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Sample records for affect production reproductive

  1. Reproductive biology traits affecting productivity of sour cherry

    Directory of Open Access Journals (Sweden)

    Milica Fotiric Aksic

    2013-01-01

    Full Text Available The objective of this work was to evaluate variability in reproductive biology traits and the correlation between them in genotypes of 'Oblačinska' sour cherry (Prunus cerasus. High genetic diversity was found in the 41 evaluated genotypes, and significant differences were observed among them for all studied traits: flowering time, pollen germination, number of fruiting branches, production of flower and fruit, number of flowers per bud, fruit set, and limb yield efficiency. The number of fruiting branches significantly influenced the number of flower and fruit, fruit set, and yield efficiency. In addition to number of fruiting branches, yield efficiency was positively correlated with fruit set and production of flower and fruit. Results from principal component analysis suggested a reduction of the reproductive biology factors affecting yield to four main characters: number and structure of fruiting branches, flowering time, and pollen germination. Knowledge of the reproductive biology of the 'Oblačinska' genotypes can be used to select the appropriate ones to be grown or used as parents in breeding programs. In this sense, genotypes II/2, III/9, III/13, and III/14 have very good flower production and satisfactory pollen germination.

  2. Divergence for residual feed intake of Holstein-Friesian cattle during growth did not affect production and reproduction during lactation.

    Science.gov (United States)

    Macdonald, K A; Thomson, B P; Waghorn, G C

    2016-11-01

    Residual feed intake (RFI) is the difference between actual and predicted dry matter intake (DMI) of individual animals. Recent studies with Holstein-Friesian calves have identified an ~20% difference in RFI during growth (calf RFI) and these groups remained divergent in RFI during lactation. The objective of the experiment described here was to determine if cows selected for divergent RFI as calves differed in milk production, reproduction or in the profiles of BW and body condition score (BCS) change during lactation, when grazing pasture. The cows used in the experiment (n=126) had an RFI of -0.88 and +0.75 kg DM intake/day for growth as calves (efficient and inefficient calf RFI groups, respectively) and were intensively grazed at four stocking rates (SR) of 2.2, 2.6, 3.1 and 3.6 cows/ha on self-contained farmlets, over 3 years. Each SR treatment had equal number of cows identified as low and high calf RFI, with 24, 28, 34 and 40/11 ha farmlet. The cows divergent for calf RFI were randomly allocated to each SR. Although SR affected production, calf RFI group (low or high) did not affect milk production, reproduction, BW, BCS or changes in these parameters throughout lactation. The most efficient animals (low calf RFI) lost similar BW and BCS as the least efficient (high calf RFI) immediately post-calving, and regained similar BW and BCS before their next calving. These results indicate that selection for RFI as calves to increase efficiency of feed utilisation did not negatively affect farm productivity variables (milk production, BCS, BW and reproduction) as adults when managed under an intensive pastoral grazing system.

  3. Pollen production in sunflower (Helianthus annuus L.) is affected by air temperature and relative humidity during early reproductive growth

    OpenAIRE

    Astiz, V.; Hernández, Luis Francisco

    2013-01-01

    During microsporogenesis, sunflower florets might be exposed to episodes of day- and night-time temperatures exceeding 30 and 20 °C, respectively, that can affect pollen availability and consequently plant’s yield. The relationship between air temperature and relative humidity during the meiotic phase of microsporogenesis, and the quantity and quality of pollen produced by the flowers, were studied in two sunflower hybrids during two years. The hybrids were grown on irrigated plots in Bahía B...

  4. Productive or re-productive learning?

    DEFF Research Database (Denmark)

    Nordentoft, Helle Merete; Olesen, Birgitte Ravn

    .e. differences such as level of education, gender, ethnic background and knowledge. However, after analyses of the data we wonder if the learning was more re-productive than productive. Our analysis shows how the majority voice, represented by Danish female participants, with its cultural preferences...

  5. Reproductive biology traits affecting productivity of sour cherry Características da biologia reprodutiva que afetam a produtividade de cereja ácida

    Directory of Open Access Journals (Sweden)

    Milica Fotiric Aksic

    2013-01-01

    Full Text Available The objective of this work was to evaluate variability in reproductive biology traits and the correlation between them in genotypes of 'Oblačinska' sour cherry (Prunus cerasus. High genetic diversity was found in the 41 evaluated genotypes, and significant differences were observed among them for all studied traits: flowering time, pollen germination, number of fruiting branches, production of flower and fruit, number of flowers per bud, fruit set, and limb yield efficiency. The number of fruiting branches significantly influenced the number of flower and fruit, fruit set, and yield efficiency. In addition to number of fruiting branches, yield efficiency was positively correlated with fruit set and production of flower and fruit. Results from principal component analysis suggested a reduction of the reproductive biology factors affecting yield to four main characters: number and structure of fruiting branches, flowering time, and pollen germination. Knowledge of the reproductive biology of the 'Oblačinska' genotypes can be used to select the appropriate ones to be grown or used as parents in breeding programs. In this sense, genotypes II/2, III/9, III/13, and III/14 have very good flower production and satisfactory pollen germination.O objetivo deste trabalho foi avaliar a variabilidade de características da biologia reprodutiva e a correlação entre elas, em genótipos da cereja ácida (Prunus cerasus 'Oblačinska'. Verificou-se alta diversidade genética nos 41 genótipos avaliados, e foram observadas diferenças significativas entre eles, para todas as características estudadas: época de florescimento, germinação de pólen, número de ramos frutíferos, produção de flores e frutos, número de flores por botão, formação de frutos e eficiência de produção dos ramos. O número de ramos frutíferos influenciou significativamente o número de flores e frutos, a formação de frutos e a eficiência produtiva. Além do número de

  6. Factors affecting the reproductive success of dominant male meerkats.

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    Spong, Göran F; Hodge, Sarah J; Young, Andrew J; Clutton-Brock, Tim H

    2008-05-01

    Identifying traits that affect the reproductive success of individuals is fundamental for our understanding of evolutionary processes. In cooperative breeders, a dominant male typically restricts mating access to the dominant female for extended periods, resulting in pronounced variation in reproductive success among males. This may result in strong selection for traits that increase the likelihood of dominance acquisition, dominance retention and reproductive rates while dominant. However, despite considerable research on reproductive skew, few studies have explored the factors that influence these three processes among males in cooperative species. Here we use genetic, behavioural and demographic data to investigate the factors affecting reproductive success in dominant male meerkats (Suricata suricatta). Our data show that dominant males sire the majority of all offspring surviving to 1 year. A male's likelihood of becoming dominant is strongly influenced by age, but not by weight. Tenure length and reproductive rate, both important components of dominant male reproductive success, are largely affected by group size and composition, rather than individual traits. Dominant males in large groups have longer tenures, but after this effect is controlled, male tenure length also correlates negatively to the number of adult females in the group. Male reproductive rate also declines as the number of intra- and extra-group competitors increases. As the time spent in the dominant position and reproductive rate while dominant explain > 80% of the total variance in reproductive success, group composition thus has major implications for male reproductive success.

  7. Factors affecting the reproductive traits of Holstein cows

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    Daniella Flavia Vilas Boas

    2012-12-01

    Full Text Available For dairy cattle breeds, mainly the taurine ones, the selection emphasized for many years the increase in milk yields and, as a consequence, the adaptive and reproductive traits were negatively affected. The aim of this study was to verify the influence of genetic and environmental effects on the reproductive traits in a dairy herd selected for high milk production levels. The data set comprised 1,737 first lactations Holsteins cows of Agrindus Farm, located at Southeastern region of Brazil. The records of the following reproductive traits: calving to first heat interval (CFHI, calving to conception interval (CCI and first to second calving interval (FCI were analyzed as dependent variables by least squares method using GLM procedure (SAS. Linear models were considered including two production levels (1= less than 9,500 kg and 2= more than 9,501 kg of total milk yield, contemporary group (year and months calving, management group, sire of cow, and the sire used to breeding cows, as classificatory variables. As covariates were included for all traits the peak milk yield in lactation (linear effect, age at calving only for CFHI (linear and quadratic effects since this effect was not significant for other traits, and CFHI (linear effect only for FCI. The coefficients of determination represented 24%, 74% and 75%, respectively for CFHI, FCI and CCI models. Production level, peak milk yield and sire effects were significant (P<0.05 for all traits. The average estimated for high and low milk production level were 73 and 79 days, 500 and 601 days, 227 and 330 days for CFHI, FCI and CCI, respectively, suggesting that cows with higher genetic potential for milk had worse reproductive performance. Similarly, lactation peak showed significant effect (P<0.05 for all traits, suggesting higher peaks cows showed also poorer reproductive rates. Sire effect also was a variable that showed significant effect (P<0.01 for all traits, which means that there was

  8. Environmental color affects Nile tilapia reproduction

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    Volpato G.L.

    2004-01-01

    Full Text Available We investigated the effects of environmental color on the reproductive behavior of Nile tilapia, Oreochromis niloticus. Two environmental colors were tested by covering the aquarium (60 x 60 x 40 cm with white (12 groups or blue (13 groups cellophane and observing reproductive behavior in groups of 2 males (10.27 ± 0.45 cm and 3 females (10.78 ± 0.45 cm each. After assignment to the respective environmental color (similar luminosity = 100 to 120 Lux, the animals were observed until reproduction (identified by eggs in the female's mouth or up to 10 days after the first nest building. Photoperiod was from 6:00 h to 18:00 h every day. Food was offered in excess once a day and water quality was similar among aquaria. Daily observations were made at 8:00, 11:00, 14:00 and 17:00 h regarding: a latency to the first nest, b number of nests, c gravel weight removed (the male excavates the nest in the bottom of the aquarium, d nest area, and e mouthbrooding incubation (indication of reproduction. The proportion of reproducing fish was significantly higher (6 of 13 in the group exposed to the blue color compared the group exposed to the white color (1 of 12; Goodman's test of proportions. Moreover, males under blue light removed significantly larger masses of gravel (blue = 310.70 ± 343.50 g > white = 130.38 ± 102.70 g; P = 0.01 and constructed wider nests (blue = 207.93 ± 207.80 cm² > white = 97.68 ± 70.64 cm²; P = 0.03 than the control (white. The other parameters did not differ significantly between light conditions. We concluded that reproduction in the presence of blue light was more frequent and intense than in the presence of white light.

  9. Paternal smoking habits affect the reproductive life span of daughters

    DEFF Research Database (Denmark)

    Fukuda, Misao; Fukuda, Kiyomi; Shimizu, Takashi

    2011-01-01

    The present study assessed whether the smoking habits of fathers around the time of conception affected the period in which daughters experienced menstrual cycles (i.e., the reproductive life span). The study revealed that the smoking habits of the farther shortened the daughters' reproductive life...

  10. DEHP impairs zebrafish reproduction by affecting critical factors in oogenesis.

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    Oliana Carnevali

    Full Text Available Public concerns on phthalates distributions in the environment have been increasing since they can cause liver cancer, structural abnormalities and reduce sperm counts in male reproductive system. However, few data are actually available on the effects of Di-(2-ethylhexyl-phthalate (DEHP in female reproductive system. The aim of this study was to assess the impacts of DEHP on zebrafish oogenesis and embryo production. Female Danio rerio were exposed to environmentally relevant doses of DEHP and a significant decrease in ovulation and embryo production was observed. The effects of DEHP on several key regulators of oocyte maturation and ovulation including bone morphogenetic protein-15 (BMP15, luteinizing hormone receptor (LHR, membrane progesterone receptors (mPRs and cyclooxygenase (COX-2 (ptgs2 were determined by real time PCR. The expressions of BMP15 and mPR proteins were further determined by Western analyses to strengthen molecular findings. Moreover, plasma vitellogenin (vtg titers were assayed by an ELISA procedure to determine the estrogenic effects of DEHP and its effects on oocyte growth. A significant reduction of fecundity in fish exposed to DEHP was observed. The reduced reproductive capacity was associated with an increase in ovarian BMP15 levels. This rise, in turn, was concomitant with a significant reduction in LHR and mPRbeta levels. Finally, ptgs2 expression, the final trigger of ovulation, was also decreased by DEHP. By an in vitro maturation assay, the inhibitory effect of DEHP on germinal vesicle breakdown was further confirmed. In conclusion, DEHP affecting signals involved in oocyte growth (vtg, maturation (BMP15, LHR, mPRs, and ovulation (ptgs2, deeply impairs ovarian functions with serious consequences on embryo production. Since there is a significant genetic similarity between D.rerio and humans, the harmful effects observed at oocyte level may be relevant for further molecular studies on humans.

  11. Pesticide soil contamination mainly affects earthworm male reproductive parameters

    Institute of Scientific and Technical Information of China (English)

    EduardoBustos-Obregon; RogerIzigaGoicochea

    2002-01-01

    Aim:To explore the effect of exposure to commercial Parathion(Pc)on the reproductive parameters(sperm and cocoon production and genotoxicity on male germ cells),the survival,the body weight and the gross anatomical changes in Eisenia foetida.Methods:Three doses of Pc(1478,739and 444mg/kg of soil)and three thme intervals of exposure(5,15and30days)were used.Results:Alltreated amimals were affected.An acute genotoxic effect,revealed by DNAfragmentation(comet assay),was seen by 5days,Alterations in reproductive parameters were conspicuous in regard to the number of sperm,cocoons and worms born,and the histological observation of the gonads and seminal receptacles.In addition,the body weight and survival rate were decreased,Neuromuscular function was also affected.Conclusion:Earthworms are suitable bioindicators of chemical contamination of the soil,their advantage being their easy and economical handling.

  12. Caste ratios affect the reproductive output of social trematode colonies.

    Science.gov (United States)

    Kamiya, T; Poulin, R

    2013-03-01

    Intraspecific phenotypic diversification in social organisms often leads to formation of physical castes which are morphologically specialized for particular tasks within the colony. The optimal caste allocation theory argues that specialized morphological castes are efficient at specific tasks, and hence different caste ratios should affect the ergonomic efficiency, hence reproductive output of the colony. However, the reproductive output of different caste ratios has been documented in few species of insects with equivocal support for the theory. This study investigated whether the ratios of nonreproductive and reproductive morphs affect the reproductive output of a recently discovered social trematode, Philophthalmus sp., in which the nonreproductive members are hypothesized to be defensive specialists. A census of natural infections and a manipulative in vitro experiment demonstrated a positive association between the reproductive output of trematode colonies and the ratio of nonreproductive to reproductive morphs in the presence of an intra-host trematode competitor, Maritrema novaezealandensis. On the contrary, without the competitor, reproductive output was negatively associated with the proportion of nonreproductive castes in colonies. Our findings demonstrate for the first time a clear fitness benefit associated with the nonreproductive castes in the presence of a competitor while illustrating the cost of maintaining such morphs in noncompetitive situations. Although the proximate mechanisms controlling caste ratio remain unclear in this trematode system, this study supports the prediction that the fitness of colonies is influenced by the composition of specialized functional morphs in social organisms, suggesting a potential for adaptive shifts of caste ratios over evolutionary time.

  13. Productive and Re-Productive Thinking in Solving Insight Problems

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    Cunningham, J. Barton; MacGregor, James N.

    2014-01-01

    Many innovations in organizations result when people discover insightful solutions to problems. Insightful problem-solving was considered by Gestalt psychologists to be associated with productive, as opposed to re-productive, thinking. Productive thinking is characterized by shifts in perspective which allow the problem solver to consider new,…

  14. Reproductive interference between Rana dalmatina and Rana temporaria affects reproductive success in natural populations.

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    Hettyey, Attila; Vági, Balázs; Kovács, Tibor; Ujszegi, János; Katona, Patrik; Szederkényi, Márk; Pearman, Peter B; Griggio, Matteo; Hoi, Herbert

    2014-10-01

    Experimental evidence suggests that reproductive interference between heterospecifics can seriously affect individual fitness; support from field studies for such an effect has, however, remained scarce. We studied reproductive interference in 25 natural breeding ponds in an area where two ranid frogs, Rana dalmatina and Rana temporaria, co-occur. The breeding seasons of the two species usually overlap and males of both species are often found in amplexus with heterospecific females, even though matings between heterospecifics produce no viable offspring. We estimated species abundance ratios based on the number of clutches laid and evaluated fertilization success. In ponds with low spatial complexity and a species abundance ratio biased towards R. temporaria, the average fertilization success of R. dalmatina eggs decreased, while this relationship was not detectable in spatially more complex ponds. Fertilization success of R. temporaria did not decrease with increasing relative numbers of heterospecifics. This asymmetry in fitness effects of reproductive interference may be attributed to R. temporaria males being more competitive in scramble competition for females than R. dalmatina males. Our study is among the first to demonstrate that in natural breeding populations of vertebrates interference among heterospecifics has the potential to substantially lower reproductive success at the population level, which may in turn affect population dynamics.

  15. Productive or re-productive learning?

    DEFF Research Database (Denmark)

    Olesen, Birgitte Ravn; Nordentoft, Helle

    in a group of 4 professionals reflects on how h/she experienced empathy was enacted from his/her position in the play. The participants appear to be challenged by their interdisciplinary and personal differences and we explore how they negotiate different knowledge and power relations in their talk about...... empathy. Inspired by Bakhtin’s dialogical theory, we look at centripetal and centrifugal forces in participants’ talk and uncover the way in which the group members’ tolerance for emergence of multiple knowledge forms and experience of the phenomenon of empathy becomes tensional. Normally it is our...... experience that the role play is a productive learning method which has potential to transform normative conceptions of “how one should act” into situated and concrete interactions and thereby illuminate differences in and also tolerance for the way in which empathy is understood and enacted – i...

  16. Awassi sheep reproduction and milk production: review.

    Science.gov (United States)

    Talafha, Abdelsalam Q; Ababneh, Mohammed M

    2011-10-01

    Awassi is the local breed of sheep in Jordan and is the most important breed in the semi-arid regions of the near east countries. Awassi ram and ewe lambs reach puberty at around 8 and 9 months of age, respectively. The breeding season of Awassi ewes starts as early as April and lasts through September. After puberty, Awassi rams are sexually active throughout the year. The normal estrous cycle in Awassi ewes is 15-20 days (average 17 days). Estrus ranges from 16-59 h (average 29 h) during the breeding season. The reproductive performance of unimproved Awassi sheep has been low while improved Awassi has the highest fertility and milk production and are the heaviest among all Awassi populations. The gestation length varies from 149 to 155 days (average 152 days). Hormones that are commonly used for induction and synchronization of estrus in Awassi ewes include progestins, gonadotropins and PGF2α. An Awassi ewe produces 40-60 and 70-80 kg of milk per 150-day lactation period under traditional and improved production systems, respectively, in addition to the suckled milk left for lambs until weaning. The improved Awassi has the highest milk production among all Awassi populations and may reach 506 L over 214-day lactation period. The objective of this review is to summarize the reproductive pattern and milk production of Awassi sheep in the Middle East region.

  17. Ozone affects gas exchange, growth and reproductive development in Brassica campestris (Wisconsin fast plants).

    Science.gov (United States)

    Black, V J; Stewart, C A; Roberts, J A; Black, C R

    2007-01-01

    Exposure to ozone (O(3)) may affect vegetative and reproductive development, although the consequences for yield depend on the effectiveness of the compensatory processes induced. This study examined the impact on reproductive development of exposing Brassica campestris (Wisconsin Fast Plants) to ozone during vegetative growth. Plants were exposed to 70 ppb ozone for 2 d during late vegetative growth or 10 d spanning most of the vegetative phase. Effects on gas exchange, vegetative growth, reproductive development and seed yield were determined. Impacts on gas exchange and foliar injury were related to pre-exposure stomatal conductance. Exposure for 2 d had no effect on growth or reproductive characteristics, whereas 10-d exposure reduced vegetative growth and reproductive site number on the terminal raceme. Mature seed number and weight per pod and per plant were unaffected because seed abortion was reduced. The observation that mature seed yield per plant was unaffected by exposure during the vegetative phase, despite adverse effects on physiological, vegetative and reproductive processes, shows that indeterminate species such as B. campestris possess sufficient compensatory flexibility to avoid reductions in seed production.

  18. Sexual selection by cryptic female choice on male seminal products - a new bridge between sexual selection and reproductive physiology.

    Science.gov (United States)

    Eberhard, W G; Cordero, C

    1995-12-01

    Selection clearly focuses on differences in reproduction, but studies of reproductive physiology generally have been carried out in a near vacuum of modern evolutionary theory. This lack of contact between the two fields may be about to change. New ideas indicate that sexual selection by cryptic female choice has affected the evolution of products in male semen that influence female reproductive behavior and physiology.

  19. Male seminal fluid substances affect sperm competition success and female reproductive behavior in a seed beetle.

    Directory of Open Access Journals (Sweden)

    Takashi Yamane

    Full Text Available Male seminal fluid proteins are known to affect female reproductive behavior and physiology by reducing mating receptivity and by increasing egg production rates. Such substances are also though to increase the competitive fertilization success of males, but the empirical foundation for this tenet is restricted. Here, we examined the effects of injections of size-fractioned protein extracts from male reproductive organs on both male competitive fertilization success (i.e., P2 in double mating experiments and female reproduction in the seed beetle Callosobruchus maculatus. We found that extracts of male seminal vesicles and ejaculatory ducts increased competitive fertilization success when males mated with females 1 day after the females' initial mating, while extracts from accessory glands and testes increased competitive fertilization success when males mated with females 2 days after the females' initial mating. Moreover, different size fractions of seminal fluid proteins had distinct and partly antagonistic effects on male competitive fertilization success. Collectively, our experiments show that several different seminal fluid proteins, deriving from different parts in the male reproductive tract and of different molecular weight, affect male competitive fertilization success in C. maculatus. Our results highlight the diverse effects of seminal fluid proteins and show that the function of such proteins can be contingent upon female mating status. We also document effects of different size fractions on female mating receptivity and egg laying rates, which can serve as a basis for future efforts to identify the molecular identity of seminal fluid proteins and their function in this model species.

  20. Male seminal fluid substances affect sperm competition success and female reproductive behavior in a seed beetle.

    Science.gov (United States)

    Yamane, Takashi; Goenaga, Julieta; Rönn, Johanna Liljestrand; Arnqvist, Göran

    2015-01-01

    Male seminal fluid proteins are known to affect female reproductive behavior and physiology by reducing mating receptivity and by increasing egg production rates. Such substances are also though to increase the competitive fertilization success of males, but the empirical foundation for this tenet is restricted. Here, we examined the effects of injections of size-fractioned protein extracts from male reproductive organs on both male competitive fertilization success (i.e., P2 in double mating experiments) and female reproduction in the seed beetle Callosobruchus maculatus. We found that extracts of male seminal vesicles and ejaculatory ducts increased competitive fertilization success when males mated with females 1 day after the females' initial mating, while extracts from accessory glands and testes increased competitive fertilization success when males mated with females 2 days after the females' initial mating. Moreover, different size fractions of seminal fluid proteins had distinct and partly antagonistic effects on male competitive fertilization success. Collectively, our experiments show that several different seminal fluid proteins, deriving from different parts in the male reproductive tract and of different molecular weight, affect male competitive fertilization success in C. maculatus. Our results highlight the diverse effects of seminal fluid proteins and show that the function of such proteins can be contingent upon female mating status. We also document effects of different size fractions on female mating receptivity and egg laying rates, which can serve as a basis for future efforts to identify the molecular identity of seminal fluid proteins and their function in this model species.

  1. Higher Education and Class: Production or Reproduction?

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    Sotiris, Panagiotis

    2013-01-01

    This article deals with questions relating to the role of education and especially Higher Education in the reproduction of class division in society. Social classes and how they are formed and reproduced has always been one of the greatest challenges for Marxism and social theory in general. The questions regarding the role of education, and…

  2. The cost of dominance: suppressing subordinate reproduction affects the reproductive success of dominant female banded mongooses.

    Science.gov (United States)

    Bell, M B V; Nichols, H J; Gilchrist, J S; Cant, M A; Hodge, S J

    2012-02-07

    Social species show considerable variation in the extent to which dominant females suppress subordinate reproduction. Much of this variation may be influenced by the cost of active suppression to dominants, who may be selected to balance the need to maximize the resources available for their own offspring against the costs of interfering with subordinate reproduction. To date, the cost of reproductive suppression has received little attention, despite its potential to influence the outcome of conflict over the distribution of reproduction in social species. Here, we investigate possible costs of reproductive suppression in banded mongooses, where dominant females evict subordinates from their groups, thereby inducing subordinate abortion. We show that evicting subordinate females is associated with substantial costs to dominant females: pups born to females who evicted subordinates while pregnant were lighter than those born after undisturbed gestations; pups whose dependent period was disrupted by an eviction attained a lower weight at independence; and the proportion of a litter that survived to independence was reduced if there was an eviction during the dependent period. To our knowledge, this is the first empirical study indicating a possible cost to dominants in attempting to suppress subordinate breeding, and we argue that much of the variation in reproductive skew both within and between social species may be influenced by adaptive variation in the effort invested in suppression by dominants.

  3. Aspergillus asexual reproduction and sexual reproduction are differentially affected by transcriptional and translational mechanisms regulating stunted gene expression.

    Science.gov (United States)

    Wu, J; Miller, B L

    1997-10-01

    The Stunted protein (StuAp) is a member of a family of transcription factors that regulate fungal development and cell cycle progression. Regulated stuA gene expression is required for correct cell pattern formation during asexual reproduction (conidiation) and for initiation of the sexual reproductive cycle in Aspergillus nidulans. Transcriptional initiation from two different promoters yields overlapping mRNAs (stuA alpha and stuAbeta) that upon translation yield the same protein. Here we show that multiple regulatory mechanisms interact to control (i) developmental competence-dependent expression of both transcripts and (ii) induction-dependent expression of stuA alpha, but not stuAbeta, by the conidiation-specific Bristle (BrlAp) transcriptional activator. Quantitative levels of both mRNAs are further modulated by (i) an activator(s) located at a far-upstream upstream activation sequence, (ii) feedback regulation by StuAp, and (iii) positive translational regulation that requires the peptide product of a micro-open reading frame unique to the stuA alpha mRNA 5' untranslated region. Gradients in stuA alpha expression were most important for correct cell and tissue type development. Threshold requirements were as follows: metula-phialide differentiation < ascosporogenesis < cleistothecial shell-Hülle cell differentiation. Altered stuA expression affected conidiophore morphology and conidial yields quantitatively but did not alter the temporal development of cell types or conidiophore density. By contrast, the sexual cycle showed both temporal delay and quantitative reduction in the number of cleistothecial initials but normal morphogenesis of tissue types.

  4. PRODUCTIVITY, REPEATABILITY OF PRODUCTIVE AND REPRODUCTIVE TRAITS OF LOCAL PIGEON

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    S. Darwati

    2014-10-01

    Full Text Available The purpose of this study was to know productivity and repeatability of local pigeon. Data werecollected from 124 birds that reared under intensive management. The results showed that artificial pairwas 100% and polygamy was 16% (n=62 pair of pigeon. The ration of local pigeon consisting of 50%corn+50% of commercial feed for starter broiler chicken can be applied in field. The average of eggproduction was 1.8 eggs/pair/period, egg weight was 17.7 g, fertility was 96.6%, hatching rate was 77%,embryo mortality rate was 23%, interval period from laying to hatching and suckling was 51 days, 31.4days with hatching, and 17.6 days without hatching and suckling. The day old pigeon weight ranged10.9-16.2 g. Repeatability value of productive traits was high, in which egg weight was 0.64 and day oldpigeon weight was 0.737. Repeatability of reproductive traits was low, that was fertility and hatchabilitywas 0.12 and 0.048, respectively. The squab weight increased from week 0 to 4, then decreased in theweek 5. The growth rate was highest at the week 1, then decreased from the week 2 to 5 with thenegative growth rate occur at the 5th week. The squab growth rate followed a quadratic pattern. It wasconcluded that slaughter squab selection could be done at 4th week old.

  5. Heritability of rectal temperature and genetic correlations with production and reproduction traits in dairy cattle

    Science.gov (United States)

    Heat stress affects production and reproduction in dairy cattle. Genetic selection for body temperature might help to decrease the effects of heat stress on those traits. Objectives of the current study were a) to estimate genetic parameters of rectal temperature in dairy cows under heat stress cond...

  6. Pea (Pisum sativum) Seed Production as an Assay for Reproductive Effects Due to Herbicides.

    Science.gov (United States)

    Even though herbicide drift can affect plant reproduction, current plant testing protocols emphasize effects on vegetative growth. In this study, we determined whether a short–growing season plant can indicate potential effects of herbicides on seed production. Pea (Pisum sativum...

  7. Affective Productions of Mathematical Experience

    Science.gov (United States)

    Walshaw, Margaret; Brown, Tony

    2012-01-01

    In underscoring the affective elements of mathematics experience, we work with contemporary readings of the work of Spinoza on the politics of affect, to understand what is included in the cognitive repertoire of the Subject. We draw on those resources to tell a pedagogical tale about the relation between cognition and affect in settings of…

  8. Selection for production and reproduction traits in pigs.

    NARCIS (Netherlands)

    Vries, de A.G.

    1989-01-01

    IntroductionReproduction traits are important for piglet production, whereas production traits are important for fattening. Pig breeding organizations improve both groups of traits by selection in nucleus populations. Optimization of selection in these nucleus populations is important, becaus

  9. A survey of Canadian mechanical pulp and paper mill effluents: insights concerning the potential to affect fish reproduction.

    Science.gov (United States)

    Kovacs, Tibor G; Martel, Pierre H; O'Connor, Brian I; Hewitt, L Mark; Parrott, Joanne L; McMaster, Mark E; MacLatchy, Deborah L; Van Der Kraak, Glen J; Van Den Heuvel, Michael R

    2013-01-01

    Building on breakthroughs recently made at kraft mills, a survey of mechanical pulp and paper mill effluents was undertaken to gain insights concerning potential effects on fish reproduction. Effluents from seven Canadian mills were characterized chemically for conventional parameters such as biochemical oxygen demand (BOD) and total suspended solids (TSS). Each sample was further subjected to solvent extraction followed by gas chromatographic separation for the determination of resin/fatty acids and for the estimation of a gas chromatography (GC) profile index. Each mill effluent was assessed for the potential to affect fish reproduction in the laboratory using a five day adult fathead minnow (Pimephales promelas) egg production bioassay with exposures to 100% effluent. The seven effluents were found to have substantial variation both in terms of chemical characterization and effects on fish reproduction. Temporal variations were also noted in effluent quality at mills sampled on different occasions. Similar to what has been observed for kraft mills, a general trend of greater reductions in egg production caused by effluents with greater BOD concentrations and GC profile indices was noted. Effluents with BOD > 25 mg/L and GC Profile indices >5.0 caused a complete cessation of egg production. At the same time, about half of the total effluents sampled had BOD reproductive effects caused by such effluents is presently unclear. The effluent quality parameters considered in this study may require further refinement to address their utility in predicting the adverse reproductive effects induced by effluents from mechanical pulp and paper mills.

  10. Fathead minnow (Pimephales promelas) reproduction is impaired in aged oil sands process-affected waters.

    Science.gov (United States)

    Kavanagh, Richard J; Frank, Richard A; Oakes, Ken D; Servos, Mark R; Young, Rozlyn F; Fedorak, Phillip M; MacKinnon, Mike D; Solomon, Keith R; Dixon, D George; Van Der Kraak, Glen

    2011-01-17

    Large volumes of fluid tailings are generated during the extraction of bitumen from oil sands. As part of their reclamation plan, oil sands operators in Alberta propose to transfer these fluid tailings to end pit lakes and, over time, these are expected to develop lake habitats with productive capabilities comparable to natural lakes in the region. This study evaluates the potential impact of various oil sands process-affected waters (OSPW) on the reproduction of adult fathead minnow (Pimephales promelas) under laboratory conditions. Two separate assays with aged OPSW (>15 years) from the experimental ponds at Syncrude Canada Ltd. showed that water containing high concentrations of naphthenic acids (NAs; >25 mg/l) and elevated conductivity (>2000 μS/cm) completely inhibited spawning of fathead minnows and reduced male secondary sexual characteristics. Measurement of plasma sex steroid levels showed that male fathead minnows had lower concentrations of testosterone and 11-ketotestosterone whereas females had lower concentrations of 17β-estradiol. In a third assay, fathead minnows were first acclimated to the higher salinity conditions typical of OSPW for several weeks and then exposed to aged OSPW from Suncor Energy Inc. (NAs ∼40 mg/l and conductivity ∼2000 μS/cm). Spawning was significantly reduced in fathead minnows held in this effluent and male fathead minnows had lower concentrations of testosterone and 11-ketotestosterone. Collectively, these studies demonstrate that aged OSPW has the potential to negatively affect the reproductive physiology of fathead minnows and suggest that aquatic habitats with high NAs concentrations (>25 mg/l) and conductivities (>2000 μS/cm) would not be conducive for successful fish reproduction.

  11. Oyster reproduction is affected by exposure to polystyrene microplastics

    Science.gov (United States)

    Sussarellu, Rossana; Suquet, Marc; Thomas, Yoann; Lambert, Christophe; Fabioux, Caroline; Pernet, Marie Eve Julie; Le Goïc, Nelly; Quillien, Virgile; Mingant, Christian; Epelboin, Yanouk; Corporeau, Charlotte; Guyomarch, Julien; Robbens, Johan; Paul-Pont, Ika; Soudant, Philippe; Huvet, Arnaud

    2016-01-01

    Plastics are persistent synthetic polymers that accumulate as waste in the marine environment. Microplastic (MP) particles are derived from the breakdown of larger debris or can enter the environment as microscopic fragments. Because filter-feeder organisms ingest MP while feeding, they are likely to be impacted by MP pollution. To assess the impact of polystyrene microspheres (micro-PS) on the physiology of the Pacific oyster, adult oysters were experimentally exposed to virgin micro-PS (2 and 6 µm in diameter; 0.023 mg·L−1) for 2 mo during a reproductive cycle. Effects were investigated on ecophysiological parameters; cellular, transcriptomic, and proteomic responses; fecundity; and offspring development. Oysters preferentially ingested the 6-µm micro-PS over the 2-µm-diameter particles. Consumption of microalgae and absorption efficiency were significantly higher in exposed oysters, suggesting compensatory and physical effects on both digestive parameters. After 2 mo, exposed oysters had significant decreases in oocyte number (−38%), diameter (−5%), and sperm velocity (−23%). The D-larval yield and larval development of offspring derived from exposed parents decreased by 41% and 18%, respectively, compared with control offspring. Dynamic energy budget modeling, supported by transcriptomic profiles, suggested a significant shift of energy allocation from reproduction to structural growth, and elevated maintenance costs in exposed oysters, which is thought to be caused by interference with energy uptake. Molecular signatures of endocrine disruption were also revealed, but no endocrine disruptors were found in the biological samples. This study provides evidence that micro-PS cause feeding modifications and reproductive disruption in oysters, with significant impacts on offspring. PMID:26831072

  12. Oyster reproduction is affected by exposure to polystyrene microplastics.

    Science.gov (United States)

    Sussarellu, Rossana; Suquet, Marc; Thomas, Yoann; Lambert, Christophe; Fabioux, Caroline; Pernet, Marie Eve Julie; Le Goïc, Nelly; Quillien, Virgile; Mingant, Christian; Epelboin, Yanouk; Corporeau, Charlotte; Guyomarch, Julien; Robbens, Johan; Paul-Pont, Ika; Soudant, Philippe; Huvet, Arnaud

    2016-03-01

    Plastics are persistent synthetic polymers that accumulate as waste in the marine environment. Microplastic (MP) particles are derived from the breakdown of larger debris or can enter the environment as microscopic fragments. Because filter-feeder organisms ingest MP while feeding, they are likely to be impacted by MP pollution. To assess the impact of polystyrene microspheres (micro-PS) on the physiology of the Pacific oyster, adult oysters were experimentally exposed to virgin micro-PS (2 and 6 µm in diameter; 0.023 mg·L(-1)) for 2 mo during a reproductive cycle. Effects were investigated on ecophysiological parameters; cellular, transcriptomic, and proteomic responses; fecundity; and offspring development. Oysters preferentially ingested the 6-µm micro-PS over the 2-µm-diameter particles. Consumption of microalgae and absorption efficiency were significantly higher in exposed oysters, suggesting compensatory and physical effects on both digestive parameters. After 2 mo, exposed oysters had significant decreases in oocyte number (-38%), diameter (-5%), and sperm velocity (-23%). The D-larval yield and larval development of offspring derived from exposed parents decreased by 41% and 18%, respectively, compared with control offspring. Dynamic energy budget modeling, supported by transcriptomic profiles, suggested a significant shift of energy allocation from reproduction to structural growth, and elevated maintenance costs in exposed oysters, which is thought to be caused by interference with energy uptake. Molecular signatures of endocrine disruption were also revealed, but no endocrine disruptors were found in the biological samples. This study provides evidence that micro-PS cause feeding modifications and reproductive disruption in oysters, with significant impacts on offspring.

  13. Prey selectivity affects reproductive success of a corallivorous reef fish.

    Science.gov (United States)

    Brooker, Rohan M; Jones, Geoffrey P; Munday, Philip L

    2013-06-01

    Most animals consume a narrower range of food resources than is potentially available in the environment, but the underlying basis for these preferences is often poorly understood. Foraging theory predicts that prey selection should represent a trade-off between prey preferences based on nutritional value and prey availability. That is, species should consume preferred prey when available, but select less preferred prey when preferred prey is rare. We employed both field observation and laboratory experiments to examine the relationship between prey selection and preferences in the obligate coral-feeding filefish, Oxymonacanthus longirostris. To determine the drivers of prey selection, we experimentally established prey preferences in choice arenas and tested the consequences of prey preferences for key fitness-related parameters. Field studies showed that individuals fed almost exclusively on live corals from the genus Acropora. While diet was dominated by the most abundant species, Acropora nobilis, fish appeared to preferentially select rarer acroporids, such as A. millepora and A. hyacinthus. Prey choice experiments confirmed strong preferences for these corals, suggesting that field consumption is constrained by availability. In a longer-term feeding experiment, reproductive pairs fed on non-preferred corals exhibited dramatic reductions to body weight, and in hepatic and gonad condition, compared with those fed preferred corals. The majority of pairs fed preferred corals spawned frequently, while no spawning was observed for any pairs fed a non-preferred species of coral. These experiments suggest that fish distinguish between available corals based on their intrinsic value as prey, that reproductive success is dependent on the presence of particular coral species, and that differential loss of preferred corals could have serious consequences for the population success of these dietary specialists.

  14. Several synthetic progestins with different potencies adversely affect reproduction of fish.

    Science.gov (United States)

    Runnalls, Tamsin J; Beresford, Nicola; Losty, Erin; Scott, Alexander P; Sumpter, John P

    2013-02-19

    Synthetic progestins are widely used as a component in both contraceptives and in hormone replacement therapy (HRT), both on their own and in combination with EE2. Their presence in the environment is now established in wastewater effluent and river water and this has led to concerns regarding their potential effects on aquatic organisms living in these waters. We carried out in vivo experiments to determine the potencies of four different synthetic progestins on the reproductive capabilities of the fathead minnow (Pimephales promelas). We then performed a series of in vitro assays to try and determine the reason for the effects seen in the in vivo experiments. In the first experiment, fathead minnow exposed to a single concentration of 100 ng/L of either Levonorgestrel or Gestodene stopped spawning almost completely. The same nominal concentration of Desogestrel and Drospirenone did not affect reproduction (21 d NOECs of 100 ng/L). The second experiment investigated two progestins of different potency: Gestodene at 1, 10, and 100 ng/L and Desogestrel at 100 ng/L, 1 μg/L, and 10 μg/L. Gestodene concentrations as low as 1 ng/L had significant effects on reproduction over 21 d, whereas concentrations of Desogestrel at or above 1 μg/L were required to significantly reduce egg production. The synthetic progestins also masculinized the female fish in a concentration-dependent manner. Results from yeast-based in vitro assays demonstrated that the progestins are all strongly androgenic, thereby explaining the masculinization effects. The results strongly suggest that synthetic progestins merit serious consideration as environmental pollutants.

  15. Factors affecting reproductive success in three entomophilous orchid species in Hungary.

    Science.gov (United States)

    Vojtkó, Anna E; Sonkoly, Judit; Lukács, Balázs András; Molnár V, Attila

    2015-06-01

    The reproductive success of orchids is traditionally estimated by determining the fruit-set of individuals. Here, we investigated both the fruit and the seed production of three orchid species and the factors that may affect individual fruit-set, like pollination strategy, individual traits or the annual amount of precipitation. The species [Dactylorhiza sambucina (L.) Soó, Dactylorhiza majalis (Rchb.) P. F. Hunt & Summerhayes and Platanthera bifolia (L.) L. C. M. Richard] were studied in three consecutive years (2010-2012) in the Bükk Mountains, Hungary. All three species were proved to be non-autogamous by a bagging experiment. Data analyses showed significant differences between seed numbers but not between fruit-sets of species. There was no statistical difference in individual reproductive success between wet and dry years, however, the effect of the annual amount of precipitation is significant on the population level. Comparison of published fruit-set data revealed accordance with our results in P. bifolia, but not in D. sambucina and D. majalis. We assume that the surprisingly high fruit-set values of the two Dactylorhiza species may be due to the fact that the pollination crisis reported from Western European countries is not an actual problem in the Bükk Mountains, Hungary.

  16. Endocannabinoids affect the reproductive functions in teleosts and amphibians.

    Science.gov (United States)

    Cottone, E; Guastalla, A; Mackie, K; Franzoni, M F

    2008-04-16

    Following the discovery in the brain of the bonyfish Fugu rubripes of two genes encoding for type 1 cannabinoid receptors (CB1A and CB1B), investigations on the phylogeny of these receptors have indicated that the cannabinergic system is highly conserved. Among the multiple functions modulated by cannabinoids/endocannabinoids through the CB1 receptors one of the more investigated is the mammalian reproduction. Therefore, since studies performed in animal models other than mammals might provide further insight into the biology of these signalling molecules, the major aim of the present paper was to review the comparative data pointing toward the endocannabinoid involvement in the reproductive control of non-mammalian vertebrates, namely bonyfish and amphibians. The expression and distribution of CB1 receptors were investigated in the CNS and gonads of two teleosts, Pelvicachromis pulcher and Carassius auratus as well as in the anuran amphibians Xenopus laevis and Rana esculenta. In general the large diffusion of neurons targeted by cannabinoids in both fish and amphibian forebrain indicate endocannabinoids as pivotal local messengers in several neural circuits involved in either sensory integrative activities, like the olfactory processes (in amphibians) and food response (in bonyfish), or neuroendocrine machinery (in both). By using immunohistochemistry for CB1 and GnRH-I, the codistribution of the two signalling molecules was found in the fish basal telencephalon and preoptic area, which are key centers for gonadotropic regulation in all vertebrates. A similar topographical codistribution was observed also in the septum of the telencephalon in Rana esculenta and Xenopus laevis. Interestingly, the double standard immunofluorescence on the same brain section, aided with a laser confocal microscope, showed that in anurans a subset of GnRH-I neurons exhibited also the CB1 immunostaining. The fact that CB1-LI-IR was found indeed in the FSH gonadotrophs of the Xenopus

  17. Implant Size Availability Affects Reproduction of Distal Femoral Anatomy.

    Science.gov (United States)

    Morris, William Z; Gebhart, Jeremy J; Goldberg, Victor M; Wera, Glenn D

    2016-07-01

    A total knee arthroplasty system offers more distal femoral implant anterior-posterior (AP) sizes than its predecessor. The purpose of this study is to investigate the impact of increased size availability on an implant system's ability to reproduce the AP dimension of the native distal femur. We measured 200 cadaveric femora with the AP-sizing guides of Zimmer (Warsaw, IN) NexGen (8 sizes) and Zimmer Persona (12 sizes) total knee arthroplasty systems. We defined "size deviation" as the difference in the AP dimension between the anatomic size of the native femur and the closest implant size. We defined satisfactory reproduction of distal femoral dimensions as Persona (p Persona. Only 1/200 specimens (0.5%) was a poor fit by Persona, but a satisfactory fit by NexGen (p < 0.001). The novel knee system with 12 versus 8 sizes reproduces the AP dimension of the native distal femur more closely than its predecessor. Further study is needed to determine the clinical impact of these differences.

  18. How Employee Turnover Affects Productivity

    DEFF Research Database (Denmark)

    Eriksen, Bo

    Research on employee turnover suggests that turnover results in negative organization-level outcomes. This paper provides a firm-level analysis of the impact of the in- and outflows of human resources on productivity and how the presence of organizational slack resources moderates the effects...

  19. How Employee Turnover Affects Productivity

    DEFF Research Database (Denmark)

    Eriksen, Bo

    Research on employee turnover suggests that turnover results in negative organization-level outcomes. This paper provides a firm-level analysis of the impact of the in- and outflows of human resources on productivity and how the presence of organizational slack resources moderates the effects...... of employee turnover. Drawing on a unique longitudinal dataset of 2,926 Danish manufacturing firms that combine individual-level data with firm-level data, the paper shows that job turnover has a substantial negative effect on total productivity but that the firm’s size, its capital intensity, and its age...... moderate this effect so that the negative consequences of employee turnover are less severe for larger, older and capital intensive firms. These moderating variables indicate the presence of slack resources in the firm, and thus that the accumulation of slack reduces the efficiency losses from employee...

  20. 新疆产药用植物黑果枸杞有性生殖产出差异的繁殖生态学研究%Reproductive Ecology Research on Populations of a Medicinal Plant (Lycium ruthenicum Murr.) from Xinjiang Reveals Factors Affecting Fruit Production

    Institute of Scientific and Technical Information of China (English)

    阿力同·其米克; 金晓芳; 叶忠铭; 王青锋; 陈进明; 杨春锋

    2014-01-01

    由于黑果枸杞(Lycium ruthenicum)果实具有重要的保健价值,近年来倍受关注,但其野生资源正遭受着前所未有的采伐压力.目前关于影响黑果枸杞果实产量的有性生殖过程研究较少,本实验对新疆库尔勒地区6个不同生境的野生黑果枸杞居群开展了繁殖生态学观察,并对各居群的访花频率、访花昆虫种类、柱头花粉落置、胚珠数、座果率、结实率和单株果实产量等指标进行了比较分析.结果表明,访花频率与结实率、座果率具有显著正相关关系,说明传粉者是限制黑果枸杞有性生殖的关键因素之一;传粉者种类越丰富的居群其有性生殖产出越高,揭示了传粉昆虫的多样性和丰富度是影响黑果枸杞繁殖成功的重要限制因子.此外,和静县华侨农场和焉耆县霍拉山河沟的黑果枸杞居群生殖产出明显与其传粉成功率相偏离,进一步分析表明,风沙侵蚀和人为采伐对植株的破坏是影响这两个居群果实产量的重要原因.本研究结果对于野生黑果枸杞居群的保护及其人工规模栽培提供了重要的参考依据.%Recently,Lycium ruthenicum attracted substantial attention due to the high value of its fruits to human health.As a result,the wild resources are at high risk of loss and detailed studies on the sexual reproductive ecology of this important medicinal plant should be conducted for better conservation.We investigated factors affecting reproductive output of the plant using six wild populations from Xinjiang.We compared differences in pollination frequency,pollinator composition,stigmatic pollen load,and ovule production among the populations,as well as correlations with reproductive output namely,fruit set,seed set and fruit production per plant.Results indicated that pollination frequency was significantly correlated with fruit set and seed set,and populations with high diversity in pollinator types had higher reproductive output

  1. Product versus additive threshold models for analysis of reproduction outcomes in animal genetics.

    Science.gov (United States)

    David, I; Bodin, L; Gianola, D; Legarra, A; Manfredi, E; Robert-Granié, C

    2009-08-01

    The phenotypic observation of some reproduction traits (e.g., insemination success, interval from lambing to insemination) is the result of environmental and genetic factors acting on 2 individuals: the male and female involved in a mating couple. In animal genetics, the main approach (called additive model) proposed for studying such traits assumes that the phenotype is linked to a purely additive combination, either on the observed scale for continuous traits or on some underlying scale for discrete traits, of environmental and genetic effects affecting the 2 individuals. Statistical models proposed for studying human fecundability generally consider reproduction outcomes as the product of hypothetical unobservable variables. Taking inspiration from these works, we propose a model (product threshold model) for studying a binary reproduction trait that supposes that the observed phenotype is the product of 2 unobserved phenotypes, 1 for each individual. We developed a Gibbs sampling algorithm for fitting a Bayesian product threshold model including additive genetic effects and showed by simulation that it is feasible and that it provides good estimates of the parameters. We showed that fitting an additive threshold model to data that are simulated under a product threshold model provides biased estimates, especially for individuals with high breeding values. A main advantage of the product threshold model is that, in contrast to the additive model, it provides distinct estimates of fixed effects affecting each of the 2 unobserved phenotypes.

  2. Productive and reproductive performance of crossbred Holstein x Gir cows

    Directory of Open Access Journals (Sweden)

    Alexandre Balancin Júnior

    2014-10-01

    Full Text Available This study aimed to evaluate the productive and reproductive performance of Holtein-Frisian x Gir crossbred cows. The traits evaluated were: milk production up to 305 days (P305, total milk production (PTL, production per day of calving interval (PIP, age at first calving (IPP, calving interval (IDP, service period (PS and gestation length (PG, which were analyzed using mixed linear models. The Incomplete gamma function was used to estimate the parameters of lactation curves for each genetic group. The animals of genetic groups (GG 3/4H, 7/8H and pure by crosses (PC achieved higher performances for productive traits, and animals from the GG 1/2H achieved better performance for reproductive traits. The Holstein-Frisian PC animals presented the lactation curves with higher production levels and higher productions at the peak of the lactation but with less persistence. The 1/2H animals had slightly lower persistence in comparison to genetic groups 3/4H and 7/8H. Milk production in function of gestation length varied according to each group and Holstein-Frisian (PC presented greater decrease in milk yield after conception.

  3. Factors Affecting the Productivity of Government Workers

    Directory of Open Access Journals (Sweden)

    Jerry P. Haenisch

    2012-01-01

    Full Text Available While there have been a variety of studies concerning government worker motivation and productivity, few, if any, studies have focused specifically on state government workers’ perceptions about what factors affect their productivity. With more than 5 million workers employed by state governments in the United States, any improvement in state workplace productivity could have significant financial and service impact for society. In this study, state government workers identified those factors perceived as most affecting their workplace productivity. Data were collected through a survey offered to state government workers in the state of Wyoming. Factor analysis was used to derive key productivity factors from survey responses. The results indicate that state government workers appreciate having freedom and autonomy, like their jobs and the sense of achievement, and welcome teamwork, but feel limited by poor supervision and management, poor communications, and insufficient budgets and staffing. To improve productivity, the workers would eliminate bureaucracy, supervise better, and improve communication.

  4. Does Labour Diversity affect Firm Productivity?

    DEFF Research Database (Denmark)

    Parrotta, Pierpaolo; Pozzoli, Dario; Pytlikova, Mariola

    that labor diversity in education significantly enhances a firm's value added. Conversely, diversity in ethnicity and demographics induces negative effects on firm productivity. Hence, the negative effects, coming from communication and integration costs connected to a more culturally and demographically......Using a matched employer-employee dataset, we analyze how workforce diversity in cultural background, education and demographic characteristics affects productivity of firms in Denmark. Implementing a structural estimation of the firms' production function (Ackerberg et al., 2006) we find...

  5. Cold storage affects mortality, body mass, lifespan, reproduction and flight capacity of Praon volucre (Hymenoptera: Braconidae)

    NARCIS (Netherlands)

    Lins, J.C.; Bueno, V.H.P.; Sidney, L.A.; Silva, D.B.; Sampaio, M.V.; Pereira, J.M.; Nomelini, Q.S.S.; Lenteren, van J.C.

    2013-01-01

    The possibility of storing natural enemies at low temperatures is important for the mass production of biological control agents. We evaluated the effect of different periods of cold storage on immature mortality, mummy body mass, lifespan, reproduction and flight capacity of the parasitoid Praon vo

  6. Both morph- and species-dependent asymmetries affect reproductive barriers between heterostylous species.

    Science.gov (United States)

    Keller, Barbara; de Vos, Jurriaan M; Schmidt-Lebuhn, Alexander N; Thomson, James D; Conti, Elena

    2016-09-01

    The interaction between floral traits and reproductive isolation is crucial to explaining the extraordinary diversity of angiosperms. Heterostyly, a complex floral polymorphism that optimizes outcrossing, evolved repeatedly and has been shown to accelerate diversification in primroses, yet its potential influence on isolating mechanisms remains unexplored. Furthermore, the relative contribution of pre- versus postmating barriers to reproductive isolation is still debated. No experimental study has yet evaluated the possible effects of heterostyly on pre- and postmating reproductive mechanisms. We quantify multiple reproductive barriers between the heterostylous Primula elatior (oxlip) and P. vulgaris (primrose), which readily hybridize when co-occurring, and test whether traits of heterostyly contribute to reproductive barriers in unique ways. We find that premating isolation is key for both species, while postmating isolation is considerable only for P. vulgaris; ecogeographic isolation is crucial for both species, while phenological, seed developmental, and hybrid sterility barriers are also important in P. vulgaris, implicating sympatrically higher gene flow into P. elatior. We document for the first time that, in addition to the aforementioned species-dependent asymmetries, morph-dependent asymmetries affect reproductive barriers between heterostylous species. Indeed, the interspecific decrease of reciprocity between high sexual organs of complementary floral morphs limits interspecific pollen transfer from anthers of short-styled flowers to stigmas of long-styled flowers, while higher reciprocity between low sexual organs favors introgression over isolation from anthers of long-styled flowers to stigmas of short-styled flowers. Finally, intramorph incompatibility persists across species boundaries, but is weakened in long-styled flowers of P. elatior, opening a possible backdoor to gene flow through intramorph pollen transfer between species. Therefore

  7. Inbreeding on productive and reproductive traits of dairy Gyr cattle

    Directory of Open Access Journals (Sweden)

    João Cruz Reis Filho

    2015-05-01

    Full Text Available The objective of this study was to estimate genetic parameters and to evaluate the effects of inbreeding on productive and reproductive traits of dairy Gyr cattle. Single-trait animal models were used to estimate genetic parameters and solutions for inbreeding coefficients for milk (milk 305-d, fat (fat 305-d, protein (protein 305-d, lactose (lactose 305-d, and total solids (TS 305-d yield up to 305 days of lactation, days in milk (DIM, age at first calving (AFC and calving intervals (CI. The mean inbreeding coefficient was 2.82%. The models with linear and quadratic effects of inbreeding coefficients fitted the data better than the models without or with only linear effect of inbreeding coefficient for all traits. The increase in inbreeding coefficient caused several losses in productive and reproductive traits of dairy Gyr cattle. Estimates of heritability for milk 305-d, fat 305-d, protein 305-d, lactose 305-d, TS 305-d, DIM, AFC, and CI were 0.28, 0.27, 0.22, 0.21, 0.22, 0.17, 0.20, and 0.10, respectively. It is possible to achieve genetic progress in productive traits (especially in milk 305-d and fat 305-d and age at first calving in dairy Gyr cattle through selection.

  8. The marketing implications of affective product design.

    Science.gov (United States)

    Seva, Rosemary R; Duh, Henry Been-Lirn; Helander, Martin G

    2007-11-01

    Emotions are compelling human experiences and product designers can take advantage of this by conceptualizing emotion-engendering products that sell well in the market. This study hypothesized that product attributes influence users' emotions and that the relationship is moderated by the adherence of these product attributes to purchase criteria. It was further hypothesized that the emotional experience of the user influences purchase intention. A laboratory study was conducted to validate the hypotheses using mobile phones as test products. Sixty-two participants were asked to assess eight phones from a display of 10 phones and indicate their emotional experiences after assessment. Results suggest that some product attributes can cause intense emotional experience. The attributes relate to the phone's dimensions and the relationship between these dimensions. The study validated the notion of integrating affect in designing products that convey users' personalities.

  9. Adult carbohydrate feeding affects reproduction of Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)

    Energy Technology Data Exchange (ETDEWEB)

    Jordao, Alexandre L., E-mail: aljordao@hotmail.co [Instituto de Pesquisas Cientificas e Tecnologicas do Estado do Amapa (IEPA), Macapa, AP (Brazil); Nakano, Octavio, E-mail: onakano@esalq.usp.b [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil). Dept. de Entomologia e Acarologia; Janeiro, Vanderly, E-mail: vjaneiro@hotmail.co [Universidade Estadual de Maringa (UEM), PR (Brazil). Dept. de Estatistica

    2010-06-15

    Reproduction of most insects depend on nutrients accumulated during the larval stage, but many lepidopteran species will also depend on nutrients obtained at the adult stage. Feeding at the adult stage allows the intake of carbohydrate and amino acid rich solutions, which may have an effect on the species reproduction and population growth. The objectives of the current study were to characterize the effects of sugar consumption by adults of the potato tuber moth, Phthorimaea operculella (Zeller), on its reproduction. To initially test the food intake by adults, a 10% honey solution or water (control), both containing a liquid dye were offered to adult insects 24 h after emergence, and the presence of the dye was observed by analysis of their digestive system. The effects of adult feeding on a 10% honey solution on the reproductive performance of P. operculella were evaluated by assessing the oviposition rate, fecundity and fertility. Adult feeding was proved by the presence of the dye within the digestive system of adults of P. operculella. Although the oviposition rate and fertility were not affected by adult feeding, female fecundity was higher in honey-fed females as compared to the water-fed females. (author)

  10. Dystocia in Friesian cows and its effects on postpartum reproductive performance and milk production.

    Science.gov (United States)

    Gaafar, H M A; Shamiah, Sh M; El-Hamd, M A Abu; Shitta, A A; El-Din, M A Tag

    2011-01-01

    A total of 1,243 records for 585 dairy Friesian cows from 1997-2004 were used to study the factors affecting dystocia and its effects on reproductive performance and milk production. The overall incidence of dystocia was 6.9%. The percentage of dystocia decreased with increasing live body weight, age, and parity of cows (P dystocia was detected in winter season, but the least percentage was in summer season (P dystocia was significantly (P dystocia was significantly (P dystocia had adverse effects on reproductive performance and milk yield. The service interval, service period, days open, and calving interval were significantly (P dystocia compared to normal cows. The conception rate was lower (P dystocia compared to normal cows (60.5% vs. 73.0% and 3.4 vs. 2.7, respectively). Average daily milk yield was lower (P dystocia compared to normal cows.

  11. Caterpillar biomass depends on temperature and precipitation, but does not affect bird reproduction

    Science.gov (United States)

    Schöll, Eva Maria; Ohm, Judith; Hoffmann, Konstantin Frank; Hille, Sabine Marlene

    2016-07-01

    Complex changes in phenological events appear as temperatures are increasing: In deciduous forests bud burst, hatching of herbivorous caterpillars, egg laying and nestling time of birds when feeding chicks on caterpillars, may differentially shift into early season and alter synchronization. If timing of bird reproduction has to match with short periods of food availability, phenological mismatch could negatively affect reproductive success. Using a unique empirical approach along an altitudinal temperature gradient, we firstly asked whether besides temperature, also precipitation and leaf phenology interplay and affect caterpillar biomass, since impacts of rainfall on caterpillars have been largely neglected so far. Secondly, we asked whether abundance of caterpillars and thereby body mass of great tit nestlings, which are mainly fed with caterpillars, vary along the altitudinal temperature gradient. We demonstrated that next to temperature also precipitation and leaf phenology affected caterpillar biomass. In our beech forest, even along altitudes, caterpillars were available throughout the great tit breeding season but in highly variable amounts. Our findings revealed that although timing of leaf phenology and great tit breeding season were delayed with decreasing temperature, caterpillars occurred synchronously and were not delayed according to altitude. However, altitude negatively affected caterpillar biomass, but body mass of fledglings at high altitude sites was not affected by lower amounts of caterpillar biomass. This might be partially outweighed by larger territory sizes in great tits.

  12. Repeatability and genotypic correlations of reproductive and productive traits of crossbred beef cattle dams.

    Science.gov (United States)

    Silva, L N; Gasparino, E; Torres Júnior, R A A; Euclides Filho, K; Silva, L O C; Alencar, M M; Souza Júnior, M D; Battistelli, J V F; Silva, S C C

    2015-05-22

    Beef cattle production requires reproductive efficiency. However, measures of reproductive traits are not usually collected; consequently, correlated traits that could be used as indicators would be useful. We examined associations between measures of reproductive and productive efficiency that could be used as selection indicators. Data from 194 dams of the genetic groups Angus x Nelore, Caracu x Nelore, and Valdostana x Nelore collected over 4 years were used. The reproductive traits analyzed were days to heat (DH), calving interval (CI), days to calving (DC), and pregnancy rate (PR). The productive traits were dam weight (DW), body condition score (BCS), calf weight (CW), and weaning rate (WR). The effects on the model were: year, genetic group, reproductive status (RS), age, reproductive rest, and breed of bull (CW and WR). Multivariate analyses were performed, using the Bayesian approach via Gibbs sampling. We conclude that the reproductive measures are ineffective as selection indicators, whereas using dam weight may be a good alternative.

  13. Oral exposure of adult zebrafish (Danio rerio) to 2,4,6-tribromophenol affects reproduction

    DEFF Research Database (Denmark)

    Halden, Anna Norman; Nyholm, Jenny Rattfelt; Andersson, Patrik L;

    2010-01-01

    was not significantly affected, but yolk-sac oedema tended to increase in frequency in exposed groups with time. Our results show that dietary exposure to TBP, at concentrations found in marine organisms that are part of the natural diet of wild fish, can interfere with reproduction in zebrafish. We also observed low......The bromophenol 2,4,6-tribromophenol (TBP) is widely used as an industrial chemical, formed by degradation of tetrabromobisphenol-A, and it occurs naturally in marine organisms. Concentrations of TBP in fish have been related to intake via feed, but little is known about effects on fish health...... after oral exposure. In this study, we exposed adult male and female zebrafish (Danio rerio) to TBP via feed in nominal concentrations of 33, 330, and 3300 mu g/g feed (or control feed) for 6 weeks to assess the effects of TBP on reproductive output, gonad morphology, circulatory vitellogenin levels...

  14. You are what you eat: food limitation affects reproductive fitness in a sexually cannibalistic praying mantid.

    Directory of Open Access Journals (Sweden)

    Katherine L Barry

    Full Text Available Resource limitation during the juvenile stages frequently results in developmental delays and reduced size at maturity, and dietary restriction during adulthood can affect longevity and reproductive output. Variation in food intake can also result in alteration to the normal pattern of resource allocation among body parts or life-history stages. My primary aim in this study was to determine how varying juvenile and/or adult feeding regimes affect particular female and male traits in the sexually cannibalistic praying mantid Pseudomantis albofimbriata. Praying mantids are sit-and-wait predators whose resource intake can vary dramatically depending on environmental conditions within and across seasons, making them useful for studying the effects of feeding regime on various facets of reproductive fitness. In this study, there was a significant trend/difference in development and morphology for males and females as a result of juvenile feeding treatment, however, its effect on the fitness components measured for males was much greater than on those measured for females. Food-limited males were less likely to find a female during field enclosure experiments and smaller males were slower at finding a female in field-based experiments, providing some of the first empirical evidence of a large male size advantage for scrambling males. Only adult food limitation affected female fecundity, and the ability of a female to chemically attract males was also most notably affected by adult feeding regime (although juvenile food limitation did play a role. Furthermore, the significant difference/trend in all male traits and the lack of difference in male trait ratios between treatments suggests a proportional distribution of resources and, therefore, no trait conservation by food-limited males. This study provides evidence that males and females are under different selective pressures with respect to resource acquisition and is also one of very few to show an

  15. You are what you eat: food limitation affects reproductive fitness in a sexually cannibalistic praying mantid.

    Science.gov (United States)

    Barry, Katherine L

    2013-01-01

    Resource limitation during the juvenile stages frequently results in developmental delays and reduced size at maturity, and dietary restriction during adulthood can affect longevity and reproductive output. Variation in food intake can also result in alteration to the normal pattern of resource allocation among body parts or life-history stages. My primary aim in this study was to determine how varying juvenile and/or adult feeding regimes affect particular female and male traits in the sexually cannibalistic praying mantid Pseudomantis albofimbriata. Praying mantids are sit-and-wait predators whose resource intake can vary dramatically depending on environmental conditions within and across seasons, making them useful for studying the effects of feeding regime on various facets of reproductive fitness. In this study, there was a significant trend/difference in development and morphology for males and females as a result of juvenile feeding treatment, however, its effect on the fitness components measured for males was much greater than on those measured for females. Food-limited males were less likely to find a female during field enclosure experiments and smaller males were slower at finding a female in field-based experiments, providing some of the first empirical evidence of a large male size advantage for scrambling males. Only adult food limitation affected female fecundity, and the ability of a female to chemically attract males was also most notably affected by adult feeding regime (although juvenile food limitation did play a role). Furthermore, the significant difference/trend in all male traits and the lack of difference in male trait ratios between treatments suggests a proportional distribution of resources and, therefore, no trait conservation by food-limited males. This study provides evidence that males and females are under different selective pressures with respect to resource acquisition and is also one of very few to show an effect of juvenile

  16. Food and predators affect egg production in song sparrows.

    Science.gov (United States)

    Zanette, Liana; Clinchy, Michael; Smith, James N M

    2006-10-01

    Although the possibility that food and predators may interact in limiting avian populations has long been recognized, there have been few attempts to test this experimentally in the field. We conducted a manipulative food addition experiment on the demography of Song Sparrows (Melospiza melodia) across sites that varied in predator abundance, near Victoria, British Columbia, Canada, over three consecutive breeding seasons. We previously showed that food and predators had interactive effects on annual reproductive success (young fledged per female). Here, we report the effects on egg production. Our results show that food limits the total number of eggs laid over the breeding season ("total egg production") and that interactive food and predator effects, including food effects on nest predation, determine how those eggs are "parceled out" into different nests. Food addition alone significantly affected total egg production, and there was no significant interannual variability in this result. At the same time, both food and predators affected the two determinants of total egg production: "clutch number" (total number of clutches laid) and average clutch size. Both clutch number and size were affected by a food x predator x year interaction. Clutch number was lower at low-predator locations because there was less nest predation and thus less renesting. Food addition also significantly reduced nest predation, but there was significant interannual variation in this effect. This interannual variation was responsible for the food x predator x year interactions because the larger the effect of food on nest predation in a given year, the smaller was the effect of food on clutch number; and the smaller the effect of food on clutch number, the larger was the effect of food on clutch size. Potential predator and year effects on total egg production were thus cancelled out by an inverse relationship between clutch number and clutch size. We suggest that combined food and

  17. Factors affecting the reproductive success of American Oystercatchers Haematopus palliatus on the outer banks of North Carolina

    Science.gov (United States)

    Schulte, Shiloh A.; Simons, Theodore R.

    2015-01-01

    We used an information-theoretic approach to assess the factors affecting the reproductive success of American Oystercatchers Haematopus palliatus on the Outer Banks of North Carolina. We evaluated survival with respect to nesting island, year, time of season, brood age, distance to tide (m), presence of off-road vehicles and proximity of foraging habitat. The daily nest survival (mean 0.981, standard error [SE] 0.002) was affected by year and island, and declined over the nesting season. Mammals were responsible for 54% of identified nest failures. Daily brood survival (mean 0.981, SE 0.002) varied by island and increased non-linearly with age, with highest mortality in the seven days after hatching. Model results indicate direct access to foraging sites has a positive effect on brood survival, whereas presence of off-road vehicles has a negative effect. We studied chick behavior and survival using radio telemetry and direct observation and found that vehicles caused mortality and affected behavior and resource use by oystercatcher chicks. We identified the source of mortality for 37 radio-tagged chicks. Six (16%) were killed by vehicles, 21 (57%) by predators, and 10 (27%) by exposure and starvation. From 1995 to 2008, 25 additional oystercatcher chicks were found dead, 13 (52%) killed by vehicles. Chicks on beaches closed to vehicles used beach and intertidal zones more frequently than chicks on beaches open to vehicles. Chick predators included Great Horned Owls Bubo virginianus, Fish Crows Corvus ossifragus, cats Felis catus, mink Mustela vison, raccoons Procyon lotor, and ghost crabs Ocypode albicans. The factors affecting reproductive success differed between the incubation and chick-rearing stages.  Management actions that influence chick survival will have a larger effect on total productivity than actions affecting nest survival.

  18. Fishing top predators indirectly affects condition and reproduction in a reef-fish community.

    Science.gov (United States)

    Walsh, S M; Hamilton, S L; Ruttenberg, B I; Donovan, M K; Sandin, S A

    2012-03-01

    To examine the indirect effects of fishing on energy allocation in non-target prey species, condition and reproductive potential were measured for five representative species (two-spot red snapper Lutjanus bohar, arc-eye hawkfish Paracirrhites arcatus, blackbar devil Plectroglyphidodon dickii, bicolour chromis Chromis margaritifer and whitecheek surgeonfish Acanthurus nigricans) from three reef-fish communities with different levels of fishing and predator abundance in the northern Line Islands, central Pacific Ocean. Predator abundance differed by five to seven-fold among islands, and despite no clear differences in prey abundance, differences in prey condition and reproductive potential among islands were found. Body condition (mean body mass adjusted for length) was consistently lower at sites with higher predator abundance for three of the four prey species. Mean liver mass (adjusted for total body mass), an indicator of energy reserves, was also lower at sites with higher predator abundance for three of the prey species and the predator. Trends in reproductive potential were less clear. Mean gonad mass (adjusted for total body mass) was high where predator abundance was high for only one of the three species in which it was measured. Evidence of consistently low prey body condition and energy reserves in a diverse suite of species at reefs with high predator abundance suggests that fishing may indirectly affect non-target prey-fish populations through changes in predation and predation risk.

  19. Factors that affect the reproductive efficiency of the recipient within a bovine embryo transfer program

    Directory of Open Access Journals (Sweden)

    Arturo Duica A.

    2007-12-01

    Full Text Available The embryo transfer is a biotechnological technique that allows increasing the descendant of animals with high genetic value. The positive results, represented in pregnancy after the application of this technique, are affected by some factors that are inherent to the donor, the embryo, the technique, and the recipients which receive a strange embryo in the uterus allowing pregnancy. This review describes some factors affecting the reproductive efficiency of the recipients of bovine embryos within a program of embryo transfer. Its important to evaluate the parameters in this kind of recipients, as race, age, physiological status, health status, weight, reproductive tract integrity and management, and also too monitoring the ovarian structures while the estrus synchronization, and within previous and posterior stages in embryo transfer procedure. Therefore an optimum follicular development will be determinant to corpus luteum formation which generates enough serum progesterone concentrations to offer a right uterine environment allowing the optimum embryo development. Controlling the factors that affect the efficiency of the embryo transfer, it will obtain an increasing of positive results represented in pregnancies and births of individuals come from animals with high genetic value.

  20. Microcystin-LR impairs zebrafish reproduction by affecting oogenesis and endocrine system.

    Science.gov (United States)

    Zhao, Yanyan; Xie, Liqiang; Yan, Yunjun

    2015-02-01

    Previous studies have shown that microcystins (MCs) are able to exert negative effects on the reproductive system of fish. However, few data are actually available on the effects of MC-LR on the reproductive system of female fish. In the present study, female zebrafish were exposed to 2, 10, and 50 μg L(-1) of MC-LR for 21 d, and its effects on oogenesis, sex hormones, transcription of genes on the hypothalamic-pituitary-gonad (HPG) axis, and reproduction were investigated for the first time. It was observed that egg production significantly declined at ⩾ 10 μg L(-1) MC-LR. MC-LR exposure to zebrafish increased the concentrations of 17β-estradiol (E2) and vitellogenin (VTG) at 10 μg L(-1) level, whereas concentrations of E2, VTG and testosterone declined at 50 μg L(-1) MC-LR. The transcriptions of steroidogenic pathway gene (cyp19a, cyp19b, 17βhsd, cyp17 and hmgra) changed as well after the exposure and corresponded well with the alterations of hormone levels. A number of intra- and extra-ovarian factors, such as gnrh3, gnrhr1, fshβ, fshr, lhr, bmp15, mrpβ, ptgs2 and vtg1 which regulate oogenesis, were significantly changed with a different dose-related effect. Moreover, MC-LR exposure to female zebrafish resulted in decreased fertilization and hatching rates, and may suggest the possibility of trans-generational effects of MC-LR exposure. The results demonstrate that MC-LR could modulate endocrine function and oogenesis, eventually leading to disruption of reproductive performance in female zebrafish. These data suggest there is a risk for aquatic population living in MC polluted areas.

