WorldWideScience

Sample records for agglomerans em diversas

  1. Desenvolvimento de Pantoea agglomerans em diversas temperaturas, pH e concentrações de carboxi-metil-celulose e o seu impacto no controle de Rosellinia necatrix Development of Pantoea agglomerans under different temperatures, pH and carboximethyl cellulose rates and their effect on Rosellinia necatrix control

    Directory of Open Access Journals (Sweden)

    Valdirene Camatti-Sartori

    2008-02-01

    Full Text Available Rosellinia necatrix Prill induz a podridão branca da raiz da macieira, doença que causa severa perda em pomares localizados no sul do Brasil. O manejo da doença é principalmente preventivo e inclui o uso de porta-enxertos resistentes e a fumigação do solo. A proteção das mudas de macieiras antes do plantio com um isolado antagonista de Pantoea agglomerans foi recentemente proposto para reduzir a incidência da doença. Os objetivos desta pesquisa foram caracterizar o relacionamento entre o patógeno e a bactéria antagonista; a produção de metabólitos biológicamente ativos pelo isolado bacteriano e a sua ação sobre o patógeno; o efeito do pH, da temperatura e de carboxi-metil-celulose (CMC sobre o crescimento do antagonista e do patógeno, isolados ou não e no controle da doença. Os resultados demonstraram que o crescimento de P. agglomerans foi maior em pH 5,5 e 6,0 e nas temperaturas de 20 °C e 30 °C. O crescimento micelial de R. necatrix foi inibido em meio de cultura e previamente colonizado pelo antagonista e com CMC nas concentrações de 0,25 e 0,5 %. A proteção das macieiras da infecção por R. necatrix foi observada quando utilizadas as concentrações de 10(7; 10(8 e 10(9 cel/mL e nas diferentes concentrações de CMC. Maior desenvolvimento radicular das macieiras foi constatado em todas as concentrações de CMC quando usada a concentração de 10(9 cel/mL. A formulação de P. agglomerans com CMC tornará possível a incorporação desta estratégia de controle ao manejo integrado da podridão branca das raízes da macieira no Sul do Brasil.Rosellinia necatrix Prill induces the white root rot of apple, a disease that causes severe losses in orchards located in Southern Brazil. The management of the disease is mostly preventative and include usage of less susceptible rootstocks and soil fumigation. Protection of nursery apple trees before planting with an antagonistic bacterium Pantoea agglomerans was recently

  2. Biological control of fire blight in pear orchards with a formulation of Pantoea agglomerans strain Eh 24 Controle biológico de fire blight em pereiras empregando uma formulação de Pantoea agglomerans Eh 24

    Directory of Open Access Journals (Sweden)

    Hatice Özaktan

    2004-09-01

    Full Text Available Biological control by using epiphytic bacteria against Erwinia amylovora has been considered as an alternative method for controlling the disease. Talc-based formulation of Pantoea agglomerans strain Eh-24 was applied at 30% and 100% bloom on two pear orchards which were selected from different locations in the Aegean Region in Turkey. Pear orchard trials were replicated for two years (1999 and 2000 in each place. Talc-based formulation of P. agglomerans strain Eh-24 was sprayed on pear trees which were naturally infected with E. amylovora. In the orchard trials conducted in 1999 and 2000, talc-based formulation of P. agglomerans strain Eh-24 reduced the percentage of blighted blossoms on pear orchards by 63% to 76%, approximately. Copper oxychloride+maneb was less effective in reducing the incidence of blossom infection by E. amylovora in each pear orchard than the bioformulation treatment. P. agglomerans strain Eh-24 labelled with StrR+ was applied at 30% and 100% bloom to monitor the colonization and population dynamics of P. agglomerans on pear blossoms. The population size of P. agglomerans strain Eh-24 strR+ on pear blossoms increased from 2x10(4 to 1.3x10(6 cfu per blossom over 18 days.Controle biológico de Erwinia amylovora através do uso de bactérias epifíticas tem sido considerado um método alternativo para o controle de "fire blight". Uma formulação de Pantoea agglomerans Eh 24 em talco foi utilizada em pereiras a 30% e a 100% de floração, em duas plantações selecionadas na região Aegean da Turquia. Os experimentos foram repetidos duas vezes (1999 e 2000 em cada plantação. A formulação de P. agglomerans foi aspergida nas pereiras naturalmente infectadas com E. amylovora. Nos experimentos de 1999 e 2000, a redução da porcentagem de ocorrência de "fire blight" foi reduzida aproximadamente em 63% e em 76%, respectivamente. Oxicloreto de cobre + maneb foi menos eficiente na redução da infecção por E. amylovora do

  3. O dinheiro e suas diversas faces nas eleições municipais em Pernambuco

    Directory of Open Access Journals (Sweden)

    Jorge Mattar Villela

    2005-04-01

    Full Text Available O artigo pretende mostrar como o dinheiro utilizado nas eleições municipais no Sertão de Pernambuco atua com um desterritorializador das relações sociais previamente existentes, ainda que apenas em alguns casos. Ele é um signo de mercantilização do processo eleitoral que encerra em si mesmo as transações de longo prazo envolvendo o empenho da palavra, das promessas e dos blocos de dívida e crédito, tanto de eleitores quanto de candidatos. Mas o dinheiro em sua forma pura pode ser desmonetarizado pelas próprias relações de dívida e crédito no interior da circulação de recursos-voto por recursos-favor. Por outro lado, bens e serviços que implicam apenas indiretamente o dinheiro podem sofrer um processo de monetarização, dependendo da forma como são aplicados.The article aims to show how the money used in the municipal elections in the Pernambuco Outback acts as a deterritorializor of previously existing social relations, albeit in a few cases only. It is a sign of commercializing the electoral process which encompasses the long-term processes involving the pledges, promises and blocks of debt and credit, both those of voters and candidates. But money in its pure form may be demonetarized through the relations of debt and credit themselves within the circulation of voting-resources by favour-resources. On the other hand, goods and services which only indirectly involve money may become monetarized, depending on the form in which they are applied.

  4. Estudo longitudinal do desenvolvimento da comunicação em diversas economias mundiais.

    Directory of Open Access Journals (Sweden)

    Emílio José Montero Arruda Filho

    2012-08-01

    Full Text Available O desenvolvimento do setor de comunicação de um país possui relações com o seu desenvolvimento econômico e é possível demonstrar essa conexão estatisticamente, como tem sido feito por pesquisadores de instituições como a ITU (International Telecommunications Union. Como esses dados estão apenas apresentados na forma simples e não levam em conta fatores de interconexão, é importante desenvolver pesquisas que avaliem a conexão entre variáveis relevantes no campo. Este trabalho visa analisar os fatores de desenvolvimento tecnológico de comunicação que melhor descrevem o posicionamento econômico de um país, integrando a usabilidade dos serviços de comunicação com o status econômico. Foram utilizados dados de 56 países, escolhidos aleatoriamente. Uma pesquisa na forma de datamining foi realizada, a qual analisou os dados existentes já previamente coletados por instituições de pesquisa tais como ITU, World Bank, e outras. Cada fator de usabilidade, preferência e serviço analisado é apontado como um reflexo de outros serviços e usos anteriormente desenvolvidos. Parte da literatura sobre esse tópico (ARRUDA FILHO; CASSIA; MARINO, 2008; CHERRY, 2007; DAVIES; HARDT; KELLY, 2004 argument que o desenvolvimento econômico de certos países existe como resultado de um aumento do desenvolvimento tecnológico, porém outras demonstram ou informam o contrário. Este artigo apresenta essas relações em certos países, escolhidos pela quantidade de informação disponível considerando sua posição de desenvolvimento global, relacionando diretamente o comportamento de crescimento ou redução no uso das tecnologias, tais como telefone e internet. Este trabalho coletou dados de duas fontes mundiais de informação dos setores mencionados: a International Telecommunications Union (ITU e o World Bank. Utilizaram-se as técnicas estatísticas de regressão e análise de variância para a análise de dados de 56 países ao longo de

  5. Amido resistente em diversas fontes não convencionais de amido

    Directory of Open Access Journals (Sweden)

    C. Fontinha

    2016-02-01

    Full Text Available Amido resistente (AR é aquele que não é digerido no intestino delgado, conduzindo a efeitos benéficos para a saúde, tal como a fibra dietética faz. Procedeu-se à determinação do AR em farinhas e amidos extraídos de duas variedades de castanha (Castanea sativa Mill, Longal e Martainha, da bolota de azinheira (Quercus rotundifolia e da glande do sobreiro (Quercus suber. As farinhas foram obtidas por moenda dos frutos após secagem a diferentes temperaturas, 40º C, 50º C, 60ºC e 70ºC. Posteriormente, procedeu-se ao isolamento dos amidos das farinhas, através da aplicação de dois métodos de extracção: um físico-químico e outro enzimático. Verificou-se que os teores de AR mais elevados foram os apresentados pelas farinhas dos frutos secos a 60ºC, sendo de 46,8%, 36,5%, 39,9% e 43,9%, respectivamente para as castanhas das variedades Longal e Martainha, para a bolota e para a glande. O método de extracção que apresentou valores mais elevados de AR para os amidos extraídos dos diferentes materiais foi o método físico-químico. Tudo indica que a secagem dos frutos a 60ºC e a aplicação do método de extracção do amido pelo processo físico-químico nas farinhas dos frutos secos a esta temperatura são aqueles que melhor preservam a estrutura dos amidos.

  6. Membrana amniótica: fonte alternativa de células-tronco mesenquimais em diversas espécies animais

    OpenAIRE

    Faita,Thalita; Silva,Verônica N.; Sattin,William R.; Pinheiro,Alessandra O.; Ambrósio,Carlos E.

    2016-01-01

    Resumo: O crescente interesse do uso de células-tronco (CTs) na medicina regenerativa, estão baseadas na sua capacidade de autorrenovação, reparação tecidual e imunomodulação. A terapia utilizando células-tronco é uma ciência em formação considerada uma alternativa terapêutica promissora no tratamento de diversas patologias degenerativas, hereditárias e inflamatórias. A busca por fontes alternativas de CTs que possuam características como fácil obtenção e isolamento, tem levado ao estudo dos ...

  7. Membrana amniótica: fonte alternativa de células-tronco mesenquimais em diversas espécies animais

    Directory of Open Access Journals (Sweden)

    Thalita Faita

    2016-06-01

    Full Text Available Resumo: O crescente interesse do uso de células-tronco (CTs na medicina regenerativa, estão baseadas na sua capacidade de autorrenovação, reparação tecidual e imunomodulação. A terapia utilizando células-tronco é uma ciência em formação considerada uma alternativa terapêutica promissora no tratamento de diversas patologias degenerativas, hereditárias e inflamatórias. A busca por fontes alternativas de CTs que possuam características como fácil obtenção e isolamento, tem levado ao estudo dos mais variados tecidos, onde se inclui os anexos embrionários como a membrana amniótica. Porém, para sua efetiva aplicação terapêutica são necessários estudos de suas características "in vitro", além de testes pré-clínicos e clínicos. Com isso, o objetivo deste trabalho é realizar uma revisão bibliográfica sobre a caracterização e aplicação clínica das células-tronco da membrana amniótica em diversas espécies de animais domésticos.

  8. Caracterização estrutural e da capacidade de absorção de água em filmes finos de quitosana processados em diversas concentrações

    Directory of Open Access Journals (Sweden)

    Assis Odilio B. G.

    2003-01-01

    Full Text Available Filmes finos de quitosana (média massa molecular foram produzidos a partir de soluções precursoras em diversas concentrações e caracterizados com respeito à estrutura e grau de absorção de água. Os filmes foram obtidos por adsorção sobre lâminas de vidro previamente funcionalizadas (técnica de automontagem. Análises por microscopia eletrônica de varredura e de força atômica revelaram espessuras fracamente relacionadas à concentração do polissacarídeo, com espessuras não superiores a 25-30 nm e caracterizados por estrutura de nano poros. A afinidade por água indica, por sua vez, forte dependência com um comportamento do tipo exponencial com a concentração de quitosana. Testes comparativos em solução tampão também foram realizados demonstrando comportamento diverso ao obtido com água.

  9. Prevalência de demência em diversas regiões do mundo: Análise dos estudos epidemiológicos de 1994 a 2000

    Directory of Open Access Journals (Sweden)

    Lopes Marcos A.

    2002-01-01

    Full Text Available OBJETIVO: Analisar os estudos de prevalência de demência, no período de 1994 a 2000. MÉTODOS: Foram utilizadas as bases de dados Medline e Lilacs e os seguintes critérios de inclusão: constituir artigo original, estimar prevalência de demência e utilizar amostra comunitária. RESULTADOS: Foram avaliados 38 artigos, originários de todos os continentes. A idade exerceu importante influência sobre os resultados, com taxas médias de prevalência de demência que variaram de 1,17% na faixa de 65-69 anos, a 54,83% na faixa acima de 95 anos. Houve predomínio do sexo feminino em 75% dos estudos avaliados. A relação doença de Alzheimer/demência vascular foi maior na América do Sul e menor na Ásia. DISCUSSÃO: Ocorreram avanços metodológicos, sobretudo com relação à maior homogeneidade dos critérios diagnósticos. O efeito da idade sobre o aumento das taxas de prevalência foi pronunciado, principalmente até os 90 anos de idade.

  10. Corpos de inclusão citoplasmática: estudo em diversas doenças e revisão da literatura Inclusion cytoplasmic bodies: a study in several diseases and a literature review

    Directory of Open Access Journals (Sweden)

    Rosana Herminia Scola

    1996-06-01

    Full Text Available Estudamos 16 casos entre 1400 biópsias musculares que apresentavam vacúolos marginados, cujo aspecto histológico sugeria corpos de inclusão citoplasmáticos. Procuramos correlacionar os dados clínicos, laboratoriais e histopatológicos, a fim de determinar a especificidade dos corpos de inclusão citoplasmáticos para determinadas doenças. A creatinaquinase mostrou-se elevada em 10 casos. A eletromiografia foi anormal em todos os casos. A histoquímica muscular em 5 casos revelou uma miopatia, em 7 padrão misto, em dois desinervação e em 2 casos miopatia inflamatória. A microscopia eletrônica demonstrou a presença de filamentos em 8 casos (nucleares, dispersos no citoplasma ou na região subsarcolemal. Os pacientes foram classificados conforme a história clínica, hereditariedade, dados laboratoriais, eletrofisiológicos, histoquímicos e microscopia eletrônica Encontramos miosite com corpos de inclusão citoplasmática (4 casos, atrofia muscular espinhal juvenil (6 casos, miopatias distais (3 casos, distrofia de cinturas pélvica e escapular (2 casos e polineuropatia periférica (1 caso. Apresentamos revisão sobre a patogenia, formação e possível etiologia dos vacúolos marginados e sua relação com as diversas entidades em que foram detectados, sugerindo que não são específicos para uma única doença.Among 1400 muscle biopsies, we studied 16 cases with rimmed vacuoles, whose histology suggests cytoplasm inclusion bodies. We tried to correlate the clinical, laboratory and histopatological data in order to verify the specificity of cytoplasm inclusion bodies to certain diseases. The creatinekinase was increased in 10 cases. In all cases electromyography was abnormal. Muscle histochemistry revealed myopathy in 5 cases, mixed pattern in 7, denervation in 2 and in 2 cases, inflammatory myopathy. Electron microscopy showed the presence of filaments in 8 cases (nuclear, disseminated in cytoplasm or in the subsarcolemmal region

  11. Desenvolvimento de PVC reforçado com resíduos de Pinus para substituir madeira convencional em diversas aplicações Development of PVC/wood composites for the replacement of conventional wood products

    Directory of Open Access Journals (Sweden)

    Antonio Rodolfo Jr.

    2006-03-01

    Full Text Available Este trabalho avalia a viabilidade técnica da obtenção de compósitos lignocelulósicos de PVC, utilizando-se resíduo de Pinus elliottii e Pinus taeda como carga reforçativa. Foi desenvolvido um processo simples e economicamente viável de tratamento de resíduos industriais desta madeira, processo este baseado na secagem e revestimento das partículas com lubrificantes funcionais e agentes de acoplamento utilizados como aditivos na indústria do PVC, bem como no uso de equipamentos tradicionais da indústria de processamento deste termoplástico. Foram avaliados os efeitos da incorporação da farinha de madeira em concentrações variáveis e do tipo de agente de tratamento superficial utilizado na processabilidade do composto de PVC, bem como em propriedades finais do compósito. Os resultados mostram que o desenvolvimento deste tipo de material compósito é uma alternativa viável para a substituição da madeira convencional em diversas aplicações.This work evaluates the technical viability of lignocellulosic vinyl composites, using residues of Pinus elliottii and Pinus taeda as the reinforcement fiber. A simple and economically viable process for the treatment of these industrial residues was developed. The process includes sieving, drying and treating the wood particles. Treatment is made with functional lubricants and coupling agents used as additives in the PVC industry. Extrusion was performed using traditional equipment available in the Brazilian PVC processing industry. The effect on the processability of the variable concentrations of the residues incorporated and the type of agent used for the treatment had been evaluated, as well as in the final properties of the composite. The results show that the development of this kind of composite material is a viable alternative for the substitution of conventional wood in diverse applications.

  12. Spondylodiscitis Caused by Enterobacter agglomerans.

    Science.gov (United States)

    Jayaweera, Jayaweera Arachchige Asela Sampath; Kothalawala, Mahen; Devakanthan, Balachandran; Arunan, Sinnappoo; Galgamuwa, Dinithi; Rathnayake, Manori

    2016-01-01

    All over the globe, the incidence of vertebral infection is rising. Nowadays, compared to tuberculous variety, pyogenic spondylodiscitis incidence is high. The increase in the susceptible population and improved diagnostics summatively contributed to this. In clinical grounds, differentiation of pyogenic and tuberculous spondylodiscitis is well defined. Enterobacter agglomerans is a hospital contaminant and associated with infections in immunocompromised individuals and intravenous lines. It causes a wide array of infections. Enterobacter agglomerans spondylodiscitis is unusual and there are, around the globe, only less than 31 suspected cases that have been previously reported. Enterobacter agglomerans histology mimics tuberculous rather than pyogenic spondylodiscitis. A 65-year-old farming lady, while being in hospital, developed sudden onset spastic paraparesis with hyperreflexia. Later blood culture revealed Enterobacter agglomerans with 41-hour incubation in 99.9% probability from Ramel identification system. Her initial ESR was 120 mm/first hour. Isolate was susceptible to ciprofloxacin and intravenous followed with oral therapy shows a drastic ESR fall and improved clinical response. Differentiation of tuberculous and pyogenic spondylodiscitis is very much important in management point of view. Therefore, blood culture has a role in diagnosis of spondylodiscitis. ESR can be used as important inflammatory marker in monitoring the response to treatment. Retrospectively, ESR would aid in reaching a definitive diagnosis.

  13. Cholecystitis associated with Enterobacter agglomerans.

    Science.gov (United States)

    Nilakul, C; Sukroongreung, S; Fucharoen, S; Vathanophas, V

    1984-06-01

    A case report of chronic cholecystitis due to Enterobacter agglomerans, occurred in a 54-year old female with homozygous beta-thalassemia. The patient responded successfully to cholecystectomy and sulfamethoxazole + trimetroprim therapy. The source of the infection was not known, however, cystic duct obstruction and immune deficit were thought to be the predisposing causes.

  14. Spondylodiscitis Caused by Enterobacter agglomerans

    Directory of Open Access Journals (Sweden)

    Jayaweera Arachchige Asela Sampath Jayaweera

    2016-01-01

    Full Text Available All over the globe, the incidence of vertebral infection is rising. Nowadays, compared to tuberculous variety, pyogenic spondylodiscitis incidence is high. The increase in the susceptible population and improved diagnostics summatively contributed to this. In clinical grounds, differentiation of pyogenic and tuberculous spondylodiscitis is well defined. Enterobacter agglomerans is a hospital contaminant and associated with infections in immunocompromised individuals and intravenous lines. It causes a wide array of infections. Enterobacter agglomerans spondylodiscitis is unusual and there are, around the globe, only less than 31 suspected cases that have been previously reported. Enterobacter agglomerans histology mimics tuberculous rather than pyogenic spondylodiscitis. A 65-year-old farming lady, while being in hospital, developed sudden onset spastic paraparesis with hyperreflexia. Later blood culture revealed Enterobacter agglomerans with 41-hour incubation in 99.9% probability from Ramel identification system. Her initial ESR was 120 mm/first hour. Isolate was susceptible to ciprofloxacin and intravenous followed with oral therapy shows a drastic ESR fall and improved clinical response. Differentiation of tuberculous and pyogenic spondylodiscitis is very much important in management point of view. Therefore, blood culture has a role in diagnosis of spondylodiscitis. ESR can be used as important inflammatory marker in monitoring the response to treatment. Retrospectively, ESR would aid in reaching a definitive diagnosis.

  15. Spondylodiscitis Caused by Enterobacter agglomerans

    OpenAIRE

    Jayaweera, Jayaweera Arachchige Asela Sampath; Kothalawala, Mahen; Devakanthan, Balachandran; Arunan, Sinnappoo; Galgamuwa, Dinithi; Rathnayake, Manori

    2016-01-01

    All over the globe, the incidence of vertebral infection is rising. Nowadays, compared to tuberculous variety, pyogenic spondylodiscitis incidence is high. The increase in the susceptible population and improved diagnostics summatively contributed to this. In clinical grounds, differentiation of pyogenic and tuberculous spondylodiscitis is well defined. Enterobacter agglomerans is a hospital contaminant and associated with infections in immunocompromised individuals and intravenous lines. It ...

  16. Existe diferença na contratilidade da musculatura do assoalho pélvico feminino em diversas posições? Is there any difference between female pelvic floor muscle contractility in different positions?

    Directory of Open Access Journals (Sweden)

    Mariana Tirolli Rett

    2005-01-01

    Full Text Available OBJETIVO: avaliar e comparar resultados da eletromiografia de superfície do assoalho pélvico feminino em diversas posições (decúbito dorsal, na posição sentada e ortostática. MÉTODOS: foram avaliadas 26 mulheres submetidas a um protocolo de exercícios para o fortalecimento do assoalho pélvico como tratamento da incontinência urinária de esforço por hipermobilidade do colo vesical. Utilizou-se sensor intravaginal conectado ao equipamento Myotrac 3G TM e a avaliação consistia em: 60 segundos iniciais de repouso, cinco contrações fásicas, uma contração tônica de 10 segundos e outra de 20 segundos. As amplitudes destas contrações foram obtidas pela diferença entre a amplitude final da contração menos a amplitude de repouso (em µV e foram comparadas com o uso do teste de Wilcoxon para amostras pareadas (pPURPOSE: to evaluate and compare results of female pelvic floor surface electromyography in different positions: lying, sitting and standing. METHODS: twenty-six women with the diagnosis of stress urinary incontinence treated with a protocol of exercises to strengthen the pelvic floor muscle were evaluated. Pelvic floor surface electromyography was performed with an intravaginal sensor connected to Myotrac 3G TM equipment, as follows: initial rest of 60 s, five phasic contractions, one 10-s tonic contraction and one 20-s tonic contraction. The amplitudes were obtained from the difference between the final contraction amplitude and the amplitude at rest (in µV. Wilcoxon test was applied for nonparametric data (p value <0.05. RESULTS: the amplitudes of contractions were higher in the lying position, decreasing in the sitting and standing positions. In the lying position, the median values of phasic and tonic contractions were 23.5 (5-73, 18.0 (3-58 and 17.0 (2-48, respectively. In the sitting position, they were 20.0 (2-69, 16.0 (0-58 and 15.5 (1-48. In the standing position they were 16.5 (3-67, 12.5 (2-54 and 13.5 (2

  17. [Characterization of Pantoea agglomerans lipopolysaccharides].

    Science.gov (United States)

    Varbanets, L D; Brovarskaya, O S; Bulygina, T N; Garkavaya, E G; Zhitkevich, N V

    2014-01-01

    Lipopolysaccharides (LPS) from seven Pantoea agglomerans strains isolated from various plants were purified and chemically identified. LPS of the studied P. agglomerans strains were heterogeneous in monosaccharide composition. Thus, the LPS of P. agglomerans 8606 differed considerably from the LPSs of other strains, containing mannose as the predominant monosaccharide (69.8%), as well as ribose (15.1%) and xylose (12.6%), while the content of rhamnose, one of the predominant monosaccharides in other LPS samples, was 2.5%. Analysis of the fatty acid composition revealed the presence of C12-C16 acids. In lipids A of all the studied strains, 3-OH-C14:0 was the predominant acid (31.7 to 39.1%, depending on the strain). C12:0 (8.2 to 31.5%), C14:0 (12.9 to 30.8%), and C16:0 acids (3.4 to 16.9%) were also revealed. The studied P. agglomerans strains fell into three groups according to their fatty acid composition. The differences stemmed from the presence or absence of two fatty acids, 2-OH-C14:0 and C16:1. Ouchterlony double immunodiffusion in agar revealed that all the LPS under study exhibited antigenic activity in homologous systems. The results of serological cross reactions indicated immunochemical heterogeneity of the species P. agglomerans. Comparative investigation of the complex of parameters of peripheral blood cells from a healthy donor before and after treatment with LPS solutions showed that the values of no parameters exceeded the normal range.

  18. Enterobacter agglomerans--associated cotton fever.

    Science.gov (United States)

    Ferguson, R; Feeney, C; Chirurgi, V A

    1993-10-25

    Cotton fever is usually a benign febrile, leukocytic syndrome of unknown etiology seen in intravenous narcotic abusers. Cotton and cotton plants are heavily colonized with Enterobacter agglomerans. We report a case of cotton fever associated with E agglomerans in which the organism was first isolated from the patient's blood and secondarily from cotton that he had used to filter heroin. Enterobacter agglomerans is with most probability the causal agent of cotton fever. Patients presenting with the classic history should have blood cultures performed and should be started on a regimen of empiric antibiotic therapy.

  19. Septic arthritis caused by Enterobacter agglomerans.

    Science.gov (United States)

    Flatauer, F E; Khan, M A

    1978-05-01

    A case of septic arthritis was caused by Enterobacter agglomerans, an association that to our knowledge has not been described previously. The right knee joint of a previously healthy 11-year-old boy became infected when the organism was introduced through the overlying skin by a contaminated wooden splinter. Conservative management with antibiotic therapy and repeated arthrocenteses was successful. Enterobacter agglomerans is a known plant pathogen, and a relationship between human infections caused by this organism and contact with plants is well recognized. The patient described here demonstrates that, when given a suitable set of environmental circumstances, E agglomerans can cause infection in man, including septic arthritis.

  20. Crescimento dos frutos de laranjeira 'Salustiana' situados em ramos anelados com diversas relações de folhas/frutos Growth of the fruits of 'Salustiana' sweet orange located in girdled shoots with several leaves to fruit ratios

    Directory of Open Access Journals (Sweden)

    Dalmo Lopes de Siqueira

    2007-08-01

    Full Text Available A relação entre a área foliar e o crescimento dos frutos é um tema que freqüentemente recebe a atenção dos pesquisadores, por influenciar diretamente na produtividade das plantas e na qualidade dos frutos. Neste trabalho, avaliou-se o efeito da área foliar sobre o crescimento dos frutos da laranjeira 'Salustiana'. Foram utilizados ramos com 12 meses de idade e com apenas um fruto terminal. Os ramos foram anelados visando a manter diversas relações de folhas/fruto. Avaliaram-se, semanalmente, o crescimento dos frutos e os teores de amido presentes nas folhas durante um período de 42 dias. O crescimento dos frutos, avaliado na "fase de crescimento II", dependeu da área foliar disponível por fruto, sendo que 30 folhas foram suficientes para garantir o seu crescimento. As reservas de amido nas folhas dependeram da área foliar disponível por fruto e reduziram à medida que os frutos apresentaram aumentos no diâmetro e nas massas fresca e seca.The relationship between the foliar area and the fruit growth is an important theme because affects the tree productivity and fruits quality. In this work was evaluated the effect of the foliar area on the growth of the Salustiana's sweet orange fruits. Girdled shoots of 12 months were used with a single terminal fruit and several leaves-fruit ratios. It was evaluated, weekly, the fruit growth and the leaves starch contents during 42 days. The fruit growth, evaluated in the stage II, depended on the available leaf area per fruit, provided that 30 leaves were enough to guarantee its growth. The starch reserves in the leaves depended on the available leaf area per fruit and they reduced with the increase in the diameter and dry and fresh weight of the fruits.

  1. Grazing ecology of female lambs on Italian ryegrass plus red clover pasture under different defoliation intensities Ecologia do pastejo por cordeiras em pastagem de azevém e trevo-vermelho sob diversas intensidades de desfolha

    Directory of Open Access Journals (Sweden)

    Carine Lisete Glienke

    2010-01-01

    Full Text Available The relationship between pasture dynamics and ingestive behavior of female lambs was studied on Italian ryegrass (Lolium multiflorum Lam. plus red clover (Trifolium pratense L. mixture under a range of defoliation intensities. Rotational grazing was used and the grazing interval was determined by the thermal sum of 313 degree days. The initial pre-grazing canopy height disappearance values were 65 (very high, 58 (high, 47 (medium and 37% (low. The sward vertical structure was similar among defoliation intensities. The forage allowance decreased linearly as defoliation intensities increased, with 0.35 bite/minute reduction for each 1% increase in forage allowance. The bite rate and number of bites/feeding station decreased with reduced contribution of leaves in the sward structure. It was associated, respectively, with an increase and a decrease of NDF and CP levels in forage as grazed by female lambs. The pasture cycle proves to be more important than defoliation intensities as a source for changes in feeding stations and displacement patterns of female lambs.Estudou-se a relação entre a dinâmica do pasto e o comportamento ingestivo de cordeiras em pastagem de azevém (Lolium multiflorum Lam. e trevo-vermelho (Trifolium pratense L. em diferentes intensidades de desfolha. O pastejo foi rotacionado e o intervalo entre pastejos foi determinado pela soma térmica de 313 graus-dia. Os valores de desaparecimento da altura do dossel no pré-pastejo foram de 65 (muito alta, 58 (alta, 47 (média e 37% (baixa, respectivamente. A estrutura vertical do pasto foi semelhante entre as intensidades de desfolha testadas. A oferta de forragem diminuiu linearmente com o aumento da intensidade de desfolha, com redução de 0,35 bocado/minuto a cada 1% a mais na oferta de forragem. A taxa de bocados e o número de bocados/estação alimentar reduziram com a diminuição da contribuição de folhas na estrutura da pastagem, acompanhada do aumento do teor de FDN e

  2. Capacidade de parasitismo de Trichogramma exiguum Pinto & Platner, 1978 (Hymenoptera: Trichogrammatidae criado em dois hospedeiros por diversas gerações Parasitism capacity of Trichogramma exiguum Pinto & Platner, 1978 (Hymenoptera: Trichogrammatidae reared in two hosts for several generations

    Directory of Open Access Journals (Sweden)

    Harley Nonato de Oliveira

    2005-04-01

    Full Text Available O Trichogramma é um inimigo natural dos mais estudados e utilizados atualmente no mundo. A qualidade e o desempenho de Trichogramma podem ser influenciados por alguns fatores, tal como o hospedeiro alternativo utilizado na criação massal. Objetivou-se com este trabalho foi obter informações básicas sobre os aspectos biológicos de Trichogramma exiguum Pinto & Platner, 1983 (Hymenoptera: Trichogrammatidae, criado nos hospedeiros alternativos Anagasta kuehniella (Zeller, 1879 (Lepidoptera: Pyralidae e Sitotroga cerealella (Oliv., 1819 (Lepidoptera: Gelechiidae por 10, 20 e 30 gerações, e os possíveis efeitos nas características biológicas desse parasitóide. Em cada uma das gerações avaliadas, foram utilizadas 25 fêmeas de T. exiguum recém-emergidas de cada hospedeiro, as quais foram individualizadas em tubos de vidro que continham gotículas de mel de abelha. Cada fêmea recebeu uma cartela contendo 40 ovos do hospedeiro na qual estava sendo mantido. Foi permitido um parasitismo de 24 horas para essas cartelas, sendo as mesmas trocadas diariamente até a morte da fêmea.O número de ovos parasitados no primeiro dia, a capacidade de parasitismo e a longevidade de T. exiguum foi superior quando se utilizou ovos de A. kuehniella, ao longo das diferentes gerações, mostrando ser esse hospedeiro mais indicado para a criação massal de T. exiguum.Trichogramma is one the most studied and used natural enemies of insect pests in the world. The quality and the performance of Trichogramma can be influenced by some factors, like the kind of host used for its mass rearing. The objective of this research was to obtain some basic informations about the biological aspects of Trichogramma exiguum Pinto & Platner, 1983 (Hymenoptera: Trichogrammatidae, reared on Anagasta kuehniella (Zeller, 1879 (Lepidoptera: Pyralidae and Sitotroga cerealella (Oliv., 1819 (Lepidoptera: Gelechiidae for 10, 20 e 30 generations, and the effect of something on the

  3. Diversas obras de Heery - EE.UU.

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1980-04-01

    Full Text Available In this article, different works by Heery are explained concisely, stressing some of their characteristics, such as: — the triangular configuration of the Administration Building of the Woodruff Medical Center; — the community center aspect of the Martin Luther King, Jr. Secondary School; — the energy conservation measures in the Headquarters of the Georgia Power Company; — the construction system (GALEP used in the great International Airport of Cincinnati; — the magnificent treatment of the inside of the new installations of Houdaille Industries.

    En este artículo se exponen de manera concisa diversas obras de Heery, haciendo resaltar algunas de sus características tales como: — la configuración triangular del Edificio de Administración del Centro Médico Woodruff; — el aspecto de centro comunitario de la Escuela Secundaria Martin Luther King, Jr.; — las medidas de conservación de energía de la Sede Central de la Georgia Power Company; — el magnífico tratamiento del interior de la nueva Sede de Industrias Houdaille; — el sistema de construcción (GALEP utilizado en el gran Aeropuerto Internacional de Cincinnati.

  4. Efeito da pimenta rosa associada a diversas dosagens de antibióticos em frangos de corte Effect of Brazilian red pepper meal associated with different levels of antibiotics on broilers chickens

    Directory of Open Access Journals (Sweden)

    Fabiano Gomes Gonçalves

    2012-08-01

    Full Text Available Este trabalho objetivou avaliar o efeito da adição de níveis crescentes de antibióticos associado ao farelo de pimenta rosa (FPR sobre a função hepática e desempenho de frangos de corte. Utilizou-se 528 pintos, machos, da linhagem Cobb de um dia de idade distribuídos num delineamento inteiramente casualizado com seis tratamentos, quatro repetições com 22 aves cada: T1: Controle Negativo (CN: Dieta Basal; T2: Controle Positivo (CP: Dieta Basal com 1,2% FPR; T3: CP+11ppm bacitracina Zn e 17ppm salinomicina; T4: CP+22ppm bacitracina Zn e 34ppm salinomicina; T5: CP+33ppm bacitracina Zn e 51ppm salinomicina; T6: CP + 45ppm bacitracina Zn e 67ppm salinomicina. Por conter taninos, o uso de pimenta rosa na alimentação animal requer avaliação da função hepática, sendo a dosagem das enzimas ALT (alanina aminotransferase, AST (aspartato aminotransferase e GGT (gama glutamiltransferase ferramenta auxiliar no diagnóstico de lesões hepáticas. Portanto, aos 21 e 41 dias de idade, 10 animais por tratamento foram escolhidos aleatoriamente e coletadas amostras de sangue. Aos 21 dias de idade, verificou-se efeito significativo (P0,05 do CN aos 21 e 41 dias de idade. Não houve efeito (P>0,05 dos tratamentos sobre o peso das aves e do peso relativo do fígado aos 43 dias. A adição do farelo de pimenta rosa com ou sem promotor de produção melhorou a conversão alimentar aos 43 dias de idade. Concluiu-se que a adição de 1,2% FPR não comprometeu a função hepática e que a inclusão de doses mais altas de antibióticos, associado ao FPR na dieta, resultou em alteração das enzimas hepáticas.The aim of this research was to evaluate the effects of the increase levels of antibiotics associated with the Brazilian red pepper meal (BRPM on liver function and performance of broilers. A total of 528 day-old male chicks, Cobb, distributed in a randomized design of six treatments and four replicates of 22 birds each: T1: Negative Control (NC: basal

  5. Genome sequence of Pantoea agglomerans strain IG1.

    Science.gov (United States)

    Matsuzawa, Tomohiko; Mori, Kazuki; Kadowaki, Takeshi; Shimada, Misato; Tashiro, Kosuke; Kuhara, Satoru; Inagawa, Hiroyuki; Soma, Gen-ichiro; Takegawa, Kaoru

    2012-03-01

    Pantoea agglomerans is a gram-negative bacterium that grows symbiotically with various plants. Here we report the 4.8-Mb genome sequence of P. agglomerans strain IG1. The lipopolysaccharides derived from P. agglomerans IG1 have been shown to be effective in the prevention of various diseases, such as bacterial or viral infection, lifestyle-related diseases. This genome sequence represents a substantial step toward the elucidation of pathways for production of lipopolysaccharides.

  6. Phenotypical properties of Enterobacter agglomerans (Pantoea agglomerans) from human, animal and plant sources.

    Science.gov (United States)

    Lindh, E; Kjaeldgaard, P; Frederiksen, W; Ursing, J

    1991-04-01

    Clinical, animal and plant isolates, representing different geographical areas, were identified as Enterobacter agglomerans (Pantoea agglomerans) using a quantitative bacterial dot method for DNA-DNA hybridization. The phenotypical properties of the 65 strains were investigated by conventional test methods. No strain decarboxylated ornithine. Twenty-two strains, mainly plant isolates, showed delayed acid production from alpha-methyl-glycoside, a trait which may have ecological significance. With regard to these two properties, our results differed from the description of Pantoea agglomerans given by Gavini et al. (6); further investigations will clarify these differences. Three non-pigmented, maltose-negative and salicin-negative variants were derived from yellow pigmented, maltose-positive, salicin-positive strains.

  7. Freqüência de aconselhamento para prevenção de câncer da pele entre as diversas especialidades médicas em Caxias do Sul Frequency of counseling for skin cancer prevention by the various specialties in Caxias do Sul

    Directory of Open Access Journals (Sweden)

    Adelar Bocchese Nora

    2004-02-01

    Full Text Available FUNDAMENTOS: O câncer da pele é a forma mais comum de câncer atualmente, apesar de ser um dos mais preveníveis. Não foram encontrados dados na literatura nacional quanto à freqüência de orientação para prevenção desse tipo de câncer de acordo com as diversas especialidades médicas. OBJETIVOS: O objetivo do trabalho foi verificar a freqüência de aconselhamento para prevenção de câncer da pele entre as diversas especialidades médicas em uma amostra da população de Caxias do Sul. MÉTODOS: Trata-se de um estudo transversal. Foram entrevistadas e examinadas 499 pessoas que procuraram atendimento na área de dermatologia em ações comunitárias realizadas em Caxias do Sul de janeiro a julho de 2002. RESULTADOS: Apenas 31,9% (n=159; IC 27,8 - 36,2 das pessoas entrevistadas já haviam recebido aconselhamento pela classe médica para prevenção de câncer da pele. Os pacientes de alto risco com o maior potencial para intervenção, ou seja, pacientes com idade inferior a 20 anos, receberam orientação em freqüência menor do que os pacientes com 20 anos ou mais (26,5% versus 42,5%, respectivamente; p = 0,03. A especialidade de dermatologia foi responsável por mais da metade dos aconselhamentos para prevenção de câncer da pele na população estudada. CONCLUSÃO: A freqüência de aconselhamento para prevenção de câncer da pele pelos profissionais da saúde é baixa, mesmo para os pacientes de alto risco. A orientação também varia de acordo com a especialidade consultada, tendo apenas a especialidade de dermatologia apresentado alta freqüência de aconselhamento.BACKGROUND: Nowadays, skin cancer is the most common form of cancer, in spite of being one of the most preventable forms of cancer. We did not find national data about the frequency of counseling for skin cancer prevention according to the various specialties. OBJECTIVE: The objective of this study was to verify the frequency of counseling for skin cancer

  8. The combination Enterobacter agglomerans is to be cited as Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972 and the combination Pantoea agglomerans is to be cited as Pantoea agglomerans (Beijerinck 1888) Gavini et al. 1989. Opinion 90. Judicial Commission of the International Committee on Systematics of Prokaryotes.

    Science.gov (United States)

    Tindall, B J

    2014-10-01

    The Judicial Commission affirms that, according to information presented to it, the combination Enterobacter agglomerans is to be cited as Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972 and the combination Pantoea agglomerans is to be cited as Pantoea agglomerans (Beijerinck 1888) Gavini et al. 1989. IUMS.

  9. O valor da ressonância magnética na detecção de nódulos hepáticos utilizando-se diversas técnicas ponderadas em T2: existe consenso? The value of magnetic resonance imaging for the detection of focal liver lesions using different T-2 weighted techniques: is there a consensus?

    Directory of Open Access Journals (Sweden)

    Glaucia Andrade e Silva Palácio

    2002-11-01

    Full Text Available Desde o início da década de 80 a ressonância magnética vem sendo utilizada para o estudo do abdome e principalmente na detecção de nódulos hepáticos. As imagens ponderadas em T2 são as que trouxeram maior benefício quando comparadas à tomografia computadorizada com contraste. Inúmeras técnicas e seqüências de ressonância magnética ponderadas em T2 surgiram desde então, na tentativa de aumentar a eficácia diagnóstica, com menores tempos de exame. Neste sentido, foram publicados inúmeros trabalhos demonstrando a utilidade de seqüências rápidas e ultra-rápidas, com e sem supressão de gordura, em apnéia, com sincronizador respiratório e com bobinas de sinergia, entre outros avanços tecnológicos. No entanto, não há um consenso sobre qual a técnica mais apropriada e sensível para a detecção de lesões hepáticas focais. Neste artigo fazemos uma revisão bibliográfica e análise crítica das diversas técnicas de imagens ponderadas em T2, no que diz respeito às suas sensibilidades na detecção de nódulos hepáticos.Since the early 1980's several magnetic resonance imaging pulse sequences have been developed in order to determine the optimum imaging technique for the detection and characterization of hepatic lesions. T2-weighted images play an important role in the evaluation of the liver and present equal or greater sensitivity than enhanced computed tomography for the detection of liver lesions. New techniques for obtaining T-2 weighted images have been developed in the attempt to optimize the method. These techniques have improved the image quality by shortening examination time, reducing motion artifacts, and improving contrast-to-noise ratio. The effectiveness of the different techniques (fat suppression, breath-hold, respiratory-triggered and phased-array coils has been tested in many comparative studies, although the results are controversial. In this article we review the literature and discuss the several

  10. Concentração de ácido ascórbico em sucos de diversas frutas brasileiras e sua relação com preço e necessidades diárias recomendadas de vitamina C Concentration of ascorbic acid in juices of several Brazilian fruit and its relationship with cost and daily allowances of vitamin C

    Directory of Open Access Journals (Sweden)

    Maria José Roncada

    1977-03-01

    Full Text Available Foram analisados sucos industrializados de diversas frutas brasileiras, assim como frutas naturais das espécies utilizadas na fabricação dos sucos processados, adquiridos na Capital do Estado de São Paulo, para se determinar sua concentração em ácido ascórbico. O método utilizado na dosagem do ácido ascórbico foi o de Tillmans. Os resultados foram expressos em valores médios. Dentre os sucos industrializados analisados, os sucos integrais de caju e os concentrados de laranja e tangerina, foram os que apresentaram maior teor de ácido ascórbico. Os valores médios para sucos de frutas frescas foram mais elevados que para os industrializados (exceção dos concentrados . Calculou-se também a quantidade de ácido ascórbico (em mg contida em um copo (250 ml de refresco ou de suco natural, bem como seu custo. Esses dados foram também relacionados com as necessidades diárias recomendadas, as quais poderão ser preenchidas de maneira menos dispendiosa pelas diluições necessárias de suco de caju processado ou por suco fresco de laranja.Ascorbic acid content was studied in several commercial fruit juices and in the juice of Brazilian fresh fruit of the same species used in commercial preparations, excepting those unavailable on the market at the time of the study. Tillmans' method was employed in the assessment of ascorbic acid. Results were presented in average figures. Among processed juices, whole cashew juice and concentrated orange and tangerine juices presented the highest concentration of ascorbic acid. The mean values for fresh fruit juice were higher than processed juices (excepting the concentrated ones. The quantity of ascorbic acid contained in a glass (mg per 250 ml of each of the juices was calculated as well as the cost of a glass of juice. These data were also related to daily allowances of ascorbic acid and respective cost. Daily allowances may be met more cheaply using processed cashew and fresh orange juices than

  11. O prefixo e suas diversas abordagens

    Directory of Open Access Journals (Sweden)

    Paulo Mosânio Teixeira Duarte

    2001-02-01

    Full Text Available

    Este artigo pretende apresentar criticamente as diferentes abordagens referentes aos prefixos, nas gramáticas tradicionais de língua portuguesa e em obras fundamentalistas na lingüística. Nestas, baseamo-nos nos seguintes parâmetros: transformação, produtividade, correspondência entre prefixo e preposição, perspectiva operacional. Após apresentar estes diferentes pontos de vista, concluímos não serem eles suficientes por si sós para identificar o prefixo. É conveniente reconhecer a existência de prefixos típicos, tais como in-, re- e des-, e daqueles não típicos, como sobre- e contra-, caracterizados por correspondência formal, mas não distribucional com formas livres e/ou por uso braquissêmico.

  12. Genotypic comparison of Pantoea agglomerans plant and clinical strains

    Directory of Open Access Journals (Sweden)

    Frey Jürg E

    2009-09-01

    Full Text Available Abstract Background Pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. However, commercial registration of P. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (BL2 organism due to clinical reports as an opportunistic human pathogen. This study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic markers using multi-locus phylogenetic analysis and fluorescent amplified fragment length polymorphisms (fAFLP fingerprinting. Results Majority of the clinical isolates from culture collections were found to be improperly designated as P. agglomerans after sequence analysis. The frequent taxonomic rearrangements underwent by the Enterobacter agglomerans/Erwinia herbicola complex may be a major problem in assessing clinical associations within P. agglomerans. In the P. agglomerans sensu stricto (in the stricter sense group, there was no discrete clustering of clinical/biocontrol strains and no marker was identified that was uniquely associated to clinical strains. A putative biocontrol-specific fAFLP marker was identified only in biocontrol strains. The partial ORF located in this band corresponded to an ABC transporter that was found in all P. agglomerans strains. Conclusion Taxonomic mischaracterization was identified as a major problem with P. agglomerans, and current techniques removed a majority of clinical strains from this species. Although clear discrimination between P. agglomerans plant and clinical strains was not obtained with phylogenetic analysis, a single marker characteristic of biocontrol strains was identified which may be of use in strain biosafety determinations. In addition, the lack of Koch's postulate fulfilment, rare retention of clinical strains for subsequent confirmation, and the polymicrobial nature of P

  13. Genotypic comparison of Pantoea agglomerans plant and clinical strains.

    Science.gov (United States)

    Rezzonico, Fabio; Smits, Theo H M; Montesinos, Emilio; Frey, Jürg E; Duffy, Brion

    2009-09-22

    Pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. However, commercial registration of P. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (BL2) organism due to clinical reports as an opportunistic human pathogen. This study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic markers using multi-locus phylogenetic analysis and fluorescent amplified fragment length polymorphisms (fAFLP) fingerprinting. Majority of the clinical isolates from culture collections were found to be improperly designated as P. agglomerans after sequence analysis. The frequent taxonomic rearrangements underwent by the Enterobacter agglomerans/Erwinia herbicola complex may be a major problem in assessing clinical associations within P. agglomerans. In the P. agglomerans sensu stricto (in the stricter sense) group, there was no discrete clustering of clinical/biocontrol strains and no marker was identified that was uniquely associated to clinical strains. A putative biocontrol-specific fAFLP marker was identified only in biocontrol strains. The partial ORF located in this band corresponded to an ABC transporter that was found in all P. agglomerans strains. Taxonomic mischaracterization was identified as a major problem with P. agglomerans, and current techniques removed a majority of clinical strains from this species. Although clear discrimination between P. agglomerans plant and clinical strains was not obtained with phylogenetic analysis, a single marker characteristic of biocontrol strains was identified which may be of use in strain biosafety determinations. In addition, the lack of Koch's postulate fulfilment, rare retention of clinical strains for subsequent confirmation, and the polymicrobial nature of P. agglomerans clinical reports should be considered in

  14. Clinical and microbiological characteristics of Pantoea agglomerans infection in children.

    Science.gov (United States)

    Büyükcam, Ayşe; Tuncer, Özlem; Gür, Deniz; Sancak, Banu; Ceyhan, Mehmet; Cengiz, Ali Bülent; Kara, Ateş

    2017-08-02

    Pantoea agglomerans is an environmental Gram-negative bacterium that rarely is responsible for the infections in humans but it is often a causative factor of a number of occupational diseases. This study evaluated the clinical and microbiological characteristics and pathogenicity of P. agglomerans in children. We retrospectively reviewed microbiological test results for all children (1 month old to 18 years old) who were admitted to our pediatric hospital between January 2000 to June 2015 and had positive clinical specimen cultures for P. agglomerans. Isolates were identified using conventional tests and the BBL Crystal E/NF ID or MALDI-TOF MS systems. Antibiotic susceptibilities were evaluated using the Kirby-Bauer disc diffusion method. We identified fifteen positive cultures from 14 patients with confirmed infections. The positive specimens included pus, urine, tracheal aspirate, blood, and central venous line samples that yielded P. agglomerans. The median patient age was 8.8 years (range: 1.5 months to 16.5 years), and all patients had underlying comorbidities. Five patients had medical devices, and two devices were removed. The most common P. agglomerans infections involved wound infections (35.7%), pneumonia (21.4%), and urinary tract infections (21.4%). Three patients had concomitant infections (Enterococcus faecium, Pseudomonas aeruginosa, and Aspergillus fumigatus). Five patients had anemia. Three patients (21.4%) died, and all three had carbapenem-resistant P. agglomerans that was detected after the first week of hospitalization; two cases involved pneumonia, which was ineffectively treated. P. agglomerans infections may be life-threatening, especially in young patients with pneumonia. Hospital-acquired P. agglomerans may have different pathogenicity and clinical features, compared to community-acquired P. agglomerans, although further studies are needed to understand the drug-resistance patterns in this bacterium. Copyright © 2017 The Authors

  15. Enterobacter agglomerans: the clinically important plant pathogen.

    Science.gov (United States)

    Geere, I. W.

    1977-01-01

    During a 5-month period Enterobacter agglomerans, now described as a member of the phytopathogenic genus Erwinia, was isolated from 13 patients in a general hospital; in 1 patient it was isolated from two sites. In six instances the organism was the sole pathogen isolated, in two instances it may have contributed to infection and in the remaining instances it was probably a transient saprophyte. The strains showed some variation in biochemical reactions but were similar in colonial morphology and were consistently sensitive to several antibiotics. Although this organism is prevalent in the general environment and usually relatively benign, it does have a potential for nosocomial infection. PMID:837319

  16. Interaction microenvironment - Pantoea agglomerans limits maize yield

    Directory of Open Access Journals (Sweden)

    Daniel Ruíz Juárez

    2018-01-01

    Full Text Available Pantoea agglomerans has been reported as the cause of chlorotic streaks on maize leaves in the Central High Valleys of México (CVHM, but there are no current data of how the infection of this new pathogen in Mexico affects production and yield of the crop. To understand the development of the disease in the crop, two experiments with a split-plot design and three replications were established during spring-summer 2010 in different microenvironments: Ayapango and Temamatla. Three cultivars of maize (a tri-linear HS2 hybrid, the single-cross hybrid Triunfo and the native Cacahuacintle were evaluated with three P. agglomerans isolates. In the three maize cultivars, chlorotic streaks appeared on new leaves; at the ripening stage, the symptoms were less perceptible. The average incidence of plants with chlorotic streaks was higher in the town of Temamatla than in Ayapango, and in both microenvironments, the average degree of severity was less than 40%. Isolate A was the most virulent (P < 0.0001 on the three cultivars evaluated. These results will provide the basis for effective management of the disease under environmental conditions similar to those evaluated in this research.

  17. Pantoea agglomerans endophthalmitis: clinical features and outcomes.

    Science.gov (United States)

    Sudhalkar, Aditya; Majji, Ajit B; Chhablani, Jay; Manderwad, Guruprasad

    2014-08-01

    To determine the clinical profile and outcomes of patients with Pantoea agglomerans endophthalmitis as seen at a tertiary eye care center in India. Retrospective observational case series. Data collected included demographics, history, the initial and final corrected distance visual acuity, details of the ocular and systemic examination, surgeries performed, and the final anatomical outcome. The final corrected distance visual acuity and the anatomical outcome were the outcome measures. Four patients had traumatic endophthalmitis; the fifth developed endophthalmitis after cataract surgery. All patients were men with a mean age of 34.24 ± 23.34 years. Three patients had corneal laceration and traumatic cataract. All patients underwent vitreous sampling with intraocular antibiotic injection with or without lensectomy and corneal wound repair. The visual acuity at presentation ranged from hand motion to perception of light. One patient ended up with phthisis in the affected eye. One patient developed postoperative retinal detachment and underwent successful surgery. The final visual acuity ranged from 20/25 to no light perception. P. agglomerans is a likely source of infection in traumatic and postoperative cases. Though variable, it appears sensitive to common antibiotics. The visual and anatomical prognosis seems fair in most cases. Special care needs to be taken to ensure the organism is not missed, given the rarity of the condition and the isolation techniques required.

  18. Pantoea agglomerans Infections in Children: Report of Two Cases

    OpenAIRE

    Siwakoti, Shraddha; Sah, Rinku; Rajbhandari, Rupa Singh; Khanal, Basudha

    2018-01-01

    Introduction. Pantoea agglomerans, primarily an environmental and agricultural organism has been reported as both commensal and pathogen of humans. We present two case reports of P. agglomerans infections in children that involved the meninges and bloodstream. Case Presentations. A 6-month-old female baby, diagnosed as congenital hydrocephalus secondary to aqueduct stenosis with ventriculoperitoneal shunt in situ, operated 14 days back was brought to the pediatric emergency with a two-day his...

  19. Pantoea agglomerans, a Plant Pathogen Causing Human Disease▿

    Science.gov (United States)

    Cruz, Andrea T.; Cazacu, Andreea C.; Allen, Coburn H.

    2007-01-01

    We present 53 pediatric cases of Pantoea agglomerans infections cultured from normally sterile sites in patients seen at a children's hospital over 6 years. Isolates included 23 from the bloodstream, 14 from abscesses, 10 from joints/bones, 4 from the urinary tract, and 1 each from the peritoneum and the thorax. P. agglomerans was most associated with penetrating trauma by vegetative material and catheter-related bacteremia. PMID:17442803

  20. Contact lens-related polymicrobial keratitis from Pantoea agglomerans and Escherichia vulneris

    Directory of Open Access Journals (Sweden)

    Vincent D. Venincasa

    2016-04-01

    Conclusions: This is the first report of P. agglomerans and E. vulneris keratitis in association with contact lens wear. Both strains of P. agglomerans and E. vulneris were pansensitive to all tested antibiotics.

  1. Pantoea agglomerans Infections in Children: Report of Two Cases

    Directory of Open Access Journals (Sweden)

    Shraddha Siwakoti

    2018-01-01

    Full Text Available Introduction. Pantoea agglomerans, primarily an environmental and agricultural organism has been reported as both commensal and pathogen of humans. We present two case reports of P. agglomerans infections in children that involved the meninges and bloodstream. Case Presentations. A 6-month-old female baby, diagnosed as congenital hydrocephalus secondary to aqueduct stenosis with ventriculoperitoneal shunt in situ, operated 14 days back was brought to the pediatric emergency with a two-day history of high fever associated with vomiting, irritability, excessive crying, and decreased feeding. Postoperative meningitis was confirmed as cerebrospinal fluid culture revealed P. agglomerans. She responded well with a 14-day intravenous (IV course of ceftriaxone. Also, we report a case of a 3-year-old male child referred to our center with a provisional diagnosis of UTI with chickenpox for further evaluation. During his 24-hour stay at the local hospital, he had received oral antibiotics and urinary catherization. Urine culture of catheter clamp urine was sterile. P. agglomerans was grown in blood culture. He was treated successfully with IV ceftriaxone and amikacin. Conclusion. P. agglomerans can cause postsurgical meningitis and bloodstream infection in children. The clinical course of infection was mild and timely administration of proper antibiotic resulted in a favorable outcome.

  2. Pantoea agglomerans Infections in Children: Report of Two Cases.

    Science.gov (United States)

    Siwakoti, Shraddha; Sah, Rinku; Rajbhandari, Rupa Singh; Khanal, Basudha

    2018-01-01

    Pantoea agglomerans, primarily an environmental and agricultural organism has been reported as both commensal and pathogen of humans. We present two case reports of P. agglomerans infections in children that involved the meninges and bloodstream. A 6-month-old female baby, diagnosed as congenital hydrocephalus secondary to aqueduct stenosis with ventriculoperitoneal shunt in situ, operated 14 days back was brought to the pediatric emergency with a two-day history of high fever associated with vomiting, irritability, excessive crying, and decreased feeding. Postoperative meningitis was confirmed as cerebrospinal fluid culture revealed P. agglomerans . She responded well with a 14-day intravenous (IV) course of ceftriaxone. Also, we report a case of a 3-year-old male child referred to our center with a provisional diagnosis of UTI with chickenpox for further evaluation. During his 24-hour stay at the local hospital, he had received oral antibiotics and urinary catherization. Urine culture of catheter clamp urine was sterile. P. agglomerans was grown in blood culture. He was treated successfully with IV ceftriaxone and amikacin. P. agglomerans can cause postsurgical meningitis and bloodstream infection in children. The clinical course of infection was mild and timely administration of proper antibiotic resulted in a favorable outcome.

  3. Comportamento ingestivo diurno de vacas primíparas em pastagem nativa dominada por capim-annoni-2 com suplementação proteica e mineral em diversas estações climáticas Diurnal ingestive behaviour of pregnant heifers grazing on natural grasslands invaded by Eragrostis plana Ness as affected by protein and mineral supplements in the different climatic seasons

    Directory of Open Access Journals (Sweden)

    Silvane Barcelos Carlotto

    2010-03-01

    Full Text Available Avaliou-se a influência da suplementação proteica e mineral sobre o comportamento ingestivo de vacas primíparas em pastagem nativa dominada por capim-annoni-2 (Eragrostis plana Ness recebendo suplementação com sal comum; sal mineral; sal proteinado; ou sal para reprodução e sal proteinado (1:1. Testou-se a hipótese de que suplementos minerais e proteinados pudessem promover alterações no comportamento ingestivo dos animais em pastejo. O estudo foi desenvolvido em uma área de 37 ha de pastagem nativa invadida por capim-annoni-2, dividida em oito potreiros (unidades experimentais. Os animais foram avaliados no período diurno, por dois dias consecutivos, em cada uma das estações climáticas, de abril de 2006 a março de 2007. O delineamento experimental foi inteiramente casualizado com duas repetições. Os tempos de pastejo, de ruminação, de ócio e de outras atividades não diferiram entre suplementos, e os valores médios diários para essas atividades foram 505, 108, 70 e 11 minutos, respectivamente. Os tempos de pastejo, ruminação e ócio e a taxa de bocados diferiram significativamente entre as estações climáticas. A suplementação proteica e mineral não promove alterações significativas no comportamento ingestivo dos animais. O comportamento ingestivo, no entanto, é influenciado pelas estações climáticas.The influence was assessed of protein and mineral supplementation on the ingestive behavior of pregnant heifers on a native grassland dominated by capim-annoni-2 (Eragrostis plana Ness supplementation with common salt, mineral salt, protein salt and protein salt and reproduction salt (1:1. The hypothesis was tested that different mineral and protein salt supplements could promote alterations in the animal grazing ingestive behavior. The study was carried out in a 37 ha area of native pasture invaded by capim-annoni-2, divided into 8 paddocks (experimental units. The animals were evaluated during the daylight

  4. Biotransformation, by Enterobacter agglomerans, of pyrimidine acyclonucleosides to acyclonucleotides.

    Science.gov (United States)

    Rutkowski, M; Korczak, E

    1992-06-15

    The ability of Enterobacter agglomerans to transform naturally occurring nucleosides into nucleotides has been utilized to transform newly synthesized pyrimidine acyclonucleosides into the corresponding acyclonucleotides. Unselected bacteria were used as the source of nucleoside phosphotransferase, the phosphate group donor being 4-nitrophenyl phosphate in the presence of Zn2+ ions. Optimal reaction conditions are outlined.

  5. Pantoea agglomerans foreign body-induced septic arthritis.

    Science.gov (United States)

    Rave, Omer; Assous, Marc Victor; Hashkes, Philip J; Lebel, Ehud; Hadas-Halpern, Irit; Megged, Orli

    2012-12-01

    A 4-year-old boy was admitted because of left knee arthritis. Synovial fluid culture yielded Pantoea agglomerans identified by 16S rDNA polymerase chain reaction. Ultrasound examination revealed a foreign body in the synovial fluid. The patient underwent arthroscopy with removal of a thorn. This article highlights the need to search for a foreign body in Pantoea septic arthritis.

  6. Estratégia de comercialização em mercados derivativos - descobrimento de base e risco de base da cafeicultura em diversas localidades de Minas Gerais e São Paulo Marketing strategy in derivative markets discovery of the basis and the risk of basis of coffee in several places in Minas Gerais and São Paulo states

    Directory of Open Access Journals (Sweden)

    Renato Elias Fontes

    2005-04-01

    Full Text Available Com o presente trabalho tem-se por objetivo geral mensurar os valores da base e do risco de base da atividade cafeeira de importantes cidades produtoras de café localizadas no Estados de Minas Gerais e São Paulo, pois a estratégia de se utilizar os mercados derivativos vem ganhando cada vez mais importância, devido às suas características, que propiciam o feitio de ''hedge'' de preços. Os municípios estudados foram Boa Esperança, Caratinga, Lavras, Machado, Patrocínio, Três Pontas, Varginha e Garça (SP. Os dados primários utilizados sobre o preço da saca de café comercializada no mercado local foram coletados diretamente nas instituições participantes, e o preço do café no mercado futuro foi levantando junto à BM&F. A diversidade dos valores da base e do risco de base encontrada demonstra que cada localidade apresenta características próprias de comercialização, e todas as localidades apresentam valores negativos. A época de comercialização é um importante fator a ser levado em consideração, pois na safra há um fortalecimento da base e o risco de base é menor do que na entressafra, que apresenta um enfraquecimento da base.The general objective of this assignment is to measure the value of the basis and of the risk of basis of the cultivation of coffee in important coffee producer cities from the states of Minas Gerais and São Paulo, for the strategy of using the derivative markets has been obtaining more importance due to its characteristics that provide the hedge of the prices. The cities studied were Boa Esperança, Caratinga, Lavras, Machado, Patrocínio, Três Pontas, Varginha and Garça (SP. The primary data used was, the local price of the coffee bag. The data was collected directly from the participant institutions and the future price of the coffe was raised with the BM&F. The variety of the values of the basis and of the risk of basis found shows that each locality presents its own commerce

  7. Evaluation of three differential media for detection of Enterobacter agglomerans (Erwinia herbicola).

    Science.gov (United States)

    Bucher, C; von Graevenitz, A

    1982-01-01

    Dextrin-fuchsine-sulfite medium (DFS), Rimler-Shotts agar (RS), and a new lysine-ornithine-mannitol agar (LOM) were tested for detection of Enterobacter agglomerans. In human stools, LOM and DFS were most sensitive at coliform-to-E. agglomerans ratios of less than or equal to 10(2) and E. agglomerans inocula of greater than 10(2) per plate. Both LOM and DFS detected one strain in 254 stools, but RS proved to be inhibitory. PMID:6809787

  8. Evaluation of three differential media for detection of Enterobacter agglomerans (Erwinia herbicola).

    OpenAIRE

    Bucher, C; von Graevenitz, A

    1982-01-01

    Dextrin-fuchsine-sulfite medium (DFS), Rimler-Shotts agar (RS), and a new lysine-ornithine-mannitol agar (LOM) were tested for detection of Enterobacter agglomerans. In human stools, LOM and DFS were most sensitive at coliform-to-E. agglomerans ratios of less than or equal to 10(2) and E. agglomerans inocula of greater than 10(2) per plate. Both LOM and DFS detected one strain in 254 stools, but RS proved to be inhibitory.

  9. [Pantoea agglomerans: a new pathogen at the neonatal intensive care unit?].

    Science.gov (United States)

    Segado-Arenas, Antonio; Alonso-Ojembarrena, Almudena; Lubián-López, Simón P; García-Tapia, Ana M

    2012-08-01

    Late-onset sepsis is very frequent among preterm infants and cases due to Gram negative pathogens have elevated morbidity and mortality. Pantoea agglomerans is a Gram negative organism which has been rarely reported causing disease in humans. We present a case of P. agglomerans late-onset fulminant sepsis in a preterm newborn at a neonatal intensive care unit. Up to date none P. agglomerans sepsis has been reported among this population in our country.

  10. Six cases of sepsis caused by Pantoea agglomerans in a teaching hospital.

    Science.gov (United States)

    Liberto, Maria Carla; Matera, Giovanni; Puccio, Rossana; Lo Russo, Teresa; Colosimo, Elena; Focà, Emanuele

    2009-01-01

    Pantoea agglomerans is an environmental organism which may seldom cause opportunistic infections. Here we report on a 6 case outbreak in a teaching hospital. Within three months . agglomerans was isolated from blood cultures of 5 patients from oncology and 1 patient from ICU departments. P. agglomerans was in pure culture in 5 cases, while in the last one Rahnella aquatilis and Candida famata were also isolated. Therefore, P. agglomerans is able to produce nosocomial infections in patients with primary pathology often associated with immune suppression.

  11. MODELO PARA LA INTEROPERABILIDAD DE CONTENIDOS EDUCATIVOS EN DIVERSAS PLATAFORMAS

    Directory of Open Access Journals (Sweden)

    Adriana Xiomara Reyes-Gamboa

    2014-01-01

    Full Text Available Este artículo presenta los resultados del proyecto de interoperabilidad entre plataformas “diseño de un modelo para la interoperabilidad de contenidos educativos en las diversas plataformas basadas en la nube”, el cual busca el correcto despliegue de contenidos audiovisuales en las diferentes plataformas de los dispositivos, el modelo desarrollado se basó en una caracterización de cada una de las plataformas en busca de funcionalidades comunes entre ellas. Este modelo fue validado mediante un caso práctico donde un grupo de personas utilizo el modelo y evaluó su desempeño y despliegue de los contenidos en cada uno de los dispositivos

  12. Detection of Pantoea agglomerans in hip prosthetic infection by sonication of the removed prosthesis: the first reported case.

    Science.gov (United States)

    Hischebeth, Gunnar T R; Kohlhof, Hendrik; Wimmer, Matthias D; Randau, Thomas M; Bekeredjian-Ding, Isabelle; Gravius, Sascha

    2013-01-01

    Pantoea agglomerans is a rare isolate in orthopaedic patients. We describe the first case of an acute hip prosthetic joint infection (PJI) caused by Pantoea agglomerans. The microorganism was detected after sonication of the removed hip endoprosthesis.

  13. 40 CFR 180.1267 - Pantoea agglomerans strain C9-1; exemption from the requirement of a tolerance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Pantoea agglomerans strain C9-1... RESIDUES IN FOOD Exemptions From Tolerances § 180.1267 Pantoea agglomerans strain C9-1; exemption from the... Pantoea agglomerans strain C9-1 when used on apples and pears. [71 FR 24596, Apr. 26, 2006] ...

  14. 40 CFR 180.1272 - Pantoea agglomerans strain E325; exemption from the requirement of a tolerance.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Pantoea agglomerans strain E325... RESIDUES IN FOOD Exemptions From Tolerances § 180.1272 Pantoea agglomerans strain E325; exemption from the... Pantoea agglomerans strain E325 when used on apples and pears. [71 FR 54933, Sept. 20, 2006] ...

  15. Salt stress sensitivity of nitrogen fixation in Enterobacter agglomerans strains.

    Science.gov (United States)

    Rai, Raman; Rieder, Gabriele

    1998-12-01

    Two strains 333 to 339 of Enterobacter agglomerans were selected in the present study to evaluate the response of increasing concentrations of NaCl on growth, N(2)-fixation, and nitrogenase activity/synthesis. E. agglomerans strains 333 and 339 showed optimum growth and acetylene-reducing activity with 0.5 to 1.0% NaCl in a nitrogen-free minimal medium (NFDM) with glucose, respectively, in 28 h incubation, although both strains displayed better growth and acetylene-reducing activity with 3.0% and 2.0% NaCl after 52 h and 100 h incubation periods than the 28 h culture did. Our experiments with shiftings of salt concentrations in NFDM medium indicated that a synthesis of nitrogenase enzyme was generally more sensitive to higher concentrations of NaCl than nitrogenase activity was.

  16. D-tyrosine affects aggregation behavior of Pantoea agglomerans.

    Science.gov (United States)

    Yang, Jing; Yu, Jiajia; Jiang, Jing; Liang, Chen; Feng, Yongjun

    2017-02-01

    D-amino acids have been proved to disassemble biofilms by disassociating the matrix. Pantoea agglomerans is characterized by the formation of another kind of multicellular structure called symplasmata, which also remains the ability to form biofilms. In this study, a rice diazotrophic endophyte P. agglomerans YS19 was selected as a model strain to explore the effects of D-amino acids on these two kinds of cell aggregate structures. It was discovered that D-tyrosine disassociates biofilm, yet promotes symplasmata formation. D-tyrosine showed no influence on bacterial growth yet promoted the bacterial motility and inhibited the expression of cellular MalE and OmpF proteins, which enriched our knowledge of the biological effect of D-amino acids and expanded the research ideas of symplasmata formation. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Chitinolytic Enterobacter agglomerans Antagonistic to Fungal Plant Pathogens

    OpenAIRE

    Chernin, L.; Ismailov, Z.; Haran, S.; Chet, I.

    1995-01-01

    Three Enterobacter agglomerans strains which produce and excrete proteins with chitinolytic activity were found while screening soil-borne bacteria antagonistic to fungal plant pathogens. The chitinolytic activity was induced when the strains were grown in the presence of colloidal chitin as the sole carbon source. It was quantitated by using assays with chromogenic p-nitrophenyl analogs of disaccharide, trisaccharide, and tetrasaccharide derivatives of N-acetylglucosamine. A set of three flu...

  18. [SEROLOGICAL PROPERTIES AND BIOLOGICAL ACTIVITY OF PANTOEA AGGLOMERANS LIPOPOLYSACCHARIDES].

    Science.gov (United States)

    Bulygina, T V; Yakovleva, L M; Brovarska, O S; Varbanets, L D

    2015-01-01

    The serological and phytotoxic properties of lipopolysaccharide (LPS) of plant pathogens--Pantoea agglomerans were studied. It is known that the thin variations in the structure of the O-specific polysaccharides determining serological specificity of gram- negative bacteria and used as a molecular basis of serological classification schemes. For P. agglomerans still does not exist a classification scheme based on serology specificity of their LPS. The results of cross serological tests demonstrate immunochemical heterogeneity of species P agglomerans. Only three strains of the 8488, 8490 and 7969 according to the agglutination of O-antigens and direct hemagglutination and inhibition direct hemagglutination can be attributed to a single serogroup. Other strains--each separate group, although some have a relationship. Compared with control plants under the influence of seed treatment of LPS in plants may be reduced, and in some cases increased root length, height and weight sprout, depending on the strain from which the selected LPS. Dive seedlings of tomatoes in the solutions of the studied preparations FSC caused the loss, and after some time, restore turgor.

  19. Draft Genome Sequence of the Antibiotic-Producing Cystic Fibrosis Isolate Pantoea agglomerans Tx10

    OpenAIRE

    Smith, Derek D. N.; Kirzinger, Morgan W. B.; Stavrinides, John

    2013-01-01

    Pantoea agglomerans is an enteric bacterium that is capable of causing both plant and human disease. Here, we report the genome sequence of a cystic fibrosis isolate, P.?agglomerans Tx10, which produces an antibiotic that is effective against Staphylococcus aureus.

  20. Polyphasic study of plant- and clinic-associated Pantoea agglomerans strains reveals indistinguishable virulence potential.

    Science.gov (United States)

    Völksch, Beate; Thon, Susanne; Jacobsen, Ilse D; Gube, Matthias

    2009-12-01

    Pantoea species are ubiquitous in nature and occasionally associated with infections caused by contaminated clinical material. Hence, Pantoea agglomerans is considered as an opportunistic pathogen of humans. Since species of the genus Pantoea and closely related species of other Enterobacteriaceae genera are phenotypically very similar, many clinical isolates are misassigned into P. agglomerans based on the use of quick commercial-offered biochemical tests. Our objective was to find markers enabling discrimination between clinical and plant isolates and to assess their virulence potential. We characterized 27 Pantoea strains, including 8 P. agglomerans isolates of clinical, and 11 of plant origin by biochemical tests and genotyping, including analysis of 16S rDNA and gapA gene sequences, and pattern polymorphisms of ITS- and ERIC/REP-DNA. All data showed that no discrete evolution occurred between plant-associated and clinical P. agglomerans isolates. Based on the typing results, five clinical- and five plant-associated P. agglomerans strains representing the majority of clades were tested on a model plant and in embryonated eggs. On soybean plants P. agglomerans strains independent of their origin could develop stable epiphytic populations. Surprisingly, in the embryonated egg model there was no difference of virulence between clinical and vegetable P. agglomerans isolates. However, these strains were significantly less virulent than a phytopathogenic P. ananatis isolate. We suggest that, independent of their origin, all P. agglomerans strains might possess indistinguishable virulence potential.

