death. This study explains why Aloe Vera extracts offer protection against photodamage at a cellular level in both the UV and visible spectra, leading to its beneficial use as a supplement in protective dermatological formulations.

  12. Review of Clinical Pharmacology of Aloe vera L. in the Treatment of Psoriasis.

    Science.gov (United States)

    Miroddi, Marco; Navarra, Michele; Calapai, Fabrizio; Mancari, Ferdinando; Giofrè, Salvatore Vincenzo; Gangemi, Sebastiano; Calapai, Gioacchino

    2015-05-01

    Aloe vera L., is a plant used worldwide as folk remedy for the treatment of various ailments, including skin disorders. Its gel is present in cosmetics, medicinal products and food supplements. Psoriasis, an immune-mediated chronic inflammatory disease, involving mainly the skin, affects about the 2-3% of general population. Conventional pharmacological treatments for psoriasis can have limited effectiveness and can cause adverse reactions. For this reason often psoriatic patients look for alternative treatments based on natural products containing Aloe vera. We conducted a systematic review of clinical trials assessing effectiveness and safety of aloe for the treatment of psoriasis. Clinical studies published in English were considered; a total of four clinical trials met inclusion criteria. Studies were also evaluated by using the Jadad scale and Consort Statement in Reporting Clinical trials of Herbal Medicine Intervention. Quality and methodological accuracy of considered studies varied considerably, and some crucial information to reproduce clinical results was missing. We conclude that administration of aloe as cutaneous treatment is generally well tolerated, as no serious side effects were reported. Results on the effectiveness of Aloe vera are contradictory; our analysis reveals the presence of methodological gaps preventing to reach final conclusions.

  13. 芦荟药理研究进展%Aloe Vera Pharmacological Research

    Institute of Scientific and Technical Information of China (English)

    刘学华; 赵红影; 严令耕; 李瑞; 潘苗苗

    2011-01-01

    分别从芦荟的生物活性与有效成分研究、外用功能的药理研究、作用机制研究、毒副作用和安全性研究5个方面进行了综述,并对芦荟外用功能研究进行了评述.芦荟具有很大的药用价值,尤其是外用治疗应用广泛,近年来芦荟产品的开发研究倍受青睐,应用范围日趋扩大,成为全世界关注的药食多用植物.%Aloe vera is a kind of Aloe ( L.) perennial evergreen. Aloe vera has big medicinal value.Especially topical therapy is widely used, more the favour of people. In recent years, our product of the pharmacological studies, made gratifying achievements. For more in-depth study of the use function,this paper aloe vera respectively from the biological activity of effective ingredients and pharmacological research, external function, mechanism, toxicity and safety of five aspects were summarized, and the function of aloe vera external research are reviewed.

  14. Substitutional and Interstitial Diffusion in alpha2-Ti3Al(O)

    Science.gov (United States)

    Copland, Evan; Young, David J.; Gleeson, Brian; Jacobson, Nathan

    2007-01-01

    The reaction between Al2O3 and alpha2-Ti3Al was studied with a series of Al2O3/alpha2-Ti3Al multiphase diffusion couples annealed at 900, 1000 and 1100 C. The diffusion-paths were found to strongly depend on alpha2- Ti3Al(O) composition. For alloys with low oxygen concentrations the reaction involved the reduction of Al2O3, the formation of a gamma-TiAl reaction-layer and diffusion of Al and O into the alpha2-Ti3Al substrate. Measured concentration profiles across the interaction-zone showed "up-hill" diffusion of O in alpha2-Ti3Al(O) indicating a significant thermodynamic interaction between O and Al, Ti or both. Diffusion coefficients for the interstitial O in alpha2-Ti3Al(O) were determined independently from the interdiffusion of Ti and Al on the substitutional lattice. Diffusion coefficients are reported for alpha2-Ti3Al(O) as well as gamma-TiAl. Interpretation of the results were aided with the subsequent measurement of the activities of Al, Ti and O in alpha 2-Ti3Al(O) by Knudsen effusion-cell mass spectrometry.

  15. Anti-inflammatory and wound healing activities of Aloe littoralis in rats.

    Science.gov (United States)

    Hajhashemi, V; Ghannadi, A; Heidari, A H

    2012-04-01

    Aloe littoralis Baker (Asphodelaceae family) is a well known plant in southern parts of Iran. Because of its use in Iranian folk medicine as a wound-healing agent, the present study was carried out to investigate anti-inflammatory and wound healing activities of this plant in Wistar rats. A. littoralis raw mucilaginous gel (ALRMG) and also two gel formulations prepared from the raw mucilaginous gel were used in this study. Gel formulations (12.5% and 100% v/w Aloe mucilage in a carbomer base) were applied topically (500 mg once daily) for 24 days in the thermal wound model. Also Aloe gel formulation (100%) and ALRMG (500 mg daily) were evaluated in incisional wound model. Carrageenan-induced paw edema was used to assess the anti-inflammatory effect of intraperitoneal injection of ALRMG. In burn wound, ALRMG and Aloe formulated gel (100%) showed significant (P<0.05) healing effect. Topical application of ALMRG and Aloe formulated gel (100%) promoted healing rate of incisional wound. In carrageenan test, ALRMG (2.5 and 5 ml/Kg) revealed significant (P<0.05) anti-inflammatory activity. Results showed that A. littoralis is a potential wound-healing and anti-inflammatory agent in rats. Further studies are needed to find out the mechanism of these biological effects and also the active constituents responsible for the effects.

  16. Phytochemical standardization of Aloe vera extract by HPTLC techniques

    Institute of Scientific and Technical Information of China (English)

    Dinesh K Patel; Kanika Patel; SP Dhanabal

    2012-01-01

    Objective: To examine the phytochemical parameters of Aloe vera (A. vera) L. which can be used as a tool for its standardization. Methods: The phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and quantitative analysis of gallic acid and berberine by HPTLC method were included in present study. Results: Phytochemical analysis revealed the presence of alkaloid, carbohydrate, tannin, steroid, triterpenoid and glycoside. Total flavonoid and phenol content was found to be 1.9% and 13.11%. Concentartion of lead, arsenic, mercury and cadmium was found to be under the limit. Total bacterial count, yeast and moulds contents were found to be under the limit whereas Escherichia coli (E. coli) and salmonella was found to be absent in the extract. Quantitative analysis through HPTLC revealed the presence of 2.74%and 0.543% w/w of berberine and gallic acid. Conclusions: The results indicate that the plant extract are rich in berberine and gallic acid implying their importance to human health. This investigation could be used as source of standard parameters which can play an important role in its standardization.

  17. Processed Aloe vera Gel Ameliorates Cyclophosphamide-Induced Immunotoxicity

    Directory of Open Access Journals (Sweden)

    Sun-A Im

    2014-10-01

    Full Text Available The effects of processed Aloe vera gel (PAG on cyclophosphamide (CP-induced immunotoxicity were examined in mice. Intraperitoneal injection of CP significantly reduced the total number of lymphocytes and erythrocytes in the blood. Oral administration of PAG quickly restored CP-induced lymphopenia and erythropenia in a dose-dependent manner. The reversal of CP-induced hematotoxicity by PAG was mediated by the functional preservation of Peyer’s patch cells. Peyer’s patch cells isolated from CP-treated mice, which were administered PAG, produced higher levels of T helper 1 cytokines and colony-stimulating factors (CSF in response to concanavalin A stimulation as compared with those isolated from CP-treated control mice. PAG-derived polysaccharides directly activated Peyer’s patch cells isolated from normal mice to produce cytokines including interleukin (IL-6, IL-12, interferon-γ, granulocyte-CSF, and granulocyte-macrophage-CSF. The cytokines produced by polysaccharide-stimulated Peyer’s patch cells had potent proliferation-inducing activity on mouse bone marrow cells. In addition, oral administration of PAG restored IgA secretion in the intestine after CP treatment. These results indicated that PAG could be an effective immunomodulator and that it could prevent CP-induced immunotoxic side effects.

  18. Aloe vera-based formula as emollient on horses' hooves

    Directory of Open Access Journals (Sweden)

    Samuel Pereira Simonato

    2013-06-01

    Full Text Available The present study aimed at developing an Aloe vera-based formula for topical use on horse hoof and evaluating whether the treatment affects hooves growth and balance. Six healthy male horses between the ages of 3 and 17 years (12±5.25 were used, all semi-confined animals for breeding purposes. Before beginning A. vera treatment, animals underwent two trimming procedures with a 45 days-interval. After the second trimming, one of the forelimbs and one of the hindlimbs of 4 horses was weekly treated by topical application of the glycolic extract of A. vera at 20%. The contralateral limb, randomly chosen, received the extract at 50%. The hooves of the other animals were treated with propylene glycol. Treatment was done for 225 days and, during this time, animals underwent periodic trimming. Variables related to growth and balance of the hooves were measured before and after trimming. Data were analyzed using chi-square test and regression analysis at 5% significance. Growth rate of the hooves was not related to treatment. On the other hand, the 50% extract was related to the majority of the hooves in balance (p<0.05. Results suggest that a weekly topical treatment with A. vera glycolic extract does not improve the growth rate of the hooves; however, when applied at a high concentration, it improves their balance.

  19. Determinación de la sensibilidad a amoxicilina y a clindamicina de staphylococcus spp aislado de cavidad oral de pacientes con alto riesgo de endocarditis infecciosa

    Directory of Open Access Journals (Sweden)

    Silvia Barrientos

    2011-01-01

    Full Text Available En la literatura existen pocos estudios acerca de la distribución de Staphylococcus spp en boca y los que hay sobre estos microorganismos como residentes de la cavidad oral son controversiales.El género Staphylococcus spp conforma un importante grupo de patógenos en el ser humano y origina distintas enfermedades, entre ellas, la Endocarditis Infecciosa; de igual forma, la literatura reporta tasas de resistencia a amoxicilina y clindamicina antibióticos usados comúnmente para la profilaxis en odontología. Objetivo: Conocer la sensibilidad de Staphylococcus spp a amoxicilina y la clindamicina aislados de cavidad oral de pacientes con alto riesgo de Endocarditis Infecciosa.

  20. Feeding mice with Aloe vera gel diminishes L-1 sarcoma-induced early neovascular response and tumor growth.

    Science.gov (United States)

    Kocik, Janusz; Bałan, Barbara Joanna; Zdanowski, Robert; Jung, Leszek; Skopińska-Różewska, Ewa; Skopiński, Piotr

    2014-01-01

    Aloe vera (Aloe arborescens, aloe barbadensis) is a medicinal plant belonging to the Liliaceae family. Aloe vera gel prepared from the inner part of Aloe leaves is increasingly consumed as a beverage dietary supplement. Some data suggest its tumor growth modulatory properties. The aim of the present study was to evaluate in Balb/c mice the in vivo influence of orally administered Aloe vera drinking gel on the syngeneic L-1 sarcoma tumor growth and its vascularization: early cutaneous neovascular response, tumor-induced angiogenesis (TIA test read after 3 days), and tumor hemoglobin content measured 14 days after L-1 sarcoma cell grafting. Feeding mice for 3 days after tumor cell grafting with 150 μl daily dose of Aloe vera gel significantly diminished the number of newly-formed blood vessels in comparison to the controls. The difference between the groups of control and Aloe-fed mice (150 μl daily dose for 14 days) with respect to the 14 days' tumor volume was on the border of statistical significance. No difference was observed in tumor hemoglobin content.

  1. Four new species of Aloe (Aloaceae) from Ethiopia, with notes on the ethics of describing new taxa from foreign countries

    DEFF Research Database (Denmark)

    Demissw, Sebsebe; Friis, Ib; Awas, Tesfaye;

    2011-01-01

    Subsequent to the treatment of the Aloaceae, with 38 species of Aloe, in the Flora of Ethiopia (Sebsebe Demissew & Gilbert 1997), four more species, Aloe bertemariae Sebsebe & Dioli (2000), A. friisii Sebsebe & M. G. Gilbert (2000), A. clarkei L. E. Newton (2002) and A. elkerriana Dioli & T. A. M...

  2. Administration of dried Aloe vera gel powder reduced body fat mass in diet-induced obesity (DIO) rats.

    Science.gov (United States)

    Misawa, Eriko; Tanaka, Miyuki; Nabeshima, Kazumi; Nomaguchi, Kouji; Yamada, Muneo; Toida, Tomohiro; Iwatsuki, Keiji

    2012-01-01

    The aim of the present study was to investigate the anti-obesity effects of Aloe vera gel administration in male Sprague-Dawley (SD) rats with diet-induced obesity (DIO). SD rats at 7 wk of age were fed either a standard diet (10 kcal% fat) (StdD) or high-fat (60 kcal% fat) diet (HFD) during the experimental period. Four weeks after of HFD-feeding, DIO rats (11 wk of age) were orally administered with two doses of Aloe vera gel powder (20 and 200 mg/kg/d) for 90 d. Body weights (g) and body fat (%) of HFD fed rats were significantly higher than those of StdD-fed rats. Although a modest decrease of body weight (g) was observed with the administration of dried Aloe vera gel powder, both subcutaneous and visceral fat weight (g) and body fat (%) were reduced significantly in Aloe vera gel-treated rats. Serum lipid parameters elevated by HFD were also improved by the Aloe vera gel treatment. The oxygen consumption (VO(2)), an index of energy expenditure, was decreased in HFD-fed rats compared with that in StdD-fed rats. Administration of Aloe vera gel reversed the change in VO(2) in the HFD-fed rats. These results suggest that intake of Aloe vera gel reduced body fat accumulation, in part, by stimulation of energy expenditure. Aloe vera gel might be beneficial for the prevention and improvement of diet-induced obesity.

  3. Uso de cobertura com colágeno e aloe vera no tratamento de ferida isquêmica: estudo de caso El uso de colágeno y aloe vera en el tratamiento de la herida isquémica: estudio de caso Use of collagen and aloe vera in ischemic wound treatment: study case

    Directory of Open Access Journals (Sweden)

    Simone Helena dos Santos Oliveira

    2010-06-01

    Full Text Available O presente estudo relata o caso clínico de um paciente diabético e hipertenso, portador de ferida isquêmica, tratado com cobertura não convencional, à base de Aloe vera e colágeno. Faz parte de um projeto de pesquisa experimental, coordenado por professores e enfermeiros que atuam em projetos para o desenvolvimento de novas tecnologias para o tratamento de feridas. O caso em tela foi selecionado dentre os dos demais pacientes acompanhados. A coleta de dados foi efetuada através da anamnese e exame físico do paciente, utilizando-se um instrumento com dados relativos às condições do paciente e da lesão, bem como através do registro fotográfico da lesão. Os curativos foram realizados diariamente e, ao final de aproximadamente dez semanas, observou-se a total cicatrização da lesão. Não foram observados desconfortos ou complicações decorrentes do uso do produto, concluindo-se que o mesmo apresentou boa tolerabilidade e eficácia terapêutica para este caso em particular.Este estudio reporta el caso clínico de un paciente hipertenso, diabético, con una herida isquémica tratada con apósitos no convencionales de Aloe vera y colágeno, y forma parte de un proyecto de investigación experimental coordinado por profesores y enfermeros que trabajan en proyectos de desarrollo de nuevas tecnologías para el tratamiento de heridas. El caso de referencia fue elegido entre muchos pacientes en seguimiento. Los datos fueron obtenidos por la anamnesis y el examen físico del paciente, utilizándose un instrumento con datos relativos a las condiciones del paciente y de la lesión, así como un registro fotográfico de la lesión. Las curaciones se realizaron diariamente, al cabo de aproximadamente diez semanas se obtuvo la curación total. No se observó incomodidad o cualquier otra complicación derivada de la utilización del producto, por lo que se concluye en que el tratamiento demostró una buena tolerabilidad y eficacia terap

  4. Resistencia a antimicrobianos de uropatógenos aislados de pacientes ambulatorios atendidos en un laboratorio clínico de tercer nivel de complejidad de Bucaramanga, Santander

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    Karina Orduz-Pérez

    2016-08-01

    Full Text Available Objetivo: Determinar la resistencia a antimicrobianos de uropatógenos aislados de pacientes ambulatorios atendidos en el Laboratorio Clínico de Especialidades Bolívar S.A. de Bucaramanga, Santander. Metodología: Se realizó un estudio descriptivo retrospectivo (de Enero a Julio de 2009 a 1394 pacientes ambulatorios atendidos en el Laboratorio Clínico de Especialidades Bolívar S.A. de Bucaramanga Santander, con evidencia clínica o no de infecciones de tracto urinario (ITU y orden médica de Gram, urocultivo y antibiograma. Resultados: De los 1394 urocultivos realizados, se obtuvieron 589 pacientes con urocultivos positivos (42,3%; la mayoría correspondieron a uropatógenos Gram negativos; donde Eschericha coli fue el microorganismo más frecuente. Con respecto a los agentes utilizados en el tratamiento, el perfil de susceptibilidad encontrado para Eschericha coli como para otros microorganismos, mostró elevadas tasas de resistencia para Amoxacilina (59,1%, Ácido Nalidixico (52,1%, Cotrimoxazol (42,2% y Ciprofloxacina (40,7%. Las tasas de resistencia más bajas se observaron para antibióticos como Imipinem (0,8% y Fosfomicina (3,1%. Conclusiones: Eschericha coli sigue siendo el microorganismo más frecuentemente aislado a nivel mundial y el que ha presentado en los últimos años mayor resistencia a antibióticos. Con respecto al género más afectado el femenino sigue siendo el más vulnerable para estas infecciones por presentar mayores factores de riesgo.

  5. Producción y valoración de alimentos para animales monogástricos, con ensilado biológico de restos del procesamiento de langostino (Litopenaeus vannamei) fermentados con lactobacilos

    OpenAIRE

    Sánchez, Héctor; Universidad Nacional de Trujillo; Ochoa, Gloria

    2016-01-01

    El presente estudio permitió la valoración de dietas utilizando ensilado biológico de residuos del procesamiento de (Litopenaeus vannamei) EB, fermentados con inóculo de microorganismos comerciales (Lactobacillos acidofilos) o aislados del tracto digestivo del cerdo (Enterococcus hirae, Lactobacillus brevis, Lactobacillus johnsonii, Pediococcus pentosaceus) uso solo en lechones; preparación del EB, cocción de los residuos, molienda, mezcla (melaza e inóculo) e incubación; formulación de la di...

  6. The effects of crude aqueous and alcohol extracts of Aloe vera on growth and abdominal viscera of suckling rats.

    Science.gov (United States)

    Beya, Wabeya; Davidson, Bruce; Erlwanger, Kennedy H

    2012-01-01

    The gastrointestinal tract of neonates is sensitive to dietary manipulations. When nursing mothers use Aloe vera, their babies are at risk of indirect exposure to Aloe vera via breast feeding or directly as health supplements. The effects of orally administered extracts of Aloe vera in unweaned rats were investigated. Six day old Sprague-Dawley rats were gavaged with aqueous or alcohol extracts of Aloe vera (low dose 50mg. kg⁻¹ or high dose 500mg. kg⁻¹) daily for eight days. All data were expressed as mean ± SD and analyzed by one way ANOVA. Pups receiving high doses of either extract had a significantly higher body mass gain than the group receiving lower dose (p Aloe vera extracts resulted in growth promotion, enhanced hepatic storage of metabolic substrates, increased ALP possibly in relation to bone growth and caused hypertrophy of the caecum of neonatal rats. These effects need to be explored further to enhance animal production and health.

  7. Comparative evaluation of the effect of chlorhexidine and Aloe barbadensis Miller (Aloe vera) on dentin stabilization using shear bond testing

    Science.gov (United States)

    Sinha, Dakshita Joy; Jaiswal, Natasha; Vasudeva, Agrima; Garg, Paridhi; Tyagi, Shashi Prabha; Chandra, Priyanka

    2016-01-01

    Introduction: The main objective of adhesive dentistry is to create an effective, durable union between the tooth structure and restorative material. However, degradation of adhesive dentine interface remains largely responsible for the relatively short lifetime of tooth colored resin restoration. Aim: The aim of the study is to compare the dentin collagen stabilization property of Chlorhexidine (CHX) and Aloe barbadensis Miller using shear bond strength testing. Materials and Methods: Occlusal reduction was done in sixty extracted human mandibular molars to expose the mid coronal dentin and divided into three groups n = 20. Following the surface pretreatment (Group 1 = control, Group 2 = CHX, Group 3 = Aloevera), dentine bonding agent and composite resin were applied and cured. The specimens were then subjected to shear bond strength testing. Results: From the results analyzed, it was noted that there was statistically significant difference between the groups Control and CHX and Control and A. barbadensis Miller (P 0.05). Hence, the following result for the shear bond strengths to dentin was obtained: Control < CHX ≈ A. barbadensis Miller. Conclusion: CHX and A. barbadensis Miller, as pretreatment agents of acid demineralized dentin collagen, has no adverse effect on the immediate shear bond strength of a two-step etch and rinse adhesive to dentin. PMID:27656056

  8. Chemical and physical properties of aloe vera (Aloe barbadensis Miller gel stored after high hydrostatic pressure processing

    Directory of Open Access Journals (Sweden)

    Karina Di Scala

    2013-03-01

    Full Text Available The aim of this study was to evaluate the influence of high hydrostatic pressure (150, 250, 350, 450, and 550 MPa, applied for 5 minutes, on antioxidant capacity, total phenolic content, color, firmness, rehydration ratio, and water holding capacity of aloe vera gel stored for 60 days at 4 °C. The analyzed properties of the pressurized gel showed significant changes after the storage period. The highest value of total phenolic content was found at 550 MPa. However, a decrease in the antioxidant capacity was observed for all pressurized gel samples when compared to the control sample (p < 0.05. The smallest changes in product color were observed at pressure levels between 150 and 250 MP. The application of high hydrostatic pressure resulted in lower gel firmness, and the lowest value was found at 150 MPa (p < 0.05. On the other hand, the untreated sample showed a greater decrease in firmness, indicating that high pressure processing preserves this property. The application of high hydrostatic pressure exhibited modifications in the food matrix, which were evaluated in terms of rehydration ratio and water holding capacity.

  9. Aloe vera is non-toxic to cells: A microculture tetrazolium technique colorimetric assay study

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    Devi Gopakumar

    2014-01-01

    Full Text Available Introduction: Aloe vera (Av, a succulent of Liliaceae family is now a widely used medicinal plant. Its′ application covers a wide spectrum of human diseases, including oral mucosa, gastric mucosa and skin. Aloe vera preparations in the form of gel, mouth washes and cream are applied topically for many oral diseases. The applications include oral lichen planus, candidiasis, oral submucous fibrosis, geographic tongue, etc. Aims and Objectives: To evaluate the cytotoxicity of Av on human fibroblasts. Materials and Methods: Aloe vera preparation (70% was applied on the fibroblast cell lineage and the cell viability was evaluated by microculture tetrazolium technique (MTT colorimetric assay. Results: The cell viability at different concentrations was measured. The cells have maintained their viability at different concentrations used in the study. Conclusion: Our study shows the cell viability at different sample concentrations of Av. This could open up wide clinical applications of Av for reactive, inflammatory and potentially malignant oral and other mucocutaneous diseases.

  10. Effect of crosslinking in chitosan/aloe vera-based membranes for biomedical applications.

    Science.gov (United States)

    Silva, S S; Caridade, S G; Mano, J F; Reis, R L

    2013-10-15

    The positive interaction between polysaccharides with active phytochemicals found in medicinal plants may represent a strategy to create active wound dressing materials useful for skin repair. In the present work, blended membranes composed of chitosan (Cht) and aloe vera gel were prepared through the solvent casting, and were crosslinked with genipin to improve their properties. Topography, swelling, wettability, mechanical properties and in vitro cellular response of the membranes were investigated. With the incorporation of aloe vera gel into chitosan solution, the developed chitosan/aloe-based membranes displayed increased roughness and wettability; while the genipin crosslinking promoted the formation of stiffer membranes in comparison to those of the non-modified membranes. Moreover, in vitro cell culture studies evidenced that the L929 cells have high cell viability, confirmed by MTS test and calcein-AM staining. The findings suggested that both blend compositions and crosslinking affected the physico-chemical properties and cellular behavior of the developed membranes.

  11. RBF neural network prediction on weak electrical signals in Aloe vera var. chinensis

    Science.gov (United States)

    Wang, Lanzhou; Zhao, Jiayin; Wang, Miao

    2008-10-01

    A Gaussian radial base function (RBF) neural network forecast on signals in the Aloe vera var. chinensis by the wavelet soft-threshold denoised as the time series and using the delayed input window chosen at 50, is set up to forecast backward. There was the maximum amplitude at 310.45μV, minimum -75.15μV, average value -2.69μV and Aloe vera var. chinensis respectively. The electrical signal in Aloe vera var. chinensis is a sort of weak, unstable and low frequency signals. A result showed that it is feasible to forecast plant electrical signals for the timing by the RBF. The forecast data can be used as the preferences for the intelligent autocontrol system based on the adaptive characteristic of plants to achieve the energy saving on the agricultural production in the plastic lookum or greenhouse.

  12. Spectroscopic Constants of the X1Σ+ and 13Π states of AlO+

    Science.gov (United States)

    Sghaier, Onsi; Linguerri, Roberto; Mogren, Muneerah Mogren Al; Francisco, Joseph S.; Hochlaf, Majdi

    2016-08-01

    Using both standard and explicitly correlated ab initio methods in conjunction with several atomic basis sets, the ground state of AlO(X2Σ+) and the two lowest electronic states of AlO+ (1Σ+ and 3Π) are investigated. Potential energy curves for these species are mapped, which are incorporated later to solve the nuclear motion problem. Benchmark computations on AlO(X2Σ+) are used to determine the reliability of the theoretical methods and basis sets used for an accurate description of aluminum oxide compounds. The electronic ground state of AlO+ is X1Σ+, followed by the low-lying 13Π state. For both cationic electronic states, a set of spectroscopic parameters are recommended that may help in the identification of this ion in laboratory and astrophysical media. An accurate estimation of the adiabatic ionization energy of AlO, AIE = 9.70 eV, is also reported.

  13. Study on Effect of Aloe Glue on Cytogenetics, Cellular Immunity and Cell Proliferation of Human Cells

    Institute of Scientific and Technical Information of China (English)

    ZHANG Jiahua; WEN Shaluo; XIA Yun; ZHANG Lijun

    2002-01-01

    Objective To provide the scientific evidence for the exploiture of aloe resource. Methods Cytological combined determination was used to study the effect of aloe glue(0.01 ~ 0.3ml) on cytogenetics, cellular immunity and cell proliferation of human cells. Results SCE and MNR in varying dose groups had no significant differences as compared with control group( P > 0.05). LTR was significantly higher than that of control group(P < 0.005). MI was significantly higher than that of control group ( P < 0.05). M3 and PRI in highest dose group had significant differences as compared with control group (P < 0.05). Conclusion Aloe gel had no significant effect on cytogenetics. But it had activating effects on immunity and proliferation of cells.

  14. Una expresión para la fuerza hidrodinámica sobre un cuerpo interactuando con la estela del cuerpo de adelante

    OpenAIRE

    J. Ramírez-Muñoz; Soria, A. (Ainara)

    2007-01-01

    Presentamos un estudio hidrodinámico de la interacción de dos cuerpos esféricos del mismo diámetro alineados en la dirección de un flujo uniforme, newtoniano e incompresible. La solución asintótica para la estela de un cuerpo aislado se aplica a la interacción con un segundo cuerpo localizado en la estela laminar del cuerpo de adelante. Al igualar el defecto del flujo en la estela con el arrastre sobre el cuerpo y considerar el gradiente de presión, se obtuvo una expresión para la fuerza hidr...

  15. Estudio de la concentración microbiana del aire en depósitos del Archivo Nacional de Cuba

    Directory of Open Access Journals (Sweden)

    Sofía Flavia Borrego Alonso

    2010-09-01

    Full Text Available Actualmente el estudio de la microbiota en ambientes interiores es de interés para los especialistas, pues los microorganismos pueden provocar afectaciones a la salud de las personas y causar el biodeterioro de colecciones valiosas. Los objetivos del trabajo fueron: determinar la concentración microbiana del aire en depósitos del Archivo Nacional de Cuba, realizar la identificación taxonómica de los hongos aislados y definir el riesgo potencial que ellos representan para el biodeterioro del patrimonio documental y la salud del personal. El muestreo microbiológico se realizó en verano e invierno y en 6 depósitos, empleando un método de sedimentación. Para aislar hongos y bacterias se emplearon placas de Petri con medios de cultivo adecuados que se colocaron por triplicado a 3m de altura. Se determinó cualitativamente la actividad celulolítica, la producción de pigmentos y de ácidos a los hongos aislados. La microbiota viable total fue mayor en invierno que en verano, siendo la concentración bacteriana significativamente superior en esta estación (sequía en tanto que la fúngica es más elevada en verano. Los géneros fúngicos que predominaron en invierno fueron Aspergillus, Cladosporium, Penicillium, Curvularia y Alternaria, mientras que en verano prevaleció Cladosporium y se aislaron los géneros Fusarium, Mucor y Chrysonilia que llegaron a alcanzar niveles significativos en algunos depósitos. Los hongos aislados degradaron celulosa, produjeron pigmentos y ácidos. Asimismo, se evidenció que pueden provocar estados alérgicos y enfermedades respiratorias a las personas. Con relación a las bacterias del aire, predominaron las Gram positivas y se identificaron los géneros Enterobacter, Hafnia, Serratia, Streptomyces, Bacillus, Streptococcus y Staphylococcus. De igual manera, se detectó que las bacterias identificadas son capaces de producir afectaciones a la salud del personal.

  16. A Comparison between Antibacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an In Vitro Study).

    Science.gov (United States)

    Ehsani, Maryam; Amin Marashi, Mahmood; Zabihi, Ebrahim; Issazadeh, Maryam; Khafri, Soraya

    2013-01-01

    Removing the bacteria, including Enterococcus faecalis, from the root canal is one of the important aims in endodontic treatment.We aimed to compare the antibacterial activity of Chlorhexidine with two natural drugs. The antibacterial activities of three different propolis extracts (alcohol concentrations: 0, 15, 40%) and Aloe vera gel on E. faecalis were compared using three methods: disk diffusion, microdilution and direct contact test. In addition to the above bacterium, the Aloe vera gel effect on Staphylococcus aureus and Streptococcus mutans was evaluated. Disk diffusion test revealed that propolis ethanolic extracts (the alcohol concentration of 15 and 40%) and Aloe vera gel have antibacterial activities but aqueous extract of propolis did not show any effect in this test. The MICs for propolis ethanolic extracts, Aloe vera gel and aqueous extract of propolis (0% alcohol) were 313 µg/ml, 750 µg/ml, 2250 µg/ml, and ≥ 500 µg/ml respectively, much higher than the Chlorhexidine one. In direct contact test, contrary to Aloe vera, all three propolis extracts showed antibacterial effects on E. faecalis. The Aloe vera gel also showed significant antibacterial effect on S.aureus and S.mutans. The hydroalcoholic extracts of propolis and Aloe vera gel had antibacterial effects on E. faecalis, however, propolis is more potent than Aloe vera. The antibacterial effect of Aloe vera on S. aureus and S. mutans is low (MIC ≥ 2250 µg/ml). Appropriate concentrations of alcoholic extracts of propolis and some fractions of Aloe vera gel might be good choices for disinfecting the root canal in endodontic treatments.

  17. Phytomodulatory potentials of Aloe vera against Salmonella OmpR-mediated inflammation.

    Science.gov (United States)

    Rishi, Praveen; Rampuria, Anjali; Tewari, Rupinder; Koul, Ashwani

    2008-08-01

    Mediators released during inflammatory response play an essential role in eliminating microbes or microbial products. However, the uncontrolled release of cytotoxic substances characterized by extensive inflammation may adversely affect normal tissues. Under such conditions it is important to manage the hyperinflammation in order to change the clinical manifestations of the disease. Accordingly, the present study was designed to evaluate the modulation of Salmonella OmpR mediated inflammation by Aloe vera, a plant known to contain antiinflammatory ingredients. It was observed that outer-membrane proteins (OMPs) extracted from the wild type strain of S. typhimurium caused inflammation of greater magnitude compared with the OMPs extracted from its mutant construct as evident from the oedema test as well as the hyperalgesic (flicking) response of the animals under experimental conditions. However, Aloe vera applied topically, administered intraperitoneally or in combination modulated the inflammatory response. The maximum effect was observed with the combined formulation indicating modulation at local as well as systemic levels. The results reveal that this modulation could be due to the potential of Aloe vera to decrease peroxidative damage via a decrease in the levels of monokines (TNF-alpha, IL-1 and IL-6) and an increase in the level of superoxide dismutase (SOD). Moreover, the presence of SOD in Aloe vera itself might be responsible for enhancing its levels in the macrophages. On the other hand, no significant change in the catalase activity was observed by Aloe vera treatment. The use of Aloe vera, therefore, seems to have a promising role in the modulation of Salmonella OmpR mediated inflammation.

  18. Therapeutic approach by Aloe vera in experimental model of multiple sclerosis.

    Science.gov (United States)

    Mirshafiey, A; Aghily, B; Namaki, S; Razavi, A; Ghazavi, A; Ekhtiari, P; Mosayebi, G

    2010-09-01

    Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS) that leads to an inflammatory demyelination, axonal damage, and progressive neurologic disability that affects approximately 2.5 million people worldwide. The aim of the present research was to test the therapeutic effect of Aloe vera in experimental model of MS. All experiments were conducted on C57BL/6 male mice aged 6-8 weeks. To induce the experimental autoimmune encephalomyelitis (EAE), 250 microg of the myelin oligodendrocyte glycoprotein 35-55 peptide emulsified in complete freund's adjuvant was injected subcutaneously on day 0 over two flank areas. In addition, 200 ng of pertussis toxin in 100 microL phosphate buffered saline was injected intraperitoneally on days 0 and 2. The therapeutic protocol was carried out intragastrically using 120 mg/kg/day Aloe vera from 7 days before to 21 days after EAE induction. The mice were killed 21 days after EAE induction. The brains of mice were removed for histological analysis and their isolated splenocytes were cultured. The results indicated that treatment with Aloe vera caused a significant reduction in severity of the disease in experimental model of MS. Histological analysis showed 3 +/- 2 plaques in Aloe vera-treated mice compared with 5 +/- 1 plaques in control group. The density of mononuclear infiltration in the CNS of Aloe vera-treated mice (500 +/- 200) was significantly less in comparison to 700 +/- 185 cells in control group. Moreover, the serum level of nitric oxide in treatment group was significantly less than control animals. The level of interferon-gamma in cell culture supernatant of treated mice splenocytes was lower than control group, whereas decrease in serum level of interleukin-10 in treatment group was not significant in comparison with control mice. These data indicate that Aloe vera therapy can attenuate the disease progression in experimental model of MS.

  19. Evaluation of anti-inflammatory effect of aqueous extract of Aloe vera in Albino rats

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    Devarsi Choudhury

    2016-12-01

    Conclusions: In Adjuvant arthritis method for chronic inflammation, paw edema of the injected and the non-injected were measured on the 5th day and the 21st day respectively. Arthritic index was measured on the 21st day. These results were highly significant for AEAV and Indomethacin in comparison with the control. The results for the arthritic index were highly significant for both Aloe vera and Indomethacin. It is easily illustrated that the herbal plant Aloe vera at the dose of 500mg/kg demonstrated significant anti-inflammatory effect against all the experimental methods on inflammation. [Int J Basic Clin Pharmacol 2016; 5(6.000: 2488-2495

  20. Antifungal effects of different plant extracts and their major components of selected aloe species.

    Science.gov (United States)

    Ali, M I; Shalaby, N M; Elgamal, M H; Mousa, A S

    1999-08-01

    Different extracts of both fresh and dry leaves of Aloe eru A. Berger, A. vera L. Webb & Berth and A. arborescens Mill. were screened for their antifungal activity against Aspergillus niger, Cladosporium herbarum and Fusarium moniliforme. The toxicity of the isolated pure components were evaluated on the tested fungi. A comparative chromatographic study was performed to differentiate between natural components existing in various fractions and extracts of Aloe species and specific spray reagents were used for the detection of anthraquinones in the isolated components.

  1. Pharmacological attribute of Aloe vera: Revalidation through experimental and clinical studies.

    Science.gov (United States)

    Gupta, Vinay K; Malhotra, Seema

    2012-04-01

    Aloe vera has long been used as a traditional medicine for inducing wound healing. It is a natural product that now a days is used in cosmetic industry. Benefits associated with Aloe vera have been attributed to the polysaccharides contained in the gel of the leaves though there are various indications for its use. Biological activities include promotion of wound healing, antifungal activity, anti-inflammatory, anticancer and immunomodulatory. Gingival fibroblasts play an important role in oral wound healing. Double blind-controlled trials are needed to determine its real efficacy in oral health.

  2. Effects of Aloe vera on dressing percentage and haemato-biochemidal parameters of broiler chickens

    OpenAIRE

    Jagmohan Singh; K.M. Koley; Khushboo Chandrakar; Nileshkumar S. Pagrut

    2013-01-01

    Aim: To evaluate the effects of Aloe vera on dressing percentage and hemato-biochemical parameters of broiler chickens.Materials and Methods: A total of 90 chicks were used in this study. They were randomly allocated into 3 treatment groups.Fresh Aloe vera leaf juice (ALJ) was prepared and administered to the test group T3 at the rate of 20 g/Lin drinking water daily.This study was carried out for 42 days. Dressing percentage and hemato-biochemical parameters were recorded at the end ofexperi...

  3. Comportamiento de la concentración microbiana aérea en la Fototeca del Archivo Nacional de Cuba

    OpenAIRE

    Sofía Borrego-Alonso; Michel García-Miniet

    2011-01-01

    Los objetivos del estudio fueron evaluar el predominio microbiano en el aire interior de la Fototeca del Archivo Nacional de la República de Cuba, realizar la caracterización fisiológica de los hongos aislados para determinar su potencial biodeteriorante y analizar a partir de la información especializada, las características patogénicas de los microorganismos que se aislaron con mayor frecuencia. En el muestreo microbiológico se utilizó un método de sedimentación y para ello, se emplearon pl...

  4. Relación existente entre la infección por los diferentes genotipos del Virus del Papiloma Humano y la presencia de patología premaligna y maligna del cuello uterino

    OpenAIRE

    Mazarico Gallego, Edurne

    2012-01-01

    1) Introducción Hasta el momento se han secuenciado total o parcialmente más de 100 tipos y subtipos de VPH. De todos ellos, aproximadamente 40 tipos se han aislado en lesiones de tracto genital inferior y unos 35, según diferentes estudios, en carcinomas. Según su riesgo oncogénico, se clasifican en tipos de VPH de bajo riesgo (VPH-BR), VPH de probable alto riesgo y VPH de alto riesgo (VPH-AR). Uno de los descubrimientos más importantes en la investigación etiológica del cáncer en los...

  5. Cálculo de la resistencia a la penetración en hojas de aloe vera (Barbadenses Miller

    Directory of Open Access Journals (Sweden)

    Giovanni Torres Charry

    2013-12-01

    Full Text Available Para caracterizar la resistencia a la penetración de hojas de aloe vera, variedad Barbadenses Miller, se diseñó una máquina y se establecieron los protocolos de medición para desplazamiento y fuerza necesarios para medir la resistencia a la penetración en tres puntos diferentes de las hojas. Los resultados mostraron que no existe una relación lineal entre el desplazamiento del indentador en la hoja y la fuerza de penetración. Por otro lado, se encontró que no existen diferencias en los valores de la fuerza de penetración al evaluar ésta en distintos puntos para una misma hoja. Se hallaron diferencias en los valores obtenidos entre hojas diferentes, lo que permite suponer que existe influencia de las características dimensionales de las hojas en el valor de la fuerza de penetración. Los valores máximos obtenidos para la fuerza de penetración variaron entre 22 y 27 N, para una velocidad de avance de la herramienta de 2.6 mm/s.

  6. Optimality in Microwave-Assisted Drying of Aloe Vera ( Aloe barbadensis Miller) Gel using Response Surface Methodology and Artificial Neural Network Modeling

    Science.gov (United States)

    Das, Chandan; Das, Arijit; Kumar Golder, Animes

    2016-10-01

    The present work illustrates the Microwave-Assisted Drying (MWAD) characteristic of aloe vera gel combined with process optimization and artificial neural network modeling. The influence of microwave power (160-480 W), gel quantity (4-8 g) and drying time (1-9 min) on the moisture ratio was investigated. The drying of aloe gel exhibited typical diffusion-controlled characteristics with a predominant interaction between input power and drying time. Falling rate period was observed for the entire MWAD of aloe gel. Face-centered Central Composite Design (FCCD) developed a regression model to evaluate their effects on moisture ratio. The optimal MWAD conditions were established as microwave power of 227.9 W, sample amount of 4.47 g and 5.78 min drying time corresponding to the moisture ratio of 0.15. A computer-stimulated Artificial Neural Network (ANN) model was generated for mapping between process variables and the desired response. `Levenberg-Marquardt Back Propagation' algorithm with 3-5-1 architect gave the best prediction, and it showed a clear superiority over FCCD.

  7. Optimality in Microwave-Assisted Drying of Aloe Vera (Aloe barbadensis Miller) Gel using Response Surface Methodology and Artificial Neural Network Modeling

    Science.gov (United States)

    Das, Chandan; Das, Arijit; Kumar Golder, Animes

    2016-07-01

    The present work illustrates the Microwave-Assisted Drying (MWAD) characteristic of aloe vera gel combined with process optimization and artificial neural network modeling. The influence of microwave power (160-480 W), gel quantity (4-8 g) and drying time (1-9 min) on the moisture ratio was investigated. The drying of aloe gel exhibited typical diffusion-controlled characteristics with a predominant interaction between input power and drying time. Falling rate period was observed for the entire MWAD of aloe gel. Face-centered Central Composite Design (FCCD) developed a regression model to evaluate their effects on moisture ratio. The optimal MWAD conditions were established as microwave power of 227.9 W, sample amount of 4.47 g and 5.78 min drying time corresponding to the moisture ratio of 0.15. A computer-stimulated Artificial Neural Network (ANN) model was generated for mapping between process variables and the desired response. `Levenberg-Marquardt Back Propagation' algorithm with 3-5-1 architect gave the best prediction, and it showed a clear superiority over FCCD.

  8. Systematic Error Analysis of Direct Georeferencing for ALOS PRISM Imagery%ALOS PRISM影像直接定位的系统误差分析

    Institute of Scientific and Technical Information of China (English)

    雷蓉; 范大昭; 刘楚斌; 马秋禾

    2011-01-01

    利用ALOS PRISM影像进行直接对地定位时必须考虑系统误差的检校.从ALOS卫星PRISM传感器的成像原理出发,分析了利用严格几何模型进行定位时可能存在的系统误差;然后用3个不同的检校模型对系统误差进行校正.实验表明利用少量控制点剔除系统误差后,定位精度明显提高,在X方向达到3m左右,在Y方向和Z方向不超过2 m.%The calibration of constant error must be taken into account by using the ALOS PRISM Imagery for direct georeferencing. From the imaging theory of the ALOS PRISM sensor, the systematic errors that may be exist by using the rigorous geometric model for direct georeferencing were analyzed. Three different calibration models were used to analyze and correct the systematic errors. Experimental results showed that there were constant systematic errors existed in imagery ancillary data. Once the systematic errors are eliminated using some ground control points, the results are improved noticeably. The indices is about 3 m in X direction, better than 2 m in Y direction and Z direction.

  9. An in vitro and in vivo toxicologic evaluation of a stabilized aloe vera gel supplement drink in mice.

    Science.gov (United States)

    Sehgal, Inder; Winters, Wallace D; Scott, Michael; Kousoulas, Konstantine

    2013-05-01

    Aloe vera gel is increasingly consumed as a beverage dietary supplement. The purpose of this study was to determine potential toxicity of a stabilized aloe vera gel derived from the inner gel fillet and marketed as a drink. The gel juice was assessed through assays of genotoxicity in vivo and acute and subchronic toxicity in B6C3F1 mice. Aloe vera did not increase the SOS DNA repair response in Escherichia coli and at 1× and 0.25× it did not increase mutagenesis of Salmonella TA100 resulting in histidine biosynthesis. At 3 and 14days following acute exposure, male and female mice gavaged with the stabilized aloe gel had daily appearances, total body weight gain, selected organ weights, necropsy and hematology tests similar to control mice gavaged with water. After a 13-week aloe gel feed study, male and female mice evaluated by the same criteria as the acute study plus feed consumption and serum chemistry tests were found to be equivalent to control groups. These data indicate that a commercial stabilized aloe gel consumed as a beverage was not genotoxic or toxic in vivo. These results contrast with those obtained using preparations containing aloe latex phenolic compounds such as anthraquinones.

  10. A comparative study of baby immature and adult shoots of Aloe vera on UVB-induced skin photoaging in vitro.

    Science.gov (United States)

    Hwang, Eunson; Kim, Su Hyeon; Lee, Sarah; Lee, Choong Hwan; Do, Seon-Gil; Kim, Jinwan; Kim, Sun Yeou

    2013-12-01

    Ultraviolet (UV) irradiation induces photo-damage of the skin, which in turn causes depletion of the dermal extracellular matrix and chronic alterations in skin structure. Skin wrinkle formations are associated with collagen synthesis and matrix metalloproteinase (MMP) expression. The production of type I procollagen is regulated by transforming growth factor-β1 (TGF-β1) expression; the activation of MMP is also correlated with an increase of interleukin-6 (IL-6). Aloe barbadensis M. (Aloe vera) is widely used in cosmetic and pharmaceutical products. In this study, we examined whether baby aloe shoot extract (BAE, immature aloe extract), which is from the one-month-old shoots of Aloe vera, and adult aloe shoot extract (AE), which is from the four-month-old shoots of Aloe vera, have a protective effect on UVB-induced skin photoaging in normal human dermal fibroblasts (NHDFs). The effects of BAE and AE on UVB-induced photoaging were tested by measuring the levels of reactive oxygen species, MMP-1, MMP-3, IL-6, type I procollagen, and TGF-β1 after UVB irradiation. We found that NHDF cells treated with BAE after UVB-irradiation suppressed MMP-1, MMP-3, and IL-6 levels compared to the AE-treated cells. Furthermore, BAE treatment elevated type I procollagen and TGF-β1 levels. Our results suggest that BAE may potentially protect the skin from UVB-induced damage more than AE.

