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Sample records for affect serum lipids

  1. Supplemental barley protein and casein similarly affect serum lipids in hypercholesterolemic women and men.

    Science.gov (United States)

    Jenkins, David J A; Srichaikul, Korbua; Wong, Julia M W; Kendall, Cyril W C; Bashyam, Balachandran; Vidgen, Edward; Lamarche, Benoicirct; Rao, A Venketeshwer; Jones, Peter J H; Josse, Robert G; Jackson, Chung-Ja C; Ng, Vivian; Leong, Tracy; Leiter, Lawrence A

    2010-09-01

    High-protein diets have been advocated for weight loss and the treatment of diabetes. Yet animal protein sources are often high in saturated fat and cholesterol. Vegetable protein sources, by contrast, are low in saturated fat and without associated cholesterol. We have therefore assessed the effect on serum lipids of raising the protein intake by 5% using a cereal protein, barley protein, as part of a standard therapeutic diet. Twenty-three hypercholesterolemic men and postmenopausal women completed a randomized crossover study comparing a bread enriched with either barley protein or calcium caseinate [30 g protein, 8374 kJ (2000 kcal)] taken separately as two 1-mo treatment phases with a minimum 2-wk washout. Body weight and diet history were collected weekly during each treatment. Fasting blood samples were obtained at wk 0, 2, and 4. Palatability, satiety, and compliance were similar for both the barley protein- and casein-enriched breads, with no differences between the treatments in effects on serum LDL cholesterol or C-reactive protein, measures of oxidative stress, or blood pressure. Nevertheless, because no adverse effects were observed on cardiovascular risk factors, barley protein remains an additional option for raising the protein content of the diet. PMID:20668250

  2. SERUM LIPIDS IN ANXIETY NEUROSIS

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    T K Mishra; Shankar, R.; Sharma, I.; P.K. Srivastava

    1984-01-01

    SUMMARY Serum cholesterol, total triglycerides, HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol, free cholesterol and total phospholipids were studied in 36 patients of anxiety neurosis and 24 control subjects. Serum triglycerides, VLDL-cholesterol and free-cholesterol were found to be significantly raised while esterified cholesterol WJS significantly lowered in anxiety neurosis. A significant negative correlation was observed between the anxiety score and free cholesterol in ferrule pati...

  3. Effects of taurine intake on serum lipids in young women

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    Sadako Matsui

    2015-04-01

    Full Text Available Background: Taurine is an abundant amino acid in human cells, promoting ocular and biliary health, which is also used to treat congestive heart failure, hypertension, and hepatitis. Recently, taurine-enriched energy drinks have become popular with young adults, but the effects of taurine on serum lipids in young adults are unknown. Objective: We studied the influence of oral administration of taurine on serum lipid levels in healthy young women. Methods: Ten healthy young women with a mean body mass index of 20.0kg/m2, apolipoprotein E (apoE phenotype 3/3 and normal menstrual cycles participated. Each subject was instructed to orally ingest 1g of taurine powder after each meal (3g/day in addition to their usual diets during one menstrual cycle. Before and at the end of taurine intake, physical measurements and blood collection were performed in the morning after a 12-h fast, and 3-day weighted dietary records were obtained. Concentrations of serum lipids, apolipoproteins, and fatty acids in the serum phospholipid fraction were measured. Results: The subjects showed good compliance with taurine intake and none reported adverse effects during the experimental period. After taurine intake, concentrations of total cholesterol, low density lipoprotein cholesterol (LDL-C, free cholesterol, and apolipoprotein B (apoB increased (p<0.05, while phospholipids tended to increase (p=0.051. Fatty acids in the serum phospholipid fraction also significantly increased (p<0.05. However, triglyceride, remnant-like particle cholesterol, remnant-like particle triglyceride, apoE, the apolipoprotein A-1 (apoA- 1/apoB ratio and the LDL-C/apoB ratio were unchanged. Furthermore, body weight was significantly increased (p<0.01, but did not correlate with changes either in serum lipids or nutrient intakes. Conclusion: These results suggest that high taurine intake affects lipoprotein metabolism and increases serum lipids in slightly lean young women.

  4. Alterations in serum lipid profile patterns in oral cancer

    OpenAIRE

    Singh, Simranjit; Ramesh, Venkatapathy; Premalatha, Balakrishnan; Prashad, Karthikshree Vishnu; Ramadoss, Koliyan

    2013-01-01

    Background: Alterations in serum lipids have long been associated with cancer as lipids play an important role in maintenance of cell integrity. Aims: To evaluate alterations in plasma lipid profile in oral cancer patients, to compare and correlate the serum lipid profile in different grades of carcinoma and to evaluate the correlation of serum lipid profile between the tobacco habituates and non-habituates. Materials and Methods: Among 75 study subjects, 50 individuals were oral carcinoma pa...

  5. The serum lipid peroxide level of civil aircrew members

    International Nuclear Information System (INIS)

    Objective: To observe serum lipid peroxide level and antioxidant capacity in civil aircrew members who occupationally exposed to cosmic radiation at high altitude. Methods: Serum lipid peroxides (MDA), superoxide dismutase (SOD) and total antioxidant activity (TAO) were all determined by chemical colorimetry. The effective doses were estimated in accordance with CARI-6. Results: The three indicators in the aircrew members were all significantly higher than those of ground personnel (P<0.05): their MDA, SOD, TAO levels were (4.09±1.1) nmol/ml, (126.0 ± 17.1)U/ml, (22.01 ± 4.6) U/ml, while those in the ground personnel were (3.79±0.7) nmol/ml, (119.5±10.5) U/ml, (20.02±3.9) U/ml, respectively. The serum MDA levels exhibited a trend of increase with average annual flight hours and average annual effective doses, but were not parallel with total hours service in flying and accumulative effective doses and ages. The MDA showed corresponding dynamic changes with the changes in the flight continuity over a recent period of time. Conclusions: The lipids in the body of aircrew members suffer oxidation damage from cosmic radiation and the antioxidant capacity of the body shows a compensatory increase. The serum lipid peroxide levels are affected obviously by flight burdens and the frequency exposed to cosmic radiation recently. The serum lipid peroxides might be used as a monitoring indicator in cosmic radiation protection for aircrew members

  6. STUDY OF THE CHANGES IN SERUM LIPIDS FOLLOWING MERCURY INTOXIFICATION

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    M TAHER

    2000-12-01

    Full Text Available Background. The toxicity of mercury which is used as mercury chloride in different parts of living world has been reported in literature. In our knowledge, however, these reports are on the effects of mercury on serum blood parameters. As lipids have important role in cell metabolism, we decided to study the effects of mercury on blood parameters which are related to lipid metabolism as well as its effect on hepatocyte triglycerides. Methods. Each group of 5 rats were treated with daily IP injections of either 10 mg/kg (Body Weight for 5 and 10 days or 5 mg/kg B.W. for 30 and 60 days, before determination of serum levels of triglycerides, cholesterol and lipoprotein fractions. Results. The results obtained are as follows. Daily intraperitoneal injection of 10 mg/kg B.W of mercury for 5 and 10 days elevated serum triglycerides by (10.9, 19.3 LDLc (16.5, 22.5 VLDLc (10.9, ,19.3 and hepatocytes containing triglycerides (105.1, 136.3 percent respectively. HDLc was decreased by (13.4, 17.3 percent. Daily intraperitoneal injection of 5 mg/kg body weight of mercury for 30 and 60 days elevated serum triglycerides by (34.7, 47.4. LDLc (28.9, 33.3, VLDLc (34.7, 47.4 and hepatocytes containing triglycerides (177.3, 213.4 percent respectively. HDLc was lowered by (22.9, 27.7 percent. Discussion. The results showed that mercury could affect lipid metabloism in a dose and time dependent manner. This effect might be considered as a possilbe cause of hyperlipidemia in those who are intoxified with this element.

  7. Effect of Alpha-lipoic Acid Supplementation on Serum Lipid Profile in Women with Rheumatoid Arthritis

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    Elham Mirtaheri

    2014-09-01

    Conclusions: In the present study, serum lipid profile was not significantly affected by ALA intervention. However, ALA supplementation aiming at prevention or treatment of dyslipidemia in RA patients should be further investigated. Keywords: Lipoic acid, Supplementation, Rheumatoid arthritis, Women, Lipid profile

  8. Study of serum lipids in leprosy

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    Gupta Anju

    2002-01-01

    Full Text Available Fifty fresh and untreated patients of leprosy constituted the study group. Fifty, age and sex matched healthy individuals formed the controls. Ridly and Jopling system of classification was used in the study. Majority i.e 21 cases were of BT group, 12 of BB, 7 of BL, 9 of LL and one case was of TT leprosy. The serum triglyceride level was lower than normal in TT, showed no alteration in BT or BB and was insignificantly increased in bL and LL patients. The total cholesterol was lowerthan normal in TT, showed no alteration in BT or BB and was insignificantly increased in Bland LL patients. The total cholesterol was lower than normal in TT, whereas in BT, BB, BL and LL groups the levels were statistically decreased. The HDL cholesterol was within normal range in TT, significantly decreased in BT and LL patients, showed no significant alteration in BB and was insignificantly decreased in BL group. The LDL cholesterol in TT was low but was not so low statistically when compared with the controls, whereas in BT, BB, BL and LL groups the levels were statistically decreased. The VLDL cholesterol was within normal range in TT and BT, was raised insignificantly in 3 of 12 cases of BB, was within normal range in BL and in LL leprosy it was raised in one out of 9 cases. In the absence of any derangement of liver function tests, it can be concluded that leprosy per se leads to alterations in lipid metabolism. However, no correlation could be established between the group/type of leprosy, bacterial indices and levels of different lipid fractions in the present study.

  9. Serum Lipid Profiles, Lipid Ratios and Chronic Kidney Disease in a Chinese Population

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    Liying Zhang

    2014-07-01

    Full Text Available Aim: To examine the association of serum lipids, lipid ratios with Chronic Kidney Disease (CKD in a Chinese population. Methods: Data were drawn from a cross-sectional survey in China. CKD was defined as estimated glomerular filtration rate (eGFR < 60 mL/min/1.73m2 or albuminuria-to-creatinine ratio (ACR > 30 mg/g. Multivariable logistic regressions and multivariate regression models were used. Serum lipids and lipid ratios included total cholesterol (TC, triglyceride (TG, low-density lipoprotein cholesterol (LDL-C, high-density lipoprotein cholesterol (HDL-C, TG/HDL-C ratio, TC/HDL-C ratio and LDL-C/HDL-C ratio. Results: In men, only logarithm-transformed (log TG was associated with CKD. The odds ratio (every SD increment was 1.39 (95% CI 1.03–1.87, P = 0.03. In women, none of the serum lipids and lipid ratios was associated with CKD. Using multivariate regression models, it was shown that log TG and log TG/HDL-C were negatively correlated with eGFR (P < 0.05 in men and LDL-C and log LDL-C/HDL-C ratio were correlated with ACR in men. In female subjects, serum TC, log TG, log TG/HDL-C and log TC/HDL-C were negatively correlated with eGFR (P < 0.05. All of serum lipid profiles and lipid related ratio were not correlated with ACR in women. Conclusion: Serum TG is the only suitable predictor for CKD in men. However, in women, none of serum lipids and lipid ratio can be used as a predictor for CKD. Log TG and log TG/HDL-C are negatively correlated with eGFR in both genders.

  10. Evaluation of serum and salivary lipid profile: A correlative study

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    Simranjit Singh

    2014-01-01

    Full Text Available Context: The correlation of serum and salivary lipid profile has been poorly characterized. The most commonly used laboratory diagnostic procedures for lipid profile involve analysis of cellular and chemical constituents of blood/plasma. As a diagnostic aid, saliva offers many advantages over serum. Aims: To evaluate and compare the serum and salivary lipid profile levels in healthy individuals and to validate the role of saliva as a non-invasive diagnostic tool for assessing lipid profile. Settings and Design: The present study was a prospective study. Materials and Methods: A total of 100 healthy study subjects who had no complaint or any major illness in recent past were selected. The parameters assessed included serum and salivary: total cholesterol (TC, high-density lipoprotein-cholesterol (HDLC, low-density lipoprotein-cholesterol (LDLC, very low-density lipoprotein-cholesterol (VLDLC and triglycerides (TGL. Statistical Analysis Used: Evaluation of results and statistical analysis was carried out using descriptive, correlation and regression analysis. Results: There was a moderate level of correlation between serum and salivary TC, TGL, HDLC and VLDLC and there was a low and quite small correlation between serum and salivary LDLC. For all the five parameters assessed as a part of lipid profile, the correlation coefficients were highly significant statistically and also, with an increase in the serum mean values, corresponding increase in the saliva mean values for all the five parameters was noted. Conclusions: From the present study we conclude that saliva can be used as a non-invasive diagnostic tool for assessing lipid profile.

  11. Comparison of serum lipid profile in ischaemic and haemorrhagic stroke

    International Nuclear Information System (INIS)

    To compare serum lipid profile between patients of ischaemic and haemorrhagic strokes. Study Design: Cross sectional, comparative study. Place and Duration of Study: Military Hospital, Rawalpindi, from August 2004 to February 2005. Methodology: Patients with diagnosis of stroke comprising 100 consecutive patients each of ischaemic and haemorrhagic strokes were included in the study while patients on lipid lowering therapy were excluded from study. To determine the subtype of stroke, clinical examination followed by CT scan of brain was done. A serum sample after 8 hours of overnight fasting was taken on the next day of admission for both groups of patients. Total serum cholesterol, triglycerides, LDL cholesterol, VLDL-cholesterol and HDL-cholesterol was determined, using enzymatic colorimetric method. Statistical analysis was done by comparison of lipid profile in two subgroups, using proportion test for any significant difference. Results: The mean age at presentation of patients with stroke was 64.2+-12 years with a male to female ratio of 3.6:1. In 100 ischaemic stroke patients, raised serum total cholesterol was seen in 42, triglyceride in 04, LDL-cholesterol in 05 and VLDL-cholesterol in 07 patients. Serum HDL-cholesterol was below the normal reference in 31 cases. On the other hand, serum total cholesterol and triglycerides was raised in 05 patients each, LDL-cholesterol in 09 and VLDL-cholesterol in 03 patients of haemorrhagic stroke. Serum HDL-cholesterol was below normal in 04 patients of haemorrhagic stroke. On comparison, there were significantly greater number of patients with raised serum cholesterol and low HDL-cholesterol in ischaemic stroke than haemorrhagic stroke (p < 0.05). No statistical significance was found on comparing serum values of ischaemic and haemorrhagic stroke for triglycerides, LDL-cholesterol and VLDL-cholesterol. Conclusion: Ischaemic stroke patients had high serum total cholesterol and lower HDL-cholesterol levels as compared to

  12. Correlation between Serum T3 Resin Uptake and Serum Lipid Profile

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    Dinarvand, GH. ( MSc

    2015-05-01

    Full Text Available Background and Objective: T3 resin uptake test indicates the availability of thyroxin binding globulin (TBG, which is the carrier of a large amount of T3 and T4 in blood. We aimed to study the relationship between serum T3 Resin Uptake and serum lipids profile. Material and Methods: The participants aged 14.80 ± 38.29 were both males (59; 29.5% and females (141; 70.5 %. T3 Resin Uptake was measured by Immunoassay method and serum lipid profile components (cholesterol, triglycerides, HDL-C, LDL-C, and VLDL were measured by the kit. Results: Significant negative correlation was observed between serum T3 Resin Uptake and cholesterol, triglycerides and LDL-C (P≤ 0.05, but that was not the case for HDL-C and VLDL. Conclusion: Serum T3 Resin Uptake can be evaluated to assess the risk factors related to cardiovascular disorders

  13. Cord Serum Lipid Profile of Infants of Diabetic Mothers

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    Jasim Almusawi

    2015-12-01

    Full Text Available Background: Infants of diabetic mothers (IDM is a critical issue in pediatrics, which is regarded as a major risk factor for birth trauma, respiratory distress syndrome (RDS, birth asphyxia, transient tachypnea of the newborn (TTN and jaundice. IDM is also a risk factor for microvascular (e.g., ocular and renal complications and macrovascular complications (e.g., cerebrovascular accident, atherosclerosis and cardiovascular complications. Lipids are a heterogeneous group of hydrophobic organic molecules which can be extracted from tissues using non-polar solvents. Lipids, due to their hydrophobic property, are mainly found in membranes enclosing various cell organelles. Diabetes mellitus management with insulin (nowadays also with oral hypoglycemic medications has improved the outcomes of gestational diabetes mellitus (GDM (most infants born to diabetic mother are large for gestational age. The neonatal mortality rate in IDM is over five times higher than that of infants of non-diabetic mothers. In this study, therefore, we aimed to assess the effect of maternal diabetes on cord serum lipid profile. Methods: This prospective (case-control study was carried out on 60 infants born in Al-Zahra teaching hospital during February 2014–October 2014. The study group consisted of 30 randomly chosen IDM, and the control group comprised 30 infants who were born to healthy mothers. Results: The results of this study demonstrated that there are significant differences between IDM and infants of healthy mothers regarding lipid profile and birth weight. Conclusion: This study confirms that cord serum lipid profile (serum cholesterol, serum triglycerides and low-density lipoprotein is higher at birth in IDM. Moreover, this study shows a significant association between lipid profile and body weight.

  14. Sex-Specific Associations Between Thyrotropin and Serum Lipid Profiles

    DEFF Research Database (Denmark)

    Meisinger, Christa; Ittermann, Till; Tiller, Daniel; Agger, Carsten; Nauck, Matthias; Schipf, Sabine; Wallaschofski, Henri; Jørgensen, Torben; Linneberg, Allan; Thiery, Joachim; Kluttig, Alexander; Greiser, Karin H; Werdan, Karl; Burkhardt, Katrin; Völzke, Henry

    2014-01-01

    TSH levels within the reference range (0.3-3.0 mIU/L) were significantly positively associated with triglyceride concentrations. CONCLUSIONS: Increasing levels of TSH were associated with a less favorable lipid profile in both men and women from the general population. In both sexes, TSH levels within......BACKGROUND: Population-based studies investigating the sex-specific association between thyrotropin (TSH) levels and serum lipid concentrations are scarce. We examined the association between TSH and total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL......) cholesterol, and triglycerides in men and women from the general population. Furthermore, the association with TSH outside and within the reference range and lipid levels was studied. METHODS: Individual data of 13,571 men and women without lipid medication of four population-based studies conducted in...

  15. Clinical significance of serum lipids in idiopathic pulmonary alveolar proteinosis

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    Fang Cun S

    2012-01-01

    Full Text Available Abstract Background It is well known that pulmonary alveolar proteinosis(PAP is characterised by accumulation of surfactant lipids and proteins within airspaces. However, few previous data describe the serum lipid levels associated with PAP. Materials and methods We retrospectively reviewed 25 patients with idiopathic PAP(iPAP. The serum lipid levels of patients with idiopathic PAP were compared with those of the healthy volunteers. In patients and healthy subjects, the LDL-C/HDL-C ratios were 2.94 ± 1.21 and 1.60 ± 0.70, respectively (p p A-aO2 (r = -0.685, p = 0.003, and r = 0.688, p = 0.003, respectively. The values of LDL-C/HDL-C ratios also correlated with PaO2 levels and PA-aO2 levels (r = -0.698, p = 0.003, and r = 0.653, p = 0.006, respectively. 11 and 13 patients experienced respectively a decline in TC and LDL-C levels following whole lung lavage(WLL, the median decline was 0.71 mmol/L(p p Conclusions the serum lipid levels, especially the levels of LDL-C and LDL-C/HDL-C, may reflect the severity of the disease in PAP patients, and predict the therapeutic effect of WLL.

  16. Association between chronic periodontitis and serum lipid levels

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    Pejčić Ana

    2012-01-01

    Full Text Available Background/Aim. Periodontitis is a local inflammatory process mediating destruction of periodontal tissues triggered by bacterial insult. However, this disease is also characterized by systemic inflammatory host responses that may contrbute, in part, to the recently reported increased risk for systemic diseases, including an altered lipid metabolism. On the other hand, many people in the world are affected by hyperlipidemia, which is a known risk faktor for atherosclerosis. The aim of this study was to determine the relationship between periodontal disease and blood lipid levels. Methods. A total of 50 patients with periodontitis included in this study had no documented history of recent acute coronary events. The healthy, non-periodontal subjects (comparison group comprised 25 subjects. All the patients were periodontology examined and completed a medical history. Dental plaque index, probing depth, gingival index bleeding on probing and clinical attechment levels were recorded. Blood samples were taken on admission for measurements of serum total cholesterol, triglycerides, hight density lipoprotein cholesterol (HDL-cholesterol, and low density lipoprotein cholesterol (LDL-cholesterol. Results. The obtained results showed that mean levels of cholesterol (6.09 ± 1.61 mmol/L, triglycerdes (2.19+1.67mmol/l and LDL cholesterol (4.09 ± 1.40 mmol/L in individuals with periodontitis were higer, and levels od HDL (1.43 ± 0.51 mmol/L was lower than those of individuals without periodontitis (4.86 ± 1.37; 1.14 ± 0.71; 3.18 ± 0.64; 1.53 ± 0.32 mmol/L, respectively. Conclusion. This study confirms a significant relationship between periodontal disease, regardless its intensity, and blood lipid levels in the studied population. The results imply that periodontitis may be a risk factor and may contribute to the pathogenesis of atherosclerosis and cardiovascular diseases (CVD. However, future prospective randomized studies have to determine whether

  17. Association between coffee consumption and serum lipid profile

    OpenAIRE

    KARABUDAK, EFSUN; TÜRKÖZÜ, DUYGU; KÖKSAL, EDA

    2015-01-01

    The aim of the present study was to investigate the association between coffee consumption and serum lipid levels in a study population of 122 Turkish subjects (mean age, 41.4±12.69 years), including 48 males and 74 females. A questionnaire was compiled to determine baseline characteristics, and food and coffee consumption. Subjects were divided into three groups, which included non-drinkers, Turkish coffee and instant coffee drinkers, and anthropometric measurements were acquired, including ...

  18. Epidemiologic evidence on serum adiponectin level and lipid profile

    OpenAIRE

    Vajihe Izadi; Elaheh Farabad; Leila Azadbakht

    2013-01-01

    The concentration of adiponectin, a hormone which is secreted from adipose tissue, is inversely correlated with body fat mass. This hormone has anti inflammatory and anti atherogenic properties. Its concentration reduces in metabolic syndrome and cardiovascular diseases. This study reviews the evidence on the relationship between serum adiponectin concentration and lipid profile. In this study former clinical trials, cross sectional and prospective studies have been reviewed. The PubMed searc...

  19. Serum Calcium Increase Correlates With Worsening of Lipid Profile

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    Gallo, Luigia; Faniello, Maria C.; Canino, Giovanni; Tripolino, Cesare; Gnasso, Agostino; Cuda, Giovanni; Costanzo, Francesco S.; Irace, Concetta

    2016-01-01

    Abstract Despite the well-documented role of calcium in cell metabolism, its role in the development of cardiovascular disease is still under heavy debate. Several studies suggest that calcium supplementation might be associated with an increased risk of coronary heart disease, whereas others underline a significant effect on lowering high blood pressure and hyperlipidemia. The purpose of this study was to investigate, in a large nonselected cohort from South Italy, if serum calcium levels correlate with lipid values and can therefore be linked to higher individual cardiovascular risk. Eight-thousand-six-hundred-ten outpatients addressed to the Laboratory of Clinical Biochemistry, University of Magna Græcia, Catanzaro, Italy from January 2012 to December 2013 for routine blood tests, were enrolled in the study. Total HDL-, LDL- and non-HDL colesterol, triglycerides, and calcium were determined with standard methods. We observed a significant association between total cholesterol, LDL-cholesterol, HDL-cholesterol, non-HDL cholesterol, triglycerides, and serum calcium in men and postmenopause women. Interestingly, in premenopause women, we only found a direct correlation between serum calcium, total cholesterol, and HDL-cholesterol. Calcium significantly increased while increasing total cholesterol and triglycerides in men and postmenopause women. Our results confirm that progressive increase of serum calcium level correlates with worsening of lipid profile in our study population. Therefore, we suggest that a greater caution should be used in calcium supplement prescription particularly in men and women undergoing menopause, in which an increase of serum lipids is already known to be associated with a higher cardiovascular risk. PMID:26937904

  20. LC/MS lipid profiling from human serum: a new method for global lipid extraction.

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    Pellegrino, Roberto Maria; Di Veroli, Alessandra; Valeri, Aurora; Goracci, Laura; Cruciani, Gabriele

    2014-12-01

    Over the last decade, technological advances have improved the sensitivity and selectivity of LC/MS analyzers, providing very efficient tools for lipidomics research. In particular, the nine lipid classes that constitute 99 % of the human serum lipidome (sterols, cholesteryl esters, phosphocholines, phosphoethanolamines, sphingomyelins, triacylglycerols, fatty acids, lysophosphocholines, and diacylglycerols) can be easily detected. However, until today there has not been a unique technique for sample preparation that provides a satisfactory recovery for all of these nine classes together. In this work, we have developed and validated a new one-phase extraction (OPE) method that overcomes this limitation. This method was also compared with the gold standard lipid extraction methods such as Folch, Bligh & Dyer, and recently developed methods with methanol and methyl-tert-butyl ether. Results demonstrate that the mixture of methanol/chloroform/MTBE (MMC) provides a recovery very close to 100 % for all nine lipid classes of the human serum investigated. For this extraction method, 100 μL of human serum is incubated with 2 mL of the solvents mixture, then vortexed and centrifuged. For its simplicity of execution, rapidity, reproducibility, and the reduced volume of sample required, this method opens the door to the use of human serum lipid profiling for large-scale applications in scientific research and clinical trials. PMID:25381612

  1. Effects of consumption of probiotics and prebiotics on serum lipid levels in humans.

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    Pereira, Dora I A; Gibson, Glenn R

    2002-01-01

    The objective of this article is to review existing studies concerning the effects of probiotics and prebiotics on serum cholesterol concentrations, with particular attention on the possible mechanisms of their action. Although not without exception, results from animal and human studies suggest a moderate cholesterol-lowering action of dairy products fermented with appropriate strain(s) of lactic acid bacteria and bifidobacteria. Mechanistically, probiotic bacteria ferment food-derived indigestible carbohydrates to produce short-chain fatty acids in the gut, which can then cause a decrease in the systemic levels of blood lipids by inhibiting hepatic cholesterol synthesis and/or redistributing cholesterol from plasma to the liver. Furthermore, some bacteria may interfere with cholesterol absorption from the gut by deconjugating bile salts and therefore affecting the metabolism of cholesterol, or by directly assimilating cholesterol. For prebiotic substances, the majority of studies have been done with the fructooligosaccharides inulin and oligofructose, and although convincing lipid-lowering effects have been observed in animals, high dose levels had to be used. Reports in humans are few in number. In studies conducted in normal-lipidemic subjects, two reported no effect of inulin or oligofructose on serum lipids, whereas two others reported a significant reduction in serum triglycerides (19 and 27%, respectively) with more modest changes in serum total and LDL cholesterol. At present, data suggest that in hyperlipidemic subjects, any effects that do occur result primarily in reductions in cholesterol, whereas in normal lipidemic subjects, effects on serum triglycerides are the dominant feature. PMID:12236466

  2. Ectopic and Serum Lipid Levels Are Positively Associated with Bone Marrow Fat in Obesity

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    Bredella, Miriam A.; Gill, Corey M.; Gerweck, Anu V.; Landa, Melissa G.; Kumar, Vidhya; Daley, Scott M.; Torriani, Martin; Miller, Karen K.

    2013-01-01

    Mean ectopic lipid levels, such as intrahepatic lipid and intramyocellular lipid levels, and serum lipid levels are significantly positively associated with bone marrow fat in young obese men and women; because bone marrow fat is known to be inversely related to bone mineral density, these results support the notion that ectopic and serum lipid levels are influenced by the same additional factors as bone marrow or may exert negative effects on bone.

  3. Relationship Between Exposure to Industrial Noise and Serum Lipid Profile

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    Ramin Mehrdad

    2011-11-01

    Full Text Available Aim of our study was to investigate the effects of exposure to industrial noise on serum lipid profile among workers who are exposed to noise at work. In a historical cohort study, we recruited 154 and 146 male workers as high and low level noise exposure groups respectively. We defined workers with at least one year exposure to noise level more than 90 dB as high exposure group, and those with exposure to less than 80 dB as low exposure group. Afterwards, in the fasting blood specimens of participants we measured serum Triglyceride (TG, total Cholesterol (TC, high and low density lipoprotein (HDL and LDL. Mean of TG, TC, HDL and LDL for low exposure group were 148, 189, 38 and 103 mg/dl and for high exposure group were 237, 189, 37 and 104 mg/dl respectively. Mean serum TG between two groups was different. Even after adjustment for age, BMI, smoking and work hours per week, serum TG among high exposure group was 89 mg/dl higher than low exposure group and this difference was statistically significant (P=0.00. There was no significant difference between two groups in TC, LDL and HDL levels. This study did not find a statistically significant relationship between exposure to noise and serum TC, LDL and HDL, but TG in two groups was different and this difference was statistically significant.

  4. Evaluation the Effect Garlet Tablet on Serum Lipid Profile

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    F. Emami

    2006-07-01

    Full Text Available Introduction & Objective: Some investigators reported significant effect of garlic on serum cholesterol reduction. In addition, Iranian culture has specific belief on herbs and garlic in this regard. Our goal in this study was to evaluate the effect of garlet tablets on serum lipid profile.Materials & Methods: Sixty patients were randomly divided into two groups for evaluation of the effect of garlic on their lipid profile. The first group was low fat regimen group and the second was garlet tablet regimen group. Total cholesterol, LDL cholesterol, HDL cholesterol and triglyceride levels were measured in both groups. Then, after two months of these regimens administration, these items were measured again and were compared.Results: Mean age, sex and baseline initial lipid levels were similar in both groups. Total cholesterol and LDL cholesterol levels were decreased significantly in the garlic regimen group (in spite of non significant reduction in the other group. Triglyceride and HDL levels were not changed significantly in both regimen groups. Conclusion: Garlet tablet administration has more significant reductive effect on cholestrol level than low cholesterol diet.

  5. Depressive symptoms and serum lipid levels in young adult women.

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    Fang, Carolyn Y; Egleston, Brian L; Gabriel, Kelley Pettee; Stevens, Victor J; Kwiterovich, Peter O; Snetselaar, Linda G; Longacre, Margaret L; Dorgan, Joanne F

    2013-04-01

    Accumulating data suggest that depression is associated with risk factors for cardiovascular disease, but few studies have investigated potential behavioral mediators of such associations, particularly among women. In this study of healthy young adult women (n = 225), we examined associations among depressive symptoms, health behaviors, and serum lipid levels. Depressive symptoms were assessed with the 20-item Center for Epidemiologic Studies-Depression scale, and a fasting blood sample was obtained for serum lipid levels, including total cholesterol, high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C). Diet was measured using 24-h recalls, and other health behaviors (physical activity, smoking) were assessed via self-report questionnaire. Results indicated a modest negative association between depressive symptoms and LDL-C levels. Higher levels of depressive symptoms were also associated with lower total and insoluble dietary fiber intake, both of which were associated with HDL-C and LDL-C. Mediational analyses indicated a significant indirect effect of depressive symptoms on LDL-C via total and insoluble dietary fiber in unadjusted analyses, but not in adjusted analyses. The present findings suggest that depressive symptoms are inversely associated with serum LDL-C levels in young adult women, but that these associations are not likely mediated by adverse lifestyle behaviors. PMID:22382824

  6. Increased serum potassium affects renal outcomes

    DEFF Research Database (Denmark)

    Miao, Y; Dobre, D; Heerspink, H J Lambers; Brenner, B M; Cooper, M E; Parving, Hans-Henrik Dyring; Shahinfar, S; Grobbee, D; de Zeeuw, D

    2011-01-01

    To assess the effect of an angiotensin receptor blocker (ARB) on serum potassium and the effect of a serum potassium change on renal outcomes in patients with type 2 diabetes and nephropathy.......To assess the effect of an angiotensin receptor blocker (ARB) on serum potassium and the effect of a serum potassium change on renal outcomes in patients with type 2 diabetes and nephropathy....

  7. Serum lipid & lipoprotein profiles of obese Chinese children.

    Science.gov (United States)

    Ho, T F; Paramsothy, S; Aw, T C; Yip, W C

    1996-03-01

    The serum lipid and lipoprotein levels of 59 obese Chinese children with a mean age of 13.0 years and mean relative weight of 164.2% were analysed. Between 40% to 54% of these children had elevated lipid and lipoprotein levels and about 78% had reduced high density lipoprotein (HDL) level when compared to healthy American and Japanese children. The obese children also had higher mean levels of total cholesterol (TC) and lower HDL compared to male adults in the local population. Those with elevated TC had higher mean relative weight (170% vs 159%, p obese children should be carefully screened and managed to prevent long term morbidity and mortality of coronary artery disease. PMID:10967982

  8. Serum Iron, Haemoglobin and Serum Lipid Peroxidation in Neonates with Respiratory Disorders

    Directory of Open Access Journals (Sweden)

    Sushama P. Dhonde

    2010-01-01

    Full Text Available Respiratory disorders are the most common cause for higher morbidity and mortality rate in India. Higher oxygen concentration of extrauterine existence causes increased erythrocyte lysis lead to release of iron in neonates. Iron is known to catalyze the formation of Reactive oxygen species. Involvement of hemoglobin and iron in oxygen-mediated reactions stimulate us to study the role of these in neonates.Objectives :iTo estimate the concentration of Haemoglobin, serum iron, serum lipid peroxidation in neonates with respiratory disorders; and compare those with that of healthy controls. iiTo study the role of these parameters in neonates with respiratory disorders.Materials and Methods: Present study includes 50 neonates suffering from respiratory disorders and 50 healthy neonates as controls. Samples collected from these were used for the estimation of haemoglobin, serum iron, and serum lipid peroxidation.Observations: Significantly (p<0.001 increased levels of serum iron and lipid peroxidation were observed in neonates with respiratory disorders when compared those with control values. These levels were found significantly (p<0.001 higher in preterm than full-term neonates. Concentration of haemoglobin showed no significant difference in both groups.Conclusion: Exacerbation of oxidative stress in neonates with respiratory disorders may be due to hypoxia induced free radical generation, higher oxidative tendency of HbF and elevated iron. Premature neonates are probably unprepared for extra uterine life in an oxygen rich environment and due to this they are more prone to oxidative insult. Thus this study reveals the pro-oxidant role of HbF and iron, which enhances the oxidative stress in respiratory disorder.

  9. Effect of Consumption of Tribulus Terrestris on Serum Glucose and Lipid Levels in Diabetic Rats

    Directory of Open Access Journals (Sweden)

    M Roghani

    2010-03-01

    Full Text Available Introduction: The effect of Tribulus terrestris (TT on serum glucose and lipid levels was investigated in an experimental model of diabetes mellitus in rats. Methods: Female Wistar rats were divided into control, TT-treated control, diabetic, glibenclamide-treated, and TT-treated diabetic groups. For induction of diabetes, streptozotcin (STZ was administered (60 mg/Kg. Meanwhile, TT-treated groups received TT mixed with standard pelleted food at a weight ratio of 6.25% for 6 weeks. Serum glucose and lipid levels were determined before the study and at the 3rd and 4th week after the study. Results: Serum glucose was significantly lower in TT-treated diabetic rats at 3rd and 6th weeks as compared to untreated diabetics (p<0.01 and p<0.005, respectively. In addition, serum total cholesterol, triglyceride, and LDL-cholesterol showed a significant reduction in TT-treated diabetic rats as compared to untreated diabetics (p<0.05. On the other hand, HDL-cholesterol level did not change significantly in TT-treated diabetic group as compared to untreated diabetic group. Conclusions: Oral administration of TT has a significant hypoglycemic effect and in long term leads to appropriate changes in serum LDL-cholesterol, total cholesterol, and triglyceride levels, but does not affect HDL-cholesterol levels in diabetic rats.

  10. Association between different degrees of hypothyroidism and serum lipids

    Directory of Open Access Journals (Sweden)

    Vandana Saini

    2012-07-01

    Full Text Available The association between overt hypothyroidism (OH and altered lipid profile is well known, however the significance of dyslipidemia in subclinical hypothyroidism (SCH remain controversial. Therefore, this study was conducted to determine any association between lipid profile and different degrees of thyroid dysfunction. Thyroid and lipid profile parameters were analysed in 58 patients with overt (TSH ≥ 10.0 µIU/L and/or abnormally low fT4 and fT3 levels and 87 patients with subclinical hypothyroidism (TSH 6.0-9.9 µIU/L with normal fT4 and fT3 levels in this case-control study. These were compared with 100 age- and sex-matched euthyroid controls. It was found that only mean serum level of total cholesterol in patients with SCH was significantly high from that in controls (p=0.045. Other lipid parameters did not show any statistical significance. Whereas patients with OH had statistically significant higher levels of total cholesterol (p<0.001, triglyceride (p<0.05, LDL-C (p<0.001 and VLDL-C (p<0.05. There was also an increase in HDL-C in both SCH and OH group though not significant statistically. In conclusion, lipid profile is not much deranged in SCH whereas OH is a major cause of secondary dyslipidemia which may lead to increased risk of coronary artery disease. Therefore, thyroid hormone replacement would be most beneficial in patients with OH instead of SCH. However, patients with SCH should be monitored for deterioration of thyroid function and dyslipidemia at regular intervals.

  11. Serum glucose concentration and lipid profile in racing horses

    Directory of Open Access Journals (Sweden)

    S. A. Hasso

    2012-01-01

    Full Text Available The aim of the present work was to evaluate serum glucose concentration and lipid profile in racing horses in Iraq. Blood samples were collected from the jugular vein of 92 clinically healthy racing horses (males and females, Arabian and Cross bred, 2-8 years old at Equestrian Club, Baghdad. Investigations included serum measurements of glucose (sg and lipid profile parameters; total cholesterol (TC, Triglyceride (TG, high density lipoprotein- cholesterol (HDL-C, low density lipoprotein-cholesterol (LDL-C, very low density lipoprotein-cholesterol (VLDL-C and the atherogenic ratio (LDL-C/HDL-C, in order to register the normal ranges and mean values of these measured parameters in Iraqi healthy racing horses. Results showed that the range and mean values ± standard error of sg were: 33.3 – 6.71 mmol/l and 5.17±0.07 mmol/l, respectively, whereas the TC was 2.07 – 4.22 mmol/l and 3.01±0.05 mmol/l, TG 0.6 – 1.47 mmol/l and 1.06±0.02 mmol/l, HDL-C 0.93 – 2.25 mmol/l and 1.50±0.03 mmol/l, LDL-C 0.10 – 2.12 mmol/l and 0.91±0.04 mmol/l, VLDL-C 0.31 -0.67 mmol/l and 0.55±0.02 mmol/l, respectively and the atherogenic ratio 0.66±0.03. The data present reference values and mean ± SE for sg and lipid profile parameters in healthy racing horses in Baghdad.

  12. Impact of age, gender and diabetes on serum lipid levels

    International Nuclear Information System (INIS)

    Objectives:To see the effects of age, gender and diabetes on serum lipid levels. Study type, settings and duration:Cross sectional analytical study conducted at PMRC Research Centre, Fatima Jinnah Medical College,Lahore from Jun-Dec 2011. Materials and Methods: One hundred type 2 diabetes mellitus and equal numbers of gender matched healthy controls were randomly selected for the study. After an overnight fasting, blood specimens were drawn for lipid profile where total cholesterol,high density lipoprotein cholesterol and triglycerides were measured by standard enzymatic endpoint methods and LDL-cholesterol by Friedweld's formula. Statistical analysis was done using SPSS-15. Results Odd ratios of age for total cholesterol (OR 1.198); high density lipoprotein cholesterol (OR 1.144); and low density lipoprotein cholesterol (OR 1.576) revealed that subjects aged 45 years or more had greater risk of having deranged lipid levels. Female gender had higher high density lipoprotein cholesterol (p 0.000); and triglycerides (p 0.001). Odd ratios of men for total cholesterol (OR 0.775); high density lipoprotein cholesterol(OR 0.183); and low density lipoprotein Cholesterol (OR 0.683) illustrated that men were significantly less prone to dyslipidemia than women. Odd ratio of diabetes for high density lipoprotein cholesterol (OR 1.802) suggested that diabetics had 1.8 times more risk of having low high density lipoprotein cholesterol. Conclusions: Diabetic females over 45 years of age have significantly high chances of having disturbed or high lipid profile. Policy message:All persons in general and diabetics in particular over the age of 45 years should be screened for dyslipidemia and informed using behavior change communication to prevent disease complications. (author)

  13. Effect of cobalt chloride on content of lipids and lipoproteins in serum and liver of rats.

    Science.gov (United States)

    Kaliman, P A; Shalamov, R V; Zagaiko, A L

    1997-07-01

    Lipids and the composition of lipoproteins in blood serum and liver cytosol, total lipid, and phospholipid contents in liver subcellular fractions and the spectrum of microsomal liver lipids were studied in male Wistar rats after a single injection of cobalt chloride. Virtually all lipid and lipoprotein fractions in blood and liver were increased and lipoprotein composition was changes. The lipid composition of liver microsomes did not change under these conditions. Thus, microsomal membranes are stable under developing oxidative stress. PMID:9331964

  14. Prognostic Implications of Serum Lipid Metabolism over Time during Sepsis

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    Sang Hoon Lee

    2015-01-01

    Full Text Available Background. Despite extensive research and an improved standard of care, sepsis remains a disorder with a high mortality rate. Sepsis is accompanied by severe metabolic alterations. Methods. We evaluated 117 patients with sepsis (severe sepsis [n=19] and septic shock [n=98] who were admitted to the intensive care unit. Serum cholesterol, triglyceride (TG, high-density lipoprotein (HDL, low-density lipoprotein (LDL, free fatty acid (FFA, and apolipoprotein (Apo A-I levels were measured on days 0, 1, 3, and 7. Results. Nonsurvivors had low levels of cholesterol, TG, HDL, LDL, and Apo A-I on days 0, 1, 3, and 7. In a linear mixed model analysis, the variations in TG, LDL, FFA, and Apo A-I levels over time differed significantly between the groups (p=0.043, p=0.020, p=0.005, and p=0.015, resp.. According to multivariate analysis, TG levels and SOFA scores were associated with mortality on days 0 and 1 (p=0.018 and p=0.008, resp.. Conclusions. Our study illustrated that TG levels are associated with mortality in patients with sepsis. This may be attributable to alterations in serum lipid metabolism during sepsis, thus modulating the host response to inflammation in critically ill patients.

  15. Serum lipid profile and its association with hypertension in Bangladesh

    Directory of Open Access Journals (Sweden)

    Choudhury KN

    2014-06-01

    Full Text Available Kamrun Nahar Choudhury,1 AKM Mainuddin,2 Mohammad Wahiduzzaman,3 Sheikh Mohammed Shariful Islam4,51Department of Epidemiology, National Centre for Control of Rheumatic Fever and Heart Disease, 2Center for Communicable Diseases, International Center for Diarrheal Disease Research, 3Department of Cardiology, Bangladesh Institute of Health Science, Bangladesh, Dhaka, Bangladesh; 4Center for Control of Chronic Diseases, International Center for Diarrheal Disease Research, Bangladesh, Dhaka, Bangladesh; 5Center for International Health, University of Munich, Munich, GermanyBackground: Hypertension and dyslipidemia are major risk factors for cardiovascular disease, accounting for the highest morbidity and mortality among the Bangladeshi population. The objective of this study was to determine the association between serum lipid profiles in hypertensive patients with normotensive control subjects in Bangladesh.Methods: A cross-sectional study was carried out among 234 participants including 159 hypertensive patients and 75 normotensive controls from January to December 2012 in the National Centre for Control of Rheumatic Fever and Heart Disease in Dhaka, Bangladesh. Data were collected on sociodemographic factors, anthropometric measurements, blood pressure, and lipid profile including total cholesterol (TC, triglyceride (TG, low density lipoprotein (LDL, and high density lipoprotein (HDL.Results: The mean (± standard deviation systolic blood pressure and diastolic blood pressure of the participants were 137.94±9.58 and 94.42±8.81, respectively, which were higher in the hypertensive patients (P<0.001. The serum levels of TC, TG, and LDL were higher while HDL levels were lower in hypertensive subjects compared to normotensives, which was statistically significant (P<0.001. Age, waist circumference, and body mass index showed significant association with hypertensive patients (P<0.001 but not with normotensives. The logistic regression analysis showed

  16. Spastin binds to lipid droplets and affects lipid metabolism.

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    Chrisovalantis Papadopoulos

    2015-04-01

    Full Text Available Mutations in SPAST, encoding spastin, are the most common cause of autosomal dominant hereditary spastic paraplegia (HSP. HSP is characterized by weakness and spasticity of the lower limbs, owing to progressive retrograde degeneration of the long corticospinal axons. Spastin is a conserved microtubule (MT-severing protein, involved in processes requiring rearrangement of the cytoskeleton in concert to membrane remodeling, such as neurite branching, axonal growth, midbody abscission, and endosome tubulation. Two isoforms of spastin are synthesized from alternative initiation codons (M1 and M87. We now show that spastin-M1 can sort from the endoplasmic reticulum (ER to pre- and mature lipid droplets (LDs. A hydrophobic motif comprised of amino acids 57 through 86 of spastin was sufficient to direct a reporter protein to LDs, while mutation of arginine 65 to glycine abolished LD targeting. Increased levels of spastin-M1 expression reduced the number but increased the size of LDs. Expression of a mutant unable to bind and sever MTs caused clustering of LDs. Consistent with these findings, ubiquitous overexpression of Dspastin in Drosophila led to bigger and less numerous LDs in the fat bodies and increased triacylglycerol levels. In contrast, Dspastin overexpression increased LD number when expressed specifically in skeletal muscles or nerves. Downregulation of Dspastin and expression of a dominant-negative variant decreased LD number in Drosophila nerves, skeletal muscle and fat bodies, and reduced triacylglycerol levels in the larvae. Moreover, we found reduced amount of fat stores in intestinal cells of worms in which the spas-1 homologue was either depleted by RNA interference or deleted. Taken together, our data uncovers an evolutionarily conserved role of spastin as a positive regulator of LD metabolism and open up the possibility that dysfunction of LDs in axons may contribute to the pathogenesis of HSP.

  17. Links between cardiovascular disease and osteoporosis in postmenopausal women: serum lipids or atherosclerosis per se?

    DEFF Research Database (Denmark)

    Bagger, Y Z; Rasmussen, Henrik Berg; Alexandersen, P; Werge, T; Christiansen, C; Tankó, L B

    2007-01-01

    Epidemiological observations suggest links between osteoporosis and risk of acute cardiovascular events and vice versa. Whether the two clinical conditions are linked by common pathogenic factors or atherosclerosis per se remains incompletely understood. We investigated whether serum lipids and p...... polymorphism in the ApoE gene modifying serum lipids could be a biological linkage.......Epidemiological observations suggest links between osteoporosis and risk of acute cardiovascular events and vice versa. Whether the two clinical conditions are linked by common pathogenic factors or atherosclerosis per se remains incompletely understood. We investigated whether serum lipids and...

  18. Modifications in dietary fat quality are associated with changes in serum lipids of older adults independently of lipid medication.

    Science.gov (United States)

    Buyken, Anette E; Flood, Victoria; Rochtchina, Elena; Nestel, Paul; Brand-Miller, Jennie; Mitchell, Paul

    2010-01-01

    Prospective evidence on the extent to which serum lipid concentrations in older persons respond to dietary modification is scarce. It is not clear whether such behavioral changes are relevant in the context of more commonly initiated treatments with lipid-lowering drugs. We therefore examined whether individual changes in the consumption of dietary fatty acids or main food sources were associated with changes in the serum lipid profile of older Australians. A total of 903 participants (> or =49 y) in the Blue Mountains Eye Study had complete data on fasting lipids and dietary intake from a validated FFQ at baseline (1992-1994) and 5- and 10-y follow-up examinations. Decreasing consumption of SFA and butter during the 10-y period were associated with moderate decreases in serum total cholesterol independently of initiation of lipid-lowering drug treatment [adjusted estimates were 0.018 +/- 0.007 mmol/(L x % energy (%en) from SFA (P = 0.01) and 0.055 +/- 0.015 mmol/(L x 5 g butter) (P = 0.0003), respectively]. Increased consumption of (n-3) fatty acids and fish was independently related to modest increases in serum HDL-cholesterol [0.067 +/- 0.026 mmol/(L x %en from (n-3) fatty acids) (P = 0.01) and 0.010 +/- 0.004 mmol/(L x 20 g fish) (P = 0.02)] and decreases in log-transformed serum triglyceride concentrations [P = 0.02 for (n-3) fatty acids and P = 0.02 for fish intake]. Hence, 10-y changes in the intake of dietary fatty acids and their food sources appear to have contributed to concurrent improvements in the serum lipid profile of older Australians, independent of concomitantly initiated lipid-lowering drug treatment. PMID:19923396

  19. Prenatal hyperandrogenism induces alterations that affect liver lipid metabolism.

    Science.gov (United States)

    Abruzzese, Giselle Adriana; Heber, Maria Florencia; Ferreira, Silvana Rocio; Velez, Leandro Martin; Reynoso, Roxana; Pignataro, Omar Pedro; Motta, Alicia Beatriz

    2016-07-01

    Prenatal hyperandrogenism is hypothesized as one of the main factors contributing to the development of polycystic ovary syndrome (PCOS). PCOS patients have high risk of developing fatty liver and steatosis. This study aimed to evaluate the role of prenatal hyperandrogenism in liver lipid metabolism and fatty liver development. Pregnant rats were hyperandrogenized with testosterone. At pubertal age, the prenatally hyperandrogenized (PH) female offspring displayed both ovulatory (PHov) and anovulatory (PHanov) phenotypes that mimic human PCOS features. We evaluated hepatic transferases, liver lipid content, the balance between lipogenesis and fatty acid oxidation pathway, oxidant/antioxidant balance and proinflammatory status. We also evaluated the general metabolic status through growth rate curve, basal glucose and insulin levels, glucose tolerance test, HOMA-IR index and serum lipid profile. Although neither PH group showed signs of liver lipid content, the lipogenesis and fatty oxidation pathways were altered. The PH groups also showed impaired oxidant/antioxidant balance, a decrease in the proinflammatory pathway (measured by prostaglandin E2 and cyclooxygenase-2 levels), decreased glucose tolerance, imbalance of circulating lipids and increased risk of metabolic syndrome. We conclude that prenatal hyperandrogenism generates both PHov and PHanov phenotypes with signs of liver alterations, imbalance in lipid metabolism and increased risk of developing metabolic syndrome. The anovulatory phenotype showed more alterations in liver lipogenesis and a more impaired balance of insulin and glucose metabolism, being more susceptible to the development of steatosis. PMID:27179108

  20. Serum Lipid Levels in Patients with Eating Disorders.

    Science.gov (United States)

    Nakai, Yoshikatsu; Noma, Shun'ichi; Fukusima, Mitsuo; Taniguchi, Ataru; Teramukai, Satoshi

    2016-01-01

    Objective To evaluate some risk factors for cardiovascular diseases in feeding and eating disorders, the degree of lipid abnormalities was investigated in a large Japanese cohort of different groups of feeding and eating disorders, according to the Japan Atherosclerosis Society Guidelines for the Prevention of Atherosclerotic Cardiovascular Diseases 2012 (JAS Guidelines 2012). Methods Participants in the current study included 732 women divided into four groups of feeding and eating disorders: anorexia nervosa, restricting type (AN-R); anorexia nervosa, binge-eating/purging type; bulimia nervosa (BN); and binge-eating disorder (BED). We measured the serum levels of total cholesterol, high-density-lipoprotein (HDL) cholesterol, and triglyceride in these participants. Low-density-lipoprotein (LDL) cholesterol and non-HDL cholesterol levels were also calculated. Results The concentrations of LDL cholesterol and non-HDL cholesterol were widely distributed in all groups. When the LDL cholesterol risk was defined as ≥120 mg/dL and the non-HDL cholesterol risk as ≥150 mg/dL, according to the JAS Guidelines 2012, the proportion of LDL cholesterol risk ranged from 29.6% (BN) to 38.6% (AN-R), and the proportion of non-HDL cholesterol risk ranged from 17.8% (BN) to 30.1% (BED). Conclusion The present findings suggest the existence of LDL cholesterol risk and non-HDL cholesterol risk in all groups of eating disorders. Given the chronicity of this condition, the development of elevated concentrations of LDL cholesterol and non-HDL cholesterol at an early age may increase the risk of cardiovascular diseases. PMID:27432092

  1. Serum lipid profile and uric acid levels in preeclampsia in University of Benin Teaching Hospital

    OpenAIRE

    Enaruna, Nosakhare O; Joseph O Idemudia; Aikoriogie, Paul I

    2014-01-01

    Background: Preeclampsia is a pregnancy-specific disease associated with significant maternal and perinatal mortality and morbidity. Lipid abnormality and elevated serum uric acid have been reported as early features of the disease. We aimed to detect the level of serum lipid profile and uric acid abnormalities in severe preeclamptics in Benin City and to measure their clinical significance. Materials and Methods: A prospective case-control study was conducted with subjects presenting with se...

  2. Effects of Krill Oil on serum lipids of hyperlipidemic rats and human SW480 cells

    OpenAIRE

    Qian Wen-Bin; Shi Jia-Hui; Zhu Jia-Jin; Cai Zhen-Zhen; Li Duo

    2008-01-01

    Abstract Background Cardiovascular disease (CVD) and colon cancer incidence are known to be closely related to dietary factors. This article evaluated effects of krill oil (KO) on serum lipids of hyperlipidemia rats and human colon cancer cells (SW480). Serum lipids of rats fed with high fat diet (HFD) and different doses of KO were measured by automatic analyzer. Effect of KO on viability of cells was determined by methyl thiazolyl tetrazolium (MTT) assay. Results Except for higher dose grou...

  3. The Effect of Hibiscus Sabdariffa on Lipid Profile, Creatinine, and Serum Electrolytes: A Randomized Clinical Trial

    OpenAIRE

    Abbas Mohagheghi; Shirin Maghsoud; Patricia Khashayar; Mohammad Ghazi-Khansari

    2010-01-01

    Background. Hibiscus Sabdariffa L. (HS), a member of malvaceae family, is a medicinal plant with a worldwide fame. Its effect on reducing serum lipids is mentioned in several studies. The purpose of this study was to assess the efficacy of this plant in reducing the serum's lipids in hypertensive patients. Materials and Methods. Ninety hypertensive patients were randomly assigned to receive Hibiscus Sabdariffa (HS) tea or black tea for 15 days. The patients were asked to drink the tea within ...

  4. Determination of serum aluminum, platelet aggregation and lipid peroxidation in hemodialyzed patients

    OpenAIRE

    T.J.C. Neiva; A.L. Benedetti; S.M.C.N. Tanaka; Santos, J. I.; E.A. D'Amico

    2002-01-01

    Aluminum (Al3+) overload is frequently associated with lipid peroxidation and neurological disorders. Aluminum accumulation is also reported to be related to renal impairment, anemia and other clinical complications in hemodialysis patients. The aim of the present study was to determine the degree of lipid peroxidation, platelet aggregation and serum aluminum in patients receiving regular hemodialytic treatment. The level of plasma lipid peroxidation was evaluated on the basis of thiobarbitur...

  5. Serum lipid and apolipoprotein distributions in Hong Kong Chinese.

    OpenAIRE

    Fong, P C; Tam, S C; Tai, Y. T.; Lau, C P; Lee, J.; Sha, Y Y

    1994-01-01

    STUDY OBJECTIVE--The aim was to describe the distribution of lipids and apolipoproteins in the Chinese population in Hong Kong. DESIGN--This was a prospective, cross sectional, population based survey. SETTINGS--The study was conducted in a single, self referred, out patient screening centre. PARTICIPANTS--Altogether 825 Chinese adults aged > or = 20 years were screened. One hundred subjects who had previously had lipid measurement and 29 who were taking lipid modifying drugs were excluded bu...

  6. Resistin gene polymorphisms are associated with acne and serum lipid levels, providing a potential nexus between lipid metabolism and inflammation.

    Science.gov (United States)

    Younis, Sidra; Blumenberg, Miroslav; Javed, Qamar

    2016-05-01

    Acne vu lgaris is a multifactorial inflammatory skin disease causing social stigma and psychological effect on patients. We hypothesized that the genes that can affect both lipid metabolism and inflammation may be central for acne formation and present targets for treatment. Pro-inflammatory adipokine resistin, one such likely target, activates NFkB and JNK pathways inducing TLR-2, IL-1, IL-6, and TNFα genes. The polymorphisms in promoter and intron region of the resistin gene affect its expression levels. Therefore, we explored the association of resistin polymorphisms (RETN +299G > A and -420C > G) with pathogenesis of acne vulgaris. We used PCR-RFLP method to genotype at the two single nucleotide polymorphisms at RETN promoter in 530 acne patients vs. 550 age- and sex-matched control subjects. We also measured serum lipid levels in acne patients and associated these with RETN genotypes. We found that the RETN gene polymorphisms are strongly associated with acne vulgaris and the severity of acne symptoms. In females the variant allele frequencies of both SNPs are statistically higher in patients than in controls; in males frequency distribution does not reach significance. The haplotype containing both variant alleles is significantly more common in patients than in controls. We find no association of RETN SNPs with the acne types. Importantly, we found that the levels of HDL-C were significantly decreased in variant genotype of RETN. Our results show that the RETN polymorphisms expected to boost resistin expression increase the risk of developing acne. We suggest that resistin may provide an attractive target for treatment. PMID:26858108

  7. Mechanics of lipid bilayer junctions affecting the size of a connecting lipid nanotube

    OpenAIRE

    Voinova Marina; Kurczy Michael; Cans Ann-Sofie; Adams Kelly; Grzhibovskis Richards; Karlsson Roger; Ewing Andrew

    2011-01-01

    Abstract In this study we report a physical analysis of the membrane mechanics affecting the size of the highly curved region of a lipid nanotube (LNT) that is either connected between a lipid bilayer vesicle and the tip of a glass microinjection pipette (tube-only) or between a lipid bilayer vesicle and a vesicle that is attached to the tip of a glass microinjection pipette (two-vesicle). For the tube-only configuration (TOC), a micropipette is used to pull a LNT into the interior of a surfa...

  8. Effect of honey on serum cholesterol and lipid values.

    Science.gov (United States)

    Münstedt, Karsten; Hoffmann, Sven; Hauenschild, Annette; Bülte, Michael; von Georgi, Richard; Hackethal, Andreas

    2009-06-01

    Small studies have suggested that honey benefits patients with high cholesterol concentrations. The present study aimed to confirm this finding in a larger group of subjects. Sixty volunteers with high cholesterol, stratified according to gender and hydroxymethylglutaryl-coenzyme A reductase inhibitor (statin) treatment (yes/no), were randomized to receive 75 g of honey solution or a honey-comparable sugar solution once daily over a period of 14 days. Baseline measurements, including body mass index (BMI) and lipid profile, were obtained, and subjects also completed dietary questionnaires and the Inventory for the Assessment of Negative Bodily Affect-Trait form (INKA-h) questionnaire. Measurements were repeated 2 weeks later. BMI and high-density lipoprotein (HDL) cholesterol values were significantly correlated (r = -0.487; P INKA-h scores and LDL cholesterol values were also significantly correlated (r = 0.273, P = .042). Neither solution influenced significantly cholesterol or triglyceride values in the total group; in women, however, the LDL cholesterol value increased in the sugar solution subgroup but not in the women taking honey. Although ingesting honey did not reduce LDL cholesterol values in general, women may benefit from substituting honey for sugar in their diet. Reducing the BMI lowers the LDL cholesterol value, and psychological interventions also seem important and merit further investigation. PMID:19627212

  9. Lipid Profile and Serum Paraoxonase1 Activity in CRF Patients Pre and Posthemodialysis

    OpenAIRE

    Nitin S. Nagane1* and Jayshree V. Ganu2

    2011-01-01

    Chronic renal failure (CRF) is associated with an increased risk of cardiovascular disease (CVD). In the present study, 30 CRF patients undergoing hemodialysis and 50 healthy controls matching in age and sex were included. We have estimated blood urea, serum creatinine, lipid profile and serum Paraoxonase1 (PON1) activity in pre and post hemodialytic samples. HDL-Cholesterol (HDLC) and serum Paraoxonase1(PON1) activity was found to be significantly reduced (P

  10. Serum total sialic acid, lipid peroxidation, and glutathione reductase levels in patients with rheumatoid arthritis

    OpenAIRE

    Mohan, Surapaneni Krishna; PRIYAV, Vishnu

    2010-01-01

    The changes in the serum sialic acid levels, total lipid peroxidation products (MDA), and glutathione reductase activity were estimated in patients with rheumatoid arthritis. Serum Sialic acid is known as a parameter of inflammation. This work was undertaken to assess the potential role of sialic acid as well as oxidative stress and antioxidant status in patients with rheumatoid arthritis. Materials and methods: The levels of these parameters in serum were studied in 52 subjects with rheuma...

  11. Effect of chronic exposure to cadmium on serum lipid, lipoprotein and oxidative stress indices in male rats

    Directory of Open Access Journals (Sweden)

    Samarghandian Saeed

    2015-09-01

    Full Text Available Cadmium (Cd is an environmental toxic metal implicated in lipid abnormalities. The present study was designed to elucidate the possible association between chronic exposure to Cd concentration and alterations in plasma lipid, lipoprotein, and oxidative stress indices in rats. Sixteen male rats were assigned to 2 groups of 8 rats each (test and control. The Cd-exposed group obtained drinking water containing cadmium chloride (CdCl2 in the concentration of 2.0 mg Cd/L in drinking water for 3 months. At the end of the experimental period, blood samples were obtained to determine the changes of serum triglycerides (TG, total cholesterol (TC, high-density lipoprotein cholesterol (HDL-C, low-density lipoprotein cholesterol (LDL-C, reduced glutathione (GSH, malondialdehyde (MDA and also serum Cd contents. The results of the present study indicated that Cd administration significantly increased the serum levels of TG, TC, LDL-C, MDA and Cd with reduction in the HDL-C and GSH levels. In conclusion, evidence is presented that chronic exposure to low Cd concentration can adversely affect the lipid and lipoprotein profile via lipid peroxidation.

  12. Quantitative Analysis of Serum Lipid Profile in Gallstone Patients and Controls

    International Nuclear Information System (INIS)

    The present study was undertaken to explore the possible role of serum lipid profile in gallstone formation. For this serum lipid profile such as total, free and bound cholesterol, LDL cholesterol, HDL cholesterol, triacylglycerols and total lipids were determined in 109 gallstone patients and 100 controls (matched for age, sex and with negative personal or family history of gallstones) treated at Liaquat University Hospital, Jamshoro, Pakistan. Comparison for serum lipid profile between different groups of gallstone patients and controls revealed no significant variation except for the triacylglycerols and total lipids, which were differed significantly between females of up to 45 and above 45 years age. Comparison for serum lipid profile between pure cholesterol and mixed composition gallstone formers showed no significant difference (p>0.05) between the two groups. The serum lipid profile significantly varied between gallstone patients and controls except bound cholesterol level. Comparison of total cholesterol, free cholesterol, LDL cholesterol, HDL cholesterol, triacylglycerols and total lipids between gallstone patients and controls revealed that there was a significant difference between gallstone patients and controls for (a) females with or without gallstones, (b) females of up to 45 years age and (c) females having more than 3 children. HDL cholesterol is significantly decreased in all the groups of gallstone patients as compared to controls, whereas, bound cholesterol remained non significant in all the groups of gallstone patients when compared with controls. In conclusion, elevated serum total cholesterol, free cholesterol, LDL cholesterol, triacylglycerols and decreased levels of HDL cholesterol seem to play major contributing role in the pathogenesis of gallstones in females of up to 45 years age with more than three children. (author)

  13. Association of rs2072183 SNP and serum lipid levels in the Mulao and Han populations

    Directory of Open Access Journals (Sweden)

    Miao Lin

    2012-05-01

    Full Text Available Abstract Background Niemann-pick C1-like 1 (NPC1L1 is a key protein for intestinal cholesterol transportation. Common single nucleotide polymorphisms (SNPs in the NPC1L1 gene have been associated with cholesterol absorption and serum lipid levels. The present study was undertaken to explore the possible association of NPC1L1 rs2072183 1735 C > G SNP and several environmental factors with serum lipid levels in the Mulao and Han populations. Methods Genotyping of the rs2072183 SNP was performed in 688 subjects of Mulao and 738 participants of Han Chinese. The interactions between NPC1L1 1735 C > G polymorphism and several environmental factors on serum lipid phenotypes were tested using the factorial design covariance analysis after controlling for potential confounders. Results The frequency of G allele was lower in Mulao than in Han (29.72% vs. 37.26%, P P P P P P P P P  Conclusions The present study suggests that the rs2072183 SNP in NPC1L1 gene and its association with serum lipid profiles are different between the Mulao and Han populations. The difference in serum lipid profiles between the two ethnic groups might partly result from different rs2072183 SNP or NPC1L1 gene-environmental interactions.

  14. Serum lipid profile and uric acid levels in preeclampsia in University of Benin Teaching Hospital

    Directory of Open Access Journals (Sweden)

    Nosakhare O Enaruna

    2014-01-01

    Full Text Available Background: Preeclampsia is a pregnancy-specific disease associated with significant maternal and perinatal mortality and morbidity. Lipid abnormality and elevated serum uric acid have been reported as early features of the disease. We aimed to detect the level of serum lipid profile and uric acid abnormalities in severe preeclamptics in Benin City and to measure their clinical significance. Materials and Methods: A prospective case-control study was conducted with subjects presenting with severe preeclampsia to the Obstetric Unit of the UBTH, Benin City. Fasting serum lipid profile and uric acid levels of 40 severe preeclamptic subjects and 80 gestation-matched normotensive controls were done at recruitment. The preeclamptic subjects were managed according to our departmental protocol which included stabilisation and delivery. Their sociodemographic and clinical characteristics were used to generate a database for analysis. Results: The mean serum uric acid level was 28% higher in severe preeclamptics than normotensive women (5.96 ± 2.54 mg/dl versus 4.30 ± 0.85; P = 0.005. There were statistically significant differences in levels of triglycerides (TG, low-density lipoprotein (LDL and high-density lipoprotein (HDL between the preeclamptics and their normotensive controls (P = 0.006, P = 0.000, P = 0.000, respectively. Abnormal serum uric acid was associated with advanced maternal age (P = 0.000, early-onset preeclampsia (P = 0.000 and abnormal body mass index (BMI; P = 0.000. Low birth weight was more likely in preeclamptics with elevated serum uric acid levels (P = 0.041. Conclusion: Abnormality of serum uric acid in preeclampsia was significantly associated with increased frequency of complications but lipid profile abnormalities were not shown in the subjects studied. We recommend a larger scale study to determine lipid profile in normal and complicated pregnancies in our environment.

  15. The Valuable Role of Measuring Serum Lipid Profile in Cancer Progression

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    Farahnaz Ghahremanfard

    2015-09-01

    Full Text Available Objective: Serum lipid levels are not only associated with etiology, but also with prognosis in cancer. To investigate this issue further, we aimed to evaluate the serum levels of lipids in association with the most important prognostic indicators in cancer patients at the start of chemotherapy. Methods: In a retrospective cross-sectional study, using existing medical records obtained from 2009–2014, the data of all incident cancer cases in Iranian patients referred to the Semnan oncology clinic for chemotherapy were analyzed. Data on demographics, cancer type, prognostic indicators (e.g. lymph node involvement, metastasis, and stage of disease, as well as the patient’s lipid profile were collected. We used multiple logistic regression models to show the relationship between prognosis indicators and lipid profile adjusting for age, gender, and type of cancer. Results: The data of 205 patients was gathered. We found a significant difference in the lipid profile between different types of cancers (breast, colon, gastric, and ovarian. With the exception of high-density lipoprotein levels in women, which were higher than in men, the means of other lipid profiles were similar between the genders. There was a significant association between higher levels of low-density lipoprotein (LDL >110mg/dL in the serum and metastasis (adjusted odds ratio=2.4, 95% CI 1.2–3.5. No significant association was reported between lipid profile and lymph nodes involvement and stage of the disease. Conclusion: Our study suggested a benefit of measuring serum levels of lipids for predicting cancer progression. Increased LDL levels can be considered a predictive factor for increasing the risk of metastasis.

  16. Effect of amiodarone, an antiarrhythmic drug, on serum and liver lipids and serum marker enzymes in rats.

    Science.gov (United States)

    Padmavathy, B; Devaraj, S N; Devaraj, H

    1992-12-01

    Administration of amiodarone (AD) to rats leads to marked damage to liver, as evidenced by pathological changes and significant increases in activities of serum marker enzymes and levels of lipids like cholesterol and phospholipids with no alteration in the triglyceride levels. The risk factor, that is the total cholesterol/HDL cholesterol ratio, exhibited increase in the experimental animals, indicating that amiodarone treatment may lead to the development of coronary heart disease. PMID:1294474

  17. Effect of Consumption of Tribulus Terrestris on Serum Glucose and Lipid Levels in Diabetic Rats

    OpenAIRE

    M. Roghani; T Baluchnejad Mojarrad; N Andalibi; F Ansari; M Sharayeli

    2010-01-01

    Introduction: The effect of Tribulus terrestris (TT) on serum glucose and lipid levels was investigated in an experimental model of diabetes mellitus in rats. Methods: Female Wistar rats were divided into control, TT-treated control, diabetic, glibenclamide-treated, and TT-treated diabetic groups. For induction of diabetes, streptozotcin (STZ) was administered (60 mg/Kg). Meanwhile, TT-treated groups received TT mixed with standard pelleted food at a weight ratio of 6.25% for 6 weeks. Serum g...

  18. The effects of coffee on serum lipids and blood pressure in a UK population.

    OpenAIRE

    Lancaster, T; Muir, J; Silagy, C

    1994-01-01

    Data on coffee consumption were collected for 1074 adults attending for health checks in the OXCHECK study, to assess associations with serum lipids and blood pressure. Of the sample, 70.5% drank coffee, largely instant. Coffee had no significant effects on total or high-density lipoprotein (HDL) cholesterol or blood pressure, and was negatively correlated with serum triglycerides. The type of coffee drunk in the UK does not adversely effect these cardiovascular risk factors.

  19. Medication compliance and serum lipid changes in the Helsinki Heart Study.

    OpenAIRE

    Mäenpää, H; Heinonen, O. P.; Manninen, V

    1991-01-01

    1. To control the bias caused by poor medication compliance in the Helsinki Heart Study three methods were used to measure medication compliance during the total 5 years follow up time: continuous capsule counting, semi-annual urine gemfibrozil analysis and a new method, the digoxin marker at the end of the third and fifth study years. 2. The serum lipid responses to gemfibrozil treatment varied linearly with the level of medication compliance, e.g. the mean change in serum total cholesterol ...

  20. Relationship of Lipid Profile and Serum Ferritin levels with Acute Myocardial Infarction

    OpenAIRE

    Shipra; Gupta, Bharat Kumar; Solanki, Ranjan; Punia, Himanshu; Agarwal, Vibhuti; Kaur, Jaskiran; Shukla, Ashish

    2014-01-01

    Introduction: National Health and Nutrition Examination Survey (NHANES III), 1988-1994, first time reported a significant, positive association in iron storage and heart disease risk. Thereafter several researchers have found an association between iron overload, serum ferritin (SF) and MI. No such Indian study was available in the literature and so we decided to find out the relation of lipid profile and Serum Ferritin with myocardial infarction (MI).

  1. Evening primrose oil in rheumatoid arthritis: changes in serum lipids and fatty acids.

    OpenAIRE

    Jäntti, J; Nikkari, T.; Solakivi, T; Vapaatalo, H.; Isomäki, H

    1989-01-01

    The serum concentration of lipids and composition of fatty acids after overnight fasting were studied in 18 patients with rheumatoid arthritis treated for 12 weeks with either 20 ml of evening primrose oil containing 9% of gamma-linolenic acid or olive oil. The serum concentrations of oleic acid, eicosapentaenoic acid, and apolipoprotein B decreased and those of linoleic acid, gamma-linolenic acid, dihomo-gamma-linolenic acid, and arachidonic acid increased during treatment with evening primr...

  2. Gene transfection in high serum levels: case studies with new cholesterol based cationic gemini lipids.

    Directory of Open Access Journals (Sweden)

    Santosh K Misra

    Full Text Available BACKGROUND: Six new cationic gemini lipids based on cholesterol possessing different positional combinations of hydroxyethyl (-CH2CH2OH and oligo-oxyethylene -(CH2CH2On- moieties were synthesized. For comparison the corresponding monomeric lipid was also prepared. Each new cationic lipid was found to form stable, clear suspensions in aqueous media. METHODOLOGY/PRINCIPAL FINDINGS: To understand the nature of the individual lipid aggregates, we have studied the aggregation properties using transmission electron microscopy (TEM, dynamic light scattering (DLS, zeta potential measurements and X-ray diffraction (XRD. We studied the lipid/DNA complex (lipoplex formation and the release of the DNA from such lipoplexes using ethidium bromide. These gemini lipids in presence of a helper lipid, 1, 2-dioleoyl phophatidyl ethanol amine (DOPE showed significant enhancements in the gene transfection compared to several commercially available transfection agents. Cholesterol based gemini having -CH2-CH2-OH groups at the head and one oxyethylene spacer was found to be the most effective lipid, which showed transfection activity even in presence of high serum levels (50% greater than Effectene, one of the potent commercially available transfecting agents. Most of these geminis protected plasmid DNA remarkably against DNase I in serum, although the degree of stability was found to vary with their structural features. CONCLUSIONS/SIGNIFICANCE: -OH groups present on the cationic headgroups in combination with oxyethylene linkers on cholesterol based geminis, gave an optimized combination of new genera of gemini lipids possessing high transfection efficiency even in presence of very high percentage of serum. This property makes them preferential transfection reagents for possible in vivo studies.

  3. The effect of oxidized lipids in the diet on serum lipoprotein peroxides in control and diabetic rats.

    OpenAIRE

    Staprans, I; Rapp, J H; Pan, X. M.; Feingold, K R

    1993-01-01

    The levels of oxidized serum lipoproteins are increased in humans and animals with diabetes. We have examined the contribution of dietary oxidized lipids on the levels of oxidized lipoproteins. In both control and streptozocin induced diabetic rats, the oxidized lipid content of mesenteric lymph chylomicrons (CM) increased when increasing quantities of oxidized lipids were administered intragastrically. However, at all levels of administered oxidized lipids, the quantity of oxidized lipids in...

  4. Trends in serum lipid levels of healthy Chinese in Shanghai from 1973 to 1999

    Institute of Scientific and Technical Information of China (English)

    Haozhu CHEN; Xuejuan JIN; Jun ZHOU; Bin CHEN; Hanzhong ZHUANG; Qingqing HAN

    2008-01-01

    We assessed the secular trends in the serum lipid levels in Shanghai residents from 1973 to 1999. Data from four independent, cross-sectional, population-based surveys were used to analyze the trends in serum lipid levels from 1973 to 1999. A standard protocol was applied across each survey in at least three independent surveys which were separated a 10-year study period. A total of 10733 (1385, 3302, 2399, and 3647 subjects who had complete data on serum lipids in 1973-1974, 1982, 1983 and 1997-1999, respectively) Shanghai residents aged from newborn to 80 years old were enlisted at random. Serum lipid levels were measured in a single laboratory throughout the entire study period. A non-significant increase was noted from 1973-1974 to 1982-1983 in the serum lipid profiles; however, a significant increase was observed from 1982-1983 to 1997-1999 in most of the age groups of both sexes. Mean serum total cholesterol (TC) levels increased significantly (P 40 it was from 5.5% to 11.57%. In females, it was 0 to 4.68% and 6.5% to 16.2%, respectively. In general, the trends in the levels of TC, TG and LDL-c were increasing and trends in the levels of HDL-c were decreasing in most age groups, especially, in the younger age group of Shanghai residents from 1980s to 1990s. These worrying findings are significant for its public health implications. Health education and health pro-motion are necessary to prevent the disease prevalence from rising in the Chinese population.

  5. Analysis on Homocysteine's Risk to Atherosclerosis and Its Correlations with Serum Lipids

    Institute of Scientific and Technical Information of China (English)

    李河; 郭兰; 肖敏; 陈铁峰; 吴书林; 余细勇; 石美铃; 董太明; 刘小清; 黄平; 李义和

    2004-01-01

    Objectives To explore the homocysteine's risk to atherosclerosis and its correlations with serum lipids TG,TG and HDL-C. Methods With a cross sectional study, 490 subjects (aged 41-86 yrs, male 420 and female 70) were surveyed in 1999 in Guangdong Province, China. The main research variables were homocysteine (Hcy) and the serum lipids total cholesterol (TC), triglyceride (TG),high-density lipoprotein cholesterol(HDL-C). Results Hcy was a possible risk factor resulting in atherosclerosis (OR=l.15, 0.05 <P <0.10, n=108) with Logistic regression analysis. There is no correlation or much lower degree correlation between Hey and the serum lipids group of TC, TG, HDL-C. The canonical correlation coefficient between V1 and W1 was R1,Can =0.12(0.05<P<0.10, n=490, V1=Hcy, W1= - 0.9446 * TC + 0.1588 * TG + 0.6009 * HDL-C). Conclusions It is possible that Hcy is a risk factor to atherosclerosis and is independent of serum lipids group or has much lower correlation with it. It is necessary to do more research to explore the risk degree of Hcy inducing atherosclerosis and whether are there are bigger correlations or higher independence between Hcy and other risk factors during the progress of atherosclerosis.

  6. Human Ozone (O3) Exposure Alters Serum Profile of Lipid Metabolites

    Science.gov (United States)

    HUMAN OZONE (O3) EXPOSURE ALTERS SERUM PROFILE OF LIPID METABOLITES Miller, D B.1; Kodavanti, U P.2 Karoly, E D.3; Cascio W.E2, Ghio, A J. 21. UNC-Chapel Hill, Chapel Hill, N.C., United States. 2. NHEERL, U.S. EPA, RTP, N.C., United States. 3. METABOLON INC., Durham, N.C., United...

  7. Effects of Indoor Cycling Associated with Diet on Body Composition and Serum Lipids

    Science.gov (United States)

    do Valle, Valeria S.; de Mello, Danielli B.; Fortes, Marcos de Sa R.; Dantas, Estelio H. M.

    2009-01-01

    Study aim: To determine the effects of indoor cycling training combined with restricted diet, lasting 12 weeks, on serum lipid concentrations in obese women. Material and methods: Twenty women aged 23.8 [plus or minus] 3.6 years were randomly assigned into two groups: control (C) and experimental (E), the latter subjected to indoor cycling at…

  8. Lipoprotein lipase expression, serum lipid and tissue lipid deposition in orally-administered glycyrrhizic acid-treated rats

    Directory of Open Access Journals (Sweden)

    Ton So

    2009-07-01

    Full Text Available Abstract Background The metabolic syndrome (MetS is a cluster of metabolic abnormalities comprising visceral obesity, dyslipidaemia and insulin resistance (IR. With the onset of IR, the expression of lipoprotein lipase (LPL, a key regulator of lipoprotein metabolism, is reduced. Increased activation of glucocorticoid receptors results in MetS symptoms and is thus speculated to have a role in the pathophysiology of the MetS. Glycyrrhizic acid (GA, the bioactive constituent of licorice roots (Glycyrrhiza glabra inhibits 11β-hydroxysteroid dehydrogenase type 1 that catalyzes the activation of glucocorticoids. Thus, oral administration of GA is postulated to ameliorate the MetS. Results In this study, daily oral administration of 50 mg/kg of GA for one week led to significant increase in LPL expression in the quadriceps femoris (p p > 0.05 of the GA-treated rats compared to the control. Decrease in adipocyte size (p > 0.05 in both the visceral and subcutaneous adipose tissue depots accompanies such selective induction of LPL expression. Consistent improvement in serum lipid parameters was also observed, with decrease in serum free fatty acid, triacylglycerol, total cholesterol and LDL-cholesterol but elevated HDL-cholesterol (p > 0.05. Histological analysis using tissue lipid staining with Oil Red O showed significant decrease in lipid deposition in the abdominal muscle and quadriceps femoris (p p > 0.05. Conclusion Results from this study may imply that GA could counteract the development of visceral obesity and improve dyslipidaemia via selective induction of tissue LPL expression and a positive shift in serum lipid parameters respectively, and retard the development of IR associated with tissue steatosis.

  9. The choice of homogenisation equipment affects lipid oxidation in emulsions

    DEFF Research Database (Denmark)

    Horn, Anna Frisenfeldt; Nielsen, Nina Skall; Jensen, Louise Helene Søgaard;

    2012-01-01

    Milk proteins are often used by the food industry because of their good emulsifying properties. In addition, they can also provide oxidative stability to foods. However, different milk proteins or protein components have been shown to differ in their antioxidative properties, and their localisation...... in emulsions has been shown to be affected by the emulsification conditions. The objective of this study was to investigate the influence of homogenisation equipment (microfluidizer vs. two-stage valve homogeniser) on lipid oxidation in 10% fish oil-in-water emulsions prepared with two different milk...