  1. Avaliação de 166 ETEs em operação no país, compreendendo diversas tecnologias. Parte 1: análise de desempenho Evaluation of 166 treatment plants operating in Brazil, comprising several technologies. Part 1: performance analysis

    Directory of Open Access Journals (Sweden)

    Sílvia M. A. Corrêa Oliveira

    2005-12-01

    Full Text Available O trabalho apresenta uma análise de desempenho de 166 estações de tratamento de esgotos urbanos em operação nos estados de Minas Gerais e de São Paulo. Foram investigadas seis modalidades de tratamento diferentes, compreendendo os processos: (a fossa séptica seguida de filtro anaeróbio, (b lagoas facultativas, (c lagoas anaeróbias seguidas por lagoas facultativas, (d lodos ativados, (e reatores UASB operando isoladamente e (f reatores UASB seguidos por pós-tratamento. A avaliação considerou as concentrações efluentes e as eficiências de remoção dos constituintes DBO, DQO, SST, NTK, PT e coliformes (organismos termotolerantes, que foram comparadas com valores típicos reportados na literatura técnica. Em geral, o desempenho na remoção de matéria orgânica esteve dentro do esperado. As eficiências de remoção de SST estiveram abaixo, enquanto a remoção de coliformes foi maior que a descrita na literatura. A remoção de nutrientes foi baixa, já que nenhuma das modalidades analisadas foi projetada com este objetivo.The paper analyses the performance of 166 wastewater treatment plants operating in Brazil. Six different treatment technologies have been investigated, comprising the following processes: septic tank + anaerobic filter, facultative pond, anaerobic pond + facultative pond, activated sludge, UASB reactors alone, UASB reactors followed by post-treatment. The objective of the research was to evaluate the effluent quality and the removal efficiencies of the parameters BOD, COD, TSS, coliforms, TKN and phosphorus. The paper compares the effluent quality and the removal efficiencies with typical values reported by the technical literature. In general, the performance in the removal of organic matter was as expected. TSS removal efficiencies were lower than reported, but coliform removal was higher than described in the literature. Nutrient removal was low, but none of the treatment processes have been designed with

  2. Avaliação da sensibilidade de diversas espécies de plantas daninhas aquáticas ao carfentrazone-ethyl, em ambiente controlado Assessment of sensitivity of several aquatic weeds to carfentrazone-ethyl under controlled environment

    Directory of Open Access Journals (Sweden)

    L.L. Foloni

    2005-06-01

    Full Text Available Plantas aquáticas, especialmente macrófitas, tornam-se sério problema em hidrelétricas, afetando a múltipla utilização dos corpos d'água, incluindo produção de peixes e atividades de pesca, perdas d'água por evapotranspiração, esportes aquáticos, canoagem, irrigação e produção de energia nas usinas hidrelétricas. Com o objetivo de analisar o potencial de uso do carfentrazone-ethyl no controle das principais plantas daninhas aquáticas no Brasil, foi instalado um experimento em vasos com água. Utilizaram-se os seguintes tratamentos herbicidas (g i.a. ha-1: carfentrazone-ethyl a 15, 30 e 60; glyphosate a 4.536; 2,4-D a 4.690; imazapyr a 1.250; e uma testemunha sem herbicida. Esses tratamentos foram testados nas seguintes espécies: Eichhornia crassipes, Salvinia auriculata, Pistia stratiotes, Myriophyllum aquaticum, Brachiaria arrecta, Hydrocotyle umbellata, Typha sp. e Echinochloa polystachya. As avaliações foram efetuadas aos 7, 14, 21 e 28 dias após os tratamentos. Os resultados mostraram que o carfentrazone-ethyl foi eficiente no controle de E. crassipes (maior dose e P. stratiotes (duas maiores doses, com efeito supressivo sobre S. auriculata. Foi observado que nas outras plantas daninhas estudadas não houve eficiência de controle.Aquatic weeds, especially macrophytes, are a serious problem in hydroelectric plant systems, affecting the multiple use of water, such as fish production and fishing activities, water losses by evapotranspiration, aquatic sports, boating, irrigation and hydroelectric power production. An experiment using boxes filled with water was carried out to analyze the potential use of carfentrazone-ethyl in the control of the main aquatic weeds in Brazil. The following herbicide treatments (g i.a. ha-1 were used: carfentrazone-ethyl at 15, 30 and 60; glyphosate at 4,536; 2,4D at 4,690; imazapyr at 1,250, and no herbicide. These treatments were tested on the species: Eichhornia crassipes, Salvinia

  3. A Psicologia Ambiental e as diversas realidade humanas

    Directory of Open Access Journals (Sweden)

    Esther Wiesenfeld

    2005-01-01

    Full Text Available Este artigo baseia as respostas quanto ao objeto da psicologia ambiental e seus aspectos constitutivos na análise dos periódicos Environment & Behavior, Journal of Environmental Psychology, congressos e estudos latino-americanos. Considera que a definição do objeto, circunscrita a perspectivas que diferem em suas dimensões ontológicas, epistemológicas e metodológicas, ocasiona várias psicologias ambientais. Para contribuir para o desenvolvimento do conhecimento e favorecer as condições de vida e do planeta, as distintas psicologias ambientais deveriam dialogar. Conclui que o enfoque adotado é que levaria a abordar a realidade de modos distintos.

  4. Impacto na qualidade de vida em pacientes com hanseníase: correlação do Dermatology Life Quality Index com diversas variáveis relacionadas à doença Impact on the quality of life of patients with Hansen’s disease: correlation between Dermatology Life Quality Index and disease status

    Directory of Open Access Journals (Sweden)

    Bruna Dacier Lobato Martins

    2008-02-01

    Full Text Available FUNDAMENTOS: A hanseníase é doença infectocontagiosa crônica causada pelo Micobacterium leprae. Caracteriza-se por acometimento dermatoneurológico, variando em espectro entre dois pólos estáveis. Apesar de curável, ainda representa relevante problema de saúde pública, sendo o Brasil o segundo país mais endêmico do mundo. Sua maior morbidade associa-se aos estados reacionais e ao acometimento neural, podendo causar incapacidades físicas permanentes que comprometem significativamente a qualidade de vida dos pacientes, com auto-estigmatização e vergonha. OBJETIVO: Avaliar o grau de comprometimento da qualidade de vida nos pacientes com hanseníase. MÉTODOS: Estudo observacional com correlação entre variáveis clínicas de gravidade da doença e questionário de avaliação da qualidade de vida: Dermatology Life Quality Index. RESULTADOS: Dos 40 pacientes avaliados, a maioria apresentou comprometimento da qualidade de vida de grave a muito grave segundo score obtido no Dermatology Life Quality Index. As variáveis analisadas individualmente também mostraram correlação de gravidade com o número de pontos obtido. CONCLUSÃO: A hanseníase causa sofrimento que ultrapassa a dor e o mal-estar estritamente vinculados ao prejuízo físico, com grande impacto social e psicológico, justificando tanto avanços para abordagem multidisciplinar ao paciente quanto a necessidade de ações de saúde que visem ao controle da doença.BACKGROUND: Hansen’s disease is a chronic infecto-contagious illness caused by Mycobacterium leprae. It is characterized by dermato-neurological damage, varying in spectrum between two steady poles. Albeit curable, it still represents an important public health problem, Brazil being the second most endemic country in the world. Its higher morbidity is associated with reactions and neural damage, which is able to cause permanent disabilities that significantly compromising the quality of life of the patients

  5. Proposal to acknowledge Beijerinck as the original author of the species Pantoea agglomerans. Request for an opinion.

    Science.gov (United States)

    Edwards, Jane; Baque, Ellen; Tang, Jane

    2004-11-01

    The name 'Bacillus agglomerans' was first published by Beijerinck in 1888. Ewing and Fife changed the name to Enterobacter agglomerans in 1972 as a new combination, acknowledging that Beijerinck had been the original author and making the new full name Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972. Beijerinck's name was omitted from the Approved Lists of Bacterial Names in 1980, which listed only Ewing and Fife as the authors. The current listings in the List of Bacterial Names with Standing in Nomenclature follow the Approved Lists and omit Beijerinck from the names for both Enterobacter agglomerans and Pantoea agglomerans. It is proposed that Beijerinck be acknowledged as the original author of this species by having his name reinstated in its full name, Pantoea agglomerans (Beijerinck 1888) Gavini et al. 1989, and a Request for an Opinion is put forward to the Judicial Commission.

  6. Association and attraction of blueberry maggot fly Curran (Diptera: Tephritidae) to Pantoea (Enterobacter) agglomerans.

    Science.gov (United States)

    MacCollom, G B; Lauzon, C R; Sjogren, R E; Meyer, W L; Olday, F

    2009-02-01

    The attraction of washed, medium-free cells of Pantoea (Enterobacter) agglomerans to wild, adult Rhagoletis mendax Curran, the blueberry maggot fly, was evaluated in managed blueberry fields in Maine. Attraction was evaluated using Pherocon AM and Ladd traps, each tested with or without washed bacterial cells. Field studies showed significant increases in fly captures on the Pherocon AM traps. Apple volatiles odors on Ladd traps seemed to cancel the effects of bacterial odors. Aerobic heterotrophic bacteria were isolated and identified from alimentary organs within wild R. mendax. Isolates indentified included P. agglomerans. Blueberries collected in the field were surveyed for the presence of P. agglomerans and blueberries containing blueberry maggot larvae and noninfested blueberries were analyzed for amino acid content. Maggot-infested blueberry contained twice the amino acid nitrogen than that of noninfested blueberry. P. agglomerans, like with other pest tephritids, seems to be a cosmopolite with blueberry maggot.

  7. A case of bilateral endogenous Pantoea agglomerans endophthalmitis with interstitial lung disease.

    Science.gov (United States)

    Seok, Susie; Jang, Young Jun; Lee, Seung Woo; Kim, Ho Chang; Ha, Gyoung Yim

    2010-08-01

    We here in report a case of bilateral endogenous endophthalmitis caused by Pantoea agglomerans (P. agglomerans) in a patient who had interstitial lung disease and was treated with oral corticosteroids. A 72-year-old man presented with decreased visual acuity in both eyes nine days after he received oral corticosteroids. He had marked uveitis, cataracts, and vitreous opacities. Cultures were taken of blood, aqueous humor, and vitreous. We initially suspected a fungal etiology and treated him with antifungal drugs; however, the intraocular disease progressed without improvement. Vitreous culture was positive for P. agglomerans. The patient underwent pars plana vitrectomy with cataract surgery bilaterally, followed by a 2-week course of antibiotics. The final visual acuity was 20/25 in the right eye and 20/200 in the left eye. This is the first report of bilateral endogenous endophthalmitis caused by P. agglomerans in Korea; it is also the first case reported outside of the United States.

  8. Branchial challenge of normal subjects with the endotoxin of Enterobacter agglomerans isolated from cotton dust.

    Science.gov (United States)

    Jamison, J P; Lowry, R C

    1986-05-01

    Endotoxin produced by a culture of Enterobacter agglomerans isolated from cotton dust was inhaled by 12 normal subjects. No significant airway constriction was obtained in doses equivalent to those experienced in a workshift in a dusty mill. There was a statistically significant difference between this result and the bronchoconstriction that had occurred after flax dust inhalation in the same subjects. It is suggested that Enterobacter agglomerans endotoxin is not the causative agent of the acute bronchoconstriction that follows inhalation of textile dust.

  9. Branchial challenge of normal subjects with the endotoxin of Enterobacter agglomerans isolated from cotton dust.

    OpenAIRE

    Jamison, J P; Lowry, R C

    1986-01-01

    Endotoxin produced by a culture of Enterobacter agglomerans isolated from cotton dust was inhaled by 12 normal subjects. No significant airway constriction was obtained in doses equivalent to those experienced in a workshift in a dusty mill. There was a statistically significant difference between this result and the bronchoconstriction that had occurred after flax dust inhalation in the same subjects. It is suggested that Enterobacter agglomerans endotoxin is not the causative agent of the a...

  10. Genotypic comparison of Pantoea agglomerans plant and clinical strains

    OpenAIRE

    Frey Jürg E; Montesinos Emilio; Smits Theo HM; Rezzonico Fabio; Duffy Brion

    2009-01-01

    Abstract Background Pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. However, commercial registration of P. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (BL2) organism due to clinical reports as an opportunistic human pathogen. This study compares plant-origin and clinical strains in a search for discriminati...

  11. Nitrogen-fixing Enterobacter agglomerans isolated from guts of wood-eating termites.

    Science.gov (United States)

    Potrikus, C J; Breznak, J A

    1977-02-01

    Two strains of facultatively anaerobic, N2-fixing bacteria were isolated from guts of Coptotermes formosanus and identified as Enterobacter agglomerans. The deoxyribonucleic acid base composition of isolates was 52.6 and 53.1 mol% guanine plus cytosine. Both isolates and a known strain of E. agglomerans carried out a mixed acid type of glucose fermentation. N2 fixation by E. agglomerans was inhibited by O2; consequently, N2 served as an N source only for cells growing anaerobically in media lacking a major source of combined N. However, peptone, NH4Cl, or KNO3 served as an N source under either aerobic or anaerobic conditions. It was estimated that 2 x 10(2) cells of E. agglomerans were present per termite gut. This value was 100-fold lower than expected, based on N2 fixation, low recoveries of E. agglomerans may be related to the marked decrease in N2 fixation rates observed when intact termites or their extracted guts were manipulated for the isolation of bacteria. It was concluded that the N2-fixing activity of E. agglomerans may be important to the N economy of C. formosanus.

  12. Phylogeny and identification of Pantoea species and typing of Pantoea agglomerans strains by multilocus gene sequencing.

    Science.gov (United States)

    Delétoile, Alexis; Decré, Dominique; Courant, Stéphanie; Passet, Virginie; Audo, Jennifer; Grimont, Patrick; Arlet, Guillaume; Brisse, Sylvain

    2009-02-01

    Pantoea agglomerans and other Pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of Pantoea strains and their possible association with hosts and disease remain poorly known, and identification of Pantoea species is difficult. We characterized 36 Pantoea strains, including 28 strains of diverse origins initially identified as P. agglomerans, by multilocus gene sequencing based on six protein-coding genes, by biochemical tests, and by antimicrobial susceptibility testing. Phylogenetic analysis and comparison with other species of Enterobacteriaceae revealed that the genus Pantoea is highly diverse. Most strains initially identified as P. agglomerans by use of API 20E strips belonged to a compact sequence cluster together with the type strain, but other strains belonged to diverse phylogenetic branches corresponding to other species of Pantoea or Enterobacteriaceae and to probable novel species. Biochemical characteristics such as fosfomycin resistance and utilization of d-tartrate could differentiate P. agglomerans from other Pantoea species. All 20 strains of P. agglomerans could be distinguished by multilocus sequence typing, revealing the very high discrimination power of this method for strain typing and population structure in this species, which is subdivided into two phylogenetic groups. PCR detection of the repA gene, associated with pathogenicity in plants, was positive in all clinical strains of P. agglomerans, suggesting that clinical and plant-associated strains do not form distinct populations. We provide a multilocus gene sequencing method that is a powerful tool for Pantoea species delineation and identification and for strain tracking.

  13. Pantoea agglomerans in Immunodeficient Patients with Different Respiratory Symptoms

    Directory of Open Access Journals (Sweden)

    Erika Odilia Flores Popoca

    2012-01-01

    Full Text Available The aim of this paper was to determine in 32 patients from 4 different Mexican hospitals the frequency of opportunistic bacteria in the 2010 to 2011 time period. The patients were divided in 4 groups. Group 1 included 21 HIV positive patients with acute respiratory syndrome. Four HIV positive patients with tuberculosis symptoms were included in Group 2; two patients with tuberculosis symptoms and one asymptomatic person formed Group 3. Reference Group 4 included 4 patients from whom 4 strains of Mycobacterium spp. had been reported. The strains were isolated and identified by 16S rRNA gene amplification, API 20E and 50CH, biochemical test, and antibiotic sensitivity. The strains found were 10 Pantoea agglomerans, 6 Mycobacterium spp., 6 Pseudomonas spp. and 10 strains of normal floral species: Thermoactinomycetes bacterium (1, Enterococcus faecium (2, Bacillus licheniformis (1, Lactobacillus rhamnosus (2, Streptococcus oralis (2, Streptococcus anginosus (1, and Enterobacter hormaechei (1.

  14. Pantoea agglomerans in immunodeficient patients with different respiratory symptoms.

    Science.gov (United States)

    Flores Popoca, Erika Odilia; Miranda García, Maximino; Romero Figueroa, Socorro; Mendoza Medellín, Aurelio; Sandoval Trujillo, Horacio; Silva Rojas, Hilda Victoria; Ramírez Durán, Ninfa

    2012-01-01

    The aim of this paper was to determine in 32 patients from 4 different Mexican hospitals the frequency of opportunistic bacteria in the 2010 to 2011 time period. The patients were divided in 4 groups. Group 1 included 21 HIV positive patients with acute respiratory syndrome. Four HIV positive patients with tuberculosis symptoms were included in Group 2; two patients with tuberculosis symptoms and one asymptomatic person formed Group 3. Reference Group 4 included 4 patients from whom 4 strains of Mycobacterium spp. had been reported. The strains were isolated and identified by 16S rRNA gene amplification, API 20E and 50CH, biochemical test, and antibiotic sensitivity. The strains found were 10 Pantoea agglomerans, 6 Mycobacterium spp., 6 Pseudomonas spp. and 10 strains of normal floral species: Thermoactinomycetes bacterium (1), Enterococcus faecium (2), Bacillus licheniformis (1), Lactobacillus rhamnosus (2), Streptococcus oralis (2), Streptococcus anginosus (1), and Enterobacter hormaechei (1).

  15. Bacteremia caused by Pantoea agglomerans at a medical center in Taiwan, 2000-2010.

    Science.gov (United States)

    Cheng, Aristine; Liu, Chia-Ying; Tsai, Hsih-Yeh; Hsu, Meng-Shuian; Yang, Chia-Jui; Huang, Yu-Tsung; Liao, Chun-Hsing; Hsueh, Po-Ren

    2013-06-01

    There are only three case reports of adult patients with spontaneous Pantoea agglomerans bacteremia in the English literature. The aim of this study was to investigate clinical and microbiologic characteristics patients of P agglomerans bacteremia. We studied all adult patients with P agglomerans bacteremia at a medical center from 2000 to 2010. The isolates were identified using two commercial identification systems. Of the 18 patients identified, 72% (n = 13) had active gastroesophageal disease treated with antacids. Two-thirds of patients had indwelling central lines and advanced cancers. None of the removed catheter tips yielded P agglomerans and line persistence was not associated with adverse outcomes. Initial disease severity was low, hypotension was uncommon and no patient died of bacteremia. Recurrence of bacteremia occurred in one patient with deep-seated infection. 16srRNA gene sequencing identified only half of the isolates as P agglomerans. The remaining nine isolates were Enterobacter species for six, Pantoea ananatis for two, and Exiguobacterium profundum for one. There were no significant differences between the characteristics of the subgroup molecularly identified as P agglomernas and the overall group characteristics. Eleven (61%) of the 18 isolates were susceptible to cefazolin, six (33%) susceptible to fosfomycin (MIC ≤ 64 mg/ml). Two isolates had colistin MICs ≥ 4 mg/ml. Bacteremia caused by P agglomerans is associated with gastroesophageal reflux disease and receipt of antacids. 16srRNA gene sequencing should not be used as the sole basis for its identification and we have highlighted the need for another molecular-based technique to conclusively characterize P agglomerans. Copyright © 2012. Published by Elsevier B.V.

  16. Endophytic population of Pantoea agglomerans in citrus plants and development of a cloning vector for endophytes.

    Science.gov (United States)

    Andreote, Fernando D; Rossetto, Priscilla B; Souza, Leonardo C A; Marcon, Joelma; Maccheroni, Walter; Azevedo, João L; Araújo, Welington L

    2008-10-01

    Harmless bacteria inhabiting inner plant tissues are termed endophytes. Population fluctuations in the endophytic bacterium Pantoea agglomerans associated with two species of field cultured citrus plants were monitored over a two-year period. The results demonstrated that populations of P. agglomerans fluctuated in Citrus reticulata but not C. sinensis. A cryptic plasmid pPA3.0 (2.9 kb) was identified in 35 out of 44 endophytic isolates of P. agglomerans and was subsequently sequenced. The origins of replication were identified and nine out of 18 open reading frames (ORFs) revealed homology with described proteins. Notably, two ORFs were related to cellular transport systems and plasmid maintenance. Plasmid pPA3.0 was cloned and the gfp gene inserted to generate the pPAGFP vector. The vector was introduced into P. agglomerans isolates and revealed stability was dependent on the isolate genotype, ninety-percent stability values were reached after 60 hours of bacterial cultivation in most evaluated isolates. In order to definitively establish P. agglomerans as an endophyte, the non-transformed bacterium was reintroduced into in vitro cultivated seedlings and the density of inner tissue colonization in inoculated plants was estimated by bacterium re-isolation, while the tissue niches preferred by the bacterium were investigated by scanning electronic microscopy (SEM). Cells from P. agglomerans (strain ARB18) at similar densities were re-isolated from roots, stems and leaves and colonization of parenchyma and xylem tissues were observed. Data suggested that P. agglomerans is a ubiquitous citrus endophyte harboring cryptic plasmids. These characteristics suggest the potential to use the bacterium as a vehicle to introduce new genes in host plants via endophytic bacterial transformation. (c) 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Survival of Pantoea agglomerans E325 as fire blight biocontrol agent when osmoadapted in high-saline medium

    Science.gov (United States)

    Pantoea agglomerans strain E325 is available commercially as the active ingredient in a freeze-dried product (Bloomtime FDTM) of Northwest Agricultural Products (NAP) for biological control of fire blight. Osmoadaptation, which involves the combination of Pantoea agglomerans strain E325 is available...

  18. Confirmation of the structure of lipid A from Enterobacter agglomerans by electrospray ionization tandem mass spectrometry

    Science.gov (United States)

    Boue; Cole

    2000-03-01

    Electrospray ionization (ESI) combined with tandem mass spectrometry (MS/MS) was utilized for the structural confirmation of lipid A derived from Enterobacter agglomerans, a Gram-negative bacterium commonly found in field cotton. Previous ESI-MS studies conducted in our laboratory found that similarities exist between the fatty acid side-chains in the lipid A of E. agglomerans and that of Salmonella minnesota. It was noted that heterogeneity at the fatty acyl chain at position 3' of the diglucosamine backbone of E. agglomerans can take the form of either a myristyloxymyristyl group or, less commonly, a hydroxymyristyloxymyristyl moiety. In this work, tandem mass spectra obtained from heptaacyl and hexaacyl lipid A precursors derived from E. agglomerans and a known standard S. minnesota were compared to assist in structural elucidation. These ESI-MS/MS experiments confirmed the previously reported structure for lipid A derived from E. agglomerans. Moreover, MS/MS data indicated that the additional hydroxyl group of the 3'-position hydroxymyristyloxymyristyl moiety is present as the alpha-isomer.

  19. Biological control of postharvest pear diseases using a bacterium, Pantoea agglomerans CPA-2.

    Science.gov (United States)

    Nunes, C; Usall, J; Teixidó, N; Viñas, I

    2001-10-22

    Epiphytic microorganisms isolated from the fruits and leaf surfaces of apples and pears were screened for antagonistic activity against Penicillium expansum on pears. From 247 microorganisms tested for antagonistic properties against P. expansum, a bacterium strain identified as Pantoea agglomerans (CPA-2) was selected. This bacterium was very effective against Botrytis cinerea, P. expansum and Rhizopus stolonifer. Complete control at the three tested concentrations (2 x 10(7), 8 x 10(7) and 1 x 10(8) CFU ml(-1)) was obtained on wounded pears inoculated with 10(3), 10(4) and 10(5) conidia ml(-1) of P. expansum and R. stolonifer. At 8 x 10(7) CFU ml(-1), Pan. agglomerans reduced B. cinerea decay by more than 80% at the three concentrations of the pathogen. In over 3 years of experiments in semicommercial trials, Pan. agglomerans provided excellent control against B. cinerea and P. expansum under cold storage, either in air or in low oxygen atmospheres. Equal control was obtained with Pan. agglomerans at 8 x 10(7) CFU ml(-1), as with the fungicide imazalil at commercial doses, against both pathogens. Pan. agglomerans grew well inside wounds on pears at both room and cold temperatures and under modified atmospheres. In contrast, it grew poorly on the surface of intact fruit.

  20. Growth and endotoxin production of Yersinia enterocolitica and Enterobacter agglomerans in packed erythrocytes.

    Science.gov (United States)

    Arduino, M J; Bland, L A; Tipple, M A; Aguero, S M; Favero, M S; Jarvis, W R

    1989-07-01

    Since 1987, the Centers for Disease Control investigated six cases of transfusion-associated sepsis. All six patients developed septic shock after receiving units of packed erythrocytes (PRBCs) contaminated with Yersinia enterocolitica (five patients) and Enterobacter agglomerans (one patient); three of the blood recipients died. We studied the growth and endotoxin production of Y. enterocolitica and E. agglomerans in units of PRBCs stored at 4 degrees C for 60 days. When PRBCs were inoculated with 0.1 to 1.0 CFU of these organisms per ml, both Y. enterocolitica and E. agglomerans entered log-phase growth 2 to 3 weeks after inoculation; generation times were 15 and 22 h, respectively. Endotoxin was first detected at 3 weeks following inoculation, and the concentration paralleled the log phase of growth of the strains tested. These data show that prolonged storage of PRBCs at 4 degrees C provides conditions that allow these two organisms to grow and subsequently produce high concentrations of endotoxin.

  1. Draft genome sequences of Pantoea agglomerans and Pantoea vagans isolates associated with termites

    DEFF Research Database (Denmark)

    Palmer, Marike; de Maayer, Pieter; Thomas-Poulsen, Michael

    2016-01-01

    The genus Pantoea incorporates many economically and clinically important species. The plant-associated species, Pantoea agglomerans and Pantoea vagans, are closely related and are often isolated from similar environments. Plasmids conferring certain metabolic capabilities are also shared amongst...... these two species. The genomes of two isolates obtained from fungus-growing termites in South Africa were sequenced, assembled and annotated. A high number of orthologous genes are conserved within and between these species. The difference in genome size between P. agglomerans MP2 (4,733,829 bp) and P....... vagans MP7 (4,598,703 bp) can largely be attributed to the differences in plasmid content. The genome sequences of these isolates may shed light on the common traits that enable P. agglomerans and P. vagans to co-occur in plant- and insect-associated niches....

  2. Nosocomial outbreak of Pantoea agglomerans in a pediatric urgent care center

    Directory of Open Access Journals (Sweden)

    Eliana L. Bicudo

    Full Text Available Pantoea agglomerans is a Gram-negative bacterium whose isolates can be found in blood cultures and other secretions. This article described one outbreak of sepsis due to this bacterium in the pediatric urgent care center of a tertiary hospital, in Brasilia, Federal District. This was a case-control study and it evaluated the risk factors for acquisition of nosocomial sepsis caused by Pantoea agglomerans. Six case-patients and 15 control-patients have been found. The risk factors were: vomiting in the internment and presence of solution compositions in the intravenous hydration therapy received in bigger number than 2 compositions. The transference tube used for the compositions was contaminated with Pantoea agglomerans, characterizing the outbreak with common source. The evolution of all the patients was favorable.

  3. Draft genome sequences of Pantoea agglomerans and Pantoea vagans isolates associated with termites.

    Science.gov (United States)

    Palmer, Marike; de Maayer, Pieter; Poulsen, Michael; Steenkamp, Emma T; van Zyl, Elritha; Coutinho, Teresa A; Venter, Stephanus N

    2016-01-01

    The genus Pantoea incorporates many economically and clinically important species. The plant-associated species, Pantoea agglomerans and Pantoea vagans, are closely related and are often isolated from similar environments. Plasmids conferring certain metabolic capabilities are also shared amongst these two species. The genomes of two isolates obtained from fungus-growing termites in South Africa were sequenced, assembled and annotated. A high number of orthologous genes are conserved within and between these species. The difference in genome size between P. agglomerans MP2 (4,733,829 bp) and P. vagans MP7 (4,598,703 bp) can largely be attributed to the differences in plasmid content. The genome sequences of these isolates may shed light on the common traits that enable P. agglomerans and P. vagans to co-occur in plant- and insect-associated niches.

  4. DETERMINAÇÃO DE ISOFLAVONAS NAS DIVERSAS ETAPAS DO PROCESSAMENTO DO “IOGURTE” DE SOJA

    Directory of Open Access Journals (Sweden)

    E. A. ROSSI

    2008-09-01

    Full Text Available

    As isoflavonas presentes nos grãos de soja, substâncias capazes de exercer vários efeitos benéficos à saúde, entre eles o de reduzir os níveis de lípides séricos, podem ser sensivelmente afetadas pelas condições de processamento. Portanto, o objetivo do presente estudo foi quantificar as isoflavonas após as diversas etapas do processamento de um novo produto probiótico de soja fermentado com Enterococcus faecium eLactobacillus jugurti, cuja ação hipocolesterolêmica já foi anteriormente demonstrada. As isoflavonas foram extraídas com mistura de metanol e ácido clorídrico (4:1 e quantificadas por cromatografia líquida de alta eficiência com coluna C18. As amostras foram coletadas em 10 etapas distintas do processo de obtenção do “iogurte” de soja. Os resultados mostraram que as isoflavonas glicosídicas apareceram em maior concentração em todas as amostras analisadas e que o processo de transformação da soja em “iogurte” ocasionou uma diminuição considerável de todas as formas de isoflavonas (glicosídicas e agliconas fazendo com que o produto acabado apresentasse uma concentração cerca de 13 vezes menor que a verificada no grão “in natura”. O processo de fermentação não foi efetivo no sentido de promover a hidrólise das isoflavonas glicosídicas.

  5. Sugarcane growth promotion by the endophytic bacterium Pantoea agglomerans 33.1.

    Science.gov (United States)

    Quecine, M C; Araújo, W L; Rossetto, P B; Ferreira, A; Tsui, S; Lacava, P T; Mondin, M; Azevedo, J L; Pizzirani-Kleiner, A A

    2012-11-01

    The promotion of sugarcane growth by the endophytic Pantoea agglomerans strain 33.1 was studied under gnotobiotic and greenhouse conditions. The green fluorescent protein (GFP)-tagged strain P. agglomerans 33.1::pNKGFP was monitored in vitro in sugarcane plants by microscopy, reisolation, and quantitative PCR (qPCR). Using qPCR and reisolation 4 and 15 days after inoculation, we observed that GFP-tagged strains reached similar density levels both in the rhizosphere and inside the roots and aerial plant tissues. Microscopic analysis was performed at 5, 10, and 18 days after inoculation. Under greenhouse conditions, P. agglomerans 33.1-inoculated sugarcane plants presented more dry mass 30 days after inoculation. Cross-colonization was confirmed by reisolation of the GFP-tagged strain. These data demonstrate that 33.1::pNKGFP is a superior colonizer of sugarcane due to its ability to colonize a number of different plant parts. The growth promotion observed in colonized plants may be related to the ability of P. agglomerans 33.1 to synthesize indoleacetic acid and solubilize phosphate. Additionally, this strain may trigger chitinase and cellulase production by plant roots, suggesting the induction of a plant defense system. However, levels of indigenous bacterial colonization did not vary between inoculated and noninoculated sugarcane plants under greenhouse conditions, suggesting that the presence of P. agglomerans 33.1 has no effect on these communities. In this study, different techniques were used to monitor 33.1::pNKGFP during sugarcane cross-colonization, and our results suggested that this plant growth promoter could be used with other crops. The interaction between sugarcane and P. agglomerans 33.1 has important benefits that promote the plant's growth and fitness.

  6. Isolation of an Enterobacter agglomerans strain with inhibitory activity on Pseudomonas aeruginosa.

    Science.gov (United States)

    Moujir, L

    1992-01-01

    Antagonism between Enterobacter agglomerans and Pseudomonas aeruginosa was due to an extracellular substance produced by E. agglomerans which accumulates in the culture medium. This substance was also toxic to Escherichia coli and Staphylococcus albus and its MIC for P. aeruginosa ranged between 7.8 and 3.9 micrograms/ml. However, when the extracts from the culture medium were irradiated from 2 h with white light (15 mW/cm2), the MIC was lower (3.9-1.9 micrograms/ml) suggesting that the active substance was a phototoxin.

  7. Plant-isolated Pantoea agglomerans--new look into potential pathogenicity.

    Science.gov (United States)

    Mudryk, M

    2012-01-01

    Pantoea agglomerans strains have been isolated from the surface of different edible plants which are major ingredients of traditional foods of the Black Sea region countries. Bacterial strains did not possess their pathogenic properties when tested routinely in vitro, but evinced the resistance to broad-spectrum antibiotics. Experiments on murine model (BALB/c mice) have demonstrated the ability of P. agglomerans to penetrate into internal organs and provoke the distinct dose-dependent physiological changes in the intestine and gut associated lymphoid tissues (GALT).

  8. Chitinolytic Enterobacter agglomerans Antagonistic to Fungal Plant Pathogens.

    Science.gov (United States)

    Chernin, L; Ismailov, Z; Haran, S; Chet, I

    1995-05-01

    Three Enterobacter agglomerans strains which produce and excrete proteins with chitinolytic activity were found while screening soil-borne bacteria antagonistic to fungal plant pathogens. The chitinolytic activity was induced when the strains were grown in the presence of colloidal chitin as the sole carbon source. It was quantitated by using assays with chromogenic p-nitrophenyl analogs of disaccharide, trisaccharide, and tetrasaccharide derivatives of N-acetylglucosamine. A set of three fluorescent substrates with a 4-methylumbelliferyl group linked by (beta)-1,4 linkage to N-acetylglucosamine mono- or oligosaccharides were used to identify the chitinolytic activities of proteins which had been renatured following their separation by electrophoresis. This study provides the most complete evidence for the presence of a complex of chitinolytic enzymes in Enterobacter strains. Four enzymes were detected: two N-acetyl-(beta)-d-glucosaminidases of 89 and 67 kDa, an endochitinase with an apparent molecular mass of 59 kDa, and a chitobiosidase of 50 kDa. The biocontrol ability of the chitinolytic strains was demonstrated under greenhouse conditions. The bacteria decreased the incidence of disease caused by Rhizoctonia solani in cotton by 64 to 86%. Two Tn5 mutants of one of the isolates, which were deficient in chitinolytic activity, were unable to protect plants against the disease.

  9. Purification and partial characterization of azoreductase from Enterobacter agglomerans.

    Science.gov (United States)

    Moutaouakkil, Adnane; Zeroual, Youssef; Zohra Dzayri, Fatima; Talbi, Mohamed; Lee, Kangmin; Blaghen, Mohamed

    2003-05-01

    Azoreductase, an enzyme catalyzing the reductive cleavage of the azo bond of methyl red (MR) and related dyes, was purified to electrophoretic homogeneity from Enterobacter agglomerans. This bacterial strain, isolated from dye-contaminated sludge, has a higher ability to grow, under aerobic conditions, on culture medium containing 100mg/L of MR. The enzyme was purified approximately 90-fold with 20% yield by ammonium sulfate precipitation, followed by three steps of column chromatography (gel-filtration, anion-exchange, and dye-affinity). The purified enzyme is a monomer with a molecular weight of 28,000 Da. The maximal azoreductase activity was observed at pH 7.0 and at 35 degrees C. This activity was NADH dependent. The K(m) values for both NADH and MR were 58.9 and 29.4 microM, respectively. The maximal velocity (V(max)) was 9.2 micromol of NADH min(-1)mg(-1). The purified enzyme is inhibited by several metal ions including Fe(2+) and Cd(2+).

  10. Aplikasi Rizobakteri Pantoea agglomerans untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Jagung (Zea mays, L. Varietas Hibrida BISI-2

    Directory of Open Access Journals (Sweden)

    I GUSTI NGURAH RAKA

    2013-08-01

    Full Text Available This experiment was conducted in the village of Sibang Gede, District of Abiansemal, BadungRegency on November 2011 until February 2012. The research objective is to enhance the growth andyield of maize hybrid BISI-2 using rhizobacteria Pantoea agglomerans as PGPR in compostformulations. This study used a randomized block design (RBD single factor with 5 treatments and 5replications. The treatments tested were P. agglomerans BS7a, P. agglomerans BS7b, P. agglomeransBS2a, and P. agglomerans BS5a. All treatments P. agglomerans formulated in the form of compostand given a dose of 50 g/hole. As a control dose of compost 50 g/hole without PGPR. The resultsshowed that the application of rhizobacteria P. agglomerans increased the maximum plant height, netassimilation rate, crop growth rate, chlorophyll content of leaves, fresh weight of roots per plant, ovendried weight of stover per plant, number of seeds per plant, cob length, cob diameter, seed weightmoisture content of 12% per plant and seed yield of 12% water content per hectare.

  11. Molecular epidemiology of a nosocomial outbreak due to Enterobacter cloacae and Enterobacter agglomerans in Campinas, São Paulo, Brazil Epidemiologia molecular de um surto de bacteriemia por Enterobacter cloacae e Enterobacter agglomerans ocorrido na região de Campinas, São Paulo, Brasil

    Directory of Open Access Journals (Sweden)

    Célia R. GONÇALVES

    2000-02-01

    Full Text Available A total of 73 isolates (57 Enterobacter cloacae and 16 Enterobacter agglomerans, recovered during an outbreak of bacteremia in the Campinas area, São Paulo, Brazil, were studied. Of these isolates, 61 were from parenteral nutrition solutions, 9 from blood cultures, 2 from a sealed bottle of parenteral nutrition solution, and one was of unknown origin. Of the 57 E. cloacae isolates, 54 were biotype 26, two were biotype 66 and one was non-typable. Of 39 E. cloacae isolates submitted to ribotyping, 87.2% showed the same banding pattern after cleavage with EcoRI and BamHI. No important differences were observed in the antimicrobial susceptibility patterns among E. cloacae isolates exhibiting the same biotype, serotype and ribotype. All E. agglomerans isolates, irrespective of their origin, showed same patterns when cleaved with EcoRI and BamHI. The results of this investigation suggest an intrinsic contamination of parenteral nutrition solutions and incriminate these products as a vehicle of infection in this outbreak.Foram estudadas um total de 73 cepas (57 de E. cloacae e 16 E. agglomerans, isoladas durante um surto de bacteriemia ocorrido na região de Campinas, S. Paulo. Entre estas cepas, 61 foram isoladas de solução de nutrição parenteral, 9 de sangue, 2 de bolsa fechada de solução de nutrição parenteral e uma era de origem desconhecida. Entre as 57 cepas de E. cloacae, a maioria das cepas foram do biotipo 26/sorotipo O3 (39 cepas e do biotipo 26/OR (13. Entre as 39 cepas de E. cloacae ribotipadas, 87,2% apresentaram o mesmo padrão de bandas com EcoRI e BamHI. Cepas de E. cloacae pertencentes ao mesmo biotipo, sorotipo e ribotipo não apresentaram diferenças significativas em relação ao padrão de sensibilidade aos agentes antimicrobianos. Todas as cepas de E. agglomerans, independente da origem, pertenciam ao mesmo ribotipo após a clivagem com EcoRI e BamHI. Os resultados obtidos sugerem uma contaminação intrínseca das solu

  12. Pengaruh Aplikasi Formula Pantoea agglomerans Terhadap Aktivitas Antioksidan dan Kandungan Klorofil Daun Tanaman Strowberi

    Directory of Open Access Journals (Sweden)

    TRISNA AGUNG PHABIOLA

    2014-01-01

    Full Text Available The objectives of this experiment were conducted to evaluate the effectiveness of Pantoeaagglomerans formulations to increase antioxidant activities and chlorophyll content of strawberry plants.P. agglomerans was formulated in the forms of gel and powder. Measurement of peroxidase activitycarried out 7 days after application of P. agglomerans formulations on strawberry plants. The mannerin which the Cohen prosedure suggested by Simon and Rose (1970 and has been modified. Totalchlorophyll content (SPAD unit was determined with a chlorophyll-meter SPAD-502. Some growthparameters were observed such as plant height, number of leaves, and leaf area. Results of this studyshowed that application of P. agglomerans formulations could increase the peroxidase activity. Peroxidaseactivity on treated plants increased by 47.17% to 50.16% in comparison with un-treated plants. Totalchlorophyll content on treated plants increased by 23.81% to 28.22% in comparison with un-treatedplants. These results suggested that application of P. agglomerans formulations could increase the totalchlorophyll content and antioxidant activity of strawberry plants.

  13. Draft Genome Sequence of the Phyllosphere Model Bacterium Pantoea agglomerans 299R

    NARCIS (Netherlands)

    Remus-Emsermann, M.N.P.; Kim, E.B.; Marco, M.L.; Tecon, R.; Leveau, J.H.J.

    2013-01-01

    Bacteria belonging to the genus Pantoea are common colonizers of plant leaf surfaces. Here, we present the draft genome sequence of Pantoea agglomerans 299R, a phyllosphere isolate that has become a model strain for studying the ecology of plant leaf-associated bacterial commensals.

  14. Immigrant Pantoea agglomerans embedded within indigenous microbial aggregates: a novel spatial distribution of epiphytic bacteria.

    Science.gov (United States)

    Yu, Qing; Ma, Anzhou; Cui, Mengmeng; Zhuang, Xuliang; Zhuang, Guoqiang

    2014-02-01

    Immigrant bacteria located on leaf surfaces are important to the health of plants as well as to people who consume fresh fruits and vegetables. However, the spatial distribution and organization of these immigrant bacteria on leaf surfaces are still poorly understood. To examine the spatial organization of these strains, two bacterial strains on tobacco leaves: (1) an indigenous strain, Pseudomonas stutzeri Nov. Y2011 labeled with green fluorescent protein, and (2) an immigrant strain Pantoea agglomerans labeled with cyan fluorescent protein isolated from pear, were studied. Under moist conditions, P. agglomerans cells quickly disappeared from direct observation by laser-scanning confocal microscopy, although elution results indicated that large amounts of live cells were still present on the leaves. Following exposure to desiccation stress, particles of cyan fluorescent protein-labeled P. agglomerans were visible within cracked aggregates of P. stutzeri Nov. Y2011. Detailed observation of sectioned aggregates showed that colonies of immigrant P. agglomerans were embedded within aggregates of P. stutzeri Nov. Y2011. Furthermore, carbon-resource partitioning studies suggested that these two species could coexist without significant nutritional competition. This is the first observation of an immigrant bacterium embedding within aggregates of indigenous bacteria on leaves to evade harsh conditions in the phyllosphere.

  15. Unusual causes of peritonitis in a peritoneal dialysis patient: Alcaligenes faecalis and Pantoea agglomerans

    Directory of Open Access Journals (Sweden)

    Arikan Hakki

    2011-04-01

    Full Text Available Abstract An 87 -year-old female who was undergoing peritoneal dialysis presented with peritonitis caused by Alcaligenes faecalis and Pantoea agglomerans in consecutive years. With the following report we discuss the importance of these unusual microorganisms in peritoneal dialysis patients.

  16. Isolation and characterization of Carotenoid biosynthesis genes from Pantoea agglomerans pv. millettiae Wist 801

    International Nuclear Information System (INIS)

    Hirata, R.; Tominaga, H.; Kamiunten, H.

    2007-01-01

    Pantoea agglomerans pv. millettiae produces a yellow pigment and is the causal agent of wisteria stem galls. We cloned and sequenced a 6.8-kb DNA fragment containing the yellow pigment gene. Analysis of the nucleotide sequence revealed that this clone contains five open reading frames (ORFs) transcribed in the same direction, and one terminal ORF transcribed in the opposite orientation. Comparison of the nucleotide and predicted amino acid sequences of these ORFs showed that they correspond to the carotenoid biosynthesis (crt) genes crtE, -X, -Y, -I, -B and -Z, respectively, in P. ananatis pv. uredovora 20D3, and P. agglomerans strains Eho 10 and Eho 13. It was suggested that the carotenoid biosynthesis pathway of P. agglomerans pv. millettiae is basically identical to that of the three other Pantoea strains. When the crt genes from P. agglomerans pv. millettiae were expressed in E. coli WP2, this bacterium also displayed a yellow phenotype. The carotenoid pigments are known for their ability to protect various organisms against UV-induced damage, however, there were no significant differences in mutagenesis and survival after UV irradiation between yellow-pigmented and non-pigmented E. coli WP2 strains

  17. Antibiosis by Pantoea agglomerans biocontrol strain E325 against Erwinia amylovora on apple blossom stigmas

    Science.gov (United States)

    Pantoea agglomerans E325, the active ingredient in a commercial product for fire blight control, was previously shown in vitro to produce a unique alkaline- and phosphate-sensitive antibiotic specific to Erwinia amylovora. Antibiosis was evaluated as a mode of antagonism on blossom stigmas using two...

  18. Unusual causes of peritonitis in a peritoneal dialysis patient: Alcaligenes faecalis and Pantoea agglomerans

    Science.gov (United States)

    2011-01-01

    An 87 -year-old female who was undergoing peritoneal dialysis presented with peritonitis caused by Alcaligenes faecalis and Pantoea agglomerans in consecutive years. With the following report we discuss the importance of these unusual microorganisms in peritoneal dialysis patients. PMID:21477370

  19. Controlled release of Pantoea agglomerans E325 for biocontrol of fire blight

    Science.gov (United States)

    Microencapsulation and controlled release of Pantoea agglomerans strain E325 (E325), which is an antagonist to bacterial pathogen (Erwinia amylovora) of fire blight, a devastating disease of apple and pear, have been investigated. Uniform core-shell alginate microcapsules (AMCs), 60-300 µm in diamet...

  20. Phylogeny and Identification of Pantoea Species and Typing of Pantoea agglomerans Strains by Multilocus Gene Sequencing ▿ †

    OpenAIRE

    Delétoile, Alexis; Decré, Dominique; Courant, Stéphanie; Passet, Virginie; Audo, Jennifer; Grimont, Patrick; Arlet, Guillaume; Brisse, Sylvain

    2008-01-01

    Pantoea agglomerans and other Pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of Pantoea strains and their possible association with hosts and disease remain poorly known, and identification of Pantoea species is difficult. We characterized 36 Pantoea strains, including 28 strains of diverse origins initially identified as P. agglomerans, by multilocus gene sequencing based on six protein-coding genes, by biochemical tests, and by antimicrobial ...

  1. Engineering the lycopene synthetic pathway in E. coli by comparison of the carotenoid genes of Pantoea agglomerans and Pantoea ananatis.

    Science.gov (United States)

    Yoon, Sang-Hwal; Kim, Ju-Eun; Lee, Sook-Hee; Park, Hye-Min; Choi, Myung-Suk; Kim, Jae-Yean; Lee, Si-Hyoung; Shin, Yong-Chul; Keasling, Jay D; Kim, Seon-Won

    2007-02-01

    The lycopene synthetic pathway was engineered in Escherichia coli using the carotenoid genes (crtE, crtB, and crtI) of Pantoea agglomerans and Pantoea ananatis. E. coli harboring the P. agglomerans crt genes produced 27 mg/l of lycopene in 2YT medium without isopropyl-beta-D: -thiogalactopyranoside (IPTG) induction, which was twofold higher than that produced by E. coli harboring the P. ananatis crt genes (12 mg/l lycopene) with 0.1 mM IPTG induction. The crt genes of P. agglomerans proved better for lycopene production in E. coli than those of P. ananatis. The crt genes of the two bacteria were also compared in E. coli harboring the mevalonate bottom pathway, which was capable of providing sufficient carotenoid building blocks, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), with exogenous mevalonate supplementation. Lycopene production significantly increased using the mevalonate bottom pathway and 60 mg/l of lycopene was obtained with the P. agglomerans crt genes, which was higher than that obtained with the P. ananatis crt genes (35 mg/l lycopene). When crtE among the P. ananatis crt genes was replaced with P. agglomerans crtE or Archaeoglobus fulgidus gps, both lycopene production and cell growth were similar to that obtained with P. agglomerans crt genes. The crtE gene was responsible for the observed difference in lycopene production and cell growth between E. coli harboring the crt genes of P. agglomerans and P. ananatis. As there was no significant difference in lycopene production between E. coli harboring P. agglomerans crtE and A. fulgidus gps, farnesyl diphosphate (FPP) synthesis was not rate-limiting in E. coli.

  2. Induction of salt tolerance and up-regulation of aquaporin genes in tropical corn by rhizobacterium Pantoea agglomerans.

    Science.gov (United States)

    Gond, S K; Torres, M S; Bergen, M S; Helsel, Z; White, J F

    2015-04-01

    Bacteria were isolated from surface disinfected seeds of eight modern corn types and an ancestor of corn, 'teosinte' and identified using 16S rDNA sequences. From each of the modern corn types we obtained Bacillus spp. (including, Bacillus amyloliquefaciens and Bacillus subtilis); while from teosinte we obtained only Pantoea agglomerans and Agrobacterium species. Of these bacteria, only P. agglomerans could actively grow under hypersaline conditions and increase salt tolerance of tropical corn seedlings. In laboratory and greenhouse experiments where plants were watered with a 0.2 mol l(-1) NaCl solution, P. agglomerans was found to enhance the capacity of tropical corn to grow compared to uninoculated controls. The total dry biomass was significantly higher in P. agglomerans-treated plants compared to controls under saline water. Gene expression analysis showed the up-regulation of the aquaporin gene family especially plasma membrane integral protein (ZmPIP) genes in P. agglomerans-treated plants. The plasma membrane integral protein type 2 (PIP2-1) gene in tropical corn seedlings was highly up-regulated by P. agglomerans treatment under salt stress conditions. Microscopic examination of P. agglomerans inoculated seedlings revealed that the bacterium colonized root meristems densely, and as roots developed, the bacterium became sparsely located in cell junctions. The enhancement of salt tolerance capacity in tropical corn, an important food crop, has the capacity to increase its cultivation area and yield in saline soils. The application of rhizobacteria to improve salt tolerance of tropical corn is ecofriendly and cost effective. We show that P. agglomerans isolated from teosinte (an ancestor of corn) induces salt tolerance in tropical corn and up-regulation of aquaporin genes. This study shows that microbes that increase salt tolerance may be used to enhance crop growth in saline soils. © 2014 The Society for Applied Microbiology.

  3. Phylogeny and Identification of Pantoea Species and Typing of Pantoea agglomerans Strains by Multilocus Gene Sequencing ▿ †

    Science.gov (United States)

    Delétoile, Alexis; Decré, Dominique; Courant, Stéphanie; Passet, Virginie; Audo, Jennifer; Grimont, Patrick; Arlet, Guillaume; Brisse, Sylvain

    2009-01-01

    Pantoea agglomerans and other Pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of Pantoea strains and their possible association with hosts and disease remain poorly known, and identification of Pantoea species is difficult. We characterized 36 Pantoea strains, including 28 strains of diverse origins initially identified as P. agglomerans, by multilocus gene sequencing based on six protein-coding genes, by biochemical tests, and by antimicrobial susceptibility testing. Phylogenetic analysis and comparison with other species of Enterobacteriaceae revealed that the genus Pantoea is highly diverse. Most strains initially identified as P. agglomerans by use of API 20E strips belonged to a compact sequence cluster together with the type strain, but other strains belonged to diverse phylogenetic branches corresponding to other species of Pantoea or Enterobacteriaceae and to probable novel species. Biochemical characteristics such as fosfomycin resistance and utilization of d-tartrate could differentiate P. agglomerans from other Pantoea species. All 20 strains of P. agglomerans could be distinguished by multilocus sequence typing, revealing the very high discrimination power of this method for strain typing and population structure in this species, which is subdivided into two phylogenetic groups. PCR detection of the repA gene, associated with pathogenicity in plants, was positive in all clinical strains of P. agglomerans, suggesting that clinical and plant-associated strains do not form distinct populations. We provide a multilocus gene sequencing method that is a powerful tool for Pantoea species delineation and identification and for strain tracking. PMID:19052179

  4. Pantoea agglomerans: a mysterious bacterium of evil and good. Part IV. Beneficial effects.

    Science.gov (United States)

    Dutkiewicz, Jacek; Mackiewicz, Barbara; Lemieszek, Marta Kinga; Golec, Marcin; Milanowski, Janusz

    2016-06-02

    Pantoea agglomerans, a gammaproteobacterium of plant origin, possesses many beneficial traits that could be used for the prevention and/or treatment of human and animal diseases, combating plant pathogens, promotion of plant growth and bioremediation of the environment. It produces a number of antibiotics (herbicolin, pantocins, microcin, agglomerins, andrimid, phenazine, among others) which could be used for combating plant, animal and human pathogens or for food preservation. Japanese researchers have demonstrated that the low-molecular-mass lipopolysaccharide of P. agglomerans isolated by them and described as 'Immunopotentiator from Pantoea agglomerans 1 (IP-PA1)' reveals the extremely wide spectrum of healing properties, mainly due to its ability for the maintenance of homeostasis by macrophage activation. IP-PA1 was proved to be effective in the prevention and treatment of a broad range of human and animal disorders, such as tumours, hyperlipidaemia, diabetes, ulcer, various infectious diseases, atopic allergy and stress-induced immunosuppression; it also showed a strong analgesic effect. It is important that most of these effects could be achieved by the safe oral administration of IP-PA1. Taking into account that P. agglomerans occurs commonly as a symbiont of many species of insects, including mosquitoes transmitting the Plasmodium parasites causing malaria, successful attempts were made to apply the strategy of paratransgenesis, in which bacterial symbionts are genetically engineered to express and secrete anti-Plasmodium effector proteins. This strategy shows prospects for a successful eradication of malaria, a deadly disease killing annually over one million people, as well as of other vector-borne diseases of humans, animals and plants. Pantoea agglomerans has been identified as an antagonist of many plant pathogens belonging to bacteria and fungi, as a result of antibiotic production, competition mechanisms or induction of plant resistance. Its use as

  5. Pantoea agglomerans: a mysterious bacterium of evil and good. Part IV. Beneficial effects

    Directory of Open Access Journals (Sweden)

    Jacek Dutkiewicz

    2016-06-01

    Full Text Available [i][/i][i]Pantoea agglomerans[/i], a gammaproteobacterium of plant origin, possesses many beneficial traits that could be used for the prevention and/or treatment of human and animal diseases, combating plant pathogens, promotion of plant growth and bioremediation of the environment. It produces a number of antibiotics (herbicolin, pantocins, microcin, agglomerins, andrimid, phenazine, among others which could be used for combating plant, animal and human pathogens or for food preservation. Japanese researchers have demonstrated that the low-molecular-mass lipopolysaccharide of [i]P. agglomerans[/i] isolated by them and described as ‘Immunopotentiator from [i]Pantoea agglomerans[/i] 1 (IP-PA1’ reveals the extremely wide spectrum of healing properties, mainly due to its ability for the maintenance of homeostasis by macrophage activation. IP-PA1 was proved to be effective in the prevention and treatment of a broad range of human and animal disorders, such as tumours, hyperlipidaemia, diabetes, ulcer, various infectious diseases, atopic allergy and stress-induced immunosuppression; it also showed a strong analgesic effect. It is important that most of these effects could be achieved by the safe oral administration of IP-PA1. Taking into account that [i]P. agglomerans[/i] occurs commonly as a symbiont of many species of insects, including mosquitoes transmitting the [i]Plasmodium[/i] parasites causing malaria, successful attempts were made to apply the strategy of paratransgenesis, in which bacterial symbionts are genetically engineered to express and secrete anti-[i]Plasmodium[/i] effector proteins. This strategy shows prospects for a successful eradication of malaria, a deadly disease killing annually over one million people, as well as of other vector-borne diseases of humans, animals and plants. [i]Pantoea agglomerans[/i] has been identified as an antagonist of many plant pathogens belonging to bacteria and fungi, as a result of antibiotic

  6. Pantoea agglomerans : a mysterious bacterium of evil and good. Part IV. Beneficial effects

    Directory of Open Access Journals (Sweden)

    Jacek Dutkiewicz

    2016-06-01

    Full Text Available Pantoea agglomerans , a gammaproteobacterium of plant origin, possesses many beneficial traits that could be used for the prevention and/or treatment of human and animal diseases, combating plant pathogens, promotion of plant growth and bioremediation of the environment. It produces a number of antibiotics (herbicolin, pantocins, microcin, agglomerins, andrimid, phenazine, among others which could be used for combating plant, animal and human pathogens or for food preservation. Japanese researchers have demonstrated that the low-molecular-mass lipopolysaccharide of P. agglomerans isolated by them and described as ‘Immunopotentiator from Pantoea agglomerans 1 (IP-PA1’ reveals the extremely wide spectrum of healing properties, mainly due to its ability for the maintenance of homeostasis by macrophage activation. IP-PA1 was proved to be effective in the prevention and treatment of a broad range of human and animal disorders, such as tumours, hyperlipidaemia, diabetes, ulcer, various infectious diseases, atopic allergy and stress-induced immunosuppression; it also showed a strong analgesic effect. It is important that most of these effects could be achieved by the safe oral administration of IP-PA1. Taking into account that P. agglomerans occurs commonly as a symbiont of many species of insects, including mosquitoes transmitting the Plasmodium parasites causing malaria, successful attempts were made to apply the strategy of paratransgenesis, in which bacterial symbionts are genetically engineered to express and secrete anti- Plasmodium effector proteins. This strategy shows prospects for a successful eradication of malaria, a deadly disease killing annually over one million people, as well as of other vector-borne diseases of humans, animals and plants. Pantoea agglomerans has been identified as an antagonist of many plant pathogens belonging to bacteria and fungi, as a result of antibiotic production, competition mechanisms or induction of

  7. Pantoea agglomerans : a mysterious bacterium of evil and good. Part III. Deleterious effects: infections of humans, animals and plants

    Directory of Open Access Journals (Sweden)

    Jacek Dutkiewicz

    2016-06-01

    Full Text Available Pantoea agglomerans , a bacterium associated with plants, is not an obligate infectious agent in humans. However, it could be a cause of opportunistic human infections, mostly by wound infection with plant material, or as a hospital-acquired infection, mostly in immunocompromised individuals. Wound infection with P. agglomerans usually follow piercing or laceration of skin with a plant thorn, wooden splinter or other plant material and subsequent inoculation of the plant-residing bacteria, mostly during performing of agricultural occupations and gardening, or children playing. Septic arthritis or synovitis appears as a common clinical outcome of exogenous infection with P. agglomerans , others include endophthalmitis, periostitis, endocarditis and osteomyelitis. Another major reason for clinical infection with P. agglomerans is exposure of hospitalized, often immunodeficient individuals to medical equipment or fluids contaminated with this bacterium. Epidemics of nosocomial septicemia with fatal cases have been described in several countries, both in adult and paediatric patients. In most cases, however, the clinical course of the hospital-acquired disease was mild and application of the proper antibiotic treatment led to full recovery. Compared to humans, there are only few reports on infectious diseases caused by Pantoea agglomerans in vertebrate animals. This species has been identified as a possible cause of equine abortion and placentitis and a haemorrhagic disease in dolphin fish ( Coryphaena hippurus . P. agglomerans strains occur commonly, usually as symbionts, in insects and other arthropods. Pantoea agglomerans usually occurs in plants as an epi- or endophytic symbiont, often as mutualist. Nevertheless, this species has also also been identified as a cause of diseases in a range of cultivable plants, such as cotton, sweet onion, rice, maize, sorghum, bamboo, walnut, an ornamental plant called Chinese taro ( Alocasia cucullata , and a

  8. Pantoea agglomerans: a mysterious bacterium of evil and good. Part III. Deleterious effects: infections of humans, animals and plants.

    Science.gov (United States)

    Dutkiewicz, Jacek; Mackiewicz, Barbara; Kinga Lemieszek, Marta; Golec, Marcin; Milanowski, Janusz

    2016-06-02

    Pantoea agglomerans, a bacterium associated with plants, is not an obligate infectious agent in humans. However, it could be a cause of opportunistic human infections, mostly by wound infection with plant material, or as a hospital-acquired infection, mostly in immunocompromised individuals. Wound infection with P. agglomerans usually follow piercing or laceration of skin with a plant thorn, wooden splinter or other plant material and subsequent inoculation of the plant-residing bacteria, mostly during performing of agricultural occupations and gardening, or children playing. Septic arthritis or synovitis appears as a common clinical outcome of exogenous infection with P. agglomerans, others include endophthalmitis, periostitis, endocarditis and osteomyelitis. Another major reason for clinical infection with P. agglomerans is exposure of hospitalized, often immunodeficient individuals to medical equipment or fluids contaminated with this bacterium. Epidemics of nosocomial septicemia with fatal cases have been described in several countries, both in adult and paediatric patients. In most cases, however, the clinical course of the hospital-acquired disease was mild and application of the proper antibiotic treatment led to full recovery. Compared to humans, there are only few reports on infectious diseases caused by Pantoea agglomerans in vertebrate animals. This species has been identified as a possible cause of equine abortion and placentitis and a haemorrhagic disease in dolphin fish (Coryphaena hippurus). P. agglomerans strains occur commonly, usually as symbionts, in insects and other arthropods. Pantoea agglomerans usually occurs in plants as an epi- or endophytic symbiont, often as mutualist. Nevertheless, this species has also also been identified as a cause of diseases in a range of cultivable plants, such as cotton, sweet onion, rice, maize, sorghum, bamboo, walnut, an ornamental plant called Chinese taro (Alocasia cucullata), and a grass called onion couch

  9. CIUDADES INTELIGENTES COMO ESPACIOS DE INTEGRACIÓN PARA INDIVIDUOS CON CAPACIDADES DIVERSAS

    Directory of Open Access Journals (Sweden)

    Luisenia Fernández O.

    2014-07-01

    Full Text Available Hoy en día las Tecnologías de Información y Comunicación (TIC penetran en el entramado social y sobre él se configura la sociedad. Esta investigación describe el uso de las TIC en ciudades que distan desemejarse de la urbe tradicional gracias al empleo racional de las tecnologías que hacen los entornos más inteligentes, logrando cerrarlas brechas existentes entre sus usuario; generando el confort de aquellos cuyas capacidades son diversas en la diversidad o con capacidades disminuidas. Es decir, estas ciudades ponen a disposición de sus pobladores todos sus recursos tecnológicos disponibles y sus aportes al servicio de sus habitantes. La metodología utilizada para esta investigación fue de tipo exploratorio- descriptivo en la cual se intenta mostrar el uso de las tecnologías (TIC y la transformación del entorno, así como sus bondades en pro de la incorporación de los ciudadanos con capacidades diversas como ciudadanos de primera, a través de la construcción de espacios que aprovechan las aportaciones de las TIC para hacer del medio ambiente un espacio inteligente para la inclusión de los vulnerables cuyo fin último no es otro que el bienestar común. Se concluye que las denominadas ciudades inteligentes son hoy en día urbes que combinan la interconectividad de redes para la confortabilidad del ciudadano con capacidades diversas; éstas procuran el equilibrio, el desarrollo, la inclusión y el bienestar de los todos individuos con capacidades disímiles en la diversidad.

  10. Familias en el siglo xxi: realidades diversas y políticas públicas

    OpenAIRE

    Karla Flores Celis

    2012-01-01

    En este texto se sintetizan las intervenciones que se realizaron durante la presentación del libro Familias en el siglo xxi: realidades diversas y políticas públicas, en El Colegio de México. Dicha publicación recopila los artículos derivados del seminario del mismo nombre, que se realizó dos años antes, y cuya finalidad fue analizar el problema de la diversidad familiar y su relación con las políticas públicas en México desde un punto de vista académico, manteniendo un marco de pluralidad y ...

  11. Estudio experimental del efecto Magnus en cuerpos cilíndricos de secciones transversales diversas

    OpenAIRE

    Pezzotti, Santiago

    2016-01-01

    El objeto de la presente tesis es el estudio experimental del efecto Magnus en diferentes cuerpos cilíndricos de secciones transversales diversas. Surge del estudio de los antecedentes que el efecto Magnus es un fenómeno aerodinámico de gran importancia, y que ha sido estudiado desde distintas perspectivas, desde su aprovechamiento para propulsión de vehículos, dispositivos hipersustentadores en alas de aeronaves, proyectiles balísticos, deceleradores aerodinámicos, deportes donde s...

  12. Biochemical characteristics of Enterobacter agglomerans and related strains found in buckwheat seeds.

    Science.gov (United States)

    Iimura, K; Hosono, A

    1996-07-01

    Thirty strains of bacteria were randomly isolated and identified from buckwheat seeds. The phenotypic characteristics of these strains agree well with those of the Enterobacter agglomerans-Erwinia herbicola complex. On the basis of the difference in indole production and gas production from D-glucose, the isolates were divided into 3 phenotypic groups, viz. I, II and III. Twenty two strains were in phenotypic group 1, which is negative for indole production and gas production from D-glucose, and resembles Pantoea agglomerans. All six strains in phenotypic group II, which is positive for indole production and negative for gas production from D-glucose, were identified as Erwinia ananas. Two strains in phenotypic group III, which is negative for indole production and positive for gas production from D-glucose, were identified as Rahnella aquatilis.

  13. [Ecological behavior of plasmids and resistance to lead of Enterobacter agglomerans isolated from soil].

    Science.gov (United States)

    Watarai, M; Takeda, K; Uesiba, H

    1991-03-01

    Soil samples taken monthly in 1990 from five parks outside Tokyo were examined for Enterobacter agglomerans. A total of 348 strains were isolated and 250 of them were tested for the presence of plasmids by DNA agarose gel electrophoresis. All the isolates carried at least two kinds of plasmids. Those isolated from July to September showed four or five kinds of plasmids (group I) and those isolated from January to June and from October to December showed two or three kinds of plasmids (group II). A majority of the plasmids detected were of 1,500 or fewer base pairs. The isolates were tested for the Pb2+ resistance; group I strains were more resistant to lead than group II strains. It is presumed that bacterial plasmids are related with the ecosystem of soil and the resistance to lead in E. agglomerans.

  14. Stress tolerance and biocontrol performance of the yeast antagonist, Candida diversa, change with morphology transition.

    Science.gov (United States)

    Li, Guangkun; Chi, Mengshan; Chen, Huizhen; Sui, Yuan; Li, Yan; Liu, Yongsheng; Zhang, Xiaojing; Sun, Zhiqiang; Liu, Guoqing; Wang, Qi; Liu, Jia

    2016-02-01

    As an eco-friendly management method, biological control of postharvest diseases, utilizing antagonistic yeasts, is a research topic receiving considerable attention. Detailed knowledge on the biology of yeast antagonists is crucial when considering their potential application and development as biocontrol products. Changes in the growth form, such as single-cell to pseudohyphae, have been associated with the mode of action in postharvest biocontrol yeasts. In this study, the antagonistic yeast, Candida diversa, reversibly shifted from a single-cell morphology on yeast peptone dextrose (YPD) medium with 2 % agar to a pseudohyphal morphology on YPD with 0.3 % agar. The tolerance of the pseudohyphal form to heat and oxidative stresses, as well as the biocontrol efficacy against Botrytis cinerea on apple and kiwifruit stored at 25 and 4 °C, was significantly higher as compared to the single-cell form. This study provides new information on the ability of C. diversa to change its morphology and the impact of the morphology shift on stress tolerance and biocontrol performance.

  15. Heavy Metal Stress and Its Consequences on Exopolysaccharide (EPS)-Producing Pantoea agglomerans.

    Science.gov (United States)

    Mohite, Bhavana V; Koli, Sunil H; Patil, Satish V

    2018-03-19

    Currently, the heavy metal pollution is of grave concern, and the part of microorganism for metal bioremediation should take into account as an efficient and economic strategy. On this framework, the heavy metal stress consequences on exopolysaccharide (EPS)-producing agricultural isolate, Pantoea agglomerans, were studied. The EPS production is a protective response to stress to survive and grow in the metal-contaminated environment. P. agglomerans show tolerance and mucoid growth in the presence of heavy metals, i.e., mercury, copper, silver, arsenic, lead, chromium, and cadmium. EDX first confirmed the metal accumulation and further, FTIR determined the functional groups involved in metal binding. The ICP-AES identified the location of cell-bound and intracellular metal accumulation. Metal deposition on cell surface has released more Ca 2+ . The effect on bacterial morphology investigated with SEM and TEM revealed the sites of metal accumulation, as well as possible structural changes. Each heavy metal caused distinct change and accumulated on cell-bound EPS with some intracellular deposits. The metal stress caused a decrease in total protein content and increased in total carbohydrate with a boost in EPS. Thus, the performance of P. agglomerans under metal stress indicated a potential candidate for metal bioremediation. Graphical Abstract ᅟ.

  16. Decolorization of azo dyes with Enterobacter agglomerans immobilized in different supports by using fluidized bed bioreactor.

    Science.gov (United States)

    Moutaouakkil, Adnane; Zeroual, Youssef; Dzayri, Fatima Zohra; Talbi, Mohamed; Lee, Kangmin; Blaghen, Mohamed

    2004-02-01

    Immobilized cells of Enterobacter agglomerans, able to reduce azo dyes enzymatically, were used as a biocatalyst for the decolorization of synthetic medium containing the toxic azo dye methyl red (MR). This bacterial strain exhibits high ability to completely decolorize 100 mg/L of MR after only 6 h of incubation under aerobic conditions. Cells of E. agglomerans were immobilized in calcium alginate, polyacylamide, cooper beech, and vermiculite, and were used for the decolorization of MR from synthetic water by using a fluidized bed bioreactor. The highest specific decolorization rate was obtained when E. agglomerans was entrapped in calcium alginate beads and was of about 3.04 mg MR/g cell/h with a 50% conversion time ( t(1/2)) of about 1.6 h. Moreover, immobilized cells in calcium alginate continuously decolorized MR even after seven repeated experiments without significant loss of activity, while polyacrylamide-, cooper beech-, and vermiculite-immobilized cells retained only 62, 15, and 13% of their original activity, respectively.

  17. AHL-type quorum sensing and its regulation on symplasmata formation in Pantoea agglomerans YS19.

    Science.gov (United States)

    Jiang, Jing; Wu, Suisui; Wang, Jieru; Feng, Yongjun

    2015-05-01

    Pantoea agglomerans YS19, an endophytic diazotrophic bacterium isolated from rice, is characterized by the formation of multicellular aggregate structure called symplasmata, which not only bestow the strong stress-resistance of the bacterium, but also contribute to the specific adaptation in the endophyte-host association. Acyl-homoserine lactones (AHLs), as the important signal molecule in the quorum sensing (QS) system of gram-negative bacteria, were demonstrated to regulate motility, cell-aggregation, and other bacterial behaviors. Here, the production of AHL by P. agglomerans YS19 and its regulation on the symplasmata formation were studied. It was revealed that the production of AHL by YS19 was initiated at the exponential growth stage and from then on, reached the peak values at the stationary growth stage in LB medium. The AHL was identified as N-3-oxooctanoyl-L-homoserine lactone (OOHL) by MALDI-TOF-MS analysis. The AHL synthesis gene pagI and receptor gene pagR in YS19 were cloned and phylogenetic analysis showed that they were high conservative among strains in species of P. agglomerans. It was revealed that AHL promoted the bacterial growth and symplasmata formation of YS19. Meanwhile, the colonization ability and growth-promoting effect of YS19 on the host plant were also enhanced by AHL. These results strongly suggest the pleiotropic effects of the AHL-type QS system in endophytic life of the strain. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Optimization of medium for indole-3-acetic acid production using Pantoea agglomerans strain PVM.

    Science.gov (United States)

    Apine, O A; Jadhav, J P

    2011-05-01

    To optimize the medium components for the production of indole-3-acetic acid (IAA) by isolated bacterium Pantoea agglomerans strain PVM. Present study deals with the production of an essential plant hormone IAA by a bacterial isolate P. agglomerans strain PVM identified by 16S rRNA gene sequence analysis. The medium containing 8g l(-1) of meat extract and 1g l(-1) of l-tryptophan (precursor) at optimum pH 7, 30°C and 48-h incubation gave the maximum production of IAA (2·191 g l(-1) ). Effect of IAA synthesized on in vitro root induction in Nicotiana tobacum (leaf) explants was compared with that of control. IAA was characterized by high-performance thin-layer chromatography, high-performance liquid chromatography and gas chromatography-mass spectroscopy. Pantoea agglomerans strain PVM was a good candidate for the inexpensive and utmost production of IAA in short period, as it requires simple medium (meat extract and l-tryptophan). The present report first time showed the rapid, cost-effective and maximum production of IAA. No reports are available on the optimization of particular medium components for the production of IAA. This study demonstrates a novel approach for in vitro root induction in N. tobacum (leaf) explants. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.

  19. Pantoea agglomerans infection behaving like a tumor after plant thorn injury: an unusual presentation.

    Science.gov (United States)

    Jain, Sarika; Bohra, Ishwar; Mahajan, Rakesh; Jain, Sonal; Chugh, T D

    2012-01-01

    Pantoea agglomerans infections in humans are uncommon. Most common infections reported are septic arthritis or synovitis. We report the case of a 25-year-old, healthy male, who presented with indurated swelling over the posterolateral aspect of his right thigh, associated with pain for one month. Magnetic resonance imaging (MRI) revealed muscle edema with cystic areas in the posterior-most part of the vastus lateralis of the right thigh. The condition was clinically diagnosed as a right-sided benign tumor of the vastus lateralis muscle. However, Pantoea agglomerans was isolated on a culture of the excised muscle tissue. On the basis of the awareness of the common association of Pantoea with penetrating trauma by vegetation, the patient was asked to recollect any prior such injury. He then gave a history of a fall in the field and a plant thorn prick in the thigh four years back, when he was an agricultural worker. We emphasize the importance of Pantoea agglomerans infection of the soft tissues that can have an atypical presentation as a non-suppurative, indurated, muscle cyst in our case. Thorn injuries are usually ignored as trivial incidents, however, Pantoea infections should always be borne in mind when encountering soft tissue lesions, as antibiotic treatment is required for complete resolution of the lesion.

  20. Endophthalmitis caused by Pantoea agglomerans: clinical features, antibiotic sensitivities, and outcomes

    Directory of Open Access Journals (Sweden)

    Venincasa VD

    2015-07-01

    Full Text Available Vincent D Venincasa, Ajay E Kuriyan, Harry W Flynn Jr, Jayanth Sridhar, Darlene Miller Department of Ophthalmology, Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, FL, USA Purpose: To report the clinical findings, antibiotic sensitivities, and visual outcomes associated with endophthalmitis caused by Pantoea agglomerans.Methods: A consecutive case series of patients with vitreous culture-positive endophthalmitis caused by P. agglomerans from January 1, 1990 to December 31, 2012 at a large university referral center. Findings from the current study were compared to prior published studies.Results: Of the three study patients that were identified, clinical settings included trauma (n=2 and post-cataract surgery (n=1. Presenting visual acuity was hand motion or worse in all three cases. All isolates were sensitive to ceftazidime, gentamicin, imipenem, and fluoroquinolones. All isolates were resistant to ampicillin. Initial treatment strategies were vitreous tap and intravitreal antibiotic injection (n=1 and pars plana vitrectomy with intravitreal antibiotic injection (n=2. At last follow-up, one patient had no light perception vision, while the other two had best-corrected visual acuity of 20/200 and 20/400.Conclusion: All Pantoea isolates were sensitive to ceftazidime, gentamicin, imipenem, and fluoroquinolones. All patients in the current study received at least one intravitreal antibiotic to which P. agglomerans was shown to be sensitive in vitro. In spite of this, the visual outcomes were generally poor.Keywords: ocular infection, trauma, antibiotic resistance

  1. Endophthalmitis caused by Pantoea agglomerans: clinical features, antibiotic sensitivities, and outcomes

    Science.gov (United States)

    Venincasa, Vincent D; Kuriyan, Ajay E; Flynn, Harry W; Sridhar, Jayanth; Miller, Darlene

    2015-01-01

    Purpose To report the clinical findings, antibiotic sensitivities, and visual outcomes associated with endophthalmitis caused by Pantoea agglomerans. Methods A consecutive case series of patients with vitreous culture-positive endophthalmitis caused by P. agglomerans from January 1, 1990 to December 31, 2012 at a large university referral center. Findings from the current study were compared to prior published studies. Results Of the three study patients that were identified, clinical settings included trauma (n=2) and post-cataract surgery (n=1). Presenting visual acuity was hand motion or worse in all three cases. All isolates were sensitive to ceftazidime, gentamicin, imipenem, and fluoroquinolones. All isolates were resistant to ampicillin. Initial treatment strategies were vitreous tap and intravitreal antibiotic injection (n=1) and pars plana vitrectomy with intravitreal antibiotic injection (n=2). At last follow-up, one patient had no light perception vision, while the other two had best-corrected visual acuity of 20/200 and 20/400. Conclusion All Pantoea isolates were sensitive to ceftazidime, gentamicin, imipenem, and fluoroquinolones. All patients in the current study received at least one intravitreal antibiotic to which P. agglomerans was shown to be sensitive in vitro. In spite of this, the visual outcomes were generally poor. PMID:26185411

  2. Absence of lysogeny in wild populations of Erwinia amylovora and Pantoea agglomerans.

    Science.gov (United States)

    Roach, Dwayne R; Sjaarda, David R; Sjaarda, Calvin P; Ayala, Carlos Juarez; Howcroft, Brittany; Castle, Alan J; Svircev, Antonet M

    2015-05-01

    Lytic bacteriophages are in development as biological control agents for the prevention of fire blight disease caused by Erwinia amylovora. Temperate phages should be excluded as biologicals since lysogeny produces the dual risks of host resistance to phage attack and the transduction of virulence determinants between bacteria. The extent of lysogeny was estimated in wild populations of E. amylovora and Pantoea agglomerans with real-time polymerase chain reaction primers developed to detect E. amylovora phages belonging to the Myoviridae and Podoviridae families. Pantoea agglomerans, an orchard epiphyte, is easily infected by Erwinia spp. phages, and it serves as a carrier in the development of the phage-mediated biological control agent. Screening of 161 E. amylovora isolates from 16 distinct geographical areas in North America, Europe, North Africa and New Zealand and 82 P. agglomerans isolates from southern Ontario, Canada showed that none possessed prophage. Unstable phage resistant clones or lysogens were produced under laboratory conditions. Additionally, a stable lysogen was recovered from infection of bacterial isolate Ea110R with Podoviridae phage ΦEa35-20. These laboratory observations suggested that while lysogeny is possible in E. amylovora, it is rare or absent in natural populations, and there is a minimal risk associated with lysogenic conversion and transduction by Erwinia spp. phages. © 2015 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

  3. Pantoea agglomerans infection behaving like a tumor after plant thorn injury: An unusual presentation

    Directory of Open Access Journals (Sweden)

    Sarika Jain

    2012-01-01

    Full Text Available Pantoea agglomerans infections in humans are uncommon. Most common infections reported are septic arthritis or synovitis. We report the case of a 25-year-old, healthy male, who presented with indurated swelling over the posterolateral aspect of his right thigh, associated with pain for one month. Magnetic resonance imaging (MRI revealed muscle edema with cystic areas in the posterior-most part of the vastus lateralis of the right thigh. The condition was clinically diagnosed as a right-sided benign tumor of the vastus lateralis muscle. However, Pantoea agglomerans was isolated on a culture of the excised muscle tissue. On the basis of the awareness of the common association of Pantoea with penetrating trauma by vegetation, the patient was asked to recollect any prior such injury. He then gave a history of a fall in the field and a plant thorn prick in the thigh four years back, when he was an agricultural worker. We emphasize the importance of Pantoea agglomerans infection of the soft tissues that can have an atypical presentation as a non-suppurative, indurated, muscle cyst in our case. Thorn injuries are usually ignored as trivial incidents, however, Pantoea infections should always be borne in mind when encountering soft tissue lesions, as antibiotic treatment is required for complete resolution of the lesion.