  1. Hormonal, biochemical, and hematological profiles in female camels (Camelus dromedarius) affected with reproductive disorders.

    Science.gov (United States)

    Ali, A; Tharwat, M; Al-Sobayil, F A

    2010-04-01

    The aim of this study was to assess the blood profiles in female camels affected with common reproductive disorders. Estradiol-17beta (E(2)), progesterone (P(4)), thyroxin (T(4)), zinc (Zn), copper (Cu), calcium (Ca), phosphorus (P), magnesium (Mg), cholesterol, glucose, triglycerides, total protein, albumin, globulin, hematocrite, and total and differential white blood cell counts (WBC) were determined in blood of female camels affected with endometritis (n=15), vaginal adhesions (n=15), and ovarian cysts (n=15). Normal cyclic animals were used as controls (n=15). Diagnosis of reproductive disorders was based on transrectal palpation, ultrasonographic examination, and exploration of the vagina. Increased WBC counts (P=0.03) and a tendency for neutrophelia (P=0.05) were noted in female camels with vaginal adhesions. These animals were also characterized by having higher concentration of serum P(4) (P=0.0001), T(4) (P=0.001) and total protein (P=0.007), in comparison with female camels with endometritis, ovarian cysts, or controls. Animals having ovarian cysts with thin walls and homogenous hypoechogenic contents had greater serum E(2) (P=0.001) and P(4) (P=0.0001) than those having ovarian cysts with thick walls and non-homogenous echogenic contents. Animals with endometritis, vaginal adhesions, and ovarian cysts revealed lower serum Zn concentration than that of control group (P=0.003). Other blood parameters did not differ significantly compared to controls. In conclusion, this is the first report characterizing blood constituents in female camels with various reproductive disorders. These profiles may be valuable in clarifying the etio-pathogenesis of these disorders.

  2. Factors Affecting Sugarcane Production in Pakistan

    Directory of Open Access Journals (Sweden)

    Adnan Nazir

    2013-05-01

    Full Text Available This study was undertaken to identify the factors affecting sugarcane production in Pakistan. Data were collected from 387 sugarcane growers from Sindh, Punjab and NWFP province. Data were collected during the period 2007-08. The study reveals that the costs of inputs of sugarcane i.e. urea, DAP, FYM, land preparation, seed and its application, weeding and cost of irrigation were the important factors which influenced on the returns of sugarcane growers. The effectiveness was examined by using the Cobb-Douglas production function; MVP and allocative efficiency were calculated. The coefficient of multiple determinations R2 was 0.9249, which indicated that 92% variation in the cost of inputs was explained by all explanatory variables and the adjusted R2 was 92%. The F-value was 666.94 and was highly significant at 5% level of significance, indicating that the regression model was well fitted. The high prices of inputs, low price of output, delay in payments and lack of scientific knowledge were the major problems in sugarcane production. In order to enhance the productivity of sugarcane in the country, government should solve the identified problems to increase the income of sugarcane growers.

  3. Selenium status affects selenoprotein expression, reproduction, and F₁ generation locomotor activity in zebrafish (Danio rerio).

    Science.gov (United States)

    Penglase, Sam; Hamre, Kristin; Rasinger, Josef D; Ellingsen, Staale

    2014-06-14

    Se is an essential trace element, and is incorporated into selenoproteins which play important roles in human health. Mammalian selenoprotein-coding genes are often present as paralogues in teleost fish, and it is unclear whether the expression patterns or functions of these fish paralogues reflect their mammalian orthologues. Using the model species zebrafish (Danio rerio; ZF), we aimed to assess how dietary Se affects key parameters in Se metabolism and utilisation including glutathione peroxidase (GPX) activity, the mRNA expression of key Se-dependent proteins (gpx1a, gpx1b, sepp1a and sepp1b), oxidative status, reproductive success and F1 generation locomotor activity. From 27 d until 254 d post-fertilisation, ZF were fed diets with graded levels of Se ranging from deficient ( levels were lowest when dietary Se levels (0·3 mg/kg) resulted in the maximum growth of ZF, and a proposed bimodal mechanism in response to Se status below and above this dietary Se level was identified. The expression of the sepp1 paralogues differed, with only sepp1a responding to Se status. High dietary Se supplementation (30 mg/kg) decreased reproductive success, while the offspring of ZF fed above 0·3 mg Se/kg diet had lower locomotor activity than the other groups. Overall, the novel finding of low selenoprotein expression and activity coinciding with maximum body growth suggests that even small Se-induced variations in redox status may influence cellular growth rates.

  4. Effect of Milking Frequency in Early Lactation on Milk Production, some Blood Metabolites and Reproductive Performance of Holstein Dairy Cow

    Directory of Open Access Journals (Sweden)

    Abolfazl Kiani

    2014-08-01

    Full Text Available The objective of this study was to evaluate the effect of different milking frequencies in the first 6 weeks of lactation on milk production and milk constituents, blood metabolic profiles and reproductive performance of fresh dairy cows. The milking frequencies imposed were three times daily milking for 42 days (3X, six times daily milking for the first 21 days of lactation and three times daily subsequently (6X-3X and six times daily milking for 42 days. For this purpose 21 multiparous Holstein dairy cows were allocated to three groups based on BCS, parity, and body weight. Results showed that the mean of milk and FCM production was significantly higher for 6X than 3X cows in first and second 21 days and in the entire period. Among milk constituent only fat production was affected by milking frequencies. The milking frequency had no effect on mean DMI. Weight loss of the cows was higher for 6X cows (-32 kg than those the 6X-3X (-29 kg and 3X (-29.1 kg. Blood concentration of NEFA was affected by milking frequencies and it was significantly higher for 6X compared to 3X. The mean concentration of blood progesterone and reproductive parameters was not affected by milking frequencies. It was concluded that 6 time milking per day in a short term period may inrease Holstein dairy cows’ performance without any adverse effect on their reproductive parameters.

  5. Naloxone affects reproductive system in a rat model with polycystic features

    Directory of Open Access Journals (Sweden)

    Manizheh Karami

    2015-03-01

    Conclusion: Aspect of rat reproductive system may be linked with the cystic characteristic of ovary. This study involves opioid receptors in the naloxone efficacy on reproductive agents of rat with polycystic aspect.

  6. Does inter-plant variation in sprouting time affect the growth/reproduction trade-off and herbivory in a tropical tree species?

    Directory of Open Access Journals (Sweden)

    Marcilio Fagundes

    Full Text Available ABSTRACT The timing of phenological events varies within and among populations, affecting the performance of individual plants differently. We evaluated the effects of relative variation in sprouting time on the display of reproductive events, vegetative growth and herbivory in Copaifera langsdorffii (Fabaceae. A total of 93 trees of C. langsdorffii was monitored daily to determine their sprouting time. We collected ten terminal branches of each plant to evaluate vegetative growth, production of defense compounds and insect herbivore damage. The sprouting time for the studied population lasted 67 days. Variation in sprouting time did not affect the probability of plants to enter the reproductive stage. Plants that entered the reproductive stage showed greater vegetative biomass. Variation in sprouting time had a negative relationship with branch growth and a positive relationship with the number of leaflets. Leaf phenol concentration did not vary in relation to sprouting time or plant phenology, but herbivory was higher in plants that sprouted later. The relationships among plant sprouting time, vegetative development and display of reproductive stage in C. langsdorffii are discussed. The results of this study also suggest that early sprouting prior to the rainy season is a strategy used by C. langsdorffii to escape herbivores attacks.

  7. Heritability of rectal temperature and genetic correlations with production and reproduction traits in dairy cattle.

    Science.gov (United States)

    Dikmen, S; Cole, J B; Null, D J; Hansen, P J

    2012-06-01

    Genetic selection for body temperature during heat stress might be a useful approach to reduce the magnitude of heat stress effects on production and reproduction. Objectives of the study were to estimate the genetic parameters of rectal temperature (RT) in dairy cows in freestall barns under heat stress conditions and to determine the genetic and phenotypic correlations of rectal temperature with other traits. Afternoon RT were measured in a total of 1,695 lactating Holstein cows sired by 509 bulls during the summer in North Florida. Genetic parameters were estimated with Gibbs sampling, and best linear unbiased predictions of breeding values were predicted using an animal model. The heritability of RT was estimated to be 0.17 ± 0.13. Predicted transmitting abilities for rectal temperature changed 0.0068 ± 0.0020°C/yr from (birth year) 2002 to 2008. Approximate genetic correlations between RT and 305-d milk, fat, and protein yields, productive life, and net merit were significant and positive, whereas approximate genetic correlations between RT and somatic cell count score and daughter pregnancy rate were significant and negative. Rectal temperature during heat stress has moderate heritability, but genetic correlations with economically important traits mean that selection for RT could lead to lower productivity unless methods are used to identify genes affecting RT that do not adversely affect other traits of economic importance.

  8. Chocolate and other cocoa products: effects on human reproduction and pregnancy.

    Science.gov (United States)

    Brillo, Eleonora; Di Renzo, Gian Carlo

    2015-11-18

    Chocolate and other cocoa products are not all alike. They differ between themselves in term of nutrients, calories, and bioactive constituents. Therefore, some of them are unhealthy foods, whereas others do not affect health and still others are healthy foods. One wonders which chocolate and other cocoa derivatives can be considered as biofunctional food products. This review explores the constituents of cocoa and chocolate and summarizes evidence about the role of cocoa and chocolate components on human health and particularly on reproduction. On the basis of the literature review, it can be asserted that some kinds of cocoa products have favorable effects on human health at different stages of life. Women seem to be particularly favored by consuming of cocoa products, and chocolate with specific features can also be a good supplementary source of energy for pregnant woman. However, many aspects remain to be investigated and others are still to be clarified. Future studies and systematic reviews will shed light on some preventive effects and health benefits of cocoa products.

  9. Determination of factors affecting relapse of vaginitis among reproductive-aged women: An experimental study

    Science.gov (United States)

    Parsapour, Roxana; Majlessi, Fereshteh; Rahimiforoushani, Abbas; Sadeghi, Roya

    2017-01-01

    Introduction Vaginitis is a common problem for women, especially in reproductive-aged women. It is a worldwide health problem with many side effects but could be prevented by a health-promoting lifestyle related to vagina health. The aim of this study was to determine the factors affecting relapse of vaginitis. Methods In this experimental study, 350 reproductive-aged women with vaginitis were selected from 10 health centers in Kermanshah (Iran) during 2015 and were equally included in the intervention and control groups. To collect data, a researcher-created questionnaire, which included sociodemographic and health-promoting lifestyle questions, was used. The educational intervention was performed over 20 sessions, each lasting 25–35 minutes. An intervention group was educated by face-to-face education, pamphlets, phone contacts, text messages, and social media. Another group continued the routine clinic education and treatment without contacting the intervention group. Data were analyzed through chi-square and a logistics regression model using IBM-SPSS version 20. Results The results of the study indicated a significant relation between sociodemographic characteristics such as women and their husbands’ literacy, job, family size, income, area for each member of family, tendency of pregnancy, body mass index (BMI), and caesarean experience (plifestyle dimensions and prevention of vaginitis were identified. Relapse after intervention in the intervention group was 27.7% and 72.3% in the control group. According to the logistic regression analysis, chance for relapse of vaginitis in the group that did not receive intervention was more than the same chance in the intervention group (OR=5.14). Conclusion Health-promoting lifestyle intervention influences prevention of vaginitis. Health-promoting lifestyle, literacy promotion, prevention of caesarian, and obesity are beneficial to improvement in lifestyle dimensions associated with vagina health could be

  10. Pharmaceuticals and personal care products: A critical review of the impacts on fish reproduction.

    Science.gov (United States)

    Overturf, Matthew D; Anderson, Jordan C; Pandelides, Zacharias; Beyger, Lindsay; Holdway, Douglas A

    2015-07-01

    Research in environmental toxicology involving pharmaceuticals and personal care products (PPCPs) has increased greatly over the last 10-15 years. Much research has been focused on the endocrine-disrupting potential of PPCPs, as they relate to negative population impacts of aquatic organisms. This review assesses the current data on the reported effects of PPCPs on fish reproduction with an emphasis on fecundity, a predictor of population effects. Studies of both individual PPCPs and PPCP mixtures are presented. As the majority of individual PPCP studies reviewed demonstrate negative effects on fish fecundity, we relate these findings to detected surface water concentrations of these compounds. Very few studies involving PPCP mixtures have been conducted; however, the need for these types of studies is warranted as fish are most likely exposed to mixtures of PPCPs in the wild. In addition, laboratory and field assessments of wastewater treatment plant (WWTP) effluents, a major source of PPCPs, are reviewed. Much of the data provided from these assessments are variable and do not generally demonstrate negative impacts on reproduction, or the studies are unable to directly associate observed effects with WWTP effluents. Finally, future research considerations are outlined to provide an avenue into understanding how wild populations of fish are affected by PPCPs. These considerations are aimed at determining the adaptation potential of fish exposed to mixtures of PPCPs over multiple generations. As global use of PPCPs continually rises, the need to discern the effects of chronic exposure to PPCPs is greatly increased.

  11. Reproductive allocation in plants as affected by elevated carbon dioxide and other environmental changes: a synthesis using meta-analysis and graphical vector analysis.

    Science.gov (United States)

    Wang, Xianzhong; Taub, Daniel R; Jablonski, Leanne M

    2015-04-01

    Reproduction is an important life history trait that strongly affects dynamics of plant populations. Although it has been well documented that elevated carbon dioxide (CO2) in the atmosphere greatly enhances biomass production in plants, the overall effect of elevated CO2 on reproductive allocation (RA), i.e., the proportion of biomass allocated to reproductive structures, is little understood. We combined meta-analysis with graphical vector analysis to examine the overall effect of elevated CO2 on RA and how other environmental factors, such as low nutrients, drought and elevated atmospheric ozone (O3), interacted with elevated CO2 in affecting RA in herbaceous plants. Averaged across all species of different functional groups and environmental conditions, elevated CO2 had little effect on RA (-0.9%). RA in plants of different reproductive strategies and functional groups, however, differed in response to elevated CO2. For example, RA in iteroparous wild species decreased by 8%, while RA in iteroparous crops increased significantly (+14%) at elevated CO2. RA was unaffected by CO2 in plants grown with no stress or in low-nutrient soils. RA decreased at elevated CO2 and elevated O3, but increased in response to elevated CO2 in drought-stressed plants, suggesting that elevated CO2 could ameliorate the adverse effect of drought on crop production to some extent. Our results demonstrate that elevated CO2 and other global environmental changes have the potential to greatly alter plant community composition through differential effects on RA of different plant species and thus affect the dynamics of natural and agricultural ecosystems in the future.

  12. No evidence for external genital morphology affecting cryptic female choice and reproductive isolation in Drosophila.

    Science.gov (United States)

    LeVasseur-Viens, Hélène; Polak, Michal; Moehring, Amanda J

    2015-07-01

    Genitalia are one of the most rapidly diverging morphological features in animals. The evolution of genital morphology is proposed to be driven by sexual selection via cryptic female choice, whereby a female selectively uptakes and uses a particular male's sperm on the basis of male genital morphology. The resulting shifts in genital morphology within a species can lead to divergence in genitalia between species, and consequently to reproductive isolation and speciation. Although this conceptual framework is supported by correlative data, there is little direct empirical evidence. Here, we used a microdissection laser to alter the morphology of the external male genitalia in Drosophila, a widely used genetic model for both genital shape and cryptic female choice. We evaluate the effect of precision alterations to lobe morphology on both interspecific and intraspecific mating, and demonstrate experimentally that the male genital lobes do not affect copulation duration or cryptic female choice, contrary to long-standing assumptions regarding the role of the lobes in this model system. Rather, we demonstrate that the lobes are essential for copulation to occur. Moreover, slight alterations to the lobes significantly reduced copulatory success only in competitive environments, identifying precopulatory sexual selection as a potential contributing force behind genital diversification.

  13. Does last week's alcohol intake affect semen quality or reproductive hormones?

    DEFF Research Database (Denmark)

    Hansen, Mette Lausten; Thulstrup, A M; Bonde, Jens Peter;

    2012-01-01

    The association between last 5 days of alcohol intake, semen quality and reproductive hormones was estimated in this cross-sectional study among 347 men. Conventional semen characteristics, DNA fragmentation index and reproductive hormones (testosterone, estradiol, sex hormone-binding globulin...

  14. Local anthropogenic contamination affects the fecundity and reproductive success of an Arctic amphipod

    DEFF Research Database (Denmark)

    Bach, Lis; Fischer, Astrid; Strand, Jakob

    2010-01-01

    to clean site individuals. These results indicated a cost of living in highly contaminated environments in terms of reduced reproductive success. This study confirms the potential of the benthic amphipod O. pinguis as a bioindicator for assessments of reproductive effects of contaminants in the Arctic...

  15. Gluconeogenic supplements do not affect production, reproductive traits and blood metabolite of holstein cows during the transition period Produtos gliconeogênicos não afetam respostas produtivas, reprodutivas e sangüínea de vacas holandesas em transição

    Directory of Open Access Journals (Sweden)

    Paulo Garcez de Oliveira

    2004-01-01

    Full Text Available The use of gluconeogenic supplements for dairy cows during the transition period has produced contradictory responses in the literature, making it difficult to recommend them. The objective of this trial was to evaluate supplementation with propylene glycol (PG, calcium propionate (CP, and "Dairy Power Drench®" (DR on the transitional period of Holstein cows. Parameters studied were variation of body condition score (BCS, body weight (BW, milk production (MP, reproductive efficiency and nonesterified fatty acid concentration (NEFA. One hundred and sixty five animals from two commercial herds were used. Treatments consisted of: C- Control; DR- administration of "Dairy Power Drench®" (3 applications during postpartum; CP- daily administration of calcium propionate (500 g; PG- daily administration of propylene glycol (500 mL. Variation sources studied were the effect of treatments and blocks as function of farm and parity. No interaction between time (weeks and treatments, or treatment effects, were found for BCS. However, there was a quadratic effect of time. Body weight and its variation were not affected by treatment nor by the time × treatment interaction. However, a quadratic effect of time was observed. An interaction time × treatment was observed on MP, but possible differences within each week were not detected. Treatments also did not affect reproductive efficiency parameters. On average, the number of days to first detected postpartum estrus was 69.5, with 2.23 services per conception and 172.6 days open. The mean concentration of nonesterified fatty acids was 376.6 mmol L-1 and no effect of the interaction time × treatment, or of treatment, was observed. However, a linear effect was observed with time, with a decrease of 48.2 mmol L-1 per week.O uso de produtos gliconeogênicos para vacas leiteiras no período de transição tem gerado respostas contraditórias na literatura, dificultando a sua recomendação. O objetivo deste

  16. Parameters Affecting Solvent Production by Clostridium pasteurianum

    OpenAIRE

    Dabrock, Birgit; Bahl, Hubert; Gottschalk, Gerhard

    1992-01-01

    The effect of pH, growth rate, phosphate and iron limitation, carbon monoxide, and carbon source on product formation by Clostridium pasteurianum was determined. Under phosphate limitation, glucose was fermented almost exclusively to acetate and butyrate independently of the pH and growth rate. Iron limitation caused lactate production (38 mol/100 mol) from glucose in batch and continuous culture. At 15% (vol/vol) carbon monoxide in the atmosphere, glucose was fermented to ethanol (24 mol/100...

  17. NADPH Oxidase-Dependent Superoxide Production in Plant Reproductive Tissues.

    Science.gov (United States)

    Jiménez-Quesada, María J; Traverso, José Á; Alché, Juan de Dios

    2016-01-01

    In the life cycle of a flowering plant, the male gametophyte (pollen grain) produced in the anther reaches the stigmatic surface and initiates the pollen-pistil interaction, an important step in plant reproduction, which ultimately leads to the delivery of two sperm cells to the female gametophyte (embryo sac) inside the ovule. The pollen tube undergoes a strictly apical expansion characterized by a high growth rate, whose targeting should be tightly regulated. A continuous exchange of signals therefore takes place between the haploid pollen and diploid tissue of the pistil until fertilization. In compatible interactions, theses processes result in double fertilization to form a zygote (2n) and the triploid endosperm. Among the large number of signaling mechanisms involved, the redox network appears to be particularly important. Respiratory burst oxidase homologs (Rbohs) are superoxide-producing enzymes involved in a broad range of processes in plant physiology. In this study, we review the latest findings on understanding Rboh activity in sexual plant reproduction, with a particular focus on the male gametophyte from the anther development stages to the crowning point of fertilization. Rboh isoforms have been identified in both the male and female gametophyte and have proven to be tightly regulated. Their role at crucial points such as proper growth of pollen tube, self-incompatibility response and eventual fertilization is discussed.

  18. Eficiência produtiva e reprodutiva em vacas leiteiras Productive and reproductive efficiency in dairy cows

    Directory of Open Access Journals (Sweden)

    Tisa Echevarria Leite

    2001-06-01

    longer in the cows that aborted than in the ones that did not abort. The occurrence of stillbirth (P<0.01 and dystocia (P<0.05 significantly increased IEP in 82.4 days and 136.1 days, respectively. The IPPC was not affected by any of the diseases considered. Milk production was affected by dystocia (P<0.05. Cows that had dystocia produced 380.5kg less milk than normal calving cows. We concluded that preventive procedures to control herd health, criterious clinical diagnosis of post partum disorders and development of data bases with precise recordings for regular analysis of the productive and reproductive parameters may lead to a more economic dairy production.

  19. Conditions during adulthood affect cohort-specific reproductive success in an Arctic-nesting goose population

    Directory of Open Access Journals (Sweden)

    Mitch D. Weegman

    2016-05-01

    Full Text Available Variation in fitness between individuals in populations may be attributed to differing environmental conditions experienced among birth (or hatch years (i.e., between cohorts. In this study, we tested whether cohort fitness could also be explained by environmental conditions experienced in years post-hatch, using 736 lifelong resighting histories of Greenland white-fronted geese (Anser albifrons flavirostris marked in their first winter. Specifically, we tested whether variation in age at first successful reproduction, the size of the first successful brood and the proportion of successful breeders by cohort was explained by environmental conditions experienced on breeding areas in west Greenland during hatch year, those in adulthood prior to successful reproduction and those in the year of successful reproduction, using North Atlantic Oscillation indices as proxies for environmental conditions during these periods. Fifty-nine (8% of all marked birds reproduced successfully (i.e., were observed on wintering areas with young only once in their lifetime and 15 (2% reproduced successfully twice or thrice. Variation in age at first successful reproduction was explained by the environmental conditions experienced during adulthood in the years prior to successful reproduction. Birds bred earliest (mean age 4 when environmental conditions were ‘good’ prior to the year of successful reproduction. Conversely, birds successfully reproduced at older ages (mean age 7 if they experienced adverse conditions prior to the year of successful reproduction. Hatch year conditions and an interaction between those experienced prior to and during the year of successful reproduction explained less (marginally significant variation in age at first successful reproduction. Environmental conditions did not explain variation in the size of the first successful brood or the proportion of successful breeders. These findings show that conditions during adulthood prior to

  20. Conditions during adulthood affect cohort-specific reproductive success in an Arctic-nesting goose population.

    Science.gov (United States)

    Weegman, Mitch D; Bearhop, Stuart; Hilton, Geoff M; Walsh, Alyn; Fox, Anthony David

    2016-01-01

    Variation in fitness between individuals in populations may be attributed to differing environmental conditions experienced among birth (or hatch) years (i.e., between cohorts). In this study, we tested whether cohort fitness could also be explained by environmental conditions experienced in years post-hatch, using 736 lifelong resighting histories of Greenland white-fronted geese (Anser albifrons flavirostris) marked in their first winter. Specifically, we tested whether variation in age at first successful reproduction, the size of the first successful brood and the proportion of successful breeders by cohort was explained by environmental conditions experienced on breeding areas in west Greenland during hatch year, those in adulthood prior to successful reproduction and those in the year of successful reproduction, using North Atlantic Oscillation indices as proxies for environmental conditions during these periods. Fifty-nine (8%) of all marked birds reproduced successfully (i.e., were observed on wintering areas with young) only once in their lifetime and 15 (2%) reproduced successfully twice or thrice. Variation in age at first successful reproduction was explained by the environmental conditions experienced during adulthood in the years prior to successful reproduction. Birds bred earliest (mean age 4) when environmental conditions were 'good' prior to the year of successful reproduction. Conversely, birds successfully reproduced at older ages (mean age 7) if they experienced adverse conditions prior to the year of successful reproduction. Hatch year conditions and an interaction between those experienced prior to and during the year of successful reproduction explained less (marginally significant) variation in age at first successful reproduction. Environmental conditions did not explain variation in the size of the first successful brood or the proportion of successful breeders. These findings show that conditions during adulthood prior to the year of

  1. Wolbachia Affects Reproduction and Population Dynamics of the Coffee Berry Borer (Hypothenemus hampei): Implications for Biological Control

    Science.gov (United States)

    Mariño, Yobana A.; Verle Rodrigues, José C.; Bayman, Paul

    2017-01-01

    Wolbachia are widely distributed endosymbiotic bacteria that influence the reproduction and fitness of their hosts. In recent years the manipulation of Wolbachia infection has been considered as a potential tool for biological control. The coffee berry borer (CBB), Hypothenemus hampei, is the most devastating coffee pest worldwide. Wolbachia infection in the CBB has been reported, but until now the role of Wolbachia in CBB reproduction and fitness has not been tested. To address this issue we reared the CBB in artificial diets with and without tetracycline (0.1% w/v) for ten generations. Tetracycline reduced significantly the relative proportion of Wolbachia in the CBB microbiota from 0.49% to 0.04%. This reduction affected CBB reproduction: females fed with tetracycline had significantly fewer progeny, lower fecundity, and fewer eggs per female. Tetracycline also reduced the population growth rate (λ), net reproductive rate (R0), and mean generation time (T) in CBB; the reduction in population growth was mostly due to variation in fertility, according to life time response experiments (LTREs) analysis. Our results suggest that Wolbachia contribute to the reproductive success of the CBB and their manipulation represents a possible approach to CBB biocontrol mediated by microbiome management. PMID:28085049

  2. Trade-off between migration and reproduction : does a high workload affect body condition and reproductive state?

    NARCIS (Netherlands)

    Schmidt-Wellenburg, Carola A.; Visser, G. Henk; Biebach, Brigitte; Delhey, Kaspar; Oltrogge, Martina; Wittenzellner, Andrea; Biebach, Herbert; Kempenaers, Bart

    2008-01-01

    Migratory birds have to invest much energy into flight to reach their summer and winter quarters. Many studies have shown how migration affects body physiology, including the accumulation of energy stores and the reduction of nonessential organs. In spring, the costs of migration may trade-off with

  3. Does Labor Diversity Affect Firm Productivity?

    DEFF Research Database (Denmark)

    Parrotta, Pierpaolo; Pozzoli, Dario; Pytlikova, Mariola

    Using an employer-employee dataset, we analyze how diversity in cultural background, skills and demographic characteristics a?ects total factor productivity (TFP) of ?rms in Denmark. Implementing structural estimation of ?rms' production function, we ?nd evidence that labor diversity in skills/ed......-average trade openness, giving support to the hypothesis that an ethnically diverse workforce provides information and access to global markets....... and integration costs connected to a more demographically and culturally diverse workforce, counteract the positive e?ects of diversity on ?rm TFP, coming from creativity and knowledge spillovers. However, we ?nd that ethnic diversity is valuable for ?rms operating in industries characterized by above...

  4. Farmer's attitudes affect piglet production parameters

    OpenAIRE

    Kauppinen, T; Valros, A.; Vesala, K.M.

    2010-01-01

    Our results show that farmers' attitudes count: treating the animals humanely, investing in a favourable environment, and having a positive attitude towards new information and scientific research is associated with an above-average productivity on piglet farms. These attitudes, when implemented and concretized in practice, also benefit the animals through a higher standard of welfare.

  5. Serological profile of offspring on an intensive pig farm affected byPorcine reproductive andrespiratory syndrome

    Institute of Scientific and Technical Information of China (English)

    SilvaJ; RochaD; CunhaI; RuiSalesL; NetoF; FontesMC; Simes J

    2015-01-01

    Objective:To evaluate/predict the offspring serum profile of antibodies against Porcine reproductive and respiratory syndrome virus (PRRSV) in an affected intensive herd, whereas sows are vaccinated, and determine the better sample time using pigs as sentinels. Methods:Serum samples were collected from a total of 66 offspring pigs from a PRRSV endemic herd, whereas only sows were vaccinated. Six animals per grouped age were randomly selected with 0 (at perinatal time), 1, 2, 3, 6, 9, 12, 15, 18, 21 and 24 weeks of age. Individual and three pooled samples were tested by ELISA and PCR, respectively.Results: The proportion of seropositive animals was 75.0% (18/24), 33.3% (6/18) and 95.8% (23/24) at farrowing (0 to 3 weeks), nursery (6 to 12 weeks) and growing/finishing (15 to 24 weeks) phases, respectively. It was 46 times more likely (P<0.01) to observe seropositive pigs on the growing/finishing than on nursery phase. European PRSSV strain was detected on serum of 6, 9 and 15-weeks old pigs. Polynomial fit degree 4 regression between sample-to-positive (S/P) ratios and age of pigs(r=0.78; R2= 0.60;P<0.001) estimated a quickly decrease of maternal antibodies, above 0.4 S/P value (cut off), until the 6th week following by a progressive increment of humoral immunity until 21st week of age, when it declines.Conclusions:The results indicate that the piglets can present viremia early at nursery, and the 18th – 21st weeks are the best time to sample offspring pigs, as sentinel, from PRRSV contaminated intensive herds.

  6. Altitude affects the reproductive performance in monoicous and dioicous bryophytes: examples from a Brazilian Atlantic rainforest

    OpenAIRE

    MACIEL-SILVA,ADAÍSES S.; Marques Valio, Ivany F.; Rydin, HÅkan

    2012-01-01

    Species traits, such as breeding system, phylum and growth form and habitat characteristics are shown to influence reproductive performance of liverworts and mosses in the Brazilian Atlantic Rainforest, and drive life-history differentiation among species and populations.

  7. Factors affecting reproductive success of the California clapper rail (Rallus longirostris obsoletus) in San Francisco Bay

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — We assessed the reproductive success of the California clapper rail (Rallus longirostris obsoletus), an endangered species restricted to San Francisco Bay, and the...

  8. Breeding resource distribution affects selection gradients on male phenotypic traits: experimental study on lifetime reproductive success in the bitterling fish (Rhodeus amarus).

    Science.gov (United States)

    Reichard, Martin; Ondracková, Markéta; Bryjová, Anna; Smith, Carl; Bryja, Josef

    2009-02-01

    The spatial distribution of breeding resources can have pronounced demographic and evolutionary consequences. We used 20 experimental groups of the bitterling (Rhodeus amarus), an annual fish with a promiscuous, resource-based mating system, and extended breeding season to investigate how the spatial distribution (clumped or regular) of bitterling oviposition sites (live freshwater mussels) affected offspring production, variation in reproductive success, and directional selection on phenotypic traits over their entire reproductive lifetime. We did not detect any effect of resource distribution on offspring production or variation in reproductive success among individual fish, although variation between replicates was higher with a clumped distribution. This finding is discussed with regard to the incidence of alternative mating behaviors (sneaking) within the limitations imposed by our experimental design. Breeding resource distribution had a significant effect on selection on male phenotypic traits. Stronger directional selection on traits associated with intrasexual competition for fertilizations, gonad mass (an indicator of sperm competition), and the extent of red, carotenoid-based pigment in the iris (an index of dominance status), was detected with a clumped resource distribution. With a regular resource distribution, a stronger positive selection on male body size was detected. We discuss the implications of our results for natural populations.

  9. All eggs are not equal: the maternal environment affects progeny reproduction and developmental fate in Caenorhabditis elegans.

    Directory of Open Access Journals (Sweden)

    Simon C Harvey

    Full Text Available BACKGROUND: Maternal effects on progeny traits are common and these can profoundly alter progeny life history. Maternal effects can be adaptive, representing attempts to appropriately match offspring phenotype to the expected environment and are often mediated via trade-offs between progeny number and quality. Here we have investigated the effect of maternal food availability on progeny life history in the free-living nematode Caenorhabditis elegans. METHODOLOGY/PRINCIPAL FINDINGS: The maternal environment affects both reproductive traits and progeny development. Comparisons of the progeny of worms from high and low maternal food environments indicates that low maternal food availability reduces progeny reproduction in good environments, increases progeny reproduction in poor environments and decreases the likelihood that progeny will develop as dauer larvae. These analyses also indicate that the effects on progeny are not a simple consequence of changes in maternal body size, but are associated with an increase in the size of eggs produced by worms at low maternal food availabilities. CONCLUSIONS/SIGNIFICANCE: These results indicate that the maternal environment affects both progeny reproduction and development in C. elegans and therefore that all progeny are not equal. The observed effects are consistent with changes to egg provisioning, which are beneficial in harsh environments, and of changes to progeny development, which are beneficial in harsh environments and detrimental in benign environments. These changes in progeny life history suggest that mothers in poor quality environments may be producing larger eggs that are better suited to poor conditions.

  10. Male size composition affects male reproductive variance in Atlantic cod Gadus morhua L. spawning aggregations

    DEFF Research Database (Denmark)

    Bekkevold, Dorte

    2006-01-01

    Estimates of Atlantic cod Gadus morhua reproductive success, determined using experimental spawning groups and genetic paternity assignment of offspring, showed that within-group variance in male size correlated positively with the degree of male mating skew, predicting a decrease in male...... reproductive skew with decreasing size variation among males under natural conditions. (c) 2006 The Author Journal compilation (c) 2006 The Fisheries Society of the British Isles...

  11. Productive Parasites: Thinking of Noise as Affect

    Directory of Open Access Journals (Sweden)

    Marie Thompson

    2012-11-01

    Full Text Available Drawing from Michel Serres’ notion of the parasite, this article provides a nuanced explanation for noise that distinguishes itself from prevailing negative narratives, which often seek to define noise as unwanted, undesirable or damaging sound. Such narratives have left noise vulnerable to moralising polemics, which construct silence and noise as a dichotomy between the past and present, natural and cultural, relaxing and disturbing, and, fundamentally, good and bad. This article facilitates a reconsideration of noise’s ethical connotations by proposing the notion of noise as affect.

  12. Effect of stocking rate on pasture production, milk production, and reproduction of dairy cows in pasture-based systems.

    Science.gov (United States)

    Macdonald, K A; Penno, J W; Lancaster, J A S; Roche, J R

    2008-05-01

    Ninety-four cows were randomly allocated to 1 of 5 stocking rates (2.2, 2.7, 3.1, 3.7, and 4.3 cows/ha) in a completely randomized design for 3 years. Herds were seasonal calving, with only minor differences in grazing management to optimize the profitability of each stocking rate (SR). Pasture production and quality data, milk and milk component data, and reproduction data were collected, averaged for SR treatment, and linear and quadratic contrasts on SR were evaluated. In addition, the Wilmink exponential model (y(t) = a + b x e((-0.05t) )+ c x t) was fitted to milk yield within lactation, and the parameters were averaged by SR treatment and analyzed as above. The median variation explained by the function for individual lactations was 84%. The amount of pasture grown tended to increase, and the quality of the pasture on offer increased linearly with increasing SR, reducing some of the negative impact of SR on the availability of pasture per cow. Milk production per cow declined linearly with increasing SR, although there was a tendency for most production variables to decline quadratically, with the negative effect of SR declining with increasing SR. The effect on milk production per cow was primarily because of a lower peak milk yield and a greater post-peak decline (less persistent milk profile), although a decline in lactation length with increasing SR was responsible for 24% of the effect of SR on milk yield. Milk production per hectare increased linearly with increasing SR, and there was only a small difference (approximately 3%/cow per ha) in the efficiency of converting feed dry matter into milk energy. Stocking rate did not affect reproductive success. The data are consistent with the need for a more robust measure of SR than cows per hectare because farms will differ in the genetic merit of their cows and in the potential to produce pasture. We introduce the concept of a comparative SR, whereby the carrying capacity of the farm is defined by the BW of

  13. Study of factors affecting contraceptive use among married women of reproductive Age

    Directory of Open Access Journals (Sweden)

    N Bhandari

    2014-04-01

    Full Text Available Background The use of contraception can prevent pregnancies related complication and helps in improving the women’s health and quality of their lives. Objective To explore reproductive characteristics among married women of reproductive age. To find out method of contraceptive use and side effects among married women of reproductive age. To uncover factors related to contraceptive use among married women of reproductive age. Methods This cross-sectional was carried out among (369 married couples of reproductive age group in ward number 5, 6 and 7 of Dhulikhel Municipality using purposive sampling. Those who fell in between the age group of (14-49 years and willing to participate were included and pregnant women were excluded from the study. Questionnaire was used to obtain informations. Results Among 369(81.3% of the respondents of reproductive age were using a modern contraceptive method. Regarding education 331(89.5% of women’s and 352(95.1% of husbands were literate and 275(91.6% of women were found to be involved in decision making and had good inter-spousal communication that is 280(93.3%. Among the temporary method of family planning, Depo-Provera was the choice 150(54.5% of contraceptive method. Conclusion The present study put more emphasis on increase in women’s literacy, women’s involvement in decision making and inter-spousal communication which helps to promote the effective use of contraceptive methods. As women play an important role in the decision making, women should be included in all aspects of reproductive health and family planning programs. Journal of College of Medical Sciences-Nepal, 2013, Vol-9, No-4, 24-29 DOI: http://dx.doi.org/10.3126/jcmsn.v9i4.10233

  14. Does long-term fungicide exposure affect the reproductive performance of leaf-shredders? A partial life-cycle study using Hyalella azteca.

    Science.gov (United States)

    Baudy, Patrick; Zubrod, Jochen P; Konschak, Marco; Weil, Mirco; Schulz, Ralf; Bundschuh, Mirco

    2017-03-01

    Leaf-shredding amphipods play a critical role in the ecosystem function of leaf litter breakdown, a key process in many low order streams. Fungicides, however, may adversely influence shredders' behavior and the functions they provide, while there is only limited knowledge concerning effects on their reproductive performance. To assess the latter, a semi-static 56-day partial life-cycle bioassay using the model shredder Hyalella azteca (n = 30) was performed applying two environmentally relevant concentrations of a model fungicide mixture (i.e., 5 and 25 μg/L) composed of five fungicides with different modes of toxic action. Variables related to the food processing (leaf consumption and feces production), growth (body length and dry weight), energy reserves (lipid content), and reproduction (amplexus pairs, number and length of offspring) were determined to understand potential implications in the organisms' energy budget. While the fungicides did not affect leaf consumption, both fungicide treatments significantly reduced amphipods' feces production (∼20%) compared to the control. This observation suggests an increased food utilization to counteract the elevated and stress-related energy demand: although growth as well as energy reserves were unaffected, amplexus pairs were less frequently observed in both fungicide treatments (∼50-100%) suggesting a tradeoff regarding energy allocation favoring the maintenance of fundamental functions at the organism level over reproduction. As a result, the time to release of first offspring was delayed in both fungicide treatments (7 and 14 days) and the median number of offspring was significantly lower in the 25-μg/L treatment (100%), whereas offspring length remained unaffected. The results of this study thus indicate that chronic fungicide exposures can negatively impact shredders' reproductive performance. This may translate into lower abundances and thus a reduced contribution to leaf litter breakdown in

  15. Factors affecting unmet need for family planning in married women of reproductive age group in urban slums of Lucknow

    Directory of Open Access Journals (Sweden)

    Anjali Pal

    2014-03-01

    Full Text Available Background: Unmet need for family planning signifies the gap between the reproductive intentions of couples and their actual contraceptive behaviour. The National Family Health Surveys carried out in India in 1992-93, 1998-99 and 2004-2005 have revealed that for a sizable proportion of the population in the reproductive age group, the need for contraceptive services are not met with despite the existence of a National Policy on family planning since 1983. This study was carried out to assess the extent of unmet need for family planning among married women of reproductive age group in urban slums of Lucknow and identify the various factors affecting it. Study design: Cross sectional Setting: four urban slums of Lucknow Participants: 414 married women in the age group of 15- 44 years Study variables: age, education, occupation, religion, parity Statistical analysis: chi- square test, logistic regression analysis, fisher’s exact test Results: the extent of unmet need among married women of reproductive age group was 53.1%. The unmet need was found to be significantly associated with age, number of living sons, discussion of family planning with husband, perception of husband’s view on family planning and husbands’ behaviour towards use of family planning method. Logistic regression analysis of unmet need showed that the lower age of the woman, lesser number of living sons and husband’s discouragement towards the use of FP method were correlated with the unmet need for Family Planning.

  16. Involvement of inositol in reproduction.

    NARCIS (Netherlands)

    Beemster, P.; Groenen, P.; Steegers-Theunissen, R.P.M.

    2002-01-01

    Inositol is involved in several aspects of reproduction. It affects overall embryogenesis, may prevent neural tube defects, and stimulates the production of lung surfactant. This article will review the involvement of inositol in reproduction. After describing the biologic function of inositol and i

  17. Measuring and adapting behavior during product interaction to influence affect

    NARCIS (Netherlands)

    Alonso, M.B.; Hummels, C.C.M.; Keyson, D.V.; Hekkert, P.P.M.

    2011-01-01

    Sometimes, the way in which we interact with products implicitly communicates how we feel. Based on previous studies on how emotions can be detected and communicated via product interaction, we discuss how an interactive product could influence affect by responding and changing behaviors expressing

  18. Effect of neosporosis on productive and reproductive performance of dairy cattle in Costa Rica

    NARCIS (Netherlands)

    Romero, J.J.; Breda, van S.; Vargas, B.; Dolz, G.; Frankena, K.

    2005-01-01

    A cross-sectional study was conducted to assess the effect of neosporosis on productive and reproductive parameters in dairy cows. Cows (n = 2743) from 94 farms located in the most important dairy areas in Costa Rica were used in the study. The size of the herds ranged from 32 to 379 females (mean =

  19. Local anthropogenic contamination affects the fecundity and reproductive success of an Arctic amphipod

    NARCIS (Netherlands)

    Bach, L.; Fischer, A.; Strand, J.

    2010-01-01

    This study investigates whether adaptation to life in contaminated Arctic areas carries a cost for the populations in terms of reduced fecundity and reproductive success. The benthic amphipod, Orchomenella pinguis occurs in huge densities in both clean and contaminated sites. O. pinguis was collecte

  20. Transgenerational interactions involving parental age and immune status affect female reproductive success in Drosophila melanogaster.

    Science.gov (United States)

    Nystrand, M; Dowling, D K

    2014-11-07

    It is well established that the parental phenotype can influence offspring phenotypic expression, independent of the effects of the offspring's own genotype. Nonetheless, the evolutionary implications of such parental effects remain unclear, partly because previous studies have generally overlooked the potential for interactions between parental sources of non-genetic variance to influence patterns of offspring phenotypic expression. We tested for such interactions, subjecting male and female Drosophila melanogaster of two different age classes to an immune activation challenge or a control treatment. Flies were then crossed in all age and immune status combinations, and the reproductive success of their immune- and control-treated daughters measured. We found that daughters produced by two younger parents exhibited reduced reproductive success relative to those of other parental age combinations. Furthermore, immune-challenged daughters exhibited higher reproductive success when produced by immune-challenged relative to control-treated mothers, a pattern consistent with transgenerational immune priming. Finally, a complex interplay between paternal age and parental immune statuses influenced daughter's reproductive success. These findings demonstrate the dynamic nature of age- and immune-mediated parental effects, traceable to both parents, and regulated by interactions between parents and between parents and offspring.

  1. Spring phenology does not affect timing of reproduction in the great tit (Parus major)

    NARCIS (Netherlands)

    Schaper, Sonja V.; Rueda, Carolina; Sharp, Peter J.; Dawson, Alistair; Visser, Marcel E.

    2011-01-01

    Many seasonal breeders adjust the timing of reproduction in response to year-to-year variations in supplementary environmental cues, amongst which ambient temperature is thought to be most influential. However, it is possible that for species such as the great tit (Parus major L.), phenological cues

  2. Social variables affecting mate preferences, copulation and reproductive outcome in a pack of free-ranging dogs.

    Directory of Open Access Journals (Sweden)

    Simona Cafazzo

    Full Text Available Mating and reproductive outcome is often determined by the simultaneous operation of different mechanisms like intra-sexual competition, mating preferences and sexual coercion. The present study investigated how social variables affected mating outcome in a pack of free-ranging dogs, a species supposed to have lost most features of the social system of wolves during domestication. We found that, although the pack comprised multiple breeding individuals, both male copulation success and female reproductive success were positively influenced by a linear combination of dominance rank, age and leadership. Our results also suggest that mate preferences affect mating outcome by reinforcing the success of most dominant individuals. In particular, during their oestrous period bitches clearly searched for the proximity of high-ranking males who displayed affiliative behaviour towards them, while they were more likely to reject the males who intimidated them. At the same time, male courting effort and male-male competition for receptive females appeared to be stronger in the presence of higher-ranking females, suggesting a male preference for dominant females. To our knowledge, these results provide the first clear evidence of social regulation of reproductive activities in domestic dogs, and suggest that some common organizing mechanisms may contribute to shape the social organization of both dogs and wolves.

  3. [Effect of energy intake on production and reproduction characteristics in (breeding) sows].

    Science.gov (United States)

    den Hartog, L A

    1985-04-01

    A total number of 113 first-litter sows and 680 gilts of the Dutch Landrace were used in order to study the effect of energy intake on productive and reproductive characteristics. A high feeding level in normal sows after weaning is essential, not to increase the ovulation rate but rather to improve the condition of the sow and advance oestrus. The experiment with the gilts showed that from the point of view of the cost of the feed consumed and reproductive performance, an energy intake during rearing of more than 2.1 times maintenance will have an adverse effect when adequate protein is given.

  4. Water availability and population origin affect the expression of the tradeoff between reproduction and growth in Plantago coronopus

    DEFF Research Database (Denmark)

    Hansen, C. F.; Garcia, M. B.; Ehlers, B. K.

    2013-01-01

    showed a similar response to drought condition with little variation among maternal families, suggesting a history of selection favouring genotypes with high allocation to reproduction when water availability is low. Plants from annual populations also expressed the highest level of plasticity...... temperature and precipitation. We found that water availability affected the expression of the tradeoff (both phenotypic and genetic) between reproduction and growth, being most accentuated under dry condition. However, populations responded very differently to water treatments. Plants from annual populations...... values similar to those of the annuals, although it was significantly less plastic. We stress the importance of considering intraspecific variation in response to environmental change such as drought, as conspecific plants exhibited very different abilities and strategies to respond to high versus low...

  5. A field survey of some camel productive and reproductive traits in the Butana area, Sudan

    Directory of Open Access Journals (Sweden)

    Babiker E. A,

    2011-08-01

    Full Text Available This field survey study was conducted to investigate major constraints facing camel herd growth and reproduction in the Butana area. Data were collected during the study period from July 2009 to June 2010. Forms of questionnaires distributed, included different productive and reproductive characters, where a total of 321 camels were surveyed. The results showed that the number of pregnant she-camels was found positively correlated with age. Seventy percent of delivered females were recorded during August. General reproductive traits recorded during the survey period revealed that mean age at puberty was 40.5 months, while full reproductive potential was reached at a mean of 60 months. Age at first calving was recorded to be 72 months, while calving interval was observed at a mean of 30.5 months. Daily milk yield surveyed was higher (P<0.05 during winter and autumn (3.16 ± 2.41 and 2.88 ± 2.41 L, respectively, while lower daily milk production was recorded during summer (1.23 ± 1.22 L. Milk production was found positively correlated with age.

  6. Reproduction of confused flour beetle Tribolium confusum Du Val (Coleoptera: Tenebrionidae on common and spelt wheat and their products

    Directory of Open Access Journals (Sweden)

    Radmila Almaši

    2014-09-01

    Full Text Available The interest in spelt wheat production has grown in recent years but there is hardly any information on pest development on that wheat species in storehouses. The influence of common and spelt wheat and their products on the reproduction and offspring of confused flour beetles Tribolium confusum Du Val was studied under laboratory conditions (22-25 °C and 40-60% RH. The experiment was carried out in four replications with four, 10 and 20 insects over a period of six months. The reproduction of confused flour beetles significantly varied depending on the wheat species and product. The highest reproduction rate was recorded on spelt wheat (Triticum spelta L.. The greatest number of offspring beetles appeared on spelt wheat flour (23469. The number of offspring beetles was higher on common (7044 than on spelt wheat kernels (5469. No offspring developed on common wheat pasta while only 4 young beetles were found on spelt wheat pasta. Offspring numbers increased with storage period up to a point but further on they depended on insect number. The number of insects increased over the first four months but decreased later on and the mortality rate was higher. Initial population density affected the offspring numbers but offspring numbers did not rise proportionally with the rising initial population density. Considering the species of wheat, higher mortality was recorded in common wheat. Regarding the type of product, the highest mortality was recorded in pasta, then in kernels and the lowest in flour. The paper shows that confused flour beetles develop extremely well on spelt wheat, even better than on common wheat which is widely grown in Serbia.

  7. Impact of swine reproductive technologies on pig and global food production.

    Science.gov (United States)

    Knox, Robert V

    2014-01-01

    Reproductive technologies have dramatically changed the way pigs are raised for pork production in developed and developing countries. This has involved such areas as pigs produced/sow, more consistent pig flow to market, pig growth rate and feed efficiency, carcass yield and quality, labor efficiency, and pig health. Some reproductive technologies are in widespread use for commercial pork operations [Riesenbeck, Reprod Domest Anim 46:1-3, 2011] while others are in limited use in specific segments of the industry [Knox, Reprod Domest Anim 46:4-6, 2011]. Significant changes in the efficiency of pork production have occurred as a direct result of the use of reproductive technologies that were intended to improve the transfer of genes important for food production [Gerrits et al., Theriogenology 63:283-299, 2005]. While some technologies focused on the efficiency of gene transfer, others addressed fertility and labor issues. Among livestock species, pig reproductive efficiency appears to have achieved exceptionally high rates of performance (PigCHAMP 2011) [Benchmark 2011, Ames, IA, 12-16]. From the maternal side, this includes pigs born per litter, farrowing rate, as well as litters per sow per year. On the male side, boar fertility, sperm production, and sows served per sire have improved as well [Knox et al., Theriogenology, 70:1202-1208, 2008]. These shifts in the efficiency of swine fertility have resulted in the modern pig as one of the most efficient livestock species for global food production. These reproductive changes have predominantly occurred in developed countries, but data suggests transfer and adoption of these in developing countries as well (FAO STAT 2009; FAS 2006) [World pig meat production: food and agriculture organization of the United Nations, 2009; FAS, 2006) Worldwide Pork Production, 2006]. Technological advancements in swine reproduction have had profound effects on industry structure, production, efficiency, quality, and profitability. In

  8. Reproductive responses and productive characteristics in ewes supplemented with detoxified castor meal for a long period

    Directory of Open Access Journals (Sweden)

    Liliane Moreira Silva

    2014-08-01

    Full Text Available The objective of the present study was to evaluate the effects of supplementation with detoxified castor meal (DCM in the diet of ewes during pregnancy, partum, and post-partum on the weight development of their offspring and at slaughter. The study included 56 ewes with synchronized estrus that were naturally mated. At the beginning of pregnancy and in post-partum, hepatic and renal function-related parameters and progesterone levels were measured. At slaughter, the proximate composition and fatty acid profile were determined in the loin of ewes. There was no effect of diet on reproductive response after estrus synchronization. At the beginning of pregnancy, albumin and creatinine levels were lower in the DCM group. Supplementation with DCM did not alter the weight or body condition of ewes at partum. However, at weaning, the DCM group showed a higher loin-eye area (LEA in relation to the group fed diets without detoxified castor meal (WDCM. At partum, as well as at weaning, the offspring of the ewes supplemented with DCM had a larger LEA than the WDCM group. In post-partum, levels of glucose, urea, protein, and cholesterol were lower in the DCM group. The return to cyclicity was similar in both groups, with an average of 47 days after partum. At slaughter, neither anatomical and carcass components nor the results of the proximate analysis were affected by the type of diet, except for an increase in heptadecanoic acid in the DCM group. Supplementation with detoxified castor meal in the diet of ewes does not affect lambing, pregnancy, prolificacy, return to cyclicity, milk production, blood biochemical parameters, or carcass characteristics.

  9. THE ESTROGENIC AND ANTIANDROGENIC PESTICIDE METHOXYCHLOR ALTERS THE REPRODUCTIVE TRACT AND BEHAVIOR WITHOUT AFFECTING PITUITARY SIZE OR LH AND PROLACTIN SECRETION IN MALE RATS

    Science.gov (United States)

    The estrogenic and antiandrogenic pesticide methoxychlor alters the reproductive tract and behavior without affecting pituitary size or LH and prolactin secretion in male rats.Gray LE Jr, Ostby J, Cooper RL, Kelce WR.Endocrinology Branch, United States Environment...

  10. Trace concentrations of imazethapyr (IM) affect floral organs development and reproduction in Arabidopsis thaliana: IM-induced inhibition of key genes regulating anther and pollen biosynthesis.

    Science.gov (United States)

    Qian, Haifeng; Li, Yali; Sun, Chongchong; Lavoie, Michel; Xie, Jun; Bai, Xiaocui; Fu, Zhengwei

    2015-01-01

    Understanding how herbicides affect plant reproduction and growth is critical to develop herbicide toxicity model and refine herbicide risk assessment. Although our knowledge of herbicides toxicity mechanisms at the physiological and molecular level in plant vegetative phase has increased substantially in the last decades, few studies have addressed the herbicide toxicity problematic on plant reproduction. Here, we determined the long-term (4-8 weeks) effect of a chiral herbicide, imazethapyr (IM), which has been increasingly used in plant crops, on floral organ development and reproduction in the model plant Arabidopsis thaliana. More specifically, we followed the effect of two IM enantiomers (R- and S-IM) on floral organ structure, seed production, pollen viability and the transcription of key genes involved in anther and pollen development. The results showed that IM strongly inhibited the transcripts of genes regulating A. thaliana tapetum development (DYT1: DYSFUNCTIONAL TAPETUM 1), tapetal differentiation and function (TDF1: TAPETAL DEVELOPMENT AND FUNCTION1), and pollen wall formation and developments (AMS: ABORTED MICROSPORES, MYB103: MYB DOMAIN PROTEIN 103, MS1: MALE STERILITY 1, MS2: MALE STERILITY 2). Since DYT1 positively regulates 33 genes involved in cell-wall modification (such as, TDF1, AMS, MYB103, MS1, MS2) that can catalyze the breakdown of polysaccharides to facilitate anther dehiscence, the consistent decrease in the transcription of these genes after IM exposure should hamper anther opening as observed under scanning electron microscopy. The toxicity of IM on anther opening further lead to a decrease in pollen production and pollen viability. Furthermore, long-term IM exposure increased the number of apurinic/apyrimidinic sites (AP sites) in the DNA of A. thaliana and also altered the DNA of A. thaliana offspring grown in IM-free soils. Toxicity of IM on floral organs development and reproduction was generally higher in the presence of the R

  11. Factors Affecting Parent-Adolescent Discussion on Reproductive Health Issues in Harar, Eastern Ethiopia: A Cross-Sectional Study

    Directory of Open Access Journals (Sweden)

    Tesfaye Assebe Yadeta

    2014-01-01

    Full Text Available Background. Open family discussion on reproductive health (RH issues often leads to increased awareness on RH matters and reduces risky behaviors among adolescents. This study was conducted to assess factors affecting parent-adolescent discussion on RH issues in Harar, Ethiopia. Methods. A cross-sectional survey using face to face interview supplemented with focus group discussion (FGD was conducted on 751 randomly selected parents of 10–19-year-old adolescents. Data was analyzed using SPSS version 15. Results. More than one-fourth (28.76% of parents reported discussing RH issues with their adolescents during the last six months. In the logistic regression, parents who have demonstrated good RH knowledge and positive attitude towards RH were almost six times and seventy percent (AOR 5.69, 95% CI: 3.67–8.82; AOR 1.70, 95% CI: 1.08–2.68 higher in discussing RH with their adolescents than their counterparts, respectively. Conclusion. Parent-adolescent discussion about RH issues rarely occurs and is bounded by lack of knowledge, sociocultural norms, and parental concern that discussion would encourage premarital sex. Reproductive health programs should target on improving awareness of parents and addressing sociocultural norms surrounding reproductive health issues.

  12. The plot thickens: does low density affect visitation and reproductive success in a perennial herb, and are these effects altered in the presence of a co-flowering species?

    Science.gov (United States)

    Feldman, Tracy S

    2008-07-01

    Plants may experience reduced reproductive success at low densities, due to lower numbers of pollinator visits or reduced visit quality. Co-occurring plant species that share pollinators have the potential to facilitate pollination by either increasing numbers of pollinator visits or increasing the quality of visits, but also have the potential to reduce plant reproductive success through competition for pollination. I used a field experiment with a common distylous perennial (Piriqueta caroliniana) in the presence and absence of a co-flowering species (Coreopsis leavenworthii) in plots with one of four different distances between conspecific plants. I found strong negative effects of increasing interplant distance (related to conspecific density) on several components of P. caroliniana reproductive success: pollinator visits to plants per plot visit, visits received by individual plants, conspecific pollen grains on stigmas, outcross pollen grains on stigmas, and probability of fruit production. Although P. caroliniana and C. leavenworthii share pollinators, the co-flowering species did not affect visitation, pollen receipt or reproductive effort in P. caroliniana. Pollinators moved very infrequently between species in this experiment, so floral constancy might explain the lack of effect of the co-flowering species on P. caroliniana reproductive success at low densities. In co-occurring self-incompatible plants with floral rewards, reproductive success at low density may depend more on conspecific densities than on the presence of other species.

  13. The importance of localized auxin production for morphogenesis of reproductive organs and embryos in Arabidopsis.

    Science.gov (United States)

    Robert, Hélène S; Crhak Khaitova, Lucie; Mroue, Souad; Benková, Eva

    2015-08-01

    Plant sexual reproduction involves highly structured and specialized organs: stamens (male) and gynoecia (female, containing ovules). These organs synchronously develop within protective flower buds, until anthesis, via tightly coordinated mechanisms that are essential for effective fertilization and production of viable seeds. The phytohormone auxin is one of the key endogenous signalling molecules controlling initiation and development of these, and other, plant organs. In particular, its uneven distribution, resulting from tightly controlled production, metabolism and directional transport, is an important morphogenic factor. In this review we discuss how developmentally controlled and localized auxin biosynthesis and transport contribute to the coordinated development of plants' reproductive organs, and their fertilized derivatives (embryos) via the regulation of auxin levels and distribution within and around them. Current understanding of the links between de novo local auxin biosynthesis, auxin transport and/or signalling is presented to highlight the importance of the non-cell autonomous action of auxin production on development and morphogenesis of reproductive organs and embryos. An overview of transcription factor families, which spatiotemporally define local auxin production by controlling key auxin biosynthetic enzymes, is also presented.

  14. Honey and honey-based sugars partially affect reproductive trade-offs in parasitoids exhibiting different life-history and reproductive strategies

    NARCIS (Netherlands)

    Harvey, J.A.; Essens, Tijl; Las, Rutger; van Veen, Cindy; Visser, B.; Ellers, Jacintha; Heinen, R.; Gols, Rieta

    2017-01-01

    Adult dietary regimes in insects may affect egg production, fecundity and ultimately fitness. This is especially relevant in parasitoid wasps where many species serve as important biological control agents of agricultural pests. Here, we tested the effect of honey and sugar diets on daily fecundity

  15. Parthenogenetic reproduction of Diaphanosoma celebensis (Crustacea: Cladocera): influence of salinity on feeding, survival, growth and neonate production

    Digital Repository Service at National Institute of Oceanography (India)

    Achuthankutty, C.T.; Shrivastava, Y.; Mahambre, G.G.; Goswami, S.C.; Madhupratap, M.

    to 30 psu. Salinity variations also affected the size and age of primiparous females. Resting egg formation and sexual reproduction did not occur at the tested salinities. The results indicate that D. celebensis is adapted to low saline, estuarine...

  16. Mutations of the Drosophila myosin regulatory light chain affect courtship song and reduce reproductive success.

    Science.gov (United States)

    Chakravorty, Samya; Vu, Hien; Foelber, Veronica; Vigoreaux, Jim O

    2014-01-01

    The Drosophila indirect flight muscles (IFM) rely on an enhanced stretch-activation response to generate high power output for flight. The IFM is neurally activated during the male courtship song, but its role, if any, in generating the small amplitude wing vibrations that produce the song is not known. Here, we examined the courtship song properties and mating behavior of three mutant strains of the myosin regulatory light chain (DMLC2) that are known to affect IFM contractile properties and impair flight: (i) Dmlc2(Δ2-46) (Ext), an N-terminal extension truncation; (ii) Dmlc2(S66A,S67A) (Phos), a disruption of two MLC kinase phosphorylation sites; and (iii) Dmlc2(Δ2-46;S66A,S67A) (Dual), expressing both mutations. Our results show that the Dmlc2 gene is pleiotropic and that mutations that have a profound effect on flight mechanics (Phos and Dual) have minimal effects on courtship song. None of the mutations affect interpulse interval (IPI), a determinant of species-specific song, and intrapulse frequency (IPF) compared to Control (Dmlc2 (+) rescued null strain). However, abnormalities in the sine song (increased frequency) and the pulse song (increased cycles per pulse and pulse length) evident in Ext males are not apparent in Dual males suggesting that Ext and Phos interact differently in song and flight mechanics, given their known additive effect on the latter. All three mutant males produce a less vigorous pulse song and exhibit impaired mating behavior compared to Control males. As a result, females are less receptive to Ext, Phos, and Dual males when a Control male is present. These results open the possibility that DMLC2, and perhaps contractile protein genes in general, are partly under sexual selection. That mutations in DMLC2 manifest differently in song and flight suggest that this protein fulfills different roles in song and flight and that stretch activation plays a smaller role in song production than in flight.

  17. Pathological and anatomical abnormalities affecting buffalo cows reproductive tracts in Mosul

    Directory of Open Access Journals (Sweden)

    O. I. Azawi

    2008-01-01

    Full Text Available A study was conducted to assess the type and prevalence of abnormalities occurring in the female reproductive tracts of 405 buffalo cows slaughtered at Mosul abattoir. Out of the 405 buffalo genital tracts examined, various abnormalities with different degrees of severity were observed in 216 (53.3% of cases. Twenty two (5.4% were pregnant and the remaining 41.2% (167/405 were macroscopically normal. The most common abnormalities encountered were endometritis 50 (12.3%, ovarobursal adhesions 26 (6.4% and hydrosalpinx 20 (4.9%. Other abnormalities recorded were follicular cyst, luteal cyst, cystic corpus luteum, paraovarian cyst, ovarian sarcoma, inactive ovaries, senility anestrous, pyosalpinx, hemosalpinx, obstruction of oviduct, salpingitis, double oviduct, hydrometra, mucometra, pyometra, permetritis, parametritis, uterine edema, perimetrial adhesions, parametrial adhesions, parauterine abscess and uterine tumor. Histopathological examinations in this study revealed that reproductive tract lesions seem to be an important problem with possible subsequent infertility and sterility in buffalo cows leading to animals slaughtered.

  18. Alloparenting experience affects future parental behavior and reproductive success in prairie voles (Microtus ochrogaster).

    Science.gov (United States)

    Stone, Anita Iyengar; Mathieu, Denise; Griffin, Luana; Bales, Karen Lisa

    2010-01-01

    Various hypotheses have been proposed to explain the function of alloparental behavior in cooperatively breeding species. We examined whether alloparental experience as juveniles enhanced later parental care and reproductive success in the prairie vole (Microtus ochrogaster), a cooperatively breeding rodent. Juveniles cared for one litter of siblings (1EX), two litters of siblings (2EX) or no siblings (0EX). As adults, these individuals were mated to other 0EX, 1EX or 2EX voles, yielding seven different pair combinations, and we recorded measures of parental behaviors, reproductive success, and pup development. As juveniles, individuals caring for siblings for the first time were more alloparental; and as adults, 0EX females paired with 0EX males spent more time in the nest with their pups. Taken together, these results suggest that inexperienced animals spend more time in infant care. As parents, 1EX males spent more time licking their pups than 2EX and 0EX males. Pups with either a 1EX or 2EX parent gained weight faster than pups with 0EX parents during certain developmental periods. While inexperienced animals may spend more time in pup care, long-term benefits of alloparenting may become apparent in the display of certain, particularly important parental behaviors such as licking pups, and in faster weight gain of offspring.

  19. Retrospective surveillance of metabolic parameters affecting reproductive performance of Japanese Black breeding cows

    Science.gov (United States)

    Watanabe, Urara; Yamato, Osamu; Otoi, Takeshige; Okamoto, Koji

    2014-01-01

    This retrospective study was conducted to confirm the relationship between pre- and postpartum metabolic parameters and postpartum reproductive performance and to clarify seasonal characteristics of the metabolic parameters by using our metabolic profile test (MPT) database of Japanese Black breeding herds. In evaluation 1, MPT databases of blood samples from multiparous cows collected prepartum and postpartum were divided into two groups according to calving interval, and each MPT parameter was compared. In evaluation 2, the same MPT databases used in evaluation 1 were divided into two groups according to the sampling period. Significant differences were found in the prepartal total protein and postpartal γ-glutamyltransferase in evaluation 1. In evaluation 2, significant differences were found in the prepartal and postpartal total protein, albumin/globulin ratio, and glucose. Clear seasonal differences in MPT results emphasized the usefulness of the MPT in breeding cattle herds fed home-pasture roughage and suggest that unsatisfactory reproductive performance during hot periods reflects inadequate nutritional content of the diet and possible reduced feed intake due to heat stress. PMID:24675835

  20. Methuselah-like genes affect development, stress resistance, lifespan and reproduction in Tribolium castaneum.

    Science.gov (United States)

    Li, Chengjun; Zhang, Yi; Yun, Xiaopei; Wang, Yanyun; Sang, Ming; Liu, Xing; Hu, Xingxing; Li, Bin

    2014-10-01

    Methuselah (Mth) is associated with lifespan, stress resistance and reproduction in Drosophila melanogaster, but Mth is not present in nondrosophiline insects. A number of methuselah-likes (mthls) have been identified in nondrosophiline insects, but it is unknown whether the functions of mth are shared by mthls or are divergent from them. Five mthls have been identified in Tribolium castaneum. Although they have different developmental expression patterns, they all enhance resistance to starvation. Only mthl1 and mthl2 enhance resistance to high temperature, whereas mthl4 and mthl5 negatively regulate oxidative stress in T. castaneum. Unlike in the fly with mth mutation, knockdown of mthls, except mthl3, shortens the lifespan of T. castaneum. Moreover, mthl1 and mthl2 are critical for Tribolium development. mthl1 plays important roles in larval and pupal development and adult eclosion, while mthl2 is required for eclosion. Moreover, mthl1 and mthl2 silencing reduces the fertility of T. castaneum, and mthl1 and mthl4 are also essential for embryo development. In conclusion, mthls have a significant effect on insect development, lifespan, stress resistance and reproduction. These results provide experimental evidence for functional divergence among mthls/mth and clues for the signal transduction of Mthls.

  1. An abbreviated repeat dose and reproductive/developmental toxicity test for high production volume chemicals

    Energy Technology Data Exchange (ETDEWEB)

    Scala, R.A.; Bevan, C.; Beyer, B.K. (Exxon Biomedical Sciences, Inc., East Millstone, NJ (United States))

    1992-08-01

    A novel protocol is described for obtaining preliminary data on repeated dose systemic effects and reproductive/developmental toxicity. The test protocol was developed by a group of experts at the request of the U.S. Environmental Protection Agency (EPA) for use as part of a Screening Information Data Set on high production volume chemicals. Interest in this protocol is shared by several regulatory agencies, including the Organization for Economic Cooperation, the European Community, and the EPA. To validate the study protocol, ethylene glycol monomethyl ether (EGME) was used. After a dosing period of approximately 6 weeks, EGME showed both systemic and reproductive/developmental effects similar to those previously reported using standard protocols. Thus, this test protocol may be used as a screening tool for high production volume chemicals.

  2. Effects of dystocia on milk production and reproduction in subsequent lactation in a Turkish Holstein herd

    OpenAIRE

    İbrahim KAYA; UZMAY, Can; AYYILMAZ, Tarık

    2015-01-01

    The study investigated effects of dystocia on milk production, reproduction, calf loss, and cow culling in a Turkish Holstein herd at the Agricultural Faculty, Ege University, İzmir, Turkey. Data were from 653 single calvings between 2006 and 2010. Considering a greater dystocia incidence in primiparous cows, data was divided in two; 223 primiparous and 430 multiparous calvings scored on a 1-to-5 scale. Scores were categorized into no dystocia and dystocia. With few related observations, all ...

  3. Mercury concentrations in fish-eating birds from the Pinchi Lake area in relation to productivity and reproductive success

    Energy Technology Data Exchange (ETDEWEB)

    Weech, S.A. [Minnow Environmental Inc., Victoria, BC (Canada); Scheuhammer, T.M. [Environment Canada, Ottawa, ON (Canada)]|[Carleton Univ., Ottawa, ON (Canada). National Wildlife Research Centre

    2006-07-01

    A known bird breeding area along the Pinchi fault in central British Columbia is a source of geologic mercury (Hg) in the form of cinnabar. Lakes with a range of sediment Hg concentrations are also present. This study examined the extent to which inorganic Hg from local geologic sources is methylated and incorporated into the food chain of birds feeding on fishes. The possible toxic effects of natural and mining-related Hg releases on local piscivorous breeding bird populations were also studied. Bald eagles and red-necked grebes were monitored from 2000 to 2002 in an effort to determine if increased Hg concentrations were affecting reproductive success and productivity. Fish tissues were collected from rainbow trout and northern pike minnow to determine if Hg levels were elevated. Eagles breeding on 5 lakes were sampled for blood- and feather-Hg concentrations. Red-necked grebe eggs were also collected. All samples were analyzed for total Hg. It was shown that Hg does not appear to have any obvious adverse effects on reproduction. Concentrations of Hg in all grebe eggs were below 0.5 {mu}g.g wet weight, the lowest observed adverse effect level (LOAEL) for Hg developmental toxicity in birds. Although the level of Hg in tissues of Pinchi Lake birds was elevated, the birds were in excellent condition and showed similar reproductive success and productivity to reference populations. This study showed that concurrent tissue sampling and population monitoring can provide a helpful way to prove or refute findings that may indicate a potential contaminant effect on local wildlife. 27 refs., 1 tab., 4 figs.

  4. A review of sexually transmitted bovine trichomoniasis and campylobacteriosis affecting cattle reproductive health.

    Science.gov (United States)

    Michi, Aubrey N; Favetto, Pedro H; Kastelic, John; Cobo, Eduardo R

    2016-03-15

    The objective is to discuss sexually transmitted diseases caused by Tritrichomonas foetus (T foetus) and Campylobacter fetus (C fetus) subsp. venerealis, with a focus on prevalence, pathogenesis, and diagnosis in cows and bulls. Diagnosis and control are problematic because these diseases cause severe reproductive losses in cows, but in bulls are clinically asymptomatic, which allows the disease to flourish, especially in the absence of legislated control programs. We review research regarding prophylactic systemic immunization of bulls and cows with antigens of T foetus and C fetus venerealis and their efficacy in preventing or clearing preexisting infections in the genital tract. Current diagnostic methods of C fetus venerealis and T foetus (microbial culture and PCR) should be improved. Review of the latest advances in bovine trichomoniasis and campylobacteriosis should promote knowledge and provide an impetus to pursue further efforts to control bovine sexually transmitted diseases.