  11. Aloe chemical constituents and pharmacological research%芦荟的化学成分及药理作用

    Institute of Scientific and Technical Information of China (English)

    翟莲; 宋凯凯

    2014-01-01

    芦荟作为一种常见的药用植物,已越来越受到人们的关注。其为百合科植物库拉索芦荟Aloe barbadensisMil er、好望角芦荟Aloe feroxMil er或其他同属近缘植物叶的汁液浓缩干燥物,含有蒽醌类、糖类、维生素类、有机酸类等多种有效成分,具有通便泻下、抗肿瘤、抗糖尿病等多种的药理作用。%Aloe vera as a common medicinal plants, has been more and more attention. The lily family plant Aloe vera Aloe barbadensisMil er, Cape Aloe Aloe feroxMil er same relatives or other juice concentrate dried leaf material, containing anthraquinone, carbohydrate, vitamins, organic acids and other active ingredients with constipation purgative, anti-tumor, anti-diabetic and other pharmacological effects.

  12. Effect of Aloe vera on some indicators of cell damage after a period of aerobic exercise in male athletes

    Directory of Open Access Journals (Sweden)

    Barati Amir-Hosein

    2015-07-01

    Full Text Available Introduction: Aloe vera is a medicinal plant with antioxidant activity. The purpose of this study was to determine the effect of Aloe vera on some indicators of cell damage after a period of aerobic exercise in male athletes of 15-18 years old. Methods: In this clinical trial study, 20 male students with average weight of 64.85 ± 51 and height of 172.05 ± 6.4 were randomly assigned to 2 groups of Aloe vera supplement (n = 10 and placebo (n = 10. Then aerobic training was conducted for 4 weeks. The supplemented group took 3 capsules, each capsule contains 2 g of dried Aloe vera and placebo group took 3 capsules/day containing dextran after every meal. To determine the index of cell injury markers creatine kinase (CK, lactate dehydrogenase (LDH and reactive protein C (CRP were determined and blood samples were collected 24 hours before and after each test. The data were analyzed using ANOVA and independent t test. Significance level was considered as P ≤ .05. Results: The use of Aloe vera during aerobic exercise significantly reduced LDH (P = .006 in the supplement group (15% reduction compared to placebo group and CRP with 11% reduction in the supplement group compared to placebo (P = .008. There was no significant reduction in CK. Conclusion: The findings of this study showed that Aloe vera reduces cell damage and inflammation indicators. This result may reflect the role of Aloe vera as anti-inflammatory and antioxidant agent.

  13. Consorcios microbianos con actividad ácido-láctica promisoria aislados desde inoculantes bacterianos nativos para ensilajes

    Directory of Open Access Journals (Sweden)

    Byron Leoncio Díaz-Monroy

    2015-04-01

    Full Text Available El objetivo de este trabajo fue aislar y caracterizar cepas de bacterias ácido lácticas (BAL a partir de preparados microbianos (PM nativos elaborados a base de residuos agroindustriales, como suero de leche, estiércol bovino y contenido ruminal, y diseñados como inoculantes biológicos para procesos de ensilaje de residuales orgánicos poscosecha. Se evaluaron 5 tratamientos (preparados microbianos, PM + 33% de residuo inoculante, con 3 réplicas: 1 Tratamiento control sin inoculante (PMSI, 2 Suero de leche (PMSL, 3 Estiércol bovino (PMEB, 4 Combinación de suero+estiércol (PMM y 5 Contenido ruminal (PMCR; además, en todos se adicionó: melaza (20%, urea (1%, sal mineral (1% y agua (45%. En recipientes plásticos se fermentaron tapados durante 96 horas, a 14 °C; de allí se obtuvieron 3 muestras de 500 mL de cada PM, se sembraron en agar MRS (Man, Rogosa y Sharpe y se incubaron a 37 ºC durante 72 h bajo condiciones anaerobias (AnaeroGen, Oxoid. Las cepas de BAL son catalasa y oxidasa negativas, cocos, coco-bacilos y bacilos Gram positivos no esporulados. La identificación bioquímica se realizó con el sistema API-50CHL (BioMérieux, France y la base de datos APILAB Plus versión 3.3.3. Se aislaron 39 cepas de BAL, 4 del PMSI (2 Lactobacillus plantarum, 1 L. brevis y 1 Lactococcus lactis, 12 del PMSL (3 Lactobacillus rhamnosus, 3 L. casei, 2 L. acidophilus, 2 L. paracasei y 2 L. fermentum, 7 del PMEB (3 Streptococcus bovis, 2 Lactobacillus plantarum y 2 Leuconostoc mesenteroides, 10 del PMM (3 Lactobacillus casei, 2 L. plantarum, 2 L. acidophilus, 1 L. fermentum, 1 L. rhamnosus y 1 L. paracasei y 6 del PMCR (2 Lactobacillus vitulinus, 2 L. ruminus, 1 L. reuteri y 1 Pediococcus damnosus. En conclusión, la mejor fuente de cepas de BAL fue PMSL, que le otorga ventajas como posible inoculante bacteriano para ensilajes. En todos los PM se identificaron cepas homofermentativas y heterofermentativas, asociadas en consorcios

  14. Validation of burst overlapping for ALOS-2 PALSAR-2 ScanSAR-ScanSAR interferometry

    Science.gov (United States)

    Natsuaki, Ryo; Motohka, Takeshi; Ohki, Masato; Watanabe, Manabu; Suzuki, Shinichi

    2016-10-01

    The Phased Array type L-band Synthetic Aperture Radar-2 (PALSAR-2) aboard the Advanced Land Observing Satellite- 2 (ALOS-2, "DAICHI-2") is the latest L-band spaceborne synthetic aperture radar (SAR). PALSAR-2 observes the world mainly with 10 m resolution / 70 km swath Stripmap mode and 25 m resolution / 350 km swath ScanSAR mode. The 3-m resolution Stripmap mode is mainly used upon Japan. 350 km ScanSAR observation could detect large scale deformation e.g., the Mw 7.8 Gorkha, Nepal earthquake and its aftershocks in 2015. ALOS-2 ScanSAR is the first one that supports ScanSAR-ScanSAR interferometry in L-band spaceborne SAR. However, because of the parameter setting error for the orbit estimation, ALOS-2 PALSAR-2 ScanSAR could achieve little number of interferometric pair until the software modification on February 8, 2015. That is, the burst overlap timing required for the interferometric analysis was insufficient and it depends on the observation date. In this paper, we report the investigation results of this case and discuss the current status of the ALOS-2 ScanSAR InSAR. Some archives achieved before February 8, 2015 can be used for interferometric analysis with after Feb. 8. However, most of them have no interferometric pair. We also report that the archives acquired after February 8, have enough burst overlapping.

  15. Comparative evaluation of natural antioxidants spirulina and aloe vera for the treatment of oral submucous fibrosis

    Science.gov (United States)

    Patil, Santosh; Al-Zarea, Bader Kureyem; Maheshwari, Sneha; Sahu, Rohit

    2015-01-01

    Aim Oral submucous fibrosis (OSMF) is a high risk premalignant condition predominantly seen in the Indian subcontinent. The aim of the present study was to compare the efficacy of spirulina and aloe vera in the management of OSMF. Material and methods 42 subjects with clinico-pathologically diagnosed OSMF were included in the study and divided equally in 2 groups, Group A (spirulina group) and Group B (aloe vera group). Group A was administered 500 mg spirulina in 2 divided doses for 3 months and Group B was given 5 mg aloe vera gel to be applied topically thrice daily for 3 months. Evaluation for different clinical parameters was done at regular intervals and data was analyzed using the Chi-square test. P-value <0.05 was considered to be statistically significant. Results The patients in Group A showed significant clinical improvement in mouth opening and ulcers/erosions/vesicles (p < 0.05). However, there was no significant improvement in burning sensation (p = 0.06) and pain associated with the lesion (p = 0.04) among the 2 groups. Conclusion Both the drugs showed improvement in the condition; however spirulina can bring about significant clinical improvements in the symptoms like mouth opening and ulcers/erosion/vesicles. Thus, spirulina appears to be more promising when compared to aloe vera for the treatment of OSMF. PMID:25853042

  16. Disinfection of dental unit water line using aloe vera: in vitro study.

    Science.gov (United States)

    Pareek, Sonia; Nagaraj, Anup; Sharma, Prateek; Atri, Mansi; Walia, Satinder; Naidu, Shravani; Yousuf, Asif

    2013-01-01

    Context. Dental unit waterlines may be heavily contaminated with microorganisms and are a potential source of infection for both practicing staff and immunocompromised patients particularly. Contamination of dental unit water lines could be inhibited with the use of disinfectants. The present study investigates the effect of aloe-vera-based disinfectant in reducing the microbial growth in dental unit water lines (DUWLs). Aims. To compare the efficacy of aloe vera, hydrogen peroxide (H2O2), and 5% sodium hypochlorite (NaOCl) in controlling microbial contamination of DUWLs. Materials and Methods. After obtaining baseline water samples, the dental unit waterlines were treated with aloe vera, 10% hydrogen peroxide, and 5% sodium hypochlorite. Each of the three disinfectants was used in increasing concentrations and their inhibiting effect was compared. Water samples were analyzed for microbiological quality by the total viable count (TVC) method. Statistical Analysis Used. SPSS 16. Results. There was significant reduction in mean CFU/ml when treated with disinfectants each for a period of one week. Aloe-vera solution was found to be the most effective in reducing the microbial colonies. Conclusions. Improving the water quality from dental unit water lines is of considerable importance; chemical-based disinfectants can be replaced with herbal disinfectants for treating microbial contamination in dental unit waterlines.

  17. Disinfection of Dental Unit Water Line Using Aloe Vera: In Vitro Study

    Directory of Open Access Journals (Sweden)

    Sonia Pareek

    2013-01-01

    Full Text Available Context. Dental unit waterlines may be heavily contaminated with microorganisms and are a potential source of infection for both practicing staff and immunocompromised patients particularly. Contamination of dental unit water lines could be inhibited with the use of disinfectants. The present study investigates the effect of aloe-vera-based disinfectant in reducing the microbial growth in dental unit water lines (DUWLs. Aims. To compare the efficacy of aloe vera, hydrogen peroxide (H2O2, and 5% sodium hypochlorite (NaOCl in controlling microbial contamination of DUWLs. Materials and Methods. After obtaining baseline water samples, the dental unit waterlines were treated with aloe vera, 10% hydrogen peroxide, and 5% sodium hypochlorite. Each of the three disinfectants was used in increasing concentrations and their inhibiting effect was compared. Water samples were analyzed for microbiological quality by the total viable count (TVC method. Statistical Analysis Used. SPSS 16. Results. There was significant reduction in mean CFU/ml when treated with disinfectants each for a period of one week. Aloe-vera solution was found to be the most effective in reducing the microbial colonies. Conclusions. Improving the water quality from dental unit water lines is of considerable importance; chemical-based disinfectants can be replaced with herbal disinfectants for treating microbial contamination in dental unit waterlines.

  18. Aloe vera for prevention of radiation-induced dermatitis: a self-controlled clinical trial.

    Science.gov (United States)

    Haddad, P; Amouzgar-Hashemi, F; Samsami, S; Chinichian, S; Oghabian, M A

    2013-08-01

    To evaluate an Aloe vera lotion for prevention of radiation-induced dermatitis, all patients with a prescription of radiotherapy to a minimum dose of 40 Gy were eligible provided that their treatment area could be divided into two symmetrical halves. Patients were given a lotion of Aloe vera to use on one half of the irradiated area, with no medication to be used on the other half. The grade of dermatitis in each half was recorded weekly until 4 weeks after the end of radiotherapy. The trial enrolled 60 patients (mean age: 52 years; 67% women). Most patients had breast cancer (38%), followed by pelvic (32%), head-and-neck (22%), and other cancers (8%). Field size was 80-320 cm(2) (mean: 177 cm(2)), and the dose of radiotherapy was 40-70 Gy (mean: 54 Gy). Concurrent chemotherapy was administered in 20 patients. From week 4 to week 6 of radiotherapy and then at weeks 2 and 4 after radiotherapy, the mean grade of dermatitis with and without Aloe vera was 0.81 and 1.10 (p Aloe vera reduces the intensity of radiationinduced dermatitis.

  19. In vitro phytochemical characteristics and antioxidant capacity of Aloe vera, Plukenetia volubilis, Caiophora carduifolia, Cecropia membranacea

    OpenAIRE

    Franco Quino, César; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Laboratorio de Fisiología y Farmacología, Facultad de Odontología, UNMSM, Lima, Perú; Muñoz Espinoza, Danny; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Facultad de Ciencias Químicas, Universidad de Guayaquil, Ecuador; Gómez Herreros, César; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Chau Miranda, Gina; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Cueva Piña, Liliana; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Guardia Ortiz, Eliza; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Saavedra Yucra, Santos; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Arroyo Acevedo, Jorge; Laboratorio de Farmacología Experimental, Facultad de Medicina Humana, UNMSM, Lima, Perú; Instituto de Investigaciones Clínicas, UNMSM, Lima, Perú; Herrera Calderón, Oscar; Facultad de Fisiología y Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad Nacional San Luis Gonzaga, Ica, Perú

    2016-01-01

    Introduction. Antioxidants have shown chemopreventive potential in degenerative, inflammatory, autoimmune, oncology and respiratory distress associated pathologies. Objective. To assess the phytochemical and antioxidant properties in vitro by DPPH and ABTS method. Design. Observational analytical. Location. Laboratory of Experimental Pharmacology, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological/Chemical Material. Aloe vera leaves, Plukenetia volubilis see...

  20. TECHNOLOGICAL ASPECTS CONCERNING PRODUCTION ON ASEXUAL TRACK OF ORNAMENTAL CULTURE OF ALOE ARBORESCENS

    Directory of Open Access Journals (Sweden)

    Mădălina Doltu

    2012-12-01

    Full Text Available Research conducted in culture of Aloe arborescens Mill., in conditions of protected area (greenhouse, had to watch some of technological aspects to obtain some in pot ornamentals plants, competitive plants for european market. Experience has included plants of Aloe arborescens Mill., aged 1-5 years, obtained by vegetative multiplying, asexual. Production technology was applied to aloe plants (multiplying, transplanting, maintenance, taking into account the ecological requirements of specie and possibilities of using automated artificial factors influencing microclimate (shading, ventilation, cooling of production space. Researches has established technological aspects concerning production of plants, to satisfy requirements of specie, for obtained some specimens by superior decorative quality. The results of experienced technology indicate quality of morphological characters, growth increases important of decorative elements (height, leaves, shoots, appearance of flowering at plants aged two years. Culture of Aloe arborescens Mill. in pot, can be practiced all year, just in conditions of protected areas. This culture, realized in the purpose for obtain decorative plants, is a valuable activity of horticulture.

  1. Economic Sustainability of Organic Aloe Vera Farming in Greece under Risk and Uncertainty

    Directory of Open Access Journals (Sweden)

    Angelos Liontakis

    2016-04-01

    Full Text Available During the last decade, an encouraging environment for the restructuring and modernization of the agricultural sector has formed in Greece. The diversification into higher-value crops can be a promising option for small and average-sized farms, particularly during the current economic crisis. One of the most promising alternative crops that have been recently established in Greece is the organic Aloe vera crop. The main advantage of this crop is that it can utilize poor farmlands and, therefore, can facilitate rural development in marginal areas. This study explores the economic sustainability of the Aloe vera crop, considering the embedded risk and uncertainty. The results indicate that organic aloe farming is a promising alternative to “traditional” crops in Greece, particularly for family farms in rural areas. In contrast, this activity is not advisable to the most entrepreneurial type of farmers, unless their crop size allows economies of scales. Finally, the Stochastic Efficiency with Respect to a Function (SERF analysis associates farmers’ risk attitude with their willingness to be involved in organic Aloe vera farming. SERF analysis highlights the crucial role of farmers’ risk aversion and concludes that, above a certain level of risk aversion, farmers have no incentive to adopt this economic activity.

  2. Protective effects of Aloe vera-based diets in Eimeria maxima-infected broiler chickens

    Science.gov (United States)

    Aloes have been widely used for a broad range of pharmacological activities, including parasitic problems. Avian coccidiosis is the most costly and wide-spread parasitic disease in the poultry industry, and has been mainly controlled by the use of chemotherapeutic agents. Due to the emergence of dru...

  3. Characterization of gelatin/chitosan scaffold blended with aloe vera and snail mucus for biomedical purpose.

    Science.gov (United States)

    López Angulo, Daniel Enrique; do Amaral Sobral, Paulo José

    2016-11-01

    Biologically active scaffolds used in tissue engineering and regenerative medicine have been generating promising results in skin replacement. The present study aims to test the hypothesis that the incorporation of Aloe vera and snail mucus into scaffolds based on gelatin and chitosan could improve their structure, composition and biodegradability, with a potential effect on bioactivity. Homogeneous pore diameter as well as pore walls in the composite scaffold could be seen in the SEM image. The pores in the scaffolds were interconnected and their sizes ranged from 93 to 296μm. The addition of Aloe vera and snail mucus enlarged the mean pore size with increased porosity and caused changes in the pore architecture. The FTIR analysis has shown good affinity and interaction between the matrix and the Aloe, which may decrease water-binding sites, so this fact hindered the water absorption capacity of the material. The mechanical properties could explain the highest swelling capacity of the snail scaffold, because the high percentage of elongation could facilitate the entry of liquid in it, generating a matrix with plenty of fluid retention. The real innovation in the present work could be the use of these substances (Aloe and snail mucus) for tissue engineering.

  4. Effects of Aloe vera on dressing percentage and haemato-biochemidal parameters of broiler chickens

    Directory of Open Access Journals (Sweden)

    Jagmohan Singh

    2013-09-01

    Full Text Available Aim: To evaluate the effects of Aloe vera on dressing percentage and hemato-biochemical parameters of broiler chickens.Materials and Methods: A total of 90 chicks were used in this study. They were randomly allocated into 3 treatment groups.Fresh Aloe vera leaf juice (ALJ was prepared and administered to the test group T3 at the rate of 20 g/Lin drinking water daily.This study was carried out for 42 days. Dressing percentage and hemato-biochemical parameters were recorded at the end ofexperiment.Results: Group that was given Aloe vera (T3 showed numerically higher dressing percentage as compared to control group(T1 and drug control group (T2. It also showed significantly (P0.05 differences were observed in other parameters among all the treatment groups.Conclusion: Aloe vera has potential to be a growth promoter in broiler chicks and its growth promoting effects are comparableto that of antibiotic growth promoter (AGP.

  5. Expression of biologically active human interferon alpha 2 in aloe vera

    Science.gov (United States)

    We have developed a system for transgenic expression of proteins in Aloe Vera. Using this approach we have generated plants expressing the human gene interferon alpha 2, IFNa2. IFNa2 is a small secreted cytokine that plays a vital role in regulating the body’s immune response to viral infections a...

  6. Facile fabrication of aloe vera containing PCL nanofibers for barrier membrane application.

    Science.gov (United States)

    Carter, Princeton; Rahman, Shekh M; Bhattarai, Narayan

    2016-01-01

    Guided tissue regeneration (GTR) is a widely used method in dental surgical procedures that utilizes a barrier membrane to exclude migration of epithelium and ensure repopulation of periodontal ligament cells at the sites having insufficient gingiva. Commercial GTR membranes are typically composed of synthetic polymers that have had mild clinical success mostly because of their lack of proper bioactivity and appropriate degradation profile. In this study, a natural polymer, aloe vera was blended with polycaprolactone (PCL) to create nanofibrous GTR membranes by electrospinning. Aloe vera has proven anti-inflammatory properties and enhances the regeneration of periodontium tissues. PCL, a synthetic polymer, is well known to produce miscible polyblends nanofibers with natural polymers. Nanofibrous membranes with varying composition of PCL to aloe vera were fabricated, and several physicochemical and biological properties, such as fiber morphology, wettability, chemical structure, mechanical strength, and cellular compatibility of the membranes were analyzed. PCL/aloe vera membranes with ratios from 100/00 to 70/30 showed good uniformity in fiber morphology and suitable mechanical properties, and retained the integrity of their fibrous structure in aqueous solutions. Experimental results, using cell viability assay and cell attachment observation, showed that the nanofibrous membranes support 3T3 cell viability and could be a potential candidate for GTR therapy.

  7. Evaluation of antimicrobial efficacy of Aloe vera and its effectiveness in decontaminating gutta percha cones

    Directory of Open Access Journals (Sweden)

    Prakash P Athiban

    2012-01-01

    Results: The zones of inhibition on the agar plate were measured as 24mm,21mm and 24mm respectively. The broth remained clear even after 48 hours of incubation. Conclusion: We conclude that Aloe vera is indeed effective as a GP decontaminant and it holds a promising future as a medium for storage of GP cones.

  8. In vivo tracing of organophosphorus pesticides in cabbage (Brassica parachinensis) and aloe (Barbadensis)

    Energy Technology Data Exchange (ETDEWEB)

    Qiu, Junlang; Chen, Guosheng; Zhou, Hong; Xu, Jianqiao; Wang, Fuxin; Zhu, Fang, E-mail: ceszf@mail.sysu.edu.cn; Ouyang, Gangfeng, E-mail: cesoygf@mail.sysu.edu.cn

    2016-04-15

    In vivo solid-phase microextraction (SPME) sampling method coupled with gas chromatography–mass spectrometry (GC–MS) analysis was employed to trace the uptake and elimination of organophosphorus pesticides (OPPs) in two kinds of edible plants, cabbage (Brassica parachinensis) and aloe (Barbadensis). The metabolism of fenthion in aloe was also investigated by the liquid chromatography tandem mass spectrometry analysis (LC–MS/MS) to understand the fate of OPPs in living plants better. Transpiration stream concentration factor (TSCF) and depuration rate constants of the OPPs in living plants were obtained therein. The health risk of the OPPs treated aloe was estimated by the maximum residue limit (MRL) approach, and it revealed that the OPPs were rather safe for their fast degradable property. However, peak concentration of fenthion-sulfoxide was found to exceed the MRL and was higher than that of the parent fenthion, which indicated the potential risk of pesticide metabolites. This study highlighted the application of in vivo SPME for contaminant tracing in different living edible plants. The in vivo tracing method is very convenient and can provide more data to evaluate the risk of different pesticides, which are very important for the safety of agriculture production. - Highlights: • In vivo SPME was employed to sample organophosphorus pesticides in vegetables. • Uptake and elimination of OPPs were traced in cabbage and aloe. • In vivo tracing of fenthion demonstrated its metabolites could be rather dangerous. • The risks of OPPs were assessed based on the in vivo tracing data.

  9. Standardization of micropropgation techniques for Aloe vera: a pharmaceutically important plant.

    Science.gov (United States)

    Zakia, Shehnaz; Zahid, Najma Yousaf; Yaseen, Mehwish; Abbasi, Nadeem Akhtar; Hafiz, Ashfaq Ahmad; Mahmood, Nasir

    2013-11-01

    Aloe vera (Syn Aloe barbadensis Mill.), a medicinal plant, has a great potential in cosmetic and drug industry due to presence of more than 200 bioactive compounds. Natural propagation of Aloe vera, by means of suckers, is very slow and insufficient to meet the increasing demand of pharmaceutical and cosmetic industries. Shoot tip was used as an explant for in vitro regeneration of Aloe vera. Explants were disinfested with the use of 0.1% mercuric chloride and 0.5% sodium hypochlorite, and washed thoroughly with autoclaved distilled water. Solid MS medium was used with addition of different concentrations of 6-benzyl aminopurine and α-naphthalene acitic acid. After 7 weeks of inoculation, greatest number of shoots (11.18) and highest shoot length (12.15cm) were found in MS medium supplemented with 0.5 mg l-1 6-benzylaminopurine (BAP) along with same concentration of α-naphthalene acitic acid (NAA). Best rooting (84.67%) was found in medium supplemented with 1.5 mg l-1 of indole butyric acid (IBA). The rooted explants were then gradually acclimatized and shifted to green house.

  10. Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens.

    Science.gov (United States)

    Akhtar, Masood; Hai, Abdul; Awais, Mian Muhammad; Iqbal, Zafar; Muhammad, Faqir; ul Haq, Ahsan; Anwar, Muhammad Irfan

    2012-05-25

    This paper reports the immunostimulatory and protective effects of Aloe vera extracts (aqueous and ethanolic) against coccidiosis in industrial broiler chickens. The study was divided into two experiments. Experiment-I was conducted for the evaluation of immunostimulatory activity of A. vera and experiment-II demonstrated the protective efficacy of A. vera extracts against coccidiosis in chickens. Results of the experiment-I revealed significantly higher (pvera as compared to those administered with aqueous extract and control group. Microplate haemagglutination assay for humoral response on day 7th and 14th post primary and secondary injections of sheep red blood cells (SRBCs) revealed significantly higher (pAloe extract as compared to the ethanolic extract administered chickens (45%). Mean oocysts per gram of droppings in the control group was significantly higher (pAloe extract showed a minimal mean lesion score (2.3) followed by those administered with ethanolic Aloe extract (2.6) and control chickens (3.05) for caeca, and a similar pattern was observed for intestinal lesion scoring. Further, significantly higher weight gains and antibody titers (pvera extracts as compared to those in the control group. It was concluded that A. vera may be a potential and valuable candidate to stimulate the immune responses and can be used successfully as an immunotherapeutic agent against coccidiosis in industrial broiler chickens.

  11. A Systemic Review on Aloe arborescens Pharmacological Profile: Biological Activities and Pilot Clinical Trials.

    Science.gov (United States)

    Singab, Abdel-Naser B; El-Hefnawy, Hala M; Esmat, Ahmed; Gad, Haidy A; Nazeam, Jilan A

    2015-12-01

    Since ancient times, plants and herbal preparations have been used as medicine. Research carried out in the last few decades has verified several such claims. Aloe arborescens Miller, belonging to the Aloe genus (Family Asphodelaceae), is one of the main varieties of Aloe used worldwide. The popularity of the plant in traditional medicine for several ailments (antitumor, immunomodulatory, antiinflammatory, antiulcer, antimicrobial and antifungal activity) focused the investigator's interest on this plant. Most importantly, the reported studies have shown the plant effectiveness on various cancer types such as liver, colon, duodenal, skin, pancreatic, intestinal, lung and kidney types. These multiple biological actions make Aloe an important resource for developing new natural therapies. However, the biological activities of isolated compounds such as glycoprotein, polysaccharides, enzyme and phenolics were insufficient. Considering all these, this contribution provides a systematic review outlining the evidence on the biological efficacy of the plant including the pharmacology and the related mechanisms of action, with specific attention to the various safety precautions, and preclinical and clinical studies, indicating the future research prospects of this plant.

  12. Caracterización e identificación de aislados de levaduras carotenogénicas de varias zonas naturales del Ecuador

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    Javier Carvajal-Barriga

    2009-12-01

    Full Text Available Characterization and identification of isolates of carotenogenic yeast strains from several natural zones of Ecuador. Objective. Toidentify and characterize pigmented yeasts isolated from natural environments in Ecuador, producing metabolites with industrial importanceand with potential use in further biotechnological applications. Materials and methods. Twenty-six pigmented isolates from the “Colecciónde Levaduras Quito-Católica” (CLQCA were exposed to physiological and molecular tests for their characterization, typification andidentification. Sugar assimilation ability was evaluated. Molecular techniques such as ITS-RFLP, MSP-PCR, partial amplification of thesegment 26S of the rDNA, and sequencing were used for their identification at the species level. Growth curves for each isolate accordingto their specific optimum growth temperatures. Results. The following species were identified: Rhodotorula mucilaginosa, Rhodosporidiumbabjevae, Sporidiobolus ruineniae, Rhodotorula glutinis, Rhodotorula slooffiae, as well as a new species of the genus Rhodotorula.Conclusions. Rhodotorula mucilaginosa (CLQCA-12-013 and CLQCA-12-008 and Rhodosporidium babjevae (CLQCA-10-188isolates were selected as promising strains for industrial applications, because they showed characteristics such as rapid growth, highassimilation of sugars, biomass production, and pigment synthesis.

  13. Invisible, luego aislado. Efectos comparativos de la virtualidad del equipo y de la tarea en el aislamiento en el trabajo y los resultados laborales

    Directory of Open Access Journals (Sweden)

    Mehmet A. Orhan

    2016-06-01

    Full Text Available The new standards of technological and flexible arrangements have made virtual work prevalent for almost everyone in all levels of an organization. Whether in a virtual or traditional setting, current working conditions allow anyone to collaborate, work and interact with others through electronic means of communication, thereby creating a lack of face-to-face contact. Even though the dynamics of virtuality have been widely elaborated at the team level, there are still many unknowns about the impacts of virtuality experienced at an individual level. This paper aims to shed light on the relationship between workplace social isolation, job satisfaction, perceived performance and turnover intention comparing individual responses to team virtuality and task virtuality. Our findings propose that there are statistically significant relations between individual task virtuality, workplace social isolation, satisfaction, perceived performance and turnover intention in organizations. The results also reveal that task virtuality is a better predictor than team virtuality in estimating workplace social isolation and turnover intention.

  14. Variabilidad clonal de la polimerasa NS5b del virus de la hepatitis C en aislados de muestras clínicas

    OpenAIRE

    Salvatierra, Karina Alejandra

    2010-01-01

    La infección crónica provocada por el virus de la hepatitis C (VHC) sigue siendo un gran problema de salud pública a nivel mundial. Se estima que 200 millones de personas (3% de la población mundial) se encuentran infectadas por este virus.El tratamiento actual basado en la combinación de dos fármacos no específicos, el interferón-α pegilado y la ribavirina; provoca numerosos efectos secundarios, el tratamiento es de larga duración y además es costoso y tiene poco éxito en pacientes infectado...

  15. Aislamiento y caracterización de microorganismos con actividad lipolítica provenientes de sedimentos del Humedal El Jaboque (Bogotá, Colombia

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    Valencia Zapata Hernando

    2006-12-01

    Full Text Available En el humedal el Jaboque (Engativá Bogotá, Colombia, se aislaron microorganismos lipolíticos provenientes de
    sedimentos, mediante la utilización de medios de cultivo selectivos (agar tributirina, agar aceite de girasol. Primero se evaluó la concentración del sustrato (aceite de girasol en la cual se encontraba el mayor número de morfotipos aislados, luego se comparó la eficiencia de los dos medios de cultivo de acuerdo a la cantidad de morfotipos aislados. Se evaluaron tres concentraciones diferentes de sustrato. Se aisló un total de 18 cepas pertenecientes a los géneros Bacillus, Pseudomonas, Acinetobacter, Streptomyces, Penicillium, Alternaria. Los medios evaluados constituyen alternativas sencillas de aislamiento de microorganismos con actividad lipolítica en sedimentos.

  16. Medios de cultivo líquidos: un avance para la micropropagación comercial de zábila (Aloe barbadensis Mill.

    Directory of Open Access Journals (Sweden)

    Nilca Rosa Albany

    2015-05-01

    Full Text Available Título en imgles: Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill. Título corto: Un avance para la micropropagación comercial de zábilaResumen: La micropropagación es una alternativa para la producción comercial de plantas de zábila (Aloe barbadensis Mill. limitada por los altos costos de producción. Con el objetivo de prescindir de los agentes gelificantes, reduciendo costos, se comparó el medio de cultivo líquido con el medio de cultivo gelificado en las diferentes etapas de micropropagación de la zábila. En la etapa de establecimiento se observó mayor porcentaje de explantes contaminados en el medio de cultivo líquido estático (25.55% que en el medio gelificado (11.11%; y aunque el resto de los explantes se establecieron independientemente de la condición del medio de cultivo, en el medio líquido alcanzaron mayor altura (3.81 cm que en el medio gelificado (3.03 cm. En la etapa de multiplicación, la altura de los explantes (entre 4.43 y 6.01 cm fue superior en los recipientes de inmersión temporal automatizado (RITA® en comparación con el medio gelificado (entre 3.24 y 3.42 cm; sin diferencias significativas entre el número de brotes/explante. Todos los brotes enraizaron a los 30 días independientemente del medio de cultivo empleado (líquido estático y gelificado, sin observar variaciones en la altura del brote y, número y longitud de las raíces. El empleo de los medios de cultivo líquidos y la implementación de los sistemas de inmersión temporal tipo RITA® permiten reducir los costos de producción al prescindir de los agentes gelificantes, lo que representa un avance para la micropropagación comercial de zábila. Palabras clave: Cultivo de tejidos, agentes gelificantes, RITA®, sistemas de inmersión temporal.Abstract: Micropropagation is considered a successful alternative for aloe (Aloe barbadensis Mill. plant production. However, it has limited use

  17. Resistencia a antibioticos y presencia de plasmidos en: Aeromonas hydrophila, Vibrio fluvialis y Vibrio furnissii, aislados de Carassius auratus auratus

    OpenAIRE

    Pilar Negrete Redondo; Jorge Romero Jarero; José Luis Arredondo Figueroa

    2004-01-01

    Se aislaron e identificaron 70 cepas bacterianas del riñón de peces de ornato Carassius auratus con signos y lesiones de infección. Las especies bacterianas aisladas e identificadas, Aeromonas hydrophila, Vibrio fluvialis y Vibrio furnissii, han sido descritas como causantes de diarrea en humano y como patógeno muy agresivo en cultivos de Ciprinus carpio. Por esta razón se ha hecho mal uso y abuso de antibióticos para evitar y controlar la presencia de esta bacteria en los cultivos. El uso fu...

  18. Caracterización por ardrea de microorganismos acidófilos aislados en minas de oro de marmato, colombia

    OpenAIRE

    Márquez F., Edna Judith; MÁRQUEZ G., MARCO; Osorio E., Victor Manuel

    2008-01-01

    La bio-oxidación de minerales mejora la recuperación de metales valiosos y disminuye el impacto negativo causado por los subproductos de las operaciones mineras, pero las interacciones de los microorganismos involucrados son poco conocidas. Con el objeto de avanzar en el estudio de interacciones microbianas de bacterias acidófilas nativas en cultivos mixtos, en este trabajo se utilizó la siembra en medio sólido doble capa y el análisis de enzimas de restricción del ADN ribosomal amplificado (...

  19. RESPUESTA ANTAGÓNICA DE MICROORGANISMOS AISLADOS DE LOS CULTIVOS DE MICROALGAS EN EL LABORATORIO DE CULTIVOS MARINOS

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    Carmen Ruíz Huamán

    2013-10-01

    Full Text Available Durante el 2012, el Laboratorio de Cultivos Marinos obtuvo bacterias aisladas de los cultivos de microalgas. Se trabajó 60 colonias de los cultivos. Se confrontó una a una las colonias aisladas del cultivo de microalgas con las bacterias patógenas tipo ATCC, estas fueron: Flavobacterium psychrophilum, Aeromona hydrophila, Lactococcus garviaceae y Vibrio herveyi. Solo una bacteria tuvo interacción antagónica ya que formó el halo de inhibición.

  20. Mapping Sargassum beds off, ChonBuri Province, Thailand, using ALOS AVNI2 image

    Science.gov (United States)

    Noiraksar, Thidarat; Komatsu, Teruhisa; Sawayama, Shuhei; Phauk, Sophany; Hayashizaki, Ken-ichi

    2012-10-01

    Sargassum species grow on rocks and dead corals and form dense seaweed beds. Sargassum beds play ecological roles such as CO2 uptake and O2 production through photosynthesis, spawning and nursery grounds of fish, feeding ground for sea urchins and abalones, and substrates for attached animals and plants on leaves and holdfasts. However, increasing human impacts and climate change decrease or degrade Sargassum beds in ASEAN countries. It is necessary to grasp present spatial distributions of this habitat. Thailand, especially its coastal zone along the Gulf of Thailand, is facing degradation of Sargassum beds due to increase in industries and population. JAXA launched non-commercial satellite, ALOS, providing multiband images with ultra-high spatial resolution optical sensors (10 m), AVNIR2. Unfortunately, ALOS has terminated its mission in April 2011. However, JAXA has archived ALOS AVNIR2 images over the world. They are still useful for mapping coastal ecosystems. We examined capability of remote sensing with ALOS AVNIR2 to map Sargassum beds in waters off Sattahip protected area as a natural park in Chon Buri Province, Thailand, threatened by degradation of water quality due to above-mentioned impacts. Ground truth data were obtained in February 2012 by using continual pictures taken by manta tow. Supervised classification could detect Sargassum beds off Sattahip at about 70% user accuracy. It is estimated that error is caused by mixel effect of bottom substrates in a pixel with 10 x 10 m. Our results indicate that ALOS AVNIR2 images are useful for mapping Sargassum beds in Southeast Asia.

  1. Pengaruh Lidah Buaya (Aloe vera L. pada Paru-Paru Tikus yang Diinduksi Asap Rokok

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    Nur Atik

    2012-09-01

    Full Text Available The incident of smoking is one of the world’s health problems, as smoking increases the incidence of chronic obstructive pulmonary disease (COPD. Aloe vera L has anti-inflammatory, immunomodulator, and β-sitosterol properties. A research was conducted to observe its effects in preventing the cell apoptotic events as well as the increasing numbers of macrophages and the decreasing phagocytotic function of macrophages in rat’s lung (Rattus norvegicus that has been induced by cigarettes smoke. The study was conducted in January 2009–January 2010, located in Department of Cell Biology, Clinical Pathology, Anatomy Pathology and Health Research Unit of Faculty of Medicine University of Padjadjaran.This experimental study was conducted on rats which were divided into 3 groups each consisted 8 rats for 42 days in treatments. The data were collected from number of macrophages and its activity and also alveoli epithelial cell Bcl2 expression observation, than statistical analytic was performed using Mann-Whitney test. Result showed the significant difference (p<0.05 in number of macrophages, cigarette smoke group had a higher number (495.88±104.09 than cigarette smoke plus aloe group (295.63±176.79 but it showed the opposite for the phagocytotic function of macrophages. The cigarette smoke plus aloe group had higher percentage (11.67±12.84 than cigarette smoke group (3.77±1.39. Observation of Bcl2 expression in rat’s lungs alveoli cell showed that the group exposed to cigarettes and Aloe vera were more expressed compared with cigarette exposed group (p <0.05. This study proved that Aloe vera gel increases the number and cell activity of macrophages in addition to the Bcl2 expression in rat lungs which have been induced by cigarette smoke.

  2. STANDWISE CHANGE DETECTION FOR GROWING STOCK USING REPEAT-PASS ALOS PALSAR / PALSAR-2 DATA

    Directory of Open Access Journals (Sweden)

    M. –G. Hong

    2016-06-01

    Full Text Available This study demonstrates the possibility of detecting the changes of growing stocks in mountainous forest stands derived from ALOS PALSAR and PALSAR-2 images. The ALOS PALSAR were obtained over the Kwangneung Experiment Forest (KEF, Korea during the period of nineteen and a half months from the April 26, 2009 to December 12, 2010, whereas the PALSAR-2 data were acquired on the April 7, 2015. The KEF test site comprises 58 stands, which cover approximately 1,000ha and have steep slope topography. Owing to topographic effects of SAR data in mountainous areas, the DEM-assisted topographic normalized backscattering coefficient γ0 was applied to the evaluation of the relationships between the ALOS PALSAR / PALSAR-2 HV backscatter and the field inventory–based stand stock volume. The results indicate that: 1 the γ0 values for the volume obtained from ALOS PALSAR data on December 12, 2010 show a gradual increase higher than those computed from the data on April 26, 2009, here the γ0 value increases in accordance with an increase in the volume: 2 the γ0 values determined from the PALSAR-2 data increase with the same inventory-based volume, when compared with those computed from both ALOS PALSAR data. They also increase substantially as the values of the volume rise, with the exception of the volume interval from 130 m3 ha−1 to 160 m3 ha−1. This is understandable because the volume of the aforementioned interval has been reduced through clearing. Consequently, the γ0–based relationship between PALSAR-2 HV backscatter and growing stock can lead to detecting the stand growth changes in the KEF of Korea.

  3. Diffusion of hydroxyl ions from calcium hydroxide and Aloe vera pastes.

    Science.gov (United States)

    Batista, Victor Eduardo de Souza; Olian, Douglas Dáquila; Mori, Graziela Garrido

    2014-01-01

    This study evaluated the diffusion through the dentinal tubules of hydroxyl ions from different calcium hydroxide (CH) pastes containing Aloe vera. Sixty single-rooted bovine teeth were used. The tooth crowns were removed, the root canals were instrumented and the specimens were assigned to 4 groups (n=15) according to the intracanal medication: Group CH/S - CH powder and saline paste; Group CH/P - CH powder and propylene glycol paste; Group CH/A - calcium hydroxide powder and Aloe vera gel paste; Group CH/A/P - CH powder, Aloe vera powder and propylene glycol paste. After placement of the root canal dressings, the teeth were sealed coronally and apically with a two-step epoxy adhesive. The teeth were placed in identified flasks containing deionized water and stored in an oven with 100% humidity at 37 °C. After 3 h, 24 h, 72 h, 7 days, 15 days and 30 days, the deionized water in the flasks was collected and its pH was measured by a pH meter. The obtained data were subjected to statistical analysis at a significance level of 5%. The results demonstrated that all pastes provided diffusion of hydroxyl ions through the dentinal tubules. The combination of Aloe vera and CH (group CH/A) provided a constant release of calcium ions. Group CH/A/P showed the highest pH at 24 and 72 h. In conclusion, the experimental pastes containing Aloe vera were able to enable the diffusion of hydroxyl ions through the dentinal tubules.

  4. In-vitro assessment and pharmacodynamics of nimesulide incorporated Aloe vera transemulgel.

    Science.gov (United States)

    Vandana, K R; Yalavarthi, Prasanna R; Sundaresan, C R; Sriramaneni, Raghava N; Vadlamudi, Harini C

    2014-06-01

    The aim of the investigation was to prepare nimesulide emulsion for incorporation in Aloe vera gel base to formulate 'nimesulide - Aloe vera transemulgel' (NAE) and to carryout in-vitro assessment and in-vivo anti-inflammatory studies of the product. Although the use of nimesulide is banned for oral administration, due to its potential for inducing hepatotoxicity and thrombocytopenia, the use of nimesulide for topical delivery is prominent in the treatment of many inflammatory conditions including rheumatoid arthritis. The drug loading capacity of transdermal gels is low for hydrophobic drugs such as nimesulide. Nimesulide can be effectively incorporated into emulgels (a combination of emulsion and gel). Aloe vera has a mild anti-inflammatory effect and in the present study Aloe vera gel was formulated and used as a gel base to prepare NAE. The emulgels thus prepared were evaluated for viscosity, pH, in-vitro permeation, stability and skin irritation test. In-vivo anti-inflammatory studies were performed using carrageenan induced hind paw edema method in Wistar rats. The results were compared with that of commercial nimesulide gel (CNG). From the in-vitro studies, effective permeation of nimesulide from NAE (53.04 %) was observed compared to CNG (44.72 %) at 30 min indicating better drug release from NAE. Topical application of the emulgel found no skin irritation. Stability studies proved the integrity of the formulation. The percentage of inhibition of edema was highest for the prepared NAE (67.4 % inhibition after 240 min) compared to CNG (59.6 %). From our results, it was concluded that the Aloe vera gel acts as an effective gel base to prepare nimesulide emulgel with high drug loading capacity (86.4 % drug content) compared to CNG (70.5 % drug content) with significant anti-inflammatory effect.

  5. 芦荟浸泡酒的工艺研究%Technology of aloe steeping wine

    Institute of Scientific and Technical Information of China (English)

    何林; 刘晓娟; 赵力超; 刘欣

    2012-01-01

    以库拉索芦荟为原料,以浓香型的蒸馏白酒为基酒,对芦荟浸泡酒的工艺进行了研究。在单因素实验基础上,利用正交实验研究芦荟添加量、超声波萃取时间、陈酿时间对芦荟浸泡酒感官品质和芦荟多糖含量的影响以确定最佳工艺条件,并进行了后期调味实验。结果表明:芦荟添加量90 g/100 mL,超声波萃取时间10 min,陈酿时间15 d,甜菊糖苷添加量0.08 g/100 mL时,得到的芦荟浸泡酒品质最佳。%Aloe vera was used as raw material and strong aromatic Chinese spirits was used as base liquor. The technology of aloe steeping wine was studied. On the base of single factor test, in order to obtain the optional technology conditions, orthogonal tests were done. The influence of addition of aloe, ultrasonic extraction time and aging time on thesensory quality and polysaccharide content was studied. At the later stage, the seasoning experiment was done. The results showed that: the addition of aloe 90 g/100 mL, the ultrasonic extraction time 10 min, the aging time 15 d, the addition of of stevioside 0.08 g/100 mL, the quality of aloe steeping wine was best.

  6. Case Reports of Bedsores Using Aloe Vera Gel Powder with High Molecular Weight

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    Keizo Matsuo

    2009-01-01

    Full Text Available It has been reported that the Japanese have higher rate of life expectancy and that Japan is rapidly becoming a super aging society. Almost 5 per cent of the aged, who develop diseases such as cerebrovascular related disorders, mental problems, fractures, cancer and infections also end up suffering from bedsores. It has been found that patients often opt for treatment, when the bedsores become very severe and adding more complications in their treatment. It may be presumed that prevention and early detection are vital for the treatment of bed sores. In our present study, we have tested herbal formulations having Aloe vera gel powder for its efficacy and activity on bed sores. Aloe vera gel powder with high molecular weight (AHM was prepared from the gel part, by washing with running water using the patented freeze-drying under micro wave and far infra red irradiations in which barbaloin content was less than 10 ppm in powder form. The treatment was given by applying the macromolecule aloe ointment for bedsores from I degree to Il degree ulcer patients. The results have shown that AHM in the ointment form indicated a high possibility to cure bedsores. Being very difficult to cure, due to the patient′s peculiar conditions such as old age, inability of the patient to turn by himself/herself and also due to complications caused by other symptoms. We were able to confirm the effectiveness of the macromolecule aloe ointment in four cases of bedsores with two cases of positive control, using the Design Score and by checking the side effects. In this study, our report is based on the preclinical trials for bedsores by the external use of the macromolecule aloe ointment.