  4. Dichromatic and monochromatic laser radiation effects on survival and morphology of Pantoea agglomerans

    Science.gov (United States)

    Thomé, A. M. C.; Souza, B. P.; Mendes, J. P. M.; Soares, L. C.; Trajano, E. T. L.; Fonseca, A. S.

    2017-05-01

    Despite the beneficial effects of low-level lasers on wound healing, their application for treatment of infected injuries is controversial because low-level lasers could stimulate bacterial growth exacerbating the infectious process. Thus, the aim of this work was to evaluate in vitro effects of low-level lasers on survival, morphology and cell aggregation of Pantoea agglomerans. P. agglomerans samples were isolated from human pressure injuries and cultures were exposed to low-level monochromatic and simultaneous dichromatic laser radiation to study the survival, cell aggregation, filamentation and morphology of bacterial cells in exponential and stationary growth phases. Fluence, wavelength and emission mode were those used in therapeutic protocols for wound healing. Data show no changes in morphology and cell aggregation, but dichromatic laser radiation decreased bacterial survival in exponential growth phase and monochromatic red and infrared lasers increased bacterial survival at the same fluence. Simultaneous dichromatic laser radiation induces biological effects that differ from those induced by monochromatic laser radiation and simultaneous dichromatic laser could be the option for treatment of infected pressure injuries by Pantoea agglomerans.

  5. Familias en el siglo xxi: realidades diversas y políticas públicas

    Directory of Open Access Journals (Sweden)

    Karla Flores Celis

    2012-01-01

    Full Text Available En este texto se sintetizan las intervenciones que se realizaron durante la presentación del libro Familias en el siglo xxi: realidades diversas y políticas públicas, en El Colegio de México. Dicha publicación recopila los artículos derivados del seminario del mismo nombre, que se realizó dos años antes, y cuya finalidad fue analizar el problema de la diversidad familiar y su relación con las políticas públicas en México desde un punto de vista académico, manteniendo un marco de pluralidad y de diálogo con distintos actores de la sociedad.

  6. Factores de riesgo asociados al sexting como umbral de diversas formas de victimización. Estudio de factores correlacionados con el sexting en una muestra universitaria

    Directory of Open Access Journals (Sweden)

    Jose R. Agustina

    2016-07-01

    Full Text Available

    En los últimos años, la irrupción del fenómeno del <em>sexting> ha generado una significativa preocupación mediática y social. La práctica del <em>sexting> se ha revelado problemática, en tanto que puede producir graves consecuencias psicológicas y jurídicas, especialmente en adolescentes. La aparición de tales consecuencias ha puesto de manifiesto la urgente necesidad de desarrollar estrategias de prevención adecuadas. Más aún, mediante el envío de mensajes, imágenes o vídeos de <em>sexting>, menores (y también adultos cruzan inadvertidamente un umbral de riesgo que los expone <em>sin vuelta atrásem> a diversas formas de victimización (chantajes, venganzas o simples indiscreciones altamente perjudiciales. Asimismo, puede suponer el inicio de delitos sexuales iniciados a través de las TIC (Wolak <em>et al.,em> 2004.

     

    En un primer momento, la mayoría de las investigaciones realizadas se centraron en analizar la prevalencia del fenómeno y los motivos que decían tener los encuestados para obrar de este modo. A pesar de las diferencias en los diversos estudios —en función del tipo de muestra y de los parámetros para definir las conductas objeto de cuantificación—, las investigaciones realizadas apuntan sin duda hacia la presencia global de un fenómeno similar. Sin embargo, la investigación apenas ha iniciado la exploración de posibles factores asociados al <em>sexting>. El presente estudio pretende —más allá de medir la prevalencia del fenómeno o describir los motivos aparentes de los encuestados— contrastar algunas hipótesis sobre factores asociados al <em>sexting> basándose en una muestra de 149 estudiantes universitarios en Barcelona (España. Los resultados obtenidos muestran una tasa relativamente alta de comportamientos de <em>sexting> en jóvenes-adultos y sugieren una relación positiva significativa con los siguientes factores: bajos estándares de

  7. DETERMINACIÓN DEL CRECIMIENTO DE HONGOS NEMATÓFAGOS EN DIVERSAS FUENTES DE CARBONO

    Directory of Open Access Journals (Sweden)

    Martha Orozco A.

    2015-01-01

    Full Text Available Las enmiendas orgánicas han sido utilizadas para estimular la acción antagónica de los hongos nematófagos depredadores (HND del suelo, sin embargo, su uso ha producido resultados inconsistentes en el control de nematodos parásitos. Las inconsistencias han sido atribuidas parcialmente a la composición de las enmiendas orgánicas, específicamente al contenido de carbono y nitrógeno. Conocer las preferencias de fuentes de carbono podría ser de utilidad para favorecer la fase depredadora de los HND del suelo. El presente trabajo tiene por objetivo determinar el crecimiento de HND nativos de Costa Rica en fuentes de carbono diversas. Para esto, cepas de HND de las especies Candelabrella musiformis y Arthrobotrys oligospora fueron cultivadas en medios artificiales con diversas fuentes de carbono. La velocidad de crecimiento desarrollada por los HND en los medios de cultivo fue determinada y comparada. Las cepas de C. musiformis y de A. oligospora desarrollaron velocidades de crecimiento en las fuentes de carbono en el siguiente orden: celulosa>quitina> pectina>almidón>leche descremada. Únicamente hubo diferencias significativas en la velocidad de crecimiento en el medio con celulosa, en comparación con los otros medios de cultivo. En dicho medio ambas especies fúngicas crecieron más rápidamente, pero las cepas de A. oligospora crecieron más rápidamente en comparación con las cepas de C. musiformis. Ambas especies de HND desarrollaron velocidades de crecimiento bajas en los medios que contenían almidón y leche descremada.

  8. Phytohormone Production by Strains of Pantoea agglomerans from Knots on Olive Plants Caused by Pseudomonas savastanoi pv. savastanoi

    Directory of Open Access Journals (Sweden)

    A. Cimmino

    2006-12-01

    Full Text Available Pantoea agglomerans is a common epiphyte of many plant species, and it is associated with Pseudomonas savastanoi pv. savastanoi in young and apparently intact olive knots. Strains of P. agglomerans collected from various olive groves in central Italy were studied for their ability to accumulate plant growth substances in culture. All the strains produced indole-3-aldehyde, indole-3-ethanol and indole-3-acetic acid (IAA, this last compound in amounts (average 8.7 mg l-1 comparable to those produced in vitro by virulent strains of P. savastanoi. None of the olive strains produced cytokinins. It is suggested that the IAA produced by P. agglomerans may increase the size of the knots caused on olive by P. savastanoi.

  9. Consecutive episodes of peritonitis in a patient undergoing peritoneal dialysis caused by unusual organisms: Brevibacterium and Pantoea agglomerans

    Directory of Open Access Journals (Sweden)

    Joon Seok Choi

    2012-06-01

    Full Text Available A 52-year-old man undergoing continuous ambulatory peritoneal dialysis presented with two consecutive episodes of peritonitis caused by unusual organisms, namely, Brevibacterium and Pantoea agglomerans. The patient was successfully treated with a 2-week course of cefazolin and ceftazidime for the Brevibacterium-associated peritonitis, and a 3-week course of gentamicin for the P. agglomerans-associated peritonitis. Although these environmental organisms are rarely responsible for human infection, the number of reported cases of human infection by these unusual organisms has increased. This report emphasizes the potential for infection by environmental organisms in patients undergoing peritoneal dialysis.

  10. The purB gene controls rhizosphere colonization by Pantoea agglomerans.

    Science.gov (United States)

    Chauhan, P S; Nautiyal, C S

    2010-02-01

    To isolate the rhizosphere competence-defective transposon Tn5 mutant of Pantoea agglomerans NBRISRM (SRM) and to identify the gene causing defect in its root colonization ability. From over 5000 clones containing Tn5, one mutant P. agglomerans NBRISRMT (SRMT) showing 6 log units less colonization when compared with SRM, after 30 days in sand-nonsterilized soil assay system was selected for further work to determine the effects of the mutation on rhizosphere competence. Southern hybridization analysis of restricted genomic DNA of SRMT demonstrated that the mutant had a single Tn5 insert. SRM increased in titre to about 2 x 10(8) CFU g(-1) root, compared with the indigenous bacterial population of heterotrophs of about 5 x 10(7) CFU g(-1) root. In contrast, 30 days later, the titre value of SRMT was almost undetectable at 1 x 10(2) CFU g(-1) root, demonstrating its inability to survive and colonize the rhizosphere. Sequencing of the flanking region of the Tn5 mutant revealed that Tn5 disrupted the purB gene. A defect in the colonization phenotype of the SRMT was attributed to the disruption in adenylosuccinate lyase (EC 4.3.2.2) which is encoded by the pur B gene and is required for rhizosphere colonization in P. agglomerans. Significantly less exopolysaccharide and biofilm was formed by SRMT when compared to SRM, because of the disruption of the purB gene. This work provides the first evidence for a functional role of purB gene in rhizosphere competence and root colonization by any rhizobacteria.

  11. Isolation and characterization of plant growth promoting traits of a rhizobacteria: Pantoea agglomerans lma2.

    Science.gov (United States)

    Silini-Chérif, H; Silini, A; Ghoul, M; Yadav, S

    2012-03-15

    The use of microbial technology in agriculture is expanding quickly with the identification of new bacterial strains which are more effective in promoting the growth of plants. The rhizobacteria that promote the growth of plants can have a positive effect on the productivity of crops especially when subjected to salt stress. A nitrogen-fixing bacterium was isolated from the wheat rhizosphere of an arid region. The strain was identified on the basis of tests API20E and 16S rRNA sequencing, as Pantoea agglomerans lma2. This strain degraded several carbon sources: sugars (fructose, ribose, dextrin, salicin...), lipids (lecithin, tributyrin and tween 80), proteins (gelatin, casein), grew on KCN and could grow from pH 4 to 8 and had an optimum at pH 7. The growth temperature showed a maximum at 30 degrees C and the bacteria could tolerate from 4 to 41 degrees C and the growth rate was higher when the NaCl concentration was between 100 and 300 mM. The performance of activities enhancing the growth of plants of P. agglomerans lma2 was significantly better in the presence of salt. Rates of Indole Acetic Acid (IAA), siderophores production and solubilization of phosphate increased between 100 and 400 mM NaCl compared to the control without salt. The maximum values were saved to 300 mM for the production of siderophores (18.32%) and solubilization of phosphate (1061.49 microg mL(-1)) and 100 mM for the production of IAA (161 microg mL(-1)). A significant correlation existed between these three activities. These results showed that P. agglomerans lma2 with its Plant Growth Promoting Rhizobacteria (PGPR) and halophilic properties could constitute a good fertilizer in arid and saline zone.

  12. Outbreak of Pantoea agglomerans Bloodstream Infections at an Oncology Clinic-Illinois, 2012-2013.

    Science.gov (United States)

    Yablon, Brian R; Dantes, Raymund; Tsai, Victoria; Lim, Rachel; Moulton-Meissner, Heather; Arduino, Matthew; Jensen, Bette; Patel, Megan Toth; Vernon, Michael O; Grant-Greene, Yoran; Christiansen, Demian; Conover, Craig; Kallen, Alexander; Guh, Alice Y

    2017-03-01

    OBJECTIVE To determine the source of a healthcare-associated outbreak of Pantoea agglomerans bloodstream infections. DESIGN Epidemiologic investigation of the outbreak. SETTING Oncology clinic (clinic A). METHODS Cases were defined as Pantoea isolation from blood or catheter tip cultures of clinic A patients during July 2012-May 2013. Clinic A medical charts and laboratory records were reviewed; infection prevention practices and the facility's water system were evaluated. Environmental samples were collected for culture. Clinical and environmental P. agglomerans isolates were compared using pulsed-field gel electrophoresis. RESULTS Twelve cases were identified; median (range) age was 65 (41-78) years. All patients had malignant tumors and had received infusions at clinic A. Deficiencies in parenteral medication preparation and handling were identified (eg, placing infusates near sinks with potential for splash-back contamination). Facility inspection revealed substantial dead-end water piping and inadequate chlorine residual in tap water from multiple sinks, including the pharmacy clean room sink. P. agglomerans was isolated from composite surface swabs of 7 sinks and an ice machine; the pharmacy clean room sink isolate was indistinguishable by pulsed-field gel electrophoresis from 7 of 9 available patient isolates. CONCLUSIONS Exposure of locally prepared infusates to a contaminated pharmacy sink caused the outbreak. Improvements in parenteral medication preparation, including moving chemotherapy preparation offsite, along with terminal sink cleaning and water system remediation ended the outbreak. Greater awareness of recommended medication preparation and handling practices as well as further efforts to better define the contribution of contaminated sinks and plumbing deficiencies to healthcare-associated infections are needed. Infect Control Hosp Epidemiol 2017;38:314-319.

  13. CONTRIBUIÇÕES DO MARKETING AO GESTOR PÚBLICO PARA O DESENVOLVIMENTO DO TURISMO EM SUAS DIVERSAS FORMAS

    Directory of Open Access Journals (Sweden)

    Deosir Flávio Lobo de Castro Jr

    2013-06-01

    Full Text Available Dentre os desafios encontrados pelos gestores públicos, a geração de emprego e renda para sua região, é um problema corriqueiro. Apresentando-se com um dos maiores percalços, está à falta de conhecimento de como realiza-lo. No presente ensaio teórico verifica-se tipos de turismo e as ferramentas de marketing que poderão contribuir a gestão pública.

  14. Clinical strains of Enterobacter agglomerans (synonyms: Erwinia herbicola, Erwinia milletiae) identified by DNA-DNA-hybridization.

    Science.gov (United States)

    Lind, E; Ursing, J

    1986-08-01

    Using DNA-DNA-hybridization it could be shown that 52 of 86 clinical isolates of Enterobacter agglomerans were closely related to each other, to the type strain of the species and also to the type strains of Erwinia herbicola and Erwinia milletiae. Most of the strains investigated were of the biogroups 1 and G1 of Ewing & Fife. All strains of the genetically defined group belonged to these two biogroups; none of these isolates fermented dulcitol, and with few exceptions they were also cellobiose, lactose and sorbitol negative.

  15. [Application of gas chromatography in the identification of Enterobacter cloacae, Enterobacter aerogenes, and Enterobacter agglomerans].

    Science.gov (United States)

    Robles Valderrama, E; Ramírez García, P; González Arreaga, M E; Sáinz Morales, M G; Martínez Rodríguez, B; Durán Díaz, A; Chávez Ramírez, D

    1999-01-01

    Enterobacter cloacae, Enterobacter aerogenes and Enterobacter agglomerans were identified using gas chromatography as a substitution of the traditional techniques. Their acid methyl esters profiles were determined using a gas chromatograph Hewlett Packard 5890A and a RSL-150 heliflex capillary column. A total of 120 samples were analyzed from reference strains (ATCC 13047, 13048, 27155) and environmental isolations, eleven fatty acids were included in the profiles from which cis-9, 10-methyleneoctadecanoic acid (peak 24), cis-9-hexadecenoic acid (peak 14), octadecanoic acid (peak 23) and dodecanoic acid (peak 3), were the most important for the differentiation of the three species analyzed.

  16. Detection and quantification by PCR assay of the biocontrol agent Pantoea agglomerans CPA-2 on apples.

    Science.gov (United States)

    Soto-Muñoz, Lourdes; Teixidó, Neus; Usall, Josep; Viñas, Inmaculada; Torres, Rosario

    2014-04-03

    The registration of biological control agents requires the development of monitoring systems to detect and quantify the agent in the environment. Pantoea agglomerans CPA-2 is an effective biocontrol agent for postharvest diseases of citrus and pome fruits. The monitoring of CPA-2 in postharvest semi-commercial trials was evaluated by Rodac impression plates and the colonies isolated were confirmed by conventional PCR using the SCAR primers PAGA1 and PAGB1. Samples were taken from different surfaces that had contact with CPA-2, the surrounding environment and working clothes worn by handlers. Moreover, population dynamics of the strain CPA-2 were determined on apple surfaces using both the classical plating technique and real-time quantitative PCR (qPCR). A qPCR assay using a 3'-minor groove-binding (MGB) probe was developed for the specific detection and quantification of P. agglomerans strain CPA-2. Based on the nucleotide sequence of a SCAR fragment of CPA-2, one primer set and TaqMan MGB probe were designed. The primers SP2-F/SP2-R and the TaqMan MGB probe showed a specific detection of strain CPA-2 on apple surfaces, which was verified tested against purified DNA from 17 strains of P. agglomerans, 4 related Pantoea species, and 21 bacterial strains from other genera isolated from whole and also freshly-cut fruit and vegetables. The detection level was approximately 10(3) cells per reaction, and the standard curve was linear within a range of 5log units. Results from semi-commercial trials showed that CPA-2 had a low impact. The maximum persistence of P. agglomerans CPA-2 was not longer than 5days in plastic boxes stored at 0°C. Significant differences in CPA-2 population level dynamics were observed in results obtained by qPCR and dilution plating. These differences may indicate the presence of non-degraded DNA from non-viable cells. In conclusion, qPCR is a novel potential tool to quickly and specifically monitor recent surface colonisation by CPA-2

  17. Fermentative hydrogen production by the new marine Pantoea agglomerans isolated from the mangrove sludge

    Energy Technology Data Exchange (ETDEWEB)

    Zhu, Daling [College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457 (China); Wang, Guangce [College of Marine Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457 (China); Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 (China); Qiao, Hongjin; Cai, Jinling [Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 (China)

    2008-11-15

    A new fermentative hydrogen-producing bacterium was isolated from mangrove sludge and identified as Pantoea agglomerans using light microscopic examination, biological tests and 16S rRNA gene sequence analysis. The isolated bacterium, designated as P. agglomerans BH-18, is a new strain that has never been optimized as a potential hydrogen-producing bacterium. In this study, the culture conditions and the hydrogen-producing ability of P. agglomerans BH-18 were examined. The strain was a salt-tolerant facultative anaerobe with the initial optimum pH value at 8.0-9.0 and temperature at 30 C on cell growth. During fermentation, hydrogen started to evolve when cell growth entered late-exponential phase and was mainly produced in the stationary phase. The strain was able to produce hydrogen over a wide range of initial pH from 5 to 10, with an optimum initial pH of 6. The level of hydrogen production was affected by the initial glucose concentration, and the optimum value was found to be 10 g glucose/l. The maximum hydrogen-producing yield (2246 ml/l) and overall hydrogen production rate (160 ml/l/h) were obtained at an initial glucose concentration of 10 g/l and an initial pH value of 7.2 in marine culture conditions. In particular, the level of hydrogen production was also affected by the salt concentration. Hydrogen production reached a higher level in fresh culture conditions than in marine ones. In marine conditions, hydrogen productivity was 108 ml/l/h at an initial glucose concentration of 20 g/l and pH value of 7.2, whereas, it increased by 27% in fresh conditions. In addition, this strain could produce hydrogen using glucose and many other carbon sources such as fructose, sucrose, sorbitol and so on. As a result, it is possible that P. agglomerans BH-18 is used for biohydrogen production and biological treatment of mariculture wastewater and marine organic waste. (author)

  18. Characterization of the O-antigen polysaccharide derived from Pantoea agglomerans IG1 lipopolysaccharide.

    Science.gov (United States)

    Hashimoto, Masahito; Satou, Rune; Ozono, Mami; Inagawa, Hiroyuki; Soma, Gen-Ichiro

    2017-09-08

    A polysaccharide fraction was isolated from the Pantoea agglomerans IG1 lipopolysaccharide (IP-PA1), and its O-antigenic polysaccharide was characterized by chemical analyses and 1D and 2D 1 H and 13 C NMR spectroscopy. The polysaccharide is composed of linear tetrasaccharide repeating units, consisting of glucose and rhamnose, where 40% of one of the rhamnose residues is substituted with glucose: →2)-α-l-Rhap-(1→6)-α-d-Glcp-(1→2)-[β-d-Glcp-(1→3)] 0.4 -α-l-Rhap-(1→2)-α-l-Rhap-(1→. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. New Blue Pigment Produced by Pantoea agglomerans and Its Production Characteristics at Various Temperatures ▿

    OpenAIRE

    Fujikawa, Hiroshi; Akimoto, Ryo

    2010-01-01

    A bacterium capable of producing a deep blue pigment was isolated from the environment and identified as Pantoea agglomerans. The pigment production characteristics of the bacterium under various conditions were studied. The optimal agar plate ingredients for pigment production by the bacterium were first studied: the optimal ingredients were 5 g/liter glucose, 10 g/liter tryptic soy broth, and 40 g/liter glycerol at pH 6.4. Bacterial cells grew on the agar plate during the incubation, while ...

  20. Sob o signo do Acordos de Oslo: perspectivas diversas sobre o conflito israelo-palestino

    Directory of Open Access Journals (Sweden)

    Marcos Chor MAIO

    2014-12-01

    Full Text Available Este artigo está dividido em duas partes: 1ª discorre sobre a relevância dos Acordos de Oslo para o processo de paz entre israelenses e palestinos nos anos 1990; 2ª aborda brevemente um conjunto de estudos históricos, sociológicos e políticos que versam sobre as relações entre Israel e Palestina em diferentes níveis, incluindo-se o papel da diplomacia brasileira neste domínio.

  1. A visão de especialistas sobre a sustentabilidade corporativa frente às diversas formações de cadeias produtivas

    Directory of Open Access Journals (Sweden)

    Rafael Fey Jappur

    2008-10-01

    Full Text Available Para que as organizações sejam sustentáveis, estas devem buscar um equilíbrio entre as três dimensões que balizam o conceito de sustentabilidade corporativa: a econômica, a ambiental e a social. Porém, estes princípios são muitas vezes vistos como conflitantes, perante a busca por resultados financeiros imediatos, aumento de fatias de mercado e competitividade, sobretudo no setor industrial, objeto de estudo deste trabalho. A globalização e a mudança em torno da competitividade nos últimos anos mudaram a lógica da gestão estratégica empresarial, deixando de estar focalizada nas próprias organizações e voltando-se para as relações interempresariais, através das diversas cadeias produtivas que as formam. Considerando estas premissas, o presente trabalho analisou a percepção especializada, através da utilização do método Delphi, da aplicabilidade dos métodos de sustentabilidade corporativa frente às diversas formações de cadeias produtivas. Dentre outros resultados, pôde-se constatar que a competitividade de um negócio está vinculada às relações interempresariais da cadeia produtiva, independente do tipo de cadeia. Sendo que a sustentabilidade corporativa, dentro desta perspectiva, reverte-se num importante fator crítico de sucesso, visto que permeia a viabilidade econômica, ambiental e social de qualquer tipo de negócio.

  2. Evidence that antibiotic of Pantoea agglomerans E325 is produced and active against Erwinia amylovora on stigmas of pomaceous blossoms

    Science.gov (United States)

    Pantoea agglomerans strain E325, the active ingredient in a commercial product for fire blight, previously was shown to produce a unique pH-sensitive inhibitor in vitro that is specific to E. amylovora. To evaluate antibiosis as a mode of antagonism of E325, Tn5 mutagenesis was used to generate...

  3. Development of species-, strain- and antibiotic biosynthesis-specific quantitative PCR assays for Pantoea agglomerans as tools for biocontrol monitoring.

    Science.gov (United States)

    Braun-Kiewnick, Andrea; Lehmann, Andreas; Rezzonico, Fabio; Wend, Chris; Smits, Theo H M; Duffy, Brion

    2012-09-01

    Pantoea agglomerans is a cosmopolitan plant epiphytic bacterium that includes some of the most effective biological antagonists against the fire blight pathogen Erwinia amylovora, a major threat to pome fruit production worldwide. Strain E325 is commercially available as Bloomtime Biological™ in the USA and Canada. New quantitative PCR (qPCR) assays were developed for species- and strain -specific detection in the environment, and for detection of indigenous strains carrying the biocontrol antibacterial peptide biosynthesis gene paaA. The qPCR assays were highly specific, efficient and sensitive, detecting fewer than three cells per reaction or 700 colony forming units per flower, respectively. The qPCR assays were tested on field samples, giving first indications to the incidence of P. agglomerans E325 related strains, total P. agglomerans and pantocin A producing bacteria in commercial orchards. These assays will facilitate monitoring the environmental behavior of biocontrol P. agglomerans after orchard application for disease protection, proprietary strain-tracking, and streamlined screening for discovery of new biocontrol strains. Copyright © 2012 Elsevier B.V. All rights reserved.

  4. Potential of osmoadaptation for improving Pantoea agglomerans E325 as biocontrol agent for fire blight of apple and pear

    Science.gov (United States)

    Pantoea agglomerans biocontrol strain E325 is the active ingredient in a commercial product for fire blight, a destructive disease of apple and pear initiated by Erwinia amylovora in flowers. Osmoadaptation, involving the combination of saline osmotic stress and osmolyte amendment to growth media, w...

  5. Identification of Candida tropicalis BH-6 and synergistic effect with Pantoea agglomerans BH-18 on hydrogen production in marine culture.

    Science.gov (United States)

    Zhu, Daling; Ma, Yingchao; Wang, Guangce; Pan, Guanghua

    2015-03-01

    A marine yeast was isolated from mangrove sludge and named Candida tropicalis BH-6. The optimum temperature and the initial pH value for growth of the isolated strain were 37 °C and 5.0, respectively. The strain had high salt tolerance and could survive at NaCl concentrations of 0-6 %. Additionally, the yield of hydrogen production by C. tropicalis BH-6 was only 66.30 ml/l. However, when the yeast was mixed with Pantoea agglomerans BH-18, hydrogen production increased significantly to a maximum of 1707.5 ml/l, which was 36.94 and 247.54 % higher than the monoculture of P. agglomerans BH-18 and C. tropicalis BH-6, respectively. Taken together, these results revealed that in mixed culture, the yeast strain isolated from the same ecosystem as P. agglomerans BH-18 likely consumed the organic acids produced by fermentation, thus eliminating the factor inhibiting hydrogen production by P. agglomerans BH-18. As a result, the yield of hydrogen production during mixed culture increased significantly.

  6. EFECTO DE DIVERSAS ACTIVIDADES FÍSICAS Y DEPORTIVAS SOBRE LA AUTOEFICACIA DE ESTUDIANTES UNIVERSITARIOS COSTARRICENSES

    Directory of Open Access Journals (Sweden)

    Cinthya Campos Salazar

    2013-01-01

    Full Text Available El presente artículo fue realizado con estudiantes universitarios matriculados en el curso de “Actividad Deportiva” de la Universidad de Costa Rica. Los propósitos fueron: (1 comprobar si existe relación significativa entre el nivel de autoeficacia, entendida como la confianza en sí mismo para iniciar y mantenerse en un plan de ejercicio, y la cantidad de actividad física que reporta el estudiantado al inicio del curso; (2 establecer si existe un efecto significativo de las diversas actividades físicas y deportivas en el nivel de autoeficacia, tanto para efectuar ejercicio como para no abandonarlo; y (3 comprobar si los cambios en la autoeficacia son influenciados por el género o el tipo de actividad física realizada (cursos de acondicionamiento físico versus cursos de deportes. Se trabajó con 211 estudiantes matriculados en el III ciclo de 2009, durante el verano. Al estudiantado se le aplicó, antes de iniciar el curso y una vez finalizado, la encuesta de autoeficacia para comportamiento de ejercicio y el Cuestionario Internacional de Actividad Física (IPAQ, con el fin de medir el nivel de actividad física. Los resultados mostraron una asociación positiva entre el nivel de actividad física reportada por el(la estudiante y el nivel de autoeficacia (p<0.001. En cuanto a este último, las mujeres presentaron niveles inferiores que los varones (p<0.001, asimismo el nivel de autoeficacia aumentó significativamente (p<0.05 independientemente del género y del tipo de actividad física. En conclusión, los cursos de actividad deportiva produjeron efectos positivos en la autoeficacia de los participantes, además, los estudiantes más activos presentaron mayores niveles de ella.

  7. Pantoea agglomerans: a mysterious bacterium of evil and good. Part III. Deleterious effects: infections of humans, animals and plants

    Directory of Open Access Journals (Sweden)

    Jacek Dutkiewicz

    2016-06-01

    Full Text Available [i]Pantoea agglomerans[/i], a bacterium associated with plants, is not an obligate infectious agent in humans. However, it could be a cause of opportunistic human infections, mostly by wound infection with plant material, or as a hospital-acquired infection, mostly in immunocompromised individuals. Wound infection with [i]P. agglomerans[/i] usually follow piercing or laceration of skin with a plant thorn, wooden splinter or other plant material and subsequent inoculation of the plant-residing bacteria, mostly during performing of agricultural occupations and gardening, or children playing. Septic arthritis or synovitis appears as a common clinical outcome of exogenous infection with [i]P. agglomerans[/i], others include endophthalmitis, periostitis, endocarditis and osteomyelitis. Another major reason for clinical infection with [i]P. agglomerans[/i] is exposure of hospitalized, often immunodeficient individuals to medical equipment or fluids contaminated with this bacterium. Epidemics of nosocomial septicemia with fatal cases have been described in several countries, both in adult and paediatric patients. In most cases, however, the clinical course of the hospital-acquired disease was mild and application of the proper antibiotic treatment led to full recovery. Compared to humans, there are only few reports on infectious diseases caused by [i]Pantoea agglomerans[/i] in vertebrate animals. This species has been identified as a possible cause of equine abortion and placentitis and a haemorrhagic disease in dolphin fish ([i]Coryphaena hippurus[/i]. [i]P. agglomerans[/i] strains occur commonly, usually as symbionts, in insects and other arthropods. [i]Pantoea agglomerans[/i] usually occurs in plants as an epi- or endophytic symbiont, often as mutualist. Nevertheless, this species has also also been identified as a cause of diseases in a range of cultivable plants, such as cotton, sweet onion, rice, maize, sorghum, bamboo, walnut, an ornamental

  8. Isolation of endophytic diazotroph Pantoea agglomerans and nondiazotroph Enterobacter asburiae from sweetpotato stem in Japan.

    Science.gov (United States)

    Asis, C A; Adachi, K

    2004-01-01

    To isolate and identify diazotrophic endophytes in the stem of Japanese sweetpotato cv. Koganesengan. Surface-sterilized and thinly sliced (1-2 mm) sweetpotato stem samples were incubated in test tubes with semi-solid modified Rennie (MR) medium. The test tubes were assayed for acetylene reduction activity (ARA) 5 days after incubation at 30 degrees C. Twelve isolates were obtained from MR plates inoculated with a loop of semi-solid MR medium from ARA+ tubes. However, ARA test showed that only nine isolates were diazotrophic and three were nondiazotrophic strains. Using the API 20E diagnostic kit, four diazotrophic isolates were identified as strains of Pantoea spp. and five isolates as Klebsiella spp. The nondiazotrophic bacteria were strains of Enterobacter spp. A diazotrophic isolate Pantoea sp. MY1 and nondiazotrophic isolate Enterobacter sp. MY2 were identified to the species level by full sequence analysis of 16S rRNA gene. The results showed that MY1 had 99.2% similarity to Pantoea agglomerans ATCC 27155 and MY2 had 99.5% similarity to Enterobacter asburiae ATCC 35953. The stem of sweetpotato cv. Koganesengan was colonized by diazotrophic endophyte P. agglomerans and nondiazotrophic endophyte E. asburiae. This study is an essential step toward understanding the ecology and interaction between endophytic bacteria and sweetpotato.

  9. 252Cf plasma-desorption mass spectrometry of lipid A from Enterobacter agglomerans.

    Science.gov (United States)

    Cole, R B; Domelsmith, L N; David, C M; Laine, R A; DeLucca, A J

    1992-10-01

    Endotoxins from gram-negative bacteria are believed to be causative agents of byssinosis, an occupational pulmonary disease associated with exposure to cotton dust in textile mills. Lipid A preparations from Enterobacter agglomerans, a gram-negative bacterium commonly found in cotton and cotton dust, have been analyzed using plasma-desorption mass spectrometry. Results indicate the existence of at least two lipid A types which differ only by the presence of an additional oxygen atom whose position has been localized to the acyloxyacyl ester-linked side-chain of the distal portion of the molecule. The lower molecular weight compound of the two structures has the same molecular weight and presumably the same empirical formula as a well-characterized lipid A from Salmonella minnesota. The mass spectra of lipid A compounds obtained from S. minnesota and E. agglomerans show strong similarities. Palmitoyl, hydroxymyristoyl, myristoyl, and lauroyl side-chains which are known to be present in the former are inferred from spectral evidence to be present in the latter.

  10. Molecular cloning, structural analysis, and expression in Escherichia coli of a chitinase gene from Enterobacter agglomerans.

    Science.gov (United States)

    Chernin, L S; De la Fuente, L; Sobolev, V; Haran, S; Vorgias, C E; Oppenheim, A B; Chet, I

    1997-03-01

    The gene chiA, which codes for endochitinase, was cloned from a soilborne Enterobacter agglomerans. Its complete sequence was determined, and the deduced amino acid sequence of the enzyme designated Chia_Entag yielded an open reading frame coding for 562 amino acids of a 61-kDa precursor protein with a putative leader peptide at its N terminus. The nucleotide and polypeptide sequences of Chia_Entag showed 86.8 and 87.7% identity with the corresponding gene and enzyme, Chia_Serma, of Serratia marcescens, respectively. Homology modeling of Chia_Entag's three-dimensional structure demonstrated that most amino acid substitutions are at solvent-accessible sites. Escherichia coli JM109 carrying the E. agglomerans chiA gene produced and secreted Chia_Entag. The antifungal activity of the secreted endochitinase was demonstrated in vitro by inhibition of Fusarium oxysporum spore germination. The transformed strain inhibited Rhizoctonia solani growth on plates and the root rot disease caused by this fungus in cotton seedlings under greenhouse conditions.

  11. Electrospray mass spectrometry for characterization of lipid A from Enterobacter agglomerans.

    Science.gov (United States)

    Harrata, A K; Domelsmith, L N; Cole, R B

    1993-01-01

    Negative ion and positive ion electrospray mass spectrometry have been employed to characterize the lipid A mixture produced by hydrolysis of lipopolysaccharides from Enterobacter agglomerans, a Gram-negative bacterium commonly found in field cotton. Neutral monophosphoryl lipid A molecules form stable anions via deprotonation, but adduct formation via cation attachment occurs in low yield. Dephosphorylated lipid A molecules, on the other hand, readily form adducts with Na+, whereas deprotonation occurs in low yield. The mass spectra of lipid A produced by E. agglomerans reveal the presence of lipid A ions which differ in the nature of attached fatty acid side chains. At least two heptaacyl forms of lipid A are present, one of which has a structure which appears to be the same as the structure of heptaacyl lipid A produced by Salmonella minnesota. The second structure differs only by the nature of the side chain at position 3' of the disaccharide backbone where a hydroxymyristoyloxymyristoyl group replaces the myristoyloxymyristoyl substituent. Collisionally activated dissociations prior to mass analysis enable the identification of fragment ions which can be distinguished from at least eight intact deprotonated molecules present in crude lipid A.

  12. Bacteriophages LIMElight and LIMEzero of Pantoea agglomerans, belonging to the "phiKMV-like viruses".

    Science.gov (United States)

    Adriaenssens, Evelien M; Ceyssens, Pieter-Jan; Dunon, Vincent; Ackermann, Hans-Wolfgang; Van Vaerenbergh, Johan; Maes, Martine; De Proft, Maurice; Lavigne, Rob

    2011-05-01

    Pantoea agglomerans is a common soil bacterium used in the biocontrol of fungi and bacteria but is also an opportunistic human pathogen. It has been described extensively in this context, but knowledge of bacteriophages infecting this species is limited. Bacteriophages LIMEzero and LIMElight of P. agglomerans are lytic phages, isolated from soil samples, belonging to the Podoviridae and are the first Pantoea phages of this family to be described. The double-stranded DNA (dsDNA) genomes (43,032 bp and 44,546 bp, respectively) encode 57 and 55 open reading frames (ORFs). Based on the presence of an RNA polymerase in their genomes and their overall genome architecture, these phages should be classified in the subfamily of the Autographivirinae, within the genus of the "phiKMV-like viruses." Phylogenetic analysis of all the sequenced members of the Autographivirinae supports the classification of phages LIMElight and LIMEzero as members of the "phiKMV-like viruses" and corroborates the subdivision into the different genera. These data expand the knowledge of Pantoea phages and illustrate the wide host diversity of phages within the "phiKMV-like viruses."

  13. The cytidine repressor participates in the regulatory pathway of indole in Pantoea agglomerans.

    Science.gov (United States)

    Jia, Mengqi; Yu, Xuemei; Jiang, Jing; Li, Zihua; Feng, Yongjun

    2017-09-01

    Indole, an important signal molecule in both intraspecies and interspecies, regulates a variety of bacterial behaviors, but its regulatory mechanism is still unknown. Pantoea agglomerans YS19, a preponderant endophytic bacterium isolated from rice, does not produce indole, yet it senses exogenous indole. In this study, a mutant of YS19-Rp r whose target gene expression was downregulated by indole was selected through mTn5 transposon mutagenesis. Using the TAIL-PCR technique, the mutation gene was identified as a cytR homologue, which encodes a cytidine repressor (CytR) protein, a bacterial transcription factor involved in a complex regulation scheme. The negative regulation of indole in cytR, which is equivalent to the mutation in cytR, promotes the expression of a downstream gene deoC, which encodes the key enzyme deoxyribose-phosphate aldolase in participating in pentose metabolism. We found that DeoC is one of the regulatory proteins of P. agglomerans that is involved in counteracting starvation. Furthermore, the expression of deoC was induced by starvation conditions, accompanied by a decrease in cytR expression. This finding suggests that the indole signal and the mutation of cytR relieve inhibition of CytR in the transcription of deoC, facilitating better adaptation of the bacterium to the adverse conditions of the environment. Copyright © 2017 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

  14. Clinical Effects of Orally Administered Lipopolysaccharide Derived from Pantoea agglomerans on Malignant Tumors.

    Science.gov (United States)

    Morishima, Atsutomo; Inagawa, Hiroyuki

    2016-07-01

    It has been reported that oral administration of lipopolysaccharide (LPS) recovers an individual's immune condition and induces the exclusion of foreign matter, inflammation and tissue repair. We orally administered LPS from the wheat symbiotic bacteria Pantoea agglomerans, which has been ingested and proven to be safe, to cancer patients. Our observation of clinical improvements resulting from this treatment are reported. Sixteen cancer patients who exhibited declined small intestinal immune competence were treated between June and September, 2015. Diagnosis was based on our evaluation on small intestinal immune competence and macrophage activity. The state of malignant tumors at 3 months after starting this treatment was complete recovery for 3 cases, remission for 7 cases, maintenance for 4 cases, exacerbation for 1 case and death for 1 case (total response rate=62.5%). Small intestinal immune competence and macrophage activity recovered in all cases, suggesting that oral administration of LPS contributes to disease improvement. No clear side-effects that appeared to be related to LPS intake were noted. Intake of an appropriate level of Pantoea agglomerans LPS recovers small intestinal immune competence and macrophage activity, contributing to improvement of malignant tumors' therapy. Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  15. [A case of ventilator-associated pneumonia due to Pantoea agglomerans].

    Science.gov (United States)

    Kurşun, Ozlem; Unal, Nesrin; Cesur, Salih; Altın, Nilgün; Canbakan, Başol; Argun, Cem; Koldaş, Kamer; Sencan, Irfan

    2012-04-01

    Pantoea species, which are the members of Enterobacteriaceae family are gram-negative bacilli that are frequently found on many plants and in soil. They may lead to localized infections in healthy subjects and systemic infections in immunosuppressed patients. In this case report a ventilator-associated pneumoniae due to Pantoea agglomerans was presented. A 55 year-old male patient with chronic renal failure was hospitalized in intensive care unit following cardiopulmonary arrest. The patient developed fever (38.8°C), had pulmonary infiltrations in chest X-ray and leucocytosis. Treatment with piperacillintazobactam was initiated upon diagnosis of nosocomial pneumoniae. Bacterial growth from the deep tracheal aspirate of the patient was identified as P.agglomerans by VITEK2 automated system (bioMérieux, France) and the identification was confirmed by conventional microbiological methods. Since the strain was susceptible to the cephoperazon-sulbactam, tobramycin, tetracycline, gentamicin and levofloxacine, the treatment was changed to levofloxacine and cephoperazon-sulbactam and the patient improved. This case was presented to withdraw attention to rare opportunistic pathogens that may lead to nosocomial infections particularly in patients with underlying diseases.

  16. Lipopolysaccharides of Pantoea agglomerans 7604 and 8674 with structurally related O-polysaccharide chains: Chemical identification and biological properties.

    Science.gov (United States)

    Zdorovenko, Evelina L; Kadykova, Alexandra A; Shashkov, Alexander S; Varbanets, Ludmila D; Bulyhina, Tetiana V; Knirel, Yuriy A

    2018-02-01

    Structurally related O-specific polysaccharide (O-antigen) and lipid A components were obtained by mild acid degradation of the lipopolysaccharides (LPSs) of two strains of bacteria Pantoea agglomerans, 7604 and 8674. Studies by sugar analysis along with 1D and 2D 1 H and 13 C NMR spectroscopy enabled elucidation of the following structures of the O-polysaccharides, which differ only in the linkage configuration of a side-chain glucose residue: R=α-d-Glcp in strain 7604 or β-d-Glcp in strain 8674 Lipid A samples were studied by GC-MS and high-resolution ESI-MS and found to be represented by penta- and tetra-acyl species; lipid A of strain 8674 also included hexaacyl species. A peculiar feature of lipid A of both strains is the presence of the major cis-9-hexadecenoic (palmitoleic) acid, which has not been found in P. agglomerans strains studied earlier. The LPSs of both strains were pyrogenic, reduced the average adhesion and the index of adhesiveness and showed a relatively low level of lethal toxicity. O-antiserum against strain 7604 showed one-way cross-reactivity with the LPS of strain 8674, and O-antisera against both strains cross-reacted with LPSs of some other Р. agglomerans strains but more strains were serologically unrelated. These structural and serological data indicate immunochemical heterogeneity of Р. agglomerans strains and will find demand in classification of Р. agglomerans by O-antigens. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Pantoea agglomerans pneumonia in a heart-lung transplant recipient: case report and a review of an emerging pathogen in immunocompromised hosts.

    Science.gov (United States)

    Shubov, A; Jagannathan, P; Chin-Hong, P V

    2011-10-01

    Pantoea agglomerans is a gram-negative rod that is frequently found on the exterior of many plants, fruits, vegetables, and in soil, and it is used as a biopesticide in the agriculture industry. Recent reports have implicated P. agglomerans in systemic infections of immunocompromised hosts and neonates, as well as more localized infections in healthy hosts. P. agglomerans as a cause of hospital-acquired pneumonia has not been well characterized. We report a case of P. agglomerans pneumonia in a heart-lung transplant recipient following transplantation. The organism was susceptible to multiple antimicrobial agents and treated successfully with ertapenem. We review the patient's course and the relevant literature, and discuss implications for the future. © 2011 John Wiley & Sons A/S.

  18. Orar, ensinar ou agir. As diversas formas de cumprir o Carisma de Sion

    Directory of Open Access Journals (Sweden)

    Angela Xavier de Brito

    Full Text Available Resumo Fundada pelo Pe Ratisbonne em 1842 para converter os judeus, o carisma de Sion oscila entre a oração, o ensino e a ação direta. Sion foi mais conhecida por seus internatos até os anos 1960, quando a incorporação das Ancelles (1937 e a virada ecumênica a fizeram retornar a suas origens. A pesquisa histórica realizada nos arquivos de Paris mostra que as tarefas educativas levaram as Irmãs a negligenciar o carisma original até o Concílio Vaticano II (1962-65, quando confiaram seus colégios a leigas, saíram para se formar em teologia e estudos bíblicos e participar do movimento de estima mútua com relação ao povo judeu.

  19. Optimization of Culture Medium Enhances Viable Biomass Production and Biocontrol Efficacy of the Antagonistic Yeast, Candida diversa

    Directory of Open Access Journals (Sweden)

    Jia Liu

    2017-10-01

    Full Text Available Viable biomass production is a key determinant of suitability of antagonistic yeasts as potential biocontrol agents. This study investigated the effects of three metal ions (magnesium, ferrous, and zinc on biomass production and viability of the antagonistic yeast, Candida diversa. Using response surface methodology to optimize medium components, a maximum biomass was obtained, when the collective Mg2+, Fe2+, and Zn2+ concentrations were adjusted in a minimal mineral (MM medium. Compared with the unmodified MM, and three ion-deficient MM media, yeast cells cultured in the three ion-modified MM medium exhibited a lower level of cellular oxidative damage, and a higher level of antioxidant enzyme activity. A biocontrol assay indicated that C. diversa grown in the ion-modified MM exhibited the greatest level of control of gray mold on apple fruit. These results provide new information on culture medium optimization to grow yeast antagonists in order to improve biomass production and biocontrol efficacy.

  20. Estudo comparativo do desempenho óptico de diversas lentes intra-oculares (LIO) multifocais

    OpenAIRE

    Antunes, Telma Nicole Nogueira

    2012-01-01

    A cirurgia da catarata tem vindo a evoluir ao longo dos anos, tendo sido realizado o primeiro implante de uma lente intra-ocular em 1949. Desde então, várias técnicas foram utilizadas e descontinuadas devido a insucessos. Actualmente é utilizada a técnica de facoemulsificação. Tal como a técnica cirúrgica a lente implantada também tem vindo a evoluir, tanto a nível do material como a nível de design. Com as evoluções, tanto a nível de técnica cirúrgica como a nível de forma e material das len...

  1. [Labelling of nif-plasmid pEA9 from Enterobacter agglomerans 339].

    Science.gov (United States)

    Liu, Cheng-jun; Klingmüller, Walter

    2002-07-01

    The authors describe the in vivo labelling of the plasmid pEA9 in Enterobacter agglomerans 339 with a kanamycin resistance gene. For labelling purposes the donor plasmid pST5 was constructed. This plasmid contains the nif ENX region from pEA9,in which a kanamycin resistance gene is cloned.pST5 was transformed into E.a.339 and subsequently cured from the host. Curing was achieved with AP medium. Fourty strains that had lost pST5,but retained the kanamycin resistance, could be isolated. It showed that none of these clones contained co-integrates of pST5 and pEA9. This is evident that in all clones the kanamycin resistance gene was integrated into pEA9 by homologous recombination.

  2. IS1222: analysis and distribution of a new insertion sequence in Enterobacter agglomerans 339.

    Science.gov (United States)

    Steibl, H D; Lewecke, F M

    1995-04-14

    With a length of 1221 bp and 44-bp inverted repeats with ten mismatches, IS1222 was identified as an endogenous insertion sequence in Enterobacter agglomerans 339. In this host strain, four copies were located, three on the nif plasmid pEA9 and one at the chromosome. Sequence analysis showed two consecutive open reading frames, orfA and orfB, encoding putative polypeptides of 87 and 276 amino acids. In-between both reading frames, a potential frameshift window of the homonucleotide type was postulated, followed by a pseudoknot structure and a ribosome-binding site. Based on significant homology at the sequence level and similarity of the features discussed, IS1222 was placed among the group of IS3 elements with IS407, IS476 and ISR1 being the most closely related IS. Hybridization experiments suggest that the distribution of IS1222 is limited to a group of related bacterial strains among Enterobacteriaceae.

  3. ECOLOGY OF PANTOEA AGGLOMERANS 2066-7 STRAIN: A BIOLOGICAL CONTROL OF BACTERIA ONION DISEASES

    Directory of Open Access Journals (Sweden)

    Soumia Sadik

    2016-06-01

    Full Text Available The growth response of the biocontrol agent Pantoea agglomerans 2066-7 to change in water activity (aw, temperature, and pH was determined in vitro in basic medium. The minimum temperature at which 2066-7 was able to grow was 7°C, and the growth of 2066-7 did not change at varying pH levels (4–10.34. The best growth was obtained at a water activity of 0.98 in all media modified with the four solutes (glucose, glycerol, NaCl and polyethylene glycol. The solute used to reduce water activity had a great influence on bacterial growth, especially at unfavorable conditions (low temperature. This study has defined the range of environmental conditions (aw, pH, and temperature over which the bacteria may be developed for biological control of plant diseases.

  4. An unusual cause of peritonitis in peritoneal dialysis patients: Pantoea agglomerans.

    Science.gov (United States)

    Kazancioglu, Rumeyza; Buyukaydin, Banu; Iraz, Meryem; Alay, Murat; Erkoc, Reha

    2014-07-14

    Peritonitis is a serious infection and early diagnosis and treatment is mandatory. A variety of microorganisms are identified in these cases and during recent years a new one was included, Pantoea agglomerans. In this case report, a female patient on continuous ambulatory peritoneal dialysis therapy with a peritonitis episode caused by this organism is described. The source of infection was thought to be due to contact of catheter with non-sterile surfaces. In microbiologic culture, this organism was identified and the patient successfully treated with a three week course of gentamicin therapy. The number of reported cases with this organism has increased in last years and various infection localizations and clinical progress patterns have been identified. In peritoneal dialysis patients presenting with peritonitis, this organism must be kept in mind.

  5. Dissimilatory Metal Reduction by the Facultative Anaerobe Pantoea agglomerans SP1

    Science.gov (United States)

    Francis, Chris A.; Obraztsova, Anna Y.; Tebo, Bradley M.

    2000-01-01

    Anaerobic enrichments with acetate as the electron donor and Fe(III) as the terminal electron acceptor were obtained from sediments of Salt Pond, a coastal marine basin near Woods Hole, Mass. A pure culture of a facultatively anaerobic Fe(III) reducer was isolated, and 16S rRNA analysis demonstrated that this organism was most closely related to Pantoea (formerly Enterobacter) agglomerans, a member of the family Enterobacteriaceae within the gamma subdivision of the Proteobacteria. This organism, designated strain SP1, can grow by coupling the oxidation of acetate or H2 to the reduction of a variety of electron acceptors, including Fe(III), Mn(IV), Cr(VI), and the humic substance analog 2,6-anthraquinone disulfonate, but not sulfate. To our knowledge, this is the first mesophilic facultative anaerobe reported to couple acetate oxidation to dissimilatory metal reduction. PMID:10653716

  6. Poly(A) polymerase I participates in the indole regulatory pathway of Pantoea agglomerans YS19.

    Science.gov (United States)

    Li, Zihua; Jiang, Jing; Yu, Xuemei; Wu, Cunxiang; Shen, Delong; Feng, Yongjun

    2017-02-01

    Pantoea agglomerans YS19 is a preponderant endophytic bacterium isolated from rice. It is characterized by the formation of symplasmata, a type of multicellular aggregate structure, contributing to a strong stress resistance and specific adaptation of YS19 in endophyte-host associations. Indole is an important signal molecule in intra- or interspecies relationships, regulating a variety of bacterial behaviours such as cell aggregation and stress resistance; however, the regulatory mechanism remains an ongoing area of investigation. This study selected YS19 as a model strain to construct a mutant library, utilizing the mTn5 transposon mutagenesis method, thus obtaining a positive mutant with an indole-inhibited mutation gene. Via thermal asymmetric interlaced PCR, the mutational site was identified as the gene of pcnB, which encodes the poly(A) polymerase I to catalyse the polyadenylation of RNAs. The full length of the pcnB sequence was 1332 bp, and phylogenetic analysis revealed that pcnB is extremely conserved among strains of P. agglomerans. The expression of the gene was significantly inhibited (by 36.6 % as detected via quantitative PCR) by indole (0.5 mM). Many physiological behaviours of YS19 were affected by this mutation: the cell decay rate in the post-stationary growth phase was promoted, symplasmata formation and motility were inhibited in the late stationary growth phase and the colonization ability and growth-promoting effect of YS19 on the host plant were also inhibited. This study discusses the indole regulatory pathways from the point of RNA post-transcriptional modification, thus enriching our knowledge of polyadenylation and expanding current research ideas of indole regulation.

  7. New blue pigment produced by Pantoea agglomerans and its production characteristics at various temperatures.

    Science.gov (United States)

    Fujikawa, Hiroshi; Akimoto, Ryo

    2011-01-01

    A bacterium capable of producing a deep blue pigment was isolated from the environment and identified as Pantoea agglomerans. The pigment production characteristics of the bacterium under various conditions were studied. The optimal agar plate ingredients for pigment production by the bacterium were first studied: the optimal ingredients were 5 g/liter glucose, 10 g/liter tryptic soy broth, and 40 g/liter glycerol at pH 6.4. Bacterial cells grew on the agar plate during the incubation, while the pigment spread into the agar plate, meaning that it is water soluble. Pigment production was affected by the initial cell density. Namely, at higher initial cell densities ranging from 10(6.3) to 10(8.2) CFU/cm(2) on the agar plate, faster pigment production was observed, but no blue pigment was produced at a very high initial density of 10(9.1) CFU/cm(2). Thus, the cell population of 10(8.2) CFU/cm(2) was used for subsequent study. Although the bacterium was capable of growing at temperatures above and below 10°C, it could produce the pigment only at temperatures of ≥10°C. Moreover, the pigment production was faster at higher temperatures in the range of 10 to 20°C. Pigment production at various temperature patterns was well described by a new logistic model. These results suggested that the bacterium could be used in the development of a microbial temperature indicator for the low-temperature-storage management of foods and clinical materials. To our knowledge, there is no other P. agglomerans strain capable of producing a blue pigment and the pigment is a new one of microbial origin.

  8. Evaluation of the heteroantagonism between Enterobacter agglomerans strains isolated from vulture (Coragyps atratus for production and Pseudomonas aeruginosa as developed

    Directory of Open Access Journals (Sweden)

    L.A. Lima

    2011-06-01

    Full Text Available The heteroantagonism between Enterobacter agglomerans, isolated from the gastrointestinal tract of American vulture Coragyps atratus, and Pseudomonas aeruginosa isolated from a hospital environment was evaluated. The slow (layer and the wells (direct techniques were tested, using agar and soy tryptone broth pH 7.3 at 37ºC. Through the slow method from 196 tests, inhibition growth halos, related heteroantagonism phenomenon observed in 118, corresponding to 60.2% positive results. Equivalent positive results were detected using wells (direct methodology. The seven samples of E. agglomerans tested were capable of revealing heteroantagonism in the experimental conditions; antagonism reveled by the presence of a clear growth inhibition halo. The added 1% yeast extract to media was adequate for revealing antagonisms best.

  9. Nosocomial outbreak of Pantoea agglomerans bacteraemia associated with contaminated anticoagulant citrate dextrose solution: new name, old bug?

    Science.gov (United States)

    Boszczowski, I; Nóbrega de Almeida Júnior, J; Peixoto de Miranda, E J; Pinheiro Freire, M; Guimarães, T; Chaves, C E; Cais, D P; Strabelli, T M V; Risek, C F; Soares, R E; Rossi, F; Costa, S F; Levin, A S

    2012-03-01

    We describe an outbreak investigation of Pantoea agglomerans bacteraemia associated with anticoagulant citrate-dextrose 46% (ACD) solution prepared in-house. A healthy man presented with septic shock during plasmapheresis for granulocyte donation. The solution used for priming and blood samples were sent for culture. Identification of the isolate to species level was performed by gyrB sequencing. Typing was performed by pulsed-field gel electrophoresis (PFGE). In total, eight cases were identified during a three-week period. P. agglomerans was also cultured from six ACD solution bags. Isolates from patients and ACD bags were identical by PFGE. All isolates were susceptible to ampicillin, cephazolin, gentamicin, ciprofloxacin, cefepime and imipenem. Copyright © 2011 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

  10. Potencial de redução da temperatura de superfície pelo aumento do albedo nas diversas regiões brasileiras

    Directory of Open Access Journals (Sweden)

    Dominique E. Giordano

    2014-08-01

    Full Text Available Com o crescimento urbano, as superfícies vegetais são progressivamente substituídas por materiais construtivos que absorvem mais radiação solar e retém o calor, alterando o microclima das cidades, contribuindo para a intensificação da ilha de calor urbano e causando desconforto térmico em regiões mais quentes e nos períodos de verão, além de um maior consumo de energia para refrigeração das edificações. Uma estratégia para reduzir a temperatura de superfície é a utilização de materiais de superfície com maior albedo. Sendo a temperatura de superfície resultado tanto da energia incidente como do albedo, o objetivo deste trabalho foi o de mapear a Irradiância Global Horizontal (IGH média nas diversas regiões brasileiras de maneira a poder correlacioná-la com o potencial de redução da temperatura superficial que se pode atingir com o incremento do albedo, servindo de orientação para a decisão de onde o uso de um maior albedo pode ser efetivamente útil e quanto de melhora isso pode resultar. A metodologia do trabalho consistiu em calcular a IGH Média a partir da base de dados compilada pelo Prof. Maurício Roriz, a partir de dados horários, registrados em 411 estações climatológicas do INMET entre os anos de 2000 e 2010. O potencial de redução de temperatura foi estimado a partir de equação empírica apresentada na literatura da área. O resultado do trabalho foi convertido em mapa temático, utilizando-se do método de krigagem para interpolação dos dados amostrais. Percebe-se a partir dele uma grande variação no potencial de redução da temperatura superficial, tanto entre regiões distintas, quanto para uma mesma latitude. Palavras-Chave: albedo, microclima urbano, ilhas de calor urbano, conforto térmico, eficiência energética.

  11. Distribution and replication of the pathogenicity plasmid pPATH in diverse populations of the gall-forming bacterium Pantoea agglomerans.

    Science.gov (United States)

    Weinthal, Dan M; Barash, Isaac; Panijel, Mary; Valinsky, Lea; Gaba, Victor; Manulis-Sasson, Shulamit

    2007-12-01

    Pantoea agglomerans has been transformed from a commensal bacterium into two related gall-forming pathovars by acquisition of pPATH plasmids containing a pathogenicity island (PAI). This PAI harbors an hrp/hrc gene cluster, type III effectors, and phytohormone biosynthetic genes. DNA typing by pulsed-field gel electrophoresis revealed two major groups of P. agglomerans pv. gypsophilae and one group of P. agglomerans pv. betae. The pPATH plasmids of the different groups had nearly identical replicons (98% identity), and the RepA protein showed the highest level of similarity with IncN plasmid proteins. A series of plasmids, designated pRAs, in which the whole replicon region (2,170 bp) or deleted derivatives of it were ligated with nptI were generated for replicon analysis. A basic 929-bp replicon (pRA6) was sufficient for replication in Escherichia coli and in nonpathogenic P. agglomerans. However, the whole replicon region (pRA1) was necessary for expulsion of the pPATH plasmid, which resulted in the loss of pathogenicity. The presence of direct repeats in the replicon region suggests that the pPATH plasmid is an iteron plasmid and that the repeats may regulate its replication. The pPATH plasmids are nonconjugative but exhibit a broad host range, as shown by replication of pRA1 in Erwinia, Pseudomonas, and Xanthomonas. Restriction fragment length polymorphism analyses indicated that the PAIs in the two groups of P. agglomerans pv. gypsophilae are similar but different from those in P. agglomerans pv. betae. The results could indicate that the pPATH plasmids evolved from a common ancestral mobilizable plasmid that was transferred into different strains of P. agglomerans.

  12. Cotranscription of the electron transport protein genes nifJ and nifF in Enterobacter agglomerans 333.

    Science.gov (United States)

    Kreutzer, R; Dayananda, S; Klingmüller, W

    1991-05-01

    A nucleotide sequence showing extensive homology to the nifF gene, which codes for a flavodoxin involved in nitrogen fixation in Klebsiella pneumoniae, was localized on the plasmid pEA3 of Enterobacter agglomerans and determined. The analysis of transcriptional fusions, as well as transcript protection assays, indicated a novel nif gene organization, that is, the cotranscription of nifJ and nifF.

  13. Cotranscription of the electron transport protein genes nifJ and nifF in Enterobacter agglomerans 333.

    OpenAIRE

    Kreutzer, R; Dayananda, S; Klingmüller, W

    1991-01-01

    A nucleotide sequence showing extensive homology to the nifF gene, which codes for a flavodoxin involved in nitrogen fixation in Klebsiella pneumoniae, was localized on the plasmid pEA3 of Enterobacter agglomerans and determined. The analysis of transcriptional fusions, as well as transcript protection assays, indicated a novel nif gene organization, that is, the cotranscription of nifJ and nifF.

  14. Enterobacter agglomerans lipopolysaccharide-induced changes in pulmonary surfactant as a factor in the pathogenesis of byssinosis.

    Science.gov (United States)

    DeLucca, A J; Brogden, K A; Engen, R

    1988-04-01

    Lipopolysaccharide (LPS) from Enterobacter agglomerans and pulmonary surfactant mixtures were centrifuged in discontinuous sucrose gradients to determine whether LPS bound to surfactant and examined in a Langmuir trough with a Wilhelmy balance to determine whether LPS altered the surface activity of surfactant. The LPS was found to bind to the surfactant and altered its surface tension properties. The binding of LPS to surfactant in the lung may change the physiological properties of surfactant and be a possible mechanism for the pathogenesis of byssinosis.

  15. Relationship between the physiology of Enterobacter agglomerans CNCM 1210 grown anaerobically on glycerol and the culture conditions.

    Science.gov (United States)

    Barbirato, F; Bories, A

    1997-01-01

    In a preliminary study, levels of activity of enzymes involved in anaerobic glycerol catabolism by Enterobacter agglomerans grown in batch cultures regulated in a pH range of 6.5-8.0 were monitored. That study showed that activities of key enzymes of the downstream metabolism of glycerol--glyceradehyde-3-phosphate dehydrogenase (GAP-DH), lactate dehydrogenase and pyruvate formate lyase--were strongly dependent on the culture pH. To investigate the influence of pH on the physiology of the strain, E. agglomerans was grown anaerobically in a continuous culture supplied with glycerol as the sole carbon source and regulated at pH 8. A complete biochemical analysis was performed and was compared with that previously described for the continuous culture regulated at pH 7. A limitation of the glycolytic flux at the level of GAP-DH was demonstrated at high dilution rate, resulting in an overflow metabolism through the 1,3-propanediol formation pathway. Increasing the specific rate of glycerol consumption also resulted in enhanced lactate production due to limitation by the pyruvate decarboxylation step. Finally, changing the culture pH significantly modified the enzymatic profile of E. agglomerans, and it enabled the stability of the culture to be increased by preventing the accumulation in the fermentation broth of 3-hydroxypropionaldehyde, an inhibitory metabolite, when the glycerol supply was suddenly increased.

  16. pMH2, a small plasmid bearing the nif gene cluster of Enterobacter agglomerans 333 as an excisable cassette.

    Science.gov (United States)

    Stumpf, F; Halda, L; Klingmüller, W

    1993-10-01

    A small plasmid containing the entire nif gene cluster of Enterobacter agglomerans 333 as an excisable cassette has been constructed, using pACYC177 as a vector. Two cosmid clones taken from a gene library of E. agglomerans plasmid pEA3 were used as a source of nif genes. A SmaI fragment of peaMS2-2, containing the H,D,K,Y,E,N,X,U,S,V,W,Z,M,L,A and B genes and an ApaI fragment of peaMS2-16 containing nif A,B,Q,F and J were selected to construct pMH2. The resulting plasmid of 33 kb carries the complete nif gene cluster as a nif cassette on a single XbaI fragment. The nif construct pMH2 in Escherichia coli strains has significant nitrogenase activity compared to wild-type E. agglomerans 333. The nif gene cluster construct was found to be very stable.

  17. Site-specific mutagenesis in Enterobacter agglomerans: construction of nif B mutants and analysis of the gene's structure and function.

    Science.gov (United States)

    Siddavattam, D; Nickles, A; Herterich, S; Steibl, H D; Kreutzer, R; Klingmüller, W

    1995-12-15

    A novel technique was developed which may be generally well suited to the site-specific construction of mutations in Enterobacter agglomerans. The method is based on the observation that E. agglomerans can be cured of a plasmid of the incompatibility group IncQ by cultivation on citrate-containing medium. To test the applicability of this technique, we inserted a kanamycin cassette into the cloned nifB gene, transferred it into E. agglomerans, and selected for recombinants in which the wild-type nifB was replaced by the mutated gene by growing transformants on citrate medium with kanamycin. The nifB- mutants with the kanamycin cassette inserted in either orientation showed a nif- phenotype. Further, we determined the nucleotide sequence of nifB. A typical sigma 54-dependent promoter and a consensus NifA binding site were found upstream of nifB. Activation of this promoter by both heterologous and homologous NifA proteins was observed in vivo. The predicted amino acid sequence of the NifB protein showed strong similarity to the NifB sequences of other diazotrophic bacteria. The typical clustering of cysteine residues at the N-terminal end indicates its involvement in Fe-Mo cofactor biosynthesis.

  18. Pantoea agglomerans as an indicator of a foreign body of plant origin in cases of wound infection.

    Science.gov (United States)

    Vaiman, M; Lazarovich, T; Lotan, G

    2013-04-01

    To investigate the role of Pantoea agglomerans as an infectious agent that causes infection in a wound even after the wound was managed at the emergency department. A retrospective cohort study, reviewing the medical records of patients with traumatic wounds that were admitted to the emergency department from 2007-20 12 and had signs of wound infection for more than I 0 days after the wound was managed. Bacteriological results, clinical picture,and treatment results were obtained. Nine cases were identified. Pantoea agglomerans was detected in all cases. After 1-2 months of ineffective treatment, patients were hospitalised and surgical revisions of the wounds were performed.In all cases, small foreign bodies of plant origin were detected. After surgical revision, wounds were healed in 2-3 days. In cases of prolonged healing of post-traumatic wounds, the presence of foreign bodies of plant origin infected with Pantoea agglomerans should be taken into account. Removal of such foreign bodies leads to rapid healing of the wounds.

  19. 2-Amino-3-(Oxirane-2,3-Dicarboxamido)-Propanoyl-Valine, an Effective Peptide Antibiotic from the Epiphyte Pantoea agglomerans 48b/90 ▿

    Science.gov (United States)

    Sammer, Ulrike F.; Völksch, Beate; Möllmann, Ute; Schmidtke, Michaela; Spiteller, Peter; Spiteller, Michael; Spiteller, Dieter

    2009-01-01

    The epiphyte Pantoea agglomerans 48b/90, which has been isolated from soybean leaves, belongs to the Enterobacteriaceae, as does the plant pathogen Erwinia amylovora, which causes fire blight on rosaceous plants such as apples and leads to severe economic losses. Since P. agglomerans efficiently antagonizes phytopathogenic bacteria, the P. agglomerans strain C9-1 is used as a biocontrol agent (BlightBan C9-1). Here we describe the bioassay-guided isolation of a peptide antibiotic that is highly active against the plant pathogen E. amylovora and pathovars of Pseudomonas syringae, and we elucidate its structure. Bioassay-guided fractionation using anion-exchange chromatography followed by hydrophobic interaction liquid chromatography yielded the bioactive, highly polar antibiotic. The compound was identified as 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine by using high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance techniques. This peptide was found to be produced by three of the nine P. agglomerans strains analyzed. Notably, the biocontrol strain P. agglomerans C9-1 also produces 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine. Previously, 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine has been characterized only from Serratia plymuthica. 2-Amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine has been shown to inhibit the growth of the human pathogen Candida albicans efficiently, but its involvement in the defense of epiphytes against phytopathogenic bacteria has not been investigated so far. PMID:19820144

  20. Isolation, identification and antimicrobial susceptibility of Pantoea (Enterobacter) agglomerans isolated from consumed powdered infant formula milk (PIF) in NICU ward: First report from Iran.

    Science.gov (United States)

    Mardaneh, Jalal; Dallal, Mohammad Mehdi Soltan

    2013-09-01

    Pantoea agglomerans is a Gram-negative rod in the Enterobacteriaceae family. It is reported as both commensal and opportunistic pathogen of animals and humans. This organism is potential candidates as powdered infant milk formula-borne opportunistic pathogen. The aim of our study was to perform isolation, identification and antimicrobial susceptibility pattern of Pantoea (Enterobacter) agglomerans strains isolated from consumed powdered infant formula milk (PIF) in NICU ward. A of total 125 powdered infant formula milk (PIF) samples were purchased from hospital drug stores between June 2011 to March 2012. P. agglomerans was isolated according to FDA method. For final confirmation, biochemical tests embedded in the API-20E system were used. The drug susceptibility test was performed using the disc diffusion method according to CLSI guidelines. Out of the 125 samples investigated, 8 (6.4%) samples were positive for P. agglomerans and these were uniformly susceptible to tigecycline, chloramphenicol, cefepime, levofloxacin, minocycline and colistin. Fifty percent of isolates were resistant to cefotaxime, moxifloxacin, cotrimoxazole and ticarcillin. Controlling the primary populations of P. agglomerans during the PIF production process and preventing post processing contamination, by using suitable microbiological guidelines, is accessible. Sanitary practices for the preparation of infant formula in both the home and hospitals should be carefully controlled.

  1. Acid tolerance response induced in the biocontrol agent Pantoea agglomerans CPA-2 and effect on its survival ability in acidic environments.

    Science.gov (United States)

    Cañamás, T P; Viñas, I; Abadias, M; Usall, J; Torres, R; Teixidó, N

    2009-01-01

    The aim of this work was to optimize acid stress conditions for induction of acid tolerance response (ATR) in the biocontrol agent Pantoea agglomerans and study the effect of ATR induced on the ability to survive under acidic conditions. Initially, Pantoea agglomerans was grown in mild acidic conditions (pH 6.0, 5.5, 5.0 and 4.0) in order to induce ATR. The highest ATR was induced at initial pH of 5 using malic or citric acid. A first in vitro experiment was carried out. Thus, basal liquid medium at different pHs (3.0, 3.5, 4.0 and non-acidified) were then inoculated with acid-adapted and non-adapted inocula of P. agglomerans and survivals were examined during incubation at 25 or 4 degrees C. It was found that acid adaptation enhanced the survivals of Pantoea agglomerans CPA-2 cells at pH levels at which the cells were unable to grow (Pantoea agglomerans to mild acidic conditions could induce acid resistance in this biocontrol agent.

  2. 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine, an effective peptide antibiotic from the epiphyte Pantoea agglomerans 48b/90.

    Science.gov (United States)

    Sammer, Ulrike F; Völksch, Beate; Möllmann, Ute; Schmidtke, Michaela; Spiteller, Peter; Spiteller, Michael; Spiteller, Dieter

    2009-12-01

    The epiphyte Pantoea agglomerans 48b/90, which has been isolated from soybean leaves, belongs to the Enterobacteriaceae, as does the plant pathogen Erwinia amylovora, which causes fire blight on rosaceous plants such as apples and leads to severe economic losses. Since P. agglomerans efficiently antagonizes phytopathogenic bacteria, the P. agglomerans strain C9-1 is used as a biocontrol agent (BlightBan C9-1). Here we describe the bioassay-guided isolation of a peptide antibiotic that is highly active against the plant pathogen E. amylovora and pathovars of Pseudomonas syringae, and we elucidate its structure. Bioassay-guided fractionation using anion-exchange chromatography followed by hydrophobic interaction liquid chromatography yielded the bioactive, highly polar antibiotic. The compound was identified as 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine by using high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance techniques. This peptide was found to be produced by three of the nine P. agglomerans strains analyzed. Notably, the biocontrol strain P. agglomerans C9-1 also produces 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine. Previously, 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine has been characterized only from Serratia plymuthica. 2-Amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine has been shown to inhibit the growth of the human pathogen Candida albicans efficiently, but its involvement in the defense of epiphytes against phytopathogenic bacteria has not been investigated so far.

  3. Transmission of Pantoea ananatis and P. agglomerans, causal agents of center rot of onion (Allium cepa), by onion thrips (Thrips tabaci) through feces.

    Science.gov (United States)

    Dutta, B; Barman, A K; Srinivasan, R; Avci, U; Ullman, D E; Langston, D B; Gitaitis, R D

    2014-08-01

    Frankliniella fusca, the tobacco thrips, has been shown to acquire and transmit Pantoea ananatis, one of the causal agents of the center rot of onion. Although Thrips tabaci, the onion thrips, is a common pest of onions, its role as a vector of P. ananatis has been unknown. The bacterium, P. agglomerans, is also associated with the center rot of onion, but its transmission by thrips has not been previously investigated. In this study, we investigated the relationship of T. tabaci with P. ananatis and P. agglomerans. Surface-sterilized T. tabaci were provided with various acquisition access periods (AAP) on onion leaves inoculated with either P. ananatis or P. agglomerans. A positive exponential relationship was observed between thrips AAP duration and P. ananatis (R² = 0.967; P = 0.023) or P. agglomerans acquisition (R² = 0.958; P = 0.017). Transmission experiments conducted with T. tabaci adults indicated that 70% of the seedlings developed center rot symptoms 15 days after inoculation. Immunofluorescence microscopy with antibodies specific to P. ananatis revealed that the bacterium was localized only in the gut of T. tabaci adults. Mechanical inoculation of onion seedlings with fecal rinsates alone produced center rot but not with salivary secretions. Together these results suggested that T. tabaci could efficiently transmit P. ananatis and P. agglomerans.