  5. Naloxone affects reproductive system in a rat model with polycystic features

    Institute of Scientific and Technical Information of China (English)

    Manizheh Karami; Maryam Darban Fooladi

    2015-01-01

    Objective: To make interaction between morphine and naloxone in the rat model of PCOS, we evidenced the opioid receptors involvement in this efficacy. Methods:A total of 48 female animals (Wistar rats weighing 200-250 g) were kept diestrous before experimental procedure began. They were grouped in single L-arginine (50 mg/kg, i.p., once a day for 9 days), naloxone (0.4 mg/kg, i.p., once daily for 9 days), morphine (5 mg/kg, i.p., once a day for 9 days), and naloxone (0.4 mg/kg) pre-treated to L-arginine (50 mg/kg), morphine (5 mg/kg) pre-treated to L-arginine, morphine pre-treated to the collective naloxone-L-arginine. Control group solely received saline (1 mL/kg, once daily for 9 days). At the end of the treatment period all animals were surgically studied. The rats’ ovaries and uteri were examined both biometrically and pathologically. Results:The ovaries of rats treated with L-arginine showed polycystic characteristics and their uteri illustrated inflammation changes to the controls. The samples obtained from rats pre-treated with naloxone revealed a decrease in sign of inflammation compaired with L-arginine received speciments, the signs got worse in the presence of the morphine. Conclusion:Aspect of rat reproductive system may be linked with the cystic characteristic of ovary. This study involves opioid receptors in the naloxone efficacy on reproductive agents of rat with polycystic aspect.

  6. Reproductive traits affect the rescue of valuable and endangered multipurpose tropical trees.

    Science.gov (United States)

    Sinébou, Viviane; Quinet, Muriel; Ahohuendo, Bonaventure C; Jacquemart, Anne-Laure

    2016-01-01

    Conservation strategies are urgently needed in Tropical areas for widely used tree species. Increasing numbers of species are threatened by overexploitation and their recovery might be poor due to low reproductive success and poor regeneration rates. One of the first steps in developing any conservation policy should be an assessment of the reproductive biology of species that are threatened by overexploitation. This work aimed to study the flowering biology, pollination and breeding system of V. doniana, a multipurpose threatened African tree, as one step in assessing the development of successful conservation strategies. To this end, we studied (1) traits directly involved in pollinator attraction like flowering phenology, flower numbers and morphology, and floral rewards; (2) abundance, diversity and efficiency of flower visitors; (3) breeding system, through controlled hand-pollination experiments involving exclusion of pollinators and pollen from different sources; and (4) optimal conditions for seed germination. The flowering phenology was asynchronous among inflorescences, trees and sites. The flowers produced a large quantity of pollen and nectar with high sugar content. Flowers attracted diverse and abundant visitors, counting both insects and birds, and efficient pollinators included several Hymenoptera species. We detected no spontaneous self-pollination, indicating a total dependence on pollen vectors. Vitex doniana is self-compatible and no inbreeding depression occurred in the first developmental stages. After extraction of the seed from the fruit, seed germination did not require any particular conditions or pre-treatments and the seeds showed high germination rates. These pollination and breeding characteristics as well as germination potential offer the required conditions to develop successful conservation strategies. Protection, cultivation and integration in agroforestry systems are required to improve the regeneration of the tree.

  7. Relations between affective music and speech: Evidence from dynamics of affective piano performance and speech production

    Directory of Open Access Journals (Sweden)

    Xiaoluan eLiu

    2015-07-01

    Full Text Available This study compares affective piano performance with speech production from the perspective of dynamics: unlike previous research, this study uses finger force and articulatory effort as indexes reflecting the dynamics of affective piano performance and speech production respectively. Moreover, for the first time physical constraints such as piano fingerings and speech articulatory distance are included due to their potential contribution to different patterns of dynamics. A piano performance experiment and speech production experiment were conducted in four emotions: anger, fear, happiness and sadness. The results show that in both piano performance and speech production, anger and happiness generally have high dynamics while sadness has the lowest dynamics, with fear in the middle. Fingerings interact with fear in the piano experiment and articulatory distance interacts with anger in the speech experiment, i.e., large physical constraints produce significantly higher dynamics than small physical constraints in piano performance under the condition of fear and in speech production under the condition of anger. Using production experiments, this study firstly supports previous perception studies on relations between affective music and speech. Moreover, this is the first study to show quantitative evidence for the importance of considering motor aspects such as dynamics in comparing music performance and speech production in which motor mechanisms play a crucial role.

  8. Relations between affective music and speech: evidence from dynamics of affective piano performance and speech production.

    Science.gov (United States)

    Liu, Xiaoluan; Xu, Yi

    2015-01-01

    This study compares affective piano performance with speech production from the perspective of dynamics: unlike previous research, this study uses finger force and articulatory effort as indexes reflecting the dynamics of affective piano performance and speech production respectively. Moreover, for the first time physical constraints such as piano fingerings and speech articulatory constraints are included due to their potential contribution to different patterns of dynamics. A piano performance experiment and speech production experiment were conducted in four emotions: anger, fear, happiness and sadness. The results show that in both piano performance and speech production, anger and happiness generally have high dynamics while sadness has the lowest dynamics. Fingerings interact with fear in the piano experiment and articulatory constraints interact with anger in the speech experiment, i.e., large physical constraints produce significantly higher dynamics than small physical constraints in piano performance under the condition of fear and in speech production under the condition of anger. Using production experiments, this study firstly supports previous perception studies on relations between affective music and speech. Moreover, this is the first study to show quantitative evidence for the importance of considering motor aspects such as dynamics in comparing music performance and speech production in which motor mechanisms play a crucial role.

  9. [Affective disorders in women with reproductive cancer (to the problem of somatoreactive cyclothymia)].

    Science.gov (United States)

    Samushiya, M A; Barinov, V V

    2013-01-01

    Based on the observation of 26 female patients, authors present a specific subtype of bipolar disorder - a somatoreactive cyclothymia which develops concomitantly with cancer. The affective disorder manifests itself as an acceptor of the clinical rhythm of cancer: the first and recurrent affective episodes coincide with the key stages of the disease.

  10. Product Meaning, Affective Use Evaluation, and Transfer: A Preliminary Study

    Directory of Open Access Journals (Sweden)

    Sacha Helfenstein

    2005-01-01

    Full Text Available The first part of this investigation explored the multidimensional nature of product meaning, referring to the variety of connotations and functions a consumer associates with a particular product category. The subsequent experiment examined the moderation effects of product meaning and other attributes of the user on (a the affective evaluation of an obstructed use interaction, and (b the transfer of emotion between devices presented as being either of the same or a different brand. Although the failure experience essentially caused frustration, this reaction varied substantially among consumers depending on product meaning, age, and gender. The results also showed that the emotion dimensions of pleasure, arousal, and dominance were affected in distinct ways, and that, in addition to the consumer variables, transfer was mainly dependent on the brand relation. Nevertheless, user frustration did not cause general brand aversion, indicating that poor designs do not unconditionally threaten the customer relationship.

  11. Product design enhancement using apparent usability and affective quality.

    Science.gov (United States)

    Seva, Rosemary R; Gosiaco, Katherine Grace T; Santos, Ma Crea Eurice D; Pangilinan, Denise Mae L

    2011-03-01

    In this study, apparent usability and affective quality were integrated in a design framework called the Usability Perception and Emotion Enhancement Model (UPEEM). The UPEEM was validated using structural equation modeling (SEM). The methodology consists of four phases namely product selection, attribute identification, design alternative generation, and design alternative evaluation. The first stage involved the selection of a product that highly involves the consumer. In the attribute identification stage, design elements of the product were identified. The possible values of these elements were also determined for use in the experimentation process. Design of experiments was used to identify how the attributes will be varied in the design alternative stage and which of the attributes significantly contribute to affective quality, apparent usability, and desirability in the design evaluation stage. Results suggest that product attributes related to form are relevant in eliciting intense affect and perception of usability in mobile phones especially those directly related to functionality and aesthetics. This study considered only four product attributes among so many due to the constraints of the research design employed. Attributes related to aesthetic perception of a product enhance apparent usability such as those related to dimensional ratios.

  12. Revision of the ICH guideline on detection of toxicity to reproduction for medicinal products: SWOT analysis.

    Science.gov (United States)

    Barrow, Paul

    2016-09-01

    SWOT analysis was used to gain insights and perspectives into the revision of the ICH S5(R2) guideline on detection of toxicity to reproduction for medicinal products. The current ICH guideline was rapidly adopted worldwide and has an excellent safety record for more than 20 years. The revised guideline should aim to further improve reproductive and developmental (DART) safety testing for new drugs. Alternative methods to animal experiments should be used whenever possible. Modern technology should be used to obtain high quality data from fewer animals. Additions to the guideline should include considerations on the following: limit dose setting, maternal toxicity, biopharmaceuticals, vaccines, testing strategies by indication, developmental immunotoxicity, and male-mediated developmental toxicity. Emerging issues, such as epigenetics and the microbiome, will most likely pose challenges to DART testing in the future. It is hoped that the new guideline will be adopted even outside the ICH regions.

  13. Impact of reproductive technologies on dairy food production in the dairy industry.

    Science.gov (United States)

    Stevenson, Jeffrey S

    2014-01-01

    Reproductive technologies drive the efficiency of managing dairy cows because the lactation cycle of the dairy cow depends on regular calving to renew lactation yields. Achieving timely pregnancies to allow calving every 12-14 months, therefore, is critical in modern dairy production. To meet the demands to produce sufficient milk for fluid and dairy products, various technologies are applied to enhance efficiencies on the dairy farm. Artificial insemination (AI), embryo transfer, ultrasonographic and chemical detection of pregnancy, various monitors that detect or predict estrus, and handheld communication and testing devices allow managers to retrieve information to make cow-side decisions about health and reproductive status. Genomic testing of young potential sires or young heifers is now possible and can provide information about their genetic merit years before any progeny tests can be completed. In many countries, the challenge faced by dairy producers is their ability to afford these technologies in the face of rising feed and labor costs and volatile milk prices received at the farm gate. Government policies often place obstacles, trade barriers, and unfunded mandates that preclude operations from making a modest profit. Unlike nearly all other manufacturing industries, agriculture producers have little control over the price received for their products. Therefore, dairy production is vulnerable to many uncontrolled factors including climate, government policy, economic conditions, and skilled labor shortages. It is clear that the impact of emerging and current reproductive technologies is critical to the management of dairy cattle to produce sufficient milk to meet consumer demands for quality fluid and dairy products.

  14. Asexual endophytes in a native grass: tradeoffs in mortality, growth, reproduction, and alkaloid production.

    Science.gov (United States)

    Faeth, Stanley H; Hayes, Cinnamon J; Gardner, Dale R

    2010-10-01

    Neotyphodium endophytes are asexual, seed-borne fungal symbionts that are thought to interact mutualistically with their grass hosts. Benefits include increased growth, reproduction, and resistance to herbivores via endophytic alkaloids. Although these benefits are well established in infected introduced, agronomic grasses, little is known about the cost and benefits of endophyte infection in native grass populations. These populations exist as mosaics of uninfected and infected plants, with the latter often comprised of plants that vary widely in alkaloid content. We tested the costs and benefits of endophyte infections with varying alkaloids in the native grass Achnatherum robustum (sleepygrass). We conducted a 4-year field experiment, where herbivory and water availability were controlled and survival, growth, and reproduction of three maternal plant genotypes [uninfected plants (E-), infected plants with high levels of ergot alkaloids (E+A+), and infected plants with no alkaloids (E+A-)] were monitored over three growing seasons. Generally, E+A+ plants had reduced growth over the three growing seasons and lower seed production than E- or E+A- plants, suggesting a cost of alkaloid production. The reduction in vegetative biomass in E+A+ plants was most pronounced under supplemented water, contrary to the prediction that additional resources would offset the cost of alkaloid production. Also, E+A+ plants showed no advantage in growth, seed production, or reproductive effort under full herbivory relative to E- or E+A- grasses, contrary to the predictions of the defensive mutualism hypothesis. However, E+A+ plants had higher overwintering survival than E+A- plants in early plant ontogeny, suggesting that alkaloids associated with infection may protect against below ground herbivory or harsh winter conditions. Our results suggest that the mosaic of E-, E+A+, and E+A- plants observed in nature may result from varying biotic and abiotic selective factors that maintain

  15. DESTAF: A database of text-mined associations for reproductive toxins potentially affecting human fertility

    KAUST Repository

    Dawe, Adam Sean

    2012-01-01

    The Dragon Exploration System for Toxicants and Fertility (DESTAF) is a publicly available resource which enables researchers to efficiently explore both known and potentially novel information and associations in the field of reproductive toxicology. To create DESTAF we used data from the literature (including over 10. 500 PubMed abstracts), several publicly available biomedical repositories, and specialized, curated dictionaries. DESTAF has an interface designed to facilitate rapid assessment of the key associations between relevant concepts, allowing for a more in-depth exploration of information based on different gene/protein-, enzyme/metabolite-, toxin/chemical-, disease- or anatomically centric perspectives. As a special feature, DESTAF allows for the creation and initial testing of potentially new association hypotheses that suggest links between biological entities identified through the database.DESTAF, along with a PDF manual, can be found at http://cbrc.kaust.edu.sa/destaf. It is free to academic and non-commercial users and will be updated quarterly. © 2011 Elsevier Inc.

  16. Maternally transferred dioxin-like compounds can affect the reproductive success of European eel.

    Science.gov (United States)

    Foekema, Edwin M; Kotterman, Michiel; de Vries, Pepijn; Murk, Albertinka J

    2016-01-01

    Reported concentrations of dioxin-like compounds accumulated in the European eel (Anguilla anguilla) were used to perform a risk assessment for eel larval survival, taking into account a modeled amplification of tissue concentrations with a factor of 1.33 during spawning migration. The calculated concentrations of dioxin-like compounds finally deposited in the eggs were compared with the internal effect concentrations for survival of early life stages of the European eel; these concentrations, by lack of experimental data, were estimated from a sensitivity distribution based on literature data by assuming that eel larvae are among the 10% most sensitive teleost fish species. Given concentrations of dioxin-like contaminants and assuming a relatively high sensitivity, it can be expected that larvae from eggs produced by eel from highly contaminated locations in Europe will experience increased mortality as a result of maternally transferred dioxin-like contaminants. As historical persistent organic pollutant concentrations in eel tissue were higher, this impact must have been stronger in the past. Potential effects of other compounds or effects on the migration, condition, and fertility of the parental animals were not taken into account. It is important to further study the overall impact of contaminants on the reproductive success of the European eel as this may have been underestimated until now.

  17. Can cyanobacterial biomass applied to soil affect survival and reproduction of springtail Folsomia candida?

    Science.gov (United States)

    Lána, Jan; Hofman, Jakub; Bláha, Luděk

    2011-05-01

    Biomass of cyanobacterial water blooms including cyanobacterial toxins may enter soils, for example, when harvested water bloom is directly applied as an organic fertilizer or when water with massive cyanobacterial biomass is used for irrigation. In spite of this, no information is available about the potential effects on soil arthropods. The objective of this pilot study was to evaluate the effects of water bloom biomass sampled in five different fresh water lakes on the soil dwelling arthropod, springtail Folsomia candida (Collembola). These samples contained different dominant species of cyanobacteria and varied significantly in microcystin content (21-3662 μg/g dw biomass). No adverse effects on survival or reproduction were observed for any tested sample at concentration up to 4 g dw biomass/kg dw soil. Despite the known hazardous properties of water blooms in aquatic ecosystems, our pilot results suggest that cyanobacterial biomass might have no significant impact on arthropods in soil. It remains a question, if this is due to low bioavailability of cyanobacterial toxins in soil.

  18. Age and reproductive status of adult Varroa mites affect grooming success of honey bees.

    Science.gov (United States)

    Kirrane, Maria J; de Guzman, Lilia I; Rinderer, Thomas E; Frake, Amanda M; Wagnitz, Jeremy; Whelan, Pádraig M

    2012-12-01

    This study evaluated for the first time the grooming response of honey bees to Varroa mites of different ages and reproductive statuses in the laboratory. Plastic cages containing a section of dark comb and about 200 bees were inoculated with groups of four classes of mites: gravid, phoretic foundresses, phoretic daughters and a combination of gravid and phoretic foundress mites. Each cage received 20 mites belonging to one of these classes. Our results showed that, 1 day after mite inoculation, phoretic daughter mites were the most prone to grooming by honey bees with an average mite drop of 49.8 ± 2.6 %. The lowest mite drop was recorded for bees inoculated with phoretic foundresses (30.3 ± 3.6 %) but was comparable to bees inoculated with gravid mites (31.8 ± 3.8 %) and the combination of gravid and phoretic foundress mites (34.2 ± 3.2 %). No differences among mite types were detected during the second and third days of observation. Regardless of mite type, the highest mite drop was recorded on the first day (35 ± 2.1 %) compared to the drop for any subsequent day (grooming behaviour may increase our insight into the importance of grooming in mite resistance.

  19. An investigation into the factors affecting the natural reproduction of Opsaridium peringueyi

    Science.gov (United States)

    Moyo, N. A. G.

    An endangered freshwater fish, Opsaridium peringueyi, was studied from January, 2009 to December, 2009. The analysis of the environmental conditions indicated that the fish is found in streams with moderate to fast flow, high oxygen levels, a depth greater than 0.6 m and temperatures between 10 and 24 °C. O. peringueyi is sexually dimorphic with males growing at a faster rate and attaining a larger size than females. The breeding biology of this species was investigated in glass aquarium tanks. The spawning behaviour is described for the first time. The breeding colour of the male is deep red on the operculum, ventral part, caudal and ventral fins. The breeding colour in the female is the same as the male except the red colour is lighter. The breeding of O. peringueyi is a four stage process which begins with the appearance of breeding colour culminating in the laying of eggs after courtship. Temperature, flow-rate, conductivity and substrate were identified as the environmental cues important in the reproduction of this species. All these factors had a significant effect on the breeding activity of O. peringueyi. The possible effect of climate change on O. peringueyi is discussed.

  20. Reproductive and health assessment of fathead minnows (Pimephales promelas) inhabiting a pond containing oil sands process-affected water

    Energy Technology Data Exchange (ETDEWEB)

    Kavanagh, Richard J., E-mail: rkavanag@uoguelph.ca [Department of Integrative Biology, University of Guelph, Guelph, ON, Canada N1G 2W1 (Canada); Frank, Richard A.; Solomon, Keith R. [Centre for Toxicology, University of Guelph, Guelph, ON, Canada N1G 2W1 (Canada); Van Der Kraak, Glen [Department of Integrative Biology, University of Guelph, Guelph, ON, Canada N1G 2W1 (Canada)

    2013-04-15

    Highlights: ► Fish were collected from a pond containing oil sands process-affected water (OSPW). ► They were compared to fish from two reference sites within the oil sands region. ► Differences in GSIs and tubercle numbers were observed in fish from the OSPW pond. ► Opercula, gills, and 11-KT concentrations also differed in fish from the OSPW pond. ► Black spot and tapeworms were not observed in any of the fish from the OSPW pond. -- Abstract: Previous laboratory based studies have shown that oil sands process-affected waters (OSPWs) containing high concentrations of naphthenic acids (>25 mg/l) have adverse effects on the reproductive physiology of fish. The purpose of this study was to assess the reproductive development and health of a wild population of fathead minnows (Pimephales promelas) inhabiting an OSPW pond that has moderate concentrations of naphthenic acids (∼10 mg/l). Fathead minnows were collected at various times during the period of 2006 through 2008 from Demonstration Pond (OSPW) located at Syncrude Canada Ltd., and two reference sites, Beaver Creek reservoir and Poplar Creek reservoir, which are all north of Fort McMurray, AB, Canada. Condition factor, gill histopathology, gonadosomatic indices, liver somatic indices, male secondary sexual characteristics, and plasma sex steroids were examined. Depending on the time of year that fathead minnows were collected, there were differences in the condition factor, gonadosomatic indices, liver somatic indices, and secondary sexual characteristics of fathead minnows (in males) from Demonstration Pond when compared to the fathead minnows from the reference sites. In comparison to reference fish, lower concentrations of 11-ketotestosterone were measured in the plasma of male fathead minnows collected from Demonstration Pond in June 2006 and July 2007. Black spot disease and Ligula intestinalis were prevalent in fathead minnows from the reference sites but were not observed in fathead minnows

  1. Developmental methoxychlor exposure affects multiple reproductive parameters and ovarian folliculogenesis and gene expression in adult rats.

    Science.gov (United States)

    Armenti, AnnMarie E; Zama, Aparna Mahakali; Passantino, Lisa; Uzumcu, Mehmet

    2008-12-01

    Methoxychlor (MXC) is an organochlorine pesticide with estrogenic, anti-estrogenic, and anti-androgenic properties. To investigate whether transient developmental exposure to MXC could cause adult ovarian dysfunction, we exposed Fischer rats to 20 microg/kg/day (low dose; environmentally relevant dose) or 100 mg/kg/day (high dose) MXC between 19 days post coitum and postnatal day 7. Multiple reproductive parameters, serum hormone levels, and ovarian morphology and molecular markers were examined from prepubertal through adult stages. High dose MXC accelerated pubertal onset and first estrus, reduced litter size, and increased irregular cyclicity (P<0.05). MXC reduced superovulatory response to exogenous gonadotropins in prepubertal females (P<0.05). Rats exposed to high dose MXC had increasing irregular estrous cyclicity beginning at 4 months of age, with all animals showing abnormal cycles by 6 months. High dose MXC reduced serum progesterone, but increased luteinizing hormone (LH). Follicular composition analysis revealed an increase in the percentage of preantral and early antral follicles and a reduction in the percentage of corpora lutea in high dose MXC-treated ovaries (P<0.05). Immunohistochemical staining and quantification of the staining intensity showed that estrogen receptor beta was reduced by high dose MXC while anti-Mullerian hormone was upregulated by both low- and high dose MXC in preantral and early antral follicles (P<0.05). High dose MXC significantly reduced LH receptor expression in large antral follicles (P<0.01), and down-regulated cytochrome P450 side-chain cleavage. These results demonstrated that developmental MXC exposure results in reduced ovulation and fertility and premature aging, possibly by altering ovarian gene expression and folliculogenesis.

  2. Aboveground feeding by soybean aphid, Aphis glycines, affects soybean cyst nematode, Heterodera glycines, reproduction belowground.

    Directory of Open Access Journals (Sweden)

    Michael T McCarville

    Full Text Available Heterodera glycines is a cyst nematode that causes significant lost soybean yield in the U.S. Recent studies observed the aphid Aphis glycines and H. glycines interacting via their shared host, soybean, Glycine max. A greenhouse experiment was conducted to discern the effect of A. glycines feeding on H. glycines reproduction. An H. glycines-susceptible cultivar, Kenwood 94, and a resistant cultivar, Dekalb 27-52, were grown in H. glycines-infested soil for 30 and 60 d. Ten days after planting, plants were infested with either zero, five, or ten aphids. At 30 and 60 d, the number of H. glycines females and cysts (dead females and the number of eggs within were counted. In general, H. glycines were less abundant on the resistant than the susceptible cultivar, and H. glycines abundance increased from 30 to 60 d. At 30 d, 33% more H. glycines females and eggs were produced on the resistant cultivar in the ten-aphid treatment compared to the zero-aphid treatment. However, at 30 d the susceptible cultivar had 50% fewer H. glycines females and eggs when infested with ten aphids. At 60 d, numbers of H. glycines females and cysts and numbers of eggs on the resistant cultivar were unaffected by A. glycines feeding, while numbers of both were decreased by A. glycines on the susceptible cultivar. These results indicate that A. glycines feeding improves the quality of soybean as a host for H. glycines, but at higher herbivore population densities, this effect is offset by a decrease in resource quantity.

  3. Impacts of reproductive technologies on beef production in the United States.

    Science.gov (United States)

    Dahlen, Carl; Larson, Jamie; Lamb, G Cliff

    2014-01-01

    Estimations of world population growth indicate that by the year 2050 we will reach nine billion habitants on earth. These estimates impose a tremendous challenge in the current agricultural systems as food supply will need to increase by 100 % in the next 40 years (Food and Agriculture Organization of the United Nations 2009). Beef will be a primary protein source that will assist in meeting the requirements for a portion of the protein in diets of this expanding global populace. Beef is a high-quality protein that contains all essential amino acids for the human body and also contains additional essential nutrients such as iron, zinc, B vitamins, riboflavin, selenium, choline, and conjugated linoleic acid (CLA). Adopting reproductive technologies at greater rates than currently used is a viable method to dramatically enhance production efficiency of beef cattle enterprises.Artificial insemination (AI), estrous synchronization and fixed-time AI (TAI), semen and embryo cryopreservation, multiple ovulation and embryo transfer (MOET), in vitro fertilization, sex determination of sperm or embryos, and nuclear transfer are technologies that are used to enhance the production efficiency of beef operations. In many cases, the development of these technologies is responsible for significant changes to traditional livestock production practices. However, adoption of these technologies appears to has not grown at the same rate in the United States as other formidable beef producing nations. For example, sales of beef semen for AI increased from 3.3 to 11.9 million units between 1993 and 2011 in Brazil, whereas that in the United States has increased from 2.9 to 3.8 million units during the same period. The significant increases in adoption of reproductive technologies in developing countries is likely as a result of the development of practical estrous synchronization and TAI systems that have allowed beef producers the opportunity to eliminate detection of estrus in their

  4. Asexual Reproduction of Phytophthora capsici as Affected by Extracts from Agricultural and Nonagricultural Soils.

    Science.gov (United States)

    Sanogo, S

    2007-07-01

    ABSTRACT Formation of sporangia and zoospores in species of Phytophthora is known to be influenced by soil microbial and chemical composition. In Phytophthora capsici, the study of the relationship of soil chemical composition to production of sporangia and zoospores has been limited. P. capsici is a soilborne pathogen of a wide array of vegetable crops, including chile pepper (Capsicum annuum) in New Mexico. Production of sporangia and zoospores by P. capsici was evaluated in extracts of soils from three different environments in New Mexico: (i) agricultural environments with a long history of chile pepper cropping and occurrence of P. capsici (CP), (ii) agricultural environments with no history of chile pepper cropping and no occurrence of P. capsici (Non-CP), and (iii) nonagricultural environments consisting of forests and rangelands (Non-Ag). There was a significant difference in production of P. capsici asexual propagules, expressed as natural log (number of sporangia x number of zoospores), among the three environments (P = 0.0298). Production of propagules was 9 to 13% greater in Non-Ag than in CP or Non-CP environments. Stepwise multiple discriminant analysis and canonical discriminant analysis identified the edaphic variables Na, pH, P, organic matter content, and asexual propagule production as contributing the most to the separation of the three environments. Two significant (P < 0.0001) canonical discriminant functions were derived with the first function, accounting for approximately 75% of the explained variance. Based on the two discriminant functions, approximately 93, 86, and 89% of observations in CP, Non-CP, and Non-Ag environments, respectively, were classified correctly. Soils from agricultural and nonagricultural environments differentially influence production of sporangia and zoospores in P. capsici, and soil samples could be effectively classified into agricultural and nonagricultural environments based on soil chemical properties and the

  5. Gender differences in Salix myrsinifolia at the pre-reproductive stage are little affected by simulated climatic change

    Energy Technology Data Exchange (ETDEWEB)

    Nybakken, L.; Julkunen-Tiitto, R. [Univ. of Eastern Finland. Dept. of Biology, Joensuu (Finland)

    2013-04-15

    Females of dioecious species are known often to prioritize defense, while males grow faster. As climatic change is known to influence both growth and defense in plants, it would be important to know whether it affects the sexes of dioecious species differently. This could have impacts on future sex ratios in nature. We grew four clones of each sex of Salix myrsinifolia in greenhouse chambers under ambient conditions, enhanced temperature, enhanced CO{sub 2} or enhanced temperature? + enhanced CO{sub 2}. The females had the greatest growth and also the highest levels of phenolic compounds in twigs, while in leaves some compounds were higher in males, some in females. Enhanced CO{sub 2} increased growth equally in both sexes, while growth was not affected by elevated temperature. Phenolic compounds in twigs were, however, lowered under elevated temperature. The gender differences were not strongly affected by the simulated climatic changes, but the effects seen on some highly concentrated compounds may be important. We interpret the intensive growth at pre-reproductive phase as a strategy in females to get an initial advantage before later periods with fewer resources available for growth. (Author)

  6. Gender differences in Salix myrsinifolia at the pre-reproductive stage are little affected by simulated climatic change.

    Science.gov (United States)

    Nybakken, Line; Julkunen-Tiitto, Riitta

    2013-04-01

    Females of dioecious species are known often to prioritize defense, while males grow faster. As climatic change is known to influence both growth and defense in plants, it would be important to know whether it affects the sexes of dioecious species differently. This could have impacts on future sex ratios in nature. We grew four clones of each sex of Salix myrsinifolia in greenhouse chambers under ambient conditions, enhanced temperature, enhanced CO2 or enhanced temperature  +  enhanced CO2 . The females had the greatest growth and also the highest levels of phenolic compounds in twigs, while in leaves some compounds were higher in males, some in females. Enhanced CO2 increased growth equally in both sexes, while growth was not affected by elevated temperature. Phenolic compounds in twigs were, however, lowered under elevated temperature. The gender differences were not strongly affected by the simulated climatic changes, but the effects seen on some highly concentrated compounds may be important. We interpret the intensive growth at pre-reproductive phase as a strategy in females to get an initial advantage before later periods with fewer resources available for growth.

  7. The affecting of perceived product quality and perceived risk on perceived product value

    OpenAIRE

    Pisnik Korda, Aleksandra

    2015-01-01

    Perceived value is an extremely important concept in marketing and many authors have dealt with it in the recent years. There are numerous factors affecting perceived product value and we focused on three: perceived product price, perceived product quality and perceived risk. In this paper we present two of them: perceived product quality and perceived risk. Perceived product value is the difference between value in use and price. Value in use is a mix of benefits, which a customer is likely ...

  8. Reproduction of Meloidogyne incognita on Winter Cover Crops Used in Cotton Production.

    Science.gov (United States)

    Timper, Patricia; Davis, Richard F; Tillman, P Glynn

    2006-03-01

    Substantial reproduction of Meloidogyne incognita on winter cover crops may lead to damaging populations in a subsequent cotton (Gossypium hirsutum) crop. The amount of population increase during the winter depends on soil temperature and the host status of the cover crop. Our objectives were to quantify M. incognita race 3 reproduction on rye (Secale cereale) and several leguminous cover crops and to determine if these cover crops increase population densities of M. incognita and subsequent damage to cotton. The cover crops tested were 'Bigbee' berseem clover (Trifolium alexandrinum), 'Paradana' balansa clover (T. balansae), 'AU Sunrise' and 'Dixie' crimson clover (T. incarnatum), 'Cherokee' red clover (T. pratense), common and 'AU Early Cover' hairy vetch (Vicia villosa), 'Cahaba White' vetch (V. sativa), and 'Wrens Abruzzi' rye. In the greenhouse tests, egg production was greatest on berseem clover, Dixie crimson clover, AU Early Cover hairy vetch, and common hairy vetch; intermediate on Balansa clover and AU Sunrise crimson clover; and least on rye, Cahaba White vetch, and Cherokee red clover. In both 2002 and 2003 field tests, enough heat units were accumulated between 1 January and 20 May for the nematode to complete two generations. Both AU Early Cover and common hairy vetch led to greater root galling than fallow in the subsequent cotton crop; they also supported high reproduction of M. incognita in the greenhouse. Rye and Cahaba White vetch did not increase root galling on cotton and were relatively poor hosts for M. incognita. Only those legumes that increased populations of M. incognita reduced cotton yield. In the southern US, M. incognita can complete one to two generations on a susceptible winter cover crop, so cover crops that support high nematode reproduction may lead to damage and yield losses in the following cotton crop. Planting rye or Meloidogyne-resistant legumes as winter cover crops will lower the risk of increased nematode populations

  9. Nanoplastic affects growth of S. obliquus and reproduction of D. magna

    NARCIS (Netherlands)

    Besseling, E.; Wang, B.; Lürling, M.F.L.L.W.; Koelmans, A.A.

    2014-01-01

    The amount of nano- and microplastic in the aquatic environment rises due to the industrial production of plastic and the degradation of plastic into smaller particles. Concerns have been raised about their incorporation into food webs. Little is known about the fate and effects of nanoplastic, espe

  10. Rooting with neighbours : Detection affects growth and reproductive strategies of plants

    NARCIS (Netherlands)

    Chen, B.

    2015-01-01

    Evolutionary game-theoretical studies have indicated that plant populations with maximum seed production per unit area can be invaded by a mutant or intruder that grows more leaves, is taller or produces more roots, and that an evolutionarily stable vegetation is therefore less than maximally produc

  11. THE EFFECT OF BREED ON REPRODUCTIVE PERFORMANCE IN COMMODITY RABBIT PRODUCTION

    Directory of Open Access Journals (Sweden)

    Jadwiga TOPCZEWSKA

    2013-06-01

    Full Text Available The aim of this study was to assess the reproductive rates among different breeds, mostly used in commodity farm conditions. The studies were obtained at fertility level from 6.27 animals in the Californian breed to 8.49 animals in Popielno White. High rate of failures during rearing was observed in the Californian breed. Litter weight was dependent on the number of births and young rearing, lactation and care of female. Kitten weight at 35 day of age ranged from 603.21g (Alaskan to 736.10g (Giant Chinchilla. The results indicate the usefulness of a Popielno White breed for the livestock production.

  12. Twinning in Iranian Holstein Dairy Cattle: A Study of Risk Factors and Production and Reproduction Consequences

    Directory of Open Access Journals (Sweden)

    abolfazl mahnani

    2016-08-01

    Full Text Available Introduction Cattle are a monotocous species meaning that, under most circumstances, a successful pregnancy results in the birth of one calf. Twinning rate has been reported in dairy cows from 3 to 5 percent, which can be influenced by maternal age.The birth of twins is detrimental to the majority of beef and dairy cattle producer. Financial loss arising from any of twinning has been reported in Europe between 109 to 201 dollars in recent years. Because it is associated with undesirable consequences such as reduced survival, calf, cow increased removal rate and poor performance. This also reduces pregnancy rates and profitability herds. One of the effects of twinning severe is reduction of the number of calves for replacement fertility in dairy cows. This is a loss arising from an increase in infant mortality and a gender bias in bull calves homo zygote.Twinning rate increases significantly the incidence of reproductive abnormalities, including the retained placenta, dystocia, stillbirth and abortion. Many studies have been done on the effect of multiple pregnancies in cattle production and reproduction. Higher milk production for cows twin issue is controversial as some studies have shown that there is a positive correlation between the rate of twinning in dairy cattle and milk production. But in the next lactation, production for cows that have been the twin of the infected cow metabolic disease in the previous period was lower. In a study reported that cows spend fewer days in the twin peak production. The results of the study on the effect of twinning on reproductive traits of Holstein cows-Farzin showed that only half of the twin cows are prone to reproduce in the next period. It is also reported a greater number of insemination per conception in twin compared to single cows. In addition, it has been reported that the twin was more than 15 days from calving to first services. Average twin cows experiencing 1.7 times more death and removal

  13. Factors affecting the dairy industry's products export in Iran

    Directory of Open Access Journals (Sweden)

    Aazam Yazdaninasab

    2016-09-01

    Full Text Available Export dairy products as an important part of non-oil exports can play an important role in improving the economic situation of Iran. Therefore, in this study factors affecting the exports of dairy products in the period 2002-2014 will be discussed. The results indicate a trend of increasing exports of dairy products during the 13 year study. This reflects the fact that the dairy industry of great potential capacity to provide part of the non-oil revenues in the agricultural subsector. In this study, the dependent variable was the amount of exports of dairy products. Independent variables included: investment in large dairy factories, the price of goods and services consumed by this sector, GDP, exchange rate, export prices for dairy products. The results showed that the effect of all independent variables on the dependent variable is positive and rising: such as investment and exchange rate. So that with an increase of 10 percent each of the indicators the country's exports of dairy products will be increase. The original proposal of the present study is: the use of policies and financial instruments and non-financial, such as paying attention to the comparative advantages of export, focusing on the right target export markets in order to support the country's dairy industry to increase production and exports.

  14. Reproductive efficiency and metabolism of female broiler breeders as affected by genotype, feed allocation, and age at photostimulation. 3. Reproductive efficiency.

    Science.gov (United States)

    Zuidhof, M J; Renema, R A; Robinson, F E

    2007-10-01

    A 3 x 4 x 2 factorial trial was performed to test the effect of 3 broiler breeder strains, 4 target BW profiles, and 2 photostimulation ages on egg and chick production. Hubbard Hi-Y, Ross 508, and Ross 708 pullets were reared on BW profiles that separated at 4 wk and converged at 32 wk: standard (approximate mean target BW profile of the 3 strains used), low (12-wk BW target = 25% lower than standard followed by rapid gain to 32 wk), moderate (12-wk BW target = 150% of standard followed by lower rate of gain to 32 wk), and high (12-wk BW target = 200% of standard followed by minimal growth to 32 wk). Birds were photostimulated at 18 (18WK) or 22 wk (22WK). Twelve birds per interaction (n = 288) were individually caged at 17 wk and monitored for egg and chick production to 58 wk of age. The low BW profile delayed onset of lay, particularly in 18WK hens. Low and standard birds had a greater initial egg weight than moderate birds. The 18WK photostimulation age resulted in production of 31 small eggs (birds. A settable (52 g) egg weight was reached earliest by standard birds (187 d of age). Average prime sequence length was reduced by 7.0 eggs in moderate and high birds compared with other profiles. The 18WK hens laid 9 more eggs (174) than 22WK birds, with no effect on unsettable eggs. The 22WK birds laid 7 more settable eggs due to increased early egg size. Total egg output was similar among BW profile treatments, although Ross 708-high hens under-performed compared with the other 708 profiles. The Ross 508-high birds laid the same number of eggs as Ross 508-standard birds (mean = 178.7). Feeding profiles affected egg production traits differently among strains. Feed intake had more effect on egg size and early production traits than BW profile or BW.

  15. Appropriate timing of uterine cavity length measurement positively affects assisted reproduction cycle outcome.

    Science.gov (United States)

    Madani, Tahereh; Ashrafi, Mahnaz; Abadi, Akram Bahman; Kiani, Kiandokht

    2009-11-01

    An appropriate and easy embryo transfer has a direct impact on pregnancy rates. Proper evaluation of the uterocervical axis and uterine depth are necessary for suitable embryo transfer. The aim of this study was to assess the appropriate time for cervical axis evaluation and uterine measurement. A total of 124 patients undergoing IVF treatment were included in the study. They were divided equally into two groups. In group I (62 women), uterine cavity depth was measured and the uterocervical axis was determined on day 2 or 3 of the menstrual cycle, and in group II (62 women) at the time of oocyte retrieval. There was a statistically significant difference in clinical pregnancy rates between the two groups (P = 0.006). Thirty-four women became pregnant in group I (64.2%) versus 19 women in group II (35.8%). In conclusion, uterine cavity measurement is necessary for suitable embryo transfer. It seems that the time of measurement significantly affects clinical pregnancy rate in IVF cycles. The best time for uterine measurement is on day 2 or 3 of menstruation.

  16. Individual heterogeneity and offspring sex affect the growth-reproduction trade-off in a mammal with indeterminate growth.

    Science.gov (United States)

    Gélin, Uriel; Wilson, Michelle E; Cripps, Jemma; Coulson, Graeme; Festa-Bianchet, Marco

    2016-04-01

    Reproduction can lead to a trade-off with growth, particularly when individuals reproduce before completing body growth. Kangaroos have indeterminate growth and may always face this trade-off. We combined an experimental manipulation of reproductive effort and multi-year monitoring of a large sample size of marked individuals in two populations of eastern grey kangaroos to test the predictions (1) that reproduction decreases skeletal growth and mass gain and (2) that mass loss leads to reproductive failure. We also tested if sex-allocation strategies influenced these trade-offs. Experimental reproductive suppression revealed negative effects of reproduction on mass gain and leg growth from 1 year to the next. Unmanipulated females, however, showed a positive correlation between number of days lactating and leg growth over periods of 2 years and longer, suggesting that over the long term, reproductive costs were masked by individual heterogeneity in resource acquisition. Mass gain was necessary for reproductive success the subsequent year. Although mothers of daughters generally lost more mass than females nursing sons, mothers in poor condition experienced greater mass gain and arm growth if they had daughters than if they had sons. The strong links between individual mass changes and reproduction suggest that reproductive tactics are strongly resource-dependent.

  17. Differences in productive robustness in rabbits selected for reproductive longevity or litter size

    DEFF Research Database (Denmark)

    Theilgaard, R; Baselga, M; Blas, E

    2009-01-01

    The aim of this work was to evaluate the ability of a line selected for reproductive longevity (LP) to confront productive challenges compared to a line selected during 31 generations for litter size at weaning (V). A total of 133 reproductive rabbit does were used (72 and 61 from LP and V lines......, respectively). Within each line, three groups with different levels of productive effort were planned: PP9, inseminated at day 4 after the first partum and with nine kits during the second partum, and inseminated after first weaning (30 days) and with nine (PW9) or five kits (PW5) during the second partum...... lines during the first week when litter size was nine (60 v. 54 g of milk/kg LW0.75 day for LP and V does, respectively; P,0.01), but no difference when litter size was five. Consequently, when litter size was nine, LP does showed a lower recovery of PFT (0.6mm less; P,0.05) than V does during the first...

  18. Energy sources in low intake supplements on the productive and reproductive performance of Zebu cows

    Directory of Open Access Journals (Sweden)

    Marcelo Marcondes de Godoy

    2015-06-01

    Full Text Available The aim of this study was to evaluate the productive and reproductive performance of primiparous zebu cows supplemented on Brachiaria brizantha cv. Marandu with supplements of low intake composed of different energy sources in the postpartum period. Sixty cow-calf pairs were divided into three treatments, a standard mineral salt supplement, a supplement based on ground corn and another containing protected fat, under the same conditions of pasture. The cows had an average initial age of 36 months and 295.9 ± 20 kg of initial body weight. The evaluation period lasted from November 2006 to May 2007, the 24 days after delivery until weaning of calves to 192 days of age. Cows fed the supplement based on ground corn (351 g day-1 and protected fat (357 g day-1 showed a similar increase in body weight, which was higher in relation cows fed with the mineral mixture (179 g day-1. Mineral mixture supplementation resulted in body condition score loss of cows between 80 and 136 days after calving. Cows fed energy supplements of low consumption produced more milk, weaned heavier calves and showed higher pregnancy rate than those fed only with the mineral mixture. The use of 3% calcium salts of fatty acids in energy supplements of low consumption did not alter the productive and reproductive performance of primiparous Zebu cows.

  19. Production of reproductively sterile fish: A mini-review of germ cell elimination technologies.

    Science.gov (United States)

    Wong, Ten-Tsao; Zohar, Yonathan

    2015-09-15

    As seafood consumption shifts from fisheries harvests to artificially propagated aquatic species, the increase of aquaculture activities poses a biological threat to our environment. Selectively bred, non-native and (eventually) genetically engineered farmed fish may escape from aquaculture operations, propagate and/or interbreed with wild stocks and subsequently alter the genetic makeup of populations in the environment. Thus, an effective strategy for bio-containment of farmed fish is critically needed. Farming reproductively sterile fish is the most environmentally sustainable approach to ensure complete bio-containment in large-scale aquaculture operations. Chromosome set manipulations to produce sterile fish, including polyploidy and hybridization, are currently the most common practices in the aquaculture industry. However, they do not always result in 100% sterility of the treated fish. Moreover, triploid fish typically do not perform as well as the non-manipulated diploids under commercial culture conditions. In the last half decade, several genetic engineering methods have been developed to produce sterile fish. In this review, we will address the latest technologies that use transgenic approaches to eliminate germ cells, resulting in the production of sterile fish. These latest advances also led us to the development of egg/embryo immersion methodologies to deliver and screen compounds that can be used to eliminate primordial germ cells and produce sterile fish. This emerging non-transgenic strategy for the production of reproductively sterile fish in aquaculture will also be discussed.

  20. Epidemiological studies of reproductive performance indicators in Swedish dairy cows

    OpenAIRE

    Löf, Emma

    2012-01-01

    Reproductive efficiency in dairy cows is a key factor for milk producers, and numerous studies have identified impaired reproductive performance as a major cause of reduced production efficiency in the dairy industry. The overall aim of this thesis was to gain knowledge of factors affecting the reproductive performance indicators currently used by herd advisory services and to find other, possibly more efficient, ways to measure reproductive performance in dairy cows. The studies include...

  1. The effects of bovine necrotic vulvo-vaginitis on reproductive and production performance of Israeli 1st calf heifers.

    Science.gov (United States)

    Goshen, T; Ben-Gera, J; Koren, O; Bdolah-Abram, T; Elad, D

    2012-04-01

    Bovine necrotic vulvovaginitis (BNVV) is a syndrome unique to Israel characterized by necrotic lesion in the caudal vagina mainly in first calf heifers after calving, associated with Porphyromonas levii. The objectives of this study were to analyze the impact of BNVV on reproductive performance, milk production and survival in the heard of first calf dairy heifers in affected farms, and to verify if the effects of BNVV are severity-dependent. For assessment of the severity level a scale of 4 degrees was formed, and cows were scored 4 to 6 d after calving. Data were obtained from two dairy farms during 2006-07, consisting of 603 lactations. The incidence and the severity of BNVV declined between 2006 and 2007, and severe BNVV tended to be more prevalent in the summer. The odds to conceive in the first artificial insemination of BNVV cow tended to be lower than healthy cows (OR = 0.676, P = 0.052). Cows with BNVV had longer empty period (145.8 d vs. 135.1 d of healthy cows, P = 0.031), but only severe BNVV had a negative effect on the odds of the cow to be empty at 150 d in milk (DIM) (OR = 2.05, P = 0.052). Severe BNVV also affected the mean survival time to conception (155.9 d vs. 142.3 d, P = 0.042). All BNVV severity degrees had a negative effect on milk production. The effect on milk production was not limited only to the beginning of the lactation, cows with BNVV produced 338.1 kg milk less than healthy cows (P = 0.016) in 305 d corrected lactation. The effect on milk production was not severity depended. No effect on survival time in the herd was demonstrated.

  2. Preliminary study on some productive and reproductive traits of Saanen goats under Sudan conditions

    Directory of Open Access Journals (Sweden)

    Tag Eldin sabil,

    2011-02-01

    Full Text Available The research aimed to investigate the difference in performance of pure Saanen goats reared in Sudan and Swiss the origin of the animal considering birth weight, twining, puberty, mating age, gestation period, motility rate, milk production and quality. 200 does from Goat Research Centre (GRC at Khartoum were used for the study for three consecutive years (2008-2010. The results show that hot climate (Sudan delayed the reproductive parameters of the goat and increased the mortality rate. On the other hand milk production decreased but no change in the quality was observed. The research concluded that keeping pure Saanen in Sudan is not beneficial and it is recommended to cross the animal with local breeds. Moreover, improve the environment for better performance and welfare of the animals.

  3. Estrogenic octylphenol affects seminal fluid production and its biochemical composition of eelpout (Zoarces viviparus).

    Science.gov (United States)

    Rasmussen, Tina H; Korsgaard, Bodil

    2004-10-01

    Estrogenic chemicals such as alkylphenols (APs) have been shown to disrupt the reproductive system of male fish. In the present study, the effects of the estrogenic chemical octylphenol (100 microg g(-1)) and 17 beta-estradiol on the semen production and the biochemical composition of the seminal fluid of the viviparous eelpout (Zoarces viviparus) were investigated at the time of spawning. After 10 days of octylphenol or estrogen treatment, vitellogenin (Vtg) synthesis was induced as indicated by increased plasma vitellogenin concentration. In accordance with the increased vitellogenin concentration, hepatosomatic index (HSI), total protein concentration, and total calcium concentration were also increased, and free amino acids concentration was decreased in blood plasma. Octylphenol treatment caused a decrease in the gonadosomatic index (GSI) and the milt volume and an increase in the spermatocrit. The histological examination revealed that octylphenol affected the normal lobular structure, including the Sertoli cells. In the majority of the octylphenol-treated fish, trapped sperm cells were observed in parts of the seminiferous lobules and the sperm ducts. The biochemical composition of the seminal fluid was also affected by the octylphenol or estrogen. The seminal plasma concentrations of magnesium, calcium, and total protein were elevated, and the concentration of free amino acids was reduced in the treated fish. This study indicates that octylphenol inhibits the seminal fluid production and changes its biochemical composition in eelpouts.

  4. Do ICTs Affect Workforce Productivity in Egyptian Industrial Organizations?

    Directory of Open Access Journals (Sweden)

    M. Elsaadani

    2014-06-01

    Full Text Available The present study aims to investigate the influence of Information Communication Technologies-ICTs’ dimensions (Information Technology (IT, Management Information System (MIS, Office automation (OA, Intranet and Internet on workforce productivity for a group of industrial organizations in Alexandria - Egypt. The population of the study included managers and staff members working in different areas related to ICTs in selected industrial organizations at various managerial levels. A descriptive-statistical combined research study was conducted. Simple random sampling was used for the selection of the participating industrial organization. A questionnaire was used as the data collection method. Expert comments were used to check the validity of study instrument, and the reliability of questions was calculated as 79% using Cronbach’s Alpha coefficient. Single variable t-test, Friedman and variance analysis tests were used for the analysis. Study findings revealed that the specified dimensions of ICTs positively affect workforce productivity of industrial organizations in Alexandria - Egypt.

  5. Effect of neosporosis on productive and reproductive performance of dairy cattle in Costa Rica.

    Science.gov (United States)

    Romero, J J; Breda, S Van; Vargas, B; Dolz, G; Frankena, K

    2005-12-01

    A cross-sectional study was conducted to assess the effect of neosporosis on productive and reproductive parameters in dairy cows. Cows (n=2743) from 94 farms located in the most important dairy areas in Costa Rica were used in the study. The size of the herds ranged from 32 to 379 females (mean=110, median=125). An indirect ELISA was used to determine the serostatus of the cows towards Neospora caninum. The effect of neosporosis on milk production was analysed by a mixed linear model. In addition, the effects on calving interval (days) and calving to conception interval (days) were analysed by survival analysis. The risk of abortion in relation to N. caninum serostatus was assessed by logistic regression, with herd as a random effect. Overall, 1185 of 2743 cows (43.3%) were seropositive for Neospora. Eighty-nine of 94 (94.7%) farms were classified as Neospora-seropositive. It was estimated that cows seronegative to Neospora produced an additional 84.7L of milk (P=0.6). Serostatus did not have a significant effect on the length of the calving interval in the Cox proportional hazard survival analysis (Hazard ratio=1.2, 95% CI: 0.9, 1.4). The logistic regression model had a weak positive association between serostatus and abortion (OR 1.7, 95% CI: 0.8, 3.9), but did not show a strong association between serostatus and the number of services per conception (OR=0.95, 95% CI: 0.7, 1.3). In conclusion, there were no significant effects of Neospora serostatus on production and reproductive performance in this study.

  6. Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

    Institute of Scientific and Technical Information of China (English)

    LI Wen-gui; DAI Fei-yan; CHENG Yong-xian; YIN Ge-fen; BI Jun-long; LI Dian-peng

    2013-01-01

    Porcine reproductive and respiratory syndrome(PRRS) caused by porcine reproductive and respiratory syndrome virus(PRRSV) is one of the most infectious diseases in the swine industry worldwide,causing big economic losses.Vaccines are major weapons against PRRSV,however,current available vaccines have several limitations.Developing chemical drugs as alternatives is required.On the basis of traditional medical knowledge,we purposely selected 15 natural products originated from Chinese herbs with anti-infectious effects.Their antiviral activities were evaluated by PRRSV-induced cytopathic effect(CPE) on MARC-145 cells and reverse transcription polymerase chain reaction(RT-PCR) assay.Compounds ethoxysanguinarine(EOSG) and atractylodinol were found to be the hits which could significantly reduce PRRSV-associated CPE with 50% inhibited concentration(IC50) values of 7.9 and 39.4 μmol/L,respectively.Meanwhile,compounds ethoxysanguinarine and atractylodinol significantly decreased mRNA expression of ORF7 gene in a dose-dependent manner.Study results suggest that compounds ethoxysanguinarine and atractylodinol may be useful anti-PRRSV drugs for swine industry or the hits for further lead optimization.

  7. Grape variety affects larval performance and also female reproductive performance of the European grapevine moth Lobesia botrana (Lepidoptera: Tortricidae).

    Science.gov (United States)

    Moreau, J; Benrey, B; Thiéry, D

    2006-04-01

    For insect herbivores, the quality of the larval host plant is a key determinant of fitness. Therefore, insect populations are supposed to be positively correlated with the nutritional quality of their host plant. This study aimed to determine if and how different varieties of grapes (including the wild grape Lambrusque) affect both larval and adult performance of the polyphagous European grapevine moth Lobesia botrana (Denis & Schiffermüller). Significant differences were found in larval development time, but not in pupal mass, adult emergence rate, or sex ratio. Although the fecundity of females is not different among varieties, females fed on some varieties produced eggs of different sizes which are correlated to their fertility. Thus, females adapt resource allocation to eggs depending on their diet as larvae. Using a fitness index, the average reproductive output was found to be highest for females reared on cv. Chardonnay. Females reared on wild grape produced a fitness index identical to the cultivated grapes. However, Lambrusque and Gewurztraminer separate themselves from the cultivated varieties according to our discriminant analyses. It is emphasized, through this study, that cultivars fed on by larvae should be considered in the population dynamics of L. botrana and that egg number is insufficient to determine host plant quality.

  8. Factors affecting reproductive performance of white-tailed deer subjected to fixed-time artificial insemination or natural mating.

    Science.gov (United States)

    Mellado, Miguel; Orta, Claudia G; Lozano, Eloy A; García, Jose E; Veliz, Francisco G; de Santiago, Angeles

    2013-01-01

    The objectives of this study were to examine the effects of several factors affecting fawning rate, litter size, litter weight and neonatal fawn mortality in white-tailed deer inseminated either transcervically or by means of laparoscopy. Oestrus synchronisation with a controlled internal drug release (CIDR)-based protocol and fixed-time artificial insemination (FTAI) was conducted in 130 white-tailed deer (Odocoileus virginianus texanus) during three reproductive seasons (2007-2009; 271 services) in a game-hunting ranch in a hot-arid environment (26°4' N, 101°25' W). Ninety additional non-treated does were exposed to bucks for natural mating. Fawning rate did not differ between AI methods (40.0 vs 45.0% for transcervical and laparoscopic AI, respectively). Overall fawning rate (proportion of all does fawning after FTAI and a subsequent period of buck exposure) did not differ between transcervical (89.5%), laparoscopic (80.3%) or natural (88.9%) insemination. Litter size per fawning doe was higher (Pinseminated does (1.40±0.51) or in laparoscopically-inseminated does (1.48±0.50). The main conclusion was that no enhancement of fawning rate or litter size occurred as a result of intrauterine deposition of semen by laparoscopy compared with the transcervical insemination technique.

  9. Developmental exposure to ethinylestradiol affects reproductive physiology, the GnRH neuroendocrine network and behaviors in female mouse

    Directory of Open Access Journals (Sweden)

    Lyes eDerouiche

    2015-12-01

    Full Text Available During development, environmental estrogens are able to induce an estrogen mimetic action that may interfere with endocrine and neuroendocrine systems. The present study investigated the effects on the reproductive function in female mice following developmental exposure to pharmaceutical ethinylestradiol (EE2, the most widespread and potent synthetic steroid present in aquatic environments. EE2 was administrated in drinking water at environmentally relevant (ENVIR or pharmacological (PHARMACO doses (0.1 and 1 µg/kg (body weight/day respectively, from embryonic day 10 until postnatal day 40. Our results show that both groups of EE2-exposed females had advanced vaginal opening and shorter estrus cycles, but a normal fertility rate compared to CONTROL females. The hypothalamic population of GnRH neurons was affected by EE2 exposure with a significant increase in the number of perikarya in the preoptic area of the PHARMACO group and a modification in their distribution in the ENVIR group, both associated with a marked decrease in GnRH fibers immunoreactivity in the median eminence. In EE2-exposed females, behavioral tests highlighted a disturbed maternal behavior, a higher lordosis response, a lack of discrimination between gonad-intact and castrated males in sexually experienced females, and an increased anxiety-related behavior. Altogether, these results put emphasis on the high sensitivity of sexually dimorphic behaviors and neuroendocrine circuits to disruptive effects of EDCs.

  10. Bacillus thuringiensis insecticidal crystal proteins affect lifespan and reproductive performance of Helicoverpa armigera and Spodoptera exigua adults.

    Science.gov (United States)

    Zhang, Ying; Ma, Yan; Wan, Pin-Jun; Mu, Li-Li; Li, Guo-Qing

    2013-04-01

    Being delivered as sprays or expressed in plant, Bacillus thuringiensis (Bt) crystalline proteins (Cry toxins) display insecticidal activities against numerous Lepidopteran, Dipteran, and Coleopteran larvae. Comparative study of toxicities of Bt Cry toxins between larvae and adults may afford important new insights into the interactions of the toxins with receptor proteins in host insect, and represent intriguing targets for the control of insect pests. However, the effectiveness of Bt Cry toxins in insect adults has paid less attention. In the present article, the effectiveness of Cry1Ac and Cry1Ca on lifespans and reproductive performance of Helicoverpa armigera (Hübner) and Spodoptera exigua (Hübner) adults were evaluated by in vivo experiments. Considering transgenic plants express modified, truncated versions of cry genes yielding active toxin fragment, we used activated Bt toxins at the concentration of 500, 100, and 20 microg/ml in a 10% sucrose aquous solution. At the highest concentration, Cry1Ac and Cry1Ca shortened 48.1 and 48.9% of H. armigera female lifespan, and 43.5 and 38.5% of S. exigua female lifespan, and they reduced 37.8 and 40.3%, and 50.5 and 47.4% of H. armigera and S. exigua male lifespans respectively. Bt toxins negatively affected copulation. Exposure to 500 microg/ml of Cry1Ac and Cry1Ca greatly reduced 50.0 and 46.8%, and 58.7 and 57.3% spermatophore acceptance by H. armigera and S. exigua females, respectively. Similarly, Cry1Ac and Cry1Ca exposure decreased 40.0 and 50.3%, and 61.3 and 60.0% of spermatophore transfer by H. armigera and S. exigua males, respectively. Moreover, exposure females rather than males to 500 microg/ml of Cry1Ac and Cry1Ca significantly dropped 57.5 and 57.5% of the number of eggs laid by H. armigera, and 35.4 and 45.8% of the number of egg masses deposited by S. exigua. In contrast, both Cry1Ac and Cry1Ca did not negatively influence the egg hatchability. At the middle and the lowest concentrations, however

  11. Effect of calf sex on some productive, reproductive and health traits in Holstein cows

    Energy Technology Data Exchange (ETDEWEB)

    Chegini, A.; Hossein-Zadeh, N.G.; Hosseini-Moghadam, H.

    2015-07-01

    Records of Holstein cows from March 1992 to April 2008 from 194 large herds and comprising from 402,716 records for productive traits to 178,344 records of somatic cell count were used to study the effect of calf sex in different parities and calving season on the subsequent productive, reproductive and health traits in Holstein cows. T-test procedure of SAS software was used to investigate the effect of calf sex and season of calving on aforementioned traits. Cows with female calves had higher milk and fat yield, persistency of milk and fat yield and longer lactation length, while cows that gave birth to male calves had shorter calving interval and longer productive life. Also, cows with female calves had higher milk yield per day of lactation in the first two parities, but there was no difference in milk yield per day of lactation for parities ≥ 3. There was no relationship among mean somatic cell count and sex of born calf. Fall calves had the highest adjusted milk yield and milk yield per day of lactation, however, winter calves had the longest lactation lengthand productive life and the highest somatic cell count. Results from this study demonstrate that it seems necessary to consider the effect of calf sex on aforementioned traits when making decision to use sexed semen or conventional semen. (Author)

  12. Potato (Solanum tuberosum) greenhouse tuber production as an assay for asexual reproduction effects from herbicides.

    Science.gov (United States)

    Olszyk, David; Pfleeger, Thomas; Lee, E Henry; Plocher, Milton

    2010-01-01

    The present study determined whether young potato plants can be used as an assay to indicate potential effects of pesticides on asexual reproduction. Solanum tuberosum (Russet Burbank) plants were grown from seed pieces in a mineral soil in pots under greenhouse conditions. Plants were treated with herbicides (cloransulam, dicamba, glyphosate, imazapyr, primsulfuron, sulfometuron, or tribenuron) at simulated drift levels [Production of small tubers and shoot dry weight were determined at approximately 28 DAT. Imazapyr, sulfometuron, and tribenuron caused significant reductions in tuber fresh weight, with the effective concentrations producing a 25% potato tuber fresh weight (EC25) of 0.00038, 0.0016, and 0.0021 x f.a.r. of 1,124, 52, and 9 g active ingredient hectare(-1) (g a.i. HA(-1)), respectively. Primisulfuron, dicamba, and cloransulam also significantly reduced tuber fresh weight, but with higher EC25 values of 0.011, 0.07, and 0.010 to 0.2 x f.a.r. of 40, 558, and 18 g a.i. HA(-1), respectively. Glyphosate had little effect on tuber fresh weight, with a significant reduction in only one experiment. Sulfometuron reduced tuber fresh weight at an EC25 value lower than the EC25 values for shoot dry weight or plant height. For other herbicides, the reduction in tuber fresh weight occurred within the range of EC25 values for other responses. Although additional experiments are required to develop further a phytotoxicity test, these results indicated that tuber production in young potato plants (harvested approximately 42 DAE) may be an effective assay for below-ground asexual reproductive responses to herbicides, especially acetolactate synthase inhibitors.

  13. Does catch and release affect the mating system and individual reproductive success of wild Atlantic salmon (Salmo salar L.)?

    Science.gov (United States)

    Richard, Antoine; Dionne, Mélanie; Wang, Jinliang; Bernatchez, Louis

    2013-01-01

    In this study, we documented the breeding system of a wild population of Atlantic salmon (Salmo salar L.) by genetically sampling every returning adult and assessed the determinants of individual fitness. We then quantified the impacts of catch and release (C&R) on mating and reproductive success. Both sexes showed high variance in individual reproductive success, and the estimated standardized variance was higher for males (2.86) than for females (0.73). We found a weak positive relationship between body size and fitness and observed that fitness was positively correlated with the number of mates, especially in males. Mature male parr sired 44% of the analysed offspring. The impact of C&R on the number of offspring was size dependent, as the reproductive success of larger fish was more impaired than smaller ones. Also, there was an interactive negative effect of water temperature and air exposure time on reproductive success of C&R salmon. This study improves our understanding of the complex reproductive biology of the Atlantic salmon and is the first to investigate the impact of C&R on reproductive success. Our study expands the management toolbox of appropriate C&R practices that promote conservation of salmon populations and limit negative impacts on mating and reproductive success.

  14. Production systems and reproductive performances of Camelus dromedarius in Somali regional state, eastern Ethiopia

    Directory of Open Access Journals (Sweden)

    Simenew Keskes

    2013-12-01

    Full Text Available Across-sectional questionnaire survey and focused group discussions were conducted to characterize camel production systems and to evaluate reproductive performances of camels at their natural pastoralist management systems of Somali region. A total of 100 households were included in the study during the period of October 2012 to March 2013. About 98% of Somali pastoralists preferred camels as their first choice over other livestock species and mainly kept in the society for milk and meat production. The camel management dominating in the study areas of Somali region is traditional nomadic. Camel is one of the most important livestock for Somali pastoralists’ livelihood as a source of milk, meat and draught power. Mature female camels were dominant (54.87% in the camel herd. The ratio of male to female camel was 1:13. Mean age at first calving and calving interval were 62.16±10.44 and 23.28±3.36 months respectively. Age at first calving and calving interval can be minimized to 57±5.52 and 21.84±4.8 months by proper husbandry and health care. The mean lactation length was 11.51±1.91 months. Diseases and predators were reported as the main causes of calf mortality. In the herd dynamic simulation calf mortality rate can be reduced at least to 7% only by preventing predators attack. Diseases (66%, lack of pasture (59% and security (47% were the main constraints in camel production of the study areas. For the better productivity of camels, the major constraints such as disease problems, lack of pasture and tribal conflicts should be mitigated. Proper husbandry and health services can play significant roles in the long term improvement of camel production and productivity of the region.  

  15. Knowledge Elecitation for Factors Affecting Taskforce Productivity using a Questionnaire

    CERN Document Server

    Sohail, Muhammad

    2009-01-01

    In this paper we present the process of Knowledge Elicitation through a structured questionnaire technique. This is an effort to depict a problem domain as Investigation of factors affecting taskforce productivity. The problem has to be solved using the expert system technology. This problem is the very first step how to acquire knowledge from the domain experts. Knowledge Elicitation is one of the difficult tasks in knowledge base formation which is a key component of expert system. The questionnaire was distributed among 105 different domain experts of Public and Private Organizations (i.e. Education Institutions, Industries and Research etc) in Pakistan. A total 61 responses from these experts were received. All the experts were well qualified, highly experienced and has been remained the members for selection committees a number of times for different posts. Facts acquired were analyzed from which knowledge was extracted and elicited. A standard shape was given to the questionnaire for further research as...

  16. Factors Affecting Adoption of Recommended Cauliflower Production Technology in Nepal

    Directory of Open Access Journals (Sweden)

    Pankaj Raj Dhital

    2016-06-01

    Full Text Available Binary logit regression model of econometrics was used to identify the factors affecting adoption of recommended agricultural technology by the commercial farmers of Nepal. A survey was carried out in 2012 in 120 households from Kavre district, Nepal. The objective was to appraise factors affecting adoption of recommended technology of cauliflower, finding out the level of adoption, identifying the constraints of cauliflower cultivation, assessing the perceived level of satisfaction and studying the relationship of certain selected variables on the adoption. Education, Occupation, contact with Personal Localite sources of information, Group membership and Experience were the most influencing factors for adoption of recommended technology. Though, other factors were not strong enough to contribute significantly but indirectly influence the adoption decision of farmers as combined effects. Occupation was found as negatively associated. Landholding size was strongly and positively influencing among the non significant factors. The level of satisfaction and the constraints of cauliflower production were also identified. Transfer of technology will be effective if and only if the client adopts the technology. For an effective transfer of technology, it is better to go for activities for the welfare of the socioeconomic factors of the farmers.

  17. CRF-like diuretic hormone negatively affects both feeding and reproduction in the desert locust, Schistocerca gregaria.

    Directory of Open Access Journals (Sweden)

    Pieter Van Wielendaele

    Full Text Available Diuretic hormones (DH related to the vertebrate Corticotropin Releasing Factor (CRF have been identified in diverse insect species. In the migratory locust, Locusta migratoria, the CRF-like DH (CRF/DH is localized in the same neurosecretory cells as the Ovary Maturating Parsin (OMP, a neurohormone that stimulates oocyte growth, vitellogenesis and hemolymph ecdysteroid levels in adult female locusts. In this study, we investigated whether CRF-like DH can influence feeding and reproduction in the desert locust, Schistocerca gregaria. We identified two highly similar S. gregaria CRF-like DH precursor cDNAs, each of which also encodes an OMP isoform. Alignment with other insect CRF-like DH precursors shows relatively high conservation of the CRF/DH sequence while the precursor region corresponding to OMP is not well conserved. Quantitative real-time RT-PCR revealed that the precursor transcripts mainly occur in the central nervous system and their highest expression level was observed in the brain. Injection of locust CRF/DH caused a significantly reduced food intake, while RNAi knockdown stimulated food intake. Therefore, our data indicate that CRF-like DH induces satiety. Furthermore, injection of CRF/DH in adult females retarded oocyte growth and caused lower ecdysteroid titers in hemolymph and ovaries, while RNAi knockdown resulted in opposite effects. The observed effects of CRF/DH may be part of a wider repertoire of neurohormonal activities, constituting an integrating control system that affects food intake and excretion, as well as anabolic processes like oocyte growth and ecdysteroidogenesis, following a meal. Our discussion about the functional relationship between CRF/DH and OMP led to the hypothesis that OMP may possibly act as a monitoring peptide that can elicit negative feedback effects.

  18. Factors affecting production rates of cosmogenic nuclides in extraterrestrial matter

    Energy Technology Data Exchange (ETDEWEB)

    Reedy, R.C., E-mail: reedy@psi.edu

    2015-10-15

    Good production rates are needed for cosmic-ray-produced nuclides to interpret their measurements. Rates depend on many factors, especially the pre-atmospheric object’s size, the location of the sample in that object (such as near surface or deep inside), and the object’s bulk composition. The bulk composition affects rates, especially in objects with very low and very high iron contents. Extraterrestrial materials with high iron contents usually have higher rates for making nuclides made by reactions with energetic particles and lower rates for the capture of thermal neutrons. In small objects and near the surface of objects, the cascade of secondary neutrons is being developed as primary particles are being removed. Deep in large objects, that secondary cascade is fully developed and the fluxes of primary particles are low. Recent work shows that even the shape of an object in space has a small but measureable effect. Work has been done and continues to be done on better understanding those and other factors. More good sets of measurements in meteorites with known exposure geometries in space are needed. With the use of modern Monte Carlo codes for the production and transport of particles, the nature of these effects have been and is being studied. Work needs to be done to improve the results of these calculations, especially the cross sections for making spallogenic nuclides.

  19. Initial pH of medium affects organic acids production but do not affect phosphate solubilization

    Directory of Open Access Journals (Sweden)

    Leandro M. Marra

    2015-06-01

    Full Text Available The pH of the culture medium directly influences the growth of microorganisms and the chemical processes that they perform. The aim of this study was to assess the influence of the initial pH of the culture medium on the production of 11 low-molecular-weight organic acids and on the solubilization of calcium phosphate by bacteria in growth medium (NBRIP. The following strains isolated from cowpea nodules were studied: UFLA03-08 (Rhizobium tropici, UFLA03-09 (Acinetobacter sp., UFLA03-10 (Paenibacillus kribbensis, UFLA03-106 (Paenibacillus kribbensis and UFLA03-116 (Paenibacillus sp.. The strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 solubilized Ca3(PO42 in liquid medium regardless of the initial pH, although without a significant difference between the treatments. The production of organic acids by these strains was assessed for all of the initial pH values investigated, and differences between the treatments were observed. Strains UFLA03-09 and UFLA03-10 produced the same acids at different initial pH values in the culture medium. There was no correlation between phosphorus solubilized from Ca3(PO42 in NBRIP liquid medium and the concentration of total organic acids at the different initial pH values. Therefore, the initial pH of the culture medium influences the production of organic acids by the strains UFLA03-08, UFLA03-09, UFLA03-10 and UFLA03-106 but it does not affect calcium phosphate solubilization.

  20. Evaluation of a reproductive index for estimating productivity of grassland breeding birds

    Science.gov (United States)

    Morgan, M.R.; Norment, C.; Runge, M.C.

    2010-01-01

    Declining populations of grassland breeding birds have led to increased efforts to assess habitat quality, typically by estimating density or relative abundance. Because some grassland habitats may function as ecological traps, a more appropriate metric for determining quality is breeding success, which is challenging to determine for many cryptic-nesting grassland birds. This difficulty led Vickery et al. (1992) to propose a reproductive index based on behavioral observations rather than nest fate. We rigorously evaluated the index for 2 years using a Savannah Sparrow (Passerculus sandwichensis) population in western New York and found a weak correlation in classification of the breeding stages of monitored territories among multiple observers (r = 0.398). We also discovered a large difference between overall territory and nest success rates independently estimated with the index (9.8% over the entire breeding cycle) and with nest searching and monitoring (41.7% of nests successfully fledged young). Most importantly, we made territory-level comparisons of index estimates with actual nest fate and found that the index correctly predicted fates for only 43% of the monitored nests. A Mayfield logistic regression analysis demonstrated that only index rank 4 (eggs hatched, but young failed to fledge) showed a strong positive correlation with nest success. Although the reproductive index may function as a coarse indicator of habitat suitability (e.g., documenting production in potential ecological traps), in our study the index exhibited neither internal consistency nor the ability to predict nest fate at the plot or territory level and functioned poorly as a substitute for nest searching and monitoring. ?? 2010 The American Ornithologists' Union.

  1. Cumulative toxicity of an environmentally relevant mixture of nine regulated disinfection by-products in a multigenerational rat reproductive bioassay

    Science.gov (United States)

    CUMULATIVE TOXICITY OF AN ENVIRONMENTALLY RELEVANT MIXTURE OF NINE REGULATED DISINFECTION BY-PRODUCTS IN A MULTIGENERATIONAL RAT REPRODUCTIVE BIOASSAY J E Simmons, GR. Klinefelter, JM Goldman, AB DeAngelo, DS Best, A McDonald, LF Strader, AS Murr, JD Suarez, MH George, ES Hunte...