  10. EFFECT OF FEEDING CANOLA AND SOYBEAN OILS ON SERUM LIPID PROFILE IN COMMERCIAL LAYERS

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    Shakoor. H. I., M. L. Khan, Z. Nasir, N. Mukhtar and M. S. Rehman

    2002-04-01

    Full Text Available The purpose of this study was to assess the effect of canola oil and soybean oil on production performance and serum lipid profile in layers. In this study 15 experimental units (8 layers per experimental unit were randomly allotted to 5 different dietary treatments viz control (A. containing 2.5 % canola oil (B, 5% canola oil (C, 2.5% soybean oil (D and 5% soybean oil (E for a period of 9 weeks. Effects of five treatments on production parameters including egg production, egg quality, weight gain and serum lipid profile, serum cholesterol, triglycerides, low-density lipoprotein and high-density lipoprotein were monitored. Serum lipid profile was determined 0.31 and 63 days from start of experiment. Significantly (P<0.05 less serum cholesterol was found in treatment C (295.1 mg/dl as compared with treatment A (321 mg/dl. Low density lipoprotein cholesterol (LDL was significantly (P<0.01 , less in treatment C ( 131.7 mg/dl as compared with treatment A. ( 161 mg/dl and high density lipoprotein cholesterol (HDL was significantly (P<0.01 high in treatment C (31.76 mg/dl as compared with treatment A (25.42 mg/dl and triglyceride (TG was found significantly (P<0.01 less in treatment E ( 907.3 mg/dl as compared with treatment A (960 mg/dl. The results suggested that as the percentage of oils increased in the diet, serum lipid profile showed a positive trend.

  11. The effects of prebiotic, probiotic and synbiotic diets containing Bacillus coagulans and inulin on serum lipid profile in the rat

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    Khadijeh Abhari

    2015-07-01

    Full Text Available An in vivo trial was conducted to evaluate the effects of Bacillus coagulans, and inulin, either separately or in combination, on lipid profile using a rat model. Thirty-two male Wistar rats were randomly divided into four groups (n=8 and fed as follows: standard diet (control, standard diet with 5% w/w long chain inulin (prebiotic, standard diet with 109 spores/day spores of B. coagulans by orogastric gavage (probiotic, and standard diet with 5% w/w long chain inulin and 109 spores/day of B. coagulans (synbiotic. Rats were fed for 30 days. Serum samples were collected 10, 20 and 30 days following onset of treatment. Total, HDL and LDL cholesterol and triglycerides concentrations were analyzed. Results of this study showed that inulin potentially affected the lipid profile. An obvious decrease in serum total cholesterol and LDL-cholesterol of rats fed with inulin in symbiotic and prebiotic groups was seen in all sampling days. Inulin fed rats also demonstrated higher levels of HDL-cholesterol concentration; however this value in probiotic and control fed rats remains without significant change. According to the results of this study, B. coagulans did not contribute to any lipid profile changes after 30 days. Thus, further in vitro investigations on the characteristic of these bacteria could be useful to gain insights into understanding the treatment of probiotics in order to achieve the maximum beneficial effect.

  12. Human serum activates CIDEB-mediated lipid droplet enlargement in hepatoma cells

    International Nuclear Information System (INIS)

    Highlights: •Human serum induced differentiation of hepatoma cells increases cellular lipid droplet (LD) size. •The observed increase in LD size correlates with increased PGC-1α and CIDEB expression. •Induction of CIDEB expression correlates with rescue of VLDL secretion and loss of ADRP. •siRNA knockdown of CIDEB impairs the human serum mediated increase in LD size. •This system represents a cost-efficient model to study CIDEB’s role in lipid biology. -- Abstract: Human hepatocytes constitutively express the lipid droplet (LD) associated protein cell death-inducing DFFA-like effector B (CIDEB). CIDEB mediates LD fusion, as well as very-low-density lipoprotein (VLDL) maturation. However, there are limited cell culture models readily available to study CIDEB’s role in these biological processes, as hepatoma cell lines express negligible levels of CIDEB. Recent work has highlighted the ability of human serum to differentiate hepatoma cells. Herein, we demonstrate that culturing Huh7.5 cells in media supplemented with human serum activates CIDEB expression. This activation occurs through the induced expression of PGC-1α, a positive transcriptional regulator of CIDEB. Coherent anti-Stokes Raman scattering (CARS) microscopy revealed a correlation between CIDEB levels and LD size in human serum treated Huh7.5 cells. Human serum treatment also resulted in a rapid decrease in the levels of adipose differentiation-related protein (ADRP). Furthermore, individual overexpression of CIDEB was sufficient to down-regulate ADRP protein levels. siRNA knockdown of CIDEB revealed that the human serum mediated increase in LD size was CIDEB-dependent. Overall, our work highlights CIDEB’s role in LD fusion, and presents a new model system to study the PGC-1α/CIDEB pathway’s role in LD dynamics and the VLDL pathway

  13. Effects of electromagnetic fields on serum lipids in workers of a power plant.

    Science.gov (United States)

    Wang, Zhaopin; Wang, Lijuan; Zheng, Shuangshuang; Ding, Zheyuan; Liu, Hui; Jin, Wen; Pan, Yifeng; Chen, Zexin; Fei, Ying; Chen, Guangdi; Xu, Zhengping; Yu, Yunxian

    2016-02-01

    This study aimed to evaluate the effects of electromagnetic fields (EMF) exposure on levels of serum lipids in workers of an electric power plant. A cross-sectional study was carried out in an electric power plant in Zhejiang province, China, from August to September 2011. All participants were divided into two groups with high occupational EMF exposure and low occupational EMF exposure. The occupational EMF exposure included radiofrequency EMF and extremely low-frequency EMF. Occupational EMF exposure was associated with an increased level of low-density lipoprotein cholesterol (LDL-c; β = 0.17 mmol/L, P = 0.022). High EMF exposure group with longer employment duration, longer daily EMF exposure duration, and more mobile phone or electric fee per month had significantly higher levels of total cholesterol, LDL-c, or triglyceride than the corresponding reference group. However, significantly decreased level of high-density lipoprotein cholesterol was only observed in high EMF exposure group with more mobile phone fee per month. Similar results were also found in 544 participants with available data of serum lipids in 2010. The findings showed that chronic EMF exposure was associated with the change of serum lipid levels. EMF exposure might modulate the process of lipid metabolism. PMID:26423285

  14. AN INTRA - ABDOMINAL FOREI GN BOCORRELATION OF SERUM LIPID PROFILE, SERUM CALCIUM, ALKALINE PHOSPHATASE AND SERUM PROTEIN WITH HISTOPATHOLOGICAL GRADING AND STAGING IN HEAD AND NECK CANCER

    Directory of Open Access Journals (Sweden)

    Neha

    2014-02-01

    Full Text Available Lipids have a key role in the maintenance of cell integrity. Hypolipidemia is related with increased risk of cancer and mortality. Elevated calcium and alkaline phosphatase has also been seen in cancer patients having m etastasis. Alteration in serum prote ins also has been reported. Hence present study was undertaken in 100 histopathologically confirmed head and neck cancer patients, in Department of Pathology Gandhi Medical College Bhopal, after taking informed written c onsent and ethical clearance. Lipid Profile, Serum Calcium, Alkaline Phosphatase, Serum Protein with Albumin were estimated and compared with age and sex matched healthy controls. Statistical analysis was done using chi square test. Total cholesterol (TC, high density lipoprotein - cholesterol (HDLC, low density lipoprotein - cholesterol (LDLC, Very low density lipoprotein - cholesterol (VLDLC all decreased in head & neck cancer patients. Hypercalcemia was seen in 35% of patients. Raised Alkaline phosphatase (ALP was seen in 8 % cancer patients. Serum calcium was found to be increasing with advancing stage. Further studies should be carried out in large number of patients to confirm the role of these parameters with special attention to modifiable ones and their relation with staging and grading of cancer, which can be used as prognostic markers.

  15. Relationship between abdominal fat distribution assessed by computed tomography and serum lipids in the elderly

    Energy Technology Data Exchange (ETDEWEB)

    Imamura, Toshiharu; Kano, Hiroko; Shin, Kouichi; Konjiki, Ou; Ohsawa, Yoko; Takasaki, Masaru (Tokyo Medical Coll. (Japan))

    1993-02-01

    A nutritional assessment is necessary to evaluate the pathophysiological state of patients, and serum lipids are one of the factors which must be evaluated. Body fat distribution is considered to be associated with cardiac diseases, metabolic diseases and hypertension. In this study, we performed quantitative measurements of fat distribution by X-ray computed tomography (CT) in 31 elderly outpatients. Thirteen men (mean age 74.8 years) and 18 women (mean age 75.4 years) were examined by abdominal CT. All subjects had body mass indices within the normal range and did not have malignant disease, hyperlipidemia, liver dysfunction or diabetes mellitus. CT scans were carried out at the level of the middle abdomen, these scan slices included intraabdominal fat which consisted of omental retroperitoneal and perirenal adipose tissues. Subcutaneous fat areas and intraabdoinal fat areas were measured from six 10-mm-thick slice films using a 2-dimensional computerized calculator. The relationship between serum lipids and fat distribution was also examined. The ratio of intraabdominal adipose tissue area to subcutaneous adipose tissue area (V/S) was higher in men than women. V/S correlated positively with serum triacylgycerol and correlated negatively with serum HDL-Ch. These results suggest that the measurement of body fat distribution is important to evaluate lipid metabolism and nutritional states in the elderly. (author).

  16. Serum lipids and proteins during treatment with a new oral contraceptive combination containing desogestrel.

    Science.gov (United States)

    Penttilä, I M; Bergink, E W; Holma, P; Hulkko, S; Makkonen, M; Pyörälä, T; Castrén, O

    1983-12-01

    The present study was carried out to measure lipid and protein levels in serum of healthy women during treatment with a new oral contraceptive combination containing 0.075 mg desogestrel (Org 2969, 17 alpha-ethinyl-18-methyl-11-methylene-4-estren-17-ol) plus 0.050 mg ethinyloestradiol per tablet. All 30 volunteers took 1 tablet daily for 21 consecutive days, followed by a tablet-free period of 7 days. Treatment lasted 3 months. At the end of treatment serum total cholesterol had increased by 0.26 mmol/l (5.0%), high-density lipoprotein-cholesterol by 0.22 mmol/l (15.2%) and triglycerides by 0.43 mmol/l (50%); the calculated low-density lipoprotein cholesterol had decreased by 0.16 mmol/l (4.9%). All lipid concentrations had returned to initial levels 2 months after treatment stopped. After 3 months treatment serum ceruloplasmin, cortisol-binding globulin capacity, sex-hormone-binding globulin capacity and thyroxine-binding globulin had significantly increased by 85.2, 133, 206 and 101%, respectively. All protein levels returned to normal 2 months after treatment stopped. The relationship between serum lipids and hormone-binding proteins has been discussed, as well as the significance of the high-density lipoprotein level with regard to contraceptive treatment. PMID:6232161

  17. Relationship between abdominal fat distribution assessed by computed tomography and serum lipids in the elderly

    International Nuclear Information System (INIS)

    A nutritional assessment is necessary to evaluate the pathophysiological state of patients, and serum lipids are one of the factors which must be evaluated. Body fat distribution is considered to be associated with cardiac diseases, metabolic diseases and hypertension. In this study, we performed quantitative measurements of fat distribution by X-ray computed tomography (CT) in 31 elderly outpatients. Thirteen men (mean age 74.8 years) and 18 women (mean age 75.4 years) were examined by abdominal CT. All subjects had body mass indices within the normal range and did not have malignant disease, hyperlipidemia, liver dysfunction or diabetes mellitus. CT scans were carried out at the level of the middle abdomen, these scan slices included intraabdominal fat which consisted of omental retroperitoneal and perirenal adipose tissues. Subcutaneous fat areas and intraabdoinal fat areas were measured from six 10-mm-thick slice films using a 2-dimensional computerized calculator. The relationship between serum lipids and fat distribution was also examined. The ratio of intraabdominal adipose tissue area to subcutaneous adipose tissue area (V/S) was higher in men than women. V/S correlated positively with serum triacylgycerol and correlated negatively with serum HDL-Ch. These results suggest that the measurement of body fat distribution is important to evaluate lipid metabolism and nutritional states in the elderly. (author)

  18. Serum metabolites of proanthocyanidin-administered rats decrease lipid synthesis in HepG2 cells.

    Science.gov (United States)

    Guerrero, Ligia; Margalef, Maria; Pons, Zara; Quiñones, Mar; Arola, Lluis; Arola-Arnal, Anna; Muguerza, Begoña

    2013-12-01

    The regular consumption of flavonoids has been associated with reduced mortality and a decreased risk of cardiovascular diseases. The proanthocyanidins found in plasma are very different from the original flavonoids in food sources. The use of physiologically appropriate conjugates of proanthocyanidins is essential for the in vitro analysis of flavonoid bioactivity. In this study, the effect of different proanthocyanidin-rich extracts, which were obtained from cocoa (CCX), French maritime pine bark (Pycnogenol extract, PYC) and grape seed (GSPE), on lipid homeostasis was evaluated. Hepatic human cells (HepG2 cells) were treated with 25 mg/L of CCX, PYC or GSPE. We also performed in vitro experiments to assess the effect on lipid synthesis that is induced by the bioactive GSPE proanthocyanidins using the physiological metabolites that are present in the serum of GSPE-administered rats. For this, Wistar rats were administered 1 g/kg of GSPE, and serum was collected after 2 h. The semipurified serum of GSPE-administered rats was fully characterized by liquid chromatography tandem triple quadrupole mass spectrometry (LC-QqQ/MS(2)). The lipids studied in the analyses were free cholesterol (FC), cholesterol ester (CE) and triglycerides (TG). All three proanthocyanidin-rich extracts induced a remarkable decrease in the de novo lipid synthesis in HepG2 cells. Moreover, GSPE rat serum metabolites reduced the total percentage of CE, FC and particularly TG; this reduction was significantly higher than that observed in the cells directly treated with GSPE. In conclusion, the bioactivity of the physiological metabolites that are present in the serum of rats after their ingestion of a proanthocyanidin-rich extract was demonstrated in Hep G2 cells. PMID:24231101

  19. Co-treatment of chlorpyrifos and lead induce serum lipid disorders in rats: Alleviation by taurine.

    Science.gov (United States)

    Akande, Motunrayo G; Aliu, Yusuf O; Ambali, Suleiman F; Ayo, Joseph O

    2016-07-01

    The aim of this study was to investigate the effects of taurine (TA) on serum lipid profiles following chronic coadministration of chlorpyrifos (CP) and lead acetate (Pb) in male Wistar rats. Fifty rats randomly distributed into five groups served as subjects. Distilled water (DW) was given to DW group, while soya oil (SO; 1 mL kg(-1)) was given to SO group. The TA group was treated with TA (50 mg kg(-1)). The CP + Pb group was administered sequentially with CP (4.25 mg kg(-1); 1/20th median lethal dose (LD50)) and Pb at 233.25 mg kg(-1) (1/20th LD50), while the TA + CP + Pb group received TA (50 mg kg(-1)), CP (4.25 mg kg(-1)), and Pb (233.25 mg kg(-1)) sequentially. The treatments were administered once daily by oral gavage for 16 weeks. The rats were euthanised, and the blood samples were collected at the termination of the study. Sera obtained from the blood samples were analyzed for total cholesterol, high-density lipoprotein cholesterol, triglycerides, and malondialdehyde, and also the activities of serum antioxidant enzymes including superoxide dismutase, catalase and glutathione peroxidase were analyzed. The low-density lipoprotein cholesterol, very-low-density lipoprotein cholesterol, and atherogenic index were calculated. The results showed that CP and Pb induced alterations in the serum lipid profiles and evoked oxidative stress. TA alleviated the disruptions in the serum lipid profiles of the rats partially by mitigating oxidative stress. It was concluded that TA may be used for prophylaxis against serum lipid disorders in animals that were constantly co-exposed to CP and Pb in the environment. PMID:25537622

  20. [Gene polymorphism at apoB locus and the serum lipids profile in children].

    Science.gov (United States)

    Zhu, W; Feng, N; Wang, Y; Ye, G

    2001-09-01

    The relations of gene polymorphism at the apolipoprotein B locus and serum lipid profile in children was studied in 308 normal 7-11 year-old children, including 151 boys and 157 girls. Blood samples were collected for all subjects, and then the serum and blood clot were separated. Serum lipids, including TC, TG, LDL-C, HDL-C, apoB, apoA I and Lp(a) were detected. Genome DNA was extracted from blood clot, then apoB-Xba I gene polymorphism were tested by PCR-RFLP method. The results showed that the distribution of apoB-Xba I genotype in 308 children accorded with Hardy-Weiberg inheritance equilibrium law. The frequency of heterozygote(+/-) was 13.3%, allele(+) was 0.067. The frequency of allele(+) was closed to the internal and Japanese reports (0.033 and 0.04), but much less than the Caucasians (0.50). This showed a ethnic and population difference in the inheritance variation. The average LDL-C levels of the heterozygotes(+/-) were 2.17 mmol/L, no difference compared with homozygotes(-/-) (2.21 mmol/L, P > 0.05). There was also no difference for the genotype distribution between the hyperlipidemia group and control group, which may be the results of no enough sample size and the sample selection, and so on. On the other hand, in normal children, serum lipids controlled by many genes, the effect of a single gene might be small. More studies and analysis on the relationship between serum lipids and multiple genes in multisites should be the next step. PMID:12561593

  1. Clinical significance of serum sex hormones protein and lipid determination in patients with ulcerative colitis

    International Nuclear Information System (INIS)

    Objective: To investigate the relationships between changes of serum sex hormones levels and protein-lipid metabolism in patients with ulcerative colitis. Methods: Serum levels of estradiol (E2) pregnenedione (P), prolactin(PRL), luteinizing hormone (LH), follicle-stimulating hormone (FSH) (with CLIA), sree testos (T, with RIA) and total-protein (TP), albumin (Alb), globulin (G), albumin/globulinratio (A/G) total-cholesterd (TC), high density lipoprotein cholesterols (LDL-C) (with biochemistry were determined in 72 patients) with ulcerative colitis and 72 controls. Results: The serum levels of T, LH, FSH, TP, Alb, A/G, TC, LDL-C in patients with ulcerative colitis were significantly lower than those in controls (P2, PRL in patients with ulcerative colitis were significantly higher than those in controls (P2 were negatively correlated with TP, A/G and TC (P2 levels in the female sex (P>0.05) as well as between LH, FSH and T levels in the male sex (P>0.05). Conclusion: The abnormal serum levels of sex hormone might contribute to the development of hypoproteinaemia and lowered lipid levels in patients with ulcerative colitis. Treatment with correction of serum sex hormones levels might be beneficial to the patients. (authors)

  2. A simple and sensitive method for lipoprotein and lipids profiles analysis of individual micro-liter scale serum samples.

    Science.gov (United States)

    Yang, Liu; Fan, Baoyan; Yang, Kangmin; Zhu, Haibo

    2012-02-01

    A simple and sensitive method to determine lipoprotein and lipids profiles in micro-liter scale individual serum sample is not presently available. Traditional lipoprotein separation techniques either by ultra-centrifugation or by liquid chromatography methods have their disadvantages in both lipoprotein separation and lipids component quantification. In this study we used small volume needing size-exclusion fast protein liquid chromatography to separate different lipoprotein subclasses in 50μL serum. And lipids contents, such as cholesterol, cholesterol ester and triacylglycerol, were measured by using two different fluorescence-based lipid detection methods. With this method, very low density lipoprotein, low density lipoprotein and high density lipoprotein could be easily separated, and follow-up lipid detection was completed by simple kinds of reactions. Serum lipoprotein and lipids profiling from C57BL/6 mice (n=5) and human (n=5) were analyzed. The elution profiles of five individuals were highly reproducible, and there were lipoprotein and lipids distribution variations between C57BL/6 mice and human beings. In conclusion, this method which combined small volume needing size-exclusion fast protein liquid chromatography and fluorescence-based lipids measurement, provided a simple, efficient, integrity and reproducible procedure for determining serum lipoprotein and lipids profiles in micro-liter scale levels. It becomes possible that determination of lipoprotein profiles and gaining information of lipids in different lipoproteins can be accomplished simultaneously. PMID:22155352

  3. Lipidome analysis reveals antifungal polyphenol curcumin affects membrane lipid homeostasis.

    Science.gov (United States)

    Sharma, Monika; Dhamgaye, Sanjiveeni; Singh, Ashutosh; Prasad, Rajendra

    2012-01-01

    This study shows that antifungal curcumin (CUR), significantly depletes ergosterol levels in Candida albicans. CUR while displaying synergy with fluconazole (FLC) lowers ergosterol. However, CUR alone at its synergistic concentration (lower than MIC50), could not affect ergosterol contents. For deeper insight of CUR effects on lipids, we performed high throughput mass spectroscopy (MS) based lipid profiling of C. albicans cells. The lipidome analysis revealed that there were no major changes in phosphoglycerides (PGLs) composition following CUR treatment of Candida, however, significant differences in molecular species of PGLs were detected. Among major SPLs, CUR treatment resulted in the reduction of ceramide and accumulation of IPCs levels. The lipidome of CUR treated cells confirmed a dramatic drop in the ergosterol levels with a simultaneous accumulation of its biosynthetic precursors. This was further supported by the fact that the mutants defective in ergosterol biosynthesis (ERG2 and ERG11) and those lacking the transcription factor regulating ergosterol biosynthesis, UPC2, were highly susceptible to CUR. Our study first time shows that CUR, for its antifungal activity, targets and down regulates delta 5, 6 desaturase (ERG3) resulting in depletion of ergosterol. This results in parallel accumulation of ergosterol biosynthetic precursors, generation of reactive oxygen species (ROS) and cell death. PMID:22201946

  4. Evaluation of Serum, Urine, and Hair Chromium Levels as Indices of Chromium Exposure and the Relationship of these Indices to Serum Lipid and Insulin Levels.

    Science.gov (United States)

    Randall, Janis Avril

    Concentrations of chromium (Cr) in hair, serum, and urine, and serum concentrations of insulin and lipids of a selected group of men exposed to trivalent Cr (Cr III) were compared with those of men not exposed to Cr. Seventy -three tannery workers (TW) (mean age 37 +/- 12 years) from four Southern Ontario tanneries and fifty-two control subjects (CS) (mean age 41 +/- 13 years), matched for age, race, and socioeconomic status, from the Guelph and Toronto areas participated. The median hair and serum Cr concentrations for the TW were significantly higher (p leather tanning industry, is absorbed and retained. Absorption of Cr III had no significant effect on serum insulin concentrations or serum lipid profiles. These results also suggest that concentrations of Cr in hair, serum, and urine are valid biological indices of industrial exposure to Cr III.

  5. Alexithymia, suicide risk and serum lipid levels among adult outpatients with panic disorder.

    Science.gov (United States)

    De Berardis, Domenico; Campanella, Daniela; Serroni, Nicola; Moschetta, Francesco Saverio; Di Emidio, Fabiola; Conti, Chiara; Carano, Alessandro; Acciavatti, Tiziano; Di Iorio, Giuseppe; Martinotti, Giovanni; Siracusano, Alberto; Di Giannantonio, Massimo

    2013-07-01

    To elucidate the relationships between alexithymia, suicide ideation and serum lipid levels in drug-naïve adult outpatients with a DSM-IV diagnosis of Panic Disorder (PD), 72 patients were evaluated. Measures were the Panic Attack and Anticipatory Anxiety Scale, the Toronto Alexithymia Scale (TAS-20), the Scale of Suicide Ideation (SSI) and the Montgomery Åsberg Depression Rating Scale (MADRS). Alexithymic patients showed higher scores on all rating scales and altered serum lipid levels than non-alexithymics. In the hierarchical regression model, the presence of lower HDL-C and higher VLDL-C levels and Difficulty in Identifying Feelings dimension of TAS-20 were associated with higher suicide ideation. In conclusion, alexithymic individuals with PD may show a cholesterol dysregulation that may be linked to suicide ideation. The authors discuss study limitations and future research needs. PMID:23332553

  6. Coronary fat content evaluated by morphometry in patients with severe atherosclerosis has no relation with serum lipid levels

    OpenAIRE

    D.J. Braz Jr.; P.S. Gutierrez; P.L. da Luz

    2007-01-01

    The relationship between lipid serum levels and coronary atherosclerotic plaque fat content was studied in 51 necropsy patients. Serum lipids were measured by standard techniques, during life, in the absence of lipid-lowering drugs. Intima, intimal fat and media areas were measured using a computerized system in cryosections of the odd segments of the right, anterior descending and circumflex coronary arteries stained with Sudan-IV. Mean intimal and lipid areas were 5.74 ± 1.98 and 1.22 ± 0.5...

  7. An Onion Byproduct Affects Plasma Lipids in Healthy Rats

    DEFF Research Database (Denmark)

    Roldan-Marin, E.; Jensen, R. I.; Krath, Britta;

    2010-01-01

    that the controls could be completely separated from OBP, OE, and OR groups in the scores plot and also that OE and OR groups were separated. Plasma lipids and bile acid excretion were the discriminating loading factors for separating OE and OR but also contributed to the separation of onion......Onion may contribute to the health effects associated with high fruit and vegetable consumption. A considerable amount of onion production ends up as waste that might find use in foods. Onion byproduct has not yet been explored for potential health benefits. The aim of this study is to elucidate...... the safety and potential role of onion byproducts in affecting risk markers of cardiovascular disease (CVD). For that purpose, the effects of an onion byproduct, Allium cepa L. cepa 'Recas' (OBP), and its two derived fractions, an ethanolic extract (OE) and a residue (OR), on the distribution of...

  8. Serum and tissue lipids in rats exposed to chronic irradiation with uneven daily doses. Part 2

    International Nuclear Information System (INIS)

    The animals were irradiated continuously for a period of 10 days with a total dose of 9 Gy. Combined hyperlipemia, the accumulation of triacylglycerols in the bone tissue, and a decreased content of triacylglycerols and phospholipids in the thymus were observed. Serum and tissue lipids returned to normal slowly, the accumulation of triacylglycerols in the bone tissue went back to normal only on day 240 after irradiation. (M.D.)

  9. The association of serum lipids with the histological pattern of rectosigmoid adenoma in Taiwanese adults

    OpenAIRE

    Lu Feng-Hwa; Chang Ying-Fang; Yang Yi-Ching; Wu Jin-Shang; Huang Ying-Hsiang; Sun Zih-Jie; Chang Chih-Jen

    2011-01-01

    Abstract Background The mortality rate of colorectal cancer ranks third behind lung and hepatic cancer in Taiwan. Colorectal cancer mostly arises from adenomatous polyps of left colon. The aim of our study was to examine the association of serum lipids with the histological pattern of rectosigmoid adenoma. Methods There were 2,506 eligible examinees aged 20 and above who underwent sigmoidoscopy as a screening examination in National Cheng Kung University Hospital between January 2003 and Octo...

  10. Interaction of lipid vesicle with silver nanoparticle-serum albumin protein corona

    OpenAIRE

    Chen, Ran; Choudhary, Poonam; Schurr, Ryan N.; Bhattacharya, Priyanka; Brown, Jared M.; Chun Ke, Pu

    2012-01-01

    The physical interaction between a lipid vesicle and a silver nanoparticle (AgNP)-human serum albumin (HSA) protein “corona” has been examined. Specifically, the binding of AgNPs and HSA was analyzed by spectrophotometry, and the induced conformational changes of the HSA were inferred from circular dichroism spectroscopy. The fluidity of the vesicle, a model system for mimicking cell membrane, was found to increase with the increased exposure to AgNP-HSA corona, though less pronounced compare...

  11. Effects of Krill Oil on serum lipids of hyperlipidemic rats and human SW480 cells

    Directory of Open Access Journals (Sweden)

    Qian Wen-Bin

    2008-08-01

    Full Text Available Abstract Background Cardiovascular disease (CVD and colon cancer incidence are known to be closely related to dietary factors. This article evaluated effects of krill oil (KO on serum lipids of hyperlipidemia rats and human colon cancer cells (SW480. Serum lipids of rats fed with high fat diet (HFD and different doses of KO were measured by automatic analyzer. Effect of KO on viability of cells was determined by methyl thiazolyl tetrazolium (MTT assay. Results Except for higher dose group, body weights decreased significantly. Total cholesterol (TC, LDL-cholesterol (LDL-C of all dose groups, Triglycerides (TG of low and mid dose groups descended significantly, while there were no significant differences of HDL-cholesterol (HDL-C, compared with control group. Treatment of colon cancer cells with KO also resulted in time-dependent inhibition of cell growth. Conclusion Our findings indicated that the consumption of KO may provide benefits to control serum lipid levels in certain diseases and inhibit growth of colon cancer cells. Therefore, KO may be a good candidate for development as a functional food and nutraceutical.

  12. Serum levels of lipids, calcium and magnesium in women with hypothyroidism and cardiovascular diseases

    Directory of Open Access Journals (Sweden)

    Hussein Kadhem Al-Hakeim

    2009-01-01

    Full Text Available Lipid abnormalities in hypothyroidism contribute to the disproportionate increase in cardiovascular risk. A possible relationship between serum level of magnesium (Mg and calcium (Ca and cardiovascular disease was recorded. In this work, the possible correlation between lipid profile components and serum cations Ca and Mg was investigated. Matched healthy women were evaluated in a cross-sectional study. All parameters were measured spectrophotometrically. The results showed a significant decrease (P < 0.05 in high-density lipoprotein-cholesterol (HDL-C, total and ionized Mg in hypothyroid patients in comparing with control group. There was a significant increase (P < 0.05 in serum total cholesterol (TC, low-density lipoprotein-cholesterol (LDL-C and (LDL-C/(HDL-C ratio in hypothyroid patients as compared with control group. However, no correlation was found between the cation levels and lipid profile of the studied groups. It can be concluded that patients with hypothyroidism exhibited elevated atherogenic parameters (TC and LDL-C and high risk of cardiovascular diseases.

  13. Determination of serum aluminum, platelet aggregation and lipid peroxidation in hemodialyzed patients

    Directory of Open Access Journals (Sweden)

    T.J.C. Neiva

    2002-03-01

    Full Text Available Aluminum (Al3+ overload is frequently associated with lipid peroxidation and neurological disorders. Aluminum accumulation is also reported to be related to renal impairment, anemia and other clinical complications in hemodialysis patients. The aim of the present study was to determine the degree of lipid peroxidation, platelet aggregation and serum aluminum in patients receiving regular hemodialytic treatment. The level of plasma lipid peroxidation was evaluated on the basis of thiobarbituric acid reactive substances (TBARS. Mean platelet peroxidation in patients undergoing hemodialysis was significantly higher than in normal controls (2.7 ± 0.03 vs 1.8 ± 0.06 nmol/l, P<0.05. Platelet aggregation and serum aluminum levels were determined by a turbidimetric method and atomic absorption spectrophotometry, respectively. Serum aluminum was significantly higher in patients than in normal controls (44.5 ± 29 vs 10.8 ± 2.5 µg/l, P<0.05. Human blood platelets were stimulated with collagen (2.2 µg/ml, adenosine diphosphate (6 µM and epinephrine (6 µM and showed reduced function with the three agonists utilized. No correlation between aluminum levels and platelet aggregation or between aluminum and peroxidation was observed in hemodialyzed patients.

  14. A STUDY ON ALTER ATION OF SERUM LIPID PROFILE IN GESTATIONAL PROTEINURIC HYPERTENSION

    Directory of Open Access Journals (Sweden)

    Evelyn

    2015-08-01

    Full Text Available BACKGROUND: Changes in lipid metabolism occurs in normal pregnancy during third trimester. This dyslipidemia indicative of insulin resistance is accentuated with Pre - eclampsia and Eclampsia. It contributes to both the pathogenesis of pre - eclampsia and to the risk of later - life cardiovascular disease in these patients. I have attempted to study the alterations in lipid profile in normal pregnancy, pre - eclampsia and eclampsia. MATERIALS AND METHODS: This study was conducted in Govt . Medical College for a period of 6 months. 25 normotensive non - pregnant women, 25 normal pregnant women, 25 pre eclampsia patients and 25 eclampsia patients were selected All the antenatal were in third trimester. Serum lipid profile was determined. Data analysis was done by excel sheet and SPSS software. RESULTS : Serum Triglycerides, VLDL, Total Cholesterol, LDL Cholesterol and HDL Cholesterol were significantly elevated in last trimester of normal pregnancy when compared to normal non - pregnant women. Also a significant increase in serum triglycerides and VLDL were seen in pre - eclampsia when compared to that of normal pregnancy. Also a significant decrease in HDL Cholesterol levels were seen in eclampsia when compared to normal pregnancy. CONCLUSION : Dyslipidemia suggestive of an exaggerated insulin resistance was found in pre eclampsia and eclampsia. Early interventions like prevention of obesity, exercise and balanced diet can reduce the risk of future cardiovascular morbidity and mortality in these patients.

  15. Acute effects of moderate exercise on serum lipids, lipoproteins and apolipoproteins in sedentary young women.

    Science.gov (United States)

    Imamura, H; Katagiri, S; Uchid, K; Miyamoto, N; Nakano, H; Shirota, T

    2000-12-01

    1. The aim of the present study was to examine the effects of moderate exercise on serum lipids, lipoproteins and apolipoproteins in seven sedentary young women under controlled conditions. 2. The subjects exercised on separate days for 30 or 60 min at an intensity of 60% of maximal oxygen uptake on a cycle ergometer. Total cholesterol, triglycerides, high-density lipoprotein-cholesterol (HDL-C), HDL2-C, HDL3-C, low-density lipoprotein-cholesterol, apolipoproteins A-I, A-II and B were measured in the serum at the end of the 60 min rest period before each exercise, immediately after the performance of each exercise and at 30 min and 1, 2 and 24 h after each exercise. 3. The results showed that there were no significant differences between the pre- and postexercise samples for any of the parameters tested. 4. The results of the present study suggest that a single bout of exercise designed to simulate a typical training workout has no noticeable effect on serum lipids, lipoproteins and apolipoproteins in normal sedentary young women who have normal lipid profiles, are in the follicular phase of their menstrual cycle and who consume a relatively low-fat diet. PMID:11117233

  16. Serum lipid profile in oral squamous cell carcinoma: alterations and association with some clinicopathological parameters and tobacco use.

    Science.gov (United States)

    Acharya, S; Rai, P; Hallikeri, K; Anehosur, V; Kale, J

    2016-06-01

    Hypocholesterolemia has been observed in patients with cancers of various organs; however the potential role of alterations in serum lipid profile in oral cancer remains controversial. Hence, this study aimed to evaluate the serum lipid profile in oral squamous cell carcinoma (OSCC) and its prognostic significance. Ninety untreated OSCC patients, who reported to the craniofacial unit for treatment between 2011 and 2014, were identified to obtain clinicopathological data and preoperative blood investigations including lipid profile. The fasting blood lipid profile, including total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), and low density lipoprotein (LDL), was evaluated using a fully automated biochemistry analyser. Data were analyzed statistically using the Student's t-test, analysis of variance, and post hoc tests. Statistically significant decreases in serum TC, HDL, and LDL levels were observed in OSCC patients as compared to healthy controls (P<0.05). There was no statistically significant difference in mean lipid profile values in terms of stage, grade, or lymph node metastasis. This study identified changes in lipid profiles in OSCC. The results suggest that during the development and progression of OSCC, levels of serum lipids are decreased. A review of the literature confirmed that OSCC patients exhibit aberrant serum lipid patterns. PMID:26899131

  17. The effect of a fish diet on serum lipids in healthy human subjects.

    Science.gov (United States)

    von Lossonczy, T O; Ruiter, A; Bronsgeest-Schoute, H C; van Gent, C M; Hermus, R J

    1978-08-01

    A cross-over study was done with 19 male and 23 female volunteers living in a monastery and a convent, respectively. The effect of a fat gish (mackerel) diet on the blood serum lipid composition was studied. As the normal diet of these volunteers was of the lacto-ovo-vegetarian type, a control diet in which the fish was replaced by full-fat cheese was used. Subjects consuming the fish diet had a daily uptake of polyunsaturated acids of the omega3 family of about 8 g; comparable amounts of linoleic acid were ingested with both diets. Both diets were consumed for a period of 3 weeks. Serum cholesterol was slightly but significantly (7.5%) lower and serum triglycerides considerably lower (35%) on the fish diet, whereas high density lipoprotein cholesterol increased slightly. Lipoprotein analysis showed a strong very low density lipoprotein decrease by the fish diet while, in the men, both low and high density lipoproteins increased. The fatty acid composition of serum lipids showed considerable differences; C20:5omega3 increased in all fractions and C22:6omega3 was found in the triglycerides and the phospholipids, but not in the sterol esters. These increases occurred chiefly at the expense of C18:1omega9 and, in particular, C18:2omega6, which indicates a replacement of omega6 by omega3 acids. Long-chain monoenoic acids which are abundant in the mackerel were not detected in any serum lipid fraction. PMID:567008

  18. Cholesterol derived cationic lipids as potential non-viral gene delivery vectors and their serum compatibility.

    Science.gov (United States)

    Ju, Jia; Huan, Meng-Lei; Wan, Ning; Hou, Yi-Lin; Ma, Xi-Xi; Jia, Yi-Yang; Li, Chen; Zhou, Si-Yuan; Zhang, Bang-Le

    2016-05-15

    Cholesterol derivatives M1-M6 as synthetic cationic lipids were designed and the biological evaluation of the cationic liposomes based on them as non-viral gene delivery vectors were described. Plasmid pEGFP-N1, used as model gene, was transferred into 293T cells by cationic liposomes formed with M1-M6 and transfection efficiency and GFP expression were tested. Cationic liposomes prepared with cationic lipids M1-M6 exhibited good transfection activity, and the transfection activity was parallel (M2 and M4) or superior (M1 and M6) to that of DC-Chol derived from the same backbone. Among them, the transfection efficiency of cationic lipid M6 was parallel to that of the commercially available Lipofectamine2000. The optimal formulation of M1 and M6 were found to be at a mol ratio of 1:0.5 for cationic lipid/DOPE, and at a N/P charge mol ratio of 3:1 for liposome/DNA. Under optimized conditions, the efficiency of M1 and M6 is greater than that of all the tested commercial liposomes DC-Chol and Lipofectamine2000, even in the presence of serum. The results indicated that M1 and M6 exhibited low cytotoxicity, good serum compatibility and efficient transfection performance, having the potential of being excellent non-viral vectors for gene delivery. PMID:27072908

  19. Environmentally Relevant Dose of Bisphenol A Does Not Affect Lipid Metabolism and Has No Synergetic or Antagonistic Effects on Genistein’s Beneficial Roles on Lipid Metabolism

    Science.gov (United States)

    Fan, Ying; Li, Hongyu; Zhao, Nana; Yang, Huiqin; Ye, Xiaolei; He, Dongliang; Yang, Hui; Jin, Xin; Tian, Chong; Ying, Chenjiang

    2016-01-01

    Both bisphenol A (BPA, an endocrine disrupting chemicals) and genistein (a phytoestrogen mainly derived from leguminosae) are able to bind to estrogen receptors, but they are considered to have different effects on metabolic syndrome, surprisingly. We here investigate the effects of an environmentally relevant dose of BPA alone and the combined effects with genistein on lipid metabolism in rats. Eight groups of adult male Wistar rats, fed with either standard chow diet or high-fat diet, were treated with BPA (50μg/kg/day), genistein (10mg/kg/day), and BPA plus genistein for 35 weeks, respectively. Metabolic parameters in serum and liver were determined; the hematoxylin/eosin and oil Red O staining were used to observe liver histologically; gene expressions related to hepatic lipid metabolism were analyzed by Real-time PCR; protein expressions of PPARγ, PPARα and LC3 in liver were analyzed by western blotting. No difference of body weight gain, total energy intake, liver weight/body weight or body fat percentage in both STD- and HFD-fed sub-groups was observed after treatment with BPA, genistein, or BPA plus genistein (P>0.05). Genistein alleviated lipid metabolism disorder and decreased the mRNA and protein expression of PPARγ (P0.05) or combined with genistein. Our findings suggest that long-term environmentally relevant dose of BPA did not affect lipid metabolism, and had no synergetic or antagonistic roles on genistein’s beneficial function on hepatic lipid metabolism. PMID:27171397

  20. EFFECTS OF PSIDIUM GUAJAVA AQUEOUS EXTRACTS ON HAEMATOLOGICAL PROFILE AND SERUM LIPID VARIABLES OF ALBINO RATS

    Directory of Open Access Journals (Sweden)

    M. P. SINHA

    2013-01-01

    Full Text Available The effect of aqueous extract of leaves on some haematological and serum lipid parameters in rats during a sevenday administration of the doses of 250mg/kg and 500 mg/kg body weight orally was investigated. The parametersevaluated include serum lipids, red and white blood cell indices. The results show that the extract administeredsignificantly increased (p<0.05 packed cell volume, haemoglobin concentration, red blood cell, MCH, MCHC,MCV and platelet count t at the dose of 250mg /kg and 500 mg/kg body weight when compared with control.Whereas Psidium guajava the platelet was significantly increased (p<0.05 at 250mg/kg body weight but at500mg/kg body weight the count significanty reduced (p<0.05. Also, the extract significantly increased (p<0.05white blood cell count at all doses administered when compared with control. Moreover, the extract significantlyreduced (p<0.05 total cholesterol concentration, triglycerides and HDL-cholesterol concentration in the serumwhile it had no significant effect on serum LDL-cholesterol concentration at all doses administered when comparedwith controls. The results of this study suggest that the extract may have beneficial effect on serum cholesterolconcentration and triglycerides reduction as well as in anemia and immunity dependent disorders.

  1. Mechanics of lipid bilayer junctions affecting the size of a connecting lipid nanotube

    Science.gov (United States)

    Karlsson, Roger; Kurczy, Michael; Grzhibovskis, Richards; Adams, Kelly L.; Ewing, Andrew G.; Cans, Ann-Sofie; Voinova, Marina V.

    2011-06-01

    In this study we report a physical analysis of the membrane mechanics affecting the size of the highly curved region of a lipid nanotube (LNT) that is either connected between a lipid bilayer vesicle and the tip of a glass microinjection pipette (tube-only) or between a lipid bilayer vesicle and a vesicle that is attached to the tip of a glass microinjection pipette (two-vesicle). For the tube-only configuration (TOC), a micropipette is used to pull a LNT into the interior of a surface-immobilized vesicle, where the length of the tube L is determined by the distance of the micropipette to the vesicle wall. For the two-vesicle configuration (TVC), a small vesicle is inflated at the tip of the micropipette tip and the length of the tube L is in this case determined by the distance between the two interconnected vesicles. An electrochemical method monitoring diffusion of electroactive molecules through the nanotube has been used to determine the radius of the nanotube R as a function of nanotube length L for the two configurations. The data show that the LNT connected in the TVC constricts to a smaller radius in comparison to the tube-only mode and that tube radius shrinks at shorter tube lengths. To explain these electrochemical data, we developed a theoretical model taking into account the free energy of the membrane regions of the vesicles, the LNT and the high curvature junctions. In particular, this model allows us to estimate the surface tension coefficients from R( L) measurements.

  2. Mechanics of lipid bilayer junctions affecting the size of a connecting lipid nanotube

    Directory of Open Access Journals (Sweden)

    Voinova Marina

    2011-01-01

    Full Text Available Abstract In this study we report a physical analysis of the membrane mechanics affecting the size of the highly curved region of a lipid nanotube (LNT that is either connected between a lipid bilayer vesicle and the tip of a glass microinjection pipette (tube-only or between a lipid bilayer vesicle and a vesicle that is attached to the tip of a glass microinjection pipette (two-vesicle. For the tube-only configuration (TOC, a micropipette is used to pull a LNT into the interior of a surface-immobilized vesicle, where the length of the tube L is determined by the distance of the micropipette to the vesicle wall. For the two-vesicle configuration (TVC, a small vesicle is inflated at the tip of the micropipette tip and the length of the tube L is in this case determined by the distance between the two interconnected vesicles. An electrochemical method monitoring diffusion of electroactive molecules through the nanotube has been used to determine the radius of the nanotube R as a function of nanotube length L for the two configurations. The data show that the LNT connected in the TVC constricts to a smaller radius in comparison to the tube-only mode and that tube radius shrinks at shorter tube lengths. To explain these electrochemical data, we developed a theoretical model taking into account the free energy of the membrane regions of the vesicles, the LNT and the high curvature junctions. In particular, this model allows us to estimate the surface tension coefficients from R(L measurements.

  3. Factors affecting Archaeal Lipid Compositions of the Sulfolobus Species

    Science.gov (United States)

    He, L.; Han, J.; Wei, Y.; Lin, L.; Wei, Y.; Zhang, C.

    2010-12-01

    Temperature is the best known variable affecting the distribution of the archaeal glycerol dibiphytanyl glycerol tetraethers (GDGTs) in marine and freshwater systems. Other variables such as pH, ionic strength, or bicarbonate concentration may also affect archaeal GDGTs in terrestrial systems. Studies of pure cultures can help us pinpoint the specific effects these variables may have on archaeal lipid distribution in natural environments. In this study, three Sulfolobus species (HG4, HB5-2, HB9-6) isolated from Tengchong hot springs (pH 2-3, temperature 73-90°C) in China were used to investigate the effects of temperature, pH, substrate, and type of strain on the composition of GDGTs. Results showed that increase in temperature had negative effects on the relative contents of GDGT-0 (no cyclopentyl rings), GDGT-1 (one cyclopentyl ring), GDGT-2 and GDGT-3 but positive effects on GDGT-4, GDGT-4', GDGT-5 and GDGT-5'. Increase in pH, on the other hand, had negative effects on GDGT-0, GDGT-1, GDGT-4', GDGT-5 and GDGT-5', and positive effects on GDGT-3 and GDGT-4. GDGT-2 remained relatively constant with changing pH. When the HG4 was grown on different substrates, GDGT-5 was five time more abundant in sucrose-grown cultures than in yeast extract- or sulfur- grown cultures, suggesting that carbohydrates may stimulate the production of GDGT-5. For all three species, the ring index (average number of rings) of GDGTs correlated positively with incubation temperature. In HG4, ring index was much lower at optimal pH (3.5) than at other pH values. Ring index of HB5-2 or HB9-6 is higher than that of HG4, suggesting that speciation may affect the degree of cyclization of GDGT of the Sulfolobus. These results indicate that individual archaeal lipids respond differently to changes in environmental variables, which may be also species specific.

  4. The effect of modern Balinese Baris dancing exercise (MBBDE) on serum lipid profiles.

    Science.gov (United States)

    Adiputra, N; Djojonegoro, S; Soekarman; Manuaba, A

    1992-12-01

    There is still a lack of information on the effect of regular dancing exercise on lipid profiles. On the other hand, many studies have been carried out on the effect of aerobic exercise on lipid profiles. This study tried to find out the effects of Modern Balinese Baris Dancing Exercise (MBBDE) on serum lipid profiles. Subjects of the study were 30 healthy young male Balinese as an experimental group, and another 30 healthy young Balinese as control group. The MBBDE involved exercise intensity at 70-80% of targeted heart rate, for 50 min period, 3 times per week for 8 weeks. Pre- and post-control group design was applied. Total cholesterol and triglyceride were measured enzymatically. Following MBBDE 3 x 50 min/week for 8 weeks duration, serum level of high density lipoprotein cholesterol (HDL-C) concentration increased significantly from 55.3 +/- 2.32 mg/dl to 63.2 +/- 2.82 mg/dl (p < 0.001). It was also associated with the decrease of total cholesterol concentration from 195.5 +/- 21.10 mg/dl to 161.8 +/- 21.29 mg/dl (p < 0.001); triglyceride concentration from 132.2 +/- 9.65 mg/dl to 110.6 +/- 9.08 mg/dl (p < 0.001); and low density lipoprotein cholesterol (LDL-C) concentration from 113.8 +/- 21.68 mg/dl to 76.9 +/- 20.76 mg/dl (p < 0.001). No significant differences were found in the above parameters in the control group. It is concluded that MBBDE is an aerobic, endurance exercise, and therefore produces beneficial effect on the serum lipid profiles. PMID:1340496

  5. Scoparone affects lipid metabolism in primary hepatocytes using lipidomics.

    Science.gov (United States)

    Zhang, Aihua; Qiu, Shi; Sun, Hui; Zhang, Tianlei; Guan, Yu; Han, Ying; Yan, Guangli; Wang, Xijun

    2016-01-01

    Lipidomics, which focuses on the global study of molecular lipids in biological systems, could provide valuable insights about disease mechanisms. In this study, we present a nontargeted lipidomics strategy to determine cellular lipid alterations after scoparone exposure in primary hepatocytes. Lipid metabolic profiles were analyzed by high-performance liquid chromatography coupled with time-of-flight mass spectrometry, and a novel imaging TransOmics tool has been developed for the analysis of high-resolution MS data, including the data pretreatment, visualization, automated identification, deconvolution and quantification of lipid species. Chemometric and statistical analyses of the obtained lipid fingerprints revealed the global lipidomic alterations and tested the therapeutic effects of scoparone. Identification of ten proposed lipids contributed to the better understanding of the effects of scoparone on lipid metabolism in hepatocytes. The most striking finding was that scoparone caused comprehensive lipid changes, as represented by significant changes of the identificated lipids. The levels of identified PG(19:1(9Z)/14:0), PE(17:1(9Z)/0:0), PE(19:1(9Z)/0:0) were found to be upregulated in ethanol-induced group, whereas the levels in scoparone group were downregulated. Lipid metabolism in primary hepatocytes was changed significantly by scoparone treatment. We believe that this novel approach could substantially broaden the applications of high mass resolution mass spectrometry for cellular lipidomics. PMID:27306123

  6. Investigation the effect of Commiphora mukul on blood glucose and Serum lipid profile in diabetic rats

    Directory of Open Access Journals (Sweden)

    leila Shirazi

    2015-01-01

    Full Text Available Background & aim: The decrease of serum glucose level and lipids in diabetic patients is clinically significant. The purpose of this study was to determine the effect of Commiphora mukul extract on blood sugar and lipid profile in diabetic rats. Methods: In the present experimental study, thirty-two male Wistar rats were randomly divided into four groups: control, control treated with the extract, diabetic and diabetic treated with the extract. Diabetes was induced by intraperitoneal injection of Streptozotocin. In an eight week period, the control group of normal saline and the control group and diabetic recipient extract of CM oleo gum blue resin was given by gavage. Treatment resumed eight weeks with onset of hyperglycemia. The control and diabetic control groups received normal saline orally. Extract treated control and extract treated diabetic groups received extract of Commiphora mukul gum (300 mg/kg P.O. daily by gavage. At the end of this period, blood samples were collected from each rat and biochemical tests for investigation of glucose level and lipid profile was performed. One- way analysis of variance (ANOVA statistical test and Post-hoc test Tukey’s were used for data analysis Results: The study indicated that diabetes increases the serum levels of glucose, cholesterol, triglycerides, LDL and HDL. Administration of Commiphora mukul gum extract in diabetic groups significantly decreased the serum level of glucose, cholesterol(p<0.01 and triglyceride and LDL(p<0.001 and increased HDL(p<0.01. Conclusion: Commiphora mukul gum extract may well improve undesirable effects of diabetes on serum level of glucose, cholesterol, triglyceride, LDL and HDL.

  7. Associations between the human intestinal microbiota, Lactobacillus rhamnosus GG and serum lipids indicated by integrated analysis of high-throughput profiling data

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    Leo Lahti

    2013-02-01

    Full Text Available Accumulating evidence indicates that the intestinal microbiota regulates our physiology and metabolism. Bacteria marketed as probiotics confer health benefits that may arise from their ability to affect the microbiota. Here high-throughput screening of the intestinal microbiota was carried out and integrated with serum lipidomic profiling data to study the impact of probiotic intervention on the intestinal ecosystem, and to explore the associations between the intestinal bacteria and serum lipids. We performed a comprehensive intestinal microbiota analysis using a phylogenetic microarray before and after Lactobacillus rhamnosus GG intervention. While a specific increase in the L. rhamnosus-related bacteria was observed during the intervention, no other changes in the composition or stability of the microbiota were detected. After the intervention, lactobacilli returned to their initial levels. As previously reported, also the serum lipid profiles remained unaltered during the intervention. Based on a high-resolution microbiota analysis, intake of L. rhamnosus GG did not modify the composition of the intestinal ecosystem in healthy adults, indicating that probiotics confer their health effects by other mechanisms. The most prevailing association between the gut microbiota and lipid profiles was a strong positive correlation between uncultured phylotypes of Ruminococcus gnavus-group and polyunsaturated serum triglycerides of dietary origin. Moreover, a positive correlation was detected between serum cholesterol and Collinsella (Coriobacteriaceae. These associations identified with the spectrometric lipidome profiling were corroborated by enzymatically determined cholesterol and triglyceride levels. Actinomycetaceae correlated negatively with triglycerides of highly unsaturated fatty acids while a set of Proteobacteria showed negative correlation with ether phosphatidylcholines. Our results suggest that several members of the Firmicutes

  8. Associations between the human intestinal microbiota, Lactobacillus rhamnosus GG and serum lipids indicated by integrated analysis of high-throughput profiling data.

    Science.gov (United States)

    Lahti, Leo; Salonen, Anne; Kekkonen, Riina A; Salojärvi, Jarkko; Jalanka-Tuovinen, Jonna; Palva, Airi; Orešič, Matej; de Vos, Willem M

    2013-01-01

    Accumulating evidence indicates that the intestinal microbiota regulates our physiology and metabolism. Bacteria marketed as probiotics confer health benefits that may arise from their ability to affect the microbiota. Here high-throughput screening of the intestinal microbiota was carried out and integrated with serum lipidomic profiling data to study the impact of probiotic intervention on the intestinal ecosystem, and to explore the associations between the intestinal bacteria and serum lipids. We performed a comprehensive intestinal microbiota analysis using a phylogenetic microarray before and after Lactobacillus rhamnosus GG intervention. While a specific increase in the L. rhamnosus-related bacteria was observed during the intervention, no other changes in the composition or stability of the microbiota were detected. After the intervention, lactobacilli returned to their initial levels. As previously reported, also the serum lipid profiles remained unaltered during the intervention. Based on a high-resolution microbiota analysis, intake of L. rhamnosus GG did not modify the composition of the intestinal ecosystem in healthy adults, indicating that probiotics confer their health effects by other mechanisms. The most prevailing association between the gut microbiota and lipid profiles was a strong positive correlation between uncultured phylotypes of Ruminococcus gnavus-group and polyunsaturated serum triglycerides of dietary origin. Moreover, a positive correlation was detected between serum cholesterol and Collinsella (Coriobacteriaceae). These associations identified with the spectrometric lipidome profiling were corroborated by enzymatically determined cholesterol and triglyceride levels. Actinomycetaceae correlated negatively with triglycerides of highly unsaturated fatty acids while a set of Proteobacteria showed negative correlation with ether phosphatidylcholines. Our results suggest that several members of the Firmicutes, Actinobacteria and

  9. Lipid Profile and Serum Paraoxonase1 Activity in CRF Patients Pre and Posthemodialysis

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    Nitin S. Nagane1* and Jayshree V. Ganu2

    2011-01-01

    Full Text Available Chronic renal failure (CRF is associated with an increased risk of cardiovascular disease (CVD. In the present study, 30 CRF patients undergoing hemodialysis and 50 healthy controls matching in age and sex were included. We have estimated blood urea, serum creatinine, lipid profile and serum Paraoxonase1 (PON1 activity in pre and post hemodialytic samples. HDL-Cholesterol (HDLC and serum Paraoxonase1(PON1 activity was found to be significantly reduced (P<0.001 in CRF patients as compared to that of controls. However HDL-Cholesterol (HDLC and Paraoxonase1 (PON1 show significant elevation (P<0.001 post hemodialysis than prehemodialytic samples of CRF patients. The levels of blood urea, serum creatinine, total cholesterol, LDL-Cholesterol (LDLC and Triglyceride (TG were significantly elevated (P<0.001 in CRF patients. After hemodialysis these parameters were significantly reduced (P<0.001 as compared to pre hemodialytic samples. The low HDL-Cholesterol (HDLC and serum Paraoxonase1 (PON1 activity may be responsible factor for cardiovascular disease associated with CRF. Therefore, management of CRF patients by Hemodialysis (HD has beneficial effect.

  10. Analysis of polar lipids in the serum from rats fed shiitake by liquid chromatography-mass spectrometry/sass spectrometry

    Science.gov (United States)

    Consumption of a shiitake mushroom diet has been reported to have effects on serum phospholipids. However, much less is known about the effect on serum polar lipids, including lysophospholipids and free fatty acids. In the present study, the effects of a shiitake diet were evaluated on the basis of ...

  11. Beneficial effects of raloxifene and atorvastatin on serum lipids and HDL phospholipids levels of postmenopausal women.

    Science.gov (United States)

    Piperi, Christina; Kalofoutis, C; Skenderi, Katerina; Economidou, Olga; Kalofoutis, A

    2004-06-01

    Selective oestrogen receptor modulators (raloxifene) and statins (atorvastatin) have been shown to reduce the risk of cardiovascular disease associated with the postmenopausal status. Their beneficial effects may be mediated partly by favourable changes in serum lipids and particular on HDL phospholipid composition. In the present study, individual administration of either raloxifene (Group A) or atorvastatin (Group B) or both (Group C) was compared for a period of 3 months and their effects on total lipids and HDL phospholipids were evaluated. The combined treatment of raloxifene and atorvastatin resulted in profound changes in the majority of serum lipids, including a significant reduction in total cholesterol and triglycerides (P<0.001), a rise in total phospholipids (P<0.01) and a reduction in LDL-C and Apo B levels (P<0.001). Furthermore, Apo A-I was elevated (P<0.001) whereas total HDL phospholipids were significantly increased (P<0.05). Specifically, HDL phosphatidylcholine levels were markedly increased (P<0.001) and HDL lysophosphatidylcholine, sphingomyelin and phosphatidylinositol levels were reduced (P<0.05). A further attempt to evaluate each treatment group was performed and the significance of these results is discussed. PMID:15203583

  12. Serum lipid profiles are associated with disability and MRI outcomes in multiple sclerosis

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    Weinstock-Guttman Bianca

    2011-10-01

    Full Text Available Abstract Background The breakdown of the blood-brain-barrier vascular endothelium is critical for entry of immune cells into the MS brain. Vascular co-morbidities are associated with increased risk of progression. Dyslipidemia, elevated LDL and reduced HDL may increase progression by activating inflammatory processes at the vascular endothelium. Objective To assess the associations of serum lipid profile variables (triglycerides, high and low density lipoproteins (HDL, LDL and total cholesterol with disability and MRI measures in multiple sclerosis (MS. Methods This study included 492 MS patients (age: 47.1 ± 10.8 years; disease duration: 12.8 ± 10.1 years with baseline and follow-up Expanded Disability Status Score (EDSS assessments after a mean period of 2.2 ± 1.0 years. The associations of baseline lipid profile variables with disability changes were assessed. Quantitative MRI findings at baseline were available for 210 patients. Results EDSS worsening was associated with higher baseline LDL (p = 0.006 and total cholesterol (p = 0.001, 0.008 levels, with trends for higher triglyceride (p = 0.025; HDL was not associated. A similar pattern was found for MSSS worsening. Higher HDL levels (p p = 0.033. Conclusions Serum lipid profile has modest effects on disease progression in MS. Worsening disability is associated with higher levels of LDL, total cholesterol and triglycerides. Higher HDL is associated with lower levels of acute inflammatory activity.

  13. Association of serum testosterone with lipid abnormalities in patients with angiographically proven coronary artery disease

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    Chandima Madhu Wickramatilake

    2013-01-01

    Full Text Available Context: Low testosterone levels are associated with an atherogenic lipid profile and may contribute to the pathogenesis of atherosclerosis. Aims: Our study aimed to investigate the relationship between serum total testosterone (TT levels and lipid profile in angiographically confirmed coronary artery disease (CAD in men. Settings and Design: This is a case-control hospital-based study at Teaching Hospital, Karapitiya, Galle, Sri Lanka. Materials and Methods: Two hundred and six men, 103 with angiographically proven CAD and 103 healthy men as a control group were studied. The serum levels of TT and lipids were assessed. Statistical Analysis: Data were analyzed using Minitab software (version 15 for Windows. Results: The mean concentrations of lipid parameters of patients and controls were as follows: Serum total cholesterol (TCh, 5.9 ± 2.8 vs. 5.2 ± 1.6 mmol/l (P = 0.022, low-density lipoprotein cholesterol (LDL-Ch, 3.9 ± 1.2 vs. 3.1 ± 0.5 mmol/l (P = 0.001, high-density lipoprotein cholesterol (HDL-Ch, 1.1 ± 0.5 vs. 1.4 ± 0.6 mmol/l (P = 0.001, and TGs, 2.0 ± 1.0 vs. 1.5 ± 0.8 mmol/l (P = 0.001; lipid levels were significantly different between the two groups. The mean levels of TT in the patients and controls were 11.4 ± 2.7 vs. 18.1 ± 7.2 nmol/l (P = 0.001, significantly different. Among CAD patients, a significant positive association was found between testosterone and HDL-Ch (r = 0.623, P = 0.001, whereas a negative association was found with LDL-Ch (r = -0.579, P = 0.001. Conclusions: Low levels of TT in men with CAD that appear together with an atherogenic lipid milieu may be involved in the pathogenesis of CAD. The observed association between testosterone and HDL-Ch suggests a protective effect of the hormone.

  14. The correlation of the serum leptin, insulin resistance, endocrine hormone, blood lipid and of simple obesity children and growth

    Institute of Scientific and Technical Information of China (English)

    Xiao-Yu Wang; Xiao-Yun Liu; Kai-Yu Xu

    2015-01-01

    Objective:To analyze the correlation of the serum leptin, insulin resistance, Endocrine hormone, blood lipid and of simple obesity children and growth.Methods:200 cases of simple obesity children (Study Group) and 200 cases of non obese children (control group) were studied, Fasting blood glucose(FBG), Glycosylated hemoglobin (HbAlc), the serum leptin detection (Leptin), insulin (INS), insulin resistance index (HOMA2-IR), growth hormone (GH), cortisol (cortisol), thyroid hormones and blood lipid indexes were detected.Results:Leptin, cortisol and T3 levels of study group were (21.39±2.29) g/L, (18.43±1.72) g/mL and (20.71±1.82) pmol/L, were significantly increased compared with the control group, and GH level was (3.15±0.41) g/L, was significantly reduced, the difference of all these indexes were statistically significant in two groups (P0.05), HbAlc, INS and HOMA2 - IR were respectively (5.82±0.87)%, (33.02-2.34) mIU/L, (3.31-0.54), compared with the control group obviously increase than the corresponding numerical of the control group, the difference of all these indexes have statistical significance in two groups (P<0.05). TC, TG and LDL-C of study group were (4.67±0.65) mmol/L, (1.62±0.45) mmol/L and (3.59±0.46) mmol/L, were significantly higher than those in the control group, while HLD-C was (1.10±0.24) mmol/L, was significantly lower (P<0.05). Conclusion:Children with simple obesity have leptin resistance, reduction of growth hormone and endocrine and lipid metabolism disorder, and seriously affect the normal growth and development of children.

  15. Serum Lipids and Diabetic Retinopathy in Newly Diagnosed Type 2 Diabetic Subjects

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    Shahana Shermin

    2011-07-01

    Full Text Available Background: Diabetic retinopathy is the commonest and usually the first observable vascular complication of diabetes mellitus. Along with hyperglycaemia, dyslipidaemia is a contributing factor for the occurrence of diabetic retinopathy. It is postulated that dyslipidaemia results in formation of hard exudate by increasing blood viscosity and altering the fibrinolytic system. A case control study was carried out in the department of Biochemistry, Bangabandhu Sheikh Mujib Medical University, Dhaka during the period of January 2006 to December 2007 to evaluate the serum lipid profile in newly diagnosed type 2 diabetic subjects with diabetic retinopathy. Materials and Methods: Total 85 newly diagnosed type 2 diabetic subjects were included in this study, 40 were cases having retinopathy and 45 were age and sex matched controls without retinopathy. Serum triglyceride (TG, total cholesterol (TC, low density lipoprotein cholesterol (LDL-C and high density lipoprotein cholesterol (HDL-C were compared between cases and controls. Unpaired t-test and chi-square test were done between groups as tests of significance. Results: All the parameters of lipid profile showed dyslipidaemic trend both in cases and controls. In the cases TG was significantly higher and HDL-C was significantly lower than that of controls (p < 0.05 whereas no significant difference was found between cases and controls with respect to serum TC and LDL-C. Conclusion: It can be concluded that high TG and low HDL-C are associated with diabetic retinopathy in newly diagnosed type 2 diabetes.

  16. Clinical significance of determination of plasma NPY levels and serum lipid profile in patients with cerebral hemorrhage and cerebral infarction

    International Nuclear Information System (INIS)

    Objective: To study the clinical significance of changes of plasma NPY levels and serum lipid profile in patients with cerebral hemorrhage and cerebral infarction. Methods: Plasma NPY levels (with RIA) and serum lipid profile (with biochemistry) were determined in (1) 48 patients with acute cerebral hemorrhage (2) 46 patients with acute cerebral infarction and (3) controls.Results Plasma NPY levels in both patients with cerebral hemorrhage and patients with cerebral infarction were significantly higher than those in controls (P0.05). Conclusion: NPY played important roles in the development and pathogenesis of cerebral vascular accidents. Lipid profile changes was the basic etiological factor. (authors)

  17. Association of the KLF14 rs4731702 SNP and serum lipid levels in the Guangxi Mulao and Han populations.

    Science.gov (United States)

    Huang, Ping; Yin, Rui-Xing; Huang, Ke-Ke; Zeng, Xiao-Na; Guo, Tao; Lin, Quan-Zhen; Wu, Jian; Wu, Dong-Feng; Li, Hui; Pan, Shang-Ling

    2013-01-01

    The objective of the present study was to detect the association of the rs4731702 single nucleotide polymorphism (SNP) and serum lipid levels in the Guangxi Mulao and Han populations. A total of 727 subjects of Mulao and 740 subjects of Han Chinese were included. Serum low-density lipoprotein cholesterol (LDL-C) and apolipoprotein (Apo) B levels were higher in Mulao than in Han (P KLF14 rs4731702 SNP and serum lipid levels between the two ethnic groups might partly result from different gene-environmental interactions. PMID:24195066

  18. Maternal Serum Lipid, Estradiol, and Progesterone Levels in Pregnancy, and the Impact of Placental and Hepatic Pathologies

    Science.gov (United States)

    Pecks, U.; Rath, W.; Kleine-Eggebrecht, N.; Maass, N.; Voigt, F.; Goecke, T. W.; Mohaupt, M. G.; Escher, G.

    2016-01-01

    Objective: Lipids and steroid hormones are closely linked. While cholesterol is the substrate for (placental) steroid hormone synthesis, steroid hormones regulate hepatic lipid production. The aim of this study was to quantify circulating steroid hormones and lipid metabolites, and to characterize their interactions in normal and pathological pregnancies with a focus on hepatic and placental pathologies. Methods: A total of 216 serum samples were analyzed. Group A consisted of 32 patients with uncomplicated pregnancies who were analyzed at three different time-points in pregnancy (from the first through the third trimester) and once post partum. Group B consisted of 36 patients (24th to 42nd week of gestation) with pregnancy pathologies (IUGR n = 10, preeclampsia n = 13, HELLP n = 6, intrahepatic cholestasis n = 7) and 31 controls with uncomplicated pregnancies. Steroid profiles including estradiol, progesterone, and dehydroepiandrosterone were measured by GC-MS and compared with lipid concentrations. Results: In Group A, cholesterol and triglycerides correlated positively with estradiol (cholesterol ρ = 0.50, triglycerides ρ = 0.57) and progesterone (ρ = 0.49, ρ = 0.53) and negatively with dehydroepiandrosterone (ρ = − 0.47, ρ = − 0.38). Smoking during pregnancy affected estradiol concentrations, leading to lower levels in the third trimester compared to non-smoking patients (p < 0.05). In Group B, cholesterol levels were found to be lower in IUGR pregnancies and in patients with HELLP syndrome compared to controls (p < 0.05). Steroid hormone concentrations of estradiol (p < 0.05) and progesterone (p < 0.01) were lower in pregnancies with IUGR. Discussion: Lipid and steroid levels were affected most in IUGR pregnancies, while only minor changes in concentrations were observed for other pregnancy-related disorders. Each of the analyzed entities displayed specific changes. However, since the

  19. Efficacy of Tribulus Terrestris Extract on the Serum Glucose and Lipids of Women with Diabetes Mellitus

    Science.gov (United States)

    Samani, Nasrin Babadai; Jokar, Azam; Soveid, Mahmood; Heydari, Mojtaba; Mosavat, Seyed Hamdollah

    2016-01-01

    Background: Considering folkloric use of Tribulus terrestris (T. terrestris) in diabetes and proven anti-hyperglycemic and anti-hyperlipidemic effects of T. terrestris in animal studies, we aimed to evaluate the efficacy of the hydro alcoholic extract of T. terrestris on the serum glucose and lipid profile of women with diabetes mellitus. Methods: Ninety-eight diabetic women were randomly allocated to receive the T. terrestris (1000 mg/day) or placebo for three months. The patients were evaluated in terms of the fasting blood glucose, 2-hour postprandial glucose, glycosylated hemoglobin and lipid profile. Results: T. terrestris showed a significant blood glucose lowering effect in diabetic women compared to placebo (Pdiabetic women.

  20. Analysis of the changes of serum lipid and hs-CRP in patients with subclinical hypothyroidism

    Institute of Scientific and Technical Information of China (English)

    Jin-Mian Fan; Dong-Bo Zheng; Zhou-Ping Zhang

    2016-01-01

    Objective:To analyze changes of serum lipid and hs-CRP levels in patients with subclinical hypothyroidism in order to explore their correlations with TSH level.Methods:A total of 90 patients with subclinical hypothyroidism were included in the study and divided into the mild, moderate, and severe groups according to TSH level with 30 cases in each group; meanwhile, 30 healthy individuals were served as the control groups. The changes of the levels of TSH, serum lipid indicators (TC, TG, HDL-C, LDL-C, ApoA, ApoB, and Lpa), and hs-CRP in the four groups were observed. The correlation of serum lipid and hs-CRP with TSH was analyzed. Results: The levels of TSH, TC, TG, Lpa, and hs-CRP in the subclinical hypothyroidism subgroups were significantly higher than those in the control group. The levels of HDL-C, LDL-C, ApoA, and ApoB in patients with subclinical hypothyroidism were increased when compared with the control group. The levels of HDL-C, LDL-C, and ApoB in the moderate and severe groups were significantly higher than those in the control group. ApoA level in the severe group was significantly higher than that in the control group. LDL-C in the moderate group was significantly higher than that in the mild group, and that in the severe group was significantly higher than that in the moderate group. ApoB level in the severe group was significantly higher than that in the mild group. Spearman correlation analysis showed that TC, TG, LDL-C, ApoB, Lpa, and hs-CRP were positively correlated with TSH level, while HDL-C and ApoA had no correlation with TSH level.Conclusions:The levels of serum lipid and hs-CRP are abnormally elevated in patients with subclinical hypothyroidism, and are gradually increased along with the disease progression, which is closely associated with TSH level.

  1. Serum lipids, apoproteins and nutrient intake in rural Cretan boys consuming high-olive-oil diets

    OpenAIRE

    Aravanis, C; Mensink, R.P.; Karalias, N.; Christodoulou, B.; Kafatos, A.; Katan, M.B.

    1988-01-01

    A high intake of olive oil has produced high levels of high-density and low levels of low-density lipoprotein cholesterol in short-term dietary trials. To investigate long-term effects of olive oil we have studied the diet and serum lipids of boys in Crete, where a high olive oil consumption is the norm. Seventy-six healthy rural Cretan boys aged 7–9 years were studied. The diet was assessed by a 2-day dietary recall. Blood was collected according to a standardized protocol and sera were anal...

  2. Interaction of lipid vesicle with silver nanoparticle-serum albumin protein corona

    Science.gov (United States)

    Chen, Ran; Choudhary, Poonam; Schurr, Ryan N.; Bhattacharya, Priyanka; Brown, Jared M.; Chun Ke, Pu

    2012-01-01

    The physical interaction between a lipid vesicle and a silver nanoparticle (AgNP)-human serum albumin (HSA) protein "corona" has been examined. Specifically, the binding of AgNPs and HSA was analyzed by spectrophotometry, and the induced conformational changes of the HSA were inferred from circular dichroism spectroscopy. The fluidity of the vesicle, a model system for mimicking cell membrane, was found to increase with the increased exposure to AgNP-HSA corona, though less pronounced compared to that induced by AgNPs alone. This study offers additional information for understanding the role of physical forces in nanoparticle-cell interaction and has implications for nanomedicine and nanotoxicology.

  3. Effects of Headgroups and Serum on Gene Transfection of Alkaline Amino Acid Based Cationic Lipids

    Institute of Scientific and Technical Information of China (English)

    LI Li; YANG Yang; NIE Yu; HE Bin; GU Zhong-wei

    2009-01-01

    Three cationic lipids with lysylated(l), histidylated(2), and arginylated(3) headgroups and cholesterol hy-drophobic moiety were synthesized. The average sizes of liposomes and lipoplexes were around 100 and 160 nm, re-spectively. The gene transfection efficiency of the three lipoplexes loaded with pGL3 or pORF-LacZ was compared on 293T cells in the presence or the absence of serum. The transfection efficiency of the three lipoplexes in a se-rum-free medium was 2 to 3-fold higher than that of dioleoyl-trimethylammonium propane(DOTAP). In the presence of serum, however, most of the lipoplexes showed lower transfection activities; only lipoplex 3 retained its high transfection efficiency.

  4. Gender and Age Impact on the Association Between Thyroid-Stimulating Hormone and Serum Lipids.

    Science.gov (United States)

    Meng, Zhaowei; Liu, Ming; Zhang, Qing; Liu, Li; Song, Kun; Tan, Jian; Jia, Qiang; Zhang, Guizhi; Wang, Renfei; He, Yajing; Ren, Xiaojun; Zhu, Mei; He, Qing; Wang, Shen; Li, Xue; Zheng, Wei; Hu, Tianpeng; Liu, Na; Upadhyaya, Arun; Zhou, Pingping; Zhang, Jianping

    2015-12-01

    The relationship between thyroid-stimulating hormone (TSH) and hyperlipidemia is still a topic of debate. We aimed to explore the impact of gender and age on the association between serum TSH and lipid profile in a large cohort of Chinese.This cross-sectional study enrolled 13,915 participants (8565 male, 5350 female), who self-reported as healthy without any known previous diseases. Clinical data including anthropometric measurements, thyroid function, and other serum parameters were collected. The associations between TSH and hyperlipidemia of males and females were analyzed separately after dividing TSH and age into subgroups. Odds ratio for hyperlipidemia was calculated by binary logistic regression models.Young males had significantly higher prevalence of hypercholesterolemia, hypertriglyceridemia, and high serum low-density lipoprotein-cholesterol than females, yet after menopause, females had higher prevalence than males. TSH was positively associated with hyperlipidemia independent of thyroid hormones. Males showed more reduced risks of hyperlipidemia in low TSH concentrations, while females demonstrated more enhanced risks of hyperlipidemia in high TSH concentrations. For instance, if TSH was lower than 0.3 μIU/mL, the risks of developing hypercholesterolemia and hypertriglyceridemia in males were only 0.198 (P  0.05), respectively. If TSH was higher than 4.0 μIU/mL, women displayed significantly higher risks of developing hypertriglyceridemia than the reference TSH risks (P thyroid hormone independent positive associations between serum TSH and lipids, which were substantially influenced by gender and age. Males demonstrated more protective effects of low TSH against hyperlipidemia, while females showed more detrimental effects of high TSH on hyperlipidemia. PMID:26656346

  5. The Effect of Long-Term Carbamazepine Monotherapy on Serum Lipid Levels in Epilepsy Patients

    Directory of Open Access Journals (Sweden)

    İsmail Apak

    2008-01-01

    Full Text Available The aim of this study was to determine the effect of carbamazepine on serum lipid levels in epileptic patients who were on long-term carbamazepine monotherapy. The study group were comprised of 30 epileptic patients (10 female, 20 male who have been on carbamazepine monotherapy for at least one year whereas control group consisted of 30 age and sex matched healthy controls. Serum cholesterol (total cholesterol, HDL cholesterol, LDL cholesterol and triglyceride levels were measured and LDL/HDL ratio was calculated in all subjects. Serum HDL cholesterol and triglyceride levels of study group were significantly higher than control group whereas serum LDL cholesterol levels and LDL/HDL ratios of study group were lower than control group. Mean total cholesterol level of study group was lower than control group, however the difference did not reach statistical significance level. Because of its effect on cholesterol levels, long term carbamazepine could possibly have a positive influence in decreasing the risk of developing aterosclerosis and coronary heart disease. Long term prospective follow-up studies would be helpful to us in enlightening this issue definitely.

  6. Effect of alpha-linolenic acid-rich Camelina sativa oil on serum fatty acid composition and serum lipids in hypercholesterolemic subjects.

    Science.gov (United States)

    Karvonen, Henna M; Aro, Antti; Tapola, Niina S; Salminen, Irma; Uusitupa, Matti I j; Sarkkinen, Essi S

    2002-10-01

    Camelina sativa-derived oil (camelina oil) is a good source of alpha-linolenic acid. The proportion of alpha-linolenic acid in serum fatty acids is associated with the risk of cardiovascular diseases. We studied the effects of camelina oil on serum lipids and on the fatty acid composition of total lipids in comparison to rapeseed and olive oils in a parallel, double-blind setting. Sixty-eight hypercholesterolemic subjects aged 28 to 65 years were randomly assigned after a 2-week pretrial period to 1 of 3 oil groups: camelina oil, olive oil, and rapeseed oil. Subjects consumed daily 30 g (actual intake, approximately 33 mL) of test oils for 6 weeks. In the camelina group, the proportion of alpha-linolenic acid in fatty acids of serum lipids was significantly higher (P camelina group from those in other groups. During the intervention, the serum low-density lipoprotein (LDL) cholesterol concentration decreased significantly by 12.2% in the camelina oil group, 5.4% in the rapeseed oil group, and 7.7% in the olive oil group. In conclusion, camelina oil significantly elevated the proportions of alpha-linolenic acid and its metabolites in serum of mildly or moderately hypercholesterolemic subjects. Camelina oil's serum cholesterol-lowering effect was comparable to that of rapeseed and olive oils. PMID:12370843

  7. Effects of Dietary Strawberry Supplementation on Antioxidant Biomarkers in Obese Adults with Above Optimal Serum Lipids

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    Arpita Basu

    2016-01-01

    Full Text Available Berries have shown several cardiovascular health benefits and have been associated with antioxidant functions in experimental models. Clinical studies are limited. We examined the antioxidant effects of freeze-dried strawberries (FDS in adults [n=60; age: 49±10 years; BMI: 36±5 kg/m2 (mean ± SD] with abdominal adiposity and elevated serum lipids. Participants were randomized to one of the following arms: low dose strawberry (25 g/day FDS, low dose control beverage (LD-C, high dose strawberry (50 g/d FDS, and high dose control beverage (HD-C for 12 weeks. Control beverages were matched for calories and total fiber. Plasma antioxidant capacity, trace elements (copper, iron, selenium, and zinc, whole blood glutathione (GSH, and enzyme activity (catalase, glutathione peroxidase, and glutathione reductase were examined at screening (0 week and after 12 weeks’ intervention. At 12 weeks, plasma antioxidant capacity and glutathione levels were higher in the strawberry versus control groups (low and high dose FDS: 45% and 42% for plasma antioxidant capacity and 28% and 36% for glutathione, resp.; glutathione was higher in the high versus low dose strawberry group (all p<0.05. Serum catalase activity was higher in the low dose strawberry (43% versus control group (p<0.01. No differences were noted in plasma trace elements and glutathione enzyme activity. Dietary strawberries may selectively increase plasma antioxidant biomarkers in obese adults with elevated lipids.

  8. GUAR GUM EFFECTS ON BLOOD SERUM LIPIDS AND GLUCOSE CONCENTRATIONS OF WISTAR DIABETIC RATS1

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    DARIO FRIAS Andrea

    1998-01-01

    Full Text Available The effects of guar gum derived from the endosperm of Cyamopsis tetragonoloba (75% soluble fiber, 7.6% insoluble fiber, 2.16% crude protein, 0.78% total lipids, 0.54% ash and 9.55% moisture on diabetic rats were studied concerning food intake, body weight gain, blood serum cholesterol, triacylglycerols, glucose, LDL-, and HDL-cholesterol concentrations. The effect of gum on indexes of protein absorption and utilization was also investigated. Diets containing 0%, 10% and 20% (w/w guar gum were fed to diabetic rats for 28 days. In spite of the fact that diabetes elevated blood lipids in all animals, guar gum diet significantly decreased (p <0.05 serum concentrations of cholesterol and triacylglycerols. Furthermore, a concomitant increase in HDL-cholesterol with a substancial elevation of the HDL/LDL cholesterol ratio was found. The most significant result in this assay was the drastic reduction of blood glucose in diabetic rats treated with guar gum diet. The gum promoted a general improvement in the condition of the diabetic rats, in body weight gain and indexes of protein absorption and utilization. The results of this research suggest that guar gum, at concentrations equal to or higher than 10%, should be effective in the treatment of hypercholesterolemia and diabetes, in humans.

  9. Effects of Regular Recreational Exercise Training on Serum ANGPTL3-Like Protein and Lipid Profile in Young Healthy Adults

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    Smol Ewelina

    2015-12-01

    Full Text Available Evidence of the role of ANGPTL3, a liver-secreted glycoprotein, in serum lipid turnover, led us to hypothesize that this protein may be involved in modification of the lipid profile induced by exercise-training. Given the lack of data regarding this issue, the main goal of the present study was to investigate the effects of regular participation in a recreational physical activity program on serum ANGPTL3 and selected lipid profile measures in young, apparently healthy female and male adults. We compared serum ANGPTL3, lipid profile measures, common lipid ratios, the Atherogenic Index of Plasma (AIP and glucose in fasting blood samples derived from 22 active physical education students including active females (AF, N=6 and males (AM, N=16 with samples from 28 relatively sedentary agematched peers, including female (SF, N=9 and male (SM, N=19 individuals not involved in any regular physical conditioning program. Despite high inter-individual variability of serum ANGPTL3, there was a general tendency toward higher serum ANGPTL3 and HDL-C in women compared to men, but without significant differences related to their physical activity status. Based on both routine lipid profile measures and lipid ratios, all participants had normal lipid profiles, normal glycemia, as well as favorable anthropometric indices not suggesting increased cardiometabolic risk. However, lower levels of the TG/HDL-C ratio and AIP in physically active compared to relatively sedentary participants, reflecting the predominance of large, buoyant LDL particles, strongly support the view of beneficial healthpromoting effects of regular participation in recreational sport activities.

