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Sample records for aggregatibacter actinomycetemcomitans y4

  1. Al-2 of Aggregatibacter actinomycetemcomitans inhibits Candida albicans biofilm formation

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    Bachtiar, Endang W.; Bachtiar, Boy M.; Jarosz, Lucja M.; Amir, Lisa R.; Sunarto, Hari; Ganin, Hadas; Meijler, Michael M.; Krom, Bastiaan P.

    2014-01-01

    Aggregatibacter actinomycetemcomitans, a Gram-negative bacterium, and Candida albicans, a polymorphic fungus, are both commensals of the oral cavity but both are opportunistic pathogens that can cause oral diseases. A. actinomycetemcomitans produces a quorum-sensing molecule called autoinducer-2 (Al

  2. Differential transcription of virulence genes in Aggregatibacter actinomycetemcomitans serotypes

    OpenAIRE

    Marcia Pinto Alves MAYER; Umeda, Josely Emiko; Priscila Larcher LONGO; Simionato, Maria Regina Lorenzetti

    2013-01-01

    Background: Aggregatibacter actinomycetemcomitans serotypes are clearly associated with periodontitis or health, which suggests distinct strategies for survival within the host.Objective: We investigated the transcription profile of virulence-associated genes in A. actinomycetemcomitans serotype b (JP2 and SUNY 465) strains associated with disease and serotype a (ATCC 29523) strain associated with health. Design: Bacteria were co-cultured with immortalized gingival epithelial cells (OBA-9). T...

  3. Antibiotic susceptibility of Aggregatibacter actinomycetemcomitans JP2 in a biofilm

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    Oettinger-Barak, Orit; Dashper, Stuart G.; Catmull, Deanne V.; Geoffrey G. Adams; Michael N. Sela; Machtei, Eli E.; Reynolds, Eric C.

    2013-01-01

    Background: Localized aggressive periodontitis (LAgP) is an inflammatory disease associated with specific bacteria, particularly Aggregatibacter actinomycetemcomitans, which can result in early tooth loss. The bacteria grow as a biofilm known as subgingival plaque. Treatment includes mechanical debridement of the biofilm, often associated with empirical antibiotic treatment.Objective: The aims of this study were to test in vitro the sensitivity of A. actinomycetemcomitans JP2 during planktoni...

  4. AI-2 of Aggregatibacter actinomycetemcomitans Inhibits Candida albicans Biofilm Formation

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    Endang W. Bachtiar

    2014-07-01

    Full Text Available Aggregatibacter actinomycetemcomitans, a Gram-negative bacterium, and Candida albicans, a polymorphic fungus, are both commensals of the oral cavity but both are opportunistic pathogens that can cause oral diseases. A. actinomycetemcomitans produces a quorum-sensing molecule called autoinducer-2 (AI-2, synthesized by LuxS, that plays an important role in expression of virulence factors, in intra- but also in interspecies communication. The aim of this study was to investigate the role of AI-2 based signaling in the interactions between C. albicans and A. actinomycetemcomitans. A. actinomycetemcomitans adhered to C. albicans and inhibited biofilm formation by means of a molecule that was secreted during growth. C. albicans biofilm formation increased significantly when co-cultured with A. actinomycetemcomitans luxS, lacking AI-2 production. Addition of wild-type-derived spent medium or synthetic AI-2 to spent medium of the luxS strain, restored inhibition of C. albicans biofilm formation to wild-type levels. Addition of synthetic AI-2 significantly inhibited hypha formation of C. albicans possibly explaining the inhibition of biofilm formation. AI-2 of A. actinomycetemcomitans is synthesized by LuxS, accumulates during growth and inhibits C. albicans hypha- and biofilm formation. Identifying the molecular mechanisms underlying the interaction between bacteria and fungi may provide important insight into the balance within complex oral microbial communities.

  5. Antibiotic susceptibility of Aggregatibacter actinomycetemcomitans JP2 in a biofilm

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    Orit Oettinger-Barak

    2013-05-01

    Full Text Available Background: Localized aggressive periodontitis (LAgP is an inflammatory disease associated with specific bacteria, particularly Aggregatibacter actinomycetemcomitans, which can result in early tooth loss. The bacteria grow as a biofilm known as subgingival plaque. Treatment includes mechanical debridement of the biofilm, often associated with empirical antibiotic treatment. Objective: The aims of this study were to test in vitro the sensitivity of A. actinomycetemcomitans JP2 during planktonic and biofilm growth to doxycycline and to the combination of metronidazole and amoxicillin, which are two antibiotic protocols commonly used in clinical practice. Design: Two in vitro biofilm models were used to test the effects of the antibiotics: a static 96-well plate assay was used to investigate the effect of these antibiotics on biofilm formation whilst a flow chamber model was used to examine the effect on established biofilms. Results: Of the antibiotics tested in this model system, doxycycline was most efficacious with a minimal inhibitory concentration (MIC against planktonic cells of 0.21 mg/L and minimal biofilm inhibitory concentration (MBIC of 2.10 mg/L. The most commonly prescribed antibiotic regimen, amoxicillin + metronidazole, was much less effective against both planktonic and biofilm cells with an MIC and MBIC of 12.0 mg/L and 20.2 mg/L, respectively. A single treatment of the clinically achievable concentration of 10 mg/L doxycycline to sparse A. actinomycetemcomitans biofilms in the flow chamber model resulted in significant decreases in biofilm thickness, biovolume, and cell viability. Dense A. actinomycetemcomitans biofilms were significantly more resistant to doxycycline treatment. Low concentrations of antibiotics enhanced biofilm formation. Conclusion: A. actinomycetemcomitans JP2 homotypic biofilms were more susceptible in vitro to doxycycline than amoxicillin + metronidazole.

  6. Paparan zat besi pada ekspresi protein spesifik extracellular polymeric substance biofilm Aggregatibacter actinomycetemcomitans

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    Marchella Hendrayanti W

    2014-06-01

    Full Text Available Background: The study of biofilms bacteria could be an alternative of preventive treatment in reducing prevalence of aggressive periodontitis in the community, because biofilm protects the bacteria from environmental conditions, including the attack of immune system and antimicrobial. Aggregatibacter actinomycetemcomitans is a major cause of bacterial aggressive periodontitis. Purpose: This study aims to examine the iron exposure to specific protein expression of extracellular polymeric substance (EPS of Aggregatibacter actinomycetemcomitans biofilm. Methods: Protein containing EPS biofilm was isolated from cultures of A.actinomycetemcomitans. The protein was processed through several procedures: electrophoresis , electroelution , immunization of rabbits , serum isolation , and purification of antibodies. After the Western blotting procedure the antibody was used. Protein containing EPS biofilms exposed to iron, then once again isolated from cultures of A. actinomycetemcomitans. The electrophoresis and Western blotting were done on the isolated protein. Results: The result showed that the the expression of specific proteins in EPS biofilm decreased in response to iron exposure. Conclusions: Iron exposure could influenced the specific protein expression in EPS biofilm of Aggregatibacter actinomycetemcomitans.Latar belakang: Penelitian terhadap bakteri biofilm dapat menjadi alternatif perawatan preventif dalam menurunkan prevalensi periodontitis agresif di masyarakat, karena biofilm melindungi bakteri terhadap kondisi lingkungan, termasuk serangan sistem imun dan antimikroba. Aggregatibacter actinomycetemcomitans merupakan bakteri penyebab utama periodontitis agresif. Tujuan: Studi ini bertujuan meneliti paparan zat besi terhadap ekspresi protein spesifik extracellular polymeric substance (EPS Aggregatibacter actinomycetemcomitans. Metode: Protein yang mengandung EPS biofilm diisolasi dari kultur A. actinomycetemcomitans. Protein yang diisolasi

  7. tfoX (sxy)-dependent transformation of Aggregatibacter (Actinobacillus) actinomycetemcomitans

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    Bhattacharjee, Mrinal K.; Fine, Daniel H.; Figurski, David H.

    2007-01-01

    tfoX (sxy) is a regulatory gene needed to turn on competence genes. Aggregatibacter (Actinobacillus) actinomycetemcomitans has a tfoX gene that is important for transformation. We cloned this gene on an IncQ plasmid downstream of the inducible tac promoter. When this plasmid was resident in cells of A. actinomycetemcomitans and tfoX was induced, the cells became competent for transformation. Several strains of A. actinomycetemcomitans, including different serotypes, as well as rough (adherent...

  8. Prevalence and distribution of Aggregatibacter actinomycetemcomitans and its cdtB gene in subgingival plaque of Chinese periodontitis patients

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    Wang, Xiaoqian; Li, Lu; Yang, Mifang; Geng,Ying; Chen, Huiping; XU, Yan(Department of Music,Guangxi Normal University); Sun, Ying

    2014-01-01

    Background Aggregatibacter actinomycetemcomitans (A.actinomycetemcomitans) is an important periodontal pathogen that can participate in periodontitis and other non-oral infections. The cytolethal distending toxin (Cdt) is among the virulence factors produced by this bacterium. This study was to elucidate the distribution of A.actinomycetemcomitans and the prevalence of its cdtB gene in Chinese subjects. Methods A total of 255 subgingival samples were obtained from 30 subjects. Samples were co...

  9. Occurrence of Aggregatibacter actinomycetemcomitans in Brazilians with chronic periodontitis

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    José Roberto Cortelli; Caio Vinícius Gonçalves Roman-Torres; Davi Romeiro Aquino; Gilson Cesar Nobre Franco; Fernando Oliveira Costa; Sheila Cavalca Cortelli

    2010-01-01

    Studies have suggested that A. actinomycetemcomitans is involved in the aetiology of aggressive periodontitis as well as in chronic periodontitis. This study was aimed at elucidating the occurrence of A. actinomycetemcomitans in a Brazilian population with chronic periodontitis. A total of 555 (mean age 33.04 ± 12.45) individuals, living in two large areas of the São Paulo State, namely "Baixada Santista" and "Vale do Paraíba", and diagnosed with mild [180 (mean age 29.59 ± 10.94)], moderate ...

  10. Antimicrobial proteins of Snail mucus (Achatina fulica against Streptococcus mutans and Aggregatibacter actinomycetemcomitans

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    Herluinus Mafranenda DN

    2014-03-01

    Full Text Available Background: Achasin and mytimacin-AF are proteins of snail mucus (Achatina fulica which have antimicrobial activity. Snail mucus is suspected to have other proteins which have antimicrobial activity against Streptococcus mutans and Aggregatibacter actinomycetemcomitans the oral pathologic bacteria. Purpose: The study were aimed to characterize the proteins of snail mucus (Achatina fulica that have antimicrobial activities to Streptococcus mutans and Actinobacillus actinomycetemcomitans, and to compared the antimicrobial effect of achasin and mytimacin-AF. Methods: The sample of study was the mucus of snails which were taken from Yogyakarta Province. The isolation and characterization of protein were conducted by using SDS-PAGE method, electroelution, and dialysis. Nano drop test was conducted to determine protein concentration. The sensitivity test was conducted by using dilution test, and followed by spectrophotometry and paper disc diffusion tests. Results: The study showed that proteins successfully characterized from snail mucus (Achatina fulica were proteins with molecular weights of 83.67 kDa (achasin, 50.81 kDa, 15 kDa, 11.45 kDa (full amino acid sequence of mytimacin-AF and 9.7 kDa (mytimacin-AF. Based on the dilution test, Achasin had better antimicrobial activities against Streptococcus mutans, while mytimacin-AF had better antimicrobial activities against Aggregatibacter actinomycetemcomitans. But the paper disc diffusion test result showed that Achasin had antimicrobial activities against Streptococcus mutans and Aggregatibacter actinomycetemcomitans, while mytimacin-AF had no antimicrobial activities. Conclusion: The proteins with molecular weights of 50.81 kDa, 15 kDa, 11.45 kDa were considered as new antimicrobial proteins isolated from snail mucus. Achasin, had better antimicrobial activities against Streptococcus mutans, while mytimacin-AF had better antimicrobial activities against Aggregatibacter actinomycetemcomitans

  11. ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF SYZYGIUM AROMATICUM (L.) MERR.PERRY (CLOVES) AGAINST CLINICAL ISOLATES OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS.

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    Ramanath Karicheri; Beena Antony

    2015-01-01

    Aim: Aggregatibacter actinomycetemcomitans implicated in the etiology of aggressive, chronic periodontitis and other systemic infections. The aim of th is study was to investigate the antibacterial efficacy of essential oil of a traditional medicinal plant, Syzygium aromaticum ( L .) Merr.perry (cloves) against the clinical isolates of A.actinomycetemcomitans obtained from the patients with orodental infections. Methodology: Essential oil of clove flower buds was distilled by Neo-Clevenger’s ...

  12. Trimeric form of intracellular ATP synthase subunit β of Aggregatibacter actinomycetemcomitans binds human interleukin-1β.

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    Annamari Paino

    Full Text Available Bacterial biofilms resist host defenses and antibiotics partly because of their decreased metabolism. Some bacteria use proinflammatory cytokines, such as interleukin (IL-1β, as cues to promote biofilm formation and to alter virulence. Although one potential bacterial IL-1β receptor has been identified, current knowledge of the bacterial IL-1β sensing mechanism is limited. In chronic biofilm infection, periodontitis, Aggregatibacter actinomycetemcomitans requires tight adherence (tad-locus to form biofilms, and tissue destroying active lesions contain more IL-1β than inactive ones. The effect of IL-1β on the metabolic activity of A. actinomycetemcomitans biofilm was tested using alamarBlue™. The binding of IL-1β to A. actinomycetemcomitans cells was investigated using transmission electron microscopy and flow cytometry. To identify the proteins which interacted with IL-1β, different protein fractions from A. actinomycetemcomitans were run in native-PAGE and blotted using biotinylated IL-1β and avidin-HRP, and identified using mass spectroscopy. We show that although IL-1β slightly increases the biofilm formation of A. actinomycetemcomitans, it reduces the metabolic activity of the biofilm. A similar reduction was observed with all tad-locus mutants except the secretin mutant, although all tested mutant strains as well as wild type strains bound IL-1β. Our results suggest that IL-1β might be transported into the A. actinomycetemcomitans cells, and the trimeric form of intracellular ATP synthase subunit β interacted with IL-1β, possibly explaining the decreased metabolic activity. Because ATP synthase is highly conserved, it might universally enhance biofilm resistance to host defense by binding IL-1β during inflammation.

  13. Human Serum-Specific Activation of Alternative Sigma Factors, the Stress Responders in Aggregatibacter actinomycetemcomitans

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    Tang-Siegel, Gaoyan; Bumgarner, Roger; Ruiz, Teresa; Kittichotirat, Weerayuth; Chen, Weizhen; Chen, Casey

    2016-01-01

    Aggregatibacter actinomycetemcomitans, a known pathogen causing periodontal disease and infective endocarditis, is a survivor in the periodontal pocket and blood stream; both environments contain serum as a nutrient source. To screen for unknown virulence factors associated with this microorganism, A. actinomycetemcomitans was grown in serum-based media to simulate its in vivo environment. Different strains of A. actinomycetemcomitans showed distinct growth phenotypes only in the presence of human serum, and they were grouped into high- and low-responder groups. High-responders comprised mainly serotype c strains, and showed an unusual growth phenomenon, featuring a second, rapid increase in turbidity after 9-h incubation that reached a final optical density 2- to 7-fold higher than low-responders. Upon further investigation, the second increase in turbidity was not caused by cell multiplication, but by cell death. Whole transcriptomic analysis via RNA-seq identified 35 genes that were up-regulated by human serum, but not horse serum, in high-responders but not in low-responders, including prominently an alternative sigma factor rpoE (σE). A lacZ reporter construct driven by the 132-bp rpoE promoter sequence of A. actinomycetemcomitans responded dramatically to human serum within 90 min of incubation only when the construct was carried by a high responder strain. The rpoE promoter is 100% identical among high- and low-responder strains. Proteomic investigation showed potential interactions between human serum protein, e.g. apolipoprotein A1 (ApoA1) and A. actinomycetemcomitans. The data clearly indicated a different activation process for rpoE in high- versus low-responder strains. This differential human serum-specific activation of rpoE, a putative extra-cytoplasmic stress responder and global regulator, suggests distinct in vivo adaptations among different strains of A. actinomycetemcomitans. PMID:27490177

  14. Leukotoxicity of Aggregatibacter actinomycetemcomitans in generalized aggressive periodontitis in Brazilians and their family members

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    Virginia Regia Souza Silveira

    2013-09-01

    Full Text Available OBJECTIVE: The purpose of this study was to examine the leukotoxin promoter types of Aggregatibacter actinomycetemcomitans clones in subjects with generalized aggressive periodontitis (GAgP and in their family members (FM. MATERIAL AND METHODS: Thirty-five patients with GAgP (33.9±7.1 years, 33 of their FM (22.8±11.4 years, and 41 patients with chronic periodontitis (CP (44.1±9.4 years were clinically analyzed using the plaque index, gingival index, probing depth (PD, and clinical attachment level (CAL. Subgingival biofilm samples were collected from four interproximal periodontal sites (>PD and >CAL of each patient. The presence of A. actinomycetemcomitans and its leukotoxic clone was confirmed by polymerase chain reaction (PCR. RESULTS: A. actinomycetemcomitans was observed in 23 (51.1% GAgP patients and 16 (30.1% CP patients. Thirty-seven (94.8% patients showed minimally leukotoxic strains and 2 (5.1% showed highly leukotoxic strains. In the FM group, 10 (30.3% had aggressive periodontitis (AgP, 12 (36.3% had CP, 11 (33.3% were periodontally healthy or had gingivitis, and 12.2% were A. actinomycetemcomitans positive. Greater full mouth PD and CAL were observed in GAgP patients positive for the bacteria than those negative for it (p<;0.05, and the presence of A. actinomycetemcomitans positively correlated with GAgP (Odds ratio, 3.1; confidence interval, 1.4-7.0; p=0.009. CONCLUSIONS: The presence of A. actinomycetemcomitans was associated with the clinical condition of GAgP, with most patients exhibiting a generalized form of the disease and minimally leukotoxic clones. Most of the relatives of GAgP patients presented either CP or AgP.

  15. Profound Effects of Aggregatibacter actinomycetemcomitans Leukotoxin Mutation on Adherence Properties Are Clarified in in vitro Experiments.

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    Velusamy, Senthil Kumar; Sampathkumar, Vandana; Godboley, Dipti; Fine, Daniel H

    2016-01-01

    Leukotoxin (Ltx) is a prominent virulence factor produced by Aggregatibacter actinomycetemcomitans, an oral microorganism highly associated with aggressive periodontitis. Ltx compromises host responsiveness by altering the viability of neutrophils, lymphocytes, and macrophages. Previously, we developed a Rhesus (Rh) monkey colonization model designed to determine the effect of virulence gene mutations on colonization of A. actinomycetemcomitans. Unexpectedly, an A. actinomycetemcomitans leukotoxin (ltxA) mutant (RhAa-VS2) failed to colonize in the Rh model. No previous literature suggested that Ltx was associated with A. actinomycetemcomitans binding to tooth surfaces. These results led us to explore the broad effects of the ltxA mutation in vitro. Results indicated that LtxA activity was completely abolished in RhAa-VS2 strain, while complementation significantly (Phard tissue binding in vitro, which helps explain the previous in vivo failure of a ltxA knockout to colonize the Rh oral cavity. These results suggest that; 1) one specific gene knockout (in this case ltxA) could affect other seemingly unrelated genes (such as rcpA, rcpB tadA etc), and 2) some caution should be used when interpreting the effect attributed to targeted gene mutations when seen in a competitive in vivo environment.

  16. Genomic Stability of Aggregatibacter actinomycetemcomitans during Persistent Oral Infection in Human.

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    Ruoxing Sun

    Full Text Available The genome of periodontal pathogen Aggregatibacter actinomycetemcomitans exhibits substantial variations in gene content among unrelated strains primarily due to the presence or absence of genomic islands. This study examined the genomic stability of A. actinomycetemcomitans during its persistent infection in the same host. Four pairs of A. actinomycetemcomitans strains, each pair isolated from an individual over time (0-10 years, were examined for their gains/losses of genes by whole genome sequencing, comparative genomic hybridization by microarray and PCR analysis. Possible effects due to genomic changes were further assessed by comparative transcriptome analysis using microarrays. The results showed that each pair of strains was clonally identical based on phylogenetic analysis of 150 core genes. A novel 24.1-Kb plasmid found in strain S23A was apparently lost in the sibling strain I23C. A 353-bp inversion affecting two essential genes of the serotype-specific gene cluster was found in the serotype antigen-nonexpressing strain I23C, while the same gene cluster was intact in the serotype-expressing sibling strain S23A. A 2,293-bp deletion affecting a gene encoding oxaloacetate decarboxylase and its neighbor region was found in strain SCC2302 but not in the sibling strain AAS4a. However, no evidence of gains or losses of genomic islands was found in the paired strains. Transcriptome profiles showed little or no difference in the paired strains. In conclusion, the genome of A. actinomycetemcomitans appears to be relatively stable during short-term infection. Several types of genomic changes were observed in the paired strains of A. actinomycetemcomitans recovered from the same subjects, including a mutation in serotype-specific gene cluster that may allow the bacteria to evade host immune response.

  17. Withaferin A inhibits inflammatory responses induced by Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans in macrophages.

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    Noh, Eui-Jeong; Kang, Ming-Jung; Jeong, Yu-Jin; Lee, Jun-Young; Park, Jung-Hwan; Choi, Hye-Jin; Oh, Sang-Muk; Lee, Kyung-Bok; Kim, Dong-Jae; Shin, Ji-Ae; Cho, Sung-Dae; Park, Jong-Hwan

    2016-07-01

    Periodontitis is a progressive chronic inflammatory disease and a major cause of tooth loss in humans. As a withanolides, withaferin A (WA) is known to exhibit strong anti‑inflammatory activity. The present study examined whether WA inhibited inflammatory responses in macrophages in response to two representative periodontal pathogens, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans. Murine bone marrow‑derived macrophages (BMDMs) were used in this study and cytokine production in culture supernatants was measured by enzyme‑linked immunosorbent assays. Western blot analysis was performed to determine the activation of nuclear factor‑κB and mitogen‑activated protein kinases (MAPKs) and the expression of inducible nitric oxide synthase (iNOS), toll‑like receptor (TLR) 2 and TLR4. The production of nitric oxide (NO) was determined by the Griess reaction. WA treatment was shown to decrease interleukin (IL)‑6 and tumor necrosis factor (TNF)‑α production in BMDMs in response to F. nucleatum and A. actinomycetemcomitans in a dose‑dependent manner. The phosphorylation of IκB‑α and MAPKs (p38, extracellular signal‑regulated kinases and c‑Jun N‑terminal kinases) induced by F. nucleatum and A. actinomycetemcomitans was also inhibited by WA. F. nucleatum and A. actinomycetemcomitans induced iNOS expression and NO production in BMDMs, which was inhibited by WA in a dose‑dependent manner. WA also reduced endogenous and induced expression of TLR2 and TLR4 in these cells. These results suggest that WA may be a potential therapeutic agent or preventive additive for periodontitis control. PMID:27220676

  18. [Microbiological approach to a possible infective endocarditis case caused by Aggregatibacter actinomycetemcomitans].

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    Gürcan, Şaban; Ünlü, Selahattin; Kuloğlu, Figen; Karadenizli, Aynur; Kuşkucu, Mert Ahmet

    2016-04-01

    Aggregatibacter (Actinobacillus) actinomycetemcomitans, a small, gram-negative coccobacillus that grows slow and fastidious, is generally colonized in the oral cavity. It is a rarely seen bacterium because of the difficulty of isolation but it can be a causative agent for dental infections and infective endocarditis (IE) particularly in the persons having prosthetic heart valves. In this report, a possible IE case caused by A.actinomycetemcomitans in a patient with aortic valve replacement has been presented. A 36-year-old man has admitted to Trakya University Hospital, Health Center for Medical Research and Practice, with the complaints of chills, malaise, intermittent fever, severe arthralgia and weight loss (20 kg). During his follow-up period, the blood cultures that were obtained three week intervals yielded the identical gram-negative coccobacilli morphology. The patient was then diagnosed as possible IE on the basis of having one major (growth of the typical microorganisms that may cause IE in two different blood cultures) and two minor (presence of prosthetic valve and high fever) criterias. The isolate could not be identified with conventional methods, while it was identified as Francisella tularensis with VITEK 2 (bioMerieux, France) system. Hence this identification was not confirmed by real-time Taqman polymerase chain reaction, so MALDI-TOF mass spectrometry was used to identify this bacteria. In the first run of the study, the isolate was named as Shigella dysenteriae initially, however when it was retested the next day it was identified as A.actinomycetemcomitans. In order to enlighten these conflicting results, 16S and 23S ribosomal DNA sequence analysis was performed, and consequently the bacterium was identified as A.actinomycetemcomitans. Doxycycline (2 x 100 mg po, 20 days) and streptomycin (2 x 10 mg/kg im, 10 days) therapy were initiated, considering the initial suspicious identification (F.tularensis), and on the fifth day of therapy the

  19. Identification of a novel bacterial outer membrane interleukin-1Β-binding protein from Aggregatibacter actinomycetemcomitans.

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    Annamari Paino

    Full Text Available Aggregatibacter actinomycetemcomitans is a gram-negative opportunistic oral pathogen. It is frequently associated with subgingival biofilms of both chronic and aggressive periodontitis, and the diseased sites of the periodontium exhibit increased levels of the proinflammatory mediator interleukin (IL-1β. Some bacterial species can alter their physiological properties as a result of sensing IL-1β. We have recently shown that this cytokine localizes to the cytoplasm of A. actinomycetemcomitans in co-cultures with organotypic gingival mucosa. However, current knowledge about the mechanism underlying bacterial IL-1β sensing is still limited. In this study, we characterized the interaction of A. actinomycetemcomitans total membrane protein with IL-1β through electrophoretic mobility shift assays. The interacting protein, which we have designated bacterial interleukin receptor I (BilRI, was identified through mass spectrometry and was found to be Pasteurellaceae specific. Based on the results obtained using protein function prediction tools, this protein localizes to the outer membrane and contains a typical lipoprotein signal sequence. All six tested biofilm cultures of clinical A. actinomycetemcomitans strains expressed the protein according to phage display-derived antibody detection. Moreover, proteinase K treatment of whole A. actinomycetemcomitans cells eliminated BilRI forms that were outer membrane specific, as determined through immunoblotting. The protein was overexpressed in Escherichia coli in both the outer membrane-associated form and a soluble cytoplasmic form. When assessed using flow cytometry, the BilRI-overexpressing E. coli cells were observed to bind 2.5 times more biotinylated-IL-1β than the control cells, as detected with avidin-FITC. Overexpression of BilRI did not cause binding of a biotinylated negative control protein. In a microplate assay, soluble BilRI bound to IL-1β, but this binding was not specific, as a control

  20. Oil Essential Mouthwashes Antibacterial Activity against Aggregatibacter actinomycetemcomitans: A Comparison between Antibiofilm and Antiplanktonic Effects

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    Matteo Erriu

    2013-01-01

    Full Text Available The aim of this work is to determine the antibacterial activity of three marketed mouthwashes on suspended and sessile states of Aggregatibacter actinomycetemcomitans. The efficacy of two commonly used products in clinical practice, containing essential oils as active ingredients (menthol, thymol, methyl salicylate, and eucalyptol in association with or without alcohol, has been evaluated in comparison with a chlorhexidine-based mouthwash. The microtiter plate assay, in order to obtain a spectrophotometric measurement of bacterial responses at growing dilutions of each antiseptic, was used for the study. The analysis revealed that a good antibacterial activity is reached when the abovementioned mouthwashes were used at concentration over a 1/24 dilution and after an exposure time of 30 seconds at least. In conclusion, the alcoholic mouthwash appears to have a better biofilm inhibition than its antiplanktonic activity while the nonalcoholic product demonstrates an opposite effect with a better antiplanktonic behavior.

  1. Serotype-dependent expression patterns of stabilized lipopolysaccharide aggregates in Aggregatibacter actinomycetemcomitans strains.

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    Kikuchi, Haruko; Fujise, Osamu; Miura, Mayumi; Tanaka, Ayako; Hisano, Kyoko; Haraguchi, Akira; Hamachi, Takafumi; Maeda, Katsumasa

    2012-10-01

    Above a critical concentration, amphiphilic lipopolysaccharide (LPS) molecules in an aqueous environment form aggregate structures, probably because of interactions involving hydrophobic bonds. Ionic bonds involving divalent cations stabilize these aggregate structures, making them resistant to breakdown by detergents. The aim of this study was to examine expression patterns of stabilized LPS aggregates in Aggregatibacter actinomycetemcomitans, a microorganism that causes periodontitis. A. actinomycetemcomitans strains of various serotypes and truncated LPS mutants were prepared for this study. Following treatment with a two-phase separation system using the detergent Triton X-114, crude LPS extracts of the study strains were separated into detergent-phase LPS (DP-LPS) and aqueous-phase LPS (AP-LPS). Repeated treatment of the aqueous phase with the two-phase separation system produced only a slight decrease in AP-LPS, suggesting that AP-LPS was resistant to the detergent and thus distinguishable from DP-LPS. The presence of divalent cations increased the yield of AP-LPS. AP-LPS expression patterns were serotype-dependent; serotypes b and f showing early expression, and serotypes a and c late expression. In addition, highly truncated LPS from a waaD (rfaD) mutant were unable to generate AP-LPS, suggesting involvement of the LPS structure in the generation of AP-LPS. The two-phase separation was able to distinguish two types of LPS with different physical states at the supramolecular structure level. Hence, AP-LPS likely represents stabilized LPS aggregates, whereas DP-LPS might be derived from non-stabilized aggregates. Furthermore, time-dependent expression of stabilized LPS aggregates was found to be serotype-dependent in A. actinomycetemcomitans.

  2. Cytolethal distending toxin in isolates of Aggregatibacter actinomycetemcomitans from Ghanaian adolescents and association with serotype and disease progression.

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    Carola Höglund Åberg

    Full Text Available BACKGROUND AND OBJECTIVES: The cytolethal distending toxin (Cdt is a highly conserved exotoxin that are produced by a number of Gram negative bacteria, including Aggregatibacter actinomycetemcomitans, and affects mammalian cells by inhibiting cell division and causing apoptosis. A complete cdt-operon is present in the majority of A. actinomycetemcomitans, but the proportion of isolates that lack cdt-encoding genes (A, B and C varies according to the population studied. The objectives of this study were to examine serotype, Cdt-genotype, and Cdt-activity in isolates of A. actinomycetemcomitans collected from an adolescent West African population and to examine the association between the carrier status of A. actinomycetemcomitans and the progression of attachment loss (AL. MATERIALS AND METHODS: A total of 249 A. actinomycetemcomitans isolates from 200 Ghanaian adolescents were examined for serotype and cdt-genotype by PCR. The activity of the Cdt-toxin was examined by DNA-staining of exposed cultured cells and documented with flow cytometry. The periodontal status of the participants was examined at baseline and at a two-year follow-up. RESULTS: Presence of all three cdt-encoding genes was detected in 79% of the examined A. actinomycetemcomitans isolates. All these isolates showed a substantial Cdt-activity. The two different cdt-genotypes (with and without presence of all three cdt-encoding genes showed a serotype-dependent distribution pattern. Presence of A. actinomycetemcomitans was significantly associated with progression of AL (OR = 5.126; 95% CI = [2.994-8.779], p<0.001. CONCLUSION: A. actinomycetemcomitans isolated from the Ghanaian adolescents showed a distribution of serotype and cdt-genotype in line with results based on other previously studied populations. Presence of A. actinomycetemcomitans was significantly associated with disease progression, in particular the b serotype, whereas the association with disease progression

  3. Profound Effects of Aggregatibacter actinomycetemcomitans Leukotoxin Mutation on Adherence Properties Are Clarified in in vitro Experiments.

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    Senthil Kumar Velusamy

    Full Text Available Leukotoxin (Ltx is a prominent virulence factor produced by Aggregatibacter actinomycetemcomitans, an oral microorganism highly associated with aggressive periodontitis. Ltx compromises host responsiveness by altering the viability of neutrophils, lymphocytes, and macrophages. Previously, we developed a Rhesus (Rh monkey colonization model designed to determine the effect of virulence gene mutations on colonization of A. actinomycetemcomitans. Unexpectedly, an A. actinomycetemcomitans leukotoxin (ltxA mutant (RhAa-VS2 failed to colonize in the Rh model. No previous literature suggested that Ltx was associated with A. actinomycetemcomitans binding to tooth surfaces. These results led us to explore the broad effects of the ltxA mutation in vitro. Results indicated that LtxA activity was completely abolished in RhAa-VS2 strain, while complementation significantly (P<0.0001 restored leukotoxicity compared to RhAa-VS2 strain. RT-PCR analysis of ltx gene expression ruled out polar effects. Furthermore, binding of RhAa-VS2 to salivary-coated hydroxyapatite (SHA was significantly decreased (P<0.0001 compared to wild type RhAa3 strain. Real time RT-PCR analysis of the genes related to SHA binding in RhAa-VS2 showed that genes related to binding were downregulated [rcpA (P = 0.018, rcpB (P = 0.02, tadA (P = 0.002] as compared to wild type RhAa3. RhAa-VS2 also exhibited decreased biofilm depth (P = 0.008 and exo-polysaccharide production (P<0.0001. Buccal epithelial cell (BEC binding of RhAa-VS2 was unaffected. Complementation with ltxA restored binding to SHA (P<0.002 but had no effect on biofilm formation when compared to RhAa3. In conclusion, mutation of ltxA diminished hard tissue binding in vitro, which helps explain the previous in vivo failure of a ltxA knockout to colonize the Rh oral cavity. These results suggest that; 1 one specific gene knockout (in this case ltxA could affect other seemingly unrelated genes (such as rcpA, rcpB tadA etc, and 2

  4. Virulencia y variabilidad de Porphyromonas gingivalis y Aggregatibacter actinomycetemcomitans y su asociación a la periodontitis Virulence and variability on Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans and their association to periodontitis

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    J Díaz Zúñiga

    2012-04-01

    Full Text Available Las periodontitis son un conjunto de patologías de naturaleza inflamatoria y etiología infecciosa producidas por el biofilm patogénico subgingival. Porphyromonas gingivalis y Aggregatibacter actinomycetemcomitans son bacterias periodonto-patógenas que pueden causar daño directo a las estructuras periodontales a través de los diversos factores de virulencia que expresan. Sobre la base de estos factores de virulencia, distintos genotipos y serotipos bacterianos se han descrito, cada uno de ellos con una potencial variable patogenicidad. En esta revisión bibliográfica se describen diferentes factores de virulencia de P. gingivalis y A. actinomycetemcomitans y se discute la variable inmunogenicidad y patogenicidad de los distintos genotipos y serotipos descritos para ellos. Tanto P. gingivalis como A. actinomycetemcomitans poseen diversos factores de virulencia asociados al inicio, progresión y severidad de las periodontitis. En P. gingivalis, los factores de virulencia para los cuales se describen distintos genotipos y/o serotipos son fimbria, LPS y cápsula bacteriana, y en A. actinomycetemcomitans son leucotoxina A, Cdt y LPS. Cada uno de estos distintos genotipos y serotipos induce una respuesta inmuno-inflamatoria diferente en el hospedero y, por lo tanto, se podrían asociar a una variable patogenicidad y podrían determinar las características clínicas de la enfermedad.Periodontitis represents a heterogenic group of periodontal infections elicited by bacteria residing at the subgingival biofilm. Although this biofilm is constituted by a broad variety of bacterial species, only a limited number has been associated with the periodontitis aetiology, among them Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Both P. gingivalis and A. actinomycetemcomitans express a number of virulence factors that contribute to direct tissue damage and, based on them, distinct genotypes and serotypes have been described, each one

  5. Virulencia y variabilidad de Porphyromonas gingivalis y Aggregatibacter actinomycetemcomitans y su asociación a la periodontitis Virulence and variability on Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans and their association to periodontitis

    OpenAIRE

    J Díaz Zúñiga; J Yáñez Figueroa; S Melgar Rodríguez; C Álvarez Rivas; C Rojas Lagos; R Vernal Astudillo

    2012-01-01

    Las periodontitis son un conjunto de patologías de naturaleza inflamatoria y etiología infecciosa producidas por el biofilm patogénico subgingival. Porphyromonas gingivalis y Aggregatibacter actinomycetemcomitans son bacterias periodonto-patógenas que pueden causar daño directo a las estructuras periodontales a través de los diversos factores de virulencia que expresan. Sobre la base de estos factores de virulencia, distintos genotipos y serotipos bacterianos se han descrito, cada uno de ello...

  6. Detection of antimicrobial activity of banana peel (Musa paradisiaca L. on Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans: An in vitro study

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    Suraj Premal Kapadia

    2015-01-01

    Full Text Available Introduction and Aim: Banana is used widely because of its nutritional values. In past, there are studies that show banana plant parts, and their fruits can be used to treat the human diseases. Banana peel is a part of banana fruit that also has the antibacterial activity against microorganisms but has not been studied extensively. Since, there are no studies that relate the antibacterial activity of banana peel against periodontal pathogens. Hence, the aim of this study is to determine the antimicrobial activity of banana peel extract on Porphyromonas gingivalis (P. gingivalis and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans. Material and Methods: Standard strains of P. gingivalis and A. actinomycetemcomitans were used in this study which was obtained from the in-house bacterial bank of Department of Molecular Biology and Immunology at Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre. The banana peel extract was prepared, and the antibacterial activity was assessed using well agar diffusion method and minimum inhibitory concentration was assessed using serial broth dilution method. Results: In the current study, both the tested microorganisms showed antibacterial activity. In well diffusion method, P. gingivalis and A. actinomycetemcomitans showed 15 mm and 12 mm inhibition zone against an alcoholic extract of banana peel, respectively. In serial broth dilution method P. gingivalis and A. actinomycetemcomitans were sensitive until 31.25 μg/ml dilutions. Conclusion: From results of the study, it is suggested that an alcoholic extract of banana peel has antimicrobial activity against P. gingivalis and A. actinomycetemcomitans.

  7. The influence of adhesin protein from Aggregatibacter actinomycetemcomitans on IL-8 and MMP-8 titre in aggressive periodontitis

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    Rini Revijanti Ridwan

    2015-03-01

    Full Text Available Background: Adhesion can actually be considered as a part of both a powerful survival mechanism and a virulence mechanism for bacterial pathogens. Bacterial adhesin is an instrument for bacteria to do invasion to host. Bacterial adhesin depends on ligand interaction as a signaling mediator that will influence invasion and increase pro and anti-inflammatory because of the influence of the receptors of innate immune response. Aggregatibacter actimycetemcomitans has fimbriae included in type IV pili containing mostly with protein weighed 6.5 kDa and at least with protein weighed 54 kDa. Purpose: The purpose of this research is to analyze the influence of the induction of adhesin protein derived from A. actinomycetemcomitans on IL-8 and MMP-8 titre of Wistar rats. Methods: Adhesin protein derived from A. actinomycetemcomitans weighed 24 kDa was induced on the maxillary first molar sulcus of Wistar rats to prove that adhesin protein could affect IL-8 and MMP-8 titre. Next, to determine its influence, Elisa technique was conducted. Results: It is known that the levels of IL-8 and MMP-8 titre were increased in the group induced with adhesin protein derived from A. actinomycetemcomitans compared with the control group. Conclusion: It can be concluded that adhesin protein derived from A. actinomycetemcomitans can cause alveolar bone damage through the increasing levels of IL-8 and MMP-8 in aggressive periodontitis.

  8. Transcriptome Profiling of Wild-Type and pga-Knockout Mutant Strains Reveal the Role of Exopolysaccharide in Aggregatibacter actinomycetemcomitans.

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    Mayilvahanan Shanmugam

    Full Text Available Exopolysaccharides have a diverse set of functions in most bacteria including a mechanistic role in protecting bacteria against environmental stresses. Among the many functions attributed to the exopolysaccharides, biofilm formation, antibiotic resistance, immune evasion and colonization have been studied most extensively. The exopolysaccharide produced by many Gram positive as well as Gram negative bacteria including the oral pathogen Aggregatibacter actinomycetemcomitans is the homopolymer of β(1,6-linked N-acetylglucosamine. Recently, we reported that the PGA-deficient mutant of A. actinomycetemcomitans failed to colonize or induce bone resorption in a rat model of periodontal disease, and the colonization genes, apiA and aae, were significantly down regulated in the mutant strain. To understand the role of exopolysaccharide and the pga locus in the global expression of A. actinomycetemcomitans, we have used comparative transcriptome profiling to identify differentially expressed genes in the wild-type strain in relation to the PGA-deficient strain. Transcriptome analysis revealed that about 50% of the genes are differently expressed (P 1.5. Our study demonstrated that the absence of the pga locus affects the genes involved in peptidoglycan recycling, glycogen storage, and virulence. Further, using confocal microscopy and plating assays, we show that the viability of pga mutant strain is significantly reduced during biofilm growth. Thus, this study highlights the importance of pga genes and the exopolysaccharide in the virulence of A. actinomycetemcomitans.

  9. Occurrence of Aggregatibacter actinomycetemcomitans in Indian chronic periodontitis patients and periodontally healthy adults

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    Vinayak Mahableshwar Joshi

    2016-01-01

    Full Text Available Background: Aggregatibacter actinomycetemcomitans (Aa, an important primary periodontal pathogen, is known for its strong virulence characteristics that cause periodontal disease. We investigated Aa occurrence in Indian individuals using culture and 16 s rDNA polymerase chain reaction (PCR. Materials and Methods: A cross-sectional study with 100 participants each in the healthy and chronic periodontitis (CP groups was conducted. The subgingival plaque was collected and immediately plated on selective media for Aa. The remaining plaque samples were used for DNA extraction. PCR was performed using specific primers for Aa. Statistical Analysis Used: The detection of bacteria and the clinical parameters between the groups were compared using the Mann–Whitney U-test. For assessing the agreement between the results of anaerobic culture and PCR, Kappa analyses were performed. Results: Aa levels using culture and PCR was 51% and 69% in the CP group and 12% and 30% in the healthy group, respectively. The two groups showed significant differences (P < 0.00001. The detection accuracy of culture and PCR was assessed, and the coefficient of accuracy (k was highly significant in the healthy (0.3103; P < 0.0001 and CP groups (0.1536; P < 0.0497. Conclusions: Aa was predominantly found in the CP group compared with the healthy group, which is consistent with previous findings. Our results showed that both techniques can be used for detecting Aa. An ideal technique for detecting subgingival microorganisms should be carefully selected depending on the scope of the intended future work.

  10. Surface display of Aggregatibacter actinomycetemcomitans autotransporter Aae and dispersin B hybrid act as antibiofilm agents.

    Science.gov (United States)

    Ragunath, C; DiFranco, K; Shanmugam, M; Gopal, P; Vyas, V; Fine, D H; Cugini, C; Ramasubbu, N

    2016-08-01

    Among the various proteins expressed by the periodontopathogen Aggregatibacter actinomycetemcomitans, two proteins play important roles for survival in the oral cavity. The autotransporter Aae facilitates the attachment of the pathogen to oral epithelial cells, which act as a reservoir, while the biofilm-degrading glycoside hydrolase dispersin B facilitates the movement of daughter cells from the mature biofilm to a new site. The objective of this study was to use the potential of these two proteins to control biofilms. To this end, we generated a hybrid construct between the Aae C-terminal translocating domain and dispersin B, and mobilized it into Escherichia coli Rosetta (DE3) pLysS cells. Immunofluorescence analysis of the modified E. coli cells confirmed the presence of dispersin B on the surface. Further, the membrane localization of the displayed dispersin B was confirmed with Western blot analysis. The integrity of the E. coli cells displaying the dispersin B was confirmed through FACS analysis. The hydrolytic activity of the surface-displayed dispersin B was confirmed by using 4-methylumbelliferyl-β-d-glucopyranoside as the substrate. The detachment ability of the dispersin B surface-displaying E. coli cells was shown using Staphylococcus epidermidis and Actinobacillus pleuropneumoniae biofilms in a microtiter assay. We concluded that the Aae β-domain is sufficient to translocate foreign enzymes in the native folded form and that the method of Aae-mediated translocation of surface displayed enzymes might be useful for control of biofilms. PMID:26280561

  11. Determination of antibacterial activity of green coffee bean extract on periodontogenic bacteria like Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans: An in vitrostudy

    OpenAIRE

    Nagaraj Bharath; Nagur Karibasappa Sowmya; Dhoom Singh Mehta

    2015-01-01

    Background: The aim of this study was to evaluate the antibacterial activity of pure green coffee bean extract on periodonto pathogenic bacteria Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Fusobacterium nucleatum (Fn) and Aggregatibacter actinomycetemcomitans (Aa). Materials and Methods: Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBC) were used to assess the antibacterial effect of pure green coffee bean extract against periodonto pathogen...

  12. Detection of a 640-bp deletion in the Aggregatibacter actinomycetemcomitans leukotoxin promoter region in isolates from an adolescent of Ethiopian origin

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    Rolf Claesson

    2015-04-01

    Full Text Available The expression of the leukotoxin of Aggregatibacter actinomycetemcomitans is regulated by the leukotoxin promoter. A 530-bp deletion or an 886-bp insertion sequence (IS element in this region has earlier been described in highly leukotoxic isolates. Here, we report on highly leukotoxic isolate with a 640-bp deletion, which was detected in an adolescent of Ethiopian origin.

  13. Antibacterial effect of an herbal product persica on porphyromonas gingivalis and aggregatibacter actinomycetemcomitans: an in-vitro study.

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    Zahra Jelvehgaran Esfahani

    2014-08-01

    Full Text Available The plant Salvadora persica is used for oral hygiene in many parts of the world. It has been suggested that it has antibacterial properties, in addition to its ability to mechanically remove plaques. The aim of this study was to assess the antimicrobial activity of the herbal product Persica containing Salvadora persica against periodontopathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans in vitro.Fifty patients with moderate and severe periodontitis were recruited. Using paper points, subgingival plaque samples were taken from pockets with attachment loss ≥ 3mm. The samples were subjected to microbial culture to yield P. gingivalis and A. actinomycetemcomitans. The ditch plate method was used for antimicrobial susceptibility testing of the bacteria to Persica compared to chlorhexidine and distilled water. The growth inhibition zones of microorganisms around the ditches were measured in millimeters. The data were analyzed using SPSS 16. Freidman test and Wilcoxon signed ranks test with Bonferroni adjustment were used for analysis of variance with 5% significance level. P<0.05 for main comparisons and P< 0.017 for multiple comparisons were considered statistically significant.P. gingivalis was sensitive to chlorhexidine and persica. There was a significant difference (P=0.001 between antimicrobial activity of chlorhexidine (mean 28.733mm, SD 5.216 and Persica (mean 16.333mm, SD 5.259 compared to water against P. gingivalis. There was a significant difference (P< 0.001 between the antimicrobial activity of chlorhexidine (24.045mm, SD 3.897 and Persica (0.545mm, SD 2.558 with respect to A. actinomycetemcomitans. There was no significant difference (P=0.317 between the antimicrobial activity of Persica and water against A. actinomycetemcomitans.The herbal product Persica had significant antimicrobial activity against P. gingivalis and negligible antimicrobial activity against A. actinomycetemcomitans compared to 0

  14. ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF SYZYGIUM AROMATICUM (L. MERR.PERRY (CLOVES AGAINST CLINICAL ISOLATES OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS.

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    Ramanath Karicheri

    2015-05-01

    Full Text Available Aim: Aggregatibacter actinomycetemcomitans implicated in the etiology of aggressive, chronic periodontitis and other systemic infections. The aim of th is study was to investigate the antibacterial efficacy of essential oil of a traditional medicinal plant, Syzygium aromaticum ( L . Merr.perry (cloves against the clinical isolates of A.actinomycetemcomitans obtained from the patients with orodental infections. Methodology: Essential oil of clove flower buds was distilled by Neo-Clevenger’s method and the an tibacterial activity was tested by disc diffusion and agar punch well method. The minimum inhibitory concentration of clove oil was determined by micro broth dilution method. Results: 80.8% of the isolates showed sensitivity against cl ove oil by disc diffusion technique and the minimum inhibitory concentration of clove oil was ranged between 3.125μl/ml to 12.5μl/ml. Conclusion: Essential oil of Syzygium aromaticum showed good antibacterial activity and can be considered as alternative treatment option for control of periodontitis and other orodental infections caused by A.actinomycetemcomitans .

  15. The JP2 genotype of Aggregatibacter actinomycetemcomitans and marginal periodontitis in the mixed dentition

    DEFF Research Database (Denmark)

    Jensen, Anne Birkeholm; Ennibi, Oum Keltoum; Ismaili, Zouheir;

    2016-01-01

    AIM: To perform a cross-sectional study on the carrier frequency of JP2 and non-JP2 genotypes of A. actinomycetemcomitans in Moroccan schoolchildren and relate the presence of these genotypes to the periodontal status in the mixed dentition. MATERIAL AND METHODS: A plaque sample from 513 children...... the JP2 genotype and 186 (36.3%) were positive for non-JP2 genotypes, whereas A. actinomycetemcomitans could not be detected in the remaining 281 subjects. Among 75 subjects with mixed dentition and selected for clinical examination, clinical attachment loss (CAL) ≥3 mm at two or more periodontal sites...

  16. Efektivitas Ekstrak Kulit Buah Delima (Punica Granatum L.) Terhadap Bakteri Aggregatibacter Actinomycetemcomitans Secara In Vitro

    OpenAIRE

    Fitri, Arisma Dwita

    2015-01-01

    Aggregatibacter actinomycetemcomintans (Aa) is one type of pathogenic bacteria that cause periodontal disease found in plaque. The occurance of resistance of microorganisms to synthetic antibiotics and the resulting side effects of herbal ingredients make a good alternative to overcome these weaknesses. Pomegranate (Punica granatum L.) is known to have a wide range of therapeutic properties that antimicrobial, antioxidant, anticarcinogenic and antiinflammatory activity. The aim of this study ...

  17. Breaking the Gingival Epithelial Barrier: Role of the Aggregatibacter actinomycetemcomitans Cytolethal Distending Toxin in Oral Infectious Disease

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    Joseph M. DiRienzo

    2014-05-01

    Full Text Available The Gram-negative bacterium Aggregatibacter actinomycetemcomitans is part of the HACEK group that causes infective endocarditis, a constituent of the oral flora that promotes some forms of periodontal disease and a member of the family of species that secrete a cytolethal distending toxin (Cdt. The family of bacteria that express the cdt genes participate in diseases that involve the disruption of a mucosal or epithelial layer. In vitro studies have shown that human gingival epithelial cells (HGEC are native targets of the Cdt that typically induces DNA damage that signals growth arrest at the G2/M interphase of the cell cycle. The gingival epithelium is an early line of defense in the oral cavity against microbial assault. When damaged, bacteria collectively gain entry into the underlying connective tissue where microbial products can affect processes and pathways in infiltrating inflammatory cells culminating in the destruction of the attachment apparatus of the tooth. One approach has been the use of an ex vivo gingival explant model to assess the effects of the Cdt on the morphology and integrity of the tissue. The goal of this review is to provide an overview of these studies and to critically examine the potential contribution of the Cdt to the breakdown of the protective gingival barrier.

  18. A new functional site W115 in CdtA is critical for Aggregatibacter actinomycetemcomitans cytolethal distending toxin.

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    Lu Li

    Full Text Available Aggregatibacter actinomycetemcomitans, a specific pathogen of localized aggressive periodontitis, produces a cytolethal distending toxin (CDT that arrests eukaryotic cells irreversibly in G0/G1 or G2/M phase of the cell cycle. Although structural studies show that the aromatic patch region of CdtA plays an important role in its biological activity, the functional sites of CdtA have not been firmly established. In this study, site-specific mutagenesis strategy was employed for cdtA point mutations construction so as to examine the contributions of individual amino acids to receptor binding and the biological activity of holotoxin. The binding ability was reduced in CdtA(Y181ABC holotoxin and the biological function of CDT was not weaken in CdtA(Y105ABC, CdtA(Y125ABC, CdtA(F109ABC and CdtA(S106NBC holotoxin suggesting that these sites were not critical to CDT. But the binding activity and cell cycle arrest ability of holotoxin complexes were inhibited in CdtA(W115GBC. And this site did not affect the holotoxin assembly by size exclusion chromatography. Therefore, W115 might be a critical site of CdtA binding ability. These findings suggest that the functional sites of CdtA are not only in the aromatic patch region. W115, the new functional site is critical for receptor binding and cell cycle arrest, which provides potential targets for pharmacological disruption of CDT activity.

  19. Al(III), Pd(II), and Zn(II) phthalocyanines for inactivation of dental pathogen Aggregatibacter actinomycetemcomitans as planktonic and biofilm-cultures

    Science.gov (United States)

    Kussovski, V.; Mantareva, V.; Angelov, I.; Avramov, L.; Popova, E.; Dimitrov, S.

    2012-06-01

    The Gram-negative, oral bacterium Aggregatibacter actinomycetemcomitans has been implicated as the causative agent of several forms of periodontal disease in humans. The new periodontal disease treatments are emergence in order to prevent infection progression. Antimicrobial photodynamic therapy (a-PDT) can be a useful tool for this purpose. It involves the use of light of specific wavelength to activate a nontoxic photosensitizing agent in the presence of oxygen for eradication of target cells, and appears effective in photoinactivation of microorganisms. The phthalocyanine metal complexes of Pd(II)- (PdPcC) and Al(III)- (AlPc1) were evaluated as photodynamic sensitizers towards a dental pathogen A. actinomycetemcomitans in comparison to the known methylpyridyloxy-substituted Zn(II) phthalocyanine (ZnPcMe). The planktonic and biofilm-cultivated species of A. actinomycetemcomitans were treated. The photophysical results showed intensive and far-red absorbance with high tendency of aggregation for Pd(II)-phthalocyanine. The dark toxicities of both photosensitizers were negligible at concentrations used (bacterial biofilms were more resistant to a-PDT, which was confirmed for A. actinomycetemcomitans biofilms with 3 log reductions of viable cells after treatment with ZnPcMe and approximately 1 log reduction of biofilms after PdPcC and AlPc1. The initial results suggest that a-PDT can be useful for effective inactivation of dental pathogen A. actinomycetemcomitans.

  20. Al(III), Pd(II), and Zn(II) phthalocyanines for inactivation of dental pathogen Aggregatibacter actinomycetemcomitans as planktonic and biofilm-cultures

    Science.gov (United States)

    Kussovski, V.; Mantareva, V.; Angelov, I.; Avramov, L.; Popova, E.; Dimitrov, S.

    2012-06-01

    The Gram-negative, oral bacterium Aggregatibacter actinomycetemcomitans has been implicated as the causative agent of several forms of periodontal disease in humans. The new periodontal disease treatments are emergence in order to prevent infection progression. Antimicrobial photodynamic therapy (a-PDT) can be a useful tool for this purpose. It involves the use of light of specific wavelength to activate a nontoxic photosensitizing agent in the presence of oxygen for eradication of target cells, and appears effective in photoinactivation of microorganisms. The phthalocyanine metal complexes of Pd(II)- (PdPcC) and Al(III)- (AlPc1) were evaluated as photodynamic sensitizers towards a dental pathogen A. actinomycetemcomitans in comparison to the known methylpyridyloxy-substituted Zn(II) phthalocyanine (ZnPcMe). The planktonic and biofilm-cultivated species of A. actinomycetemcomitans were treated. The photophysical results showed intensive and far-red absorbance with high tendency of aggregation for Pd(II)-phthalocyanine. The dark toxicities of both photosensitizers were negligible at concentrations used (biofilms were more resistant to a-PDT, which was confirmed for A. actinomycetemcomitans biofilms with 3 log reductions of viable cells after treatment with ZnPcMe and approximately 1 log reduction of biofilms after PdPcC and AlPc1. The initial results suggest that a-PDT can be useful for effective inactivation of dental pathogen A. actinomycetemcomitans.

  1. Microevolution and Patterns of Dissemination of the JP2 Clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans

    DEFF Research Database (Denmark)

    Haubek, Dorte; Poulsen, Knud; Kilian, Mogens

    2007-01-01

    belonging to the JP2 clone had a number of point mutations, particularly in the pseudogenes hbpA and tbpA. Characteristic mutations allowed isolates from individuals from the Mediterranean area and from West Africa, including the Cape Verde Islands, to be distinguished. The patterns of mutations indicate...... in adolescents of African descent and differs from other clones of the species by several genetic peculiarities, including a 530-bp deletion in the promoter region of the leukotoxin gene operon, which results in increased leukotoxic activity. Multilocus sequence analysis of 82 A. actinomycetemcomitans strains...... of individuals of African descent despite geographical separation for centuries suggests that the JP2 clone has a distinct host tropism. The colonization of family members by JP2 clone strains with unique point mutations provides strong evidence that there is intrafamilial transmission and suggests...

  2. Distribution of biotypes and leukotoxic activity of Aggregatibacter actinomycetemcomitans isolated from Brazilian patients with chronic periodontitis Distribuição de biótipos e atividade leucotóxica de Aggregatibacter actinomycetemcomitans isolados de pacientes brasileiros com periodontite crônica

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    Elerson Gaetti-Jardim Jr.

    2008-12-01

    Full Text Available Aggregatibacter actinomycetemcomitans is an important etiologic agent of the periodontitis and is associated with extra-oral infections. In this study, the detection of the ltxA gene as well as the ltx promoter region from leukotoxic A. actinomycetemcomitans isolated from 50 Brazilian patients with periodontitis and 50 healthy subjects was performed. The leukotoxic activity on HL-60 cells was also evaluated. Leukotoxic activity was determined using a trypan blue exclusion method. The 530 bp deletion in the promoter region was evaluated by PCR using a PRO primer pair. A. actinomycetemcomitans was detected by culture and directly from crude subgingival biofilm by PCR using specific primers. By culture, A. actinomycetemcomitans was detected in nine (18% of the periodontal patients and one (2% healthy subject. However, by PCR, this organism was detected in 44% of the periodontal patients and in 16% of the healthy subjects. It was verified a great discrepancy between PCR detection of the ltx operon promoter directly from crude subgingival biofilm and from bacterial DNA. Only one periodontal sample harbored highly leukotoxic A. actinomycetemcomitans. Moreover, biotype II was the most prevalent and no correlation between biotypes and leukotoxic activity was observed. The diversity of leukotoxin expression by A. actinomycetemcomitans suggests a role of this toxin in the pathogenesis of periodontal disease and other infectious diseases.Aggregatibacter actinomycetemcomitans é um importante agente etiológico da periodontite e produz infecções extra-bucais. Neste estudo, foram detectados os biótipos, o gene ltxA associado à produção de leucotoxina e o promotor ltx em A. actinomycetemcomitans de pacientes com e sem periodontite. A atividade leucotóxica sobre células HL-60 também foi avaliada. A atividade leucotóxica foi determinada através do método de exclusão do azul de tripam. A deleção de 530 bp no promotor ltx foi avaliada usando-se o

  3. Determination of antibacterial activity of green coffee bean extract on periodontogenic bacteria like Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans: An in vitrostudy

    Directory of Open Access Journals (Sweden)

    Nagaraj Bharath

    2015-01-01

    Full Text Available Background: The aim of this study was to evaluate the antibacterial activity of pure green coffee bean extract on periodonto pathogenic bacteria Porphyromonas gingivalis (Pg, Prevotella intermedia (Pi, Fusobacterium nucleatum (Fn and Aggregatibacter actinomycetemcomitans (Aa. Materials and Methods: Minimum inhibitory concentrations (MICs and minimum bactericidal concentrations (MBC were used to assess the antibacterial effect of pure green coffee bean extract against periodonto pathogenic bacteria by micro dilution method and culture method, respectively. Results: MIC values of Pg, Pi and Aa were 0.2 μg/ml whereas Fn showed sensitive at concentration of 3.125 μg/ml. MBC values mirrors the values same as that of MIC. Conclusion: Antimicrobial activity of pure green coffee bean extract against Pg, Pi, Fn and Aa suggests that it could be recommended as an adjunct to mechanical therapy in the management of periodontal disease.

  4. Presencia de Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola y Aggregatibacter actinomycetemcomitans en el biofilm subgingival de pacientes diabéticos tipo 2: estudio transversal Presence of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Aggregatibacter actinomycetemcomitans in the subgingival biofilm of diabetic mellitus 2 patients: a cross sectional study

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    AJ Quintero

    2011-08-01

    Full Text Available Antecedentes: La investigación de la microflora subgingival en pacientes diabéticos tipo 2 con periodontitis ha presentado resultados contradictorios. Objetivo: Determinar la presencia de Porphyromonas gingivalis, Tannerella forshytia, Treponema denticola y Aggregatibacter actinomycetemcomitans, en el biofilm subgingival de pacientes diabéticos tipo 2 y relacionarlo con el grado de control metabólico. Método: Estudio descriptivo transversal, en el cual se analizaron 23 pacientes diabéticos derivados consecutivamente del Policlínico de Especialidades de la Universidad de los Andes. Previo consentimiento informado, se realizó un examen clínico periodontal que incluyó mediciones de profundidad al sondaje, nivel de inserción clínica y sangrado gingival. Fueron clasificados según severidad de periodontitis y control metabólico de la diabetes determinado por un promedio de 3 exámenes de hemoglobina glicosilada. La detección microbiológica se realizó mediante la técnica de reacción en cadena de la polimerasa. Resultados: En el grupo de pacientes estudiados, Treponema denticola y Tannerella forsythia fueron las bacterias más prevalentes (65.2%, seguida por Porphyromonas gingivalis (17.3% y Aggregatibacter actinomycetemcomitans (13%. Los pacientes con peor control glicémico tuvieron una mayor presencia de Treponema denticola, Tannerella forsythia, Porphyromonas gingivalis y Agreggatibacter actinomycetemcomitans y un aumento en el índice de sangrado al sondaje. Conclusiones: En el grupo de pacientes diabéticos estudiado, las bacterias más prevalentes fueron Treponema denticola y Tannerella forsythia. Los pacientes diabéticos tipo 2 con moderado y mal control glicémico presentaron mayor presencia de los microorganismos estudiados, comparado con los grupos con mejores niveles de control glicémico.Background: The investigation of subgingival microflora in type 2 diabetic patients with periodontitis presented conflicting results

  5. Determination of the antibacterial activity of simvastatin against periodontal pathogens, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans: An in vitro study

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    Shilpa Emani

    2014-01-01

    Full Text Available Context and Objective: Statin treatment, apart from its hypolipidemic action has proven its antimicrobial activity by improving the survival rate of patients with severe systemic bacterial infections. Periodontitis is an inflammatory disorder of tooth supporting structures caused by a group of specific microorganisms. The objective of the present study was to determine the antimicrobial activity of pure simvastatin drug against the primary periodontal pathogens. Materials and Methods: Minimum inhibitory concentration (MIC was determined against Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans using serial dilution method. Results: MIC of simvastatin against P. gingivalis was 2 μg/ml and A. actinomycetemcomitans was found to be <1 μg/ml which requires further dilutions to determine the exact value. Conclusions: Data suggests a potent antimicrobial activity of simvastatin against both A. actinomycetemcomitans and P gingivalis. Hence simvastatin can be prescribed as a dual action drug in patients with both hyperlipidemia and periodontal disease.

  6. Detection of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans after Systemic Administration of Amoxicillin Plus Metronidazole as an Adjunct to Non-surgical Periodontal Therapy: A Systematic Review and Meta-Analysis

    Science.gov (United States)

    Dakic, Aleksandar; Boillot, Adrien; Colliot, Cyrille; Carra, Maria-Clotilde; Czernichow, Sébastien; Bouchard, Philippe

    2016-01-01

    Objective: To evaluate the variations in the detection of Porphyromonas gingivalis and/or Aggregatibacter actinomycetemcomitans before and after systemic administration of amoxicillin plus metronidazole in association with non-surgical periodontal therapy (NSPT). Background: The adjunctive use of antibiotics has been advocated to improve the clinical outcomes of NSPT. However, no systematic review has investigated the microbiological benefit of this combination. Materials and Methods: An electronic search was conducted up to December 2015. Randomized clinical trials comparing the number of patients testing positive for P. gingivalis and/or A. actinomycetemcomitans before and after NSPT with (test group) or without (control group) amoxicillin plus metronidazole were included. The difference between groups in the variation of positive patients was calculated using the inverse variance method with a random effects model. Results: The frequency of patients positive for A. actinomycetemcomitans was decreased by 30% (p = 0.002) and by 25% (p = 0.01) in the test group compared to the control group at 3- and 6-month follow-up, respectively. Similar findings were observed when considering the frequency of patients positive for Porphyromonas gingivalis, with a reduction by 28% (p periodontal therapy alone or with a placebo. PMID:27594851

  7. Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Treponema denticola / Prevotella intermedia Co-Infection Are Associated with Severe Periodontitis in a Thai Population.

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    Kitti Torrungruang

    Full Text Available Periodontitis is a polymicrobial infection of tooth-supporting tissues. This cross-sectional study aimed to examine the associations between five target species and severe periodontitis in a Thai population. Using the CDC/AAP case definition, individuals diagnosed with no/mild and severe periodontitis were included. Quantitative analyses of Aggregatibacter actinomycetemcomitans (Aa, Porphyromonas gingivalis (Pg, Tannerella forsythia (Tf, Treponema denticola (Td, and Prevotella intermedia (Pi in subgingival plaque were performed using real-time polymerase chain reaction. The association between target species and severe periodontitis was examined using logistic regression analysis. The study subjects comprised 479 individuals with no/mild periodontitis and 883 with severe periodontitis. Bacterial prevalence and quantity were higher in subjects with severe periodontitis than in those with no/mild disease. In the fully adjusted model, all species except Tf showed a dose-dependent relationship with periodontitis. The mere presence of Pg, even in low amount, was significantly associated with severe periodontitis, while the amount of Aa, Td, and Pi had to reach the critical thresholds to be significantly associated with disease. Compared to individuals with low levels of both Td and Pi, high colonization by either Td or Pi alone significantly increased the odds of having severe periodontitis by 2.5 (95%CI 1.7-3.5 folds. The odds ratio was further increased to 14.8 (95%CI 9.2-23.8 in individuals who were highly colonized by both species. Moreover, the presence of Pg and high colonization by Aa were independently associated with severe periodontitis with odds ratios of 5.6 (95%CI 3.4-9.1 and 2.2 (95%CI 1.5-3.3, respectively. Our findings suggest that the presence of Pg and high colonization by Aa, Td, and Pi play an important role in severe periodontitis in this study population. We also demonstrate for the first time that individuals co-infected with

  8. Electrophoresis of phosphorylcholine epitope in Aggregatibacter actinomycetemcomitans%伴放线放线杆菌磷酸胆碱的电泳分析

    Institute of Scientific and Technical Information of China (English)

    钟德钰; 章锦才; 张雄; 葛春旭

    2012-01-01

    Objective To find out the chemical composition of the antigen reacting with TEPC-15 in Aggregatibacter actinomycetemcomitans (.4a), and the electrophoresis changes of the proteins and anti-TEPC-15 reactivity with and without proteinase K treatment were compared. Methods Bacteria cells were broken by Branson Sonifier, treated with pro-teinase K and separated on SDS-PAGE. The gels were first analyzed by Coomassie blue staining. Then the electrophoresis results were transferred onto membrane and immunoblot were done by using mouse anti-phosphorylcholine monoclonal antibody TEPC-15 as the primary antiserum and goat anti-mouse IgA as the secondary antiserum. Results After Coomassie blue staining, the sample without proteinase K treatment showed a variety of bands distributed from low to high molecular weight area. The samples treated with proteinase K revealed a single clear band of 29 kDa, which was not detected in the samples without treatment. This 29-kDa band could also be detected in a control sample with the same amount of proteinase K in PBS but no bacteria. Since the molecular size of proteinase K (Sigma) is 28.93 kDa, the result suggests that proteinase K was the source of the 29-kDa band. Immunoblot analysis showed a single band of 9 kDa with anti-TEPC-15 reactivity without proteinase K treatment and the proteinase K treatment eliminated the TEPC-15 reactivity with A. actino-mycetemcomitans lysates. Conclusion The Aa antigen for TEPC-15 is a protein, since proteinase K treatment of the cell lysate abolished the reactivity.%目的 比较3个磷酸胆碱阳性的伴放线放线杆菌菌株在蛋白酶K作用后电泳结果的变化,分析磷酸胆碱抗原在细菌中的附着结构.方法 将培养收集的伴放线放线杆菌破碎处理后,加入蛋白酶K水解,通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate polyacrylamide gel electrophoresis,SDS-PAGE),考玛斯亮蓝染色显示蛋白条带的分布情况,免疫印迹法检

  9. 侵袭性牙周炎患者抗伴放线聚集杆菌血清c型IgG滴度分析%Analysis of serum IgG titers to Aggregatibacter actinomycetemcomitans serotype c in aggressive periodontitis patients

    Institute of Scientific and Technical Information of China (English)

    冯向辉; 张立; 徐莉; 孟焕新; 陈智滨; 释栋; 路瑞芳

    2015-01-01

    目的:分析侵袭性牙周炎(aggressive periodontitis, AgP)患者血清中的抗伴放线聚集杆菌(Aggregatibacter actinomycetemcomitans, Aa)血清c型IgG抗体滴度及其影响因素。方法:采集62例AgP患者(18例切磨牙型,44例广泛型)和45例牙周健康者的空腹静脉血,同时收集AgP患者的龈下菌斑和非刺激性全唾液用于Aa的检测( PCR方法),应用酶联免疫吸附方法测定血清中抗Aa血清c型IgG抗体滴度。结果:AgP组和健康对照者血清中的抗Aa血清c型IgG抗体检出率均为100%, AgP 组的抗体滴度为11.1±1.9,显著高于健康对照组(9.1±1.8, P<0.01)。切磨牙型AgP患者的抗Aa血清c型的IgG抗体滴度和抗体升高率与广泛型比较差异无统计学意义。唾液或龈下菌斑样本 Aa 检出阳性的 AgP 患者抗体滴度显著高于Aa 阴性患者(11.9±1.3 vs.10.7±2.1, P<0.05)。结论:Aa血清c型为我国AgP患者定植的Aa的重要血清型,广泛型AgP患者血清抗Aa抗体滴度与切磨牙型AgP患者无明显差异。%Objective:To analyze the serum IgG titers to Aggregatibacter actinomycetemcomitans ( Aa ) and associated factors in patients with aggressive periodontitis (AgP).Methods:Venous blood samples were collected from 62 AgP patients and 45 periodontal healthy controls , unstimulated whole saliva and pooled subgingival plaque samples of AgP patients were also collected for the detection of Aa ( PCR method) .Serum IgG titers to Aa serotype c were measured by enzyme-linked immunosorbnent assay ( ELISA) .Results:The detection rates of serum IgG to Aa serotype c in the AgP patients and the healthy controls were both 100%.The AgP patients exhibited significantly higher IgG titers to Aa serotype c than the healthy controls (11.1 ±1.9 vs.9.1 ±1.8, P<0.01).There was no significant difference in serum IgG levels to Aa serotype c and in the prevalence of high-responding patients to Aa

  10. [Chemical structure and immunomodulating activities of peptidoglycan from Actinobacillus actinomycetemcomitans].

    Science.gov (United States)

    Kinoshita, F

    1989-12-01

    The chemical structure and immunomodulating activities of the cell wall peptidoglycans isolated from Actinobacillus actinomycetemcomitans were investigated. Peptidoglycans were isolated from A. actinomycetemcomitans strains Y4 and ATCC 29522 by boiling in 4% sodium dodecyl sulfate and by digestion with pronase, trypsin and alpha-amylase. Analysis of amino acids and amino sugars of the peptidoglycans revealed that glucosamine, muramic acid, D-glutamic acid, D-alanine, and meso-2, 6-diaminopimelic acid (A2pm) were the principal components. Serine and glycine were not found. Dinitrophenylation method revealed that about half of A2pm residue had a free aminogroup, and analysis by hydrazinolysis showed that a small part of alanine and A2pm located at the C-terminal. The above results indicate that one of the amino groups of A2pm residue at one strand of the stem peptide subunit crosslinked to the carboxyl group of alanine of the neighboring strand. It was thus revealed that the peptidoglycans of A. actinomycetemcomitans belonged to the Al gamma type of the classification by Schleifer and Kandler. Peptidoglycans isolated from A. actinomycetemcmitans strain Y4 and ATCC 29522 were found to be definitely adjuvant-active in induction of delayed type hypersensitivity against ovalbumin when administered to guinea pigs as water-in oil emulsion and stimulation of increase serum antibody levels was found in both peptidoglycans. Regarding mitogenicity on splenocytes of BALB/c and BALB/c nu/nu mice, peptidoglycans from two strains of A. actinomycetemcomitans were markedly enhanced the uptake [3H] thymidine in dose of 10 micrograms/10(5) cells, however thymocytes were not reactive. Stimulation effects on peritoneal macrophages from a guinea pig to incorporation of 14C-glucosamin were not exhibited on addition of 100 micrograms of both peptidoglycans. These findings indicate that peptidoglycan of A. actinomycetemcomitans might eventually be responsible for destruction of periodontal

  11. Anticorpos antileucotoxina contra Actinobacillus actinomycetemcomitans em amostras de soro e saliva de pacientes com periodontite juvenil localizada Anti-leukotoxin antibodies against Actinobacillus actinomycetemcomitans in serum and saliva samples from patients with localized juvenile periodontitis

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    Roberto Issamu NAKAGAWA

    2001-03-01

    Full Text Available A leucotoxina de Actinobacillus actinomycetemcomitans é considerada seu principal fator de virulência com potencial de causar agressão às defesas do hospedeiro. No presente trabalho, foram analisados os níveis séricos e salivares de anticorpos antileucotoxina de A. actinomycetemcomitans em soros e salivas de pacientes com periodontite juvenil localizada (PJL e controles saudáveis. Adicionalmente, foi realizada a análise de complexo imune (CI nas amostras de saliva. Foram utilizados os métodos ELISA clássico com a leucotoxina obtida por gel filtração em Sephadex G-200 e ELISA de captura utilizando IgG de coelho anti-A. actinomycetemcomitans FDC Y4 leucotóxico adsorvido com uma cepa da mesma espécie, porém, não leucotóxica. Os resultados obtidos demonstraram níveis séricos de IgG significativamente mais elevados em pacientes com PJL em relação aos controles sadios, tanto por ELISA clássico como por ELISA de captura (p The leukotoxin produced by Actinobacillus actinomycetemcomitans is considered the major virulence factor with potential to cause damage to the host defenses. The present work analyzed the serumal and salivary levels of antibodies against the leukotoxin produced by A. Actinomycetemcomitans, in patients with Localized Juvenile Periodontitis (LJP and in healthy controls. Additionally, analysis of the immune complex (IC was carried out in saliva samples . The classic ELISA method, with leukotoxin obtained through Sephadex G-200 gel filtration, and the capture ELISA method, using rabbit anti-A. Actinomycetemcomitans (leucotoxic, FDC Y4, IgG adsorbed with a non-leukotoxic strain of A. actinomycetemcomitans, were used. The results obtained demonstrated significantly higher serumal levels of IgG in patients with LJP, when they were compared with the healthy controls, both for the classic and capture ELISA methods (p < 0.05. However, no significant differences were observed between the salivary levels of IgG, SIgA and IC

  12. Aggregatibacter actinomycetemcomitansarcB influences hydrophobic properties, biofilm formation and adhesion to hydroxyapatite ArcB em Aggregatibacter actinomycetmcomitans influencia propriedades hidrofóbicas, formação de biofilme e aderência a hidroxiapatita

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    PL Longo

    2009-09-01

    Full Text Available The regulation of gene expression in the oral pathogen Aggregatibacter actinomycetemcomitans is still not fully elucidated. ArcAB is a two-component system which allows facultative anaerobic bacteria to sense various respiratory growth conditions and adapt their gene expression accordingly. This study investigated in A. actinomycetemcomitans the role of arcB on the regulation of biofilm formation, adhesion to saliva coated hydroxyapatite (SHA and the hydrophobic properties of the cell. These phenotypic traits were determined for an A. actinomycetemcomitansarcB deficient type and a wild type strain. Differences in hydrophobic properties were shown at early and late exponential growth phases under microaerobic incubation and at late exponential phase under anaerobiosis. The arcB mutant formed less biofilm than the wild type strain when grown under anaerobic incubation, but displayed higher biofilm formation activity under microaerobic conditions. The adherence to SHA was significantly lower in the mutant when compared with the wild type strain. These results suggest that the transmembrane sensor kinase arcB, in A. actinomycetemcomitans, senses redox growth conditions and regulates the expression of surface components of the bacterial cell related to biofilm formation and adhesion to saliva coated surfaces.A regulação da expressão gênica do patógeno oral Aggregatibacter actinomycetemcomitans não está completamente descrita. O sistema de dois componentes ArcAB permite que bactérias anaeróbias facultativas percebam diferenças nas condições respiratórias durante sua multiplicação e adaptem a expressão de genes à estas condições. Este estudo investigou em A. actinomycetemcomitans o papel de arcB na regulação da formação de biofilme, aderência à hidroxiapatita recoberta por saliva (SHA e nas propriedades hidrofóbicas celulares. Estas características fenotípicas foram determinadas para uma linhagem de A. actinomycetemcomitans

  13. Monodisperse and LPS-free Aggregatibacter actinomycetemcomitans leukotoxin: Interactions with human β2 integrins and erythrocytes

    DEFF Research Database (Denmark)

    Reinholdt, Jesper; Poulsen, Knud; Brinkmann, Christel Rothe;

    2013-01-01

    phagocytes. The known receptor for LtxA on leukocytes is integrin αLβ2 (LFA-1 or CD11a/CD18). However, the molecular mechanisms involved in LtxA-mediated cytotoxicity are poorly understood, partly because LtxA has proven difficult to prepare for experiments as free of contaminants and with its native...

  14. Adhesion of Actinobacillus actinomycetemcomitans to a human oral cell line.

    OpenAIRE

    Mintz, K. P.; Fives-Taylor, P M

    1994-01-01

    Two quantitative, rapid assays were developed to study the adhesion of Actinobacillus actinomycetemcomitans, an oral bacterium associated with periodontal disease, to human epithelial cells. The human oral carcinoma cell line KB was grown in microtiter plates, and adherent bacteria were detected by an enzyme-linked immunosorbent assay with purified anti-A. actinomycetemcomitans serum and horseradish peroxidase-conjugated secondary antibody or [3H]thymidine-labeled bacteria. Adhesion was found...

  15. LEUKOTOXIC ACTIVITY OF ACTINOBACILLUS ACTINOMYCETEMCOMITANS ISOLATED FROM HUMAN AND NON-HUMAN PRIMATES Atividade leucotóxica de amostras de Actinobacillus actinomycetemcomitans de primatas humanos não-humanos

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    Francisca Lúcia de Lima

    2001-10-01

    Full Text Available Actinobacillus actinomycetemcomitans is a clinically relevant periodontopathogenic Gram-negative coccobacillus that produces a leukotoxin of the RTX cytolysin family. In this study, we evaluated the leukotoxic activity of A. actinomycetemcomitans strains isolated from human and marmosets by Trypan blue exclusion and by the chemiluminescence assays. Among eight A. actinomycetemcomitans human strains studied, two (P2.17 and P8.12 were classified as high leukotoxin producers and among eight marmoset strains, one (M22.11 showed high leukotoxin production, as determined by Trypan blue exclusion assay. The reference strains ATCC 29523 and FDC Y4 respectively behaved like moderate and low producers. The chemiluminescence assay was used to evaluate the leukotoxic activity of M22.11 and P2.17 strains submitted to different growth conditions. Leukotoxic activity was detected on cells at the logarithmic phase and was similar under anaerobic and microaerophilic growth conditions. It was greatly reduced when cells were grown at glucose concentrations lower or higher than 0.75% (0.25% and 1.5% in thioglycolate medium. Leukotoxin production mainly by the M22.11 strain was low in BHI broth, whereas production in TSB medium showed a similar level as in thioglycolate broth medium. Sodium bicarbonate at 10 mM did not affect leukotoxin production.Actinobacillus actinomycetemcomitans é um cocobacilo Gram negativo, periodontopatógeno clinicamente importante, que produz uma leucotoxina pertencente à família das citolisinas RTX. Neste estudo, avaliou-se a atividade leucotóxica de amostras de A. actinomycetemcomitans isoladas de seres humanos e de calitriquídeos pelos métodos de exclusão de azul de Tripan e quimioluminescência. Duas (P2.17 e P8.12 entre oito amostras de A. actinomycetemcomitans isoladas de seres humanos, e uma (M22.11 entre 8 amostras isoladas de sagüis se apresentaram como altamente produtoras de leucotoxina, como determinado pelo teste de

  16. Nitric oxide production by murine spleen cells stimulated with lipopolysaccharide from Actinobacillus actinomycetemcomitans.

    Science.gov (United States)

    Sosroseno, Wihaskoro; Herminajeng, Endang; Susilowati, Heni; Budiarti, Sri

    2002-12-01

    The aim of this study was to determine whether Actinobacillus actinomycetemcomitans lipopolysaccharide (LPS-A. actinomycetemcomitans) could induce murine spleen cells to produce nitric oxide (NO). Spleen cells derived from Balb/c mice were stimulated with LPS-A. actinomycetemcomitans or LPS from Escherichia coli for 4 days. The effects of N(G)-monomethyl-L-arginine (NMMA), polymyxin B, and cytokines (IFN-gamma and IL-4) on the production of NO were also assessed. The NO production from the carrageenan-treated spleen cells stimulated with LPS-A. actinomycetemcomitans or both LPS-A. actinomycetemcomitans and IFN-gamma was determined. The carrageenan-treated mice were transferred with splenic macrophages and the NO production was assessed from the spleen cells stimulated with LPS-A. actinomycetemcomitans or LPS-A. actinomycetemcomitans and IFN-gamma. The results showed that NO production was detectable in the cultures of spleen cells stimulated with LPS-A. actinomycetemcomitans in a dose-dependent fashion, but was lower than in the cells stimulated with LPS from E. coli. The NO production was blocked by NMMA and polymyxin B. IFN-gamma up-regulated but IL-4 suppressed the production of NO by the spleen cells stimulated with LPS-A. actinomycetemcomitans. The carrageenan-treated spleen cells failed to produce NO after stimulation with LPS-A. actinomycetemcomitans or both LPS-A. actinomycetemcomitans and IFN-gamma. Adoptive transfer of splenic macrophages to the carrageenan-treated mice could restore the ability of the spleen cells to produce NO. The results of the present study suggest that LPS-A. actinomycetemcomitans under the regulatory control of cytokines induces murine spleen cells to produce NO and that splenic macrophages are the cellular source of the NO production. Therefore, these results may support the view that NO production by LPS-A. actinomycetemcomitans-stimulated macrophages may play a role in the course of periodontal diseases. PMID:16887678

  17. Oxidative and nonoxidative killing of Actinobacillus actinomycetemcomitans by human neutrophils.

    OpenAIRE

    Miyasaki, K T; Wilson, M E; Brunetti, A J; Genco, R J

    1986-01-01

    Actinobacillus actinomycetemcomitans is a facultative gram-negative microorganism which has been implicated as an etiologic agent in localized juvenile periodontitis and in subacute bacterial endocarditis and abscesses. Although resistant to serum bactericidal action and to oxidant injury mediated by superoxide anion (O2-) and hydrogen peroxide (H2O2), this organism is sensitive to killing by the myeloperoxidase-hydrogen peroxide-chloride system (K.T. Miyasaki, M.E. Wilson, and R.J. Genco, In...

  18. Identification of Genes Coding for Exported Proteins of Actinobacillus actinomycetemcomitans

    OpenAIRE

    Mintz, Keith P.; Fives-Taylor, Paula M.

    1999-01-01

    Random fusions of genomic DNA fragments to a partial gene encoding a signal sequence-deficient bacterial alkaline phosphatase were utilized to screen for exported proteins of Actinobacillus actinomycetemcomitans in Escherichia coli. Twenty-four PhoA+ clones were isolated and sequenced. Membrane localization signals in the form of signal sequences were deduced from most of these sequences. Several of the deduced amino acid sequences were found to be homologous to known exported or membrane-ass...

  19. Effect of Ciprofloxacin on Killing of Actinobacillus actinomycetemcomitans by Polymorphonuclear Leukocytes

    OpenAIRE

    Cacchillo, David A.; Walters, John D.

    2002-01-01

    Actinobacillus actinomycetemcomitans, a pathogen associated with aggressive periodontitis, resists phagocytic killing by polymorphonuclear leukocytes (PMNs). It is susceptible to ciprofloxacin, which PMNs actively accumulate. This study tested the hypothesis that ciprofloxacin-loaded PMNs are more effective at killing A. actinomycetemcomitans than control PMNs. Isolated human PMNs were loaded by brief incubation with 0.5 μg of ciprofloxacin/ml. Opsonized bacteria (ATCC 43718) were incubated a...

  20. Detection of highly and minimally leukotoxic Actinobacillus actinomycetemcomitans strains in patients with periodontal disease

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    Cortelli Sheila Cavalca

    2003-01-01

    Full Text Available This study examined the prevalence of highly and minimally leukotoxic Actinobacillus actinomycetemcomitans in patients with periodontal disease. Pooled subgingival plaque samples from 136 patients with some form of periodontal disease were examined. Subjects were between 14 and 76 years of age. Clinical examinations included periodontal pocket depth (PD, plaque index (PI and bleeding index (BI. The obtained plaque samples were examined for the presence of highly or minimally leukotoxic A. actinomycetemcomitans strains by the polymerase chain reaction (PCR. Chi-square and logistic regression were performed to evaluate the results. Forty-seven subjects were diagnosed with gingivitis, 70 with chronic periodontitis and 19 with aggressive periodontitis. According to chi-square there was no significant correlation detected between PD (chi2 = 0.73, PI (chi2 = 0.35, BI (chi2 = 0.09 and the presence of the highly leukotoxic A. actinomycetemcomitans. The highly leukotoxic A. actinomycetemcomitans strains were correlated with subjects that were 28 years of age and younger (chi2 = 7.41. There was a significant correlation between highly leukotoxic A. actinomycetemcomitans and aggressive periodontitis (chi2 = 22.06. This study of a Brazilian cohort confirms the strong association between highly leukotoxic A. actinomycetemcomitans strains and the presence of aggressive periodontitis.

  1. . and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates

    DEFF Research Database (Denmark)

    Nørskov-Lauritsen, N.; Kilian, Mogens

    2006-01-01

    The aim of this study was to reinvestigate the relationships and the generic affiliations of the species Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis. The nicotinamide phosphoribosyltransferase gene (nadV) conferring V factor......-independent growth was identified in Haemophilus aphrophilus. The gene encodes a polypeptide of 462 amino acids that shows 74.5 % amino acid sequence identity to the corresponding enzyme from Actinobacillus actinomycetemcomitans. Ten isolates of Haemophilus paraphrophilus all carried a nadV pseudogene. DNA from...... Haemophilus aphrophilus was able to transform Haemophilus paraphrophilus into the NAD-independent phenotype. The transformants carried a full-length nadV inserted in the former locus of the pseudogene. The DNA-DNA relatedness between the type strains of Haemophilus aphrophilus and Haemophilus paraphrophilus...

  2. Antimicrobial effect of chlorine dioxide on Actinobacillus actinomycetemcomitans in diabetes mellitus rats treated with insulin

    Directory of Open Access Journals (Sweden)

    Tantin Ermawati

    2012-03-01

    Full Text Available Background: Periodontitis is a chronic inflammatory disease of periodontal tissues. Etiology of periodontal disease includes Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans which is the most predominant disease-causing bacteria found in the gingival sulcus. Periodontitis can be exacerbated by the systemic disease, such as diabetes mellitus considered as a metabolic disease characterized by hyperglycemia due to insulin deficiency. Treatment of periodontitis is then required in patients with type I diabetes to avoid radical reaction that can not only cause bleeding, but can also prevent infection, as a result, topical antimicrobial therapy and blood glucose control are required. Topical antimicrobial chlorine dioxide is a disinfectant that is effective in killing A. actinomycetemcomitans. Purpose: This study is aimed to determine the effects of topical antimicrobial chlorine dioxide gel or rinse on the number of A. actinomycetemcomitans in DM rats treated with insulin. Methods: 20 three month old male Wistar rats with weight of 170–200 grams were divided into four groups. First, periodontitis and DM were manipulated into all groups through aloksan injection with dose of 170 mg/kg. Those rats in group I were treated with insulin and chlorine dioxide gel, those in group II were treated with insulin and chlorine dioxide rinse, those in group III were treated with insulin only, and those in group IV were without treatment. In the third and seventh weeks, the number of A. actinomycetemcomitans was measured. The data was tested by using One-Way ANOVA test followed by LSD test. Results: The study showed that chlorine dioxide gel has a greater ability in reducing the number of A. actinomycetemcomitans than chlorine dioxide rinse although both are antimicrobials. Conclusion: It can be concluded that the use of chlorine dioxide gel can more effective to decrease the number of A. actinomycetemcomitans than chlorine dioxide rinse in DM rats

  3. Actinobacillus Actinomycetemcomitans y Porphyromonas Gingivales como principales patógenos periodontales

    Directory of Open Access Journals (Sweden)

    A Bascones

    2000-09-01

    Full Text Available Entre las bacterias relacionadas con la enfermedad periodontal, existen dos especies más claramente asociadas a esta enfermedad: Actinobacillus actinomycetemcomitans y Porphyromonas gingivalis. Este trabajo es una revisión bibliográfica sobre estos dos patógenos periodontales, mostrando su origen, prevalencia, distribución, transmisión y respuesta al tratamiento periodontal.Among the bacteria related to periodontal disease, there are two species clearly associated to this disease: Actinobacillus actinomycetemcomitans and Porphyromonas gingiva lis. This paper presents a review of the literature regarding this two periodontal pathogens, and showing their origin, prevalence, distribution, transmission and response to periodontal treatment.

  4. Early-onset periodontitis in Morocco is associated with the highly leukotoxic clone of Actinobacillus actinomycetemcomitans

    DEFF Research Database (Denmark)

    Haubek, Dorte; Ennibi, O.-K.; Poulsen, Knud;

    2001-01-01

    A particular clone (JP2) of Actinobacillus actinomycetemcomitans with increased leukotoxin production has been isolated from individuals with early-onset periodontitis (EOP). The aim of this study was to determine the frequency of carriers of this clone and its association with EOP in Moroccan...

  5. Differences in iron acquisition from human haemoglobin among strains of Actinobacillus actinomycetemcomitans

    DEFF Research Database (Denmark)

    Hayashida, H.; Poulsen, Knud; Kilian, Mogens

    2002-01-01

    . actinomycetemcomitans strains examined harboured a single genomic sequence with homology to the hgpA gene encoding haemoglobin-binding protein A in Haemophilus influenzae. However, in all three strains belonging to the JP2 clone and in one serotype e strain hgpA was a pseudogene. Seven other strains possessed...

  6. Pyogenic Ventriculitis Complicating Aggregatibacter aphrophilus Infective Endocarditis: A Case Report and Literature Review

    Directory of Open Access Journals (Sweden)

    Gordon W Jung

    2009-01-01

    Full Text Available Pyogenic ventriculitis (PV is an uncommon, but frequently fatal infection that results from inflammation of the ventricular ependymal lining associated with a purulent ventricular system. PV has been rarely reported as a secondary complication of infective endocarditis. Prompt diagnosis and treatment with appropriate culture-directed antibiotics with adequate central nervous system penetration is crucial when managing patients who are suspected of having PV. The present study reports on a fatal case of a previously well 42-year-old alcoholic woman with infective endocarditis caused by Aggregatibacter aphrophilus, with secondary brain abscess and spontaneous rupture into the ventricles causing PV.