  7. Clinical Effectiveness of Aloe Vera in the Management of Oral Mucosal Diseases- A Systematic Review

    Science.gov (United States)

    Nair, Gopakumar Ramachandran; Naidu, Giridhar Seetharam; Jain, Supreet; Makkad, Ramanpal Singh; Jha, Abhishek

    2016-01-01

    Introduction Aloe vera is well known for its medicinal properties which lead to its application in treating various diseases. Its use in treating oral lesions has not been much documented in literature. Aim Although, systematic reviews on aloe vera and its extracts have been done earlier, but in relation to oral diseases this is the first systematic review. The aim of the present systematic review was to compile evidence based studies on the effectiveness of Aloe vera in treatment of various oral diseases. Materials and Methods Computerized literature searches were performed to identify all published articles in the subject. The following databases were used: PUBMED [MEDLINE], SCOPUS, COCHRANE DATABASE, EMBASE and SCIENCE DIRECT using specific keywords. The search was limited to articles published in English or with an English Abstract. All articles (or abstracts if available as abstracts) were read in full. Data were extracted in a predefined fashion. Assessment was done using Jadad score. Results Fifteen studies satisfied the inclusion criteria. Population of sample study ranged from 20 patients to 110 patients with clinically diagnosed oral mucosal lesions. Out of 15 studies, five were on patients with oral lichen planus, two on patients with oral submucous fibrosis, other studies were carried on patients with burning mouth syndrome, radiation induced mucositis, candida associated denture stomatitis, xerostomic patients and four were on minor recurrent apthous stomatitis. Most studies showed statistically significant result demonstrating the effectiveness of Aloe vera in treatment of oral diseases. Conclusion Although there are promising results but in future, more controlled clinical trials are required to prove the effectiveness of Aloe vera for management of oral diseases. PMID:27656587

  8. The increasing of fibroblast growth factor 2, osteocalcin, and osteoblast due to the induction of the combination of Aloe vera and 2% xenograft concelous bovine

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2012-12-01

    Full Text Available Background: To make a successfull denture prominent ridge is needed, preservation on tooth extraction socket is needed in order to prevent alveol bone resorption caused by revocation trauma. An innovative modification of the material empirically suspected to be able reduce inflammation caused by the revocation trauma is a combination of Aloe vera and xenograft concelous bovine (XCB and Aloe vera is a biogenic stimulator and accelerating the growth of alveolar ridge bone after tooth extraction. Purpose: The research was aimed to determine of the increasing alveol bone formation by inducing the combination of Aloe vera and 2% xenograft concelous bovine. Methods: To address the problems, the combination of Aloe vera and xenograft concelous bovine was induced into the tooth extraction sockets of Cavia cabayas which devided on 8 groups. Groups control, filled with XCB, Aloe vera and Aloe vera and XCB combination, at 7 days and 30 days after extraction. Afterwards, immunohistochemical examination was conducted to examine the expressions of FGF-2 and osteocalcin, as the product of the growth of osteoblasts. Results: There were significantly increases expression of FGF-2 and osteocalcyn on group which filled with XCB, Aloe vera and combined Aloe vera and XCB. Conclusion: It may be concluded that the induction of the combination of Aloe vera and xenograft concelous bovine into the tooth sockets can enhance the growth expressions of FGF-2 and osteocalcin as the product of osteoblasts, thus, the growth of alveolar bone was increased.Latar belakang: Untuk keberhasilan pembuatan gigitiruan diperlukan ridge yang prominent, maka diperlukan suatu preservasi soket pencabutan gigi untuk mencegah terjadinya resopsi tulang alveolar akibat trauma pencabutan. Suatu inovasi modifikasi bahan yang diduga secara empiris dapat mengurangi keradangan karena trauma pencabutan adalah berupa kombinasi Aloe vera dan xenograft concelous bovine (XCB. Aloe vera yang merupakan

  9. Ultraestructura del espermatozoide de rata (Ratus norvegicus). Aplicación de tres técnicas para Microscopía Electrónica de Transmisión

    OpenAIRE

    Ureña, Francisco; Akahori, Hiroshi; Azofeifa, Alfredo

    2016-01-01

    Se hizo un estudio ultraestructural del espermatozoide de rata tomado del tubo seminífero, con microscopia electrónica de transmisión y mediante la aplicación de las técnicas de: secciones finas, microextendidos y réplicas. Los espermatozoides aislados y extendidos, presentan una cabeza de gran densidad electrónica, muy homogénea y ligeramente curva en el extremo anterior; las secciones finas y longitudinales de esta zona corroboran lo compacto y homogéneo del ADN, cubierto por las membranas ...

  10. Actinomycetes bioactivos de sedimento marino de la costa central del Perú

    OpenAIRE

    Jorge León; Libia Liza; Isela Soto; D´Lourdes Cuadra; Lilian Patiño; Rito Zerpa

    2013-01-01

    En el presente trabajo evaluamos la actividad antibacteriana y antifúngica de actinomycetes marinos sobre patógenos de origen clínico. Asimismo, fueron evaluadas la capacidad de producir enzimas extracelulares como carbohidrasas, lipasas y proteasas. Los Actinomycetes fueron aislados de sedimentos colectados entre setiembre a diciembre del 2005 de las Bahías de Ancón (Lima) e Independencia (Ica) de 34 y 100 m de profundidad. El aislamiento se realizó en Agar Caseína - Almidón (ACA) y Agar Mar...

  11. Efecto del ayuno sobre la absorción de Ciprofloxacina : Modelo in vitro

    OpenAIRE

    Volonté, María Guillermina

    1993-01-01

    Se analiza el efecto del ayuno sobre la absorción gastrointestinal de ciprofloxacina mediante la utilización de un modelo in vitro que emplea estómagos y segmentos intestinales proximales aislados de ratas, separadas en dos lotes, uno de ellos sometido a un ayuno de 16 horas y el otro liberado a una dieta de alimento balanceado a voluntad. Los resultados muestran una transferencia intestinal parcial de la droga y una absorción estomacal despreciable, comprobándose que el ayuno no tiene efecto...

  12. Evaluación de hongos y bacterias aislados de gallinaza en el biocontrol de Sclerotium cepivorum Berk

    Directory of Open Access Journals (Sweden)

    Gladys Amparo Sarmiento

    2013-07-01

    Full Text Available Objetivo. Seleccionar hongos y bacterias de la gallinaza por su capacidad antagónica en el biocontrol de S. cepivorum in vitro y en invernadero. Materiales y métodos. De dos fuentes de gallinaza, una pura y otra compuesta, se tomaron sendas muestras de 100 g, se colocaron en erlemeyers, se agregó agua destilada estéril hasta completar un litro y se agitó durante 5 minutos. A partir de esta suspensión se prepararon diluciones de 1x10-1 hasta 1x10-4, y se evaluó el crecimiento y esporulación de S. cepivorum. Los microorganismos seleccionados in vitro fueron evaluados en el control de la enfermedad en invernadero. Se evaluó la incidencia de la enfermedad y el porcentaje de plantas muertas. Resultados. De las dos fuentes de gallinaza se aislaron 13 colonias de hongos y 26 de bacterias, para un total de 39 aislamientos, de los cuales, tras ser evaluados in vitro, fueron seleccionados tres aislamientos de hongos (H2, H5, H6 y una bacteria (B21 por su capacidad antagónica a S. cepivorum, a los 6, 9, 12 y 15 días, y la producción de esclerocios, con diferencias altamente significativas en comparación con el testigo. Los aislamientos H2 y H6 corresponden al género Trichoderma; el H5, a Penicillium, y B21, a Bacillus. Con H2, H5, H6 y B21, los síntomas de la enfermedad fueron observados en la semana 13, con una incidencia del 20%, y en el testigo, en la semana novena, con una incidencia del 70%, y 40% de muerte de plántulas. Conclusión. Las gallinazas son una fuente de microorganismos biocontroladores de S. cepivorum.

  13. Evaluation of radioprotective effect of aloe vera and zinc/copper compounds against salivary dysfunction in irradiated rats.

    Science.gov (United States)

    Nejaim, Yuri; I V Silva, Amaro; V Vasconcelos, Taruska; J N L Silva, Emmanuel; M de Almeida, Solange

    2014-09-01

    The aim of this study was to evaluate the radioprotective and reparative effects of compounds based on aloe vera, zinc, and copper against salivary gland dysfunction in Wistar rats. A total of 150 Wistar rats were randomly divided into 12 groups, in which the animals received aloe vera and/or zinc and copper. In eight of these groups the animals were also subjected to irradiation before or after administration of the substances. After 27 days, sialometry tests were performed. Data were analyzed using ANOVA and the Tukey test (P aloe vera before or after irradiation showed a significantly higher salivary flow rate than rats that had been simply irradiated. When both substances were administered, a statistically significant difference in the salivary flow rate was observed in comparison with the irradiation alone group seven days after irradiation. The present results suggest that aloe vera exerts positive protective and reparative effects, and can be considered a potential radioprotective substance.

  14. Uskumatult põnev maailm läbi Rein Marani kaamerasilma / Rein Maran ; üles kirjutanud Alo Lõhmus

    Index Scriptorium Estoniae

    Maran, Rein, 1931-

    2011-01-01

    Valitud katkendeid Alo Lõhmuse koostatud peatselt ilmuvast Rein Marani filmilooraamatust "Ööbikut ei tohi reeta". Rein Maran tähistab 13. septembril 80. sünnipäeva. Andmeid Rein Marani elukäigu kohta

  15. A Comparison between Antibacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an In Vitro Study)

    OpenAIRE

    Ehsani, Maryam; Amin Marashi, Mahmood; Zabihi, Ebrahim; Issazadeh, Maryam; Khafri, Soraya

    2013-01-01

    Removing the bacteria, including Enterococcus faecalis, from the root canal is one of the important aims in endodontic treatment.We aimed to compare the antibacterial activity of Chlorhexidine with two natural drugs. The antibacterial activities of three different propolis extracts (alcohol concentrations: 0, 15, 40%) and Aloe vera gel on E. faecalis were compared using three methods: disk diffusion, microdilution and direct contact test. In addition to the above bacterium, the Aloe vera gel ...

  16. Effects of different levels of Aloe vera gel as an alternative to antibiotic on performance and ileum morphology in broilers

    Directory of Open Access Journals (Sweden)

    Ali Mahdavi

    2011-07-01

    Full Text Available The present study attempts to analyze the effects of different levels of Aloe vera gel as an alternative to antibiotic, on performance and ileum morphology in broilers. Three hundred one-day old Ross 308 male broilers were used on a completely randomized design in 5 groups with 4 replicates, each consisting of 15 broilers. The groups included the control group (basal diet and three groups with basal diet mixed with different levels of Aloe vera gel (1.5%, 2% and 2.5%. Finally, there was a group with basal diet plus 15 ppm antibiotic virginiamycin. The results obtained regarding performance of the broilers showed that Aloe vera gel groups brought about higher body weight gain and feed intake compared to the control group; however, no significant difference was observed in feed conversion ratio between the groups treated by Aloe vera gel and the control group (P>0.05. Although the antibiotic group showed better performance and heavier dressing percentage than the Aloe vera gel and the control groups, no significant difference was seen between the group treated by 2% Aloe vera gel and the antibiotic group regarding body weight gain, feed conversion ratio and dressing percentage (P>0.05. Among the Aloe vera gel groups, the 2% Aloe vera gel group had the largest villus height and the greatest villus height to crypt depth ratio compared to the antibiotic group (PAloe vera gel treatment may be recommended to achieve the best performance in broilers as an alternative to antibiotic growth promoter.

  17. A novel method for air drying aloe leaf slices by covering with filter papers as a shrink-proof layer.

    Science.gov (United States)

    Kim, S A; Baek, J H; Lee, S J; Choi, S Y; Hur, W; Lee, S Y

    2009-01-01

    To prevent the shrinkage of aloe vera slices during air drying, a method utilizing a shrink-proof layer was developed. The sample was configured of whole leaf aloe slices, where 1 side or both sides were covered with filter papers as shrink-proof layers. After air drying by varying the air temperature and the slice thickness, the drying characteristics, as well as several quality factors of the dried aloe vera leaf slices, were analyzed. In the simulation of the drying curves, the modified Page model showed the best fitness, representing a diffusion-controlled drying mechanism. Nonetheless, there was a trace of a constant-rate drying period in the samples dried by the method. Shrinkage was greatly reduced, and the rehydration ratios increased by approximately 50%. Scanning electron microscopic analysis revealed that the surface structure of original fibrous form was well sustained. FT-IR characteristics showed that the dried samples could sustain aloe polysaccharide acetylation. Furthermore, the functional properties of the dried slices including water holding capacity, swelling, and fat absorption capability were improved, and polysaccharide retention levels increased by 20% to 30%. Therefore, we concluded that application of shrink-proof layers on aloe slices provides a novel way to overcome the shrinkage problems commonly found in air drying, thereby improving their functional properties with less cost. Practical Application: This research article demonstrates a novel air drying method using shrink-proof layers to prevent the shrinkage of aloe slices. We analyzed extensively the characteristics of shrinkage mechanism and physical properties of aloe flesh gels in this drying system. We concluded that this method can be a beneficial means to retain the functional properties of dried aloe, and a potential alternative to freeze drying, which is still costly.

  18. Bioaktivitas Gel Aloe vera pada Gonad Tikus Putih Jantan (Rattus norvegicus

    Directory of Open Access Journals (Sweden)

    I Ketut Suardita

    2013-11-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE The purpose of this study was to determine Bioactivity of Aloe vera gel on gonad of male white rats (Rattus norvegicus. This research used complately random design, with three treatments namely T0, received placebo as a control; T1, received gel of Aloe vera 300 mg/kg body weight orally; and T2, received gel of Aloe vera 400 mg/kg body weight orally. Aloe vera gel was administrated every day for 21 days. All the treatments repeated three times, and each experimental unit used one white rat. Data found was analyzed using one way of Anova, and the Least Significant Differences (LSD Test was applied for further analysis. Results showed that Aloe vera gel administration with a dose of 300 mg/kg body weight had effected significantly on the number of spermatogonia cells, live-daed rasio of spermatozoa, and the percentage of sperm abnormality. The result of the research indicates that Aloe vera gel is potentially cytotoxic to testes cells, and so, it’has a high possibilities as an alternative herbal contraceptive agent for animals /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; text-align:justify; line-height:150%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;}

  19. Manejo nutricio del adulto con diabetes mellitus tipo 2

    OpenAIRE

    Claudia Hunot Alexander

    2001-01-01

    El manejo nutricio del adulto con diabetes tipo2, es la parte angular en el tratamiento de esta enfermedad. Es un proceso que requiere de unconocimiento profundo de nutrición y alimenta-ción, en donde el profesional de la salud recibeun entrenamiento a profundidad en esta áreacon la finalidad de transmitir esta información alos pacientes. El establecimiento de un plan decuidado nutricio, implementado a través del pro-ceso de terapia médico nutrimental, involucrael trabajo multidisciplinario d...

  20. DOCUMENTO: FORMAS DE EVALUACIÓN DEL APRENDIZAJE

    Directory of Open Access Journals (Sweden)

    Marcela Currea Galvis

    2004-08-01

    Full Text Available Las notas de seguimiento (usadas por casi todos los docentes y educadores se constituyen en lo que se conoce (en muchas instituciones cómo evaluaciones parciales y finales, y permiten utilizar diferentes tipos y esquemas para laevaluación del aprendizaje y del desarrollo de competencias, es decir, la articulacióny uso de saberes, las formas de razonar y proceder para comprender situaciones, solucionar problemas y hacer en contexto, lográndose con aquéllas notas establecer diferenciaciones significativas de los múltiples procesos y conductas pertenecientes alos campos cognoscitivo, afectivo y psicomotor de cada uno de los estudiantes.

  1. Formación de un panel de cata especializado en carne de vacuno : selección de catadores y entrenamiento del grupo

    OpenAIRE

    Oliván, M.C. (María); Guerrero, Luis

    2012-01-01

    Este trabajo trata del seguimiento para la formación de un panel de análisis sensorial aplicado a la carne de vacuno. Se basa en un conjunto de técnicas que permiten valorar las propiedades de los alimentos, es decir, los atributos de alos alimentos que se pueden detectar por los sentidos.

  2. ALOE-VERA GEL AS POTENTIAL CORROSION INHIBITOR FOR CONCRETE STEEL REINFORCEMENT

    Directory of Open Access Journals (Sweden)

    Héctor Herrera-Hernández

    2015-09-01

    Full Text Available In this work a GEL extracted from ALOE VERA leaves has been electrochemically studied as a possible corrosion inhibitor for concrete steel rebar. The corrosion resistance was evaluated by Electrochemical Impedance Spectroscopy (EIS measurements in hydrochloric acid solution (1M HCl as a function of GEL-inhibitor addition, uninhibited measurements were also discussed. The impedance results revealed that after addition of different GEL-extract proportion, the corrosion process of the steel exposed to acidic solution is remarkably inhibited, and it was also determined that the molecules of the GEL-extract follow a physisorption mechanism on the metal surface according to the Langmuir isotherm model with and adsorption standard free energy of about G°ads 14.17 kJ/mol. In this sense, the GEL extracted from ALOE-VERA leaves behaved as a mixed-type inhibitor.

  3. Preparation and characterization of chitosan/Aloe Vera composite nanofibers generated by electrostatic spinning

    Energy Technology Data Exchange (ETDEWEB)

    Ibrahim, Illani; Sekak, Khairunnadim Ahmad; Hasbullah, Norazurean [Center of Physics and Material Studies, Faculty of Applied Sciences, Universiti Teknologi Mara (UiTM) 40450 Shah Alam, Selangor (Malaysia)

    2015-08-28

    Researches on the fabrication of nanostructured based membrane have attracted great attention amongst scientists due to their wide potential applications on medical application. In this work, Chitosan and Aloe Vera sol-gel solution were electrospun using 20 kV DC supply at room temperature. Morphological structure and functional group of nanofibers were characterized using field emission scanning electron microscopy (FESEM) and Fourier-transform infrared spectroscopy (FT-IR) respectively. The optimum parameter obtained at 90% concentration of acetic acid, 0.3 ml/h of solution flow rate and 10 cm distance of nozzle to collector. The fiber diameters were analyzed using the ImageJ software. Average diameters of the Chitosan/Aloe Vera composite nanofibers is 183nm in ranges of 140–260nm.

  4. Role of Natural Immunomodulator (Aloe Vera in Cellular and Humoral Immune Responses

    Directory of Open Access Journals (Sweden)

    Ening Wiedosari

    2007-12-01

    Full Text Available Aloe vera belongs to a group of Liliaceae family plant and cultivated worldwide. It possesses acemannan (acetylated mannan, which has a significant pharmacological property. The acemannan has an immunomodulatory activity when administered to animals. The major immunomodulating effect includes the activation of immune effector cells, such as lymphocytes and macrophages, resulting in the production of cytokines, interleukin (IL-1, IL-6, IL-12 and tumor necrosis factor alpha (TNFα. In particular, this extract can modulate the differentiation capacity of CD4+T cells to mature into Th1 subsets and enhance the innate cytokine response. As a consequence, this extract will have a profound effect in controlling disease, caused by intracellular infectious agents (bacteria and viruses. However, further studies are needed to determine the immunomodulating effects of Aloe vera in multi-component extracts equivalent to what are being used commonly in traditional medicine.

  5. Impedance studies of a green blend polymer electrolyte based on PVA and Aloe-vera

    Science.gov (United States)

    Selvalakshmi, S.; Mathavan, T.; Vijaya, N.; Selvasekarapandian, Premalatha, M.; Monisha, S.

    2016-05-01

    The development of polymer electrolyte materials for energy generating and energy storage devices is a challenge today. A new type of blended green electrolyte based on Poly-vinyl alcohol (PVA) and Aloe-vera has been prepared by solution casting technique. The blending of polymers may lead to the increase in stability due to one polymer portraying itself as a mechanical stiffener and the other as a gelled matrix supported by the other. The prepared blend electrolytes were subjected to Ac impedance studies. It has been found out that the polymer film in which 1 gm of PVA was dissolved in 40 ml of Aloe-vera extract exhibits highest conductivity and its value is 3.08 × 10-4 S cm-1.

  6. Preparation and characterization of chitosan/Aloe Vera composite nanofibers generated by electrostatic spinning

    Science.gov (United States)

    Ibrahim, Illani; Sekak, Khairunnadim Ahmad; Hasbullah, Norazurean

    2015-08-01

    Researches on the fabrication of nanostructured based membrane have attracted great attention amongst scientists due to their wide potential applications on medical application. In this work, Chitosan and Aloe Vera sol-gel solution were electrospun using 20 kV DC supply at room temperature. Morphological structure and functional group of nanofibers were characterized using field emission scanning electron microscopy (FESEM) and Fourier-transform infrared spectroscopy (FT-IR) respectively. The optimum parameter obtained at 90% concentration of acetic acid, 0.3 ml/h of solution flow rate and 10 cm distance of nozzle to collector. The fiber diameters were analyzed using the ImageJ software. Average diameters of the Chitosan/Aloe Vera composite nanofibers is 183nm in ranges of 140-260nm.

  7. Aloe vera : Potential candidate in health management via modulation of biological activities

    Directory of Open Access Journals (Sweden)

    Arshad H Rahmani

    2015-01-01

    Full Text Available Treatment based on natural products is rapidly increasing worldwide due to the affordability and fewer side effects of such treatment. Various plants and the products derived from them are commonly used in primary health treatment, and they play a pivotal role in the treatment of diseases via modulation of biochemical and molecular pathways. Aloe vera, a succulent species, produces gel and latex, plays a therapeutic role in health management through antioxidant, antitumor, and anti-inflammatory activities, and also offers a suitable alternative approach for the treatment of various types of diseases. In this review, we summarize the possible mechanism of action and the therapeutic implications of Aloe vera in health maintenance based on its modulation of various biological activities.

  8. Nutritional content of fresh, bee-collected and stored pollen of Aloe greatheadii var. davyana (Asphodelaceae).

    Science.gov (United States)

    Human, Hannelie; Nicolson, Sue W

    2006-07-01

    Aloe greatheadii var. davyana is the most important indigenous South African bee plant. Fresh, bee-collected and stored pollen of this aloe was collected and analysed for its nutritional content, including amino acid and fatty acid composition. Highly significant differences were found between the three types of pollen. Collection and storage by the bees resulted in increased water (13-21% wet weight) and carbohydrate content (35-61% dry weight), with a resultant decrease in crude protein (51-28% dry weight) and lipid content (10-8% dry weight). Essential amino acids were present in equal or higher amounts than the required minimum levels for honeybee development, with the exception of tryptophan. Fatty acids comprised a higher proportion of total lipid in fresh pollen than in bee-collected and stored pollen. This study is the first to compare the changes that occur in pollen of a single species after collection by honeybees.

  9. Aloe L.--a second plant family without (TTTAGGG)n telomeres.

    Science.gov (United States)

    Adams, S P; Leitch, I J; Bennett, M D; Leitch, A R

    2000-06-01

    The physical ends of chromosomes are protected and stabilised by telomeres. The sequence of telomeric DNA normally consists of a simple repeating unit that is conserved in many organisms. Most plants examined have been shown to possess Arabidopsis-type telomeres consisting of many repeat copies of the sequence 5'-TTTAGGG-3'. Using fluorescent in situ hybridisation, slot blotting and the asymmetric polymerase chain reaction we demonstrate an absence of Arabidopsis-type telomeres in the genus Aloe (family Asphodelaceae). The only other plant genera so far reported without such telomeres are Allium, Nothoscordum, and Tulbaghia (family Alliaceae). As these genera and Aloe are petaloid monocots in the Asparagales, it is suggested that an absence of Arabidopsis-type telomeres may be characteristic of this related group of plants.

  10. Anti-tumor activity of Aloe vera against DMBA/croton oil-induced skin papillomagenesis in Swiss albino mice.

    Science.gov (United States)

    Saini, M; Goyal, Pradeep Kumar; Chaudhary, Geeta

    2010-01-01

    Human populations are increasingly exposed to various carcinogens such as chemicals, radiation, and viruses in the environment. Chemopreventive drugs of plant origin are a promising strategy for cancer control because they are generally nontoxic or less toxic than synthetic che-mopreventive agents, and can be effective at different stages of carcinogenesis. The present investigation was undertaken to explore the antitumor activity of topical treatment with aloe vera (Aloe vera) gel, oral treatment with aloe vera extract, and topical and oral treatment with both gel and extract in stage-2 skin carcinogenesis in Swiss albino mice induced by 7,12-dim ethylbenz(a)anthracene (DMBA) and promoted croton (Croton tiglium) oil. The animals were randomly divided into 4 groups and treated as follows: Group I, DMBA + croton oil only (controls); Group II, DMBA + croton oil + topical aloe vera gel; Group III, DMBA + croton oil + oral aloe vera extract; Group I V, DMBA + croton oil + topical aloe vera gel + oral aloe vera extract. Results showed that body weight was significantly increased from 78.6% in the control group (Group I) to 92.5%, 87.5%, and 90.0% in Groups II, III, and I V, respectively. A 100% incidence of tumor development was noted in Group I, which was decreased to 50%, 60%, and 40% in Groups II, III, and I V, respectively. Also in Groups II, III, and IV, the cumulative number of papillomas was reduced significantly from 36 to 12, 15, and 11; tumor yield from 3.6 to 1.2, 1.5, and 1.1; and tumor burden from 3.6 to 2.4, 2.50, and 2.75, respectively, after treatment with aloe vera. Conversely, the average latent period increased significantly from 4.9 (Group I) to 5.23, 5.0, and 6.01 weeks in Groups II, III, and I V, respectively. We conclude that aloe vera protects mice against DMBA/croton oil-induced skin papillomagenesis, likely due to the chemopreventive activity of high concentrations of antioxidants such as vitamins A, C, and E; glutathione peroxidase; several

  11. High-performance nanothermite composites based on aloe-vera-directed CuO nanorods.

    Science.gov (United States)

    Patel, Vinay Kumar; Bhattacharya, Shantanu

    2013-12-26

    In this work, we demonstrate the development of high-performance nanothermite composites derived from super-reactive CuO nanorods oxidizers fabricated by simple biogenic routes using Aloe vera plant extracts. Nanorods of various length scales have been realized via simple sonoemulsion and solid-state biosynthesis routes using Aloe vera gel as a green surfactant promoting the directional growth of CuO nanorods in both solid and emulsion phase. The biosynthesized CuO nanorods (oxidizers)/fuel (nanoaluminum) composites ignited vigorously with abundant gas generation, developing high heat of reaction of 1.66 kJ g(-1) and very high pressurization rate of around 1.09 MPa μs(-1) and peak pressure of 65.4 MPa when blasted inside a constant volume pressure cell with a charge density of 0.2 g cm(-3). The pressurization rates so obtained are four times higher with twice the peak pressure in comparison to such nanothermites formulated via other available state of the art wet-chemical techniques, which reflects the catalytic role of Aloe vera surface functional groups (A. vera-sfg) enhancing the reactivity of CuO oxidizers with excess gas release rate during exothermic reaction with nanoaluminum. Through this work, Aloe vera gel has for the first time been identified as a novel biotemplate for green synthesis of nanorod structures of metal oxides, and we have also studied the utility of A. vera-sfg in the creation of super-reactive CuO oxidizers producing excellent heat of reaction and dynamic pressure characteristics as demanded in propellants, explosives, and pyrotechnics.

  12. Hepatoprotective potential of Aloe barbadensis Mill. against carbon tetrachloride induced hepatotoxicity.

    Science.gov (United States)

    Chandan, B K; Saxena, A K; Shukla, Sangeeta; Sharma, Neelam; Gupta, D K; Suri, K A; Suri, Jyotsna; Bhadauria, M; Singh, B

    2007-05-22

    Aloe barbadensis Mill. Syn. Aloe vera Tourn. ex Linn.(Liliaceae) has been used in variety of diseases in traditional Indian system of medicine in India and its use for hepatic ailments is also documented. In the present study an attempt has been made to validate its hepatoprotective activity. The shade dried aerial parts of Aloe barbadensis were extracted with petroleum ether (AB-1), chloroform (AB-2) and methanol (AB-3). The plant marc was extracted with distilled water (AB-4). All the extracts were evaluated for hepatoprotective activity on limited test models as hexobarbitone sleep time, zoxazolamine paralysis time and marker biochemical parameters. AB-1 and AB-2 were observed to be devoid of any hepatoprotective activity. Out of two active extracts (AB-3 and AB-4), the most active AB-4 was studied in detail. AB-4 showed significant hepatoprotective activity against CCl4 induced hepatotoxicity as evident by restoration of serum transaminases, alkaline phosphatase, bilirubin and triglycerides. Hepatoprotective potential was confirmed by the restoration of lipid peroxidation, glutathione, glucose-6-phosphatase and microsomal aniline hydroxylase and amidopyrine N-demethylase towards near normal. Histopathology of the liver tissue further supports the biochemical findings confirming the hepatoprotective potential of AB-4. The present study shows that the aqueous extract of Aloe barbadensis is significantly capable of restoring integrity of hepatocytes indicated by improvement in physiological parameters, excretory capacity (BSP retention) of hepatocytes and also by stimulation of bile flow secretion. AB-4 did not show any sign of toxicity up to oral dose of 2 g/kg in mice.

  13. The use of ALOS data in studying environmental changes in Malaysia

    OpenAIRE

    Lim, Hwee San; Jafri, Mohd Zubir Mat; Abdullah, Khiruddin; Saleh, Nasirun Mohd.; Tahrin, Norhaslinda Mohamed

    2011-01-01

    Remote sensing data have been widely used in environmental studies like land cover change, flood observation, environmental pollution monitoring. This study is dealing with obtaining land cover, flood observation and environmental pollution using ALOS data. With the availability of remotely sensed and in situ data sets the derivable geophysical parameters are water depth, sea surface temperature (SST) and sediment (suspended matter) concentration. Understanding of the optical properties of wa...

  14. Radiometric, geometric, and image quality assessment of ALOS AVNIR-2 and PRISM sensors

    Science.gov (United States)

    Saunier, S.; Goryl, P.; Chander, G.; Santer, R.; Bouvet, M.; Collet, B.; Mambimba, A.; Kocaman, Aksakal S.

    2010-01-01

    The Advanced Land Observing Satellite (ALOS) was launched on January 24, 2006, by a Japan Aerospace Exploration Agency (JAXA) H-IIA launcher. It carries three remote-sensing sensors: 1) the Advanced Visible and Near-Infrared Radiometer type 2 (AVNIR-2); 2) the Panchromatic Remote-Sensing Instrument for Stereo Mapping (PRISM); and 3) the Phased-Array type L-band Synthetic Aperture Radar (PALSAR). Within the framework of ALOS Data European Node, as part of the European Space Agency (ESA), the European Space Research Institute worked alongside JAXA to provide contributions to the ALOS commissioning phase plan. This paper summarizes the strategy that was adopted by ESA to define and implement a data verification plan for missions operated by external agencies; these missions are classified by the ESA as third-party missions. The ESA was supported in the design and execution of this plan by GAEL Consultant. The verification of ALOS optical data from PRISM and AVNIR-2 sensors was initiated 4 months after satellite launch, and a team of principal investigators assembled to provide technical expertise. This paper includes a description of the verification plan and summarizes the methodologies that were used for radiometric, geometric, and image quality assessment. The successful completion of the commissioning phase has led to the sensors being declared fit for operations. The consolidated measurements indicate that the radiometric calibration of the AVNIR-2 sensor is stable and agrees with the Landsat-7 Enhanced Thematic Mapper Plus and the Envisat MEdium-Resolution Imaging Spectrometer calibration. The geometrical accuracy of PRISM and AVNIR-2 products improved significantly and remains under control. The PRISM modulation transfer function is monitored for improved characterization. ?? 2006 IEEE.

  15. Study of trace metals concentration and antimicrobial properties of tropical Aloe vera plant from southern India

    Directory of Open Access Journals (Sweden)

    V. Subramani

    2014-06-01

    Full Text Available This study was carried out with an objective to investigate the antibacterial and antifungal potentials and trace metals concentrations in Aloe vera (Linn plant leaves. Fresh leaves of Aloe vera were collected from Tiruchirappalli district of Tamil Nadu during the period of February - March 2014. The 100 g of shade dried A. vera leave power was used to collect the methanol extraction of the test plant by the soxhlet apparatus. The extracted solutions were dried by hot air oven at 60 °C for 48-72 h for further analysis. The antimicrobial activity of Aloe vera methanol extract was examined with six various pathogenic microorganisms such as gram positive, gram negative and fungal strains using the disk diffusion test. The two tested concentrations such as 0.60 and 1.20 mg/disc produce zone of inhibition on muller hinton agar (MHA and potato dextrose agar (PDA plates for bacteria and fungi, respectively. In this study, higher (1.20 mg concentration got greater sensitivity than lower (0.60 mg concentration against all strains. All the microbial strains depict higher sensitivity to the higher concentration (1.2 mg / disc for the test sample when compared to the positive control except bacterial strains such as Aeromonas liquefaciens MTCC 2645 (B1. The trace metal analyses of the plants were also carried out. The mean concentration of trace metals such as cadmium (Cd, chromium (Cr, copper (Cu, iron (Fe, nickel (Ni, lead (Pb and zinc (Zn were 0.04, BDL, 0.06, 0.08, BDL, 0.02 and 0.22 mg kg-1, respectively. Therefore, it is signified that Aloe vera plant extract is safe to be used as an antimicrobial agent. Hence, throughout impoundment is needed to verify the trace metal levels in plants.

  16. Formulation and evaluation of topical polyherbal antiacne gels containing Garcinia mangostana and Aloe vera

    OpenAIRE

    Gowda Bhaskar; Shariff Arshia; S.R.B Priyadarshini

    2009-01-01

    The objective of the study was to develop a topical poly herbal gel for the treatment of mild acne vulgaris. Aqueous extracts of Garcinia mangostana and Aloe vera were formulated in an aqueous based carbopol-934(1%w/w) gel system. Preformulation studies on solubility, partition co-efficient, MIC, MBC were determined along with compatibility studies using a validated HPLC method. Six formulations of the gel were prepared by varying the proportions of polymers and evaluated for their physicoche...

  17. Preparation of Polyamide Nanocapsules of Aloe vera L. Delivery with In Vivo Studies

    OpenAIRE

    Esmaeili, Akbar; Ebrahimzadeh, Maryam

    2014-01-01

    Aloe vera is the oldest medicinal plant ever known and the most applied medicinal plant worldwide. The purpose of this study was to prepare polyamide nanocapsules containing A. vera L. by an emulsion diffusion technique with in vivo studies. Diethyletriamine (DETA) was used as the encapsulating polymer with acetone ethyl acetate and dimethyl sulfoxide (DMSO) as the organic solvents and Tween and gelatin in water as the stabilizers. Sebacoyl chloride (SC) monomer, A. vera L. extract, and olive...

  18. Preparation and characterization of aloe vera blended collagen-chitosan composite scaffold for tissue engineering applications.

    Science.gov (United States)

    Jithendra, Panneerselvam; Rajam, Abraham Merlin; Kalaivani, Thambiran; Mandal, Asit Baran; Rose, Chellan

    2013-08-14

    Collagen-Chitosan (COL-CS) scaffolds supplemented with different concentrations (0.1-0.5%) of aloe vera (AV) were prepared and tested in vitro for their possible application in tissue engineering. After studying the microstructure and mechanical properties of all the composite preparations, a 0.2% AV blended COL-CS scaffold was chosen for further studies. Scaffolds were examined by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), and thermogravimetry analysis (TGA) to understand the intermolecular interactions and their influence on the thermal property of the complex composite. Swelling property in phosphate buffered saline (pH 7.4) and in vitro biodegradability by collagenase digestion method were monitored to assess the stability of the scaffold in a physiological medium in a hydrated condition, and to assay its resistance against enzymatic forces. The scanning electron microscope (SEM) image of the scaffold samples showed porous architecture with gradual change in their morphology and reduced tensile properties with increasing aloe vera concentration. The FTIR spectrum revealed the overlap of the AV absorption peak with the absorption peak of COL-CS. The inclusion of AV to COL-CS increased the thermal stability as well as hydrophilicity of the scaffolds. Cell culture studies on the scaffold showed enhanced growth and proliferation of fibroblasts (3T3L1) without exhibiting any toxicity. Also, normal cell morphology and proliferation were observed by fluorescence microscopy and SEM. The rate of cell growth in the presence/absence of aloe vera in the scaffolds was in the order: COL-CS-AV > COL-CS > TCP (tissue culture polystyrene plate). These results suggested that the aloe vera gel-blended COL-CS scaffolds could be a promising candidate for tissue engineering applications.

  19. Spectral reflectance of Kelantan Estuary with ALOS data to estimate transparency

    Science.gov (United States)

    Syahreza, S.; MatJafri, M. Z.; Lim, H. S.

    2012-09-01

    The Kelantan estuary, located in the northeastern part of Peninsular Malaysia, is characterized by high levels of suspended sediments. Kuala Besar is the estuary of the river directly opposite South China Sea. Spectral reflectance (Rr) and transparency measurements were carried out in the Kelantan estuary. The objective in this study is to establish empirical relationships between spectral remote sensing reflectance in ALOS satellite imagery and water column transparency, i.e. nephelometric turbidity unit (NTU) and Secchi disc depth (SDD) through these numerous in situ measurements. We detected that remote sensing reflectance are linear and power regression functions against NTU and SDD. The results of this sampling show that the wavelengths range from 500-620 nm is the most suitable band for measuring water column transparency. The calibrated reflectance of ALOS AVNIR-2 bands was also regressed against NTU and SDD field data to derive two empirical equations for water transparency estimation. These equations were calculated using ALOS images data on June 12, 2010. The result obtained indicated that reliable estimates of turbidity and transparency values for the Kelantan Estuary, Malaysia, could be retrieved using this method.

  20. Comparison of Plaque Inhibiting Efficacies of Aloe Vera and Propolis Tooth Gels: A Randomized PCR Study

    Science.gov (United States)

    Sunkara, Musalaiah Svv; Pantareddy, Indeevar; Sudhakar, Sankaran

    2015-01-01

    Backgound and Aim Allopathic medications used for periodontal disease are known to be associated with various side effects. Hence a search for naturotherapies are on the rise. Among the natural pharmacons available aloevera and propolis are considered to be effective and free from adverse effects. Taking this into account, the present study was done to compare the plaque inhibiting efficacies of Aloe vera and Propolis tooth gels in patients with chronic periodontitis. Materials and Methods Forty patients diagnosed with chronic periodontitis were randomly allocated to groups A and B containing 20 patients each. Patients in group A were advised to use Aloe vera tooth gel while those in group B were advised to use Propolis tooth gel. Clinical and microbiologic parameters using Polymerase chain reaction (PCR) were recorded at baseline and after 3 months. Results Student t-test was performed for all the obtained results. In the Aloe vera group, comparison of baseline PCR and after 3 month results showed reduction only in P. gingivalis (p=0.001), where as statistically significant reduction in all the three red complex microorganisms was seen in propolis group. All the clinical parameters (Plaque Index, Gingival Index, Bleeding on Probing, Probing pocket Depth, and Clinical Attachment Level) in both the groups showed statistically significant reductions after 3 months. Conclusion Propolis showed a statistically significant reduction in plaque, microbiologic and clinical parameters. However, clinical trials of longer durations with larger sample sizes are required to evaluate the efficacy. PMID:26501001

  1. Aloe vera phenomenon: a review of the properties and modern uses of the leaf parenchyma gel

    Energy Technology Data Exchange (ETDEWEB)

    Grindlay, D.; Reynolds, T.

    1986-06-01

    The mucilaginous gel from the parenchymatous cells in the leaf pulp of Aloe vera has been used since early times for a host of curative purposes. This gel should be distinguished clearly from the bitter yellow exudate originating from the bundle sheath cells, which is used for its purgative effects. Aloe vera gel has come to play a prominent role as a contemporary folk remedy, and numerous optimistic, and in some cases extravagant, claims have been made for its medicinal properties. Modern clinical use of the gel began in the 1930s, with reports of successful treatment of X-ray and radium burns, which led to further experimental studies using laboratory animals in the following decades. The reports of these experiments and the numerous favourable case histories did not give conclusive evidence, since although positive results were usually described, much of the work suffered from poor experimental design and insufficiently large test samples. In addition some conflicting or inconsistent results were obtained. With the recent resurgence of interest in Aloe vera gel, however, new experimental work has indicated the possibility of distinct physiological effects. Chemical analysis has shown the gel to contain various carbohydrate polymers, notably either glucomannans or pectic acid, along with a range of other organic and inorganic components. Although many physiological properties of the gel have been described, there is no certain correlation between these and the identified gel components. 154 references.

  2. Antioxidant responses and salt stress tolerance of Aloe vera irrigated by seawater with different salinity

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The variations of antioxidant enzyme activities including superoxide dismutase (SOD: EC 1.15.1.1), peroxidase (POD: EC 1.11.1.7) and catalase (CAT: EC 1.11.1.6), lipid peroxidation and major electrolytes in Aloe vera irrigated for three years with seawater having different salinity were studied. The results indicate that POD activity increased significantly at 10% seawater level, whereas decreased at higher seawater levels. The SOD activity decreased with increasing seawater concentration except for treatment with 100% seawater (denoted as T100% ) under long-term salt stress. Salinity decreased CATactivity,and increased lipid peroxidation and cell membrane injury. In addition, Ca2+ content was high in Aloe irrigated by seawater of low salinity level, but low in Aloe irrigated by seawater of high salinity level. An opposite trend was observed for the effect of seawater on Na + content of plants. K + and Mg2 +contents remain relatively stable under various seawater levels, which benefit plant growth.

  3. Quality Characteristics, Nutraceutical Profile, and Storage Stability of Aloe Gel-Papaya Functional Beverage Blend

    Directory of Open Access Journals (Sweden)

    Pushkala Ramachandran

    2014-01-01

    Full Text Available Aloe vera gel, well known for its nutraceutical potential, is being explored as a functional ingredient in a wide array of health foods and drinks. Processing of exotic fruits and herbal botanicals into functional beverage is an emerging sector in food industry. The present study was undertaken to develop a spiced functional RTS beverage blend using Aloe gel (AG and papaya. Aloe gel (30%, papaya pulp (15%, spice extract (5%, and citric acid (0.1% were mixed in given proportion to prepare the blend with TSS of 15 °Brix. The product was bottled, pasteurized, and stored at room temperature. The quality characteristics and storage stability of the spiced beverage blend (SAGPB were compared with spiced papaya RTS beverage (SPB. Periodic analysis was carried out up to five months for various physicochemical parameters, sugar profile, bioactive compounds, microbial quality, instrumental color, and sensory acceptability. The SAGPB exhibited superior quality characteristics compared to SPB both in fresh and in stored samples. The SPB was acceptable up to four months and SAGPB for five months. The results indicate that nutraceutical rich AG could be successfully utilized to develop functional fruit beverages with improved quality and shelf life.

  4. In vivo tracing of organophosphorus pesticides in cabbage (Brassica parachinensis) and aloe (Barbadensis).

    Science.gov (United States)

    Qiu, Junlang; Chen, Guosheng; Zhou, Hong; Xu, Jianqiao; Wang, Fuxin; Zhu, Fang; Ouyang, Gangfeng

    2016-04-15

    In vivo solid-phase microextraction (SPME) sampling method coupled with gas chromatography-mass spectrometry (GC-MS) analysis was employed to trace the uptake and elimination of organophosphorus pesticides (OPPs) in two kinds of edible plants, cabbage (Brassica parachinensis) and aloe (Barbadensis). The metabolism of fenthion in aloe was also investigated by the liquid chromatography tandem mass spectrometry analysis (LC-MS/MS) to understand the fate of OPPs in living plants better. Transpiration stream concentration factor (TSCF) and depuration rate constants of the OPPs in living plants were obtained therein. The health risk of the OPPs treated aloe was estimated by the maximum residue limit (MRL) approach, and it revealed that the OPPs were rather safe for their fast degradable property. However, peak concentration of fenthion-sulfoxide was found to exceed the MRL and was higher than that of the parent fenthion, which indicated the potential risk of pesticide metabolites. This study highlighted the application of in vivo SPME for contaminant tracing in different living edible plants. The in vivo tracing method is very convenient and can provide more data to evaluate the risk of different pesticides, which are very important for the safety of agriculture production.

  5. On-orbit performance of the Compact Infrared Camera (CIRC) onboard ALOS-2

    Science.gov (United States)

    Sakai, Michito; Katayama, Haruyoshi; Kato, Eri; Nakajima, Yasuhiro; Kimura, Toshiyoshi; Nakau, Koji

    2015-10-01

    Compact Infrared Camera (CIRC) is a technology demonstration instrument equipped with an uncooled infrared array detector (microbolometer) for space application. Microbolometers have an advantage of not requiring cooling system such as a mechanical cooler and are suitable for resource-limited sensor systems. Another characteristic of the CIRC is its use of an athermal optical system and a shutterless system. The CIRC is small in size (approximately 200 mm), is light weight (approximately 3 kg), and has low electrical power consumption (Satellite-2 (ALOS- 2). Since the initial functional verification phase (July 4-14, 2014), the CIRC has demonstrated functions according to its intended design. We also confirmed that the noise equivalent differential temperature of the CIRC observation data is less than 0.2 K, the temperature accuracy is within ±4 K, and the spatial resolution is less than 210 m in the calibration validation phase after the initial functional verification phase. The CIRC also detects wildfires in various areas and observes volcano activities and urban heat islands in the operational phase. The other CIRC will be launched in 2015 onboard the CALorimetric Electron Telescope (CALET) of the Japanese Experiment Module (JEM) of the International Space Station. Installation of the CIRCs on the ALOS-2 and on the JEM/CALET is expected to increase the observation frequency. In this study, we present the on-orbit performance including observational results of the CIRC onboard the ALOS-2 and the current status of the CIRC onboard the JEM/CALET.