  2. Reproductive toxicity of a mixture of regulated drinking-water disinfection by-products in a multigenerational rat bioassay

    Science.gov (United States)

    BACKGROUND:Trihalomethanes (THMs) and haloaretic acids (HAAs) are regulated disinfection by-products (DBPs); their joint reproductive toxicity in drinking water is unknown.OBJECTIVE: We aimed to evaluate a drinking water mixture of the four regulated THMs and five regulated HAAs ...

  3. Dam and granddam feeding during pregnancy in sheep affects milk supply in offspring and reproductive performance in grand-offspring.

    Science.gov (United States)

    Blair, H T; Jenkinson, C M C; Peterson, S W; Kenyon, P R; van der Linden, D S; Davenport, L C; Mackenzie, D D S; Morris, S T; Firth, E C

    2010-04-01

    that were fed ad libitum during pregnancy reached puberty at approximately 8 mo of age relative to the other granddam size and feeding groups. These results indicate that dam nutrition can affect the yield and composition of milk in their offspring and the BW and reproductive capability of their grand-offspring. Molecular and physiological mechanisms for these changes are being sought.

  4. Association among gestation length and health, production, and reproduction in Holstein cows and implications for their offspring.

    Science.gov (United States)

    Vieira-Neto, A; Galvão, K N; Thatcher, W W; Santos, J E P

    2017-02-01

    multiparous cows was also greater for AGL than LGL. Heifers from dams with GL that deviated from AGL had greater mortality postweaning (AGL = 3.2 vs. SGL = 6.5 vs. LGL = 5.4%). The rate of removal from the herd was greater for SGL (adjusted hazard ratio = 1.78; 95% CI: 1.26 to 2.52) and LGL (adjusted hazard ratio = 2.00; 95% CI: 1.45 to 2.76) than for AGL heifers. Pregnancy at first AI was lowest for LGL and by 500 d of age a larger proportion of AGL heifers were pregnant than LGL (AGL = 82.3 vs. SGL = 79.2 vs. LGL = 74.0%). Cows with GL within 1 SD of the population mean (270 to 282 d) had improved health, production, and reproduction. Heifers from cows with GL within 1 SD of the population mean had improved health and reproduction. Gestation length affects performance of both dams and their offspring.

  5. Production and Evaluation of Virus-Like Particles Displaying Immunogenic Epitopes of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV

    Directory of Open Access Journals (Sweden)

    Ambika Mosale Venkatesh Murthy

    2015-04-01

    Full Text Available Porcine reproductive and respiratory syndrome (PRRS is the most significant infectious disease currently affecting the swine industry worldwide. Several inactivated and modified live vaccines (MLV have been developed to curb PRRSV infections. However, the efficacy and safety of these vaccines are unsatisfactory, and hence, there is a strong demand for the development of new PRRS universal vaccines. Virus-like particle (VLP-based vaccines are gaining increasing acceptance compared to subunit vaccines, as they present the antigens in a more veritable conformation and are readily recognized by the immune system. Hepatitis B virus core antigen (HBcAg has been successfully used as a carrier for more than 100 viral sequences. In this study, hybrid HBcAg VLPs were generated by fusion of the conserved protective epitopes of PRRSV and expressed in E. coli. An optimized purification protocol was developed to obtain hybrid HBcAg VLP protein from the inclusion bodies. This hybrid HBcAg VLP protein self-assembled to 23-nm VLPs that were shown to block virus infection of susceptible cells when tested on MARC 145 cells. Together with the safety of non-infectious and non-replicable VLPs and the low cost of production through E. coli fermentation, this hybrid VLP could be a promising vaccine candidate for PRRS.

  6. THE INFLUENCE OF THE TECHNOLOGICAL COMFORT ON THE PRODUCTIVE AND REPRODUCTIVE INDICES OF THE QUAILS

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    A. SUMANSCHII

    2013-12-01

    Full Text Available The experiments were held at the quail’s farm „Annotation invest - agro” r. Hâncesti. As a studied material there was used the Japanese bread of quails, kept in six leveled cages, each cage is divided horizontal in half, in that way that in each part there are 35 quails. For the aim achieving there were formed four groups: the first is the control group, and the other three are experimental groups. There was fixed one psychrometer for each group to determine the temperature and the humidity level. There was established the eggs production every day from each group separately. The eggs weight has been determined once in a month, using the electric scales. The egg's index and the egg's shell thickness have been determined by the sliding. The held experiments showed the real influence of the technological factors of the temperature and the humidity of the air on the productive and reproductive indices of the quails grown in conditions of Republic of Moldova.

  7. Water Quality, Essential Condition Sustaining the Health, Production, Reproduction in Cattle. A Review

    Directory of Open Access Journals (Sweden)

    Cristina Iuliana El Mahdy

    2016-11-01

    Full Text Available The main component of the body: the water, alongside with many function which it has,represents a constituent in the diet of animal. There are many and various factors that influence the daily water requirements of animals: some dependent on animal: and others dependent on the environment. Water quality administered to livestock must meet the requirements for potability prerequisite to maintaining the health, externalization full productive potential and sustaining breeding. Knowing the importance of water quality consists in the negative action which can exert on the body to exceeding certain thresholds translated through: reducing water consumption simultaneously with the decrease milk production, decreased feed conversion rate and average daily gain, degradation of health status by reducing the local resistance, decrease overall body resistance, metabolic, digestive, skeletal disorders and impaired reproduction sphere translated through:decreasing fertility, abortions; elements interfering with the absorption of other essential water body, producing chronic or acute poisoning. The water composition plays essential role depending on which is supplemented or not as the case the quantity of the macro and trace minerals from feedingstuff  according to the synergism or antagonism action between  the minerals present.

  8. Mn(II) oxidation by an ascomycete fungus is linked to superoxide production during asexual reproduction.

    Science.gov (United States)

    Hansel, Colleen M; Zeiner, Carolyn A; Santelli, Cara M; Webb, Samuel M

    2012-07-31

    Manganese (Mn) oxides are among the most reactive minerals within the environment, where they control the bioavailability of carbon, nutrients, and numerous metals. Although the ability of microorganisms to oxidize Mn(II) to Mn(III/IV) oxides is scattered throughout the bacterial and fungal domains of life, the mechanism and physiological basis for Mn(II) oxidation remains an enigma. Here, we use a combination of compound-specific chemical assays, microspectroscopy, and electron microscopy to show that a common Ascomycete filamentous fungus, Stilbella aciculosa, oxidizes Mn(II) to Mn oxides by producing extracellular superoxide during cell differentiation. The reactive Mn oxide phase birnessite and the reactive oxygen species superoxide and hydrogen peroxide are colocalized at the base of asexual reproductive structures. Mn oxide formation is not observed in the presence of superoxide scavengers (e.g., Cu) and inhibitors of NADPH oxidases (e.g., diphenylene iodonium chloride), enzymes responsible for superoxide production and cell differentiation in fungi. Considering the recent identification of Mn(II) oxidation by NADH oxidase-based superoxide production by a common marine bacterium (Roseobacter sp.), these results introduce a surprising homology between some prokaryotic and eukaryotic organisms in the mechanisms responsible for Mn(II) oxidation, where oxidation appears to be a side reaction of extracellular superoxide production. Given the versatility of superoxide as a redox reactant and the widespread ability of fungi to produce superoxide, this microbial extracellular superoxide production may play a central role in the cycling and bioavailability of metals (e.g., Hg, Fe, Mn) and carbon in natural systems.

  9. Mn(II) oxidation by an ascomycete fungus is linked to superoxide production during asexual reproduction

    Energy Technology Data Exchange (ETDEWEB)

    Hansel, C. M.; Zeiner, C. A.; Santelli, C. M.; Webb, S. M.

    2012-07-16

    Manganese (Mn) oxides are among the most reactive minerals within the environment, where they control the bioavailability of carbon, nutrients, and numerous metals. Although the ability of microorganisms to oxidize Mn(II) to Mn(III/IV) oxides is scattered throughout the bacterial and fungal domains of life, the mechanism and physiological basis for Mn(II) oxidation remains an enigma. Here, we use a combination of compound-specific chemical assays, microspectroscopy, and electron microscopy to show that a common Ascomycete filamentous fungus, Stilbella aciculosa, oxidizes Mn(II) to Mn oxides by producing extracellular superoxide during cell differentiation. The reactive Mn oxide phase birnessite and the reactive oxygen species superoxide and hydrogen peroxide are colocalized at the base of asexual reproductive structures. Mn oxide formation is not observed in the presence of superoxide scavengers (e.g., Cu) and inhibitors of NADPH oxidases (e.g., diphenylene iodonium chloride), enzymes responsible for superoxide production and cell differentiation in fungi. Considering the recent identification of Mn(II) oxidation by NADH oxidase-based superoxide production by a common marine bacterium (Roseobacter sp.), these results introduce a surprising homology between some prokaryotic and eukaryotic organisms in the mechanisms responsible for Mn(II) oxidation, where oxidation appears to be a side reaction of extracellular superoxide production. Finally, given the versatility of superoxide as a redox reactant and the widespread ability of fungi to produce superoxide, this microbial extracellular superoxide production may play a central role in the cycling and bioavailability of metals (e.g., Hg, Fe, Mn) and carbon in natural systems.

  10. Porcine reproductive and respiratory syndrome virus infection triggers HMGB1 release to promote inflammatory cytokine production

    Energy Technology Data Exchange (ETDEWEB)

    Duan, Erzhen; Wang, Dang; Luo, Rui; Luo, Jingyi; Gao, Li; Chen, Huanchun; Fang, Liurong, E-mail: fanglr@mail.hzau.edu.cn; Xiao, Shaobo, E-mail: vet@mail.hzau.edu.cn

    2014-11-15

    The high mobility group box 1 (HMGB1) protein is an endogenous damage-associated molecular pattern (DAMP) molecule involved in the pathogenesis of various infectious agents. Based on meta-analysis of all publicly available microarray datasets, HMGB1 has recently been proposed as the most significant immune modulator during the porcine response to porcine reproductive and respiratory syndrome virus (PRRSV) infection. However, the function of HMGB1 in PRRSV pathogenesis is unclear. In this study, we found that PRRSV infection triggers the translocation of HMGB1 from the nucleus to the extracellular milieu in MARC-145 cells and porcine alveolar macrophages. Although HMGB1 has no effect on PRRSV replication, HMGB1 promotes PRRSV-induced NF-κB activation and subsequent expression of inflammatory cytokines through receptors RAGE, TLR2 and TLR4. Our findings show that HMGB1 release, triggered by PRRSV infection, enhances the efficiency of virus-induced inflammatory responses, thereby providing new insights into the pathogenesis of PRRSV infection. - Highlights: • PRRSV infection triggers HMGB1 release from MARC-145 cells and PAMs. • HMGB1 does not significantly affect PRRSV proliferation. • HMGB1 is involved in PRRSV-induced NF-κB activation and inflammatory responses. • HMGB1 promotes PRRSV-induced inflammatory responses through TLR2/4 and RAGE.

  11. Improving Productive and Reproductive Performance of Holstein Dairy Cows through Dry Period Management

    Science.gov (United States)

    Safa, S.; Soleimani, A.; Heravi Moussavi, A.

    2013-01-01

    To determine the effects of dry period (DP) length on milk yield, milk composition, some blood metabolites, complete blood count (CBC), body weight and score and follicular status, twenty five primiparous and multiparous Holstein cows were assigned to a completely randomized design with DP-60 (n = 13) and DP-20 (n = 12) dry period lengths. Cows in the DP-60 produced more milk, protein, SNF, serum non-esterified fatty acids (NEFA) and beta hydroxyl butyrate acid (BHBA) compared with cows in DP-20 (p≤0.05). Serum glucose, blood urea nitrogen (BUN), urea, and glutamic oxaloacetic transaminase (GOT) were all similar among the treatments. Body Condition Score (BCS), body weight (BW), complete blood count (CBC) and health problems were similar between the treatments. Diameter of the first dominant follicle and diameter of the dominant follicle on d 14 were different among the treatments. Thus, results of this study showed that reducing the dry period length to DP-20 had a negative effect on milk production, milk composition and reproductive performance in Holstein dairy cows. PMID:25049832

  12. Biotechnology education as social and cultural production/reproduction of the biotechnology community

    Science.gov (United States)

    Andrée, Maria

    2014-03-01

    This paper is a commentary to a paper by Anne Solli, Frank Bach and Björn Åkerman on how students at a technical university learn to argue as biotechnologists. Solli and her colleagues report from an ethnographic study performed during the first semester of a 5-year program in biotechnology at a technical university in Sweden. Their study demonstrates how students begin to acquire `the right way' of approaching the controversial issue of producing and consuming genetically modified organisms. In my response I discuss the ethnographic account of this particular educational practice in terms of social and cultural production/reproduction of a biotechnology community and how the participants (students and teaching professors) deal with the dialectic of individual and collective transformation. In the perspective of the biotechnology community, the work done by the teaching professor becomes a way of ensuring the future of the biotechnology community in terms of what values and objectives are held highly in the community of practice.

  13. Effect of estrogenic activity, and phytoestrogen and organochlorine pesticide contents in an experimental fish diet on reproduction and hepatic vitellogenin production in medaka (Oryzias latipes).

    Science.gov (United States)

    Inudo, Makiko; Ishibashi, Hiroshi; Matsumura, Naomi; Matsuoka, Munekazu; Mori, Taiki; Taniyama, Shigeto; Kadokami, Kiwao; Koga, Minoru; Shinohara, Ryota; Hutchinson, T H; Iguchi, Taisen; Arizono, Koji

    2004-12-01

    Endocrine-disrupting chemicals (EDCs) are giving rise to serious concerns for humans and wildlife. Phytoestrogens, such as daidzein and genistein in plants, and organochlorine pesticides are suspected EDCs, because their chemical structure is similar to that of natural or synthetic estrogens and they have estrogenic activity in vitro and in vivo. We assessed estrogenic activity and dietary phytoestrogen and organochlorine pesticide contents of various fish diets made in the United Kingdom, and compared them with those features of diets made in Japan that were tested in a previous study. Genistein and daidzein were detected in all of the diets. Using an in vitro bioassay, many of these diets had higher activation of estrogen beta-receptors than estrogen alpha-receptors. Organochlorine pesticides such as hexachlorobenzene, beta-benzene hexachloride (BHC), and gamma-BHC were detected in all fish diets. On the basis of these data, we investigated the effect of differing dietary phytoestrogen content in Japanese fish diets on hepatic vitellogenin production and reproduction (fecundity and fertility) in medaka (Oryzias latipes). Assessment of the effects of a 28-day feeding period on reproduction of paired medaka did not indicate significant differences in the number of eggs produced and fertility among all feeding groups. However, hepatic vitellogenin values were significantly higher for male medaka fed diet C (genistein, 58.5 +/- 0.6 microg/g; daidzein, 37.3 +/- 0.2 microg/g) for 28 days compared with those fed diet A (genistein, phytoestrogens, such as diet C, have the potential to induce hepatic vitellogenin production in male medaka, even if reproductive parameters are unaffected. Therefore, some diets, by affecting vitellogenin production in males, may alter estrogenic activity of in vivo tests designed to determine activity of test compounds added to the diet.

  14. Knowledge of Repetitions Range Affects Force Production in Trained Females

    Directory of Open Access Journals (Sweden)

    Israel Halperin, Saied J. Aboodarda, Fabien A. Basset, David G. Behm

    2014-12-01

    Full Text Available Most studies have examined pacing strategies with cyclical activities (running and cycling. It has been demonstrated that males employ different pacing strategies during repeated maximal voluntary contractions (MVCs dependent upon a known endpoint. Since different fatiguing mechanisms have been identified between the genders, it is not known if females use comparable pacing strategies. The purpose of this study was to examine if informing female subjects regarding the number of MVCs to perform would affect force and electromyography (EMG. Twenty well-trained females completed 3 fatiguing protocols in a randomized order. In the control condition participants were informed they would perform twelve MVCs and then actually completed twelve. In the unknown condition they were not told how many MVCs to perform but were stopped after twelve. In the deception condition they were initially informed to perform 6 MVCs, but after the 6th MVC they were asked to perform a few more MVCs and were stopped after twelve. During the first 6 MVCs, forces in the deception condition were greater compared to the unknown (p = 0.021, ES = 0.65, 5% and control (p = 0.022, ES = 0.42, 3% conditions. No differences were found between conditions in the last 6 MVCs. A main effect for repetitions showed force deficits during the first 6 MVCs (p = 0.000, ES = 1.81, 13% and last 6 MVCs (p = 0.05, ES = 0.34, 3%. No differences were found between conditions in biceps and triceps EMG. However, EMG decreased during the first 6 MVCs for biceps (p = 0.001, ES = 1.0, 14% and triceps (p = 0.001, ES = 0.76, 14% across conditions. No differences were found in the last 6 MVCs. The anticipation of performing fewer MVCs led to increased force, whereas no endpoint led to decreased force production.

  15. Sow body condition at weaning and reproduction performance in organic piglet production

    DEFF Research Database (Denmark)

    Kongsted, Anne Grete; Hermansen, John Erik

    2009-01-01

    The objective was to investigate the variation in backfat at weaning and its relations to reproduction results in organic sow herds in Denmark. The study included eight herds and 573 sows. The average backfat at weaning (mean = 13 mm; SD = 4.2 mm) ranging from 10.5 to 17.3 mm among herds shows...... that it is possible to avoid poor body condition at weaning even with a lactation length of seven weeks or more. No main effect of backfat at weaning on reproduction performance was found, but the probability of a successful reproduction after weaning tended to decrease with decreasing backfat for first parity sows...

  16. Emotions, affects and the production of social life.

    Science.gov (United States)

    Fox, Nick J

    2015-06-01

    While many aspects of social life possess an emotional component, sociology needs to explore explicitly the part emotions play in producing the social world and human history. This paper turns away from individualistic and anthropocentric emphases upon the experience of feelings and emotions, attending instead to an exploration of flows of 'affect' (meaning simply a capacity to affect or be affected) between bodies, things, social institutions and abstractions. It establishes a materialist sociology of affects that acknowledges emotions as a part, but only a part, of a more generalized affective flow that produces bodies and the social world. From this perspective, emotions are not a peculiarly remarkable outcome of the confluence of biology and culture, but part of a continuum of affectivity that links human bodies to their physical and social environment. This enhances sociological understanding of the part emotions play in shaping actions and capacities in many settings of sociological concern.

  17. 1962 reproductive success in North American geese: Winter appraisals of productivity

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — An occasional year of poor reproductive success is no novelty among Arctic-nesting birds. Conservationists have become reconciled to this fact, although they still...

  18. Reproductive and productive performances of Santa Inês ewes submitted to breeding in different periods of the Amazonian humid tropical climate.

    Science.gov (United States)

    Soares, Felipe Nogueira; Oliveira, Maria Emilia Franco; Padilha-Nakaghi, Luciana Cristina; de Oliveira, Luís Guilherme; Feliciano, Marcus Antônio Rossi; de Oliveira, Felipe Brener Bezerra; Teixeira, Pedro Paulo Maia; Vicente, Wilter Ricardo Russiano; Faturi, Cristian; Rodrigues, Luiz Fernando de Souza

    2015-12-01

    The objective of this study was to evaluate the reproductive and productive performance of Santa Inês ewes bred at different times of the year in humid tropical climate. One hundred and forty-eight Santa Inês ewes were grouped according to the time of the year of their breeding season (i.e., mating period) (dry/wet, wet, wet/dry, and dry season). The service type was natural mating and the ewes and rams were kept together every night for 45 days. Reproductive efficiency was assessed by service, pregnancy, lambing, prolificacy, twinning, pregnancy loss, weaning, and lamb mortality rates. Ewes were weighed at the beginning and at the end of the breeding season and before and after parturition, and sequential weighing of the lambs was performed (at birth, 15, 30, 60, and 90 days). Reproductive efficiency index (number of lambs weaned/total of served ewes) and productive efficiency (kg of weaned lamb/kg of served or lambed ewes) were calculated. All ewes expressed estrus early in the breeding season; however, a higher percentage (53.5 and 7.1 % at 30 and 45 days, respectively) of ewes returned to estrus during the wet/dry period. The lower rates (13.9 %) of return to estrus at 30 days were during the wet season (P  0.05) effects of breeding seasons on the remaining reproductive rates. Ewes that lambed during the wet/dry transition period weighted less, before (40.5 ± 2.5 kg) and after (38.6 ± 1.6 kg) parturition, than those of other groups (P breeding season took place during the dry/wet period (P breeding seasons took place, allowing for four breeding seasons a year in the Amazon region. Variations between periods in return to estrus rates, weight of ewes close to parturition and lamb weight at weaning indicate that climate changes can also affect reproductive rates.

  19. EFFECTS OF HARVESTING ON LEAF PRODUCTION AND REPRODUCTIVE PERFORMANCE OF Copernicia prunifera (Mill. H.E. Moore1

    Directory of Open Access Journals (Sweden)

    Irlaine Rodrigues Vieira

    2016-02-01

    Full Text Available ABSTRACT This work aimed to evaluate the consequences of the monthly extraction of immature leaves in survival, leaf production and reproductive performance ofCopernicia prunifera H. E. Moore palm, popularly known as carnaúba. One hundred sixty reproductive adult palms were monitored for 17 months in four extractive communities located at the coast of the state of Piauí. As a result, it was observed that leaves, flowers, fruits and seeds production were reduced in the palm submitted to 50% or 75% monthly extraction. Higher levels of extraction were followed by smaller levels in seed germination. No deaths were observed even in the group subjected to 75% monthly leaves exploration. In order not to produce damage to palm trees development it is suggested that leaf extraction rate should not exceed 25% monthly as well as pausing of extractive activity preferentially during fruit maturation.

  20. Prediction of the lifetime productive and reproductive performance of Holstein cows managed for different lactation durations, using a model of lifetime nutrient partitioning

    DEFF Research Database (Denmark)

    Gaillard, Charlotte; Martin, O; Blavy, P

    2016-01-01

    The GARUNS model is a lifetime performance model taking into account the changing physiological priorities of an animal during its life and through repeated reproduction cycles. This dynamic and stochastic model has been previously used to predict the productive and reproductive performance of va...

  1. High levels of maternally transferred mercury do not affect reproductive output or embryonic survival of northern watersnakes (Nerodia sipedon).

    Science.gov (United States)

    Chin, Stephanie Y; Willson, John D; Cristol, Daniel A; Drewett, David V V; Hopkins, William A

    2013-03-01

    Maternal transfer is an important exposure pathway for contaminants because it can directly influence offspring development. Few studies have examined maternal transfer of contaminants, such as mercury (Hg), in snakes, despite their abundance and high trophic position in many ecosystems where Hg is prevalent. The objectives of the present study were to determine if Hg is maternally transferred in northern watersnakes (Nerodia sipedon) and to evaluate the effects of maternal Hg on reproduction. The authors captured gravid female watersnakes (n = 31) along the South River in Waynesboro, Virginia, USA, where an extensive Hg-contamination gradient exists. The authors measured maternal tissue and litter Hg concentrations and, following birth, assessed (1) reproductive parameters (i.e., litter size and mass, neonate mass); (2) rates of infertility, death during development, stillbirths, malformations, and runts; and (3) the overall viability of offspring. Mercury concentrations in females were strongly and positively correlated with concentrations in litters, suggesting that N. sipedon maternally transfer Hg in proportion to their tissue residues. Maternal transfer resulted in high concentrations (up to 10.10 mg/kg dry wt total Hg) of Hg in offspring. The authors found little evidence of adverse effects of Hg on these measures of reproductive output and embryonic survival, suggesting that N. sipedon may be more tolerant of Hg than other vertebrate species. Given that this is the first study to examine the effects of maternally transferred contaminants in snakes and that the authors did not measure all reproductive endpoints, further research is needed to better understand factors that influence maternal transfer and associated sublethal effects on offspring.

  2. Detection of genetic variants affecting cattle behaviour and their impact on milk production: a genome-wide association study.

    Science.gov (United States)

    Friedrich, Juliane; Brand, Bodo; Ponsuksili, Siriluck; Graunke, Katharina L; Langbein, Jan; Knaust, Jacqueline; Kühn, Christa; Schwerin, Manfred

    2016-02-01

    Behaviour traits of cattle have been reported to affect important production traits, such as meat quality and milk performance as well as reproduction and health. Genetic predisposition is, together with environmental stimuli, undoubtedly involved in the development of behaviour phenotypes. Underlying molecular mechanisms affecting behaviour in general and behaviour and productions traits in particular still have to be studied in detail. Therefore, we performed a genome-wide association study in an F2 Charolais × German Holstein cross-breed population to identify genetic variants that affect behaviour-related traits assessed in an open-field and novel-object test and analysed their putative impact on milk performance. Of 37,201 tested single nucleotide polymorphism (SNPs), four showed a genome-wide and 37 a chromosome-wide significant association with behaviour traits assessed in both tests. Nine of the SNPs that were associated with behaviour traits likewise showed a nominal significant association with milk performance traits. On chromosomes 14 and 29, six SNPs were identified to be associated with exploratory behaviour and inactivity during the novel-object test as well as with milk yield traits. Least squares means for behaviour and milk performance traits for these SNPs revealed that genotypes associated with higher inactivity and less exploratory behaviour promote higher milk yields. Whether these results are due to molecular mechanisms simultaneously affecting behaviour and milk performance or due to a behaviour predisposition, which causes indirect effects on milk performance by influencing individual reactivity, needs further investigation.

  3. The effect of continuous feeding of gliricidia on reproduction and production performances of Javanese Fat-Tailed sheep: Impact on the second breeding

    Directory of Open Access Journals (Sweden)

    Supriyati

    1999-10-01

    Full Text Available The effect of continuous feeding of gliricidia on reproduction and production performances in second breeding of Javanese Fat-Tailed (JFT sheep through the second breeding period was studied. Twenty six ewes post lactation at first lambing (body weight 22.5-26.5 kg were divided into four treatment groups. Each group consisted of 6 animals except Group D had 8 animals. They were given King grass (Pennisetum purpureophoides and gliricidia (Gliricidia sepium Jacq. leaf with ratio of 100:0 (Group A = control, 75:25 (Group B, 50:50 (Group C and 0:100 (Group D. Forages were given 2.5-3% (dry matter of liveweight. All groups were supplemented with 100g/head/day concentrate (crude protein = 16%, but during late pregnancy and lactation they were supplemented at 200 g/head/day. Results showed that feeding gliricidia 25-100% of total forages improved body weight due to the increasing protein intake. The maximum concentration of progesteron increased from 0.81 to 2.78 ng/ml. The ovulation rate and prolification also increased significantly (P<0.05 from 1.6 to 3.0 and 1.33 to 2.38, respectively. Individual birth and weaning weights of the lambs were not affected. It is concluded that feeding gliricidia continuously up to 100% as forages and supplemented with concentrate gives positive effect on bodyweight gain, reproduction and production performances in second breeding of JFT sheep.

  4. Paternal obesity negatively affects male fertility and assisted reproduction outcomes: a systematic review and meta-analysis.

    Science.gov (United States)

    Campbell, Jared M; Lane, Michelle; Owens, Julie A; Bakos, Hassan W

    2015-11-01

    This systematic review investigated the effect of paternal obesity on reproductive potential. Databases searched were Pubmed, Ovid, Web of Science, Scopus, Cinahl and Embase. Papers were critically appraised by two reviewers, and data were extracted using a standardized tool. Outcomes were: likelihood of infertility, embryo development, clinical pregnancy, live birth, pregnancy viability, infant development, sperm; concentration, morphology, motility, volume, DNA fragmentation, chromatin condensation, mitochondrial membrane potential (MMP), and seminal plasma factors. Thirty papers were included, with a total participant number of 115,158. Obese men were more likely to experience infertility (OR = 1.66, 95% CI 1.53-1.79), their rate of live birth per cycle of assisted reproduction technology (ART) was reduced (OR = 0.65, 95% CI 0.44-0.97) and they had a 10% absolute risk increase of pregnancy non-viability. Additionally, obese men had an increased percentage of sperm with low MMP, DNA fragmentation, and abnormal morphology. Clinically significant differences were not found for conventional semen parameters. From these findings it can be concluded that male obesity is associated with reduced reproductive potential. Furthermore, it may be informative to incorporate DNA fragmentation analysis and MMP assessment into semen testing, especially for obese men whose results suggest they should have normal fertility.

  5. [The students' page. Notes about patient records and the production and reproduction of knowledge. Written and oral presentations].

    Science.gov (United States)

    de Almeida, L B; dos Santos, E S; Alves, D de B

    1995-01-01

    This paper relates a description about nursing notes in pactients promptuaries in relation to the distribution/reproduction/production process of knowledge in nursing. It was developed on an Intensive Care Unity from a Hospital in the teaching/assistance Sanitary District Barra/Rio Vermelho (Salvador-Bahia). The basic premise is that the nursing personal work superficial and sporadic notes just related to patients. Distribution/ reproduction/production process, that happen in the nursing work process isn't considered by it devaluation or just because, for nursing agents, only information about technical procedures related to patient care, expresses the nursing knowledge and so, must be registered. In order to reach the objective, 30% of the promptuaries of patients discharge from the ICU were studied and the nursing team was interviewed, during the months-november, december 1994. The analysis indicates that the way how the nursing notes are been made, results in hard communication among agents of health area and contributes to disqualify the nursing assistance that is given to patients, besides to limit the advance of the distribution/reproduction/production of knowledge in nursing.

  6. Discourse Goals Affect the Process and Product of Nominal Metaphor Production.

    Science.gov (United States)

    Utsumi, Akira; Sakamoto, Maki

    2015-10-01

    Although a large number of studies have addressed metaphor comprehension, only a few attempts have so far been made at exploring the process of metaphor production. Therefore, in this paper, we address the problem of how people generate nominal metaphors or identify an apt vehicle for a given topic of nominal metaphors. Specifically, we examine how the process and product of metaphor production differ between two discourse goals of metaphor, namely an explanatory purpose (e.g., to clarify) and a literary purpose (e.g., to aesthetically pleasing). Experiment 1 analyzed the metaphors (or vehicles) generated in the metaphor production task, and demonstrated that people identified more prototypical exemplars of the property to be attributed to the topic as a vehicle for explanatory metaphors than for literary metaphors. In addition, it was found that metaphors generated for the explanatory purpose were more apt and conventional, and had high topic-vehicle similarity than those generated for the literary purpose, while metaphors generated for the literary purpose were more familiar and imageable than those for the explanatory purpose. Experiment 2 used a priming paradigm to assess the online availability of prototypical and less prototypical members of the topic property during metaphor production. The result was that both prototypical and less prototypical members were activated in producing literary metaphors, while neither members were activated in the production of explanatory metaphors. These findings indicate that the process of metaphor production is affected by discourse goals of metaphor; less prototypical members of the category are searched for a vehicle during the production of literary metaphors, and thus literary metaphors are generated with less prototypical vehicles than explanatory metaphors.

  7. REPEATABILITY ESTIMATES OF SOME PRODUCTIVE AND REPRODUCTIVE TRAITS IN RED SINDHI CATTLE

    Directory of Open Access Journals (Sweden)

    Muhammad Iqbal Mustafa, Muhammad Khalid Bashir1, Arfan Yousaf2 and Bashir Ahmad

    2002-03-01

    Full Text Available The breeding records of 179 Red Sindhi cattle maintained at the Livestock Experiment Station Hab Choki, Balochistan, during the years 1982-97 were used in the present study. An effort was made to estimate the repeatability for some productive and reproductive traits by using the Mixed model least squares and maximum likelihood procedures. The repeatability estimates for milk yield, lactation length, dry period, service period, gestation period and calving interval were 0.361 ± 0.05, 0.03 ± 0.05, 0.029 ± 0.06, 0.17± 0.06, 0.94 ± 0.55 and 0.167 ± 0.06, respectively in model 1, whereas in model 2 estimates for milk yield, lactation length, dry period, Service period, gestation period and calving interval were 0.354 ± 0.05. 0.041 ± 0.05, 0.040 ± 0.06, 0.173 ± 0.06, 0.98 ± 0.056 and 0.165 ± 0.06, respectively. The repeatability estimates are used in determining the amount of culling that can be safely done on the basis of records. A high estimate of repeatability provides enough evidence for selection or rejection of the individual based on single record. Whereas a low estimate of repeatability justifies accumulation of further records. A moderate estimate of repeatability (0.361 for lactation milk yield obtained in the present study indicates that the cows can be selected for milk yield on the basis of relatively lesser records in the present herd.

  8. Is the reproduction of Donax trunculus affected by their sites of origin contrasted by their level of contamination?

    Science.gov (United States)

    Tlili, Sofiène; Métais, Isabelle; Ayache, Nadia; Boussetta, Hamadi; Mouneyrac, Catherine

    2011-09-01

    The reproductive cycle of bivalves is regulated by several natural environmental factors but exposure to chemical pollutants can also interfere and may result in advanced or delayed spawning season. To our knowledge, the gametogenic cycle of the suspension-feeder bivalve Donax trunculus has not yet been used as biomonitoring tool in ecotoxicological surveys. The aim of this study was to examine over a year physiological reproductive endpoints (sex-ratio, gametogenic and energy reserve cycles) and biological indices (condition index, allometry) in D. trunculus originating from two sites differing by their level of contamination. Specimens were collected bimonthly from November 2008 to October 2009 from a polluted site (Radès Méliane) and a comparatively reference site (Sidi Jehmi) in the Gulf of Tunis (Tunisia). Five stages were depicted by histological examination of gonads: undifferentiated, developing, mature, spawn and spent. Differences in the gametogenic cycle according to the site of origin of bivalves were observed. The spawning period began in March and was maximum in May in bivalves from both sites, but the percentage of spawning animals was higher in the polluted site vs the reference site. The spawning period was shorter in animals from the polluted site comparatively to the reference site. Energy reserves (glycogen, lipids) were higher in March and May comparatively to the other studied periods in bivalves from both sites. Lower energy reserves levels were usually observed in animals from the polluted site compared to the reference site. Seasonal variations of the condition index were associated to the reproductive and nutritive status of bivalves. Differences in allometry were depicted between bivalves from both studied sites. If we try to link allometry, energy reserves and reproduction, it can be hypothesized that for bivalves from the reference site, energy reserves are allocated to gametogenesis and length growth. For bivalves from the polluted

  9. Reassessing culture media and critical metabolites that affect adenovirus production.

    Science.gov (United States)

    Shen, Chun Fang; Voyer, Robert; Tom, Roseanne; Kamen, Amine

    2010-01-01

    Adenovirus production is currently operated at low cell density because infection at high cell densities still results in reduced cell-specific productivity. To better understand nutrient limitation and inhibitory metabolites causing the reduction of specific yields at high cell densities, adenovirus production in HEK 293 cultures using NSFM 13 and CD 293 media were evaluated. For cultures using NSFM 13 medium, the cell-specific productivity decreased from 3,400 to 150 vp/cell (or 96% reduction) when the cell density at infection was increased from 1 to 3 x 10(6) cells/mL. In comparison, only 50% of reduction in the cell-specific productivity was observed under the same conditions for cultures using CD 293 medium. The effect of medium osmolality was found critical on viral production. Media were adjusted to an optimal osmolality of 290 mOsm/kg to facilitate comparison. Amino acids were not critical limiting factors. Potential limiting nutrients including vitamins, energy metabolites, bases and nucleotides, or inhibitory metabolites (lactate and ammonia) were supplemented to infected cultures to further investigate their effect on the adenovirus production. Accumulation of lactate and ammonia in a culture infected at 3 x 10(6) cells/mL contributed to about 20% reduction of the adenovirus production yield, whereas nutrient limitation appeared primarily responsible for the decline in the viral production when NSFM 13 medium was used. Overall, the results indicate that multiple factors contribute to limiting the specific production yield at cell densities beyond 1 x 10(6) cells/mL and underline the need to further investigate and develop media for better adenoviral vector productions.

  10. Effect of broiler breeders fed with corn or sorghum diet and canthaxanthin supplementation on production and reproductive performance.

    Science.gov (United States)

    Bonilla, C E V; Rosa, A P; Londero, A; Giacomini, C B S; Orso, C; Fernandes, M O; Paixão, S J; Bonamigo, D V

    2017-03-04

    This study was designed to evaluate the effect of corn or sorghum diet and canthaxanthin (CX) supplementation on performance in broiler breeders. A total of 440 females with similar body weight (BW) (3.71 ± 0.14 kg) and 60 roosters were placed in an open-sided house with 20 pens, randomly distributed in a factorial arrangement (2 × 2). There were 4 diets of 2 ingredients; corn (CO) or sorghum (SO) and 2 levels of CX; 6 mg/kg (CX) and 0 mg/kg (NCX) totaling 5 replicate pens of 22 females and 3 males each, from 42 to 65 wk, divided in 2 periods (from wk 42 to 53 and wk 54 to 65). Birds' BW was measured every 28 d and mortality rate was calculated at the end of trial. Egg production (%), egg specific gravity (g/cm3), egg weight (g), yolk weight (%), albumen weight (%), eggshell weight (%) and yolk colorimetric score were measured weekly. Incubation parameters were recorded in 12 incubations to evaluate hatching eggs, hatching (%), hatchability (%), fertility (%), weight of the chicks born and their quality. The BW, mortality, percentage of yolk and albumen weight, fertility and some incubation parameters were not affected (P > 0.05) by diets used. An increase in the egg production, hatching eggs, chicks born and first quality chick by hen at the second period were observed in CX breeder's diets (P = 0.0066; P = 0.0060; P = 0.0368; P = 0.0326). Egg specific gravity and eggshell weight were improved at the first period by SO+CX diet (P = 0.0138; P = 0.0209) and the same effect to egg weight, but at the second period (P = 0.0251). The CX was well absorbed from the diet and effectively transferred to the egg yolk, thereby increasing egg yolk pigmentation in the both periods (P < 0.0001). The CX supplementation in broiler breeder diets improved the productive and reproductive performance (laying% and hatchable eggs) at the second period, also to the both periods improved the egg yolk pigmentation.

  11. The African cichlid fish Astatotilapia burtoni uses acoustic communication for reproduction: sound production, hearing, and behavioral significance.

    Science.gov (United States)

    Maruska, Karen P; Ung, Uyhun S; Fernald, Russell D

    2012-01-01

    Sexual reproduction in all animals depends on effective communication between signalers and receivers. Many fish species, especially the African cichlids, are well known for their bright coloration and the importance of visual signaling during courtship and mate choice, but little is known about what role acoustic communication plays during mating and how it contributes to sexual selection in this phenotypically diverse group of vertebrates. Here we examined acoustic communication during reproduction in the social cichlid fish, Astatotilapia burtoni. We characterized the sounds and associated behaviors produced by dominant males during courtship, tested for differences in hearing ability associated with female reproductive state and male social status, and then tested the hypothesis that female mate preference is influenced by male sound production. We show that dominant males produce intentional courtship sounds in close proximity to females, and that sounds are spectrally similar to their hearing abilities. Females were 2-5-fold more sensitive to low frequency sounds in the spectral range of male courtship sounds when they were sexually-receptive compared to during the mouthbrooding parental phase. Hearing thresholds were also negatively correlated with circulating sex-steroid levels in females but positively correlated in males, suggesting a potential role for steroids in reproductive-state auditory plasticity. Behavioral experiments showed that receptive females preferred to affiliate with males that were associated with playback of courtship sounds compared to noise controls, indicating that acoustic information is likely important for female mate choice. These data show for the first time in a Tanganyikan cichlid that acoustic communication is important during reproduction as part of a multimodal signaling repertoire, and that perception of auditory information changes depending on the animal's internal physiological state. Our results highlight the

  12. Early social instability affects plasma testosterone during adolescence but does not alter reproductive capacity or measures of stress later in life.

    Science.gov (United States)

    Siegeler, Katja; Wistuba, Joachim; Damm, Oliver S; von Engelhardt, Nikolaus; Sachser, Norbert; Kaiser, Sylvia

    2013-08-15

    The social environment plays an important role in modulating processes of the hormonal and behavioural profile of an animal in a variety of group-living species. In wild cavies for instance, unstable social environmental conditions during pregnancy and lactation lead to an infantilised biobehavioural profile of the male offspring. In the present study, the influence of the social environment during pregnancy and lactation on the male wild cavy offsprings' plasma testosterone development, reproductive capacity and stress system activity was investigated. To this purpose, 12 sons whose mothers had lived in an unstable social environment during pregnancy and lactation were compared with 12 sons whose mothers had lived in a stable social environment during the same time. Plasma testosterone (T) and plasma cortisol (C) concentrations were determined from days 20 to 107 of age. Adrenal tyrosine hydroxylase (TH) activity and different parameters of reproductive capacity (weights of testes, epididymides and accessory sex glands, cellular composition of the testes, DNA fragmentation indices and sperm motility parameters) were analysed at day 107 of age. TH activity and plasma C were unaffected by different social environmental conditions early in life. The developmental time course of T concentrations, however, was significantly different: Sons whose mothers had lived in an unstable social environment during pregnancy and lactation showed a delayed increase in T concentrations around adolescence compared to controls. In contrast, no reproduction-related parameters measured within this study differed significantly between the two groups. Thus, early social instability affects plasma testosterone development during adolescence in a significant way but does not alter reproductive capacity or measures of stress later in life.

  13. Statistical frequency in perception affects children's lexical production.

    Science.gov (United States)

    Richtsmeier, Peter T; Gerken, LouAnn; Goffman, Lisa; Hogan, Tiffany

    2009-06-01

    Children's early word production is influenced by the statistical frequency of speech sounds and combinations. Three experiments asked whether this production effect can be explained by a perceptual learning mechanism that is sensitive to word-token frequency and/or variability. Four-year-olds were exposed to nonwords that were either frequent (presented 10 times) or infrequent (presented once). When the frequent nonwords were spoken by the same talker, children showed no significant effect of perceptual frequency on production. When the frequent nonwords were spoken by different talkers, children produced them with fewer errors and shorter latencies. The results implicate token variability in perceptual learning.

  14. Reproductive conflict in social insects: Male production by workers in a slave-making ant

    DEFF Research Database (Denmark)

    Brunner, Elizabeth; Trindl, Andreas; Falk, Karl H.

    2005-01-01

    AbstractIn insect societies, workers cooperate but may also pursue their individual interests, such as laying viable male eggs. The case of obligatory slave-making ants is of particular interest because workers do not engage in maintenance activities and foraging. Therefore, worker egg laying...... by producing their own sons than workers in nonparasitic species. In this study we investigated worker reproduction in four natural colonies of the slave-making ant Polyergus rufescens, using highly variable microsatellite markers. Our results show that workers produce up to 100% of the males. This study thus...... presents the first direct evidence of an almost complete takeover of male reproduction by workers in ants....

  15. Are introspective reaction times affected by the method of time estimation? A comparison of visual analogue scales and reproduction.

    Science.gov (United States)

    Bryce, Donna; Bratzke, Daniel

    2015-04-01

    In this study, we investigated whether the method of time estimation plays a role in the apparent limits of introspection in dual-task processing. Previous studies showed that when participants reported introspective reaction times after each trial of a dual task by clicking on a visual analogue scale, they appeared to be unaware of the dual-task costs in their performance. However, visual analogue scales have seldom been used in interval estimation, and they may be inappropriate. In the present study, after each dual-task trial, participants reported their introspective reaction times either via a visual analogue scale or via the method of reproduction. The results replicated the previous findings, irrespective of method. That is, even though responses to the second task slowed down with increasing task overlap, this slowing was only very weakly reflected in the introspective reaction times. Thus, the participants' failure to report the objective dual-task costs in their reaction times is a rather robust finding that cannot be attributed to the method employed. However, introspective reaction times reported via visual analogue scales were more closely related to the objective reaction times, suggesting that visual analogue scales are preferable to reproduction. We conclude that introspective reaction times represent the same information regardless of method, but whether that information is temporal in nature is as yet unsettled.

  16. Nest-site selection and reproductive success of greater sage-grouse in a fire-affected habitat of northwestern Nevada

    Science.gov (United States)

    Lockyer, Zachary B.; Coates, Peter S.; Casazza, Michael L.; Espinosa, Shawn; Delehanty, David J.

    2015-01-01

    Identifying links between micro-habitat selection and wildlife reproduction is imperative to population persistence and recovery. This information is particularly important for landscape species such as greater sage-grouse (Centrocercus urophasianus; sage-grouse). Although this species has been widely studied, because environmental factors can affect sage-grouse populations, local and regional studies are crucial for developing viable conservation strategies. We studied the habitat-use patterns of 71 radio-marked sage-grouse inhabiting an area affected by wildfire in the Virginia Mountains of northwestern Nevada during 2009–2011 to determine the effect of micro-habitat attributes on reproductive success. We measured standard vegetation parameters at nest and random sites using a multi-scale approach (range = 0.01–15,527 ha). We used an information-theoretic modeling approach to identify environmental factors influencing nest-site selection and survival, and determine whether nest survival was a function of resource selection. Sage-grouse selected micro-sites with greater shrub canopy cover and less cheatgrass (Bromus tectorum) cover than random sites. Total shrub canopy, including sagebrush (Artemisia spp.) and other shrub species, at small spatial scales (0.8 ha and 3.1 ha) was the single contributing selection factor to higher nest survival. These results indicate that reducing the risk of wildfire to maintain important sagebrush habitats could be emphasized in sage-grouse conservation strategies in Nevada. Managers may seek to mitigate the influx of annual grass invasion by preserving large intact sagebrush-dominated stands with a mixture of other shrub species. For this area of Nevada, the results suggest that ≥40% total shrub canopy cover in sage-grouse nesting areas could yield improved reproductive success. 

  17. Analysis of Management Behavior Assessments and Affect on Productivity

    Science.gov (United States)

    2005-06-10

    associated with the affects of management behavior on employee beliefs and attitudes; include Herzberg’s Two Factor Theory , Vroom’s Expectancy and Equity...achieved, when recognized, when given additional responsibility, and when advanced in job position. Vroom’s Expectancy and Equity Theories of Motivation...degree or higher. The results of the study proved the validity of the Vroom and Yetton theory , but the employee assessments of management decision-making

  18. Temperature can interact with landscape factors to affect songbird productivity.

    Science.gov (United States)

    Cox, W Andrew; Thompson, Frank R; Reidy, Jennifer L; Faaborg, John

    2013-04-01

    Increased temperatures and more extreme weather patterns associated with global climate change can interact with other factors that regulate animal populations, but many climate change studies do not incorporate other threats to wildlife in their analyses. We used 20 years of nest-monitoring data from study sites across a gradient of habitat fragmentation in Missouri, USA, to investigate the relative influence of weather variables (temperature and precipitation) and landscape factors (forest cover and edge density) on the number of young produced per nest attempt (i.e., productivity) for three species of songbirds. We detected a strong forest cover × temperature interaction for the Acadian Flycatcher (Empidonax virescens) on productivity. Greater forest cover resulted in greater productivity because of reduced brood parasitism and increased nest survival, whereas greater temperatures reduced productivity in highly forested landscapes because of increased nest predation but had no effect in less forested landscapes. The Indigo Bunting (Passerina cyanea) exhibited a similar pattern, albeit with a marginal forest cover × temperature interaction. By contrast, productivity of the Northern Cardinal (Cardinalis cardinalis) was not influenced by landscape effects or temperature. Our results highlight a potential difficulty of managing wildlife in response to global change such as habitat fragmentation and climate warming, as the habitat associated with the greatest productivity for flycatchers was also that most negatively influenced by high temperatures. The influence of high temperatures on nest predation (and therefore, nest predators) underscores the need to acknowledge the potential complexity of species' responses to climate change by incorporating a more thorough consideration of community ecology in the development of models of climate impacts on wildlife.

  19. Productive Discomfort: Dialogue, Reproductive Choice and Social Justice Education at the Matilda Joslyn Gage Center

    Science.gov (United States)

    Roesch Wagner, Sally; Eckler, Tori; Leighton, Maxinne Rhea

    2013-01-01

    Museums in the past have been static institutions, exhibiting their collections as public displays. Today, the public has come to expect more from these institutions, seeing them as safe havens where conversations can begin. As reproductive rights have moved to the forefront of political and social debate, dialogue seems to be a step in the right…

  20. Potato (Solanum tuberosum) greenhouse tuber production as an assay for asexual reproduction effects from herbicides

    Science.gov (United States)

    The present study determined whether young potato plants can be used as an assay to indicate potential effects of pesticides on asexual reproduction. Solanum tuberosum (Russet Burbank) plants were grown from seed pieces in a mineral soil in pots under greenhouse conditions. Plant...

  1. The Natural Reproduction and Economic Reproduction of Agriculture

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    In the perspective of Marx’s materialism,the thesis expounds the natural reproduction and economic reproduction of agriculture,and analyzes the relationship between the two production processes.The natural reproduction of agriculture,the contradicted unification of agricultural organism and natural environment,conforms to natural law and has the specific objective environmental requirements.The economic reproduction of agriculture is the process of people’s production and labor which takes keeping the life vitality of agricultural organism as objective,including the reproduction of agricultural productivity and the reproduction of agricultural production relations.The agricultural productivity comprises social productivity and natural productivity.The process of agricultural economic reproduction must conform to the objective natural law and economic law.The relationship of agricultural natural reproduction and economic reproduction is that the agricultural natural reproduction process is intertwined with economic reproduction process;the variation trend of agricultural natural reproduction ability and economic reproduction ability is identical;the agricultural economic reproduction dominates over the natural reproduction.

  2. Sex of littermate twin affects lifetime ewe productivity

    Science.gov (United States)

    Ewe productivity is synonymous with annual litter-weight weaned (LWW) per ewe exposed to rams for breeding, and LWW is largely a function of number of lambs born (NLB) and weaned (NLW). Selecting for LWW should increase litter size and numbers of ewe-ram co-twins. Thus, we used historical records to...

  3. A Content Analysis of Factors Affecting New Product Development Process

    Directory of Open Access Journals (Sweden)

    Eda Atilgan-Inan

    2010-07-01

    Full Text Available The objective of this study is to review the international marketing literature on new product development process and compare the changes in the important factors in the process with the changes in the management approaches. For this purpose, the articles in three international marketing journals were selected and “new product development” and “new product performance” were searched for in the abstracts. After grouping the variables in the process, they were compared with the perspectives of management in the related periods. The results indicated that organizational factors have always been important for new product development process, which is in line with the nature of the innovation process. But the emphasis on internal factors has increased in the 21st century which is congruent with the change in management perspective foregrounding resource based view. The study differs from the similar literature review studies on the point that it deals with the topic from international marketing perspective. Therefore, R&D and other marketing studies are not included in the review and the study proposes the important factors from international firms’ point of view.

  4. Affected functional networks associated with sentence production in classic galactosemia.

    Science.gov (United States)

    Timmers, Inge; van den Hurk, Job; Hofman, Paul Am; Zimmermann, Luc Ji; Uludağ, Kâmil; Jansma, Bernadette M; Rubio-Gozalbo, M Estela

    2015-08-07

    Patients with the inherited metabolic disorder classic galactosemia have language production impairments in several planning stages. Here, we assessed potential deviations in recruitment and connectivity across brain areas responsible for language production that may explain these deficits. We used functional magnetic resonance imaging (fMRI) to study neural activity and connectivity while participants carried out a language production task. This study included 13 adolescent patients and 13 age- and gender-matched healthy controls. Participants passively watched or actively described an animated visual scene using two conditions, varying in syntactic complexity (single words versus a sentence). Results showed that patients recruited additional and more extensive brain regions during sentence production. Both groups showed modulations with syntactic complexity in left inferior frontal gyrus (IFG), a region associated with syntactic planning, and in right insula. In addition, patients showed a modulation with syntax in left superior temporal gyrus (STG), whereas the controls did not. Further, patients showed increased activity in right STG and right supplementary motor area (SMA). The functional connectivity data showed similar patterns, with more extensive connectivity with frontal and motor regions, and restricted and weaker connectivity with superior temporal regions. Patients also showed higher baseline cerebral blood flow (CBF) in right IFG and trends towards higher CBF in bilateral STG, SMA and the insula. Taken together, the data demonstrate that language abnormalities in classic galactosemia are associated with specific changes within the language network. These changes point towards impairments related to both syntactic planning and speech motor planning in these patients.

  5. Reproductive and productive efficiencies of Etawah Grade goats under various mating managements

    Directory of Open Access Journals (Sweden)

    Bambang Sunadi

    1998-12-01

    Full Text Available Thirty six Etawah Grade (PE goats were treated with three type of mating managements, i.e. mated at the first oestrous (A, mated at the second oestrous (B, and mated at the third oestrous (C after parturition, respectively . Results showed that average first estrous was 56 days (26-99 d after parturition with estrous cycle of 21 days . Conception rate at the first and second oestrous mating managements (A and B were 50 and 70%, respectively . Variability of birth weight (3,4 - 3,5 kg under three mating managements were not significantly different (P>0 .05, but the weaning weight of kids of B (16 .4 kg was higher (P<0.05 than A (11 .8 kg and C (12.9 kg, respectively. Does productivity (total weaning weight was not significantly affected by mating management, i.e. at fisrt, second or third oestrous after parturition .

  6. Relations among Individual Differences in Reproductive Strategies, Sexual Attractiveness, Affective and Punitive Intentions, and Imagined Sexual or Emotional Infidelity

    Directory of Open Access Journals (Sweden)

    Daniel N. Jones

    2007-04-01

    Full Text Available We examined relations among Mating Effort, Mate Value, Sex and individuals' self-reported responses to imagined sexual or emotional infidelity. We asked participants to describe the (1 upset or bother (2 aversive emotional reactions (3 punitive impulses, and (4 punitive intentions they experienced in response to imagined sexual or emotional infidelity. The results replicated previously documented sex differences in jealousy. In addition, imagined sexual infidelity upset individuals higher in Mating Effort more than those lower in Mating Effort. Higher Mating Effort also predicted greater temptation, intention, and likelihood to engage in punitive behaviors in response to imagined sexual or emotional infidelity. We discuss these data in light of individual differences in relations between reproductive strategy and romantic jealousy. Additionally, we point to the importance of controlling for co-linearity between reactions to sexual and emotional infidelity, and the need for addressing related methodological problems within jealousy research.

  7. The sleeping beauty: how reproductive diapause affects hormone signaling, metabolism, immune response and somatic maintenance in Drosophila melanogaster.

    Science.gov (United States)

    Kubrak, Olga I; Kučerová, Lucie; Theopold, Ulrich; Nässel, Dick R

    2014-01-01

    Some organisms can adapt to seasonal and other environmental challenges by entering a state of dormancy, diapause. Thus, insects exposed to decreased temperature and short photoperiod enter a state of arrested development, lowered metabolism, and increased stress resistance. Drosophila melanogaster females can enter a shallow reproductive diapause in the adult stage, which drastically reduces organismal senescence, but little is known about the physiology and endocrinology associated with this dormancy, and the genes involved in its regulation. We induced diapause in D. melanogaster and monitored effects over 12 weeks on dynamics of ovary development, carbohydrate and lipid metabolism, as well as expression of genes involved in endocrine signaling, metabolism and innate immunity. During diapause food intake diminishes drastically, but circulating and stored carbohydrates and lipids are elevated. Gene transcripts of glucagon- and insulin-like peptides increase, and expression of several target genes of these peptides also change. Four key genes in innate immunity can be induced by infection in diapausing flies, and two of these, drosomycin and cecropin A1, are upregulated by diapause independently of infection. Diapausing flies display very low mortality, extended lifespan and decreased aging of the intestinal epithelium. Many phenotypes induced by diapause are reversed after one week of recovery from diapause conditions. Furthermore, mutant flies lacking specific insulin-like peptides (dilp5 and dilp2-3) display increased diapause incidence. Our study provides a first comprehensive characterization of reproductive diapause in D. melanogaster, and evidence that glucagon- and insulin-like signaling are among the key regulators of the altered physiology during this dormancy.

  8. The Sleeping Beauty: How Reproductive Diapause Affects Hormone Signaling, Metabolism, Immune Response and Somatic Maintenance in Drosophila melanogaster

    Science.gov (United States)

    Kubrak, Olga I.; Kučerová, Lucie; Theopold, Ulrich; Nässel, Dick R.

    2014-01-01

    Some organisms can adapt to seasonal and other environmental challenges by entering a state of dormancy, diapause. Thus, insects exposed to decreased temperature and short photoperiod enter a state of arrested development, lowered metabolism, and increased stress resistance. Drosophila melanogaster females can enter a shallow reproductive diapause in the adult stage, which drastically reduces organismal senescence, but little is known about the physiology and endocrinology associated with this dormancy, and the genes involved in its regulation. We induced diapause in D. melanogaster and monitored effects over 12 weeks on dynamics of ovary development, carbohydrate and lipid metabolism, as well as expression of genes involved in endocrine signaling, metabolism and innate immunity. During diapause food intake diminishes drastically, but circulating and stored carbohydrates and lipids are elevated. Gene transcripts of glucagon- and insulin-like peptides increase, and expression of several target genes of these peptides also change. Four key genes in innate immunity can be induced by infection in diapausing flies, and two of these, drosomycin and cecropin A1, are upregulated by diapause independently of infection. Diapausing flies display very low mortality, extended lifespan and decreased aging of the intestinal epithelium. Many phenotypes induced by diapause are reversed after one week of recovery from diapause conditions. Furthermore, mutant flies lacking specific insulin-like peptides (dilp5 and dilp2-3) display increased diapause incidence. Our study provides a first comprehensive characterization of reproductive diapause in D. melanogaster, and evidence that glucagon- and insulin-like signaling are among the key regulators of the altered physiology during this dormancy. PMID:25393614

  9. The Arabidopsis DSO/ABCG11 transporter affects cutin metabolism in reproductive organs and suberin in roots.

    Science.gov (United States)

    Panikashvili, David; Shi, Jian Xin; Bocobza, Samuel; Franke, Rochus Benni; Schreiber, Lukas; Aharoni, Asaph

    2010-05-01

    Apart from its significance in the protection against stress conditions, the cuticular cover is essential for proper development of the diverse surface structures formed on aerial plant organs. This layer mainly consists of a cutin matrix, embedded and overlaid with cuticular waxes. Following their biosynthesis in epidermal cells, cutin and waxes were suggested to be exported across the plasma membrane by ABCG-type transporters such as DSO/ABCG11 to the cell wall and further to extracellular matrix. Here, additional aspects of DSO/ABCG11 function were investigated, predominantly in reproductive organs, which were not revealed in the previous reports. This was facilitated by the generation of a transgenic DSO/ABCG11 silenced line (dso-4) that displayed relatively subtle morphological and chemical phenotypes. These included altered petal and silique morphology, fusion of seeds, and changes in levels of cutin monomers in flowers and siliques. The dso-4 phenotypes corresponded to the strong DSO/ABCG11 gene expression in the embryo epidermis as well as in the endosperm tissues of the developing seeds. Moreover, the DSO/ABCG11 protein displayed polar localization in the embryo protoderm. Transcriptome analysis of the dso-4 mutant leaves and stems showed that reduced DSO/ABCG11 activity suppressed the expression of a large number of cuticle-associated genes, implying that export of cuticular lipids from the plasma membrane is a rate-limiting step in cuticle metabolism. Surprisingly, root suberin composition of dso-4 was altered, as well as root expression of two suberin biosynthetic genes. Taken together, this study provides new insights into cutin and suberin metabolism and their role in reproductive organs and roots development.

  10. The FRIABLE1 gene product affects cell adhesion in Arabidopsis.

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    Lutz Neumetzler

    Full Text Available Cell adhesion in plants is mediated predominantly by pectins, a group of complex cell wall associated polysaccharides. An Arabidopsis mutant, friable1 (frb1, was identified through a screen of T-DNA insertion lines that exhibited defective cell adhesion. Interestingly, the frb1 plants displayed both cell and organ dissociations and also ectopic defects in organ separation. The FRB1 gene encodes a Golgi-localized, plant specific protein with only weak sequence similarities to known proteins (DUF246. Unlike other cell adhesion deficient mutants, frb1 mutants do not have reduced levels of adhesion related cell wall polymers, such as pectins. Instead, FRB1 affects the abundance of galactose- and arabinose-containing oligosaccharides in the Golgi. Furthermore, frb1 mutants displayed alteration in pectin methylesterification, cell wall associated extensins and xyloglucan microstructure. We propose that abnormal FRB1 action has pleiotropic consequences on wall architecture, affecting both the extensin and pectin matrices, with consequent changes to the biomechanical properties of the wall and middle lamella, thereby influencing cell-cell adhesion.

  11. Methylaplysinopsin and other marine natural products affecting neurotransmission.

    Science.gov (United States)

    Taylor, K M; Baird-Lambert, J A; Davis, P A; Spence, I

    1981-01-01

    Methylaplysinopsin is a novel marine natural product that, after oral administration, prevented the effects of tetrabenazine in mice and rats. Methylaplysinopsin was a short-acting inhibitor of monoamine oxidase activity with greatest potency when serotonin was the substrate studied. The brain concentration of serotonin in the mouse was increased by methylaplysinopsin over the same time course as monoamine oxidase inhibition ex vivo. Methylaplysinopsin was also a weak inhibitor of the neuronal uptake of [3H]serotonin and a potentiator of the K+-induced release of [3H]serotonin from prelabeled synaptosomes. The predicted potentiation of serotonergic neurotransmission was supported by initial neurophysiological studies in an identified serotonergic pathway in the central nervous system of Aplysia. Two other studies on the pharmacology of marine natural products are reviewed. The majority of polyhalogenated monoterpenes isolated from red algae had central nervous system depressant properties. The exception is plocamadiene A, which caused, in mice, a reversible spastic paresis lasting up to 72 hours after oral administration. The severe muscle spasm was antagonized by diazepam. The final study discussed is the effect of a variety of marine natural products on the synthesis, neuronal uptake, and metabolism of GABA. Their selectivity is discussed with regard to the effects on metabolic respiration, and the correlation of neurochemical and neurophysiological effects on these marine substances.

  12. Reproductive biology and nectar production of the Mexican endemic Psittacanthus auriculatus (Loranthaceae), a hummingbird-pollinated mistletoe.

    Science.gov (United States)

    Pérez-Crespo, M J; Ornelas, J F; Martén-Rodríguez, S; González-Rodríguez, A; Lara, C

    2016-01-01

    Many mistletoe species produce 'bird'-pollinated flowers; however, the reproductive biology of the majority of these species has not been studied. Psittacanthus auriculatus is a Mexican endemic mistletoe, most common in open, dry mesquite grassland. Knowledge of the reproductive biology of P. auriculatus is essential for understanding species formation and diversification of Psittacanthus mistletoes, but it is currently poorly understood. Thus, we studied floral biology and phenology, nectar production and breeding system and pollination of this species. The hermaphroditic red-pink flowers open from the middle to the tip and petals are curly, but remain partially fused forming a floral tube of ca. 20-mm long. Flowers are partially protandrous, produce large amounts of nectar, last 2 days, and stigma receptivity is highest during the second day. We recorded hummingbirds (Cynanthus latirostris, Hylocharis leucotis, Amazilia beryllina, A. violiceps, Calothorax lucifer, Archilochus colubris) and less commonly butterflies (Agraulis vanillae, Anteos clorinde, Papilio multicaudatus, Phocides urania, Phoebis sennae) as floral visitors. P. auriculatus flowers are self-compatible. However, this mistletoe is an obligate animal-pollinated species, as the sensitive stigma avoids self-pollination. Under natural conditions, reproductive success was higher than in manually selfed or cross-pollinated flowers, likely due to the traplining foraging behaviour of hummingbirds. We suggest that the apparent efficient foraging behaviour of hummingbirds maintains gene flow among P. auriculatus, promoting outcrossing.

  13. Blockage of the Neonatal Leptin Surge Affects the Gene Expression of Growth Factors, Glial Proteins, and Neuropeptides Involved in the Control of Metabolism and Reproduction in Peripubertal Male and Female Rats.

    Science.gov (United States)

    Mela, Virginia; Díaz, Francisca; Lopez-Rodriguez, Ana Belen; Vázquez, María Jesús; Gertler, Arieh; Argente, Jesús; Tena-Sempere, Manuel; Viveros, María-Paz; Chowen, Julie A

    2015-07-01

    Leptin (Lep) is important in the development of neuroendocrine circuits involved in metabolic control. Because both Lep and metabolism influence pubertal development, we hypothesized that early changes in Lep signaling could also modulate hypothalamic (HT) systems involved in reproduction. We previously demonstrated that a single injection of a Lep antagonist (Antag) on postnatal day (PND)9, coincident with the neonatal Lep peak, induced sexually dimorphic modifications in trophic factors and markers of cell turnover and neuronal maturation in the HT on PND13. Here, our aim was to investigate whether the alterations induced by Lep antagonism persist into puberty. Accordingly, male and female rats were treated with a pegylated super Lep Antag from PND5 to PND9 and killed just before the normal appearance of external signs of puberty (PND33 in females and PND43 in males). There was no effect on body weight, but in males food intake increased, subcutaneous adipose tissue decreased and HT neuropeptide Y and Agouti-related peptide mRNA levels were reduced, with no effect in females. In both sexes, the Antag increased HT mRNA levels of the kisspeptin receptor, G protein-coupled recepter 54 (Gpr54). Expression of the Lep receptor, trophic factors, and glial markers were differently affected in the HT of peripubertal males and females. Lep production in adipose tissue was decreased in Antag-treated rats of both sexes, with production of other cytokines being differentially regulated between sexes. In conclusion, in addition to the long-term effects on metabolism, changes in neonatal Lep levels modifies factors involved in reproduction that could possibly affect sexual maturation.

  14. Yucca brevifolia fruit production, predispersal seed predation, and fruit removal by rodents during two years of contrasting reproduction

    Science.gov (United States)

    Borchert, Mark I.; DeFalco, Lesley

    2016-01-01

    PREMISE OF THE STUDY: The distribution of Yucca brevifolia, a keystone species of the Mojave Desert, may contract with climate change, yet reproduction and dispersal are poorly understood. We tracked reproduction, seed predation, and fruit dispersal for two years and discuss whether Y. brevifolia is a masting species. METHODS: Fruit maturation, seed predation (larval yucca moths), and fruit dispersal (rodents) were monitored on a random sample of panicles during 2013 and 2014, which were years of high and low reproduction, respectively. Fates of fruits placed on the ground and in canopies were also tracked. Rodents were live-trapped to assess abundance and species composition. KEY RESULTS: In 2013, 66% of inflorescences produced fruit of which 53% escaped larval predation; 19.5% of seeds were destroyed in infested fruits. Total seed production was estimated to be >100 times greater in 2013 than 2014. One-third of the fruit crop fell to the ground and was removed by rodents over the course of 120 d. After ground fruits became scarce, rodents exploited canopy fruits. Rodent numbers were low in 2013, so fruits remained in canopies for 370 d. In 2014, fruit production was approximately 20% lower. Larvae infested the majority of fruits, and almost twice the number of seeds were damaged. Fruits were exploited by rodents within 65 d. CONCLUSIONS: High fertilization, prolific seed production, and low predispersal predation in 2013 suggests that pollinator attraction and satiation of seed predators influence masting in Y. brevifolia. Abundant, prolonged fruit availability to seed-dispersing rodents likely extends recruitment opportunities during mast years.

  15. Reproductive hacking

    Science.gov (United States)

    Dustin Rubinstein, C; Wolfner, Mariana F

    2014-01-01

    Seminal proteins are critical for reproductive success in all animals that have been studied. Although seminal proteins have been identified in many taxa, and female reproductive responses to receipt of these proteins have been documented in several, little is understood about the mechanisms by which seminal proteins affect female reproductive physiology. To explore this topic, we investigated how a Drosophila seminal protein, ovulin, increases ovulation rate in mated females. Ovulation is a relatively simple physiological process, with known female regulators: previous studies have shown that ovulation rate is promoted by the neuromodulator octopamine (OA) in D. melanogaster and other insects. We found that ovulin stimulates ovulation by increasing OA signaling in the female. This finding supports a model in which a male seminal protein acts through “hacking” a well-conserved, regulatory system females use to adjust reproductive output, rather than acting downstream of female mechanisms of control or in parallel pathways altogether. We also discuss similarities between 2 forms of intersexual control of behavior through chemical communication: seminal proteins and pheromones. PMID:25483253

  16. Factors Affecting the Production of Vietnamese Tones: A Study of American Learners

    Science.gov (United States)

    Nguyen, Hanh thi; Macken, Marlys A.

    2008-01-01

    This study investigates factors that affect the accuracy of tone production by American students of Vietnamese as a second language (L2). Nine hypotheses are examined, each of which isolates a factor expected to affect production accuracy: (a) task type, (b) the position of a tone in a clause, (c) discourse distance between a model provided by a…

  17. The entomopathogenic fungal endophytes Purpureocillium lilacinum (formerly Paecilomyces lilacinus) and Beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions.

    Science.gov (United States)

    Castillo Lopez, Diana; Zhu-Salzman, Keyan; Ek-Ramos, Maria Julissa; Sword, Gregory A

    2014-01-01

    The effects of two entomopathogenic fungal endophytes, Beauveria bassiana and Purpureocillium lilacinum (formerly Paecilomyces lilacinus), were assessed on the reproduction of cotton aphid, Aphis gossypii Glover (Homoptera:Aphididae), through in planta feeding trials. In replicate greenhouse and field trials, cotton plants (Gossypium hirsutum) were inoculated as seed treatments with two concentrations of B. bassiana or P. lilacinum conidia. Positive colonization of cotton by the endophytes was confirmed through potato dextrose agar (PDA) media plating and PCR analysis. Inoculation and colonization of cotton by either B. bassiana or P. lilacinum negatively affected aphid reproduction over periods of seven and 14 days in a series of greenhouse trials. Field trials were conducted in the summers of 2012 and 2013 in which cotton plants inoculated as seed treatments with B. bassiana and P. lilacinum were exposed to cotton aphids for 14 days. There was a significant overall effect of endophyte treatment on the number of cotton aphids per plant. Plants inoculated with B. bassiana had significantly lower numbers of aphids across both years. The number of aphids on plants inoculated with P. lilacinum exhibited a similar, but non-significant, reduction in numbers relative to control plants. We also tested the pathogenicity of both P. lilacinum and B. bassiana strains used in the experiments against cotton aphids in a survival experiment where 60% and 57% of treated aphids, respectively, died from infection over seven days versus 10% mortality among control insects. Our results demonstrate (i) the successful establishment of P. lilacinum and B. bassiana as endophytes in cotton via seed inoculation, (ii) subsequent negative effects of the presence of both target endophytes on cotton aphid reproduction using whole plant assays, and (iii) that the P. lilacinum strain used is both endophytic and pathogenic to cotton aphids. Our results illustrate the potential of using these

  18. The entomopathogenic fungal endophytes Purpureocillium lilacinum (formerly Paecilomyces lilacinus and Beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions.

    Directory of Open Access Journals (Sweden)

    Diana Castillo Lopez

    Full Text Available The effects of two entomopathogenic fungal endophytes, Beauveria bassiana and Purpureocillium lilacinum (formerly Paecilomyces lilacinus, were assessed on the reproduction of cotton aphid, Aphis gossypii Glover (Homoptera:Aphididae, through in planta feeding trials. In replicate greenhouse and field trials, cotton plants (Gossypium hirsutum were inoculated as seed treatments with two concentrations of B. bassiana or P. lilacinum conidia. Positive colonization of cotton by the endophytes was confirmed through potato dextrose agar (PDA media plating and PCR analysis. Inoculation and colonization of cotton by either B. bassiana or P. lilacinum negatively affected aphid reproduction over periods of seven and 14 days in a series of greenhouse trials. Field trials were conducted in the summers of 2012 and 2013 in which cotton plants inoculated as seed treatments with B. bassiana and P. lilacinum were exposed to cotton aphids for 14 days. There was a significant overall effect of endophyte treatment on the number of cotton aphids per plant. Plants inoculated with B. bassiana had significantly lower numbers of aphids across both years. The number of aphids on plants inoculated with P. lilacinum exhibited a similar, but non-significant, reduction in numbers relative to control plants. We also tested the pathogenicity of both P. lilacinum and B. bassiana strains used in the experiments against cotton aphids in a survival experiment where 60% and 57% of treated aphids, respectively, died from infection over seven days versus 10% mortality among control insects. Our results demonstrate (i the successful establishment of P. lilacinum and B. bassiana as endophytes in cotton via seed inoculation, (ii subsequent negative effects of the presence of both target endophytes on cotton aphid reproduction using whole plant assays, and (iii that the P. lilacinum strain used is both endophytic and pathogenic to cotton aphids. Our results illustrate the potential of

  19. How hollow melanosomes affect iridescent colour production in birds.

    Science.gov (United States)

    Eliason, Chad M; Bitton, Pierre-Paul; Shawkey, Matthew D

    2013-09-22

    Developmental constraints and trade-offs can limit diversity, but organisms have repeatedly evolved morphological innovations that overcome these limits by expanding the range and functionality of traits. Iridescent colours in birds are commonly produced by melanin-containing organelles (melanosomes) organized into nanostructured arrays within feather barbules. Variation in array type (e.g. multilayers and photonic crystals, PCs) is known to have remarkable effects on plumage colour, but the optical consequences of variation in melanosome shape remain poorly understood. Here, we used a combination of spectrophotometric, experimental and theoretical methods to test how melanosome hollowness--a morphological innovation largely restricted to birds--affects feather colour. Optical analyses of hexagonal close-packed arrays of hollow melanosomes in two species, wild turkeys (Meleagris gallopavo) and violet-backed starlings (Cinnyricinclus leucogaster), indicated that they function as two-dimensional PCs. Incorporation of a larger dataset and optical modelling showed that, compared with solid melanosomes, hollow melanosomes allow birds to produce distinct colours with the same energetically favourable, close-packed configurations. These data suggest that a morphological novelty has, at least in part, allowed birds to achieve their vast morphological and colour diversity.

