Cardiac Dysfunction in a Porcine Model of Pediatric Malnutrition

DEFF Research Database (Denmark)

Fabiansen, Christian; Lykke, Mikkel; Hother, Anne-Louise

2015-01-01

BACKGROUND: Half a million children die annually of severe acute malnutrition and cardiac dysfunction may contribute to the mortality. However, cardiac function remains poorly examined in cases of severe acute malnutrition. OBJECTIVE: To determine malnutrition-induced echocardiographic disturbances...... and longitudinal changes in plasma pro-atrial natriuretic peptide and cardiac troponin-T in a pediatric porcine model. METHODS AND RESULTS: Five-week old piglets (Duroc-x-Danish Landrace-x-Yorkshire) were fed a nutritionally inadequate maize-flour diet to induce malnutrition (MAIZE, n = 12) or a reference diet...... groups. The myocardial performance index was 86% higher in MAIZE vs AGE-REF (pMalnutrition associates with cardiac dysfunction in a pediatric porcine model by increased myocardial performance index and pro-atrial natriuretic peptide...

Corneal epithelial cell viability of an ex vivo porcine eye model.

Science.gov (United States)

Chan, Ka Yin; Cho, Pauline; Boost, Maureen

2014-07-01

The aim was to assess the consistency of corneal epithelial cell viability of an ex vivo porcine eye model. Six porcine eye models (four test and two control) were prepared for each experiment. The model has a computer-controlled mechanical arm, which could move the eyelid of the porcine eye and apply phosphate buffered saline to simulate blinking and lacrimation. The four test eyes were set up to simulate evaporative dry eyes with simulated lacrimation and blinking (one blink and one drop of buffered saline per minute) over three hours. Control A models were set up to collect pre-experimental baseline data, while those of control B were the same as the test eyes but without lacrimation and blinking simulation. All porcine eyes were kept in a closed chamber with temperature and humidity well controlled. After three hours, the cells of all eyes (except control A, which were assessed immediately before commencement of the experiment) were assessed. The eyes were first dipped into 0.4 per cent trypan blue solution. Following the dissection and separation of the cells, the number of dead cells were then counted under the microscope with a field size of 0.25 mm(2). The experiment was repeated 11 times. No significant differences were found in the number of dead cells among the four test eyes in both the central and peripheral cornea. There were significantly more dead cells in the test eyes compared to control A but significantly less when compared to control B. More dead cells were found in the central cornea than the peripheral cornea in the test eyes but the difference was not observed in controls A and B. Epithelial cell viabilities among the four porcine eye models with simulated lacrimation and blinking were consistent. The majority of cells were viable before the experiment and simulated lacrimation and blinking maintained more viable cells over time. © 2014 The Authors. Clinical and Experimental Optometry © 2014 Optometrists Association Australia.

Characterisation of the porcine eyeball as an in-vitro model for dry eye.

Science.gov (United States)

Menduni, Francesco; Davies, Leon N; Madrid-Costa, D; Fratini, Antonio; Wolffsohn, James S

2018-02-01

To characterise the anatomical parameters of the porcine eye for potentially using it as a laboratory model of dry eye. Anterior chamber depth and angle, corneal curvature, shortest and longest diameter, endothelial cell density, and pachymetry were measured in sixty freshly enucleated porcine eyeballs. Corneal steepest meridian was 7.85±0.32mm, corneal flattest meridian was 8.28±0.32mm, shortest corneal diameter was 12.69±0.58mm, longest corneal diameter was 14.88±0.66mm and central corneal ultrasonic pachymetry was 1009±1μm. Anterior chamber angle was 28.83±4.16°, anterior chamber depth was 1.77±0.27mm, and central corneal thickness measured using OCT was 1248±144μm. Corneal endothelial cell density was 3250±172 cells/mm 2 . Combining different clinical techniques produced a pool of reproducible data on the porcine eye anatomy, which can be used by researchers to assess the viability of using the porcine eye as an in-vitro/ex-vivo model for dry eye. Due to the similar morphology with the human eye, porcine eyeballs may represent a useful and cost effective model to individually study important key factors in the development of dry eye, such as environmental and mechanical stresses. Copyright © 2017 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved.

Prevention of primary vascular graft infection with silver-coated polyester graft in a porcine model

DEFF Research Database (Denmark)

Gao, H; Sandermann, J; Prag, J

2010-01-01

To evaluate the efficacy of a silver-coated vascular polyester graft in the prevention of graft infection after inoculation with Staphylococcus aureus in a porcine model.......To evaluate the efficacy of a silver-coated vascular polyester graft in the prevention of graft infection after inoculation with Staphylococcus aureus in a porcine model....

Beskrivelse og modellering af produktionssystemer, Tulip

DEFF Research Database (Denmark)

Jacobsen, Peter

1997-01-01

produktionssystemer til brug ved optimering af drift og planlægning/udvikling af nye produktionssystemer. For at opnå et nødvendigt informationsgrundlag udføres projektet i samarbejde med udvalgte virksomheder.Rapporten er udført i samarbejde med Tulip International A/S i Vejle, hvor den del, der omfatter...

Evaluation of hands-on seminar for reduced port surgery using fresh porcine cadaver model

Directory of Open Access Journals (Sweden)

Saseem Poudel

2016-01-01

Full Text Available Background: The use of various biological and non-biological simulators is playing an important role in training modern surgeons with laparoscopic skills. However, there have been few reports of the use of a fresh porcine cadaver model for training in laparoscopic surgical skills. The purpose of this study was to report on a surgical training seminar on reduced port surgery using a fresh cadaver porcine model and to assess its feasibility and efficacy. Materials and Methods: The hands-on seminar had 10 fresh porcine cadaver models and two dry boxes. Each table was provided with a unique access port and devices used in reduced port surgery. Each group of 2 surgeons spent 30 min at each station, performing different tasks assisted by the instructor. The questionnaire survey was done immediately after the seminar and 8 months after the seminar. Results: All the tasks were completed as planned. Both instructors and participants were highly satisfied with the seminar. There was a concern about the time allocated for the seminar. In the post-seminar survey, the participants felt that the number of reduced port surgeries performed by them had increased. Conclusion: The fresh cadaver porcine model requires no special animal facility and can be used for training in laparoscopic procedures.

Evaluation of hands-on seminar for reduced port surgery using fresh porcine cadaver model.

Science.gov (United States)

Poudel, Saseem; Kurashima, Yo; Shichinohe, Toshiaki; Kitashiro, Shuji; Kanehira, Eiji; Hirano, Satoshi

2016-01-01

The use of various biological and non-biological simulators is playing an important role in training modern surgeons with laparoscopic skills. However, there have been few reports of the use of a fresh porcine cadaver model for training in laparoscopic surgical skills. The purpose of this study was to report on a surgical training seminar on reduced port surgery using a fresh cadaver porcine model and to assess its feasibility and efficacy. The hands-on seminar had 10 fresh porcine cadaver models and two dry boxes. Each table was provided with a unique access port and devices used in reduced port surgery. Each group of 2 surgeons spent 30 min at each station, performing different tasks assisted by the instructor. The questionnaire survey was done immediately after the seminar and 8 months after the seminar. All the tasks were completed as planned. Both instructors and participants were highly satisfied with the seminar. There was a concern about the time allocated for the seminar. In the post-seminar survey, the participants felt that the number of reduced port surgeries performed by them had increased. The fresh cadaver porcine model requires no special animal facility and can be used for training in laparoscopic procedures.

AF-6 Protects Against Dopaminergic Dysfunction and Mitochondrial Abnormalities in Drosophila Models of Parkinson’s Disease

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Adeline H. Basil

2017-08-01

Full Text Available Afadin 6 (AF-6 is an F-actin binding multidomain-containing scaffolding protein that is known for its function in cell-cell adhesion. Interestingly, besides this well documented role, we recently found that AF-6 is a Parkin-interacting protein that augments Parkin/PINK1-mediated mitophagy. Notably, mutations in Parkin and PINK1 are causative of recessively inherited forms of Parkinson’s disease (PD and aberrant mitochondrial homeostasis is thought to underlie PD pathogenesis. Given the novel role of AF-6 in mitochondrial quality control (QC, we hypothesized that AF-6 overexpression may be beneficial to PD. Using the Drosophila melanogaster as a model system, we demonstrate in this study that transgenic overexpression of human AF-6 in parkin and also pink1 null flies rescues their mitochondrial pathology and associated locomotion deficit, which results in their improved survival over time. Similarly, AF-6 overexpression also ameliorates the pathological phenotypes in flies expressing the Leucine Rich Repeat Kinase 2 (LRRK2 G2019S mutant, a mutation that is associated with dominantly-inherited PD cases in humans. Conversely, when endogenous AF-6 expression is silenced, it aggravates the disease phenotypes of LRRK2 mutant flies. Aside from these genetic models, we also found that AF-6 overexpression is protective against the loss of dopaminergic neurons in flies treated with rotenone, a mitochondrial complex I inhibitor commonly used to generate animal models of PD. Taken together, our results demonstrate that AF-6 protects against dopaminergic dysfunction and mitochondrial abnormalities in multiple Drosophila models of PD, and suggest the therapeutic value of AF-6-related pathways in mitigating PD pathogenesis.

Pancreas specific expression of oncogenes in a porcine model

DEFF Research Database (Denmark)

Berthelsen, Martin Fogtmann; Callesen, Morten Møbjerg; Østergaard, Tanja Stenshøj

2017-01-01

crucial for successful treatment. However, pancreatic cancer is difficult to detect in its earliest stages and once symptoms appear, the cancer has often progressed beyond possibility for curing. Research into the disease has been hampered by the lack of good models. We have generated a porcine m...

Udvikling af studerendes akademiske skrivekompetencer – en model for en indsats på fakultetsniveau

Directory of Open Access Journals (Sweden)

Tine Wirenfeldt Jensen

2017-03-01

Full Text Available I artiklen præsenteres en model for, hvordan den faglige ledelse på enkeltuddannelser og den faglige ledelse på tværs af flere uddannelser (her konkret et helt fakultet kan igangsætte arbejdet med udviklingen af de studerendes akademiske skrivekompetencer på en måde, der både er forankret i de enkelte faglige miljøer og samtidig muliggør opsamling af erfaringer og behov på tværs af fag. Modellen er udviklet og afprøvet i forbindelse med et projekt afviklet i efteråret 2015 i samarbejde med samtlige syv institutter på Aarhus BSS ved Aarhus Universitet. Særligt fremhæves tre af modellens styrker: 1. Modellen faciliterer, at flere centrale faglige aktører involveres i arbejdet med at understøtte de studerendes skrivekompetencer, 2. Modellen giver indsigt i studerendes aktuelle udfordringer og behov på tværs af et fakultet (her i form af 354 skriftlige evalueringer og 3. Modellen har vist potentiale til at motivere fagene til at fortsætte arbejdet med at understøtte de studerendes skrivekompetencer på egen hånd og tilføre nødvendig viden til dette fortsatte arbejde. This article presents a model for initiating development of academic writing skills in a manner that is both embedded in the disciplines and makes it possible to gain insight into students’ experiences and needs across departments. The model is developed and tested in the fall 2015 with all 7 departments at Aarhus BSS, Aarhus University. Three of the models strengths are emphasized: 1. The model facilitates the involvement of central players regarding the task of supporting students writing skills, 2. The model enables insight into the challenges and needs of student writers across faculty (the form of 354 written evaluations and 3. The model has shown potential to motivate departments to continue to focus on developing students’ writing skills.

Development of a Consistent and Reproducible Porcine Scald Burn Model

Science.gov (United States)

Kempf, Margit; Kimble, Roy; Cuttle, Leila

2016-01-01

There are very few porcine burn models that replicate scald injuries similar to those encountered by children. We have developed a robust porcine burn model capable of creating reproducible scald burns for a wide range of burn conditions. The study was conducted with juvenile Large White pigs, creating replicates of burn combinations; 50°C for 1, 2, 5 and 10 minutes and 60°C, 70°C, 80°C and 90°C for 5 seconds. Visual wound examination, biopsies and Laser Doppler Imaging were performed at 1, 24 hours and at 3 and 7 days post-burn. A consistent water temperature was maintained within the scald device for long durations (49.8 ± 0.1°C when set at 50°C). The macroscopic and histologic appearance was consistent between replicates of burn conditions. For 50°C water, 10 minute duration burns showed significantly deeper tissue injury than all shorter durations at 24 hours post-burn (p ≤ 0.0001), with damage seen to increase until day 3 post-burn. For 5 second duration burns, by day 7 post-burn the 80°C and 90°C scalds had damage detected significantly deeper in the tissue than the 70°C scalds (p ≤ 0.001). A reliable and safe model of porcine scald burn injury has been successfully developed. The novel apparatus with continually refreshed water improves consistency of scald creation for long exposure times. This model allows the pathophysiology of scald burn wound creation and progression to be examined. PMID:27612153

Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models.

Science.gov (United States)

Blutke, Andreas; Wanke, Rüdiger

2018-03-06

In translational medical research, porcine models have steadily become more popular. Considering the high value of individual animals, particularly of genetically modified pig models, and the often-limited number of available animals of these models, establishment of (biobank) collections of adequately processed tissue samples suited for a broad spectrum of subsequent analyses methods, including analyses not specified at the time point of sampling, represent meaningful approaches to take full advantage of the translational value of the model. With respect to the peculiarities of porcine anatomy, comprehensive guidelines have recently been established for standardized generation of representative, high-quality samples from different porcine organs and tissues. These guidelines are essential prerequisites for the reproducibility of results and their comparability between different studies and investigators. The recording of basic data, such as organ weights and volumes, the determination of the sampling locations and of the numbers of tissue samples to be generated, as well as their orientation, size, processing and trimming directions, are relevant factors determining the generalizability and usability of the specimen for molecular, qualitative, and quantitative morphological analyses. Here, an illustrative, practical, step-by-step demonstration of the most important techniques for generation of representative, multi-purpose biobank specimen from porcine tissues is presented. The methods described here include determination of organ/tissue volumes and densities, the application of a volume-weighted systematic random sampling procedure for parenchymal organs by point-counting, determination of the extent of tissue shrinkage related to histological embedding of samples, and generation of randomly oriented samples for quantitative stereological analyses, such as isotropic uniform random (IUR) sections generated by the "Orientator" and "Isector" methods, and vertical

Energioptimering af bæredygtighedscertificeringsprocesserne

DEFF Research Database (Denmark)

Christensen, Dorthe Holmberg; Carlsen, Mads; Alon, Shlomo Nevo

2014-01-01

Større fokus på bæredygtighedscertificeringer som DGNB har yderligere øget kompleksiteten af designet af bygninger. Introduktionen af teknologier som building information modelling (BIM) og proces metoder som integreret design proces (IDP) kan hjælpe med at reducere kompleksiteten og bedre...

Modellering af ATS forhold på Bjergmarken Renseanlæg

DEFF Research Database (Denmark)

Laursen, Jesper; Rasmussen, Michael R.

Denne rapport indeholder resultaterne af numeriske beregninger af en procestank opereret under ATS-drift på Bjergmarken Renseanlæg.......Denne rapport indeholder resultaterne af numeriske beregninger af en procestank opereret under ATS-drift på Bjergmarken Renseanlæg....

Monitering, modellering og miljøvurdering af fuldskala afværge af TCE fourening ved stimuleret reduktiv deklorering

DEFF Research Database (Denmark)

Westergaard, Claus; Lemming, Gitte; Janniche, Gry Sander

På Sortebrovej 26 i Tommerup blev der i 2006 gennemført afværgeforanstaltninger ved stimuleret reduktiv deklorering (SRD) ved injektion af donor og bakterier i kildeområdet med henblik på at oprense en forurening med TCE. Kildeområdet befinder sig 10-20 m u. t. i sandet og opsprækket moræneler...... gunstige forhold for nedbrydning af de klorerede ethener ved reduktiv deklorering. Der ses da også en klar dominans af nedbrydningsprodukterne DCE og VC samt ethen og ethan i grundvandet. I nogle boringer, ses dog en stagnerende omsætning af TCE og DCE i grundvandet. Årsagen her til kan være, at donoren...... da også fordoblet fra 2008 til 2010. Der er observeret zoner med betydelig omsætning af TCE, hvor cis-DCE dominerer, og der ses generelt VC og ethen centralt i disse zoner. Koncentrationsniveauet af fermenteringsprodukter er aftaget og består ved en sandslire (B370 K1) alene af acetat og methan...

Improved cell line IPEC-J2, characterized as a model for porcine jejunal epithelium.

Directory of Open Access Journals (Sweden)

Silke S Zakrzewski

Full Text Available Cell lines matching the source epithelium are indispensable for investigating porcine intestinal transport and barrier properties on a subcellular or molecular level and furthermore help to reduce animal usage. The porcine jejunal cell line IPEC-J2 is established as an in vitro model for porcine infection studies but exhibits atypically high transepithelial resistances (TER and only low active transport rates so that the effect of nutritional factors cannot be reliably investigated. This study aimed to properly remodel IPEC-J2 and then to re-characterize these cells regarding epithelial architecture, expression of barrier-relevant tight junction (TJ proteins, adequate TER and transport function, and reaction to secretagogues. For this, IPEC-J2 monolayers were cultured on permeable supports, either under conventional (fetal bovine serum, FBS or species-specific (porcine serum, PS conditions. Porcine jejunal mucosa was analyzed for comparison. Main results were that under PS conditions (IPEC-J2/PS, compared to conventional FBS culture (IPEC-J2/FBS, the cell height increased 6-fold while the cell diameter was reduced by 50%. The apical cell membrane of IPEC-J2/PS exhibited typical microvilli. Most importantly, PS caused a one order of magnitude reduction of TER and of trans- and paracellular resistance, and a 2-fold increase in secretory response to forskolin when compared to FBS condition. TJ ultrastructure and appearance of TJ proteins changed dramatically in IPEC-J2/PS. Most parameters measured under PS conditions were much closer to those of typical pig jejunocytes than ever reported since the cell line's initial establishment in 1989. In conclusion, IPEC-J2, if cultured under defined species-specific conditions, forms a suitable model for investigating porcine paracellular intestinal barrier function.

Anvendelse af MIP ved kildekarakterisering og vurdering af nedbrydning af chlorerede opløsningsmidler

DEFF Research Database (Denmark)

Broholm, Mette Martina; Janniche, Gry Sander; Damgaard, Ida

2014-01-01

Membrane Interface Probing (MIP) er et 'direct push' screeningsværktøj, som kan anvendes ved karakterisering af forurenede grunde. Karakterisering af kildeområder med chlorerede opløsningsmidler (som DNAPL) udgør et vigtigt led i udviklingen af den konceptuelle forståelse, som er essentiel...... for risikovurdering og valg af afværgestrategi. Integreret karakterisering med en række metoder for direkte såvel som indirekte dokumentation af DNAPL i moræneler, herunder MIP, er beskrevet i Kerrn-Jespersen et al. (2013). Det samlede DNAPL-karakteriseringsprojekt er beskrevet i detaljer i Janniche et al. (2013). En...... vurdering af anvendte detektorer for MIP er foretaget af Olsson (2013). Ved karakterisering af forurening med bl.a. chlorerede opløsningsmidler er der behov for en vurdering af forureningssammensætning og nedbrydning. Tilsvarende vurdering er nødvendig, når udviklingen af stimuleret reduktiv dechlorering...

Comparing the reported burn conditions for different severity burns in porcine models: a systematic review.