  6. Gastroprotective and Anti-ulcer activity of Aloe vera juice, Papaya fruit juice and Aloe vera and Papaya fruit combined juice in Ethanol induced Ulcerated Rats

    Directory of Open Access Journals (Sweden)

    S. Gopinathan

    2013-12-01

    Full Text Available Peptic ulcer is the most prevalent gastrointestinal disease. Even though a wide range of drugs are available for the treatment of peptic ulcer, but many of these do not fulfill all the requirements and have side effects. These factors have attracted researchers to investigate the natural products which have more efficacy, less side effects and less expensive for the treatment of peptic ulcer disease. In the present study the anti ulcer activity of (1 Aloe vera juice, (2 papaya fruit juice (3 Aloe vera and papaya fruit combined juice were investigated in the ethanol induced ulcerated rats. The administration of plant juices decreased the offensive factors like ulcer index and acid secretion and also reduced the amount of protein and carbohydrates in the stomach fluid. Further, plant juices increased the defensive factors like activity of oxidative enzymes such as superoxide dismutase and reduced glutathione. Activities of alkaline phosphatase and lipid peroxide were higher in the diseased condition and same were reduced after the treatment with plant juices. Content of haemoglobin and RBC and WBC counts were brought back to normalcy after the treatment with plant juices. The efficacy of plant juices was comparable with the reference drug- Ranitidine. The results of the present study reveal that the plant juices are having efficiency in the gastroprotective activity. It is recommended that the above said plant juices can be further studied for their anti ulcer efficacy in human subjects.

  7. Use of dexpanthenol and aloe vera to influence the irradiation response of the oral mucous membrane (mouse); Beeinflussung der Strahlenreaktion der Mundschleimhaut (Maus) durch Dexpanthenol {+-} Aloe vera

    Energy Technology Data Exchange (ETDEWEB)

    Schlichting, S. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Dresden Univ. (Germany); Spekl, K.; Doerr, W. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Dresden Univ. (Germany)]|[Experimentelles Zentrum, Medizinische Fakultaet Carl Gustav Carus, Dresden Univ. (Germany)

    2004-07-01

    In summarising the outcome of the present study it can be said spraying the agent on the oral mucous membrane once a day had an effect on the incidence of mucous membrane ulceration in the case of both placebo and dexpanthenol treatment. However, there was no significant difference between placebo and dexpanthenol treatment, the only finding being a slight prolongation of latency time through aloe vera. These experimental findings give good reason to critically reconsider the clinical use of dexpanthenol as a supportive treatment for the prevention of radiogenic mucositis enoralis following irradiation of tumours in the head and neck region. However thorough oral lavage is an effective means of moderating the irradiation response of the oral mucous membrane. [German] Zusammenfassend ist festzustellen, dass in den vorliegenden Versuchen mit einmal taeglichem Aufspruehen des Praeparates auf die Mundschleimhaut sowohl die Placebo - wie auch die Dexpanthenol-Behandlung die Inzidenz von Schleimhautulzerationen modifiziert hat; zwischen Placebo- und Dexpanthenol-Behandlung ergab sich jedoch kein signifikanter Unterschied. Lediglich eine geringe Verlaengerung der Latenzzeit durch Aloe vera war zu beobachten. Auf der Basis dieser experimentellen Ergebnisse muss der klinische Einsatz von Dexpanthenol im Rahmen der Supportivtherapie zur Prophylaxe der radiogenen Mucositis enoralis bei der Bestrahlung von Kopf-Hals-Tumoren kritisch ueberdacht werden. Regelmaessige, intensive Mundspuelungen sind jedoch ein probates Mittel zur Verminderung der Strahlenreaktion der Mundschleimhaut. (orig.)

  8. Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages.

    Science.gov (United States)

    Budai, Marietta M; Varga, Aliz; Milesz, Sándor; Tőzsér, József; Benkő, Szilvia

    2013-12-01

    Aloe vera has been used in traditional herbal medicine as an immunomodulatory agent inducing anti-inflammatory effects. However, its role on the IL-1β inflammatory cytokine production has not been studied. IL-1β production is strictly regulated both at transcriptional and posttranslational levels through the activity of Nlrp3 inflammasome. In this study we aimed to determine the effect of Aloe vera on the molecular mechanisms of Nlrp3 inflammasome-mediated IL-1β production in LPS-activated human THP-1 cells and monocyte-derived macrophages. Our results show that Aloe vera significantly reduced IL-8, TNFα, IL-6 and IL-1β cytokine production in a dose dependent manner. The inhibitory effect was substantially more pronounced in the primary cells. We found that Aloe vera inhibited the expression of pro-IL-1β, Nlrp3, caspase-1 as well as that of the P2X7 receptor in the LPS-induced primary macrophages. Furthermore, LPS-induced activation of signaling pathways like NF-κB, p38, JNK and ERK were inhibited by Aloe vera in these cells. Altogether, we show for the first time that Aloe vera-mediated strong reduction of IL-1β appears to be the consequence of the reduced expression of both pro-IL-1β as well as Nlrp3 inflammasome components via suppressing specific signal transduction pathways. Furthermore, we show that the expression of the ATP sensor P2X7 receptor is also downregulated by Aloe vera that could also contribute to the attenuated IL-1β cytokine secretion. These results may provide a new therapeutic approach to regulate inflammasome-mediated responses.

  9. The Protective Effect of Aloe Vera on Histological Structure of Endocrine Portion of Pancreas Gland in the Diabetic Rat

    Directory of Open Access Journals (Sweden)

    N Erfani-Majd

    2016-01-01

    Full Text Available Introduction: Since aloe vera plant has many medical benefits, the present study aimed to investigate the protective effects of Aloe vera gel on the pancreatic islets and beta cells. Methods: This experimental study consisted of 50 mature male rats aged 2-3 months and weighed 200-250 g, who were randomly divided into five groups (n=10. Group I (control did not receive any treatments, and group II were diabetized via Streptozotocin (IP in 65 mg/kg, whose blood sample was taken after one week. Rats with blood glucose more than 250 mg/dL were considered as diabetic. Group III diabetic rats received the Aloe vera gel daily with dosage of 400 mg/kg, and group IV diabetic rats received insulin in 10 units/rat. Group V involved healthy rats which received only Aloe vera gel. After the last Aloe vera gel administration, blood glucose and body weight of all groups were measured on 15th and 30th days. Animals were euthanized with ether. Then tissues samples were collected from pancreas gland and fixed in 10% neutral buffered formalin solution. The 5-6 µ sections were made by paraffin embedding method and stained using haematoxylin-eosin (H&E and Aldehyde fuchsin stains. Ultimately, the histomorphometrical parameters were evaluated. Results: The mean number and size of pancreatic islets and beta cells of Langerhans islets decreased significantly in the diabetic group compared to the control group. The number of beta cells and diameter of langerhans islets increased significantly in the rats treated by Aloe vera gel in comparison to diabetic group at the end of 15th and 30th days. Conclusion: Applying Aloe vera gel seems to improve the renewal and restoration of langerhans islets and beta cells of pancreas gland in the diabetic rat.

  10. Microwave-assisted fibrous decoration of mPE surface utilizing Aloe vera extract for tissue engineering applications.

    Science.gov (United States)

    Balaji, Arunpandian; Jaganathan, Saravana Kumar; Supriyanto, Eko; Muhamad, Ida Idayu; Khudzari, Ahmad Zahran Md

    2015-01-01

    Developing multifaceted, biocompatible, artificial implants for tissue engineering is a growing field of research. In recent times, several works have been reported about the utilization of biomolecules in combination with synthetic materials to achieve this process. Accordingly, in this study, the ability of an extract obtained from Aloe vera, a commonly used medicinal plant in influencing the biocompatibility of artificial material, is scrutinized using metallocene polyethylene (mPE). The process of coating dense fibrous Aloe vera extract on the surface of mPE was carried out using microwaves. Then, several physicochemical and blood compatibility characterization experiments were performed to disclose the effects of corresponding surface modification. The Fourier transform infrared spectrum showed characteristic vibrations of several active constituents available in Aloe vera and exhibited peak shifts at far infrared regions due to aloe-based mineral deposition. Meanwhile, the contact angle analysis demonstrated a drastic increase in wettability of coated samples, which confirmed the presence of active components on glazed mPE surface. Moreover, the bio-mimic structure of Aloe vera fibers and the influence of microwaves in enhancing the coating characteristics were also meticulously displayed through scanning electron microscopy micrographs and Hirox 3D images. The existence of nanoscale roughness was interpreted through high-resolution profiles obtained from atomic force microscopy. And the extent of variations in irregularities was delineated by measuring average roughness. Aloe vera-induced enrichment in the hemocompatible properties of mPE was established by carrying out in vitro tests such as activated partial thromboplastin time, prothrombin time, platelet adhesion, and hemolysis assay. In conclusion, the Aloe vera-glazed mPE substrate was inferred to attain desirable properties required for multifaceted biomedical implants.

  11. Efecto in vitro de aceites esenciales de tres especies de Lippia sobre Moniliophthora roreri (Cif. y Par. Evans et al., agente causante de la moniliasis del cacao (Theobroma cacao L.

    Directory of Open Access Journals (Sweden)

    Betty Stefany Lozada

    2012-04-01

    Full Text Available Se evaluó el efecto antifúngico in vitro de cinco aceites esenciales (AEs (AE1, AE2, AE3, AE4 y AE5 extraídos de Lippia origanoides, L. citriodora y L. alba sobre aislados de monilia (Moniliophthora roreri obtenidos de frutos de cacao infectados provenientes de San Vicente de Chucurí, Santander, Colombia. Las plantas de Lippia fueron colectadas en cinco localidades colombianas. Los aislados de monilia (M1, M2, M3, M4 y M5 fueron caracterizados por su morfología, germinación y crecimiento en medios de cultivo. La actividad antifúngica de diferentes concentraciones de los AEs fue evaluada contra el aislado M2 y la cepa de M. roreri (ATCC 64239, para determinar su efecto sobre la germinación y la inhibición del crecimiento micelial. Los AEs estudiados inhibieron 100% de la germinación y del crecimiento micelial cuando fueron utilizados en concentraciones de 800 - 1000 µg/ml. Concentraciones de 200 µg/ml también mostraron efecto sobre los aislamientos fúngicos, siendo los AEs obtenidos de L. origanoides (AE2 y AE3 los más activos. Estos estaban compuestos principalmente por timol, p-cimeno, g-terpineno, acetato de timilo, carvacrol, b-mirceno, trans-b-cariofileno. Diferencias significativas (P < 0.05 sobre la susceptibilidad se observaron entre las dos cepas fúngicas estudiadas, siendo en general más susceptible el aislado M2 que la cepa ATCC. Los AEs de L. origanoides son candidatos para ser usados como posibles biofungicidas en el control de la moniliasis. Son necesarios estudios futuros orientados a determinar la actividad in vivo antifúngica de estos AEs y sus principales componentes.

  12. Rasgos del temperamento de los perros domésticos (Canis familiaris: evaluaciones conductuales

    Directory of Open Access Journals (Sweden)

    ADRIANA JAKOVCEVIC

    2009-01-01

    Full Text Available Los rasgos del temperamento se definen como tendencias conductuales estables entre situaciones similares y a lo largo del tiempo. En el presente trabajo se revisan las pruebas conductuales diseñadas para la evaluación de rasgos aislados del temperamento en los perros domésticos. Para cada dimensión se describen los estímulos empleados, las respuestas evaluadas y el correlato psiológico de las mismas. Los rasgos más estudiados fueron la temerosidad, la agresividad y la sociabilidad. Sin embargo, sólo la primera cuenta con correlatos psiológicos bien establecidos. Finalmente, las evaluaciones conductuales resultan de suma importancia para la selección de los perros para las distintas funciones que cumplen en la sociedad humana: detección de drogas, compañía, guardia, rescate de personas, etc.

  13. Determinación de los multímeros del factor von Willebrand en población mexicana

    OpenAIRE

    Edgar Hernández-Zamora; César Zavala-Hernández; Martha Eva Viveros-Sandoval; Ángeles Ochoa-Rico; Carlos Martínez-Murillo; Elba Reyes-Maldonado

    2014-01-01

    Antecedentes: la enfermedad de von Willebrand es un padecimiento hereditario en el que la estructura, función y concentración del factor de von Willebrand están alteradas y, en consecuencia, también la interacción plaqueta-factor de von Willebrand-endotelio. En México no hay registros epidemiológicos de la enfermedad, sólo se han efectuado algunos estudios aislados desde el punto de vista clínico y hematológico. Material y métodos: estudio retrospectivo efectuado en 155 mexicanos mestizos, 75...

  14. DEVOLOPMENT OF COMPOSITION AND TECHNOLOGY OF EYE MEDICINAL FILMS WITH ALOE EXTRACT

    Directory of Open Access Journals (Sweden)

    Y. V. Shikova

    2016-01-01

    Full Text Available Drugs which stimulate cornea regeneration are an obligatory component of the inflammatory diseases and injuries of eyes treatment. Nowadays the choice for these drugs is not diverse. A search for new prolonged medical drugs in dosage forms of eye films with Aloe extract with germicide and regenerative properties is prospective nowadays. A choice for additive substances for production of eye films with Aloe extract and working out of an optimal extraction technology was the purpose of the study. Materials and methods. The work involved: Aloe liquid extract, methylcellulose (MC, polyvin-ylpyrrolidone (PVP, carboxymethylcellulose (CMC, glycerol plasticiser, polyethylene-400. Humidity of ophthalmic drug films was determined with a gravimetric method; films dimensions: thickness, length and width in mm were measured with calipers; pH of the aqueous solution in films was determined potentiometrically. Results and discussion. A satisfactory appearance (uniformity, goodgap from the substrate, the absence of cracks and ruptures, elasticity and film thickness was the criterion for selection of film formers at the initial stage. Based on the requirements for ocular dosage forms, the films included sodium tetraborate buffer solution, which provided stability during preparation and use. Based on the study of literature data the selected samples were administered with 10% of aloe liquid extract. Prepared homogeneous transparent polymer solutions were cast on glass petri dish, pretreated with ethyl alcohol layer with a thickness of 5 mm. Drying of the film mass was done at room temperature to a residual humidity of 5%. As the result of the studies we have chosen films with film former of methylcellulose. Plates were strong, elastic, homogeneous, without gaps transparencies odorless, 0.035 mm thick. The obtained films were carved oval shape with a scalpel 4.0 mm in width and 9 mm diameter. Conclusions. 1. We have designed an optimal structure and technology of

  15. UJI AKTIVITAS KOMBINASI EKSTRAK AIR LIDAH BUAYA ( Aloe vera L. DAN AKAR MANIS (Glycyrrhiza glabra L. SEBAGAI PENYUBUR RAMBUT

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    Sulistiorini Indriaty

    2016-05-01

    Full Text Available Aloe vera (Aloe vera L. contains amino acids, vitamins, folic acid, and liquorice (Glycyrrhiza glabra L containing glisirizin, and flavonoids, which have properties as fertilizer hair. This study aims to obtain a combination of extracts with activity most optimal fertilizer hair, made 3 combination with a combination of 1 (the water extract of liquorice concentration of 2.5% and 7.5% aloe vera, a combination of 2 (the water extract of liquorice concentrations of 2, 5% and 2.5% aloe vera, and combinations of 3 (the water extract of liquorice concentration of 5% and 7.5% aloe vera. Testing the activity of hair growth is done by measuring the rate of hair growth, hair growth acceleration and weight of rabbit hair for 28 days. Rabbit hair growth results showed the combination 1 has an average length of the largest hair with hair growth rate value of 2.97 cm, accelerated hair growth amounted to 0.15 cm / day on day 7 and weighing 0.13 gram hair.

  16. Transforming growth factor-β (TGF-β) activation in cutaneous wounds after topical application of aloe vera gel.

    Science.gov (United States)

    Takzaree, Nasrin; Hadjiakhondi, Abbas; Hassanzadeh, Gholamreza; Rouini, Mohammad Reza; Manayi, Azadeh; Zolbin, Masoumeh Majidi

    2016-12-01

    Aloe vera is a medicinal plant used to treat various skin diseases. The effects of using aloe vera gel on the healing process were investigated by microscopic methods, cell counting, and TGF-β gene expression in the wound bed. Sixty Wistar rats weighing 200-250 g were placed under anesthesia in sterile conditions. A square 1.5 cm × 1.5 cm wound was made on the back of the neck. The rats were divided into control and 2 experimental groups. Additionally, the control and experimental groups were separated into 3 subgroups corresponding to 4, 7, and 14 days of study. In the first experimental group, aloe vera was used twice on the wound. The second experimental group received aloe vera overtreatment once on the wound. The positive control group received daily application of 1% phenytoein cream following surgical wound creation. The control group did not receive any treatment. This tissue was examined using histological staining (H&E) and Masson's Trichrome. Wound surface and wound healing were evaluated separately. TGF-β gene expression was analyzed by RT-PCR. Results showed that fibroblasts in both experimental groups were significantly increased, thereby acceleration wound healing. Application of aloe vera gel will increase TGF-β gene expression, ultimately accelerating the wound healing process.

  17. Semen characteristics and sperm morphological studies of the West African Dwarf Buck treated with Aloe vera gel extract

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    Oyeyemi Matthew Olugbenga

    2011-01-01

    Full Text Available Background: Aloe vera (Aloe barbadensis miller is an evergreen perennial plant widely used in modern herbal practice and is often available in proprietary herbal preparations.Objective: This study was designed to investigate the semen picture and spermatozoa morphology of West African Dwarf (WAD bucks treated with Aloe vera extract.Materials and Methods: Twelve sexually matured WAD bucks, weighing between 11 and 15 kg, were used for the study. The bucks were first used as control (pre-treatment and later as two groups of six animals each. The first six bucks received 10 mls of the 3% extract while the other six received 10 mls of the 4% of the extract for a 7 day period. Semen was collected from both the 3 and 4% extract treated bucks for the control (pre-treatment, on days eight (first week post-treatment and fifteen (second week post-treatment in each case using the electroejaculation method. The spermiogram of the bucks were investigated using standard procedures. Data obtained were analyzed using two way ANOVA and significance reported at p<0.05.Results: The continuous administration of Aloe vera extract significantly (p<0.05 reduced sperm concentration, motility and percentage livability and resulted in increased sperm abnormalities in the WAD buck.Conclusion: Aloe vera adversely affected the spermiogram of bucks. The plant can reduce fertility in male animals and is therefore not recommended for medicinal purpose in male animals especially those used for breeding.

  18. Precipitation of hydroxyapatite on electrospun polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds for bone tissue engineering.

    Science.gov (United States)

    Shanmugavel, Suganya; Reddy, Venugopal Jayarama; Ramakrishna, Seeram; Lakshmi, B S; Dev, Vr Giri

    2014-07-01

    Advances in electrospun nanofibres with bioactive materials have enhanced the scope of fabricating biomimetic scaffolds for tissue engineering. The present research focuses on fabrication of polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds by electrospinning followed by hydroxyapatite deposition by calcium-phosphate dipping method for bone tissue engineering. Morphology, composition, hydrophilicity and mechanical properties of polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds along with controls polycaprolactone and polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds were examined by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle and tensile tests, respectively. Adipose-derived stem cells cultured on polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds displayed highest cell proliferation, increased osteogenic markers expression (alkaline phosphatase and osteocalcin), osteogenic differentiation and increased mineralization in comparison with polycaprolactone control. The obtained results indicate that polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds have appropriate physico-chemical and biological properties to be used as biomimetic scaffolds for bone tissue regeneration.

  19. Análisis de la unión del rotavirus a los glóbulos rojos y el efecto que tiene dicha unión en la capacidad infecciosa en líneas tumorales.

    OpenAIRE

    Martín Bautista, Liliana

    2015-01-01

    Los virus oncolíticos (VO) son una alternativa prometedora para el tratamiento del cáncer. Estos virus se caracterizan por ser partículas no patógenas que infectan específicamente células cancerosas provocando su muerte, mientras que no afectan los tejidos sanos. El grupo de biología molecular de virus de la Universidad Nacional de Colombia ha realizado trabajos que evalúan la capacidad oncolitica del rotavirus. Inicialmente se seleccionaron 5 aislados de rotavirus, que fueron cultivados en...

  20. Cohesión social como programa arquitectónico: genealogías tentativas del edificio UNCTADIII/CCMGM. / Social cohesion as an architecture Layout: the genealogic background of the UNCTADIII building.

    Directory of Open Access Journals (Sweden)

    David Maulén de los Reyes

    2011-12-01

    Full Text Available El edificio del actual Centro Cultural Gabriela Mistral es resultado de una singular historia arquitectónica, pero no es un hecho aislado en el desarrollo arquitectónico del siglo XX en Chile. En este artículo el autor ofrece una genealogía tentativa para los ideales tras este proyecto./ The Centro Cultural Gabriela Mistral is a singularity in the Chilean architecture, but this is not an isolated project. The author offers a genealogy for the ideological background of this building.

  1. Valor del grosor de la capa de fibras nerviosas de la retina medido mediante tomografía de coherencia óptica como marcador de daño neuroaxonal en la esclerosis múltiple

    OpenAIRE

    López Lizcano, Ruth María

    2012-01-01

    Estudio del valor del grosor de la Capa de Fibras Nerviosas de la Retina (CFNR) a lo largo de un año como marcador de daño neuroaxonal en pacientes con distintos estadios de Esclerosis Múltiple (EM): pacientes con síndrome clínico aislado (CIS) que es el estadio más inicial de enfermedad desmielinizante, pacientes con esclerosis múltiple remitente-recurrente (EMRR) y pacientes con esclerosis múltiple primaria-progresiva (EMPP) frente a sujetos sanos. Estudio de las posibles correlaciones exis...

  2. Toxicologic Assessment of a Commercial Decolorized Whole Leaf Aloe Vera Juice, Lily of the Desert Filtered Whole Leaf Juice with Aloesorb

    Directory of Open Access Journals (Sweden)

    Inder Sehgal

    2013-01-01

    Full Text Available Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies—examined grossly and microscopically—that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks.

  3. Effects of Six Weeks Endurance Training and Aloe Vera Supplementation on COX-2 and VEGF Levels in Mice with Breast Cancer

    Science.gov (United States)

    Shirali, Saeed; Barari, Alireza; Hosseini, Seyed Ahmad; Khodadi, Elaheh

    2017-01-01

    The aim of this study was to determine effects of six weeks endurance training and Aloe Vera supplementation on COX-2 and VEGF levels in mice with breast cancer. For this purpose, 35 rats were randomly divided into 5 groups: control (healthy) and 4 cancer groups: control (cancer only), training, Aloe Vera and Aloe Vera + training. Breast cancer tumors were generated in mice by implantind. The training program comprised six weeks of swimming training accomplished in three sessions per week. Training time started with 10 minutes on the first day and increased to 60 minutes in the second week and the water flow rate was increased from 7 to 15 liters per minute at a constant rate. Aloe Vera extract at a dose of 300 mg/kg BW was administrated to rats by intraperitoneal injection. At the end of the study period, rats were anesthetized and blood samples were taken. Significant differences were concluded at pAloe Vera extract caused significant decrease in the COX-2 level in the cancer group. Also, in the training (swimming exercise) and Aloe Vera + training cancer groups, we observed significant decrease in the VEGF level as compared to controls. Our results suggest that Aloe Vera and training inhibit the COX pathway and cause decrease production of prostaglandin E2. Hence administration of Aloe Vera in combination with endurance training might synergistically improve the host milieu in mice bearing breast cancers.

  4. Toxicologic assessment of a commercial decolorized whole leaf aloe vera juice, lily of the desert filtered whole leaf juice with aloesorb.

    Science.gov (United States)

    Sehgal, Inder; Winters, Wallace D; Scott, Michael; David, Andrew; Gillis, Glenn; Stoufflet, Thaya; Nair, Anand; Kousoulas, Konstantine

    2013-01-01

    Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL) aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies-examined grossly and microscopically-that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks.

  5. Aloe vera extract as a promising treatment for the quality maintenance of minimally-processed table grapes

    Directory of Open Access Journals (Sweden)

    Giuseppina Rosaria Antonella Alberio

    2015-06-01

    Full Text Available The effect of an edible film obtained from a commercial Aloe vera extract, on the quality maintenance of minimally processed grapes belonging to three different cultivars (Sugar One, Victoria and Black Magic was evaluated by enzymatic (PPO, PME, β-GAL, physicochemical (pH, acidity, °Brix, and sensorial methods. All the analyzed parameters were measured in extracts obtained from minimally processed grapes packaged in ordinary atmosphere and stored at 4 °C for 15 days. Samples dipped into Aloe vera showed significant differences (p≤0.05 compared to untreated ones. The determination of such parameters and the evaluation of consumer acceptability were helpful to determine the effectiveness of the post-harvest treatment with Aloe vera for a storage period of 15 days.

  6. Formation Mechanism and Emission Spectrum of AlO Radicals in Reaction of Laser-ablated Al Atom and Oxygen

    Institute of Scientific and Technical Information of China (English)

    ZHANG Shu-dong; LI Hai-Yang

    2003-01-01

    The emission spectrum of AlO radicals was analyzed in 440-540 nm in the reaction of laser ablated Al beam and O2. The carrier of spectrum was assigned to Δν=0, ±1, ±2 vibrational sequences of B2Σ+-X2Σ+ transition of AlO radicals, the observed maximum vibrational quantum number was ν′=6. The rotational and vibrational temperatures of B state were estimated at 3000 and 7500 K by spectrally simulating the rovibronic population distribution. There is a strong evidence that the production of excited Al(2S) atoms is essential to the formation of excited AlO radicals.

  7. Enhanced biocompatibility of ZnS:Mn quantum dots encapsulated with Aloe vera extract for therapeutic applications

    Science.gov (United States)

    Anilkumar, M.; Bindu, K. R.; Sneha Saj, A.; Anila, E. I.

    2016-08-01

    Toxicity of nanoparticles remains to be a major issue in their application to the biomedical field. Aloe vera (AV) is one of the most widely exploited medicinal plants that have a multitude of amazing properties in the field of medicine. Methanol extract of Aloe vera can be used as a novel stabilising agent for quantum dots to reduce toxicity. We report the synthesis, structural characterization, antibacterial activity and cytotoxicity studies of ZnS:Mn quantum dots synthesized by the colloidal precipitation method, using methanol extract of Aloe vera (AVME) as the capping agent. The ZnS:Mn quantum dots capped with AVME exhibit superior performances in biocompatibility and antibacterial activity compared with ZnS:Mn quantum dots without encapsulation. Project supported by the Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India.

  8. Aloe Vera External Use and Clinical Use Research Progress%芦荟临床外用研究进展

    Institute of Scientific and Technical Information of China (English)

    刘学华; 赵红影

    2011-01-01

    To research Aloe vera external use function of the clinical study on the review.Aloe vera has medical and health care, hairdressing, eating, and other functions, known as the "drug" and "natural beautician" and "family doctor", "miracle" laudatory name.In recent years, with the number of natural medicine research, Aloe become increasingly common attention of world medicine, especially the multipurpose development and research articles, application scope, increasing economic value.%对芦荟外用功能的临床应用研究进展进行了综述.芦荟具有医疗、保健、美容、食用、现赏等多种功能,近年来芦荟外用品的开发研究,倍受青睐,应用范围日趋扩大,成为全世界关注的药食多用植物.

  9. Effect of a dentifrice containing aloe vera on plaque and gingivitis control: a double-blind clinical study in humans

    Directory of Open Access Journals (Sweden)

    Sílvia Morgana Araújo de Oliveira

    2008-08-01

    Full Text Available The effect of Aloe vera on the reduction of plaque and gingivitis was evaluated in a randomized, parallel and double-blind clinical trial. Subjects were randomly allocated to the test group (n=15 - dentifrice containing Aloe vera - or the control group (n=15 - fluoridated dentifrice. Plaque index (PI and gingival bleeding index (GBI were assessed at days 0 and 30. Subjects were asked to brush their teeth with the control or test dentifrice, three times a day, during a 30-day period. There was a significant reduction on plaque and gingivitis in both groups, but no statistically significant difference was observed among them (p>0.01. The dentifrice containing Aloe vera did not show any additional effect on plaque and gingivitis control compared to the fluoridated dentifrice.

  10. In vitro assessment of the prebiotic potential of Aloe vera mucilage and its impact on the human microbiota.

    Science.gov (United States)

    Gullón, Beatriz; Gullón, Patricia; Tavaria, Freni; Alonso, José Luis; Pintado, Manuela

    2015-02-01

    Aloe vera mucilage is reported to be rich in acemannan that is a polysaccharide with a backbone of β-(1→4)-D-mannose residues acetylated at the C-2 and C-3 positions and contains some side chains of galactose and arabinose attached to the C-6 carbon. The evaluation of the prebiotic potential of Aloe vera mucilage was carried out by in vitro fermentation using intestinal microbiota from six healthy donors as the inoculum. The prebiotic activity was assessed through the quantification of short chain fatty acids (SCFA) and the evaluation of dynamic bacterial population in mixed faecal cultures by fluorescence in situ hybridization (FISH). Our findings support the possible incorporation of the Aloe vera mucilage in the development of a variety of food products known as prebiotics aimed at improving gastrointestinal health.

  11. Identificacion de marcadores genéticos del agente causal del marchitamiento del clavel fusarium oxysporum f.sp. dianthi mediante amplificacion arbitraria de fragmentos polimórficos de adn

    Directory of Open Access Journals (Sweden)

    Arbeláez G.

    1998-12-01

    Full Text Available La técnica de Amplificación Arbitraria de Fragmentos Polimórficos de ADN (RAPD fue utilizada para identificar marcadores genéticos útiles para el desarrollo de un método diagnóstico para Fusarium oxysporum f.sp. dianthi, el agente etiológico de la enfermedad del marchitamiento del clavel. Con el fin de identificar fragmentos genéticos característicos de este patógeno, un total de 18 aislados diferentes, provenientes de diferentes lugares del mundo y 17 cepas de F. oxysporum de otras formas especiales fueron amplificadas utilizando 15 iniciadores diferentes. Aunque ninguno de los iniciadores empleados en este estudio amplificó una banda común a todas las formas especiales dianthi, el iniciador OPA 17 mostró un patrón de RAPD que permitió la identificación de cuatro grupos polimórficos dentro de este grupo taxonómico. Este mismo iniciador, permitió la discriminación entre aislados de Fusarium oxysporum f.sp. dianthi y cepas de F. oxysporum de otras formas especiales. No se observó una correlación directa entre el patrón de RAPD y las razas reportadas para F. oxysporum f.sp. dianthi, previamente determinadas mediante ensayos biológicos por otros grupos de investigadores. Los análisis de hibridación molecular con fragmentos escogidos de estos patrones de RAPD, permitieron el reconocimiento selectivo de los cuatro grupos descritos. Los fragmentos genómicos identificados, son candidatos para el desarrollo de un  sistema diagnóstico por PCR para este patógeno del clavel.

  12. The no-observed-adverse-effect level (NOAEL) of baby aloe powder (BAP) for nutraceutical application based upon toxicological evaluation.

    Science.gov (United States)

    Kwack, Seung Jun; Do, Seon-Gil; Kim, Young Woo; Kim, Yeon-Joo; Gwak, Hyo-Min; Park, Hyun Jong; Roh, Taehyun; Shin, Min Kyung; Lim, Seong Kwang; Kim, Hyung Sik; Lee, Byung-Mu

    2014-01-01

    Aloe has been used in versatile herbal medications and nutraceuticals throughout history. Aloe is widely considered to be generally safe for humans and used globally. The effectiveness and pharmacological properties of aloe are dependent upon when the plant is collected. However, little is known about the toxicology of whole-body aloe collected within less than 1 yr. Based upon widespread exposure to aloe, it is important to determine a daily intake level of this chemical to ensure its safety for humans. To determine the no-observed-adverse-effect level (NOAEL) of baby aloe powder (BAP) for clinical application, Sprague-Dawley (SD) rats were treated orally for 4 wk with 4 different concentrations: 0, 0.125, 0.5, and 2 g/kg body weight (bw). In this study, no significant or dose-dependent toxicological effects of BAP were observed in biochemical or hematological parameters, urinalysis, clinical signs, body weight, and food and water consumption. There were changes in some biomarkers in certain treated groups compared to controls; however, all values were within their reference ranges and not dose-dependent. Based on these results, the NOAEL of BAP was estimated to be greater than 2 g/kg bw in male and 2 g/kg bw in female SD rats. Collectively, these data suggest that BAP used in this study did not produce any marked subacute toxic effects up to a maximum concentration of 2 g/kg bw, and thus use in nutraceuticals and in pharmaceutical and cosmetic applications at a concentration of >2 g/kg is warranted.

  13. Antitumor properties and modulation of antioxidant enzymes' activity by Aloe vera leaf active principles isolated via supercritical carbon dioxide extraction.

    Science.gov (United States)

    El-Shemy, H A; Aboul-Soud, M A M; Nassr-Allah, A A; Aboul-Enein, K M; Kabash, A; Yagi, A

    2010-01-01

    The aim of this study was to evaluate the potential anticancer properties and modulatory effect of selected Aloe vera (A. vera) active principles on antioxidant enzyme activities. Thus, three anthraquinones (Namely: aloesin, aloe-emodin and barbaloin) were extracted from A. vera leaves by supercritical fluid extraction and subsequently purified by high performance liquid chromatography. Additionally, the N-terminal octapeptide derived from verectin, a biologically active 14 kDa glycoprotein present in A. vera, was also tested. In vivo, active principles exhibited significant prolongation of the life span of tumor-transplanted animals in the following order: barbaloin> octapeptide> aloesin > aloe-emodin. A. vera active principles exhibited significant inhibition on Ehrlich ascite carcinoma cell (EACC) number, when compared to positive control group, in the following order: barbaloin> aloe-emodin > octapeptide > aloesin. Moreover, in trypan blue cell viability assay, active principles showed a significant concentration-dependent cytotoxicity against acute myeloid leukemia (AML) and acute lymphocytes leukemia (ALL) cancerous cells. Furthermore, in MTT cell viability test, aloe-emodin was found to be active against two human colon cancer cell lines (i.e. DLD-1 and HT2), with IC(50) values of 8.94 and 10.78 microM, respectively. Treatments of human AML leukemic cells with active principles (100 microg ml(-1)) resulted in varying intensities of internucleosomal DNA fragmentation, hallmark of cells undergoing apoptosis, in the following order: aloe-emodin> aloesin> barbaloin> octapeptide. Intererstingly, treatment of EACC tumors with active principles resulted in a significant elevation activity of key antioxidant enzymes (SOD, GST, tGPx, and LDH). Our data suggest that the tested A. vera compounds may exert their chemo-preventive effect through modulating antioxidant and detoxification enzyme activity levels, as they are one of the indicators of tumorigenesis. These

  14. Generation of the 30 M-Mesh Global Digital Surface Model by Alos Prism

    Science.gov (United States)

    Tadono, T.; Nagai, H.; Ishida, H.; Oda, F.; Naito, S.; Minakawa, K.; Iwamoto, H.

    2016-06-01

    Topographical information is fundamental to many geo-spatial related information and applications on Earth. Remote sensing satellites have the advantage in such fields because they are capable of global observation and repeatedly. Several satellite-based digital elevation datasets were provided to examine global terrains with medium resolutions e.g. the Shuttle Radar Topography Mission (SRTM), the global digital elevation model by the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER GDEM). A new global digital surface model (DSM) dataset using the archived data of the Panchromatic Remote-sensing Instrument for Stereo Mapping (PRISM) onboard the Advanced Land Observing Satellite (ALOS, nicknamed "Daichi") has been completed on March 2016 by Japan Aerospace Exploration Agency (JAXA) collaborating with NTT DATA Corp. and Remote Sensing Technology Center, Japan. This project is called "ALOS World 3D" (AW3D), and its dataset consists of the global DSM dataset with 0.15 arcsec. pixel spacing (approx. 5 m mesh) and ortho-rectified PRISM image with 2.5 m resolution. JAXA is also processing the global DSM with 1 arcsec. spacing (approx. 30 m mesh) based on the AW3D DSM dataset, and partially releasing it free of charge, which calls "ALOS World 3D 30 m mesh" (AW3D30). The global AW3D30 dataset will be released on May 2016. This paper describes the processing status, a preliminary validation result of the AW3D30 DSM dataset, and its public release status. As a summary of the preliminary validation of AW3D30 DSM, 4.40 m (RMSE) of the height accuracy of the dataset was confirmed using 5,121 independent check points distributed in the world.

  15. Propriedades farmacológicas da Aloe vera (L. Burm. f.

    Directory of Open Access Journals (Sweden)

    V.S. Freitas

    2014-06-01

    Full Text Available A Aloe vera (L. Burm.f. tem sido utilizada há milhares de anos na medicina tradicional para o tratamento de diversos males. O intuito desse trabalho foi o levantamento bibliográfico de artigos que evidenciassem a atividade farmacológica da Aloe vera. A revisão contemplou livros e periódicos nacionais e internacionais indexados nas bases de dados MEDLINE, LILACS e SciElo, nos idiomas português, inglês e espanhol, utilizando as palavras-chave citadas. Após o levantamento bibliográfico, constatou-se que várias atividades biológicas são atribuídas a Aloe vera. Evidências sugerem eficácia no tratamento da psoríase, herpes genital, queimaduras e hiperglicemia. Além disto, também foram demonstradas atividades antineoplásica, antimicrobiana, anti-inflamatória e imunomodulatória por estudos in vitro e in vivo, entretanto, na cicatrização de feridas, os resultados foram conflitantes. No tratamento de dermatite por radiação e em queimaduras solares sua eficácia não foi comprovada e foram relatados casos de hepatite aguda devido ao consumo de preparações orais. Tendo em vista as várias atividades comprovadas e poucos relatos acerca de sua contra indicação, conclui-se que o uso desta espécie corrobora o vasto uso popular.

  16. Mineral content and biochemical variables of Aloe vera L. under salt stress.

    Science.gov (United States)

    Murillo-Amador, Bernardo; Córdoba-Matson, Miguel Víctor; Villegas-Espinoza, Jorge Arnoldo; Hernández-Montiel, Luis Guillermo; Troyo-Diéguez, Enrique; García-Hernández, José Luis

    2014-01-01

    Despite the proven economic importance of Aloe vera, studies of saline stress and its effects on the biochemistry and mineral content in tissues of this plant are scarce. The objective of this study was to grow Aloe under NaCl stress of 0, 30, 60, 90 and 120 mM and compare: (1) proline, total protein, and enzyme phosphoenolpyruvate carboxylase (PEP-case) in chlorenchyma and parenchyma tissues, and (2) ion content (Na, K, Ca, Mg, Cl, Fe, P. N, Zn, B, Mn, and Cu) in roots, stems, leaves and sprouts. Proline and PEP-case increased as salinity increased in both parenchyma and chlorenchyma, while total protein increased in parenchyma and decreased in chlorenchyma, although at similar salt concentrations total protein was always higher in chlorenchyma. As salinity increased Na and Cl ions increased in roots, stems, leaves, while K decreased only significantly in sprouts. Salinity increases typically caused mineral content in tissue to decrease, or not change significantly. In roots, as salinity increased Mg decreased, while all other minerals failed to show a specific trend. In stems, the mineral concentrations that changed were Fe and P which increased with salinity while Cu decreased. In leaves, Mg, Mn, N, and B decreased with salinity, while Cu increased. In sprouts, the minerals that decreased with increasing salinity were Mg, Mn, and Cu. Zinc did not exhibit a trend in any of the tissues. The increase in protein, proline and PEP-case activity, as well as the absorption and accumulation of cations under moderate NaCl stress caused osmotic adjustment which kept the plant healthy. These results suggest that Aloe may be a viable crop for soil irrigated with hard water or affected by salinity at least at concentrations used in the present study.

  17. Aloe vera inhibits proliferation of human breast and cervical cancer cells and acts synergistically with cisplatin.

    Science.gov (United States)

    Hussain, Arif; Sharma, Chhavi; Khan, Saniyah; Shah, Kruti; Haque, Shafiul

    2015-01-01

    Many of the anti-cancer agents currently used have an origin in natural sources including plants. Aloe vera is one such plant being studied extensively for its diverse health benefits, including cancer prevention. In this study, the cytotoxic potential of Aloe vera crude extract (ACE) alone or in combination with cisplatin in human breast (MCF-7) and cervical (HeLa) cancer cells was studied by cell viability assay, nuclear morphological examination and cell cycle analysis. Effects were correlated with modulation of expression of genes involved in cell cycle regulation, apoptosis and drug metabolism by RT-PCR. Exposure of cells to ACE resulted in considerable loss of cell viability in a dose- and time-dependent fashion, which was found to be mediated by through the apoptotic pathway as evidenced by changes in the nuclear morphology and the distribution of cells in the different phases of the cell cycle. Interestingly, ACE did not have any significant cytotoxicity towards normal cells, thus placing it in the category of safe chemopreventive agent. Further, the effects were correlated with the downregulation of cyclin D1, CYP 1A1, CYP 1A2 and increased expression of bax and p21 in MCF-7 and HeLa cells. In addition, low dose combination of ACE and cisplatin showed a combination index less than 1, indicating synergistic growth inhibition compared to the agents applied individually. In conclusion, these results signify that Aloe vera may be an effective anti-neoplastic agent to inhibit cancer cell growth and increase the therapeutic efficacy of conventional drugs like cispolatin. Thus promoting the development of plant-derived therapeutic agents appears warranted for novel cancer treatment strategies.

  18. Effectiveness of the aqueous extract of aloe in the treatment of oral alveolitis

    Directory of Open Access Journals (Sweden)

    Isidro de Jesús Nápoles González

    2015-07-01

    Full Text Available Background: alveolitis is a complication that may appear after the extraction of a tooth. It is considered a dental emergency due to the intense pain. Its prevalence is worrisome for the dental services for the complications and disability that it can provoke in patients.Objective: to evaluate the effectiveness of the aqueous extract of aloe with pharmaceutical use in the treatment of alveolitis.Method: an experimental study was carried out with 100 patients that visited the Provincial Teaching Dental Clinic, “La Vigía” Clinic, and “Julio Antonio Mella” Polycilinic of Camaguey in 2014, and that were diagnosed with alveolitis. The sample was made up of 50 patients in the control group treated with Alvogyl and 50 patients in the study group treated with aloe.Results: most of the patients belonged to the 26 to 45 age group. At 24 hours of treatment the results on pain relief were similar for both groups, with an 18 % for the study group and a 16 % for the control group. At 48 hours the best results were found in the control group, with 62 % of patients with no pain, and only a 30 % in the study group. At 72 hours this tendency was kept with 90 % in the control group and 72 % in the study group. In the study group an 84 % of the patients with wet alveolitis felt pain relief.Conclusions: the aqueous extract of aloe is a natural drug which is effective in the treatment of alveolitis, mainly in wet alveolitis and those affecting the maxillary bones. There were no adverse reactions with none of the used medications.

  19. Continental Scale Vegetation Structure Mapping Using Field Calibrated Landsat, ALOS Palsar And GLAS ICESat

    Science.gov (United States)

    Scarth, P.; Phinn, S. R.; Armston, J.; Lucas, R.

    2015-12-01

    Vertical plant profiles are important descriptors of canopy structure and are used to inform models of biomass, biodiversity and fire risk. In Australia, an approach has been developed to produce large area maps of vertical plant profiles by extrapolating waveform lidar estimates of vertical plant profiles from ICESat/GLAS using large area segmentation of ALOS PALSAR and Landsat satellite image products. The main assumption of this approach is that the vegetation height profiles are consistent across the segments defined from ALOS PALSAR and Landsat image products. More than 1500 field sites were used to develop an index of fractional cover using Landsat data. A time series of the green fraction was used to calculate the persistent green fraction continuously across the landscape. This was fused with ALOS PALSAR L-band Fine Beam Dual polarisation 25m data and used to segment the Australian landscapes. K-means clustering then grouped the segments with similar cover and backscatter into approximately 1000 clusters. Where GLAS-ICESat footprints intersected these clusters, canopy profiles were extracted and aggregated to produce a mean vertical vegetation profile for each cluster that was used to derive mean canopy and understorey height, depth and density. Due to the large number of returns, these retrievals are near continuous across the landscape, enabling them to be used for inventory and modelling applications. To validate this product, a radiative transfer model was adapted to map directional gap probability from airborne waveform lidar datasets to retrieve vertical plant profiles Comparison over several test sites show excellent agreement and work is underway to extend the analysis to improve national biomass mapping. The integration of the three datasets provide options for future operational monitoring of structure and AGB across large areas for quantifying carbon dynamics, structural change and biodiversity.

  20. Determination of rotational temperature of AlO from the $B^{2}\\sum^{+} -X^{2}\\sum^{+}$ system

    Indian Academy of Sciences (India)

    M M Chaudhari; C T Londhe; S H Behere

    2006-03-01

    AlO molecule was excited in a DC arc in air running between two aluminium electrodes. Rotational structure of the (0,0) band of the $B^{2}\\sum^{+} -x^{2}\\sum^{+}$ system of AlO molecule was photographed in the first order of a 10.6 m concave grating spectrograph. Intensity distribution amongst the well-resolved rotational lines of R1 and R2 branches was recorded and the average rotational temperature calculated from these has been determined as 2880 ± 100 K.

  1. The effect of aloe vera bioactive level as feed additive on the egg performances of laying hens

    OpenAIRE

    I.A.K Bintang; A.P Sinurat; T Purwadaria

    2005-01-01

    A study on the use of aloe vera bioactives as feed additive in layer ration was conducted. One hundred and twenty pullets strain Isa Brown were allocated into 5 treatments with 6 replicates and 4 birds/replicate. The treatments were: control,control+antibiotic (50 ppm zinc bacitracin), and control+ aloe vera at three levels (0.25; 0.50 and 1.00 g/kg). The treatments were conducted in a completely randomized design. Parameter measured were first initial body weight, age at 1st lay, feed intake...