  4. La formación docente como estrategia pedagógica para fortalecer la educación diversa

    OpenAIRE

    Guapucal Cuasanchir, María Edith; Jaguandoy Chamorro, Mónica Johana

    2015-01-01

    El artículo, La Formación docente como estrategia pedagógica para fortalecer la educación diversa, tiene como propósito identificar y analizar los procesos de enseñanza-aprendizaje que se llevan a cabo en la Institución Educativa Luis Irizar Salazar del municipio de Barbacoas por parte de los docentes para diferenciar los aprendizajes de los estudiantes de los grados cuarto y quinto de primaria de las áreas de castellano y matemáticas. Para esto se aplican encuestas a los docentes y estudiant...

  5. <em>Angiostrongylus vasorumem> in red foxes (<em>Vulpes vulpesem> and badgers (<em>Meles melesem> from Central and Northern Italy

    Directory of Open Access Journals (Sweden)

    Marta Magi

    2010-06-01

    Full Text Available Abstract During 2004-2005 and 2007-2008, 189 foxes (<em>Vulpes vulpesem> and 6 badgers (<em>Meles melesem> were collected in different areas of Central Northern Italy (Piedmont, Liguria and Tuscany and examined for <em>Angiostrongylus vasorumem> infection. The prevalence of the infection was significantly different in the areas considered, with the highest values in the district of Imperia (80%, Liguria and in Montezemolo (70%, southern Piedmont; the prevalence in Tuscany was 7%. One badger collected in the area of Imperia turned out to be infected, representing the first report of the parasite in this species in Italy. Further studies are needed to evaluate the role played by fox populations as reservoirs of infection and the probability of its spreading to domestic dogs.
    Riassunto <em>Angiostrongylus vasorumem> nella volpe (<em>Vulpes vulpesem> e nel tasso (<em>Meles melesem> in Italia centro-settentrionale. Nel 2004-2005 e 2007-2008, 189 volpi (<em>Vulpes vulpesem> e 6 tassi (<em>Meles melesem> provenienti da differenti aree dell'Italia settentrionale e centrale (Piemonte, Liguria Toscana, sono stati esaminati per la ricerca di <em>Angiostrongylus vasorumem>. La prevalenza del nematode è risultata significativamente diversa nelle varie zone, con valori elevati nelle zone di Imperia (80% e di Montezemolo (70%, provincia di Cuneo; la prevalenza in Toscana è risultata del 7%. Un tasso proveniente dall'area di Imperia è risultato positivo per A. vasorum; questa è la prima segnalazione del parassita in tale specie in Italia. Ulteriori studi sono necessari per valutare il potenziale della volpe come serbatoio e la possibilità di diffusione della parassitosi ai cani domestici.

    doi:10.4404/hystrix-20.2-4442

  6. Physiologic Mechanisms Involved in Accumulation of 3-Hydroxypropionaldehyde during Fermentation of Glycerol by Enterobacter agglomerans.

    Science.gov (United States)

    Barbirato, F; Soucaille, P; Bories, A

    1996-12-01

    When grown in 700 mM glycerol within the pH range 6.0 to 7.5, anaerobic pH-regulated cultures of Enterobacter agglomerans exhibited an extracellular accumulation of 3-hydroxypropionaldehyde (3-HPA). This phenomenon, which causes fermentation cessation, occurred earlier when pH was low. In contrast, substrate consumption was complete at pH 8. Levels of glycerol-catabolizing enzymes, i.e., glycerol dehydrogenase and dihydroxyacetone kinase for the oxidative route and glycerol dehydratase and 1,3-propanediol dehydrogenase for the reductive route, as well as the nucleotide pools were determined periodically in the pH 7- and pH 8-regulated cultures. A NAD/NADH ratio of 1.7 was correlated with the beginning of the production of the inhibitory metabolite. Further accumulation was dependent on the ratio of glycerol dehydratase activity to 1,3-propanediol dehydrogenase activity. For a ratio higher than 1, 3-HPA was produced until fermentation ceased, which occurred for the pH 7-regulated culture. At pH 8, a value below 1 was noticed and 3-HPA accumulation was transient, while the NAD/NADH ratio decreased. The low rate of glycerol dissimilation following the appearance of 3-HPA in the culture medium was attributed to the strong inhibitory effect exerted by 3-HPA on glycerol dehydrogenase activity.

  7. Anaerobic pathways of glycerol dissimilation by Enterobacter agglomerans CNCM 1210: limitations and regulations.

    Science.gov (United States)

    Barbirato, F; Astruc, S; Soucaille, P; Camarasa, C; Salmon, J M; Bories, A

    1997-07-01

    Continuous cultures of Enterobacter agglomerans CNCM 1210 were performed under regulated pH conditions (pH 7.0) with glycerol or glucose (20 g l-1) as carbon source. Cultures grown on glucose produced mainly acetate, ethanol and formate. In contrast, 1,3-propanediol (PPD) was the main product with glycerol. The carbon flow distribution at branching metabolic points was investigated. Higher PPD yields with increased dilution rate were correlated with an important increase in the relative ratio of glycerol dehydratase to glycerol dehydrogenase. Determination of intracellular triose-phosphate and fructose 1,6-biphosphate concentrations demonstrated that glyceraldehyde-3-phosphate dehydrogenase is the limiting step in glycerol dissimilation. At the pyruvate branching point, pyruvate dehydrogenase (PDH) activity was systematically detected. The pyruvate flow shifted to PDH is suspected to represent up to 22% of the acetyl-CoA formed. In addition, this enzyme pattern combined with the enhanced in vivo lactate dehydrogenase activity at high growth rates, was correlated with a decrease in the pyruvate formate-lyase activity. A regulation of this latter enzyme by the accumulation of triose-phosphate is suspected.

  8. Purine metabolizing capability of Enterobacter agglomerans affects volatiles production and attractiveness to Mexican fruit fly.

    Science.gov (United States)

    Robacker, David C; Lauzon, Carol R

    2002-08-01

    We investigated two strains of Enterobacter agglomerans that differ in their ability to metabolize uric acid for (1) attractiveness to sugar-fed Mexican fruit flies, and (2) production of volatile chemicals that may be responsible for the attractiveness. The two strains were cultured on a medium that contained uric acid as the primary nitrogen source to simulate bird feces, a natural substrate for this bacterium. Active cultures of both strains were more attractive than uninoculated uric acid medium to both sexes of sugar-fed flies in wind-tunnel bioassays. The uricase(+) strain was more attractive than the uricase(-) strain to males and to females <9 days old, but not to older females. Volatiles found by solid-phase microextraction in greater amounts in headspace above active cultures of both strains than above uninoculated medium were ammonia, dimethyldisulfide, 3-methylbutanol, 2-phenylethanol, 2,5-dimethylpyrazine, and trimethylpyrazine. The uricase(+) strain produced more ammonia, dimethyldisulfide, and trimethylpyrazine than the uricase(-) strain. An additional chemical, 3-hydroxybutanone, appears to be produced exclusively by the uricase(+) strain. The uricase(-) strain produced more 2-phenylethanol than the uricase(+) strain. Differences in volatiles are consistent with the generally greater attractiveness of the uricase(+) strain compared with the uricase(-) strain as ammonia, 3-hydroxybutanone, and trimethylpyrazine have been demonstrated attractive to sugar-fed Mexican fruit flies.

  9. A novel beta-galactosidase capable of glycosyl transfer from Enterobacter agglomerans B1.

    Science.gov (United States)

    Lu, Lili; Xiao, Min; Xu, Xiaodong; Li, Zhengyi; Li, Yumei

    2007-04-27

    A novel transglycosylating beta-galactosidase was purified from Enterobacter agglomerans B1. It was a homodimer of approximately 248 kDa. The optimal pH and temperature for oNPGal hydrolysis were 7.5-8.0 and 37-40 degrees C, respectively. The K(m) values for oNPGal and lactose were 0.06 and 114 mM, respectively. The enzyme produced galacto-oligosaccharides in a 38% yield at the lactose concentration of 12.5% (w/v). When using oNPGal as donor, the enzyme was able to catalyze glycosyl transfer to a series of acceptors, including hexose, pentose, beta- or alpha-disaccharides, hexahydroxy alcohol, cyclitol, and aromatic glycosides. This suggested the enzyme to be a potential synthetic tool for preparing galactose-containing chemicals. The gene encoding this enzyme was cloned by degenerate PCR and TAIL-PCR. It revealed an ORF of 3090 nucleotides encoding a 1029 amino-acid protein, which had been expressed in Escherichia coli. Transferase activities in both recombinant and natural enzymes were similar.

  10. Controlled release of Pantoea agglomerans E325 for biocontrol of fire blight disease of apple.

    Science.gov (United States)

    Kim, In-Yong; Pusey, Paul Lawrence; Zhao, Youfu; Korban, Schuyler S; Choi, Hyungsoo; Kim, Kyekyoon Kevin

    2012-07-10

    Microencapsulation and controlled release of the biocontrol agent Pantoea agglomerans strain E325 (E325), an antagonist to the bacterial plant pathogen Erwinia amylovora that causes fire blight, a devastating disease of apple and pear, have been investigated. Uniform core-shell alginate microcapsules (AMCs), 60-300 μm in diameter, were fabricated to encapsulate E325 within the core, along with nutrients, to preserve viability and promote proliferation. Controlled release of E325 was achieved by separately adjusting alginate concentrations in the shell and core solutions, and by modifying the AMC size. Viability of E325 was monitored via fluorescent staining, revealing either lack of or minimal stress during or after encapsulation. Proliferation of E325 within AMCs, followed by their subsequent release, and colonization activities within confines of apple flowers were studied under different encapsulation conditions using rfp-labeled E325 to obtain highly promising results. This study provided a 'proof of concept' of the successful use of a microencapsulated biocontrol agent, E325, against E. amylovora, and could serve as a model for further studies on the development of effective plant disease management strategies. Copyright © 2012 Elsevier B.V. All rights reserved.

  11. Primed Activation of Macrophages by Oral Administration of Lipopolysaccharide Derived from Pantoea agglomerans.

    Science.gov (United States)

    Inagawa, Hiroyuki; Kobayashi, Yutaro; Kohchi, Chie; Zhang, Ran; Shibasaki, Yasuhiro; Soma, Gen-Ichiro

    2016-01-01

    Bacterial lipopolysaccharide (LPS) is involved in the activation of the innate immune responses on monocytes/macrophages in vitro, and by intravenous injection. Although small quantities of LPS are usually found in traditional Chinese medicines, vegetables and fruits, the mode of action of orally administered LPS is still unclear. LPS derived from Pantoea agglomerans (LPSp) was orally administered to C3H/HeN or C3H/HeJ mice ad libitum. The LPSp treatment enhanced phagocytosis by resident peritoneal macrophages of C3H/HeN mice but not of C3H/HeJ mice. This activation can be defined as primed activation because no augmentation of inflammatory cytokines production was detected. LPSp in peritoneal fluid was detected and successfully quantified. Moreover, the LPSp reduced the expression of avian reticuloendotheliosis viral oncogene-related B (RelB) in the macrophages without degradation of nuclear factor of kappa light polypeptide gene enhancer in B-cell inhibitor, alpha (IκBα). Orally administered LPSp can reach the peritoneum, and enhance phagocytosis via Toll-like receptor 4 signaling pathway in resident peritoneal macrophages. Copyright © 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  12. Pantoea agglomerans Strain EH318 Produces Two Antibiotics That Inhibit Erwinia amylovora In Vitro

    Science.gov (United States)

    Wright, Sandra A. I.; Zumoff, Cathy H.; Schneider, Lois; Beer, Steven V.

    2001-01-01

    Pantoea agglomerans (synonym: Erwinia herbicola) strain Eh318 produces through antibiosis a complex zone of inhibited growth in an overlay seeded with Erwinia amylovora, the causal agent of fire blight. This zone is caused by two antibiotics, named pantocin A and B. Using a genomic library of Eh318, two cosmids, pCPP702 and pCPP704, were identified that conferred on Escherichia coli the ability to inhibit growth of E. amylovora. The two cosmids conferred different antibiotic activities on E. coli DH5α and had distinct restriction enzyme profiles. A smaller, antibiotic-conferring DNA segment from each cosmid was cloned. Each subclone was characterized and mutagenized with transposons to generate clones that were deficient in conferring pantocin A and B production, respectively. Mutated subclones were introduced into Eh318 to create three antibiotic-defective marker exchange mutants: strain Eh421 (pantocin A deficient); strain Eh439 (pantocin B deficient), and Eh440 (deficient in both pantocins). Cross-hybridization results, restriction maps, and spectrum-of-activity data using the subclones and marker exchange mutants, supported the presence of two distinct antibiotics, pantocin A and pantocin B, whose biosynthetic genes were present in pCPP702 and pCPP704, respectively. The structure of pantocin A is unknown, whereas that of pantocin B has been determined as (R)-N-[((S)-2-amino-propanoylamino)-methyl]-2-methanesulfonyl-succinamic acid. The two pantocins mainly affect other enteric bacteria, based on limited testing. PMID:11133457

  13. Antibiosis activity of Pantoea agglomerans biocontrol strain E325 against Erwinia amylovora on apple flower stigmas.

    Science.gov (United States)

    Pusey, P L; Stockwell, V O; Reardon, C L; Smits, T H M; Duffy, B

    2011-10-01

    Pantoea agglomerans E325, the active ingredient in a commercial product for fire blight control, was previously shown in vitro to produce a unique alkaline- and phosphate-sensitive antibiotic specific to Erwinia amylovora. Antibiosis was evaluated as a mode of antagonism on flower stigmas using two antibiosis-deficient mutants. On King's medium B, mutants E325ad1 and E325ad2 have stable smooth-butyrous or hypermucoid colony morphologies, respectively, and the parental strain E325 exhibits phenotypic plasticity with predominantly hypermucoid colonies accompanied by slower-growing, smooth-butyrous colonies. Mutants were tested against E. amylovora on stigmas of detached flowers of crab apple (Malus mandshurica) in growth chambers and apple (Malus domestica) in the orchard. Epiphytic fitness of the antibiosis-negative mutants was similar or greater than the parental strain as determined by relative area under the population curve (RAUPC). In laboratory and orchard trials, both mutants had significantly lower inhibitory activity against the pathogen (i.e., less reduction of E. amylovora RAUPC) compared with the parental strain. E325 and the mutants caused similar decreases in pH in a broth medium, indicating that acidification, which was previously reported as a possible mechanism of pathogen inhibition on stigmas, is not directly related to antibiosis. In this study we provide the first evidence for E325 antibiosis involved in E. amylovora growth suppression on apple flower stigmas.

  14. Indole affects the formation of multicellular aggregate structures in Pantoea agglomerans YS19.

    Science.gov (United States)

    Yu, Xuemei; Jiang, Jing; Liang, Chen; Zhang, Xiao; Wang, Jieru; Shen, Delong; Feng, Yongjun

    2016-01-01

    Pantoea agglomerans YS19 is an endophytic diazotrophic bacterium isolated from rice. As well as having the ability to form a biofilm, as do most bacteria, it is characterized by the formation of a unique multicellular aggregate structure called symplasmata. Indole is traditionally known as a metabolite of the amino acid tryptophan, which, however, has recently been shown to participate in various regulations of bacterial physiological processes, including stress resistance, quorum sensing and biofilm formation. Here, an indole signal was found to promote symplasmata formation, yet inhibit biofilm formation, indicating different regulatory pathways of indole in the construction of the two structures. However, symplasmata showed almost an equivalent stress-resistant capability, as compared with biofilms, for YS19 to confront acids, heavy metals (Cu(2+)), and UV treatments. Moreover, indole was tested to show a promoting effect on exopolysaccharides (EPS) production and an inhibition effect on the expression of an outer membrane protein OmpW. These results provide evidence for understanding the regulatory mechanisms of indole on such multicellular aggregates.

  15. Phenotypic comparison of clinical and plant-beneficial strains of Pantoea agglomerans.

    Science.gov (United States)

    Bonaterra, Anna; Badosa, Esther; Rezzonico, Fabio; Duffy, Brion; Montesinos, Emilio

    2014-06-01

    Certain strains of Pantoea are used as biocontrol agents for the suppression of plant diseases. However, their commercial registration is hampered in some countries because of biosafety concerns. This study compares clinical and plant-beneficial strains of P. agglomerans and related species using a phenotypic analysis approach in which plant-beneficial effects, adverse effects in nematode models, and toxicity were evaluated. Plant-beneficial effects were determined as the inhibition of apple fruit infection by Penicillium expansum and apple flower infection by Erwinia amylovora. Clinical strains had no general inhibitory activity against infection by the fungal or bacterial plant pathogens, as only one clinical strain inhibited P. expansum and three inhibited E. amylovora. By contrast, all biocontrol strains showed activity against at least one of the phytopathogens, and three strains were active against both. The adverse effects in animals were evaluated in the plant-parasitic nematode Meloidogyne javanica and the bacterial-feeding nematode Caenorhabditis elegans. Both models indicated adverse effects of the two clinical strains but not of any of the plant-beneficial strains. Toxicity was evaluated by means of hemolytic activity in blood, and genotoxicity with the Ames test. None of the strains, whether clinical or plant-beneficial, showed any evidence of toxicity. Copyright© by the Spanish Society for Microbiology and Institute for Catalan Studies.

  16. Separation of Escherichia adecarboxylata from the "Erwinia herbicola-Enterobacter agglomerans" complex and from the other Enterobacteriaceae by nucleic acid and protein electrophoretic techniques.

    Science.gov (United States)

    Izard, D; Mergaert, J; Gavini, F; Beji, A; Kersters, K; De Ley, J; Leclerc, H

    1985-01-01

    The species Escherichia adecarboxylata was examined for DNA relatedness to the "Erwinia herbicola-Enterobacter agglomerans" complex and to other members of the family Enterobacteriaceae. DNA-DNA hybridizations (nitrocellulose filter method) showed that strains received as E. adecarboxylata were highly related to each other (73-100% homology). Three strains of E. agglomerans and one strain of E. herbicola showed, respectively, 77, 96, 97 and 92% relatedness with the labelled DNA of E. adecarboxylata. Two groups (E2 and E3) of "atypical coliforms" previously described by Gavini et al. (1983) showed high reassociation values (76-79% and 80-89%, respectively) with E. adecarboxylata. Most of these strains produced similar or nearly identical protein electrophoregrams. All these strains were therefore classified in E. adecarboxylata. This taxon yielded hybridization values lower than 53% with the previously described phenetic or genetic groups belonging to or related to the "herbicola-agglomerans" complex and values lower than 64% with 56 other species of the Enterobacteriaceae. It was concluded that E. adecarboxylata is a species different from E. agglomerans and the other species of the family Enterobacteriaceae. A new definition of the species E. adecarboxylata is presented.

  17. [Seven cases of port-a-cath contamination caused by Pantoea agglomerans in the Oncological Service of Iseo Hospital, Brescia (Italy)].

    Science.gov (United States)

    Izzo, Ilaria; Lania, Donatella; Castro, Antonino; Lanzini, Fernanda; Bella, Daniele; Pagani, Adriano; Colombini, Paolo

    2014-06-01

    Pantoea agglomerans, a gram negative bacillus in the Enterobacteriaceae family, has been isolated from feculent material, plants and soil. Soft tissue and bone-joint infections due to P. agglomerans following penetrating trauma by vegetation and bacteraemia in association with intravenous fluid, total parenteral nutrition, blood products and anaesthetic agent contamination have been reported. Between October 2009 and January 2010 seven cases of port a cath contamination caused by P. agglomerans were observed in the Oncological Service of our hospital. All patients presented with septic fever after heparinization of the central venous catheter. 5/7 patients were female; mean age was 67 years (range 58-75). 6/7 patients were affected by colorectal adenocarcinoma, 1/7 by mammarian cancer. Mean time from CVC insertion was 23.8 months (range 13-42) at the time of fever. In three cases, port a cath was removed following the oncologist prescription. P. agglomerans was isolated from the catheter tip in one case and from CVC blood culture in 6-7 cases. In all cases peripheral blood cultures were negative. Patients were treated with ciprofloxacin lock therapy and systemic therapy (per os), obtaining negative cultures from port a cath. Notwithstanding the absence of isolation of Pantoea strains from environmental cultures, after educational intervention, which underlined some faulty procedures in CVC management, no further cases were observed.

  18. Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity

    Energy Technology Data Exchange (ETDEWEB)

    Torres, S. K.; Campos, V. L., E-mail: vcampos@udec.cl; Leon, C. G. [Universidad de Concepcion, Laboratorio de Microbiologia Ambiental, Departamento de Microbiologia (Chile); Rodriguez-Llamazares, S. M. [Centro de Investigacion de Polimeros Avanzados (CIPA) (Chile); Rojas, S. M.; Gonzalez, M. [Universidad de Concepcion, Laboratorio de Fisiologia Vascular, Departamento de Fisiologia (Chile); Smith, C. [Universidad de Concepcion, Departamento de Microbiologia (Chile); Mondaca, M. A. [Universidad de Concepcion, Laboratorio de Microbiologia Ambiental, Departamento de Microbiologia (Chile)

    2012-11-15

    The bio-reduction of selenite (Se (IV)) generates nanoparticles with sizes ranging between 30 and 300 nm. Biologic properties of Se nanoparticles, e.g., antioxidant activity, are dependent on the nanoparticle size; smaller particles have greater activity. In this study, the bio-reduction of selenite by Pantoea agglomerans strain UC-32 under aerobic conditions and room temperature to produce bioactive Se nanoparticles smaller than 100 nm was demonstrated. Isolation and purification of the nanoparticles was performed by alkaline lysis. These purified nanoparticles were stabilized with l-cysteine (4 mM). The visualization and characterization of nanoparticles were performed by transmission electron microscopy, energy dispersive X-ray spectroscopy, and scanning electron microscopy. The antioxidant activity of nanoparticles was determined by production of reactive oxygen species using human umbilical vein endothelial cells. Transmission electron microscopy images showed the accumulation of spherical selenium nanoparticles as intracellular and extracellular deposits. The size of Se nanoparticles varied with incubation time. Amorphous Se nanoparticles with size in the order of 100 nm were obtained before 24 h of incubation; but, at 24 h of incubation, the size of the majority of the nanoparticles was in the desirable order of 100 nm and they were not aggregated. Energy dispersive spectroscopy spectra indicated that nanoparticles were composed entirely of selenium. Antioxidant activity of stabilized selenium nanoparticles demonstrated high antioxidant activity when compared to selenite and selenium nanoparticles without stabilization. Stabilized biologically synthetized selenium (0) nanoparticles with size less than 100 nm have a potential application as a food additive with antioxidant properties relevant to human health.

  19. Polar auxin transport is essential for gall formation by Pantoea agglomerans on Gypsophila.

    Science.gov (United States)

    Chalupowicz, Laura; Weinthal, Dan; Gaba, Victor; Sessa, Guido; Barash, Isaac; Manulis-Sasson, Shulamit

    2013-02-01

    The virulence of the bacterium Pantoea agglomerans pv. gypsophilae (Pag) on Gypsophila paniculata depends on a type III secretion system (T3SS) and its effectors. The hypothesis that plant-derived indole-3-acetic acid (IAA) plays a major role in gall formation was examined by disrupting basipetal polar auxin transport with the specific inhibitors 2,3,5-triiodobenzoic acid (TIBA) and N-1-naphthylphthalamic acid (NPA). On inoculation with Pag, galls developed in gypsophila stems above but not below lanolin rings containing TIBA or NPA, whereas, in controls, galls developed above and below the rings. In contrast, TIBA and NPA could not inhibit tumour formation in tomato caused by Agrobacterium tumefaciens. The colonization of gypsophila stems by Pag was reduced below, but not above, the lanolin-TIBA ring. Following Pag inoculation and TIBA treatment, the expression of hrpL (a T3SS regulator) and pagR (a quorum-sensing transcriptional regulator) decreased four-fold and that of pthG (a T3SS effector) two-fold after 24 h. Expression of PIN2 (a putative auxin efflux carrier) increased 35-fold, 24 h after Pag inoculation. However, inoculation with a mutant in the T3SS effector pthG reduced the expression of PIN2 by two-fold compared with wild-type infection. The results suggest that pthG might govern the elevation of PIN2 expression during infection, and that polar auxin transport-derived IAA is essential for gall initiation. © 2012 THE AUTHORS. MOLECULAR PLANT PATHOLOGY © 2012 BSPP AND BLACKWELL PUBLISHING LTD.

  20. Osteomyelitis caused by Pantoea agglomerans in a closed fracture in a child.

    Science.gov (United States)

    Labianca, Luca; Montanaro, Antonello; Turturro, Francesco; Calderaro, Cosma; Ferretti, Andrea

    2013-02-01

    Development of osteomyelitis in a closed fracture is rare. Although it has been reported that trivial trauma may be associated with the subsequent development of acute osteomyelitis, no evidence has been found that fractures are involved in the etiology of osteomyelitis. Only 25 cases (11 in adolescents) of osteomyelitis after closed fracture have been reported in literature. The authors report a case of osteomyelitis in an 8-year-old girl after a forearm fracture involving the diaphysis of the radius and ulna. Radiographic examinations were performed 5, 14, and 30 days after trauma. At last examination, the forearm appeared swollen and red. Blood culture and radiographs confirmed the clinical suspicion of osteomyelitis. The cultures grew Pantoea agglomerans, a saprophytic gram-negative bacterium commonly isolated from plants, flowers, seeds, water, and soil and sensitive to all of the most common antibiotics. After the arm was immobilized, high doses of intravenous antibiotics were administered for 4 weeks, and oral antibiotics were prescribed for an additional 4 weeks. The patient responded well to treatment. Twelve months after the initial injury, the patient regained full elbow and wrist flexion-extension range of motion. Based on their experience and a review of the literature, the authors suggest that bone infection be considered for patients with closed fractures complicated by excessive or prolonged pain and swelling with evident signs of inflammation where no other obvious infection is apparent. Moreover, in children, bacterial infection may cause osteomyelitis more frequently than in adults, but no evidence exists that a closed fracture increases the incidence of osteomyelitis in children with a systemic infection. Copyright 2013, SLACK Incorporated.

  1. Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans.

    Science.gov (United States)

    Lemieszek, Marta Kinga; Chilosi, Marco; Golec, Marcin; Skórska, Czesława; Dinnyes, Andras; Mashayekhi, Kaveh; Vierlinger, Klemens; Huaux, Francois; Wielscher, Matthias; Hofner, Manuela; Yakoub, Yousof; Pastena, Chiara; Daniele, Isabella; Cholewa, Grażyna; Sitkowska, Jolanta; Lisowska, Wiesława; Zwoliński, Jacek; Milanowski, Janusz; Mackiewicz, Barbara; Góra-Florek, Anna; Ziesche, Rolf; Dutkiewicz, Jacek

    2013-09-01

    Hypersensitivity pneumonitis (HP) represents the immunologically mediated lung disease induced by repeated inhalations of a wide variety of certain finely dispersed organic antigens. In susceptible subjects, these inhalations provoke a hypersensitivity reaction characterized by intense inflammation of the terminal bronchioles, the interstitium and the alveolar tree. The inflammation often organizes into granulomas and may progress to pulmonary fibrosis. Our previous work indicated that cell extract of gram-negative bacteria Pantoea agglomerans (SE-PA) causes, in young C57BL/6J mice, pulmonary changes that are very similar to the clinical manifestations of HP in men. The purpose of presented studies was to describe the response of mice immune system while exposed to SE-PA. Particular attention was paid to examine the age influence on SE-PA induced inflammation and fibrosis in lung tissue. We used 3- and 18-month-old C57BL/6J mice. Lung samples were collected from untreated mice and animals exposed to harmful agent for 7 and 28 days. HP development was monitored by histological and biochemical evaluation. Using ELISA tests, we examined concentration of pro- and anti-inflammatory cytokines in lung homogenates. Our study demonstrated again that SE-PA provokes in mice changes typical for the clinical picture of HP, and that successive stages of disease (acute, subacute and chronic) might be obtained by modulation of time exposure. Furthermore, we found that animals' age at the time of sensitization influences the nature of observed changes (cytokine expression pattern) and the final outcome (reaction intensity and scale of fibrosis).

  2. Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity

    Science.gov (United States)

    Torres, S. K.; Campos, V. L.; León, C. G.; Rodríguez-Llamazares, S. M.; Rojas, S. M.; González, M.; Smith, C.; Mondaca, M. A.

    2012-11-01

    The bio-reduction of selenite (Se (IV)) generates nanoparticles with sizes ranging between 30 and 300 nm. Biologic properties of Se nanoparticles, e.g., antioxidant activity, are dependent on the nanoparticle size; smaller particles have greater activity. In this study, the bio-reduction of selenite by Pantoea agglomerans strain UC-32 under aerobic conditions and room temperature to produce bioactive Se nanoparticles smaller than 100 nm was demonstrated. Isolation and purification of the nanoparticles was performed by alkaline lysis. These purified nanoparticles were stabilized with l-cysteine (4 mM). The visualization and characterization of nanoparticles were performed by transmission electron microscopy, energy dispersive X-ray spectroscopy, and scanning electron microscopy. The antioxidant activity of nanoparticles was determined by production of reactive oxygen species using human umbilical vein endothelial cells. Transmission electron microscopy images showed the accumulation of spherical selenium nanoparticles as intracellular and extracellular deposits. The size of Se nanoparticles varied with incubation time. Amorphous Se nanoparticles with size in the order of 100 nm were obtained before 24 h of incubation; but, at 24 h of incubation, the size of the majority of the nanoparticles was in the desirable order of 100 nm and they were not aggregated. Energy dispersive spectroscopy spectra indicated that nanoparticles were composed entirely of selenium. Antioxidant activity of stabilized selenium nanoparticles demonstrated high antioxidant activity when compared to selenite and selenium nanoparticles without stabilization. Stabilized biologically synthetized selenium (0) nanoparticles with size less than 100 nm have a potential application as a food additive with antioxidant properties relevant to human health.

  3. Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity

    International Nuclear Information System (INIS)

    Torres, S. K.; Campos, V. L.; León, C. G.; Rodríguez-Llamazares, S. M.; Rojas, S. M.; González, M.; Smith, C.; Mondaca, M. A.

    2012-01-01

    The bio-reduction of selenite (Se (IV)) generates nanoparticles with sizes ranging between 30 and 300 nm. Biologic properties of Se nanoparticles, e.g., antioxidant activity, are dependent on the nanoparticle size; smaller particles have greater activity. In this study, the bio-reduction of selenite by Pantoea agglomerans strain UC-32 under aerobic conditions and room temperature to produce bioactive Se nanoparticles smaller than 100 nm was demonstrated. Isolation and purification of the nanoparticles was performed by alkaline lysis. These purified nanoparticles were stabilized with l-cysteine (4 mM). The visualization and characterization of nanoparticles were performed by transmission electron microscopy, energy dispersive X-ray spectroscopy, and scanning electron microscopy. The antioxidant activity of nanoparticles was determined by production of reactive oxygen species using human umbilical vein endothelial cells. Transmission electron microscopy images showed the accumulation of spherical selenium nanoparticles as intracellular and extracellular deposits. The size of Se nanoparticles varied with incubation time. Amorphous Se nanoparticles with size in the order of 100 nm were obtained before 24 h of incubation; but, at 24 h of incubation, the size of the majority of the nanoparticles was in the desirable order of 100 nm and they were not aggregated. Energy dispersive spectroscopy spectra indicated that nanoparticles were composed entirely of selenium. Antioxidant activity of stabilized selenium nanoparticles demonstrated high antioxidant activity when compared to selenite and selenium nanoparticles without stabilization. Stabilized biologically synthetized selenium (0) nanoparticles with size less than 100 nm have a potential application as a food additive with antioxidant properties relevant to human health.

  4. Molecular epidemiology of a nosocomial outbreak due to Enterobacter cloacae and Enterobacter agglomerans in Campinas, São Paulo, Brazil.

    Science.gov (United States)

    Gonçalves, C R; Vaz, T M; Leite, D; Pisani, B; Simoes, M; Prandi, M A; Rocha, M M; Cesar, P C; Trabasso, P; von Nowakonski, A; Irino, K

    2000-01-01

    A total of 73 isolates (57 Enterobacter cloacae and 16 Enterobacter agglomerans), recovered during an outbreak of bacteremia in the Campinas area, São Paulo, Brazil, were studied. Of these isolates, 61 were from parenteral nutrition solutions, 9 from blood cultures, 2 from a sealed bottle of parenteral nutrition solution, and one was of unknown origin. Of the 57 E. cloacae isolates, 54 were biotype 26, two were biotype 66 and one was non-typable. Of 39 E. cloacae isolates submitted to ribotyping, 87.2% showed the same banding pattern after cleavage with EcoRI and BamHI. No important differences were observed in the antimicrobial susceptibility patterns among E. cloacae isolates exhibiting the same biotype, serotype and ribotype. All E. agglomerans isolates, irrespective of their origin, showed same patterns when cleaved with EcoRI and BamHI. The results of this investigation suggest an intrinsic contamination of parenteral nutrition solutions and incriminate these products as a vehicle of infection in this outbreak.

  5. Effect of gamma-irradiation on membrane fatty acids and peptidoglycan's muropeptides of Pantoea agglomerans, a plant pathogen.

    Science.gov (United States)

    Dussault, D; Caillet, S; Le Tien, C; Lacroix, M

    2009-03-01

    The aim of this study was to evaluate the effect of gamma-irradiation on the fatty acids (FA) and muropeptides content of two strains of an Enterobacteriacea: Pantoea agglomerans. Pantoea agglomerans strains ATCC 49174 and RL1 isolated from irradiated carrots were used for this study. Radiation treatments (1 and 3.5 kGy) were performed to study the radiotolerance. Total lipids were obtained by multiple extractions using methanol/chloroform (2 : 1) and were quantified by GC. Muropeptides were purified by successive enzymatic digestions and analysed using a reverse phase C(18) column in high performance liquid chromatography. A significant (P composition and the muropeptides. Effects of irradiation on the bacterial membrane are noticeable and could play an important role on the cellular response and ability to survive this harsh environment. To our knowledge, it is the first study to demonstrate the effects of ionizing irradiation on the modification of the FA and one of the few to confirm its effects on the muropeptides of the peptidoglycan.

  6. Rhizosphere competent Pantoea agglomerans enhances maize (Zea mays) and chickpea (Cicer arietinum L.) growth, without altering the rhizosphere functional diversity.

    Science.gov (United States)

    Mishra, Aradhana; Chauhan, Puneet Singh; Chaudhry, Vasvi; Tripathi, Manisha; Nautiyal, Chandra Shekhar

    2011-10-01

    Plant growth promoting Pantoea agglomerans NBRISRM (NBRISRM) was able to produce 60.4 μg/ml indole acetic acid and solubilize 77.5 μg/ml tri-calcium phosphate under in vitro conditions. Addition of 2% NaCl (w/v) in the media induced the IAA production and phosphate solubilization by 11% and 7%, respectively. For evaluating the plant growth promotory effect of NBRISRM inoculation a micro plot trial was conducted using maize and chickpea as host plants. The results revealed significant increase in all growth parameters tested in NBRISRM inoculated maize and chickpea plants, which were further confirmed by higher macronutrients (N, P and K) accumulation as compared to un-inoculated controls. Throughout the growing season of maize and chickpea, rhizosphere population of NBRISRM were in the range 10(7)-10(8) CFU/g soil and competing with 10(7)-10(9) CFU/g soil with heterogeneous bacterial population. Functional richness, diversity, and evenness were found significantly higher in maize rhizosphere as compared to chickpea, whereas NBRISRM inoculation were not able to change it, in both crops as compared to their un-inoculated control. To the best of our knowledge this is first report where we demonstrated the effect of P. agglomerans strain for improving maize and chickpea growth without altering the functional diversity.

  7. Bacteriophages LIMElight and LIMEzero of Pantoea agglomerans, Belonging to the “phiKMV-Like Viruses” ▿ †

    Science.gov (United States)

    Adriaenssens, Evelien M.; Ceyssens, Pieter-Jan; Dunon, Vincent; Ackermann, Hans-Wolfgang; Van Vaerenbergh, Johan; Maes, Martine; De Proft, Maurice; Lavigne, Rob

    2011-01-01

    Pantoea agglomerans is a common soil bacterium used in the biocontrol of fungi and bacteria but is also an opportunistic human pathogen. It has been described extensively in this context, but knowledge of bacteriophages infecting this species is limited. Bacteriophages LIMEzero and LIMElight of P. agglomerans are lytic phages, isolated from soil samples, belonging to the Podoviridae and are the first Pantoea phages of this family to be described. The double-stranded DNA (dsDNA) genomes (43,032 bp and 44,546 bp, respectively) encode 57 and 55 open reading frames (ORFs). Based on the presence of an RNA polymerase in their genomes and their overall genome architecture, these phages should be classified in the subfamily of the Autographivirinae, within the genus of the “phiKMV-like viruses.” Phylogenetic analysis of all the sequenced members of the Autographivirinae supports the classification of phages LIMElight and LIMEzero as members of the “phiKMV-like viruses” and corroborates the subdivision into the different genera. These data expand the knowledge of Pantoea phages and illustrate the wide host diversity of phages within the “phiKMV-like viruses.” PMID:21421778

  8. Efficient synthesis of hydroxystyrenes via biocatalytic decarboxylation/deacetylation of substituted cinnamic acids by newly isolated Pantoea agglomerans strains.

    Science.gov (United States)

    Sharma, Upendra K; Sharma, Nandini; Salwan, Richa; Kumar, Rakesh; Kasana, Ramesh C; Sinha, Arun K

    2012-02-01

    Decarboxylation of substituted cinnamic acids is a predominantly followed pathway for obtaining hydroxystyrenes-one of the most extensively explored bioactive compounds in the food and flavor industry (e.g. FEMA GRAS approved 4-vinylguaiacol). For this, mild and green strategies providing good yields with high product selectivity are needed. Two newly isolated bacterial strains, i.e. Pantoea agglomerans KJLPB4 and P. agglomerans KJPB2, are reported for mild and effective decarboxylation of substituted cinnamic acids into corresponding hydroxystyrenes. Key operational parameters for the process, such as incubation temperature, incubation time, substrate concentration and effect of co-solvent, were optimized using ferulic acid as a model substrate. With strain KJLPB4, 1.51 g L⁻¹ 4-vinyl guaiacol (98% yield) was selectively obtained from 2 g L⁻¹ ferulic acid at 28 °C after 48 h incubation. However, KJPB2 provided vanillic acid in 85% yield after 72 h following the oxidative decarboxylation pathway. In addition, KJLPB4 was effectively exploited for the deacetylation of acetylated α-phenylcinnamic acids, providing corresponding compounds in 65-95% yields. Two newly isolated microbial strains are reported for the mild and selective decarboxylation of substituted cinnamic acids into hydroxystyrenes. Preparative-scale synthesis of vinyl guaiacol and utilization of renewable feedstock (ferulic acid extracted from maize bran) have been demonstrated to enhance the practical utility of the process. Copyright © 2011 Society of Chemical Industry.

  9. Control of Diatraea saccharalis by the endophytic Pantoea agglomerans 33.1 expressing cry1Ac7.

    Science.gov (United States)

    Quecine, M C; Araújo, W L; Tsui, S; Parra, J R P; Azevedo, J L; Pizzirani-Kleiner, A A

    2014-04-01

    Despite the fact that Bacillus thuringiensis (Bt) is found in more than 90 % of the products used against insects, it has some difficulty reaching the internal regions where the larvae feed. To solve this problem, many genetically modified microorganisms that colonize the same pests have been developed. Thus, the endophytic bacterium Pantoea agglomerans (33.1), which has been recently described as a promising sugarcane growth promoter, was genetically modified with the pJTT vector (which carries the gene cry1Ac7) to control the sugarcane borer, Diatraea saccharalis. Firstly, the bioassays for D. saccharalis control by 33.1:pJTT were conducted with an artificial diet. A new in vivo methodology was also developed, which confirmed the partial control of larvae by 33.1:pJTT. The 33.1:pJTT strain was inoculated into sugarcane stalks containing the D. saccharalis larvae. In the sugarcane stalks, 33.1:pJTT was able to increase the mortality of D. saccharalis larvae, impair larval development and decrease larval weight. Sugarcane seedlings were inoculated with 33.1:pJTT, and re-isolation confirmed the capacity of 33.1:pJTT to continuously colonize the sugarcane. These results prove that P. agglomerans (33.1), a sugarcane growth promoter, can be improved by expressing the Cry protein, and the resulting strain is able to control the sugarcane borer.

  10. Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector

    Science.gov (United States)

    de Lima Procópio, Rudi Emerson; Araújo, Welington Luiz; Andreote, Fernando Dini; Azevedo, João Lúcio

    2011-01-01

    A circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP), and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14 days after inoculation, the strain occupied the inner tissue of Eucalyptus grandis roots, preferentially colonizing the xylem vessels of the host plants. PMID:21637551

  11. Thermodynamic and kinetic controls on cotransport of Pantoea agglomerans cells and Zn through clean and iron oxide coated sand columns.

    Science.gov (United States)

    Kapetas, Leon; Ngwenya, Bryne T; Macdonald, Alan M; Elphick, Stephen C

    2012-12-18

    Recent observations that subsurface bacteria quickly adsorb metal contaminants raise concerns that they may enhance metal transport, given the high mobility of bacteria themselves. However, metal adsorption to bacteria is also reversible, suggesting that mobility within porous medium will depend on the interplay between adsorption-desorption kinetics and thermodynamic driving forces for adsorption. Till now there has been no systematic investigation of these important interactions. This study investigates the thermodynamic and kinetic controls of cotransport of Pantoea agglomerans cells and Zn in quartz and iron-oxide coated sand (IOCS) packed columns. Batch kinetic studies show that significant Zn sorption on IOCS takes place within two hours. Adsorption onto P. agglomerans surfaces reaches equilibrium within 30 min. Experiments in flow through quartz sand systems demonstrate that bacteria have negligible effect on zinc mobility, regardless of ionic strength and pH conditions. Zinc transport exhibits significant retardation in IOCS columns at high pH in the absence of cells. Yet, when mobile bacteria (non attached) are passed through simultaneously with zinc, no facilitated transport is observed. Adsorption onto cells becomes significant and plays a role in mobile metal speciation only once the IOCS is saturated with zinc. This suggests that IOCS exhibits stronger affinity for Zn than cell surfaces. However, when bacteria and Zn are preassociated on entering the column, zinc transport is initially facilitated. Subsequently, zinc partly desorbs from the cells and redistributes onto the IOCS as a result of the higher thermodynamic affinity for IOCS.

  12. Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector

    Directory of Open Access Journals (Sweden)

    Procópio Rudi Emerson de Lima

    2011-01-01

    Full Text Available A circular cryptic plasmid named pPAGA (2,734 bp was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus. Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A, 250(B, 131(C, and 129(D amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP, and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14 days after inoculation, the strain occupied the inner tissue of Eucalyptus grandis roots, preferentially colonizing the xylem vessels of the host plants.

  13. Educação Empreendedora nos Ensinos Médio e Fundamental: Diversas Percepções

    Directory of Open Access Journals (Sweden)

    Silvana Neumann Martins

    2016-07-01

    Full Text Available Este estudo objetivou conhecer as percepções e os conceitos dos professores de Ciências Exatas sobre educação empreendedora. A pesquisa constitui-se em um estudo de caso com abordagem qualitativa. As informações foram obtidas por meio de entrevistas semiestruturadas com 6 professores de Ciências Exatas, que atuam em escolas de três municípios do Vale do Taquari/RS/BR. A análise dos enunciados seguiu as orientações metodológicas da análise textual discursiva - ATD. Os resultados que emergiram apontam que 5 professores conhecem os conceitos que norteiam a educação empreendedora e ministram práticas pedagógicas inovadoras em suas aulas, levando seus alunos a aprender a empreender, contribuindo para a melhoria do ensino de Ciências Exatas.

  14. Composição mineral de diversas hortaliças Mineral composition of several vegetable crops

    Directory of Open Access Journals (Sweden)

    A. M. C. Furlani

    1978-01-01

    Full Text Available Objetivou-se conhecer, na época normal de colheita, as quantidades de matéria seca acumuladas, as concentrações dos elementos essenciais às plantas, mais as de cobalto, alumínio e sódio na matéria fresca e seca de 50 cultivares de hortaliças num total de 35 espécies. As amostras, normalmente constituídas de produtos da colheita, foram na sua maioria procedentes da região de Campinas, Estado de São Paulo e separadas em: melancia - em casca + polpa branca, polpa vermelha e semente; melão - em casca + polpa e semente; ervilha - em vagem e grão; berinjela - em fruto e pedúnculo; couve-flor - em folha e inflorescência; beterraba, cenoura, nabo e rabanete - em folha e raiz; alcachofra - em folha + caule e inflorescência. Das demais hortaliças foram utilizados o fruto todo, as folhas ou os bulbos sem separação. Verificou-se que as leguminosas extraíram maiores quantidades de N, P, K, Mg, Cu, Mo, Zn e Co; as tuberosas, de Cl, Fe e Mn; as amarilidáceas, de S, B e Al; as folhosas, de Ca e Na. As cucurbitáceas extraíram menores quantidades da maioria dos nutrientes.This work reports the dry matter accumulation, the nutrient, Co, Al and Na - concentrations, and the removal of nutrients and Co, Al and Na per metric ton of fresh material of 35 species of vegetables by the harvest. The samples mostly came from the region of Campinas, State of São Paulo and consisted of the products removed from the field, fractioned in their several parts: watermelon in epicarp + white pulp, red pulp and seeds; eggplant in fruit and stalk; melon in epicarp + pulp and seeds; pea in shell and seeds; cauliflower in leaf and inflorescence; sugar beet, carrot, turnip and radish in leaf and root; artichoke in leaf + stalk and inflorescence. The whole fruit was utilized in the case of edible fruit bearing vegetables; the leaves in the case of the leafy vegetables; and the bulbous root in the case of the iffy-like vegetables. In general, the leguminous

  15. Denitration of glycerol trinitrate by resting cells and cell extracts of Bacillus thuringiensis/cereus and Enterobacter agglomerans.

    Science.gov (United States)

    Meng, M; Sun, W Q; Geelhaar, L A; Kumar, G; Patel, A R; Payne, G F; Speedie, M K; Stacy, J R

    1995-07-01

    A number of microorganisms were selected from soil and sediment samples which were known to have been previously exposed to nitrate ester contaminants. The two most effective bacteria for transforming glycerol trinitrate (GTN) were identified as Bacillus thuringiensis/cereus and Enterobacter agglomerans. For both isolates, denitration activities were expressed constitutively and GTN was not required for induction. Dialysis of cell extracts from both isolates did not affect denitration, which indicates that dissociable and depletable cofactors are not required for denitration. With thin-layer chromatography and high-performance liquid chromatography, the denitration pathway for both isolates was shown to be a sequential denitration of GTN to glycerol dinitrate isomers, glycerol mononitrate isomers, and ultimately to glycerol. GTN was observed to be completely converted to glycerol during a long-term incubation of cell extracts.

  16. In vitro adsorption revealing an apparent strong interaction between endophyte Pantoea agglomerans YS19 and host rice.

    Science.gov (United States)

    Miao, Yuxuan; Zhou, Jia; Chen, Cuicui; Shen, Delong; Song, Wei; Feng, Yongjun

    2008-12-01

    Pantoea (formerly Enterobacter) agglomerans YS19 is a dominant diazotrophic endophyte isolated from rice (Oryza sativa cv. Yuefu) grown in a temperate-climate region in west Beijing, China. In vitro adsorption and invasion of YS19 on host plant root were studied in this research. Adsorption of YS19 on rice seedling roots closely resembled the Langmuir adsorption and showed a higher adsorption quantity than the control strains Paenibacillus polymyxa WY110 (a rhizospheric bacterium from the same rice cultivar) and Escherichia coli HB101 (a general model bacterium). Adsorption dynamics study revealed high rates and a long duration of the YS19-rice root adsorption process. Adsorption of YS19 was mainly observed on the root hair, though which it enters the plant. This in vitro adsorption study revealed an apparent strong interaction between YS19 and rice at the early endophyte-host recognition stage.

  17. Isolation and Identification of Two l-Azetidine-2-carboxylic Acid-Degrading Soil Microorganisms, Enterobacter agglomerans and Enterobacter amnigenus

    Science.gov (United States)

    Yeung; Lee; Woodard

    1998-02-27

    Soil samples collected at several times during the growing season and at different locations within Convallaria majalis beds in Ann Arbor, MI, were screened for their ability to grow with the cyclic amino acid, l-azetidine-2-carboxylic acid (l-A-2-C), as their sole nitrogen source (i.e., metabolize l-A-2-C). Two different soil microorganisms were isolated, characterized, and identified using fundamental selection methods, the standard battery of biochemical characterization tests, and scanning electron microscopy. The assignment of the identity of these organisms as Enterobacter agglomerans and Enterobacter amnigenus was further verified by comparison with authentic microbial samples obtained from ATCC that were able to utilize l-A-2-C as their sole nitrogen source.

  18. Densidad mineral ósea en una muestra de jóvenes chilenos practicantes de diversas modalidades deportivas

    Directory of Open Access Journals (Sweden)

    Rossana Gómez-Campos

    2017-01-01

    Full Text Available Objetivos: Objetivos: a Comparar la densidad mineral ósea de una muestra de jóvenes chilenos practicantes de diversas modalidades deportivas y b Analizar la densidad mineral ósea en función de la maduración biológica. Métodos: Se estudiaron 146 adolescentes de sexo masculino, con un rango de edad entre 10 a 18 años. Se organizaron cinco grupos de trabajo: Grupo control (escolares n= 40, Canotaje (n= 30, Ciclismo (n=14, Fútbol (n=28 y Natación (n=34. Se evaluó el peso, estatura, altura tronco-cefálica. Se calculó el índice de Masa Corporal y la maduración biológica por medio de años de pico de velocidad de crecimiento. La densidad mineral ósea de cuerpo total y el porcentaje de grasa corporal se determinó por medio de la absorciometría de rayos X de doble energía. Resultados: Los adolescentes que practicaban fútbol evidenciaron mayor densidad mineral ósea (1,23±0,12g/cm 2 en relación a los jóvenes del ciclismo (0,99±0,11g/cm 2 , canotaje (1,09±0,17g/cm 2 , natación (1,10±0,11g/cm 2 y al grupo control de escolares (1,04±0,14g/ cm 2 (p<0.001. Hubo diferencias entre los tres niveles de maduración biológica en las cuatro modalidades deportivas y en el grupo control (p<0.001. La mayor densidad mineral ósea en función de la maduración somática se observó en los futbolistas. Conclusión: Los adolescentes que practican fútbol evidenciaron mayor densidad mineral ósea con relación a las demás modalidades deportivas y con relación al grupo control, además la maduración somática juega un papel relevante en el incremento de densidad mineral ósea, en especial en los futbolistas. Los resultados sugieren desarrollar actividades físico- deportivas de alto impacto antes, durante y después de producirse la maduración biológica en adolescentes de edad escolar.

  19. Trayectorias del Sur: Desplazamientos y conformaciones de las naciones diversas de Ecuador y Etiopía

    Directory of Open Access Journals (Sweden)

    Cristina Cielo

    2015-12-01

    Full Text Available Este artículo se centra en un análisis comparativo de las distintas políticas de gestión de la diversidad nacional de los Estados de Ecuador y Etiopía. Se argumenta que dichas políticas han sido configuradas en gran medida por la influencia de diálogos internacionales en torno a las diversas desigualdades, la conceptualización de lo indígena y lo étnico, y acerca de la coexistencia nacional entre grupos históricamente jerarquizados en estructuras políticas y sociales de poder. Puesto que las discusiones y debates sobre la institucionalización de la nación diversaestán insertas en problemáticas políticas y disputas ideológicas, es necesario conocer los regímenes de saber en los que se produce esta institucionalización. En el caso de Ecuador, el proyecto político de laplurinacionalidad se gestó a partir de debates intelectuales en el seno del marxismo, especialmente en torno a la relación entre raza y clase. A esta aportación inicial vendrían a sumarse las generadas en encuentros regionales en los que se definieron reivindicaciones relativas a los derechos de los pueblos y naciones indígenas. En el caso de Etiopía, sería la incorporación de teorías y estrategias nacidas de la lucha anti-colonial en África y Asia las que marcarían el discurrir del tipo específico de gestión de la diversidad nacional, encaminándose finalmente hacia la constitución de unafederación étnica. De una primigenia unión interétnica anti-imperial, en el contexto del régimen militar-autoritario delDerg y ante la creciente importancia de la migración en la política etíope, mutaría hacia el polo opuesto, favoreciendo y reforzando la división étnica.

  20. Microbial Fe (III) reduction and hydrogen production by a transposon-mutagenized strain of Pantoea agglomerans BH18

    International Nuclear Information System (INIS)

    Liu, Hongyan; Wang, Guangce

    2015-01-01

    Based on the transposon-mutagenized library of Pantoea agglomerans BH18, mutant screens were conducted to obtain the strain with the highest Fe (III) reduction and hydrogen production. Of these transposon-mutagenized mutants, the mutant strain TB230 was screened for high Fe (III)-reducing efficiency and hydrogen production. The PCR amplification and kanamycin resistance selection results indicated that the transposon insertion of the mutant strain TB230 was stable. Hydrogen production of the mutant strain TB230 was (2.21 ± 0.34) mol H 2 /mol glucose, which increased hydrogen production by over 40% compared with that of the wild type strain. The accumulation concentration of Fe (II) in the medium of the mutant strain TB230 with Fe (OH) 3 as the sole electron acceptor was (7.39 ± 0.49) mmol/l, which was approximately 3-fold greater than that of the wild type strain. The mutant strain TB230 showed high Fe (III)-reducing activity and hydrogen production by adopting glucose and pyruvate as the carbon source. In addition, the mutant strain TB230 was capable of Fe (III) reduction and hydrogen production under fresh or marine conditions. This result indicates that the mutant strain with high microbial Fe (III) reduction and hydrogen production is beneficial for the improvement of anaerobic performance. - Highlights: • The mutant strain TB230 was a transposon-mutagenized strain of Pantoea agglomerans BH18. • Strain TB230 was screened for high Fe (III)-reducing efficiency and hydrogen production. • H 2 yield and Fe (III)-reducing activity were 2.21 ± 0.34 and 7.39 ± 0.49 in marine condition. • Strain TB230 was capable of Fe (III) reduction and hydrogen production in fresh or marine condition

  1. Immunopotentiator from Pantoea agglomerans 1 (IP-PA1) Promotes Murine Hair Growth and Human Dermal Papilla Cell Gene Expression.

    Science.gov (United States)

    Wakame, Koji; Okawa, Hiroshi; Komatsu, Ken-Ich; Nakata, Akifumi; Sato, Keisuke; Ingawa, Hiroyuki; Kohchi, Chie; Nishizawa, Takashi; Soma, Gen-Ichiro

    2016-07-01

    The lipopolysaccharide (LPS)-like compound derived from Pantoea agglomerans (immunopotentiator from Pantoea agglomerans 1 (IP-PA1)) has been used not only as dietary supplement or cosmetic for humans, but also by Japanese veterinarians as an anti-tumor, anti-allergy, "keep a fine coat of fur" and hair growth-promoting functional food for dogs and cats. In the present study, we focused on the hair growth-promoting effects of IP-PA1 on a hair-shaved animal model and its mechanism of action. We also investigated its potential on gene expression after stimulating human dermal papilla cells with IP-PA1. The hair on the back of a C3H/HeN mouse was shaved and IP-PA1 was orally administered or applied to the skin. The status of hair growth was observed and recorded for 14 days. Skin was collected and histological tissue examination was performed with respect to hair growth status using hematoxylin and eosin staining. After IP-PA1 administration (2 and 10 μg/ml) to human dermal papilla cell culture system for 24 h, fibroblast growth factor-7 (FGF-7) and vascular endothelial growth factor (VEGF) mRNA expression were measured using real-time polymerase chain reaction (PCR) analysis. IP-PA1, when given orally, showed a tendency to promote hair growth in mice. In addition, skin application also significantly promoted hair growth, while histopathological examinations further demonstrated hair elongation from dermal papilla cells. In the human dermal papilla cell culture system, significant FGF-7 and VEGF mRNA expressions were observed (p<0.05). An underlying mechanism of gene expression by which IP-PA1 promotes hair growth was suggested to be different from that of medicine and traditional hair tonics, such as minoxidil and adenosine. Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  2. Global regulatory networks control the hrp regulon of the gall-forming bacterium Pantoea agglomerans pv. gypsophilae.

    Science.gov (United States)

    Panijel, Mary; Chalupowicz, Laura; Sessa, Guido; Manulis-Sasson, Shulamit; Barash, Isaac

    2013-09-01

    Gall formation by Pantoea agglomerans pv. gypsophilae is dependent on the hypersensitive response and pathogenicity (hrp) system. Previous studies demonstrated that PagR and PagI, regulators of the quorum-sensing system, induce expression of the hrp regulatory cascade (i.e., hrpXY, hrpS, and hrpL) that activates the HrpL regulon. Here, we isolated the genes of the Gac/Rsm global regulatory pathway (i.e., gacS, gacA, rsmB, and csrD) and of the post-transcriptional regulator rsmA. Our results demonstrate that PagR and PagI also upregulate expression of the Gac/Rsm pathway. PagR acts as a transcriptional activator of each of the hrp regulatory genes and gacA in a N-butanoyl-L-homoserine lactone-dependent manner as shown by gel shift experiments. Mutants of the Gac/Rsm genes or overexpression of rsmA significantly reduced Pantoea agglomerans virulence and colonization of gypsophila. Overexpression of rsmB sRNA abolished gall formation, colonization, and hypersensitive reaction on nonhost plants and prevented transcription of the hrp regulatory cascade, indicating a lack of functional type III secretion system. Expression of rsmB sRNA in the background of the csrD null mutant suggests that CsrD may act as a safeguard for preventing excessive production of rsmB sRNA. Results presented indicate that the hrp regulatory cascade is controlled directly by PagR and indirectly by RsmA, whereas deficiency in RsmA activity is epistatic to PagR induction.

  3. Optimization of packaging and storage conditions of a freeze-dried Pantoea agglomerans formulation for controlling postharvest diseases in fruit.

    Science.gov (United States)

    Torres, R; Solsona, C; Viñas, I; Usall, J; Plaza, P; Teixidó, N

    2014-07-01

    This study aimed to evaluate different packaging strategies to extend the shelf life of a freeze-dried formulation of the biocontrol agent Pantoea agglomerans strain CPA-2. Different materials and atmosphere packaging conditions (vacuum and air) were analysed on formulated P. agglomerans cells stored at 25, 5 and -20°C. Results showed the viability of CPA-2 cells stored at 5 or -20°C was significantly higher than when stored at 25°C. The highest viabilities were observed with the plastic material designated as Bottle 1, in nonvacuum packaging in all storage temperatures: 50% after 3 months at 25°C, 100% after 8 months at 5°C and 100 and 74% after 12 and 18 months, respectively, at -20°C; the final concentration was 10(12) CFU g(-1), a good concentration for a commercial product. The efficacy to control blue and green mould on apples and oranges, respectively, of these packed and stored cells was similar to fresh CPA-2 cells. This work showed a suitable packaging strategy for a freeze-dried formulation of the CPA-2, providing a good shelf life and efficacy against the major postharvest diseases of apples and citrus based on a plastic bottle stored at cold or frozen storage conditions. The last phase of the commercial development process for biocontrol agents is presented in this work. A bacterium-based product that ensures the efficacy, stability and easy application of the antagonist to control postharvest fungal diseases on fruit was successfully obtained. © 2014 The Society for Applied Microbiology.

  4. Immunopotentiator from Pantoea agglomerans Prevents Atopic Dermatitis Induced by Dermatophagoides farinae Extract in NC/Nga Mouse.

    Science.gov (United States)

    Wakame, Koji; Komatsu, Ken-Ichi; Inagawa, Hiroyuki; Nishizawa, Takashi

    2015-08-01

    Pantoea agglomerans LPS (immunopotentiator from Pantoea agglomerans 1: IP-PA1) has been reported to have anti-inflammatory effects in in vitro and in vivo models. The aim of the present study was to investigate the effects of orally-administered IP-PA1 on atopic dermatitis (AD) symptoms induced by Dermatophagoides farinae body extract (DFE) in NC/Nga mice. Using the NC/Nga AD murine model, mice were orally administered 0.1% (High) or 0.01% (Low) water-containing IP-PA1. Skin lesion assessment and blood collection from the caudal vein was performed on days 0, 7, 21 and 31. On day 31, all mice were sacrificed and blood, skin, spleen, as well as intestine samples, were obtained. Assessment score of the skin lesion and serum immunoglobulin E (IgE) level of both IP-PA1 groups were significantly lower than that of the DFE group on days 14 and 21. The serum periostin and thymus and activation-regulated chemokine (TARC) level of IP-PA1-Low group was significantly lower than that of the DFE group on day 31. On histological examination of the skin, hyperplasia of epidermal and dermal layers and infiltration of inflammatory cells were suppressed by IP-PA1 administration. Deposition of periostin was observed in the DFE group skin tissue. Moreover, the CD4(+)/CD8(+) ratio of splenic T-cells increased by IP-PA1 administration. IP-PA1 administration may have an inhibitory effect on AD skin lesions. Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  5. The effect of N-formimidoyl thienamycin, ceftazidime, cefotiam, ceftriaxone and cefotaxime on non-fermentative Gram-negative rods, Aeromonas, Plesiomonas and Enterobacter agglomerans.

    Science.gov (United States)

    von Graevenitz, A; Bucher, C

    1982-01-01

    Thirty-one species (185 strains) of non-fermentative gram-negative rods (excluding Pseudomonas aeruginosa) as well as 45 strains of Aeromonas spp., 15 strains of Plesiomonas shigelloides and 68 strains of Enterobacter agglomerans were tested in microdilution procedures against N-formimidoyl thienamycin, ceftazidime, cefotiam, ceftriaxone and cefotaxime. N-formimidoyl thienamycin was the most effective drug as far as the spectrum of these bacterial groups and potency is concerned; ceftazidime was the second most effective agent. Ceftriaxone and cefotaxime were similar in their activity (against a smaller spectrum), while cefotiam showed little effect. There were occasional differences between MBC and MIC values which were most notable with ceftazidime, cefotiam, ceftriaxone and cefotaxime against E. agglomerans.

  6. Assessment of the relevance of the antibiotic 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine from Pantoea agglomerans biological control strains against bacterial plant pathogens.

    Science.gov (United States)

    Sammer, Ulrike F; Reiher, Katharina; Spiteller, Dieter; Wensing, Annette; Völksch, Beate

    2012-12-01

    The epiphyte Pantoea agglomerans 48b/90 (Pa48b) is a promising biocontrol strain against economically important bacterial pathogens such as Erwinia amylovora. Strain Pa48b produces the broad-spectrum antibiotic 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine (APV) in a temperature-dependent manner. An APV-negative mutant still suppressed the E. amylovora population and fire blight disease symptoms in apple blossom experiments under greenhouse conditions, but was inferior to the Pa48b wild-type indicating the influence of APV in the antagonism. In plant experiments with the soybean pathogen Pseudomonas syringae pv. glycinea both, Pa48b and the APV-negative mutant, successfully suppressed the pathogen. Our results demonstrate that the P. agglomerans strain Pa48b is an efficient biocontrol organism against plant pathogens, and we prove its ability for fast colonization of plant surfaces over a wide temperature range. © 2012 The Authors. Published by Blackwell Publishing Ltd.

  7. Degradation of acephate by Enterobacter asburiae, Bacillus cereus and Pantoea agglomerans isolated from diamondback moth Plutella xylostella (L), a pest of cruciferous crops.

    Science.gov (United States)

    Ramya, Shanivarsanthe Leelesh; Venkatesan, Thiruvengadam; Murthy, Kottilingam Srinivasa; Jalali, Sushil Kumar; Varghese, Abraham

    2016-07-01

    Acephate-degrading bacterial isolates were isolated from the larval gut of diamondback moth Plutella xylostella, a notorious pest of cruciferous crops worldwide that has developed resistance to insecticides. Partial 16S rRNA gene sequencing identified the isolates as Bacillus cereus (PX-B.C.Or), Enterobacter asburiae (PXE), and Pantoae agglomerans (PX-Pt.ag.Jor). All isolates grew on minimal media (MM) in the presence of acephate at 100 and 200 ppm, with maximum growth at 200 ppm. LC-MS analyses of spent medium showed that E. asburiae degraded acephate to methamidophos and O, O-dimethyl phosporamidate and B. cereus O,S-dimethyl to phosphorothioate but P. agglomerans to an unnamed compound. All three isolates used acephate as a source of carbon and energy for growth; however, P. agglomerans used it also as source of sulphur. Strong evidence revealed that the bacterial communities present in the gut of diamondback moth might aid in acephate degradation and play a role in the development of insecticide resistance.

  8. Sepsis temprana en un recién nacido pretérmino por Pantoea agglomerans: informe de caso y revisión de la literatura

    Directory of Open Access Journals (Sweden)

    Eduardo Augusto Gálvez Cuitiva

    2018-01-01

    Full Text Available INTRODUCCIÓN: Pantoea Agglomerans es un bacilo gramnegativo miembro de la familia Enterobacteriaceae, de distribución ubicua que, bajo ciertas circunstancias, puede tener un comportamiento patógeno en el ser humano. Son pocos los casos de infección neonatal no asociados al cuidado de la salud descritos en la literatura; ninguno de ellos reportado en Colombia. PRESENTACIÓN DEL CASO: niña recién nacida, pretérmino, que desde su primer día presentó una inadecuada adaptación neonatal, con dificultad respiratoria, sepsis y un rápido deterioro clínico, lo cual requirió manejo y soporte en la unidad de cuidados intensivos neonatales. Se aisló por hemocultivos P. agglomerans, susceptible al manejo antibiótico instaurado, con una respuesta adecuada, así como una evolución clínica favorable. CONCLUSIÓN: la sepsis neonatal por P. agglomerans es poco frecuente, por lo que su desenlace dependerá de muchos factores: la detección temprana, la asistencia oportuna y el manejo adecuado que se brinde.

  9. Secretion of anti-Plasmodium effector proteins from a natural Pantoea agglomerans isolate by using PelB and HlyA secretion signals.

    Science.gov (United States)

    Bisi, Dawn C; Lampe, David J

    2011-07-01

    The insect-vectored disease malaria is a major world health problem. New control strategies are needed to supplement the current use of insecticides and medications. A genetic approach can be used to inhibit development of malaria parasites (Plasmodium spp.) in the mosquito host. We hypothesized that Pantoea agglomerans, a bacterial symbiont of Anopheles mosquitoes, could be engineered to express and secrete anti-Plasmodium effector proteins, a strategy termed paratransgenesis. To this end, plasmids that include the pelB or hlyA secretion signals from the genes of related species (pectate lyase from Erwinia carotovora and hemolysin A from Escherichia coli, respectively) were created and tested for their efficacy in secreting known anti-Plasmodium effector proteins (SM1, anti-Pbs21, and PLA2) in P. agglomerans and E. coli. P. agglomerans successfully secreted HlyA fusions of anti-Pbs21 and PLA2, and these strains are under evaluation for anti-Plasmodium activity in infected mosquitoes. Varied expression and/or secretion of the effector proteins was observed, suggesting that the individual characteristics of a particular effector may require empirical testing of several secretion signals. Importantly, those strains that secreted efficiently grew as well as wild-type strains under laboratory conditions and, thus, may be expected to be competitive with the native microbiota in the environment of the mosquito midgut.

  10. Secretion of Anti-Plasmodium Effector Proteins from a Natural Pantoea agglomerans Isolate by Using PelB and HlyA Secretion Signals▿

    Science.gov (United States)

    Bisi, Dawn C.; Lampe, David J.

    2011-01-01

    The insect-vectored disease malaria is a major world health problem. New control strategies are needed to supplement the current use of insecticides and medications. A genetic approach can be used to inhibit development of malaria parasites (Plasmodium spp.) in the mosquito host. We hypothesized that Pantoea agglomerans, a bacterial symbiont of Anopheles mosquitoes, could be engineered to express and secrete anti-Plasmodium effector proteins, a strategy termed paratransgenesis. To this end, plasmids that include the pelB or hlyA secretion signals from the genes of related species (pectate lyase from Erwinia carotovora and hemolysin A from Escherichia coli, respectively) were created and tested for their efficacy in secreting known anti-Plasmodium effector proteins (SM1, anti-Pbs21, and PLA2) in P. agglomerans and E. coli. P. agglomerans successfully secreted HlyA fusions of anti-Pbs21 and PLA2, and these strains are under evaluation for anti-Plasmodium activity in infected mosquitoes. Varied expression and/or secretion of the effector proteins was observed, suggesting that the individual characteristics of a particular effector may require empirical testing of several secretion signals. Importantly, those strains that secreted efficiently grew as well as wild-type strains under laboratory conditions and, thus, may be expected to be competitive with the native microbiota in the environment of the mosquito midgut. PMID:21602368

  11. Efecto de diversas actividades físicas y deportivas sobre la autoeficacia de estudiantes universitarios costarricense

    Directory of Open Access Journals (Sweden)

    Campos Salazar, Cinthya

    2013-09-01

    Full Text Available El presente artículo fue realizado con estudiantes universitarios matriculados en el curso de “Actividad Deportiva” de la Universidad de Costa Rica. Los propósitos fueron: (1 comprobar si existe relación significativa entre el nivel de autoeficacia, entendida como la confianza en sí mismo para iniciar y mantenerse en un plan de ejercicio, y la cantidad de actividad física que reporta el estudiantado al inicio del curso; (2 establecer si existe un efecto significativo de las diversas actividades físicas y deportivas en el nivel de autoeficacia, tanto para efectuar ejercicio como para no abandonarlo; y (3 comprobar si los cambios en la autoeficacia son influenciados por el género o el tipo de actividad física realizada (cursos de acondicionamiento físico versus cursos de deportes. Se trabajó con 211 estudiantes matriculados en el III ciclo de 2009, durante el verano. Al estudiantado se le aplicó, antes de iniciar el curso y una vez finalizado, la encuesta de autoeficacia para comportamiento de ejercicio y el Cuestionario Internacional de Actividad Física (IPAQ, con el fin de medir el nivel de actividad física. Los resultados mostraron una asociación positiva entre el nivel de actividad física reportada por el(la estudiante y el nivel de autoeficacia (p<0.001. En cuanto a este último, las mujeres presentaron niveles inferiores que los varones (p<0.001, asimismo el nivel de autoeficacia aumentó significativamente (p<0.05 independientemente del género y del tipo de actividad física. En conclusión, los cursos de actividad deportiva produjeron efectos positivos en la autoeficacia de los participantes, además, los estudiantes más activos presentaron mayores niveles de ella.AbstractThis article was conducted with college students enrolled in the course "Sports Activity" at the University of Costa Rica. The purposes were: (1 to determine whether significant associations existed between levels of self-efficacy, defined as the

  12. Pantoea agglomerans: a marvelous bacterium of evil and good.Part I. Deleterious effects: Dust-borne endotoxins and allergens - focus on cotton dust.

    Science.gov (United States)

    Dutkiewicz, Jacek; Mackiewicz, Barbara; Lemieszek, Marta Kinga; Golec, Marcin; Milanowski, Janusz

    2015-01-01

    The ubiquitous Gram-negative bacterium Pantoea agglomerans (synonyms: Enterobacter agglomerans, Erwinia herbicola) is known both as an epiphytic microbe developing on the surface of plants and as an endophytic organism living inside the plants. The bacterium occurs also abundantly in plant and animal products, in the body of arthropods and other animals, in water, soil, dust and air, and occasionally in humans. From the human viewpoint, the role of this organism is ambiguous, both deleterious and beneficial: on one side it causes disorders in people exposed to inhalation of organic dusts and diseases of crops, and on the other side it produces substances effective in the treatment of cancer and other diseases of humans and animals, suppresses the development of various plant pathogens, promotes plant growth, and appears as a potentially efficient biofertilizer and bioremediator. P. agglomerans was identified as a predominant bacterium on cotton plant grown all over the world, usually as an epiphyte, rarely as pathogen. It is particularly numerous on cotton bract after senescence. During processing of cotton in mills, bacteria and their products are released with cotton dust into air and are inhaled by workers, causing respiratory and general disorders, usually defined as byssinosis. The most adverse substance is endotoxin, a heteropolymer macromolecule present in the outermost part of the cell wall, consisting of lipopolysaccharide (LPS) as a major constituent, phospholipids and protein. The numerous experiments carried out in last quarter of XXth century on laboratory animals and human volunteers supported a convincing evidence that the inhaled endotoxin produced by P. agglomerans causes numerous pathologic effects similar to those elicited by cotton dust, such as influx of free lung cells into airways and activation of alveolar macrophages which secrete mediators (prostaglandins, platelet-activating factor, interleukin-1, tumor necrosis factor) that cause

  13. Pantoea agglomerans: a marvelous bacterium of evil and good.Part I. Deleterious effects: Dust-borne endotoxins and allergens – focus on cotton dust

    Directory of Open Access Journals (Sweden)

    Jacek Dutkiewicz

    2015-12-01

    Full Text Available The ubiquitous Gram-negative bacterium Pantoea agglomerans (synonyms: Enterobacter agglomerans, Erwinia herbicola is known both as an epiphytic microbe developing on the surface of plants and as an endophytic organism living inside the plants. The bacterium occurs also abundantly in plant and animal products, in the body of arthropods and other animals, in water, soil, dust and air, and occasionally in humans. From the human viewpoint, the role of this organism is ambiguous, both deleterious and beneficial: on one side it causes disorders in people exposed to inhalation of organic dusts and diseases of crops, and on the other side it produces substances effective in the treatment of cancer and other diseases of humans and animals, suppresses the development of various plant pathogens, promotes plant growth, and appears as a potentially efficient biofertilizer and bioremediator. P. agglomerans was identified as a predominant bacterium on cotton plant grown all over the world, usually as an epiphyte, rarely as pathogen. It is particularly numerous on cotton bract after senescence. During processing of cotton in mills, bacteria and their products are released with cotton dust into air and are inhaled by workers, causing respiratory and general disorders, usually defined as byssinosis. The most adverse substance is endotoxin, a heteropolymer macromolecule present in the outermost part of the cell wall, consisting of lipopolysaccharide (LPS as a major constituent, phospholipids and protein. The numerous experiments carried out in last quarter of XXth century on laboratory animals and human volunteers supported a convincing evidence that the inhaled endotoxin produced by P. agglomerans causes numerous pathologic effects similar to those elicited by cotton dust, such as influx of free lung cells into airways and activation of alveolar macrophages which secrete mediators (prostaglandins, platelet-activating factor, interleukin-1, tumor necrosis factor

  14. Pantoea agglomerans : a marvelous bacterium of evil and good. Part I. Deleterious effects: Dust-borne endotoxins and allergens – focus on cotton dust

    Directory of Open Access Journals (Sweden)

    Jacek Dutkiewicz

    2015-12-01

    Full Text Available The ubiquitous Gram-negative bacterium Pantoea agglomerans (synonyms: Enterobacter agglomerans , Erwinia herbicola is known both as an epiphytic microbe developing on the surface of plants and as an endophytic organism living inside the plants. The bacterium occurs also abundantly in plant and animal products, in the body of arthropods and other animals, in water, soil, dust and air, and occasionally in humans. From the human viewpoint, the role of this organism is ambiguous, both deleterious and beneficial: on one side it causes disorders in people exposed to inhalation of organic dusts and diseases of crops, and on the other side it produces substances effective in the treatment of cancer and other diseases of humans and animals, suppresses the development of various plant pathogens, promotes plant growth, and appears as a potentially efficient biofertilizer and bioremediator. P. agglomerans was identified as a predominant bacterium on cotton plant grown all over the world, usually as an epiphyte, rarely as pathogen. It is particularly numerous on cotton bract after senescence. During processing of cotton in mills, bacteria and their products are released with cotton dust into air and are inhaled by workers, causing respiratory and general disorders, usually defined as byssinosis. The most adverse substance is endotoxin, a heteropolymer macromolecule present in the outermost part of the cell wall, consisting of lipopolysaccharide (LPS as a major constituent, phospholipids and protein. The numerous experiments carried out in last quarter of XXth century on laboratory animals and human volunteers supported a convincing evidence that the inhaled endotoxin produced by P. agglomerans causes numerous pathologic effects similar to those elicited by cotton dust, such as influx of free lung cells into airways and activation of alveolar macrophages which secrete mediators (prostaglandins, platelet-activating factor, interleukin-1, tumor necrosis factor

  15. Differences between Pseudomonas syringae pv. syringae B728a and Pantoea agglomerans BRT98 in Epiphytic and Endophytic Colonization of Leaves

    Science.gov (United States)

    Sabaratnam, Siva; Beattie, Gwyn A.