  10. EFFECT OF CINNAMON AND GINGER COMPARED TO DOXYSTIN (ANTIMICROBIAL DRUG ON SERUM LIPID PROFILE IN BROILER CHICKS

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    ELBAGIR M. NABIELA

    2013-01-01

    Full Text Available The aim of this study was to assess the effect of the medicinal plants cinnamon (Cinnamomum verum and ginger (Zingiber officinale, as natural feed additives in comparison to (Doxystin “Doxycycline HCl 50 mg and Colistin sulfate” , known antimicrobial growth promoter on the serum lipid profile of broiler chicks. One hundred and sixty (one day-old broiler chicks were assigned to four groups of the same mean weight, each with four replicates of ten chicks. The first group was used as control group and fed broilers basal diet, the second group fed the basal diet supplemented with the (Doxystin as 0.5%, the third and fourth groups fed basal diet mixed with C. verum, and Z. officinale as 2% of the diet respectively. The experimental diets affected all parameters measured follows, total cholesterol and serum (low density lipoprotein LDL-C concentration was significantly (P<0.05 decreased in groups received spices diet compared to Doxystin and control groups. Whereas, the (high density lipoprotein HDL-C concentration showed significantly (P<0.05 lower levels in the two spice treated groups compared to the control group only, and the antibiotic treated animals showed similar level to that observed in spice treated groups. Triacylglycerols and the VLDL-C fraction showed clearly reduced values in all treated groups compared to the control group, though the difference was not significant but it was more pronounced in the spice treated groups, as they reported half the level of the control group. It can be concluded that inclusion of C. verum and Z. officinale as feed additives acted as natural hypocholesterolemic agents in broiler chicks in particular and reduced blood lipids in general.

  11. Assessment of serum lipids in patients with age related macular degeneration from Pakistan

    International Nuclear Information System (INIS)

    Objective: To determine serum lipids in patients with age related macular degeneration from Pakistani population. Methods: The study was a cross sectional, randomized and case-control. Selected subjects ages were >50 years and were normotensive, non-diabetic with no family history of any such disease and no complication of posterior ocular chamber other than age related macular degeneration (AMD). Controls were age matched healthy individuals with no symptoms of AMD. Diagnosis of AMD was done through conventional diagnostic techniques by professional ophthalmologists. Serum samples were analyzed for total cholesterol, triglycerides, LDL and HDL using commercially available kits. Data were compared with Student's t-test. Pearson correlation was calculated for relationship between different parameters. P<0.05 was considered significant. Results: Compared to controls, AMD patients had significantly greater total cholesterol concentration (p<0.041), and power HDL/LDL ratio (p<0.038), while serum triglycerides, HDL and LDL were non-significantly different from control subjects. Total cholesterol in AMD patients was significantly correlated with TG, LDL and HDL (p<0.0001). Conclusion: The study indicates that high cholesterol might be a predictor of AMD and can be a diagnostic parameter. (author)

  12. Assessment of serum lipids in nigerians with type-2 diabetes mellitus complications

    International Nuclear Information System (INIS)

    To assess the serum lipids and lipoprotein cholesterol in patients with complicated type 2 Diabetes Mellitus (DM): Hypertensive diabetics and diabetic nephropathy. This is a cross-sectional study. A total of 52 type 2 DM patients and 20 healthy controls were studied. The patients' population consisted of 23 normotensive diabetics, 16 hypertensive diabetics and 13 patients with diabetic nephropathy. The serum total cholesterol, HDL-cholesterol and triglycerides were assayed in patients and controls, using standardized assay methods. The mean serum total cholesterol was higher in patients than controls. The normotensive diabetic patients had the lowest total cholesterol among the patients groups 4.01+-0.82 mmol/L compared to the hypertensive diabetics 6.01+-0.93 mmol/L and the diabetic nephropathy patients 6.90+-1.20 mmol/L, (P <0.0001). The prevalence of dyslipidaemia in the patients was between 25% - 69%, lowest in the normotensive diabetics and highest in the diabetic nephropathy patients. We hope that these findings will draw specific attention to the management of dyslipidaemia in patients with complicated type 2 DM especially diabetic nephropathy and hypertensive diabetics. (author)

  13. [Heritability analysis on serum lipids of adult twins in Qingdao City

    DEFF Research Database (Denmark)

    Lan, Jinfeng; Pang, Zengchang; Wang, Shaojie;

    2010-01-01

    OBJECTIVE: To study the level and heritability of serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and triglycerides (TG) in adult twins sampled from Qingdao City of China. METHODS: 316 pairs of healthy twin aged 18 to 60 years...... were recruited from the database of Qingdao City twin registry. Fasting serum lipids were detected by automatic biochemical analyzer. The zygosity of twins was established by using polymorphic DNA-based microsatellite markers. The heritability was estimated by formulating univariate ACE twin mode in Mx...... soft. RESULTS: The average levels of fasting serum TC, HDL-C, LDL-C and TG were (4.52 +/- 1.08), (1.46 +/- 0.39), (2.58 +/- 0.79) and (1.11 +/- 0.77) mmol/L, respectively. The best fitting models adjusted by sex and age in Mx soft were AE model for TC, HDL-C and LDL-C, whereas CE model for TG. The...

  14. The association of serum lipids with the histological pattern of rectosigmoid adenoma in Taiwanese adults

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    Lu Feng-Hwa

    2011-05-01

    Full Text Available Abstract Background The mortality rate of colorectal cancer ranks third behind lung and hepatic cancer in Taiwan. Colorectal cancer mostly arises from adenomatous polyps of left colon. The aim of our study was to examine the association of serum lipids with the histological pattern of rectosigmoid adenoma. Methods There were 2,506 eligible examinees aged 20 and above who underwent sigmoidoscopy as a screening examination in National Cheng Kung University Hospital between January 2003 and October 2006. They were classified into three groups: tubular adenoma (333 subjects, villous-rich (tubulovillous/villous adenoma (53 subjects and normal (2,120 subjects. We defined high total cholesterol (TC as a level ≧200 mg/dl, low high-density lipoprotein cholesterol (HDL-C as a level Results Among the study population, 333 subjects (13.3% had tubular adenomas and 53 subjects (2.1% had villous-rich adenomas. The odds ratio (OR for villous-rich adenoma in subjects with TG≧200 mg/dL compared to those with TG Conclusions Our study showed that subjects with high serum TG tended to have a higher risk of tubulovillous/villous adenoma in rectosigmoid colon. Therefore, reducing the serum TG level might be one method to prevent the incidence of colorectal cancer.

  15. Investigation of the roles of the substances in serum lipids and their constitutive fatty acids in chronic urticaria.

    Science.gov (United States)

    Kobayashi, S

    1989-06-01

    The newly-generated lipid mediators include products of arachidonate metabolism, prostaglandins and leukotrienes. In this study, serum lipids and fatty acids, including arachidonic acid (C20:4) were examined in 12 normal subjects (6 males and 6 females) and 23 subjects with chronic urticaria (6 males and 17 females), including 17 who made an excellent or good recovery (4 males and 13 females). The results indicated a relationship between chronic urticaria and serum lipids and fatty acids. The omega 6 (n-6) and omega 3 (n-3) series of polyunsaturated fatty acids and lipid peroxidation were suggested that may be one of the mediators in chronic urticaria. Pantethine, glutathione and ascorbic acid were effective in controlling chronic urticaria. PMID:2794222

  16. Serum lipid profile in alcoholic cirrhosis: A study in a teaching hospital of north-eastern India

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    Jyoti Prakash Phukan

    2013-01-01

    Full Text Available Background: Alcoholic cirrhosis is often associated with impaired lipid metabolism. However, there are only a few studies regarding lipid profile in alcoholic cirrhosis that have been undertaken in India. The aim of the study is to assess the degree of alteration of serum lipid profile in alcoholic cirrhotic patients and also to detect its relationship with the age of the patients and the alcohol consumption pattern. Patients and Methods: This cross-sectional study was conducted in a teaching hospital of north-eastern India for 1 year with 100 patients with alcoholic cirrhosis (cases and 50 healthy individuals (controls without history of alcohol consumption. A questionnaire of personal characteristics including history of alcoholism was completed for each patient. Serum lipid profile (total, low-density lipoprotein (LDL, high-density lipoprotein (HDL cholesterol and triglyceride was recorded for each case and control. t test of significance was applied for statistical analysis. Results: Majority of the cases were in the 41-50 years age group. There was no relationship of cirrhosis with the type of alcoholic beverage, but a definite relationship was observed with the quantity and the duration of alcohol consumption. In patients with cirrhosis, the total serum cholesterol level was decreased. There was a significant decrease in serum HDL and LDL cholesterol compared with the control group ( P < 0.001. However, the serum triglyceride levels were significantly increased in alcoholic cirrhotic patients compared with the control group ( P < 0.001. Conclusion: In this study, we found that there was marked alteration of serum lipid profile values in patients with alcoholic cirrhosis compared with normal, non-cirrhotic individuals. Therefore, a search for lipid profile abnormality should be performed in every cirrhotic patient.

  17. Effects of Combined Selective Aerobic Moderate Intensity Exercises and Soya Intake on Serum Lipids and Obesity in Obese Postmenopausal Women

    OpenAIRE

    A Zarneshan

    2012-01-01

    Background & Aim: Today, the percentage of deaths due to heart diseases has increased and along with the exercise training role in reducing obesity and cardiovascular disease, soy is a useful source of food in reducing blood lipid and obesity. The present study investigated the effect of combined selective short aerobic moderate intensity exercise and soya intake on serum lipids and obesity in obese postmenopausal women. Methods: In the present quasi-experimental study conducted in 2006 o...

  18. Determination of RBC membrane and serum lipid composition in trinidadian type II diabetics with and without nephropathy

    OpenAIRE

    Nayak, B Shivananda; Vishi Y Beharry; Armoogam, Shivani; Nancoo, Melinda; Ramadhin, Kevin; Ramesar, Kiron; Ramnarine, Ciara; Singh, Anandi; Singh, Anisha; Nwachi, Kingsley Uche; Teelucksing, Surujpaul; Mathura, Ramesh; Roberts, Lesley

    2008-01-01

    Aim: The rheological properties of erythrocytes are impaired in diabetes mellitus, especially because of changes in their membrane lipid composition.The aim of this study was to determine and examine the relationship between red blood cell (RBC) membrane and serum lipid composition in type II diabetes subjects with and without nephropathy. Methods: Trinidadian subjects aged 18–65 years were recruited for the study regardless of gender and ethnicity. Fasting blood samples were collected from 6...

  19. Effects of cranberry powder on serum lipid profiles and biomarkers of oxidative stress in rats fed an atherogenic diet

    OpenAIRE

    Kim, Mi Joung; Jung, Ha Na; Kim, Ki Nam; Kwak, Ho-Kyung

    2008-01-01

    This study investigated that the antioxidative effect of freeze-dried cranberry powder against protein and lipid oxidation and ameliorative effect of serum lipid profile in rat fed atherogenic diet. Six weeks old male Sprague-Dawley rats were divided into the following four groups: normal diet group with 5% corn oil (control), atherogenic diet group with 5% corn oil, 10% lard, 1% cholesterol, and 0.5% sodium cholate (HFC), atherogenic plus 2% cranberry powder diet group (HFC + C2), and athero...

  20. Effects of Glycyrrhizic Acid on Peroxisome Proliferator-Activated Receptor Gamma (PPARγ, Lipoprotein Lipase (LPL, Serum Lipid and HOMA-IR in Rats

    Directory of Open Access Journals (Sweden)

    Chia Yoke Yin

    2010-01-01

    Full Text Available Studies on ligand binding potential of glycyrrhizic acid, a potential agonist to PPARγ, displayed encouraging results in amelioration of metabolic syndrome. The regulation of gene cassettes by PPARγ affects glucose homeostasis, lipid, lipoprotein metabolism and adipogenesis. This study was performed to determine the effects of GA on total PPARγ and LPL expression levels, lipid parameters and HOMA-IR. Oral administration of 100 mg/kg GA for 24 hours resulted in an increase in insulin sensitivity with decreases in blood glucose, serum insulin and HOMA-IR. Improvement in serum lipid parameters was also observed with a decrease in triacylglycerol, total cholesterol and LDL-cholesterol and an elevation in HDL-cholesterol. GA administration also resulted in up-regulation of total PPARγ and LPL expression levels in the visceral and subcutaneous adipose tissues, abdominal and quadriceps femoris muscles, as well as liver and kidney, with a significant up-regulation only in the visceral adipose tissue, abdominal and quadriceps femoris muscles. Thus, oral administration of 100 mg/kg GA for 24 hours improved insulin sensitivity and lipid profiles and induced upregulation of total PPARγ and LPL expression levels in all studied tissues.

  1. Association of the SPT2 chromatin protein domain containing 1 gene rs17579600 polymorphism and serum lipid traits

    OpenAIRE

    Guo, Tao; Yin, Rui-Xing; Bin, Yuan; Nie, Rong-Jun; Chen, Xia; Pan, Shang-Ling

    2015-01-01

    SPT2 chromatin protein domain containing 1 gene (SPTY2D1) is a candidate gene for dyslipidemia. The single nucleotide polymorphism (SNP) of rs7934205 near SPTY2D1 locus was ethnic- and sex-specific associated with serum lipid levels in our previous study. Whether SPTY2D1 rs17579600 SNP and several environmental factors are associated with serum lipid profiles is unknown. A total of 712 participants of Han and 689 unrelated individuals of Mulao were included. The genotype and allele frequencie...

  2. A CORRELATIVE STUDY ON SERUM LIPID PROFILE AND OSTEOGENIC MINERAL STATUS IN OSTEOPOROSIS

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    Deepika

    2016-03-01

    Full Text Available BACKGROUND Osteoporosis is a condition where the structural and functional integrity of the bones are altered to an extent where the bone material is not sufficiently completed enough to perform its basic function of support, load bearing and component of metabolic pool of osteogenic minerals. METHODS The study was conducted in a group of 50 women (25 cases and 25 controls irrespective of age from different socio-economic status. As per plan of study, the target population was divided into two main groups. The first group was defined as the control group and the other group as the experimental or test group who were admitted in orthopaedic unit of Gauhati Medical College and Hospital with different type of clinical manifestations of osteoporotic disease, the diagnosis of which were made on the basis of x-ray findings, provided the clinical examination and laboratory procedures are in agreement. RESULTS In our study, the mean fasting serum TG, HDL and VLDL values are significantly high (P<0.01 and the LDL value is significantly low in the women with osteoporosis in relation to normal control group. Total cholesterol values in osteoporotic group is apparently 6.07% lower than the mean total cholesterol in the normal control group. Osteogenic minerals represented by calcium, phosphate and magnesium show significant elevation (P<0.01 of mean serum concentration in the osteoporotic group than the normal control group. CONCLUSION Observed changes and relationship between the lipid profile and mineral status in serum in osteoporosis are indicative of an ongoing rearranging process in an altered metabolism, which is primarily aimed at diminishing its pace towards the associated complications indicated by attempted lowering of circulating cholesterol and maintenance of serum minerals within normal limits with assistance from a hormonal axis composed of components from metabolic-reproductive hormonal axis responsible for modulating natural aging process.

  3. Do bacterial vaginosis and chlamydial infection affect serum cytokine level?

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    Bogavac Mirjana

    2010-01-01

    Full Text Available Introduction. Serbia is the country with extremely low birth rate and a relatively high percentage of preterm deliveries (8%. With this in mind, discovering new diagnostic methods that could be used for the prediction of preterm delivery is of great importance. In this study we tried to determine whether bacterial vaginosis and chlamydial infection could provoke preterm delivery by activation of systemic cytokine network. Objective. The aim of this study was to determine serum levels of proinflammatory cytokines (IL-1β, IL-8, IFN-γ, IL-6 and TNF-α in pregnant women with symptoms of preterm delivery and to make correlation between these parameters and the presence of bacterial vaginosis or chlamydial infection. Method. In the serum of 35 pregnant women, which were divided in groups according to the presence or absence of bacterial vaginosis and chlamydial infection, commercial ELISA tests for proinflammatory cytokines were performed. Results. The serum level of IFN-γ was significantly increased in pregnant women having chlamydial infection, as well as the level of IL-1β in women with bacterial vaginosis. The levels of TNF-α, IL-6 and IL-8 were not significantly different between the investigated groups. Conclusion. The preliminary results obtained in this research point out the possibility that not only intrauterine or systemic infections, but also bacterial vaginosis and chlamydial infection can cause a partial activation of systemic cytokine network and contribute to the occurrence of preterm delivery.

  4. Relationship between Lipids Levels of Serum and Seminal Plasma and Semen Parameters in 631 Chinese Subfertile Men.

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    Jin-Chun Lu

    Full Text Available This prospective study was designed to investigate the relationship between lipids levels in both serum and seminal plasma and semen parameters.631 subfertile men were enrolled. Their obesity-associated markers were measured, and semen parameters were analyzed. Also, seminal plasma and serum TC, TG, HDL and LDL and serum FFA, FSH, LH, total testosterone (TT, estradiol (E2 and SHBG levels were detected.Seminal plasma and serum TG, TC and LDL levels were positively related to age. Serum TC, TG and LDL were positively related to obesity-associated markers (P < 0.001, while only seminal plasma TG was positively related to them (P < 0.05. For lipids levels in serum and seminal plasma, only TG level had slightly positive correlation between them (r = 0.081, P = 0.042. There was no significant correlation between serum lipids levels and semen parameters. However, seminal plasma TG, TC, LDL and HDL levels were negatively related to one or several semen parameters, including semen volume (SV, sperm concentration (SC, total sperm count (TSC, sperm motility, progressive motility (PR and total normal-progressively motile sperm counts (TNPMS. Moreover, seminal plasma TG, TC, LDL and HDL levels in patients with oligospermatism, asthenospermia and teratozoospermia were higher than those with normal sperm concentration, motility or morphology. After adjusting age and serum LH, FSH, TT, E2 and SHBG levels, linear regression analysis showed that SV was still significantly correlated with seminal plasma LDL (P = 0.012, both of SC and TSC with seminal plasma HDL (P = 0.028 and 0.002, and both of PR and sperm motility with seminal plasma TC (P = 0.012 and 0.051.The abnormal metabolism of lipids in male reproductive system may contribute to male factor infertility.

  5. The Effects of Olive and Pumpkin Seed Oil on Serum Lipid Concentrations

    OpenAIRE

    Landeka, Irena; Đikić, Domagoj; Radišić, Iva; Teparić, Renata; Bačun-Družina, Višnja; Rogić, Dunja

    2011-01-01

    Low-fat diets increase serum triacilglycerol and decrease HDL-cholesterol concentrations, thereby potentially adversely affecting cardiovascular disease (CVD) risk. The present study compared the CVD risk profi le of a high-MUFA diets (olive oil) and high-PUFA diets (pumpkin seed oil). The most signifi cant difference between the two groups was that the atherogenic index in groups given olive oil was significantly lower (for approximately 60 %) than atherogenic index recorded in control group...

  6. [Effects of high- and low-intensity training on cardiorespiratory system and serum lipids].

    Science.gov (United States)

    Yanagibori, R; Aoki, K; Suzuki, Y; Gunji, A

    1991-08-01

    The differences between the effects of high- and low-intensity exercise training on cardiorespiratory system and serum lipid levels were examined under conditions in which the total work load was same. Twenty-two healthy, untrained female students, aged 18 to 24 years, participated in the 8-week training experiment. Two training groups exercised on bicycle ergometers 4-5d/wk for 8 wk with high-intensity (H: 70% VO2 max 13 min/session; N = 8) or in low-intensity (L: 36% VO2 max 33 min/session; N = 8). The control group (C: N = 6) continued its sedentary life style. Data of 6 students (H: N = 2, L: N = 2, C: N = 2) were excluded from the analysis, because they did not carry out their duties. The results of this study were as follows; 1) Aerobic capacity increased in both training groups. 2) After training, both heart rates and oxygen uptake during submaximal work loads significantly decreased in group H, but only heart rates significantly decreased in group L. 3) Heart rates with a same oxygen-uptake load during submaximal work seemed to increase in group H, but significantly decreased in group L. 4) The mechanical efficiency during the submaximal work load significantly increased in group H, but not in group L. 5) DBP and MBP during the submaximal work load in group L significantly decreased compared with those before the training. SBP with a same oxygen-uptake load during submaximal work increased in group H, but decreased in group L. 6) Rate pressure products both at rest and during submaximal exercise decreased significantly in group L. 7) Serum lipid levels remained in all groups. These results suggested that when the amount of exercise is the same, low-intensity exercise training with 35% VO2 max was more effective to increase endurance capacity and was safer than high-intensity exercise training. PMID:1956126

  7. The metabolic effects of di (2-ethyl hexyl phthalate medium dose on lipid profiles in serum and liver tissue

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    Buang Y

    2011-02-01

    Full Text Available Background: Di (2-ethyl hexyl phthalate is the most widely used  plasticizer in blood storage bag for transfusion. This substance can modify lipid metabolism. This study was aimed to elucidate the metabolic effects of di (2-ethyl hexyl phthalate medium dose on lipid profiles in serum and liver tissue.Methods: Sprague Dawley rats were fed 1.0 % di (2-ethyl hexyl phthalate diet (DEHP group, n=5 or a non-supplemented diet (control group, n=5 for 10 days. The rats were allowed to freely access each food. Serum lipid concentrations were measured using enzyme assay kits. Lipids of liver tissues were extracted and the lipid contents were determined. A peach of liver was prepared to determine the activities of malic enzyme and carnitine palmitoyl transferase-1 (CPT-1.Results: Serum lipid concentrations (mg/dL of DEHP group decreased compared to control (P<0.05. The serum triglyceride (TG concentrations of control and DEHP groups were respectively (100.5±16.5 and (31.2±1.7; phospholipid (PL, (143.3±7.8 and (88.9±3.2; total cholesterol, (88.7±4.6 and (51.9±2.3. The liver TG content of control and DEHP group (mg/g liver were respectively, (40.8±4.4 and (23.7±1.3; liver cholesterol were (3.36±0.29 and (2.33±0.23; and the liver PL were (36.5±1.0 and (41.7±0.6. Malic enzyme and CPT-1 activities (nmol/min/mg protein of DEHP group increased compared to control (P<0.05, in which their increases were approximately by 4.35- and 2.33-folds,  respectively.Conclusion: The di (2-ethyl hexyl phthalate medium dose attenuates lipids secretion from the liver cells into the bloodstream. The increase of liver PL level accompanied with the promotions of malic enzyme and the CPT-1 activities are the key factors of the dietary di (2-ethyl hexyl phthalate effects in rats to attenuate the lipid secretions from the livers. (Med J Indones 2011; 20:20-6Keywords: Di (2-ethyl hexyl phthalate, hyperphospholipids, lipolysis, liver lipids, serum lipids

  8. Association of the KLF14 rs4731702 SNP and Serum Lipid Levels in the Guangxi Mulao and Han Populations

    OpenAIRE

    Ping Huang; Rui-Xing Yin; Ke-Ke Huang; Xiao-Na Zeng; Tao Guo; Quan-Zhen Lin; Jian Wu; Dong-Feng Wu; Hui Li; Shang-Ling Pan

    2013-01-01

    The objective of the present study was to detect the association of the rs4731702 single nucleotide polymorphism (SNP) and serum lipid levels in the Guangxi Mulao and Han populations. A total of 727 subjects of Mulao and 740 subjects of Han Chinese were included. Serum low-density lipoprotein cholesterol (LDL-C) and apolipoprotein (Apo) B levels were higher in Mulao than in Han (P < 0.05). The T allele carriers had higher serum LDL-C and ApoAI levels in Mulao, whereas they had lower high-dens...

  9. ASSESSMENT OF SERUM LIPIDS IN TYPE II DIABETES MALE AND FEMALE PATIENTS

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    Annapurna

    2015-10-01

    Full Text Available BACKGROUND AND OBJECTIVE: Type II diabetes mellitus (T2DM often have both quantitative and qualitative abnormalities of lipoproteins that are responsible for increased incidence of microvascular and macrovascular complications. Incidence of coronary heart disease is three to four folds higher in patients with type 2 diabetes mellitus compared to non - diabetics. It has been proposed that the composition of lipid particles in diabetic dyslipidemia is more atherogenic than other types of dyslipidemia. This study was conducted to asses s serum lipid profile in type diabetes male and female patients. MATERIALS AND METHODS: This study was conducted on 50 T2DM patients with history more than ten yrs diabetes. Diabetic patients with overt complications and patients on drugs like diuretics, s teroids, oral contraceptives and beta blockers etc are excluded from study. 5 ml of whole blood was collected via vena puncture with the help of a disposable syringe in between 7.00am and 8.00am. Fasting plasma glucose and different Lipid fractions were es timated using standard procedure. All values were expressed as mean ± S.D. Statistical significance of differences between control and study groups were evaluated by student’s t test. A p - value less than 0.05 were considered as significant. RESULTS: The se rum total cholesterol, LDL cholesterol and triglycerides were significantly raised in both males and females . CONCLUSION: Hyperlipidaemia is a common finding among DM patients. DM patients should be screened and appropriate management should be instituted to reduce the risk of CHD and atherosclerosis

  10. Effect of Bougainvillea spectabilis Leaves on Serum Lipids in Albino Rats Fed with High Fat Diet

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    H. Saikia

    2011-04-01

    Full Text Available Dyslipidemia is major problem among population those have sedentary life style as well as in diabetics. Bougainvillea spectabilis is most commonly found in India as an ornamental plant and has got anti-diabetic action due to presence of one insulin mimicking agent D-pinitol (3-O-methyl-chiroinositol. The present investigation was undertaken to evaluate the effect of alcoholic extract of leaves of Bougainvillea spectabilis (AEBSL on serum lipid profile in albino rats fed with high fat diet (HFD and to compare it with a standard hypolipidaemic drug simvastatin. Thirty healthy albino rats of both sexes (100-200 g were randomized into 5 groups of 6 animals each. The groups were treated as follows: Group A: normal diet (ND; Group B: HFD (vanaspati ghee + coconut oil mixture in ratio of 3:2 at 10 ml/kg/day; Group C: HFD+ AEBSL (100 mg/kg/day; Group D: HFD + AEBSL (200 mg/kg/day; Group E: HFD + simvastatin (1.8 mg/kg/day. Lipid profile was estimated after 8 weeks of treatment. AEBSL showed a significant (p<0.01 reduction in total cholesterol (TC, Triglyceride (TG, Low density lipoprotein (LDL, Very low density lipoprotein (VLDL levels and significant (p<0.01 increase in high density lipoproteins (HDL in hypercholesteromic rats (Group C and Group D. AEBSL 200 mg/kg/day found to be more effective than AEBSL 100 mg/kg/day. There is also significant improvement in atherogenic index (p<0.01 and increases the percentage of protection AEBSL treated animals. Alcoholic extract of leaves of Bougainvillea spectabilis leaves have excellent lipid lowering potentiality.

  11. SERUM GAMMA-GLUTAMYL TRANSPEPTIDASE AND LIPIDS IN YOUNG ADULTS WITH UNCOMPLICATED ESSENTIAL HYPERTENSION

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    Rajarajeswari D, Ramalingam, Sharmila T, Prasad Naidu M, Naidu JN

    2015-07-01

    Full Text Available Introduction: Gamma-glutamyl transpeptidase initially used as an indicator of alcohol ingestion is now viewed as a sensitive marker of sub clinical inflammation. Recent clinical studies have shown its association with blood pressure and lipid profile. As GGT degrades glutathione, an antioxidant GGT can be considered as a pro inflammatory marker playing a role in atherogenesis and hypertension. Materials and methods: 65 subjects with Essential hypertension and 50 age and sex matched healthy controls both male and female between 18-50 years of age were recruited from General Medicine department of Narayana Medical college and Hospital, Nellore, A.P. Serum GGT was measured by calorimetric kinetic assay. Fasting Serum Triglycerides, Total Cholesterol and HDL cholesterol by standard enzymatic procedures and LDL cholesterol by Friedwald equation. Results: GGT is significantly elevated in hypertensive subjects (Mean±SD 64.2±18.62IU/L compared to controls (Mean±SD 26.20±8.91IU/L (P value <0.001. GGT is significantly correlated with systolic BP (r= 0.26 p<0.01 and diastolic BP(r= 0.28 p<0.01. Conclusion: Our findings suggest that elevated GGT in young adults may contribute to their susceptibility to hypertension and provide an additional evidence of novel role of GGT in cardiovascular risk evaluation.

  12. Study on the correlationship between serum leptin and lipids, insulin levels in patients with acute coronary syndrome (ACS)

    International Nuclear Information System (INIS)

    Objective: To explore the role of leptin in the pathogenesis of ACS through study on the correlationship between serum leptin and lipids, insulin levels in patients with ACS. Methods: Serum leptin, insulin (with RIA) and lipids (with biochemical method) levels were measured in 72 patients with coronary heart disease (ACS n=38, stable CHD n=34) and 36 controls. Results: Serum leptin, lipids and insulin levels were all significantly higher in the ACS patients than those in controls. Leptin levels were positively correlated with those of CH and TG (r=0.41, P<0.0; r=0.36, P<0.05); significantly positively correlated with those of insulin (r=0.58; P<0.01) and negatively correlated with those of HDL-C (r=-0.40, P<0.05). Conclusion: The higher serum leptin levels in patients with ACS were closely related to the derangement of lipid metabolism and increase of insulin resistance in metabolic syndrome. It could also accelerate the deposition of atheromatous plaque and initiate inflammation. (authors)

  13. Higher serum lipids and oxidative stress in patients with normal tension glaucoma, but not pseudoexfoliative glaucoma

    Science.gov (United States)

    Yilmaz, Necat; Coban, Deniz Turgut; Bayindir, Asli; Erol, Muhammet Kazim; Ellidag, Hamit Yasar; Giray, Ozlem; Sayrac, Suha; Tekeli, Seckin Ozgur; Eren, Esin

    2016-01-01

    This study entailed a cross-examination of oxidant/antioxidant balance, high-density lipoprotein (HDL)-linked paraoxonase 1 (PON1) phenotypes, and levels of serum routine lipids among patients with normal tension glaucoma (NTG) or pseudoexfoliative glaucoma (PEXG) compared with healthy control groups. We aimed to investigate the links between oxidative stress (OS), HDL-related antioxidant enzyme activities and dyslipidemia in distinct subtypes of glaucoma. The study included 32 patients with NTG, 31 patients with PEXG, and 40 control subjects. Levels of PON1 and arylesterase enzymatic activity, total oxidant status (TOS), and total antioxidant status were measured by spectrophotometry and OS indexes (OSI) were calculated. The phenotype distribution of PON1 was determined using the dual substrate method. Blood serum levels of HDL, low-density lipoprotein, total cholesterol (TC), and triglyceride (TG) were measured. The TOS and OSI values in the NTG group were significantly higher compared with the other groups (both p < 0.01). The phenotype distribution found in the glaucoma and control groups were NTG: QQ, 59.4%; QR, 37.5%; RR, 3.1%; PEXG: QQ, 45.1%; QR, 48.4%; RR, 6.5%; and in the control group: QQ, 42.5%; QR, 50.0%; RR, 7.5%. Serum TC levels were significantly higher than the control in both NTG and PEXG groups, whereas TG was significantly higher in NTG only (p < 0.01 and p < 0.02, respectively). Hyperlipidemia, OS and variations in phenotype distribution of PON1 may play a role in the pathogenesis of different types of glaucoma. PMID:26773174

  14. Effects of health food from cereal and nata de coco on serum lipids in human

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    Duangchan Hengsawadi

    2006-03-01

    Full Text Available The purpose of this study was to evaluate cereals and nata de coco supplementation on the lipid status in 22 subjects with hyperlipidemia. Subjects consisted of eleven men and eleven women aged 32-75 years, and had serum total cholesterol (TC level of >5.2 mmol/L, serum total triglyceride (TG level of >1.7 mmol/ L and low density lipoprotein-cholesterol (LDL-c level of >3.4 mmol/L. The study consisted of four weeks of control and 20 weeks of supplementation period. The subjects were given 15 g of the supplement twice daily for 20 weeks. The daily 30 g supplement consisted of unpolished rice, hulled mung bean, sweet corn, and nata de coco and provided 122.6 kcal, 5.5 g of protein, 0.5 g of fat, 24.1 g of carbohydrate and 2.7 g of dietary fiber. After 20 weeks, the subjects were divided into two groups, according to their dietary compliance, group A: > 90% compliance, and group B: < 90% compliance with the assigned supplement intake. There were 15 subjects in group A, and 7 in group B. Results showed that in group A the mean TG levels at weeks 4, 8, and 16 were significantly different (P<0.05, but no significant differences were observed in TC, LDL-c and HDL-c except that TC level at week 16 was significantly lower than that at week 0 (P<0.05. Thus, the results appeared to indicate that health food from unpolished rice, hulled mung bean, sweet corn and nata de coco may reduce the serum TG and TC in hyperlipidemic patients.

  15. Cardiovascular markers of inflammation and serum lipid levels in HIV-infected patients with undetectable viraemia

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    Klaudija Viskovic

    2014-11-01

    Full Text Available Introduction: Successfully treated HIV-infected patients may still have an increased risk for cardiovascular morbidity and mortality, which might be related not only to traditional risks, but also to inflammation and dyslipidemia induced by HIV and/or antiretroviral therapy [1, 2]. We examined the relationship of serum lipid levels with plasma biomarkers of inflammation using a composite inflammatory burden score (IBS from the following seven markers of inflammation: CD40L, tPA, MCP-1, IL-8, IL-6, hCRP and P-selectin. Materials and Methods: Subjects were selected among consecutive HIV-infected males ≥18 years of age with an undetectable viral load (75th percentile were considered elevated and an IBS was constructed as the presence of zero, one, two, or three or more elevated biomarkers. Correlations between the IBS and lipid parameters were examined using Spearman's Rho and by ordered logistic regression proportional odds model to estimate the odds of more elevated (>75th percentile biomarkers. Results: 181 male patients were included into the study, the median age was 46.7 (Q1–Q3, 39.9–55.0 years and the median current CD4 cell count was 553.0 (Q1–Q3, 389–729 per microliter. The patients were mainly treated with two nucleoside reverse transcriptase inhibitor (NRTI plus one non-NRTI (NNRTI (N=100, 60.8% or two NRTI plus lopinavir (N=50, 27.6%. There was a significant correlation between the IBS and serum cholesterol (Rho=0.23, 95% CI, 0.09–0.37, triglycerides (Rho=0.30, 95% CI, 0.16–0.42 and cholesterol/HDL-cholesterol ratio (Rho=0.25, 95% CI 0.11–0.38. In the multivariable model a one unit increase in cholesterol/HDL-cholesterol ratio was associated with a 1.72-fold (95% CI, 1.27–2.33 increased odds of having a greater IBS. One unit increase (mmol/L of cholesterol and triglycerides was associated with a 1.41-fold (95% CI, 1.13–1.76 and 1.37-fold (95% CI, 1.18–1.60 increased odds of having a greater IBS, respectively

  16. Association of the KLF14 rs4731702 SNP and Serum Lipid Levels in the Guangxi Mulao and Han Populations

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    Ping Huang

    2013-01-01

    Full Text Available The objective of the present study was to detect the association of the rs4731702 single nucleotide polymorphism (SNP and serum lipid levels in the Guangxi Mulao and Han populations. A total of 727 subjects of Mulao and 740 subjects of Han Chinese were included. Serum low-density lipoprotein cholesterol (LDL-C and apolipoprotein (Apo B levels were higher in Mulao than in Han (P<0.05. The T allele carriers had higher serum LDL-C and ApoAI levels in Mulao, whereas they had lower high-density lipoprotein cholesterol (HDL-C levels and ratio of ApoAI to ApoB in Han (P<0.05 than the T allele noncarriers. Subgroup analyses showed that the T allele carriers had higher HDL-C, LDL-C, and ApoAI levels in Mulao males and lower ApoAI levels and ratio of ApoAI to ApoB in Han males than the T allele noncarriers. The subjects with TT genotype in Han females also had higher total cholesterol, LDL-C, ApoAI, and ApoB levels than the subjects with CT or CC genotype. Serum lipid parameters were also correlated with several environmental factors in both ethnic groups. The differences in the association of KLF14 rs4731702 SNP and serum lipid levels between the two ethnic groups might partly result from different gene-environmental interactions.

  17. Coronary fat content evaluated by morphometry in patients with severe atherosclerosis has no relation with serum lipid levels

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    D.J. Braz Jr.

    2007-04-01

    Full Text Available The relationship between lipid serum levels and coronary atherosclerotic plaque fat content was studied in 51 necropsy patients. Serum lipids were measured by standard techniques, during life, in the absence of lipid-lowering drugs. Intima, intimal fat and media areas were measured using a computerized system in cryosections of the odd segments of the right, anterior descending and circumflex coronary arteries stained with Sudan-IV. Mean intimal and lipid areas were 5.74 ± 1.98 and 1.22 ± 0.55 mm² (22.12 ± 8.48% in 26 cases with high cholesterol (³200 mg/dL and 4.98 ± 1.94 and 1.16 ± 0.66 mm² (22.75 ± 9.06% in 25 cases with normal cholesterol ( 0.05. Patients with high levels of low-density lipoprotein (³130 mg/dL, N = 15 had a higher intima/media area ratio than those with normal levels of low-density lipoprotein (<130 mg/dL, N = 13, P < 0.01. No significant difference in the morphometrical variables was found in groups with high or low serum levels of triglycerides (³200 mg/dL, N = 13 vs <200 mg/dL, N = 36 or high-density lipoprotein (³35 mg/dL, N = 11 vs <35 mg/dL, N = 17. The association between the morphological measurements and serum levels of cholesterol, its fractions, and triglycerides was also tested and the correlation coefficients were low. Although high cholesterol is a risk factor, we show here that in patients with severe atherosclerosis blood cholesterol and triglyceride levels seem to have little influence on coronary lipid content, indicating that other factors may contribute to arterial lipid deposition and plaque formation.

  18. Nutrient intake, serum lipids and iron status of colligiate rugby players

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    Imamura Hiroyuki

    2013-02-01

    Full Text Available Abstract Background There are two main playing positions in rugby (backs and forwards, which demonstrate different exercise patterns, roles, and physical characteristics. The purpose of this study was: 1 to collect baseline data on nutrient intake in order to advise the athletes about nutrition practices that might enhance performance, and 2 to compare serum lipids, lipoproteins, apolipoproteins (apo, lecithin:cholesterol acyltransferase (LCAT activity, and iron status of forwards and backs. Methods The sporting group was divided into 18 forwards and 16 backs and were compared with 26 sedentary controls. Dietary information was obtained with a food frequency questionnaire. Results There were significant differences among the three groups. The forwards had the highest body weight, body mass index, percentage of body fat (calculated by sum of four skinfold thicknesses, as well as the highest lean body mass, followed by the backs and the control group. The mean carbohydrate intake was marginal and protein intake was lower than the respective recommended targets in all three groups. The mean intakes of calcium, magnesium, and vitamins A, B1, B2, and C were lower than the respective Japanese recommended dietary allowances or adequate dietary intakes for the rugby players. The forwards had significantly lower high-density lipoprotein cholesterol (HDL-C and HDL2-C than the backs and had significantly higher apo B and LCAT activity than the controls. The backs showed significantly higher HDL-C, HDL3-C, low-density lipoprotein cholesterol, and apo A-I, and LCAT activity than the controls. Four forwards (22%, five backs (31%, and three controls (12% had hemolysis. None of the rugby players had anemia or iron depletion. Conclusion The findings of our study indicate that as the athletes increased their carbohydrate and protein intake, their performance and lean body mass increased. Further, to increase mineral and vitamin intakes, we recommended athletes

  19. Ameliorating effect of black tea extract on cadmium chloride-induced alteration of serum lipid profile and liver histopathology in rats.

    Science.gov (United States)

    Mantur, Venkappa S; Somannavarib, Manjunath S; Yendigeri, Saeed; Das, Kusal K; Goudar, Shivaprasad S

    2014-01-01

    Cadmium is one among the most environmental pollutants that affects many organs like kidney, liver and testis. The present study was aimed to assess the simultaneous effects of black tea extracts (BTE) on cadmium chloride induced alterations in lipid profile and liver histology. Adult rats were divided into four groups (n=6/group), group I (normal saline), group II (CdCl2, 1.0 mg/kg, b.wt; i.p), group III (black tea extract, 2.5 gm tea leaf/dl of water that is 2.5% of aqueous BTE) and group IV (cadmium chloride + BTE). Cadmium chloride intoxicated rats showed significant increase in serum total cholesterol, triglycerides, and low density lipoprotein-cholesterol and there is a significant decrease in the serum high density lipoprotein-cholesterol. In the liver, cadmium chloride showed changes in normal architecture, swollen hepatocytes, kupffer cells hyperplasia, dilation and congestion of central vein. Oral administration of black tea extracts with cadmium chloride significantly improves lipid profile and liver architecture as compared to the cadmium chloride group. The results indicate that BTE is beneficial in preventing cadmium-induced lipid alterations and hepatocellular damage. PMID:25509961

  20. Comparison of serum lipid management between elderly and non-elderly patients with and without coronary heart disease (CHD).

    Science.gov (United States)

    Shimizu, Rumiko; Torii, Haruki; Yasuda, Daisuke; Hiraoka, Yoshinori; Furukawa, Yutaka; Yoshimoto, Akihiro; Iwakura, Toshio; Matsuoka, Naoki; Tomii, Keisuke; Kohara, Nobuo; Hashida, Tohru; Kume, Noriaki

    2016-12-01

    Serum lipid management in patients aged ≥ 75 has not been precisely explored. We, therefore, compared the serum lipid management between the two age groups with and without coronary heart disease (CHD). We, therefore, retrospectively reviewed medical charts of patients who were hospitalized in the departments of internal medicine during a period of 14 months. Serum lipid goal attainment was explored by applying the lipid goals for patients aged < 75 to those aged ≥ 75. In 1988 enrolled patients, 717 subjects (36.1%) were aged ≥ 75. Among them, 41.3% and 32.4% of the patients had CHD, 44.2% and 41.0% were primary prevention at high-risk, and 14.5% and 14.6% were primary prevention at moderate-risk in patients aged ≥ 75 and aged < 75, respectively. Serum LDL-C goal achievement rates in CHD were 66.9% and 65.0% in patients aged ≥ 75 and < 75, respectively (p = 0.334). In the primary prevention at high-risk, these rates were 73.5% and 63.3%, in patients aged ≥ 75 and < 75, respectively (p = 0.001). They were 77.9% and 58.1% in primary prevention at moderate-risk aged ≥ 75 and < 75, respectively (p < 0.001). In CHD, lipid-lowering medication subscription rates were significantly lower in patients aged ≥ 75 (60.1%) than those aged < 75 (73.8%, p < 0.001). In conclusion, in CHD, serum lipid goal attainment was comparable between the two age groups although the lipid-lowering drugs were less frequently prescribed in patients aged ≥ 75. Without CHD, it was significantly better in patients aged ≥ 75 than those aged < 75 although the lipid-lowering drug subscription rates were comparable between the two age groups. PMID:27413682

  1. Serum levels of FGF-21 are increased in coronary heart disease patients and are independently associated with adverse lipid profile.

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    Zhuofeng Lin

    Full Text Available BACKGROUND: Fibroblast growth factor 21 (FGF-21 is a metabolic regulator with multiple beneficial effects on glucose homeostasis and lipid metabolism in animal models. The relationship between plasma levels of FGF-21 and coronary heart disease (CHD in unknown. METHODOLOGY/PRINCIPAL FINDINGS: This study aimed to investigate the correlation of serum FGF-21 levels and lipid metabolism in the patients with coronary heart disease. We performed a logistic regression analysis of the relation between serum levels of FGF-21 and CHD patients with and without diabetes and hypertension. This study was conducted in the Departments of Endocrinology and Cardiovascular Diseases at two University Hospitals. Participants consisted of one hundred and thirty-five patients who have been diagnosed to have CHD and sixty-one control subjects. Serum FGF-21 level and levels of fasting blood glucose; triglyceride; apolipoprotein B100; HOMA-IR; insulin; total cholesterol; HDL-cholesterol; LDL-cholesterol; and C-reactive protein were measured. We found that median serum FGF-21 levels were significantly higher in CHD than that of control subjects (P<0.0001. Serum FGF-21 levels in CHD patients with diabetes, hypertension, or both were higher than that of patients without these comorbidities. Serum FGF-21 levels correlated positively with triglycerides, fasting blood glucose, apolipoprotein B100, insulin and HOMA-IR but negatively with HDL-C and apolipoprotein A1 after adjusting for BMI, diabetes and hypertension. Logistic regression analysis demonstrated that FGF-21 showed an independent association with triglyceride and apolipoprotein A1. CONCLUSIONS/SIGNIFICANCE: High levels of FGF-21 are associated with adverse lipid profiles in CHD patients. The paradoxical increase of serum FGF-21 in CHD patients may indicate a compensatory response or resistance to FGF-21.

  2. Serum lipid profile in patients with oral cancer and oral precancerous conditions

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    Rajul Mehta

    2014-01-01

    Full Text Available Background: The present study was undertaken to estimate and compare the levels of plasma total cholesterol (TC, low density lipoprotein (LDL, high density lipoprotein (HDL, very low density lipoprotein (VLDL and triglycerides in patients with oral precancerous lesions/conditions, oral cancer and normal subjects. Materials and Methods: The study comprised of 60 patients with oral precancerous lesions/conditions, 60 patients with oral cancer and a control group of 60 healthy individuals. The diagnosis of oral precancerous lesions/conditions and oral cancer was confirmed histopathologically. Under aseptic condition 5 ml venous blood of overnight fasting patient was withdrawn from each individual. Serum was separated by centrifugation and plasma levels of TC, LDL, HDL, VLDL and triglycerides were estimated. Descriptive statistical analysis has been carried out in the present study. Analysis of variance has been used to find the significance of study parameters between three or more groups of patients, Post-hoc test as Tukey has been used to find the pair wise significance. Significance is assessed at 5% level of significance. Results: Statistically significant decrease in levels of plasma TC, LDL, HDL, VLDL and triglycerides was observed in the precancerous and cancerous groups as compared to the control group. On comparison between precancerous and cancerous groups, significant decrease was observed in cancerous group. Conclusion: The change in lipid levels may have an early diagnostic or prognostic role in the oral premalignant lesions/conditions and oral cancer. The presence of decreased plasma lipid profile should increase the suspicion of these lesions to be investigated further.

  3. Miltefosine Affects Lipid Metabolism in Leishmania donovani Promastigotes▿

    OpenAIRE

    Rakotomanga, M.; Blanc, S.; Gaudin, K.; Chaminade, P.; Loiseau, P.M.

    2007-01-01

    Miltefosine (hexadecylphosphocholine [HePC]) is the first orally active antileishmanial drug. Transient HePC treatment of Leishmania donovani promastigotes at 10 μM significantly reduced the phosphatidylcholine content and enhanced the phosphatidylethanolamine (PE) content in parasite membranes, suggesting a partial inactivation of PE-N-methyltransferase. Phospholipase D activity did not seem to be affected by HePC. In addition, the enhancement of the lysophosphatidylcholine content could be ...

  4. The importance of serum lipids in exudative diabetic macular edema in type 2 diabetic patients.

    Science.gov (United States)

    Uçgun, Nil Irem; Yildirim, Zuhal; Kiliç, Nedret; Gürsel, Emin

    2007-04-01

    To evaluate the relationship between serum lipid levels and exudative diabetic maculopathy in patients with nonproliferative diabetic retinopathy, 27 patients with exudative diabetic macular edema were included in group A and 27 patients without exudative diabetic macular edema were included in group B. All 54 patients have nonproliferative diabetic retinopathy. Blood cholesterol, triglyceride, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, very low-density lipoprotein (VLDL) cholesterol, hemoglobin A1c (HbA1c), and hemoglobin levels were measured in patients in group A and group B. The mean concentration of cholesterol in group A (224.30 +/- 49.49 mg/dL), in group B (197.78 +/- 41.49 mg/dL); triglyceride in group A (199.11 +/- 90.51 mg/dL), in group B (160.78 +/- 65.30 mg/dL); HDL in group A (43.48 +/- 10.62 mmol/L), in group B (42.37 +/- 10.92 mmol/L); LDL in group A (150.59 +/- 43.96 mg/dL), in group B (124.37 +/- 40.28 mg/dL); VLDL in group A (40.52 +/- 16.54 mg/dL), in group B (37.89 +/- 23.70 mg/dL); HbA1c in group A (9.62 +/- 2.50), in group B (7.36 +/- 1.62 g/dL); and hemoglobin in group A (13.46 +/- 1.6 g/dL), in group B (13.90 +/- 1.77 g/dL). Serum cholesterol (P = 0.38), LDL (P = 0.026), and HbA1c (P = 0.000) levels were different between the two groups. Triglyceride, HDL, VLDL, and hemoglobin levels were not different between the two groups. We must consider regulation of high blood sugar and elevated total serum cholesterol or LDL levels in patients with macular edema and high hard exudates. PMID:17460181

  5. Effect of Ramadan Fasting on Serum Glucose and Lipid Profile Among Algerian Type 2 Diabetes Patients

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    Tiboura Ghania

    2015-12-01

    Full Text Available Background and aims: We designed this study to assess the effect of Ramadan fasting on serum lipid profile among type 2 diabetic patients. Material and Methods: The study was carried out in July 2014 (Ramadan 1421. The total duration of fasting was 17 hours a day. The investigation involved 80 patients. The mean age of the patients was 56± 8 years. The dietary survey lasting three days was recorded. The anthropometric and the biochemical parameters were measured in all subjects before (T1 and during (T2 the fasting month of Ramadan and results were compared using student t-test. Results: There was a significant decrease in high density lipoprotein cholesterol (HDL-c levels during T2 (0.35±0,08 g/L compared to T1 (0.38±0,11 g/L. Apolipoprotein A1 (Apo A1 decreased significantly during fasting compared to pre-fasting days while apo B increased during T2 (p˂0.05. The dietary fat consumption increased during Ramadan; especially for the saturated one (p<0.05. Conclusion: The present study suggests that fasting month of Ramadan could be beneficial for some patients with type 2 diabetes who are well controlled and balanced. However, some of them may be at risk of cardiovascular complications in which dyslipidemia can be the leading cause.

  6. Sesame and flaxseed oil: nutritional quality and effects on serum lipids and glucose in rats

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    Rita de Cássia Avellaneda Guimarães

    2013-03-01

    Full Text Available This study evaluated the nutritional value of sesame and flaxseed oils and their effects on the lipid and glucose profile of rats fed diets containing different fat combinations. Fatty acid composition, refractive index, and iodine and saponification values were analyzed to characterize the oils. In the biological assay, Wistar rats were fed different diets, whose fat composition consisted of varying combinations of flaxseed oil, sesame oil, and animal fat. The primary constituents of the sesame oil were oleic (28.6%, linoleic (28.4%, and lauric acid (14.6%; for the flaxseed oil they were alpha-linolenic (39.90%, oleic (17.97% and linoleic acid (12.25%. The iodine and saponification values of the oils were within the reference range. Rats fed flaxseed oil-based diets had the lowest serum cholesterol values, whereas rats fed diets with flaxseed oil + sesame oil + animal fat had the highest glucose levels. HDL levels decreased significantly with flaxseed oil. Sesame and flaxseed oils are sources of polyunsaturated fatty acids (PUFA, and the flaxseed oil-based diet had a hypocholesterolemic effect, whereas sesame oil showed oxidative stability since it contains high levels of monounsaturated and saturated fatty acids.

  7. The relationship between serum lipid levels, high blood pressure and obesity in children

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    Meltem Kurtuncu

    2014-03-01

    Full Text Available Objective: The purpose of this study was to determine serum lipid, cholesterol and obesity levels in healthy children, and then to explore the relationships between these factors. Methods: The sample group consisted of 103 students at Gelik Elementary School in Zonguldak, Turkey who was willing to participate in the study, had not been medically diagnosed with a chronic ailment, and were not taking regular medications. Results: When the students' mean BMI (Body Mass Index measurements were considered, it was found that mean BMI at 13 years of age (21.03 ± 0.23 was higher than at 14 years (20.05 ± 0.20 and that this difference was strongly significant (p=0.002. When the relationships between the students' height, weight, BMI, blood pressure, FBS (Fasting Blood Sugar, cholesterol, LDL (Low Density Lipoprotein, HDL (High Density Lipoprotein measurements were considered, it was seen that there was a positive significant relationship between height-weight (r=0.472; p<0.001, height-blood pressure (r=0.432; p<0.001, and height-FBS (r=0.332; p=0.001. Conclusions: The conclusion drawn was that monitoring blood pressure, cholesterol, LDL and HDL levels in childhood medical examinations is not only important in terms of identifying obesity, but also in identifying nutritional mistakes and deficiencies at an early stage.

  8. Association between Obesity, Serum Lipids, and Colorectal Polyps in Old Chinese People

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    Wen Yang

    2013-01-01

    Full Text Available Background. Colorectal cancer mostly arises from the polyps of colon. The aim of our study was to examine the association of body mass index (BMI and serum lipids with the colorectal polyps in old Chinese people. Methods. The risk of developing colorectal polyps was studied in 244 subjects (212 men and 32 women, 74.63 ± 11.63 years old who underwent colonoscopy for the first time from January 2008 to July 2012 at the Navy General Hospital, Beijing, China. According to the results of colonoscopy, all the subjects were divided into 112 normal control, 38 right colorectal polyps, 53 left colorectal polyps, and 41 both right and left colorectal polyps groups. The total plasma cholesterol, plasma triglyceride, plasma creatinine concentration, blood urinary nitrogen, and fasting glucose were determined using a multichannel analyzer. Results. There were significant differences among normal control, right colorectal polyps, left colorectal polyps, and both right and left polyps groups, which were the BMI, total cholesterol, triglycerides, creatinine, and urinary nitrogen. In binary logistic regression analysis, there were two risk factors associated with the occurrence of colorectal polyps, which included BMI and systolic blood pressure. Conclusions. Colorectal polyps were significantly associated with increased BMI, total cholesterol, and triglycerides levels.

  9. EFFECT OF DIETARY OLIVE OIL/CHOLESTEROL ON SERUM LIPOPROTEINS, LIPID PEROXIDATION, AND ATHEROSCLEROSIS IN RABBITS

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    R MAHDAVI

    2003-03-01

    Full Text Available Introduction: High plasma cholesterol levels, mainly LDL are a widely recognized major risk factor for Coronary Heart Disease (CHD. According to the epidemiologic studies findings, people from the Mediterranean countries, have lower CHD rats than other countries, in these countries usual diet is high in olive oil. The present study compares the effects of cholesterol enriched diet with or without adding olive oil on serum Lipoproteins, lipid per oxidation, and atherosclerosis development. Method: Twenty Dutch male rabbits were Categorized to four groups (one group as Control, and others as Experimental. They received one of standard, cholesterol - rich, olive oil rich and combined (cholesterol + olive oil diet for Twelve weeks. Fasting blood samples from heart were collected at the beginning, and the end of Experimental period. Means of total cholesterol, HDL-Ctriglycerides, MDA and antioxidant caperimental period, significant differences were showed in total cholesterol, HDL-C, triglyceride and MDA between groups. Results: The comparison of cholesterol rich diet with cholesterol + olive oil showed a higher mean of MDA in cholesterol rich group (P < 0.001. Biochemical factors and aortic lesion degree showed no significant difference between standard and olive oil group. Aortic lesions in cholesterol + olive oil showed nonsignificant lower degree than cholesterol group. Discussion: This findings showed preventive effect of olive oil against atherosclerosis which is independent of plasma lipoprotein effect, and suggested that probably olive oil acts on arteries directly.

  10. Lipid-rich bovine serum albumin improves the viability and hatching ability of porcine blastocysts produced in vitro

    OpenAIRE

    Suzuki, Chie; SAKAGUCHI, Yosuke; Hoshi, Hiroyoshi; Yoshioka, Koji

    2015-01-01

    The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts produced in vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium (PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching rates of blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were cultured with PBM alone, PBM containing LR-BSA, recombinant human serum albumin or fatty acid-free BSA, addit...

  11. Relationship Between Serum Leptin Concentration and Lipids & Body Mass Index in Patients With Chronic Congestive Heart Failure

    Institute of Scientific and Technical Information of China (English)

    Li Gang; Yan Qingbo; Wei Liangming

    2006-01-01

    Objectives To investigate the relationship of serum leptin concentration with the lipids and body mass index (BMI) in patients with chronic congestive heart failure(CHF). Methods The serum concentration of leptin in 39 patients with CHF (14 in cardiac function class Ⅱ , 21 in class Ⅲ , 4 in class Ⅳ , NYHA) and in 46 patients with cardiac function class Ⅰ (NYHA) were assessed by radioimmunoassay. Results The serum concentration of leptin were 9.018±4.519μg/l in CHF group (cardiac function class Ⅱ 11.492±5.649 μg/l, class Ⅲ 7.763±3.321 μg/l, class Ⅳ 6.100±2.657 μg/l);11.674±6.911 μg/l in class Ⅰ group. The serum concentrations of leptin were significantly lower in CHF group, as compared with class Ⅰ group (P<0.05). Moreover, the decrease of serum leptin concentration was significantly correlated with the decreased serum concentrations of total cholesterol, triglyceride, body mass index and left ventricular ejection fraction in CHF group, respectively (P<0.05). Conclusions The significance of the decrease in serum leptin in CHF patients needs further study.

  12. SERUM LEVEL OF LIPIDS, APOLIPOPROTEINS AND VITAMIN D IN CHILDREN AT HIGH RISK OF CORONARY ARTERY DISEASE

    Directory of Open Access Journals (Sweden)

    M BASHTAM

    2002-06-01

    Full Text Available Introduction. Coronary artery disease (CAD is one of the most prevalent disease in human population that has high prevalence and mortality in lsfahan (Iran. As positive family history and changes in serum lipids and lipoproteins are risk factors of these diseases, and also studies have showed the relationship between serum vitamin D and CAD, we studied serum level of lipids, lipoproteins and vitamin D in high risk children compared with control group, and the relation between serum vitamin D and other factors. Methods. This case-control study was done on 44 subjects (25 boys, 19 girls aged 2-18 years old with positive CAD family history as case group and also 44 persons with negative CAD family history as control group with the same age groups. The subjects were selected by convenience sampling method. Children who consumed antiepilepthic drugs as phenytoin or phenobarbital and those who had positive family histroy for renal stone were excluded for variable vitamin D levels due to drug interaction and genetic susceptibility, respectively. All subjects were invited to Isfahan Cardiovascular Research Center. Using a questionnaire, information on personal characteristics, CVD family history and ... were obtained. A fasting (12-14 hr blood sample was drawn from each one. Serum APOA1, APO B100 and vitamin Dwere measured by radioimmunoassay and serum lipids by ELAN 2000 autoanalyzer. Statistical analysis was done by SPSS. The level of serum APOA1, APO B100 and vitamin D were compared between two groups by independent t test and the relation of the mentioned apolipoproteins with vitamin D was studied using multiple linear regression. Results. Serum vitamin D was significantly lower in case group (P < 0.045. Among studied factors, only triglyceride was significantly higher in control group (P < 0.0001 and also no significant relaitonship was observed between serum APO A1, APO B100 and vitamin D. Sex comparision in case group showed those mean levels

  13. Effects of Crude Extract of Ageratum Conyzoides, Moringa Oleifera and Zanthoxylum Zanthoxyloides on Serum Lipid Profile in Albino Rats

    OpenAIRE

    OYEWOLE O.I.; ADEBAYO A.G.; OGUNSAKIN S.M

    2012-01-01

    Ageratum conyzoides, Moringa oleifera and Zanthoxylum zanthoxyloides are among medicinal plants commonly used in African folk medicine as remedies for the treatments of high blood pressure and heart related diseases. This study was carried out to compare the effects of Ageratum conyzoides, Moringa oleifera and Zanthoxylum zanthoxyloides extracts on serum lipid profile in albino rats. Thirty five albino rats were grouped into seven each containing five rats. Control rats (Group 1) received dis...

  14. Effect of a MAST Exercise Program on Anthropometric Parameters, Physical Fitness, and Serum Lipid Levels in Obese Postmenopausal Women

    OpenAIRE

    Trabka Bartosz; Zubrzycki Igor Z.; Ossowski Zbigniew; Bojke Olgierd; Clarke Anna; Wiacek Magdalena; Latosik Ewelina

    2014-01-01

    The purpose of this study was to examine an influence of a mixed aerobic and strength training program (MAST) on anthropometry, serum lipid levels, physical performance, and functional fitness in obese postmenopausal women. The MAST sessions were held three times per week, and the exercise program lasted for 10 weeks. The exercise group demonstrated a statistically significant improvement in maximal oxygen uptake, a waist/hip ratio, and strength of the upper and lower body. An increase in LDL...

  15. The effect of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclosporine-treated rats

    OpenAIRE

    Khadir, Fatemeh; Pouramir, Mahdi; Joorsaraee, Seyyed Gholamali; Feizi, Farideh; Sorkhi, Hadi; Yousefi, Fatemeh

    2015-01-01

    Background: Cyclosporine A (CsA) is a potent immunosuppressant drug with therapeutic and toxic actions. The use of CsA is limited by its toxicity. Several researchers had proposed that oxidative stress could play an important role in CsA-induced toxicity. Arbutin has recently been shown to possess antioxidative and free radical scavenging abilities. The present study was designed to investigate the in vivo effects of arbutin on lipid peroxidation and antioxidant capacity in the serum of cyclo...

  16. Serum Calcium Increase Correlates With Worsening of Lipid Profile: An Observational Study on a Large Cohort From South Italy.

    Science.gov (United States)

    Gallo, Luigia; Faniello, Maria C; Canino, Giovanni; Tripolino, Cesare; Gnasso, Agostino; Cuda, Giovanni; Costanzo, Francesco S; Irace, Concetta

    2016-02-01

    Despite the well-documented role of calcium in cell metabolism, its role in the development of cardiovascular disease is still under heavy debate. Several studies suggest that calcium supplementation might be associated with an increased risk of coronary heart disease, whereas others underline a significant effect on lowering high blood pressure and hyperlipidemia. The purpose of this study was to investigate, in a large nonselected cohort from South Italy, if serum calcium levels correlate with lipid values and can therefore be linked to higher individual cardiovascular risk.Eight-thousand-six-hundred-ten outpatients addressed to the Laboratory of Clinical Biochemistry, University of Magna Græcia, Catanzaro, Italy from January 2012 to December 2013 for routine blood tests, were enrolled in the study. Total HDL-, LDL- and non-HDL colesterol, triglycerides, and calcium were determined with standard methods.We observed a significant association between total cholesterol, LDL-cholesterol, HDL-cholesterol, non-HDL cholesterol, triglycerides, and serum calcium in men and postmenopause women. Interestingly, in premenopause women, we only found a direct correlation between serum calcium, total cholesterol, and HDL-cholesterol. Calcium significantly increased while increasing total cholesterol and triglycerides in men and postmenopause women.Our results confirm that progressive increase of serum calcium level correlates with worsening of lipid profile in our study population. Therefore, we suggest that a greater caution should be used in calcium supplement prescription particularly in men and women undergoing menopause, in which an increase of serum lipids is already known to be associated with a higher cardiovascular risk. PMID:26937904

  17. Gene Transfection in High Serum Levels: Case Studies with New Cholesterol Based Cationic Gemini Lipids

    OpenAIRE

    Misra, Santosh K; Biswas, Joydeep; Kondaiah, Paturu; Bhattacharya, Santanu

    2013-01-01

    Background: Six new cationic gemini lipids based on cholesterol possessing different positional combinations of hydroxyethyl (-CH2CH2OH) and oligo-oxyethylene -(CH2CH2O)(n)- moieties were synthesized. For comparison the corresponding monomeric lipid was also prepared. Each new cationic lipid was found to form stable, clear suspensions in aqueous media. Methodology/Principal Findings: To understand the nature of the individual lipid aggregates, we have studied the aggregation properties using ...

  18. Serum lipid profile in non-polycystic ovary syndrome and polycystic ovary syndrome women: a comparative and correlational study

    OpenAIRE

    Nimish R. Halasawadekar; Jaiprakash B Ramanand; Ramanand, Sunita J.; Girish T. Raparti; Praveenkumar T. Patil; Ruchi D. Shah; Arvind V. Kumbhar

    2016-01-01

    Background: Polycystic ovary syndrome (PCOS), in addition to impaired ovulation, also affects metabolic pathways. Dyslipidemia, occurring in PCOS women leads to cardiovascular diseases in them. The purpose of the present study was to compare lipid profile and its correlation with biochemical and hormonal parameters in PCOS and non PCOS women, to analyse the correlation of lipid profile with hirsutism and body mass index (BMI) in PCOS women. Methods: The present study includes 68 women divi...

  19. Relationship and significance of serum leptin with blood insulin and lipid in 6-13 years old obese children

    International Nuclear Information System (INIS)

    To explore relationship and significance of Serum Leptin with BMI, Insulin, triglyceride (TG) and total cholesterol (TC) in obese children aged 6-13 years. Serum Leptin of school-age children 118 (64 male, 54 female; normal non-obese 56 and obese 62) were deter- mined and compared with BMI, Insulin, TG and TC. The results showed that: (1) Each index of obese children was remarkably higher than that of non-obese children (P0.05). (3) Leptin was poritinely corelation with BMI, insulin, TG and TC(P=0.001). Leptin level in serum may varied according to sex, BMI or blood lipid level. It is of great significance in prevention and treatment of obesity to use drug which may improve Leptin receptor effect. (authors)

  20. Sex Hormones Affect Aging Process by Influencing Lipid Profiles,Cellular Immunological Function and Lipid Peroxides and Oxidation System

    Institute of Scientific and Technical Information of China (English)

    吴赛珠; 谭家余; 周忠江; 周可祥; 容志毅

    2003-01-01

    Objectives To investigate the correlation between sex hormones(SH) and aging. Methods Through epidemiological investigation in our country, the levels of SH were measured by radioimmunoassy; lipid profile, glucose and apolipoprotein by automatic biochemic analytical instrument; T cell subsets by flow cytometer; and MDA, SOD were evaluated by the thiobarbituric acid (TBA) test and the nitrite method modified by Oyanagui respectively using spectrophotometry. Results In men, the serum levels of follicle stimulating hormone (FSH)、 luteinizing hormone(LH) increased significantly with aging, but serum prolactin(PRL) and progesterone(P) levels remained unchanged in all life; Both testosterone (T)and free testosterone (FT) all decreased greatly with aging, but 173 - estradiol( 17β - E2) was reverse; E2 was negatively correlated with T and E2/T increased with aging. The level of serum total cholesterol (TC) increased with aging, but triglycerides ( TG ) remain unchanged; compared with young group, high -density lipoprotein cholesterol (HDL - C); HDL - C/TC of other groups decreased significantly, but low - density lipoprotein cholesterol ( LDL - C ) changed inversely;HDL- C/LDL- C reduced slightly with aging and showed no difference between groups. Apolipoprotein A1 (apoA1) and apoB all enhanced greatly with aging;meanwhile the ratio of apoAl/apoB decreased. The concentration of serum glucose (GLU) was unchanged in all life. To compare with those in the young group,CD3 + , CD4 + in other groups reduced greatly, CD4 + remained unchanged. Meanwhile, CD8 + increased significantly with aging. Compared with the young group, serum malondialdehyde(MDA) value of the old ones increased obviously, but the activity of superoxide dismutase(SOD) was reverse. By partial correlation analysis (controlling BMI, FSH, LH and PRL), TC、LDL- C、apoA1 、apoB、CD8 + 、MDA of men all presented a positive correlation with E2/T respectively,their correlation coefficients (γ) were 0.262、0

  1. Providing lipid-based nutrient supplements does not affect developmental milestones among Malawian children

    Science.gov (United States)

    Our objective was to assess whether using lipid-based nutrient supplements (LNS) to complement the diets of infants and young children affected when they achieved selected developmental milestones. In rural Malawi, 840 6-month-old healthy infants were enrolled to a randomised trial. Control particip...

  2. Analysis of serum lipid profiles, metal ions and thyroid hormones levels abnormalities in - thalassaemic children of Bangladesh

    International Nuclear Information System (INIS)

    Objective: To assess the serum lipid profile of cardiovascular disease free male and female children with - thalassaemia. Levels of zinc, copper and magnesium in the serum were also determined along with the Thyroid profile. Methods: From January to December 2007, we enrolled 121 consecutive patients with -thalassaemia that visited The Thalassaemia Center at Dhaka Shishu (Children) Hospital, Bangladesh every month for routine examinations. Fasting blood lipid levels were measured in all participants. Zinc, Copper and Magnesium levels in serums were determined. Thyroid function was also assessed by evaluating T3, T4 and TSH levels. Results: Of the 121 patients, 65 were males (10.14 +- 3.91 years) and 56 were females (9.08 +- 4.32 years). Data analysis revealed that 2.0% males and 4.35% females had high total serum cholesterol, and 28.57% males and 21.74% females had high triglyceride levels. In addition, mean HDL-cholesterol levels were 21.14 +- 5.82 mg/dl in males and 21.17 +- 6.02 mg/dl in females; total-cholesterol to HDL-cholesterol ratios were 5.47 +- 1.66 and 5.96 +- 2.81 in males and females respectively. About 60% patients showed low serum level of Zn and Cu. Hypothyroidism was detected in 30% patients and 23% patients had abnormal experimental values of all the study parameters. Conclusions: The majority of the patients had blood lipid levels (by the exception of HDL-cholesterol) within the normal range, and consequently the prevalence of lipid abnormalities was much lower as compared to the general population of the same age. Interestingly, the total-cholesterol to HDL-cholesterol ratio was high in our patients, and may underline the importance of this index for the prognosis of future cardiac events in these patients. The serum Zn and Cu levels were low in most of the patients which may cause some metabolic abnormalities in future. Most of the patients also showed hypothyroidism indicating the presence of endocrine complications. (author)

  3. Serum TSH Level in Healthy Koreans and the Association of TSH with Serum Lipid Concentration and Metabolic Syndrome

    OpenAIRE

    Lee, Yeo Kyung; Kim, Jo Eun; Oh, Hyun Ju; Park, Kyung Sun; Kim, Soo Kyung; Park, Seok Won; Kim, Moon Jong; Cho, Yong Wook

    2011-01-01

    Background/Aims The proper treatment of subclinical hypothyroidism and the normal range of serum thyroid stimulating hormone (TSH) concentration are intensely debated. However, few reports have investigated TSH concentrations in Asian ethnic groups. Therefore, the present study was designed to define the TSH reference range in a Korean population and to investigate the metabolic significance of TSH concentration. Methods We enrolled patients who underwent medical examination at the CHA Bundan...

  4. Determination of RBC membrane and serum lipid composition in trinidadian type II diabetics with and without nephropathy

    Directory of Open Access Journals (Sweden)

    B Shivananda Nayak

    2008-09-01

    Full Text Available B Shivananda Nayak1, Vishi Y Beharry1, Shivani Armoogam1, Melinda Nancoo1, Kevin Ramadhin1, Kiron Ramesar1, Ciara Ramnarine1, Anandi Singh1, Anisha Singh1, Kingsley Uche Nwachi1, Surujpaul Teelucksing2, Ramesh Mathura3, Lesley Roberts41Department of Preclinical Sciences, Biochemistry Unit, 2Department of Clinical Science, The University of the West Indies, EWMSC, Trinidad and Tobago; 3Department of Diagnostic Laboratory Services, NCRHA, EWMSC, Trinidad and Tobago; 4Department of Medicine, Nephrology Unit, NCRHA, EWMSC, Trinidad and TobagoAim: The rheological properties of erythrocytes are impaired in diabetes mellitus, especially because of changes in their membrane lipid composition.The aim of this study was to determine and examine the relationship between red blood cell (RBC membrane and serum lipid composition in type II diabetes subjects with and without nephropathy.Methods: Trinidadian subjects aged 18–65 years were recruited for the study regardless of gender and ethnicity. Fasting blood samples were collected from 60 subjects of whom 20 were healthy individuals, 20 had type II diabetes without complications, and 20 were type II diabetics with nephropathy. Weight, height, waist/hip ratio, and blood pressure were recorded. All the blood samples were analysed to determine the serum lipid concentration, membrane lipid composition and plasma glucose concentration.Results: The body mass index and the systolic blood pressure of the diabetics (28.17 ± 4.98 kg/m2, 153.21 ± 22.10 mmHg and those with nephropathy (25.87 ± 4.68, 158.60 ± 22.49 mmHg were higher when compared with controls (24.67 ± 5.18, 119.15 ± 13.03 mmHg. The diabetic (175.89 ± 102.73 μg/mgprotein and diabetic nephropathy (358.80 ± 262.66 subjects showed significantly higher levels of RBC membrane cholesterol compared with controls (132.27 ± 66.47. The membrane phospholipids, protein and Na+/K+ATPase concentrations were altered in diabetics and diabetic nephropathy

  5. Determination of RBC membrane and serum lipid composition in trinidadian type II diabetics with and without nephropathy

    Directory of Open Access Journals (Sweden)

    B Shivananda Nayak

    2008-08-01

    Full Text Available B Shivananda Nayak1, Vishi Y Beharry1, Shivani Armoogam1, Melinda Nancoo1, Kevin Ramadhin1, Kiron Ramesar1, Ciara Ramnarine1, Anandi Singh1, Anisha Singh1, Kingsley Uche Nwachi1, Surujpaul Teelucksing2, Ramesh Mathura3, Lesley Roberts41Department of Preclinical Sciences, Biochemistry Unit, 2Department of Clinical Science, The University of the West Indies, EWMSC, Trinidad and Tobago; 3Department of Diagnostic Laboratory Services, NCRHA, EWMSC, Trinidad and Tobago; 4Department of Medicine, Nephrology Unit, NCRHA, EWMSC, Trinidad and TobagoAim: The rheological properties of erythrocytes are impaired in diabetes mellitus, especially because of changes in their membrane lipid composition.The aim of this study was to determine and examine the relationship between red blood cell (RBC membrane and serum lipid composition in type II diabetes subjects with and without nephropathy.Methods: Trinidadian subjects aged 18–65 years were recruited for the study regardless of gender and ethnicity. Fasting blood samples were collected from 60 subjects of whom 20 were healthy individuals, 20 had type II diabetes without complications, and 20 were type II diabetics with nephropathy. Weight, height, waist/hip ratio, and blood pressure were recorded. All the blood samples were analysed to determine the serum lipid concentration, membrane lipid composition and plasma glucose concentration.Results: The body mass index and the systolic blood pressure of the diabetics (28.17 ± 4.98 kg/m2, 153.21 ± 22.10 mmHg and those with nephropathy (25.87 ± 4.68, 158.60 ± 22.49 mmHg were higher when compared with controls (24.67 ± 5.18, 119.15 ± 13.03 mmHg. The diabetic (175.89 ± 102.73 μg/mgprotein and diabetic nephropathy (358.80 ± 262.66 subjects showed significantly higher levels of RBC membrane cholesterol compared with controls (132.27 ± 66.47. The membrane phospholipids, protein and Na+/K+ATPase concentrations were altered in diabetics and diabetic nephropathy

  6. Effect of substratum, serum and linoleic acid on the lipid synthesis of isolated alveolar type II cells

    International Nuclear Information System (INIS)

    The authors examined the effect of cellular substratum (plastic or amnionic basement membrane (ABM)) and serum additive (fetal bovine (FBS), pork, horse, rat or human) on phospholipid synthesis in alveolar type II cells. The cells were isolated from adult rats, cultured for 48 hours under the various substratum and serum conditions, and then incubated for an additional 2 hours with [1-14C] acetate. ABM consistently caused a significant increase in the percent of radiolabel incorporated into phosphatidylcholine (PC) and/or phosphatidylglycerol (PG). Serum also had a significant effect with the highest values of PC and saturated PC being obtained with rat serum and the highest PG values with horse serum. The fatty acid composition of the sera used varied according to species with the largest variations in percent linoleic acid. Supplementing media with linoleic acid resulted in a marked increase in saturated PC values and a fall in PG values. Therefore, they conclude that: 1) ABM improves differentiated function, 2) FBS supplementation may not be optimal, and 3) the different effects of linoleic acid supplementation on PC, saturated PC, and PG values suggests an independent regulation of synthesis for these lipid species in vitro

  7. Studies on the correlation of TF, SF, Hb in serum affected virus hepatitis with RIA

    International Nuclear Information System (INIS)

    The TF, SF and Hb in serum of 119 cases affected virus hepatitis are determined by RIA. The result indicates that the TF in serum for the different type of virus hepatitis decreases comparing with the normal and the TF has a tendency to decrease remarkably as the condition of patients get worse. It also shows that the TF has the negative association with the SF, whereas the TF has the positive with Hb. However, they have no significant association (P>0.05) although the correlated coefficients get larger as the condition of the patients get worse

  8. The Effects of Short-Term Intensive Exercise on Levels of Liver Enzymes and Serum Lipids in Kick Boxing Athletes

    Directory of Open Access Journals (Sweden)

    Ömer Kaynar

    2016-03-01

    Full Text Available Objective: In this study, it was aimed to evaluate the ef­fects of short-term intensive exercise on liver enzymes and serum lipid levels with kick boxing athletes. Methods: 23 voluntary athletes who were between the ages of 15-46 and who engaged in kick–boxing have tak­en place this study. Athletes were made to do 45 minutes of warming-up, breathing, and stretching and 50 minutes of technical and tactical practices and then they were made to do a training match, which is equal to a 2 min­utes 3 circuits (1 minute rest kick-box match. In venous blood samples which were taken from athletes before and after training, aspartate aminotransferase (AST, alanine aminotransferase (ALT, alkaline phosphatase (ALP and gamma glutamine transpeptidase (GGT, enzyme activity and total cholesterol, high-density lipoprotein-cholesterol (HDL-C, low density lipoprotein-cholesterol (LDL-C and triglycerides serum levels were analyzed via spectropho­tometric method in Beckman Coulter AU 5800 auto ana­lyzer. Body composition measurements of athletes were made with Tanita TBF 300 brand device, which works with bio-impedance analysis (BIA system. Results: As a result of our study, statistically increases in serum ALT, AST, ALP and GGT enzyme activities and in serum total cholesterol, HDL-C and LDL-C levels were detected following short-term intensive exercise, but no significant difference was observed in TG levels after in­tensive exercise. Conclusion: The blows to the abdomen during kickbox­ing sports competitions result in increased liver enzymes and increased serum lipids may occur to meet energy de­mand of the body during exercise.

  9. Study of the Effect of Garlic on Serum Lipids and Blood Glucose Levels in Type 2 Diabetic Patients

    Directory of Open Access Journals (Sweden)

    M Afkhami - Ardekani

    2005-04-01

    Full Text Available Introduction: Hyperlipidemia and diabetes are common risk factors for ischemic heart disease, which is the main cause of mortality in diabetic patients. Strict control of blood glucose and other risk factors in diabetics has led to prevention of complications. Garlic has received particular attention for control of blood glucose and decrease in blood lipid levels. At present, several studies have been carried out in order to prove advantages of garlic. Methods: In this study, effects of garsin (a derivative of garlic present in our country on serum lipids and blood glucose levels in diabetes mellitus type 2 patients was observed. Forty-five type 2 diabetics who had hyperlipidemia were selected. These patients were kept on treatment with 3 tablets of Garsin / day for 4 weeks. Serum lipids and blood glucose levels were measured prior to and at the end of treatment. Results: Relationship between sex and response to treatment in this study was meaningful, such that Gsarsin led to decrease in LDL-C and increase in HDL in females. Conclusion: Therefore, Garsin can be used as an adjunct to treatment in diabetes type 2 patients with hyperlipidemia.

  10. Serum Adiponectin and Leptin Concentrations in Relation to Body Fat Distribution, Hematological Indices and Lipid Profile in Humans

    Directory of Open Access Journals (Sweden)

    Anna Lubkowska

    2015-09-01

    Full Text Available The purpose of the study was to evaluate the relationship between serum adiponectin and leptin concentrations and body composition, hematological indices and lipid profile parameters in adults. The study involved 95 volunteers (BMI from 23.3 to 53 kg/m2. Anthropometric parameters were measured: body weight and height, waist and hip circumference, waist-to-hip ratio, body fat mass (BMF, subcutaneous and visceral fat mass (SFM, VFM, lean body mass (LBM, skeletal muscle mass (SMM. In serum we determined adiponectin and leptin concentrations, extracellular hemoglobin, total bilirubin, as well as lipid metabolism (TCh, HDL-Ch, LDL-Ch, TG. Mean adipokine levels were significantly higher in women (p ≤ 0.01, adiponectin significantly negatively correlated with body height and weight, systolic blood pressure and absolute LBM and SMM values. The same relation was observed for erythroid system indicators and lipid indicators. A positive correlation was exceptionally found between adiponectin and HDL-Ch. LEP negatively correlated with some percentage rates (%LBM, %SMM. Only in women, we observed a positive correlation between LEP and body weight, BMI and WHR. Studies on ADPN and the ADPN/LEP ratio as a valuable complementary diagnostic element in the prediction and prevention of cardiovascular diseases need to be continued.