  7. Actinobacillus Actinomycetemcomitans y Porphyromonas Gingivales como principales patógenos periodontales

    OpenAIRE

    A. Bascones; A Caballeros

    2000-01-01

    Entre las bacterias relacionadas con la enfermedad periodontal, existen dos especies más claramente asociadas a esta enfermedad: Actinobacillus actinomycetemcomitans y Porphyromonas gingivalis. Este trabajo es una revisión bibliográfica sobre estos dos patógenos periodontales, mostrando su origen, prevalencia, distribución, transmisión y respuesta al tratamiento periodontal.Among the bacteria related to periodontal disease, there are two species clearly associated to this disease: Actinobacil...

  8. In vitro activity of antibiotics alone and in combination against Actinobacillus actinomycetemcomitans.

    Science.gov (United States)

    Yogev, R; Shulman, D; Shulman, S T; Glogowski, W G

    1986-01-01

    The MICs for 90% of the organisms tested (MIC90S) of 11 antibiotics against 24 clinical isolates of Actinobacillus actinomycetemcomitans were determined by the MIC 2000 system. The lowest MIC90S (16 micrograms/ml) were observed with ceftriaxone and rifampin. The next lowest MIC90S were found with cephapirin, tetracycline, and chloramphenicol (3.12 micrograms/ml). The MIC90S of penicillin, ampicillin, ticarcillin, piperacillin, and amikacin were each greater than or equal to 12.5 micrograms/ml. Antibiotic synergy was studied by the killing curve method and was defined as a greater than or equal to 2 log10 reduction in CFU when two antibiotics were used in combination at one-fourth the MBC for each compared with the effect of each antibiotic alone at one-half the MBC. Synergism between rifampin and penicillin, cephapirin, or ceftriaxone was tested for with 12 A. actinomycetemcomitans strains. In 7 of 37 instances, synergism was demonstrated for the combinations rifampin plus ceftriaxone (n = 3) or rifampin plus penicillin (n = 4); in 9 instances, an additive effect was noted, and impaired killing with drug combinations compared with the effect of a single antibiotic was suggested in 4 strains. The majority of strains were indifferent to the combinations. Similarly, variable results were observed when the combination of trimethoprim and cephapirin was tested against eight A. actinomycetemcomitans strains. Our data suggest that rifampin and cephapirin are the most active of the 11 antibiotics studied against A. actinomycetemcomitans. In addition, in vitro synergism between rifampin and other antibiotics or between trimethoprim and cephapirin was not consistently demonstrable.

  9. Recombinant Actinobacillus actinomycetemcomitans Cytolethal Distending Toxin Proteins Are Required To Interact To Inhibit Human Cell Cycle Progression and To Stimulate Human Leukocyte Cytokine Synthesis

    OpenAIRE

    Akifusa, Sumio; Poole, Stephen; Lewthwaite, Jo; Henderson, Brian; Nair, Sean P

    2001-01-01

    It has recently been discovered that Actinobacillus actinomycetemcomitans, an oral bacterium causing periodontitis, produces cytolethal distending toxin (CDT), a cell cycle-modulating toxin that has three protein subunits: CdtA, CdtB, and CdtC. In this study, we have cloned and expressed each toxin gene from A. actinomycetemcomitans in Escherichia coli and purified the recombinant Cdt proteins to homogeneity. Individual Cdt proteins failed to induce cell cycle arrest of the human epithelial c...

  10. NCBI nr-aa BLAST: CBRC-MLUC-01-1054 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MLUC-01-1054 ref|YP_003255210.1| TrkG protein [Aggregatibacter actinomycetemcomita...ns D11S-1] gb|ACX81991.1| TrkG protein [Aggregatibacter actinomycetemcomitans D11S-1] YP_003255210.1 0.64 23% ...

  11. Antioxidant effect of minocycline in gingival epithelium induced by Actinobacillus actinomycetemcomitans serotype B toxin

    Directory of Open Access Journals (Sweden)

    Ernie Maduratna Setiawati

    2009-03-01

    Full Text Available Background: Actinobacillus actinomycetemcomitans (Aa serotype B has been associated with aggressive periodontitis. Gingival epithelial cell is exquisitely sensitive to the toxin and may lead to the epithel protective barrier disruption. Experimental models show that minocycline is not related to it’s antimicrobial effect and protection against neuron cell apoptosis of a number experimental models of brain injury and Parkinson’s disease. Purpose: This study, examined antioxidant effect of minocycline to inhibit apoptosis of gingival epithelium induced crude toxin bacteria Aa serotype B in mice. Methods: Thirty adult mice strain Swiss Webster (balb C were divided randomly into three groups: control group (group A, toxin group (group B and toxin and minocycline group (group C. The mice were taken at 24 hours after application, and then the tissue sections of gingival epithelium were stained with tunnel assay and immunohistochemistry. Result: Treatment with these toxin induced apoptosis of gingival epithelium and was associated with DNA fragmentation and reduced gluthatione (GSH. Minocycline 100 nM significantly increased GSH and reduced apoptosis (p < 0.05. Minocycline provides antioxidant effect against citotoxicity of bacteria Aa serotipe B. Conclusion: Nanomolar concentration of minocycline potential as new therapeutic agent to prevent progressivity of aggressiveness of periodontitis.

  12. Site-specific subgingival colonization by Actinobacillus actinomycetemcomitans in orthodontic patients.

    Science.gov (United States)

    Paolantonio, M; Festa, F; di Placido, G; D'Attilio, M; Catamo, G; Piccolomini, R

    1999-04-01

    A high prevalence of Actinobacillus actinomycetemcomitans (Aa) in subgingival plaque in patients for orthodontia already has been observed. The present study had the following aims: 1) to ascertain a direct relationship between the orthodontic appliance placement and the subgingival colonization by Aa, and 2) to determine whether the Aa growth specifically occurred on teeth with braces attached or whether the presence of orthodontic appliances could also cause the isolation of Aa in teeth free from therapeutic appliances. Twenty-four young systemically and periodontally healthy subjects with malaligned and crowded teeth in the anterior sextants of both dental arches participated in this study. After 1 session of ultrasonic scaling with oral hygiene instructions during the first experimental session, the mesiobuccal sites of the first molars and the distobuccal sites of the lateral incisors in both dental arches in each participant were subjected to clinical and microbiologic examination for the recovery of Aa. Clinical examination consisted of recording the presence of plaque and the examination of gingival bleeding on probing and probing depth. Microbiologic sampling was obtained with the insertion of 3 sterile paper points at the deepest part of each gingival sulcus. Altogether, 192 periodontal sites were examined. After the examinations, the patients received fixed orthodontic appliances in only 1 dental arch (test sites) and the other one was left free from appliances (control sites). Clinical examination and microbiologic sampling were repeated in the same experimental test and control sites after 4, 8, and 12 weeks. At the 12-week session, the orthodontic appliance was removed from the test arch, and, 4 weeks later, a further clinical and microbiologic examination was performed. The results showed that, during the period with orthodontic appliances, the presence of plaque scores and the gingival bleeding on probing scores were increased significantly and that

  13. 关于Diophantine方程x2+y4=z5%On the Diophantine Equation x2+y4=z5

    Institute of Scientific and Technical Information of China (English)

    乐茂华

    2009-01-01

    运用无穷递降法证明了:方程X4-10X2Y2+5Y4=Z2和X4-50X2Y2+125Y4=Z2都没有适合gcd(X,Y)=1以及2|XY的正整数解(X,Y,Z).由此推知:方程x2+y4=z5没有适合gcd(x, y)=1的正整数解(x,y,z),上述结果解决了广义Fermat猜想的一个特殊情况.

  14. Efectos de un gel de tetraciclina al 5% sobre los niveles de P. gingivalis, P. intermedia y A. actinomycetemcomitans

    Directory of Open Access Journals (Sweden)

    F. Gallardo

    2002-04-01

    Full Text Available El objetivo de la presente investigación fue estudiar los efectos de un gel de tetraciclina al 5% sobre los niveles de 3 microorganismos asociados al desarrollo de la periodontitis rápidamente progresiva (PRP. En un total de 20 pacientes con PRP se seleccionaron 5 dientes por paciente con bolsas periodontales > a 5 mm. con sangrado al sondaje periodontal en los cuales se efectuaron los siguientes tratamientos: 1. Control. 2. Aplicación de un gel de tetraciclina al 5% (T. 3. Placebo (Pl. 4. Raspado y alisado radicular (RA. 5. T + RA .De forma previa, en cada sitio seleccionado, se tornaron muestras de placa subgingival con un cono de papel estéril para detectar y cuantificar la presencia de P. gingivalis , P. intermedia y A. actinomycetemcomitans, mediante el uso de sondas DNA (OMNIGENE, U.S.A..Se dieron instrucciones de higiene oral, y se efectuó un nuevo control microbiológico a los 60 días El análisis estadístico de los resultados demostró lo siguiente: 1. Ninguno de los tratamientos redujo significativamente los niveles de A. actinomycetemcomitans. 2. Se detectó una reducción significativa de P. gingivalis en los sitios tratados con (R.A. (p The purpose of this investigation was to study the effects of local delivery of a tetracycline 5% gel on the levels of 3 bacteria associated to development of rapidly progressive periodontitis. In a sample of 20 patients, five teeth were selected from each patient with periodontal pockets > 5 mm and bleeding upon probing. One of the following treatments were done at each selected site: 1. Control (no treatment. 2. Local delivery of a 5% tetracycline gel. 3. Placebo gel. 4. Scaling and root planing. 5. Scaling and root planing + local application of tetracycline gel. Previously, at each selected site samples of subgingival plaque were taken with sterile paper points in order to detecte and quantify the presence of P.gingivalis, P.intermedia and A. actinomycetemcomitans, by using DNA probe

  15. Efectos de un gel de tetraciclina al 5% sobre los niveles de P. gingivalis, P. intermedia y A. actinomycetemcomitans

    Directory of Open Access Journals (Sweden)

    F. Gallardo

    2002-04-01

    Full Text Available El objetivo de la presente investigación fue estudiar los efectos de un gel de tetraciclina al 5% sobre los niveles de 3 microorganismos asociados al desarrollo de la periodontitis rápidamente progresiva (PRP. En un total de 20 pacientes con PRP se seleccionaron 5 dientes por paciente con bolsas periodontales > a 5 mm. con sangrado al sondaje periodontal en los cuales se efectuaron los siguientes tratamientos: 1. Control. 2. Aplicación de un gel de tetraciclina al 5% (T. 3. Placebo (Pl. 4. Raspado y alisado radicular (RA. 5. T + RA .De forma previa, en cada sitio seleccionado, se tornaron muestras de placa subgingival con un cono de papel estéril para detectar y cuantificar la presencia de P. gingivalis , P. intermedia y A. actinomycetemcomitans, mediante el uso de sondas DNA (OMNIGENE, U.S.A..Se dieron instrucciones de higiene oral, y se efectuó un nuevo control microbiológico a los 60 días El análisis estadístico de los resultados demostró lo siguiente: 1. Ninguno de los tratamientos redujo significativamente los niveles de A. actinomycetemcomitans. 2. Se detectó una reducción significativa de P. gingivalis en los sitios tratados con (R.A. (p <0.02 o cuando este ultimo tratamiento se asoció a (T. (p <0.05 3. Todos los tratamientos redujeron significativamente los niveles de P. intermedia. Aunque las tetraciclinas incluyen en su espectro de acción a las bacterias estudiadas, y alcanzan altas concentración después de su aplicación en los sitios respectivos, los reducidos efectos microbiológicos encontrados podrían explicarse por su rápida remoción por el fluido gingival desde su sitio de aplicación en las localizaciones estudiadas.

  16. Association between infection of different strains of Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in subgingival plaque and clinical parameters in chronic periodontitis

    Institute of Scientific and Technical Information of China (English)

    WU Yan-min; YAN Jie; CHEN Li-li; GU Zhi-yuan

    2007-01-01

    Objective: The aim of this study was to investigate subgingival infection frequencies ofPorphyromonas gtngivalis and Actinobacillus actinomycetemcomitans strains with genetic variation in Chinese chronic periodontitis (CP) patients and to evaluate its correlation with clinical parameters. Methods: Two multiplex polymerase chain reaction (PCR) assays were developed to detect the 16SrDNA, collagenase (prtC) and fimbria (fimA) genes of P. gingivalis and the 16SrDNA, leukotoxin (lktA) and fimbria-associated protein (lap) genes ofA. actinomycetemcomitans in 60 sulcus samples from 30 periodontal healthy subjects and in 122 subgingival plaque samples from 61 patients with CP. The PCR products were further T-A cloned and sent for nucleotide sequence analysis. Results: The 16SrDNA, prtC andfimA genes ofP. gingivalis were detected in 92.6%, 85.2% and 80.3% of the subgingival plaque samples respectively, while the 16SrDNA, lktA andfap genes ofA. actinomycetemcomitans were in 84.4%,75.4% and 50.0% respectively. Nucleotide sequence analysis showed 98.62%~100% homology of the PCR products in these genes with the reported sequences. P. gingivalis strains with prtC+/fimA+ and A. actinomycetemcomitans with lktA+ were predominant in deep pockets (>6 mm) or in sites with attachment loss ≥5 mm than in shallow pockets (3~4 mm) or in sites with attachment loss ≤2 mm (P<0.05). P. gingivalis strains with prtC+/fimA+ also showed higher frequency in gingival index (GI)=3than in GI= 1 group (P<0.05). Conclusion: Infection of P. gingivalis with prtC+/fimA+ and A. actinomycetemcomitans with lktA+correlates with periodontal destruction of CP in Chinese. Nonetheless P. gingivalis fimA, prtC genes and A. actinomycetemcomitans IktA gene are closely associated with periodontal destruction, while A. actinomycetemcomitansfap gene is not.

  17. Study on transfection method to MLO-Y4 cells%小鼠骨样细胞MLO-Y4转染方法的研究

    Institute of Scientific and Technical Information of China (English)

    安龙; 续惠云; 瓮媛媛; 商澎

    2010-01-01

    为了建立质粒转染小鼠骨样细胞MLO-Y4的方法,分别采用阳离子脂质体法和电转染法将增强型绿色荧光蛋白(EGFP)质粒pEGFP-C1转染小鼠骨样细胞MLO-Y4,正常培养48h后检测并统计转染率和死亡率.结果显示,脂质体法转染,当质粒与脂质体比例为1∶4时,转染效率可达到(36.8 ±3.7)%,细胞死亡率为(18.4 ±1.9)%;电转染法转染,脉冲电压240 V,脉冲时间300μs,脉冲次数3次时,转染率最高,可达到(23.8 ±2.3)%,细胞死亡率为(14.1 ±1.1)%.而后MTT实验显示脂质体转染法相对于电转柒法对MLO-Y4细胞的增殖有一定的抑制作用,但对后续实验研究影响不大.脂质体转染法转染小鼠骨样细胞MLO-Y4优于电转染法.

  18. The bactericidal effect of a Genius (R) Nd : YAG laser

    NARCIS (Netherlands)

    Kranendonk, A.A.; Reijden, W.A. van der; Winkelhoff, A.J. van; Weijden, G.A. van der

    2010-01-01

    PURPOSE: To evaluate the 'in vitro' bactericidal effect of the Nd:YAG laser (Genius, MØlsgaard Dental, Copenhagen, Denmark) on six periodontal pathogens. METHODS: Suspensions of six different periodontal pathogens (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedi

  19. Association between coinfection of Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Treponema denticola and periodontal tissue destruction in chronic periodontitis

    Institute of Scientific and Technical Information of China (English)

    CHEN Li-li; WU Yan-min; YAN Jie; SUN Wei-lian; SUN Yu-zheng; David Ojcius

    2005-01-01

    Background The association between the infection of Porphyromonas gingivalis, Actinobacillus actinomy-cetemcomitans and Treponema denticola in chronic periodontitis (CP) and the severity of periodontal disease remains to be elucidated. The aim of this study was to investigate the subgingival infection frequencies of three periodontopathic bacteria in Chinese CP patients and to evaluate the correlations between infection by these bacteria and periodontal destruction.Methods A multiple PCR assay using primers derived from 16SrDNA genes of P. gingivalis, A. actinomy-cetemitans and T. denticola was established to measure simultaneously the presence of the three microbes in 162 subgingival samples from 81 Chinese CP patients. Results The positive rates of P. gingivalis, A. actinomycetemitans and T. denticola in the subgingival samples were 84.6%, 83.3% and 88.3%, respectively. Of the subgingival samples, 68% revealed the coinfection of all the three microbes. The infection rates with P. gingivalis, A. actinomycetemitans or T. denticola alone was 5.9% (1/17), 17.6% (3/17) and 76.5% (13/17), respectively. A close association was present between the A. actinomycetemitans infection and gingival index (GI) (P0.05). P. gingivalis and A. actinomycetemitans were more frequently detectable in middle and deep pockets than in shallow ones (P<0.01), while T. denticola was found remarkably often in deep pockets (P<0.05). The coinfection rate of the three microbes was significantly higher in sites with severe periodontitis than in those with mild periodontitis (P<0.01). Conclusions The multiple PCR established in this study can be used as a sensitive and specific method to simultaneously detect all three microbes in subgingival samples. A. actinomycetemitans infection may be associated with CP and play an important role in the periodontal tissue destruction. The coinfection of P. gingivalis, A. actinomycetemitans and T. denticola can cause more serious periodontal destruction than

  20. Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes.

    Science.gov (United States)

    Juffer, Petra; Jaspers, Richard T; Lips, Paul; Bakker, Astrid D; Klein-Nulend, Jenneke

    2012-02-15

    Lack of physical activity results in muscle atrophy and bone loss, which can be counteracted by mechanical loading. Similar molecular signaling pathways are involved in the adaptation of muscle and bone mass to mechanical loading. Whether anabolic and metabolic factors regulating muscle mass, i.e., insulin-like growth factor-I isoforms (IGF-I Ea), mechano growth factor (MGF), myostatin, vascular endothelial growth factor (VEGF), or hepatocyte growth factor (HGF), are also produced by osteocytes in bone in response to mechanical loading is largely unknown. Therefore, we investigated whether mechanical loading by pulsating fluid flow (PFF) modulates the mRNA and/or protein levels of muscle anabolic and metabolic factors in MLO-Y4 osteocytes. Unloaded MLO-Y4 osteocytes expressed mRNA of VEGF, HGF, IGF-I Ea, and MGF, but not myostatin. PFF increased mRNA levels of IGF-I Ea (2.1-fold) and MGF (2.0-fold) at a peak shear stress rate of 44Pa/s, but not at 22Pa/s. PFF at 22 Pa/s increased VEGF mRNA levels (1.8- to 2.5-fold) and VEGF protein release (2.0- to 2.9-fold). Inhibition of nitric oxide production decreased (2.0-fold) PFF-induced VEGF protein release. PFF at 22 Pa/s decreased HGF mRNA levels (1.5-fold) but increased HGF protein release (2.3-fold). PFF-induced HGF protein release was nitric oxide dependent. Our data show that mechanically loaded MLO-Y4 osteocytes differentially express anabolic and metabolic factors involved in the adaptive response of muscle to mechanical loading (i.e., IGF-I Ea, MGF, VEGF, and HGF). Similarly to muscle fibers, mechanical loading enhanced expression levels of these growth factors in MLO-Y4 osteocytes. Although in MLO-Y4 osteocytes expression levels of IGF-I Ea and MGF of myostatin were very low or absent, it is known that the activity of osteoblasts and osteoclasts is strongly affected by them. The abundant expression levels of these factors in muscle cells, in combination with low expression in MLO-Y4 osteocytes, provide a

  1. Serotonin Transporter and Receptor Expression in Osteocytic MLO-Y4 Cells

    OpenAIRE

    BLIZIOTES, M.; ESHLEMAN, A.; BURT-PICHAT, B.; Zhang, X.-W.; Hashimoto, J; WIREN, K.; C. Chenu

    2006-01-01

    Neurotransmitter regulation of bone metabolism has been a subject of increasing interest and investigation. We reported previously that osteoblastic cells express a functional serotonin (5-HT) signal transduction system, with mechanisms for responding to and regulating uptake of 5-HT. The clonal murine osteocytic cell line, MLO-Y4, demonstrates expression of the serotonin transporter (5-HTT), and the 5-HT1A, and 5-HT2A receptors by real-time RT-PCR and immunoblot analysis. Immunohistochemistr...

  2. Effect of Eu3+ Concentration on Luminescence Studies of Y4Al2O9 Phosphor

    Directory of Open Access Journals (Sweden)

    Vikas Dubey

    2014-01-01

    Full Text Available The present paper reports the effect of europium concentration on photoluminescence (PL and thermoluminescence (TL studies of Eu3+ doped Y4Al2O9 phosphor using inorganic materials like yttrium oxide (Y2O3, aluminium oxide (Al2O3, boric acid (H3BO3 as a flux, and europium oxide (Eu2O3. The sample was prepared by the modified solid state reaction method, which is the most suitable for large-scale production. The prepared phosphor sample was characterized using X-ray diffraction (XRD, field emission gun scanning electron microscopy (FEGSEM, Fourier transform infrared spectroscopy (FTIR, photoluminescence (PL, thermoluminescence (TL, and CIE techniques. The PL emission was observed in the range of 467, 535, 591, 611, 625, and 629 nm for the Y4Al2O9 phosphor doped with Eu3+ (0.1 mol% to 2.5 mol%. Excitation spectrum was found at 237 and 268 nm. Sharp peaks were found around 591, 611, and 625 nm with high intensity. From the XRD data, using Scherer’s formula, the calculated average crystallite size of Eu3+ doped Y4Al2O9 the phosphor is around 55 nm. Thermoluminescence study was carried out for the phosphor with UV irradiation. The present phosphor can act as single host for red light emission in display devices.

  3. Temperature and concentration quenching of Tb3+ emissions in Y4Al2O9 crystals

    International Nuclear Information System (INIS)

    Highlights: ► Spectroscopic properties of Tb3+:Y4Al2O9 crystals are studied. ► Concentration and temperature dependencies of fluorescence are investigated. ► The cross-relaxation transfer rates are experimentally determined. ► Strong influence of cross relaxation process on 5D3 emission quenching is observed. ► Decays are modelled using Inokuti–Hirayama approach. - Abstract: Spectroscopic properties of trivalent terbium (Tb3+) activated Y4Al2O9 (abbreviated YAM) crystals were studied. Concentration and temperature dependent emission spectra and fluorescence dynamics profiles have been investigated in YAM:Tb3+ in order to understand better processes responsible for quenching of the terbium 5D3 and 5D4 emissions. Decays were modelled using Inokuti–Hirayama approach to obtain information on the energy transfer mechanism. The cross-relaxation transfer rates were experimentally determined as a function of temperature and Tb3+ concentration. The investigation revealed strong influence of cross-relaxation process on 5D3 emission quenching. The two different processes responsible for the increase of fluorescence quenching with growing temperature were observed, both related to thermal activation energy. For temperatures above 700 K, the temperature dependence of the emission intensity ratio (5D3/5D4) becomes linear and the decay times are rapidly decreasing monotonously with increasing temperature, what is confirming the potential of Y4Al2O9:Tb3+ material in high temperature luminescence thermometry.

  4. Actividad antiinflamatoria de d-amirona y 4, 7-dimetoxiapigenina aislados de alnus acuminata

    OpenAIRE

    Salama, Ahmed; Avendaño, Inés Yamile

    2009-01-01

    El presente trabajo determinó el efecto antiinflamatorio de d-amirona (olean-13(18)-en-3-ona) y 4',7-dimetoxiapigenina (5-hidroxi-4¢,7-dimetoxiflavona), aislados de Alnus acuminata (Betulaceae), por el método del edema plantar en ratas hembra, en dosis de 30, 60 y 100 mg/kg y de 30, 60 y 80 mg/kg respectivamente. Ambas sustancias mostraron una actividad antiinflamatoria significativa. El efecto más alto de d-amirona se presentó a la primera hora en las tres dosis ensayadas comparable con el e...

  5. Acetylcholine affects osteocytic MLO-Y4 cells via acetylcholine receptors.

    Science.gov (United States)

    Ma, Yuanyuan; Li, Xianxian; Fu, Jing; Li, Yue; Gao, Li; Yang, Ling; Zhang, Ping; Shen, Jiefei; Wang, Hang

    2014-03-25

    The identification of the neuronal control of bone remodeling has become one of the many significant recent advances in bone biology. Cholinergic activity has recently been shown to favor bone mass accrual by complex cellular regulatory networks. Here, we identified the gene expression of the muscarinic and nicotinic acetylcholine receptors (m- and nAChRs) in mice tibia tissue and in osteocytic MLO-Y4 cells. Acetylcholine, which is a classical neurotransmitter and an osteo-neuromediator, not only influences the mRNA expression of the AChR subunits but also significantly induces the proliferation and viability of osteocytes. Moreover, acetylcholine treatment caused the reciprocal regulation of RANKL and OPG mRNA expression, which resulted in a significant increase in the mRNA ratio of RANKL:OPG in osteocytes via acetylcholine receptors. The expression of neuropeptide Y and reelin, which are two neurogenic markers, was also modulated by acetylcholine via m- and nAChRs in MLO-Y4 cells. These results indicated that osteocytic acetylcholine receptors might be a new valuable mediator for cell functions and even for bone remodeling. PMID:24508663

  6. Overexpression, purification, crystallization and preliminary X-ray analysis of CheY4 from Vibrio cholerae O395

    International Nuclear Information System (INIS)

    The chemotaxis response regulator CheY4 from V. cholerae has been cloned, overexpressed, purified and crystallized in monoclinic and hexagonal space groups; the crystals diffracted to 1.67 and 1.9 Å resolution, respectively. Chemotaxis and motility greatly influence the infectivity of Vibrio cholerae, although the role of chemotaxis genes in V. cholerae pathogenesis is poorly understood. In contrast to the single copy of CheY found in Escherichia coli and Salmonella typhimurium, four CheYs (CheY1–CheY4) are present in V. cholerae. While insertional disruption of the cheY4 gene results in decreased motility, insertional duplication of this gene increases motility and causes enhanced expression of the two major virulence genes. Additionally, cheY3/cheY4 influences the activation of the transcription factor NF-κB, which triggers the generation of acute inflammatory responses. V. cholerae CheY4 was cloned, overexpressed and purified by Ni–NTA affinity chromatography followed by gel filtration. Crystals of CheY4 grown in space group C2 diffracted to 1.67 Å resolution, with unit-cell parameters a = 94.4, b = 31.9, c = 32.6 Å, β = 96.5°, whereas crystals grown in space group P3221 diffracted to 1.9 Å resolution, with unit-cell parameters a = b = 56.104, c = 72.283 Å, γ = 120°

  7. Laser site-selective spectroscopy of Eu3+ ions doped Y4Al2O9

    Science.gov (United States)

    Kaczkan, M.; Turczyński, S.; Pawlak, D. A.; Wencka, M.; Malinowski, M.

    2016-08-01

    Eu3+ doped Y4Al2O9 (YAM) crystals were prepared by the micro-pulling down method. Optical-absorption and laser-selective-excitation techniques along with the luminescence decays have been used to reveal that Eu3+ ions in YAM occupy three distinct sites, which were characterized and discussed. The Stark energy levels of Eu3+ at three different sites in YAM were assigned from selectively excited emission spectra at 10 K. The intensity ratio of forced electric dipole (5D0 → 7F2) and magnetic dipole (5D0 → 7F1) transitions was discussed in order to obtain information about the degree of asymmetry of the luminescent centers. These results were confirmed by the luminescence lifetime measurements. The temperature dependent photo-luminescence spectra indicated that there is no energy transfer between different sites in the 10-300 K range.

  8. Corticosterone regulates the expression of neuropeptide Y and reelin in MLO-Y4 cells.

    Science.gov (United States)

    Ma, Yuanyuan; Wu, Xiangnan; Li, Xianxian; Fu, Jing; Shen, Jiefei; Li, Xiaoyu; Wang, Hang

    2012-06-01

    Osteocytes that have a dendritic appearance are widely believed to form a complex cellular network system and play crucial roles in mechanotransduction as a principal bone mechanosensor, which is the basis of their neuronallike biology, as previously reported. Neuropeptide Y (NPY) and reelin mRNA, which are brain-specific neurogenic markers, have been identified in osteocytes. However, changes in the production of NPY and reelin in response to specific biochemical stimulation are unknown. In this study, we investigated the in vitro effect of corticosterone, one of the endogenous glucocorticoids, on the expression of NPY and reelin in the MLO-Y4 osteocyte cell line. Cells were treated with corticosterone at different concentrations (10(-9) M-10(-5) M) for 1, 3, 6, 12 and 24 h. As revealed, corticosterone reduced the MLO-Y4 cell viability and proliferation in a dose- and time-dependent manner based on an MTT assay and a Vi-CELL analyzer. The cells were then incubated with corticosterone (10(-6) μM), and the NPY and reelin expression levels were detected at 1, 3, 6, 12 and 24 h using real-time PCR and Western blot analysis. These results demonstrated that at the gene and the protein levels, corticosterone significantly upregulated the NPY and reelin expression in a time-dependent manner. The application of a glucocorticoid receptor antagonist, RU486, reversed the reduced cell viability and the increased expression of NPY and reelin that were caused by corticosterone. To the best of our knowledge, this is the first report to verify that corticosterone regulates the NPY and reelin expression in osteocytes. PMID:22610366

  9. Study of hadronic transitions between Y states and observation of Y(4S)-> eta Y(1S) decay

    CERN Document Server

    Aubert, B; Karyotakis, Yu; Lees, J P; Poireau, V; Prencipe, E; Prudent, X; Tisserand, V; Garra Tico, J; Graugès-Pous, E; López, L; Palano, A; Pappagallo, M; Eigen, G; Stugu, B; Sun, L; Abrams, G S; Battaglia, M; Brown, D N; Cahn, R N; Jacobsen, R G; Kerth, L T; Kolomensky, Yu G; Kukartsev, G; Lynch, G; Osipenkov, I L; Ronan, M T; Tackmann, K; Tanabé, T; Hawkes, C M; Soni, N; Watson, A T; Koch, H; Schröder, T; Walker, D; Asgeirsson, D J; Fulsom, B G; Hearty, C; Mattison, T S; McKenna, J A; Barrett, M; Khan, A; Teodorescu, L; Blinov, V E; Bukin, A D; Buzykaev, A R; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, K Yu; Bondioli, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M; Martin, E C; Stoker, D P; Abachi, S; Buchanan, C; Gary, J W; Liu, F; Long, O; Shen, B C; Vitug, G M; Yasin, Z; Zhang, L; Sharma, V; Campagnari, C; Hong, T M; Kovalskyi, D; Mazur, M A; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Schalk, T; Schumm, B A; Seiden, A; Wang, L; Wilson, M G; Winstrom, L O; Cheng, C H; Doll, D A; Echenard, B; Fang, F; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Andreassen, R; Mancinelli, G; Meadows, B T; Mishra, K; Sokoloff, M D; Bloom, P C; Ford, W T; Gaz, A; Hirschauer, J F; Kreisel, A; Nagel, M; Nauenberg, U; Smith, J G; Ulmer, K A; Wagner, S R; Ayad, R; Soffer, A; Toki, W H; Wilson, R J; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Karbach, M; Merkel, J; Petzold, A; Spaan, B; Wacker, K; Kobel, M J; Mader, W F; Nogowski, R; Schubert, K R; Schwierz, R; Sundermann, J E; Volk, A; Bernard, D; Bonneaud, G R; Latour, E; Thiebaux, C; Verderi, M; Clark, P J; Gradl, W; Playfer, S; Watson, J E; Andreotti, M; Bettonia, D; Bozzia, C; Calabrese, R; Cecchi, A; Cibinetto, G; Franchini, P; Luppiab, E; Negrini, M; Petrella, A; Piemontesea, L; Santoro, V; Baldini-Ferroli, R; Calcaterra, A; De Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzoa, A; Contri, R; Lo Vetere, M; Macria, M M; Monge, M R; Passaggioa, S; Patrignani, C; Robuttia, E; Santroni, A; Tosi, S; Chaisanguanthum, K S; Morii, M; Marks, J; Schenk, S; Uwer, U; Klose, V; Lacker, H M; Bard, D J; Dauncey, P D; Nash, J A; Panduro-Vazquez, W; Tibbetts, M; Behera, P K; Chai, X; Charles, M J; Mallik, U; Cochran, J; Crawley, H B; Dong, L; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Gao, Y Y; Gritsan, A V; Guo, Z J; Lae, C K; Denig, A G; Fritsch, M; Schott, G; Arnaud, N; Bquilleux, J; D'Orazio, A; Davier, M; Firminoda Costa, J; Grosdidier, e G; Höcker, A; Lepeltier, V; Le Diberder, F; Lutz, A M; Pruvot, S; Roudeau, P; Schune, M H; Serrano, J; Sordini, V; Stocchi, A; Wormser, G; Lange, D J; Wright, D M; Bingham, I; Burke, J P; Chavez, C A; Fry, J R; Gabathuler, E; Gamet, R; Hutchcroft, D E; Payne, D J; Touramanis, C; Bevan, A J; Clarke, C K; George, K A; Di Lodovico, F; Sacco, R; Sigamani, M; Cowan, G; Flächer, H U; Hopkins, D A; Paramesvaran, S; Salvatore, F; Wren, A C; Brown, D N; Davis, C L; Alwyn, K E; Bailey, D S; Barlow, R J; Chia, Y M; Edgar, C L; Lafferty, G D; West, T J; Yi, J I; Anderson, J; Chen, C; Jawahery, A; Roberts, D A; Simi, G; Tuggle, J M; Dallapiccola, C; Li, X; Salvati, E; Saremi, S; Cowan, R; Dujmic, D; Fisher, P H; Koeneke, K; Sciolla, G; Spitznagel, M; Taylor, F; Yamamoto, R K; Zhao, M; Patel, P M; Robertson, S H; Lazzaro, A; Lombardoa, V; Palombo, F; Bauer, J M; Cremaldi, L; Eschenburg, V; Godang, R; Kroeger, R; Sanders, D A; Summers, D J; Zhao, H W; Simard, M; Taras, P; Viaud, F B; Nicholson, H; De Nardo, Gallieno; Listaa, L; Monorchio, D; Onorato, G; Sciacca, C; Raven, G; Snoek, H L; Jessop, C P; Knoepfel, K J; LoSecco, J M; Wang, W F; Benelli, G; Corwin, L A; Honscheid, K; Kagan, H; Kass, R; Morris, J P; Rahimi, A M; Regensburger, J J; Sekula, S J; Wong, Q K; Blount, N L; Brau, J E; Frey, R; Igonkina, O; Kolb, J A; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Castelliab, G; Gagliardi, N; Margoni, M; Morandina, M; Posoccoa, M; Rotondoa, M; Simonettoab, F; Stroiliab, R; Vociab, C; Del Amo-Sánchez, P; Ben-Haim, E; Briand, H; Calderini, G; Chauveau, J; David, P; Del Buono, L; Hamon, O; Leruste, P; Ocariz, J; Pérez, A; Prendki, J; Gladney, L; Biasini, M; Covarelli, R; Manoniab, E; Angeliniab, C; Batignaniab, G; Bettariniab, S; Carpinelli, M; Cervelliab, A; Fortiab, F; Giorgi, M A; Lusianiac, A; Marchiori, G; Morganti, M; Neri, N; Paoloni, E; Rizzo, G; Walsha, J J; Biesiada, J; Lopes-Pegna, D; Lü, C; Olsen, J; Smith, A J S; Telnov, A V; Anullia, F; Baracchini, E; Cavotoa, G; del Reab, D; Di Marcoab, E; Facciniab, R; Ferrarottoa, F; Ferroniab, F; Gasperoab, M; Jacksona, P D; Li Gioia, L; Mazzonia, M A; Morgantia, S; Pireddaa, G; Polciab, F; Rengaab, F; Voenaa, C; Ebert, M; Hartmann, T; Schröder, H

    2008-01-01

    We present a study of hadronic transitions between Y(mS) (m=4,3,2) and Y(nS) (n=2,1) resonances based on 347.5\\invfb of data taken with the BABAR detector at the PEP-II storage rings. We report the first observation of Y(4S)-> eta Y(1S) decay with a branching fraction BR((Y(4S)->eta Y(1S))=(1.96+-0.06_{stat} +-0.09_{syst}) x 10^{-4} and measure the ratio of partial widths Gamma(Y(4S)->etaY(1S))/Gamma(Y(4S)->pi+pi-Y(1S))=2.41+- 0.40_{stat}+- 0.12_{syst}. We set 90% CL upper limits on the ratios Gamma(Y(2S)->etaY(1S))/Gamma(Y(2S)->pi+pi-Y(1S))etaY(1S))/Gamma(Y(3S)->pi+pi-Y(1S))pi+pi-Y(2S))/Gamma(Y(4S)->pi+pi-Y(1S))=1.16+- 0.16_{stat}+- 0.14_{syst} and Gamma(Y(3S)->pi+pi-Y(2S))/Gamma(Y(3S)->pi+pi-Y(1S))=0.577+- 0.026_{stat}+- 0.060_{syst}.

  10. Optically Pure, Structural, and Fluorescent Analogues of a Dimeric Y4 Receptor Agonist Derived by an Olefin Metathesis Approach.

    Science.gov (United States)

    Liu, Mengjie; Mountford, Simon J; Richardson, Rachel R; Groenen, Marleen; Holliday, Nicholas D; Thompson, Philip E

    2016-07-14

    The dimeric peptide 1 (BVD-74D, as a diastereomeric mixture) is a potent and selective neuropeptide Y Y4 receptor agonist. It represents a valuable candidate in developing traceable ligands for pharmacological studies of Y4 receptors and as a lead compound for antiobesity drugs. Its optically pure stereoisomers along with analogues and fluorescently labeled variants were prepared by exploiting alkene metathesis reactions. The (2R,7R)-diaminosuberoyl containing peptide, (R,R)-1, had markedly higher affinity and agonist efficacy than its (S,S)-counterpart. Furthermore, the sulfo-Cy5 labeled (R,R)-14 retained high agonist potency as a novel fluorescent ligand for imaging Y4 receptors. PMID:27295337

  11. Optically Pure, Structural, and Fluorescent Analogues of a Dimeric Y4 Receptor Agonist Derived by an Olefin Metathesis Approach.

    Science.gov (United States)

    Liu, Mengjie; Mountford, Simon J; Richardson, Rachel R; Groenen, Marleen; Holliday, Nicholas D; Thompson, Philip E

    2016-07-14

    The dimeric peptide 1 (BVD-74D, as a diastereomeric mixture) is a potent and selective neuropeptide Y Y4 receptor agonist. It represents a valuable candidate in developing traceable ligands for pharmacological studies of Y4 receptors and as a lead compound for antiobesity drugs. Its optically pure stereoisomers along with analogues and fluorescently labeled variants were prepared by exploiting alkene metathesis reactions. The (2R,7R)-diaminosuberoyl containing peptide, (R,R)-1, had markedly higher affinity and agonist efficacy than its (S,S)-counterpart. Furthermore, the sulfo-Cy5 labeled (R,R)-14 retained high agonist potency as a novel fluorescent ligand for imaging Y4 receptors.

  12. Selective increase of dark phase water intake in neuropeptide-Y Y2 and Y4 receptor knockout mice

    OpenAIRE

    Wultsch, Thomas; Painsipp, Evelin; Donner, Sabine; Sperk1, Günther; Herzog, Herbert; Peskar, Bernhard A; Holzer, Peter

    2005-01-01

    Neuropeptide-Y (NPY) is involved in the regulation of ingestive behaviour and energy homeostasis. Since deletion of the NPY Y2 and Y4 receptor gene increases and decreases food intake, respectively, we examined whether water intake during the light and dark phase is altered in Y2 and Y4 receptor knockout mice. The water consumption of mice staying in their home cages was measured by weighing the water bottles at the beginning and end of the light phase during 4 consecutive days. Control, Y2 a...

  13. 抗盐钻井液在舞阳盐矿Y4井的应用%Application of salt resistant slurry on Well Y4 in Wuyang mine

    Institute of Scientific and Technical Information of China (English)

    刘鸿燕; 胡郁乐; 潘峰; 符碧犀

    2011-01-01

    为了解决盐膏层钻井难的问题,针对河南省舞阳地区的含盐地层和舞阳Y4井钻井液应用中暴露的问题,通过对盐水钻井液配方及添加剂作用机理的研究和盐水钻井液优配原则分析,设计出了一种高矿化度和强抑制性的饱和抗盐钻井液体系,添加剂仅为低黏Na-CMC、SMP-Ⅱ、水解聚丙烯腈钾盐和磺化沥青4种,成功完成了Y4井的钻探施工.%In order to overcome the difficulties in salt formation drilling, especially for Wuyang area, and to solve the problem exposed in slurry application of Well Y4 in Wuyang, a type of saturated salt resistant slurry system is developed, through the study of salt water drilling fluid formulation and the working principles of additives, and the analysis on the priority principles of the saline mud. The slurry makes Well Y4 drilling a success. It not only meets the needs of conventional salt formation drilling, but also is adaptive, environmental-friendly and economical.

  14. Impact of Gap Junctional Intercellular Communication on MLO-Y4 Sclerostin and Soluble Factor Expression.

    Science.gov (United States)

    York, S L; Sethu, P; Saunders, M M

    2016-04-01

    Bone remodeling is a continual process in which old bone is resorbed by osteoclasts and new bone is formed by osteoblasts, providing a mechanism for bones' ability to adapt to changes in its mechanical environment. While the role of osteoblasts and osteoclasts in bone remodeling is well understood, the cellular regulation of bone remodeling is unclear. One theory is that osteocytes, found within bone, play an important role in controlling the bone remodeling response. Osteocytes possess gap junctions, narrow channels that extend between nearby cells and allow communication between cells via the transfer of small molecules and ions. This work investigated the potential role of gap junctional intercellular communication in bone remodeling by exposing osteocyte-like MLO-Y4 cells to mechanical strains and quantifying the expression of soluble factors, including sclerostin, a protein closely associated with bone remodeling. The soluble factors and sclerostin expression were further examined after inhibiting gap junctional intercellular communication to study the impact of the communication. At supraphysiologic strains, the inhibition of gap junctional intercellular communication led to increases in sclerostin expression relative to cells in which communication was present, indicating that the communication may play a significant role in regulating bone remodeling. PMID:26154422

  15. Corrosion behavior of as-cast Mg_(68)Zn_(28)Y_4 alloy with I-phase

    Institute of Scientific and Technical Information of China (English)

    SHI Fei; YU Yuan-chun; GUO Xue-feng; ZHANG Zhong-ming; LI Ying-ying

    2009-01-01

    Mg_(68)Zn_(28)Y_4 alloys with stable icosahedral quasicrystals (Zn_(60)Mg_(30)Y_(10)) were prepared by cast method. By simulating the environment of ocean, the alloy was eroded in 3.5% (mass fraction) NaCl for 2, 4 and 30 h. The microstructures of the samples and eroded alloys were analyzed by OM and SEM. The compositions and the quasiperiodic structures were identified respectively by EDS and TEM. And the corrosion potential and corrosion current density before and after immersion were measured by potentiodynamic polarization measurements in 3.5% NaCl. The results show that I-phases grow in the mode of conglomeration, piling and transfixion. The Mg_7Zn_3 matrix and ((Mg) solid solution are eroded badly, while W-phase is eroded partially. At the same time, the I-phases exhibit excellent corrosion resistance property. The resistance to corrosion of Mg_(68)Zn_(28)Y_4 alloy is improved by increasing exposed I-phases. With adding element Y to Mg68Zn32 alloy, the corrosion current is decreased by one order of magnitude. And after the immersion of as-cast Mg_(68)Zn_(28)Y_4 alloy for 30 h, the corrosion current density is reduced by two orders of magnitude compared with that of uneroded Mg_(68)Zn_(32) alloy.

  16. Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva

    Directory of Open Access Journals (Sweden)

    Piras Vincenzo

    2006-06-01

    Full Text Available Abstract Background The aim of our study is to describe a fast molecular method, able to distinguish and quantize the two different genotypes (652 and JP2 of an important periodontal pathogen: Actinobacillus actinomycetemcomitans. The two genotypes show differences in the expression of an important pathogenic factor: the leukotoxin (ltx. In order to evidence this, we performed a real time PCR procedure on the ltx operon, able to recognize Aa clinical isolates with different leukotoxic potentials. Methods The specificity of the method was confirmed in subgingival plaque and saliva specimens collected from eighty-one Italian (Sardinian subjects with a mean age of 43.9, fifty five (68 % of whom had various clinical forms of periodontal disease. Results This procedure showed a good sensitivity and a high linear dynamic range of quantization (107-102 cells/ml for all genotypes and a good correlation factor (R2 = 0.97–0.98. Compared with traditional cultural methods, this real time PCR procedure is more sensitive; in fact in two subgingival plaque and two positive saliva specimens Aa was only detected with the molecular method. Conclusion A low number of Sardinian patients was found positive for Aa infections in the oral cavity, (just 10 positive periodontal cases out of 81 and two of these were also saliva positive. The highly leukotoxic JP2 strain was the most representative (60 % of the positive specimens; the samples from periodontal pockets and from saliva showed some ltx genotype for the same patient. Our experience suggests that this approach is suitable for a rapid and complete laboratory diagnosis for Aa infection.

  17. The absence of protein Y4yS affects negatively the abundance of T3SS Mesorhizobium loti secretin, RhcC2, in bacterial membranes

    Directory of Open Access Journals (Sweden)

    Virginia eMercante

    2015-01-01

    Full Text Available Mesorhizobium loti MAFF303099 has a functional type III secretion system (T3SS that is involved in the determination of nodulation competitiveness on Lotus. The M. loti T3SS cluster contains gene y4yS (mlr8765 that codes for a protein of unknown function (Y4yS. A mutation in the y4yS gene favors the M. loti symbiotic competitive ability on Lotus tenuis cv. Esmeralda and affects negatively the secretion of proteins through T3SS. Here we localize Y4yS in the bacterial membrane using a translational reporter peptide fusion. In silico analysis indicated that this protein presents a tetratricopeptide repeat (TPR domain, a signal peptide and a canonical lipobox LGCC in the N-terminal sequence. These features that are shared with proteins required for the formation of the secretin complex in type IV secretion systems and in the Tad system, together with its localization, suggest that the y4yS-encoded protein is required for the formation of the M. loti T3SS secretin (RhcC2 complex. Remarkably, analysis of RhcC2 in the wild-type and M. loti y4yS mutant strains indicated that the absence of Y4yS affects negatively the accumulation of normal levels of RhcC2 in the membrane.

  18. Inhibition of ABCA1 Protein Expression and Cholesterol Efflux by TNF α in MLO-Y4 Osteocytes.

    Science.gov (United States)

    Wehmeier, Kent R; Kurban, William; Chandrasekharan, Chandrikha; Onstead-Haas, Luisa; Mooradian, Arshag D; Haas, Michael J

    2016-06-01

    Hip fracture and myocardial infarction cause significant morbidity and mortality. In vivo studies raising serum cholesterol levels as well as pro-inflammatory cytokines such as TNF α manifest bone loss and atherosclerotic vascular disease, suggesting that abnormalities of cholesterol transport may contribute to osteoporosis. We used the mouse osteocyte cell line (MLO-Y4) to investigate the effects of TNF α on the expression of cholesterol acceptor proteins such as apolipoprotein A-I (apo A-I) and apolipoprotein E (apo E), as well as on the cholesterol transporters ATP-binding cassette-1 (ABCA1), scavenger receptor class B type 1 (SRB1), and cluster of differentiation 36 (CD36). MLO-Y4 cells do not express apo A-I or apo E; however, they do express all three cholesterol transporters (ABCA1, SRB1, and CD36). Treatment of MLO-Y4 cells with TNF α had no effect on SRB1, CD36, and osteocalcin levels; however, TNF α reduced ABCA1 protein levels in a dose-dependent manner and cholesterol efflux to apo A-I. Interestingly, TNF α treatment increased ABCA1 promoter activity and ABCA1 mRNA levels, and increased liver X receptor α protein expression, but had no effect on retinoid X receptor α and retinoic acid receptor α levels. Pharmacological inhibition of p38 mitogen-activated protein (MAP) kinase, but not c-jun-N-terminal kinase 1 or mitogen-activated protein kinase (MEK), restored ABCA1 protein levels in TNF α-treated cells. These results suggest that pro-inflammatory cytokines regulate cholesterol metabolism in osteocytes in part by suppressing ABCA1 levels post-translationally in a p38 MAP kinase-dependent manner. PMID:26759003

  19. Sol-gel synthesis and luminescence of Y4Al2O9:RE3+(RE=Eu,Tb)

    International Nuclear Information System (INIS)

    Y4Al2O9 (YAM) was prepared by a sol-gel process, using yttrium and aluminum citrate complexes as precursors. The sol-gel process produced single-phase YAM at 900 C, as opposed to the conventional solid-slate reaction, which led to the formation of other phases, even if at 1600 C. The emission and excitation spectra of Eu3+ and Tb3+ in YAM showed the existence of two luminescence centers, agreeing with the crystal structure of YAM. The spectral properties of the samples are discussed. (orig.)

  20. Preparation and Electrorheological Property of Y4O(OH)9(NO3)-NH4NO3 Materials

    Institute of Scientific and Technical Information of China (English)

    Ma Shuzhen; Huo Li; Jia Yunling; Shang Yanli; Li Shuxin; Xu Mingyuan; Li Junran; Zhang Shaohua

    2006-01-01

    The new electrorheological (ER) material, a particle material composed of Y4O(OH)9(NO3) and NH4NO3, was obtained.They display better ER performance.The shear stress of the suspension of Y4O(OH)9(NO3)(NH4NO3)2.8 material in dimethyl silicone oil reaches 1469 Pa at an electric field strength (E) of 4.2 kV·mm-1 and the shear rate (γ) of 150 s-1.The relative shear stress, τE/τ0 (τE and τ0 are the shear stresses at E=4.2 and 0 kV·mm-1, respectively), is up to 29, which is 19 times that of pure Y2O3 material.The dielectric and conductive property of the materials play important roles in the modification of the ER effect of the particle materials.The researches on these new ER materials are very useful for obtaining a better understanding on the mechanism of the ER effect and finding an ideal ER material.

  1. Emission analysis of RE3+ (RE=Eu, Sm, Dy):MgY4Si3O13 phosphors

    International Nuclear Information System (INIS)

    A series of Eu3+, Sm3+, and Dy3+ ion doped magnesium yttrium silicate [MgY4Si3O13] phosphors was synthesized using the solid-state reaction method with a grain size of approximately 500 nm in hexagonal symmetry. The photoluminescence (PL) spectra of the Eu3+, Sm3+, and Dy3+:MgY4Si3O13 phosphors exhibit bright red, orange-red and yellow emissions at 615 nm (5D0→7F2), 603 nm (4G52→6H7/2), and 574 nm (4F9/2→6H13/2), respectively. The thermoluminescence (TL) of the phosphors displays the maximum intensity consistently at 10 mol% of the ion concentrations, with a single glow peak around 165 °C (Eu3+-activated phosphor), 230 °C (Sm3+-activated phosphor), and 230 °C (Dy3+-activated phosphor), respectively. The trap parameters such as the order of kinetics (b), activation energy (E), frequency factor (S), and Balarin parameter (γ) associated with the most intensive glow peak of these phosphors were finally determined by analyzing their line-shapes on the basis of Chen's method

  2. Biosynthesis and NMR-studies of a double transmembrane domain from the Y4 receptor, a human GPCR

    Energy Technology Data Exchange (ETDEWEB)

    Zou Chao [University of Zurich, Institute of Organic Chemistry (Switzerland); Naider, Fred [College of Staten Island, CUNY, Department of Chemistry (United States); Zerbe, Oliver [University of Zurich, Institute of Organic Chemistry (Switzerland)], E-mail: oliver.zerbe@oci.uzh.ch

    2008-12-15

    The human Y4 receptor, a class A G-protein coupled receptor (GPCR) primarily targeted by the pancreatic polypeptide (PP), is involved in a large number of physiologically important functions. This paper investigates a Y4 receptor fragment (N-TM1-TM2) comprising the N-terminal domain, the first two transmembrane (TM) helices and the first extracellular loop followed by a (His){sub 6} tag, and addresses synthetic problems encountered when recombinantly producing such fragments from GPCRs in Escherichia coli. Rigorous purification and usage of the optimized detergent mixture 28 mM dodecylphosphocholine (DPC)/118 mM% 1-palmitoyl-2-hydroxy-sn-glycero-3-[phospho-rac-(1-glycerol)] (LPPG) resulted in high quality TROSY spectra indicating protein conformational homogeneity. Almost complete assignment of the backbone, including all TM residue resonances was obtained. Data on internal backbone dynamics revealed a high secondary structure content for N-TM1-TM2. Secondary chemical shifts and sequential amide proton nuclear Overhauser effects defined the TM helices. Interestingly, the properties of the N-terminal domain of this large fragment are highly similar to those determined on the isolated N-terminal domain in the presence of DPC micelles.

  3. Scintillation properties of μPD-grown Y4Al2O9:Pr (YAM:Pr) crystals

    International Nuclear Information System (INIS)

    Highlights: • YAM:Pr crystals do scintillate and as such deserve further interest. • Fast d–f luminescence of Pr3+ ions appears in X-ray excited spectra. • Two components (24 and 790 ns) constitute scintillation time profiles. - Abstract: Y4Al2O9:Pr (YAM:Pr) crystals have been grown by the micro-pulling-down method and their scintillation properties have been investigated. YAM:0.1%Pr displays a light yield of about 2000 ph/MeV and its scintillation time profile contains a prompt component with a decay time of 23.5 ns and a contribution of 20%. Radioluminescence spectra show both fast d–f and slow f–f praseodymium emissions. Low temperature glow curves are complex, consisting of discrete peaks and broad bands related to quasi-continuous trap distributions. Overall scintillation performance of YAM:Pr deteriorates with increasing praseodymium concentration

  4. Influence of complement on neutrophil extracellular trap release induced by bacteria

    DEFF Research Database (Denmark)

    Palmer, Lisa Joanne; Damgaard, Christian; Holmstrup, Palle;

    2016-01-01

    by Staphylococcus aureus and three oral bacteria: Actinomyces viscosus, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum subsp. vincettii. MATERIAL AND METHODS: Bacteria-stimulated NET release from the neutrophils of healthy donors was measured fluorometrically. Various complement containing....... Within biofilms, the complement-inactivating abilities of some bacteria may protect other species against NETosis, while these are more vulnerable when adopting a planktonic lifestyle....

  5. Changes in oral microflora after full-mouth tooth extraction : A prospective cohort study

    NARCIS (Netherlands)

    de Waal, Yvonne C M; Winkel, Edwin G; Raangs, Gerwin C; van der Vusse, Marleen L; Rossen, John W A; van Winkelhoff, Arie Jan

    2014-01-01

    AIM: The aim of the study was to evaluate the effect of full-mouth tooth extraction on the oral microflora, with emphasis on the presence and load of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. MATERIAL AND METHODS: Adult patients (n = 30), with moderate to advanced periodont

  6. Antibacterial activity of the lactoperoxidase system combined with edible Laminaria hot-water extract as a source of halide ions.

    Science.gov (United States)

    Shin, Kouichirou; Nakano, Manabu; Yamauchi, Koji; Toida, Tomohiro; Iwatsuki, Keiji

    2012-01-01

    Hot-water extracts prepared from nine out of 12 samples of dried edible Laminaria reduced the viable numbers of Aggregatibacter actinomycetemcomitans, Staphylococcus aureus, and Esherichia coli below the detection limit after incubation for 5 min when combined with lactoperoxidase, glucose oxidase, and glucose. Some extracts showed higher bactericidal activity and a higher OI(-) concentration in the assay mixture after ultrafiltration.

  7. Microstructure, corrosion resistance and cytocompatibility of Mg-5Y-4Rare Earth-0.5Zr (WE54) alloy

    Energy Technology Data Exchange (ETDEWEB)

    Smola, Bohumil, E-mail: bohumil.smola@mff.cuni.cz [Charles University Prague, Faculty of Mathematics and Physics, Ke Karlovu 5, 121 16 Praha 2 (Czech Republic); Joska, Ludek [Institute of Chemical Technology Prague, Faculty of Chemical Technology, Technicka 5, 166 28 Praha 6 (Czech Republic); Brezina, Vitezslav [University of South Bohemia, Institute of Physical Biology, Zamek 136, 373 33 Nove Hrady (Czech Republic); Stulikova, Ivana [Charles University Prague, Faculty of Mathematics and Physics, Ke Karlovu 5, 121 16 Praha 2 (Czech Republic); Hnilica, Frantisek [Czech Technical University in Prague, Faculty of Mechanical Engineering, Karlovo namesti 13, 121 35 Praha 2 (Czech Republic)

    2012-05-01

    Conventionally cast Mg-5Y-4Rare Earth-0.5Zr alloy (WE54) was solution treated (525 Degree-Sign C/8 h - T4) and one part subsequently aged (200 Degree-Sign C/16 h - T6). Powder from the cast WE54 alloy prepared by gas atomizing was consolidated by extrusion at 250 Degree-Sign C or 400 Degree-Sign C. Dense triangular arrangement of prismatic plates of transient D0{sub 19} and C-base centered orthorhombic phases precipitated in the {alpha}-Mg matrix during the T6 treatment. Both alloys prepared by powder metallurgy exhibit similar microstructure consisting of {approx} 4-6 {mu}m {alpha}-Mg matrix fibers surrounded by particles of the equilibrium Mg{sub 5}(Y, Nd) phase and of oxides. Open circuit potential and polarization resistance in the isotonic saline (9 g/l NaCl/H{sub 2}O) were monitored for 24 h. The corrosion rate of the T4 and T6 treated alloys was about 80 times lower than that of commercial Mg. Both alloys prepared by powder metallurgy exhibited approximately 8 times higher corrosion resistance than commercial Mg. The human MG-63 osteoblast-like cells spreading and division in the extracts (0.28 g in 28 ml of EMEM) of all 4 alloys were monitored by cinemicrography for 24 h. The MG-63 cells proliferate without cytotoxicity in all extracts. - Highlights: Black-Right-Pointing-Pointer T6 treated WE54 alloy exhibit dense triangular arrangement of {beta} Double-Prime and {beta} Prime phase prismatic plates. Black-Right-Pointing-Pointer Microstructure of PM prepared WE54 alloy consists of {alpha}-Mg phase cells surrounded by {beta} phase particles. Black-Right-Pointing-Pointer PM produced WE54 corroded 10 times faster in physiological solution thanT4 and T6 treated WE54. Black-Right-Pointing-Pointer MG63 cell spreading in EMEM extracts of PM prepared WE54 is comparable to that in control EMEM. Black-Right-Pointing-Pointer Cell mitosis is enhanced in PM WE54 extracts compared to the control and extracts of T4 and T6 WE54.

  8. The Comparison of Biological Characteristics between Osteocyte-like Cell MLO-Y4 and Osteoblast-like Cell MC3T3-E1%骨样细胞MLO-Y4与成骨样细胞MC3T3-E1生物学特性的比较

    Institute of Scientific and Technical Information of China (English)

    瓮媛媛; 续惠云; 安龙; 商澎

    2010-01-01

    分别采用倒置显微镜观察法、细胞计数法、RT-PCR法、磷酸对硝基苯酚法(PNPP法)和ELISA法来比较小鼠骨样细胞MLO-Y4与小鼠成骨样细胞MC3T3-E1的细胞形态、增殖、相关基因的表达和分泌功能的差异.结果显示MC3T3-E1细胞呈长梭形,具有少量短的突触;而MLO-Y4细胞呈星状或树枝状且具有很多长的突触.MC3T3-E1细胞的增殖能力强于MLO-Y4细胞,两者的倍增时间分别是18 h和20 h.MC3T3-E1细胞中原癌基因c-fos和骨桥蛋白基因OPN mRNA的表达明显高于MLO-Y4细胞,而骨钙素基因OC mRNA的表达则是MC3T3-E1细胞远低于MLO-Y4细胞,白细胞分化抗原44基因CD44 mRNA在两种细胞中的表达差异不明显.ALP的分泌在MC3T3-E1细胞中高于MLO-Y4细胞,NO的分泌在两种细胞中没有显著性差异,M-CSF在MLO-Y4细胞中的分泌较高.由此可见骨样细胞MLO-Y4与成骨样细胞MC3T3-E1在形态、ALP和M-CSF分泌及c-fos、OPN和OC mRNA表达方面差异明显.

  9. Regulation of gene expression and subcellular protein distribution in MLO-Y4 osteocytic cells by lysophosphatidic acid: Relevance to dendrite outgrowth

    OpenAIRE

    Waters, Katrina M.; Jon M Jacobs; Gritsenko, Marina A.; Karin, Norman J.

    2011-01-01

    Osteoblastic and osteocytic cells are highly responsive to the lipid growth factor lysophosphatidic acid (LPA) but the mechanisms by which LPA alters bone cell functions are largely unknown. A major effect of LPA on osteocytic cells is the stimulation of dendrite membrane outgrowth, a process that we predicted to require changes in gene expression and protein distribution. We employed DNA microarrays for global transcriptional profiling of MLO-Y4 osteocytic cells grown for 6 and 24 hours in t...

  10. 骨碎补总黄酮对 MLO-Y4细胞增殖、分化、矿化和凋亡影响的探究%Effect of drynaria total flavonoids on the proliferation, differentiation, mineralization, and apoptosis of MLO-Y4 cells

    Institute of Scientific and Technical Information of China (English)

    李洋; 康倩; 荣婵; 舒晓春

    2015-01-01

    Objective To investigate the effect of drynaria total flavonoids on the proliferation, differentiation, mineralization, and apoptosis of MLO-Y4 cells.Methods MLO-Y4 cells were cultured with different concentrations (1, 10, 100 mg/l) of drynaria total flavonoids in vitro, and MC3T3-E1 cells were cultured as a control.The proliferation and differentiation of MLO-Y4 cells were examined using CCK-8 method and the alkaline phosphatase ( ALP) kit, respectively.The mineralization was detected using Alizarin red staining.DAPI staining and flow cytometry were used to reflect the cell apoptosis induced by etoposide.Results The most effective concentration of the drynaria total flavonoids on the proliferation and differentiation of MLO-Y4 cells were 1 mg/l and 10 mg/l, respectively.The concentration of 100mg/l did not stimulate cell proliferation and ALP activity.There was no effect on the formation of calcium nodules with all concentrations.Concentrations of 1 and 10 mg/l inhibited the apoptosis to a certain extent.In contrary, concentration of 100 mg/l played a role in invoking cell apoptosis.Conclusion Certain concentrations of drynaria total flavonoids can promote the proliferation and differentiation of MLO-Y4 cells, and can inhibit the cell apoptosis.%目的:研究骨碎补总黄酮对MLO-Y4类骨细胞系增殖、分化、矿化以及凋亡的影响。方法体外培养MLO-Y4细胞,并以MC3T3-E1细胞作对照细胞,分别用不同质量浓度(1,10,100 mg/l)的骨碎补总黄酮干预,采用CCK-8法以及ALP试剂盒检测MLO-Y4细胞的增殖和分化情况;用茜素红染色法观察矿化结节的形成;DAPI染色和流式细胞术定性和定量反映依托泊苷诱导的细胞凋亡情况。结果1,10 mg/l浓度组的骨碎补总黄酮能促进MLO-Y4细胞的增殖,并且能够一定程度上促进细胞ALP的合成分泌,100 mg/l组不具有促进细胞增殖,ALP合成分泌的作用。三个浓度组均无影响MLO-Y4

  11. 流体剪切力对MLO-Y4骨细胞骨性标志物表达的影响%Effect of Fluid Shear Stress on the expression of bone markers in MLO-Y4 Osteocyte-Like Cells

    Institute of Scientific and Technical Information of China (English)

    崔亮; 杨雁琪; 李小彤; 张丁; 傅民魁

    2007-01-01

    目的 通过研究MLO-Y4骨细胞受流体剪切力作用前后骨性标志物在mRNA水平的表达变化,探讨机械力影响骨组织代谢中骨细胞的作用.方法 以小鼠成骨细胞(MOB)为参照,逆转录聚合酶链式反应(RT-PCR)检测MLO-Y4细胞在体外培养条件下,以及受流体剪切力作用0.5 h,1 h,2 h,4 h,6 h,12 h,24 h后骨性标志物在mRNA水平的表达特征和变化.结果 以MOB为参照,在mRNA水平,体外培养条件下MLO-Y4细胞骨钙素(OCN)、碱性磷酸酶(ALP)和骨桥蛋白(OPN)高表达;破骨细胞分化因子(RANKL)、骨保护因子(OPG)低表达,但RANKL/OPG比值明显高于MOB.受流体剪切力作用后,MLO-Y4细胞ALP、OCN表达降低;RANKL和OPN表达增加;OPG随加力时间也有一定变化.结论 MLO-Y4细胞具有分化终末骨细胞的特点;流体剪切力作用后,RANKL/OPG比值以及ALP、OCN和OPN等骨性标志物的表达发生变化,为其参与机械力信号到生物信号的传导过程提供了间接证据.

  12. Effects of load ratio, R, and test temperature on high cycle fatigue behavior of nano-structured Al-4Y-4Ni-X alloy composites

    Energy Technology Data Exchange (ETDEWEB)

    El-Shabasy, Adel B., E-mail: ashabasy@hotmail.com [Department of Design and Production Engineering, Faculty of Engineering, Ain Shams University, Cairo 11517 (Egypt); Hassan, Hala A. [Department of Design and Production Engineering, Faculty of Engineering, Ain Shams University, Cairo 11517 (Egypt); Lewandowski, John J. [Department of Material' s Science and Engineering, Case Western Reserve University, Cleveland, OH 44106 (United States)

    2012-12-15

    Nanostructured Al-4Y-4Ni-X composites created by extruding atomized amorphous powders at different extrusion ratios were tested under high cycle bending fatigue at load ratios, R=0.1, 0.33 and -1 at room temperature, 149 Degree-Sign C and 260 Degree-Sign C. Increasing the extrusion ratio generally improved the fatigue life and the fatigue limits were well in excess of that obtained on conventional aluminum alloys at all temperatures tested. The fatigue limits obtained in this work were also compared to previously reported values for a nanostructured composite Al-Gd-Ni-Fe alloy produced via similar means.

  13. Regulation of gene expression and subcellular protein distribution in MLO-Y4 osteocytic cells by lysophosphatidic acid: Relevance to dendrite outgrowth.

    Energy Technology Data Exchange (ETDEWEB)

    Waters, Katrina M.; Jacobs, Jon M.; Gritsenko, Marina A.; Karin, Norman J.

    2011-02-26

    Osteoblastic and osteocytic cells are highly responsive to the lipid growth factor lysophosphatidic acid (LPA) but the mechanisms by which LPA alters bone cell functions are largely unknown. A major effect of LPA on osteocytic cells is the stimulation of dendrite membrane outgrowth, a process that we predicted to require changes in gene expression and protein distribution. We employed DNA microarrays for global transcriptional profiling of MLO-Y4 osteocytic cells grown for 6 and 24h in the presence or absence of LPA. We identified 932 transcripts that displayed statistically significant changes in abundance of at least 1.25-fold in response to LPA treatment. Gene ontology (GO) analysis revealed that the regulated gene products were linked to diverse cellular processes, including DNA repair, response to unfolded protein, ossification, protein-RNA complex assembly, and amine biosynthesis. Gene products associated with the regulation of actin microfilament dynamics displayed the most robust expression changes, and LPA-induced dendritogenesis in vitro was blocked by the stress fiber inhibitor cytochalasin D. Mass spectrometry-based proteomic analysis of MLO-Y4 cells revealed significant LPA-induced changes in the abundance of 284 proteins at 6h and 844 proteins at 24h. GO analysis of the proteomic data linked the effects of LPA to cell processes that control of protein distribution and membrane outgrowth, including protein localization, protein complex assembly, Golgi vesicle transport, cytoskeleton-dependent transport, and membrane invagination/endocytosis. Dendrites were isolated from LPA-treated MLO-Y4 cells and subjected to proteomic analysis to quantitatively assess the subcellular distribution of proteins. Sets of 129 and 36 proteins were enriched in the dendrite fraction as compared to whole cells after 6h and 24h of LPA exposure, respectively. Protein markers indicated that membranous organelles were largely excluded from the dendrites. Highly represented among

  14. INFLUENCIA DE LA QUÍMICA SUPERFICIAL EN LA ENTALPÍA DE INMERSIÓN DE CARBONES ACTIVADOS EN SOLUCIONES ACUOSAS DE FENOL Y 4-NITRO FENOL

    OpenAIRE

    Luisa Fernanda Navarrete; Liliana Giraldo; Juan Carlos Moreno

    2008-01-01

    Se estudian las interacciones de cinco muestras de carbón activado obtenidas a partir de diferentes materiales lignocelulósicos, con diferente grado de activación alrededor de 20%, con soluciones acuosas de fenol y 4-nitro fenol mediante la determinación de las entalpías de inmersión. Se establece que los carbones activados obtenidos son de carácter básico y presentan valores para el punto de carga cero (PZC), que varían entre 7,4...

  15. Luminescence from the 5D1,2,3 excited states of Eu3+ in Y4Al2O9 crystal

    Science.gov (United States)

    Kaczkan, Marcin

    2016-09-01

    The site-selective emission originating from 5D1,2,3 energy levels of Eu3+ in Y4Al2O9 (YAM) monoclinic bulk crystal is investigated. Energy and Stark splitting of excited states of europium in YAM are determined based on the low temperature absorption and emission spectra. Luminescence decays of three different sites of Eu3+ ions are measured as a function of temperature and europium concentration. The cross-relaxation among the Eu3+ ions are observed and discussed. Non-resonant mechanisms responsible for the temperature quenching of 5D1 emission are proposed.

  16. Quercetin inhibits inflammatory bone resorption in a mouse periodontitis model.

    Science.gov (United States)

    Napimoga, Marcelo H; Clemente-Napimoga, Juliana T; Macedo, Cristina G; Freitas, Fabiana F; Stipp, Rafael N; Pinho-Ribeiro, Felipe A; Casagrande, Rubia; Verri, Waldiceu A

    2013-12-27

    Periodontitis is a disease that leads to bone destruction and represents the main cause of tooth loss in adults. The development of aggressive periodontitis has been associated with increased inflammatory response that is induced by the presence of a subgingival biofilm containing Aggregatibacter actinomycetemcomitans. The flavonoid quercetin (1) is widespread in vegetables and fruits and exhibits many biological properties for possible medical and clinical applications such as its anti-inflamatory and antioxidant effects. Thus, in the present study, the properties of 1 have been evaluated in bone loss and inflammation using a mouse periodontitis model induced by A. actinomycetemcomitans infection. Subcutaneous treatment with 1 reduced A. actinomycetemcomitans-induced bone loss and IL-1β, TNF-α, IL-17, RANKL, and ICAM-1 production in the gingival tissue without affecting bacterial counts. These results demonstrated that quercetin exhibits protective effects in A. actinomycetemcomitans-induced periodontitis in mice by modulating cytokine and ICAM-1 production.

  17. SNOWMASS WHITE PAPER - SLHC Endcap 1.4<y<4 Hadron Optical Calorimetry Upgrades in CMS with Applications to NLC/T-LEP, Intensity Frontier, and Beyond

    CERN Document Server

    Bilki, Burak; Winn, David R; Yetkin, Taylan

    2013-01-01

    Radiation damage in the plastic scintillator and/or readout WLS fibers in the HE endcap calorimeter 1.4<y<4 in the CMS experiment at LHC and SLHC will require remediation after 2018. We describe one alternative using the existing brass absorber in the Endcap calorimeter, to replace the plastic scintillator tiles with BaF2 tiles, or quartz tiles coated with thin(1-5 micron) films of radiation-hard pTerphenyl(pTP) or the fast phosphor ZnO:Ga. These tiles would be read-out by easily replaceable arrays of straight, parallel WLS fibers coupled to clear plastic-cladded quartz fibers of proven radiation resistance. We describe a second alternative with a new absorber matrix extending to 1.4<y<4 in a novel Analog Particle Flow Cerenkov Compensated Calorimeter, using a dual readout of quartz tiles and scintillating (plastic, BaF2, or pTP/ ZnO:Ga thin film coated quartz, or liquid scintillator) tiles, also using easily replaceable arrays of parallel WLS fibers coupled to clear quartz transmitting fibers for...

  18. 周期性压应力作用下MLO-Y4细胞基因差异表达的初步研究

    Institute of Scientific and Technical Information of China (English)

    何陨; 朱智敏; 陈文川; 王航; 方园; 李磊

    2012-01-01

    目的:本研究对骨细胞实施应力刺激,进行基因表达谱检测,从基因转录组水平阐明MLO-Y4细胞的力学敏感基因,考察加载应力的力值、频率、作用时间对力学敏感基因表达的影响以及三者之间的相互作用。方法:本实验运用小鼠全基因组寡核苷酸芯片、qRT-PCR等方法,筛选出CCS(周期性压应力)作用下MLO-Y4细胞的差异表达基因,并进行验证,考察IL-6的mRNA表达变化与应力力值、频率、作用时间的关系。结果:1.芯片和qRT-PCR检测出的基因表达率具有良好的相关性,其皮尔森线性相关值R=0.903。2.最具统计学意义的GO Terms是“趋化因子活性”;出现频率最高及最具统计学意义的KEGG通路是“MAPK”和“细胞因子.细胞因子受体相互作用”。3.在CCS作用下MLO—Y4细胞IL-6mRNA的表达受压应力力值、频率和作用时间的影响:当三个变量中的两个一定时,IL-6mRNA的表达随着第三个变量的增加而增加。结论:1.CCS作用下MLO-Y4细胞差异表达基因(以IL-6为代表)以表达上调为主,且与破骨细胞和骨吸收密切相关。2.在CCS作用下.MLO-Y4细胞IL-6mRNA表达受压应力力值、频率和作用时间的共同影响。

  19. Nd(III) and Yb(III) ions incorporated in Y4Al2O9 obtained by sol-gel method: synthesis, structure, crystals and luminescence

    International Nuclear Information System (INIS)

    The nanocrystalline powders of Y4Al2O9 (YAM) pure and doped by Nd, Yb and codoped by Nd and Yb were obtained via modified sol-gel method. These powders were characterized by X-ray diffraction method, scanning electron microscopy and high resolution scanning electron microscopy, luminescence spectroscopy and differential thermal analysis (DTA). We obtained single phase powders of crystalline structure with average size 70 nm exhibiting interesting luminescent properties. Efficient non-radiative energy transfer between Nd and Yb was found. DTA confirmed the phase transition at about 1400 C. From these nanocrystalline powders, the crystals YAM:Yb, YAM:Yb,Nd were grown by micro-pulling down technique. They were cracking during cooling owing to the phase transition. Luminescent properties of YAM:Nd,Yb crystals were identical with properties of corresponding nanopowders within experimental incertitude. (copyright 2009 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim) (orig.)

  20. Activation of a P2Y4-like purinoceptor triggers an increase in cytosolic [Ca2+] in the red blood cells of the lizard Ameiva ameiva (Squamata, Teiidae

    Directory of Open Access Journals (Sweden)

    Sartorello R.

    2005-01-01

    Full Text Available An increasing number of pathophysiological roles for purinoceptors are emerging, some of which have therapeutic potential. Erythrocytes are an important source of purines, which can be released under physiological and physiopathological conditions, acting on purinergic receptors associated with the same cell or with neighboring cells. Few studies have been conducted on lizards, and have been limited to ATP agonist itself. We have previously shown that the red blood cells (RBCs of the lizard Ameiva ameiva store Ca2+ in the endoplasmic reticulum (ER and that the purinergic agonist ATP triggers a rapid and transient increase of [Ca2+]c by mobilization of the cation from internal stores. We also reported the ability of the second messenger IP3 to discharge the ER calcium pool of the ER. Here we characterize the purinoceptor present in the cytoplasmic membrane of the RBCs of the lizard Ameiva ameiva by the selective use of ATP analogues and pyrimidine nucleotides. The nucleotides UTP, UDP, GTP, and ATPgammaS triggered a dose-dependent response, while interestingly 2MeSATP, 2ClATP, alpha, ß-ATP, and ADP failed to do so in a 1- to 200-µm con- centration. The EC50 obtained for the compounds tested was 41.77 µM for UTP, 48.11 µM for GTP, 53.11 µM for UDP, and 30.78 µM for ATPgammaS. The present data indicate that the receptor within the RBCs of Ameiva ameiva is a P2Y4-like receptor due to its pharmacological similarity to the mammalian P2Y4 receptor.

  1. Activation of a P2Y4-like purinoceptor triggers an increase in cytosolic [Ca2+] in the red blood cells of the lizard Ameiva ameiva (Squamata, Teiidae).

    Science.gov (United States)

    Sartorello, R; Garcia, C R S

    2005-01-01

    An increasing number of pathophysiological roles for purinoceptors are emerging, some of which have therapeutic potential. Erythrocytes are an important source of purines, which can be released under physiological and physiopathological conditions, acting on purinergic receptors associated with the same cell or with neighboring cells. Few studies have been conducted on lizards, and have been limited to ATP agonist itself. We have previously shown that the red blood cells (RBCs) of the lizard Ameiva ameiva store Ca2+ in the endoplasmic reticulum (ER) and that the purinergic agonist ATP triggers a rapid and transient increase of [Ca2+]c by mobilization of the cation from internal stores. We also reported the ability of the second messenger IP3 to discharge the ER calcium pool of the ER. Here we characterize the purinoceptor present in the cytoplasmic membrane of the RBCs of the lizard Ameiva ameiva by the selective use of ATP analogues and pyrimidine nucleotides. The nucleotides UTP, UDP, GTP, and ATPgammaS triggered a dose-dependent response, while interestingly 2MeSATP, 2ClATP, alpha, ss-ATP, and ADP failed to do so in a 1- to 200-microm con- centration. The EC50 obtained for the compounds tested was 41.77 microM for UTP, 48.11 microM for GTP, 53.11 microM for UDP, and 30.78 microM for ATPgammaS. The present data indicate that the receptor within the RBCs of Ameiva ameiva is a P2Y4-like receptor due to its pharmacological similarity to the mammalian P2Y4 receptor. PMID:15665982

  2. 17β-estradiol rapidly activates calcium release from intracellular stores via the GPR30 pathway and MAPK phosphorylation in osteocyte-like MLO-Y4 cells

    KAUST Repository

    Ren, Jian

    2012-03-06

    Estrogen regulates critical cellular functions, and its deficiency initiates bone turnover and the development of bone mass loss in menopausal females. Recent studies have demonstrated that 17β-estradiol (E 2) induces rapid non-genomic responses that activate downstream signaling molecules, thus providing a new perspective to understand the relationship between estrogen and bone metabolism. In this study, we investigated rapid estrogen responses, including calcium release and MAPK phosphorylation, in osteocyte-like MLO-Y4 cells. E 2 elevated [Ca 2+] i and increased Ca 2+ oscillation frequency in a dose-dependent manner. Immunolabeling confirmed the expression of three estrogen receptors (ERα, ERβ, and G protein-coupled receptor 30 [GPR30]) in MLO-Y4 cells and localized GPR30 predominantly to the plasma membrane. E 2 mobilized calcium from intracellular stores, and the use of selective agonist(s) for each ER showed that this was mediated mainly through the GPR30 pathway. MAPK phosphorylation increased in a biphasic manner, with peaks occurring after 7 and 60 min. GPR30 and classical ERs showed different temporal effects on MAPK phosphorylation and contributed to MAPK phosphorylation sequentially. ICI182,780 inhibited E 2 activation of MAPK at 7 min, while the GPR30 agonist G-1 and antagonist G-15 failed to affect MAPK phosphorylation levels. G-1-mediated MAPK phosphorylation at 60 min was prevented by prior depletion of calcium stores. Our data suggest that E 2 induces the non-genomic responses Ca 2+ release and MAPK phosphorylation to regulate osteocyte function and indicate that multiple receptors mediate rapid E 2 responses. © 2012 Springer Science+Business Media, LLC.

  3. Interactions of zebrafish peptide YYb with the neuropeptide Y-family receptors Y4, Y7, Y8a and Y8b

    Directory of Open Access Journals (Sweden)

    Görel eSundström

    2013-03-01

    Full Text Available The neuropeptide Y (NPY system influences numerous physiological functions including feeding behavior, endocrine regulation, and cardiovascular regulation. In jawed vertebrates it consists of 3-4 peptides and 4-7 receptors. Teleost fishes have unique duplicates of NPY and PYY as well as the Y8 receptor. In the zebrafish, the NPY system consists of the peptides NPYa, PYYa, and PYYb (NPYb appears to have been lost and at least seven NPY receptors: Y1, Y2, Y2-2, Y4, Y7, Y8a and Y8b. Previously PYYb binding has been reported for Y2 and Y2-2. To search for peptide-receptor preferences, we have investigated PYYb binding to four of the remaining receptors and compared with NPYa and PYYa. Taken together, the most striking observations are that PYYa displays reduced affinity for Y2 (3 nM compared to the other peptides and receptors and that all three peptides have higher affinity for Y4 (0.028-0.034 nM than for the other five receptors. The strongest peptide preference by any receptor selectivity is the one previously reported for PYYb by the Y2 receptor, as compared to NPY and PYYa. These affinity differences may be helpful to elucidate specific details of peptide-receptor interactions. Also, we have investigated the level of mRNA expression in different organs using qPCR. All peptides and receptors have higher expression in heart, kidney and brain. These quantitative aspects on receptor affinities and mRNA distribution help provide a more complete picture of the NPY system.

  4. Simvastatin rescues homocysteine-induced apoptosis of osteocytic MLO-Y4 cells by decreasing the expressions of NADPH oxidase 1 and 2.

    Science.gov (United States)

    Takeno, Ayumu; Kanazawa, Ippei; Tanaka, Ken-Ichiro; Notsu, Masakazu; Yokomoto-Umakoshi, Maki; Sugimoto, Toshitsugu

    2016-04-25

    Clinical studies have shown that hyperhomocysteinemia is associated with bone fragility. Homocysteine (Hcy) induces apoptosis of osteoblastic cell lineage by increasing oxidative stress, which may contribute to Hcy-induced bone fragility. Statins, 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors, ameliorate oxidative stress by regulating oxidant and anti-oxidant enzymes. However, the effects of statins on Hcy-induced apoptosis of osteocytes are unknown. This study was thus aimed to investigate whether or not statins prevent Hcy-induced apoptosis of osteocytic MLO-Y4 cells and regulate NADPH oxidase (Nox) expression. TUNEL staining showed that 5 mM Hcy induced apoptosis of MLO-Y4 cells, and that co-incubation of 10(-9) or 10(-8) M simvastatin significantly suppressed the apoptotic effect. Moreover, we confirmed the beneficial effect of simvastatin against Hcy's apoptotic effect by using a DNA fragment ELISA assay. However, TUNEL staining showed no significant effects of pravastatin, a hydrophilic statin, on the Hcy-induced apoptosis. Real-time PCR showed that Hcy increased the mRNA expressions of Nox1 and Nox2, whereas simvastatin inhibited the stimulation of Nox1 and Nox2 expressions by Hcy. In contrast, neither Hcy nor simvastatin had any effect on Nox4 expression. These findings indicate that simvastatin prevents the detrimental effects of Hcy on the apoptosis of osteocytes by regulating the expressions of Nox1 and Nox2, suggesting that statins may be beneficial for preventing Hcy-induced osteocyte apoptosis and the resulting bone fragility. PMID:26842590

  5. 17β-estradiol rapidly activates calcium release from intracellular stores via the GPR30 pathway and MAPK phosphorylation in osteocyte-like MLO-Y4 cells.

    Science.gov (United States)

    Ren, Jian; Wu, Jun Hua

    2012-05-01

    Estrogen regulates critical cellular functions, and its deficiency initiates bone turnover and the development of bone mass loss in menopausal females. Recent studies have demonstrated that 17β-estradiol (E(2)) induces rapid non-genomic responses that activate downstream signaling molecules, thus providing a new perspective to understand the relationship between estrogen and bone metabolism. In this study, we investigated rapid estrogen responses, including calcium release and MAPK phosphorylation, in osteocyte-like MLO-Y4 cells. E(2) elevated [Ca(2+)]( i ) and increased Ca(2+) oscillation frequency in a dose-dependent manner. Immunolabeling confirmed the expression of three estrogen receptors (ERα, ERβ, and G protein-coupled receptor 30 [GPR30]) in MLO-Y4 cells and localized GPR30 predominantly to the plasma membrane. E(2) mobilized calcium from intracellular stores, and the use of selective agonist(s) for each ER showed that this was mediated mainly through the GPR30 pathway. MAPK phosphorylation increased in a biphasic manner, with peaks occurring after 7 and 60 min. GPR30 and classical ERs showed different temporal effects on MAPK phosphorylation and contributed to MAPK phosphorylation sequentially. ICI182,780 inhibited E(2) activation of MAPK at 7 min, while the GPR30 agonist G-1 and antagonist G-15 failed to affect MAPK phosphorylation levels. G-1-mediated MAPK phosphorylation at 60 min was prevented by prior depletion of calcium stores. Our data suggest that E(2) induces the non-genomic responses Ca(2+) release and MAPK phosphorylation to regulate osteocyte function and indicate that multiple receptors mediate rapid E(2) responses. PMID:22392527

  6. Occurrence of Actinobacillus actinomycetemcomitans in patients with chronic periodontitis, aggressive periodontitis, healthy subjects and children with gingivitis in two cities of the state of São Paulo, Brazil Ocorrência de Actinobacillus actinomycetemcomitans em pacientes com periodontite crônica, periodontite agressiva, pessoas saudáveis e crianças com gengivite em duas cidades do Estado de São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Elerson Gaetti Jardim Júnior

    2006-06-01

    Full Text Available The aim of this study was to determine the frequency of isolation of Actinobacillus actinomycetemcomitans (Aa in 100 patients with chronic periodontitis, 14 patients with aggressive periodontitis, 142 pre-school children with gingivitis and 134 periodontally healthy subjects. Samples of subgingival plaque were taken using sterilized paper points introduced into periodontal pockets or gingival crevice for 60 seconds and inoculated on TSBV agar, which was incubated under anaerobiosis at 37ºC, for 4 days. Microbial identification was performed through biochemical methods and morphocellular and morphocolonial analysis. Aa was detected in 40.3% of healthy subjects, 68% of patients with chronic periodontitis, 92.86% of patients with aggressive periodontitis and 40.14% of children with gingivitis. The rate of recovery of Aa in the tested human groups proved to be higher than previously reported and in agreement with participation of this facultative anaerobe as a member of native microbiota of the periodontium and its relation with aggressive and chronic periodontitis in Brazil.Avaliou-se a ocorrência de Actinobacillus actinmycetemcomitans (Aa em pacientes 100 pacientes com periodontite crônica, 14 com doença periodontal agressiva, 142 crianças com gengivite em idade pré-escolar e 134 indivíduos adultos saudáveis. Amostras de placa subgengival foram coletadas usando cones de papel estéreis introduzidos nas bolsas periodontais ou no sulco gengival por 60 segundos e inoculadas em ágar TSBV, que foram incubadas em anaerobiose a 37ºC, por 4 dias. A identificação microbiana foi realizada através de análises bioquímicas, morfocelulares e morfocoloniais. Aa foi detectado em 40,3% de indivíduos saudáveis, 68% de pacientes com periodontite crônica, 92,86% de pacientes com periodontite agressiva e 40,14% das crianças com gengivite. A taxa de ocorrência de Aa nos grupos testados provou ser mais alta do que a previamente descrita na literatura

  7. 二维回转培养对MLO-Y4骨样细胞PKD2表达定位及胞内钙信号的影响%The Effects of 2D-Clinorotation on Expression and Location of PKD2 Protein and the Intracellular Ca2+Concentration of MLO-Y4

    Institute of Scientific and Technical Information of China (English)

    关莹; 续惠云; 瓮媛媛; 商澎

    2014-01-01

    目的:PKD2(polycystin2,多囊肾病蛋白2)能够在细胞膜上形成无选择性的阳离子通道,在肾上皮细胞中PKD2与初级纤毛共定位,通过改变胞内的钙信号过程参与细胞对力学刺激的响应.本实验通过二维回转培养来模拟失重效应,旨在探讨二维回转培养对MLO-Y4骨样细胞PKD2表达定位,及胞内钙信号的影响.初步了解PKD2在小鼠骨样细胞MLO-Y4响应力学刺激过程中起的作用.方法:采用二维回转培养骨样细胞MLO-Y4,用RT-PCR和western blotting检测PKD2的表达,用荧光共聚焦显微镜检测细胞中PKD2与初级纤毛的定位及细胞内钙离子含量.结果:与对照组相比,在二维回转培养后,骨样细胞MLO-Y4的PKD2表达在mRNA和蛋白水平都有明显的下降,PKD2、PKD1 (polycystin1,多囊肾病蛋白1)和乙酰化的α-tubulin共定位,同时二维回转培养降低了细胞内钙离子含量.结论:在二维回转培养下,PKD2可能通过调节自身表达来改变细胞膜上PKD通道的数目和开放情况来影响细胞内钙离子含量,参与骨细胞对细胞外应力的感受过程,其详细机制还有待进一步实验研究.这将对探讨骨细胞响应力学刺激的具体机制提供重要的理论依据.

  8. 株化骨細胞MLO-Y4-A2におけるPTH受容体遺伝子のメカニカルストレスによる発現

    OpenAIRE

    岡山, 三紀; 荒川, 俊哉; 谷村, 明彦; 溝口, 到; 田隈, 泰信; オカヤマ, ミキ; アラカワ, トシヤ; タニムラ, アキヒコ; ミゾグチ, イタル; タクマ, タイシン; Miki, OKAYAMA; Toshiya, ARAKAWA; Akihiko, TANIMURA; Itaru, MIZOGUCHI; Taishin, TAKUMA

    2004-01-01

    Osteocytes are generally accepted to function as sensors for mechanical stress, which are deeply involved in bone homeostasis. However, the signal transduction mechanism of mechanical stresses in osteocytes is still mostly unclear. Here the effect of fluid shear stress on gene expressions of MLO-Y4-A2 cells, a murine osteocyte-like cell line, was investigated. When shear stress was loaded on the MLO-Y4-A2 cells, PTH receptor mRNA increased to 5 to 8 times over the control level. The PTH recep...

  9. Probiotics and periodontal health

    OpenAIRE

    Gupta, G

    2011-01-01

    Periodontitis is one of the most common chronic inflammatory diseases. The etiology is clearly bacterial and a number of putative bacterial pathogens have been associated with the disease, including Aggregatibacter actinomycetemcomitans, Tannerella forsythus and Porphyromonas gingivalis. Comparatively, little attention has been paid to the identification of health-associated and potentially beneficial bacterial species that may reside in the gingival sulcus. Probiotic technology represents a ...

  10. Association of serum immunoglobulin G (IgG) levels against two periodontal pathogens and prothrombotic state: a clinical pilot study

    OpenAIRE

    Laine Marja L; van der Velden Ubele; Nicu Elena A; Bizzarro Sergio; Loos Bruno G

    2010-01-01

    Abstract Objective Periodontitis is associated with cardiovascular diseases (CVD). In our previous studies a prothrombotic state has been observed in periodontitis, which contributes to the risk of CVD. The aim of this study was to investigate whether serum IgG levels against Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg) in periodontitis were associated with a prothrombotic state. Materials and methods Patients with moderate (n = 38) and severe periodontitis (n ...

  11. CLINICAL AND MICROBIOLOGICAL DATA IN PATIENTS WITH CHRONIC PERIODONTITIS.

    OpenAIRE

    Christina Popova; Velichka Dosseva-Panova; Vladimir E. Panov

    2013-01-01

    Periodontitis is an infectious disease concerning supporting tissues of the teeth. The primary etiological agent for disease development and progression is the subgingival biofilm, but recently it is known that host factors may modify the pathological process or may affect the severity and /or extent. The increasing levels of some specific pathogenic subgingival bacteria such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Fusobac...