Science.gov (United States)

Andrews, Christine J; Cuttle, Leila

2017-12-01

There are many porcine burn models that create burns using different materials (e.g. metal, water) and different burn conditions (e.g. temperature and duration of exposure). This review aims to determine whether a pooled analysis of these studies can provide insight into the burn materials and conditions required to create burns of a specific severity. A systematic review of 42 porcine burn studies describing the depth of burn injury with histological evaluation is presented. Inclusion criteria included thermal burns, burns created with a novel method or material, histological evaluation within 7 days post-burn and method for depth of injury assessment specified. Conditions causing deep dermal scald burns compared to contact burns of equivalent severity were disparate, with lower temperatures and shorter durations reported for scald burns (83°C for 14 seconds) compared to contact burns (111°C for 23 seconds). A valuable archive of the different mechanisms and materials used for porcine burn models is presented to aid design and optimisation of future models. Significantly, this review demonstrates the effect of the mechanism of injury on burn severity and that caution is recommended when burn conditions established by porcine contact burn models are used by regulators to guide scald burn prevention strategies. © 2017 Medicalhelplines.com Inc and John Wiley & Sons Ltd.

Numerisk modellering af formfyldning ved støbning i selvkompakterende beton

DEFF Research Database (Denmark)

Spangenberg, Jon

dækker over mange emner. I denne afhandling er der fokuseret på tre af dem ved hjælp af Finite Difference Method (FDM) / Finite Volume Method (FVM) baserede Computational Fluid Dynamics (CFD) modeller udviklet i både FLOW-3D og MATLAB. Den første undersøgelse fokuserer på de komplikationer der er knyttet...... til at forudse separation under fyldning i SKB støbninger. De to FDM/FVM baserede CFD modeller afviger fra hinanden via deres tilslags repræsentation, som var en diskret metode (envejs momentum kobling) for den første og en skalar metode for den anden. Det viste sig, at det var mindst omfattende...

Porcine models of biofilm infections with focus on pathomorphology

DEFF Research Database (Denmark)

Jensen, Louise Kruse; Johansen, Anne Sofie Boyum; Jensen, Henrik Elvang

2017-01-01

, and reproducible animal models of the infections. In this review, the advantages of in vivo studies are compared to in vitro studies of biofilm formation in infectious diseases. The pig is the animal of choice when developing and applying large animal models of infectious diseases due to its similarity of anatomy......, physiology, and immune system to humans. Furthermore, conventional pigs spontaneously develop many of the same chronic bacterial infections as seen in humans. Therefore, in this review porcine models of five different infectious diseases all associated with biofilm formation and chronicity in humans...

Common data model for natural language processing based on two existing standard information models: CDA+GrAF.

Science.gov (United States)

Meystre, Stéphane M; Lee, Sanghoon; Jung, Chai Young; Chevrier, Raphaël D

2012-08-01

An increasing need for collaboration and resources sharing in the Natural Language Processing (NLP) research and development community motivates efforts to create and share a common data model and a common terminology for all information annotated and extracted from clinical text. We have combined two existing standards: the HL7 Clinical Document Architecture (CDA), and the ISO Graph Annotation Format (GrAF; in development), to develop such a data model entitled "CDA+GrAF". We experimented with several methods to combine these existing standards, and eventually selected a method wrapping separate CDA and GrAF parts in a common standoff annotation (i.e., separate from the annotated text) XML document. Two use cases, clinical document sections, and the 2010 i2b2/VA NLP Challenge (i.e., problems, tests, and treatments, with their assertions and relations), were used to create examples of such standoff annotation documents, and were successfully validated with the XML schemata provided with both standards. We developed a tool to automatically translate annotation documents from the 2010 i2b2/VA NLP Challenge format to GrAF, and automatically generated 50 annotation documents using this tool, all successfully validated. Finally, we adapted the XSL stylesheet provided with HL7 CDA to allow viewing annotation XML documents in a web browser, and plan to adapt existing tools for translating annotation documents between CDA+GrAF and the UIMA and GATE frameworks. This common data model may ease directly comparing NLP tools and applications, combining their output, transforming and "translating" annotations between different NLP applications, and eventually "plug-and-play" of different modules in NLP applications. Copyright © 2011 Elsevier Inc. All rights reserved.

Porcine model of hemophilia A.

Directory of Open Access Journals (Sweden)

Yuji Kashiwakura

Full Text Available Hemophilia A is a common X chromosome-linked genetic bleeding disorder caused by abnormalities in the coagulation factor VIII gene (F8. Hemophilia A patients suffer from a bleeding diathesis, such as life-threatening bleeding in the brain and harmful bleeding in joints and muscles. Because it could potentially be cured by gene therapy, subhuman animal models have been sought. Current mouse hemophilia A models generated by gene targeting of the F8 have difficulties to extrapolate human disease due to differences in the coagulation and immune systems between mice and humans. Here, we generated a porcine model of hemophilia A by nuclear transfer cloning from F8-targeted fibroblasts. The hemophilia A pigs showed a severe bleeding tendency upon birth, similar to human severe hemophiliacs, but in contrast to hemophilia A mice which rarely bleed under standard breed conditions. Infusion of human factor VIII was effective in stopping bleeding and reducing the bleeding frequency of a hemophilia A piglet but was blocked by the inhibitor against human factor VIII. These data suggest that the hemophilia A pig is a severe hemophilia A animal model for studying not only hemophilia A gene therapy but also the next generation recombinant coagulation factors, such as recombinant factor VIII variants with a slower clearance rate.

Lyden af musik - er lyden af idealisme

DEFF Research Database (Denmark)

Eigtved, Michael

2015-01-01

Programartiklen til Det Ny Teaters opsætning af Rodgers & Hammersteins The Sound of Music, undersøger musicalgenrens - og især de konkrete ophavsmænds- affinitet til fremstillingen af såvel idealistiske som ideologiske universer.......Programartiklen til Det Ny Teaters opsætning af Rodgers & Hammersteins The Sound of Music, undersøger musicalgenrens - og især de konkrete ophavsmænds- affinitet til fremstillingen af såvel idealistiske som ideologiske universer....

Quantification of Porcine Vocal Fold Geometry.

Science.gov (United States)

Stevens, Kimberly A; Thomson, Scott L; Jetté, Marie E; Thibeault, Susan L

2016-07-01

The aim of this study was to quantify porcine vocal fold medial surface geometry and three-dimensional geometric distortion induced by freezing the larynx, especially in the region of the vocal folds. The medial surface geometries of five excised porcine larynges were quantified and reported. Five porcine larynges were imaged in a micro-CT scanner, frozen, and rescanned. Segmentations and three-dimensional reconstructions were used to quantify and characterize geometric features. Comparisons were made with geometry data previously obtained using canine and human vocal folds as well as geometries of selected synthetic vocal fold models. Freezing induced an overall expansion of approximately 5% in the transverse plane and comparable levels of nonuniform distortion in sagittal and coronal planes. The medial surface of the porcine vocal folds was found to compare reasonably well with other geometries, although the compared geometries exhibited a notable discrepancy with one set of published human female vocal fold geometry. Porcine vocal folds are qualitatively geometrically similar to data available for canine and human vocal folds, as well as commonly used models. Freezing of tissue in the larynx causes distortion of around 5%. The data can provide direction in estimating uncertainty due to bulk distortion of tissue caused by freezing, as well as quantitative geometric data that can be directly used in developing vocal fold models. Copyright © 2016 The Voice Foundation. Published by Elsevier Inc. All rights reserved.

Surgical induction of choroidal neovascularization in a porcine model

DEFF Research Database (Denmark)

Lassota, Nathan; Kiilgaard, Jens Folke; Prause, Jan Ulrik

2007-01-01

PURPOSE: To develop a reproducible surgical technique for the induction of choroidal neovascularization (CNV) in the subretinal space of porcine eyes and to analyse the resulting CNV clinically and histologically. METHODS: Two different modifications of a surgical technique previously described...... were compared with the original method. In ten porcine eyes retinal pigment epithelial (RPE) cells were removed using a silicone tipped cannula, in ten porcine eyes Bruch's membrane was perforated once with a retinal perforator without prior RPE removal and in ten eyes RPE removal was followed...... by a single perforation of Bruch's membrane. Fifteen of the eyes, five from each group, were enucleated 30 minutes after surgery, while the remaining eyes were enucleated after 14 days. Prior to enucleation, at day 14, fundus photographs and fluorescein angiograms were obtained. Eyes were examined by light...

En genlæsning af John Rawls i lyset af hans diskussion af forholdet til fremtidige generationer

DEFF Research Database (Denmark)

Lübcke, Poul

2009-01-01

På grundlag af en konsekventialistisk rekonstruktion af Rawls' politiske position gøres der rede for Rawls eksplicitte og implicitte vurdering af vores ansvar over for fremtidige generationer......På grundlag af en konsekventialistisk rekonstruktion af Rawls' politiske position gøres der rede for Rawls eksplicitte og implicitte vurdering af vores ansvar over for fremtidige generationer...

Digital innovation af folkeskolernes åbenhed og sammenlignelighed

DEFF Research Database (Denmark)

Andersen, Kim Normann; Henriksen, Helle Zinner; Agger Nielsen, Jeppe

2013-01-01

Denne artikel præsenterer en normativ model (Public Sector Process Rebuilding, PPR), som kan indfange, hvor innovativt offentlige organisationer anvender digitale løsninger. Optag af digital innovation anskues ofte i litteraturen binært – enten er innovationen anskaffet eller ikke anskaffet. Vores...... argument er imidlertid, at optag af digitale innovationer kan gradbøjes. Vores sigte er derfor at få fokus væk fra anskaffelse og mere i retning af udnyttelse af digitaliseringen med henblik på at skabe forbedring af kerneydelserne og gavne slutbrugerne. Med empirisk afsæt i danske folkeskolers tolkning af...

Characterization of a porcine model of chronic superficial varicose veins.

Science.gov (United States)

Jones, Gregory T; Grant, Mark W; Thomson, Ian A; Hill, B Geraldine; van Rij, André M

2009-06-01

Previous animal models of venous disease, while inducing venous hypertension and valvular insufficiency, do not produce superficial varicose veins. In this study, we aimed to develop and characterize a pig-based model of superficial varicose veins. Right femoral arteriovenous fistulae (AVF) were surgically fashioned in young adult pigs. Animals were examined at postoperative times up to 15 weeks to determine the development of varicose veins and measurement of both blood pressure and flow velocities within the superficial thigh veins. Histology and vascular corrosion casts were used to characterize the resulting structural venous alterations. Porcine pathophysiological features were compared with those of human primary superficial varicose veins. Gross superficial varicosities developed over the ipsilateral medial thigh region after an initial lag period of 1-2 weeks. Veins demonstrated retrograde filling with valvular incompetence, and a moderate, non-pulsatile, venous hypertension, which was altered by changes in posture and Valsalva. Venous blood flow velocities were elevated to 15-30 cm/s in varicose veins. Structurally, pig varicose veins were enlarged, tortuous, had valvular degeneration, and regions of focal medial atrophy with or without overlying intimal thickening. The superficial varicose veins, which developed within this model, have a pathophysiology that is consistent with that observed in humans. The porcine femoral AVF model is proposed as a suitable experimental model to evaluate the pathobiology of superficial venous disease. It may also be suitable for the evaluation of treatment interventions including drug therapy.

Energirenovering af parcelhuse

DEFF Research Database (Denmark)

Aggerholm, Søren

af de tre huse består i efterisolering af lofter op til 300 mm, udskiftning af almindelige termoruder til energiruder, og installation af solvarmeanlæg til produktion af varmt brugsvand. I de tre huse er der ved energirenoveringen opnået en reduktion af energiforbruget på 16-22 procent. Rapporten...

Kortlægning af evalueringer og undersøgelser af vejledning knyttet til vejledning af unge i UU-regi

DEFF Research Database (Denmark)

Skovhus, Randi Boelskifte; thomsen, rie

2013-01-01

Kortlægning af evalueringer og undersøgelser af vejledning knyttet til vejledning af unge i UU-regi 2004-2013. Et arbejdspapir......Kortlægning af evalueringer og undersøgelser af vejledning knyttet til vejledning af unge i UU-regi 2004-2013. Et arbejdspapir...

Fremtidssikring af kloaknet

DEFF Research Database (Denmark)

Hansen, Ernst Jan de Place

2008-01-01

Beskrivelse af hvordan man ved udbygning af eksisterende kloaknet og ved nytænkning af planlægning af nye bydele kan sikre sig langsigtet mod konsekvenserne af klimaændinger og kraftigere nedbør...

Anatomical manifestations of primary blast ocular trauma observed in a postmortem porcine model.

Science.gov (United States)

Sherwood, Daniel; Sponsel, William E; Lund, Brian J; Gray, Walt; Watson, Richard; Groth, Sylvia L; Thoe, Kimberly; Glickman, Randolph D; Reilly, Matthew A

2014-02-24

We qualitatively describe the anatomic features of primary blast ocular injury observed using a postmortem porcine eye model. Porcine eyes were exposed to various levels of blast energy to determine the optimal conditions for future testing. We studied 53 enucleated porcine eyes: 13 controls and 40 exposed to a range of primary blast energy levels. Eyes were preassessed with B-scan and ultrasound biomicroscopy (UBM) ultrasonography, photographed, mounted in gelatin within acrylic orbits, and monitored with high-speed videography during blast-tube impulse exposure. Postimpact photography, ultrasonography, and histopathology were performed, and ocular damage was assessed. Evidence for primary blast injury was obtained. While some of the same damage was observed in the control eyes, the incidence and severity of this damage in exposed eyes increased with impulse and peak pressure, suggesting that primary blast exacerbated these injuries. Common findings included angle recession, internal scleral delamination, cyclodialysis, peripheral chorioretinal detachments, and radial peripapillary retinal detachments. No full-thickness openings of the eyewall were observed in any of the eyes tested. Scleral damage demonstrated the strongest associative tendency for increasing likelihood of injury with increased overpressure. These data provide evidence that primary blast alone (in the absence of particle impact) can produce clinically relevant ocular damage in a postmortem model. The blast parameters derived from this study are being used currently in an in vivo model. We also propose a new Cumulative Injury Score indicating the clinical relevance of observed injuries.

A porcine ex vivo lung perfusion model with maximal argon exposure to attenuate ischemia-reperfusion injury

Directory of Open Access Journals (Sweden)

An Martens

2017-01-01

Full Text Available Argon (Ar is a noble gas with known organoprotective effects in rodents and in vitro models. In a previous study we failed to find a postconditioning effect of Ar during ex vivo lung perfusion (EVLP on warm-ischemic injury in a porcine model. In this study, we further investigated a prolonged exposure to Ar to decrease cold ischemia-reperfusion injury after lung transplantation in a porcine model with EVLP assessment. Domestic pigs (n = 6/group were pre-conditioned for 6 hours with 21% O 2 and 79% N 2 (CONTR or 79% Ar (ARG. Subsequently, lungs were cold flushed and stored inflated on ice for 18 hours inflated with the same gas mixtures. Next, lungs were perfused for 4 hours on EVLP (acellular while ventilated with 12% O 2 and 88% N 2 (CONTR group or 88% Ar (ARG group. The perfusate was saturated with the same gas mixture but with the addition of CO 2 to an end-tidal CO 2 of 35-45 mmHg. The saturated perfusate was drained and lungs were perfused with whole blood for an additional 2 hours on EVLP. Evaluation at the end of EVLP did not show significant effects on physiologic parameters by prolonged exposure to Ar. Also wet-to-dry weight ratio did not improve in the ARG group. Although in other organ systems protective effects of Ar have been shown, we did not detect beneficial effects of a high concentration of Ar on cold pulmonary ischemia-reperfusion injury in a porcine lung model after prolonged exposure to Ar in this porcine model with EVLP assessment.

Stilladsering af undersøgende samtale og modellering

DEFF Research Database (Denmark)

Nielsen, Birgitte Lund; Brandt, Harald

med målet at udvikle og rammesætte elever og lærere som producenter af augmented reality (AR) ifm. naturfagsundervisning. AR handler kort fortalt om at udvide og lægge ekstra lag af fx billede og lyd på virkeligheden, som fx i Pokemon-go. I naturfag kan det handle om at “se ind i” fænomener, der er...

Spilscenarier i undervisningen - præsentation af en didaktisk model

DEFF Research Database (Denmark)

Hanghøj, Thorkild

2012-01-01

I takt med den stigende interesse for brugen af spil på videregående uddannelser er der behov for at udvikle tilsvarende didaktisk tænkning, som kan hjælpe undervisere med at integrere spil i deres undervisning. I denne artikel præsenteres en didaktisk model, der antager at spilbaseret undervisning...

Open wedge high tibial osteotomy using three-dimensional printed models: Experimental analysis using porcine bone.

Science.gov (United States)

Kwun, Jun-Dae; Kim, Hee-June; Park, Jaeyoung; Park, Il-Hyung; Kyung, Hee-Soo

2017-01-01

The purpose of this study was to evaluate the usefulness of three-dimensional (3D) printed models for open wedge high tibial osteotomy (HTO) in porcine bone. Computed tomography (CT) images were obtained from 10 porcine knees and 3D imaging was planned using the 3D-Slicer program. The osteotomy line was drawn from the three centimeters below the medial tibial plateau to the proximal end of the fibular head. Then the osteotomy gap was opened until the mechanical axis line was 62.5% from the medial border along the width of the tibial plateau, maintaining the posterior tibial slope angle. The wedge-shaped 3D-printed model was designed with the measured angle and osteotomy section and was produced by the 3D printer. The open wedge HTO surgery was reproduced in porcine bone using the 3D-printed model and the osteotomy site was fixed with a plate. Accuracy of osteotomy and posterior tibial slope was evaluated after the osteotomy. The mean mechanical axis line on the tibial plateau was 61.8±1.5% from the medial tibia. There was no statistically significant difference (P=0.160). The planned and post-osteotomy correction wedge angles were 11.5±3.2° and 11.4±3.3°, and the posterior tibial slope angle was 11.2±2.2° pre-osteotomy and 11.4±2.5° post-osteotomy. There were no significant differences (P=0.854 and P=0.429, respectively). This study showed that good results could be obtained in high tibial osteotomy by using 3D printed models of porcine legs. Copyright © 2016 Elsevier B.V. All rights reserved.

Digital visualisering i udbygning af Roskilde Universitetscenter

DEFF Research Database (Denmark)

Sørensen, Nils Lykke

Rapporten beskriver tre forsøg med 3D-visualisering i forbindelse med konkurrencer vedrørende udbygning af Roskilde Universitetscenter. Første forsøg var en metode til hurtigt at skabe en række digitale modeller i højt abstraktionsniveau. Forsøget resulterede i 15 små animationer, der blev benyttet...... af dommerkomiteen til volumenstudier af bygningskonkurrencens indsendte forslag. Andet forsøg var afprøvning af et modelleringsarbejde i lavere abstraktionsniveau, som resulterede i en animation af vinderprojektet. I sidste forsøg blev de praktiske erfaringer fra de to første forsøg implementeret hos...

Fermentering af korn øger fordøjeligheden af næringsstoffer

DEFF Research Database (Denmark)

Pedersen, Anni Øyan; Jørgensen, Henry Johs. Høgh; Knudsen, Knud Erik Bach

2010-01-01

Et fordøjelighedsforsøg har vist, at energiværdien af byg og hvede steg ved fermentering. Effekten var størst ved fermentering af byg, hvor fordøjeligheden af organisk stof for enden af tyndtarmen steg statistisk sikkert med 9 %. Ved fermentering af hvede steg fordøjeligheden af organisk stof...... af vand og dels CO2-produktion og dermed tørstoftab. Anbefaling om beregning af energiværdi i fermenteret korn vil blive foretaget ud fra resultaterne af et produktionsforsøg med slagtesvin, som er under publicering. Fosforudnyttelse i fermenteret korn undersøges yderligere i et produktionsforsøg med...