  2. Effects of different levels of Aloe vera gel as an alternative to antibiotic on performance and ileum morphology in broilers

    OpenAIRE

    Ali Mahdavi; Ahmad Zare Shahneh; Abolfazl Zarei; Babak Darabighane

    2011-01-01

    The present study attempts to analyze the effects of different levels of Aloe vera gel as an alternative to antibiotic, on performance and ileum morphology in broilers. Three hundred one-day old Ross 308 male broilers were used on a completely randomized design in 5 groups with 4 replicates, each consisting of 15 broilers. The groups included the control group (basal diet) and three groups with basal diet mixed with different levels of Aloe vera gel (1.5%, 2% and 2.5%). Finally, there was a g...

  3. 芦荟罐头生产工艺%Main Process of Aloe Can

    Institute of Scientific and Technical Information of China (English)

    赵华

    2005-01-01

    芦荟是一种百合科草本植物,民间自古就有使用芦荟的记载。芦荟及其变种各类很多,目前在我国种植和加工较多的库拉索芦荟(Aloe Vera L.)。木立芦荟(A. arborescens Mill)和华芦荟(A.Varavia),还有少量的皂质芦荟和开谱敦芦荟等。

  4. Histological study of wound repair with topical aloe vera gel in rat

    Directory of Open Access Journals (Sweden)

    Nasrin Takzaree

    2015-12-01

    Full Text Available Background: One of the most important issues in medical sciences is wound healing and repair. Application of natural ingredients and herbs for treating ulcers has been in the history of human life. Nowadays, due to the lack of side effects of medicinal plants and a variety of effective compounds in plants, as well as numerous disadvantages of synthetic drugs there has been tendency to use medicinal plants in clinic. Aloe vera is an herbal drug used for treatment of dermal diseases. In this study we evaluated, effects of aloe vera on the wound healing through the microscopic techniques and cell counting. Methods: In this experimental study, sixty Wistar rats weighing 200-250 g were placed under general anesthesia and sterile conditions. Then a square shape wound with 1.5´1.5 mm dimension was made on the back of the neck. Rats were randomly divided to control and experimental group’s. Each group was divided to three subgroups with 4, 7, and 14 study days. In 1st experimental group aloe vera was used twice on the wound surface and in 2nd experimental group was used once daily and the positive control group were applied phenytoin cream 1% from the zero days of surgery. The control group did not get any treatment on the wound surface. For histological studies, during the fourth, seventh and fourteenth day’s rats were sacrificed and samples were taken from the wound area and adjacent skin. After histological staining with hematoxylin and eosin (H&E and Masson's trichrome stains, the cells were counted, wound surface and wound healing were investigated. Results: The macroscopic and microscopic evaluation showed that wound healing increased because the fibroblast numbers in two experimental groups improved compared with control group. The percentage of wound healing on different days in the experimental and control groups were significant. Data were analyzed by using one-way ANOVA test and P< 0.05 was significant. Conclusion: Present study showed

  5. The effectiveness of Aloe vera barbadensis bioactives on laying hens on commercial farmers

    OpenAIRE

    Tiurma Pasaribu; A.P Sinurat; T Purwadaria

    2006-01-01

    A field trial was conducted to study the effectiveness of dry gel Aloe vera (DG) as a feed additive for laying hens in commercial farms. The trial was consisted of two treatments, one was control, commonly used farmer ration containing antibiotic of zinc bacitracin at 0.5 g/kg and the second feed containing DG equal to 1.0 g/kg. Every treatment had two replicates with 504 Loghman laying hens. The hen day production (% HD), egg weight, feed consumption, feed conversion ratio (FCR), egg quality...

  6. Notes on the typification of some species of Aloe (Asphodelaceae/Aloaceae

    Directory of Open Access Journals (Sweden)

    H. F. Glen

    1995-12-01

    Full Text Available Lecto- or neotypes are designated for Aloe thompsoniae Groenew.. A. micracantha Haw .. A. schmidticma Regel (a synonym of/4,  cooperi Baker. A. longistyla Baker. A. aristata Haw., A. longiaristata Schult. & Schult. f„ (a synonym of the preceding name, A. humilis (L. Mill. var.  incurva Haw.. A. glauca Mill., A. niuricata Schult. (a synonym of the preceding name. A. saponaria (Aiton Haw. var.  latifolia Haw. (a new synonym of A. maculata All. and A. pluridens Haw. The confusing citation of the type of A. thomcroftii Pole Evans is clarified.

  7. Diversidad de las bacterias rizosféricas asociadas a plantas de Typha dominguesis en humedales del río Almendares

    Directory of Open Access Journals (Sweden)

    Irina Salgado-Bernal

    2012-01-01

    Full Text Available La gestión ambiental de los humedales es de interés actual, pero los estudios relacionados con los microor- ganismos asociados a las plantas de estos ecosistemas todavía son limitados. El objetivo del trabajo fue caracterizar la diversidad de grupos bacterianos presentes en la rizosfera de plantas hidrófitas de humedales naturales, en cuanto a su micromorfología y respuestas fisiológico - bioquímicas frente a compuestos químicos de interés ambiental. Se estudiaron 58 aislados bacterianos de la rizosfera de Typha dominguensis, en humedales del río Almendares, La Habana. Se carac- terizaron micromorfológica, tintorial y culturalmente y se analizó su interacción con diferentes fuentes de carbohidratos, nitrógeno, fósforo y metales pesados. Se detectó la representación de cinco grandes grupos bacterianos diferentes, debido a la diversidad de características micromorfológicas y tintoriales, con amplia variedad de características culturales. Los aislados presentaron comportamientos fisiológico -bioquímicos diversos. Fueron capaces de crecer con diferentes fuentes de carbono (glucosa, manosa, xilosa, lactosa y almidón; el 41 % las asimiló todas y el 76 % al menos tres. Se encontraron aislados que realizaron reducción del nitrato (47 % y amonificación (67 %; algunos (60 % con potencialidad para acu- mular fosfato y se observaron diferentes patrones de multirresistencia a los metales. Se estudiaron por primera vez en Cuba las bacterias rizosféricas asociadas a Typha dominguensis en humedales ribereños, como un elemento a valorar en las estrategias de conservación y como agentes con uso potencial en la biorremediación.

  8. METABOLITOS AISLADOS DE Zanthoxylum rhoifolium

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    Luis Enrique Cuca Suárez

    2008-04-01

    Full Text Available Una nueva cumarina llamada 3-metoxi-4-(3-metilbut-2-enil-2H-cromen-2-ona fue aislada de la madera de Z. rhoifolium, junto con los compuestos, conocidos dictamnina y N-metilflindersina. Sus estructuras fueron elucidadas por RMN, incluyendo técnicas bidimensionales y por comparación con datos reportados en la literatura.

  9. EVALUACIÓN DE MICROORGANISMOS AISLADOS DE GALLINAZA POR SU POTENCIAL PARA EL BIOCONTROL DE FUSARIUM (F. OXYSPORUM EN PLÁNTULAS DE UCHUVA (PHYSALIS PERUVIANA EVALUATION OF MICROORGANISMS INSOLATED FROM HEN MANURE FOR THEIR POTENCIAL AS BIOCONTROL AGENTS OF FUSARIUM (F. OXYSPORUM IN GOOSEBERRY (PHYSALIS PERUVIANA SEEDLINGS

    Directory of Open Access Journals (Sweden)

    Jorge Enrique Rodríguez Amézquita

    2010-12-01

    Full Text Available En Colombia, las pérdidas económicas ocasionadas por Fusarium oxysporum en el cultivo de uchuva son considerables. Se evaluaron hongos y bacterias aislados de 2 fuentes de gallinaza, su potencial como agentes de biocontrol de este patógeno. La evaluación se realizó en cajas de Petri con PDA para lo cual se colocó en el centro de las mismas, un disco de 5 mm de diámetro colonizado por el patógeno y a 3 cm del centro, sobre los ejes horizontal y vertical, cada uno de los aislamientos de la gallinaza. Los aislamientos que mostraron antagonismo fueron posteriormente evaluados in vitro por su capacidad de restringir el crecimiento y esporulación de F. oxysporum. Cada uno de los aislamientos que mostró el mayor potencial antagónico fue inoculado simultáneamente con el patógeno en plántulas de uchuva y evaluado por sus efectos en contra de la incidencia de la enfermedad y la muerte de las plántulas. Los resultados indicaron que de los 39 microorganismos aislados de la gallinaza pura, 6 mostraron antagonismo contra F. oxysporum y entre ellos los más efectivos para restringir in vitro su crecimiento y esporulación fueron los hongos H2 y H6 y las bacterias B17 y B19. Las bacterias B17 y B19 resultaron ser las más efectivas en reducir no sólo la incidencia sino también la muerte de plántulas ocasionada por el patógeno. Según los resultados de la identificación, los hongos H2 y H6 pertenecen a los géneros Geotrichum sp. y Trichoderma sp, respectivamente y las bacterias B17 y B19 al género Bacillus.In Colombia, economic losses due to attack of Fusarium oxysporum in the gooseberry plantation are considerable. Fungi and bacteria isolated from 2 hen manure sources were evaluated for their potential as biological control agents of this pathogen. The evaluation was conducted in Petri dishes containing PDA by placing a 5 mm diameter disk, colonized by this pathogen, in the center of the plates and at 3 cm from the center, over the

  10. Campylobacter jejuni y Campylobacter coli en tres grupos de gallinas de diferente origen geográfico del sur de Chile Occurrence of Campylobacter jejuni and Campylobacter coli in three groups of hens of different geographic origin in Southern Chile

    Directory of Open Access Journals (Sweden)

    H. FERNANDEZ

    2000-01-01

    Full Text Available Se estudiaron 300 muestras fecales de gallinas obtenidas en tres sitios geográficos del sur de Chile (comunas de Loncoche, Valdivia y Puerto Montt, para conocer la prevalencia de Campylobacter jejuni y de C. coli en estas aves consideradas como reservorio. La prevalencia de especies termotolerantes del género Campylobacter fue del 25,7%, siendo C. jejuni aislado con una frecuencia del 76,6% y la de C. coli del 23,4%. Fueron encontrados sólo 2 de los 4 biotipos de C. jejuni, siendo aislado el biotipo II con mayor frecuencia (68.8%. En C. coli fueron encontrados los 2 biotipos descritos para esta especie, siendo, también, el biotipo II el más frecuenteIn order to establish the prevalence of Campylobacter jejuni and C. coli, 300 fecal samples obtained from hens in three different geographical places (Loncoche, Valdivia and Puerto Montt cities from Southern Chile were studied. The prevalence of the thermotolerant species of Campylobacter was 25.7%, being Campylobacter jejuni isolated with a frequency of 76.6% and C. coli with 23.4%. Only two of the four biotypes of C. jejuni were found, being biotype II the most frequent one (68.8%. The two biotypes described for C. coli were isolated, and biotype II was also the most frequent (68.8%.

  11. Análisis Exploratorio del Perfil Demandado para Administración y Dirección de Empresas: Implicaciones para el Área Contable

    OpenAIRE

    José Luis Arquero Montaño; José Antonio Donoso Anes; Sergio Manuel Jiménez-Cardoso; José María González González

    2009-01-01

    La inserción de España en el Espacio Europeo de Educación Superior desembocará en una modificación de la estructura, duración y contenido de las titulaciones. Estas modificaciones no deben entenderse como un acontecimiento aislado, sino como una secuencia más en el debate sobre la calidad de los títulos universitarios. El objetivo del trabajo es identificar las necesidades de formación percibidas por los egresados de Administración y Dirección de Empresas de la Universidad de Sevilla, profund...

  12. AISLAMIENTO Y ELUCIDACIÓN ESTRUCTURAL DE ALGUNOS METABOLITOS MAYORITARIOS DEL EXTRACTO ETANÓLICO DE Solanum cornifolium. SECCIÓN GEMINATA

    Directory of Open Access Journals (Sweden)

    Luis Enrique Cuca

    2008-02-01

    Full Text Available Del extracto etanólico de la parte aérea de Solanum cornifolium, utilizando técnicas cromatográficas para la separación y purificaciónde cada uno de los compuestos, fueron aislados dos esteroles, B-sitosterol y Estigmasterol, un triterpeno pentacíclico,Lupeol, y un nuevo alcaloide esteroidal 2-(-metil-tetrahirofurano-Solanidina.Sus estructuras fueron elucidadas por RMN, incluyendo técnicas 2D y por comparación con datos de la literatura.

  13. Evaluación del potencial biofertilizante de bacterias diazótrofas aisladas de suelos con cultivo de cacao (Theobroma cacao L.)

    OpenAIRE

    Adriana Zulay Argüello-Navarro; Laura Yolima Moreno-Rozo

    2014-01-01

    La adaptabilidad del cacao (Theobroma cacao L.) en condiciones adversas permite sugerir que la microbiota asociada con la rizosfera desempeña una función importante para la nutrición y el desarrollo de la planta. En el trabajo se evaluó el potencial biofertilizante de bacterias fijadoras de nitrógeno (diazótrofas) aisladas de suelos cacaoteros por la técnica de diluciones seriadas. Las bacterias se aislaron en medios semiselectivos (NFb, JMV, LGI, JNFb). Los aislados puros se caracterizaron m...

  14. Phytochemical screening of the exudate of Aloe otallensis and its effect on Leishmania donovani

    Directory of Open Access Journals (Sweden)

    Zerihun Tesfaye Nigusse

    2016-06-01

    Full Text Available Objective: To evaluate the antileishmanial activity of methanolic extract of Aloe otallensis (A. otallensis on the promastigote stage of Leishmania donovani (L. donovani as compared to standard drugs and to screen its phytochemical constituents. Methods: Phytochemical screening was done by using the method mentioned by Evans and Trease on methanolic extract of the exudates of Aloe otallensis leaves. The extract was also evaluated for in vitro antileishmanial activity against L. donavani which is found from the Parasitology Unit of Black Lion Hospital. The result was compared to standard drugs of sodium stibogluconate, milfostin and paramomycin. Results: The extract has a good antileishmanial activity with an IC50 of 0.1230 μg/mL on L. donovani (AM 563. The experimental data showed that relatively it had better activity than paramomycin and milfostin but less activity than sodium stibogluconate. The data analyses were done by GraphPad Prism version 5 software after it was read by ELISA reader at the wave length of 650 nm. The phytochemical screening of the exudates of A. otallensis showed the presence of phenol, alkaloid and saponin. Conclusions: The methanol extract of the exudates of A. otallensis has a good antileishmaniasis activity and this may be attributed to phenol, alkaloid and saponin present in the plant. But it needs further analysis for the conformation of which constituent presents in high concentration to know which one has the strongest effect.

  15. Joint Use of ALOS PALSAR and Landsat TM Images for Urban Change Detection

    Science.gov (United States)

    Xu, Jinyan; Zhang, Lu; Liao, Mingsheng; Wang, He

    2013-01-01

    It is an important issue for urban planning to monitor the growth and change information of urban areas using remote sensed images. The joint use of Landsat TM data and ALOS PALSAR quad-polarization data for extracting change information of urban areas is investigated. The potential application and the performance of the two data sets are evaluated. The processes including the extraction of features, the dual-threshold EM change detection based on canonical correlation analysis (CCA) and the detection based on random forest (RF) classification were the major steps. The six bands of Landsat TM data without the thermal band were obtained. The four quad-polarimetric features as R-L circular polarization correlation coefficient, the linear polarization correlation coefficient, the total power (TP) and the cross-polarization isolation (XPI) were extracted from ALOS PALSAR data. And the corresponding differential images were got. The dual-threshold EM change detection and the RF classification were carried out based on these images. Accuracy assessment was done and the results were analyzed and verified.

  16. Quality and Acceptability of Meat Nuggets with Fresh Aloe vera Gel.

    Science.gov (United States)

    Rajkumar, V; Verma, Arun K; Patra, G; Pradhan, S; Biswas, S; Chauhan, P; Das, Arun K

    2016-05-01

    Aloe vera has been used worldwide for pharmaceutical, food, and cosmetic industries due to its wide biological activities. However, quality improvement of low fat meat products and their acceptability with added Aloe vera gel (AVG) is scanty. The aim of this study was to explore the feasibility of using fresh AVG on physicochemical, textural, sensory and nutritive qualities of goat meat nuggets. The products were prepared with 0%, 2.5%, and 5% fresh AVG replacing goat meat and were analyzed for proximate composition, physicochemical and textural properties, fatty acid profile and sensory parameters. Changes in lipid oxidation and microbial growth of nuggets were also evaluated over 9 days of refrigerated storage. The results showed that AVG significantly (p<0.05) decreased the pH value and protein content of meat emulsion and nuggets. Product yield was affected at 5% level of gel. Addition of AVG in the formulation significantly affected the values of texture profile analysis. The AVG reduced the lipid oxidation and microbial growth in nuggets during storage. Sensory panelists preferred nuggets with 2.5% AVG over nuggets with 5% AVG. Therefore, AVG up to 2.5% level could be used for quality improvement in goat meat nuggets without affecting its sensorial, textural and nutritive values.

  17. Antifungal activity evaluation of Aloe arborescens dry extract against trichosporon genus yeasts

    Directory of Open Access Journals (Sweden)

    João Ricardo Bueno de Morais Borba

    2014-10-01

    Full Text Available The objective of this study was to investigate the antifungal activity of Aloe arborescens dry extract against Trichosporon genus yeast species. Extraction was carried out by means of a longitudinal incision in fresh leaves, which were collected on a vat, and the total volume was frozen and subsequently lyophilized. Then, 40 mg of the dry extract was dissolved in DMSO by gentle inversion in order to obtain a solution whose concentration was 4000 µg mL-1. This solution became limpid and slightly yellowish because the pigment of the latex was attenuated. It was performed serial dilutions from 2,000 to 15.625 µg mL-1 with RPMI-1640 broth. There was already no pigment in the first dilution of 2000 μg mL-1. It was analyzed fifteen strains of Trichosporon spp., and Candida albicans ATCC 10231 was used as control strain. We carried out the reading of microplates in the ELISA reader device at a wavelength of 530 nm, after incubation for 24 and 48 hours, and it was determinated the Minimum Inhibitory Concentration (MIC. The MIC50 value obtained for all Trichosporon species and for C. albicans was 500 µg mL-1. As a result, we concluded that Aloe arborescens dry extract has antifungal activity against Trichosporon yeasts.

  18. Investigating the capabilities of new microwave ALOS-2/PALSAR-2 data for biomass estimation

    Science.gov (United States)

    Anh, L. V.; Paull, D. J.; Griffin, A. L.

    2016-10-01

    Most studies indicate that L-band synthetic aperture radar (SAR) has a great capacity to estimate biomass due to its ability to penetrate deeply through canopy layers. Many applications using L-band space-borne data have showcased their own significant contribution in biomass estimation but some limitations still exist. New data have been released recently that are designed to overcome limitations and drawbacks of previous sensor generations. The Japan Aerospace Exploration Agency (JAXA) launched the new sensor ALOS-2 to improve wide and high-resolution observation technologies in order to further meet social and environmental objectives. In the list of priority tasks addressed by JAXA there are experiments utilizing these new data for vegetation biomass distribution measurement. This study, therefore, focused on investigating the capabilities of these new microwave data in above ground biomass (AGB) estimation. The data mode used in this study was a full polarimetric ALOS-2/PALSAR-2 (L-band) scene. The experiment was conducted on a portion of a tropical forest in a Central Highland province in Vietnam.

  19. Polymer fraction of Aloe vera exhibits a protective activity on ethanol-induced gastric lesions.

    Science.gov (United States)

    Park, Chul-Hong; Nam, Dong-Yoon; Son, Hyeong-U; Lee, Si-Rim; Lee, Hyun-Jin; Heo, Jin-Chul; Cha, Tae-Yang; Baek, Jin-Hong; Lee, Sang-Han

    2011-04-01

    For centuries, Aloe has been used as a herbal plant remedy against skin disorders, diabetes, and for its cardiac stimulatory activity. Here, we examined the gastroprotective effects of an Aloe vera polymer fraction (Avpf; molecular weight cut-off ≥50 kDa; 150 mg/kg body weight, p.o.) on an ethanol-induced gastric lesion mouse model. Mice pre-treated with Avpf had significantly fewer gastric lesions than their respective controls. To further examine the potential mechanism underlying this effect, we used reverse transcription-polymerase chain reaction to examine nitric oxide synthase and matrix metalloproteinase (MMP)mRNA expression on tissues from gastric lesions. Our results revealed that the mRNA expressions of inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS) were each reduced by ~50% in Avpf-treated mice vs. the controls, whereas, the mRNA expression levels of endothelial nitric oxide synthase remained unchanged. MMP-9, an index for gastric lesions, also alleviated the ethanol-treated gastric ulceration during Avpf treatment. These findings collectively suggest that Avpf significantly protects the gastric mucosa against ethanol-induced gastric damage, at least in part, by decreasing mRNA expression levels of not only iNOS and nNOS, but also MMP-9.

  20. Integrating ALOS-2 and Sentinel-1 InSAR data for systematic volcano deformation monitoring

    Science.gov (United States)

    Hickey, James; Biggs, Juliet; Ebmeier, Susanna; Parker, Amy

    2016-04-01

    The recent launches of the Sentinel-1 and ALOS-2 satellites provide a wealth of new Interferometric Synthetic Aperture Radar (InSAR) data for Earth observation purposes. We exploit these data for volcano deformation monitoring with a particular focus on Latin America, which is nominated as a priority target area under the Committee on Earth Observation Satellites volcano pilot program. By conducting an integrated survey that employs both Sentinel-1 and ALOS-2 we are able to achieve previously unprecedented levels of spatial and temporal resolution and combat decorrelation arising from vegetation cover. Latin America is an ideal target region as it has a diversity of geographic volcano locations (e.g., dense rainforest to high-altitude deserts), as well as abundant and varied volcanic activity. The numerous local volcano observatories can also directly benefit from this additional InSAR data when integrating it alongside ground-based observations. To further facilitate this impact we are directing our data outputs to a global volcano deformation database in near-real-time to provide a first-order access point for observatory staff and research scientists in need of satellite-derived ground-deformation results. We will draw upon a selection of case studies within Latin America to demonstrate our approach and how it can enhance volcano monitoring and eruption forecasting efforts.

  1. Influence of Aloe vera on water absorption and enzymatic in vitro degradation of alginate hydrogel films.

    Science.gov (United States)

    Pereira, Rúben F; Carvalho, Anabela; Gil, M H; Mendes, Ausenda; Bártolo, Paulo J

    2013-10-15

    This study investigates the influence of Aloe vera on water absorption and the in vitro degradation rate of Aloe vera-Ca-alginate hydrogel films, for wound healing and drug delivery applications. The influence of A. vera content (5%, 15% and 25%, v/v) on water absorption was evaluated by the incubation of the films into a 0.1 M HCl solution (pH 1.0), acetate buffer (pH 5.5) and simulated body fluid solution (pH 7.4) during 24h. Results show that the water absorption is significantly higher for films containing high A. vera contents (15% and 25%), while no significant differences are observed between the alginate neat film and the film with 5% of A. vera. The in vitro enzymatic degradation tests indicate that an increase in the A. vera content significantly enhances the degradation rate of the films. Control films, incubated in a simulated body fluid solution without enzymes, are resistant to the hydrolytic degradation, exhibiting reduced weight loss and maintaining its structural integrity. Results also show that the water absorption and the in vitro degradation rate of the films can be tailored by changing the A. vera content.

  2. Hypoglycemic and Hypolipidemic Effects of Aloe vera Extract Preparations: A Review.

    Science.gov (United States)

    Pothuraju, Ramesh; Sharma, Raj Kumar; Onteru, Suneel Kumar; Singh, Satvinder; Hussain, Shaik Abdul

    2016-02-01

    Obesity is considered to be an epidemic disease, and it is associated with several metabolic disorders. Pharmacological treatments currently available are not effective for prolonged treatment duration. So, people are looking toward new therapeutic approach such as herbal ingredients. Since ancient periods, different herbs have been used for remedy purposes such as anti-obesity, antidiabetes, and antiinflammatory. Among the several herbal ingredients, Aloe vera (Aloe barbadensis Miller) is widely used to curb the metabolic complications. Till date, reports are not available for the side effects of A. vera. Several researchers are used to different solvents such as aqueous solution, alcohol, ethanol, and chloroform for the A. vera extract preparations and studied their hypoglycemic and hypolipidemic effects in animal and human studies. Furthermore, little information was recorded with the active compounds extracted from the A. vera and their anti-obesity and antidiabetic effects in clinical studies. In this review, we made an attempt to compile all the available literature by using different search engines (PubMed, Scopus, and Google Scholar) on the A. vera extract preparations and the possible mechanism of action involved in carbohydrate and lipid metabolism.

  3. The effectiveness of Aloe vera barbadensis bioactives on laying hens on commercial farmers

    Directory of Open Access Journals (Sweden)

    Tiurma Pasaribu

    2006-06-01

    Full Text Available A field trial was conducted to study the effectiveness of dry gel Aloe vera (DG as a feed additive for laying hens in commercial farms. The trial was consisted of two treatments, one was control, commonly used farmer ration containing antibiotic of zinc bacitracin at 0.5 g/kg and the second feed containing DG equal to 1.0 g/kg. Every treatment had two replicates with 504 Loghman laying hens. The hen day production (% HD, egg weight, feed consumption, feed conversion ratio (FCR, egg quality comprising yolk colour, albumin and yolk weights, egg shell eight and thikness, and mortality were observed for 24 weeks. The results showed that feed consumption, % HD, egg weight, FCR, yolk colour, albumin weight, yolk weight, egg shell weight, and egg shell thickness were not significantly different (P>0.05 between the control and DG treatment, except for the Haugh unit (HU. Thus, it can be concluded that Aloe vera bioactives has the same effectiveness as antibiotic as a feed additive at the level of commercial farms.

  4. Phytochemical screening of the exudate of Aloe otallensis and its effect on Leishmania donovani

    Institute of Scientific and Technical Information of China (English)

    Zerihun Tesfaye Nigusse; WoldeaAbebe Wondifraw; Sefinew MigbaruAbate

    2016-01-01

    Objective: To evaluate the antileishmanial activity of methanolic extract of Aloe otallensis (A. otallensis) on the promastigote stage of Leishmania donovani (L. donovani) as compared to standard drugs and to screen its phytochemical constituents. Methods: Phytochemical screening was done by using the method mentioned by Evans and Trease on methanolic extract of the exudates of Aloe otallensis leaves. The extract was also evaluated for in vitro antileishmanial activity against L. donavani which is found from the Parasitology Unit of Black Lion Hospital. The result was compared to standard drugs of sodium stibogluconate, milfostin and paramomycin. Results: The extract has a good antileishmanial activity with an IC50 of 0.1230 μg/mL on L. donovani (AM 563). The experimental data showed that relatively it had better activity than paramomycin and milfostin but less activity than sodium stibogluconate. The data analyses were done by GraphPad Prism version 5 software after it was read by ELISA reader at the wave length of 650 nm. The phytochemical screening of the exudates of A. otallensis showed the presence of phenol, alkaloid and saponin. Conclusions: The methanol extract of the exudates of A. otallensis has a good anti-leishmaniasis activity and this may be attributed to phenol, alkaloid and saponin present in the plant. But it needs further analysis for the conformation of which constituent presents in high concentration to know which one has the strongest effect.

  5. A Randomised, Cross-Over, Placebo-Controlled Study of Aloe vera in Patients with Irritable Bowel Syndrome: Effects on Patient Quality of Life.

    Science.gov (United States)

    Hutchings, H A; Wareham, K; Baxter, J N; Atherton, P; Kingham, J G C; Duane, P; Thomas, L; Thomas, M; Ch'ng, C L; Williams, J G

    2011-01-01

    Background. Irritable bowel syndrome (IBS) is a chronic, difficult to treat condition. The efficacy of Aloe vera in treating IBS symptoms is not yet proven. The purpose of this study was to determine if Aloe vera is effective in improving quality of life. Methods. A multicentre, randomised, double-blind, cross-over placebo controlled study design. Patients were randomised to Aloe vera, wash-out, placebo or placebo, washout, Aloe vera. Each preparation (60 mL) was taken orally twice a day. Patient quality of life was measured using the Gastrointestinal Symptoms Rating Score, Irritable Bowel Syndrome Quality of Life, EuroQol and the Short-Form-12 at baseline and treatment periods 1 and 2. Results. A total of 110 patients were randomised, but only 47 completed all questionnaires and both study arms. Statistical analysis showed no difference between the placebo and Aloe vera treatment in quality of life. Discussion. This study was unable to show that Aloe vera was superior to placebo in improving quality of life. Drop outs and other confounding factors may have impacted on the power of the study to detect a clinically important difference. Conclusion. This study failed to find Aloe vera superior to placebo in improving quality of life proven Irritable Bowel Syndrome patients.

  6. Na4Ca3(AlO2)10:Eu2+,Mn2+荧光粉的发光特性%Photoluminescence Characteristics of Na4 Ca3(AlO2)10:Eu2+ ,Mn2+

    Institute of Scientific and Technical Information of China (English)

    王雪; 田莲花

    2011-01-01

    A novel phosphor Na4Ca3(AlO2) 10:Eu2+ was prepared by solid state reaction method in a thermal-carbon reducing atmosphere. The photoluminescence(PL) properties were investigated in this paper. The excitation spectrum of Na4Ca3(AlO2) 10:Eu2+ showed two absorption bands centered at 262 nm and 320 nm respectively. The photoluminescence spectrum of Na4Ca3(AlO2) 10:Eu2+ exhibited a single emission peak centered at 441 nm, which could be attributed to 5d-4f transition of Eu + . The excitation spectrum of Na4Ca3(AlO2) 10:Eu2+ showed absorption peak at about 440 nm monitoring at 542 nm, which shows spectral overlap between emission spectrum of Na4Ca3(AlO2) 10:Eu2+. Co-doped Mn2+ with Eu2+, the photoluminescence spectra of Na4Ca3(AlO2) 10:Eu2+ were observed two peaks centered at 441 nm and 542 nm corresponding to the 5d-4f transition of Eu2+ and the d-d transition of Mn2+ , respectively. With increasing the concentration of Mn2+ ,the emission intensity of 5d-4f transition of Eu2+ at 441 nm was significantly decreased,whereas the d-d transition of Mn2+ at 542 nm was found to increase. The decay lifetime for Eu2+ was found to decrease with increasing Mn2+ dopant content, which was strong evidence for the energy transfer from Eu2+ to Mn2+ . We were also interested in investigating the energy transfer efficiency ηT of Eu2+→Mn2+ . With increasing Mn2+ dopant content, the energy transfer efficiency ηT was found to increase gradually. According to the Dexter's energy transfer formula of multipolar interaction, it was demonstrated that the energy transfer from Eu2+ to Mn2+ was due to the electric quadripole-quadripole interaction of the resonance transfer. According to the CIE chromaticity coordinates of Na4Ca3(AlO2) 10:Eu2+ , it was clearly observed that the CIE chro-maticity coordinates with the increase of Mn content shifted from blue region to white region.

  7. Streptococcus pneumoniae serotypes isolated from the middle ear of Mexican children diagnosed with acute otitis media Serotipificación de Streptococcus pneumoniae aislados de líquido de oído medio en niños mexicanos con diagnóstico de otitis media aguda

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    Demóstenes Gómez-Barreto

    2011-06-01

    Full Text Available OBJECTIVE: The aim of this study was to identify the etiology and the serotypes of S. pneumoniae (Sp in Mexican children with acute otitis media (AOM. MATERIALS AND METHODS: The study includessamples frompatientsdiagnosed with AOM at the Federico Gomez Children's Hospital of Mexico (2002-2003,with positive culture for Sp bacteriologically confirmed in middle ear fluid obtained by tympanocentesis. All Sp were serotyped. A total of 138 samples from 135 children with AOM were included. RESULTS: Sp was isolated in 72 samples from 70 children. Sixty (85.7% were previously healthy and 10 (14.3% were immunocompromised. The most common serotypes were 6B and 19F (16.67%, and 6 A, 14 and 23F (15.27%. CONCLUSION: The distribution of serotypes among the children with AOM in the study is similar to that reported in developing cities, and 63.9% of the isolated serotypes are found to be included in the 7-Valent Pneumococcal Conjugate Vaccine (PCV, 68.1% in the 10-Valent PCV and 83.3% in 13-Valent PCV.OBJETIVO: Conocer la etiología y serotipos de S. pneumoniae (Sp en niños mexicanos, con otitis media aguda (OMA. MATERIAL Y MÉTODOS: Se incluyeron las muestras de pacientes con OMA del Hospital Infantil de México Federico Gómez (2002-2003, con cultivo positivo para Sp, (bacteriológicamente confirmados en el líquido del oído medio obtenido por timpanocentesis. Todos los Sp. fueron serotipificados. Se incluyeron 138 muestras de 135 niños con OMA. RESULTADOS: Sp. se aisló en 72 muestras de 70 niños: 60 (85.7% eran previamente sanos y 10 (14.3% eran inmunocomprometidos. Los serotipos más frecuentes fueron 6B y 19F (16.67%, y 6 A, 14 y 23F (15.27%. CONCLUSIONES: La distribución de los serotipos en niños con otitis media aguda fue similar a la reportada en ciudades en desarrollo y se observó que 63.9% de los serotipos aislados están incluidos en la vacuna conjugada 7-valente, 68.1% en la 10-valente y 83.3% en la 13-valente.

  8. Evaluation of mutagenicity and anti-mutagenicity of aloe-emodin and aloe extract%芦荟大黄素及芦荟提取物的诱变性和抗诱变性

    Institute of Scientific and Technical Information of China (English)

    袁亚; 陈维; 张立实

    2012-01-01

    OBJECTIVE: To evaluate the mutagenicity and anti-mutagenicity of aloe-emodin and aloe extract by in vitro micronucleus test in L5178Y cells. METHODS: For 4 concentration of both aloe-emodin and aloe extract, mutagenicity and anti-mutagenicity groups were set up, as well as solvent control, positive control and anti-mutagenicity control. The L5178Y cells were treated respectively for 12 h, and in vitro micronucleus test was analyzed by routine method. RERULTS: Aloe-emodin showed mutagenic effect at 6.67 μg/ml. Micronucleus rate at this dose showed a significant difference compared with the control group (P < 0.05), but aloe extract showed non-mutagenic effect in this study. Within a certain dose range, both aloe-emodin(0.22 μg/ml) and aloe extract(20 μg/ml) could exert antagonistic effect on the increase of micronucleus rate induced by MMS, showing a significant difference compared with the control group(P< 0.01). CONCLUSION: Under our experimental conditions, aloe-emodin showed weak mutagenic effect, while aloe extract was not found to have mutagenic effect. Both of them displayed anti-mutagenic activity.%目的:用L5178Y小鼠淋巴瘤细胞体外微核试验评价芦荟大黄素和芦荟提取物的诱变和抗诱变作用,为其安全性评价提供依据.方法:设溶剂对照、阳性对照和抗诱变对照,芦荟大黄素和芦荟提取物诱变和抗诱变试验各设4个剂量组,处理L5178Y细胞12h后按常规方法进行体外微核试验分析.结果:较高浓度(6.67μg/ml)的芦荟大黄素可致微核细胞率增加,与对照组比较,差异有统计学意义(P<0.05);而芦荟提取物未见此效应.在一定剂量范围内,芦荟大黄素(0.22~6μg/ml)和芦荟提取物(20~180μg/ml)对甲磺酸甲酯(MMS)所致微核细胞率均有一定程度的拮抗作用,与对照组比较,差异有统计学意义(P<0.01).结论:芦荟大黄素具有一定的诱变作用,而在本实验剂量范围内的芦荟提取物未见遗传毒性.两种

  9. Comparative study on the content of polysaccharide in Aloe barbadensis Miller and Aloe ;vera L. Var. Chinensis (Haw.) Berger%库拉索芦荟与斑纹芦荟的多糖含量比较研究

    Institute of Scientific and Technical Information of China (English)

    唐建红; 刘川玉; 何洁

    2016-01-01

    Objective To determine the content of polysaccharide in Aloe barbadensis Miller and Aloe vera L. Var. Chinensis(Haw.)Berger. Methods Carubinose was used as reference substance, measured and compared polysaccharide content with the method of UV spectrophotometry at 610 nm absorbance of growth years for 1, 2, 3, 4 and 5 years of Aloe barbadensis Miller and Aloe vera L. Var. Chinensis(Haw.)Berger. Results The mannose and the absorbance was linear (r=0.998 5) in the range of 0.05 - 0.60 g/L, and the content of Aloe barbadensis Miller polysaccharide in the measured growth period was respectively 1.201, 1.485, 1.665, 2.355 and 5.040 mg/g. The content of polysaccharide in Aloe vera L. Var. Chinensis(Haw.)Berger was 0.780, 0.900, 1.035, 1.650 and 2.355 mg/g. Conclusions The content of polysaccharide in Aloe barbadensis Miller and Aloe vera L. Var. Chinensis(Haw.)Berger differed in growth years, which increased with aging and polysaccharide content in the same age of Aloe barbadensis Miller was significantly higher than that of the Aloe vera L. Var. Chinensis(Haw.)Berger.%目的:测定不同年限的库拉索芦荟与斑纹芦荟的多糖含量。方法以D-甘露糖为对照品,采用紫外分光光度法,测定生长年限为1、2、3、4及5年的库拉索芦荟和斑纹芦荟的多糖含量,并对其进行比较分析。结果甘露糖在0.05~0.60 g/L范围内与吸光度呈良好的线性关系(r=0.9985),测得生长年限为1、2、3、4及5年的库拉索芦荟多糖含量分别为1.201、1.485、1.665、2.355、5.040 mg/g;斑纹芦荟的多糖含量分别为0.780、0.900、1.035、1.650、2.355 mg/g。结论不同生长年限的库拉索芦荟和斑纹芦荟的多糖含量有差别,其含量随年限增长而增加,相同年限库拉索芦荟的多糖含量明显高于斑纹芦荟。

  10. In vivo diabetic wound healing effect and HPLC–DAD–ESI–MS/MS profiling of the methanol extracts of eight Aloe species

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    Abeer M. El Sayed

    2016-06-01

    Full Text Available Abstract Genus Aloe, Xanthorrhoeaceae, is well distributed all over Egypt, and many species have been used as medicinal plants; mainly reported to prevent cardiovascular diseases, cancer and diabetes. This study attempts to analyze the secondary metabolites in the methanol extract of the leaves of eight Aloe species; A. vera (L. Burm. f., A. arborescens Mill., A. eru A. Berger, A. grandidentata Salm-Dyck, A. perfoliata L., A. brevifolia Mill., A. saponaria Haw. and A. ferox Mill. growing in Egypt. For this aim HPLC–DAD–MS/MS in negative ion mode was used. Although belonging to the same genus, the composition of each species presented different particularities. Seventy one compounds were identified in the investigated Aloe species, of which cis-p-coumaric acid derivaties, 3,4-O-(E caffeoylferuloylquinic acid and caffeoyl quinic acid hexoside were the most common phenolic acids identified. Aloeresin E and isoaloeresin D, 2'-O-feruloylaloesin were the common anthraquinones identified. Lucenin II, vicenin II, and orientin were the common identified flavonoids in the investigated Aloe species. 6'-Malonylnataloin, aloe-emodin-8-O-glucoside, flavone-6,8-di-C-glucosides could be considered as chemotaxonomic markers for the investigated Aloe species. The eight Aloe species had significant anti-inflammatory activity, in addition to the significant acceleration of diabetic wound healing in rats following topical application of the methanol extracts of their leaves. This is the first simultaneous characterization and qualitative determination of multiple phenolic compounds in Aloe species from locally grown cultivars in Egypt using HPLC–DAD–MS/MS, which can be applied to standardize the quality of different Aloe species and the future design of nutraceuticals and cosmetic preparations.

  11. Resistencia a antibióticos de bacilos GRAM negativos aislados en unidades de cuidados intensivos: Análisis comparativo de dos periódos (1998-2001 Bacterial resistance to antibiotics in gram negative isolates from intensive care units: Comparative analysis between two periods (1998-2001

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    C. H. Rodriguez

    2003-01-01

    Full Text Available Se comparó la incidencia relativa de las diferentes especies de bacilos gram-negativos y la resistencia a varios antibacterianos, en dos muestras de aislamientos clínicos correspondientes a cinco meses del año 1998 y del mismo período del año 2001, con el objetivo de conocer la evolución de ambos, frecuencia de cada especie como agente etiológico, y resistencia a antimicrobianos. Para ello se analizaron en cada período 100 aislamientos de bacilos gram-negativos obtenidos de muestras clínicas de pacientes internados en salas de cuidados intensivos del Hospital de Clínicas José de San Martín. Se determinó la especie bacteriana y la concentración inhibitoria mínima de cada antibiótico. Acinetobacter spp. fue el microorganismo más aislado en ambos períodos. El porcentaje de aislamientos resistentes a imipenem fue del 60%, mientras que a ciprofloxacina y cefalosporinas de tercera generación fue superior al 80%. En Klebsiella pneumoniae el porcentaje de aislamientos resistentes a cefalosporinas de tercera generación disminuyó del 71.4 al 30% (pThe incidence and drug susceptibility of gram-negative isolates from clinical samples of patients from different intensive care units at the Hospital de Clinicas José de San Martín were analysed. Two hundred isolates during the same five months period, in two different years (1998 and 2001 were obtained and evaluated. Acinetobacter spp., was the most frequently isolated microorganism. Resistance to imipenem was observed in 60% of these isolations while resistance to 3rd generation cephalosporin and ciprofloxacin was observed in more than 80%. Klebsiella pneumoniae was not resistant to imipenem, the resistance to 3rd and 4rth generation cephalosporins decreased from 71.4 to 30% of isolates (p<0.05, while ciprofloxacin resistance increased from 5 to 20% (p<0.05. An increasing resistance to imipenem in Pseudomonas aeruginosa was noted, from 15.4 to 68% (p<0.05%; to ciprofloxacin, from 31.4 to

  12. [Preparative isolation and purification of cinnamoyl-C-glycoside chromone from aloe vera by high-speed countercurrent chromatography].

    Science.gov (United States)

    Pan, Xia; Cao, Xueli; Dong, Yinmao; Zhao, Hua

    2005-01-01

    Aloe chromone is a group of anti-inflammatory and anti-tyrosinase constituents found in aloe vera leaves. High-speed countercurrent chromatography (HSCCC) is reported for the preparative isolation and purification of a chromone from aloe vera. The crude extract was obtained by a series of pretreatment of aloe vera leaves and extracted from decolorizing active carbon with methanol. Then the extract was distributed between dichloromethane and water, and the organic part was then subjected to HSCCC for the isolation of chromone constituents. The chromone compounds with a high performance liquid chromatographic grade (>95%) was isolated through two step HSCCC separations by employing two solvent systems composed of chloroform-methanol-water and dichloromethane-methanol-water at volume ratios of 4/3/2 and 5/4/2, respectively. The chromone was finally identified as cinnamoyl-C-glycoside chromone by ultraviolet (UV), fast atom bombardment mass spectrometry (FAB-MS), nuclear magnetic resonance (1H NMR and 13C NMR).

  13. The Impact of Aloe vera and Calendula on Perineal Healing after Episiotomy in Primiparous Women: A Randomized Clinical Trial

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    Farideh Eghdampour

    2013-11-01

    Full Text Available Introduction: Episiotomy is used for enlarging the perineum. Aloe vera and Calendula have been used for treating different diseases from ancient times, limited researches have been done regarding the healing of these plants. Since the effect of their ointment on episiotomy healing has not been studied, this study is being done for determining the impact of Aloe vera and Calendula on episiotomy healing in primiparous women. Methods: This clinical trial involves 111 qualified primiparous women admitted in Lolagar hospital. They were randomly categorized into three groups of control (n=1 and experimental (n=2 groups. The women in experimental group used Aloe vera and Calendula Ointment every 8 hours and the control group used hospital routine on episiotomy for 5 days. The data were collected by demographic questionnaire and redness, edema, ecchymosis, discharge and approximation scale (REEDA which investigated the episiotomy healing before and five days after intervention in two groups. ANOVA, Tukey test, Kruskal-wallis, Chi-square were used for data analysis. Results: The three groups do not have statistically significant different regarding demographic and other intervening variables. Comparing the mean of REEDA in five days after delivery showed statistically significant difference between control and experimental groups.Conclusion: According to the results, using Aloe vera and Calendula ointment considerably increases the speed of episiotomy wound healing so it can be used for quickening the episiotomy healing.

  14. A randomized, double‑blind clinical study to assess the antiplaque and antigingivitis efficacy of Aloe vera mouth rinse

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    Bathini Chandrahas

    2012-01-01

    Full Text Available Aims: To evaluate the efficacy of Aloe Vera mouth rinse on experimental plaque accumulation and gingivitis. Materials and Methods: In this randomized, controlled, and double-blind study, a total of 148 systemically healthy subjects were screened in the age group of 18-25 years. Finally, 120 subjects were requested to abstain from oral hygiene (tooth brushing for 14 days and used a specially fabricated plaque guard. Following cessation of tooth brushing in the specified area, the subjects were randomly divided into Group A (test group who received 100% Aloe vera, Group B (negative control group who received placebo (distilled water, and Group C (positive control group who received 0.2% chlorhexidine. The rinse regimen began on the 15 th day and continued for 7 days. Plaque accumulation was assessed by Plaque Index (PI and gingivitis was assessed by Modified Gingival Index (MGI and Bleeding Index (BI at baseline (0, 7 th , 14 th , and 22 nd days. Results: There was statistically significant decrease in PI, MGI, and BI scores after the rinse regimen began in both Group A (test group and Group C (chlorhexidine compared with Group B. Mouth wash containing Aloe vera showed significant reduction of plaque and gingivitis but when compared with chlorhexidine the effect was less significant. Conclusion: Aloe vera mouthwash can be an effective antiplaque agent and with appropriate refinements in taste and shelf life can be an affordable herbal substitute for chlorhexidine.