    2003-01-01

    The leaf colonization strategies of two bacterial strains were investigated. The foliar pathogen Pseudomonas syringae pv. syringae strain B728a and the nonpathogen Pantoea agglomerans strain BRT98 were marked with a green fluorescent protein, and surface (epiphytic) and subsurface (endophytic) sites of bean and maize leaves in the laboratory and the field were monitored to see if populations of these strains developed. The populations were monitored using both fluorescence microscopy and counts of culturable cells recovered from nonsterilized and surface-sterilized leaves. The P. agglomerans strain exclusively colonized epiphytic sites on the two plant species. Under favorable conditions, the P. agglomerans strain formed aggregates that often extended over multiple epidermal cells. The P. syringae pv. syringae strain established epiphytic and endophytic populations on asymptomatic leaves of the two plant species in the field, with most of the P. syringae pv. syringae B728a cells remaining in epiphytic sites of the maize leaves and an increasing number occupying endophytic sites of the bean leaves in the 15-day monitoring period. The epiphytic P. syringae pv. syringae B728a populations appeared to originate primarily from multiplication in surface sites rather than from the movement of cells from subsurface to surface sites. The endophytic P. syringae pv. syringae B728a populations appeared to originate primarily from inward movement through the stomata, with higher levels of multiplication occurring in bean than in maize. A rainstorm involving a high raindrop momentum was associated with rapid growth of the P. agglomerans strain on both plant species and with rapid growth of both the epiphytic and endophytic populations of the P. syringae pv. syringae strain on bean but not with growth of the P. syringae pv. syringae strain on maize. These results demonstrate that the two bacterial strains employed distinct colonization strategies and that the epiphytic and

  16. Controle biológico de Hypsipyla grandella Zeller (lepidoptera: pyralidae) e novos relatos de ácaros e fungos em mogno (Swietenia macrophylla King) em Brasília/DF

    OpenAIRE

    Castro, Marcelo Tavares de

    2017-01-01

    O mogno (Swietenia macrophylla King) é uma espécie florestal extremamente apreciada pela qualidade de sua madeira, plasticidade silvicultural e beleza ornamental. O seu corte em áreas naturais foi tão abusivo que atualmente é uma espécie protegida e ameaçada de extinção. Apesar disso, o cultivo do mogno tem se estabelecido em diversas partes do mundo, inclusive no Brasil, tanto em plantios comerciais, quanto na arborização de diversas cidades. Porém, um dos principais entraves para o estabele...

  17. Structure of the nifQ gene from Enterobacter agglomerans 333 and its overexpression in Escherichia coli.

    Science.gov (United States)

    Siddavattam, D; Singh, M; Klingmüller, W

    1993-06-01

    The nifQ gene, involved in early stages of iron-molybdenum cofactor (FeMo-co) biosynthesis, was identified downstream of the nifB and nifF genes of Enterobacter agglomerans. This gene was cloned and its nucleotide sequence determined. The amino acid sequence, as deduced from the nucleotide sequence, revealed an accumulation of cysteine amino acid residues at the C-terminal end of the protein. The cysteine cluster showed the following consensus sequence Cys-X4-Cys-X2-Cys-X5-Cys, which is a typical characteristic of metal-binding proteins. Further, the nifQ gene was cloned downstream of strong transcriptional (bacteriophage lambda PLPR) and translational (atpE) signals of the expression vector pCYTEXP1 and expressed as an unfused, soluble protein in Escherichia coli. The molecular mass of 19 kDa, as deduced by SDS-PAGE, is in good agreement with the molecular mass deduced from the nucleotide sequence. The availability of high-level expression clones should facilitate purification of large quantities of the recombinant NifQ protein and elucidation of its properties.

  18. Volatiles production and attractiveness to the Mexican fruit fly of Enterobacter agglomerans isolated from apple maggot and Mexican fruit flies.

    Science.gov (United States)

    Robacker, David C; Lauzon, Carol R; He, Xiaodun

    2004-07-01

    We investigated two strains of uricase (+) Enterobacter agglomerans, one isolated from the apple maggot fly (AMF) and one from the Mexican fruit fly (MFF), for 1) attractiveness to MFF, and 2) production of attractive chemicals. Regarding chemicals demonstrated attractive to the MFF, the MFF bacterial strain produced more 2,5-dimethylpyrazine, 2-phenylethanol, and indole than the AMF strain, whereas the AMF, but not the MFF strain, produced 3-hydroxybutanone. Cell types that predominated in plated subcultures varied from batch to batch resulting in variation in volatiles production, especially by the AMF strain where indole was sometimes a major component of the odor and at other times not detectable. Despite the greater production of attractive chemicals by the MFF strain, the AMF strain was consistently more attractive and the MFF strain was not different from uninoculated control plates. Statistical analyses indicated negative correlations of attractiveness with production of indole, 2,5-dimethylpyrazine, and 2-phenylethanol, and positive correlation with 3-hydroxybutanone. Results support previous findings with the Mexican fruit fly that showed combinations of attractive chemicals sometimes are not attractive.

  19. [Enterobacter agglomerans B1 producing beta-galactosidase with transglycosylation activity: screening, identification, fermentation conditions, and galacto-oligosaccharides synthesis].

    Science.gov (United States)

    Lu, Lili; Xiao, Min; Xu, Xiaodong

    2008-01-01

    Galacto-oligosaccharides (GOS) are promising non-digestible oligosaccharides recognized as prebiotics. Commercial GOS containing galactose as subunit, are synthesized from lactose using the galactosyl-transferase activity of beta-galactosidase. A strain producing beta-galactosidase with transglycosylation activity was screened from the soil. Phenotypic analysis including morphology and physiology characteristics and 16S rDNA sequence analysis were carried out. Based on taxonomy results, the strain was identified as Enterobacter agglomerans B1. Medium and fermentation conditions were optimized by single factor and orthogonal experiments. The enzyme reached 9.7 U/mL in the medium (pH 7.5) containing 1% lactose, 1% yeast extract, and 0.5% peptone when cultured at 25 degrees C for 26 h. Effects of pH, temperature, lactose concentration, and reaction time on transgalactosylation by whole cells were studied. Yield of GOS reached 40.7% in 30% lactose (pH 7.5) at 50 degrees C for 12 h, as analyzed by HPLC and TLC. The results of an MS analysis showed that GOS were composed of di, tri-, and tetrasaccharides.

  20. Effect of Lipopolysaccharide Derived from Pantoea agglomerans on the Phagocytic Activity of Amyloid β by Primary Murine Microglial Cells.

    Science.gov (United States)

    Kobayashi, Yutaro; Inagawa, Hiroyuki; Kohchi, Chie; Okazaki, Katsuichiro; Zhang, Ran; Soma, Gen-Ichiro

    2016-07-01

    Monophosphoryl lipid A, lipopolysaccharide (LPS)-derived Toll-like receptor (TLR) 4 agonist, has been shown to be effective in the prevention of Alzheimer's disease (AD) by enhancing phagocytosis of amyloid β (Aβ) by brain microglia. Our recent study demonstrated that oral administration of LPS derived from Pantoea agglomerans (LPSp) activates peritoneal macrophages and enhances the phagocytic activity via TLR4 signaling pathway; however, the effect of LPSp on Aβ phagocytosis in microglia is still unknown. Primary microglial cells were isolated from adult mouse brain by enzymatic digestion, following myelin removal and magnetic separation of cluster of differentiation (CD) 11b. Phagocytic analysis of the primary microglia was measured by using HiLyte™ Fluor 488-conjugated Aβ1-42 RESULTS: Using our protocols, the average yield of isolated CD11b(+) cells was around 2.2×10(5) cells per brain. CD11b(+)CD45(+)CD39(+) cells were defined here as microglia. The phagocytic activity of Aβ1-42 by the isolated microglia was confirmed. LPSp (10 ng/ml) pre-treatment for 18 h significantly increased Aβ phagocytic activity. The enhancement of Aβ1-42 phagocytosis by LPSp treatment in the primary mouse microglia was demonstrated for the first time. Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

  1. SPM43.1 contributes to acid-resistance of non-symplasmata-forming cells in Pantoea agglomerans YS19.

    Science.gov (United States)

    Li, Qianqian; Miao, Yuxuan; Yi, Ting; Zhou, Jia; Lu, Zhenyue; Feng, Yongjun

    2012-03-01

    Pantoea agglomerans YS19 is a rice endophytic bacterium characterized to form multicellular biofilm-like structures called symplasmata. Phenotypic distinctions between symplasmata-forming cells and planktonic cells are crucial for understanding YS19's survival strategies. In this study, a 43.1 kDa protein SPM43.1 was identified to show significant resistance to the aggregation effect caused by denaturing acidic conditions. MALDI-TOF analysis data indicated that it is a maltose-binding protein homolog while contains sequence homologous to the chaperone protein, ClpB. The purified SPM43.1 protein was detected to exhibit chaperone-like activities at acidic conditions, where its conformation transformed from an ordered to a globally less ordered structure as revealed by circular dichroism spectroscopy, showing a similar property to most chaperone proteins. The expression of SPM43.1 in YS19 is initiated when bacterial cells begin to aggregate, yet its amount in planktonic cells greatly exceeds that in symplasmata-forming cells, suggesting its crucial role to the survival of planktonic cells in experiencing environmental fluctuations. However, the bacterium prefers to form symplasmata, while not to express SPM43.1 proteins, for surviving the artificially set fluctuant (acid here) environments. This study provides valuable information on the life styles and survival strategies of microorganisms that forms multicellular aggregates at specific growth stages.

  2. Lipopolysaccharides Derived from Pantoea agglomerans Can Promote the Phagocytic Activity of Amyloid β in Mouse Microglial Cells.

    Science.gov (United States)

    Kobayashi, Yutaro; Inagawa, Hiroyuki; Kohchi, Chie; Okazaki, Katsuichiro; Zhang, Ran; Kobara, Hideki; Masaki, Tsutomu; Soma, Gen-Ichiro

    2017-07-01

    Recent studies reported that lipopolysaccharide (LPS) exhibits beneficial effects on prevention of immune-related diseases by activating macrophages. We previously demonstrated that pre-treatment with LPS derived from Pantoea agglomerans (LPSp) activated amyloid β (Aβ) phagocytosis in mouse primary microglia. In the present study, we further examined the promotory effect on phagocytosis of phagocytic particles in the C8-B4 microglia cell line. Phagocytic analysis of C8-B4 cells was evaluated using phagocytic particles (latex beads or HiLyte™ Fluor 488-conjugated Aβ 1-42 ). The phagocytic activity of latex beads was dependent on the concentration of beads and incubation time. LPSp, at as low as 100 pg/ml, significantly increased phagocytosis against the beads. In the experiment of Aβ 1-42 phagocytosis, LPSp significantly increased Aβ phagocytic activity. LPSp treatment was confirmed to enhance Aβ 1-42 phagocytosis by mouse microglia. It is suggested that the use of LPSp may be a potential promising candidate for the prevention of Alzheimer's disease. Copyright© 2017, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

  3. Lipopolysaccharide IP-PA1 from Pantoea agglomerans prevents suppression of macrophage function in stress-induced diseases.

    Science.gov (United States)

    Nakata, Kazue; Inagawa, Hiroyuki; Soma, Gen-Ichiro

    2011-07-01

    Chronic psychological stress impairs health and induces various diseases by causing an imbalance in the immune, neuropsychiatric and endocrine systems. The primary reason for the development of stress-induced disease is suppression of macrophage function, which plays a pivotal role in innate immunity. In fact, surgical stress has been shown to exacerbate opportunistic infections by significantly suppressing macrophage function. Conversely, administration of macrophage activating substances before surgery, such as tumor necrosis factor (TNF)-α or Picibanil (OK-432), has been shown to protect against macrophage suppression and the resulting exacerbation of infectious diseases, and against tumor metastasis in the lungs. Thus, if suppression of macrophage function by stress could be safely prevented by use of a macrophage activating substance, the detrimental side effects of stress could be reduced. Recently, we identified a lipopolysaccharide, IP-PA1, derived from Pantoea agglomerans, a symbiotic Gram-negative bacteria found in wheat and other food plants. Oral administration of IP-PA1 demonstrated macrophage activation (priming) and protective effects against infection, allergy and cancer, without any side-effects. In this review, the possibility of using IP-PA1 as a safe, macrophage activating substance for prevention of stress-induced impairments is discussed.

  4. Pantoea agglomerans : a mysterious bacterium of evil and good. Part II. Deleterious effects: Dust-borne endotoxins and allergens – focus on grain dust, other agricultural dusts and wood dust

    Directory of Open Access Journals (Sweden)

    Jacek Dutkiewicz

    2015-12-01

    Full Text Available Pantoea agglomerans , a Gram-negative bacterium developing in a variety of plants as epiphyte or endophyte is particularly common in grain and grain dust, and has been identified by an interdisciplinary group from Lublin, eastern Poland, as a causative agent of work-related diseases associated with exposure to grain dust and other agricultural dusts. The concentration of [i]P. agglomerans[/i] in grain as well as in the settled grain and flour dust was found to be high, ranging from 10 4 –10 8 CFU/g, while in the air polluted with grain or flour dust it ranged from 10 3 –10 5 CFU/m 3 and formed 73.2–96% of the total airborne Gram-negative bacteria. The concentration of P. agglomerans was also relatively high in the air of the facilities processing herbs and other plant materials, while it was lower in animal farms and in wood processing facilities. Pantoea agglomerans produces a biologically-potent endotoxin (cell wall lipopolysaccharide, LPS. The significant part of this endotoxin occurs in dusts in the form of virus-sized globular nanoparticles measuring 10–50 nm that could be described as the ‘endotoxin super-macromolecules’. A highly significant relationship was found (R=0.804, P=0.000927 between the concentration of the viable P. agglomerans in the air of various agricultural and wood industry settings and the concentration of bacterial endotoxin in the air, as assessed by the Limulus test. Although this result may be interfered by the presence of endotoxin produced by other Gram-negative species, it unequivocally suggests the primary role of the P. agglomerans endotoxin as an adverse agent in the agricultural working environment, causing toxic pneumonitis (ODTS. Numerous experiments by the inhalation exposure of animals to various extracts of P. agglomerans strains isolated from grain dust, including endotoxin isolated with trichloroacetic acid (LPS-TCA, endotoxin nanoparticles isolated in sucrose gradient (VECN, and mixture of

  5. Pantoea agglomerans: a mysterious bacterium of evil and good. Part II--Deleterious effects: Dust-borne endotoxins and allergens--focus on grain dust, other agricultural dusts and wood dust.

    Science.gov (United States)

    Dutkiewicz, Jacek; Mackiewicz, Barbara; Lemieszek, Marta Kinga; Golec, Marcin; Skórska, Czesława; Góra-Florek, Anna; Milanowski, Janusz

    2016-01-01

    Pantoea agglomerans, a Gram-negative bacterium developing in a variety of plants as epiphyte or endophyte is particularly common in grain and grain dust, and has been identified by an interdisciplinary group from Lublin, eastern Poland, as a causative agent of work-related diseases associated with exposure to grain dust and other agricultural dusts. The concentration of P. agglomerans in grain as well as in the settled grain and flour dust was found to be high, ranging from 10(4)-10(8) CFU/g, while in the air polluted with grain or flour dust it ranged from 10(3)-10(5) CFU/m(3) and formed 73.2-96% of the total airborne Gram-negative bacteria. The concentration of P. agglomerans was also relatively high in the air of the facilities processing herbs and other plant materials, while it was lower in animal farms and in wood processing facilities. Pantoea agglomerans produces a biologically-potent endotoxin (cell wall lipopolysaccharide, LPS). The significant part of this endotoxin occurs in dusts in the form of virus-sized globular nanoparticles measuring 10-50 nm that could be described as the 'endotoxin super-macromolecules'. A highly significant relationship was found (R=0.804, P=0.000927) between the concentration of the viable P. agglomerans in the air of various agricultural and wood industry settings and the concentration of bacterial endotoxin in the air, as assessed by the Limulus test. Although this result may be interfered by the presence of endotoxin produced by other Gram-negative species, it unequivocally suggests the primary role of the P. agglomerans endotoxin as an adverse agent in the agricultural working environment, causing toxic pneumonitis (ODTS). Numerous experiments by the inhalation exposure of animals to various extracts of P. agglomerans strains isolated from grain dust, including endotoxin isolated with trichloroacetic acid (LPS-TCA), endotoxin nanoparticles isolated in sucrose gradient (VECN), and mixture of proteins and endotoxin obtained

  6. Revealing the inventory of type III effectors in Pantoea agglomerans gall-forming pathovars using draft genome sequences and a machine-learning approach.

    Science.gov (United States)

    Nissan, Gal; Gershovits, Michael; Morozov, Michael; Chalupowicz, Laura; Sessa, Guido; Manulis-Sasson, Shulamit; Barash, Isaac; Pupko, Tal

    2018-02-01

    Pantoea agglomerans, a widespread epiphytic bacterium, has evolved into a hypersensitive response and pathogenicity (hrp)-dependent and host-specific gall-forming pathogen by the acquisition of a pathogenicity plasmid containing a type III secretion system (T3SS) and its effectors (T3Es). Pantoea agglomerans pv. betae (Pab) elicits galls on beet (Beta vulgaris) and gypsophila (Gypsophila paniculata), whereas P. agglomerans pv. gypsophilae (Pag) incites galls on gypsophila and a hypersensitive response (HR) on beet. Draft genome sequences were generated and employed in combination with a machine-learning approach and a translocation assay into beet roots to identify the pools of T3Es in the two pathovars. The genomes of the sequenced Pab4188 and Pag824-1 strains have a similar size (∼5 MB) and GC content (∼55%). Mutational analysis revealed that, in Pab4188, eight T3Es (HsvB, HsvG, PseB, DspA/E, HopAY1, HopX2, HopAF1 and HrpK) contribute to pathogenicity on beet and gypsophila. In Pag824-1, nine T3Es (HsvG, HsvB, PthG, DspA/E, HopAY1, HopD1, HopX2, HopAF1 and HrpK) contribute to pathogenicity on gypsophila, whereas the PthG effector triggers HR on beet. HsvB, HsvG, PthG and PseB appear to endow pathovar specificities to Pab and Pag, and no homologous T3Es were identified for these proteins in other phytopathogenic bacteria. Conversely, the remaining T3Es contribute to the virulence of both pathovars, and homologous T3Es were found in other phytopathogenic bacteria. Remarkably, HsvG and HsvB, which act as host-specific transcription factors, displayed the largest contribution to disease development. © 2016 BSPP AND JOHN WILEY & SONS LTD.

  7. Draft Genome Assemblies of Enterobacter aerogenes CDC 6003-71, Enterobacter cloacae CDC 442-68, and Pantoea agglomerans UA 0804-01.

    Science.gov (United States)

    Minogue, T D; Daligault, H E; Davenport, K W; Bishop-Lilly, K A; Bruce, D C; Chain, P S; Coyne, S R; Chertkov, O; Freitas, T; Frey, K G; Jaissle, J; Koroleva, G I; Ladner, J T; Palacios, G F; Redden, C L; Xu, Y; Johnson, S L

    2014-10-23

    The Enterobacteriaceae are environmental and enteric microbes. We sequenced the genomes of two Enterobacter reference strains, E. aerogenes CDC 6003-71 and E. cloacae CDC 442-68, as well as one near neighbor used as an exclusionary reference for diagnostics, Pantoea agglomerans CDC UA0804-01. The genome sizes range from 4.72 to 5.55 Mbp and have G+C contents from 54.6 to 55.1%. Copyright © 2014 Minogue et al.

  8. Epidemiologia molecular de um surto de bacteriemia por Enterobacter cloacae e Enterobacter agglomerans ocorrido na região de Campinas, São Paulo, Brasil

    OpenAIRE

    GONÇALVES, Célia R.; VAZ, Tania M.I.; LEITE, Daniela; PISANI, Beatriz; SIMÕES, Marise; PRANDI, Maria Angela M.; ROCHA, Marilu M.M.; CESAR, Paulo C.; TRABASSO, Plinio; NOWAKONSKI, Angela von; IRINO, Kinue

    2000-01-01

    A total of 73 isolates (57 Enterobacter cloacae and 16 Enterobacter agglomerans), recovered during an outbreak of bacteremia in the Campinas area, São Paulo, Brazil, were studied. Of these isolates, 61 were from parenteral nutrition solutions, 9 from blood cultures, 2 from a sealed bottle of parenteral nutrition solution, and one was of unknown origin. Of the 57 E. cloacae isolates, 54 were biotype 26, two were biotype 66 and one was non-typable. Of 39 E. cloacae isolates submitted to ribotyp...

  9. Draft Genome Sequence ofPantoea agglomeransJM1, a Strain Isolated from Soil Polluted by Industrial Production of Beta-Lactam Antibiotics That Exhibits Valacyclovir-Like Hydrolase Activity.

    Science.gov (United States)

    Zahradník, Jiří; Plačková, Martina; Palyzová, Andrea; Marešová, Helena; Kyslíková, Eva; Kyslík, Pavel

    2017-09-21

    Strain Pantoea agglomerans JM1 was isolated from the soil of a microbiome that had been exposed to polluting pharmaceuticals. The bacterium exhibited enzymatic activities useful for the biotransformation of beta-lactams. The genome of the strain was assembled and described, and the gene encoding valacyclovir-like hydrolase was identified. Copyright © 2017 Zahradník et al.

  10. Partial Purification and Characterization of an Inducible Indole-3-Acetyl-L-Aspartic Acid Hydrolase from Enterobacter agglomerans.

    Science.gov (United States)

    Chou, J. C.; Kuleck, G. A.; Cohen, J. D.; Mulbry, W. W.

    1996-11-01

    Indole-3-acetyl-amino acid conjugate hydrolases are believed to be important in the regulation of indole-3-acetic acid (IAA) metabolism in plants and therefore have potential uses for the alteration of plant IAA metabolism. To isolate bacterial strains exhibiting significant indole-3-acetyl-aspartate (IAA-Asp) hydrolase activity, a sewage sludge inoculation was cultured under conditions in which IAA-Asp served as the sole source of carbon and nitrogen. One isolate, Enterobacter agglomerans, showed hydrolase activity inducible by IAA-L-Asp or N-acetyl-L-Asp but not by IAA, (NH4)2SO4, urea, or indoleacetamide. Among a total of 17 IAA conjugates tested as potential substrates, the enzyme had an exclusively high substrate specificity for IAA-L-Asp. Substrate concentration curves and Lineweaver-Burk plots of the kinetic data showed a Michaelis constant value for IAA-L-Asp of 13.5 mM. The optimal pH for this enzyme was between 8.0 and 8.5. In extraction buffer containing 0.8 mM Mg2+ the hydrolase activity was inhibited to 80% by 1 mM dithiothreitol and to 60% by 1 mm CuSO4; the activity was increased by 40% with 1 mM MnSO4. However, in extraction buffer with no trace elements, the hydrolase activity was inhibited to 50% by either 1 mM dithiothreitol or 1% Triton X-100 (Sigma). These results suggest that disulfide bonding might be essential for enzyme activity. Purification of the hydrolase by hydroxyapatite and TSK-phenyl (HP-Genenchem, South San Francisco, CA) preparative high-performance liquid chromatography yielded a major 45-kD polypeptide as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

  11. Partial purification and characterization of an inducible indole-3-acetyl-L-aspartic acid hydrolase from Enterobacter agglomerans

    Energy Technology Data Exchange (ETDEWEB)

    Chou, Jyh-Ching [Department of Agriculture, Beltsville, MD (United States)]|[Univ. of Maryland, College Park, MD (United States); Cohen, J.D.; Mulbry, W.W. [Department of Agriculture, Beltsville, MD (United States)] [and others

    1996-11-01

    Indole-3-acetyl-amino acid conjugate hydrolases are believed to be important in the regulation of indole-3-acetic acid (IAA) metabolism in plants and therefore have potential uses for the alteration of plant IAA metabolism. To isolate bacterial strains exhibiting significant indole-3-acetyl-aspartate (IAA-Asp) hydrolase activity, a sewage sludge inoculation was cultured under conditions in which IAA-Asp served as the sole source of carbon and nitrogen. One isolate, Enterobacter agglomerans, showed hydrolase activity inducible by IAA-L-Asp or N-acetyl-L-Asp but not by IAA, (NH{sub 4}){sub 2}SO{sub 4}, urea, or indoleacetamide. Among a total of 17 IAA conjugates tested as potential substrates, the enzyme had an exclusively high substrate specificity for IAA-L-Asp of 13.5 mM. The optimal pH for this enzyme was between 8.0 and 8.5. In extraction buffer containing 0.8 mM Mg{sup 2+} the hydrolase activity was inhibited to 80% by 1 mM dithiothreitol and to 60% by 1 mm CuSO{sub 4}; the activity was increased by 40% with 1mM MnSO{sub 4}. However, in extraction buffer with no trace elements, the hydrolase activity was inhibited to 50% by either 1 mM dithiothreitol or 1% Triton X-100 (Sigma). These results suggest that disulfide bonding might be essential for enzyme activity. Purification of the hydrolase by hydroxyapatite and TSK-phenyl (HP-Genenchem, South San Francisco, CA) preparative high-performance liquid chromatography yielded a major 45-kD polypeptide as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 45 refs., 5 figs., 3 tabs.

  12. Directed evolution of a family 26 glycoside hydrolase: endo-β-1, 4-mannanase from Pantoea agglomerans A021.

    Science.gov (United States)

    Wang, Jing; Zhang, Qingye; Huang, Zongqing; Liu, Ziduo

    2013-09-10

    Pantoea agglomerans endo-β-1, 4-mannanase (Man26P) hydrolyzes hemicellulose by cleaving the internal bonds in the mannan-based chains. In order to obtain improved enzymes and understand the structure-function relationship better, Man26P was engineered using a modified DNA shuffling method and a 96-well plate high-throughput screening technology. Compared to the wild-type enzyme, two mutants Gly267Ser and His134Arg/Phe141Leu with 1.14- and 3.30-fold increased catalytic efficiency (k(cat)/K(m)) toward locust bean gum (LBG) were obtained from the mutant library containing 19,700 clones. Combined with site-directed mutagenesis, more substitutions were introduced into the 134 site, among which, His134Arg and His134Lys exhibited 2.81- and 2.75-fold increased catalytic efficiency versus the wild-type enzyme. And with an increase in the pK(a) values of the side chains of the mutated residues at the 134 site, a decrease was observed in their K(m) values against LBG. The molecular modeling and the docking analysis of the substrate suggest that the enhancement of polar contacts or positive charges at the 134 site contribute to the interaction of the enzyme with the substrate. Furthermore, the nature of the residue at the 267 site could also significantly affect the enzyme activity by regulating substrate binding affinity and product release. The study provides useful information for the directed evolution of mannanases. Copyright © 2013 Elsevier B.V. All rights reserved.

  13. The gene for indole-3-acetyl-L-aspartic acid hydrolase from Enterobacter agglomerans: molecular cloning, nucleotide sequence, and expression in Escherichia coli.

    Science.gov (United States)

    Chou, J C; Mulbry, W W; Cohen, J D

    1998-08-01

    A 5.5-kb DNA fragment containing the indole-3-acetyl-aspartic acid (IAA-asp) hydrolase gene (iaaspH) was isolated from Enterobacter agglomerans strain GK12 using a hybridization probe based on the N-terminal amino acid sequence of the protein. The DNA sequence of a 2.4-kb region of this fragment was determined and revealed a 1311-nucleotide ORF large enough to encode the 45-kDa IAA-asp hydrolase. A 1.5-kb DNA fragment containing iaaspH was subcloned into the Escherichia coli expression plasmid pTTQ8 to yield plasmid pJCC2. Extracts of IPTG-induced E. coli cultures containing the pJCC2 recombinant plasmid showed IAA-asp hydrolase levels 5 to 10-fold higher than those in E. agglomerans extracts. Homology searches revealed that the IAA-asp hydrolase was similar to a variety of amidohydrolases. In addition, IAA-asp hydrolase showed 70% sequence identity to a putative thermostable carboxypeptidase of E. coli.

  14. Identification and characterization of the nifH and nifJ promoter regions located on the nif-plasmid pEA3 of Enterobacter agglomerans 333.

    Science.gov (United States)

    Kreutzer, R; Singh, M; Klingmüller, W

    1989-05-15

    Small restriction fragments of the plasmid-borne Enterobacter agglomerans 333 nif region were cloned into a promoter probe plasmid as transcriptional fusions with the lacZ gene. Identification of NifA-dependent promoters was accomplished by using a compatible plasmid which constitutively expresses the Klebsiella pneumoniae nifA gene. beta-Galactosidase assays showed strong activation of the cloned E. agglomerans promoters in Escherichia coli by the heterologous K. pneumoniae nifA gene product. The positions of the promoter fragments on the corresponding restriction map were determined by Southern hybridization. As confirmed by sequencing data, the nifH and nifJ promoters are situated at opposite end-points of the nif gene group and their -24 to -12 nucleotide sequences are similar to the consensus sequence of NtrA-dependent promoters. Also, typical NifA-binding motifs are present in both promoters. The agreement of the promoter proximal regions of nifH and nifJ with the corresponding K pneumoniae sequences is about 80%. Also the upstream regions of these genes are in agreement to some extent.

  15. The type III effector HsvG of the gall-forming Pantoea agglomerans mediates expression of the host gene HSVGT.

    Science.gov (United States)

    Nissan, Gal; Manulis-Sasson, Shulamit; Chalupowicz, Laura; Teper, Doron; Yeheskel, Adva; Pasmanik-Chor, Metsada; Sessa, Guido; Barash, Isaac

    2012-02-01

    The type III effector HsvG of the gall-forming Pantoea agglomerans pv. gypsophilae is a DNA-binding protein that is imported to the host nucleus and involved in host specificity. The DNA-binding region of HsvG was delineated to 266 amino acids located within a secondary structure region near the N-terminus of the protein but did not display any homology to canonical DNA-binding motifs. A binding site selection procedure was used to isolate a target gene of HsvG, named HSVGT, in Gypsophila paniculata. HSVGT is a predicted acidic protein of the DnaJ family with 244 amino acids. It harbors characteristic conserved motifs of a eukaryotic transcription factor, including a bipartite nuclear localization signal, zinc finger, and leucine zipper DNA-binding motifs. Quantitative real-time polymerase chain reaction analysis demonstrated that HSVGT transcription is specifically induced in planta within 2 h after inoculation with the wild-type P. agglomerans pv. gypsophilae compared with the hsvG mutant. Induction of HSVGT reached a peak of sixfold at 4 h after inoculation and progressively declined thereafter. Gel-shift assay demonstrated that HsvG binds to the HSVGT promoter, indicating that HSVGT is a direct target of HsvG. Our results support the hypothesis that HsvG functions as a transcription factor in gypsophila.

  16. Isolation of Bioactive Phenazine-1-Carboxamide from the Soil Bacterium Pantoea agglomerans and Study of Its Anticancer Potency on Different Cancer Cell Lines.

    Science.gov (United States)

    Ali, Hayssam M; El-Shikh, Mohamed S; Salem, Mohamed Z M; M, Muzaheed

    2016-09-01

    The study was designed to investigate the anticancer effect of phenazine-1-carboxamide (PCN) isolated from the bacterium Pantoea agglomerans naturally present in soil. PCN showed cytotoxicity in a dose-dependent manner, and inhibitory concentrations on the cancer cell lines A549, HeLa, and SW480 were between 32 and 40 μM. Significantly increased concentrations of lactate dehydrogenase were found with increasing concentrations of PCN, which resulted in increased destruction of the cancer cell membrane. A significantly increased p53 level was accompanied by the increased production of cytochrome c protein in all cancer cell lines studied. This condition in cells leads to the overexpression of caspase 3 and Bcl-2 family proteins. Upregulation and downregulation of proapoptotic and antiproapoptotic proteins were analyzed for their messenger RNA and protein expression. The activation of caspases and their cleavage compounds paves the way for the complete apoptosis process in cancer cells. We conclude that P. agglomerans-derived PCN acts as an effective anticancer drug or compound.

  17. Screening of freeze-dried protective agents for the formulation of biocontrol strains, Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40.

    Science.gov (United States)

    Zhan, Y; Xu, Q; Yang, M-M; Yang, H-T; Liu, H-X; Wang, Y-P; Guo, J-H

    2012-01-01

    The effects of different freeze-drying protective agents on the viabilities of biocontrol strains Bacillus cereus AR156, Burkholderia vietnamiensis B418 and Pantoea agglomerans 2Re40 were investigated. Several concentrations of protective and rehydration media were tested to improve the survival of biocontrol agents after freeze-drying. The subsequent survival rates during storage and rehydration media of freeze-dried biocontrol strains were also examined. The results indicated that cellobiose (5%) and d-galactose (5%) gave maximum viability of strains Bu. vietnamiensis B418 and P. agglomerans 2Re40 (98 and 54·3% respectively) while the perfect one (100%) of strain B. cereus AR156 was obtained with sucrose (5%) during freeze-drying, and the highest survival of the three strains was reached when they were rehydrated with 10% nonfat skim milk. In the following storage, the survival rates showed that B. cereus AR156 could still reach 50% after 12 months. This study showed that freeze-drying could be used to stabilize cells of these three biocontrol strains. Further studies should focus on the scale-up possibilities and formulation development. © Nanjing Agricultural University. Letters in Applied Microbiology © 2011 The Society for Applied Microbiology.

  18. Successful treatment of sepsis due to Pantoea agglomerans by polymyxin B-immobilized fiber column direct hemoperfusion therapy in a small cell lung carcinoma patient.

    Science.gov (United States)

    Tsubouchi, Hironobu; Matsumoto, Nobuhiro; Yanagi, Shigehisa; Arimura, Yasuji; Nakazato, Masamitsu

    2016-01-01

    Sepsis is a life-threatening condition caused by the inflammatory response to invading organisms. Polymyxin B-immobilized fiber column direct hemoperfusion (PMX-DHP) is used to reduce blood endotoxin levels and modulate circulating inflammatory cytokine levels in sepsis patients. Here we report that severe sepsis caused by an infection of the gram-negative bacterium Pantoea agglomerans in a patient with small cell lung carcinoma was treated successfully with antibiotics and PMX-DHP. The patient, a 49-year-old Japanese male smoker whose condition was complicated with hyponatremia due to SIADH (syndrome of inappropriate secretion of antidiuretic hormone), rapidly developed sepsis and disseminated intravascular coagulation (DIC) after the administration of cisplatin and irinotecan. Despite initial antibiotics therapy, severe host responses including hypotension, high body temperature and tachycardia were noted. We initiated PMX-DHP, and the patient's Sequential Organ Failure Assessment score was greatly reduced and his DIC improved immediately. To our knowledge, this is the first reported case of PMX-DHP therapy for severe sepsis caused by P. agglomerans infection. Although the efficacy of PMX-DHP in sepsis is not well defined, PMX-DHP therapy should be considered in cases of sepsis from gram-negative infections.

  19. CARNAVAL EM ANDAMENTO: HIBRIDISMO CULTURAL NO CONTEXTO CARNAVALESCO DE BAGÉ RS

    Directory of Open Access Journals (Sweden)

    Rafael Rosa da Silva

    2017-05-01

    Full Text Available Diversas são as discussões e os estudos em torno do carnaval no Brasil, talvez devido às diversas manifestações existentes nesse período e que variam de um estado para o outro, ou mesmo por ser um campo de estudo que traz em si uma pluralidade de aspectos que podem ser analisados em diversas áreas do conhecimento. A respeito do carnaval no Rio Grande Do Sul, existem poucos trabalhos, principalmente no que diz respeito ao carnaval interiorano. Sendo assim, este trabalho procura discutir acerca dos processos de transformação que o samba, na esfera do Bloco Burlesco Brasa Viva, vem sofrendo no carnaval de rua de Bagé RS. Trazer o samba como um processo em constante mudança não só na esfera carnavalesca, mas também no dia a dia de uma parcela da população brasileira é também poder entender um gênero musical que sofre um processo de hibridismo cultural. Trabalhando com bibliografias acerca da temática do carnaval, atrelada ao contexto de inserção do Bloco Burlesco Brasa Viva, percebemos que ao longo dos anos o samba foi sofrendo um processo hibridização, acompanhado por diversas transformações que acabaram padronizando o samba no carnaval de rua de Bagé RS.

  20. Diversas miradas: democracia del amor

    Directory of Open Access Journals (Sweden)

    Miguel López Melero

    2012-01-01

    Full Text Available Al hacer una presentación de esta monografía sobre Amor y Educación lo primero que deseamos destacar es que todos los autores que han colaborado en la misma coinciden en señalar que la educación es un acto amoroso que nos capacita para respetar al otro u otra como legítimo otro u otra en la convivencia. Sin aceptación del otro o la otra en la convivencia no hay educación, pero tampoco la hay sin la aceptación de sí mismo. La condición fundamental y básica para que el ser humano se desarrolle como un ser que aprende a pensar, que aprende a comunicarse, que aprende a sentir y que aprende a actuar, es el amor. El amar surge en el momento que abrimos un espacio a los demás, un espacio a las relaciones con otros u otras, porque supone ver y oír al otro sin prejuicios, sin expectativas, pero para que esto ocurra hay que estar dispuesto a hacerlo, hay que estar dispuesto a quererlo. Una vida sin amor no tiene sentido de ser vivida. Los seres humanos somos hijos del amor. Lo que nos hace seres humanos, según este punto de vista, es nuestro vivir como seres cooperativos y amorosos, con conciencia de sí mismo y con conciencia social, en el respeto por sí mismo y por los otros. Pero para ello –subraya Maturana (1997– debemos abandonar el discurso patriarcal de la lucha y la guerra, y volcarnos en el vivir matrístico del conocimiento de la naturaleza, del respeto y la colaboración en la creación de un mundo que admite el error y puede corregirlo. Una educación que nos lleve a actuar en la conservación de la naturaleza, a entenderla para vivir con ella y en ella sin pretender dominarla, una educación que nos permita vivir en la responsabilidad individual y social que aleja el abuso y trae consigo la colaboración en la creación de un proyecto armonioso entre la naturaleza y el ser humano. Pero sólo si se vive en la biología del amor el individuo desarrolla el respeto a sí mismo y a los demás, así como una conciencia social. De acuerdo con esta teoría, compete al profesorado crear las condiciones necesarias para que el alumnado viva en la biología del amor educándose mutuamente, donde amor y conocimiento no son dos cosas alternativas sino que el amor es el fundamento de la vida humana y el conocimiento sólo un instrumento de la misma (MATURANA, 1994a. Lo más importante es que la educación sea capaz de crear las condiciones que permita a cada cual llegar a ser un ciudadano o una ciudadana culta, autónoma, responsable y, sobre todo, feliz. Por eso necesitamos una escuela pública apasionada por el conocimiento y por la vida (verdad, por el amor al otro u otra como legítimo otro u otra (bondad y por saber vivir en el respeto a la diversidad como valor (belleza. Solemos decir que vivimos en la sociedad del conocimiento y de la incertidumbre y que necesitamos de docentes competentes y comprometidos moralmente con la educación. Profesionales que tengan claro qué tipo de ciudadanía necesitamos y cómo debemos formar a dicha ciudadanía. Nuestro pensamiento es que este compromiso con la educación sólo se podrá lograr si nuestro modelo educativo, además de posibilitar una ciudadanía competente intelectualmente, consiga un buen ciudadano o una buena ciudadana donde el amor al otro u otra como legítimo otro u otra en la convivencia sea el epicentro de su pensamiento y de sus acciones. No sólo necesitamos formar a la ciudadanía como personas intelectualmente cultas, sino moralmente buenas personas. En este sentido las propuestas de los diversos autores apuntan hacia un proyecto moral de educación que propicie una ciudadanía pensante, crítica y culta que sea capaz de problematizar la realidad más cercana. Una ciudadanía libre y responsable capaz de guiar éticamente sus comportamientos y sepa tomar decisiones y asumir la responsabilidades de sus acciones, incluso, en situaciones complejas.

  1. Morphological identification of the Soprano Pipistrelle (<em>Pipistrellus pygmaeusem> Leach, 1825 in Croatia.

    Directory of Open Access Journals (Sweden)

    Igor Pavlinić

    2008-07-01

    presunta diversa lunghezza della seconda e terza falange del terzo dito è invece risultata inefficace per una corretta discriminazione tra <em>P. pipistrellusem> e <em>P. pygmaeusem> in Croazia.

  2. Isolamento de enterobactérias em Alphitobius diaperinus e na cama de aviários no oeste do estado do Paraná, Brasil

    Directory of Open Access Journals (Sweden)

    Chernaki-Leffer AM

    2002-01-01

    Full Text Available O objetivo deste trabalho foi realizar o isolamento de bactérias da família Enterobacteriaceae em adultos de A. diaperinus, buscando uma correlação entre as bactérias presentes no inseto e na cama, em aviários para produção de frangos de corte no oeste do Paraná, Brasil. No primeiro experimento, insetos adultos foram coletados em 14 granjas. No segundo experimento, foram coletados insetos e material da cama de 12 diferentes aviários. Os adultos foram anestesiados com éter, macerados em solução salina e o material da cama colhido por "swab" de arrasto. O enriquecimento não seletivo foi feito com caldo BHI e o seletivo com Rappaport-Vassiliadis e Tetrationato. Os meios de cultivo para plaqueamento foram o ágar MacConkey, ágar Salmonella-Shigella e ágar verde-brilhante. As enterobactérias isoladas em adultos de A. diaperinus foram: Proteus Vulgaris, P. mirabilis, Escherichia Coli, Enterobacter spp., E. agglomerans, E. gergoviae, E. sakasakii, Citrobacter Diversus e Klebsiella Pneumoniae, enquanto que na cama foram encontrados Proteus Vulgaris, P. mirabilis, Escherichia Coli, Enterobacter Agglomerans. Não foram isoladas Salmonella spp. do inseto nem da cama e P. vulgaris foi a predominante. E. coli foi freqüente nas granjas, tanto na cama como nos insetos e contribuem na disseminação da colibacilose em aviários.

  3. Estratégia de comercialização em mercados derivativos: cálculo de base e risco de base do boi gordo em diversas localidades do Brasil

    Directory of Open Access Journals (Sweden)

    Vagner Rosalem

    2008-12-01

    Full Text Available This paper had as it’s main objective to evaluate the feedback that cattlemen, exporters and other actors of the fed cattle market of São José do Rio Preto and Barrtos/SP; Northwester Paraná. Minas Gerais’ triangle, Goiânia/GO and Cuiabá/MT; could obtain from future hedge strategies from September 2000 to November 2003. Such research is worth once it´s new that derivative market has been increasingly adopted due to it’s possibilities of use with hedge prices. The primary data about prices of fed cattle weight were obtained from the Applied Economy Advanced Studies’ Center (CEPEA – ESALQ/USP and future prices of fed cattle weight were obtained from Brazilian Mercantile & Futures Exchange (BM&FBovespa S.A.. It can be said, from the various values of basis and basis risk, that each of the regions has it´s own commerce features. However, it was noticed that the more favorable months to hedge selling strategies were April, October and December, while May and July could be pointed as the best mouths to buying hedge strategies

  4. Revisión bibliográfico analítica acerca de las diversas teorías y programas de intervención del autismo infantil

    Directory of Open Access Journals (Sweden)

    Moisés Mebarak

    2009-01-01

    Full Text Available Este artículo tiene como objetivo realizar una revisión bibliográfica acerca del autismo infantil. Se hace énfasis en las diversas teorías e hipótesis psicológicas que subyacen a este singular trastorno y se complementa la información acerca de las diversas técnicas de intervención que a nivel interdisciplinario se vienen manejando, con el fin de mejorar la calidad de vida de los afectados. El autismo infantil presenta una gran heterogeneidad en sus manifestaciones sintomáticas, por lo que es posible confundirlo a nivel de diagnóstico diferencial. Se han encontrado contradicciones en los resultados de algunas investigaciones en relación con las diversas técnicas de intervención con las que se viene tratando actualmente el trastorno.

  5. Uncoupled glycerol distribution as the origin of the accumulation of 3-hydroxypropionaldehyde during the fermentation of glycerol by enterobacter agglomerans CNCM 1210

    Science.gov (United States)

    Barbirato; Soucaille; Camarasa; Bories

    1998-04-05

    Batch fermentation of glycerol to 1,3-propanediol (1,3PPD) by Enterobacter agglomerans CNCM 1210 showed the lethal accumulation of 3-hydroxypropionaldehyde (3-HPA) when performed under initial substrate content higher than 40 g/L. Assigned to the inhibition by the NAD/NADH ratio of the 3-HPA converting enzyme: 1,3PPD dehydrogenase, intracellular assays were conducted in an attempt to identify the metabolic mechanisms involved in the increase of that ratio. An overflow metabolism through the 1,3PPD formation pathway was established, while a catabolic limitation in the oxidative branch at the level of glyceraldehyde-3-phosphate dehydrogenase occurred. Uncoupled activities of synthesis and consumption of reducing equivalents are thus suspected to provoke the increase of the NAD/NADH ratio and the subsequent accumulation of 3-HPA. Copyright 1998 John Wiley & Sons, Inc.

  6. Sensitivity to pH, product inhibition, and inhibition by NAD+ of 1,3-propanediol dehydrogenase purified from Enterobacter agglomerans CNCM 1210.

    Science.gov (United States)

    Barbirato, F; Larguier, A; Conte, T; Astruc, S; Bories, A

    1997-08-01

    Because of its key role in the metabolism of glycerol during fermentation, 1,3-propanediol dehydrogenase (EC 1.1.1.202) of Enterobacter agglomerans CNCM 1210 was purified to homogeneity and studied with respect to its sensitivity to pH and to nucleotide and 1,3-propanediol concentrations. Enzyme activity was optimal at pH 7.8. The enzyme was competitively inhibited by NAD+ (Ki of 0.29 mM), and 1,3-propanediol exerted a strong inhibitory effect according to a mixed-type inhibition with a Ki of 13.7 mM and an a-factor of 9.0. It is proposed that these dehydrogenase properties be extended to the dehydrogenases of Citrobacter freundii and Klebsiella pneumoniae, which exhibited numerous similar physical properties.

  7. Efeitos da dentina sobre o pH e atividade antimicrobiana de diversas formulações com hidróxido de cálcio

    Directory of Open Access Journals (Sweden)

    Marina Oliveira Gonçalves Galoza

    Full Text Available Objetivo Avaliar o efeito da dentina sobre o pH e a atividade antimicrobiana de formulações com hidróxido de cálcio. Material e método O pH e a atividade antimicrobiana das formulações (G1- polietilenoglicol, G2- glicerina e paramonoclorofenol canforado ou G3- clorexidina foram analisados nas formulações puras (G1A, G2A e G3A ou acrescidas de dentina em pó (G1B, G2B e G3B. Tubos de polietileno foram preenchidos com uma das formulações e imersos em água destilada. O pH foi analisado após 24 horas, 7, 14 e 21 dias. A atividade antimicrobiana foi avaliada sobre Enterococcus faecalis (ATCC 29212, através do teste de difusão em ágar. Os resultados foram submetidos aos testes de ANOVA e Tukey (p=0,05. Resultado A dentina não exerceu efeito sobre o pH das formulações, exceto ocasionando a redução em G1, no período de 24 horas (p0,05. Quando comparados entre os grupos, em 24 horas e 7 dias, G1A e G1B apresentaram menor pH (p0,05, assim como entre G3B e G1A (p>0,05. Em 21 dias, todas as formulações demonstram semelhante pH (p>0,05. Os resultados em ordem decrescente da atividade antimicrobiana foram G2A=G2B>G3A=G3B>G1A=G1B (p<0,05. Conclusão A dentina não interferiu no pH e na atividade antimicrobiana das diferentes formulações com hidróxido de cálcio, exceto na associação com o polietilenoglicol, em que ocasionou a redução do pH apenas no período de 24 horas. A formulação com paramonoclorofenol canforado apresentou maior atividade antimicrobiana, seguida da clorexidina e do polietilenoglicol.

  8. Atomic resolution structure of EhpR: phenazine resistance in Enterobacter agglomerans Eh1087 follows principles of bleomycin/mitomycin C resistance in other bacteria.

    Science.gov (United States)

    Yu, Shen; Vit, Allegra; Devenish, Sean; Mahanty, H Khris; Itzen, Aymelt; Goody, Roger S; Blankenfeldt, Wulf

    2011-08-17

    The phenazines are redox-active secondary metabolites that a large number of bacterial strains produce and excrete into the environment. They possess antibiotic activity owing to the fact that they can reduce molecular oxygen to toxic reactive oxygen species. In order to take advantage of this activity, phenazine producers need to protect themselves against phenazine toxicity. Whereas it is believed that phenazine-producing pseudomonads possess highly active superoxide dismutases and catalases, it has recently been found that the plant-colonizing bacterium Enterobacter agglomerans expresses a small gene ehpR to render itself resistant towards D-alanyl-griseoluteic acid, the phenazine antibiotic produced by this strain. To understand the resistance mechanism installed by EhpR we have determined its crystal structure in the apo form at 2.15 Å resolution and in complex with griseoluteic acid at 1.01 Å, respectively. While EhpR shares a common fold with glyoxalase-I/bleomycin resistance proteins, the ligand binding site does not contain residues that some related proteins employ to chemically alter their substrates. Binding of the antibiotic is mediated by π-stacking interactions of the aromatic moiety with the side chains of aromatic amino acids and by a few polar interactions. The dissociation constant KD between EhpR and griseoluteic acid was quantified as 244 ± 45 μM by microscale thermophoresis measurements. The data accumulated here suggest that EhpR confers resistance by binding D-alanyl-griseoluteic acid and acting as a chaperone involved in exporting the antibiotic rather than by altering it chemically. It is tempting to speculate that EhpR acts in concert with EhpJ, a transport protein of the major facilitator superfamily that is also encoded in the phenazine biosynthesis operon of E. agglomerans. The low affinity of EhpR for griseoluteic acid may be required for its physiological function.

  9. Acid and base hydrolysis of lipid A from Enterobacter agglomerans as monitored by electrospray ionization mass spectrometry: pertinence to detoxification mechanisms.

    Science.gov (United States)

    Wang, Y; Cole, R B

    1996-02-01

    Lipopolysaccharides (LPS), which are endotoxins found in the cell wall of Gram-negative bacteria, are common components of organic dusts that cause or contribute to symptoms associated with organic dust diseases. The lipid A subgroup within LPS is believed to be responsible for the toxicity. Acid and base treatments, which can be effective detoxification methods, were performed on lipid A from Enterobacter agglomerans (EA), a bacterium commonly found in field cotton. Negative-ion electrospray ionization mass spectrometry was employed to characterize the post-treatment structural changes to lipid A. Acid treatment (1% acetic acid, 100 degrees C) hydrolyzed the ester side-chains of lipid A. It was found that the ester-linked palmitoyl group was the most labile to acid hydrolysis. Hydrolysis of the palmitoyl moiety conformed to pseudo-first-order chemical reaction kinetics with a rate constant for decomposition of heptacyl-lipid A from Enterobacter agglomerans of approximately 3.3 x 10(-3) min-1. An order of lability of lipid A acyl side-chains to acid hydrolysis was also deduced: R4' (palmitoyl) > R1' (myristoyl or hydroxymyristoyl) > R3 (hydroxymyristoyl at position 3) > R1 (oxymyristoyl group at position 3') > R2' (lauroyl). Base treatment (0.05 M NaOH in 95% EtOH, 65 degrees C) was shown to be more effective at cleaving ester-linked side-chains. In addition, mass spectral evidence suggests that opening of the pyranose rings of the disaccharide backbone of lipid A and/or removal of the phosphoryl groups may be occurring during base treatment. This study sheds light on mechanistic aspects of treatment procedures leading to the detoxification of endotoxins.

  10. Atomic resolution structure of EhpR: phenazine resistance in Enterobacter agglomerans Eh1087 follows principles of bleomycin/mitomycin C resistance in other bacteria

    Directory of Open Access Journals (Sweden)

    Itzen Aymelt

    2011-08-01

    Full Text Available Abstract Background The phenazines are redox-active secondary metabolites that a large number of bacterial strains produce and excrete into the environment. They possess antibiotic activity owing to the fact that they can reduce molecular oxygen to toxic reactive oxygen species. In order to take advantage of this activity, phenazine producers need to protect themselves against phenazine toxicity. Whereas it is believed that phenazine-producing pseudomonads possess highly active superoxide dismutases and catalases, it has recently been found that the plant-colonizing bacterium Enterobacter agglomerans expresses a small gene ehpR to render itself resistant towards D-alanyl-griseoluteic acid, the phenazine antibiotic produced by this strain. Results To understand the resistance mechanism installed by EhpR we have determined its crystal structure in the apo form at 2.15 Å resolution and in complex with griseoluteic acid at 1.01 Å, respectively. While EhpR shares a common fold with glyoxalase-I/bleomycin resistance proteins, the ligand binding site does not contain residues that some related proteins employ to chemically alter their substrates. Binding of the antibiotic is mediated by π-stacking interactions of the aromatic moiety with the side chains of aromatic amino acids and by a few polar interactions. The dissociation constant KD between EhpR and griseoluteic acid was quantified as 244 ± 45 μM by microscale thermophoresis measurements. Conclusions The data accumulated here suggest that EhpR confers resistance by binding D-alanyl-griseoluteic acid and acting as a chaperone involved in exporting the antibiotic rather than by altering it chemically. It is tempting to speculate that EhpR acts in concert with EhpJ, a transport protein of the major facilitator superfamily that is also encoded in the phenazine biosynthesis operon of E. agglomerans. The low affinity of EhpR for griseoluteic acid may be required for its physiological function.

  11. Characterization of nuclear localization signals in the type III effectors HsvG and HsvB of the gall-forming bacterium Pantoea agglomerans.

    Science.gov (United States)

    Weinthal, Dan M; Barash, Isaac; Tzfira, Tzvi; Gaba, Victor; Teper, Doron; Sessa, Guido; Manulis-Sasson, Shulamit

    2011-05-01

    HsvG and HsvB, two paralogous type III effectors of the gall-forming bacteria Pantoea agglomerans pv. gypsophilae and P. agglomerans pv. betae, determine host specificity on gypsophila and beet, respectively. They were previously shown to be DNA-binding proteins imported into host and non-host nuclei and might act as transcriptional activators. Sequence analysis of these effectors did not detect canonical nuclear localization signals (NLSs), but two basic amino acid clusters designated putative NLS1 and NLS2 were detected in their N-terminal and C-terminal regions, respectively. pNIA assay for nuclear import in yeast and bombardment of melon leaves with each of the NLSs fused to a 2xYFP reporter indicated that putative NLS1 and NLS2 were functional in transport of HsvG into the nucleus. A yeast two-hybrid assay showed that HsvB, HsvG, putative NLS1, putative NLS2, HsvG converted into HsvB, or HsvB converted into HsvG by exchanging the repeat domain, all interacted with AtKAP-α and importin-α3 of Arabidopsis thaliana. Deletion analysis of the NLS domains in HsvG suggested that putative NLS1 or NLS2 were required for pathogenicity on gypsophila cuttings and presumably for import of HsvG into the nucleus. This study demonstrates the presence of two functional NLSs in the type III effectors HsvG and HsvB.

  12. Self-transmissible nif plasmid (pEA9) of Enterobacter agglomerans 339: molecular cloning and evidence for the existence of similar nif clusters on dissimilar plasmids in Enterobacter strains.

    Science.gov (United States)

    Steibl, H D; Siddavattam, D; Klingmüller, W

    1995-11-01

    A cosmid library was generated to the 200-kb self-transmissible nif plasmid pEA9 isolated from Enterobacter agglomerans 339. The cosmid clone identified to contain the complete nif cluster was used to determine the nif gene organization and the physical map. The restriction pattern and nif gene organization of this nif cluster showed remarkable similarities to the nif cluster identified on the 110-kb plasmid pEA3 of Enterobacter agglomerans 333. Nucleotide sequence of several randomly selected regions of the nif cluster of pEA9 showed 96% similarity when compared to the known sequences of the nif cluster of pEA3. However, the homology ended abruptly at the flanking regions of the nif clusters and no similarity could be detected with the rest of the DNA of these plasmids. This reveals the existence of similar nif clusters on dissimilar plasmids, implying the horizontal transfer of the entire nif gene cluster.

  13. Benefícios do treinamento de força em crianças e adolescentes em idade escolar : uma revisão de literatura

    OpenAIRE

    Guimarães, Juliano da Silva

    2012-01-01

    O objetivo desta revisão literária foi de observar os benefícios que o treinamento de força pode trazer a crianças e adolescentes. Levando em conta as diversas variáveis do treinamento, bem como o nível maturacional de tais crianças e adolescentes. Comparando os aspectos fisiológicos do exercício em crianças e adolescentes com os ocorridos em adultos, e como é sugerido o treinamento de força para tal faixa etária.

  14. Enzimas proteolíticas do látex de diversas variedades de Ficus Carica L. Proteolytic enzimes from several varieties of Ficus Carica L.

    Directory of Open Access Journals (Sweden)

    Valdemiro C. Sgarbieri

    1965-01-01

    Full Text Available O presente trabalho consistiu na separação das enzimas proteolíticas do látex de dez variedades diferentes de Ficus carica L. Tôdas as variedades estudadas são cultivadas na Califórnia (E.U.A.. O método empregado foi o de carboximetil celulose (CM-celulose. O objetivo principal do trabalho foi verificar se havia diferenças entre os látices das diversas variedades de figo, no que diz respeito a número, quantidade e propriedades de suas enzimas proteolíticas. Difrenças quantitativas e qualitativas foram encontradas. As variedades Kadota e Calimyrna apresentaram-se, respectivamente, com 10 e 4 componentes proteolíticos ativos. A porcentagem da atividade recuperada foi calculada para cada variedade, bem como a contribuição porcentual de cada componente de uma mesma variedade, com relação à proteína total recuperada da coluna. A atividade específica foi calculada, e apresentou variação para o mesmo componente, nas diversas variedades.Latex from 10 varieties of Ficus carica were fractionated by column-chro-matograpliy on CM-cellulose under the same conditions. Both qualitative and quantitative differences were found among different varieties. The percentage of activity recovered was calculated for all varieties as well as the percentage of protein and activity that the different components contributed to the total protein and activity recovered from the column. The percentage of the total activity recovered from the column ranged from 62.6% in Adriatic to 99% for the Beall variety. The most complex latex in terms of proteolytic activity was from Kadola which had 10 active components. In this variety component 2 accounted for 2.80% of the total ativity while component 10 accounted for 29.6%. The specific activity of the components in this variety ranged from 1.28% for component 1 to 7.52 for component 10. The least complex latices were obtained from Calimyrna and Blanquette which had 4 components each. In the Calimyrna latex

  15. A TERCEIRIZAÇÃO NA SAÚDE PÚBLICA: FORMAS DIVERSAS DE PRECARIZAÇÃO DO TRABALHO

    Directory of Open Access Journals (Sweden)

    Graça Druck

    Full Text Available Resumo Apresentam-se, neste ensaio, algumas reflexões sobre a terceirização no serviço público a partir da ótica da sociologia do trabalho. Toma-se como referência a implantação das organizações sociais, que se tornou forte realidade na saúde pública nos últimos 15 anos, e que, a partir de 2015, por decisão do Supremo Tribunal Federal, foi validada como constitucional, com o que se negou o pedido de ação direta de inconstitucionalidade. Dessa forma, liberou-se a terceirização para todos os serviços essenciais sob responsabilidade do Estado. Também é objeto de discussão a implantação da Empresa Brasileira de Serviços Hospitalares, que, embora mais recente que as organizações sociais, já apresenta resultados tão nocivos quanto elas. A tese defendida é que a terceirização do serviço público no Brasil é um dos mecanismos mais eficientes de desmonte do conteúdo social do Estado e de sua privatização, que ocorre mediante formas diversas de precarização do trabalho, pois a terceirização — por meio de organizações sociais, organizações da sociedade civil de interesse público, empresas privadas, empresas públicas de direito privado, parcerias, dentre outras — é o meio principal que as forças políticas neoliberais encontraram para atacar o coração de um Estado social e democrático: os trabalhadores que constituem o funcionalismo público.

  16. Localization and physical mapping of a plasmid-borne 23-kb nif gene cluster from Enterobacter agglomerans showing homology to the entire nif gene cluster of Klebsiella pneumoniae M5a1.

    Science.gov (United States)

    Singh, M; Kreutzer, R; Acker, G; Klingmüller, W

    1988-01-01

    A physical and genetical map of the plasmid pEA3 indigenous to Enterobacter agglomerans is presented. pEA3 is a 111-kb large plasmid containing a 23-kb large cluster of nif genes which shows extensive homology (Southern hybridization and heteroduplex analysis) to the entire nif gene cluster of Klebsiella pneumoniae (Kp) M5a1. All the nif genes on pEA3 are organized in the same manner as in K. pneumoniae, except nifJ, which is located on the left end of pEA3 nif gene cluster (near nifQB). A BamHI restriction map of pEA3 and a detailed restriction map of the 23-kb nif region on pEA3 is also presented. The nif genes of pEA3 showed a low level of acetylene reduction in Escherichia coli, demonstrating that these genes are functional and contain the whole genetic information required to fix nitrogen. The origin of vegetative replication (OriV) of pEA3 was localized about 5.5 kb from the right end of the nif gene cluster. In addition to pEA3, large plasmids from four other strains of E. agglomerans showed homology to all the Kp nif genes tested, indicating that in diazotrophic strains of E. agglomerans nif genes are usually located on plasmids. In contrast, in most of the free-living, nitrogen-fixing bacteria the nif genes are on chromosome.

  17. Efeitos do treinamento concorrente na força e ativação muscular, capacidade aeróbica e em hormônios e esteróides em homens idosos

    OpenAIRE

    Eduardo Lusa Cadore

    2009-01-01

    O treinamento concorrente de força e endurance têm sido amplamente investigado em diversas populações. Contudo, poucos estudos compararam seus efeitos com o treino de força e endurance isolados em indivíduos idosos. Sendo assim, o objetivo desse estudo foi investigar os efeitos do treinamento concorrente na força e ativação muscular, capacidade de endurance e concentrações hormonais em homens idosos. Vinte e três homens saudáveis (65 ± 4 anos) foram divididos em 3 grupos: treino concorrente (...

  18. Identificação de fenótipos de electroencefalograma para neuroreabilitação em idosos

    OpenAIRE

    Ferreira, Daniela Patrícia da Silva

    2011-01-01

    Dissertação de mestrado integrado em Engenharia Biomédica (área de especialização em Electrónica Médica) Com a progressão da idade, o corpo humano e o cérebro em especial sofrem diversas alterações que os tornam mais frágeis. O envelhecimento cerebral leva a uma perda progressiva da plasticidade cerebral, o que leva a dificuldades em determinados processos cognitivos, tais como a memória, a concentração, a flexibilidade cognitiva e a tomada de decisão, entre outros. O envelheci...

  19. Efeito da quercetina nas atividades fosfatasicas e seu efeito protetor na hepatotoxicidade induzida pelo acetaminofeno em camundongos

    OpenAIRE

    Camila de Andrade Camargo

    2007-01-01

    Resumo: Os flavonóides são fitocompostos polifenólicos, caracterizados quimicamente como heterosídeos flavônicos. Apresentam diversas atividades biológicas devido às suas propriedades antioxidantes e habilidades em modular a atividade de diversas enzimas ou receptores celulares, tornando-os responsáveis pelo efeito protetor contra doenças relacionadas ao sistema cardiovascular; certas formas de câncer e doenças de fotossensibilidade e envelhecimento. As fosfatases ácidas, enzimas que hidrolis...

  20. Estudo da resposta imunitária à vacinação anti-meningocócica com polissacarídeos de diversas procedências

    Directory of Open Access Journals (Sweden)

    Eduardo de Azeredo Costa

    1977-04-01

    Full Text Available Um estudo duplamente cego sobre a capacidade de indução de anticorpos séricos anti-meningocócicos por polissacarfdeos capsulares A e C, detectados pela técnica de hemaglutinação passiva, foi realizado no período de outubro a dezembro de 1974. Analisaram-se as respostas imunogênicas de polissacarídeos isolados e combinados de três procedências, entre as quais a de um laboratório que se propunha a produzi-los em larga escala e que já os havia testado em um grupo de voluntários sem reações colaterais importantes. Duzentos e trinta e oito voluntários, funcionários e internos de uma penitenciária, foram divididos em 6 grupos aos quais foram administrados toxóide tetânico e cinco diferentes tipos de vacina. Colheitas de sangue imediatamente antes e quatro semanas após a inoculação foram realizadas e a conversão soro lógica analisada. As vacinas polissacarídeas C mostraram boa resposta imunogênica, enquanto a vacina A mostrou resposta mais pobre. As vacinas em experimentação que continham polissacarídeo A não demonstraram poder antigênico, apresentando, inclusive, resposta paradoxal, com a formação de anticorpos anti-meningocócicos C. Reações colaterais nos grupos submetidos a estas vacinas, embora de pequena intensidade, mostraram-se mais freqüentes do. que nos outros grupos, sugerindo, como depois se evidenciariam, problemas de purificação. A inoculação de vacinas mono vai entes determinou uma queda de níveis de anticorpos não específicos (queda de anti-polissacarfdeo A quando a vacina era polissacarfdea C e vice-versa, sugerindo, juntamente com achados de outros estudos, um possível mecanismo de depressão imunitária que merece ser melhor investigado.

  1. Análise do movimento em rituais umbandistas

    Directory of Open Access Journals (Sweden)

    Marielle Kellermann Barbosa

    Full Text Available A presente pesquisa teve como objetivo fazer a análise do movimento em rituais umbandistas e, dessa forma, contribuir para o conhecimento da sua linguagem corporal, assim como fornecer subsídios para o desenvolvimento de uma etnopsicologia brasileira. Para efeito de análise, foi utilizado o método Laban, que visa analisar, segundo elementos de esforço, a movimentação corporal, assim como a tonicidade muscular, em relação ao peso, tempo, espaço e fluência. Foram examinados registros em vídeo de diversas classes de "espíritos" do panteão umbandista. A análise dos movimentos peculiares às diversas classes de "espíritos" mostrou que elas diferenciam-se umas das outras por padrões que as caracterizam em relação aos elementos de esforço analisados. Concluiu-se ser possível utilizar o método Laban para discriminar categorias do panteão umbandista e, dessa forma, acrescer o conhecimento a respeito da linguagem corporal nesse culto.

  2. Unidade de Estudos de Mastites em Pequenos Ruminantes - estudos em ovinos de regime extensivo

    OpenAIRE

    Queiroga, Cristina; Potes, Maria Eduarda; Duarte, Elsa Maria; Marinho, Artur; Bettencourt, Carlos Manuel; Matos, Claudino; Belo, Carlos Carmona; Ribeiro, José Manuel; Vilela, Cristina Lobo

    2007-01-01

    A “Unidade de Estudo de Mastites em Pequenos Ruminantes”, formalmente constituída no âmbito do Programa Nacional de Re-equipamento Científico, da Fundação para a Ciência e a Tecnologia, vem dar corpo ao trabalho de colaboração de diversas equipas de investigação que se têm debruçado sobre a problemática das infecções intramamárias em pequenos ruminantes, nos seus aspectos de saúde animal, saúde pública veterinária e qualidade e segurança alimentar. Tem como principal objectivo criar condições...

  3. Epidemiology and molecular characterization of extended-spectrum beta-lactamase-producing Enterobacter spp., Pantoea agglomerans, and Serratia marcescens isolates from a Bulgarian hospital.

    Science.gov (United States)

    Markovska, Rumyana Donkova; Stoeva, Temenuga Jekova; Bojkova, Kalina Dineva; Mitov, Ivan Gergov

    2014-04-01

    Forty-two extended-spectrum beta-lactamase (ESBL)-producing isolates of Enterobacter aerogenes, Enterobacter cloacae, Pantoea agglomerans, and Serratia marcescens, collected consecutively during the period January-November 2011 from the University Hospital in Varna, Bulgaria, were studied to characterize their ESBLs by isoelectric focusing, group-specific PCR, and sequencing. The epidemiological relationship was evaluated by random amplified polymorphic DNA analysis (RAPD). Transferability of ESBL genes was determined by conjugation experiments. Plasmid analysis was done by replicon typing and PstI fingerprinting. The overall rate of ESBL production was 20%. The most widespread enzyme was CTX-M-3, found in 64%. It was dominant in E. aerogenes (100%) and S. marcescens (83%). SHV-12, CTX-M-3, and CTX-M-15 were found among E. cloacae isolates in 50%, 35%, and 45%, respectively. Three main CTX-M-3-producing epidemic clones of E. aerogenes and S. marcescens have been detected. Among E. cloacae isolates, six different RAPD profiles were discerned. The plasmids harboring blaCTX-M-3 belonged to IncL/M type and demonstrated similar PstI fingerprinting profiles. IncFII plasmids were detected in two CTX-M-15-producing E. cloacae isolates. Our results demonstrate wide intrahospital dissemination of clonal E. aerogenes and S. marcescens isolates, carrying IncL/M conjugative plasmids.

  4. A Lipopolysaccharide from Pantoea Agglomerans Is a Promising Adjuvant for Sublingual Vaccines to Induce Systemic and Mucosal Immune Responses in Mice via TLR4 Pathway

    Science.gov (United States)

    Kiyotoh, Eiji; Okazaki, Arimichi; Gomi, Yasuyuki; Tanimoto, Takeshi; Takeuchi, Osamu; Akira, Shizuo; Hori, Mitsuhiko

    2015-01-01

    A lipopolysaccharide from Pantoea agglomerans (LPSpa) has been applied to various fields for human use as a Toll-like receptor 4 ligand and its safety has been confirmed. Here, we showed for the first time the application of LPSpa as an effective mucosal adjuvant for activating vaccine-induced antigen specific immune responses. Mice sublingually immunized with influenza vaccine (HA split vaccine) with LPSpa induced both HA-specific IgG (systemic) and IgA (mucosal) antibody responses, which led to a significant increase in survival rate against lethal influenza virus challenge compared with subcutaneous vaccination. After sublingual administration of ovalbumin with LPSpa, ovalbumin-specific mucosal IgA responses were induced at both mucosal surfaces close to the immunized site and at remote mucosal surfaces. Sublingual administration of LPSpa evoked local antigen-uptake by dendritic cells in cervical lymph nodes. LPSpa induced cytokine production and the maturation and proliferation of innate immune cells via Toll-like receptor 4 in dendritic cells. Collectively, these results suggest that LPSpa can be used as an effective mucosal adjuvant to stimulate and activate local innate immune cells to improve and enhance mucosal vaccine potency against various pathogens. PMID:25978818

  5. Influence of diluent and sample processing methods on the recovery of the biocontrol agent Pantoea agglomerans CPA-2 from different fruit surfaces.

    Science.gov (United States)

    Torres, R; Viñas, I; Usall, J; Remón, D; Teixidó, N

    2012-08-01

    Determining the populations of biocontrol agents applied as a postharvest treatment on fruit surfaces is fundamental to the assessment of the microorganisms' ability to colonise and persist on fruit. To obtain maximum recovery, we must develop a methodology that involves both diluent and processing methods and that does not affect the viability of the microorganisms. The effect of diluent composition was evaluated using three diluents: phosphate buffer, peptone saline and buffered peptone saline. An additional study was performed to compare three processing methods (shaking plus sonication, stomaching and shaking plus centrifugation) on the recovery efficiency of Pantoea agglomerans strain CPA-2 from apples, oranges, nectarines and peaches treated with this biocontrol agent. Overall, slight differences occurred among diluents, although the phosphate buffer maintained the most ideal pH for CPA-2 growth (between 5.2 and 6.2). Stomaching, using the phosphate buffer as diluent, was the best procedure for recovering and enumerating the biocontrol agent; this fact suggested that no lethal effects from naturally occurring antimicrobial compounds present on the fruit skins and/or produced when the tissues were disrupted affected the recovery of the CPA-2 cells, regardless of fruit type. The growth pattern of CPA-2 on fruits maintained at 20°C and under cold conditions was similar to that obtained in previous studies, which confirms the excellent adaptation of this strain to conditions commonly used for fruit storage. Copyright © 2012 Elsevier B.V. All rights reserved.

  6. Use of Gentamicine sulfate for the control of Pantoea agglomerans, contaminant of the Solanum tuberosum L cv. Desirée in vitro multiplication

    Directory of Open Access Journals (Sweden)

    Yelenys Alvarado-Capó

    2006-10-01

    Full Text Available Bacterial contamination is one of the principal problems of plant tissue culture. The use of natural or synthetic antimicrobial substances represent an alternative for it solution. In potato micropropagation bacterial contamination produce serious damages. In this paper the effect of Gentamicine sulfate on control of high frequently contaminant was evaluated. The microorganism was isolated from media culture of potato cv. Desirée in vitro plants in multiplication stage. Traditional tintorial, bioquemical and physiological test were performed for bacteria identification together with BIOLOG bacteria identification system. Besides, the minimal inhibitory concentration (MIC and minimal bactericidal concentration (MBC of Gentamicine sulfate were determined and it antimicrobial and phytotoxic effect in multiplication stage were evaluated too. Pantoea agglomerans was identificated as potato contaminant and show sensibility to Gentamicine sulfate. The MIC was 0.625 mg.l-1 and the MBC was 1.25 mg.l-1. The antibiotics controlled the contamination in the 80% of contaminated explants without phytotoxicity at 2.5 mg.l-1. Key words: bacteria, in vitro culture, microbial contamination, potato

  7. A Lipopolysaccharide from Pantoea Agglomerans Is a Promising Adjuvant for Sublingual Vaccines to Induce Systemic and Mucosal Immune Responses in Mice via TLR4 Pathway.

    Science.gov (United States)

    Fukasaka, Masahiro; Asari, Daisuke; Kiyotoh, Eiji; Okazaki, Arimichi; Gomi, Yasuyuki; Tanimoto, Takeshi; Takeuchi, Osamu; Akira, Shizuo; Hori, Mitsuhiko

    2015-01-01

    A lipopolysaccharide from Pantoea agglomerans (LPSpa) has been applied to various fields for human use as a Toll-like receptor 4 ligand and its safety has been confirmed. Here, we showed for the first time the application of LPSpa as an effective mucosal adjuvant for activating vaccine-induced antigen specific immune responses. Mice sublingually immunized with influenza vaccine (HA split vaccine) with LPSpa induced both HA-specific IgG (systemic) and IgA (mucosal) antibody responses, which led to a significant increase in survival rate against lethal influenza virus challenge compared with subcutaneous vaccination. After sublingual administration of ovalbumin with LPSpa, ovalbumin-specific mucosal IgA responses were induced at both mucosal surfaces close to the immunized site and at remote mucosal surfaces. Sublingual administration of LPSpa evoked local antigen-uptake by dendritic cells in cervical lymph nodes. LPSpa induced cytokine production and the maturation and proliferation of innate immune cells via Toll-like receptor 4 in dendritic cells. Collectively, these results suggest that LPSpa can be used as an effective mucosal adjuvant to stimulate and activate local innate immune cells to improve and enhance mucosal vaccine potency against various pathogens.