  11. A comparative study on serum lipoprotein (a and lipid profile between rheumatoid arthritis patients and normal subjects

    Directory of Open Access Journals (Sweden)

    K P Shiva Govindan

    2015-01-01

    Full Text Available Background: Rheumatoid arthritis is a chronic and systemic inflammatory disorder, in which Lipoprotein (a [Lp (a] increases plaque formation and thus promotes atherosclerosis. Coronary artery disease is one of the co-morbidity in rheumatoid arthritis patients. Aim: The aim of this study is to evaluate Lp (a as a cardiovascular risk factor in patients with rheumatoid arthritis. This was a comparative study in which Lp (a and lipid profile were compared in rheumatoid arthritis patients and controls. Materials and Methods: The study included 30 sero-positive rheumatoid arthritis patients and 30 normal healthy subjects with an age and sex matched group of 25-80 years. Statistical analysis was performed using SPSS version 17. Results: Serum Lp (a concentration was significantly increased (P < 0.001 in rheumatoid arthritis patients compared with controls. Serum high-density lipoprotein-cholesterol was significantly lowered (P < 0.05 in patients as compared to controls. There was no significant difference in serum total cholesterol, triglycerides, and very low density lipoprotein-cholesterol between patients and controls. Conclusion: The findings indicate that the patients with rheumatoid arthritis are at high risk of developing cardiovascular disease in future due to the increased level of Lp (a. In addition to conventional lipid profile, estimation of Lp (a can prove to be a valuable tool in risk assessment of population in general and management of disease in particular.

  12. A long-term follow-up study of serum lipid levels and coronary heart disease in the elderly

    Institute of Scientific and Technical Information of China (English)

    李健斋; 陈曼丽; 王抒; 董军; 曾平; 侯鲁维

    2004-01-01

    Background It is still controversial whether or not the correlation between lipid abnormality and coronary heart disease (CHD) becomes weaker in the elderly, and whether patients above 80 years old still benefit from lipid management for the secondary prevention of CHD. The purpose of this study is to assess the correlation between hyperlipidemia and the risk of CHD events in the elderly, and to determine if it is appropriate to use lipid-lowering drugs in those aged above 80, as prescribed by the recommended guidelines for lipid management.Methods One thousand two hundred and eleven retirees, mainly males (92%), aged 70±9 years, were enrolled in this study. Lifestyle habits and medical history were recorded via questionnaires. During the period 1986 -2000, all subjects participated in an annual physical examination with a blood chemistry survey. The mean follow-up period was 11.2 years. Subjects with incidental illnesses, especially cardiovascular diseases, were diagnosed or treated promptly. Serum lipid parameters, including total cholesterol (TC), Iow and high-density lipoprotein cholesterol (LDL-C and HDL-C) and triglyceride (TG) levels were analyzed according to standardization of lipid and lipoprotein measurements. The association between lipid levels and the prevalence of acute myocardial infarction (AMI) or coronary death was analyzed statistically. Results Lipid abnormalities occurred in 2/3 of the 1211 subjects. The most common lipid disorder was high TC and high LDL-C, which was much more prevalent than high TG. Among the subjects, 51.6% had TC levels above 5.2 mmol/L. Mean TC and LDL-C reached peak levels in the 65-74 age group without significant decrease until ages over 90. The cumulative total number of deaths due to various causes was 397 in the 15-year follow-up period, with the mortality rate in the high lipid group slightly lower than that in the normal lipid group (30.6% vs 35. 3% ), although the difference was not significant (P=0

  13. Effect of Garlic (Allium sativum) on Heavy Metal (Nickel II and ChromiumVI) Induced Alteration of Serum Lipid Profile in Male Albino Rats

    OpenAIRE

    Kusal K. Das; Dhundasi, Salim A; Swastika N. Das; Amrita Das Gupta

    2008-01-01

    We have studied the effect of simultaneous oral treatment of aqueous garlic extract (Allium sativum) on heavy metal (nickel II and chromium VI) induced changes in serum lipid profile. Nickel sulfate and potassium dichromate treated rats showed a significant increase in serum low density lipoprotein-cholesterol (LDL-C), very low density lipoprotein-cholesterol (VLDL-C) and triglyceride (TG) level as well as decrease in serum high density lipoprotein-cholesterol (HDL-C) level. Simultaneous garl...

  14. A lignan complex isolated from flaxseed does not affect plasma lipid concentrations or antioxidant capacity in healthy postmenopausal women

    DEFF Research Database (Denmark)

    Hallund, Jesper; Ravn-Haren, Gitte; Bügel, S.;

    2006-01-01

    A lignan complex rich in the plant lignan secoisolariciresinol diglucoside (SDG) was isolated from flaxseed. SDG is metabolized by the colonic microflora to the mammalian lignans enterodiol (END) and enterolactone (ENL), and was hypothesized to reduce plasma lipid concentrations and improve...... antioxidant capacity, The aim of this study was to investigate the effects of a lignan complex, providing 500 mg/d of SDG, on serum concentration and urinary excretion of ENL, plasma lipids, serum lipoprotein oxidation resistance, and markers of antioxidant capacity. Healthy postmenopausal women (n=22...

  15. The relationship between serum lipid levels and the risk of oral cancer

    Directory of Open Access Journals (Sweden)

    Jyoti G Chawda

    2011-01-01

    Full Text Available Background: Lipids are the major cell membrane components essential for various biological functions including cell growth and division for the maintenance of cell integrity of normal and malignant tissues. The changes in lipid profile have long been associated with cancer. Hypocholesterolemia has been observed in patients with cancers of various organs. Therefore, a causative relationship might exist between plasma lipid levels and oral cancer patients. Aim: The objective of this study was to investigate the alterations and clinical significance of plasma lipid profiles in untreated head and neck cancer patients. Materials and Methods: A total of 30 subjects (25 oral cancer patients and 5 controls were included. Fasting blood lipid profile including cholesterol (C, triglycerides (TG, high density lipoprotein (HDL, low density lipoprotein (LDL and very low density lipoprotein (VLDL were evaluated using spectrophotometric kits, with CHOD PAP technique. The values were then statistically analyzed using analysis of variance (ANOVA and post hoc methods. Results: The levels of total lipids, cholesterol and HDL were significantly lower in oral cancer patients as compared to controls, but LDL and VLDL values were not significant. Conclusion: An inverse relationship was found between the lipid levels and the occurrence of oral cancer. Hence, the lower plasma lipid status may be a useful indicator to detect the initial changes seen in neoplastic process.

  16. Fetal and life course origins of serum lipids in mid-adulthood: results from a prospective cohort study

    Directory of Open Access Journals (Sweden)

    Gustafsson Per E

    2010-08-01

    Full Text Available Abstract Background During the past two decades, the hypothesis of fetal origins of adult disease has received considerable attention. However, critique has also been raised regarding the failure to take the explanatory role of accumulation of other exposures into consideration, despite the wealth of evidence that social circumstances during the life course impact on health in adulthood. The aim of the present prospective cohort study was to examine the contributions of birth weight and life course exposures (cumulative socioeconomic disadvantage and adversity to dyslipidemia and serum lipids in mid-adulthood. Methods A cohort (effective n = 824, 77% was prospectively examined with respect to self-reported socioeconomic status as well as stressors (e.g., financial strain, low decision latitude, separation, death or illness of a close one, unemployment at the ages of 16, 21, 30 and 43 years; summarized in cumulative socioeconomic disadvantage and cumulative adversity. Information on birth weight was collected from birth records. Participants were assessed for serum lipids (total cholesterol, low- and high-density lipoprotein cholesterol and triglycerides, apolipoproteins (A1 and B and height and weight (for the calculation of body mass index, BMI at age 43. Current health behavior (alcohol consumption, smoking and snuff use was reported at age 43. Results Cumulative life course exposures were related to several outcomes; mainly explained by cumulative socioeconomic disadvantage in the total sample (independently of current health behaviors but attenuated by current BMI and also by cumulative adversity in women (partly explained by current health behavior but not by BMI. Birth weight was related only to triglycerides in women, independently of life course exposures, health behaviors and BMI. No significant association of either exposure was observed in men. Conclusions Social circumstances during the life course seem to be of greater importance

  17. The Effects of Short-Term Intensive Exercise on Levels of Liver Enzymes and Serum Lipids in Kick Boxing Athletes

    OpenAIRE

    Ömer Kaynar; Nurinnisa Öztürk; Fatih Kıyıcı; Nurcan Kılıç Baygutalp; Ebubekir Bakan

    2016-01-01

    Objective: In this study, it was aimed to evaluate the ef­fects of short-term intensive exercise on liver enzymes and serum lipid levels with kick boxing athletes. Methods: 23 voluntary athletes who were between the ages of 15-46 and who engaged in kick–boxing have tak­en place this study. Athletes were made to do 45 minutes of warming-up, breathing, and stretching and 50 minutes of technical and tactical practices and then they were made to do a training match, which is equal to a 2 min­u...

  18. Influence of contraceptive pill and menstrual cycle on serum lipids and high-density lipoprotein cholesterol concentrations.

    OpenAIRE

    Demacker, P N; Schade, R W; Stalenhoef, A F; Stuyt, P M; Van 't Laar, A

    1982-01-01

    The fluctuations of serum lipid and lipoprotein concentrations within one cycle were studied both in women using and not using oral contraceptives. High-density lipoprotein cholesterol decreased significantly from 1.47 mmol/l (57 mg/100 ml) to 1.30 mmol/l (50 mg/100 ml) during one contraceptive cycle in eight women and rose again to the initial value during the pill-free days. The mean concentration of total cholesterol also fell significantly as a result of the decrease of high-density lipop...

  19. Dietary lipid and gross energy affect protein utilization in the rare minnow Gobiocypris rarus

    Science.gov (United States)

    Wu, Benli; Xiong, Xiaoqin; Xie, Shouqi; Wang, Jianwei

    2015-10-01

    An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the effects of protein to energy ratio on growth, for the rare minnow (Gobiocypris rarus), which are critical to nutrition standardization for model fish. Twenty-four diets were formulated to contain three gross energy (10, 12.5, 15 kJ/g), four protein (20%, 25%, 30%, 35%), and two lipid levels (3%, 6%). The results showed that optimal dietary E/P was 41.7-50 kJ/g for maximum growth in juvenile rare minnows at 6% dietary crude lipid. At 3% dietary lipid, specific growth rate (SGR) increased markedly when E/P decreased from 62.5 kJ/g to 35.7 kJ/g and gross energy was 12.5 kJ/g, and from 75 kJ/g to 42.9 kJ/g when gross energy was 15.0 kJ/g. The optimal gross energy was estimated at 12.5 kJ/g and excess energy decreased food intake and growth. Dietary lipid exhibited an apparent protein-sparing effect. Optimal protein decreased from 35% to 25%-30% with an increase in dietary lipid from 3% to 6% without adversely effecting growth. Dietary lipid level affects the optimal dietary E/P ratio. In conclusion, recommended dietary protein and energy for rare minnow are 20%-35% and 10-12.5 kJ/g, respectively.

  20. Dietary lipid and gross energy affect protein utilization in the rare minnow Gobiocypris rarus

    Science.gov (United States)

    Wu, Benli; Xiong, Xiaoqin; Xie, Shouqi; Wang, Jianwei

    2016-07-01

    An 8-week feeding trial was conducted to detect the optimal dietary protein and energy, as well as the effects of protein to energy ratio on growth, for the rare minnow ( Gobiocypris rarus), which are critical to nutrition standardization for model fish. Twenty-four diets were formulated to contain three gross energy (10, 12.5, 15 kJ/g), four protein (20%, 25%, 30%, 35%), and two lipid levels (3%, 6%). The results showed that optimal dietary E/P was 41.7-50 kJ/g for maximum growth in juvenile rare minnows at 6% dietary crude lipid. At 3% dietary lipid, specific growth rate (SGR) increased markedly when E/P decreased from 62.5 kJ/g to 35.7 kJ/g and gross energy was 12.5 kJ/g, and from 75 kJ/g to 42.9 kJ/g when gross energy was 15.0 kJ/g. The optimal gross energy was estimated at 12.5 kJ/g and excess energy decreased food intake and growth. Dietary lipid exhibited an apparent protein-sparing effect. Optimal protein decreased from 35% to 25%-30% with an increase in dietary lipid from 3% to 6% without adversely effecting growth. Dietary lipid level affects the optimal dietary E/P ratio. In conclusion, recommended dietary protein and energy for rare minnow are 20%-35% and 10-12.5 kJ/g, respectively.

  1. Hubungan Kadar FT4 dan TSH Serum dengan Profil Lipid Darah pada Pasien Hipertiroid yang Dirawat Inap di RSUP Dr. M. Djamil Padang Tahun 2009 - 2013

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    Aga Pratama

    2014-01-01

    Full Text Available AbstrakHipertiroid merupakan sindroma klinis yang terjadi bila jaringan terpajan dengan jumlah hormon tiroid yang berlebihan karena hiperaktivitas kelenjar tiroid. Hal tersebut akan memberikan efek spesifik terhadap metabolisme sel, termasuk metabolisme lipid. Perubahan metabolisme lipid pada hipertiroid akan menimbulkan manifestasi klinis seperti gangguan mood, peningkatan perilaku depresi, dan peningkatan perilaku agresif. Dalam diagnosis pasien hipertiroid, pemeriksaan kadar FT4 dan TSH serum menjadi tes fungsi tiroid yang tepat. Penelitian ini bertujuan untuk melihat bagaimana hubungan antara kadar FT4 dan TSH serum dengan profil lipid darah pada pasien hipertiroid. Penelitian ini menggunakan data deskriptif di Instalasi Rekam Medis RSUP dr. M. Djamil Padang pada bulan Februari 2013 sampai Juli 2013. Data yang dikumpulkan berasal dari catatan rekam medik pasien hipertiroid yang dirawat inap berjumlah 21 orang dengan teknik total sampling. Analisis bivariat digunakan untuk melihat hubungan antara kadar FT4 dan TSH serum dengan profil lipid darah. Dari sampel yang ada diperoleh data rerata profil lipid, yakni: 143,33 mg/dl (kolesterol darah total; 42,06 mg/dl (HDL; 85,45 mg/dl (LDL; dan 77,19 mg/dl (trigliserida. Berdasarkan uji korelasi regresi, terdapat korelasi negatif antara kadar FT4 dengan kadar kolesterol darah total, HDL, dan LDL, tetapi tidak terdapat korelasi antara kadar FT4 dengan trigliserida. Hubungan antara kadar TSH serum dengan kolesterol darah total dan LDL mempunyai korelasi positif, tetapi tidak terdapat korelasi antara kadar TSH serum dengan HDL dan trigliserida. Penelitian ini memperlihatkan bahwa sebagian besar profil lipid darah mempunyai korelasi dengan kadar FT4 dan TSH serum, kecuali trigliserida.Kata kunci: kadar FT4 dan TSH serum, profil lipid darah, hipertiroidAbstractHyperthyroidism is a clinical syndrome that occurs when tissues are exposed by excessive amount of thyroid hormones due to thyroid gland

  2. The effects of adrenocorticotrophic hormone and an equivalent dose of cortisol on the serum concentrations of lipids, lipoproteins, and apolipoproteins.

    Science.gov (United States)

    Berg, Anna-Lena; Rafnsson, Arnar Thor; Johannsson, Magnus; Dallongeville, Jean; Arnadottir, Margret

    2006-08-01

    Previous studies have shown a strong lipid-lowering effect of adrenocorticotrophic hormone (ACTH) in healthy individuals and in patients with different kinds of dyslipoproteinemia. The mechanism behind this effect has not been established and its direct ACTH-specific nature has been questioned. Therefore, the present study was performed. Thirty healthy young males were randomized into 3 groups of equal size: one group received ACTH1-24 1 mg IM, daily for 4 days, another group was treated with cortisol 150 mg ID (50 mg tid) daily for 4 days, whereas a control group was observed for 4 days. Fasting blood samples were collected before and after treatment or observation. The serum concentrations of cholesterol (12%, P group but not in the cortisol and control groups. The statistical workup confirmed that only ACTH had a lowering effect on the apo B-containing lipoproteins. In contrast, the results indicated conformity between the treatment groups with respect to increases in the serum apo E concentrations. There were inconsistent changes in the serum concentrations of the triglycerides, high-density lipoprotein cholesterol, apo A, and lipoprotein(a). The main results were clear: the lowering effect of ACTH on the serum concentration of apo B-containing lipoproteins could not be ascribed to cortisol. These, in combination with previous in vitro results, indicated an ACTH-specific effect. PMID:16839845

  3. Comparison between swallowing and chewing of garlic on levels of serum lipids, cyclosporine, creatinine and lipid peroxidation in Renal Transplant Recipients

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    Ghorbanihaghjo Amir

    2005-05-01

    Full Text Available Abstract Abstract Hyperlipidemia and increased degree of oxidative stress are among the important risk factors for Atherosclerosis in renal transplant recipients (RTR. The Medical treatment of hyperlipidemia in RTR because of drugs side effects has been problematic, therefore alternative methods such as using of Garlic as an effective material in cholesterol lowering and inhibition of LDL Oxidation has been noted. For evaluation of garlic effect on RTR, 50 renal transplant patients with stable renal function were selected and divided into 2 groups. They took one clove of garlic (1 gr by chewing or swallowing for two months, after one month wash-out period, they took garlic by the other route. Results indicated that although lipid profile, BUN, Cr, serum levels of cyclosporine and diastolic blood pressure did not change, Systolic blood pressure decreased from138.2 to 132.8 mmHg (p=0.001 and Malondialdehyde (MDA decreased from 2.4 to1.7 nmol/ml (p=0.009 by swallowing route, Cholesterol decreased from 205.1 to 195.3 mg/dl (p=0.03, triglyceride decreased from 195.7 to 174.8 mg/dl (p=0.008, MDA decreased from 2.5 to 1.6 nmol/ml (p=0.001, systolic blood pressure decreased from 137.5 to 129.8 mmHg (p=0.001, diastolic blood pressure decreased from 84.6 to 77.6 mmHg (p=0.001 and Cr decreased from 1.51 to 1.44 mg/dl (p=0.03 by chewing route too. However HDL, LDL and cyclosporine serum levels had no significant differences by both of swallowing and chewing routes. We conclude that undamaged garlic (swallowed had no lowering effect on lipid level of serum. But Crushed garlic (chewed reduces cholesterol, triglyceride, MDA and blood pressure. Additionally creatinine reduced without notable decrease in cyclosporine serum levels may be due to cyclosporine nephrotoxicity ameliorating effect of garlic.

  4. Insulin resistance and its relation to inflammatory status and serum lipids among young women with PCOS

    OpenAIRE

    Asmathulla S; Rupa Vani K; Kripa S; Rajarajeswari R.

    2013-01-01

    Background: The incidence of polycystic ovarian syndrome (PCOS) is increasing among young women. PCOS women have decreased insulin sensitivity independent of body mass index with increase in lipid levels. Studies on measuring inflammatory status in PCOS showed varying results. The inter-relationship between inflammatory status, insulin resistance and lipid levels among PCOS women was studied. Methods: Twenty PCOS women and 20 healthy controls of age 18-25 years were recruited. Fasting blood s...

  5. Influence of genetic polymorphisms of glutathione S-transferases T1 and M1 on serum lipid parameters

    International Nuclear Information System (INIS)

    To determine the effect of genetic polymorphisms of glutathione S-transferase theta1 (GSTT1) and GSTM1 on serum levels of lipid parameters. We conducted this cross-sectional study on 152 adult healthy subjects (54 females and 98 males) from January 2004 to September 2004. The participants in our study were recruited from the Research Clinic in Abarku (Yazd province, central part of Iran). There were unrelated Iranian Muslims. The genotypes of GSTT1 and GSTM1 were determined using polymerase chain reaction based method. After an overnight fasting, serum lipid indices including triglyceride (TG), total cholesterol (TC) and high density lipoprotein cholesterol (HDL-C) were measured. There were significant partial correlation coefficients between levels of TG (r= -0.48333, df=48, p<0.0001) and TG/HDL-C ratio (r=-0.4041, df=48, p=0.004) and numbers of active GST GST genotypes in females after controlling for age and body mass index (BMI). In males, the level of TG increased as a function of numbers of active GST genotypes after controlling for age and BMI (r=+0.2082, df=94, p=0.042). There were significant differences between females and males. Data show that genetic polymorphisms of GSTM1 and GSTTT1 modulate levels of TG and TG/HDL-C in females. (author)

  6. Acute Effect of Fansidar and Antioxidant Vitamin C Co- administration on Serum Lipid profile of Wistar Albino Rats

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    Dasofunjo Kayode

    2014-10-01

    Full Text Available Purpose: Assessment of the lipid profile of fansidar and antioxidant Vitamin C co-administration in albino wistar rats.Methodology: Thirty (30 Wistar albino rats ranging from 175-200g were randomly assigned into six (6 study groups of five (5 rats each Viz: I, II, III, IV, V and VI of ten rats per group. Group I served as male control group. Group II served as female control group. Group III served as male fansidar alone treated group. Group IV served as female fansidar alone treated group. Group V served as male fansidar and Vitamin C treated group while group VI served as female fansidar and vitamin C treated group. Each rat was housed in a wooden cage. The animal room was ventilated and kept at room temperature and relative humidity of 29°c and 40-70% respectively with 12 hours natural light-dark cycle and were allowed free access to food and water ad libitum. Good hygiene was maintained by constant cleaning and removal of faeces and spilled from cages daily. Rats in all groups were weighed daily and sacrificed 24hours after the experimental periods of 14 days of oral administration and the serum were collected for lipid profile determination. Results: The body weight parameters of both male and female albino rats, showed significant increase (P<0.05 in both the control groups and fansidar + Vitamin C treated group when compared with their initial weights while the group treated with fansidar alone, showed a significant decrease (P<0.05 in weight when compared with the initial weight for both genders. Likewise, the fansidar treated groups showed a significant increase (P<0.05 in serum cholesterol when compared with the control while the fansidar + Vitamin C treated group showed no significant difference in total serum cholesterol when compared with the control. The group treated with fansidar alone showed a significant decrease in serum HDL when compared with the control while the group treated with fansidar + Vitamin C showed a significant

  7. Dietary ɛ-Polylysine Decreased Serum and Liver Lipid Contents by Enhancing Fecal Lipid Excretion Irrespective of Increased Hepatic Fatty Acid Biosynthesis-Related Enzymes Activities in Rats.

    Science.gov (United States)

    Hosomi, Ryota; Yamamoto, Daiki; Otsuka, Ren; Nishiyama, Toshimasa; Yoshida, Munehiro; Fukunaga, Kenji

    2015-03-01

    ɛ-Polylysine (EPL) is used as a natural preservative in food. However, few studies have been conducted to assess the beneficial functions of dietary EPL. The purpose of this study was to elucidate the mechanism underlying the inhibition of neutral and acidic sterol absorption and hepatic enzyme activity-related fatty acid biosynthesis following EPL intake. EPL digest prepared using an in vitro digestion model had lower lipase activity and micellar lipid solubility and higher bile acid binding capacity than casein digest. Male Wistar rats were fed an AIN-93G diet containing 1% (wt/wt) EPL or l-lysine. After 4 weeks of feeding these diets, the marked decrease in serum and liver triacylglycerol contents by the EPL diet was partly attributed to increased fecal fatty acid excretion. The activities of hepatic acetyl-coenzyme A carboxylase and glucose-6-phosphate dehydrogenase, which are key enzymes of fatty acid biosynthesis, were enhanced in rats fed EPL diet. The increased fatty acid biosynthesis activity due to dietary EPL may be prevented by the enhancement of fecal fatty acid excretion. The hypocholesterolemic effect of EPL was mediated by increased fecal neutral and acidic sterol excretions due to the EPL digest suppressing micellar lipid solubility and high bile acid binding capacity. These results show that dietary EPL has beneficial effects that could help prevent lifestyle-related diseases such as hyperlipidemia and atherosclerosis. PMID:25866749

  8. Influence of L-Carnitine Supplementation on Serum Lipid Profile in Hemodialysis Patients: A Systematic Review and Meta-Analysis

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    Haohai Huang

    2014-02-01

    Full Text Available Background/Aims: An increasing body of evidence demonstrates that L-carnitine plays a pivotal role in lipid metabolism of hemodialysis (HD patients. However, there are still some reservations about its benefits. Therefore, we performed a meta-analysis to assess the effects of L-carnitine supplementation on lipid profile in HD patients. Methods: Literature search was performed to identify the relevant randomized controlled trials that investigated the effects of L-carnitine on the lipid profile of subjects. Two independent authors used an Excel file to extract data and assess trials quality. The primary effect measure was the difference in means of the final lipid measurements between the intervention and control groups. The meta-analysis was performed with the fixed-effects model or random-effects model according to heterogeneity. Results: Twelve studies with a total of 391 patients met the inclusion criteria. The use of L-carnitine was not associated with a reduction in the total cholesterol (SMD, -0.11; 95% CI, -0.31 to 0.09, HDL-cholesterol (SMD, 0.01; 95% CI, -0.36 to 0.39, VLDL-cholesterol (SMD, 0.54; 95% CI, -0.06 to 1.14, and the serum triglycerides (SMD, -0.12; 95% CI, -0.36 to 0.12. However, L-carnitine can significantly decrease the LDL-cholesterol (SMD, -0.29; 95% CI, -0.53 to -0.06 in HD patients. In a subgroup meta-analysis, a significant LDL-cholesterol-lowering effect of L-carnitine supplementation was observed in intravenous application group, and patients with longer interventional duration and renal diseases. Conclusion: The limited evidence suggests that there was no effect of L-carnitine on serum total cholesterol, HDL-cholesterol, VLDL-cholesterol and serum triglycerides. By contrast, this meta-analysis suggests a promising effect of L-carnitine on LDL-cholesterol. Further large-scale, well-designed randomized controlled trials are urgently needed

  9. Migratory refueling affects non-enzymatic antioxidant capacity, but does not increase lipid peroxidation.

    Science.gov (United States)

    Eikenaar, Cas; Jönsson, Johanna; Fritzsch, Anna; Wang, Hong-Lei; Isaksson, Caroline

    2016-05-01

    All aerobic organisms are to some degree affected by oxidative stress, which is an imbalance between pro-oxidants and antioxidants in favor of the former. Pro-oxidants can damage DNA, proteins and lipids, and as such oxidative stress can carry considerably fitness costs. In mammals excessive calorie intake is a known cause of oxidative stress. We investigated whether in migrant birds, which typically engage in over-eating in between flights (refueling), high food intake causes oxidative stress. In an experiment we compared levels of plasmatic total non-enzymatic antioxidant capacity (AOX) and oxidative damage (lipid peroxidation) between migrants repeatedly fasted and refed (simulating the flight-refuel cycle of wild migrants), and migrants on ad libitum food. We found that refueling increased AOX, an effect mainly attributable to an increase in uric acid level, an antioxidant that is produced during protein metabolism. Accordingly, variation in AOX was mainly explained by the refueling birds' food intake. However, food intake in migrants on ad libitum food did not explain any variation in AOX. Refueling did not affect lipid peroxidation, nor were its levels explained by food intake. We propose that over-eating migrants retain uric acid, which might be a very low cost mechanism to forego oxidative damage. PMID:26921098

  10. Serum nitric oxide is associated with the risk of chronic kidney disease in women: Tehran lipid and glucose study.

    Science.gov (United States)

    Bahadoran, Zahra; Mirmiran, Parvin; Tahmasebi Nejad, Zhale; Ghasemi, Asghar; Azizi, Fereidoun

    2016-07-01

    Background and aim This study was conducted to investigate the association between serum nitric oxide metabolites (NOx) and the risk of chronic kidney disease (CKD). Methods We recruited 3462 and 2504 participants of the Tehran Lipid and Glucose Study (TLGS), for a cross-sectional and prospective analysis, respectively. Serum NOx concentrations were measured at baseline (2006-2008), and demographics, anthropometrics and biochemical variables were evaluated at baseline and again after 3 years (2009-2011). Estimated glomerular filtration rate (eGFR) and CKD were defined. Association between serum NOx and CKD in the cross-sectional phase and the predictability of NOx in CKD occurrence were assessed using multivariable logistic regression models with adjustment for confounders. Results Mean age of participants was 45.0 ± 15.9 years at baseline and 40.5% were male. The prevalence of CKD was 17.9% (13.4% in men, 21.0% in women) at baseline, at which point, marginally significant odds of CKD in the highest tertile of serum NOx in men (OR = 1.53, 95% CI = 0.96-2.45, p for trend = 0.047) and a significant odds of CKD in women (OR = 2.48, 95% CI = 1.76-3.49, p for trend = 0.001) were observed. After a 3-year follow-up, in women, risk of CKD was higher in the highest compared to the lowest NOx tertiles (OR = 1.86, 95% CI = 1.10-3.14, p for trend = 0.032) but no significant association was observed in men. Conclusion Serum NOx level was found to be an independent predictor of CKD in women; it could be a valuable surrogate for prediction of renal dysfunction in women and help to identify high-risk subjects. PMID:26956540

  11. Effects of Combined Selective Aerobic Moderate Intensity Exercises and Soya Intake on Serum Lipids and Obesity in Obese Postmenopausal Women

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    A Zarneshan

    2012-02-01

    Full Text Available Background & Aim: Today, the percentage of deaths due to heart diseases has increased and along with the exercise training role in reducing obesity and cardiovascular disease, soy is a useful source of food in reducing blood lipid and obesity. The present study investigated the effect of combined selective short aerobic moderate intensity exercise and soya intake on serum lipids and obesity in obese postmenopausal women. Methods: In the present quasi-experimental study conducted in 2006 on women of Urmia, Iran, 56 obese postmenopausal women were selected and randomly divided into four groups of 14 subjects as follow: exercise-soya, exercise, soya, and control group. Pre- and post protocol blood samples were collected and the level of TG (Triglyceride, tCho (Total Cholesterol, LDL-C (Low density lipoprotein, HDL-C (high density lipoprotein were measured. Exercise training within the range of 60-70% maximum heart rate (MHR was performed for 60 minutes, 3 sessions per week. The subjects of soya group and exercise-soya group had a 100 gram soya nut intake daily for 10 weeks. After The training course, blood samples were taken from the subjects. The collected data was analyzed using Two-way ANOVA and paired t-test. Results: Having soya along with exercise had significant impact on reduction of TG, tCho, LDL-C means (p<0.05. Body mass index and waist to hip ratio decreased significantly in exercise-soya group after 10 weeks (p<0.05. Conclusion: Based on the findings of this study, aerobic moderate intensity exercise along with soya intake, decrease obesity and serum lipids in obese postmenopausal women.

  12. Serum levels of mitochondrial uncoupling protein 1, leptin, and lipids during late pregnancy and the early postpartum period in mares.

    Science.gov (United States)

    Arfuso, F; Giannetto, C; Rizzo, M; Fazio, F; Giudice, E; Piccione, G

    2016-09-15

    The aim of this study was to investigate the changes of serum mitochondrial uncoupling protein 1 (UCP1) and leptin levels as well as of lipid and lipoprotein profiles in mares during the peripartum period. Ten pregnant mares (group A) were monitored from 15 ± 3 days of pregnancy until 15 days after foaling, and 10 nonpregnant nonlactating mares constituted the control (group B). In group A, blood sampling was performed on Days 15 ± 3 and 7 ± 3 before foaling, on the day of foaling, and on Days 7 and 15 after foaling. In group B, blood sampling was performed on the same days as in group A. Serum levels were determined for UCP1, leptin, total lipids, phospholipids, triglycerides, total cholesterol (Total-Chol), high-density lipoproteins, low-density lipoproteins (LDLs), and very low-density lipoproteins (VLDLs). Two-way repeated-measures ANOVA was applied to evaluate the effects of peripartum period and group membership. All studied parameters except phospholipid levels (P > 0.05) showed significant changes in group A over the peripartum period (P leptin and total lipids, triglycerides, VLDLs, Total-Chol, and LDLs. These changes observed in mares during the peripartum period could represent a response to hormonal and metabolic adaptations occurring during specific physiological conditions such as late pregnancy and early postpartum. These changes should compensate for the energy loss occurring during these particular life phases and ensure a good body condition to protect mares against negative energy balance. PMID:27165993

  13. COMPARATIVE STUDY OF SERUM LIPID PROFILE IN OBESE TYPE 2 DIABETIC PATIENTS WITH OBESE NON DIABETIC PATIENTS

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    Jain

    2014-11-01

    Full Text Available Diabetes is a group of metabolic diseases characterized by high blood glucose levels while obesity is accumulation of excess body fat. Diabetes and obesity currently threaten the health, wellbeing and economic welfare of humanity OBJECTIVE: To study serum lipid profiles in Obese type 2 diabetics in comparison with Obese non-diabetic subjects. MATERIALS AND METHODS: The study was conducted in the Department of Biochemistry in collaboration with Department of Medicine, Sri Aurobindo Medical College & Post Graduate Institute, Indore. In this study a total of 200 subjects included, out of which 100 type 2 diabetic patients with obesity (BMI ≥30 kg/m² taken as cases and 100 obese patients without diabetes mellitus are taken as controls as per the criteria of study design and their serum lipid profile was analyzed & computed. RESULTS: Majority of the study population were males in both cases and controls. No significant differences were observed between different age categories between cases and controls. In cases the mean TC levels, TG levels, LDLc levels, VLDLc levels found to be 225.95±24.51mg%, 175.35±24.45 mg%, 170.77±18.86 mg%, 35.03±4.89 mg% and it was found to be higher than controls. However, the mean HDLc value and the HDLc /LDLc ratio was found to be 37.03±7.84 mg% and 0.44±0.25 which was higher than those reported in the cases. A significant difference (P<0.0001 was found between cases and control population when the serum TC, TG, HDLc values, LDLc values, VLDLc values, HDLc /LDLc ratio were compared between them. CONCLUSION: Dyslipidemia is more in obese diabetics than obese non diabetics and it can be prevented with proper monitoring and therapy to avoid associated comorbidity of diabetes mellitus.

  14. Association of fasting plasma glucose and serum lipids in Type 2 diabetics

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    S Khadke

    2015-01-01

    Full Text Available Dyslipidemia is a significant morbidity associated with diabetes and cardiovascular disorders. The present study was undertaken to assess the lipid profile of type 2 diabetic and age-gender matched healthy subjects and its association, if any, with fasting plasma glucose. Clinically diagnosed diabetic subjects were recruited for the study. The fasting plasma glucose and lipid profiles were analyzed for 99 diabetic and 101 healthy volunteers. The blood samples were analyzed for fasting plasma glucose, total cholesterol, triglycerides, high density lipoprotein-cholesterol, low density lipoprotein-cholesterol and very low density lipoprotein-cholesterol. Correlation analysis of lipid profile with fasting plasma glucose and calculation of risk ratio was done. The levels of high density lipoprotein-cholesterol and low density lipoprotein-cholesterol were found to be significantly low in diabetics and subjects with lower low density lipoprotein-cholesterol were on statins. Inspite of lower lipid values, the risk ratio for diabetics was significantly higher. The correlation analysis indicated significant difference in relationship between fasting plasma glucose, lipid parameters and risk ratios in the two groups. Diabetics with lower high density lipoprotein-cholesterol and higher total cholesterol present with a higher risk ratio pointing to need of non-statin high density lipoprotein-raising medications decreasing their predisposition to cardiovascular disorders. The study highlights the altered pattern of correlation of lipid profile with fasting plasma glucose in diabetics and their increased risk of cardiovascular disorders. The dyslipidemia in the form of triglyceridemia and significantly low high density lipoprotein-cholesterol in diabetics point towards the need of non-statin high density lipoprotein-raising medications.

  15. Serum Levels of Tryptophan, 5-Hydroxytryptophan and Serotonin in Patients Affected with Different Forms of Amenorrhea

    Directory of Open Access Journals (Sweden)

    S. Comai

    2010-06-01

    Full Text Available Tryptophan (Trp is present in the serum, partly bound to albumine and in the free form. The unbound portion of circulating tryptophan has the property of crossing the hematoencephalic barrier and being converted within the brain into serotonin (5-HT through the enzymatic processes of hydroxylation and decarboxylation. The serotoninergic system plays an important role in neuroendocrine control of reproductive hormone secretion, and in particular, it may influence GnRH pulsatility, a function essential for reproductive processes. In this study, we analysed serum levels of tryptophan, serotonin and 5-hydroxytryptophan (5-HTP in women with three different forms of amenorrhea: 16 patients were diagnosed with anorexia nervosa, 60 patients with functional hypothalamic amenorrhea, and 14 patients with hyperprolactinemia. Data were compared with those of a group of 25 healthy women. Serum Trp levels were significantly (P ≤ 0.05 lower in the anorexic (11.64 ± 0.53 µg/ml, mean ± S.E. than in the control (12.98 ± 0.37 µg/ml groups. In addition, in the anorexic group a statistical dispersion of Trp values was shown indicating a bimodal data distribution suggesting the existence of two different subgroups of patients. Regarding 5-HTP, an increase of its serum level was observed in all the groups with amenorrhea with the highest value in hyperprolactinemic patients. On the contrary, no statistical differences in serum 5-HT levels among the four analyzed groups were observed. This study shows that women affected by various forms of amenorrhea present an altered metabolism of tryptophan via serotonin and, in particular, markedly high differences are observed between the two subgroups of anorexic patients.

  16. Correlation between anthropometric measurement, lipid profile, dietary vitamins, serum antioxidants, lipoprotein (a) and lipid peroxides in known cases of 345 elderly hypertensive South Asian aged 56-64 y-A hospital based study

    Institute of Scientific and Technical Information of China (English)

    Arun Kumar

    2014-01-01

    Objective: To address the association of dietary vitamins, anthropometric profile, lipid profile, antioxidant enzymes and lipid peroxidation in hypertensive participant compared with normotensive healthy controls.Methods:in both hypertensive participants and normotensive age-sex matched healthy controls. The associated changes in serum antioxidants and lipid peroxidation were also assessed along with lipid profile and anthropometric measurements in both groups of subjects under study.Results:Dietary intake of vitamins was assessed by 131 food frequency questionnaire items B2 and ascorbic acid compared to normotensive controls. Anthropometric variables in the hypertensive showed significant differences in weight, body mass index, waist circumference, hip circumference, waist-hip ratio and mid-arm circumference. The total cholesterol, low-density lipoprotein cholesterol, triglyceride were significantly higher (P<0.001) in hypertensive except high-density lipoprotein cholesterol which was significantly higher (P<0.001) in normotensive. The serum endogenous antioxidants and enzyme antioxidants were significantly decreased in hypertensive except serum albumin levels compared to normotensive along with concomitant increase in serum lipoprotein (a) malondialdehyde and conjugated diene levels. Dietary vitamins intake was higher in hypertensive participants excepting for vitamin Conclusions: Based on the observations, our study concludes that hypertension is caused due to interplay of several confounding factors namely anthropometry, lipid profile, depletion of endogenous antioxidants and rise in oxidative stress.

  17. IN SEARCH OF THE MISSING LINK: SERUM LIPID PROFILE, TROPONIN T AND ACUTE CORONARY SYNDROME.

    Directory of Open Access Journals (Sweden)

    Basabdatta Samanta

    2014-01-01

    Full Text Available Acute coronary syndrome is one of the leading causes of morbidity and mortality worldwide , hyperlipidemias being a major predisposing factor. Cardiac Troponin T (cTnT is one of the most sensitive and specific biomarkers of myocardial injury. The aim of the study was to evaluate the relationship among TnT levels and lipid profiles of different age groups of patients with ACS , and to determine if any the association of age with lipid profile and TnT levels. The lipid profiles and TnT levels were de termined in 464 patients with acute coronary syndrome. Depending upon age , the patients were divided into groups 1 , 2 and 3 (49 ≤ , 50 - 69 and ≤ 70 years respectively. Increasing age was found to be associated with fall in mean levels of total cholesterol , LD L , triglycerides and TnT. Our study suggested that among patients with ACS , there exists a relation between age , lipid components and TnT levels. TnT is known to be an independent predictor of the outcome of myocardial ischemia. Further studies are therefo re required to ascertain the validity of TnT as a prognostic cardiac biomarker among the elderly

  18. Serum insensitive, intranuclear protein delivery by the multipurpose cationic lipid SAINT-2

    NARCIS (Netherlands)

    van der Gun, Bemardina T. F.; Monami, Amlie; Laarmann, Sven; Rasko, Tamas; Slaska-Kiss, Krystyna; Weinhold, Elmar; Wasserkort, Reinhold; de Leij, Lou F. M. H.; Ruiters, Marcel H. J.; Kiss, Antal; McLaughlin, Pamela M. J.

    2007-01-01

    Cationic liposomal compounds are widely used to introduce DNA and siRNA into viable cells, but none of these compounds are also capable of introducing proteins. Here we describe the use of a cationic amphiphilic lipid SAINT-2:DOPE for the efficient delivery of proteins into cells (profection). Label

  19. Preventive Effect of Berberis Integerrima on the Serum Levels of Glucose and Lipids in Streptozotocin (STZ)-Induced Diabetes in Rats

    OpenAIRE

    Hossein Ashraf; Reza Heydari; Vahid Nejati; Minoo Ilkhanipoor

    2012-01-01

    Background and Objective: Use of medicinal plants for attenuation of hyperglycemia and restoration of lipids to normal level is clinically very important. We decided to assess the preventive role of aqueous extract of Berberis Integerrima root on the serum levels of glucose, insulin, and lipid profile in Streptozotocin (STZ)-induced diabetic rats.Materials and Methods: Forty male rats were divided into 5 groups as follows: 1- normal (N); 2- normal + barberry (N+B) (they received barberry root...

  20. Effect of prebiotic fibre supplementation on hepatic gene expression and serum lipids: a dose–response study in JCR:LA-cp rats

    OpenAIRE

    Parnell, Jill A.; Reimer, Raylene A.

    2009-01-01

    Prebiotic fibres have been proposed to promote weight loss and lower serum cholesterol; however, the mechanisms are not fully understood. The aim of the present research was to identify possible mechanisms through which prebiotic fibres improve serum lipids. Lean and obese JCR:La-cp rats aged 8 weeks consumed one of three diets supplemented with 0, 10 or 20 % prebiotic fibre for 10 weeks. Rats were anaesthetised and a fasting blood sample was taken for lipid analysis. Real-time PCR was used t...

  1. Study on changes of serum lipid profile after withdrawl of levo-thyroxine replacement therapy in patients with radical thyroidectomy for cancer

    International Nuclear Information System (INIS)

    Objective: To study the changes of serum lipid profile after withdrawl of levo-thyroxine replacement for 4 weeks in patient with radical thyroidectomy for cancer. Methods: Serum thyroid hormones (FT3, FT4, sTSH, with RIA) levels and lipid profile (Total cholesterol, triglycerides, LDL, HDL, apolipoprotein A-I, apolipoprotein B and Lp (a), with biochemistry) were determined in 46 patients with thyroid carcinoma after operation both during and after 4 weeks' withdrawl of levo-thyroxine replacement treatment. Results: Serum FT3, FT4 levels decreased significantly and serum sTSH, TC, TG, LDL, apo-B, Lp (a) levels increased significantly after 4 weeks' withdrawl of levo-thyroxine replacement (vs during treatment, P 0.05). Conclusion: Hyperlipidaemia did develop in the hypothyroid patients after withdrawl of levo-thyroxine replacement but the degree of which did not not necessarily correlate with the increment of sTSH levels. (authors)

  2. [Effect of shengmaisan on serum lipid peroxidation in acute viral myocarditis].

    Science.gov (United States)

    Zhao, M H; Rong, Y Z; Lu, B J

    1996-03-01

    The effect of Shengmaisan (SMS) on 62 acute viral myocarditis patients and its peroxidation damage was studied. The results revealed that the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in blood were decreased and the content of malondialdehyde (MDA) in plasma was increased in acute viral myocarditis patients in comparison with the healthy controls (P SMS group and placebo group. After treatment, both SOD and GSH-Px activities were increased and the level of MDA decreased (P SMS group, while those in placebo group were not changed (P < 0.05). The results suggested that the myocardial damage of viral myocarditis is closely related with lipid peroxidation SMS acts as an effective free radical scavenger and anti-lipid peroxidation drug. SMS could prevent the damage of myocardia and might be taken as one of the effective therapeutic methods in treatment of acute viral myocarditis. PMID:9208534

  3. Effects of Orange Juice and Hesperetin on Serum Paraoxonase Activity and Lipid Profile in Hyperuricemic Rats

    OpenAIRE

    Fatemeh Haidari; Mohammad-Reza Rashidi; Majid Mohammad-Shahi

    2012-01-01

    Introduction: Hypouricemic, antioxidant and xanthine oxidase inhibitory effects of orange juice and hesperetin have been already indicated. The objective of this study was to investigate the effects of orange juice and hesperetin on paraoxonase and arylesterase activity and lipid profile of hyperuricemic rats. Methods: Forty eight male Wistar rats were divided into 8 equal groups of healthy control, healthy+orange juice, healthy+hesperetin, healthy+allopurinol, hyperuricemic control, hyperuri...

  4. A High Legume Low Glycemic Index Diet Improves Serum Lipid Profiles in Men

    OpenAIRE

    Zhang, Zhiying; Lanza, Elaine; Kris-Etherton, Penny M.; Colburn, Nancy H.; Bagshaw, Deborah; Rovine, Michael J.; Ulbrecht, Jan S.; Bobe, Gerd; Chapkin, Robert S.; Hartman, Terryl J.

    2010-01-01

    Clinical studies have shown that fiber consumption facilitates weight loss and improves lipid profiles; however, the beneficial effects of high fermentable fiber low glycemic index (GI) diets under conditions of weight maintenance are unclear. In the Legume Inflammation Feeding Experiment, a randomized controlled cross-over feeding study, 64 middle-aged men who had undergone colonoscopies within the previous 2 years received both a healthy American (HA) diet (no legume consumption, fiber cons...

  5. The impact of serum lipids on risk for microangiopathy in patients with type 2 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Toth Peter P

    2012-09-01

    Full Text Available Abstract Background Few large-scale, real-world studies have assessed the relative associations of lipid fractions with diabetic microvascular events. The main objective of this study was to evaluate the association of the lipid profile components, high density lipoprotein cholesterol (HDL-C, low density lipoprotein cholesterol (LDL-C, triglycerides (TG, and non-high density lipoprotein cholesterol (non-HDL-C with microvascular complications (MVCs in type 2 diabetes mellitus (T2DM patients. Methods This observational cohort study queried the HealthCore Integrated Research Database (HIRDSM for newly-diagnosed (Index Date 18-64-year-old patients with diabetes mellitus between 01/01/2005-06/30/2010. Inclusion required ≥12 months pre-index continuous health plan eligibility and ≥1 pre-index lipid profile result. Patients with polycystic ovary syndrome and prior MVCs were excluded. Incident complications were defined as the earliest occurrence of diabetic retinopathy, peripheral neuropathy, and/or nephropathy post-index. Cox proportional models and Kaplan-Meier (KM curves were used to evaluate associations among variables. Results Of the patients (N = 72,267, 50.05 % achieved HDL-C, 64.28 % LDL-C, 59.82 % TG, and 56.79 % non-HDL-C American Diabetes Association goals at baseline. During follow-up (mean, 21.74 months, there were 5.21 microvascular events per 1,000 patient-months. A 1-mg/dL increase in HDL-C was associated with 1 % decrease in any MVC risk (P P P P P P  Conclusion This study demonstrates significant independent associations among lipid fractions and risk for microangiopathy. These findings suggest that attaining established ADA goals for HDL-C, TG, and non-HDL-C may reduce risk for microvascular events among patients with diabetes.

  6. Effect of a phytogenic feed additive on performance, ovarian morphology, serum lipid parameters and egg sensory quality in laying hen

    OpenAIRE

    Saki, Ali Asghar; Aliarabi, Hassan; Hosseini Siyar, Sayed Ali; Salari, Jalal; Hashemi, Mahdi

    2014-01-01

    This present study was conducted to evaluate the effects of dietary inclusion of 4, 8 and 12 g kg-1 phytogenic feed additives mixture on performance, egg quality, ovary parameters, serum biochemical parameters and yolk trimethylamine level in laying hens. The results of experiment have shown that egg weight was increased by supplementation of 12 g kg-1 feed additive whereas egg production, feed intake and feed conversion ratio (FCR) were not significantly affected. There were no significant d...

  7. Effect of a MAST Exercise Program on Anthropometric Parameters, Physical Fitness, and Serum Lipid Levels in Obese Postmenopausal Women

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    Trabka Bartosz

    2014-10-01

    Full Text Available The purpose of this study was to examine an influence of a mixed aerobic and strength training program (MAST on anthropometry, serum lipid levels, physical performance, and functional fitness in obese postmenopausal women. The MAST sessions were held three times per week, and the exercise program lasted for 10 weeks. The exercise group demonstrated a statistically significant improvement in maximal oxygen uptake, a waist/hip ratio, and strength of the upper and lower body. An increase in LDL-C levels was observed in the control group. A 10-week MAST program encompassing Nordic-walking as an aerobic component, and strength exercises, induces positive changes in functional fitness, HDL-C, LDL-C and a waist/hip ratio in obese postmenopausal women. The observed changes implicate an increase in a health-related quality of life among the women administered to the physical exercise program

  8. Human serum albumin supported lipid patterns for the targeted recognition of microspheres coated by membrane based on ss-DNA hybridization

    International Nuclear Information System (INIS)

    Human serum albumin (HSA) patterns have been successfully fabricated for the deposition of lipid bilayer, 1,2-dimyristoyl-sglycerophosphate (DMPA), by making use of the micro-contact printing (μCP) technique and liposome fusion. Confocal laser scanning microscopy (CLSM) results indicate that lipid bilayer has been assembled in HSA patterns with a good stability. Such well-defined lipid patterns formed on HSA surface create possibility to incorporate specific components like channels or receptors for specific recognition. In view of this, microspheres coated with lipid membranes were immobilized in HSA-supported lipid patterns via the hybridization of complementary ss-DNAs. This procedure enables to transfer solid materials to a soft surface through a specific recognition

  9. Distribution of serum lipids and lipoproteins in patients with beta thalassaemia major; an epidemiological study in young adults from Greece

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    Barbetseas John

    2004-03-01

    Full Text Available Abstract Background Beta-thalassaemia major (b-TM has been defined as a combination of chronic hemolytic anemia, iron storage disease and myocarditis, and it has been associated with premature death especially due to heart failure. To the best of our knowledge the status of blood lipids in these patients has rarely been investigated. Thus, we assessed the levels of lipids and lipoproteins in a sample of cardiovascular disease free adult men and women with b-TM. Methods During 2003 we enrolled 192 consecutive patients with b-TM that visited our Institution for routine examinations. The Institution is considered the major reference center for b-TM in Greece. Of the 192 patients, 88 were men (25 ± 6 years old and 104 women (26 ± 6 years old. Fasting blood lipid levels were measured in all participants. Results Data analysis revealed that 4% of men and 2% of women had total serum cholesterol levels > 200 mg/dl, and 11% of men and 17% of women had triglyceride levels > 150 mg/dl. In addition, mean HDL cholesterol levels were 32 ± 11 mg/dl in men and 38 ± 10 mg/dl in women, lipoprotein-a levels were 8.3 ± 9 mg/dl in men and 8.8 ± 9 mg/dl in women, apolipoprotein-A1 levels were 111 ± 17 mg/dl in men and 123 ± 29 mg/dl in women, and apolipoprotein-B levels were 60 ± 20 mg/dl in men and 59 ± 14 mg/dl in women. Total-to-HDL cholesterol ratios were 3.7 ± 1.2 and 3.8 ± 1.5 in men and women, respectively. Conclusions The majority of the patients had blood lipid levels (by the exception of HDL-cholesterol within the normal range, and consequently the prevalence of lipid and lipoprotein abnormalities was much lower as compared to the general population of the same age. Interestingly, is that the total – to HDL cholesterol ratio was high in our patients, and may underline the importance of this index for the prognosis of future cardiac events in these patients.

  10. SERUM LIPID PROFILE OF SPAYED AND NON-SPAYED FEMALE DOGS ASSOCIATED WITH THE BODY CONDITION SCORE

    Directory of Open Access Journals (Sweden)

    Patricia Almeida Ferreira

    2015-04-01

    Full Text Available Neutering females may be associated to weight gain in dogs. In order to diagnose possible alterations, such as diabetes and hyperlipidemias, laboratory assessment of carbohydrate and lipid metabolism is important. The aim of this study was to verify glycemia and serum lipid profile in non-spayed and spayed female dogs, and associate these results with the body condition score. Thirty-two spayed (n=16 and non-spayed (n=16 female dogs had their blood collected for biochemical analysis, and were classified as to their body condition score (BCS. Nine had normal BCS, 10 were overweight, and 13 were obese. Little difference was observed among spayed and non-spayed dogs. Glycemia of spayed canine females is higher than that of non-spayed dogs, regardless of the body condition score. Likewise, total cholesterol levels are higher in non-spayed females. The HDL is higher in non-spayed obese dogs than in spayed dogs with the same body condition, this parameter did not differ in other weight groups. Contrary to what happens in post-menopausal women, spaying dogs does not seem to have such a strong effect on the metabolic parameters assessed. This study revealed unique results, where neither spaying nor weight group had a strong association with metabolic alterations.

  11. Kiwifruit consumption favourably affects plasma lipids in a randomised controlled trial in hypercholesterolaemic men.

    Science.gov (United States)

    Gammon, Cheryl S; Kruger, Rozanne; Minihane, Anne M; Conlon, Cathryn A; von Hurst, Pamela R; Stonehouse, Welma

    2013-06-28

    The unique composition of green kiwifruit has the potential to benefit CVD risk. The aim of the present study was to investigate the effect of consuming two green kiwifruits daily in conjunction with a healthy diet on plasma lipids and other metabolic markers and to examine response according to APOE genotype in hypercholesterolaemic men. After undergoing a 4-week healthy diet, eighty-five hypercholesterolaemic men (LDL-cholesterol (LDL-C) > 3.0 mmol/l and TAG < 3 mmol/l) completed an 8-week randomised controlled cross-over study of two 4-week intervention sequences of two green kiwifruits per d plus healthy diet (intervention) or healthy diet alone (control). Anthropometric measures, blood pressure (BP) and fasting blood samples (plasma lipids, serum apoA1 and apoB, insulin, glucose, high-sensitivity C-reactive protein (hs-CRP)) were taken at baseline, and at 4 and 8 weeks. After the kiwifruit intervention, plasma HDL-cholesterol (HDL-C) concentrations were significantly higher (mean difference 0.04; 95% CI 0.01, 0.07 mmol/l; P = 0.004) and the total cholesterol (TC):HDL-C ratio was significantly lower (mean difference 20.5; 95% CI 20.24, 20.05 mmol/l; P = 0.002) compared with the control. In carriers of the APOE4 allele, TAG decreased significantly (mean difference -0.18; 95% CI -0.34, -0.02 mol/l; P = 0.03) with kiwifruit compared with control. There were no significant differences between the two interventions for plasma TC, LDL-C, insulin, glucose, hs-CRP and BP. The small but significant increase in HDL-C and decrease in TC:HDL-C ratio and TAG (in APOE4 carriers) suggest that the regular inclusion of green kiwifruit as part of a healthy diet may be beneficial in improving the lipid profiles of men with high cholesterol. PMID:23151354

  12. Salmon Protamine Decreases Serum and Liver Lipid Contents by Inhibiting Lipid Absorption in an In Vitro Gastrointestinal Digestion Model and in Rats.

    Science.gov (United States)

    Hosomi, Ryota; Miyauchi, Kazumasa; Yamamoto, Daiki; Arai, Hirofumi; Nishiyama, Toshimasa; Yoshida, Munehiro; Fukunaga, Kenji

    2015-10-01

    Protamine has been used as an antiheparin drug and a natural preservative in various food products. However, limited studies have evaluated the physicochemical and functional properties of protamine. Hence, we assessed the mechanisms underlying the inhibition of lipid absorption following salmon protamine intake in in vitro and in vivo studies. In initial experiments, a salmon protamine hydrolyzate (PH) was prepared using in vitro simulated gastrointestinal digestion suppressed pancreatic lipase activity and micellar cholesterol solubility. This PH had higher bile acid-binding capacity and emulsion breakdown activity than casein hydrolyzate and l-arginine. However, the hypolipidemic functions of protamine were dramatically reduced by pancreatin digestion. In further experiments, groups of male Wistar rats were fed an AIN-93G diet containing 5% (wt/wt) salmon protamine or a protamine amino acid mixture. After 4 wk of feeding with experimental diets, reductions in serum and liver triacylglycerol (TAG) and cholesterol contents were observed in the presence of protamine, reflecting inhibition of TAG, cholesterol, and bile acid absorption. These data suggest that the formation of insoluble PH-bile acid complexes is critical before the bile acid-binding capacity is reduced. Therefore, dietary salmon protamine may ameliorate lifestyle-related diseases such as hyperlipidemia and obesity. PMID:26352573

  13. Hydrocortisone stimulates the development of oligodendrocytes in primary glial cultures and affects glucose metabolism and lipid synthesis in these cultures

    OpenAIRE

    Warringa, R.A.J.; Hoeben, R C; Koper, W.J.; Sykes, J.E.C.; Golde, L.M.G. van; Lopes-Cardozo, M.

    1987-01-01

    Cultures of glial cells were prepared from the brains of one-week-old rat pups. After one day in culture, serum was omitted from the medium and replaced by a combination of growth-stimulating hormones and other factors that enhanced the percentage of oligodendrocytes in the cultures. We investigated the effects of hydrocortisone on the development of oligodendrocytes, on the activities of oligodendrocyte-specific enzymes and on glucose- and lipid-metabolism of the glial cells. (1) Hydrocortis...

  14. Tragopogon porrifolius improves serum lipid profile and increases short-term satiety in rats.

    Science.gov (United States)

    Zeeni, Nadine; Daher, Costantine F; Saab, Lea; Mroueh, Mohamad

    2014-01-01

    Tragopogon porrifolius (white salsify) is an edible plant commonly used in folk medicine in Lebanon and neighbouring countries. This study investigates the effect of the aqueous extract of the aerial part of T. porrifolius on lipemia and appetite regulation using a rat model. Food intake, abdominal fat percentage, blood lipid profile, liver weight and liver enzymes were assessed following 4 weeks of extract intake via drinking water (50, 100, or 250 mg/kg body weight) in standard high-carbohydrate and high-fat dietary conditions. In a separate study, the short term effect of a preload of T. porrifolius extract on food intake was evaluated. Results showed that consumption of the plant extract for a period of four weeks resulted in a marked improvement of the lipid profile (triglycerides, total cholesterol, LDL and HDL cholesterol). Body weight, food intake and intra-abdominal fat were also lower in animals given the plant extract (100 and 250 mg/kg). In addition, T. porrifolius extract preload produced a dose dependent decrease in food intake observed over 24h. The intake of T. porrifolius aqueous extract therefore improved lipemia and increased satiety in rats with no visible adverse effects. PMID:24099703

  15. Varying plant protein sources in the diet of sea bass Dicentrarchus labrax differently affects lipid metabolism and deposition

    Directory of Open Access Journals (Sweden)

    E. Tibaldi

    2010-04-01

    Full Text Available The liver activity of lipogenic enzymes, the lipid content in various tissues, and plasma lipid levels of major, were measured in sea bass (D. labrax fed over 96 days either a, fish meal-based control diet or preparations where 70% of fish meal protein was replaced by wheat gluten singly or in combination with pea or soybean meals. Relative to the controls, sea bass fed the wheat gluten-based diet resulted in stimulated lipogenesis in liver and increased lipid deposition in muscle. The opposite occurred when a substantial amount of soybean meal was included in the diet. Mesenteric fat depots were apparently insensitive to major changes in dietary protein source in fish showing similar intakes of digestible protein, energy and lipid. These results confirm that varying plant protein source in the diet differently affects lipid metabolism and deposition in sea bass.

  16. Effect of Prebiotic Galacto-Oligosaccharides on Serum Lipid Profile of Hypercholesterolemics.

    Science.gov (United States)

    Hashmi, Arooj; Naeem, Naureen; Farooq, Zubair; Masood, Saima; Iqbal, Sanaullah; Naseer, Rahat

    2016-03-01

    The present study focused on the role of galacto-oligosaccharides (GOS) against the development of hypercholesterolemia. In the current research, GOS synthesized from lactose solution were fed to hypercholesterolemic female Sprague-Dawley rats. Negative control group (G1) was fed on standard basal diet alone. Positive control group (G5) was fed on inulin (154 mg/250 g body weight), while treatment groups G2, G3 and G4 received 110 mg, 154 mg and 198.4 mg/250 g body weight, respectively, of GOS along with high-fat diet for a period of 60 days. Findings from this study revealed that animals belonging to prebiotic GOS (G2, G3 and G4)-fed group showed significantly decreased serum triglycerides, total cholesterol, LDL cholesterol and VLDL cholesterol as compared to control group (G0). The groups which were fed on different doses of GOS revealed a significant reduction in TC, TG, LDL, and VLDL levels and an increase in HDL level corresponding to the reference group that was fed on inulin, while G1 negative control group revealed increased levels of TC, TG, LDL and VLDL. In contrast to positive control group G5 (154 mg inulin), all doses of GOS lowered serum TC, TG and LDL-C and raised HDL-C; however, G4 (198.4 mg) proved to be more effective. Hence, GOS proved to be supportive in preventing hypercholesterolemia leading cause of cardiovascular disease and atherosclerosis. This study reported a significant reduction of serum TC, TG and LDL-C in female rats for 60 days as compared to control. Conclusively, GOS were found to be worthless against hypercholesterolemia. PMID:26905736

  17. Lung inflammation does not affect the clearance kinetics of lipid nanocapsules following pulmonary administration.

    Science.gov (United States)

    Patel, Aateka; Woods, A; Riffo-Vasquez, Yanira; Babin-Morgan, Anna; Jones, Marie-Christine; Jones, Stuart; Sunassee, Kavitha; Clark, Stephen; T M de Rosales, Rafael; Page, Clive; Spina, Domenico; Forbes, Ben; Dailey, Lea Ann

    2016-08-10

    Lipid nanocapsules (LNCs) are semi-rigid spherical capsules with a triglyceride core that present a promising formulation option for the pulmonary delivery of drugs with poor aqueous solubility. Whilst the biodistribution of LNCs of different size has been studied following intravenous administration, the fate of LNCs following pulmonary delivery has not been reported. We investigated quantitatively whether lung inflammation affects the clearance of 50nm lipid nanocapsules, or is exacerbated by their pulmonary administration. Studies were conducted in mice with lipopolysaccharide-induced lung inflammation compared to healthy controls. Particle deposition and nanocapsule clearance kinetics were measured by single photon emission computed tomography/computed tomography (SPECT/CT) imaging over 48 h. A significantly lower lung dose of (111)In-LNC50 was achieved in the lipopolysaccharide (LPS)-treated animals compared with healthy controls (p<0.001). When normalised to the delivered lung dose, the clearance kinetics of (111)In-LNC50 from the lungs fit a first order model with an elimination half-life of 10.5±0.9h (R(2)=0.995) and 10.6±0.3h (R(2)=1.000) for healthy and inflamed lungs respectively (n=3). In contrast, (111)In-diethylene triamine pentaacetic acid (DTPA), a small hydrophilic molecule, was cleared rapidly from the lungs with the majority of the dose absorbed within 20min of administration. Biodistribution to lungs, stomach-intestine, liver, trachea-throat and blood at the end of the imaging period was unaltered by lung inflammation. This study demonstrated that lung clearance and whole body distribution of lipid nanocapsules were unaffected by the presence of acute lung inflammation. PMID:27180635

  18. Influence of Serum and Hypoxia on Incorporation of [(14)C]-D-Glucose or [(14)C]-L-Glutamine into Lipids and Lactate in Murine Glioblastoma Cells.

    Science.gov (United States)

    Ta, Nathan L; Seyfried, Thomas N

    2015-12-01

    Glucose and glutamine are essential energy metabolites for brain tumor growth and survival under both normoxic and hypoxic conditions. Both metabolites can contribute their carbons to lipid biosynthesis. We used uniformly labeled [(14)C]-U-D-glucose and [(14)C]-U-L-glutamine to examine the profile of de novo lipid biosynthesis in the VM-M3 murine glioblastoma cells. The major lipids synthesized included phosphatidylcholine (PtdCho), phosphatidylethanolamine (EtnGpl), phosphatidylinositol (PtdIns), phosphatidylserine (PtdSer), sphingomyelin (CerPCho), bis(monoacylglycero)phosphate (BMP)/phosphatidic acid (PtdOH), cholesterol (C), cardiolipin (Ptd2Gro), and gangliosides. Endogenous lipid synthesis, using either glucose or glutamine, was greater in media without fetal bovine serum (FBS) than in media containing 10 % FBS under normoxia. De novo lipid synthesis was greater using glucose carbons than glutamine carbons under normoxia. The reverse was observed for most lipids under hypoxia suggesting an attenuation of glucose entering the TCA cycle. Lactate was produced largely from glucose carbons with minimal lactate derived from glutamine under either normoxia or hypoxia. Accumulation of triacylglycerols (TAG), containing mostly saturated and mono-unsaturated fatty acids, was observed under hypoxia using carbons from either glucose or glutamine. The data show that the incorporation of labeled glucose and glutamine into most synthesized lipids was dependent on the type of growth environment, and that the VM-M3 glioblastoma cells could acquire lipids, especially cholesterol, from the external environment for growth and proliferation. PMID:26537505

  19. Serum IgE reactivity profiling in an asthma affected cohort.

    Directory of Open Access Journals (Sweden)

    Tania Dottorini

    Full Text Available BACKGROUND: Epidemiological evidence indicates that atopic asthma correlates with high serum IgE levels though the contribution of allergen specific IgE to the pathogenesis and the severity of the disease is still unclear. METHODS: We developed a microarray immunoassay containing 103 allergens to study the IgE reactivity profiles of 485 asthmatic and 342 non-asthmatic individuals belonging to families whose members have a documented history of asthma and atopy. We employed k-means clustering, to investigate whether a particular IgE reactivity profile correlated with asthma and other atopic conditions such as rhinitis, conjunctivitis and eczema. RESULTS: Both case-control and parent-to-siblings analyses demonstrated that while the presence of specific IgE against individual allergens correlated poorly with pathological conditions, particular reactivity profiles were significantly associated with asthma (p<10E-09. An artificial neural network (ANN-based algorithm, calibrated with the profile reactivity data, correctly classified as asthmatic or non-asthmatic 78% of the individual examined. Multivariate statistical analysis demonstrated that the familiar relationships of the study population did not affect the observed correlations. CONCLUSIONS: These findings indicate that asthma is a higher-order phenomenon related to patterns of IgE reactivity rather than to single antibody reactions. This notion sheds new light on the pathogenesis of the disease and can be readily employed to distinguish asthmatic and non-asthmatic individuals on the basis of their serum reactivity profile.

  20. Triglyceride levels are closely associated with mild declines in estimated glomerular filtration rates in middle-aged and elderly Chinese with normal serum lipid levels.

    Directory of Open Access Journals (Sweden)

    Xinguo Hou

    Full Text Available OBJECTIVE: To investigate the relationship between lipid profiles [including total cholesterol (TC, triglyceride (TG, low-density lipoprotein cholesterol (LDL-C and high-density lipoprotein cholesterol (HDL-C] and a mild decline in the estimated glomerular filtration rate (eGFR in subjects with normal serum lipid levels. DESIGN AND METHODS: In this study, we included 2647 participants who were ≥ 40 years old and had normal serum lipid levels. The Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI equation was used to estimate the GFR. A mildly reduced eGFR was defined as 60-90 mL/min/1.73 m(2. First, multiple linear regression analysis was used to estimate the association of lipid profiles with the eGFR. Then, the levels of each lipid component were divided into four groups, using the 25th, 50th and 75th percentiles as cut-off points. Finally, multiple logistic regression analysis was used to investigate the association of different lipid components with the risk of mildly reduced eGFR. RESULTS: In the group with a mildly reduced eGFR, TG and LDL-C levels were significantly increased, but HDL-C levels were significantly decreased. After adjusting for age, gender, body mass index (BMI, systolic blood pressure (SBP, glycated hemoglobin (HbA1c, smoking and drinking, only TC and TG were independently related to the eGFR. Additionally, only TG showed a linear relationship with an increased risk of a mildly reduced eGFR, with the highest quartile group (TG: 108-150 mg/dl [1.22-1.70 mmol/L] having a significantly increased risk after adjusting for the above factors. CONCLUSIONS: Triglyceride levels are closely associated with a mildly reduced eGFR in subjects with normal serum lipid levels. Dyslipidemia with lower TG levels could be used as new diagnostic criteria for subjects with mildly reduced renal function.

  1. Trends in the Levels of Serum Lipids and Lipoproteins and the Prevalence of Dyslipidemia in Adults with Newly Diagnosed Type 2 Diabetes in the Southwest Chinese Han Population during 2003–2012

    OpenAIRE

    Jing Tian; Hewen Chen; Fang Jia; Gangyi Yang; Shengbing Li; Ke Li; Lili Zhang; Jinlin Wu; Dongfang Liu

    2015-01-01

    Objective. To determine the trends of serum lipid levels and dyslipidemia in adults newly diagnosed with type 2 diabetes mellitus during 2003–2012 in Southwest China. Methods. Serum lipid measurements of 994 adults were obtained from 5 independent, cross-sectional studies (2003-2004, 2005-2006, 2007-2008, 2009-2010, and 2011-2012). The main outcome measures were mean serum total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and triglyceride levels; bo...

  2. Serum lipids and apolipoprotein B values, blood pressure and pulse rate in anorexia nervosa.

    Science.gov (United States)

    Sánchez-Muniz, F J; Marcos, A; Varela, P

    1991-01-01

    Some risk factors associated with coronary heart disease (CHD) were evaluated in patients with different types of anorexia nervosa (AN). Anthropometric parameters, serum cholesterol, triglycerides and apoprotein (apo) B values, blood pressure and pulse rate were tested in 29 young female patients and 16 controls. Cholesterol, triglycerides and apo B were higher at the acute period of the illness (AN1), whereas at the chronic period the values of these parameters tended to normalize. Triglycerides were higher in patients who binge ate (bulimarexia). Systolic blood pressure decreased in all types of AN, while diastolic blood pressure decreased only in AN1; pulse rate was not altered. According to cholesterol and apo B values, AN patients may be at risk of CHD if they remain at low body weight. PMID:1855497

  3. Assessment of confounding factors affecting the tumor markers SMRP, CA125, and CYFRA21-1 in serum

    OpenAIRE

    Daniel Gilbert Weber; Georg Johnen; Dirk Taeger; Anne Weber; Isabelle Mercedes Gross; Beate Pesch; Thomas Kraus; Thomas Brüning; Monika Gube

    2010-01-01

    The purpose of this analysis was to evaluate if serum levels of potential tumor markers for the diagnosis of malignant mesothelioma and lung cancer are affected by confounding factors in a surveillance cohort of workers formerly exposed to asbestos. SMRP, CA125, and CYFRA21-1 concentrations were determined in about 1,700 serum samples from 627 workers formerly exposed to asbestos. The impact of factors that could modify the concentrations of the tumor markers was examined with linear mixed mo...

  4. Nonbilayer lipids affect peripheral and integral membrane proteins via changes in the lateral pressure profile.

    Science.gov (United States)

    van den Brink-van der Laan, Els; Killian, J Antoinette; de Kruijff, Ben

    2004-11-01

    Nonbilayer lipids can be defined as cone-shaped lipids with a preference for nonbilayer structures with a negative curvature, such as the hexagonal phase. All membranes contain these lipids in large amounts. Yet, the lipids in biological membranes are organized in a bilayer. This leads to the question: what is the physiological role of nonbilayer lipids? Different models are discussed in this review, with a focus on the lateral pressure profile within the membrane. Based on this lateral pressure model, predictions can be made for the effect of nonbilayer lipids on peripheral and integral membrane proteins. Recent data on the catalytic domain of Leader Peptidase and the potassium channel KcsA are discussed in relation to these predictions and in relation to the different models on the function of nonbilayer lipids. The data suggest a general mechanism for the interaction between nonbilayer lipids and membrane proteins via the membrane lateral pressure. PMID:15519321

  5. The effects of anaerobic training in serum lipids and arachidonic acid metabolites

    Directory of Open Access Journals (Sweden)

    GEORGIOS KIPREOS

    2010-01-01

    Full Text Available Coronary arteries are subjected daily in high shear stress and manifest atherosclerosis very early in life in comparison to other arteries in the human body. Some factors that are implicated in the evolution and progress of this process are the concentration of lipids and arachidonic acid metabolites, such prostacyclin and thromboxane. It has been reported that those who participate in aerobic activities such as walking, cycling, jogging or brisk walking might have normal values of the mentioned chemical substances. On the other hand, it is reported that the effects of anaerobic and strength activities has negative effects on the vascular endothelium, which is essential for the maintenance of hemostatic balance and the local regulation of vascular tone.Therefore, even although extensive research has been conducted in this field, there are crucial gaps in our knowledge. Consequently, the purpose of this brief review is to describe what is known about the effects of anaerobic activities in which the competitive athletes have participated on the following blood parameters: Total cholesterol, triglycerides, high density lipoprotein cholesterol (HDL - C, low density lipoproteins cholesterol (LDL - C, prostacyclin & thromboxane.

  6. Protective effect of irradiated licorice roots (Glycyrrhiza Glabra) on ethanol-induced serum lipid changes and liver Injury In rats

    International Nuclear Information System (INIS)

    Water extract of licorice roots irradiated with gamma rays at dose level of 20 KGy has been evaluated for hepato protective and hypolipidaemic effects against chronic ethanol mediated toxicity in rats.Ethanol administration to rats (7.9 g/kg/day) for 45 days resulted in significant elevated levels of serum total bilirubin, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total cholesterol (TC), triglycerides (TG), low density lipoprotein-cholesterol (LDL-C), thiobarbituric acid reactive substances (TBARS). Meanwhile, significant reductions in high density lipoprotein-cholesterol (HDL-C), total protein, albumin and glucose were noticed in comparison to those of control group, whereas, serum Na+ , K+ and globulin were kept unchanged. Co administration of raw and irradiated licorice roots (3 g/l in drinking water) with the daily dose of ethanol caused significant improvement in all the above mentioned parameters except serum glucose level. On the other hand, results showed that licorice roots water extract induced significant decrease in K+ level with increased Na+ level. There was non-significant difference between non-irradiated and irradiated licorice. Thus, it could be concluded that irradiated licorice roots has not affected its physiological functions. Moreover, supplementation with licorice roots water extract can offer protection against free radical mediated oxidative stress in hepatotoxicity

  7. Erythrocyte-bound apolipoprotein B in relation to atherosclerosis, serum lipids and ABO blood group.

    Directory of Open Access Journals (Sweden)

    Boudewijn Klop

    Full Text Available INTRODUCTION: Erythrocytes carry apolipoprotein B on their membrane, but the determining factors of erythrocyte-bound apolipoprotein B (ery-apoB are unknown. We aimed to explore the determinants of ery-apoB to gain more insight into potential mechanisms. METHODS: Subjects with and without CVD were included (N = 398. Ery-apoB was measured on fresh whole blood samples using flow cytometry. Subjects with ery-apoB levels ≤ 0.20 a.u. were considered deficient. Carotid intima media thickness (CIMT was determined as a measure of (subclinical atherosclerosis. RESULTS: Mean ery-apoB value was 23.2% lower in subjects with increased CIMT (0.80 ± 0.09 mm, N = 140 compared to subjects with a normal CIMT (0.57 ± 0.08 mm, N = 258 (P = 0.007, adjusted P<0.001. CIMT and ery-apoB were inversely correlated (Spearman's r: -0.116, P = 0.021. A total of 55 subjects (13.6% were considered ery-apoB deficient, which was associated with a medical history of CVD (OR: 1.86, 95% CI 1.04-3.33; adjusted OR: 1.55; 95% CI 0.85-2.82. Discontinuation of statins in 54 subjects did not influence ery-apoB values despite a 58.4% increase in serum apolipoprotein B. Subjects with blood group O had significantly higher ery-apoB values (1.56 ± 0.94 a.u. when compared to subjects with blood group A (0.89 ± 1.15 a.u, blood group B (0.73 ± 0.1.12 a.u. or blood group AB (0.69 ± 0.69 a.u. (P-ANOVA = 0.002. CONCLUSION: Absence or very low values of ery-apoB are associated with clinical and subclinical atherosclerosis. While serum apolipoprotein B is not associated with ery-apoB, the ABO blood group seems to be a significant determinant.

  8. Study the Effects of Ramadan Fasting on the Serum Glucose and Lipid Profile among Healthy Jordanian Students

    Directory of Open Access Journals (Sweden)

    Kama M.S. Mansi

    2007-01-01

    Full Text Available Ramadan is the holiest month in the Islamic calendar and Muslims fast during this month. We designed this study to evaluate the effect of Ramadan fasting on plasma lipids profile and serum glucose among healthy Jordanian students. Ramadan fasting entails major changes in sleep pattern, physical activity and eating habits, which may cause changes in metabolism both in health and disease. This cohort study was performed during Ramadan in October 2006 (Islamic year 1427. The subjects were 70 healthy Jordanian students (male and female from three Jordanian universities who fasted during Ramadan. Their mean age was 21 ±1.6 years. We evaluated some anthropometric parameters as body weight (Kg, pulse rate (per minute and systolic and diastolic blood pressure (mmHg. All parameters at 4 week of Ramadan were significantly lower than pre-Ramadan values, 2 weeks after Ramadan fasting, body weight and other parameters had a trend to recoup to pre-Ramadan status; however, they were still lower than the pre-Ramadan values. We evaluated blood glucose, triglycerides (TG, cholesterol, low-density lipoprotein LDL, high-density lipoprotein (HDL, and Triglycerides at 1 day before, at week 1, 2, 4 of the Ramadan month and after tow weeks after the end of Ramadan. It was found that high-density lipoprotein (HDL cholesterol increased significantly during Ramadan, which indicated positive association with pulse rate and fat intake and negative association with systolic blood pressure and weight. . The LDLc was significantly reduced at the end of fasting. A reduction in the average TC value was observed at the end of fasting but the difference was not statistically and there no significant rise in the TG and blood sugar values at the end of fasting. This study indicated that Ramadan fasting led to a decrease weight, LDLc significantly, and significant not statistically reduction in the average TC value, and significantly increasing in HDLc was noted during Ramadan

  9. Osmotic and curvature stress affect PEG-induced fusion of lipid vesicles but not mixing of their lipids.

    OpenAIRE

    Malinin, Vladimir S.; Frederik, Peter; Lentz, Barry R

    2002-01-01

    Poly (ethylene glycol) (PEG) in the external environment of membrane vesicles creates osmotic imbalance that leads to mechanical stress in membranes and may induce local membrane curvature. To determine the relative importance of membrane stress and curvature in promoting fusion, we monitored contents mixing (CM) and lipid mixing (LM) between different sized vesicles under a variety of osmotic conditions. CM between highly curved vesicles (SUV, 26 nm diameter) was up to 10 times greater than ...

  10. Effects of Red Palm Oil on Serum Lipids and Plasma Carotenoids Level in Chinese Male Adults

    Institute of Scientific and Technical Information of China (English)

    JIAN ZHANG; CHUN-RONG WANG; AN-NA XUE; KE-YOU GE

    2003-01-01

    Objective Effects of red palm oil on major plasma carotenoids, tocopherol, retinol and serumlipids were evaluated when used in Chinese diet. Methods Red palm oil group (RPO) composed of 20 male subjects(aged 18-32) and soybean oil group (SBO) composed of 22 male subjects (aged18-32). Dietary fat provided about 28% of total calories, and the test oil accounted for about 60% of total dietary fat. In the 3 weeks of pretest period, diets were prepared with soybean oil, and then in the next 6 weeks subjects in each group consumed the diet prepared by test oil. Results Plasmaα-carotene, β-carotene and lycopene concentration of RPO group significantly increased at the time of interim (21 days) and of the end (42 days) (P<0.05), and α-tocopherol concentration significantly increased at the time of the end (42 days) in this study. Though Chinese plasma retinol level was relatively low when compared with that of Westerners, red palm oil diet showed no significant effect on adult Chinese plasma retinol level. Serum concentration of total cholesterol, triglyceride, high density lipoprotein cholesterol, apolipoprotein AI and apolipoprotein B of all subjects showed no significant changes in RPO group during the study. Conclusions The data in our study suggest that red palm oil is a good source of carotenoids and vitamin E when used in Chinese diet preparation, and it can significantly increase plasma concentration of α-carotene, β-carotene, lycopene andα-tocopherol.

  11. FRUCTOOLIGOSACCHARIDE (FOS SUPPLEMENTATION IN TYPE 2 DIABETIC ADULTS IMPROVES SYSTOLIC BLOOD PRESSURE, SERUM LIPID, AND GUT MICROBIOTA

    Directory of Open Access Journals (Sweden)

    Mahendra A

    2013-12-01

    Full Text Available Fructooligosaccharide (FOS is a prebiotic, becoming apparent for its therapeutic role for diseased conditions like CHD, hypertension, diabetes and obesity. CHD and hypertension are one of the most prevalent NCD’s encountered in diabetic adults. Present study aims to assess the effect of FOS supplementation in type 2 diabetic adults on their lipemic, biophysical parameters and gut microflora parameters. A cross-sectional study was designed with 65 adult type 2 diabetics enrolled from Health clinic of The M.S. University of Baroda, Gujarat. All the subjects were randomly divided into two groups control and experimental. The experimental group was given 10 g of fructooligosaccharide and compared with the controls for lipemic parameters, hypertension and fecal counts in terms of lactic acid bacteria, bifidobacteria and enteric pathogen. Eight week supplementation of FOS resulted in an appreciable reduction in serum TC, TG and LDL levels by 10%, 4.9% and 7.8% respectively. A significant reduction (p<0.05 in systolic blood pressure was also observed as a result of FOS supplementation in the experimental group. A decline was seen in TC/HDL, LDL/HDL and non-HDL by 10.4%, 7.6% and 6.6% respectively (p<0.05, p<0.001. Gut microbiota values exhibited a significant increment in fecal log10 counts of Lactic acid bacteria and bifidobacteria by 9.3% and 10.9% respectively (p<0.001 while a significant reduction by 4.8% (p<0.001 was observed for Enteric pathogen. These outcomes revealed an efficacy of FOS in reducing serum lipid parameters, systolic BP and improving the gut microbiota in type 2 diabetic adults

  12. Effect of the Medicinal Mushroom, Grifola gargal (Agaricomycetes), on Bone Turnover Markers and Serum Lipids in Middle-Aged and Elderly Japanese Women.