  20. Productive and reproductive choices: report of a pilot survey of urban working women in Karachi.

    Science.gov (United States)

    Kazi, S; Sathar, Z A

    1986-01-01

    The primary objective of the pilot survey of urban working women in Karachi was to collect information on women's productive and reproductive choices within the context of the conditions in their household. A sample of working women in the formal sectors was selected through procurement of lists of personnel from factories, government offices, and so forth. The sample was not chosen systematically. The intention was not only to sample but to identify commonly found occupations among women. The sample of 110 working women included respondents between 19-50 years. 93 of these women were currently married, 6 were separated or divorced, and 11 were widowed. Most of the ethnic groups living in Karachi were represented. The sample includes 3 distinct socioeconomic groups: highly educated women employed in high status jobs with earnings above the average; women of lower middle class backgrounds who work in the formal sector but in lower-status occupations as compared with those of the professional group; and mostly uneducated women who work as low-status workers and whose incomes are meager. More than 70% of the women gave financial pressure as a reason for their entering the labor force. Women for whom the important reason for work was pursuit of a career or job satisfaction were highly educated and employed in high-status, remunerative jobs. Only 20 of the sample of 110 women worked for noneconomic reasons. Of 64 women working as factory workers, domestic servants, and other miscellaneous workers in the informal sector, there were only 2 women working out of choice. Women who worked before marriage comprised nearly 51% of the sample. There was a very strong positive association between work before marriage and level of education and occupation. A much larger percentage of educated women in professional jobs worked before marriage as compared with their uneducated or less educated counterparts. The majority of women in the static, low-income occupations did not work

  1. Solanum tuberosum and Lycopersicon esculentum Leaf Extracts and Single Metabolites Affect Development and Reproduction of Drosophila melanogaster.

    Directory of Open Access Journals (Sweden)

    Emanuela Ventrella

    Full Text Available Glycoalkaloids are secondary metabolites commonly found in Solanaceae plants. They have anti-bacterial, anti-fungal and insecticidal activities. In the present study we examine the effects of potato and tomato leaf extracts and their main components, the glycoalkaloids α-solanine, α-chaconine and α-tomatine, on development and reproduction of Drosophila melanogaster wild-type flies at different stages. Parental generation was exposed to five different concentrations of tested substances. The effects were examined also on the next, non-exposed generation. In the first (exposed generation, addition of each extract reduced the number of organisms reaching the pupal and imaginal stages. Parent insects exposed to extracts and metabolites individually applied showed faster development. However, the effect was weaker in case of single metabolites than in case of exposure to extracts. An increase of developmental rate was also observed in the next, non-exposed generation. The imagoes of both generations exposed to extracts and pure metabolites showed some anomalies in body size and malformations, such as deformed wings and abdomens, smaller black abdominal zone. Our results further support the current idea that Solanaceae can be an impressive source of molecules, which could efficaciously be used in crop protection, as natural extract or in formulation of single pure metabolites in sustainable agriculture.

  2. Solanum tuberosum and Lycopersicon esculentum Leaf Extracts and Single Metabolites Affect Development and Reproduction of Drosophila melanogaster

    Science.gov (United States)

    Ventrella, Emanuela; Adamski, Zbigniew; Chudzińska, Ewa; Miądowicz-Kobielska, Mariola; Marciniak, Paweł; Büyükgüzel, Ender; Büyükgüzel, Kemal; Erdem, Meltem; Falabella, Patrizia; Scrano, Laura; Bufo, Sabino Aurelio

    2016-01-01

    Glycoalkaloids are secondary metabolites commonly found in Solanaceae plants. They have anti-bacterial, anti-fungal and insecticidal activities. In the present study we examine the effects of potato and tomato leaf extracts and their main components, the glycoalkaloids α-solanine, α-chaconine and α-tomatine, on development and reproduction of Drosophila melanogaster wild-type flies at different stages. Parental generation was exposed to five different concentrations of tested substances. The effects were examined also on the next, non-exposed generation. In the first (exposed) generation, addition of each extract reduced the number of organisms reaching the pupal and imaginal stages. Parent insects exposed to extracts and metabolites individually applied showed faster development. However, the effect was weaker in case of single metabolites than in case of exposure to extracts. An increase of developmental rate was also observed in the next, non-exposed generation. The imagoes of both generations exposed to extracts and pure metabolites showed some anomalies in body size and malformations, such as deformed wings and abdomens, smaller black abdominal zone. Our results further support the current idea that Solanaceae can be an impressive source of molecules, which could efficaciously be used in crop protection, as natural extract or in formulation of single pure metabolites in sustainable agriculture. PMID:27213896

  3. OVARIAN HORMONE PRODUCTION AFFECTED BY AMYGDALIN ADDITION IN VITRO

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    Marek Halenár

    2015-02-01

    Full Text Available Amygdalin, a natural substance, is a cyanogenic glycoside occurring in the seeds of apricots and bitter almonds. It is a controversial anti-tumor compound that has been used as an alternative cancer drug for many years. Amygdalin is composed of two molecules of glucose, one of benzaldehyde, which induces an analgesic action, and one of hydrocyanic acid, which is an anti-neoplastic compound. This in vitro study was performed to evaluate the possible impact of amygdalin (1, 10, 100, 1000, 10 000 μg/mL on the secretory activity of granulosa cells (GCs from porcine cyclic ovaries. The release of progesterone and estradiol-17β by GCs were evaluated by ELISA. In our study, the noticeable changes in estradiol-17β release by ovarian GCs were determined after the amygdalin addition. Amygdalin, at the highest dose (10 000 μg/mL, significantly (P≤0.05 stimulated the release of estradiol-17β by GCs, in comparison to the untreated control cells. On the contrary, no significant (P≥0.05 changes in the progesterone release by GCs caused by amygdalin addition were observed. In conclusion, obtained results showed that the amygdalin application (various doses to ovarian GCs caused a dose-dependent stimulation of the estradiol-17β release, but not progesterone, and its possible modulatory impact on the steroid production in porcine ovaries.

  4. Reproductive physiology

    Science.gov (United States)

    Gee, G.F.; Russman, S.E.; Ellis, David H.; Gee, George F.; Mirande, Claire M.

    1996-01-01

    Conclusions: Although the general pattern of avian physiology applies to cranes, we have identified many physiological mechanisms (e.g., effects of disturbance) that need further study. Studies with cranes are expensive compared to those done with domestic fowl because of the crane's larger size, low reproductive rate, and delayed sexual maturity. To summarize, the crane reproductive system is composed of physiological and anatomical elements whose function is controlled by an integrated neural-endocrine system. Males generally produce semen at a younger age than when females lay eggs. Eggs are laid in clutches of two (1 to 3), and females will lay additional clutches if the preceding clutches are removed. Both sexes build nests and incubate the eggs. Molt begins during incubation and body molt may be completed annually in breeding pairs. However, remiges are replaced sequentially over 2 to 3 years, or abruptly every 2 to 3 years in other species. Most immature birds replace their juvenal remiges over a 2 to 3 year period. Stress interferes with reproduction in cranes by reducing egg production or terminating the reproductive effort. In other birds, stress elevates corticosterone levels and decreases LHRH release. We know little about the physiological response of cranes to stress.

  5. The nuclear hormone receptor coactivator NRC is a pleiotropic modulator affecting growth, development, apoptosis, reproduction, and wound repair.

    Science.gov (United States)

    Mahajan, Muktar A; Das, Sharmistha; Zhu, Hong; Tomic-Canic, Marjana; Samuels, Herbert H

    2004-06-01

    Nuclear hormone receptor coregulator (NRC) is a 2,063-amino-acid coregulator of nuclear hormone receptors and other transcription factors (e.g., c-Fos, c-Jun, and NF-kappaB). We and others have generated C57BL/6-129S6 hybrid (C57/129) NRC(+/-) mice that appear outwardly normal and grow and reproduce. In contrast, homozygous deletion of the NRC gene is embryonic lethal. NRC(-/-) embryos are always smaller than NRC(+/+) embryos, and NRC(-/-) embryos die between 8.5 and 12.5 days postcoitus (dpc), suggesting that NRC has a pleotrophic effect on growth. To study this, we derived mouse embryonic fibroblasts (MEFs) from 12.5-dpc embryos, which revealed that NRC(-/-) MEFs exhibit a high rate of apoptosis. Furthermore, a small interfering RNA that targets mouse NRC leads to enhanced apoptosis of wild-type MEFs. The finding that C57/129 NRC(+/-) mice exhibit no apparent phenotype prompted us to develop 129S6 NRC(+/-) mice, since the phenotype(s) of certain gene deletions may be strain dependent. In contrast with C57/129 NRC(+/-) females, 20% of 129S6 NRC(+/-) females are infertile while 80% are hypofertile. The 129S6 NRC(+/-) males produce offspring when crossed with wild-type 129S6 females, although fertility is reduced. The 129S6 NRC(+/-) mice tend to be stunted in their growth compared with their wild-type littermates and exhibit increased postnatal mortality. Lastly, both C57/129 NRC(+/-) and 129S6 NRC(+/-) mice exhibit a spontaneous wound healing defect, indicating that NRC plays an important role in that process. Our findings reveal that NRC is a coregulator that controls many cellular and physiologic processes ranging from growth and development to reproduction and wound repair.

  6. THE EFFECT OF BREED ON REPRODUCTIVE PERFORMANCE IN COMMODITY RABBIT PRODUCTION

    OpenAIRE

    Jadwiga TOPCZEWSKA; Anna ROGOWSKA; Leszek GACEK

    2013-01-01

    The aim of this study was to assess the reproductive rates among different breeds, mostly used in commodity farm conditions. The studies were obtained at fertility level from 6.27 animals in the Californian breed to 8.49 animals in Popielno White. High rate of failures during rearing was observed in the Californian breed. Litter weight was dependent on the number of births and young rearing, lactation and care of female. Kitten weight at 35 day of age ranged from 603.21g (Alaskan) to 736.10g ...

  7. DOMINANT FACTORS AFFECTING SEAWEED (Gracilaria verrucosa PRODUCTION IN ACID SULFATE SOILS-AFFECTED PONDS OF LUWU REGENCY, INDONESIA

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    Akhmad Mustafa

    2010-12-01

    Full Text Available Most of brackish water ponds used for seaweed (Gracilaria verrucosa culture in Luwu Regency, South Sulawesi, Indonesia are constructed on acid sulfate soil. Despite this inevitable condition, opportunities remain open to increase the seaweed production. The research was conducted to study the dominant factors that affect the seaweed production in ASS-affected ponds of Luwu Regency. As a dependent variable in this research is seaweed production. Independent variables were grouped into: (a farmer status factor, consisting of 9 variables; (b pond condition factor, consisting of 8 variables; (c pond management factor, consisting of 29 variables; (d soil quality factor, consisting of 17 variables and (e water quality factor, consisting of 11 variables. Multiple regression with dummy variable was used to analyze the data in prediction dependent variable. Results show that the average of seaweed production in ASS-affected pond of Luwu Regency is 11,000 kg dry/ha/year. Seaweed production can be increased through: (a decreasing dosage of urea and KCl and increasing dosage and frequency of fertilizer containing phosphate; (b increasing water depth in the pond and decreasing percentage of water exchange,(c conducting remediation to increase the soil pH and decreasing the concentration of Fe in the water, (d increasing stocking density of milkfish to decrease the epiphyte population and (e increasing the frequency of the farmer to attend trainings.

  8. Does the Unemployement Benefit Institution Affect the Productivity of Workers? Evidence from a Field Experiment

    NARCIS (Netherlands)

    Blanco, M.; Dalton, P.S.; Vargas, J.F.

    2013-01-01

    Abstract: We investigate whether and how the type of unemployment bene t institution affects productivity. We designed a field experiment to compare workers' productivity under a welfare system, where the unemployed receive an unconditional monetary transfer, with their productivity under a workfare

  9. Morphological traits, reproductive and productive performances of Casertana pigs reared outdoors

    Directory of Open Access Journals (Sweden)

    Donato Matassino

    2010-01-01

    Full Text Available Casertana is an Italian autochthonous pig breed. In the past, the breed was common in Campania and the surrounding area in South Italy because it was valued for its productive performance, particularly regarding the fattening tendency, but in recent decades, autochthonous swine have been replaced by cosmopolitan lean-type pigs and the Casertana breed also experienced a dramatic reduction in numbers. Concern over the preservation of genetic resources and the increased demand for regional food suggest a new way of maintaining the breed. Unfortunately, few recent data on the morpho- logical as well as on the productive traits are available. To ascertain the characteristics of the Casertana breed a pro- ductive trial was performed by comparing Casertana and Large White pure breed and their cross. Sixteen females and 6 males of pure breed Casertana and 18 females and 2 males of pure breed Italian Large White were mated twice to pro- duce Casertana, Large White and Casertana x Large White piglets. The average number of piglets born was significant- ly lower in Casertana gilts, but no difference in the number of weaned piglets among genetic types was observed. Sixty- eight animals of the three genetic types were reared outdoors and received two distinct diets differing in energy content. Pigs were weighted individually every month to calculate average daily gain. Morphological traits of Casertana pigs were also recorded. A total of 54 animals (21 Casertana at 151 kg live weight, 15 crossbreed at 157 kg live weight and 18 Large White at 179 kg live weight were slaughtered and data on carcasses were collected. Average daily weight gain differed markedly (P< 0.05 among genetic types (450; 552; 695 g/d, respectively for Casertana; Casertana x Large White; Large White and consequently the slaughter weight also differed. Casertana showed higher dressing percentages that the Italian Large White (P< 0.05. Backfat thickness was also clearly affected by genetic

  10. Does warming affect growth rate and biomass production of shrubs in the High Arctic?

    DEFF Research Database (Denmark)

    Campioli, Matteo; Schmidt, Niels Martin; Albert, Kristian Rost

    2013-01-01

    Few studies have assessed directly the impact of warming on plant growth and biomass production in the High Arctic. Here, we aimed to investigate the impact of 7 years of warming (open greenhouses) on the aboveground relative growth rate (RGR) of Cassiope tetragona and Salix arctica in North......-Eastern Greenland. RGR was assessed for apical (leaves, stem, reproductive organs) and lateral meristems (secondary growth of stem and branches) and accompanied by measures of gross ecosystem production (GEP), branching and tissue carbon (C) concentration. Measurements were based on harvest and biometric methods...... limits the growth of Cassiope but not that of Salix in North-Eastern Greenland. Summer warming thus has the potential to stimulate biomass production in the High Arctic but major species-specific differences are expected....

  11. Emerging issues and methodological advances in fisheries reproductive biology

    DEFF Research Database (Denmark)

    Lowerre-Barbieri, Susan K.; Brown-Peterson, Nancy J.; Murua, Hilario;

    2011-01-01

    Although incorporating detailed reproductive data into all stock assessments is not a practical goal, the need to understand how reproductive biology affects population productivity is being increasingly recognized.More research focused on reproductive biology—coupled with a shift towards...... development, which is most accurately evaluated with histology. This special section of Marine and Coastal Fisheries contains contributions from a workshop on the gonadal histology of fishes that was held in Cadiz, Spain, during June 2009. These papers cover a wide range of species and reproductive topics...... a resilience perspective in fisheries science—is resulting in challenges to many long-held assumptions; the emergence of important new issues; and identification of the need to improve data and methods used in reproductive studies. Typically, data for reproductive studies are based on an assessment of gonadal...

  12. Advances in reproductive biotechnologies.

    Science.gov (United States)

    Choudhary, K K; Kavya, K M; Jerome, A; Sharma, R K

    2016-04-01

    In recent times, reproductive biotechnologies have emerged and started to replace the conventional techniques. It is noteworthy that for sustained livestock productivity, it is imperative to start using these techniques for facing the increasing challenges for productivity, reproduction and health with impending environment conditions. These recent biotechniques, both in male and female, have revolutionized and opened avenues for studying and manipulating the reproductive process both in vitro and in vivo in various livestock species for improving tis efficiency. This review attempts to highlight pros and cons, on the recent developments in reproductive biotechnologies, both in male and female in livestock species.

  13. The Means of (Re-Production: Expertise, Open Tools, Standards and Communication

    Directory of Open Access Journals (Sweden)

    Martin Paul Eve

    2014-02-01

    Full Text Available This article examines the current difficulties faced in penetrating the world of scholarly communication technology. While there have been large strides forward in the disintermediation of digital publishing expertise—most notably by the Public Knowledge Project—a substantial number of barriers remain. This paper examines a case study in terms of scholarly typesetting and the Journal Article Tag Suite (JATS standard before moving to suggest three potential solutions: (1 The formation of open, non-commercial and inclusive (but structured organizations dedicated to the group exploration and standardisation of scholarly publishing technology; (2 The collective authoring of as much technological and process documentation on scholarly publishing as is possible; (3 The modularisation of platforms and agreement on standards of interoperability. Only through such measures is it possible for researchers to reclaim the means of (reproduction, for the remaining barriers are not difficult to understand, merely hard to discover.

  14. Brokerage in Cross-Border Mobility: Social Mechanisms and the (ReProduction of Social Inequalities

    Directory of Open Access Journals (Sweden)

    Thomas Faist

    2014-12-01

    Full Text Available Brokerage is an essential yet understudied function in social life. In one of the classics in the field of sociology, Georg Simmel differentiated three types of the “third” which help to analyse brokerage: the disinterested mediator or arbitrator, tertius gaudens and divide et impera. Studies that conceptualise traffickers and smugglers as brokers are extremely rare. Scholars lack a typology which can serve as a basis for comparative research. To advance scholarship on brokerage this article seeks to develop a conceptual-typological matrix by setting out to explore three questions: Why does brokerage exist? What kind of social mechanism is brokerage? What are the implications of brokerage for social inequalities and equalities? The analysis concludes with the consequences of different types of brokerage for the (reproduction of social inequalities.

  15. Growth characteristics, reproductive performance, and evaluation of their associative relationships in Brangus cattle managed in a Chihuahuan Desert production system1.

    Science.gov (United States)

    Luna-Nevarez, P; Bailey, D W; Bailey, C C; VanLeeuwen, D M; Enns, R M; Silver, G A; DeAtley, K L; Thomas, M G

    2010-05-01

    Balancing growth and reproductive performance in beef cattle managed in desert environments is challenging. Our objectives were to 1) evaluate trends in growth and reproductive traits, and 2) assess associative relationships between growth characteristics and reproductive performance in a Brangus herd managed in a Chihuahuan Desert production system from 1972 to 2006. Data were from bull (n = 597) and heifer calves (n = 585; 1988 to 2006) and cows (n = 525; repeated records of cows, n = 2,611; 1972 to 2006). Variables describing the growth curve of each cow were estimated using a nonlinear logistic function (each cow needed 6 yr of data). Mixed-effect models and logistic regression were used to analyze trends across years in growth and reproductive traits (both continuous and categorical). For continuous traits of calves, a slight cubic response (P performance suggested that fertility improved.

  16. Types of embodiment in design: the embodied foundations of meaning and affect in product design

    NARCIS (Netherlands)

    Rompay, van Thomas J.L.; Ludden, Geke D.S.

    2015-01-01

    Since the introduction of the embodied cognition framework in cognitive and social psychology, numerous studies have demonstrated links between everyday bodily experiences and product evaluations involving meaning portrayal and affect. To date, however, no comprehensive framework for understanding e

  17. Reproductive efficiency and herd demography of Nguni cattle in village-owned and group-owned enterprises under low-input communal production systems.

    Science.gov (United States)

    Tada, Obert; Muchenje, Voster; Dzama, Kennedy

    2013-08-01

    The objective of the study was to determine the herd demography and reproductive efficiency of the Nguni cattle in village-owned and group-owned enterprises under low-input communal production systems. Data on husbandry practices, reason of cattle entry/exist, herd structure, bulling rates, breeding females, age at first calving and calving interval were obtained from 22 village-owned and 19 group-owned enterprises in a cross-sectional survey of an ecologically controlled low-input cattle production system. Descriptive statistics and chi-square tests of association were computed on enterprise ownership patterns, husbandry practices and herd demography. An AN(C)OVA was used to determine significant factors affecting herd structure, mortality, age at first calving and calving interval in the enterprises. Village-owned enterprises had higher (p 0.05). Significant differences were observed on the calving interval (p bulling rate was higher in village-owned enterprises, while the proportion of breeding females was higher in group-owned enterprises. Farmers with a college education had Nguni animals with the shortest calving interval. It was concluded that group-owned enterprises had significantly better calving intervals, mortality rates and overall herd structure than village-owned enterprises.

  18. Efficient production of bioactive metabolites from Antrodia camphorata ATCC 200183 by asexual reproduction-based repeated batch fermentation.

    Science.gov (United States)

    Li, Hua-Xiang; Lu, Zhen-Ming; Geng, Yan; Gong, Jin-Song; Zhang, Xiao-Juan; Shi, Jin-Song; Xu, Zheng-Hong; Ma, Yan-He

    2015-10-01

    Large-scale submerged fermentation (SmF) of Antrodia camphorata (A. camphorata) usually encounters challenges including tedious preparation of mycelial inoculum, long fermentation period (10-14 d), and poor repeatability. Here we developed an asexual reproduction-based repeated batch fermentation (RBF) process for bioactive metabolites production by A. camphorata ATCC 200183. Compared with traditional batch fermentation, production time was shortened to 58 d from 80 d (overall time for eight cycles) using the RBF process established in this study, and accordingly, the productivities of bioactive metabolites (including antrodins) were improved by 40-60%. Kinetic parameters (α is 2.1-18.7 times as β) indicated that the cell growth was the major contribution for bioactive metabolites production. The RBF shows excellent batch-repeatability (Pearson correlation coefficient of 0.998±0.001), together with advantages of energy-efficient, low cost, and labor-saving, RBF process can be implemented to SmF by other filamentous fungi.

  19. Sublethal gamma irradiation affects reproductive impairment and elevates antioxidant enzyme and DNA repair activities in the monogonont rotifer Brachionus koreanus

    Energy Technology Data Exchange (ETDEWEB)

    Han, Jeonghoon; Won, Eun-Ji [Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of); Kim, Il-Chan; Yim, Joung Han [Division of Life Sciences, Korea Polar Research Institute, Incheon 406-840 (Korea, Republic of); Lee, Su-Jae [Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791 (Korea, Republic of); Lee, Jae-Seong, E-mail: jslee2@skku.edu [Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of)

    2014-10-15

    Highlights: • No mortality within 96 h even at a high intensity (1200 Gy). • A reduced fecundity of Brachionus koreanus at over 150 Gy with a decrease in lifespan. • Dose-dependent ROS increase with GST enzyme activity at sub-lethal doses. • Significant impact on life table parameters, particularly fecundity. • Significant up-regulation of DNA repair-associated genes at sublethal doses. - Abstract: To examine the effects of gamma radiation on marine organisms, we irradiated several doses of gamma ray to the microzooplankton Brachionus koreanus, and measured in vivo and in vitro endpoints including the survival rate, lifespan, fecundity, population growth, gamma ray-induced oxidative stress, and modulated patterns of enzyme activities and gene expressions after DNA damage. After gamma radiation, no individuals showed any mortality within 96 h even at a high intensity (1200 Gy). However, a reduced fecundity (e.g. cumulated number of offspring) of B. koreanus at over 150 Gy was observed along with a slight decrease in lifespan. At 150 Gy and 200 Gy, the reduced fecundity of the rotifers led to a significant decrease in population growth, although in the second generation the population growth pattern was not affected even at 200 Gy when compared to the control group. At sub-lethal doses, reactive oxygen species (ROS) levels dose-dependently increased with GST enzyme activity. In addition, up-regulations of the antioxidant and chaperoning genes in response to gamma radiation were able to recover cellular damages, and life table parameters were significantly influenced, particularly with regard to fecundity. DNA repair-associated genes showed significantly up-regulated expression patterns in response to sublethal doses (150 and 200 Gy), as shown in the expression of the gamma-irradiated B. koreanus p53 gene, suggesting that these sublethal doses were not significantly fatal to B. koreanus but induced DNA damages leading to a decrease of the population size.

  20. Reproductive and Productive Performance of Iraqi Buffaloes as Influenced by Pre-Mating and Pre-Calving Concentrate Supplementation

    Directory of Open Access Journals (Sweden)

    Talal Anwer Abdulkareem*, Sawsan Ali Al-Sharifi, Sajeda Mahdi Eidan and R.G. Sasser1

    2012-06-01

    Full Text Available The objective of the present study was to investigate the influence of pre-mating and pre-calving concentrate supplementation of Iraqi buffaloes on some of the reproductive (estrus, mating, pregnancy and calving rates and productive (daily milk yield and calves birth weight traits. This study was carried out in 4 Iraqi South-central governorates using 596 pre-mating and 628 pregnant buffaloes (during the last two months of gestation. Pre-mating buffaloes were divided randomly into 496 concentrate-supplemented buffaloes (Flushing and 100 control ones. Additionally, pregnant buffaloes were also divided into 528 concentrate- supplemented buffaloes (Steaming up and 100 controls. Each buffalo within the flushing and steaming up groups were fed daily on 7 Kg of concentrate diet (13% crude protein and 1.70 Mcal of net energy for 60 days. The control buffaloes were nourished only on low-quality roughages of the area and wheat bran. Higher estrus (+15%, P<0.01, pregnancy (+23.8%, P<0.05 and calving rates (+30.8%, P<0.01 were observed in concentrate-supplemented buffaloes as compared with controls. An obvious increase in (P<0.05 calving rate (+14.7%, daily milk yield (+44.8% and calf birth weight (+25.6% were noted in steaming up buffaloes in comparison with control buffaloes. Results indicated that improvement in feeding schedule of Iraqi buffaloes during pre-mating and late gestation periods enhanced the reproductive performance and increased milk production of subsequent lactation and calf birth weight. These improvements increased owner income ($174=209,000 Iraqi dinar /buffalo from the sale of meat and milk.

  1. Productivity affects the density-body mass relationship of soil fauna communities

    NARCIS (Netherlands)

    Comor, V.N.R.; Thakur, M.P.; Berg, M.P.; Bie, de S.; Prins, H.H.T.; Langevelde, van F.

    2014-01-01

    The productivity of ecosystems and their disturbance regime affect the structure of animal communities. However, it is not clear which trophic levels benefit the most from higher productivity or are the most impacted by disturbance. The density-body mass (DBM) relationship has been shown to reflect

  2. Porcine reproductive and respiratory syndrome virus infection triggers HMGB1 release to promote inflammatory cytokine production.

    Science.gov (United States)

    Duan, Erzhen; Wang, Dang; Luo, Rui; Luo, Jingyi; Gao, Li; Chen, Huanchun; Fang, Liurong; Xiao, Shaobo

    2014-11-01

    The high mobility group box 1 (HMGB1) protein is an endogenous damage-associated molecular pattern (DAMP) molecule involved in the pathogenesis of various infectious agents. Based on meta-analysis of all publicly available microarray datasets, HMGB1 has recently been proposed as the most significant immune modulator during the porcine response to porcine reproductive and respiratory syndrome virus (PRRSV) infection. However, the function of HMGB1 in PRRSV pathogenesis is unclear. In this study, we found that PRRSV infection triggers the translocation of HMGB1 from the nucleus to the extracellular milieu in MARC-145 cells and porcine alveolar macrophages. Although HMGB1 has no effect on PRRSV replication, HMGB1 promotes PRRSV-induced NF-κB activation and subsequent expression of inflammatory cytokines through receptors RAGE, TLR2 and TLR4. Our findings show that HMGB1 release, triggered by PRRSV infection, enhances the efficiency of virus-induced inflammatory responses, thereby providing new insights into the pathogenesis of PRRSV infection.

  3. Resource effects on solitary bee reproduction in a managed crop pollination system

    Science.gov (United States)

    The number of solitary bees (Megachile rotundata) released for pollination in a managed system (Medicago sativa seed production) and the number of flowers available for brood provisioning may affect reproduction through maternal resource allocation and investment. Overwhelming, limited, or adequate...

  4. Study on affecting factors of collaborative product development based on collaboration hierarchy model

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiaodong; LI Yingzi; ZHANG Zhiqiang

    2007-01-01

    Aiming at the levels of collaborative degree in web-based product development,a collaboration hierarchy model of this product development is developed in this paper.Based on the model,the affecting factors on collaboration levels are analyzed systematically from many aspects,such as technology,organization and business.A gap analysis method is studied in detail,and is applied in a real project.The application shows that it can solve the diverse problems of collaborative product development effectively,and help enterprises find out the critical factors that affect the collaboration.

  5. Does the Unemployement Benefit Institution Affect the Productivity of Workers? Evidence from a Field Experiment

    OpenAIRE

    2013-01-01

    Abstract: We investigate whether and how the type of unemployment bene t institution affects productivity. We designed a field experiment to compare workers' productivity under a welfare system, where the unemployed receive an unconditional monetary transfer, with their productivity under a workfare system, where the transfer is received conditional on the unemployed spending some time on ancillary activities. First, we fi nd that having an unemployment bene fit institution, regardless of whe...

  6. Productive Love Promotion Via Affective Technology: An Approach Based On Social Psychology And Philosophy

    Directory of Open Access Journals (Sweden)

    Ramon Solves Pujol

    2010-01-01

    Full Text Available This paper proposes the use of social psychological and philosophical foundations for designing affective technology that promotes the experience of love. The adopted theoretical basis is the concept of productive love, which is heavily based on Enrich Fromm but also includes theories and scientific findings of numerous psychoanalysts, social psychologists, and philosophers. We conducted a review of the theory about the nature of love and found that social psychological and philosophical approaches differ regarding peoples' understandings. The findings were used to elaborate eight principles of productive love. Based on these principles, we derived criteria for designing affective technology when the objective is to promote productive love. We reviewed the existent studies on affective technologies and implemented the criteria into a system design, the Pictures' Call. A prototype of the system was pretested to illustrate how productive love technology could be based on established criteria.

  7. Selective Reproduction

    DEFF Research Database (Denmark)

    Svendsen, Mette N.

    2015-01-01

    This article employs a multi-species perspective in investigating how life's worth is negotiated in the field of neonatology in Denmark. It does so by comparing decision-making processes about human infants in the Danish neonatal intensive care unit with those associated with piglets who serve as...... as expectations within linear or predictive time frames are key markers in both sites. Exploring selective reproductive processes across human infants and research piglets can help us uncover aspects of the cultural production of viability that we would not otherwise see or acknowledge....

  8. Factors affecting industrial wood, material production yield in Turkey’s natural beech forests

    Directory of Open Access Journals (Sweden)

    Atilla Atik

    2014-07-01

    Full Text Available The objectives of the present study are to determine the most important factors affecting industrial wood material production yield in natural oriental beech forests in Turkey using a multifaceted approach and to help entrepreneurs consider these factors to develop more sensitive and realistic production plans. In Günye Forest Management in Bartın province of the West Black Sea Region of Turkey, 41 production units were chosen as the study area. The 1277 ha study area was included in the 2007 and 2010 production management plan. The general state of the stand, natural stand structure, and production methods and tools are the factors thought most strongly affect industrial wood material production yield; 26 variables representing these factors were evaluated in the study. Through multidimensional statistical analyses, including main components, factor and regression  analysis, we found that the most important factors affecting production yield were fertility, aspect of land, skidding method, stand structure, skidding distance, growing stock, transportation and harmful abiotic factors. Production units were divided into three groups based on yield rates and the 26 variables, using discriminate analysis. From the results of the study, a sample model can be developed to help forest managers predict and plan annual industrial wood production more sensitively and realistically.

  9. Demographic consequences of greater clonal than sexual reproduction in Dicentra canadensis.

    Science.gov (United States)

    Lin, Chia-Hua; Miriti, Maria N; Goodell, Karen

    2016-06-01

    Clonality is a widespread life history trait in flowering plants that may be essential for population persistence, especially in environments where sexual reproduction is unpredictable. Frequent clonal reproduction, however, could hinder sexual reproduction by spatially aggregating ramets that compete with seedlings and reduce inter-genet pollination. Nevertheless, the role of clonality in relation to variable sexual reproduction in population dynamics is often overlooked. We combined population matrix models and pollination experiments to compare the demographic contributions of clonal and sexual reproduction in three Dicentra canadensis populations, one in a well-forested landscape and two in isolated forest remnants. We constructed stage-based transition matrices from 3 years of census data to evaluate annual population growth rates, λ. We used loop analysis to evaluate the relative contribution of different reproductive pathways to λ. Despite strong temporal and spatial variation in seed set, populations generally showed stable growth rates. Although we detected some pollen limitation of seed set, manipulative pollination treatments did not affect population growth rates. Clonal reproduction contributed significantly more than sexual reproduction to population growth in the forest remnants. Only at the well-forested site did sexual reproduction contribute as much as clonal reproduction to population growth. Flowering plants were more likely to transition to a smaller size class with reduced reproductive potential in the following year than similarly sized nonflowering plants, suggesting energy trade-offs between sexual and clonal reproduction at the individual level. Seed production had negligible effects on growth and tuber production of individual plants. Our results demonstrate that clonal reproduction is vital for population persistence in a system where sexual reproduction is unpredictable. The bias toward clonality may be driven by low fitness returns

  10. Reproductive Performance of Native Pulawska and High Productivity Polish Landrace Sows in the Context of Stress During the Period of Early Pregnancy.

    Science.gov (United States)

    Babicz, M; Szyndler-Nędza, M; Skrzypczak, E; Kasprzyk, A

    2016-02-01

    Experiments revealed that it is necessary to get rid of genotypes RYR1C/T and RYR1T/T because of problems with meat quality and reproduction of gilts and sows. This phenomenon, however, is of individual nature and is characterized by high variability. The aim of the study was to analyse the influence of stressogenic factors in native Puławska and high productivity Polish Landrace breed sows during the period of low pregnancy on reproductive performance and maternal behaviour. Sows in the 2nd reproduction cycle were assigned into groups of five animals as follows: control (C) of RYR1 C/C genotype and experimental (E) of RYR1 C/T genotype. In all, 30 Puławska breed sows and 30 PL breed sows were analysed. During the early stage of pregnancy (days 42-84), experimental groups were subjected to the action of stressogenic factors: elevated temperature, noisy and rough treatment of sows by service personnel and immobilization stress. The effect of stressogenic factors was referred to the reproductive performance of sows. Puławska and PL breed sows from the experimental groups were subjected to the evaluation of maternal traits on the basis of perinatal behaviour determined with the help of an ethogram. Analysis of the results of reproduction and the behaviour of the sows confirmed the higher resistance to stressogenic factors of the Puławska breed. The performed analysis of reproduction and behaviour confirmed that heterozygotic genotypes in the RYR1 locus exhibited less advantageous reproduction, which corroborates the hypothesis about a negative impact of the T allele on swine productivity.

  11. Is intensification of reproduction rhythm sustainable in an organic sheep production system? A 4-year interdisciplinary study.

    Science.gov (United States)

    Benoit, M; Tournadre, H; Dulphy, J P; Laignel, G; Prache, S; Cabaret, J

    2009-05-01

    A 4-year interdisciplinary study was performed to compare two organically managed sheep production systems, using 118 ewes and 24 ha each. The systems differed in the ewes' reproduction rhythm: one lambing per ewe per year (1L/1Y), with the aim of balancing feed self-sufficiency and lamb marketing periods v. three lambings over 2 years (3L/2Y), with the aim of maximising ewes' productivity. The sustainability was evaluated through ewes' reproductive performance, lamb growth rate, carcass characteristics and quality, animal health, forage and feed self-sufficiency, soil mineral balance and gross margin. General animal health was assessed by recording ewe and lamb mortality and putative cause of death. Nematode digestive-tract strongyles parasitism was studied using faecal egg counts and necropsies. Carcass quality was assessed by recording carcass weight, conformation and fatness, and colour and firmness of subcutaneous fat. Thirty-three percent of 3L/2Y ewes lambed twice a year against 4% of 1L/1Y. Mean ewe productivity was 161.3% and 151.0% in 3L/2Y and 1L/1Y, respectively, and it was more variable between years in 3L/2Y. Average concentrate feed consumption and the corresponding cost per ewe were higher in 3L/2Y than in 1L/1Y (156 v. 121 kg, and €49.5 v. €39.3, respectively). Finally, average gross margin was lower in 3L/2Y than in 1L/1Y (€59 v. €65 per ewe, respectively). Even in year 2002, when ewe productivity was highest in 3L/2Y (193%), gross margin was not different between systems (€90 v. €86 per ewe in 3L/2Y and 1L/1Y, respectively), because of higher concentrate costs in 3L/2Y. The 3L/2Y animals presented a lower health status, with a higher lamb mortality (P sustainable. The less intensive system (1L/1Y) was both highly efficient from the animal standpoint and highly feed self-sufficient. The technical and economic results of this system were better than those of organic private farms in the same area and matched those of non-organic farms.

  12. Radio-transmitters do not affect seasonal productivity of female Golden-winged Warblers

    Science.gov (United States)

    Streby, Henry M.; Peterson, Sean M.; Gesmundo, Callie; Johnson, Michael K.; Fish, Alexander C.; Lehman, Justin A.; Andersen, David E.

    2013-01-01

    Investigating the potential effects of handling and marking techniques on study animals is important for correct interpretation of research results and to effect progress in data-collection methods. Few investigators have compared the reproductive output of radio-tagged and non-radio-tagged songbirds, and no one to date has examined the possible effect of radio-tagging adult songbirds on the survival of their fledglings. In 2011 and 2012, we compared several parameters of reproductive output of two groups of female Golden-winged Warblers (Vermivora chrysoptera) breeding in Minnesota, including 45 females with radio-transmitters and 73 females we did not capture, handle, or mark. We found no difference between groups in clutch sizes, hatching success, brood sizes, length of incubation and nestling stages, fledging success, number of fledglings, or survival of fledglings to independence. Thus, radio-tags had no measurable impact on the productivity of female Golden-winged Warblers. Our results build upon previous studies where investigators have reported no effects of radio-tagging on the breeding parameters of songbirds by also demonstrating no effect of radio-tagging through the post-fledging period and, therefore, the entire breeding season.

  13. ER type I signal peptidase subunit (LmSPC1) is essential for the survival of Locusta migratoria manilensis and affects moulting, feeding, reproduction and embryonic development.

    Science.gov (United States)

    Zhang, W; Xia, Y

    2014-06-01

    The endoplasmic reticulum type I signal peptidase complex (ER SPC) is a conserved enzyme that cleaves the signal peptides of secretory or membrane preproteins. The deletion of this enzyme leads to the accumulation of uncleaved proteins in biomembranes and cell death. However, the physiological functions of ER SPC in insects are not fully understood. Here, a catalytic subunit gene of ER SPC, LmSPC1, was cloned from Locusta migratoria manilensis and its physiological functions were analysed by RNA interference (RNAi). The LmSPC1 open reading frame encoded a protein of 178 amino acids with all five conserved regions of signal peptidases. RNAi-mediated knockdown of LmSPC1 resulted in high mortality. Sixty-nine per cent of dead nymphs died of abnormal moulting, corresponding to decreased activity of moulting fluid protease. Moreover, insects in the RNAi group experienced a decline in food intake, and a decrease in the secretion of total protein and digestive enzymes from midgut tissues to the midgut lumen. Furthermore, the females produced fewer eggs and eggs with disrupted embryogenesis. These results indicate that LmSPC1 is required for the secretion of secretory proteins, affects physiological functions, including moulting, feeding, reproduction and embryonic development, and is essential for survival. Therefore, LmSPC1 may be a potential target for locust control.

  14. Novel ETHE1 mutation in a carrier couple having prior offspring affected with ethylmalonic encephalopathy: Genetic analysis, clinical management and reproductive outcome.

    LENUS (Irish Health Repository)

    Walsh, David J

    2010-03-01

    Ethylmalonic encephalopathy (EE) is an autosomally recessive inherited disorder with a relentlessly progressive decline in neurological function, usually fatal by the age of ten. It is characterised by generalised hypotonia, psychomotor regression, spastic tetraparesis, dystonia, seizures and, eventually, global neurological failure. Approximately 50 reports have been published worldwide describing this devastating disease, most involving patients of Mediterranean or Arab origin. The fundamental defect in EE likely involves the impairment of a mitochondrial sulphur dioxygenase coded by the ETHE1 gene responsible for the catabolism of sulphide, which subsequently accumulates to toxic levels. A diagnosis of EE should initiate careful genetic evaluation and counselling, particularly if the parents intend to have additional offspring. The present report describes the diagnosis of EE in a reproductive endocrinology context, where both members of a non-consanguineous couple were confirmed to be carriers of an identical A↷G mutation. This previously unknown mutation at nucleotide position c.494 resulted in an amino acid substitution, p.Asp165Gly. Although consideration was given to in vitro fertilisation, embryo biopsy and single gene pre-implantation genetic diagnosis, the couple decided to first utilise a less aggressive therapeutic approach with donor sperm insemination. Pregnancy with a low risk of EE was indeed achieved; however, the infant was affected with a different anomaly (hypoplastic left heart). As this case demonstrates, prior to the initiation of fertility therapy, genetic analysis may be used to provide a confirmatory diagnosis when EE is suspected.

  15. Measurements of body composition during late gestation and lactation in first and second parity sows and its relationship to piglet production and post-weaning reproductive performance

    Science.gov (United States)

    The objective of this study was to characterize variation of multiple body condition traits in a white crossbred composite population of first- and second-parity sows and determine if these traits relate to litter production and reproductive parameters. As expected, parity 2 dams were heavier (P < 0...

  16. Swarm-Optimization-Based Affective Product Design Illustrated by a Mobile Phone Case-Study

    Directory of Open Access Journals (Sweden)

    Koffka Khan

    2012-05-01

    Full Text Available This paper presents a new approach of user-oriented design for transforming users’ perception into product elements design. An experimental study on mobile phones is conducted to examine how product form and product design parameters affect consumer’s perception of a product. The concept of Kansei Engineering is used to extract the experimental samples as a data base for neural networks (NNs with particle swarm optimization (PSO analysis. The result of numerical analysis suggests that mobile phone makers need to focus on particular design parameters to attract specific target user groups, in addition to product forms. This paper demonstrates the advantage of using KE-PSO for determining the optimal combination of product design parameters. Based on the analysis, we can use KE-PSO to suggest customers’ preferences for mobile phone design attributes that would be considered optimal by various user groups of all surveyed. They can be used for improvement and development of new future products.

  17. Reproductive function of the male obese Zucker rats: alteration in sperm production and sperm DNA damage.

    Science.gov (United States)

    Vendramini, V; Cedenho, A P; Miraglia, S M; Spaine, D M

    2014-02-01

    Obesity has been considered a public health issue in many countries and is of increasing concern for authorities over the past 6 years. The Zucker rat is a good experimental model for obesity and diabetes studies due to its metabolic characteristics that are similar to those developed by humans. A total of 12 obese Zucker rats and their lean littermates were killed in pubertal and young adult phases for assessing organ weights (testis and epididymis), testicular histomorphometric and stereological analyses, daily sperm production, and transit time in the epididymis. Sperm integrity was also investigated in the adult animals using the Comet assay. Alterations in organ weights, seminiferous epithelium architecture, sperm production, and transit time were noticed in the pubertal fatty rats. The volume density of the lymphatic space was decreased in both the ages. Adult animals had a significant increase in the extent of damage found in sperm DNA. Our results show for the first time that leptin receptor deficiency compromises sperm production during puberty and that genetic obese Zucker rats have increased sperm DNA fragmentation.

  18. Genes influencing milk production traits predominantly affect one of four biological pathways

    Directory of Open Access Journals (Sweden)

    Goddard Michael

    2008-01-01

    Full Text Available Abstract In this study we introduce a method that accounts for false positive and false negative results in attempting to estimate the true proportion of quantitative trait loci that affect two different traits. This method was applied to data from a genome scan that was used to detect QTL for three independent milk production traits, Australian Selection Index (ASI, protein percentage (P% and fat percentage corrected for protein percentage (F% – P%. These four different scenarios are attributed to four biological pathways: QTL that (1 increase or decrease total mammary gland production (affecting ASI only; (2 increase or decrease lactose synthesis resulting in the volume of milk being changed but without a change in protein or fat yield (affecting P% only; (3 increase or decrease protein synthesis while milk volume remains relatively constant (affecting ASI and P% in the same direction; (4 increase or decrease fat synthesis while the volume of milk remains relatively constant (affecting F% – P% only. The results indicate that of the positions that detected a gene, most affected one trait and not the others, though a small proportion (2.8% affected ASI and P% in the same direction.

  19. Factors Affecting Pheromone Production by the Pepper Weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae) and Collection Efficiency

    OpenAIRE

    Eller, Fred; Palmquist,Debra

    2014-01-01

    Several factors affecting pheromone production by male pepper weevils, Anthonomus eugenii Cano (Coleoptera: Curculionidae) as well as collection efficiency were investigated. Factors studied included: porous polymer adsorbents (Tenax versus Super Q), male age, time of day, male density, and male diet. Super Q was found to be a superior adsorbent for the male-produced alcohols and geranic acid as well as the plant-produced E-β-ocimene. Pheromone production increased with male age up to about a...

  20. Factors Affecting Pheromone Production by the Pepper Weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae) and Collection Efficiency

    OpenAIRE

    Eller, Fred J.; Debra E. Palmquist

    2014-01-01

    Several factors affecting pheromone production by male pepper weevils, Anthonomus eugenii Cano (Coleoptera: Curculionidae) as well as collection efficiency were investigated. Factors studied included: porous polymer adsorbents (Tenax versus Super Q), male age, time of day, male density, and male diet. Super Q was found to be a superior adsorbent for the male-produced alcohols and geranic acid as well as the plant-produced E-β-ocimene. Pheromone production increased with male age up to about ...

  1. Productive and Reproductive Performances of Female Etawah Crossbred Goats in Indonesia

    Directory of Open Access Journals (Sweden)

    I Ketut Sutama

    2009-03-01

    Full Text Available Although Etawah Crossbred (PE goat is considered to be dual purpose (meat and milk goat, it is mainly raised for meat production. Since early 1990, there has been a growing interest of the farmer in some places to raise PE goat for milk production without sacrificing its role to produce kids for meat. The average birth weight of PE kids varied widely (2.8 – 5 kg, resulted in a high variation in weaning weight (9 – 14 kg. A high pre-weaning mortality of 10 – 50% was a major source of lost in goat production in Indonesia, partly due to low birth weight and/or miss mothering ability. Young female PE goat reached puberty at 8 – 12 months of age and at body weight of about 18 – 22 kg or about 53 – 60% of mature body weight. Gestation length varied between 142 – 156 days, and first post partum estrous occurred at 3 – 5 months after parturition took place, resulted in 8 – 10 months of kidding intervals. Lactation period lasted for 5 – 8 months with total milk yield of 177 – 203 kg/lactation (average 0.85 kg/day. Although milk yield of PE goat was not as high as milk yield of some other dairy goats, the ability of PE goat to cope with harsh local environment, particularly climate and feed conditions, was an advantage. Therefore, raising PE goat would still be an important part of farmer activities in the rural areas in Indonesia.

  2. Inferring causal relationships between reproductive and metabolic health disorders and production traits in first-lactation US Holsteins using recursive models.

    Science.gov (United States)

    Dhakal, K; Tiezzi, F; Clay, J S; Maltecca, C

    2015-04-01

    Health disorders in dairy cows have a substantial effect on the profitability of a dairy enterprise because of loss in milk sales, culling of unhealthy cows, and replacement costs. Complex relationships exist between health disorders and production traits. Understanding the causal structures among these traits may help us disentangle these complex relationships. The principal objective of this study was to use producer-recorded data to explore phenotypic and genetic relationships among reproductive and metabolic health disorders and production traits in first-lactation US Holsteins. A total of 77,004 first-lactation daughters' records of 2,183 sires were analyzed using recursive models. Health data contained information on reproductive health disorders [retained placenta (RP); metritis (METR)] and metabolic health disorders [ketosis (KETO); displaced abomasum (DA)]. Production traits included mean milk yield (MY) from early lactation (mean MY from 6 to 60 d in milk and from 61 to 120 d in milk), peak milk yield (PMY), day in milk of peak milk yield (PeakD), and lactation persistency (LP). Three different sets of traits were analyzed in which recursive effects from each health disorder on culling, recursive effects of one health disorder on another health disorder and on MY, and recursive effects of each health disorder on production traits, including PeakD, PMY, and LP, were assumed. Different recursive Gaussian-threshold and threshold models were implemented in a Bayesian framework. Estimates of the structural coefficients obtained between health disorders and culling were positive; on the liability scale, the structural coefficients ranged from 0.929 to 1.590, confirming that the presence of a health disorder increased culling. Positive recursive effects of RP to METR (0.117) and of KETO to DA (0.122) were estimated, whereas recursive effects from health disorders to production traits were negligible in all cases. Heritability estimates of health disorders ranged

  3. Metaphor priming in sentence production: concrete pictures affect abstract language production.

    Science.gov (United States)

    Sato, Manami; Schafer, Amy J; Bergen, Benjamin K

    2015-03-01

    People speak metaphorically about abstract concepts-for instance, a person can be "full of love" or "have a lot of love to give." Over the past decade, research has begun to focus on how metaphors are processed during language comprehension. Much of this work suggests that understanding a metaphorical expression involves activating brain and body systems involved in perception and motor control. However, no research to date has asked whether the same is true while speakers produce language. We address this gap using a sentence production task. Its results demonstrate that visually activating a concrete source domain can trigger the use of metaphorical language drawn from that same concrete domain, even in sentences that are thematically unrelated to the primes, a metaphorical priming effect. This effect suggests that conceptual metaphors play a part in language production. It also shows that activation in the perceptual system that is not part of an intended message can nevertheless influence sentence formulation.

  4. ALOX5 gene variants affect eicosanoid production and response to fish oil supplementation

    Science.gov (United States)

    The objective of this study was to determine whether 5-lipoxygenase (ALOX5) gene variants associated with cardiovascular disease affect eicosanoid production by monocytes. The study was a randomized, double-masked, parallel intervention trial with fish oil (5.0 g of fish oil daily, containing 2.0 g ...

  5. Resource allocation to reproduction in animals.

    Science.gov (United States)

    Kooijman, Sebastiaan A L M; Lika, Konstadia

    2014-11-01

    The standard Dynamic Energy Budget (DEB) model assumes that a fraction κ of mobilised reserve is allocated to somatic maintenance plus growth, while the rest is allocated to maturity maintenance plus maturation (in embryos and juveniles) or reproduction (in adults). All DEB parameters have been estimated for 276 animal species from most large phyla and all chordate classes. The goodness of fit is generally excellent. We compared the estimated values of κ with those that would maximise reproduction in fully grown adults with abundant food. Only 13% of these species show a reproduction rate close to the maximum possible (assuming that κ can be controlled), another 4% have κ lower than the optimal value, and 83% have κ higher than the optimal value. Strong empirical support hence exists for the conclusion that reproduction is generally not maximised. We also compared the parameters of the wild chicken with those of races selected for meat and egg production and found that the latter indeed maximise reproduction in terms of κ, while surface-specific assimilation was not affected by selection. We suggest that small values of κ relate to the down-regulation of maximum body size, and large values to the down-regulation of reproduction. We briefly discuss the ecological context for these findings.

  6. Estimates of genetic parameters for visual scores and their correlation with production and reproductive traits in Brahman cattle

    Directory of Open Access Journals (Sweden)

    Tássia Souza Bertipaglia

    2012-06-01

    Full Text Available The objective of this study was to evaluate the association of visual scores of body structure, precocity and muscularity with production (body weight at 18 months and average daily gain and reproductive (scrotal circumference traits in Brahman cattle in order to determine the possible use of these scores as selection criteria to improve carcass quality. Covariance components were estimated by the restricted maximum likelihood method using an animal model that included contemporary group as fixed effect. A total of 1,116 observations of body structure, precocity and muscularity were used. Heritability was 0.39, 043 and 0.40 for body structure, precocity and muscularity, respectively. The genetic correlations were 0.79 between body structure and precocity, 0.87 between body structure and muscularity, and 0.91 between precocity and muscularity. The genetic correlations between visual scores and body weight at 18 months were positive (0.77, 0.57 and 0.59 for body structure, precocity and muscularity, respectively. Similar genetic correlations were observed between average daily gain and visual scores (0.60, 0.57 and 0.48, respectively, whereas the genetic correlations between scrotal circumference and these scores were low (0.13, 0.02, and 0.13. The results indicate that visual scores can be used as selection criteria in Brahman breeding programs. Favorable correlated responses should be seen in average daily gain and body weight at 18 months. However, no correlated response is expected for scrotal circumference.

  7. Incubation patterns in a central-place forager affect lifetime reproductive success: scaling of patterns from a foraging bout to a lifetime.

    Directory of Open Access Journals (Sweden)

    Akiko Shoji

    Full Text Available BACKGROUND: Long-lived seabirds face a conflict between current and lifelong reproductive success. During incubation shifts, egg neglect is sometimes necessary to avoid starvation, but may compromise the current reproductive attempt. However, factors underlying this decision process are poorly understood. We focus on the ancient murrelet, Synthliboramphus antiquus, an alcid with exceptionally long incubation shift lengths, and test the impact of environmental factors on incubation shift length in relation to reproductive success. METHODOLOGY/PRINCIPAL FINDINGS: Using an information theoretic approach, we show that incubation shift length was a strong predictor of reproductive success for ancient murrelets at Reef Island, Haida Gwaii, British Columbia, Canada during the 2007 and 2008 breeding seasons. The most important factors explaining an individual's shift length were egg size, wind speed and the length of the mate's previous shift. Wind speed and tide height were the two most important factors for determining foraging behavior, as measured by dive frequency and depth. CONCLUSIONS/SIGNIFICANCE: Our study demonstrates that (i species-specific reproductive strategies interact with environmental conditions such as wind speed to form multiple incubation patterns and (ii maintaining regular incubation shifts is an essential component of reproductive success.

  8. Antifungal activity of different natural dyes against traditional products affected fungal pathogens

    Institute of Scientific and Technical Information of China (English)

    R Mari selvam; AJA Ranjit Singh; K Kalirajan

    2012-01-01

    Objective: In the present study to evaluate the anti fungal activity of natural dyes against traditional products affected fungal pathogens. Methods: Many traditional craft products affected fungal pathogens were isolated using potato dextrose agar medium. The isolated fungus were identified by morphological and microscopically characterization using Alexopolus manual. 50μl of Turmeric, Terminalli, Guava and Henna natural dyes were poured into the wells of the culture plates. If antifungal activity was present on the plates, it was indicated by an inhibition zone surrounding the well containing the natural dye. Result: At a dose level of 50μl of terminalli dye was able to inhibit the growth of all the fungi tested. The absorbance rate of natural dyes analyzed by UV Spectrophotometer. The absorbance rate is high in terminalli (2.266) and turmeric (2.255). Conclusions: Natural dyes were bound with traditional products to give good colour and good antimicrobial activity against isolated fungal pathogens.

  9. Hormonal profiles, physiological parameters, and productive and reproductive performances of Girolando cows in the state of Ceará-Brazil

    Science.gov (United States)

    da Costa, Antônio Nélson Lima; Feitosa, José Valmir; Júnior, Péricles Afonso Montezuma; de Souza, Priscila Teixeira; de Araújo, Airton Alencar

    2015-02-01

    This study compared two breed groups of Girolando (½ Holstein ½ Gyr vs. ¾ Holstein ¼ Gyr) through analysis of physiological, productive, and reproductive parameters to determine the group best suited to rearing in a semiarid tropical climate. The experiment was conducted at the Companhia de Alimentos do Nordeste (CIALNE) farm, in the municipality of Umirim, State of Ceará, Brazil. Eighty cows were used in a 2 × 2 factorial study; 40 of each breed group were kept under an extensive system during the wet season and an intensive system during the dry season. The collection of physiological data and blood samples were obtained in the afternoon after milking. Rectal temperature (RT), surface temperature (ST), and respiratory rate (RR) were obtained for each cow after milking. Blood samples were obtained by tail vein puncture and were determined triiodothyronine (T3) and thyroxine (T4) and cortisol. The environmental parameters obtained were relative humidity (RH) and air temperature (AT), and from these, a temperature and humidity index (THI) was calculated. Pregnancy diagnosis (PD) was determined by ultrasonography 30 days after artificial insemination (AI). The milk production of each cow was recorded with automated milkings in the farm. The variables were expressed as mean and standard error, evaluated by ANOVA at 5 % probability using the Proc GLM of SAS. Chi-square test at 5 % probability was applied to data of pregnancy rate (PR) and the number of AI's to obtain pregnancy. It can be concluded that the breed group ½ Holstein ½ Gyr is most suited for farming under conditions of thermal stress.

  10. Rectal temperatures, respiratory rates, production, and reproduction performances of crossbred Girolando cows under heat stress in northeastern Brazil

    Science.gov (United States)

    da Costa, Antônio Nélson Lima; Feitosa, José Valmir; Montezuma, Péricles Afonso; de Souza, Priscila Teixeira; de Araújo, Airton Alencar

    2015-11-01

    This study compared the two breed groups of Girolando (½ Holstein ½ Gyr vs. ¾ Holstein ¼ Gyr) through analysis of the percentages (stressed or non-stressed cows) of rectal temperature (RT), respiratory rate (RR) and pregnancy rate (PR), and means of production and reproduction parameters to determine the group best suited to rearing in semiarid tropical climate. The experiment was conducted at the farm, in the municipality of Umirim, State of Ceará, Brazil. Two hundred and forty cows were used in a 2 × 2 factorial study; 120 of each group were kept under an intensive system during wet and dry seasons. The environmental parameters obtained were relative humidity (RH), air temperature (AT), and the temperature and humidity index (THI). Pregnancy diagnosis (PD) was determined by ultrasonography 30 days after artificial insemination (AI). The milk production of each cow was recorded with automated milkings in the farm. The variables were expressed as mean and standard error, evaluated by ANOVA at 5 % probability using the GLM procedure of SAS. Chi-square test at 5 % probability was applied to data of RT, RR, pregnancy rate (PR), and the number of AIs to obtain pregnancy. The majority of ½ Holstein cows showed mean values of RT and RR within the normal range in both periods and shifts. Most animals of the ¾ Holstein group exhibited the RR means above normal during the afternoon in the rainy and dry periods and RT means above normal during the afternoon in the dry period. After analyses, ½ Holstein crossbred cows are more capable of thermoregulating than ¾ Holstein cows under conditions of thermal stress, and the dry period was more impacting for bovine physiology with significant changes in physiological parameters, even for the first breed group. Knowledge of breed groups adapted to climatic conditions of northeastern Brazil can directly assist cattle farmers in selecting animals best adapted for forming herds.

  11. Short communication: A missense mutation in the PROP1 (prophet of Pit 1) gene affects male fertility and milk production traits in the US Holstein population.

    Science.gov (United States)

    Lan, X Y; Peñagaricano, F; DeJung, L; Weigel, K A; Khatib, H

    2013-02-01

    In previous studies, we reported significant associations of the POU1F1 pathway genes with reproduction and production traits in several dairy cattle populations. Polymorphisms in genes of this pathway were found to be associated with both female and male fertility traits in dairy cattle. The POU1F1 gene is a direct downstream target for the regulation of the prophet of Pit1 (PROP1) gene, also known as PROP paired-like homeobox 1. Interestingly, the position of PROP1 coincides with a quantitative trait locus affecting ovulation rate in cattle. Therefore, the objective of this study was to investigate whether PROP1 affects fertility and milk production traits in Holstein cattle. Using the DNA pooling sequencing approach, a missense single nucleotide polymorphism that replaces a histidine amino acid with an arginine was detected in exon 3 of PROP1. The arginine allele was found to be associated with a decrease in sire conception rate and an increase in productive life, protein yield, and net merit index in a population of 1,951 Holstein bulls. The transcription factors produced from the histidine and arginine isoforms are known to have different transcription, DNA binding, and regulation activities. As such, we propose that the association of the arginine isoform with decreased bull fertility is likely caused by reduced activity of this allele in male functions. The findings of this study suggest PROP1 polymorphisms as candidates in selection programs for fertility, health, and milk production traits in dairy cattle.

  12. Factors affecting Small-Scale Coffee Production in Githunguri District, Kenya

    Directory of Open Access Journals (Sweden)

    Margaret Njeri Gathura

    2013-09-01

    Full Text Available This study was carried out to determine the factors affecting small scale coffee production in Kenya. The establishments under study were small-scale coffee farms in Githunguri District.It was to determine whether marketing factors, finances, government policies and physical and human resources affect coffee production in Githunguri District. Primary sources included use of questionnaires, observation and interviews. Secondary sources included desk research, library research on journals, text books and factory publications. The target population was over 700,000 small-scale coffee producers in Kenya out of which the accessible population of 10,000 producers drawn from Githunguri District in Kiambu County was selected which a sample size of 120 respondents was sampled. Stratified sampling technique was employed to compare views among coffee producers from the various coffee societies in the area. Data analysis was both qualitative and quantitative using descriptive statistics. Data presentation was in form of tables to help interpret findings and generate conclusions that aided solutions to identified problems. The research established that marketing factors, finances, government policies and physical and human resources greatly affected coffee production. The study recommended that the government should encourage coffee production by formulating favorable marketing factors and other policies and provide finances to small scale coffee producers. Producers on the other hand should strive to provide conducive working environment to their workers so as to sustain them in their farms. This will help to improve coffee yields and quality.

  13. Deciphering the Costs of Reproduction in Mango – Vegetative Growth Matters

    Science.gov (United States)

    Capelli, Mathilde; Lauri, Pierre-Éric; Normand, Frédéric

    2016-01-01

    Irregular fruit production across successive years is a major issue that limits the profitability of most temperate and tropical fruit crops. It is particularly affected by the reciprocal relationships between vegetative and reproductive growth. The concept of the costs of reproduction is defined in terms of losses in the potential future reproductive success caused by current investment in reproduction. This concept, developed in ecology and evolutionary biology, could provide a methodological framework to analyze irregular bearing in fruit crops, especially in relation to the spatial scale at which studies are done. The objective of this study was to investigate the direct effects of reproduction during a growing cycle on reproduction during the following growing cycle and the indirect effects through vegetative growth between these two reproductive events, for four mango cultivars and during two growing cycles. Two spatial scales were considered: the growth unit (GU) and the scaffold branch. Costs of reproduction were detected between two successive reproductive events and between reproduction and vegetative growth. These costs were scale-dependent, generally detected at the GU scale and infrequently at the scaffold branch scale, suggesting partial branch autonomy with respect to processes underlying the effects of reproduction on vegetative growth. In contrast, the relationships between vegetative growth and reproduction were positive at the GU scale and at the scaffold branch scale in most cases, suggesting branch autonomy for the processes, mainly local, underlying flowering and fruiting. The negative effect of reproduction on vegetative growth prevailed over the positive effect of vegetative growth on the subsequent reproduction. The costs of reproduction were also cultivar-dependent. Those revealed at the GU scale were related to the bearing behavior of each cultivar. Our results put forward the crucial role of vegetative growth occurring between two

  14. Analyses of Sexual Reproductive Success in Transgenic and/or Mutant Plants

    Institute of Scientific and Technical Information of China (English)

    Cristiane P. G. Calixto; Gustavo H. Goldman; Maria Helena S. Goldman

    2009-01-01

    The pistil, the female reproductive organ of plants, is a key player in the success of sexual plant reproduction. Ultimately, the production of fruits and seeds depends on the proper pistil development and function. Therefore, the identification and characterization of pistil expressed genes is essential for a better understanding and manipulation of the plant reproduction process. For studying the function of pistil expressed genes, transgenic and/or mutant plants for the genes of interest are used. The present article provides a review of methods already exploited to analyze sexual reproductive success. We Intend to sup ply useful information and to guide future experiments in thestudy of genes affecting pistil development and function.

  15. Marijuana, the Endocannabinoid System and the Female Reproductive System

    Science.gov (United States)

    Brents, Lisa K.

    2016-01-01

    Marijuana use among women is highly prevalent, but the societal conversation on marijuana rarely focuses on how marijuana affects female reproduction and endocrinology. This article reviews the current scientific literature regarding marijuana use and hypothalamic-pituitary-ovarian (HPO) axis regulation, ovarian hormone production, the menstrual cycle, and fertility. Evidence suggests that marijuana can reduce female fertility by disrupting hypothalamic release of gonadotropin releasing hormone (GnRH), leading to reduced estrogen and progesterone production and anovulatory menstrual cycles. Tolerance to these effects has been shown in rhesus monkeys, but the effects of chronic marijuana use on human female reproduction are largely unknown. Marijuana-induced analgesia, drug reinforcement properties, tolerance, and dependence are influenced by ovarian hormones, with estrogen generally increasing and progesterone decreasing sensitivity to marijuana. Carefully controlled regulation of the Endocannabinoid System (ECS) is required for successful reproduction, and the exogenous cannabinoids in marijuana may disrupt the delicate balance of the ECS in the female reproductive system. PMID:27354844

  16. Pig slurry characteristics, nutrient balance and biogas production as affected by separation and acidification

    DEFF Research Database (Denmark)

    Sommer, S G; Hjorth, Maibritt; Leahy, J J

    2015-01-01

    Animal slurry is separated in order to avoid excessive nitrogen, phosphorus and potassium (NPK) fertilization of crops in the field. To enhance fertilizer efficiency further, slurry and its separation products may be acidified, for instance in animal houses. The current study quantified the effects...... of these treatments, both individually and in combination, on fertilizer efficiency, energy production and heavy metal accumulation as a result of manure management. Acidification increased the availability of N to plants in the manure applied, and provided a better match between plant-available NPK in the manure...... and separation fraction applied to fields and crop need. Total biogas production was not affected by separation, whereas acidification reduced biogas production because the process was inhibited by a low pH and a high sulphur concentration. The amount of copper applied per hectare in the liquid manure...

  17. Quality and safety aspects of meat products as affected by various physical manipulations of packaging materials.

    Science.gov (United States)

    Lee, Keun Taik

    2010-09-01

    This article explores the effects of physically manipulated packaging materials on the quality and safety of meat products. Recently, innovative measures for improving quality and extending the shelf-life of packaged meat products have been developed, utilizing technologies including barrier film, active packaging, nanotechnology, microperforation, irradiation, plasma and far-infrared ray (FIR) treatments. Despite these developments, each technology has peculiar drawbacks which will need to be addressed by meat scientists in the future. To develop successful meat packaging systems, key product characteristics affecting stability, environmental conditions during storage until consumption, and consumers' packaging expectations must all be taken into consideration. Furthermore, the safety issues related to packaging materials must also be taken into account when processing, packaging and storing meat products.

  18. Population structure and fruit production of Pyrus bourgaeana D. are affected by land-use

    Science.gov (United States)

    Arenas-Castro, Salvador; Fernández-Haeger, Juan; Jordano-Barbudo, Diego

    2016-11-01

    The Iberian wild pear (Pyrus bourgaeana D.) is a rare, fleshy-fruited tree restricted to dehesas and evergreen sclerophyllous Mediterranean forests in the southwestern Iberian Peninsula. It produces palatable fruits and leaves attractive to different species groups, playing an important trophic role in the ecological networks of Mediterranean ecosystems. However, the intensification in the traditional land-use linked to these areas could threaten the stability of the wild pear populations in the short/medium-term. In order to determine the population dynamics of this relevant species in relation to the land-use history, we selected two populations (southern Spain) subjected to different land-use management, dehesa (D) and abandoned olive grove (AOG). An analysis of 122 adult trees reported an overall density of 0.6 trees ha-1. The tree age was estimated by tree-rings analysis in all adult trees. Dendrometric parameters, reproductive features, and germination rates were also measured. Regeneration was clearly biased, as evidenced by the truncated age structure. A low correlation (R2 = 34%) between age and DBH (diameter at breast height) (244 cores analysed) showed that diameter seems not to be a reliable predictor of tree age. Trees from AOG populations had significantly-higher values of DBH, height and crown diameter, but were less productive in terms of fruits and seeds. Nested analysis of variance showed significant variation in fruit production, fruit size, dry mass, water content and seed viability. There were also significant differences in masting. No evidence was found to demonstrate that fruit production, seed viability, or germination rate influence the low natural recruitment of this species. These findings indicate that the traditional agrosilvopastoral practices carried out in the study area for decades, and its subsequent intensification, have strongly influenced the ecological structure of the Iberian wild pear populations at the local scale, which

  19. Influence of Exogenous Reproductive Hormones on Specific Antibody Production in Genital Secretions after Vaginal Vaccination with Recombinant Cholera Toxin B Subunit in Humans

    OpenAIRE

    Wassen, Lotta; Jertborn, Marianne

    2006-01-01

    The objective of this study was to investigate the influence of exogenous reproductive hormones on the local and systemic production of specific immunoglobulin A (IgA) and IgG antibodies after vaginal vaccination with recombinant cholera toxin subunit B (CTB). Three groups of women using either progesterone-containing intrauterine devices (n = 9), oral contraceptives (n = 8), or no hormonal contraceptive methods (n = 9) were vaginally immunized twice, 2 weeks apart. Cervical secretions, vagin...

  20. Long-Term Moderate Oxidative Stress Decreased Ovarian Reproductive Function by Reducing Follicle Quality and Progesterone Production

    Science.gov (United States)

    Lai, Zhiwen; Tian, Yong; Fang, Li; Wu, Meng; Xiong, Jiaqiang; Qin, Xian; Luo, Aiyue; Wang, Shixuan

    2016-01-01

    decreased female reproductive function caused by long-term moderate oxidative damage may be due to a decrease in follicle quality and progesterone production. PMID:27676390

  1. Genotypic and phenotypic diversity does not affect productivity and drought response in competitive stands of Trifolium repens

    Directory of Open Access Journals (Sweden)

    Heidrun eHuber

    2016-03-01

    Full Text Available Clonal plants can form dense canopies in which plants of different genetic origin are competing for the uptake of essential resources. The competitive relationships among these clones are likely to be affected by extreme environmental conditions, such as prolonged drought spells, which are predicted to occur more frequently due to global climate change. This, in turn, may alter characteristics of the ecological system and its associated functioning.We hypothesized that the relative success of individual clones will depend on the size of the ramets as ramets with larger leaves and longer petioles (large ramets were predicted to have a competitive advantage in terms of increased light interception over smaller-sized ramets. Under drier conditions the relative performances of genotypes were expected to change leading to a change in genotype ranking. We also hypothesized that increased genotypic and phenotypic diversity will increase stand performance and resistance to drought. These hypotheses and the mechanisms responsible for shifts in competitive relationships were investigated by subjecting genotypes of the important pasture legume Trifolium repens to competition with either genetically identical clones, genetically different but similarly sized clones, or genetically as well as morphologically different clones under well-watered and dry conditions.Competitive relationships were affected by ramet size with large genotypes outperforming small genotypes in diverse stands in terms of biomass production. However, large genotypes also produced relatively fewer ramets than small genotypes and could not benefit in terms of clonal reproduction from competing with smaller genotypes, indicating that evolutionary shifts in genotype composition will depend on whether ramet size or ramet number is under selection. In contrast to our hypotheses, diversity did not increase stand performance under different selection regimes and genotype ranking was hardly

  2. Impact of Marine Drugs on Animal Reproductive Processes

    Directory of Open Access Journals (Sweden)

    Elisabetta Tosti

    2009-11-01

    Full Text Available The discovery and description of bioactive substances from natural sources has been a research topic for the last 50 years. In this respect, marine animals have been used to extract many new compounds exerting different actions. Reproduction is a complex process whose main steps are the production and maturation of gametes, their activation, the fertilisation and the beginning of development. In the literature it has been shown that many substances extracted from marine organisms may have profound influence on the reproductive behaviour, function and reproductive strategies and survival of species. However, despite the central importance of reproduction and thus the maintenance of species, there are still few studies on how reproductive mechanisms are impacted by marine bioactive drugs. At present, studies in either marine and terrestrial animals have been particularly important in identifying what specific fine reproductive mechanisms are affected by marine-derived substances. In this review we describe the main steps of the biology of reproduction and the impact of substances from marine environment and organisms on the reproductive processes.