  12. Antibiofilm Activity of Actinobacillus pleuropneumoniae Serotype 5 Capsular Polysaccharide

    OpenAIRE

    Karwacki, Michael T.; Kadouri, Daniel E; Meriem Bendaoud; Izano, Era A.; Vandana Sampathkumar; Inzana, Thomas J.; Kaplan, Jeffrey B.

    2013-01-01

    Cell-free extracts isolated from colony biofilms of Actinobacillus pleuropneumoniae serotype 5 were found to inhibit biofilm formation by Staphylococcus aureus, S. epidermidis and Aggregatibacter actinomycetemcomitans, but not by A. pleuropneumoniae serotype 5 itself, in a 96-well microtiter plate assay. Physical and chemical analyses indicated that the antibiofilm activity in the extract was due to high-molecular-weight polysaccharide. Extracts isolated from a mutant strain deficient in the ...

  13. Co-detección de Patógenos Periodontales en Pacientes Chilenos con Periodontitis Crónica Co-detection of Periodontal Pathogens in Chilean Patients with Chronic Periodontitis

    OpenAIRE

    C Mujica Troncoso; M Castillo-Ruiz; LK Daille; IA Fuentevilla; Bittner, M.

    2010-01-01

    Las enfermedades periodontales asociadas a la placa dental son un importante problema de salud pública. La etiología de estas patologías es de origen multifactorial e involucra factores del hospedero, medio ambiente y de carácter infeccioso asociados a bacterias embebidas en la placa dental. Las principales bacterias asociadas a la periodontitis crónica son Porphyromonas gingivalis, Treponema denticola y Tannerella forsythia, mientras que Aggregatibacter actinomycetemcomitans se ha asociado p...

  14. INFLUENCIA DE LA QUÍMICA SUPERFICIAL EN LA ENTALPÍA DE INMERSIÓN DE CARBONES ACTIVADOS EN SOLUCIONES ACUOSAS DE FENOL Y 4-NITRO FENOL

    Directory of Open Access Journals (Sweden)

    Luisa Fernanda Navarrete

    2008-04-01

    Full Text Available Se estudian las interacciones de cinco muestras de carbón activado obtenidas a partir de diferentes materiales lignocelulósicos, con diferente grado de activación alrededor de 20%, con soluciones acuosas de fenol y 4-nitro fenol mediante la determinación de las entalpías de inmersión. Se establece que los carbones activados obtenidos son de carácter básico y presentan valores para el punto de carga cero (PZC, que varían entre 7,4 y 9,7, y contenidos de basicidad total mayores en todos los casos que los valores obtenidos para la acidez total. Se determina la entalpía de inmersión de los carbones activados en soluciones de NaOH y HCl con valores mayores para la entalpía de inmersión en HCl que se encuentran entre 32,6 y 68,3 Jg-1. Las entalpías de inmersión en solución de fenol se hallan entre 7,6 y 13,9 Jg-1, y para el caso del 4-nitro fenol se encuentran entre 12,7 y 20,5 Jg-1; con valores mayores para todas las muestras para la inmersión en el segundo compuesto.

  15. 17β estradiol regulation of connexin 43-based gap junction and mechanosensitivity through classical estrogen receptor pathway in osteocyte-like MLO-Y4 cells.

    KAUST Repository

    Ren, Jian

    2013-04-01

    Connexin 43 (Cx43) plays an essential role in osteocyte mechanotransduction. Although estrogen involves in the adaptive responses of bone cells to mechanical loadings, its effects on osteocytic Cx43-based gap junction intercellular communication (GJIC) remain obscure. We found that 17β estradiol (E2) up-regulated Cx43, and enhanced GJIC in osteocyte-like MLO-Y4 cells in fluorescence recovery after photobleaching (FRAP) assay. Combination of E2 pre-treatment and oscillating fluid flow (OFF) further enhanced Cx43 expression and mitogen-activated protein kinase (MAPK) phosphorylation, comparing to E2 or OFF treatment alone. Both blocking of classical estrogen receptors (ERα/β) by fulvestrant and ERα knockdown by small interfering RNA inhibited E2-mediated Cx43 increase, while a GPR30-specific agonist G-1 failed to promote Cx43 expression. Our results suggest that the presence of E2 enhanced Cx43-based GJIC mainly via ERα/β pathway, and sensitized osteocytes to mechanical loading. © 2012 Elsevier Inc. All rights reserved.

  16. Effect of simulated microgravity and centrifugation on nitric oxide synthase activity of osteocyte-like cell line MLO-Y4

    Science.gov (United States)

    Sun, Lian-Wen; Yang, Xiao; Fan, Yu-Bo

    Bone is a highly mechanosensitive tissue, which can adapt functionally to varying levels of mechanical loads throughout a lifetime. Osteocytes are thought to be the most mechanically sensitive bone cell population. In order to understand the mechanism of microgravity-induced bone loss, it's very important to research the behavior of osteocytes under microgravity. In this study, rotary cell culture system was used to simulate microgravity. Nitric oxide synthase (NOS) activity in osteocyte-like cell MLO-Y4 was investigated under simulated microgravity. And the effect of centrifugation on NOS activity in sedentary and rotary culture cell was also investi-gated. The cultured cells were divided into four groups, including sedentary control (CON), sedentary control and centrifugation (CONC), rotary culture (RT), rotary and centrifugation (RTC). In CONC and RTC, NOS activity was determined after centrifugation (1100g 5min). The results showed NOS activity decreased significantly in RT compared with CON. However, this difference disappeared after centrifugation. On the other hand, NOS activity increased significant in RTC compared with RT while there was no difference between CON and CONC. These results indicate the normal centrifugation could counter the effect of simulated micro-gravity on NOS activity. However, it has no effect on the cells cultured under 1G. In general, osteocytes under simulated microgravity are more sensitive to centrifugation than that under 1G.

  17. Effect of simulated microgravity on nitric oxide synthase activity of osteocyte-like cell line MLO-Y4 in response to fluid shear stress

    Science.gov (United States)

    Sun, Lian-Wen; Yang, Xiao; Fan, Yu-Bo

    It is well known that microgravity could induce bone loss. However, the mechanism remains poorly understood. Osteocytes are extremely sensitive to fluid shear stress, even more than osteobleasts. The effect of simulated microgravity on osteocytes in response to fluid shear was investigated in this study in order to see if the mechanosensibility of osteocytes changed under simulated microgravity. The osteocyte-like cell line, MLO-Y4, was cultured and divided into four groups, including control (CON), control and shear (CONS), rotary (RT), rotary and shear (RTS). In RT and RTS, the cells were cultured in the rotary cell culture system to simulate microgravity condition. After 5 days, the cells in RTS and CONS were subjected to flow shear for 15 min. Then nitric oxide synthase (NOS) activity in the cells was measured using assay kit. The results showed that NOS activity in respond to fluid shear decreased significantly in RTS compared with CONS. In addition, there was significant difference in NOS activity between CONS and CON while no significant difference between RTS and RT. These indicates that the mechanosensibility of osteocytes decreased under simulated microgravity and this maybe the partly causes of the poor effect of exercise to counter microgravity-induced-bone loss. However, further research need to be done to support this finding.

  18. 17β estradiol regulation of connexin 43-based gap junction and mechanosensitivity through classical estrogen receptor pathway in osteocyte-like MLO-Y4 cells.

    Science.gov (United States)

    Ren, Jian; Wang, Xu-Hui; Wang, Guang-Chao; Wu, Jun-Hua

    2013-04-01

    Connexin 43 (Cx43) plays an essential role in osteocyte mechanotransduction. Although estrogen involves in the adaptive responses of bone cells to mechanical loadings, its effects on osteocytic Cx43-based gap junction intercellular communication (GJIC) remain obscure. We found that 17β estradiol (E2) up-regulated Cx43, and enhanced GJIC in osteocyte-like MLO-Y4 cells in fluorescence recovery after photobleaching (FRAP) assay. Combination of E2 pre-treatment and oscillating fluid flow (OFF) further enhanced Cx43 expression and mitogen-activated protein kinase (MAPK) phosphorylation, comparing to E2 or OFF treatment alone. Both blocking of classical estrogen receptors (ERα/β) by fulvestrant and ERα knockdown by small interfering RNA inhibited E2-mediated Cx43 increase, while a GPR30-specific agonist G-1 failed to promote Cx43 expression. Our results suggest that the presence of E2 enhanced Cx43-based GJIC mainly via ERα/β pathway, and sensitized osteocytes to mechanical loading. PMID:23247057

  19. Escala de medida sobre el grado de satisfacción habitacional del núcleo familiar estratos socio - económicos 3 y 4

    Directory of Open Access Journals (Sweden)

    ANDREA VÉLEZ PEREIRA

    2006-01-01

    Full Text Available La investigación de mercados no está restringida a ningún tipo específico de problema. El propósito de ésta es proporcionar información valiosa, actualizada, confiable y válida, que permita tomar las mejores decisiones al enfrentar un problema o situación especifica. En este estudio se utilizan técnicas propias de la investigación de mercados con el objetivo de identificar un proceso metodológico que permita conocer las preferencias de los clientes demandantes de vivienda y de esta forma, proporcionar una herramienta que resulte útil para el sector de la construcción ya que le brinda información valiosa para la toma de decisiones acertadas a la hora de ofrecer proyectos que logren satisfacer las necesidades de los clientes. En él se especifican y definen todos aquellos conceptos claves que hace posible el entendimiento del manual por parte del lector. Además, se ilustran algunas aplicaciones de la escala de medida del grado de satisfacción habitacional, construida por las autoras del trabajo, para los núcleos familiares en estratos socio – económicos 3 y 4 en el Valle de Aburrá, con el fin de familiarizar al usuario con la forma correcta de aplicar la escala de medida.

  20. A two-dimensional yttrium phthalate coordination polymer, [Y4(H2O)2(C8H4O4)6]∞, exhibiting different coordination geometries

    Indian Academy of Sciences (India)

    A Thirumurugan; Srinivasan Natarajan

    2003-10-01

    A hydrothermal reaction of a mixture of Y(NO3)3, 1,2-benzenedicarboxylic acid (1,2-BDC) and NaOH gives rise to a new yttrium phthalate coordination polymer, [Y4(H2O)2(C8H4O4)6]∞, I. The Y ions in I are present in four different coordination environments with respect to the oxygen atoms (CN6 = octahedral, CN7 = pentagonal bipyramid, CN8 = dodecahedron and CN9 =capped square antiprism). The oxygen atoms of the 1,2-BDC are fully deprotonated, and show variations in their connectivity with Y atoms. The Y atoms themselves are connected through their vertices forming infinite Y-O-Y one-dimensional chains. The Y-O-Y chains are cross-linked by the 1,2-BDC anions forming a corrugated layer structure. The layers are supported by favourable $\\ldots$ interactions between the benzene rings of the 1,2-BDC anions. The variations in the coordination environment of the Y atoms and the presence of Y-O-Y interactions along with the favourable $\\ldots$ interactions between the benzene rings from different layers are noteworthy structural features. Crystal data: triclinic, space group = -1 (no. 2), = 12.6669 (2), = 13.8538 (2), = 16.0289 Å, = 75.20 (1), = 69.012 (1), = 65.529 (1)°, = 2371.28 (7) Å3, calc = 1.922 g cm-1, (MoK) = 4.943 mm-1. A total of 9745 reflections collected and merged to give 6566 unique reflections (int = 0.0292) of which 5252 with > 2() were considered to be observed. Final 2 = 0.0339, 2 = 0.0724 and =1.036 were obtained for 704 parameters.

  1. Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - in vitro studies

    Science.gov (United States)

    Hayakumo, Sae; Arakawa, Shinichi; Takahashi, Masayoshi; Kondo, Keiko; Mano, Yoshihiro; Izumi, Yuichi

    2014-10-01

    The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative periodontopathogenic bacteria, Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) were evaluated using in vitro time-kill assays. The cytotoxicity of NBW3 was evaluated using three-dimensional human buccal and gingival tissue models. The numbers of colony forming units (CFUs)/mL of P. gingivalis and A. actinomycetemcomitans exposed to NBW3 dropped to below the lower limit of detection (activity against representative periodontopathogenic bacteria and is not cytotoxic to cells of human oral tissues. The use of NBW3 as an adjunct to periodontal therapy would be promising.

  2. ( Cu50Zr42Al8)96Y4块状非晶的变温晶化行为%Effects of Temperature on Crystallization Behavior of Bulk( Cu50Zr42Al8 ) 96Y4Amorphous Alloy

    Institute of Scientific and Technical Information of China (English)

    屠鹏; 寇生中

    2011-01-01

    研究(Cu50Zr42Al8)96 Y4大块非晶合金在连续升温过程中的晶化行为.结果表明,随升温速度的加快,玻璃转变温度Tg、晶化起始温度Tx、晶化峰值温度Tp都向高温区移动,过冷温度区△Tx扩大到了72.5K.运用Kissinger法分别计算出玻璃转变激活能Eg为512.34kJ/mol、晶化起始激活能Ex为372.44 kJ/mol、晶化峰的激活能Ep1和Ep1为404.52kJ/mol、404.75kJ/mol.运用FWO法计算出了晶化阶段激活能Ex,发现当晶化量小于50%时,随晶化量的增大,阶段激活能变化不大;当晶化量大于50%时,随晶化量的增大,阶段激活能呈逐渐减小的趋势.%Crystallization behavior of bulk ( Cu50 Zr42 Al8 ) 96 Y4 amorphous alloy during continuous temperature increment was described. With increasing of the temperature , The results indicate that the glass transformation temperature ( Tg ) , crystallization initial temperature ( Tz) and crystallization peak temperature ( Tp ) tend to high temperature zone , and under-cooled temperature zone △TX is enlarged to 72.5 K. The glass transformation activation energy Eg, crystallization initial activation energy Ex and crystallization peak activation energy Ep are calculated by theKissinger formula which is 512. 34 kj/mol, 372.44 kJ/mol, 404. 52 kJ/mol and 404. 75 kJ/mol respectively. In addition, crystallization phase activation energy Ex is calculated by the FWO equation. The research found that there is no notable change in the crystallization phase activation energy is increased with the increasing of crystallization degree when crystallization degree is lower than 50% ; on the contrary, when crystallization degree is higher than 50% , the crystallization phase activation energy is decreased with the increasing of crystallization degree.

  3. Activation of β–catenin Signaling in MLO-Y4 Osteocytic Cells versus 2T3 Osteoblastic Cells by Fluid Flow Shear Stress and PGE2: Implications for the Study of Mechanosensation in Bone

    OpenAIRE

    Kamel, Mohamed A; Picconi, Jason L; Lara-Castillo, Nuria; Johnson, Mark L.

    2010-01-01

    The osteocyte is hypothesized to be the mechanosensory cell in bone. However, osteoblastic cell models have been most commonly used to investigate mechanisms of mechanosensation in bone. Therefore, we sought to determine if differences might exist between osteocytic and osteoblastic cell models relative to the activation of β-catenin signaling in MLO-Y4 osteocytic, 2T3 osteoblastic and primary neonatal calvarial cells (NCCs) in response to pulsatile fluid flow shear stress (PFFSS). β–catenin ...

  4. Oscillatory fluid flow elicits changes in morphology, cytoskeleton and integrin-associated molecules in MLO-Y4 cells, but not in MC3T3-E1 cells.

    Science.gov (United States)

    Xu, Huiyun; Zhang, Jian; Wu, Jiawei; Guan, Ying; Weng, Yuanyuan; Shang, Peng

    2012-01-01

    Interstitial fluid flow stress is one of the most important mechanical stimulations of bone cells under physiological conditions. Osteocytes and osteoblasts act as primary mechanosensors within bones, and in vitro are able to respond to fluid shear stress, both morphologically and functionally. However, there is little information about the response of integrin-associated molecules using both osteoblasts and osteocytes. In this study, we investigated the changes in response to 2 hours of oscillatory fluid flow stress in the MLO-Y4 osteocyte-like cell line and the MC3T3-E1 osteoblast-like cell line. MLO-Y4 cells exhibited a significant increase in the expression of integrin-associated molecules, including OPN, CD44, vinculin and integrin αvβ3. However, there was no or limited increase observed in MC3T3-E1 osteoblast-like cells. Cell area and fiber stress formation were also markedly promoted by fluid flow only in MLO-Y4 cells. But the numbers of processes per cell remain unaffected in both cell lines. PMID:23096360

  5. Concomitant activation of the PI3K/Akt and ERK1/2 signalling is involved in cyclic compressive force-induced IL-6 secretion in MLO-Y4 cells.

    Science.gov (United States)

    Yin, Jian; Hao, Zhichao; Ma, Yuanyuan; Liao, Shuang; Li, Xianxian; Fu, Jing; Wu, Yeke; Shen, Jiefei; Zhang, Ping; Li, Xiaoyu; Wang, Hang

    2014-05-01

    IL-6 has a dual role in bone remodelling. The ERK1/2 pathway partially upregulated IL-6 secretion in osteocyte-like MLO-Y4 cells exposed to CCF. We have now investigated the possible role of phosphatidylinositol 3-kinase (PI3K)/Akt signalling pathway in the CCF-induced IL-6 expression. MLO-Y4 cells were treated with CCF 2,000 µstrain, 2 Hz, or 10, 30 min, 1, 3 and 6 h. IL-6 expression, Akt and ERK1/2 and PI3K/Akt phosphorylation were determined by RT-PCR, ELISA and Western blotting. Inhibition of PI3K/Akt with LY294002 or ERK1/2 with PD98059 significantly attenuated IL-6 upregulation, and IL-6 expression was abolished by inhibiting both pathways. Inhibition of one pathway downregulated the other's phosphorylation level. In conclusion, concomitant activation of PI3K/Akt and ERK1/2 pathways mediated IL-6 expression in MLO-Y4 cells under CCF. PMID:24375569

  6. Comparison between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in vitro%骨细胞与成骨细胞诱导破骨细胞分化的对比研究

    Institute of Scientific and Technical Information of China (English)

    崔亮; 李小彤; 杨雁琪; 傅民魁; 张丁

    2010-01-01

    目的 比较骨细胞和成骨细胞对破骨细胞分化形成的支持作用,初步探讨骨细胞在骨改建过程中的作用.方法 以小鼠骨髓基质细胞单独培养为空白对照组,以小鼠颅顶骨来源的成骨细胞与小鼠骨髓基质细胞共培养为成骨细胞组,以MLO-Y4骨细胞与小鼠骨髓基质细胞共培养为骨细胞组.使用骨吸收促进因子维生素D3处理3组细胞,抗酒石酸磷酸酶(tartrat resistant acid phosphatase,TRAP)染色后比较维生素D3处理前后3组破骨细胞数量的差异.结果 维生素D3处理前空白对照组破骨细胞计数为(6.0±1.O)个/孔板;成骨细胞组破骨细胞计数为(12.7±5.5)个/孔板,两组差异有统计学意义(P<0.05);骨细胞组破骨细胞计数为(1963.3±93.1)个/孔板,与其他两组间差异均有统计学意义(P<0.001).维生素D3对3组破骨细胞的分化形成均有促进作用.结论 在没有骨吸收促进因子存在的情况下,成骨细胞无法单独诱导破骨细胞分化,而MLO-Y4骨细胞可单独促进破骨细胞分化.骨吸收促进因子维生素D3可加强成骨细胞和骨细胞诱导破骨细胞分化的能力.%Objective To compare between MLO-Y4 osteocyte and osteoblast to support osteoclast formation in co-culture system.Methods MLO-Y4 cells and murine osteoblast cells were co-cultured with bone marrow cells with or without vitamin D3 presence.Bone marrow cells were as control group.Tartrat resistant acid phosphatase(TRAP) + giant cells with three or more nuclei were counted and compared under a microscope at day 9.Results In the absence of vitamin D3,(1963.3±93.1)/plate osteoclasts were observed when MLO-Y4 cells co-cultured with bone marrow cells in 24-well plate.While only (12.7±5.5)/plate osteoclasts were found in the osteeblast group,and (6.0±1.O)/plate in control group.The statistical difference occurs for any two groups(P <0.05).Vitamin D3 could significantly increase osteoclast formation in the three groups

  7. Descripción de la generalización de estudiantes de 3º y 4º de ESO en la resolución de problemas que involucran sucesiones lineales y cuadráticas

    OpenAIRE

    Cañadas, María C.; Castro, Encarnación; Castro, Enrique

    2008-01-01

    Describimos la generalización que logran estudiantes de 3º y 4º de Educación Secundaria Obligatoria (ESO) en la resolución de problemas que involucran sucesiones lineales y cuadráticas. La descripción se centra en aspectos relativos al razonamiento inductivo y a las estrategias inductivas. Estas estrategias permiten describir el proceso seguido en términos de los elementos y los sistemas de representación correspondientes al contenido matemático.

  8. Metabolite cross-feeding enhances virulence in a model polymicrobial infection.

    Directory of Open Access Journals (Sweden)

    Matthew M Ramsey

    2011-03-01

    Full Text Available Microbes within polymicrobial infections often display synergistic interactions resulting in enhanced pathogenesis; however, the molecular mechanisms governing these interactions are not well understood. Development of model systems that allow detailed mechanistic studies of polymicrobial synergy is a critical step towards a comprehensive understanding of these infections in vivo. In this study, we used a model polymicrobial infection including the opportunistic pathogen Aggregatibacter actinomycetemcomitans and the commensal Streptococcus gordonii to examine the importance of metabolite cross-feeding for establishing co-culture infections. Our results reveal that co-culture with S. gordonii enhances the pathogenesis of A. actinomycetemcomitans in a murine abscess model of infection. Interestingly, the ability of A. actinomycetemcomitans to utilize L-lactate as an energy source is essential for these co-culture benefits. Surprisingly, inactivation of L-lactate catabolism had no impact on mono-culture growth in vitro and in vivo suggesting that A. actinomycetemcomitans L-lactate catabolism is only critical for establishing co-culture infections. These results demonstrate that metabolite cross-feeding is critical for A. actinomycetemcomitans to persist in a polymicrobial infection with S. gordonii supporting the idea that the metabolic properties of commensal bacteria alter the course of pathogenesis in polymicrobial communities.

  9. Applied Pressure on Altering the Nano-Crystallization Behavior of Al86Ni6Y4.5Co2La1.5 Metallic Glass Powder during Spark Plasma Sintering and Its Effect on Powder Consolidation

    Directory of Open Access Journals (Sweden)

    X. P. Li

    2013-01-01

    Full Text Available Metallic glass powder of the composition Al86Ni6Y4.5Co2La1.5 was consolidated into 10 mm diameter samples by spark plasma sintering (SPS at different temperatures under an applied pressure of 200 MPa or 600 MPa. The heating rate and isothermal holding time were fixed at 40°C/min and 2 min, respectively. Fully dense bulk metallic glasses (BMGs free of particle-particle interface oxides and nano-crystallization were fabricated under 600 MPa. In contrast, residual oxides were detected at particle-particle interfaces (enriched in both Al and O when fabricated under a pressure of 200 MPa, indicating the incomplete removal of the oxide surface layers during SPS at a low pressure. Transmission electron microscopy (TEM revealed noticeable nano-crystallization of face-centered cubic (fcc Al close to such interfaces. Applying a high pressure played a key role in facilitating the removal of the oxide surface layers and therefore full densification of the Al86Ni6Y4.5Co2La1.5 metallic glass powder without nano-crystallization. It is proposed that applied high pressure, as an external force, assisted in the breakdown of surface oxide layers that enveloped the powder particles in the early stage of sintering. This, together with the electrical discharge during SPS, may have benefitted the viscous flow of metallic glasses during sintering.

  10. ESTUDIO COMPARATIVO ENTRE LAS MEDICIONES DE RUIDO AMBIENTAL URBANO A 1,5 m Y 4 m DE ALTURA SOBRE EL NIVEL DEL PISO EN LA CIUDAD DE MEDELLÍN, ANTIOQUIA - COLOMBIA

    Directory of Open Access Journals (Sweden)

    ANA JARAMILLO

    2009-01-01

    Full Text Available Con la entrada en vigencia de la Resolución 0627 de 2006, reglamentándose la altura de 4m sobre el nivel del piso como estrategia de medición para evaluar los niveles de ruido ambiental, se ha creado una gran controversia entre las entidades ambientales gubernamentales, los académicos y profesionales del sector, sobre las implicaciones económicas, logísticas y operativas para el desarrollo del trabajo de campo. Con el propósito de determinar si mediciones simultáneas de ruido urbano efectuadas a 1,5 m y 4 m representan o no la misma realidad sonora, se realizó un análisis estadístico a un conjunto de datos obtenidos en el marco de la construcción del mapa de ruido del municipio. Después de un análisis de los datos agrupados por punto, por jornada (diurna y nocturna y por tipo de día (hábil y no hábil, se infiere que no es posible afirmar la semejanza entre muestras de ruido tomadas a 1,5 m y 4 m de altura, no sólo por las diferencias numéricas, sino que representan realidades sonoras diferentes.

  11. HACEK endocarditis: state-of-the-art.

    Science.gov (United States)

    Revest, Matthieu; Egmann, Gérald; Cattoir, Vincent; Tattevin, Pierre

    2016-01-01

    The HACEK group of bacteria - Haemophilus parainfluenzae, Aggregatibacter spp. (A. actinomycetemcomitans, A. aphrophilus, A. paraphrophilus, and A. segnis), Cardiobacterium spp. (C. hominis, C. valvarum), Eikenella corrodens, and Kingella spp. (K. kingae, K. denitrificans) - are fastidious gram-negative bacteria, part of the normal microbiota of oral and upper respiratory tract in humans. Although their pathogenicity is limited, they are responsible for 1-3% of all infective endocarditis. HACEK endocarditis mostly affect patients with underlying heart disease or prosthetic valves, and are characterized by an insidious course, with a mean diagnosis delay of 1 month (Haemophilus spp.) to 3 months (Aggregatibacter and Cardiobacterium spp.). The advent of continuously monitored blood culture systems with enriched media has erased the need for extended incubation for the diagnosis of HACEK endocarditis. Medical treatment relies on third-generation cephalosporin, with a favorable outcome in 80-90% of cases, with or without cardiac surgery. PMID:26953488

  12. A utilização do gel de metronidazol numa concentração de 25% de aplicação local no tratamento periodontal não cirúrgico

    OpenAIRE

    Costa, Sandro Maciel Lopes da

    2016-01-01

    A doença periodontal continua a ser uma das doenças inflamatórias crónicas, com elevados níveis de prevalência por todo o mundo, sendo a principal causa de perda de dentes na idade adulta. Classificada como uma doença infeciosa e de carácter multifatorial, onde estirpes bacterianas como: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis e Prevotella intermedia são o seu principal fator etiológico, devido ao expressarem fortes fatores de virulência e induzirem a um...

  13. Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria.

    Science.gov (United States)

    Fani, Mohammadmehdi; Kohanteb, Jamshid

    2012-03-01

    Aloe vera is a medicinal plant with anti-inflammatory, antimicrobial, antidiabetic and immune-boosting properties. In the present study we investigated the inhibitory activities of Aloe vera gel on some cariogenic (Streptococcus mutans), periodontopathic (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis) and an opportunistic periodontopathogen (Bacteroides fragilis) isolated from patients with dental caries and periodontal diseases. Twenty isolates of each of these bacteria were investigated for their sensitivity to Aloe vera gel using the disk diffusion and microdilution methods. S. mutans was the species most sensitive to Aloe vera gel with a MIC of 12.5 µg/ml, while A. actinomycetemcomitans, P. gingivalis, and B. fragilis were less sensitive, with a MIC of 25-50 µg/ml (P Aloe vera gel at optimum concentration could be used as an antiseptic for prevention of dental caries and periodontal diseases.

  14. Periodontal Pathogens and Atherosclerosis: Implications of Inflammation and Oxidative Modification of LDL

    Directory of Open Access Journals (Sweden)

    Tomoko Kurita-Ochiai

    2014-01-01

    Full Text Available Inflammation is well accepted to play a crucial role in the development of atherosclerotic lesions, and recent studies have demonstrated an association between periodontal disease and cardiovascular disease. Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, causative agents of destructive chronic inflammation in the periodontium, can accelerate atheroma deposition in animal models. Emerging evidence suggests that vaccination against virulence factors of these pathogens and anti-inflammatory therapy may confer disease resistance. In this review, we focus on the role of inflammatory mechanisms and oxidative modification in the formation and activation of atherosclerotic plaques accelerated by P. gingivalis or A. actinomycetemcomitans in an ApoE-deficient mouse model and high-fat-diet-fed mice. Furthermore, we examine whether mucosal vaccination with a periodontal pathogen or the anti-inflammatory activity of catechins can reduce periodontal pathogen-accelerated atherosclerosis.

  15. 伴放线放线杆菌cdtB基因克隆及其表达蛋白的体外生物学活性检测%Gene cloning of cdtB from Actinobacillus actinomycetemcomitans and bioactivity test of the recombinant protein CdtB in vitro

    Institute of Scientific and Technical Information of China (English)

    李璐; 段君兰; 王晓茜; 杨迷芳; 徐艳

    2010-01-01

    Objective To construct the prokayotic expression vector pET-15b-cdtB containing the cdtB gene from Actinobacillus actinomycetemcomitans (Aa) and to test the bioactivity of this recombinant CdtB in vitro. Methods The toxic cytolethal distending toxin (CDT) subunit encoding gene cdtB was amplified by PCR. Through restriction endonuclease digestion,gene cdtB and vector pET-15b were ligated to form pET-15b-cdtB expression system which was transformed into competent cells Escherichia coli BL21 ( DE3 ). Protein expression was induced by isopropyl-beta-D-thiogalactoside and examined by sodium dodecyl sulfate-pelyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Supercoiled plasmid pET-32a DNA was incubated with purified recombinant CdtB protein in vitro to view any changes in the electrophoretic mobility of the plasmid pET-32a DNA band. Results PCR testing results of pET-15b-cdtB transformed cells demonstrated that all strains contained cdtB gene. The DNA sequence was blast with cdtB gene from GenBank and 99% homology was obtained. Both of SDS-PAGE and Western blotting confirmed that recombinant CdtB was obtained. After incubated with the purified recombinant CdtB in vitro, the supercoiled plasmid pET-32a DNA was observed relaxing by 1% ngarose gel electrophoresis test. Conclusions The recombinant plasmid pET-15b-cdtB was successfully constructed and the recombinant CdtB protein which has the Dnase Ⅰ -like activity was obtained.%目的 体外构建伴放线放线杆菌(Actinobacillus actinomycetemcomitans,Aa)CdtB蛋白的原核表达载体并诱导其表达,通过变性、复性获得有Ⅰ型脱氧核糖核酸酶(deoxyribonuclease Ⅰ,DNase Ⅰ)样活性的重组CdtB蛋白,为进一步研究AaCdtB的功能以及Aa细胞致死性扩张毒素三聚体全毒素在牙周炎发生、发展过程中的分子致病机制奠定基础.方法 以AaATCC29522基因组DNA为模板,采用聚合酶链反应(PCR)法获得cdtB基因,经双酶切、连接的定向克隆

  16. Synthesis and luminescence characteristics of Li2Y4-xEux(WO4)7-y(MoO4)y red-emitting phosphor for white LED

    Institute of Scientific and Technical Information of China (English)

    茹晶晶; 郭飞云; 陈建中

    2013-01-01

    Li2Y4-xEux(WO4)7-y(MoO4)y red-emitting phosphors were synthesized by solid state reaction and characterized by powder X-ray diffraction (XRD) and photoluminescence (PL) spectrum. The excitation spectra showed that the phosphors could be efficiently excited by near-UV light of 395 nm. When the relative molar ratio of Mo/W was 7:0, and the optimum doped concentration of Eu3+was 2.8 mol, the phosphor showed strong red emission lines at 615 nm corresponding to the forced electric dipole 5D0→7F2 transition of Eu3+. Compared with Na2Y2Eu2(MoO4)7 and K2Y2Eu2(MoO4)7, the fluorescence intensity of Li2Y1.2Eu2.8(MoO4)7 phosphor was the strongest. The CIE chromaticity coordinates of Li2Y1.2Eu2.8(MoO4)7 phosphor was calculated to be (0.66, 0.34).

  17. Essential Oils from Ugandan Aromatic Medicinal Plants: Chemical Composition and Growth Inhibitory Effects on Oral Pathogens

    Directory of Open Access Journals (Sweden)

    Francis Ocheng

    2015-01-01

    Full Text Available The study assessed the growth inhibitory effects of essential oils extracted from ten Ugandan medicinal plants (Bidens pilosa, Helichrysum odoratissimum, Vernonia amygdalina, Hoslundia opposita, Ocimum gratissimum, Cymbopogon citratus, Cymbopogon nardus, Teclea nobilis, Zanthoxylum chalybeum, and Lantana trifolia used traditionally in the management of oral diseases against oral pathogens. Chemical compositions of the oils were explored by GC-MS. Inhibitory effects of the oils were assessed on periodontopathic Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans and cariogenic Streptococcus mutans and Lactobacillus acidophilus using broth dilution methods at concentrations of 1%, 0.1%, and 0.01%. The most sensitive organism was A. actinomycetemcomitans. Its growth was markedly inhibited by six of the oils at all the concentrations tested. Essential oil from C. nardus exhibited the highest activity with complete growth inhibition of A. actinomycetemcomitans and P. gingivalis at all the three concentrations tested, the major constituents in the oil being mainly oxygenated sesquiterpenes. Most of the oils exhibited limited effects on L. acidophilus. We conclude that essential oils from the studied plants show marked growth inhibitory effects on periodontopathic A. actinomycetemcomitans and P. gingivalis, moderate effects on cariogenic S. mutans, and the least effect on L. acidophilus. The present study constitutes a basis for further investigations and development of certain oils into alternative antiplaque agents.

  18. Effects of high glucose and advanced glycation end products on the expressions of sclerostin and RANKL as well as apoptosis in osteocyte-like MLO-Y4-A2 cells

    Energy Technology Data Exchange (ETDEWEB)

    Tanaka, Ken-ichiro, E-mail: ken1nai@med.shimane-u.ac.jp; Yamaguchi, Toru, E-mail: yamaguch@med.shimane-u.ac.jp; Kanazawa, Ippei, E-mail: ippei.k@med.shimane-u.ac.jp; Sugimoto, Toshitsugu, E-mail: sugimoto@med.shimane-u.ac.jp

    2015-05-29

    In diabetes mellitus (DM), high glucose (HG) and advanced glycation end products (AGEs) are involved in bone quality deterioration. Osteocytes produce sclerostin and receptor activator of nuclear factor-kB ligand (RANKL) and regulate osteoblast and osteoclast function. However, whether HG or AGEs directly affect osteocytes and regulate sclerostin and RANKL production is unknown. Here, we examined the effects of HG, AGE2, and AGE3 on the expression of sclerostin and RANKL and on apoptosis in osteocyte-like MLO-Y4-A2 cells. Treatment of the cells with 22 mM glucose, 100 μg/mL either AGE2 or AGE3 significantly increased the expression of sclerostin protein and mRNA; however, both AGEs, but not glucose, significantly decreased the expression of RANKL protein and mRNA. Moreover, treatment of the cells with HG, AGE2, or AGE3 for 72 h induced significant apoptosis. These detrimental effects of HG, AGE2, and AGE3 on sclerostin and RANKL expressions and on apoptosis were antagonized by pretreatment of the cells with 10{sup −8} M human parathyroid hormone (PTH)-(1–34). Thus, HG and AGEs likely suppress bone formation by increasing sclerostin expression in osteocytes, whereas AGEs suppress bone resorption by decreasing RANKL expression. Together, these processes may cause low bone turnover in DM. In addition, HG and AGEs may cause cortical bone deterioration by inducing osteocyte apoptosis. PTH may effectively treat these pathological processes and improve osteocyte function. - Highlights: • AGEs are involved in bone quality deterioration in diabetes mellitus (DM). • AGEs increased sclerostin as well as apoptosis, and decreased RANKL in osteocytes. • The effects of AGEs on osteocyte function were antagonized by human PTH-(1–34). • AGEs may cause low bone turnover and cortical porosity in DM. • PTH may be effective in bone quality deterioration by improving osteocyte function.

  19. Effects of high glucose and advanced glycation end products on the expressions of sclerostin and RANKL as well as apoptosis in osteocyte-like MLO-Y4-A2 cells.

    Science.gov (United States)

    Tanaka, Ken-ichiro; Yamaguchi, Toru; Kanazawa, Ippei; Sugimoto, Toshitsugu

    2015-05-29

    In diabetes mellitus (DM), high glucose (HG) and advanced glycation end products (AGEs) are involved in bone quality deterioration. Osteocytes produce sclerostin and receptor activator of nuclear factor-кB ligand (RANKL) and regulate osteoblast and osteoclast function. However, whether HG or AGEs directly affect osteocytes and regulate sclerostin and RANKL production is unknown. Here, we examined the effects of HG, AGE2, and AGE3 on the expression of sclerostin and RANKL and on apoptosis in osteocyte-like MLO-Y4-A2 cells. Treatment of the cells with 22 mM glucose, 100 μg/mL either AGE2 or AGE3 significantly increased the expression of sclerostin protein and mRNA; however, both AGEs, but not glucose, significantly decreased the expression of RANKL protein and mRNA. Moreover, treatment of the cells with HG, AGE2, or AGE3 for 72 h induced significant apoptosis. These detrimental effects of HG, AGE2, and AGE3 on sclerostin and RANKL expressions and on apoptosis were antagonized by pretreatment of the cells with 10(-8) M human parathyroid hormone (PTH)-(1-34). Thus, HG and AGEs likely suppress bone formation by increasing sclerostin expression in osteocytes, whereas AGEs suppress bone resorption by decreasing RANKL expression. Together, these processes may cause low bone turnover in DM. In addition, HG and AGEs may cause cortical bone deterioration by inducing osteocyte apoptosis. PTH may effectively treat these pathological processes and improve osteocyte function. PMID:25721666

  20. ¿Una teología del martirio en 1QHª y 4Q491c?: Aportes para la comprensión de la cristología del Hijo del hombre joánico ¿A Theology of martyrdom in 1QHª y 4Q491c?: Contributions to the understanding of the Chistology of the Johannine Son of man

    Directory of Open Access Journals (Sweden)

    César Carbullanca Núñez

    2011-09-01

    Full Text Available El artículo pretende poner en duda las explicaciones acerca de la existencia de una cristología de la exaltación o una dependencia de los dichos sinópticos sobre el Hijo del hombre y además pretende dar antecedentes sobre la vinculación entre exaltación y muerte en el texto de los 1QHa y 4Q491c presente en la literatura de Qumrán, lo que arroja luz acerca de la existencia de una teología del martirio que se desarrolló en tiempos pre-cristianos que integraba tanto los aspectos de persecución y sufrimiento en el servicio a Dios como el de una posterior exaltación. El artículo analiza los textos 1QHa y 4Q491c mostrando que la secuencia sufrimiento-exaltación a los cielos, el uso del término «exaltación» y «glorificación», ya se encuentran antes de su uso cristiano. Estas pruebas demuestran lo arbitrario de algunas teorías que pretenden dividir los relatos de la pasión joánico sosteniendo que habría existido una «cristología de la exaltación» independiente del relato de la muerte del Hijo del hombre y postula, por consiguiente, la necesidad de una visión más integral de la cristología joánica.The This article attempts to cast doubt on explanations for the existence of a Christology of exaltation or reliance on such a Synoptics on the Son of man and therefore seeks to give a prior history of the links between exaltation and death in the text of the 1QHa and 4Q491c present in the Qumrán lüerature, which sheds light on the existence of a theology of martyrdom that took place in pre-Christian times that integrates aspects of persecution and suffering in the service of God as the subsequent exaltation. The article analyzes texts 1QHa and 4Q491c sequence showing suffering exaltation to heaven, the term «exaltation» and «glorification» are already Christian before use These tests demónstrate the arbitrariness of some theories that try to divide the accounts of thejohannine passion arguing that there had been a

  1. Fluoroquinolones in the treatment of Actinobacillus actinomycetemcomitans associated periodontitis

    NARCIS (Netherlands)

    Kleinfelder, JW; Mueller, RF; Lange, DE

    2000-01-01

    Background: Periodontitis patients harboring Actinobacillus actinmycetemcomitans (Aa) are prime candidates for systemic antibiotic therapy. Besides tetracycline and the combination of metronidazole and amoxicillin the fluoroquinolones are also believed to have antibacterial activity against Aa. The

  2. Introducción de una metodología basada en la utilización de agrupamientos flexibles multiniveles para la resolución de problemas matemáticos en 3º y 4º de la ESO

    OpenAIRE

    Revilla-Manrique, Aintzane

    2016-01-01

    Mediante esta investigación, se ha diseñado una propuesta basada en agrupamientos flexibles multiniveles como medida de atención a la diversidad en el ámbito de las matemáticas, más concretamente para la resolución de problemas. El estudio se ha basado en los cursos de 3º y 4º de la ESO por ser los últimos pertenecientes a la educación obligatoria y por ello, una etapa clave para la elección de los estudios superiores. Como base del trabajo, se presenta un marco teórico que eng...

  3. Oxygen as a Virulence Determinant in Polymicrobial Infections.

    Science.gov (United States)

    Selleck, Elizabeth M; Gilmore, Michael S

    2016-01-01

    Infections caused by multiple organisms, or polymicrobial infections, are likely more common than is broadly appreciated. Interaction among microbial communities (and with their host) can change the infection landscape by subverting immunity, providing nutrients and inhibiting competing microbes. Stacy et al. (A. Stacy, D. Fleming, R. J. Lamont, K. P. Rumbaugh, and M. Whiteley, mBio 7:e00782-16, 2016, http://dx.doi.org/10.1128/mBio.00782-16) described a novel mechanism that results in synergistic growth of oral microbes Aggregatibacter actinomycetemcomitans and Streptococcus gordonii The authors used whole-genome fitness profiling by transposon sequencing (Tn-seq) to identify genes differentially required for growth in vitro versus in a mono- or coinfection in a thigh abscess model. They found that coinfection with S. gordonii allowed A. actinomycetemcomitans to shift from an anaerobic to an aerobic mode of growth. This shift involved the production of a terminal electron acceptor H2O2 by S. gordonii and increased A. actinomycetemcomitans persistence-an interaction termed "cross-respiration." PMID:27531913

  4. Co-detección de Patógenos Periodontales en Pacientes Chilenos con Periodontitis Crónica Co-detection of Periodontal Pathogens in Chilean Patients with Chronic Periodontitis

    Directory of Open Access Journals (Sweden)

    C Mujica Troncoso

    2010-12-01

    Full Text Available Las enfermedades periodontales asociadas a la placa dental son un importante problema de salud pública. La etiología de estas patologías es de origen multifactorial e involucra factores del hospedero, medio ambiente y de carácter infeccioso asociados a bacterias embebidas en la placa dental. Las principales bacterias asociadas a la periodontitis crónica son Porphyromonas gingivalis, Treponema denticola y Tannerella forsythia, mientras que Aggregatibacter actinomycetemcomitans se ha asociado principalmente a la periodontitis agresiva. Otro microorganismo clave en el desarrollo de la enfermedad es Fusobacterium nucleatum, el cual tiene la capacidad de co-agregarse con los patógenos periodontales y así facilitar su colonización. Para demostrar la prevalencia de estas bacterias, la co-detección y la asociación entre pacientes chilenos fumadores y no fumadores, se analizaron 67 muestras mediante PCR convencional. Los resultados mostraron que un 90% de las muestras fueron positivas para F. nucleatum, siendo la bacteria más detectada. Al analizar la co-detección entre las distintas bacterias se observa que F nucleatum está presente en más de un 80% de los casos cuando se detecta cualquiera de las cuatro bacterias restantes, mientas que A. actinomycetemcomitans se detecta en no más de un 20% al amplificar cualquiera de las bacterias restantes. Por otra parte, los resultados por género indican que existen diferencias significativas en la detección de T. forsythia, F. nucleatum y A. actinomycetemcomitans. Al considerar el factor de tabaquismo se observó que ninguna de las muestras de pacientes fumadores resultó ser positiva para A. actinomycetemcomitans.Periodontal disease associated with dental plaque is a major public health problem. The etiology of these disorders is multifactorial involved seeing host factors, environmental factors and infectious nature associated with the presence of bacteria belonging to the plaque. The main

  5. Antimicrobial activity of diterpenes from Viguiera arenaria against endodontic bacteria.

    Science.gov (United States)

    Carvalho, Tatiane C; Simão, Marília R; Ambrósio, Sérgio R; Furtado, Niege A J C; Veneziani, Rodrigo C S; Heleno, Vladimir C G; Da Costa, Fernando B; Gomes, Brenda P F A; Souza, Maria Gorete M; Borges dos Reis, Erika; Martins, Carlos H G

    2011-01-01

    Six pimarane-type diterpenes isolated from Viguiera arenaria Baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. The microdilution method was used for the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Porphyromonas gingivalis, Prevotella nigrescens, Prevotella intermedia, Prevotella buccae, Fusobacterium nucleatum, Bacteroides fragilis, Actinomyces naeslundii, Actinomyces viscosus, Peptostreptococcus micros, Enterococcus faecalis and Aggregatibacter actinomycetemcomitans. The compounds ent-pimara-8(14),15-dien-19-oic acid, its sodium salt and ent-8(14),15-pimaradien-3β-ol were the most active, displaying MIC values ranging from 1 to 10 μg mL-1. The results also allow us to conclude that minor structural differences among these diterpenes significantly influence their antimicrobial activity, bringing new perspectives to the discovery of new chemicals for use as a complement to instrumental endodontic procedures. PMID:21233793

  6. Antimicrobial Activity of Protamine against Oral Microorganisms.

    Science.gov (United States)

    Kim, Yeon-Hee; Kim, Sang Moo; Lee, Si Young

    2015-01-01

    Protamine is an arginine-rich polycationic protein extracted from sperm cells of vertebrates including fishes such as salmon. The purpose of this study was to investigate the suppressive effects of protamine on the growth of oral pathogens for possible usage in dental materials. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined by the microdilution method. Twelve strains of oral viridans streptococci, Actinomyces naeslundii, Actinomyces odontolyticus, Enterococcus faecalis, Lactobacillus acidophilus, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis and Candida albicans were suppressed by protamine. MIC and MBC values were between 0.009 ~ 20 mg/mL and 0.019 ~ 80 mg/mL, respectively. The bactericidal activities of protamine against susceptible bacterial species were dependent on the concentration of protamine and incubation time. Based on the results of this study, protamine would be a useful compound for the development of antimicrobial agents against oral pathogens in dental materials. PMID:26699859

  7. Antimicrobial Activity of Diterpenes from Viguiera arenaria against Endodontic Bacteria

    Directory of Open Access Journals (Sweden)

    Carlos H. G. Martins

    2011-01-01

    Full Text Available Six pimarane-type diterpenes isolated from Viguiera arenaria Baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. The microdilution method was used for the determination of the minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC against Porphyromonas gingivalis, Prevotella nigrescens, Prevotella intermedia, Prevotella buccae, Fusobacterium nucleatum, Bacteroides fragilis, Actinomyces naeslundii, Actinomyces viscosus, Peptostreptococcus micros, Enterococcus faecalis and Aggregatibacter actinomycetemcomitans. The compounds ent-pimara-8(14,15-dien-19-oic acid, its sodium salt and ent-8(14,15-pimaradien-3β-ol were the most active, displaying MIC values ranging from 1 to 10 μg mL-1. The results also allow us to conclude that minor structural differences among these diterpenes significantly influence their antimicrobial activity, bringing new perspectives to the discovery of new chemicals for use as a complement to instrumental endodontic procedures.

  8. ASSOCIATION OF THE BONE LOSS WITH MAIN CLINICAL AND MICROBIOLOGICAL PARAMETERS IN CHRONIC PERIODONTITIS

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    Velitchka Dosseva-Panova

    2014-07-01

    Full Text Available Literature data have reported that certain Gram-negative anaerobe bacteria from the subgingival environment are important etiological agents of chronic periodontal disease. Based on published criteria identifying periodontal pathogens, major evidences has been shown for: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythia; moderate evidences for: Prevotella intermedia, Peptostreptococcus /Micromonas/ micros, Fusobacterium nucleatum, Eubacterium nodatum, Campylobacter rectus, and spirochetes (Treponema denticola. Microbiological data has been suggested by several studies as a useful indicator of disease progression, selection of an adjunctive antimicrobial administration, healing assessment and disease recurrence. The results of this study demonstrate statistically significant correlation between periodontal attachment loss and the prevalence of Porphyromonas gingivalis, Tannerella forsythia and Peptostreptococcus (Micromonas micros in deep periodontal pockets of severe chronic periodontitis patients.

  9. Synthesis of new carolacton derivatives and their activity against biofilms of oral bacteria.

    Science.gov (United States)

    Stumpp, N; Premnath, P; Schmidt, T; Ammermann, J; Dräger, G; Reck, M; Jansen, R; Stiesch, M; Wagner-Döbler, I; Kirschning, A

    2015-05-28

    Carolacton, a secondary metabolite isolated from the extracts of Sorangium cellulosum, causes membrane damage and cell death in biofilms of the caries- and endocarditis-associated bacterium Streptococcus mutans. Here, we report the total synthesis of several derivatives of carolacton. All new structural modifications introduced abolished its biological activity, including subtle ones, such as inversion of configuration at C9. However, a bicyclic bislactone derivative as well as the methyl ester of carolacton resulted in compounds with prodrug properties. Their inhibitory activity on S. mutans was proven to be based on enzymatic hydrolysis by S. mutans which provided native carolacton resulting in biofilm damage in vivo. Moreover, we demonstrate that carolacton acts also on S. gordonii, S. oralis and the periodontitis pathogen Aggregatibacter actinomycetemcomitans, causing elongated cells and growth inhibition.

  10. CLINICAL AND MICROBIOLOGICAL DATA IN PATIENTS WITH CHRONIC PERIODONTITIS.

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    Christina Popova

    2013-07-01

    Full Text Available Periodontitis is an infectious disease concerning supporting tissues of the teeth. The primary etiological agent for disease development and progression is the subgingival biofilm, but recently it is known that host factors may modify the pathological process or may affect the severity and /or extent. The increasing levels of some specific pathogenic subgingival bacteria such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Fusobacterium nucleatum, Prevotella intermedia and others can result in periodontal destruction and possibly correlate with disease severity. Data from controlled studies show high prevalence of P. gingivalis, T. forsythia and Tr. denticola which represent the red complex (coexistence of these three species in patients with moderate and severe chronic periodontitis. Parallel investigation of probing depth (PD and clinical attachment level (CAL with the microbiological testing may give a confirmation of relation between subgingival pathogenic bacteria and severity of periodontitis.

  11. Nonsyndromic localized aggressive periodontitis of primary dentition: A rare case report

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    Radhika Muppa

    2016-01-01

    Full Text Available Periodontitis is an inflammatory disease of the gingiva and tissues of the periodontium. It is characterized by pocket formation and destruction of supporting alveolar bone. Periodontal diseases of aggressive nature are not very common in children. They are usually associated with systemic conditions. The present case report is of a 5-year-old male child who reported with rapid attachment loss and bony defects of the gingiva and supporting structures. His family and medical history gave no contribution for the diagnosis. Blood investigations did not reveal any abnormality. The microbial examination of culture revealed the presence of periodontal pathogen Aggregatibacter actinomycetemcomitans. The treatment objective in the present case was to prevent the further progress of the condition, restore esthetic and function in the child which would psychologically benefit the child.

  12. Mediadores bioquímicos involucrados en la fisiopatología y diagnóstico de la enfermedad periodontal

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    Facundo Ledesma

    2012-09-01

    Full Text Available En el desarrollo de la enfermedad periodontal  (EP  intervienen enzimas derivadas tanto del huésped como de los microorganismos presentes en la placa dental.  La presente revisión abordó el papel que cumplen diversos mediadores de la EP y sus utilidades clínicas. La fosfatasa alcalina, la β-glucuronidasa, la catepsina B, las MMP-8 y MMP-9, la elastasa y las Dipeptidil peptidasas II y IV, son útiles en el monitoreo y la planificación del tratamiento. La catepsina B, la MMP-8, la elastasa, las dipeptidil peptidasas II y IV, son utilizadas para diferenciar la gingivitis y la periodontitis. Las exotoxinas bacterianas del Aggregatibacter  actinomycetemcomitans, indican la severidad de la enfermedad periodontal agresiva y ayudan al pronóstico del caso.

  13. Patrones, Generalización y Estrategias Inductivas de Estudiantes de 3º y 4º de Educación Secundaria Obligatoria en el Problema de las Baldosas (Patterns, Generalization and Inductive Strategies of Secondary Students Working on the Tiles Problem

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    María C. Cañadas

    2008-01-01

    Full Text Available En este trabajo describimos los patrones y la generalización que llevan a cabo 359 estudiantes de 3º y 4º de Educación Secundaria Obligatoria en la resolución del problema de las baldosas. Prestamos especial atención a los tipos de patrones identificados, a la forma en que los estudiantes expresan la generalización y, mediante la descripción de las estrategias inductivas, presentamos algunas características de la generalización referentes a los elementos y a los sistemas de representación utilizados. In this paper we explore the patterns and the generalization developed by 359 students in years 9 and 10 in the resolution of the tiles problem. We pay special attention to the kinds of patterns identified, to the written ways in which students express generalization and, using inductive strategies, we present some characteristics of the generalization relating to the elements and the representations used.

  14. The in Vitro Antimicrobial Efficacy of PDT against Periodontopathogenic Bacteria

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    Philippe A. Haag

    2015-11-01

    Full Text Available Periodontitis, an inflammatory disease, is caused by biofilms with a mixed microbial etiology and involves the progressive destruction of the tooth-supporting tissues. A rising number of studies investigate the clinical potential of photodynamic therapy (PDT as an adjunct during active therapy. The aim of the present review was to evaluate the available literature for the in vitro antimicrobial efficacy of photodynamic therapy focusing on the periodontopathogenic bacteria Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Fusobacterium nucleatum. The focused question was: “Is it possible to decrease (at least 3 log steps or 99.9% or even eliminate bacterial growth by photodynamic therapy in vitro when compared to untreated control groups or control groups treated by placebo?” In general, PDT resulted in a substantial reduction of surviving bacteria. However, not all studies showed the desired reduction or elimination. The ranges of log10-reduction were 0.38 (58% to a complete eradication (100% for P. gingivalis, 0.21 (39% to 100% for A. actinomycetemcomitans and 0.3 (50% to 100% for F. nucleatum. In conclusion, further and particularly more comparable studies are needed to evaluate if PDT can be clinically successful as an adjuvant in periodontal therapy.

  15. Effectiveness of ozone against periodontal pathogenic microorganisms.

    Science.gov (United States)

    Huth, Karin C; Quirling, Martina; Lenzke, Stefanie; Paschos, Ekaterini; Kamereck, Klaus; Brand, Korbinian; Hickel, Reinhard; Ilie, Nicoleta

    2011-06-01

    Ozone has been proposed as an adjunct antiseptic in periodontitis therapy. The aim of this study was to investigate the antimicrobial effectiveness of gaseous/aqueous ozone, in comparison with that of the established antiseptic chlorhexidine digluconate (CHX), against periodontal microorganisms. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Parvimonas micra in planktonic or biofilm cultures were exposed, for 1 min, to gaseous ozone, aqueous ozone, CHX, or phosphate-buffered saline (control). None of the agents was able to substantially reduce the A. actinomycetemcomitans count in biofilm cultures. In contrast, P. gingivalis, T. forsythia, and P. micra could be eliminated by 2% CHX or by ozone gas at 53 gm(-3) . Significantly greater antimicrobial effects were observed against planktonic cultures than against biofilm-associated bacteria. The rate of killing was influenced by the species of bacteria, and by the type and concentration of agent. There were no significant differences in the effectiveness of aqueous ozone (20 μg ml(-1) ) or gaseous ozone (≥ 4 gm(-3) ) compared with 2% CHX but they were more effective than 0.2% CHX. Therefore, high-concentrated gaseous and aqueous ozone merit further investigation as antiseptics in periodontitis therapy. A safe system for applying gaseous ozone into the periodontal pocket that avoids inhalation still needs to be developed.

  16. Low levels of caries in aggressive periodontitis: A literature review

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    Sulugodu Ramachandra Srinivas

    2014-04-01

    Full Text Available This article is a traditional literature review on caries levels in aggressive periodontitis. Aggressive periodontitis generally affects systemically healthy individuals aged <30 years (older individuals can also be affected and is characterized by a young age of onset, rapid rate of disease progression, and familial aggregation of cases. Dental caries is caused by the dissolution of enamel by acid-producing bacteria present in the plaque biofilm, especially when the biofilm reaches critical mass due to improper oral hygiene. The association between caries level and aggressive periodontitis has long been debated. Initial research indicated that caries levels were high in patients with aggressive periodontitis, but high-quality studies have consistently shown that caries and aggressive periodontitis are inversely related. A recent in vitro study showed that Streptococcus mutans was killed more readily in the saliva of patients with aggressive periodontitis and Aggregatibacter actinomycetemcomitans positivity than in patients with A. actinomycetemcomitans negativity. Other mechanisms possibly explaining the inverse relationship between caries and aggressive periodontitis in cases of Down’s syndrome are also discussed in this literature review. The usefulness of caries level in the diagnosis of aggressive periodontitis in developing countries such as India, where the disease is diagnosed primarily on the basis of clinical and radiographic features and familial history is also discussed.

  17. Prevalence of periodontopathogens and Candida spp. in smokers after nonsurgical periodontal therapy - a pilot study.

    Science.gov (United States)

    Camargo, Gabriela Alessandra da Cruz Galhardo; Abreu, Mariana Gouvêa Latini; Cordeiro, Renata Dos Santos; Wenderoscky, Letícia de Farias; Duque, Cristiane

    2016-01-01

    This pilot study aimed to evaluate the influence of smoking on clinical and microbiological parameters after nonsurgical periodontal therapy. Forty-eight subjects were grouped into smokers (SM, n = 24) and nonsmokers (NS, n = 24) and paired according to gender, age, ethnicity, and periodontal status. Both groups received oral hygiene education and scaling and root planing. Clinical evaluation was performed using plaque index (PI), bleeding on probing (BOP), pocket probing depth (PPD), gingival recession (GR), and clinical attachment level (CAL) before instrumentation (baseline) and at 3 and 6 months. The prevalence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Candida albicans, Candida glabrata, Candida tropicalis, and Candida dubliniensis in subgingival biofilm was determined by polymerase chain reaction. The data were statistically analyzed considering p CAL increase in comparison to SM. Periodontal treatment reduced the levels of P. gingivalis, A. actinomycetemcomitans, and T. forsythia individually after 3 months for the NS group and after 6 months for both groups. The prevalence of Candida species was markedly higher in SM than in NS at all time points evaluated. Periodontopathogens associated or not with C. albicans or C. dubliniensis were more prevalent in SM than in NS at baseline and after 3 months. It was concluded that smoking impairs clinical and microbiological responses to periodontal therapy. Periodontopathogens combined or not with some Candida species are resistant to short-term periodontal therapy in SM. PMID:27556680

  18. Nanoparticle-encapsulated chlorhexidine against oral bacterial biofilms.

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    Chaminda Jayampath Seneviratne

    Full Text Available BACKGROUND: Chlorhexidine (CHX is a widely used antimicrobial agent in dentistry. Herein, we report the synthesis of a novel mesoporous silica nanoparticle-encapsulated pure CHX (Nano-CHX, and its mechanical profile and antimicrobial properties against oral biofilms. METHODOLOGY/PRINCIPAL FINDINGS: The release of CHX from the Nano-CHX was characterized by UV/visible absorption spectroscopy. The antimicrobial properties of Nano-CHX were evaluated in both planktonic and biofilm modes of representative oral pathogenic bacteria. The Nano-CHX demonstrated potent antibacterial effects on planktonic bacteria and mono-species biofilms at the concentrations of 50-200 µg/mL against Streptococcus mutans, Streptococcus sobrinus, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Enterococccus faecalis. Moreover, Nano-CHX effectively suppressed multi-species biofilms such as S. mutans, F. nucleatum, A. actinomycetemcomitans and Porphyromonas gingivalis up to 72 h. CONCLUSIONS/SIGNIFICANCE: This pioneering study demonstrates the potent antibacterial effects of the Nano-CHX on oral biofilms, and it may be developed as a novel and promising anti-biofilm agent for clinical use.

  19. A protein-repellent and antibacterial nanocomposite for Class-V restorations to inhibit periodontitis-related pathogens.

    Science.gov (United States)

    Wang, Lin; Xie, Xianju; Imazato, Satoshi; Weir, Michael D; Reynolds, Mark A; Xu, Hockin H K

    2016-10-01

    The objectives of this study were to develop a bioactive dental composite and investigate the effects of 2-methacryloyloxyethyl phosphorylcholine (MPC) and dimethylaminohexadecyl methacrylate (DMAHDM) in Class V composite on mechanical properties, water sorption, protein adsorption, and inhibition of four species of periodontitis-related biofilms for the first time. The resin consisted of ethoxylated bisphenol A dimethacrylate (EBPADMA) and pyromellitic glycerol dimethacrylate (PMGDM). DMAHDM, MPC and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into the resin. Four species (Porphyromonas gingivalis, Prevotella intermedia, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum) were tested for biofilm colony-forming units (CFU), live/dead, metabolic activity, and polysaccharide production. The results showed that adding DMAHDM and MPC to the composite did not compromise the mechanical properties (p>0.1), with acceptable water sorption values. Composite with 3% MPC reduced protein adsorption to 1/9 that of a commercial composite (porders of magnitude via 3% DMAHDM+3% MPC, compared to control. The inhibition efficacy for the four species was: P. gingivalis>P intermedia=A. actinomycetemcomitans>F. nucleatum. In conclusion, a novel bioactive composite with 3% DMAHDM and 3% MPC achieved the greatest reduction in biofilm growth, metabolic activity and polysaccharide of four periodontal pathogens. The new composite is promising for Class V restorations especially with subgingival margins to inhibit periodontal pathogens, combat periodontitis and protect the periodontium. PMID:27287170

  20. The pathogenic persona of community-associated oral streptococci.

    Science.gov (United States)

    Whitmore, Sarah E; Lamont, Richard J

    2011-07-01

    The mitis group streptococci (MGS) are widespread in the oral cavity and are traditionally associated with oral health. However, these organisms have many attributes that contribute to the development of pathogenic oral communities. MGS adhere rapidly to saliva-coated tooth surfaces, thereby providing an attachment substratum for more overtly pathogenic organisms such as Porphyromonas gingivalis, and the two species assemble into heterotypic communities. Close physical association facilitates physiologic support, and pathogens such as Aggregatibacter actinomycetemcomitans display resource partitioning to favour carbon sources generated by streptococcal metabolism. MGS exchange information with community members through a number of interspecies signalling systems including AI-2 and contact dependent mechanisms. Signal transduction systems induced in P. gingivalis are based on protein dephosphorylation mediated by the tyrosine phosphatase Ltp1, and converge on a LuxR-family transcriptional regulator, CdhR. Phenotypic responses in P. gingivalis include regulation of hemin uptake systems and gingipain activity, processes that are intimately linked to the virulence of the organism. Furthermore, communities of S. gordonii with P. gingivalis or with A. actinomycetemcomitans are more pathogenic in animal models than the constituent species alone. We propose that MGS should be considered accessory pathogens, organisms whose pathogenic potential only becomes evident in the context of a heterotypic microbial community.

  1. Periodontopathogen profile of healthy and oral lichen planus patients with gingivitis or periodontitis

    Institute of Scientific and Technical Information of China (English)

    Abdullah Seckin Ertugrul; Ugur Arslan; Recep Dursun; Sema Sezgin Hakki

    2013-01-01

    Oral lichen planus (OLP) is a chronic inflammatory disease that is frequently detected in oral tissues. The aim of our study was to identify the prevalence of the detection of periodontopathogenic microorganisms (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola in OLP patients and to compare with this prevalence of periodontopathogenic microorganisms in healthy non-OLP patients. Our study included 27 (18 chronic periodontitis (OLPP) and 9 gingivitis (OLPG)) patients diagnosed with OLP along with 26 (13 chronic periodontitis (HP) and 13 gingivitis (HG)) healthy non-OLP patients. The multiplex polymerase chain reaction (PCR) with subsequent reverse hybridization method (micro-IDent) was used for identifying periodontopathogenic microorganisms present in subgingival plaque samples. The percentages of detection for A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola in subgingival plaque samples taken from OLP patients (OLPG and OLPP) were 18.5%, 85.1%, 81.4%, 88.8%and 74%, respectively. Meanwhile, in the non-OLP patients (HG and HP), these values were 7.6%, 50%, 46.1%, 73%and 57.7%, respectively. Thus, comparing the non-OLP groups with the OLP groups, the periodontopathogens’ percentages of detection in the OLP groups were higher than those in the non-OLP groups. According to our study results, OLP patients have higher levels of infection with A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia and T. denticola than non-OLP patients. We argue that the high percentages in patients with OLP may help identify the importance of periodontopathogenic microorganisms in the progress of periodontal diseases of OLP.

  2. Association between periodontal disease and plasma levels of cholesterol and triglycerides

    Directory of Open Access Journals (Sweden)

    Adriana Jaramillo

    2013-05-01

    Full Text Available Objective: Untreated periodontal disease seems to cause low grade systemic inflammation and blood lipid alteration leading to increased cardiovascular disease risk. To start testing this hypothesis in Colombian patients, a multicentre study was conducted including the three main state capitals: Bogotá, Medellín and Cali. Methods: In this study 192 (28.4% advanced and 256 (37.8% moderate periodontitis patients were  investigated for socio-demographic variables, city of precedence, periodontal parameters, smoking, red complex periodontopathic bacteria, serum antibodies against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans and blood lipids including total cholesterol, HDL, LDL and triglycerides (TG. Those parameters were compared to 229 (33.8% controls having periodontal health or gingivitis. Results: Advanced periodontitis had worst periodontal indexes, than moderate periodontitis and controls. Interestingly, higher HDL and TG levels were present in periodontitis. BMI <30 and smoking were associated with increased HDL, HDL-35, LDL and TG, while glycemia >100 mg/dL associated with HDL, HDL-35 and TG. Tannerella forsythia showed a significant association with HDL-35 in bivariate analysis and serum IgG1 against P. gingivalis associated with HDL-35 and serum IgG1 against T. forsythia associated with TG and serum IgG2 against A. actinomycetemcomitans correlated with levels of HDL y HDL-35. In logistic regression the periodontitis patients from Cali presented reduced HDL levels as compared to Bogotá and Medellín patients. Presence of IgG1 antibodies against P. gingivalis and A. actinomycetemcomitans correlated with reduced HDL levels. Conclusion: This study confirmed that untreated periodontitis generates alteration in serum lipid levels and systemic bacterial exposure against important periodontopathic bacteria seems to be the biological link. 

  3. Integration of non-oral bacteria into in vitro oral biofilms.

    Science.gov (United States)

    Thurnheer, Thomas; Belibasakis, Georgios N

    2015-01-01

    Biofilms are polymicrobial communities that grow on surfaces in nature. Oral bacteria can spontaneously form biofilms on the surface of teeth, which may compromise the health of the teeth, or their surrounding (periodontal) tissues. While the oral bacteria exhibit high tropism for their specialized ecological niche, it is not clear if bacteria that are not part of the normal oral microbiota can efficiently colonize and grow within oral biofilms. By using an in vitro "supragingival" biofilm model of 6 oral species, this study aimed to investigate if 3 individual bacterial species that are not part of the normal oral microbiota (Eschericia coli, Staphylococcus aureus, Enterococcus faecails) and one not previously tested oral species (Aggregatibacter actinomycetemcomitans) can be incorporated into this established supragingival biofilm model. Staphylococcus aureus and A. actinomycetemcomitans were able to grow efficiently in the biofilm, without disrupting the growth of the remaining species. They localized in sparse small aggregates within the biofilm mass. Enterococcus faecalis and E. coli were both able to populate the biofilm at high numbers, and suppressed the growth of A. oris and S. mutants. Enterococcus faecalis was arranged in a chain-like conformation, whereas E. coli was densely and evenly spread throughout the biofilm mass. In conclusion, it is possible for selected species that are not part of the normal oral microbiota to be introduced into an oral biofilm, under the given experimental micro-environmental conditions. Moreover, the equilibrated incorporation of A. actinomycetemcomitans and S. aureus in this oral biofilm model could be a useful tool in the study of aggressive periodontitis and peri-implantitis, in which these organisms are involved, respectively.

  4. Influencia de los productos de la reacción entre lípidos oxidados (4,5 (E-epoxy-2(E-heptenal y 4,5 (E-epoxy- 2 (E -decenal y lisina sobre la utilización de zinc y calcio: ensayos en células Caco-2.

    Directory of Open Access Journals (Sweden)

    Navarro, María Pilar

    2003-12-01

    Full Text Available The influence of the presence of brown products from the reaction between two oxidized lipids (4,5 (E-epoxy-2(E-heptenal, EH, and 4,5 (E-epoxy-2 (E-decenal, ED and lysine (EH-L and ED-L on zinc and calcium utilization was studied, and compared with a fructosyl-lysine mixture (F-L. Assays were carried out in Caco-2 cells grown in bicameral chambers. The Zn transported across the cell monolayer was significantly lower in the presence of the EH-L, ED-L and F-L samples, specially with EH-L. Significant decreases in Zn uptake were also observed, with no differences between samples. However, calcium transport was no modified. Thus, the assayed lipid-aminoacid brown products seem to have negative effects on Zn availability, whereas Ca availability appears to be unaffected.Se estudió la influencia de la presencia de productos obtenidos en la reacción de dos lípidos oxidados (4,5(E-epoxy-2(E- heptenal, EH, y 4,5(E-epoxy-2(E-decenal, ED con el aminoácido lisina (EH-L y ED-L, sobre la absorción de zinc y calcio, comparándolos frente a una mezcla de fructosil-lisina (F-L. Los ensayos se realizaron con células Caco-2 sembradas en placas bicamerales. La adición de las muestras EH-L, ED-L y F-L al medio de cultivo supuso una reducción significativa en el Zn transportado a través de la monocapa de células, mucho más marcada ante la presencia de EH-L. También se redujo significativamente la captación celular de Zn, sin diferencias entre las distintas muestras ensayadas. Sin embargo, el transporte de Ca no se vio modificado. Por lo tanto, los productos pardos lípido-aminoacídicos ensayados parecen afectar negativamente la disponibilidad del Zn, sin tener efectos notables sobre la del Ca.

  5. Synthesis and Crystal Structure of a Novel Tetranuclear Yttrium Complex With Proline Ligands at High pH Value%高pH下钇-L-脯氨酸四核配合物[Y4(HPrO)4(PRO)2 (μ3-OH)4 (H2O)7] (ClO4)6@6H2O的合成与晶体结构*

    Institute of Scientific and Technical Information of China (English)

    陈德; 张大顺; 李琳; 马宝清; 金天柱

    2000-01-01

    在水溶液中,调节pH至6.3,合成了高氯酸钇与L-脯氨酸的配合物[Y4(HPrO)4(PrO)2(μ3-OH)4(H2O)7](CIO4)6@6H2O单晶,并测定了其晶体结构.该晶体属菱形晶系,R3空间群,晶胞参数:a=b=c=12.786 3(15)A,α=β=γ=100.217(17)0,V=1977.0(4)A3,Z=1,最终偏差因子R1=0.086 9,wR2=0.220 3.每个晶胞以四个Y3+通过四个μ3-OH-离子、六个脯氨酸羧基桥联成四核簇状结构.其中三个Y3+每个均与三个μ3-OH、三个羧基氧原子、两个水分子配位,其配位数为8;另一个Y3+与三个μ3-OH、三个羧基氧原子、一个水分子配位,其配位数为7.

  6. A Modified Glycosaminoglycan, GM-0111, Inhibits Molecular Signaling Involved in Periodontitis.

    Directory of Open Access Journals (Sweden)

    Justin R Savage

    Full Text Available Periodontitis is characterized by microbial infection, inflammation, tissue breakdown, and accelerated loss of alveolar bone matrix. Treatment targeting these multiple stages of the disease provides ways to treat or prevent periodontitis. Certain glycosaminoglycans (GAGs block multiple inflammatory mediators as well as suppress bacterial growth, suggesting that these GAGs may be exploited as a therapeutic for periodontitis.We investigated the effects of a synthetic GAG, GM-0111, on various molecular events associated with periodontitis: growth of Porphyromonas gingivalis (P. gingivalis and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans pathogenic bacteria associated with periodontitis; activation of pro-inflammatory signaling through TLR2 and TLR4 in mouse macrophage RAW 264.7 cells and heterologously expressed HEK 293 cells; osteoclast formation and bone matrix resorption in cultured mouse pre-osteoclasts.(1 GM-0111 suppressed the growth of P. gingivalis and A. actinomycetemcomitans even at 1% (w/v solution. The antibacterial effects of GM-0111 were stronger than hyaluronic acid (HA or xylitol in P. gingivalis at all concentrations and comparable to xylitol in A. actinomycetemcomitans at ≥2% (w/v solution. We also observed that GM-0111 suppressed biofilm formation of P. gingivalis and these effects were much stronger than HA. (2 GM-0111 inhibited TLR-mediated pro-inflammatory cellular signaling both in macrophage and HEK 293 cells with higher selectivity for TLR2 than TLR4 (IC50 of 1-10 ng/mL vs. > 100 μg/mL, respectively. (3 GM-0111 blocked RANKL-induced osteoclast formation (as low as 300 ng/mL and bone matrix resorption. While GM-0111 showed high affinity binding to RANKL, it did not interfere with RANKL/RANK/NF-κB signaling, suggesting that GM-0111 inhibits osteoclast formation by a RANKL-RANK-independent mechanism.We report that GM-0111 inhibits multiple molecular events involved in periodontitis, spanning from the

  7. A Modified Glycosaminoglycan, GM-0111, Inhibits Molecular Signaling Involved in Periodontitis

    Science.gov (United States)

    Savage, Justin R.; Pulsipher, Abigail; Rao, Narayanam V.; Kennedy, Thomas P.; Prestwich, Glenn D.; Ryan, Maria E.; Lee, Won Yong

    2016-01-01

    Background Periodontitis is characterized by microbial infection, inflammation, tissue breakdown, and accelerated loss of alveolar bone matrix. Treatment targeting these multiple stages of the disease provides ways to treat or prevent periodontitis. Certain glycosaminoglycans (GAGs) block multiple inflammatory mediators as well as suppress bacterial growth, suggesting that these GAGs may be exploited as a therapeutic for periodontitis. Methods We investigated the effects of a synthetic GAG, GM-0111, on various molecular events associated with periodontitis: growth of Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) pathogenic bacteria associated with periodontitis; activation of pro-inflammatory signaling through TLR2 and TLR4 in mouse macrophage RAW 264.7 cells and heterologously expressed HEK 293 cells; osteoclast formation and bone matrix resorption in cultured mouse pre-osteoclasts. Results (1) GM-0111 suppressed the growth of P. gingivalis and A. actinomycetemcomitans even at 1% (w/v) solution. The antibacterial effects of GM-0111 were stronger than hyaluronic acid (HA) or xylitol in P. gingivalis at all concentrations and comparable to xylitol in A. actinomycetemcomitans at ≥2% (w/v) solution. We also observed that GM-0111 suppressed biofilm formation of P. gingivalis and these effects were much stronger than HA. (2) GM-0111 inhibited TLR-mediated pro-inflammatory cellular signaling both in macrophage and HEK 293 cells with higher selectivity for TLR2 than TLR4 (IC50 of 1–10 ng/mL vs. > 100 μg/mL, respectively). (3) GM-0111 blocked RANKL-induced osteoclast formation (as low as 300 ng/mL) and bone matrix resorption. While GM-0111 showed high affinity binding to RANKL, it did not interfere with RANKL/RANK/NF-κB signaling, suggesting that GM-0111 inhibits osteoclast formation by a RANKL-RANK-independent mechanism. Conclusions We report that GM-0111 inhibits multiple molecular events involved in

  8. Antimicrobial activity against periodontopathogenic bacteria, antioxidant and cytotoxic effects of various extracts from endemic Thermopsis turcica

    Institute of Scientific and Technical Information of China (English)

    Elif Burcu Bali; Leyla Ak; Glin Akca; Meral Sarper; Mualla Pnar Eli; Ferit Avcu; Mecit Vural

    2014-01-01

    Objective: To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan, Vural &Küçüködük against periodontopathogenic bacteria, its antioxidant activity and cytotoxic effect on various cancer cell lines.Methods: In vitro antimicrobial activities of ethanol, methanol, ethyl acetate (EtAc), n-hexane and water extracts of Thermopsis turcica herb against periodontopathogenic bacteria, Aggregatibacter actinomycetemcomitans ATCC 29523 and Porphyromonas gingivalis ATCC 33277 were tested by agar well diffusion, minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Antioxidant properties of the extracts were evaluated by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity and β-carotene bleaching methods. Amounts of phenolic contents of the extracts were also analysed by using the Folin-Ciocalteu reagent. Additionally, cytotoxic activity of the extracts on androgen-insensitive prostate cancer, androgen-sensitive prostate cancer, chronic myelogenous leukemia and acute promyelocytic leukemia human cancer cell lines were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Human gingival fibroblast cells were used as a control.Results:Our data showed that EtAc extract had the highest antimicrobial effect on Aggregatibacter actinomycetemcomitans (MIC: 1.562 mg/mL, MBC: 3.124 mg/mL) and Porphyromonas gingivalis (MIC: 0.781 mg/mL, MBC: 1.562 mg/mL). In antioxidant assays, EtAc extract exhibited also the highest radical scavenging activity [IC50=(30.0±0.3) μg/mL] and the highest inhibition [(74.35±0.30)%] against lineloic acide oxidation. The amount of phenolic content of it was also the highest [(162.5±1.2) μg/mg gallic acid]. In cytotoxic assay, only ethanol [IC50=(80.00±1.21) μg/mL] and EtAc extract [IC50=(70.0±0.9) μg/mL] were toxic on acute promyelocytic leukemia cells at 20-100 μg/mL (P<0.05). However, no toxic effect was observed on human gingival fibroblast cells

  9. Bacterial inactivation/sterilization by argon plasma treatment on contaminated titanium implant surfaces:In vitro study

    Science.gov (United States)

    Annunziata, Marco; Donnarumma, Giovanna; Caputo, Pina; Nastri, Livia; Guida, Luigi

    2016-01-01

    Background Surface treatment by argon plasma is widely used as the last step of the manufacturing process of titanium implant fixtures before their sterilization by gamma rays. The possibility of using such a technology in the daily clinical practice is particularly fascinating. The aim of the present study was to assess the effects of the argon plasma treatment on different titanium implant surfaces previously exposed In vitro to bacterial contamination. Material and Methods Sterile c.p. titanium implant discs with turned (T, Sa: 0.8 µm ), sandblasted/acid-etched (SAE, Sa: 1.3 µm) and titanium plasma sprayed (TPS, Sa: 3.0µm) surface were used in this study. A strain of Aggregatibacter actinomycetemcomitans ATCC3718 was grown at 37°C under anaerobic conditions for 24 h and then transferred on six discs for each of the three surface types. After 24 hours, a half of the contaminated discs (control group) were directly used to evaluate the colony forming units (CFUs). The other half of the contaminated discs (test group) were treated in an argon plasma chamber for 12 minutes at room temperature prior to be analyzed for CFU counting. All assays were performed using triplicate samples of each material in 3 different experiments. Results When the CFU counting was carried out on control discs, a total of 1.50x106±1.4x105, 1.55x106±7.07x104 and 3.15x106±2.12x105 CFU was respectively assessed for T, SAE and TPS discs, without statistically significant differences among the three surfaces. On the contrary, any trace of bacterial contamination was assessed for titanium discs treated in the argon plasma chamber prior to be analyzed, irrespectively to the implant surface tested. Conclusions Within the limit of this study, reported data suggested that the argon plasma technology could be efficiently used to decontaminate/sterilize previously infected titanium implant surfaces. Key words:Argon plasma, titanium implant surface, Aggregatibacter actinomycetemcomitans. PMID

  10. Synergistic Antibacterial Effects of Nanoparticles Encapsulated with Scutellaria baicalensis and Pure Chlorhexidine on Oral Bacterial Biofilms

    Directory of Open Access Journals (Sweden)

    Ken Cham-Fai Leung

    2016-04-01

    Full Text Available Scutellaria baicalensis (SB is a traditional Chinese medicine for treating infectious and inflammatory diseases. Our recent study shows potent antibacterial effects of nanoparticle-encapsulated chlorhexidine (Nano-CHX. Herein, we explored the synergistic effects of the nanoparticle-encapsulated SB (Nano-SB and Nano-CHX on oral bacterial biofilms. Loading efficiency of Nano-SB was determined by thermogravimetric analysis, and its releasing profile was assessed by high-performance liquid chromatographyusing baicalin (a flavonoid compound of SB as the marker. The mucosal diffusion assay on Nano-SB was undertaken in a porcine model. The antibacterial effects of the mixed nanoparticles (Nano-MIX of Nano-SB and Nano-CHX at 9:1 (w/w ratio were analyzed in both planktonic and biofilm modes of representative oral bacteria. The Nano-MIX was effective on the mono-species biofilms of Streptococcus (S. mutans, S. sobrinus, Fusobacterium (F. nucleatum, and Aggregatibacter (A. actinomycetemcomitans (MIC 50 μg/mL at 24 h, and exhibited an enhanced effect against the multi-species biofilms such as S. mutans, F. nucleatum, A. actinomycetemcomitans, and Porphyromonas (P. gingivalis (MIC 12.5 μg/mL at 24 h that was supported by the findings of both scanning electron microscopy (SEM and confocal scanning laser microscopy (CLSM. This study shows enhanced synergistic antibacterial effects of the Nano-MIX on common oral bacterial biofilms, which could be potentially developed as a novel antimicrobial agent for clinical oral/periodontal care.

  11. Effect of smoking on subgingival microflora of patients with periodontitis in Japan

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    Yamada Satoru

    2011-01-01

    Full Text Available Abstract Background Smoking is a risk factor for periodontitis. To clarify the contribution of smoking to periodontitis, it is essential to assess the relationship between smoking and the subgingival microflora. The aim of this study was to gain an insight into the influence of smoking on the microflora of Japanese patients with periodontitis. Methods Sixty-seven Japanese patients with chronic periodontitis (19 to 83 years old, 23 women and 44 men were enrolled in the present study. They consisted of 30 smokers and 37 non-smokers. Periodontal parameters including probing pocket depth (PPD and bleeding on probing (BOP and oral hygiene status were recorded. Detection of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Fusobacterium nucleatum/periodonticum, Treponema denticola and Campylobacter rectus in subgingival plaque samples was performed by polymerase chain reaction. Association between the detection of periodontopathic bacteria and smoking status was analyzed by multiple logistic regression analysis and chi-square test. Results A statistically significant association was found between having a PPD ≥ 4 mm and detection of T. denticola, P. intermedia, T. forsythia, or C. rectus, with odds ratios ranging from 2.17 to 3.54. A significant association was noted between BOP and the detection of C. rectus or P. intermedia, and smoking, with odds ratios ranging from 1.99 to 5.62. Prevalence of C. rectus was higher in smokers than non-smokers, whereas that of A. actinomycetemcomitans was lower in smokers. Conclusions Within limits, the analysis of the subgingival microbial flora in smokers and non-smokers with chronic periodontitis suggests a relevant association between smoking and colonization by the specific periodontal pathogens including C. rectus.

  12. Modified combustion synthesis of red emitting phosphor Y4Al2O9:Eu3+

    International Nuclear Information System (INIS)

    Yttrium Aluminium Monoclinic (YAM) is Y-richer Phase in Y2O3-Al2O3 system. YAM doped with rare earth ions is a promising material for several technological application including PDP. Conventional solid state reaction techniques require high sintering temperatures (above 1800°C) to prepare phase pure compounds. Though several soft chemical routes have been explored for synthesis of YAG, YAP and YAM, most of these methods are complex. Moreover, phase pure materials are not obtained in one step and prolonged annealing at temperatures around 1000°C is necessary. In this paper, one step combustion synthesis of these compounds is reported using a modified procedure and employing mixed (glycine + urea) fuel. Powder X-ray diffraction study shows phase pure YAM with no detrimental phases corresponding to YAP and YAG. Photoluminescence study reveals intense red emission at 609 nm under 246 nm excitation. (author)

  13. 3-cerro y 4-mundo: los números del banquete en las ofrendas quechuas

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    David Lorente Fernández

    2010-12-01

    Full Text Available Las ofrendas quechuas del sur del Perú son platos alimenticios, banquetes destinados principalmente a la pachamama y los apus, pero también elaborados sistemas matemáticos regidos por operaciones sofisticadas. Sirviéndose de dos números fundamentales, el 3 y el 4, los especialistas rituales son capaces de transmitir mensajes polisémicos. Mediante el número 3 aglutinan clientes, parajes y cerros, en suma, «personas» capaces de interactuar entre sí. El 3 aparece en los k’intus de hojas de coca y en las oraciones que se recitan durante el proceso de realizar la ofrenda. Por el contrario, el número 4 no indica relaciones sino formas espaciales: es un operador geométrico que unifica la servilleta ceremonial (unk’uña, el papel envoltorio y el paquete acabado para representar las cuatro direcciones del mundo y hacer de la ofrenda un mundo en miniatura. Gracias a los dos números el ritualista puede recrear el cosmos, establecer convenios con los dioses y definir nuevas situaciones favorables para la vida de sus clientes.

  14. Corticosterone Regulates the Expression of Neuropeptide Y and Reelin in MLO-Y4 Cells

    OpenAIRE

    Ma, Yuanyuan; Wu, Xiangnan; LI, XIANXIAN; Fu, Jing; Shen, Jiefei; Li, Xiaoyu; Wang, Hang

    2012-01-01

    Osteocytes that have a dendritic appearance are widely believed to form a complex cellular network system and play crucial roles in mechanotransduction as a principal bone mechanosensor, which is the basis of their neuronal-like biology, as previously reported. Neuropeptide Y (NPY) and reelin mRNA, which are brain-specific neurogenic markers, have been identified in osteocytes. However, changes in the production of NPY and reelin in response to specific biochemical stimulation are unknown. In...

  15. Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes

    NARCIS (Netherlands)

    P. Juffer; R.T. Jaspers; P. Lips; A.D. Bakker; J. Klein-Nulend

    2012-01-01

    Lack of physical activity results in muscle atrophy and bone loss, which can be counteracted by mechanical loading. Similar molecular signaling pathways are involved in the adaptation of muscle and bone mass to mechanical loading. Whether anabolic and metabolic factors regulating muscle mass, i.e.,

  16. Periodontitis in cardiovascular disease patients with or without Marfan syndrome--a possible role of Prevotella intermedia.

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    Jun-ichi Suzuki

    Full Text Available BACKGROUND: Although periodontitis is a risk factor for cardiovascular disease (CVD, the influence of periodontitis on Marfan syndrome (MFS with CVD is unclear. The aim of this study was to assess the relationship between periodontal bacterial burden and MSF with CVD. METHODS AND RESULTS: The subjects were patients with MFS with CVD (n = 47; age and gender matched non-MFS CVD patients (n = 48 were employed as controls. Full-mouth clinical measurements, including number of teeth, probing of pocket depth (PD, bleeding on probing (BOP and community periodontal index (CPI were recorded. We also evaluated the existence of three periodontal pathogens, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Prevotella intermedia using polymerase chain reaction assays. Serum antibody titers against the pathogens were also measured. We revealed that MFS with CVD patients had periodontitis more frequently than the age and gender matched non-MFS CVD control subjects. MFS with CVD patients had significantly severer periodontitis, fewer remaining teeth and deeper PD compared to the non-MFS CVD controls. Furthermore, the serum antibody titer level against Prevotella intermedia was significantly lower in MFS plus CVD patients compared to the non-MFS CVD patients. CONCLUSION: Periodontitis may influence the pathophysiology of cardiovascular complications in MFS patients. A specific periodontal pathogen might be a crucial therapeutic target to prevent CVD development.

  17. The Effect of IL-4 Gene Polymorphisms on Cytokine Production in Patients with Chronic Periodontitis and in Healthy Controls

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    Jirina Bartova

    2014-01-01

    Full Text Available Chronic periodontitis (CP is an inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria, and immune mechanisms all play important roles. The aim of this study was to evaluate the occurrence of IL-4 gene polymorphisms in chronic periodontitis and to investigate the association between polymorphisms and cytokines production after bacterial stimulation. Sixty-two subjects (47 CP patients and 15 healthy controls with detected two polymorphisms in the IL-4 gene (-590C/T and intron 3 VNTR were examined. Production of cytokines (IL-1α, IL-1β, IL-4, IL-5, IL-6, IL-10, IL-17, TNFα, INFγ, and VEGF was studied after in vitro stimulation of isolated peripheral blood by mitogens (Pokeweed mitogen, Concanavalin A, dental plaque bacteria (Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, and Prevotella intermedia, and Heat Shock Protein (HSP 70 by the Luminex multiplex cytokine analysis system. The results were correlated with IL-4 genotypes in patients with CP and healthy controls. The mononuclear cells isolated from peripheral blood of CP patients with selected IL-4 polymorphisms significantly altered the production of IFNγ, IL-10, IL-1β, IL-1α, TNFα, and IL-6 after stimulation by HSP 70 or selected bacteria (from P<0.001 to P<0.05. IL-4 gene polymorphisms may influence the function of mononuclear cells to produce not only interleukin-4 but also other cytokines, especially in patients with CP.

  18. Essential Oil from Berries of Lebanese Juniperus excelsa M. Bieb Displays Similar Antibacterial Activity to Chlorhexidine but Higher Cytocompatibility with Human Oral Primary Cells

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    Barbara Azzimonti

    2015-05-01

    Full Text Available Chlorhexidine (CHX, one of the most effective drugs administered for periodontal treatment, presents collateral effects including toxicity when used for prolonged periods; here, we have evaluated the bactericidal potency and the cytocompatibility of Juniperus excelsa M. Bieb essential oil (EO in comparison with 0.05% CHX. The EO was extracted from berries by hydrodistillation and components identified by gas chromatography and mass spectrometry. Bacterial inhibition halo analysis, quantitative cell viability 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl-5-[(phenyl amino carbonyl]-2H-tetrazolium hydroxide assay (XTT, and colony forming unit (CFU count were evaluated against the two biofilm formers Aggregatibacter actinomycetemcomitans and Streptococcus mutans. Finally, cytocompatibility was assessed with human primary gingival fibroblasts (HGF and mucosal keratinocytes (HK. The resulting EO was mainly composed of monoterpene hydrocarbons and oxygenated monoterpenes. An inhibition halo test demonstrated that both bacteria were sensitive to the EO; XTT analysis and CFU counts confirmed that 10-fold-diluted EO determined a statistically significant (p < 0.05 reduction in bacteria count and viability towards both biofilm and planktonic forms in a comparable manner to those obtained with CHX. Moreover, EO displayed higher cytocompatibility than CHX (p < 0.05. In conclusion, EO exhibited bactericidal activity similar to CHX, but a superior cytocompatibility, making it a promising antiseptic alternative to CHX.