  15. Mosquitocidal and water purification properties of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus leaf extracts.

    Science.gov (United States)

    Ethanolic extracts of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus were tested for toxicity to 3rd instar Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti. Median lethal concentrations (LC50) were, respectively, 0.44%, 0.51%, 0.59% and 0.68%. Cynodon dactylon...

  16. Clinical and radiographic evaluation of zinc oxide with aloe vera as an obturating material in pulpectomy: An in vivo study

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    Abhishek Khairwa

    2014-01-01

    Full Text Available Background: Pulp therapy for pulpally involved primary teeth continues to be a challenge to clinicians. One of the major areas of continued research is in the area of finding obturating materials to suit the specific properties of these teeth. Zinc oxide eugenol is used frequently in pulpectomy for the obturation of the primary teeth. Aims: To evaluate clinically and radiographically a mixture of zinc oxide eugenol and aloe vera as an obturating material. Materials and Methods: A total of 50 children, aged between 4 and 9 years, who were screened for unilateral or bilateral carious deciduous molars were studied. Out of these, 15 children were randomly selected for endodontic treatment. Obturation was done with a mixture of zinc oxide powder and aloe vera gel. Clinical and radiographic evaluation was done after 7 days, 1 month, 3 months, 6 months, and 9 months. The data were statistically analyzed. Results and Conclusion: Endodontic treatment using a mixture of zinc oxide powder and aloe vera gel in primary teeth has shown good clinical and radiographic success. A detailed observational study with longer follow-up will highlight the benefits of aloe vera in primary teeth as an obturating medium.

  17. "Escherichia coli" productores de BLEE aislados de urocultivo: implicaciones en el diagnóstico y tratamiento de la infección urinaria

    OpenAIRE

    Hernández Álvarez, Elena

    2009-01-01

    En el momento de plantearnos el trabajo, las infecciones por Klebsiella pneumoniae y Escherichia coli productores de Betalactamasas de Espectro Extendido (BLEE) eran un problema en el hospital. Algunos datos empezaban a sugerir que las infecciones por E.coli productor de BLEE tambien podian ser un problema fuera del hospital. E. coli es la principal causa de infeccion del tracto urinario (ITU) tanto en pacientes hospitalizados como en las infecciones de la comunidad, donde se aisla en un porc...

  18. Comparative evaluation of Green Tea- Aloe Vera mouthwash and chlorhexidine 0.2% on gingival indices (A randomized clinical trial

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    Naser Sargolzaie

    2015-12-01

    Full Text Available Introduction: Nowadays in the field of dentistry, there is a trend to encourage the use of herbal and natural products. The high level of clinical research in terms of considering green tea and aloe vera, with various functionality for individual use is a typical example. The purpose of this randomized, controlled, and double-blind study was to evaluate comparative evaluation of green tea-aloe vera mouthwash and chlorhexidine 0.2% on gingival indices. Methods: A total of 60 patients (26 women and 34 men with periodontal disease were randomly allocated into one of the three double blind groups, 20 in each, to receive the following treatments :(1 0.2% chlorhexidine, (2 green tea-aloe vera and  (3 distilled water. Plaque and gingival indices were evaluated on the day of the beginning of the experiment and 14 days postoperative. Subjects were asked to rinse their mouth with the mouth rinse, twice a day, during a 14-day period. Paired t-test was used to test the mean difference on 0 and 14 days, respectively. One way analysis of variance (ANOVA was used to compare the mean difference between the groups while Tukey test was used for multiple comparisons. Results: Chlorhexidine 0.2%, green tea - aloe vera and placebo reduced the plaque index by 0.17±0.14, 0.10±0/08 and 0.02±0.18, respectively, with a statistical significance of p=0.008. This difference was related to Chlorhexidine 0.2% and green tea - aloe vera with Placebo. There exist a significant difference of p=0.001 in the bleeding index  between the three groups, and this difference was related to Chlorhexidine 0.2% and green tea - aloe vera with placebo. Conclusion: Green tea –aloe vera   mouthwash improves periodontal health status. Therefore, it can be used to improve oral and dental health status.

  19. Effects of the application of Aloe vera (L. and microcurrent on the healing of wounds surgically induced in Wistar rats Efeitos da aplicação de Aloe vera (L. e microcorrente no reparo de lesões cirúrgicas induzidas em ratos Wistar

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    Cristina Cruz Franchini

    2009-04-01

    Full Text Available PURPOSE: To investigate the effects of topical application of an Aloe vera gel combined or not with microcurrent application on the healing of skin wounds surgically induced in Wistar rats. METHODS: The animals were randomly divided into the following groups: control group, animals topically treated with Aloe vera, animals treated with a microcurrent, and animals receiving topical application of Aloe vera combined with microcurrent application. RESULTS: The results indicated differences in wound healing between the various treatments when compared to the control group. Tissue hyperplasia was lower in the control group compared to the other treated groups. Accelerated wound healing was observed in the group treated with Aloe vera compared to control. Animals submitted to microcurrent application only and the group treated with microcurrent plus Aloe vera presented an earlier onset of the proliferative phase compared to the control group and animals treated with Aloe vera gel alone. Morphometric data confirmed the structural findings. CONCLUSION: Simultaneous application of Aloe vera gel and microcurrent is an excellent choice for the treatment of open wounds thus indicating a synergistic action of these two applications.OBJETIVO: Investigar os efeitos da aplicação tópica do gel de Aloe vera, combinada ou não com a aplicação de microcorrente no reparo de lesões cutâneas induzidas cirurgicamente em ratos Wistar. MÉTODOS: Os animais foram distribuídos aleatoriamente em: grupo controle, tratado topicamente com gel in natura de Aloe vera, tratado com microcorrente e tratado com aplicação tópica de Aloe vera associada à microcorrente. RESULTADOS: Os resultados do presente trabalho indicaram que o reparo tecidual ocorreu de forma diferenciada nos vários tratamentos empregados quando comparados ao grupo controle. A hiperplasia tecidual no grupo controle foi menor que a observada nos demais grupos tratados. No grupo tratado com aplica

  20. Estudo da viabilidade econômica do cultivo da babosa (aloe vera L. Study of economic viability of the Aloe vera L. culture

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    Dionizio Bernardino Bach

    2007-08-01

    Full Text Available Conduziu-se este este trabalho, com o objetivo de estudar a viabilidade econômica da babosa e verificar se o cultivo desta planta é ou não, uma alternativa economicamente viável ao produtor rural. Os dados relativos ao custo de produção, utilizados neste trabalho, foram obtidos junto às áreas de produção e instalações industriais da NATURAMA - Indústria e Comércio de Produtos Agropecuários Ltda, estabelecida no município de Paulo Lopes, SC. Na determinação do custo de produção de um hectare de babosa, em cultivo orgânico, foram consideradas todas as etapas, desde a obtenção das mudas até a conclusão do processamento, quando o produto final encontra-se apto ao consumo, e neste caso, é uma bebida em forma de suco. O processamento dos dados foi realizado utilizando uma planilha eletrônica desenvolvida especificamente para esse fim, a qual estimou os custos utilizando as metodologias do custo total (custos fixos e variáveis e do custo operacional total (custo operacional efetivo e depreciações. Pela análise da rentabilidade, conclui-se que a atividade é economicamente viável.The aim of this work is to study the economic viability of Aloe vera L. and verify if the culture of this plant is or not an alternative economically viable for the farmer. The data of production cost used in this work were gotten in the areas of production and industrial installations of NATURAMA- Industry and Trade of Farming Products LTDA, settled in Paulo Lopes, SC. In the determination of the production cost of one Aloe vera L hectare, all the phases were taken into consideration, from the getting of molts up to the conclusion of the processing, when the final product is ready to the consumption, and in this case, it's a beverage in a way of juice. The processing data was made by using an electronic spread sheet developed specifically for this reason, which estimated the costs using the methodologies of the total cost (steady and variable

  1. Actividad molusquicida del Paraiso (Melia azedarach L. (Meliaceae sobre Lymnaea cubensis, molusco vector de Fasciolosis

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    Perez Marlene Piña

    1998-01-01

    Full Text Available INTRODUCCIÓN: La Fasciolosis constituye en diversas regiones una fuente importante de pérdidas económicas y cuando se descuidan las medidas de control de sus hospedadores (moluscos, junto a condiciones ecológicas favorables, pueden ocurrir casos aislados de Fasciolosis humana. Dentro de los métodos alternativos para su control está el uso de extractos vegetales y se pretendió evaluar el probable empleo del jugo extraído del fruto y semillas del Paraiso (Melia azedarach L. en el control de Lymnaea cubensis, principal vector de la Fasciolosis en Cuba. MATERIAL Y MÉTODO: Diferentes concentraciones del jugo extraído del fruto y semillas del Paraiso (Melia azedarach L. fueron testados para determinar las dosis letales media (DL50 y máxima (DL90 usando un programa computarizado Probit-Log. Siete series experimentales fueron probadas usando 72 moluscos en cada una. Para determinar la influencia sobre la frecuencia cardíaca fueron testados tres grupos de 10 moluscos, dos fueron tratados con las DL50=0,88627 y DL90=1,7641, respectivamente, mientras que el tercero fue considerado como testigo. RESULTADOS Y CONCLUSIONES: Se observó una marcada influencia de ambas dosis sobre la frecuencia cardíaca del molusco estudiado. Estos resultados son alentadores, pues demuestran el potencial empleo de esta planta en el control de moluscos indeseables.

  2. Actividad molusquicida del Paraiso (Melia azedarach L. (Meliaceae sobre Lymnaea cubensis, molusco vector de Fasciolosis

    Directory of Open Access Journals (Sweden)

    Marlene Piña Perez

    1998-06-01

    Full Text Available INTRODUCCIÓN: La Fasciolosis constituye en diversas regiones una fuente importante de pérdidas económicas y cuando se descuidan las medidas de control de sus hospedadores (moluscos, junto a condiciones ecológicas favorables, pueden ocurrir casos aislados de Fasciolosis humana. Dentro de los métodos alternativos para su control está el uso de extractos vegetales y se pretendió evaluar el probable empleo del jugo extraído del fruto y semillas del Paraiso (Melia azedarach L. en el control de Lymnaea cubensis, principal vector de la Fasciolosis en Cuba. MATERIAL Y MÉTODO: Diferentes concentraciones del jugo extraído del fruto y semillas del Paraiso (Melia azedarach L. fueron testados para determinar las dosis letales media (DL50 y máxima (DL90 usando un programa computarizado Probit-Log. Siete series experimentales fueron probadas usando 72 moluscos en cada una. Para determinar la influencia sobre la frecuencia cardíaca fueron testados tres grupos de 10 moluscos, dos fueron tratados con las DL50=0,88627 y DL90=1,7641, respectivamente, mientras que el tercero fue considerado como testigo. RESULTADOS Y CONCLUSIONES: Se observó una marcada influencia de ambas dosis sobre la frecuencia cardíaca del molusco estudiado. Estos resultados son alentadores, pues demuestran el potencial empleo de esta planta en el control de moluscos indeseables.

  3. Efficacy of crude extract of Aloe secundiflora against Salmonella gallinarum in experimentally infected free-range chickens in Tanzania.

    Science.gov (United States)

    Waihenya, R K; Mtambo, M M A; Nkwengulila, G; Minga, U M

    2002-03-01

    The ethnoveterinary use of Aloe species extract in free-range local chickens against fowl typhoid was investigated. Five-months-old local chickens, free of antibodies against fowl typhoid were used. The chickens were randomly assorted into five groups including pretreated and infected (G1, n=21), infected and untreated (G2, n=21), infected and treated (G3, n=21), untreated and uninfected (G4, n=10) and treated uninfected (G5, n=10). Groups 1, 2 and 3 were inoculated with 5.0 x 10(8) c.f.u/ml of Salmonella gallinarum, following which the chickens were monitored for 15 days. There was a delay on the occurrence of the clinical signs and reduced severity of the disease in the Aloe treated chickens (G1 and G3). The mortality rates were 23.8% (5/21) in G1 (pretreated and infected), 42.8% (9/21) in G2 (infected and untreated) and 14.2% (3/21) in G3 (infected and treated). Antibody levels were raised among the infected and untreated group (G2) while they remained relatively low in the Aloe treated groups (G1 and G3). However, there was a sharp increase in the levels of interleukin 6 (IL-6) in the Aloe treated groups (G1 and G3) as compared to the untreated group (G2) until day 9 post-infection. The results of this work indicate that the extract of Aloe secundiflora may be used in the control of fowl typhoid in chickens. Studies to determine the active ingredients of the plant extract are in progress.

  4. Evaluation of Isolated Fractions of Aloe vera Gel Materials on Indinavir Pharmacokinetics: In vitro and in vivo Studies.

    Science.gov (United States)

    Wallis, Lonette; Malan, Maides; Gouws, Chrisna; Steyn, Dewald; Ellis, Suria; Abay, Efrem; Wiesner, Lubbe; Otto, Daniel P; Hamman, Josias

    2016-01-01

    Aloe vera is a plant with a long history of traditional medicinal use and is consumed in different products, sometimes in conjunction with prescribed medicines. A. vera gel has shown the ability to modulate drug absorption in vitro. The aim of this study was to fractionate the precipitated polysaccharide component of A. vera gel based on molecular weight and to compare their interactions with indinavir pharmacokinetics. Crude polysaccharides were precipitated from a solution of A. vera gel and was fractionated by means of centrifugal filtration through membranes with different molecular weight cut-off values (i.e. 300 KDa, 100 KDa and 30 KDa). Marker molecules were quantified in the aloe leaf materials by means of nuclear magnetic resonance spectroscopy and the average molecular weight was determined by means of gel filtration chromatography linked to multi-angle-laser-light scattering and refractive index detection. The effect of the aloe leaf materials on the transepithelial electrical resistance (TEER) of Caco-2 cell monolayers as well as indinavir metabolism in LS180 cells was measured. The bioavailability of indinavir in the presence and absence of the aloe leaf materials was determined in Sprague-Dawley rats. All the aloe leaf materials investigated in this study reduced the TEER of Caco-2 cell monolayers, inhibited indinavir metabolism in LS 180 cells to different extents and changed the bioavailability parameters of indinavir in rats compared to that of indinavir alone. These indinavir pharmacokinetic modulation effects were not dependent on the presence of aloverose and also not on the average molecular weight of the isolated fractions.

  5. Prevention of tri-nitrobenzene of sulfonic acid-induced colitis in chicken by using extract of Aloe vera

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    Motamed Elsayed Mahmoud

    Full Text Available Aim: Aloe vera, species of succulent plant in the genus Aloe, has multiple clinical activities and used routinely to accelerate wound healing. The present study was designed to investigate the anti-inflammatory effect of Aloe vera extracts (AVE in vitro and in vivo. Materials and Methods: The effect of crude AVE on inducible nitric oxide production by LPS/IFNg-stimulated cultured macrophages was evaluated. The therapeutic effect of administering crude Aloe vera extracts (100 mg/kg b.w. on the development of tri-nitrobenzene of sulfonic acid (TNBS-induced colitis (40 mg/kg b. w. in chicken was also investigated. Chicken is a valuable model for this purpose because it showed preference to bitter taste of Aloe vera. Diverse clinical pictures of the colitis including weight loss, diarrhea and histopathological changes were evaluated. Results: Nitrite production by LPS/IFNg-stimulated macrophages was maximally reduced by adding of AVE (100 μg/ml. This result suggests a direct inhibitory effect of AVE on the inflammatory cells. Chicks treated orally with AVE showed improvement of the histological signs with no inflammatory cell infiltrates and reduction of myeloperoxidase (MPO activities when compared with colitis control group. AVE pretreatment ameliorated significantly the clinical and histopathological severity of the TNBS-induced colitis; decreased body weight loss and diarrhea and increased survival. Conclusion: It was concluded that oral administration of AVE represents a valuable therapeutic approach for the treatment of colitis in chicken. [Vet. World 2012; 5(8.000: 469-476

  6. Vigilancia del dengue basada en el laboratorio: diferencias en el número de casos y virus aislados según la recolección del suero y la prueba serológica

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    Luis Ángel Villar

    2005-06-01

    Full Text Available Background: Dengue control measures depend on the laboratory-based surveillance data. In this study was investigated the differences in dengue cases and viruses isolated in according with surveillance strategy. Study design and methods: Two surveillance strategies were analyzed in this study. First (Local Surveillance System: single serum sample (acute or convalescent was collected from 337 dengue suspected cases and then kept at 4oC. IgM antibodies were detected using UMELISA DENGUE kit (IPK, Cuba, and serotypes identification was performed by viral isolation attempts in C6/36. Second: paired serum sample were collected from 318 dengue suspected cases and then kept at -70 ºC. IgM antibodies were detected using MAC-ELISA test and virus isolation as above. Some paired sera analyzed by MAC-ELISA were processed by “Dengue IgM-capture ELISA” kit (PANBIO and “IgM*ELISA anti-dengue” (IPK, Cuba. Results: More IgM+ dengue cases were detected by the first than second strategy using acute or convalescent serum: 61.1 vs 22% (p<0.001 y 86.8 vs 49.3% (p<0.001, respectively. Dengue diagnosis by MAC-ELISA was concordant in 85% (K=0.29 respect to UM-ELISA, but 100% (K=1 and 90.4% (K=0.84 respect to “IgM ELISA anti-dengue” and “Dengue IgM-capture ELISA” kits. Differences in dengue cases detected by the second strategy were found when serological result with single acute or paired samples was compared: 70 (22% vs 155 (48.7% (p<0.001. Dengue virus was isolated more frequently from sera kept at -70 ºC than those kept at 4 ºC: 17.8% vs. 4.7% (p<0.001. All virus serotypes were isolated. The UMELISA DENGUE test specificity and relationship between impact dengue surveillance and implemented strategy are analyzed. Conclusions: The specificity of UMELISA DENGUE test require to be evaluated by the high frequency of dengue in suspected cases. Serum paired analyzes in dengue surveillance is necessary to obtain reliable information.

  7. Evaluación del potencial biofertilizante de bacterias diazótrofas aisladas de suelos con cultivo de cacao (Theobroma cacao L.

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    Adriana Zulay Argüello-Navarro

    2014-09-01

    Full Text Available La adaptabilidad del cacao (Theobroma cacao L. en condiciones adversas permite sugerir que la microbiota asociada con la rizosfera desempeña una función importante para la nutrición y el desarrollo de la planta. En el trabajo se evaluó el potencial biofertilizante de bacterias fijadoras de nitrógeno (diazótrofas aisladas de suelos cacaoteros por la técnica de diluciones seriadas. Las bacterias se aislaron en medios semiselectivos (NFb, JMV, LGI, JNFb. Los aislados puros se caracterizaron morfológica y bioquímicamente y complementariamente se evaluó la capacidad de fijación biológica de nitrógeno (FBN por el método de Micro-Kjeldahl. Los aislados con mejor capacidad de fijación de nitrógeno fueron seleccionados para evaluar su potencial promotor de crecimiento durante 120 días en plantas de cacao clon CCN 51 establecidas a partir de semillas. El análisis de datos mostró que las plantas de cacao respondieron efectivamente a los tratamientos con bacterias donde las variables de crecimiento y N (% foliar incrementaron significativamente, en comparación con el testigo. Se destacó el aporte de los aislados DKA2J24, DKB3J73 presuntamente del género Burkholderia sp. y DKA1L3 semejante al género Gluconacetobacter sp., todos con un gran potencial como biofertilizantes en la prueba de bioensayo.

  8. Efectos de la substitución de conexina 43 por conexina 32 en la tolerancia a la isquemia y en la protección por precondicionamiento en corazones aislados de ratón

    OpenAIRE

    2011-01-01

    La conexina 43 es una proteína que juega un papel importante en la fisiopatología de la isquemia - reperfusión y en la señalización de la protección cardíaca. Para investigar si este papel está relacionado con las propiedades específicas de esta isoforma, se utilizó un modelo de corazón aislado de ratones transgénicos knock-in, en los que la conexina 43 se reemplazó por conexina 32. Los resultados de este trabajo demuestran un efecto modulador de la conexina 43 en el metabolismo energético, e...

  9. Effect of packaging methods and crude aloe vera gel (Aloe vera barbadensis miller) on the storage quality of enrobed goat meat bites.

    Science.gov (United States)

    Jairath, Gauri; Chatli, Manish K; Sahoo, Jhari; Biswas, Ashim K

    2015-06-01

    The storage stability of enrobed goat meat bites (EGMB) incorporated with 3 % crude aloe vera (AV) gel was evaluated under aerobic (T-1; unenrobed control product, T-2; enrobed AV treated product) and modified atmospheric packaging (MAP, 50:50, CO2 and N2) (T-3; unenrobed control product, T-4; enrobed AV treated product) at 4 ± 1 °C for 42 days on the basis of physico-chemical, microbiological and sensory attributes. The pH value was higher, whereas water activity (aW) was lower in enrobed and MAP product. Thiobarbituric acid reacting substances (TBARS) and free fatty acid (FFA) values were significantly (P Standard Plate Count (SPC) was significantly (P < 0.05) lower in MAP products than aerobic packaged products. Results concluded that EGMB treated with AV gel can be successfully stored more than 42 days under MAP conditions without affecting its physico-chemical, textural, microbiological and sensory attributes.

  10. 芦荟库拉索(Aloe vera L)再生体系的建立%Establishment of Plant Regeneration System for Aloe vera L.

    Institute of Scientific and Technical Information of China (English)

    吴英; 郭安平; 孔华; 贺立卡; 郭运玲

    2006-01-01

    对芦荟(Aloe vera L.)组织培养和再生体系进行了研究,初步获得芦荟的初代和继代培养基为MS+6-BA2.5 mg/L+NAA 0.2 mg/L+蔗糖30 mg/L,生根培养基成分为1/2MS+IBA 0.2 mg/L+NAA 0.1 mg/L+蔗糖30 mg/L,愈伤组织培养采用粗壮的芦荟基部的白色部分,诱导愈伤组织的培养基为B5+6-BA 2.0 mg/L+2,4-D 2.0 mg/L+蔗糖30 mg/L,采用Vc 5.0 mg/L抑制愈伤组织诱导过程中褐变的产生.诱导愈伤组织和外植体直接出芽2种方式并存.试管苗移栽后存活率为100%.采用潮霉素为抗性筛选标记,选择浓度为20~30 mg/L.

  11. EFFECT OF DIETARY SUPPLEMENTATION OF Melissa Officinalis and Aloe Vera ON HEMATOLOGICAL TRAITS, LIPID OXIDATION OF CARCASS AND PERFORMANCE IN RAINBOW TROUT (Oncorhynchus Mykiss

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    A. FARAHI

    2012-02-01

    Full Text Available This was conducted investigate the effect of feeding lemon balm (Melissa officinalis and Aloe (Aloe vera on growth performance, hematological parameters and oxidative stability of rainbow trout. 360 uniform rainbow trout (20.87±0.25 g were divided into 3 groups, and fed standard diets supplemented with ground lemon balm (2%, L group or supplemented with Aloe (1%, A group and without supplementation (Control, C group. Growth performance and body composition were not influenced by plant supplementation. Survival rate of fish was promoted in diets supplemented with herbs, significantly (P0.05 were not observed in RBC and Hb in treatments (P>0.05. Results of thiobarbituric acid value (TBA showed that lemon balm and Aloe herbs could be protective against lipid peroxidation in fish meat during chilling storage (4°C, 7 days.

  12. Resistencia antimicrobiana en aislados de "Escherichia coli" de conejos tratados por vía oral con diferentes pautas de doxiciclina

    OpenAIRE

    Medina Landaeta, Alberto Enrique

    2012-01-01

    La resistencia a antimicrobianos constituye actualmente un problema de gran impacto en Salud Pública. El excesivo uso de antimicrobianos en medicina humana y veterinaria y en agricultura ha originado un incremento continuo de fracasos en la terapia antimicrobiana debido al aumento y diversidad de microorganismos resistentes. La evaluación del riesgo del desarrollo de resistencias cobra especial interés cuando se utilizan pautas de administración por vía oral en animales de abasto, por cuan...

  13. El derecho penal como ciencia unitaria: Una respuesta al conflicto entre el saber dogmático aislado formal y el saber disciplinar e interdisciplinar

    OpenAIRE

    Jaime Sandoval Fernández

    2012-01-01

    El derecho penal como ciencia unitaria está integrado por la dogmática, la criminología y la política criminal. La dogmática de nuestro tiempo debe ser teleológica. Está al servicio del Estado constitucional de derecho y propende reducir o contener la violencia intolerablemente irracional del poder punitivo. Este derecho penal como ciencia unitaria interactúa con otros saberes jurídicos y no jurídicos, dando lugar a relaciones disciplinares e interdisciplinares. La dogmática jurídico-penal y ...

  14. Cirugía de reemplazo valvular aórtico aislado en pacientes octogenarios: evaluación, riesgo operatorio y resultados a mediano plazo

    OpenAIRE

    Fernando Piccinini; Juan Vrancic; Guillermo Vaccarino; Hernán Raich; Gustavo Siles; Mariano Benzadón; Jorge Thierer; Daniel Navia

    2010-01-01

    RESUMENIntroducciónLas indicaciones y los beneficios del reemplazo valvular aórtico han sido claramente establecidos, aun para poblaciones añosas, de aumentada comorbilidad. Aun así, la manipulación de ateromas aórticos, el uso de circulación extracorpórea y de clampeo aórtico, el paro cardíaco y la descalcificación ligadas a esta técnica y sus eventuales consecuencias generan dudas acerca de su indicación en este grupo de pacientes.ObjetivosComunicar la morbimortalidad del procedimiento en o...

  15. Efectos de la insulina y el agua oxigenada sobre el metabolismo de los hidratos de carbono en adipocitos aislados de tejido adiposo de rata

    OpenAIRE

    Padial Le??n, Mar??a Jos??

    1990-01-01

    Se ha realizado un estudio comparativo de los efectos de la insulina y el h2o2 tanto en tejido adiposo de ratas normales como en tejido adiposo de ratas sometidas a un periodo prolongado de ayuno (situaci??n que hay que resaltar se manifiesta por una alteraci??n de la respuesta biol??gica a la insulina), en dicho estudio se incluye la observaci??n del efecto de la insulina y el per??xido de hidrogeno sobre la actividad de un enzima clave del metabolismo intermediario que sea diana de la activ...

  16. Effects of plant sterols derived from Aloe vera gel on human dermal fibroblasts in vitro and on skin condition in Japanese women

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    Tanaka M

    2015-02-01

    Full Text Available Miyuki Tanaka,1 Eriko Misawa,1 Koji Yamauchi,1 Fumiaki Abe,1 Chiaki Ishizaki2 1Functional Food Research Department, Food Science and Technology Institute, Morinaga Milk Industry Co, Ltd, Zama, Kanagawa, 2Ebisu Skin Research Center, Inforward, Inc., Tokyo, Japan Background: Aloe is known for its topical use for treating wounds and burns. Many previous studies reported the healing effects of Aloe vera. However, there are few clinical studies on the effect of orally administered A. vera gel on the skin. Aloe sterols are a type of plant sterols that have the capability to regulate the metabolism of glucose and lipids. In a recent study, we confirmed that ingested Aloe sterols reached the peripheral tissues through the bloodstream. However, their influence on dermal fibroblasts has not been investigated. Methods: First, we investigated the capability of Aloe sterols (cycloartenol and lophenol to stimulate human dermal fibroblasts in vitro. Then, we investigated the effect of intake of Aloe vera gel powder (AVGP containing 40 µg Aloe sterols on the skin conditions in Japanese women with dry skin in a randomized, double-blind, placebo-controlled trial. Results: After cocultivation with Aloe sterols, the production of collagen and hyaluronic acid increased by approximately two-fold and 1.5-fold, and gene expression levels of these enzymes responsible for their synthesis were also observed in human dermal fibroblasts. An increase in arm skin hydration was observed at 8 weeks in the AVGP group, whereas a slight decrease in arm skin hydration was noted in the placebo group. However, there was no statistical difference between AVGP and placebo groups in skin moisture. In subgroup analysis, the change in the mean wrinkle depth was significantly lower in the AVGP group than in the control group. In addition, percent body fat after 8 weeks was significantly lower in the AVGP group. No AVGP intake-dependent harmful phenomenon was observed during the intake

  17. PRELIMINARY RESULTS OF ESTIMATING SOIL MOISTURE OVER BARE SOIL USING FULL-POLARIMETRIC ALOS-2 DATA

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    A. Sekertekin

    2016-10-01

    Full Text Available Synthetic Aperture Radar (SAR imaging system is one of the most effective way for Earth observation. The aim of this study is to present the preliminary results about estimating soil moisture using L-band Synthetic Aperture Radar (SAR data. Full-polarimetric (HH, HV, VV, VH ALOS-2 data, acquired on 22.04.2016 with the incidence angle of 30.4o, were used in the study. Simultaneously with the SAR acquisition, in-situ soil moisture samples over bare agricultural lands were collected and evaluated using gravimetric method. Backscattering coefficients for all polarizations were obtained and linear regression analysis was carried out with in situ moisture measurements. The best correlation coefficient was observed with VV polarization. Cross-polarized backscattering coefficients were not so sensitive to soil moisture content. In the study, it was observed that soil moisture maps can be retrieved with the accuracy about 14% (RMSE.

  18. Nitric oxide production by chicken macrophages activated by Acemannan, a complex carbohydrate extracted from Aloe vera.

    Science.gov (United States)

    Karaca, K; Sharma, J M; Nordgren, R

    1995-03-01

    Cultures of normal chicken spleen cells and HD11 line cells produce nitric oxide (NO) in response to Acemannan, a complex carbohydrate derived from the Aloe vera plant. Neither cell type produced detectable amounts of NO in response to similar concentrations of yeast mannan, another complex carbohydrate. Nitric oxide production was dose dependent and inhibitable by the nitric oxide synthase inhibitor NG-methyl-L-arginine. In addition, the production of NO was inhibited by preincubation of ACM with concanavalin A in a dose-dependent manner. These results suggest that ACM-induced NO synthesis may be mediated through macrophage mannose receptors, and macrophage activation may be accountable for some of the immunomodulatory effects of ACM in chickens.

  19. Optimization of the preparation of pectin from Aloe using a Box-Behnken design.

    Science.gov (United States)

    Geng, Lijing; Zhou, Wei; Qu, Xingyuan; Chen, Weijie; Li, Yingchang; Liu, Chang; Sun, Jing; Yu, Xiaolei; Wang, Hui; Zhang, Zhen; Li, Jing; Wang, Lijie

    2014-05-25

    The extraction condition of pectin from Aloe vera barbadensis Mill was optimized by a Box-Behnken design. The effect of parameters of extraction water proportion (EWP), extraction pH (EpH), extraction temperature (ETe), extraction time (ETi), alcohol precipitation pH (APpH)and alcohol precipitation temperature (APTe) on the extraction yield of pectin was investigated by a software of Design Export 8.0.5b. The maximum extraction yield was obtained with the EWP of 20:1, EpH of 1.5, ETe of 90°C, ETi of 120min, APpH of 3.0 and APTe of 50°C, which was consistent with the experimental value. We also found out that the pectin content decreased gradually during storage and sucrose concentration had a significant impact on the viscosity of pectin.

  20. Encapsulation of Aloe Vera extract into natural Tragacanth Gum as a novel green wound healing product.

    Science.gov (United States)

    Ghayempour, Soraya; Montazer, Majid; Mahmoudi Rad, Mahnaz

    2016-12-01

    Application of natural materials in wound healing is an interest topic due to effective treatment with no side effects. In this paper, Aloe Vera extract was encapsulated into Tragacanth Gum through a sonochemical microemulsion process to prepare a wound healing product. FESEM/EDX and FT-IR proved the successfully formation of the nanocapsules with spherical shape by cross-linking aluminum ions with Tragacanth Gum. The therapeutic characteristics of the prepared wound healing product were investigated using antimicrobial, cytotoxicity and wound healing assays. Relative high antimicrobial activities with the microbial reduction of 84, 91 and 80% against E. coli, S. aureus and C. albicans, a cell viability of 98% against human fibroblast cells and a good wound healing activity with considerable migration rate of fibroblast cells are the important advantages of the new formed wound healing product.

  1. Effect of vehicles on topical application of aloe vera and arnica montana components.

    Science.gov (United States)

    Bergamante, Valentina; Ceschel, Gian Carlo; Marazzita, Sergio; Ronchi, Celestino; Fini, Adamo

    2007-10-01

    In this study two types of gels and microemulsions are investigated for their ability to dissolve, release, and induce the permeation of helenalin, a flavonoid responsible for the anti-inflammatory activity of arnica montana extract, and aloin, an anthrone-C-glucosyls with antibacterial activity present in aloe vera extract. The release of these agents from each vehicle was followed by HPLC, and transcutaneous permeation was examined using a modified Franz cell and a porcine skin membrane. The study showed that a microemulsion can be a good vehicle to increase the permeation of helenalin, while the gel formulation, containing Sepigel 305, proved able to reduce the release and permeation of aloin, with a consequent activity limited to the surface of application, without any permeation. This is in accordance with the necessity to avoid this process, since human skin fibroblasts can metabolize absorbed aloin into a structurally related compound that increases the sensitivity of skin to ultraviolet light.

  2. Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation.

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    Yun Sun Lee

    Full Text Available Aloe vera (Asphodeloideae is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10-11 and 5-13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment.

  3. Dietary aloe vera gel powder and extract inhibit azoxymethane- induced colorectal aberrant crypt foci in mice fed a high- fat diet.

    Science.gov (United States)

    Chihara, Takeshi; Shimpo, Kan; Kaneko, Takaaki; Beppu, Hidehiko; Higashiguchi, Takashi; Sonoda, Shigeru; Tanaka, Miyuki; Yamada, Muneo; Abe, Fumiaki

    2015-01-01

    Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.

  4. Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress

    Directory of Open Access Journals (Sweden)

    Fábio Sabbadin Zanuzzo

    2012-10-01

    Full Text Available This study evaluated the effect of extract of Aloe vera in the transport water of matrinxã (Brycon amazonicus fish on stress response and leukocyte respiratory activity. Fish was transported for 4 h in water containing Aloe at levels 0; 0.02; 0.2 and 2 mg/L, and sampled before transport 2, 4, 24 and 96 h after for determination of plasma glucose and respiratory activity of leukocytes. An additional in vitro assay was conducted with another fish species, pacu (Piaractus mesopotamicus, to test the respiratory burst of leukocytes exposed to Aloe extract (0.0, phosphate-buffered saline (PBS only at 0.1, 0.2, 0.5 and 1 mg/L. Plasma glucose increased after 2 and 4 h of transport and returned to control levels within 24 h, but the addition of Aloe in the transport water did not affect the level of blood glucose. However, at 2 h of transport, Aloe enhanced the respiratory activity of leukocytes in a dose-dependent way. The highest value of respiratory burst activity of leukocytes was observed in the fish transported in water containing Aloe at 2 mg/L. The enhancing effect of the plant extract on the production of oxygen radicals was confirmed in vitro in leukocytes of pacu incubated in Aloe at concentrations 0.1 and 0.2 mg/L. The results suggest that Aloe vera is a modulator of the immune system in fish improving the innate immune response tested.

  5. Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge.

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; Ma, Xin Yu; He, Jie; Xu, Pao; Liu, Kai

    2015-10-01

    The current study investigated the effects of dietary Aloe vera on plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities of GIFT-tilapia juveniles under Streptococcus iniae challenge. Five dietary groups were designed including a control and 100 % Aloe powder incorporated into a tilapia feed at 0.5, 1, 2, and 4 %/kg feed, which were administered for 8 weeks. Fish fed dietary Aloe at 4 %/kg feed significantly reduced in total cholesterol, while triacylglycerol reduced (P Aloe/kg feed compared to unsupplemented ones. High-density lipoprotein was significantly elevated in fish fed 0.5 and 1 % Aloe/kg feed compared to unsupplemented ones, and no significant changes (P > 0.05) were noted in low-density lipoprotein among test groups. Furthermore, high activities of superoxide dismutase, catalase, and glutathione peroxide in liver tissues were observed in Aloe-supplemented fish compared to unsupplemented ones, before and after S. iniae challenge (7.7 × 10(6) CFU cells/mL). Variations were also noted in malondialdehyde activity throughout the trial, but no significant difference (P > 0.05) was observed between groups. Meanwhile, Aloe-supplemented fish reduced serum aspartate and alanine aminotransferase (AST and ALT) activities before and after challenge. Based on the second-order polynomial regression analysis, dietary Aloe inclusion levels less than or equal to 1.88, 1.86, and 2.79 %/kg feed were determined to be suitable in improving plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia in this study, respectively. Thus, A. vera extracts may be recommended as a tilapia feed supplement to enhance fish antioxidant and hepatoprotective capacities, especially during disease outbreaks.

  6. Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation.

    Science.gov (United States)

    Lee, Yun Sun; Ju, Hyun Kyoung; Kim, Yeon Jeong; Lim, Tae-Gyu; Uddin, Md Romij; Kim, Yeon Bok; Baek, Jin Hong; Kwon, Sung Won; Lee, Ki Won; Seo, Hak Soo; Park, Sang Un; Yang, Tae-Jin

    2013-01-01

    Aloe vera (Asphodeloideae) is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10-11 and 5-13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment.

  7. Co-expression of NCED and ALO improves vitamin C level and tolerance to drought and chilling in transgenic tobacco and stylo plants.

    Science.gov (United States)

    Bao, Gegen; Zhuo, Chunliu; Qian, Chunmei; Xiao, Ting; Guo, Zhenfei; Lu, Shaoyun

    2016-01-01

    Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses, while L-ascorbic acid (AsA) that is also named vitamin C is an important antioxidant and involves in plant stress tolerance and the immune system in domestic animals. Transgenic tobacco (Nicotiana tabacum L.) and stylo [Stylosanthes guianensis (Aublet) Swartz], a forage legume, plants co-expressing stylo 9-cis-epoxycarotenoid dioxygenase (SgNCED1) and yeast D-arabinono-1,4-lactone oxidase (ALO) genes were generated in this study, and tolerance to drought and chilling was analysed in comparison with transgenic tobacco overexpressing SgNCED1 or ALO and the wild-type plants. Compared to the SgNCED1 or ALO transgenic plants, in which only ABA or AsA levels were increased, both ABA and AsA levels were increased in transgenic tobacco and stylo plants co-expressing SgNCED1 and ALO genes. Compared to the wild type, an enhanced drought tolerance was observed in SgNCED1 transgenic tobacco plants with induced expression of drought-responsive genes, but not in ALO plants, while an enhanced chilling tolerance was observed in ALO transgenic tobaccos with induced expression of cold-responsive genes, but not in SgNCED1 plants. Co-expression of SgNCED1 and ALO genes resulted in elevated tolerance to both drought and chilling in transgenic tobacco and stylo plants with induced expression of both drought and cold-responsive genes. Our result suggests that co-expression of SgNCED1 and ALO genes is an effective way for use in forage plant improvement for increased tolerance to drought and chilling and nutrition quality.

  8. Utilization of plant bioactives as feed additives for poultry: The effect of Aloe vera gel and its extract on performance of broilers

    OpenAIRE

    A.P Sinurat; T Purwadaria; M.H Togatorop; T Pasaribu

    2003-01-01

    Feed additives are commonly added in poultry feed as a growth promotant or to improve feed efficiency. The most common feed additive used is antibiotic at sub-therapheutic doses, although there is a controversy on its impact on human health. Previous results showed that Aloe vera gel could improve feed efficiency in broilers and an in vitro study showed that the extract have an antibacterial effect. Therefore, a further experiment was designed to study the response of broilers to Aloe vera ge...

  9. Characterization of mangrove species using ALOS-2 PALSAR in Hai Phong city, Vietnam

    Science.gov (United States)

    Dat Pham, Tien; Yoshino, Kunihiko

    2016-06-01

    Hai Phong city is located in the Northern coast of Vietnam where the mangroves are distributed between zone I and zone II among the four mangrove zones in Vietnam. This city is vulnerable to rising sea levels associated with climate change and tropical cyclones, which are forecasted to become more severe due to the impact of climate change. In the past, mangrove forests in this city have decreased markedly because of over expansion of shrimp farming. Thus, identification of mangrove forests is important to monitor and support mangrove conservation and management in the coastal zone. The objectives of this research were to identify the locations of mangrove and characterize mangrove species in Hai Phong using HH and HV backscatters of the Advanced Land Observing Satellite 2 (ALOS-2) with enhanced Phased Array L-band Synthetic Aperture Radar (PALSAR). Image segmentation was used to identify the locations of mangrove forests. Moreover, Geographic Information System (GIS) was applied to update current status of mangrove species in 2015. The results showed that the means of HH and HV backscatter coefficients of K. obovata are lower than S. caseolaris. K. obovata has HH value around -13.9 dB until -10.3 dB and HV value around -20.6 dB until -16.2 dB. Higher HH values between about -14.9 dB and -6.8 dB and HV values between roughly -20.6 dB and -14.3 dB have observed by S. caseolaris. The total area of mangrove forest in Hai Phong in the year 2015 was around 4084 hectares, of which S. caseolaris occupied over 68% and mixed mangrove species was approximately 25.6%. This research indicates the potential for the use of L-band ALOS-2 PALSAR in characterizing mangrove forest species in the coastal zone.

  10. A Modified Hopfield Neural Network Algorithm (MHNNA Using ALOS Image for Water Quality Mapping

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    Ahmed Asal Kzar

    2015-12-01

    Full Text Available Decreasing water pollution is a big problem in coastal waters. Coastal health of ecosystems can be affected by high concentrations of suspended sediment. In this work, a Modified Hopfield Neural Network Algorithm (MHNNA was used with remote sensing imagery to classify the total suspended solids (TSS concentrations in the waters of coastal Langkawi Island, Malaysia. The adopted remote sensing image is the Advanced Land Observation Satellite (ALOS image acquired on 18 January 2010. Our modification allows the Hopfield neural network to convert and classify color satellite images. The samples were collected from the study area simultaneously with the acquiring of satellite imagery. The sample locations were determined using a handheld global positioning system (GPS. The TSS concentration measurements were conducted in a lab and used for validation (real data, classification, and accuracy assessments. Mapping was achieved by using the MHNNA to classify the concentrations according to their reflectance values in band 1, band 2, and band 3. The TSS map was color-coded for visual interpretation. The efficiency of the proposed algorithm was investigated by dividing the validation data into two groups. The first group was used as source samples for supervisor classification via the MHNNA. The second group was used to test the MHNNA efficiency. After mapping, the locations of the second group in the produced classes were detected. Next, the correlation coefficient (R and root mean square error (RMSE were calculated between the two groups, according to their corresponding locations in the classes. The MHNNA exhibited a higher R (0.977 and lower RMSE (2.887. In addition, we test the MHNNA with noise, where it proves its accuracy with noisy images over a range of noise levels. All results have been compared with a minimum distance classifier (Min-Dis. Therefore, TSS mapping of polluted water in the coastal Langkawi Island, Malaysia can be performed using the

  11. Mapping Canopy Height and Growing Stock Volume Using Airborne Lidar, ALOS PALSAR and Landsat ETM+

    Directory of Open Access Journals (Sweden)

    Wayne Walker

    2012-10-01

    Full Text Available We have investigated for forest plantations in Chile the stand-level retrieval of canopy height (CH and growing stock volume (GSV using Airborne Laser Scanner (ALS, ALOS PALSAR and Landsat. In a two-stage up-scaling approach, ensemble regression tree models (randomForest were used to relate a suite of ALS canopy structure indices to stand-level in situ measurements of CH and GSV for 319 stands. The retrieval of CH and GSV with ALS yielded high accuracies with R2s of 0.93 and 0.81, respectively. A second set of randomForest models was developed using multi-temporal ALOS PALSAR intensities and repeat-pass coherences in two polarizations as well as Landsat data as predictor and stand-level ALS based estimates of CH and GSV as response variables. At three test sites, the retrieval of CH and GSV with PALSAR/Landsat reached promising accuracies with R2s in the range of 0.7 to 0.85. We show that the combined use of multi-temporal PALSAR intensity, coherence and Landsat yields higher retrieval accuracies than the retrieval with any of the datasets alone. Potential limitations for the large-area application of the fusion approach included (1 the low sensitivity of ALS first/last return data to forest horizontal structure, affecting the retrieval of GSV in less managed types of forest, and (2 the dense ALS sampling required to achieve high retrieval accuracies at larger scale.

  12. The Oasis impact structure, Libya: geological characteristics from ALOS PALSAR-2 data interpretation

    Science.gov (United States)

    van Gasselt, Stephan; Kim, Jung Rack; Choi, Yun-Soo; Kim, Jaemyeong

    2017-02-01

    Optical and infrared remote sensing may provide first-order clues for the identification of potential impact structures on the Earth. Despite the free availability of at least optical image data at highest resolution, research has shown that remote sensing analysis always remains inconclusive and extensive groundwork is needed for the confirmation of the impact origin of such structures. Commonly, optical image data and digital terrain models have been employed mainly for such remote sensing studies of impact structures. With the advent of imaging radar data, a few excursions have been made to also employ radar datasets. Despite its long use, capabilities of imaging radar for studying surface and subsurface structures have not been exploited quantitatively when applied for the identification and description of such features due to the inherent complexity of backscatter processes. In this work, we make use of higher-level derived radar datasets in order to gain clearer qualitative insights that help to describe and identify potential impact structures. We make use of high-resolution data products from the ALOS PALSAR-1 and ALOS PALSAR-2 L-band sensors to describe the heavily eroded Oasis impact structure located in the Libyan Desert. While amplitude radar data with single polarization have usually been utilized to accompany the suite of remote sensing datasets when interpreting impact structures in the past, we conclude that the integration of amplitude data with HH/HV/HH-HV polarization modes in standard and, in particular, in Ultra-Fine mode, as well as entropy-alpha decomposition data, significantly helps to identify and discriminate surface units based on their consolidation. Based on the overarching structural pattern, we determined the diameter of the eroded Oasis structure at 15.6 ± 0.5 km.