  3. Effects of calcium salts of polyunsaturated fatty acids on productive and reproductive parameters of lactating Holstein cows.

    Science.gov (United States)

    Reis, M M; Cooke, R F; Ranches, J; Vasconcelos, J L M

    2012-12-01

    Two experiments evaluated milk production, serum progesterone and insulin, and reproductive performance of lactating Holstein cows receiving or not receiving Ca salts of polyunsaturated fatty acids (PUFA), or receiving Ca salts of PUFA at different daily frequencies. In experiment 1, 1,125 cows randomly distributed in 10 freestall barns were enrolled. Barns were assigned randomly to receive a high-concentrate diet containing (PF) or not containing (control, CON) 1.1% (dry matter basis) Ca salts of PUFA. Diets were offered 6 times daily, whereas the Ca salts of PUFA were included in the PF treatment in the first feeding of the day. In experiment 2, 1,572 cows were randomly distributed in 10 freestall barns, which were assigned randomly to receive a diet similar to PF, but with Ca salts of PUFA included only in the first feeding of the day (PF1X), or equally distributed across all 6 feedings (PF6X). During both experiments, cows were artificially inseminated 12 h after the onset of estrus. Once per month, cows that did not conceive to artificial insemination were assigned to a fixed-time embryo transfer protocol. Pregnancy was determined via transrectal ultrasonography 28 and 60 d after expected ovulation. Pregnancy loss was considered in cows that were pregnant on d 28 but nonpregnant on d 60. During both experiments, feed intake, milk yield, and milk protein and fat content were recorded weekly. Blood samples were collected concurrently with embryo transfer. During experiment 1, feed intake was similar between treatments. Compared with CON, PF cows had greater milk yield (37.8 vs. 35.3 kg/d), and reduced milk fat content (3.41 vs. 3.55%). However, PF cows had reduced pregnancy losses per service compared with CON (12.6 vs. 18.3%). Serum progesterone was greater and serum insulin tended to be greater in primiparous cows receiving PF compared with CON cohorts (4.50 vs. 3.67 ng of progesterone/mL, and 10.4 vs. 7.5 µUI of insulin/mL). During experiment 2, no treatment

  4. Fermentation conditions that affect clavulanic acid production in Streptomyces clavuligerus: a systematic review

    Directory of Open Access Journals (Sweden)

    Hooi-Leng eSer

    2016-04-01

    Full Text Available The β-lactamase inhibitor, clavulanic acid is frequently used in combination with β-lactam antibiotics to treat a wide spectrum of infectious diseases. Clavulanic acid prevents drug resistance by pathogens against these β-lactam antibiotics by preventing the degradation of the β-lactam ring, thus ensuring eradication of these harmful microorganisms from the host. This systematic review provides an overview on the fermentation conditions that affect the production of clavulanic acid in the firstly described producer, Streptomyces clavuligerus. A thorough search was conducted using predefined terms in several electronic databases (PubMed, Medline, ScienceDirect, EBSCO, from database inception to June 30th 2015. Studies must involve wild-type Streptomyces clavuligerus, and full texts needed to be available. A total of 29 eligible articles were identified. Based on the literature, several factors were identified that could affect the production of clavulanic acid in S. clavuligerus. The addition of glycerol or other vegetable oils (e.g. olive oil, corn oil could potentially affect clavulanic acid production. Furthermore, some amino acids such as arginine and ornithine, could serve as potential precursors to increase clavulanic acid yield. The comparison of different fermentation systems revealed that fed-batch fermentation yields higher amounts of clavulanic acid as compared to batch fermentation, probably due to the maintenance of substrates and constant monitoring of certain entities (such as pH, oxygen availability, etc.. Overall, these findings provide vital knowledge and insight that could assist media optimization and fermentation design for clavulanic acid production in S. clavuligerus.

  5. Fermentation Conditions that Affect Clavulanic Acid Production in Streptomyces clavuligerus: A Systematic Review.

    Science.gov (United States)

    Ser, Hooi-Leng; Law, Jodi Woan-Fei; Chaiyakunapruk, Nathorn; Jacob, Sabrina Anne; Palanisamy, Uma Devi; Chan, Kok-Gan; Goh, Bey-Hing; Lee, Learn-Han

    2016-01-01

    The β-lactamase inhibitor, clavulanic acid is frequently used in combination with β-lactam antibiotics to treat a wide spectrum of infectious diseases. Clavulanic acid prevents drug resistance by pathogens against these β-lactam antibiotics by preventing the degradation of the β-lactam ring, thus ensuring eradication of these harmful microorganisms from the host. This systematic review provides an overview on the fermentation conditions that affect the production of clavulanic acid in the firstly described producer, Streptomyces clavuligerus. A thorough search was conducted using predefined terms in several electronic databases (PubMed, Medline, ScienceDirect, EBSCO), from database inception to June 30th 2015. Studies must involve wild-type Streptomyces clavuligerus, and full texts needed to be available. A total of 29 eligible articles were identified. Based on the literature, several factors were identified that could affect the production of clavulanic acid in S. clavuligerus. The addition of glycerol or other vegetable oils (e.g., olive oil, corn oil) could potentially affect clavulanic acid production. Furthermore, some amino acids such as arginine and ornithine, could serve as potential precursors to increase clavulanic acid yield. The comparison of different fermentation systems revealed that fed-batch fermentation yields higher amounts of clavulanic acid as compared to batch fermentation, probably due to the maintenance of substrates and constant monitoring of certain entities (such as pH, oxygen availability, etc.). Overall, these findings provide vital knowledge and insight that could assist media optimization and fermentation design for clavulanic acid production in S. clavuligerus.

  6. Do non-native plant species affect the shape of productivity-diversity relationships?

    Science.gov (United States)

    Drake, J.M.; Cleland, E.E.; Horner-Devine, M. C.; Fleishman, E.; Bowles, C.; Smith, M.D.; Carney, K.; Emery, S.; Gramling, J.; Vandermast, D.B.; Grace, J.B.

    2008-01-01

    The relationship between ecosystem processes and species richness is an active area of research and speculation. Both theoretical and experimental studies have been conducted in numerous ecosystems. One finding of these studies is that the shape of the relationship between productivity and species richness varies considerably among ecosystems and at different spatial scales, though little is known about the relative importance of physical and biological mechanisms causing this variation. Moreover, despite widespread concern about changes in species' global distributions, it remains unclear if and how such large-scale changes may affect this relationship. We present a new conceptual model of how invasive species might modulate relationships between primary production and species richness. We tested this model using long-term data on relationships between aboveground net primary production and species richness in six North American terrestrial ecosystems. We show that primary production and abundance of non-native species are both significant predictors of species richness, though we fail to detect effects of invasion extent on the shapes of the relationship between species richness and primary production.

  7. Phenolic compounds affect production of pyocyanin, swarming motility and biofilm formation of Pseudomonas aeruginosa

    Institute of Scientific and Technical Information of China (English)

    Aylin Ugurlu; Aysegul Karahasan Yagci; Seyhan Ulusoy; Burak Aksu; Gulgun Bosgelmez-Tinaz

    2016-01-01

    Objective: To investigate the effects of plant-derived phenolic compounds (i.e. caffeic acid, cinnamic acid, ferulic acid and vanillic acid) on the production of quorum sensing regulated virulence factors such as pyocyanin, biofilm formation and swarming motility of Pseudomonas aeruginosa (P. aeruginosa) isolates. Methods: Fourteen clinical P. aeruginosa isolates obtained from urine samples and P. aeruginosa PA01 strain were included in the study. The antibacterial effects of phenolic compounds were screened by well diffusion assay. Pyocyanin and biofilm ac-tivity were measured from culture supernatants and the absorbance values were measured using a spectrophotometer. Swarming plates supplemented with phenolic acids were point inoculated with P. aeruginosa strains and the ability to swarm was determined by measuring the distance of swarming from the central inoculation site. Results: Tested phenolic compounds reduced the production of pyocyanin and biofilm formation without affecting growth compared to untreated cultures. Moreover, these compounds blocked about 50% of biofilm production and swarming motility in P. aeruginosa isolates. Conclusions: We may suggest that if swarming and consecutive biofilm formation could be inhibited by the natural products as shown in our study, the bacteria could not attach to the surfaces and produce chronic infections. Antimicrobials and natural products could be combined and the dosage of antimicrobials could be reduced to overcome antimicrobial resistance and drug side effects.

  8. Phenolic compounds affect production of pyocyanin, swarming motility and biofilm formation of Pseudomonas aeruginosa

    Institute of Scientific and Technical Information of China (English)

    Aylin Ugurlu; Aysegul Karahasan Yagci; Seyhan Ulusoy; Burak Aksu; Gulgun Bosgelmez-Tinaz

    2016-01-01

    Objective: To investigate the effects of plant-derived phenolic compounds(i.e. caffeic acid, cinnamic acid, ferulic acid and vanillic acid) on the production of quorum sensing regulated virulence factors such as pyocyanin, biofilm formation and swarming motility of Pseudomonas aeruginosa(P. aeruginosa) isolates.Methods: Fourteen clinical P. aeruginosa isolates obtained from urine samples and P. aeruginosa PA01 strain were included in the study. The antibacterial effects of phenolic compounds were screened by well diffusion assay. Pyocyanin and biofilm activity were measured from culture supernatants and the absorbance values were measured using a spectrophotometer. Swarming plates supplemented with phenolic acids were point inoculated with P. aeruginosa strains and the ability to swarm was determined by measuring the distance of swarming from the central inoculation site.Results: Tested phenolic compounds reduced the production of pyocyanin and biofilm formation without affecting growth compared to untreated cultures. Moreover, these compounds blocked about 50% of biofilm production and swarming motility in P. aeruginosa isolates.Conclusions: We may suggest that if swarming and consecutive biofilm formation could be inhibited by the natural products as shown in our study, the bacteria could not attach to the surfaces and produce chronic infections. Antimicrobials and natural products could be combined and the dosage of antimicrobials could be reduced to overcome antimicrobial resistance and drug side effects.

  9. Nutrition and reproduction in women.

    Science.gov (United States)

    2006-01-01

    Malnutrition is a major problem in developing countries, and obesity and eating disorders are increasingly common in developing as well as developed countries. The reproductive axis is closely linked to nutritional status, especially undernutrition in the female, and inhibitory pathways involving detectors in the hind brain suppress ovulation in subjects with weight loss. Recovery may occur after minimal reacquisition of weight because energy balance is more important than body fat mass. Anorexia nervosa and bulimia nervosa affect up to 5% of women of reproductive age causing amenorrhoea, infertility and, in those who do conceive, an increased likelihood of miscarriage. Obesity can affect reproduction through fat cell metabolism, steroids and secretion of proteins such as leptin and adiponectin and through changes induced at the level of important homeostatic factors such as pancreatic secretion of insulin, androgen synthesis by the ovary and sex hormone-binding globulin (SHBG) production by the liver. WHO estimates that 9 to 25% of women in developed countries are severely obese, and obese mothers are much more likely to have obese children, especially if they have gestational diabetes. Obesity-associated anovulation may lead to infertility and to a higher risk of miscarriage. Management of anovulation with obesity involves diet and exercise as well as standard approaches to ovulation induction. Many obese women conceive without assistance, but pregnancies in obese women have increased rates of pregnancy-associated hypertension, gestational diabetes, large babies, Cesarean section and perinatal mortality and morbidity. Among contraceptors, the fear of weight gain affects uptake and continuation of hormonal contraceptives, although existing trials indicate that any such effects are small. For all methods of hormonal contraception, weight above 70 kg is associated with increased failure rates.

  10. Pregnancy Followed by Delivery May Affect Circulating Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Levels in Women of Reproductive Age

    Directory of Open Access Journals (Sweden)

    Mehmet Balin

    2012-01-01

    Full Text Available Background/Objective. It is known that menopause or lack of endogenous estrogen is a risk factor for endothelial dysfunction and CAD. Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1 is involved inmultiple phases of vascular dysfunction.The purpose of the current study was to determine the association between soluble LOX-1 (sLOX-1 and pregnancy followed by delivery in women of reproductive age. Materials/Methods. Sixty-eight subjects with pregnancy followed by delivery (group 1 and 57 subjects with nongravidity (group 2 were included in this study. Levels of sLOX-1 were measured in serum by EL SA. Results. Plasma levels of sLOX-1 were significantly lower in Group 1 than Group 2 in women of reproductive age (0.52±0.18 ng/mL and 0.78±0.13, resp., <0.001. There were strong correlations between sLOX-1 levels and the number of gravida (=−0.645, <0.001. The levels of sLOX-1 highly correlated with the number of parous (=−0.683, <0.001. Conclusion. Our study demonstrated that serum sLOX-1 levels were associated with pregnancy followed by delivery that might predict endothelial dysfunction. We conclude that pregnancy followed by delivery may delay the beginning and progress of arteriosclerosis and its clinical manifestations in women of reproductive age.

  11. Do abundance and proximity of the alien Impatiens glandulifera affect pollination and reproductive success of two sympatric co-flowering native species?

    Directory of Open Access Journals (Sweden)

    Anne-Laure Jacquemart

    2012-12-01

    Full Text Available In invasion ecology, potential impacts of aliens on native flora are still under debate. Our aim was to determine the pollinator mediated effects of both proximity and abundance of an alien species on the reproductive success of natives. We chose the highly invasive Impatiens glandulifera and two native species: Epilobium angustifolium and Aconitum napellus ssp. lusitanicum. These species share characteristics allowing for pollination interactions: similar biotopes, overlapping flowering periods and same main pollinators. The effects of abundance (5, 25 and 100 individuals and proximity (0 and 15 m of the alien on visitation rate, insect behaviour, pollen deposition and reproductive success of both natives were investigated during 2 flowering seasons. We used centred visitation rates as they can be directly interpreted as a positive or negative effect of the invasive.Both abundance and proximity of the alien increased bumblebee visitation rates to both natives. On the other hand, abundance of the exotic species had a slight negative effect on honeybee visits to natives while its proximity had no effect. The behaviour of bumblebees changed as visitors left significantly more often the native plants for I. glandulifera when its abundance increased. As a consequence of this “inconstancy”, bees deposited considerable quantities of alien pollen on native stigmas. Nevertheless, this interspecific pollen transfer did not decrease seed set in natives. Self-compatibility and high attractiveness of both native species probably alleviate the risk of altered pollinator services and reproductive success due to the invader in natural populations.

  12. TLR-mediated NF-kB-dependent cytokine production is differently affected by HIV therapeutics

    DEFF Research Database (Denmark)

    Melchjorsen, Jesper; Paludan, Søren Riis; Mogensen, Trine

      Pathogen-recognizing Toll-like receptors 2 (TLR2) and TLR4 are known to recognize a number of pathogens, including E.Coli, S. Pneumonia and N. Meningococcus. We have studied whether a number of HIV therapeutics affect immediate proinflammatory cytokine responses in cell cultures. Preliminary...... results suggest an opposing effect of the drugs Tenofovir and Retrovir (AZT) on TLR-mediated production of NF-kappaB-dependent proinflammatory cytokines. We present data on the mechanisms behind the drug-mediated remodeling of innate immune activation and how the drugs effect early host...

  13. Does Mixed Reimbursement Schemes Affect Hospital Activity and Productivity? An Analysis of the Case of Denmark

    DEFF Research Database (Denmark)

    Hansen, Xenia Brun; Bech, Mickael; Jakobsen, Mads Leth;

    2013-01-01

    The majority of public hospitals in Scandinavia are reimbursed through a mixture of two prospective reimbursement schemes, block grants (a fixed amount independent of the number of patients treated) and activity-based financing (ABF). This article contributes theoretically to the existing...... whether different incentives affects the performance of hospitals regarding activity and productivity differently. Information on Danish reimbursement schemes has been collected from documents provided by the regional governments and through interviews with regional administrations. The data cover...... and region we show that there have not been any significant changes in the number of hospital discharges for any of the regions from 2007 to 2010 within any of the treatment groups....

  14. Genetic aspects of female reproduction

    NARCIS (Netherlands)

    Collins, J.; Diedrich, K.; Franks, S.; Geraedts, J. P. M.; Jacobs, P. A.; Karges, B.; Kennedy, S.; Marozzi, A.; Regan, L.; Baird, D. T.; Crosignani, P. G.; Devroey, P.; Diczfalusy, E.; Evers, J. L. H.; Fauser, B. C. J. M.; Fraser, L.; Gianaroli, L.; Glasier, A.; Liebaers, I.; Ragni, G.; Sunde, A.; Tarlatzis, B.; Van Steirteghem, A.

    2008-01-01

    BACKGROUND: Sexual reproduction provides the means for preserving genetic identity and in turn, genetic variability may affect the ability to reproduce. This review aims to summarize current research on genetic diagnosis and genetic causes of reproductive disorders.METHODS: Searches were done by sub

  15. Isolation and characterization of Rhizobium meliloti mutants affected in exopolysaccharide production.

    Science.gov (United States)

    Rodríguez-Navarro, D N; Palomares, A J; Casadesús, J

    1991-06-01

    Rhizobium meliloti mutants affected in the production of exopolysaccharide (EPS) were isolated after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. The mutants were classified into three phenotypic classes: (I) Exo-, rough mutants lacking exopolysaccharide; (II) Exos (for "small") which form tiny, compact colonies and synthesize reduced amounts of EPS; and (III) Exoc (for "constitutive"), hypermucoid mutants which overproduce EPS. Hypermucoid strains showed increased resistance to desiccation. All the mutants were able to nodulate, although a significant decrease in infectivity degree and/or competitiveness was found in rough and compact strains. Two mutants proved to be deficient in nitrogen fixation. Complementation analysis with cloned R. meliloti exo genes could not be applied to the study of these Fix- mutants because introduction of plasmids derived from cosmid vector pLAFR1 caused loss of nodulating ability. However, complementation of calcofluor staining and EPS production was observed. Complementation with certain exo genes also caused a marked increase in motility.

  16. An Affective Prototyping Approach for the User-Satisfying Assistive Products

    DEFF Research Database (Denmark)

    Lai, Yoke Chin

    2013-01-01

    This paper outlines the key concepts of a study whose main aim is to devise an integrated design method for efficiently designing usable assistive products. The study grounds on Affective Design principle formulated for user’s needs, emotions and experience handling in an attempt to outline...... an integrated approach so that the key concept of participatory design could be practiced. Incorporating the maturing Augmented Reality technology is expected to create a design setting that could motivate active end-user involvement from the start throughout the product design process. With the aids...... of ontological modeling approach, the design setting is envisioned suitable for disabled and elderly end users participation and to support capturing the abstract but valuable user’s real needs that are influenced by emotions and experiences. The ontology model will outline the basis of a design knowledge...

  17. Factors affecting release of ethanol vapour in active modified atmosphere packaging systems for horticultural products

    Directory of Open Access Journals (Sweden)

    Weerawate Utto

    2014-04-01

    Full Text Available The active modified atmosphere packaging (active MAP system , which provides interactive postharvest control , using ethanol vapour controlled release, is one of the current interests in the development of active packaging for horticultural products. A number of published research work have discussed the relationship between the effectiveness of ethanol vapour and its concentration in the package headspace, including its effect on postharvest decay and physiological controls. This is of importance because a controlled release system should release and maintain ethanol vapour at effective concentrations during the desired storage period. A balance among the mass transfer processes of ethanol vapour in the package results in ethanol vapour accumulation in the package headspace. Key factors affecting these processes include ethanol loading, packaging material, packaged product and storage environment (temperature and relative h umidity. This article reviews their influences and discusses future work required to better understand their influences on ethanol vapour release and accumulations in active MAP.

  18. Effect of Vitamin E-Selenium Administration during Late Gestation on Productive and Reproductive Performance in Dairy Buffaloes and on Growth Performance of their Calves

    Directory of Open Access Journals (Sweden)

    Z. I. Qureshi, M. Siddiq, L. A. Lodhi, G. Muhammad1 and H. Jamil

    2010-04-01

    Full Text Available The present study was planned to evaluate the efficacy of vitamin E-selenium administration during last month of gestation in improving the productive and reproductive performance of dairy buffaloes and on growth performance of their calves. For this purpose, 40 Nili-Ravi buffaloes in their last month of pregnancy were randomly divided into two groups. The buffaloes in group 1 were treated with 10 ml of vitamin E-Se (Farvet Holland, containing 50 mg of vitamin E and 0.5 mg of sodium selenite/ml intramuscularly on day 30 and 15 prior to expected date of parturition, while buffaloes in group II served as control. All the experimental buffaloes were kept under similar management and feeding conditions. The data on productive performance showed that the colostrum production was 22% higher (P0.05 in Vit E-Se treated buffaloes (706.90  314.29L as compared to control group (663.85  225.46L. Data on reproductive performance showed that vitamin E-Se administration significantly (P0.05. It was inferred that the administration of vitamin E-Se during late gestation in buffaloes have beneficial effect on colostrum production, post partum estrus rates and conception rates.

  19. Flower diversity and bee reproduction in an arid ecosystem

    Directory of Open Access Journals (Sweden)

    Jimena Dorado

    2016-07-01

    Full Text Available Background: Diverse flower communities are more stable in floral resource production along the flowering season, but the question about how the diversity and stability of resources affect pollinator reproduction remains open. High plant diversity could favor short foraging trips, which in turn would enhance bee fitness. In addition to plant diversity, greater temporal stability of floral resources in diverse communities could favor pollinator fitness because such communities are likely to occupy the phenological space more broadly, increasing floral availability for pollinators throughout the season. In addition, this potential effect of flower diversity on bee reproduction could be stronger for generalist pollinators because they can use a broader floral spectrum. Based on above arguments we predicted that pollinator reproduction would be positively correlated to flower diversity, and to temporal stability in flower production, and that this relationship would be stronger for the most generalized pollinator species. Materials and Methods: Using structural equation models, we evaluated the effect of these variables and other ecological factors on three estimates of bee reproduction (average number of brood cells per nest per site, total number of brood cells per site, and total number of nests per site, and whether such effects were modulated by bee generalization on floral resources. Results: Contrary to our expectations, flower diversity had no effect on bee reproduction, stability in flower production had a weakly negative effect on one of the bee reproductive variables, and the strength of the fitness-diversity relationship was unrelated to bee generalization. In contrast, elevation had a negative effect on bee reproduction, despite the narrow elevation range encompassed by our sites. Discussion: Flower diversity did not affect the reproduction of the solitary bees studied here. This result could stem from the context dependence of the

  20. How do strategic decisions and operative practices affect operating room productivity?

    Science.gov (United States)

    Peltokorpi, Antti

    2011-12-01

    Surgical operating rooms are cost-intensive parts of health service production. Managing operating units efficiently is essential when hospitals and healthcare systems aim to maximize health outcomes with limited resources. Previous research about operating room management has focused on studying the effect of management practices and decisions on efficiency by utilizing mainly modeling approach or before-after analysis in single hospital case. The purpose of this research is to analyze the synergic effect of strategic decisions and operative management practices on operating room productivity and to use a multiple case study method enabling statistical hypothesis testing with empirical data. 11 hypotheses that propose connections between the use of strategic and operative practices and productivity were tested in a multi-hospital study that included 26 units. The results indicate that operative practices, such as personnel management, case scheduling and performance measurement, affect productivity more remarkably than do strategic decisions that relate to, e.g., units' size, scope or academic status. Units with different strategic positions should apply different operative practices: Focused hospital units benefit most from sophisticated case scheduling and parallel processing whereas central and ambulatory units should apply flexible working hours, incentives and multi-skilled personnel. Operating units should be more active in applying management practices which are adequate for their strategic orientation.

  1. Factors Affecting Pheromone Production by the Pepper Weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae) and Collection Efficiency.

    Science.gov (United States)

    Eller, Fred J; Palmquist, Debra E

    2014-11-18

    Several factors affecting pheromone production by male pepper weevils, Anthonomus eugenii Cano (Coleoptera: Curculionidae) as well as collection efficiency were investigated. Factors studied included: porous polymer adsorbents (Tenax versus Super Q), male age, time of day, male density, and male diet. Super Q was found to be a superior adsorbent for the male-produced alcohols and geranic acid as well as the plant-produced E-β-ocimene. Pheromone production increased with male age up to about age 15 days old and then tapered off. Male pepper weevils produced the highest amount of pheromone between noon and 2 pm (i.e., 4 to 6 h after "lights on") and were producing ca. 800 ng/h during this period. Thereafter, pheromone production decreased and was extremely low during the scotophase (i.e., ca. 12 ng/h). Male pepper weevil density had a significant effect on both release rate and pheromone composition. Pheromone production on a per male basis was highest for individual males and the percentage of geranic acid in the blend was lowest for individual males. Male pepper weevils produced only extremely low amounts of pheromone when feeding on artificial diet; however, they produced very high amounts when on fresh peppers. Together, this information will be useful in designing better attractant lures for pepper weevils.

  2. Factors Affecting Pheromone Production by the Pepper Weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae and Collection Efficiency

    Directory of Open Access Journals (Sweden)

    Fred J. Eller

    2014-11-01

    Full Text Available Several factors affecting pheromone production by male pepper weevils, Anthonomus eugenii Cano (Coleoptera: Curculionidae as well as collection efficiency were investigated. Factors studied included: porous polymer adsorbents (Tenax versus Super Q, male age, time of day, male density, and male diet. Super Q was found to be a superior adsorbent for the male-produced alcohols and geranic acid as well as the plant-produced E-β-ocimene. Pheromone production increased with male age up to about age 15 days old and then tapered off. Male pepper weevils produced the highest amount of pheromone between noon and 2 pm (i.e., 4 to 6 h after “lights on” and were producing ca. 800 ng/h during this period. Thereafter, pheromone production decreased and was extremely low during the scotophase (i.e., ca. 12 ng/h. Male pepper weevil density had a significant effect on both release rate and pheromone composition. Pheromone production on a per male basis was highest for individual males and the percentage of geranic acid in the blend was lowest for individual males. Male pepper weevils produced only extremely low amounts of pheromone when feeding on artificial diet; however, they produced very high amounts when on fresh peppers. Together, this information will be useful in designing better attractant lures for pepper weevils.

  3. Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes

    Directory of Open Access Journals (Sweden)

    Harinath Dwivedi

    2012-01-01

    Full Text Available Gout is a metabolic disorder due to the deposition of uric acid crystals within articular or periarticular tissues. Uricase (urate oxidase catalyzes the oxidation of less water soluble uric acid (7 mg/dl to a compound allantoin which is more water soluble(11g/L at 40°C resulting into the ease of excretion of uric acid. The objective of the work was to develop a new method for screening of microbes for uricase production and estimation of uricase thereof. This was achieved by utilizing the fact that uric acid dissolves on being acted upon by uricase. The proposed method is a novel, inexpensive, simple and sensitive technique for screening and estimation of uricase. Biomass and uricase production at different stages of microbial growth curve for the uricase producing microbe was studied. Effects of different medium components affecting uricase production by microbes were studied using Placket Burman statistical design. Addition of uric acid in the nutrient medium was found to be effective in increasing the uricase production by microbes growing in the medium.

  4. Innovation Barriers and Enablers that Affect Productivity in Uganda Building Industry

    Directory of Open Access Journals (Sweden)

    Henry Mwanaki Alinaitwe, , and

    2007-06-01

    Full Text Available The construction industry has of recent been blamed for lack of innovation. Lack of innovation in the industry is believed to be responsible for the decreasing or stagnant levels of productivity in comparison with other industries. This paper reviews the major barriers and enablers to innovation in general. Propositions were made about the factors that affect innovation in the construction industry which were then formulated into a questionnaire. A survey was made on building contractors in Uganda, a developing country, targeting those with financial strength, large in size, and with high capacity to carry out big projects. The identified factors were then ranked and correlated. The level of training in science, engineering and technical education, and the level of research and development at the industry level are looked at as the greatest innovation enablers in building that will drive forward labour productivity. The size of the domestic market and the level of security are the worst innovation barriers that lead to low productivity in the building industry in Uganda. Contractors, policy makers and the government should address the identified factors in order to improve productivity.

  5. Factors affecting the milk production of dairy cattle in northern rural areas of Bangladesh

    Directory of Open Access Journals (Sweden)

    M.R. Begum

    2014-12-01

    Full Text Available A cross sectional study was conducted to observe the factors affecting the productive performance of dairy cattle from northern rural areas of Bangladesh during July and September 2013. Data of 105 cows, 85 (80.95% from local and 20 (19.05% cows from cross breed, were randomly selected for the study. A binary logistic regression, expressed by odds ratio with 95% confidence interval, was done to determine the association of daily milk production categorized into ≤ 2 and > 2 liters (L, based on median, with the significant explanatory variables of body weight, age at first calving, lactation period, vitamin use, type of floor and milking person. The result demonstrated that the probability of milk production of >2 L was 6.16, 4.5, 20.65 and 5.7 times higher from the with animal body weight of >140 kg, age at first calving of >36 m, lactation period of >8 m and vitamin use than that of body weight of 140 kg, age at first calving of ≤36 m, lactation period of ≤ 8 m, and not vitamin used respectively. The chance of milk production of > 2 L was 0.25 and 0.22 times lower for mud floor, and owner milking than that of brick floor and gowala (professional milking person respectively.

  6. Factors affecting exocellular polysaccharide production by Lactobacillus delbrueckii subsp. bulgaricus grown in a chemically defined medium.

    Science.gov (United States)

    Petry, S; Furlan, S; Crepeau, M J; Cerning, J; Desmazeaud, M

    2000-08-01

    We developed a chemically defined medium (CDM) containing lactose or glucose as the carbon source that supports growth and exopolysaccharide (EPS) production of two strains of Lactobacillus delbrueckii subsp. bulgaricus. The factors found to affect EPS production in this medium were oxygen, pH, temperature, and medium constituents, such as orotic acid and the carbon source. EPS production was greatest during the stationary phase. Composition analysis of EPS isolated at different growth phases and produced under different fermentation conditions (varying carbon source or pH) revealed that the component sugars were the same. The EPS from strain L. delbrueckii subsp. bulgaricus CNRZ 1187 contained galactose and glucose, and that of strain L. delbrueckii subsp. bulgaricus CNRZ 416 contained galactose, glucose, and rhamnose. However, the relative proportions of the individual monosaccharides differed, suggesting that repeating unit structures can vary according to specific medium alterations. Under pH-controlled fermentation conditions, L. delbrueckii subsp. bulgaricus strains produced as much EPS in the CDM as in milk. Furthermore, the relative proportions of individual monosaccharides of EPS produced in pH-controlled CDM or in milk were very similar. The CDM we developed may be a useful model and an alternative to milk in studies of EPS production.

  7. Human reproductive issues in space

    Science.gov (United States)

    Santy, Patricia A.; Jennings, Richard T.

    1992-01-01

    A review of reproductive functioning in animal species studied during space flight demonstrated that most species were affected significantly by the absence of gravity and/or the presence of radiation. These two factors induced alterations in normal reproductive functioning independently of, as well as in combination with, each other. Based on animal models, several potential problem areas regarding human reproductive physiology and functioning in the space environment were identified. While there are no current space flight investigations, the animal studies suggest priorities for future research in human reproduction. Such studies will be critical for the successful colonization of the space frontier.

  8. Colostrum and milk production of sows is affected by dietary conjugated linoleic acid

    DEFF Research Database (Denmark)

    Krogh, Uffe; Flummer, Christine; Jensen, Søren Krogh;

    2012-01-01

    (cis-9, trans-11 and trans-10, cis-12) from day 108 of gestation until weaning (4 wk after parturition) to evaluate whether dietary CLA affects the yield and composition of colostrum, time for initiation of milk production, and sow milk yield. Sows fed CLA tended to produce more colostral fat (6.3 vs...... was more than double in the CLA group (17.6 vs. 7.8%, respectively; P = 0.04). Copious milk production was initiated 33 h (CLA) and 34 h (CON) after parturition and was not affected by dietary treatments (P = 0.41). Sow milk yield was improved by the CLA treatment from days 7 to 14 of lactation (P = 0.......03). Weight at birth (1.40 kg for both groups; P = 0.98) and at weaning [8.2 kg (CLA) and 8.0 kg (CON); P = 0.52] was not statistically different. In conclusion, colostrum yield was inhibited but milk yield was stimulated by dietary inclusion of cis-9, trans-11 and trans-10, cis-12 CLA and indicates that sow...

  9. Mutations affecting extracellular protease production in the filamentous fungus Aspergillus nidulans.

    Science.gov (United States)

    Katz, M E; Flynn, P K; vanKuyk, P A; Cheetham, B F

    1996-04-10

    The extracellular proteases of Aspergillus nidulans are known to be regulated by carbon, nitrogen and sulphur metabolite repression. In this study, a mutant with reduced levels of extracellular protease was isolated by screening for loss of halo production on milk plates. Genetic analysis of the mutant showed that it contains a single, recessive mutation, in a gene which we have designated xprE, located on chromosome VI. The xprE1 mutation affected the production of extracellular proteases in response to carbon, nitrogen and, to a lesser extent, sulphur limitation. Three reversion mutations, xprF1, xprF2 and xprG1, which suppress xprE1, were characterised. Both xprF and xprG map to chromosome VII but the two genes are unlinked. The xprF1, xprF2 and xprG1 mutants showed high levels of milk-clearing activity on medium containing milk as a carbon source but reduced growth on a number of nitrogen sources. Evidence is presented that the xprE1 and xprG1 mutations alter expression of more than one protease and affect levels of alkaline protease gene mRNA.

  10. Efeito do pai do feto sobre as características produtivas e reprodutivas de vacas da raça pitangueiras Effect of the sire of fetus on productive and reproductive traits of cow from pitangueiras breed

    Directory of Open Access Journals (Sweden)

    João Francisco Pereira Bastos

    1999-01-01

    Full Text Available Para estimar a magnitude do efeito do pai do feto (PDF sobre as características produtivas e reprodutivas em fêmeas da raça Pitangueiras, foram analisadas 2287 lactações de 618 vacas, filhas de 82 reprodutores e acasaladas com 68 touros. As análises estatísticas foram executadas usando-se o programa LSMLMW (Mixed Model Least Squares and Maximum Likelihood Computer Program. O PDF não influenciou a produção de leite na lactação subseqüente, porém seu efeito foi significativo para produção e porcentagem de gordura, período de serviço, período de gestação e intervalo de partos. As estimativas dos efeitos do PDF, expressas como porcentagem da variância total foram: 0,81; 1,67; e 6,43%, respectivamente, para produção de leite e produção e porcentagem de gordura. Esta contribuição para as características reprodutivas foi estimada em 7,76% para o período de serviço; 1,23% para o período de gestação; e 7,57% para o intervalo de partos. As implicações genéticas do efeito do PDF sobre as características produtivas foram de pequena importância econômica, contudo, no desempenho reprodutivo, este efeito foi significativo e mensurável.To estimate the magnitude of sire of fetus (SOF effects on productive and reproductive traits in females of Pitangueiras breed, 2287 lactations of 618 cows, daughters of 82 bulls, mated with 68 sires were analyzed. The statistical analyses were made using LSMLMW program (Mixed Model Least Squares and Maximum Likelihood Computer Program. Sire of fetus (SOF did not affect the milk production in the subsequent lactation, however its effect was significant for the percent fat and production, days open, gestation length and calving intervals. The estimates of SOF effects, expressed as a percentage of total variance, were .81, 1.67 and 6.43% for milk and percent fat and yield, respectively. This contribution on the reproductive traits was estimated in 7.76% for days open, 1.23% for gestation

  11. A review of factors affecting productivity of bald eagles in the Great Lakes region: implications for recovery.

    Science.gov (United States)

    Bowerman, W W; Giesy, J P; Best, D A; Kramer, V J

    1995-05-01

    The bald eagle (Haliaeetus leucocephalus) population in North America declined greatly after World War II due primarily to the eggshell thinning effects of p,p'-DDE, a biodegradation product of DDT. After the banning of DDT in the United States and Canada during the early 1970s, the bald eagle population started to increase. However, this population recovery has not been uniform. Eagles nesting along the shorelines of the North American Great Lakes and rivers open to spawning runs of anadromous fishes from the Great Lakes still exhibit impaired reproduction. We have explored both ecological and toxicological factors that would limit reproduction of bald eagles in the Great Lakes region. Based on our studies, the most critical factors influencing eagle populations are concentrations of environmental toxicants. While there might be some continuing effects of DDE, total PCBs and most importantly 2,3,7,8-tetrachlordibenzo-p-dioxin equivalents (TCDD-EQ) in fishes from the Great Lakes and rivers open to spawning runs of anadromous fishes from the Great Lakes currently represent a significant hazard to bald eagles living along these shorelines or near these rivers and are most likely related to the impaired reproduction in bald eagles living there.

  12. Reproductive science as an essential component of conservation biology.

    Science.gov (United States)

    Holt, William V; Brown, Janine L; Comizzoli, Pierre

    2014-01-01

    In this chapter we argue that reproductive science in its broadest sense has never been more important in terms of its value to conservation biology, which itself is a synthetic and multidisciplinary topic. Over recent years the place of reproductive science in wildlife conservation has developed massively across a wide and integrated range of cutting edge topics. We now have unprecedented insight into the way that environmental change affects basic reproductive functions such as ovulation, sperm production, pregnancy and embryo development through previously unsuspected influences such as epigenetic modulation of the genome. Environmental change in its broadest sense alters the quality of foodstuffs that all animals need for reproductive success, changes the synchrony between breeding seasons and reproductive events, perturbs gonadal and embryo development through the presence of pollutants in the environment and drives species to adapt their behaviour and phenotype. In this book we explore many aspects of reproductive science and present wide ranging and up to date accounts of the scientific and technological advances that are currently enabling reproductive science to support conservation biology.

  13. VARIATIONS IN REPRODUCTIVE TOXICANT IDENTIFICATION

    Energy Technology Data Exchange (ETDEWEB)

    Simmons, F

    2008-05-13

    Reproductive toxicants are a very important class of compounds. They present unique hazards to those of child bearing ages, perform their 'dirty work' using a wide variety of mechanisms on a number of different organs, and are regulatorily important. Because of all of this, properly identifying reproductive toxicants is important, but fraught with difficulty. In this paper we will describe types or reproductive toxicants, their importance, and both mistakes and good practices that people who are not experts in reproductive toxicology may use in their attempts to identify them. Additionally, this paper will focus on chemical reproductive toxicants and will not address biological agents that could affect reproductive toxicity although many principles outlined here could be applied to that endeavor.

  14. Both Nsp1β and Nsp11 are responsible for differential TNF-α production induced by porcine reproductive and respiratory syndrome virus strains with different pathogenicity in vitro.

    Science.gov (United States)

    He, Qing; Li, Yan; Zhou, Lei; Ge, Xinna; Guo, Xin; Yang, Hanchun

    2015-04-02

    Porcine reproductive and respiratory syndrome virus (PRRSV) has been recognized to be one of the most important pathogens severely affecting global swine industry. An increasingly number of studies have paid much attention to the diverse roles of its nonstructural proteins (Nsps) in regulating the innate immune response of host upon PRRSV infection. In the present study, we first discovered that highly pathogenic PRRSV (HP-PRRSV) and low pathogenic PRRSV (LP-PRRSV) infection exhibited a differential TNF-α expression in pulmonary alveolar macrophages (PAMs), showing that HP-PRRSV infection induces lower TNF-α production at protein level in PAMs, compared with LP-PRRSV. Next, HP-PRRSV was confirmed to strongly suppress TNF-α production by inhibiting ERK signaling pathway. Finally, both Nsp1β and Nsp11 were demonstrated to be responsible for the inhibitory effect on TNF-α production induced by HP-PRRSV and the differential TNF-α production in PAMs. These findings contribute to the understanding of the pathogenesis of the Chinese HP-PRRSV.

  15. Different types of postpartum luteal activity affected by the exposure of heat stress and subsequent reproductive performance in Holstein lactating cows.

    Science.gov (United States)

    Kornmatitsuk, B; Chantaraprateep, P; Kornmatitsuk, S; Kindahl, H

    2008-10-01

    The aim of the present study was to determine the effect of heat stress on postpartum (PP) luteal activity and subsequent reproductive performance in lactating cows. Thirty Holstein-Friesian (HF, >or=75%) cows (hot season, n = 15 and cool season, n = 15) were studied over 60 days after calving. The changes of temperature-humidity index (THI) were monitored within both seasons. Body condition scores (BCS), uterine involution and the ovarian structures were recorded. Plasma samples were obtained three times a week for the analysis of progesterone (P4) and prostaglandin F(2 alpha) metabolite (PG metabolite). Subsequent reproductive performance of the cows, calved within the same period was also evaluated. The means of THI for hot and cool seasons varied between 84-87 and 78-83 respectively. A drop of the mean BCS was recorded at 5 weeks PP in the cows during hot season (p cows with normal PP ovarian cyclicity during hot and cool seasons were 4/15 (26.7%) and 9/15 (60.0%) respectively. A higher percentage of abnormal luteal activity was found in the cows during hot season (p = 0.07) and delayed luteal cyclicity/anovulation was the most pronounced atypical P4 profile. The levels of PG metabolite were not different between groups and the relationship between the levels of PG metabolite and the time of uterine involution was not evident (p > 0.05). The heat detection rates and the pregnancy rates were higher in the cows during cool season (p heat stress conditions had negative effects on BCS and altered a normal process of ovarian resumption PP, consequently resulted in lower reproductive performance in a tropical dairy herd.

  16. Dietary PUFA Intervention Affects Fatty Acid- and Micronutrient Profiles of Beef and Related Beef Products

    Directory of Open Access Journals (Sweden)

    Dirk Dannenberger

    2013-07-01

    Full Text Available The study investigated the dietary impact of 18:3n-3 vs. 18:2n-6 on fatty acid- and micronutrient concentration of beef muscle and the extent of diet- and processing-induced changes of lipid- and micronutrient concentrations of beef products made thereof (German Corned beef (GCB, tea sausage spread (TSS, scalded sausage (SS. Beef and beef products were obtained from German Holstein bulls which either received a control diet consisting of maize silage and concentrate with soybean meal (41%, or an experimental diet of grass silage and concentrate plus rapeseed cake (12% and linseed oil (3%. The study revealed that upon an 18:3n-3 vs. 18:2n-6 intervention the amounts of 18:3n-3, EPA and Σn-3 LC-PUFA were significantly increased by 2.6, 2.3 and 1.7 fold, respectively. Experimental diet significantly increased β-carotene contents, and the γ-tocopherol contents were decreased. During beef processing, n-3 PUFA from beef were found to be product-specifically transferred into the corresponding beef products. 18:3n-3 and Σn-3 LC-PUFA contents were found to be 1.4 and 1.5 times higher in GCB from grass silage- than maize silage-fed bulls. The trace element contents in GCB (iron, copper, zinc, selenium were not affected by the diet; however γ-tocopherol contents were decreased by experimental diet. In conclusion, dietary n-3 PUFA were completely transferred into beef products unaffected by beef processing conditions.

  17. New product, familiar taste: Effects of slogans on cognitive and affective responses to an unknown food product among food neophobics and neophilics

    NARCIS (Netherlands)

    Fenko, A.; Leufkens, Jean-Marie; Hoof, van J.J.

    2015-01-01

    Food neophobia is considered a potential barrier for the introduction of new food products. This study investigated how advertising slogans could influence cognitive and affective responses to a new product in food neophobics and food neophilics. An unknown dairy product was used to examine the effe

  18. Effect of prepubertal and postpubertal growth and age at first calving on production and reproduction traits during the first 3 lactations in Holstein dairy cattle.

    Science.gov (United States)

    Krpálková, L; Cabrera, V E; Vacek, M; Stípková, M; Stádník, L; Crump, P

    2014-05-01

    The objective of this study was to evaluate the effect of body condition score (BCS), body weight (BW), average daily weight gain (ADG), and age at first calving (AFC) of Holstein heifers on production and reproduction parameters in the 3 subsequent lactations. The data set consisted of 780 Holstein heifers calved at 2 dairy farms in the Czech Republic from 2007 to 2011. Their BW and BCS were measured at monthly intervals during the rearing period (5 to 18 mo of age), and the milk production and reproduction data of the first 3 lactations were collected over an 8-yr period (2005 to 2012). The highest milk yield in the first lactation was found in the group with medium ADG (5 to 14 mo of age; 0.949 to 0.850 kg of ADG). The highest average milk yield over lifetime performance was detected in heifers with the highest total ADG (≥ 0.950 kg/d). The difference in milk yield between the evaluated groups of highest ADG (in total and postpubertal growth ≥ 0.950 kg/d and in prepubertal growth ≥ 0.970 kg/d) and the lowest ADG (≤ 0.849 kg/d) was approximately 1,000 kg/305 d per cow. The highest milk yield in the first lactation was found in the group with the highest AFC ≥ 751 d, for which fat and protein content in the milk was not reduced. Postpubertal growth (11 to 14 mo of age) had the greatest effect on AFC. The group with lowest AFC ≤ 699 d showed a negative effect on milk yield but only in the first 100 d of the first parity. The highest ADG was detrimental to reproduction parameters in the first lactation. The highest BW at 14 mo (≥ 420 kg) led to lower AFC. Groups according to BCS at 14 mo showed no differences in AFC or milk yield in the first lactation or lifetime average production per lactation. We concluded that low AFC ≤ 699 d did not show a negative effect on subsequent production and reproduction parameters. Therefore, a shorter rearing period is recommended for dairy herds with suitable management.

  19. Nitrogen and phosphorus fertilization negatively affects strigolactone production and exudation in sorghum.

    Science.gov (United States)

    Yoneyama, Kaori; Xie, Xiaonan; Kisugi, Takaya; Nomura, Takahito; Yoneyama, Koichi

    2013-11-01

    Strigolactones (SLs) are essential host recognition signals for both root parasitic plants and arbuscular mycorrhizal fungi, and SLs or their metabolites function as a novel class of plant hormones regulating shoot and root architecture. Our previous study indicated that nitrogen (N) deficiency as well as phosphorus (P) deficiency in sorghum enhanced root content and exudation of 5-deoxystrigol, one of the major SLs produced by sorghum. In the present study, we examined how N and P fertilization affects SL production and exudation in sorghum plants subjected to short- (5 days) or long-term (10 days) N or P deficiency and demonstrated their common and distinct features. The root contents and exudation of SLs in the N- or P-deficient sorghum plants grown for 6, 12 or 24 h with or without N or P fertilization were quantified by LC-MS/MS. In general, without fertilization, root contents and exudation of SLs stayed at similar levels at 6 and 12 h and then significantly increased at 24 h. The production of SLs responded more quickly to P fertilization than the secretion of SLs, while regulation of SL secretion began earlier after N fertilization. It is suggested that sorghum plants regulate SL production and exudation when they are subjected to nutrient deficiencies depending on the type of nutrient and degree of deficiency.

  20. El bienestar animal en la reproducción y producción de cerdos - The animal welfare in the reproduction and production of pigs

    Directory of Open Access Journals (Sweden)

    Alejandro Córdova Izquierdo

    2007-12-01

    animals to live under the best possible conditions, without suffering unnecessary physical or psychological sufferings. For all the animal ones and especially for those whose destination will be to serve from source of foods to the man, the ethical commitment is intensified of offering them along its productive life the best conditions possible of habitat, sanity, handling, feeding and cares in general. At the present time,concepts of animal well-being, are question of complex and multifaceted public interest that includes important scientific, ethical, economic and political dimensions. To be a topic ofgrowing importance in the society, the animal welfare must approach nowadays on true scientific bases. The causes of the problems of animal welfare, are due to the erroneous perception about the animals, as beings that don't feel and that therefore, they are not able to suffer. It is easy that negative attitudes are developed toward the animals, that which isreflected in behaviors of negligence, cruelty or disrespectful treatment. The producers, veterinary doctors, as well as the society in general, concientes of the care of the animals,knows the importance about knowing the aspects of the comfort of the animals since the physiology, the development and the behavior of the animal they are affected by the bad environmental conditions, of production and of handling in general. In this review, aspects related with the animal welfares are presented in the reproduction and production of pigs.

  1. Avian reproductive physiology

    Science.gov (United States)

    Gee, G.F.; Gibbons, Edward F.; Durrant, Barbara S.; Demarest, Jack

    1995-01-01

    Knowledge of the many physiological factors associated with egg production , fertility, incubation, and brooding in nondomestic birds is limited. Science knows even less about reproduction in most of the 238 endangered or threatened birds. This discussion uses studies of nondomestic and, when necessary, domestic birds to describe physiological control of reproduction. Studies of the few nondomestic avian species show large variation in physiological control of reproduction. Aviculturists, in order to successfully propagate an endangered bird, must understand the bird's reproductive peculiarities. First, investigators can do studies with carefully chosen surrogate species, but eventually they need to confirm the results in the target endangered bird. Studies of reproduction in nondomestic birds increased in the last decade. Still, scientists need to do more comparative studies to understand the mechanisms that control reproduction in birds. New technologies are making it possible to study reproductive physiology of nondomestic species in less limiting ways. These technologies include telemetry to collect information without inducing stress on captives (Howey et al., 1987; Klugman, 1987), new tests for most of the humoral factors associated with reproduction, and the skill to collect small samples and manipulate birds without disrupting the physiological mechanisms (Bercovitz et al., 1985). Managers are using knowledge from these studies to improve propagation in zoological parks, private and public propagation facilities, and research institutions. Researchers need to study the control of ovulation, egg formation, and oviposition in the species of nondomestic birds that lay very few eggs in a season, hold eggs in the oviduct for longer intervals, or differ in other ways from the more thoroughly studied domestic birds. Other techniques that would enhance propagation for nondomestlc birds include tissue culture of cloned embryonic cells, cryopreservation of embryos

  2. Thiazide diuretics affect osteocalcin production in human osteoblasts at the transcription level without affecting vitamin D3 receptors.

    Science.gov (United States)

    Lajeunesse, D; Delalandre, A; Guggino, S E

    2000-05-01

    Besides their natriuretic and calciuretic effect, thiazide diuretics have been shown to decrease bone loss rate and improve bone mineral density. Clinical evidence suggests a specific role of thiazides on osteoblasts, because it reduces serum osteocalcin (OC), an osteoblast-specific protein, yet the mechanisms implicated are unknown. We therefore investigated the role of hydrochlorothiazide (HCTZ) on OC production by the human osteoblast-like cell line MG-63. HCTZ dose-dependently (1-100 microM) inhibited 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]-induced OC release by these cells (maximal effect, -40-50% and p ethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) only partly prevented the inhibitory effect of the diuretic on OC secretion (maximal effect, -22.5+/-6.9%), suggesting that thiazide-dependent Ca2+ influx is not sufficient to elicit the inhibition of OC secretion. Because OC production is strictly dependent on the presence of 1,25(OH)2D3 in human osteoblasts, we next evaluated the possible role of HCTZ on vitamin D3 receptors (VDR) at the mRNA and protein levels. Both Northern and Western blot analyses showed no effect of HCTZ (1-100 microM) on VDR levels. The presence of EGTA in the culture media reduced slightly the VDR mRNA levels under basal condition but this was not modified in the presence of increasing levels of HCTZ. The OC gene promoter also is under the control of transcription factors such as Yin Yang 1 (YY1) and cFOS. Western blot analysis revealed no changes in YY1 levels in response to HCTZ either in the presence or in the absence of 0.5 mM EGTA in the culture media. In contrast, HCTZ induced a dose-dependent increase in cFOS levels (p production by HCTZ could explain its preventive role in bone loss rate.

  3. Reproductive health.

    Science.gov (United States)

    1999-04-01

    This article explores the reproductive health status of China. Since 1990, China has stepped up its efforts in promoting reproductive health and maternal and child health. Several studies demonstrated a remarkable progress made in this area. By 1997, maternal and infant mortality rates have declined, while the penetration rate for the immunization program and inpatient delivery rate increased. Despite these achievements, however, much remains to be done such as the lack of client-centered approaches to meet the increasingly diverse needs of the population for family planning services. A survey conducted in 1995 showed that the country's family planning program was focused primarily on demographic issues while little attention was given to reproductive health objectives. The situation improved when the State Planning Commission implemented its pilot program called the Quality of Care in Family Planning in China. The program yielded encouraging results including a reoriented philosophy towards reproductive health services, enhanced service facilities, informed choices for family planning methods, and the development of an operational information system. Another strategy adopted to address fertility and reproductive health issues was the implementation of adolescent reproductive health education as a required course for senior middle schools. Lastly, this article provided a brief overview of China's HIV/AIDS situation.

  4. Phthalates as developmental reproductive toxicants

    Science.gov (United States)

    PE are a large family ofcompounds used in a wide array ofconsumer, industrial and medical products. Studies have shown that in utero treatment with PE such as diethyl hexyl phthalate (DEHP) during the critical period offetal reproductive development produced male reproductive mal...

  5. The sensory quality of meat, game, poultry, seafood and meat products as affected by intense light pulses: a systematic review

    OpenAIRE

    2015-01-01

    The effect of intense light pulses (ILP) on sensory quality of 16 different varieties of meat, meat products, game, poultry and seafood are reviewed. Changes induced by ILP are animal species, type of meat product and fluences applied dependent. ILP significantly deteriorates sensory quality of cooked meat products. It causes less change in the sensory properties of dry cured than cooked meat products while fermented sausage is least affected. The higher fluence applied significantly changes ...

  6. The receptor for advanced glycation end products (RAGE) affects T cell differentiation in OVA induced asthma.

    Science.gov (United States)

    Akirav, Eitan M; Henegariu, Octavian; Preston-Hurlburt, Paula; Schmidt, Ann Marie; Clynes, Raphael; Herold, Kevan C

    2014-01-01

    The receptor for glycation end products (RAGE) has been previously implicated in shaping the adaptive immune response. RAGE is expressed in T cells after activation and constitutively in T cells from patients with diabetes. The effects of RAGE on adaptive immune responses are not clear: Previous reports show that RAGE blockade affects Th1 responses. To clarify the role of RAGE in adaptive immune responses and the mechanisms of its effects, we examined whether RAGE plays a role in T cell activation in a Th2 response involving ovalbumin (OVA)-induced asthma in mice. WT and RAGE deficient wild-type and OT-II mice, expressing a T cell receptor specific for OVA, were immunized intranasally with OVA. Lung cellular infiltration and T cell responses were analyzed by immunostaining, FACS, and multiplex bead analyses for cytokines. RAGE deficient mice showed reduced cellular infiltration in the bronchial alveolar lavage fluid and impaired T cell activation in the mediastinal lymph nodes when compared with WT mice. In addition, RAGE deficiency resulted in reduced OT-II T cell infiltration of the lung and impaired IFNγ and IL-5 production when compared with WT mice and reduced infiltration when transferred into WT hosts. When cultured under conditions favoring the differentiation of T cells subsets, RAGE deficient T cells showed reduced production of IFNγ but increased production of IL-17. Our data show a stimulatory role for RAGE in T activation in OVA-induced asthma. This role is largely mediated by the effects of RAGE on T cell proliferation and differentiation. These findings suggest that RAGE may play a regulatory role in T cell responses following immune activation.

  7. An evaluation of fish early life stage tests for predicting reproductive and longer-term toxicity from plant protection product active substances.

    Science.gov (United States)

    Wheeler, James R; Maynard, Samuel K; Crane, Mark

    2014-08-01

    The chronic toxicity of chemicals to fish is routinely assessed by using fish early life stage (ELS) test results. Fish full life cycle (FLC) tests are generally required only when toxicity, bioaccumulation, and persistence triggers are met or when there is a suspicion of potential endocrine-disrupting properties. This regulatory approach is based on a relationship between the results of fish ELS and FLC studies first established more than 35 yrs ago. Recently, this relationship has been challenged by some regulatory authorities, and it has been recommended that more substances should undergo FLC testing. In addition, a project proposal has been submitted to the Organisation for Economic Cooperation and Development (OECD) to develop a fish partial life cycle (PLC) test including a reproductive assessment. Both FLC and PLC tests are animal- and resource-intensive and technically challenging and should therefore be undertaken only if there is clear evidence that they are necessary for coming to a regulatory decision. The present study reports on an analysis of a database of paired fish ELS and FLC endpoints for plant protection product active substances from European Union draft assessment reports and the US Environmental Protection Agency Office of Pesticide Programs Pesticide Ecotoxicity Database. Analysis of this database shows a clear relationship between ELS and FLC responses, with similar median sensitivity across substances when no-observed-effect concentrations (NOECs) are compared. There was also no indication that classification of a substance as a mammalian reproductive toxicant leads to more sensitive effects in fish FLC tests than in ELS tests. Indeed, the response of the ELS tests was generally more sensitive than the most sensitive reproduction NOEC from a FLC test. This analysis indicates that current testing strategies and guidelines are fit for purpose and that there is no need for fish full or partial life cycle tests for most plant protection

  8. Environmental factors influencing trace house gas production in permafrost-affected soils

    Science.gov (United States)

    Walz, Josefine; Knoblauch, Christian; Böhme, Luisa; Pfeiffer, Eva-Maria

    2016-04-01

    The permafrost-carbon feedback has been identified as a major feedback mechanism to climate change. Soil organic matter (SOM) decomposition in the active layer and thawing permafrost is an important source of atmospheric carbon dioxide (CO2) and methane (CH4). Decomposability and potential CO2 and CH4 production are connected to the quality of SOM. SOM quality varies with vegetation composition, soil type, and soil depth. The regulating factors affecting SOM decomposition in permafrost landscapes are not well understood. Here, we incubated permafrost-affected soils from a polygonal tundra landscape in the Lena Delta, Northeast Siberia, to examine the influence of soil depth, oxygen availability, incubation temperature, and fresh organic matter addition on trace gas production. CO2 production was always highest in topsoil (0 - 10 cm). Subsoil (10 - 50 cm) and permafrost (50 - 90 cm) carbon did not differ significantly in their decomposability. Under anaerobic conditions, less SOM was decomposed than under aerobic conditions. However, in the absence of oxygen, CH4 can also be formed, which has a substantially higher warming potential than CO2. But, within the four-month incubation period (approximate period of thaw), methanogenesis played only a minor role with CH4 contributing 1-30% to the total anaerobic carbon release. Temperature and fresh organic matter addition had a positive effect on SOM decomposition. Across a temperature gradient (1, 4, 8°C) aerobic decomposition in topsoil was less sensitive to temperature than in subsoil or permafrost. The addition of labile plant organic matter (13C-labelled Carex aquatilis, a dominant species in the region) significantly increased overall CO2 production across different depths and temperatures. Partitioning the total amount of CO2 in samples amended with Carex material into SOM-derived CO2 and Carex-derived CO2, however, revealed that most of the additional CO2 could be assigned to the organic carbon from the amendment

  9. Fine Mapping QTLs Affecting Milk Production Traits on BTA6 in Chinese Holstein with SNP Markers

    Institute of Scientific and Technical Information of China (English)

    LIU Rui; SUN Dong-xiao; WANG Ya-chun; YU Ying; ZHANG Yi; CHEN Hui-yong; ZHANG Qin; ZHANG Sheng-li; ZHANG Yuan

    2013-01-01

    Our previous studies demonstrated that the region around markers BMS470 and BMS1242 on BTA6 showed a linkage to 305-d milk yield and composition traits in the Chinese Holstein population. We herein focused on such narrow region to fine map milk production QTLs with 15 SNPs across 25 Mb with each SNP in 1 Mb within most regions in a Chinese Holstein population with daughter design. 1 449 Holstein cows and 11 sires were genotyped for such SNPs by using TaqMan probe and RFLP assays. Multipoint linkage analysis across family revealed a QTL affecting milk yield between PPARGC1A C4075T and SLC34A2 T1713C. Meanwhile, within family analysis found three milk yield QTLs (two in CR T60984131G-CEP135 C501T and one in PDLIM5 A106C-OPN T3907, a fat yield QTL in UGDH T1670C-CR T60984131G region, and two protein yield QTLs in TBC1D1 G501C-UGDH T1670C and PPARGC1A C4075T-SLC34A2 T1713C, respectively. Associations between aforementioned significant SNP markers and milk production traits were further implemented. We found significant associations of PPARGC1A C4075T, SLC34A2 T1713C with milk yield (P<0.05, P<0.01, P<0.01), UGDH T1670C, and CR T60984131G with fat yield (P<0.01, P<0.01), and PPARGC1A C4075T, SLC34A2 T1713C, UGDH T1670C and OPN T3907 with protein yield (P<0.01, P<0.01, P<0.01, P<0.01). Our findings implied that QTLs affecting milk production traits on BTA6 were pleictropism or multigenic effect and PPARGC1A and OPN may be the causal mutations behind milk production QTLs on BTA6 in the Chinese Holstein population.

  10. Decreased summer drought affects plant productivity and soil carbon dynamics in a Mediterranean woodland

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    M. F. Cotrufo

    2011-09-01

    Full Text Available Precipitation patterns are expected to change in the Mediterranean region within the next decades, with projected decreases in total rainfall and increases in extreme events. We manipulated precipitation patterns in a Mediterranean woodland, dominated by Arbutus unedo L., to study the effects of changing precipitation regimes on above-ground net primary production (ANPP and soil C dynamics, specifically plant-derived C input to soil and soil respiration (SR. Experimental plots were exposed to either a 20 % reduction of throughfall or to water addition targeted at maintaining soil water content above a minimum of 10 % v/v. Treatments were compared to control plots which received ambient precipitation. Enhanced soil moisture during summer months highly stimulated annual stem primary production, litter fall, SR and net annual plant-derived C input to soil which on average increased by 130 %, 26 %, 58 % and 220 %, respectively, as compared to the control. In contrast, the 20 % reduction in throughfall (equivalent to 10 % reduction in precipitation did not significantly change soil moisture at the site, and therefore did not significantly affect ANPP or SR. We conclude that minor changes (around 10 % reduction in precipitation amount are not likely to significantly affect ANPP or soil C dynamics in Mediterranean woodlands. However, if summer rain increases, C cycling will significantly accelerate but soil C stocks are not likely to be changed in the short-term. More studies involving modelling of long-term C dynamics are needed to predict if the estimated increases in soil C input under wet conditions is going to be sustained and if labile C is being substituted to stable C, with a negative effect on long-term soil C stocks.

  11. Quality of shrimp analogue product as affected by addition of modified potato starch.

    Science.gov (United States)

    Remya, S; Basu, S; Venkateshwarlu, G; Mohan, C O

    2015-07-01

    The present study was aimed to investigate the effects of addition of modified potato starch on the biochemical and textural properties of shrimp analogue/imitation shrimp, a popular value-added product prepared from surimi. Three batches of shrimp analogues were prepared with 0 % (NPS), 50 % (CPS) and 100 % (MPS) of modified starch incorporation and various quality attributes were monitored at regular intervals during frozen storage (-20 °C). Loss of myofibrillar protein was least for the shrimp analogue sample added with 100 % modified potato starch. The expressible moisture content of MPS (2.48 %) was less affected by long term storage compared to CPS (3.38 %) and NPS (3.99 %). During extended low temperature storage, the textural quality of sea food analogue was highly influenced by the type of starch added to it. The percentage of modified potato starch added to shrimp analogue significantly (p ≤ 0.05) affected its hardness and fracturability. MPS samples did not show significant changes in hardness during storage as compared to other two samples. Springiness of shrimp analogue increased 2.57, 1.5 and 1.77 times with the storage period for samples with NPS, CPS and MPS, respectively. Addition of modified potato starch improved the sensory quality and textural properties of shrimp analogue and reduced the quality degradation during frozen storage as compared to NPS which contained only native potato starch.

  12. Meta-analysis of the effect of immunocastration on production performance, reproductive organs and boar taint compounds in pigs.

    Science.gov (United States)

    Batorek, N; Čandek-Potokar, M; Bonneau, M; Van Milgen, J

    2012-08-01

    Meta-analytical approach was used to quantitatively synthesize the effect of immunocastration on growth, carcass, meat quality, reproductive organs and boar taint compounds. Altogether, 41 papers were collected for effect size (θ) calculation and the comparisons were made with entire males (EM) and surgical castrates (SC). The data for reproductive organs and growth performance are numerous enough to draw firm conclusions. In contrast, data for carcass and meat quality are more limited. Results of meta-analysis show efficient immunocastration with the magnitude of the response being by far the largest for reproductive organs (θ = -2.8 to -5.0) and boar taint substances (θ = -2.8 and -0.8 for androstenone and skatole, respectively). However, compared with SC, the immunocastrates exhibit larger bulbourethral glands (θ = 1.3) and slightly higher concentrations of androstenone and skatole (θ = 0.1 and θ = 0.2, respectively). The impact of immunocastration is also remarkable on performance, where the main advantage of the immunocastrates is their boar-like performance until revaccination. In the period following the second vaccination, they eat much more than EM (θ = 2.1), resulting in large effect size for growth rate compared with both EM and SC (θ = 1.1 and θ = 1.4, respectively). Considering the whole fattening period, their feed conversion ratio is higher compared with EM (θ = 0.6) and much lower than that of SC (θ = -1.3), although exhibiting moderately faster growth compared with both (θ = 0.6 and θ = 0.2, respectively). With regard to carcass quality, the immunocastrates take intermediate position between EM and SC. Besides, our analysis suggests no difference in meat quality with SC and some meat quality advantages of immunocastrates over EM because of higher intramuscular fat content (θ = 0.4) and lower shear force (θ = -0.6).

  13. The camp analogue, dbcAMP can stimulate rabbit reproductive functions: I. Effect on ovarian folliculogenesis, ovulation and embryo production

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    Chrenek P.

    2012-01-01

    Full Text Available The aim of our study was to examine the influence of administration of N6,2’-dibutyryladenosine 3’5’-cyclic monophosphate (dbcAMP, a cAMP agonist, on ovarian folliculogenesis and atresia, as well as on reproductive efficiency in rabbits, whose ovarian cycle and ovulation was induced by gonadotropins. Ovarian cycle and ovulation of control rabbits were induced by 20 IU/kg PMSG followed by 35 IU/kg hCG administration. Experimental animals received PMSG and hCG together with dbcAMP (at 5, 25 or 50 μg/animal. After ovulation and insemination, the animals were sacrificed. Ovaries were weighted, histological sections of ovaries were prepared, and the presence of ovulated and not ovulated follicles and different stages of atresia was evaluated by light microscopy. The eggs were flushed from the oviducts after insemination and cultured up to blastocyst cell stage. Numbers of ovarian Corpora lutea, ovulated oocytes and oocyte-derived zygotes and embryos reaching hatched blastocyst stage were determined. Administration of dbcAMP (at doses 25 or 50 μg/animal, but not at 5 μg/animal was able to increase the proportion of follicles with cystic and luteinization-related atresia. Furthermore, dbcAMP (50 μg/animal, but not lower doses increased the ovarian mass, number of Corpora lutea, number of harvested oocytes, zygotes and embryos at blastocyst stage derived from these zygotes after culture. These data demonstrate that dbcAMP can stimulate rabbit ovarian follicle atresia, ovulation, oocyte, zygote and embryo yield and development. Furthermore, they confirm in the involvement of cyclic nucleotide-dependent intracellular mechanisms in the control of rabbit reproductive functions and potential practical usefulness of dbcAMP in improving animal reproduction and fertility.

  14. Application of syndromic surveillance on routinely collected cattle reproduction and milk production data for the early detection of outbreaks of Bluetongue and Schmallenberg viruses.

    Science.gov (United States)

    Veldhuis, Anouk; Brouwer-Middelesch, Henriëtte; Marceau, Alexis; Madouasse, Aurélien; Van der Stede, Yves; Fourichon, Christine; Welby, Sarah; Wever, Paul; van Schaik, Gerdien

    2016-02-01

    This study aimed to evaluate the use of routinely collected reproductive and milk production data for the early detection of emerging vector-borne diseases in cattle in the Netherlands and the Flanders region of Belgium (i.e., the northern part of Belgium). Prospective space-time cluster analyses on residuals from a model on milk production were carried out to detect clusters of reduced milk yield. A CUSUM algorithm was used to detect temporal aberrations in model residuals of reproductive performance models on two indicators of gestation length. The Bluetongue serotype-8 (BTV-8) epidemics of 2006 and 2007 and the Schmallenberg virus (SBV) epidemic of 2011 were used as case studies to evaluate the sensitivity and timeliness of these methods. The methods investigated in this study did not result in a more timely detection of BTV-8 and SBV in the Netherlands and BTV-8 in Belgium given the surveillance systems in place when these viruses emerged. This could be due to (i) the large geographical units used in the analyses (country, region and province level), and (ii) the high level of sensitivity of the surveillance systems in place when these viruses emerged. Nevertheless, it might be worthwhile to use a syndromic surveillance system based on non-specific animal health data in real-time alongside regular surveillance, to increase the sense of urgency and to provide valuable quantitative information for decision makers in the initial phase of an emerging disease outbreak.

  15. Selection of barley grain affects ruminal fermentation, starch digestibility, and productivity of lactating dairy cows.

    Science.gov (United States)

    Silveira, C; Oba, M; Yang, W Z; Beauchemin, K A

    2007-06-01

    The objective of this study was to evaluate the effects of 2 lots of barley grain cultivars differing in expected ruminal starch degradation on dry matter (DM) intake, ruminal fermentation, ruminal and total tract digestibility, and milk production of dairy cows when provided at 2 concentrations in the diet. Four primiparous ruminally cannulated (123 +/- 69 d in milk; mean +/- SD) and 4 multiparous ruminally and duodenally cannulated (46 +/- 14 d in milk) cows were used in a 4 x 4 Latin Square design with a 2 x 2 factorial arrangement of treatments with 16-d periods. Primiparous and multiparous cows were assigned to different squares. Treatments were 2 dietary starch concentrations (30 vs. 23% of dietary DM) and 2 lots of barley grain cultivars (Xena vs. Dillon) differing in expected ruminal starch degradation. Xena had higher starch concentration (58.7 vs. 50.0%) and greater in vitro 6-h starch digestibility (78.0 vs. 73.5%) compared with Dillon. All experimental diets were formulated to supply 18.3% crude protein and 20.0% forage neutral detergent fiber. Dry matter intake and milk yield were not affected by treatment. Milk fat concentration (3.55 vs. 3.29%) was greater for cows fed Dillon compared with Xena, but was not affected by dietary starch concentration. Ruminal starch digestion was greater for cows fed high-starch diets compared with those fed low-starch diets (4.55 vs. 2.49 kg/d), and tended to be greater for cows fed Xena compared with those fed Dillon (3.85 vs. 3.19 kg/d). Ruminal acetate concentration was lower, and propionate concentration was greater, for cows fed Xena or high-starch diets compared with cows fed Dillon or low-starch diets, respectively. Furthermore, cows fed Xena or high-starch diets had longer duration that ruminal pH was below 5.8 (6.6 vs. 4.0 and 6.4 vs. 4.2 h/d) and greater total tract starch digestibility (94.3 vs. 93.0 and 94.3 vs. 93.0%) compared with cows fed Dillon or low-starch diets, respectively. These results

  16. Effects of main reproductive and health problems on the performance of dairy cows: a review

    Directory of Open Access Journals (Sweden)

    N. Ghavi Hossein-Zadeh

    2013-01-01

    Full Text Available This review focuses on the potential effects of twinning, dystocia, stillbirth, abortion, retained placenta and metritis on the productive and reproductive performances in dairy cattle. These are diverse disorders that are similar in that they all can result in impaired performance of dairy cows. Reproductive problems occur frequently in lactating dairy cows and can dramatically affect reproductive efficiency in a dairy herd. Poor reproductive performance is a major cause of involuntary culling and therefore reduces the opportunity for voluntary culling and has a negative influence on the subsequent productivity of a dairy herd. Reproductive performance is influenced by the interactive effect of environment, management, health, and genetic factors. In addition, diseases mainly affect dairy cow productivity by decreasing reproductive efficiency, shortening the expected length of productive life and by lowering milk production. Deciding whether to breed, treat, or cull dairy cows showing one or more of these problems is a challenge for both veterinarians and dairy producers. In addition, there is considerable debate among dairy scientists and bovine practitioners regarding the economic impact of these problems in a dairy operation and the most effective management or therapeutic intervention for treating them. Because of this controversy, dairy managers should focus on prevention and control of risk factors associated with each problem rather than on prescriptive therapeutic interventions.