  19. Comparative evaluation of subgingival plaque microflora in pregnant and non-pregnant women: A clinical and microbiologic study

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    Rishi Emmatty

    2013-01-01

    Full Text Available Context: Gingival changes in pregnancy have been attributed to changes in the subgingival biofilm related to hormonal variations. Aims: To evaluate the subgingival plaque microflora in pregnant and nonpregnant women to determine if pregnancy induces any alterations in the subgingival plaque and to associate these changes with changes in periodontal status. Settings and Design: Thirty pregnant and 10 nonpregnant women within the age group of 20-35 years having a probing pocket depth (PPD of 3-4 mm were included in the study. The pregnant women were equally divided into 3 groups of 10, each belonging to I, II, and III trimester. Materials and Methods: Plaque index, gingival index, PPD, and microbiologic evaluation for specific bacterial counts for Prevotella intermedia, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Parvimonas micra, and Fusobacterium nucleatum were carried out for all subjects. Statistical Analysis: Mann-Whitney U test. Results: Increase in gingival inflammation was observed in II and III trimester as compared with I trimester and control. Plaque scores did not show any significant difference between pregnant and nonpregnant women. Specific bacterial examination revealed an increase in proportion of P. intermedia in pregnant women of both II and III trimester as compared with I trimester and nonpregnant women. Conclusions: A definite increase in proportions of P. intermedia occurs in subgingival plaque microflora in pregnancy that may be responsible for the exaggerated gingival response.

  20. Antibiofilm activity of Actinobacillus pleuropneumoniae serotype 5 capsular polysaccharide.

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    Michael T Karwacki

    Full Text Available Cell-free extracts isolated from colony biofilms of Actinobacillus pleuropneumoniae serotype 5 were found to inhibit biofilm formation by Staphylococcus aureus, S. epidermidis and Aggregatibacter actinomycetemcomitans, but not by A. pleuropneumoniae serotype 5 itself, in a 96-well microtiter plate assay. Physical and chemical analyses indicated that the antibiofilm activity in the extract was due to high-molecular-weight polysaccharide. Extracts isolated from a mutant strain deficient in the production of serotype 5 capsular polysaccharide did not exhibit antibiofilm activity. A plasmid harboring the serotype 5 capsule genes restored the antibiofilm activity in the mutant extract. Purified serotype 5 capsular polysaccharide also exhibited antibiofilm activity against S. aureus. A. pleuropneumoniae wild-type extracts did not inhibit S. aureus growth, but did inhibit S. aureus intercellular adhesion and binding of S. aureus cells to stainless steel surfaces. Furthermore, polystyrene surfaces coated with A. pleuropneumoniae wild-type extracts, but not with capsule-mutant extracts, resisted S. aureus biofilm formation. Our findings suggest that the A. pleuropneumoniae serotype 5 capsule inhibits cell-to-cell and cell-to-surface interactions of other bacteria. A. pleuropneumoniae serotype 5 capsular polysaccharide is one of a growing number of bacterial polysaccharides that exhibit broad-spectrum, nonbiocidal antibiofilm activity. Future studies on these antibiofilm polysaccharides may uncover novel functions for bacterial polysaccharides in nature, and may lead to the development of new classes of antibiofilm agents for industrial and clinical applications.

  1. Laser Supported Reduction of Specific Microorganisms in the Periodontal Pocket with the Aid of an Er,Cr:YSGG Laser: A Pilot Study

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    N. Gutknecht

    2015-01-01

    Full Text Available Objective. The aim of this study was to evaluate the effectiveness of a radial firing tip of an Er,Cr:YSGG laser as an adjunct to a nonsurgical periodontal treatment. Methods. Twelve patients with chronic or aggressive periodontitis were treated by conventional periodontal treatment using ultrasonic devices and hand instruments and, additionally, in two quadrants with an Er,Cr:YSGG laser. A new radial firing tip (RFPT 14-5, Biolase was used with 1.5 W, 30 Hz, 11% air, 20% water, and pulse duration 140 μs. Microbiological smears were taken before treatment, one day after lasing, and three and six months after lasing. Pocket depths of all periodontal sites were measured before and six months after treatment. Results. The total bacterial load of Prevotella intermedia, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans inside the pocket was reduced significantly throughout the whole examination time. Greater pocket depth reductions were observed in all groups. There was a slight higher reduction of pocket depth in the lased group after six months. Conclusions. These results support the thesis that Er,Cr:YSGG laser supported periodontal treatment leads to a significant reduction of periopathogenes and thereby helps the maintenance of periodontal health.

  2. Evaluation of the efficacy of a new oral gel containing carvacrol and thymol for home oral care in the management of chronic periodontitis using PCR analysis: a microbiological pilot study.

    Science.gov (United States)

    Lauritano, D; Pazzi, D; Iapichino, A; Gaudio, R M; Di Muzio, M; Lo Russo, L; Pezzetti, F

    2016-01-01

    The use of chemical devices for domestic oral hygiene in periodontal patients has led to new treatment strategies aiming primarily at a control of infection. Over the last few years, carvacrol and thymol (CT) have been subjected to many scientific and medical studies. The purpose of the present study was to assess the effect of CT on the red complex bacteria using Polymerase Chain Reaction (PCR) for microbiological analysis. Five patients with a diagnosis of chronic periodontitis in the age group >25 years, were selected. None of these patients had received any surgical or non-surgical periodontal therapy and demonstrated radiographic evidence of moderate bone loss. After scaling and root planning, patients received a CT gel to be used at home. Four non-adjacent sites in separate quadrants were selected in each patient for monitoring, based on criteria that the sites localize chronic periodontitis. Microbial analysis (MA) was analyzed at baseline and at day 15. SPSS program was used for statistical purposes and a paired samples correlation was performed at the end of the observation period. Although an absolute reduction was observed among the studied bacteria (i.e. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Campylobacter rectus and Total bacteria loading) none reach a statistical significant value. The present study demonstrated that CT gel has a small impact on oral biofilm. Additional studies are needed to detect the efficacy of CT gel. PMID:27469559

  3. Bacterial load of periodontal pathogens among italian patients with chronic periodontitis: a comparative study of three different areas.

    Science.gov (United States)

    Lauritano, D; Martinelli, M; Mucchi, D; Palmieri, A; Lo Muzio, L; Carinci, F

    2016-01-01

    The aim of the present study was to evaluate the mean bacterial load of some periodontal pathogenic bacteria in Italian patients affected by chronic periodontitis. The sample consisted of 1,762 patients with a clinical diagnosis of chronic periodontitis based on the criteria of the American Academy of Periodontology sampled in the period 2013-2015; 1,323 patients were from Northern Italy, 317 from Central Italy and 122 from Southern Italy. Samples for microbiological analysis were collected from the four sites of the greatest probing depth in each patient and then processed by quantitative polymerase chain reaction. Periodontal pathogens have the following percentage respect to total bacteria load: Aggregatibacter actinomycetemcomitans 0.1%, Campylobacter rectus 2%, Fusobacterium nucleatum 8%, Porphyromonas gingivalis 6%, Treponema denticola 2% and Tannerella forsythia 1.5%. There are significant differences in bacterial load among the different geographical areas both for the total bacterial and for the single species. The results of our study in this Italian population showed that a different geographic distribution exists among periodontal pathogens. We hypothesize that these differences in bacterial load could be related to genetic and environmental factors. Additional studies are necessary to confirm these data and to get more insight on additional factors, which may play a role in periodontal pathogens in different geographic areas. PMID:27469562

  4. Effect of metalloporphyrins on red autofluorescence from oral bacteria.

    Science.gov (United States)

    Volgenant, Catherine M C; van der Veen, Monique H; de Soet, Johannes J; ten Cate, Jacob M

    2013-06-01

    The aim of this study was to assess the red autofluorescence from bacterial species related to dental caries and periodontitis in the presence of different nutrients in the growth medium. Bacteria were grown anaerobically on tryptic soy agar (TSA) supplemented with nutrients, including magnesium-porphyrins from spinach and iron-porphyrins from heme. The autofluorescence was then assessed at 405 nm excitation. On the TSA without additives, no autofluorescence was observed from any of the species tested. On the TSA containing sheep blood, red autofluorescence was observed only from Parvimonas micra. When the TSA was supplemented with blood, hemin, and vitamin K, red autofluorescence was observed from Actinomyces naeslundii, Bifidobacterium dentium, and Streptococcus mutans. Finally, on the TSA supplemented with spinach extract, red autofluorescence was observed from Aggregatibacter actinomycetemcomitans, A. naeslundii, Enterococcus faecalis, Fusobacterium nucleatum, Lactobacillus salivarius, S. mutans, and Veillonella parvula. We conclude that the bacteria related to dental caries and periodontal disease exhibit red autofluorescence. The autofluorescence characteristics of the tested strains depended on the nutrients present, such as metalloporphyrins, suggesting that the metabolic products of the oral biofilm could be responsible for red autofluorescence. PMID:23659237

  5. Improving photodynamic inactivation of bacteria in dentistry: highly effective and fast killing of oral key pathogens with novel tooth-colored type-II photosensitizers.

    Science.gov (United States)

    Späth, Andreas; Leibl, Christoph; Cieplik, Fabian; Lehner, Karin; Regensburger, Johannes; Hiller, Karl-Anton; Bäumler, Wolfgang; Schmalz, Gottfried; Maisch, Tim

    2014-06-26

    Increasing antibiotic resistances in microorganisms create serious problems in public health. This demands alternative approaches for killing pathogens to supplement standard treatment methods. Photodynamic inactivation of bacteria (PIB) uses light activated photosensitizers (PS) to generate reactive oxygen species immediately upon illumination, inducing lethal phototoxicity. Positively charged phenalen-1-one derivatives are a new generation of PS for light-mediated killing of pathogens with outstanding singlet oxygen quantum yield ΦΔ of >97%. Upon irradiation with a standard photopolymerizer light (bluephase C8, 1260 ± 50 mW/cm(2)) the PS showed high activity against the oral key pathogens Enterococcus faecalis, Actinomyces naeslundii, Streptococcus mutans, and Aggregatibacter actinomycetemcomitans. At a concentration of 10 μM, a maximum efficacy of more than 6 log10 steps (≥ 99.9999%) of bacteria killing is reached in less than 1 min (light dose 50 J/cm(2)) after one single treatment. The pyridinium substituent as positively charged moiety is especially advantageous for antimicrobial action. PMID:24884918

  6. Inflammatory and Bone Remodeling Responses to the Cytolethal Distending Toxins

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    Georgios N. Belibasakis

    2014-04-01

    Full Text Available The cytolethal distending toxins (CDTs are a family of exotoxins produced by a wide range of Gram-negative bacteria. They are known for causing genotoxic stress to the cell, resulting in growth arrest and eventually apoptotic cell death. Nevertheless, there is evidence that CDTs can also perturb the innate immune responses, by regulating inflammatory cytokine production and molecular mediators of bone remodeling in various cell types. These cellular and molecular events may in turn have an effect in enhancing local inflammation in diseases where CDT-producing bacteria are involved, such as Aggregatibacter actinomycetemcomitans, Haemophilus ducreyi, Campylobacter jejuni and Helicobacter hepaticus. One special example is the induction of pathological bone destruction in periodontitis. The opportunistic oral pathogen Aggregatibatcer actinoycemetemcomitans, which is involved in the aggressive form of the disease, can regulate the molecular mechanisms of bone remodeling in a manner that favors bone resorption, with the potential involvement of its CDT. The present review provides an overview of all known to-date inflammatory or bone remodeling responses of CDTs produced by various bacterial species, and discusses their potential contribution to the pathogenesis of the associated diseases.

  7. Comparison of the detection of periodontal pathogens in bacteraemia after tooth brushing by culture and molecular techniques

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    Figuero, Elena; González, Itziar; O´Connor, Ana; Diz, Pedro; Álvarez, Maximiliano; Herrera, David; Sanz, Mariano

    2016-01-01

    Background The prevalence and amounts of periodontal pathogens detected in bacteraemia samples after tooth brushing-induced by means of four diagnostic technique, three based on culture and one in a molecular-based technique, have been compared in this study. Material and Methods Blood samples were collected from thirty-six subjects with different periodontal status (17 were healthy, 10 with gingivitis and 9 with periodontitis) at baseline and 2 minutes after tooth brushing. Each sample was analyzed by three culture-based methods [direct anaerobic culturing (DAC), hemo-culture (BACTEC), and lysis-centrifugation (LC)] and one molecular-based technique [quantitative polymerase chain reaction (qPCR)]. With culture any bacterial isolate was detected and quantified, while with qPCR only Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were detected and quantified. Descriptive analyses, ANOVA and Chi-squared tests, were performed. Results Neither BACTEC nor qPCR detected any type of bacteria in the blood samples. Only LC (2.7%) and DAC (8.3%) detected bacteraemia, although not in the same patients. Fusobacterium nucleatum was the most frequently detected bacterial species. Conclusions The disparity in the results when the same samples were analyzed with four different microbiological detection methods highlights the need for a proper validation of the methodology to detect periodontal pathogens in bacteraemia samples, mainly when the presence of periodontal pathogens in blood samples after tooth brushing was very seldom. Key words:Bacteraemia, periodontitis, culture, PCR, tooth brushing. PMID:26946197

  8. In Vitro Antimicrobial and Antiproliferative Activity of Amphipterygium adstringens

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    A. Rodriguez-Garcia

    2015-01-01

    Full Text Available Amphipterygium adstringens is a plant widely used in Mexican traditional medicine for its known anti-inflammatory and antiulcer properties. In this work, we evaluated the in vitro antimicrobial and antiproliferative activities of the methanolic extract of A. adstringens against oral pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Candida albicans, and Candida dubliniensis, using microdilution (MIC and agar diffusion methods (MBC, and the antiproliferative activity evaluating total growth inhibition (TGI by staining the protein content with sulforhodamine B (SRB, using nine human cancer cell lines. Crude extract (CE of A. adstringens showed some degree of activity against one or more of the strains with a MIC from 0.125 mg/mL to 63 mg/mL and MBC from 1.6 to 6.3 mg/mL and cytotoxic activity, particularly against NCI-ADR/RES, an ovarian cell line expressing multiple resistance drugs phenotype. The CE is a complex mixture of possible multitarget metabolites that could be responsible for both antimicrobial and antiproliferative activities, and further investigation is required to elucidate the identity of active compounds. Nevertheless the CE itself is useful in the development of new antimicrobial treatment based on natural products to prevent oral diseases and as alternative natural source for cancer treatment and prevention.

  9. Detection of five potentially periodontal pathogenic bacteria in peri-implant disease: A comparison of PCR and real-time PCR.

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    Schmalz, Gerhard; Tsigaras, Sandra; Rinke, Sven; Kottmann, Tanja; Haak, Rainer; Ziebolz, Dirk

    2016-07-01

    The aim of this study was to compare the microbial analysis methods of polymerase chain reaction (PCR) and real-time PCR (RT-PCR) in terms of detection of five selected potentially periodontal pathogenic bacteria in peri-implant disease. Therefore 45 samples of healthy, mucositis and peri-implantitis (n = 15 each) were assessed according to presence of the following bacteria using PCR (DNA-strip technology) and RT-PCR (fluorescent dye SYBR green-system): Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Treponema denticola (Td), Tanerella forsythia (Tf), and Fusobacterium nucleatum (Fn). There were no significant correlations between the bacterial and disease patterns, so the benefit of using microbiological tests for the diagnosis of peri-implant diseases is questionable. Correlations between the methods were highest for Tf (Kendall's Tau: 0.65, Spearman: 0.78), Fn (0.49, 0.61) and Td (0.49, 0.59). For Aa (0.38, 0.42) and Pg (0.04, 0.04), lower correlation values were detected. Accordingly, conventional semi-quantitative PCR seems to be sufficient for analyzing potentially periodontal pathogenic bacterial species.

  10. Longitudinal study on clinical and microbial analysis of periodontal status in pregnancy.

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    Machado, Fernanda Campos; Cesar, Dionéia Evangelista; Apolônio, Ana Carolina Morais; Ribeiro, Luiz Claudio; Ribeiro, Rosangela Almeida

    2016-01-01

    This study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (FISH) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks postpartum. Of 31 women evaluated during pregnancy, 24 returned for the 48-h and 18 for their 8-week exams postpartum. Probing depth (PD), bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected, and FISH was used to identify the numbers of eight periodontal pathogens. Friedman test was used to compare differences between follow-up examinations, followed by a multiple comparison test for a post hoc pairwise comparison. Clinically, a significantly greater number of teeth with PD = 4-5 mm were found during pregnancy than on postpartum examinations. Microbial analysis showed a statistically significant decrease in cell count over the study period for Prevotella nigrescens. P. intermedia, Campylobacter rectus, and Porphyromonas gingivalis also decrease, although not significantly, and Aggregatibacter actinomycetemcomitans increased. No significant changes were found for Fusobacterium nucleatum, Treponema denticola, or Tannerella forsythia. Our data demonstrate a change in the subgingival microbiota during pregnancy, at least for P. nigrescens. PMID:27556678

  11. Variation of perimplant biofilm induced by non surgical periodontal therapy and the use of probiotics

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    Marcello Gatti

    2010-12-01

    Full Text Available Thanks to improved surgical tecniques the use of dental implants has increased greatly. However, high rates of osseointegrated correctly implants, over the years are undermined by disease of bacterial etiology in the perimplant zone, especially by Gram negative anaerobes such as in gingivitis and periodontitis, in particular: Fusobacterium spp.(F., Treponema denticola (T.d., Tannerella forsythensis (T.f., Aggregatibacter actinomycetemcomitans (A.a., Prevotella intermedia (P.i. e Porphyromonas gingivalis (P.g.. The mechanic treatment (MS results in a reduction of the total bacterial count (TBC and a slight change in the subgingival bacterial microflora towards the less pathogenic species and more like those of a healthy periodontium.Also the use of a probiotic in the form of buccal tablets of Lactobacillus reuteri (L.r., as demonstrated in this study, is thought to improve and modulate the composition of plaque, as it is able to exert an inhibitory effect on oral bacteria that support caries, gingivitis, periodontal and perimplant disease with a combination of different mechanisms.

  12. Periodontal disease in individuals with Down Syndrome: genetic focus

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    Lícia Bezerra Cavalcante

    2009-12-01

    Full Text Available Fundamental concepts of etiology, inheritance and clinical characteristics of Down syndrome are used in this review as a basis for submission of studies that focus on periodontal disease in individuals with Down syndrome, since almost 100% of them develop the disease in adult life. It is believed that in association with environmental and cultural factors related to hygiene and disabilities of coordination, the immunological characteristics that are found altered in individuals with Down syndrome, such as deficient neutrophil chemotaxis and reduced number of mature T lymphocytes, may contribute to the greater prevalence and severity of periodontal involvement in patients with Down syndrome. Moreover, the pattern of periodontal destruction observed in individuals with Down syndrome is consistent with aggressive periodontitis, with a predominance of periodontopathogens such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythensis during childhood and adolescence of Down’s syndrome patients. It is possible to note a relationship between the development of molecular techniques and the evolution of knowledge about Down syndrome, for example: identification of the trisomy syndrome by observing only part of chromosome 21 (distal long arm; identification of genes in this trisomic region and the pattern of superexpression (or not of these genes. Moreover, in this review future perspectives are presented with regard to better understanding Down syndrome in the genetic context, which will reflect in more individualized and effective clinical treatments that will provide these patients with a better quality of life.

  13. Evaluation of the efficacy of a probiotic drink containing Lactobacillus casei on the levels of periodontopathic bacteria in periodontitis: A clinico-microbiologic study

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    Faizuddin Imran

    2015-01-01

    Full Text Available Background and Objectives: This study was designed to evaluate whether the oral administration of lactobacilli could change the bacterial population in subgingival plaque. Subjects and Methods: Forty-two healthy volunteers with chronic generalized mild to moderate periodontitis were given a probiotic drink containing Lactobacillus casei for 1 month. Subgingival plaque samples were collected at baseline, after which the patients were asked to consume the probiotic drink once daily for 1 month. At the 1 month interval, plaque samples were collected, and the drink discontinued. The patients were recalled at 2 months interval for collection of the final samples. The bacterial amounts in the plaque samples were analyzed by multiplex polymerase chain reaction procedure. Results: Of the three periodontopathic bacteria selected, Porphyromonas gingivalis showed highly significant reductions in the bacterial levels at 1-month and 2 months intervals. In comparison, Aggregatibacter actinomycetemcomitans, when present higher than 10 × 10 3 at baseline, and Prevotella intermedia present higher than 2 × 10 3 at baseline, showed moderately significant reduction in their numbers. Interpretation and Conclusion: Oral administration of the probiotic lactobacilli reduced the numerical sum of the three selected periodontopathic bacteria and could contribute to the beneficial effects on periodontal conditions.

  14. A natural therapeutic approach for the treatment of periodontitis by MK615.

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    Morimoto-Yamashita, Yoko; Kawakami, Yoshiko; Tatsuyama, Syoko; Miyashita, Keiko; Emoto, Makiko; Kikuchi, Kiyoshi; Kawahara, Ko-ichi; Tokuda, Masayuki

    2015-11-01

    Periodontitis is a chronic inflammatory disease that affects the tooth-supporting tissues. Gingival fibroblasts are the most abundant cells in periodontal tissues and they participate actively in the host inflammatory response to periodontal pathogens that is known to mediate local tissue destruction in periodontitis. The Japanese apricot, known as Ume in Japanese, has been a traditional Japanese medicine for centuries and is a familiar and commonly consumed food. The health benefits of Ume are widely recognized and have been confirmed in recent studies showing that MK615, an extract of compounds from Ume, has strong anticancer and anti-inflammatory effects. However, the potential role of MK615 in oral health is unknown. We hypothesized that the anti-inflammatory activities of MK615 could be exploited to inhibit the effects of lipopolysaccharide (LPS) produced by periodontal bacterial pathogens, such as Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Here, we show that LPS-induced interleukin (IL)-6 and IL-8 production by gingival fibroblasts was dose-dependently inhibited by MK615. As a potent inhibitor of the inflammatory responses induced by periodontal pathogens, MK615 merits further testing as a therapeutic agent in inflammatory diseases such as periodontitis. PMID:26305447

  15. Staphylococcus epidermidis Biofilms: Functional Molecules, Relation to Virulence, and Vaccine Potential

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    Mack, Dietrich; Davies, Angharad P.; Harris, Llinos G.; Knobloch, Johannes K. M.; Rohde, Holger

    Medical device-associated infections, most frequently caused by Staphylococcus epidermidis and Staphylococcus aureus, are of increasing importance in modern medicine. The formation of adherent, multilayered bacterial biofilms is crucial in the pathogenesis of these infections. Polysaccharide intercellular adhesin (PIA), a homoglycan of β-1,6-linked 2-acetamido-2-deoxy-d-glucopyranosyl residues, of which about 15% are non-N-acetylated, is central to biofilm accumulation in staphylococci. It transpires that polysaccharides - structurally very similar to PIA - are also key to biofilm formation in a number of other organisms including the important human pathogens Escherichia coli, Aggregatibacter (Actinobacillus) actinomycetemcomitans, Yersinia pestis, and Bordetella spp. Apparently, synthesis of PIA and related polysaccharides is a general feature important for biofilm formation in diverse bacterial genera. Current knowledge about the structure and biosynthesis of PIA and related polysaccharides is reviewed. Additionally, information on their role in pathogenesis of biomaterial-related and other type of infections and the potential use of PIA and related compounds for prevention of infection is evaluated.

  16. Identification of periodontopathogen microorganisms by PCR technique

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    Milićević Radovan

    2008-01-01

    Full Text Available INTRODUCTION Periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. The onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. Gram negative bacteria, mainly strict anaerobes, play the major role. OBJECTIVE The present study aimed to assess the presence of the main types of microorganisms involved in the aetiopathogenesis of periodontal disease: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia and Prevotella intermedia in different samples collected from the oral cavity of 90 patients diagnosed with periodontitis. METHOD Bacterial DNA detection was performed in diverse biological materials, namely in dental plaque, gingival tissue and saliva, by means of multiplex PCR, a technique that allows simultaneous identification of two different bacterial genomes. RESULTS In the dental plaque of the periodontitis patients, Treponema denticola dominated. In the gingival tissue, Tannerella forsythia and Treponema denticola were the microbiota most frequently detected, whilst in saliva Treponema denticola and Eikenella corrodens were found with the highest percentage. CONCLUSION The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria.

  17. Differentiation of oral bacteria in in vitro cultures and human saliva by secondary electrospray ionization - mass spectrometry

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    Bregy, Lukas; Müggler, Annick R.; Martinez-Lozano Sinues, Pablo; García-Gómez, Diego; Suter, Yannick; Belibasakis, Georgios N.; Kohler, Malcolm; Schmidlin, Patrick R.; Zenobi, Renato

    2015-10-01

    The detection of bacterial-specific volatile metabolites may be a valuable tool to predict infection. Here we applied a real-time mass spectrometric technique to investigate differences in volatile metabolic profiles of oral bacteria that cause periodontitis. We coupled a secondary electrospray ionization (SESI) source to a commercial high-resolution mass spectrometer to interrogate the headspace from bacterial cultures and human saliva. We identified 120 potential markers characteristic for periodontal pathogens Aggregatibacter actinomycetemcomitans (n = 13), Porphyromonas gingivalis (n = 70), Tanerella forsythia (n = 30) and Treponema denticola (n = 7) in in vitro cultures. In a second proof-of-principle phase, we found 18 (P. gingivalis, T. forsythia and T. denticola) of the 120 in vitro compounds in the saliva from a periodontitis patient with confirmed infection with P. gingivalis, T. forsythia and T. denticola with enhanced ion intensity compared to two healthy controls. In conclusion, this method has the ability to identify individual metabolites of microbial pathogens in a complex medium such as saliva.

  18. Microarray analysis of microbiota of gingival lesions in noma patients.

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    Huyghe, Antoine; François, Patrice; Mombelli, Andrea; Tangomo, Manuela; Girard, Myriam; Baratti-Mayer, Denise; Bolivar, Ignacio; Pittet, Didier; Schrenzel, Jacques

    2013-01-01

    Noma (cancrum oris) is a gangrenous disease of unknown etiology affecting the maxillo-facial region of young children in extremely limited resource countries. In an attempt to better understand the microbiological events occurring during this disease, we used phylogenetic and low-density microarrays targeting the 16S rRNA gene to characterize the gingival flora of acute noma and acute necrotizing gingivitis (ANG) lesions, and compared them to healthy control subjects of the same geographical and social background. Our observations raise doubts about Fusobacterium necrophorum, a previously suspected causative agent of noma, as this species was not associated with noma lesions. Various oral pathogens were more abundant in noma lesions, notably Atopobium spp., Prevotella intermedia, Peptostreptococcus spp., Streptococcus pyogenes and Streptococcus anginosus. On the other hand, pathogens associated with periodontal diseases such as Aggregatibacter actinomycetemcomitans, Capnocytophaga spp., Porphyromonas spp. and Fusobacteriales were more abundant in healthy controls. Importantly, the overall loss of bacterial diversity observed in noma samples as well as its homology to that of ANG microbiota supports the hypothesis that ANG might be the immediate step preceding noma.

  19. Microarray analysis of microbiota of gingival lesions in noma patients.

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    Antoine Huyghe

    Full Text Available Noma (cancrum oris is a gangrenous disease of unknown etiology affecting the maxillo-facial region of young children in extremely limited resource countries. In an attempt to better understand the microbiological events occurring during this disease, we used phylogenetic and low-density microarrays targeting the 16S rRNA gene to characterize the gingival flora of acute noma and acute necrotizing gingivitis (ANG lesions, and compared them to healthy control subjects of the same geographical and social background. Our observations raise doubts about Fusobacterium necrophorum, a previously suspected causative agent of noma, as this species was not associated with noma lesions. Various oral pathogens were more abundant in noma lesions, notably Atopobium spp., Prevotella intermedia, Peptostreptococcus spp., Streptococcus pyogenes and Streptococcus anginosus. On the other hand, pathogens associated with periodontal diseases such as Aggregatibacter actinomycetemcomitans, Capnocytophaga spp., Porphyromonas spp. and Fusobacteriales were more abundant in healthy controls. Importantly, the overall loss of bacterial diversity observed in noma samples as well as its homology to that of ANG microbiota supports the hypothesis that ANG might be the immediate step preceding noma.

  20. Oxidative Stress Parameters in Saliva and Its Association with Periodontal Disease and Types of Bacteria

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    Jose Manuel Almerich-Silla

    2015-01-01

    Full Text Available Objective. To determine the association between oxidative stress parameters with periodontal disease, bleeding, and the presence of different periodontal bacteria. Methods. A cross-sectional study in a sample of eighty-six patients, divided into three groups depending on their periodontal status. Thirty-three with chronic periodontitis, sixteen with gingivitis, and thirty-seven with periodontal healthy as control. Oxidative stress biomarkers (8-OHdG and MDA, total antioxidant capacity (TAOC, and the activity of two antioxidant enzymes (GPx and SOD were determined in saliva. Subgingival plaque samples were obtained from the deepest periodontal pocket and PCR was used to determine the presence of the 6 fimA genotypes of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, and Treponema denticola. Results. Periodontal disease was found to be associated with increased oxidative stress parameter levels. These levels rose according to the number and type of different periodontal bacteria found in the periodontal pockets. The presence of different types of periodontal bacteria is predictive independent variables in linear regresion models of oxidative stress parameters as dependent variable, above all 8-OHdG. Conclusions. Oxidative stress parameter levels are correlated with the presence of different types of bacteria. Determination of these levels and periodontal bacteria could be a potent tool for controlling periodontal disease development.

  1. Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults.

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    Carrouel, Florence; Viennot, Stéphane; Santamaria, Julie; Veber, Philippe; Bourgeois, Denis

    2016-01-01

    In oral health, the interdental spaces are a real ecological niche for which the body has few or no alternative defenses and where the traditional daily methods for control by disrupting biofilm are not adequate. The interdental spaces are the source of many hypotheses regarding their potential associations with and/or causes of cardiovascular disease, diabetes, chronic kidney disease, degenerative disease, and depression. This PCR study is the first to describe the interdental microbiota in healthy adults aged 18-35 years-old with reference to the Socransky complexes. The complexes tended to reflect microbial succession events in developing dental biofilms. Early colonizers included members of the yellow, green, and purple complexes. The orange complex bacteria generally appear after the early colonizers and include many putative periodontal pathogens, such as Fusobacterium nucleatum. The red complex (Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) was considered the climax community and is on the list of putative periodontal pathogens. The 19 major periodontal pathogens tested were expressed at various levels. F. nucleatum was the most abundant species, and the least abundant were Actinomyces viscosus, P. gingivalis, and Aggregatibacter actinomycetemcomitans. The genome counts for Eikenella corrodens, Campylobacter concisus, Campylobacter rectus, T. denticola, and Tannerella forsythensis increased significantly with subject age. The study highlights the observation that bacteria from the yellow complex (Streptococcus spp., S. mitis), the green complex (E. corrodens, Campylobacter gracilis, Capnocytophaga ochracea, Capnocytophaga sputigena, A. actinomycetemcomitans), the purple complex (Veillonella parvula, Actinomyces odontolyticus) and the blue complex (A. viscosus) are correlated. Concerning the orange complex, F. nucleatum is the most abundant species in interdental biofilm. The red complex, which is recognized as the most important

  2. Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults

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    Carrouel, Florence; Viennot, Stéphane; Santamaria, Julie; Veber, Philippe; Bourgeois, Denis

    2016-01-01

    In oral health, the interdental spaces are a real ecological niche for which the body has few or no alternative defenses and where the traditional daily methods for control by disrupting biofilm are not adequate. The interdental spaces are the source of many hypotheses regarding their potential associations with and/or causes of cardiovascular disease, diabetes, chronic kidney disease, degenerative disease, and depression. This PCR study is the first to describe the interdental microbiota in healthy adults aged 18–35 years-old with reference to the Socransky complexes. The complexes tended to reflect microbial succession events in developing dental biofilms. Early colonizers included members of the yellow, green, and purple complexes. The orange complex bacteria generally appear after the early colonizers and include many putative periodontal pathogens, such as Fusobacterium nucleatum. The red complex (Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola) was considered the climax community and is on the list of putative periodontal pathogens. The 19 major periodontal pathogens tested were expressed at various levels. F. nucleatum was the most abundant species, and the least abundant were Actinomyces viscosus, P. gingivalis, and Aggregatibacter actinomycetemcomitans. The genome counts for Eikenella corrodens, Campylobacter concisus, Campylobacter rectus, T. denticola, and Tannerella forsythensis increased significantly with subject age. The study highlights the observation that bacteria from the yellow complex (Streptococcus spp., S. mitis), the green complex (E. corrodens, Campylobacter gracilis, Capnocytophaga ochracea, Capnocytophaga sputigena, A. actinomycetemcomitans), the purple complex (Veillonella parvula, Actinomyces odontolyticus) and the blue complex (A. viscosus) are correlated. Concerning the orange complex, F. nucleatum is the most abundant species in interdental biofilm. The red complex, which is recognized as the most important

  3. Quantitative PCR analysis of salivary pathogen burden in periodontitis.

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    Salminen, Aino; Kopra, K A Elisa; Hyvärinen, Kati; Paju, Susanna; Mäntylä, Päivi; Buhlin, Kåre; Nieminen, Markku S; Sinisalo, Juha; Pussinen, Pirkko J

    2015-01-01

    Our aim was to investigate the value of salivary concentrations of four major periodontal pathogens and their combination in diagnostics of periodontitis. The Parogene study included 462 dentate subjects (mean age 62.9 ± 9.2 years) with coronary artery disease (CAD) diagnosis who underwent an extensive clinical and radiographic oral examination. Salivary levels of four major periodontal bacteria were measured by quantitative real-time PCR (qPCR). Median salivary concentrations of Porphyromonas gingivalis, Tannerella forsythia, and Prevotella intermedia, as well as the sum of the concentrations of the four bacteria, were higher in subjects with moderate to severe periodontitis compared to subjects with no to mild periodontitis. Median salivary Aggregatibacter actinomycetemcomitans concentrations did not differ significantly between the subjects with no to mild periodontitis and subjects with moderate to severe periodontitis. In logistic regression analysis adjusted for age, gender, diabetes, and the number of teeth and implants, high salivary concentrations of P. gingivalis, T. forsythia, and P. intermedia were significantly associated with moderate to severe periodontitis. When looking at different clinical and radiographic parameters of periodontitis, high concentrations of P. gingivalis and T. forsythia were significantly associated with the number of 4-5 mm periodontal pockets, ≥6 mm pockets, and alveolar bone loss (ABL). High level of T. forsythia was associated also with bleeding on probing (BOP). The combination of the four bacteria, i.e., the bacterial burden index, was associated with moderate to severe periodontitis with an odds ratio (OR) of 2.40 (95% CI 1.39-4.13). When A. actinomycetemcomitans was excluded from the combination of the bacteria, the OR was improved to 2.61 (95% CI 1.51-4.52). The highest OR 3.59 (95% CI 1.94-6.63) was achieved when P. intermedia was further excluded from the combination and only the levels of P. gingivalis and T

  4. Periodontal Bacteria and Prediabetes Prevalence in ORIGINS: The Oral Infections, Glucose Intolerance, and Insulin Resistance Study.

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    Demmer, R T; Jacobs, D R; Singh, R; Zuk, A; Rosenbaum, M; Papapanou, P N; Desvarieux, M

    2015-09-01

    Periodontitis and type 2 diabetes mellitus are known to be associated. The relationship between periodontal microbiota and early diabetes risk has not been studied. We investigated the association between periodontal bacteria and prediabetes prevalence among diabetes-free adults. ORIGINS (the Oral Infections, Glucose Intolerance and Insulin Resistance Study) cross sectionally enrolled 300 diabetes-free adults aged 20 to 55 y (mean ± SD, 34 ± 10 y; 77% female). Prediabetes was defined as follows: 1) hemoglobin A1c values ranging from 5.7% to 6.4% or 2) fasting plasma glucose ranging from 100 to 125 mg/dL. In 1,188 subgingival plaque samples, 11 bacterial species were assessed at baseline, including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, and Actinomyces naeslundii. Full-mouth clinical periodontal examinations were performed, and participants were defined as having no/mild periodontitis vs. moderate/severe periodontitis per the definition of the Centers for Disease Control and Prevention / American Academy of Periodontology. Modified Poisson regression evaluated prediabetes prevalence across bacterial tertiles. Prevalence ratios and 95% confidence intervals for third vs. first tertiles are presented. All analyses were adjusted for cardiometabolic risk factors. All results presented currently arise from the baseline cross section. Prediabetes prevalence was 18%, and 58% of participants had moderate/severe periodontitis. Prevalence ratios (95% confidence intervals) summarizing associations between bacterial levels and prediabetes were as follows: A. actinomycetemcomitans, 2.48 (1.34, 4.58), P = 0.004; P. gingivalis, 3.41 (1.78, 6.58), P = 0.0003; T. denticola, 1.99 (0.992, 4.00), P = 0.052; T. forsythia, 1.95 (1.0, 3.84), P = 0.05; A. naeslundii, 0.46 (0.25, 0.85), P = 0.01. The prevalence ratio for prediabetes among participants with moderate/severe vs. no/mild periodontitis was 1.47 (0.78, 2.74), P

  5. Antimicrobial Constituents of Artemisia afra Jacq. ex Willd. against Periodontal Pathogens.

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    More, Garland; Lall, Namrita; Hussein, Ahmed; Tshikalange, Thilivhali Emmanuel

    2012-01-01

    The phytochemical investigation of an ethanol extract of Artemisia afra led to the isolation of six known compounds, acacetin (1), 12α,4α-dihydroxybishopsolicepolide (2), scopoletin (3), α-amyrin (4), phytol (5), and a pentacyclic triterpenoid betulinic acid (6). The compounds were evaluated for antimicrobial activity against Gram positive (Actinomyces naeslundii, Actinomyces israelii, and Streptococcus mutans), Gram negative bacteria (Prevotella intermedia, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans previously known as Actinobacillus actinomycetemcomitans), and Candida albicans. The crude extract of A. afra inhibited the growth of all tested microbial species at concentration range of 1.6 mg/mL to 25 mg/mL. The compounds 1-6 also showed activity range at 1.0 mg/mL to 0.25 mg/mL. Three best compounds (scopoletin, betulinic acid, and acacetin) which showed good antimicrobial activity were selected for further studies. Cytotoxicity of extract and compounds was determined using the XTT cell proliferation kit. The antioxidant activity of the extract and compounds was done using the DPPH scavenging method. The extract showed good antioxidant activity with an IC(50) value of 22.2 μg/mL. Scopoletin had a strong transformation of the DPPH radical into its reduced form, with an IC(50) value of 1.24 μg/mL which was significant to that of vitamin C (1.22 μg/mL). Acacetin and betulinic acid exhibited a decreased scavenging activity with the IC(50) of 2.39 and 2.42 μg/mL, respectively. The extract and compounds showed moderate toxicity on McCoy fibroblast cell line and scopoletin was relatively nontoxic with an IC(50) value of 132.5 μg/mL. Acacetin and betulinic acid also showed a smooth trend of non-toxic effects with IC(50) values of 35.44 and 30.96 μg/mL. The obtained results in this study confirm the use of A. afra in the treatment of microbial infections. PMID:22693528

  6. Antimicrobial Constituents of Artemisia afra Jacq. ex Willd. against Periodontal Pathogens

    Directory of Open Access Journals (Sweden)

    Garland More

    2012-01-01

    Full Text Available The phytochemical investigation of an ethanol extract of Artemisia afra led to the isolation of six known compounds, acacetin (1, 12α,4α-dihydroxybishopsolicepolide (2, scopoletin (3, α-amyrin (4, phytol (5, and a pentacyclic triterpenoid betulinic acid (6. The compounds were evaluated for antimicrobial activity against Gram positive (Actinomyces naeslundii, Actinomyces israelii, and Streptococcus mutans, Gram negative bacteria (Prevotella intermedia, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans previously known as Actinobacillus actinomycetemcomitans, and Candida albicans. The crude extract of A. afra inhibited the growth of all tested microbial species at concentration range of 1.6 mg/mL to 25 mg/mL. The compounds 1–6 also showed activity range at 1.0 mg/mL to 0.25 mg/mL. Three best compounds (scopoletin, betulinic acid, and acacetin which showed good antimicrobial activity were selected for further studies. Cytotoxicity of extract and compounds was determined using the XTT cell proliferation kit. The antioxidant activity of the extract and compounds was done using the DPPH scavenging method. The extract showed good antioxidant activity with an IC50 value of 22.2 μg/mL. Scopoletin had a strong transformation of the DPPH radical into its reduced form, with an IC50 value of 1.24 μg/mL which was significant to that of vitamin C (1.22 μg/mL. Acacetin and betulinic acid exhibited a decreased scavenging activity with the IC50 of 2.39 and 2.42 μg/mL, respectively. The extract and compounds showed moderate toxicity on McCoy fibroblast cell line and scopoletin was relatively nontoxic with an IC50 value of 132.5 μg/mL. Acacetin and betulinic acid also showed a smooth trend of non-toxic effects with IC50 values of 35.44 and 30.96 μg/mL. The obtained results in this study confirm the use of A. afra in the treatment of microbial infections.

  7. Evaluación de la terapia mecánica periodontal en bolsas profundas: Respuesta clínica y bacteriológica Evaluation of periodontal mechanical therapy in deep pockets: Clinical and bacteriological response

    Directory of Open Access Journals (Sweden)

    G Bazzano

    2012-12-01

    Full Text Available Objetivos: Evaluar la composición microbiológica y los parámetros clínicos de bolsas periodontales ≥5 mm de profundidad al inicio, 1 semana, 3 y 12 meses post raspado y alisado radicular. Materiales y Métodos: Se tomaron registros clínicos y muestras de placa subgingival de 44 sitios de pacientes con diagnóstico de periodontitis crónica. Se identificaron por técnica de Reacción en Cadena de la Polimerasa (PCR patógenos putativos periodontales: Aggregatibacter actinomycetemcomitans (Aa, Porphyromonas gingivalis (Pg, Treponema denticola (Td, Tannerella forsythia (Tf y Prevotella intermedia (Pi. Los pacientes recibieron terapia mecánica periodontal y fueron reevaluados a los 7 días, 3 y 12 meses. Resultados: Luego del tratamiento, todos los parámetros clínicos (Placa Bacteriana, Hemorragia, Supuración, Profundidad al Sondaje y Nivel de Inserción Clínica se redujeron significativamente y los valores obtenidos se mantuvieron hasta los 12 meses. Al inicio, las especies bacterianas prevalentes fueron Pg, presente en 66% de los sitios, Tf (55% y Td (41%. Los sitios más profundos se relacionaron con las asociaciones Tf-Td (6.8 mm y Tf-Td-Pi (7 mm. Post terapia, el número de sitios positivos para Td, Tf y Pg se redujo significativamente. Conclusiones: El raspado y alisado radicular mejoró significativamente los parámetros clínicos y redujo la prevalencia de los patógenos periodontales Pg, Tf y Td en bolsas periodontales profundas. Los resultados obtenidos se mantuvieron hasta los 12 meses. No se detectaron mayores pérdidas de inserción clínica en el 86% de los sitios a 3 meses y en 79% a los 12 meses. Los sitios en los que el tratamiento no fue efectivo en la eliminación de patógenos a los 12 meses desarrollaron mayores profundidades de sondaje.Objectives: To evaluate the microbial composition and clinical parameters of periodontal pockets with probing depth ≥5 mm at baseline, 1 week, 3 and 12 months after scaling and root

  8. Solvability of the Diophantine equations xp + 22m y4 = pk z2 and xp + y2 = pk z4

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    We study the solvability of two classes of Diophantine equations by using some new methods and new results in this paper.Letp be an odd prime and Bn denote nth Bernoulli number.We prove that ifp ≡ 1(mod 4)andp |B(p-1)/2,then the equationxp +22mn4 = pky2,m,n,k ∈ N ,k > 1,gcd(x,py)= 1,and the equationxp +y2 =pkz4,k ∈ N,gcd(x,y)= 1,k > 1,21 y have no integral solutions respectively.

  9. Simultaneous Tracking of 3D Actin and Microtubule Strains in Individual MLO-Y4 Osteocytes under Oscillatory Flow

    OpenAIRE

    Baik, Andrew D.; Qiu, Jun; Hillman, Elizabeth M. C.; Dong, Cheng; Guo, X. Edward

    2013-01-01

    Osteocytes in vivo experience complex fluid shear flow patterns to activate mechanotransduction pathways. The actin and microtubule (MT) cytoskeletons have been shown to play an important role in the osteocyte’s biochemical response to fluid shear loading. The dynamic nature of physiologically relevant fluid flow profiles (i.e., 1 Hz oscillatory flow) impedes the ability to image and study both actin and MT cytoskeletons simultaneously in the same cell with high spatiotemporal resolution. To ...

  10. OSCILLATING FLUID FLOW ACTIVATION OF GAP JUNCTION HEMICHANNELS INDUCES ATP RELEASE FROM MLO-Y4 OSTEOCYTES

    OpenAIRE

    Genetos, Damian C.; Kephart, Curtis J.; Zhang, Yue; Yellowley, Clare E.; Donahue, Henry J.

    2007-01-01

    Mechanical loads are required for optimal bone mass. One mechanism whereby mechanical loads are transduced into localized cellular signals is strain-induced fluid flow through lacunae and canaliculi of bone. Gap junctions (GJ) between osteocytes and osteoblasts provides a mechanism whereby flow-induced signals are detected by osteocytes and transduced to osteoblasts. We have demonstrated the importance of GJ and gap junctional intercellular communication (GJIC) in intracellular calcium and pr...

  11. Simultaneous tracking of 3D actin and microtubule strains in individual MLO-Y4 osteocytes under oscillatory flow.

    Science.gov (United States)

    Baik, Andrew D; Qiu, Jun; Hillman, Elizabeth M C; Dong, Cheng; Guo, X Edward

    2013-02-22

    Osteocytes in vivo experience complex fluid shear flow patterns to activate mechanotransduction pathways. The actin and microtubule (MT) cytoskeletons have been shown to play an important role in the osteocyte's biochemical response to fluid shear loading. The dynamic nature of physiologically relevant fluid flow profiles (i.e., 1Hz oscillatory flow) impedes the ability to image and study both actin and MT cytoskeletons simultaneously in the same cell with high spatiotemporal resolution. To overcome these limitations, a multi-channel quasi-3D microscopy technique was developed to track the actin and MT networks simultaneously under steady and oscillatory flow. Cells displayed high intercellular variability and intracellular cytoskeletal variability in strain profiles. Shear Exz was the predominant strain in both steady and oscillatory flows in the form of viscoelastic creep and elastic oscillations, respectively. Dramatic differences were seen in oscillatory flow, however. The actin strains displayed an oscillatory strain profile more often than the MT networks in all the strains tested and had a higher peak-to-trough strain magnitude. Taken together, the actin networks are the more responsive cytoskeletal networks in osteocytes under oscillatory flow and may play a bigger role in mechanotransduction pathway activation and regulation. PMID:23352617

  12. Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging.

    Science.gov (United States)

    Ebersole, Jeffrey L; Kirakodu, Sreenatha S; Novak, M John; Orraca, Luis; Martinez, Janis Gonzalez; Cunningham, Larry L; Thomas, Mark V; Stromberg, Arnold; Pandruvada, Subramanya N; Gonzalez, Octavio A

    2016-01-01

    Evidence has shown activation of T and B cells in gingival tissues in experimental models and in humans diagnosed with periodontitis. The results of this adaptive immune response are noted both locally and systemically with antigenic specificity for an array of oral bacteria, including periodontopathic species, e.g., Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. It has been recognized through epidemiological studies and clinical observations that the prevalence of periodontitis increases with age. This report describes our studies evaluating gingival tissue transcriptomes in humans and specifically exploiting the use of a non-human primate model of naturally occurring periodontitis to delineate gingival mucosal tissue gene expression profiles focusing on cells/genes critical for the development of humoral adaptive immune responses. Patterns of B cell and plasmacyte genes were altered in aging healthy gingival tissues. Substantial increases in a large number of genes reflecting antigen-dependent activation, B cell activation, B cell proliferation, and B cell differentiation/maturation were observed in periodontitis in adults and aged animals. Finally, evaluation of the relationship of these gene expression patterns with those of various tissue destructive molecules (MMP2, MMP9, CTSK, TNFα, and RANKL) showed a greater frequency of positive correlations in healthy tissues versus periodontitis tissues, with only MMP9 correlations similar between the two tissue types. These results are consistent with B cell response activities in healthy tissues potentially contributing to muting the effects of the tissue destructive biomolecules, whereas with periodontitis this relationship is adversely affected and enabling a progression of tissue destructive events. PMID:27486459

  13. Antimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjects.

    Science.gov (United States)

    Mariano, F S; Campanelli, A P; Nociti Jr, F H; Mattos-Graner, R O; Gonçalves, R B

    2012-11-01

    Neutrophils play an important role in periodontitis by producing nitric oxide (NO) and antimicrobial peptides, molecules with microbicidal activity via oxygen-dependent and -independent mechanisms, respectively. It is unknown whether variation in the production of antimicrobial peptides such as LL-37, human neutrophil peptides (HNP) 1-3, and NO by neutrophils influences the pathogenesis of periodontal diseases. We compared the production of these peptides and NO by lipopolysaccharide (LPS)-stimulated neutrophils isolated from healthy subjects and from patients with periodontitis. Peripheral blood neutrophils were cultured with or without Aggregatibacter actinomycetemcomitans-LPS (Aa-LPS), Porphyromonas gingivalis-LPS (Pg-LPS) and Escherichia coli-LPS (Ec-LPS). qRT-PCR was used to determine quantities of HNP 1-3 and LL-37 mRNA in neutrophils. Amounts of HNP 1-3 and LL-37 proteins in the cell culture supernatants were also determined by ELISA. In addition, NO levels in neutrophil culture supernatants were quantitated by the Griess reaction. Neutrophils from periodontitis patients cultured with Aa-LPS, Pg-LPS and Ec-LPS expressed higher HNP 1-3 mRNA than neutrophils from healthy subjects. LL-37 mRNA expression was higher in neutrophils from patients stimulated with Aa-LPS. Neutrophils from periodontitis patients produced significantly higher LL-37 protein levels than neutrophils from healthy subjects when stimulated with Pg-LPS and Ec-LPS, but no difference was observed in HNP 1-3 production. Neutrophils from periodontitis patients cultured or not with Pg-LPS and Ec-LPS produced significantly lower NO levels than neutrophils from healthy subjects. The significant differences in the production of LL-37 and NO between neutrophils from healthy and periodontitis subjects indicate that production of these molecules might influence individual susceptibility to important periodontal pathogens.

  14. Periowave demonstrates bactericidal activity against periopathogens and leads to improved clinical outcomes in the treatment of adult periodontitis

    Science.gov (United States)

    Street, Cale N.; Andersen, Roger; Loebel, Nicolas G.

    2009-02-01

    Periodontitis affects half of the U.S. population over 50, and is the leading cause of tooth loss after 35. It is believed to be caused by growth of complex bacterial biofilms on the tooth surface below the gumline. Photodynamic therapy, a technology used commonly in antitumor applications, has more recently been shown to exhibit antimicrobial efficacy. We have demonstrated eradication of the periopathogens Porphyromonas gingivalis, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans in vitro using PeriowaveTM; a commercial photodisinfection system. In addition, several clinical studies have now demonstrated the efficacy of this treatment. A pilot study in the U.S. showed that 68% of patients treated with PeriowaveTM adjunctively to scaling and root planing (SRP) showed clinical attachment level increase of >1 mm, as opposed to 30% with SRP alone. In a subsequent larger study, a second PeriowaveTM treatment 6 weeks after initial treatment led to pocket depth improvements of >1.5 mm in 89% of patients. Finally, in the most recent multicenter, randomized, examiner-blinded study conducted on 121 subjects in Canada, PeriowaveTM treatment produced highly significant gains in attachment level (0.88 mm vs. 0.57 mm; p=0.003) and pocket depth (0.87 mm vs. 0.63 mm; p=0.01) as compared to SRP alone. In summary, PeriowaveTM demonstrated strong bactericidal activity against known periopathogens, and treatment of periodontitis using this system produced significantly better clinical outcomes than SRP alone. This, along with the absence of any adverse events in patients treated to date demonstrates that PDT is a safe and effective treatment for adult chronic periodontitis.

  15. Subgingival bacterial colonization profiles correlate with gingival tissue gene expression

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    Handfield Martin

    2009-10-01

    Full Text Available Abstract Background Periodontitis is a chronic inflammatory disease caused by the microbiota of the periodontal pocket. We investigated the association between subgingival bacterial profiles and gene expression patterns in gingival tissues of patients with periodontitis. A total of 120 patients undergoing periodontal surgery contributed with a minimum of two interproximal gingival papillae (range 2-4 from a maxillary posterior region. Prior to tissue harvesting, subgingival plaque samples were collected from the mesial and distal aspects of each tissue sample. Gingival tissue RNA was extracted, reverse-transcribed, labeled, and hybridized with whole-genome microarrays (310 in total. Plaque samples were analyzed using checkerboard DNA-DNA hybridizations with respect to 11 bacterial species. Random effects linear regression models considered bacterial levels as exposure and expression profiles as outcome variables. Gene Ontology analyses summarized the expression patterns into biologically relevant categories. Results Wide inter-species variation was noted in the number of differentially expressed gingival tissue genes according to subgingival bacterial levels: Using a Bonferroni correction (p -7, 9,392 probe sets were differentially associated with levels of Tannerella forsythia, 8,537 with Porphyromonas gingivalis, 6,460 with Aggregatibacter actinomycetemcomitans, 506 with Eikenella corrodens and only 8 with Actinomyces naeslundii. Cluster analysis identified commonalities and differences among tissue gene expression patterns differentially regulated according to bacterial levels. Conclusion Our findings suggest that the microbial content of the periodontal pocket is a determinant of gene expression in the gingival tissues and provide new insights into the differential ability of periodontal species to elicit a local host response.

  16. Effect of periodontal pathogens on the metatranscriptome of a healthy multispecies biofilm model.

    Science.gov (United States)

    Frias-Lopez, Jorge; Duran-Pinedo, Ana

    2012-04-01

    Oral bacterial biofilms are highly complex microbial communities with up to 700 different bacterial taxa. We report here the use of metatranscriptomic analysis to study patterns of community gene expression in a multispecies biofilm model composed of species found in healthy oral biofilms (Actinomyces naeslundii, Lactobacillus casei, Streptococcus mitis, Veillonella parvula, and Fusobacterium nucleatum) and the same biofilm plus the periodontopathogens Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. The presence of the periodontopathogens altered patterns in gene expression, and data indicate that transcription of protein-encoding genes and small noncoding RNAs is stimulated. In the healthy biofilm hypothetical proteins, transporters and transcriptional regulators were upregulated while chaperones and cell division proteins were downregulated. However, when the pathogens were present, chaperones were highly upregulated, probably due to increased levels of stress. We also observed a significant upregulation of ABC transport systems and putative transposases. Changes in Clusters of Orthologous Groups functional categories as well as gene set enrichment analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways showed that in the absence of pathogens, only sets of proteins related to transport and secondary metabolism were upregulated, while in the presence of pathogens, proteins related to growth and division as well as a large portion of transcription factors were upregulated. Finally, we identified several small noncoding RNAs whose predicted targets were genes differentially expressed in the open reading frame libraries. These results show the importance of pathogens controlling gene expression of a healthy oral community and the usefulness of metatranscriptomic techniques to study gene expression profiles in complex microbial community models. PMID:22328675

  17. Prevalence of periodontopathogenic bacteria in patients suffering from periodontitis using culture and PCR methods

    Directory of Open Access Journals (Sweden)

    Amir Aliramezani

    2012-01-01

    Full Text Available Background and Aims: Periodontitis is one of the most common oral diseases with the various incidence rates in different populations. A number of bacteria are considered as the major etiologic agents of periodontitis. The aim of the present study was to determine the prevalence of periodontopathogen bacteria in patients using both PCR and culture techniques.Materials and Methods: In this study, one-hundred patients (including 62 females and 38 males with an average age of 49±11.5 years with adult periodontitis referred to periodontics department of School of Dentistry/Tehran University of Medical Sciences were investigated. The samples were taken and sent immediately to the laboratory for culture and molecular evaluation. The PCR was performed using specific primers and the statistical analysis of data was performed using SPSS statistic software and McNemar test.Results: The results demonstrated that the total detection rate in culture method was 64%. The rate of Aggregatibacter actinomycetemcomitans (Aa was 28% which was significantly higher than that of Porphyromonas gingivalis (Pg (6% and Prevotella intermedia (Pi (3%. 27% of cases showed mixed bacterial growth. 65% of patients were positive using molecular method. The rate of Aa (30% was significantly higher than that of Pg (7% and Pi (5%. The mixed PCR positive rate containing of Aa, Pg and Pi was (23%.Conclusion: In this study, it was found that most of the bacteria isolated using culture and molecular methods were Aa, Pg and Pi, respectively. Although the detection frequencies of both techniques were similar, the specificity, sensitivity and bacterial detection speed of the PCR technique is obviously higher. Therefore, the use of molecular techniques is strongly recommended. However, both techniques seem to be suitable for microbiological diagnostics.

  18. Host response mechanisms in periodontal diseases

    Directory of Open Access Journals (Sweden)

    Nora SILVA

    2015-06-01

    Full Text Available Periodontal diseases usually refer to common inflammatory disorders known as gingivitis and periodontitis, which are caused by a pathogenic microbiota in the subgingival biofilm, including Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia and Treponema denticola that trigger innate, inflammatory, and adaptive immune responses. These processes result in the destruction of the tissues surrounding and supporting the teeth, and eventually in tissue, bone and finally, tooth loss. The innate immune response constitutes a homeostatic system, which is the first line of defense, and is able to recognize invading microorganisms as non-self, triggering immune responses to eliminate them. In addition to the innate immunity, adaptive immunity cells and characteristic cytokines have been described as important players in the periodontal disease pathogenesis scenario, with a special attention to CD4+ T-cells (T-helper cells. Interestingly, the T cell-mediated adaptive immunity development is highly dependent on innate immunity-associated antigen presenting cells, which after antigen capture undergo into a maturation process and migrate towards the lymph nodes, where they produce distinct patterns of cytokines that will contribute to the subsequent polarization and activation of specific T CD4+ lymphocytes. Skeletal homeostasis depends on a dynamic balance between the activities of the bone-forming osteoblasts (OBLs and bone-resorbing osteoclasts (OCLs. This balance is tightly controlled by various regulatory systems, such as the endocrine system, and is influenced by the immune system, an osteoimmunological regulation depending on lymphocyte- and macrophage-derived cytokines. All these cytokines and inflammatory mediators are capable of acting alone or in concert, to stimulate periodontal breakdown and collagen destruction via tissue-derived matrix metalloproteinases, a characterization of the progression of periodontitis as

  19. D-Galactose as an autoinducer 2 inhibitor to control the biofilm formation of periodontopathogens.

    Science.gov (United States)

    Ryu, Eun-Ju; Sim, Jaehyun; Sim, Jun; Lee, Julian; Choi, Bong-Kyu

    2016-09-01

    Autoinducer 2 (AI-2) is a quorum sensing molecule to which bacteria respond to regulate various phenotypes, including virulence and biofilm formation. AI-2 plays an important role in the formation of a subgingival biofilm composed mostly of Gram-negative anaerobes, by which periodontitis is initiated. The aim of this study was to evaluate D-galactose as an inhibitor of AI-2 activity and thus of the biofilm formation of periodontopathogens. In a search for an AI-2 receptor of Fusobacterium nucleatum, D-galactose binding protein (Gbp, Gene ID FN1165) showed high sequence similarity with the ribose binding protein (RbsB), a known AI-2 receptor of Aggregatibacter actinomycetemcomitans. D-Galactose was evaluated for its inhibitory effect on the AI-2 activity of Vibrio harveyi BB152 and F. nucleatum, the major coaggregation bridge organism, which connects early colonizing commensals and late pathogenic colonizers in dental biofilms. The inhibitory effect of D-galactose on the biofilm formation of periodontopathogens was assessed by crystal violet staining and confocal laser scanning microscopy in the absence or presence of AI-2 and secreted molecules of F. nucleatum. D-Galactose significantly inhibited the AI-2 activity of V. harveyi and F. nucleatum. In addition, D-galactose markedly inhibited the biofilm formation of F. nucleatum, Porphyromonas gingivalis, and Tannerella forsythia induced by the AI-2 of F. nucleatum without affecting bacterial growth. Our results demonstrate that the Gbp may function as an AI-2 receptor and that galactose may be used for prevention of the biofilm formation of periodontopathogens by targeting AI-2 activity. PMID:27572513

  20. The periodontal health of lead-exposed children living in a shipyard industrial area.

    Science.gov (United States)

    Youravong, Nattaporn; Teanpaisan, Rawee

    2015-05-01

    In a cross-sectional design, 292 schoolchildren living around a shipyard area, known to be contaminated with lead from shipyard industry, were examined to verify the association between lead exposure and periodontal health. The probing pocket depth (PD), bleeding on probing, plaque and calculus, and the presence of Aggregatibacter actinomycetemcomitans (Aa) in subgingival crevices were recorded. Gingival inflammation was the most common (98%) among children in the area. No significant difference in gingival inflammation was observed between high blood lead (PbB) and low PbB children. The prevalence rate of probing PD of ≥5 mm was 14%. The high PbB group showed more deep pockets at tooth 16 (upper right first permanent molar) and tooth 46 (lower right first permanent molar) than the low PbB group. The odds ratios (ORs) for having probing PD ≥5 mm after adjusting for other factors were 3.63 (95% confidence interval (CI), 1.24-10.61; p = 0.02) for tooth 16 and 3.93 (95% CI, 1.18-13.00; p = 0.02) for tooth 46. The presence of Aa was observed in 17% of the children and it significantly increased in high PbB compared with low PbB children at tooth 46 (OR = 5.53, 95% CI: 1.68-18.15; p = 0.005). This study may suggest no association between lead exposure and gingival inflammation, yet there was the involvement of deeper periodontal tissue in lead-exposed children.

  1. Dose-response met-RANTES treatment of experimental periodontitis: a narrow edge between the disease severity attenuation and infection control.

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Repeke

    Full Text Available Chemokines and chemokine receptors have been implicated in the selective migration of leukocyte subsets to periodontal tissues, which consequently influences the disease outcome. Among these chemoattractants, the chemokines CCL3, CCL4 and CCL5 and its receptors, CCR1 and CCR5, have been associated with increased disease severity in mice and humans. Therefore, in this study we investigated the modulation of experimental periodontitis outcome by the treatment with a specific antagonist of CCR1 and 5 receptors, called met-RANTES. C57Bl/6 mice was orally infected with Aggregatibacter actinomycetemcomitans and treated with 0.05, 0.1, 0.5, 1.5 and 5 mg doses of met-RANTES on alternate days, and evaluated by morphometric, cellular, enzymatic and molecular methods. At 0.5 mg up to 5 mg doses, a strong reduction in the alveolar bone loss and inflammatory cell migration were observed. Interestingly, 5 mg dose treatment resulted in the maximum inhibition of inflammatory cell migration, but resulted in a similar inhibition of bone loss when compared with the lower doses, and also resulted in increased bacterial load and CRP response. When 0.5 and 5 mg therapy regimens were compared it was observed that both therapeutic protocols were able to downregulate the levels of pro-inflammatory, Th1-type and osteoclastogenic cytokines, and CD3+ and F4/80+ cells migration to periodontal tissues, but the high dose modulates host response in a more pronounced and unspecific and excessive way, interfering also with the production of antimicrobial mediators such as MPO, iNOS and IgG, and with GR1+ and CD19+ cells migration. Our results demonstrate a thin line between beneficial immunoregulation and impaired host defense during experimental periodontitis, and the determination of the exact equilibrium point is mandatory for the improvement of immune-targeted therapy of periodontitis.

  2. Clinical status and detection of periodontopathogens and Streptococcus mutans in children with high levels of supragingival biofilm

    Directory of Open Access Journals (Sweden)

    Sheila Cavalca Cortelli

    2009-09-01

    Full Text Available Knowledge about the presence of some important oral pathogens is an important step in better identifying children at risk for periodontal and/or caries diseases in later life. The purpose of this study was to detect the presence of Streptococcus mutans (Sm, Aggregatibacter actinomycetemcomitans (Aa, Campylobacter rectus (Cr, Porphyromonas gingivalis (Pg, Prevotella intermedia (Pi, and Tannerella forsythia (Tf in gingival biofilm samples from 196 children, and to assess whether any of these pathogens are more associated with gingival inflammation extension and the Decayed/Missing/Filled teeth (DMFT/dmft index. The subjects presented plaque index greater than 80% and were divided in 3 groups according to the bleeding index (BI: I Low bleeding ( 60%. The presence of each pathogen was determined by PCR. The prevalence of Sm was 71.9% and the mean dmft/DMFT was 6.68. The prevalence in low, medium and high bleeding groups was 43.5%, 34.5% and 46.7% for Aa; 43.5%, 37.9%, and 36.7% for Cr; 99.1%, 100%, and 96.7% for Pg; 56.5%, 56.9%, and 66.7% for Pi; and 58.3%, 60.3%, and 56.7% for Tf, respectively. Pg (99.0% was the most prevalent periodontal pathogen detected followed by Tf (58.7%, Pi (58.2%, Aa (41.3% and Cr (40.8%. Our study indicated that in this high plaque index population studied, a high prevalence of Sm and high mean DMFT were observed. In addition, the presence of Pi was associated with the presence of inflammation (P < 0.05 whereas Cr was associated with periodontal health (P < 0.05.

  3. Role and regulation of the Flp/Tad pilus in the virulence of Pectobacterium atrosepticum SCRI1043 and Pectobacterium wasabiae SCC3193.

    Directory of Open Access Journals (Sweden)

    Johanna Nykyri

    Full Text Available In this study, we characterized a putative Flp/Tad pilus-encoding gene cluster, and we examined its regulation at the transcriptional level and its role in the virulence of potato pathogenic enterobacteria of the genus Pectobacterium. The Flp/Tad pilus-encoding gene clusters in Pectobacterium atrosepticum, Pectobacterium wasabiae and Pectobacterium aroidearum were compared to previously characterized flp/tad gene clusters, including that of the well-studied Flp/Tad pilus model organism Aggregatibacter actinomycetemcomitans, in which this pilus is a major virulence determinant. Comparative analyses revealed substantial protein sequence similarity and open reading frame synteny between the previously characterized flp/tad gene clusters and the cluster in Pectobacterium, suggesting that the predicted flp/tad gene cluster in Pectobacterium encodes a Flp/Tad pilus-like structure. We detected genes for a novel two-component system adjacent to the flp/tad gene cluster in Pectobacterium, and mutant analysis demonstrated that this system has a positive effect on the transcription of selected Flp/Tad pilus biogenesis genes, suggesting that this response regulator regulate the flp/tad gene cluster. Mutagenesis of either the predicted regulator gene or selected Flp/Tad pilus biogenesis genes had a significant impact on the maceration ability of the bacterial strains in potato tubers, indicating that the Flp/Tad pilus-encoding gene cluster represents a novel virulence determinant in Pectobacterium. Soft-rot enterobacteria in the genera Pectobacterium and Dickeya are of great agricultural importance, and an investigation of the virulence of these pathogens could facilitate improvements in agricultural practices, thus benefiting farmers, the potato industry and consumers.

  4. Role and regulation of the Flp/Tad pilus in the virulence of Pectobacterium atrosepticum SCRI1043 and Pectobacterium wasabiae SCC3193.

    Science.gov (United States)

    Nykyri, Johanna; Mattinen, Laura; Niemi, Outi; Adhikari, Satish; Kõiv, Viia; Somervuo, Panu; Fang, Xin; Auvinen, Petri; Mäe, Andres; Palva, E Tapio; Pirhonen, Minna

    2013-01-01

    In this study, we characterized a putative Flp/Tad pilus-encoding gene cluster, and we examined its regulation at the transcriptional level and its role in the virulence of potato pathogenic enterobacteria of the genus Pectobacterium. The Flp/Tad pilus-encoding gene clusters in Pectobacterium atrosepticum, Pectobacterium wasabiae and Pectobacterium aroidearum were compared to previously characterized flp/tad gene clusters, including that of the well-studied Flp/Tad pilus model organism Aggregatibacter actinomycetemcomitans, in which this pilus is a major virulence determinant. Comparative analyses revealed substantial protein sequence similarity and open reading frame synteny between the previously characterized flp/tad gene clusters and the cluster in Pectobacterium, suggesting that the predicted flp/tad gene cluster in Pectobacterium encodes a Flp/Tad pilus-like structure. We detected genes for a novel two-component system adjacent to the flp/tad gene cluster in Pectobacterium, and mutant analysis demonstrated that this system has a positive effect on the transcription of selected Flp/Tad pilus biogenesis genes, suggesting that this response regulator regulate the flp/tad gene cluster. Mutagenesis of either the predicted regulator gene or selected Flp/Tad pilus biogenesis genes had a significant impact on the maceration ability of the bacterial strains in potato tubers, indicating that the Flp/Tad pilus-encoding gene cluster represents a novel virulence determinant in Pectobacterium. Soft-rot enterobacteria in the genera Pectobacterium and Dickeya are of great agricultural importance, and an investigation of the virulence of these pathogens could facilitate improvements in agricultural practices, thus benefiting farmers, the potato industry and consumers. PMID:24040039

  5. Early colonization of the oral cavity in 6- and 12-month-old infants by cariogenic and periodontal pathogens: a case-control study.

    Science.gov (United States)

    Merglova, Vlasta; Polenik, Pavel

    2016-09-01

    The colonization of the oral cavity by cariogenic and periodontal pathogens occurs earlier than previously thought. This study aimed to identify the presence and quantity of representative cariogenic and periodontal pathogens in the oral cavities of 6- and 12-month olds and to evaluate the influence of C-section delivery on early Streptococcus mutans (Sm) colonization of the oral cavity. The research cohort was composed of 59 infants (35 infants were delivered vaginally and 24 via C-section) and their mothers. At 6 months of age, the infants were examined, and unstimulated saliva samples were collected. Variables concerning mothers were DMF index and salivary levels of Sm. Repeated saliva samples were taken 6 months later. The representative cariogenic and periodontal microorganisms were identified, and their quantities were measured using a polymerase chain reaction-based method. The relationships between the presence of detected microbes, the mode of delivery, and maternal variables were evaluated using paired t tests, chi-squared test, and ANOVAs. High rates of cariogenic bacteria, Aggregatibacter actinomycetemcomitans (Aa) and Fusobacterium nucleatum (Fn), were found in both infant cohorts. An analysis of the differences between delivery methods revealed that the group of 6-month-old vaginally delivered infants had a significantly higher amount of Sm. We conclude that the cariogenic bacteria, Aa and Fn, are present in edentulous infants. This presence increases in the months following the eruption of the deciduous teeth. Results did not confirm the influence of C-section delivery on the early Sm colonization of the oral cavity. PMID:26914065

  6. Frequency of periodontal pathogens and Helicobacter pylori in the mouths and stomachs of obese individuals submitted to bariatric surgery: a cross-sectional study

    Science.gov (United States)

    PATARO, André Luiz; CORTELLI, Sheila Cavalca; ABREU, Mauro Henrique Nogueira Guimarães; CORTELLI, José Roberto; FRANCO, Gilson Cesar Nobre; AQUINO, Davi Romeiro; COTA, Luis Otavio Miranda; COSTA, Fernando Oliveira

    2016-01-01

    ABSTRACT Objectives This cross-sectional study compared the frequency of oral periodontopathogens and H. pylori in the mouths and stomachs of obese individuals with or without periodontitis submitted to bariatric surgery. Material and Methods One hundred and fifty-four men and women aged 18-65 were conveniently distributed into four groups. Two groups were composed of individuals who underwent bariatric surgery with (BP) (n=40) and without (BNP) (n=39) periodontitis and two obese control groups with (CP) (n=35) and without (CNP) (n=40) periodontitis. The oral pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Parvimonas micra, Treponema denticola, Tannerella forsythia, Campylobacter rectus, and Helicobacter pylori were detected by a polymerase chain reaction technique using saliva, tongue and stomach biopsy samples. Results Statistical analysis demonstrated that periodontopathogens were highly frequent in the mouth (up to 91.4%). In the bariatric surgically treated group, orally, P. gingivalis, T. denticola and T. forsythia were more frequent in periodontitis, while C. rectus was more frequent in non-periodontitis subjects. Stomach biopsies also revealed the high frequency of five oral species in both candidates for bariatric surgery (91.6%) and the bariatric (83.3%) groups. H. pylori was frequently detected in the mouth (50.0%) and stomach (83.3%). In the stomach, oral species and H. pylori appeared in lower frequency in the bariatric group. Conclusions Obese individuals showed high frequencies of periodontopathogens and H. pylori in their mouths and stomachs. Bariatric surgery showed an inverse microbial effect on oral and stomach environments by revealing higher oral and lower stomach bacterial frequencies. PMID:27383704

  7. Are Putative Periodontal Pathogens Reliable Diagnostic Markers?▿

    Science.gov (United States)

    Riep, Birgit; Edesi-Neuß, Lilian; Claessen, Friderike; Skarabis, Horst; Ehmke, Benjamin; Flemmig, Thomas F.; Bernimoulin, Jean-Pierre; Göbel, Ulf B.; Moter, Annette

    2009-01-01

    Periodontitis is one of the most common chronic inflammatory diseases. A number of putative bacterial pathogens have been associated with the disease and are used as diagnostic markers. In the present study, we compared the prevalence of oral bacterial species in the subgingival biofilm of generalized aggressive periodontitis (GAP) (n = 44) and chronic periodontitis (CP) (n = 46) patients with that of a periodontitis-resistant control group (PR) (n = 21). The control group consisted of subjects at least 65 years of age with only minimal or no periodontitis and no history of periodontal treatment. A total of 555 samples from 111 subjects were included in this study. The samples were analyzed by PCR of 16S rRNA gene fragments and subsequent dot blot hybridization using oligonucleotide probes specific for Aggregatibacter (Actinobacillus) actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, a Treponema denticola-like phylogroup (Treponema phylogroup II), Treponema lecithinolyticum, Campylobacter rectus, Fusobacterium spp., and Fusobacterium nucleatum, as well as Capnocytophaga ochracea. Our data confirm a high prevalence of the putative periodontal pathogens P. gingivalis, P. intermedia, and T. forsythia in the periodontitis groups. However, these species were also frequently detected in the PR group. For most of the species tested, the prevalence was more associated with increased probing depth than with the subject group. T. lecithinolyticum was the only periodontopathogenic species showing significant differences both between GAP and CP patients and between GAP patients and PR subjects. C. ochracea was associated with the PR subjects, regardless of the probing depth. These results indicate that T. lecithinolyticum may be a diagnostic marker for GAP and C. ochracea for periodontal health. They also suggest that current presumptions of the association of specific bacteria with periodontal health and disease require further

  8. Macrophage inflammatory protein-1α shows predictive value as a risk marker for subjects and sites vulnerable to bone loss in a longitudinal model of aggressive periodontitis.

    Directory of Open Access Journals (Sweden)

    Daniel H Fine

    Full Text Available Improved diagnostics remains a fundamental goal of biomedical research. This study was designed to assess cytokine biomarkers that could predict bone loss (BL in localized aggressive periodontitis. 2,058 adolescents were screened. Two groups of 50 periodontally healthy adolescents were enrolled in the longitudinal study. One group had Aggregatibacter actinomycetemcomitans (Aa, the putative pathogen, while the matched cohort did not. Cytokine levels were assessed in saliva and gingival crevicular fluid (GCF. Participants were sampled, examined, and radiographed every 6 months for 2-3 years. Disease was defined as radiographic evidence of BL. Saliva and GCF was collected at each visit, frozen, and then tested retrospectively after detection of BL. Sixteen subjects with Aa developed BL. Saliva from Aa-positive and Aa-negative healthy subjects was compared to subjects who developed BL. GCF was collected from 16 subjects with BL and from another 38 subjects who remained healthy. GCF from BL sites in the 16 subjects was compared to healthy sites in these same subjects and to healthy sites in subjects who remained healthy. Results showed that cytokines in saliva associated with acute inflammation were elevated in subjects who developed BL (i.e., MIP-1α MIP-1β IL-α, IL-1β and IL-8; p<0.01. MIP-1α was elevated 13-fold, 6 months prior to BL. When MIP-1α levels were set at 40 pg/ml, 98% of healthy sites were below that level (Specificity; whereas, 93% of sites with BL were higher (Sensitivity, with comparable Predictive Values of 98%; p<0.0001; 95% C.I. = 42.5-52.7. MIP-1α consistently showed elevated levels as a biomarker for BL in both saliva and GCF, 6 months prior to BL. MIP-1α continues to demonstrate its strong candidacy as a diagnostic biomarker for both subject and site vulnerability to BL.

  9. Magnolol Ameliorates Ligature-Induced Periodontitis in Rats and Osteoclastogenesis: In Vivo and In Vitro Study

    Directory of Open Access Journals (Sweden)

    Sheng-Hua Lu

    2013-01-01

    Full Text Available Periodontal disease characterized by alveolar bone resorption and bacterial pathogen-evoked inflammatory response has been believed to have an important impact on human oral health. The aim of this study was to evaluate whether magnolol, a main constituent of Magnolia officinalis, could inhibit the pathological features in ligature-induced periodontitis in rats and osteoclastogenesis. The sterile, 3–0 (diameter; 0.2 mm black braided silk thread, was placed around the cervix of the upper second molars bilaterally and knotted medially to induce periodontitis. The morphological changes around the ligated molars and alveolar bone were examined by micro-CT. The distances between the amelocemental junction and the alveolar crest of the upper second molars bilaterally were measured to evaluate the alveolar bone loss. Administration of magnolol (100 mg/kg, p.o. significantly inhibited alveolar bone resorption, the number of osteoclasts on bony surface, and protein expression of receptor activator of nuclear factor-κB ligand (RANKL, a key mediator promoting osteoclast differentiation, in ligated rats. Moreover, the ligature-induced neutrophil infiltration, expression of inducible nitric oxide synthase, cyclooxygenase-2, matrix metalloproteinase (MMP-1 and MMP-9, superoxide formation, and nuclear factor-κB activation in inflamed gingival tissues were all attenuated by magnolol. In the in vitro study, magnolol also inhibited the growth of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans that are key pathogens initiating periodontal disease. Furthermore, magnolol dose dependently reduced RANKL-induced osteoclast differentiation from RAW264.7 macrophages, tartrate-resistant acid phosphatase (TRAP activity of differentiated cells accompanied by a significant attenuation of resorption pit area caused by osteoclasts. Collectively, we demonstrated for the first time that magnolol significantly ameliorates the alveolar bone loss in

  10. Quantification of key periodontal pathogens in insulin-dependent type 2 diabetic and non-diabetic patients with generalized chronic periodontitis.

    Science.gov (United States)

    Aemaimanan, Piyamas; Amimanan, Piyawan; Taweechaisupapong, Suwimol

    2013-08-01

    Periodontitis is a common problem in patients with diabetes mellitus (DM), however, differences in the putative periodontal pathogens in subjects with DM compared to non-DM subjects are still inconclusive. The red complex, which includes Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, encompasses the most important pathogens in adult periodontal disease. The aim of the present study was to compare cell numbers of P. gingivalis, T. denticola, T. forsythia and Aggregatibacter actinomycetemcomitans in gingival sulcus of healthy, gingivitis and periodontitis sites of non-diabetes mellitus (NDM), controlled and poorly controlled insulin-dependent DM (CDM and PDM) patients with generalized chronic periodontitis. Subgingival plaque samples were collected from 19 CDM, 19 PDM and 19 NDM patients. Taqman real time-PCR was used to determine bacterial cell number. At subject level, the quantity of red complex bacteria was significantly higher in PDM than those of NDM and positively correlated with HbA1c. At site level (total 342 sites), cell numbers of T. denticola and T. forsythia in healthy sites of CDM and PDM were significantly higher than those of NDM. In gingivitis sites, the numbers of P. gingivalis in CDM and PDM and T. forsythia in PDM were significantly higher than those of NDM while in periodontitis sites, higher quantity of P. gingivalis in PDM was observed. Our study indicated that poor glycemic control is associated with increasing cell numbers of red complex bacteria in subgingival biofilm. PMID:23827459

  11. Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging

    Science.gov (United States)

    Ebersole, Jeffrey L.; Kirakodu, Sreenatha S.; Novak, M. John; Orraca, Luis; Martinez, Janis Gonzalez; Cunningham, Larry L.; Thomas, Mark V.; Stromberg, Arnold; Pandruvada, Subramanya N.; Gonzalez, Octavio A.

    2016-01-01

    Evidence has shown activation of T and B cells in gingival tissues in experimental models and in humans diagnosed with periodontitis. The results of this adaptive immune response are noted both locally and systemically with antigenic specificity for an array of oral bacteria, including periodontopathic species, e.g., Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. It has been recognized through epidemiological studies and clinical observations that the prevalence of periodontitis increases with age. This report describes our studies evaluating gingival tissue transcriptomes in humans and specifically exploiting the use of a non-human primate model of naturally occurring periodontitis to delineate gingival mucosal tissue gene expression profiles focusing on cells/genes critical for the development of humoral adaptive immune responses. Patterns of B cell and plasmacyte genes were altered in aging healthy gingival tissues. Substantial increases in a large number of genes reflecting antigen-dependent activation, B cell activation, B cell proliferation, and B cell differentiation/maturation were observed in periodontitis in adults and aged animals. Finally, evaluation of the relationship of these gene expression patterns with those of various tissue destructive molecules (MMP2, MMP9, CTSK, TNFα, and RANKL) showed a greater frequency of positive correlations in healthy tissues versus periodontitis tissues, with only MMP9 correlations similar between the two tissue types. These results are consistent with B cell response activities in healthy tissues potentially contributing to muting the effects of the tissue destructive biomolecules, whereas with periodontitis this relationship is adversely affected and enabling a progression of tissue destructive events. PMID:27486459

  12. Antimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjects

    Directory of Open Access Journals (Sweden)

    F.S. Mariano

    2012-11-01

    Full Text Available Neutrophils play an important role in periodontitis by producing nitric oxide (NO and antimicrobial peptides, molecules with microbicidal activity via oxygen-dependent and -independent mechanisms, respectively. It is unknown whether variation in the production of antimicrobial peptides such as LL-37, human neutrophil peptides (HNP 1-3, and NO by neutrophils influences the pathogenesis of periodontal diseases. We compared the production of these peptides and NO by lipopolysaccharide (LPS-stimulated neutrophils isolated from healthy subjects and from patients with periodontitis. Peripheral blood neutrophils were cultured with or without Aggregatibacter actinomycetemcomitans-LPS (Aa-LPS, Porphyromonas gingivalis-LPS (Pg-LPS and Escherichia coli-LPS (Ec-LPS. qRT-PCR was used to determine quantities of HNP 1-3 and LL-37 mRNA in neutrophils. Amounts of HNP 1-3 and LL-37 proteins in the cell culture supernatants were also determined by ELISA. In addition, NO levels in neutrophil culture supernatants were quantitated by the Griess reaction. Neutrophils from periodontitis patients cultured with Aa-LPS, Pg-LPS and Ec-LPS expressed higher HNP 1-3 mRNA than neutrophils from healthy subjects. LL-37 mRNA expression was higher in neutrophils from patients stimulated with Aa-LPS. Neutrophils from periodontitis patients produced significantly higher LL-37 protein levels than neutrophils from healthy subjects when stimulated with Pg-LPS and Ec-LPS, but no difference was observed in HNP 1-3 production. Neutrophils from periodontitis patients cultured or not with Pg-LPS and Ec-LPS produced significantly lower NO levels than neutrophils from healthy subjects. The significant differences in the production of LL-37 and NO between neutrophils from healthy and periodontitis subjects indicate that production of these molecules might influence individual susceptibility to important periodontal pathogens.

  13. Identification of αLβ2, αMβ2, and αXβ2 integrins as receptors for Actinobacillus actinomycetemcomitans leukotoxin

    DEFF Research Database (Denmark)

    Reinholdt, Jesper; Poulsen, Knud; Kilian, Mogens;

    associated with a highly aggressive form of disease in adolescents of African descent (1). An earlier report (2) identified the β2 integrin LFA-1 (αLβ2) as a cell surface receptor for LtxA. Whether the LtxA-reactive site involves αL (CD11a), β2 (CD18), or both of these subunits, is unknown.  Notably...

  14. Complete Genome Sequence of Paenibacillus strain Y4.12MC10, a Novel Paenibacillus lautus strain Isolated from Obsidian Hot Spring in Yellowstone National Park

    Energy Technology Data Exchange (ETDEWEB)

    Mead, David [University of Wisconsin, Madison; Lucas, Susan [U.S. Department of Energy, Joint Genome Institute; Copeland, A [U.S. Department of Energy, Joint Genome Institute; Lapidus, Alla L. [U.S. Department of Energy, Joint Genome Institute; Cheng, Jan-Fang [U.S. Department of Energy, Joint Genome Institute; Bruce, David [Los Alamos National Laboratory (LANL); Goodwin, Lynne A. [Los Alamos National Laboratory (LANL); Pitluck, Sam [U.S. Department of Energy, Joint Genome Institute; Chertkov, Olga [Los Alamos National Laboratory (LANL); Zhang, Xiaojing [Los Alamos National Laboratory (LANL); Detter, J. Chris [U.S. Department of Energy, Joint Genome Institute; Han, Cliff [Los Alamos National Laboratory (LANL); Tapia, Roxanne [Los Alamos National Laboratory (LANL); Land, Miriam L [ORNL; Hauser, Loren John [ORNL; Chang, Yun-Juan [ORNL; Kyrpides, Nikos C [U.S. Department of Energy, Joint Genome Institute; Ivanova, N [U.S. Department of Energy, Joint Genome Institute; Ovchinnikova, Galina [U.S. Department of Energy, Joint Genome Institute; Woyke, Tanja [U.S. Department of Energy, Joint Genome Institute; Brumm, Catherine [United States Department of Energy Joint Genome Institute; Hochstein, Rebecca [Lucigen Corporation, Middleton, Wisconsin; Schoenfeld, Thomas [Lucigen Corporation, Middleton, Wisconsin; Brumm, Phillip [University of Wisconsin, Madison

    2012-01-01

    Paenibacillus speciesY412MC10 was one of a number of organisms initially isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA. The isolate Y412MC10 was initially classified as a Geobacillus sp. based on its isolation conditions and similarity to other organisms isolated from hot springs at Yellowstone National Park. Comparison of 16 S rRNA sequences within the Bacillales indicated that Geobacillus sp.Y412MC10 clustered with Paenibacillus species and not Geobacillus; the 16S rRNA analysis indicated the organism was a strain of Paenibacillus lautus. Lucigen Corp. prepared genomic DNA and the genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute. The genome of Paenibacillus lautus strain Y412MC10 consists of one circular chromosome of 7,121,665 bp with an average G+C content of 51.2%. The Paenibacillus sp.Y412MC10 genome sequence was deposited at the NCBI in October 2009 (NC{_}013406). Comparison to other Paenibacillus species shows the organism lacks nitrogen fixation, antibiotic production and social interaction genes reported in other Paenibacilli. Over 25% of the proteins predicted by the Y412MC10 genome share no identity with the closest sequenced Paenibacillus species; most of these are predicted hypothetical proteins and their specific function in the environment is unknown.

  15. How can carbon favor planar multi-coordination in boron-based clusters? Global structures of CB(x)E(y)(2-) (E = Al, Ga, x + y = 4).

    Science.gov (United States)

    Cui, Zhong-hua; Sui, Jing-jing; Ding, Yi-hong

    2015-12-21

    With the high preference in forming multi-center bonding, boron has been a miracle ligand in constructing diverse planar multi-coordinate (pM) (tetra/hyper) species. Unfortunately, the boron ligand usually dislikes encompassing a pM carbon (pMC) due to the high competition with pM boron (pMB), which makes the realization of boron-based pMC very difficult and quite challenging. Herein, we propose a strategy that by means of cooperative doping and charge-compensation, we can successfully improve and tune the stability of pMC relative to pMB for CB4(2-). In the free CBxEy(2-) (E = Al/Ga) species, ptC is thermodynamically less stable than the global ptB in mono- and di-substituted systems, in agreement with the results of Boldyrev and Wang. However, the thermodynamic preference of pMC increases along with the Al/Ga-doping. The pMC species can be further stabilized by the introduction of the alkaline-earth counterion (Mg(2+)). CB2E2Mg (E = Al, Ga) designed in the present study represents the first successful design of a boron-based planar penta-coordinate carbon (ppC) structures as the global minima. The strategy proposed in this study should be useful in the manipulation of competition between exotic pMC and pMB in B-based systems. PMID:26574884

  16. Evolutionary generation of high order Runge - Kutta - Nyström type pairs for solving y(4) = f (x,y)

    Science.gov (United States)

    Famelis, I. Th.; Tsitmidelis, S.; Tsitouras, Ch.

    2016-06-01

    We present a new Runge - Kutta - Nyström type pair of orders 8(6) for the solution of a special fourth order initial value problem. To achieve this, a set of non - linear equations is solved using differential evolution technique.

  17. A Measurement of the Pseudoscalar DecayConstant fDs using Charm-Tagged Events in e+e- Collisions at the Y(4S)

    Energy Technology Data Exchange (ETDEWEB)

    Stelzer, Jorg; /Stanford U., Phys. Dept. /SLAC

    2006-10-10

    The decay constant f{sub D{sub s}} of the pseudoscalar strange charm meson D{sub s}{sup +} is an important benchmark test of the theoretical methods that quantitatively describe the nonperturbative low-energy regime of QCD, the theory of the strong interaction. A confirmation of the validity of these predictive methods, foremost lattice QCD, in the sector of heavy-light meson decay constants increases trust in the calculation of f{sub B}, which is an important number for the measurement of the CKM matrix element V{sub td} in B{sup 0}{bar B}{sup 0}-mixing events. From October 1999 through July 2004, the BABAR experiment, located at the PEP-II storage ring at the Stanford Linear Accelerator Center, collected 230.2 fb{sup -1} of data in e{sup +}e{sup -} collision at {radical}s = 10.58 GeV. In this thesis, these data are searched for e{sup +}e{sup -} {yields} c{bar c} events by identifying sets of charged and neutral pions and charged kaons, consistent with the decay of a charm meson, D{sup 0}, D{sup +}, D{sub s}{sup +}, or D*{sup +}. A sample of 510,000 charmed mesons with a momentum consistent with e{sup +}e{sup -} {yields} c{bar c} events is identified.

  18. Activation of a P2Y4-like purinoceptor triggers an increase in cytosolic [Ca2+] in the red blood cells of the lizard Ameiva ameiva (Squamata, Teiidae)

    OpenAIRE

    Sartorello R.; Garcia C.R.S.

    2005-01-01

    An increasing number of pathophysiological roles for purinoceptors are emerging, some of which have therapeutic potential. Erythrocytes are an important source of purines, which can be released under physiological and physiopathological conditions, acting on purinergic receptors associated with the same cell or with neighboring cells. Few studies have been conducted on lizards, and have been limited to ATP agonist itself. We have previously shown that the red blood cells (RBCs) of the lizard ...

  19. Association of the α2δ1 Subunit with Cav3.2 Enhances Membrane Expression and Regulates Mechanically Induced ATP Release in MLO-Y4 Osteocytes

    OpenAIRE

    Thompson, William R.; Majid, Amber S.; Czymmek, Kirk J; Ruff, Albert L.; García, Jesús; Duncan, Randall L.; Farach-Carson, Mary C.

    2011-01-01

    Voltage sensitive calcium channels (VSCCs) mediate signaling events in bone cells in response to mechanical loading. Osteoblasts predominantly express L-type VSCCs composed of the α1 pore-forming subunit and several auxiliary subunits. Osteocytes, in contrast, express T-type VSCCs, but a relatively small amount of L-type α1 subunits. Auxiliary VSCC subunits have several functions including modulating gating kinetics, trafficking of the channel and phosphorylation events. The influence of the ...