Model Predictions and Measured Skin Damage Thresholds for 1.54 Micrometers Laser Pulses in Porcine Skin

National Research Council Canada - National Science Library

Roach, William P; Cain, Clarence; Schuster, Kurt; Stockton, Kevin; Stolarski, David S; Galloway, Robert; Rockwell, Benjamin

2004-01-01

A new source-term thermal model was used to determine the skin temperature rise using porcine skin parameters for various wavelengths, pulse durations, and laser spot sizes and is compared to the Takata thermal model...

Udvikling af strategiske alliancer, joint ventures og netværk

DEFF Research Database (Denmark)

Gustafsson, Jeppe; Henriksen, Lars Bo; Larsson, Rune

Rapporten er et resultat af et projekt om virksomhedssamarbejde under Industri- og Handelsstyrelsens program "Ledelse af samarbejde om teknologisk fornyelse". Formålet med projektet har været at bidrage til udvikling af anvendelsesorienterede modeller vedrørende udvikling og ledelse af alliancer ...

Lyden-af-Leg

DEFF Research Database (Denmark)

Toft, Herdis

Præsentation af seniorforsker-projekt Lyden-af-Leg i et traderingsperspektiv og med indledende fokus på YouTube som traderings-platform.......Præsentation af seniorforsker-projekt Lyden-af-Leg i et traderingsperspektiv og med indledende fokus på YouTube som traderings-platform....

Sundhedseffekter af luftforurening-beregningspriser

DEFF Research Database (Denmark)

Andersen, M. S.; Frohn, L. M.; Jensen, S. S.

Denne rapport afrapporterer det fortsatte arbejde med udvikling af miljøøkonomiske beregningspriser. På grundlag af EcoSense 4.0 fra ExternE-projektet, er der foretaget nye beregninger af eksternaliteter ved luftforurening med sundhedseffekter. Samtidig giver rapporten en vurdering af luftspredni......Denne rapport afrapporterer det fortsatte arbejde med udvikling af miljøøkonomiske beregningspriser. På grundlag af EcoSense 4.0 fra ExternE-projektet, er der foretaget nye beregninger af eksternaliteter ved luftforurening med sundhedseffekter. Samtidig giver rapporten en vurdering af...

Anvendelse af alternative isoleringsmaterialer

DEFF Research Database (Denmark)

Hansen, Ernst Jan de Place

2003-01-01

Resume af By og Byg Anvisning 207 om anvendelse af alternative isoleringsmaterialer, udarbejdet af Statens Byggeforskningsinstitut under udviklingsprogrammet "Miljø- og arbejdsmiljøvenlig isolering"......Resume af By og Byg Anvisning 207 om anvendelse af alternative isoleringsmaterialer, udarbejdet af Statens Byggeforskningsinstitut under udviklingsprogrammet "Miljø- og arbejdsmiljøvenlig isolering"...

Professionel udvikling af naturfagslærere

DEFF Research Database (Denmark)

Krogh, Lars Brian

2016-01-01

& Hollingsworth, 2002) som model for tænkning omkring TPD, samt anvende en mange-lags evalueringsoptik i overensstemmelse med Guskey (2002). Den eksisterende viden om, hvad der skal til for at professionelle udviklingsaktiviteter får virkning opsummeres som afsæt for en drøftelse af TPD-design. Endelig...

Beregning af nitratudvaskning

DEFF Research Database (Denmark)

Petersen, Jens; Petersen, Bjørn Molt; Blicher-Mathiesen, Gitte

2006-01-01

I forbindelse med kommunalreformen ønskes en ensartet forvaltning af reglerne for vurdering af virkninger på miljøet (VVM) ved ansøgninger om udvidelse af husdyrbrug, herunder beregning af nitratudvaskningen. Rapporten foreslår et beregningskoncept, der bygger på den ansøgende bedrifts samlede kv...

Identifikation af referencevandløb til implementering af vandrammedirektivet i Danmark

DEFF Research Database (Denmark)

Kristensen, Esben Astrup; Baattrup-Pedersen, Annette; Skriver, Jens

Definition og beskrivelse af referencetilstanden er centralt for implementering af vandrammedirektivet, da bedømmelse af den økologiske tilstand sker som afvigelsen fra den upåvirkede referencetilstand. I denne rapport præsenteres analyser af danske, udenlandske og historiske data med det formål...

Restaurering af Skjern Å

DEFF Research Database (Denmark)

Jessen, K.; Boysen Larsen, B.; Bundgaard, P.

Denne rapport indeholder resultaterne af den natur- og miljøovervågning, der blev iværksat som en del af naturgenopretningen af Skjern Å 1999-2002. Ved projektet ophørte den kunstige afvanding af den vestlige del af Skjern Å dalen. Størstedelen af de dyrkede arealer ændredes til ekstensive græsni...

Tema 1: Programmering af robotenheder i grundskolen

Directory of Open Access Journals (Sweden)

Stine Ejsing-Duun

2016-01-01

Full Text Available I denne artikel undersøger vi potentialerne for at lære matematik gennem programmering af robotter. Vi foreslår en model med tre indbyrdes afhængige læringspotentialer, som programmering fordrer. Det omfatter elevens evne til at; (1 tænke i algoritmer, (2 producere viden og artefakter gennem brug af matematik og (3 foretage abstraktion og indkapsling. Programmering er blevet en del af grundskolens læringsmål i flere lande. I Danmark er programmering et læringsmål i forenklede fælles mål for Fysik/Kemi og desuden en del af den nationale undervisningsvejledning for matematik. En analyse af potentialerne i at anvende programmering i forbindelse med disse fag er derfor væsentlig. I denne artikel fokuserer vi på samspillet mellem matematik og programmering gennem litteraturstudier og analyse af empiriske situationer fra undervisning i programmering af LEGO Mindstorms. Teoretisk anvendes den instrumentelle tilgang til teknologi i matematikundervisning. Analysen viser en række måder, hvorpå didaktisk opmærksomhed på epistemisk forhandling kan understøtte læring af matematik gennem programmering.

Relevansen af Aristoteles' etik for konceptionen af eksistentiel terapi

DEFF Research Database (Denmark)

Dræby, Anders

Siden sin undfangelse i begyndelsen af det 20. århundrede har den eksistentielle terapi repræsenteret et filosofisk funderet alternativ til de former for terapeutisk praksis, der er funderet i den funktionalistiske medicinske metafysik. Anvendelse af elementer fra Aristoteles' etik muliggør en...... fremhævelse af den eksistentielle terapi som en praktisk eksistensfænomenologisk livskunst, hvis anliggende er en frembringelse af det enkelte menneskes muligheder fra det skjulte og ind i det uskjulte, så mennesket viser sig som det er ved sig selv...

Om distributionen af hvidhed

DEFF Research Database (Denmark)

Frello, Birgitta

2007-01-01

Med udgangspunkt i John Fords Western, Forfølgeren fra 1956, præsenteres en analyse af repræsentationer af race og overskridelse af raceskel, særligt i relation til konstitueringen af subjektpositioner, hvorfra spørgsmål om hvidhed og ikke-hvidhed kan afgøres.......Med udgangspunkt i John Fords Western, Forfølgeren fra 1956, præsenteres en analyse af repræsentationer af race og overskridelse af raceskel, særligt i relation til konstitueringen af subjektpositioner, hvorfra spørgsmål om hvidhed og ikke-hvidhed kan afgøres....

Energirenovering af Traneparken

DEFF Research Database (Denmark)

Rose, Jørgen; Thomsen, Kirsten Engelund; Mørck, Ove

Nærværende rapport udgør en del af afrapporteringen af PSO ELFORSK projektet Vejledning for energirenovering af boligblokke til lavenergiklasse 2015 og 2020. Arbejdet har været finansieret af PSO midler via ELFORSK, projekt: 347-023. Formålet med projektet er at udvikle en vejledning for dybtgående...

Methods for the detection and serum depletion of porcine galectin-3.

Science.gov (United States)

Eliaz, Isaac; Patil, Aarti; Navarro-Alvarez, Nalu; Wang, Zhirui; Eliaz, Amity; Weil, Elaine; Wilk, Barry; Sachs, David H; Huang, Christene A

2017-10-01

Circulating galectin-3 (Gal-3) is elevated in systemic inflammatory disorders, fibrotic diseases, and in cancers. Gal-3 is a promising cancer target where it promotes tumorigenesis and metastasis, as well as in renal, pulmonary, hepatic, and cardiovascular diseases, because of its role as a driver of fibrotic remodeling. This reports goal was to establish methods for the detection and removal of porcine Gal-3 that will enable further studies of the therapeutic potential of Gal-3 depletion by apheresis in porcine disease models. The long-term aim is to develop a safe, effective method of removing Gal-3 via apheresis as a standalone therapeutic tool and as an adjuvant to other therapies. Purified recombinant porcine Gal-3 was prepared and used as the standard for development of a porcine Gal-3 enzyme-linked immunosorbent assay (ELISA). Different affinity column matrices that incorporated either a rat IgG2a anti-Gal-3 monoclonal antibody or carbohydrate ligand were assessed for depletion of Gal-3 from porcine serum. A porcine Gal-3 ELISA with a linear range from 0.3 to 20 ng/mL was able to detect native porcine Gal-3 in both fetal (∼150-200 ng/mL) and juvenile (∼5-15 ng/mL) porcine serum samples. Use of an anti-Gal-3 monoclonal antibody affinity column depleted Gal-3 from porcine serum to at least 313 pg/mL, the limit of ELISA detection. Methods have been developed for the detection and depletion of porcine Gal-3. These methods will be used to study the specific effects of Gal-3 depletion via apheresis in porcine models of disease. © 2017 Wiley Periodicals, Inc.

Induced Hypothermia Does Not Harm Hemodynamics after Polytrauma: A Porcine Model

Directory of Open Access Journals (Sweden)

Matthias Weuster

2015-01-01

Full Text Available Background. The deterioration of hemodynamics instantly endangers the patients’ life after polytrauma. As accidental hypothermia frequently occurs in polytrauma, therapeutic hypothermia still displays an ambivalent role as the impact on the cardiopulmonary function is not yet fully understood. Methods. We have previously established a porcine polytrauma model including blunt chest trauma, penetrating abdominal trauma, and hemorrhagic shock. Therapeutic hypothermia (34°C was induced for 3 hours. We documented cardiovascular parameters and basic respiratory parameters. Pigs were euthanized after 15.5 hours. Results. Our polytrauma porcine model displayed sufficient trauma impact. Resuscitation showed adequate restoration of hemodynamics. Induced hypothermia had neither harmful nor major positive effects on the animals’ hemodynamics. Though heart rate significantly decreased and mixed venous oxygen saturation significantly increased during therapeutic hypothermia. Mean arterial blood pressure, central venous pressure, pulmonary arterial pressure, and wedge pressure showed no significant differences comparing normothermic trauma and hypothermic trauma pigs during hypothermia. Conclusions. Induced hypothermia after polytrauma is feasible. No major harmful effects on hemodynamics were observed. Therapeutic hypothermia revealed hints for tissue protective impact. But the chosen length for therapeutic hypothermia was too short. Nevertheless, therapeutic hypothermia might be a useful tool for intensive care after polytrauma. Future studies should extend therapeutic hypothermia.

Induced Hypothermia Does Not Harm Hemodynamics after Polytrauma: A Porcine Model

Science.gov (United States)

Mommsen, Philipp; Pfeifer, Roman; Mohr, Juliane; Ruchholtz, Steffen; Flohé, Sascha; Fröhlich, Matthias; Keibl, Claudia; Seekamp, Andreas; Witte, Ingo

2015-01-01

Background. The deterioration of hemodynamics instantly endangers the patients' life after polytrauma. As accidental hypothermia frequently occurs in polytrauma, therapeutic hypothermia still displays an ambivalent role as the impact on the cardiopulmonary function is not yet fully understood. Methods. We have previously established a porcine polytrauma model including blunt chest trauma, penetrating abdominal trauma, and hemorrhagic shock. Therapeutic hypothermia (34°C) was induced for 3 hours. We documented cardiovascular parameters and basic respiratory parameters. Pigs were euthanized after 15.5 hours. Results. Our polytrauma porcine model displayed sufficient trauma impact. Resuscitation showed adequate restoration of hemodynamics. Induced hypothermia had neither harmful nor major positive effects on the animals' hemodynamics. Though heart rate significantly decreased and mixed venous oxygen saturation significantly increased during therapeutic hypothermia. Mean arterial blood pressure, central venous pressure, pulmonary arterial pressure, and wedge pressure showed no significant differences comparing normothermic trauma and hypothermic trauma pigs during hypothermia. Conclusions. Induced hypothermia after polytrauma is feasible. No major harmful effects on hemodynamics were observed. Therapeutic hypothermia revealed hints for tissue protective impact. But the chosen length for therapeutic hypothermia was too short. Nevertheless, therapeutic hypothermia might be a useful tool for intensive care after polytrauma. Future studies should extend therapeutic hypothermia. PMID:26170533

Establishment and characterization of a differentiated epithelial cell culture model derived from the porcine cervix uteri.

Science.gov (United States)

Miessen, Katrin; Einspanier, Ralf; Schoen, Jennifer

2012-03-19

Cervical uterine epithelial cells maintain a physiological and pathogen-free milieu in the female mammalian reproductive tract and are involved in sperm-epithelium interaction. Easily accessible, differentiated model systems of the cervical epithelium are not yet available to elucidate the underlying molecular mechanisms within these highly specialized cells. Therefore, the aim of the study was to establish a cell culture of the porcine cervical epithelium representing in vivo-like properties of the tissue. We tested different isolation methods and culture conditions and validated purity of the cultured cells by immunohistochemistry against keratins. We could reproducibly culture pure epithelial cells from cervical tissue explants. Based on a morphology score and the WST-1 Proliferation Assay, we optimized the growth medium composition. Primary porcine cervical cells performed best in conditioned Ham's F-12, containing 10% FCS, EGF and insulin. After cultivation in an air-liquid interface for three weeks, the cells showed a discontinuously multilayered phenotype. Finally, differentiation was validated via immunohistochemistry against beta catenin. Mucopolysaccharide production could be shown via alcian blue staining. We provide the first suitable protocol to establish a differentiated porcine epithelial model of the cervix uteri, based on easily accessible cells using slaughterhouse material.

Om Hegels opfattelse af Gorgias, set på baggrund af tidligere filosofihistorikeres fremstilling

DEFF Research Database (Denmark)

Huggler, Jørgen

2010-01-01

En undersøgelse af Hegels tolkning af sofisten Gorgias giver anledning til at se på receptionen af sofisterne hos notable filosofihistorikere i det 18. århundrede. En sådan undersøgelse viser, at Hegel har en original og velvillig tolkning af sofisterne koncentreret omkring begrebet Bildung og det...... at give grunde for noget. Hegels udlægning af Gorgias bygger på hans tolkning af eleaterne, især af Zenon. Når Hegel har en særlig tolkning af eleaterne, skyldes det hans egne mangeårige filosofiske bestræbelser, og en identificering af en særlig, immanent objektiv dialektik hos Zenon, som han finder......, at Gorgias fører videre. Hegels filosofiske forståelse er tilstrækkelig udviklet til at kunne knytte positive kommentarer til alle dele af diskussionen i Gorgias' ræsonnement Om det ikkeværende, eller Om naturen. Det lykkes dog bedst i forhold til de to første af Gorgias' tre underteser. I hegelsk perspektiv...

Nicotine permeability across the buccal TR146 cell culture model and porcine buccal mucosa in vitro

DEFF Research Database (Denmark)

Nielsen, Hanne Mørck; Rassing, Margrethe Rømer

2002-01-01

The present study was conducted to investigate and compare the effect of pH and drug concentration on nicotine permeability across the TR146 cell culture model and porcine buccal mucosa in vitro. As a further characterization of the TR146 cell culture model, it was explored whether the results were...... comparable for bi-directional and uni-directional transport in the presence of a transmembrane pH gradient. Nicotine concentrations between 10(-5) and 10(-2) M were applied to the apical side of the TR146 cell culture model or the mucosal side of porcine buccal mucosa. Buffers with pH values of 5.5, 7.......4 and 8.1 were used to obtain different fractions of non- and mono-ionized nicotine. The apparent permeability (P(app)) of nicotine across both models increased significantly with increasing pH, and the P(app) values obtained with the two models could be correlated in a linear manner. With increasing...

Ensretning af historieundervisningen

DEFF Research Database (Denmark)

Rønhede, Søren

2008-01-01

"Det er ikke kun i Putins Rusland, at statsmagten vil dirigere historieundervisningen." (Politiken havde fokuseret på "ensretning af historieundervisningen" i Rusland) "Kanonen fra 2006 er undfanget af Udvalget til Styrkelse af Historie i Folkeskolen. Dette udvalg havde regeringen nedsat; men bid...... bidrager kanonen til faglig styrkelse?" spørges der. Det vises, at flere kanonpunkter ikke er fagligt funderede, men af mytologisk art....

Deciphering the porcine intestinal microRNA transcriptome

Directory of Open Access Journals (Sweden)

Keller Andreas

2010-04-01

Full Text Available Abstract Background While more than 700 microRNAs (miRNAs are known in human, a comparably low number has been identified in swine. Because of the close phylogenetic distance to humans, pigs serve as a suitable model for studying e.g. intestinal development or disease. Recent studies indicate that miRNAs are key regulators of intestinal development and their aberrant expression leads to intestinal malignancy. Results Here, we present the identification of hundreds of apparently novel miRNAs in the porcine intestine. MiRNAs were first identified by means of deep sequencing followed by miRNA precursor prediction using the miRDeep algorithm as well as searching for conserved miRNAs. Second, the porcine miRNAome along the entire intestine (duodenum, proximal and distal jejunum, ileum, ascending and transverse colon was unraveled using customized miRNA microarrays based on the identified sequences as well as known porcine and human ones. In total, the expression of 332 intestinal miRNAs was discovered, of which 201 represented assumed novel porcine miRNAs. The identified hairpin forming precursors were in part organized in genomic clusters, and most of the precursors were located on chromosomes 3 and 1, respectively. Hierarchical clustering of the expression data revealed subsets of miRNAs that are specific to distinct parts of the intestine pointing to their impact on cellular signaling networks. Conclusions In this study, we have applied a straight forward approach to decipher the porcine intestinal miRNAome for the first time in mammals using a piglet model. The high number of identified novel miRNAs in the porcine intestine points out their crucial role in intestinal function as shown by pathway analysis. On the other hand, the reported miRNAs may share orthologs in other mammals such as human still to be discovered.

Bloodless laparoscopic liver resection using radiofrequency thermal energy in the porcine model.

Science.gov (United States)

Tsalis, Konstantinos; Blouhos, Konstantinos; Vasiliadis, Konstantinos; Kalfadis, Stavros; Tsachalis, Theodoros; Savvas, Ioannis; Betsis, Dimitrios

2007-02-01

The aim of this study was to assess the feasibility and safety of laparoscopic hepatectomy using radiofrequency (RF) thermal energy in a porcine model. Fifteen female domestic pigs weighing 29.3 kg (range 25 to 35 kg) were used. Five transversal abdominal incisions (3 of 1 cm and 2 of 0.5 cm) were made for the introduction of the video camera and the other laparoscopic instruments. With the porta hepatis not clamped, the liver was inspected and the preferred lobe each time was divided using RF (cool-tip electrode 3 cm) with minimum bleeding. Serum liver enzymes and blood counts were drawn pre and postoperatively. All animals were killed after 1 week. The mean time of the procedures was 119 minutes (range 100 to 155 min). There were no intraoperative complications. Mean blood loss was 27 mL (range 5 to 60 mL), and the mass of the resected specimen was 132.5 g (range 65 to 305 g). There were no postoperative complications or deaths. Bloodless laparoscopic hepatectomy was technically feasible and safe in the porcine model using cool-tip electrode and 500-kHz RF Generator.