  8. A Lipopolysaccharide from Pantoea Agglomerans Is a Promising Adjuvant for Sublingual Vaccines to Induce Systemic and Mucosal Immune Responses in Mice via TLR4 Pathway.

    Directory of Open Access Journals (Sweden)

    Masahiro Fukasaka

    Full Text Available A lipopolysaccharide from Pantoea agglomerans (LPSpa has been applied to various fields for human use as a Toll-like receptor 4 ligand and its safety has been confirmed. Here, we showed for the first time the application of LPSpa as an effective mucosal adjuvant for activating vaccine-induced antigen specific immune responses. Mice sublingually immunized with influenza vaccine (HA split vaccine with LPSpa induced both HA-specific IgG (systemic and IgA (mucosal antibody responses, which led to a significant increase in survival rate against lethal influenza virus challenge compared with subcutaneous vaccination. After sublingual administration of ovalbumin with LPSpa, ovalbumin-specific mucosal IgA responses were induced at both mucosal surfaces close to the immunized site and at remote mucosal surfaces. Sublingual administration of LPSpa evoked local antigen-uptake by dendritic cells in cervical lymph nodes. LPSpa induced cytokine production and the maturation and proliferation of innate immune cells via Toll-like receptor 4 in dendritic cells. Collectively, these results suggest that LPSpa can be used as an effective mucosal adjuvant to stimulate and activate local innate immune cells to improve and enhance mucosal vaccine potency against various pathogens.

  9. Seroprevalence of Kaposi's sarcoma-associated herpesvirus in various populations in Cuba Seroprevalencia del herpesvirus asociado con el sarcoma de Kaposi en diversas poblaciones en Cuba

    Directory of Open Access Journals (Sweden)

    Vivian Kourí

    2004-05-01

    , Jamaica, and the United States of America, our results suggest that KSHV infection is uncommon in some populations in the Western Hemisphere and that KSHV is largely confined to patients with AIDS-associated KS.OBJETIVO: Se sabe poco acerca de la prevalencia y distribución en el Caribe de la infección por el herpesvirus asociado con el sarcoma de Kaposi (HVSK. El objetivo del presente estudio fue determinar las tasas de seropositividad a HVSK en diversas poblaciones en Cuba. MÉTODOS: Durante el período de 1998 a 2002 se examinaron muestras de suero de 410 personas en Cuba. El tamizaje serológico de los anticuerpos contra HVSK se realizó en dos pasos con las siguientes técnicas: 1 ensayo de inmunofluorescencia indirecta (IFI con reactividad específica a la porción del antígeno de HVSK asociado a la latencia (ANAL que está codificada por la plantilla de lectura abierta 73 (ORF73, y 2 inmunoblot (Western confirmatorio, usando como antígeno blanco la proteína ORF65.2 de HVSK recombinante, que tiene 20 kilodaltones y se expresa durante la infección lítica. Cinco poblaciones diferentes fueron estudiadas: 1 45 pacientes de sida con sarcoma de Kaposi (sida-KS; 2 154 pacientes infectados por VIH-1 pero sin signos clínicos de sarcoma de Kaposi (SK; 3 171 donantes de sangre con seronegatividad a VIH; 4 27 receptores consecutivos de transplantes renales con seronegatividad a VIH y 5 13 contactos (sexuales o por parentesco de los pacientes de sida con SK. RESULTADOS: De las 45 personas con sida y SK, 35 (77,8% mostraron seropositividad a HVSK. Treinta y dos (20,8% de los 154 pacientes con seropositividad a VIH pero sin SK mostraron seropositividad a HVSK, y 6 de los 13 contactos (46,2% de pacientes con SK estaban infectados por HVSK. A diferencia de otros investigadores, los autores del presente estudio no encontraron ninguna asociación entre la seropositividad a HVSK y la actividad homosexual o bisexual en personas de sexo masculino en las poblaciones cubanas

  10. O que conduz a formação de cooperação tecnológica entre universidade e indústria em sistemas de inovação menos desenvolvidos? Evidências do Brasil

    OpenAIRE

    Ana Luiza L. de Araújo Burcharth

    2011-01-01

    Diversas formas de colaboração interorganizacional têm sido reconhecidas na literatura por seu potencial em promover aprendizagem e capacidades inovadoras, em especial aquelas envolvendo interações entre universidade e empresa. No entanto, elas são relativamente escassas em Sistemas de Inovação pouco desenvolvidos, como o brasileiro, e pouco se sabe sobre o porquê ou como estão estabelecidas. Com base em um estudo de caso comparativo, este trabalho investiga o contexto em que a cooperação tec...

  11. Uso do arprinocid, nicarbazina e 3-5-dinitro-o-toluamida (d.o.t) no controle da coccidiose em reprodutoras pesadas para produção de pintos de corte

    OpenAIRE

    Fernando Cristino Barbosa

    1984-01-01

    O presente experimento foi realizado com o objetivo de avaliar a eficiência dos anticoccídicos arprinocid, nicarbazina e 3-5-dinitro-o-toluamida (D.O.T.) em diversas dosagens e combinações, em programas de controle da coccidiose em galinhas matrizes. Foram utilizadas 840 matrizes, da linhagem comercial Cobb, de 1 dia a 20 semanas de idade. Estas aves foram alojadas em um galpão convencional, dividido em vários boxes. Como material de "cama" foi utilizado o cepilho de madeira. A ração utilizad...

  12. Caracterização funcional das aquaporinas-3 e 7 de mamífero por expressão heteróloga em leveduras

    OpenAIRE

    Mósca, Andreia Filipa Bonecas

    2013-01-01

    Dissertação para obtenção do Grau de Mestre em Bioquímica Estrutural e Funcional As aquaporinas (AQPs) são proteínas intrínsecas de membrana, fisiologicamente importantes, que transportam água (aquaporinas ortodoxas) e também, em alguns casos, outros pequenos solutos como o glicerol (aquagliceroporinas). Em mamíferos, treze aquaporinas (AQP0-AQP12) são conhecidas até à data, sendo diferencialmente expressas em diversas células e tecidos. Recentemente, foram encontradas evidê...

  13. Estudo linguístico comparativo sobre onomatopeias em histórias em quadrinhos: Português / Alemão

    OpenAIRE

    Caldas,Raoni Naraoka de

    2011-01-01

    Nesta pesquisa foi investigado o uso peculiar das onomatopeias na linguagem das histórias em quadrinhos e como o processo de criação de novas expressões nesse contexto respeita o sistema fonético e fonotático de cada língua. Foi realizada uma pesquisa empírica, para a qual falantes nativos de língua portuguesa e alemã foram solicitados a sugerir ou criar as onomatopeias que julgassem adequadas para diversas cenas de histórias em quadrinhos[1] que lhes foram apresentadas editadas, com as onoma...

  14. Enteroparasitas em populações usuárias de diferentes sistemas de abastecimento de água em Viçosa-MG

    OpenAIRE

    Oliveira, Adriana Aguiar

    2004-01-01

    As doenças relacionadas com a água são importantes problemas de saúde pública no Brasil e em diversas partes do mundo. Dentre estas, destacam-se as parasitoses intestinais ou enteroparasitoses, por se associarem freqüentemente a quadros de diarréia crônica e desnutrição, acometendo, principalmente, as faixas etárias mais jovens. O presente trabalho objetivou realizar pesquisa sobre enteroparasitas no município de Viçosa em três cortes populacionais de crianças menores de sete anos de idade, b...

  15. Malformações antenais em zangões de Apis mellifera de ocelos claros (Hymenoptera, Apidae)

    OpenAIRE

    CHAUD-NETTO, J.

    2000-01-01

    Antenas com malformações diversas, extraídas de zangões de Apis mellifera de ocelos claros, foram desenhadas e dissecadas para a contagem de discos olfativos dos segmentos morfologicamente alterados, em comparação com as antenas normais de seus irmãos. Alguns zangões apresentaram anormalidades morfológicas em um dos segmentos da antena direita ou esquerda, mas outros possuíam dois ou mais segmentos alterados na mesma antena. Zangões com malformações em ambas as antenas também foram observados...

  16. MNLAC: uma proposta de modelagem de fluxo de navegação baseada em lógica modal

    OpenAIRE

    Oliveira, Daniele Carvalho

    2011-01-01

    Atualmente, diversas técnicas estão sendo exploradas com objetivo de melhorar o processo de projeto e desenvolvimento de interfaces com usuário. Novas técnicas criadas de software procuram suprir a deficiência na área. A complexidade dos requisitos exigidos pelo usuário e a dificuldade em entende-los são as principais causas dos problemas enfrentados ao projetar a interface de um sistema. Para projetar um bom sistema, não basta apenas focar em suas funcionalidades e em como elas serão impleme...

  17. Identificação de genes associados a produção de ocratoxina A em Aspergillus westerdijkiae

    OpenAIRE

    Daniele Sartori

    2009-01-01

    Micotoxinas, metabólitos secundários de fungos, são compostos tóxicos encontrados em uma grande variedade de alimentos que, quando ingeridos, podem colocar em risco a saúde humana e animal. Dentre as diversas micotoxinas, especial interesse tem sido dado à ocratoxina A (OA), produzida por algumas espécies de Aspergillus e Penicillium. Esta micotoxina é encontrada com frequência principalmente em alimentos como grãos e uvas e, quando ingerida e acumulada, pode causar efeitos como, nefrotoxicid...

  18. Fermentação etanólica em mostos de hidrolisados de amido de mandioca

    OpenAIRE

    Urbano, Luiz Henrique [UNESP

    2012-01-01

    O etanol no Brasil representa uma fonte alternativa de energia fortemente empregada como bicombustível, em mistura à gasolina como etanol anidro ou diretamente como álcool carburante hidratado. Há diversas matérias primas capazes de fornecer carboidratos para ser convertidas em etanol por processo fermentativo, sendo a cana-de-açúcar a mais utilizada em escala industrial. Existem outras fontes que podem ser empregadas de forma alternativa, considerando fatores como produtividade, condições ed...

  19. Avaliação da atividade antiinflamatória do coentro (Coriandrum sativum L.) em roedores

    OpenAIRE

    Zanusso-Junior,G; Melo,J.O; Romero,A.L; Dantas,J.A; Caparroz-Assef,S.M; Bersani-Amado,C.A; Cuman,R.K.N

    2011-01-01

    Coriandrum sativum L. (Umbelliferae), conhecido popularmente por coentro, é uma planta doméstica cultivada nas diversas partes do mundo, inclusive no Brasil. As folhas e frutos do coentro são utilizados como condimento em culinária e na medicina popular como analgésica, antirreumática, carminativa e colagoga. O objetivo deste estudo foi avaliar o efeito do tratamento com o óleo essencial (OEC) e o extrato hidroalcóolico (EHC) do coentro em modelos experimentais de inflamação em roedores. A at...

  20. A toxidez de diversas lantanas para bovinos e ovinos no Brasil The toxicity of diverse lantanas for cattle and sheep in Brazil

    Directory of Open Access Journals (Sweden)

    Marilene de Farias Brito

    2004-09-01

    Full Text Available Neste estudo sobre a toxidez das lantanas para bovinos e ovinos, verificou-se situação semelhante à que ocorre na Austrália, que nem todas as espécies de Lantana e nem todos os taxa de Lantana camara que ocorrem no Brasil, são tóxicos. Verificou-se, que foram tóxicas as amostras de lantanas procedentes de Boa Vista (RO, Castanhal (PA, Cáceres (MT, Serra Talhada (PE, Cabo Frio e Quatis (RJ e Canoinhas (SC. Outras amostras procedentes de Cruzeiro do Sul (Acre, Castanhal (PA, Chapada dos Guimarães e Lambari d´Oeste (MT, Jaguaribe (CE, Vitória da Conquista e Wanderley (BA, Vitória (ES, Vassouras, Parati e Itaguaí (RJ não revelaram toxidez nas doses administradas (40 g/kg. A dose letal das amostras submetidas à experimentação no Brasil foi bastante constante (40 g/kg, com duas exceções: as folhas frescas da lantana procedente de Canoinha (SC foram muito mais tóxicas (10 g/kg e as folhas frescas e as dessecadas da lantana de Serra Talhada (PE foram muito menos tóxicas, pois somente causaram intoxicação não-letal com a dose de 40g/kg/dia administradas durante 30 dias. Históricos sobre a observação de fotossensibilização em bovinos, ligados à ingestão de lantanas foram obtidos em Serra Talhada (PE, Cáceres (MT, Cabo Frio (RJ, Quatis (RJ e Canoinhas (SC. Verificou-se que não é possível estabelecer uma correlação entre a cor das inflorescências das lantanas e sua toxidez, confirmando a constatação feita na Austrália, que o potencial de intoxicar não está necessariamente relacionada com a cor das flores.Experiments on the toxicity of Lantana spp for cattle and sheep revealed a similar situation as is reported from Australia. Neither all Lantana species nor all varieties which occur in Brazil are poisonous. Lantana samples collected at Boa Vista (Roraima, Castanhal (Pará, Cáceres (Mato Grosso, Serra Talhada (Pernambuco, Cabo Frio and Quatis (Rio de Janeiro, and Canoinhas (Santa Catarina were toxic. All the other

  1. Independência ou morte em Salvador: o cotidiano da capital da Bahia no contexto do processo de independência brasileiro (1821-1823)

    OpenAIRE

    Silva, Marcelo Renato Siquara

    2012-01-01

    175f. Esta pesquisa pretende analisar as diversas facetas vinculadas ao processo de independência brasileiro na Bahia (1821-1823). As questões que envolvem a política, a cultura, a sociedade e a economia baiana são objeto de inúmeras discussões. Contudo, chamamos a atenção para o seguinte fato: o nosso foco principal de discussão encontrase centrado nas diversas alterações provocadas pela guerra civil junto ao cotidiano da Cidade do Salvador. Em função disto, os confrontos e...

  2. Mineração de dados em sistemas OLAP

    OpenAIRE

    Sequeira, Ana Isabel Gonçalves

    2013-01-01

    Dissertação de mestrado em Engenharia Informática As diversas vantagens que os data warehouses têm proporcionado no que toca ao armazenamento e processamento de informação levaram a uma subida substancial na aquisição deste tipo de estruturas por parte das organizações. De facto, os data warehouses são caracterizados por um modelo de dados que permite, entre várias opções, realizar pesquisas complexas, selecionar conjuntos de dados de maior interesse, executar operações de sintetização, fa...

  3. SISTEMA PARA REDUZIR O CONSUMO EM STAND-BY - ECOENERGY

    Directory of Open Access Journals (Sweden)

    Jorge Sales

    2014-12-01

    Full Text Available Este artigo trata-se da elaboração de uma central para economia de energia, automação e controle residencial, basicamente eliminando o consumo de energia no modo stand by. Esse sistema a ser detalhado possui leds nos equipamentos eletrônicos, foi elaborado em base da tecnologia Zigbee, especificamente, para aplicações de economia de energia com baixo custo de aquisição e fácil instalação, tornando-o acessível a usuários de diversas classes face a crescente necessidade do mercado.

  4. Dimensões de qualidade em ambientes virtuais de aprendizagem

    OpenAIRE

    Silvio Carvalho Neto

    2009-01-01

    O desenvolvimento comercial da internet possibilitou o surgimento de sistemas de informação que se apoiam no uso da world wide web como plataforma. Esses sistemas baseados na web (web-based systems) têm aplicações em diversas áreas e setores econômicos. No ramo educacional, eles têm sido aplicados como base para os processos de ensino e aprendizagem via computador (e-learning) e são, comumente, denominados de Ambientes Virtuais de Aprendizagem (AVA). Os AVAs podem ser utilizados tanto para o ...

  5. Avaliação de 166 ETES em operação no país, compreendendo diversas tecnologias. Parte 2: influência de fatores de projeto e operação

    OpenAIRE

    Oliveira,Sílvia M. A. Corrêa; von Sperling,Marcos

    2005-01-01

    O estudo avalia a influência de fatores de projeto e de operação no desempenho de estações de tratamento de esgotos, considerando a concentração efluente e eficiência de remoção de DBO. Apenas quatro das seis tecnologias de tratamento estudadas na Parte 1 forneceram dados suficientes para análise: lagoas facultativas, lagoas anaeróbias seguidas por lagoas facultativas, lodos ativados e reatores UASB operando isoladamente. O objetivo da pesquisa foi verificar a influência das condições de carg...

  6. Degradation of the insecticide ethyl parathion in different environmental matrices by gamma radiation from Cobalt-60; Degradacao do inseticida paration etilico em diversas matrizes ambientais por meio de radiacao ionizante gama do cobalto-60

    Energy Technology Data Exchange (ETDEWEB)

    Luchini, Luiz Carlos

    1995-07-01

    This work studies the use of gamma radiation from cobalt-60 to induce parathion degradation in different matrices, as well as, quantified GC-NPD and identified by GC-MS, the radiolysis resulting products. Results show that the insecticide was completely degraded in aqueous solution after treatment with 1.0 kGy dosis in a dosis rate of 3.12 kGy h{sup -1}. In methanol, parathion was completely degraded only with 30 kGy at 3.12 kGy h{sup -1}. The metabolites detected after radiolysis were the same formed by biological degradation, i.e, p-nitrophenol, p-aminophenol, paraoxon and aminoparathion. The gamma radiation also degraded paraoxon which is the most toxic metabolite of parathion. It was verified that, not only the total radiation, but also the dosis rate supplied to the aqueous solution had a significant effect on the insecticide degradation, and the formation of metabolites occurred in a selective way respecting the dosis and dosis rate. Otherwise, the gamma radiation did almost not degraded the parathion adsorbed in solid matrices as rice, moist and dry soil, even using dosis of 5,30 and 50 kGy, respectively. The parathion yield and the dosis of gamma radiation needed for 50% reduction of the insecticide initial concentration in aqueous solution were also calculated and presented. Thus, it was verified that irradiation of parathion besides to be an important instrument for environmental decontamination of this pesticide in aqueous matrix, it allows the production of parathion metabolites for ecotoxicological studies. (author)

  7. Óleos essenciais de folhas de diversas espécies: propriedades antioxidantes e antibacterianas no crescimento espécies patogênicas

    Directory of Open Access Journals (Sweden)

    Cíntia Alvarenga Santos Fraga Miranda

    Full Text Available RESUMO Os óleos essenciais apresentam possibilidade de serem empregados nas indústrias de alimentos, bebidas, produtos de higiene pessoal e cosméticos para evitar ou reduzir a deterioração lipídica e a contaminação por micro-organismos. Este trabalho teve como objetivos avaliar as propriedades funcionais antimicrobianas e antioxidantes de óleos essenciais de folhas frescas de Coniza bonariensis, Parthenium hysterophorus, Tithonia diversifolia, Ambrosia polystachya, Hedychium coronarium e Baccharis dracunculifolia, extraídos por hidrodestilação. O potencial antioxidante foi avaliado pelas metodologias do consumo do radical DPPH e da inibição da oxidação do sistema β-caroteno/ácido linoleico. A sensibilidade das bactérias Salmonella Cholerasuis, Listeria monocytogenes, Staphylococcus aureus e Escherichia coli frente aos óleos essenciais foi determinada pela utilização do método de difusão em cavidade ágar. Os óleos essenciais destacaram-se pelo elevado conteúdo de terpenoides. Todos os óleos essenciais avaliados pela metodologia do sequestro do radical DPPH não apresentaram CI50 significativos. Pela metodologia do β-caroteno/ácido linoleico, os óleos essenciais de T. diversifolia e H. coronarium não apresentaram atividades significativas e os de C. bonariensis, P. hysterophorus, A. polystachya, e B. dracunculifolia apresentaram CI50 superiores a maior concentração avaliada. Os óleos essenciais das espécies C. bonariensis, T. diversifolia, H. coronarium e de B. dracunculifolia apresentaram atividade antibacteriana para bactérias Gramnegativas e Gram-positivas, com exceção do óleo volátil de P. hysterophorus, que não impediu o crescimento de nenhuma das cepas bacterianas testadas. O óleo essencial de A. polystachya apresentou potencial antibacteriano apenas nas cepas de S. aureus.

  8. Empreendedorismo social e economia criativa: uma aplicação em Projetos de orquestras infantojuvenis

    OpenAIRE

    Tatiane Lopes Duarte

    2015-01-01

    A sociedade em que vivemos enfrenta diversas situações contraditórias. Ao mesmo tempo em que a tecnologia e a inovação desenvolvem a cada dia novos produtos que promovem avanços expressivos na melhoria das condições de existência humana no Planeta, cresce assustadoramente o vácuo entre a sua disponibilidade e as possibilidades de acesso à maior parte dos seres humanos que nele habitam. Nesse contexto surge o movimento de inclusão social por meio da música clássica proporcionado pelos Projetos...

  9. Desafios da família brasileira em gerar e transmitir capital social familiar

    OpenAIRE

    Porreca, Wladimir

    2015-01-01

    Diante das diversas mudanças societárias e culturais, bem como, das ambivalências e fragmentações das políticas públicas que, em contexto brasileiro, geram o aumento de desigualdades e mazelas sociais, a família brasileira reage, se adapta, sobrevive e continua a ser fundamental e necessária para a vida humana, além de eixo principal da sociedade brasileira, principalmente, por produzir bens relacionais entre seus membros e para a sociedade. Este artigo, em seu conjunto, procura visualizar a ...

  10. Palimpsestos bíblicos em Sombra severa, de Raimundo Carrero

    OpenAIRE

    Costa, Eliene Medeiros da

    2012-01-01

    Os textos literários são configurados por meio de intercâmbio com diversas formulações discursivas presentes no âmbito da cultura. Por essa razão, os mesmos estão em constante diálogo uns com os outros, exemplificados através de relações interdiscursivas, que por vezes se apresentam como palimpsestos. Nesta perspectiva, o presente trabalho se apóia em contribuições teóricas e metodológicas sobre o discurso, particularmente, a Transtextualidade, de Genette, e a Interdiscursividade, de Mainguen...

  11. Alteration of flower color in Iris germanica L. 'Fire Bride' through ectopic expression of phytoene synthase gene (crtB) from Pantoea agglomerans.

    Science.gov (United States)

    Jeknić, Zoran; Jeknić, Stevan; Jevremović, Slađana; Subotić, Angelina; Chen, Tony H H

    2014-08-01

    Genetic modulation of the carotenogenesis in I. germanica 'Fire Bride' by ectopic expression of a crtB gene causes several flower parts to develop novel orange and pink colors. Flower color in tall bearded irises (Iris germanica L.) is determined by two distinct biochemical pathways; the carotenoid pathway, which imparts yellow, orange and pink hues and the anthocyanin pathway, which produces blue, violet and maroon flowers. Red-flowered I. germanica do not exist in nature and conventional breeding methods have thus far failed to produce them. With a goal of developing iris cultivars with red flowers, we transformed a pink iris I. germanica, 'Fire Bride', with a bacterial phytoene synthase gene (crtB) from Pantoea agglomerans under the control of the promoter region of a gene for capsanthin-capsorubin synthase from Lilium lancifolium (Llccs). This approach aimed to increase the flux of metabolites into the carotenoid biosynthetic pathway and lead to elevated levels of lycopene and darker pink or red flowers. Iris callus tissue ectopically expressing the crtB gene exhibited a color change from yellow to pink-orange and red, due to accumulation of lycopene. Transgenic iris plants, regenerated from the crtB-transgenic calli, showed prominent color changes in the ovaries (green to orange), flower stalk (green to orange), and anthers (white to pink), while the standards and falls showed no significant differences in color when compared to control plants. HPLC and UHPLC analysis confirmed that the color changes were primarily due to the accumulation of lycopene. In this study, we showed that ectopic expression of a crtB can be used to successfully alter the color of certain flower parts in I. germanica 'Fire Bride' and produce new flower traits.

  12. Regulation of nif gene expression in Enterobacter agglomerans: nucleotide sequence of the nifLA operon and influence of temperature and ammonium on its transcription.

    Science.gov (United States)

    Siddavattam, D; Steibl, H D; Kreutzer, R; Klingmüller, W

    1995-12-20

    The nucleotide sequence of a plasmid-borne 3.9 kb XhoI-SmaI fragment comprising the 3'-region of the nifM gene, the nifL and nifA genes and the 5'-region of nifB gene of Enterobacter agglomerans was determined. The genes were identified by their homology to the corresponding nif genes of Klebsiella pneumoniae. A typical sigma 54-dependent promoter and a consensus NtrC-binding motif were identified upstream of nifL. The predicted amino acid sequence of NifL showed close similarities to NifL of K. pneumoniae and Azotobacter vinelandii. However, no histidine residue was found to correspond to histidine-304 of A. vinelandii NifL, which had been proposed to be required for the repressor activity of NifL. The NifA sequence with a putative DNA binding motif (Q(x3) A(x3) G(x5)I) and an ATP binding site in the C-terminal and central domains, respectively, resembles that of other known NifA proteins. The function of the nifL and nifA genes was demonstrated in vivo using a binary plasmid system by their ability to activate a nifH promoter-lacZ fusion at different temperatures and concentrations of NH4+. Maximal promoter activity occurred at 25 degrees C, and it appears that the sensitivity of NifA to elevated temperatures is independent of NifL. The expression of nifL inhibited promoter activity in the presence of NifA when the initial NH4+ concentration in the medium exceeded 4 mM.

  13. Production of D-myo-inositol(1,2,4,5,6pentakisphosphate using alginate-entrapped recombinant Pantoea agglomerans glucose-1-phosphatase

    Directory of Open Access Journals (Sweden)

    Ralf Greiner

    2008-04-01

    Full Text Available The glucose-1-phosphatase encoding gene (agp of Pantoea agglomerans was sequenced and heterologously expressed in Escherichia coli. The enzyme showed very high homology to periplasmatic glucose-1-phosphatases of other members of the Enterobacteriaceae family. It was isolated from transformed Escherichia coli cells in a single step in high yields (32.3 ± 1.2 mg per litre of culture by Ni-NT agarose affinity chromatography to >95% purity as calculated from specific activity determinations. The purified glucose-1-phosphatase was entrapped in alginate beads with an entrapment efficiency of >80%. Temperature stability was enhanced as a consequence of entrapment, whereas pH dependence of enzyme activity was not affected. Maximum catalytic activity of entrapped glucose-1-phosphatase was found at 70°C, whereas the free enzyme exhibited maximal activity at 60°C. A single pH optimum at pH 4.5 was determined for the free and the entrapped enzyme. Kinetic parameters for the hydrolysis of sodium phytate were found to be affected by entrapment. They were determined to be K M = 0.84 mmol l-1 and k cat = 8 s-1 at pH 4.5 and 37°C for the entrapped glucose-1-phosphatase and K M = 0.35 mmol l-1 and k cat = 20.5 s-1 for the free enzyme. Complete conversion of phytate into one single myo-inositol pentakisphosphate isomer, identified as D-myo-inositol(1,2,4,5,6pentakis-phosphate, was shown to be feasible by using the enzyme-loaded alginate beads in batch operations. The entrapped enzyme showed a high operational stability by retaining almost full activity even after ten uses.

  14. DNA-based methodologies for the quantification of live and dead cells in formulated biocontrol products based on Pantoea agglomerans CPA-2.

    Science.gov (United States)

    Soto-Muñoz, Lourdes; Torres, Rosario; Usall, Josep; Viñas, Inmaculada; Solsona, Cristina; Teixidó, Neus

    2015-10-01

    Pantoea agglomerans strain CPA-2 is an effective biocontrol agent (BCA) against the major postharvest pathogens present on pome and citrus fruits. Dehydration, such as freeze-drying, spray-drying and fluidized bed drying is one of the best ways to formulate BCAs. In this work, the survival of CPA-2 cells after formulation was determined by dilution plating and molecular methods as qPCR alone and combined with a sample pretreatment with a propidium monoazide dye (PMA-qPCR) and they were used to calculate treatment concentrations in efficacy trials on postharvest oranges. Furthermore, no significant differences in CPA-2 survival were observed as determined by dilution plating and PMA-qPCR after both the freeze drying and fluidized bed drying processes; however, an interesting significant difference was observed in the spray dried product comparing all quantitative methods. A difference of 0.48 and 2.17 log10 CFU or cells g/dw was observed among PMA-qPCR with qPCR and dilution plating, respectively. According to our study, dilution plating was shown to be an unreliable tool for monitoring the survival of CPA-2 after spray drying. In contrast, the combination of PMA and qPCR enabled a quick and unequivocal methodology to enumerate viable and VBNC CPA-2 cells under stress-dried conditions. Efficacy trials showed that, after 3 days, spray drying formulation rehydrated with 10% non-fat skimmed milk (NFSM) was as effective as fresh cells to control Penicillium digitatum in oranges. Copyright © 2015 Elsevier B.V. All rights reserved.

  15. Biological control of Monilinia laxa and Rhizopus stolonifer in postharvest of stone fruit by Pantoea agglomerans EPS125 and putative mechanisms of antagonism.

    Science.gov (United States)

    Bonaterra, Anna; Mari, Marta; Casalini, Lucia; Montesinos, Emilio

    2003-07-15

    Treatment of stone fruits (apricot, peach and nectarine) with Pantoea agglomerans strain EPS125 decreased the incidence and diameter of lesions of brown rot caused by Monilinia laxa and soft rot caused by Rhizopus stolonifer. Root control was achieved on fruits either wounded and subsequently inoculated with the pathogens or non-wounded and naturally infected from orchards. The efficacy of biocontrol was dependent on the concentration of the biocontrol agent and pathogen. At medium to low pathogen dose, optimal EPS125 concentrations were above 10(7) CFU ml(-1). The median effective dose (ED(50)) of EPS125 was 4.5x10(4) in M. laxa and 2.2x10(5) CFU ml(-1) in R. stolonifer. However, EPS125 was more effective in M. laxa than in R. stolonifer as indicated by the ratio between ED(50) of the biocontrol agent and pathogen (K(z)/K(x)) which was 166 and 1263, respectively. Interactions between the strain EPS125 and the fruit surface, and M. laxa and R. stolonifer, were studied to determine the mechanisms of protection from postharvest rots. The strain EPS125 colonizes, grows and survives on stone fruit wounds. Significant inhibition of conidial germination and hyphal growth of R. stolonifer and M. laxa was achieved when the fungal and EPS125 cells were cocultivated on peel leachate or nectarine juice. However, no effect was observed when the antagonist and the pathogen cells were physically separated by a membrane filter which permits nutrient and metabolite interchange. Therefore, a direct interaction between the strain and the pathogen cells is necessary for antagonism, without a significant contribution of the production of antibiotic substances or nutrient competition. Preemptive exclusion by wound colonization and direct interaction with the pathogen is proposed as the mechanism of biocontrol.

  16. Modelos e experiências de competência em informação em contexto universitário

    Directory of Open Access Journals (Sweden)

    Fernanda Maria Melo Alves

    2014-12-01

    Full Text Available http://dx.doi.org/10.5007/1518-2924.2014v19n41p83 O artigo indica alguns estudos diacrônicos de revisão e de comparação sobre competência em informação a nível mundial e investiga alguns modelos em contexto universitário e suas respectivas aplicações, descrevendo experiências realizadas com estudantes, em diferentes universidades e países. Para tanto, realizou-se uma pesquisa bibliográfica e documental. Entre os resultados destaca-se a diversidade cronológica e geográfica dos modelos, bem como as suas diversas possibilidades de aplicação para a avaliação e o desenvolvimento da competência em informação de universitários.

  17. Paratuberculose em ruminantes no Brasil

    Directory of Open Access Journals (Sweden)

    Elise M. Yamasaki

    2013-02-01

    Full Text Available A paratuberculose ou doença de Johne é uma enterite granulomatosa causada por Mycobacterium avium subsp. paratuberculosis (Map e comumente afeta ruminantes domésticos, no entanto, pode infectar várias espécies de mamíferos. Está presente nos cinco continentes e é considerada endêmica em algumas regiões pela Organização Internacional de Epizootias (OIE. Pertence à lista de enfermidades notificáveis, que compreende as doenças transmissíveis de importância sócio-econômica e/ou em saúde-pública, cujo controle é necessário para o comércio internacional de animais e alimentos de origem animal. A importância da doença de Johne não se restringe somente aos prejuízos econômicos causados à indústria animal, mas também na possível participação do Map na íleocolite granulomatosa que afeta seres humanos, conhecida como doença de Crohn. No Brasil, a paratuberculose já foi descrita em diversas espécies de ruminantes e em vários estados. Embora os relatos naturais da enfermidade sejam pontuais, acredita-se na possibilidade da transmissão interespecífica e na disseminação do agente através da compra e venda de animais infectados. O objetivo deste artigo foi reunir as informações disponíveis referentes aos aspectos epidemiológicos, clínico-patológicos e laboratoriais da paratuberculose em bovinos, bubalinos, caprinos e ovinos no Brasil, e salientar a necessidade de implementação de medidas de controle sanitário da enfermidade no país, o que possibilitaria a melhoria da qualidade e valorização dos produtos de origem animal no mercado internacional.

  18. Gerenciamento de Projetos em Pesquisa e Desenvolvimento

    Directory of Open Access Journals (Sweden)

    Mauro Maia Laruccia

    2013-01-01

    Full Text Available Este trabalho tem por objetivo estudar o gerenciamento de projetos na área de Pesquisa e Desenvolvimento, em um cenário onde os avanços da tecnologia têm influenciado nas mais diversas áreas do conhecimento humano e passaram a exigir abordagens diferenciadas na forma de planejar, executar e controlar os projetos, para assegurar os resultados almejados pelas organizações. Avaliar as formas de conhecimento aplicadas ao gerenciamento de projetos e as interações de suas disciplinas apresenta-se como uma condição oportuna para compreensão e melhoria do processo de gerenciamento de projetos. Considerando que a interdisciplinaridade destaca-se na crítica e na busca de respostas aos limites do conhecimento e que rompe com o paradigma disciplinar de produção do conhecimento particionado, a análise da forma de conhecimento do gerenciamento de projetos pode contribuir para desvelar oportunidades e engendrar melhorias neste processo. Verifica-se que o gerenciamento de projeto tem evoluído continuamente e congrega diversas disciplinas do conhecimento nos modelos praticados. Atualmente, um dos modelos mais consagrados de gerenciamento de projetos é o Project Management Body of Knowledge (PMBOK, publicado pelo Project Management Institute (PMI, que é utilizado em mais de 185 países. Vale ressaltar, que a simples comunicação entre as disciplinas do gerenciamento de projetos não significa que estejamos aplicando necessariamente uma abordagem interdisciplinar. Analisar o gerenciamento de projetos na ótica da interdisciplinaridade pode contribuir para o aperfeiçoamento dos modelos de gerenciamento de projetos e consequentemente para a geração de melhores resultados para as organizações.DOI:10.5585/gep.v3i3.82

  19. Vulnerabilidades presentes no percurso vivenciado pelos pacientes com HIV/AIDS em falha terapêutica

    Directory of Open Access Journals (Sweden)

    Petra Kelly Rabelo de Sousa

    2013-04-01

    Full Text Available Um grande avanço no tratamento da AIDS deu-se com o surgimento da terapia antiretroviral. Entretanto, diversas vulnerabilidades podem estar presentes no percurso vivenciado pelos pacientes durante o tratamento. Objetivou-se analisar as vulnerabilidades dos pacientes com HIV/AIDS em falha terapêutica. Estudo qualitativo realizado com sete usuários com AIDS considerados em falha terapêutica, em uma Unidade de Serviço Ambulatorial Especializado em HIV/AIDS, em Fortaleza-CE. Para interpretação dos dados, utilizou-se uma técnica de análise de conteúdo. Nos discursos, percebeu-se que os depoentes enfrentavam conflitos na família e, no ambiente de trabalho, dificuldades de relacionamento com as pessoas e estigma. Observou-se que os pacientes haviam vivenciado diversas situações que os tornaram mais suscetíveis à infecção por HIV e ao adoecimento.

  20. Transtorno de humor bipolar: diversas apresentações de uma mesma doença Bipolar mood disorder: different occurrences of the same disease

    Directory of Open Access Journals (Sweden)

    Audrey Regina Magalhães Braga

    2008-04-01

    Full Text Available O presente relato tem por objetivo acompanhar a evolução de um caso que se apresentou como mutismo seletivo e evoluiu para transtorno de humor bipolar e chamar atenção para a diversidade de sintomas possíveis no transtorno de humor. Em geral, o mutismo seletivo tem início na idade pré-escolar, porém os sintomas chamam mais atenção na idade escolar. A importância do diagnóstico e tratamento precoces reside na prevenção das complicações, tais como distúrbios no desenvolvimento social e acadêmico e na auto-estima, além da possibilidade de evolução para outros transtornos de ansiedade. O transtorno de humor bipolar é uma doença mental caracterizada por variações extremas no humor. Na criança, prejudica o crescimento emocional e seu desenvolvimento. É confundido com transtorno de déficit de atenção/hiperatividade e transtornos de comportamento, tais como transtorno de conduta e desafiador opositivo. A paciente do sexo feminino, quando avaliada, estava com 5 anos e 9 meses de idade. Concluiu-se o diagnóstico como mutismo seletivo. Foi indicada psicoterapia, com orientações aos pais e à escola, e iniciado o uso de inibidores seletivos de recaptação da serotonina, com boa resposta aos sintomas de ansiedade, mas passou a apresentar piora significativa do comportamento. Foi levantada a hipótese diagnóstica de transtorno de humor bipolar de início precoce. Medicada com estabilizador, apresentou adequação na terceira medicação e evolução satisfatória. O mutismo seletivo, considerado um transtorno de ansiedade na infância, pode ser pródromo para outros quadros psiquiátricos na infância. Há necessidade que os pediatras, médicos que primeiro acessam essas crianças, assim como os psiquiatras da infância, estejam atentos à riqueza de sintomas que pode dar seguimento ao quadro.The purpose of the present case report is to follow the evolution of a child who presented selective mutism and evolved into bipolar

  1. Alcoolismo: Um problema social em assentamentos rurais

    Directory of Open Access Journals (Sweden)

    Marcelo Leles Romarco de Oliveira

    2012-06-01

    Full Text Available Este artigo procura fazer algumas reflexões a cerca do alcoolismo em assentamentos rurais, no município de Padre Bernardo-GO. O interesse em estudar esse tema foi a partir da observação de campo em diversas comunidades rurais do país, onde percebi que o uso do álcool trouxe sérios problemas para as famílias que têm algum membro que sofre do alcoolismo. Desta forma, os dados apresentados nesse trabalho são frutos de trabalhos de campo realizados entre os anos de 2001 e 2006. Como caminho para a coleta das informações optei pela observação e análise do dia-dia de um grupo de alcoólatras no assentamento Vereda I. Os resultados apresentaram que o álcool além de ser uma doença crônica, o seu uso é uma forma de fuga para os assentados. Igualmente, percebi que a ausência de políticas públicas que combata o alcoolismo corrobora para a gravidade da situação, culminando com agravamento das condições de vida do alcoólatra e de suas famílias.

  2. Self: um conceito em desenvolvimento

    Directory of Open Access Journals (Sweden)

    Lídia Suzana Rocha de Macedo

    2012-08-01

    Full Text Available Diversas definições de self coexistem nas teorias e práticas psicológicas. Essa variedade é resultante de bases epistemológicas a partir das quais se adotam estratégias diferentes para abordar e demarcar os limites do objeto em questão e descrevê-lo. Este estudo teve como objetivo oferecer uma revisão dos conceitos de self e uma reflexão sobre como esse conceito se articula nas diferentes abordagens teóricas da psicologia. Destaca-se que dilemas centrais à psicologia do desenvolvimento atravessam o conceito de self. Por essa razão, apresenta-se a tese de que, ao analisar cada definição de self, deve-se buscar responder como cada teoria colocou-se diante das dicotomias estabilidade versus transformação, específico versus universal e mundo interno versus mundo externo.

  3. DIARRÉIA NEONATAL DE ORIGEM BACTERIANA EM BOVINOS

    Directory of Open Access Journals (Sweden)

    Celso Pianta

    1993-04-01

    Full Text Available Diarréia neonatal em bovinos é um quadro complexo, normalmente responsável por altas taxas de morbidade e mortalidade. Os prejuízos são observados não somente nos gastos com a medicação, mas também na perda do animal em caso de morte e na redução do desenvolvimento e do desempenho do futuro animal adulto, comprometendo os índices de ganho de peso ou produção leiteira. A etiologia múltipla da enfermidade envolve a interação de diversas variáveis dos agentes, hospedeiro e meio ambiente. Nesta revisão são abordados os aspectos clínicos da colibacilose sanitário, a prevenção e o tratamento são também abordados.

  4. Lesões em tenistas amadores no Rio de Janeiro

    OpenAIRE

    Queiroz, Nelcimar Souza; Vigário, Patrícia dos Santos; Felicio, Lilian Ramiro; Mainenti, Míriam Raquel Meira

    2014-01-01

    INTRODUÇÃO: O treinamento desportivo em diversas modalidades está associado à presença de lesões, principalmente quando apresenta grande volume e intensidade. Poucos estudos nacionais investigaram a prevalência de lesão em tenistas, porém, as informações sobre a localização, o tipo e o mecanismo das lesões que acometem esse grupo de atletas são importantes para a prevenção e o tratamento de tais lesões.OBJETIVO: Verificar a frequência de lesão relatada por tenistas amadores no município do Ri...

  5. Algoritmos de agrupamento particionais baseados na Meta-heurística de otimização por busca em grupo

    OpenAIRE

    PACÍFICO, Luciano Demétrio Santos

    2016-01-01

    A Análise de Agrupamentos, também conhecida por Aprendizagem Não-Supervisionada, é uma técnica importante para a análise exploratória de dados, tendo sido largamente empregada em diversas aplicações, tais como mineração de dados, segmentação de imagens, bioinformática, dentre outras. A análise de agrupamentos visa a distribuição de um conjunto de dados em grupos, de modo que indivíduos em um mesmo grupo estejam mais proximamente relacionados (mais similares) entre si, enquanto ...

  6. Prevalência, fatores de risco e marcadores bioquímicos em cães com lama biliar diagnosticada por ultrassonografia

    OpenAIRE

    Priscila Secchi

    2011-01-01

    A lama biliar em cães é considerada um achado incidental, sendo frequentemente identificada durante ultrassonografias abdominais como sedimento ecogênico de baixa amplitude sem sombra acústica e com mobilidade gravidade dependente. Em humanos, a presença de lama biliar é considerada anormal e está associada a diversas situações clínicas e complicações como cálculos biliares, colangite e pancreatite aguda. Em cães sua importância clínica ainda é desconhecida. Os objetivos deste estudo foram es...

  7. Projeto e análise de moduladores sigma-delta em tempo contínuo aplicados à conversão AD

    OpenAIRE

    Paulo Cesar Comassetto de Aguirre

    2014-01-01

    Conversores analógico-digitais (ADCs) têm papel fundamental na implementação dos sistemas-em-chip, do inglês System-on-Chip (SoC), atuais. Em razão dos requisitos destes sistemas e dos compromissos entre as características fundamentais dos ADCs, como largura de banda, consumo de energia e exatidão, diversas topologias e estratégias para sua implementação em circuitos integrados (CIs) têm sido desenvolvidas através dos tempos. Dentre estas topologias, os conversores sigma-delta (SDC) têm se de...

  8. EFFECT OF DIFFERENT MULCHES ON THE CONTROL OF UNWISHED PLANTS IN MAIZE CROP (Zea mays EFEITO DE DIVERSAS ESPÉCIES DE COBERTURA MORTA SOBRE O CONTROLE DE PLANTAS DANINHAS DA CULTURA DO MILHO

    Directory of Open Access Journals (Sweden)

    Rogério de Araújo Almeida

    2007-09-01

    Full Text Available

    Researches of many South American countries point the zero tillage system of crop production for little farmers as a responsible factor for best soil and water conservation, less costs of production and best profits to improve the rural way of life. The treatments usually include the application of herbicides. To find agricultural systems that allow zero tillage without herbicide or with reduced usage has been considered a challenge by the researchers. This experiment was carried out in order to evaluate the effects of different mulches on the control of unwished plants in maize crop (<em>Zea> <em>mays>. Plants such as sunhemp (<em>Crotalaria> <em>juncea>, millet (<em>Pennisetum> <em>americanum> and sunflower (<em>Helianthus> <em>annus> L. cv. Stanzuela and V 2000 were grown in 100 m² areas. The sowing was made at the second April fortnight and, after sixty days, the zero tillage system with maize was established by using a manual planting machine. Just after, the plants grown in the plots were manually cut and used as mulch. The soil fertility was corrected according to the laboratorial analyses and the plant population adopted was around 60,000 plants per hectare. No weed control was made. At the maize flowering, the seed weeds grown in the plots were collected by using two samples of 1 m² plot (two replications. The plant material was dried till constant weight. Statistical analyses obtained from the data indicated the millet mulch as the best treatment to the weed control.

    Isolation and characterization of a ColE1-like plasmid from Enterobacter agglomerans with a novel variant of rom gene.

    Science.gov (United States)

    Mikiewicz, D; Wróbel, B; Wegrzyn, G; Płucienniczak, A

    1997-01-01

    Complete nucleotide sequence of a plasmid isolated from Enterobacter agglomerans has been determined. The plasmid, called pPIGDM1, consists of 2495 base pairs. The analysis of its nucleotide sequence suggested that pPIGDM1 may be a ColE1-like replicon. We confirmed this hypothesis by constructing a pPIGDM1-derived plasmid harboring the cat gene (pBW4), which could be introduced into Escherichia coli cells, and demonstrating that pBW4 cannot replicate in the absence of the polA function and that its copy number is significantly decreased in the pcnB mutant. Like some other ColE1-type replicons (e.g., pBR322), pPIGDM1-derived plasmids can be amplified both by chloramphenicol method and in isoleucine-starved relA mutants but not in relA+ bacteria. Inactivation of the putative rom gene by insertion of an amplicillin-resistance gene resulted in significant increase in pPIGDM1-derived plasmid copy number in E. coli-despite the fact that amino acid sequence of the putative RNA 1 modulator (Rom) protein is only 55.7% identical to the ColE1 analog. The pPIGDM1-derived rom-like coding sequence is also homologous to the rom-like gene present in the Proteus vulgaris plasmid pPvul. We suggest to group all these gene products into a new family called ROMS (RNA one modulators). Since a pPIGDM1-derived plasmid is compatible with other ColE1-like replicons (pMB1-, p15A, RSF1030-, and CloDF13-derived) in E. coli, one may consider pPIGDM1 as a progenitor of new cloning vehicles compatible with most (if not all) of currently used plasmid vectors. Moreover, this plasmid may serve as a source of the new rom-like gene coding for a protein useful in investigation of RNA-protein interactions. A role for the pPIGDM1 plasmid in the host strain is not known.

  9. Development of PMA real-time PCR method to quantify viable cells of Pantoea agglomerans CPA-2, an antagonist to control the major postharvest diseases on oranges.

    Science.gov (United States)

    Soto-Muñoz, Lourdes; Teixidó, Neus; Usall, Josep; Viñas, Inmaculada; Crespo-Sempere, Ana; Torres, Rosario

    2014-06-16

    Dilution plating is the quantification method commonly used to estimate the population level of postharvest biocontrol agents, but this method does not permit a distinction among introduced and indigenous strains. Recently, molecular techniques based on DNA amplification such as quantitative real-time PCR (qPCR) have been successfully applied for their high strain-specific detection level. However, the ability of qPCR to distinguish viable and nonviable cells is limited. A promising strategy to avoid this issue relies on the use of nucleic acid intercalating dyes, such as propidium monoazide (PMA), as a sample pretreatment prior to the qPCR. The objective of this study was to optimize a protocol based on PMA pre-treatment samples combined with qPCR to distinguish and quantify viable cells of the biocontrol agent P. agglomerans CPA-2 applied as a postharvest treatment on orange. The efficiency of PMA-qPCR method under the established conditions (30μM PMA for 20min of incubation followed by 30min of LED light exposure) was evaluated on an orange matrix. Results showed no difference in CFU or cells counts of viable cells between PMA-qPCR and dilution plating. Samples of orange matrix inoculated with a mixture of viable/dead cells showed 5.59log10 CFU/ml by dilution plating, 8.25log10 cells/ml by qPCR, and 5.93log10 cells/ml by PMA-qPCR. Furthermore, samples inoculated with heat-killed cells were not detected by dilution plating and PMA-qPCR, while by qPCR was of 8.16log10 cells/ml. The difference in quantification cycles (Cq) among qPCR and PMA-qPCR was approximately 16cycles, which means a reduction of 65,536 fold of the dead cells detected. In conclusion, PMA-qPCR method is a suitable tool for quantify viable CPA-2 cells, which could be useful to estimate the ability of this antagonist to colonize the orange surface. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. Objetos mediadores em museus

    Directory of Open Access Journals (Sweden)

    Inês Ferreira

    2015-02-01

    Full Text Available Um museu participativo incentiva a que a experiência da visita seja criativa e pessoal. Facilita a participação biográfica do visitante - com a sua experiência, conhecimento e interesses. Muitas exposições, porém, não promovem esse envolvimento pessoal. Neste artigo apresentamos uma revisão da literatura que indicia que a mediação com objetos mediadores facilita o envolvimento pessoal, a construção de conhecimento e a criatividade. O problema teórico que colocamos é se os museus mais tradicionais se podem abrir à participação e, neste contexto, o nosso objetivo é perceber que papel podem ter os objetos mediadores nesse processo. Discutimos o recurso a objetos mediadores como forma de adicionar múltiplos níveis de relação e modos de ver, potencial que consideramos subaproveitado em muitos museus. Começamos por apresentar o museu como espaço potencialmente participativo, que convida a um envolvimento ativo e crítico – ativo, porque o visitante é desafiado a fazer coisas; crítico, porque é estimulado a pensar, optar, discutir e refletir. Discutimos depois diferentes formas de usar objetos mediadores para incentivar o visitante a um envolvimento com os artefactos, recorrendo à memória, experiência, conhecimento e sentimentos. A participação biográfica potencia o cruzamento entre o mundo interior do visitante e os artefactos e conteúdos do museu. Esse espaço de cruzamento - inbetween - é o espaço da construção de conhecimento. Apresentamos diversas formas de um museu, mesmo não sendo interativo, se tornar mais criativo e participativo, o que pode contribuir para uma mudança nas práticas dos museus, nomeadamente na montagem e remodelação de exposições permanentes.

  11. Reinvenções e controvérsias: a necessidade da tradição e as disputas em torno da regulamentação da ayahuasca

    OpenAIRE

    Antunes, Henrique Fernandes

    2017-01-01

    Ayahuasca, um ser divino, uma planta mestra, enteógeno, alucinógeno, remédio, terapia, droga, ferramenta cognitiva, commodity, chá. As definições associadas à ayahuasca são quase tão diversas quanto seus usos. De fato, o uso da ayahuasca em todo o mundo é composto de diversas práticas - xamanismo indígena, grupos religiosos, uso terapêutico, grupos neoxamânicos associados ao movimento da nova era, formas particulares de turismo e empreendedorismo, etc. Tal variedade de práticas, usos e grupos...

  12. POR UM CURRÍCULO NA PERSPECTIVA DE UMA EDUCAÇÃO MAIS DIVERSA, DINÂMICA E DEMOCRÁTICA: debates atuais na Educação Básica e no Ensino Superior

    Directory of Open Access Journals (Sweden)

    Maria Zuleide da Costa Pereira

    2014-09-01

    Full Text Available A revista Espaço do Currículo elege no volume sete, segundo número de 2014 a temática: POR UM CURRÍCULO NA PERSPECTIVA DE UMA EDUCAÇÃO MAIS DIVERSA, DINÂMICA E DEMOCRÁTICA: debates atuais na Educação Básica e no Ensino Superior. Com isso, além de dar continuidade à política socializadora de levar ao grande público temáticas emergentes que, inquietam o contexto da Educação Básica e do Ensino Superior no Brasil, possibilita publicações voltadas para a divulgação de artigos científicos de autores de diferentes instituições e posicionamentos...

  13. Reference Gene Selection in the Desert Plant <em>Eremosparton songoricuem>m>

    Directory of Open Access Journals (Sweden)

    Dao-Yuan Zhang

    2012-06-01

    Full Text Available <em>Eremosparton songoricum em>(Litv. Vass. (<em>E. songoricumem> is a rare and extremely drought-tolerant desert plant that holds promise as a model organism for the identification of genes associated with water deficit stress. Here, we cloned and evaluated the expression of eight candidate reference genes using quantitative real-time reverse transcriptase polymerase chain reactions. The expression of these candidate reference genes was analyzed in a diverse set of 20 samples including various <em>E. songoricumem> plant tissues exposed to multiple environmental stresses. GeNorm analysis indicated that expression stability varied between the reference genes in the different experimental conditions, but the two most stable reference genes were sufficient for normalization in most conditions.<em> EsEFem> and <em>Esα-TUB> were sufficient for various stress conditions, <em>EsEF> and <em>EsACT> were suitable for samples of differing germination stages, and <em>EsGAPDH>and <em>Es>UBQ em>were most stable across multiple adult tissue samples. The <em>Es18Sem> gene was unsuitable as a reference gene in our analysis. In addition, the expression level of the drought-stress related transcription factor <em>EsDREB2em>> em>verified the utility of<em> E. songoricumem> reference genes and indicated that no single gene was adequate for normalization on its own. This is the first systematic report on the selection of reference genes in <em>E. songoricumem>, and these data will facilitate future work on gene expression in this species.

  14. DETERMINAÇÃO DE CORANTES SINTÉTICOS EM BEBIDAS ISOTÔNICAS EMPREGANDO EXTRAÇÃO EM FASE SÓLIDA DISPERSIVA E HPLC-UV/Vis

    OpenAIRE

    Luana Floriano

    2016-01-01

    As bebidas isotônicas são especialmente formuladas para pessoas que praticam atividade física, sendo compostas principalmente por carboidratos, eletrólitos e vitaminas. Os corantes sintéticos são aditivos alimentares comumente utilizados em bebidas isotônicas para aprimorar suas características de consumo e aparência. Além do menor custo de produção em relação aos corantes de origem natural, os corantes sintéticos apresentam diversas vantagens, tais como alta estabilidade à luz, oxigênio e pH...

  15. InvestigaÃÃo da aÃÃo central do carvacrol em modelos de ansiedade, depressÃo e convulsÃo em camundongos e possÃveis mecanismos farmacolÃgicos envolvidos.

    OpenAIRE

    Francisca Helvira Cavalcante FÃlix

    2010-01-01

    Carvacrol (5-Isopropil-2-metilfenol) Ã um monoterpeno fenÃlico presente nos Ãleos essenciais de diversas plantas. Ã o principal constituinte dos Ãleos essenciais de orÃgano e thyme. Este trabalho apresenta as aÃÃes comportamentais do carvacrol em modelos animais de ansiedade, depressÃo, sedaÃÃo e convulsÃo, tais como labirinto em cruz elevado (LCE), campo aberto, rota rod, tempo de sono induzido por pentobarbital, convulsÃo induzida por pentilenotetrazol, nado forÃado e suspensÃo da cauda. Ca...

  16. Desenvolvimento de Órgãos da Digestão e Rendimento de Carcaça de Frangos de Corte de Diversas Origens Genéticas Criados com Bebedouros Pendular e Nipple Development of Digestive Organs and Carcass Yield of Broilers from Diverse Genetic Origin Raised with Regular or Nipple Drinkers

    Directory of Open Access Journals (Sweden)

    LM Fernandes

    2002-01-01

    Full Text Available O uso de linhas genéticas de diferentes fontes comerciais em programas de reprodução de frangos de corte permite produzir gerações comerciais mais bem adaptadas a realidades diversas e otimizar os potenciais de desempenho do frango de corte com os da reprodutora. Entretanto, a prática predominante no Brasil é o uso de pais e mães de mesma origem. O uso de bebedouros tipo "nipple" vem sendo difundido com a perspectiva de gerar benefícios ainda não comprovados inteiramente na prática. Esse estudo avaliou a evolução dos órgãos da digestão, o desempenho vivo e o rendimento de carcaça e das partes de frangos de corte de diferentes cruzamentos entre reprodutores machos de diversos cruzamentos entre linhagens comerciais (R, I, S, e H com fêmeas também de origem variável (R, I, e H em sistemas de produção com bebedouros pendular ou nipple. As aves dos diferentes cruzamentos apresentaram curvas de crescimento com diferentes intensidades em momentos distintos, determinando desempenhos em peso vivo sempre superiores para o cruzamento RR. O rendimento de peito, entretanto, foi maior para o cruzamento IH. O cruzamento SH, de mais baixo peso corporal, evidenciou rendimentos de carcaça inferiores a todos os demais. O tipo de bebedouro não influenciou nenhum resultado de desempenho vivo ou de rendimento de carcaça, independentemente da linhagem. Os órgãos da digestão apresentaram proporções do peso vivo decrescentes com a idade, mas não evidenciaram efeito de cruzamento entre linhagens ou tipo de bebedouro.Breeding programs using parents from different genetic companies allow the production of commercial generations of broilers directed to attend diverse market situations, but they also serve to optimize the performance of the breeder female. However, this is not a current practice in Brazil where most of the commercial broilers being raised are originated from crosses within the same genetic company. The use of nipple drinkers in

  17. Caracterização molecular de Cryptosporidium spp. em criações comerciais brasileiras de coelhos

    OpenAIRE

    Heker,M.M.; Nakamura,A.A.; Meireles,M.V.

    2016-01-01

    RESUMO A criptosporidiose é uma importante zoonose que pode ser transmitida por meio de alimentos, água de bebida e por contato com animais e pessoas infectadas. Além disso, trata-se de uma enfermidade clínica ou subclínica frequente em diversas espécies de animais, incluindo coelhos domésticos. O objetivo deste estudo foi determinar a ocorrência de Cryptosporidium spp., realizar sua classificação molecular e relacionar a presença do parasito às diferentes fases de criação em 21 criações come...

  18. Neuropatia periférica em pacientes com síndrome antifosfolípide primária

    OpenAIRE

    Mário Sérgio Ferreira Santos

    2009-01-01

    O envolvimento do sistema nervoso periférico em diversas doenças auto-imunes é bem estabelecido. No entanto, não existem estudos, com desenho metodológico apropriado, que tenham investigado a relação entre síndrome antifosfolípide primária (SAFP) e neuropatia periférica. Nosso objetivo nesse trabalho foi investigar a ocorrência de neuropatia periférica em pacientes com SAFP. Vinte e seis pacientes com SAFP (critérios de Sapporo) e vinte controles, saudáveis, pareados por sexo e idade, foram r...

  19. Ceratoconjuntivite seca associada a degeneração de retina e esclerose nuclear em cadela. Relato de Caso

    OpenAIRE

    Souza, Ana Carolina Henrique de; Universidade Estadual do Ceará.; Cunha, Duanny Murinelly de Souza; Universidade Estadual do Ceará.; Martins, Patricia Lustosa; Universidade Estadual do Ceará.; Mello, Mirza; Faculdade de Veterinária, Universidade Estadual do Ceará ,UECE, Fortaleza, CE; Costa, Paula Priscila Correia; Faculdade de Veterinária, Universidade Estadual do Ceará ,UECE, Fortaleza, CE

    2016-01-01

    Diversas alterações na retina e no cristalino ocorrem com o envelhecimento, culminando em degenerações de retina e esclerose nuclear para os quais não há tratamento. A ceratoconjuntivite seca é uma patologia imuno-mediada, mais em animais idosos, caracterizada por uma inflamação exarcebada nas glândulas lacrimais, reduzindo a produção de lágrimas. A sintomatologia das degenerações de retina é uma cegueira, enquanto na esclerose nuclear apenas uma opacidade no cristalino é observada. A sintoma...

  1. La sal en el queso: diversas interacciones.

    OpenAIRE

    Juan Sabastián Ramírez-Navas; Jessica Aguirre-Londoño; Víctor Alexander Aristizabal-Ferreira; Sandra Castro-Narváez

    2016-01-01

    El objetivo de este trabajo fue analizar el efecto de la sal sobre algunas propiedades físicas del queso, su interacción con los componentes del queso, y el efecto del contenido de sodio sobre la salud de los consumidores. La sal es un ingrediente importante, ya que determina en gran parte la calidad del producto y la aceptación del consumidor. El salado del queso tiene in uencia en la calidad debido a sus efectos sobre la composición, el crecimiento microbiano y la actividad enzimática. Ejer...

  2. La sal en el queso: diversas interacciones.

    Directory of Open Access Journals (Sweden)

    Juan Sabastián Ramírez-Navas

    2016-12-01

    Full Text Available El objetivo de este trabajo fue analizar el efecto de la sal sobre algunas propiedades físicas del queso, su interacción con los componentes del queso, y el efecto del contenido de sodio sobre la salud de los consumidores. La sal es un ingrediente importante, ya que determina en gran parte la calidad del producto y la aceptación del consumidor. El salado del queso tiene in uencia en la calidad debido a sus efectos sobre la composición, el crecimiento microbiano y la actividad enzimática. Ejerce una in uencia signi cativa sobre la reología y textura, así como en la maduración, principalmente a través de sus efectos sobre la actividad del agua. Los niveles de sal en queso van desde aproximadamente 0,6% p/p hasta aproximadamente 7% p/p. Debido a que el consumo de queso está aumentando en todo el mundo, se debe dar importancia a la reducción de la sal sin afectar su consumo. Entre las estrategias que se han planteado con tal n está la sustitución parcial de la sal por otros compuestos. Pero el inconveniente de la sustitución del NaCl es su efecto sobre las propiedades sensoriales, la composición química, la proteólisis y la textura del queso. Otra interesante alternativa para el reemplazo del NaCl, es el uso de la tecnología de membranas para obtener permeado rico en sales provenientes del lactosuero; la adición de estas sales en la elaboración quesera, produce quesos bajos en sodio, con buena textura.

  3. Imigração em Portugal

    Directory of Open Access Journals (Sweden)

    Igor José de Renó Machado

    2006-08-01

    Full Text Available NESTE ARTIGO pretendo refletir sobre as imigrações brasileiras para Portugal e suas relações com categorias étnicas portuguesas. Levando em consideração que a localiza��ão da imigração brasileira numa hierarquia das alteridades portuguesa pressupõe uma etnização do conjunto da população migrante brasileira, procurarei demonstrar como o que se esconde sob o rótulo etnicizado de "brasileiros em Portugal" é uma população diversa e extremamente variada em suas formas de construção de identidades. Esboçarei, ainda, uma análise sobre as conseqüências políticas do discurso étnico-político no que se refere à cidadania e integração da população imigrante brasileira em Portugal.THIS PAPER is an excogitation on Brazilian immigration to Portugal and on the relationship between the immigrants and various Portuguese ethnic categories. Seeing that the sites chosen by Brazilian immigrants from among a hierarchy of Portuguese alterities presume an ethnization of the entire Brazilian migrant population, I have attempted to demonstrate that, hidden beneath the ethnicized label of "Brazilians in Portugal", one finds a diverse and extremely varied population in terms of how they build their identity. In addition, I have made a concise analysis of the political consequences of the ethnical-political discourse regarding the citizenship and integration of the Brazilian immigrant population in Portugal.

  4. Synthesis, Crystal Structure and Luminescent Property of Cd (II Complex with <em>N-Benzenesulphonyl-L>-leucine

    Directory of Open Access Journals (Sweden)

    Xishi Tai

    2012-09-01

    Full Text Available A new trinuclear Cd (II complex [Cd3(L6(2,2-bipyridine3] [L =<em> Nem>-phenylsulfonyl-L>-leucinato] has been synthesized and characterized by elemental analysis, IR and X-ray single crystal diffraction analysis. The results show that the complex belongs to the orthorhombic, space group<em> Pem>212121 with<em> aem> = 16.877(3 Å, <em>b> em>= 22.875(5 Å, <em>c em>= 29.495(6 Å, <em>α> em>= <emem>= <emem>= 90°, <em>V> em>= 11387(4 Å3, <em>Z> em>= 4, <em>Dc>= 1.416 μg·m−3, <emem>= 0.737 mm−1, <em>F> em>(000 = 4992, and final <em>R>1 = 0.0390, <em>ωR>2 = 0.0989. The complex comprises two seven-coordinated Cd (II atoms, with a N2O5 distorted pengonal bipyramidal coordination environment and a six-coordinated Cd (II atom, with a N2O4 distorted octahedral coordination environment. The molecules form one dimensional chain structure by the interaction of bridged carboxylato groups, hydrogen bonds and p-p interaction of 2,2-bipyridine. The luminescent properties of the Cd (II complex and <em>N-Benzenesulphonyl-L>-leucine in solid and in CH3OH solution also have been investigated.

  5. CONTAMINANTES AMBIENTAIS PRESENTES EM ATIVIDADES DIDÁTICO-EXPERIMENTAIS EM CURSOS DE FARMÁCIA: IDENTIFICAÇÃO E PROPOSTA DE GESTÃO

    OpenAIRE

    Paiva, Cláudia Cristina Sousa de

    2017-01-01

    Os resíduos gerados nas diversas atividades humanas devem ser tratados e dispostos adequadamente, o que acarreta um grande desafio para a administração pública e privada, principalmente nos centros urbanos. Os resíduos gerados e a quantidade de água utilizada nos processos de produção e de higienização do material utilizado nas aulas de laboratório de disciplinas como Farmacotécnica e Cosmetologia em Instituições de Ensino Superior (IES) necessita ser identificado e quantificad...

  6. Molecular Cloning and Functional Analysis of Three <em>FLOWERING LOCUS T (FTem> Homologous Genes from Chinese <em>Cymbidium>

    Directory of Open Access Journals (Sweden)

    Jaime A. Teixeira da Silva

    2012-09-01

    Full Text Available The <em>FLOWERING LOCUS Tem> (<em>FT> gene plays crucial roles in regulating the transition from the vegetative to reproductive phase. To understand the molecular mechanism of reproduction, three homologous <em>FT> genes were isolated and characterized from <em>Cymbidium sinenseem> “Qi Jian Bai Mo”, <em>Cymbidium goeringiiem> and <em>Cymbidium ensifoliumem> “Jin Si Ma Wei”. The three genes<em> em>contained 618-bp nucleotides with a 531-bp open reading frame (ORF of encoding 176 amino acids (AAs. Alignment of the AA sequences revealed that CsFT, CgFT and CeFT contain a conserved domain, which is characteristic of the<em> em>PEBP-RKIP superfamily, and which share high identity with FT of other plants in GenBank: 94% with OnFT<em> em>from <em>Oncidium em>Gower Ramsey, 79% with Hd3a from <em>Oryza sativaem>, and 74% with FT from <em>Arabidopsis thalianaem>. qRT-PCR analysis showed a diurnal expression pattern of <em>CsFT>, <em>CgFT> and <em>CeFT> following both long day (LD, 16-h light/8-h dark and short day (SD, 8-h light/16-h dark treatment. While the transcripts of both <em>CsFT em>and <em>CeFT em>under LD were significantly higher than under SD, those of <em>CgFT em>were> em>higher under SD. Ectopic expression of <em>CgFT> in transgenic <em>Arabidopsis> plants resulted in early flowering compared to wild-type plants and significant up-regulation of <em>APETALA1em> (<em>AP1em> expression. Our data indicates that CgFT is a putative phosphatidylethanolamine-binding protein gene in <em>Cymbidium> that may regulate the vegetative to reproductive transition in flowers, similar to its <em>Arabidopsis> ortholog.

  7. Valorização de beleza e inteligência por adolescentes de diferentes classes sociais Valorización de belleza e inteligencia por adolescentes de diversas clases sociales Adolescents of different social classes giving value of beauty and intelligence

    Directory of Open Access Journals (Sweden)

    Gizelle Regina Gomes

    2007-08-01

    Full Text Available Participaram do estudo 180 adolescentes (90M e 90F com idades variando entre 15 e 17 anos, alunos do ensino médio, pertencentes a diferentes classes sociais. Foram comparados gêneros e classes sócio-econômicas a respeito dos valores de beleza e de inteligência que adolescentes atribuem a si próprios e, numa situação hipotética e de forma mutuamente exclusiva, qual desses atributos foram mais valorizados para si mesmos e para possíveis parceiros. Os resultados mostraram que os rapazes de classe alta atribuíram-se maiores notas de inteligência. Adolescentes de ambos sexos pertencentes à classe baixa gostariam de possuir um maior nível de inteligência em detrimento da beleza, enquanto que adolescentes de classe alta preferem o equilíbrio entre beleza e inteligência. As moças valorizam mais a inteligência em seus parceiros que os rapazes, os quais valorizam mais a beleza em suas parceiras.Participaran del estudio 180 jóvenes (90 varones y 90 mujeres con edades entre 15 y 17 años, alumnos de la educación secundaria, pertenecientes a diversas clases sociales. Fueron comparados géneros de clases socio-económicas respecto a valores de belleza y de inteligencia que los jóvenes se atribuyen a sí mismos y, en una situación hipotética y de forma mutuamente exclusiva, cuál de estas cualidades fueron más valorizadas por sí mismos y por posibles compañeros. Los resultados mostraron que los jóvenes de clase alta se atribuyen mayores notas a la inteligencia; a adolescente de ambos sexos, pertenecientes a la clase baja les gustaría tener un nivel mayor de inteligencia en detrimento de la belleza, mientras que los adolescentes de clases altas prefieren el equilibrio entre belleza e inteligencia. Las mujeres jóvenes valorizan más la inteligencia de sus compañeros que los varones, los cuales valorizan más la belleza en sus compañeras.The study sample comprised 180 adolescents aged 15 to 17 years, being 90 males and 90 females

  8. Uso do conceito de aprendizagem em estudos relacionados ao trabalho e organizações

    Directory of Open Access Journals (Sweden)

    Francisco Antonio Coelho Junior

    2008-01-01

    Full Text Available A literatura em comportamento organizacional apresenta diversos estudos referenciando o conceito aprendizagem aplicado no âmbito das organizações. Porém, verifica-se o uso pouco crítico ou sistemático deste conceito. O objetivo do presente artigo consiste em realizar uma análise conceitual do termo aprendizagem, justificando sua transposição para estudos organizacionais. Aplicaram-se técnicas de análise conceitual propostas por Ryle. Como resultado, verificou-se que a natureza multifacetada do conceito de aprendizagem favorece o advento das diversas definições associadas a ele. Espera-se que haja maior sistematicidade acerca do uso do termo aprendizagem aplicado em estudos organizacionais, considerando seus níveis de análise e as dimensões presentemente discutidas.

  9. Articulações entre o sensível e a linguagem em Lavoura arcaica

    Directory of Open Access Journals (Sweden)

    Renato Cury Tardivo

    2010-08-01

    Full Text Available Literatura e cinema são dois modos de produção estética diferentes que encontram articulações claras em diversas produções culturais contemporâneas. Exploraremos a obra Lavoura arcaica, de Raduan Nassar, em sua intersecção com o filme de mesmo nome, dirigido por Luiz Fernando Carvalho. Para tanto nos aproximamos das concepções filosóficas de Merleau-Ponty e Bakhtin, cujas ideias sustentam uma dimensão de tensão inerente à relação comunicativa/perceptiva. A análise das obras selecionadas procura explicitar o percurso de mudança na trajetória das relações humanas ao se tentar integrar a diversidade de possibilidades expressivas em um campo de sentido.

  10. NOS LIMIARES DA LITERATURA: HISTÓRIAS EM QUADRINHOS, ADAPTAÇÕES E OUTRAS ARTES

    OpenAIRE

    Miranda, Déborah Alves; Pinheiro-Mariz, Josilene

    2015-01-01

    Resumo: As fronteiras entre a arte literária e outras artes é uma significante questão que vem sendo fonte de debates na academia, tanto no domínio das Letras, quanto no das Artes. Tal desconforto se dá diante de leituras de clássicos de obras literárias adaptadas para diversas outras mídias e, muito especialmente, das histórias em quadrinhos. Portanto, aqui discutimos sobre a leitura literária e a sua relação com as histórias em quadrinhos. Em se tratando de quadrinhos “nascidos” como obras ...

  11. Armazenamento em sistema a frio de sementes de soja tratadas com fungicida

    OpenAIRE

    Cardoso, Paulo César; Baudet, Leopoldo; Peske, Silmar Teichert; Lucca Filho, Orlando Antonio

    2004-01-01

    O objetivo do presente trabalho foi avaliar a qualidade fisiológica e sanitária das sementes de soja, tratadas com fungicida, armazenadas em um sistema de armazenamento a frio. As sementes, tratadas com o fungicida Difenoconazole na dose de 0,05g i.a./kg de sementes, foram armazenadas na porção superior de duas pilhas de 1.200 sacos cada, no 23° lastro (penúltimo), em diversas posições em relação ao duto central de aeração do sistema a frio. Durante um período de oito meses, as sementes foram...

  12. BOOM AGRICOLA NA AMAZONIA BRASILEIRA E AS MUDANÇAS ESTRUTURAIS EM ANDAMENTO

    OpenAIRE

    de Mesquita, Benjamin Alvino

    2012-01-01

    Objetivo desta comunicação é apontar as mudanças estruturais em curso na Amazônia no contexto de crescimento agrícola, capitaneado pela produção de soja e carne, em que Amazônia atualmente se insere. Entre a ultima e atual década ocorreu importantes transformações na agricultura brasileira, e, na Amazônia. Essas mudanças não se restringem apenas aos aspectos da produção, elas se espraia em outras direções, com destaque para as questões estruturais,acarretando problemas de ordem diversa, parti...

  13. <em>α>-Glucosidase Inhibitory Constituents from <em>Acanthopanax senticosusem> Harm Leaves

    Directory of Open Access Journals (Sweden)

    Hai-Xue Kuang

    2012-05-01

    Full Text Available A new triterpene glycoside, 3-<em>O-[(α>-L-rhamnopyranosyl(1→2]-[<em>β>-D-glucuronopyranosyl-6-<em>O>-methyl ester]-olean-12-ene-28-olic acid (1 and a new indole alkaloid, 5-methoxy-2-oxoindolin-3-acetic acid methyl ester (5 were isolated from the leaves of <em>Acanthopanax senticosusem> Harms along with six known compounds. The structures of the new compounds were determined by means of 2D-NMR experiments and chemical methods. All the isolated compounds were evaluated for their glycosidase inhibition activities and compound 6 showed significant <em>α>-glucosidase inhibition activity.

  14. O estresse ocupacional e a síndrome do esgotamento profissional (burnout) em trabalhadores da indústria do petróleo: uma revisão sistemática

    OpenAIRE

    Dias, Fernanda Monteiro; Santos, Jacqueline Fernandes de Cintra; Abelha, Lucia; Lovisi, Giovanni Marcos

    2016-01-01

    Resumo Introdução: na indústria do petróleo, os trabalhadores atuam em diversas áreas e nas mais adversas condições de trabalho, o que pode resultar em agravos como o estresse ocupacional e o burnout. Objetivo: investigar a associação entre o ambiente de trabalho na indústria do petróleo e o estresse ocupacional e burnout nos trabalhadores, assim como, fatores psicossociais e sintomas físicos associados. Métodos: revisão sistemática de literatura realizada em nove bases de dados bibliog...