  20. Co-Cr-Mo Alloy Particles Induce Tumor Necrosis Factor Alpha Production in MLO-Y4 Osteocytes: A Role for Osteocytes in Particle Induced Inflammation

    OpenAIRE

    Kanaji, Arihiko; Caicedo, Marco S.; Virdi, Amarjit S.; Sumner, D. Rick; Hallab, Nadim J.; Sena, Kotaro

    2009-01-01

    Wear debris-induced osteolysis is purportedly the limiting problem affecting the long term results of joint arthroplasty. Pathogenic effects of wear debris in peri-implant cells such as macrophages, osteoblasts and osteoclasts have been well studied. In contrast, the affects of wear-debris on osteocytes, which make up over 90% of all bone cells, remains unknown. We hypothesized that metal implant debris can induce the proinflammatory response in osteocytes. This study demonstrated the effects...

  1. Gene expression signatures of a fibroblastoid preosteoblast and cuboidal osteoblast cell model compared to the MLO-Y4 osteocyte cell model☆

    OpenAIRE

    Yang, Wuchen; Harris, Marie A; Heinrich, Jelica Gluhak; Guo, Dayong; Bonewald, Lynda F.; Harris, Stephen E.

    2008-01-01

    In the osteoblast 2T3 cell model, 326 genes significantly increase in expression as subconfluent fibroblastic 2T3 cells become confluent and cuboidal. This gene set includes BMP2/4, Dlx2/5, Runx2, Osterix and Lrp5, as well as TGFβ regulated genes. Both activated or total nuclear Smad158 and Smad2 levels increase as they become confluent, and β-catenin protein expression increases as 2T3 cells become confluent, reflecting a set of genes involved in early preosteoblast to osteoblast commitment,...

  2. Polycystin 2 is involved in the nitric oxide production in responding to oscillating fluid shear in MLO-Y4 cells.

    Science.gov (United States)

    Xu, Huiyun; Guan, Ying; Wu, Jiawei; Zhang, Jian; Duan, Jin; An, Long; Shang, Peng

    2014-01-22

    As a mechano-calcium channel, polycystin2 (PC2) play an important role in the response of renal epithelial cells to fluid flow shear stress. In bone tissue, osteocytes are well known as the main mechanosensory cells, and sensitive to fluid flow stimulus in vitro. In the study, we investigated the effects of oscillating fluid flow (OFF, 2 h, 1 Hz, 1.0 Pa) on the release of Nitric Oxide (NO) and ProstaglandinE2 (PGE2), and the role of PC2 on the release. Our findings demonstrate that PC2 expression increases after 2 h of OFF, and silencing PC2 by RNAi inhibits downstream NO production and iNOS expression, but does not affect the response of PGE2 to OFF. PMID:24268313

  3. Clinical efficacy of subgingivally delivered 0.5% controlled release azithromycin gel in the management of chronic periodontitis

    Directory of Open Access Journals (Sweden)

    Prashant Tyagi

    2011-01-01

    Full Text Available Background: Recent developments suggest that the local delivery of antimicrobials into periodontal pockets can improve periodontal health. Azithromycin (AZM has a wide antimicrobial spectrum of action toward anaerobic bacteria as well as Gram-negative bacilli. It is effective against periodontal pathogens such as Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Therefore, this study was undertaken to investigate the clinical effectiveness of AZM at 0.5% concentration in an indigenously prepared bioabsorbable controlled release gel as an adjunct to non-surgical mechanical therapy in the treatment of chronic periodontitis. Materials and Methods: Thirty sites in patients with chronic periodontitis and probing depth (PD 4-6 mm were categorized randomly into two treatment groups: Scaling and root planing (SRP plus 0.5% AZM gel (group 1 and SRP only (group 2. Clinical evaluation was undertaken using the Gingival Index (GI of Loe and Silness and plaque was assessed using the Turesky et al. modification of Quigley Hein Index at baseline and 21 days. Pocket PD and clinical attachment level (CAL were also measured. Statistical Analysis: Results were expressed as mean ± standard deviation and percentages and the data were analyzed using Statistical Package for Social Sciences (SPSS version 16.0, SPSS, Chicago, IL software. Result: Both therapies resulted in significant improvements. Mean reduction in GI from baseline to 21 days was 1.20 ± 0.41 and 0.73 ± 0.45 in group 1 and group 2, respectively. Plaque Index also improved through the study period in both groups, i.e., 0.86 ± 0.51 in group 1 and 1.6 ± 0.97 in group 2. Mean PD reduced significantly with SRP plus AZM gel application in group 1, i.e., 2.1 ± 0.91 mm as compared to 1.0 ± 1.06 mm achieved with SRP alone. A significant gain in mean CAL gain was observed in the test group (1.8 ± 0.63 mm as compared to control group (1.0 ± 1.06 mm. Conclusion: Although both treatment

  4. Scaling and root planning and locally delivered minocycline could reduce the load of Prevotella intermedia in an interdependent pattern, which correlated with symptomatic improvements of chronic periodontitis: a short-term randomized clinical trial

    Directory of Open Access Journals (Sweden)

    Deng S

    2015-12-01

    Full Text Available Shuli Deng,1 Ying Wang,1 Wei Sun,1 Hui Chen,1 Gang Wu2 1Department of Conservative Dentistry, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, Hangzhou, People’s Republic of China; 2Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam (ACTA, VU University Amsterdam and University of Amsterdam, MOVE Research Institute Amsterdam, Amsterdam, the Netherlands Background: To evaluate the respective or combinatory efficacy of locally delivered 2% minocycline (MO and scaling and root planning (SRP by assessing both clinical parameters and the loads of four main periodontal pathogens in treating chronic periodontitis (CP.Methods: Seventy adults with CP were randomly assigned to the three treatment groups: 1 SRP alone; 2 MO alone; and 3 combinatory use of SRP and MO (SRP + MO. Before and 7 days after the treatments, we evaluated both clinical parameters (pocket depth [PD] and sulcus bleeding index [SBI] and the gene load of four main periodontal pathogens (Aggregatibacter actinomycetemcomitans [Aa], Fusobacterium nucleatum [Fn], Porphyromonas gingivalis [Pg], and Prevotella intermedia [Pi].Results: The bacterial prevalence per patient was Aa, 31.25%; Fn, 100%; Pg, 95.31%; and Pi, 98.44%. Seven days after treatments, the three treatments could significantly reduce both PD and SBI, but not detection frequencies of the four pathogens. For PD, the reduction efficacy of SRP + MO was significantly higher than that of both MO and SRP. Only Pg responded significantly to SRP. Pg and Fn could be significantly reduced in the presence of MO. Only SRP + MO but not the respective showed a significant reduction effect on the gene load of Pi. The reduction of PD significantly correlated with the gene load of Pi (r=0.26; P=0.042 but not of the other bacteria.Conclusion: SRP and MO could reduce the load of Pi in an interdependent pattern, which correlated with symptomatic improvements of CP

  5. Detection of bacterial species involved in perimplantitis concerned with cultural and RT-PCR

    Directory of Open Access Journals (Sweden)

    Marcello Gatti

    2010-06-01

    Full Text Available Dental implants offer new treatment options for edentulous either partially or completely, now represent a viable alternative to conventional fixed protheses. Dental implants are colonized by a flora dominated by Gram-positive facultative aerobic, while in patients with bone loss and formation of pockets peri-implant diseases was found a significant difference in the composition of microflora, bacteria, Gram-negative anaerobes in particular Fusobacterium spp., Treponema denticola (Spirochetes, Tannerella forsythensis, Aggregatibacter actinomycetemcomitans, Prevotella intermedia as interim black-pigmented bacteria, Porphyromonas gingivalis, often in high concentrations. Aims. The purpose of this study was to identify those at risk of perimplantitis using 2 techniques: RT-PCR examination of trade and culture. The results were compared taking into consideration the advantages and disadvantages of both methods. Materials and methods.We studied 24 patients (14 women and 10 men, aged, women between 43 and 76 years, with an average of 63.8 + / - 10.9 years, men between 45 and 88 years with a average of 64.3 years + / - 12.5 years. Was performed a double levy of sub-gingival plaque at multiple sites that had an implant CAL (clinical attachment level> 4mm in order to assess the microbiological identification with the two techniques: Examining culture and Real-Time PCR of Commerce ( Gum-Sunstar that identifies 4 bacterial species: A. actinomycetemcomitans (A.a., P.gingivalis (P.g., T.forsythensis (T.f., and T.denticola (T.d.. Results. All patients studied were positive to both tests with charger high: the consideration of tenure, with CFU / ml > 105, was positive in 66.6% of samples by:T.f., and P.g., in 12.5% for A.a., while T.d. not been sought by examining culture, the RT-PCR was positive, with high loads, in 95.8% of samples for T.f., in 79.1% for P.g., in 12.5% for A.a. and 20.8% for T.d.The test crop showed the presence of even P.intermedia in 91

  6. 牙周炎患者唾液中伴放线放线杆菌的检出状况分析%Prevalence of Actinobacillus actinomycetemcomitans in saliva of different types of periodontitis

    Institute of Scientific and Technical Information of China (English)

    冯向辉; 张立; 孟焕新; 徐莉; 陈智滨; 释栋

    2008-01-01

    Objective To investigate the prevalence of Actinobacillus actinomyeetemcomitans (Aa) in whole saliva of different types of periodontitis and compare the detections of Aa between saliva and pooled subgingival plaque sample, and analyze the relationship between Aa and clinical conditions. Methods Unstimulated whole saliva samples and pooled subgingival samples were collected from 50 aggressive periodontitis (AgP) patients, 48 chronic periodontitis (CP) patients and 25 subjects with no periodontitis, and Aa was detected in these samples by PCR method. Results The prevalence of Aa in whole saliva of AgP patients was significantly higher than in subjects with no periodontitis (32% vs. 4%, P <0. 01) and CP patients (32% vs. 15%, P <0. 05). Aa was also more frequently detected in whole saliva sample than in pooled subgingival sample of AgP patients (32% vs. 16%, P < 0.05). Subjects younger than 30 year sold were more likely to present Aa in whole saliva (OR = 3.23, P < 0. 05) and percentage of sites with bleeding index(BI) ≥3 over 70% was a risk indicator for the presence of Aa in whole saliva. Conclusions The detection of Aa in whole saliva sample of AgP patients was more frequent than in pooled subgingival plaque samples, and also more frequent than in CP patients and subjects with no periodontitis, which suggest that Aa may participate in the initiation and progression of aggressive periodontitis.%目的 检测不同类型牙周炎患者唾液中的伴放线放线杆菌(Actinobacillusactinomycetemcomitans,Aa),探讨唾液和集合龈下菌斑中Aa检出率的差异以及唾液中Aa的存在状况与牙周临床指标的关系. 方法 收集50例侵袭性牙周炎(aggressive periodontitis,AgP)患者、48例慢性牙周炎(chronic periedontitis,CP)患者和25例非牙周炎者的非刺激性全唾液和集合龈下菌斑,应用聚合酶链反应(PcR)技术检测两种样本中的Aa. 结果 Aa在AgP患者唾液中的检出率(32%)显著高于非牙周炎者(4%)和CP患者(15%),差异均有统计学意义(P<0.01,P<0.05),同时Aa在AgP患者唾液中的检出率也显著高于集合龈下菌斑样本(16%),差异亦有统计学意义(P<0.05).年龄≤30岁是唾液中存在Aa的危险指征(OR=3.23,P<0.05);出血指数≥3的位点超过70%与唾液中存在Aa有关(OR=19.21,P<0.01). 结论 AgP患者唾液样本中Aa的检出率明显高于集合龈下菌斑样本,亦高于CP患者和非牙周炎者,提示Aa可能参与AgP的发生和发展.

  7. 牙周基础治疗对侵袭性牙周炎患者唾液和龈沟液中牙周致病菌的作用%Effect of non-surgical periodontal therapy on putative periodontal pathogens in saliva and gingival crevicular fluids in patients with aggressive periodontitis

    Institute of Scientific and Technical Information of China (English)

    路瑞芳; 冯向辉; 徐莉; 孟焕新

    2012-01-01

    Objective To analyze the effect of non-surgical periodontal treatment on the prevalence of putative periodontal pathogens in saliva and gingival crevicular fluids (GCF) in patients with aggressive periodontitis(AgP).Methods Sixteen patients with AgP received non-surgical periodontal treatment,and un-stimulated saliva and GCF were collected before treatment and 2 weeks,2 months,4 months,6 months post-treatment.Periodontal pathogens including Aggregatibacter actinomycetemcomitans (Aa),Porphyromonas gingivalis (Pg),Tannerella forsythia (Tf),Treponema denticola (Td),Prevotella intermedia (Pi) and Fusobacterium nucleatum (Fn) were detected by polymerase chain reaction.Results The Aa,Pg,Tf,Td,Pi and Fn in saliva were detected in 7,16,13,14,9 and 14 patients respectively at baseline,and in 3,9,5,4,4 and 7 patients 2 weeks post-treatment.The prevalence of the 6 periodontal pathogens showed a tendency to increase at 2 months post-treatment,and was almost the same as that of baseline at 6 months post-treatment.While in GCF the prevalence of Aa,Pg,Tf,Td,Pi and Fn were 17% (11/64),95% (61/64),84% (54/64),72% (46/64),41% (26/64) and 56% (36/64) at baseline,and reduced to 8% (5/64),75% (48/64),28% (18/64),14% (9/64),16% (10/64) and 23% (15/64) at 2 weeks post-treatment (P < 0.05).The prevalence of the 6 periodontal pathogens remained a relatively low level [13% (8/64),73% (47/64),42% (27/64),23% (15/64),8% (5/64) and 38% (24/64)respectively] at 6 months post-treatment in GCF(P < 0.05) compared with baseline except Aa.There was difference of periodontal pathogens detection between saliva and GCF.Conclusions Non-surgical periodontal treatment can reduce pocket depth as well as periodontal pathogens in saliva and GCF.Periodontal ruaintenance every 2 months can keep the periodontal pathogens in GCF at a relatively low level.The periodontal pathogen detection in saliva can not completely replace the site detection.%目的

  8. Serum IgG antibody levels to periodontal microbiota are associated with incident Alzheimer disease.

    Directory of Open Access Journals (Sweden)

    James M Noble

    Full Text Available Periodontitis and Alzheimer disease (AD are associated with systemic inflammation. This research studied serum IgG to periodontal microbiota as possible predictors of incident AD.Using a case-cohort study design, 219 subjects (110 incident AD cases and 109 controls without incident cognitive impairment at last follow-up, matched on race-ethnicity, were drawn from the Washington Heights-Inwood Columbia Aging Project (WHICAP, a cohort of longitudinally followed northern Manhattan residents aged >65 years. Mean follow-up was five years (SD 2.6. In baseline sera, serum IgG levels were determined for bacteria known to be positively or negatively associated with periodontitis (Porphyromonas gingivalis, Tannerella forsythia, Actinobacillus actinomycetemcomitans Y4, Treponema denticola, Campylobacter rectus, Eubacterium nodatum, and Actinomyces naeslundii genospecies-2. In all analyses, we used antibody threshold levels shown to correlate with presence of moderate-severe periodontitis.Mean age was 72 years (SD 6.9 for controls, and 79 years (SD 4.6 for cases (p640 ng/ml, present in 10% of subjects was associated with increased risk of AD (HR = 2.0, 95%CI: 1.1-3.8. This association was stronger after adjusting for other significant titers (HR = 3.1, 95%CI: 1.5-6.4. In this model, high anti-E. nodatum IgG (>1755 ng/ml; 19% of subjects was associated with lower risk of AD (HR = 0.5, 95%CI: 0.2-0.9.Serum IgG levels to common periodontal microbiota are associated with risk for developing incident AD.

  9. Effect of the modification of a natural mexican zeolite in the sorption of cadmium and 4-chloro phenol; Efecto de la modificacion de una zeolita natural mexicana en la sorcion de cadmio y 4-clorofenol

    Energy Technology Data Exchange (ETDEWEB)

    Cortes M, R. [ININ, 52750 La Marquesa, Estado de Mexico (Mexico)

    2007-07-01

    Clinoptilolite type zeolite is a material of relative abundance in Mexico, which possess ion exchange properties and it can be used in the removal of metal ions from polluted waters. The external surface of zeolites can be modified with cationic surfactants. This modification could have a negative effect on the removal of metal ions and provides to the material the capacity to adsorb phenolic compounds. For this reason, it is important to know the capability of the modified material on the sorption of metal ions and phenolic compounds, simultaneously. The aim of this work was to evaluate the effect of the external surface modification with surfactant of a Mexican zeolite over its sorption capacity of cadmium and 4-chloro phenol, in batch and column systems. To accomplish that, a clinoptilolite type zeolitic rock from a deposit located in the state of Sonora, Mexico, was used. It was ground, sieved and characterized with different techniques; and its external surface area was modified with hexadecyltrimethylammonium bromide (HDTMA-Br). A grain size fraction was selected to carry out sorption kinetics and equilibrium experiments, as well as packed column tests with zeolitic material and solutions of cadmium and 4-chloro phenol. There are different models proposed in literature that are used to describe sorption kinetics and equilibrium. In this work, the sorption experimental results were adjusted to some of these models to identify controlling mechanisms on the kinetics and equilibrium of the studied systems. The results showed that the cadmium adsorption on natural and modified zeolite was similar in batch systems. For the case of 4-chloro phenol sorption, it was observed that natural zeolite does not retain this compound, while in modified zeolite the sorption is better than other comparable materials. The results also showed that for the case of cadmium sorption, the mechanism involved was ion exchange; while for sorption of 4-chloro phenol, a partition mechanism was implicated. Basic design parameters for the sorption of cadmium on natural and modified zeolites were calculated from breakthrough curves obtained from column experiments, by using simplified models. Column adsorption capacities for these two systems were good compared with other adsorbents and column efficiencies achieved were acceptable according to maximum adsorption capacities obtained for batch systems. For 4-chloro phenol, the same models were applied to calculate design parameters and column adsorption capacity and reasonable results were obtained. In this work was demonstrated that the field for application of natural zeolites as adsorbents can be amplified for the removal or organic pollutants from wastewaters, by the modification of their external surfaces with cationic surfactants, without having a significant effect on their ion exchange properties used for the uptake of heavy metals from polluted waters. (Author)

  10. Análisis de libros de texto de 3º y 4º de ESO para la mejora de la enseñanza de la Física

    OpenAIRE

    Cucharero-López, María del Carmen

    2013-01-01

    Actualmente, se podría pensar que en un futuro próximo el libro de texto acabará siendo reemplazado por las Tecnologías de la Información y de la Comunicación (TIC) y que carece de sentido dedicarle tiempo. Sin embargo, no hay aún evidencias claras para tal afirmación. De hecho, según algunos autores, la evolución de los materiales didácticos todavía no ha llegado a reemplazar al material en formato papel, aunque sea cada vez más frecuente el uso de soportes digitales. Además, ...

  11. EL RECURSO DIDÁCTICO DEL “JUEGO EXPERIMENTAL” DESARROLLADO MEDIANTE EL TRABAJO COOPERATIVO EN LAS ASIGNATURAS DE BIOLOGÍA Y GEOLOGÍA EN 3º Y 4º ESO.

    OpenAIRE

    Pérez-Esteban, Rosa Helena

    2015-01-01

    El juego está presente en el ser humano como motor de aprendizaje desde las primeras etapas del desarrollo cognitivo y desde los inicios de la humanidad como recurso educativo (Vopel, 2000). Por otra parte se ha demostrado ampliamente la importancia del empleo del trabajo cooperativo para obtener aprendizajes significativos (Slavin, 1983) y respecto a otros modelos de trabajo se ha evidenciado que los esfuerzos en los grupos cooperativos obtienen un mejor rendimiento que en los grupos de esfu...

  12. Antiproton-proton elastic scattering at 3.0 and 4.0 GeV/C; Difusion elastica antiproton-proton a 3,0 y 4,0 GeV/C

    Energy Technology Data Exchange (ETDEWEB)

    Unamuno, S.

    1965-07-01

    This paper presents the results-obtained in studying the two-prong interactions observed in the Saclay 81 cm hydrogen bubble chamber exposed to the 3.0 and 4.0 GeV/c antiproton beams from CERN Proton-Synchroton. Total elastic cross-sections corresponding to both energies are given. The results are given. The results are compared with those of p-p scaterring at different energies and with those of p-p scattering. Several optical-models, from the simples one (the black disk model) to a rather elaborated, four-parameters model have been applied. These models can explain some of the experimental results but fail in predicting the angular distribution of large angle scattering. (Author)

  13. Analysis of the 3 and 4 cycles with extensions in the operation of the CNLV U-1; Analisis de los ciclos 3 y 4 con extensiones en la operacion de la CNLV U-1

    Energy Technology Data Exchange (ETDEWEB)

    Montes T, J.L.; Torres A, C.; Perusquia C, R. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)

    1992-08-15

    The objective of the report is the comparison of the radial distributions of burned in the core among the results of the simulation of the Laguna Verde Central U-1 reactor during the operation of the cycles 1 to 4 and the data of the operation with information provided by the fuel supplier. (Author)

  14. Percepción del alumnado sobre la evaluación en Geografía e Historia en 3º y 4º de Educacion Secundaria Obligatoria (ESO)

    OpenAIRE

    Trigueros Cano, Francisco Javier

    2016-01-01

    La información sobre las prácticas evaluadoras y sobre los procedimientos, criterios e instrumentos de evaluación y calificación, desde el punto de vista del alumnado, supone un material de gran valor para entender mejor la realidad de nuestras aulas y comprender los porqués de los resultados académicos en ciencias sociales, así como el grado de corresponsabilidad en los mismos por parte del alumnado. En este sentido, al recoger las apreciaciones de los estudiantes sobre la evaluación de los ...

  15. 正畸治疗中牙龈增生的致病因素分析%Analysis of the pathogenesis of gingival enlargement in orthodontic treatment

    Institute of Scientific and Technical Information of China (English)

    陆静; 龚逸明; 丁小军

    2010-01-01

    目的 探讨正畸治疗中牙龈增生的相关致病因素.方法 12例因接受固定正畸治疗而出现牙龈增生的患者纳入牙龈增生组,对照组为12例牙龈健康者.分别于基线时采集两组研究对象的牙周检查指标、龈下菌斑标本和龈沟液标本,采用实时PCR技术对龈下菌斑内的牙龈卟啉单孢菌(Pg)、伴放线放线杆菌(Aa)、中间普氏菌(Pi)、齿密螺旋体(Td)和福赛氏类杆菌(Tf)进行定量检测,使用ELISA法测量龈沟液中白介素-1β的含量,比较牙龈增生组和对照组各项指标间的差异.对牙龈增生组患者实施牙周基础治疗,并于4周后重新采样比较牙周治疗前后上述指标间的差异.结果 基线时,牙龈增生组中龈下菌斑内五种牙周可疑微生物的检出率均显著高于对照组(P<0.05),龈沟液内白介素-1β的含量显著高于对照组(P<0.05).牙周基础治疗后4周时,牙龈增生患者的各项牙周检查指标均明显下降,同时Pg、Aa和Td的检出率、细菌数量以及龈沟液内白介素-1β的含量均显著减少(P<0.05).结论 Pg、Aa和Td等牙周病原菌和白介素-1β与正畸治疗中牙龈增生的发生和发展密切相关.%Objective To investigate the pathogenesis of gingival enlargement (GE) in orthodontic treatment. Methods The study sample included 12 patients with GE undergoing fixed orthodontic treatment and 12 periodontally healthy controls. At baseline, periodontal variables,subgingival plaque samples and gingival cervical fluid (GCF) samples were taken from two pre-selected sites in both the GE group and control group. The levels of Porphyromonas gingivalis (Pg),Aggregatibacter actinomycetemcomitans (Aa), Prevotella intermedia (Pi), Treponema denticola (Td) and Tannerella forsythensis (Tf) were determined by real-time PCR. GCF interleukin (IL)-1β were detected by ELISA. Periodontal therapy was administered to the patients in the GE group and all the parameters were reassessed after 4

  16. Reduction of periodontal pathogens adhesion by antagonistic strains

    NARCIS (Netherlands)

    Van Hoogmoed, C. G.; Geertsema-Doornbusch, G. I.; Teughels, W.; Quirynen, M.; Busscher, H. J.; Van der Mei, H. C.

    2008-01-01

    Introduction: Periodontitis results from a shift in the subgingival micro. ora into a more pathogenic direction with Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans considered as periodontopathogens. In many cases, treatment procures only a temporary shift t

  17. Detection of pathogens from periodontal lesions

    Directory of Open Access Journals (Sweden)

    Malheiros Veruska de João

    2004-01-01

    Full Text Available OBJECTIVE: To comparatively detect A. actinomycetemcomitans and F. nucleatum from periodontal and healthy sites. METHODS: Subgingival clinical samples from 50 periodontitis adult patients and 50 healthy subjects were analyzed. Both organisms were isolated using a trypticase soy agar-bacitracin-vancomycin (TSBV medium and detected by PCR. Conventional biochemical tests were used for bacteria identification. RESULTS: A. actinomycetemcomitans and F. nucleatum were isolated in 18% and 20% of the patients, respectively, and in 2% and 24% of healthy subjects. Among A. actinomycetemcomitans isolates, biotype II was the most prevalent. Primer pair AA was 100% sensitive in the detection of A. actinomycetemcomitans from both subject groups. Primers ASH and FU were also 100% sensitive to detect this organism in healthy subject samples. Primer pair FN5047 was more sensitive to detect F. nucleatum in patients or in healthy samples than primer 5059S. Primers ASH and 5059S were more specific in the detection of A. actinomycetemcomitans and F. nucleatum, respectively, in patients and in healthy subject samples. CONCLUSIONS: PCR is an effective tool for detecting periodontal pathogens in subgingival samples, providing a faster and safer diagnostic tool of periodontal diseases. The method's sensitivity and specificity is conditioned by the choice of the set of primers used.

  18. Periodontal pathogens in erupting third molars of periodontally healthy subjects.

    Science.gov (United States)

    Rajasuo, A; Sihvonen, O J; Peltola, M; Meurman, J H

    2007-09-01

    The presence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis in bacteriologic samples of 5-7-mm deep mandibular third-molar pericoronal pockets was analysed by polymerase chain reaction, to test the hypothesis that these sites would harbour the bacteria. The patients were periodontally healthy 20-year-old Finnish male conscripts. Sixteen had acute pericoronitis, 28 chronic pericoronitis, and 15 were symptom-free controls. A. actinomycetemcomitans was detected in only 7% of the samples from chronic pericoronitis cases, whereas P. gingivalis was positive in 20% of the symptom-free versus 69% (P = 0.018) of the acute and 57% (P = 0.044) of the chronic cases. The percentages for P. intermedia were 93, 94 and 93%, and for T. forsythensis 47, 63 and 57%, respectively. These results confirm that, apart from A. actinomycetemcomitans, periodontopathogens are common in third-molar sites in periodontally healthy individuals.

  19. Association of serum immunoglobulin G (IgG) levels against two periodontal pathogens and prothrombotic state: a clinical pilot study

    NARCIS (Netherlands)

    S. Bizzarro; E.A. Nicu; U. van der Velden; M.L. Laine; B.G. Loos

    2010-01-01

    Objective: Periodontitis is associated with cardiovascular diseases (CVD). In our previous studies a prothrombotic state has been observed in periodontitis, which contributes to the risk of CVD. The aim of this study was to investigate whether serum IgG levels against Aggregatibacter actinomycetemco

  20. Planar vs. three-dimensional X6(2-), X2Y4(2-), and X3Y3(2-) (X, Y = B, Al, Ga) metal clusters: an analysis of their relative energies through the turn-upside-down approach.

    Science.gov (United States)

    El Bakouri, Ouissam; Solà, Miquel; Poater, Jordi

    2016-08-01

    Despite the fact that B and Al belong to the same group 13 elements, the B6(2-) cluster prefers the planar D2h geometry, whereas Al6(2-) favours the Oh structure. In this work, we analyse the origin of the relative stability of D2h and Oh forms in these clusters by means of energy decomposition analysis based on the turn-upside-down approach. Our results show that what causes the different trends observed is the orbital interaction term, which combined with the electrostatic component do (Al6(2-) and Ga6(2-)) or do not (B6(2-)) compensate the higher Pauli repulsion of the Oh form. Analysing the orbital interaction term in more detail, we find that the preference of B6(2-) for the planar D2h form has to be attributed to two particular molecular orbital interactions. Our results are in line with a dominant delocalisation force in Al clusters and the preference for more localised bonding in B metal clusters. For mixed clusters, we have found that those with more than two B atoms prefer the planar structure for the same reasons as for B6(2-). PMID:27020701

  1. Role of the Property Engineering in the units' construction of the Cernavoda nuclear power plant; Papel de la ingenieria de la propiedad en la construccion de las nuevas unidades 3 y 4 de la C.N. de Cernavoda (Rumania)

    Energy Technology Data Exchange (ETDEWEB)

    Esteban-Zazo Gomez, J. A.; Varas Suarez, M. I.; Dehalu, J. M.; Giura, A.

    2010-07-01

    The engineering of the property acts as owner's representative to ensure that the technical execution of the work is done according to the specifications. Normally, his paper complements the role of project management often tends to be more focused on the monitoring and supervision of commercial and financial aspects of it.

  2. Methylene blue and 4-chloro phenol degradation by photo catalysis with ultraviolet light, using TiO{sub 2} as catalyst; Degradacion de azul de metileno y 4-clorofenol por fotocatalisis con luz ultravioleta, utilizando TiO{sub 2} como catalizador

    Energy Technology Data Exchange (ETDEWEB)

    Martinez H, A.

    2010-07-01

    Within the decontamination and remediation processes of the contaminated waters, as the refining or tertiary processes are the Advanced Oxidation Technologies. Among this technology is the heterogeneous photo catalysis, which is the object of this work to de grate 4-chloro phenol and methylene blue, using as semiconductor commercial titanium dioxide (TiO{sub 2}). On the degradation the combination is exposed in the use of TiO{sub 2} under gamma irradiation of {sup 60}Co at different doses 400, 500, 800, 1000 and 1500 kGy. The organic compounds degradation was determined and the results show that to more radiation dose, the material is modified in such way that shows a major absorption of the organic compound, in the same way it is determined that to more dose which undergoes the TiO{sub 2} generally a major degradation is observed, but also it is has to give a more time of previous stabilization, for that the degradation is observed of better way. (Author)

  3. Bacterial endocarditis due to eikenella corrodens: A case report

    Directory of Open Access Journals (Sweden)

    Mahapatra A

    2003-01-01

    Full Text Available Of all the causes of bacterial endocarditis, HACEK group consisting of Haemophilus, Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella Kingae are rare causative agents. We report a case of bacterial endocarditis by E. corrodens, which is one of the members of the HACEK group.

  4. 非线性4n阶常微分方程的非线性三点边值问题解的存在性%Existence of solutions of nonlinear three-point boundary value problems for nonlinear 4nth-order differential equation

    Institute of Scientific and Technical Information of China (English)

    高永馨; 高有

    2002-01-01

    利用"上下解"的方法,讨论了非线性4n阶常微分方程y(4n)=f(t,y,y′,y″,…,y(4n-1))满足条件g2i(y(2i)(a),y(2i+1)(a))=0 i=0,1,…,2n-3g4n-4(y(4n-4)(a),y(4n-3)(a),y(4n-2)(a),y(4n-1) (a))=0g4n-3(y(b),y′(b),…,y(4n-6)(b))=0g4n-2(y(4n-5)(b),y(4n-4)(b))=0g4n-1(y(4n-3)(b),y(4n-2)(b))=0g2i+1(y(2i+1)(c),y(2i+2)(c))=0 i=0,1,…,2n-4g4n-5(y(4n-5)(c),y(4n-4)(c),…,y(4n-1)(c))=0 的非线性三点边值问题解的存在性.

  5. Prevalence and distribution of principal periodontal pathogens worldwide

    DEFF Research Database (Denmark)

    Rylev, Mette; Kilian, Mogens

    2008-01-01

    to particular ethnic groups. AIM: This review analyzes to what extent observed differences in periodontal disease prevalence among ethnically or geographically distinct populations may be explained by restricted host adaptation of clones of principal periodontal pathogens. RESULTS: Carriage rates of several...... putative periodontal pathogens and particular subsets of these species vary between ethnic groups. Few of these differences can, with the limited information available, be directly related to differences in periodontal disease prevalence. Asian populations are regularly colonized with Actinobacillus...... actinomycetemcomitans serotype c with questionable pathogenic potential. Conversely, the JP2 clone of A. actinomycetemcomitans has enhanced virulence and causes significantly higher prevalence of aggressive periodontitis in adolescents whose descent can be traced back to the Mediterranean and Western parts of Africa...

  6. Combined orthodontic and periodontic treatment in a child with Papillon Lefèvre syndrome

    OpenAIRE

    AlSarheed, Maha A.; Al-Sehaibany, Fares S.

    2015-01-01

    A 9-year-old girl with Papillon-Lefèvre syndrome (PLS) was treated orthodontically 24 months after the start of mechanical and antibiotic therapy in adjunct with periodontal treatment every 6 weeks. After achieving stable periodontal conditions, orthodontic treatment was commenced to correct the teeth position, facial profile, and maxillary protraction. Following the combination therapy and a failure to detect Actinobacillus actinomycetemcomitans from any site in the oral cavity, orthodontic ...

  7. Cardiobacterium hominis endocarditis: two cases and a review of the literature

    OpenAIRE

    Malani, A. N.; Aronoff, D. M.; Bradley, S. F.; Kauffman, C A

    2006-01-01

    Cardiobacterium hominis, a member of the HACEK group (Haemophilus parainfluenzae, Haemophilus aphrophilus, and Haemophilus paraphrophilus, Actinobacillus actinomycetemcomitans, C. hominis, Eikenella corrodens, and Kingella species), is a rare cause of endocarditis. There are 61 reported cases of C. hominis infective endocarditis in the English-language literature, 15 of which involved prosthetic valve endocarditis. There is one reported case of C. hominis after upper endoscopy and none report...

  8. Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique

    Directory of Open Access Journals (Sweden)

    Mitić Aleksandar

    2013-01-01

    Full Text Available Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%. In a relatively high percentage we detected P. intermedia (25%, A. actinomycetemcomitans (20%, T. denticola (17%, T. forsythensis (15% and P. gingivalis (10%. After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%, P. intermedia (0%, T. forsythensis (0% and P. gingivalis (0%. The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished

  9. Prevalence analysis of putative periodontal pathogens in patients with aggressive periodontitis and healthy elderly

    OpenAIRE

    Edesi-Neuss, Lilian

    2005-01-01

    Marginale Parodontitis, die multikausale Erkrankung des Parodonts ist eine Infektionskrankheit, modifiziert durch Wirtsfaktoren und äußere Einflüße. Die als pathogene Mischflora bezeichnete Kombination kommensaler Mikroorganismen spielt die primäre Rolle in der Ätiopathogenese der Parodontitis. In der Aufstellung des Studienziels wurden einzelne Bakterienarten (T. forsythensis, P. gingivalis, A. actinomycetemcomitans, C. rectus, F. nucleatum, Fusobacterium spp., P. intermedia, E. corrodens, V...

  10. Analysis of the presence of pathogens which predict the risk of disease at peri-implant sites through polymerase chain reaction (PCR) Análise por reação em cadeia da polimerase (PCR) da presença de patógenos preditores de risco em sítios periimplantares

    OpenAIRE

    Joely Ângela de Oliveira Leitão; José Luiz De Lorenzo; Mario Julio Avila-Campos; Wilson Roberto Sendyk

    2005-01-01

    The presence of DNA of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Prevotella intermedia in the peri-implant sulcus samples of 19 partially edentulous patients was analyzed by polymerase chain reaction (PCR) and related to the depth of the peri-implant sulcus, bleeding on probing, and probable risk of disease. Ten of those patients presented a history of periodontal disease and nine of those did not. The DNA amplification of these pathogens was observed in seven sample...

  11. Prevalence of Periodontal Pathogens in Dental Plaque of Children

    OpenAIRE

    Gafan, Gavin P.; Lucas, Victoria S.; Roberts, Graham J.; Petrie, Aviva; Wilson, Michael; David A. Spratt

    2004-01-01

    Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, and Tannerella forsythensis have been implicated as the main etiological agents of periodontal disease. The purpose of this work was to estimate the prevalence of these organisms in plaque from children without gingivitis (group 1; n = 65) and from those with gingivitis (group 2; n = 53). Extracted DNA from plaque was subjected to two rounds of PCR targeting the 16S rRNA gene using both universal primers and species-specific prim...

  12. Aspectos clínicos, radiográficos e microbianos de uma família com expressiva prevalência de doença periodontal = Clinical, radiographics and microbiological aspects of a family with expressive prevalence of periodontal disease

    Directory of Open Access Journals (Sweden)

    Querido, Silvia Maria Rodrigues

    2006-01-01

    Full Text Available A periodontite agressiva é caracterizada por uma rápida perda de inserção conjuntiva e destruição óssea, podendo ocorrer na forma localizada ou generalizada. Na periodontite agressiva localizada ocorre o comprometimento, principalmente, dos dentes incisivos e primeiros molares permanentes. É a forma de doença periodontal mais associada com Actinobacillus actinomycetemcomitans. De modo distinto, a forma generalizada da periodontite agressiva afeta vários dentes em um ou ambos os arcos dentários. O objetivo do presente estudo foi estabelecer através de exames clínicos, radiográficos e microbianos o diagnóstico de uma família com expressiva prevalência de doença periodontal. Foram incluídos 5 indivíduos da mesma família, os quais foram submetidos à exame clínico periodontal e exame radiográfico de todos os dentes. Para a análise microbiológica foram selecionados no mínimo dois dentes para cada indivíduo, e a presença de Actinobacillus actinomycetemcomitans foi determinada pelo método de cultura bacteriana e reação em cadeia da polimerase. Dos indivíduos examinados, dois receberam o diagnóstico de periodontite incipiente, dois de periodontite agressiva localizada e um de periodontite agressiva generalizada. O Actinobacillus actinomycetemcomitans de máxima leucotoxicidade foi detectado em todos os indivíduos. Com base nestes resultados observou- se que os indivíduos apresentavam a mesma característica microbiana e diferenças na expressão e severidade da doença periodontal, sugerindo que outros fatores poderiam estar presentes modificando a manifestação clínica da doença

  13. Association between selected oral pathogens and gastric precancerous lesions.

    Directory of Open Access Journals (Sweden)

    Christian R Salazar

    Full Text Available We examined whether colonization of selected oral pathogens is associated with gastric precancerous lesions in a cross-sectional study. A total of 119 participants were included, of which 37 were cases of chronic atrophic gastritis, intestinal metaplasia, or dysplasia. An oral examination was performed to measure periodontal indices. Plaque and saliva samples were tested with real-time quantitative PCR for DNA levels of pathogens related to periodontal disease (Porphyromonas gingivalis, Tannerella forsythensis, Treponema denticola, Actinobacillus actinomycetemcomitans and dental caries (Streptococcus mutans and S. sobrinus. There were no consistent associations between DNA levels of selected bacterial species and gastric precancerous lesions, although an elevated but non-significant odds ratio (OR for gastric precancerous lesions was observed in relation to increasing colonization of A. actinomycetemcomitans (OR = 1.36 for one standard deviation increase, 95% Confidence Interval = 0.87-2.12, P. gingivalis (OR = 1.12, 0.67-1.88 and T. denticola (OR = 1.34, 0.83-2.12 measured in plaque. To assess the influence of specific long-term infection, stratified analyses by levels of periodontal indices were conducted. A. actinomycetemcomitans was significantly associated with gastric precancerous lesions (OR = 2.51, 1.13-5.56 among those with ≥ median of percent tooth sites with PD ≥ 3 mm, compared with no association among those below the median (OR = 0.86, 0.43-1.72. A significantly stronger relationship was observed between the cumulative bacterial burden score of periodontal disease-related pathogens and gastric precancerous lesions among those with higher versus lower levels of periodontal disease indices (p-values for interactions: 0.03-0.06. Among individuals with periodontal disease, high levels of colonization of periodontal pathogens are associated with an increased risk of gastric precancerous lesions.

  14. PCR detection of four periodontopathogens from subgingival clinical samples Detecção por PCR de quatro periodontopatógenos de pacientes com doença periodontal e de indivíduos sadios

    Directory of Open Access Journals (Sweden)

    Mario Julio Avila-Campos

    2003-04-01

    Full Text Available In this study, A. actinomycetemcomitans, B. forsythus, P. gingivalis and F. nucleatum were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. Subgingival clinical samples were collected with sterilized paper points and transported in VMGA III. From all the diluted clinical samples (1:10, DNA was obtained by boiling, and after centrifugation the supernatant was used as template. Specific primers for each bacterial species were used in PCR. PCR amplification was sensitive to identify these organisms. PCR products from each species showed a single band and can be used to identify periodontal organisms from clinical specimens. PCR detection odds ratio values for A. actinomycetemcomitans and B. forsythus were significantly associated with disease showing a higher OR values for B. forsythus (2.97, 95% CI 1.88 - 4.70. These results suggest a strong association among the studied species and the periodontal lesion.Em nosso estudo quatro periodontopatógenos foram isolados e identificados de placas subgengivais de 50 pacientes com doença periodontal e de 50 indivíduos sadios. As placas subgengivais foram coletadas com pontas de papel e transportadas em VMGA III. Foram realizadas diluições seriadas das amostras clínicas (1:10, e os DNA foram obtidos por fervura. Iniciadores específicos para cada bactéria foram usados no PCR. As amplificações mostraram-se sensíveis na identificação de A. actinomycetemcomitans, B. forsythus, P. gingivalis e F. nucleatum. As reações de PCR produziram bandas específicas para cada espécie e podem ser usadas na identificação desses organismos periodontais diretamente das amostras clínicas. Os valores de odds ratio para a detecção de A. actinomycetemcomitans e B. forsythus foram significativamente associados com a doença periodontal mostrando altos valores de OR para B. forsythus (2,97, 95% CI 1,88 - 4,70. Esses resultados sugerem uma forte associação entre os

  15. Detection of putative periodontal pathogens in subgingival specimens of dogs Detecção de patógenos periodontais em amostras subgengivais de cães

    Directory of Open Access Journals (Sweden)

    Sheila Alexandra Belini Nishiyama

    2007-03-01

    Full Text Available In this study, the presence of putative periodontal organisms, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, Fusobacterium nucleatum,Dialister pneumosintes,Actinobacillus actinomycetemcomitans,Campylobacter rectus,Eikenella corrodens and Treponema denticola were examined from subgingival samples of 40 dogs of different breeds with (25 and without (15 periodontitis, by using the PCR method. The PCR products of each species showed specific amplicons. Of the 25 dogs with periodontitis, P. gingivalis was detected in 16 (64% samples, C. rectus in 9 (36%, A. actinomycetemcomitans in 6 (24%, P. intermedia in 5 (20%, T. forsythensis in 5 (20%, F. nucleatum in 4 (16% and E. corrodens in 3 (12%. T. denticola and D. pneumosintes were not detected in clinical samples from dogs with periodontitis. Moreover, P. gingivalis was detected only in one (6.66% crossbred dog without periodontitis. Our results show that these microorganisms are present in periodontal microbiota of dogs with periodontitits, and it is important to evaluate the role of these putative periodontal microorganisms play in the periodontitis in household pets particularly, dogs in ecologic and therapeutic terms, since these animals might acquire these periodontopahogens from their respective owners.Neste estudo, a presença de patógenos periodontais, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, Fusobacterium nucleatum, Dialister pneumosintes, Actinobacillus actinomycetemcomitans, Campylobacter rectus, Eikenella corrodens e Treponema denticola foi determinada por PCR, em amostras subgengivais de 40 cães com (25 e sem (15 doença periodontal. Os produtos amplificados pelo PCR para cada espécie bacteriana mostraram amplicons específicos. Dos 25 cães apresentando doença periodontal, P. gingivalis foi detectado em 16 amostras (64%, C. rectus em 9 (36%, A. actinomycetemcomitans em 6 (24%, P. intermedia em 5 (20%, T. forsythensis em 5 (20

  16. Antimicrobial activity of magnolol and honokiol against periodontopathic microorganisms.

    Science.gov (United States)

    Chang, B; Lee, Y; Ku, Y; Bae, K; Chung, C

    1998-05-01

    Magnolol (1) and honokiol (2), main compounds from the stem bark of Magnolia obovata Thunb., were evaluated for an antimicrobial activity against periodontopathic microorganisms, Porphyromonas gingivalis, Prevotella gingivalis, Actinobacillus actinomycetemcomitans, Capnocytophaga gingivalis, and Veillonella disper, and a cytotoxicity against human gingival fibroblasts and epithelial cells. Our results indicate that magnolol and honokiol, although less potent than chlorhexidine, show a significant antimicrobial activity against these microorganisms, and a relatively low cytotoxic effect on human gingival cells. Thus, it is suggested that magnolol and honokiol may have a potential therapeutic use as a safe oral antiseptic for the prevention and the treatment of periodontal disease. PMID:9619121

  17. Anti-microbial Activity of Tulsi {Ocimum Sanctum (Linn.)} Extract on a Periodontal Pathogen in Human Dental Plaque: An Invitro Study

    Science.gov (United States)

    Devaraj, C.G.; Agarwal, Payal

    2016-01-01

    Introduction Tulsi is a popular healing herb in Ayurvedic medicine. It is widely used in the treatment of several systemic diseases because of its anti-microbial property. However, studies documenting the effect of Tulsi on oral disease causing organisms are rare. Hence, an attempt was made to determine the effect of Tulsi on a periodontal microorganism in human dental plaque. Aim To determine if Ocimum sanctum (Linn.) has an anti-microbial activity (Minimum Inhibitory Concentration and zone of inhibition) against Actinobacillus actinomycetemcomitans in human dental plaque and to compare the antimicrobial activity of Ocimum sanctum(Linn.) extract with 0.2% chlorhexidine as the positive control and dimethyl sulfoxide as the negative control. Materials and Methods A lab based invitro experimental study design was adopted. Ethanolic extract of Ocimum sanctum (Linn.) was prepared by the cold extraction method. The extract was diluted with an inert solvent, dimethyl sulfoxide, to obtain ten different concentrations (1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%) of extract. Plaque sample was collected from 05 subjects diagnosed with periodontal disease. Isolation of Actinobacillus actinomycetemcomitans from plaque samples was done using Tryptic Soy Serum Bacitracin Vancomycin agar (TSBV) medium. Identification of Actinobacillus actinomycetemcomitans was done based on cultural, microscopic, biochemical characterization and multiple drug resistance patterns. Anti-microbial activity of Ocimum sanctum (Linn.) extract was tested by agar well-diffusion method against 0.2% chlorhexidine as a positive control and dimethyl sulfoxide as a negative control. The zone of inhibition was measured in millimeters using Vernier callipers. Results At the 6% w/v concentration of Ocimum sanctum (Linn.) extract, a zone of inhibition of 22 mm was obtained. This was the widest zone of inhibition observed among all the 10 different concentrations tested. The zone of inhibition for positive control

  18. In vitro antibacterial activity of ethanolic extract of Morus alba leaf against periodontal pathogens

    Directory of Open Access Journals (Sweden)

    Shilpa Gunjal

    2015-01-01

    Results: P. gingivalis was the most sensitive organism against the M. alba extract with an MIC value of 1.95 mg/ml; while T. forsythia and P. gingivalis both were most sensitive organisms against chlorhexidine gluconate with MIC values of 0.00781 mg/ml. Conclusion: M. alba possess good antibacterial activity against A. actinomycetemcomitans, P. gingivalis and T. forsythia and thus would be beneficial for the prevention and treatment of periodontal disease. However, chlorhexidine gluconate was found to be more effective when compared to M. alba.

  19. [Markers of periodontal diseases and sensitivity to taromentine in patients with aggressive periodontitis].

    Science.gov (United States)

    Iverieli, M V; Abashidze, N O; Gogishvili, Kh B

    2009-04-01

    The aim of the research was to study sensitivity of specific microorganisms from the periodontal pockets of patients with rapidly progressive periodontal disease to Taromentine. 95 patients aged 21 to 35 years (50 women (52,6+/-33,62) and 45 men (47,36+/-3,62)) with rapidly progressive form of periodontal desease were observed. Porphiromonas gingivalis was identifide in 83 out of 95 patients (87,36+/-2,06). Prevotella intermedia - in 31 patients (32,6+/-2,750); Actinobacillus actinomycetemcomitans - in 23 patients (24,2+/-2,050); Bacteroides forsythus - in 19 patients (20,0+/-2,360); Treponema denticola - in 16 patients (16,84+/-2,190); Candida - in 11 patients (11,57+/-1,80). The sensitivity of all cultures to Taromentine was investigated: 134 (77,9+/-1,89) out of 183 identified markers demonstrated sensitivity to Taromentine. Demostrated sensitivity to Taromentine: 64 (37,2+/-1,06) out of 83 identified cultures of Porphiromonas gingivalis, 24 (13,95+/-1,85) out of 31 identified cultures of Prevotela intermedia, 18 (10,47+/-1,05) out of 23 identified cultures of Actinobacillus actinomycetemcomitans, 15 (8,7+/-1,86) out of 19 identified cultures of Bacteroides forsythus, and 13 (7,84+/-1,09) out of 16 identified cultures of Treponema denticola. Totally 38 (22,1+/-1,59) out of 172 identified periodontal markers demonstrated resistence to Taromentine. The results of analysis showed that Taromentine could be recommended in complex treatment of periodontal diseases. PMID:19430039

  20. Characterization and antibacterial performance of ZrCN/amorphous carbon coatings deposited on titanium implants

    Energy Technology Data Exchange (ETDEWEB)

    Lai, Chih-Ho [School of Medicine, China Medical University, Taichung, 404 Taiwan (China); Chang, Yin-Yu, E-mail: yinyu@mail2000.com.tw [Department of Mechanical and Computer-Aided Engineering, National Formosa University, Yunlin, Taiwan (China); Huang, Heng-Li [School of Dentistry, China Medical University, Taichung, Taiwan (China); Kao, Ho-Yi [Department of Materials Science and Engineering, Mingdao University, Changhua, Taiwan (China)

    2011-12-30

    Titanium (Ti)-based materials have been used for dental/orthopedic implants due to their excellent biological compatibility, superior mechanical strength and high corrosion resistance. The osseointegration of Ti implants is related to their composition and surface treatment. Better biocompatibility and anti-bacterial performances of Ti implant are beneficial for the osseointegration and for avoiding the infection after implantation surgery. In this study, nanocomposite ZrCN/amorphous carbon (a-C) coatings with different carbon contents were deposited on a bio-grade pure Ti implant material. A cathodic-arc evaporation system with plasma enhanced duct equipment was used for the deposition of ZrCN/a-C coatings. Reactive gas (N{sub 2}) and C{sub 2}H{sub 2} activated by the zirconium plasma in the evaporation process were used to deposit the ZrCN/a-C coatings. To verify the susceptibility of implant surface to bacterial adhesion, Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans), one of the major pathogen frequently found in the dental implant-associated infections, was chosen for in vitro anti-bacterial analyses. In addition, the biocompatibility of human gingival fibroblast (HGF) cells on coatings was also evaluated by a cell proliferation assay. The results suggested that the ZrCN/a-C coatings with carbon content higher than 12.7 at.% can improve antibacterial performance with excellent HGF cell compatibility as well.

  1. Bacteria prevalence in a large Italian population sample: a clinical and microbiological study.

    Science.gov (United States)

    Checchi, L; Gatto, M R; Checchi, V; Carinci, F

    2016-01-01

    The present study detects those bacterial species which are more strongly related to bleeding on probing, suppuration and smoking in periodontal-affected patients. Nine hundred and fifty-one patients with periodontal diseases were admitted to the Department of Periodontology and Implantology, Dental School of Bologna University where they underwent microbiological tests for six periodontal pathogens (Actinomyces actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola, Fusobacterium nucleatum and Tannerella forsythia). Cluster analysis explored the variables that mostly influence both the presence and absolute\\relative bacterial load. Logistic regression and multivariate linear regression quantifies these relations. The probability of recovering bacteria belonging to the Red Complex is greater by 25-48% in presence of bleeding on probing. When probing depth is less than 3 mm the probability of presence of each bacterial species is inferior in comparison with depth >6 mm both for Red Complex (of 20-37%), the Orange complex (of 41-61%) and Actinomyces actinomycetemcomitans (46%). Total bacterial cell count increases with pocket depth above all for the Red Complex. As Treponema Denticola and Tannerella Forsytia presence is associated with bleeding on probing and Prevotella intermedia presence with suppuration and smoking. The examination of these three as indicators of periodontitis evolution is suggested. PMID:27469569

  2. STUDY ON ADHERENCE ABILITY OF PERIODONTAL PATHOGENS AND CARIOGENIC BACTERIA TO HYDROXYAPATITE DISKS

    Institute of Scientific and Technical Information of China (English)

    WANG Min-feng; LI De-yi; LI Zong-lin

    2006-01-01

    Objective To study the adherence activity of six representative periodontopathic and cariogenic bacteria to hydroxyapatite disks. Methods Six periodontopathic and cariogenic bacteria of P. gingivalis, A.actinomycetemcomitans, F. nucleatum, S. sanguis, A. viscosus and S. mutans were cultured in modified MD-300 chemostat according to total fifteen experimental groups of single-specie and each pair of periodontal pathogens and cariogenic bacteria, respectively. After 1h attached live bacteria on removable hydroxyapatite disks was analyzed by culture technologies to evaluate the adherence level. Results The adherence activity of periodontopathic and cariogenic bacteria to HA was in the following order: S. sanguis > A. viscosus > S. mutans > A. actinomycetemcomitans > F. nucleatum > P. gingivalis. The number of periodontopathic bacteria to HA was enhanced by S.sanguis and A. viscosus, respectively. When mix-cultivated with S. mutans, the colonization of P. gingivalis was reduced significantly( P<0.001 ). Periodontopathic bacteria had no effect on the adherence activity of S. mutans and A. viscosus, except S. sanguis. Conclusion It was showed that the adherence activity of periodontal pathogens was weaker than that of cariogenic bacteria and emphasized the importance of bacterial adherence in determining the level of bacterial colonization on tooth surfaces. It was suggested that periodontopathic bacteria can utilize initial colonizers to become those predominant bacteria in periodontal ecosystem, which maybe have close relation to the periodontopathic mechanism.

  3. Detection of putative periodontal pathogens in non-insulin-dependent diabetes mellitus and non-diabetes mellitus by polymerase chain reaction.

    Science.gov (United States)

    Yuan, K; Chang, C J; Hsu, P C; Sun, H S; Tseng, C C; Wang, J R

    2001-02-01

    It has been assumed that there is a relationship between periodontal diseases and diabetes mellitus, however the putative periodontal microorganisms in non-diabetes mellitus (non-DM) individuals and non-insulin-dependent diabetes mellitus (NIDDM) patients have not been well studied. In this study, the detection rates of 5 putative periodontal pathogens: Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Eikenella corrodens, Treponema denticola, and Candida albicans by polymerase chain reaction (PCR) between NIDDM and non-DM adults were compared. A total of 246 adults were randomly recruited and periodontal parameters including: plaque index (P1I), gingival index (GI), probing depth (PD) and attachment level (AL) were recorded. Subgingival plaque samples were collected by sterile curettes from the most diseased and healthy sites based on PD and AL. The differences in periodontal parameters and microbiological data in healthy and diseased sites between non-DM and NIDDM patients were compared by chi-square analysis. The results showed no significant differences in age, gender, GI, P1I, PD, and prevalence of the 5 microorganisms between the NIDDM and the non-diabetic groups. However, except for A. actinomycetemcomitans, the prevalence of the periodontal microorganisms tested was significantly higher (p albicans may play important roles in the periodontitis of both NIDDM and non-DM individuals, however the etiology of periodontitis in both groups may not be different from each other.

  4. Right Time, Right Place: Building an Online Learning Community for Afterschool Practitioners

    Science.gov (United States)

    Balow, Nancy; Benard, Bonnie; Hipps, Jerry; Lauver, Sherri; McManus, John; Montgomery, Robert; Truebridge, Sara; Vitale, Alfred; Walker, Roy

    2010-01-01

    In 2008, the U.S. Department of Education awarded a contract to a team of education, youth development, and web development specialists to develop an online professional learning community for grantees in the 21st Century Community Learning Centers (21st CCLC). The online community, You for Youth (Y4Y, www.Y4Y.ed.gov) will support afterschool…

  5. Effect of visual impairment on goal-directed aiming movements in children

    NARCIS (Netherlands)

    Reimer, A.M.; Cox, R.F.A.; Boonstra, N.F.; Smits-Engelsman, B.C.M.

    2008-01-01

    This study investigated potential differences in motor control between children with a visual impairment (diagnosed albinism; n=11, mean age 8y 4mo [SD 7mo]; seven males, four females,) and children with normal vision (n=11, mean age 8y 4mo [SD 7mo]; six males, five females). Mean near visual acuity

  6. Effect of visual impairment on goal-directed aiming movements in children.

    NARCIS (Netherlands)

    Reimer, A.M.; Cox, R.F.A.; Boonstra, N.F.; Smits-Engelsman, B.C.M.

    2008-01-01

    This study investigated potential differences in motor control between children with a visual impairment (diagnosed albinism; n=11, mean age 8y 4mo [SD 7mo]; seven males, four females,) and children with normal vision (n=11, mean age 8y 4mo [SD 7mo]; six males, five females). Mean near visual acuity

  7. UTP-induced ATP release is a fine-tuned signalling pathway in osteocytes

    DEFF Research Database (Denmark)

    Kringelbach, Tina M.; Aslan, Derya; Novak, Ivana;

    2014-01-01

    intracellular calcium responses to P2 receptor agonists. These investigations demonstrated that MLO-Y4 osteocytes express functional P2Y2, P2Y4, P2Y12 and P2Y13 receptors in addition to the previously reported P2X receptors. Further, we found that osteocytes respond to nucleotides such as ATP, UTP and ADP by...

  8. AcEST: DK960915 [AcEST

    Lifescience Database Archive (English)

    Full Text Available P55665|Y4TK_RHISN Uncharacterized cyclodeaminase y4tK OS=Rhiz... 32 3.4 sp|P87515|POLN_BFV Non-structural polyprotein OS=Barmah fores...al polyprotein OS=Barmah forest virus PE=2 SV=2 Length = 2410 Score = 32.0 bits (71), Expect = 3.4 Identitie

  9. Study of a plugging microbial consortium using crude oil as sole carbon source

    Institute of Scientific and Technical Information of China (English)

    Wang Jing; Yan Guiwen; An Mingquan; Liu Jieli; Zhang Houming; Chen Yun

    2008-01-01

    A microbial consortium named Y4 capable of producing biopolymers was isolated from petroleum-contaminated soil in the Dagang Oilfield, China. It includes four bacterial strains: Y4-1 (Paenibacillus sp.), Y4-2 (Actinomadura sp.), Y4-3 (Uncultured bacterium clone) and Y4-4 (Brevibacillus sp.). The optimal conditions for the growth of the consortium Y4 were as follows: temperature about 46 ℃,pH about 7.0 and salinity about 20.0 g/L. The major metabolites were analyzed with gas chromatographymass spectrometry (GC-MS). A comparison was made between individual strains and the microbial consortium for biopolymer production in different treatment processes. The experimental results showed that the microbial consortium Y4 could produce more biopolymers than individual strains, and the reason might be attributed to the synergetic action of strains. The biopolymers were observed with optical and electron microscopes and analyzed by paper chromatography. It was found that the biopolymers produced by the microbial consortium Y4 were insoluble in water and were of reticular structure, and it was concluded that the biopolymers were cellulose. Through a series of simulation experiments with sand cores, it was found that the microbial consortium Y4 could reduce the permeability of reservoir beds, and improve the efficiency of water flooding by growing biomass and producing biopolymers.The oil recovery was enhanced by 3.5% on average. The results indicated that the consortium Y4 could be used in microbial enhanced oil recovery and play an important role in bioremediation of oil polluted environments.

  10. AcEST: BP920221 [AcEST

    Lifescience Database Archive (English)

    Full Text Available nition sp|Q9Y4A5|TRRAP_HUMAN Transformation/transcription domain-associated protein OS=Homo sapiens Align le...ficant alignments: (bits) Value sp|Q9Y4A5|TRRAP_HUMAN Transformation/transcription domain-associ... 42 5e-05..... 29 6.3 sp|Q91820|STK6_XENLA Serine/threonine-protein kinase 6 OS=Xenopu... 29 8.2 >sp|Q9Y4A5|TRRAP_HUMAN Transformation

  11. AcEST: BP914318 [AcEST

    Lifescience Database Archive (English)

    Full Text Available Definition sp|Q9Y4A5|TRRAP_HUMAN Transformation/transcription domain-associated protein OS=Homo sapiens Alig... significant alignments: (bits) Value sp|Q9Y4A5|TRRAP_HUMAN Transformation/transcription domain-associ... 11...ormation/transcription domain-associated protein OS=Homo sapiens GN=TRRAP PE=1 SV=3...lase mnmA OS=Campy... 30 9.0 sp|Q9U720|DCSA_DICDI Cellulose synthase catalytic subunit A [UDP... 30 9.0 >sp|Q9Y4A5|TRRAP_HUMAN Transf

  12. Microbial flora in orodental infections

    Directory of Open Access Journals (Sweden)

    Saini S

    2003-01-01

    Full Text Available The present study was carried out to compare the normal aerobic and anaerobic bacterial oral flora with flora from deep seated dental caries, gingivitis and adult periodontitis. All the samples belonging to both the control and study groups yielded microbes. Aerobe / Anaerobe ratio was high in normal flora (1.48 as compared to dental caries (0.9, gingivitis (0.72 and periodontitis (0.56. Ninety seven percent of orodental infections were polymicrobial and three or more microbes were found in 84% cases of study group as compared to 28% in controls. Streptococcus mutans and anaerobic lactobacilli were common in dental caries, Actinomyces and Peptostreptococcus spp. in gingivitis, Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in periodontitis.

  13. Phylogeny of the genus Haemophilus as determined by comparison of partial infB sequences

    DEFF Research Database (Denmark)

    Hedegaard, J; Okkels, H; Bruun, B;

    2001-01-01

    A 453 bp fragment of infB, the gene encoding translation initiation factor 2, was sequenced and compared from 66 clinical isolates and type strains of Haemophilus species and related bacteria. Analysis of the partial infB sequences obtained suggested that the human isolates dependent on X and V...... factor, H. influenzae, H. haemolyticus, H. aegyptius and some cryptic genospecies of H. influenzae, were closely related to each other. H. parainfluenzae constituted a heterogeneous group within the boundaries of the genus, whereas H. aphrophilus/paraphrophilus and Actinobacillus actinomycetemcomitans...... were only remotely related to the type species of the genus Haemophilus H. parahaemolyticus and H. paraphrohaemolyticus took up an intermediary position and may not belong in the genus Haemophilus sensu stricto. Ambiguous results were obtained with seven isolates tentatively identified as H. segnis...

  14. Bases farmacomicrobiológicas del tratamiento antibiótico de las enfermedades periodontales y periimplatarias Farmacobiological concepts in the antibiotic treatment of the periodontal diseases

    Directory of Open Access Journals (Sweden)

    J. Liñares

    2003-12-01

    Full Text Available La enfermedad periodontal debe considerarse un proceso infeccioso bacteriano crónico. En su etiología, no hay una única especie bacteriana implicada, sino que podríamos considerarla como una infección polimicrobiana en la que estarían implicados diversos microorganismos. Las bacterias que se han asociado más directamente con la enfermedad periodontal son Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus y Treponema denticola. Los parámetros farmacodinámicos de los antibióticos son muy útiles a la hora de seleccionar pautas posológicas. El aumento de resistencias producido en muchos periodontopatógenos en los últimos años ha relegado a algunos antibióticos a un segundo plano. Entre la gran variedad de antibióticos utilizados, se han obtenido buenas respuestas terapéuticas con amoxicilina/ácido clavulánico, metronidazol, clindamicina, doxiciclina y las combinaciones de metronidazol más amoxicilina y metronidazol más amoxicili-na/ácido clavulánico.Periodontal disease must be considered a chronic bacterial infection. It does not appear to one single bacterial species that is uniquely involved. Rather, periodontal disease seems to be a polymicrobial infection involving several organisms. The bacteria most often associated with periodontal disease are Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus y Treponema denticola. Pharmacodynamics parameters are very useful to select dosing regimens. The increase in prevalence of resistance occurred in some periodontopathogens in the last years has pushed some antibiotics into the background. Positive responses have been reported with amoxicillin/clavulanate, metronidazole, clindamycin, doxycycline and the combination therapy metronidazole plus amoxicillin and metronidazole plus amoxicillin/clavulanate.

  15. Hadron distributions at higher rapidity using the BRAHMS forward spectrometer

    Energy Technology Data Exchange (ETDEWEB)

    Moskowitz, B. [Brookhaven National Lab., Upton, NY (United States)

    1995-07-15

    Different conditions, and therefore different physics, are expected to exist over the range of rapidities at RHIC. The BRAHMS Forward Spectrometer will measure identified hadron distributions up to y=4, giving it a unique place in the RHIC experimental program.

  16. 切触变换下四阶微分方程的几何%The Geometry of the Differential Equations of the Fourth Order Under the Contact Transformations

    Institute of Scientific and Technical Information of China (English)

    陈维桓; 李海中

    1999-01-01

    用E.Cartan的等价方法,研究切触变换下四阶微分方程y(4)=f(x,y,y′,y″,y″′)的几何.%It is studied that the geometry of the differential equations of the fourth order y(4) = f(x, y,y′, y″, y′″) under contact transformations by E. Cartan's method of equivalence.

  17. Charm Physics at SuperB

    International Nuclear Information System (INIS)

    The study of Charm Decays at SuperB provide unique opportunities to understand the Standard Model and constrain new physics, both at the Y(4S), and at charm threshold. We discuss the physics potential of such measurements from the proposed SuperB experiment with 75 ab-1 of data at the Y(4S) and a subsequent run dedicated to exploiting quantum correlations at the charm threshold. (author)

  18. Antimicrobial activity against periodontopathogenic bacteria,antioxidant and cytotoxic effects of various extracts from endemic Thermopsis turcica

    Institute of Scientific and Technical Information of China (English)

    Elif; Burcu; Bali; Leyla; Acik; Glin; Akca; Meral; Sarper; Mualla; Pinar; Eli; Ferit; Avcu; Mecit; Vural

    2014-01-01

    Objective:To investigate the in vitro antimicrobial potential of Thermopsis turcica Kit Tan,Vural&Kckdk against periodoutopathogenie bacteria,its antioxidant activity and cytotoxic effect on various cancer cell lines.Methods:In vitro antimicrobial activities of elhanol.methanol,ethyl acetate(ElAc,n-hexane and water extracts of Thermopsis turcica herb against periodontopathogenic bacteria,Aggregatibacter actinnmycelemconilans ATCC 29523 and Porphyromonas gingivalis ATCC 33277 were tested by agar well diffusion,minimum inhibitory concentration(MIC)and minimal bactericidal concentration(MBC),Antioxidant properties of the extracts were evaluatod by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity and p-carotene bleaching methods.Amounts of phenolic contents of the extracts were also analysed by using the Folin-Ciocalteu reagent.Additionally,cytotoxic activity of the extracts on androgcn-insensilivc prostate cancer,androgen—sensitive prostate cancer,chronic myelogenous leukemia and acute promyelocytic leukemia bunian cancer cell lines were determined by 3-4,5-dimelhylthiazol-2-yh-2,5-diphenyltclrazolium bromide assay.Human gingival fibroblast cells were used as a control.Results:Our data showed that ELAc extract had the highest antimicrobial effect on Aggregatibacter actinomycetemitans(MIC:1.562 mg/mlMHC:3.124 mg/ml.)and Porph yromonas gingiralis(MIC:0.781 mg/mL,MBC:1.562 mg/mL).In antioxidant assays.ElAc extract exhibited also the highest radical scavenging activity[IC50=(30.0±0.3)μg/ml.]and the highest inhibition[(74.35±0.30)%]|against lineloic acide oxidation.The amount of phenolic content of it was also the highest[(l62.5±l.2)μg/mg gallic acid].In cytotoxic assay,only etbanol[IC50=(80.00±1.21)μg/ml.]and EtAc extract[IC50=(70.0±0.9)μg/ml]were toxic on acute promyeloeytic leukemia cells at 20—100μg/mL.P<0.05>.However,no toxic effect was observed on human gingival fibroblast cells

  19. Osteocyte culture in microfluidic devices.

    Science.gov (United States)

    Wei, Chao; Fan, Beiyuan; Chen, Deyong; Liu, Chao; Wei, Yuanchen; Huo, Bo; You, Lidan; Wang, Junbo; Chen, Jian

    2015-01-01

    This paper presents a microfluidic device (poly-dimethylsiloxane micro channels bonded with glass slides) enabling culture of MLO-Y4 osteocyte like cells. In this study, on-chip collagen coating, cell seeding and culture, as well as staining were demonstrated in a tubing-free manner where gravity was used as the driving force for liquid transportation. MLO-Y4 cells were cultured in microfluidic channels with and without collagen coating where cellular images in a time sequence were taken and analyzed, confirming the positive effect of collagen coating on phenotype maintaining of MLO-Y4 cells. The proliferating cell nuclear antigen based proliferation assay was used to study cellular proliferation, revealing a higher proliferation rate of MLO-Y4 cells seeded in microfluidic channels without collagen coating compared to the substrates coated with collagen. Furthermore, the effects of channel dimensions (variations in width and height) on the viability of MLO-Y4 cells were explored based on the Calcein-AM and propidium iodide based live/dead assay and the Hoechst 33258 based apoptosis assay, locating the correlation between the decrease in channel width or height and the decrease in cell viability. As a platform technology, this microfluidic device may function as a new cell culture model enabling studies of osteocytes. PMID:25713691

  20. Osteocytes up-regulate the terminal differentiation of pre-osteoblasts via gap junctions.

    Science.gov (United States)

    Nishikawa, Yoichi; Akiyama, Yuko; Yamamoto, Kiyofumi; Kobayashi, Masayuki; Watanabe, Eri; Watanabe, Nobukazu; Shimizu, Noriyoshi; Mikami, Yoshikazu; Komiyama, Kazuo

    2015-01-01

    We examined cell-to-cell interaction between pre-osteoblasts and osteocytes using MC3T3-E1 and MLO-Y4, respectively. First, GFP expressing MC3T3-E1 (E1-GFP) cells were generated to isolate the cells from co-culture with MLO-Y4. No changes were observed in the expression of osteogenic transcription factors Runx2, Osterix, Dlx5 and Msx2, but expression of alkaline phosphatase (ALP) and bone sialoprotein (BSP) in E1-GFP co-cultured with MLO-Y4 was 300-400-fold greater than that in mono-cultured E1-GFP. In addition, mineralized nodule formation was drastically increased in co-cultured E1-GFP cells compared to mono-cultured cells. Patch clamp assay showed the presence of gap junctions between E1-GFP and MLO-Y4. Furthermore, when the gap junction inhibitor carbenoxolone (CBX) was added to the culture, increased expression of ALP and BSP in E1-GFP co-cultured with MLO-Y4 was suppressed. These results suggest that gap junction detected between pre-osteoblasts and osteocytes plays an important role on the terminal differentiation of pre-osteoblasts. PMID:25450679

  1. Preliminary molecular analysis of bacterial composition in periapical lesions with primary endodontic infections of deciduous teeth

    Institute of Scientific and Technical Information of China (English)

    SHANG Jia-jian; YANG Qiu-bo; ZHAO Huan-ying; CAI Shuang; ZHOU Yan; SUN Zheng

    2013-01-01

    Background The bacterial composition of periapical lesions in deciduous teeth has not been well documented.This study was designed to explore the bacterial compositions,especially the dominant bacteria in periapical lesions using 16S rRNA sequencing.Methods Tissue samples were collected from 11 periapical lesions in deciduous teeth with primary endodontic infections.DNA was extracted from each sample and analyzed using 16S rRNA cloning and sequencing for the identification of bacteria.Results All DNA samples were positive for 16S rRNA gene PCR.One hundred and fifty-one phylotypes from 810 clones were identified to eight phyla,and each sample contained an average of 25.9 phylotypes.In addition,59 phylotypes were detected in more than two samples,and Fusobacterium (F.) nucleatum (8/11),Dialister (D.) invisus (8/11),Campylobacter (C.) gracilis (7/11),Escherichia (E.) coil DH1 (6/11),Aggregatibacter (A.) segnis (6/11),and Streptococcus (S.) mitis (6/11) were the most prevalent species.Furthermore,45 as-yet-uncultivated phylotypes were also identified.Conclusions Chronic periapical lesions in deciduous teeth contained polymicrobial infections.F.nucleatum,D.invisus,C.gracilis,E.coli DH1,A.segnis,and S.mitis were the most prevalent species detected by 16S rRNA sequencing.

  2. Microbial diversity similarities in periodontal pockets and atheromatous plaques of cardiovascular disease patients.

    Directory of Open Access Journals (Sweden)

    Wagner Serra e Silva Filho

    Full Text Available BACKGROUND AND OBJECTIVE: The immune and infectious alterations occurring in periodontitis have been shown to alter the development and severity of cardiovascular disease. One of these relationships is the translocation of oral bacteria to atheroma plaques, thereby promoting plaque development. Thus, the aim of this study was to assess, by 16s cloning and sequencing, the microbial diversity of the subgingival environment and atheroma plaques of patients concomitantly suffering from periodontitis and obstructive coronary artery atherosclerosis (OCAA. METHODS: Subgingival biofilm and coronary balloons used in percutaneous transluminal coronary angioplasty were collected from 18 subjects presenting with generalized moderate to severe periodontitis and OCAA. DNA was extracted and the gene 16S was amplified, cloned and sequenced. RESULTS: Significant differences in microbial diversity were observed between both environments. While subgingival samples mostly contained the phylum Firmicutes, in coronary balloons, Proteobacteria (p<0.05 was predominant. In addition, the most commonly detected genera in coronary balloons were Acinetobacter, Alloprevotella, Pseudomonas, Enterobacter, Sphingomonas and Moraxella, while in subgingival samples Porphyromonas, Filifactor, Veillonella, Aggregatibacter and Treponema (p<0.05 were found. Interestingly, 17 identical phylotypes were found in atheroma and subgingival samples, indicating possible bacterial translocation between periodontal pockets and coronary arteries. CONCLUSION: Periodontal pockets and atheromatous plaques of cardiovascular disease patients can present similarities in the microbial diversity.

  3. Path and ridge regression analysis of seed yield and seed yield components of Russian wildrye (Psathyrostachys juncea Nevski) under field conditions

    DEFF Research Database (Denmark)

    Wang, Quanzhen; Zhang, Tiejun; Cui, Jian;

    2011-01-01

    numbers per spikelet (Y4) and seed weight (Y5) were counted and the Z were determined in field experiments from 2003 to 2006 via big sample size. Y1 was the most important seed yield component describing the Z and Y2 was the least. The total direct effects of the Y1, Y3 and Y5 to the Z were positive while...... Y4 and Y2 were weakly negative. The total effects (directs plus indirects) of the components were positively contributed to the Z by path analyses. The seed yield components Y1, Y2, Y4 and Y5 were significantly (P<0.001) correlated with the Z for 4 years totally, while in the individual years, Y2...... most important that, via ridge regression, a steady algorithm model between Z and the five yield components was founded, which can be closely estimated the seed yield via the components....

  4. Biological characteristics of two lactic acid bacteria from orange-spotted grouper(Epinephelus coioides)larvae%2株鱼源乳酸菌的生物学特性研究

    Institute of Scientific and Technical Information of China (English)

    杨红玲; 孙云章; 叶继丹; 常建波; 陈政强; 林文燕

    2008-01-01

    [目的]研究2株鱼源乳酸菌的生物学特性,为石斑鱼高效益生菌株的筛选提供试验依据.[方法] 体外检测乳酸菌EA-1和Y4-2产乳酸及超氧化物歧化酶(SOD)的能力,以及2菌株对不同pH值、胆盐、胃蛋白酶和高温的耐受能力.[结果] 菌株EA-1和Y4-2均具有较强的产乳酸能力,培养液中的乳酸含量在96 h内随培养时间延长呈上升趋势,EA-1产乳酸速度相对较快.菌株EA-1和Y4-2均能在培养24 h后产生活性稳定的SOD,培养48 h后EA-1产生的SOD活性显著高于Y4-2(P<0.05).菌株EA-1和Y4-2 经pH 2.5~4.5处理4 h后存活数维持在106 mL-1以上,菌株Y4-2对pH 1.5低酸环境的耐受能力强于EA-1.2菌株在1 g/L胆盐质量浓度下存活数均较高;胆盐质量浓度达到2 g/L时,EA-1和Y4-2的存活数可维持在105 mL-1以上,提示2菌株对≤2 g/L的胆盐具有一定的耐受性.经4~10 g/L的蛋白酶处理30 min后,EA-1和Y4-2的存活数均维持在106 mL-1以上.经60 ℃处理30 min后EA-1和Y4-2的存活数均维持在106 mL-1以上, Y4-2对80 ℃高温的耐受能力较EA-1强,说明EA-1和Y4-2有一定的热耐受性.[结论] EA-1和Y4-2产乳酸和SOD能力均较强,且对低pH值、高胆盐、胃蛋白酶和高温具有一定的耐受能力.

  5. Several Diophantine Equations in Some Rings of Integers of Quadratic Imaginary Fields

    Institute of Scientific and Technical Information of China (English)

    Kejian Xu; Yongliang Wang

    2007-01-01

    In this paper, it is proved that the Diophantine equation x4 - y4 = z2 has no non-trivial coprime solutions in the rings of integers of quadratic imaginary fields Q(√-d)for d=11, 19, 43, 67, 163, which implies that the Fermat equation x4+y4=z4 has no nontrivial solutions in these fields either. Then all the solutions of the Pocklington equation x4-x2y2+y4 = (-1)σz2(σ=0 or 1) in the ring of integers of Q(√-11) are determined,and as an application, the result is applied to K2 of a field.

  6. The use of genomic DNA fingerprinting in studies of the epidemiology of bacteria in periodontitis.

    Science.gov (United States)

    Genco, R J; Loos, B G

    1991-07-01

    Recent studies of microbial epidemiology emphasizing the genetic organization and distribution of organisms associated with orofacial infections have led to new insights into the possible origins of pathogenicity. Studies into genetic heterogeneity, acquisition and transmission of these organisms have been markedly advanced by the utilization of the powerful technique of genomic DNA fingerprinting. Characteristic fingerprints for each bacterial isolate can be produced by cleavage of high molecular weight genomic DNA by restriction endonucleases. It is assumed that each DNA fingerprint represents a clonal type. In this report, we review and analyze studies of the epidemiology of bacteria associated with orofacial infections with an emphasis on periodontal disease. Studies of nontypable (NT) Haemophilus influenzae associated with recurrent otitis media illustrate the utility of this technique. DNA fingerprinting clearly demonstrates genetic heterogeneity of NT H. influenzae isolates, and clonality of infection of any individual. Furthermore, DNA fingerprinting has shown that the same clonal type is seen in siblings concurrently suffering from otitis media, suggesting horizontal transmission within the family. Studies of mutans Streptococci also show extensive genetic heterogeneity and show vertical transmission of a predominant clonal type only from mother to infant, but not from father to infant. Studies of Actinobacillus actinomycetemcomitans show considerable genetic heterogeneity among monkey isolates. Thus far, three clonal types have been reported with DNA fingerprinting among isolates from periodontal patients, but additional genetic heterogeneity can be found using specific DNA fragments as probes in hybridization experiments. Intrafamilial transmission of A. actinomycetemcomitans has been demonstrated. Porphyromonas (Bacteroides) gingivalis shows extensive genetic heterogeneity and case reports suggest clonal infection of any one individual. In contrast

  7. Quantitative PCR analysis of salivary pathogen burden in periodontitis

    Directory of Open Access Journals (Sweden)

    Aino eSalminen

    2015-10-01

    Full Text Available Our aim was to investigate the value of salivary concentrations of four major periodontal pathogens and their combination in diagnostics of periodontitis. The Parogene study included 462 dentate subjects (mean age 62.9±9.2 years with coronary artery disease diagnosis who underwent an extensive clinical and radiographic oral examination. Salivary levels of four major periodontal bacteria were measured by quantitative real-time PCR. Median salivary concentrations of P. gingivalis, T. forsythia, and P. intermedia, as well as the sum of the concentrations of the four bacteria, were higher in subjects with moderate to severe periodontitis compared to subjects with no to mild periodontitis. Median salivary A. actinomycetemcomitans concentrations did not differ significantly between the subjects with no to mild periodontitis and subjects with moderate to severe periodontitis. In logistic regression analysis adjusted for age, gender, diabetes, and the number of teeth and implants, high salivary concentrations of P. gingivalis, T. forsythia, and P. intermedia were significantly associated with moderate to severe periodontitis. When looking at different clinical and radiographic parameters of periodontitis, high concentrations of P. gingivalis and T. forsythia were significantly associated with the number of 4-5 mm periodontal pockets, ≥ 6 mm pockets, and alveolar bone loss (ABL. High level of T. forsythia was associated also with bleeding on probing (BOP. The combination of the four bacteria, i.e. the bacterial burden index, was associated with moderate to severe periodontitis with an odds ratio (OR of 2.40 (95% CI 1.39–4.13. When A. actinomycetemcomitans was excluded from the combination of the bacteria, the OR was improved to 2.61 (95% CI 1.51–4.52. The highest odds ratio 3.59 (95% CI 1.94–6.63 was achieved when P. intermedia was further excluded from the combination and only the levels of P. gingivalis and T. forsythia were used. Salivary

  8. Periodontal profile and presence of periodontal pathogens in young African-Americans from Salvador, BA, Brazil Condição periodontal e presença de patógenos periodontais em uma população jovem de brasileiros afro-descendentes

    Directory of Open Access Journals (Sweden)

    Ligia Valéria Victor

    2008-06-01

    Full Text Available This cross-sectional study evaluated the periodontal status and the presence of periodontopathogens in 132 young, black ethnic subjects who live in Salvador/Bahia-Brazil and have never smoked. Periodontal Probing Depth (PPD, Clinical Attachment Level (CAL, Plaque Index (PI and Gingival Index (GI were measured and analyzed by ANOVA and Wilcoxon tests (pO objetivo do presente estudo foi avaliar a condição periodontal e presença de periodonto-patógenos em uma amostra de 132 jovens não fumantes, afro-descendentes, residentes em Salvador/Bahia-Brasil. Profundidade de Sondagem (PS, Nível Clínico de Inserção (NCI, Índice de Placa (IP e Índice Gengival (IG foram mensurados e analisados pelos testes ANOVA e Wilcoxon (p<0,05 em função do gênero e idade dos indivíduos. A. actinomycetemcomitans, P. gingivalis, E. corrodens e F. nucleatum foram identificados por PCR e analisados por ANOVA, Wilcoxon e t de Student (p<0,05. Foram observados valores médios de PS e NCI de 2,18 e 1,0mm respectivamente. Os parâmetros clínicos avaliados não sofreram influência dos fatores gênero e idade (p<0,05. E. corrodens foi a bactéria mais prevalente (95.45%, seguido de F. nucleatum (68.18%, A. actinomycetemcomitans (45.45% e P. gingivalis (40.9%. Não foi observada associação entre os patógenos periodontais pesquisados com o gênero e idade dos indivíduos (p<0,05. PS, NCI e IP não estiveram associados a presença de P. gingivalis, todavia para o parâmetro IG este mostrou alta freqüência entre os indivíduos que não alocaram P. gingivalis. Nessa população jovem de brasileiros afro-descendentes um percentual (96,96% elevado de indivíduos apresentou pelo menos um patógeno periodontal, mas mostraram-se clinicamente saudáveis. Outras investigações são necessaries para avaliar a real influencia da presença dessas espécies bacterianas.

  9. A new member of the 4-methylideneimidazole-5-one–containing aminomutase family from the enediyne kedarcidin biosynthetic pathway

    Science.gov (United States)

    Huang, Sheng-Xiong; Lohman, Jeremy R.; Huang, Tingting; Shen, Ben

    2013-01-01

    4-Methylideneimidazole-5-one (MIO)-containing aminomutases catalyze the conversion of l-α-amino acids to β-amino acids with either an (R) or an (S) configuration. l-Phenylalanine and l-tyrosine are the only two natural substrates identified to date. The enediyne chromophore of the chromoprotein antitumor antibiotic kedarcidin (KED) harbors an (R)-2-aza-3-chloro-β-tyrosine moiety reminiscent of the (S)-3-chloro-5-hydroxy-β-tyrosine moiety of the C-1027 enediyne chromophore, the biosynthesis of which uncovered the first known MIO-containing aminomutase, SgcC4. Comparative analysis of the KED and C-1027 biosynthetic gene clusters inspired the proposal for (R)-2-aza-3-chloro-β-tyrosine biosynthesis starting from 2-aza-l-tyrosine, featuring KedY4 as a putative MIO-containing aminomutase. Here we report the biochemical characterization of KedY4, confirming its proposed role in KED biosynthesis. KedY4 is an MIO-containing aminomutase that stereospecifically catalyzes the conversion of 2-aza-l-tyrosine to (R)-2-aza-β-tyrosine, exhibiting no detectable activity toward 2-aza-l-phenylalanine or l-tyrosine as an alternative substrate. In contrast, SgcC4, which stereospecifically catalyzes the conversion of l-tyrosine to (S)-β-tyrosine in C-1027 biosynthesis, exhibits minimal activity with 2-aza-l-tyrosine as an alternative substrate but generating (S)-2-aza-β-tyrosine, a product with the opposite stereochemistry of KedY4. This report of KedY4 broadens the scope of known substrates for the MIO-containing aminomutase family, and comparative studies of KedY4 and SgcC4 provide an outstanding opportunity to examine how MIO-containing aminomutases control substrate specificity and product enantioselectivity. PMID:23633564

  10. Osteocytes produce interferon-β as a negative regulator of osteoclastogenesis.

    Science.gov (United States)

    Hayashida, Chiyomi; Ito, Junta; Nakayachi, Mai; Okayasu, Mari; Ohyama, Yoko; Hakeda, Yoshiyuki; Sato, Takuya

    2014-04-18

    Osteoclastogenesis is controlled by osteocytes; osteocytic osteoclastogenesis regulatory molecules are largely unknown. We searched for such factors using newly developed culture methods. Our culture system mimics the three-dimensional cellular structure of bone, consisting of collagen gel-embedded osteocytic MLO-Y4 cells, stromal ST2 cells on the gel as bone lining cells, and bone marrow cells. The gel-embedded MLO-Y4 cells inhibited the osteoclastogenesis induced by 1,25(OH)2D3 without modulating receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) production by ST2 cells, despite MLO-Y4 cells supported osteoclastogenesis in the absence of ST2 cells. In the bone marrow cell culture, the conditioned medium from MLO-Y4 cells decreased the capability of osteoclastic differentiation from the cells induced by macrophage colony-stimulating factor. This decreased capability was concomitant with an increase in protein kinase R mRNA expression and an inhibition of c-Fos translation. These changes were partially normalized by the simultaneous addition of an anti-interferon (IFN)-β neutralizing antibody to MLO-Y4 cell conditioned medium. To study primary osteocytes, we prepared non-osteocytic cell-free osteocyte-enriched bone fragments (OEBFs). When osteoclast precursors were induced by macrophage colony-stimulating factor in the presence of OEBFs, the generated cells exhibited a diminished capacity for osteoclastogenesis. OEBFs prepared from OPG-knock-out mice exhibited a similar effect, indicating OPG-independent inhibition. The addition of anti-IFN-β neutralizing antibody during the co-culture with OEBFs partially recovered the osteoclastogenic potential of the generated cells. The MLO-Y4 cells and OEBFs expressed IFN-β mRNA. Although osteocytic RANKL is known to be important for osteoclastogenesis, our data suggest that osteocytes also produce IFN-β as an inhibitor of osteoclastogenesis. PMID:24610813

  11. AcEST: BP920479 [AcEST

    Lifescience Database Archive (English)

    Full Text Available .. 30 5.3 sp|P38996|NAB3_YEAST Nuclear polyadenylated RNA-binding protein ... 30 5.4 sp|Q80YV3|TRRAP_MOUSE Transformation.../transcription domain-associ... 30 7.0 sp|Q9Y4A5|TRRAP_HUMAN Transformation...SAPPMSHQPPPPQQQQQQQQQQ 778 >sp|Q80YV3|TRRAP_MOUSE Transformation/transcription domain-associated protein OS=...PIQWLAWIPQL 1926 >sp|Q9Y4A5|TRRAP_HUMAN Transformation/transcription domain-assoc

  12. Beauty physics at e+ e- colliders

    International Nuclear Information System (INIS)

    Beauty physics to be performed in the next decade at the resonances Y(4S) and Z0 are compared. Large similarities are found in the physics program and the reconstruction techniques of Z0 and asymmetric Y(4S) colliders. The physics potential of the latter is found to be superior at equal luminosity to a symmetric machine, provided a large enough boost (≥ 5). Z0 machines will probably be the main source of the rich BS0 physics during that period

  13. The FERRUM Project: laboratory-measured transition probabilities for Cr II

    OpenAIRE

    Gurell, J.; Nilsson, Hampus; Engström, Lars; Lundberg, Hans; Blackwell-Whitehead, Richard; Nielsen, K. E.; Mannervik, S.

    2010-01-01

    Aims: We measure transition probabilities for Cr II transitions from the z ^4H_J, z ^2D_J, y ^4F_J, and y ^4G_J levels in the energy range 63000 to 68000 cm^{-1}. Methods: Radiative lifetimes were measured using time-resolved laser-induced fluorescence from a laser-produced plasma. In addition, branching fractions were determined from intensity-calibrated spectra recorded with a UV Fourier transform spectrometer. The branching fractions and radiative lifetimes were combined to yield accurate ...

  14. Efecto de la adición de proteasas en el proceso madurativo de los embutidos crudos curados

    OpenAIRE

    Díaz Rubio, Olga

    1994-01-01

    Se ha realizado un estudio con el fin de conocer si la adición de proteasas constituye un método útil para potenciar el sabor y aroma y/o acortar el periodo de maduración de los embutidos. Para ello se han elaborado distintos lotes de embutidos a los que se añadieron tres proteasas en distintas dosis: pronasa e de streptomyces griseus (600 y 6.000 unidades enzimáticas), asprtil proteinasa acida de aspergillus orizae (800 y 4.500 unidades) y papaina de carica papaya (800 y 4.500 unidades). Fin...

  15. CCL7 Is a Protective Factor Secreted by Mechanically Loaded Osteocytes

    OpenAIRE

    Kitase, Y.; Lee, S.; Gluhak-Heinrich, J; Johnson, M.L.; Harris, S.E.; Bonewald, L. F.

    2014-01-01

    In a search for factors up-regulated by mechanical strain in osteocytes, we discovered that chemokine (C-C motif) ligand 7 (CCL7), a chemotactic myokine, was highly expressed in MLO-Y4 osteocyte-like cells. Although MLO-Y4 cells secrete potent chemotactic factors for osteoclast precursors, CCL7 was not responsible for this activity. CCL7 was increased in osteocytes in response to tooth movement in vivo. Since mechanical loading plays a crucial role in maintaining osteocyte viability, CCL7 was...

  16. Osteocyte culture in microfluidic devices

    OpenAIRE

    Wei, Chao; Fan, Beiyuan; Chen, Deyong; Liu, Chao; Wei, Yuanchen; Huo, Bo; You, Lidan; Wang, Junbo; Chen, Jian

    2015-01-01

    This paper presents a microfluidic device (poly-dimethylsiloxane micro channels bonded with glass slides) enabling culture of MLO-Y4 osteocyte like cells. In this study, on-chip collagen coating, cell seeding and culture, as well as staining were demonstrated in a tubing-free manner where gravity was used as the driving force for liquid transportation. MLO-Y4 cells were cultured in microfluidic channels with and without collagen coating where cellular images in a time sequence were taken and ...