Sequence conservation between porcine and human LRRK2

DEFF Research Database (Denmark)

Larsen, Knud; Madsen, Lone Bruhn

2009-01-01

 Leucine-rich repeat kinase 2 (LRRK2) is a member of the ROCO protein superfamily (Ras of complex proteins (Roc) with a C-terminal Roc domain). Mutations in the LRRK2 gene lead to autosomal dominant Parkinsonism. We have cloned the porcine LRRK2 cDNA in an attempt to characterize conserved...... and expression patterns are conserved across species. The porcine LRRK2 gene was mapped to chromosome 5q25. The results obtained suggest that the LRRK2 gene might be of particular interest in our attempt to generate a transgenic porcine model for Parkinson's disease...

Håndteringen af musikfiler i krydsfeltet mellem downloading og streaming

DEFF Research Database (Denmark)

Ægidius, Andreas Lenander

remedieringen af musikfilen som stream ud fra den konceptuelle model for et kulturkredsløb, hvori et kulturelt artefakts betydning dannes (Gay mfl., 2013). Kommodificeringer og rekommodificering af musikfiler og håndteringer bliver herved sammenkædet med remediering og konvergens (Bolter & Grusin, 2000; Jenkins...... kommodificerer streamrip med fokus på at gøre filkonverteringer lette at anvende. Sidstnævnte viser jeg med analyse af brugen af software, der ripper musik fra YouTube. Jeg finder modstridende artikulationer af lydkvalitet blandt de tre grupper. Formatet og repræsentationen i diverse platformes brugerflader er...... digitale online musikbrug. Et konkret eksempel herpå er det sammensurium af transkodninger, som følger af YouTubes repræsentation, regulering, produktion af stream og lytternes efterfølgende brug, herunder streamrip. Dette sammensurium af transkodninger betegner jeg som en fonografisk effekt af YouTube...

Fastlæggelse af oprensningskriterier for grundvandstruende forureninger

DEFF Research Database (Denmark)

Døssing Overheu, Niels; Tuxen, Nina; Thomsen, Nanna Isbak

med numerisk model vil være for omkostningskrævende. På baggrund af bl.a. en litteraturgennemgang af Troldborg (2010) er der udvalgt 5 modeller (JAGG1.5, REMChlor, RISC4, ConSim og DTUV1D), som er afprøvet på 5 forurenede lokaliteter, der bredt repræsenterer den danske geologi og hydrogeologi, og...

Styring af kommunal digitalisering

DEFF Research Database (Denmark)

Jæger, Birgit

2017-01-01

Digitalisering af både interne arbejdsprocesser og kommunikation med borgere og virksomheder har længe været en vigtig faktor i moderniseringen af den offentlige sektor. Derfor er der stort fokus på styring af digitaliseringen. Denne artikel undersøger, dels hvordan digitaliseringen i den kommunale...... sektor styres, og dels hvilke styringsparadigmer der kan identificeres. Med udgangspunkt i en konkret kommune analyseres styringen af digitalisering gennem et hierarki af offentlige digitaliseringsstrategier. Gennem udvikling af en helt ny form for organisering, en såkaldt Digitaliseringsforening...

Livscyklusvurdering af basiskemikalier

DEFF Research Database (Denmark)

Olsen, Stig Irving

1998-01-01

Dette resumé af metoder og væsentlige erfaringer fra erhvervsforskerprojektet Livscyklusvurdering af basiskemikalier er skrevet i erkendelse af, at hovedrapporten er for omfattende til en hurtig introduktion i problemstillingerne. Formålet med denne rapport er derfor at give et kort overblik over...

Globalisering af uddannelse

DEFF Research Database (Denmark)

Jensen, Henning Høgh

2005-01-01

Det globale marked for uddannelse er i voldsom vækst og er ved at blive et af Danmarks vigtigste vareområder. Hvorledes kan KVL agere i globaliseringen af uddannelse for at sikre en høj kvalitet af egne kandidater med gode jobmuligheder?...

Test af et satellitbaseret kørselsafgiftssystem

DEFF Research Database (Denmark)

Sørensen, Simon Bojer; Lahrmann, Harry

2007-01-01

I dette paper beskrives opbygningen af en model af et takstsystem, som skal fungere i et satellitbaseret kørselsafgiftssystem. Modellen er forholdsvis simpel og bygger på et buffersystem, og der anvendes således ikke nogen avanceret map matching. Det beskrives, hvordan modellen afprøves med tilfæ...

Undervisning af tosprogede elever

DEFF Research Database (Denmark)

Horst, Christian

2003-01-01

Artiklen fremdrager hovedresultaterne fra Virginia P. Collier's og Wayne P. Thomas's længdeundersøgelser af tosprogede elever i USA, som formentlig er de mest omfattende undersøgelser af undervisningen af tosprogede elever overhovedet. Resultaterne diskuteres i relation til udviklingen af en...

Progress, problems and prospects of porcine pluripotent stem cells

Directory of Open Access Journals (Sweden)

Hanning WANG,Yangli PEI,Ning LI,Jianyong HAN

2014-02-01

Full Text Available Pluripotent stem cells (PSCs, including embryonic stem cells (ESCs and induced PSCs (iPSCs, can differentiate into cells of the three germ layers, suggesting that PSCs have great potential for basic developmental biology research and wide applications for clinical medicine. Genuine ESCs and iPSCs have been derived from mice and rats, but not from livestock such as the pig─an ideal animal model for studying human disease and regenerative medicine due to similarities with human physiologic processes. Efforts to derive porcine ESCs and iPSCs have not yielded high-quality PSCs that can produce chimeras with germline transmission. Thus, exploration of the unique porcine gene regulation network of preimplantation embryonic development may permit optimization of in vitro culture systems for raising porcine PSCs. Here we summarize the recent progress in porcine PSC generation as well as the problems encountered during this progress and we depict prospects for generating porcine naive PSCs.

Risk Factor Analyses for the Return of Spontaneous Circulation in the Asphyxiation Cardiac Arrest Porcine Model

Directory of Open Access Journals (Sweden)

Cai-Jun Wu

2015-01-01

Full Text Available Background: Animal models of asphyxiation cardiac arrest (ACA are frequently used in basic research to mirror the clinical course of cardiac arrest (CA. The rates of the return of spontaneous circulation (ROSC in ACA animal models are lower than those from studies that have utilized ventricular fibrillation (VF animal models. The purpose of this study was to characterize the factors associated with the ROSC in the ACA porcine model. Methods: Forty-eight healthy miniature pigs underwent endotracheal tube clamping to induce CA. Once induced, CA was maintained untreated for a period of 8 min. Two minutes following the initiation of cardiopulmonary resuscitation (CPR, defibrillation was attempted until ROSC was achieved or the animal died. To assess the factors associated with ROSC in this CA model, logistic regression analyses were performed to analyze gender, the time of preparation, the amplitude spectrum area (AMSA from the beginning of CPR and the pH at the beginning of CPR. A receiver-operating characteristic (ROC curve was used to evaluate the predictive value of AMSA for ROSC. Results: ROSC was only 52.1% successful in this ACA porcine model. The multivariate logistic regression analyses revealed that ROSC significantly depended on the time of preparation, AMSA at the beginning of CPR and pH at the beginning of CPR. The area under the ROC curve in for AMSA at the beginning of CPR was 0.878 successful in predicting ROSC (95% confidence intervals: 0.773∼0.983, and the optimum cut-off value was 15.62 (specificity 95.7% and sensitivity 80.0%. Conclusions: The time of preparation, AMSA and the pH at the beginning of CPR were associated with ROSC in this ACA porcine model. AMSA also predicted the likelihood of ROSC in this ACA animal model.

Establishment and characterization of a differentiated epithelial cell culture model derived from the porcine cervix uteri

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Miessen Katrin

2012-03-01

Full Text Available Abstract Background Cervical uterine epithelial cells maintain a physiological and pathogen-free milieu in the female mammalian reproductive tract and are involved in sperm-epithelium interaction. Easily accessible, differentiated model systems of the cervical epithelium are not yet available to elucidate the underlying molecular mechanisms within these highly specialized cells. Therefore, the aim of the study was to establish a cell culture of the porcine cervical epithelium representing in vivo-like properties of the tissue. Results We tested different isolation methods and culture conditions and validated purity of the cultured cells by immunohistochemistry against keratins. We could reproducibly culture pure epithelial cells from cervical tissue explants. Based on a morphology score and the WST-1 Proliferation Assay, we optimized the growth medium composition. Primary porcine cervical cells performed best in conditioned Ham's F-12, containing 10% FCS, EGF and insulin. After cultivation in an air-liquid interface for three weeks, the cells showed a discontinuously multilayered phenotype. Finally, differentiation was validated via immunohistochemistry against beta catenin. Mucopolysaccharide production could be shown via alcian blue staining. Conclusions We provide the first suitable protocol to establish a differentiated porcine epithelial model of the cervix uteri, based on easily accessible cells using slaughterhouse material.

Description and evaluation of a bench porcine model for teaching surgical residents vascular anastomosis skills

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Jauch Karl-Walter

2010-07-01

Full Text Available Abstract Background Numerous models, of variable quality, exist to impart the complex skills required to perform vascular anastomosis. These models differ with regard to the kinds of materials used, as well as their sizes, the time needed for their preparation, their availability, and the associated costs. The present study describes a bench model that uses formalin-fixed porcine aorta, and its evaluation by young surgical residents during a recent skills course. Findings The aortic segments used were a by-product of slaughtering. They were fixed and stored after harvesting for eventual use. Ten young surgical residents participated, and each performed one end-to-side vascular anastomosis. The evaluation was a questionnaire maintaining anonymity of the participant containing questions addressing particular aspects of the model and the experiences of the trainee, along with their ratings concerning the need for a training course to learn vascular anastomosis techniques. The scoring on the survey was done using a global 6-point rating scale (Likert Scale. In addition, we ranked the present model by reviewing the current literature for models that address vascular anastomosis skills. The trainees who participated were within their first two years of training (1.25 ± 0.46. A strong agreement in terms of the necessity of training for vascular anastomosis techniques was evident among the participating trainees (5.90 ± 0.32, who had only few prior manual experiences (total number 1.50 ± 0.53. The query revealed a strong agreement that porcine aorta is a suitable model that fits the needs for training vascular anastomosis skills (5.70 ± 0.48. Only a few bench models designed to teach surgical residents vascular anastomosis techniques were available in the literature. Conclusions The preparatory and financial resources needed to perform anastomosis skills training using porcine aorta are few. The presented bench model appears to be appropriate for

6 belysninger af vejledning

DEFF Research Database (Denmark)

Toft, Helle Mary; Jessing, Carla Tønder; Nymann, Anette

Artiklerne belyser vejledning af unge og vejledning af voksne. I relation til unge drejer det sig om: 1. vejledning af unge med vanskeligheder i forhold til uddannelse - her unge med særlige vejledningsbehov, og mentorordninger for unge, og 2. vejledning af unge i ungdomsuddannelser og i erhvervs...

Brug af alternativ isolering i forbindelse med renovering af ældre etageejendomme

DEFF Research Database (Denmark)

Hansen, Ernst Jan de Place

2003-01-01

Resume af rapport om praktiske erfaringer med brug af alternative isoleringsmaterialer i en renoveringsopgave, udarbejdet af Byfornyelse København m.fl. under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"...

Preliminary study of selenium and mercury distribution in some porcine tissues and their subcellular fractions by NAA and HG-AFS

International Nuclear Information System (INIS)

Jiujiang Zhao; Chunying Chen; Peiqun Zhang; Zhifang Chai

2004-01-01

Selenium and mercury distribution in porcine tissues and their subcellular fractions from a mercury-polluted area of Guizhou Province and from a not mercury-exposed area of Beijing in China have been studied with neutron activation analysis and hydride generation-atomic fluorescence spectrometry. Both the selenium and mercury levels are higher in Guizhou porcine tissues and their subcellular fractions than those in Beijing. These two elements are highly enriched in kidney and liver of Guizhou pig, while selenium is only enriched in the kidney of Beijing pig. Exposure of mercury may result in redistribution of Se and Hg in vivo. The Hg/Se molar ratio of the subcellular fractions is very low in the case of relatively low mercury level and gradually reaches to a high constant value with increasing level of mercury, which implies that selenium and mercury may form some special complexes in the organisms. (author)

Behandling af elmesyge

DEFF Research Database (Denmark)

Thomsen, Iben Margrete; Kristoffersen, Palle

2004-01-01

Ingen midler er p.t. godkendt og dermed lovlige til bekæmpelse af elmesyge i Danmark. Lovprisning af nye behandlinger ses f.eks. på internettet, men dokumentation mangler. Økonomien og nytten ved en kemisk bekæmpelse frem for sanering af syge træer skal afvejes nøje....

Udvikling af materialer til affaldsforbrænding – Nye materialer til overlagssvejsning

DEFF Research Database (Denmark)

Tiedje, Niels Skat

Denne del af projektet havde til formål at udvikle en metode til hurtig benchmarking af nye legeringer til overlagssvejsning og at anvende metoden i kombination med termodynamisk modellering af mikrostrukturer i svejste Ni–baserede legeringer. På baggrund af disse analyser skulle der udvikles nye...

Energirenovering af Sems Have

DEFF Research Database (Denmark)

Jensen, Søren Østergaard; Rose, Jørgen; Mørck, Ove

Nærværende rapport udgør en del af afrapporteringen af PSO ELFORSK projektet Vejledning for energirenovering af boligblokke til lavenergiklasse 2015 og 2020. Arbejdet har været finansieret af PSO midler via ELFORSK, projekt: 347-023. Formålet med projektet er at udvikle en vejledning for dybtgående...

Midler til lindring af mundtørhed og stimulation af spytsekretion

DEFF Research Database (Denmark)

Dynesen, Anja Weirsøe; Pedersen, Anne Marie Lynge; Jensen, Allan Bardow

2013-01-01

effekt. I relation til risikoen for at udvikle caries, der er forhøjet hos den mundtørre patient, er det dog væsentligt at anbefale produkter uden sukker. Ligesom produktets indhold af syre og dermed det erosive potentiale er af betydning i forhold til udviklingen af dentale erosioner. Systemisk...

Af Upraktiske Grunde

DEFF Research Database (Denmark)

Vaaben, Nana Katrine

2001-01-01

som både Claude Levi-Strauss lægger op til i sine klassiske studier af "den vilde tanke", og som Jean Baudrillard siden har lagt op til i sine analyser af samlere som en slags ekstreme forbrugere, der er i færd med at komplettere deres identitet gennem besiddelser af genstande. Imidlertid tillægger...

Ledelse af projektmyldret

DEFF Research Database (Denmark)

større arbejdsgrupper, og det kan være selvorganiserede store udviklingsprogrammer. Et projekt kører sjældent alene, og derfor er der ikke kun tale om projektledelse, men i lige så høj grad om ledelse af projekter. Ledelse af virksomhedens mylder af projekter er ikke let, og forfatterne gennemgår derfor...

Evaluering af ordning med psykologbehandling af personer med let til moderat depression

DEFF Research Database (Denmark)

Fjeldsted, Rita; Christensen, Kaj Sparle

Herværende rapport evaluerer den pr. 1.4.2008 indførte ordning med mulighed for henvisning til psykologbehandling med 60 % tilskud for patienter med let til moderat depression. Evalueringen har betjent sig af flere gensidigt supplerende metoder: spørgeskemaundersøgelser overfor patienter, psykolo......Herværende rapport evaluerer den pr. 1.4.2008 indførte ordning med mulighed for henvisning til psykologbehandling med 60 % tilskud for patienter med let til moderat depression. Evalueringen har betjent sig af flere gensidigt supplerende metoder: spørgeskemaundersøgelser overfor patienter...... tidligere evaluering af forsøgsordning af psykologbehandling af depression i perioden 2005-06. 18.419 personer i alderen 18-37 år blev henvist til psykolog i den undersøgte ordning i perioden 1.2.2009 – 31.1.2010, svarende til en henvisningshyppighed på 1,6 % af baggrundsbefolkningen i samme aldersgruppe....... Ordningen giver mulighed for én rehenvisning, og 9 % blev rehenvist i perioden. Spørgeskemaresultater viser, at kun 33 % af de henviste falder ind under henvisningskriteriet ” let til moderat depression” på tidspunktet for opstart af psykologbehandling, 39 % har ingen tegn på depression, og 28 % har svær...

Studerendes oplevelse af reorganisering af problembaseret læring på Aalborg Universitet

Directory of Open Access Journals (Sweden)

Bettina Dahl

2016-04-01

Full Text Available På Aalborg Universitet skete der i 2010 en omorganisering af stu-dieordningerne på Det Teknisk-Naturvidenskabelige Fakultet. Blandt ændringerne var, at de traditionelle projekter fyldte mindre, en større adskillelse af den formelle sammenhæng mellem kurser og projekter og en opdeling af kurserne i blokke på 5 ECTS. Vi sammenlignede erfaringerne med både den nye og den gamle model hos 10.-semesterstuderende på tre uddannelser og konkluderede, at de adspurgte studerende lagde stor vægt på projektarbejdet både i den gamle og den nye model, men at der var en signifikant lavere oplevelse af sammenhæng på et semester nu ift. før. De studerende prioriterede nu kursuseksamen højere til sidst i semesteret. Overordnet viste undersøgelsen også, at forholdet mellem det fagorienterede og det problemorienterede i de to PBL-modeller, og PBL-modeller generelt, ofte ikke var som modellerne overordnet gav udtryk for. Eksempelvis havde den nye model flere fagorienterede kurser og var derfor fra én synsvinkel et skridt mod en mere fagorienteret PBL-model; på den anden side havde de studerende i den nye model tidsmæssigt stadig mest fokus på det problemorienterede projekt og de fagorienterede kurser indeholdt PBL-lignende miniprojekter. De studerendes adfærd i en ændret undervisningsmodel var således mere kompleks, end man umiddelbart kunne antage. In 2010 Aalborg University reorganized the curriculum at the Faculty of Engineering and Science. The new curriculum scheduled less time for group projects and there was a formal separation between project and course work in each semester. The courses were also divided into 5 ECTS blocks. We compared student experiences of the old and new curriculums for three study programmes. Our results reveal that whereas in the old curriculum, students would prioritize the projects, with the new curriculum there was more focus on course work and linked examinations and the students found each semester more

Studerendes oplevelse af reorganisering af problembaseret læring på Aalborg Universitet

Directory of Open Access Journals (Sweden)

Bettina Dahl

2015-08-01

Full Text Available På Aalborg Universitet skete der i 2010 en omorganisering af stu-dieordningerne på Det Teknisk-Naturvidenskabelige Fakultet. Blandt ændringerne var, at de traditionelle projekter fyldte mindre, en større adskillelse af den formelle sammenhæng mellem kurser og projekter og en opdeling af kurserne i blokke på 5 ECTS. Vi sammenlignede erfaringerne med både den nye og den gamle model hos 10.-semesterstuderende på tre uddannelser og konkluderede, at de adspurgte studerende lagde stor vægt på projektarbejdet både i den gamle og den nye model, men at der var en signifikant lavere oplevelse af sammenhæng på et semester nu ift. før. De studerende prioriterede nu kursuseksamen højere til sidst i semesteret. Overordnet viste undersøgelsen også, at forholdet mellem det fagorienterede og det problemorienterede i de to PBL-modeller, og PBL-modeller generelt, ofte ikke var som modellerne overordnet gav udtryk for. Eksempelvis havde den nye model flere fagorienterede kurser og var derfor fra én synsvinkel et skridt mod en mere fagorienteret PBL-model; på den anden side havde de studerende i den nye model tidsmæssigt stadig mest fokus på det problemorienterede projekt og de fagorienterede kurser indeholdt PBL-lignende miniprojekter. De studerendes adfærd i en ændret undervisningsmodel var således mere kompleks, end man umiddelbart kunne antage. In 2010 Aalborg University reorganized the curriculum at the Faculty of Engineering and Science. The new curriculum scheduled less time for group projects and there was a formal separation between project and course work in each semester. The courses were also divided into 5 ECTS blocks. We compared student experiences of the old and new curriculums for three study programmes. Our results reveal that whereas in the old curriculum, students would prioritize the projects, with the new curriculum there was more focus on course work and linked examinations and the students found each semester more

Snit af målinger: VK afhængig af DF og NA

DEFF Research Database (Denmark)

Thomsen, Søren Risbjerg

2007-01-01

ANALYSE: Ekstra Risbjerg-snit af meningsmålinger grundet fortsat valgrummel - VK-regeringen vil blive afhængig af både DF og NA. Udgivelsesdato: 21. august 2007......ANALYSE: Ekstra Risbjerg-snit af meningsmålinger grundet fortsat valgrummel - VK-regeringen vil blive afhængig af både DF og NA. Udgivelsesdato: 21. august 2007...