  15. Uso de banda dendrométrica na definição de padrões de crescimento individual em diâmetro de árvores da bacia do Rio Cuieiras.

    OpenAIRE

    SILVA, R. P. da; NAKAMURA, S.; AZEVEDO, C. P. de; CHAMBERS, J.; ROCHA, R. de M.; PINTO, A. C. M.; SANTOS, J. dos; HIGUCHI, N.

    2003-01-01

    Este trabalho tem como objetivo analisar padrões de crescimento individual de diversas árvores que ocorrem em duas toposseqüências (direções Norte-Sul e Leste-Oeste), de uma amostra representativa da floresta de terra-firme na Amazônia Central. Foram selecionados de forma aleatória, aproximadamente, 300 indivíduos, sendo 150 em cada toposseqüência, distribuídos em mesmas proporções nas três classes topográficas (p...

  16. Efeitos da facilitação neuromuscular proprioceptiva na estabilidade postural e risco de quedas em pacientes com sequela de acidente vascular encefálico: estudo piloto

    OpenAIRE

    Lacerda, Natália Noman de; Gomes, Érika Baptista; Pinheiro, Hudson Azevedo

    2013-01-01

    O controle do tronco é uma habilidade motora básica necessária para executar diversas tarefas funcionais, e é deficiente em pacientes que sofreram acidente vascular encefálico (AVE). Objetivo: Avaliar o efeito do método facilitação neuromuscular proprioceptiva (PNF) na estabilidade postural e risco de quedas em pacientes com sequela de AVE. Metodologia: Foi realizado estudo de intervenção que consistiu em treinamento da estabilidade postural por meio de um protocolo fixo constituído por 5 exe...

  17. Variabilidade sazonal dos constituintes da própolis vermelha e bioatividade em Artermia salina

    Directory of Open Access Journals (Sweden)

    Lívio César Cunha Nunes

    Full Text Available A própolis é uma substância resinosa coletada pelas abelhas de diversas partes das plantas. Sua composição depende da época, vegetação e local de coleta. Apresenta diversas atividades biológicas como antimicrobiana, antioxidante, antitumoral, dentre outras. Foi realizado estudo da variabilidade sazonal, nos meses de fevereiro, junho e outubro de 2006, dos constituintes voláteis da própolis vermelha de Pernambuco através da extração por headspace dinâmico e identificação por cromatografia gasosa acoplada com espectrometria de massas (CG-EM. Foram identificados 34 constituintes voláteis, sendo monoterpenos e monoterpenóides, sesquiterpenos e sesquiterpenóides, fenilpropanóides, aldeídos, cetonas e η-alcanos. Os constituintes majoritários foram o trans-anetol, α-copaeno e o metil cis-isoeugenol. Também foi realizado o perfil fitoquímico por cromatografia em camada delgada (CCD, através da qual os constituintes fenólicos foram identificados como majoritários. Com o extrato bruto metanólico da própolis, realizou-se o ensaio de letalidade em Artemia salina, que demonstrou DL50 de 18,9 µg/mL, sugerindo uma possível atividade antitumoral.

  18. Análisis de la relación entre ONG locales e internacionales para afianzar procesos de luchas políticas. Caso de estudio: Colombia Diversa y Human Rights Watch con respecto a la población LGBT entre 2004 – 2008

    OpenAIRE

    Barco López, Juan Sebastián

    2009-01-01

    Esta monografía pretende establecer en que medida la relación entre ONG locales e internacionales ha logrado afianzar procesos de luchas políticas de la población LGBT a partir del estudio de la relación entre Colombia Diversa y Human Rights Watch. Los propósitos particulares que persigue la investigación giran en torno a tres puntos: primero, analizar el surgimiento y la consolidación de Colombia Diversa y el papel de las ONG en el sistema internacional; segundo, analizar la relación entre O...

  19. Representações em A Queda e Eu Fui Secretária de Hitler

    Directory of Open Access Journals (Sweden)

    Spuldar, Rafael

    2005-01-01

    Full Text Available O fim do Terceiro Reich serve tanto de objeto de debate intenso no campo da História quanto como tema de diversas produções cinematográficas. Recentemente, dois longas-metragens alemães utilizaram abordagens completamente díspares para retratar o mesmo depoimento de uma testemunha ocular daquele período. A proposta do presente trabalho é reconhecer em cada filme modelos de representação cinematográfica, tendo como parâmetro as perspectivas de três diferentes autores sobre esta noção.

  20. Violência obstétrica em maternidades públicas do estado do Tocantins

    OpenAIRE

    Guimarães, Liana Barcelar Evangelista; Jonas, Eline; Amaral, Leila Rute Oliveira Gurgel do

    2018-01-01

    Resumo: Este artigo apresenta os resultados de uma pesquisa realizada em 14 maternidades públicas do Estado do Tocantins onde foram entrevistadas 56 mulheres por meio de entrevista semiestruturada. O estudo teve como objetivo identificar as percepções das mulheres sobre violência obstétrica no processo de parto. A percepção das mulheres sobre violência obstétrica apresentou-se relacionada à falta de qualidade e acolhimento na assistência, destacando a ocorrência de expressões diversas de viol...

  1. Complexidade e customização em massa na arquitetura contemporânea

    OpenAIRE

    Morais, Helen Rachel Aguiar

    2011-01-01

    A arquitetura contemporânea em diversas partes do mundo tem sido produzida com recursos computacionais, o que tem permitido a construção de edifícios com formas complexas. O uso de tecnologia CAD/CAM permite obter menores custos na fabricação de componentes únicos e diferenciados na construção de edificações, com maior rapidez, precisão e controle. De um modo geral o uso de CAD na elaboração de projetos é amplamente conhecido entre os arquitetos e engenheiros. Sabemos que CAD/CAM na fabric...

  2. A Reportagem Jornalistica e a Memória - Histórias em Movimento

    Directory of Open Access Journals (Sweden)

    Jerusa de Oliveira Michel

    2016-03-01

    Full Text Available O trabalho analisa a partir de diferentes campos do conhecimento: antropologia, etnografia, etnicidade, território, história e memória em suas diversas abordagens, e o gênero jornalístico reportagem, a perspectiva de construção de conhecimento interdisciplinar, identificando os pontos de convergência entre os campos. Utiliza metodologia etnográfica/interdisciplinar, focando o trabalho da jornalista Eliane Brum à luz do referencial teórico construído.

  3. Efeito da terapia ultrassônica de baixa intensidade em cultura celular de fibroblastos

    OpenAIRE

    Oliveira, Priscila Daniele de; Oliveira, Deise A. A. Pires; Martinago, Cintia Cristina; Frederico, Regina Célia Poli; Soares, Cristina Pacheco; Oliveira, Rodrigo Franco de

    2015-01-01

    Dentro da prática fisioterápica verifica-se a ampla utilização do ultrassom terapêutico para tratamento das diversas afecções musculoesqueléticas. O objetivo deste estudo foi avaliar o efeito da irradiação Ultrassônica de Baixa Intensidade, com diferentes regimes de pulsos e intensidade, em cultura celular de fibroblastos L929 (ATCC CCL-1 NCTC), de modo a verificar a viabilidade celular e definir parâmetros de dosimetria. Para isso, utilizou-se a aplicação de ultrassom pulsado, com frequência...

  4. Encontraria a literatura? As buscas pela Maga em Rayuela, de Julio Cortázar

    OpenAIRE

    DADALTO, W.

    2010-01-01

    Rayuela, de Julio Cortázar, empreende uma busca por uma literatura que seja capaz de promover o encontro com uma outra realidade possível, questionando assim os valores estéticos, cognitivos e sociais do Ocidente. A Maga, que se apresenta em diversas outras mulheres do romance, é a melhor representação dessa busca; por constituir-se enquanto poesia, a Maga está ligada a personagens femininas de outras obras literárias renovadoras, tanto de outros escritores quanto do próprio Cortázar. Horacio...

  5. Marcas teatrais: artimanhas discursivas em narrativas de Sérgio Sant'Ana

    OpenAIRE

    Meira, Ana Caroline de Oliveira

    2014-01-01

    Esta dissertação apresenta um estudo sobre o escritor carioca Sérgio Sant Anna (1941), dono de um projeto literário complexo e inovador que acaba por lhe garantir um lugar de destaque dentre os grandes nomes da literatura brasileira contemporânea. Tomadas como ―desarmônicas‖ em relação às classificações de gêneros estabelecidas pelo cânone literário, suas obras, pautadas na inventividade e na experimentação, se constroem a partir de diálogos estabelecidos com as diversas formas de...

  6. Simulação in vitro do processo digestivo de Patulina em sumos de fruta

    OpenAIRE

    Ferreira, Mariana Marques da Costa, 1991-

    2014-01-01

    Tese de mestrado. Biologia (Biologia Humana e Ambiente). Universidade de Lisboa, Faculdade de Ciências, 2014 Os géneros alimentícios podem apresentar contaminantes de diversas origens e com diferentes toxicidades, podendo representar um perigo para a saúde humana. As micotoxinas são metabolitos produzidos por fungos capazes de induzir efeitos carcinogénicos e imunossupressores, podendo constituir um problema grave para a saúde pública. A patulina, uma micotoxina presente essencialmente em ...

  7. Efeitos do glyphosate e do fósforo em eucalipto

    OpenAIRE

    Pereira, Fernanda Campos Mastrotti [UNESP

    2016-01-01

    A cultura do eucalipto apresenta uma grande importância no cenário econômico nacional e mundial, oferecendo uma vasta gama de produtos florestais. No entanto, a disponibilidade de fósforo nos solos raramente é adequada para o crescimento, desenvolvimento e produtividade das plantas de eucalipto, bem como para diversas outras culturas. A presença de plantas daninhas em eucaliptais é outro fator limitante, e para o manejo da comunidade infestante, glyphosate é o herbicida mais utilizado. As pla...

  8. Hidrocefalia infantil resultados tardios em 102 pacientes e importância da neuro -sonografia

    OpenAIRE

    Machado, Hélio R.; Martelli, Nelson; Assirati Jr, João A.; Colli, Benedito O.; Carlotti Jr, Carlos G.; Boullosa, José L. R.; Funayama, Carolina; Machado, Yara M. Nunes; Martins, M. Angélica O.; Almeida, Sueli

    1990-01-01

    Os autores apresentam sua experiência em 102 crianças com hidrocefalia de diversas etiologias, acompanhadas por 5 1/2 anos. Cerca de 80% dessas crianças tinham idade inferior a dois anos, o que possibilitou seu acompanhamento com a ultra-sonografia craniana (4,4 exames por paciente). Os resultados a longo prazo são comparáveis aos obtidos na literatura com redação ao número de procedimentos por paciente (1,66), índice de infecqões (5,2%), mortalidade (6,8%) e desenvolvimento intelectual. A ul...

  9. Sofrimento e qualidade de vida em doentes com cancro no HDES, E.P.E.

    OpenAIRE

    Morins, Gonçalo João dos Reis, 1980-

    2009-01-01

    Tese de mestrado, Cuidados Paliativos, 2009, Faculdade de Medicina, Universidade de Lisboa Os cuidados paliativos visam melhorar a qualidade de vida dos doentes através da prevenção e alívio do sofrimento. Ao longo da minha vivência profissional com doentes oncológicos diversas inquietações foram surgindo, pretendendo com este trabalho encontrar respostas para estas. O presente trabalho intitulado Sofrimento e Qualidade de Vida em doentes com cancro no HDES, E.P.E. , tem como objectivos a...

  10. Uma revisão de abordagens genético-difusas para descoberta de conhecimento em banco de dados

    Directory of Open Access Journals (Sweden)

    Wesley Romão

    2000-05-01

    Full Text Available O processo geral de descoberta de conhecimento em banco de dados é composto por diversas etapas, destacando-se a de Mineração de Dados (MD. As principais tarefas de MD são associação, agrupamento e descoberta de regras de classificação. A tarefa de classificação pode ser realizada por algoritmos convencionais (e.g., estatísticos ou por métodos de inteligência artificial (e.g., redes neurais, algoritmos evolucionários, etc.. Nesta pesquisa o interesse é revisar algumas abordagens que utilizam algoritmos genéticos (AG em combinação com conjuntos difusos (CD de forma híbrida para realizar busca em espaços grandes e complexos. Este artigo mostra diversas abordagens híbridas (AG+CD, desenvolvidas para descoberta de regras de classificação, disponíveis na literatura e indica a possibilidade de adaptação das mesmas na descoberta de conhecimento em banco de dados de Ciência e Tecnologia (C&T

  11. Presen?a de Aloanticorpos Eritrocit?rios em gestantes Rh negativo, atendidas na Funda??o de Hematologia e Hemoterapia do Amazonas (Hemoam).

    OpenAIRE

    Cavalcante, Francimary de Oliveira

    2005-01-01

    A presen?a de aloanticorpos irregulares eritrocit?rios da classe G, na circula??o materna, pode causar serias conseq??ncias ao neonato. A aloimunizacao pode ocorrer em diversas situa??es, consideradas de risco, como partos com incompatibilidade sangu?nea, aborto, amniocentese, transfus?es sangu?nea, dentre outras. Neste estudo foi verificada a presen?a de aloanticorpos circulantes regulares e irregulares, em mulheres atendidas no Programa de Gestantes Rh Negativo (PGRhN) da Funda??o de Hemato...

  12. O enfermeiro como promotor da segurança infantil na administração da terapêutica em ambulatório

    OpenAIRE

    Porfírio, Susana de Oliveira Rocha

    2016-01-01

    O presente relatório foi desenvolvido no âmbito do Curso de Mestrado em Enfermagem na Área de Especialização em Saúde Infantil e Pediatria. Ele comtempla a descrição e análise crítica das aprendizagem realizadas ao longo de diversas experiências de estágio, explicitando os objetivos delineados, as atividades desenvolvidas e os processos de trabalho utilizados, bem como as competências de enfermeiro especialista adquiridas e/ou desenvolvidas. A moldura teórica que norteou o percurso de está...

  13. Raça e comportamento político: participação, ativismo e recursos em Belo Horizonte

    OpenAIRE

    Bueno,Natália S.

    2012-01-01

    A discussão sobre raça na política brasileira é mote entre cientistas sociais de diversas perspectivas: seja pelo seu papel na formação do Estado nacional, nos movimentos sociais ou ainda nas políticas públicas voltadas a grupos raciais. Utilizando a abordagem centrada em recursos, argumenta-se que o pertencimento a um grupo racial não afeta de forma substantiva a propensão a atuar politicamente em Belo Horizonte, ao passo que fatores socioeconômicos, como renda e escolaridade, são nitidament...

  14. Uso de análise espectral e regras de filtragem em operações com contratos futuros de soja no Brasil

    OpenAIRE

    Souza,Waldemar Antônio da Rocha de; Carmo Filho,Manoel Martins do; Martines Filho,João Gomes; Marques,Pedro Valentim

    2013-01-01

    Objetivou-se examinar a hipótese de eficiência semiforte de mercado (HEM) nos contratos futuros de soja do Brasil, negociados na BM&F-Bovespa, aplicando análise espectral e regras de filtragem. Os resultados comparados da teoria indicam conclusões diversas quanto à HEM em mercados futuros de commodities agropecuárias. Entretanto, a eficiência semiforte de mercado parece não se confirmar em preços futuros de soja. Após o teste, rejeitou-se o modelo de passeio aleatório para os preços futuros d...

  15. Resíduos da construção civil em Araguari-MG : do diagnóstico à proposta de um modelo gerencial proativo

    OpenAIRE

    Marques, Rogério Borges

    2007-01-01

    Levantamentos recentes realizados em diversas cidades brasileiras mostram que os RCD Resíduos da Construção e Demolição representam, em alguns casos, mais de 50% da massa dos resíduos sólidos urbanos. Os municípios têm encontrado dificuldades para gerenciar de forma eficaz as grandes quantidades de RCD e solucionar o problema das deposições irregulares que comprometem a qualidade de vida da população e ocasionam sérios problemas sócio-ambientais e sanitários. Este trabalho fe...

  16. Formalização de um sistema de procedimentos para gerenciamento e coordenação de projetos em escritórios de arquitetura

    OpenAIRE

    Bragaglia, Umberto João

    2006-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia Civil. O desenvolvimento do projeto de uma edificação se inicia na organização dos programas e do planejamento, não se resume somente na entrega de um jogo de desenhos e documentos. Inclui todo um conjunto de projetos de arquitetura e engenharia que são elaborados em diversas etapas, seguindo uma seqüência de atividades a serem desenvolvidas simultaneamente, que nem ...

  17. VIII Graduação em Campo: espaço a jovens pesquisadores e incentivo à pesquisa de campo na área de Antropologia Urbana

    OpenAIRE

    2014-01-01

    Em setembro, ocorreu a oitava edição do Seminário Graduação em Campo, evento realizado pelo NAU – Núcleo de Antropologia Urbana da USP. O evento foi criado para que os alunos de graduação dos cursos de Ciências Sociais e Antropologia no Brasil pudessem apresentar seus trabalhos de pesquisa, valorizando assim a pesquisa de campo  nas diversas instituições de ensino do país. Durante o congresso, os alunos puderam assistir a filmes etnográficos e também participar de minicursos ministrados pelos...

  18. Geração de carga sintética para o planejamento de capacidade em ambientes de servidores virtuais

    OpenAIRE

    Estanislau Soares Galindo, Hugo

    2010-01-01

    Atualmente os sistemas de informação estão presentes em todas as atividades do nosso cotidiano, sendo aplicados em diversas áreas, desde saude, comunicação e educação ate finanças, seguranca e entretenimento. Dado o uso disseminado destes sistemas, a indisponibilidade ou desempenho insuficiente dos serviços providos têm sido objetos de atenção particular dos provedores de servicos, infraestruturas, projetistas e da comunidade científica. O planejamento de capacidade destas infr...

  19. Contribuição para o estudo da presença e remoção de compostos emergentes de filtros de UV em ETAR

    OpenAIRE

    Simões, Pedro Miguel da Silva Vieira

    2010-01-01

    Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do Grau de Mestre em Engenharia do Ambiente – Perfil Engenharia Sanitária Grande parte das substâncias produzidas pelas actividades humanas acabam por chegar às estações de tratamento de águas residuais (ETAR). Os compostos constituintes dos filtros de UV, considerados micropoluentes emergentes, são utilizados em diversas aplicações, sobretudo: produtos de higiene pessoal (PHP), pneus...

  20. Neonatal Phosphate Nutrition Alters <em>in em>Vivo> and <em>in em>Vitro> Satellite Cell Activity in Pigs

    Directory of Open Access Journals (Sweden)

    Chad H. Stahl

    2012-05-01

    Full Text Available Satellite cell activity is necessary for postnatal skeletal muscle growth. Severe phosphate (PO4 deficiency can alter satellite cell activity, however the role of neonatal PO4 nutrition on satellite cell biology remains obscure. Twenty-one piglets (1 day of age, 1.8 ± 0.2 kg BW were pair-fed liquid diets that were either PO4 adequate (0.9% total P, supra-adequate (1.2% total P in PO4 requirement or deficient (0.7% total P in PO4 content for 12 days. Body weight was recorded daily and blood samples collected every 6 days. At day 12, pigs were orally dosed with BrdU and 12 h later, satellite cells were isolated. Satellite cells were also cultured <em>in vitroem> for 7 days to determine if PO4 nutrition alters their ability to proceed through their myogenic lineage. Dietary PO4 deficiency resulted in reduced (<em>P> < 0.05 sera PO4 and parathyroid hormone (PTH concentrations, while supra-adequate dietary PO4 improved (<em>P> < 0.05 feed conversion efficiency as compared to the PO4 adequate group. <em>In vivoem> satellite cell proliferation was reduced (<em>P> < 0.05 among the PO4 deficient pigs, and these cells had altered <em>in vitroem> expression of markers of myogenic progression. Further work to better understand early nutritional programming of satellite cells and the potential benefits of emphasizing early PO4 nutrition for future lean growth potential is warranted.

  1. Colisepticemia em Papagaio verdadeiro ("Amazona aestiva" - Relato de Caso

    Directory of Open Access Journals (Sweden)

    Júlio Lopes Sequeira

    2007-05-01

    Full Text Available Colibacilose é o termo comumente empregado para designar as infecções por Escherichia coli nos animais, sendo a colisepticemia um dos quadros causados por esta. Durante muito tempo a E. coli foi considerada um microrganismo não patogênico, porém alguns sorogrupos começaram a ser associado com diversas patologias no homem e nos animais domésticos. O presente artigo relata a ocorrência de colisepticemia em um Papagaio verdadeiro (Amazona aestiva atendido no Laboratório de Ornitopatologia do Hospital Veterinário da Faculdade de Medicina Veterinária e Zootecnia da Universidade Estadual Paulista (FMVZ-UNESP, campus Botucatu-SP, Brasil.

  2. Gêneros introdutórios em livros acadêmicos

    OpenAIRE

    Gomes Bezerra, Benedito

    2006-01-01

    Neste estudo, buscamos esclarecer, à luz da análise de gêneros de linha swalesiana, aspectos da constituição e identificação dos gêneros introdutórios em livros acadêmicos, considerando as diversas formas de sua atualização como artefato textual, sua relação com o suporte e o conjunto de propósitos comunicativos que procuram realizar. Para a análise sócio-retórica dos gêneros introdutórios, utilizamos como referencial os princípios gerais da teoria proposta por Swales (1990, 20...

  3. Constituents from <em>Vigna em>vexillata> and Their Anti-Inflammatory Activity

    Directory of Open Access Journals (Sweden)

    Guo-Feng Chen

    2012-08-01

    Full Text Available The seeds of <em>Vigna em>genus are important food resources and there have already been many reports regarding their bioactivities. In our preliminary bioassay, the chloroform layer of methanol extracts of<em> V. vexillata em>demonstrated significant anti-inflammatory bioactivity. Therefore, the present research is aimed to purify and identify the anti-inflammatory principles of <em>V. vexillataem>. One new sterol (1 and two new isoflavones (2,3 were reported from the natural sources for the first time and their chemical structures were determined by the spectroscopic and mass spectrometric analyses. In addition, 37 known compounds were identified by comparison of their physical and spectroscopic data with those reported in the literature. Among the isolates, daidzein (23, abscisic acid (25, and quercetin (40 displayed the most significant inhibition of superoxide anion generation and elastase release.

  4. Prevalência de micro-organismo e sensibilidade antimicrobiana de infecções hospitalares em unidade de terapia intensiva de hospital público no Brasil

    Directory of Open Access Journals (Sweden)

    Lívia Moreira Barros

    2012-08-01

    Full Text Available As Infecções em Serviços de Saúde (ISS são consideradas problema de saúde pública, causando impacto na morbidade e mortalidade, no tempo de internação, nos custos com procedimentos diagnósticos e terapêuticos, impactando também o paciente, a família e a comunidade. Portanto, esse estudo objetivou-se a identificar prevalência, localização, micro-organismo e sensibilidade antimicrobiana de infecções hospitalares em Unidades de Terapia Intensiva. A população foi constituída por 702 pacientes adultos em tratamento intensivo, de janeiro a dezembro de 2007. A coleta de dados ocorreu a partir de consulta às fichas de busca ativa de infecção hospitalar e preenchimento de formulário. Os dados foram compilados em figuras e tabelas. A infecção hospitalar esteve presente em 202 (28,77% dos pacientes, predominantemente nos meses de dezembro, outubro, setembro e agosto; 132 (64,1% foram caracterizados como infecção respiratória. Os principais microrganismos responsáveis por infecções Pseudomonas aeruginosa 33,8% (51, S. aureus 25,2% (38, Acinetobacter sp. 12,6% (19, Enterobacter agglomerans 8,6% (13, Pseudomonas sp. 7,3% (11, E. coli 4,6% (7, Klebsiella pneumoniae 4,6% (7 e Candida sp. 3,3% (5. Os beta-lactâmicos apresentaram alta sensibilidade nos antibiogramas. As dificuldades de prevenção de infecções hospitalares se potencializam no ambiente de terapia intensiva, pelo comprometimento imunológico do paciente, variedade de microrganismos multiresistentes e uso de antimicrobianos de amplo espectro.

  5. Sintomatologia da murcha de Ceratocystis fimbriata em eucalipto Symptomatology of Ceratocystis wilt in eucalyptus

    Directory of Open Access Journals (Sweden)

    Francisco Alves Ferreira

    2006-04-01

    Full Text Available Descreveram-se o histórico da murcha de Ceratocystis fimbriata em eucalipto no Brasil e em outros países e a sintomatologia da doença, em plantações clonais com 4 meses a 5 anos de idade, em brotações de tocos, em estacas em enraizamento e em mudas clonais em viveiro, de quatro estados brasileiros. O patógeno evoluía-se da extremidade da raiz, atingindo o colo e tronco acima via parênquima medular, de onde, em diversas alturas, surgiam estrias escuras, que progrediam, via parênquima radial, matando uma porção de câmbio vascular, de floema e de feloderme. Dessa progressão sistêmica do patógeno, ascendente e radialmente, resultava uma lesão longitudinal externamente no tronco, contínua ou descontínua, marrom-avermelhada, coriácea, que passava a sulcada e, posteriormente, a cancro longitudinal, com seus calos longilíneos nas duas laterais. Por esse contexto sintomatológico, pode-se considerar essa enfermidade como um modelo de doença sistêmica em essência florestal, pelo menos na subárea da patologia florestal brasileira. Em brotações novas, em estacas em enraizamento e em mudas clonais as lesões eram longitudinais, contínuas ou descontínuas, negras a arroxeadas. A inativação de xilema em raízes, colo e em diferentes alturas do tronco, ou galho, dava-se pelo adensamento das estrias radiais escuras no lenho.The symptomatology of Ceratocystis fimbriata wilt in eucalyptus was described based on observations of 4-month to 5-year-old clonal plantations, in stump sprouts, rooting cuttings, and rooted seedlings in nurseries. The disease was characterized as a model of systemic disease in woody plants, which starts in roots and progresses upward to the collar and trunk through the medullar parenchyma, where dark stripes irradiate and lead a portion of the vascular cambium, phloem and phelloderm to death. As the pathogen spread upward and radially, a longitudinal reddish-brown, continuous or non-continuous corky lesion

  6. O Voluntariado em questão: a subjetividade permitida

    Directory of Open Access Journals (Sweden)

    Adriana Cristina Ferreira Caldana

    Full Text Available Em diversos países, particularmente no Brasil, observa-se um estímulo ao voluntariado como via de democratização do Estado, com o aumento da participação de todos os atores nos graves problemas sociais. considerando as diversas expressões de atuação voluntária no mundo do trabalho, o objetivo do presente artigo é discutir o exercício dessas atividades e de suas lógicas de sustentação a partir dos principais eixos de identificação com a atividade voluntária. Estudos de casos foram realizados em duas empresas com fins lucrativos e em duas organizações não governamentais (ONGs da cidade de Ribeirão Preto (São Paulo-Brasil. Os dados indicam que o voluntariado, indiretamente, gera valor para o capital e que se deve investir na autonomia das ONGs e na superação de modelos assistencialistas, com ações particularizadas, fortalecendo os empreendimentos coletivos da economia solidária e os movimentos sociais de base. considera-se que o voluntariado seja um modo permitido de construção da subjetividade dentro da lógica do capital.

  7. Osseointegração de implantes em defeitos circunferenciais utilizando proteínas angiogênicas purificadas do látex, osso autógeno e regeneração óssea guiada. Estudo comparativo em mandíbulas de cães

    OpenAIRE

    Maya Fernanda Manfrin Arnez

    2009-01-01

    Diversas pesquisas têm direcionado esforços na busca de biomateriais que fossem capazes de melhorar a osseointegração e a estabilidade de implantes dentais em regiões anatômicas desfavoráveis. Entretanto, muitas das opções de terapias regenerativas, largamente empregadas em cirurgias orais reconstrutivas, estão associadas à algumas desvantagens clínicas como um grande período de recuperação, alto risco de morbidade, risco de parestesia nos pacientes, além de apresentarem alto custo. Biomateri...

  8. Estudo comparativo entre as técnicas de diluição em caldo e diluição em ágar, nos antibiogramas para Candida: a comparative study

    OpenAIRE

    Alves,Sydney Hartz; Cury,Ariete Emily

    1992-01-01

    Os autores compararam o desempenho das técnicas de diluição em caldo e diluição em ágar, mediante a determinação da CIM (concentração inibitória mínima) e da CFM (concentração fungicida mínima) de antifúngicos poliênicos e imidazólicos, frente a diversas espécies de Candida. A concordância entre as técnicas foi variável em função do antifúngico utilizado. Os melhores percentuais de concordância ocorreram quando se realizou o teste com poliênicos. Os autores discutem aspectos que envolvem a pr...

  9. Gerenciamento de resíduos sólidos urbanos: um estudo de caso em uma escola estadual de ensino médio em Pombal-PB

    OpenAIRE

    Crispim, D. L.; UFCG; Fernandes, J. D.; UFRN/UAB; Andrade, S. O.; UFCG/IFRN; Sousa, E. P.; UFCG/IFRN; Sales, J. C. F.; UFCG

    2016-01-01

    Diversas cidades brasileiras apresentam dificuldades relacionadas à gestão de resíduos sólidos, em especial na coleta, transporte, tratamento e no destino final de resíduos. A deficiência na gestão dos resíduos sólidos permite que práticas inadequadas de disposição final sejam comuns, como a disposição em lugar impróprio. O estudo objetivou verificar o nível de conhecimento dos discentes da Escola E.M.M.V.F. em Pombal, PB, sobre o gerenciamento dos resíduos sólidos urbanos. A pesquisa foi rea...

  10. Estudo paramétrico da injeção de vapor em reservatórios delgados

    OpenAIRE

    Gurgel, Antônio Robson

    2009-01-01

    A cada ano tem ocorrido cada vez mais a exploração de reservas de óleo pesado em diversas regiões do mundo. Isto tem sido feito porque é cada vez mais rara a existência de jazidas petrolíferas de óleos leves. Este fato tem impulsionado inúmeras pesquisas em direção a tornar viável, do ponto de vista operacional e econômico, a exploração de tais reservas de óleo. No Brasil, em especial na região Nordeste, existe uma grande quantidade de reservatórios de óleos pesados, onde a recuperação pel...

  11. O humor em contos de Moacyr Scliar: um representante da ficção contemporânea

    Directory of Open Access Journals (Sweden)

    Angela Maria Pelizer de Arruda

    2012-10-01

    Full Text Available Moacyr Scliar é contista, cronista e romancista em consonância com o seu tempo, utiliza o o humor em várias de suas obras e em diversas situações. Para a seleção do corpus ficcional, o conto será o gênero privilegiado, já que este é considerado o gênero da contemporaneidade por sua capacidade de síntese e por ser capaz de retratar a realidade do homem moderno, já que se utiliza de temas simples, dos fatos miúdos, próprios do cotidiano do sujeito comum. Com isso, este artigo pretende traçar um paralelo entre a arte ficcional de Moacyr Scliar e suas tendências pós modernas e o conceito de humor.

  12. Diversidade e dinâmica populacional de ácaros em pomar cítrico

    OpenAIRE

    Silva,Marcos Zatti da; Sato,Mário Eidi; Oliveira,Carlos Amadeu Leite de

    2012-01-01

    As plantas de citros hospedam diversas espécies de ácaros que causam sérios prejuízos à produção e qualidade de frutos no Brasil. Esta pesquisa teve por objetivo estudar a diversidade de ácaros e a dinâmica populacional das principais espécies de ácaros em pomar de citros, Citrus sinensis (L.) Osbeck, localizado no município de Descalvado, Estado de São Paulo. Foram realizadas 14 avaliações em um período de 12 meses, de fevereiro de 2007 a fevereiro de 2008. Em cada avaliação, foram coletadas...

  13. Relatório de estágio : mestrado em análises clínicas

    OpenAIRE

    Marques, Daniela Monteiro

    2016-01-01

    Relatório de estágio do mestrado em Análises Clínicas, apresentado à Faculdade de Farmácia da Universidade de Coimbra Neste relatório o objetivo principal foi o de descrever as atividades que são executadas no LAC da Faculdade de Farmácia de Coimbra, tendo em especial atenção a área da hematologia pois esta foi a que escolhi para descrever em mais pormenor. No LAC são prestados serviços de análises clínicas de diversas valências, como a bioquímica, microbiologia, imunologia, en...

  14. Estudos transcriptômicos no contexto da conectividade perturbada em esquizofrenia

    Directory of Open Access Journals (Sweden)

    Andrea Schmitt

    2013-01-01

    Full Text Available Esquizofrenia é uma severa doença neurobiológica com fatores genéticos e ambientais desempenhando um papel na fisiopatologia. Diversas regiões cerebrais têm sido implicadas no processo da doença e estão conectadas em complexos circuitos neuronais. Nos níveis molecular e celular, a conectividade afetada entre essas regiões, envolvendo mielinização disfuncional dos axônios neuronais, bem como as alterações no nível sináptico e metabolismo energético levando a distúrbios na plasticidade sináptica, são os maiores achados em estudos post-mortem. Estudos de microarranjos investigando a expressão gênica contribuíram para os achados de alterações em vias complexas em regiões cerebrais relevantes na esquizofrenia. Além disso, estudos utilizando microdissecção e captura a laser permitiram a investigação da expressão gênica em grupos específicos de neurônios. Entretanto, deve ser mantido em mente que em estudos post-mortem, confusos efeitos de medicação, qualidade de RNAm, bem como capacidade de mecanismos regenerativos neuroplásticos do cérebro em indivíduos com história de vida de esquizofrenia, podem influenciar o complexo padrão de alterações no nível molecular. Apesar dessas limitações, estudos transcriptômicos livres de hipóteses em tecido cerebral de pacientes esquizofrênicos oferecem uma possibilidade única para aprender mais sobre os mecanismos subjacentes, levando a novas ópticas da fisiopatologia da doença.

  15. Dermatoses em renais cronicos em terapia dialitica

    Directory of Open Access Journals (Sweden)

    Luis Alberto Batista Peres

    2014-03-01

    Full Text Available Objetivo: As desordens cutâneas e das mucosas são comuns em pacientes em hemodiálise a longo prazo. A diálise prolonga a expectativa de vida, dando tempo para a manifestação destas anormalidades. Os objetivos deste estudo foram avaliar a prevalência de problemas dermatológicos em pacientes com doença renal crônica (DRC em hemodiálise. Métodos: Cento e quarenta e cinco pacientes com doença renal crônica em hemodiálise foram estudados. Todos os pacientes foram completamente analisados para as alterações cutâneas, de cabelos, mucosas e unhas por um único examinador e foram coletados dados de exames laboratoriais. Os dados foram armazenados em um banco de dados do Microsolft Excel e analisados por estatística descritiva. As variáveis contínuas foram comparadas pelo teste t de Student e as variáveis categóricas utilizando o teste do qui-quadrado ou o teste Exato de Fischer, conforme adequado. Resultados: O estudo incluiu 145 pacientes, com idade média de 53,6 ± 14,7 anos, predominantemente do sexo masculino (64,1% e caucasianos (90,0%. O tempo médio de diálise foi de 43,3 ± 42,3 meses. As principais doenças subjacentes foram: hipertensão arterial em 33,8%, diabetes mellitus em 29,6% e glomerulonefrite crônica em 13,1%. As principais manifestações dermatológicas observadas foram: xerose em 109 (75,2%, equimose em 87 (60,0%, prurido em 78 (53,8% e lentigo em 33 (22,8% pacientes. Conclusão: O nosso estudo mostrou a presença de mais do que uma dermatose por paciente. As alterações cutâneas são frequentes em pacientes em diálise. Mais estudos são necessários para melhor caracterização e manejo destas dermatoses.

  16. Os trabalhadores bolivianos em São Paulo: uma abordagem jurídica

    OpenAIRE

    Gabrielle Louise Soares Timóteo

    2011-01-01

    No atual cenário de globalização são verificadas diversas práticas de exploração laboral. O trabalho escravo é a forma de exploração laboral mais intensa que pode existir nos dias atuais. O tráfico de pessoas é uma prática criminosa crescente em nossa sociedade. No Brasil, em São Paulo, existem casos de trabalhadores imigrantes bolivianos vítimas de tráfico de pessoas e trabalho escravo. Esta pesquisa busca discutir os conceitos de trabalho decente, trabalho escravo, tráfico de pessoas, tráfi...

  17. Dermatose responsiva à biotina em cão Dermatosis responsive to Biotin in a dog

    OpenAIRE

    Sandra Prudente Nogueira; Márcio Antonio Brunetto; Juliana Toloi Jeremias; Márcia de Oliveira Sampaio Gomes; Eliana Teshima; Aulus Cavalieri Carciofi

    2010-01-01

    Os transtornos da pele e dos pelos são parte importante na prática clínica de pequenos animais. Numerosos fatores nutricionais afetam a homeostase, a qualidade e o aspecto da pelagem. As vitaminas do complexo B incluem compostos hidrossolúveis necessários como coenzimas em diversas funções celulares envolvidas no metabolismo energético e na síntese tecidual. A biotina, em especial, é necessária nas reações de carboxilação, participando da síntese de ácidos graxos, aminoácidos e purinas pelo t...

  18. Estratégias de polidez na interação em aulas chat

    OpenAIRE

    CUNHA, Eva Carolina Da

    2009-01-01

    Os estudos sobre a polidez lingüística, apesar de amplos em diversas situações de interação verbal, parecem escassos quando na área da interação virtual. Este trabalho visa compreender como se dão as relações interacionais nesse meio, como as pessoas usam suas falas com a finalidade de evitar situações de ofensas e de buscar uma interação fluida e harmônica, já que as relações sociais estabelecidas em ambientes virtuais parecem tão confusas e propensas à má compreensão ou a ...

  19. Avaliação do interesse na carreira acadêmica dos médicos em especialização em Anestesiologia

    Directory of Open Access Journals (Sweden)

    Gustavo Henrique Meurer

    2010-12-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: Tem-se observado certa tendência à perda pelo interesse acadêmico nas diversas áreas da Medicina, inclusive Anestesiologia. O objetivo deste estudo foi pesquisar o interesse de médicos em especialização (ME em Anestesiologia em seguir uma carreira acadêmica, bem como identificar os fatores determinantes dessa escolha. MÉTODO: O estudo foi prospectivo e transversal. Selecionou-se uma amostra probabilística simples de médicos em especialização em Anestesiologia. Um questionário foi enviado a cada participante. As respostas foram submetidas a análises uni e multivariadas para determinar a frequência de predisposição acadêmica na amostra e determinação dos fatores preditivos independentes de predisposição acadêmica. RESULTADOS: Foram analisados 155 questionários (taxa de resposta = 38,7%. Sessenta e nove participantes (44,5% manifestaram interesse em seguir uma carreira acadêmica. A análise multivariada identificou como fatores independentes de previsão da predisposição acadêmica: estar cursando o primeiro ano do curso de especialização (razão de chances [RC] = 2,52; intervalo de 95% de confiança [IC 95%] = 1,19 - 5,38, ter apresentado trabalho científico em evento médico (RC = 3,78; IC 95% = 1,84 - 7,78 e pertencer à região sudeste (RC = 2,66; IC 95% = 1,31 - 5,39. CONCLUSÕES: Uma parcela significativa dos ME brasileiros demonstrou interesse em seguir carreira acadêmica após o término do curso de especialização. Em comparação com a probabilidade de não manifestar predisposição acadêmica, os médicos do primeiro ano do curso de especialização apresentam chances 2,5 vezes maiores de se manifestarem interessados em uma carreira acadêmica; apresentação de trabalho científico em eventos médicos aumentou essa chance em 3,78 vezes; pertencer a CETs da região sudeste aumentou em 2,66 vezes a chance manifestar interesse acadêmico.

  20. Evidências da circulação de conhecimento filosófico-natural sobre o Brasil em um manuscrito de 1763 de António Nunes Ribeiro Sanches

    Directory of Open Access Journals (Sweden)

    Gisele C. Conceição

    Full Text Available Resumo O médico português António Nunes Ribeiro Sanches foi autor de vários trabalhos sobre educação e ciência em Portugal e em seu Império. Muitos dos textos que escreveu circularam em sua extensa rede de contatos e foram importantes para a formação e consolidação de diversas políticas públicas, principalmente a partir do governo do marquês de Pombal. Neste artigo, exponho algumas das ideias de Ribeiro Sanches sobre o reconhecimento dos recursos naturais brasileiros e sua exploração para o comércio e a medicina em um manuscrito, até então inédito, escrito em 1763.

  1. Determinação da produção de metalo-b-lactamases em amostras de Pseudomonas aeruginosa isoladas em João Pessoa, Paraíba

    OpenAIRE

    Santos Filho Lauro; Santos Isabele Beserra; Assis Alexandro Mangueira Lima de; Xavier Danilo Elias

    2002-01-01

    Bactérias produtoras de metalo-beta-lactamases (MBLs) são em grande parte resistentes aos betalactâmicos de largo espectro, incluindo oximino-aminotiazol cefalosporinas e também aos carbapenens. O objetivo deste trabalho foi detectar cepas de Pseudomonas aeruginosa resistentes ao imipenem e à ceftazidima, assim como identificar aquelas produtoras de MBLs. Foram estudadas 198 linhagens não-repetitivas isoladas de diversas amostras clínicas, hospitalares e comunitárias, identificadas bioquimica...

  2. Determinação da produção de metalo-beta-lactamases em amostras de Pseudomonas aeruginosa isoladas em João Pessoa, Paraíba

    OpenAIRE

    Santos Filho,Lauro; Santos,Isabele Beserra; Assis,Alexandro Mangueira Lima de; Xavier,Danilo Elias

    2002-01-01

    Bactérias produtoras de metalo-beta-lactamases (MBLs) são em grande parte resistentes aos betalactâmicos de largo espectro, incluindo oximino-aminotiazol cefalosporinas e também aos carbapenens. O objetivo deste trabalho foi detectar cepas de Pseudomonas aeruginosa resistentes ao imipenem e à ceftazidima, assim como identificar aquelas produtoras de MBLs. Foram estudadas 198 linhagens não-repetitivas isoladas de diversas amostras clínicas, hospitalares e comunitárias, identificadas bioquimica...

  3. Estudo de manifestações patológicas em revestimento de fachada em Brasília - sistematização da incidência de casos

    OpenAIRE

    Antunes, Giselle Reis

    2011-01-01

    Atualmente a indústria da construção civil, nos cenários nacional e internacional, tem sido marcada pelo uso de novos materiais e desenvolvimento de tecnologias construtivas pouco abordadas cientificamente. No que se refere ao sistema de revestimento de fachada, apesar desta evolução tecnológica em curso, é crescente a incidência de manifestações patológicas com origens diversas. O presente trabalho busca colaborar para o estudo de manifestações patológicas, através de uma metodologia sistemá...

  4. Algoritmos Imunoinspirados aplicados em segurança computacional: utilização de algoritmos inspirados no sistema imune para detecção de intrusos em redes de computadores

    OpenAIRE

    Joaquim Quinteiro Uchoa

    2009-01-01

    A evolução da Internet e a contínua interconexão de computadores e outros dispositivos digitais têm criado uma série de oportunidades nas mais diversas áreas de atuação humana. Entretanto, esse processo trouxe consigo uma série de problemas, entre eles o crescente número de sistemas computacionais invadidos por intrusos. O trabalho aqui apresentado aborda esse problema utilizando metodologias e algoritmos inspirados em conceitos e processos do sistema imune inato e adaptativ...

  5. KAWAUCHE, Thomaz Massadi. Religião e Política em Rousseau. São Paulo: Humanitas, 2013.

    Directory of Open Access Journals (Sweden)

    Lauro Iane de Morais

    2015-07-01

    Full Text Available Lançado em 2013 pela editora Humanitas, o livro analisa a relação entre a religião civil e o Estado à luz do contrato social nas obras de Rousseau. Para essa tarefa, o autor contou com uma extensa referência bibliográfica que inclui, além das mais destacadas obras de Rousseau, cartas suas endereçadas a diversas personalidades, tais como Voltaire e d'Alembert, que estavam em diálogo direto com o genebrino. E quem melhor para analisar tão minuciosamente o tema que Thomaz Kawauche, graduado pela Universidade de São Paulo em Engenharia Mecânica 1998 e Filosofia 2005. Defendeu na mesma instituição, o mestrado em 2007, doutorado em 2012 e o pós-doutorado em 2013, todos na área de Ética e Filosofia Política, tendo como objeto de pesquisa a obra de J.-J. Rousseau. oration:none;text-line-through:none'>O livro possui quatro capítulos repletos de subdivisões que organizam muito bem seu desenvolvimento conceitual, sendo os dois primeiros dedicados à tolerância civil e à religiosa, o terceiro aos limites da tolerância e o quarto à noção de moralidade em Locke.

  6. Vibrio spp. and Salmonella spp., presence and susceptibility in crabs Ucides cordatus Vibrio spp. e Salmonella spp. em caranguejos, Ucides cordatus

    Directory of Open Access Journals (Sweden)

    Regine H.S.F. Vieira

    2004-08-01

    Full Text Available The presence of Vibrio spp. and Salmonella spp. in crabs marketed at the Bezerra de Menezes Ave., Fortaleza, State of Ceará, Brazil, was assessed between February and May, 2003. The number of individuals sampled in each one of the fifteen weekly samplings ranged between four and eight. Seven strains of Salmonella, from four different samplings, were identified, being five of them identified as serotype S. Senftenberg and two as S. Poona. All strains of Salmonella were sensitive to the tested anti-microbial drugs, with the exception of tetracycline and nalidixic acid, for which an intermediary sensibility was found. The MPN's for Vibrio ranged between 110/g and 110,000/g. Of the forty five Vibrio strains isolated from the crab samples, only 10 were identified up to the species level: two V. alginolyticus and eight V. parahaemolyticus. Bacteria belonging to the Enterobacteriaceae and Pseudomonaceae families were also identified, namely Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Pantoea agglomerans and Pseudomonas aeruginosa. The proper cooking of the animals is recommended in order to avoid problems for the consumers of this crustacean.Foram pesquisadas a presença de Vibrio spp. e de Salmonella spp. em caranguejos comercializados na Av. Bezerra de Menezes, Fortaleza, Ceará, Brasil, no período entre fevereiro e maio de 2003. O número de indivíduos em cada, das quinze coletas realizadas, semanalmente, variava entre quatro e oito dependendo do tamanho dos animais, totalizando um número de 90 (noventa animais examinados. Foram identificadas sete cepas de Salmonella spp. provenientes de quatro coletas: cinco foram identificadas como sorovar S. Senftenberg e duas como S. Poona. Todas as cepas de Salmonella, isoladas das amostras de caranguejos, apresentaram sensibilidade aos antimicrobianos testados, com exceção de tetraciclina e ácido nalidíxico para os quais elas apresentaram uma sensibilidade intermediária. Os NMPs

  7. Indicadores de sustentabilidade em indústrias de vestuário no APL de Maringá/Cianorte - PR

    OpenAIRE

    Barcelos, Silvia Mara Bortoloto Damasceno

    2013-01-01

    De origem latina “sustentare” significa suster, sustentar, suportar, conservar em bom estado, manter e resistir, logo, a expressão sustentável quer dizer capacidade de ser mantido ou suportado. A sustentabilidade encontra-se fundamentada no Triple Botton Line, denominado também de pilares ou dimensões. Nesse contexto, suas diversas áreas, vem ganhando elevada importância, devido a problemas ambientais, econômicos e socais, tornando-se uma ferramenta de diferencial entre as organizações, at...

  8. Thémis desnudada: a questão da justiça em Hans Kelsen

    OpenAIRE

    Eduardo de Oliveira Valory

    2013-01-01

    Embora Hans Kelsen tenha desenvolvido suas ideias sobre a justiça em diversos artigos e capítulos de livros, o jusfilósofo nunca edificou uma obra mais profunda, monográfica ou sistemática sobre a questão do justo. Suas considerações, o mais das vezes proferidas incidentalmente quando da análise e crítica das teorias do direito natural, se encontram, a bem dizer, dispersas por diversas produções. A leitura integral e conjunta de seus estudos, entretanto, permite a identificação da mesma e coe...

  9. Mandatos mundiais em subsidiárias no Brasil: uma análise sob a perspectiva da teoria neoinstitucional

    OpenAIRE

    Juliana Bittar de Souza

    2011-01-01

    O objetivo deste trabalho foi investigar a relação entre o ambiente institucional brasileiro e a conquista de mandatos mundiais pelas subsidiárias estrangeiras. Como parte de um novo modelo de empresa multinacional que precisa combinar a organização de uma rede integrada que proporcione flexibilidade, facilite o aprendizado, a transferência de conhecimento com a inovação entre as diversas unidades (BARTLETT, GHOSHAL, 1998), a subsidiária se destaca como unidade de análise na literatura em est...

  10. Insegurança e criminalidade urbana: um estudo sobre estratégias midiáticas em telejornais

    OpenAIRE

    Mattos, Alexandre Pereira de

    2010-01-01

    Esta pesquisa tem como foco a insegurança ontológica. Na psicologia, a insegurança é trabalhada como substrato da experiência de pessoalidade a partir dos processos de socialização que encontramos nas teorizações de Winnicott e, dentro de uma perspectiva da psicologia social, nas de Mead. Na contemporaneidade a insegurança está associada aos riscos que atravessam nossas práticas cotidianas dentre elas a criminalidade violenta. Em meio às diversas possibilidades de contato co...

  11. A FISIOTERAPIA NA PREVENÇÃO E DIMINUIÇÃO DA INCIDÊNCIA DE QUEDAS EM IDOSOS

    OpenAIRE

    Gabriela Fernandez; Claudia Russi

    2017-01-01

    O processo de envelhecimento é um acontecimento fisiológico e traz diversas alterações anatômicas na postura, no sistema articular e osteomuscular, alterações no sistema nervoso e cardiorrespiratório; assim como no sistema imunológico, digestivo, endócrino e metabólico. Estas alterações tornam o idoso mais frágil e propenso a sofrer quedas. As quedas são ocorrências relativamente comuns e constituem causa de morbidade e mortalidade em indivíduos com mais de 65 anos. Portanto, foi realizada um...

  12. Gestão do disponível: um estudo em micro, pequenas e médias empresas

    OpenAIRE

    Barbosa, Daiane dos Santos; Quintana, Alexandre Costa

    2013-01-01

    Em mercados cada vez mais exigentes, as empresas buscam a melhor maneira de administrar suas finanças, de forma a garantir a permanência no mercado e a maximização do resultado. Neste sentido, a administração financeira vem a contribuir com este propósito, por meio de mecanismos que proporcionam a adequada gestão dos recursos. A gestão das disponibilidades é um grande desafio para as empresas, sendo necessário que o gestor tenha conhecimento sobre as diversas técnicas de administração de caix...

  13. Aplicação do Sistema Global de Manufatura exuta em uma área administrativa

    OpenAIRE

    Alex Ferrante

    2011-01-01

    Com o mercado automobilístico cada vez mais concorrido, tanto no Brasil como no mundo, a necessidade de se ter qualidade de produto e serviços competitivos vem estimulando diversas empresas a investirem em sistemas de manufatura enxuta como forma de favorecer a melhoria dos níveis de qualidade e produtividade, com reflexos diretos sobre a redução dos custos. Dentro desse contexto, o presente trabalho tem como objetivo verificar como os princípios do Sistema Global de Manufatura (SGM) podem se...

  14. SEXUALIDADE MÍTICA: AS MÚLTIPLAS FACES DA FEMINILIDADE EM VÊNUS E MARIA

    OpenAIRE

    Ismael Gonçalves Alves; Pérola de Paula Sanfelice

    2011-01-01

    Este trabalho busca inventariar algumas considerações acerca dos ideais de feminilidade difundidos em dois momentos históricos distintos, a Antiguidade clássica e a Contemporaneidade. Ao optarmos por um estudo comparativo de épocas diversas, não buscamos estabelecer uma linearidade, e sim conhecer múltiplas experiências históricas ao longo do tempo, atravessadas pelas relações que cada sociedade estabelece consigo mesma e com o seu passado. Através da análise das representações de Vênus e Mar...

  15. Ambientes virtuais de aprendizagem como ferramentas de apoio em cursos presenciais e a distância.

    OpenAIRE

    Brito, Lélis Maia de; Giuberti Júnior, José Renato; Gomes, Silvane Guimarães Silva; Mota, João Batista

    2013-01-01

    As tecnologias vêm sendo incorporadas na educação presencial e a distância com o objetivo de enriquecer o processo de ensino-aprendizagem. Uma dessas inovações compreende-se nos ambientes virtuais de aprendizagem (AVAs), que são espaços virtuais nos quais os usuários, os educadores e os educandos podem interagir por meio de diversas ferramentas. A ideia é a de que esses ambientes criem novas possibilidades de aprendizagem ao aluno em face às mudanças tecnológicas que vêm ocorrendo na sociedad...

  16. Estudo de diversas variedades de copas sobre diferentes porta-enxertos da mangueira (Mangifera indica L. Study of several varieties of tree crowns on different rootstocks of mango (Mangifera indica L.

    Directory of Open Access Journals (Sweden)

    S. Simão

    1994-12-01

    Full Text Available O objetivo deste trabalho foi verificar a interferência copa x porta-enxerto no desenvolvimento da mangueira. As alturas e os diâmetros das copas foram tomadas uma vez em 1981, quando as 210 plantas atingiram 15 anos de idade. As variedades de copas estudadas foram as seguintes: Extrema, Oliveira Neto, Carlota, Imperial, Pahiri e Bourbon. Os porta-enxertos utilizados foram Espada, Extrema, Olivara Neto, Carlota, Coco, Pahiri e Bourbon. Os dados foram analisados e a comparação das médias foi feita através do teste de Tukey em nível de 5% de probabilidade. O estudo mostrou que as variedades de copa, Olivara Neto, Extrema e Bourbon atingiram uma altura e um diâmetro maior que as demais variedades.The aim of this paper was to investigate the influence of the combination tree crown + rootstock on mango tree performance. The trees were selected according to height and crown diameter, measured once in 1981, using 210 trees, 15 years old at the beginning of the experiment. The Extrema, Espada, Oliveira Neto, Carlota, Bourbon, Coco and Pahiri varieties were used as rootstock and Extrema, Pahiri, Imperial, Oliveira Neto, Cariota e Bourbon were utilized as scions for tree crown development. Data were analysed by classical statistics, comparing means through the Tukey test, at a probability level of 5%. The crowns Oliveira Neto, Extrema e Bourbon showed the highest heights and diametres.

  17. Biópsia per-oral do intestino delgado em 150 casos de diferentes enteroparasitoses

    Directory of Open Access Journals (Sweden)

    Norton de Figueiredo

    1967-04-01

    Full Text Available Os autores fazem uma apresentação sumária de alguns tipos de instrumentos utilizados na realização da biópsia duodeno-jejunal através intubação oral, ao mesmo tempo que comentam a importância do método no estudo da patologia intestinal. Em seguida apresentam os resultados de sua experiência empregando 6 diferentes tipos de sondas e mostram os resultados histopatológicos observados em 150 casos de diversas parasitoses intestinais, submetidos a estudo. Entre os resultados chamam a atenção para a presença freqüente da Giardia lamblia, detectada em 16 dos 50 (32% casos desta parasitose, contra 3 dos 27 (11,1% de esquistossomose e apenas 2 dos 108 (1,8% de estrongiloidíase. Relatam também o achado de numerosas leishmânias no córion da mucosa jejunal de um paciente parasitado pela Giardia lamblia, que era portador de Calazar. Apreciam, ainda, o significado de outras alterações, consideradas inespecíficas, sobre as quais aguardam estudos mais completos, para chegar a conclusões mais válidas. Entre estas, merece destaque o achado de atrofia das vilosidades intestinais em 27 pacientes infectados pelo Strongyloides stercoialis tendo que em 5 dêles havia parasitismo exclusivo pelo referido nematóide.

  18. Experiências de abortos provocados em clínicas privadas no Nordeste brasileiro

    Directory of Open Access Journals (Sweden)

    Paloma Silveira

    2016-01-01

    Full Text Available Resumo Baseado numa investigação qualitativa desenvolvida em 2012, o artigo analisa experiências de abortos provocados de pessoas de estratos sociais médios realizados em clínicas privadas. Foram narradas 34 histórias de gravidezes interrompidas em clínicas por 19 mulheres e cinco homens, residentes em duas capitais do Nordeste brasileiro. Uma análise temática revela que existem diferentes tipos de clínicas e de atendimento prestados pelos médicos. O artigo mostra que a realização de um aborto em uma clínica privada não é garantia de um atendimento humanizado e seguro. As narrativas fornecem descrições de diversas situações e práticas, desde aquelas com algumas falhas, como a falta de informações sobre os medicamentos, até outras com abusos graves, como procedimentos realizados sem anestesia. Assim, conclui-se que a ilegalidade da prática do aborto, no Brasil, permite que as clínicas funcionem sem qualquer tipo de regulação do Estado, não impedindo que as mulheres realizem abortos, mas as expondo a situações de total vulnerabilidade e de violação dos direitos humanos.

  19. Experiências de abortos provocados em clínicas privadas no Nordeste brasileiro

    Directory of Open Access Journals (Sweden)

    Paloma Silveira

    Full Text Available Resumo Baseado numa investigação qualitativa desenvolvida em 2012, o artigo analisa experiências de abortos provocados de pessoas de estratos sociais médios realizados em clínicas privadas. Foram narradas 34 histórias de gravidezes interrompidas em clínicas por 19 mulheres e cinco homens, residentes em duas capitais do Nordeste brasileiro. Uma análise temática revela que existem diferentes tipos de clínicas e de atendimento prestados pelos médicos. O artigo mostra que a realização de um aborto em uma clínica privada não é garantia de um atendimento humanizado e seguro. As narrativas fornecem descrições de diversas situações e práticas, desde aquelas com algumas falhas, como a falta de informações sobre os medicamentos, até outras com abusos graves, como procedimentos realizados sem anestesia. Assim, conclui-se que a ilegalidade da prática do aborto, no Brasil, permite que as clínicas funcionem sem qualquer tipo de regulação do Estado, não impedindo que as mulheres realizem abortos, mas as expondo a situações de total vulnerabilidade e de violação dos direitos humanos.

  20. Anticorpos catalíticos e suas aplicações em biotecnologia - doi: 10.5102/ucs.v10i2.1583

    Directory of Open Access Journals (Sweden)

    Luciana Martins Macedo

    2012-12-01

    Full Text Available Características peculiares como a alta especificidade dos anticorpos e o eficiente poder catalítico das enzimas, têm despertado um grande interesse em grupos de pesquisa científica no que diz respeito às possíveis reações obtidas pela junção dessas duas moléculas. O objetivo deste trabalho foi realizar uma revisão bibliográfica sobre a constituição e obtenção dos anticorpos catalíticos e suas potenciais aplicações em biotecnologia. A tecnologia de anticorpos catalíticos já demonstrou a capacidade dessas moléculas em mediar diversas reações e vem abrindo relevantes possibilidades de aplicações em áreas industriais, agrícolas, e biotecnológicas. Aliada à prática médica os anticorpos catalíticos são grande promessa para fins diagnósticos e terapêuticos desde a destruição de células cancerígenas e microrganismos, até a elaboração de vacinas catalíticas para as mais diversas finalidades como a desintoxicação definitiva de pacientes com dependência química.

  1. Riqueza e densidade de aves que nidificam em cavidades em plantações abandonadas de eucalipto

    Directory of Open Access Journals (Sweden)

    Hugo de Siqueira Pereira

    2015-05-01

    Full Text Available Monoculturas de árvores, como as de espécies de eucalipto, têm substituído florestas naturais, impactando a fauna pela perda de habitat e recursos. Diversas espécies de aves necessitam de ocos em árvores para nidificar. A falta de recursos como cavidades formadas pela degradação da árvore ou locais apropriados para serem criadas cavidades leva a diminuição na abundância das populações destas espécies. Este trabalho tem o objetivo de comparar a riqueza e a densidade de aves que nidificam em ocos em áreas de plantação de eucalipto abandonadas com áreas de vegetação nativa em duas unidades de conservação em Minas Gerais. Em cada tipo de vegetação de cada reserva foram estabelecidos 25 pontos, nas quais foi utilizado o método de contagem por remoção para o levantamento das espécies de aves. A riqueza de espécies de aves de cada área foi estimada por Jackniffe e a densidade foi estimada através do método de máxima verossimilhança; modelos candidatos que poderiam influenciar a abundância das aves foram avaliados utilizando-se o Critério de Informação Akaike (AIC. Os resultados mostraram que a riqueza de espécies foi maior nas áreas de florestas nativas e que a densidade foi significantemente mais alta nas áreas de florestas nativas, e que o tipo de vegetação e a hora do dia influenciam na abundância e detecção das aves. Esses resultados indicam que as plantações de eucaliptos influenciam negativamente a comunidade de aves que nidificam em ocos, provavelmente por causa da falta de cavidades ou de sub-bosque. Plantações de eucaliptos normalmente não substituem as florestas nativas e seu uso deve ser controlado em áreas de conservação.

  2. EM International. Volume 1

    Energy Technology Data Exchange (ETDEWEB)

    1993-07-01

    It is the intent of EM International to describe the Office of Environmental Restoration and Waste Management`s (EM`s) various roles and responsibilities within the international community. Cooperative agreements and programs, descriptions of projects and technologies, and synopses of visits to international sites are all highlighted in this semiannual journal. Focus on EM programs in this issue is on international collaboration in vitrification projects. Technology highlights covers: in situ sealing for contaminated sites; and remote sensors for toxic pollutants. Section on profiles of countries includes: Arctic contamination by the former Soviet Union, and EM activities with Germany--cooperative arrangements.

  3. Os efeitos do treino musical sobre a cognição numérica e a memória operacional: um estudo prospectivo em crianças pré-escolares

    OpenAIRE

    Silva, Eder Ricardo da [UNESP

    2016-01-01

    Estudos demonstram que a música e o Treino Musical (TM) atuam em diversas áreas do desenvolvimento humano: cognição, linguagem, socialização e raciocínio lógico-matemático. Há evidências de que o Treino Musical propicia ganhos quanto à memória operacional e à numerosidade de crianças escolares. Supõe-se que o TM poderia contribuir para a estimulação destas habilidades cognitivas em crianças pré-escolares. Este estudo prospectivo investigou os efeitos do TM sobre as habilidades de numerosidade...

  4. Desempenho lumínico em edificação escolar com estratégias sustentáveis e bioclimáticas : um estudo de caso

    OpenAIRE

    Christiane Cunha Krum

    2011-01-01

    Frente ao desenfreado desenvolvimento da sociedade nas suas diversas esferas, busca-se novas maneiras de habitar o planeta, de forma a preservá-lo para as futuras gerações. Uma das vertentes nas quais o homem pode intervir e, assim, ajudar a preservar os recursos naturais do planeta, diz respeito à busca por economia energética. Em edificações de uso prolongado e onde são desenvolvidas atividades visuais intensas, o uso da luz natural é de extrema valia, não apenas resultando em economia de e...

  5. Helmintofauna gastrointestinal e hepática do ratinho-caseiro (Mus musculus domesticus) do Arquipélago da Madeira : potencial zoonótico e importância em sanidade animal

    OpenAIRE

    Valente, Ana Sofia Bonito de Figueiredo

    2016-01-01

    Dissertação de Mestrado Integrado em Medicina Veterinária Os roedores são reservatórios de diversas espécies parasitas com um importante impacto em saúde humana e animal. O ratinho-caseiro (Mus musculus domesticus), devido aos seus hábitos comensais e proximidade a populações humanas, é uma das espécies mais problemáticas no que respeita a disseminação de parasitas zoonóticos. Nas ilhas da Madeira e de Porto Santo, este risco é amplificado pelas elevadas densidades populacionais e pela est...

  6. Preparação e caracterização de catalisadores de níquel suportado em óxido de zircônio e aluminio

    OpenAIRE

    Berrocal, Guillermo José Paternina

    2005-01-01

    Níquel suportado em alumina tem sido extensivamente usado para catalisar a reforma a vapor do metano, devido ao seu baixo custo, quando comparado a outros catalisadores, à base de metais nobres. Entretanto, ele não é estável durante a reação principalmente devido à transição de fase e à sinterização do suporte. Isto requer o desenvolvimento de novos catalisadores. Uma opção atrativa seria usar suportes baseados em zircônia, que possui diversas vantagens, tais como estabilidade térmica, dureza...

  7. INCIDÊNCIA DE CASOS E RECIDIVAS DE ERLIQUIOSE EM UMA CLÍNICA VETERINÁRIA PARTICULAR, ENTRE 2011 E 2012, NO MUNICÍPIO DE ITUVERAVA-SP

    OpenAIRE

    Rosa, Ezequias Patrocinio; Faculdade Dr. Francisco Maeda - FAFRAM; Rosa, Ezequias Patrocínio; Faculdade Dr. Francisco Maeda - FAFRAM

    2014-01-01

    A erliquiose canina,também conhecida popularmente como “doença do carrapato”, é uma doença infecciosa endêmica em diversas regiões do Brasil. Atualmente,vem apresentando elevada prevalência, principalmente em locais onde o clima é propício para o desenvolvimento de seu agente transmissor, o carrapato Rhipicephalus sanguineus. Seu agente etiológico é um micro-organismo da família Anaplasmatacea, do gênero Ehrlichia, parasita intracelular obrigatório que infecta os leucócitos, principalmente o...

  8. Estudo comparativo entre estrelas centrais de nebulosas planetárias deficientes em hidrogênio

    Science.gov (United States)

    Marcolino, W. L. F.; de Araújo, F. X.

    2003-08-01

    Apresentamos neste trabalho o resultado de um estudo das principais características espectrais das estrelas centrais de nebulosas planetárias (ECNP) deficientes em hidrogênio. A origem e a evolução dessas estrelas ainda constitui um problema em aberto na evolução estelar. Geralmente esses objetos são divididos em [WCE], [WCL] e [WELS]. Os tipos [WCE] e [WCL] apresentam um espectro típico de uma estrela Wolf-Rayet carbonada de população I e as [WELS] apresentam linhas fracas de carbono e oxigênio em emissão. Existem evidências que apontam a seguinte sequência evolutiva : [WCL] = > [WCE] = > [WELS] = > PG 1159 (pré anã-branca). No entanto, tal cenário apresenta falhas como por exemplo a falta de ECNP entre os tipos [WCL] e [WCE]. Baseados em uma amostra de 24 objetos obtida no telescópio de 1.52m em La Silla, Chile (acordo ESO/ON), ao longo do ano 2000, apresentamos os resultados da comparação das larguras equivalentes de diversas linhas relevantes entre os tipos [WCL], [WCE] e [WELS]. Verificamos que nossos dados estão de acordo com a sequência evolutiva. Baseado nas linhas de C IV, conseguimos dividir pela primeira vez as [WELS] em dois grupos principais. Além disso, os dados reforçam a afirmação de que as [WCE] são as estrelas que possuem a maior temperatura entre as ECNP deficientes em hidrogênio. Discutimos ainda, a escassez de dados disponíveis na literatura e a necessidade da obtenção de parametros físicos para estes objetos.

  9. Mitochondrial cytochrome <em>B> sequence divergence among Spanish, Alpine and Abruzzo chamois (genus <em>Rupicapra>

    Directory of Open Access Journals (Sweden)

    Nadia Mucci

    1998-12-01

    Full Text Available Abstract We have studied genetic divergence and phylogenetic relationships of Alpine, Spanish and Abruzzo chamois (genus <em>Rupicapra> by sequencing a region of 330 nucleotides within the mitochondrial DNA cytochrome <em>b> gene (mtDNA cyt <em>b>. These sequences were aligned with additional homologous sequences of Caprinae: Japanese serow, Chinese goral, Canadian mountain goat, Mishmi takin, muskox, Sardinian mouflon and domestic goat. Results suggest that, using representatives of the Bovini as outgroups, the Caprinae constitute a monophyletic clade. However, inferred phylogenetic relationships among and within tribes of Caprinae were poorly defined and did not reflect current evolutionary and taxonomical views. In fact, the Asian Rupicaprini goral and serow constituted a strongly supported clade, which included the muskox, while the takin grouped with <em>Ovis>. Therefore, the monophyly of Ovibovini was not supported by cyt <em>b> sequences. Species of <em>Rupicapra> joined a strongly supported monophyletic clade, which was distantly related to the Asian rupicaprins and <em>Oreamnos>. Therefore, the monophyly of the Rupicaprini was not supported by these cyt <em>b> sequences. There were sister species relationships within <em>Rupicapra>, Spanish and Alpine chamois and the Abruzzo chamois (<em>Rupicapra pyrenaica ornataem> was strictly related to the Spanish chamois (<em>Rupicapra pyrenaica parvaem>, as previously suggested by allozyme data and biogeographic reconstructions.

  10. Cryptosporidium infection in Brazil: implications for veterinary medicine and public health Infecção por Cryptosporidium no Brasil: implicações em medicina veterinária e em saúde pública

    Directory of Open Access Journals (Sweden)

    Marcelo Vasconcelos Meireles

    2010-12-01

    Full Text Available The aim of this review paper is to report the results of cryptosporidiosis research in Brazil, mainly its occurrence in animals and implications for veterinary medicine and public health. An increasing number of papers related to Cryptosporidium spp. infection in Brazil are available at national and international literature. The main focus described in these papers is the occurrence of Cryptosporidium spp. in food, environmental samples, in humans and several animal species, particularly birds, cattle, dogs and cats. Using molecular biology techniques, most Cryptosporidium species and genotypes identified in other countries have been described in Brazil. In mammals, there are descriptions of infection by C. bovis, C. canis, C. felis, C. meleagridis, C. parvum, and the cervine genotype; in birds, the following species and genotypes have been described: C. baileyi, C. galli, C. meleagridis, C. parvum and the avian genotypes I, II and III. Several species have been described in humans, such as C. parvum, C. hominis, and some species adapted to animal hosts such as C. canis, C. felis and C. meleagridis.O objetivo deste trabalho foi relatar, por meio de revisão de literatura, os resultados de pesquisas sobre a criptosporidiose no Brasil, com ênfase em sua ocorrência em animais e suas implicações em medicina veterinária e em saúde pública. Um número crescente de trabalhos sobre a infecção por Cryptosporidium spp. no Brasil está disponível na literatura nacional e internacional. Nestes trabalhos, são abordados principalmente aspectos relacionados à ocorrência de Cryptosporidium spp. em alimentos, amostras ambientais, no homem e em diversas espécies animais, particularmente em aves, bovinos, cães e gatos. Por meio de técnicas de biologia molecular, a maioria das espécies e alguns genótipos identificados em outros países foram descritos no Brasil. Em mamíferos, houve identificação de C. bovis, C. canis, C. felis, C. meleagridis

  11. New Trifluoromethyl Triazolopyrimidines as Anti-<em>Plasmodium> <em>falciparum> Agents

    Directory of Open Access Journals (Sweden)

    Núbia Boechat

    2012-07-01

    Full Text Available According to the World Health Organization, half of the World’s population, approximately 3.3 billion people, is at risk for developing malaria. Nearly 700,000 deaths each year are associated with the disease. Control of the disease in humans still relies on chemotherapy. Drug resistance is a limiting factor, and the search for new drugs is important. We have designed and synthesized new 2-(trifluoromethyl[1,2,4]triazolo[1,5-<em>a>]pyrimidine derivatives based on bioisosteric replacement of functional groups on the anti-malarial compounds mefloquine and amodiaquine. This approach enabled us to investigate the impact of: (i ring bioisosteric replacement; (ii a CF3 group substituted at the 2-position of the [1,2,4]triazolo[1,5-<em>a>]pyrimidine scaffold and (iii a range of amines as substituents at the 7-position of the of heterocyclic ring; on <em>in vitroem> activity against <em>Plasmodium falciparumem>. According to docking simulations, the synthesized compounds are able to interact with <em>P. falciparumem> dihydroorotate dehydrogenase (<em>Pf>DHODH through strong hydrogen bonds. The presence of a trifluoromethyl group at the 2-position of the [1,2,4]triazolo[1,5-<em>a>]pyrimidine ring led to increased drug activity. Thirteen compounds were found to be active, with IC50 values ranging from 0.023 to 20 µM in the anti-HRP2 and hypoxanthine assays. The selectivity index (SI of the most active derivatives 5, 8, 11 and 16 was found to vary from 1,003 to 18,478.

  12. A quebra do espelho: sexualidade e identidade em Hamlet

    Directory of Open Access Journals (Sweden)

    Leny André Pimenta

    2017-12-01

    Full Text Available Este artigo propõe refletir sobre as questões da sexualidade e identidadeem Hamlet. Ocorpus de análise é um recorte de três cenas do RPG digital “Mabinogi: Hamlet – ‘To be, or not to be’. O suporte digital constitui-se de um hibridismo de linguagens e este provoca uma (reconfiguração educacional, pois aciona no jogador digital e leitor habilidades e competências diferentes da mídia impressa. Sabe-se que a sexualidade tem sido problematizada, ao longo da história, por meio da literatura e das reflexões que se inscrevem em diferentes linguagens culturais, a partir das diversas práticas e discursos circulantes, em que se produzem possibilidades de subjetivação. Diante do exposto pretende-se ir ao encontro de inquietações que geram algumas questões: A literatura, inscrita em um suporte digital, favorece a “experiência de si” em relação ao cotidiano da sexualidade humana? O ódio do desejo é o que move a quebra do espelho para o reconhecimento de si? O aparato teórico-metodológico dessa reflexão assenta-se na perspectiva discursiva francesa e no viés da psicanálise lacaniana. Observa-se que o sujeito pode, por meio da literatura, ser levado a observar a si mesmo, analisar-se, decifrar-se, reconhecendo-se como um domínio de sua verdade, e nesse sentido a sua subjetividade constrói seu entendimento do que seja sexualidade ou ressalta os recalques dos desejos incompreendidos.

  13. Efeitos da facilitação neuromuscular proprioceptiva na estabilidade postural e risco de quedas em pacientes com sequela de acidente vascular encefálico: estudo piloto Efectos de la facilitación neuromuscular propioceptiva en la estabilidad postural y riesgo de caídas en pacientes con secuela de accidente vascular encefálico: estudio piloto Effects of proprioceptive neuromuscular facilitation in postural stability and risk of falls in patients with sequelae of stroke: pilot study

    OpenAIRE

    Natália Noman de Lacerda; Érika Baptista Gomes; Hudson Azevedo Pinheiro

    2013-01-01

    O controle do tronco é uma habilidade motora básica necessária para executar diversas tarefas funcionais, e é deficiente em pacientes que sofreram acidente vascular encefálico (AVE). Objetivo: Avaliar o efeito do método facilitação neuromuscular proprioceptiva (PNF) na estabilidade postural e risco de quedas em pacientes com sequela de AVE. Metodologia: Foi realizado estudo de intervenção que consistiu em treinamento da estabilidade postural por meio de um protocolo fixo constituído por 5 exe...