  17. CILIA-LIKE STRUCTURES AND POLYCYSTIN-1 IN OSTEOBLASTS/OSTEOCYTES AND ASSOCIATED ABNORMALITIES IN SKELETOGENESIS AND RUNX2 EXPRESSION*

    OpenAIRE

    Xiao, Zhousheng; Zhang, Shiqin; Mahlios, Josh; Zhou, Gan; Magenheimer, Brenda S.; Guo, Dayong; Dallas, Sarah L.; Maser, Robin; Calvet, James P.; Bonewald, Lynda; Quarles, Leigh Darryl

    2006-01-01

    We examined the osteoblast/osteocyte expression and function of polycystin-1 (PC1), a transmembrane protein that is a component of the polycystin-2 (PC2)-ciliary mechanosensor complex in renal epithelial cells. We found that MC3T3-E1 osteoblasts and MLO-Y4 osteocytes express transcripts for PC1, PC2 and the ciliary proteins Tg737 and Kif3a. Immunohistochemical analysis detected cilia-like structures in MC3T3-E1 osteoblastic and MLO-Y4 osteocyte-like cell lines as well as primary osteocytes an...

  18. FGF and ERK signaling coordinately regulate mineralization-related genes and play essential roles in osteocyte differentiation

    OpenAIRE

    Kyono, Ai; Avishai, Nanthawan; Ouyang, Zhufeng; Landreth, Gary E.; Murakami, Shunichi

    2011-01-01

    To examine the roles of FGF and ERK MAPK signaling in osteocyte differentiation and function, we performed microarray analyses using the osteocyte cell line MLO-Y4. This experiment identified a number of mineralization-related genes that were regulated by FGF2 in an ERK MAPK dependent manner. Real-time PCR analysis indicated that FGF2 upregulates Ank, Enpp1, Mgp, Slc20a1, and Dmp1 in MLO-Y4 cells. Consistent with this observation, the selective FGF receptor inhibitor PD173074 decreased Ank, E...

  19. Putative periodontopathic bacteria and herpesviruses in pregnant women: a case-control study

    Science.gov (United States)

    Lu, Haixia; Zhu, Ce; Li, Fei; Xu, Wei; Tao, Danying; Feng, Xiping

    2016-01-01

    Little is known about herpesvirus and putative periodontopathic bacteria in maternal chronic periodontitis. The present case-control study aimed to explore the potential relationship between putative periodontopathic bacteria and herpesviruses in maternal chronic periodontitis.Saliva samples were collected from 36 pregnant women with chronic periodontitis (cases) and 36 pregnant women with healthy periodontal status (controls). Six putative periodontopathic bacteria (Porphyromonas gingivalis [Pg], Aggregatibacer actinomycetemcomitans [Aa], Fusobacterium nucleatum [Fn], Prevotella intermedia [Pi], Tannerella forsythia [Tf], and Treponema denticola [Td]) and three herpesviruses (Epstein-Barr virus [EBV], human cytomegalovirus [HCMV], and herpes simplex virus [HSV]) were detected. Socio-demographic data and oral health related behaviors, and salivary estradiol and progesterone levels were also collected. The results showed no significant differences in socio-demographic background, oral health related behaviors, and salivary estradiol and progesterone levels between the two groups (all P > 0.05). The detection rates of included periodontopathic microorganisms were not significantly different between the two groups (all P > 0.05), but the coinfection rate of EBV and Pg was significantly higher in the case group than in the control group (P = 0.028). EBV and Pg coinfection may promote the development of chronic periodontitis among pregnant women. PMID:27301874

  20. Influencia del estrógeno en la enfermedad periodontal: revisión de literatura

    Directory of Open Access Journals (Sweden)

    V.F.C. Pazmino

    2015-08-01

    Full Text Available La etiología de la enfermedad periodontal está bien definida, dentro de los agentes etiológicos que la causan podemos citar algunos microorganismos subgingivales como: Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus, Actinobacillus actinomycetemcomitans y espiroquetas. La susceptibilidad del huésped a estos agentes bacterianos también tiene un papel importante dentro del progreso y prevalencia de la enfermedad periodontal. Dentro de los factores de riesgo asociados con la enfermedad periodontal tenemos: el aumento de edad, presencia de biofilm dental o placa bacteriana patogénica, alteración del estado inmunológico, deficiencia nutricional, el uso de medicamentos como corticoides, género, estrés, tabaco, factores genéticos y condiciones sistémicas, dentro de estas se incluyen alteraciones de neutrófilos, diabetes, embarazo, osteoporosis y hormonales. La enfermedad periodontal puede ser exacerbada por determinados factores sistémicos como la deficiencia o aumento de hormonas como estrógeno y progesterona. La presente revisión de literatura tiene por objeto dilucidar la influencia del estrógeno en la enfermedad periodontal.

  1. Anaerobic infections in the head and neck region.

    Science.gov (United States)

    Tabaqchali, S

    1988-01-01

    Anaerobic bacteria form the predominant flora of the oral cavity, outnumbering facultative organisms by 10-1,000: 1. The type of anaerobic bacteria and their concentration depend on the anatomical site and the degree of anaerobiosis in the different sites in the mouth. Three groups of anaerobic bacteria inhabit the oral cavity; the strict anaerobes, the moderate anaerobes, and the microaerophilic group of organisms. The majority of anaerobic bacterial infections occurring in the region of the mouth, head and neck are caused by the commensal flora. These infections include dental and periodontal disease where the predominant organisms are Bacteroides species, Veillonella, Bifidobacteria, Peptococcus, Peptostreptococcus and Propionibacterium species. More recently, Bacteroides endontalis has been isolated from a periapical abscess of endodontal origin and B. gingivalis, B. intermedius, Haemophilus actinomycetemcomitans and Wollinella species in chronic periodontal disease. Treponema species and other strict anaerobes are seen in smears of severe periodontal disease and acute necrotising gingivitis, but have not yet been isolated in pure culture. Until such time, their role in disease remains uncertain. Fusobacterium nucleatum is specially associated with severe orofacial infections which may extend into the mediastinum. Other anaerobic infections include chronic otitis media, chronic sinusitis and mastoiditis, and brain abscess. Treatment of these conditions should include the use of beta-lactamase resistant antimicrobials, such as clindamycin or one of the nitroimidazoles with penicillin.

  2. Chronic gingivitis: the prevalence of periodontopathogens and therapy efficiency.

    Science.gov (United States)

    Igic, M; Kesic, L; Lekovic, V; Apostolovic, M; Mihailovic, D; Kostadinovic, L; Milasin, J

    2012-08-01

    The purpose of this study was to determine the level of gingival inflammation and the prevalence of periodontopathogenic microorganisms in adolescents with chronic gingivitis, as well as to compare the effectiveness of two approaches in gingivitis treatment-basic therapy alone and basic therapy + adjunctive low-level laser therapy (LLLT). After periodontal evaluation, the content of gingival pockets of 140 adolescents with gingivitis was analyzed by multiplex PCR for the presence of P. gingivalis, A. actinomycetemcomitans, T. forsythensis and P. intermedia. Subsequent to bacteria detection, the examinees were divided into two groups with homogenous clinical and microbiological characteristics. Group A was subjected to basic gingivitis therapy, and group B underwent basic therapy along with adjunctive LLLT. A statistically significant difference between the values of plaque-index (PI) and sulcus bleeding index (SBI) before and after therapy was confirmed in both groups (pgingivitis should be regarded as a sign for dentists to foster more effective oral health programs. LLLT appears to be beneficial as adjuvant to basic therapy.

  3. МИКРОБИОЛОГИЧЕСКОЕ ОБОСНОВАНИЕ ВЫБОРА БАЗИСНОГО МАТЕРИАЛА ЗУБОЧЕЛЮСТНЫХ ЛЕЧЕБНЫХ АППАРАТОВ БОЛЬНЫМ С ПОСЛЕОПЕРАЦИОННЫМИ ДЕФЕКТАМИ ВЕРХНЕЙ ЧЕЛЮСТИ

    OpenAIRE

    Царев, Виктор; Арутюнов, Анатолий; Седракян, Айрапет; Орлова, Ольга; Спиранде, Ирина

    2009-01-01

    В эксперименте in vitro изучали адгезивную способность микроорганизмов Streptococcus sanguis, Streptococcus milleri, Streptococcus mutans, Enterococcus faecalis, Actinomyces naeslundii, Candida albicans, Veillonella parvula, Prevotella intermedia, Porphyromonas gingivalis, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans к базисным пластмассам стомакрил и фторакс, структурированным двумя способами горячей полимеризацией и СВЧ-полимеризацией. Пациентам с дефектами челюстей при нал...

  4. Evaluation of In-Vitro Antibacterial Acitivity of Cinnamomum zeylanicum Extract on Different Microorganisms of the Dental Plaque

    Directory of Open Access Journals (Sweden)

    Fouad Hussain M. H. Al-Bayaty

    2015-10-01

    Full Text Available The present study was conducted to evaluate the antibacterial activity of aqueous and ethanol extracts of Cinnaomum zeylanicum on different types of dental plaque microorganisms. Screening study was perforned to detect the potential antibacterial activity against S. aureus, E. coli, S. mutans, L. casei, B. fragilis, A. actinomycemtemcomitans and dental plaque pool samples. From the screening test, values of Minimum Inhibitory Concentration (MIC were determined. The lowest MIC value was 25 mg/ml of aqueous and 12.5 mg/ml of ethanol extract for S. aureus. The highest MIC values were seen in A. actinomycetemcomitans and dental plaque aerobic pool samples with 300 mg/ml of aqueous extract and 150 mg/ml of ethanol extract. The MIC values for aqueous extracts ranged from 25 to 300 mg/ml whereas for fixed plant concentration test, showed the strongest inhibition effect for all the organisms tested. Generally, the ethanol extract of Cinnamomum zeylanicum demonstrated a stronger antibacterial activity compared to the aqueous extract. This study also compared the antibacterial activity of chlorhexidine with that of the plant extracts. Chlorhexidine showed a higher antibacterial effect on the microorganisms, with almost all organisms inhibited.

  5. Influence of hormonal contraceptives on microbial flora of gingival sulcus.

    Science.gov (United States)

    Klinger, G; Eick, S; Klinger, G; Pfister, W; Gräser, T; Moore, C; Oettel, M

    1998-06-01

    To determine a possible influence of two different hormonal contraceptives on bacterial microflora of gingival sulcus, subgingival plaque samples of 29 healthy women aged between 20 and 32 years were investigated bacteriologically before subjects took a contraceptive and 10 and 20 days after subjects started the medication. In 14 women, and oral contraceptive containing 0.02 mg ethinyl estradiol and 0.15 mg desogestrel (preparation A) was used, and 15 women took a contraceptive containing 0.03 mg ethinyl estradiol and 2.00 mg dienogest (preparation B) daily over 21 days. There were no changes in clinical parameters of the teeth investigated during 3 weeks of the study. The periodontopathogenic bacteria Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans were never detected throughout the study. On the other hand, the periodontopathogenic species Prevotella intermedia was found in plaque samples of 22 women. The content of this microorganism showed only a little change between the pretreatment period and plaque sampling after 10 days of contraceptive treatment, but a striking increase occurred after 20 days of contraceptive treatment, especially in the preparation A group. In this respect, there was a significant difference between preparations A and B. PMID:9693397

  6. thermo-stable alpha-amylase(S) from irradiated microbial origin utilizing agricultural and environmental wastes under solid state fermentation conditions

    International Nuclear Information System (INIS)

    an investigation concerning the production of thermo-stable α-amylases by thermophilic bacterial and fungal isolates has been undertaken. nine thermophilic bacteria and five teen fungi were isolated from different localities viz. phyllosphere of water hyacinth, different desert plants leaves, fermented dough, oven dust, garbage , and soil. their amylolytic activities were tested by dinitrosalicylic acid color reagent (Dns) method when grown on some environmental pollutants (garbage and water hyacinth) as well as industrial wastes (Bagasse, biscuit, corn flex and dough residues ) as the sole carbon source at 65o C for bacterial and at 50o C for fungal isolates . isolates No. B1,B2,B5,B6,B7,B8,B9, and F4,F6,F8,F12,F13 and F15, exhibited the highest α -amylase production when grown on water hyacinth, while B4,F3,F11 and F13, on dough ; (B3,F9 and F10 ) on bagasse and ( F1,F2,F5,F7,F11 and F14) on garbage. Out of the nine identified bacterial isolated, only two isolates viz; actinobacillus actinomycetemcomitans, B1 and strepto bacillus moniliformis, B7, exhibited the ability to produce high percentages of α amylases at 55o C (while still able to produce the enzyme within 45-70o C)

  7. Comparative cytotoxicity of periodontal bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Stevens, R.H.; Hammond, B.F.

    1988-11-01

    The direct cytotoxicity of sonic extracts (SE) from nine periodontal bacteria for human gingival fibroblasts (HGF) was compared. Equivalent dosages (in terms of protein concentration) of SE were used to challenge HGF cultures. The cytotoxic potential of each SE was assessed by its ability to (1) inhibit HGF proliferation, as measured by direct cell counts; (2) inhibit 3H-thymidine incorporation in HGF cultures; or (3) cause morphological alterations of the cells in challenged cultures. The highest concentration (500 micrograms SE protein/ml) of any of the SEs used to challenge the cells was found to be markedly inhibitory to the HGFs by all three of the criteria of cytotoxicity. At the lowest dosage tested (50 micrograms SE protein/ml); only SE from Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, and Fusobacterium nucleatum caused a significant effect (greater than 90% inhibition or overt morphological abnormalities) in the HGFs as determined by any of the criteria employed. SE from Capnocytophaga sputigena, Eikenella corrodens, or Wolinella recta also inhibited cell proliferation and thymidine incorporation at this dosage; however, the degree of inhibition (5-50%) was consistently, clearly less than that of the first group of three organisms named above. The SE of the three other organisms tested (Actinomyces odontolyticus, Bacteroides intermedius, and Streptococcus sanguis) had little or no effect (0-10% inhibition) at this concentration. The data suggest that the outcome of the interaction between bacterial components and normal resident cells of the periodontium is, at least in part, a function of the bacterial species.

  8. Eikenella corrodens: Patogénesis y aspectos clínicos

    Directory of Open Access Journals (Sweden)

    Rubén Darío Jaramillo

    2006-09-01

    Full Text Available El ambiente microbiológico oral es único y tiene una dinámica compleja. Se calcula que cerca de 500 especies de bacterias habitan la cavidad oral humana, y alrededor de 22 géneros son los predominantes. Las bacterias que se aíslan con más frecuencia de los sitios infectados de la cavidad oral, y que son también patógenos potenciales, forma un grupo pequeño de microorganismos gramnegativos, entre los que se incluyen los siguientes: Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Campylobacter spp., Capnocytophoga spp., Eikenella corrodens, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia y el grupo-Streptococcus milleri. E. corrodens se reconoce como un microbio patógeno oportunista en la cavidad oral; también, puede causar infecciones extra orales, como un agente infeccioso solo o en combinaciones con diversas bacterias. La presente revisión, con un énfasis en E. corrodens, mostrará varios aspectos de sus características microbiológicas y bioquímicas, también se sistematizan y discuten el conocimiento actual sobre los mecanismos de patogénesis, como los lipopolisacáridos, proteínas externas de membrana, complejo de adesina, pili y otros; infecciones oral y extra oral; factores predisponentes; endocarditis; osteomielitis, infecciones intra-abdominales; diagnóstico y agentes antimicrobianos.

  9. Eikenella corrodens: Patogénesis y aspectos clínicos.

    Directory of Open Access Journals (Sweden)

    Rubén Darío Jaramillo

    2009-11-01

    Full Text Available El ambiente microbiológico oral es único y tiene una dinámica compleja. Se calcula que cerca de 500 especies de bacterias habitan la cavidad oral humana, y alrededor de 22 géneros son los predominantes. Las bacterias que se aíslan con más frecuencia de los sitios infectados de la cavidad oral, y que son también patógenos potenciales, forma un grupo pequeño de microorganismos gramnegativos, entre los que se incluyen los siguientes: Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Campylobacter spp., Capnocytophoga spp., Eikenella corrodens, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia y el grupo-Streptococcus milleri. E. corrodens se reconoce como un microbio patógeno oportunista en la cavidad oral; también, puede causar infecciones extra orales, como un agente infeccioso solo o en combinaciones con diversas bacterias. La presente revisión, con un énfasis en E. corrodens, mostrará varios aspectos de sus características microbiológicas y bioquímicas, también se sistematizan y discuten el conocimiento actual sobre los mecanismos de patogénesis, como los lipopolisacáridos, proteínas externas de membrana, complejo de adesina, pili y otros; infecciones oral y extra oral; factores predisponentes; endocarditis; osteomielitis, infecciones intra-abdominales; diagnóstico y agentes antimicrobianos.

  10. Dissemination of periodontal pathogens in the bloodstream after periodontal procedures: a systematic review.

    Directory of Open Access Journals (Sweden)

    Anna Carolina Ratto Tempestini Horliana

    Full Text Available BACKGROUND: To date, there is no compilation of evidence-based information associating bacteremia and periodontal procedures. This systematic review aims to assess magnitude, duration, prevalence and nature of bacteremia caused by periodontal procedures. STUDY DESIGN: Systematic Review. TYPES OF STUDIES REVIEWED: MEDLINE, EMBASE and LILACS databases were searched in duplicate through August, 2013 without language restriction. Observational studies were included if blood samples were collected before, during or after periodontal procedures of patients with periodontitis. The methodological quality was assessed in duplicate using the modified Newcastle-Ottawa scale (NOS. RESULTS: Search strategy identified 509 potentially eligible articles and nine were included. Only four studies demonstrated high methodological quality, whereas five were of medium or low methodological quality. The study characteristics were considered too heterogeneous to conduct a meta-analysis. Among 219 analyzed patients, 106 (49.4% had positive bacteremia. More frequent bacteria were S. viridans, A. actinomycetemcomitans P. gingivalis, M. micros and species Streptococcus and Actinomyces, although identification methods of microbiologic assays were different among studies. CLINICAL IMPLICATIONS: Although half of the patients presented positive bacteremia after periodontal procedures, accurate results regarding the magnitude, duration and nature of bacteremia could not be confidentially assessed.

  11. Inhibitory effect of quercetin on periodontal pathogens in vitro.

    Science.gov (United States)

    Geoghegan, F; Wong, R W K; Rabie, A B M

    2010-06-01

    Actinobacillus actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg) are bacteria strongly associated with early onset, progressive and refractory periodontal disease and associated alveolar bone loss. Quercetin is a flavonoid found in many foods including apples, onions and tea. The aim of this study was to evaluate the effect of quercetin on in vitro growth of periodontal pathogens Aa and Pg. For comparison, quercetin's effect on several oral microbes was also evaluated. Different concentrations of quercetin solution were added to calibrated suspensions of Aa and Pg. All suspensions were incubated for 1, 3, 6, and 24 h in an anaerobic chamber at 37 degrees C. At each time point, selected dilutions from each culture broth were plated on blood agar plates. Colonies appearing on blood agar plates were visually counted on 3 days for Aa and 5 days for Pg. Minimum inhibitory concentrations of both periodontal pathogens were also determined. Both periodontal bacteria showed a significant decrease (p < 0.05) in viable counts after 1 h. No colony forming units of Pg could be observed after 24 h. The results suggest that quercetin possesses significant antimicrobial properties on periodontal pathogens in vitro. PMID:19957242

  12. 新目标英语八年级(上)Unit7 STEP BY STEP随堂通

    Institute of Scientific and Technical Information of China (English)

    高克军

    2009-01-01

    1.根据句意及首字母提示补全单词。1. Please p_ the apples before you eat them. 2. It's wasteful to p_ the clean water. 3. I don't like pure milk, but I like y. 4.-How many i do you need to make fruit salad?

  13. Existence of solutions of nonlinear two-point boundary value problems for 4nth-order nonlinear differential equation

    Institute of Scientific and Technical Information of China (English)

    高永馨

    2002-01-01

    Studies the existence of solutions of nonlinear two point boundary value problems for nonlinear 4n-th-order differential equation y(4n)= f( t,y,y' ,y",… ,y(4n-1) ) (a) with the boundary conditions g2i(y(2i) (a) ,y(2i+1) (a)) = 0,h2i(y(2i) (c) ,y(2i+1) (c)) = 0, (I= 0,1,…,2n - 1 ) (b) where the functions f, gi and hi are continuous with certain monotone properties. For the boundary value problems of nonlinear nth order differential equation y(n) = f(t,y,y',y",… ,y(n-1)) many results have been given at the present time. But the existence of solutions of boundary value problem (a), (b) studied in this paper has not been covered by the above researches. Moreover, the corollary of the important theorem in this paper, I.e. Existence of solutions of the boundary value problem. Y(4n) = f(t,y,y',y",… ,y(4n-1) ) a2iy(2i) (at) + a2i+1y(2i+1) (a) = b2i ,c2iy(2O ( c ) + c2i+1y(2i+1) ( c ) = d2i, ( I = 0,1 ,…2n - 1) has not been dealt with in previous works.

  14. Primer registro de Serratospiculum tendo (Nematoda: Diplotriaenidae) para el Perú

    OpenAIRE

    Gomez-Puerta, Luis A.; Pedro A. Ospina; Ramirez, Mercy G.; Nelly G. Cribillero

    2014-01-01

    Reportamos por primera vez la presencia del nematodo, Serratospiculum tendo Nitzsch, 1819, parasitando los sacos aéreos de un halcón peregrino (Falco peregrinus Tunstall, 1771). Seis nematodos (2 machos y 4 hembras) fueron colectados e identificados como S. tendo. El hallazgo de este nematodo constituye el primer registro en el Perú.

  15. Primer registro de Serratospiculum tendo (Nematoda: Diplotriaenidae para el Perú

    Directory of Open Access Journals (Sweden)

    Luis A. Gomez-Puerta

    2014-05-01

    Full Text Available Reportamos por primera vez la presencia del nematodo, Serratospiculum tendo Nitzsch, 1819, parasitando los sacos aéreos de un halcón peregrino (Falco peregrinus Tunstall, 1771. Seis nematodos (2 machos y 4 hembras fueron colectados e identificados como S. tendo. El hallazgo de este nematodo constituye el primer registro en el Perú.

  16. Response Switching Process in Children with Attention-Deficit-Hyperactivity Disorder on the Novel Continuous Performance Test

    Science.gov (United States)

    Inoue, Yuki; Inagaki, Masumi; Gunji, Atsuko; Furushima, Wakana; Kaga, Makiko

    2008-01-01

    We examined the effects of previous trials on subsequent trials on performance in the continuous performance test (CPT) in children with attention-deficit-hyperactivity disorder (ADHD). Thirty-five non-medicated children with ADHD (31 males, four females; mean age 9y 10mo [SD 2y 4mo]) and 33 comparison children (20 males, 13 females; mean age 10y…

  17. Diagnóstico sobre las violéncias de género 2.0

    OpenAIRE

    Vilà Baños, Ruth; Donoso Vázquez, Trinidad; Prado Soto, María Nieves

    2015-01-01

    Informe diridido a los claustros de los centros de Educación Secundaria. Detección de necesidades de la juventud de 3o y 4o de ESO ante las violencias de género 2.0. Fundamentación de una Intervención en los centros de secundaria.

  18. FERMENTATION EFFICIENCY OF YEAST ISOLATES AND THE EFECT OF NUTRIENT SUPPLEMENTS

    Directory of Open Access Journals (Sweden)

    HELEN SOIBAM1, AYAM VICTOR SINGH2, IVI CHAKRABORTY3

    2014-12-01

    Full Text Available ABSTRACT: Wine is one of the end products of fermentation of respiratory substrates, while, yeast is one of the principal fermenting microorganisms. Changes in the substrate composition and yeast strains could vary in the quality of the wine. Two yeast isolates (Y4 and Y7 from date palm juice and standard yeast “Y33”,an isolate of Saccharomyces cerevisiae (MTCC-170 from Institute of MicrobiAl Technology, IMTECH, Chandigarh where used for studying the comparative fermentation of Molasses without nutrient supplementation, and separately with nutrient supplementation by KH2PO4 and NaNO3 @ 1g/l. Though, with nutrient supplement KH2PO4, Y33 yields higher alcohol%, both Y4 and Y7 strains produce wine with better efficiency and acceptability without the nutrient supplements unlike Y33 strains. All the three strains impart better flavour and colour in the medium without nutrient supplement in terms of hedonic scale. Further, Y4 yields higher alcohol than Y7 and Y33. Therefore, palm juice yeast isolates particularly, Y4 strains show the highest overall fermentation efficiency and acceptability among the three yeasts isolates, moreover, since its highest efficiency occurs in the absence of nutrient supplementation, unlike the standard strain, it can be recommendedas a better substitute of the standard Y33 strains. KEY WORDS: Fermentation, Yeast,  Nutrient supplements.

  19. A monoclonal antibody against KCNK9 K+ channel extracellular domain inhibits tumour growth and metastasis

    Science.gov (United States)

    Sun, Han; Luo, Liqun; Lal, Bachchu; Ma, Xinrong; Chen, Lieping; Hann, Christine L.; Fulton, Amy M.; Leahy, Daniel J.; Laterra, John

    2016-01-01

    Two-pore domain potassium (K2P) channels act to maintain cell resting membrane potential—a prerequisite for many biological processes. KCNK9, a member of K2P family, is implicated in cancer, owing to its overexpression in human tumours and its ability to promote neoplastic cell survival and growth. However, KCNK9's underlying contributions to malignancy remain elusive due to the absence of specific modulators. Here we describe the development of monoclonal antibodies against the KCNK9 extracellular domain and their functional effects. We show that one antibody (Y4) with the highest affinity binding induces channel internalization. The addition of Y4 to KCNK9-expressing carcinoma cells reduces cell viability and increases cell death. Systemic administration of Y4 effectively inhibits growth of human lung cancer xenografts and murine breast cancer metastasis in mice. Evidence for Y4-mediated carcinoma cell autonomous and immune-dependent cytotoxicity is presented. Our study reveals that antibody-based KCNK9 targeting is a promising therapeutic strategy in KCNK9-expressing malignancies. PMID:26842342

  20. 14 CFR Appendix A to Part 150 - Noise Exposure Maps

    Science.gov (United States)

    2010-01-01

    ..., business and professional Y Y 25 30 N N Wholesale and retail—building materials, hardware and farm... Communication Y Y 25 30 N N Manufacturing and Production Manufacturing, general Y Y Y(2) Y(3) Y(4) N... outdoor noise problems. (2) Measures to achieve NLR 25 dB must be incorporated into the design...

  1. Evaluación terapéutica de los derivados sanguíneos en coagulopatías, en la ciudad de bogotá

    OpenAIRE

    Rodríguez Torres, Jaime; Mendoza Posada, César; Velasco Ch., Álvaro; Camacho Durán, Álvaro; Vargas del Valle, Alfonso

    2011-01-01

    Se hace una evaluación de la eficacia de los primeros críoprecipitados y PRP, preparados en el Banco Nacional de Sangre, con los que se trataron 12 pacientes con diferentes coagulopatías incluyendo 8 hemofilias, 2 coagulopatías por mordedura de serpiente y 4 púrpuras tromcitopénicos, con resultados satisfactorios.

  2. Dicty_cDB: VSD594 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 624-220200-032-B08 BT0624 Homo sapiens cDNA, mRNA sequence. 40 2.6 1 BG731932 |BG731932.1 ps07c01.y4 Trichinella... spiralis ML CMVsport jasmer Trichinella spiralis cDNA 5' similar to contains

  3. Dicty_cDB: SLJ322 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 2 BG732108 |BG732108.1 ps20c12.y4 Trichinella spiralis ML CMVsport jasmer Trichinella spiralis cDNA 5' simil...54 0.002 1 BG353063 |BG353063.1 ps20c12.y1 Trichinella spiralis ML CMVsport jasmer Trichinella spiralis cDNA

  4. Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

    OpenAIRE

    Mattinzoli, Deborah; Messa, Piergiorgio; Corbelli, Alessandro; Ikehata, Masami; Mondini, Anna; Zennaro, Cristina; Armelloni, Silvia; Li, Min; Giardino, Laura; Rastaldi, Maria Pia

    2014-01-01

    The need for osteocyte cultures is well known to the community of bone researchers; isolation of primary osteocytes is difficult and produces low cell numbers. Therefore, the most widely used cellular system is the osteocyte-like MLO-Y4 cell line.

  5. Diamagnetic levitation causes changes in the morphology, cytoskeleton, and focal adhesion proteins expression in osteocytes.

    Science.gov (United States)

    Qian, A R; Wang, L; Gao, X; Zhang, W; Hu, L F; Han, J; Li, J B; Di, S M; Shang, Peng

    2012-01-01

    Diamagnetic levitation technology is a novel simulated weightless technique and has recently been applied in life-science research. We have developed a superconducting magnet platform with large gradient high magnetic field (LG-HMF), which can provide three apparent gravity levels, namely, μg (diamagnetic levitation), 1g, and 2g for diamagnetic materials. In this study, the effects of LG-HMF on the activity, morphology, and cytoskeleton (actin filament, microtubules, and vimentin intermediate filaments) in osteocyte - like cell line MLO-Y4 were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) methods, hematoxylin-eosin (HE) staining, and laser scanning confocal microscopy (LSCM), respectively. The changes induced by LG-HMF in distribution and expression of focal adhesion (FA) proteins, including vinculin, paxillin, and talin in MLO-Y4 were determined by LSCM and Western blotting. The results showed that LG-HMF produced by superconducting magnet had no lethal effects on MLO-Y4. Compared to control, diamagnetic levitation (μg) affected MLO-Y4 morphology, nucleus size, cytoskeleton architecture, and FA proteins distribution and expression. The study indicates that osteocytes are sensitive to altered gravity and FA proteins (vinculin, paxillin, and talin) may be involved in osteocyte mechanosensation. The diamagnetic levitation may be a novel ground-based space-gravity simulator and can be used for biological experiment at cellular level. PMID:21216704

  6. Low-intensity pulsed ultrasound regulates proliferation and differentiation of osteoblasts through osteocytes.

    Science.gov (United States)

    Li, Lei; Yang, Zheng; Zhang, Hai; Chen, Wenchuan; Chen, Mengshi; Zhu, Zhimin

    2012-02-10

    Low-intensity pulsed ultrasound (LIPUS) has been used as a safe and effective modality to enhance fracture healing. As the most abundant cells in bone, osteocytes orchestrate biological activities of effector cells via direct cell-to-cell contacts and by soluble factors. In this study, we have used the osteocytic MLO-Y4 cells to study the effects of conditioned medium from LIPUS-stimulated MLO-Y4 cells on proliferation and differentiation of osteoblastic MC3T3-E1 cells. Conditioned media from LIPUS-stimulated MLO-Y4 cells (LIPUS-Osteocyte-CM) were collected and added on MC3T3-E1 cell cultures. MC3T3-E1 cells cultured in LIPUS-Osteocyte-CM demonstrated a significant inhibition of proliferation and an increased alkaline phosphatase activity. The results of PGE(2) and NO assay showed that LIPUS could enhance PGE(2) and NO secretion from MLO-Y4 cells at all time points within 24h after LIPUS stimulation. We conclude that LIPUS regulates proliferation and differentiation of osteoblasts through osteocytes in vitro. Increased secretion of PGE(2) from osteocytes may play a role in this effect. PMID:22266313

  7. AcEST: DK961140 [AcEST

    Lifescience Database Archive (English)

    Full Text Available yclodeaminase y4tK OS=Rhiz... 32 3.1 sp|P87515|POLN_BFV Non-structural polyprotein OS=Barmah forest...LN_BFV Non-structural polyprotein OS=Barmah forest virus PE=2 SV=2 Length = 2410 Score = 32.0 bits (71), Exp

  8. AcEST: DK959322 [AcEST

    Lifescience Database Archive (English)

    Full Text Available rized cyclodeaminase y4tK OS=Rhiz... 32 3.2 sp|P87515|POLN_BFV Non-structural polyprotein OS=Barmah forest v...AFPELGEVIAGVKTGRTRESDITIADLTGTG 306 >sp|P87515|POLN_BFV Non-structural polyprotein OS=Barmah forest virus PE

  9. AcEST: DK959748 [AcEST

    Lifescience Database Archive (English)

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  10. Dexamethasone-induced apoptosis of osteocytic and osteoblastic cells is mediated by TAK1 activation.

    Science.gov (United States)

    Ding, Heyuan; Wang, Tao; Xu, Dongli; Cha, Bingbing; Liu, Jun; Li, Yiming

    2015-05-01

    Increased apoptosis of osteoblasts and osteocytes is the main mechanism of glucocorticoid (GC)-induced osteonecrosis. In the current study, we investigated whether dexamethasone (Dex)-induced osteoblastic and osteocytic cell apoptosis is mediated through activation of transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1), and whether TAK1 inhibition could promote survival opposing the deleterious effects of Dex. We found that TAK1 was activated by Dex in both osteocytic MLO-Y4 and osteoblastic OB-6 cells, which was prevented by two known anti-oxidants N-acetylcysteine (NAC) and ebselen. TAK1 inhibitors, including LYTAK1 and 5Z-7-oxozeaenol (57-OZ), inhibited Dex-induced apoptosis of MLO-Y4 and OB-6 cells. Meanwhile shRNA-mediated knockdown of TAK1 also suppressed Dex-induced damages to MLO-Y4 and OB-6 cells. On the other hand, exogenously over-expressing TAK1 enhanced Dex-induced MLO-Y4 and OB-6 cell apoptosis. At the molecular level, we found that TAK1 mediated Dex-induced pro-apoptotic Pyk2-JNK activation. Inhibition or silencing of TAK1 almost abolished Pyk2-JNK phosphorylations by Dex in MLO-Y4 and OB-6 cells. TAK1 over-expression, on the other hand, increased Dex's activity on Pyk2-JNK phosphorylations in above cells. We conclude that part of the pro-apoptotic actions of Dex on osteoblastic and osteocytic cells are mediated through TAK1 activation, and that inhibition of TAK1 might protect from GC-induced damages to osteoblasts and osteocytes. PMID:25753204

  11. Low-intensity pulsed ultrasound regulates proliferation and differentiation of osteoblasts through osteocytes

    Energy Technology Data Exchange (ETDEWEB)

    Li, Lei, E-mail: geraldleelei@163.com [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu (China); Yang, Zheng [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu (China); Zhang, Hai [Department of Restorative Dentistry, School of Dentistry, University of Washington, Seattle, WA (United States); Chen, Wenchuan [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu (China); Chen, Mengshi [Department of Biomechanics, Sichuan University, Chengdu (China); Zhu, Zhimin, E-mail: hxzhimin@163.com [State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu (China)

    2012-02-10

    Highlights: Black-Right-Pointing-Pointer CM from LIPUS-stimulated osteocytes inhibits proliferation of osteoblasts. Black-Right-Pointing-Pointer CM from LIPUS-stimulated osteocytes enhances differentiation of osteoblasts. Black-Right-Pointing-Pointer LIPUS stimulates MLO-Y4 cells to secrete PGE{sub 2} and NO. -- Abstract: Low-intensity pulsed ultrasound (LIPUS) has been used as a safe and effective modality to enhance fracture healing. As the most abundant cells in bone, osteocytes orchestrate biological activities of effector cells via direct cell-to-cell contacts and by soluble factors. In this study, we have used the osteocytic MLO-Y4 cells to study the effects of conditioned medium from LIPUS-stimulated MLO-Y4 cells on proliferation and differentiation of osteoblastic MC3T3-E1 cells. Conditioned media from LIPUS-stimulated MLO-Y4 cells (LIPUS-Osteocyte-CM) were collected and added on MC3T3-E1 cell cultures. MC3T3-E1 cells cultured in LIPUS-Osteocyte-CM demonstrated a significant inhibition of proliferation and an increased alkaline phosphatase activity. The results of PGE{sub 2} and NO assay showed that LIPUS could enhance PGE{sub 2} and NO secretion from MLO-Y4 cells at all time points within 24 h after LIPUS stimulation. We conclude that LIPUS regulates proliferation and differentiation of osteoblasts through osteocytes in vitro. Increased secretion of PGE{sub 2} from osteocytes may play a role in this effect.

  12. The ecological proportion of indigenous bacterial populations in saliva is correlated with oral health status.

    Science.gov (United States)

    Takeshita, Toru; Nakano, Yoshio; Kumagai, Takashi; Yasui, Masaki; Kamio, Noriaki; Shibata, Yukie; Shiota, Susumu; Yamashita, Yoshihisa

    2009-01-01

    To obtain deeper insights into the etiology of oral disease, an understanding of the composition of the surrounding bacterial environments that lead to health or disease is required, which is attracting increasing attention. In this study, the bacterial compositions in the saliva of 200 subjects aged 15-40 years were depicted as peak patterns by terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes. The subjects were classified into three clusters by partitioning around medoids clustering based on their T-RFLP profiles, and the clinical oral health parameters of the clusters were compared. The clustering of the T-RFLP profiles in this study was mainly based on differences in the abundance distribution of the dominant terminal restriction fragments (TRFs) detected in most of the subjects. Predicted from the sizes of the TRFs, the characteristically more predominant members of each were Prevotella and Veillonella species in cluster I; Streptococcus species in cluster II and Neisseria, Haemophilus or Aggregatibacter species and Porphyromonas species in cluster III. The parameters associated with periodontal disease were significantly different among the clusters. Clusters I and II had a higher percentage of sites of periodontal pockets greater than 4 mm than cluster III, and cluster I contained sites exhibiting bleeding on probing more often than cluster II or III; no significant differences were observed in other parameters. These results suggest that the abundance distribution of commensal bacteria in saliva is correlated with periodontal health, and might be involved in the susceptibility of an individual to periodontal disease. PMID:18830275

  13. Maturation of Oral Microbiota in Children with or without Dental Caries.

    Directory of Open Access Journals (Sweden)

    Pernilla Lif Holgerson

    Full Text Available The aim of this longitudinal study was to evaluate the oral microbiota in children from age 3 months to 3 years, and to determine the association of the presence of caries at 3 years of age.Oral biofilms and saliva were sampled from children at 3 months (n = 207 and 3 years (n = 155 of age, and dental caries was scored at 3 years of age. Oral microbiota was assessed by culturing of total lactobacilli and mutans streptococci, PCR detection of Streptococcus mutans and Streptococcus sobrinus, 454 pyrosequencing and HOMIM (Human Oral Microbe Identification Microarray microarray detection of more then 300 species/ phylotypes. Species richness and taxa diversity significantly increased from 3 months to 3 years. Three bacterial genera, present in all the 3-month-old infants, persisted at 3 years of age, whereas three other genera had disappeared by this age. A large number of new taxa were also observed in the 3-year-olds. The microbiota at 3 months of age, except for lactobacilli, was unrelated to caries development at a later age. In contrast, several taxa in the oral biofilms of the 3-year-olds were linked with the presence or absence of caries. The main species/phylotypes associated with caries in 3-year-olds belonged to the Actinobaculum, Atopobium, Aggregatibacter, and Streptococcus genera, whereas those influencing the absence of caries belonged to the Actinomyces, Bergeyella, Campylobacter, Granulicatella, Kingella, Leptotrichia, and Streptococcus genera.Thus, during the first years of life, species richness and taxa diversity in the mouth increase significantly. Besides the more prevalent colonization of lactobacilli, the composition of the overall microbiota at 3 months of age was unrelated to caries development at a later age. Several taxa within the oral biofilms of the 3-year-olds could be linked to the presence or absence of caries.

  14. Spatiotemporal properties of intracellular calcium signaling in osteocytic and osteoblastic cell networks under fluid flow.

    Science.gov (United States)

    Jing, Da; Lu, X Lucas; Luo, Erping; Sajda, Paul; Leong, Pui L; Guo, X Edward

    2013-04-01

    Mechanical stimuli can trigger intracellular calcium (Ca(2+)) responses in osteocytes and osteoblasts. Successful construction of bone cell networks necessitates more elaborate and systematic analysis for the spatiotemporal properties of Ca(2+) signaling in the networks. In the present study, an unsupervised algorithm based on independent component analysis (ICA) was employed to extract the Ca(2+) signals of bone cells in the network. We demonstrated that the ICA-based technology could yield higher signal fidelity than the manual region of interest (ROI) method. Second, the spatiotemporal properties of Ca(2+) signaling in osteocyte-like MLO-Y4 and osteoblast-like MC3T3-E1 cell networks under laminar and steady fluid flow stimulation were systematically analyzed and compared. MLO-Y4 cells exhibited much more active Ca(2+) transients than MC3T3-E1 cells, evidenced by more Ca(2+) peaks, less time to the 1st peak and less time between the 1st and 2nd peaks. With respect to temporal properties, MLO-Y4 cells demonstrated higher spike rate and Ca(2+) oscillating frequency. The spatial intercellular synchronous activities of Ca(2+) signaling in MLO-Y4 cell networks were higher than those in MC3T3-E1 cell networks and also negatively correlated with the intercellular distance, revealing faster Ca(2+) wave propagation in MLO-Y4 cell networks. Our findings show that the unsupervised ICA-based technique results in more sensitive and quantitative signal extraction than traditional ROI analysis, with the potential to be widely employed in Ca(2+) signaling extraction in the cell networks. The present study also revealed a dramatic spatiotemporal difference in Ca(2+) signaling for osteocytic and osteoblastic cell networks in processing the mechanical stimulus. The higher intracellular Ca(2+) oscillatory behaviors and intercellular coordination of MLO-Y4 cells provided further evidences that osteocytes may behave as the major mechanical sensor in bone modeling and remodeling

  15. Induction of DNA-strand breaks after X-irradiation in murine bone cells of various differentiation capacities

    Science.gov (United States)

    Lau, Patrick; Hellweg, Christine E.; Kirchner, Simone; Baumstark-Khan, Christa

    During longterm space missions, astronauts suffer from the loss of minerals especially from weightbearing bones due to prolonged sojourn under microgravity. In addition to weightlessness, exposure to cosmic ionization radiation is another space related factor endangering health and productivity of astronauts. In order to elucidate changes in bone cell metabolism induced by ionizing radiation, ground-based bone cell models have been developed. The differentiation level of the bone cells may influence their radiation sensitivity. Therefore, our cell model comprises a collection of immortalized murine pre-osteoblast, osteoblast and osteocyte cell lines representing discrete stages of differentiation: the subclones 4 and 24 of the osteoblast cell line MC3T3-E1, the osteoblast cell line OCT-1 and the osteocyte cell line MLO-Y4 display varying potential to produce mineralized bone matrix upon incubation with ascorbic acid and β-glycerophosphate (osteogenic medium). The MLO-Y4 cells showed the highest and subclone 24 the lowest proliferation rate. The most intense von Kossa reaction after culture in osteogenic medium was observed in subclone 4, indicating mineralized bone matrix. The bone cell markers alkaline phosphatase and osteocalcin were determined to further characterize the differentiation stage. All cell lines expressed osteocalcin, as determined by reverse transcriptase polymerase chain reaction. The activity of alkaline phosphatase was highest in the cell line OCT-1 and very low in MLO-Y4 and S4. The peculiarity of the markers suggests a characterization of OCT-1 and S24 as preosteoblast, S4 as (mature) osteoblast, and MLO-Y4 as osteocyte. Survival after exposure to X-rays was determined using the colony forming ability test. The resulting dose-effect relationships revealed normal radiation sensitivity (compared to human fibroblasts). Cell clone specific variations (subclones 4 and 24) in the radiation sensitivity may be due to the differentiation level. The

  16. Estudio microbiológico de la periimplantitis: Presentación de 9 casos clínicos Microbiological study of the peri-implantitis: presentation of 9 clinical case

    Directory of Open Access Journals (Sweden)

    M. Quinteros Borgarello

    2000-12-01

    Full Text Available Objetivos: La periimplantitis es un proceso inflamatorio de los tejidos que rodean a un implante sometido a carga que produce la pérdida de soporte óseo. En este trabajo se muestran los resultados del estudio microbiológico y del antibiograma realizado a partir del exudado y/o del tejido periimplantario de 16 implantes funcionales con periimplantitis de 9 pacientes que fueron tratados en nuestro servicio. Material y método: Se estudiaron 16 periimplantitis observadas en 9 pacientes; de 14 de ellas se obtuvieron muestras con puntas de papel estériles que se introdujeron en la bolsa periimplantaria. En 2 implantes las muestras procedian de tejido de granulación periimplantario. Inmediatamente después de ser obtenidas, se colocaban en placas de agar-chocolate con brain heart infusion para proceder a su cultivo. Resultados: Los especímenes bacterianos aislados fueron: Stomatococcus, Prevotella oralis, Peptostreptococcus y Fusobacterium nucleatum. En 9 muestras fue imposible aislar una bacteria predominante debido a la complejidad de la flora. Respecto al resto de muestras, la bacteria predominante fue Stomatococcus en 3, Prevotella oralis en 1,Peptostreptococcus en 1 y Fusobacterium nucleatum en 2. Los antibiogramas mostraron una mayor sensibilidad a la asociación de la amoxicilina con el ácido clavulánico, comparada con la amoxicilina, el metronidazol o una combinación de estos dos últimos antimicrobianos. Conclusiones: En este trabajo las bacterias asociadas más frecuentemente a la periimplantitis fueron: Stomatococcus, Prevotella oralis, Peptostreptococcus y Fusobacterium nucleatum. En ningún caso se aislaron cepas de Actinobacillus actinomycetemcomitans. Los posibles factores de riesgo asociados a periimplantitis en nuestra serie fueron los implantes recubiertos con hidroxiapatita, implantes de 3,25mm de diámetro y la localización más distal del implante en las prótesis que rehabilitaban extremos libres edéntulos superiores

  17. O tabagismo como fator de risco para as doenças periodontais: aspectos microbiológicos

    Directory of Open Access Journals (Sweden)

    GAETTI-JARDIM JÚNIOR Elerson

    1998-01-01

    Full Text Available O fumo é considerado importante fator predisponente para muitas doenças, incluindo-se as periodontopatias. Desde que as doenças periodontais representam a inter-relação entre os fatores de virulência da microbiota subgengival sobre um hospedeiro susceptível, foi objetivo avaliar a freqüência de isolamento de três periodontopatógenos em indivíduos sadios e pacientes com doença periodontal, fumantes ou não, com níveis variados de higiene bucal; verificar a relação entre o número de microrganismos produtores de sulfeto de hidrogênio na placa subgengival de fumantes e não fumantes e sua condição clínica. Foram examinados 189 pacientes e indivíduos sadios, dos quais 60 foram selecionados para análise microbiológica. O índice de placa foi registrado de acordo com o índice de O'Leary e os espécimes de placa subgengival coletados e processados de acordo com SLOTS35 (1982. A identificação dos isolados foi obtida pelas suas características morfocelulares, morfocoloniais e bioquímico-fisiológicas. Verificou-se que a freqüência de isolamento dos bastonetes anaeróbios produtores de pigmento negro, Fusobacterium nucleatum e bactérias produtoras de sulfeto de hidrogênio foi similar entre fumantes e não fumantes, sendo mais elevada nos pacientes com doença periodontal. Já Actinobacillus actinomycetemcomitans foi isolado mais freqüentemente em sadios fumantes do que sadios não fumantes.

  18. Artificial neural networks for the diagnosis of aggressive periodontitis trained by immunologic parameters.

    Directory of Open Access Journals (Sweden)

    Georgios Papantonopoulos

    Full Text Available There is neither a single clinical, microbiological, histopathological or genetic test, nor combinations of them, to discriminate aggressive periodontitis (AgP from chronic periodontitis (CP patients. We aimed to estimate probability density functions of clinical and immunologic datasets derived from periodontitis patients and construct artificial neural networks (ANNs to correctly classify patients into AgP or CP class. The fit of probability distributions on the datasets was tested by the Akaike information criterion (AIC. ANNs were trained by cross entropy (CE values estimated between probabilities of showing certain levels of immunologic parameters and a reference mode probability proposed by kernel density estimation (KDE. The weight decay regularization parameter of the ANNs was determined by 10-fold cross-validation. Possible evidence for 2 clusters of patients on cross-sectional and longitudinal bone loss measurements were revealed by KDE. Two to 7 clusters were shown on datasets of CD4/CD8 ratio, CD3, monocyte, eosinophil, neutrophil and lymphocyte counts, IL-1, IL-2, IL-4, INF-γ and TNF-α level from monocytes, antibody levels against A. actinomycetemcomitans (A.a. and P.gingivalis (P.g.. ANNs gave 90%-98% accuracy in classifying patients into either AgP or CP. The best overall prediction was given by an ANN with CE of monocyte, eosinophil, neutrophil counts and CD4/CD8 ratio as inputs. ANNs can be powerful in classifying periodontitis patients into AgP or CP, when fed by CE values based on KDE. Therefore ANNs can be employed for accurate diagnosis of AgP or CP by using relatively simple and conveniently obtained parameters, like leukocyte counts in peripheral blood. This will allow clinicians to better adapt specific treatment protocols for their AgP and CP patients.

  19. Non-inflammatory destructive periodontal disease: a clinical, microbiological, immunological and genetic investigation

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Repeke

    2012-02-01

    Full Text Available Periodontitis comprises a group of multifactorial diseases in which periodontopathogens accumulate in dental plaque and trigger host chronic inflammatory and immune responses against periodontal structures, which are determinant to the disease outcome. Although unusual cases of non-inflammatory destructive periodontal disease (NIDPD are described, their pathogenesis remains unknown. A unique NIDPD case was investigated by clinical, microbiological, immunological and genetic tools. The patient, a non-smoking dental surgeon with excessive oral hygiene practice, presented a generalized bone resorption and tooth mobility, but not gingival inflammation or occlusion problems. No hematological, immunological or endocrine alterations were found. No periodontopathogens (A. actinomycetemcomitans, P. gingivalis, F. nucleatum and T. denticola or viruses (HCMV, EBV-1 and HSV-1 were detected, along with levels of IL-1β and TNF-a in GCF compatible with healthy tissues. Conversely ALP, ACP and RANKL GCF levels were similar to diseased periodontal sites. Genetic investigation demonstrated that the patient carried some SNPs, as well HLA-DR4 (*0404 and HLA-B27 alleles, considered risk factors for bone loss. Then, a less vigorous and diminished frequency of toothbrushing was recommended to the patient, resulting in the arrest of alveolar bone loss, associated with the return of ALP, ACP and RANKL in GCF to normality levels. In conclusion, the unusual case presented here is compatible with the previous description of NIDPD, and the results that a possible combination of excessive force and frequency of mechanical stimulation with a potentially bone loss prone genotype could result in the alveolar bone loss seen in NIDPD.

  20. Detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method.

    Science.gov (United States)

    Urbán, Edit; Terhes, Gabriella; Radnai, Márta; Gorzó, István; Nagy, Elisabeth

    2010-06-01

    To culture facultative and strict anaerobic bacteria is a well-established method for analyzing subgingival plaque samples. Micro-IDent and micro-IDent Plus (HAIN Lifescience GmbH, Nehren, Germany) tests are two commercially available rapid PCR-based methods for the identification and quantification of putative periodontopathogen bacteria. In this study, we compared these commercial PCR-based hybridization methods with conventional anaerobic culture technique. A total of 36 subgingival plaque samples were collected from periodontal pockets of pregnant women with chronic localized periodontitis. Aliquots of these samples were evaluated with species-specific probes provided by micro-IDent and micro-IDent Plus tests simultaneously, and from the same samples anaerobic and capnophylic bacteria were cultured on selective media. The overall agreement between both methods was excellent for Eubacterium nodatum, Tannerella forsythia and Porphyromonas gingivalis (97-92%), fair for Capnocytophaga sp, Eikenella corrodens, Actinobacillus actinomycetemcomitans, and Prevotella intermedia (91-89%) and poor for Fusobacterium nucleatum, Parvimonas micra (Micromonas micros), and Campylobacter rectus (86-78%). Discrepancies in the results may be explained by inability of culture method to distinguish between closely related taxa (e.i P. intermedia/Prevotella. nigrescens), and problems of keeping periodontopathogen bacteria viable, which is required for successful detection by standard culture method. Nucleic acid-based methods may replace cultivation method as frequently used methods in microbiological diagnosis of progressive periodontitis, thus micro-IDent and micro-IDent Plus tests can be recommended where culture of periodontopathogenic bacteria is not performed in routine microbiology laboratories to analyze subgingival plaque samples.

  1. Pulp microbiology of complete teeth with idiopathic apical lesions.

    Directory of Open Access Journals (Sweden)

    Patricia Rodríguez

    2009-11-01

    Full Text Available Introduction: Periapical changes named as lesions, in teeth with full crown integrity and without history of trauma, do not show a clear aetiology. Objective: To determine the presence of microorganisms in pulp dental tissue will clarify the cause of its death and therefore the damage to periodontal tissues. Materials and methods: From people between 10 and 39 years old, 23 teeth were selected. The samples were taken with paper points and 0.8 sterile files, and were transported in VMGA III medium, to be processed in the following 24 hours after they were taken and sowed in Brucella-agar. Results: The most affected teeth were upper central incisors, 43.8%. From the 23 studied teeth, microbiological grow was seen on 20 teeth. The following microorganisms species were identified: Fusobacterium spp., 25%, Eubacterium spp., 15%; Peptostreptococcus spp., 10%; Campylobacter spp., 10%; gram negative enteric bacteria, 10%; Porphyromonas gingivalis, 10%; Prevotella intermedia, 5%; Eikenellia corrodens, 5%; Dialister pneumosintes, 5%; and yeasts, 5%. There was no growing evidence of Actinomyces actinomycetemcomitans, Tanerella forsythensis and Streptococcus β  hemolytic. Discussion and conclusions: Sound pulp dental tissue is sterile; an injury over it will cause its inflammation, degeneration, death and bacterial contamination. Results in the present study clearly show the presence of microorganisms in closed apical dental lesions of endodontic origin. In same manner, it was seen that a great part of microorganisms species found can be regarded as periodontal pathogens. This could suggest a management with an endodontic, a periodontic and a pharmacological combined treatment.

  2. Relationship between invasion of the periodontium by periodontal pathogens and periodontal disease: a systematic review.

    Science.gov (United States)

    Mendes, Luzia; Azevedo, Nuno Filipe; Felino, António; Pinto, Miguel Gonçalves

    2015-01-01

    Bacterial invasion of the periodontal tissues has been suggested as a relevant step in the etiopathogenesis of periodontal disease. However, its exact importance remains to be defined. The present systematic review assessed the scientific evidence concerning the relationship between the quality or quantity of periodontal microbiota in periodontal tissues and development of periodontal disease. The databases Medline-PubMed, Cochrane-CENTRAL, ISI Web of Knowledge and SCOPUS were searched, up to January 2014. Studies that reported evaluation of periodontal pathogens invasion on human tissues were selected. The screening of 440 title/abstracts elected 26 papers for full-text reading. Twenty three papers were subsequently excluded because of insufficient data or a study protocol not related to the objectives of this systematic review. All included studies were case-control studies that evaluated intracellular or adherent bacteria to epithelial cells from periodontal pockets versus healthy sulci. Study protocols presented heterogeneity regarding case and control definitions and methodological approaches for microbial identification. No consistent significant differences were found related to the presence/absence or proportion of specific periopathogens across the studies, as only one study found statistically significant differences regarding the presence of A. actinomycetemcomitans (p = 0.043), T. forsythia (P < 0.001), P. intermedia (P < 0.001), C. ochracea (P < 0.001) and C. rectus (P = 0.003) in epithelial cells from periodontal pockets vs. healthy sulci. All studies reported a larger unspecific bacterial load in or on the epithelial cells taken from a diseased site compared to a healthy sulcus. The current available data is of low to moderate quality and inconsistent mainly due to study design, poor reporting and methodological diversity. As so, there is insufficient evidence to support or exclude the invasion by periodontal pathogens as a key step in the

  3. The use of ozonated water and 0.2% chlorhexidine in the treatment of periodontitis patients: A clinical and microbiologic study

    Directory of Open Access Journals (Sweden)

    Kshitish Durga

    2010-01-01

    Full Text Available Background: The development of periodontal disease has been thought to be associated with several restricted members of the oral anaerobic species, such as black-pigmented Porphyromonas species and Actinobacillus actinomycetemcomitans (Aa, in the subgingival environment. Apart from bacteria, certain viruses and fungi that are associated with periodontal disease are also present in the subgingival plaque . Materials and Methods: A randomized, double-blind, crossover split-mouth design was performed. A total of 16 patients suffering from generalized chronic periodontitis were selected for the study. The study period of 18 days was divided into two time-intervals, i.e. baseline (0 days to 7 th day, with a washout period of 4 days followed by a second time interval of 7 days. The use of ozone and chlorhexidine gluconate (CHX irrigation was randomized. Both the patient and the clinician evaluating the clinical parameters were blinded regarding the type of irrigation used. Results: The interpretation of clinical and microbial data is from baseline to 7 th day. A higher percentage of plaque index (12%, gingival index (29% and bleeding index (26% reduction was observed using ozone irrigation as compared to chlorhexidine. The percentile reduction of Aa (25% using ozone was appreciable as compared to no change in Aa occurrence using chlorhexidine. By using O 3 and chlorhexidine, there was no antibacterial effect on Porphyromonas gingivalis (Pg and Tannerella forsythensis. The antifungal effect of ozone from baseline (37% to 7 th day (12.5% was pronounced during the study period, unlike CHX, which did not demonstrate any antifungal effect. Conclusion: Ozone may be considered as an alternative management strategy due to its powerful ability to inactivate microorganisms. Also, there is growing evidence that ozone can be employed as a useful therapeutic agent in both dentistry and medicine.

  4. Targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time PCR.

    Science.gov (United States)

    Price, R R; Viscount, H B; Stanley, M C; Leung, K-P

    2007-01-01

    An in vitro plaque model based on the use of human salivary bacteria and tooth-like surfaces was previously developed for studying the formation of oral biofilm and its use for pre-clinical testing of candidate antimicrobial or antiplaque agents. In this study, a quantitative Taqman PCR assay (QPCR) was developed to compare the bacterial compositions of in vitro biofilms to parent saliva samples, and to determine the relative contributions of different species in the formation of the oral biofilm. In addition, the growth inhibition of saliva-derived plaque was evaluated by chlorhexidine. With this assay, which consisted of primer/probe sets targeting either 16S rDNA sequences present in public databases or cloned ribosomal intergenic spacer region (ISR) sequences, 15 oral bacteria derived from saliva as well as those that were responsible for biofilm formation in an in vitro plaque model were rapidly identified and quantified. Among the target organisms were Actinobacillus actinomycetemcomitans, Eikenella corrodens, Fusobacterium nucleatum, Lactobacillus acidophilus, Micromonas micros, Porphyromonas gingivalis, Prevotella intermedia, Streptococcus mutans, Streptococcus sobrinus, Tannerella forsythensis, and Veillonella parvula. Primer and probe sets developed were both sensitive and specific. The relative profiles of a number of bacteria in 45-h-old biofilms were determined and, when compared to saliva samples, it was found that most of the bacteria identified in saliva also populated the in vitro plaque, including some anaerobes. Brief exposure of biofilms to chlorhexidine resulted in significant losses in viability. This new broad spectrum QPCR assay in combination with the in vitro plaque model will be of significant value in the quantitative study of the microbial composition of human saliva, saliva-derived plaque, and pre-clinical evaluation of potential antimicrobial and antiplaque molecules.

  5. Quantitative molecular detection of putative periodontal pathogens in clinically healthy and periodontally diseased subjects.

    Directory of Open Access Journals (Sweden)

    André Göhler

    Full Text Available Periodontitis is a multi-microbial oral infection with high prevalence among adults. Putative oral pathogens are commonly found in periodontally diseased individuals. However, these organisms can be also detected in the oral cavity of healthy subjects. This leads to the hypothesis, that alterations in the proportion of these organisms relative to the total amount of oral microorganisms, namely their abundance, rather than their simple presence might be important in the transition from health to disease. Therefore, we developed a quantitative molecular method to determine the abundance of various oral microorganisms and the portion of bacterial and archaeal nucleic acid relative to the total nucleic acid extracted from individual samples. We applied quantitative real-time PCRs targeting single-copy genes of periodontal bacteria and 16S-rRNA genes of Bacteria and Archaea. Testing tongue scrapings of 88 matched pairs of periodontally diseased and healthy subjects revealed a significantly higher abundance of P. gingivalis and a higher total bacterial abundance in diseased subjects. In fully adjusted models the risk of being periodontally diseased was significantly higher in subjects with high P. gingivalis and total bacterial abundance. Interestingly, we found that moderate abundances of A. actinomycetemcomitans were associated with reduced risk for periodontal disease compared to subjects with low abundances, whereas for high abundances, this protective effect leveled off. Moderate archaeal abundances were health associated compared to subjects with low abundances. In conclusion, our methodological approach unraveled associations of the oral flora with periodontal disease, which would have gone undetected if only qualitative data had been determined.

  6. Putative periodontopathogens in "diseased" and "non-diseased" persons exhibiting poor oral hygiene.

    Science.gov (United States)

    Dahlén, G; Manji, F; Baelum, V; Fejerskov, O

    1992-01-01

    The aim of the study was to assess the occurrence of some putative periodonto-pathogens in "test" and "control" sites in "diseased" and "non-diseased" persons, respectively, from an adult rural Kenyan population exhibiting poor oral hygiene and widespread loss of attachment (LA). 14 persons (less than 35 years) were assigned to a "diseased" category on the basis of at least 4 sites with LA greater than or equal to 4 mm; at least 5 mm LA and a pocket greater than or equal to 4 mm interproximally in a lower incisor ("test" site): and less than 2 mm LA and no pocket greater than or equal to 4 mm distal to a lower canine or mesial to a lower first premolar ("control" site). Age-matched "non-diseased" persons were identified on the basis of no sites with LA greater than 2 mm and no pockets greater than or equal to 4 mm associated with LA. Paperpoint samples from test and control sites as well as a scraping sample from the dorsum of tongue were examined for presence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Bacteroides intermedius, B. melaninogenicus group, Capnocytophaga, Selenomonas spp., and Wolinella recta. P. gingivalis was found in 79% of test sites and 36% of control sites in "diseased" persons, and in 18% and 35% of test and control sites, respectively, in "non-diseased" persons. "No other bacterial group discriminated significantly between test and control sites or between diseased and non-diseased subjects. The surprisingly high occurrence of P. gingivalis in non-diseased subjects, both subgingivally and on tongue, indicates that deep periodontal pockets are not prerequisite ecological environments for P. gingivalis establishment.

  7. Microbiología pulpar de dientes íntegros con lesiones apicales de origen idiopático

    Directory of Open Access Journals (Sweden)

    Patricia Rodríguez

    2008-03-01

    Full Text Available Introducción: Los cambios periapicales denominados lesiones, en dientes con integridad coronal completa y sin antecedentes de trauma, no presentan una etiología clara. Objetivo: Determinar la presencia de microorganismos en el tejido pulpar clarifica las causas de su muerte y el consiguiente daño a los tejidos periodontalesMateriales y métodos: Se seleccionaron 23 dientes, en personas con rangos de edad entre 10 y 39 años. Las muestras se tomaron con puntas de papel y limas Nº 0.8 (estériles, se transportaron en VMGA III, se procesaron en las siguientes 24 horas de tomada la muestra y se sembraron en agar brucella. Resultados: Los dientes más afectados fueron los centrales superiores 43.8%. De los 23 dientes estudiados, en 20 se observó crecimiento microbiológico. Se identificaron los siguientes microorganismos: Fusobacterium spp., 25%; Eubacte-rium spp., 15%; Peptostreptococcus spp., 10%; Campylobacter spp., 10%; bacilos entéricos gram negativos, 10%; Porphyromonas gingivalis, 10%; Prevotella intermedia, 5%; Eikenellia corrodens, 5%; Dialister pneumosintes, 5%; y levaduras en 5%. No hubo evidencias de crecimiento de Actinomyces actinomycetemcomitans, Tanerella forsythensis ni de estreptococo b hemolítico.Discusión y conclusiones: El tejido pulpar sano es estéril, la lesión sobre él causa inflamación degeneración, muerte pulpar y contaminación bacteriana. Los resultados en el presente estudio determinaron claramente la presencia de micro-organismos en lesiones apicales cerradas de origen endodóntico. De igual forma se evidencia que gran parte de los microorganismos que se encontraron son considerados periodontopatógenos lo que puede igualmente sugerir manejo compartido entre tratamiento endodóntico, periodontal y farmacológico.

  8. Research progress on dispersin B%分散蛋白B的研究进展

    Institute of Scientific and Technical Information of China (English)

    陈珊珊; 齐霞; 赵蕾

    2013-01-01

      细菌自身合成的细胞外多糖是生物膜抵抗抗菌物质和宿主免疫成分的重要保护屏障,因此降解细胞外多糖或抑制其合成是目前研发抗生物膜药物的热点之一。分散蛋白 B 是近年来在牙周可疑致病菌伴放线嗜血杆菌生物膜中发现的一种β-己糖苷酶,具有降解细胞外多糖的作用,是一种潜在的临床抗微生物膜感染类药物。本文就分散蛋白 B 及其结构和作用机制以及应用等研究进展作一综述。%Extracellular polysaccharide, synthesized by the bacteria in the biofilm, is the vital barrier which pro-tect the biofilm against antimicrobial agents and host immune components. Therefore, researches focus on the anti-biolfilm agents which exhibit the capability to degrade the extracellular polysaccharide or inhibit its synthesis are of high interest. Dispersin B, a β-N-acetylglucosaminidase produced by the gram-negative periodontal pathogen Actinobacillus actinomycetemcomitans, is reported to show the effect to degrade extracellular polysaccharide recent-ly, and it may be possess the potential of using as an anti-biolfilm agents in clinic. This review will summarize the recent research progress on the structure, mechanism of action and application of dispersin B.

  9. Changes in subgingival microflora after placement and removal of fixed orthodontic appliances

    Directory of Open Access Journals (Sweden)

    Živković-Sandić Marija

    2014-01-01

    Full Text Available Introduction. The placement of fixed orthodontic appliances may lead to increased plaque accumulation and changes in subgingival microflora. Objective. The aim of this study was to examine the changes in frequency of subgingival microflora that occur after placement and removal of fixed orthodontic appliance using polymerase chain reaction (PCR. Methods. This study included 33 orthodontic patients, who were divided into two groups. Subgingival plaque samples were collected from the right upper incisor (U1 and right upper first molar (U6. In group A, the samples were taken three times: before placement appliance (T1, after one month (T2, and after 3 months (T3. In group B the samples were also taken three times: before appliance removal (T1, after one month (T2, and after three months (T3. PCR method was used to determine the presence of P. gingivalis, A. actinomycetemcomitans, T. forsythia, and P. intermedia. Results. In group A the frequency of P. gingivalis showed statistically significant decrease at U1 (p=0.049 and U6 (p=0.008, from T1 to T2, and at U1 (p=0.048 from T1 to T3. In group B only the frequency of T. forsythia showed a statistically significant decrease, at U6 (T1 vs. T2, p=0.004; T1 vs. T3, p=0.0003. Regarding other analyzed bacteria, changes in the presence were noticed but no statistical significance was found. Conclusion. Placement of fixed appliances may have an impact on subgingival microflora, but in the first months after the placement and removal of the appliance changes were not significant, probably due to good oral hygiene. [Projekat Ministarstva nauke Republike Srbije, br. 175075

  10. 二次方程ax4+bx2y2+dy4=cz2的可解性%ON THE SOLVABILITY OF THE QUARTIC EQUATION ax4+ bx2y2 + dy4= cz2

    Institute of Scientific and Technical Information of China (English)

    袁平之; 林军

    2000-01-01

    The authors generalize Euler's famous criterion to the Diophantine equation of the title. By using this criterion, the authors obtain a simple criterion containing Zhehg's complicated one on the equation x4 + k x2y2 + y4 = z2%作者推广了题述不定方程的著名的Euler判别准则,应用该准则,得到了关于不定方程x4+kx2y2+y4=z2的一个简单的判别准则,它简化了郑德勋1989年给出的结果.

  11. Estación de bombeo de agua autónoma eólico-fotovoltaica 2,4MWh/mes en Sarracín (Burgos)

    OpenAIRE

    Carranza Barcenilla, Víctor

    2013-01-01

    Se desea obtener mediante el uso de energías renovables la autonomía energética de la estación de bombeo de agua de Sarracín (Burgos) El objetivo es tener dos depósitos de agua llenos, para en caso de rotura o fallo del suministro eléctrico conectado a red no desabastecer a la población. La instalación planteada cuenta con un sistema de captación de energía compuesto por 30 módulos fotovoltaicos y 4 aerogeneradores, un sistema de regulación formado por 2 reguladores solares y 4 eólic...

  12. Existence and uniqueness of solutions of nonlinear two-point boundary value problems for fourth order differential equations%四阶微分方程非线性两点边值问题解的存在唯一性

    Institute of Scientific and Technical Information of China (English)

    高永馨; 谢燕华

    2012-01-01

    利用上下解方法,讨论了四阶微分方程非线性两点边值问题y(4) =f(x,y,y′,y″,y(′″)),y(b) =b0,y′(b) =b1,y″(b) =h(y″(a)),g(y(a),y(b),y′(a),y′(b),y″(a),y″(b),y(′″)(a),y(′″)(b)) =0解的存在唯一性.%By using the method of upper - lower solution,the existence and uniquenss of solutions of nonlinear two -point boundary value problems for fourth order differential equation y(4) =f(x,y,y′,y″,y(′″)),y(b) =b0,y′(b) =b1,y″(b) =h(y″(a)),g(y(a),y(b),y′(a),y′(b),y″(a),y″(b),y(′″)(a),y(′″)(b)) =0 are investigated.

  13. Measurement of the B-Meson Inclusive Semileptonic Branching Fraction and Electron-Energy Moments

    CERN Document Server

    Mahmood, A H; Bonvicini, G; Cinabro, D; Dubrovin, M; Bornheim, A; Lipeles, E; Pappas, S P; Shapiro, A; Weinstein, A J; Briere, R A; Chen, G P; Ferguson, T; Tatishvili, G T; Vogel, H; Watkins, M E; Adam, N E; Alexander, J P; Berkelman, K; Boisvert, V; Cassel, D G; Duboscq, J E; Ecklund, K M; Ehrlich, R; Galik, R S; Gibbons, L; Gittelman, B; Gray, S W; Hartill, D L; Heltsley, B K; Hsu, L; Jones, C D; Kandaswamy, J; Kreinick, D L; Kuznetsov, V E; Magerkurth, A; Mahlke-Krüger, H; Meyer, T O; Patterson, J R; Pedlar, T K; Peterson, D; Pivarski, J; Riley, D; Sadoff, A J; Schwarthoff, H; Shepherd, M R; Sun, W M; Thayer, J G; Urner, D; Wilksen, T; Weinberger, M; Athar, S B; Avery, P; Breva-Newell, L; Potlia, V; Stöck, H; Yelton, J; Eisenstein, B I; Gollin, G D; Karliner, I; Lowrey, N; Naik, P; Sedlack, C; Selen, M; Thaler, J J; Williams, J; Edwards, K W; Besson, D; Gao, K Y; Gong, D T; Kubota, Y; Li, S Z; Poling, R A; Scott, A W; Smith, A; Stepaniak, C J; Urheim, J; Metreveli, Z V; Seth, K K; Tomaradze, A G; Zweber, P; Ernst, J; Arms, K; Eckhart, E; Gan, K K; Gwon, C; Severini, H; Skubic, P L; Asner, D M; Dytman, S A; Mehrabyan, S S; Müller, J A; Nam, S; Savinov, V; Huang, G S; Miller, D H; Pavlunin, V; Sanghi, B; Shibata, E I; Shipsey, I P J; Adams, G S; Chasse, M; Cummings, J P; Danko, I; Napolitano, J; Cronin-Hennessy, D; Park, C S; Park, W; Thayer, J B; Thorndike, E H; Coan, T E; Gao, Y S; Liu, F; Stroynowski, R; Artuso, M; Boulahouache, C; Blusk, S; Butt, J; Dambasuren, E; Dorjkhaidav, O; Haynes, J; Menaa, N; Mountain, R; Muramatsu, H; Nandakumar, R; Redjimi, R; Sia, R; Skwarnicki, T; Stone, S; Wang, J C; Zhang, K

    2004-01-01

    We report a new measurement of the B-meson semileptonic decay momentum spectrum that has been made with a sample of 9.4/fb of electron-positron annihilation data collected with the CLEO II detector at the Y(4S) resonance. Electrons from primary semileptonic decays and secondary charm decays were separated by using charge and angular correlations in Y(4S) events with a high-momentum lepton and an additional electron. We determined the semileptonic branching fraction to be (10.91 +- 0.09 +- 0.24)% from the normalization of the electron-energy spectrum. We also measured the moments of the electron energy spectrum with minimum energies from 0.6 GeV to 1.5 GeV.

  14. Fauna y bioestratigrafía del yacimiento Aragoniense de Montejo de la Vega de la Serrezuela (Segovia

    Directory of Open Access Journals (Sweden)

    Armenteros, I.

    1998-12-01

    Full Text Available The macro and micromammals fossils of Montejo de la Vega de la Serrezuela (Segovia, Spain are studied. The identified taxons allow to place the new locality in the Middle Aragonian, unit MN5 of Mein (1977, 1979, 1990. The Montejo de la Vega deposit can be correlated with the Arroyo del Olivar in the Madrid basin and the Las Planas 4A, 4B, Y4C in the Calatayud-Teruel basin.Se estudian los macro y micromamíferos fósiles de Montejo de la Vega de la Serrezuela (Segovia. Los taxones identificados permiten situar esta localidad en el Aragoniense medio, unidad MN5 de Mein (1977, 1979, 1990. El yacimiento de Montejo de la Vega puede correlacionarse con el de Arroyo del Olivar en la cuenca de Madrid y con los de Las Planas 4A, 4B y 4C en la cuenca de Calatayud-Teruel.

  15. Osteocytes serve as a progenitor cell of osteosarcoma.

    Science.gov (United States)

    Sottnik, Joseph L; Campbell, Brittany; Mehra, Rohit; Behbahani-Nejad, Omid; Hall, Christopher L; Keller, Evan T

    2014-08-01

    Osteosarcoma (OSA) is the most common primary bone tumor in humans. However, the cell of origin of OSA is not clearly defined although there is evidence that osteoblasts may serve as OSA progenitors. The role of osteocytes, terminally differentiated osteoblasts, as OSA progenitors has yet to be described. Analysis of patient cDNA from publicly available microarray data revealed that patients with OSA have increased expression of dentin matrix phosphoprotein 1 (DMP1), a marker of osteocytes. Analysis of multiple murine, human, and canine OSA cell lines revealed DMP1 expression. To test the tumorigenic potential of osteocytes, MLO-Y4, a SV-40 immortalized murine osteocyte cell line, was injected into subcutaneous and orthotopic (intratibial) sites of mice. Tumor growth occurred in both locations. Orthotopic MLO-Y4 tumors produced mixed osteoblastic/osteolytic radiographic lesions; a hallmark of OSA. Together, these data demonstrate for the first time that osteocytes can serve as OSA progenitors. PMID:24700678

  16. Adsorption of and acidic dye from aqueous solution by surfactant modified bentonite

    International Nuclear Information System (INIS)

    The aim of this paper is to study the adsorption of an acidic dye S. Y. 4 GL (i.e: Supranol yellow 4GL) from aqueous solution on inorgano-organo clay. Bentonite is a kind of natural clay with good exchanging ability. By exchanging its inter lamellar cations with Cetyltrimethylammonium bromide (CTAB) and hydroxy aluminic or chromium poly cations, the properties of natural bentonite can be greatly improved. (Author)

  17. Measurement of the (eta c)(1S) production cross-section in proton-proton collisions via the decay (eta c)(1S) -> p(p)over-bar

    NARCIS (Netherlands)

    Aaij, R.; Beteta, C. Abellaen; Adeva, B.; Adinolfi, M.; Affolder, A.; Ajaltouni, Z.; Akar, S.; Albrecht, J.; Alessio, F.; Alexander, M.; Ali, S.; Alkhazov, G.; Alvarez Cartelle, P.; Alves, A. A.; Amato, S.; Amerio, S.; Amhis, Y.; An, L.; Anderlini, L.; Andreassen, R.; Andreotti, M.; Andrews, J. E.; Appleby, R. B.; Gutierrez, O. Aquines; Archilli, F.; Artamonov, A.; Artuso, M.; Aslanides, E.; Auriemma, G.; Baalouch, M.; Bachmann, S.; Back, J. J.; Badalov, A.; Baesso, C.; Baldini, W.; Barlow, R. J.; Barschel, C.; Barsuk, S.; Barter, W.; Batozskaya, V.; Battista, V.; Beaucourt, L.; Beddow, J.; Bedeschi, F.; Bediaga, I.; Belogurov, S.; Belous, K.; Belyaev, I.; Ben-Haim, E.; Bencivenni, G.; Benson, S.; Benton, J.; Berezhnoy, A.; Bernet, R.; Bettler, M. -O.; van Beuzekom, M.; Bien, A.; Bifani, S.; Bird, T.; Bizzeti, A.; Bjornstad, P. M.; Blake, T.; Blanc, F.; Blouw, J.; Blusk, S.; Bocci, V.; Bondar, A.; Bondar, N.; Bonivento, W.; Borghi, S.; Borgia, A.; Borsato, M.; Bowcock, T. J. V.; Bowen, E.; Bozzi, C.; Brambach, T.; Bressieux, J.; Brett, D.; Britsch, M.; Britton, T.; Brodzicka, J.; Brook, N. H.; Brown, H.; Bursche, A.; Busetto, G.; Buytaert, J.; Cadeddu, S.; Calabrese, R.; Calvi, M.; Calvo Gomez, M.; Campana, P.; Perez, D. Campora; Carbone, A.; Carboni, G.; Cardinale, R.; Cardini, A.; Carson, L.; Carvalho Akiba, K.; Casse, G.; Cassina, L.; Garcia, L. Castillo; Cattaneo, M.; Cauet, Ch.; Cenci, R.; Charles, M.; Charpentier, Ph.; Chefdeville, M.; Cheung, S. -F.; Chiapolini, N.; Chrzaszcz, M.; Ciba, K.; Vidal, X. Cid; Ciezarek, G.; Clarke, P. E. L.; Clemencic, M.; Cliff, H. V.; Closier, J.; Coco, V.; Cogan, J.; Cogneras, E.; Cogoni, V.; Cojocariu, L.; Collins, P.; Comerma-Montells, A.; Contu, A.; Cook, A.; Coombes, M.; Coquereau, S.; Corti, G.; Corvo, M.; Counts, I.; Couturier, B.; Cowan, G. A.; Craik, D. C.; Torres, M. Cruz; Cunliffe, S.; Currie, R.; D'Ambrosio, C.; Dalseno, J.; David, P.; David, P. N. Y.; Davis, A.; De Bruyn, K.; De Capua, S.; De Cian, M.; De Miranda, J. M.; De Paula, L.; De Silva, W.; De Simone, P.; Decamp, D.; Deckenhoff, M.; Del Buono, L.; Deleage, N.; Derkach, D.; Deschamps, O.; Dettori, F.; Di Canto, A.; Donleavy, S.; Dordei, F.; Dorigo, M.; Dosil Suarez, A.; Dossett, D.; Dovbnya, A.; Dreimanis, K.; Dujany, G.; Dupertuis, F.; Durante, P.; Dzhelyadin, R.; Dziurda, A.; Dzyuba, A.; Easo, S.; Egede, U.; Egorychev, V.; Eidelman, S.; Eisenhardt, S.; Eitschberger, U.; Ekelhof, R.; Eklund, L.; El Rifai, I.; Elena, E.; Elsasser, Ch.; Ely, S.; Esen, S.; Evans, H. -M.; Evans, T.; Falabella, A.; Farber, C.; Farinelli, C.; Farley, N.; Farry, S.; Fay, R. F.; Ferguson, D.; Fernandez Albor, V.; Ferreira Rodrigues, F.; Ferro-Luzzi, M.; Filippov, S.; Fiore, M.; Fiorini, M.; Firlej, M.; Fitzpatrick, C.; Fiutowski, T.; Fol, P.; Fontana, M.; Fontanelli, F.; Forty, R.; Francisco, O.; Frei, C.; Frosini, M.; Furfaro, E.; Gallas Torreira, A.; Galli, D.; Gallorini, S.; Gambetta, S.; Gandelman, M.; Gandini, P.; Garcia Pardinas, J.; Garofoli, J.; Garra Tico, J.; Garrido, L.; Gaspar, C.; Gauld, R.; Gavardi, L.; Gavrilov, G.; Geraci, A.; Gersabeck, E.; Gersabeck, M.; Gershon, T.; Ghez, Ph.; Gianelle, A.; Giani, S.; Gibson, V.; Giubega, L.; Gligorov, V. V.; Gobel, C.; Golubkov, D.; Golutvin, A.; Gotti, C.; Grabalosa Gaendara, M.; Graciani Diaz, R.; Granado Cardoso, L. A.; Grauges, E.; Graziani, G.; Grecu, A.; Greening, E.; Gregson, S.; Griffith, P.; Grillo, L.; Gruenberg, O.; Gui, B.; Gushchin, E.; Guz, Yu.; Gys, T.; Hadjivasiliou, C.; Haefeli, G.; Haen, C.; Haines, S. C.; Hall, S.; Hamilton, B.; Hampson, T.; Han, X.; Hansmann-Menzemer, S.; Harnew, N.; Harnew, S. T.; Harrison, J.; He, J.; Head, T.; Heijne, V.; Hennessy, K.; Henrard, P.; Henry, L.; Hernando Morata, J. A.; van Herwijnen, E.; Hess, M.; Hicheur, A.; Hill, D.; Hoballah, M.; Hombach, C.; Hulsbergen, W.; Hunt, P.; Hussain, N.; Hutchcroft, D.; Hynds, D.; Idzik, M.; Ilten, P.; Jacobsson, R.; Jalocha, J.; Jans, E.; Jaton, P.; Jawahery, A.; Jing, F.; John, M.; Johnson, D.; Jones, C. R.; Joram, C.; Jost, B.; Jurik, N.; Kaballo, M.; Kandybei, S.; Kanso, W.; Karacson, M.; Karbach, T. M.; Karodia, S.; Kelsey, M.; Kenyon, I. R.; Ketel, T.; Khanji, B.; Khurewathanakul, C.; Klaver, S.; Klimaszewski, K.; Kochebina, O.; Kolpin, M.; Komarov, I.; Koppenburg, P.; Korolev, M.; Kozlinskiy, A.; Kravchuk, L.; Kreplin, K.; Kreps, M.; Krocker, G.; Krokovny, P.; Kucewicz, W.; Kucharczyk, M.; Kudryavtsev, V.; Kurek, K.; Kvaratskheliya, T.; La Thi, V. N.; Lacarrere, D.; Lafferty, G.; Lai, A.; Lambert, D.; Lambert, R. W.; Lanfranchi, G.; Langenbruch, C.; Langhans, B.; Latham, T.; Lazzeroni, C.; Le Gac, R.; van Leerdam, J.; Lees, J. -P.; Lefevre, R.; Leflat, A.; Lefrancois, J.; Leo, S.; Leroy, O.; Lesiak, T.; Leverington, B.; Likhomanenko, T.; Liles, M.; Lindner, R.; Linn, C.

    2015-01-01

    The production of the eta(c)(1S) state in protonproton collisions is probed via its decay to the p (p) over bar final state with the LHCb detector, in the rapidity range 2.0 <y <4.5 and in the meson transverse-momentum range p(T) > 6.5GeV/c. The cross-section for prompt production of eta(c)(1S) meso

  18. Cada cuánto me tengo que hacer la prueba de Papanicoláu (How Often to Get a Pap Test)

    Centers for Disease Control (CDC) Podcasts

    2013-01-10

    El cáncer de cuello uterino ha disminuido en los Estados Unidos, pero cada año se le diagnostica a más de 12,000 mujeres y 4,000 mueren. Este podcast habla sobre la importancia de las pruebas de Papanicoláu.  Created: 1/10/2013 by MMWR.   Date Released: 11/4/2013.

  19. Calcium signaling and the novel anti-proliferative effect of the UTP-sensitive P2Y11 receptor in rat cardiac myofibroblasts.

    Science.gov (United States)

    Certal, Mariana; Vinhas, Adriana; Pinheiro, Ana Rita; Ferreirinha, Fátima; Barros-Barbosa, Aurora Raquel; Silva, Isabel; Costa, Maria Adelina; Correia-de-Sá, Paulo

    2015-11-01

    During myocardial ischemia and reperfusion both purines and pyrimidines are released into the extracellular milieu, thus creating a signaling wave that propagates to neighboring cells via membrane-bound P2 purinoceptors activation. Cardiac fibroblasts (CF) are important players in heart remodeling, electrophysiological changes and hemodynamic alterations following myocardial infarction. Here, we investigated the role UTP on calcium signaling and proliferation of CF cultured from ventricles of adult rats. Co-expression of discoidin domain receptor 2 and α-smooth muscle actin indicate that cultured CF are activated myofibroblasts. Intracellular calcium ([Ca(2+)]i) signals were monitored in cells loaded with Fluo-4 NW. CF proliferation was evaluated by the MTT assay. UTP and the selective P2Y4 agonist, MRS4062, caused a fast desensitizing [Ca(2+)]i rise originated from thapsigargin-sensitive internal stores, which partially declined to a plateau providing the existence of Ca(2+) in the extracellular fluid. The biphasic [Ca(2+)]i response to UTP was attenuated respectively by P2Y4 blockers, like reactive blue-2 and suramin, and by the P2Y11 antagonist, NF340. UTP and the P2Y2 receptor agonist MRS2768 increased, whereas the selective P2Y11 agonist NF546 decreased, CF growth; MRS4062 was ineffective. Blockage of the P2Y11 receptor or its coupling to adenylate cyclase boosted UTP-induced CF proliferation. Confocal microscopy and Western blot analysis confirmed the presence of P2Y2, P2Y4 and P2Y11 receptors. Data indicate that besides P2Y4 and P2Y2 receptors which are responsible for UTP-induced [Ca(2+)]i transients and growth of CF, respectively, synchronous activation of the previously unrecognized P2Y11 receptor may represent an important target for anti-fibrotic intervention in cardiac remodeling.

  20. Measurement of alpha/phi2 in Bo --> pi pi, rho pi and rho rho

    CERN Document Server

    Dalseno, J

    2011-01-01

    We present a summary of the measurements of the CKM angle, alpha (phi2), performed by the BaBar and Belle experiments which collect BBbar pairs at the Y(4S) resonance produced in asymmetric e+ e- collisions. We discuss the measurements of the branching fractions and CP asymmetries in the B --> pi pi, rho pi and rho rho final states that lead to constraints on alpha (phi2).

  1. Suppression of ϒ{hooked}(1S) at forward rapidity in Pb-Pb collisions at sNN=2.76 TeV

    NARCIS (Netherlands)

    Abelev, B.; Adam, J.; Adamová, D.; Aggarwal, M. M.; Agnello, M.; Agostinelli, A.; Agrawal, N.; Ahammed, Z.; Ahmad, N.; Ahmed, I.; Ahn, S. U.; Ahn, S. A.; Aimo, I.; Aiola, S.; Ajaz, M.; Akindinov, A.; Alam, S. N.; Aleksandrov, D.; Alessandro, B.; Alexandre, D.; Alici, A.; Alkin, A.; Alme, J.; Alt, T.; Altinpinar, S.; Altsybeev, I.; Alves Garcia Prado, C.; Andrei, C.; Andronic, A.; Anguelov, V.; Anielski, J.; Antičić, T.; Antinori, F.; Antonioli, P.; Aphecetche, L.; Appelshäuser, H.; Arcelli, S.; Armesto, N.; Arnaldi, R.; Aronsson, T.; Arsene, I. C.; Arslandok, M.; Augustinus, A.; Averbeck, R.; Awes, T. C.; Azmi, M. D.; Bach, M.; Badalà, A.; Baek, Y. W.; Bagnasco, S.; Bailhache, R.; Bala, R.; Baldisseri, A.; Baltasar Dos Santos Pedrosa, F.; Baral, R. C.; Barbera, R.; Barile, F.; Barnaföldi, G. G.; Barnby, L. S.; Barret, V.; Bartke, J.; Basile, M.; Bastid, N.; Basu, S.; Bathen, B.; Batigne, G.; Batyunya, B.; Batzing, P. C.; Baumann, C.; Bearden, I. G.; Beck, H.; Bedda, C.; Behera, N. K.; Belikov, I.; Bellwied, R.; Belmont-Moreno, E.; Belmont, R.; Belyaev, V.; Bencedi, G.; Beole, S.; Berceanu, I.; Bercuci, A.; Berdnikov, Y.; Berenyi, D.; Berger, M. E.; Bertens, R. A.; Berzano, D.; Betev, L.; Bhasin, A.; Bhati, A. K.; Bhattacharjee, B.; Bhom, J.; Bianchi, L.; Bianchi, N.; Bianchin, C.; Bielčík, J.; Bielčíková, J.; Bilandzic, A.; Bjelogrlic, S.; Blanco, F.; Blau, D.; Blume, C.; Bock, F.; Bogdanov, A.; Bøggild, H.; Bogolyubsky, M.; Böhmer, F. V.; Boldizsár, L.; Bombara, M.; Book, J.; Borel, H.; Borissov, A.; Bossú, F.; Botje, M.; Botta, E.; Böttger, S.; Braun-Munzinger, P.; Bregant, M.; Breitner, T.; Broker, T. A.; Browning, T. A.; Broz, M.; Bruna, E.; Bruno, G. E.; Budnikov, D.; Buesching, H.; Bufalino, S.; Buncic, P.; Busch, O.; Buthelezi, Z.; Caffarri, D.; Cai, X.; Caines, H.; Calero Diaz, L.; Caliva, A.; Calvo Villar, E.; Camerini, P.; Carena, F.; Carena, W.; Castillo Castellanos, J.; Casula, E. A R; Catanescu, V.; Cavicchioli, C.; Ceballos Sanchez, C.; Cepila, J.; Cerello, P.; Chang, B.; Chapeland, S.; Charvet, J. L.; Chattopadhyay, S.; Chattopadhyay, S.; Cherney, M.; Cheshkov, C.; Cheynis, B.; Chibante Barroso, V.; Chinellato, D. D.; Chochula, P.; Chojnacki, M.; Choudhury, S.; Christakoglou, P.; Christensen, C. H.; Christiansen, P.; Chujo, T.; Chung, S. U.; Cicalo, C.; Cifarelli, L.; Cindolo, F.; Cleymans, J.; Colamaria, F.; Colella, D.; Collu, A.; Colocci, M.; Conesa Balbastre, G.; Conesa del Valle, Z.; Connors, M. E.; Contreras, J. G.; Cormier, T. M.; Corrales Morales, Y.; Cortese, P.; Cortés Maldonado, I.; Cosentino, M. R.; Costa, F.; Crochet, P.; Cruz Albino, R.; Cuautle, E.; Cunqueiro, L.; Dainese, A.; Dang, R.; Das, D.; Das, I.; Das, K.; Das, S.; Dash, A.; Dash, S.; De, S.; Delagrange, H.; Deloff, A.; Dénes, E.; D'Erasmo, G.; De Caro, A.; de Cataldo, G.; de Cuveland, J.; De Falco, A.; De Gruttola, D.; De Marco, N.; De Pasquale, S.; de Rooij, R.; Diaz Corchero, M. A.; Dietel, T.; Divià, R.; Di Bari, D.; Di Liberto, S.; Di Mauro, A.; Di Nezza, P.; Djuvsland, O.; Dobrin, A.; Dobrowolski, T.; Domenicis Gimenez, D.; Dönigus, B.; Dordic, O.; Dørheim, S.; Dubey, A. K.; Dubla, A.; Ducroux, L.; Dupieux, P.; Dutta Majumdar, A. K.; Ehlers, R. J.; Elia, D.; Engel, H.; Erazmus, B.; Erdal, H. A.; Eschweiler, D.; Espagnon, B.; Esposito, M.; Estienne, M.; Esumi, S.; Evans, D.; Evdokimov, S.; Fabris, D.; Faivre, J.; Falchieri, D.; Fantoni, A.; Fasel, M.; Fehlker, D.; Feldkamp, L.; Felea, D.; Feliciello, A.; Feofilov, G.; Ferencei, J.; Fernández Téllez, A.; Ferreiro, E. G.; Ferretti, A.; Festanti, A.; Figiel, J.; Filchagin, S.; Finogeev, D.; Fionda, F. M.; Fiore, E. M.; Floratos, E.; Floris, M.; Foertsch, S.; Foka, P.; Fokin, S.; Fragiacomo, E.; Francescon, A.; Frankenfeld, U.; Fuchs, U.; Furget, C.; Fusco Girard, M.; Gaardhøje, J. J.; Gagliardi, M.; Gago, A. M.; Gallio, M.; Gangadharan, D. R.; Ganoti, P.; Garabatos, C.; Garcia-Solis, E.; Gargiulo, C.; Garishvili, I.; Gerhard, J.; Germain, M.; Gheata, A.; Gheata, M.; Ghidini, B.; Ghosh, P.; Ghosh, S. K.; Gianotti, P.; Giubellino, P.; Gladysz-Dziadus, E.; Glässel, P.; Gomez Ramirez, A.; González-Zamora, P.; Gorbunov, S.; Görlich, L.; Gotovac, S.; Graczykowski, L. K.; Grelli, A.; Grigoras, A.; Grigoras, C.; Grigoriev, V.; Grigoryan, A.; Grigoryan, S.; Grinyov, B.; Grion, N.; Grosse-Oetringhaus, J. F.; Grossiord, J. Y.; Grosso, R.; Guber, F.; Guernane, R.; Guerzoni, B.; Guilbaud, M.; Gulbrandsen, K.; Gulkanyan, H.; Gumbo, M.; Gunji, T.; Gupta, A.; Gupta, R.; Khan, K. H.; Haake, R.; Haaland, O.; Hadjidakis, C.; Haiduc, M.; Hamagaki, H.; Hamar, G.; Hanratty, L. D.; Hansen, A.; Harris, J. W.; Hartmann, H.; Harton, A.; Hatzifotiadou, D.; Hayashi, S.; Heckel, S. T.; Heide, M.; Helstrup, H.; Herghelegiu, A.