  13. Healing effect of aloe vera gel,in non-healed ulcers

    Institute of Scientific and Technical Information of China (English)

    Majid Avijgan; Victoria Beigi Broujeni; Ali Akbar Beigi; Hojatollah Rohi Borojeni; Seyyed Ka-myar Mostafavizadeh; Iraj Karimi; Abbas Ali Javadi

    2009-01-01

    Objective:Aloe Vera gel (AVG)has a prominent role as a contemporary folk remedy.The Non-healed chron-ic ulcers of 16 non-responders to conventional treatment were treated with AVG for which the clinical efficacy was studied.Methods:This study included 16 patients having chronic ulcer (9 males and 7 females)with mean age of 41.8 ±14.6 who following failure treatment to conventional therapy,were dispensed AVG to ap-ply.AVG is consisted from gel part of Aloe Vera plant plus one preservative material,in this study ECG jelly. The patients were assessed as responder whenever intensity of inflammation,the number and /or the size of ul-cers have been reduced.Results:The period of time till healing (complete or reduction in size of ulcer), were from 5 to 160 days with mean of 31.2 ±36.4 days.The mean time of decreasing for secreting,swelling and redness was from 5-11 days.Spearman coefficient of correlation,showed no significant correlation between first size and depth of ulcer and timing till 50% reduction in size of ulcer.Conclusion:All patients had been treated with conventional treatment all to no avail.There were some full thickness ulcer which at the end of treatment we observed an improvement and normal skin for those ulcers.There was a minor complication like itching in one patient but no leading to stop treatment.These findings suggest that AVG is an excellent and sa-fer choice than current conventional therapeutic management of chronic ulcers.

  14. Aloe Vera

    Science.gov (United States)

    63053 ... https://nccih.nih.gov/health/aloevera ... us ... 63053 ... https://nccih.nih.gov/health/aloevera ... Burns ... Burns/NON-DISPLAY (Supplements page) ... us ... 63053 ... https://nccih.nih. ...

  15. Vasodilator profile of flavonoid and phenylbutanoid compounds isolated from Croton schiedeanus Schlecht PERFIL VASODILATADOR DE COMPUESTOS FLAVONOIDES Y FENILBUTANOIDES AISLADOS DE CROTON SCHIEDEANUS SCHLECHT

    Directory of Open Access Journals (Sweden)

    Sofía Ximena Correa-Hernández

    2008-12-01

    para el tratamiento de la hipertensión arterial. Objetivo. Evaluar el efecto vasodilatador de los flavonoides: 3-O-metilquercetina, 3,7-di-O-metilquercetina, y 3,3',4',7-tetra-O-metilquercetina; y los fenilbutanoides: (2S-7,9-dime--to--xiro---doden-drol, (2S-2-acetato de 7,9-dime-toxirododendrol y (2S- 2,8-diacetato de 7,9-dimetoxirodo-dendrol en anillos de aorta de ratas Wistar. Material y métodos. Estos compuestos se evaluaron en anillos de aorta precontraídos con fenilefrina (1 µM o KCl (80 mM. Para examinar posibles interacciones con endotelio, óxido nítrico, guanilato ciclasa, prostanoides o canales de K+ATP, aquéllos con mayores efectos vasodilatadores: 3-O-metilquercetina y 3,7-di-O-metilquercetina, se evaluaron en anillos estimulados con fenilefrina en presencia o ausencia de: endotelio, L-NAME (G -nitro-L-Arginina-Metil Ester, 100 µM, ODQ (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-ona, 1 µM, meclofenamato sódico (10 µM o glibenclamida (1 µM. Resultados. En contraste con los compuestos fenilbutanoides que no arrojaron actividad relajante significativa, 3-O-metilquercetina y 3,7-di-O-metilquercetina mostraron una respuesta importante con concentraciones inhibitorias 50 (CI50 de 2,5 y 4,9 µM respectivamente frente a fenilefrina. ODQ y L-NAME desplazaron efectivamente a la derecha la curva dosis-respuesta, en particular la 3-O-metilquercetina (razón de IC50: 7,4 y 3,8. Conclusión. 3-O-metilquercetina y 3,7-di-O-metilquer-cetina, flavonoides aislados de Croton schiedeanus, ejercen importantes efectos vasodilatadores vinculados con la vía de NO/GMPc. Estos resultados soportan al uso etnobotánico de esta especie.

  16. Identificación del genotipo del virus del papiloma humano en pacientes portadoras de lesiones cérvico uterinas

    Directory of Open Access Journals (Sweden)

    Alexander Morales Fontaine

    2016-02-01

    Full Text Available Fundamento: en los últimos años se ha observado un elevado predominio de mujeres infestadas por genotipos del  Virus del Papiloma Humano considerados de alto riesgo por su elevada oncogenicidad.Objetivo: identificar los genotipos del Virus del Papiloma Humano en pacientes portadoras de este con lesiones cervicales, cuyas biopsias fueron analizadas en el departamento de anatomía patológica, perteneciente al Hospital General Docente ¨Ernesto Guevara de la Serna¨, en el período comprendido entre enero de 2013 a diciembre de 2014.Métodos: se realizó un estudio descriptivo, de corte transversal, en 45 pacientes cuyas biopsias fueron tomadas de forma aleatoria de un total de 456 casos en los que se encontraron evidencias histopatológicas de infección por el  Virus del Papiloma Humano, en el período referido período de estudio. Las biopsias fueron enviadas al Laboratorio de Biología Molecular del departamento de Virología del Instituto de Medicina Tropical ´´Pedro Kourí´, a fin de aislar e identificar los tipos de este virus, involucrados en la patogénesis de las lesiones premalignas en estudio. Se utilizaron fuentes secundarias dadas por los resultados de la identificación del genotipo del virus aislado enviados desde dicha institución. Los datos se procearon según la estadística descriptiva, mediante el análisis porcentual.Resultados: en el 88,8 % de la muestra se obtuvo infestación por el Virus del Papiloma Humano, de alto riesgo de oncogenicidad, de ellos hay una preponderancia del genotipo 16, con 26 casos (57,7 % seguido del 31 con 12 aislamientos (26,6 % y 2 pacientes presentaron el genotipo 52 para el 4,4 %. El 6,6 % de la muestra resultó estar infestadas por el genotipo 11, el cual es considerado de bajo riesgo y en 2 casos no fue posible realizar la tipificación.Conclusiones: Existió gran predominio de pacientes afectadas por Virus de Papiloma Humano de alto riesgo de oncogenicidad.

  17. Effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing in rats

    Institute of Scientific and Technical Information of China (English)

    Kallaya Eamlamnam; Suthiluk Patumraj; Naruemon Visedopas; Duangporn Thong-Ngam

    2006-01-01

    AIM: To compare the effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing.METHODS: Male Spraque-Dawley rats (n=48) were divided into four groups. Group1 served as control group,group 2 as gastric ulcer group without treatment, groups 3 and 4 as gastric ulcer treatment groups with sucralfate and Aloe vera. The rats from each group were divided into 2 subgroups for study of leukocyte adherence, TNF-α and IL-10 levels and gastric ulcer healing on days 1 and 8 after induction of gastric ulcer by 20% acetic acid. RESULTS: On day 1 after induction of gastric ulcer, the leukocyte adherence in postcapillary venule was significantly (P< 0.05) increased in the ulcer groups when compared to the control group. The level of TNF-αwas elevated and the level of IL-10 was reduced. In the ulcer groups treated with sucralfate and Aloe vera,leukocyte adherence was reduced in postcapillary venule.The level of IL-10 was elevated, but the level of TNF-αhad no significant difference. On day 8, the leukocyte adherence in postcapillary venule and the level of TNF-αwere still increased and the level of IL-10 was reduced in the ulcer group without treatment. The ulcer treated with sucralfate and Aloe vera had lower leukocyte adherence in postcapillary venule and TNF-α level. The level of IL-10 was still elevated compared to the ulcer group without treatment. Furthermore, histopathological examination of stomach on days 1 and 8 after induction of gastric ulcer showed that gastric tissue was damaged with inflammation. In the ulcer groups treated with sucralfate and Aloe vera on days 1 and 8, gastric inflammation was reduced, epithelial cell proliferation was enhanced and gastric glands became elongated. The ulcer sizes were also reduced compared to the ulcer group without treatment.CONCLUSION: Administration of 20% acetic acid can induce gastric inflammation, increase leukocyte adherence in postcapillary venule and TNF-α level

  18. Development of Aloe Capsule with Defaecation Function%通便功能芦荟胶囊的研制

    Institute of Scientific and Technical Information of China (English)

    刘华

    2012-01-01

    Objective To develop aloe capsule with defaecation function and establish the quality standard of the product. Methods The formula of aloe capsule was screened by the preparation research. The production process was designed and the safety, stability and the defaecation function were assessed for aloe capsule. Results The optimum formula was as follows: aloe freeze-dried powder( on dried basis) about 1.5g/d, soy dietary fiber 90-100 mg/d, corn starch 1200-1400 mg/d, silicon dioxide 3.8-4.1 mg/d. Three consecutive batches were stable in quality, and the functional composition, physical and chemical indices presented good stability for three months at 38+ 1 ℃ and 75% RH. No marked toxic effect was observed. The defaecation and characteristics of feces had obvious improvement (P<0.05) . Conclusion The production process can be feasible for aloe capsule. The aloe capsule has defaecation function and good stability.%目的 研制有通便功能的芦荟胶囊,建立产品质量标准.方法 通过研究制剂成型性优化筛选富莱欣牌芦荟胶囊配方,设计生产工艺,评价产品稳定性、安全性以及通便功能.结果 确定芦荟胶囊所用芦荟冻干粉每天最佳用量折合芦荟(以干品计)约1.5 g/d,确定大豆膳食纤维最佳用量区间为90~100 mg/d,玉米淀粉最佳用量区间为1200~1400 mg/d,二氧化硅最佳添加量为3.8~4.1 mg/d.连续3批产品质量稳定,功效成分及理化指标在3个月(38±1)℃和相对湿度75%的环境中有很好的稳定性.未见明显毒副作用、排便状况和粪便性状明显改善(P<0.05).结论 该胶囊生产工艺可行,证明具有明显的通便功能,且各项质量指标稳定性良好.

  19. Formalización del lenguaje en el deterioro esquizofrénico.

    Directory of Open Access Journals (Sweden)

    Carmen Jiménez Casado

    1986-01-01

    Full Text Available El presente trabajo intenta aislar estructuras de habla comunes en el lenguaje del esquizofrénico deteriorado, con objeto de aportar criterios aproximativos que faciliten la interpretación de la dinámica concreta de este cuadro. Para ello se han empleado las respuestas dadas frente al Test de Apercepción Temática por un grupo de esquizofrénicos con deterioro, y se han comparado con las dadas por un grupo de no consultantes. Mediante la aplicación del método de análisis del lenguaje propuesto por CASTILLA DEL PINO, a partir de los presupuestos de la Teoría Hermenéutica, se tratará de poner en relación los dinamismos peculiares del esquizofrénico crónico con su forma de habla. Se han aislado nuevas variables y se concluye que el esquizofrénico residual posee una forma de hablar peculiar que difiere significativamente de la del sujeto no consultante y caracterizada, fundamentalmente, por un descenso global de la denotación y connotación.

  20. Eolic central Guerrero Negro, BCS, Mexico, performance first year operation; Central eolica Guerrero Negro, BCS, Mexico, resultados del primer ano de operacion

    Energy Technology Data Exchange (ETDEWEB)

    Cadenas Tovar, Roberto; Sanchez Cornejo, Carlos; Lopez Rios, Serafin [Comision Federal de Electricidad, Morelia, Michoacan (Mexico); Ley Romero, Jose R [Comision Federal de Electricidad, Guerrero Negro, Baja California Sur (Mexico)

    2000-07-01

    Comision Federal de Electricidad installed a 600 kW wind turbine in the Guerrero Negro isolated electrical grid to evaluate, under actual operation conditions, the contribution of wind energy in the generation of electricity. This paper describes the performance of the wind turbine in terms of its availability, power curve and electricity produced. The results have been satisfactory, electricity was supplied with a lesser fuel consumption than before. [Spanish] La Comision Federal de Electricidad instalo en Guerrero Negro, Baja California Sur, Mexico un aerogenerador de 600 kW para evaluar en condiciones reales de operacion la integracion de la energia del viento a sistemas electricos aislados basados en diesel. En este trabajo se describen los resultados del primer ano de operacion del aerogenerador en terminos de su disponibilidad, curva de potencia y generacion obtenida. Los resultados han sido satisfactorios, registrandose una reduccion en el consumo de combustible fosil en el sistema.

  1. Photoconductivity of Graphene in Proximity to La AlO3 /Sr TiO3 Heterostructures: Phenomenon and Photosensor Applications

    Science.gov (United States)

    Cheng, Long; Fan, Xiaodong; Wei, Laiming; Lu, Juanjuan; Liang, Haixing; Qi, Ji; Zeng, Changgan

    2016-07-01

    The proximal coupling between graphene and transition-metal-oxide heterostructures may integrate their unique features and further generate emergent states. Using the photoconductivity of graphene as an effective probe, we demonstrate the existence of a built-in polar field within the La AlO3 layer of the La AlO3 /Sr TiO3 heterostructures for both conducting and insulating La AlO3 /Sr TiO3 interfaces. Such a polar field is a prerequisite for the validity of the electronic reconstruction mechanism for the interfacial conductivity. The built-in polar field is reflected by the hole doping in the graphene in proximity to the La AlO3 /Sr TiO3 induced by pulsed deep-ultraviolet illumination regardless of the graphene's carrier type. These photoresponse characteristics also render the graphene /La AlO3 /Sr TiO3 hybrid system a convenient deep-ultraviolet sensor. Moreover, we design an efficient broad-spectrum photodetector benefiting from the large in-plane electric field in graphene across the boundary between the graphene/La AlO3 /Sr TiO3 and graphene/Sr TiO3 . Our findings may provide clues to the design of photosensors based on the hybrid structures of graphene and oxide heterostructures.

  2. Presentación [del libro "Simulación del comportamiento óseo. Aplicación al diseño de implantes"

    OpenAIRE

    Alarcón Álvarez, Enrique

    2000-01-01

    La aplicación de los fundamentos, métodos y herramientas básicas de la Mecánica del Daño Continuo a un modelo de comportamiento biológico como es la remodelación ósea supone un mutuo enriquecimiento y las posibilidades de generalización o ampliación de las disciplinas involucradas. Así surge la idea de reparación que, termodinámicamente sería imposible en un sistema mecánico puro aislado pero que aparece como conveniente y posible en un sistema simulado como mecánico pero en realidad acopl...

  3. The Utilization of ALOS PALSAR Image to Estimate Natural Forest Biomass: Case Study at Bogani Nani Wartabone National Park (Pemanfaatan Citra ALOS PALSAR dalam Menduga Biomasa Hutan Alam: Studi Kasus di Taman Nasional Bogani Nani Wartabone

    Directory of Open Access Journals (Sweden)

    Nurlita Indah Wahyuni

    2014-12-01

    Full Text Available The development of remote sensing technology makes it possible to utilize its data in many sectors including forestry. Remote sensing image has been used to map land cover and monitor deforestation. This paper presents utilization of ALOS PALSAR image to estimate and map aboveground biomass at natural forest of Bogani Nani Wartabone National Park especially SPTN II Doloduo and SPTN III Maelang. We used modeling method between biomass value from direct measurement and digital number of satellite image. There are two maps which present the distribution of biomass and carbon from ALOS PALSAR image with 50 m spatial resolution. These maps were built based on backscatter polarization of HH and HV bands. The maps indicate most research area dominated with biomass stock 0-5.000 ton/ha.

  4. Study of Sedative-Hypnotic Effects of Aloe vera L. Aqueous Extract through Behavioral Evaluations and EEG Recording in Rats.

    Science.gov (United States)

    Abdollahnejad, Fatemeh; Mosaddegh, Mahmoud; Nasoohi, Sanaz; Mirnajafi-Zadeh, Javad; Kamalinejad, Mohammad; Faizi, Mehrdad

    2016-01-01

    In this study, we investigated the sedative and hypnotic effects of the aqueous extract of Aloe vera on rats. In order to evaluate the overall hypnotic effects of the Aloe vera extract, open field and loss of righting reflex tests were primarily used. The sedative and hypnotic effects of the extract were then confirmed by detection of remarkable raise in the total sleeping time through analysis of electroencephalographic (EEG) recordings of animals. Analysis of the EEG recordings showed that there is concomitant change in Rapid Eye Movement (REM) and None Rapid Eye Movement (NREM) sleep in parallel with the prolonged total sleeping time. Results of the current research show that the extract has sedative-hypnotic effects on both functional and electrical activities of the brain.

  5. Effect of Heat Shock Treatment and Aloe Vera Coating to Chilling Injury Symptom in Tomato (Lycopersicon asculantum Mill.

    Directory of Open Access Journals (Sweden)

    Sutrisno

    2012-04-01

    Full Text Available This research was undertaken to determine the effect of length in heat shock and edible coating as pre-storage treatment to Chilling Injury (CI symptom reflected by ion leakage induced and quality properties in tomato (Lycopersicon asculantum Mill.. Heat Shock Treatment (HST was conducted at three different levels of length, which were, 20; 40 and 60 min. Edible coating was conducted using aloe vera gel. The result showed that HST and Aloe Vera Coating (AVC were more effective to reduce CI symptom at lower chilling storage. Prolong exposure to heated water may delay climacteric peak. The length of heat shock, AVC treatment and low temperature storage significantly affected the tomato quality parameter but not significantly different for each treatment except weight loss. HST for 20 min at ambient temperature was significantly different to other treatment.

  6. The addition of rosehip oil to Aloe gels improves their properties as postharvest coatings for maintaining quality in plum.

    Science.gov (United States)

    Martínez-Romero, Domingo; Zapata, Pedro J; Guillén, Fabián; Paladines, Diego; Castillo, Salvador; Valero, Daniel; Serrano, María

    2017-02-15

    The effect of Aloe vera gel (AV) and Aloe arborescens gel (AA) alone or in combination with rosehip oil (RO) at 2% on ethylene production, respiration rate, quality parameters, bioactive compounds and antioxidant activity during plum postharvest storage was studied. Coated plums showed a delay in ethylene production and respiration rate at 20°C and during cold storage and subsequent shelf life, the main effect being observed for those fruits coated with AA+RO. Quality parameters such as softening, colour and maturity index was also delayed during storage by the use of the coatings, which led to a 2-fold increase in plum storability. Accumulation of bioactive compounds was also delayed although at the end of the experiment the content of bioactive compounds was higher than those found for control fruits at the estimated shelf life. The most effective coating for maintaining plum quality and bioactive compounds was AA+RO.

  7. Propiedades físicas, estructurales y análisis de crecimiento de la Sábila (Aloe spp.)

    OpenAIRE

    Gastón Esparza Frausto; Francisco Javier Macías Rodríguez; Francisco Javier Cabral Arellano; Edgar León Esparza Ibarra; Rómulo Bañuelos Valenzuela; Ricardo David Valdez Cepeda

    2007-01-01

    Desde tiempos ancestrales se menciona el uso de la sábila (Aloe spp.) por el hom bre; sin em bargo, es a partir de la década de los 50´s cuando su cultivo co bra importancia debido a su creciente uso en la in du - stria me dic i nal, cosmetológica, alimentaria y en la elaboración de concentrados sólidos y líquidos. En este trabajo se estudiaron 10 biotipos de Aloe en los que se determinaron algunas características físicas como longitud, ancho y grosor basal de las hojas, así como algunas prop...

  8. The Online System for Lidar Data Handling and Real Time Monitoring of Lidar Operations at ALO-USU

    Science.gov (United States)

    Navarro, Luis A.; Wickwar, Vincent B.; Gamboa, Jose; Milla, Marco

    2016-06-01

    It is no longer sufficient to use lidar, such as the Rayleigh lidar at the Atmospheric Lidar Observatory (ALO) at Utah State University (USU), to observe the middle atmosphere and reduce the data to geophysical parameters. Extended operations, with inevitable equipment, data reduction, and analysis improvements, require us to keep careful track of all these changes and how they affect the scientific products. Furthermore, many of the funding agencies and the journals now require us to do, at least, some of this. We have built three interconnected data structures to organize and manage the different hardware and software setups from the Internet as well as to keep track of the products generated by these. These structures were implemented as a database, which is particularly important for groups with a large volume of information like the Rayleigh Group at ALO-USU.

  9. El derecho penal como ciencia unitaria: Una respuesta al conflicto entre el saber dogmático aislado formal y el saber disciplinar e interdisciplinar

    Directory of Open Access Journals (Sweden)

    Jaime Sandoval Fernández

    2012-01-01

    Full Text Available El derecho penal como ciencia unitaria está integrado por la dogmática, la criminología y la política criminal. La dogmática de nuestro tiempo debe ser teleológica. Está al servicio del Estado constitucional de derecho y propende reducir o contener la violencia intolerablemente irracional del poder punitivo. Este derecho penal como ciencia unitaria interactúa con otros saberes jurídicos y no jurídicos, dando lugar a relaciones disciplinares e interdisciplinares. La dogmática jurídico-penal y el derecho procesal penal en relación disciplinar deben trabajar mancomunadamente. Por supuesto, la dinámica de la dogmática deberá afinarse a los nuevos parámetros en los que transcurren los procesos penales.

  10. Aloe sterol supplementation improves skin elasticity in Japanese men with sunlight-exposed skin: a 12-week double-blind, randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Tanaka M

    2016-11-01

    Full Text Available Miyuki Tanaka,1 Yuki Yamamoto,2 Eriko Misawa,1 Kazumi Nabeshima,1 Marie Saito,1 Koji Yamauchi,1 Fumiaki Abe,1 Fukumi Furukawa2 1Functional Food Ingredients Department, Food Ingredients & Technology Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa, 2Department of Dermatology, Wakayama Medical University, Kimiidera, Wakayama, Japan Background/objective: Recently, it was confirmed that the daily oral intake of plant sterols of Aloe vera gel (Aloe sterol significantly increases the skin barrier function, moisture, and elasticity in photoprotected skin. This study aimed to investigate whether Aloe sterol intake affected skin conditions following sunlight exposure in Japanese men. Methods: We performed a 12-week, randomized, double-blind, placebo-controlled study to evaluate the effects of oral Aloe sterol supplementation on skin conditions in 48 apparently healthy men (age range: 30–59 years; average: 45 years. The subjects were instructed to expose the measurement position of the arms to the sunlight outdoors every day for 12 weeks. The skin parameters were measured at 0 (baseline, 4, 8, and 12 weeks. Results: Depending on the time for the revelation of the sunlight, the b* value and melanin index increased and the skin moisture decreased. After taking an Aloe sterol tablet daily for 12 weeks, the skin elasticity index (R2, R5, and R7 levels were significantly higher than the baseline value. There were no differences between the groups in these skin elasticity values. In the subgroup analysis of subjects aged <46 years, the change in the R5 and R7 was significantly higher in the Aloe group than in the placebo group at 8 weeks (P=0.0412 and P=0.0410, respectively. There was a difference in the quantity of sun exposure between each subject, and an additional clinical study that standardizes the amount of ultraviolet rays is warranted. No Aloe sterol intake-dependent harmful phenomenon was observed during the intake period

  11. Caracterización de hongos aislados de mapas conservados en el Archivo Nacional de la República de Cuba

    OpenAIRE

    2014-01-01

    Los microorganismos, principalmente los hongos filamentosos (mohos), generan problemas en archivos, bibliotecas y museos provocando el biodeterioro de las colecciones. Estos efectos son más acusados en países de clima tropical debido a la influenciade las altas temperaturas y humedad relativa. Los objetivos de este estudio fueron caracterizar la concentración fúngica en cinco mapas conservados en la Mapoteca del Archivo Nacional de Cuba y determinar el potencial biodeteriorante de los hongos ...

  12. 芦荟对果蝇寿命的影响%Effects of Aloe vera L on life of Drosophila

    Institute of Scientific and Technical Information of China (English)

    马红; 王红星

    2008-01-01

    以药用价值较高的库拉索芦荟(Aloe vera L.Burm.f)为材料,研究了芦荟叶的不同部分对果蝇寿命及生活周期的影响.结果表明,芦荟全叶和芦荟凝胶能明显延长果蝇的寿命、缩短生长周期.

  13. Aloe vera Leaf Anti Inflamation’s Activity Speeds Up the Healing Proccess of Oral Mucosa Ulceration

    Directory of Open Access Journals (Sweden)

    Agoeng Tjahajani

    2013-05-01

    Full Text Available Previous work showed that 25% of inner layer Aloe vera leaf extract was effective as anti-inflammatory on the oral mucous of Wistar rats. Objective: The aim of this study was to examine the lowest concentration of whole leaf Aloe vera extract that could accelerate healing process of ulceration on rats. Methods: Sixty inbreeding Spraque Dawley rats was exposed to solution of Hydrogen Peroxide 10% during three days to induced inflammation on the labial mucous inferior of rats. During the next three days, on the same area of control group were topically applied solution of Natrium Chloride 0.9%; while in the treatment group were topically applied 6.25%; 12.5%; 25% of whole leaf of Aloe vera extract for 3x5 minutes with 90 minutes intervals. On the fourth day, five rats in each group were sacrificed; and the same treatment was done on others groups. On the sixth day, five rats of each group were sacrificed; and the same treatment was done on the rest group up to seven days. On the eighth days, the rest groups were sacrificed. Microscopic slides were done. Results: Microscopic slides were analyzed under light microscope and scored. Statistical analysis with Mann-Whitney test showed significant differences between control and treatment group (p<0.05. Conclusion: Application of 6.25% whole leaf Aloe vera extract was the lowest and the most effective concentration in accelerating the healing process of oral mucous ulceration on rats.DOI: 10.14693/jdi.v18i1.56

  14. Comparative study of the efficacy of lycopene and aloe vera in the treatment of oral submucous fibrosis

    Directory of Open Access Journals (Sweden)

    Santosh Patil

    2015-01-01

    Full Text Available Background and Aim: Oral submucous fibrosis (OSMF is a high-risk premalignant condition largely seen in the Indian subcontinent. A number of studies have proven the use of antioxidants in the management of OSMF. Therefore, the aim of the present study was to compare the efficacy of two antioxidants, lycopene and aloe vera in the management of OSMF. Material and Methods: One hundred and twenty clinicopathologically diagnosed OSMF patients, were included in the study. They were divided equally into, Group A (lycopene group and Group B (aloe vera group. Group A was administered 8mg lycopene in two divided doses of 4mg daily and Group B was given5mg aloe vera gel to be applied topically thrice daily for 3 months. Different clinical parameters were evaluated at regular intervals and data was analyzed using the Student′s paired t-test and Chi-square test. P < 0.001 was considered to be statistically significant. Results: Clinical improvements in mouth opening and tongue protrusion were significant in Group A (P < 0.001. Subjective symptoms of burning sensation (P = 0.007, pain associated with the lesion (P = 0.005, and difficulty in swallowing and speech (P = 0.003 improved in both the groups, but were insignificant. There was a mild to moderate decrease in the size of the lesion. Conclusion: The present study concludes that though, there is no definitive treatment for the condition; however, lycopene can bring about significant clinical improvements in the symptoms like mouth opening and tongue protrusion when compared to aloe vera. Both the drugs appear to be promising in the treatment of OSMF.

  15. Shape- and Size-Controlled Synthesis of Silver Nanoparticles Using Aloe vera Plant Extract and Their Antimicrobial Activity

    OpenAIRE

    Logaranjan, Kaliyaperumal; Raiza, Anasdass Jaculin; Subash C B Gopinath; Chen, Yeng; Pandian, Kannaiyan

    2016-01-01

    Abstract Biogenic synthesis of silver nanoparticles (AgNP) was performed at room temperature using Aloe vera plant extract in the presence of ammoniacal silver nitrate as a metal salt precursor. The formation of AgNP was monitored by UV-visible spectroscopy at different time intervals. The shape and size of the synthesized particle were visualized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. These results were confirmed by X-ray powder diffrac...

  16. Identification of linear features at geothermal field based on Segment Tracing Algorithm (STA) of the ALOS PALSAR data

    Science.gov (United States)

    Haeruddin; Saepuloh, A.; Heriawan, M. N.; Kubo, T.

    2016-09-01

    Indonesia has about 40% of geothermal energy resources in the world. An area with the potential geothermal energy in Indonesia is Wayang Windu located at West Java Province. The comprehensive understanding about the geothermal system in this area is indispensable for continuing the development. A geothermal system generally associated with joints or fractures and served as the paths for the geothermal fluid migrating to the surface. The fluid paths are identified by the existence of surface manifestations such as fumaroles, solfatara and the presence of alteration minerals. Therefore the analyses of the liner features to geological structures are crucial for identifying geothermal potential. Fractures or joints in the form of geological structures are associated with the linear features in the satellite images. The Segment Tracing Algorithm (STA) was used for the basis to determine the linear features. In this study, we used satellite images of ALOS PALSAR in Ascending and Descending orbit modes. The linear features obtained by satellite images could be validated by field observations. Based on the application of STA to the ALOS PALSAR data, the general direction of extracted linear features were detected in WNW-ESE, NNE-SSW and NNW-SSE. The directions are consistent with the general direction of faults system in the field. The linear features extracted from ALOS PALSAR data based on STA were very useful to identify the fractured zones at geothermal field.

  17. Polysaccharides of Aloe vera induce MMP-3 and TIMP-2 gene expression during the skin wound repair of rat.

    Science.gov (United States)

    Tabandeh, Mohammad Reza; Oryan, Ahmad; Mohammadalipour, Adel

    2014-04-01

    Polysaccharides are the main macromolecules of Aloe vera gel but no data about their effect on extracellular matrix (ECM) elements are available. Here, mannose rich Aloe vera polysaccharides (AVP) with molecular weight between 50 and 250 kDa were isolated and characterized. Open cutaneous wounds on the back of 45 rats (control and treated) were daily treated with 25mg (n=15) and 50 mg (n=15) AVP for 30 days. The levels of MMP-3 and TIMP-2 gene expression were analyzed using real time PCR. The levels of n-acetyl glucosamine (NAGA), n-acetyl galactosamine (NAGLA) and collagen contents were also measured using standard biochemical methods. Faster wound closure was observed at day 15 post wounding in AVP treated animals in comparison with untreated group. At day 10 post wounding, AVP inhibited MMP-3 gene expression, while afterwards MMP-3 gene expression was upregulated. AVP enhanced TIMP-2 gene expression, collagen, NAGLA and NAGA synthesis in relation to untreated wounds. Our results suggest that AVP has positive effects on the regulation of ECM factor synthesis, which open up new perspectives for the wound repair activity of Aloe vera polysaccharide at molecular level.

  18. Haemato-immunological indices in rainbow trout (Oncorhynchus mykiss) fry fed with Aloe vera extract supplemented feed

    Institute of Scientific and Technical Information of China (English)

    Masoud Haghighi; Mostafa Sharif Rohani; Hamid Pourmoghim; Tayebeh Toliat; Meysam Samadi; Meysam Tavoli; Maryam Islami; Rahmat Yusefi

    2014-01-01

    Objective: To evaluate the effects of Aloe vera extract on the immunity responses and haematological parameters in rainbow trout (Oncorhynchus mykiss) fry to develop alternative drug to chemotherapeutics in aquaculture. Methods:Six hundred rainbow trout (Oncorhynchus mykiss) fry were randomly allocated into two treatment groups including 1) placebo-treated group (control), 2) Aloe vera extract-treated group, each of three replicates. The fishes were hand-fed once a day with diet medicated AE or placebo at the rate of 1%in feed in the first feeding for 10 weeks. At the end of the identical every two weeks 24 h after feeding, some of haematological and immunological parameters were analyzed. Results:The results showed that serum total protein, albumin and globulin, respiratory burst activity, phagocytic activity and serum lysozyme activity vary among the two treatment groups which were found to be higher in Aloe vera extract-treated group (P0.05). Conclusions: It was concluded that supplementation of AE at a rate of 1%in feed registered higher immunological responses in compared to placebo group. Therefore, supplementation of AE in fish diets enhances non-specific immune system in fish. It may use in fish diets particularly at time of outbreaks.

  19. 芦荟蜂蜜饮料的研制%Study of the Processing of Aloe and Honey Juice

    Institute of Scientific and Technical Information of China (English)

    周治德; 李桂银; 黄燕萍

    2011-01-01

    A process of Aloe and honey compound beverage was studied. The main ingredients are aloe and hon- ey. Citric acid, sugar, sodium iso-VC are used as food additives. Through a scientific study, a health benefits natural beverage was produced. In this paper, the production process and formulation was studied and a reasonable processing parameters were investigated. Orthogonal experimental results showed that the best formula was a combination of 20% of aloe vera juice, 12% of honey, 3% of sucrose, 0. 15% of citric acid. The best Stabilizers formula were 0.20% CMC with 0. 10% xanthan gum.%以库拉索芦荟和蜂蜜为主要原料,以柠檬酸、蔗糖、异Vc钠等为辅料,加工制成复合型饮料。正交实验表明,芦荟蜂蜜饮料的适宜配方为:芦荟汁20%,蜂蜜12%,白砂糖3%,柠檬酸0.15%。稳定剂为0.20羧甲基纤维素(CMC)+0.10%黄原胶。

  20. Mechanical, Thermal, and Morphological Properties of Nanocomposites Based on Polyvinyl Alcohol and Cellulose Nanofiber from Aloe vera Rind

    Directory of Open Access Journals (Sweden)

    Adel Ramezani Kakroodi

    2014-01-01

    Full Text Available This work was devoted to reinforcement of polyvinyl alcohol (PVA using cellulose nanofibers from Aloe vera rind. Nanofibers were isolated from Aloe vera rind in the form of an aqueous suspension using chemimechanical technique. Mechanical characterizations showed that incorporation of even small amounts of nanofibers (as low as 2% by weight had significant effects on both the modulus and strength of PVA. Tensile modulus and strength of PVA increased, 32 and 63%, respectively, after adding 2% of cellulose nanofiber from Aloe vera rind. Samples with higher concentrations of nanofibers also showed improved mechanical properties due to a high level of interfacial adhesion and also dispersion of fibers. The results showed that inclusion of nanofibers decreased deformability of PVA significantly. Dynamic mechanical analysis revealed that, at elevated temperatures, improvement of mechanical properties due to the presence of nanofibers was even more noticeable. Addition of nanofibers resulted in increased thermal stability of PVA in thermogravimetric analysis due to the reduction in mobility of matrix molecules. Morphological observations showed no signs of agglomeration of fibers even in composites with high cellulose nanofiber contents. Inclusion of nanofibers was shown to increase the density of composites.

  1. Antimutagenicity and Anticarcinogenic Effects of Gel and Latex Extracts of Aloe Vera Cultivated: A Comparative Study in Two Cities, Iran

    Directory of Open Access Journals (Sweden)

    Kheiri A

    2012-03-01

    Full Text Available Background: Nowadays, cancer is one of the main causes of mortality in the world and many mutagens are the cause of death in millions of patients. Due to the side effects of anticancer drugs, scientists are in search of natural drugs with fewer side effects and more therapeutic efficacy. This study aims to, firstly, investigate the antimutgenic effects of different Aloe vera gel and latex extracts on mutated Salmonella typhimurium bacterium by using Ames test and to, secondly, study the probable effects of the habitat conditions on the antimutagenic effects of the plant.Methods: After preparing different Aloe vera gel and latex extracts, the antimutagenic effects of the extracts were evaluated by Ames test. In this test, a mutated strain of S. typhimurium was grown on culture media containing a minimum of salt and glucose in the presence of a mutagen substance (NaN3. Subsequently, only those bacteria that had turned HIS+ by reverse mutation formed colonies. As different alcoholic and aqueous extracts of Aloe vera reduced reversed mutations, the difference between the means of revertant mutants per plate was calculated by one-way ANOVA using SPSS software (version 18. Results: The ethanol extracts of latex from Karaj had a maximum (91% and aqueous extract from Dezfoul had a minimum (42% percentage of inhibition. Conclusion: Maximum percentage of inhibition was observed in the extracts of the plant cultivated in Karaj reflecting the impact of environmental conditions on the construction of antioxidant compounds in plants.

  2. An investigation of age and yield of fresh fruit bunches of oil palm based on ALOS PALSAR 2

    Science.gov (United States)

    Darmawan, S.; Takeuchi, W.; Haryati, A.; M, R. Najib A.; Na'aim, M.

    2016-06-01

    The objective on this study is to investigate age and yield of FFB of oil palms based on ALOS PALSAR 2. Study areas in oil palm plantations areas of Jerantut, Pahang Malaysia. Methodology consists collecting of ALOS PALSAR 2 and tabular data on the study area, processing of ALOS PALSAR 2 including of converting digital numbers to normalize radar cross sections (NRCS), topography correction and filtering, making of regions of interest according to areas of age and yield of FFB of oil palms and making of relationship analysis between backscatter value of HH, HV and age and yield of FFB of oil palm. The results have showed relationship between HH, HV and age of oil palm which R2 of 0.63 for HH and 0.42 for HV that indicated increasing of age of oil palm as increasing of HH and HV value. Also relationship between HH, HV and yield of FFB of oil palm which R2 of 0.26 for HH and 0.15 for HV, that indicated increasing of yield of FFB as decreasing of HH and HV value.

  3. The Aloe vera phenomenon: a review of the properties and modern uses of the leaf parenchyma gel.

    Science.gov (United States)

    Grindlay, D; Reynolds, T

    1986-06-01

    The mucilaginous gel from the parenchymatous cells in the leaf pulp of Aloe vera has been used since early times for a host of curative purposes. This gel should be distinguished clearly from the bitter yellow exudate originating from the bundle sheath cells, which is used for its purgative effects. Aloe vera gel has come to play a prominent role as a contemporary folk remedy, and numerous optimistic, and in some cases extravagant, claims have been made for its medicinal properties. Modern clinical use of the gel began in the 1930s, with reports of successful treatment of X-ray and radium burns, which led to further experimental studies using laboratory animals in the following decades. The reports of these experiments and the numerous favourable case histories did not give conclusive evidence, since although positive results were usually described, much of the work suffered from poor experimental design and insufficiently large test samples. In addition some conflicting or inconsistent results were obtained. With the recent resurgence of interest in Aloe vera gel, however, new experimental work has indicated the possibility of distinct physiological effects. Chemical analysis has shown the gel to contain various carbohydrate polymers, notably either glucomannans or pectic acid, along with a range of other organic and inorganic components. Although many physiological properties of the gel have been described, there is no certain correlation between these and the identified gel components.

  4. Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study

    Directory of Open Access Journals (Sweden)

    Patrícia Fernanda Roesler Bertolini

    2012-02-01

    Full Text Available OBJECTIVES: This study evaluated in vitro the efficiency of Aloe vera and propolis dentifrice on reducing the contamination of toothbrush bristles by a standard strain of Streptococcus mutans (ATCC 25175; SM, after toothbrushing. MATERIAL AND METHODS: Fifteen sterile toothbrushes were randomly divided into 5 toothbrushing groups: I (negative control: without dentifrice; II: with fluoridated dentifrice; III: with triclosan and gantrez dentifrice; IV (positive control: without dentifrice and irrigation with 10 mL of 0.12% chlorhexidine gluconate; V: with Aloe vera and propolis dentifrice. In each group, 1 sterile bovine tooth was brushed for 1 min, where the toothbrush bristles were contaminated with 25 µL of SM. After toothbrushing, the bristles were stored in individual test tubes with 3 mL of BHI under anaerobiosis of 37°C for 48 h. Then, they were seeded with sterile swab in triplicate in the Mitis salivarius - Bacitracin culture medium. The samples were kept under anaerobiosis of 37°C for 48 h. Scores were used to count the number of colony forming units (cfu. The results were submitted to the Mann-Whitney statistical test at 5% significance level. RESULTS: There was statistically significant difference (p<0.05 for the reduction of bristle contamination comparing groups II, III, IV and V to group I. CONCLUSIONS: It may be stated that after toothbrushing, the Aloe vera and propolis dentifrice reduced the contamination of toothbrush bristles by SM, without differentiation from the other chemical agents used.

  5. Effect of Aloe Vera mouthwash on dental plaque index in patients with endotracheal tube hospitalized in intensive care unite

    Directory of Open Access Journals (Sweden)

    Nasrin Mirbastegan

    2016-06-01

    Full Text Available Introduction: Oral health is an important aspect of caring for hospitalized patients in intensive care unit (ICU. Typically, 48 hours after admission to Intensive care unit, there is a change in mouth flora and dental plaque will be created subsequently. These events make an ideal environment for microbial growth. The aim of this study was to determine the effect of Aloe Vera mouthwash on dental plaque index in patients with tracheal intubation at ICU. Material and Methods: This randomized double blind trail was performed on 79 patients with Tracheal intubation at ICU. Data were gathered using purposive sampling. Participant divided in to the two groups. The experimental group received Aloe Vera mouth wash and control group received Normal Saline mouth wash. The study period was four days. O’leary Plaque index used for collecting data. Kolmogorov-Smirnov, Chi square, paired t-test and independent ttest were used for analyzing data by SPSS 20. Result: The experimental and control groups had similar characteristics except gender. The experimental dental plaque index (56.58±11.91 and Control dental plaque index (56.04±10.46 were similar. The dental plaque index had a significant decrease in experimental group (57.51±9.08 comparing to the control group (62.46±9.46. Conclusion: Aloe Vera mouth wash along with tooth brushing have a significant effect on reducing dental plaque index.

  6. Genetic toxicity assessment of aloe capsule%芦荟软胶囊的遗传毒性评价

    Institute of Scientific and Technical Information of China (English)

    贾玉巧; 白国胜; 王宇梅; 田俊丽

    2011-01-01

    目的:探讨某品牌芦荟软胶囊的遗传毒性.方法:使用小鼠经口急性毒性试验、小鼠骨髓嗜多染红细胞微核试验、鼠伤寒沙门氏菌回复突变(Ames)试验、和小鼠精子畸形试验对芦荟软胶囊的遗传毒性进行评价.结果:芦荟软胶囊对小鼠的经口急性毒性LD50大于20 ml/kgBW(最大灌胃容量),属实际无毒物质.微核试验、Ames试验和精子畸形试验结果均为阴性.结论:在本次试验条件下,该品牌芦荟软胶囊未显示有遗传毒性作用.%Objective: To study the genetic toxicity of aloe capsule. Methods: A cute toxicity test of mice, micronucleus test of bone marrow PCE cell in mice, Ames test,sperm shape abnormality test of mice. Results: The aloe capsule was a substance with no toxicity according to the acute toxicity. The results of genetic toxicity test were all negative, including micronucleus test, Ames test and sperm shape abnormality test. Conclusion: The aloe capsule has no genetic toxicity in this test.

  7. del investigador en psicología en México

    Directory of Open Access Journals (Sweden)

    Rocío Angélica González Romo

    2007-01-01

    Full Text Available Partiendo de la necesidad de fomentar la investigación y de los retos en la formación de investigadores, se exploró el proceso creativo de investigadores en psicología con base las dimensiones de persona, proceso, producto y contexto. Participaron 88 investigadores de 26 entidades federativas de México, quienes respondieron un cuestionario diseñado ad hoc. Los datos se procesaron mediante análisis de conglomerados. En el presente trabajo se reportan los resultados de las dimensiones y subdimensiones de creatividad analizadas, que revelan tres conglomerados con diferencias significativas en los perfiles del proceso creativo: investigadores en formación, investigadores aislados e investigadores consolidados. Finalmente, se discuten las implicaciones de estos hallazgos para la formación de investigadores en psicología.

  8. Responses of broilers to Aloe vera bioactives as feed additive: The effect of different forms and levels of bioactives on performances of broilers

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    A.P Sinurat

    2002-06-01

    Full Text Available Feed additives are commonly used in poultry feed as growth promotors or to improve feed efficiency. Previous results showed that Aloe vera bioactives could improve feed efficiency in broilers. Therefore, a further study was designed in order to obtain optimum doses and application methods of bioactives for broiler chickens. Aloe vera was prepared in different forms (fresh gel, dry gel, fresh whole leaf or dry whole leaf. The aloe was supplemented into the feed with concentrations of 0.25; 0.5 and 1 g/kg (equal to dry gel. Standard diets with or without antibiotics were also included as control. The diets were fed to broilers from day old to 5 weeks and the performances were observed. Results showed that the aloe-bioactives did not significantly (P>0.05 affect final body weight of broilers as compared with the control. Supplementation of 0.25 g/kg fresh gel, 0.25 and 1.0 g/kg dry gel significantly improved feed convertion by 4.7; 4.8 and 8.2%, respectively as compared with the control. This improvement was a result of reduction in feed intake or dry matter intake without reducing the weight gain. However, supplementation of whole aloe leafs could not improve feed convertion in boilers. It is concluded that the bioactives of Aloe vera could be used as feed supplement to improve feed efficiency in broilers with no deleterious effect on weight gain, carcass yield, abdominal fat levels and internal organs. The effective concentrations of aloe gell as a feed supplement based on dry matter convertion were from 0.25 g/kg fresh gel, 0.25 and 1.0 g/kg dry gel.