    Science.gov (United States)

    Harada, Etsuko; Morizono, Toshihiro; Sumiya, Toshimitsu; Kawagishi, Hirokazu

    2016-01-01

    A clinical study was performed to examine the effect of the edible mushroom, Grifola gargal, on bone turnover markers and serum lipids in middle-aged and elderly Japanese women. Postmenopausal women aged 51-73 years (mean age, 61 years) received daily oral administration of 5 g G. gargal fruiting bodies (hot air-dried and powdered; G. gargal powder [GGP]). Serum levels of bone alkaline phosphatase (BAP) and lipids and urinary deoxypyridinoline (DPD) levels were measured before and 2 weeks after the start of GGP treatment. As a result, urinary DPD bone resorption marker levels in women treated with GGP decreased significantly. Serum levels of the BAP bone formation marker also tended to increase, but the difference was not significant. By contrast, the atherogenic index decreased and the high-density lipoprotein (HDL) ratio increased significantly. However, there were no statistically significant differences in serum lipids of total cholesterol, HDL cholesterol, and low-density lipoprotein cholesterol. In addition, this study demonstrated for the first time that G. gargal is safe for human consumption. PMID:27279439

  13. Imbalanced Hemolymph Lipid Levels Affect Feeding Motivation in the Two-Spotted Cricket, Gryllus bimaculatus

    Science.gov (United States)

    Konuma, Takahiro; Tsukamoto, Yusuke; Nagasawa, Hiromichi; Nagata, Shinji

    2016-01-01

    Insect feeding behavior is regulated by many intrinsic factors, including hemolymph nutrient levels. Adipokinetic hormone (AKH) is a peptide factor that modulates hemolymph nutrient levels and regulates the nutritional state of insects by triggering the transfer of lipids into the hemolymph. We recently demonstrated that RNA interference (RNAi)-mediated knockdown of the AKH receptor (AKHR) reduces hemolymph lipid levels, causing an increase in the feeding frequency of the two-spotted cricket, Gryllus bimaculatus. This result indicated that reduced hemolymph lipid levels might motivate crickets to feed. In the present study, to elucidate whether hemolymph lipid levels contribute to insect feeding behavior, we attempted to manipulate hemolymph lipid levels via the lipophorin (Lp)-mediated lipid transferring system in G. bimaculatus. Of the constituent proteins in Lp, we focused on apolipophorin-III (GrybiApoLp-III) because of its possible role in facilitating lipid mobilization. First, we used RNAi to reduce the expression of GrybiApoLp-III. RNAi-mediated knockdown of GrybiApoLp-III had little effect on basal hemolymph lipid levels and the amount of food intake. In addition, hemolymph lipid levels remained static even after injecting AKH into GrybiApoLp-IIIRNAi crickets. These observations indicated that ApoLp-III does not maintain basal hemolymph lipid levels in crickets fed ad libitum, but is necessary for mobilizing lipid transfer into the hemolymph following AKH stimulation. Second, Lp (containing lipids) was injected into the hemolymph to induce a temporary increase in hemolymph lipid levels. Consequently, the initiation of feeding was delayed in a dose-dependent manner, indicating that increased hemolymph lipid levels reduced the motivation to feed. Taken together, these data validate the importance of basal hemolymph lipid levels in the control of energy homeostasis and for regulating feeding behavior in crickets. PMID:27144650

  14. Imbalanced Hemolymph Lipid Levels Affect Feeding Motivation in the Two-Spotted Cricket, Gryllus bimaculatus.

    Directory of Open Access Journals (Sweden)

    Takahiro Konuma

    Full Text Available Insect feeding behavior is regulated by many intrinsic factors, including hemolymph nutrient levels. Adipokinetic hormone (AKH is a peptide factor that modulates hemolymph nutrient levels and regulates the nutritional state of insects by triggering the transfer of lipids into the hemolymph. We recently demonstrated that RNA interference (RNAi-mediated knockdown of the AKH receptor (AKHR reduces hemolymph lipid levels, causing an increase in the feeding frequency of the two-spotted cricket, Gryllus bimaculatus. This result indicated that reduced hemolymph lipid levels might motivate crickets to feed. In the present study, to elucidate whether hemolymph lipid levels contribute to insect feeding behavior, we attempted to manipulate hemolymph lipid levels via the lipophorin (Lp-mediated lipid transferring system in G. bimaculatus. Of the constituent proteins in Lp, we focused on apolipophorin-III (GrybiApoLp-III because of its possible role in facilitating lipid mobilization. First, we used RNAi to reduce the expression of GrybiApoLp-III. RNAi-mediated knockdown of GrybiApoLp-III had little effect on basal hemolymph lipid levels and the amount of food intake. In addition, hemolymph lipid levels remained static even after injecting AKH into GrybiApoLp-IIIRNAi crickets. These observations indicated that ApoLp-III does not maintain basal hemolymph lipid levels in crickets fed ad libitum, but is necessary for mobilizing lipid transfer into the hemolymph following AKH stimulation. Second, Lp (containing lipids was injected into the hemolymph to induce a temporary increase in hemolymph lipid levels. Consequently, the initiation of feeding was delayed in a dose-dependent manner, indicating that increased hemolymph lipid levels reduced the motivation to feed. Taken together, these data validate the importance of basal hemolymph lipid levels in the control of energy homeostasis and for regulating feeding behavior in crickets.

  15. Imbalanced Hemolymph Lipid Levels Affect Feeding Motivation in the Two-Spotted Cricket, Gryllus bimaculatus.

    Science.gov (United States)

    Konuma, Takahiro; Tsukamoto, Yusuke; Nagasawa, Hiromichi; Nagata, Shinji

    2016-01-01

    Insect feeding behavior is regulated by many intrinsic factors, including hemolymph nutrient levels. Adipokinetic hormone (AKH) is a peptide factor that modulates hemolymph nutrient levels and regulates the nutritional state of insects by triggering the transfer of lipids into the hemolymph. We recently demonstrated that RNA interference (RNAi)-mediated knockdown of the AKH receptor (AKHR) reduces hemolymph lipid levels, causing an increase in the feeding frequency of the two-spotted cricket, Gryllus bimaculatus. This result indicated that reduced hemolymph lipid levels might motivate crickets to feed. In the present study, to elucidate whether hemolymph lipid levels contribute to insect feeding behavior, we attempted to manipulate hemolymph lipid levels via the lipophorin (Lp)-mediated lipid transferring system in G. bimaculatus. Of the constituent proteins in Lp, we focused on apolipophorin-III (GrybiApoLp-III) because of its possible role in facilitating lipid mobilization. First, we used RNAi to reduce the expression of GrybiApoLp-III. RNAi-mediated knockdown of GrybiApoLp-III had little effect on basal hemolymph lipid levels and the amount of food intake. In addition, hemolymph lipid levels remained static even after injecting AKH into GrybiApoLp-IIIRNAi crickets. These observations indicated that ApoLp-III does not maintain basal hemolymph lipid levels in crickets fed ad libitum, but is necessary for mobilizing lipid transfer into the hemolymph following AKH stimulation. Second, Lp (containing lipids) was injected into the hemolymph to induce a temporary increase in hemolymph lipid levels. Consequently, the initiation of feeding was delayed in a dose-dependent manner, indicating that increased hemolymph lipid levels reduced the motivation to feed. Taken together, these data validate the importance of basal hemolymph lipid levels in the control of energy homeostasis and for regulating feeding behavior in crickets. PMID:27144650

  16. Bifidogenic effect of grain larvae extract on serum lipid, glucose and intestinal microflora in rats

    Indian Academy of Sciences (India)

    Sang-Oh Park; Byung-Sung Park

    2015-09-01

    The main objective of this study was to investigate whether orally administered Korean grain larvae ethanol extract (GLE) had a bifidogenic effect in normal rats. Male Sprague–Dawley rats were divided into a negative control group (CO) and GLE orally administered (5.0, 7.0 and 9.0 mg/100 g body weight) groups. Thymus and spleen weights dosedependently increased by 128.58% and 128.58%, respectively, but abdominal fat decreased by 19.18% after GLE administration compared with that in the CO group (<0.05). Serum triglycerides, total cholesterol, low-density lipoprotein cholesterol, and glucose decreased by 30.26%, 7.33%, 27.20%, and 6.96%, respectively, whereas highdensity lipoprotein cholesterol increased by 129.93% in the GLE groups compared with those in the CO group (<0.05). IgG, IgM, IgA in the GLE groups increased 203.68%, 181.41%, and 238.25%, respectively, compared to that in the CO group (<0.05). Bifidobacteria and Lactobacillus increased by 115.74% and 144.28%, whereas Bacteroides, Clostridium, Escherichia, and Streptococcus decreased by 17.37%, 17.46%, 21.25%, and 19.16%, respectively, in the GLE groups compared with those in the CO group (<0.05). Total organic acids, acetic acid, and propionic acid increased by 151.40%, 188.09%, and 150.17%, whereas butyric acid and valeric acid decreased by 40.65% and 49.24%, respectively, in the GLE groups as compared with those in the CO group (<0.05). These results suggest that Korean GLE improves the bifidogenic effect by increasing cecal organic acids and modulating gut microflora via a selective increase in Bifidobacterium in normal rats.

  17. Comparing the effect of lovastatin and exercise on serum lipid profile in hyperlipidaemic patients

    Directory of Open Access Journals (Sweden)

    Taghadosi M

    2013-01-01

    Full Text Available Background: Hyperlipidaemic is a risk factor of cardiovascular diseases. Recently, statins, life style modification and diet have been used to treat hyperlipidaemia. Aim: The present study was performed to evaluate the effect of lovastatin and exercise on hyperlipidaemic patients. Methods: This double-blind randomized clinical trial was performed on 80 patients over 30 years old, whose lipid profile was HDL ≤35 mg/dl, LDL ≥160 mg/dl, triglyceride ≥200 mg/dl, and cholesterol ≥200. At first, 96 hyperlipidemic patients were included in this study, 7 out of them were excluded because of HTN (hypertension and HLP (hyperlipidaemia and 9 due to diabetes. The patients were randomly divided into two groups: the first group took lovastatin 40 mg daily, the second group was instructed to walk 35 minutes a day, 3 days a week. After 6 weeks trial, data were analyzed using t-test, Wilcoxon signed sample test, Kolomogorou-Smirnov test, Mann-Whitney test, and Leveus test. Results: The average age was 55.18±9.9. 59 out of 80 (73.7% were men and 21 were women (26.3%. The average age of group 1(with lovastin was 55.12±8.4 years and in group 2 (exercise was 55.35±9.2. The average decrease in TG level in lovastatin treated (I and subjected to exercise (II groups was 21.4 mg/dl and 20.5 mg/dl (P<0.2 respectively. Cholesterol decrease in two groups was 41.3±44.3 mg/dl and 1.57±41.8 mg/dl (P<0.001 respectively. The average decrease in LDL level in I and II groups was 35.82±34.1and 8.55±28.1 (P<0.001.whereas, an average increase was shown in HCL level: 5.45±14.4mg/dl and 0.4±12.3 mg/dl (P-value = 0.09 respectively. Conclusion: The results showed that exercise could reduce LDL, but lovastatin can reduce total cholesterol, TG and can increase HDL. Therefore in the management of hyperlipidaemia, exercise should be combined with lovastatin therapy to achieve a better outcome.

  18. Detection of serum iron and zinc levels in patients with type 2 diabetes mellitus and their relationship with glucose and lipid metabolism

    Institute of Scientific and Technical Information of China (English)

    Xiao-Yong Tang; Wan-Fen Zhong

    2016-01-01

    Objective:To study the blood iron and zinc levels in patients with type 2 diabetes mellitus and their relationship with glucose and lipid metabolism.Methods:Patients with type 2 diabetes mellitus were enrolled in T2DM group and healthy people were enrolled in control group. T2DM patients were divided into low iron and zinc group and high iron and zinc group with the median of serum iron and zinc contents as the standard. Serum trace elements contents and glucose and lipid metabolism indicators were detected.Results:T2DM patients’ blood iron contents were significantly higher than those of the control group while the zinc contents were significantly lower than those of the control group. Copper, calcium and magnesium contents had no significant difference between two groups of patients. HbA1c%, glucagon and HOMA-IR as well as apoB, apoE, ApoM, leptin and vaspin contents in high iron group were higher than those in low iron group while serum insulin, GLP-1, GIP, ApoA1, APN and HOMA-β levels were lower than those in low iron group. HbA1c%, glucagon, HOMA-IR, apoB, apoE, ApoM, leptin and vaspin contents in high zinc group were lower than those in low zinc group while serum insulin, GLP-1, GIP, ApoA1, APN and HOMA-β levels were higher than those in low zinc group.Conclusions: the serum iron level was abnormally high and the zinc content was abnormally low in patients with type 2 diabetes mellitus. Serum iron and zinc levels were closely related to the glucose and lipid metabolism.

  19. Long-term effects of consuming foods containing psyllium seed husk on serum lipids in subjects with hypercholesterolemia.

    Science.gov (United States)

    Davidson, M H; Maki, K C; Kong, J C; Dugan, L D; Torri, S A; Hall, H A; Drennan, K B; Anderson, S M; Fulgoni, V L; Saldanha, L G; Olson, B H

    1998-03-01

    The effects of consuming foods containing 0 (control), 3.4, 6.8, or 10.2 g psyllium seed husk (PSH)/d for 24 wk on the serum lipid profile were assessed in this randomized, double-blind controlled study. Men and women (n = 286) with LDL-cholesterol concentrations between 3.36 and 5.68 mmol/L (130 and 220 mg/dL) were randomly assigned to one of four treatment groups after following a low-fat diet for > or = 8 wk. At week 24, LDL cholesterol was 3% above baseline in the control group. In the group consuming 10.2 g PSH/d, LDL cholesterol remained below baseline during treatment, with a value 5.3% below that of the control group at week 24 (P < 0.05 compared with the control group). No significant differences were observed in HDL cholesterol or triacylglycerol. Although modest, the effect of 10.2 g PSH/d on LDL cholesterol (relative to the control) persisted throughout the 24-wk treatment period, indicating potential for long-term benefit. PMID:9497178

  20. Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/α-albumin.

    Science.gov (United States)

    Mihara, Emiko; Hirai, Hidenori; Yamamoto, Hideki; Tamura-Kawakami, Keiko; Matano, Mami; Kikuchi, Akira; Sato, Toshiro; Takagi, Junichi

    2016-01-01

    Wnt plays important role during development and in various diseases. Because Wnts are lipidated and highly hydrophobic, they can only be purified in the presence of detergents, limiting their use in various in vitro and in vivo assays. We purified N-terminally tagged recombinant Wnt3a secreted from cells and accidentally discovered that Wnt3a co-purified with a glycoprotein afamin derived from the bovine serum included in the media. Wnt3a forms a 1:1 complex with afamin, which remains soluble in aqueous buffer after isolation, and can induce signaling in various cellular systems including the intestical stem cell growth assay. By co-expressing with afamin, biologically active afamin-Wnt complex can be easily obtained in large quantity. As afamin can also solubilize Wnt5a, Wnt3, and many more Wnt subtypes, afamin complexation will open a way to put various Wnt ligands and their signaling mechanisms under a thorough biochemical scrutiny that had been difficult for years. PMID:26902720

  1. Effects of long-term moderate exercise and increase in number of daily steps on serum lipids in women: randomised controlled trial [ISRCTN21921919

    Directory of Open Access Journals (Sweden)

    Mirbod Seyed

    2002-01-01

    Full Text Available Abstract Background This study was designed to evaluate the effects of a 24-month period of moderate exercise on serum lipids in menopausal women. Methods The subjects (40–60 y were randomly divided into an exercise group (n = 14 and a control group (n = 13. The women in the exercise group were asked to participate in a 90-minute physical education class once a week and to record their daily steps as measured by a pedometer for 24 months. Results Mean of daily steps was significantly higher in the exercise group from about 6,800 to over 8,500 steps (P P Conclusions These results suggest that daily exercise as well as increasing the number of daily steps can improve the profile of serum lipids.

  2. The V227A polymorphism at the PPARA locus is associated with serum lipid concentrations and modulates the association between dietary polyunsaturated fatty acid intake and serum high density lipoprotein concentrations in Chinese women.

    Science.gov (United States)

    Chan, Edmund; Tan, Chuen Seng; Deurenberg-Yap, Mabel; Chia, Kee Seng; Chew, Suok Kai; Tai, E Shyong

    2006-08-01

    Peroxisome proliferators activated receptor alpha (PPARalpha) regulates the transcription of several proteins involved in human lipoprotein metabolism. We screened the PPARA locus for polymorphisms in 20 unrelated subjects from each of three ethnic groups (Chinese, Malays and Asian Indians). Only the V227A polymorphism was observed. We genotyped 4248 subjects (2899 Chinese, 761 Malay and 588 Asian Indians) and found allele frequencies for the A227 allele of 0.04 in Chinese, 0.006 in Malays and 0.003 in Asian Indians. We examined the associations between this polymorphism and serum lipid concentrations in Chinese. In women, but not in men, the presence of the A227 allele was associated with lower serum concentrations of total cholesterol [5.38mmol/l (95%CI: 5.22-5.54) versus 5.21mmol/l (95%CI: 4.99-5.43), p=0.047] and triglycerides [1.19mmol/l (95%CI: 1.10-1.28) versus 1.09mmol/l (95%CI: 0.98-1.21), p=0.048]. We also found that the V227A polymorphism modulates the association between dietary polyunsaturated fatty acid intake and serum high density lipoprotein concentration (p-value for interaction=0.049). Our findings implicate PPARalpha in the lipid lowering associated with diets high in PUFA and suggests that genetic variation at the PPARA locus may determine the lipid response to changes in PUFA intake. PMID:16288935

  3. Plasma membrane lipid-protein interactions affect signaling processes in sterol-biosynthesis mutants of Arabidopsis thaliana

    Directory of Open Access Journals (Sweden)

    Henrik eZauber

    2014-03-01

    Full Text Available The plasma membrane is an important organelle providing structure, signaling and transport as major biological functions. Being composed of lipids and proteins with different physicochemical properties, the biological functions of membranes depend on specific protein-protein and protein-lipid interactions. Interactions of proteins with their specific sterol and lipid environment were shown to be important factors for protein recruitment into sub-compartmental structures of the plasma membrane. System-wide implications of altered endogenous sterol levels for membrane functions in living cells were not studied in higher plant cells. In particular, little is known how alterations in membrane sterol composition affect protein and lipid organization and interaction within membranes. Here, we conducted a comparative analysis of the plasma membrane protein and lipid composition in Arabidopsis sterol-biosynthesis mutants smt1 and ugt80A2;B1. smt1 shows general alterations in sterol composition while ugt80A2;B1 is significantly impaired in sterol glycosylation. By systematically analyzing different cellular fractions and combining proteomic with lipidomic data we were able to reveal contrasting alterations in lipid-protein interactions in both mutants, with resulting differential changes in plasma membrane signaling status.

  4. Polyphenol-rich extract from blackcurrant pomace attenuates the intestinal tract and serum lipid changes induced by a high-fat diet in rabbits

    OpenAIRE

    Jurgoński, Adam; Juśkiewicz, Jerzy; Zduńczyk, Zenon; Matusevicius, Paulius; Kołodziejczyk, Krzysztof

    2014-01-01

    Purpose The consumption of a high level of dietary extract from blackcurrant pomace rich in polyphenols was hypothesised to exert beneficial effects on the serum lipid profile, the markers of insulin resistance and the antioxidant status of the host without negative changes in the intestinal tract. Methods This hypothesis was tested on 20 male New Zealand white rabbits randomly assigned to four groups of five individuals each. For 4 weeks, the animals were subjected to the following dietary t...

  5. Fatty acids in serum lipid fractions as indicators of fat intake in 5-year-old children in the STRIP project

    OpenAIRE

    Hagström, Hanna; Jokinen, Eero; Seppänen, Ritva; Salo, Pia; Viikari, Jorma; Rönnemaa, Tapani; Helenius, Hans; Simell, Olli

    1998-01-01

    The objective of the present study was to investigate the association between dietary fat composition and the proportion of fatty acids in serum lipid fractions as biomarkers of previous dietary fat quality in 5-year-old children. Using 4-consecutive-day food records, the food consumption of half (n=50) of a group of randomly selected children who are participating in a prospective randomized coronary risk factor intervention project (STRIP; n=1062 at recruitment at 7 months of age) was estim...

  6. Higher endogenous methionine in transgenic Arabidopsis seeds affects the composition of storage proteins and lipids.

    Science.gov (United States)

    Cohen, Hagai; Pajak, Agnieszka; Pandurangan, Sudhakar; Amir, Rachel; Marsolais, Frédéric

    2016-06-01

    Previous in vitro studies demonstrate that exogenous application of the sulfur-containing amino acid methionine into cultured soybean cotyledons and seedlings reduces the level of methionine-poor storage proteins and elevates those that are methionine-rich. However, the effect of higher endogenous methionine in seeds on the composition of storage products in vivo is not studied yet. We have recently produced transgenic Arabidopsis seeds having significantly higher levels of methionine. In the present work we used these seeds as a model system and profiled them for changes in the abundances of 12S-globulins and 2S-albumins, the two major groups of storage proteins, using 2D-gels and MALDI-MS detection. The findings suggest that higher methionine affects from a certain threshold the accumulation of several subunits of 12S-globulins and 2S-albumins, regardless of their methionine contents, resulting in higher total protein contents. The mRNA abundances of most of the genes encoding these proteins were either correlated or not correlated with the abundances of these proteins, implying that methionine may regulate storage proteins at both transcriptional and post-transcriptional levels. The elevations in total protein contents resulted in reduction of total lipids and altered the fatty acid composition. Altogether, the data provide new insights into the regulatory roles of elevated methionine levels on seed composition. PMID:26888094

  7. Effect of a phytogenic feed additive on performance, ovarian morphology, serum lipid parameters and egg sensory quality in laying hen.

    Science.gov (United States)

    Saki, Ali Asghar; Aliarabi, Hassan; Hosseini Siyar, Sayed Ali; Salari, Jalal; Hashemi, Mahdi

    2014-01-01

    This present study was conducted to evaluate the effects of dietary inclusion of 4, 8 and 12 g kg(-1) phytogenic feed additives mixture on performance, egg quality, ovary parameters, serum biochemical parameters and yolk trimethylamine level in laying hens. The results of experiment have shown that egg weight was increased by supplementation of 12 g kg(-1) feed additive whereas egg production, feed intake and feed conversion ratio (FCR) were not significantly affected. There were no significant differences in egg quality parameters by supplementation of phytogenic feed additive, whereas yolk trimethylamine level was decreased as the feed additive level increased. The sensory evaluation parameters did not differ significantly. No significant differences were found in serum cholesterol and triglyceride levels between the treatments but low- and high-density lipoprotein were significantly increased. Number of small follicles and ovary weight were significantly increased by supplementation of 12 g kg(-1) feed additive. Overall, dietary supplementation of polyherbal additive increased egg weigh, improved ovary characteristics and declined yolk trimethylamine level. PMID:25610580

  8. Effects of traditional Chinese medicines on serum lipid profiles and homocysteine in the ovariectomized rats.

    Science.gov (United States)

    Lee, Su-Chen; Chang, Shun-Jen; Tsai, Li-Yu

    2004-01-01

    To investigate the effects of the traditional Chinese medicines, kuei-lu-erh-hsien-chiao and chia-wei-hsiao-yao-san, on the cardiovascular systems of mimic menopausal rats, five groups were formed: group 1 (the control group) was given a sham operation and received distilled water, while groups 2, 3, 4 and 5 were ovariectomized and received distilled water, kuei-lu-erh-hsien-chiao, chia-wei-hsiao-yao-san and 17-beta-estradiol, respectively, for4 months. Our results demonstrated that the mean differences of the estrogen levels in groups 3 or 5 were significantly higher than those of group 2. These data suggest that there might be some estrogen-like substances in kuei-lu-erh-hsien-chiao. However, the function of these estrogen-like substances was unknown. The mean differences of the triglyceride (TG) levels, high-density lipoprotein cholesterol (HDL-C) levels, low-density lipoprotein cholesterol (LDL-C) levels, and the ratios of TC/HDL-C and LDL-C/HDL-C in groups 1, 3, 4 and 5 were not significantly different from those in group 2. The mean differences of the total cholesterol (TC) levels in group 5 were significantly higher than those in group 2 (p chia-wei-hsiao-yao-san declines the Hcy levels in OVX rats and does not affect the TC levels in these animals. In conclusion, our results indicate that chia-wei-hsiao-yao-san shows a more profound effect than 17-beta-estradiol in the prevention of atherosclerosis in these OVX rats. PMID:15481644

  9. Lipid hydrolysis products affect the composition of infant gut microbial communities in vitro

    DEFF Research Database (Denmark)

    Bennike, Rikke Mette Guldhammer; Bahl, Martin Iain; Vigsnæs, Louise Kristine;

    2015-01-01

    14 : 0 and MAG 12 : 0) or long-chained NEFA (LC-NEFA; 16 : 0 to 18 : 1 and MAG 16 : 0) with and without sphingosine, representing lipid hydrolysis products characteristic for intestinal hydrolysis of breast milk lipids. Ion Torrent sequencing of the bacterial 16S ribosomal RNA gene revealed that the...

  10. Association of MYLIP rs3757354 SNP and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations

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    Yan Ting-Ting

    2012-10-01

    Full Text Available Abstract Background The association of rs3757354 single nucleotide polymorphism (SNP in the E3 ubiquitin ligase myosin regulatory light chain-interacting protein (MYLIP, also known as IDOL gene and serum lipid levels is not well known in the general population. The present study aimed to detect the association of rs3757354 SNP and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. Method A total of 627 subjects of Bai Ku Yao minority and 614 participants of Han nationality were randomly selected from our stratified randomized cluster samples. Genotyping of the rs3757354 SNP was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing. Results The levels of serum total cholesterol (TC, high-density lipoprotein cholesterol (HDL-C, low-density lipoprotein cholesterol (LDL-C, apolipoprotein (Apo AI and ApoB were lower in Bai Ku Yao than in Han (P P P 0.05; respectively. There were no significant differences in the genotypic and allelic frequencies between males and females in both ethnic groups. The levels of HDL-C in Bai Ku Yao were different among the genotypes (P P P P Conclusions The present study suggests that the MYLIP rs3757354 SNP is associated with serum TC, HDL-C and ApoAI levels in the Bai Ku Yao and Han populations. But the association is different between the two ethnic groups.

  11. Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

    Directory of Open Access Journals (Sweden)

    Cao Xiao-Li

    2010-10-01

    Full Text Available Abstract Background The association of methylenetetrahydrofolate reductase (MTHFR gene polymorphism and serum lipid profiles is still controversial in diverse ethnics. Bai Ku Yao is an isolated subgroup of the Yao minority in China. The aim of the present study was to eveluate the association of MTHFR C677T polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. Methods A total of 780 subjects of Bai Ku Yao and 686 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples. Genotyping of the MTHFR C677T was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing. Results The levels of serum total cholesterol (TC, high-density lipoprotein cholesterol (HDL-C, low-density lipoprotein cholesterol (LDL-C, apolipoprotein (Apo AI and ApoB were lower in Bai Ku Yao than in Han (P P P P P P P Conclusions The differences in serum TC, TG, LDL-C and ApoB levels between the two ethnic groups might partly result from different genotypic and allelic frequencies of the MTHFR C677T or different MTHFR gene-enviromental interactions.

  12. Lipid emulsions differentially affect LPS-induced acute monocytes inflammation: in vitro effects on membrane remodeling and cell viability.

    Science.gov (United States)

    Boisramé-Helms, Julie; Delabranche, Xavier; Klymchenko, Andrey; Drai, Jocelyne; Blond, Emilie; Zobairi, Fatiha; Mely, Yves; Hasselmann, Michel; Toti, Florence; Meziani, Ferhat

    2014-11-01

    The aim of this study was to assess how lipid emulsions for parenteral nutrition affect lipopolysaccharide (LPS)-induced acute monocyte inflammation in vitro. An 18 h long LPS induced human monocyte leukemia cell stimulation was performed and the cell-growth medium was supplemented with three different industrial lipid emulsions: Intralipid(®), containing long-chain triglycerides (LCT--soybean oil); Medialipid(®), containing LCT (soybean oil) and medium-chain triglycerides (MCT--coconut oil); and SMOFlipid(®), containing LCT, MCT, omega-9 and -3 (soybean, coconut, olive and fish oils). Cell viability and apoptosis were assessed by Trypan blue exclusion and flow cytometry respectively. Monocyte composition and membrane remodeling were studied using gas chromatography and NR12S staining. Microparticles released in supernatant were measured by prothrombinase assay. After LPS challenge, both cellular necrosis and apoptosis were increased (threefold and twofold respectively) and microparticle release was enhanced (sevenfold) after supplementation with Medialipid(®) compared to Intralipid(®), SMOFlipid(®) and monocytes in the standard medium. The monocytes differentially incorporated fatty acids after lipid emulsion challenge. Finally, lipid-treated cells displayed microparticles characterized by disrupted membrane lipid order, reflecting lipid remodeling of the parental cell plasma membrane. Our data suggest that lipid emulsions differentially alter cell viability, monocyte composition and thereby microparticle release. While MCT have deleterious effects, we have shown that parenteral nutrition emulsion containing LCT or LCT and MCT associated to n-3 and n-9 fatty acids have no effect on endotoxin-induced cell death and inflammation. PMID:25038627

  13. Preventive Effect of Berberis Integerrima on the Serum Levels of Glucose and Lipids in Streptozotocin (STZ-Induced Diabetes in Rats

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    Hossein Ashraf

    2012-12-01

    Full Text Available Background and Objective: Use of medicinal plants for attenuation of hyperglycemia and restoration of lipids to normal level is clinically very important. We decided to assess the preventive role of aqueous extract of Berberis Integerrima root on the serum levels of glucose, insulin, and lipid profile in Streptozotocin (STZ-induced diabetic rats.Materials and Methods: Forty male rats were divided into 5 groups as follows: 1- normal (N; 2- normal + barberry (N+B (they received barberry root extract for 6 weeks; 3- diabetic (D (they received STZ, 65 mg/kg BW /i.p.; 4- diabetic + barberry before (D+Bb (they received barberry root extract for 3 weeks before STZ injection and continued for another three weeks; and 5- diabetic + barberry after (D+Ba (three days after STZ injection, they received barberry root extract for 3 weeks. The experimental groups received barberry root extract (500 mg/kg bw intra gastric by gavage for 6 weeks and the experimental period for each rat was 6 weeks.Results: Diabetic rats showed a significant increase in serum glucose, total cholesterol, triglycerides, LDL-C, and food intake as well as a decrease in HDL-C, body weight and serum insulin, compared to the other groups. Administration of the barberry root extract in diabetic rats restored these changes towards normal to some extent.Conclusion: In this study, for the first time, we showed that the administration of the barberry root extract before diabetes induction resulted in better amelioration in the serum levels of glucose, insulin, and lipid profile, compared to the group receiving it after induction: this indicates that the barberry root extract can play both a preventive and a therapeutic role in such patients.

  14. Effects of canola oil supplemented with atherogenic element and nigella sativa (kalonji) on serum lipids in albino rats - an experimental study

    International Nuclear Information System (INIS)

    To compare effects of canola oil supplemented with atherogenic element and Nigella sativa on serum lipids in albino rats. Place and Duration of Study: Study was conducted at Pathology Department of Postgraduate Medical Institute, for 12 weeks. Study Design: Laboratory based randomized controlled trials. Material and Methods: Seventy two albino rats were selected and randomly divided into six groups of twelve animals with equal number of male and female in each. Fourteen days after acclimatization to the environment and basal diet, fasting blood samples (zero week) were collected by heart puncture under ether anesthesia and experimental diets were started which were continued for 12 weeks. All parameters were measured using enzymatic colorimetric methods. Results: Estimations of serum lipids showed increase in total cholesterol (TC) and High Density Lipoprotein Cholesterol (HDL-c) levels but fall in LDL-c concentrations in groups fed on canola oil diet. On the other hand, even atherogenic supplemented groups had decrease in cardio-protective HDL-c and raised LDL-c; although statistically non-significant. Thus canola oil diets were not hyperlipidaemic and prevented adiposity. Nigella sativa (NS) diets significantly decreased serum total cholesterol and LDL-c while HDL-c was raised but non-significantly. Thus Nigella sativa prevented deposition of lipids in tissues, thus preventing tendency to obesity and atherogenesis by decreasing LDL-c in serum. Conclusion: Nigella sativa produces antilipidaemic and anti-obesity effects by decreasing low density lipoprotein cholesterol level which is statistically significant in two out of the three groups fed on Ns; it also increased high density cholesterol which was however non-significant in comparison with Canola oil alone. (author)

  15. The influence of a thyroxine supplemented diet on selected hepatic redox equilibrium markers, liver morphology and the serum lipid profile in rats treated with doxorubicin 

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    Agnieszka Korga

    2013-03-01

    Full Text Available Introduction: Cytotoxicity of doxorubicin (DOX – an anticancer drug, mostly results from reactive oxygen species (ROS generation. Some enzymes catalyzing this process and enzymes of antioxidant defense are regulated by iodothyronine hormones. Thus, disorders in iodothyronine hormone status may affect doxorubicin-induced redox imbalance and anabolic/catabolic disorders. The aim of this study was to evaluate the influence of doxorubicin and thyroxine (T4 associated treatment on liver morphology, markers of oxidative stress and plasma lipid parameters.Materials and methods: Rats were intraperitoneally treated with doxorubicin (1.5 mg/kg once a week for ten weeks. Thyroxine was simultaneously given in drinking water (0.2 or 2.0 mg/l for 14 weeks.Results: There were higher hepatic level of malonyldialdehyde (MDA of all tested groups and at the same time in rats treated with DOX plus T4 lower concentrations of total glutathione compared to controls were observed. Morphology of liver did not show any features of necrosis or steatosis but a decrease of glycogen content in T4 DOX groups compared to DOX treatment was observed. The concomitant administration of a lower dose of thyroxine and doxorubicin decreased triglycerides (TG and increased LDL level compared to the DOX group.Discussion: Thyroxin supplementation caused redox equilibrium disorders and oxidative stress in liver of rats receiving DOX. The study revealed the normalizing influence of thyroxin on glycogen deposits that were observed after doxorubicin treatment. Apart from an adverse impact of thyroxine administration on LDL in rats treated with doxorubicin, a beneficial effect of lower dose of thyroxine on serum TG level was revealed. 

  16. Conversion from fluvastatin to simvastatin therapy at a dose ratio of 8 to 1: effect on serum lipid levels and cost.

    Science.gov (United States)

    Rindone, J P; Arriola, G

    1998-01-01

    The objective of this study was to assess the effect on serum lipid levels of the substitution of simvastatin for fluvastatin at a dose ratio of 8:1 (fluvastatin to simvastatin). A secondary objective was to determine the number of patients at goal lipid levels before and after this substitution. The study included 60 outpatients with hyperlipidemia who had received a constant dose of fluvastatin for at least 6 weeks. After a baseline 12-hour lipid profile (total cholesterol, triglycerides, high-density lipoprotein, and low-density lipoprotein [LDL]) was obtained, patients were switched from fluvastatin to simvastatin at an 8:1 dose ratio. Patients were instructed to split the simvastatin tablets in half with a pill splitter and to take one half-tablet at bedtime. A repeat lipid profile and liver function testing were performed after 6 to 8 weeks of simvastatin therapy. Lipid components were compared before and during simvastatin therapy using a paired t test. Target LDL levels were based on guidelines issued by the National Cholesterol Education Program. Fifty-six patients completed the study. No change in lipid components was observed, except for a statistically significant decrease in LDL. The majority of patients had a decrease in LDL levels, rather than an increase, after the conversion to simvastatin. Six patients required a dose increase of simvastatin in response to increased LDL levels. Forty-one percent of patients achieved goal LDL levels with simvastatin, compared with 30% with fluvastatin. Four patients withdrew from the study, two because of troublesome side effects and two for failure to complete the protocol. The results show that simvastatin can be substituted for fluvastatin at a dose ratio of 8:1 without loss of lipid control in the majority of patients and that by using this ratio and splitting tablets, significant cost savings can be realized. PMID:9589824

  17. Phenolic Lipids Affect the Activity and Conformation of Acetylcholinesterase from Electrophorus electricus (Electric eel)

    OpenAIRE

    Maria Stasiuk; Alicja Janiszewska; Arkadiusz Kozubek

    2014-01-01

    Phenolic lipids were isolated from rye grains, cashew nutshell liquid (CNSL) from Anacardium occidentale, and fruit bodies of Merrulius tremellosus, and their effects on the electric eel acetylcholinesterase activity and conformation were studied. The observed effect distinctly depended on the chemical structure of the phenolic lipids that were available for interaction with the enzyme. All of the tested compounds reduced the activity of acetylcholinesterase. The degree of inhibition varied, ...

  18. Do abnormal serum lipid levels increase the risk of chronic low back pain? The Nord-Trondelag Health Study.

    Directory of Open Access Journals (Sweden)

    Ingrid Heuch

    Full Text Available Cross-sectional studies suggest associations between abnormal lipid levels and prevalence of low back pain (LBP, but it is not known if there is any causal relationship.The objective was to determine, in a population-based prospective cohort study, whether there is any relation between levels of total cholesterol, high density lipoprotein (HDL cholesterol and triglycerides and the probability of experiencing subsequent chronic (LBP, both among individuals with and without LBP at baseline.Information was collected in the community-based HUNT 2 (1995-1997 and HUNT 3 (2006-2008 surveys of an entire Norwegian county. Participants were 10,151 women and 8731 men aged 30-69 years, not affected by chronic LBP at baseline, and 3902 women and 2666 men with LBP at baseline. Eleven years later the participants indicated whether they currently suffered from chronic LBP.Among women without LBP at baseline, HDL cholesterol levels were inversely associated and triglyceride levels positively associated with the risk of chronic LBP at end of follow-up in analyses adjusted for age only. Adjustment for the baseline factors education, work status, physical activity, smoking, blood pressure and in particular BMI largely removed these associations (RR: 0.96, 95% CI: 0.85-1.07 per mmol/l of HDL cholesterol; RR: 1.16, 95% CI: 0.94-1.42 per unit of lg(triglycerides. Total cholesterol levels showed no associations. In women with LBP at baseline and men without LBP at baseline weaker relationships were observed. In men with LBP at baseline, an inverse association with HDL cholesterol remained after complete adjustment (RR: 0.83, 95% CI: 0.72-0.95 per mmol/l.Crude associations between lipid levels and risk of subsequent LBP in individuals without current LBP are mainly caused by confounding with body mass. However, an association with low HDL levels may still remain in men who are already affected and possibly experience a higher pain intensity.

  19. Serum From Advanced Heart Failure Patients Promotes Angiogenic Sprouting and Affects the Notch Pathway in Human Endothelial Cells.

    Science.gov (United States)

    Pannella, Micaela; Caliceti, Cristiana; Fortini, Francesca; Aquila, Giorgio; Vieceli Dalla Sega, Francesco; Pannuti, Antonio; Fortini, Cinzia; Morelli, Marco Bruno; Fucili, Alessandro; Francolini, Gloria; Voltan, Rebecca; Secchiero, Paola; Dinelli, Giovanni; Leoncini, Emanuela; Ferracin, Manuela; Hrelia, Silvana; Miele, Lucio; Rizzo, Paola

    2016-12-01

    It is unknown whether components present in heart failure (HF) patients' serum provide an angiogenic stimulus. We sought to determine whether serum from HF patients affects angiogenesis and its major modulator, the Notch pathway, in human umbilical vein endothelial cells (HUVECs). In cells treated with serum from healthy subjects or from patients at different HF stage we determined: (1) Sprouting angiogenesis, by measuring cells network (closed tubes) in collagen gel. (2) Protein levels of Notch receptors 1, 2, 4, and ligands Jagged1, Delta-like4. We found a higher number of closed tubes in HUVECs treated with advanced HF patients serum in comparison with cells treated with serum from mild HF patients or controls. Furthermore, as indicated by the reduction of the active form of Notch4 (N4IC) and of Jagged1, advanced HF patients serum inhibited Notch signalling in HUVECs in comparison with mild HF patients' serum and controls. The circulating levels of NT-proBNP (N-terminal of the pro-hormone brain natriuretic peptide), a marker for the detection and evalutation of HF, were positively correlated with the number of closed tubes (r = 0.485) and negatively with Notch4IC and Jagged1 levels in sera-treated cells (r = -0.526 and r = -0.604, respectively). In conclusion, we found that sera from advanced HF patients promote sprouting angiogenesis and dysregulate Notch signaling in HUVECs. Our study provides in vitro evidence of an angiogenic stimulus arising during HF progression and suggests a role for the Notch pathway in it. J. Cell. Physiol. 231: 2700-2710, 2016. © 2016 Wiley Periodicals, Inc. PMID:26987674

  20. Associations between the human intestinal microbiota, Lactobacillus rhamnosus GG and serum lipids indicated by integrated analysis of high-throughput profiling data

    OpenAIRE

    Leo Lahti; Anne Salonen; Kekkonen, Riina A.; Jarkko Salojärvi; Jonna Jalanka-Tuovinen; Airi Palva; Matej Orešič; Willem M. de Vos

    2013-01-01

    Accumulating evidence indicates that the intestinal microbiota regulates our physiology and metabolism. Bacteria marketed as probiotics confer health benefits that may arise from their ability to affect the microbiota. Here high-throughput screening of the intestinal microbiota was carried out and integrated with serum lipidomic profiling data to study the impact of probiotic intervention on the intestinal ecosystem, and to explore the associations between the intestinal bacteria and serum li...

  1. Effects of the Polysaccharide from the Sporophyll of Brown Alga Undaria Pinnatifida on Serum Lipid Profile and Fat Tissue Accumulation in Rats Fed a High-Fat Diet.

    Science.gov (United States)

    Kim, Byoung-Mok; Park, Jae-Ho; Kim, Dong-Soo; Kim, Young-Myung; Jun, Joon-Young; Jeong, In-Hak; Chi, Young-Min

    2016-07-01

    We investigated the effects of the polysaccharide from the sporophyll of a selected brown alga Undaria pinnatifida on serum lipid profile, fat tissue accumulation, and gastrointestinal transit time in rats fed a high-fat diet. The algal polysaccharide (AP) was prepared by the treatment of multiple cellulase-producing fungi Trichoderma reesei and obtained from the sporophyll with a yield of 38.7% (dry basis). The AP was mostly composed of alginate and fucoidan (up to 89%) in a ratio of 3.75:1. The AP was added to the high-fat diet in concentrations of 0.6% and 1.7% and was given to male Sprague-Dawley rats (5-wk-old) for 5 wk. The 1.7% AP addition notably reduced body weight gain and fat tissue accumulation, and it improved the serum lipid profile, including triglycerides, total cholesterol, and very low-density lipoprotein-cholesterol. The effects were associated with increased feces weight and shortened gastrointestinal transit time. In addition, the lipid peroxidation of the liver was decreased in both groups. PMID:27384013

  2. Effect of black pepper (piper nigrum) on body weight and some serum lipid fractions in whole body gamma irradiated albino rats

    International Nuclear Information System (INIS)

    Two experiments were conducted to determine effect of sublethal body gamma irradiation (6.0 Gy as a single dose) on body weight and some serum lipid fractions in female and male rats. The beneficial efficacy of intragastric administration of black pepper (in two doses of 160 and 320 mg/kg bw) was also investigated. The results obtained revealed that the sublethal irradiated rats showed a drastic loss in body weight reached 39.5% less than the normal males and significant alterations in serum triglycerides, cholesterol, high density lipoprotein (HDL-cholesterol) and low density lipoprotein (LDL-cholesterol) levels, 5 weeks post exposure to gamma irradiation in female and male rats. Black pepper showed a beneficial effect on these significant changes in lipid fractions in irradiated female and male rats. Treatment with the two doses of black pepper up to 14 weeks, 5 days/week, showed a slight effect on body gain and fluctuations in the lipid fractions at 5.10 and 14 weeks for both sexes

  3. The Effect of Chlorella vulgaris Supplementation on Liver Enzymes, Serum Glucose and Lipid Profile in Patients with Non-Alcoholic Fatty Liver Disease

    Directory of Open Access Journals (Sweden)

    Mehrangiz Ebrahimi-Mameghani

    2014-07-01

    Full Text Available Background: Non-alcoholic fatty liver disease (NAFLD is becoming a public health problem worldwide and using microalgae is a new approach on its treatment. The aim of this study was to investigate the effect of Chlorella vulgaris supplementation on liver enzymes, serum glucose and lipid profile in patients with NAFLD. Methods: This double-blind randomized placebo-controlled clinical trial was conducted on 60 NAFLD patients from specialized clinics of Tabriz University of Medical Sciences from December 2011 to July 2012. The subjects were randomly allocated into 2 groups: 1 “intervention” (n=30 received 400 mg/day vitamin E plus four 300 mg tablets of Chlorella vulgaris and, 2 “placebo” (n=30 received 400 mg/day vitamin E and four placebo tablets per day for 8 weeks. Weight, liver enzymes and metabolic factors were assessed in fasting serum and dietary data was collected at baseline and end of the study. Results: Weight, liver enzymes, fasting blood sugar (FBS and lipid profile decreased significantly in both groups (P<0.05. The differences in weight, ALP and FBS between the two groups were statistically significant (P=0.01, P=0.04 and P=0.02, respectively. Conclusion: C. vulgaris seems to improve FBS and lipid profile and therefore could be considered as an effective complementary treatment in NAFLD.

  4. Influence of diet with kale on lipid peroxides and malondialdehyde levels in blood serum of laboratory rats over intoxication with paraquat

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    Elżbieta Sikora

    2013-03-01

    Full Text Available Background. Organism’s lipid peroxidation is one of the most often examined and known physiological process evoked by free radicals. It concerns oxidation reaction of unsaturated fatty acid and/or other lipids leading to lipid oxidation products (LOP, which as a result of further changes generate among others the malondialdehyde molecules. The aim of the work was an estimation if raw or cooked kale addition to rat’s diet infl uences antioxidant defense effi ciency in their organisms in comparison to rats fed with standard AIN-93G diet. Material and methods. The experiment was conducted with 36 Wistar strain, male rats over 21 days. The rats were divided into 3 groups (each 12 stuck which were fed with: standard diet AIN-93G (2 groups, AIN-93G diet with 10% addition of raw kale (2 groups, and AIN-93G with 10% addition of cooked lyophilised kale. The total content of polyphenols (FC method and antioxidant activity (ABTS+• were previously determined in raw and then in cooked kale. On the 20th day of experiment, half of rats (6 stuck of each kind of the diet were injected intraperitoneally by the solution of paraquat (PQ in physiological salt to evoke the oxidative stress. The next day animals were stunned and blood from their hearts was sampled. In the obtained serum, the levels of lipid oxidation products (LOP and malondialdehyde (MDA were assessed. Results. It was observed that in blood serum of rats fed with modifi ed diet with raw and cooked lyophilised kale addition the lipid oxides level was lower in comparison to control group fed with standard diet (p < 0.05. It was found that intoxication with paraquat caused growth of MDA and LOP levels in blood serum of all rats in comparison to not intoxicated groups but that growth was the lowest in group fed diet with cooked kale addition. Conclusion. Diet with kale, both raw and cooked, effi ciently inhibited the lipid peroxidation process in rats’ organisms, ongoing during natural metabolism

  5. Insulin Resistance and Obesity Affect Lipid Profile in the Salivary Glands

    Science.gov (United States)

    Matczuk, Jan; Zalewska, Anna; Łukaszuk, Bartłomiej; Knaś, Małgorzata; Maciejczyk, Mateusz; Garbowska, Marta; Ziembicka, Dominika M.; Waszkiel, Danuta; Chabowski, Adrian; Żendzian-Piotrowska, Małgorzata

    2016-01-01

    In today's world wrong nutritional habits together with a low level of physical activity have given rise to the development of obesity and its comorbidity, insulin resistance. More specifically, many researches indicate that lipids are vitally involved in the onset of a peripheral tissue (e.g., skeletal muscle, heart, and liver) insulin resistance. Moreover, it seems that diabetes can also induce changes in respect of lipid composition of both the salivary glands and saliva. However, judging by the number of research articles, the salivary glands lipid profile still has not been sufficiently explored. In the current study we aim to assess the changes in the main lipid fractions, namely, triacylglycerols, phospholipids, free fatty acids, and diacylglycerols, in the parotid and the submandibular salivary glands of rats exposed to a 5-week high fat diet regimen. We observed that the high caloric fat diet caused a significant change in the salivary glands lipid composition, especially with respect to PH and TG, but not DAG or FFAs, classes. The observed reduction in PH concentration is an interesting phenomenon frequently signifying the atrophy and malfunctions in the saliva secreting organs. On the other hand, the increased accumulation of TG in the glands may be an important clinical manifestation of metabolic syndrome and type 2 diabetes mellitus. PMID:27471733

  6. Insulin Resistance and Obesity Affect Lipid Profile in the Salivary Glands

    Directory of Open Access Journals (Sweden)

    Jan Matczuk

    2016-01-01

    Full Text Available In today’s world wrong nutritional habits together with a low level of physical activity have given rise to the development of obesity and its comorbidity, insulin resistance. More specifically, many researches indicate that lipids are vitally involved in the onset of a peripheral tissue (e.g., skeletal muscle, heart, and liver insulin resistance. Moreover, it seems that diabetes can also induce changes in respect of lipid composition of both the salivary glands and saliva. However, judging by the number of research articles, the salivary glands lipid profile still has not been sufficiently explored. In the current study we aim to assess the changes in the main lipid fractions, namely, triacylglycerols, phospholipids, free fatty acids, and diacylglycerols, in the parotid and the submandibular salivary glands of rats exposed to a 5-week high fat diet regimen. We observed that the high caloric fat diet caused a significant change in the salivary glands lipid composition, especially with respect to PH and TG, but not DAG or FFAs, classes. The observed reduction in PH concentration is an interesting phenomenon frequently signifying the atrophy and malfunctions in the saliva secreting organs. On the other hand, the increased accumulation of TG in the glands may be an important clinical manifestation of metabolic syndrome and type 2 diabetes mellitus.

  7. Prediction of non-alcoholic fatty-liver disease and liver fat content by serum molecular lipids

    DEFF Research Database (Denmark)

    Orešic, Matej; Hyötyläinen, Tuulia; Kotronen, Anna;

    2013-01-01

    We examined whether analysis of lipids by ultra-performance liquid chromatography (UPLC) coupled to MS allows the development of a laboratory test for non-alcoholic fatty-liver disease (NAFLD), and how a lipid-profile biomarker compares with the prediction of NAFLD and liver-fat content based on ...

  8. Serum IGF-1 affects skeletal acquisition in a temporal and compartment-specific manner.

    Directory of Open Access Journals (Sweden)

    Hayden-William Courtland

    Full Text Available Insulin-like growth factor-1 (IGF-1 plays a critical role in the development of the growing skeleton by establishing both longitudinal and transverse bone accrual. IGF-1 has also been implicated in the maintenance of bone mass during late adulthood and aging, as decreases in serum IGF-1 levels appear to correlate with decreases in bone mineral density (BMD. Although informative, mouse models to date have been unable to separate the temporal effects of IGF-1 depletion on skeletal development. To address this problem, we performed a skeletal characterization of the inducible LID mouse (iLID, in which serum IGF-1 levels are depleted at selected ages. We found that depletion of serum IGF-1 in male iLID mice prior to adulthood (4 weeks decreased trabecular bone architecture and significantly reduced transverse cortical bone properties (Ct.Ar, Ct.Th by 16 weeks (adulthood. Likewise, depletion of serum IGF-1 in iLID males at 8 weeks of age, resulted in significantly reduced transverse cortical bone properties (Ct.Ar, Ct.Th by 32 weeks (late adulthood, but had no effect on trabecular bone architecture. In contrast, depletion of serum IGF-1 after peak bone acquisition (at 16 weeks resulted in enhancement of trabecular bone architecture, but no significant changes in cortical bone properties by 32 weeks as compared to controls. These results indicate that while serum IGF-1 is essential for bone accrual during the postnatal growth phase, depletion of IGF-1 after peak bone acquisition (16 weeks is compartment-specific and does not have a detrimental effect on cortical bone mass in the older adult mouse.

  9. Melatonin affects the order, dynamics and hydration of brain membrane lipids

    Science.gov (United States)

    Akkas, Sara B.; Inci, Servet; Zorlu, Faruk; Severcan, Feride

    2007-05-01

    The brain is especially susceptible to free radical attack since it is rich in polyunsaturated fatty acids and consumes very high amounts of oxygen. Melatonin is a non-enzymatic amphiphilic antioxidant hormone that is widely used in medicine for protective and treatment purposes in cases of oxidative stress. In the present work, the effects of the clinically used dose of melatonin (a single intraperitoneal dose of 100 mg/kg) on rat brain homogenate were investigated as a function of temperature using Fourier transform infrared spectroscopy. The results showed that the lipid to protein ratio decreases in the melatonin treated brain samples. Moreover, it is revealed that melatonin disorders and decreases the dynamics of lipids and induces a strengthening in the hydrogen bonding between the functional groups of both melatonin and the polar parts of lipids and/or water at physiological temperatures.

  10. Fish oil affects blood pressure and the plasma lipid profile in healthy Danish infants

    DEFF Research Database (Denmark)

    Damsgaard, C.T.; Schack-Nielsen, L.; Michaelsen, K.F.; Fruekilde, Maj-Britt; Hels, O.; Lauritzen, L.

    2006-01-01

    Animal and epidemiologic studies indicate that early nutrition has lasting effects on metabolism and cardiovascular disease risk. In adults, (n-3) long-chain PUFA (LCPUFA) from fish oils improve blood pressure, the lipid profile, and possibly cardiovascular disease mortality. This randomized trial...... is the first to investigate the effects of fish oil on blood pressure and the lipid profile in infancy. Healthy term 9-mo old infants In 83) were randomly assigned to 5 mL fish oil daily or no fish oil for 3 mo and to 2 different milk types. Before and after the intervention, blood pressure was...... measured with an oscillometric device, and blood was sampled for analysis of erythrocyte fatty acid composition and the plasma lipid profile. This paper examines the effects of the fish oil supplement, with adjustment for the effects of the milk intervention when relevant. The fish oil intervention...

  11. Does the antibody production ability affect the serum anti-Helicobacter pylori IgG titer?

    Science.gov (United States)

    Chung, Hyun Ah; Lee, Sun-Young; Moon, Hee Won; Kim, Jeong Hwan; Sung, In-Kyung; Park, Hyung Seok; Shim, Chan Sup; Han, Hye Seung

    2016-01-01

    AIM To investigate the relationship between serum titers of anti-Helicobacter pylori (H. pylori) immunoglobulin G (IgG) and hepatitis B virus surface antibody (HBsAb). METHODS Korean adults were included whose samples had positive Giemsa staining on endoscopic biopsy and were studied in the hepatitis B virus surface antigen (HBsAg)/HBsAb serologic assay, pepsinogen (PG) assay, and H. pylori serologic test on the same day. Subjects were excluded if they were positive for HBsAg, had a recent history of medication, or had other medical condition(s). We analyzed the effects of the following factors on serum titers of HBsAb and the anti-H. pylori IgG: Age, density of H. pylori infiltration in biopsy samples, serum concentrations of PG I and PG II, PG I/II ratio, and white blood cell count. RESULTS Of 111 included subjects, 74 (66.7%) exhibited a positive HBsAb finding. The serum anti-H. pylori IgG titer did not correlate with the serum HBsAb titer (P = 0.185); however, it correlated with the degree of H. pylori infiltration on gastric biopsy (P < 0.001) and serum PG II concentration (P = 0.042). According to the density of H. pylori infiltration on gastric biopsy, subjects could be subdivided into those with a marked (median: 3.95, range 0.82-4.00) (P = 0.458), moderate (median: 3.37, range 1.86-4.00), and mild H. pylori infiltrations (median: 2.39, range 0.36-4.00) (P < 0.001). Subjects with a marked H. pylori infiltration on gastric biopsy had the highest serological titer, whereas in subjects with moderate and mild H. pylori infiltrations titers were correspondingly lower (P < 0.001). After the successful eradication, significant decreases of the degree of H. pylori infiltration (P < 0.001), serum anti-H. pylori IgG titer (P < 0.001), and serum concentrations of PG I (P = 0.028) and PG II (P = 0.028) were observed. CONCLUSION The anti-H. pylori IgG assay can be used to estimate the burden of bacteria in immunocompetent hosts with H. pylori infection, regardless

  12. 1,8-cineole protected human lipoproteins from modification by oxidation and glycation and exhibited serum lipid-lowering and anti-inflammatory activity in zebrafish

    Directory of Open Access Journals (Sweden)

    Kyung-Hyun Cho1,2,*

    2012-10-01

    Full Text Available We recently reported that a water extract of laurel or turmeric,1,8-cineole enriched fractions, showed hypolipidemic activityin the zebrafish model. Therefore, the present study investigatedthe cineole’s anti-oxidant and anti-inflammatory activitiesin lipoprotein metabolism in vitro and in vivo. Cineolehad inhibitory effects on cupric ion-mediated oxidation of lipoproteinsin general, while simultaneously enhancing ferric ionremoval ability in high-density lipoprotein (HDL. Hypercholesterolemiawas induced in zebrafish using cholesterol-feedingtreatment, 4% cholesterol, for 3 weeks. After feeding with orwithout the addition of cineole, the results revealed that cineolepossessed lipid-lowering and anti-inflammatory activitiesin hypercholesterolemic zebrafish. In addition, serum amyloidA and interleukin-6 levels were lowered and lipid accumulationwas decreased in the liver. Conclusively, 1,8-cineole wasfound to have anti-oxidant activities in lipoprotein metabolismboth in vitro and in vivo with simultaneous reduction of lipidaccumulation in the liver of zebrafish.

  13. 体位改变对血脂检测指标的影响%Effect of body positions on serum lipid parameters

    Institute of Scientific and Technical Information of China (English)

    龙聪; 明亮; 杨章元

    2011-01-01

    Objective To investigate the effect of body positions on serum lipid parameters. Methods By autogenous contrast and cross match survey, blood samples of 100 volunteers were drawn from the same part in both standing and lying positions. 25 of the volunteers had their blood samples collected respectively after standing and sitting for IS min, lying for 15 min and 30 min and then sitting for another 15 min. The blood samples accompanied with quality control materials were analyzed for eight serum lipid parameters on an automatic biochemical analyzer. Results Setting the lying position as baseline, the eight serum lipid parameters detected in sitting position were significantly higher than those in lying position (P0.05). The average increase rate was 11.71% and the highest increase rate was 22.95%. The parameters showed no correlation with gender. In the 25 volunteers, the parameters increased successively from lying, sitting to standing position. While sitting for 15 min after lying, these parameters returned to 95 % of the original parameters in sitting position. Conclusion Changing body position can result in obvious physiological variation of the eight serum lipid parameters.%目的 探讨不同体位对血脂检测指标的影响.方法 100名志愿者,经同体交叉配对设计,抽取坐、卧位同一部位的血液,其中25名分别抽站15min、坐15min、卧15min、30 min、卧后坐15min标本,在全自动生化分析仪上与质控品同时检测8项血脂指标.结果 以卧位为基线与坐位相比,8项血脂检测结果平均升高11.71%,最高升至22.95%,差异均有统计学意义(P<0.05),且与性别有关.25名志愿者血脂水平从卧位到站位结果逐渐升高,依次为卧位<坐位<站位.卧位后坐15min,各指标可恢复到原坐位的95%以上.结论 体位改变能引起血脂检测指标的显著差异.

  14. Related research of blood glucose, blood lipid and serum cystatin C and lower-extremity arterial disease in type 2 diabetic mellitus

    Institute of Scientific and Technical Information of China (English)

    Juan Zeng; Jie Ou; Yun-Mei He; Chun-Yu Cai

    2016-01-01

    Objective:To explore the relationship between levels of blood glucose, blood lipid and serum cystatin C and lower-extremity arterial disease in type 2 diabetic mellitus so as to provide the basis for the prevention and treatment of the disease.Methods:A total of 240 cases of patients with type 2 diabetic mellitus receiving physical examinations in our hospital from March 2014 to March 2015 were selected and divided into three groups. The control group was consisted of type 2 diabetes patients without lower-extremity arterial disease, the observation group 1 included type 2 diabetes patients with mild or moderate lower-extremity arterial disease, and the observation group 2 was formed by type 2 diabetes patients with severe lower-extremity arterial disease. Each group contained 80 cases. Then, the heights, weights, waist and hip circumferences of patients in the three groups were determined and the body mass index (BMI) and waist to hip ratio (WHR) were calculated; the fasting blood glucose (FBG), fasting insulin (FINS), insulin resistance index (HOMA-IR), 2 h postprandial blood glucose (2 h PG) and glycosylated hemoglobin (HBA1c) in two groups were detected; and the levels of serum total cholesterol(TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), apolipoprotein A1 (apoA1), apolipoprotein B (apoB) and cystatin C (CysC) in two groups were also tested.Results:Compared with the control group, the WHR, FBG, HOMA-IR, 2 h PG, HBA1c, LDL-C, apoB and CysC all increased significantly, and the differences were statistically significant. Besides, there were statistically significances existing between the observation groups 1 and 2. What’s more, the serum levels of TC, HDL-C, TG and apoA1 in the observation groups were not significantly different from those of the control group.Conclusions: There is a close relationship between blood glucose, blood lipid, serum cystatin C level and lower-extremity arterial

  15. The Relationship Between Carotenoids Intake and Serum Lipids in Adults%成人膳食中类胡萝卜素摄入量与血脂关系

    Institute of Scientific and Technical Information of China (English)

    杨艳晖; 常东; 潘洪志; 陈文华

    2012-01-01

    目的 研究成人膳食中类胡萝卜素的摄入状况与血脂的关系. 方法 采用问卷调查法,调查130名35~60岁人群连续3d的膳食情况,根据膳食摄人情况计算5种类胡萝卜素(包括α-胡萝卜素、β-胡萝卜素、β-隐黄质、番茄红素、叶黄素/玉米黄质)每此摄入量,将调查人群按类胡萝卜素摄入量四分位间距分组,观察类萝卜素的摄入量与血脂的关系. 结果 第1组人群血清甘油三酯水平明显低于第4组人群,差异有统计学意义(P<0.05).相关分析结果表明,类胡萝卜素的摄入量与血清中甘油三酯含量呈显著负相关(P<0.05). 结论 结果提示成人膳食类胡萝卜素的摄入与甘油三酯水平具有相关性,摄入类胡萝卜素可能对血脂有一定程度的保护作用.%Objective To investigate the relationship between carotenoids intake and serum lipids in adults. Methods Dietary intake for a consecution of 3 days was investigated in 130 objects aged 35 to 60 years old by a questionnaire. Daily Intake of five carotenoids, including α-carotene, β- carotene, β- cryptoxanthin, lycopene and lutein/zeaxanthin, were calculated. The investigation objects were classified into four groups according to the quartiles of the carotenoids intake: Group 1 (P100 - P75), Group 2 (P75 - P50), Group 3 (P50 - P25), Group 4 (P25 - P0). The relationship between carotenoids intake and serum lipids was explored. Results The serum triglyceride level in Group 1 was significantly lower than that in Group 4 (P<0.05). There was a negative correlation between the carotenoids intake and serum triglyceride level. Conclusions Serum triglyceride level in adult is correlated with the dietary intake of carotenoids. Carotenoids intake might protect lipids increase to some extent.

  16. Changes in serum lipids in patients with rheumatoid arthritis treated with a combination of tocilizumab and methotrexate compared with methotrexate alone for 24 weeks of observation

    Directory of Open Access Journals (Sweden)

    E. V. Udachkina

    2015-11-01

    Full Text Available Background. According to the some studies tocilizumab therapy (TCZ in patients with rheumatoid arthritis (RA is accompanied by deterioration of blood lipid profile. Aim. To study changes in serum lipid parameters in patients with RA treated with a combination of tocilizumab and methotrexate compared with methotrexate alone for 24 weeks of observation. Material and methods. Patients (n=72 with RA were included into the pilot non-randomized 24-week study and divided in two groups: 1 TCZ+MTX group (n=39; women 30; median age 51 [43-55] years; 6 i.v. infusions of TCZ 8 mg/kg + МТX 10-20 mg/week; 2 MTX group (n=33; women 23; mеdian age 56 [48-63] years; MTX 7.5-20 mg/week. Results. At the baseline, similar proatherogenic blood profile was observed in both groups. The patients of MTX group more frequently took statins (n=19; 57.6% compared with the group TCZ+MTX (n=7; 18%, (p<0.05. The lipid levels correlated positively with traditional risk factors (p<0.05. RA activity and duration correlated negatively with high density lipoprotein cholesterol (HDL-C, (p<0.05. Good/satisfactory anti-inflammatory effect was achieved in both groups after 24 weeks of treatment. Patients of TCZ+MTX group showed an increase in total cholesterol and HDL-C levels by 11% and 110%, respectively and decrease in plasma atherogenic index (PAI by 47%, (p<0.05. HDL-C level increased by 22% and PAI decreased by 16% in patients of MTX group (p<0.05. Among patients of MTX group without statin therapy HDL-C as well as non-HDL-C levels were increased by 24% and 27%, respectively (p<0.05; PAI did not change significantly in this subgroup. Among patients of MTX group treated with statins isolated increase in HDL-C level by 22% and decrease in PAI by 37.3% (p<0.05 were observed. A number of patients with achieved target levels of all studied lipid parameters did not change significantly in both groups. Conclusions. TCZ+MTX combined therapy as well as MTX monotherapy are associated

  17. Dietary ɛ-Polylysine Decreased Serum and Liver Lipid Contents by Enhancing Fecal Lipid Excretion Irrespective of Increased Hepatic Fatty Acid Biosynthesis-Related Enzymes Activities in Rats

    OpenAIRE

    HOSOMI, Ryota; Yamamoto, Daiki; Otsuka, Ren; Nishiyama, Toshimasa; Yoshida, Munehiro; Fukunaga, Kenji

    2015-01-01

    ɛ-Polylysine (EPL) is used as a natural preservative in food. However, few studies have been conducted to assess the beneficial functions of dietary EPL. The purpose of this study was to elucidate the mechanism underlying the inhibition of neutral and acidic sterol absorption and hepatic enzyme activity-related fatty acid biosynthesis following EPL intake. EPL digest prepared using an in vitro digestion model had lower lipase activity and micellar lipid solubility and higher bile acid binding...

  18. Is the fact of parenting couples cohabitation affecting the serum levels of persistent organohalogen pollutants?

    DEFF Research Database (Denmark)

    Góralczyk, Katarzyna; Struciński, Paweł; Wojtyniak, Bogdan;

    2015-01-01

    Organohalogen compounds constitute one of the important groups of persistent organic pollutants (POPs). Among them, due to their long-term health effects, one should pay attention on organochlorine pesticides, polychlorinated biphenyls (PCBs) and perfluoroalkylated substances (PFASs). This paper is...... results show that in case of all compounds under study the highest concentrations were present always in men in relation to the levels detected in the blood serum of their female partners, regardless of the country of origin of the couple. A positive correlation was noted between the concentrations of the...... studied compounds in the blood serum of men and women in parenting couples. In some cases these correlations were statistically significant, e.g. for concentrations of p,p'-DDE in pairs from Greenland and Ukraine, of CB-153 in pairs from Poland and Ukraine, and of PFOS for parents from Greenland and...

  19. Size and molecular flexibility affect the binding of ellagitannins to bovine serum albumin

    OpenAIRE

    Dobreva, Marina; Green, R. J.; Mueller-Harvey, Irene; Salminen, J.-P.; Howlin, B.J.; Frazier, Richard

    2014-01-01

    Binding to bovine serum albumin of monomeric (vescalagin and pedunculagin) and dimeric ellagitannins (roburin A, oenothein B, and gemin A) was investigated by isothermal titration calorimetry and fluorescence spectroscopy, which indicated two types of binding sites. Stronger and more specific sites exhibited affinity constants, K1, of 104–106 M–1 and stoichiometries, n1, of 2–13 and dominated at low tannin concentrations. Weaker and less-specific binding sites had K2 constants of 103–105 M–1 ...

  20. Cheddar cheese ripening affects plasma nonesterified fatty acids and serum insulin concentrations in growing pigs

    DEFF Research Database (Denmark)

    Thorning, Tanja Kongerslev; Bendsen, Nathalie T; Jensen, Søren Krogh;

    2015-01-01

    = 0.044 and P = 0.001). Serum insulin was lower in the 24-MRC diet group (1.04 ± 0.09 mmol/L) than in the 4-MRC diet group (1.44 ± 0.14 mmol/L; P = 0.002), but intermediate and not different from either in the 14-MRC diet group (1.25 ± 0.11 mmol/L). Likewise, homeostasis model assessment of insulin...

  1. Dietary vitamin E affects lipid oxidation and total volatiles of irradiated raw turkey meat

    International Nuclear Information System (INIS)

    Breast and leg meat patties, prepared from turkeys fed diets containing 25, 200, 400 or 600 IU of dl-alpha-tocopheryl acetate (TA) per kg diet, were irradiated at 0 or 2.5 kGy with vacuum or loose packaging. The effects of dietary TA on storage stability and production of volatiles in irradiated raw turkey meat were determined. Dietary TA at 200 IU/kg decreased lipid oxidation and reduced total volatiles of raw turkey patties after 7-days of storage. However, the antioxidant effects of dietary TA were more notable when the patties were loosely packaged than when vacuum-packaged. Irradiation increased lipid oxidation of raw turkey meats only when loosely packaged but had limited effects on formation of total volatiles after storage at 4 degrees C for 7 days or longer

  2. The hydroperoxide moiety of aliphatic lipid hydroperoxides is not affected by hypochlorous acid

    OpenAIRE

    Zschaler, Josefin; Arnhold, Jürgen

    2015-01-01

    The oxidation of polyunsaturated fatty acids to the corresponding hydroperoxide by plant and animal lipoxygenases is an important step for the generation of bioactive lipid mediators. Thereby fatty acid hydroperoxide represent a common intermediate, also in human innate immune cells, like neutrophil granulocytes. In these cells a further key component is the heme protein myeloperoxidase producing HOCl as a reactive oxidant. On the basis of different investigation a reaction of the fatty ac...

  3. Affected by abundant PLTP : the atherogenic role of a lipid transfer protein in transgenic mice

    OpenAIRE

    Samyn, Hannelore; Moerland, Matthijs

    2009-01-01

    markdownabstract__Abstract__ Atherosclerosis is a progressive disease of the large and medium-sized arteries. The disease is characterised by endothelial dysfunction, inflammation and the accumulation of fatty and fibrous substances in the vessel wall, resulting in thickening and loss of elasticity of the arteries. The word atherosclerosis has been derived from the Greek words "athera", porridge or gruel, and "skleros", hard or stiff. These words describe the external features of the lipid-lo...

  4. Efficacy of vitamins a and or E as antioxidants against serum glucose, liver glycogen and lipid discrepancy induced by gamma radiation during the estrus cycle of rats

    International Nuclear Information System (INIS)

    This work is directed to study the role of vitamins A and E treatment solely. or in combination against gamma-radiation effects during pro-estrus stage stage on serum glucose, liver content of glycogen, total and lipid fractions (triglycerides, total cholesterol, HDL-and LDL- cholesterol) in serum measured during the estrus stage of the rat estrus cycle. Animals were divided into six groups: untreated control, injected with sesame oil as a vehicle for vitamins injection (i.p.) whole body gamma-irradiated (6 GY), injected with vitamin A Two hours before irradiation, irradiated then injected after one hour with vitamin E, and injected with vitamins A and E pre- and post irradiation. The results demonstrated that irradiation induced significant elevations in the levels of all the measured parameters except in HDL-cholesterol. BOth vitamins ameliorated the recorded elevations during the estrus stage. The combined treatment with vitamins A and E reflected a potent harmony between protective roles of these two antioxidants pre- and post irradiation respectively, that normalized the levels of the measured parameters. The authors suggest that vitamins A and E treatment could enhance and reinforce the natural endogenous defences (steroids, particulary E2) in the body against gamma irradiation and/or other environmental pollutants causing lipid peroxidation, especially during the period of ovulation. Moreover, it is worthy to consider this treatment as a hypolipidemic agent for patients with obesity, atherosclerosis and coronary artery diseases

  5. The impact of diurnal fasting during Ramadan on the lipid profile, hs-CRP, and serum leptin in stable cardiac patients

    Directory of Open Access Journals (Sweden)

    Khafaji HARH

    2011-12-01

    Full Text Available Hadi Abdul Ridha Hadi Khafaji1, Abdulbari Bener2, Mohammed Osman3, Ajayeb Al Merri4, Jassim Al Suwaidi11Department of Cardiology and Cardiovascular Surgery, 2Department of Medical Statistics and Epidemiology, Hamad General Hospital, Hamad Medical Corporation, Doha, State of Qatar; 3Department of Biochemistry, 4Department of Serology, Hamad Medical Corporation, Doha, State of QatarAim: To study the effect of strict prolonged fasting on lipid profile, serum leptin, and high-sensitivity C-reactive protein (hs-CRP in patients with different stable cardiac illnesses and look for associated new cardiac events and any correlation between entire variables.Methods: A total of 56 patients of different stable cardiac illnesses were followed in our cardiology outpatient for 3 months. Data concerning their ability to fast were collected: New York Heart Association class of congestive cardiac failure, angina class, previous myocardial infarction, previous coronary artery bypass graft, percutaneous coronary intervention, severity of valvular lesion, metallic prosthetic valve, and traditional risk factors (diabetes mellitus, insulin requirement, hypertension, hypercholesterolemia, smoking habit, and obesity. Detailed clinical examination and electrocardiography were performed in all patients in three consecutive visits before, during, and after Ramadan. Echocardiographic and angiographic findings and medication plans were collected from patient records. Lipid profile, serum leptin, and hs-CRP were assessed before, during, and after Ramadan.Results: All patients fasted during Ramadan: 80.4% were male, 67.9% were aged >50 years, 71.4% had no change in their symptoms during fasting while 28.6% felt better. No patient has deteriorated. 91.1% of the patients were compliant with medicine during Ramadan, 73.2% after. 89.3% were compliant with diet during Ramadan with no significant change in body weight in the follow-up period. No cardiac or noncardiac morbidity or

  6. Relationship of serum lipids to adrenal-gland uptake of 6β-[131I] iodomethyl-19-norcholesterol in Cushing's syndrome

    International Nuclear Information System (INIS)

    An alteration in serum cholesterol levels has been suggested as a possible modifier of adrenal uptake of the cholesterol analog, 6β-[131I]iodomethyl-19-norcholesterol (NP-59). To assess the effect of hypercholesterolemia upon NP-59 adrenal uptake, patients with Cushing's syndrome (eight with pituitary-dependent, four with ACTH-independent, and two with ectopic-ACTH syndrome) were selected for retrospective analysis based on the availability of serum cholesterol (n = 14) and triglyceride (n = 10) concentrations obtained at the time of adrenal scintigraphy. A negative correlation (r = -0.78, p < 0.01) was found between NP-59 uptake and serum cholesterol levels in patients with pituitary-dependent Cushing's disease. Compared with pituitary-dependent disease, the ectopic-ACTH syndrome and ACTH-independent states demonstrated equal or greater adrenal uptake of NP-59 at similar serum cholesterol concentrations. Serum triglyceride concentrations did not correlate with total adrenal uptake of NP-59 in any of the patient groups studied. Increased serum cholesterol concentrations are associated with diminished adrenal uptake of NP-59, and in some cases may limit the diagnoic efficacy of adrenal scintigraphy in Cushing's syndrome

  7. Effects of raloxifene hydrochloride on bone mineral density, bone metabolism and serum lipids in postmenopausal women: a randomized clinical trial in Beijing

    Institute of Scientific and Technical Information of China (English)

    ZHENG Shurong 郑淑蓉; Abie Ekangaki; Jodie Stocks; Kristine Harper; LIU Jianli 刘建立; WU Yiyong 吴宜勇; ZHANG Zhonglan 张忠兰; YANG Xin 杨欣; HUI Ying 惠英; ZHANG Ying 张颖; CHEN Shuling 陈淑玲; DENG Wenhui 邓文慧; LIU Hui 刘慧

    2003-01-01

    Objective To determine the effects of raloxifene hydrochloride (RLX) on bone mineral density (BMD), bone metabolism markers and serum lipids in healthy postmenopausal women in Beijing.Methods A multicenter, randomized, double-blind, placebo-controlled study was conducted in a total of 204 healthy postmenopausal women (age 59.5±5.0 years and weight 62.8±8.7 kg) treated with either RLX 60 mg (n=102) or placebo (n=102) daily for 12 months. BMD, serum lipids, and bone markers were measured before and after drug administration.Results Compared with placebo, RLX produced a significant increase in both total lumbar spine and total hip BMD. For the lumbar spine, percentage increase in total BMD was 2.3% with RLX compared with a decrease of 0.1% with placebo (P<0.001). Corresponding values for total hip BMD were a 2.5% increase for RLX and a 1.1% increase for placebo (P=0.011). For biochemical markers of bone metabolism, serum osteocalcin and C-telopeptide, percentage decreases were 27.65% and 24.02% in RLX-treated subjects. Corresponding values in placebo were a 10.64% decrease and a 15.75% increase (RLX compared with placebo, both P<0.001). For total cholesterol and low-density lipoprotein cholesterol levels, percentage decreases were 6.44% and 34.58% in the RLX-treated group. Corresponding values in placebo-treated patients were a 1.44% increase and a 19.07% decrease (RLX compared with placebo, both P<0.001). No differences were found for high-density lipoprotein cholesterol or triglyceride levels between the two groups. Only 5 subjects discontinued early owing to an adverse event (3 in the RLX group and 2 in the placebo group). Conclusions This study confirms that RLX exerts positive effects on the skeleton, increasing BMD and decreasing biochemical markers of bone metabolism, and has a positive effect on the overall serum lipid profile in postmenopausal women in China.

  8. Is the fact of parenting couples cohabitation affecting the serum levels of persistent organohalogen pollutants?

    Science.gov (United States)

    Góralczyk, Katarzyna; Struciński, Paweł; Wojtyniak, Bogdan; Rabczenko, Daniel; Lindh, Christian H; Jönsson, Bo A G; Toft, Gunnar; Lenters, Virissa; Czaja, Katarzyna; Hernik, Agnieszka; Bonde, Jens Peter; Pedersen, Henning S; Zvyezday, Valentyna; Ludwicki, Jan K

    2015-06-01

    Organohalogen compounds constitute one of the important groups of persistent organic pollutants (POPs). Among them, due to their long-term health effects, one should pay attention on organochlorine pesticides, polychlorinated biphenyls (PCBs) and perfluoroalkylated substances (PFASs). This paper is an attempt to answer the question about relation between the fact of cohabitation by couples expecting a child and the level of the organohalogen compounds in the blood serum of both parents. The study was done on a population of parent couples from Greenland, Poland and Ukraine, from whom blood samples were collected in order to establish the levels of marker organohalogen compounds. We selected, as the representative of these compounds, the most persistent metabolite of DDT, i.e. p,p'-DDE, the most frequently detected PCB congener - CB-153, and PFOS and PFOA as the representatives of PFASs. The results show that in case of all compounds under study the highest concentrations were present always in men in relation to the levels detected in the blood serum of their female partners, regardless of the country of origin of the couple. A positive correlation was noted between the concentrations of the studied compounds in the blood serum of men and women in parenting couples. In some cases these correlations were statistically significant, e.g. for concentrations of p,p'-DDE in pairs from Greenland and Ukraine, of CB-153 in pairs from Poland and Ukraine, and of PFOS for parents from Greenland and Poland, while for PFOA - only for couples from Greenland. The concentrations of the compounds included in the study were similar to the levels found in general population in other countries. Our results show that the exposure to POPs resulting from cohabitation plays a role in the general exposure to these compounds. PMID:25836759

  9. Immunofluorescence in multiple tissues utilizing serum from a patient affected by systemic lupus erythematosus

    OpenAIRE

    Piotr Brzezinski; Howard, Michael S.; Ana Maria Abreu Velez

    2012-01-01

    Introduction: Lupus erythematosus is a chronic, inflammatory autoimmune disease that can affect multiple organs. Lupus can affect many parts of the body, especially in systemic lupus erythematosus (SLE); affected tissues may include the joints, skin, kidneys, heart, lungs, blood vessels, and brain. Case report: A 46-year-old female presented with pruritus, photosensitivity and edema of the cheeks of about 2 years duration, and was evaluated by a dermatologist. On examination, multiple telangi...

  10. Long-Term Supplementation with Beta Serum Concentrate (BSC, a Complex of Milk Lipids, during Post-Natal Brain Development Improves Memory in Rats

    Directory of Open Access Journals (Sweden)

    Jian Guan

    2015-06-01

    Full Text Available We have previously reported that the supplementation of ganglioside-enriched complex-milk-lipids improves cognitive function and that a phospholipid-enriched complex-milk-lipid prevents age-related cognitive decline in rats. This current study evaluated the effects of post-natal supplementation of ganglioside- and phospholipid-enriched complex-milk-lipids beta serum concentrate (BSC on cognitive function in young rats. The diet of male rats was supplemented with either gels formulated BSC (n = 16 or blank gels (n = 16 from post-natal day 10 to day 70. Memory and anxiety-like behaviors were evaluated using the Morris water maze, dark–light boxes, and elevated plus maze tests. Neuroplasticity and white matter were measured using immunohistochemical staining. The overall performance in seven-day acquisition trials was similar between the groups. Compared with the control group, BSC supplementation reduced the latency to the platform during day one of the acquisition tests. Supplementation improved memory by showing reduced latency and improved path efficiency to the platform quadrant, and smaller initial heading error from the platform zone. Supplemented rats showed an increase in striatal dopamine terminals and hippocampal glutamate receptors. Thus BSC supplementation during post-natal brain development improved learning and memory, independent from anxiety. The moderately enhanced neuroplasticity in dopamine and glutamate may be biological changes underlying the improved cognitive function.