Lentiviral Vector Gene Transfer to Porcine Airways

Directory of Open Access Journals (Sweden)

Patrick L Sinn

2012-01-01

Full Text Available In this study, we investigated lentiviral vector development and transduction efficiencies in well-differentiated primary cultures of pig airway epithelia (PAE and wild-type pigs in vivo. We noted gene transfer efficiencies similar to that observed for human airway epithelia (HAE. Interestingly, feline immunodeficiency virus (FIV-based vectors transduced immortalized pig cells as well as pig primary cells more efficiently than HIV-1–based vectors. PAE express TRIM5α, a well-characterized species-specific lentiviral restriction factor. We contrasted the restrictive properties of porcine TRIM5α against FIV- and HIV-based vectors using gain and loss of function approaches. We observed no effect on HIV-1 or FIV conferred transgene expression in response to porcine TRIM5α overexpression or knockdown. To evaluate the ability of GP64-FIV to transduce porcine airways in vivo, we delivered vector expressing mCherry to the tracheal lobe of the lung and the ethmoid sinus of 4-week-old pigs. One week later, epithelial cells expressing mCherry were readily detected. Our findings indicate that pseudotyped FIV vectors confer similar tropisms in porcine epithelia as observed in human HAE and provide further support for the selection of GP64 as an appropriate envelope pseudotype for future preclinical gene therapy studies in the porcine model of cystic fibrosis (CF.

Comparison of commercial and experimental porcine circovirus type 2 (PCV2) vaccines using a triple challenge with PCV2, porcine reproductive and respiratory syndrome virus (PRRSV), and porcine parvovirus (PPV).

Science.gov (United States)

Shen, H G; Beach, N M; Huang, Y W; Halbur, P G; Meng, X J; Opriessnig, T

2010-08-23

The efficacies of commercial porcine circovirus type 2 (PCV2) vaccines and a live PCV1-2a chimeric vaccine were compared in conventional, PCV2-positive piglets using a PCV2-porcine reproductive and respiratory syndrome virus (PRRSV)-porcine parvovirus (PPV) coinfection challenge model. Seventy-three, 2-week-old pigs were randomized into seven groups including five vaccinated and two control groups. Pigs in the vaccinated groups were vaccinated at 3 weeks (one dose) or at 3 and 6 weeks (two dose) of age. All vaccine regimens tested were effective in reducing naturally occurring PCV2 viremia at 16 weeks of age and after PCV2 challenge, demonstrating the capability of the products to induce a lasting protective immunity despite the presence of PCV2 viremia at the time of vaccination. Copyright 2010 Elsevier Ltd. All rights reserved.

A Triple Culture Model of the Blood-Brain Barrier Using Porcine Brain Endothelial cells, Astrocytes and Pericytes.

Science.gov (United States)

Thomsen, Louiza Bohn; Burkhart, Annette; Moos, Torben

2015-01-01

In vitro blood-brain barrier (BBB) models based on primary brain endothelial cells (BECs) cultured as monoculture or in co-culture with primary astrocytes and pericytes are useful for studying many properties of the BBB. The BECs retain their expression of tight junction proteins and efflux transporters leading to high trans-endothelial electric resistance (TEER) and low passive paracellular permeability. The BECs, astrocytes and pericytes are often isolated from small rodents. Larger species as cows and pigs however, reveal a higher yield, are readily available and have a closer resemblance to humans, which make them favorable high-throughput sources for cellular isolation. The aim of the present study has been to determine if the preferable combination of purely porcine cells isolated from the 6 months old domestic pigs, i.e. porcine brain endothelial cells (PBECs) in co-culture with porcine astrocytes and pericytes, would compare with PBECs co-cultured with astrocytes and pericytes isolated from newborn rats with respect to TEER value and low passive permeability. The astrocytes and pericytes were grown both as contact and non-contact co-cultures as well as in triple culture to examine their effects on the PBECs for barrier formation as revealed by TEER, passive permeability, and expression patterns of tight junction proteins, efflux transporters and the transferrin receptor. This syngenic porcine in vitro BBB model is comparable to triple cultures using PBECs, rat astrocytes and rat pericytes with respect to TEER formation, low passive permeability, and expression of hallmark proteins signifying the brain endothelium (tight junction proteins claudin 5 and occludin, the efflux transporters P-glycoprotein (PgP) and breast cancer related protein (BCRP), and the transferrin receptor).

A Triple Culture Model of the Blood-Brain Barrier Using Porcine Brain Endothelial cells, Astrocytes and Pericytes.

Directory of Open Access Journals (Sweden)

Louiza Bohn Thomsen

Full Text Available In vitro blood-brain barrier (BBB models based on primary brain endothelial cells (BECs cultured as monoculture or in co-culture with primary astrocytes and pericytes are useful for studying many properties of the BBB. The BECs retain their expression of tight junction proteins and efflux transporters leading to high trans-endothelial electric resistance (TEER and low passive paracellular permeability. The BECs, astrocytes and pericytes are often isolated from small rodents. Larger species as cows and pigs however, reveal a higher yield, are readily available and have a closer resemblance to humans, which make them favorable high-throughput sources for cellular isolation. The aim of the present study has been to determine if the preferable combination of purely porcine cells isolated from the 6 months old domestic pigs, i.e. porcine brain endothelial cells (PBECs in co-culture with porcine astrocytes and pericytes, would compare with PBECs co-cultured with astrocytes and pericytes isolated from newborn rats with respect to TEER value and low passive permeability. The astrocytes and pericytes were grown both as contact and non-contact co-cultures as well as in triple culture to examine their effects on the PBECs for barrier formation as revealed by TEER, passive permeability, and expression patterns of tight junction proteins, efflux transporters and the transferrin receptor. This syngenic porcine in vitro BBB model is comparable to triple cultures using PBECs, rat astrocytes and rat pericytes with respect to TEER formation, low passive permeability, and expression of hallmark proteins signifying the brain endothelium (tight junction proteins claudin 5 and occludin, the efflux transporters P-glycoprotein (PgP and breast cancer related protein (BCRP, and the transferrin receptor.

Growth hormone-specific induction of the nuclear localization of porcine growth hormone receptor in porcine hepatocytes.

Science.gov (United States)

Lan, H N; Hong, P; Li, R N; Shan, A S; Zheng, X

2017-10-01

The phenomenon of nuclear translocation of growth hormone receptor (GHR) in human, rat, and fish has been reported. To date, this phenomenon has not been described in a domestic animal (such as pig). In addition, the molecular mechanisms of GHR nuclear translocation have not been thoroughly elucidated. To this end, porcine hepatocytes were isolated and used as a cell model. We observed that porcine growth hormone (pGH) can induce porcine GHR's nuclear localization in porcine hepatocytes. Subsequently, the dynamics of pGH-induced pGHR's nuclear localization were analyzed and demonstrated that pGHR's nuclear localization occurs in a time-dependent manner. Next, we explored the mechanism of pGHR nuclear localization using different pGHR ligands, and we demonstrated that pGHR's nuclear translocation is GH(s)-dependent. We also observed that pGHR translocates into cell nuclei in a pGH dimerization-dependent fashion, whereas further experiments indicated that IMPα/β is involved in the nuclear translocation of the pGH-pGHR dimer. The pGH-pGHR dimer may form a pGH-GHR-JAK2 multiple complex in cell nuclei, which would suggest that similar to its function in the cell membrane, the nuclear-localized pGH-pGHR dimer might still have the ability to signal. Copyright © 2017 Elsevier Inc. All rights reserved.

En verden af medier

DEFF Research Database (Denmark)

Tække, Jesper

2009-01-01

Bogen: 'En verden af medier – Medialiseringen af politik, sprog, religion og leg' anmeldes. Anmeldelsen kan læses på: http://ojs.statsbiblioteket.dk/index.php/mediekultur/article/view/1370/1499 Udgivelsesdato: 2009......Bogen: 'En verden af medier – Medialiseringen af politik, sprog, religion og leg' anmeldes. Anmeldelsen kan læses på: http://ojs.statsbiblioteket.dk/index.php/mediekultur/article/view/1370/1499 Udgivelsesdato: 2009...

Udforskning af journalistiske moralnormer

DEFF Research Database (Denmark)

Mogensen, Kirsten

2010-01-01

Kirsten Mogensen beskriver professionelle journalistiske normer. Med udgangspunkt i amerikansk tv's dækning af begivenhederne 11. september kombinerer Kirsten Mogensen kvalitative interviews og tekstanalyse for på den måde at beskrive professionelle journalistiske normer. Metoden er en kombinatio...... af halvstrukturerede, udforskende interviews med både kvantitative og kvalitative analyser af den faktiske dækning, og er primært udledt af Alf Ross' essay om normer og direktiver....

Radiation sensitivity of bacteria and virus in porcine xenoskin for dressing agent

International Nuclear Information System (INIS)

Jo, Eu-Ri; Jung, Pil-Mun; Choi, Jong-il; Lee, Ju-Woon

2012-01-01

In this study, gamma irradiation sensitivities of bacteria and viruses in porcine skin were evaluated to establish the optimum sterilization condition for the dressing material and a xenoskin graft. Escherichia coli and Bacillus subtilis were used as model pathogens and inoculated at 10 6 –10 7 log CFU/g. As model viruses, porcine parvovirus (PPV), bovine viral diarrhea virus (BVDV), and poliovirus were used and inoculated at 10 5 –10 6 TCID 50 /g into porcine skin. The D 10 value of E. coli was found to be 0.25±0.1 kGy. B. subtilis endospores produced under stressful environmental conditions showed lower radiation sensitivity as D 10 was 3.88±0.3 kGy in porcine skin. The D 10 values of PPV, BVDV, and poliovirus were found to be 1.73±0.2, 3.81±0.2, and 6.88±0.3 kGy, respectively. These results can offer the basic information required for inactivating pathogens by gamma irradiation and achieving dressing material and porcine skin grafts.

Pilotprojekt - In situ test af stimuleret aerob nedbrydning til oprensning af pesticidpunktkilder

DEFF Research Database (Denmark)

Tsitonaki, Katerina; Roost, Sandra; Larsen, Lars

Denne rapport omhandler et pilotforsøg i felten, hvor det er undersøgt, hvorvidt aerob nedbrydning af phenoxysyre-pesticider kan stimuleres ved tilsætning af ilt suppleret med tilsætning af specifikke pesticidnedbrydende bakterier. Forsøget er udført på en forurenet lokalitet, Skelstofte, hvor der...

Intelligent styring af dynamisk LED belysning

DEFF Research Database (Denmark)

Thorseth, Anders; Corell, Dennis Dan; Hansen, Søren Stentoft

Denne slutrapport giver en kort beskrivelse af arbejdet, der er udført af DTU Fotonik i projektet ”Intelligent styring af dynamisk LED belysning” støttet af EUDP. Arbejdet er udført i perioden 2011‐2012 i samarbejde med Lighten.......Denne slutrapport giver en kort beskrivelse af arbejdet, der er udført af DTU Fotonik i projektet ”Intelligent styring af dynamisk LED belysning” støttet af EUDP. Arbejdet er udført i perioden 2011‐2012 i samarbejde med Lighten....

Evaluering af afdeling H

DEFF Research Database (Denmark)

Kjær Minke, Linda; Birkmose, Sofie Meldal

2016-01-01

Som led i Kriminalforsorgens flerårsaftale 2013-2016 blev det åbne statsfængsel Søbysøgård i januar 2015 udvidet med 42 lukkede pladser i den nybyggede afdeling H. Nærværende evaluering kortlægger de erfaringer personalet og indsatte har gjort sig i løbet af det første år. Etableringen af den...... lukkede fængselsafdeling H giver en unik mulighed for at følge henholdsvis hvilke faktorer, der bidrager til udvikling af fængselskultur, og hvordan de respektive faktorer influerer på udvikling af fængselskulturen. Evalueringens metoder består af kvalitative og kvantitative metoder såsom dokumentanalyse...

Overførsel af viden ved flytning af produktion

DEFF Research Database (Denmark)

Madsen, Erik Skov; Riis, Jens Ove; Wæhrens, Brian Vejrum

2010-01-01

Empiriske studier i tre industrielle virksomheder har afsløret, at selv virksomheder med mange års erfaring i at overføre produktion til andre lande hovedsagelig fokuserer på planlægning af den fysiske overflytning af produktionsudstyr og fokuserer på den eksplicitte viden, der er tilknyttet prod...

Test af Software

DEFF Research Database (Denmark)

Dette dokument udgør slutrapporten for netværkssamarbejdet ”Testnet”, som er udført i perioden 1.4.2006 til 31.12.2008. Netværket beskæftiger sig navnlig med emner inden for test af indlejret og teknisk software, men et antal eksempler på problemstillinger og løsninger forbundet med test af...... administrativ software indgår også. Rapporten er opdelt i følgende 3 dele: Overblik. Her giver vi et resumé af netværkets formål, aktiviteter og resultater. State of the art af software test ridses op. Vi omtaler, at CISS og netværket tager nye tiltag. Netværket. Formål, deltagere og behandlede emner på ti...

Porcine endogenous retroviral nucleic acid in peripheral tissues is associated with migration of porcine cells post islet transplant.

Science.gov (United States)

Binette, Tanya M; Seeberger, Karen L; Lyon, James G; Rajotte, Ray V; Korbutt, Gregory S

2004-07-01

Porcine islets represent an alternative source of insulin-producing tissue, however, porcine endogenous retrovirus (PERV) remains a concern. In this study, SCID mice were transplanted with nonencapsulated (non-EC), microencapsulated (EC) or macroencapsulated (in a TheraCyte trade mark device) neonatal porcine islets (NPIs), and peripheral tissues were screened for presence of viral DNA and mRNA. To understand the role of an intact immune system in PERV incidence, mice with established NPI grafts were reconstituted with splenocytes. Peripheral tissues were screened for PERV and porcine DNA using PCR. Tissues with positive DNA were analyzed for PERV mRNA using RT-PCR. No significant difference was observed between non-EC and EC transplants regarding presence of PERV or porcine-specific DNA or mRNA. In reconstituted animals, little PERV or porcine DNA, and no PERV mRNA was detected. No PERV or porcine-specific DNA was observed in animals implanted with a TheraCyte trade mark device. In conclusion, an intact immune system significantly lowered the presence of PERV. Microencapsulation of islets did not alter PERV presence, however, macroencapsulation in the TheraCyte device did. Lower PERV incidence coincided with lower porcine DNA in peripheral tissues, linking the presence of PERV to migration of porcine cells.

Implementering af MultifunC

DEFF Research Database (Denmark)

Henze-Pedersen, Sofie; Kohl, Katrine Syppli; Oldrup, Helene

(MultifunC), som allerede havde vist lovende resultater i Norge og Sverige. I 2011 og 2012 åbnede to danske MultifunC-institutioner derfor i henholdsvis København og Aarhus. Denne rapport undersøger, hvordan implementeringen af MultifunC-programmet er forløbet i Danmark. Undersøgelsen viser, at ingen af de...... to danske institutioner lykkedes med at implementere MultifunC tilfredsstillende i løbet af implementeringsperioden. Det har fx været vanskeligt at implementere det tætte tværfaglige samarbejde mellem medarbejdere med forskellige fagprofessionelle baggrunde, som behandlingsprogrammet indebærer. Det har...... desuden været særdeles vanskeligt for de danske MultifunC-institutioner at få etableret det eksterne samarbejde med de sociale myndigheder i kommunerne, politiet, skoler og praktiksteder samt fritidstilbud i nærområdet, som også er en væsentlig del af behandlingsprogrammet. Undersøgelsen af finansieret af...

Test af spørgeskema

DEFF Research Database (Denmark)

Hermansen, Jonathan

2017-01-01

Denne bog gennemgår alle surveyarbejdets faser og giver en praktisk indføring i •design af undersøgelsen og udvælgelse af stikprøver, •formulering af spørgeskemaer samt indsamling og kodning af data, •metoder til at analysere resultaterne....

ANTICOAGULANT TREATMENT OF AF: FOCUS ON THE WARFARIN DOSAGE

Directory of Open Access Journals (Sweden)

Y. Kovbasniuk

2015-10-01

  Summary Authors analyzed the results of some studies of AF and warfarin dosing. Than investigation of 75 patient with AF (permanent type was performed. According to demographic, anthropometric data and related conditions (diabetes mellitus type 2, myocardial infarction, acute cerebrovascular ischemia, pulmonary embolism, alcohol abuse - drinking more than one ounce of ≥3 weekly predictive model of warfarin dosing was developed: warfarin dose = 0.097 * BMI - 0.03 * AGE + 0.30 *ASS.Status+ 0.02 *WC+ 1, 88. After testing of final model authors concluded what derived formula was representative, to determine the optimal dose of warfarin given to patient based on demographic characteristics. Considering all of the abovementioned, this model can be recommended for use determining the treatment strategy in patients with AF. Keywords: anticoagulant treatment, warfarin, atrial fibrillation.

Test af to versioner af Modflow-LGR til simulering af grundvandsstrømninger i områder med begravede dale – syntetisk studie

DEFF Research Database (Denmark)

Vilhelmsen, Troels Norvin; Christensen, Steen

.eks. findes i begravede dale. Manglende opløsning af geologiske aflejringer på lokal skala kan potentielt resultere i fejlagtige prædiktioner af konsekvensen af grundvandsindvinding på kildeplads niveau. For i højere grad at kunne forvalte disse grundvandsressourcerne skal nye metoder undersøges. Dette er...... specielt gældende for komplekse begravede dalsystemer, som interagerer med omkringliggende regionale grundvandsystemer. I dette syntetiske studie er to versioner af MODFLOW - Local Grid Refinment (LGR) testet til simulering af grundvandsstrømninger i områder med begravede dale. Forsøgene er gennemført som...

Per-Oral Endoscopic Myotomy (POEM) After Previous Laparoscopic Heller Myotomy Is Feasible and Safe in a Porcine Model.

Science.gov (United States)

Miles, Luke F; Frelich, Matthew J; Gould, Jon C; Dua, Kulwinder S; Jensen, Eric S; Kastenmeier, Andrew S

2015-10-01

We sought to evaluate the feasibility, safety, and difficulty of performing the per-oral endoscopic myotomy (POEM) procedure in the setting of a prior Heller myotomy using a survival porcine model. Four pigs underwent laparoscopic Heller myotomy with Dor partial anterior fundoplication followed by the POEM performed 4 weeks later. Two additional pigs served as controls, undergoing only the POEM. All procedures were completed without complications. The revisional POEM was not significantly more difficult than POEM controls based on procedure time, POEM procedure components, or procedure difficulty scores. Revisional POEM had a longer mean operative time when compared with Heller myotomy (126.0 vs. 83.8 min; PHeller myotomy is safe and feasible in the porcine model and has potential as an option for patients suffering from recurrent or persistent symptoms after failed surgical myotomy.