    2014-01-01

    We report on the measurement of the inclusive ϒ{hooked}(1S) production in Pb-Pb collisions at sNN=2.76 TeV carried out at forward rapidity (2.5<y<4) and down to zero transverse momentum using its μ+μ- decay channel with the ALICE detector at the Large Hadron Collider. A strong suppression of the inc

  2. Exclusive J /ψ photoproduction off protons in ultraperipheral p -Pb collisions at sNN =5.02TeV

    NARCIS (Netherlands)

    Abelev, B.; Adam, J.; Adamová, D.; Aggarwal, M. M.; Aglieri Rinella, G.; Agnello, M.; Agostinelli, A.; Agrawal, N.; Ahammed, Z.; Ahmad, N.; Ahmed, I.; Ahn, S. U.; Ahn, S. A.; Aimo, I.; Aiola, S.; Ajaz, M.; Akindinov, A.; Alam, S. N.; Aleksandrov, D.; Alessandro, B.; Alexandre, D.; Alici, A.; Alkin, A.; Alme, J.; Alt, T.; Altinpinar, S.; Altsybeev, I.; Alves Garcia Prado, C.; Andrei, C.; Andronic, A.; Anguelov, V.; Anielski, J.; Antičić, T.; Antinori, F.; Antonioli, P.; Aphecetche, L.; Appelshäuser, H.; Arcelli, S.; Armesto, N.; Arnaldi, R.; Aronsson, T.; Arsene, I. C.; Arslandok, M.; Augustinus, A.; Averbeck, R.; Awes, T. C.; Azmi, M. D.; Bach, M.; Badalà, A.; Baek, Y. W.; Bagnasco, S.; Bailhache, R.; Bala, R.; Baldisseri, A.; Baltasar Dos Santos Pedrosa, F.; Baral, R. C.; Barbera, R.; Barile, F.; Barnaföldi, G. G.; Barnby, L. S.; Barret, V.; Bartke, J.; Basile, M.; Bastid, N.; Basu, S.; Bathen, B.; Batigne, G.; Batista Camejo, A.; Batyunya, B.; Batzing, P. C.; Baumann, C.; Bearden, I. G.; Beck, H.; Bedda, C.; Behera, N. K.; Belikov, I.; Bellini, F.; Bellwied, R.; Belmont-Moreno, E.; Belmont, R.; Belyaev, V.; Bencedi, G.; Beole, S.; Berceanu, I.; Bercuci, A.; Berdnikov, Y.; Berenyi, D.; Berger, M. E.; Bertens, R. A.; Berzano, D.; Betev, L.; Bhasin, A.; Bhat, I. R.; Bhati, A. K.; Bhattacharjee, B.; Bhom, J.; Bianchi, L.; Bianchi, N.; Bianchin, C.; Bielčík, J.; Bielčíková, J.; Bilandzic, A.; Bjelogrlic, S.; Blanco, F.; Blau, D.; Blume, C.; Bock, F.; Bogdanov, A.; Bøggild, H.; Bogolyubsky, M.; Böhmer, F. V.; Boldizsár, L.; Bombara, M.; Book, J.; Borel, H.; Borissov, A.; Bossú, F.; Botje, M.; Botta, E.; Böttger, S.; Braun-Munzinger, P.; Bregant, M.; Breitner, T.; Broker, T. A.; Browning, T. A.; Broz, M.; Bruna, E.; Bruno, G. E.; Budnikov, D.; Buesching, H.; Bufalino, S.; Buncic, P.; Busch, O.; Buthelezi, Z.; Caffarri, D.; Cai, X.; Caines, H.; Calero Diaz, L.; Caliva, A.; Calvo Villar, E.; Camerini, P.; Carena, F.; Carena, W.; Castillo Castellanos, J.; Casula, E. A R; Catanescu, V.; Cavicchioli, C.; Ceballos Sanchez, C.; Cepila, J.; Cerello, P.; Chang, B.; Chapeland, S.; Charvet, J. L.; Chattopadhyay, S.; Chattopadhyay, S.; Chelnokov, V.; Cherney, M.; Cheshkov, C.; Cheynis, B.; Chibante Barroso, V.; Chinellato, D. D.; Chochula, P.; Chojnacki, M.; Choudhury, S.; Christakoglou, P.; Christensen, C. H.; Christiansen, P.; Chujo, T.; Chung, S. U.; Cicalo, C.; Cifarelli, L.; Cindolo, F.; Cleymans, J.; Colamaria, F.; Colella, D.; Collu, A.; Colocci, M.; Conesa Balbastre, G.; Conesa Del Valle, Z.; Connors, M. E.; Contreras, J. G.; Cormier, T. M.; Corrales Morales, Y.; Cortese, P.; Cortés Maldonado, I.; Cosentino, M. R.; Costa, F.; Crochet, P.; Cruz Albino, R.; Cuautle, E.; Cunqueiro, L.; Dainese, A.; Dang, R.; Danu, A.; Das, D.; Das, I.; Das, K.; Das, S.; Dash, A.; Dash, S.; De, S.; Delagrange, H.; Deloff, A.; Dénes, E.; D'erasmo, G.; De Caro, A.; De Cataldo, G.; De Cuveland, J.; De Falco, A.; De Gruttola, D.; De Marco, N.; De Pasquale, S.; De Rooij, R.; Diaz Corchero, M. A.; Dietel, T.; Dillenseger, P.; Divià, R.; Di Bari, D.; Di Liberto, S.; Di Mauro, A.; Di Nezza, P.; Djuvsland, O.; Dobrin, A.; Dobrowolski, T.; Domenicis Gimenez, D.; Dönigus, B.; Dordic, O.; Dørheim, S.; Dubey, A. K.; Dubla, A.; Ducroux, L.; Dupieux, P.; Dutta Majumdar, A. K.; Hilden, T. E.; Ehlers, R. J.; Elia, D.; Engel, H.; Erazmus, B.; Erdal, H. A.; Eschweiler, D.; Espagnon, B.; Esposito, M.; Estienne, M.; Esumi, S.; Evans, D.; Evdokimov, S.; Fabris, D.; Faivre, J.; Falchieri, D.; Fantoni, A.; Fasel, M.; Fehlker, D.; Feldkamp, L.; Felea, D.; Feliciello, A.; Feofilov, G.; Ferencei, J.; Fernández Téllez, A.; Ferreiro, E. G.; Ferretti, A.; Festanti, A.; Figiel, J.; Figueredo, M. A S; Filchagin, S.; Finogeev, D.; Fionda, F. M.; Fiore, E. M.; Floratos, E.; Floris, M.; Foertsch, S.; Foka, P.; Fokin, S.; Fragiacomo, E.; Francescon, A.; Frankenfeld, U.; Fuchs, U.; Furget, C.; Furs, A.; Fusco Girard, M.; Gaardhøje, J. J.; Gagliardi, M.; Gago, A. M.; Gallio, M.; Gangadharan, D. R.; Ganoti, P.; Gao, C.; Garabatos, C.; Garcia-Solis, E.; Gargiulo, C.; Garishvili, I.; Gerhard, J.; Germain, M.; Gheata, A.; Gheata, M.; Ghidini, B.; Ghosh, P.; Ghosh, S. K.; Gianotti, P.; Giubellino, P.; Gladysz-Dziadus, E.; Glässel, P.; Gomez Ramirez, A.; González-Zamora, P.; Gorbunov, S.; Görlich, L.; Gotovac, S.; Graczykowski, L. K.; Grelli, A.; Grigoras, A.; Grigoras, C.; Grigoriev, V.; Grigoryan, A.; Grigoryan, S.; Grinyov, B.; Grion, N.; Grosse-Oetringhaus, J. F.; Grossiord, J. Y.; Grosso, R.; Guber, F.; Guernane, R.; Guerzoni, B.; Guilbaud, M.; Gulbrandsen, K.; Gulkanyan, H.; Gumbo, M.; Gunji, T.; Gupta, A.; Gupta, R.; Khan, K. H.; Haake, R.; Haaland, O.; Hadjidakis, C.; Haiduc, M.; Hamagaki, H.; Hamar, G.

    2014-01-01

    We present the first measurement at the LHC of exclusive J/ψ photoproduction off protons, in ultraperipheral proton-lead collisions at sNN=5.02TeV. Events are selected with a dimuon pair produced either in the rapidity interval, in the laboratory frame, 2.5<y<4 (p-Pb) or -3.6

  3. An??lisis de la divulgaci??n cient??fica: modelo te??rico y estrategias divulgativas

    OpenAIRE

    Cassany, Daniel

    2003-01-01

    La exposici??n consta de cuatro partes: 1??) justifica el inter??s de una aproximaci??n discursiva a la DC (puntos de partida, tradiciones investigadoras, objetivos y metodolog??a); 2??) resume algunas concepciones sobre la DC y expone el modelo de recontextualizaci??n que proponemos; 3??) comenta ejemplos divulgativos que justifican y matizan el modelo anterior, y 4??) resume los hallazgos m??s importantes y esboza l??neas futuras

  4. Utilización de la quitina y quitosano como adsorbentes cromatográficos para la separación de alcaloides del extracto en diclorometano de Simaba ferruginea

    OpenAIRE

    Cunha, Georgia P.; Cechinel Filho, Valdir; Domingos T. O. Martins; Marcello, César M.; Joaquim C. S. Lima; Regilane M. Silva; Noldin, Vánia; Rodrigues, Clovis Antonio

    2008-01-01

    Este trabajo presenta un estudio comparativo de la eficacia de diversos adsorbentes cromatográficos en el aislamiento de cantin-6-one y 4 metoxicantin-6-one, dos alcaloides bioactivos aislados del extracto diclorometánico de Simaba ferruginea. Cuando la quitina fue utilizada como adsorbentes de la cromatografía, la producción de los compuestos era más alta que la correspondiente cuando se uso silica gel.

  5. Tip-Pressure-Induced Incoherent Energy Gap in CaFe2As2

    Science.gov (United States)

    Jia-Xin, Yin; Ji-Hui, Wang; Zheng, Wu; Ang, Li; Xue-Jin, Liang; Han-Qing, Mao; Gen-Fu, Chen; Bing, Lv; Ching-Wu, Chu; Hong, Ding; Shu-Heng, Pan

    2016-06-01

    Not Available Supported by the National Natural Science Foundation of China under Grant No 11227903, the National Basic Research Program of China under Grant Nos 2015CB921300 and 2012CB933000, the State of Texas through TcSUH, and the Strategic Priority Research Program B of Chinese Academy of Sciences under Grant Nos XDB07030000, XDB04040300 and Y4VX092X81.

  6. Light vector meson production in pp collisions at sqrt(s) = 7 TeV

    DEFF Research Database (Denmark)

    Collaboration, ALICE; Abelev, B.; Abrahantes Quintana, A.;

    2012-01-01

    The ALICE experiment has measured low-mass dimuon production in pp collisions at \\sqrt{s} = 7 TeV in the dimuon rapidity region 2.5y\\eta^{}, \\phi) into muons and semi-leptonic decays of charmed mesons. The measured production cross sections for \\omega and \\phi are \\sigma_\\omega (1p_t5 GeV/c,2.5y4...

  7. Irrationality via the Hypergeometric method

    Science.gov (United States)

    Bennett, Michael A.

    2008-01-01

    In this paper, we describe how the hypergeometric method of Thue and Siegel may be applied to questions of irrationality. As a consequence of our approach, we provide a somewhat simple proof of a classical theorem of Ljunggren to the effect that the Diophantine equation x2-2y4 = -1 has only the solutions (x,y) = (1,1) and (x,y) = (239,13) in positive integers.

  8. On equal sums of ninth powers

    Science.gov (United States)

    Bremner, A.; Delorme, Jean-Joel

    2010-01-01

    In this paper, we develop an elementary method to obtain infinitely many solutions of the Diophantine equation x_{1}^9+x_{2}^9+x_{3}^9+x_{4}^9+x_{5}^9+x_{6}^9=y_{1}^9+y_{2}^9+y_{3}^9+y_{4}^9+y_{5}^9+y_{6}^9 and we give some numerical results.

  9. Población reproductora del cuervo Corvus corax (L., 1758 en Bizkaia

    Directory of Open Access Journals (Sweden)

    Iñigo Zuberogoitia

    2011-01-01

    Full Text Available Durante las temporadas de reproducción del periodo 1997-2010 se buscaron anualmente los nidos activos de cuervo (Corvus corax de Bizkaia. En total, se localizaron 132 nidos diferentes pertenecientes a 111 territorios, de los que 61 se encontraban en roquedos del interior, 17 en acantilados costeros, 29 en canteras y 4 en áreas boscosas. Todos los territorios localizados se mantuvieron ocupados durante las temporadas posteriores a ser detectados.

  10. Población reproductora del cuervo Corvus corax (L., 1758) en Bizkaia

    OpenAIRE

    Iñigo Zuberogoitia

    2011-01-01

    Durante las temporadas de reproducción del periodo 1997-2010 se buscaron anualmente los nidos activos de cuervo (Corvus corax) de Bizkaia. En total, se localizaron 132 nidos diferentes pertenecientes a 111 territorios, de los que 61 se encontraban en roquedos del interior, 17 en acantilados costeros, 29 en canteras y 4 en áreas boscosas. Todos los territorios localizados se mantuvieron ocupados durante las temporadas posteriores a ser detectados.

  11. Sclerostin stimulates osteocyte support of osteoclast activity by a RANKL-dependent pathway.

    Directory of Open Access Journals (Sweden)

    Asiri R Wijenayaka

    Full Text Available Sclerostin is a product of mature osteocytes embedded in mineralised bone and is a negative regulator of bone mass and osteoblast differentiation. While evidence suggests that sclerostin has an anti-anabolic role, the possibility also exists that sclerostin has catabolic activity. To test this we treated human primary pre-osteocyte cultures, cells we have found are exquisitely sensitive to sclerostin, or mouse osteocyte-like MLO-Y4 cells, with recombinant human sclerostin (rhSCL and measured effects on pro-catabolic gene expression. Sclerostin dose-dependently up-regulated the expression of receptor activator of nuclear factor kappa B (RANKL mRNA and down-regulated that of osteoprotegerin (OPG mRNA, causing an increase in the RANK:OPG mRNA ratio. To examine the effects of rhSCL on resulting osteoclastic activity, MLO-Y4 cells plated onto a bone-like substrate were primed with rhSCL for 3 days and then either mouse splenocytes or human peripheral blood mononuclear cells (PBMC were added. This resulted in cultures with elevated osteoclastic resorption (approximately 7-fold compared to untreated co-cultures. The increased resorption was abolished by co-addition of recombinant OPG. In co-cultures of MLO-Y4 cells with PBMC, SCL also increased the number and size of the TRAP-positive multinucleated cells formed. Importantly, rhSCL had no effect on TRAP-positive cell formation from monocultures of either splenocytes or PBMC. Further, rhSCL did not induce apoptosis of MLO-Y4 cells, as determined by caspase activity assays, demonstrating that the osteoclastic response was not driven by dying osteocytes. Together, these results suggest that sclerostin may have a catabolic action through promotion of osteoclast formation and activity by osteocytes, in a RANKL-dependent manner.

  12. Metales pesados y sus implicaciones en la calidad del suelo

    OpenAIRE

    García, Carlos; Moreno, José Luis; Hernández, M. Teresa; Polo, Alfredo

    2002-01-01

    14 páginas, 2 figuras y 4 tablas Ponencia que forma parte de las "Segundas jornadas científicas sobre medio ambiente del CCMA-CSIC" (Abril-2002) "Cambio global, ecología, contaminación, restauración, la agricultura del nuevo milenio, salud y medio ambiente, nuevas perspectivas desde la bioquímica y la biología molecular"

  13. RANGO CRITICO DE NITRÓGENO Y POTASIO EN HOJAS INDICADORAS PARA DOS ÉPOCAS DE PLANTACIÓN EN EL CULTIVO PROTEGIDO DEL TOMATE (Solanum lycopersicum L. HIBRIDO HA-3019

    Directory of Open Access Journals (Sweden)

    María I. Hernández

    2009-01-01

    5.20 % (EI, mientras que en plena producción fue de 2.56-2.84 % (EPV y 3.24-3.22 % (EI. El RCN para K en la fase III osciló entre 4.90-4.96 % (EPV y 4.29-4.35 (EI, mientras que en plena producción se cuantificaron valores entre 3.34¿3.47 % (EPV y 3.69-3.96 % (EI.

  14. 神经肽Y(NPY)的生理功能研究进展%Overview on the physiological functions of neuropeptide Y(NPY)

    Institute of Scientific and Technical Information of China (English)

    胡春燕; 李英文

    2011-01-01

    Neuropeptide Y (NPY) is an important neurotransmitter in the body, and very conservative. Generally existing as precursors, after releasing, active NPY could play an important role mainly through binding to receptors. About NPY receptors, there are eight subtypes named Y1, Y2, Y3, Y4, Y5, Y6, Y7 and Y8. Y1 and Y2 are keys in the contraction of blood vessels; Y1, Y2 and Y5 are the key receptors on animal feeding; in anxiety and depression behavior; Y1 , Y2 and Y4 are the necessary conditions that NPY regulate animal, depressed behavior. This paper focuses on describing how to play the role of NPY in related physiological functions of animals after binding with receptors.%神经肤Y(NPY)是机体内的一种重要且保守的神经递质,一般以前体形式存在,释放的有活性的NPY主要通过与其受体结合发挥作用.NPY受体包含了亚型Y1、Y2、Y3、Y4、Y5、Y6、Y7、Y8.Y1和Y2是NPY发挥收缩血管作用的关键受体;Y1、Y2和Y5是NPY调节动物摄食行为的关键受体;Y1、Y2和Y4是NPY调控动物焦虑、沮丧行为的必要受体.着重对NPY与其各种受体结合后如何行使动物的相关生理功能的情况进行了阐述.

  15. Outcome tools used for ambulatory children with cerebral palsy: responsiveness and minimum clinically important differences

    OpenAIRE

    Oeffinger, D; Bagley, A; Rogers, S.; Gorton, G; Kryscio, R; Abel, M.; Damiano, D; Barnes, D.; Tylkowski, C

    2008-01-01

    This prospective longitudinal multicenter study of ambulatory children with cerebral palsy (CP) examined changes in outcome tool score over time, tool responsiveness, and used a systematic method for defining minimum clinically important differences (MCIDs). Three hundred and eighty-one participants with CP (Gross Motor Function Classification System [GMFCS] Levels I–III; age range 4–18y, mean age 11y [SD 4y 4mo]; 265 diplegia, 116 hemiplegia; 230 males, 151 females). At baseline and follow-u...

  16. β1 Integrins Mediate Mechanosensitive Signaling Pathways in Osteocytes

    OpenAIRE

    Litzenberger, Julie B.; Tummala, Padmaja; Kim, Jae-Beom; Jacobs, Christopher R.

    2010-01-01

    Integrins are cell-substrate adhesion proteins that initiate intracellular signaling and may serve as mechanosensors in bone. MLO-Y4 cells were stably transfected with a dominant negative form of the β1 integrin subunit (β1DN) containing the transmembrane domain and cytoplasmic tail of β1 integrin. Cells expressing β1DN had reduced vinculin localization to focal contacts but no change in intracellular actin organization. When exposed to oscillatory fluid flow, β1DN cells exhibited a significa...

  17. Adenylyl cyclase 6 mediates loading-induced bone adaptation in vivo

    OpenAIRE

    Kristen L Lee; Hoey, David A.; Spasic, Milos; Tang, Tong; Hammond, H. Kirk; Jacobs, Christopher R.

    2014-01-01

    Primary cilia are single, nonmotile, antenna-like structures extending from the apical membrane of most mammalian cells. They may mediate mechanotransduction, the conversion of external mechanical stimuli into biochemical intracellular signals. Previously we demonstrated that adenylyl cyclase 6 (AC6), a membrane-bound enzyme enriched in primary cilia of MLO-Y4 osteocyte-like cells, may play a role in a primary cilium-dependent mechanism of osteocyte mechanotransduction in vitro. In this study...

  18. The role of the sphingosine-1-phosphate signaling pathway in osteocyte mechanotransduction.

    Science.gov (United States)

    Zhang, Jia-Ning; Zhao, Yan; Liu, Chao; Han, Elizabeth S; Yu, Xue; Lidington, Darcy; Bolz, Steffen-Sebastian; You, Lidan

    2015-10-01

    Osteocytes are proposed to be the mechanosensory cells that translate mechanical loading into biochemical signals during the process of bone adaptation. The lipid mediator sphingosine-1-phosphate (S1P) has been reported to play a role in the mechanotransduction process of blood vessels and also in the dynamic control of bone mineral homeostasis. Nevertheless, the potential role of S1P in bone mechanotransduction has yet to be elucidated. In this study, we hypothesized that a S1P cascade is involved in the activation of osteocytes in response to loading-induced oscillatory fluid flow (OFF) in bone. MLO-Y4 osteocyte-like cells express the necessary components of a functional S1P cascade. To examine the involvement of S1P signaling in osteocyte mechanotransduction, we applied OFF (1 Pa, 1 Hz) to osteocyte-like MLO-Y4 cells under conditions where the S1P signaling pathway was modulated. We found that decreased endogenous S1P levels significantly suppressed the OFF-induced intracellular calcium response. Addition of extracellular S1P to MLO-Y4 cells enhanced the synthesis and release of prostaglandin E2 (PGE2) under static cells and amplified OFF-induced PGE2 release. The stimulatory effect of OFF on the gene expression levels of osteoprotegerin (OPG) and receptor activator for nuclear factor κB ligand (RANKL) was S1P dependent. Furthermore, the S1P2 receptor subtype was shown to be involved in OFF-induced PGE2 synthesis and release, as well as down-regulation of RANKL/OPG gene expression ratio. In summary, our data suggest that S1P cascade is involved in OFF-induced mechanotransduction in MLO-Y4 cells and that extracellular S1P exerts its effect partly through S1P2 receptors. PMID:25988659

  19. Calcium Response in Osteocytic Networks under Steady and Oscillatory Fluid Flow

    OpenAIRE

    Lu, X. Lucas; Huo, Bo; Park, Miri; Guo, X. Edward

    2012-01-01

    The fluid flow in the lacunar-canalicular system of bone is an essential mechanical stimulation on the osteocyte networks. Due to the complexity of human physical activities, the fluid shear stress on osteocyte bodies and processes consists of both steady and oscillatory components. In this study, we investigated and compared the intracellular calcium ([Ca2+]i) responses of osteocytic networks under steady and oscillatory fluid flows. An in vitro osteocytic network was built with MLO-Y4 osteo...

  20. Mechanical Strain Opens Connexin 43 Hemichannels in Osteocytes: A Novel Mechanism for the Release of ProstaglandinD⃞

    OpenAIRE

    Cherian, Priscilla P.; Siller-Jackson, Arlene J.; Gu, Sumin; Wang, Xin; Bonewald, Lynda F.; Sprague, Eugene; Jiang, Jean X.

    2005-01-01

    Mechanosensing bone osteocytes express large amounts of connexin (Cx)43, the component of gap junctions; yet, gap junctions are only active at the small tips of their dendritic processes, suggesting another function for Cx43. Both primary osteocytes and the osteocyte-like MLO-Y4 cells respond to fluid flow shear stress by releasing intracellular prostaglandin E2 (PGE2). Cells plated at lower densities release more PGE2 than cells plated at higher densities. This response was significantly red...

  1. Mechanical Induction of PGE2 in Osteocytes Blocks Glucocorticoid-Induced Apoptosis Through Both the β-Catenin and PKA Pathways

    OpenAIRE

    Kitase, Yukiko; Barragan, Leonardo; Qing, Hai; Kondoh, Shino; Jiang, Jean X.; Johnson, Mark L.; Bonewald, Lynda F.

    2010-01-01

    Glucocorticoids are known to induce osteocyte apoptosis, whereas mechanical loading has been shown to sustain osteocyte viability. Here we show that mechanical loading in the form of fluid-flow shear stress blocks dexamethasone-induced apoptosis of osteocyte-like cells (MLO-Y4). Prostaglandin E2 (PGE2), a rapidly induced signaling molecule produced by osteocytes, was shown to be protective against dexamethasone-induced apoptosis, whereas indomethacin reversed the antiapoptotic effects of shea...

  2. Unfractionated Heparin Promotes Osteoclast Formation in Vitro by Inhibiting Osteoprotegerin Activity

    OpenAIRE

    Binghan Li; Dan Lu; Yuqing Chen; Minghui Zhao; Li Zuo

    2016-01-01

    Heparin has been proven to enhance bone resorption and induce bone loss. Since osteoclasts play a pivotal role in bone resorption, the effect of heparin on osteoclastogenesis needs to be clarified. Since osteocytes are the key modulator during osteoclastogenesis, we evaluated heparin’s effect on osteoclastogenesis in vitro by co-culturing an osteocyte cell line (MLO-Y4) and pre-osteoclasts (RAW264.7). In this co-culture system, heparin enhanced osteoclastogenesis and osteoclastic bone resorpt...

  3. On the electrophysiological response of bone cells using a Stokesian fluid stimulus probe for delivery of quantifiable localized picoNewton level forces

    OpenAIRE

    Wu, Danielle; Ganatos, Peter; SPRAY, DAVID C.; Weinbaum, Sheldon

    2011-01-01

    A Stokesian fluid stimulus probe (SFSP), capable of delivering quantifiable pN level hydrodynamic forces, is developed to distinguish the electrophysiological response of the cell process and cell body of osteocyte-like MLO-Y4 cells without touching the cell or its substrate. The hydrodynamic disturbance is a short lived (100 ms), constant strength pressure pulse that propagates nearly instantaneously through the medium creating a nearly spherical expanding fluid bolus surrounding a 0.8 µm mi...

  4. Prevention of osteocyte and osteoblast apoptosis by bisphosphonates and calcitonin

    OpenAIRE

    Plotkin, Lilian I.; Weinstein, Robert S; Parfitt, A. Michael; Roberson, Paula K.; Manolagas, Stavros C.; Bellido, Teresita

    1999-01-01

    Glucocorticoid-induced osteoporosis may be due, in part, to increased apoptosis of osteocytes and osteoblasts, and bisphosphonates (BPs) are effective in the management of this condition. We have tested the hypothesis that BPs suppress apoptosis in these cell types. Etidronate, alendronate, pamidronate, olpadronate, or amino-olpadronate (IG9402, a bisphosphonate that lacks antiresorptive activity) at 10–9 to 10–6 M prevented apoptosis of murine osteocytic MLO-Y4 cells, whether it was induced ...

  5. FGF and ERK signaling coordinately regulate mineralization-related genes and play essential roles in osteocyte differentiation.

    Science.gov (United States)

    Kyono, Ai; Avishai, Nanthawan; Ouyang, Zhufeng; Landreth, Gary E; Murakami, Shunichi

    2012-01-01

    To examine the roles of FGF and ERK MAPK signaling in osteocyte differentiation and function, we performed microarray analyses using the osteocyte cell line MLO-Y4. This experiment identified a number of mineralization-related genes that were regulated by FGF2 in an ERK MAPK-dependent manner. Real-time PCR analysis indicated that FGF2 upregulates Ank, Enpp1, Mgp, Slc20a1, and Dmp1 in MLO-Y4 cells. Consistent with this observation, the selective FGF receptor inhibitor PD173074 decreased Ank, Enpp1, Slc20a1, and Dmp1 mRNA expression in mouse calvaria in organ culture. Since Dmp1 plays a central role in osteocyte differentiation and mineral homeostasis, we further analyzed FGF regulation of Dmp1. Similar to FGF2, FGF23 upregulated Dmp1 expression in MLO-Y4 cells in the presence of Klotho. Furthermore, increased extracellular phosphate levels partially inhibited FGF2-induced upregulation of Dmp1 mRNA expression, suggesting a coordinated regulation of Dmp1 expression by FGF signaling and extracellular phosphate. In MLO-Y4 osteocytes and in MC3T3E1 and primary calvaria osteoblasts, U0126 strongly inhibited both basal expression of Dmp1 mRNA and FGF2-induced upregulation. Consistent with the in vitro observations, real-time PCR and immunohistochemical analysis showed a strong decrease in Dmp1 expression in the skeletal elements of ERK1(-/-); ERK2(flox/flox); Prx1-Cre mice. Furthermore, scanning electron microscopic analysis revealed that no osteocytes with characteristic dendritic processes develop in the limbs of ERK1(-/-); ERK2 (flox/flox); Prx1-Cre mice. Collectively, our observations indicate that FGF signaling coordinately regulates mineralization-related genes in the osteoblast lineage and that ERK signaling is essential for Dmp1 expression and osteocyte differentiation. PMID:21678127

  6. Identification of Osteocyte-Selective Proteins

    OpenAIRE

    Guo, Dayong; Keightley, Andrew; Guthrie, Jill; Veno, Patricia A.; Harris, Stephen E.; Bonewald, Lynda F.

    2010-01-01

    Since little is known regarding osteocytes, cells embedded within the mineralized bone matrix, a proteomics approach was used to discover proteins more highly expressed in osteocytes than in osteoblasts to determine osteocyte specific function. Two proteomic profiles obtained by two different proteomic approaches using total cell lysates from the osteocyte cell line MLO-Y4 and the osteoblast cell line MC3T3 revealed unique differences. Three protein clusters, one related to glycolysis, (Phosp...

  7. Measurement of B9Upsilon(5S) to Bs(*) Bs(*)bar Using phi Mesons

    CERN Document Server

    Huang, G; Pavlunin, V; Sanghi, B; Shipsey, I P J; Xin, B; Adams, G S; Anderson, M; Cummings, J P; Danko, I; Napolitano, J; He, Q; Insler, J; Muramatsu, H; Park, C S; Thorndike, E H; Yang, F; Coan, T E; Gao, Y S; Liu, F; Artuso, M; Blusk, S; Butt, J; Li, J; Menaa, N; Mountain, R; Nisar, S; Randrianarivony, K; Redjimi, R; Sia, R; Skwarnicki, T; Stone, S; Wang, J C; Zhang, K; Csorna, S E; Bonvicini, G; Cinabro, D; Dubrovin, M; Lincoln, A; Asner, D M; Edwards, K W; Briere, R A; Brock, I; Chen, J; Ferguson, T; Tatishvili, G T; Vogel, H; Watkins, M E; Rosner, J L; Adam, N E; Alexander, J P; Berkelman, K; Cassel, D G; Duboscq, J E; Ecklund, K M; Ehrlich, R; Fields, L; Gibbons, L; Gray, R; Gray, S W; Hartill, D L; Heltsley, B K; Hertz, D; Jones, C D; Kandaswamy, J; Kreinick, D L; Kuznetsov, V E; Mahlke-Krüger, H; Onyisi, P U E; Patterson, J R; Peterson, D; Pivarski, J; Riley, D; Ryd, A; Sadoff, A J; Schwarthoff, H; Shi, X; Stroiney, S; Sun, W M; Wilksen, T; Weinberger, M; Athar, S B; Patel, R; Potlia, V; Yelton, J; Rubin, P; Cawlfield, C; Eisenstein, B I; Karliner, I; Kim, D; Lowrey, N; Naik, P; Sedlack, C; Selen, M; White, E J; Wiss, J; Shepherd, M R; Besson, D; Pedlar, T K; Cronin-Hennessy, D; Gao, K Y; Gong, D T; Hietala, J; Kubota, Y; Klein, T; Lang, B W; Poling, R; Scott, A W; Smith, A; Zweber, P; Dobbs, S; Metreveli, Z V; Seth, K K; Tomaradze, A G; Ernst, J; Severini, H; Dytman, S A; Love, W; Savinov, V; Aquines, O; Li, Z; López, A; Mehrabyan, S S; Méndez, H; Ramírez, J; al, et

    2006-01-01

    Knowledge of the Bs decay fraction of the Y(5S) resonance, fs, is important for Bs meson studies at the Y(5S) energy. Using data collected by the CLEO III detector at CESR consisting of 0.423 1/fb on the Y(5S) resonance, 6.34 1/fb on the Y(4S) and 2.32 1/fb in the continuum below the Y(4S), we measure B(Y(5S)-> phi X)=(13.2+/- 0.7 ^{+2.2}_{-1.4})% and B(Y (4S)-> phi X)=(7.1 +/- 0.1 +/- 0.6)%, the ratio of the two rates is (1.9 +/- 0.1 ^{+0.3}_{-0.2}). This is the first measurement of the phi meson yield from the Y(5S). Using these rates, and a model dependent estimate of B(Bs -> phi X), we measure fs=(27.3 +/- 3.2 ^{+14.6}_{-~6.1})%. We update our previous, independent measurement of this branching fraction using the inclusive Ds yields to be (21.8 +/- 3.4 ^{+8.5}_{-4.2})%, due to changes in the $D_s^+ -> phi \\pi^+$ branching fraction and a better estimate of the number of hadronic events. We also report the total Y(5S) hadronic cross section above continuum to be sigma(e^+e^- -> Y(5S))=(0.301 +/- 0.002 +/- 0...

  8. Measurement of B[Y(5S)->Bs(*) anti-Bs(*)] Using phi Mesons

    CERN Document Server

    Huang, G S; Adams, G S; Alexander, J P; Anderson, M; Aquines, O; Artuso, M; Asner, D M; Athar, S B; Berkelman, K; Besson, D; Blusk, S; Bonvicini, G; Briere, R A; Brock, I; Butt, J; Cassel, D G; Cawlfield, C; Chen, J; Cinabro, D; Coan, T E; Cronin-Hennessy, D; Csorna, S E; Cummings, J P; Danko, I; Dobbs, S; Duboscq, J E; Dubrovin, M; Dytman, S A; Ecklund, K M; Edwards, K W; Ehrlich, R; Eisenstein, B I; Ernst, J; Ferguson, T; Fields, L; Gao, K Y; Gao, Y S; Gibbons, L; Gong, D T; Gray, R; Gray, S W; Hartill, D L; He, Q; Heltsley, B K; Hertz, D; Hietala, J; Huang, G S; Insler, J; Jones, C D; Kandaswamy, J; Karliner, I; Kim, D; Klein, T; Kreinick, D L; Kubota, Y; Kuznetsov, V E; Lang, B W; Li, J; Li, Z; Lincoln, A; Liu, F; Love, W; Lowrey, N; López, A; Mahlke-Krüger, H; Mehrabyan, S S; Menaa, N; Metreveli, Z V; Miller, D H; Mountain, R; Muramatsu, H; Méndez, H; Naik, P; Napolitano, J; Nisar, S; Onyisi, P U E; Park, C S; Patel, R; Patterson, J R; Pavlunin, V; Pedlar, T K; Peterson, D; Pivarski, J; Poling, R; Potlia, V; Ramírez, J; Randrianarivony, K; Redjimi, R; Riley, D; Rosner, J L; Rubin, P; Ryd, A; Sadoff, A J; Sanghi, B; Savinov, V; Schwarthoff, H; Scott, A W; Sedlack, C; Selen, M; Seth, K K; Severini, H; Shepherd, M R; Shi, X; Shipsey, I P J; Sia, R; Skwarnicki, T; Smith, A; Stone, S; Stroiney, S; Sun, W M; Tatishvili, G T; Thorndike, E H; Tomaradze, A G; Vogel, H; Wang, J C; Watkins, M E; Weinberger, M; White, E J; Wilksen, T; Wiss, J; Xin, B; Yang, F; Yelton, J; Zhang, K; Zweber, P; al., et

    2007-01-01

    Knowledge of the Bs decay fraction of the Y(5S) resonance, fs, is important for Bs meson studies at the Y(5S) energy. Using a data sample collected by the CLEO III detector at CESR consisting of 0.423/fb on the Y(5S) resonance, 6.34/fb on the Y(4S) and 2.32/fb in the continuum below the Y(4S), we measure B(Y(5S) -> phi X)=(13.8 +/- 0.7 {+2.3}{-1.5})% and B(Y(4S) -> phi X) = (7.1 +/- 0.1 +/-0.6)%; the ratio of the two rates is (1.9 +/- 0.1 {+0.3}{-0.2}). This is the first measurement of the phi meson yield from the Y(5S). Using these rates, and a model dependent estimate of B(Bs -> phi X), we determine fs = (24.6 +/- 2.9 {+11.0}{-5.3})%. We also update our previous independent measurement of fs made using the inclusive Ds yields to now be (16.8 +/- 2.6 {+6.7}{-3.4)%, due to a better estimate of the number of hadronic events. We also report the total Y(5S) hadronic cross section above continuum to be sigma(e^+e^- -> Y(5S))=(0.301 +/- 0.002 +/- 0.039) nb. This allows us to extract the fraction of B mesons as (58...

  9. Light vector meson production in pp collisions at $\\sqrt{s}$ = 7 TeV

    CERN Document Server

    Abelev, Betty; Adamova, Dagmar; Adare, Andrew Marshall; Aggarwal, Madan; Aglieri Rinella, Gianluca; Agocs, Andras Gabor; Agostinelli, Andrea; Aguilar Salazar, Saul; Ahammed, Zubayer; Ahmad, Arshad; Ahmad, Nazeer; Ahn, Sang Un; Akindinov, Alexander; Aleksandrov, Dmitry; Alessandro, Bruno; Alfaro Molina, Jose Ruben; Alici, Andrea; Alkin, Anton; Almaraz Avina, Erick Jonathan; Alme, Johan; Alt, Torsten; Altini, Valerio; Altinpinar, Sedat; Altsybeev, Igor; Andrei, Cristian; Andronic, Anton; Anguelov, Venelin; Anielski, Jonas; Anticic, Tome; Antinori, Federico; Antonioli, Pietro; Aphecetche, Laurent Bernard; Appelshauser, Harald; Arbor, Nicolas; Arcelli, Silvia; Arend, Andreas; Armesto, Nestor; Arnaldi, Roberta; Aronsson, Tomas Robert; Arsene, Ionut Cristian; Arslandok, Mesut; Asryan, Andzhey; Augustinus, Andre; Averbeck, Ralf Peter; Awes, Terry; Aysto, Juha Heikki; Azmi, Mohd Danish; Bach, Matthias Jakob; Badala, Angela; Baek, Yong Wook; Bailhache, Raphaelle Marie; Bala, Renu; Baldini Ferroli, Rinaldo; Baldisseri, Alberto; Baldit, Alain; Baltasar Dos Santos Pedrosa, Fernando; Ban, Jaroslav; Baral, Rama Chandra; Barbera, Roberto; Barile, Francesco; Barnafoldi, Gergely Gabor; Barnby, Lee Stuart; Barret, Valerie; Bartke, Jerzy Gustaw; Basile, Maurizio; Bastid, Nicole; Bathen, Bastian; Batigne, Guillaume; Batyunya, Boris; Baumann, Christoph Heinrich; Bearden, Ian Gardner; Beck, Hans; Belikov, Iouri; Bellini, Francesca; Bellwied, Rene; Belmont-Moreno, Ernesto; Beole, Stefania; Berceanu, Ionela; Bercuci, Alexandru; Berdnikov, Yaroslav; Berenyi, Daniel; Bergmann, Cyrano; Berzano, Dario; Betev, Latchezar; Bhasin, Anju; Bhati, Ashok Kumar; Bianchi, Livio; Bianchi, Nicola; Bianchin, Chiara; Bielcik, Jaroslav; Bielcikova, Jana; Bilandzic, Ante; Blanco, F; Blanco, Francesco; Blau, Dmitry; Blume, Christoph; Bock, Nicolas; Bogdanov, Alexey; Boggild, Hans; Bogolyubsky, Mikhail; Boldizsar, Laszlo; Bombara, Marek; Book, Julian; Borel, Herve; Borissov, Alexander; Bortolin, Claudio; Bose, Suvendu Nath; Bossu, Francesco; Botje, Michiel; Bottger, Stefan; Boyer, Bruno Alexandre; Braun-Munzinger, Peter; Bregant, Marco; Breitner, Timo Gunther; Broz, Michal; Brun, Rene; Bruna, Elena; Bruno, Giuseppe Eugenio; Budnikov, Dmitry; Buesching, Henner; Bufalino, Stefania; Bugaiev, Kyrylo; Busch, Oliver; Buthelezi, Edith Zinhle; Caffarri, Davide; Cai, Xu; Caines, Helen Louise; Calvo Villar, Ernesto; Camerini, Paolo; Canoa Roman, Veronica; Cara Romeo, Giovanni; Carena, Francesco; Carena, Wisla; Carminati, Federico; Casanova Diaz, Amaya Ofelia; Caselle, Michele; Castillo Castellanos, Javier Ernesto; Casula, Ester Anna Rita; Catanescu, Vasile; Cavicchioli, Costanza; Cepila, Jan; Cerello, Piergiorgio; Chang, Beomsu; Chapeland, Sylvain; Charvet, Jean-Luc Fernand; Chattopadhyay, Sukalyan; Chattopadhyay, Subhasis; Cherney, Michael Gerard; Cheshkov, Cvetan; Cheynis, Brigitte; Chiavassa, Emilio; Chibante Barroso, Vasco Miguel; Chinellato, David; Chochula, Peter; Chojnacki, Marek; Christakoglou, Panagiotis; Christensen, Christian Holm; Christiansen, Peter; Chujo, Tatsuya; Chung, Suh-Urk; Cicalo, Corrado; Cifarelli, Luisa; Cindolo, Federico; Cleymans, Jean Willy Andre; Coccetti, Fabrizio; Coffin, Jean-Pierre Michel; Colamaria, Fabio; Colella, Domenico; Conesa Balbastre, Gustavo; Conesa del Valle, Zaida; Constantin, Paul; Contin, Giacomo; Contreras, Jesus Guillermo; Cormier, Thomas Michael; Corrales Morales, Yasser; Cortes Maldonado, Ismael; Cortese, Pietro; Cosentino, Mauro Rogerio; Costa, Filippo; Cotallo, Manuel Enrique; Crochet, Philippe; Cruz Alaniz, Emilia; Cuautle, Eleazar; Cunqueiro, Leticia; D'Erasmo, Ginevra; Dainese, Andrea; Dalsgaard, Hans Hjersing; Danu, Andrea; Das, Debasish; Das, Indranil; Das, Kushal; Dash, Ajay Kumar; Dash, Sadhana; De, Sudipan; De Azevedo Moregula, Andrea; de Barros, Gabriel; De Caro, Annalisa; de Cataldo, Giacinto; de Cuveland, Jan; De Falco, Alessandro; De Gruttola, Daniele; De Marco, Nora; De Pasquale, Salvatore; de Rooij, Raoul Stefan; Del Castillo Sanchez, Eduardo; Delagrange, Hugues; Deloff, Andrzej; Demanov, Vyacheslav; Denes, Ervin; Deppman, Airton; Di Bari, Domenico; Di Giglio, Carmelo; Di Liberto, Sergio; Di Mauro, Antonio; Di Nezza, Pasquale; Dietel, Thomas; Divia, Roberto; Djuvsland, Oeystein; Dobrin, Alexandru Florin; Dobrowolski, Tadeusz Antoni; Dominguez, Isabel; Donigus, Benjamin; Dordic, Olja; Driga, Olga; Dubey, Anand Kumar; Ducroux, Laurent; Dupieux, Pascal; Dutta Majumdar, AK; Dutta Majumdar, Mihir Ranjan; Elia, Domenico; Emschermann, David Philip; Engel, Heiko; Erdal, Hege Austrheim; Espagnon, Bruno; Estienne, Magali Danielle; Esumi, Shinichi; Evans, David; Eyyubova, Gyulnara; Fabris, Daniela; Faivre, Julien; Falchieri, Davide; Fantoni, Alessandra; Fasel, Markus; Fearick, Roger Worsley; Fedunov, Anatoly; Fehlker, Dominik; Feldkamp, Linus; Felea, Daniel; Fenton-Olsen, Bo; Feofilov, Grigory; Fernandez Tellez, Arturo; Ferretti, Alessandro; Ferretti, Roberta; Figiel, Jan; Figueredo, Marcel; Filchagin, Sergey; Fini, Rosa Anna; Finogeev, Dmitry; Fionda, Fiorella; Fiore, Enrichetta Maria; Floris, Michele; Foertsch, Siegfried Valentin; Foka, Panagiota; Fokin, Sergey; Fragiacomo, Enrico; Fragkiadakis, Michail; Frankenfeld, Ulrich Michael; Fuchs, Ulrich; Furget, Christophe; Fusco Girard, Mario; Gaardhoje, Jens Joergen; Gagliardi, Martino; Gago, Alberto; Gallio, Mauro; Gangadharan, Dhevan Raja; Ganoti, Paraskevi; Garabatos, Jose; Garcia-Solis, Edmundo; Garishvili, Irakli; Gerhard, Jochen; Germain, Marie; Geuna, Claudio; Gheata, Andrei George; Gheata, Mihaela; Ghidini, Bruno; Ghosh, Premomoy; Gianotti, Paola; Girard, Martin Robert; Giubellino, Paolo; Gladysz-Dziadus, Ewa; Glassel, Peter; Gomez, Ramon; Gonzalez Ferreiro, Elena; Gonzalez-Trueba, Laura Helena; Gonzalez-Zamora, Pedro; Gorbunov, Sergey; Goswami, Ankita; Gotovac, Sven; Grabski, Varlen; Graczykowski, Lukasz Kamil; Grajcarek, Robert; Grelli, Alessandro; Grigoras, Alina Gabriela; Grigoras, Costin; Grigoriev, Vladislav; Grigoryan, Ara; Grigoryan, Smbat; Grinyov, Boris; Grion, Nevio; Grosse-Oetringhaus, Jan Fiete; Grossiord, Jean-Yves; Grosso, Raffaele; Guber, Fedor; Guernane, Rachid; Guerra Gutierrez, Cesar; Guerzoni, Barbara; Guilbaud, Maxime Rene Joseph; Gulbrandsen, Kristjan Herlache; Gunji, Taku; Gupta, Anik; Gupta, Ramni; Gutbrod, Hans; Haaland, Oystein Senneset; Hadjidakis, Cynthia Marie; Haiduc, Maria; Hamagaki, Hideki; Hamar, Gergoe; Hanratty, Luke David; Hansen, Alexander; Harmanova, Zuzana; Harris, John William; Hartig, Matthias; Hasegan, Dumitru; Hatzifotiadou, Despoina; Hayrapetyan, Arsen; Heide, Markus Ansgar; Helstrup, Haavard; Herghelegiu, Andrei Ionut; Herrera Corral, Gerardo Antonio; Herrmann, Norbert; Hetland, Kristin Fanebust; Hicks, Bernard; Hille, Per Thomas; Hippolyte, Boris; Horaguchi, Takuma; Hori, Yasuto; Hristov, Peter Zahariev; Hrivnacova, Ivana; Huang, Meidana; Huber, Sebastian Bernd; Humanic, Thomas; Hwang, Dae Sung; Ichou, Raphaelle; Ilkaev, Radiy; Ilkiv, Iryna; Inaba, Motoi; Incani, Elisa; Innocenti, Gian Michele; Ippolitov, Mikhail; Irfan, Muhammad; Ivan, Cristian George; Ivanov, Andrey; Ivanov, Marian; Ivanov, Vladimir; Ivanytskyi, Oleksii; Jacholkowski, Adam Wlodzimierz; Jacobs, Peter; Jancurova, Lucia; Jangal, Swensy Gwladys; Janik, Malgorzata Anna; Janik, Rudolf; Jayarathna, Sandun; Jena, Satyajit; Jimenez Bustamante, Raul Tonatiuh; Jirden, Lennart; Jones, Peter Graham; Jung, Hyung Taik; Jung, Won Woong; Jusko, Anton; Kakoyan, Vanik; Kalcher, Sebastian; Kalinak, Peter; Kalisky, Matus; Kalliokoski, Tuomo Esa Aukusti; Kalweit, Alexander Philipp; Kanaki, Kalliopi; Kang, Ju Hwan; Kaplin, Vladimir; Karasu Uysal, Ayben; Karavichev, Oleg; Karavicheva, Tatiana; Karpechev, Evgeny; Kazantsev, Andrey; Kebschull, Udo Wolfgang; Keidel, Ralf; Khan, Mohisin Mohammed; Khan, Palash; Khan, Shuaib Ahmad; Khanzadeev, Alexei; Kharlov, Yury; Kileng, Bjarte; Kim, Beomkyu; Kim, Dong Jo; Kim, Do Won; Kim, Jonghyun; Kim, Jin Sook; Kim, Minwoo; Kim, Se Yong; Kim, Seon Hee; Kim, Taesoo; Kirsch, Stefan; Kisel, Ivan; Kiselev, Sergey; Kisiel, Adam Ryszard; Klay, Jennifer Lynn; Klein, Jochen; Klein-Bosing, Christian; Kliemant, Michael; Kluge, Alexander; Knichel, Michael Linus; Koch, Kathrin; Kohler, Markus; Kolojvari, Anatoly; Kondratiev, Valery; Kondratyeva, Natalia; Konevskih, Artem; Korneev, Andrey; Kottachchi Kankanamge Don, Chamath; Kour, Ravjeet; Kowalski, Marek; Kox, Serge; Koyithatta Meethaleveedu, Greeshma; Kral, Jiri; Kralik, Ivan; Kramer, Frederick; Kraus, Ingrid Christine; Krawutschke, Tobias; Kretz, Matthias; Krivda, Marian; Krizek, Filip; Krus, Miroslav; Kryshen, Evgeny; Krzewicki, Mikolaj; Kucheriaev, Yury; Kuhn, Christian Claude; Kuijer, Paul; Kurashvili, Podist; Kurepin, A; Kurepin, AB; Kuryakin, Alexey; Kushpil, Svetlana; Kushpil, Vasily; Kweon, Min Jung; Kwon, Youngil; La Rocca, Paola; Ladron de Guevara, Pedro; Lakomov, Igor; Langoy, Rune; Lara, Camilo Ernesto; Lardeux, Antoine Xavier; Lazzeroni, Cristina; Le Bornec, Yves; Lea, Ramona; Lechman, Mateusz; Lee, Ki Sang; Lee, Sung Chul; Lefevre, Frederic; Lehnert, Joerg Walter; Leistam, Lars; Lenhardt, Matthieu Laurent; Lenti, Vito; Leon Monzon, Ildefonso; Leon Vargas, Hermes; Leoncino, Marco; Levai, Peter; Li, Xiaomei; Lien, Jorgen; Lietava, Roman; Lindal, Svein; Lindenstruth, Volker; Lippmann, Christian; Lisa, Michael Annan; Liu, Lijiao; Loenne, Per-Ivar; Loggins, Vera; Loginov, Vitaly; Lohn, Stefan Bernhard; Lohner, Daniel; Loizides, Constantinos; Loo, Kai Krister; Lopez, Xavier Bernard; Lopez Torres, Ernesto; Lovhoiden, Gunnar; Lu, Xianguo; Luettig, Philipp; Lunardon, Marcello; Luo, Jiebin; Luparello, Grazia; Luquin, Lionel; Luzzi, Cinzia; Ma, Rongrong; Maevskaya, Alla; Mager, Magnus; Mahapatra, Durga Prasad; Maire, Antonin; Mal'Kevich, Dmitry; Malaev, Mikhail; Maldonado Cervantes, Ivonne Alicia; Malinina, Ludmila; Malzacher, Peter; Mamonov, Alexander; Manceau, Loic Henri Antoine; Manko, Vladislav; Manso, Franck; Manzari, Vito; Mao, Yaxian; Marchisone, Massimiliano; Mares, Jiri; Margagliotti, Giacomo Vito; Margotti, Anselmo; Marin, Ana Maria; Markert, Christina; Martashvili, Irakli; Martinengo, Paolo; Martinez, Mario Ivan; Martinez Davalos, Arnulfo; Martinez Garcia, Gines; Martynov, Yevgen; Mas, Alexis Jean-Michel; Masciocchi, Silvia; Masera, Massimo; Maslov, Nikolai; Masoni, Alberto; Massacrier, Laure Marie; Mastromarco, Mario; Mastroserio, Annalisa; Matthews, Zoe Louise; Matyja, Adam Tomasz; Mayani, Daniel; Mayer, Christoph; Mazer, Joel; Mazzoni, Alessandra Maria; Meddi, Franco; Menchaca-Rocha, Arturo Alejandro; Mercado Perez, Jorge; Meres, Michal; Miake, Yasuo; Michalon, Alain; Midori, Jumpei; Milano, Leonardo; Milosevic, Jovan; Mischke, Andre; Mishra, Aditya Nath; Miskowiec, Dariusz; Mitu, Ciprian Mihai; Mlynarz, Jocelyn; Mohanty, Ajit Kumar; Mohanty, Bedangadas; Molnar, Levente; Montano Zetina, Luis Manuel; Monteno, Marco; Montes, Esther; Moon, Taebong; Morando, Maurizio; Moreira De Godoy, Denise Aparecida; Moretto, Sandra; Morsch, Andreas; Muccifora, Valeria; Mudnic, Eugen; Muhuri, Sanjib; Muller, Hans; Munhoz, Marcelo; Musa, Luciano; Musso, Alfredo; Nandi, Basanta Kumar; Nania, Rosario; Nappi, Eugenio; Nattrass, Christine; Naumov, Nikolay; Navin, Sparsh; Nayak, Tapan Kumar; Nazarenko, Sergey; Nazarov, Gleb; Nedosekin, Alexander; Nicassio, Maria; Nielsen, Borge Svane; Niida, Takafumi; Nikolaev, Sergey; Nikolic, Vedran; Nikulin, Sergey; Nikulin, Vladimir; Nilsen, Bjorn Steven; Nilsson, Mads Stormo; Noferini, Francesco; Nomokonov, Petr; Nooren, Gerardus; Novitzky, Norbert; Nyanin, Alexandre; Nyatha, Anitha; Nygaard, Casper; Nystrand, Joakim Ingemar; Obayashi, Hideyuki; Ochirov, Alexander; Oeschler, Helmut Oskar; Oh, Saehanseul; Oh, Sun Kun; Oleniacz, Janusz; Oppedisano, Chiara; Ortiz Velasquez, Antonio; Ortona, Giacomo; Oskarsson, Anders Nils Erik; Otterlund, Ingvar; Otwinowski, Jacek Tomasz; Oyama, Ken; Pachmayer, Yvonne Chiara; Pachr, Milos; Padilla, Fatima; Pagano, Paola; Paic, Guy; Painke, Florian; Pajares, Carlos; Pal, S; Pal, Susanta Kumar; Palaha, Arvinder Singh; Palmeri, Armando; Papikyan, Vardanush; Pappalardo, Giuseppe; Park, Woo Jin; Passfeld, Annika; Patalakha, Dmitri Ivanovich; Paticchio, Vincenzo; Pavlinov, Alexei; Pawlak, Tomasz Jan; Peitzmann, Thomas; Pereira De Oliveira Filho, Elienos; Peresunko, Dmitri; Perez Lara, Carlos Eugenio; Perez Lezama, Edgar; Perini, Diego; Perrino, Davide; Peryt, Wiktor Stanislaw; Pesci, Alessandro; Peskov, Vladimir; Pestov, Yury; Petracek, Vojtech; Petran, Michal; Petris, Mariana; Petrov, Plamen Rumenov; Petrovici, Mihai; Petta, Catia; Piano, Stefano; Piccotti, Anna; Pikna, Miroslav; Pillot, Philippe; Pinazza, Ombretta; Pinsky, Lawrence; Pitz, Nora; Piuz, Francois; Piyarathna, Danthasinghe; Ploskon, Mateusz Andrzej; Pluta, Jan Marian; Pocheptsov, Timur; Pochybova, Sona; Podesta Lerma, Pedro Luis Manuel; Poghosyan, Martin; Polichtchouk, Boris; Pop, Amalia; Porteboeuf-Houssais, Sarah; Pospisil, Vladimir; Potukuchi, Baba; Prasad, Sidharth Kumar; Preghenella, Roberto; Prino, Francesco; Pruneau, Claude Andre; Pshenichnov, Igor; Puchagin, Sergey; Puddu, Giovanna; Pulvirenti, Alberto; Punin, Valery; Putis, Marian; Putschke, Jorn Henning; Quercigh, Emanuele; Qvigstad, Henrik; Rachevski, Alexandre; Rademakers, Alphonse; Radomski, Sylwester; Raiha, Tomi Samuli; Rak, Jan; Rakotozafindrabe, Andry Malala; Ramello, Luciano; Ramirez Reyes, Abdiel; Raniwala, Rashmi; Raniwala, Sudhir; Rasanen, Sami Sakari; Rascanu, Bogdan Theodor; Rathee, Deepika; Read, Kenneth Francis; Real, Jean-Sebastien; Redlich, Krzysztof; Reichelt, Patrick; Reicher, Martijn; Renfordt, Rainer Arno Ernst; Reolon, Anna Rita; Reshetin, Andrey; Rettig, Felix Vincenz; Revol, Jean-Pierre; Reygers, Klaus Johannes; Riccati, Lodovico; Ricci, Renato Angelo; Richter, Matthias Rudolph; Riedler, Petra; Riegler, Werner; Riggi, Francesco; Rodriguez Cahuantzi, Mario; Rohr, David; Rohrich, Dieter; Romita, Rosa; Ronchetti, Federico; Rosnet, Philippe; Rossegger, Stefan; Rossi, Andrea; Roukoutakis, Filimon; Roy, Christelle Sophie; Roy, Pradip Kumar; Rubio Montero, Antonio Juan; Rui, Rinaldo; Ryabinkin, Evgeny; Rybicki, Andrzej; Sadovsky, Sergey; Safarik, Karel; Sahu, Pradip Kumar; Saini, Jogender; Sakaguchi, Hiroaki; Sakai, Shingo; Sakata, Dosatsu; Salgado, Carlos Albert; Sambyal, Sanjeev Singh; Samsonov, Vladimir; Sanchez Castro, Xitzel; Sandor, Ladislav; Sandoval, Andres; Sano, Masato; Sano, Satoshi; Santo, Rainer; Santoro, Romualdo; Sarkamo, Juho Jaako; Scapparone, Eugenio; Scarlassara, Fernando; Scharenberg, Rolf Paul; Schiaua, Claudiu Cornel; Schicker, Rainer Martin; Schmidt, Christian Joachim; Schmidt, Hans Rudolf; Schreiner, Steffen; Schuchmann, Simone; Schukraft, Jurgen; Schutz, Yves Roland; Schwarz, Kilian Eberhard; Schweda, Kai Oliver; Scioli, Gilda; Scomparin, Enrico; Scott, Patrick Aaron; Scott, Rebecca; Segato, Gianfranco; Selioujenkov, Ilya; Senyukov, Serhiy; Seo, Jeewon; Serci, Sergio; Serradilla, Eulogio; Sevcenco, Adrian; Sgura, Irene; Shabetai, Alexandre; Shabratova, Galina; Shahoyan, Ruben; Sharma, Natasha; Sharma, Satish; Shigaki, Kenta; Shimomura, Maya; Shtejer, Katherin; Sibiriak, Yury; Siciliano, Melinda; Sicking, Eva; Siddhanta, Sabyasachi; Siemiarczuk, Teodor; Silvermyr, David Olle Rickard; Simonetti, Giuseppe; Singaraju, Rama Narayana; Singh, Ranbir; Singha, Subhash; Sinha, Bikash; Sinha, Tinku; Sitar, Branislav; Sitta, Mario; Skaali, Bernhard; Skjerdal, Kyrre; Smakal, Radek; Smirnov, Nikolai; Snellings, Raimond; Sogaard, Carsten; Soltz, Ron Ariel; Son, Hyungsuk; Song, Jihye; Song, Myunggeun; Soos, Csaba; Soramel, Francesca; Sputowska, Iwona; Spyropoulou-Stassinaki, Martha; Srivastava, Brijesh Kumar; Stachel, Johanna; Stan, Ionel; Stefanek, Grzegorz; Stefanini, Giorgio; Steinbeck, Timm Morten; Steinpreis, Matthew; Stenlund, Evert Anders; Steyn, Gideon Francois; Stocco, Diego; Stolpovskiy, Mikhail; Strabykin, Kirill; Strmen, Peter; Suaide, Alexandre Alarcon do Passo; Subieta Vasquez, Martin Alfonso; Sugitate, Toru; Suire, Christophe Pierre; Sukhorukov, Mikhail; Sultanov, Rishat; Sumbera, Michal; Susa, Tatjana; Szanto de Toledo, Alejandro; Szarka, Imrich; Szczepankiewicz, Adam; Szostak, Artur Krzysztof; Tagridis, Christos; Takahashi, Jun; Tapia Takaki, Daniel Jesus; Tauro, Arturo; Tejeda Munoz, Guillermo; Telesca, Adriana; Terrevoli, Cristina; Thader, Jochen Mathias; Thomas, Deepa; Thomas, Jim; Tieulent, Raphael Noel; Timmins, Anthony; Tlusty, David; Toia, Alberica; Torii, Hisayuki; Tosello, Flavio; Traczyk, Tomasz; Trzaska, Wladyslaw Henryk; Tsuji, Tomoya; Tumkin, Alexandr; Turrisi, Rosario; Tveter, Trine Spedstad; Ulery, Jason Glyndwr; Ullaland, Kjetil; Ulrich, Jochen; Uras, Antonio; Urban, Jozef; Urciuoli, Guido Marie; Usai, Gianluca; Vajzer, Michal; Vala, Martin; Valencia Palomo, Lizardo; Vallero, Sara; van der Kolk, Naomi; van Leeuwen, Marco; Vande Vyvre, Pierre; Vannucci, Luigi; Vargas, Aurora Diozcora; Varma, Raghava; Vasileiou, Maria; Vasiliev, Andrey; Vechernin, Vladimir; Veldhoen, Misha; Venaruzzo, Massimo; Vercellin, Ermanno; Vergara, Sergio; Vernekohl, Don Constantin; Vernet, Renaud; Verweij, Marta; Vickovic, Linda; Viesti, Giuseppe; Vikhlyantsev, Oleg; Vilakazi, Zabulon; Villalobos Baillie, Orlando; Vinogradov, Alexander; Vinogradov, Leonid; Vinogradov, Yury; Virgili, Tiziano; Viyogi, Yogendra; Vodopianov, Alexander; Voloshin, Kirill; Voloshin, Sergey; Volpe, Giacomo; von Haller, Barthelemy; Vranic, Danilo; vrebekk, Gaute; Vrlakova, Janka; Vulpescu, Bogdan; Vyushin, Alexey; Wagner, Boris; Wagner, Vladimir; Wan, Renzhuo; Wang, Dong; Wang, Mengliang; Wang, Yifei; Wang, Yaping; Watanabe, Kengo; Wessels, Johannes; Westerhoff, Uwe; Wiechula, Jens; Wikne, Jon; Wilde, Martin Rudolf; Wilk, Alexander; Wilk, Grzegorz Andrzej; Williams, Crispin; Windelband, Bernd Stefan; Xaplanteris Karampatsos, Leonidas; Yang, Hongyan; Yang, Shiming; Yano, Satoshi; Yasnopolsky, Stanislav; Yi, JunGyu; Yin, Zhongbao; Yokoyama, Hiroki; Yoo, In-Kwon; Yoon, Jongik; Yu, Weilin; Yuan, Xianbao; Yushmanov, Igor; Zach, Cenek; Zampolli, Chiara; Zaporozhets, Sergey; Zarochentsev, Andrey; Zavada, Petr; Zaviyalov, Nikolai; Zbroszczyk, Hanna Paulina; Zelnicek, Pierre; Zgura, Sorin Ion; Zhalov, Mikhail; Zhang, Xiaoming; Zhou, Daicui; Zhou, Fengchu; Zhou, You; Zhu, Xiangrong; Zichichi, Antonino; Zimmermann, Alice; Zinovjev, Gennady; Zoccarato, Yannick Denis; Zynovyev, Mykhaylo

    2012-01-01

    The ALICE experiment has measured low-mass dimuon production in pp collisions at $\\sqrt{s}$ = 7 TeV in the dimuon rapidity region 2.5<y<4. The observed dimuon mass spectrum is described as a superposition of resonance decays ($\\eta, \\rho, \\omega, \\eta^{'}, \\phi$) into muons and semi-leptonic decays of charmed mesons. The measured production cross sections for $\\omega$ and $\\phi$ are $\\sigma_\\omega (1y<4) = 5.28 $\\pm$ 0.54 (stat) $\\pm$ 0.50 (syst) mb and $\\sigma_\\phi$(1<$p_t$<5 GeV/c,2.5<y<4)=0.940 $\\pm$ 0.084 (stat) $\\pm$ 0.078 (syst) mb. The differential cross sections $d^2\\sigma/dy dp_t$ are extracted as a function of $p_t$ for $\\omega$ and $\\phi$. The ratio between the $\\rho$ and $\\omega$ cross section is obtained. Results for the $\\phi$ are compared with other measurements at the same energy and with predictions by models.

  10. Single production of X±5 /3 and Y∓4 /3 vectorlike quarks at the LHC

    Science.gov (United States)

    Chen, Chuan-Hung; Nomura, Takaaki

    2016-08-01

    Two triplet vectorlike quarks (VLQs) with hypercharges of Y =2 /3 , -1 /3 and one singlet scalar boson are embedded in the standard model to resolve the 750 GeV diphoton excess. The constraints on the tree-level Higgs- and Z -mediated flavor-changing neutral currents are discussed in detail. Besides the resolution of excess, it is found that the signal strength of diphoton Higgs decay can have a 10% deviation from the SM prediction and that the upper limits of the branching ratios for rare top-quark decays are BR (t →c (h ,Z ))processes is larger than that of a VLQ pair by QCD processes. To explore the signals of the heavy VLQs at the LHC, we thoroughly analyze the production of single X±5 /3 and Y∓4 /3 via qiqj' annihilations in p p collisions at √{s }=13 TeV . It is found that the electroweak production cross sections for d X5 /3, u Y-4 /3, and d Y4 /3 channels with mX=mY=1 TeV can be 84.3, 72.3, and 157.8 fb, respectively, and the dominant decay modes are X5 /3→(c ,t )W+ and Y-4 /3→(s ,b )W-. With adopting kinematic cuts, the significance for the p p →d W+t channel can be over 5 σ .

  11. Optimization of minoxidil microemulsions using fractional factorial design approach.

    Science.gov (United States)

    Jaipakdee, Napaphak; Limpongsa, Ekapol; Pongjanyakul, Thaned

    2016-01-01

    The objective of this study was to apply fractional factorial and multi-response optimization designs using desirability function approach for developing topical microemulsions. Minoxidil (MX) was used as a model drug. Limonene was used as an oil phase. Based on solubility, Tween 20 and caprylocaproyl polyoxyl-8 glycerides were selected as surfactants, propylene glycol and ethanol were selected as co-solvent in aqueous phase. Experiments were performed according to a two-level fractional factorial design to evaluate the effects of independent variables: Tween 20 concentration in surfactant system (X1), surfactant concentration (X2), ethanol concentration in co-solvent system (X3), limonene concentration (X4) on MX solubility (Y1), permeation flux (Y2), lag time (Y3), deposition (Y4) of MX microemulsions. It was found that Y1 increased with increasing X3 and decreasing X2, X4; whereas Y2 increased with decreasing X1, X2 and increasing X3. While Y3 was not affected by these variables, Y4 increased with decreasing X1, X2. Three regression equations were obtained and calculated for predicted values of responses Y1, Y2 and Y4. The predicted values matched experimental values reasonably well with high determination coefficient. By using optimal desirability function, optimized microemulsion demonstrating the highest MX solubility, permeation flux and skin deposition was confirmed as low level of X1, X2 and X4 but high level of X3. PMID:25318551

  12. Parameters for a Super-Flavor-Factory

    Energy Technology Data Exchange (ETDEWEB)

    Seeman, J.T.; Cai, Y.; Ecklund, S.; Novokhatski, A.; Seryi, A.; Sullivan, M.; Wienands, U.; /SLAC; Biagini, M.; Raimondi, P.; /Frascati

    2006-06-27

    A Super Flavor Factory, an asymmetric energy e{sup +}e{sup -} collider with a luminosity of order 10{sup 36} cm{sup -2} s{sup -1}, can provide a sensitive probe of new physics in the flavor sector of the Standard Model. The success of the PEP-II and KEKB asymmetric colliders in producing unprecedented luminosity above 10{sup 34} cm{sup -2} s{sup -1} has taught us about the accelerator physics of asymmetric e{sup +}e{sup -} collider in a new parameter regime. Furthermore, the success of the SLAC Linear Collider and the subsequent work on the International Linear Collider allow a new Super-Flavor collider to also incorporate linear collider techniques. This note describes the parameters of an asymmetric Flavor-Factory collider at a luminosity of order 10{sup 36} cm{sup -2} s{sup -1} at the Y(4S) resonance and about 10{sup 35} cm{sup -2} s{sup -1} at the {tau} production threshold. Such a collider would produce an integrated luminosity of about 10,000 fb{sup -1} (10 ab{sup -1}) in a running year (10{sup 7} sec) at the Y(4S) resonance. In the following note only the parameters relative to the Y(4S) resonance will be shown, the ones relative to the lower energy operations are still under study.

  13. CCL7 is a protective factor secreted by mechanically loaded osteocytes.

    Science.gov (United States)

    Kitase, Y; Lee, S; Gluhak-Heinrich, J; Johnson, M L; Harris, S E; Bonewald, L F

    2014-11-01

    In a search for factors up-regulated by mechanical strain in osteocytes, we discovered that chemokine (C-C motif) ligand 7 (CCL7), a chemotactic myokine, was highly expressed in MLO-Y4 osteocyte-like cells. Although MLO-Y4 cells secrete potent chemotactic factors for osteoclast precursors, CCL7 was not responsible for this activity. CCL7 was increased in osteocytes in response to tooth movement in vivo. Since mechanical loading plays a crucial role in maintaining osteocyte viability, CCL7 was tested for protective activity and found to be protective against cell death induced by dexamethasone and etoposide. CCL7 specific antibody partially, but in combination with indomethacin, completely abrogated the protective effects of fluid flow shear stress against dexamethasone-induced cell death. CCL7 activated the β-catenin pathway through phosphorylation of glycogen synthase kinase 3 (GSK-3), suggesting that this pathway is responsible for the observed protective effects. A related cytokine, CCL2, also produced by MLO-Y4 cells but not regulated by mechanical loading, proved to be more potent and protected against cell death induced by not only dexamethasone, but also by Tumor Necrosis Factor α (TNFα). Whereas osteocytes may produce CCL2 in constitutively low levels, a major function of mechanically induced CCL7 may be to selectively protect osteocytes in an autocrine manner against glucocorticoid-induced cell death. PMID:25274752

  14. Serotonin acts as a novel regulator of interleukin-6 secretion in osteocytes through the activation of the 5-HT(2B) receptor and the ERK1/2 signalling pathway.

    Science.gov (United States)

    Li, Xianxian; Ma, Yuanyuan; Wu, Xiangnan; Hao, Zhichao; Yin, Jian; Shen, Jiefei; Li, Xiaoyu; Zhang, Ping; Wang, Hang

    2013-11-29

    Interleukin-6 (IL-6) is a potent stimulator of osteoclastic bone resorption. Osteocyte secretion of IL-6 plays an important role in bone metabolism. Serotonin (5-HT) has recently been reported to regulate bone metabolism. The aim of this study was to evaluate the effect of serotonin on osteocyte expression of IL-6. The requirement for the 5-HT receptor(s) and the role of the extracellular signal-regulated kinase 1/2 (ERK1/2) in serotonin-induced IL-6 synthesis were examined. In this study, real-time PCR and ELISA were used to analyse IL-6 gene and protein expression in serotonin-stimulated MLO-Y4 cells. ERK1/2 pathway activation was determined by Western blot. We found that serotonin significantly activated the ERK1/2 pathway and induced IL-6 mRNA expression and protein synthesis in cultured MLO-Y4 cells. However, these effects were abolished by pre-treatment of MLO-Y4 cells with a 5-HT2B receptor antagonist, RS127445 or the ERK1/2 inhibitor, PD98059. Our results indicate that serotonin stimulates osteocyte secretion of IL-6 and that this effect is associated with activation of 5-HT2B receptor and the ERK1/2 pathway. These findings provide support for a role of serotonin in bone metabolism by indicating serotonin regulates bone remodelling by mediating an inflammatory cytokine. PMID:24211588

  15. Unfractionated Heparin Promotes Osteoclast Formation in Vitro by Inhibiting Osteoprotegerin Activity

    Directory of Open Access Journals (Sweden)

    Binghan Li

    2016-04-01

    Full Text Available Heparin has been proven to enhance bone resorption and induce bone loss. Since osteoclasts play a pivotal role in bone resorption, the effect of heparin on osteoclastogenesis needs to be clarified. Since osteocytes are the key modulator during osteoclastogenesis, we evaluated heparin’s effect on osteoclastogenesis in vitro by co-culturing an osteocyte cell line (MLO-Y4 and pre-osteoclasts (RAW264.7. In this co-culture system, heparin enhanced osteoclastogenesis and osteoclastic bone resorption while having no influence on the production of RANKL (receptor activator of NFκB ligand, M-CSF (macrophage colony-stimulating factor, and OPG (osteoprotegerin, which are three main regulatory factors derived from osteocytes. According to previous studies, heparin could bind specifically to OPG and inhibit its activity, so we hypothesized that this might be a possible mechanism of heparin activity. To test this hypothesis, osteoclastogenesis was induced using recombinant RANKL or MLO-Y4 supernatant. We found that heparin has no effect on RANKL-induced osteoclastogenesis (contains no OPG. However, after incubation with OPG, the capacity of MLO-Y4 supernatant for supporting osteoclast formation was increased. This effect disappeared after OPG was neutralized and reappeared after OPG was replenished. These results strongly suggest that heparin promotes osteocyte-modulated osteoclastogenesis in vitro, at least partially, through inhibiting OPG activity.

  16. Unfractionated Heparin Promotes Osteoclast Formation in Vitro by Inhibiting Osteoprotegerin Activity.

    Science.gov (United States)

    Li, Binghan; Lu, Dan; Chen, Yuqing; Zhao, Minghui; Zuo, Li

    2016-01-01

    Heparin has been proven to enhance bone resorption and induce bone loss. Since osteoclasts play a pivotal role in bone resorption, the effect of heparin on osteoclastogenesis needs to be clarified. Since osteocytes are the key modulator during osteoclastogenesis, we evaluated heparin's effect on osteoclastogenesis in vitro by co-culturing an osteocyte cell line (MLO-Y4) and pre-osteoclasts (RAW264.7). In this co-culture system, heparin enhanced osteoclastogenesis and osteoclastic bone resorption while having no influence on the production of RANKL (receptor activator of NFκB ligand), M-CSF (macrophage colony-stimulating factor), and OPG (osteoprotegerin), which are three main regulatory factors derived from osteocytes. According to previous studies, heparin could bind specifically to OPG and inhibit its activity, so we hypothesized that this might be a possible mechanism of heparin activity. To test this hypothesis, osteoclastogenesis was induced using recombinant RANKL or MLO-Y4 supernatant. We found that heparin has no effect on RANKL-induced osteoclastogenesis (contains no OPG). However, after incubation with OPG, the capacity of MLO-Y4 supernatant for supporting osteoclast formation was increased. This effect disappeared after OPG was neutralized and reappeared after OPG was replenished. These results strongly suggest that heparin promotes osteocyte-modulated osteoclastogenesis in vitro, at least partially, through inhibiting OPG activity. PMID:27110777

  17. Oral cancer knowledge, behavior, and attitude among osteopathic medical students.

    Science.gov (United States)

    McCready, Zachary R; Kanjirath, Preetha; Jham, Bruno C

    2015-06-01

    Approximately 21,000 osteopathic medical students were enrolled in the USA in 2012-2013. These future physicians are being educated with an emphasis on a holistic or patient-centered approach, with a focus on preventive care. Considering the importance of preventive care and early diagnosis in the outcomes of oral malignancies, our goal in this study was to assess the knowledge, behavior, and attitude of osteopathic medical students in relation to oral cancer. To this end, 204 second-year (Y2) and 194 fourth-year (Y4) medical students were invited to participate in an electronic survey. Forty-one Y2 and 44 Y4 students agreed to participate (20 and 22% response rate, respectively). The results showed that most Y2 and Y4 students were knowledgeable in certain areas (demographic features, important risk factors, and histologic feature), but deficient in others (clinical presentation, association of human papillomavirus (HPV) with oropharyngeal cancers, and screening recommendations). Head, neck, and oral examination habits were reported as being performed occasionally. Overall, students reported feeling uninformed about oral cancer and showed an interest in receiving further education on the subject. Our findings confirm that an overall improvement in oral cancer education in the medical curriculum is needed. Interprofessional collaboration between dental and medical schools may prove to be a valid approach to achieve this goal, which may possibly lead to increased detection of early oral cancerous lesions and, ultimately, improved mortality rates. PMID:24882439

  18. HACEK infective endocarditis: characteristics and outcomes from a large, multi-national cohort.

    Directory of Open Access Journals (Sweden)

    Stephen T Chambers

    Full Text Available The HACEK organisms (Haemophilus species, Aggregatibacter species, Cardiobacterium hominis, Eikenella corrodens, and Kingella species are rare causes of infective endocarditis (IE. The objective of this study is to describe the clinical characteristics and outcomes of patients with HACEK endocarditis (HE in a large multi-national cohort. Patients hospitalized with definite or possible infective endocarditis by the International Collaboration on Endocarditis Prospective Cohort Study in 64 hospitals from 28 countries were included and characteristics of HE patients compared with IE due to other pathogens. Of 5591 patients enrolled, 77 (1.4% had HE. HE was associated with a younger age (47 vs. 61 years; p<0.001, a higher prevalence of immunologic/vascular manifestations (32% vs. 20%; p<0.008 and stroke (25% vs. 17% p = 0.05 but a lower prevalence of congestive heart failure (15% vs. 30%; p = 0.004, death in-hospital (4% vs. 18%; p = 0.001 or after 1 year follow-up (6% vs. 20%; p = 0.01 than IE due to other pathogens (n = 5514. On multivariable analysis, stroke was associated with mitral valve vegetations (OR 3.60; CI 1.34-9.65; p<0.01 and younger age (OR 0.62; CI 0.49-0.90; p<0.01. The overall outcome of HE was excellent with the in-hospital mortality (4% significantly better than for non-HE (18%; p<0.001. Prosthetic valve endocarditis was more common in HE (35% than non-HE (24%. The outcome of prosthetic valve and native valve HE was excellent whether treated medically or with surgery. Current treatment is very successful for the management of both native valve prosthetic valve HE but further studies are needed to determine why HE has a predilection for younger people and to cause stroke. The small number of patients and observational design limit inferences on treatment strategies. Self selection of study sites limits epidemiological inferences.

  19. L-arginine destabilizes oral multi-species biofilm communities developed in human saliva.

    Science.gov (United States)

    Kolderman, Ethan; Bettampadi, Deepti; Samarian, Derek; Dowd, Scot E; Foxman, Betsy; Jakubovics, Nicholas S; Rickard, Alexander H

    2015-01-01

    The amino acid L-arginine inhibits bacterial coaggregation, is involved in cell-cell signaling, and alters bacterial metabolism in a broad range of species present in the human oral cavity. Given the range of effects of L-arginine on bacteria, we hypothesized that L-arginine might alter multi-species oral biofilm development and cause developed multi-species biofilms to disassemble. Because of these potential biofilm-destabilizing effects, we also hypothesized that L-arginine might enhance the efficacy of antimicrobials that normally cannot rapidly penetrate biofilms. A static microplate biofilm system and a controlled-flow microfluidic system were used to develop multi-species oral biofilms derived from pooled unfiltered cell-containing saliva (CCS) in pooled filter-sterilized cell-free saliva (CFS) at 37° C. The addition of pH neutral L-arginine monohydrochloride (LAHCl) to CFS was found to exert negligible antimicrobial effects but significantly altered biofilm architecture in a concentration-dependent manner. Under controlled flow, the biovolume of biofilms (μm(3)/μm(2)) developed in saliva containing 100-500 mM LAHCl were up to two orders of magnitude less than when developed without LAHCI. Culture-independent community analysis demonstrated that 500 mM LAHCl substantially altered biofilm species composition: the proportion of Streptococcus and Veillonella species increased and the proportion of Gram-negative bacteria such as Neisseria and Aggregatibacter species was reduced. Adding LAHCl to pre-formed biofilms also reduced biovolume, presumably by altering cell-cell interactions and causing cell detachment. Furthermore, supplementing 0.01% cetylpyridinium chloride (CPC), an antimicrobial commonly used for the treatment of dental plaque, with 500 mM LAHCl resulted in greater penetration of CPC into the biofilms and significantly greater killing compared to a non-supplemented 0.01% CPC solution. Collectively, this work demonstrates that LAHCl moderates multi

  20. Doença periodontal em portadoras de valvopatia durante a gravidez: estudo clínico e microbiológico Enfermedad periodontal en portadoras de valvopatía durante la gravidez: estudio clínico y microbiológico Periodontal disease in pregnant patients with rheumatic valvular disease: clinical and microbiological study

    Directory of Open Access Journals (Sweden)

    Walkiria Samuel Ávila

    2011-04-01

    Full Text Available FUNDAMENTO: A doença periodontal representa risco à gestante portadora de valvopatia reumática, seja para contrair endocardite infecciosa, seja por propiciar complicações obstétricas. OBJETIVO: Estudar a frequência da doença periodontal em portadoras de valvopatia reumática durante a gravidez. MÉTODOS: Foram estudadas 140 gestantes, comparáveis quanto a idade e o nível socioeconômico, divididas em: 70 portadoras de doença valvar reumática e 70 mulheres saudáveis. Todas se submeteram a: 1 avaliação clínica odontológica que incluiu a análise dos seguintes parâmetros: 1.1 profundidade à sondagem, 1.2 distância da linha esmalte-cemento à margem gengival, 1.3 nível clínico de inserção, 1.4 índice de sangramento, 1.5 índice de placa bacteriana, e, 1.6 comprometimento de furca; e, 2 exame microbiológico nas amostras de saliva e do cone que considerou o controle positivo para as cepas das bactérias Porphyromonas gingivalis, Tannerella forsithia e Aggregobacter actinomycetemcomitans. RESULTADOS: A lesão valvar mitral foi prevalente (65 casos = 92,8% dentre as gestantes cardiopatas. A comparação entre os grupos mostrou não haver diferenças entre idade e a paridade, e embora tenham sido verificadas diferenças entre as medidas da distância da linha esmalte-cemento à margem gengival (p = 0,01 e o índice de placa (p=0,04, a frequência da doença periodontal identificada em 20 (14,3% gestantes, não foi diferente entre os grupos (p = 0,147. O exame microbiológico mostrou uma proporção maior da bactéria P. gingivalis na saliva de gestantes saudáveis (p = 0,004. CONCLUSÃO: O estudo clínico e microbiológico periodontal durante a gravidez demonstrou igual frequência da doença periodontal em portadoras de valvopatia reumática quando comparada às mulheres saudáveis.FUNDAMENTO: La enfermedad periodontal, caracterizada por el estado inflamatorio e infeccioso permanente de la cavidad oral, representa riesgo a la

  1. Fumigation Activity of AITC against Three Stored-grain Insects%辣根素对3种仓储害虫的熏蒸作用

    Institute of Scientific and Technical Information of China (English)

    彭贝; 王振华; 靳勇; 蔡万伦; 华红霞; 杨长举; 高艮亮

    2011-01-01

    AITC was tested for its insecticidal activities against three stored - grain insects by the method of triangular bottle obturation fumigant at 28 ℃.After 48 h of exposure, good insecticidal activity against adults of Tribolium castaneum ( H.), Rhizopertha dominica ( F.) and Sitophilus zeamais ( M.) was achieved with AITCI and AITCⅡ.And AITCⅡ was more toxic.The mortality regression equation and LC50 value of AITCI against adults of Tribolium castaneum, Rhizopertha dominica and Sitophilus zeamais was Y = - 3.94 + 4.77X, 6.68 μL/L; Y = - 4.73 +11.11X, 2.67 μL/L; Y = -4.22 +7.47X, 3.68 μL/L, respectively.The mortality regression equation and the LC50 value of AITCII against adults of Tribolium castaneum, Rhizopertha dominica and Sitophilus zeamais was Y =-4.17 +5.65X, 5.47 μL/L; Y= -1.82 +5.39X, 1.95 μL/L; Y= -2.90 +6.04X, 3.02μL/L, respectively.%在28℃环境温度下,利用三角瓶熏蒸法,测定了辣根素对3种仓储害虫的熏蒸毒力.结果表明,熏蒸48 h后,辣根素Ⅰ和辣根素Ⅱ对赤拟谷盗、谷蠹和玉米象成虫均具有明显的熏蒸效果.辣根素Ⅱ对3种仓储害虫的熏蒸效果均更好.辣根素Ⅰ熏蒸处理48 h后,对赤拟谷盗、谷蠹、玉米象成虫的毒力回归方程和LC50分别为:赤拟谷盗y=-3.94+4.77X,6.68μL/L;谷蠹y=-4.73+11.11X,2.67μL/L;玉米象y=-4.22+7.47X,3.68μL/L.辣根素Ⅱ熏蒸处理48 h后,对赤拟谷盗、谷蠹、玉米象成虫的毒力回归方程和LC50分别为:赤拟谷盗y=-4.17+5.65X,5.47μL/L;谷蠹y=-1.82+5.39X,1.95μL/L;玉米象y=-2.90+6.04X,3.02μL/L.

  2. The effects of substrate-streching strain on the BMP-2 mRNA expression in three kinds of mouse cell lines%基底拉伸应变对小鼠三种骨组织细胞BMP-2 mRNA表达的影响

    Institute of Scientific and Technical Information of China (English)

    闫玉仙; 宋梅; 郭春; 郭勇; 宫元伟; 李瑞欣; 张西正

    2010-01-01

    目的 研究基底拉伸应变对小鼠成骨细胞系MC3T3-E1、破骨细胞系RAW264.7及骨细胞MLO-Y4三种细胞BMP-2 mRNA表达的影响.方法 三种细胞随机分为0 με、1 000 με、1 500 με、2 000 με、2 500 με和5 000 με组,最佳拉伸时间和周期为1次/d,每次1 h,连续3 d,频率为0.5 Hz.采用卫生装备研究所自行设计研制的四点弯曲装置对小鼠三种细胞进行拉伸加载.采用RT-PCR技术分别研究不同应变对小鼠三种细胞BMP-2 mRNA表达.结果 MC3T3-E1细胞RT-PCR结果显示:1 500 με、2 000 με组和2 500 με组与0 με组相比BMP-2 mRNA表达显著增强(P<0.01);5 000 με组与0 με组相比BMP-2 mRNA表达显著降低(P<0.01);RAW264.7细胞RT-PCR结果显示:1 500 με、2 000 με组和2 500 με组与0 με组相比BMP-2 mRNA表达显著降低(P<0.01);5 000με组与0 με组相比BMP-2 mRNA表达显著降低(P<0.01);MLO-Y4细胞BMP2基因表达结果与MC3T3-E1一致.结论 ①BMP-2在成骨细胞系MC3T3-E1、破骨细胞系RAW-264.7及骨细胞系MLO-Y4三种细胞中均有表达;②1 500 με、2 000 με、2 500 με三种生理剂量的拉伸应变可以显著增加MC3T3-E1、MLO-Y4 细胞BMP-2的表达,并呈剂量依赖性,超生理剂量5 000 με可以显著降低MC3T3-E1、MLO-Y4细胞BMP-2的表达;③相同的力学拉伸作用条件下,BMP-2在RAW-264.7细胞中表达与MC3T3-E1、MLO-Y4细胞的表达趋势相反.