  17. Factors affecting wild rabbit production in extensive breeding enclosures: how can we optimise efforts?

    Directory of Open Access Journals (Sweden)

    José Guerrero-Casado

    2013-09-01

    Full Text Available The declining rabbit population in the Iberian Peninsula has led hunters and authorities to rear rabbits in captivity systems for their subsequent release. One alternative method to intensive rabbitry systems is the use of extensive breeding enclosures, since they produce animals of greater quality for hunting and conservation purposes. However, some of the factors that affect rabbit production in breeding enclosures are still unknown. The present study used partial least squares regression (PLSR to analyse the effects of plot size, scrub cover, slope, initial rabbit abundance, the resources needed to dig warrens, predation and proximity to other enclosures on rabbit abundance. The results of our study show a positive effect of the number of other fenced plots within a radius of 3 km, a positive relationship with the availability of optimal resources for building warrens and a positive influence of intermediate values of scrub cover. According to our results, to maximise rabbit production in the enclosures it would be advisable to concentrate the restocking effort by ensuring that the restocking plots are close to each other, thus avoiding isolated enclosures. Furthermore, the selection of plots with an appropriate scrub cover and high availability of elements that favour the construction of warrens, such as large stones, sloping land or tall shrubs, may optimise results.

  18. Klebsiella pneumoniae yfiRNB operon affects biofilm formation, polysaccharide production and drug susceptibility.

    Science.gov (United States)

    Huertas, Mónica G; Zárate, Lina; Acosta, Iván C; Posada, Leonardo; Cruz, Diana P; Lozano, Marcela; Zambrano, María M

    2014-12-01

    Klebsiella pneumoniae is an opportunistic pathogen important in hospital-acquired infections, which are complicated by the rise of drug-resistant strains and the capacity of cells to adhere to surfaces and form biofilms. In this work, we carried out an analysis of the genes in the K. pneumoniae yfiRNB operon, previously implicated in biofilm formation. The results indicated that in addition to the previously reported effect on type 3 fimbriae expression, this operon also affected biofilm formation due to changes in cellulose as part of the extracellular matrix. Deletion of yfiR resulted in enhanced biofilm formation and an altered colony phenotype indicative of cellulose overproduction when grown on solid indicator media. Extraction of polysaccharides and treatment with cellulase were consistent with the presence of cellulose in biofilms. The enhanced cellulose production did not, however, correlate with virulence as assessed using a Caenorhabditis elegans assay. In addition, cells bearing mutations in genes of the yfiRNB operon varied with respect to the WT control in terms of susceptibility to the antibiotics amikacin, ciprofloxacin, imipenem and meropenem. These results indicated that the yfiRNB operon is implicated in the production of exopolysaccharides that alter cell surface characteristics and the capacity to form biofilms--a phenotype that does not necessarily correlate with properties related with survival, such as resistance to antibiotics.

  19. Developmental and communicative factors affecting VOT production in English and Arabic bilingual and monolingual speakers

    Science.gov (United States)

    Khattab, Ghada

    2001-05-01

    VOT patterns were investigated in the production of three Lebanese-English bilinguals' aged 5, 7, and 10, six aged-matched monolingual controls from the bilinguals' immediate communities, and the parents of bilinguals and monolinguals. The aim was to examine the extent to which children exposed to two languages acquire separate VOT patterns for each language and to determine the factors that affect such acquisition. Results showed that VOT patterns for each bilingual child differed significantly across the two languages. But while the contrast in English resembled a monolingual-like model, that for Arabic exhibited persisting developmental features; explanations were offered in terms of the relationship between input and complexity of voicing lead production. Evidence was used from developmental changes that were noted for two of the bilingual subjects over a period of 18 months. English code-switches produced by the bilinguals during Arabic sessions exhibited different VOT patterns from those produced during English sessions, which underlined the importance of taking the language context into consideration. Finally, results from monolinguals and bilinguals showed that the short lag categories for the two languages were different despite a degree of overlap. Such findings require finer divisions of the three universal VOT categories to account for language-specific patterns.

  20. Optimization of factors affecting the production of biodiesel from crude palm kernel oil and ethanol

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    David K. Kuwornoo, Julius C. Ahiekpor

    2010-07-01

    Full Text Available Biodiesel, an alternative diesel fuel made from renewable sources such as vegetable oils and animal fats, has been identified by government to play a key role in the socio-economic development of Ghana. The utilization of biodiesel is expected to be about 10% of the total liquid fuel mix of the country by the year 2020. Despite this great potential and the numerous sources from which biodiesel could be developed in Ghana, studies on the sources of biodiesel and their properties as a substitute for fossil diesel have tended to be limited to Jatropha oil. This paper, however, reports the parameters that influences the production of biodiesel from palm kernel oil, one of the vegetable oils obtained from oil palm which is the highest vegetable oil source in Ghana. The parameters studied are; mass ratio of ethanol to oil, reaction temperature, catalyst concentration, and reaction time using completely randomized 24 factorial design. Results indicated that ethanol to oil mass ratio, catalyst concentration and reaction time were the most important factors affecting the ethyl ester yield. There was also an interaction effect between catalyst and time and ethanol- oil ratio and time on the yield. Accordingly, the optimal conditions for the production of ethyl esters from crude palm kernel oil were determined as; 1:5 mass ratio of ethanol to oil, 1% catalyst concentration by weight of oil, 90 minutes reaction time at a temperature of 30 oC.

  1. Opposing effects of D-aspartic acid and nitric oxide on tuning of testosterone production in mallard testis during the reproductive cycle

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    Assisi Loredana

    2008-07-01

    Full Text Available Abstract Background D-Aspartic acid (D-Asp and nitric oxide (NO play an important role in tuning testosterone production in the gonads of male vertebrates. In particular, D-Asp promotes either the synthesis or the release of testosterone, whereas NO inhibits it. In this study, we have investigated for the first time in birds the putative effects of D-Asp and NO on testicular testosterone production in relation to two phases of the reproductive cycle of the adult captive wild-strain mallard (Anas platyrhynchos drake. It is a typical seasonal breeder and its cycle consists of a short reproductive period (RP in the spring (April-May and a non reproductive period (NRP in the summer (July, a time when the gonads are quiescent. The presence and the localization of D-Asp and NO in the testis and the trends of D-Asp, NO and testosterone levels were assessed during the main phases of the bird's reproductive cycle. Furthermore, in vitro experiments revealed the direct effect of exogenously administered D-Asp and NO on testosterone steroidogenesis. Methods By using immunohistochemical (IHC techniques, we studied the presence and the distributional pattern of D-Asp and NO in the testes of RP and NRP drakes. D-Asp levels were evaluated by an enzymatic method, whereas NO content, via nitrite, was assessed using biochemical measurements. Finally, immunoenzymatic techniques determined testicular testosterone levels. Results IHC analyses revealed the presence of D-Asp and NO in Leydig cells. The distributional pattern of both molecules was in some way correlated to the steroidogenic pathway, which is involved in autocrine testosterone production. Indeed, whereas NO was present only during the NRP, D-Asp was almost exclusively present during the RP. Consistently, the high testosterone testicular content occurring during RP was coupled to a high D-Asp level and a low NO content in the gonad. By contrast, in sexually inactive drakes (NRP, the low testosterone

  2. Trinitrotoluene and mandarin peels selectively affect lignin-modifying enzyme production in white-rot basidiomycetes.

    Science.gov (United States)

    Kachlishvili, Eva; Asatiani, Mikheil; Kobakhidze, Aza; Elisashvili, Vladimir

    2016-01-01

    Five white-rot basidiomycetes (WRB) species have been evaluated for their potential to tolerate and to degrade 0.2 mM 2, 4, 6-trinitrotoluene (TNT) as well as to produce laccase and manganese peroxidase (MnP) in presence of this xenobiotic. The tested fungal strains produced laccase in both glycerol and mandarin peels-containing media, whereas in the glycerol-containing medium only Cerrena unicolor strains and Trametes versicolor BCC 775 secreted MnP. Replacement of glycerol by milled mandarin peels 3- to 45-fold increased laccase activity, promoted C. unicolor strains and T. versicolor MnP secretion and induced this enzyme production by Fomes fomentarius BCC 38 and Funalia trogii BCC 146. Differential response of the WRB strains to the TNT addition was observed. In particular, laccase activity of C. unicolor increased 2- to 3-fold in both media whereas no stimulation of the laccase production was revealed in cultivation of F. fomentarius. TNT practically did not affect the MnP activity. Two strains of C. unicolor followed by T. versicolor producing laccase and MnP almost completely removed 0.2 mM TNT from the synthetic medium. Increase of TNT concentration from 0 to 0.4 mM in the mandarin peels-based medium and from 0 to 0.3 mM in the glycerol-containing medium stimulated C. unicolor BCC 300 laccase production from 92.4 to 240.7 U/ml and from 17.1 to 48.6 U/ml, respectively. This strain has been resistant to the TNT high concentration and has ability to remove 85 % of initial 0.3 mM TNT content during 6 days of the submerged cultivation.

  3. Soil hydraulic properties affected by topsoil thickness in cultivated switchgrass and corn-soybean rotation production systems

    Science.gov (United States)

    Loss of productive topsoil by soil erosion over time can reduce the productive capacity of soil and can significantly affect soil hydraulic properties. This study evaluated the effects of reduced topsoil thickness and perennial switchgrass (Panicum virgatum L.) versus corn (Zea mays L.)/soybean [Gly...

  4. Characterisation of TNF block haplotypes affecting the production of TNF and LTA.

    Science.gov (United States)

    Tan, J H; Temple, S E L; Kee, C; Waterer, G W; Tan, C R T; Gut, I; Price, P

    2011-02-01

    Polymorphisms in the central major histocompatibility complex (MHC) (particularly TNF and adjacent genes) associate with several immunopathological diseases and with susceptibility to pneumonia. The MHC is characterised by strong linkage disequilibrium (LD), so identification of loci affecting disease must be based on haplotypes. We have defined 31 tumour necrosis factor (TNF) block haplotypes (denoted FV1-31) in Caucasians, Asians and Australian Aboriginals. This study correlates the carriage of TNF block haplotypes with TNF and lymphotoxin alpha (LTA) protein production by peripheral blood mononuclear cells from 205 healthy Caucasian subjects, following in vitro stimulation with Streptococcus pneumoniae (S. pneumoniae; gram-positive bacteria), Escherichia coli (E. coli; gram-negative bacteria) or TNF over 4, 8 and 24 h. Fifteen haplotypes were present at >1%, accounting for 94.5% of the cohort. The haplotypes were grouped into five families based on common alleles. Following stimulation, cells from carriers of the FV10 haplotype (family 2) produced less LTA compared with non-FV10 carriers. Carriers of the FV18 haplotype (family 4) produced more LTA than other donors. Induction of TNF by S. pneumoniae following 24 h stimulation was also greater in donors with FV18. The FV18 haplotype associated with the 44.1 MHC ancestral haplotype (HLA-A2, -C5, -B44, -DRB1*0401 and -DQB1*0301) that has few disease associations. FV16 occurred in the 8.1 MHC haplotype (HLA-A2, B8, DR3) that is associated with multiple immunopathological diseases. FV16 did not affect TNF or LTA levels. The findings suggest that many genetic variations critical in vivo are not effectively modelled by short-term cultures.

  5. Reproductive efficiency and metabolism of female broiler breeders as affected by genotype, feed allocation, and age at photostimulation. 1. Pullet growth and development.

    Science.gov (United States)

    Robinson, F E; Zuidhof, M J; Renema, R A

    2007-10-01

    A 3 x 4 x 2 factorial design was carried out to determine the effect of 3 broiler breeder strains, 4 target BW profiles, and 2 photostimulation ages on pullet growth and development. A total of 560 pullets from each strain (Hubbard Hi-Y, Ross 508, and Ross 708) were reared on BW profiles that separated at 4 wk and converged at 32 wk of age as follows: standard (mean target BW profile of the 3 strains used), low (12-wk BW target = 25% lower than standard followed by rapid gain to 32 wk), moderate (12-wk BW target = 150% of standard followed by lower rate of gain to 32 wk), and high (12-wk BW target = 200% of standard followed by minimal growth to 32 wk). Birds were photostimulated at 18 (18WK) or 22 wk (22WK). During the prephotostimulation phase (2 to 18 wk of age), 4 birds were killed for each of the 12 interactions at 14-d intervals to characterize changes in carcass traits. After 18 wk (wk 20, 22, and 24), 4 birds from each of the 24 interactions were killed and dissected (n = 768). Growth rate restricted frame size (e.g., 18-wk shank length: low = 101.8; standard = 105.6; moderate = 109.5; and high = 112.3 mm). At 24 wk of age, the 22WK birds had similar amounts of breast muscle compared with 18WK birds, whereas the later photostimulated hens had heavier abdominal fat pads. Early photo-stimulation resulted in increased 24-wk liver weights in all strains, but the difference was greatest in Ross 708 birds. The 22-wk ovary weight was influenced by age at photostimulation in high (18WK = 17.3; 22WK = 1.6 g) and moderate (18WK = 14.1; 22WK = 1.1 g) birds. The more extensive feed restriction of LOW birds before 12 wk of age appeared to limit breast muscle and fat pad growth and slow reproductive tract development following photostimulation. Pullets on heavier BW profiles respond to early PS by developing the reproductive system at the expense of breast muscle and fat pad growth. Genetic strain modulates some of the effect of very different target BW profiles.

  6. Body size and clonality consequences for sexual reproduction in a perennial herb of Brazilian rupestrian grasslands.

    Science.gov (United States)

    Demetrio, G R; Coelho, F F; Barbosa, M E A

    2014-08-01

    Body size is one of the most important factors regarding herbaceous perennial plants life-histories, and several fitness components of these organisms are related to size. Clonal plants show distinct kinds of reproduction and can develop offspring by sexual or asexual ways. We aimed to understand how body size affects Comanthera nivea (Eriocaulaceae) sexual reproduction and to verify how clonal growth is related to flower head production in this species. We sampled 600 rosettes in rupestrian grasslands and performed linear regression analysis between body size and number of produced flower heads. We also compared the flower head production between isolated rosettes and rosettes within clones. Our results showed that body size was significantly related, but explained only a small part of flower head production. The flower head production was higher in rosettes within clones than in isolated ones. The clones presented a rosette or a small group of rosettes that concentrated the sexual reproduction. Clonality was positively associated with sexual reproduction. Clonality can represent an important way of allowing the persistence of plants by sexual reproduction in markedly seasonal stressful environments. The cases of clonality enhancing the sexual reproduction must be considered and put in focus on reproductive biology research.

  7. Physical processes affecting availability of dissolved silicate for diatom production in the Arabian Sea

    Science.gov (United States)

    Young, David K.; Kindle, John C.

    1994-01-01

    A passive tracer to represent dissolved silicate concentrations, with biologically realistic uptake kinetics, is successfully incorporated into a three-dimensional, eddy-resolving, ocean circulation model of the Indian Ocean. Hypotheses are tested to evaluate physical processes which potentially affect the availability of silicate for diatom production in the Arabian Sea. An alternative mechanism is offered to the idea that open ocean upwelling is primarily responsible for the high, vertical nutrient flux and consequent large-scale phytoplankton bloom in the northwestern Arabian Sea during the southwest monsoon. Model results show that dissolved silicate in surface waters available for uptake by diatoms is primarily influenced by the intensity of nearshore upwelling from soutwest monsoonal wind forcing and by the offshore advective transport of surface waters. The upwelling, which in the model occurs within 200 +/- 50 km of the coast, appears to be a result of a combination of coastal upwelling, Elkman pumping, and divergence of the coastal flow as it turns offshore. Localized intensifications of silicate concentrations appear to be hydrodynamically driven and geographically correlated to coastal topographic features. The absence of diatoms in sediments of the eastern Arabian Basin is consistent with modeled distributional patterns of dissolved silicate resulting from limited westward advection of upwelled coastal waters from the western continental margin of India and rapid uptake of available silicate by diatoms. Concentrations of modeled silicate become sufficiently low to become unavailable for diatom production in the eastern Arabian Sea, a region between 61 deg E and 70 deg E at 8 deg N on the south, with the east and west boundaries converging on the north at approximately 67 deg E, 20 deg N.

  8. Denitrifying bacterial communities affect current production and nitrous oxide accumulation in a microbial fuel cell.

    Directory of Open Access Journals (Sweden)

    Ariadna Vilar-Sanz

    Full Text Available The biocathodic reduction of nitrate in Microbial Fuel Cells (MFCs is an alternative to remove nitrogen in low carbon to nitrogen wastewater and relies entirely on microbial activity. In this paper the community composition of denitrifiers in the cathode of a MFC is analysed in relation to added electron acceptors (nitrate and nitrite and organic matter in the cathode. Nitrate reducers and nitrite reducers were highly affected by the operational conditions and displayed high diversity. The number of retrieved species-level Operational Taxonomic Units (OTUs for narG, napA, nirS and nirK genes was 11, 10, 31 and 22, respectively. In contrast, nitrous oxide reducers remained virtually unchanged at all conditions. About 90% of the retrieved nosZ sequences grouped in a single OTU with a high similarity with Oligotropha carboxidovorans nosZ gene. nirS-containing denitrifiers were dominant at all conditions and accounted for a significant amount of the total bacterial density. Current production decreased from 15.0 A · m(-3 NCC (Net Cathodic Compartment, when nitrate was used as an electron acceptor, to 14.1 A · m(-3 NCC in the case of nitrite. Contrarily, nitrous oxide (N2O accumulation in the MFC was higher when nitrite was used as the main electron acceptor and accounted for 70% of gaseous nitrogen. Relative abundance of nitrite to nitrous oxide reducers, calculated as (qnirS+qnirK/qnosZ, correlated positively with N2O emissions. Collectively, data indicate that bacteria catalysing the initial denitrification steps in a MFC are highly influenced by main electron acceptors and have a major influence on current production and N2O accumulation.

  9. Survival and reproduction of some blue-green and green algae as affected by sewage water, fertilizer factory effluent, brassica oil, phenol, toluene and benzene.

    Science.gov (United States)

    Agrawal, S C; Gupta, S

    2009-01-01

    Fourteen blue-green and green algae survived for widely different time periods ranging between 22-102 d in control culture medium. Irrespective of their long or short survival period in control cultures, their pro- or eukaryotic nature, their different morphological types or natural habitats, they all survived for a short time period ranging between 3-8 d in sewage water, 5-10 d in fertilizer factory effluent, (1/4)-2 d in brassica oil, (1/2)-2 d in phenol, 1-3 d in toluene, and 1-4 d in benzene (showing the relative toxicity of different chemicals to different algae, and the antialgal nature of brassica oil). Dilution decreased the toxicity of these agents very little, indicating that they all were very toxic to algae. None of the agent induced the formation of any reproductive or dormant cells. Sewage water, fertilizer factory effluent, brassica oil and/or benzene favored the formation of necridia cells in Phormidium bohneri, P. foveolarum, Microcoleus chthonoplastes, Lyngbya birgei, and L. major filaments. Scenedesmus quadricauda shed off all spines earlier, Hormidium flaccidum fragmented less or not at all, Scytonema millei formed no false branch and heterocyst, Aphanothece pallida and Gloeocapsa atrata cells did not divide, Cosmarium granatum cells did not form any zygospore and Oedogonium sp. not any oogonia-like cells under all or most of treatments with 25-100 % sewage water, 1-100 % fertilizer factory effluent, 1-100 % brassica oil, 25-100 % phenol, toluene and benzene.

  10. The Other Side: How does Informed Choice Affect Induced Abortions among Reproductive-Age Immigrant Women in China—A Cross-Sectional Study

    Directory of Open Access Journals (Sweden)

    Chuanning Yu

    2016-10-01

    Full Text Available This study attempted to explore how informed choice on contraceptive methods influenced induced abortions among reproductive-age immigrant women in China. A total of 3230 participants were recruited in Beijing, Shanghai, and Chongqing. Information on informed choice was collected by questionnaires. The annual incidence rate (spells of induced abortions was 0.46 (1500/3230 among the participants. The sequence from the highest score to the lowest was long-term, short-term and natural contraceptive methods (p < 0.0001. Significant differences of rates in induced abortions were found in region, occupation, length of the first immigration up to now (year, purpose for immigration, number of children, marital status, sex preference, contraceptive methods, deciders of contraceptive methods and side effects. In the zero-inflated negative binomial model, the joint impacts showed when a participant with one child employed condoms or family planning service providers as the deciders of contraceptive methods introduced intrauterine devices, the occurrence of induced abortions was more likely to be reduced. Women who underwent side effects using pills were more likely to have had induced abortions.

  11. The Other Side: How does Informed Choice Affect Induced Abortions among Reproductive-Age Immigrant Women in China—A Cross-Sectional Study

    Science.gov (United States)

    Yu, Chuanning; Wu, Junqing; Li, Yuyan; Zhou, Ying; Zhao, Rui; Ji, Honglei; Li, Yi-Ran; Han, Ying; Tong, Qi

    2016-01-01

    This study attempted to explore how informed choice on contraceptive methods influenced induced abortions among reproductive-age immigrant women in China. A total of 3230 participants were recruited in Beijing, Shanghai, and Chongqing. Information on informed choice was collected by questionnaires. The annual incidence rate (spells) of induced abortions was 0.46 (1500/3230) among the participants. The sequence from the highest score to the lowest was long-term, short-term and natural contraceptive methods (p abortions were found in region, occupation, length of the first immigration up to now (year), purpose for immigration, number of children, marital status, sex preference, contraceptive methods, deciders of contraceptive methods and side effects. In the zero-inflated negative binomial model, the joint impacts showed when a participant with one child employed condoms or family planning service providers as the deciders of contraceptive methods introduced intrauterine devices, the occurrence of induced abortions was more likely to be reduced. Women who underwent side effects using pills were more likely to have had induced abortions. PMID:27783059

  12. Assessment of cultivation factors that affect biomass and geraniol production in transgenic tobacco cell suspension cultures.

    Directory of Open Access Journals (Sweden)

    Nikolay Vasilev

    Full Text Available A large-scale statistical experimental design was used to determine essential cultivation parameters that affect biomass accumulation and geraniol production in transgenic tobacco (Nicotiana tabacum cv. Samsun NN cell suspension cultures. The carbohydrate source played a major role in determining the geraniol yield and factors such as filling volume, inoculum size and light were less important. Sucrose, filling volume and inoculum size had a positive effect on geraniol yield by boosting growth of plant cell cultures whereas illumination of the cultures stimulated the geraniol biosynthesis. We also found that the carbohydrates sucrose and mannitol showed polarizing effects on biomass and geraniol accumulation. Factors such as shaking frequency, the presence of conditioned medium and solubilizers had minor influence on both plant cell growth and geraniol content. When cells were cultivated under the screened conditions for all the investigated factors, the cultures produced ∼ 5.2 mg/l geraniol after 12 days of cultivation in shaking flasks which is comparable to the yield obtained in microbial expression systems. Our data suggest that industrial experimental designs based on orthogonal arrays are suitable for the selection of initial cultivation parameters prior to the essential medium optimization steps. Such designs are particularly beneficial in the early optimization steps when many factors must be screened, increasing the statistical power of the experiments without increasing the demand on time and resources.

  13. Cowpea production as affected by dry spells in no-tillage and conventional crop systems

    Directory of Open Access Journals (Sweden)

    Rômulo Magno Oliveira de Freitas

    2013-12-01

    Full Text Available The objective of this study was to evaluate the effect of different periods of water shortage in no-tillage and conventional crop systems on cowpea yield components and grain yield in the Mossoró-RN region. For this, an experiment was conducted using two tillage systems (conventional and no-tillage subjected to periods of irrigation suspension (2; 6; 10; 14; 18 end 22 days, started at flowering (34 days after sowing. Plants were harvested 70 days after sowing, and the studied variables were: Pods length (CV, number of grains per pod (NGV, number of pods per plant (NPP, the hundred grains weight (PCG and grain yield (kg ha-1. The no-tillage system is more productive than the conventional under both irrigation and water stress treatments. The water stress length affected grain yield and all yield components studied in a negative way, except for the hundred grains weight. Among the systems studied, the no-tillage provides higher values for the yield components, except the hundred grains weight.

  14. Biotic and abiotic factors affect green ash volatile production and emerald ash borer adult feeding preference.

    Science.gov (United States)

    Chen, Yigen; Poland, Therese M

    2009-12-01

    The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is an exotic woodborer first detected in 2002 in Michigan and Ontario and is threatening the ash resource in North America. We examined the effects of light exposure and girdling on green ash (Fraxinus pennsylvanica Marsh) volatile production, and effects of light exposure, girdling, and leaf age on emerald ash borer adult feeding preferences and phototaxis. Green ash seedlings grown under higher light exposure had lower amounts of three individual volatile compounds, (Z)-3-hexenol, (E)-beta-ocimene, and (Z,E)-alpha-farnesene, as well as the total amount of six detected volatile compounds. Girdling did not affect the levels of these volatiles. Emerald ash borer females preferred mature leaves, leaves from girdled trees, and leaves grown in the sun over young leaves, leaves from nongirdled trees, and leaves grown in the shade, respectively. These emerald ash borer preferences were most likely because of physical, nutritional, or biochemical changes in leaves in response to the different treatments. Emerald ash borer females and males showed positive phototaxis in laboratory arenas, a response consistent with emerald ash borer preference for host trees growing in sunlight.

  15. Reproductive epidemiology

    DEFF Research Database (Denmark)

    Olsen, Jørn; Nøhr, Ellen Aagaard

    2010-01-01

    Reproductive health covers a broad category of health and disease conditions, according to the Cairo Statement. This chapter focuses on subfecundity fertility, fetal death, malformations, pregnancy complications, sexual health, and diseases that may have their origin in fetal life, but which will...

  16. Analysis on Factors Affecting of Reproductive Performance in Crossbred Pigs%杂交猪繁殖性能的影响因素分析

    Institute of Scientific and Technical Information of China (English)

    张省林; 白俊艳; 庞有志; 李刚; 李帅祥

    2011-01-01

    In this study, analysis of variance models of SPSS software were used to analyze the effect of different factors such as year, season, parity, birth, and cross mode on crossbred pigs reproductive performance. The following results showed that year gave a significant effect on numbers of piglets born alive and numbers of piglets at weaning (P0. 05). Season gave a significant effect on numbers of piglets born alive and numbers of piglets at weaning(P0. 05).%本研究利用SPSS软件中的方差分析等模块分析了年份、季节、胎次、性别和杂交方式等因素对杂交猪繁殖性能的影响.结果表明,年份对产活仔数和断奶仔猪数有极显著影响(P<0.01),而对总产仔数无显著影响(P>0.05).季节对产活仔数和断奶仔猪数有极显著影响(P<0.01),对总产仔数有显著影响(P<0.05).胎次对断奶仔猪数和初生重有极显著影响(P<0.01),对产活仔数和出生窝重有显著影响(P<0.05).公猪的初生重显著大于母猪的初生重(P<0.05).杂交方式对总产仔数、产活仔数、断奶仔猪数、出生窝重和初生重均无显著影响(P>0.05).

  17. EFFECT OF BYPASS FAT IN PRODUCTIVE AND REPRODUCTIVE PARAMETERS OF FEMALE CATTLE CROSSES INDICA SYNCHRONIZED WITH A CRESTAR® MODIFIED PROTOCOL - GUASDUALITO– VENEZUELA

    Directory of Open Access Journals (Sweden)

    Quintero AD

    2015-12-01

    Full Text Available The objetive of this study was to evaluate the effect of bypass fat in productive and reproductive parameters in bovine females indicates crosses in the Fundación livestock farm; of the parish Guasdualito Venezuela, Apure state, making a modification to Crestar® protocol. The fat by pass was provided by 52 days, to cows with an average of 3 was provided7 years of age, with no health problems and body condition from 2.75 to 3.5 (scale 1-5. The animals were inseminated into two groups and found that the average final body condition in the Gyr and crossbred cows improved; there is a positive correlation strong between the intensity and duration of heat and that the presence of the calves contributes to find animals with jealousy of short duration and low intensity.

  18. Reproductive endocrinology of vitamin D

    DEFF Research Database (Denmark)

    Lorenzen, Mette; Boisen, Ida Marie; Mortensen, Li Juel

    2017-01-01

    Vitamin D is a versatile hormone with several functions beyond its well-established role in maintenance of skeletal health and calcium homeostasis. The effects of vitamin D are mediated by the vitamin D receptor, which is expressed together with the vitamin D metabolizing enzymes...... in the reproductive tissues. The reproductive organs are therefore responsive to and able to metabolize vitamin D locally. The exact role remains to be clarified but several studies have suggested a link between vitamin D and production/release of reproductive hormones into circulation, which will be the main focus...... suffering from reproductive problems and abnormal endocrinology research addressing the role of vitamin D in reproductive endocrinology is of clinical importance....

  19. Effect of dietary energy source on energy balance, production, metabolic disorders and reproduction in lactating dairy cattle

    NARCIS (Netherlands)

    Knegsel, van A.T.M.; Brand, van den H.; Dijkstra, J.; Tamminga, S.; Kemp, B.

    2005-01-01

    The pathway for oxidation of energy involves a balanced oxidation of C2 and C3 compounds. During early lactation in dairy cattle this C2/C3 ratio is out of balance, due to a high availability of lipogenic (C2) products and a low availability of glycogenic (C3) products relative of the C2 and C3 prod

  20. Population dynamics of dechlorinators and factors affecting the level and products of PCB dechlorination in sediments

    Energy Technology Data Exchange (ETDEWEB)

    Kim, J.S.; Sokol, R.C.; Liu, X.; Bethoney, C.M.; Rhee, G.Y. [State Univ. of New York and New York State Department of Health, Albany, NY (United States)

    1996-12-31

    Microbial dechlorination of polychlorinated biphenyls (PCBs) often stops although a significant number of removable chlorines remain. To determine the reason for the cessation, we investigated the limitation of organic carbon, PCB bioavailability, and inhibition by metabolic products. Enrichment with carbon sources did not induce additional chlorination, indicating the plateau was not due to depletion of organic carbon. The bioavailability was not limiting, since a subcritical micelle concentration of the surfactant, which enhanced desorption without inhibiting dechlorinating microorganisms, failed to lower the plateau. Neither was it due to accumulation of metabolites, since no additional dechlorination was detected when plateau sediments were incubated with fresh medium. Similarly, dechlorination was not inhibited in freshly spiked sediment slurries. Dechlorination ended up at the same level with nearly identical congener profiles, regardless of treatment. These results indicate that cessation of dechlorination was due to the accumulation of daughter congeners, which cannot be used as electron acceptors by microbes. To determine whether the decreasing availability affected the microorganisms, we determined the population dynamics of dechlorinators using the most probable number technique. The growth dynamics of the dechlorinators mirrored the time course of dechlorination. It started when the population increased by two orders of magnitude. Once dechlorination stopped the dechlorinating population also began to decrease. When dechlorinators were inoculated into PCB-free sediments, the population decreased over time. The decrease of the population as dechlorination ceased confirms that the diminishing availability of congeners was the reason for the incomplete dechlorination. Recent findings have shown that a second phase of dechlorination of certain congeners can occur after a long lag. 45 refs., 8 figs.

  1. Bone Formation is Affected by Matrix Advanced Glycation End Products (AGEs) In Vivo.

    Science.gov (United States)

    Yang, Xiao; Mostafa, Ahmed Jenan; Appleford, Mark; Sun, Lian-Wen; Wang, Xiaodu

    2016-10-01

    Advanced glycation end products (AGEs) accumulate in bone extracellular matrix as people age. Although previous evidence shows that the accumulation of AGEs in bone matrix may impose significant effects on bone cells, the effect of matrix AGEs on bone formation in vivo is still poorly understood. To address this issue, this study used a unique rat model with autograft implant to investigate the in vivo response of bone formation to matrix AGEs. Fluorochrome biomarkers were sequentially injected into rats to label the dynamic bone formation in the presence of elevated levels of matrix AGEs. After sacrificing animals, dynamic histomorphometry was performed to determine mineral apposition rate (MAR), mineralized surface per bone surface (MS/BS), and bone formation rate (BFR). Finally, nanoindentation tests were performed to assess mechanical properties of newly formed bone tissues. The results showed that MAR, MS/BS, and BFR were significantly reduced in the vicinity of implant cores with high concentration of matrix AGEs, suggesting that bone formation activities by osteoblasts were suppressed in the presence of elevated matrix AGEs. In addition, MAR and BFR were found to be dependent on the surrounding environment of implant cores (i.e., cortical or trabecular tissues). Moreover, MS/BS and BFR were also dependent on how far the implant cores were away from the growth plate. These observations suggest that the effect of matrix AGEs on bone formation is dependent on the biological milieu around the implants. Finally, nanoindentation test results indicated that the indentation modulus and hardness of newly formed bone tissues were not affected by the presence of elevated matrix AGEs. In summary, high concentration of matrix AGEs may slow down the bone formation process in vivo, while imposing little effects on bone mineralization.

  2. Juvenile compensatory growth has negative consequences for reproduction in Trinidadian guppies (Poecilia reticulata).

    Science.gov (United States)

    Auer, Sonya K; Arendt, Jeffrey D; Chandramouli, Radhika; Reznick, David N

    2010-08-01

    Compensatory or 'catch-up' growth may be an adaptive mechanism that buffers the growth trajectory of young organisms from deviations caused by reduced food availability. Theory generally assumes that rapid juvenile compensatory growth impacts reproduction only through its positive effects on age and size at maturation, but potential reproductive costs to juvenile compensatory growth remain virtually unexplored. We used a food manipulation experiment to examine the reproductive consequences of compensatory growth in Trinidadian guppies (Poecilia reticulata). Compensatory growth did not affect adult growth rates, litter production rates or investment in offspring size. However, compensatory growth had negative effects on litter size, independent of the effects of female body length, resulting in a 20% decline in offspring production. We discuss potential mechanisms behind this observed cost to reproduction.

  3. 生育文化与社会再生产--以新疆阿克苏、喀什地区为例∗%Reproductive Culture and Social Re-production- Aksu and Kashgar as Examples

    Institute of Scientific and Technical Information of China (English)

    高卉; 袁年兴

    2013-01-01

      生育文化是基于人们的生育行为和实践所形成的一套意义体系,涵盖了人口再生产与文化再生产的重要议题,更是考察社会再生产机制的切入口之一。文章从生育知识、生育目的以及生育性别偏好等方面入手,分析认为新疆阿克苏、喀什地区的生育文化是以宗教为基础的、有别于现代医学观念的一套观念体系。旨在说明在现代化过程中,当地生育文化的变迁对社会再生产机制产生的内在影响,同时也为我国的少数民族政策的制定提供一定的借鉴。%The reproductive culture is a system based on birth behavior, covering the important issues of population reproduction and culture reproduction. It is also one of the starting points to observing the social reproduction. This paper, in terms of birth knowledge, birth purpose and the sex preference of births, analyzed and held that the reproductive culture in Kashgar and Aksu is religiously based, different from the concept system of modern medicine. It aims to illustrate the change of the local reproductive culture produces an impact on the social reproduction mechanism in the modernization process. It also aims to offer a reference in designing the ethnic minority policy in China.

  4. Biological control of soilborne diseases in organic potato production as affected by varying environmental conditions

    Science.gov (United States)

    Soilborne diseases are persistent problems in potato production and alternative management practices are needed, particularly in organic production, where control options are limited. Selected biocontrol organisms, including two naturally-occurring hypovirulent strains of Rhizoctonia solani (Rhs1a1 ...

  5. To grow or to seed: ecotypic variation in reproductive allocation and cone production by young female Aleppo pine (Pinus halepensis, Pinaceae).

    Science.gov (United States)

    Climent, José; Prada, M Aránzazu; Calama, Rafael; Chambel, M Regina; de Ron, David Sánchez; Alía, Ricardo

    2008-07-01

    Age and size at the first reproduction and the reproductive allocation of plants are linked to different life history strategies. Aleppo pine only reproduces through seed, and, as such, early female reproduction confers high fitness in its infertile highly fire-prone habitats along the Mediterranean coast because life expectancy is short. We investigated the extent of ecotypic differentiation in female reproductive allocation and examined the relation between early female reproduction and vegetative growth. In a common-garden experiment, the threshold age and size at first female reproduction and female reproductive allocation at age seven differed significantly among Aleppo pine provenances of ecologically distinct origin. Significant correlations among reproductive features of the provenances and the ecological traits of origin were found using different analytical tools. In nonlinear models of cone counts vs. stem volume, medium-sized trees (not the largest trees) produced the highest cone yield, confirming that, at the individual level, early female reproduction is incompatible with fast vegetative growth. The contribution of founder effects and adaptation to contrasting fire regimes may be confounding factors. But considering all traits analyzed, the geographical patterns of resource allocation by Aleppo pine suggest ecotypic specialization for either resource-poor (favoring early reproduction) or resource-rich (favoring vegetative growth) habitats.

  6. Situated embodied cognition: monitoring orientation cues affects product evaluation and choice

    NARCIS (Netherlands)

    J. Eelen; S. Dewitte; L. Warlop

    2013-01-01

    Consumers generally prefer products that are easy to interact with. In three studies, we show that this preference arises from the fit between product orientation and monitored situational constraints. Flexible right-handers, who monitor situational constraints, recall product orientations better an

  7. In vivo models for male reproductive toxicology.

    Science.gov (United States)

    Tyl, Rochelle W

    2002-05-01

    In Vivo Models for Male Reproductive Toxicology (Rochelle W. Tyl, Research Triangle Institute, Research Triangle Park, North Carolina). Assessment of male reproductive function requires a specific set of evaluations of the various steps in successful mating from sperm production to copulation to fertilization to production of a viable litter. This unit outlines the measurements that are standard for determining the effects of treatment with toxicant on the reproductive capacity of male mice and rats.

  8. The effects of oxidative stress on female reproduction: a review

    Directory of Open Access Journals (Sweden)

    Agarwal Ashok

    2012-06-01

    Full Text Available Abstract Oxidative stress (OS, a state characterized by an imbalance between pro-oxidant molecules including reactive oxygen and nitrogen species, and antioxidant defenses, has been identified to play a key role in the pathogenesis of subfertility in both males and females. The adverse effects of OS on sperm quality and functions have been well documented. In females, on the other hand, the impact of OS on oocytes and reproductive functions remains unclear. This imbalance between pro-oxidants and antioxidants can lead to a number of reproductive diseases such as endometriosis, polycystic ovary syndrome (PCOS, and unexplained infertility. Pregnancy complications such as spontaneous abortion, recurrent pregnancy loss, and preeclampsia, can also develop in response to OS. Studies have shown that extremes of body weight and lifestyle factors such as cigarette smoking, alcohol use, and recreational drug use can promote excess free radical production, which could affect fertility. Exposures to environmental pollutants are of increasing concern, as they too have been found to trigger oxidative states, possibly contributing to female infertility. This article will review the currently available literature on the roles of reactive species and OS in both normal and abnormal reproductive physiological processes. Antioxidant supplementation may be effective in controlling the production of ROS and continues to be explored as a potential strategy to overcome reproductive disorders associated with infertility. However, investigations conducted to date have been through animal or in vitro studies, which have produced largely conflicting results. The impact of OS on assisted reproductive techniques (ART will be addressed, in addition to the possible benefits of antioxidant supplementation of ART culture media to increase the likelihood for ART success. Future randomized controlled clinical trials on humans are necessary to elucidate the precise mechanisms

  9. Associations between age at first calving, rearing average daily weight gain, herd milk yield and dairy herd production, reproduction, and profitability.

    Science.gov (United States)

    Krpálková, L; Cabrera, V E; Kvapilík, J; Burdych, J; Crump, P

    2014-10-01

    The objective of this study was to evaluate the associations of variable intensity in rearing dairy heifers on 33 commercial dairy herds, including 23,008 cows and 18,139 heifers, with age at first calving (AFC), average daily weight gain (ADG), and milk yield (MY) level on reproduction traits and profitability. Milk yield during the production period was analyzed relative to reproduction and economic parameters. Data were collected during a 1-yr period (2011). The farms were located in 12 regions in the Czech Republic. The results show that those herds with more intensive rearing periods had lower conception rates among heifers at first and overall services. The differences in those conception rates between the group with the greatest ADG (≥0.800 kg/d) and the group with the least ADG (≤0.699 kg/d) were approximately 10 percentage points in favor of the least ADG. All the evaluated reproduction traits differed between AFC groups. Conception at first and overall services (cows) was greatest in herds with AFC ≥800 d. The shortest days open (105 d) and calving interval (396 d) were found in the middle AFC group (799 to 750 d). The highest number of completed lactations (2.67) was observed in the group with latest AFC (≥800 d). The earliest AFC group (≤749 d) was characterized by the highest depreciation costs per cow at 8,275 Czech crowns (US$414), and the highest culling rate for cows of 41%. The most profitable rearing approach was reflected in the middle AFC (799 to 750 d) and middle ADG (0.799 to 0.700 kg) groups. The highest MY (≥8,500 kg) occurred with the earliest AFC of 780 d. Higher MY led to lower conception rates in cows, but the highest MY group also had the shortest days open (106 d) and a calving interval of 386 d. The same MY group had the highest cow depreciation costs, net profit, and profitability without subsidies of 2.67%. We conclude that achieving low AFC will not always be the most profitable approach, which will depend upon farm

  10. The effect of group size on the interplay between dominance and reproduction in Bombus terrestris.

    Science.gov (United States)

    Amsalem, Etya; Hefetz, Abraham

    2011-03-28

    Social insects provide good model systems for testing trade-offs in decision-making because of their marked reproductive skew and the dilemma workers face when to reproduce. Attaining reproductive skew requires energy investment in aggression or fertility signaling, creating a trade-off between reproduction and dominance. This may be density-dependent because the cost of achieving dominance may be higher in larger groups. We investigated the effect of group-size in B. terrestris queenless workers on two major reproduction-dominance correlates: between-worker aggression, and pheromone production, aiming at mimicking decision-making during the transition of worker behavior from cooperation and sterility to aggressive reproductive competition in whole colonies. Despite the competition, reproductive division of labor in colonies can be maintained even during this phase through the production of a sterility signal by sterile workers that has an appeasement effect on dominant nestmates. Worker-worker aggression, ovary activation, and production of sterility-appeasement signals may therefore constitute components of a trade-off affecting worker reproduction decisions. By constructing queenless groups of different size and measuring how this affected the parameters above, we found that in all groups aggression was not evenly distributed with the α-worker performing most of the aggressive acts. Moreover, aggression by the α-worker increased proportionally with group-size. However, while in small groups the α-worker monopolized reproduction, in larger groups several workers shared reproduction, creating two worker groups: reproductives and helpers. It appears that despite the increase of aggression, this was evidently not sufficient for the α-worker to monopolize reproduction. If we compare the α-worker to the queen in full-sized colonies it can be hypothesized that worker reproduction in B. terrestris colonies starts due to a gradual increase in the worker population

  11. Acute, chronic and reproductive effects of petroleum and two petroleum substitutes on the large milkweed bug, Oncopeltus fasciatus

    Energy Technology Data Exchange (ETDEWEB)

    Merchant, E.R.; Walton, B.T.

    1983-12-01

    Effects of petroleum and two synthetic oils on mortality and reproduction of the large milkweed bug, Oncopeltus fasciatus (Dallas), were compared. Reproduction was investigated because of its importance in the maintenance of population levels and the sensitivity of the reproductive system to toxicants. Adult milkweed bugs were dosed topically with the test oils to measure acute toxicity. Chronic toxicity was determined by mean survival after chronic exposure, starting with the fifth instar. The ability of sublethal levels of the test oils to affect reproduction was measured by the number of eggs laid and their percent hatch. The two synthetic oils were found to be more acutely and chronically toxic than petroleum. Egg production was not affected by petroleum but was reduced approximately 20% by sublethal levels of both synthetic oils. Egg viability was not affected.

  12. Terroir et vignoble: how the farming management can affect the production of a quality wine

    Science.gov (United States)

    Gallo, Alba; Bini, Claudio

    2016-04-01

    Italian wine is one of the most exported wine in the world. The particular climate, the soil characteristics and other several factors have contributed to this success. Italy is located in the temperate belt, with a suitable climate for grapevine cultivation. For this reason, all regions in Italy produce wine, first of all the Veneto region, with 8.569.000 hl of wine in 2011. Wine quality derives from the perfect interaction among climate, morphology, soil and plant, i.e. the terroir. So, knowledge of the land characteristics, together with cultivation techniques and management, is essential to understand this interaction and the typicality of the wine. For example, large utilization of fertilizers and pesticides may determine accumulation of toxic substances in soil and possible translocation to the food chain. For this reason, metal contamination of soils and plants becomes a main issue in agricultural production. Therefore, our attention was focused on the determination of soil quality of the Prosecco DOCG (controlled and guaranteed denomination of origin) area, particularly in Conegliano. Conegliano is a town located in Veneto, in the province of Treviso, known for its wine. This wine variety is regulated by the Conegliano-Valdobbiadene production Consortium, to protect both consumers and producers. The goals of this research are: evaluation of trace metal content (Al ,Cd, Cr, Cu, Fe, Mg, Mn, Ni, P, Pb, V and Zn) in soils and possible uptake by grape leaves; estimation of biological soil quality (QBS-ar index); analysis of oxidative stress in dandelion (Taraxacum officinale) and grape leaves, by the Lipid peroxidation test (LPO test). Results concerning trace metal concentration show: i) a high content of Al, Mg and P in soils, and ii) high concentration of Al, Cu, Fe and Zn in grape leaves. High contents of Al in topsoil are consistent with the high concentration of organic matter. Instead, high Al contents in subsoil are related to clay. Mg and P are usually

  13. Diet micronutrient balance matters: How the ratio of dietary sterols/steroids affects development, growth and reproduction in two lepidopteran insects.

    Science.gov (United States)

    Jing, Xiangfeng; Grebenok, Robert J; Behmer, Spencer T

    2014-08-01

    Insects lack the ability to synthesize sterols de novo so they acquire this essential nutrient from their food. Cholesterol is the dominant sterol found in most insects, but in plant vegetative tissue it makes up only a small fraction of the total sterol profile. Instead, plants mostly contain phytosterols; plant-feeding insects generate the majority of their cholesterol by metabolizing phytosterols. However, not all phytosterols are readily converted to cholesterol, and some are even deleterious when ingested above a threshold level. In a recent study we showed that caterpillars reared on tobacco accumulating novel sterols/steroids exhibited reduced performance, even when suitable sterols were present. In the current study we examined how the dominant sterols (cholesterol and stigmasterol) and steroids (cholestanol and cholestanone) typical of the modified tobacco plants affected two insect herbivores (Heliothis virescens and Helicoverpa zea). The sterols/steroids were incorporated into synthetic diets singly, as well as in various combinations, ratios and amounts. For each insect species, a range of performance values was recorded for two generations, with the eggs from the 1st-generation adults as the source of neonates for the 2nd-generation. Performance on the novel steroids (cholestanol and cholestanone) was extremely poor compared to suitable sterols (cholesterol and stigmasterol). Additionally, performance tended to decrease as the ratio of the novel dietary steroids increased. We discuss how the balance of different dietary sterols/steroids affected our two caterpillar species, relate this back to recent studies on sterol/steroid metabolism in these two species, and consider the potential application of sterol/steroid modification in crops.

  14. Intraspecific variation in body size and the rate of reproduction in female insects - adaptive allometry or biophysical constraint?

    Science.gov (United States)

    Berger, David; Olofsson, Martin; Friberg, Magne; Karlsson, Bengt; Wiklund, Christer; Gotthard, Karl; Gilburn, Andre

    2012-11-01

    1. A high rate of reproduction may be costly if ecological factors limit immediate reproductive output as a fast metabolism compromises own future survival. Individuals with more reserves need more time and opportunity to realize their reproductive potential. Theory therefore predicts that the reproductive rate, defined as the investment in early reproduction in proportion to total potential, should decrease with body size within species. 2. However, metabolic constraints on body size- and temperature-dependent biological rates may impede biophysical adaptation. Furthermore, the sequential manner resources that are allocated to somatic vs. reproductive tissue during ontogeny may, when juveniles develop in unpredictable environments, further contribute to non-adaptive variation in adult reproductive rates. 3. With a model on female egg laying in insects, we demonstrate how variation in body reserves is predicted to affect reproductive rate under different ecological scenarios. Small females always have higher reproductive rates but shorter lifespans. However, incorporation of female host selectivity leads to more similar reproductive rates among female size classes, and oviposition behaviour is predicted to co-evolve with reproductive rate, resulting in small females being more selective in their choice and gaining relatively more from it. 4. We fed simulations with data on the butterfly Pararge aegeria to compare model predictions with reproductive rates of wild butterflies. However, simulated reproductive allometry was a poor predictor of that observed. Instead, reproductive rates were better explained as a product of metabolic constraints on rates of egg maturation, and an empirically derived positive allometry between reproductive potential and size. However, fitness is insensitive to moderate deviations in reproductive rate when oviposition behaviour is allowed to co-evolve in the simulations, suggesting that behavioural compensation may mitigate putative

  15. A global analysis of fine root production as affected by soil nitrogen and phosphorus.

    Science.gov (United States)

    Yuan, Z Y; Chen, Han Y H

    2012-09-22

    Fine root production is the largest component of belowground production and plays substantial roles in the biogeochemical cycles of terrestrial ecosystems. The increasing availability of nitrogen (N) and phosphorus (P) due to human activities is expected to increase aboveground net primary production (ANNP), but the response of fine root production to N and P remains unclear. If roots respond to nutrients as ANNP, fine root production is anticipated to increase with increasing soil N and P. Here, by synthesizing data along the nutrient gradient from 410 natural habitats and from 469 N and/or P addition experiments, we showed that fine root production increased in terrestrial ecosystems with an average increase along the natural N gradient of up to 0.5 per cent with increasing soil N. Fine root production also increased with soil P in natural conditions, particularly at P soil types. The global average increases in fine root production are lower than those of ANNP, indicating that above- and belowground counterparts are coupled, but production allocation shifts more to aboveground with higher soil nutrients. Our results suggest that the increasing fertilizer use and combined N deposition at present and in the future will stimulate fine root production, together with ANPP, probably providing a significant influence on atmospheric CO(2) emissions.

  16. The use of HPLC in the control of Neem commercial products quality: reproduction of the insecticide action; Uso de CLAE no controle de qualidade em produtos comerciais de Nim: reprodutibilidade da acao inseticida

    Energy Technology Data Exchange (ETDEWEB)

    Forim, Moacir Rossi; Matos, Andreia Pereira; Silva, Maria Fatima das Gracas Fernandes da; Cass, Quezia Bezerra; Vieira, Paulo Cezar; Fernandes, Joao Batista, E-mail: mrforim@ufscar.b [Universidade Federal de Sao Carlos (DQ/UFSCar), SP (Brazil). Dept. de Quimica

    2010-07-01

    The Neem tree, Azadirachta indica, provides many useful compounds that are used as pesticides. However, the efficiency in field of products like neem oil can be committed because they have not been observed reproductive content of secondary metabolic like azadirachtin. Based on reverse-phase high-performance liquid chromatography (HPLC) a new method was developed to permit the rapid quantitative analysis of azadirachtin from seeds, extracts and oil of Neem. In the present study it was evaluated the azadirachtin quantitative variation among various Neem's extracts and seeds showing the importance of quality control for reproduction of the insecticide efficiency, using S. frugiperda as target insect. (author)

  17. Do recent US Supreme Court rulings on patenting of genes and genetic diagnostics affect the practice of genetic screening and diagnosis in prenatal and reproductive care?

    Science.gov (United States)

    Chandrasekharan, Subhashini; McGuire, Amy L; Van den Veyver, Ignatia B

    2014-10-01

    Thousands of patents have been awarded that claim human gene sequences and their uses, and some have been challenged in court. In a recent high-profile case, Association for Molecular Pathology, et al. v. Myriad Genetics, Inc., et al., the US Supreme Court ruled that genes are natural occurring substances and therefore not patentable through 'composition of matter' claims. The consequences of this ruling will extend well beyond ending Myriad's monopoly over BRCA testing and may affect similar monopolies of other commercial laboratories for tests involving other genes. It could also simplify intellectual property issues surrounding genome-wide clinical sequencing, which can generate results for genes covered by intellectual property. Non-invasive prenatal testing (NIPT) for common aneuploidies using cell-free fetal (cff) DNA in maternal blood is currently offered through commercial laboratories and is also the subject of ongoing patent litigation. The recent Supreme Court decision in the Myriad case has already been invoked by a lower district court in NIPT litigation and resulted in invalidation of primary claims in a patent on currently marketed cffDNA-based testing for chromosomal aneuploidies.

  18. Analyzing factors Affecting Consumers’ Attitude & Intention to Purchase Counterfeit products of luxury Brands In clothing Industry

    Directory of Open Access Journals (Sweden)

    Abdolhamid Ebrahimi

    2012-01-01

    Full Text Available One of the world trends growing alarmingly is Producing, distributing & Consuming counterfeit goods of Credible & luxury brands & one of the industries facing this crisis increasingly is clothing industry. Based on this, the present study aims to analyze & identify factors influencing Consumers’ attitude toward counterfeit products & intention to purchase such products in clothing industry that has been done in Sari, Mazandaran. And in it, these Factors effect on attitude toward counterfeit products & then the impact of attitude toward counterfeit products on intention to buy such products have been measured. The study method is a Survey type & in order to collect study data, a questionnaire designed with a combination of different available resources in literature has been used. To achieve the study goals, 384 individuals who purchased counterfeit products intentionally were chosen from Sari. To analyze data & test hypotheses, Study model & questionnaire, Cronbach's alpha coefficient tests, Structural Equations Modeling (SEM, Confirmatory Factorial Analysis (CFA & one-way Variance analysis (ANONA have been applied & two Software Packages SPSS & LISREL were used. Through reviewing the literature available & analyzing experts’ views, generally, 11 variables have been identified: Personal gratification, Value consciousness, Price–quality inference, Social Effect, Brand prestige, Brand Loyalty, Ethical Issues, Risk averseness, Subjective norm, perceived risk, Brand consciousness, as the influencing factors on attitude toward counterfeit products. Study Findings have indicated that the factors as Personal gratification, Value consciousness, Price–quality inference, Social Effect, Ethical Issues, Subjective norm, perceived risk, Brand consciousness, have meaningful effect on attitude to counterfeit products & the impact of Brand prestige, Brand Loyalty, Risk averseness, was not meaningfull on view about counterfeit products. Besides, the

  19. Analyzing factors Affecting Consumers’ Attitude & Intention to Purchase Counterfeit products of luxury Brands In clothing Industry

    Directory of Open Access Journals (Sweden)

    abdolhamid ebrahimi

    2012-12-01

    Full Text Available One of the world trends growing alarmingly is Producing, distributing & Consuming counterfeit goods of Credible & luxury brands & one of the industries facing this crisis increasingly is clothing industry. Based on this, the present study aims to analyze & identify factors influencing Consumers’ attitude toward counterfeit products & intention to purchase such products in clothing industry that has been done in Sari, Mazandaran. And in it, these Factors effect on attitude toward counterfeit products & then the impact of attitude toward counterfeit products on intention to buy such products have been measured. The study method is a Survey type & in order to collect study data, a questionnaire designed with a combination of different available resources in literature has been used. To achieve the study goals, 384 individuals who purchased counterfeit products intentionally were chosen from Sari. To analyze data & test hypotheses, Study model & questionnaire, Cronbach's alpha coefficient tests, Structural Equations Modeling (SEM, Confirmatory Factorial Analysis (CFA & one-way Variance analysis (ANONA have been applied & two Software Packages SPSS & LISREL were used. Through reviewing the literature available & analyzing experts’ views, generally, 11 variables have been identified: Personal gratification, Value consciousness, Price–quality inference, Social Effect, Brand prestige, Brand Loyalty, Ethical Issues, Risk averseness, Subjective norm, perceived risk, Brand consciousness, as the influencing factors on attitude toward counterfeit products. Study Findings have indicated that the factors as Personal gratification, Value consciousness, Price–quality inference, Social Effect, Ethical Issues, Subjective norm, perceived risk, Brand consciousness, have meaningful effect on attitude to counterfeit products & the impact of Brand prestige, Brand Loyalty, Risk averseness, was not meaningfull on view about counterfeit products. Besides, the

  20. Wolbachia Influences the Production of Octopamine and Affects Drosophila Male Aggression.

    Science.gov (United States)

    Rohrscheib, Chelsie E; Bondy, Elizabeth; Josh, Peter; Riegler, Markus; Eyles, Darryl; van Swinderen, Bruno; Weible, Michael W; Brownlie, Jeremy C

    2015-07-01

    Wolbachia bacteria are endosymbionts that infect approximately 40% of all insect species and are best known for their ability to manipulate host reproductive systems. Though the effect Wolbachia infection has on somatic tissues is less well understood, when present in cells of the adult Drosophila melanogaster brain, Wolbachia exerts an influence over behaviors related to olfaction. Here, we show that a strain of Wolbachia influences male aggression in flies, which is critically important in mate competition. A specific strain of Wolbachia was observed to reduce the initiation of aggressive encounters in Drosophila males compared to the behavior of their uninfected controls. To determine how Wolbachia was able to alter aggressive behavior, we investigated the role of octopamine, a neurotransmitter known to influence male aggressive behavior in many insect species. Transcriptional analysis of the octopamine biosynthesis pathway revealed that two essential genes, the tyrosine decarboxylase and tyramine β-hydroxylase genes, were significantly downregulated in Wolbachia-infected flies. Quantitative chemical analysis also showed that total octopamine levels were significantly reduced in the adult heads.

  1. Effect of yeast culture on milk production and metabolic and reproductive performance of early lactation dairy cows

    Directory of Open Access Journals (Sweden)

    Kalmus Piret

    2009-08-01

    Full Text Available Abstract Background The main objective of this study was to estimate the effect of supplementation with Saccaromyces cerevisiae (SC (Yea-Sacc® 1026 on milk production, metabolic parameters and the resumption of ovarian activity in early lactation dairy cows. Methods The experiment was conducted during 2005/2006 in a commercial tied-house farm with an average of 200 milking Estonian Holstein Friesian cows. The late pregnant multiparous cows (n = 46 were randomly divided into two groups; one group received 10 g yeast culture from two weeks before to 14 weeks after calving. The groups were fed a total mixed ration with silages and concentrates. Milk recording data and blood samples for plasma metabolites were taken. Resumption of luteal activity was determined using milk progesterone (P4 measurements. Uterine bacteriology and ovarian ultrasonography (US were performed and body condition scores (BCS and clinical disease occurrences were recorded. For analysis, the statistical software Stata 9.2 and R were used to compute Cox proportional hazard and linear mixed models. Results The average milk production per cow did not differ between the groups (32.7 ± 6.4 vs 30.7 ± 5.3 kg/day in the SC and control groups respectively, but the production of milk fat (P P 4 results, all cows in both groups ovulated during the experimental period. The resumption of ovarian activity (first ovulations and time required for elimination of bacteria from the uterus did not differ between the groups. Conclusion Supplementation with SC had an effect on milk protein and fat production, but did not influence the milk yield. No effects on PP metabolic status, bacterial elimination from the uterus nor the resumption of ovarian activity were found.

  2. Effect of yeast culture on milk production and metabolic and reproductive performance of early lactation dairy cows

    OpenAIRE

    Kalmus Piret; Orro Toomas; Waldmann Andres; Lindjärv Raivo; Kask Kalle

    2009-01-01

    Abstract Background The main objective of this study was to estimate the effect of supplementation with Saccaromyces cerevisiae (SC) (Yea-Sacc® 1026) on milk production, metabolic parameters and the resumption of ovarian activity in early lactation dairy cows. Methods The experiment was conducted during 2005/2006 in a commercial tied-house farm with an average of 200 milking Estonian Holstein Friesian cows. The late pregnant multiparous cows (n = 46) were randomly divided into two groups; one...

  3. They Raise Them Differently Up North - Different Production Practices in Australian Growing Areas May Affect Mycorrhizae

    Science.gov (United States)

    This report briefly summarizes the results from a survey to determine cultural blueberry practices and production differences used in New South Wales (NSW), Victoria, Tasmania, and Queensland. The purpose of the survey was to collect information on a number of production practices that had been s...

  4. 29 CFR 784.138 - Perishable state of the aquatic product as affecting exemption.

    Science.gov (United States)

    2010-07-01

    ... such nonperishable products as fish oil and fish meal, or canned seafood, are not within the exemption... or manufacturing operations on products previously rendered nonperishable, such as refining fish oil or handling fish meal in connection with the manufacture of feeds. Further specific examples...

  5. The effect of foeniculum vulgare essence on production performance, egg quality and reproductive parameters of laying hens

    Directory of Open Access Journals (Sweden)

    Abolghasem Taki

    2014-10-01

    Full Text Available An experiment was conducted to evaluate the effect of fennel essence on performance, egg quality and ovarian morphology of commercial laying eggs for ten weeks. In this experiment, one hundred and forty 30-wk-old commercial egg laying type Hy-Line (W36 were randomly assigned into 4 treatments with 5 replicates and 8 birds per each. Treatments were a control (0.0% essence and diets containing 200, 400 and 600 ppm fennel essence. Performance parameters include egg weight and egg production was recorded daily and feed intake, FCR and egg quality parameters were measured weekly. At the end of the experiment, 2 birds of each replicate was randomly selected and slaughtered for analysis of ovarian morphology. Different levels of fennel essence no significant effect on egg production. Different levels of fennel essence no significant effect on egg production. Levels of 200 and 400 ppm of fennel essence showed a significant increase in egg weight. Levels of fennel essence had no significant effect on feed intake and FCR but it caused significant increase in yolk color and had significant effect on the properties of egg shell. Levels of essence had no significant effect on the relative weight of the ovary, oviduct and stroma but 200 ppm of fennel essence had significant effect on weight of large yellow follicles. The results showed that the addition of fennel essence improved egg weight, egg shell and yolk color and had a significant effect on the large yellow follicles.

  6. The prediction of the methane production in landfill affected by the temperature

    Directory of Open Access Journals (Sweden)

    Zhou Lianying

    2015-01-01

    Full Text Available The functional relationship between the generation rate coefficient and temperature was developed for quantitative prediction of the temperature effect in this paper. The methane production of the landfill was predicted under the condition of the seasonal variation. The results showed that considering the temperature effect, the methane production is higher than the methane production without temperature effect of the 0.14×106 m3~0.28×106m3.With the depth increasing, the effect of the atmospheric temperature fluctuation on the temperature change of the landfill was weakened. The temperature has a significant effect on the methane production in the landfill. The temperature effect should be considered when simulate the long time effect of the landfill methane production.

  7. Does species richness affect fine root biomass and production in young forest plantations?

    DEFF Research Database (Denmark)

    Domisch, Timo; Finér, Leena; Dawud, Seid Muhie;

    2015-01-01

    species composition from fine root biomass samples with the near-infrared reflectance spectroscopy method. We did not observe higher biomass or production in mixed stands compared to monocultures. Neither did we observe any differences in tree root length or fine root turnover. One reason for this could......Tree species diversity has been reported to increase forest ecosystem above-ground biomass and productivity, but little is known about below-ground biomass and production in diverse mixed forests compared to single-species forests. For testing whether species richness increases below-ground biomass...... and production and thus complementarity between forest tree species in young stands, we determined fine root biomass and production of trees and ground vegetation in two experimental plantations representing gradients in tree species richness. Additionally, we measured tree fine root length and determined...

  8. Distribution and abundance of predators that affect duck production--prairie pothole region

    Science.gov (United States)

    Sargeant, A.B.; Greenwood, R.J.; Sovada, M.A.; Shaffer, T.L.

    1993-01-01

    During 1983-88, the relative abundance of 18 species and species-groups of mammalian and avian predators affecting duck production in the prairie pothole region was determined in 33 widely scattered study areas ranging in size from 23-26 km2. Accounts of each studied species and species-group include habitat and history, population structure and reported densities, and information on distribution and abundance from the present study. Index values of undetected, scarce, uncommon, common, or numerous were used to rate abundance of nearly all species in each study area. Principal survey methods were livetrapping of striped skunks (Mephitis mephitis) and Franklin's ground squirrels (Spermophilus franklinii), systematic searches for carnivore tracks in quarter sections (0.65 km2), daily records of sightings of individual predator species, and systematic searches for occupied nests of tree-nesting avian predators. Abundances of predators in individual areas were studied 1-3 years.The distribution and abundance of predator species throughout the prairie pothole region have undergone continual change since settlement of the region by Europeans in the late 1800's. Predator populations in areas we studied differed markedly from those of pristine times. The changes occurred from habitat alterations, human-inflicted mortality of predators, and interspecific relations among predator species. Indices from surveys of tracks revealed a decline in the abundance of red foxes (Vulpes vulpes) and an albeit less consistent decline in the abundance of raccoons (Procyon lotor) with an increase in the abundance of coyotes (Canis latrans). Records of locations of occupied nests revealed great horned owls (Bubo virginianus) and red-tailed hawks (Buteo jamaicensis) tended to nest 0.5 km apart, and American crows (Corvus brachyrhynchos) tended to avoid nesting 0.5 km of nests of red-tailed hawks. Excluding large gulls, for which no measurements of abundance were obtained, the number of

  9. How can farming intensification affect the environmental impact of milk production?

    Science.gov (United States)

    Bava, L; Sandrucci, A; Zucali, M; Guerci, M; Tamburini, A

    2014-07-01

    The intensification process of the livestock sector has been characterized in recent decades by increasing output of product per hectare, increasing stocking rate, including more concentrated feed in the diet, and improving the genetic merit of the breeds. In dairy farming, the effects of intensification on the environmental impact of milk production are not completely clarified. The aim of the current study was to assess the environmental impacts of dairy production by a life cycle approach and to identify relations between farming intensity and environmental performances expressed on milk and land units. A group of 28 dairy farms located in northern Italy was involved in the study; data collected during personal interviews of farmers were analyzed to estimate emissions (global warming potential, acidification, and eutrophication potentials) and nonrenewable source consumption (energy and land use). The environmental impacts of milk production obtained from the life cycle assessment were similar to those of other recent studies and showed high variability among the farms. From a cluster analysis, 3 groups of farms were identified, characterized by different levels of production intensity. Clusters of farms showed similar environmental performances on product basis, despite important differences in terms of intensification level, management, and structural characteristics. Our study pointed out that, from a product perspective, the most environmentally friendly way to produce milk is not clearly identifiable. However, the principal component analysis showed that some characteristics related to farming intensification, such as milk production per cow, dairy efficiency, and stocking density, were negatively related to the impacts per kilogram of product, suggesting a role of these factors in the mitigation strategy of environmental burden of milk production on a global scale. Considering the environmental burden on a local perspective, the impacts per hectare were

  10. Female Reproductive System

    Science.gov (United States)

    ... Reproductive System en español Sistema reproductor femenino About Human Reproduction All living things reproduce. Reproduction — the process by ... male and female reproductive systems are essential for reproduction. The female needs a ... like other organisms, pass certain characteristics of themselves ...

  11. Hypoxaemia affects male reproduction: a case study of how to differentiate between primary and secondary hypoxic testicular toxicity due to chemical exposure.

    Science.gov (United States)

    Bomhard, Ernst M; Gelbke, Heinz-Peter

    2013-07-01

    Classification for fertility is based on two conditions, namely on evidence of an adverse effect on sexual function and fertility and that the effect is not secondary to other toxic effects. To decide on an adverse effect is a relatively simple day-to-day decision in toxicology but whether this effect is secondary often leads to serious controversy. As the seminiferous epithelium operates on the verge of hypoxia, oxygen deficit can lead to secondary impairment of testicular function. This is well known from healthy mountaineers exposing themselves to high altitude. They have reduced blood oxygen content that goes in parallel with impairment of testicular function and this effect remains for some time in spite of a compensatory polycythaemia. Similar findings are described for experimental animals exposed to hypobaric oxygen/high altitude. In addition, testicular function is affected in severe diseases in humans associated with systemic oxygen deficit like chronic obstructive pulmonary disease, sickle cell disease or beta-thalassaemia as well as in transgenic animals simulating haemolytic anaemia or sickle cell disease. The problem of insufficient oxygen supply as the underlying cause for testicular impairment has received relatively little attention in toxicology, mainly because blood oxygen content is generally not measured in these animal experiments. The difficulties associated with the decision whether testicular toxicity is primary or secondary to hypoxia are exemplified by the results of inhalation studies with nickel subsulphide and gallium arsenide (GaAs). Both of these particulate substances lead to severe lung toxicity that might impair oxygen uptake, but testicular toxicity is only observed with GaAs. This may first be explained by different effects on the blood: nickel subsulphide inhalation leads to a compensatory erythropoiesis that may mitigate pulmonary lack of oxygen uptake. In contrast, GaAs exposure is associated with microcytic haemolytic

  12. Male fertility versus sterility, cytotype, and DNA quantitative variation in seed production in diploid and tetraploid sea lavenders (Limonium sp., Plumbaginaceae) reveal diversity in reproduction modes.

    Science.gov (United States)

    Róis, Ana Sofia; Teixeira, Generosa; Sharbel, Timothy F; Fuchs, Jörg; Martins, Sérgio; Espírito-Santo, Dalila; Caperta, Ana D

    2012-12-01

    The genus Limonium Miller, a complex taxonomic group, comprises annuals and perennials that can produce sexual and/or asexual seeds (apomixis). In this study, we used diverse cytogenetic and cytometric approaches to analyze male sporogenesis and gametogenesis for characterizing male reproductive output on seed production in Limonium ovalifolium and Limonium multiflorum. We showed here that the first species is mostly composed of diploid cytotypes with 2n = 16 chromosomes and the latter species by tetraploid cytotypes with 2n = 32, 34, 35, 36 chromosomes and had a genome roughly twice as big as the former one. In both species, euploid and aneuploid cytotypes with large metacentric chromosomes having decondensed interstitial sites were found within and among populations, possibly involved in chromosomal reconstructions. L. ovalifolium diploids showed regular meiosis resulting in normal tetrads, while diverse chromosome pairing and segregation irregularities leading to the formation of abnormal meiotic products are found in balanced and non-balanced L. multiflorum tetraploids. Before anther dehiscence, the characteristic unicellular, bicellular, or tricellular pollen grains showing the typical Limonium micro- or macro-reticulate exine ornamentation patterns were observed in L. ovalifolium using scanning electron microscopy. Most of these grains were viable and able to produce pollen tubes in vitro. In both balanced and unbalanced L. multiflorum tetraploids, microspores only developed until the "ring-vacuolate stage" with a collapsed morphology without the typical exine patterns, pointing to a sporophytic defect. These microspores were unviable and therefore never germinated in vitro. L. ovalifolium individuals presented larger pollen grains than those of L. multiflorum, indicating that pollen size and ploidy levels are not correlated in the Limonium system. Cytohistological studies in mature seeds from both species revealed that an embryo and a residual endosperm

  13. How body mass and lifestyle affect juvenile biomass production in placental mammals.

    Science.gov (United States)

    Sibly, Richard M; Grady, John M; Venditti, Chris; Brown, James H

    2014-02-22

    In mammals, the mass-specific rate of biomass production during gestation and lactation, here called maternal productivity, has been shown to vary with body size and lifestyle. Metabolic theory predicts that post-weaning growth of offspring, here termed juvenile productivity, should be higher than maternal productivity, and juveniles of smaller species should be more productive than those of larger species. Furthermore because juveniles generally have similar lifestyles to their mothers, across species juvenile and maternal productivities should be correlated. We evaluated these predictions with data from 270 species of placental mammals in 14 taxonomic/lifestyle groups. All three predictions were supported. Lagomorphs, perissodactyls and artiodactyls were very productive both as juveniles and as mothers as expected from the abundance and reliability of their foods. Primates and bats were unproductive as juveniles and as mothers, as expected as an indirect consequence of their low predation risk and consequent low mortality. Our results point the way to a mechanistic explanation for the suite of correlated life-history traits that has been called the slow-fast continuum.