  9. 芦荟固体饮料加工工艺研究%Research on Processing Technology of Aloe Solid Drink

    Institute of Scientific and Technical Information of China (English)

    李勇; 刘全德; 宋慧; 陈尚龙; 王进; 包欲乾

    2011-01-01

    The production process of aloe solid drink was made by experiments and the important points were discussed.The smaller the aloe leaf thickness was,the shorter the drying time costed.The average product yield of aloe juice reached 40.44% and the total sugar of crude polysaccharide from aloe was 68.12% by experiments.The color remains the same,when the sodium erythorbate in aloe juice was 0.100 mg/mL.The optimal allocation was found by orthogonal experiment,as follows: aloe juice 91.872%,sucrose 4%,maltodextrin 2%,trehalose 2%,citric acid 0.125%.After dried the product productivity of aloe solid drink was 11.56%.%试验确定了芦荟固体饮料的生产工艺流程和操作要点。试验表明芦荟叶厚度越小,所需干燥时间越短,芦荟粗提物中多糖含量为68.12%。芦荟汁平均得率为40.44%,当原汁中异VC钠的浓度达到0.100 mg/mL时,褐变将不再有明显的变化。通过正交试验确定了芦荟饮料最佳调配方案为:91.872%芦荟原汁,4%白砂糖,2%麦芽糊精,2%海藻糖,0.125%浸柠檬酸。干燥后的芦荟固体饮料产品出率为11.56%。

  10. AISLAMIENTO DE Bacillus SOLUBILIZADORES DE FOSFATOS ASOCIADOS AL CULTIVO DEL ARROZ

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    Berto Tejera-Hern\\u00E1ndez

    2013-01-01

    Full Text Available El objetivo de este trabajo fue determinar la capacidad de bacterias del género Bacillus pro- venientes del cultivo del arroz (Oryza sativa L. en solubilizar fosfatos. Los aislamientos se obtuvieron utilizando el modelo Microcosmos de los cultivares Iacuba 30 y Perla, en el año 2008 en Bauta, La Habana, Cuba. Hubo 58 aislados, tanto rizosféricos como endófitos de la raíz y de la parte aérea, que presentaban características distintivas del género Bacillus, como respuesta positiva a la tinción de Gram, forma bacilar y formación de endospora. Se realizó la detección cualitativa en cuanto a la capacidad de solubilización de fosfatos. Solo 19 aislamientos mostraron solubilización en medio sólido NBRIP y en algunos casos cambiaron la coloración del medio de azul a amarillo, lo que indica la producción de ácidos. Se seleccionaron los positivos para la cuantificación en medio líquido. Las cepas mostraron un comportamiento diferente cuando se midió el fosfato solubilizado a distintos tiempos, variando el máximo de solubilización.

  11. Ética del trabajo y ética de la acumulación en Locke

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    Dante A. Baranzelli

    2007-11-01

    Full Text Available En este artículo propongo una interpretación del concepto lockeano de propiedad privada, desarrollado principalmente en el quinto capítulo del Segundo tratado sobre el gobierno civil. Por un lado, señalo que Locke es un portavoz temprano de la ética del trabajo -concepto extraído de Zygmunt Bauman-, para la cual la actividad apropiadora es una prerrogativa y a su vez un deber del hombre; por otro, subrayo la importancia del dinero como pieza clave de su incipiente capitalismo y sostengo que el trabajo se resignifica en la economía monetaria, según la entiende Locke, y pasa a estar subordinado a lo que denomino una ética de la acumulación.

  12. Comparative evaluation of Bacillus licheniformis 5A5 and Aloe variegata milk-clotting enzymes

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    S. A. Ahmed

    2012-03-01

    Full Text Available The properties of a milk clotting enzyme (MCE produced by bacteria (Bacillus licheniformis 5A5 were investigated and compared to those of rennet extracted from a plant (Aloe variegata. Production of MCE by B. licheniformis 5A5 was better in static than in shaken cultures. Maximum activity (98.3 and 160.3 U/ml of clotting was obtained at 75ºC and 80ºC with bacterial and plant rennet, respectively. In the absence of substrate, the clotting activity of Aloe MCE was found to be less sensitive to heat inactivation up to 80ºC for 75 min, retaining 63.8% of its activity, while bacterial MCE was completely inhibited. CaCl2 stimulated milk clotting activity (MCA up to 2% and 1.5% for bacterial and plant enzymes. NaCl inhibited MCA for both enzymes, even at low concentration (1%. Plant MCE was more sensitive to NaCl at 3% concentration it retained 30.2% of its activity, whereas bacterial MCE retained 64.1%. Increasing skim milk concentration caused a significant increase in MCA up to 6% for both enzymes. Mn2+ stimulated the activity of bacterial and plant enzymes to 158.6 and 177.9%, respectively. EDTA and PMSF increased the activity of plant MCE by 34.4 and 41.1%, respectively, which is higher than those for the bacterial MCE (19.1 and 20.9%. Some natural materials activated MCE, the highest activation of bacterial MCE (128.1% was obtained in the presence of Fenugreek (with acid extraction. However Lupine Giza 1 (with neutral extraction gave the highest activation of plant MCE (137.9%. All extracts from Neem plant increased MCA at range from 105.6% to 136.4%. Plant MCE exhibited much better stability when stored at room temperature (25-30ºC for 30 days, retaining 51.2% of its activity. Bacterial MCE was highly stabile when stored under freezing (-18ºC, retaining 100% of its activity after 30 days. Moreover, bacterial MCE was highly tolerant to repeated freezing and thawing without loss of activity for 8 months.

  13. Antibacterial Effect of Aloe Vera Gel against Oral Pathogens: An In-vitro Study

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    Jain, Supreet; Rathod, Nirav; Sur, Jaideep; Laheji, Afshan; Gupta, Naveen; Agrawal, Priyanka; Prasad, Swati

    2016-01-01

    Introduction Natural herbal remedies have shown promising anti-microbial property and fewer side effects compared to synthetic anti-microbial therapy. Aloe Vera is a medicinal plant used for management of various infections since ancient times as it has anti-inflammatory, anti-microbial, and immune-boosting properties. Aim The aim of the present study was to determine the anti-microbial and inhibitory activities of various concentration of Aloe Vera Gel (AVG) against oral pathogenic bacteria. Materials and Methods Subgingival calculus and aspiration of periapical abscess and periodontal abscess was done in 20 patients and the sample transferred to thioglycolate broth, which was incubated in Mutans Sanguis agar, blood agar and cultured in anaerobic gas chamber. The colonies formed were identified further by gram staining methods and biochemical fermentation tests (IMViC). Each isolated colony of identified bacteria were cultured separately in Muller-Hilton broth and incubated at 37°C for 24 hours. Anti-microbial activity of the AVG was tested by the disc diffusion method and minimum inhibitory concentration was determined by broth micro-dilution method. Result Various staining and biochemical tests confirmed that the sample contained Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans), Clostridium bacilli (C. bacilli), Streptcoccus mutans (S. mutans) and Staphlococcus aureus (Staph. aureus). AVG showed anti-bacterial property at 100% and 50% concentration (‘t’ value = 7.504, p-value <0.001). At lower concentration there was no effect against the bacteria. At 100% AVG concentration, zone of inhibition measured was 6.9mm in A. actinomycetemcomitans, 6.3mm in C. bacilli, 6.8mm in S. mutans and 6.6mm in Staph. aureus. The standard drugs were also used to compare anti-bacterial property of AVG. Result showed that higher concentration (100%, 50%) of AVG has comparable zone of inhibition with Ofloxacin (5mcg) and Ciprofloxacin (30mcg). Conclusion AVG at

  14. Antifibrotic effect of aloe vera in viral infection-induced hepatic periportal fibrosis

    Institute of Scientific and Technical Information of China (English)

    Sahar K Hegazy; Mohamed El-Bedewy; Akira Yagi

    2012-01-01

    AIM:To investigate the anti-oxidative and anti-fibrotic effects of aloe vera in patients with liver fibrosis.METHODS:Aloe vera high molecular weight fractions (AHM) were processed by patented hyper-dry system in combination of freeze-dry technique with microwave and far infrared-ray radiation.Fifteen healthy volunteers as the control group and 40 patients were included.The patients were randomly subdivided into two equal groups:the conventional group was treated with placebo (starch),and AHM group was treated with 0.15 gm/d AHM,both for 12 consecutive weeks.The patients were investigated before and after treatment.Serum activity of aspartate aminotransferase (AST),alanine aminotransferase (ALT),alkaline phosphatase (ALP),hyaluronic acid (HA),transforming growth factor-β (TGF-β) and matrixmetalloproteinase-2 (MMP-2) were determined.The reduced glutathione (GSH) and malondialdehyde (MDA) levels in liver were assayed and the expression of hepatic α-smooth muscle actin (α-SMA)was identified by immunohistochemistry.RESULTS:At the start of the study,the hematoxylin and eosin staining revealed fibro-proliferated bile ductules,thick fibrous septa and dense inflammatory cellular infiltration in the patients before treatment.The use of AHM for 12 wk significantly ameliorated the fibrosis,inhibited the inflammation,and resulted in minimal infiltration and minimal fibrosis compared to the conventional group.The enzyme activities of the liver (ALT,AST and ALP) were attenuated after treatment in both groups,and the decrease in the AHM group was more significant as compared with the conventional group.Similar to the AST,the MDA levels were significantly higher before treatment,and were attenuated after treatment in both groups.In contrast,the hepatic glutathione content in the patients were decreased significantly in the AHM group compared to the controls.The serum levels of the fibrosis markers (HA,TGF-β and MMP-2) were also reduced significantly after treatment

  15. Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats

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    Atiba A

    2015-10-01

    Full Text Available Ayman Atiba,1 Tamer Wasfy,2 Walied Abdo,3 Ahmed Ghoneim,2 Tarek Kamal,4 Mustafa Shukry5 1Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 2Department of Ophthalmology, Faculty of Medicine, Tanta University, 3Department of Pathology, 4Department of Biochemistry, 5Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt Purpose: To investigate the efficacy of topical applied aloe vera (AV and to facilitate the repair of the standardized alkaline corneal ulcer in normal and diabetic rats.Materials and methods: The corneal alkali-burn injury model was established unilaterally in Wistar rats by filter paper saturated with 0.01 M NaOH contacting the eyes for 45 seconds. Rats were divided into four groups: normal control (NC, normal AV (NAV, diabetic control (DC, and diabetic AV (DAV. NAV and DAV groups were treated with AV gel eye drops four times daily, and NC and DC groups were treated with normal saline for 3 days. Corneal epithelial wound closure and degree of edema were recorded using slit lamp and optical coherence tomography at 0, 24, 48, and 72 hours postwounding. Histological examination was conducted to evaluate the degree of inflammation and the healing effect.Results: Corneal epithelial wound healing was better in the NAV group than in the NC group, and it was significantly higher in the DAV group than in the DC group (P<0.05. In comparison to the DC group, DAV treated with AV demonstrated a marked reduction in edema at 48 and 72 hours. Histologically, corneal re-epithelialization was complete and higher in DAV group than that in DC group; moreover, the inflammatory cells were increased in DC group than DAV group (P<0.05.Conclusion: This study demonstrated the efficacy of AV for enhanced corneal re-epithelialization, as well as reduced inflammatory response after alkali burn in rats; therefore, it could be useful as a

  16. Making SAR Data Accessible - ASF's ALOS PALSAR Radiometric Terrain Correction Project

    Science.gov (United States)

    Meyer, F. J.; Arko, S. A.; Gens, R.

    2015-12-01

    While SAR data have proven valuable for a wide range of geophysical research questions, so far, largely only the SAR-educated science communities have been able to fully exploit the information content of internationally available SAR archives. The main issues that have been preventing a more widespread utilization of SAR are related to (1) the diversity and complexity of SAR data formats, (2) the complexity of the processing flows needed to extract geophysical information from SAR, (3) the lack of standardization and automation of these processing flows, and (4) the often ignored geocoding procedures, leaving the data in image coordinate space. In order to improve upon this situation, ASF's radiometric terrain-correction (RTC) project is generating uniformly formatted and easily accessible value-added products from the ASF Distributed Active Archive Center's (DAAC) five-year archive of JAXA's ALOS PALSAR sensor. Specifically, the project applies geometric and radiometric corrections to SAR data to allow for an easy and direct combination of obliquely acquired SAR data with remote sensing imagery acquired in nadir observation geometries. Finally, the value-added data is provided to the user in the broadly accepted Geotiff format, in order to support the easy integration of SAR data into GIS environments. The goal of ASF's RTC project is to make SAR data more accessible and more attractive to the broader SAR applications community, especially to those users that currently have limited SAR expertise. Production of RTC products commenced October 2014 and will conclude late in 2015. As of July 2015, processing of 71% of ASF's ALOS PALSAR archive was completed. Adding to the utility of this dataset are recent changes to the data access policy that allow the full-resolution RTC products to be provided to the public, without restriction. In this paper we will introduce the processing flow that was developed for the RTC project and summarize the calibration and validation

  17. Detecting and Mapping Invasive Phragmites australis in the coastal Great Lakes with ALOS PALSAR imagery

    Science.gov (United States)

    Bourgeau-chavez, L. L.; Scarbrough, K.; Jenkins, L. K.; Riordan, K.; Powell, R. B.; Brooks, C.; Kowalski, K.; Carlson Mazur, M.; Huberty, B.

    2011-12-01

    Phragmites australis is a non-native invasive plant that can form dense monocultures, causing negative impacts on coastal Great Lakes wetlands by reducing ecosystem services including habitat and therefore, biological diversity. Through Great Lakes Restoration Initiative funding, ALOS PALSAR imagery is being used to map the invasive plant as it occurs in monoculture stands of the U.S. coastal Great Lakes wetlands. These invasive Phragmites maps are being used as part of a USGS Great Lakes Science Center (GLSC) and US Fish and Wildlife Service (USFWS) National Wetlands Inventory (NWI) program to identify major environmental drivers of invasive Phragmites distribution, to assess areas vulnerable to new invasion, and to provide this information to regional stakeholders through a decision support tool. The invasive Phragmites map is the first U.S. basin-wide map to be produced on the distribution of this species. Methods include maximum likelihood classification of multi-season ALOS PALSAR HH and HV polarization data. PALSAR is an L-band (23 cm wavelength) imaging radar sensor which is sensitive to differences in plant biomass and inundation patterns, allowing for the extraction of these tall (up to 15 m), high-density, high-biomass Phragmites wetland stands. To improve discrimination of Phragmites australis, the three date (spring, summer, fall) dataset is being used, which takes advantage of phenological changes in vegetation and inundation patterns over the seasons. Field collections of training and randomly selected validation data were conducted in spring summer and fall of 2010-11 to aid in the mapping and for accuracy assessment. The minimum mapping unit is 1/2 acre and thus all field sites were sampled at 1/2 acre units. All map products and field validation data will be complete by December 2011. Maps are being completed on a Lake basin basis. The first final map product was delivered for Lake Erie coastal wetlands to 10 km inland, with an overall map accuracy

  18. Chemical and physical properties of aloe vera (Aloe barbadensis Miller gel stored after high hydrostatic pressure processing Propriedades químicas e físicas do gel de aloe vera (Aloe barbadensis Miller armazenado após processamento sob alta pressão hidrostática

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    Karina Di Scala

    2013-03-01

    Full Text Available The aim of this study was to evaluate the influence of high hydrostatic pressure (150, 250, 350, 450, and 550 MPa, applied for 5 minutes, on antioxidant capacity, total phenolic content, color, firmness, rehydration ratio, and water holding capacity of aloe vera gel stored for 60 days at 4 °C. The analyzed properties of the pressurized gel showed significant changes after the storage period. The highest value of total phenolic content was found at 550 MPa. However, a decrease in the antioxidant capacity was observed for all pressurized gel samples when compared to the control sample (p O objetivo deste estudo foi avaliar o efeito da aplicação de altas pressões hidrostáticas (150, 250, 350, 450 e 550 MPa aplicadas durante 5 minutos sobre a atividade antioxidante, concentração de polifenóis totais, cor, firmeza, taxa de reidratação e capacidade de retenção de água do gel de aloe vera armazenado durante 60 dias a 4 °C. As propriedades analisadas do gel pressurizado mostraram mudanças significativas depois de armazenadas. Para pressões de 550 Mpa, registrou-se o maior aumento de polifenóis totais. No entanto, observou-se uma diminuição da capacidade antioxidante em todas as amostras, em comparação com a amostra não tratada (p < 0,05. As alterações de cor foram menores para pressões entre 150 e 250 MPa. A aplicação de altas pressões hidrostáticas diminuiu a firmeza do gel, registrando-se o menor valor para 150 MPa (p < 0,05. Por outro lado, o tratamento sem pressão mostrou uma maior perda de firmeza, indicando que o tratamento por altas pressões conserva esta propriedade. A aplicação de altas pressões evidenciou modificações da matriz do alimento, avaliadas como taxa de reidratação e capacidade de retenção de água.

  19. 芦荟糯米保健醋的研制%Study on aloe glutinous rice vinegar

    Institute of Scientific and Technical Information of China (English)

    陈军; 赵晓燕; 李亚玲

    2012-01-01

    The aloe glutinous rice vinegar was prepared with aloe juice, glutinous rice, dry yeast and acetic acid bacteria. The ferment technique was studied by mono -factor experiment and ortbogonal experiment. The optimal combination was yeast inoculation amount 1.0% ( V/V), ratio of rice to aloe juice 3: 2( g/mL), added water amount was 1.5 times of aloe and glutinous rice, temperature 28 ℃. The optimal acetic acid fermentation was fermentation at 30 ℃ for 96 h with an acetic acid bacteria inoculation amount of 4% (V/V) and 10% alcohol (V/V). The concentration of the health vinegar with aloe and glutinous rice was 6.48 g/100 mL with unique flavor and taste pure.%以芦荟原汁、糯米、酿酒干酵母、醋酸菌为原料,通过单因素实验和正交试验,探索了芦荟糯米醋的发酵工艺。通过正交试验确定了最佳组合为酵母活化液接种量为1.0%(V/V)、糯米与芦荟汁混合比为3:2(g/mL),加水量为芦荟糯米混合量的1.5倍,发酵温度为28℃。芦荟糯米醋的最佳醋酸发酵条件为发酵温度30℃、酒精度10%(V/V)、接种量4%(V/V)、发酵时间96h,可获得6.48g/100mL芦荟糯米保健醋。酿制的芦荟糯米醋香味独特,品位纯正。

  20. Del pergamino al bit. La influencia del soporte en el contenido

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    Nistal, Ana

    2009-06-01

    Full Text Available The printed book has been one of the most revolutionary developments in European history; the coming of the book was possible tanks to the development of the printing industry. The evolution of the new technology around the book industry caused profound changes affecting authors, producers and readers. New medium favoured new ways to create contents. Digital technology is pushing another revolution that threatens to change the world of the book, not only in terms of physical media, but also the concept of isolated book, closed by cardboard covers, with delimited beginning and end. The big change is not the transition from printed characters to bits; the change will be the transition from isolated books to connected books, which can be accessed trough multiples ways (or “links”, open books against to the closed books of the Gutenberg age.El libro moderno de papel ha sido, sin duda, uno de los instrumentos más revolucionarios en la historia europea; su aparición fue posible gracias a las nuevas tecnologías de impresión y edición en papel. El cambio que supuso el desarrollo industrial de los libros cambió totalmente la concepción de los creadores, distribuidores y lectores. Los nuevos y modernos soportes propiciaron nuevas formas para crear contenidos antes imposibles. La tecnología digital está propiciando otra gran revolución que amenaza con cambiar el mundo del libro, no sólo en cuanto al soporte físico, sino a su concepto como libro aislado, delimitado por unas tapas de cartón, con un principio y un fin concretos. El gran cambio no es el paso de letras impresas a bits, sino el paso de libros aislados a libros “conectados”, a los que se puede acceder mediante infinitas fórmulas (o “conexiones” en oposición a los libros cerrados de la era Gutenberg.

  1. Genotipos de aislados de campo de Brucella abortus de distintas regiones geográficas de Chile Genotypes of Brucella abortus field isolates from different geographical regions of Chile

    Directory of Open Access Journals (Sweden)

    M Mancilla

    2008-01-01

    Full Text Available La brucelosis bovina es una enfermedad zoonótica, endémica de alto impacto económico. La identificación genética de las cepas prevalentes de Brucella abortus, el patógeno, es clave para establecer estrategias epidemiológicas de control de la enfermedad. La secuencia de inserción IS711 ha sido utilizada como un marcador genético para diferenciar entre especies de Brucella, miembros de una misma especie y dentro de un mismo biovar. Hemos analizado los perfiles de IS711-RFLP de 46 aislados de B. abortus, recolectados durante el periodo 1997-2005, provenientes de 16 áreas geográficas diferentes de Chile. Todos los aislados fueron previamente identificados como B. abortus biovar 1, utilizando las técnicas convencionales. De estos, el 87% compartieron el mismo perfil de IS711-RFLP, mientras que el 8,7% correspondió al patrón de la cepa vacuna RB51. En este trabajo se informa el hallazgo de dos cepas indistinguibles por PCR AMOS con perfiles nuevos de IS711-RFLP, no reportados previamente.Bovine brucellosis is an endemic, zoonotic disease of high economic impact. The genetic identification of the prevalent Brucella abortus strains, the pathogen, is key to pursue further epidemiological strategies for disease control. The insertion sequence IS711 has been used as genetic marker to differentiate among Brucella species, members of the same specie and within the same biovar. We have analyzed the IS711-RFLP pattern for 46 B. abortus isolates, collected during the period of 1997-2005 from 16 different geographical areas of Chile. All isolates were previously identified by conventional techniques as B. abortus biovar 1. Of these, 87% sharedthesame IS711 DNA profile, while an 8.7 % corresponded to the pattern of RB51 vaccine strain. We report the finding of two new strains, not differentiated by AMOS PCR, which showed unreported patterns of IS711-RFLP.

  2. Influence of drinking water containing Aloe vera (Aloe barbadensis Miller) gel on growth performance, intestinal microflora, and humoral immune responses of broilers

    Science.gov (United States)

    Shokraneh, Meisam; Ghalamkari, Gholamreza; Toghyani, Majid; Landy, Nasir

    2016-01-01

    Aim: The risk of bacteria resistance to specific antibiotics possibly by continuous subtherapeutical administration of antibiotic growth promoters (AGPs) in poultry feed led to a ban on the use of AGP in poultry production. As a result of this ban, alternative substances for poultry growth promotion and disease prevention are being investigated, among which phytogenic and herbal products have received increased attention as natural additives because they have been accepted by consumers as natural additives. The effect of water supplementation of Aloe vera (AV) as an AGP substitute on performance, intestinal microflora, and immune responses of broilers. Materials and Methods: The five experimental treatments were allocated to four replicates. The following treatments were applied (1) a basal broiler diet (C) and normal drinking water, (2) 0.5% AV gel in drinking water, (3) 0.75% AV gel in drinking water, (4) 1% AV gel in drinking water, and (5) diet C supplemented with flavophospholipol at 4.5 mg/kg and drinking normal water. Vaccines against influenza disease and sheep red blood cell (SRBC) were administrated to immunological stimuli. The populations of Lactobacilli spp. and coliforms were enumerated in ileum. Results: Body weight of broilers supplemented with different levels of AV increased compared with control group (p<0.05). Birds supplemented with antibiotic had the best feed-to-gain ratio (F:G) in different periods. Supplementation of 0.5% and 0.75% AV improved F: G entire experimental period compared with control group (p<0.05). Coliform bacteria were reduced in broilers supplemented with different levels of AV or antibiotic (p<0.05). The Lactobacilli spp. population in birds supplemented with 0.75%, 1% AV or antibiotic significantly was higher than other groups (p<0.05). Supplementation with 1% AV led to greater antibody titers against SRBC compared with other groups (p<0.05). Conclusion: These findings demonstrated a possibility of supplementing broiler

  3. Influence of drinking water containing Aloe vera (Aloe barbadensis Miller gel on growth performance, intestinal microflora, and humoral immune responses of broilers

    Directory of Open Access Journals (Sweden)

    Meisam Shokraneh

    2016-11-01

    Full Text Available Aim: The risk of bacteria resistance to specific antibiotics possibly by continuous subtherapeutical administration of antibiotic growth promoters (AGPs in poultry feed led to a ban on the use of AGP in poultry production. As a result of this ban, alternative substances for poultry growth promotion and disease prevention are being investigated, among which phytogenic and herbal products have received increased attention as natural additives because they have been accepted by consumers as natural additives. The effect of water supplementation of Aloe vera (AV as an AGP substitute on performance, intestinal microflora, and immune responses of broilers. Materials and Methods: The five experimental treatments were allocated to four replicates. The following treatments were applied (1 a basal broiler diet (C and normal drinking water, (2 0.5% AV gel in drinking water, (3 0.75% AV gel in drinking water, (4 1% AV gel in drinking water, and (5 diet C supplemented with flavophospholipol at 4.5 mg/kg and drinking normal water. Vaccines against influenza disease and sheep red blood cell (SRBC were administrated to immunological stimuli. The populations of Lactobacilli spp. and coliforms were enumerated in ileum. Results: Body weight of broilers supplemented with different levels of AV increased compared with control group (p<0.05. Birds supplemented with antibiotic had the best feed-to-gain ratio (F:G in different periods. Supplementation of 0.5% and 0.75% AV improved F:G entire experimental period compared with control group (p<0.05. Coliform bacteria were reduced in broilers supplemented with different levels of AV or antibiotic (p<0.05. The Lactobacilli spp. population in birds supplemented with 0.75%, 1% AV or antibiotic significantly was higher than other groups (p<0.05. Supplementation with 1% AV led to greater antibody titers against SRBC compared with other groups (p<0.05. Conclusion: These findings demonstrated a possibility of supplementing

  4. CLONACIÓN Y FILOGENIA MOLECULAR DE UN SEGMENTO DEL GEN CODANTE DE LA ACTINA DE MYRCIARIA DUBIA “CAMU-CAMU”: UN CANDIDATO PARA GEN DE REFERENCIA

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    Juan Carlos Castro Gómez

    2012-12-01

    Full Text Available Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del gen. En base a la secuencia obtenida se diseñaron cebadores específicos para PCR en tiempo real. Los resultados muestran que se ha aislado, clonado y secuenciado un segmento del gen codante de actina de M. dubia y detectado su expresión en hojas, pulpa y cáscara de M. dubia. Así, con el soporte de herramientas bioinformáticas y uso de técnicas de biología molecular hemos aislado, clonado y secuenciado un segmento del gen codante de la actina de M. dubia. Asimismo, los análisis realizados muestran que el gen se expresa y presenta niveles similares de expresión en hojas, pulpa y cáscara de M. dubia. Sin embargo, es necesario realizar más experimentos a fin de verificar su estabilidad de expresión.

  5. Protección jurídica del ambiente en la contratación mercantil. Estudio práctico del caso colombiano

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    Rafael E. Wilches Durán

    2015-01-01

    Full Text Available Este artículo tiene como objetivo efectuar un estudio de índole práctica sobre la protección jurídica del ambiente en la contratación mercantil en Colombia, recurriendo para ello a un análisis de la contratación verde en el país, utili zando como criterio de evaluación el principio de precaución. Las conclusio nes obtenidas tienen que ver con los pocos y aislados avances sobre la materia en el país, con la necesidad de que el derecho incorpore el asunto dentro de su discurso, y con que, no solo desde el plano teórico sino desde el práctico, la contratación mercantil y la protección del ambiente manejan discursos distintos, cuya relación puede ser abordada a la luz del planteamiento de la colisión de discursos que ofrece la teoría del derecho reflexivo.

  6. Procesado acuoso de soja con tecnología enzimática: extracción de aceite y producción de aislados

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    Domínguez, H.

    1995-02-01

    Full Text Available An enzymatic treatment was incorporated before the alkaline extraction during the aqueous processing of soya beans for oil and protein. The degradation on the plant cell wall structure by the enzymatic action, enhances the oil extractability by 10% of the potential extractable oil. No significant effect on the protein extractability was detected, although this effect was probably masked by the alkaline extraction. After the aqueous extraction, 30% of active enzyme was eliminated in the whey. This can be recovered and reutilized in the next stage.

    Se ha incorporado una etapa de tratamiento enzimático previa a la extracción acuosa en condiciones alcalinas durante el procesado para obtención de aceite y proteína de soja. La degradación de la estructura de la pared vegetal, causada por el ataque enzimático, aumenta la extractabilidad del aceite en un 10% respecto al aceite total extraíble. No se apreció efecto sobre el rendimiento de proteína debido posiblemente a que la posterior extracción a pH 9 inhibe los efectos de la acción enzimática. Tras la realización del proceso de extracción acuoso, se elimina en el suero un 30% de enzima activa, que puede ser reutilizada en el proceso.

  7. Combination of Aloe vera and xenograft induction on decreasing of NF-kb of tooth extraction socket preservation in Cavia cobaya

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    Utari Kresnoadi

    2014-03-01

    Full Text Available Background: Tooth extraction can naturally cause inflammation triggering osteoclast proliferation and alveolar bone resorption. Preservation of the tooth extraction sockets is needed for patients in order to reduce alveolar bone resorption risks. Aloe vera is known to have anthraquinones components, namely Aloin, Aloe emedin, and barbaloin, considered as anti-inflammation. Therefore, to overcome the inflammation, the role of NF-kb is very significant to decrease nuclear factor kappa b (NF-kb. As a result, inflammation risks will be decreased. Purpose: The study was aimed to determine the induction effect of combination of Aloe vera and XCB into tooth extraction sockets to reduce inflammation by reducing NF-kb expression, osteoclasts and osteoblasts. Methods: Forty-eight Cavia cobaya were divided into eight groups, each group consisted of six animals. The mandibular incisors of those Cavia cobaya were extracted and induced with either PEG, XCB, Aloe vera, or the combination of Aloe vera + XCB. Those animals were sacrificed on day 7 and day 30 after the extraction. Then immunohistochemical and histopathology examinations were conducted to observe NF-kb expression, osteoblasts and osteoclasts. Results: It was known that in group induced with the combination of Aloe vera and xenograft concelous bovine, the growth of osteoblasts was high, while NF-kb expression and osteoclasts reduced. Conclusion: It can be concluded that the induction of the combination of Aloe vera and XCB into the tooth extraction sockets can reduce NF-kb expression and osteoclast, as a result, alveolar bone resorption risks decrease, and osteoblast increase.Latar belakang: Trauma mekanis akibat pencabutan gigi asli menyebabkan keradangan. Keradangan memicu proliferasi osteoklas sehingga menyebabkan resorpsi tulang alveolararis. Pada pembuatan gigi tiruan, resorpsi tulang alveolar yang terjadi, sangat tidak diinginkan, sebab resorpsi tulang alveolar mengurangi keberhasilan

  8. Accumulation of Heavy Metals and Role of EDTA in Aloe vera L.%芦荟对土壤中重金属的吸收及EDTA影响

    Institute of Scientific and Technical Information of China (English)

    王畅; 张涛; 仇荣亮; 郭鹏然

    2012-01-01

    Distribution and effects of EDTA on accumulation of heavy metals (HM) in aloe (Aloe vera L.) were investigated by pot experiments. Results showed that HM concentrations in aloe increased with the increase of HM concentrations in soil, especially in leaves for Cd, Cu and Zn and in stems and roots for Pb. Addition of Pb to soil enhanced the concentrations of Cd and Cu in aloe, which indicated the synergistic effect of Pb and Cd, Cu. With the increase of EDTA concentration of 0-4 mmol/kg, HM concentrations in aloe increased. Translocation of HM increased due to effects of EDTA, and sequence of translocation factor is Zn, Cd, Cu and Pb. Chelating effects promoted the accumulation of HM in aloe. Accumulation of Fe in leaves of aloe was inhibited by HM, and Ca accumulation in leaves stems and roots of aloe were all inhibited by HM.%采用芦荟(Aloe vera L.)盆栽实验,考察了土壤中重金属在芦荟中的分布及施加EDTA对重金属在芦荟中积累影响.由实验结果,芦荟中重金属随土壤中重金属含量的增加而增加,Cd、Cu和Zn的增加量主要积累在叶部,而Pb的增加量主要积累在茎和根部.土壤中Pb的加入促进了芦荟对Cd和Cu的吸收,表明Pb与Cd、Cu在芦荟吸收时具有协同效应.随着土壤中EDTA用量的增加(0~4 mmol/kg),芦荟对重金属的吸收积累增加,其中Cu和Pb的增加较大.在EDTA作用下,芦荟叶部对重金属的迁移增大,其迁移系数Zn>Cd>Cu>Pb.EDTA螯合作用促使芦荟对重金属的吸收.芦荟叶部重金属对Fe的积累有明显抑制作用,而芦荟各部位中重金属对Ca的积累皆有明显抑制作用.

  9. Evaluation of the Antioxidative, Antibacterial, and Anti-Inflammatory Effects of the Aloe Fermentation Supernatant Containing Lactobacillus plantarum HM218749.1

    Science.gov (United States)

    Deng, Kan; Jiang, Chunling; Fu, Mingui; Guo, Chunlan; Wang, Xiaolei; Wang, Xin; Meng, Fanjing; Yang, Shaoguo; Deng, Keyu

    2016-01-01

    Little work is done to develop Aloe vera (AV) using probiotics. To explore the potential benefits, the antioxidant effects and the antibacterial effects on foodborne pathogens of Aloe fermentation supernatant were evaluated in vitro. Our results indicated that the Aloe fermentation supernatant fermented by Lactobacillus plantarum HM218749.1 had very strong scavenging capacities of the DPPH (86%), O2•− (85%), •OH (76%), and Fe2+ chelation (82%) and reducing powers (242.5 mg/L), and the inhibition zones for Salmonella typhimurium, Salmonella enteritidis, Shigella flexneri, Escherichia coli, Listeria monocytogenes, S. dysenteriae 301, Staphylococcus aureus Cowan1, and Propionibacterium acnes were 16, 15, 19, 20, 21, 20, and 27 mm. Moreover, the low concentration of Aloe fermentation supernatant had significantly reduced the production of IL-1β, TNF-α, and IL-6 in both mRNA and protein levels (P < 0.01). Therefore, the Aloe fermentation supernatant can be used as functional beverage or cosmetic ingredients to guard human intestinal health, delaying senescence, and prevent chronic diseases. PMID:27493450

  10. A comparative study of the effects of topical application of Aloe vera, thyroid hormone and silver sulfadiazine on skin wounds in Wistar rats.

    Science.gov (United States)

    Tarameshloo, Mahsa; Norouzian, Mohsen; Zarein-Dolab, Saeed; Dadpay, Masoomeh; Gazor, Roohollah

    2012-03-01

    Many research studies report the healing effects of Aloe Vera, thyroid hormone cream and silver sulfadiazine. However, the effects of these therapeutic agents are not well understood and have not been compared in one study. This study aimed at investigating the effects of topical application of an Aloe vera gel, a thyroid hormone cream and a silver sulfadiazine cream on the healing of skin wounds surgically induced in Wistar rats for determining the treatment of choice. In a randomized controlled trial, twelve male rats, aged 120 days and with a mean weight of 250 to 300 g, were divided randomly into 5 groups based on drug treatments: Aloe vera gel (AV), thyroid hormone cream (TC), silver sulfadiazine 1% (S), vehicle (V) and control. To evaluate the efficacy of each treatment technique, a biomechanical approach was used to assess tensile stress after 14 days of treatment. Tensile stress was significantly improved in the Aloe vera gel group as compared with the other four groups (P≤0.05). While the other treatment options resulted in better healing than the control group, this difference was not significant. We conclude that Aloe vera topical application accelerated the healing process more than thyroid hormone, silver sulfadiazine and vehicle in surgically induced incisions in rats.

  11. Evaluation of the Antioxidative, Antibacterial, and Anti-Inflammatory Effects of the Aloe Fermentation Supernatant Containing Lactobacillus plantarum HM218749.1

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    Meixiu Jiang

    2016-01-01

    Full Text Available Little work is done to develop Aloe vera (AV using probiotics. To explore the potential benefits, the antioxidant effects and the antibacterial effects on foodborne pathogens of Aloe fermentation supernatant were evaluated in vitro. Our results indicated that the Aloe fermentation supernatant fermented by Lactobacillus plantarum HM218749.1 had very strong scavenging capacities of the DPPH (86%, O2•- (85%, OH• (76%, and Fe2+ chelation (82% and reducing powers (242.5 mg/L, and the inhibition zones for Salmonella typhimurium, Salmonella enteritidis, Shigella flexneri, Escherichia coli, Listeria monocytogenes, S. dysenteriae 301, Staphylococcus aureus Cowan1, and Propionibacterium acnes were 16, 15, 19, 20, 21, 20, and 27 mm. Moreover, the low concentration of Aloe fermentation supernatant had significantly reduced the production of IL-1β, TNF-α, and IL-6 in both mRNA and protein levels (P<0.01. Therefore, the Aloe fermentation supernatant can be used as functional beverage or cosmetic ingredients to guard human intestinal health, delaying senescence, and prevent chronic diseases.

  12. Efficacy of Aloe Vera Supplementation on Prediabetes and Early Non-Treated Diabetic Patients: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

    Science.gov (United States)

    Zhang, Yiyi; Liu, Wen; Liu, Dan; Zhao, Tieyun; Tian, Haoming

    2016-06-23

    The aim of this study was to evaluate evidence for the efficacy of aloe vera on managing prediabetes and early non-treated diabetes mellitus. We performed a systematic search of PubMed, Embase, and Cochrane Central Register of Controlled Trials until 28 January 2016. A total of five randomized controlled trials (RCTs) involving 415 participants were included. Compared with the controls, aloe vera supplementation significantly reduced the concentrations of fasting blood glucose (FBG) (p = 0.02; weighed mean difference [WMD]: -30.05 mg/dL; 95% confidence interval [CI]: -54.87 to -5.23 mg/dL), glycosylated hemoglobin A1c (HbA1c) (p Aloe vera was superior to placebo in increasing serum high density lipoprotein-cholesterol (HDL-C) levels (p = 0.04). Only one adverse event was reported. The evidence from RCTs showed that aloe vera might effectively reduce the levels of FBG, HbA1c, triglyceride, TC and LDL-C, and increase the levels of HDL-C on prediabetes and early non-treated diabetic patients. Limited evidence exists about the safety of aloe vera. Given the small number and poor quality of RCTs included in the meta-analysis, these results are inconclusive. A large-scale, well-designed RCT is needed to further address this issue.

  13. Efficacy of Aloe Vera Supplementation on Prediabetes and Early Non-Treated Diabetic Patients: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

    Science.gov (United States)

    Zhang, Yiyi; Liu, Wen; Liu, Dan; Zhao, Tieyun; Tian, Haoming

    2016-01-01

    The aim of this study was to evaluate evidence for the efficacy of aloe vera on managing prediabetes and early non-treated diabetes mellitus. We performed a systematic search of PubMed, Embase, and Cochrane Central Register of Controlled Trials until 28 January 2016. A total of five randomized controlled trials (RCTs) involving 415 participants were included. Compared with the controls, aloe vera supplementation significantly reduced the concentrations of fasting blood glucose (FBG) (p = 0.02; weighed mean difference [WMD]: −30.05 mg/dL; 95% confidence interval [CI]: −54.87 to −5.23 mg/dL), glycosylated hemoglobin A1c (HbA1c) (p Aloe vera was superior to placebo in increasing serum high density lipoprotein-cholesterol (HDL-C) levels (p = 0.04). Only one adverse event was reported. The evidence from RCTs showed that aloe vera might effectively reduce the levels of FBG, HbA1c, triglyceride, TC and LDL-C, and increase the levels of HDL-C on prediabetes and early non-treated diabetic patients. Limited evidence exists about the safety of aloe vera. Given the small number and poor quality of RCTs included in the meta-analysis, these results are inconclusive. A large-scale, well-designed RCT is needed to further address this issue. PMID:27347994

  14. EVALUATION OF FREEZE DRIED ALOE VERA AND NOPAL CACTUS FOR POSSIBLE HEALTH TREATMENTS BY COMPARISON OF ANTIOXIDANT PROPERTIES AND FREE RADICAL INHIBITION.

    Science.gov (United States)

    Rodriguez, Kenneth R; Jones, Anthony E; Belmont, Barbara

    2014-01-01

    The goal of this project was to characterize the antioxidant powers of lyophilized Aloe Vera (Aloe barbadensis) and Nopal Cactus (Opuntia ficus-indica) by quantifying the phenolics content and radical scavenging abilities of preparations derived from these plants. Extracts of these lyophylized succulents were assayed for phenolic compounds by the Folin Ciocalteau method and compared for free radical scavenging capability by the DPPH method. We found that even though the Aloe lyophilizate extract contained more phenolic content, the Nopal lyophilizate exhibited better free radical scavenging ability. Aloe Vera extract contained 0.278 g/L of phenolic content and exhibited 11.1% free radical inhibition, with a free radical scavenging rate constant of 0.177±0.015 min(-1). Nopal Cactus extract contained 0.174 g/L of phenolic content and exhibited 13.2% free radical inhibition, with a free radical scavenging rate constant of 0.155±0.009 min(-1). These results showed Nopal to have greater antioxidant potency than Aloe.

  15. Metagenomics study of endophytic bacteria in Aloe vera using next-generation technology

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    Mushafau Adewale Akinsanya

    2015-12-01

    Full Text Available Next generation sequencing (NGS enables rapid analysis of the composition and diversity of microbial communities in several habitats. We applied the high throughput techniques of NGS to the metagenomics study of endophytic bacteria in Aloe vera plant, by assessing its PCR amplicon of 16S rDNA sequences (V3–V4 regions with the Illumina metagenomics technique used to generate a total of 5,199,102 reads from the samples. The analyses revealed Proteobacteria, Firmicutes, Actinobacteria and Bacteriodetes as the predominant genera. The roots have the largest composition with 23% not present in other tissues. The stems have more of the genus—Pseudomonas and the unclassified Pseudomonadaceae. The α-diversity analysis indicated the richness and inverse Simpson diversity index of the bacterial endophyte communities for the leaf, root and stem tissues to be 2.221, 6.603 and 1.491 respectively. In a similar study on culturable endophytic bacteria in the same A. vera plants (unpublished work, the dominance of Pseudomonas and Bacillus genera was similar, with equal proportion of four species each in root, stem and leaf tissues. It is evident that NGS technology captured effectively the metagenomics of microbiota in plant tissues and this can improve our understanding of the microbial–plant host interactions.

  16. Phytochemical Contents and Antioxidant Capacities of Two Aloe greatheadii var. davyana Extracts

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    Du Toit Loots

    2008-09-01

    Full Text Available Aloe greatheadii var. davyana (Asphodelaceae is used among rural South African communities to treat arthritis, skin cancer, burns, eczema, psoriasis, digestive problems, high blood pressure and diabetes, despite very little supporting scientific evidence. Due to increased interest by both the scientific community and industry regarding the medicinal uses of this plant species, we identified, quantified and compared the phytochemical contents and antioxidant capacities of two extracts of A. greatheadii; a leaf gel extract (LGE and a 95 % aqueous ethanol leaf gel extract (ELGE, using various modified extraction procedures, GC-MS and spectrophotometry. Apart from extensively characterizing this medicinal plant with regards to its organic acid, polyphenols/phenolic acid, alcohol, aldehyde, ketone, alkane, pyrimidine, indole, alkaloid, phytosterol, fatty acid and dicarboxylic acid contents and antioxidant capacities, we describe a modified extraction procedure for the purpose of general phytochemical characterization, and compare this to a 95 % aqueous ethanol extraction technique. From the results it is clear that A. greatheadii contains a variety of compounds with confirmed antioxidant capacity and other putative health benefits (such as blood glucose, cholesterol and cortisol lowering properties relating to the prevention or treatment of diabetes, cardiovascular disease, cancer and hypertension. The results also indicate that separate ethyl acetate/diethyl ether and hexane extractions of the LGE, better serve for general phytochemical characterization purposes, and 95 % aqueous ethanol extraction for concentrating selective groups of health related compounds, hence justifying its use for biological in vivo efficacy studies.

  17. Crude Aloe vera Gel Shows Antioxidant Propensities and Inhibits Pancreatic Lipase and Glucose Movement In Vitro

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    Urmeela Taukoorah

    2016-01-01

    Full Text Available Aloe vera gel (AVG is traditionally used in the management of diabetes, obesity, and infectious diseases. The present study aimed to investigate the inhibitory potential of AVG against α-amylase, α-glucosidase, and pancreatic lipase activity in vitro. Enzyme kinetic studies using Michaelis-Menten (Km and Lineweaver-Burk equations were used to establish the type of inhibition. The antioxidant capacity of AVG was evaluated for its ferric reducing power, 2-diphenyl-2-picrylhydrazyl hydrate scavenging ability, nitric oxide scavenging power, and xanthine oxidase inhibitory activity. The glucose entrapment ability, antimicrobial activity, and total phenolic, flavonoid, tannin, and anthocyanin content were also determined. AVG showed a significantly higher percentage inhibition (85.56±0.91 of pancreatic lipase compared to Orlistat. AVG was found to increase the Michaelis-Menten constant and decreased the maximal velocity (Vmax of lipase, indicating mixed inhibition. AVG considerably inhibits glucose movement across dialysis tubes and was comparable to Arabic gum. AVG was ineffective against the tested microorganisms. Total phenolic and flavonoid contents were 66.06±1.14 (GAE/mg and 60.95±0.97 (RE/mg, respectively. AVG also showed interesting antioxidant properties. The biological activity observed in this study tends to validate some of the traditional claims of AVG as a functional food.

  18. In vivo macrophage activation in chickens with Acemannan, a complex carbohydrate extracted from Aloe vera.

    Science.gov (United States)

    Djeraba, A; Quere, P

    2000-05-01

    Acemannan (ACM 1), a beta-(1,4) -acetylated mannan isolated from Aloe vera, can be used as an effective adjuvant in vaccination against some avian viral diseases. Our results demonstrate a quick and lasting in vivo priming effect of ACM 1 on macrophage response after intramuscular inoculation in chickens (500 microg per 2-month-old bird). In response to IFN-gamma in vitro, monocytes from ACM 1-treated chickens exhibited a strong enhancement of NO production from 3 to 9 days p.i., but a weaker effect on MHC II cell surface antigen expression on day 3 p.i. A stimulating effect of ACM 1 treatment was also observed on spontaneous and inducible NO production for splenocytes only on day 3 p.i. By that time, splenocytes exhibited a strong higher capacity to proliferate in response to the T cell-mitogen PHA. At the same time, the in vivo capacity to produce NO, measured by the (NO(-)(2)+NO(-)(3)) serum level after intravenous LPS injection, increased greatly from 3 to 9 days p.i. In conclusion, ACM 1 was able efficiently and durably to increase the activation capacity of macrophages from the systemic immune compartment