  11. Effect of soybean seeds alone or in combination with insulin or glibenclamide on serum lipid profiles in alloxan-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    O. H. Aziz

    2009-01-01

    Full Text Available The present study was conducted to evaluate the effect of soybean seeds administration alone or combined with either insulin or glibenclamide on serum lipid profiles in diabetic rats. Male Wister rats were induced diabetes by a single subcutaneous injection of alloxan 100 mg/kg.b.w.The rats randomly divided into six groups (eight rats in each group: The first group served as a control, the second group was administered soybean seed 400 mg/kg.b.w orally as suspension, the third group injected insulin 10 I.U/kg.b.w, subcutaneously, the fifth group administered glibenclamide 5 mg/kg.b.w. orally. Accompaniment of soybean seeds with either of insulin or glibenclamide given the fourth and sixth groups, using the same routes and doses in the individual groups. All treatments were once daily for two weeks. Soybean seeds treatment alone resulted in an improvement of body weight and decrease of triglyceride and total lipids levels. While treatment with insulin or glibenclamide as alone or combination with soybean seeds showed a significant reduction in the levels of total cholesterol, triglyceride, low density lipoprotein, and total lipids, beside a significant increase in both body weight and high density lipoprotein. Beneficial effects were seen when soybean seeds combined with either of insulin or glibenclamide treatment. These results indicate the usefulness of soybean seeds in the management of diabetes through the hypolipidemic effects of soybean seeds in diabetic rats.

  12. Comparison of the Effects of Edible Oils: Rice Bran, Grape Seed, and Canola on Serum Lipid Profile and Paraoxonase Activity in Hyperlipidemic Rats

    Directory of Open Access Journals (Sweden)

    Maryam Ranjbar-Zahedani

    2015-03-01

    Full Text Available Background: Dyslipidemia is considered as one of the crucial contributors to cardio- cerebro-vascular diseases. Objectives: The present study aimed to compare the effects of Rice Barn Oil (RBO, Grape Seed Oil (GSO, and Canola Oil (CO on dyslipidemia and oxidative stress in experimentally induced hyperlipidemic rats. Materials and Methods: In the present experimental study, forty hyperlipidemic male Wistar rats were randomly assigned to 4 groups to receive RBO, GSO, or CO or Soy Bean Oil (SBO, as controls, for 4 weeks following a 3-week period of Atherogenic Diet (AD intake. Blood samples were collected at the beginning of the study, after inducing dyslipidemia, and at the end of the experimental period. Then, the data were entered into the SPSS statistical software (v. 13.0 and analyzed using paired t-test, paired sample Wilcoxon signed rank test, and Kruskal-Wallis test. Results: AD elevated lipid and/or lipoprotein profile and decreased the paraoxonase activity in the hyperlipidemic rats. The results of paired t-test revealed that RBO led to a significant improvement in serum lipoprotein profile and paraoxonase activity. Besides, a significant difference was found in the GSO group regarding all the measured parameters, except for paraoxonase activity. Moreover, CO diet showed a significant hypolipidemic effect on serum Triglyceride (TG and Total Cholesterol (TC and led to a slight improvement in Low Density Lipoprotein-Cholesterol (LDL-C and High Density Lipoprotein-Cholesterol (HDL-C. Conclusions: The results of the present study suggested that vegetable oils, including RBO, GSO, and CO, might improve dyslipidemia and oxidative stress in hyperlipidemic rats. Indeed, substituting saturated fatty acids with unsaturated fatty acids in rats’ diet had beneficial effects on serum lipid profile and oxidative stress. Comparison of the 3 edible oils showed that GSO had a more profound effect on decreasing hyperlipidemia.

  13. Assessment risk of osteoporosis in Chinese people: relationship among body mass index, serum lipid profiles, blood glucose, and bone mineral density

    Science.gov (United States)

    Cui, Rongtao; Zhou, Lin; Li, Zuohong; Li, Qing; Qi, Zhiming; Zhang, Junyong

    2016-01-01

    Objective The aim of our study was to investigate the relationship among age, sex, body mass index (BMI), serum lipid profiles, blood glucose (BG), and bone mineral density (BMD), making an assessment of the risk of osteoporosis. Materials and methods A total of 1,035 male and 3,953 female healthy volunteers (aged 41–95 years) were recruited by an open invitation. The basic information, including age, sex, height, weight, waistline, hipline, menstrual cycle, and medical history, were collected by a questionnaire survey and physical examination. Serum lipid profiles, BG, postprandial blood glucose, and glycosylated hemoglobin were obtained after 12 hours fasting. BMD in lumbar spine was measured by dual-energy X-ray absorptiometry scanning. Results The age-adjusted BMD in females was significantly lower than in males. With aging, greater differences of BMD distribution exist in elderly females than in males (P7.0 mmol/L was lower than in people with BG of ≤7.0 mmol/L (P<0.001). People with serum high-density lipoprotein cholesterol levels of ≥1.56 mmol/L had a greater prevalence of osteoporosis compared with high-density lipoprotein cholesterol ≤1.55 mmol/L. Logistic regression with odds ratios showed that no association was found among total cholesterol, triglyceride, low-density lipoprotein cholesterol, glycosylated hemoglobin, postprandial blood glucose and BMD. Conclusion The present study further confirmed that factors such as age, sex, weight, BMI, high-density lipoprotein cholesterol, and diabetes are significant predictors of osteoporosis in the Chinese people. PMID:27445467

  14. Cocoa and Whey Protein Differentially Affect Markers of Lipid and Glucose Metabolism and Satiety.

    Science.gov (United States)

    Campbell, Caroline L; Foegeding, E Allen; Harris, G Keith

    2016-03-01

    Food formulation with bioactive ingredients is a potential strategy to promote satiety and weight management. Whey proteins are high in leucine and are shown to decrease hunger ratings and increase satiety hormone levels; cocoa polyphenolics moderate glucose levels and slow digestion. This study examined the effects of cocoa and whey proteins on lipid and glucose metabolism and satiety in vitro and in a clinical trial. In vitro, 3T3-L1 preadipocytes were treated with 0.5-100 μg/mL cocoa polyphenolic extract (CPE) and/or 1-15 mM leucine (Leu) and assayed for lipid accumulation and leptin production. In vivo, a 6-week clinical trial consisted of nine panelists (age: 22.6 ± 1.7; BMI: 22.3 ± 2.1) consuming chocolate-protein beverages once per week, including placebo, whey protein isolate (WPI), low polyphenolic cocoa (LP), high polyphenolic cocoa (HP), LP-WPI, and HP-WPI. Measurements included blood glucose and adiponectin levels, and hunger ratings at baseline and 0.5-4.0 h following beverage consumption. At levels of 50 and 100 μg/mL, CPE significantly inhibited preadipocyte lipid accumulation by 35% and 50%, respectively, and by 22% and 36% when combined with 15 mM Leu. Leu treatment increased adipocyte leptin production by 26-37%. In the clinical trial, all beverages significantly moderated blood glucose levels 30 min postconsumption. WPI beverages elicited lowest peak glucose levels and HP levels were significantly lower than LP. The WPI and HP beverage treatments significantly increased adiponectin levels, but elicited no significant changes in hunger ratings. These trends suggest that combinations of WPI and cocoa polyphenols may improve markers of metabolic syndrome and satiety. PMID:26987021

  15.  The Association Between Body Mass Index, Lipid Profile and Serum Estradiol Levels in a Sample of Iraqi Diabetic Premenopausal Women

    Directory of Open Access Journals (Sweden)

    Mahmood Shakir Al-Zaidi

    2011-07-01

    Full Text Available  Objectives: To evaluate the association between body mass index (BMI, serum lipid profile and estradiol level in a sample of Iraqi diabetic premenopausal women at their pre ovulatory period.Methods: A total of 155 diabetic female patients aged between 20-45 years, were enrolled in this study, which was conducted in Al Khademiyia Teaching Hospital from July 2008 to January 2010. Venous blood samples were taken, each serum sample was analyzed for total cholesterol, triglycerides, high density lipoprotein - cholesterol (HDL-C, low density lipoprotein - cholesterol (LDL-C, fasting glucose, insulin and estradiol. Body weight and height were measured for all patients included to calculate their Body Mass Index.Results: The age of diabetic patients was 29.7 ± 4.3 years and BMI was 28.3 ±5.84 kg/m2. Fifty patients had normal BMI, while 50 patients were overweight and 55 were obese. The study revealed a positive association between obesity, triglycerides and LDL-C and a negative association between serum estradiol, obesity and LDL-C.Conclusion: Iraqi diabetic pre menopausal women at their pre ovulatory period exhibit a pro-atherogenic risk profile because of their abnormal BMI, higher LDL-C, lower HDL-cholesterol and estradiol level.

  16. FADS gene polymorphisms in Koreans: association with _6 polyunsaturated fatty acids in serum phospholipids, lipid peroxides, and coronary artery disease

    Science.gov (United States)

    Cardiovascular diseases are multifactorial and blood lipids are one of their best characterized risk factors. In addition to blood cholesterol levels, triglycerides (TG) are also important risk factors and their levels are determined by genetic and environmental factors such as diet. Regarding the g...

  17. Effects of dietary polyunsaturated fatty acids on myocardial and serum lipids in normoxic and chronically hypoxic rat heart

    Czech Academy of Sciences Publication Activity Database

    Balková, P.; Ježková, J.; Hlaváčková, M.; Neckář, Jan; Staňková, B.; Kolář, František; Novák, F.; Nováková, O.

    Praha: IV. interní klinika 1. LF UK Praha, 2008, s. 1-5. ISBN 978-80-254-2834-4. [Atherosklerosa 2008. Praha (CZ), 10.09.2008-12.09.2008] Institutional research plan: CEZ:AV0Z50110509 Keywords : heart * chronic hypoxia * lipid diet Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery

  18. The ACTN3 R577X polymorphism affects the lipid profile and the prognosis of nutritional intervention in HIV-positive patients.

    Science.gov (United States)

    Aranalde, Laura C R; Pederzoli, Bruna S; Marten, Thais; Barros, Flavio C; Basso, Rossana P; Silveira, Jussara M; Valle, Sandra C; Pieniz, Simone; Araujo, Ronaldo C; Paredes-Gamero, Edgar J; Schadock, Ines; Schneider, Augusto; Barros, Carlos C

    2016-06-01

    The hypothesis of the present study is that the polymorphisms in the APOC3, CEPT, ACE, and ACTN3 genes can affect the outcome of nutritional intervention and the plasma lipid profile of HIV+ patients. To test the hypothesis, genetic material was collected from buccal cells, and serum was collected for biochemical analysis. Sixty-five patients were analyzed. The incorporation of protease inhibitor (PI) was more frequent in women (77% vs 33% in men). Nutritional intervention improved anthropometric parameters independent of the genotype. Patients with the RR genotype for the ACTN3 R577X polymorphism had lower glycemia (RR = 95.4 ± 6.5 mg/dL, RX = 102.6 ± 10.6 mg/dL, XX = 110.1 ± 16.3 mg/dL; P = .03) and a greater reduction in low-density lipoproteins (LDL) after intervention (LDL: RR = -23.7 ± 15.8 mg/dL, RX = 1.32 ± 5.13 mg/dL, XX = 30.21 ± 24.4 mg/dL; P = .01). Patients using PI had a negative response to dietary intervention regarding the levels of high-density lipoprotein (-2.4 ± 1.70 with PI, 2.56 ± 1.60 mg/dL without PI; P = .02), very low density lipoprotein (0.84 ± 2.73 with IP, -5.46 ± 3.37 mg/dL without PI; P = .03), and triglycerides (1.79 ± 13.22 with PI, -34.00 ± 17.67 mg/dL without PI; P = .052). This response was also independent of the genotype (P > 0.05) and suggested the need for oral lipid-lowering drugs in all HIV+ patients using PI. Our results indicate that the ACTN3 R577X polymorphism is a good predictor of both the lipid profile and the prognosis of nutritional intervention in reducing LDL in HIV+ patients. PMID:27188902

  19. A lignan complex isolated from flaxseed does not affect plasma lipid concentrations or antioxidant capacity in healthy postmenopausal women

    DEFF Research Database (Denmark)

    Hallund, Jesper; Ravn-Haren, Gitte; Bügel, S.; Tholstrup, T.; Tetens, Inge

    2006-01-01

    ) completed a randomized, double-blind, placebo-controlled, crossover study. Women consumed daily a low-fat muffin, with or without a lignan complex, for 6 wk, separated by a 6-wk washout period. Serum ENL concentration, urinary ENL excretion, plasma concentrations of total cholesterol (TC), LDL cholesterol.......001) and ENL urinary excretion (P <0.001) were significantly higher after the lignan complex intervention period compared with placebo. Plasma concentrations of TC, LDL-C, HDL-C, TAG, lipoprotein oxidation lag time, TEAC and FRAP were not affected. Daily consumption for 6 wk of a low-fat muffin enriched...

  20. Effects of raloxifene hydrochloride on bone mineral density, bone metabolism and serum lipids in Chinese postmenopausal women with osteoporosis:a multi-center, randomized, placebo-controlled clinical trial

    Institute of Scientific and Technical Information of China (English)

    LIU Jian-li刘建立; LIU Hui刘慧; CHEN Xiao-ping陈小平; LIU Yu-juan刘玉娟; Abie Ekangaki; ZHENG Yi-man郑以漫; Adolfo Diez-Perez; Kristine Harper; ZHU Han-min朱汉民; HUANG Qi-ren黄琪仁; ZHANG Zhong-lan张忠兰; LI Hui-lin李慧林; QIN Yue-juan秦跃娟; ZHANG Ying张颖; WEI Dao-lin魏道林; LU Jing-hui陆敬辉

    2004-01-01

    Background Raloxifene has been approved for prevention and treatment of postmenopausal osteoporosis in Caucasian women. It also has some positive effects on serum lipids in Caucasians. The objective of this study was to determine the effect of raloxifene hydrochloride on lumbar spine and total hip bone mineral density (BMD), bone metabolism, and serum lipids in Chinese postmenopausal women with osteoporosis.Methods This was a multi-center, randomized, double-blind, placebo-controlled clinical trial in which 204 postmenopausal Chinese women with osteoporosis were assigned to receive raloxifene (60 mg) or placebo treatment daily for 12 months. BMD, serum bone metabolism markers, and serum lipids were measured before and after drug administration. BMD was measured by Dual-Energy X-Ray Absorptiometry (DEXA) and bone metabolism markers were analyzed by one-step enzyme-linked immunosorbent assay. Serum lipids were measured by enzymatic analysis.Results At the end of the 12-month study, lumbar spine BMD increased in both groups with a mean increase of (3.3±4.8) % in the raloxifene group and (1.0±4.9) % in the placebo group (P0.05). In the raloxifene group, the median decreases in the biochemical markers of bone metabolism serum osteocalcin and C-telopeptide were 41.7% and 61.5%, respectively. These changes were statistically significant compared with those in the placebo group (10.6% and 35.6%, P<0.001, respectively). Both total cholesterol and low-density lipoprotein cholesterol decreased significantly in the raloxifene group compared with those in the placebo group (P<0.001, respectively) and there was no significant effect of raloxifene on high-density lipoprotein cholesterol and triglycerides compared with placebo. Conclusions Raloxifene 60 mg/d for 12 months significantly increases lumbar spine and total hip BMD, significantly decreases bone turnover, and has favourable effects on serum lipids in Chinese postmenopausal women with osteoporosis.

  1. Chronic exposure to sublethal hexavalent chromium affects organ histopathology and serum cortisol profile of a teleost, Channa punctatus (Bloch)

    International Nuclear Information System (INIS)

    Effects of chronic exposures (one and two months) to sublethal doses of hexavalent chromium (2 and 4 mg/L potassium dichromate) on organ histopathology and serum cortisol profile were investigated and their overall impact on growth and behavior of a teleost fish, Channa punctatus was elucidated. Histopathological lesions were distinct in the vital organs gill, kidney and liver. The gill lamellae became lifted, fused, and showed oedema. Hyperplasia and hypertrophy of lamellar epithelial cells were distinct with desquamation. Hypertrophy of epithelial cells of renal tubules and reduction in tubular lumens were observed in the trunk kidney. The atrophy of the head kidney interrenal cells and decreased serum cortisol level indicated exhaustion of interrenal activity. Hepatocyte vacuolization and shrinkage, nuclear pyknosis and increase of sinusoidal spaces were observed in the liver. Abnormal behavioral patterns and reduced growth rate were also noticed in the exposed fish. The chronic hexavalent chromium exposure thus by affecting histopathology of gill, kidney (including interrenal tissue) and liver could impair the vital functions of respiration, excretion, metabolic regulation and maintenance of stress homeostasis which in the long-run may pose serious threat to fish health and affect their population.

  2. Estrogen receptor alpha gene polymorphism associated with type 2 diabetes mellitus and the serum lipid concentration in Chinese women in Guangzhou

    Institute of Scientific and Technical Information of China (English)

    HUANG Qin; WANG Ting-huai; LU Wen-sheng; MU Pan-wei; YANG Yan-fang; LIANG Wei-wen; LI Cai-xia; LIN Gui-ping

    2006-01-01

    Background Estrogen might play an important role in type 2 diabetes mellitus pathogenesis. A number of polymorphisms have been reported in the estrogen receptor alpha (ERα) gene (also named ESR1), including the XbaI and PvuⅡ restriction enzyme polymorphisms of ESR1, which may be involved in disease pathogenesis. The aim of this study was to determine whether ERα gene polymorphisms are associated with type 2 diabetes mellitus and serum lipid level.Methods Two hundred and ninety-nine patients with type 2 diabetes mellitus were compared with three hundred and forty-one health controls of Guangzhou in China, both were male and postmenopausal female residents at 51-70 years. ESR1 genotyping was performed using polymerase chain reaction (PCR) and PvuⅡ and XbaI restriction fragment length polymorphism (PCR-RFLP) analysis.Results ESR1 allelic frequencies of P, p and X, x alleles were 0.408, 0.592; 0.360, 0.640 in the type 2 diabetes mellitus group and 0.318, 0.682; 0.328, 0.672 in the control group, respectively. In case-control study, there was significant difference in PvuⅡ, but not XbaI, allele frequency between the type 2 diabetes mellitus and control groups (P=0.001 and P=0.122). When the group was separated into men and women, the difference was significant in women (P<0.001) but not in men (P=0.854) with the PvuⅡ genotype, and the effect of PvuⅡ variant on the development of type 2 diabetes mellitus was improved with aging. In addition, PvuⅡ genotype was associated with blood glucose [fasting blood glucose (FBG), postprandial blood glucose (PBG)] and serum lipid [total cholesterol (TC) and low density lipoprotein (LDL)-c] concentration in healthy women.Conclusions PvuⅡ polymorphism of ESR1 increases susceptibility to type 2 diabetes mellitus in Chinese Guangzhou women. ESR1 variants may also impact serum lipid metabolism, which might provide a mechanism connecting ESR1 to type 2 diabetes.

  3. Dietary conjugated linoleic acids affect tissue lipid composition but not de novo lipogenesis in finishing pigs

    OpenAIRE

    Bee, Giuseppe

    2001-01-01

    Influence de l'apport des acides linoléiques conjugués dans l'alimentation sur la composition des tissus adipeux et la lipogenèse de novo chez les porcs en finition. Plusieurs travaux ont révélé que les acides linoléiques conjugués (conjugated linoleic acids : CLA) ont une influence importante sur le métabolisme des lipides et des protéines. En revanche, les effets des CLA sur le profil des acides gras dans les tissus adipeux chez le porc sont peu connus. Dans cette étude, nous avons détermin...

  4. Effects of chicory inulin on serum metabolites of uric acid, lipids, glucose, and abdominal fat deposition in quails induced by purine-rich diets.

    Science.gov (United States)

    Lin, Zhijian; Zhang, Bing; Liu, Xiaoqing; Jin, Rui; Zhu, Wenjing

    2014-11-01

    Inulin, a group of dietary fibers, is reported to improve the metabolic disorders. In the present study, we investigated the effects of chicory inulin on serum metabolites of uric acid (UA), lipids, glucose, and abdominal fat deposition in quail model induced by a purine-rich diet. In this study, 60 male French quails were randomly allocated to five groups: CON (control group), MOD (model group), BEN (benzbromarone-treated group), CHI-H (high-dosage chicory inulin-treated group), and CHI-L (low-dosage chicory inulin-treated group). The serum UA level was significantly increased in the model group from days 7 to 28, as well as triglyceride (TG) and free fatty acid (FFA) increased later in the experimental period. The abdominal fat ratio was increased on day 28. Benzbromarone can decrease UA levels on days 14 and 28. The high and low dosage of chicory inulin also decreased serum UA levels on days 7, 14, and 28. The abdominal fat ratio, activity, and protein of acetyl-CoA carboxylase (ACC) were decreased in chicory inulin-treated groups. The activities of xanthine oxidase (XOD) and fatty acid synthase (FAS) were increased in the model group and decreased in the benzbromarone and chicory inulin groups. This study evaluated a quail model of induced hyperuricemia with other metabolic disorders caused by a high-purine diet. The results indicated that a purine-rich diet might contribute to the development of hyperuricemia, hypertriglyceridemia, and abdominal obesity. Chicory inulin decreased serum UA, TG, and abdominal fat deposition in a quail model of hyperuricemia by altering the ACC protein expression and FAS and XOD activities. PMID:25314375

  5. A review of nutritional and physiological factors affecting goat milk lipid synthesis and lipolysis.

    Science.gov (United States)

    Chilliard, Y; Ferlay, A; Rouel, J; Lamberet, G

    2003-05-01

    Although the effect of lactation stage is similar, the responses of milk yield and composition (fat and protein contents) to different types of lipid supplements differ greatly between goats and cows. Milk fat content increases with almost all studied fat supplements in goats but not in cows. However, the response of milk fatty acid (FA) composition is similar, at least for major FA, including conjugated linoleic acid (CLA) in goats and cows supplemented with either protected or unprotected lipid supplements. Goat milk CLA content increases sharply after either vegetable oil supplementation or fresh grass feeding, but does not change markedly when goats receive whole untreated oilseeds. Important interactions are observed between the nature of forages and of oil supplements on trans-10 and trans-11 C18:1 and CLA. Peculiarities of goat milk FA composition and lipolytic system play an important role in the development of either goat flavor (release of branched, medium-chain FA) or rancidity (excessive release of butyric acid). The lipoprotein lipase (LPL) activity, although lower in goat than in cow milk, is more bound to the fat globules and better correlated to spontaneous lipolysis in goat milk. The regulation of spontaneous lipolysis differs widely between goats and cows. Goat milk lipolysis and LPL activity vary considerably and in parallel across goat breeds or genotypes, and are low during early and late lactation, as well as when animals are underfed or receive a diet supplemented with protected or unprotected vegetable oils. This could contribute to decreases in the specific flavor of goat dairy products with diets rich in fat. PMID:12778586

  6. Serum bile acids are higher in humans with prior gastric bypass: potential contribution to improved glucose and lipid metabolism

    DEFF Research Database (Denmark)

    Patti, Mary-Elizabeth; Houten, Sander M; Bianco, Antonio C;

    2009-01-01

    index cohort (n = 10). Total serum bile acid concentrations were higher in GB (8.90 +/- 4.84 micromol/l) than in both overweight (3.59 +/- 1.95, P = 0.005, Ov) and severely obese (3.86 +/- 1.51, P = 0.045, MOb). Bile acid subfractions taurochenodeoxycholic, taurodeoxycholic, glycocholic...

  7. Study of the Effect of Garlic on Serum Lipids and Blood Glucose Levels in Type 2 Diabetic Patients

    OpenAIRE

    M Afkhami - Ardekani; AR Kamali - Ardakani

    2005-01-01

    Introduction: Hyperlipidemia and diabetes are common risk factors for ischemic heart disease, which is the main cause of mortality in diabetic patients. Strict control of blood glucose and other risk factors in diabetics has led to prevention of complications. Garlic has received particular attention for control of blood glucose and decrease in blood lipid levels. At present, several studies have been carried out in order to prove advantages of garlic. Methods: In this study, effects of garsi...

  8. Size and molecular flexibility affect the binding of ellagitannins to bovine serum albumin.

    Science.gov (United States)

    Dobreva, Marina A; Green, Rebecca J; Mueller-Harvey, Irene; Salminen, Juha-Pekka; Howlin, Brendan J; Frazier, Richard A

    2014-09-17

    Binding to bovine serum albumin of monomeric (vescalagin and pedunculagin) and dimeric ellagitannins (roburin A, oenothein B, and gemin A) was investigated by isothermal titration calorimetry and fluorescence spectroscopy, which indicated two types of binding sites. Stronger and more specific sites exhibited affinity constants, K1, of 10(4)-10(6) M(-1) and stoichiometries, n1, of 2-13 and dominated at low tannin concentrations. Weaker and less-specific binding sites had K2 constants of 10(3)-10(5) M(-1) and stoichiometries, n2, of 16-30 and dominated at higher tannin concentrations. Binding to stronger sites appeared to be dependent on tannin flexibility and the presence of free galloyl groups. Positive entropies for all but gemin A indicated that hydrophobic interactions dominated during complexation. This was supported by an exponential relationship between the affinity, K1, and the modeled hydrophobic accessible surface area and by a linear relationship between K1 and the Stern-Volmer quenching constant, K(SV). PMID:25162485

  9. Lactic acid bacteria affect serum cholesterol levels, harmful fecal enzyme activity, and fecal water content

    Directory of Open Access Journals (Sweden)

    Chung Myung

    2009-06-01

    Full Text Available Abstract Background Lactic acid bacteria (LAB are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as lower cholesterol. Although present in many foods, most trials have been in spreads or dairy products. Here we tested whether Bifidobacteria isolates could lower cholesterol, inhibit harmful enzyme activities, and control fecal water content. Methods In vitro culture experiments were performed to evaluate the ability of Bifidobacterium spp. isolated from healthy Koreans (20~30 years old to reduce cholesterol-levels in MRS broth containing polyoxyethanylcholesterol sebacate. Animal experiments were performed to investigate the effects on lowering cholesterol, inhibiting harmful enzyme activities, and controlling fecal water content. For animal studies, 0.2 ml of the selected strain cultures (108~109 CFU/ml were orally administered to SD rats (fed a high-cholesterol diet every day for 2 weeks. Results B. longum SPM1207 reduced serum total cholesterol and LDL levels significantly (p B. longum SPM1207 also increased fecal LAB levels and fecal water content, and reduced body weight and harmful intestinal enzyme activities. Conclusion Daily consumption of B. longum SPM1207 can help in managing mild to moderate hypercholesterolemia, with potential to improve human health by helping to prevent colon cancer and constipation.

  10. The Effect of a Monthly Injectable Contraceptive Norethisterone plus Estradiol Valerate on Serum Lipids in Chinese Women

    Institute of Scientific and Technical Information of China (English)

    杨华; 张美云; 卢凤英; 蒋海英

    1993-01-01

    The effect of an once-a-month injectable contraceptive ( Norethisterone enanthate 50mg plas estradiol valerate 5mg ) on lipids metabolism was evaluated in 22 Chinese women. Other 20 women served as control group. Fasting blood samples were taken at the pre-treatment cycle, the cycles afier first, third, sixth and twelfth month during one year treatment and one post-treatment cycle. The changes of the total cholesterol, total triglycerides, HDL-cholesterol and LDL-cholesterol and the ratio of HDL-C / LDL-C were observed. The results showed that the total cholesterol at the 1st and 3rd month of treatment was significantly decreased (P< 0.01) as comparedwith that of pretreatment value and control group. The HDL-cholesterol and totaltriglycerides were also significantly decreased ( P< 0.05) from the 1st to 12th treatment cycle. After discontinuation, total cholesterol, total triglycerides and HDL-cholesterol recovered to the pretreatment levels. The LDL-cholesterol remained unchanged during the whole course. This study suggests that the monthiy injectable contraceptive induces the change in lipid metabolism without significant changes for HDL-C / LDL-C ratio. Although the lipids level occurred to change in the treatment cycle, it recovered quickly at discontinuation of injection. These alterations may be contributed to the functionof body regulation. It can be concluded that there is no obvious relationship between NET-OEN plus EV and CVD.

  11. The effect of Gongronema latifolium extracts on serum lipid profile and oxidative stress in hepatocytes of diabetic rats

    Indian Academy of Sciences (India)

    N H Ugochukwu; N E Babady; M Cobourne; S R Gasset

    2003-02-01

    Diabetes is known to involve oxidative stress and changes in lipid metabolism. Many secondary plant metabolites have been shown to possess antioxidant activities, improving the effects of oxidative stress on diabetes. This study evaluated the effects of extracts from Gongronema latifolium leaves on antioxidant enzymes and lipid profile in a rat model of non insulin dependent diabetes mellitus (NIDDM). The results confirmed that the untreated diabetic rats were subjected to oxidative stress as indicated by significantly abnormal activities of their scavenging enzymes (low superoxide dismutase and glutathione peroxide activities), compared to treated diabetic rats, and in the extent of lipid peroxidation (high malondialdehyde levels) present in the hepatocytes. The ethanolic extract of G. latifolium leaves possessed antioxidant activity as shown by increased superoxide dismutase and glutathione peroxidase activities and decreases in malondialdehyde levels. High levels of triglycerides and total cholesterol, which are typical of the diabetic condition, were also found in our rat models of diabetes. The ethanolic extract also significantly decreased triglyceride levels and normalized total cholesterol concentration.

  12. Glycyrrhizic acid improved lipoprotein lipase expression, insulin sensitivity, serum lipid and lipid deposition in high-fat diet-induced obese rats

    Directory of Open Access Journals (Sweden)

    Eu Chia

    2010-07-01

    Full Text Available Abstract Background The metabolic syndrome, known also as the insulin resistance syndrome, refers to the clustering of several risk factors for atherosclerotic cardiovascular disease. Dyslipidaemia is a hallmark of the syndrome and is associated with a whole body reduction in the activity of lipoprotein lipase (LPL, an enzyme under the regulation of the class of nuclear receptors known as peroxisome proliferator-activated receptor (PPAR. Glycyrrhizic acid (GA, a triterpenoid saponin, is the primary bioactive constituent of the roots of the shrub Glycyrrhiza glabra. Studies have indicated that triterpenoids could act as PPAR agonists and GA is therefore postulated to restore LPL expression in the insulin resistant state. Results Oral administration of 100 mg/kg of GA to high-fat diet-induced obese rats for 28 days led to significant reduction in blood glucose concentration and improvement in insulin sensitivity as indicated by the homeostasis model assessment of insulin resistance (HOMA-IR (p Conclusion In conclusion, GA may be a potential compound in improving dyslipidaemia by selectively inducing LPL expression in non-hepatic tissues. Such up-regulation was accompanied by a GA-mediated improvement in insulin sensitivity, which may be associated with a decrease in tissue lipid deposition. The HDL-raising effect of GA suggests the antiatherosclerotic properties of GA.

  13. Comparative Study of Tomato and Tomato Paste Supplementation on the Level of Serum Lipids and Lipoproteins Levels in Rats Fed With High Cholesterol

    OpenAIRE

    Khayat Nouri, Mir Hadi; Namvaran Abbas Abad, Ali

    2013-01-01

    Background Hypercholesterolemia is one of the risk factors of cardiovascular diseases. Increased blood cholesterol affects general health and increases mortality due to cardiovascular disease. Poor nutrition increases LDL cholesterol and decreases LDL receptor activities in the liver. Scientists have shown that consumption of antioxidants can reduce hypercholesterolemia and proved benefits of fruit and vegetables. Tomato reduces oxidative stress by increasing serum total antioxidant level. Ob...

  14. Milk minerals modify the effect of fat intake on serum lipid profile: results from an animal and a human short-term study

    DEFF Research Database (Denmark)

    Lorenzen, Janne K; Jensen, Søren Krogh; Astrup, Arne

    2014-01-01

    Despite a high content of saturated fat, evidence from observational studies indicates that the consumption of dairy products may have a neutral effect or may be inversely associated with the risk of CVD. We aimed to examine whether milk minerals modify the effect of saturated fat on serum lipid...... profile. We present data from two studies. Study I had a randomised, blinded, parallel design (n 24 pigs) with a 10 d adaptation period during which a high-fat diet was fed to the pigs and a 14 d intervention period during which the same diet either enriched with milk minerals (MM group) or placebo...... (control group) was fed to the pigs. Study II had a randomised cross-over design (n 9 men) where the subjects were fed either a high-fat diet enriched with milk minerals (MM period) or a regular diet (control period). In both the studies, blood variables were measured before and after the intervention and...

  15. The Role of Breastfeeding in Postpartum Weight Loss; Relationship between Maternal Plasma Ghrelin, Serum Leptin and Lipid Levels with Breast Milk

    Directory of Open Access Journals (Sweden)

    Karataş Z et al.

    2012-10-01

    Full Text Available Objective: The aim of this study is to examine the relationship between blood ghrelin, leptin and lipid levels of breast milk and maternal weight loss in postpartum period. Methods: Sixty-two mothers were subdivided according to babies dietary patterns; as breastfed (BF, BF plus formula-fed (BF+FF and formula-fed (FF. All participating mothers were evaluated twice. Results: On the first visit, in BF group total/active ghrelin ratio and serum leptin levels were lower than FF group (p<0.05. The milk leptin levels of BF group were higher than BF+FF group on both visits (p<0.001, p<0.05 respectively. On the second visit, in BF group, body mass index (BMI were decreased when compared with first visit (p<0.05. On the first visit, there was no correlation between milk leptin levels and BMI in lactating mothers. However a negative correlation between milk leptin and total cholesterol levels in lactating mothers (p<0.01 and positive correlation between milk active ghrelin and triglyceride levels in BF group (p<0.05. There was no correlation between ghrelin, leptin and lipid levels in breast milk and maternal blood. Conclusion: The low serum leptin levels and total/active ghrelin in lactating mothers than non-lactating are thought the role of these hormones, which passes to the baby through breast milk, on losing weight. Additionally, the higher milk leptin levels in only BF group than BF+FF group, shows that the role of protective effect of leptin in only breastfeeding infants. Breastfeeding should be prompted more to prevent obesity.

  16. Rosuvastatin therapy does not affect serum MMP-13 or TIMP-1 levels in hypercholesterolemic patients.

    Science.gov (United States)

    Cevik, Cihan; Nugent, Kenneth; Meyerrose, Gary; Otahbachi, Mohammad; Izgi, Cemil; Lyte, Mark; Fish, R David

    2011-01-01

    Matrix metalloproteinases degrade the collagen content of atherosclerotic plaque and reduce plaque stability. In tissue sections of atherosclerotic plaque, the expression of matrix metalloproteinases is increased. 3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) decrease the tissue expression of matrix metalloproteinases-1, -2, -3, and -9 in atheromatous plaque by attenuating the inflammatory process that leads to increased expression. However, it is not known whether statins decrease levels of matrix metalloproteinase-13--an enzyme crucial to the initiation of collagen degradation-as part of their plaque-stabilizing effect.We prospectively examined the effect of statin therapy on serum levels of matrix metalloproteinase-13, tissue inhibitor of metalloproteinase-1, and low-density-lipoprotein cholesterol in 14 patients with hypercholesterolemia. All were at low risk for adverse cardiovascular events and were given 20 mg/d of rosuvastatin for 4 weeks. Post-therapy levels of matrix metalloproteinase-13 and tissue inhibitor of metalloproteinase-1 were compared with baseline levels. Although low-density-lipoprotein cholesterol levels were significantly decreased in the 14 patients (mean baseline level, 152 ± 21 mg/dL vs mean post-therapy level, 73 ± 45 mg/dL; P < 0.001), matrix metalloproteinase-13 and tissue inhibitor of metalloproteinase-1 levels were unchanged (matrix metalloproteinase-13, 0.295 ± 0.06 ng/mL vs 0.323 ± 0.11 ng/mL, P = 0.12; and tissue inhibitor of metalloproteinase-1, 400.8 ± 43.4 ng/mL vs 395.3 ± 47.5 ng/mL, P = 0.26). We conclude that even though there was a decrease in low-density-lipoprotein cholesterol, short-term, high-dose rosuvastatin therapy has no effect on matrix metalloproteinase-13 and tissue inhibitor of metalloproteinase-1 levels in hypercholesterolemic patients. However, further investigation is warranted. PMID:21720458

  17. Calcium and protons affect the interaction of neurotransmitters and anesthetics with anionic lipid membranes.

    Science.gov (United States)

    Pérez-Isidoro, Rosendo; Ruiz-Suárez, J C

    2016-09-01

    We study how zwitterionic and anionic biomembrane models interact with neurotransmitters (NTs) and anesthetics (ATs) in the presence of Ca(2+) and different pH conditions. As NTs we used acetylcholine (ACh), γ-aminobutyric acid (GABA), and l-glutamic acid (LGlu). As ATs, tetracaine (TC), and pentobarbital (PB) were employed. By using differential scanning calorimetry (DSC), we analyzed the changes such molecules produce in the thermal properties of the membranes. We found that calcium and pH play important roles in the interactions of NTs and ATs with the anionic lipid membranes. Changes in pH promote deprotonation of the phosphate groups in anionic phospholipids inducing electrostatic interactions between them and NTs; but if Ca(2+) ions are in the system, these act as bridges. Such interactions impact the physical properties of the membranes in a similar manner that anesthetics do. Beyond the usual biochemical approach, we claim that these effects should be taken into account to understand the excitatory-inhibitory orchestrated balance in the nervous system. PMID:27362370

  18. Relation of serum cholesterol, lipid, serotonin and tryptophan levels to severity of depression and to suicide attempts.

    OpenAIRE

    Almeida-Montes, L G; Valles-Sanchez, V; Moreno-Aguilar, J; Chavez-Balderas, R A; García-Marín, J A; Cortés Sotres, J F; Hheinze-Martin, G

    2000-01-01

    OBJECTIVE: To determine if there is a relation to low serum cholesterol, lipoprotein, serotonin or tryptophan levels in patients with depression who have recently attempted suicide. DESIGN: Biochemical and behavioural study. SETTING: Inpatient and outpatient treatment at the Instituto Mexicano de Psiquiatría. PARTICIPANTS: Thirty-three patients with a diagnosis of major depressive episode. Eighteen of these patients had attempted suicide in the month before the start of the study; 15 patients...

  19. [Relationship between serum lipids and status of vitamin C and E as antioxidants in Venezuelan elderly people].

    Science.gov (United States)

    Meertens, Lesbia; Ruido, Tathiana; Díaz, Nayka; Naddaf, Gloria; Rodríguez, Adelmo; Solano, Liseti

    2008-12-01

    During aging there is a tendency towards hyperlipidemia and changes in the distribution of lipoproteins. A decline in the functioning of the body's antioxidant defense system is also observed at this time. The objective of this study was to establish the relationship between serum concentrations of total cholesterol and fractions, triglycerides, and Vitamins C and E. 61 adults over 60 years of age were evaluated from January to March, 2006. Nutritional status was diagnosed by BMI (WHO); serum levels of triglycerides (TG), total cholesterol (TC) and fractions (HDL-c and LDL-c) were determined by enzyme method; Vitamin C (colorimetric method) and Vitamin E by HPLC. ATPIII values were used as a reference for risk of TG, TC, HDL, LDL-c, vitamin C: > 0.9 mg/dL (normal), < 0.9 mg/dL (deficit); vitamin E: = 1300 microg/dL (normal), 1300 = microg /dL (deficit). Consumption of vitamins C and E were estimated by the direct weighing method 3 days per week. According to BMI, 19.7% had nutritional deficit, 39.3% overweight, and 11.5% obesity. TG, TC, LDL-c levels were at risk in females, and HDL-c in both genders. Prevalence of risk for heart disease was: TG (45.2%), HDL-c (51.1%), and LDL-c (52.5%). Consumption and serum levels of vitamin E were low in both genders. There was no association between variables. A significant and positive correlation between TG, TC, LDL-C, serum vitamin E, and BMI was observed. The female group showed overweight, hypertriglyceridemia and hypercholesterolemia, HDL-c and LDL-c at risk, and vitamin E deficiency, all of which are important risk factors for cardiovascular disease in this age group. PMID:19368297

  20. Cholesterol strongly affects the organization of lipid monolayers studied as models of the milk fat globule membrane: Condensing effect and change in the lipid domain morphology.

    Science.gov (United States)

    Murthy, Appala Venkata Ramana; Guyomarc'h, Fanny; Paboeuf, Gilles; Vié, Véronique; Lopez, Christelle

    2015-10-01

    The biological membrane that surrounds the milk fat globules exhibits phase separation of polar lipids that is poorly known. The objective of this study was to investigate the role played by cholesterol in the organization of monolayers prepared as models of the milk fat globule membrane (MFGM). Differential scanning calorimetry and X-ray diffraction experiments allowed characterization of the gel to liquid crystalline phase transition temperature of lipids, Tm ~35°C, in vesicles prepared with a MFGM lipid extract. For temperature below Tm, atomic force microscopy revealed phase separation of lipids at 30 mN·m(-1) in Langmuir-Blodgett monolayers of the MFGM lipid extract. The high Tm lipids form liquid condensed (LC) domains that protrude by about 1.5 nm from the continuous liquid expanded (LE) phase. Cholesterol was added to the MFGM extract up to 30% of polar lipids (cholesterol/milk sphingomyelin (MSM) molar ratio of 50/50). Compression isotherms evidenced the condensing effect of the cholesterol onto the MFGM lipid monolayers. Topography of the monolayers showed a decrease in the area of the LC domains and in the height difference H between the LC domains and the continuous LE phase, as the cholesterol content increased in the MFGM lipid monolayers. These results were interpreted in terms of nucleation effects of cholesterol and decrease of the line tension between LC domains and LE phase in the MFGM lipid monolayers. This study revealed the major structural role of cholesterol in the MFGM that could be involved in biological functions of this interface (e.g. mechanisms of milk fat globule digestion). PMID:26087463

  1. Effects of supplementation with a calcium-rich marine-derived multi-mineral supplement and short-chain fructo-oligosaccharides on serum lipids in postmenopausal women.

    Science.gov (United States)

    Cronin, Barbara E; Allsopp, Philip J; Slevin, Mary M; Magee, Pamela J; Livingstone, M Barbara E; Strain, J J; McSorley, Emeir M

    2016-02-28

    Recent literature suggests that Ca supplements have adverse effects on cardiovascular health. The effects of a Ca-rich supplement administered alone or in combination with short-chain fructo-oligosaccharides (scFOS) on serum lipids in postmenopausal women were examined using secondary data from a 24-month double-blind randomised controlled study. A total of 300 postmenopausal women were randomly assigned to daily supplements of 800 mg of Ca (2·4 g Aquamin) (Ca), 800 mg of Ca with 3 g of scFOS (CaFOS) or control (maltodextrin) (MD). A full lipid profile, body composition, blood pressure and a range of cytokines were measured at baseline and after 24 months. Intention-to-treat ANCOVA assessed treatment effects between the groups. A significant time-by-treatment effect was observed for LDL and total cholesterol for the Ca and CaFOS groups, with both groups having lower LDL and total cholesterol concentrations compared with MD after 24 months. The control group had mean (5·2 mmol/l) total cholesterol concentrations above the normal range (≤ 5 mmol/l) at 24 months, whereas values remained within the normal range in the treatment groups. There was no significant treatment effect on HDL-cholesterol, TAG, body composition, blood pressure or cytokine concentrations at 24 months, with the exception of IL-4, where there was a significant increase in the CaFOS group compared with the placebo. This study demonstrates a lipid-lowering effect of both the Ca-rich supplement alone and the supplement with scFOS. At the 4-year follow-up, there was no significant difference between the groups for reported diagnosed cardiovascular conditions. PMID:26669430

  2. Immunofluorescence in multiple tissues utilizing serum from a patient affected by systemic lupus erythematosus

    Directory of Open Access Journals (Sweden)

    Piotr Brzezinski

    2012-01-01

    Full Text Available Introduction: Lupus erythematosus is a chronic, inflammatory autoimmune disease that can affect multiple organs. Lupus can affect many parts of the body, especially in systemic lupus erythematosus (SLE; affected tissues may include the joints, skin, kidneys, heart, lungs, blood vessels, and brain. Case report: A 46-year-old female presented with pruritus, photosensitivity and edema of the cheeks of about 2 years duration, and was evaluated by a dermatologist. On examination, multiple telangiectasias were present on the cheeks, with erythema, edema and a malar rash observed. A review of systems documented breathing difficulty and pleuitic pain, joint pain and joint edema, photosensitivity, cardiac dysrhythmia, and periodic pain in the back close to the kidneys. Methods: Skin biopsies for hematoxylin and eosin testing, as well for direct and indirect immunofluorescence were performed, in addition to multiple diagnostic blood tests, chest radiography and directed immunologic testing. Results: The blood testing showed elevated C-reactive protein. Direct and indirect immunofluorescence testing utilizing monkey esophagus, mouse and pig heart and kidney, normal human eyelid skin and veal brain demonstrated strong reactivity to several components of smooth muscle, nerves, blood vessels, skin basement membrane zone and sweat gland ducts and skin meibomian glands. Anti-endomysium antibodies were detected as well as others, especially using FITC conjugated Complement/C1q, FITC conjugated anti-human immunoglobulin IgG and FITC conjugated anti-human fibrinogen. Conclusions: We conclude that both direct and indirect immunofluorescence using several substrates can unveil previously undocumented autoantibodies in multiple organs in lupus erythematosus, and that these findings could be utilized to complement existing diagnostic testing for this disorder.

  3. The effects of prebiotic, probiotic and synbiotic diets containing Bacillus coagulans and inulin on serum lipid profile in the rat

    OpenAIRE

    Khadijeh Abhari; Seyed Shahram Shekarforoush; Saeed Hosseinzadeh; Saeed Nazifi; Javad Sajedianfard

    2015-01-01

    An in vivo trial was conducted to evaluate the effects of Bacillus coagulans, and inulin, either separately or in combination, on lipid profile using a rat model. Thirty-two male Wistar rats were randomly divided into four groups (n=8) and fed as follows: standard diet (control), standard diet with 5% w/w long chain inulin (prebiotic), standard diet with 109 spores/day spores of B. coagulans by orogastric gavage (probiotic), and standard diet with 5% w/w long chain inulin and 109 spores/day o...

  4. Effects of cerium oxide supplementation to laying hen diets on performance, egg quality, some antioxidant enzymes in serum and lipid oxidation in egg yolk.

    Science.gov (United States)

    Bölükbaşı, S C; Al-Sagan, A A; Ürüşan, H; Erhan, M K; Durmuş, O; Kurt, N

    2016-08-01

    This study was conducted to determine the effects of dietary cerium oxide levels (0, 100, 200, 300 or 400 mg/kg) on the laying performance, egg quality, some blood serum parameters and egg lipid peroxidation of laying hen. In total, one hundred and twenty 22-week-old brown Lohman LSL laying hens were randomly assigned to five groups equally (n = 24). Each treatment was replicated six times. Dietary supplementation of cerium oxide had no significant effect on feed intake and egg weight. The addition of cerium oxide to the laying hens' feed improved feed conversion ratio and increased (p laying hens feed led to a significant (p hen diets. It was also observed that serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentration decreased significantly with supplementation of cerium oxide in diets. Inclusion of cerium oxide resulted in a significant reduction in thiobarbituric acid reactive substance (TBARS) values in egg yolk in this study. It can be concluded that the addition of cerium oxide had positive effects on egg production, feed conversion ratio and egg shelf life. Based on the results of this study, it could be advised to supplement laying hens feed with cerium oxide as feed additives. PMID:26847677

  5. The Effect of Hydro-Alcoholic Extract of Grape Seed (Vitis vinifera on Sugar and Lipids in Serum of Diabetic Rats

    Directory of Open Access Journals (Sweden)

    Zohre Ganjali

    2012-06-01

    Full Text Available Nowadays, no-drug treatments (medicinal plants are novel therapeutic approaches in the treatment of diabetes. This study aimed at assessing the effect of Grape Seed extract on the blood glucose and lipid profile in diabetic rats. Thirty male adult rats were randomly selected and divided into three groups as nondiabetic control; diabetic control; diabetic rats treated with hydroalcoholic extract of grape seed. In diabetic groups, alloxan monohydrate (120 mg/kg was injected intraperitoneally to develop diabetes. Then the Test group received intraperitoneal injection of hydro-alcoholic extract of grape seed (100 mg/kg. At last, glucose, cholesterol, triglyceride, HDL, VLDL, LDL and insulin contents of the rats' serum sample were determined. Diabetic rats treated with extract showed a significant decrease in blood glucose level (p<0.05. Furthermore, compared diabetic group, in the extract-treated rats, there was a significant decrease in serum contents of total cholesterol (TC ,LDL,VLDL and TG, but a significant increase in insulin level and HDL (p<0.05. These results show that the hydroalcoholic extract of Grape Seed may be effective in the treatment of diabetes. This effect can be due to the presence of flavonoides and their antioxidant features.

  6. Methylation on the Circadian Gene BMAL1 Is Associated with the Effects of a Weight Loss Intervention on Serum Lipid Levels.

    Science.gov (United States)

    Samblas, Mirian; Milagro, Fermin I; Gómez-Abellán, Purificación; Martínez, J Alfredo; Garaulet, Marta

    2016-06-01

    The circadian clock system has been linked to the onset and development of obesity and some accompanying comorbidities. Epigenetic mechanisms, such as DNA methylation, are putatively involved in the regulation of the circadian clock system. The aim of this study was to investigate the influence of a weight loss intervention based on an energy-controlled Mediterranean dietary pattern in the methylation levels of 3 clock genes, BMAL1, CLOCK, and NR1D1, and the association between the methylation levels and changes induced in the serum lipid profile with the weight loss treatment. The study sample enrolled 61 women (body mass index = 28.6 ± 3.4 kg/m(2); age: 42.2 ± 11.4 years), who followed a nutritional program based on a Mediterranean dietary pattern. DNA was isolated from whole blood obtained at the beginning and end point. Methylation levels at different CpG sites of BMAL1, CLOCK, and NR1D1 were analyzed by Sequenom's MassArray. The energy-restricted intervention modified the methylation levels of different CpG sites in BMAL1 (CpGs 5, 6, 7, 9, 11, and 18) and NR1D1 (CpGs 1, 10, 17, 18, 19, and 22). Changes in cytosine methylation in the CpG 5 to 9 region of BMAL1 with the intervention positively correlated with the eveningness profile (p = 0.019). The baseline methylation of the CpG 5 to 9 region in BMAL1 positively correlated with energy (p = 0.047) and carbohydrate (p = 0.017) intake and negatively correlated with the effect of the weight loss intervention on total cholesterol (p = 0.032) and low-density lipoprotein cholesterol (p = 0.005). Similar significant and positive correlations were found between changes in methylation levels in the CpG 5 to 9 region of BMAL1 due to the intervention and changes in serum lipids (p lipids levels. PMID:26873744

  7. Rutin ameliorates glycemic index, lipid profile and enzymatic activities in serum, heart and liver tissues of rats fed with a combination of hypercaloric diet and chronic ethanol consumption.

    Science.gov (United States)

    Chuffa, Luiz Gustavo A; Fioruci-Fontanelli, Beatriz A; Bordon, Juliana G; Pires, Rafaelle B; Braga, Camila P; Seiva, Fábio R F; Fernandes, Ana Angélica H

    2014-06-01

    Alcoholism and obesity are strongly associated with several disorders including heart and liver diseases. This study evaluated the effects of rutin treatment in serum, heart and liver tissues of rats subjected to a combination of hypercaloric diet (HD) and chronic ethanol consumption. Rats were divided into three groups: Control: rats fed a standard diet and drinking water ad libitum; G1: rats fed the HD and receiving a solution of 10% (v/v) ethanol; and G2: rats fed the HD and ethanol solution, followed by injections of 50 mg/kg(-1) rutin as treatment. After 53 days of HD and ethanol exposure, the rutin was administered every three days for nine days. At the end of the experimental period (95 days), biochemical analyses were carried out on sera, cardiac and hepatic tissues. Body weight gain and food consumption were reduced in both the G1 and G2 groups compared to control animals. Rutin effectively reduced the total lipids (TL), triglycerides (TG), total cholesterol (TC), VLDL, LDL-cholesterol and glucose levels, while it increased the HDL-cholesterol in the serum of G2 rats, compared to G1. Although rutin had no effect on total protein, albumin, uric acid and cretinine levels, it was able to restore serum activities of alkaline phosphatase (ALP), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatine kinase (CK) in animals fed HD and receiving ethanol. Glycogen stores were replenished in both hepatic and cardiac tissues after rutin treatment. Moreover, rutin consistently reduced hepatic levels of TG and TC and cardiac AST, ALT and CK activities. Thus, rutin treatment was effective in reducing the risk factors for cardiac and hepatic disease caused by both HD and chronic ethanol consumption. PMID:25204084

  8. Apolipoprotein M affecting lipid metabolism or just catching a ride with lipoproteins in the circulation?

    DEFF Research Database (Denmark)

    Dahlbäck, B; Nielsen, Lars Bo

    2009-01-01

    link to cholesterol metabolism. However, in casecontrol studies, apoM levels in patients with coronary heart disease (CHD) and controls were similar, suggesting apoM levels not to affect the risk for CHD in humans. Experiments in transgenic mice suggested apoM to have antiatherogenic properties......; possible mechanisms include increased formation of pre-ss HDL, enhanced cholesterol mobilization from foam cells, and increased antioxidant properties....

  9. Relationship between serum low-density lipoprotein cholesterol and in-hospital mortality following acute myocardial infarction (the lipid paradox).

    Science.gov (United States)

    Reddy, Vanessa S; Bui, Quang T; Jacobs, Joan R; Begelman, Susan M; Miller, Dave P; French, William J

    2015-03-01

    Lipoprotein levels are currently recognized as independent risk factors for long-term cardiovascular events after acute myocardial infarction (AMI). During the acute-phase reaction after AMI, previous studies have reported trends of decreased low-density lipoprotein cholesterol (LDL-C), increased triglycerides, and variable high-density lipoprotein cholesterol (HDL-C) levels. However, the association between LDL-C and HDL-C levels and in-hospital mortality has not been well established following AMI. The relationship between lipid levels and in-hospital all-cause mortality in 115,492 patients hospitalized for AMI (July 2002 to December 2006), registered in the National Registry of Myocardial Infarction (NRMI) 4b-5, was evaluated using multivariable-adjusted logistic regression models. Mean LDL-C was 104 ± 38, HDL-C was 41 ± 14, and triglycerides 143 ± 83 mg/dl. Compared with the lowest quartile of LDL-C (NRMI 4b-5 suggest a lipid paradox, with lower LDL-C levels associated with increased risk of in-hospital mortality, contrary to findings outside the acute setting. Consistent with previous analyses, lowest HDL-C levels were associated with increased in-hospital mortality. In conclusion, further explorations of the relationship between very low levels of LDL-C, myocardial necrosis, and subsequent adverse cardiovascular events are warranted. PMID:25727079

  10. Peripheral vagus nerve stimulation significantly affects lipid composition and protein secondary structure within dopamine-related brain regions in rats.

    Science.gov (United States)

    Surowka, Artur Dawid; Krygowska-Wajs, Anna; Ziomber, Agata; Thor, Piotr; Chrobak, Adrian Andrzej; Szczerbowska-Boruchowska, Magdalena

    2015-06-01

    Recent immunohistochemical studies point to the dorsal motor nucleus of the vagus nerve as the point of departure of initial changes which are related to the gradual pathological developments in the dopaminergic system. In the light of current investigations, it is likely that biochemical changes within the peripheral nervous system may influence the physiology of the dopaminergic system, suggesting a putative role for it in the development of neurodegenerative disorders. By using Fourier transform infrared microspectroscopy, coupled with statistical analysis, we examined the effect of chronic, unilateral electrical vagus nerve stimulation on changes in lipid composition and in protein secondary structure within dopamine-related brain structures in rats. It was found that the chronic vagal nerve stimulation strongly affects the chain length of fatty acids within the ventral tegmental area, nucleus accumbens, substantia nigra, striatum, dorsal motor nucleus of vagus and the motor cortex. In particular, the level of lipid unsaturation was found significantly increasing in the ventral tegmental area, substantia nigra and motor cortex as a result of vagal nerve stimulation. When it comes to changes in protein secondary structure, we could see that the mesolimbic, mesocortical and nigrostriatal dopaminergic pathways are particularly affected by vagus nerve stimulation. This is due to the co-occurrence of statistically significant changes in the content of non-ordered structure components, alpha helices, beta sheets, and the total area of Amide I. Macromolecular changes caused by peripheral vagus nerve stimulation may highlight a potential connection between the gastrointestinal system and the central nervous system in rat during the development of neurodegenerative disorders. PMID:25893743

  11. A High Phosphorus Diet Affects Lipid Metabolism in Rat Liver: A DNA Microarray Analysis.

    Directory of Open Access Journals (Sweden)

    Sunwoo Chun

    Full Text Available A high phosphorus (HP diet causes disorders of renal function, bone metabolism, and vascular function. We previously demonstrated that DNA microarray analysis is an appropriate method to comprehensively evaluate the effects of a HP diet on kidney dysfunction such as calcification, fibrillization, and inflammation. We reported that type IIb sodium-dependent phosphate transporter is significantly up-regulated in this context. In the present study, we performed DNA microarray analysis to investigate the effects of a HP diet on the liver, which plays a pivotal role in energy metabolism. DNA microarray analysis was performed with total RNA isolated from the livers of rats fed a control diet (containing 0.3% phosphorus or a HP diet (containing 1.2% phosphorus. Gene Ontology analysis of differentially expressed genes (DEGs revealed that the HP diet induced down-regulation of genes involved in hepatic amino acid catabolism and lipogenesis, while genes related to fatty acid β-oxidation process were up-regulated. Although genes related to fatty acid biosynthesis were down-regulated in HP diet-fed rats, genes important for the elongation and desaturation reactions of omega-3 and -6 fatty acids were up-regulated. Concentrations of hepatic arachidonic acid and eicosapentaenoic acid were increased in HP diet-fed rats. These essential fatty acids activate peroxisome proliferator-activated receptor alpha (PPARα, a transcription factor for fatty acid β-oxidation. Evaluation of the upstream regulators of DEGs using Ingenuity Pathway Analysis indicated that PPARα was activated in the livers of HP diet-fed rats. Furthermore, the serum concentration of fibroblast growth factor 21, a hormone secreted from the liver that promotes fatty acid utilization in adipose tissue as a PPARα target gene, was higher (p = 0.054 in HP diet-fed rats than in control diet-fed rats. These data suggest that a HP diet enhances energy expenditure through the utilization of free fatty

  12. Sitamaquine-resistance in Leishmania donovani affects drug accumulation and lipid metabolism.

    Science.gov (United States)

    Imbert, L; Cojean, S; Libong, D; Chaminade, P; Loiseau, P M

    2014-09-01

    This study focuses on the mechanism of sitamaquine-resistance in Leishmania donovani. Sitamaquine accumulated 10 and 1.4 fold more in cytosol than in membranes of wild-type (WT) and of sitamaquine-resistant (Sita-R160) L. donovani promastigotes, respectively. The sitamaquine accumulation was a concentration-dependent process in WT whereas a saturation occurred in Sita-R160 suggesting a reduced uptake or an increase of the sitamaquine efflux. Membrane negative phospholipids being the main target for sitamaquine uptake, a lipidomic analysis showed that sitamaquine-resistance did not rely on a decrease of membrane negative phospholipid rate in Sita-R160, discarding the hypothesis of reduced uptake. However, sterol and phospholipid metabolisms were strongly affected in Sita-R160 suggesting that sitamaquine-resistance could be related to an alteration of phosphatidylethanolamine-N-methyl-transferase and choline kinase activities and to a decrease in cholesterol uptake and of ergosterol biosynthesis. Preliminary data of proteomics analysis exhibited different protein profiles between WT and Sita-160R remaining to be characterized. PMID:25201056

  13. Clinical trial of Cecropia obtusifolia and Marrubium vulgare leaf extracts on blood glucose and serum lipids in type 2 diabetics.

    Science.gov (United States)

    Herrera-Arellano, A; Aguilar-Santamaría, L; García-Hernández, B; Nicasio-Torres, P; Tortoriello, J

    2004-11-01

    Cecropia obtusifolia and Marrubium vulgare have been widely used in Mexican traditional medicine for the control of type 2 diabetes. In order to evaluate the clinical effect produced by the aqueous extract from these species on type 2 non-controlled diabetes mellitus, a total of 43 outpatients were included. Based on the European NIDDM (policy group) criteria, only patients with poor response to the conventional treatment were selected. All patients maintained their medical treatment and also received a prepared infusion of the dry leaves of the plant treatment for 21 days. In a double-blind manner, the patients were randomly grouped as follows: 22 patients were treated with C. obtusifolia and 21 with M. vulgare. The fasting blood glucose values were reduced by 15.25% on patients treated with C. obtusifolia, while cholesterol and triglycerides were decreased by 14.62% and 42.0%, respectively (ANOVA pvulgare, the plasma glucose level was reduced by 0.64% and cholesterol and triglycerides by 4.16% and 5.78%, respectively. When the results were compared between groups, significant differences in glucose and cholesterol diminution were found. The obtained results showed that the infusion prepared with the leaves of C. obtusifolia (containing 2.99+/-0.14mg of chlorogenic acid/g of dried plant) produced beneficial effects on carbohydrate and lipid metabolisms when it was administered as an adjunct on patients with type 2 diabetes with poor response to conventional medical treatment. PMID:15636168

  14. Study of changes in serum lipid profile and blood sugar level by perindopril and telmisartan during treatment of systemic hypertension

    Directory of Open Access Journals (Sweden)

    Manish Kumar

    2014-06-01

    Results: With perindopril initial means of TC, HDL, LDL, TGs, FBS, and PPBS in Groups A and B were 190.32, 49.76, 117.96, 165.04, 84.56, 122.60, and 188.80, 51.64, 118.52, 159.12, 93.92, 133.60, respectively. After 24 weeks, these values were 190.84, 50.68, 118.60, 163.84, 83.48, 120.20, and 190.96, 52.04, 118.28, 157.56, 93.96, 133.68, respectively (p > 0.05. With telmisartan, initial means of TC, HDL, LDL, TG, FBS, and PPBS in both groups were 188.08, 49.76, 118.84, 167.20, 83.72, 120.68, and 188.08, 46.88, 121.96, 167.84, 91.44, 131.72, respectively. After 24 weeks, these values in both groups were 189.36, 49.80, 120.04, 165.96, 82.60, 118.36 and 186.12, 45.28, 121.08, 167.72, 92.76, 129.56 respectively (p > 0.05. Conclusions: It concluded that both perindopril and telmisartan had not any significant adverse effects on plasma lipid profile and blood sugar level in both groups. [Int J Basic Clin Pharmacol 2014; 3(3.000: 454-459

  15. Effects of Low-Dose Pioglitazone on Serum Levels of Adiponectin, Dehydroepiandrosterone, Amyloid Beta Peptide, and Lipid Profile in Elderly Japanese People with Type 2 Diabetes

    Directory of Open Access Journals (Sweden)

    Yuji Aoki

    2015-01-01

    Full Text Available This study was performed to see how pioglitazone at low doses could affect blood biomarkers related to atherosclerosis and aging. The effects of an add-on treatment with pioglitazone (15 mg for males and 7.5 mg for females for 6 months were assessed in 24 outpatients (12 males, 12 females with type 2 diabetes aged ≥ 70 years. As doses of sulfonylurea were reduced in 10 patients, no significant differences in HbA1c and glucose levels were seen. After the treatment, serum levels of HDL cholesterol, arachidonic acid (predominant in males, and high-molecular-weight adiponectin significantly increased. The level of dehydroepiandrosterone sulfate significantly decreased. No significant changes were seen in those of small dense LDL cholesterol, high-sensitivity C-reactive protein, and amyloid beta peptides 1–40 and 1–42. There was a slight but significant increase in body weight, but apparent adverse effects were not observed. In conclusion, pioglitazone at low doses increased serum adiponectin, HDL cholesterol, and arachidonic acid levels but decreased serum dehydroepiandrosterone level, not associated with glycemia, in elderly Japanese people with type 2 diabetes. An optimal dose of pioglitazone should be sought for to minimize its adverse effects and to fully exert its pleiotropic effects such as antiatherosclerotic and antiaging effects.

  16. Relation of electrocardiographic left ventricular hypertrophy to blood pressure, body mass index, serum lipids and blood sugar levels in adult Nigerians.

    Science.gov (United States)

    Opadijo, O G; Omotoso, A B O; Akande, A A

    2003-12-01

    Left ventricular hypertrophy (LVH) is considered an independent risk factor even in the absence of systemic hypertension. Electrocardiographic (ECG) LVH with repolarisation changes has been found in some countries to carry more coronary risk than LVH alone. How far this observation is true among adult Nigerians is not known. We therefore decided to study adult Nigerians with ECG-LVH with or without ST-T waves changes and compare them with normal age matched controls (without ECG-LVH) in relation with established modifiable risk factors such as systemic hypertension (BP), body mass index (BMI), fasting blood sugar (FBS) and serum lipids such as total cholesterol (Tc), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG). Adult Nigerians who were consecutively referred to the ECG laboratory were randomly recruited. Three hundred patients were studied. Their blood pressures (BP) as well as body mass indices were recorded after recording their resting 12 read ECG using portable Seward 9953 ECG machine. Their waist-hip ratio (WHR) was also recorded. Blood samples were taken to determine their fasting blood sugar and serum lipids. Their ECG tracings were read by the cardiologists involved in the study while the blood samples were analysed by the chemical pathologist also involved in the study. At the end of the ECG reading, the patients were divided into 3 groups according to whether there was no ECG-LVH (control group A), ECG-LVH alone (group B), and ECG-LVH with ST-T waves changes (group C). One hundred and fifty (50%) patients belonged to group A, 100 (33.3%) patients to group B and 50 (16.7%) group C. Group B patients were found to have higher modifiable risk factors in form of systemic BP. Tc, LDL-C, and WHR compared to group A. However, the group C patients had increased load of these coronary risk factors in terms of BP elevation, higher BMI, FBS, and scrum cholesterol compared to group B. In addition

  17. Relationships between abdominal fat distribution assessed by computer tomography, body composition, serum lipids, plasma glucose and cardiorespiratory functions in obese children

    International Nuclear Information System (INIS)

    Visceral abdominal fat to subcutaneous abdominal fat ratio (V/S ratio) is a predictor for cardiac disease, metabolic disease, and hypertension in obese adults. This study determined the histopathological value of V/S ratio in obese children using computed tomography (CT). The subjects were 37 boys and 26 girls with overweight by more than 20%, whose ages ranged from 3 to 16 years. Although the percentage of standard body weight (SBW), percentage of body fat (BF), and body mass index (BMI) were correlated with each other, there was no correlation between the V/S ratio and the three predictors. Thus, the V/S ratio is completely different from the other obesity predictors in children. The V/S ratio in children of 20% or more overweight of SBW was 0.28±0.11. The V/S ratio of 0.4, used as an obesity predictor in adults, was not considered suitable in the case of children. Liver function, serum lipid levels, and serum glucose correlated with the percentage of SBW, BMI, and the percentage of BF, but not correlated with the V/S ratio. According to the V/S ratio, the patients were divided into the group of V/S ratio of less than 0.28 (group I, n=34) and the group of V/S ratio of 0.28 or more (group II, n=27). There was no significant difference in age, percentage of SBW, BMI, and percentage of BF. Triglyceride was significantly higher in group II than group I, but there was no significant difference in plasma glucose and other lipids, body composition, blood pressure or respiratory function. Of note, the V/S ratio of 0.4 or more was seen in only 9 of the 61 children (14.7%). These findings suggest that the V/S ratio for children is a predictor different from that in the case of adults. (N.K.)

  18. Effect of the hydroalcoholic extract and juice of Prunus divaricata fruit on blood glucose and serum lipids of normal and streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Minaiyan, M; Ghannadi, A; Movahedian, A; Ramezanlou, P; Osooli, F S

    2014-01-01

    Prunus divaricata (Alloocheh) is a small tree cultivating in Iran, Middle East and central Asia. Prunus genus has many species with anti-oxidant, anti-hyperlipidemia and anti-hyperglycemia effects. In the present study the anti-diabetic and anti-hyperlipidemic effects of P. divaricata fruits were examined in normal and streptozotocin (STZ)-induced diabetic rats. Both groups, control and reference rats received normal saline and glibenclamide respectively. Test groups were treated with Prunus freeze dried juice (PFDJ, 200, 400, 800 mg/kg) and Prunus freeze dried extract (PFDE, 100, 200, 400 mg/kg) started at the 3(rd) day of the experiment and continued for 27 days thereafter. Weight changes of animals were checked periodically. Fasting blood glucose (FBG) level as well as serum triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL) cholesterol were determined. Different treatments had no significant effect on body weight increments of normal rats, while in diabetic rats, PFDJ (800 mg/kg) and PFDE (400 mg/kg) opposed with weight loss. In acute phase of experiment (0-8 h of 3(rd) day), none of tested fractions were effective in reducing FBG and serum lipids of normal rats. During the sub-acute phase (13(th) and 30(th) days) however, the greatest test doses of PFDJ (800 mg/kg) and PFDE (400 mg/kg) induced hypoglycema. In diabetic groups, PFDJ and PFDE, at all test doses, could diminish FBG during sub-acute phase of the experiment. In addition, PFDJ and PFDE at most examined doses could diminish TG significantly and they were also effective on cholesterol derivatives in different magnitude. PMID:26339257

  19. Embryonic exposures of lithium and homocysteine and folate protection affect lipid metabolism during mouse cardiogenesis and placentation.

    Science.gov (United States)

    Han, Mingda; Evsikov, Alexei V; Zhang, Lifeng; Lastra-Vicente, Rosana; Linask, Kersti K

    2016-06-01

    Embryonic exposures can increase the risk of congenital cardiac birth defects and adult disease. The present study identifies the predominant pathways modulated by an acute embryonic mouse exposure during gastrulation to lithium or homocysteine that induces cardiac defects. High dose periconceptional folate supplementation normalized development. Microarray bioinformatic analysis of gene expression demonstrated that primarily lipid metabolism is altered after the acute exposures. The lipid-related modulation demonstrated a gender bias with male embryos showing greater number of lipid-related Gene Ontology biological processes altered than in female embryos. RT-PCR analysis demonstrated significant change of the fatty acid oxidation gene Acadm with homocysteine exposure primarily in male embryos than in female. The perturbations resulting from the exposures resulted in growth-restricted placentas with disorganized cellular lipid droplet distribution indicating lipids have a critical role in cardiac-placental abnormal development. High folate supplementation protected normal heart-placental function, gene expression and lipid localization. PMID:26993217

  20. Parenteral lipids and partial enteral nutrition affect hepatic lipid composition but have limited short term effects on formula-induced necrotizing enterocolitis in preterm piglets

    DEFF Research Database (Denmark)

    Vegge, Andreas; Thymann, Thomas; Lauritzen, Lotte;

    2015-01-01

    Rapid transition from total parenteral nutrition (TPN) to enteral feeding is a risk factor for necrotizing enterocolitis (NEC) in preterm infants. We hypothesized that partial enteral nutrition with colostrum, increased proportion of n-3 polyunsaturated fatty acids (PUFA), or exclusion of lipid i...

  1. SCREENING OF SELECTED OLEAGINOUS YEASTS FOR LIPID PRODUCTION FROM GLYCEROL AND SOME FACTORS WHICH AFFECT LIPID PRODUCTION BY YARROWIA LIPOLYTICA STRAINS

    Directory of Open Access Journals (Sweden)

    Salinee Sriwongchai

    2013-04-01

    Full Text Available The ability of eight yeast strains to utilize glycerol as a sole carbon source and accumulate lipids in a chemically defined medium was screened. Among the yeasts, Yarrowia lipolytica strains DSM 70561 and JDC 335 grew to high cell densities on glycerol. These strains were further tested for lipid accumulation under varying nutritional conditions in Erlenmeyer flasks. The results showed that strains DSM 70561 and JDC 335 accumulated lipids up to 37.1 % and 54.4 % of total cell dry weight, respectively, when the defined medium was supplemented with 1 g/L urea and 2 g/L yeast extract. The lipids accumulated by the two yeasts contained a high proportion of C16:0, C18:1, C18:2 and C18:0 fatty acids. The results suggest that Y. lipolytica strains DSM 70561 and JDC 335 have the potential for converting crude glycerol into fatty acids which can in turn be utilized as substrate for biodiesel production.

  2. Does high offshore petroleum activity affect the lipid composition in wild Atlantic Cod (Gadus morhua) from the North Sea?

    OpenAIRE

    Liu, Li

    2012-01-01

    A combined two step extraction, using hexane/methanol to extract neutral lipids and chloroform/methanol to extract polar lipids, has been developed and validation. Four times methanol wash were done to hexane/methanol extract and merged with chloroform/methanol extract to ensure optimized lipid classes distribution between two solvent systems. Quick freeze of hexane/methanol solvent system by dry ice was adopted to avoid adverse effect of generation of micelle. The glass aminopropyl bonded co...

  3. Versatile lipid profiling by liquid chromatography–high resolution mass spectrometry using all ion fragmentation and polarity switching. Preliminary application for serum samples phenotyping related to canine mammary cancer

    International Nuclear Information System (INIS)

    Graphical abstract: -- Highlights: •Lipidomics, high resolution mass spectrometry, polarity switching, serum, canine mammary cancer. -- Abstract: Lipids represent an extended class of substances characterized by such high variety and complexity that makes their unified analyses by liquid chromatography coupled to either high resolution or tandem mass spectrometry (LC–HRMS or LC–MS/MS) a real challenge. In the present study, a new versatile methodology associating ultra high performance liquid chromatography coupled to high resolution tandem mass spectrometry (UHPLC–HRMS/MS) have been developed for a comprehensive analysis of lipids. The use of polarity switching and “all ion fragmentation” (AIF) have been two action levels particularly exploited to finally permit the detection and identification of a multi-class and multi-analyte extended range of lipids in a single run. For identification purposes, both higher energy collision dissociation (HCD) and in-source CID (collision induced dissociation) fragmentation were evaluated in order to obtain information about the precursor and product ions in the same spectra. This approach provides both class-specific and lipid-specific fragments, enhancing lipid identification. Finally, the developed method was applied for differential phenotyping of serum samples collected from pet dogs developing spontaneous malignant mammary tumors and health controls. A biological signature associated with the presence of cancer was then successfully revealed from this lipidome analysis, which required to be further investigated and confirmed at larger scale

  4. Versatile lipid profiling by liquid chromatography–high resolution mass spectrometry using all ion fragmentation and polarity switching. Preliminary application for serum samples phenotyping related to canine mammary cancer

    Energy Technology Data Exchange (ETDEWEB)

    Gallart-Ayala, H., E-mail: laberca@oniris-nantes.fr [LUNAM, Ecole Nationale Vétérinaire, Agroalimentaire et de l’Alimentation Nantes Atlantique (Oniris), USC 1329 INRA Laboratoire d’Etude des résidus et Contaminants dans les Aliments (LABERCA), Site de la Chantrerie – CS50707, 44307 Nantes cedex 3 (France); Courant, F.; Severe, S.; Antignac, J.-P. [LUNAM, Ecole Nationale Vétérinaire, Agroalimentaire et de l’Alimentation Nantes Atlantique (Oniris), USC 1329 INRA Laboratoire d’Etude des résidus et Contaminants dans les Aliments (LABERCA), Site de la Chantrerie – CS50707, 44307 Nantes cedex 3 (France); Morio, F.; Abadie, J. [LUNAM, Ecole Nationale Vétérinaire, Agroalimentaire et de l’Alimentation Nantes Atlantique (Oniris), Cancers Animaux, Modèles pour la Recherche en Oncologie Comparée (AMaROC), Site de la Chantrerie–CS50707, 44307 Nantes cedex 3 (France); Le Bizec, B. [LUNAM, Ecole Nationale Vétérinaire, Agroalimentaire et de l’Alimentation Nantes Atlantique (Oniris), USC 1329 INRA Laboratoire d’Etude des résidus et Contaminants dans les Aliments (LABERCA), Site de la Chantrerie – CS50707, 44307 Nantes cedex 3 (France)

    2013-09-24

    Graphical abstract: -- Highlights: •Lipidomics, high resolution mass spectrometry, polarity switching, serum, canine mammary cancer. -- Abstract: Lipids represent an extended class of substances characterized by such high variety and complexity that makes their unified analyses by liquid chromatography coupled to either high resolution or tandem mass spectrometry (LC–HRMS or LC–MS/MS) a real challenge. In the present study, a new versatile methodology associating ultra high performance liquid chromatography coupled to high resolution tandem mass spectrometry (UHPLC–HRMS/MS) have been developed for a comprehensive analysis of lipids. The use of polarity switching and “all ion fragmentation” (AIF) have been two action levels particularly exploited to finally permit the detection and identification of a multi-class and multi-analyte extended range of lipids in a single run. For identification purposes, both higher energy collision dissociation (HCD) and in-source CID (collision induced dissociation) fragmentation were evaluated in order to obtain information about the precursor and product ions in the same spectra. This approach provides both class-specific and lipid-specific fragments, enhancing lipid identification. Finally, the developed method was applied for differential phenotyping of serum samples collected from pet dogs developing spontaneous malignant mammary tumors and health controls. A biological signature associated with the presence of cancer was then successfully revealed from this lipidome analysis, which required to be further investigated and confirmed at larger scale.

  5. The Effects of Capparis Spinosa Hydroalcoholic Extract on Blood Glucose and Lipids Serum in Diabetic and Normal Male Rats

    OpenAIRE

    M Negahdarizadeh; Mokhtari, M; JM Malekzadeh; J.Mohammadi

    2011-01-01

    Introduction & Objective: Diabetes mellitus is one of the most common endocrine disorders in the world which affects glucose metabolism in the body. Diabetes mellitus is due to lack of insulin secretion and/or failure in insulin action. Researches conducted in the last few decades on plants have reported anti-diabetic properties for some herbs and their traditional use for diabetes treatment. Capparis spinosa is one of these herbs which are used as an anti-diabetic treatment in tribal medici...

  6. Pulmonary surfactant proteins and polymer combinations reduce surfactant inhibition by serum

    OpenAIRE

    Lu, Karen W.; Pérez-Gil, Jesús; Echaide, Mercedes; Taeusch, H. William

    2011-01-01

    Acute respiratory distress syndrome (ARDS) is an inflammatory condition that can be associated with capillary leak of serum into alveoli causing inactivation of surfactant. Resistance to inactivation is affected by types and concentrations of surfactant proteins, lipids, and polymers. Our aim was to investigate the effects of different combinations of these three components. A simple lipid mixture (DPPC/POPG) or a more complex lipid mixture (DPPC/POPC/POPG/cholesterol) was used. Native surfac...

  7. Background Intestinal 18F-FDG Uptake Is Related to Serum Lipid Profile and Obesity in Breast Cancer Patients.

    Directory of Open Access Journals (Sweden)

    Hai-Jeon Yoon

    Full Text Available This study investigated the relationships between background intestinal uptake on 18F-FDG PET and cardio-metabolic risk (CMR factors.A total of 326 female patients that underwent 18F-FDG PET to determine the initial stage of breast cancer were enrolled. None of the patients had history of diabetes or hypertension. The background intestinal uptake on PET was visually graded (low vs. high uptake group and quantitatively measured using the maximal standardized uptake value (SUVmax. SUVmax of 7 bowel segments (duodenum, jejunum, ileum, cecum, hepatic flexure, splenic flexure, and descending colon-sigmoid junction were averaged for the total bowel (TB SUVmax. Age, body mass index (BMI, fasting blood glucose level (BST, triglyceride (TG, cholesterol, high density lipoprotein (HDL, and low density lipoprotein (LDL were the considered CMR factors. The relationships between background intestinal 18F-FDG uptake on PET and diverse CMR factors were analyzed.The visual grades based on background intestinal 18F-FDG uptake classified 100 (30.7% patients into the low uptake group, while 226 (69.3% were classified into the high uptake group. Among CMR factors, age (p = 0.004, BMI (p<0.001, and TG (p<0.001 were significantly different according to visual grade of background intestinal 18F-FDG uptake. Quantitative TB SUVmax showed significant positive correlation with age (r = 0.203, p<0.001, BMI (r = 0.373, p<0.001, TG (r = 0.338, p<0.001, cholesterol (r = 0.148, p = 0.008, and LDL (r = 0.143, p = 0.024 and significant negative correlation with HDL (r = -0.147, p = 0.022. Multivariate analysis indicated that BMI and TG were independent factors in both visually graded background intestinal 18F-FDG uptake (p = 0.027 and p = 0.023, respectively and quantitatively measured TB SUVmax (p = 0.006 and p = 0.004, respectively.Increased background intestinal 18F-FDG uptake on PET may suggest alteration of lipid metabolism and risk of cardio-metabolic disease in non

  8. How the multiple antioxidant properties of ascorbic acid affect lipid oxidation in oil-in-water emulsions.

    Science.gov (United States)

    Uluata, Sibel; McClements, D Julian; Decker, Eric A

    2015-02-18

    Lipid oxidation is a serious problem for oil-containing food products because it negatively affects shelf life and nutritional composition. An antioxidant strategy commonly employed to prevent or delay oxidation in foods is to remove oxygen from the closed food-packaging system. An alternative technique is use of an edible oxygen scavenger to remove oxygen within the food. Ascorbic acid (AA) is a particularly promising antioxidant because of its natural label and multiple antioxidative functions. In this study, AA was tested as an oxygen scavenger in buffer and an oil-in-water (O/W) emulsion. The effects of transition metals on the ability of AA to scavenge oxygen were determined. Headspace oxygen decrease less than 1% in the medium-chain triacylglycerol (MCT) O/W emulsion system (pH 3 and 7). AA was able to almost completely remove dissolved oxygen (DO) in a buffered solution. Transition metals (Fe(2+) and Cu(+)) significantly accelerated the degradation of AA; however, iron and copper only increased DO depletion rates, by 10.6-16.4% from day 1 to 7, compared to the control. AA (2.5-20 mM) decreased DO in a 1% O/W emulsion system 32.0-64.0% and delayed the formation of headspace hexanal in the emulsion from 7 to over 20 days. This research shows that, when AA is used in an O/W emulsion system, oxidation of the emulsion system can be delay by multiple mechanisms. PMID:25650525

  9. Serum Malondialdehyde and Lipid Profile Levels of Young Patients "Haven’t a Family History of Hypertension": A New Study for Cases in the Civic Society

    Directory of Open Access Journals (Sweden)

    *R. H. Jasim

    2011-09-01

    Full Text Available This study was designed to evaluate the effect of the oxidative stress in sera of young patients without a family history of hypertension, then find the relation of it to lipid profile. The study involved 56 young healthy (30.5 ± 4.7 years and 23 healthy elderly volunteers (66.3 ± 3.5 years, these individuals were compared with 67 patients (28.6 ± 5.0 years, attending the Gastro Intestinal and Liver Centre at Al Sader Medical City and several specialized clinics in Najaf government. Malondialdehyde level was measured by TBARS assay as reflection to the oxidative stress effect. Using standard enzymatic assays, TG, TC, HDL-C, VLDL- -C, and LDL-C values were measured for patients and controls on the same day of sample obtainment. Levels of serum MDA, TG, VLDL-C, and LDL-C were significantly raised (p<0.005 in hypertensive patients group when compared with young and elderly controls, while non significant variations were obtained when the control groups were compared together. Daytime SBP and DBP were both strong positive correlated (r=0.82, p<0.005 for SBP, and r=0.95, p<0.005 for DBP with the MDA in hypertensive patients, but in elderly controls only the correlation between SBP and MDA levels was statistically significant (r=0.61, p<0.005. Sera TG, VLDL-C, and LDL-C levels showed the same of MDA results when they were correlated to SBP and DBP, while correlations between blood pressure with sera TC were moderate positively in the study groups. In spite of; HDL-C levels in hypertensive patients were within the levels of those of two control groups, HDL-C levels showed negatively correlation with both SBP and DBP. Notably, there was a positive correlation (r=0.60, p<0.005 between serum LDL-C levels and SBP of elderly controls, no such correlations were observed when the relation was between Daytime SBP and DBP and VLDL-C or LDL-C of young controls the highly significant positive correlation (r = 0.94 at p<0.005 of MDA and TG of the hypertensive

  10. Evaluation of the Effects of Vaccinium arctostaphylos L. Fruit Extract on Serum Lipids and hs-CRP Levels and Oxidative Stress in Adult Patients with Hyperlipidemia: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

    OpenAIRE

    2014-01-01

    Background. Dyslipidemia produces atherosclerosis, which in turn results in coronary artery disease (CAD). Atherosclerosis is being considered as an inflammatory disease. Vaccinium arctostaphylos L. is a plant with fruits rich in anthocyanins. The aim of this study was to evaluate the effects of fruit extract of this plant on serum levels of lipids, hs-CRP, and malondialdehyde (MDA) as a marker of oxidative stress, in hyperlipidemic adult patients. Methods. In this randomized, double-blind, p...