  14. Ovulation but not milt production is inhibited in fathead minnows (Pimephales promelas) exposed to a reproductively inhibitory pulp mill effluent

    OpenAIRE

    Waye, Andrew; Lado, Wudu E; Martel, Pierre H; Arnason, John T.; Vance L Trudeau

    2014-01-01

    Background A 5-day fathead minnow (FHM) spawning assay is used by industry to monitor pulp mill effluent quality, with some mill effluents capable of completely inhibiting spawning. The purpose of this report is to characterize the effect of an inhibitory effluent on egg and milt production in FHM. Methods Eight tanks were treated with an inhibitory effluent while eight were kept with clean water. Each tank contained two males and four females as per the 5-day FHM spawning assay used by indus...

  15. Compost amendment of sandy soil affects soil properties and greenhouse tomato productivity

    DEFF Research Database (Denmark)

    Arthur, Emmanuel; Cornelis, W.; Razzaghi, Fatemeh

    2012-01-01

    Sandy soils, with low productivity, could be improved by compost application to sustain crop production. This study aimed to examine the effect of three compost types (vegetable, fruit and yard waste compost, garden waste compost, and spent mushroom compost) on basic properties of a loamy sand...... and greenhouse tomato productivity. Disturbed and intact soil samples were taken from a decade-long compost field experiment on loamy sand with three compost types at application rate of 30 m3 ha-1 yr-1 (7.5 ton ha-1 yr-1). The soils were characterized for chemical and physical properties. Tomato was planted...... in a greenhouse using soil samples from the field and vegetative and yield parameters (plant height, stem diameter, leaf number, and fruit yield), water productivity, and harvest index were evaluated. All compost types significantly increased soil total carbon, total nitrogen, pH, electrical conductivity...

  16. Biogas production as affected by heavy metals in the anaerobic digestion of sludge

    Directory of Open Access Journals (Sweden)

    Hussein I. Abdel-Shafy

    2014-12-01

    The sewage sludge samples were separated from the sewage water of the pilot plant at the National Research Centre, TDC site. The effect of heavy metals on the biogas production of the anaerobic digester was studied. The inhibitory effect on the biogas production and toxic level of metals was determined in this study. The general ranking of heavy metal toxicity appears to be Hg > Cd > Cr (III. The present investigation reveals that heavy metals in addition to the anaerobic digester decreased the biogas production as an indication of efficiency of the process. A significant decrease in gas production and volatile organic matter removal was obtained. It was also noted that an accumulation of organic acid intermediates was obtained as a result of methanogenic bacterial inhibition. This accumulation was limited during the pulse feed of metals. This is due to the rapid poisoning of the active bacterial forms in the digester.

  17. Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint

    Directory of Open Access Journals (Sweden)

    Cunningham Drew S

    2009-05-01

    Full Text Available Abstract Background Plasmids are being reconsidered as viable vector alternatives to viruses for gene therapies and vaccines because they are safer, non-toxic, and simpler to produce. Accordingly, there has been renewed interest in the production of plasmid DNA itself as the therapeutic end-product of a bioprocess. Improvement to the best current yields and productivities of such emerging processes would help ensure economic feasibility on the industrial scale. Our goal, therefore, was to develop a stoichiometric model of Escherichia coli metabolism in order to (1 determine its maximum theoretical plasmid-producing capacity, and to (2 identify factors that significantly impact plasmid production. Results Such a model was developed for the production of a high copy plasmid under conditions of batch aerobic growth on glucose minimal medium. The objective of the model was to maximize plasmid production. By employing certain constraints and examining the resulting flux distributions, several factors were determined that significantly impact plasmid yield. Acetate production and constitutive expression of the plasmid's antibiotic resistance marker exert negative effects, while low pyruvate kinase (Pyk flux and the generation of NADPH by transhydrogenase activity offer positive effects. The highest theoretical yield (592 mg/g resulted under conditions of no marker or acetate production, nil Pyk flux, and the maximum allowable transhydrogenase activity. For comparison, when these four fluxes were constrained to wild-type values, yields on the order of tens of mg/g resulted, which are on par with the best experimental yields reported to date. Conclusion These results suggest that specific plasmid yields can theoretically reach 12 times their current experimental maximum (51 mg/g. Moreover, they imply that abolishing Pyk activity and/or transhydrogenase up-regulation would be useful strategies to implement when designing host strains for plasmid

  18. Characteristics affecting management of design information in the production system design process

    OpenAIRE

    Bruch, Jessica; Bellgran, Monica

    2013-01-01

    Although it has been argued that the design of production systems is crucial, there is a general lack of empirical studies analysing and identifying resources and capabilities required for an efficient production system design process. One of these resources is the critical role attributed to design information and one such capability is how the design information is managed. To address this research gap, this paper reports the results from two in-depth case studies in the automotive industry...

  19. Long-term reproductive behaviour of woody plants across seven Bornean forest types in the Gunung Palung National Park (Indonesia): suprannual synchrony, temporal productivity and fruiting diversity.

    Science.gov (United States)

    Cannon, Charles H; Curran, Lisa M; Marshall, Andrew J; Leighton, Mark

    2007-10-01

    For 68 months, we observed the reproductive behaviour of 7288 woody plants (172 figs, 1457 climbers and 5659 trees) spanning major soil and elevational gradients. Two 2-3 month community-wide supra-annual fruiting events were synchronized across five forest types, coinciding with ENSO events. At least 27 genera in 24 families restricted their reproduction to these events, which involved a substantial proportion of tree diversity (> 80% of phylogenetic diversity). During these events, mean reproductive levels (8.5%) represented an almost four-fold increase compared with other months. These patterns indicate a strong behavioural advantage to this unusual reproductive behaviour. Montane forest experienced a single, separate fruiting peak while the peat swamp forest did not participate. Excluding these events, no temporal reproductive pattern was detectable, at either the landscape or forest type. These phenological patterns have major implications for the conservation of frugivore communities, with montane and swamp forests acting as 'keystone' forests.

  20. Earthworm functional traits and interspecific interactions affect plant nitrogen acquisition and primary production

    NARCIS (Netherlands)

    Andriuzzi, Walter; Schmidt, Olaf; Brussaard, L.; Faber, J.H.; Bolger, T.

    2016-01-01

    We performed a greenhouse experiment to test how the functional diversity of earthworms, the dominant group of soil macro-invertebrates in many terrestrial ecosystems, affects nitrogen cycling and plant growth. Three species were chosen to represent a range of functional traits: Lumbricus terrestris

  1. DID THE 2004 CAP REFORM AFFECT PRODUCTION PRACTICES OF CEREALS? INSIGHTS FROM THE AGRICULTURAL INPUT SUPPLIERS

    Directory of Open Access Journals (Sweden)

    Theodoros MARKOPOULOS

    2015-10-01

    Full Text Available The Mid-term review of the Common Agricultural Policy in 2003/2004 has strengthened the multifunctional role of agriculture by implementing “decoupling”, “modulation” and “cross-compliance” and created a number of significant changes in agricultural production in all EU member states. Specifically, the reform shifted emphasis away from commodity support towards environmental contracts, diversified production practices and rural development. In the case of cereals, a full decoupling was applied in subsidies and integration through rights in the Single Payment Scheme, except rice, which was one of the few crop cases in which part of the subsidy remained coupled, particular in countries with significant production like Greece. Within this context, the present study aims to analyze the impact that the reformed CAP measures had on agricultural production and more specifically variations in production diversification. The novelty of this study is that instead of focusing on the producers, it targeted the agricultural input stores, so as to get better insights of the CAP reform impacts on a larger scale of the regional economy. Accordingly, primary data were collected through personal interviews (structured questionnaire from 209 owners of agricultural input stores in the region of Anatoliki Makedonia and Thraki and were analyzed through multivariate data analysis. The results identify important antecedents for the regional economy and the viability of agricultural input stores, which include factors of the reformed CAP, environmental issues, financial measures and CAP effects on cereal production and marketing.

  2. Reproduction, physiology and biochemistry

    Science.gov (United States)

    This chapter summarizes fundamental knowledge and recent discoveries about the reproduction, physiology and biochemistry of plant-parasitic nematodes. Various types of reproduction are reviewed, including sexual reproduction and mitotic and meiotic parthenogenesis. Although much is known about the p...

  3. Female reproductive disorders

    DEFF Research Database (Denmark)

    Crain, D Andrew; Janssen, Sarah J; Edwards, Thea M

    2008-01-01

    To evaluate the possible role of endocrine-disrupting compounds (EDCs) on female reproductive disorders emphasizing developmental plasticity and the complexity of endocrine-dependent ontogeny of reproductive organs. Declining conception rates and the high incidence of female reproductive...

  4. Normal Female Reproductive Anatomy

    Science.gov (United States)

    ... historical Searches are case-insensitive Reproductive System, Female, Anatomy Add to My Pictures View /Download : Small: 720x756 ... Large: 3000x3150 View Download Title: Reproductive System, Female, Anatomy Description: Anatomy of the female reproductive system; drawing ...

  5. Evaluation of a reproductive index to estimate grasshopper sparrow and eastern meadowlark reproductive success

    Science.gov (United States)

    Althoff, D.P.; Gipson, P.S.; Pontius, J.S.; Japuntich, R.D.

    2009-01-01

    We compared an index of reproductive success based on breeding behavior to actual nest fates of grasshopper sparrows (Ammodramus savannarum) and eastern meadowlarks (Sturnella magna) on 12 plots (4-ha). Concordance of results between the two methods was 58% for grasshopper sparrows and 42% for eastern meadowlarks on a plot-by-plot basis. The indirect method yielded higher estimates of reproductive activity than nest monitoring for the balance of the plots,. There was little evidence that brown-headed cowbird (Molothrus ater) parasitism influenced the estimates of reproductive success using the indirect method. We concluded that nests and about-to-fledge nestlings were missed during searches on some plots. It may be appropriate to use an indirect method to more efficiently survey territories and/or plots for species with hard-to-find nests or when monitoring large areas. Use of a reproductive index may be appropriate and more time-efficient than nest searching and monitoring for comparing management effects such as burning, grazing, haying, military training, and other localized disturbances that are likely to affect reproductive success of grasshopper sparrows and eastern meadowlarks. However, nest monitoring may be necessary for more precise estimates of productivity necessary for long-term monitoring. Nest monitoring results are also likely to allow for direct comparisons to results from other studies because the index method requires intimate knowledge of the species being evaluated - a factor that could lead to reduced precision because the experience level of technicians relying only on behavioral cues from study-to-study is likely to vary considerably.

  6. Evaluation of a Reproductive Index to Estimate Grasshopper Sparrow and Eastern Meadowlark Reproductive Success

    Directory of Open Access Journals (Sweden)

    D. Althoff

    2009-06-01

    Full Text Available We compared an index of reproductive success based on breeding behavior to actual nest fates of grasshopper sparrows (Ammodramus savannarum and eastern meadowlarks (Sturnella magna on 12 plots (4-ha. Concordance of results between the two methods was 58% for grasshopper sparrows and 42% for eastern meadowlarks on a plot-by-plot basis. The indirect method yielded higher estimates of reproductive activity than nest monitoring for the balance of the plots,. There was little evidence that brown-headed cowbird (Molothrus ater parasitism influenced the estimates of reproductive success using the indirect method. We concluded that nests and about-to-fledge nestlings were missed during searches on some plots. It may be appropriate to use an indirect method to more efficiently survey territories and/or plots for species with hard-to-find nests or when monitoring large areas. Use of a reproductive index may be appropriate and more time-efficient than nest searching and monitoring for comparing management effects such as burning, grazing, haying, military training, and other localized disturbances that are likely to affect reproductive success of grasshopper sparrows and eastern meadowlarks. However, nest monitoring may be necessary for more precise estimates of productivity necessary for long-term monitoring. Nest monitoring results are also likely to allow for direct comparisons to results from other studies because the index method requires intimate knowledge of the species being evaluated - a factor that could lead to reduced precision because the experience level of technicians relying only on behavioral cues from study-to-study is likely to vary considerably.

  7. An experimental study on fermentative H₂ production from food waste as affected by pH.

    Science.gov (United States)

    Cappai, G; De Gioannis, G; Friargiu, M; Massi, E; Muntoni, A; Polettini, A; Pomi, R; Spiga, D

    2014-08-01

    Batch dark fermentation experiments were performed on food waste and mixtures of food waste and wastewater activated sludge to evaluate the influence of pH on biological H2 production and compare the process performance with and without inoculum addition. The effect of a preliminary thermal shock treatment of the inoculum was also investigated as a means to harvest the hydrogenogenic biomass. The best performance in terms of both H2 generation potential and process kinetics was observed at pH=6.5 under all experimental conditions (no inoculum, and untreated or thermally treated inoculum added). H2 production from food waste was found to be feasible even without inoculum addition, although thermal pre-treatment of the inoculum notably increased the maximum production and reduced the lag phase duration. The analysis of the fermentation products indicated that the biological hydrogen production could be mainly ascribed to a mixed acetate/butyrate-type fermentation. However, the presence of additional metabolites in the digestate, including propionate and ethanol, also indicated that other metabolic pathways were active during the process, reducing substrate conversion into hydrogen. The plateau in H2 generation was found to mirror the condition at which soluble carbohydrates were depleted. Beyond this condition, homoacetogenesis probably started to play a role in the degradation process.

  8. Female reproductive disorders

    DEFF Research Database (Denmark)

    Crain, D Andrew; Janssen, Sarah J; Edwards, Thea M;

    2008-01-01

    To evaluate the possible role of endocrine-disrupting compounds (EDCs) on female reproductive disorders emphasizing developmental plasticity and the complexity of endocrine-dependent ontogeny of reproductive organs. Declining conception rates and the high incidence of female reproductive disrupti......To evaluate the possible role of endocrine-disrupting compounds (EDCs) on female reproductive disorders emphasizing developmental plasticity and the complexity of endocrine-dependent ontogeny of reproductive organs. Declining conception rates and the high incidence of female reproductive...

  9. Subordinate wasps are more aggressive in colonies with low reproductive skew

    DEFF Research Database (Denmark)

    Fanelli, D.; Boomsma, Jacobus Jan; Turillazzi, S.

    2008-01-01

    The small societies of primitively eusocial wasps have provided interesting testing grounds for reproductive skew theory because all individuals have similar reproductive potential, which is unusual in social insects but common in vertebrate societies. Aggression is a key parameter in testing...... the theory, but empirical studies have seldom quantified aggression together with the entire array of other relevant variables. The few studies that have done so were recently criticized for failing to control for the overall level of social activity. We analysed behaviour and reproductive partitioning...... patterns in the stenogastrine wasp Parischnogaster mellyi. We used aggression of the subordinate (ß) breeder as key variable and analysed how relatedness, body size, number of breeders and productivity affect the interaction between the reproductive skew and the aggression while controlling for nest...

  10. PREDICTING OF THE FACTORS AFFECTING CONSUMER BEHAVIOR THE CHOICE OF ORGANIC PRODUCTS BY LOGIT REGRESSION ANALYSIS

    Directory of Open Access Journals (Sweden)

    Selma KARABAŞ

    2012-06-01

    Full Text Available The purpose of this study is to determine consumer behavior towards organic products in Samsun city center and to determine the factors affacting their preferences of organic products. The study was interviewed with 478 consumers living the city center of Samsun. According of the findings, ease of accesibility of organic products, spouse’s educational level, paying extra for the organic produce, aware of food health benefits, considering the harmful effects of conventional produce, having complete knowledge of organic farming and one unit increase household number income to result increase consumption of organic produce. Consumers were not aware of the certification and control process. As a result, easy of accesibility of organic produces in the supermarkets need to be improved.

  11. Dirhinus giffardii (Hymenoptera: Chalcididae, parasitoid affecting Black Soldier Fly production systems in West Africa

    Directory of Open Access Journals (Sweden)

    Emilie Devic

    2015-12-01

    Full Text Available Interest for insect farming is currently growing globally. Conditions in West Africa appear suitable for developing such farming systems that can benefit communities by improving livelihoods, food and feed security or sanitation. In Ghana and Mali, the Black Soldier Fly (Hermetia illucens Linnaeus, 1758 is being produced for waste recycling and animal feed. In a two stages process (egg and larvae production, egg production was hampered by a pupal parasitoid, Dirhinus giffardii Silvestri, 1913 (Hymenoptera: Chalcididae, which reduced future broodstock by almost 72%. This is the first time D. giffardii is reported as a parasitoid of H. illucens pupae and one of the first reports of parasitism in this commercially important fly species. The introduction of precautionary measures is highly recommended for the success of H. illucens production systems in West Africa.

  12. Process parameters affecting the sustainability of fermentative hydrogen production: A short-review

    Directory of Open Access Journals (Sweden)

    Carlos Dinamarca, Rune Bakke

    2011-11-01

    Full Text Available Anaerobic fermentation is regarded as the least energy intensive method for H2 production. Extensive literature on experimental attempts to achieve the highest possible theoretical yield (e.g. 4 mol H2/mol glucose is available. All published steady state, mixed culture studies show yields much lower than the theoretical maximums for the substrates applied. This article summarizes the influence of key process parameters (pH and buffer systems, temperature, H2 partial pressure, feed stock, and reactor configuration on fermentative hydrogen production. The following three requirements for successful Bio-H2 fermentation in mixed cultures are identified: (1 Maintain environmental conditions for the formation of oxidized products; (2 Optimize the relationship between biomass and hydrogen yields; and (3 Maintain unfavorable conditions for hydrogen consuming organisms. Fulfilling these requirements has not yet been achieved in stable continuous cultures, and it may not be achievable do to some fundamental limitation.

  13. Engaged or exhausted—How does it affect dentists’ clinical productivity?

    Directory of Open Access Journals (Sweden)

    Jari J. Hakanen

    2014-06-01

    Full Text Available This cross-sectional study examines whether job burnout (exhaustion and work engagement are associated with the clinical productivity of dentists measured by the amount of paid procedure fees in a single month. We conducted an OLS regression analyses of data on dentists working at municipal health centers in Finland (N = 269; response rate 37%. The results indicated that work engagement was positively associated with the amount of procedure fees and consequently with dentists’ pay level after several work-related and demographic background variables were controlled for. However, exhaustion was not related to productivity after controlling for the impact of other factors.

  14. MODE OF PRODUCTION AND EDUCATION: CONSIDERATIONS ABOUT EDUCATION IN THE CAPITALIST REPRODUCTION AND IN THE TRANSITION TO THE SOCIALISM

    Directory of Open Access Journals (Sweden)

    Patrícia Vieira Trópia

    2009-06-01

    Full Text Available This text works about the relation between mode of production and education, from the contributions of the althusseriana view. The contributions of this approach allow the overcoming review to the economicist and reductionist view about the kind of articulation between the base/economic structure and the political and ideological superstructure and its implications for education. From this point of view, the school, the school education, is really a place of contradiction. However, any internal transformation to the school to make it, in fact, a school to serve the working classes would demand a breakup with the organic commitment of the educational unit. But from different approaches, economism and structuralism, come the question: “although some criticisms to the traps of the non-economist reviews are pertinent, cannot the rejection of the tests developed by the ‘sociological criticism of the education’ in the educational field put us in so many others and more devilish traps, such as those from idealism and reformism?”.

  15. Cultivar, harvest date, and nitrogen fertilization affect production and quality of fall oat

    Science.gov (United States)

    Previous research has shown that oat (Avena sativa L.) has promise as a fall-forage option for dairy producers. In addition, dairy producers often have a recurring need to identify opportunity windows for manure hauling other than before or after production of corn (Zea mays L.). Our objectives were...

  16. Market oriented new product development: How can a means-end chain approach affect the process?

    DEFF Research Database (Denmark)

    Søndergaard, Helle Alsted

    Few disagree on the advantage of market oriented product development. However, can a well-known theory on consumer behaviour be used as a catalyst for achieving it? This paper describes a case study where means-end chain (MEC) approach was introduced to a cross-functional development team at two...

  17. Diversity of condensed tannin structures affects rumen in vitro methane production in sainfoin (Onobrychis viciifolia) accessions

    NARCIS (Netherlands)

    Hatew, B.; Hayot Carbonero, C.; Stringano, E.; Sales, L.F.; Smith, L.M.J.; Mueller-Harvey, I.; Hendriks, W.H.; Pellikaan, W.F.

    2015-01-01

    Sainfoin is a non-bloating temperate forage legume with a moderate-to-high condensed tannin (CT) content. This study investigated whether the diversity of sainfoin accessions in terms of CT structures and contents could be related to rumen in vitro gas and methane (CH4) production and fermentation c

  18. Structural features of condensed tannins affect in vitro ruminal methane production and fermentation characteristics

    NARCIS (Netherlands)

    Huyen, N.T.; Fryganas, C.; Uittenbogaard, G.; Mueller-Harvey, I.; Verstegen, M.W.A.; Hendriks, W.H.; Pellikaan, W.F.

    2016-01-01

    An in vitro study was conducted to investigate the effects of condensed tannin (CT) structural properties, i.e. average polymer size (or mean degree of polymerization), percentage of cis flavan-3-ols and percentage of prodelphinidins in CT extracts on methane (CH4) production and ferme

  19. Statistical analysis of factors affecting crop production in Navrongo, Tono irrigation dam a case study

    Directory of Open Access Journals (Sweden)

    D. JAKPERIK

    2013-02-01

    Full Text Available This study identified the essential factors of production in the Tono irrigation dam of the Upper East Region. The accessibility and patronage of these factors by farmers in this area was studied and how they influence crop production in the Region. A total of two hundred questionnaires designed by the Ministry of Food and Agriculture for farmers in Navrongo were administered. A snowball sampling design was employed to identify farmers on these facilities in the study area. Pearson correlation coefficient, principal component analysis, and subset regression analysis were used to unveil the relevant information in the study. The results revealed a high correlation between the factors of production being studied with each pair having a probability level less than 0.0001. The full general linear model was highly significant (F=662.50, psmaller tahn 0.0001 with only two factors (Farm size and Fertilizer accounting for 98.86% of the total variation in yield. This is a clear indication of multicollinearity and a subset regression analysis was used to identify the best subset that improves yield in the irrigation dam. The best subset comprised of Age, Farm size, seed, and Fertilizer accounting for 97.75% of the total variation in crop production in Navrongo. To enhance yield in Navrongo therefore, high yielding seeds, timely granting of fertilizer credit to farmers who mature enough and responsible with reasonable farm sizes should be encouraged.

  20. Intestinal microbial affects of yeast products on weaned and transport stressed pigs

    Science.gov (United States)

    Study objectives were to determine effects of a commercially available yeast product (XPC, Diamond-V Mills) and stress of transportation on total Enterobacteriaceae, Escherichia coli, coliforms, and Lactobacilli populations in the intestine of weaning pigs. In a RCB design with a 2 x 2 factorial ar...

  1. How forest management affects ecosystem services, including timber production and economic return

    DEFF Research Database (Denmark)

    Duncker, Philipp S.; Raulund-Rasmussen, Karsten; Gundersen, Per;

    2012-01-01

    Forest ecosystems deliver multiple goods and services and, traditionally, forest owners tend to have a high interest in goods in the form of merchantable wood. As a consequence, forest management often aims to increase timber production and economic returns through intervention into natural...

  2. Microvesicle formulations used in topical drugs and cosmetics affect product efficiency, performance and allergenicity

    DEFF Research Database (Denmark)

    Madsen, Jakob Torp; Ejner Andersen, Klaus

    2010-01-01

    Attempts to improve the formulations of topical products are continuing processes (ie, to increase cosmetic performance, enhance effects, and protect ingredients from degradation). The development of micro- and nanovesicular systems has led to the marketing of topical drugs and cosmetics that use...

  3. Soil water infiltration affected by biofuel and grain crop production systems in claypan landscape

    Science.gov (United States)

    The effect of soil management systems on water infiltration is very crucial within claypan landscapes to maximize production as well as minimize environmental risks. The objective of this study was to assess the effect of topsoil thickness on water infiltration in claypan soils for grain and biofuel...

  4. Boosting in planta production of antigens derived from the porcine reproductive and respiratory syndrome virus (PRRSV) and subsequent evaluation of their immunogenicity.

    Science.gov (United States)

    Piron, Robin; De Koker, Stefaan; De Paepe, Annelies; Goossens, Julie; Grooten, Johan; Nauwynck, Hans; Depicker, Ann

    2014-01-01

    Porcine reproductive and respiratory syndrome (PRRS) is a disease of swine, caused by an arterivirus, the PRRS virus (PRRSV). This virus infects pigs worldwide and causes huge economic losses. Due to genetic drift, current vaccines are losing their power. Adaptable vaccines could provide a solution to this problem. This study aims at producing in planta a set of antigens derived from the PRRSV glycoproteins (GPs) to be included in a subunit vaccine. We selected the GP3, GP4 and GP5 and optimized these for production in an Arabidopsis seed platform by removing transmembrane domains (Tm) and/or adding stabilizing protein domains, such as the green fluorescent protein (GFP) and immunoglobulin (IgG) 'Fragment crystallizable' (Fc) chains. Accumulation of the GPs with and without Tm was low, reaching no more than 0.10% of total soluble protein (TSP) in homozygous seed. However, addition of stabilizing domains boosted accumulation up to a maximum of 2.74% of TSP when GFP was used, and albeit less effectively, also the Fc chains of the porcine IgG3 and murine IgG2a increased antigen accumulation, to 0.96% and 1.81% of TSP respectively, while the murine IgG3 Fc chain did not. Antigens with Tm were less susceptible to these manipulations to increase yield. All antigens were produced in the endoplasmic reticulum and accordingly, they carried high-mannose N-glycans. The immunogenicity of several of those antigens was assessed and we show that vaccination with purified antigens did elicit the production of antibodies with virus neutralizing activity in mice but not in pigs.

  5. Boosting in planta production of antigens derived from the porcine reproductive and respiratory syndrome virus (PRRSV and subsequent evaluation of their immunogenicity.

    Directory of Open Access Journals (Sweden)

    Robin Piron

    Full Text Available Porcine reproductive and respiratory syndrome (PRRS is a disease of swine, caused by an arterivirus, the PRRS virus (PRRSV. This virus infects pigs worldwide and causes huge economic losses. Due to genetic drift, current vaccines are losing their power. Adaptable vaccines could provide a solution to this problem. This study aims at producing in planta a set of antigens derived from the PRRSV glycoproteins (GPs to be included in a subunit vaccine. We selected the GP3, GP4 and GP5 and optimized these for production in an Arabidopsis seed platform by removing transmembrane domains (Tm and/or adding stabilizing protein domains, such as the green fluorescent protein (GFP and immunoglobulin (IgG 'Fragment crystallizable' (Fc chains. Accumulation of the GPs with and without Tm was low, reaching no more than 0.10% of total soluble protein (TSP in homozygous seed. However, addition of stabilizing domains boosted accumulation up to a maximum of 2.74% of TSP when GFP was used, and albeit less effectively, also the Fc chains of the porcine IgG3 and murine IgG2a increased antigen accumulation, to 0.96% and 1.81% of TSP respectively, while the murine IgG3 Fc chain did not. Antigens with Tm were less susceptible to these manipulations to increase yield. All antigens were produced in the endoplasmic reticulum and accordingly, they carried high-mannose N-glycans. The immunogenicity of several of those antigens was assessed and we show that vaccination with purified antigens did elicit the production of antibodies with virus neutralizing activity in mice but not in pigs.

  6. Microsatellite analysis of the parental contribution of Piaractus mesopotamicus to the production of offspring in the semi-natural system of reproduction

    Directory of Open Access Journals (Sweden)

    Jayme Aparecido Povh

    2010-04-01

    Full Text Available The objective of this study was to evaluate the genetic diversity and the parental contribution of Piaractus mesopotamicus in the production of offspring in the semi-natural system of reproduction. Twenty parental fishes (eleven males and nine females and the total of 100 larvae were evaluated by microsatellite marker. The parents and offspring had thirty-one alleles and heterozygosity of 0.550 and 0.563, respectively. The females were fertilised by two up to six males while the males fertilised three up to five females. The contribution of the females and males to the offspring were 66.6 and 58%, respectively. Such results indicated no loss in the genetic variability in the offspring, and the parents had multiple paternity and reasonable contribution to the offspring production.O objetivo deste trabalho foi avaliar a diversidade genética e a contribuição parental de Piaractus mesopotamicus na produção de descendência no sistema seminatural de reprodução. Vinte peixes parentais (onze machos e nove fêmeas e o total de 100 larvas foram avaliados por meio do marcador microssátelite. Os parentais e a progênie tiveram trinta e um alelos e heterozigosidade de 0,550 e 0,563, respectivamente. As fêmeas foram fertilizadas por dois até seis machos enquanto machos fertilizaram três até cinco fêmeas. A contribuição de fêmeas e machos para a descendência seja 66,6 e 58,0%, respectivamente. Tais resultados não indicam diminuição da variabilidade genética na progênie e os parentais apresentaram paternidade múltipla e razoável contribuição à produção de descendência.

  7. Grandparental effects on reproductive strategizing

    Directory of Open Access Journals (Sweden)

    2004-09-01

    Full Text Available This paper analyzes data from the household registers for two villages in the Nôbi region of central Japan in the late Edo period (1717-1869 to assess how grandparents may have affected reproductive strategizing in stem families. The particulars of the family system fostered a culturally favored set of reproductive goals, in particular, a daughter as eldest child, followed by a son (and heir, coupled with gender alternation in subsequent reproduction and overall gender balance. This reproductive strategy was generally followed during the stem phase of the domestic cycle, when one or both grandparents were present, especially when the family head was in the senior generation. By contrast, a son-first strategy was favored when childbearing began in the conjugal phase of the cycle. This suggests grandparental influence on the junior couple's reproductive decisions in favor of the cultural ideal. I find that the senior couple's decision to marry the heir early or late strongly affects the reproductive strategies followed by him after marriage. I show that when a grandmother is present at the onset of childbearing, especially if she is relatively young, the junior couple ends up with more offspring on average. A controlled analysis of infanticiding behavior is interpreted in terms of conjugal power and coalition formation. It appears that a grandmother gets her way only when she and her son gang up on the daughter-in-law, but such a coalition is likely only when her son dominates the conjugal relationship (which in turn reflects the grandmother's success in binding the son tightly to her emotionally and in delaying his marriage. Otherwise, the grandmother may be shut out from reproductive decision-making by the solidary conjugal coalition.

  8. Altered reproduction in fish exposed to pulp and paper mill effluents: roles of individual compounds and mill operating conditions.

    Science.gov (United States)

    Hewitt, L Mark; Kovacs, Tibor G; Dubé, Monique G; MacLatchy, Deborah L; Martel, Pierre H; McMaster, Mark E; Paice, Michael G; Parrott, Joanne L; van den Heuvel, Michael R; van der Kraak, Glen J

    2008-03-01

    For the last 20 years, studies conducted in North America, Scandinavia, and New Zealand have shown that pulp and paper mill effluents affect fish reproduction. Despite the level of effort applied, few leads are available regarding the factors responsible. Effluents affect reproduction in multiple fish species, as evidenced by decreased gonad size, decreased circulating and gonadal production of reproductive steroids, altered expression of secondary sex characteristics, and decreased egg production. Several studies also have shown that effluent constituents are capable of accumulating in fish and binding to sex steroid receptors/ binding proteins. Studies aimed at isolating biologically active substances within the pulping and papermaking process have provided clues about their source, and work has progressed in identifying opportunities for in-mill treatment technologies. Following comparisons of manufacturing processes and fish responses before and after process changes, it can be concluded that effluent from all types of mill processes are capable of affecting fish reproduction and that any improvements could not be attributed to a specific process modification (because mills normally performed multiple modifications simultaneously). Improved reproductive performance in fish generally was associated with reduced use of molecular chlorine, improved condensate handling, and liquor spill control. Effluent biotreatment has been effective in reducing some effects, but biotreated effluents also have shown no difference or an exacerbation of effects. The role of biotreatment in relation to effects on fish reproduction remains unclear and needs to be resolved.

  9. Enterococcus faecium isolated from Lombo, a Portuguese traditional meat product: characterisation of antibacterial compounds and factors affecting bacteriocin production.

    Science.gov (United States)

    Todorov, S D; Favaro, L; Gibbs, P; Vaz-Velho, M

    2012-12-01

    Strain ST211CH, identified as a strain of Enterococcus faecium, isolated from Lombo produced a bacteriocin that inhibited the growth of Enterococcus spp., Listeria spp., Klebsiella spp., Lactobacillus spp., Pseudomonas spp., Staphylococcus spp. and Streptococcus spp. The mode of action of the bacteriocin named as bacteriocin ST211Ch was bactericidal against Enterococcus faecalis ATCC19443. As determined by Tricine-SDS-PAGE, the approximate molecular mass of the bacteriocin was 8.0 kDa. Loss in antimicrobial activity was recorded after treatment with proteolytic enzymes. Maximum activity of bacteriocin ST211Ch was measured in broth cultures of E. faecium strain ST211Ch after 24 h; thereafter, the activity was reduced. Bacteriocin ST211Ch remained active after exposure to various temperatures and pHs, as well as to Triton X-100, Tween-80, Tween-20, sodium dodecyl sulfate, NaCl, urea and EDTA. Effect of media components on production of bacteriocin ST211Ch was also studied. On the basis of PCR reactions targeting different bacteriocin genes, i.e. enterocins, curvacins and sakacins, no evidences for the presence of these genes in the total DNA of E. faecium strain ST211Ch was obtained. The bacterium most probably produced a bacteriocin different from those mentioned above. Based on the antimicrobial spectrum, stability and mode of action of bacteriocin ST211CH, E. faecium strain ST211Ch might be considered as a potential candidate with beneficial properties for use in biopreservation to control food spoilage bacteria.

  10. Do new cellulolytic enzyme preparations affect the industrial strategies for high solids lignocellulosic ethanol production?

    DEFF Research Database (Denmark)

    Cannella, David; Jørgensen, Henning

    2014-01-01

    Production of ethanol from lignocellulosic materials has a promising market potential, but the process is still only at pilot/demonstration scale due to the technical and economical difficulties of the process. Operating the process at very high solids concentrations (above 20% dry matter—DM) has...... matter conditions. In this work the impact of selected enzyme preparation and processing strategy (SHF, presaccharification and simultaneous saccharification and fermentation—PSSF, and SSF) on final ethanol yield and overall performance was investigated with pretreated wheat straw up to 30% DM....... The experiments revealed that an SSF strategy was indeed better than SHF when applying an older generation enzyme cocktail (Celluclast-Novozym 188). In case of the newer product Cellic CTec 2, SHF resulted in 20% higher final ethanol yield compared to SSF. It was possible to close the mass balance around...

  11. The Major Recessive Calamities Affecting the Wheat Production in Chuxiong Prefecture and Control Countermeasures

    Institute of Scientific and Technical Information of China (English)

    Maochang; YANG

    2014-01-01

    The impact of recessive calamities was analyzed,including seasonal drought,cold injury,dry hot wind and aphid in the wheat production of Chuxiong Prefecture,and the countermeasures that prevented and controlled the recessive calamities in a target-oriented way were proposed,including the improvement of basic farmland,the application of organic manure,the promotion of the breed with high stress resistance,the seedling at suitable date,the improvement of control on fertilizing and watering,the enhancement of management on cultivating and controlling disease in time,and the breeding new variety adaptive to local ecosystem,in order to advance the wheat production in a sustainable way.

  12. How Can Diet Affect the Accumulation of Advanced Glycation End-Products in the Human Body?

    Directory of Open Access Journals (Sweden)

    Axel Guilbaud

    2016-12-01

    Full Text Available The accumulation of advanced glycation end products (AGEs is associated with the complications of diabetes, kidney disease, metabolic disorders and degenerative diseases. It is recognized that the pool of glycation products found in the human body comes not only from an endogenous formation, but also from a dietary exposure to exogenous AGEs. In recent years, the development of pharmacologically-active ingredients aimed at inhibiting endogenous glycation has not been successful. Since the accumulation of AGEs in the human body appears to be progressive throughout life, an early preventive action against glycation could be effective through dietary adjustments or supplementation with purified micronutrients. The present article provides an overview of current dietary strategies tested either in vitro, in vivo or both to reduce the endogenous formation of AGEs and to limit exposure to food AGEs.

  13. The complex interaction between obesity, metabolic syndrome and reproductive axis: a narrative review.

    Science.gov (United States)

    Michalakis, Konstantinos; Mintziori, Gesthimani; Kaprara, Athina; Tarlatzis, Basil C; Goulis, Dimitrios G

    2013-04-01

    The aim of this narrative review is to provide current evidence for the interaction between obesity, metabolic syndrome (MS) and reproductive axis. Gonadotropin-releasing hormone (GnRH) pulses and, consequently, normal function of reproductive (hypothalamus-pituitary-gonadal) axis depend on normal energy balance, which presupposes sufficient food intake, reasonable energy consumption and average thermoregulatory costs. In case of an energy imbalance, reproductive dysfunction may occur. In young women, excessive leanness is accompanied by puberty delay, whereas premature puberty might be a manifestation of obesity. In a similar way, obesity in men affects fertility. Excess adipose tissue results in increased conversion of testosterone to estradiol, which may lead to secondary hypogonadism through reproductive axis suppression. Moreover, oxidative stress at the level of the testicular micro-environment may result in decreased spermatogenesis and sperm damage. Products of the adipocyte, such as leptin, adiponectin and resistin, and gut peptides, such as ghrelin, are considered to be crucial in the interaction between energy balance and reproduction. Finally, an indirect evidence for the interplay between MS and reproductive axis is the fact that when treating components of one, parameters of the other can be improved as well. These therapeutic interventions include lifestyle modifications, pharmacological agents, such as sex hormone replacement therapy, and surgical procedures. Although many issues remain unclear, the elucidation of the complex interaction between MS and reproductive axis will have obvious clinical implications in the therapeutic approach of both entities.

  14. Analysis of the Factors Affecting Consumer’s Some Traditional Food Products Preferences by Multidimensional Scaling Method

    Directory of Open Access Journals (Sweden)

    Y. Oraman G. Unakıtan E. Yılmaz B. Başaran

    2011-01-01

    Full Text Available The aim of this study is to evaluate consumer behaviour towards factors affecting purchase decision of some traditional food products and grouping those consumer attitudes. The original data was obtained from results of a survey conducted in 14 different districts in Tekirdag. The survey consisted of face-to-face interviews conducted within a total sample of 166 households randomly. In the study, multidimensional scaling analysis is used for evaluating the effective factors of consumer preferences for traditional products and grouping their preferences. It is found that taste, food safety and freshness have similar effects on consumer preferences for yogurt, molasses and noodles. Price has an important and positive loaded effect on other variables for the three products.

  15. Rethinking Food: How United States Agriculture Production Affects Security Policy and Global Markets

    Science.gov (United States)

    2014-06-13

    of re- exporting , it is assumed some countries (like Argentina did) must have done so for either economic or political gain with the Soviets. Following... Argentina and Brazil began exporting soy meal in 1977. That same year Brazil displaced America as the top soy meal exporter in the world while Argentina ...likely prevent an ideal distribution of food. The United States has been and remains a major exporter of several key commodities giving US production

  16. The interactive effects of affect and shopping goal on information search and product evaluations.

    Science.gov (United States)

    Chen, Fangyuan; Wyer, Robert S; Shen, Hao

    2015-12-01

    Although shoppers often want to evaluate products to make a purchase decision, they can also shop for enjoyment. In each case, the amount of time they spend on shopping and the number of options they consider can depend on the mood they happen to be in. We predicted that mood can signal whether the goal has been attained and when people should stop processing information. When people are primarily motivated to purchase a particular type of product, positive mood signals that they have done enough. Thus, they consider less information if they are happy than if they are unhappy. When people shop for enjoyment, however, positive mood signals that they are still having fun. Thus, they consider more information when they are happy than when they are not. Four experiments among university students (N = 827) examined these possibilities. Experiment 1 provided initial evidence for the interactive effects of mood and goals on search behavior and product evaluation. Other studies examined the implications of this conceptualization for different domains: (a) the relative impact of brand and attribute information on judgments (Experiment 2), (b) gender differences in shopping behavior (Experiment 3), and (c) the number of options that people review in an actual online shopping website (Experiment 4).

  17. Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii.

    Science.gov (United States)

    Silva, Rui; Aguiar, Tatiana Q; Domingues, Lucília

    2015-01-10

    The Ashbya gossypii riboflavin biosynthetic pathway and its connection with the purine pathway have been well studied. However, the outcome of genetic alterations in the pyrimidine pathway on riboflavin production by A. gossypii had not yet been assessed. Here, we report that the blockage of the de novo pyrimidine biosynthetic pathway in the recently generated A. gossypii Agura3 uridine/uracil auxotrophic strain led to improved riboflavin production on standard agar-solidified complex medium. When extra uridine/uracil was supplied, the production of riboflavin by this auxotroph was repressed. High concentrations of uracil hampered this (and the parent) strain growth, whereas excess uridine favored the A. gossypii Agura3 growth. Considering that the riboflavin and the pyrimidine pathways share the same precursors and that riboflavin overproduction may be triggered by nutritional stress, we suggest that overproduction of riboflavin by the A. gossypii Agura3 may occur as an outcome of a nutritional stress response and/or of an increased availability in precursors for riboflavin biosynthesis, due to their reduced consumption by the pyrimidine pathway.

  18. The yeast TUM1 affects production of hydrogen sulfide from cysteine treatment during fermentation.

    Science.gov (United States)

    Huang, Chien-Wei; Walker, Michelle E; Fedrizzi, Bruno; Roncoroni, Miguel; Gardner, Richard C; Jiranek, Vladimir

    2016-12-01

    The undesirable rotten-egg odour of hydrogen sulfide (H2S) produced by yeast shortly after yeast inoculation of grape musts might be an important source of desirable varietal thiols, which contribute to tropical aromas in varieties such as Sauvign-on Blanc. In this study, we observed that Saccharomyces cerevisiae strains produce an early burst of H2S from cysteine. Both Δmet2 and Δmet17 strains produce a larger burst, likely because they are unable to utilise the H2S in the sulfate assimilation pathway. For the first time, we show that TUM1 is partly responsible for the early production of H2S from cysteine. Overex-pressing TUM1 elevated production of H2S, whilst its deletion yields only half of the H2S. We further confirmed that yeast convert cysteine to H2S by analysing growth of mutants lacking components of the transsulfuration pathway. High concent-rations of cysteine overcame this growth block, but required TUM1 Collectively, the data indicate that S. cerevisiae does not convert cysteine to sulfate or sulfite, but rather to sulfide via a novel pathway that requires the action of Tum1p. The findi-ngs of this study may allow the improvement of commercial yeasts through the manipulation of sulfur metabolism that are better suited towards production of fruit-driven styles.

  19. Identification of significant medium components that affect docosahexaenoic acid production by Schizochytrium sp. SW1

    Science.gov (United States)

    Manikan, Vidyah; Hamid, Aidil A.

    2013-11-01

    Central composite design (CCD) was employed to investigate the significance of glucose, yeast extract, MSG and sea salt in affecting the amount of docosahexaenoic acid (DHA) accumulated by a locally isolated strain of Schizochytrium. Design Expert software was used to construct a set of experiments where each medium component mentioned above was varied over three levels. Cultivation was carried out in 250mL flasks containing 50mL of medium, incubated at 30°C with 200 rpm agitation for 96 hours. ANOVA was conducted to identify the influential factors and the level of their significance where factors that scored a probability value of less than 0.05 were considered significant. The level of influence for each independent variable was also interpreted using perturbation whereas pattern of interaction between the factors were interpreted using interaction plots. This experiment revealed that yeast extract and monosodium glutamate have significant influence on DHA accumulation process by Schizochytrium sp. SW1.

  20. Social Factors Affecting Wetlands Utilization for Agriculture in Nigeria: A case study of sawah rice production

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Wetlands have immense poverty-fighting potentials and in Nigeria,more and more people are dependent on wetlands for their livelihoods.To examine the social factors affecting the current status of the wetlands utilization for agriculture in Nigeria,a simple random sampling technique was used to select 200 farmers cultivating wetlands and a structured questionnaire was applied to elicit the information on the social factors.Data collected were described using frequency and percentage and a multiple regression analysis was used to identify significant variables that are determinants of wetland utilization.The results of the analysis showed that significant variables included crop preferences,farming system,culture,taste,land tenure,knowledge of wetland cultivation,perceived suitability,farmers' tribe,location of wetland,and farmers' age.It was concluded with suggestions for the right combination of policies,public awareness,and appropriate farming methods in order to improve wetland utilization in Nigeria.

  1. Do new cellulolytic enzyme preparations affect the industrial strategies for high solids lignocellulosic ethanol production?

    Science.gov (United States)

    Cannella, David; Jørgensen, Henning

    2014-01-01

    Production of ethanol from lignocellulosic materials has a promising market potential, but the process is still only at pilot/demonstration scale due to the technical and economical difficulties of the process. Operating the process at very high solids concentrations (above 20% dry matter-DM) has proven essential for economic feasibility at industrial scale. Historically, simultaneous saccharification and fermentation (SSF) was found to give better ethanol yields compared to separate hydrolysis and fermentation (SHF), but data in literature are typically based on operating the process at low dry matter conditions. In this work the impact of selected enzyme preparation and processing strategy (SHF, presaccharification and simultaneous saccharification and fermentation-PSSF, and SSF) on final ethanol yield and overall performance was investigated with pretreated wheat straw up to 30% DM. The experiments revealed that an SSF strategy was indeed better than SHF when applying an older generation enzyme cocktail (Celluclast-Novozym 188). In case of the newer product Cellic CTec 2, SHF resulted in 20% higher final ethanol yield compared to SSF. It was possible to close the mass balance around cellulose to around 94%, revealing that the most relevant products could be accounted for. One observation was the presence of oxidized sugar (gluconic acid) upon enzymatic hydrolysis with the latest enzyme preparation. Experiments showed gluconic acid formation by recently discovered enzymatic class of lytic polysaccharides monoxygenases (LPMO's) to be depending on the processing strategy. The lowest concentration was achieved in SSF, which could be correlated with less available oxygen due to simultaneous oxygen consumption by the yeast. Quantity of glycerol and cell mass was also depending on the selected processing strategy.

  2. Evaluation of Factors Affecting Polyhydroxyalkanoates Production by Comamonas sp. EB172 Using Central Composite Design

    Directory of Open Access Journals (Sweden)

    Noor Azman Mohd Johar

    2012-09-01

    Full Text Available Aims: Statistical approach, central composite design (CCD was used to investigate the complex interaction among temperature (25-37 °C, initial medium pH (5-9, inoculum size (4-10 % (v/v, concentration of (NH42SO4 (0-1 g/L and concentration of mixed organic acids (5-10 g/L in the production of polyhydroxyalkanoates by Comamonas sp. EB172.Methodology and Results: Mixed organic acids derived from anaerobically treated palm oil mill effluent (POME containing acetic:propionic:butyric (ratio of 3:1:1 were used as carbon source in the batch culture of Comamonas sp. EB172 to produce polyhydoxyalkanoates (PHAs. The analysis of variance (ANOVA showed that all five factors were significantly important in the batch fermentation by shake flask with a P value of less than 0.001. The optimal temperature, initial medium pH, inoculum size, concentration of (NH42SO4 and concentration of mixed organic acids were 30 °C, 7.04, 4.0 % (v/v, 0.01 g/L and 5.05 g/L respectively.Conclusion, significance and impact of study: Optimization of the production medium containing mixed organic acids has improved the PHA production for more than 2 folds. Under optimal condition in the shake flask fermentation, the predicted growth is 2.98 g/L of dry cell weight (DCW with 47.07 wt % of PHA content. The highest yield of PHA was 0.28 g of PHA per g mixed organic acids.

  3. Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production.

    Science.gov (United States)

    Vistica, D T; Skehan, P; Scudiero, D; Monks, A; Pittman, A; Boyd, M R

    1991-05-15

    The hydrogen acceptor 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) is commonly utilized to estimate cellular viability in drug screening protocols. The present investigation was prompted, in part, by observations that reduction of MTT to its colored reaction product, MTT formazan, varied between cell lines and with culture age. A correlation was established between the D-glucose concentration of the culture medium at the time of assay and the production of MTT formazan for cell lines representing seven tumor histologies. A decrease in the concentration of D-glucose from culture medium was accompanied by a decrease in MTT specific activity (MTT formazan/microgram cell protein) for a number of cell lines. Cells which extensively metabolized D-glucose exhibited the greatest reduction in MTT specific activity. Further evidence that the D-glucose concentration of the culture medium played an important role in MTT reduction was provided by experiments which demonstrated that transfer of cells to a glucose-free medium (L-15) was accompanied by an immediate decrease in MTT reduction which was pH independent. These studies suggested that cellular transport and constant metabolism of glucose were required for maximum MTT reduction. Decreases in the cellular concentration of the reduced pyridine nucleotides NADH and NADPH were accompanied by concomitant decreases in MTT formazan production. MTT formazan varied significantly among cell lines in both the kinetics of its formation and the degree of saturability exhibited. Apparent IC50 values for Adriamycin varied, in a cell line-specific manner, with MTT exposure time. These results indicate that MTT specific activity is significantly influenced by a number of parameters and suggest that assay conditions should be established which minimize their effects.

  4. Nutrient availability affects pigment production but not growth in lichens of biological soil crusts

    Science.gov (United States)

    Bowker, M.A.; Koch, G.W.; Belnap, J.; Johnson, N.C.

    2008-01-01

    Recent research suggests that micronutrients such as Mn may limit growth of slow-growing biological soil crusts (BSCs) in some of the drylands of the world. These soil surface communities contribute strongly to arid ecosystem function and are easily degraded, creating a need for new restoration tools. The possibility that Mn fertilization could be used as a restoration tool for BSCs has not been tested previously. We used microcosms in a controlled greenhouse setting to investigate the hypothesis that Mn may limit photosynthesis and consequently growth in Collema tenax, a dominant N-fixing lichen found in BSCs worldwide. We found no evidence to support our hypothesis; furthermore, addition of other nutrients (primarily P, K, and Zn) had a suppressive effect on gross photosynthesis (P = 0.05). We also monitored the growth and physiological status of our microcosms and found that other nutrients increased the production of scytonemin, an important sunscreen pigment, but only when not added with Mn (P = 0.01). A structural equation model indicated that this effect was independent of any photosynthesis-related variable. We propose two alternative hypotheses to account for this pattern: (1) Mn suppresses processes needed to produce scytonemin; and (2) Mn is required to suppress scytonemin production at low light, when it is an unnecessary photosynthate sink. Although Mn fertilization does not appear likely to increase photosynthesis or growth of Collema, it could have a role in survivorship during environmentally stressful periods due to modification of scytonemin production. Thus, Mn enrichment should be studied further for its potential to facilitate BSC rehabilitation. ?? 2008 Elsevier Ltd.

  5. Magnetic nanowires and hyperthermia: How geometry and material affect heat production efficiency

    KAUST Repository

    Contreras, Maria F.

    2015-05-01

    Magnetic hyperthermia, which refers to the production of heat by magnetic nanostructures under an alternating magnetic field (AMF), has been previously investigated with superparamagnetic nanobeads as a cancer therapy method. Magnetic nanowires (NWs) used in hyperthermia can be very promising, as it has been shown that they have a larger magnetic moment per unit of volume compared to the nanobeads. Moreover, Fe NWs proved to have a higher heating efficiency compared to Fe nanobeads, when exposed to an AMF at the same concentration [1].

  6. Evaluation of Factors Affecting Uricase Production by the Screened Wild/Natural Microbes

    OpenAIRE

    Harinath Dwivedi; Kusum Agrawal; Saraf, Shubhini A.

    2012-01-01

    Gout is a metabolic disorder due to the deposition of uric acid crystals within articular or periarticular tissues. Uricase (urate oxidase) catalyzes the oxidation of less water soluble uric acid (7 mg/dl) to a compound allantoin which is more water soluble(11g/L at 40°C) resulting into the ease of excretion of uric acid. The objective of the work was to develop a new method for screening of microbes for uricase production and estimation of uricase thereof. This was achieved by utilizing the ...

  7. TNT transformation products are affected by the growth conditions of Raoultella terrigena.

    Science.gov (United States)

    Claus, Harald; Perret, Nina; Bausinger, Tobias; Fels, Gregor; Preuss, Johannes; König, Helmut

    2007-03-01

    High concentrations of 2,4,6-trinitrotoluene (TNT) and related nitroaromatic compounds are commonly found in soil and groundwater at former explosive plants. The bacterium, Raoultella terrigena strain HB, isolated from a contaminated site, converts TNT into the corresponding amino products. Radio-HPLC analysis with [(14)C]TNT identified aminodinitrotoluene, diaminonitrotoluene and azoxy-dimers as the main metabolites. Transformation rate and the type of metabolites that predominated in the culture medium and within the cells were significantly influenced by the culture conditions. The NAD(P)H-dependent enzymatic reduction of nitro-substituted compounds by cell-free extracts of R. terrigena was evaluated in vitro.

  8. Microbiological and physicochemical factors affecting Aspergillus section Flavi incidence in Cavendish banana (Musa cavendishii) chips production in Southern Philippines.

    Science.gov (United States)

    Sales, A C; Azanza, P V; Yoshizawa, T

    2005-01-01

    Microbiological and physicochemical factors affecting the incidence of Aspergillus section Flavi in dried Cavendish banana (Musa cavendishii) chips production in Southern Philippines were examined. The average counts of Aspergillus section Flavi (AFC) in fresh and dried Cavendish bananas from 10 production batches of the Philippine Agro-Industrial Development Cooperative in Davao del Norte, Southern Philippines were 1.2 x 10(2) and 1.6 x 10(2) cfu/g, respectively. Isolates from both samples were identified to be Aspergillus flavus based on spore type and conidial structure of isolates. An increasing trend in the AFC of Cavendish bananas was observed during dried banana chips processing. Variability in the AFC between production batches was attributed to differences in aerobic and fungal populations and physicochemical characteristics of the fruits, peel damage of the raw materials, concentration of AFC in the air and food-contact surfaces of the production area, and temperature and relative humidity (RH) conditions of the environment during production and storage. Physicochemical characteristics of Cavendish bananas from the receipt of raw materials up to the first day of drying were within the reported range of values allowing growth and toxin production by aflatoxigenic fungi. Air-borne AFC varied depending on the section of the production area examined. The close proximity of the waste disposal area from the production operation to the preparation, drying and storage areas suggests that cross-contamination, probably air-borne or insect-borne was a likely occurrence. The hands of workers were also identified as AFC sources. Results of this study highlight the need for the development of strategies to control aflatoxigenic fungi and aflatoxin contamination in Philippine dried Cavendish bananas.

  9. Gas exchange rates, plant height, yield components, and productivity of upland rice as affected by plant regulators

    Directory of Open Access Journals (Sweden)

    Rita de Cássia Félix Alvarez

    2012-10-01

    Full Text Available The objective of this work was to evaluate gas exchange rates, plant height, yield components, and productivity of upland rice, as affected by type and application time of plant growth regulators. A randomized block design, in a 4x2 factorial arrangement, with four replicates was used. Treatments consisted of three growth regulators (mepiquat chloride, trinexapac-ethyl, and paclobutrazol, besides a control treatment applied at two different phenological stages: early tillering or panicle primordial differentiation. The experiment was performed under sprinkler-irrigated field conditions. Net CO2 assimilation, stomatal conductance, plant transpiration, and water-use efficiency were measured four times in Primavera upland rice cultivar, between booting and milky grain phenophases. Gas exchange rates were neither influenced by growth regulators nor by application time. There was, however, interaction between these factors on the other variables. Application of trinexapac-ethyl at both tillering and differentiation stages reduced plant height and negatively affected yield components and rice productivity. However, paclobutrazol and mepiquat chloride applied at tillering, reduced plant height without affecting rice yield. Mepiquat chloride acted as a growth stimulator when applied at the differentiation stage, and significantly increased plant height, panicle number, and grain yield of upland rice.

  10. Bioaugmentation of Hydrogenispora ethanolica LX-B affects hydrogen production through altering indigenous bacterial community structure.

    Science.gov (United States)

    Yang, Zhiman; Guo, Rongbo; Shi, Xiaoshuang; He, Shuai; Wang, Lin; Dai, Meng; Qiu, Yanling; Dang, Xiaoxiao

    2016-07-01

    Bioaugmentation can facilitate hydrogen production from complex organic substrates, but it still is unknown how indigenous microbial communities respond to the added bacteria. Here, using a Hydrogenispora ethanolica LX-B (named as LX-B) bioaugmentation experiments, the distribution of metabolites and the responses of indigenous bacterial communities were investigated via batch cultivation (BC) and repeated batch cultivation (RBC). In BC the LX-B/sludge ratio of 0.12 achieved substantial high hydrogen yield, which was over twice that of control. In RBC one-time bioaugmentation and repeated batch bioaugmentation of LX-B resulted in the hydrogen yield that was average 1.2-fold and 0.8-fold higher than that in control, respectively. This improved hydrogen production performance mainly benefited from a shift in composition of the indigenous bacterial community caused by LX-B bioaugmentation. The findings represented an important step in understanding the relationship between bioaugmentation, a shift in bacterial communities, and altered bioreactor performance.

  11. Factors affecting catalysis of copper corrosion products in NDMA formation from DMA in simulated premise plumbing.

    Science.gov (United States)

    Zhang, Hong; Andrews, Susan A

    2013-11-01

    This study investigated the effects of corrosion products of copper, a metal commonly employed in household plumbing systems, on N-nitrosodimethylamine (NDMA) formation from a known NDMA precursor, dimethylamine (DMA). Copper-catalyzed NDMA formation increased with increasing copper concentrations, DMA concentrations, alkalinity and hardness, but decreased with increasing natural organic matter (NOM) concentration. pH influenced the speciation of chloramine and the interactions of copper with DMA. The transformation of monochloramine (NH2Cl) to dichloramine and complexation of copper with DMA were involved in elevating the formation of NDMA by copper at pH 7.0. The inhibiting effect of NOM on copper catalysis was attributed to the rapid consumption of NH2Cl by NOM and/or the competitive complexation of NOM with copper to limit the formation of DMA-copper complexes. Hardness ions, as represented by Ca(2+), also competed with copper for binding sites on NOM, thereby weakening the inhibitory effect of NOM on NDMA formation. Common copper corrosion products also participated in these reactions but in different ways. Aqueous copper released from malachite [Cu2CO3(OH)2] was shown to promote NDMA formation while NDMA formation decreased in the presence of CuO, most likely due to the adsorption of DMA.

  12. Reproduction in the space environment: Part I. Animal reproductive studies

    Science.gov (United States)

    Santy, P. A.; Jennings, R. T.; Craigie, D.

    1990-01-01

    Mankind's exploration and colonization of the frontier of space will ultimately depend on men's and women's ability to live, work, and reproduce in the space environment. This paper reviews animal studies, from microorganisms to mammals, done in space or under space-simulated conditions, which identify some of the key areas which might interfere with human reproductive physiology and/or embryonic development. Those space environmental factors which impacted almost all species included: microgravity, artificial gravity, radiation, and closed life support systems. These factors may act independently and in combination to produce their effects. To date, there have been no studies which have looked at the entire process of reproduction in any animal species. This type of investigation will be critical in understanding and preventing the problems which will affect human reproduction. Part II will discuss these problems directly as they relate to human physiology.

  13. Organic loading rates affect composition of soil-derived bacterial communities during continuous, fermentative biohydrogen production

    Energy Technology Data Exchange (ETDEWEB)

    Luo, Yonghua; Bruns, Mary Ann [Department of Crop and Soil Sciences, The Pennsylvania State University, University Park, PA 16802 (United States); Zhang, Husen; Salerno, Michael; Logan, Bruce E. [Department of Civil and Environmental Engineering, The Pennsylvania State University, University Park, PA 16802 (United States)

    2008-11-15

    Bacterial community composition during steady-state, fermentative H{sub 2} production was compared across a range of organic loading rates (OLRs) of 0.5-19 g COD l{sup -1} h{sup -1} in a 2-l continuous flow reactor at 30 C. The varied OLRs were achieved with glucose concentrations of 2.5-10 g l{sup -1} and hydraulic retention times of 1-10 h. The synthetic wastewater feed was amended with L-cysteine and maintained at a pH of 5.5. For each run at a given glucose concentration, the reactor was inoculated with an aliquot of well-mixed agricultural topsoil that had been heat-treated to reduce numbers of vegetative cells. At OLRs less than 2 g COD l{sup -1} h{sup -1}, DNA sequences from ribosomal RNA intergenic spacer analysis profiles revealed more diverse and variable populations (Selenomonas, Enterobacter, and Clostridium spp.) than were observed above 2 g COD l{sup -1} h{sup -1} (Clostridium spp. only). An isolate, LYH1, was cultured from a reactor sample (10 g glucose l{sup -1} at a 10-h HRT) on medium containing L-cysteine. In confirming H{sub 2} production by LYH1 in liquid batch culture, lag periods for H{sub 2} production in the presence and absence of L-cysteine were 5 and 50 h, respectively. The 16S rRNA gene sequence of LYH1 indicated that the isolate was a Clostridium sp. affiliated with RNA subcluster Ic, with >99% similarity to Clostridium sp. FRB1. In fluorescent in situ hybridization tests, an oligonucleotide probe complementary to the 16S rRNA of LYH1 hybridized with 90% of cells observed at an OLR of 2 g COD h{sup -1}, compared to 26% of cells at an OLR of 0.5 g COD l{sup -1} h{sup -1}. An OLR of 2 g COD l{sup -1} h{sup -1} appeared to be a critical threshold above which clostridia were better able to outcompete Enterobacteriaceae and other organisms in the mixed soil inoculum. Our results are discussed in light of other biohydrogen studies employing pure cultures and mixed inocula. (author)

  14. UV-B affects photosynthesis, ROS production and motility of the freshwater flagellate, Euglena agilis Carter.

    Science.gov (United States)

    Kottuparambil, Sreejith; Shin, Woongghi; Brown, Murray T; Han, Taejun

    2012-10-15

    The effects of ultraviolet B (UV-B; 295-320 nm) radiation on certain vital physiological (photosynthesis), biochemical (production of reactive oxygen species - ROS) and behavioral (motility and orientation) characteristics were investigated in the unicellular photoautotroph, Euglena agilis Carter. The photosynthetic performance of E. agilis was recorded after exposure of between 15 and 60 min followed by a period of recovery lasting 6-24h under dim light (5-10 μmol photons m(-2) s(-1)). The maximum quantum yield of PS II (F(v)/F(m)) was reduced to 65% and 14% of initial values immediately following 15 and 30 min UV-B exposure, but recovered to 100 and 86% of the initials, respectively. Values of rETR(max) in E. agilis exposed to 15 min UV-B were similar to those of the initials, but a 30 min UV exposure resulted in 75% reduction of rETR(max) with only a 43% recovery as compared with the initial after 24h recovery. After a 60 min UV-B exposure, there were no Chl a fluorescence signals, and hence no F(v)/F(m) or rETR(max). A UV dose-dependent increase in DCFH-DA fluorescence was found in E. agilis cells, reflecting an increase in ROS production. After exposures to UV-B for between 15 and 60 min, the percentages of motile cells in the population decreased to 76, 39 and 15%, respectively. Following 24h in dim light, the percentage of motile cells increased to between 66% and 95% of the initial value. The velocity of non-irradiated cells was 60 μm s(-1), which decreased to 16-35 μm s(-1) immediately following exposure for 15-60 min. After periods of time in dim light (6, 12 and 24h) velocities had recovered to between 44 and 81% of the initial value. In untreated controls, the r-value was 0.23, indicating random movement of E. agilis, but it increased to 0.35 and 0.72 after exposure to UV-B for 30 and 60 min, respectively. There was a tendency towards vertical downward movement of cells proportional to the duration of exposure. The compactness of E. agilis decreased

  15. Farm labor, reproductive justice: Migrant women farmworkers in the US.

    Science.gov (United States)

    Galarneau, Charlene

    2013-06-12

    Little is known about the reproductive health of women migrant farmworkers in the US. The health and rights of these workers are advanced by fundamental human rights principles that are sometimes conceptually and operationally siloed into three approaches: reproductive health, reproductive rights, and reproductive justice. I focus on the latter framework, as it lends critical attention to the structural oppression central to poor reproductive health, as well as to the agency of communities organizing and leading efforts to improve their health. I review what is known about these women's reproductive health; identify three realms of reproduction oppression affecting their reproductive health: labor/occupational conditions, health care, and social relations involving race, immigration and fertility; and then highlight some current efforts at women farmworker-directed change. Finally, I make several analytical observations that suggest the importance of the reproductive justice framework to broader discussions of migrant worker justice and its role in realizing their right to health.

  16. Seedling quality of common sage (Salvia officinalis L. as affected by seedling production methods

    Directory of Open Access Journals (Sweden)

    Ömer Çalişkan

    2006-09-01

    Full Text Available The study was conducted to determine the effects of four different seedling production methods (i.e., open seedbed, greenhouse seedbed, float system and modified float system on the seedling growth and quality of common sage. Sowing rates were 1 g per m2 in an open seedbed and a greenhouse seedbed system, whereas the sowing rate was one seed per cell in the float and modified float systems. Emergence period, seedling growth period, the fresh and dry weights of aerial parts of seedlings, the fresh and dry weights of roots, seedling height, root height and stem diameter were determined as agronomical observations and measurements. The greenhouse seedling method was found to be superior over the other methods

  17. Factors Affecting the Production of Aromatic Immonium Ions in MALDI 157 nm Photodissociation Studies

    Science.gov (United States)

    DeGraan-Weber, Nick; Ashley, Daniel C.; Keijzer, Karlijn; Baik, Mu-Hyun; Reilly, James P.

    2016-05-01

    Immonium ions are commonly observed in the high energy fragmentation of peptide ions. In a MALDI-TOF/TOF mass spectrometer, singly charged peptides photofragmented with 157 nm VUV light yield a copious abundance of immonium ions, especially those from aromatic residues. However, their intensities may vary from one peptide to another. In this work, the effect of varying amino acid position, peptide length, and peptide composition on immonium ion yield is investigated. Internal immonium ions are found to have the strongest intensity, whereas immonium ions arising from C-terminal residues are the weakest. Peptide length and competition among residues also strongly influence the immonium ion production. Quantum calculations provide insights about immonium ion structures and the fragment ion conformations that promote or inhibit immonium ion formation.

  18. Small doses, big troubles: modeling growth dynamics of organisms affecting microalgal production cultures in closed photobioreactors.

    Science.gov (United States)

    Forehead, Hugh I; O'Kelly, Charles J

    2013-02-01

    The destruction of mass cultures of microalgae by biological contamination of culture medium is a pervasive and expensive problem, in industry and research. A mathematical model has been formulated that attempts to explain contaminant growth dynamics in closed photobioreactors (PBRs). The model simulates an initial growth phase without PBR dilution, followed by a production phase in which culture is intermittently removed. Contaminants can be introduced at any of these stages. The model shows how exponential growth from low initial inocula can lead to "explosive" growth in the population of contaminants, appearing days to weeks after inoculation. Principal influences are contaminant growth rate, PBR dilution rate, and the size of initial contaminant inoculum. Predictions corresponded closely with observed behavior of two contaminants, Uronema sp. and Neoparamoeba sp., found in operating PBRs. A simple, cheap and effective protocol was developed for short-term prediction of contamination in PBRs, using microscopy and archived samples.

  19. Factors affecting production of nonaqueous peracetic acid in tubular packed reactors

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The synthesis of nonaqueous peracetic acid in acetone by acetaldehyde oxidation was carried out in a tubular packed reactor.The influencing factors of the reacting system including packing material,oxygen carrier,and reactor configuration were investigated.The results show that porous materials are inappropriate for peracetic acid synthesis and only non porous material with appropriate surface area can provide good peracetic acid selectivity and yield.Among the six kinds of packing material investigated,SA-5118 is the best one.As oxidizing gas,pure oxygen is superior to air.The optimum length-to-inner diameter ratio of the reactor is about 40.Under the proper reaction conditions,the highest peracetic acid yield of 84.15% and the highest selectivity of 93.34% can be achieved which indicates that the novel reacting system is effective and economical for nonaqueous peracetic acid production.

  20. The factors affecting the green brand equity of electronic products: Green marketing

    Directory of Open Access Journals (Sweden)

    Narges Delafrooz

    2015-12-01

    Full Text Available This study is intentionally functional, and, from the data collection aspect, is a descriptive survey. The statistical data for the present study includes low-power electronic and electric products in Guilan Province. A total of 384 consumers were chosen using the random cluster sampling method. We have used confirmatory factor analysis and structural equation modeling to analyze the given data. The findings show that there is a significant relationship among brand-perceived quality, green brand image, (GBI and brand value, but not with the green brand-perceived value (GBPV. In addition, the results of the study show that brand credibility has a significant relationship with the GBPV and GBI, but not with the GBPV and GBI, each of which are associated with the green brand in a different way. At the end of this survey, you will read about the intermediate variables, all of which are acceptable, except the intermediate variables of brand credibility and GBI.

  1. Factors Affecting Embryogenic Callus Production and Plant Regeneration in Anther Culture of Bupleurum chinense

    Institute of Scientific and Technical Information of China (English)

    YANG Cheng-min; ZHAO Yu-kai; WEI Jian-he; ZHAO Li-zi; SUI Chun; ZHANG Zheng; CUI Lu-ying

    2011-01-01

    Objective To evaluate the influences of the genotypes,anther developmental stages,and cultural conditions on the efficiency of embryogenic callus induction and plant regeneration in the anthers culture of Bupleurum chinense.Methods The different effects such as four genotypes,plant growth regulators,and temperature condition were compared in the experiments.The histological study was performed with the process of the anther culture.Results The highest inducing rate of embryogenic calli were achieved for the genotypes Zhongcaiyihao(ZCYH),Z4,and Z5 at the early-to middle-uninucleate stages,except for genotype ZPM1 at the tetrad stage.Cold pretreatment increased the production of the embryogenic callus,in which 4-day cold pretreatment improved the production of embryogenic callus from 0% to 2.2% and 5.0% for genotypes ZPM1 and ZCYH,respectively.No embryogenic callus was induced in the medium containing less than 0.75 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D).The highest regeneration rate (34.6%)was obtained in 1/2 MS salts regeneration medium supplemented with 0.1 mg/L 6-benzylmaminopurine (BA).The low concentration of BA was able to promote the embryogenic callus formation and subsequent plantlet regeneration via somatic embryogenesis.Chromosome counting of regenerated plantlets showed mostly diploid plant (2n = 12)with only one haploid plant(n = 6).Because of the low rate of microspore embryo formation,we only tracked the process of embryogenesis from the connective tissue,instead of microspore by histological observations.Conclusion This study establishes an efficient system for embryogenic callus induction and plant regeneration system.This is the first report on the haploid plantlet through the anther culture orB.chinense.

  2. Disruption of quercetin metabolism by fungicide affects energy production in honey bees (Apis mellifera).

    Science.gov (United States)

    Mao, Wenfu; Schuler, Mary A; Berenbaum, May R

    2017-02-13

    Cytochrome P450 monooxygenases (P450) in the honey bee, Apis mellifera, detoxify phytochemicals in honey and pollen. The flavonol quercetin is found ubiquitously and abundantly in pollen and frequently at lower concentrations in honey. Worker jelly consumed during the first 3 d of larval development typically contains flavonols at very low levels, however. RNA-Seq analysis of gene expression in neonates reared for three days on diets with and without quercetin revealed that, in addition to up-regulating multiple detoxifying P450 genes, quercetin is a negative transcriptional regulator of mitochondrion-related nuclear genes and genes encoding subunits of complexes I, III, IV, and V in the oxidative phosphorylation pathway. Thus, a consequence of inefficient metabolism of this phytochemical may be compromised energy production. Several P450s metabolize quercetin in adult workers. Docking in silico of 121 pesticide contaminants of American hives into the active pocket of CYP9Q1, a broadly substrate-specific P450 with high quercetin-metabolizing activity, identified six triazole fungicides, all fungal P450 inhibitors, that dock in the catalytic site. In adults fed combinations of quercetin and the triazole myclobutanil, the expression of five of six mitochondrion-related nuclear genes was down-regulated. Midgut metabolism assays verified that adult bees consuming quercetin with myclobutanil metabolized less quercetin and produced less thoracic ATP, the energy source for flight muscles. Although fungicides lack acute toxicity, they may influence bee health by interfering with quercetin detoxification, thereby compromising mitochondrial regeneration and ATP production. Thus, agricultural use of triazole fungicides may put bees at risk of being unable to extract sufficient energy from their natural food.