Insights into coronary collateral formation from a novel porcine semiacute infarction model.

Science.gov (United States)

Krackhardt, Florian; Harnoss, Jonathan M; Waliszewski, Matthias W; Ritter, Zully; Granzow, Susanne; Felsenberg, Dieter; Neumann, Konrad; Lerman, Lilian O; Hillmeister, Philipp; Gebker, Rolf; Paetsch, Ingo; Riediger, Fabian; Bramlage, Peter; Buschmann, Ivo R

2018-03-01

For patients with severe ischemic heart disease, complete revascularization by a percutaneous coronary intervention or coronary artery bypass grafting is often not achieved and may still cause residual angina. In case of progressive coronary artery occlusions, therapeutic arteriogenesis constitutes a promising strategy for increasing blood supply to the ischemic myocardium. Whether the formation of collaterals in the hypofused myocardium is angiogenetic in nature or based on preformed coronary artery anastomoses remains debatable. The objectives of this research were (i) the development of an appropriate research methodology to study a humanoid animal semiacute infarction model with low mortality and (ii) to answer the question of whether collateral revascularization follows a pre-existing 'blueprint'. A porcine model was chosen in which a step-wise vessel occlusion was performed by implantation of a copper stent into the distal left anterior descending artery. Vessel occlusion and collateral development were confirmed in vivo every 14 days up to day 56 by repeated coronary angiography and myocardial perfusion measurement using cardiac MRI. After the completion of the in-vivo imaging studies, animals were euthanized and collateral growth was evaluated using microcomputer tomography. Our porcine model of semiacute noninvasive coronary artery occlusion confirmed the existence of preformed coronary anastomoses and the proliferation of functional vessels in hypoperfused myocardium. Repetitive intra-animal MRIs showed the functional impact of these growing collaterals. The confirmation of preformed coronary anastomoses during the process of collateralization (natural bypasses) offers a preclinical avenue to carry out arteriogenetic pharmaceutical research in patients with ischemic heart disease.

Professor: Lær af Canada

DEFF Research Database (Denmark)

Greve, Carsten

2013-01-01

Transportdebatten: En mulig finansiering af fremtidige infrastrukturprojekter kunne være OPP. Danmark bør tage ved lære af erfaringer fra Canada.......Transportdebatten: En mulig finansiering af fremtidige infrastrukturprojekter kunne være OPP. Danmark bør tage ved lære af erfaringer fra Canada....

Didaktisk design af IKT og medier til fysioterapeutuddannelsen

DEFF Research Database (Denmark)

Nielsen, Kim

2012-01-01

eksisterer i den reale verden som love og regler for materielle/fysiske forhold ved fænomener, objekter, kultur, natur og vores krop, eller brug af objekter og fænomener som kan repræsentere teorier, modeller, love og regler fysisk frem for artikulation af den reale verden. Et valg som antager at viden er...... regler. Især æstetisk design, men også kirurgi, spilplatforme og nogle få IKT- og medieeksempler til uddannelsesområdet, bidrager med forskellige forslag til udvikling af didaktiske design som kunne matche undervisningens centrale menneskesyn, viden- og læringsopfattelse. Samtidigt peger ny neurologisk...

Formation of reactive aldehydes (MDA, HHE, HNE) during the digestion of cod liver oil: comparison of human and porcine in vitro digestion models.

Science.gov (United States)

Tullberg, Cecilia; Larsson, Karin; Carlsson, Nils-Gunnar; Comi, Irene; Scheers, Nathalie; Vegarud, Gerd; Undeland, Ingrid

2016-03-01

In this work, we investigated lipid oxidation of cod liver oil during gastrointestinal (GI) digestion using two types of in vitro digestion models. In the first type of model, we used human GI juices, while we used digestive enzymes and bile from porcine origin in the second type of model. Human and porcine models were matched with respect to factors important for lipolysis, using a standardized digestion protocol. The digests were analysed for reactive oxidation products: malondialdehyde (MDA), 4-hydroxy-trans-2-nonenal (HNE), and 4-hydroxy-trans-2-hexenal (HHE) by liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry (LC/APCI-MS), and for free fatty acids (FFA) obtained during the digestion by gas chromatography-mass spectrometry (GC-MS). The formation of the oxidation products MDA, HHE, and HNE was low during the gastric digestion, however, it increased during the duodenal digestion. The formation of the oxidation products reached higher levels when digestive juices of human origin were used (60 μM of MDA, 0.96 μM of HHE, and 1.6 μM of HNE) compared to when using enzymes and bile of porcine origin (9.8, and 0.36 μM of MDA; 0.16, and 0.026 μM of HHE; 0.23, and 0.005 μM of HNE, respectively, in porcine models I and II). In all models, FFA release was only detected during the intestinal step, and reached up to 31% of total fatty acids (FA). The findings in this work may be of importance when designing oxidation oriented lipid digestion studies.

Anmeldelse af World Drug Report 2005

DEFF Research Database (Denmark)

Møller, Kim

2005-01-01

Internationale udfordringer i spændingsfeltet mellem organiseret kriminalitet og narkotika. Værdien af det illegale narkotikamarked. Skabelsen af et Illicit Drug Index. Ny organisationsstruktur bag udgivelsen af denne WDR. Udgivelsesdato: December......Internationale udfordringer i spændingsfeltet mellem organiseret kriminalitet og narkotika. Værdien af det illegale narkotikamarked. Skabelsen af et Illicit Drug Index. Ny organisationsstruktur bag udgivelsen af denne WDR. Udgivelsesdato: December...

Thermal Ablation of the Pancreas With Intraoperative High-Intensity Focused Ultrasound: Safety and Efficacy in a Porcine Model.

Science.gov (United States)

Dupré, Aurélien; Melodelima, David; Pflieger, Hannah; Chen, Yao; Vincenot, Jérémy; Kocot, Anthony; Langonnet, Stéphan; Rivoire, Michel

2017-02-01

New focal destruction technologies such as high-intensity focused ultrasound (HIFU) may improve the prognosis of pancreatic ductal adenocarcinoma. Our objectives were to demonstrate the safety and efficacy of intraoperative pancreatic HIFU ablation in a porcine model. In a porcine model (N = 12), a single HIFU ablation was performed in either the body or tail of the pancreas, distant to superior mesenteric vessels. All animals were sacrificed on the eighth day. The primary objective was to obtain an HIFU ablation measuring at least 1 cm without premature death. In total, 12 HIFU ablations were carried out. These ablations were performed within 160 seconds and on average measured 20 (15-27) × 16 (8-26) mm. The primary objective was fulfilled in all but 1 pig. There were no premature deaths or severe complications. High-intensity focused ultrasound treatment was associated with a transitory increase in amylase and lipase levels, and pseudocysts were observed in half of the pigs without being clinically apparent. All ablations were well delimited at both gross and histological examinations. Intraoperative thermal destruction of porcine pancreas with HIFU is feasible. Reproducibility and safety have to be confirmed when applied close to mesenteric vessels and in long-term preclinical studies.

Ledelse af Organisationens Kreativitet

DEFF Research Database (Denmark)

Hedemann, Lars

2008-01-01

forskel, så er man som leder nødt til at arbejde mere målrettet med de medarbejdere som forventes at præstere et kreativt udbytte. Inspireret af analyseværktøjet "KEYS, Assessing the Work Environment for Creativity" udviklet af den amerikanske psykolog og ledelsesforsker ved Harvard University, Teresa...... Amabile, blev en undersøgelse derfor gennemført i udviklingsafdelingerne, Idéland og Idélab hos Bang og Olufsen, for at få et indblik i anvendeligheden af KEYS som inspirationskilde for succesfuld ledelse af organisationens kreativitet....

Digital formidling af kulturarv

DEFF Research Database (Denmark)

En af tidens store kulturelle dagsordner drejer sig om digital tilgængeliggørelse af kulturarv. Fortidens spor dukker op i nye former på nettet - næsten alle kulturinstitutioner arbejder med, og befolkningen har fået helt nye muligheder for at se med og deltage. Digital formidling af kulturarv...

Dicty_cDB: SSK247 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available .. 34 9.7 AB049544_1( AB049544 |pid:none) Porcine enterovirus 10 gene for RN... 3...4 9.7 AF363453_1( AF363453 |pid:none) Porcine enterovirus 9 strain UKG/4... 34 9.7 protein update 2009. 6.14

Epichlorhydrin og bisphenol A i mat-kontaktmaterialer af epoxylakeret metal og/eller af plast

DEFF Research Database (Denmark)

Petersen, Jens Højslev; Pedersen, Gitte Alsing; Foverskov, Annie

Det er ikke længere tilladt af bruge bisphenol A baseret polycarbonatplast til fremstilling af tåteflasker. Mattilsynet udtog i vinteren 2012/2013 7 typer af sutteflasker som blev kontrolleret for om de indeholdt polycarbonatbaserede plastdele. I alt 28 enkeltdele blev kontrolleret ved infrarød...... spektrometri. Herudover blev 6 tutekopper, bestående af 38 enkeltdele, analyseret med samme teknik. Der blev ikke påvist bisphenol A baseret plast ved analyserne. Dåser, bægre, tuber og skruelåg kan være lakeret på matkontaktsiden med epoxylakker fremstillet af monomererne epichlorhydrin og bisphenol A. Alle...... 50 enkeltdele fra disse prøver blev undersøgt ved infrarød spektrometri og 9 af prøverne viste tegn på at indeholde epoxylak. Disse prøver blev undersøgt ved en ”worst case” ekstraktionstest hvor der kunne påvises små indhold af bisphenol A i ekstrakterne mens der ikke kunne påvises epichlorhydrin...

ARCgame - gamification af undervisning

DEFF Research Database (Denmark)

Jensen, Camilla Gyldendahl

2014-01-01

Gamification er et begreb, der anvendes til at beskrive brugen af spilelementer i andre miljøer for at forbedre brugernes oplevelse. Følgende tekst har til formål at behandle emnet i forhold til af opnå en forståelse for de elementer der indvirker på en ”gamification af et undervisningsforløb”, her...

Hvad griner du af?

DEFF Research Database (Denmark)

Lorenzen, Tobias Hiort

2012-01-01

latterliggørelse indenfor en sådan teori. Sociologiens begreb om ”un-laughter” angives som en mulig udvej af dette problem. I forlængelse af dette kritiseres den moderne humor-psykologis lære om populationers humor-patologi og til slut argumenteres der for at en moderne dannelse af humor må have en moralsk...

Development of left ventricular hypertrophy in a novel porcine model of mitral regurgitation

DEFF Research Database (Denmark)

Ravn, Nathja; Zois, Nora Elisabeth; Moesgaard, Sophia Gry

2014-01-01

OBJECTIVES: We aimed to develop a porcine model for chronic nonischemic mitral regurgitation (MR) to investigate left ventricular (LV) enlargement and eccentric hypertrophy. DESIGN: Nonischemic MR was induced in 30 pigs by open-chest immobilization of the posterior mitral leaflet by transannular...... (LVIDd) from baseline to follow-up was significantly higher in the sMR group compared to that of the control group (P = 0.0017). Furthermore, LV weight was significantly increased in the mMR (P = 0.047) and the sMR (P = 0.0087) groups compared to that of the control group. CONCLUSIONS: A new model...

Skoling af lyst

DEFF Research Database (Denmark)

Bjerg, Helle

2011-01-01

På baggrund af interview med tre elevgenerationer fremanalyseres skolens virkningsfuldhed i skoling af lyst i et historisk perspektiv. I afhandlingen udvikles to teoretisk-analytiske optikker: Skolen som ideologisk rum - med inspiration fra Slavoj Zizeks ideologikritik, samt Skolen som affektivt...

Chronic Porcine Two-Hit Model with Hemorrhagic Shock and textitPseudomonas aeruginosa Sepsis

OpenAIRE

Eissner, B.;Matz, K.;Smorodchenko, A.;Röschmann, A.;Specht, B. U. v.

2016-01-01

Background: Sepsis is still a major cause of death despite well-developed therapeutical strategies such as antibiotics and supportive medication. The aim of this study was to characterize the long-term effects of a two-hit porcine sepsis model with a hemorrhagic shock as ‘first hit’ followed by a Pseudomonas aeruginosa infusion as ‘second hit’. Materials and Methods: Twelve juvenile healthy pigs were anesthetized and hemodynamically monitored. The two-hit group (n = 6) underwent a hemorrhagic...

Korrekt implementering af Business Intelligence skaber merværdi

DEFF Research Database (Denmark)

Gaardboe, Rikke; Fladkjær, Henrik Find; Nielsen, Christian

2016-01-01

I disse år fokuserer virksomheder på at implementere Business Intelligence (BI), så de stigende mængder af data og opsamlet forretningsinformation kan anvendes som ledelsesinformation. I denne artikel fokuseres på, hvordan virksomheder opnår merværdi ved implementering af BI, idet mange virksomhe......I disse år fokuserer virksomheder på at implementere Business Intelligence (BI), så de stigende mængder af data og opsamlet forretningsinformation kan anvendes som ledelsesinformation. I denne artikel fokuseres på, hvordan virksomheder opnår merværdi ved implementering af BI, idet mange...... virksomheder tilsyneladende har udfordringer med at opnå dette. Formålet er at analysere, hvordan virksomheder kan implementere Business Intelligence, således at de opnår merværdi. I artiklen udvikles en model for balanceret merværdi, hvor merværdien anskues på både organisations-, leder/beslutningstager- samt...... analyseres dette ud fra virksomhedernes modenhedsstadie, transformation fra data til analyse samt merværdi. Artiklens forskningsbidrag er en model til identifikation af dimensionaliseret merværdi, og artiklens bidrag til praksis er at analysere ud fra litteraturstudier, hvordan virksomheder kan opnå højere...

Dicty_cDB: CHN788 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available ) Clostridium botulinum F str. La... 35 7.2 AB049544_1( AB049544 |pid:none) Porcine enterovirus 10 gene for ...RN... 34 9.4 AF363453_1( AF363453 |pid:none) Porcine enterovirus 9 strain UKG/4... 34 9.4 protein update 200

Dicty_cDB: VHM533 [Dicty_cDB

Lifescience Database Archive (English)

Full Text Available num A3 str. L... 35 7.5 AB049544_1( AB049544 |pid:none) Porcine enterovirus 10 ge...ne for RN... 34 9.8 AF363453_1( AF363453 |pid:none) Porcine enterovirus 9 strain UKG/4... 34 9.8 protein upd

Udviklingen af rumopfattelsen 1600-2000

DEFF Research Database (Denmark)

Brock, Steen

2010-01-01

Hovedtræk af rumopfattelsens idehistorie fra Descartes til Wittgenstein med speciel vægt på Kant, værkbegrebet, modelbegrebet og den fænomenologiske opfattelse af rummet som handlingens sted......Hovedtræk af rumopfattelsens idehistorie fra Descartes til Wittgenstein med speciel vægt på Kant, værkbegrebet, modelbegrebet og den fænomenologiske opfattelse af rummet som handlingens sted...

Undersøgelsen af brugernes evaluering af kontorpakker på Århus Sygehus og i Psykiatrien i Århus

DEFF Research Database (Denmark)

Hørlück, Jens

En analyse af brugernes opfattelse af kvaliteten af tre office pakker: MS Office 2003 / 2007 og OpenOffice.  ......En analyse af brugernes opfattelse af kvaliteten af tre office pakker: MS Office 2003 / 2007 og OpenOffice.  ...

7 CFR 1230.611 - Porcine animal.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Porcine animal. 1230.611 Section 1230.611 Agriculture... CONSUMER INFORMATION Procedures for the Conduct of Referendum Definitions § 1230.611 Porcine animal. The term Porcine animal means a swine, that is raised: (a) As a feeder pig, that is, a young pig sold to...

Supervision af psykoterapi

DEFF Research Database (Denmark)

SUPERVISION AF PSYKOTERAPI indtager en central position i uddannelsen og udviklingen af psykoterapeuter. Trods flere lighedspunkter med psykoterapi, undervisning og konsultation er psykoterapisupervision et selvstændigt virksomhedsområde. Supervisor må foruden at være en trænet psykoterapeut kende...... supervisionens rammer og indplacering i forhold til organisation og samfund. En række kapitler drejer sig om supervisors opgaver, roller og kontrolfunktion, supervision set fra supervisandens perspektiv samt betragtninger over relationer og processer i supervision. Der drøftes fordele og ulemper ved de...... forskellige måder, hvorpå en sag kan fremlægges. Bogens første del afsluttes med refleksioner over de etiske aspekter ved psykoterapisupervision. Bogens anden del handler om de særlige forhold, der gør sig gældende ved supervision af en række specialiserede behandlingsformer eller af psykoterapi med bestemte...

Anmeldelse af Evolution, Literature and Film

DEFF Research Database (Denmark)

Grodal, Torben Kragh

2011-01-01

Diskussion af basisproblemer i evolutionær fiktionsteori med udgangspunkt i en anmeldelse af Evolution, Literature and Film......Diskussion af basisproblemer i evolutionær fiktionsteori med udgangspunkt i en anmeldelse af Evolution, Literature and Film...

Porcine cluster of differentiation (CD) markers 2018 update.

Science.gov (United States)

Dawson, Harry D; Lunney, Joan K

2018-06-01

Pigs are a major source of food worldwide; preventing and treating their infectious diseases is essential, requiring a thorough understanding of porcine immunity. The use of pigs as models for human physiology is a growing area; progress in this area has been limited because the immune toolkit is not robust. The international community has established cluster of differentiation (CD) markers for assessing cells involved in immunity as well as characterizing numerous other cells like stem cells. Overall, for humans 419 proteins have been designated as CD markers, each reacting with a defined set of antibodies (Abs). This paper summarizes current knowledge of swine CD markers and identifies 359 corresponding CD proteins in pigs. A broad-based literature and vendor search was conducted to identify defined sets of monoclonal (mAbs) and polyclonal Abs (pAbs) reacting with porcine CD markers along with other reagents (fusion proteins, ELISAs, PCR assays, and gene edited cell and pig models). This process identified over 800 reagents that are reportedly reactive with 266 pig CD markers. Despite this number, there is a great need to develop and characterize additional CD marker reagents, particularly mAbs, for pig research. There are numerous high priority targets: reagents for the characterization of porcine innate lymphoid cells, polarized macrophages and T regulatory cells and for the detection of porcine CD45 isoforms. Overall, improved technologies and genomics have contributed to dramatic increases in our knowledge of the pig, its immune system, disease and vaccine responses, and utility as a biomedical model. The development of more CD reagents will clearly advance these initiatives. Published by Elsevier Ltd.

Porcine head response to blast.