  11. The effect of whole grain wheat sourdough bread consumption on serum lipids in healthy normoglycemic/normoinsulinemic and hyperglycemic/hyperinsulinemic adults depends on presence of the APOE E3/E3 genotype: a randomized controlled trial

    OpenAIRE

    Bakovic Marica; Graham Terry E; Robinson Lindsay E; MacKay Kathryn A; Tucker Amy J; Duncan Alison M

    2010-01-01

    Abstract Background Epidemiological studies associate consumption of whole grain foods, including breads, with reduced cardiovascular disease (CVD) risk; however, few studies have compared wheat whole grains with wheat refined grains. Methods This study investigated effects of 6-week consumption of whole grain wheat sourdough bread in comparison to white bread on fasting serum lipids in normoglycemic/normoinsulinemic (NGI; n = 14) and hyperglycemic/hyperinsulinemic (HGI; n = 14) adults. The i...

  12. Effect of Lipid Partitioning on Predictions of Acute Toxicity of Oil Sands Process Affected Water to Embryos of Fathead Minnow (Pimephales promelas).

    Science.gov (United States)

    Morandi, Garrett D; Zhang, Kun; Wiseman, Steve B; Pereira, Alberto Dos Santos; Martin, Jonathan W; Giesy, John P

    2016-08-16

    Dissolved organic compounds in oil sands process affected water (OSPW) are known to be responsible for most of its toxicity to aquatic organisms, but the complexity of this mixture prevents use of traditional bottom-up approaches for predicting toxicities of mixtures. Therefore, a top-down approach to predict toxicity of the dissolved organic fraction of OSPW was developed and tested. Accurate masses (i.e., m/z) determined by ultrahigh resolution mass spectrometry in negative and positive ionization modes were used to assign empirical chemical formulas to each chemical species in the mixture. For each chemical species, a predictive measure of lipid accumulation was estimated by stir-bar sorptive extraction (SBSE) to poly(dimethyl)siloxane, or by partitioning to solid-supported lipid membranes (SSLM). A narcosis mode of action was assumed and the target-lipid model was used to estimate potencies of mixtures by assuming strict additivity. A model developed using a combination of the SBSE and SSLM lipid partitioning estimates, whereby the accumulation of chemicals to neutral and polar lipids was explicitly considered, was best for predicting empirical values of LC50 in 96-h acute toxicity tests with embryos of fathead minnow (Pimephales promelas). Model predictions were within 4-fold of observed toxicity for 75% of OSPW samples, and within 8.5-fold for all samples tested, which is comparable to the range of interlaboratory variability for in vivo toxicity testing. PMID:27420640

  13. Methylmalonic and propionic acidemias: lipid profiles of normal and affected human skin fibroblasts incubated with [1-14C]propionate

    International Nuclear Information System (INIS)

    Normal human skin fibroblasts and those from methylmalonic acidemia and propionic acidemia patients were grown in culture. Following incubation with [1-14C]propionate, the major lipid classes in the cells were separated by thin layer chromatography and isolated fractions analyzed by radio gas chromatography for the presence of odd-numbered long-chain fatty acids; the pattern of even-numbered long-chain fatty acids was obtained also. Normal fibroblasts incorporated a small percentage of propionate into odd-numbered fatty acids which were present in all lipids studied. The abnormal cells incorporated a larger amount while maintaining the characteristic ratios of odd-numbered fatty acids found in the normal line. Most of the radioactivity was associated with phospholipids which are the predominant constituents of cell membranes. A characteristic C15/C17 ratio was found for different phospholipids and the triglyceride fraction; pentadecanoic acid was the principal odd-numbered fatty acid utilized in the assembly of complex lipids. Compared to even-numbered long-chain fatty acids the absolute amount of odd-numbered fatty acids was low (1-2%), even in affected cells. An unusual polar lipid fraction was isolated in the course of the study. In the normal cell it contained several unlabeled eicosanoids which were missing from the same fraction of both affected cell lines

  14. Research on correlation between primary hypothyroidism and serum lipid metabolism%原发性甲状腺功能减退症与血脂代谢的相关性研究

    Institute of Scientific and Technical Information of China (English)

    李京丽; 秦丹丹; 李昭英

    2013-01-01

    ObjectiveTo explore the characteristic of serum lipids metabolism under different degrees of hypothyroidism and course, the study observed the dynamic changes in serum lipids metabolism after hormone replacement therapy in patients with hypothyroidism. Methods97 cases of patients with hypothyroidism were collected, 32 patients with subclinical hypothyroidism, 28 patients with moderate hypothyroidism, 37 cases with severe hypothyroidism, and 31 cases of healthy. All patients were examined before and one month after hormone replacement therapy, testing serum free triiodothyronine three (FT3), free thyroxine (FT4), thyroid stimulating hormone (TSH), total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL-C), low density lipoprotein (LDL-C), apolipoprotein A(ApoA), apolipoprotein B(ApoB) and lipoprotein (a)Lp(a). Results Serum lipids of the severe hypothyroidism group were higher than the control group. TC, LDL-C, ApoA, ApoB and Lp(a) of the moderate hypothyroidism group were higher than the control group. ApoA and Lp(a) of the moderate hypothyroidism group were higher than the control group. The serum lipids were negatively related to FT3 and FT4, and TSH was positively correlated. Different degrees of hypothyroidism had different effects on serum lipids. The more increase course of hypothyroidism, the more obvious increase in the serum lipids level. Hormone replacement therapy in patients with hypothyroidism, the serum lipids returned to be normal after one month except for Lp(a).ConclusionHypothyroidism can increase serum lipids. The more increase degree and course of hypothyroidism, the more obvious increase in serum lipids level. With the hormone replacement therapy, the serum lipids resumed with hypothyroidism condition improved gradually, serum lipids returned to be normal before the thyroid function.%目的:探讨不同程度甲状腺功能减退症和病程对血脂的影响,并观察甲状腺功能减退症患者激素替代治疗后血脂

  15. Disruption of the lipid-transporting LdMT-LdRos3 complex in Leishmania donovani affects membrane lipid asymmetry but not host cell invasion.

    Directory of Open Access Journals (Sweden)

    Adrien Weingärtner

    Full Text Available Maintenance and regulation of the asymmetric lipid distribution across eukaryotic plasma membranes is governed by the concerted action of specific membrane proteins controlling lipid movement across the bilayer. Here, we show that the miltefosine transporter (LdMT, a member of the P4-ATPase subfamily in Leishmania donovani, and the Cdc50-like protein LdRos3 form a stable complex that plays an essential role in maintaining phospholipid asymmetry in the parasite plasma membrane. Loss of either LdMT or LdRos3 abolishes ATP-dependent transport of NBD-labelled phosphatidylethanolamine (PE and phosphatidylcholine from the outer to the inner plasma membrane leaflet and results in an increased cell surface exposure of endogenous PE. We also find that promastigotes of L. donovani lack any detectable amount of phosphatidylserine (PS but retain their infectivity in THP-1-derived macrophages. Likewise, infectivity was unchanged for parasites without LdMT-LdRos3 complexes. We conclude that exposure of PS and PE to the exoplasmic leaflet is not crucial for the infectivity of L. donovani promastigotes.

  16. Disruption of the lipid-transporting LdMT-LdRos3 complex in Leishmania donovani affects membrane lipid asymmetry but not host cell invasion.

    Science.gov (United States)

    Weingärtner, Adrien; Drobot, Björn; Herrmann, Andreas; Sánchez-Cañete, María P; Gamarro, Francisco; Castanys, Santiago; Günther Pomorski, Thomas

    2010-01-01

    Maintenance and regulation of the asymmetric lipid distribution across eukaryotic plasma membranes is governed by the concerted action of specific membrane proteins controlling lipid movement across the bilayer. Here, we show that the miltefosine transporter (LdMT), a member of the P4-ATPase subfamily in Leishmania donovani, and the Cdc50-like protein LdRos3 form a stable complex that plays an essential role in maintaining phospholipid asymmetry in the parasite plasma membrane. Loss of either LdMT or LdRos3 abolishes ATP-dependent transport of NBD-labelled phosphatidylethanolamine (PE) and phosphatidylcholine from the outer to the inner plasma membrane leaflet and results in an increased cell surface exposure of endogenous PE. We also find that promastigotes of L. donovani lack any detectable amount of phosphatidylserine (PS) but retain their infectivity in THP-1-derived macrophages. Likewise, infectivity was unchanged for parasites without LdMT-LdRos3 complexes. We conclude that exposure of PS and PE to the exoplasmic leaflet is not crucial for the infectivity of L. donovani promastigotes. PMID:20865154

  17. Effect of Turmeric and Carrot Seed Extracts on Serum Liver Biomarkers and Hepatic Lipid Peroxidation, Antioxidant Enzymes and Total Antioxidant Status in Rats

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    Adel Rezaei-Moghadam

    2012-07-01

    Full Text Available Introduction: Pathogenic role of free radicals are well known in various metabolic diseases. They originate from internal and external sources of body. Essential roles of antioxidant defense system for cellular redox regulation and free radical scavenging activity were described in this study. Many in vitro investigations have shown that turmeric (TE and carrot seed extract (CSE exhibits to possess antioxidant activities. In this study, we evaluated the antioxidant potentials of ethanolic TE and CSE based on in vivo experiment in the rats. Methods: Animals were assigned to six groups: the 1st and 2nd groups were control groups and 2nd group received 0.2 ml dimethyl sulphoxide as vehicle treated group; other four experimental groups received different doses of TE (100, 200 mg/kg b.w. and CSE (200, 400 mg/kg b.w. by gavages, respectively for a period of one month. The indicators of oxidative stress, lipids peroxidation, markers of hepatocyte injury and biliary function markers were measured. Results: The levels of superoxide dismutase, catalase, and glutathione peroxidase were significantly stimulated in the hepatic tissue of treatment groups. The malondialdehyde contents of liver tissue were significantly reduced in the groups fed with TE and CSE. Serum levels of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase, in treated groups were found to be significantly decreased, whereas albumin and total protein increased as compared to the control groups (P<0.05. Conclusion: this study showed that the regular intake of TE and CSE through the diet can improve antioxidant status and inhibit peroxidation activity in the liver tissue so that using these extracts may protect tissue oxidative stress.

  18. Dietary Lipid and Carbohydrate Interactions: Implications on Lipid and Glucose Absorption, Transport in Gilthead Sea Bream (Sparus aurata) Juveniles.

    Science.gov (United States)

    Castro, Carolina; Corraze, Geneviève; Basto, Ana; Larroquet, Laurence; Panserat, Stéphane; Oliva-Teles, Aires

    2016-06-01

    A digestibility trial was performed with gilthead sea bream juveniles (IBW = 72 g) fed four diets differing in lipid source (fish oil, FO; or a blend of vegetable oil, VO) and starch content (0 %, CH-; or 20 %, CH+) to evaluate the potential interactive effects between carbohydrates and VO on the processes involved in digestion, absorption and transport of lipids and glucose. In fish fed VO diets a decrease in lipid digestibility and in cholesterol (C), High Density Lipoprotein(HDL)-C and Low Density Lipoprotein (LDL)-C (only in CH+ group) were recorded. Contrarily, dietary starch induced postprandial hyperglycemia and time related alterations on serum triacylglycerol (TAG), phospholipid (PL) and C concentrations. Fish fed a CH+ diet presented lower serum TAG than CH- group at 6 h post-feeding, and the reverse was observed at 12 h post-feeding for TAG and PL. Lower serum C and PL at 6 h post-feeding were recorded only in VOCH+ group. No differences between groups were observed in hepatic and intestinal transcript levels of proteins involved in lipid transport and hydrolysis (FABP, DGAT, GPAT, MTP, LPL, LCAT). Lower transcript levels of proteins related to lipid transport (ApoB, ApoA1, FABP2) were observed in the intestine of fish fed the CH+ diet, but remained unchanged in the liver. Overall, transcriptional mechanisms involved in lipid transport and absorption were not linked to changes in lipid serum and digestibility. Dietary starch affected lipid absorption and transport, probably due to a delay in lipid absorption. This study suggests that a combination of dietary VO and starch may negatively affect cholesterol absorption and transport. PMID:27023202

  19. Serum lipid profiles of Chinese men with chronic paraplegia%青壮年男性截瘫患者血脂代谢的观察

    Institute of Scientific and Technical Information of China (English)

    雒生杰; 李建军; 李寿霖; 孟申; 周红俊; 杨祖福

    2011-01-01

    Objective To investigate the serum lipids levels of Chinese men with chronic paraplegia.Methods A total of 650 male paraplegics admitted to the China Rehabilitation Research Center (CRRC) for rehabilitation therapy from Jan 2004-Dec 2008 were investigated. The inclusion criteria were a definite paraplegic history (T2-L5 spinal cord injury); complete data; age 18-60 years; and no history of stroke. TG, TC, LDL and HDL serum lipid profiles were studied. Results These male paraplegics showed significantly higher mean TG and TC levels and lower mean HDL levels than the nation-wide averages for similar males ( TG 1.72 mmol/L vs. 1.36 mmol/L; TC4.42 mmol/L vs. 4.30 mmoL/L; HDL 0.99 mmol/L vs. 1.18 mmol/L). No difference in average LDL levels was found. TG, TC and LDL levels were correlated positively with age, but HDL was not. Duration of paraplegia correlated positively with HDL levels, but negatively with LDL. The prevalence of dyslipidemia was 67% , and the prevalence of low HDL was 64%. Conclusions The serum lipid profiles of the young male paraplegics showed a high prevalence of dislipidemia, significantly low HDL levels with a high prevalence of low HDL, and no correlation with age. TG, TC and LDL levels increased with age. The duration of paraplegia was correlated positively with HDL but negatively with LDL, probably due to increased exercise of the upper limbs.%目的 通过流行病学调查来了解青壮年男性截瘫患者血脂代谢水平特点,探讨双下肢运动功能缺失对男性血脂的影响.方法 对中国康复研究中心2004年至2008年因截瘫入院进行康复治疗的男性患者进行入院状态调查.人选要求:①病史明确(T2~L5平面脊髓损伤致双下肢瘫痪);②资料完整;③18周岁≤年龄≤60岁;④非脑卒中患者.共650例患者入选,对血脂指标[甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)]进行分析.结果 青壮年男性截瘫患者TG、TC水平显著高

  20. Alterations of lipid metabolism in Wilson disease

    Directory of Open Access Journals (Sweden)

    Stremmel Wolfgang

    2011-05-01

    Full Text Available Abstract Introduction Wilson disease (WD is an inherited disorder of human copper metabolism, characterised by accumulation of copper predominantly in the liver and brain, leading to severe hepatic and neurological disease. Interesting findings in animal models of WD (Atp7b-/- and LEC rats showed altered lipid metabolism with a decrease in the amount of triglycerides and cholesterol in the serum. However, serum lipid profile has not been investigated in large human WD patient cohorts to date. Patients and Methods This cohort study involved 251 patients examined at the Heidelberg and Dresden (Germany University Hospitals. Patients were analysed on routine follow-up examinations for serum lipid profile, including triglycerides, cholesterol, high density lipoprotein (HDL and low density lipoprotein (LDL. Data on these parameters at time of diagnosis were retrieved by chart review where available. For statistical testing, patients were subgrouped by sex, manifestation (hepatic, neurological, mixed and asymptomatic and treatment (D-penicillamine, trientine, zinc or combination. Results A significant difference in total serum cholesterol was found in patients with hepatic symptoms, which diminished under therapy. No alterations were observed for HDL, LDL and triglycerides. Conclusion Contradictory to previous reports using WD animal models (Atp7b-/- and LEC rats, the most obvious alteration in our cohort was a lower serum cholesterol level in hepatic-affected patients, which might be related to liver injury. Our data suggested unimpaired cholesterol metabolism in Wilson disease under therapy, independent of the applied medical treatment.

  1. The effect of whole grain wheat sourdough bread consumption on serum lipids in healthy normoglycemic/normoinsulinemic and hyperglycemic/hyperinsulinemic adults depends on presence of the APOE E3/E3 genotype: a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Bakovic Marica

    2010-05-01

    Full Text Available Abstract Background Epidemiological studies associate consumption of whole grain foods, including breads, with reduced cardiovascular disease (CVD risk; however, few studies have compared wheat whole grains with wheat refined grains. Methods This study investigated effects of 6-week consumption of whole grain wheat sourdough bread in comparison to white bread on fasting serum lipids in normoglycemic/normoinsulinemic (NGI; n = 14 and hyperglycemic/hyperinsulinemic (HGI; n = 14 adults. The influence of single-nucleotide polymorphisms, 3 within the APOE gene (E2, E3, E4 and 2 within the hepatic lipase gene promoter (LIPC -514C>T, LIPC -250G>A were considered. Results At baseline, HGI participants had significantly higher body weight, waist circumference, body fat, and fasted glucose, insulin, homeostasis model assessment of insulin resistance (HOMA-IR, glucagon, triacylglycerols (TAG and TAG:HDL-cholesterol, compared to NGI participants; however, none of these in addition to none of the other serum lipids, differed between bread treatments, within either participant group. For participants with the APOE E3/E3 genotype, LDL-cholesterol (P = 0.02 increased in the NGI group (n = 7, and TAG (P = 0.03 and TAG:HDL-cholesterol (P = 0.04 increased in the HGI group (n = 10, following consumption of whole grain wheat sourdough compared to white bread. Conclusions In summary, 6-week consumption of whole grain wheat sourdough bread did not significantly modulate serum lipids in NGI or HGI adults; however, it significantly increased LDL-cholesterol, TAG and TAG:HDL-cholesterol in participants with the APOE E3/E3 genotype. These data add to limited literature comparing wheat whole grains to wheat refined grains on CVD risk and highlight the need to consider genetic variation in relation to lipoprotein lipid content and CVD risk.

  2. Serum levels of soluble CD30 in adult patients affected by atopic dermatitis and its relation to age, duration of disease and Scoring Atopic Dermatitis index

    Directory of Open Access Journals (Sweden)

    Gabriele Di Lorenzo

    2003-01-01

    Full Text Available The value of CD30 and the soluble circulating fragment of CD30 (sCD30 for atopic dermatitis (AD remains unclear. In particular, little is known about the effects of age, duration of disease and Scoring Atopic Dermatitis index (SCORAD on the levels of serum sCD30 in patients affected by AD. In the present study, we have analysed serum sCD30 levels of adult patients affected by AD. The study's population includes 18 non-smoking outpatients, with a diagnosis of AD. As a control group we studied 18 non-atopic subjects from laboratory staff, matched for sex and age. These subjects had no history of AD, urticaria or seasonal or perennial rhinitis or asthma, and had negative skin prick test to a panel of allergens.

  3. Distinguishing disease effects from environmental effects in a mountain ungulate: seasonal variation in body weight, hematology, and serum chemistry among Iberian ibex (Capra pyrenaica) affected by sarcoptic mange.

    Science.gov (United States)

    Pérez, Jesús M; Serrano, Emmanuel; Soriguer, Ramón C; González, Francisco J; Sarasa, Mathieu; Granados, José E; Cano-Manuel, Francisco J; Cuenca, Rafaela; Fandos, Paulino

    2015-01-01

    Our study focuses on the Iberian ibex (Capra pyrenaica) from the Sierra Nevada Natural Space (southern Spain), where sarcoptic mange is an endemic disease and animals are affected by a highly seasonal environment. Our aim was to distinguish between disease and environmental influences on seasonal variation in body weight, hematology, and serum biochemistry in Iberian ibex. We sampled 136 chemically immobilized male ibexes. The single effect of mange influenced hemoglobin, hematocrit, mean corpuscular volume, leukocytes, band neutrophils, monocytes, cholesterol, urea, creatine, and aspartate aminotransferase. Both mange and the period of the year also affected values of mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, neutrophils, glucose, and serum proteins. Scabietic animals showed a marked reduction in body weight (21.4 kg on average), which was more pronounced in winter. These results reveal that 1) infested animals are anemic, 2) secondary infections likely occur, and 3) sarcoptic mange is catabolic. PMID:25380360

  4. Trends in the Levels of Serum Lipids and Lipoproteins and the Prevalence of Dyslipidemia in Adults with Newly Diagnosed Type 2 Diabetes in the Southwest Chinese Han Population during 2003–2012

    Directory of Open Access Journals (Sweden)

    Jing Tian

    2015-01-01

    Full Text Available Objective. To determine the trends of serum lipid levels and dyslipidemia in adults newly diagnosed with type 2 diabetes mellitus during 2003–2012 in Southwest China. Methods. Serum lipid measurements of 994 adults were obtained from 5 independent, cross-sectional studies (2003-2004, 2005-2006, 2007-2008, 2009-2010, and 2011-2012. The main outcome measures were mean serum total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and triglyceride levels; body mass index; hemoglobin A1C level; and the percentages of patients with dyslipidemia, hypertension, coronary heart disease, and cerebrovascular disease. Results. The mean total cholesterol and low-density lipoprotein cholesterol levels increased from 4.92 ± 1.15 to 5.30 ± 1.17 mmol/L (P = 0.039 and 2.72 ± 0.83 to 3.11 ± 1.09 mmol/L (P = 0.004, respectively, and the mean HDL cholesterol level declined from 1.22 ± 0.30 to 1.06 ± 0.24 mmol/L (P < 0.001. The percentages of patients with dyslipidemia increased gradually. The incidence of coronary heart and cerebrovascular diseases increased from 8.2% to 19.1% and 6.6% to 15.3%, respectively (P < 0.05. Conclusion. Unfavorable upward trends were observed in serum lipid levels and the prevalence of dyslipidemia, coronary heart disease, and cerebrovascular disease in adults newly diagnosed with type 2 diabetes mellitus in Southwest China during 2003–2012.

  5. Effects of different fibre sources and fat addition on cholesterol and cholesterol-related lipids in blood serum, bile and body tissues of growing pigs.

    Science.gov (United States)

    Kreuzer, M; Hanneken, H; Wittmann, M; Gerdemann, M M; Machmuller, A

    2002-04-01

    Knowledge is limited on the efficacy of hindgut-fermentable dietary fibre to reduce blood, bile and body tissue cholesterol levels. In three experiments with growing pigs the effects of different kinds and levels of bacterially fermentable fibre (BFS) on cholesterol metabolism were examined. Various diets calculated to have similar contents of metabolizable energy were supplied for complete fattening periods. In the first experiment, a stepwise increase from 12 to 20% BFS was performed by supplementing diets with fermentable fibre from sugar beet pulp (modelling hemicelluloses and pectin). Beet pulp, rye bran (modelling cellulose) and citrus pulp (pectin) were offered either independently or in a mixture in the second experiment. These diets were opposed to rations characterized in carbohydrate type by starch either mostly non-resistant (cassava) or partly resistant (maize) to small intestinal digestion. The third experiment was planned to explore the interactions of BFS from citrus pulp with fat either through additional coconut oil/palm kernel oil blend or full-fat soybeans. In all experiments the increase of the BFS content was associated with a constant (cellulose) or decreasing (hemicelluloses, pectin) dietary proportion of non-digestible fibre. In experiment 1 an inverse dose-response relationship between BFS content and cholesterol in blood serum and adipose tissue as well as bile acid concentration in bile was noted while muscle cholesterol did not respond. In experiment 2 the ingredients characterized by cellulose and hemicelluloses/pectin reduced cholesterol-related traits relative to the low-BFS-high-starch controls whereas, except in adipose tissue cholesterol content, the pectinous ingredient had the opposite effect. However, the changes in serum cholesterol mainly affected HDL and not LDL cholesterol. Adipose tissue cholesterol also was slightly lower with partly resistant starch compared to non-resistant starch in the diet. Experiment 3 showed that

  6. Supplemental leucine and isoleucine affect expression of cationic amino acid transporters and myosin, serum concentration of amino acids, and growth performance of pigs.

    Science.gov (United States)

    Cervantes-Ramírez, M; Mendez-Trujillo, V; Araiza-Piña, B A; Barrera-Silva, M A; González-Mendoza, D; Morales-Trejo, A

    2013-01-01

    Leucine (Leu) participates in the activity of cationic amino acid (aa) transporters. Also, branched-chain aa [Leu, isoleucine (Ile), and valine (Val)] share intestinal transporters for absorption. We conducted an experiment with 16 young pigs (body weight of about 16 kg) to determine whether Leu and Ile affect expression of aa transporters b(0,+) and CAT-1 in the jejunum and expression of myosin in muscle, as well as serum concentration of essential aa, and growth performance in pigs. Dietary treatments were: wheat-based diets fortified with Lys, Thr, and Met; basal diet plus 0.50% Leu; basal diet plus 0.50% Ile, and basal diet plus 0.50% Leu and 0.50% Ile. After 28 days, the pigs were sacrificed to collect blood, jejunum, and semitendinosus and longissimus muscle samples. The effects of single and combined addition of Leu and Ile were analyzed. Leu alone or combined with Ile significantly decreased daily weight gain and reduced feed conversion. Leu and Ile, alone or in combination, significantly decreased expression of b(0,+) and significantly increased CAT-1. Ile alone or combined with Leu significantly decreased myosin expression in semitendinosus and significantly decreased it in longissimus muscle. Leu alone significantly decreased Lys, Ile and Thr serum concentrations; Ile significantly decreased Thr serum concentration; combined Leu and Ile significantly decreased Thr and significantly increased Val serum concentration. We conclude that dietary levels of Leu and Ile affect growth performance, expression of aa transporters and myosin, and aa serum concentrations in pigs. PMID:23408397

  7. Amylose content in rice (Oryza sativa affects performance, glycemic and lipidic metabolism in rats Teor de amilose do arroz (Oryza sativa afeta o desempenho, metabolismo glicêmico e lipídico em ratos

    Directory of Open Access Journals (Sweden)

    Cristiane Casagrande Denardin

    2012-02-01

    Full Text Available This research aimed at evaluating the effect of diets with high, intermediate and low amylose content of rice on performance, glycemic and lipidic metabolism in rats. Male Wistar rats were fed diets with grains of cooked rice of the cultivars 'IRGA 417', 'IRGA 416' and 'MOCHI' with high, intermediate and low amylose content, respectively. Wet and dry fecal production and serum HDL cholesterol were not affected by amylose content. The animals in the treatments with high amylose content ('IRGA 417' presented lower feed intake, body weight gain and apparent digestibility, higher fecal water content and nitrogen excretion, reduced fecal pH, lower postprandial blood glucose response, serum total cholesterol and triglycerides levels and pancreas weight, and higher fasting serum glucose concentration and liver weight. Amylose:amylopectin ratio significantly affects rice starch digestion in the gastrointestinal tract, affecting some biologically relevant parameters.O objetivo deste trabalho foi avaliar o efeito de dietas com alto, intermediário e baixo teor de amilose sobre o desempenho, metabolismo glicêmico e lipídico em ratos. Foram utilizados ratos machos Wistar alimentados com rações experimentais elaboradas com grãos de arroz cozido das cultivares 'IRGA 417', 'IRGA 416' e 'MOCHI' com alto, intermediário e baixo teores de amilose, respectivamente. A produção de fezes úmidas e secas e colesterol HDL não foram afetados pelo teor de amilose dos grãos. Os animais submetidos ao tratamento com alto teor de amilose (IRGA 417 apresentaram menores consumo, ganho de peso e digestibilidade aparente, maiores umidade nas fezes e excreção de nitrogênio, reduzido pH fecal, concentração plasmática posprandial de glicose, colesterol total, triglicerídeos e peso do pâncreas e maior concentração de glicose no jejum e peso do fígado. A proporção amilose e amilopectina nos grãos afeta significativamente a digestão do amido de arroz no trato

  8. JAK and STAT members of yellow catfish Pelteobagrus fulvidraco and their roles in leptin affecting lipid metabolism.

    Science.gov (United States)

    Wu, Kun; Tan, Xiao-Ying; Xu, Yi-Huan; Chen, Qi-Liang; Pan, Ya-Xiong

    2016-01-15

    The present study clones and characterizes the full-length cDNA sequences of members in JAK-STAT pathway, explores their mRNA tissue expression and the biological role in leptin influencing lipid metabolism in yellow catfish Pelteobagrus fulvidraco. Full-length cDNA sequences of five JAKs and seven STAT members, including some splicing variants, were obtained from yellow catfish. Compared to mammals, more members of the JAKs and STATs family were found in yellow catfish, which provided evidence that the JAK and STAT family members had arisen by the whole genome duplications during vertebrate evolution. All of these members were widely expressed across the eleven tissues (liver, white muscle, spleen, brain, gill, mesenteric fat, anterior intestine, heart, mid-kidney, testis and ovary) but at the variable levels. Intraperitoneal injection in vivo and incubation in vitro of recombinant human leptin changed triglyceride content and mRNA expression of several JAKs and STATs members, and genes involved in lipid metabolism. AG490, a specific inhibitor of JAK2-STAT pathway, partially reversed leptin-induced effects, indicating that the JAK2a/b-STAT3 pathway exerts main regulating actions of leptin on lipid metabolism at transcriptional level. Meanwhile, the different splicing variants were differentially regulated by leptin incubation. Thus, our data suggest that leptin activated the JAK/STAT pathway and increases the expression of target genes, which partially accounts for the leptin-induced changes in lipid metabolism in yellow catfish. PMID:26704851

  9. Lipid induced insulin resistance affects women less than men and is not accompanied by inflammation or impaired proximal insulin signaling

    DEFF Research Database (Denmark)

    Høeg, Louise D; Sjøberg, Kim Anker; Jeppesen, Jacob;

    2011-01-01

    (60%) after intralipid infusion. Hepatic glucose production was decreased during the clamp similarly in women and men irrespective of intralipid infusion. Intralipid did not impair insulin or AMPK signaling in muscle and subcutaneous fat, did not cause accumulation of muscle lipid intermediates...

  10. Lipid-based nutrient supplements do not affect efavirenz but lower plasma nevirapine concentrations in Ethiopian adult HIV patients

    DEFF Research Database (Denmark)

    Abdissa, A; Olsen, Mette Frahm; Yilma, D; Tesfaye, M; Girma, T; Christiansen, M; Hagen, C M; Wiesner, L; Castel, S; Aseffa, A; McIlleron, H; Pedersen, C; Friis, Henrik; Andersen, A B

    2015-01-01

    OBJECTIVES: Lipid-based nutrient supplements (LNSs) are increasingly used in HIV programmes in resource-limited settings. However, the possible effects of LNSs on the plasma concentrations of antiretroviral drugs have not been assessed. Here, we aimed to assess the effects of LNSs on plasma...

  11. Evaluation of the Effects of Vaccinium arctostaphylos L. Fruit Extract on Serum Lipids and hs-CRP Levels and Oxidative Stress in Adult Patients with Hyperlipidemia: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

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    Rasool Soltani

    2014-01-01

    Full Text Available Background. Dyslipidemia produces atherosclerosis, which in turn results in coronary artery disease (CAD. Atherosclerosis is being considered as an inflammatory disease. Vaccinium arctostaphylos L. is a plant with fruits rich in anthocyanins. The aim of this study was to evaluate the effects of fruit extract of this plant on serum levels of lipids, hs-CRP, and malondialdehyde (MDA as a marker of oxidative stress, in hyperlipidemic adult patients. Methods. In this randomized, double-blind, placebo-controlled clinical trial, 50 hyperlipidemic adult patients were randomly and equally assigned to receive either medicinal (V. arctostaphylos fruit extract or placebo capsules twice daily for 4 weeks. Each medicinal capsule contained 45 ± 2 mg of anthocyanins. Fasting serum levels of total cholesterol, TG, LDL-C, HDL-C, hs-CRP, and MDA were obtained before and after the intervention and compared. Results. V. arctostaphylos fruit extract significantly reduced total cholesterol (P<0.001, LDL-C (P=0.004, TG (P<0.001, and MDA (P=0.013 compared to placebo but did not have any significant effect on HDL-C (P=0.631 and hs-CRP (P=0.190. Conclusion. Fruit extract of Vaccinium arctostaphylos has beneficial effects on serum lipid profile and oxidative stress in hyperlipidemic adult patients. Therefore, it could be considered as a supplement for treatment of dyslipidemia and prevention of atherosclerosis development.

  12. Tamoxifen affects glucose and lipid metabolism parameters, causes browning of subcutaneous adipose tissue and transient body composition changes in C57BL/6NTac mice

    International Nuclear Information System (INIS)

    Tamoxifen is a selective estrogen receptor (ER) modulator which is widely used to generate inducible conditional transgenic mouse models. Activation of ER signaling plays an important role in the regulation of adipose tissue (AT) metabolism. We therefore tested the hypothesis that tamoxifen administration causes changes in AT biology in vivo. 12 weeks old male C57BL/6NTac mice were treated with either tamoxifen (n = 18) or vehicle (n = 18) for 5 consecutive days. Tamoxifen treatment effects on body composition, energy homeostasis, parameters of AT biology, glucose and lipid metabolism were investigated up to an age of 18 weeks. We found that tamoxifen treatment causes: I) significantly increased HbA1c, triglyceride and free fatty acid serum concentrations (p < 0.01), II) browning of subcutaneous AT and increased UCP-1 expression, III) increased AT proliferation marker Ki67 mRNA expression, IV) changes in adipocyte size distribution, and V) transient body composition changes. Tamoxifen may induce changes in body composition, whole body glucose and lipid metabolism and has significant effects on AT biology, which need to be considered when using Tamoxifen as a tool to induce conditional transgenic mouse models. Our data further suggest that tamoxifen-treated wildtype mice should be characterized in parallel to experimental transgenic models to control for tamoxifen administration effects. - Highlights: • Tamoxifen treatment causes significantly increased HbA1c, triglyceride and free fatty acid serum concentrations. • Tamoxifen induces browning of subcutaneous AT and increased UCP-1 expression. • Tamoxifen changes adipocyte size distribution, and transient body composition

  13. Tamoxifen affects glucose and lipid metabolism parameters, causes browning of subcutaneous adipose tissue and transient body composition changes in C57BL/6NTac mice

    Energy Technology Data Exchange (ETDEWEB)

    Hesselbarth, Nico; Pettinelli, Chiara [Department of Medicine, University of Leipzig, D-04103 Leipzig (Germany); Gericke, Martin [Institute of Anatomy, University of Leipzig, D-04103 Leipzig (Germany); Berger, Claudia [IFB Adiposity Disease, Core Unit Animal Models, University of Leipzig, D-04103 Leipzig (Germany); Kunath, Anne [German Center for Diabetes Research (DZD), Leipzig (Germany); Stumvoll, Michael; Blüher, Matthias [Department of Medicine, University of Leipzig, D-04103 Leipzig (Germany); Klöting, Nora, E-mail: nora.kloeting@medizin.uni-leipzig.de [IFB Adiposity Disease, Core Unit Animal Models, University of Leipzig, D-04103 Leipzig (Germany)

    2015-08-28

    Tamoxifen is a selective estrogen receptor (ER) modulator which is widely used to generate inducible conditional transgenic mouse models. Activation of ER signaling plays an important role in the regulation of adipose tissue (AT) metabolism. We therefore tested the hypothesis that tamoxifen administration causes changes in AT biology in vivo. 12 weeks old male C57BL/6NTac mice were treated with either tamoxifen (n = 18) or vehicle (n = 18) for 5 consecutive days. Tamoxifen treatment effects on body composition, energy homeostasis, parameters of AT biology, glucose and lipid metabolism were investigated up to an age of 18 weeks. We found that tamoxifen treatment causes: I) significantly increased HbA{sub 1c}, triglyceride and free fatty acid serum concentrations (p < 0.01), II) browning of subcutaneous AT and increased UCP-1 expression, III) increased AT proliferation marker Ki67 mRNA expression, IV) changes in adipocyte size distribution, and V) transient body composition changes. Tamoxifen may induce changes in body composition, whole body glucose and lipid metabolism and has significant effects on AT biology, which need to be considered when using Tamoxifen as a tool to induce conditional transgenic mouse models. Our data further suggest that tamoxifen-treated wildtype mice should be characterized in parallel to experimental transgenic models to control for tamoxifen administration effects. - Highlights: • Tamoxifen treatment causes significantly increased HbA{sub 1c}, triglyceride and free fatty acid serum concentrations. • Tamoxifen induces browning of subcutaneous AT and increased UCP-1 expression. • Tamoxifen changes adipocyte size distribution, and transient body composition.

  14. Hydrocortisone stimulates the development of oligodendrocytes in primary glial cultures and affects glucose metabolism and lipid synthesis in these cultures

    NARCIS (Netherlands)

    Warringa, R.A.J.; Hoeben, R.C.; Koper, W.J.; Sykes, J.E.C.; Golde, L.M.G. van; Lopes-Cardozo, M.

    1987-01-01

    Cultures of glial cells were prepared from the brains of one-week-old rat pups. After one day in culture, serum was omitted from the medium and replaced by a combination of growth-stimulating hormones and other factors that enhanced the percentage of oligodendrocytes in the cultures. We investigated

  15. Influences of Short -term Aerobic Exercise and Supplementation of Carnitine With or Without Choline on Body Weight, Serum Leptin and Carnitine as Well as Lipid Status In Male Rats

    Directory of Open Access Journals (Sweden)

    Neamat E. Hishem*, Bushra H. El-Zawahry*, Seham M.S. El Nakeeb**

    2006-12-01

    Full Text Available Background: Carnitine is essential for fatty acids translocation, muscles function and exercise performance. Choline is a lipotropic agent that prevents deposition of fat in the liver. The studies concerning the effects of carnitine and choline supplementation with exercise on carnitine status and serum leptin are rare. The aim of the present study was to study the effect of carnitine and its combination with choline, with or without exercise on body and total fat pad (TFP weights, serum carnitine, leptin, -hydroxy butyric acid (-HBA, triacylglycerols (TAG and Free Fatty acids (FFA. Also, total lipids (TL and TAG content of TFP and urinary carnitine were investigated. Material and Methods: 48 male rats were equally divided to the following groups: control (C, carnitine (5 g/Kg diet supplemented, carnitine plus choline (5 and 11.5 g /Kg diet respectively supplemented. Half of each group was subjected to short term aerobic exercise on manual treadmill, in which the speed and duration were gradually increased via the course of the experiment, to be 10 m/min for 20 min/day, 5 days/week in the last 2 weeks. Body weights were recorded weekly. After 6 weeks, The 24 hours urine was collected then the fasted rats were sacrificed and blood and the total fat pad (TFP were collected for analysis. Results: Carnitine supplementation, tended to decrease body weight, TFP, TAG content and serum FFA, and significantly decreased the TL content, serum leptin, TAG (P<0.0005. Carnitine feeding resulted in a significant elevation of serum carnitine, -HBA and urinary carnitine (P<0.0005, compared to sedentary control rats. These values became more pronounced on choline addition to the diet except for serum and urinary carnitine that reversed (i.e. decreased by choline addition. Exercise intervention resulted in a significant decrease in body weight, TFP, TL content and serum leptin, TAG and FFA. These values were more pronounced in both supplements with exercise

  16. Antifungal canthin-6-one series accumulate in lipid droplets and affect fatty acid metabolism in Saccharomyces cerevisiae

    OpenAIRE

    Lagoutte, D.; Nicolas, V; Poupon, E.; Fournet, Anne; Hocquemiller, R.; Libong, D.; Chaminade, P.; Loiseau, P.M.

    2008-01-01

    The mechanism of action of antifungal canthin-6-one series was investigated in Saccharomyces cerevisiae. After a rapid uptake, a preferential accumulation of the drug within lipid droplets was observed. The antifungal action of canthin-6-one was found as reversible. Canthin-6-one did not exhibit affinity for sterols, and membrane ergosterol was not necessary for the antifungal activity since the MICs were similar on an ergosterol-deleted and the wild-type S. cerevisiae clones. Relative amount...

  17. Isolation and Expression Analysis of STAT Members from Synechogobius hasta and Their Roles in Leptin Affecting Lipid Metabolism

    Directory of Open Access Journals (Sweden)

    Kun Wu

    2016-03-01

    Full Text Available Signal transducers and activators of transcription proteins (STATs act as important mediators in multiple biological processes induced by a large number of cytokines. In the present study, full-length cDNA sequences of seven STAT members, including some splicing variants different from those in mammals, were obtained from Synechogobius hasta. The phylogenetic analysis revealed that the seven STAT members were derived from paralogous genes that might have arisen by whole genome duplication (WGD events during vertebrate evolution. All of these members share similar domain structure compared with those of mammals, and were widely expressed across the tested tissues (brain, gill, heart, intestine, liver, muscle and spleen, but at variable levels. Incubation in vitro of recombinant human leptin changed the intracellular triglyceride (TG content and mRNA levels of several STATs members, as well as expressions and activities of genes involved in lipid metabolism. Furthermore, Tyrphostin B42 (AG490, a specific inhibitor of the Janus Kinase 2(JAK2-STAT pathway, partially reversed leptin-induced change on STAT3 and its two spliced isoforms expression, as well as expressions and activities of genes involved in lipid metabolism. As a consequence, the decrease of TG content was also reversed. Thus, our study suggests that STAT3 is the requisite for the leptin signal and the activation of the STAT3 member may account for the leptin-induced changes in lipid metabolism in S. hasta.

  18. Effect of Probiotic Lactobacillus salivarius UBL S22 and Prebiotic Fructo-oligosaccharide on Serum Lipids, Inflammatory Markers, Insulin Sensitivity, and Gut Bacteria in Healthy Young Volunteers: A Randomized Controlled Single-Blind Pilot Study.

    Science.gov (United States)

    Rajkumar, Hemalatha; Kumar, Manoj; Das, Nilita; Kumar, S Nishanth; Challa, Hanumanth R; Nagpal, Ravinder

    2015-05-01

    This study investigated the effect of 6-week supplementation of a probiotic strain Lactobacillus salivarius UBL S22 with or without prebiotic fructo-oligosaccharide (FOS) on serum lipid profiles, immune responses, insulin sensitivity, and gut lactobacilli in 45 healthy young individuals. The patients were divided into 3 groups (15/group), that is, placebo, probiotic, and synbiotic. After 6 weeks, a significant reduction (P FOS was observed to be more beneficial than L salivarius alone, thereby advocating that such synbiotic combinations could be therapeutically exploited for improved health and quality of life. PMID:25331262

  19. Evaluation of the Effects of Vaccinium arctostaphylos L. Fruit Extract on Serum Lipids and hs-CRP Levels and Oxidative Stress in Adult Patients with Hyperlipidemia: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial.

    Science.gov (United States)

    Soltani, Rasool; Hakimi, Mustafa; Asgary, Sedigheh; Ghanadian, Syed Mustafa; Keshvari, Mahtab; Sarrafzadegan, Nizal

    2014-01-01

    Background. Dyslipidemia produces atherosclerosis, which in turn results in coronary artery disease (CAD). Atherosclerosis is being considered as an inflammatory disease. Vaccinium arctostaphylos L. is a plant with fruits rich in anthocyanins. The aim of this study was to evaluate the effects of fruit extract of this plant on serum levels of lipids, hs-CRP, and malondialdehyde (MDA) as a marker of oxidative stress, in hyperlipidemic adult patients. Methods. In this randomized, double-blind, placebo-controlled clinical trial, 50 hyperlipidemic adult patients were randomly and equally assigned to receive either medicinal (V. arctostaphylos fruit extract) or placebo capsules twice daily for 4 weeks. Each medicinal capsule contained 45 ± 2 mg of anthocyanins. Fasting serum levels of total cholesterol, TG, LDL-C, HDL-C, hs-CRP, and MDA were obtained before and after the intervention and compared. Results. V. arctostaphylos fruit extract significantly reduced total cholesterol (P atherosclerosis development. PMID:24587807

  20. Measurement of serum lipids and lipoprotein in cerebral infaraction patients and its clinical significance%脑梗塞患者血脂和脂蛋白的检测及临床意义

    Institute of Scientific and Technical Information of China (English)

    席刚; 刘芳; 侯东哲; 陆彤; 李毅; 张春时

    2001-01-01

    目的:探讨血脂在脑梗塞发病中的作用。方法:检测128例急性脑梗塞患者血脂4项指标含量,并与对照组[摘要]目的:探讨血脂在脑梗塞发病中的作用。方法:检测128例急性脑梗塞患者血脂4项指标含量,并与对照组80例的检测结果进行比较。同时将无合并症脑梗塞患者分别与脑梗塞合并高血压、糖尿病、冠心病患者的血脂水平进行比较。TC,TG及HDL-C等用酶法测定。LDL-C由TC,TG,HDL-C的检测数据按Friedwald公式计算。结果:脑梗塞患者的TC,TG,LDL-C,TC/HDL-C明显高于对照组(P<0.01),而其HDL-C明显低于对照组(P<0.01)。脑梗塞合并高血压、合并糖尿病患者的血脂水平高于无合并症脑梗塞患者,但脑梗塞合并冠心病患者的血脂水平与后者无明显差别。结论:TC,TG,LDL-C,TC/HDL-C的水平升高,HDL-C下降与脑梗塞密切相关,故调脂治疗是非常必要的。%Objective: To investigate the role of serum lipids in the pathogenesis of cerebral infaraction (CI) .Methods: The four indices of serum lipids in 128 patients with CI (patient group) and 80 healthy persons(control group) were measured and compared. The serum lipids of CI without complicated diseases patients were compared with that of CI complicated hypertension, CI complicated diabetes and CI complicated coronary heart diseases patients respectively. Methods: TC, TG and HDL-C were measured by enzyme method. According to Friedwald formular LDL-C could be calculated with measured data of TC, TG, HDL-C. Results:TC, TG, LDL-C and TC/HDL-C of CI patients were significantly higher than those of control group (P<0.01) and their HDL-C was significantly lower than that of the latter(P <0.01). The serum lipid level of CI complicated hipertension and complicated diabetes patients was higher than that of CI without complicated diseases patients. But the serum lipid of CI complicated coronary heart disease

  1. Ripened dairy products differentially affect hepatic lipid content and adipose tissue oxidative stress markers in obese and type 2 diabetic mice.

    Science.gov (United States)

    Geurts, Lucie; Everard, Amandine; le Ruyet, Pascale; Delzenne, Nathalie M; Cani, Patrice D

    2012-02-29

    Growing evidence suggests that the consumption of dairy products may contribute to a reduced incidence of cardiovascular risk factors, such as obesity, dyslipidemia, and type 2 diabetes. The fatty acid composition in milk fat, the duration of ripening, and the complexity of the food matrices are important factors that may interfere with the physiological impact. In this study, we treated genetic obese and type 2 diabetic mice (db/db) for 4 weeks with different dairy (cheese-based) products, differing by the duration of ripening (0, 15, or 35 days). We found that 35 days ripened product significantly improved glucose tolerance, an effect associated with a decreased adipose tissue lipid peroxide markers (TBARS and NAPDH-oxidase mRNA expression), without affecting body weight, food intake, and fat mass. Both fermented matrices significantly decreased the hepatic lipid content, without modifying plasma triglycerides or plasma total cholesterol. These data suggest that dairy products issued from longer ripening positively impact glucose tolerance, hepatic steatosis, and adipose tissue oxidative stress. Further investigations are warranted to decipher the interactions between milk products fermentation, lipids, and host metabolism. PMID:22300436

  2. Zearalenone Affects Immune-Related Parameters in Lymphoid Organs and Serum of Rats Vaccinated with Porcine Parvovirus Vaccine

    OpenAIRE

    Choi, Byung-Kook; Cho, Joon-Hyung; Jeong, Sang-Hee; Shin, Hyo-Sook; Son, Seong-Wan; Yeo, Young-Keun; KANG, HWAN-GOO

    2012-01-01

    Rats were administered zearalenone (ZEA) via gavage at dosages of 0, 1, 5, and 30 mg/kg for 36 days. On treatment day 8, inactivated porcine parvovirus vaccine (Vac) was injected intraperitoneally. Antibody production against porcine parvovirus was then measured as a function of ZEA treatment. Compared to the vaccine alone, ZEA treatment, with or without Vac, decreased the serum level of IgG. The level of IgM decreased in all ZEA groups at day 22, but the decrease was sustained only in the me...

  3. Dietary energy sources affect the partition of body lipids and the hierarchy of energy metabolic pathways in growing pigs differing in feed efficiency

    OpenAIRE

    Gondret, Florence; Louveau, Isabelle; Mourot, Jacques; Duclos, Michel; Lagarrigue, Sandrine; Gilbert, Hélène; Van Milgen, Jacob

    2014-01-01

    The use and partition of feed energy are key elements in productive efficiency of pigs. This study aimed to determine whether dietary energy sources affect the partition of body lipids and tissue biochemical pathways of energy use between pigs differing in feed efficiency. Forty-eight barrows (pure Large White) from two divergent lines selected for residual feed intake (RFI), a measure of feed efficiency, were compared. From 74 d to 132 ± 0.5 d of age, pigs (n = 12 by line and by diet) were o...

  4. Lipid peroxidation in chicken meat during chilled storage as affected by antioxidants combined with low-dose gamma irradiation

    International Nuclear Information System (INIS)

    TBA values and carbonyl content for irradiated samples of ground chicken meat were higher than for nonirradiated samples. Addition of antioxidants tocopherol (natural) or BHT (synthetic) resulted in retardation of oxidative rancidity (p0.05). Meat treated with antioxidants prior to irradiation had lower TBA values as compared to untreated irradiated counterparts. Free fatty acid (FFA) values decreased after irradiation. Addition of antioxidants prior to irradiation showed a synergistic effect in decreasing FFA content. TLC of muscle lipids indicated a reduction in the triacylglcerols content with concomitant increases in FFA of all samples during storage. All irradiated meats were acceptable for consumption up to 4 wk of storage

  5. N-3 Polyunsaturated Fatty Acids Supplementation Does not Affect Changes of Lipid Metabolism Induced in Rats by Altered Thyroid Status

    Czech Academy of Sciences Publication Activity Database

    Rauchová, Hana; Vokurková, Martina; Pavelka, Stanislav; Behuliak, Michal; Tribulová, N.; Soukup, Tomáš

    2013-01-01

    Roč. 45, č. 7 (2013), s. 507-512. ISSN 0018-5043 R&D Projects: GA ČR(CZ) GA303/09/0570; GA ČR(CZ) GA304/08/0256; GA ČR(CZ) GAP304/12/0259; GA MŠk(CZ) 7AMB12SK158 Institutional research plan: CEZ:AV0Z50110509 Institutional support: RVO:67985823 Keywords : hypothyriodism * hyperthyroidism * mitochondrial glycerol-3-phosphate dehydrogenase * glucose * plasma lipids Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery Impact factor: 2.038, year: 2013

  6. High dose flaxseed oil supplementation may affect fasting blood serum glucose management in human type 2 diabetics.

    Science.gov (United States)

    Barre, Douglas E; Mizier-Barre, Kazimiera A; Griscti, Odette; Hafez, Kevin

    2008-01-01

    Type 2 diabetes is characterized partially by elevated fasting blood serum glucose and insulin concentrations and the percentage of hemoglobin as HbA1c. It was hypothesized that each of blood glucose and its co-factors insulin and HbA1c and would show a more favorable profile as the result of flaxseed oil supplementation. Patients were recruited at random from a population pool responding to a recruitment advertisement in the local newspaper and 2 area physicians. Completing the trial were 10 flaxseed oil males, 8 flaxseed oil females, 8 safflower (placebo) oil males and 6 safflower oil females. Patients visited on two pre-treatment occasions each three months apart (visits 1 and 2). At visit 2 subjects were randomly assigned in double blind fashion and in equal gender numbers to take flaxseed oil or safflower oil for three further months until visit 3. Oil consumption in both groups was approximately 10 g/d. ALA intake in the intervention group was approximately 5.5 g/d. Power was 0.80 to see a difference of 1 mmol of glucose /L using 12 subjects per group with a p impact on fasting blood serum glucose, insulin or HbA1c levels. It is concluded that high doses of flaxseed oil have no effect on glycemic control in type 2 diabetics. PMID:18391475

  7. Polymorphic variant at the IL2 region is associated with type 1 diabetes and may affect serum levels of interleukin-2

    OpenAIRE

    Fichna, Marta; Żurawek, Magdalena; Fichna, Piotr; Ziółkowska-Suchanek, Iwona; Januszkiewicz, Danuta; Nowak, Jerzy

    2013-01-01

    Polymorphic variants at the interleukin-2 (IL2) locus affect the risk of several autoimmune disorders. Our aim was to evaluate the association of the four IL2 polymorphisms (rs6822844, rs6534349, rs2069762 and rs3136534) with type 1 diabetes (T1D) in the Polish population, and to correlate them with the serum interleukin-2 levels. 543 unrelated T1D patients and 706 healthy control subjects were enrolled. The minor T allele at rs6822844 was significantly less frequent in T1D compared to contro...

  8. School meals with fish affect serum vitamin d in 8-11 year-old children - preliminary results from the opus school meal study

    DEFF Research Database (Denmark)

    Petersen, R. A.; Damsgaard, C. T.; Dalskov, S.-M.;

    Background and aims Few foods contain vitamin D and many children fail to meet recommended intakes, including Danish children. This may promote low serum concentrations, particularly as cutaneous vitamin D production is negligible during winter/spring at Northern latitudes. Aims To examine if New...... Body Less Head size-adjusted Bone Mineral Content than the control diet, but lower in May/June (p<0.05). No observed effects on Bone Area and Bone Mineral Density. Conclusions Fish-containing NND school meals affected vitamin D intake and ?status, and seemed to mitigate children?s decreases in 25(OH...

  9. Modifications in Lipid Levels Are Independent of Serum TNF-α in Rheumatoid Arthritis: Results of an Observational 24-Week Cohort Study Comparing Patients Receiving Etanercept Plus Methotrexate or Methotrexate as Monotherapy

    Directory of Open Access Journals (Sweden)

    Norma Alejandra Rodriguez-Jimenez

    2014-01-01

    Full Text Available Objective. To compare the modifications in lipids between patients with rheumatoid arthritis (RA receiving etanercept plus methotrexate (ETA + MTX versus methotrexate (MTX and their relationship with serum levels of tumor necrosis factor-alpha (TNF-α. Methods. In an observational cohort study, we compared changes in lipid levels in patients receiving ETA + MTX versus MTX in RA. These groups were assessed at baseline and at 4 and 24 weeks, measuring clinical outcomes, total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-C, low-density lipoprotein cholesterol, and TNF-α. Results. Baseline values for lipid levels were similar in both groups. HDL-C levels increased significantly only in the ETA + MTX group (from 45.5 to 50.0 mg/dL at 4 weeks, a 10.2% increase, P<0.001, and to 56.0 mg/dL at 24 weeks, a 25.1% increase, P<0.001, while other lipids underwent no significant changes. ETA + MTX also exhibited a significant increase in TNF-α (44.8 pg/mL at baseline versus 281.4 pg/mL at 24 weeks, P<0.001. The MTX group had no significant changes in lipids or TNF-α. Significant differences in HDL-C between groups were observed at 24 weeks (P=0.04 and also in TNF-α  (P=0.01. Conclusion. HDL-C levels increased significantly following treatment with ETA + MTX, without a relationship with decrease of TNF-α.

  10. Modifications in Lipid Levels Are Independent of Serum TNF-α in Rheumatoid Arthritis: Results of an Observational 24-Week Cohort Study Comparing Patients Receiving Etanercept Plus Methotrexate or Methotrexate as Monotherapy

    Science.gov (United States)

    Rodriguez-Jimenez, Norma Alejandra; Garcia-Gonzalez, Carlos E.; Ayala-Lopez, Karina Patricia; Trujillo-Hernandez, Benjamin; Aguilar-Chavez, Erika Anita; Rocha-Muñoz, Alberto Daniel; Vasquez-Jimenez, Jose Clemente; Olivas-Flores, Eva; Salazar-Paramo, Mario; Corona-Sanchez, Esther Guadalupe; Vazquez-Del Mercado, Monica; Varon-Villalpando, Evangelina; Cota-Sanchez, Adolfo; Cardona-Muñoz, Ernesto German; Gamez-Nava, Jorge I.; Gonzalez-Lopez, Laura

    2014-01-01

    Objective. To compare the modifications in lipids between patients with rheumatoid arthritis (RA) receiving etanercept plus methotrexate (ETA + MTX) versus methotrexate (MTX) and their relationship with serum levels of tumor necrosis factor-alpha (TNF-α). Methods. In an observational cohort study, we compared changes in lipid levels in patients receiving ETA + MTX versus MTX in RA. These groups were assessed at baseline and at 4 and 24 weeks, measuring clinical outcomes, total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol, and TNF-α. Results. Baseline values for lipid levels were similar in both groups. HDL-C levels increased significantly only in the ETA + MTX group (from 45.5 to 50.0 mg/dL at 4 weeks, a 10.2% increase, P < 0.001, and to 56.0 mg/dL at 24 weeks, a 25.1% increase, P < 0.001), while other lipids underwent no significant changes. ETA + MTX also exhibited a significant increase in TNF-α (44.8 pg/mL at baseline versus 281.4 pg/mL at 24 weeks, P < 0.001). The MTX group had no significant changes in lipids or TNF-α. Significant differences in HDL-C between groups were observed at 24 weeks (P = 0.04) and also in TNF-α  (P = 0.01). Conclusion. HDL-C levels increased significantly following treatment with ETA + MTX, without a relationship with decrease of TNF-α. PMID:25243145

  11. How Do the Size, Charge and Shape of Nanoparticles Affect Amyloid β Aggregation on Brain Lipid Bilayer?

    Science.gov (United States)

    Kim, Yuna; Park, Ji-Hyun; Lee, Hyojin; Nam, Jwa-Min

    2016-01-01

    Here, we studied the effect of the size, shape, and surface charge of Au nanoparticles (AuNPs) on amyloid beta (Aβ) aggregation on a total brain lipid-based supported lipid bilayer (brain SLB), a fluid platform that facilitates Aβ-AuNP aggregation process. We found that larger AuNPs induce large and amorphous aggregates on the brain SLB, whereas smaller AuNPs induce protofibrillar Aβ structures. Positively charged AuNPs were more strongly attracted to Aβ than negatively charged AuNPs, and the stronger interactions between AuNPs and Aβ resulted in fewer β-sheets and more random coil structures. We also compared spherical AuNPs, gold nanorods (AuNRs), and gold nanocubes (AuNCs) to study the effect of nanoparticle shape on Aβ aggregation on the brain SLB. Aβ was preferentially bound to the long axis of AuNRs and fewer fibrils were formed whereas all the facets of AuNCs interacted with Aβ to produce the fibril networks. Finally, it was revealed that different nanostructures induce different cytotoxicity on neuroblastoma cells, and, overall, smaller Aβ aggregates induce higher cytotoxicity. The results offer insight into the roles of NPs and brain SLB in Aβ aggregation on the cell membrane and can facilitate the understanding of Aβ-nanostructure co-aggregation mechanism and tuning Aβ aggregate structures.

  12. Evaluation of the Effects of Pinus koraiensis Needle Extracts on Serum Lipid and Oxidative Stress in Adults with Borderline Dyslipidemia: A Randomized, Double-Blind, and Placebo-Controlled Clinical Trial.

    Science.gov (United States)

    Lee, Hansongyi; Kim, Hyerang; Choue, Ryowon; Lim, Hyunjung

    2016-01-01

    Background. Dyslipidemia has been well-known as a common metabolic disorder contributing to cardiovascular disease. The aim of this study was to evaluate the effect of the Pinus koraiensis needle extracts (PKE) on the blood cholesterol and oxidative stress. Method. We conducted a 12-week randomized, double-blinded controlled trial to examine the effect of PKE on blood lipid profiles in adults with borderline dyslipidemia. Thirty-three eligible persons were recruited and randomly assigned into PKE (n = 20) and placebo groups (n = 13). Serum lipids including total cholesterol, low-density lipoprotein- (LDL-) cholesterol, high-density lipoprotein- (HDL-) cholesterol, very low-density lipoprotein- (VLDL-) cholesterol, and triglyceride were measured before and after trial. Serum insulin, glucose, and antioxidant indicators were also analyzed before and after trial and anthropometry and blood pressure were measured every 4 weeks. Results. After 12 weeks, PKE statically significant decreases in systolic blood pressure (p effective in improving the superoxide dismutase in the individuals with borderline dyslipidemia. PMID:27610187

  13. DIETARY MANGANESE INTAKE AND TYPE OF LIPID DO NOT AFFECT CLINICAL OR NEUROPSYCHOLOGICAL MEASURES IN HEALTHY YOUNG WOMEN

    Science.gov (United States)

    Because manganese (Mn) is potentially toxic, and because dietary fat type may affect Mn absorption, the objectives of the current study were to determine whether diets containing very low or very high amounts of Mn and enriched in either saturated or unsaturated fats affected measures of neuropsycho...

  14. Ascorbyl palmitate, gamma-tocopherol, and EDTA affect lipid oxidation in fish oil enriched salad dressing differently

    DEFF Research Database (Denmark)

    Let, M.B.; Jacobsen, Charlotte; Meyer, Anne S.

    2007-01-01

    The aim of the study was to investigate the ability of γ-tocopherol, ethylenediaminetetraacetate (EDTA), and ascorbyl palmitate to protect fish oil enriched salad dressing against oxidation during a 6 week storage period at room temperature. The lipid-soluble γ-tocopherol (220 and 880 µg g-1 of...... antioxidant, and overall peroxide values and volatiles were reduced by approximately 70 and 77-86%, respectively. Conversely, prooxidant effects were observed with a high concentration of ascorbyl palmitate (300 µg g-1 of fish oil), whereas a low concentration was slightly antioxidative (50 µg/g of fish oil......). Finally, a combination of all three antioxidants completely inhibited oxidation during storage, indicating that the prooxidant effects of ascorbyl palmitate were reverted or overshadowed by EDTA and γ-tocopherol....

  15. Green tea extract suppresses adiposity and affects the expression of lipid metabolism genes in diet-induced obese zebrafish

    Directory of Open Access Journals (Sweden)

    Hasumura Takahiro

    2012-08-01

    Full Text Available Abstract Background Visceral fat accumulation is one of the most important predictors of mortality in obese populations. Administration of green tea extract (GTE can reduce body fat and reduce the risk of obesity-related diseases in mammals. In this study, we investigated the effects and mechanisms of GTE on adiposity in diet-induced obese (DIO zebrafish. Methods Zebrafish at 3.5 to 4.5 months post-fertilization were allocated to four groups: non-DIO, DIO, DIO + 0.0025%GTE, and DIO + 0.0050%GTE. The non-DIO group was fed freshly hatched Artemia once daily (5 mg cysts/fish daily for 40 days. Zebrafish in the three DIO groups were fed freshly hatched Artemia three times daily (60 mg cysts/fish daily. Zebrafish in the DIO + 0.0025%GTE and DIO + 0.0050%GTE groups were exposed to GTE after the start of feeding three times daily for 40 days. Results Three-dimensional microcomputed tomography analysis showed that GTE exposure significantly decreased the volume of visceral but not subcutaneous fat tissue in DIO zebrafish. GTE exposure increased hepatic expression of the lipid catabolism genes ACOX1 (acyl-coenzyme A oxidase 1, palmitoyl, ACADM (acyl-coenzyme A dehydrogenase, c-4 to c-12 straight chain, and PPARA (peroxisome proliferator-activated receptor alpha. GTE exposure also significantly decreased the visceral fat expression of SOCS3 (suppressor of cytokine signaling 3b which inhibits leptin signaling. Conclusions The present results are consistent with those seen in mammals treated with GTE, supporting the validity of studying the effects of GTE in DIO zebrafish. Our results suggest that GTE exerts beneficial effects on adiposity, possibly by altering the expression of lipid catabolism genes and SOCS3.

  16. Efecto de la suplementación de grasas sobre las concentraciones séricas de progesterona, insulina, somatotropina y algunos metabolitos de los lípidos en ovejas Pelibuey Effect of fat supplementation on serum concentrations of progesterone, insulin, growth hormone and some lipid metabolites in Pelibuey ewes

    Directory of Open Access Journals (Sweden)

    JL Espinoza

    2008-01-01

    Full Text Available Se utilizaron ovejas Pelibuey multíparas para determinar la influencia de los jabones de calcio de ácidos grasos (JCAG y de la grasa bovina en la dieta sobre la concentración sérica de insulina, somatotropina, progesterona y metabolitos de los lípidos (colesterol total, lipoproteínas de alta densidad, lipoproteínas de baja densidad y triglicéridos. Las ovejas (n = 6 por grupo se asignaron aleatoriamente a una dieta que contenía 1,5% de JCAG (JC, 1,2% de grasa bovina (S o a una dieta testigo (T sin grasa adicional. Las dietas se proporcionaron durante 75 días. La ganancia de peso fue similar entre tratamientos (P > 0,05. La concentración de metabolitos de lípidos y de progesterona no estuvieron afectadas por la dieta (P > 0,05. La concentración de insulina fue mayor (P 0,05. Se concluye que la grasa bovina y los jabones de calcio de ácidos grasos incorporados en las dietas de ovejas Pelibuey en los tipos y las proporciones señaladas no afectan la ganancia de peso, la concentración de progesterona ni de los metabolitos de los lípidos, pero la grasa de bovino incrementó el nivel sérico de insulina y redujo la somatotropina.In this study, multiparous Pelibuey ewes were used to determine the influence of calcium soap of fatty acids (CSFA and bovine fat in the diet on serum concentrations of insulin, somatotropin, progesterone and lipid metabolites (total cholesterol, high and low density lipoproteins and triglycerides. Ewes (n = 6 per group were assigned randomly to a diet that contained 1.5% CSFA (CS or 1.2% bovine fat (S, or to a control diet (T with no additional fat. The diets were provided during 75 days. Weight gain among treatments was similar (P > 0.05. The concentrations of lipid metabolites and progesterone were not affected by the diet (P > 0.05. Insulin concentration was higher (P 0.05. In conclusion, bovine fat and calcium soap of fatty acids incorporated in the diet of Pelibuey ewes, in the indicated proportions, did

  17. Lipid fatty acid profile analyses in liver and serum in rats with nonalcoholic steatohepatitis using improved gas chromatography-mass spectrometry methodology

    Science.gov (United States)

    Fatty acids (FA) are essential components of lipids and exhibit important biological functions. The analyses of FAs are routinely carried out by gas chromatography-mass spectrometry, after multi-step sample preparation. In this study, several key experimental factors were carefully examined, validat...

  18. Relationship between Pre-Procedural Serum Lipid Profile and Post-Procedural Myocardial Injury in Patients Undergoing Elective Percutaneous Coronary Intervention

    OpenAIRE

    Maadani, Mohsen; Abdi, Seifollah; Parchami-Ghazaee, Sepideh; Alizadeh, Keivan; Fathi, Hosein; Musavi, Reza

    2013-01-01

    Background: Along with technological progress in coronary intervention, periprocedural complications and adverse outcomes have markedly improved, yet perioperative myocardial injury is a frequent complication during percutaneous coronary intervention (PCI) and is strongly associated with post-procedural cardiovascular morbidity and mortality. Epidemiological researchers have defined lipid and lipoproteins abnormality as a risk factor for atherosclerotic cardiovascular diseases. Although sever...

  19. LIPID PEROXIDATION IN PREECLAMPSIA

    Directory of Open Access Journals (Sweden)

    T.Sharmila Krishna

    2015-03-01

    Full Text Available Hypertension in pregnancy is a leading cause of both maternal and fetal mortality and morbidity. Preeclampsia is characterised by hypertension and proteinuria. Lipid peroxidation is an important factor in the pathophysiology of Preeclampsia. The present study was undertaken to determine Serum Malondialdehyde (MDA levels , a product of lipid peroxidation , in clinically diagnosed Preeclamptic women(n=30 and the values were compared with that of Normotensive pregnant women (n=30 aged between 18-30yrs. All of them were in their third trimester and were primigravida. Serum MDA was estimated by TBARS (thiobarbituric acid reactive substances method. We observed that Serum MDA levels were significantly increased in Preeclamptic women (p <0.000 as compared to that of Normotensive pregnant women . Increased levels of lipid peroxiation product - MDA may contribute to the pathophysiology of Preeclampsia.

  20. Dietary energy sources affect the partition of body lipids and the hierarchy of energy metabolic pathways in growing pigs differing in feed efficiency.

    Science.gov (United States)

    Gondret, F; Louveau, I; Mourot, J; Duclos, M J; Lagarrigue, S; Gilbert, H; van Milgen, J

    2014-11-01

    The use and partition of feed energy are key elements in productive efficiency of pigs. This study aimed to determine whether dietary energy sources affect the partition of body lipids and tissue biochemical pathways of energy use between pigs differing in feed efficiency. Forty-eight barrows (pure Large White) from two divergent lines selected for residual feed intake (RFI), a measure of feed efficiency, were compared. From 74 d to 132 ± 0.5 d of age, pigs (n = 12 by line and by diet) were offered diets with equal protein and ME contents. A low fat, low fiber diet (LF) based on cereals and a high fat, high fiber diet (HF) where vegetal oils and wheat straw were used to partially substitute cereals, were compared. Irrespective of diet, gain to feed was 10% better (P percentage of PUFA, especially the EFA C18:2 and C18:3, was greater (P < 0.001) in backfat of HF-fed pigs. In both lines, these changes were associated with a marked decrease (P < 0.001) in the activities of two lipogenic enzymes, the fatty acid synthase (FASN) and the malic enzyme, in backfat. For the high RFI line, the hepatic lipid content was greater (P < 0.05) in pigs fed the HF diet than in pigs fed the LF diet, despite a reduced FASN activity (-32%; P < 0.001). In both lines, the HF diet also led to lower glycogen content (-70%) and lower glucokinase activity (-15%; P < 0.05) in the liver. These results show that dietary energy sources modified the partition of energy between liver, adipose tissue, and muscle in a way that was partly dependent of the genetics for feed efficiency, and changed the activity levels of biochemical pathways involved in lipid and glucose storage in tissues. PMID:25253805

  1. Perspectives on marine zooplankton lipids

    DEFF Research Database (Denmark)

    Kattner, G.; Hagen, W.; Lee, R.F.;

    2007-01-01

    climate change. The first addresses the role of lipids in membranes, storage lipids, and buoyancy with the following key question: How are the properties of membranes and deposits affected by the various types of lipids? The second deals with the importance of various types of lipids during reproduction...

  2. Hydrogenation Alternatives - Effects of Trans-Fatty-Acids and Stearic-Acid Versus Linoleic-Acid on Serum-Lipids and Lipoproteins in Humans

    NARCIS (Netherlands)

    Zock, P.L.; Katan, M.B.

    1992-01-01

    The objective of this study was to compare the effects of linoleic acid (cis,cis-C18:2(n-6)) and its hydrogenation products elaidic (trans-C18:1(n-9)) and stearic acid (C18:0) on serum lipoprotein levels in humans.Twenty-six men and 30 women, all nor

  3. Dietary folate and choline status differentially affect lipid metabolism and behavior-mediated neurotransmitters in young rats

    Science.gov (United States)

    The relationship between choline and folate metabolisms is an important issue due to the essential role of these nutrients in brain plasticity and cognitive functions. Present study was designed to investigate whether modification of the dietary folate-choline status in young rats would affect brain...

  4. LIPID PEROXIDATION IN PREECLAMPSIA

    OpenAIRE

    T. Sharmila Krishna; D. Raja Rajeswari; E. Venkat Rao; Sk. Deepthi; Naidu, J.N.

    2015-01-01

    Hypertension in pregnancy is a leading cause of both maternal and fetal mortality and morbidity. Preeclampsia is characterised by hypertension and proteinuria. Lipid peroxidation is an important factor in the pathophysiology of Preeclampsia. The present study was undertaken to determine Serum Malondialdehyde (MDA) levels , a product of lipid peroxidation , in clinically diagnosed Preeclamptic women(n=30) and the values were compared with that of Normotensive pregnant women (n=30) aged between...

  5. Comparison of the lipid properties of captive, healthy wild, and pansteatitis-affected wild Nile crocodiles (Crocodylus niloticus).

    Science.gov (United States)

    Osthoff, Gernot; Hugo, Arno; Bouwman, Henk; Buss, Peter; Govender, Danny; Joubert, Chris C; Swarts, Jannie C

    2010-01-01

    The results presented describe and compare the fatty acid composition and melting properties of captive, healthy wild, and pansteatitis-affected wild crocodiles (Crocodylus niloticus). Differences in fatty acid composition between intramuscular and adipose fat is noted in captive crocodiles, and the latter differs from wild crocodiles as a result of different diets. Adipose fat of healthy wild crocodiles differs minimally from diseased ones, respectively with 37.3+/-2.6% vs. 43.2+/-2.3% monounsaturated fatty acids, and 43.2+/-2.9% in dead crocodiles, while polyunsaturated fatty acids decrease from 27.3+/-1.9% to as low as 21.9+/-3.6% respectively. Of the unsaturated fatty acids 18:2n-6 decreased from 6.5+/-2.6% in unaffected crocodiles to 3.5+/-0.6% in highly affected and 3.2+/-0.4% in dead crocodiles, and 22:5n-3 from 2.8+/-0.6% to 1.8+/-0.3% and 2.2+/-0.3% respectively. The melting properties as determined by differential scanning calorimetry show that extracted adipose fat is a small degree softer in pansteatitis-affected tissue, specifically in the temperature range 7-36 degrees C, and does not contribute to the hard texture noted for adipose fat tissue of pansteatitis-affected animals. A high moisture content of 51.0+/-19.7% of the fat tissue of pansteatitis-affected animals vs.17.1+/-8.0% of healthy ones, suggests that physiological changes due to interstitial inflammation may contribute to the hard texture. PMID:19800020

  6. Effect of Protein-Lipid-Salt Interactions on Sodium Availability in the Mouth and Consequent Perception of Saltiness: As Affected by Hydration in Powders.

    Science.gov (United States)

    Yucel, Umut; Peterson, Devin G

    2015-09-01

    There is a broad need to reformulate lower sodium food products without affecting their original taste. The present study focuses on characterizing the role of protein-salt interactions on the salt release in low-moisture systems and saltiness perception during hydration. Sodium release from freeze-dried protein powders and emulsion powders formulated at different protein/lipid ratios (5:0 to 1:4) were characterized using a chromatography column modified with a porcine tongue. Emulsion systems with protein structured at the interface were found to have faster initial sodium release rates and faster hydration and were perceived to have a higher initial salt intensity with a lower salty aftertaste. In summary, exposure of the hydrophilic segments of the interface-structured proteins in emulsions was suggested to facilitate hydration and release of sodium during dissolution of low-moisture powder samples. PMID:26255668

  7. Effects of fish oil on serum lipid profile in dialysis patients: a systematic review and meta-analysis of randomized controlled trials

    OpenAIRE

    Zhu, Wei; Dong, Chongya; Du, Han; Zhang, He; Chen, Jie; Hu, Xiaohong; Hu, Feng

    2014-01-01

    Background The effects of fish oil supplements on lipid profile in dialysis patients are controversial. With increasing interest in the potential health benefits of fish oil, it is important to explore its real effects. Objective We aimed to identify and quantify the effects of fish oil on triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) in dialysis patients. Methods PubMed, EMBASE and the Cochrane Central...

  8. The Effect of Hydro-Alcoholic Extract of Grape Seed (Vitis vinifera) on Sugar and Lipids in Serum of Diabetic Rats

    OpenAIRE

    Zohre Ganjali; Fereshteh Javadian; Jasem Estakhr; Abbas Heidari

    2012-01-01

    Nowadays, no-drug treatments (medicinal plants) are novel therapeutic approaches in the treatment of diabetes. This study aimed at assessing the effect of Grape Seed extract on the blood glucose and lipid profile in diabetic rats. Thirty male adult rats were randomly selected and divided into three groups as nondiabetic control; diabetic control; diabetic rats treated with hydroalcoholic extract of grape seed. In diabetic groups, alloxan monohydrate (120 mg/kg) was injected intraperitoneally ...

  9. Effect of lean red meat from lamb v. lean white meat from chicken on the serum lipid profile: a randomised, cross-over study in women.

    Science.gov (United States)

    Mateo-Gallego, Rocio; Perez-Calahorra, Sofia; Cenarro, Ana; Bea, Ana M; Andres, Eva; Horno, Jaime; Ros, Emilio; Civeira, Fernando

    2012-05-01

    The main dietary guidelines recommend restricting total and saturated fat intake in the management of high blood cholesterol levels for cardiovascular risk. These recommendations are usually oversimplified by considering that all red meats should be limited and replaced by white meats. However, lean red meat can be as low in fat as white meat. We examined the effects of red meat (lean breed lamb) and lean white meat (chicken) intake on the lipid profile of a group of women with stable life conditions (nuns living in convents). An open-label, randomised, cross-over study was carried out in thirty-six nuns who consumed either lamb or chicken three times per week for 5-week periods with their usual diet. Clinical, dietary and biochemical variables were evaluated at baseline and the end of each diet period. A validated FFQ was used to assess nutrient intake and monitor compliance. The results showed neither between-diet differences in lipid responses nor differences from baseline in total cholesterol, LDL-cholesterol or TAG for any diet period. In conclusion, consumption of lean red meat (lamb) or lean white meat (chicken) as part of the usual diet is associated with a similar lipid response. These two foods can be exchanged in a healthy diet to increase palatability. PMID:21902857

  10. Selection for low erucic acid and genetic mapping of loci affecting the accumulation of very long-chain fatty acids in meadowfoam seed storage lipids.

    Science.gov (United States)

    Gandhi, S D; Kishore, V K; Crane, J M; Slabaugh, M B; Knapp, S J

    2009-06-01

    Erucic acid (22:1(13)) has been identified as an anti-nutritional compound in meadowfoam (Limnanthes alba) and other oilseeds in the Brassicales, a classification which has necessitated the development of low erucic acid cultivars for human consumption. The erucic acid concentrations of meadowfoam wild types (8%-24%) surpass industry standards for human consumption (affecting the accumulation of 22:1(13) and other very long-chain fatty acids (VLCFAs) in meadowfoam seed storage lipids. LE76, a low erucic acid line, was developed by 3 cycles of selection in an ethyl methanesulfonate-treated wildtype population. LE76 produced 3% 22:1(13), threefold less than the M0 population. Wildtype x LE76 F2 populations produced continuous, approximately normal erucic and dienoic acid distributions. Loss-of-function mutations apparently did not segregate and individuals with low 22:1(13) concentrations (affecting VLCFA profiles in seed storage lipids by genotyping and phenotyping wildtype x low erucic acid F2 progeny. Composite interval mapping identified 3 moderately large-effect erucic acid QTL. The low erucic acid parent transmitted favorable alleles for 2 of 3 QTL, suggesting low erucic acid cultivars can be developed by combining favorable alleles transmitted by wildtype and low erucic acid parents. PMID:19483773

  11. Interaction between dietary lipids and gut microbiota regulates hepatic cholesterol metabolism

    DEFF Research Database (Denmark)

    Caesar, Robert; Nygren, Heli; Orešič, Matej;

    2016-01-01

    The gut microbiota influences many aspects of host metabolism. We have previously shown that the presence of a gut microbiota remodels lipid composition. Here we investigated how interaction between gut microbiota and dietary lipids regulates lipid composition in the liver and plasma, and gene...... most lipid classes differed between mice fed lard and fish oil. However, the gut microbiota also affected lipid composition. The gut microbiota increased hepatic levels of cholesterol and cholesteryl esters in mice fed lard, but not in mice fed fish oil. Serum levels of cholesterol and cholesteryl...... esters were not affected by the gut microbiota. Genes encoding enzymes involved in cholesterol biosynthesis were downregulated by the gut microbiota in mice fed lard and were expressed at a low level in mice fed fish oil independent of microbial status. In summary, we show that gut microbiota...