  14. Estudos sobre a Esquistosomose em Pernambuco, Brasil

    Directory of Open Access Journals (Sweden)

    Aggeu Magalhães

    1940-01-01

    Full Text Available Os autores referem os resultados dos estudos sobre esquistosomose realisados, em Pernambuco, de Novembro de 1938 a Dezembro de 1939. Não se extendem em discussão, nem comentarios, tampouco, relacionam suas verificações com o que já foi relatado sobre o assunto em trabalhos nacionais e estrangeiros, pelo fato de que, não se trata de um resultado definitivo e sim de atividades que irão proseguir. Os estudos sistematicos em torno da endemia esquistosomica foram executados em duas localidades diversas pela população e pela situação geografica e ecologica. Pontezinha é uma povoação de 1200 habitantes localizada proximo a um conjunto de charcos e lagõas de agua dôce onde pululam caramujos do tipo olivaceus de Spix, e apresenta uma taxa de infestação de 21,4% para individuos do sexo masculino, e de 14,7% para os do sexo feminino. A distribuição por grupos de idade mostra que a incidencia cresce até o grupo de 16 a 20 anos de idade, quando atinge a percentagem de 37,8%, para decrescer em seguida. Em Vitoria a incidencia foi maior nos indivíduos do sexo feminino, atingindo a taxa de 41,4%, dando o sexo masculino a taxa de 36,9%. Por idade a percentagem maxima é atingida pelo grupo de 11 a 15 anos. Vitoria é uma cidade de 1500 habitantes, construida á margem do rio Tapacurá, onde se encontram numerosos caramujos do tipo centimetralis Lutz. A divergencia de incidencia corre por conta dos habitos da população e pela utilização que ela faz do rio, sendo maior em Vitoria porque a proximidade do rio facilita o uso de suas aguas para banho e serviços domesticos, condicionando este ultimo fato, a maior infestação das mulheres. O estudo da frequencia da infestação dos caramujos por cercarias de diversos trematodios, permite suspeitar uma relação inversa entre a taxa de infestação e o diametro maximo atingido pelos caramujos, parecendo este fato confirmar os trabalhos de Vianna Martins sobre a identidade dos hospedeiros

  15. Genotoxicity of <em>Euphorbia hirtaem>: An <em>Allium cepaem> Assay

    Directory of Open Access Journals (Sweden)

    Kwan Yuet Ping

    2012-06-01

    Full Text Available The potential genotoxic effects of methanolic extracts of <em>Euphorbia hirta em>which is commonly used in traditional medicine to treat a variety of diseased conditions including asthma, coughs, diarrhea and dysentery was investigated using <em>Allium cepaem> assay. The extracts of 125, 250, 500 and 1,000 µg/mL were tested on root meristems of <em>A. cepaem>. Ethylmethanesulfonate was used as positive control and distilled water was used as negative control. The result showed that mitotic index decreased as the concentrations of <em>E. hirtaem> extract increased. A dose-dependent increase of chromosome aberrations was also observed. Abnormalities scored were stickiness, c-mitosis, bridges and vagrant chromosomes. Micronucleated cells were also observed at interphase. Result of this study confirmed that the methanol extracts of <em>E. hirta em>exerted significant genotoxic and mitodepressive effects at 1,000 µg/mL.

  16. Prática Baseada em Evidências em Psicologia e a História da Busca pelas Provas Empíricas da Eficácia das Psicoterapias

    Directory of Open Access Journals (Sweden)

    Jan Luiz Leonardi

    Full Text Available Resumo Tradicionalmente, a escolha pelo tipo de intervenção psicoterápica para diferentes quadros clínicos depende fundamentalmente da experiência profissional do terapeuta e de sua predileção por determinadas estratégias clínicas. Todavia, esse cenário tem se modificado no contexto da prática baseada em evidências. Apesar do extenso e acalorado debate na literatura internacional da Psicologia nos últimos anos, o tema da prática baseada em evidências tem sido pouco discutido na Psicologia brasileira. Em vista disso, este artigo tem por objetivo apresentar o paradigma de prática baseada em evidências em Psicologia sob uma perspectiva histórica, enfatizando o embate entre os diferentes posicionamentos sobre o assunto e os esforços da American Psychological Association em elaborar um modelo representativo das diversas perspectivas teóricas, metodológicas, conceituais e práticas com o intuito de preencher a lacuna entre ciência e prática ainda existente na Psicologia Clínica. Espera-se que este artigo possa contribuir para uma maior participação da Psicologia brasileira nesse movimento.

  17. Lobotomia transorbitária: resultados obtidos em 54 pacientes tratados em hospital privado

    Directory of Open Access Journals (Sweden)

    Paulino W. Longo

    1956-12-01

    Full Text Available Os autores apresentam os resultados obtidos com a lobotomia transorbitária em 54 pacientes de um hospital psiquiátrico privado. Fazem, inicialmente, uma revisão da experiência que já obtiveram com as diversas técnicas de lobotomia, salientando as principais indicações do método terapêutico. Apontam, como fatôres de importância para a seleção dos casos, a sintomatologia clínica (conduta anti-social agressiva, intensidade da tensão emocional e produtividade intelectual patológica, a necessidade da cooperação familiar e a escolha da técnica operatória. Descrevem a técnica da lobotomia transorbitária, modificada por Freeman e analisam o material clínico constante de 54 casos que puderam ser observados durante mais de 6 meses, dos 70 casos já submetidos ao mesmo método, no período de 1953-1955. Os resultados que obtiveram compreendem 16 casos de remissões totais, 10 de remissões parciais, 13 de boas melhoras, 14 inalterados e 1 falecido em conseqüência da operação. Avaliados os resultados de acôrdo com as possibilidades de readaptação familiar, social e de trabalho, os autores relatam que 21 pacientes obtiveram alta e retomaram suas atividades anteriores, 18 permanecem no lar sem atividade útil, 14 continuam internados. O método proporcionou 72,1% de resultados favoráveis, dos quais 38% com reintegração social. As neuroses obsessivas, as depressões ansiosas e as esquizofrenias paranóides constituíram os quadros clínicos melhor influenciados.

  18. Polinização artificial da atemóia com diversas fontes de pólen comparada com a natural Natural and artificial pollination of Atemoya in Brazil

    Directory of Open Access Journals (Sweden)

    Marcelo Rosa Melo

    2002-12-01

    Full Text Available Para estudar a polinização da atemóia (Annona squamosa L. x A. cherimola e determinar a técnica de polinização que produz frutos em maior quantidade e melhor qualidade, montou-se um experimento de polinização. O experimento foi realizado com a cultivar Gefner, no período de novembro de 2000 a março de 2001, em pomar comercial, localizado em Lins (SP, a 424 metros de altitude. As plantas apresentavam 13 anos de idade. Empregou-se o delineamento de blocos ao acaso, com cinco tratamentos e seis repetições. Os tratamentos foram os seguintes: 1 polinização manual (pólen de A. squamosa L.; 2 polinização manual (pólen da atemóia cultivar Gefner; 3 polinização manual (pólen da atemóia cultivar African Pride; 4 polinização manual (pólen da atemóia cultivar PR-3; 5 polinização natural ou aberta. A avaliação do vingamento dos frutos ocorreu dez dias após a polinização e da quantidade dos frutos com conformação perfeita ou defeituosa, aos 40 dias. Os frutos foram colhidos 120 dias após a polinização e pesados individualmente. Amostraram-se, também, três frutos para as seguintes determinações: massa de sementes e da polpa, número de sementes por 100 gramas de polpa, teor de sólidos solúveis totais, acidez total e relação sólidos solúveis/acidez. Para a atemóia, a polinização com pólen de A. squamosa L. foi mais efetiva, aumentando o vingamento de frutos (80,5%, comparado com outros quatro tratamentos, que foi menos de 22%, a produção e a porcentagem de frutos perfeitos.An experiment was carried out to study atemoya pollination and to determine the pollination method that produces fruits in larger amount and quality. The experiment was accomplished with cultivar Gefner, in the period of November 2000 to March 2001, in a commercial orchard located in the county of Lins - SP, at an altitude of 424 meters. The plants were 13 years old. The experimental design was in randomized blocks, with 5 treatments and

  19. Ensino de BIM em curso de graduação em engenharia civil em uma universidade dos EUA: estudo de caso

    Directory of Open Access Journals (Sweden)

    Priscilla Elisa de Azevedo Basto

    Full Text Available Resumo Com o crescente avanço da tecnologia, os empreendimentos da construção civil se tornam cada vez mais complexos. Como forma de acompanhar esse processo, foi desenvolvido o paradigma denominado de Building Information Modeling (BIM, que se baseia na colaboração entre as equipes para a elaboração de um modelo integrado e 3D da edificação do qual serão retiradas as informações necessárias para a gestão do empreendimento. O BIM vem sendo utilizado por diversas empresas, devido aos benefícios potenciais, entretanto sua difusão continua sendo restrita em razão do fato de ser dominado por pequena quantidade de profissionais. Dessa forma, este trabalho objetiva descrever um método de abordagem do ensino de BIM, permitindo a sua avaliação para potencial difusão e utilização, e demonstrar a importância desse método. Para isso foram realizadas a descrição detalhada e a análise da experiência de ensino do BIM na disciplina de Gerenciamento de Projeto da Construção I, ministrada na Arizona State University (ASU, localizada nos Estados Unidos. Através dos resultados obtidos, constatou-se que a disciplina realiza a abordagem do BIM em conjunto com os outros assuntos, pode ser classificada como de nível intermediário, formando um analista BIM, e se mostrou efetiva em apresentar os principais conceitos e usos do BIM aos discentes.

  20. OV-Wav: um novo pacote para análise multiescalar em astronomia

    Science.gov (United States)

    Pereira, D. N. E.; Rabaça, C. R.

    2003-08-01

    Wavelets e outras formas de análise multiescalar têm sido amplamente empregadas em diversas áreas do conhecimento, sendo reconhecidamente superiores a técnicas mais tradicionais, como as análises de Fourier e de Gabor, em certas aplicações. Embora a teoria dos wavelets tenha começado a ser elaborada há quase trinta anos, seu impacto no estudo de imagens astronômicas tem sido pequeno até bem recentemente. Apresentamos um conjunto de programas desenvolvidos ao longo dos últimos três anos no Observatório do Valongo/UFRJ que possibilitam aplicar essa poderosa ferramenta a problemas comuns em astronomia, como a remoção de ruído, a detecção hierárquica de fontes e a modelagem de objetos com perfis de brilho arbitrários em condições não ideais. Este pacote, desenvolvido para execução em plataforma IDL, teve sua primeira versão concluída recentemente e está sendo disponibilizado à comunidade científica de forma aberta. Mostramos também resultados de testes controlados ao quais submetemos os programas, com a sua aplicação a imagens artificiais, com resultados satisfatórios. Algumas aplicações astrofísicas foram estudadas com o uso do pacote, em caráter experimental, incluindo a análise da componente de luz difusa em grupos compactos de galáxias de Hickson e o estudo de subestruturas de nebulosas planetárias no espaço multiescalar.

  1. Mediações em leitura: encontros na sala de aula

    Directory of Open Access Journals (Sweden)

    Adair de Aguiar Neitzel

    Full Text Available Resumo: Trata das mediações em leitura na escola e analisa como o Programa Institucional de Bolsas de Iniciação à Docência (Pibid de Letras da Universidade do Vale do Itajaí (Univali, cujo foco é a formação de leitores no ensino médio, qualifica os futuros professores de língua e literatura a serem mediadores em leitura. A pesquisa foi de abordagem qualitativa, cujo instrumento de coleta de dados foram os portfólios produzidos pelos licenciandos durante o desenvolvimento de um projeto realizado com alunos do 1º e do 3º ano do ensino médio em duas escolas de Itajaí/Santa Catarina. Os dados coletados foram discutidos à luz das concepções de literatura de Lajolo (2001, Calvino (1993, Petit (2009 e Barthes (2007. Os resultados indicam que: a as mediações em leitura promovidas pelo Pibid de Letras têm como concepção que a literatura tem uma função estética, a ser preservada pela escola, pois cabe ao professor ser um mediador entre aluno e literatura, além de possibilitar experiências de apreciação da obra, de fruição estética, que estreitem as relações entre obra e leitor; b as mediações em leitura possibilitadas pelos pibidianos instigaram os alunos ao ato de ler por apreciação; c os pibidianos exercitaram o papel de mediadores em leitura proporcionando aos alunos encontros com o texto literário; d os pibidianos, futuros professores de língua e literatura, ampliaram seu repertório literário e exploraram diversas possibilidades de tratamento fruitivo da literatura, qualificando-se para atuar como mediadores em leitura.

  2. Nefrectomia videolaparoscópica em Graxaim-do-campo (Pseudalopex gymnocercus com displasia renal

    Directory of Open Access Journals (Sweden)

    João P.S. Feranti

    2014-08-01

    Full Text Available Graxains-do-campo habitam o centro-leste da América do Sul, desde o sudeste do Brasil e leste da Bolívia. A displasia renal compreende um espectro de anomalias e é frequentemente relatada em cães de diversas raças, sendo a maioria dos relatos achados de necropsia. A maior parte das doenças renais congênitas nos cães apresenta caráter progressivo, portanto o tratamento é limitado e direcionado a fim de retardar a progressão da doença renal estabelecida. No presente relato é descrita a realização de nefrectomia laparoscópica em graxaim-do-campo (Pseudalopex gymnocercus com displasia renal. Suspeitou-se de doença no rim direito por meio de ultrassonografia e urografia excretora. Para o procedimento cirúrgico foram utilizados três portais (10, 10, 5mm, endoscópio rígido de 10mm/0(0 e clipes para hemostasia dos vasos renais. A técnica cirúrgica utilizada neste canídeo selvagem foi semelhante à usada em cão doméstico, devido à escassez de relatos em cirurgias em graxaim e a inexistência de descrições de nefrectomia videolaparoscópica em graxaim-do-campo. O animal foi acompanhado por um período de 30 dias pós-cirurgia sem a ocorrência de complicações. O presente relato demonstra que a técnica proposta foi adequada para a realização de nefrectomia videolaparoscópica em Graxaim-do-campo.

  3. PROCESSOS FLUVIAIS E MORFOLOGIA EM CONFLUÊNCIAS DE CANAIS: UMA REVISÃO

    Directory of Open Access Journals (Sweden)

    Vanessa Cristina Dos Santos

    2017-03-01

    Full Text Available As confluências de canais constituem ambientes com alterações contínuas na estrutura de fluxo e no transporte de sedimentos. São locais na rede de drenagem em que se operam complexas interações entre matéria e energia, proporcionadas pela combinação de fluxos de padrões diferentes. Esta dinâmica peculiar pode influenciar desde aspectos ecológicos até questões de planejamento espacial e de ocupação do espaço. Nas últimas décadas, o interesse científico sobre os processos geomorfológicos ocorridos em confluências de canais tem aumentado. Este interesse deriva da crescente consciência de que os processos fluviais e a morfologia das confluências resumem as características da bacia hidrográfica a montante, ao mesmo tempo em que desempenham importante papel na regulação da circulação da água e sedimentos na rede de drenagem a jusante. Diversos estudos sobre os processos fluviais e morfologia em confluências de canais envolvem experiências laboratoriais, investigação de campo e simulações. Frequentemente estes estudos são uma combinação de diversas disciplinas como geomorfologia, ecologia, engenharia, hidrologia e sedimentologia, evidenciando o caráter multidisciplinar da análise desses ambientes complexos. No Brasil, os estudos sobre os processos geomorfológicos em confluências de canais são poucos, e em sua maioria desenvolvidas em rios de grande porte. Este artigo traz uma revisão e discussão sobre o estado atual do conhecimento sobre confluências de canais.

  4. Solubilidade das oleoresinas de calêndula (Calendula officinalis L.) e cavalinha (Equisetum arvense) em CO2 supercrítico

    OpenAIRE

    Danielski, Leandro

    2002-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Curso de Pós-Graduação em Engenharia de Alimentos Determinação da solubilidade dos extratos de duas plantas medicinais (calêndula e cavalinha) em dióxido de carbono supercrítico em diversas condições de extração. Compara dados obtidos experimentalmente com dados estimados através do Modelo de Chrastil e da equação de estado de Peng-Robinson. Ênfase também é dada às composições dos extratos obtidos. Cons...

  5. Imunonutrição em colite experimental: efeitos benéficos dos ácidos graxos ômega-3

    OpenAIRE

    CAMPOS,Fábio Guilherme; WAITZBERG,Dan L.; LOGULO,Angela Flávia; TORRINHAS,Raquel Susana; TEIXEIRA,William Gemio Jacobsen; HABR-GAMA,Angelita

    2002-01-01

    Racional - Trabalhos recentes têm enfatizado os benefícios da imunonutrição com ácidos graxos ômega-3 (AGn-3) em diversas situações clínicas. O presente estudo apresenta os resultados da administração parenteral de diferentes emulsões lipídicas em colite aguda experimental e revê a literatura pertinente. Métodos - Setenta e quatro ratos Wistar machos adultos foram randomizados em seis grupos, cinco dos quais submetidos a indução de colite com ácido acético a 10%. Os ratos receberam dieta oral...

  6. Ocorrência de afídeos causando danos em mangueira (Mangifera indica L., no Vale do São Francisco

    Directory of Open Access Journals (Sweden)

    FERREIRA RACHEL GONÇALVES

    2002-01-01

    Full Text Available Observaram-se em pomares de mangueiras (Mangifera indica L., no semi-árido nordestino, altas infestações de afídeos causando danos às folhas novas e brotações da planta. Diversas amostras foram coletadas, tendo sido identificadas três espécies: Aphis craccivora, Aphis gossypii e Toxoptera aurantii. As duas primeiras espécies estão sendo relatadas pela primeira vez como pragas, em mangueiras, no Brasil. Espécimens de referência encontram-se depositados no Laboratório de Entomologia da Embrapa Semi-Árido, localizada em Petrolina-PE.

  7. Caso de Ensino: Abertura de Capital e Implantação de Sistema de Governança Corporativa em uma Empresa Familiar

    Directory of Open Access Journals (Sweden)

    Isaac Gezer Silva de Oliveira

    2015-01-01

    Full Text Available Prime Hospitality Group (PHG é uma empresa familiar,fundada há 17 anos pelo Sr. Milton Albuquerque, com hotéis eresorts espalhados em diversas regiões do País. Recentemente,a companhia decidiu realizar uma abertura de capital naBM&FBOVESPA. Entretanto, a realização de um IPO motivadiversas alterações na estrutura da empresa. Essas mudançasgeram custos e alteram a visão dos membros da família edos diversos stakeholders envolvidos no processo. O presentecaso propõe uma acertiva do impacto da implantação de ummecanismo de governança corporativa, em virtude da realizaçãoda abertura de capital em uma empresa familiar.

  8. Avaliação de algoritmos de escalonamento de disco com qualidade de serviço em ambientes virtualizados

    OpenAIRE

    Souza, Josiney de

    2014-01-01

    Resumo: Virtualização e uma técnica utilizada em diversas áreas de conhecimento, cada qual com seu objetivo específico. Porém todas possuem um ponto em comum: definem a virtualização como uma forma de abstrair um ambiente físico em um ambiente lógico. O foco deste trabalho e a virtualização de servidores. No contexto de virtualização de servidores, máquinas virtuais (VMs) possuem estudos bem definidos para os componentes processador, memória e rede. O componente disco ainda é alvo de pesquisa...

  9. Diagnóstico sorológico e caracterização molecular do vírus da hepatite E em suínos no estado do Pará, Brasil

    OpenAIRE

    SOUZA, Alex Junior Souza de

    2011-01-01

    O vírus da Hepatite E (HEV) é um RNA-vírus entericamente transmissível do gênero Hepevirus causador de hepatite aguda em humanos que apresenta ampla distribuição em diversas regiões do mundo. Suínos são relatados como a principal fonte de infecção para humanos relacionadas aos genótipos 3 e 4 em regiões consideradas não-endêmicas. Neste sentido, o presente estudo teve como objetivo demonstrar a infecção pelo HEV em suínos no Estado do Pará através de métodos sorológicos e moleculares aplic...

  10. Efeitos dos esteróides anabólicos-androgênicos (EAA) associados ao exercício de carga no remodelamento do tendão de Aquiles em ratos

    OpenAIRE

    Rita de Cássia Marqueti

    2005-01-01

    O uso indiscriminado de esteróides anabólicos-androgênicos (EAA) tem sido descrito em diversas situações como causadores de patologias em tendão. O objetivo deste trabalho foi avaliar o remodelamento de tendão em ratos tratados com EAA associando exercício de alta intensidade. Os animais foram divididos em quatro grupos: Grupo I (GI) (sedentário); GII (não treinado, mas recebia injeção de EAA); GIII (treinado sem EAA) and GIV (treinado e também com injeção de EAA). O treinamento foi constituí...

  11. Emergency Medical Service (EMS) Stations

    Data.gov (United States)

    Kansas Data Access and Support Center — EMS Locations in Kansas The EMS stations dataset consists of any location where emergency medical services (EMS) personnel are stationed or based out of, or where...

  12. Reabilitação funcional em lesões vértebro-medulares â A intervenção tradicional e as novas abordagens em fisioterapia

    OpenAIRE

    Patrícia Maria Duarte de Almeida

    2006-01-01

    Resumo: Após uma lesão vértebro-medular, em que existe perda da função de diversas estruturas e sistemas do corpo, resultando em limitações da actividade e restrições da participação social, o objectivo final do processo de reabilitação é a reintegração social com o máximo de funcionalidade e qualidade de vida que o tipo e extensão da lesão permitem. Para atingir este estádio, é necessário um período de reabilitação dentro de um equipa multidisciplinar na qual se inse...

  13. Bloqueio de plexo braquial em um bugio-ruivo (Alouatta guariba: relato de caso

    Directory of Open Access Journals (Sweden)

    E.R. Santos

    Full Text Available RESUMO Os bloqueios regionais vêm sendo explorados e difundidos no dia a dia da medicina veterinária. O presente trabalho relata a execução do bloqueio de plexo braquial em um bugio-ruivo (Alouatta guariba submetido à amputação de três dígitos. Foi utilizada ropivacaína para o bloqueio, com o paciente sob anestesia geral, com auxílio de neuroestimulador periférico. Quatro horas após a cirurgia e cinco horas após o bloqueio, o paciente não apresentava desconforto ou evitava a manipulação do membro operado e não foram observadas complicações. O bloqueio do plexo braquial é mais comumente utilizado quando se pretende promover analgesia e relaxamento muscular do membro torácico em procedimentos cirúrgicos distais à articulação escápulo-umeral. Há carência em estudos anatômicos sobre diversos primatas, incluindo o bugio-ruivo (Alouatta guariba. Apesar da crescente expansão da clínica de animais silvestres, ainda há lacunas no que diz respeito à execução de técnicas anestésicas em diversas espécies.

  14. A manipulacao de prematuros em uma Unidade de Terapia Intensiva Neonatal

    Directory of Open Access Journals (Sweden)

    Fabiola Lima Pereira

    2013-12-01

    Full Text Available Os prematuros são submetidos a diversas manipulações e procedimentos durante internação em unidades neonatais, com consequências deletérias para a saúde. O objetivo do presente estudo foi descrever a manipulação a que são submetidos os prematuros durante as 24 horas em uma unidade de terapia intensiva neonatal (UTIN. Estudo observacional, descritivo, exploratório, realizado com 20 prematuros, filmados continuamente por um período de 24 horas, de setembro de 2008 a março de 2009 numa UTIN. Os prematuros foram submetidos a uma média de 768 manipulações e 1.341 procedimentos. A manipulação durou em média 2 horas e 26 minutos em um período de 24 horas. Cada manipulação agrupou uma média de 2,2 procedimentos, a maioria no turno matutino As manipulações isoladas representaram 65,6% do total de manipulações e a maioria teve duração inferior a um minuto. Conclui-se que nas 24 horas avaliadas, os prematuros foram submetidos a um excesso de manipulações na UTIN.

  15. Topiramato: uma experiência em crianças com epilepsia parcial

    Directory of Open Access Journals (Sweden)

    Rocha Cristiane

    2001-01-01

    Full Text Available Estudamos a resposta clínica ao topiramato (TPM em crianças abaixo de 15 anos de idade. O tratamento foi iniciado com doses de 12,5 mg/kg/dia (1-7 mg/kg/dia, com aumentos semanais de 12,5 mg. Foram estudadadas 11 crianças, 9 do sexo feminino e 2 do masculino, de 3 a 14 anos de idade, com epilepsia parcial associada a etiologias diversas. Apenas um paciente teve dor abdominal intensa. Após o início do TPM, um paciente teve controle total das crises, 5 tiveram melhora maior que 75% na frequência delas, 1 paciente apresentou melhora em 50% e 3 não tiveram controle. Ao final, verificamos controle adequado das crises mesmo em quadros mais graves, com doses de até 100 mg/dia do TPM e em associação a carbamazepina, na maioria dos casos.

  16. O julgamento moral de dilemas éticos em negociação

    Directory of Open Access Journals (Sweden)

    Filipe João Bera de Azevedo Sobral

    2009-10-01

    Full Text Available Os processos cognitivos da tomada de decisão ética têm sido objeto de estudo de diversas pesquisas interessadas em compreender o que motiva os executivos a tomar as decisões eticamente questionáveis. A negociação é uma das práticas empresariais mais interessantes para se estudar a honestidade e a ética, uma vez que é central a todas as interações humanas e é rica em dilemas éticos. Esta pesquisatem como objetivo analisar em que medida diferenças individuais em termos de prioridades do sistema de valores condicionam o julgamento moral de práticas negociais eticamente ambíguas. Usando um modelo de equações estruturais, as relações entre os sistemas de valores, a ideologia ética e o julgamento moral são avaliadas. Os resultados sugerem que as dimensões da individualidade humana analisadas exercem uma influência significativa na forma como os gestoresavaliam a moralidade de práticas negociais eticamente ambíguas. Os resultados podem ainda contribuir significativamente para melhorar a compreensão do processo de tomada de decisão ética.

  17. EVIDÊNCIAS COMPARATIVAS EM EMPRESAS BRASILEIRAS E ESTRANGEIRAS NA TRANSFERÊNCIA DE CONHECIMENTO NO FRANCHISING

    Directory of Open Access Journals (Sweden)

    Rogério Stival Morgado

    2011-01-01

    Full Text Available O objetivo deste trabalho consiste em comparar a transferência de conhecimento no franchising em empresas brasileiras e estrangeiras atuantes no Brasil. O franchising tem-se demonstrado um fenômeno importante em vários países, evidenciando considerável crescimento em âmbito global. Ele se caracteriza como um novo tipo de organização empresarial, tornando-se, recentemente, alvo de diversas pesquisas acadêmicas, pois oferece amplo campo de análise. Contudo, nota-se que a temática ainda sofre com limitações quanto a referências bibliográficas e estudos empíricos. Além disso, constata-se a existência de uma lacuna acerca de estudos que focam na realidade de países que não pertencem ao mainstream e de estudos comparados. Para o alcance do objetivo proposto, adotou-se a abordagem qualitativa através da aplicação de entrevistas em profundidade. Os resultados apontam que as empresas brasileiras pertencentes ao setor de franquias orientam sua transferência de conhecimento em processos baseados em conhecimento tácito, utilizando menor número de mecanismos de transferência nesses processos. Já as empresas estrangeiras, por sua vez, apoiam-se em processos formais e mecanismos de suporte ao processo de transferência como reuniões periódicas, treinamentos e recursos tecnológicos, repercutindo em maior grau de controle sobre o processo de transferência de conhecimento para suas unidades franqueadas.

  18. Avaliação qualitativa em medicina: experiência em propedêutica médica na UFBA, 2003

    OpenAIRE

    Araújo,Dolores; Peixinho,André Luiz

    2006-01-01

    No momento atual, reconhece-se a necessidade de a educação médica promover mudanças de modo a formar profissionais com um perfil mais humanizado. Para alcançar tal propósito, é essencial investir em transformações no campo da avaliação. O principal objetivo deste trabalho é descrever as diversas funções do diário de campo como instrumento de avaliação educacional. Trata-se de estudo de caso qualitativo, que envolve a leitura de diários de campo de alunos matriculados nos semestres 2003.1 e 20...

  19. Aplicação de um Sistema Imunológico Artificial em um Tower Defense

    Directory of Open Access Journals (Sweden)

    Renato de Pontes Pereira

    2015-07-01

    Full Text Available Um dos problemas enfrentados na criação da inteligência artificial em um jogo é a resposta mecânica e desequilibrada do computador às ações do jogador. Diversas técnicas podem ser usadas para evitar ou amenizar isso. Por exemplo, com técnicas bio-inspiradas como redes neurais artificiais, algoritmos genéticos ou sistemas imunológicos artificiais. Esse artigo apresenta a área de sistemas imunológicos artificiais, uma demonstração da aplicação dessa técnica em um jogo de Tower Defense.

  20. Deficiência de ferro e anemia em escolares da área rural de Novo Cruzeiro (Minas Gerais) Brasil.

    OpenAIRE

    Rezende, Eliane Garcia; Santos, Margarete Aparecida; Lamounier, Joel Alves; Galvão, Márcio Antônio Moreira; Leite, Romário Cerqueira

    2008-01-01

    O reconhecimento da relação existente entre a deficiência de ferro e a presença ou ausência de anemia, comprometendo diversas funções do organismo humano, tem sido tema de debates na área de nutrição em âmbito internacional, especialmente por ser uma das deficiências mais prevalentes no mundo. Objetivo: este estudo descreve a ocorrência de deficiência de ferro e busca elucidar a frequência de anemia ferropriva em escolares de área rural. Casuística e Métodos: foram avaliados níveis de hemoglo...

  1. Burnout e sua relação com o clima organizacional em funcionários de um hospital

    OpenAIRE

    Sônia Maria Pessoto dos Santos; Vanessa de Sousa; Fabián Javier Marín Rueda

    2015-01-01

    Introdução: Em contextos em que os contatos interpessoais são intensos, os profissionais estão sujeitos a diversas situações de estresse. A síndrome de burnout advém do contato com estressores interpessoais crônicos provenientes do ambiente de trabalho, e seu desenvolvimento é influenciado por alguns aspectos, entre eles, os socioambientais. Objetivo: Entender como se dá a interação entre os sintomas da síndrome de burnout e algumas variáveis do clima organizacional no ambiente hospitalar. Mé...

  2. O estatuto da imagem-pulsão em Cronicamente Inviável e Amarelo Manga

    Directory of Open Access Journals (Sweden)

    Alexandre Rocha da Silva

    2014-01-01

    Full Text Available Este artigo retoma o debate inaugurado por Gilles Deleuze sobre a imagem-pulsão e avalia como tais imagens se fazem presentes no cinema brasileiro contemporâneo. O trabalho recua até Sigmund Freud para explicitar o conceito de pulsão e até Herbert Marcuse para pensar o engajamento das pulsões nas relações socioculturais. Neste percurso, descreve-se uma nova forma de pensamento no cinema a partir do conceito. No contexto do cinema brasileiro, identificou-se que não há um filme constituído unicamente por imagens-pulsão, em lugar disso, elas se encontram dispersas em cenas de diferentes filmes, desempenhando funções diversas nas relações entre mundos originários e derivados.

  3. O Sentido de "Estranho" em Leslie Silko e Kaka Werá Jecupé: Identidades Culturais, Deslocamento e Terra

    Directory of Open Access Journals (Sweden)

    Lúcia Helena Azevedo Vilela

    2002-12-01

    Full Text Available Análise da narrativa da trajetória pessoal de personagens que revelam sua perplexidade enquanto sujeitos indígenas diante de experiências de deslocamentos forçados e perda da ligação com a terra e com o lar. O estudo é realizado à luz do conceito de “estranho” ou estranhamento de Homi Bhabha e focaliza a personagem revolucionária, Angelita La Ecapía, em Almanac of the Dead, e o narrador de Todas as vezes que dissemos adeus. O texto procura enfatizar elementos que fazem emergir a força política da narrativa ao revelarem que luta pelo espaço entre fronteiras e diversas esferas de ação ocasiona sentimentos contraditórios e muitas vezes traumáticos para os sujeitos em questão.

  4. Turismo de saúde em Portugal: turismo médico e turismo de bem-estar

    OpenAIRE

    Ferreira, Ricardo

    2013-01-01

    Mestre em Gestão de Serviços de Saúde O turismo de saúde engloba duas subcategorias, o turismo médico (cirurgias e serviços de saúde no estrangeiro) e o turismo de bem-estar (viajar para locais específicos para promoção da saúde de uma forma preventiva). Na vertente de bem-estar, há vários aspectos a considerar e diversas formas de promoção da saúde de forma preventiva, uma delas é o turismo termal (com um longo histórico em Portugal mas actualmente numa situação premente de reforma). A...

  5. Epidemias em escala mundial e no Brasil Epidemics on a global scale and in Brazil

    Directory of Open Access Journals (Sweden)

    José da Rocha Carvalheiro

    2008-12-01

    Full Text Available Epidemias devem ser consideradas não como um tipo especial de doença, mas sim como parte de um complexo processo de distribuição de patologias no tempo e no espaço. Diversas classificações de doenças existem, sendo oficial a CID atualmente em sua décima versão. As cartas de controle, empregadas pelo serviço de saúde, permitem uma aproximação à fase, endêmica ou epidêmica, em que se encontram especialmente doenças sujeitas a vigilância em todo o mundo, as quarentenáveis. O conceito de surto deve ser encarado como um indício de concentração de episódios que merece estudo mais profundo. As mais importantes doenças transmissíveis no mundo em desenvolvimento são chamadas negligenciadas e merecem atenção especial das agências de fomento de pesquisa por não serem tidas como prioritárias pela indústria farmacêutica. No Brasil, a Agenda de Prioridades de Pesquisa em Saúde inclui diversas doenças dessa natureza e tem sido contempladas em editais do CNPq e do Ministério da Saúde.Epidemics must not be seen as a special kind of disease but as part of a complex process of distribution of pathologies over time and space. Among the different existing disease classifications, the ICD, currently in its 10th edition, is the official one. The control charts being employed by the health system allow estimating the stage of development, endemic or epidemic, of diseases, especially those subject to worldwide control, the quarantinable diseases. The concept outbreak should be understood as a signal for the concentration of episodes worth being studied in depth. The most important communicable diseases in the developing world, the so-called neglected diseases, deserve special attention from the research funding agencies for not ranging among the priorities of the pharmaceutical industry. In Brazil, the agenda of priorities in health research includes various diseases of this nature, which have been contemplated in calls for projects

  6. Bases da terapia celular em cardiologia Cell therapy in cardiology

    Directory of Open Access Journals (Sweden)

    Antonio Carlos C. Carvalho

    2009-05-01

    Full Text Available O implante de células para o tratamento de doenças cardiovasculares encontra-se sob investigação em vários centros no mundo. Várias linhagens celulares, de células-tronco bem caracterizadas a frações contendo diferentes tipos de células, têm sido investigadas em modelos animais. Apesar dos avanços obtidos na última década, na área de ciência básica, com relação a esta nova modalidade terapêutica, diversas questões permanecem sem resposta. Pouco ainda se sabe sobre os mecanismos através dos quais a terapia celular possa gerar resultados efetivos. Adicionalmente, a melhor via para o transplante, o número total e a concentração de células, e o melhor tipo celular permanecem questões importantes, ainda sem definição. É fato de que diversas células da medula óssea exercem seus efeitos através de mecanismos parácrinos e de que existe um complexo mecanismo de interação, contato e liberação de sinais entre essas células e as outras populações celulares nos órgãos lesados. Atualmente, a maioria dos estudos em humanos se concentra em células de origem adulta e autóloga, em oposição ao uso de células de origem embrionária. Esta revisão analisa os principais ensaios clínicos que utilizaram células derivadas de medula óssea em quatro cardiopatias: doença arterial coronariana aguda e crônica, e nas cardiomiopatias chagásica e dilatada. Os resultados desses estudos demonstram que o procedimento é seguro e exequível, e potencialmente eficaz. Inquestionavelmente, mais estudos pré-clínicos e clínicos são necessários para acessar o real potencial benefício desse novo modelo terapêutico.Cell transplantation for the treatment of cardiovascular diseases is being investigated in many centers throughout the world. Various cell lines, from well characterized stem cells to cell fractions containing different types of cells, have been investigated in animal models. Despite progress in the basic research of

  7. As várias formas de amar e o nosso trabalho ou sobre amores decentes e o nosso trabalho Las diversas formas de amar son nuestro trabajo The several ways of loving and our work

    Directory of Open Access Journals (Sweden)

    Flávia Regina Souza Ramos

    2004-08-01

    Full Text Available O texto se propõe a refletir sobre duas novas expressões relativas ao trabalho: a expressão trabalho decente, situada em discursos e projetos de governo, e a expressão worklover, encontrada no espaço acadêmico e suas formas de divulgação. Com vistas a uma motivação e problematização inicial do tema de modo articulado ao contexto do cotidiano do trabalho de enfermagem, o texto se apóia na simbolização alegórica captada a partir de imagens e personagens do cinema ficcional, que estimulam a relação entre as experiências de amor e de trabalho como processo de autoreflexividade.El texto se propone reflexionar sobre dos nuevas expresiones relativas al trabajo: la expresión trabajo decente, situada en discursos y proyectos de gobierno, y la expresión worklover, encontrada en el espacio académico y sus formas de divulgación. Con vistas a una motivación y problematización inicial del tema de modo articulado al contexto del cotidiano trabajo de enfermería, el texto se apoya en la simbologia alegórica captada a partir de imagenes y ppersonajes del cine de ficción, que estimulan la relación entre las esperiencias de amor y de trabajo como proceso de autoreflexión.This text aims at proposing a reflection on two new work-related terms: decent work, used in speeches and government projects, and worklover, found in the academic space and its forms of divulgation. Aiming at an early motivation and problematization of the topic in connection with the nursing work day-to-day context, the text is supported by the allegorical symbolization captured in images and characters of fiction cinema, which encourage the relationship between love and work experiences as a self-reflection process.

  8. International EMS Systems

    DEFF Research Database (Denmark)

    Langhelle, Audun; Lossius, Hans Morten; Silfvast, Tom

    2004-01-01

    Emergency medicine service (EMS) systems in the five Nordic countries have more similarities than differences. One similarity is the involvement of anaesthesiologists as pre-hospital physicians and their strong participation for all critically ill and injured patients in-hospital. Discrepancies do...... exist, however, especially within the ground and air ambulance service, and the EMS systems face several challenges. Main problems and challenges emphasized by the authors are: (1) Denmark: the dispatch centres are presently not under medical control and are without a national criteria based system...... is the only country that has emergency medicine (EM) as a recognised speciality but there is a need for more fully trained specialists in EM; (4) Norway: the ordinary ground ambulance is pointed out as the weakest link in the EM chain and a health reform demands extensive co-operation between the new health...

  9. Saúde em retrospectiva e prospectivas

    Directory of Open Access Journals (Sweden)

    Paula Borges Jacques

    2017-12-01

    sobre consumo alimentar e sua relação com doenças infecciosas(13; como fator de risco para câncer de intestino(14; e ainda a diferença entre homens e mulheres(15, ficando o alerta do perigo da ingesta exagerada de doces, alimentos processados, gordurosos e lanches não saudáveis para a saúde populacional. A promoção de saúde fundamenta-se em realizar atividades de modificação dos hábitos e costumes humanos, e a educação em saúde representa uma aliada fundamental nesse percurso, sendo vista na RBPS, por exemplo, sua importância com residentes de lares de adoção na cidade de Santiago, Rio Grande do Sul(16, ou sua atuação através da saúde bucal(17. Para gestantes, recém-nascidos, crianças, adolescentes, jovens, adultos e idosos, a RBPS semeou atenção à saúde em suas páginas também neste ano, seja por meio das revisões de literatura, seja pelos dados epidemiológicos e relatos de experiência aqui divulgados. O ano foi profícuo e rico em informações de promoção à saúde a todas as faixas etárias. Não deixou passar esquecido o bem-estar de surdos, homens pós-infarto, hipertensos, diabéticos, pacientes internados em UTIs, internos de abrigos, cuidadores ou moradores de todas as regiões brasileiras e de outros países, de comunidades quilombolas ou de Cabo Verde. A RBPS apresenta resultados de estudos relevantes e eficientes, contribuindo para o papel de efetivar a promoção de saúde em suas páginas, trazendo discussões embriagantes sobre essa temática aos leitores de todas as áreas da saúde e afins. Finalmente, a RBPS continuará focada em contribuir positivamente para o fortalecimento da promoção da saúde coletiva e individual, ao publicar o produto advindo de representantes das mais diversas áreas da saúde, onde uns aprendem e apreendem com os outros, repercutindo no crescimento tanto da sua própria área quanto da saúde em geral.

  10. DANOS SOCIOAMBIENTAIS URBANOS EM CURITIBA: UMA ABORDAGEM GEOGRÁFICA

    Directory of Open Access Journals (Sweden)

    Denise Pinheiro FRANCISCO

    2005-11-01

    Full Text Available Classificam-se os danos socioambientais urbanos em Curitiba como provenientes de: Atividades Noturnas, Empreendimentos Imobiliários, Atividades Industriais, Serviços de Transportes e Atividades Diversas. Estas atividades e serviços compõem o conjunto de intervenções objeto de pesquisa em 44 ações civis públicas propostas pelo Ministério Público do Paraná (de 1986 a 2002. Os danos socioambientais urbanos identificados em Curitiba são: poluição sonora, poluição atmosférica, poluição hídrica, degradação ambiental e desequilíbrio ecológico, com danos à fauna e à flora e riscos à qualidade de vida (saúde e bemestar, além da lesão ao patrimônio cultural de valor histórico, urbanístico e paisagístico. Urban partner-ambient damages in Curitiba: a geographic boarding Abstract The urban partner-environmental damages in Curitiba are classified as: Evening activities, Real Estate Enterprises, Industrial Activities, Services of Transports and Diverse Activities. These activities and services compose the set of interventions which were the object of research in 44 civil actions public proposals for the Public prosecution service of the Paraná State (1986−2002. The identified urban partnerambient damages in Curitiba are: sonorous pollution, atmospheric pollution, hydrical pollution, environmental degradation and ecological disequilibrium, with damages to the fauna and flora and risks to the quality of life (health and well-being, beyond the injury to the cultural patrimony of historical, urban and landscape value.

  11. VALOR NUTRICIONAL DE PLEUROTUS DJAMOR CULTIVADO EM PALHA DE BANANEIRA

    Directory of Open Access Journals (Sweden)

    Jamile Rosa RAMPINELLI

    2010-11-01

    Full Text Available

    Cogumelos do gênero Pleurotus representam um alimento de custo baixo, com teor elevado de proteínas, aminoácidos essenciais, proporção elevada de ácidos graxos insaturados, diversas vitaminas e minerais, além de teores baixos de gorduras, ácidos nucléicos, açúcares e calorias. Assim, o objetivo deste trabalho foi avaliar o valor nutricional de basidiomas de Pleurotus djamor de 1o e 2o fluxo produtivo, cultivados em palha de bananeira, em termos de teores de carboidratos, proteínas, fi bras, gorduras, cinzas, vitaminas, fósforo e potássio. Os teores de carboidratos totais, proteína bruta, fi bra bruta e cinzas diminuíram do 1o para o 2o fl uxo produtivo de 32,7 para 27,4g/100g, de 20,5 para 19,8g/100g, de 22,4 para 12,7g/100g e de 7,4 para 6,3g/100g, respectivamente. Os valores de gordura bruta e umidade não variaram, permanecendo em torno de 1,1 e 90g/100g, respectivamente. Os teores de vitamina B1 foram superiores aos de vitamina B2, independentemente do fluxo produtivo, e foi encontrada maior quantidade de potássio do que de fósforo. Pleurotus djamor, de 1o fl uxo produtivo, pode ser considerado fonte de fósforo e potássio, além de apresentar baixo teor de açúcar e não conter gordura.

  12. Caracterização do conforto acústico em edifícios de habitação

    OpenAIRE

    Anjos, Miguel António Remechido

    2013-01-01

    A presente dissertação tem como objectivo realizar uma avaliação da percepção do conforto acústico no interior das habitações, pelos seus habitantes. Estes dados subjectivos foram posteriormente relacionados com os descritores físicos utilizados para a caracterização do desempenho acústico de elementos construtivos. Para as amostras seleccionadas, três empreendimentos em Lisboa, os resultados acústicos dos mesmos foram obtidos pela análise de diversas notas técnicas, realizadas pelo departame...

  13. A rela??o entre os estilos de socializa??o materna e as concep??es de perd?o em adolescentes.

    OpenAIRE

    Santos, Lydia Maria Sena e

    2014-01-01

    O presente estudo buscou analisar a rela??o entre a percep??o dos adolescentes sobre os estilos de socializa??o materna e concep??es sobre perd?o. Os estilos de socializa??o parental s?o definidos como padr?es de atua??o caracter?sticos, que os pais utilizam em diversas situa??es do cotidiano. Esses padr?es caracterizam-se por um conjunto de t?cnicas e formas de atua??o dos pais, que abrangem desde atitudes de aceita??o at? atitudes de controle dos comportamentos indesejados, al?m da imposi??...

  14. Avaliação da histopatologia hepática em boga (Chondrostoma polylepis) e barbo (Barbus bocagei) capturados no Rio Vizela

    OpenAIRE

    Teixeira, Paulo Alexandre Nunes

    2008-01-01

    Dissertação de Mestrado em Tecnologias Ambientais Os ecossistemas aquáticos recebem quantidades consideráveis de poluentes que levam ao declínio da qualidade da água e podem afectar de um modo adverso a saúde dos diferentes organismos aquáticos. As fontes de contaminantes dos ecossistemas aquáticos são diversas e incluem os poluentes de origem agrícola, industrial e doméstica, podendo afectar os peixes de diferentes formas. Nesta perspectiva, a biomonitorização ambiental recorrendo a bioma...

  15. A verdade (des)construída: a inserção da homossexualidade na comunidade cristã Nova Esperança, em Natal

    OpenAIRE

    Lima, Carlos Chagas Vilela

    2013-01-01

    A pluralidade religiosa tem sido cada vez mais intensa no território brasileiro, ao passo que tem servido como objeto de estudo em diversas áreas do conhecimento. Neste contexto, esta dissertação tem como objetivo geral apontar a heterogeneidade vivenciada no meio protestante e o crescimento das denominações evangélicas, notadamente aquelas que se declaram inclusivas por atender à diversidade humana, buscando entender a relação entre religião e homossexualidade. Como objetivo e...

  16. Metodologia para processamento distribuído com aplicação em representação polinomial de superfícies

    OpenAIRE

    Abrantes, Rossini Pena

    2008-01-01

    Pesquisas em diversas áreas do conhecimento estão envolvendo cada vez mais informações a serem processadas e demandam mais poder computacional para processar tais informações. Por esta razão, a área de Processamento Distribuído é uma área fundamental por possibilitar que grandes demandas de poder de processamento sejam supridas. Com base neste fato, o presente trabalho propõe uma metodologia para distribuição de processamento através da Internet utilizando computação voluntária. A distribuiçã...

  17. Análise do efeito diurno do fator de visão do céu no microclima e nos níveis de conforto térmico em ruas de pedestres em Curitiba

    Directory of Open Access Journals (Sweden)

    Flavia Cristina Osaku Minella

    Full Text Available O clima urbano é resultado das ações de diversas variáveis, entre elas o fator de visão do céu (FVC. O objetivo deste artigo é analisar o efeito diurno do FVC no microclima e nos níveis de conforto térmico em ruas de pedestre em Curitiba. Foram realizadas 13 medições, em 15 pontos com diferentes características urbanas, ao longo de quatro ruas de pedestres. Os resultados mostram baixa correlação entre o microclima e o FVC. Porém, foi possível verificar a relação existente entre a configuração urbana, a temperatura radiante média e a radiação solar. Além disso, é possível perceber a relação entre o FVC e os índices de conforto térmico PMV e PET. Para a situação de verão e em pontos com menor obstrução do céu, obteve-se maior desconforto para calor. No entanto, esses mesmos pontos podem apresentar melhor situação de conforto em dias com temperaturas mais baixas.

  18. Estudo qualitativo da integração ensino-serviço em um curso de graduação em Odontologia

    Directory of Open Access Journals (Sweden)

    Ana Luiza de Souza

    Full Text Available Este estudo, de abordagem qualitativa, investigou, por meio de entrevistas individuais e em grupo, a integração ensino-serviço entre uma universidade pública do sul do Brasil e os serviços públicos de saúde. Os dados foram analisados sob a Análise Temática de Conteúdo, e, na análise, emergiram dois eixos temáticos e quatro categorias. O eixo temático Ativador agrupou aspectos desenvolvidos no programa e o eixo em Construção revelou as dificuldades observadas. As categorias identificadas foram: Parceria Ensino-Serviço; Estrutura Curricular; Mudança de Paradigma e Práticas Pedagógicas. Conclui-se que os participantes compreendem a integração ensino-serviço como potencial estratégia colaboradora do processo de mudança de práticas na formação em saúde, apesar de haver grupos resistentes. O processo de mudança de práticas se desenvolveu ativamente a partir da reestruturação curricular. A integração ensino-serviço é um dos eixos que busca solidificar a proposta curricular, por meio de ações diversas na interface do ensino com o serviço.

  19. EFEITOS DA TERAPIA LASER DE BAIXA INTENSIDADE EM MODELO EXPERIMENTAL DE TENDINOPATIA EM RATOS: REVISÃO DE LITERATURA

    Directory of Open Access Journals (Sweden)

    Diego Rodrigues Pessoa

    2017-12-01

    Full Text Available Tendinopatia é o termo utilizado para descrever lesões que afetam os tendões (tendinites e tendinoses, caracterizada pela presença de inflamação e degeneração tecidual, associada à sobrecarga mecânica e a movimentos repetitivos. Diversas modalidades terapêuticas têm sido estudadas no tratamento das tendinopatias, entre elas, destaca-se a terapia a laser de baixa intensidade (TLBI que visa acelerar o processo regenerativo, diminuir a inflamação e reduzir a dor.  Diante do exposto, este trabalho teve como objetivo realizar revisão de literatura, de artigos científicos com foco nos efeitos da terapia a laser de baixa intensidade (TLBI, em modelo experimental de tendinopatia em ratos. O estudo baseou-se na investigação de publicações entre janeiro de 2011 a outubro de 2016, em bases de dados eletrônicas com o emprego dos descritores “tendinopatia”, “laserterapia”, “ratos”, “tendão” e “terapia a laser de baixa intensidade”, combinados aos descritores booleanos. Foram selecionados sete artigos que apontam que a TLBI possibilitou a redução de importantes marcadores pró-inflamatórios, tais como IL-6 e TNF-α, bem como a proliferação aumentada de tenócitos, independentemente da dosagem aplicada. Os estudos científicos selecionados nesta revisão sistemática sinalizaram efeitos positivos da TLBI, principalmente na modulação da resposta inflamatória aguda ou crônica após a indução da tendinopatia.

  20. NARRATIVAS EM CONVERGÊNCIAS: SER-AGIR EM UMA METODOLOGIA COMPLEXA

    Directory of Open Access Journals (Sweden)

    Felipe Gustsack

    2008-07-01

    Full Text Available Esse estudo questiona a suficiência dos aportes teóricos vinculados ao paradigma da complexidade para a emergência de uma metodologia complexa da aprendizagem e demais afazeres do campo da educação. Nossas hipóteses partem da convicção de que tais constructos dão conta dessas especificidades, potencializando o conhecer como um ser-agir em convivência. Procuramos demonstrar essa perspectiva, nesse texto, procedendo à descrição do funcionamento do Grupo de Ações e Investigações Autopoiéticas - GAIA, colocando-nos como sujeitos das ações de pesquisa, como observadores implicados no ato mesmo de observar, o que vincula este estudo às abordagens autopoiéticas e metacognitivas. O desafio é possibilitar, a partir de vivências dialógicas em ambientes complexos como as redes presenciais e virtuais de aprendizagens, novas compreensões acerca da inseparabilidade do conhecer-viver como fluxo passível de aplicação na educação. Articulando pesquisas de diferentes áreas do conhecimento, os pesquisadores envolvidos com o GAIA tendem a convergir na defesa de uma educação que se tece junto; que faz emergir o respeito mútuo, a amorosidade e também a morosidade necessária ao ser-agir na consciência de que cada organismo conspira para a vida com seu próprio ritmo e implicações cósmicas. Nossas conclusões apontam para vínculos significativos entre os recortes teóricos apresentados e a compreensão do observar-agir com um modo de pesquisar e de aprender, tendo como veículos as narrativas do grupo em convergências diversas, processo que nominamos como ontoepistemogênese. Além disso, demonstramos com esse estudo a processualidade das narrativas como fluxos que nos permitem mapear uma metodologia complexa, que emerge e faz emergir o ser-conhecer a partir de padrões que conectam e marcadores conceituais. Palavras-chave: educação; complexidade; linguagem; narrativas; aprendizagem.

  1. Sobrevida de pacientes em hemodiálise em um hospital universitário

    Directory of Open Access Journals (Sweden)

    Fernanda Ismaela Rolim Teixeira

    2015-03-01

    Full Text Available Introdução: A doença renal crônica (DRC apresenta altas taxas de morbidade e mortalidade, especialmente na população em diálise. Objetivo: Determinar a taxa de sobrevida; caracterizar o perfil epidemiológico e clínico; identificar as comorbidades e as variáveis associadas à sobrevida dos pacientes submetidos à hemodiálise. Métodos: Trata-se de um estudo de natureza descritiva e longitudinal constituído por 162 pacientes submetidos à hemodiálise, em um hospital universitário. As variáveis categóricas foram descritas por meio de frequências e porcentagem e as numéricas por meio de média ± desvio padrão. A análise por meio da regressão de Cox foi utilizada para estudar a influência de diversas variáveis clínicas e demográficas. Foram construídas as curvas de sobrevida das variáveis significantes com o método de Kaplan Meyer. Resultados: A idade média foi de 48,09 anos, a renda familiar mensal e o nível de instrução mostraram-se reduzidos na maior parte dos estudados. A hipertensão arterial mostrou-se a principal comorbidade associada à insuficiência renal crônica terminal, seguida pelo diabetes mellitus. Os fatores que comprometeram significativamente a sobrevida dos pacientes foram a idade avançada no início da terapia, a hemoglobina e a albumina. A taxa de sobrevida global foi de 84,71% e 63,32% em um e cinco anos, respectivamente. Conclusão: A taxa de sobrevida foi considerada baixa e mostrou-se decrescente ao longo dos anos, não representando melhorias apesar dos avanços tecnológicos, diagnósticos e terapêuticos. A idade avançada, a hipoalbuminemia e a anemia são fortes preditores de mortalidade.

  2. EFEITOS DO EXERCÍCIO FÍSICO EM COMPORTAMENTOS RELACIONADOS COM A ANSIEDADE EM RATOS

    Directory of Open Access Journals (Sweden)

    Daniel Fernandes Barbosa

    2016-04-01

    Full Text Available Introdução: Os efeitos do exercício físico são associados à redução da ansiedade e à manutenção desse estado por diversas horas. Contudo, poucos trabalhos têm investigado os efeitos do exercício agudo sobre a ansiedade. Objetivo: Investigar o efeito agudo da intensidade do exercício físico sobre comportamento relacionado com a ansiedade em modelos animais. Métodos: Cinquenta e seis ratos Wistar machos foram distribuídos aleatoriamente em oito grupos: controle, 0%, 5% e 50% de carga correspondente ao peso corporal dos animais. Os grupos foram submetidos a um protocolo de natação que consistiu na realização de 30 minutos de exercício para os grupos 0% e 5% e dez sessões de dez saltos intercalados por um minuto de descanso para o grupo 50%. Após a natação, os animais foram expostos aos testes de campo aberto e labirinto em cruz elevado. Os dados foram avaliados estatisticamente pela One-way ANOVA e teste post hoc de Bonferroni, adotando-se P<0,05. Resultados: Na análise dos dados no teste de campo aberto foram encontradas diferenças significativas nas medidas de cruzamento (F(3,20 = 9,60; p < 0,01, ocorrendo um aumento de cruzamentos do grupo 5% (81,2 ± 14,8 com relação ao grupo controle (45,0 ± 8,7. Foi encontrada diferença significativa no percentual de entrada nos braços abertos (F(3,28 = 5,63; p < 0,01, havendo uma diminuição no grupo 50% (25,4 ± 17,0 com relação ao grupo controle (46,8 ± 9,1. Conclusão: A prática de exercício físico na intensidade de 5% apresentou efeitos ansiolíticos, enquanto na intensidade de 50%, apresentou efeitos ansiogênicos.

  3. Avifauna em quatro fazendas madeireiras de Irati-PR: análise para certificação florestal

    Directory of Open Access Journals (Sweden)

    Vânia Rossetto Marcelino

    Full Text Available O presente estudo comparou a comunidade e a diversidade de aves em fragmentos de Floresta Ombrófila Mista de diferentes tamanhos. Para tanto, oito fragmentos divididos em conjuntos, formando duas classes (somando de 20 a 70 ha e de 100 a 420 ha, foram selecionados em quatro fazendas no distrito de Guamirim, Irati-PR. O método de amostragem consistiu em trajetos irregulares percorridos pelas áreas naturais, realizados entre 2009 e 2010, sempre na primavera e no outono. Foram calculadas a Frequência Relativa e a Diversidade mediante o índice de Shannon. As análises MRPP e Cluster, e o teste Mann-Whitney (Wilcoxon W foram utilizados para comparar os fragmentos. Os resultados comprovam que fragmentos maiores abrigam uma comunidade distinta e mais diversa do que manchas menores do que 100 ha. Foram registradas 88 espécies pelos trajetos irregulares. Somando estas às espécies anotadas a partir de observações eventuais em qualquer área dentro das fazendas e nos seus arredores, obteve-se um total de 123 espécies registradas localmente.

  4. Religiosidade Cristã entre Árabes em São Paulo: Desafios no Passado e no Presente

    Directory of Open Access Journals (Sweden)

    Oswaldo Truzzi

    Full Text Available Resumo O artigo discute a religiosidade cristã entre árabes e descendentes em São Paulo, um contingente quase exclusivamente composto em sua origem por sírios e libaneses que integraram as levas migratórias chegadas ao país a partir do final do século XIX. Após contextualizar suas identidades religiosas, o artigo descreve, com base em pesquisas realizadas pelo próprio autor e dados colhidos em publicações diversas, a situação dos diferentes credos cristãos considerando sua constituição histórica, sua construção institucional e os dilemas que caracterizam a manutenção dos adeptos e suas tradições de origem. Elenca ainda os fatores que debilitaram o vigor da identidade religiosa entre descendentes e os desafios enfrentados para angariar fiéis e afirmar uma identidade específica em meio ao catolicismo romano dominante.

  5. UM ESTUDO DA RELAÇÃO ENTRE O TRABALHO EM EQUIPE E A APRENDIZAGEM ORGANIZACIONAL

    Directory of Open Access Journals (Sweden)

    Julia Hosana Santos Franco

    2011-06-01

    Full Text Available O artigo apresenta os resultados de pesquisa realizada acerca do trabalho em equipe e aprendizagem organizacional. Na pesquisa, o objetivo geral foi o de analisar as estratégias utilizadas pela gestão com pessoas para incentivar o trabalho em equipe, visando alcançar êxito no processo de aprendizagem no contexto organizacional. O artigo busca responder ao seguinte problema: quais estratégias são utilizadas pela gestão com pessoas para incentivar o trabalho em equipe visando êxito na aprendizagem organizacional? Para o desenvolvimento da pesquisa, recorreu-se ao estudo do caso da empresa BETA S/A, do setor siderúrgico, que resolveu implantar o trabalho em equipe. Nesse sentido, houve a oportunidade de pesquisarmos os desdobramento da prática e suas decorrências sob a ótica dos empregados das diversas áreas. Dentro do estudo foram realizadas 39 entrevistas com líderes e subordinados. Quanto aos resultados, inicialmente, verificou-se que apesar da literatura defender um perfil inovador e estratégico para a área de RH, considerando suas transformações para a valorização do profissional como alternativa para alcançar melhores resultados organizacionais, identificamos que a prática do trabalho em equipe carece de aprofundamento no estudo de suas iniciativas e conseqüências, sendo inclusive de difícil aplicação em qualquer organização.

  6. Atividades cotidianas de crianças em situação de rua

    Directory of Open Access Journals (Sweden)

    Alves Paola Biasoli

    2002-01-01

    Full Text Available Este estudo apresenta resultados de uma pesquisa descritiva da observação de atividades cotidianas de vinte crianças em situação de rua da área central de Porto Alegre, Brasil. Os dados foram coletados através de uma metodologia observacional elaborada especificamente para utilização em pesquisas no contexto da rua, associada a uma entrevista estruturada para obtenção de dados bio-sócio-demográficos. Os resultados revelam que as crianças utilizam o espaço da rua para diversas atividades, incluindo tarefas que garantem a subsistência pessoal e, às vezes, da família. Foram também observadas brincadeiras solitárias ou em grupo, demonstrando que embora estejam em atividade de trabalho, continuam sendo crianças em desenvolvimento. A discussão dos dados, baseada na Teoria dos Sistemas Ecológicos, salienta a importância de estudos que descrevam os aspectos saudáveis que meninos e meninas em situação de rua podem apresentar neste ambiente. Alternativas de intervenção nesta situação devem enfatizar a participação comunitária e da sociedade civil na efetivação de propostas de apoio sócio-afetivo para estas crianças e suas famílias.

  7. PROBLEMAS LOCOMOTORES EM FRANGOS DE CORTE - REVISÃO. / LOCOMOTOR PROBLEMS IN BROILER CHICKENS - A REVIEW.

    Directory of Open Access Journals (Sweden)

    IBIARA CORREIA DE LIMA ALMEIDA PAZ

    2008-12-01

    Full Text Available O bem estar animal é, sem dúvida, um dos pontos em que os produtores de aves devem se atentar para conseguir melhor rentabilidade e colocação no mercado externo. No entanto, é necessário ter uma ampla idéia de que alguns pontos impostos por mercados importadores, muitas vezes não tem fundamento científico e tratam-se mais de barreiras não tarifárias que de problemas de produção propriamente ditos. Dentre os vários fatores que afetam o bem estar animal pode-se destacar a incidência de problemas locomotores, principalmente em animais confinados. Estes distúrbios podem acarretar em perdas de até 6% em lotes comercias de frangos de corte, além de outras perdas não mensuráveis em linhas de abate, por fraturas e hematomas. Existem diversas metodologias para diagnosticar problemas locomotores, entretanto, a mais difundida na indústria avícola é o Gait Score, por sua facilidade de aplicação e por englobar os diferentes tipos de problemas locomotores. Sabe-se, contudo que esta metodologia é bastante subjetiva e pode inferir  em  diferentes  níveis  de  avaliação  dependendo  do método  utilizado. Os  problemas locomotores devem ser prevenidos já que depois de estabelecidos as perdas são inevitáveis.

  8. Atividades cotidianas de crianças em situação de rua

    Directory of Open Access Journals (Sweden)

    Paola Biasoli Alves

    Full Text Available Este estudo apresenta resultados de uma pesquisa descritiva da observação de atividades cotidianas de vinte crianças em situação de rua da área central de Porto Alegre, Brasil. Os dados foram coletados através de uma metodologia observacional elaborada especificamente para utilização em pesquisas no contexto da rua, associada a uma entrevista estruturada para obtenção de dados bio-sócio-demográficos. Os resultados revelam que as crianças utilizam o espaço da rua para diversas atividades, incluindo tarefas que garantem a subsistência pessoal e, às vezes, da família. Foram também observadas brincadeiras solitárias ou em grupo, demonstrando que embora estejam em atividade de trabalho, continuam sendo crianças em desenvolvimento. A discussão dos dados, baseada na Teoria dos Sistemas Ecológicos, salienta a importância de estudos que descrevam os aspectos saudáveis que meninos e meninas em situação de rua podem apresentar neste ambiente. Alternativas de intervenção nesta situação devem enfatizar a participação comunitária e da sociedade civil na efetivação de propostas de apoio sócio-afetivo para estas crianças e suas famílias.

  9. A influência da prática da ginástica laboral na qualidade de vida de trabalhadores de propriedades produtoras de leite: estudo de caso na comunidade São Justino em Juína/MT

    OpenAIRE

    Cavalheiro, Claudionor Nunes

    2014-01-01

    A ginástica laboral tem sido utilizada em diversas intervenções visando à melhora na qualidade de vida dos trabalhadores de setores urbanos (comercial e industrial), com resultados satisfatórios. Essa melhora ainda é incipiente no setor rural, mesmo sendo um setor importante da economia nacional e em franco desenvolvimento. O presente estudo visou identificar a influência da prática sistemática da ginástica laboral na melhoria da qualidade de vida e da saúde dos trabalhadores de propriedades ...

  10. O ator em jogo

    OpenAIRE

    Daves Otani

    2005-01-01

    Resumo: O Ator em Jogo é uma reflexão pessoal sobre aspectos do processo criativo em dois espetáculos teatrais encenados pelo grupo Boa Companhia: PRIMUS (adaptado do conto "Comunicado a uma Academia", de Franz Kafka) e MISTER K. E OS ARTISTAS DA FOME (adaptado do conto "O artista da fome", de Kafka). Utilizando a experiência prática da capoeira, que serviu como matriz criativa nessas duas montagens, em cada uma a seu modo específico, faço uma reflexão a partir de um diário que relata experiê...

  11. International EMS Systems

    DEFF Research Database (Denmark)

    Langhelle, Audun; Lossius, Hans Morten; Silfvast, Tom

    2004-01-01

    exist, however, especially within the ground and air ambulance service, and the EMS systems face several challenges. Main problems and challenges emphasized by the authors are: (1) Denmark: the dispatch centres are presently not under medical control and are without a national criteria based system...... enterprises to re-establish a nation-wide air ambulance service; (5) Sweden: to create evidence based medicine standards for treatment in emergency medicine, a better integration of all part of the chain of survival, a formalised education in EM and a nation wide physician staffed helicopter EMS (HEMS) cover....

  12. International EMS Systems

    DEFF Research Database (Denmark)

    Langhelle, Audun; Lossius, Hans Morten; Silfvast, Tom

    2004-01-01

    . Access to on-line medical advice of a physician is not available; (2) Finland: the autonomy of the individual municipalities and their responsibility to cover for primary and specialised health care, as well as the EMS, and the lack of supporting or demanding legislation regarding the EMS; (3) Iceland...... exist, however, especially within the ground and air ambulance service, and the EMS systems face several challenges. Main problems and challenges emphasized by the authors are: (1) Denmark: the dispatch centres are presently not under medical control and are without a national criteria based system...

  13. Expression of Selected <em>Ginkgo em>>biloba em>Heat Shock Protein Genes After Cold Treatment Could Be Induced by Other Abiotic Stress

    Directory of Open Access Journals (Sweden)

    Feng Xu

    2012-05-01

    Full Text Available Heat shock proteins (HSPs play various stress-protective roles in plants. In this study, three <em>HSP> genes were isolated from a suppression subtractive hybridization (SSH cDNA library of <em>Ginkgo bilobaem> leaves treated with cold stress. Based on the molecular weight, the three genes were designated <em>GbHSP16.8em>, <em>GbHSP17em> and <em>GbHSP70em>. The full length of the three genes were predicted to encode three polypeptide chains containing 149 amino acids (Aa, 152 Aa, and 657 Aa, and their corresponding molecular weights were predicted as follows: 16.67 kDa, 17.39 kDa, and 71.81 kDa respectively. The three genes exhibited distinctive expression patterns in different organs or development stages. <em>GbHSP16.8em> and <em>GbHSP70em> showed high expression levels in leaves and a low level in gynoecia, <em>GbHSP17em> showed a higher transcription in stamens and lower level in fruit. This result indicates that <em>GbHSP16.8em> and <em>GbHSP70 em>may play important roles in <em>Ginkgo> leaf development and photosynthesis, and <em>GbHSP17em> may play a positive role in pollen maturation. All three <em>GbHSPs> were up-regulated under cold stress, whereas extreme heat stress only caused up-regulation of <em>GbHSP70em>, UV-B treatment resulted in up-regulation of <em>GbHSP16.8em> and <em>GbHSP17em>, wounding treatment resulted in up-regulation of <em>GbHSP16.8em> and <em>GbHSP70em>, and abscisic acid (ABA treatment caused up-regulation of <em>GbHSP70em> primarily.

  14. Dois tratados plotinianos em Eusébio de Cesaréia

    OpenAIRE

    Goulet-Cazé, Marie-Odile

    2010-01-01

    Eusébio de Cesaréia, na sua Preparação Evangélica, cita um longo trecho do escrito de Plotino ao qual Porfírio, na sua edição das  Enéadas, intitulou “Sobre a imortalidade da alma” (IV. 7 [2]). Surpreendentemente, esse trecho citado por Eusébio (hoje em dia editado como os capítulos 8.1-8.5 desse tratado) está ausente da edição porfiriana. Tentou-se explicar essa ausência de diversas formas: alguns estudiosos pensam que o texto citado por Eusébio seja um resquício da edição que Eustóquio fize...

  15. Editorial - Número temático Manejo do fogo em áreas protegidas

    OpenAIRE

    Ribeiro, Katia Torres; ICMBio; França, Helena; UFABC; Miranda, Heloísa Sinátora; UnB; Berlinck, Christian Niel; ICMBio

    2011-01-01

    Neste segundo número de Biodiversidade Brasileira trazemos ampla e variada discussão sobre o manejo do fogo em áreas protegidas. Abrimos assim a segunda linha editorial da revista, que visa à publicação dos resultados técnico-científicos do processo de avaliação do estado de conservação das espécies bem como, na forma de números temáticos, a discussão, entre pesquisadores de diversas áreas e gestores, de temas críticos relacionados à conservação e ao manejo. A dimensão alcançada pelos incêndi...

  16. Avaliacao de interacoes medicamentosas potenciais em prescricoes da atencao primaria de Vitoria da Conquista (BA), Brasil

    OpenAIRE

    Danyllo Fabio Lessa Leao; Cristiano Soares de Moura; Danielle Souto de Medeiros

    2014-01-01

    As interações medicamentosas são fatores de risco para a ocorrência de reações adversas a medicamentos. Este estudo teve o objetivo de avaliar as interações medicamentosas potenciais em prescrições da atenção primária de Vitória da Conquista (BA), visando preencher a lacuna de conhecimento sobre essa temática no Brasil. Foram coletadas informações sobre diversas variáveis de prescrições oriundas da atenção primária e as interações medicamentosas avaliadas a partir dos bancos de dados do Medsc...

  17. Relato de pesquisa: relatos baseados em estudos científicos

    OpenAIRE

    Soares, César Pedrosa

    2014-01-01

    Resumo A dependência química corresponde a um fenômeno que é divulgado e discutido por diversas áreas da nossa sociedade, visto que o uso abusivo de substâncias psicoativas tornou-se um problema social e de saúde pública. Este trabalho apresenta como objetivo observar a visão das pessoas diante dos dependentes químicos levando em conta a relação da variável resiliência, que é um conjunto de processos sociais e intrapsíquicos que permitem o desenvolvimento saudável do indivíduo, com as vari...

  18. Interacoes medicamentosas potenciais em pacientes com doenca renal cronica

    Directory of Open Access Journals (Sweden)

    Alessandra Batista Marquito

    2014-03-01

    Full Text Available Introdução: Interações medicamentosas (IMs são frequentes na prática clínica e estão diretamente relacionadas a fatores como polifarmácia, idade avançada e deficiência na metabolização e excreção de fármacos. Indivíduos com doença renal crônica (DRC comumente utilizam diversas classes de medicamentos, constituindo um importante grupo de risco para IMs. Objetivo: Identificar potenciais interações entre medicamentos prescritos a renais crônicos em tratamento conservador, e fatores associados a sua ocorrência. Métodos: Estudo observacional transversal, com análise de 558 prescrições. O potencial interativo dos medicamentos foi traçado tendo como suporte a base de dados MICROMEDEX®, software que disponibiliza farmacopeias conhecidas internacionalmente. Resultados: Houve predomínio de indivíduos do sexo masculino (54,7%, idosos (69,4%, no estágio 3 da DRC (47,5%, com sobrepeso e obesos (66,7%. As comorbidades mais prevalentes foram a hipertensão arterial sistêmica (68,5% e o diabetes mellitus (31,9%. IMs potenciais foram detectadas em 74,9% das prescrições. De um total de 1.364 IMs detectadas, 229 (16,8% foram de gravidade maior e 5 (0,4% contraindicadas, com necessidade de intervenção imediata. Interações de gravidade moderada ou menor foram identificadas respectivamente em 1.049 (76,9% e 81 (5,9% das prescrições. Observou-se que a probabilidade de ocorrência de uma IM aumentou em 2,5 vezes para cada medicamento adicional (IC = 2,18-3,03. Obesidade, diabetes, hipertensão e estágio avançado da DRC foram fatores de risco fortemente associados para ocorrência de IM. Conclusão: A associação de medicamentos em indivíduos com DRC relacionou-se com alta prevalência de IMs potencialmente graves, especialmente nos estágios mais avançados da doença.

  19. “INTERCULTURALIDADE – EMIGRANTES PORTUGUESES EM LONDRES”

    Directory of Open Access Journals (Sweden)

    Ana Maria Romão

    2015-12-01

    Full Text Available O presente artigo insere-se no projeto “Empreendedorismo Emigrante Português em Londres, Nice, Mónaco e Andorra”. É financiado pela FCT, sendo a instituição proponente o CEPESE/Universidade do Porto. Este projeto integra uma equipa multidisciplinar e tem por base a análise comparativa do percurso empreendedor de emigrantes portugueses, a sua inserção nos contextos socioeconómicos, culturais e políticos locais e a sua presença nos media (e.g., internet, documentários, imprensa. O mesmo respeita a três situações territoriais distintas: a cidade de Londres (Reino Unido, o principado de Andorra, a Região dos Alpes Marítimos (mais especificamente a cidade de Nice e o Principado do Mónaco. Pretende-se com este artigo estudar a relação dos emigrantes portugueses, em Londres, com as comunidades autóctones e com outros emigrantes de outras culturas em cruzamento nesta cidade. A relação que se estabelece nesta amálgama de pertenças sociais diversificadas, nas quais eles próprios e as novas gerações, seus filhos, hesitam entre assumirem ou rejeitarem estas diversas influências. Esta relação nem sempre é fluída, essencialmente devido ao receio que os pais têm de que os seus filhos assumam comportamentos desviantes em relação à sua cultura de origem, provocando tensões dentro da própria família. A metodologia utilizada centra-se na análise qualitativa de vinte entrevistas individuais, realizadas na cidade de Londres entre os dias 1 e 10 de Julho de 2014. Cada entrevista, com a duração de 15 a 60 minutos, foi orientada por um guião previamente definido. Com o presente estudo pretendemos contribuir para uma melhor abordagem do fenómeno da emigração, analisando as suas implicações/estratégias interculturais em contexto familiar.

  20. ESTIMULAÇÃO ELÉTRICA NERVOSA TRANSCUTÂNEA DO NERVO TIBIAL POSTERIOR COMO FORMA DE TRATAMENTO EM PACIENTES COM INCONTINÊNCIA FECAL DECORRENTE DA PRÁTICA DO SEXO ANAL EM HOMOSSEXUAIS DO SEXO MASCULINO: UMA REVISÃO BIBLIOGRÁFICA

    OpenAIRE

    Takahachi, Márcia; Fagundes, Diego Santos; Uesugui, Helena Meika; de Mattos Dias, Cláudio Gellis; Vidal Ramos, Luiz Armando; Calixto, Paulo Henrique Matayoshi; Trindade, Fábio Rodrigues; Silva, Alceu dos Santos; Fecury, Amanda Alves; Sá de Oliveira, Júlio Cesar

    2014-01-01

    A Incontinência Fecal é uma desordem caracterizada por uma liberação involuntária tanto de gases como fezes líquidas e sólidas pelo reto, sendo marcada pela incapacidade fisiológica de manter controle sobre armazenamento e expulsão das fezes em locais socialmente adequados. Sua etiologia possui diversas variáveis, sendo de relevância a este estudo, os fatores traumáticos determinados pela penetração de objetos durante o sexo ou auto-estimulação anal, assim como o traumatismo ano-retal gerado ...