Science.gov (United States)

Shridharani, Jay K; Wood, Garrett W; Panzer, Matthew B; Capehart, Bruce P; Nyein, Michelle K; Radovitzky, Raul A; Bass, Cameron R 'dale'

2012-01-01

Recent studies have shown an increase in the frequency of traumatic brain injuries related to blast exposure. However, the mechanisms that cause blast neurotrauma are unknown. Blast neurotrauma research using computational models has been one method to elucidate that response of the brain in blast, and to identify possible mechanical correlates of injury. However, model validation against experimental data is required to ensure that the model output is representative of in vivo biomechanical response. This study exposes porcine subjects to primary blast overpressures generated using a compressed-gas shock tube. Shock tube blasts were directed to the unprotected head of each animal while the lungs and thorax were protected using ballistic protective vests similar to those employed in theater. The test conditions ranged from 110 to 740 kPa peak incident overpressure with scaled durations from 1.3 to 6.9 ms and correspond approximately with a 50% injury risk for brain bleeding and apnea in a ferret model scaled to porcine exposure. Instrumentation was placed on the porcine head to measure bulk acceleration, pressure at the surface of the head, and pressure inside the cranial cavity. Immediately after the blast, 5 of the 20 animals tested were apneic. Three subjects recovered without intervention within 30 s and the remaining two recovered within 8 min following respiratory assistance and administration of the respiratory stimulant doxapram. Gross examination of the brain revealed no indication of bleeding. Intracranial pressures ranged from 80 to 390 kPa as a result of the blast and were notably lower than the shock tube reflected pressures of 300-2830 kPa, indicating pressure attenuation by the skull up to a factor of 8.4. Peak head accelerations were measured from 385 to 3845 G's and were well correlated with peak incident overpressure (R(2) = 0.90). One SD corridors for the surface pressure, intracranial pressure (ICP), and head acceleration are

Allogeneic amniotic membrane-derived mesenchymal stromal cell transplantation in a porcine model of chronic myocardial ischemia

Directory of Open Access Journals (Sweden)

Kimura M

2012-01-01

Full Text Available Introduction. Amniotic membrane contains a multipotential stem cell population and is expected to possess the machinery to regulate immunological reactions. We investigated the safety and efficacy of allogeneic amniotic membrane-derived mesenchymal stromal cell (AMSC transplantation in a porcine model of chronic myocardial ischemia as a preclinical trial. Methods. Porcine AMSCs were isolated from amniotic membranes obtained by cesarean section just before delivery and were cultured to increase their numbers before transplantation. Chronic myocardial ischemia was induced by implantation of an ameroid constrictor around the left circumflex coronary artery. Four weeks after ischemia induction, nine swine were assigned to undergo either allogeneic AMSC transplantation or normal saline injection. Functional analysis was performed by echocardiography, and histological examinations were carried out by immunohistochemistry 4 weeks after AMSC transplantation. Results. Echocardiography demonstrated that left ventricular ejection fraction was significantly improved and left ventricular dilatation was well attenuated 4 weeks after AMSC transplantation. Histological assessment showed a significant reduction in percentage of fibrosis in the AMSC transplantation group. Injected allogeneic green fluorescent protein (GFP-expressing AMSCs were identified in the immunocompetent host heart without the use of any immunosuppressants 4 weeks after transplantation. Immunohistochemistry revealed that GFP colocalized with cardiac troponin T and cardiac troponin I. Conclusions. We have demonstrated that allogeneic AMSC transplantation produced histological and functional improvement in the impaired myocardium in a porcine model of chronic myocardial ischemia. The transplanted allogeneic AMSCs survived without the use of any immunosuppressants and gained cardiac phenotype through either their transdifferentiation or cell fusion.

Strategisk ledelse af evalueringskulturen

Directory of Open Access Journals (Sweden)

Gitte Orlowitz

2016-02-01

Full Text Available Hvis evaluering skal anvendes til at udvikle uddannelser, er en af ledelsen vigtigste opgaver at udvikle en strategi for udvikling af evalueringskulturen – herunder strategier for udvikling af kommunikation og evalueringskapacitet. Den strategiske ledelse kan i den sammenhæng anvendes til at håndtere de ofte modsatte krav, som eksterne og interne interessenter stiller til evaluering.

Irreversible electroporation of the pancreas is feasible and safe in a porcine survival model.

Science.gov (United States)

Fritz, Stefan; Sommer, Christof M; Vollherbst, Dominik; Wachter, Miguel F; Longerich, Thomas; Sachsenmeier, Milena; Knapp, Jürgen; Radeleff, Boris A; Werner, Jens

2015-07-01

Use of thermal tumor ablation in the pancreatic parenchyma is limited because of the risk of pancreatitis, pancreatic fistula, or hemorrhage. This study aimed to evaluate the feasibility and safety of irreversible electroporation (IRE) in a porcine model. Ten pigs were divided into 2 study groups. In the first group, animals received IRE of the pancreatic tail and were killed after 60 minutes. In the second group, animals received IRE at the head of the pancreas and were followed up for 7 days. Clinical parameters, computed tomography imaging, laboratory results, and histology were obtained. All animals survived IRE ablation, and no cardiac adverse effects were noted. Sixty minutes after IRE, a hypodense lesion on computed tomography imaging indicated the ablation zone. None of the animals developed clinical signs of acute pancreatitis. Only small amounts of ascites fluid, with a transient increase in amylase and lipase levels, were observed, indicating that no pancreatic fistula occurred. This porcine model shows that IRE is feasible and safe in the pancreatic parenchyma. Computed tomography imaging reveals significant changes at 60 minutes after IRE and therefore might serve as an early indicator of therapeutic success. Clinical studies are needed to evaluate the efficacy of IRE in pancreatic cancer.

Evaluering af FIU`s akademiuddannelser

DEFF Research Database (Denmark)

Hansen, Agnete Meldgaard; Nielsen, Kurt Aagaard

I rapporten præsenteres en empirisk evaluerinf af to nye uddannelser i FIUs regi. I forlængelse af evalueringen præsenteres en forskningsmæssig analyse a uddannelsernes rolle i fagbevægelsens modernisering......I rapporten præsenteres en empirisk evaluerinf af to nye uddannelser i FIUs regi. I forlængelse af evalueringen præsenteres en forskningsmæssig analyse a uddannelsernes rolle i fagbevægelsens modernisering...

Udvikling af systemleverancer, parametri og BIM

DEFF Research Database (Denmark)

Kortegaard, Per

2016-01-01

Seminaret rummede indlæg fra både nogle af projektets tidligere ph.d.-studerende og forskellige kompetente professionelle personer fra forskellige grene af praksis. Seminaret gav samlet et fint billede af status indenfor udviklingen af systemleverancer og deres anvendelse i relation til BIM...

Administration af informationsmodeller

DEFF Research Database (Denmark)

Hundebøl, Jesper

2013-01-01

En informationsmodel er i denne sammenhæng en digital repræsentation af et givent byggeri. Både offentlige og private bygherrer anvender informationsmodeller, når der 'bygges digitalt'. I denne artikel undersøges det, hvilke konkrete udfordringer der følger af digitaliseringen, hvor gennemgribend...

Dansk oversættelse af MiDAS, et redskab til assessment af musikterapi for personer med demens

DEFF Research Database (Denmark)

Ridder, Hanne Mette Ochsner; Lykkegaard, Chris; McDermott, Orii

2018-01-01

Music in Dementia Assessment Scales, MiDAS, er udviklet til at vurdere ændringer i trivsel hos borgere med demens som har deltaget i musikterapi. Målingen udføres af 1) personale før og efter musikterapien for at vurdere om der er en indflydelse i hverdagssituationer efterfølgende, eller af 2......) musikterapeuten umiddelbart efter sessionen for at vurdere forandringer i selve sessionen. Der har været stor interesse for at anvende MiDAS i flere Europæiske lande, herunder Danmark. Derfor undersøgte udviklerne af MiDAS hvilke procedurer der anvendes til oversættelse og kulturel tilpasning af...... assessmentredskaber, og anbefalede på den baggrund en ti-trins procedure. Vi anvendte denne procedure til oversættelsen af MiDAS og således udviklingen af den danske MiDAS-DK. Den danske version af MiDAS er afprøvet af mere end 20 fagpersoner på baggrund af 296 udfyldte MiDAS-skemaer. Målet med artiklen er...

Fremtidens biblioteksbetjening af børn

DEFF Research Database (Denmark)

Høyrup, Helene

2008-01-01

Rapporten er resultatet af et tværministerielt udvalgsarbejde nedsat af Kulturministeriet.  Kommissoriet har været at nytænke biblioteksbetjeningen af børn, så den passer til vidensamfundet.  Udvalgsarbejdet munder ud i ti bud for fremtidens biblioteksbetjening af børn....

Beregning af luftlydisolation mellem to rum

DEFF Research Database (Denmark)

Donovan, A.

Meddelelsen bringer resultaterne af en forundersøgelse af to beregningsmetoder. Den ene metode belyses nærmere gennem nogle forenklede eksempler, der viser omfanget af regnearbejdet og peger på nogle af de vanskeligheder det vil indebære at opstille en praktisk anvendelig beregningsmetode....

Regulering af det psykiske arbejdsmiljø

DEFF Research Database (Denmark)

Nielsen, Klaus T.

nationale informationer om reguleringen af det psykiske arbejdsmiljø. Rapport er bygget op med ◊ et internationalt overblik over regulering af psykisk arbejdsmiljø ◊ en bredere diskussion af reguleringens dilemmaer ◊ en gennemgang af den aktuelle danske udvikling ◊ udfordringerne på området...

Isolation and culture of porcine neural progenitor cells from embryos and pluripotent stem cells

DEFF Research Database (Denmark)

Rasmussen, Mikkel Aabech; Hall, Vanessa Jane; Hyttel, Poul

2013-01-01

from porcine embryos or induced pluripotent stem cells is presented. The neural induction is performed in coculture and the isolation of rosette structures is carried out manually to ensure a homogenous population of NPCs. Using this method, multipotent NPCs can be obtained in approximately 1 month......The isolation and culture of neural progenitor cells (NPCs) from pluripotent stem cells has facilitated in vitro mechanistic studies of diseases related to the nervous system, as well as discovery of new medicine. In addition, NPCs are envisioned to play a crucial role in future cell replacement...... therapy. The pig has become recognized as an important large animal model and establishment of in vitro-derived porcine NPCs would allow for preclinical safety testing by transplantation in a porcine biomedical model. In this chapter, a detailed method for isolation and in vitro culture of porcine NPCs...

Anvendeligheden af GIS

DEFF Research Database (Denmark)

Winstrup, Mie; Levin, Gregor

2011-01-01

Fragmentering af naturen er en trussel mod biodiversiteten, og etablering af økologiske forbindelser/korridorer mellem naturområderne er én måde hvorved nedgangen i biodiversitet kan stoppes. Med Næstved Kommune som case-område har jeg undersøgt, hvordan analyser i GIS kan bruges til at udvælge...... omdannet til reelle korridorer, hvor bredden afhænger af arealdækket som forbindelserne krydser. I implementerings øjemed er det anvendeligt at vide om nogle forbindelser er særlig vigtige for at skabe mere sammenhængende natur. GIS er anvendeligt hertil, idet GIS kan bruges til at bestemme den enkelte...

Kants Kritik af dømmekraften

DEFF Research Database (Denmark)

Introduktion til Kants Kritik af dømmekraften bestående af otte artikler, der følger værkets egen tematiske opbygning......Introduktion til Kants Kritik af dømmekraften bestående af otte artikler, der følger værkets egen tematiske opbygning...

Pædagogisk brug af test

DEFF Research Database (Denmark)

Nordenbo, Sven Erik; Allerup, Peter; Andersen, Hanne Leth

Publikationen er resultatet af et systematisk review foretaget i regi af Dansk Clearinghouse for Uddannelsesforskning. Reviewets grundspørgsmål er: Hvordan kan grundskolelæreres individ- og klassecentrerede brug af data fra test forbedre læreres didaktiske og/eller fagdidaktiske tiltag i klasser...... med almindelige elever? Spørgsmålet vedrører alene testtyper, som indgår i nationale test i de nordiske lande. Og hvordan indvirker indførelsen af testning på læreres didaktiske beslutninger og elevers læringsadfærd? Vi fandt frem til at grundskolelæreres individ- og klassecentrerede brug af testdata...... i klasser med almindelige elever forbedres, når de didaktiske og/eller fagdidaktiske tiltag bygger på test, som lærerne oplever et medejerskab for. Vi fandt fremtil, at introduktion af test fra instanser over klasseniveau med det formål at indvirke på undervisning og elevers læringsadfærd har...

Anvendelse af koldpresset rapsolie på varebiler

DEFF Research Database (Denmark)

Nielsen, Claus Suldrup; Ivarsson, Anders; Schramm, Jesper

Denne rapport sammenfatter resultaterne af undersøgelserne omkring anvendelse af koldpresset rapsolie på varebiler. Undersøgelsen, som er udført af Danmarks Tekniske Universitet, Institut for Mekanisk Teknologi, er en del af et større projekt, som er finansieret af Trafikstyrelsens (tidligere Fær...... Færdselsstyrelsen) tilskudsordning til forsøg med biodiesel. Projektet har været administreret af Odense Kommune....

Studerende og underviseres brug af digitale medier - En kvantitativ undersøgelse af Aarhus Universitets studerende og underviseres brug af digitale medier og internettet anno 2008

Directory of Open Access Journals (Sweden)

Maria Hvid Stenalt

2009-06-01

• Hvilke medier anvender universitetsstuderende generelt? • Ligner undervisernes medievalg/forbrug de studerendes? • Hvilke medier inddrager underviserne i undervisningen? Nærværende artikel vil forsøge at besvare disse spørgsmål på baggrund af en kvantitativ spørgeskemaundersøgelse af Aarhus Universitets studerende og underviseres brug af digitale medier og internettet. Undersøgelsen blev gennemført i efteråret 2008 af E-læringsenheden ved Aarhus Universitet som led i en almen kvalitetssikring på media- og e-læringsområdet, samt som led i en behovsafklaring vedrørende etablering af et medie- og podcastarkiv. E-læringsenheden er en administrativ enhed, der teknisk og pædagogisk understøtter underviseres inddragelse af digitale medier i undervisningen.

Monoclonal antibodies specific to heat-treated porcine blood.

Science.gov (United States)

Raja Nhari, Raja Mohd Hafidz; Hamid, Muhajir; Rasli, Nurmunirah Mohamad; Omar, Abdul Rahman; El Sheikha, Aly Farag; Mustafa, Shuhaimi

2016-05-01

Porcine blood is potentially being utilized in food as a binder, gelling agent, emulsifier or colorant. However, for certain communities, the usage of animal blood in food is strictly prohibited owing to religious concerns and health reasons. This study reports the development of monoclonal antibodies (MAbs) against heat-treated soluble proteins (HSPs) of autoclaved porcine blood; characterization of MAbs against blood, non-blood and plasma from different animal species using qualitative indirect non-competitive enzyme-linked immunosorbent assay (ELISA); and immunoblotting of antigenic components in HSPs of porcine blood. Fifteen MAbs are specific to heat-treated and raw porcine blood and not cross-reacted with other animal blood and non-blood proteins (meat and non-meat). Twelve MAbs are specific to porcine plasma, while three MAbs specific to porcine plasma are cross-reacted with chicken plasma. Immunoblotting revealed antigenic protein bands (∼60, ∼85-100 and ∼250 kDa) in porcine blood and plasma recognized by the MAbs. Selection of MAbs that recognized 60 kDa HSPs of porcine blood and plasma as novel monoclonal antibodies would be useful for detection of porcine plasma in processed food using the immunoassay method. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

Prøvetagning af grundvand

DEFF Research Database (Denmark)

Sørensen, Inga

Bogen fokuserer på prøveatgning af grundvand og drikkevand til forskellige formål såsom undersøgelse af forurenede grunde, grundvandsmonitering samt produktion af drikkevand. Da grundvand i sagens natur findes under jordoverfladen, beskriver bogen også væsentlige forhold om hydrogeologi og...

Global opvarmning neutraliserer effekten af parasitisme på strandsnegles konsumption af makroalger

DEFF Research Database (Denmark)

Larsen, Martin H.; Mouritsen, Kim Nørgaard

Det er veldokumenteret, at parasitter kan påvirke værternes adfærd, overlevelse, reproduktion, fysiologi og evolution. Disse parasit-vært interaktioner kan føre til radikale ændringer af naturlige samfunds struktur og diversitet. Eksperimenter har eksempelvis vist, at trematoder indirekte kan ændre...... strukturen af makroalgesamfund ved at reducere konsumptionsraten hos den herbivore strandsnegl Littorina littorea. Det er endvidere vist, at produktionen af trematode-larver (cercarier) i sneglens fordøjelseskanal og gonade kompleks øges kraftigt når vandtemperaturen stiger. Man kan derfor forvente...

Porcine respiratory disease complex: Interaction of vaccination and porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, and Mycoplasma hyopneumoniae.

Science.gov (United States)

Chae, Chanhee

2016-06-01

Porcine respiratory disease is a multifactorial and complex disease caused by a combination of infectious pathogens, environmental stressors, differences in production systems, and various management practices; hence the name porcine respiratory disease complex (PRDC) is used. Porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and Mycoplasma hyopneumoniae are considered to be the most important pathogens that cause PRDC. Although interactions among the three major respiratory pathogens are well documented, it is also necessary to understand the interaction between vaccines and the three major respiratory pathogens. PRRSV and M. hyopneumoniae are well known to potentiate PCV2-associated lesions; however, PRRSV and mycoplasmal vaccines can both enhance PCV2 viraemia regardless of the effects of the actual PRRSV or M. hyopneumoniae infection. On the other hand, M. hyopneumoniae potentiates the severity of pneumonia induced by PRRSV, and vaccination against M. hyopneumoniae alone is also able to decrease PRRSV viraemia and PRRSV-induced lung lesions in dually infected pigs. This review focuses on (1) interactions between PCV2, PRRSV, and M. hyopneumoniae; and (2) interactions between vaccines and the three major respiratory pathogens. Copyright © 2015 Elsevier Ltd. All rights reserved.

Måling af stress hos mink

DEFF Research Database (Denmark)

Malmkvist, Jens

2010-01-01

Sammendrag Stress indgår som et element ved vurdering af dyrevelfærd. Endvidere kan stress mål anvendes i undersøgelser, hvor forskellige typer af avl, burmiljø og pasning af mink sammenlignes. Men hvad er stress egentlig og hvordan måles det? Er unormal adfærd koblet til stress hos mink? På...... temadagen præsenteres nye resultater og metoder til at vurdere stress hos mink. I en metode måles koncentrationen af nedbrydningsprodukter af hormonet cortisol i gødning fra mink. Der er en række fordele ved denne metode, frem for blodprøvetagning. Forsøg viste, at hovedparten af cortisol udskilles i fæces...... (83 %) frem for i urin (17 %), samt at omsætningen af radioaktivt mærket cortisol ikke er forskellig mellem mink med hhv. lav og høj forekomst af stereotypi. Denne viden kan bruges til bedre at forstå koblingen f.eks. unormal adfærd og stress hos mink....

På sporet af de gyldne opskrifter?

DEFF Research Database (Denmark)

Poulfelt, Flemming

2015-01-01

Børsen Ledelse. Markedet for kogebøger er stort. For der produceres hvert år et hav nye af slagsen. Nogle udgives af kendte kokke, nogle af særlige eksperter inden for kost og motion, og andre af personer, der har en ægte interesse inden for gastronomi og mad. På ledelsesområdet findes en...

A Novel Porcine Model of Septic Shock Induced by Acute Respiratory Distress Syndrome due to Methicillin-resistant Staphylococcus aureus

Directory of Open Access Journals (Sweden)

Shuo Wang

2017-01-01

Conclusions: In the present study, we developed a novel porcine model of septic shock induced by ARDS due to severe MRSA pneumonia with characteristic hyperdynamic and hypodynamic phases in 24 h, which mimicked the hemodynamic changing of septic shock in human.

[An experimental model of transgastric ooforectomy using a porcine model].