  12. Factors affecting cerebral oxygenation in hemodialysis patients: cerebral oxygenation associates with pH, hemodialysis duration, serum albumin concentration, and diabetes mellitus.

    Directory of Open Access Journals (Sweden)

    Kiyonori Ito

    Full Text Available Patients undergoing hemodialysis (HD often develop cerebral disease complications. Furthermore, cerebral regional saturation of oxygen (rSO2 was previously reported to be significantly lower in HD patients than in healthy subjects. We aimed to identify the factors affecting the cerebral rSO2 in HD patients.Fifty-four HD patients (38 men and 16 women; mean age, 67.7 ± 1.2 years, HD duration, 6.5 ± 1.9 years were recruited. Cerebral rSO2 was monitored at the forehead before HD using an INVOS 5100C (Covidien Japan, Tokyo, Japan.The rSO2 levels were significantly lower in HD patients compared with healthy controls (49.5 ± 1.7% vs. 68.9 ± 1.6%, p <0.001. Multiple regression analysis showed that cerebral rSO2 independently associated with pH (standardized coefficient: -0.35, HD duration (standardized coefficient: -0.33, and serum albumin concentration (standardized coefficient: 0.28. Furthermore, the rSO2 was significantly lower in HD patients with diabetes mellitus (DM, compared with patients without DM (46.8 ± 1.7% vs. 52.1 ± 1.8%, p <0.05.In HD patients, cerebral rSO2 was affected by multiple factors, including pH, HD duration, and serum albumin concentration. Furthermore, this is the first report describing significantly lower levels of rSO2 in HD patients with DM than in those without DM.

  13. Evaluation of the Effects of Pinus koraiensis Needle Extracts on Serum Lipid and Oxidative Stress in Adults with Borderline Dyslipidemia: A Randomized, Double-Blind, and Placebo-Controlled Clinical Trial

    Science.gov (United States)

    Kim, Hyerang; Choue, Ryowon

    2016-01-01

    Background. Dyslipidemia has been well-known as a common metabolic disorder contributing to cardiovascular disease. The aim of this study was to evaluate the effect of the Pinus koraiensis needle extracts (PKE) on the blood cholesterol and oxidative stress. Method. We conducted a 12-week randomized, double-blinded controlled trial to examine the effect of PKE on blood lipid profiles in adults with borderline dyslipidemia. Thirty-three eligible persons were recruited and randomly assigned into PKE (n = 20) and placebo groups (n = 13). Serum lipids including total cholesterol, low-density lipoprotein- (LDL-) cholesterol, high-density lipoprotein- (HDL-) cholesterol, very low-density lipoprotein- (VLDL-) cholesterol, and triglyceride were measured before and after trial. Serum insulin, glucose, and antioxidant indicators were also analyzed before and after trial and anthropometry and blood pressure were measured every 4 weeks. Results. After 12 weeks, PKE statically significant decreases in systolic blood pressure (p < 0.05) and waist circumference (p < 0.05) were observed. Also, VLDL-cholesterol significantly decreased (from 24.4 ± 10.0 mg/dL at baseline to 18.4 ± 4.1 mg/dL after 12 weeks) (p < 0.05) and superoxide dismutase (SOD) increased (6.12 ± 0.41 U/mL to 9.06 ± 0.62 U/mL) (p < 0.01) in PKE group. However, after adjustment with WC, VLDL-cholesterol was not significant between groups (p = 0.095) and while SOD remained significant between groups (p = 0.013). Conclusion. The results show that PKE was effective in improving the superoxide dismutase in the individuals with borderline dyslipidemia.

  14. Prevalence study on glucose and serum lipids among obesity children in Haidian District of Beijing%北京海淀区肥胖儿童血糖血脂水平分析

    Institute of Scientific and Technical Information of China (English)

    蒲永兰; 邢彦; 孙冰洁; 黄娜; 沈艳辉

    2011-01-01

    目的 了解肥胖学生血糖、血脂水平,为心血管疾病的早期预防提供科学依据.方法 在生长发育监测数据基础上,根据BMI指数确定肥胖儿童,以学号建立抽样框架,随机抽取海淀区2 920名肥胖儿童进行调查.结果 调查对象血糖和血脂的平均水平处于参考值范围之内,男生血糖和三酰甘油水平高于女生,高密度脂蛋白水平低于女生(P值均<0.05);从指标异常情况来看,血糖、血脂水平均正常的比例不到50%,58.0%的调查对象具有1个及以上的指标异常,具有2种及以上异常指标的构成比例高达33.8%,总胆固醇的异常率最高(39.1%),高密度脂蛋白的异常率最低(4.2%).男生三酰甘油和高密度脂蛋白异常率均高于女生(P值均<0.05).BMI等级与肥胖儿章的血糖血脂指标异常数量之间存在一定的等级相关性(r=0.144,P<0.01).结论 肥胖儿童血糖、血脂指标异常率较高,异常指标的个数与BMI之间存在一定程度的相关性.%Objective To study the serum lipids levels of obesity children and to provide data for early prevention of cardiovascular heart disease in children. Methods A total of 3 000 obese children according BMI were sampled at random from the children's growth and development surveillance data in a district of Beijing, and fasting serum total cholesterol ( TC ), triglyceride ( TG ), high-density lipoprotein cholesterol ( HDL - C ),and low-density lipoprotein cholesterol ( LDL - C ) and serum glucose ( GLU ) were assayed. Results The average glucose and serum lipids of the obese children were at normal level, however, 58% of them were not all normal and 33.8% of them had at least two abnormal indicators. The abnormal rates of FPG, TC, TG, HDL - C, LDL - C were 11.0%, 39.1%, 17.7%, 4.2% and 17.8%, respectively. Level of FPG and TC, abnormal rates of TC and HDL - C were statistically significantly higher in male obesity children ( P < 0.05 ), and level of HDL

  15. Effect of Acacia nilotica Fruit Extract on Serum Glucose and Lipid Concentrations in Alloxan-induced Diabetic Rats

    OpenAIRE

    Abuelgassim O. Abuelgassim

    2013-01-01

    The present investigation was performed to study the effects of Acacia nilotica Delile (Fabaceae) fruit extract on serum concentrations of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglycerides and glucose in control and alloxan-induced diabetic rats. The rats were divided into four groups: Normal Control Rats (NC), normal control rats administered A. nilotica (NC+AN), Diabetic Control rats (DC) and diabetic rats administered A. nilot...

  16. Diet, nutrition intake, and metabolism in populations at high and low risk for colon cancer. Relationship of diet to serum lipids.

    Science.gov (United States)

    Kritchevsky, D; Tepper, S A; Goodman, G

    1984-10-01

    Serum cholesterol, high-density lipoprotein cholesterol, and triglycerides of three groups of Seventh-day Adventists (SDAs)--true vegetarians, lacto-ovo vegetarians, and nonvegetarians--and the general population were measured and related to age, sex, and diet. True vegetarian SDAs had the lowest cholesterol levels while cholesterol levels of the other three groups were similar. Triglyceride levels were highest in the true vegetarian SDAs and lowest in general population. Cholesterol levels rose with increasing age while percentage of high-density lipoprotein cholesterol fell. Female SDAs in all three dietary groups had higher cholesterol levels than males. In all four groups percentage of high-density lipoprotein cholesterol was higher in females than in males. In the male subjects, cholesterol levels varied linearly and inversely with carbohydrate intake. Protein and fat intake in true vegetarian SDAs and lacto-ovo vegetarian SDA groups were lower than in the nonvegetarian SDA and general population groups. The differences in protein and fat intake were reflected in elevations in serum cholesterol levels. In the female subjects, cholesterol levels were also inversely correlated with carbohydrate intake but not as distinctly as in the males. True vegetarian SDA females ingested less protein and fat than the other three groups and exhibited lower serum cholesterol levels. Cholesterol levels in lacto-ovo vegetarian SDA, nonvegetarian SDA, and general population female subjects were similar and did not reflect differences in protein or fat intake. The true vegetarian dietary lifestyle resulted in lowest cholesterol levels, however, if it was breeched to the least extent, cholesterol levels rose. Thus, intake of skim, low fat, or whole milk resulted in similar serum cholesterol levels as did low or high egg consumption. PMID:6486100

  17. Lipid profiles of blood serum and fatty acid composition of meat of hybrid duck fed diet supplemented with Noni (Morinda citrifolia fruit meal

    Directory of Open Access Journals (Sweden)

    David Kurniawan

    2015-09-01

    Full Text Available Noni fruit is a medicinal plant with biological activity like antioxidant that could potentially be used as a feed additive in poultry. This research investigated the effect of noni fruit powder as feed additive on lipid profiles of blood and meat fatty acid compositions of meat of hybrid duck. One hundred twenty 2-week-old hybrid ducks crossing between Peking and Khaki Campbell duck were subjected. They were randomly allotted to 24 experimental units. Each experimental unit was 70x80x40 cm in size and it was used for 5 ducks up to they reached 56 days of age. Each unit was equipped with waterer and feeder. The ducks were raised on litter-type floor. The basal experimental diet was formulated according to the standards of National Research Council (1994. The method used for this study was experimental with 4 different treatments in 6 replications. The treatments were as follow: P0: basal feed without supplementation of noni fruit powder as control; P1: basal feed + 1 % noni fruit powder; P2: basal feed + 2 % noni fruit powder; P3: basal feed + 3 % noni fruit powder. Data were analyzed by one-way of Completely Randomized Design ANOVA and if there was significant effect followed by Duncan’s Multiple Range Test. Result showed that using noni fruit powder as feed additive had no significant effect (P>0.05 on lipid profiles of blood and fatty acid composition of meat.

  18. MADD-FOLH1 Polymorphisms and Their Haplotypes with Serum Lipid Levels and the Risk of Coronary Heart Disease and Ischemic Stroke in a Chinese Han Population

    Directory of Open Access Journals (Sweden)

    Dong-Feng Wu

    2016-04-01

    Full Text Available This study aimed to detect the association of the MADD-FOLH1 single nucleotide polymorphisms (SNPs and their haplotypes with the risk of coronary heart disease (CHD and ischemic stroke (IS in a Chinese Han population. Six SNPs of rs7395662, rs326214, rs326217, rs1051006, rs3736101, and rs7120118 were genotyped in 584 CHD and 555 IS patients, and 596 healthy controls. The genotypic and allelic frequencies of the rs7395662 SNP were different between controls and patients, and the genotypes of the rs7395662 SNP were associated with the risk of CHD and IS in different genetic models. Six main haplotypes among the rs1051006, rs326214, rs326217, rs3736101, and rs7120118 SNPs were detected in our study population, the haplotypes of G-G-T-G-C and G-A-T-G-T were associated with an increased risk of CHD and IS, respectively. The subjects with rs7395662GG genotype in controls had higher triglyceride (TG and lower high-density lipoprotein cholesterol (HDL-C levels than the subjects with AA/AG genotypes. Several SNPs interacted with alcohol consumption to influence serum TG (rs326214, rs326217, and rs7120118 and HDL-C (rs7395662 levels. The SNP of rs3736101 interacted with cigarette smoking to modify serum HDL-C levels. The SNP of rs1051006 interacted with body mass index ≥24 kg/m2 to modulate serum low-density lipoprotein cholesterol levels. The interactions of several haplotypes and alcohol consumption on the risk of CHD and IS were also observed.

  19. Autophosphorylation of serine 608 in the p85 regulatory subunit of wild type or cancer-associated mutants of phosphoinositide 3-kinase does not affect its lipid kinase activity

    Directory of Open Access Journals (Sweden)

    Layton Meredith J

    2012-12-01

    Full Text Available Abstract Background The α-isoform of the Type 1A Phosphoinositide 3-kinases (PI3Kα has protein kinase activity as well as phosphoinositide lipid kinase activity. The best described substrate for its protein kinase activity is its regulatory subunit, p85α, which becomes phosphorylated on Serine 608. Phosphorylation of Serine 608 has been reported to down-regulate its lipid kinase activity. Results We have assessed whether oncogenic mutants of PI3Kα, which have up-regulated lipid kinase activity, have altered levels of Serine 608 phosphorylation compared to wild type PI3Kα, and whether differential phosphorylation of Serine 608 contributes to increased activity of oncogenic forms of PI3Kα with point mutations in the helical or the kinase domains. Despite markedly increased lipid kinase activity, protein kinase activity was not altered in oncogenic compared to wild type forms of PI3Kα. By manipulating levels of phosphorylation of Serine 608 in vitro, we found no evidence that the protein kinase activity of PI3Kα affects its phosphoinositide lipid kinase activity in either wild-type or oncogenic mutants of PI3Kα. Conclusions Phosphorylation of p85α S608 is not a significant regulator of wild-type or oncogenic PI3Kα lipid kinase activity.

  20. Lipid Profile

    Science.gov (United States)

    ... be limited. Home Visit Global Sites Search Help? Lipid Profile Share this page: Was this page helpful? Also ... as: Lipid Panel; Coronary Risk Panel Formal name: Lipid Profile Related tests: Cholesterol ; HDL Cholesterol ; LDL Cholesterol ; Triglycerides ; ...

  1. Association studies of several cholesterol-related genes (ABCA1, CETP and LIPC with serum lipids and risk of Alzheimer’s disease

    Directory of Open Access Journals (Sweden)

    Xiao Zhijie

    2012-11-01

    Full Text Available Abstract Objectives Accumulating evidence suggested that dysregulation of cholesterol homeostasis might be a major etiologic factor in initiating and promoting neurodegeneration in Alzheimer’s disease (AD. ATP-binding cassette transporter A1 (ABCA1, hepatic lipase (HL, coding genes named LIPC and cholesteryl ester transfer protein (CETP are important components of high-density lipoprotein (HDL metabolism and reverse cholesterol transport (RCT implicated in atherosclerosis and neurodegenerative diseases. In the present study, we will investigate the possible association of several common polymorphisms (ABCA1R219K, CETPTaqIB and LIPC-250 G/A with susceptibility to AD and plasma lipid levels. Methods Case–control study of 208 Han Chinese (104 AD patients and 104 non-demented controls from Changsha area in Hunan Province was performed using the PCR-RFLP analysis. Cognitive decline was assessed using Mini Mental State Examination (MMSE as a standardized method. Additionally, fasting lipid profile and the cognitive testing scores including Wechsler Memory Scale (WMS and Wisconsin Card Sorting Test (WCST were recorded. Results and conclusions We found significant differences among the genotype distributions of these three genes in AD patients when compared with controls. But after adjusting other factors, multivariate logistic regression analysis showed only ABCA1R219K (B = −0.903, P = 0.005, OR = 0.405, 95%CI:0.217-0.758 and LIPC-250 G/A variants(B = −0.905, P = 0.018, OR = 0.405, 95%CI:0.191-0.858 were associated with decreased AD risk. There were significantly higher levels of high-density lipoprotein cholesterol (HDL-C and apolipoproteinA-I in the carriers of KK genotype and K allele (P F = 5.598, P = 0.004, while -250 G/A polymorphisms had no significant effect on HDL-C. In total population, subjects carrying ABCA1219K allele or LIPC-250A allele obtained higher MMSE or WMS scores than non-carriers, however

  2. Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: a meta-analysis of 60 controlled trials

    NARCIS (Netherlands)

    Mensink, R.P.; Zock, P.L.; Kester, A.D.M.; Katan, M.B.

    2003-01-01

    Background: The effects of dietary fats on the risk of coronary artery disease (CAD) have traditionally been estimated from their effects on LDL cholesterol. Fats, however, also affect HDL cholesterol, and the ratio of total to HDL cholesterol is a more specific marker of CAD than is LDL cholesterol

  3. A diet rich in conjugated linoleic acid and butter increases lipid peroxidation but does not affect atherosclerotic, inflammatory, or diabetic risk markers in healthy young men

    DEFF Research Database (Denmark)

    Raff, Marianne; Tholstrup, Tine; Basu, Samar; Nonboe, Pernille; Sørensen, Martin Tang; Straarup, Ellen Marie

    2008-01-01

    fat intake. Our objective was to investigate the effect of CLA as part of a diet rich in butter as a source of milk fat on risk markers of atherosclerosis, inflammation, diabetes type 11, and lipid peroxidation. A total of 38 healthy young men were given a diet with 115g/d of CLA-rich fat (5.5 g/d CLA...... oil, a mixture of 39.4% cis9, trans11 and 38.5% trans10, cis12) or of control fat with a low content of CLA in a 5-wk double-blind, randomized, parallel intervention study. We collected blood and urine before and after the intervention. The fatty acid composition of plasma triacylglycerol, cholesterol......Intake of conjugated linoleic acid (CLA) has been demonstrated to beneficially affect risk markers of atherosclerosis and diabetes in rats. CLA is naturally found in milk fat, especially from cows fed a diet high in oleic acid, and increased CLA intake can occur concomitantly with increased milk...

  4. Effect of exogenous leptin on serum levels of lipids, glucose, renal and hepatic variables in both genders of obese and streptozotocin-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    Parichehr Hayatdavoudi

    2015-11-01

    Full Text Available Objective(s: Leptin exerts various effects on appetite and body weight. Disruption of the obesitygene is precedent to fatness. Insulin or glucose elevates leptin, but streptozotocin reduces it. However, controversial data exist for the effects of leptin on diabetes and leptin level in each gender. Leptin can damage the kidney function but little evidence exists for its hepatic effects. The aim of this study was to investigate the probable sex-dependent differences in blood sugar levels, lipid profile, and renal and hepatic biochemical factors in the obesity and streptozotocin-induced diabetic rats after leptin administration. Materials and Methods: Wistar rats of both sexes were randomly divided into two groups, namely obese and diabetic rats. Each group was further divided into male and female subgroups. Extra fat and carbohydrate was added to the diet to induce obesity. Furthermore, streptozotocin (55 mg/kg, IP was injected to induce diabetes. The treatment groups received leptin (0.1 mg/kg SC for 10 days, and then, blood samples were taken from the orbital sinus for laboratory evaluations. Results: Leptin resulted in a significant weight loss in both sexes (P

  5. Does the time interval between antimüllerian hormone serum sampling and initiation of ovarian stimulation affect its predictive ability in in vitro fertilization-intracytoplasmic sperm injection cycles with a gonadotropin-releasing hormone antagonist?

    DEFF Research Database (Denmark)

    Polyzos, Nikolaos P; Nelson, Scott M; Stoop, Dominic;

    2013-01-01

    To investigate whether the time interval between serum antimüllerian hormone (AMH) sampling and initiation of ovarian stimulation for in vitro fertilization-intracytoplasmic sperm injection (IVF-ICSI) may affect the predictive ability of the marker for low and excessive ovarian response....

  6. 冬泳对老年人身体成分和血脂代谢的影响%Effect of Swimming on Body Composition and Serum Lipid Metabolism in the Elderly

    Institute of Scientific and Technical Information of China (English)

    景会锋; 王小梅

    2015-01-01

    Study on the effect of swimming on body composition and metabolism of serum lipids in the elderly. Select who frequently took part in winter swimming in 50 people aged 60~69 years for swimming in groups of older per-sons,and selected peer group system participating in physical exercise in healthy elderly 50-free control group, body composition and blood test. And control group compared to,swimming group of elderly,thin weight,and mus-cle volume,and body total water,and protein quality,and waist hip than( WHR),and high-density fat protein ( HDL-C)content were obviously increased,mineralsare obviously increased and fat percentage,and obesity rate, and total cholesterol( TC),and glycerol three ester( TG)and low density fat protein( LDL-C)content were obvi-ously reduced;and age,and height,and weight,and body quality index( BMI),and ratio of extra-cellular fluid and other indicators had no significant difference. The system of winter swimming exercise improves body composi-tion and serum lipid metabolism in the elderly.%探讨冬泳对老年人身体成分和血脂代谢的影响。选取经常参加冬泳的50名年龄在60~69岁的老年人为冬泳组,同时选取同年龄组无系统参加体育锻炼的健康老年人50名为对照组,测试身体成分和血脂。结果与对照组相比,冬泳组的老年人的骨骼肌肉量、蛋白质量、腰臀比( WHR)、高密度脂蛋白( HDL-C)和矿物质含量均明显升高;而脂肪量、肥胖率、总胆固醇( TC)、甘油三酯( TG)和低密度脂蛋白( LDL-C)含量均明显降低;而年龄、身高、体重、身体总水分、身体质量指数( BMI)等指标无明显差异。系统的冬泳锻炼能改善老年人的身体成分和血脂代谢。

  7. Effects of polychlorinated biphenyls and lipemia on serum analytes

    Energy Technology Data Exchange (ETDEWEB)

    Steinberg, K.K.; Freni-Titulaer, L.W.J.; Rogers, T.N.; Burse, V.W.; Mueller, P.W.; Stehr, P.A.; Miller, D.T.; Steele, G.

    1986-01-01

    Twelve serum analytes (triglycerides, cholesterol, total and conjugated bilirubin, high-density-lipoprotein cholesterol (HDL-C) alkaline phosphatase (AP), gammaglutamyl transferase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), ..beta..-glucoronidase (..beta..-glu), alanine aminopeptidase (AAP), and 5'-nucleotidase (5'nuc)) were measured to investigate their correlation with exposure to polychlorinated biphenyls (PCBs) and 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT). The relationship between serum lipids, lipophilic toxicants, and the analytes was also evaluated. The ..beta..-glue, 5'nuc, triglycerides, cholesterol, and total bilirubin correlated positively and significantly with log concentrations of serum total PCBs and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE), a metabolite of DDT. The more highly chlorinated PCBs had significant, positive correlations with several serum analytes, but the less chlorinated PCBs correlated significantly and negatively only with HDL-cholesterol. Triglyercide- and cholesterol-rich lipoproteins were added to serum to determine the effects of lipids on these assays. Several were spuriously elevated. AP and ..beta..-glu were not affected by lipoprotein addition with the methods used in this study. AAP was increased significantly only at triglyceide concentrations exceeding 400 mg/dl.

  8. Effect of serum, cholesterol and low density lipoprotein on the functionality and structure of lung surfactant films.

    Science.gov (United States)

    Nahak, Prasant; Nag, Kaushik; Hillier, Ashley; Devraj, Ravi; Thompson, David W; Manna, Kausik; Makino, Kimiko; Ohshima, Hiroyuki; Nakahara, Hiromichi; Shibata, Osamu; Panda, Amiya Kumar

    2014-01-01

    Lung surfactant is a complex mixture of lipid and protein, responsible for alveolar stability, becomes dysfunctional due to alteration of its structure and function by leaked serum materials in disease. Serum proteins, cholesterol and low density lipoprotein (LDL) were studied with bovine lipid extract surfactant (BLES) using Langmuir films, and bilayer dispersions using Raman spectroscopy. While small amount of cholesterol (10 wt%) and LDL did not significantly affect the adsorption and surface tension lowering properties of BLES. However serum lipids, whole serum as well as higher amounts of cholesterol, and LDL dramatically altered the surface properties of BLES films, as well as gel-fluid structures formed in such films observed using atomic force microscopy (AFM). Raman-spectroscopic studies revealed that serum proteins, LDL and excess cholesterol had fluidizing effects on BLES bilayers dispersion, monitored from the changes in hydrocarbon vibrational modes during gel-fluid thermal phase transitions. This study clearly suggests that patho-physiological amounts of serum lipids (and not proteins) significantly alter the molecular arrangement of surfactant in films and bilayers, and can be used to model lung disease. PMID:25409691

  9. Bu-Shen-Ning-Xin Decoction ameliorated the osteoporotic phenotype of ovariectomized mice without affecting the serum estrogen concentration or uterus

    Directory of Open Access Journals (Sweden)

    Wang L

    2015-08-01

    Full Text Available Ling Wang,1,2,* Xue-Min Qiu,1,2,* Yu-Yan Gui,1,2 Ying-Ping Xu,1,2 Hans-Jürgen Gober,3 Da-Jin Li1 1Laboratory for Reproductive Immunology, Hospital and Institute of Obstetrics and Gynecology, IBS, Fudan University Shanghai Medical College, 2Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, People’s Republic of China; 3Department of Pharmacy, Wagner Jauregg Hospital and Children’s Hospital, Linz, Austria *These authors contributed equally to this work Introduction: Bu-Shen-Ning-Xin Decoction (BSNXD, a traditional Chinese medicinal composition, has been used as a remedy for postmenopausal osteoporosis, but its effects on bone metabolism and the uterus have not been reported.Purpose: We aimed to determine the respective effects of BSNXD on the bones and the uterus of ovariectomized (OVX mice to evaluate the efficacy and safety of this herbal formula.Materials and methods: Postmenopausal osteoporosis animal models that were generated by ovariectomy were treated with BSNXD. Dual-energy X-ray absorptiometry was performed to analyze the bone mineral density, and histomorphometric analysis was performed to measure the parameters related to bone metabolism. Calcein labeling was performed to detect bone formation. The uteruses from the mice were weighed, and the histomorphometry was analyzed. Drug-derived serum was prepared to assess the 17-β-estradiol concentration via enzyme immunoassay.Results: BSNXD administration ameliorated the osteoporotic phenotype of OVX mice, as evidenced by an increase in the bone mineral density and bone volume; these effects could not be abolished by the administration of the aromatase inhibitor letrozole. Moreover, BSNXD had no effect on the serum estrogen concentration or uterus.Conclusion: These results suggest that BSNXD has ameliorating effects on bone loss due to estrogen deprivation without affecting the peripheral blood estrogen concentration or the uterus in OVX mice. Keywords

  10. Sunlight–derived vitamin D affects interleukin-4 level, T helper 2 serum cytokines, in patients with Graves’ disease: a prospective cohort study

    Directory of Open Access Journals (Sweden)

    Dyah Purnamasari

    2015-12-01

    Full Text Available Background: Graves’ disease (GD is the most common autoimmune disease leading to hyperthyroidism. The role of Th1/Th2 pathways balance in GD is still controversial. Vitamin D is reported to have an effect on those pathways. This study aims to examine the effect of sunlight exposure on vitamin D 25(OH level and Th1 and Th2 pathway-derived cytokines in GD patients. Methods: A prospective cohort study was conducted on 32 GD patients to compare the effect of sunlight exposure on vitamin D level and cytokines of Th1 and Th2 pathways between exposed and unexposed groups. Exposed group received sunlight exposure three times a week for 30 minutes each between 9–11 a.m for 1 month. Thyroid stimulating hormone (TSH, free thyroxin (fT4, vitamin D 25(OH, interferon-γ (IFN-γ and interleukin-4 (IL-4 serum levels, were investigated before and after one month of sunlight exposure. Paired t-test or Mann Whitney test were used to analyze the difference between exposed and unexposed GD groups before and after sun exposure.Results: One month of sunlight exposure increased vitamin D 25(OH level by 27.90% among exposed GD group (15.34 ng/mL to 19.62 ng/mL, p<0.001. Meanwhile, unexposed GD group’s vitamin D 25(OH level decreased from 20.48 ng/mL to 18.86 ng/mL (p<0.001. Increased vitamin D 25(OH level in exposed group was not accompanied by the increase of IL-4 level after sunlight exposure.Conclusion: Sunlight exposure increases vitamin D 25(OH serum level and may affect the level of IL-4, Th2 pathway-derived cytokine, in patients with GD. However, the role of sunlight-derived vitamin D on IFN-γ in GD patients can not be concluded in this study.

  11. Novel replication-competent circular DNA molecules from healthy cattle serum and milk and multiple sclerosis-affected human brain tissue.

    Science.gov (United States)

    Whitley, Corinna; Gunst, Karin; Müller, Hermann; Funk, Mathis; Zur Hausen, Harald; de Villiers, Ethel-Michele

    2014-01-01

    Epidemiological data point to the involvement of a cow milk factor in the etiology of multiple sclerosis (MS). Eleven circular DNA molecules closely related to transmissible spongiform encephalopathy (TSE)-associated isolate Sphinx 1.76 were isolated from healthy cattle serum, cow milk, and serum and brain tissue from MS patients. PMID:25169859

  12. Comparison of serum creatine kinase estimation with short tandem repeats based linkage analysis in carriers and affected children of duchenne muscular dystrophy

    International Nuclear Information System (INIS)

    Background: Duchenne Muscular Dystrophy (DMD) is an X-linked recessive lethal, genetic disorder characterised by progressive weakness of skeletal muscles which is untreatable and transmitted to males by carrier females. Advances in laboratory techniques now focus direct mutational analysis as the most reliable and indirect analysis based on Short Tandem Repeats (STR) based linkage analysis as feasible, inexpensive, and efficient method for carrier detection and prenatal diagnosis. The objective of this study was to compare the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and diagnostic efficiency of Serum Creatine Kinase (SCK) with Short Tandem Repeats (STR based linkage analysis in carriers and affected children of Duchenne Muscular Dystrophy. Methods: The study was carried out from Dec 2006 to Dec 2007 in families having index clinical cases of DMD who were referred from different hospitals for evaluation/workup of DMD. SCK was done as a preliminary investigation in all index cases. The PCR assay with STR based linkage analysis with Intron 44, 45, 49 and 50 of DMD gene were performed in all families. Six families were informative with Intron 44 of DMD gene and one family was non-informative with all four intronic markers of DMD. SCK analyses were done in all the family members and compared with PCR analysis in informative families. SCK was not performed on Chorionic villous sample (CVS) done for prenatal diagnosis of DMD, and CVS and non-informative family members were excluded from the study. Results: In carriers of DMD, the sensitivity and negative predictive value of SCK were 33.3%, and specificity and positive predictive were 100% with diagnostic efficiency of 50%. In affected cases of DMD the sensitivity and negative predictive value of SCK were 100%, and specificity and positive predictive were 91% and 88.8% respectively and diagnostic efficiency of 94.1%. Conclusion: The SCK is an excellent screening test for

  13. Factors Affecting Gender Differences in the Association between Health-Related Quality of Life and Metabolic Syndrome Components: Tehran Lipid and Glucose Study.

    Directory of Open Access Journals (Sweden)

    Parisa Amiri

    Full Text Available Using structural equation modeling, this study is one of the first efforts aimed at assessing influential factors causing gender differences in the association between health-related quality of life (HRQoL and metabolic syndrome.A sample of 950 adults, from Tehran Lipid and Glucose Study were recruited for this cross sectional study in 2005-2007. Health-related quality of life was assessed using the Iranian version of SF-36. Metabolic syndrome components (MetSCs and physical and mental HRQoL were considered as continuous latent constructs explaining the variances of their observed components. Structural equation modeling was performed to examine the association between the constructs of MetSCs and the physical and mental HRQoL within the two gender groups.Based on the primary hypothesis, MetSCs and HRQoL were fitted in a model. The negative effect of MetSCs on HRQoL was found to be significant only in the physical domain and only in women. The proportion of all the cardio-metabolic risk factors as well as subscales of physical HRQoL that have been explained via the two constructs of MetSCs and HRQoL, respectively, were significantly higher in women. Physical activity in both men (β = 3.19, p<0.05 and women (β = 3.94, p<0.05, age (β = -3.28, p<0.05, education (β = 2.63, p<0.05 only in women and smoking (β = 2.28, p<0.05 just in men, directly affected physical HRQoL. Regarding the mental domain, physical activity (β = 3.37, p<0.05 and marital status (β = 3.44, p<0.05 in women and age (β = 2.01, p<0.05 in men were direct effective factors. Age and education in women as well as smoking in men indirectly affected physical HRQoL via MetSCs.Gender differences in the association between MetSCs and physical HRQoL could mostly be attributed to the different structures of both MetSCs and physical HRQoL constructs in men and women. Age and smoking are the most important socio-behavioral factors which could affect this gender-specific association in

  14. The relationship between serum TSH(thyroid stimulating hormone) and lipid metabolism in euthyroid subjects%正常人群甲状腺功能与脂肪代谢的相关性分析

    Institute of Scientific and Technical Information of China (English)

    张皎月; 陈璐璐; 孙晖; 郑涓; 胡祥; 王素星; 陈婷

    2013-01-01

    目的 研究正常人群中血清促甲状腺激素(TSH)与脂肪代谢的关系. 方法 对武汉市1322名正常人群进行体格检查、血清TSH测定及肝脏超声检查. 结果 超重(A)组血清TSH水平高于对照(B)组.女性TSH>2.5 mI U/L(B)组体重、BMI、WC、体脂含量高于TSH≤2.5mI U/L(A)组.体脂含量、BMI是TSH的独立影响因素.TSH不是影响非酒精性脂肪肝患病的独立危险因素. 结论 女性正常范围内的血清TSH与肥胖密切相关;血清TSH与非酒精性脂肪肝的患病风险无关.%Objective To explore the relationship between serum TSH level and lipid metabolism in euthyroid subjects. Methods The 1322 subjects were enrolled in the trial. Physical, laboratory, and liver ultrasonography examinations were carried out for all of them. Results Serum TSH level was significantly higher in overweight (A)group than in control (B)group. Levels of body weight, BMI, waist circumference, and percentage of body fat were increased in TSH > 2. 5 ml U/L women (B) group compared with those in TSH<2. 5 ml U/L women (A)group. Percentage of body fat and BMI contributed significantly to the variance of TSH. TSH level was not the independent risk factor of non-alcoholic fatty liver disease. Conclusion Serum TSH in normal range is significantly correlated with obesity in women. And the change of TSH will not increase the prevalence of non-alcoholic fatty liver disease.

  15. 内源性n-3多不饱和脂肪酸对fat-1转基因小鼠血糖血脂的影响%Effects of Endogenous n-3 Polyunsaturated Fatty Acids (PUFAs) on Blood Glucose and Serum Lipid in fat-1 Transgenic Mouse Model

    Institute of Scientific and Technical Information of China (English)

    周飞; 张晓宏; 邹祖全; 张才乔

    2015-01-01

    利用fat-1转基因小鼠模型,研究内源性n-3多不饱和脂肪酸(n-3 PUFAs)的血糖血脂调节作用.采用fat-1转基因小鼠和C57BL/6野生型小鼠喂食高n-6、低n-3 PUFAs的标准配方饲料4周,然后2组小鼠给予高糖饮液自由饮用4周,每周测体重.第8周末,取血离心测血糖、胰岛素、甘油三脂(TG)、胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)水平.结果表明:fat-1转基因小鼠体重增加幅度、空腹血糖值、血清胰岛素、胰岛素抵抗指数、血清TC、TG、HDL-C、LDL-C水平明显低于野生型小鼠(P<0.05).认为n-3 PUFAs能抑制体重增长,降低小鼠血糖、血脂和胰岛素抵抗,起到调节血糖、血脂的作用.%In this study, we investigate the effects of endogenous n-3 PUFAs on blood glucose and serum lipid in fat-1 transgenic mouse model. The transgenic and wild type mice are maintained on a 10%corn oil diet which has high ratio of n-6/n-3 polyunsaturated fatty acids for 4 weeks. Then make them drink high sugar pinocytosis freely for another four weeks. On the eighth weekend, serum glucose, insulin, serum triglyceride (TG), serum total cholesterol (TC), serum high-density lipoprotein cholesterol (HDL-C), serum low-density lipoprotein cholesterol (LDL-C) levels of blood serum are tested after blood collection and centrifugation. The weight gain rate, fasting blood sugar and serum insulin levels, insulin resistance index, serum TC, TG, HDL-C, LDL-C of fat-1 transgenic mice are found to be significantly lower than those of wild type mice (P<0.05). The tests indicate that n-3 polyunsaturated fatty acids can reduce the blood glucose and serum lipid, which may play a vital role in regulating blood lipid and decreasing insulin resistance.

  16. 高龄产妇妊娠晚期血脂水平与围产结局%Serum lipid levels in Advanced maternal during late pregnancy and perinatal outcome

    Institute of Scientific and Technical Information of China (English)

    丁宁; 王超; 李琳

    2014-01-01

    目的:探讨高龄产妇妊娠血脂水平与围产结局。方法:将在本院住院治疗的113例妊娠晚期高龄孕产妇(研究组)作为观察对象,与同期妊娠的孕产妇(对照组)比较,对两组妊娠期孕产妇的晚期血脂水平与围产结局进行观察。结果:妊娠晚期高龄产妇较正常组血清血脂水平在总胆固醇(TC),低密度脂蛋白(LDL-C),高密度脂蛋白(HDL-C),统计学上差异无意义(P>0.05);而在甘油三酯(TG)方面差异明显(P0.05);while it had significant difference in the triglyceride (TG) (P<0.05). It also had significant differences in the perinatal outcome of fetal distress, cesarean section and pregnancy induced hypertension, diabetes and other complications (P<0.05). Conclusion:The effective control of serum lipid levels can improve the perinatal outcome of women with advanced maternal age.

  17. Additions of caffeic acid, ascorbyl palmitate or gamma-tocopherol to fish oil-enriched energy bars affect lipid oxidation differently

    DEFF Research Database (Denmark)

    Horn, Anna Frisenfeldt; Nielsen, Nina Skall; Jacobsen, Charlotte

    2009-01-01

    The objectives of the study were to investigate the effects of caffeic acid, ascorbyl palmitate and gamma-tocopherol on protection of fish oil-enriched energy bars against lipid oxidation during storage for 10 weeks at room temperature. The lipophilic gamma-tocopherol reduced lipid oxidation during......, or the hydrophilic caffeic acid, or the amphiphilic ascorbyl palmitate at concentrations of 75, 150 and 300 mu g/g fish oil. Prooxidative effects were observed as an increase in the formation of lipid hydroperoxides and volatile secondary oxidation products, as well as the development of rancid off...

  18. A postnatal diet containing phospholipids, processed to yield large, phospholipid-coated lipid droplets, affects specific cognitive behaviors in healthy male mice

    NARCIS (Netherlands)

    Schipper, Lidewij; van Dijk, Gertjan; Broersen, Laus M; Loos, Maarten; Bartke, Nana; Scheurink, Anton J.W.; van der Beek, Eline M

    2016-01-01

    BACKGROUND: Infant cognitive development can be positively influenced by breastfeeding rather than formula feeding. The composition of breast milk, especially lipid quality, and the duration of breastfeeding have been linked to this effect. OBJECTIVE: We investigated whether the physical properties

  19. Oxidative stability, lipid composition and nutritional value of ruminant meat as affected by animal feeding system, sex and common household treatments

    OpenAIRE

    Boe, Roberta

    2012-01-01

    Ruminant meats have been suffered from a negative health image related to the nature of their lipid fraction, mainly due to the higher content in SFA. Even so, ruminant edible fats are the major natural source of CLA, provide proteins of high biological value and important micronutrients, such as A, group B, D and E vitamins, minerals and essential fatty acids all these components are associated with potential health benefits. Oxidation of lipids in foods is the major biochemical process that...

  20. Evaluation of factors affecting on lipid extraction for recovery of fatty acids from Nannochloropsis oculata micro-algae to biodiesel production

    Directory of Open Access Journals (Sweden)

    Mohammad Malakootian

    2014-11-01

    Full Text Available Background: This study aimed at determining the appropriate method for dewatering and drying biomass and selecting a suitable organic solvent for lipid extraction. Methods: NannochloropsisOculata was cultured in Gillard F/2 medium and after reaching the end of the stationary growth phase, algal biomass was separated from aqueous by centrifuge and dried through three methods: Oven, Air-dried and Lyophilized. Soxhlet apparatus achieved lipid extraction of all samples: diethyl ether, n-hexane and n-pentane using three solvents. At each stage, the quantity and quality of the extracted lipids were determined by Gas Chromatography. Results: In all three drying methods, palmitic acid and palmitoleic acid, and most significantly fatty acid composition of microalgae were extracted. The fatty acid composition of palmitic acid extracted by Diethyl ether was significantly more than the other two solvents. Maximum production of triglyceride was observed in Lyophilized and air-dried microalgae where lipid extraction was performed with diethyl ether solvents and are 75.03% and 76.72% of fatty acid. Conclusion: The use of Lyophilized method for dewatering and drying of biomass and Diethyl ether as solvent for the extraction of lipids from biomass, studied in this paper, as compared to other methods, had higher yields and researches proved that the production of biodiesel from microalgae’s lipid was more efficient.

  1. Body measurements and serum lipid profile of overweight adult dogs fed diet with containing conjugated linoleic acid Medidas corporais e perfil lipídico plasmático de cães adultos com sobrepeso alimentados com dieta contendo ácido linoléico conjugado

    OpenAIRE

    Nancy Lorena Montaño Rivera; Ananda Portela Félix; Fabiano Montiani Ferreira; Ana Vitória Fisher da Silva; Alex Maiorka

    2011-01-01

    Studies with human beings and animals have shown that dietary conjugated linoleic acid (CLA) promotes changes in body structure, markedly, body fat reduction. This research aimed to assess the effect of CLA (60% of conjugated linoleic acid methyl ester, at the proportion of 1:1 of the 9, 11: 10, 12 isomers) on changes in body weight, subcutaneous tissue thickness, body fat mass and concentration of serum lipids (triacylglycerols, total cholesterol, LDL and HDL) in overweight dogs. Dogs of dif...

  2. Identification of Plants That Inhibit Lipid Droplet Formation in Liver Cells: Rubus suavissimus Leaf Extract Protects Mice from High-Fat Diet-Induced Fatty Liver by Directly Affecting Liver Cells

    Science.gov (United States)

    Takahashi, Tomohiro; Sugawara, Wataru; Takiguchi, Yuya; Takizawa, Kento; Nakabayashi, Ami; Nakamura, Mitsuo; Nagano-Ito, Michiyo; Ichikawa, Shinichi

    2016-01-01

    Fatty liver disease is a condition in which abnormally large numbers of lipid droplets accumulate in liver cells. Fatty liver disease induces inflammation under conditions of oxidative stress and may result in cancer. To identify plants that protect against fatty liver disease, we examined the inhibitory effects of plant extracts on lipid droplet formation in mouse hepatoma cells. A screen of 98 water extracts of plants revealed 4 extracts with inhibitory effects. One of these extracts, Rubus suavissimus S. Lee (Tien-cha or Chinese sweet tea) leaf extract, which showed strong inhibitory effects, was tested in a mouse fatty liver model. In these mouse experiments, intake of the plant extract significantly protected mice against fatty liver disease without affecting body weight gain. Our results suggest that RSE directly affects liver cells and protects them from fatty liver disease.

  3. Epidemiological investigation of the serum lipid level and its abnormality in the residents in Malu Community%马陆镇居民血脂水平和异常流行病学调查

    Institute of Scientific and Technical Information of China (English)

    翁立立; 陆萍

    2014-01-01

    目的:调查嘉定区马陆镇30岁以上居民血脂水平和异常现状,为社区心脑血管疾病防治提供资料。方法:对辖区7014名健康体检者检测血脂水平,分析血脂异常与性别、年龄段关系。结果:TC、TG、HDL-C及LDL-C水平分别为(5.08±1.01)mmol/L、(1.51±1.19)mmol/L、(1.46±0.38)mmol/L、(3.07±0.85) mmol/L;异常率分别为41.2%、13.5%、10.1%、31.7%。结论:马陆镇30岁以上居民血脂异常率高,以高TC、LDL-C为主,应做好早期综合防治。%Objective:To investigate the lipid level and its abnormality in the residents over the age of 30 in Malu Town, Jiading District to provide the information for the community to prevent and treat the cardiovascular diseases. Methods:The serum lipid was detected, and its abnormality, gender and age were analyzed. Results:The levels of TC, TG, HDL-C and LDL-C were (5.08±1.01) mmol/L, (1.51±1.19) mmol/L, (1.46±0.38) mmol/L, and (3.07±0.85) mmol/L, whose abnormal rates were 41.2%, 13.5%, 10.1%and 31.7%, respectively. Conclusion:The rates of dyslipidemia of the residents over the age of 30 in Malu Town are high and TC, and LDL-C are higher among them mainly. The early comprehensive prevention and treatment should be prepared.

  4. Short-term overt hypothyroidism affect on lipids after thyroxine-withdrawal in patients with differentiated thyroid carcinoma%骨巨细胞瘤软组织内复发两例报道及文献复习

    Institute of Scientific and Technical Information of China (English)

    Hongyu Wu; Shuyao Zuo; Chao Ma; Bin Liu; Fengyu Wu; Simin Liu; Qin Zhang; Xifu Lan

    2009-01-01

    Objective: The aim of the study was to investigate the effects of short-term overt hypothyroidism on lipids after thyroxine-withdrawal in patients with iatrogenically induced hypothyroidism before radioiodine treatment for differentiated thyroid carcinoma (DTC). Methods: Thirty patients with a history of differentiated thyroid carcinoma on thyroid-stimulating hormone (TSH)-suppressive thyroxine replacement therapy were studied. Blood sample were taken before and 4 weeks after withdrawal of thyroxine substitution. Venous blood was drawn after an overnight fast and analyzed for serum free T4 (FT4), free T3 (FT3), thyroid-stimulating hormone (TSH), total cholesterol (TC), triglyceddes (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and apolipoprotein A1 (ApoA1), apolipoprotein B (ApoB). Fifty healthy people matched for age and gender were controls. Their blood samples were taken only once. Results: After thyroxine-withdrawal, the patients presented with increased serum TSH and low serum FT4 and FT3 levels compared with controls. Serum TG, TC, LDL, HDL, APOB and Lp(a) increased after thyroxine withdrawal, reaching statistical significant dif-ferences with previous evaluation. However, when compared to euthyroid controls, only TC, LDL and ApoB were increased when patients were hypothyroidism. No changes were observed in ApoA1 in patients dudng thyroxine withdrawal, or when comparing the values observed in patients to those of euthyroid controls. Conclusion: TG, TC, LDL, HDL, APO8 and Lp(a) were increased during short-term overt hypothyroidism.

  5. Efeito da dieta e do ciclismo indoor sobre a composição corporal e nível sérico lipídico Effect of diet and indoor cycling on body composition and serum lipid

    Directory of Open Access Journals (Sweden)

    Valéria Sales do Valle

    2010-08-01

    ,200 kcal. The study lasted 12 weeks. We used descriptive statistics (mean and standard deviation and inferential statistics (Student's t test. The level of significance was p < 0.05. RESULTS: The groups CI, CD and D significantly reduced the mean anthropometric variables after 12 weeks of intervention (body mass, fat percentage and body mass index, and serum levels of total cholesterol and triglycerides. HDL cholesterol increased significantly for groups CI and CD. CONCLUSION: According to the results, the indoor cycling and the low-calorie diet helped fight overweight and control serum lipids.

  6. Pinto Bean Consumption Changes SCFA Profiles in Fecal Fermentations, Bacterial Populations of the Lower Bowel, and Lipid Profiles in Blood of Humans

    Science.gov (United States)

    Background: Beans improve serum lipids and may reduce the risk of colon cancer by increasing colonic short chain fatty acid (SCFAs) formation. Objective: We assessed whether pinto bean consumption affects 1) in vitro fecal bacterial fermentation and production of SCFAs, 2) colonic bacterial populat...

  7. 成人身体成分与血脂、血糖关系的探讨%Analysis of the relationship between body composition and fasting serum lipids, fasting blood glucose in adult

    Institute of Scientific and Technical Information of China (English)

    李秋桂; 姬芳勤

    2014-01-01

    目的:分析成人身体成分与血脂、血糖指标之间的关系方法选择成年体检者490名,排除严重心脑肝肾疾病,肿瘤患者,急性炎症患者及长期服药者等。通过Biospace InBody 220人体成分分析仪对身体水分、蛋白质、无机盐、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比等指标进行测定。同时测定空腹血糖、血脂指标。所有指标的测定均由我院医护人员经过统一培训后进行操作。结果男女性别年龄无差异(p>0.05)。除体脂肪外,男女性别间身体水分、蛋白质、无机盐、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比均有显著差异(均p<0.001)。蛋白质、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比与空腹血糖呈正相关(均p<0.05)。身体水分、蛋白质、无机盐、体脂肪、体重、骨骼肌肉量、体质指数、体脂百分比、腰臀脂肪比均可对血脂(总胆固醇、甘油三酯)产生影响(均p<0.05)。剔除性别因素后,对身体成分与血糖、血脂各指标进行偏相关分析,体脂肪、体重、体质指数、体脂百分比、腰臀脂肪比仍然与血糖、总胆固醇、甘油三酯呈正相关(均p<0.05)。结论成人身体成分与血糖、血脂指标密切相关。通过身体成分的分析,可尽早预防代谢相关性疾病。%Objective To analyze the relationship between body composition and fasting serum lipids, fasting blood glucose in adult. Methods Select 490 adult, eliminate the subjects who has the serious cardiovascular diseases, liver diseases , kidney diseases, cancer and the subjects with acute inflammation and long term medication etc. Body moisture, protein, inorganic salt, fat, skeletal muscle weight, body mass index, percentage of body fat, waist and hip fat ratio was determined by Biospace InBody 220. Measure the

  8. The extraction and serum lipids reducing function of fucoidan from seaweed Costaria costata%多肋藻岩藻聚糖硫酸酯的提取及其降血脂作用研究

    Institute of Scientific and Technical Information of China (English)

    李鸶鸶; 汪秋宽; 何云海; 任丹丹; 张泽宇

    2013-01-01

    of the mice serum triglyceride (TG) and low density lipoprotein (LDL-C) at a dose of over 500 mg/ ( kg · d ) (P< 0.05). The fucoidan extract from the Costaria costata had significant inhibition of the three indices of hyperlipi-demia mentioned obove, indicating that the fucoidan functions as high-lipid reducing.

  9. Diacylglycerol oil does not affect portal vein transport of nonesterified fatty acids but decreases the postprandial plasma lipid response in catheterized Pigs

    DEFF Research Database (Denmark)

    Kristensen, Janni Brogaard; Jørgensen, Henry; Mu, Huiling

    2006-01-01

    responsible for the positive effects. We examined the portal vein transport of NEFA and other lipid related variables, in response to DAG and triacylglycerol (TAG) bolus feeding and a bolus of standard pig feed in 4 portal vein and mesenteric artery catheterized pigs. Also, the effect of the boluses on...

  10. Effect of periodontal treatment on serum C-reactive protein level in obese and normal-weight women affected with chronic periodontitis

    NARCIS (Netherlands)

    Al-Zahrani, M.S.; Alghamdi, H.S.

    2012-01-01

    OBJECTIVE: To investigate the effect of conventional periodontal therapy on serum C-reactive protein (CRP) level and periodontal status in obese and normal-weight chronic periodontitis patients. METHODS: This is a controlled clinical trial conducted at the King Abdulaziz University Faculty of Dentis

  11. Correlation of serum lithium levels and thyroid function tests in subjects of bipolar affective disorder: a prospective hospital-based study

    Directory of Open Access Journals (Sweden)

    M. S. Manjuprasad

    2015-02-01

    Conclusions: As already known, Lithium is a drug of narrow therapeutic index and females are more prone for thyroid function abnormalities. Appropriate monitoring of serum lithium levels will aid in necessary dose adjustment and ensure proper utilization of drug. [Int J Basic Clin Pharmacol 2015; 4(1.000: 51-54

  12. Patterns of serum PCDD/Fs affected by vegetarian regime, consumption of locally produced food, and resident places of residents living near incinerators

    Energy Technology Data Exchange (ETDEWEB)

    Chen Hsiu-Ling; Lee Chang-Ching; Su Hue-Jen; Liao Pao-Chi [Inst. of Basic Medical Science, Medical Coll., National Cheng Kung Univ., Tainan (Taiwan)

    2004-09-15

    Previous reports have been estimated that more than 90% of serum PCDD/Fs can be accounted for by the consumption of various food groups. Nouwen et al. suggested residents living near incinerators with the elevated dioxin exposure while they ate locally produced food compared to the general population from other areas. PCDD/Fs usually abound in fatty meats and marine foods, and consumption of which is the major pathway of human expose to PCDD/Fs. However, no distinct association was shown between vegetarian regime of human and their corresponding serum PCDD/Fs concentration. In addition, the resident region is associated to their corresponding dietary consumption and ambient exposure of PCDD/Fs, especially for subjects consumed the local foods with special dioxin-like contamination. The current study, therefore, was set to examine how dietary habits, including vegetarian regime and consumption of local food original for residents living near the incinerators are associated to serum PCDD/F concentrations. In addition, the further aim is to assess the influence on the serum PCDD/Fs levels resulting from subjects living in the alternative resident regions, even all of them exposed to PCDD/Fs emission from incinerators.

  13. Alterations of lipid metabolism in Wilson disease

    OpenAIRE

    Stremmel Wolfgang; Eckert Nicola; Pfeiffenberger Jan; Gotthardt Daniel; Gohdes Annina; Seessle Jessica; Reuner Ulrike; Weiss Karl

    2011-01-01

    Abstract Introduction Wilson disease (WD) is an inherited disorder of human copper metabolism, characterised by accumulation of copper predominantly in the liver and brain, leading to severe hepatic and neurological disease. Interesting findings in animal models of WD (Atp7b-/- and LEC rats) showed altered lipid metabolism with a decrease in the amount of triglycerides and cholesterol in the serum. However, serum lipid profile has not been investigated in large human WD patient cohorts to dat...

  14. Fibroblast growth factor 21 is elevated in metabolically unhealthy obesity and affects lipid deposition, adipogenesis, and adipokine secretion of human abdominal subcutaneous adipocytes

    Directory of Open Access Journals (Sweden)

    Lucia Berti

    2015-07-01

    Conclusions: The hepatokine FGF21 exerts weak lipogenic and anti-adipogenic actions and marked adiponectin-suppressive and leptin and interleukin-6 release-promoting effects in human differentiating preadipocytes. Together with the higher serum concentrations in MUHO subjects, our findings reveal FGF21 as a circulating factor promoting the development of metabolically unhealthy adipocytes.

  15. PolyCystic Ovary Syndrome in Monozygotic Twins Concordant for Lipid Profiles in Iranian Reproductive Women

    Directory of Open Access Journals (Sweden)

    Leila Amini

    2014-04-01

    Full Text Available Objective: There is evidence for genetics components in PCOS based on familial clustering of cases. We examined the contribution of genetics impact and some lipid/serum profiles to PCOS in a cohort study of Iranian twins. Materials and Methods: As a prominent predisposing risk factor for PCOS, serum lipid profiles (Triglyceride, Total cholesterol, HDL, LDL, Apo lipoprotein A–I, Apo lipoprotein B and lipoprotein A, were analyzed in 154 subjects of PCOS. The diagnosis of PCOS has been based on the Rotterdam consensus criteria in this study. We also studied 16 identical twin pairs discordant for PCOS to evaluate the effects of environmental risk factors (including lipid indices profiles for PCOS. Furthermore, we compared these 16 twin pairs with PCOS, in which just 15 (93.75% of them was concordant for abnormal lipid profiles. Also the impact of lipid profile in patients with PCOS was confirmed by comparing total 22 identical and non-identical twin pairs, in which 21 out of them (95.45% showed abnormal lipid profiles. Result: PCOS and increased cholesterol level were higher in identical versus non-identical twins with concordance rates of 1.5 and 1.65 (based on the Rotterdam consensus criteria, respectively. Analysis of identical pairs showed weak association between impact of lipid profile and PCOS while in non-identical pairs, the PCOS was associated with an increased environmental risk (lipid profile. Conclusion: Different lipid indices were affected by environmental and genetic risk factors in PCOS, but the contribution of environmental and genetic factors to the various PCOS-related changes in ovarian structures remains to be established.

  16. Changes in the hormone and lipid profile of obese adolescent Saudi females with acne vulgaris

    OpenAIRE

    K. O. Abulnaja

    2009-01-01

    Acne vulgaris is a multifactorial disease affecting a majority of the adolescent population. The objective of this study was to test for a correlation between fasting serum lipid profiles and levels of testosterone, insulin, leptin, and interleukin 1-β (IL-1β) and the incidence of severe acne vulgaris in obese adolescent females. Four groups of adolescent females were studied: obese with acne, obese without acne, non-obese with acne, and non-obese without acne. Obese females with ac...

  17. ACUTE PHASE PROTEINS, LIPID PROFILE AND PROINFLAMMATORY CYTOKINES IN HEALTHY AND BRONCHOPNEUMONIC WATER BUFFALO CALVES

    OpenAIRE

    El-Bahr, Sabry M.; Wael M. EL-Deeb

    2013-01-01

    The aim of the present study was to evaluate the diagnostic value of Acute Phase Proteins (APP), lipid profiles and proinflammatory cytokines in healthy and bronchopneumonic water buffalo calves. Therefore, sixty water buffalo calves (9±1 month old, 175±15 kg) were divided into two equal groups, the first group represented healthy, control, calves whereas calves of the second group were affected with bronchopneumonia. Total leukocytic and differential counts were determined. Serum total pr...

  18. The effects of lipids on channel function

    OpenAIRE

    Lee, Anthony G.

    2009-01-01

    Anionic lipids affect the function of many channels, including connexins, as shown in a recent report in BMC Biology. These effects might follow from direct binding of the anionic lipids to the channels.

  19. Characterization of the Aroma-Active, Phenolic, and Lipid Profiles of the Pistachio (Pistacia vera L.) Nut as Affected by the Single and Double Roasting Process.

    Science.gov (United States)

    Rodríguez-Bencomo, Juan José; Kelebek, Hasim; Sonmezdag, Ahmet Salih; Rodríguez-Alcalá, Luis Miguel; Fontecha, Javier; Selli, Serkan

    2015-09-01

    The pistachio (Pistacia vera L.) nut is one of the most widely consumed edible nuts in the world. However, it is the roasting process that makes the pistachio commercially viable and valuable as it serves as the key step to improving the nut's hallmark sensory characteristics including flavor, color, and texture. Consequently, the present study explores the effects of the single-roasting and double-roasting process on the pistachio's chemical composition, specifically aroma-active compounds, polyphenols, and lipids. Results showed the total polyphenol content of increased with the roasting treatment; however, not all phenolic compounds demonstrated this behavior. With regard to the aroma and aroma-active compounds, the results indicated that roasting process results in the development of characteristics and pleasant aroma of pistachio samples due to the Maillard reaction. With regard to lipids, the pistachio roasting treatment reduced the concentration of CN38 diacylglycerides while increasing the amount of elaidic acid. PMID:26301818

  20. Green tea powder and Lactobacillus plantarum affect gut microbiota, lipid metabolism and inflammation in high-fat fed C57BL/6J mice

    OpenAIRE

    Axling Ulrika; Olsson Crister; Xu Jie; Fernandez Céline; Larsson Sara; Ström Kristoffer; Ahrné Siv; Holm Cecilia; Molin Göran; Berger Karin

    2012-01-01

    Abstract Background Type 2 diabetes is associated with obesity, ectopic lipid accumulation and low-grade inflammation. A dysfunctional gut microbiota has been suggested to participate in the pathogenesis of the disease. Green tea is rich in polyphenols and has previously been shown to exert beneficial metabolic effects. Lactobacillus plantarum has the ability to metabolize phenolic acids. The health promoting effect of whole green tea powder as a prebiotic compound has not been thoroughly inv...

  1. Concentración de insulina y lípidos séricos en adolescentes de preparatoria en Guadalajara, México Serum concentrations of insulin and lipids among highschool adolescents in Guadalajara, Mexico

    Directory of Open Access Journals (Sweden)

    2003-01-01

    Full Text Available OBJETIVO: Determinar las concentraciones de insulina y lípidos en adolescentes mexicanos de 14 a 19 años según edad, género y nivel socioeconómico. MATERIAL Y MÉTODOS: Se realizó un estudio transversal en 352 adolescentes de entre 14 y 19 años de edad, estudiantes de bachillerato en la ciudad de Guadalajara, México. El estudio se efectuó de octubre de 1998 a marzo de 1999. Se midió insulina, colesterol total, triglicéridos, lipoproteínas de alta (HDL, baja (LDL y muy baja (VLDL densidad. Se utilizaron pruebas t de Student, ji cuadrada y ANOVA para hacer comparaciones entre medias y proporciones. RESULTADOS: La media de insulina fue 54±30 pmol/l; triglicéridos 101±36 mg/dl; colesterol total, 157±27 mg/dl; LDL 95±26 mg/dl y HDL 44±9 mg/dl. CONCLUSIONES: Las mujeres tuvieron concentraciones de colesterol total, LDL y HDL más altos que los hombres. La HDL fue menor en el nivel socioeconómico bajo. La insulina fue más alta en adolescentes más jóvenes.OBJECTIVE: To determine the serum concentration of insulin and lipids among highschool students, according to age, sex, and socioeconomic level. MATERIAL AND METHODS: A cross-sectional study was carried out between October 1998 and March 1999 among 352 high school students aged 14-19 years in Guadalajara, Mexico. Insulin, total cholesterol, triglyceride, high-density lipoprotein (HDL, low density lipoprotein (LDL, and very low density lipoprotein (VLDL levels were measured. Student's t test, chi- squared tests, and ANOVA, were used to compare mean measurements and proportions. RESULTS: Mean values were obtained for insulin (54±30 pmol/l; triglycerides (101±36 mg/dl; total cholesterol (157±27 mg/dl; LDL (95±26 mg/dl, and HDL (44±9 mg/dl. CONCLUSIONS: Total cholesterol, LDL, and HDL levels were higher among females. Hol levels were lower in low socioeconomic level. Insulin levels were higher among younger subjects.

  2. 血脂代谢紊乱与中青年脑梗死危险因素的相关性分析%The relationship between serum lipid metabolic dysfunction and cerebral infarction in middle age and youth

    Institute of Scientific and Technical Information of China (English)

    吴军; 赵水平; 郭纯; 蒋波; 谭利明; 肖志杰

    2004-01-01

    目的:探讨血脂与中青年人脑梗死的关系. 方法:检测了 111例中青年人脑梗死患者及 80例对照者的三酰甘油、总胆固醇、高密度脂蛋白胆固醇 (High density lipoprotein cholesterol, HDL-C)、低密度脂蛋白胆固醇 (Low density lipoprotein cholesterol,LDL-C)、载脂蛋白 A-I(apolipoproteinA-I,ApoA-I)、载脂蛋白 B100(apolipoprotein B100, ApoB100)和脂蛋白 (a)血清含量. 结果:脑梗死组三酰甘油 [(1.92± 1.33)mmol/L],总胆固醇 [(5.21± 1.08)mmol/L],LDL-C[(3.13± 0.96)]mmol/L,ApoB100[(1.10± 0.29)g/L]及脂蛋白 (a)[(0.23± 0.18)]g/L水平高于对照组 (t=2.523~ 3.796,P< 0.05),总胆固醇与年龄呈正相关 [青年: (4.96± 1.14)mmol/L,中年 :(5.27± 1.06)mmol/L], HDL-C与年龄呈负相关 [青年: (1.39± 0.43)mmol/L,中年 :(1.26± 0.35)mmol/L].亚组分析发现青年人脑梗死亚组的脂蛋白 (a)水平和中年人脑梗死亚组的三酰甘油、总胆固醇、 LDL-C、 ApoB100及脂蛋白 (a)水平均显著高于相应的对照组 (t=2.571~ 4.107,P< 0.05);皮层支动脉闭塞亚组脂蛋白 (a)水平显著高于穿通支动脉闭塞亚组 (t=5.414,P< 0.01);首发脑梗死亚组与复发脑梗死亚组之间的血脂水平无显著差异. 结论:血脂代谢紊乱是中青年人脑梗死的危险因素.%AIM:To explore the relationship between serum lipid and cerebral infarction in middle age and youth. METHODS:Blood samples were collected from 111 middle-aged and young patients with cerebral infarction and 80 normal controls for assessing the contents of triacylglycerol(TG), total cholesterol(TC), high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),apoliprotein A-1(ApoA-1),apoliprotein B100 (ApoB100) and lipoprotein(a). RESULTS:The levels of TG,TC,LDL-C,ApoB100 and lipoprotein(a) in the patients with cerebral infarction were higher than those of the control subjects (t=2.523- 3.796,P< 0.05);positive correlation was found between TC and age[(4.96

  3. Milk lipids

    Science.gov (United States)

    Milk fat conveys a number of desirable qualities to food, and various lipid components contribute to human nutrition and health. Over 96% of milk lipids consist of triacylglycerols, which contain a variety of fatty acids. Di- and monoacylglycerols, free fatty acids, sterols, and phospho-, glyco-,...

  4. Physiologic cold shock of Moraxella catarrhalis affects the expression of genes involved in the iron acquisition, serum resistance and immune evasion

    Directory of Open Access Journals (Sweden)

    Schaller André

    2011-08-01

    Full Text Available Abstract Background Moraxella catarrhalis, a major nasopharyngeal pathogen of the human respiratory tract, is exposed to rapid downshifts of environmental temperature when humans breathe cold air. It was previously shown that the prevalence of pharyngeal colonization and respiratory tract infections caused by M. catarrhalis are greatest in winter. The aim of this study was to investigate how M. catarrhalis uses the physiologic exposure to cold air to upregulate pivotal survival systems in the pharynx that may contribute to M. catarrhalis virulence. Results A 26°C cold shock induces the expression of genes involved in transferrin and lactoferrin acquisition, and enhances binding of these proteins on the surface of M. catarrhalis. Exposure of M. catarrhalis to 26°C upregulates the expression of UspA2, a major outer membrane protein involved in serum resistance, leading to improved binding of vitronectin which neutralizes the lethal effect of human complement. In contrast, cold shock decreases the expression of Hemagglutinin, a major adhesin, which mediates B cell response, and reduces immunoglobulin D-binding on the surface of M. catarrhalis. Conclusion Cold shock of M. catarrhalis induces the expression of genes involved in iron acquisition, serum resistance and immune evasion. Thus, cold shock at a physiologically relevant temperature of 26°C induces in M. catarrhalis a complex of adaptive mechanisms that enables the bacterium to target their host cellular receptors or soluble effectors and may contribute to enhanced growth, colonization and virulence.

  5. Effect of dietary fructans and chromium(III supplementation on apparent fat digestibility and blood lipid indices in rat

    Directory of Open Access Journals (Sweden)

    Zbigniew Krejpcio

    2009-06-01

    Full Text Available Background. Fructans and chromium(III are believed to influence lipid and carbohydrate metabolism in animal and human organisms, that can be applied in regulation or improvement of lipid indices, however the importance of their effect is still unclear. This study aimed at evaluation of the effect of inulin-type fructans and chromium(III supplementation on fat digestibility and blood lipid indices in rats. Material and methods. In a three-factorial design 56 male Wistar rats were fed ad libitum semipurified diets (AIN-93 supplemented with two types and levels of fructans (inulin or oligofructose, 5% or 10% of diet and two levels of chromium(III (0.5 or 5 mg/kg of diet; as Cr(III propionate complex for 10 weeks. Apparent fat digestibility and blood lipid profile were determined. Results. It was found that experimental factors did not influence apparent fat digestibility, serum total cholesterol, HDL and HDL cholesterol concentrations, however serum TAG level was significantly lower in animals fed oligofructose vs. inulin diets, high-fructan vs. low-fructan diet. Conclusions. Inulin type fructans and supplementary Cr(III do not affect apparent fat digestibility, fructans however influence lipid metabolism, lowering serum TAG levels in rat.

  6. Reduction of serum IGF-I levels in patients affected with Monoclonal Gammopathies of undetermined significance or Multiple Myeloma. Comparison with bFGF, VEGF and K-ras gene mutation

    Directory of Open Access Journals (Sweden)

    Pisani Francesco

    2009-03-01

    Full Text Available Abstract Background Serum levels of IGF-I in patients affected with multiple myeloma (MM have been scarcely studied. The present study is aimed to explore this point comparing 55 healthy subjects, 71 monoclonal gammopaties of uncertain significance (MGUS and 77 overt MM patients. In the same subjects, basic FGF and VEGF, have been detected. All three mediators were analyzed in function of K-ras mutation and melphalan response. Concerning IGF-I, two representative monitoring examples have also been added. Methods Cytokine determinations were performed by commercially available ELISA kits, while K12-ras mutation was investigated on genomic DNA isolated from bone marrow cell specimens by RFLP-PCR assay. Results Significant reductions of IGF-I levels were observed in MGUS and MM as compared with healthy controls. In addition, MM subjects showed significantly decreased serum IGF-I levels than MGUS. Conversely, increasing levels were observed for bFGF and VEGF, molecules significantly correlated. A multivariate analysis corrected for age and gender confirmed the significant difference only for IGF-I values (P = 0.01. K12-ras mutation was significantly associated with malignancy, response to therapy and with significantly increased serum bFGF levels. Conclusion IGF-I reduction in the transition: Controls→MGUS→MM and changes observed over time suggest that IGF-I should be furtherly studied in future clinical trials as a possible monitoring marker for MM.

  7. Haplotypes in the APOA1-C3-A4-A5 gene cluster affect plasma lipids in both humans and baboons

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Qian-fei; Liu, Xin; O' Connell, Jeff; Peng, Ze; Krauss, Ronald M.; Rainwater, David L.; VandeBerg, John L.; Rubin, Edward M.; Cheng, Jan-Fang; Pennacchio, Len A.

    2003-09-15

    Genetic studies in non-human primates serve as a potential strategy for identifying genomic intervals where polymorphisms impact upon human disease-related phenotypes. It remains unclear, however, whether independently arising polymorphisms in orthologous regions of non-human primates leads to similar variation in a quantitative trait found in both species. To explore this paradigm, we studied a baboon apolipoprotein gene cluster (APOA1/C3/A4/A5) for which the human gene orthologs have well established roles in influencing plasma HDL-cholesterol and triglyceride concentrations. Our extensive polymorphism analysis of this 68 kb gene cluster in 96 pedigreed baboons identified several haplotype blocks each with limited diversity, consistent with haplotype findings in humans. To determine whether baboons, like humans, also have particular haplotypes associated with lipid phenotypes, we genotyped 634 well characterized baboons using 16 haplotype tagging SNPs. Genetic analysis of single SNPs, as well as haplotypes, revealed an association of APOA5 and APOC3 variants with HDL cholesterol and triglyceride concentrations, respectively. Thus, independent variation in orthologous genomic intervals does associate with similar quantitative lipid traits in both species, supporting the possibility of uncovering human QTL genes in a highly controlled non-human primate model.

  8. Efeito da dieta hipoenergética sobre a composição corporal e nível sérico lipídico de mulheres adultas com sobrepeso Effect of a low-energy diet on the body composition and serum lipid levels of overweight adult women

    Directory of Open Access Journals (Sweden)

    Valéria Sales do Valle

    2010-12-01

    Full Text Available OBJETIVO: Verificar o efeito de 12 semanas de dieta hipocalórica sobre a composição corporal e o nível sérico lipídico de mulheres adultas com sobrepeso. MÉTODOS: A amostra foi composta por vinte mulheres (23,80, desvio-padrão de 2,73 anos da academia Westfit-Bangu, divididas randomicamente em dois grupos de dez: grupo controle e grupo dieta hipoenergética. Foi realizada uma avaliação da composição corporal (massa corporal, percentual de gordura, índice de massa corporal e massa magra e do nível sérico lipídico (colesterol total, triglicerídeos, lipoproteína de baixa densidade, lipoproteína de alta densidade e lipoproteínas de muito baixa densidade. Utilizou-se a estatística descritiva (média e desvio-padrão e o teste t de Student na análise inter e intragrupos. O nível de significância foi de pOBJECTIVE: This study verified the effects of a 12-week low-calorie diet on the body composition and serum lipid levels of overweight adult women. METHODS: The sample consisted of 20 females (23.80 years with a standard deviation of 2.73 years of the Westfit-Bangu gym. They were randomly divided into two groups of ten: the Control Group and the Diet Group. Body composition (body mass, %fat, body mass index and lean body mass and serum lipid levels (total cholesterol, triglycerides and high-, low- and very low-density lipoproteins were assessed. Descriptive (central tendency and dispersion and inferential statistics (Student's t-test were used for inter and intragroup analysis. The significance level was set at p<0.05. RESULTS: The Diet Group presented a significant decrease (p<0.05 in anthropometric variables (body mass, % of fat, BMI and lean mass and serum lipids (triglycerides, total cholesterol and low- and very low-density lipoproteins. The level of high-density lipoprotein also decreased but not significantly. CONCLUSION: In conclusion, the low-calorie diet was an excellent option for the treatment of obesity and to

  9. Lipid metabolism in mitochondrial membranes.

    Science.gov (United States)

    Mayr, Johannes A

    2015-01-01

    Mitochondrial membranes have a unique lipid composition necessary for proper shape and function of the organelle. Mitochondrial lipid metabolism involves biosynthesis of the phospholipids phosphatidylethanolamine, cardiolipin and phosphatidylglycerol, the latter is a precursor of the late endosomal lipid bis(monoacylglycero)phosphate. It also includes mitochondrial fatty acid synthesis necessary for the formation of the lipid cofactor lipoic acid. Furthermore the synthesis of coenzyme Q takes place in mitochondria as well as essential parts of the steroid and vitamin D metabolism. Lipid transport and remodelling, which are necessary for tailoring and maintaining specific membrane properties, are just partially unravelled. Mitochondrial lipids are involved in organelle maintenance, fission and fusion, mitophagy and cytochrome c-mediated apoptosis. Mutations in TAZ, SERAC1 and AGK affect mitochondrial phospholipid metabolism and cause Barth syndrome, MEGDEL and Sengers syndrome, respectively. In these disorders an abnormal mitochondrial energy metabolism was found, which seems to be due to disturbed protein-lipid interactions, affecting especially enzymes of the oxidative phosphorylation. Since a growing number of enzymes and transport processes are recognised as parts of the mitochondrial lipid metabolism, a further increase of lipid-related disorders can be expected. PMID:25082432

  10. Lipid oxidation in fish oil enriched oil-in-water emulsions and cream cheese with pre-emulsified fish oil is affected differently by the emulsifier used

    DEFF Research Database (Denmark)

    Horn, Anna Frisenfeldt; Nielsen, Nina Skall; Andersen, Ulf;

    emulsifier. The antioxidative properties of milk proteins make them an obvious choice as emulsifiers in delivery emulsions. Previous studies have furthermore shown that a combination of proteins and phospholipids may increase the thickness of the interfacial layer in an emulsion. This presentation will...... include results from studies on lipid oxidation in simple oil-in-water emulsions prepared with milk proteins alone or combinations of milk proteins and phospholipids. In addition, a study on fish oil enriched cream cheese will be presented. In this study, the cream cheese was enriched with either neat...... fish oil or a fish oil-in-water delivery emulsion prepared with whey protein isolate, sodium caseinate or a commercially available emulsifier that consisted of ~20% milk phospholipids and ~50% milk proteins. Results showed that simple emulsions prepared with a combination of milk proteins and...

  11. Green tea powder and Lactobacillus plantarum affect gut microbiota, lipid metabolism and inflammation in high-fat fed C57BL/6J mice

    Directory of Open Access Journals (Sweden)

    Axling Ulrika

    2012-11-01

    Full Text Available Abstract Background Type 2 diabetes is associated with obesity, ectopic lipid accumulation and low-grade inflammation. A dysfunctional gut microbiota has been suggested to participate in the pathogenesis of the disease. Green tea is rich in polyphenols and has previously been shown to exert beneficial metabolic effects. Lactobacillus plantarum has the ability to metabolize phenolic acids. The health promoting effect of whole green tea powder as a prebiotic compound has not been thoroughly investigated previously. Methods C57BL/6J mice were fed a high-fat diet with or without a supplement of 4% green tea powder (GT, and offered drinking water supplemented with Lactobacillus plantarum DSM 15313 (Lp or the combination of both (Lp + GT for 22 weeks. Parameters related to obesity, glucose tolerance, lipid metabolism, hepatic steatosis and inflammation were examined. Small intestinal tissue and caecal content were collected for bacterial analysis. Results Mice in the Lp + GT group had significantly more Lactobacillus and higher diversity of bacteria in the intestine compared to both mice in the control and the GT group. Green tea strongly reduced the body fat content and hepatic triacylglycerol and cholesterol accumulation. The reduction was negatively correlated to the amount of Akkermansia and/or the total amount of bacteria in the small intestine. Markers of inflammation were reduced in the Lp + GT group compared to control. PLS analysis of correlations between the microbiota and the metabolic variables of the individual mice showed that relatively few components of the microbiota had high impact on the correlation model. Conclusions Green tea powder in combination with a single strain of Lactobacillus plantarum was able to promote growth of Lactobacillus in the intestine and to attenuate high fat diet-induced inflammation. In addition, a component of the microbiota, Akkermansia, correlated negatively with several metabolic parameters

  12. Increasing the stearate content in seed oil of Brassica juncea by heterologous expression of MlFatB affects lipid content and germination frequency of transgenic seeds.

    Science.gov (United States)

    Bhattacharya, Surajit; Sinha, Saheli; Das, Natasha; Maiti, Mrinal K

    2015-11-01

    Fatty acids from dietary lipids can impart both beneficial and harmful health effects. The compositional balance between saturated and unsaturated fatty acids plays a decisive role in maintaining the physiological harmony, proper growth and development in the human system. In case of Brassica juncea seed oil, the level of saturated fatty acid, especially desirable stearate is very much lower than the recommended value, along with a high content of nutritionally undesirable erucic acid. Therefore, in order to shift the carbon flux towards the production of stearate at the expense of erucate, the MlFatB gene encoding a FatB thioesterase from Madhuca longifolia (latifolia) was expressed heterologously in seed tissues of B. juncea. The functional MlFatB competed with the highly active endogenous BjFatA thioesterase, and the transgenic B. juncea lines showed noteworthy changes in their seed fatty acid profiles. The proportion of stearate increased up to 16-fold, constituting almost 31% of the total fatty acids along with the production of arachidic acid in significant amount (up to ∼11%). Moreover, the content of erucate was reduced up to 71% in the seed oils of transgenic lines. Although a nutritionally desirable fatty acid profile was achieved, the transgenic seeds exhibit reduction or abolition of seed germination in addition to a decrease in seed lipid content. The findings of the present study revealing the stearoyl-ACP thioesterase-mediated enhancement of the stearate content that is associated with reduced germination frequency of transgenic B. juncea seeds, may explain why no natural or induced stearate-rich Brassica has been found or developed. Furthermore, this study also suggests that the newly characterized MlFatB is a potential candidate gene for refined metabolic engineering strategy in B. juncea or other plant species for increasing stearate content in seed oil. PMID:26351151

  13. Effects of pork vs veal consumption on serum lipids in healthy subjects Efecto del consumo de cerdo y de ternera sobre el perfil lipídico de sujetos sanos

    OpenAIRE

    Rubio, J.A.; Rubio, M A; L. Cabrerizo; P. Burdaspal; R. Carretero; Gómez-Gerique, J.A.; Montoya, M T; M. L. Maestro; Sanz, M. T.; Fernández, C.

    2006-01-01

    AIMS: To analyse the influence of lean pork (P) and veal (V) consumption on the lipid profile of healthy subjects within the framework of a healthy diet comprising low levels of total fat (TF), saturated fatty acids (SFA) and cholesterol. DESIGN: Double-crossover, randomized and controlled trial SUBJECTS: 44 healthy individuals (22 male and 22 female), recruited voluntarily from the University Complutense of Madrid. The weight and lipid profiles of these volunteers were normal and their dieta...

  14. Effect of Z Mixture and Exercise Swimmise on the Levels of Serum Lipids in Rats%游泳训练和中药对大鼠血脂含量的影响

    Institute of Scientific and Technical Information of China (English)

    付毅; 霍科科; 贺国侠; 余京新

    2001-01-01

    Objective The purposeof this investigation was to measure the changes of Seuumlipid levels in rats. Method Forty male SD rats were radomly divided into four groups: control, drug, exercise and diug + exercise. The exercise and drug + exercise performed swimmingexercise training progrm(5 days/week, 1.5 hour/day)a 8weeks. Result (1)The weights of the rats were significantly lower in drug + exercisegroups: (2) TC, TG, LDL - c and AL levels were decreased and HDL - c increased in drug, exercise and drug + exercise groups; in drug + ex-ercise group, HDL -c was obviously increaséd( P<0.01) ascompared with control group. (3) ApoB-100 levels significantly decreased indrug, exercise and drug + exercise groups( P <0.01 ); ApoAI and ApoA/ApoB increased in drug and drug + exercise Groups( P < 0. 05);ApoB - 100 decreased and ApoAI, ApoA/ApoB, increased in drug + exercise group( P < 0. 05 ). Conclusion There results suggeasted thatZiDanLan and exercise have a significant effect on controlling weight and regu]ating the metabolism of serum lipids and may be beneficial toantiatherosclerosis.%目的测定中年大鼠血脂含量的变化。方法40只雄性SD大鼠随机分为四组:对照组、服药组、运动组和服药+运动组。后二组进行游泳训练8周(5d/周,1.5h/d)。结果(1)服药+运动组体重明显下降。(2)与对照组相比,服药组、运动组和服药+运动组血清TC、TG、LDL-C和动脉粥样硬化指数AI降低,HDL-C提高;服药+运动组HDL-C提高有极显著差异(P<0.01)。(3)与对照组相比,后三组APOB-100极显著降低(P<0.01);服药组、服药+运动组APOAI和APOA/APOB比值显著提高(P<0.05);服药+运动组和运动组相比,APOB-100降低和APOAI、APOA/APOB提高有显著差异(P<0.05)。结论中药紫丹蓝和运动训练在调节脂代谢及抗动脉粥样硬化方面具有显著作用。

  15. Associations among serum pro- and anti-inflammatory cytokines, metabolic mediators, body condition, and uterine disease in postpartum dairy cows

    OpenAIRE

    Kasimanickam, Ramanathan K.; Kasimanickam, Vanmathy R; Olsen, Jesse R; Jeffress, Erin J; Moore, Dale A; Kastelic, John P.

    2013-01-01

    Background Adipose tissue is an active endocrine organ which secretes a w