  3. Effect of the probiotic Lactobacilli reuteri (Prodentis in the management of periodontal disease: a preliminary randomized clinical trial

    Directory of Open Access Journals (Sweden)

    M.R. Vivekananda

    2010-11-01

    Full Text Available Objectives: The aim of this study was to evaluate the effects of Lactobacilli reuteri (Prodentis alone and in combination with scaling and root planing (SRP in a double blind, randomized, placebo-controlled clinical trial of volunteers with chronic periodontitis. Methods: Thirty, otherwise systemically healthy, chronic periodontitis patients (19 males and 11 females, aged between 34 and 50 years were included. The study period was 42 days. ‘Split-mouth’ design was used for the SRP, which was performed on day 0; two quadrants (either right or left were treated with SRP whereas the remaining two quadrants were left untreated. The participants received a toothbrush, toothpaste, and brushing instructions. L. reuteri Prodentis lozenges (1×108 CFU DSM17938 + 1×108 CFU ATCC PTA 5289 or the corresponding placebo lozenges were taken twice daily from day 21 to day 42. Statistical analysis was done for comparisons of clinical parameters (Plaque Index (PI, Gingival Index (GI, Gingival Bleeding Index (GBI, probing pocket depth (PPD, clinical attachment level (CAL and microbiological levels of the pathogens Aggregibacter actinomycetemcomitans (Aa, Porphyromonas gingivalis (Pg, and Prevotella intermedia (Pi. All p-values less than 0.05 were considered significant. Assessments were made on day 0 before SRP treatment, on day 21 before administration of the lozenges, and on day 42. Results: At day 42, the PI, GI, and GBI were significantly reduced by all treatment modalities. When ranked, the amount of PI, GI and GBI reduction by the different treatments was SRP + Prodentis > Prodentis > SRP + placebo > placebo; all differences were statistically significant. For PPD and CAL, the best result was obtained with the SRP + Prodentis treatment. PPD was reduced from 5.08±0.75 to 3.78±0.61 mm (p<0.001 and CAL from 3.93±0.93 to 2.85±0.74 mm (p<0.001. Prodentis, either alone or following SRP, reduced Aa, Pi, and Pg by 1 log10 unit

  4. Detection of periodontal pathogens in the patients with aortic aneurysm

    Institute of Scientific and Technical Information of China (English)

    Ding Fang; Lyu Yalin; Han Xiao; Zhang Hai; Liu Dongyu; Hei Wei; Liu Yinhua

    2014-01-01

    Background The occurrence and development of aortic aneurysm (AA) are associated with infection.Some researchers have detected the DNA of periodontal pathogens in AA samples in certain populations.However,it has not been done in Chinese population.The objective of this study was to evaluate the prevalence of periodontal pathogens in oral tissue samples and aneurysm samples of AA patients.Methods Eighty-nine subjects with AA and 59 subjects without AA were examined.Periodontal clinical parameters were evaluated.Unstimulated saliva and subgingival plaque somples were collected from all subjects.Twenty-six dissected AA samples were obtained.Evidence of eight periodontal pathogens including Porphyromonas gingivalis (Pg),Actinobacillus actinomycetemcomitans (Aa),Prevotella intermedia (Pi),Tannerella forsythensis (Tf),Treponema denticola (Td),Campylobacter rectus (Cr),Fusobacterium nucleatum (Fn),and Prevotella nigrescens (Pn) was ascertained in all samples by 16S rRNA-based polymerase chain reaction (PCR) assay.Results The periodontal indexes including plaque index (PLI),probing depth (PD),bleeding index (BI),and clinical attachment loss (CAL),of the six Ramfjord index teeth were significantly higher in the AA group than those in the control group (P <0.01).Eight periodontal pathogens in subgingival plaque samples were more frequently detected in the AA group than in control group.The difference in prevalence between the groups was significant for six (out of eight) periodontal pathogens assayed (Pg,Pi,Fn,Pn,Tf,and Td,P <0.01).Additionally,all eight periodontal pathogens were more frequently detected in saliva samples of the AA group than in those of the control group,again with six (out of eight) (Pg,Pi,Fn,Cr,Tf,and Td) displaying significant differences in prevalence between the two groups (P <0.01).Out of 26 aneurysm samples examined,Pg,Pi,Fn,Crand Tfwere detected in 6 (23.1%),2 (7.7%),3 (11.5%),1 (3.8%),2 (7.7%),respectively,and Aa,Pn,and Td were not

  5. Relationship between herpesviruses and periodontal pathogenic bacteria in subgingival plaque%侵袭性牙周炎患者龈下菌斑中的疱疹病毒与牙周致病菌

    Institute of Scientific and Technical Information of China (English)

    丁芳; 冯向辉; 孟焕新; 赵亦兵; 张立; 路瑞芳; 陈智滨

    2008-01-01

    目的:检测侵袭性牙周炎(aggressive periodontitis, AgP)患者龈下菌斑中的人巨细胞病毒(human cytomegalovirus, HCMV)、EB病毒1型(Epstein-Barr virus.1,EBV一1),探讨疱疹病毒与牙周致病菌及牙周临床指标间的关系.方法:应用巢式PCR技术,检测89名AgP患者和31名健康对照者龈下菌斑中的2种疱疹病毒:人巨细胞病毒(HCMV)、EB病毒1型(EBV-1),同时应用16S rRNA为基础的PCR技术检测8种牙周可疑致病菌,包括伴放线放线杆菌(Actinobacillus actinomycetemcomitans, Aa)、牙龈卟啉单胞菌(Porphyromonas gingivalis, Pg)、福赛类杆菌(Bacteroides forsythus, Bf;现已更名为Tannerella forsythensis, Tf)、中间普氏菌(Prevotella intermedia, Pi)、直形弯曲菌(Campylobacter rectus, Cr)、具核梭杆菌(Fusobacterium nucleatum, Fn)、牙密螺旋体(Treponema denticola, Td)、变黑普氏菌(Prevotella nigrescens, Pn).结果:89名AgP患者的HCMV检出率为43.8%,显著高于健康对照者(12.9%),差异有统计学意义(P0.05).检出6~8种牙周致病菌的AgP患者其HCMV和EBV-1检出率均明显高于仅检出3~5种细菌的AgP患者的病毒检出率(P<0.05).结论:HCMV、EBV-1在AgP患者龈下的检出率较健康对照高;且在检出牙周致病菌种类数较多的样本中,疱疹病毒的检出率高,提示牙周感染HCMV和EBV-1与侵袭性牙周炎相关,疱疹病毒与牙周致病菌在侵袭性牙周炎的发病中可能起着协同作用.

  6. 6种细菌在不同类型牙周病患者龈下菌斑中检出率的比较%Comparative analysis of 6 kinds of bacteria in the subgingival plaque in different types of patients with periodontal diseases

    Institute of Scientific and Technical Information of China (English)

    马英英; 张韬文; 姜雨汐; 刘树泰

    2015-01-01

    目的:检测伴放线放线杆菌似ctinobacillus actinomycetemcomitans,Aa)、牙龈卟啉单胞菌(Porp yr omonas gingivalis,Pg)、福赛斯坦纳菌(Tannerella forsythia,Tf)、直肠弯曲杆菌(Campylobacter rectus,Cr)、侵蚀艾肯菌(Eikenella corrodens ,Ec)、产黑普氏菌(Prevotella nigrescens,Pn)在牙周健康者、菌斑性牙龈病患者及中重度慢性牙周炎患者龈下菌斑中的检出率,研究6种细菌与牙周病的关系.方法:选取40例牙周健康者(A组)、40例菌斑性牙龈病者(B组)和40例中重度慢性牙周炎患者(C组),收集龈下菌斑样本,提取细菌DNA,以16SrRNA为基础的特异性引物进行多重PCR扩增技术检测,记录上述6种细菌的检出率.应用SPSS13.0软件包对数据进行x2检验.结果:A、B组之间,Pn、Cr、Pg3种细菌的检出率存在显著差异(P<0.05);B、C组之间,Ec、Cr、Pg、Tf、Aa5种细菌的检出率存在显著差异(P<0.05);A、C组之间,Ec、Pn、Cr、Pg、Tf、Aa6种细菌检出率存在显著差异(P<0.05).结论:Ec、Pn、Cr、Pg、Tf在中重度慢性牙周炎患者中检出率明显高于健康者,说明其与中重度牙周炎具有显著相关性.Ec、Cr、Pg、Tf4种细菌在中重度慢性牙周炎中检出率显著高于菌斑性牙龈病患者,说明其与牙周炎的进展程度相关.

  7. 慢性牙周炎患者的龈下微生物与吸烟状况%Subgingival microflora and smoking status in chronic periodontitis

    Institute of Scientific and Technical Information of China (English)

    徐莉; B·G·Loos; 等

    2002-01-01

    目的评价慢性牙周炎患者的吸烟状况与龈下牙周致病微生物的百分比.方法 112例慢性牙周炎患者,根据吸烟状况分为:①重度吸烟组>10支/天(n=32);②轻度吸烟组≤10支/天(n=18);③戒烟者组(n=24);④非吸烟组(n=38).观察者口内每个象限,选取探诊最深的1或2个位点, 纸捻法取龈下菌斑,厌氧培养;并测量该位点的探诊深度(Probe Depth, PD)、附着丧失(Attachment Loss,AL)和探诊后出血(Bleeding of probe,BOP).结果①取样位点临床指标的均值为PD:6.3mm、AL:6.5mm及BOP:89%,各组间无显著性差异.②组间菌落形成单位和龈下微生物伴放线放线杆菌(Actinobacillus actinomycetemcomitans,A.a)、牙龈卟啉单胞菌(Porphyromanas gingivalis,P.g)、中间普氏菌(Prevotella intermedia,P.i)、核梭杆菌(Fusobacterium nucleatum,F.n) 和微消化球菌(Peptostreptococcus micros,P.m)的百分比均无差异.③方差分析显示仅轻度吸烟组福赛类杆菌(Bacteroides forsythus,B.f)的百分比略高于其它组(P<0.04).结论慢性牙周炎患者,探诊深度和附着丧失相似的位点,吸烟组、戒烟组和非吸烟组的龈下牙周致病微生物百分比(B.f除外)无显著差异.

  8. Potential role of purinergic signaling in lithium-induced nephrogenic diabetes insipidus.

    Science.gov (United States)

    Zhang, Yue; Nelson, Raoul D; Carlson, Noel G; Kamerath, Craig D; Kohan, Donald E; Kishore, Bellamkonda K

    2009-05-01

    Lithium (Li)-induced nephrogenic diabetes insipidus (NDI) has been attributed to the increased production of renal prostaglandin (PG)E(2). Previously we reported that extracellular nucleotides (ATP/UTP), acting through P(2y2) receptor in rat medullary collecting duct (mCD), produce and release PGE(2). Hence we hypothesized that increased production of PGE(2) in Li-induced NDI may be mediated by enhanced purinergic signaling in the mCD. Sprague-Dawley rats were fed either control or Li-added diet for 14 or 21 days. Li feeding resulted in marked polyuria and polydipsia associated with a decrease in aquaporin (AQP)2 protein abundance in inner medulla ( approximately 20% of controls) and a twofold increase in urinary PGE(2). When acutely challenged ex vivo with adenosine 5'-O-(3-thiotriphosphate) (ATPgammaS), UTP, or ADP, mCD of Li-fed rats showed significantly higher increases (50-130% over control diet-fed rats) in PGE(2) production, indicating that more than one subtype of P(2y) receptor is involved. This was associated with a 3.4-fold increase in P(2y4), but not P(2y2), receptor mRNA expression in the inner medulla of Li-fed rats compared with control diet-fed rats. Confocal laser immunofluorescence microscopy revealed predominant localization of both P(2y2) and P(2y4) receptors in the mCD of control or Li diet-fed rats. Together, these data indicate that in Li-induced NDI 1) purinergic signaling in the mCD is sensitized with increased production of PGE(2) and 2) P(2y2) and/or P(2y4) receptors may be involved in the enhanced purinergic signaling. Our study also reveals the potential beneficial effects of P(2y) receptor antagonists in the treatment and/or prevention of Li-induced NDI.

  9. Expression of embryonic hemoglobin genes in mice heterozygous for α-thalassemia or β-duplication traits and in mice heterozygous for both traits

    International Nuclear Information System (INIS)

    Hemoglobins of mouse embryos at 11.5 through 16.5 days of gestation were separated by electrophoresis on cellulose acetate and quantitated by a scanning densitometer to study the effects of two radiation-induced mutations on the expression of embryonic hemoglobin genes in mice. Normal mice produce three kinds of embryonic hemoglobins. In heterozygous α-thalassemic embryos, expression of EI (x2y2) and EII (α2y2) is deficient because the x- and α-globin genes of one of the allelic pairs of Hba on chromosome 11 was deleted or otherwise inactivated by X irradiation. Simultaneous inactivation of the x- and α-globin genes indicates that these genes must be closely linked. Reduced x- and α-chain synthesis results in an excess of y chains that associate as homotetramers. This unique y4 hemoglobin also appears in β-duplication embryos where excess y chains are produced by the presence of three rather than two functional alleles of y- and β-globin genes. In double heterozygotes, which have a single functional allele of x- and α-globin genes and three functional alleles of y- and β-globin genes, synthesis of α and non-α chains is severely imbalanced and half of the total hemoglobin is y4. Mouse y4 has a high affinity for oxygen, P50 of less than 10 mm Hg, but it lacks cooperativity so is inefficient for oxygen transport. The death of double heterozygotes in late fetal or neonatal life may be in large part to oxygen deprivation to the tissues

  10. Characterization of the Key Aroma Volatile Compounds in Cranberry (Vaccinium macrocarpon Ait.) Using Gas Chromatography-Olfactometry (GC-O) and Odor Activity Value (OAV).

    Science.gov (United States)

    Zhu, JianCai; Chen, Feng; Wang, LingYing; Niu, YunWei; Chen, HeXing; Wang, HongLin; Xiao, ZuoBing

    2016-06-22

    The volatile compounds of cranberries obtained from four cultivars (Early Black, Y1; Howes, Y2; Searles, Y3; and McFarlin, Y4) were analyzed by gas chromatography-olfactometry (GC-O), gas chromatography-mass spectrometry (GC-MS), and GC-flame photometric detection (FPD). The result presented that a total of thirty-three, thirty-four, thirty-four, and thirty-six odor-active compounds were identified by GC-O in the Y1, Y2, Y3, and Y4, respectively. In addition, twenty-two, twenty-two, thirty, and twenty-seven quantified compounds were demonstrated as important odorants according to odor activity values (OAVs > 1). Among these compounds, hexanal (OAV: 27-60), pentanal (OAV: 31-51), (E)-2-heptenal (OAV: 17-66), (E)-2-hexenal (OAV: 18-63), (E)-2-octenal (OAV: 10-28), (E)-2-nonenal (OAV: 8-77), ethyl 2-methylbutyrate (OAV: 10-33), β-ionone (OAV: 8-73), 2-methylbutyric acid (OAV: 18-37), and octanal (OAV: 4-24) contributed greatly to the aroma of cranberry. Partial least-squares regression (PLSR) was used to process the mean data accumulated from sensory evaluation by the panelists, odor-active aroma compounds (OAVs > 1), and samples. Sample Y3 was highly correlated with the sensory descriptors "floral" and "fruity". Sample Y4 was greatly related to the sensory descriptors "mellow" and "green and grass". Finally, an aroma reconstitution (Model A) was prepared by mixing the odor-active aroma compounds (OAVs > 1) based on their measured concentrations in the Y1 sample, indicating that the aroma profile of the reconstitution was pretty similar to that of the original sample. PMID:27265519

  11. Diversidad, distribución de la comunidad de insectos acuáticos y calidad del agua de la parte alta-media del río David, provincia de Chiriquí, república de Panamá

    Directory of Open Access Journals (Sweden)

    Pino Selles Roberto

    2011-11-01

    Full Text Available Se determinó la calidad del agua, la diversidad y distribución de los insectos acuáticos en la parte alta­media del río David, Chiriquí, Panamá, de enero a mayo de 2005. Las muestras se recolectaron con pinzas entomológicas para obtener los insectos refugiados bajo rocas y en hojarasca, y una red triangular (D­ net para capturar los insectos nadadores en la superficie y en la columna de agua, barridos de fondo y la vegetación marginal sumergida. Los especímenes se colocaron en alcohol al 70% con unas gotas de glicerina. La comunidad de insectos acuáticos consistió de 91 géneros agrupados en 48 familias, pertenecientes a 9 órdenes de la Clase Insecta. El Índice de Diversidad de Shannon­ Weaver fue de 2.75, lo que demuestra una alta diversidad en este ecosistema. Los géneros Eurygerris sp. y Rhagovelia sp. fueron los más abundantes. El Índice de Similitud de Jaccard mostró la mayor similitud entre las estaciones 3 y 4 con un 53.7%, y la menor entre las estaciones 2 y 4, con un 34.7% de similitud. El índice biotico BMWP/Col. presentó valores de 189, 245, 245 y 190 en la estaciones 1, 2, 3 y 4, respectivamente. Estos valores lo ubican dentro de la Clase de agua I, e indica que las aguas son de buena calidad y que están libres de contaminación o poco alteradas.

  12. Induction of DNA-strand breaks after X- irradiation in murine bone cells of various differentiation capacities

    Science.gov (United States)

    Lau, P.; Hellweg, C. E.; Kirchner, S.; Arenz, A.; Baumstark-Khan, C.; Horneck, G.

    Bone loss resulting from long-duration space flight is a well known medical risk for space travellers, as a weakened skeleton is more susceptible to bone fractures. In addition to weightlessness the astronaut is also exposed to cosmic ionizing radiation. In order to elucidate changes in bone cell metabolism by ionizing radiation, a ground-based bone cell model has been developed. This model consists of a bunch of immortalized murine osteocyte, osteoblast and pre-osteoblast cell lines representing discrete stages of differentiation: The osteocyte cell line MLO-Y4 (obtained from L. Bonewald, Kansas City, USA), the osteoblast cell line OCT-1 (obtained from D. Chen, San Antonio, USA), and the subclones 4 and 24 of the osteoblast cell line MC3T3-E1 (obtained from ATCC, Manassas, Virginia, USA). Regarding their growth properties, MLO-Y4 cells show the highest growth velocity with a doubling time of 15.8 h. The osteoblast cell line OCT-1 has a doubling time of 27.3 h. The respective values for MC3T3-E1 subclone 24 and S4 are 90.5 h and 51.6 h. To investigate the stage of differentiation, the expression of alkaline phosphatase, of osteocalcin and of E11 was examined. Survival after X-ray exposure was determined using the colony forming ability test. The resulting dose-effect relationships revealed significant differences. The parameter D0 of the survival curves ranges between 1.8 Gy for OCT-1, 1.9 Gy for MLO-Y4, 2.0 Gy for subclone 24 and 2,3 Gy for subclone 4. The quantitative acquisition of DNA-strand breaks was performed by Fluorescent Analysis of DNA-Unwinding (FADU). The results can be correlated with the corresponding survival curve. In conclusion, the cell lines with higher differentiation levels are less sensitive to radiation when compared to the lower differentiated osteoblast cell lines.

  13. Molecular analysis of central feeding regulation by neuropeptide Y (NPY) neurons with NPY receptor small interfering RNAs (siRNAs).

    Science.gov (United States)

    Higuchi, Hiroshi

    2012-11-01

    Hypothalamic neuropeptides play important roles in central feeding behavior. Among them, neuropeptide Y (NPY) has the strongest orexigenic action. It is synthesized in NPY-expressing neurons in the arcuate nucleus (ARC), which projects to other nuclei, mainly to the paraventricular nucleus (PVN). PVN, which possesses NPY-Y1, -Y2 and -Y4, -Y5 receptors, is considered as feeding center for central feeding behavior. Herein I review recent results on feeding behavior obtained by gene knockdown technologies. The small interfering RNA (siRNA) plasmid-based vectors, which drive transcription of siRNA by U6 RNA polymerase III promoter to produce knockdown of the NPY and its receptor (Y1, Y2, Y4 and Y5) genes, were stereotaxically injected into mouse ARC and PVN. Feeding behaviors were measured for 6days after siRNA vector injection. NPY and its receptor mRNA levels were decreased, which were measured by RT-PCR and in situ hybridization, and simultaneous decrease in their proteins was also detected in separate nuclei by immunohistochemistry. In the NPY system, decrease in NPY, Y1 and Y5 expressions in specialized nuclei diminished central feeding behavior, whereas decrease in Y2 or Y4 expression in both ARC or PVN did not affect feeding behavior. Thus, specialized change in expressions of NPY and its receptors (especially Y1 and Y5) are important for regulation of endogenous feeding behavior in central regulation. Further analysis of NPY receptors may provide better understanding of feeding behavior and of potential therapeutic targets.

  14. Single production of X±5 /3 and Y∓4 /3 vectorlike quarks at the LHC

    Science.gov (United States)

    Chen, Chuan-Hung; Nomura, Takaaki

    2016-08-01

    Two triplet vectorlike quarks (VLQs) with hypercharges of Y =2 /3 , -1 /3 and one singlet scalar boson are embedded in the standard model to resolve the 750 GeV diphoton excess. The constraints on the tree-level Higgs- and Z -mediated flavor-changing neutral currents are discussed in detail. Besides the resolution of excess, it is found that the signal strength of diphoton Higgs decay can have a 10% deviation from the SM prediction and that the upper limits of the branching ratios for rare top-quark decays are BR (t →c (h ,Z ))<(6.8 ,0.48 )×10-5 . We find that the production cross section of a single VLQ by electroweak processes is larger than that of a VLQ pair by QCD processes. To explore the signals of the heavy VLQs at the LHC, we thoroughly analyze the production of single X±5 /3 and Y∓4 /3 via qiqj' annihilations in p p collisions at √{s }=13 TeV . It is found that the electroweak production cross sections for d X5 /3, u Y-4 /3, and d Y4 /3 channels with mX=mY=1 TeV can be 84.3, 72.3, and 157.8 fb, respectively, and the dominant decay modes are X5 /3→(c ,t )W+ and Y-4 /3→(s ,b )W-. With adopting kinematic cuts, the significance for the p p →d W+t channel can be over 5 σ .

  15. A new method to investigate how mechanical loading of osteocytes controls osteoblasts.

    Science.gov (United States)

    Vazquez, Marisol; Evans, Bronwen A J; Riccardi, Daniela; Evans, Sam L; Ralphs, Jim R; Dillingham, Christopher Mark; Mason, Deborah J

    2014-01-01

    Mechanical loading, a potent stimulator of bone formation, is governed by osteocyte regulation of osteoblasts. We developed a three-dimensional (3D) in vitro co-culture system to investigate the effect of loading on osteocyte-osteoblast interactions. MLO-Y4 cells were embedded in type I collagen gels and MC3T3-E1(14) or MG63 cells layered on top. Ethidium homodimer staining of 3D co-cultures showed 100% osteoblasts and 86% osteocytes were viable after 7 days. Microscopy revealed osteoblasts and osteocytes maintain their respective ovoid/pyriform and dendritic morphologies in 3D co-cultures. Reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR) of messenger ribonucleic acid (mRNA) extracted separately from osteoblasts and osteocytes, showed that podoplanin (E11), osteocalcin, and runt-related transcription factor 2 mRNAs were expressed in both cell types. Type I collagen (Col1a1) mRNA expression was higher in osteoblasts (P MLO-Y4/MG63 co-cultures after 5 days treatment. A 16-well silicone plate, loaded (5 min, 10 Hz, 2.5 N) to induce 4000-4500 με cyclic compression within gels increased prostaglandin E2 (PGE2) release 0.5 h post-load in MLO-Y4 cells pre-cultured in 3D collagen gels for 48, 72 h, or 7 days. Mechanical loading of 3D co-cultures increased type I pro-collagen release 1 and 5 days later. These methods reveal a new osteocyte-osteoblast co-culture model that may be useful for investigating mechanically induced osteocyte control of osteoblast bone formation. PMID:25538684

  16. MT1-MMP modulates the mechanosensitivity of osteocytes.

    Science.gov (United States)

    Kulkarni, Rishikesh N; Bakker, Astrid D; Gruber, Elisabeth V; Chae, Thomas D; Veldkamp, Joris B B; Klein-Nulend, Jenneke; Everts, Vincent

    2012-01-13

    Membrane-type matrix metalloproteinase-1 (MT1-MMP) is expressed by mechanosensitive osteocytes and affects bone mass. The extracellular domain of MT1-MMP is connected to extracellular matrix, while its intracellular domain is a strong modulator of cell signaling. In theory MT1-MMP could thus transduce mechanical stimuli into a chemical response. We hypothesized that MT1-MMP plays a role in the osteocyte response to mechanical stimuli. MT1-MMP-positive and knockdown (siRNA) MLO-Y4 osteocytes were mechanically stimulated with a pulsating fluid flow (PFF). Focal adhesions were visualized by paxillin immunostaining. Osteocyte number, number of empty lacunae, and osteocyte morphology were measured in long bones of MT1-MMP(+/+) and MT1-MMP(-/-) mice. PFF decreased MT1-MMP mRNA and protein expression in MLO-Y4 osteocytes, suggesting that mechanical loading may affect pericellular matrix remodeling by osteocytes. MT1-MMP knockdown enhanced NO production and c-jun and c-fos mRNA expression in response to PFF, concomitantly with an increased number and size of focal adhesions, indicating that MT1-MMP knockdown osteocytes have an increased sensitivity to mechanical loading. Osteocytes in MT1-MMP(-/-) bone were more elongated and followed the principle loading direction, suggesting that they might sense mechanical loading. This was supported by a lower number of empty lacunae in MT1-MMP(-/-) bone, as osteocytes lacking mechanical stimuli tend to undergo apoptosis. In conclusion, mechanical stimulation decreased MT1-MMP expression by MLO-Y4 osteocytes, and MT1-MMP knockdown increased the osteocyte response to mechanical stimulation, demonstrating a novel and unexpected role for MT1-MMP in mechanosensing. PMID:22202174

  17. Acute-phase protein serum amyloid A3 is a novel paracrine coupling factor that controls bone homeostasis.

    Science.gov (United States)

    Thaler, Roman; Sturmlechner, Ines; Spitzer, Silvia; Riester, Scott M; Rumpler, Monika; Zwerina, Jochen; Klaushofer, Klaus; van Wijnen, Andre J; Varga, Franz

    2015-04-01

    Serum amyloid A (A-SAA/Saa3) was shown before to affect osteoblastic metabolism. Here, using RT-quantitative PCR and/or immunoblotting, we show that expression of mouse Saa3 and human SAA1 and SAA2 positively correlates with increased cellular maturation toward the osteocyte phenotype. Expression is not detected in C3H10T1/2 embryonic fibroblasts but is successively higher in preosteoblastic MC3T3-E1 cells, late osteoblastic MLO-A5 cells, and MLO-Y4 osteocytes, consistent with findings using primary bone cells from newborn mouse calvaria. Recombinant Saa3 protein functionally inhibits osteoblast differentiation as reflected by reductions in the expression of osteoblast markers and decreased mineralization in newborn mouse calvaria. Yet, Saa3 protein enhances osteoclastogenesis in mouse macrophages/monocytes based on the number of multinucleated and tartrate-resistant alkaline phosphatase-positive cells and Calcr mRNA expression. Depletion of Saa3 in MLO osteocytes results in the loss of the mature osteocyte phenotype. Recombinant osteocalcin, which is reciprocally regulated with Saa3 at the osteoblast/osteocyte transition, attenuates Saa3 expression in MLO-Y4 osteocytes. Mechanistically, Saa3 produced by MLO-Y4 osteocytes is integrated into the extracellular matrix of MC3T3-E1 osteoblasts, where it associates with the P2 purinergic receptor P2rx7 to stimulate Mmp13 expression via the P2rx7/MAPK/ERK/activator protein 1 axis. Our data suggest that Saa3 may function as an important coupling factor in bone development and homeostasis. PMID:25491310

  18. Estudio epidemiológico de los trastornos de la conducta alimentaria en población escolarizada en el estado de Nayarit, México

    OpenAIRE

    Negrete Castellano, María América

    2015-01-01

    Objetivo: Investigar los problemas de la conducta alimentaria mediante un estudio epidemiológico de dos fases en la población adolescente escolarizada del estado de Nayarit, México. Método: Se consideraron 13 de los 20 municipios que conforman el estado, 9 de ellos urbanos y 4 rurales. La muestra total constó de 2449 participantes, 1142 chicos (46,6%) y 1307 chicas (53,4%) de 11 a 20 años (M = 15,5; DE = 1,8), todos ellos estudiantes de educación secundaria y de bachillerato de 27 centros ...

  19. Consumo de tabaco de los adolescentes en Barcelona y tendencias a lo largo de 20 años

    OpenAIRE

    Carles Ariza; Xavier García-Continente; Joan Ramon Villalbí; Francesca Sánchez-Martínez; Anna Pérez; Manel Nebot

    2014-01-01

    Objetivos: El tabaquismo es causa de muerte prematura y prevenible, que se inicia en la adolescencia. Se describe el consumo de tabaco en escolares durante 2008 y la tendencia en los últimos 20 años en Barcelona. Material y métodos: Estudio de análisis de la tendencia del consumo. Se comparan datos de ocho encuestas realizadas entre 1987 y 2008 en 2° y 4° cursos de Educación Secundaria Obligatoria (ESO) y 2° de Bachillerato o Ciclos Formativos de Grado Medio. Se utilizó el cuestionario FRE...

  20. Detección del fenómeno de Bell previo al reflejo pupilar a la luz

    OpenAIRE

    Espinosa Tomás, Julián; Pérez Rodríguez, Jorge; Mas Candela, David

    2015-01-01

    El desarrollo de un video-oculógrafo binocular con resolución 15 micras y 4 ms orientado a la caracterización de los reflejos pupilares ha revelado la existencia de un movimiento de elevación del globo ocular, conocido como fenómeno de Bell, previo al reflejo pupilar a la luz. Además, su uso para el análisis del reflejo pupilar a la luz ha confirmado que las respuestas pupilares directa y consensual no presentan ninguna diferencia temporal apreciable Contribución como póster al Simposio d...

  1. Initial Scraper Measurements in SPEAR 3

    CERN Document Server

    Corbett, Jeff; Terebilo, Andrei

    2005-01-01

    Horizontal and vertical scraper assemblies have been installed in a standard straight section of SPEAR 3 (beta-x=10.2 m, beta-y=4.8 m, epsx=18 nm-rad, 0.1% coupling). Each scraper has stepper motor accuracy and transductor resolution on the order of 1 micron and a MATLAB/EPICS interface to simplify data acquisition. In this paper we present initial scraper measurements and analysis of the results. Of particular importance are comparison of measured dynamic aperture and momentum acceptance with model and measurement of vertical acceptance for future beam lines.

  2. 《函数及其图象》综合测试题

    Institute of Scientific and Technical Information of China (English)

    大勇

    2007-01-01

    @@ 1.函数y=1-x-2中,自变量x的取值范围是( ). A.x<2 B.x=2 C.x>2 D.x≠2 2.函数值y随着x的增大而减小的是( ). A.y=2+x B.y=5x C.y=1-x D.y=-2+3x 3.函数的图象不经过第二象限的是( ). A.y=-2x+3 B.y=2x+1 C.y=5x-1 D.y=-4x-4

  3. Dalitz Analysis of Three-body Charmless B0=>K0pi+pi- Decay

    CERN Document Server

    Garmash, A; Adachi, I; Aihara, H; Anipko, D; Arinstein, K; Aulchenko, V; Aushev, T; Bahinipati, S; Bakich, A M; Balagura, V; Barberio, E; Barbero, M; Bay, A; Bedny, I; Belous, K S; Bitenc, U; Bizjak, I; Blyth, S; Bondar, A; Bozek, A; Bracko, M; Browder, T E; Chang, M C; Chao, Y; Chen, A; Chen, K F; Chen, W T; Cheon, B G; Chistov, R; Choi, Y; Choi, Y K; Cole, S; Dalseno, J; Dash, M; Dragic, J; Drutskoy, A; Eidelman, S; Epifanov, D A; Gabyshev, N; Gershon, T; Go, A; Gokhroo, G; Golob, B; Ha, H; Haba, J; Hayasaka, K; Hayashii, H; Hazumi, M; Heffernan, D; Hokuue, T; Hoshi, Y; Hou, S; Hou, W S; Hsiung, Y B; Iijima, T; Ikado, K; Imoto, A; Inami, K; Ishikawa, A; Ishino, H; Itoh, R; Iwasaki, M; Iwasaki, Y; Kang, J H; Kapusta, P; Kawai, H; Kawasaki, T; Kichimi, H; Kim, H J; Kim, Y J; Kinoshita, K; Krizan, P; Krokovnyi, P P; Kulasiri, R; Kumar, R; Kuo, C C; Kuzmin, A; Kwon, Y J; Lee, S E; Lesiak, T; Li, J; Lin, S W; Liu, Y; Majumder, G; Mandl, F; Matsumoto, T; McOnie, S; Mitaroff, W A; Miyabayashi, K; Miyake, H; Miyata, H; Miyazaki, Y; Mizuk, R; Mohapatra, D; Moloney, G R; Nagasaka, Y; Nakano, E; Nakao, M; Natkaniec, Z; Nishida, S; Nitoh, O; Noguchi, S; Ohshima, T; Okuno, S; Olsen, S L; Onuki, Y; Pakhlov, P; Pakhlova, G; Park, H; Peak, L S; Pestotnik, R; Piilonen, L E; Poluektov, A; Sahoo, H; Sakai, Y; Satoyama, N; Schietinger, T; Schneider, O; Schümann, J; Schwanda, C; Schwartz, A J; Senyo, K; Shapkin, M; Shibuya, H; Shwartz, B; Sidorov, V; Sokolov, A; Somov, A; Stanic, S; Staric, M; Stöck, H; Sumisawa, K; Sumiyoshi, T; Suzuki, S Y; Takasaki, F; Tamai, K; Tanaka, M; Taylor, G N; Teramoto, Y; Tian, X C; Trabelsi, K; Tsukamoto, T; Uehara, S; Uglov, T; Ueno, K; Unno, Y; Uno, S; Urquijo, P; Ushiroda, Y; Usov, Yu; Varner, G; Varvell, K E; Villa, S; Wang, C H; Wang, M Z; Watanabe, Y; Won, E; Wu, C H; Xie, Q L; Yabsley, B D; Yamaguchi, A; Yamashita, Y; Yamauchi, M; Zhang, C C; Zhang, L M; Zhang, Z P; Zhilich, V; Zupanc, A

    2007-01-01

    We report results of a Dalitz plot analysis of the three-body charmless B0=>K0pi+pi- decay. The analysis is performed with a data sample that contains 388 million BBbar pairs collected near the Y(4S) resonance with the Belle detector at the KEKB asymmetric energy e+e- collider. Measurements of branching fractions for the quasi-two-body decays B0=>rho(770)0K0, B0=>f0(980)K0, B0=>K*(892)+pi-, B0=>K*(1430)+pi-, and upper limits on several other quasi-two-body decay modes are reported.

  4. Caracterización de un robot para aplicaciones de mecanizado con requerimientos de tolerancias

    OpenAIRE

    Ferreras Higuero, Eugenio

    2014-01-01

    El presente Trabajo fin Fin de Máster, versa sobre una caracterización preliminar del comportamiento de un robot de tipo industrial, configurado por 4 eslabones y 4 grados de libertad, y sometido a fuerzas de mecanizado en su extremo. El entorno de trabajo planteado es el de plantas de fabricación de piezas de aleaciones de aluminio para automoción. Este tipo de componentes parte de un primer proceso de fundición que saca la pieza en bruto. Para series medias y altas, en función de las pr...

  5. Frecuencia espontánea en inducida de micronúcleos transplacentarios en ratas Sprague Dawley Spontaneous and induced frequency of transplacental micronucleus in Sprague Dawley rats

    OpenAIRE

    D.F. Arencibia; Rosario, L.A.; Y.E Suárez; Delgado, L.

    2011-01-01

    El presente trabajo tuvo como objetivo determinar la frecuencia espontánea e inducida de micronúcleos transplacentarios en ratas Sprague Dawley, demostrando el efecto clastogénico o aneugénico en el feto a través de la exposición materna y su vinculación con el efecto genotóxico y reproductivo. Se formaron 4 grupos experimentales, 1) control negativo (simulacro), 2) control solvente NaCl (0,9%), 3) ciclofosfamida 50 mg/kg, y 4) bleomicina 30 mg/kg. Todos los grupos se administraron por vía in...

  6. Plan de negocios fabricación y distribución de cosméticos para hombres

    OpenAIRE

    Montaña León, Diana Ivonne; Mesa Silva, Miguel Ángel

    2012-01-01

    En el presente documento se presentan los estudios de mercado, técnico, organizacional y financiero para finalmente a través de los mismos determinar la viabilidad del modelo de negocio planteado. Plan de negocios para la fabricación y distribución de productos cosméticos para hombres. Dicho plan esta enfocado a los estratos 3 y 4. Por otro lado en el documento se puede apreciar todas las consideraciones acerca del diseño organizacional y financiero además del estudio de mercado.

  7. Calculation of particle and quark masses solely using the fine structure constant alpha, the nucleon/electron mass ratio beta and the electron mass

    International Nuclear Information System (INIS)

    A surprisingly simple relationship for particle and quark masses is given as m = x*y*me. Thereby y = 1 and x = 1/α, β and β/α for a hypothetic mass m0, the nucleon and the Higgs boson. With y = 4/3 instead y = 1 one obtains the masses of the strange-, charm-, and top quark, with x = β/α and y = 2/π the Z boson and with the π-2 fold thereof the W boson. The aforementioned m0 is the building block for calculating, as integer multiples, all other meson- and baryon masses with better than 2 % accuracy.

  8. Evaluación del porcentaje de material núcleo en conductos radiculares obturados con puntas de gutapercha y de Real Seal de distintas conicidades

    OpenAIRE

    Brito Plasencia, M. J.; Canalda Sahli, Carlos

    2007-01-01

    Objetivo. El objetivo del presente estudio fue evaluar el porcentaje de aterial núcleo en conductos radiculares de dientes monorradiculares obturados mediante compactación lateral y puntas de gutapercha o de Real Seal de conicidades 2% y 4% junto con un sellador. Material y métodos. Se emplearon 40 conductos de dientes maxilares anteriores. Se prepararon los conductos mediante rotación horaria continua hasta un calibre 30/.04. Se obturaron mediante compactación lateral en frío. Se formaron al...

  9. Protocolo de valoración, seguimiento y actuación nutricional en un centro residencial para personas mayores A protocol of assessment, follow-up and nutritional action at a nursing home

    OpenAIRE

    C. Abajo del Álamo; S. García Rodicio; B. Calabozo Freile; L. Ausín Pérez; J. Casado Pérez; M. A. Catalá Pindado

    2008-01-01

    Objetivo: 1) Evaluar el estado nutricional de los ancianos válidos institucionalizados en un Centro Residencial de la Tercera Edad; 2) Proponer las intervenciones nutricionales necesarias, y 3) Establecer un protocolo consensuado de valoración y seguimiento nutricional en el Centro. Método: Estudio transversal sobre la totalidad de los residentes válidos, realizando: 1) Test Mini Nutricional Assessment; 2) Valoración antropométrica; 3) Valoración bioquímica, y 4) cuestionario adicional (infor...

  10. Respuesta fisiológica de cilantro (coriandrum sativum l.) a la disponibilidad de agua en el suelo

    OpenAIRE

    Mejia de Tafur, Maria Sara; Menjivar Flores, Juan Carlos; Marin Pimentel, Gilberto Eduardo

    2014-01-01

    Se realizó un experimento con el fin de determinar los requerimientos hídricos del cilantro variedad Unapal Precoso y relacionarlos con el rendimiento de follaje fresco y materia seca.  Se sembraron plantas en macetas con 6 kg de suelo y se aplicaron láminas de riego de 140; 160; 200 (testigo propuesta por Vallejo et al, 2004); 240 y 280 mm. Se utilizó un diseño completamente al azar con 5 tratamientos y 4 repeticiones, con 36 plantas por unidad experimental. Se realizaron mediciones ...

  11. Calidad de la canal y carne de corderos complementados con aceites y rastrojo de maíz.

    OpenAIRE

    Hernández Cruz, Laura

    2011-01-01

    El estudio se realizó con 48 corderos de la cruza Pelibuey x Katahdin de aproximadamente 4 meses de edad con un peso promedio de 29 ±1.2 kg los cuales se distribuyeron en un diseño completamente al azar, en arreglo factorial 2x4; los tratamientos fueron: 1) Sin aceite (C), 2) 3% de aceite con ácido linoleico conjugado (CLA), 3) 3% de aceite con ácido linoleico (LA) y 4) 3% de aceite de linaza (LZ); y dos porcentajes de rastrojo de maíz por kg de MS-1 (16 y 26). Los resultados en GDP y CMS/GDP...

  12. Measurement of CP-Violating Asymmetries in B0-->(rhopi)0 Using a Time-Dependent Dalitz Plot Analysis

    CERN Document Server

    Aubert, B; Boutigny, D; Karyotakis, Yu; Lees, J P; Poireau, V; Prudent, X; Tisserand, V; Zghiche, A; Graugès-Pous, E; Garra Tico, J; Palano, A; López, L; Eigen, G; Ofte, I; Stugu, B; Sun, L; Abrams, G S; Battaglia, M; Brown, D N; Button-Shafer, J; Cahn, R N; Groysman, Y; Jacobsen, R G; Kadyk, J A; Kerth, L T; Kolomensky, Yu G; Kukartsev, G; Lopes-Pegna, D; Lynch, G; Mir, L M; Orimoto, T J; Pripstein, M; Roe, N A; Ronan, M T; Tackmann, K; Wenzel, W A; Del Amo-Sánchez, P; Hawkes, C M; Watson, A T; Held, T; Koch, H; Lewandowski, B; Pelizaeus, M; Schröder, T; Steinke, M; Boyd, J T; Burke, J P; Cottingham, W N; Walker, D; Asgeirsson, D J; Çuhadar-Dönszelmann, T; Fulsom, B G; Hearty, C; Knecht, N S; Mattison, T S; McKenna, J A; Khan, A; Saleem, M; Teodorescu, L; Blinov, V E; Bukin, A D; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, K Yu; Bondioli, M; Bruinsma, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M A; Martin, E C; Stoker, D P; Buchanan, C; Abachi, S; Foulkes, S D; Gary, J W; Liu, F; Long, O; Shen, B C; Zhang, L; Paar, H P; Rahatlou, S; Sharma, V; Berryhill, J W; Campagnari, C; Cunha, A; Dahmes, B; Hong, T M; Kovalskyi, D; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Schalk, T; Schumm, B A; Seiden, A; Williams, D C; Wilson, M G; Winstrom, L O; Chen, E; Cheng, C H; Dvoretskii, A; Fang, F; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Mancinelli, G; Meadows, B T; Mishra, K; Sokoloff, M D; Blanc, F; Bloom, P C; Chen, S; Ford, W T; Hirschauer, J F; Kreisel, A; Nagel, M; Nauenberg, U; Olivas, A; Smith, J G; Ulmer, K A; Wagner, S R; Zhang, J; Chen, A; Eckhart, E A; Soffer, A; Toki, W H; Wilson, R J; Winklmeier, F; Zeng, Q; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Merkel, J; Petzold, A; Spaan, B; Wacker, K; Brandt, T; Klose, V; Lacker, H M; Mader, W F; Nogowski, R; Schubert, J; Schubert, K R; Schwierz, R; Sundermann, J E; Volk, A; Bernard, D; Bonneaud, G R; Latour, E; Thiebaux, C; Verderi, M; Clark, P J; Gradl, W; Muheim, F; Playfer, S; Robertson, A I; Xie, Y; Andreotti, M; Bettoni, D; Bozzi, C; Calabrese, R; Cecchi, A; Cibinetto, G; Franchini, P; Luppi, E; Negrini, M; Petrella, A; Piemontese, L; Prencipe, E; Santoro, V; Anulli, F; Baldini-Ferroli, R; Calcaterra, A; De Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzo, A; Contri, R; Lo Vetere, M; Macri, M M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Chaisanguanthum, K S; Morii, M; Wu, J; Dubitzky, R S; Marks, J; Schenk, S; Uwer, U; Bard, D J; Dauncey, P D; Flack, R L; Nash, J A; Nikolich, M B; Panduro-Vazquez, W; Behera, P K; Chai, X; Charles, M J; Mallik, U; Meyer, N T; Ziegler, V; Cochran, J; Crawley, H B; Dong, L; Eyges, V; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Lae, C K; Gritsan, A V; Denig, A G; Fritsch, M; Schott, G; Arnaud, N; Bequilleux, J; Davier, M; Grosdidier, G; Höcker, A; Lepeltier, V; Le Diberder, F R; Lutz, A M; Pruvot, S; Rodier, S; Roudeau, P; Schune, M H; Serrano, J; Sordini, V; Stocchi, A; Wang, W F; Wormser, G; Wright, D M; Lange, D J; Chavez, C A; Forster, I J; Fry, J R; Gabathuler, E; Gamet, R; Hutchcroft, D E; Payne, D J; Schofield, K C; Touramanis, C; Bevan, A J; George, K A; Di Lodovico, F; Menges, W; Sacco, R; Cowan, G; Flächer, H U; Hopkins, D A; Jackson, P S; McMahon, T R; Salvatore, F; Wren, A C; Davis,C L; Allison, J; Barlow, N R; Barlow, R J; Chia, Y M; Edgar, C L; Lafferty, G D; West, T J; Yi, J I; Anderson, J; Chen, C; Jawahery, A; Roberts, D A; Simi, G; Tuggle, J M; Blaylock, G; Dallapiccola, C; Hertzbach, S S; Li, X; Moore, T B; Salvati, E; Saremi, S; Cowan, R; Fisher, P H; Sciolla, G; Sekula, S J; Spitznagel, M; Taylor, F; Yamamoto, R K; Kim, H; Mclachlin, S E; Patel, P M; Robertson, S H; Lazzaro, A; Lombardo, V; Palombo, F; Bauer, J M; Cremaldi, L; Eschenburg, V; Godang, R; Kroeger, R; Sanders, D A; Summers, D J; Zhao, H W; Brunet, S; Côté, D; Simard, M; Taras, P; Viaud, F B; Nicholson, H; De Nardo, Gallieno; Fabozzi, F; Lista, L; Monorchio, D; Sciacca, C; Baak, M A; Raven, G; Snoek, H L; LoSecco, J M; Jessop, C P; Benelli, G; Corwin, L A; Gan, K K; Honscheid, K; Hufnagel, D; Kagan, H; Kass, R; Morris, J P; Rahimi, A M; Regensburger, J J; Ter-Antonian, R; Wong, Q K; Blount, N L; Brau, J E; Frey, R; Igonkina, O; Kolb, J A; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Gagliardi, N; Gaz, A; Margoni, M; Morandin, M; Pompili, A; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; Ben-Haim, E; Briand, H; Chauveau, J; David, P; Del Buono, L; De La Vaissière, C; Hamon, O; Hartfiel, B L; Leruste, P; Malcles, J; Ocariz, J; Pérez, A; Gladney, L; Biasini, M; Covarelli, R; Manoni, E; Angelini, C; Batignani, G; Bettarini, S; Calderini, G

    2007-01-01

    We report a measurement of CP-violating asymmetries in B0-->(rhopi)0-->pi+pi-pi0 decays using a time-dependent Dalitz plot analysis. The results are obtained from a data sample of 375 million Y(4S) --> BBbar decays, collected by the BaBar detector at the PEP-II asymmetric-energy B Factory at SLAC. We measure 26 coefficients of the bilinear form-factor terms occurring in the time-dependent decay rate of the B0 meson. We derive the physically relevant quantities from these coefficients. In particular, we measure a constraint on the angle alpha of the Unitarity Triangle.

  13. Search for the rare decay B to pi l+ l-

    CERN Document Server

    Aubert, B; Boutigny, D; Karyotakis, Yu; Lees, J P; Poireau, V; Prudent, X; Tisserand, V; Zghiche, A; Garra Tico, J; Graugès-Pous, E; López, L; Palano, A; Eigen, G; Ofte, I; Stugu, B; Sun, L; Abrams, G S; Battaglia, M; Brown, D N; Button-Shafer, J; Cahn, R N; Groysman, Y; Jacobsen, R G; Kadyk, J A; Kerth, L T; Kolomensky, Yu G; Kukartsev, G; Lopes-Pegna, D; Lynch, G; Mir, L M; Orimoto, T J; Pripstein, M; Roe, N A; Ronan, M T; Tackmann, K; Wenzel, W A; Del Amo-Sánchez, P; Hawkes, C M; Watson, A T; Held, T; Koch, H; Lewandowski, B; Pelizaeus, M; Schröder, T; Steinke, M; Boyd, J T; Burke, J P; Cottingham, W N; Walker, D; Asgeirsson, D J; Çuhadar-Dönszelmann, T; Fulsom, B G; Hearty, C; Knecht, N S; Mattison, T S; McKenna, J A; Khan, A; Saleem, M; Teodorescu, L; Blinov, V E; Bukin, A D; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, K Yu; Bondioli, M; Bruinsma, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M A; Martin, E C; Stoker, D P; Abachi, S; Buchanan, C; Foulkes, S D; Gary, J W; Liu, F; Long, O; Shen, B C; Zhang, L; Paar, H P; Rahatlou, S; Sharma, V; Berryhill, J W; Campagnari, C; Cunha, A; Dahmes, B; Hong, T M; Kovalskyi, D; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Schalk, T; Schumm, B A; Seiden, A; Williams, D C; Wilson, M G; Winstrom, L O; Chen, E; Cheng, C H; Dvoretskii, A; Fang, F; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Mancinelli, G; Meadows, B T; Mishra, K; Sokoloff, M D; Blanc, F; Bloom, P C; Chen, S; Ford, W T; Hirschauer, J F; Kreisel, A; Nagel, M; Nauenberg, U; Olivas, A; Smith, J G; Ulmer, K A; Wagner, S R; Zhang, J; Chen, A; Eckhart, E A; Soffer, A; Toki, W H; Wilson, R J; Winklmeier, F; Zeng, Q; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Merkel, J; Petzold, A; Spaan, B; Wacker, K; Brandt, T; Klose, V; Lacker, H M; Mader, W F; Nogowski, R; Schubert, J; Schubert, K R; Schwierz, R; Sundermann, J E; Volk, A; Bernard, D; Bonneaud, G R; Latour, E; Thiebaux, C; Verderi, M; Clark, P J; Gradl, W; Muheim, F; Playfer, S; Robertson, A I; Xie, Y; Andreotti, M; Bettoni, D; Bozzi, C; Calabrese, R; Cecchi, A; Cibinetto, G; Franchini, P; Luppi, E; Negrini, M; Petrella, A; Piemontese, L; Prencipe, E; Santoro, V; Anulli, F; Baldini-Ferroli, R; Calcaterra, A; De Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzo, A; Contri, R; Lo Vetere, M; Macri, M M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Chaisanguanthum, K S; Morii, M; Wu, J; Dubitzky, R S; Marks, J; Schenk, S; Uwer, U; Bard, D J; Dauncey, P D; Flack, R L; Nash, J A; Nikolich, M B; Panduro-Vazquez, W; Behera, P K; Chai, X; Charles, M J; Mallik, U; Meyer, N T; Ziegler, V; Cochran, J; Crawley, H B; Dong, L; Eyges, V; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Gritsan, A V; Lae, C K; Denig, A G; Fritsch, M; Schott, G; Arnaud, N; Bequilleux, J; Davier, M; Grosdidier, G; Höcker, A; Lepeltier, V; Le Diberder, F R; Lutz, A M; Pruvot, S; Rodier, S; Roudeau, P; Schune, M H; Serrano, J; Sordini, V; Stocchi, A; Wang, W F; Wormser, G; Lange, D J; Wright, D M; Chavez, C A; Forster, I J; Fry, J R; Gabathuler, E; Gamet, R; Hutchcroft, D E; Payne, D J; Schofield, K C; Touramanis, C; Bevan, A J; George, K A; Di Lodovico, F; Menges, W; Sacco, R; Cowan, G; Flächer, H U; Hopkins, D A; Jackson, P S; McMahon, T R; Salvatore, F; Wren, A C; Davis, C L; Allison, J; Barlow, N R; Barlow, R J; Chia, Y M; Edgar, C L; Lafferty, G D; West, T J; Yi, J I; Anderson, J; Chen, C; Jawahery, A; Roberts, D A; Simi, G; Tuggle, J M; Blaylock, G; Dallapiccola, C; Hertzbach, S S; Li, X; Moore, T B; Salvati, E; Saremi, S; Cowan, R; Fisher, P H; Sciolla, G; Sekula, S J; Spitznagel, M; Taylor, F; Yamamoto, R K; Kim, H; Mclachlin, S E; Patel, P M; Robertson, S H; Lazzaro, A; Lombardo, V; Palombo, F; Bauer, J M; Cremaldi, L; Eschenburg, V; Godang, R; Kroeger, R; Sanders, D A; Summers, D J; Zhao, H W; Brunet, S; Côté, D; Simard, M; Taras, P; Viaud, F B; Nicholson, H; De Nardo, Gallieno; Fabozzi, F; Lista, L; Monorchio, D; Sciacca, C; Baak, M A; Raven, G; Snoek, H L; Jessop, C P; LoSecco, J M; Benelli, G; Corwin, L A; Gan, K K; Honscheid, K; Hufnagel, D; Kagan, H; Kass, R; Morris, J P; Rahimi, A M; Regensburger, J J; Ter-Antonian, R; Wong, Q K; Blount, N L; Brau, J E; Frey, R; Igonkina, O; Kolb, J A; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Gagliardi, N; Gaz, A; Margoni, M; Morandin, M; Pompili, A; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; Ben-Haim, E; Briand, H; Chauveau, J; David, P; Del Buono, L; La Vaissière, C de; Hamon, O; Hartfiel, B L; Leruste, P; Malcles, J; Ocariz, J; Pérez, A; Gladney, L; Biasini, M; Covarelli, R; Manoni, E; Angelini, C; Batignani, G; Bettarini, S; Calderini, G

    2007-01-01

    We have performed a search for the flavor-changing neutral-current decays B --> pi l+l-, where l+l- is either e+e- or mu+mu-, using a sample of 230 --> million Y(4S)-->BBbar decays collected with the BABAR detector. We observe no evidence of a signal and measure the upper limit on the isospin-averaged branching fraction to be BF(B --> pi l+ l-) pi e mu and measure an upper limit on the isospin-averaged branching fraction of BF(B --> pi e mu) < 9.2 x 10^{-8} at 90% confidence level.

  14. Search for B+ meson decay to a1+ K*0

    CERN Document Server

    Aubert, B; Karyotakis, Yu; Lees, J P; Poireau, V; Prencipe, E; Prudent, X; Tisserand, V; Garra Tico, J; Graugès-Pous, E; López, L; Palano, A; Pappagallo, M; Eigen, G; Stugu, B; Sun, L; Abrams, G S; Battaglia, M; Brown, D N; Cahn, R N; Jacobsen, R G; Kerth, L T; Kolomensky, Yu G; Lynch, G; Osipenkov, I L; Ronan, M T; Tackmann, K; Tanabé, T; Hawkes, C M; Soni, N; Watson, A T; Koch, H; Schröder, T; Walker, D; Asgeirsson, D J; Fulsom, B G; Hearty, C; Mattison, T S; McKenna, J A; Barrett, M; Khan, A; Blinov, V E; Bukin, A D; Buzykaev, A R; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, K Yu; Bondioli, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M; Martin, E C; Stoker, D P; Abachi, S; Buchanan, C; Gary, J W; Liu, F; Long, O; Shen, B C; Vitug, G M; Yasin, Z; Zhang, L; Gary, J W; Liu, F; Long, O; Shen, B C; Vitug, G M; Yasin, Z; Zhang, L; Sharma, V; Campagnari, C; Hong, T M; Kovalskyi, D; Mazur, M A; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Martinez, A J; Schalk, T; Schumm, B A; Seiden, A; Wilson, M G; Winstrom, L O; Cheng, C H; Doll, D A; Echenard, B; Fang, F; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Andreassen, R; Mancinelli, G; Meadows, B T; Mishra, K; Sokoloff, M D; Bloom, P C; Ford, W T; Gaz, A; Hirschauer, J F; Nagel, M; Nauenberg, U; Smith, J G; Ulmer, K A; Wagner, S R; Ayad, R; Soffer, A; Toki, W H; Wilson, R J; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Karbach, M; Merkel, J; Petzold, A; Spaan, B; Wacker, K; Kobel, M J; Mader, W F; Nogowski, R; Schubert, K R; Schwierz, R; Volk, A; Bernard, D; Bonneaud, G R; Latour, E; Verderi, M; Clark, P J; Playfer, S; Watson, J E; Andreotti, M; Bettoni, D; Bozzi, C; Calabrese, R; Cecchi, A; Cibinetto, G; Franchini, P; Luppi, E; Negrini, M; Petrella, A; Piemontesea, L; Santoro, V; Baldini-Ferroli, R; Calcaterra, A; De Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzo, A; Contri, R; Lo Vetere, M; Macri, M M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Chaisanguanthum, K S Chaisang K S; Moriiuanthum, M; Morii, M; Adametz, A; Marks, J; Schenk, S; Uwer, U; Klose, V; Lacker, H M; Bard, D J; Dauncey, P D; Nash, J A; Tibbetts, M; Behera, P K; Chai, X; Charles, M J; Mallik, U; Cochran, J; Crawley, H B; Dong, L; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Gao, Y Y; Gritsan, A V; Guo, Z J; Lae, C K; Arnaud, N; Bequilleux, J; D'Orazio, A; Davier, M; Firminoda Costa, J; Grosdidier, G; Höcker, A; Lepeltier, V; Le Diberder, F; Lutz, A M; Pruvot, S; Roudeau, P; Schune, M H; Serrano, J; Sordini, V; Stocchi, A; Wormser, G; Lange, D J; Wright, D M; Bingham, I; Burke, J P; Chavez, C A; Fry, J R; Gabathuler, E; Gamet, R; Hutchcroft, D E; Payne, D J; Touramanis, C; Bevan, A J; Clarke, C K; George, K A; Di Lodovico, F; Sacco, R; Sigamani, M; Cowan, G; Cowan, H U Fla G; Flächer, H U; Hopkins, D A; Paramesvaran, S; Salvatore, F; Wrenecher, A C; Hopkins, D A; Paramesvaran, S; Salvatore, F; Wren, A C; Brown, D N; Davis, C L; Fritsch, A G Denig M; Gradl, W; Schott, G; Yi, J I; Anderson, J; Chen, C; Jawahery, A; Roberts, D A; Simi, G; Tuggle, J M; Dallapiccola, C; Li, X; Salvati, E; Saremi, S; Cowan, R; Dujmic, D; Fisher, P H; Sciolla, G; Spitznagel, M; Taylor, F; Yamamoto, R K; Zhao, M; Patel, P M; Robertson, S H; Lazzaro, A; Lombardo, V; Palombo, F; Bauer, J M; Godang, L Cremaldi R; Kroeger, R; Sanders, D A; Summers, D J; Zhao, H W; Simard, M; Taras, P; Viaud, F B; Nicholson, H; De Nardo, Gallieno; Lista, L; Monorchio, D; Onorato, G; Sciacca, C; Raven, G; Snoek, H L; Jessop, C P; Knoepfel, K J; LoSecco, J M; Wang, W F; Benelli, G; Corwin, L A; Honscheid, K; Kagan, H; Kass, R; Morris, J P; Rahimi, A M; Regensburger, J J; Sekula, S J; Wong, Q K; Blount, N L; Brau, J E; Frey, R; Igonkina, O; Kolb, J A; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Castelli, G; Gagliardi, N; Margoni, M; Morandin, M; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; Del Amo-Sánchez, P; Ben-Haim, E; Briand, H; Calderini, G; Chauveau, J; David, P; Del Buono, L; Hamon, O; Leruste, P; Ocariz, J; Pérez, A; Prendki, J; Sitt, S; Gladney, L; Biasini, M; Covarelli, R; Manoni, E; Angelini, C; Batignani, G; Bettarini, S; Carpinelli, M; Cervelli, A; Forti, F; Giorgi, M A; Lusiani, A; Marchiori, G; Morganti, M; Neri, N; Paoloni, E; Rizzo, G; Walsh, J J; Lopes-Pegna, D; Lü, C; Olsen, J; Smith, A J S; Telnov, A V; Anullia, F; Baracchiniab, E; Cavotoa, G; del Reab, D; Di Marcoab, E; Facciniab, R; Ferrarottoa, F; Ferroni, F; Gaspero, M; Jackson, P D; Li Gioi, L; Mazzoni, M A; Morganti, S; Piredda, G; Polci, F; Renga, F; Voena, C; Ebert, M; Hartmann, T; Schröder, H; Waldi, R; Adye, T; Franek, B; Olaiya, E O; Wilson, F F; Emery, S

    2008-01-01

    We present the preliminary result of a search for the decay B+- --> a1+-K*0. The data, collected with the BaBar detector at the Stanford Linear Accelerator Center, represent 465 million BB pairs produced in e+e- annihilation at the Y(4s) energy. The result for the branching fraction is: B(B+ --> a1+ K*0) * B(a1+ --> pi+pi-pi+) = (0.7 +0.5/-0.4 +/-0.7) x 10-6, corresponding to an upper limit at 90% confidence level of 1.6 x 10-6. The first error quoted is statistical, the second systematic.

  15. Improved Measurement of CP Violation in Neutral B Decays to c cbar s

    CERN Document Server

    Aubert, B; Boutigny, D; Karyotakis, Yu; Lees, J P; Poireau, V; Prudent, X; Tisserand, V; Zghiche, A; Garra Tico, J; Graugès-Pous, E; López, L; Palano, A; Eigen, G; Ofte, I; Stugu, B; Sun, L; Abrams, G S; Battaglia, M; Brown, D N; Button-Shafer, J; Cahn, R N; Groysman, Y; Jacobsen, R G; Kadyk, J A; Kerth, L T; Kolomensky, Yu G; Kukartsev, G; Lopes-Pegna, D; Lynch, G; Mir, L M; Orimoto, T J; Pripstein, M; Roe, N A; Ronan, M T; Tackmann, K; Wenzel, W A; Del Amo-Sánchez, P; Hawkes, C M; Watson, A T; Held, T; Koch, H; Lewandowski, B; Pelizaeus, M; Schröder, T; Steinke, M; Cottingham, W N; Walker, D; Asgeirsson, D J; Çuhadar-Dönszelmann, T; Fulsom, B G; Hearty, C; Knecht, N S; Mattison, T S; McKenna, J A; Khan, A; Saleem, M; Teodorescu, L; Blinov, V E; Bukin, A D; Druzhinin, V P; Golubev, V B; Onuchin, A P; Serednyakov, S I; Skovpen, Yu I; Solodov, E P; Todyshev, K Yu; Bondioli, M; Curry, S; Eschrich, I; Kirkby, D; Lankford, A J; Lund, P; Mandelkern, M A; Martin, E C; Stoker, D P; Abachi, S; Buchanan, C; Foulkes, S D; Gary, J W; Liu, F; Long, O; Shen, B C; Zhang, L; Paar, H P; Rahatlou, S; Sharma, V; Berryhill, J W; Campagnari, C; Cunha, A; Dahmes, B; Hong, T M; Kovalskyi, D; Richman, J D; Beck, T W; Eisner, A M; Flacco, C J; Heusch, C A; Kroseberg, J; Lockman, W S; Schalk, T; Schumm, B A; Seiden, A; Williams, D C; Wilson, M G; Winstrom, L O; Chen, E; Cheng, C H; Dvoretskii, A; Fang, F; Hitlin, D G; Narsky, I; Piatenko, T; Porter, F C; Mancinelli, G; Meadows, B T; Mishra, K; Sokoloff, M D; Blanc, F; Bloom, P C; Chen, S; Ford, W T; Hirschauer, J F; Kreisel, A; Nagel, M; Nauenberg, U; Olivas, A; Smith, J G; Ulmer, K A; Wagner, S R; Zhang, J; Gabareen, A M; Soffer, A; Toki, W H; Wilson, R J; Winklmeier, F; Zeng, Q; Altenburg, D D; Feltresi, E; Hauke, A; Jasper, H; Merkel, J; Petzold, A; Spaan, B; Wacker, K; Brandt, T; Klose, V; Lacker, H M; Mader, W F; Nogowski, R; Schubert, J; Schubert, K R; Schwierz, R; Sundermann, J E; Volk, A; Bernard, D; Bonneaud, G R; Latour, E; Lombardo, V; Thiebaux, C; Verderi, M; Clark, P J; Gradl, W; Muheim, F; Playfer, S; Robertson, A I; Xie, Y; Andreotti, M; Bettoni, D; Bozzi, C; Calabrese, R; Cecchi, A; Cibinetto, G; Franchini, P; Luppi, E; Negrini, M; Petrella, A; Piemontese, L; Prencipe, E; Santoro, V; Anulli, F; Baldini-Ferroli, R; Calcaterra, A; De Sangro, R; Finocchiaro, G; Pacetti, S; Patteri, P; Peruzzi, I M; Piccolo, M; Rama, M; Zallo, A; Buzzo, A; Contri, R; Lo Vetere, M; Macri, M M; Monge, M R; Passaggio, S; Patrignani, C; Robutti, E; Santroni, A; Tosi, S; Chaisanguanthum, K S; Morii, M; Wu, J; Dubitzky, R S; Marks, J; Schenk, S; Uwer, U; Bard, D J; Dauncey, P D; Flack, R L; Nash, J A; Nikolich, M B; Panduro-Vazquez, W; Behera, P K; Chai, X; Charles, M J; Mallik, U; Meyer, N T; Ziegler, V; Cochran, J; Crawley, H B; Dong, L; Eyges, V; Meyer, W T; Prell, S; Rosenberg, E I; Rubin, A E; Gritsan, A V; Guo, Z J; Lae, C K; Denig, A G; Fritsch, M; Schott, G; Arnaud, N; Bequilleux, J; Davier, M; Grosdidier, G; Höcker, A; Lepeltier, V; Le Diberder, F R; Lutz, A M; Pruvot, S; Rodier, S; Roudeau, P; Schune, M H; Serrano, J; Sordini, V; Stocchi, A; Wang, W F; Wormser, G; Lange, D J; Wright, D M; Chavez, C A; Forster, I J; Fry, J R; Gabathuler, E; Gamet, R; Hutchcroft, D E; Payne, D J; Schofield, K C; Touramanis, C; Bevan, A J; George, K A; Di Lodovico, F; Menges, W; Sacco, R; Cowan, G; Flächer, H U; Hopkins, D A; Jackson, P S; McMahon, T R; Salvatore, F; Wren, A C; Davis, C L; Allison, J; Barlow, N R; Barlow, R J; Chia, Y M; Edgar, C L; Lafferty, G D; West, T J; Yi, J I; Anderson, J; Chen, C; Jawahery, A; Roberts, D A; Simi, G; Tuggle, J M; Blaylock, G; Dallapiccola, C; Hertzbach, S S; Li, X; Moore, T B; Salvati, E; Saremi, S; Cowan, R; Fisher, P H; Sciolla, G; Sekula, S J; Spitznagel, M; Taylor, F; Yamamoto, R K; Mclachlin, S E; Patel, P M; Robertson, S H; Lazzaro, A; Palombo, F; Bauer, J M; Cremaldi, L; Eschenburg, V; Godang, R; Kroeger, R; Sanders, D A; Summers, D J; Zhao, H W; Brunet, S; Côté, D; Simard, M; Taras, P; Viaud, F B; Nicholson, H; De Nardo, Gallieno; Fabozzi, F; Lista, L; Monorchio, D; Sciacca, C; Baak, M A; Raven, G; Snoek, H L; Jessop, C P; LoSecco, J M; Benelli, G; Corwin, L A; Gan, K K; Honscheid, K; Hufnagel, D; Kagan, H; Kass, R; Morris, J P; Rahimi, A M; Regensburger, J J; Ter-Antonian, R; Wong, Q K; Blount, N L; Brau, J E; Frey, R; Igonkina, O; Kolb, J A; Lu, M; Rahmat, R; Sinev, N B; Strom, D; Strube, J; Torrence, E; Gagliardi, N; Gaz, A; Margoni, M; Morandin, M; Pompili, A; Posocco, M; Rotondo, M; Simonetto, F; Stroili, R; Voci, C; Ben-Haim, E; Briand, H; Chauveau, J; David, P; Del Buono, L; De La Vaissière, C; Hamon, O; Hartfiel, B L; Leruste, P; Malcles, J; Ocariz, J; Pérez, A; Gladney, L; Biasini, M; Covarelli, R; Manoni, E; Angelini, C; Batignani, G; Bettarini, S; Calderini, G; Carpinelli, M; Cenci, R; Cervelli, A

    2007-01-01

    We present updated measurements of time-dependent CP asymmetries in fully-reconstructed neutral B decays to several CP eigenstates containing a charmonium meson. The measurements use a data sample of (383 +/- 4) x 10^6 Y(4S)-> BBbar decays collected with the BABAR detector at the PEP-II B factory. We determine sin2beta = 0.714 +/- 0.032 (stat) +/- 0.018 (syst) and |lambda| = 0.952 +/- 0.022 (stat) +/- 0.017 (syst).

  16. Measurement of CP Asymmetries in B0 --> K0S K0S K0S Decays

    CERN Document Server

    Aubert, B; Abrams, G S; Adye, T; Ahmed, S; Alam, M S; Albert, J; Aleksan, R; Allen, M T; Allison, J; Altenburg, D D; Anderson, J; Andreotti, M; Angelini, C; Anulli, F; Arnaud, N; Asgeirsson, D J; Aston, D; Azzolini, V; Baak, M A; Back, J J; Baldini-Ferroli, R; Band, H R; Banerjee, Sw; Baracchini, E; Bard, D J; Barlow, N R; Barlow, R J; Bartoldus, R; Batignani, G; Battaglia, M; Bauer, J M; Bechtle, P; Beck, T W; Behera, P K; Bellini, F; Ben-Haim, E; Benelli, G; Bequilleux, J; Berger, N; Bernard, D; Berryhill, J W; Bettarini, S; Bettoni, D; Bevan, A J; Bhuyan, B; Bianchi, F; Biasini, M; Biesiada, J; Blanc, F; Blaylock, G; Blinov, V E; Bloom, P C; Blount, N L; Bomben, M; Bondioli, M; Bonneaud, G R; Bosisio, L; Boutigny, D; Boyd, J T; Bozzi, C; Brandt, T; Brau, J E; Briand, H; Brown, D N; Bruinsma, M; Brunet, S; Buchanan, C; Bugg, W; Bukin, A D; Bula, R; Burchat, P R; Burke, J P; Button-Shafer, J; Buzzo, A; Bóna, M; Cahn, R N; Calabrese, R; Calcaterra, A; Calderini, G; Campagnari, C; Carpinelli, M; Cartaro, C; Castelli, G; Cavoto, G; Cecchi, A; Cenci, R; Chai, X; Chaisanguanthum, K S; Charles, M J; Chauveau, J; Chavez, C A; Chen, A; Chen, C; Chen, E; Chen, S; Chen, X; Chen, X R; Cheng, C H; Chia, Y M; Cibinetto, G; Clark, P J; Claus, R; Cochran, J; Coleman, J P; Contri, R; Convery, M R; Corwin, L A; Cossutti, F; Cottingham, W N; Covarelli, R; Cowan, G; Cowan, R; Crawley, H B; Cremaldi, L; Cunha, A; Curry, S; Côté, D; D'Orazio, A; Dahmes, B; Dallapiccola, C; Dasu, S; Dauncey, P D; David, P; Davier, M; Davis, C L; De La Vaissière, C; De Nardo, Gallieno; De Sangro, R; Del Amo-Sánchez, P; Del Buono, L; Del Re, D; Della Ricca, G; Denig, A G; Di Lodovico, F; Di Marco, E; Dingfelder, J C; Dong, L; Dorfan, J; Druzhinin, V P; Dubitzky, R S; Dubois-Felsmann, G P; Dujmic, D; Dunwoodie, W; Dvoretskii, A; Ebert, M; Eckhart, E A; Eckmann, R; Edgar, C L; Edwards, A J; Eigen, G; Eisner, A M; Elmer, P; Emery, S; Ernst, J A; Escalier, M; Eschenburg, V; Eschrich, I; Eyges, V; Fabozzi, F; Faccini, R; Fang, F; Feltresi, E; Ferrarotto, F; Ferroni, F; Field, R C; Finocchiaro, G; Fisher, P H; Flacco, C J; Flack, R L; Flächer, H U; Flood, K T; Ford, W T; Forster, I J; Forti, F; Foulkes, S D; Franchini, P; Franek, B; Frey, R; Fritsch, M; Fry, J R; Fulsom, B G; Gabathuler, E; Gagliardi, N; Gaidot, A; Gallo, F; Gamba, D; Gamet, R; Gan, K K; Ganzhur, S F; Garra Tico, J; Gary, J W; Gaspero, M; Gaz, A; George, K A; Giorgi, M A; Gladney, L; Glanzman, T; Godang, R; Golubev, V B; Gowdy, S J; Gradl, W; Graham, M T; Graugès-Pous, E; Grenier, P; Gritsan, A V; Grosdidier, G; Groysman, Y; Haire, M; Halyo, V; Hamano, K; Hamel de Monchenault, G; Hamon, O; Harrison, P F; Hartfiel, B L; Hast, C; Hauke, A; Hawkes, C M; Hearty, C; Held, T; Hertzbach, S S; Heusch, C A; Hirschauer, J F; Hitlin, D G; Hollar, J J; Hong, T M; Honscheid, K; Hopkins, D A; Hrynóva, T; Hufnagel, D; Hutchcroft, D E; Höcker, A; Igonkina, O; Innes, W R; Izen, J M; Jackson, P D; Jackson, P S; Jacobsen, R G; Jain, V; Jasper, H; Jawahery, A; Jessop, C P; Kadyk, J A; Kagan, H; Karyotakis, Yu; Kass, R; Kelsey, M H; Kerth, L T; Khan, A; Kim, H; Kim, P; Kirkby, D; Klose, V; Knecht, N S; Koch, H; Kolb, J A; Kolomensky, Yu G; Kovalskyi, D; Kowalewski, R V; Kozanecki, W; Kreisel, A; Krishnamurthy, M; Kroeger, R; Kroseberg, J; Kukartsev, G; Kutter, P E; Lacker, H M; Lae, C K; Lafferty, G D; Lanceri, L; Lange, D J; Lankford, A J; Latham, T E; Latour, E; Lau, Y P; Lazzaro, A; Le Diberder, F R; Lees, J P; Legendre, M; Leith, D W G S; Lepeltier, V; Leruste, P; Lewandowski, B; Li Gioi, L; Li, S; Li, X; Lista, L; Liu, F; Liu, H; Lo Vetere, M; LoSecco, J M; Lockman, W S; Lombardo, V; Long, O; Lopes-Pegna, D; Lopez-March, N; Lou, X C; Lu, M; Luitz, S; Lund, P; Luppi, E; Lusiani, A; Lutz, A M; Lynch, G; Lynch, H L; López, L; Lü, C; Lüth, V; MacFarlane, D B; Macri, M M; Mader, W F; Majewski, S A; Malcles, J; Mallik, U; Mancinelli, G; Mandelkern, M A; Manoni, E; Marchiori, G; Margoni, M; Marks, J; Marsiske, H; Martin, E C; Martínez-Vidal, F; Mattison, T S; Mazur, M A; Mazzoni, M A; McKenna, J A; McMahon, T R; Mclachlin, S E; Meadows, B T; Menges, W; Merkel, J; Messner, R; Meyer, N T; Meyer, W T; Milanes, D A; Mir, L M; Mishra, K; Mohanty, G B; Monge, M R; Monorchio, D; Moore, T B; Morandin, M; Morganti, M; Morganti, S; Morii, M; Morris, J P; Muheim, F; Müller, D R; Nagel, M; Narsky, I; Nash, J A; Nauenberg, U; Neal, H; Negrini, M; Neri, N; Nicholson, H; Nikolich, M B; Nogowski, R; Nugent, I M; O'Grady, C P; Ocariz, J; Ofte, I; Olaiya, E O; Olivas, A; Olsen, J; Onuchin, A P; Orimoto, T J; Oyanguren, A; Ozcan, V E; Paar, H P; Pacetti, S; Palano, A; Palombo, F; Pan, B; Pan, Y; Panduro-Vazquez, W; Paoloni, E; Pappagallo, M; Park, W; Passaggio, S; Patel, P M; Patrignani, C; Patteri, P; Payne, D J; Pelizaeus, M; Pelliccioni, M; Perazzo, A; Perl, M; Peruzzi, I M

    2007-01-01

    We present measurements of the time-dependent CP-violating asymmetries in B0 --> K0S K0S K0S decays based on 384 million Y(4S) decays collected with the BABAR detector at the PEP-II asymmetric-energy B Factory at SLAC. We obtain the CP asymmetry parameters C = 0.02 +/- 0.21 +/- 0.05 and S = -0.71 +/- 0.24 +/- 0.04, where the first uncertainties are statistical and the second systematic. These results are consistent with standard model expectations.

  17. Measurement of the B+ --> rho+ pi0 Branching Fraction and Direct CP Asymmetry

    CERN Document Server

    Aubert, B; Abrams, G S; Adye, T; Ahmed, S; Alam, M S; Albert, J; Aleksan, R; Allen, M T; Allison, J; Altenburg, D D; Andreotti, M; Angelini, C; Anulli, F; Arnaud, N; Asgeirsson, D J; Aston, D; Azzolini, V; Baak, M A; Back, J J; Baldini-Ferroli, R; Band, H R; Banerjee, Sw; Bard, D J; Barlow, N R; Barlow, R J; Barrett, M; Bartoldus, R; Batignani, G; Battaglia, M; Bauer, J M; Bechtle, P; Beck, T W; Behera, P K; Bellini, F; Benayoun, M; Benelli, G; Berger, N; Bernard, D; Berryhill, J W; Best, D S; Bettarini, S; Bettoni, D; Bevan, A J; Bhimji, W; Bhuyan, B; Bianchi, F; Biasini, M; Biesiada, J; Blanc, F; Blaylock, G; Blinov, V E; Bloom, P C; Blount, N L; Bomben, M; Bondioli, M; Bonneaud, G R; Bosisio, L; Boutigny, D; Bowerman, D A; Boyd, J T; Bozzi, C; Brandenburg, G; Brandt, T; Brau, J E; Briand, H; Brown, C M; Brown, D N; Bruinsma, M; Brunet, S; Bucci, F; Buchanan, C; Bugg, W; Bukin, A D; Bula, R; Burchat, P R; Burke, J P; Button-Shafer, J; Buzzo, A; Bóna, M; Cahn, R N; Calabrese, R; Calcaterra, A; Calderini, G; Campagnari, C; Carpinelli, M; Cartaro, C; Cavallo, N; Cavoto, G; Cenci, R; Chai, X; Chaisanguanthum, K S; Chao, M; Charles, E; Charles, M J; Chauveau, J; Chavez, C A; Chen, A; Chen, C; Chen, E; Chen, J C; Chen, S; Chen, X; Chen, X R; Cheng, B; Cheng, C H; Chia, Y M; Cibinetto, G; Clark, P J; Claus, R; Cochran, J; Coleman, J P; Contri, R; Convery, M R; Corwin, L A; Cossutti, F; Cottingham, W N; Couderc, F; Covarelli, R; Cowan, G; Cowan, R; Crawley, H B; Cremaldi, L; Cunha, A; Curry, S; Côté, D; D'Orazio, A; Dahmes, B; Dallapiccola, C; Danielson, N; Dasu, S; Datta, M; Dauncey, P D; David, P; Davier, M; Davis, C L; De Nardo, Gallieno; De Sangro, R; Del Amo-Sánchez, P; Del Buono, L; Del Re, D; Della Ricca, G; Denig, A G; Di Lodovico, F; Di Marco, E; Dingfelder, J C; Dittongo, S; Dong, L; Dorfan, J; Druzhinin, V P; Dubitzky, R S; Dubois-Felsmann, G P; Dujmic, D; Dunwoodie, W; Dvoretskii, A; Ebert, M; Eckhart, E A; Eckmann, R; Edgar, C L; Edwards, A J; Egede, U; Eigen, G; Eisner, A M; Elmer, P; Emery, S; Ernst, J A; Eschenburg, V; Eschrich, I; Eyges, V; Fabozzi, F; Faccini, R; Fang, F; Feltresi, E; Ferrarotto, F; Ferroni, F; Field, R C; Finocchiaro, G; Flacco, C J; Flack, R L; Flächer, H U; Flood, K T; Ford, K E; Ford, W T; Forster, I J; Forti, F; Fortin, D; Foulkes, S D; Franek, B; Frey, R; Fritsch, M; Fry, J R; Fulsom, B G; Gabathuler, E; Gaidot, A; Gallo, F; Gamba, D; Gamet, R; Gan, K K; Ganzhur, S F; Gary, J W; Gaspero, M; Gatto, C; Gaz, A; George, K A; Gill, M S; Giorgi, M A; Gladney, L; Glanzman, T; Godang, R; Golubev, V B; Gowdy, S J; Gradl, W; Graham, M T; Graugès-Pous, E; Grenier, P; Gritsan, A V; Grosdidier, G; Groysman, Y; Hadavand, H K; Haire, M; Halyo, V; Hamano, K; Hamel de Monchenault, G; Hamon, O; Harrison, P F; Harrison, T J; Hart, A J; Hartfiel, B L; Hast, C; Hauke, A; Hawkes, C M; Hearty, C; Held, T; Hertzbach, S S; Heusch, C A; Hill, E J; Hirschauer, J F; Hitlin, D G; Hollar, J J; Hong, T M; Honscheid, K; Hopkins, D A; Hrynóva, T; Hufnagel, D; Hulsbergen, W D; Hutchcroft, D E; Höcker, A; Igonkina, O; Innes, W R; Izen, J M; Jackson, P D; Jackson, P S; Jacobsen, R G; Jain, V; Jasper, H; Jawahery, A; Jessop, C P; Judd, D; Kadyk, J A; Kagan, H; Karyotakis, Yu; Kass, R; Kelsey, M H; Kerth, L T; Khan, A; Kim, H; Kim, P; Kirkby, D; Klose, V; Knecht, N S; Koch, H; Kolb, J A; Kolomensky, Yu G; Kovalskyi, D; Kowalewski, R V; Kozanecki, W; Kreisel, A; Krishnamurthy, M; Kroeger, R; Kroseberg, J; Kukartsev, G; Kutter, P E; Kyberd, P; La Vaissière, C de; Lacker, H M; Lae, C K; Lafferty, G D; Lanceri, L; Lange, D J; Lankford, A J; Latham, T E; Latour, E; Lau, Y P; Lazzaro, A; Le Diberder, F R; Lees, J P; Legendre, M; Leith, D W G S; Lepeltier, V; Leruste, P; Lewandowski, B; Li Gioi, L; Li, S; Li, X; Lista, L; Liu, H; Lo Vetere, M; LoSecco, J M; Lockman, W S; Lombardo, V; Long, O; Lopez-March, N; Lou, X C; Lu, M; Luitz, S; Lund, P; Luppi, E; Lusiani, A; Lutz, A M; Lynch, G; Lynch, H L; Lü, C; Lüth, V; MacFarlane, D B; Macri, M M; Mader, W F; Majewski, S A; Malcles, J; Mallik, U; Mancinelli, G; Mandelkern, M A; Marchiori, G; Margoni, M; Marks, J; Marsiske, H; Martínez-Vidal, F; Mattison, T S; Mazur, M A; Mazzoni, M A; McKenna, J A; McMahon, T R; Mclachlin, S E; Meadows, B T; Mellado, B; Menges, W; Merkel, J; Messner, R; Meyer, N T; Meyer, W T; Mihályi, A; Mir, L M; Mishra, K; Mohanty, G B; Monge, M R; Monorchio, D; Moore, T B; Morandin, M; Morganti, M; Morganti, S; Morii, M; Muheim, F; Müller, D R; Nagel, M; Naisbit, M T; Narsky, I; Nash, J A; Nauenberg, U; Neal, H; Negrini, M; Neri, N; Nesom, G; Nicholson, H; Nikolich, M B; Nogowski, R; Nugent, I M; O'Grady, C P; Ocariz, J; Ofte, I; Olaiya, E O; Olivas, A; Olsen, J; Onuchin, A P; Orimoto, T J; Oyanguren, A; Ozcan, V E; Paar, H P; Pacetti, S; Palano, A; Palombo, F; Pan, B; Pan, Y; Panduro-Vazquez, W; Paoloni, E; Paolucci, P

    2007-01-01

    We present improved measurements of the branching fraction and CP asymmetry for the process B+ --> rho+ pi0. The data sample corresponding to 211/fb comprises 232 million Y(4S)-->BBbar decays collected with the BABAR detector at the PEP-II asymmetric B Factory at SLAC. The yield and CP asymmetry are measured using an extended maximum likelihood fitting method. The branching fraction and CP asymmetry are found to be BR(B+ --> rho+ pi0)= [10.2 +- 1.4(stat) +- 0.9(syst)] x 10^-6 and Acp (B+ --> rho+ pi0) = -0.01 +- 0.13(stat) +- 0.02(syst).

  18. Comportamiento asertivo y adaptación social : adaptación de una escala de comportamiento asertivo (CABS) para escolares de enseñanza primaria (6-12 años)

    OpenAIRE

    Peña, V. de la; Hernández Granda, Eva; Rodríguez Díaz, Francisco Javier

    2003-01-01

    Este trabajo es un estudio de la Escala de Comportamiento Asertivo (CABS) en una muestra de alumnos de Enseñanza Primaria de la Comunidad Autónoma del Principado de Asturias ( 2º Curso: N=119 y 4º y 6º: N=260). El objetivo es lograr una escala breve para discriminar el estilo asertivo frente a los no asertivos en las edades iniciales de escolarización. Para ello hemos eliminado los ítems redundantes (que producían una matriz de covarianzas con determinante 0) y, posteriormente, se ha evaluado...

  19. 環太平洋地域は運命共同体である

    OpenAIRE

    木村, 光伸

    2014-01-01

      El gran terremoto que azoto toda la costa del Pacifico de la region de Tohoku a las 2:16 de la tarde del 11 de marzo de 2011, creo colosales olas de maremoto “tsunami” y causo grandes danos humanos y patrimoniales a los vecinos del litoral. Es el Gran Terremoto de Japon Oriental. Este terremoto y tsunami dieron golpes directos a la central nuclear Fukushima Dai-ichi de la empresa Tokyo Electric Power Company (TEPCO) y arrasaron los reactores 1, 2, 3 y 4 de la planta de generadores nucleares...

  20. 从不同角度看不等式的证明

    Institute of Scientific and Technical Information of China (English)

    郭松

    2005-01-01

    不等式的证明方法众多,使许多同学感到无所适从,以下笔者通过一常见例题加以剖析【例】已知1x+1y=1,(x、y∈ R*)求证x+y≥4分析1:已知条件是分式,求证的是整式.如果分析这个矛盾可得以下方法方法1:x+y =(x+y)(1x+1y)=2+yx+xy≥4方法2:∵1=1x+1y≥21xy∴xy≥2又∵x+y≥2xy∴x+y≥4分析2:也许有人这样证明x +y+1x+1y=(x+1x) +(y+1y)≥2.证明错误的原因在于等号两边不能同时分析这个矛盾可得以下方法方法3:x+y +4x+4y=(x+4x) +(y +4y) ≥8∵4x+4y=4∴x+y≥4方法4:4x+4y+1x+1y=(4x+1x) +(4y+1y) ≥2∴x+y≥4分析3:如果从函数角度分析x+y≥4,有两个变量,而已知1x+1y=1,则有方法5:∵1x+1y=1∴x =y -y1∴x+y =y -y1+y=y y-21=(y -1)(yy +1) +1=y +1+y 1-1=(y -1) +y 1-1+2≥4方法6:设x+y =t得x =t -y∵1x+1y=1∴t =y(t -y)y2-ty +t =0(-t)2-4t >0t ...

  1. Existence of Solutions of Nonlinear Two-Point Boundary Value Problems for Fourth-Order Nonlinear Differential Equation%四阶常微分方程两点边值问题解的存在性

    Institute of Scientific and Technical Information of China (English)

    高永馨

    2003-01-01

    利用上下解的方法,讨论了非线性四阶常微分方程y(4)=f(t,y,y′,y″,y′″)满足条件g0(y(a),y′(a))=0,g1(y′(a),y″(a))=0,g2(y″(a),y′"(a))=0h(y(c),y′(c),y″(c),y′(c))=0的非线性两点边值问题解的存在性,其中函数f,gi和h是具有一定单调性质的连续函数.

  2. e do espectro de gotas de bicos hidráulicos

    Directory of Open Access Journals (Sweden)

    José Luís Aguiar Figueiredo

    2007-01-01

    Full Text Available Fueron realizados ensayos de laboratorio con las boquillas de las series LD y API y con boquillas tipo cono vacío JD 12P, caracterizando el espectro de la población de gotas obtenidas y la uniformidad de distribución tranversal del líquido pulverizado. La mejor uniformidad de distribución fue obtenida con boquillas de la serie API,. Con presiones entre 2 y 4 bar en la altura de 50 cm sobre el objetivo. Menores tasas de aplicación incidieron en gotas de menor tamaño y mejor cobertura (imp/cm2.

  3. Una manera diferente de abordar la sexualidad, la contracepción y la prevención de infecciones de transmisión sexual desde la escuela en la Costa da Morte

    OpenAIRE

    Luces Lago, Ana María; Porto Esteiro, Marta; Mosquera Pan, Lucía; Tizón Bouza, Eva

    2015-01-01

    Introducción: La actividad sexual en edad precoz se relaciona con conductas de riesgo que pueden dar lugar a embarazos no deseados (END) o infecciones de trasmisión sexual (ITS). Los centros de enseñanza son los espacios idóneos para realizar actividades educativas en materia de sexualidad. Nuestro estudio tiene como objetivos: averiguar los conocimientos sobre sexualidad, anticoncepción e ITS de los alumnos de 2º,3º y 4º de la ESO, generar conocimientos y actitudes adecuadas en torno a la se...

  4. High dose botulinum toxin A for the treatment of lower extremity hypertonicity in children with cerebral palsy

    OpenAIRE

    Willis, Allison W.; Crowner, Beth; Brunstrom, Janice E; Kissel, Abigail; Racette, Brad A.

    2007-01-01

    The aim of this study was to determine the safety profile of high dose (15–25 units/kg) of botulinum toxin A (BTX-A) in children with cerebral palsy (CP) and increased lower extremity muscle tone. We performed a retrospective review of 929 patient encounters at the Movement Disorders Center at Washington University. A total of 261 patients (105 females; 156 males) were treated during these visits, ages 6 months to 21 years (mean 8y 4mo [SD 4y 8mo]). Ambulatory ability at the time of BTX-A inj...

  5. Formación de Subtipos de Niños con Problemas Escolares de Aprendizaje a Partir de la Evaluación Neuropsicológica, Capacidades Cognitivas y Comportamiento

    OpenAIRE

    Teresa Álvarez Arenal; Pablo A. Conde-Guzón

    2009-01-01

    En el presente trabajo se han diferenciado subtipos de niños en edad escolar que no presentan alteración neurológica cerebral demostrable y manifiestan problemas de aprendizaje. Se han diferenciado desde la perspectiva neuropsicológica, cognitiva y del comportamiento. Las pruebas de evaluación utilizadas son: La Batería Luria-DNI, el WISC-R y las Escalas de Comportamiento Infantil (ECI). Hemos obtenido 4 subtipos: (1) Trastorno disléxico, (2) Déficit generalizado, (3) Déficit de atención y (4...

  6. Estabilidad oxidativa de huevos enriquecidos con ácidos grasos poliinsaturados omega 3, frente a antioxidantes naturales Estabilidade oxidativa de ovos enriquecidos com ácidos graxos poliinsaturados ômega 3, frente a antioxidantes naturais

    OpenAIRE

    María Elena Bernal Gómez; Cássio Xavier de Mendonça-Junior; Jorge Mancini-Filho

    2003-01-01

    Fueron alimentadas 192 gallinas ponedoras de 22 semanas de edad, de linaje comercial Babcock, durante 30 días con dietas de 0 (cero) y 12,7% de semilla de linaza molida que corresponde a 0 y 5% de aceite de linaza, respectivamente. Fueron definidos 8 tratamientos: 4 grupos con 12,7% de semilla de linaza (control/sin antioxidante; BHA+BHT, 100+100 ppm; orégano, 200 ppm; romero, 200 ppm) y 4 grupos sin semilla de linaza, pero utilizando los mismos antioxidantes. Este estudio fue realizado con e...

  7. Control no lineal robusto de una maqueta de helicóptero con rotores de velocidad variable

    OpenAIRE

    R. Rubio, Francisco; Vivas Venegas, Carlos; G. Ortega, Manuel; López-Martínez, Manuel

    2007-01-01

    En este artículo se presenta el diseño de un controlador robusto no lineal para un helicóptero de laboratorio. El equipo se caracteriza por tener los ángulos de ataque de las hélices de los rotores fijos y se controla mediante el cambio de velocidad de los rotores a través de dos motores de accionamiento directo. El sistema resultante es multivariable (de 2 entradas y 4 salidas) altamente no lineal y fuertemente acoplado. La aplicación presentada en este artículo considera el diseño de un sis...

  8. 鲜切马铃薯贮藏期间微生物生长模型的建立%The Establishment of Microorganism Growth Model of Fresh-cut Potato During Storage Period

    Institute of Scientific and Technical Information of China (English)

    宋留丽; 郁志芳

    2013-01-01

    The growth trend of bacteria and change of sensory quality of fresh-cut potato which had been treated by preserving during the storage at 0 and 15℃C was studied.The results showed that the Gompertz model y</