Science.gov (United States)

Tomulescu, V; Gheorghe, C; Piţigoi, D; Kosa, A; Ciocarlan, M; Pietrăreanu, D; Turcu, F; Copăescu, C; Droc, G; Popescu, H; Grigorescu, B; Stănciulea, O; Herlea, V; Popescu, I

2010-01-01

Transabdominal routes for surgery entail general anaesthesia with its inherent risks and complications (prolonged hospital stay, abdominal incisions that may be difficult in obese patients). Minimally invasive procedures require shorter hospitalization, have shorter recovery periods, less postoperative discomfort, and lower morbidity and complications. The purpose of this study was to use a porcine model to determine the feasibility and the safety of organ resection (oophorectomy and tubectomy). 10 Big White pigs between 25-30 kg underwent transgastric ooforectomy. The first 5 cases were performed in a hybrid procedure (laparoscopic-NOTES) in order to have a better control and supervise the maneuvers done by the mobile endoscope and to guide in the abdominal cavity. Adnexectomy was possible in all ten experiments. Full operative time (from starting endoscopy to complete gastrectomy closing) was 180 min to 270 min. The gastric defect closing was the most difficult manoever lasting from 10 min with OTSC clips to 100 using endoloops and clips. The animals have tolerated well the experiments and there have been no remarkable incidents during our 10 experments. In only one case a bleeding from gastotomy required electric coagulation. Transgastric ooforectomy in an experimental model is a procedure that requires advanced laparoscopical and endoscopical skills. Our early results are promissing. Its application in humans needs further confirmation of the method.

Påvisning af PCV2

DEFF Research Database (Denmark)

Neumann, Katrine; Buse, Katja Strøm; Hjulsager, Charlotte Kristiane

2018-01-01

Mængden af PCV2-virus i poolede serumprøver kan anvendes til at finde grise med meget PCV2-virus, hvorimod mængden af PCV2-virus i spyt og fæces er velegnede til at vurdere tilstedeværelsen af eller tidspunktet for en begyndende PCV2-infektion...

Påvisning af PCV2

DEFF Research Database (Denmark)

Neumann, Katrine; Buse, Katja Strøm; Hjulsager, Charlotte Kristiane

Mængden af PCV2-virus i poolede serumprøver kan anvendes til at finde grise med meget PCV2-virus, hvorimod mængden af PCV2-virus i spyt og fæces er velegnede til at vurdere tilstedeværelsen af eller tidspunktet for en begyndende PCV2-infektion...

Topological ferrimagnetic behaviours of coordination polymers containing manganese(II) chains with mixed azide and carboxylate bridges and alternating F/AF/AF'/AF'/AF interactions.

Science.gov (United States)

Wang, Yan-Qin; Liu, Hou-Ting; Qi, Yan; Gao, En-Qing

2014-08-21

Two Mn(ii) complexes with azide and a new zwitterionic tetracarboxylate ligand 1,2,4,5-tetrakis(4-carboxylatopyridinium-1-methylene)benzene (L(1)), {[Mn5(L(1))2(N3)8(OH)2]·12H2O}n () and {[Mn5(L(1))2(N3)8(H2O)2](ClO4)2·6H2O}n (), have been synthesized and characterized crystallographically and magnetically. and contain similar alternating chains constructed by azide and carboxylate bridges. The independent sets of bridges alternate in an ABCCB sequence between adjacent Mn(ii) ions: (EO-N3)2 double bridges (EO = end-on) (denoted as A), [(EO-N3)(OCO)2] triple bridges (denoted as B) and [(EO-N3)(OCO)] double bridges (denoted as C). The alternating chains are interlinked into 2D coordination networks by the tetrapyridinium spacers. Magnetic studies demonstrate that the magnetic coupling through the double EO azide bridges is ferromagnetic and that through mixed azide/carboxylate bridges is antiferromagnetic. The unprecedented F/AF/AF'/AF'/AF coupling sequence along the chain dictates an uncompensated ground spin state (S = 5/2 per Mn5 unit) and leads to one-dimensional topological ferrimagnetism, which features a minimum in the χT versus T plot.

Implementering og anvendelse af Activity-based Costing i Danmark

DEFF Research Database (Denmark)

Nielsen, Steen; Jakobsen, Morten

2007-01-01

.a. lønsomhed og virksomhedens indirekte eller faste omkostninger. Denne undersøgelses problemfelt tages derfor i at skabe en større indsigt i området, set i relation til det fremtidige potentiale og cost/benefit som implementering af ABC generelt forventes at have i danske virksomheder. Til sidst......Activity-Based Costing (ABC) er i dag ét af mest omtalte "moderne" økonomistyringsværktøjer blandt virksomheder og i forskerverdenen. Baseret på en en undersøgelse af 90 mellemstore og større danske fremstillingsvirksomheder præsenteres en Strukturel lignings model, som giver en helhedsforståelse...

Health Information in Somali (Af-Soomaali )

Science.gov (United States)

... and Wound Healing - Af-Soomaali (Somali) Bilingual PDF Health Information Translations Fasting Blood Sugar Test - Af-Soomaali (Somali) Bilingual PDF Health Information Translations GTT (Glucose Tolerance Test) - Af-Soomaali ( ...

Barrierer for ibrugtagning af videooptaget universitetsundervisning

DEFF Research Database (Denmark)

Rattleff, Pernille

2009-01-01

tilgængelige for studerende og andre interesserede. Der kan således iagttages nogle barrierer i forhold til ibrugtagning af videooptaget undervisning på universitetsniveau, idet underviserne for en umiddelbar betragtning tilsyneladende for det første har nogle forbehold af psykologisk, for det andet af...... begrebsafklaring, præsenterer forfatterne i denne artikel erfaringerne med tilbud af videostreamet og podcastet universitetsundervisning til studerende på Danmarks Pædagogiske Universitetsskole med henblik på en diskussion af de potentialer og især de barrierer, der kan iagttages i forhold til en mere systematisk...

Dialektisk billede af Benjamin

DEFF Research Database (Denmark)

Bøggild, Jacob

2004-01-01

Anmeldelse af Lars Brückner: "Løse blade. Læseren, kritikeren og fortælleren Walter Benjamin"......Anmeldelse af Lars Brückner: "Løse blade. Læseren, kritikeren og fortælleren Walter Benjamin"...

Strategi til udbredelse og udvikling af aquaponik

OpenAIRE

Bartholin, Aline; Nielsen, Per

2014-01-01

Denne rapport omhandler, hvorledes aquaponik i fremtiden skal udbredes med henblik på at produktionsmetoden bliver en del af den etablerede fødevaresektor. Ud fra et interview med PlanteLaboratoriet som er en del af foreningen Akvaponisk Selskab, samt observationer af en workshop afholdt af PlanteLaboratoriet forsøger vi at kortlægge den førte strategi til udbredelse og udvikling af aquaponik, samt undersøge hvorledes aquaponik som innovation bliver taget imod af mulige adoptere. Empirien sam...

Bekæmpelse af elmesyge

DEFF Research Database (Denmark)

Thomsen, Iben Margrete; Kristoffersen, Palle; Ravn, Hans Peter

2004-01-01

Elmesygen kom til Danmark i 1970’erne. Efter 1985 er udbredelsen gået stærkt, og elmesygen har været katastrofal for bestanden af bytræer og træer i landskabet. Bekæmpelse består fortrinsvis af sanering, dvs. fjernelse af syge og billeangrebne træer inden 1. maj....

Energiforbrug til opvarmning af bygninger

DEFF Research Database (Denmark)

Budde, J.; Pedersen, D.O.

Rapporten giver oplysninger om enhedsforbrug til varme og varmt brugsvand i forskellige typer af bygninger baseret på en analyse af et omfattende datamateriale. Formålet med undersøgelsen har været at ajourføre denne del af grundlaget for både landsdækkende og lokal varmeplanlægning i Danmark....

Udlejers godkendelse af ny erhvervslejer

DEFF Research Database (Denmark)

Munk-Hansen, Carsten

2009-01-01

I artiklen er taget udgangspunkt i den »klassiske« afståelsessituation, hvor en lejer vil afhænde den i de lejede lokaler drevne virksomhed, og lejeren har afståelsesret til en af udlejer godkendt lejer, uden at kravene til den ny lejer er specificeret. På baggrund af Vestre Landsrets dom af 9/2 ...

Dannelsen af den ansvarlige elev

DEFF Research Database (Denmark)

Larsen, Jesper

2010-01-01

Denne artikel handler om den del af lærergerningen, der har at gøre med udarbejdelse af elevplaner. Der tages udgangspunkt i en foucaultsk forståelse, hvor beskrivelsen af den enkelte elev udtrykker et særligt normativt ideal om, hvilke former for elevhed der er de ønskværdige i den danske...

Interlaboratory testing of porcine sera for antibodies to porcine circovirus type 2

DEFF Research Database (Denmark)

McNair, I.; Marshall, M.; McNeilly, F.

2004-01-01

A panel of 20 porcine sera was distributed to 5 laboratories across Europe and Canada. Each center was requested to test the sera for the presence of porcine circovirus type 2 antibodies using the routine assays, indirect immunofluorescence assay (IFA) and indirect immunoperoxidase monolayer assa...... than did IFA, and paraformaldehyde gave higher titers than did acetone or ethyl alcohol. This report highlights the need for standardized procedures and biologicals for this virus....

Didaktisk design af et pervasive learning game

DEFF Research Database (Denmark)

Oldenskov, Joakim; Westergren, Jens; Frank, Anders Bredahl

2010-01-01

Projektet er et studie i det teoretiske grundlag for, og en empirisk undersøgelse, af en prototype for et didaktisk design af et pervasive learning game. Der arbejdes overordnet med udviklingen af et didaktisk design af et læringsspil som kombinerer mobile teknologier, det fysiske rum og eleverne...... game med en analyse af legen og motivationen iboende spillet. I det læringsteoretiske felt inddrages og diskuteres forskellige tilgange til læring og pervasive games, som alle repræsenterer et socialkonstruktivistisk syn på læring. Det didaktiske design danner grundlag for et prototypedesign af et...... funktionalitet som en del af undervisningen. Det er afgørende at teknologien er pålidelig og er let at gå til, da det er kernefunktionen i denne type af spil. Legens rolle er vigtig for motivationen, men må ikke overtage de formelle krav til den undervisning, som spillet er del af. Det var entydigt omkring...

Vurdering af omfang og konsekvenser af sprøjtning og gødskning af § 3-beskyttede naturarealer

DEFF Research Database (Denmark)

Strandberg, Morten Tune; Bak, Jesper Leth; Bladt, Jesper Stentoft

2012-01-01

Det er overvejende de ferske enge, der gødskes og sprøjtes, hvorimod dette kun undtagelsesvist er konstateret på andre naturtyper som overdrev og heder m.m. De ferske enge er samtidig den mest udbredte § 3-naturtype. Behandling i form af gødskning og sprøjtning foregår anslået på op til 20 % af a...

På sporet af innovation

DEFF Research Database (Denmark)

Strandgaard, Lone Bak

organisationen blandt ledere og medarbejdere samt eksternt, uden for organisationen, hos dens kunder. Ph.d.-afhandlingen På sporet af innovation har som formål at bidrage til forståelse af, hvordan organisationers arbejde med digital selvbetjening kan fremmes, set i et innovationsteoretisk perspektiv. Foruden en...... indføring i innovationsteorien og dens udvikling belyser afhandlingens resultater hvordan at innovationspotentialet kan styrkes gennem organisatorisk åbenhed og samarbejde i alle dele af den innovative proces. Dette gælder ved både idéudvikling, realisering og spredning af nye digitale løsninger.......Flere og flere private og offentlige servicer digitaliseres, så kunder, borgere og virksomheder, i øget grad betjener sig selv. Digital selvbetjening kan potentielt effektivisere driften og skabe bedre kundeservice. Men processen har udfordringer, da den kan kræve omfattende omstilling internt i...

Dansk oversættelse af MiDAS, et redskab til assessment af musikterapi for personer med demens

DEFF Research Database (Denmark)

Ridder, Hanne Mette Ochsner; Lykkegaard, Chris; McDermott, Orii

2018-01-01

DAS explored which procedures is used for outcome measure translation and cultural adaptation, and based on this recommended a ten-step procedure. We applied this procedure in order to translate MiDAS and hereby develop the Danish MiDAS-DK. The Danish MiDAS version has been tested by more than 20 professionals......) musikterapeuten umiddelbart efter sessionen for at vurdere forandringer i selve sessionen. Der har været stor interesse for at anvende MiDAS i flere Europæiske lande, herunder Danmark. Derfor undersøgte udviklerne af MiDAS hvilke procedurer der anvendes til oversættelse og kulturel tilpasning af...... assessmentredskaber, og anbefalede på den baggrund en ti-trins procedure. Vi anvendte denne procedure til oversættelsen af MiDAS og således udviklingen af den danske MiDAS-DK. Den danske version af MiDAS er afprøvet af mere end 20 fagpersoner på baggrund af 296 udfyldte MiDAS-skemaer. Målet med artiklen er...

MR-AFS: a global hierarchical file-system

International Nuclear Information System (INIS)

Reuter, H.

2000-01-01

The next generation of fusion experiments will use object-oriented technology creating the need for world wide sharing of an underlying hierarchical file-system. The Andrew file system (AFS) is a well known and widely spread global distributed file-system. Multiple-resident-AFS (MR-AFS) combines the features of AFS with hierarchical storage management systems. Files in MR-AFS therefore may be migrated on secondary storage, such as roboted tape libraries. MR-AFS is in use at IPP for the current experiments and data originating from super-computer applications. Experiences and scalability issues are discussed

Raman af hvide pigmenter

DEFF Research Database (Denmark)

Reeler, Nini Elisabeth Abildgaard; Nielsen, Ole Faurskov; Sauer, Stephan P. A.

2013-01-01

Et samspil mellem kunst og kemi. I et samarbejde mellem Statens Museum for Kunst og Kemisk Institut på KU er Ramanspek-troskopi brugt til at definere sammensætningen af blandinger af blyhvidt og calcit i maleriers hvide pigmenter....

Midtvejsevaluering af EGA handlingsplanen

DEFF Research Database (Denmark)

Møller, Niels; Hasle, Peter; Christiansen, Jørgen Møller

Center for Alternativ Samfundsanalyse (CASA) og Institut for Teknologi og Samfund ved Danmarks Tekniske Universitet har gennemført denne midtvejsevaluering af arbejdsmarkedets parters handlingsplan mod reduktion af ensidigt gentaget arbejde. Undersøgelsen viser, at der er sket en betydelig...

A comprehensive computational model of sound transmission through the porcine lung.

Science.gov (United States)

Dai, Zoujun; Peng, Ying; Henry, Brian M; Mansy, Hansen A; Sandler, Richard H; Royston, Thomas J

2014-09-01

A comprehensive computational simulation model of sound transmission through the porcine lung is introduced and experimentally evaluated. This "subject-specific" model utilizes parenchymal and major airway geometry derived from x-ray CT images. The lung parenchyma is modeled as a poroviscoelastic material using Biot theory. A finite element (FE) mesh of the lung that includes airway detail is created and used in comsol FE software to simulate the vibroacoustic response of the lung to sound input at the trachea. The FE simulation model is validated by comparing simulation results to experimental measurements using scanning laser Doppler vibrometry on the surface of an excised, preserved lung. The FE model can also be used to calculate and visualize vibroacoustic pressure and motion inside the lung and its airways caused by the acoustic input. The effect of diffuse lung fibrosis and of a local tumor on the lung acoustic response is simulated and visualized using the FE model. In the future, this type of visualization can be compared and matched with experimentally obtained elastographic images to better quantify regional lung material properties to noninvasively diagnose and stage disease and response to treatment.

Does an interdisciplinary outpatient atrial fibrillation (AF) clinic affect the number of acute AF admissions?

DEFF Research Database (Denmark)

Frydensberg, Vivi Skibdal; Brandes, Axel

2018-01-01

AIMS AND OBJECTIVES: To investigate the impact of a multidisciplinary outpatient clinic for patients with atrial fibrillation (AF-clinic) on the number of acute hospitalizations for atrial fibrillation compared with usual outpatient care for this condition before the establishment of the AF-clinic....... BACKGROUND: Guidelines recommend a structured outpatient clinic follow-up in multidisciplinary teams for patients with atrial fibrillation due to fewer hospitalizations and death. However the evidence base is weak. DESIGN: The study is a retrospective cohort study including a total of 129 patients from...... the cardiac outpatient clinic at a large Danish University Hospital. The study population consisted of two groups: a usual care group before a dedicated AF-clinic was established (n=73) and the AF-clinic group (n=56). The primary endpoint was acute hospitalization for AF. METHODS: Cox regression was used...

A novel porcine model of ventilator-associated pneumonia caused by oropharyngeal challenge with Pseudomonas aeruginosa.

Science.gov (United States)

Li Bassi, Gianluigi; Rigol, Montserrat; Marti, Joan-Daniel; Saucedo, Lina; Ranzani, Otavio T; Roca, Ignasi; Cabanas, Maria; Muñoz, Laura; Giunta, Valeria; Luque, Nestor; Rinaudo, Mariano; Esperatti, Mariano; Fernandez-Barat, Laia; Ferrer, Miquel; Vila, Jordi; Ramirez, Jose; Torres, Antoni

2014-05-01

Animal models of ventilator-associated pneumonia (VAP) in primates, sheep, and pigs differ in the underlying pulmonary injury, etiology, bacterial inoculation methods, and time to onset. The most common ovine and porcine models do not reproduce the primary pathogenic mechanism of the disease, through the aspiration of oropharyngeal pathogens, or the most prevalent human etiology. Herein the authors characterize a novel porcine model of VAP due to aspiration of oropharyngeal secretions colonized by Pseudomonas aeruginosa. Ten healthy pigs were intubated, positioned in anti-Trendelenburg, and mechanically ventilated for 72 h. Three animals did not receive bacterial challenge, whereas in seven animals, a P. aeruginosa suspension was instilled into the oropharynx. Tracheal aspirates were cultured and respiratory mechanics were recorded. On autopsy, lobar samples were obtained to corroborate VAP through microbiological and histological studies. In animals not challenged, diverse bacterial colonization of the airways was found and monolobar VAP rarely developed. In animals with P. aeruginosa challenge, colonization of tracheal secretion increased up to 6.39 ± 0.34 log colony-forming unit (cfu)/ml (P VAP was confirmed in six of seven pigs, in 78% of the cases developed in the dependent lung segments (right medium and lower lobes, P = 0.032). The static respiratory system elastance worsened to 41.5 ± 5.8 cm H2O/l (P = 0.001). The authors devised a VAP model caused by aspiration of oropharyngeal P. aeruginosa, a frequent causative pathogen of human VAP. The model also overcomes the practical and legislative limitations associated with the use of primates. The authors' model could be employed to study pathophysiologic mechanisms, as well as novel diagnostic/preventive strategies.

Learning design – praktisk planlægning af et blended undervisningsforløb

DEFF Research Database (Denmark)

Hansen, Janne Saltoft

undervisningsplanlægning. Model og toolkit introduceres desuden generelt som planlægningsværktøj på Health til planlægning af blendede undervisningsforløb. På workshoppen præsenteres learning design-model og toolkit, samt de teoretiske rammer, som de valg, der er truffet i forbindelse med udviklingen, er baseret på......I 2011 vedtog Aarhus Universitet en politik for educational it, der indeholdt visioner for anskaffelse af et nyt learning management system (LMS), undervisningsudvikling og uddannelse af undervisere. Blackboard blev anskaffet, og implementering samt uddannelse af undervisere startede i slutningen...... udvidet kursus. For at eksplicitere og kvalitetssikre undervisningsplanlægningen med educational it blev det besluttet at binde undervisningen i Blackboard op på et learning design med en central learning design-model, som skulle lægge op til at skabe sammenhængende undervisning, understøtte aktiverende...

Lejere der bliver sat ud af deres bolig

DEFF Research Database (Denmark)

Christensen, Gunvor; Nielsen, Torben Heien

Antallet af lejere, der bliver sat ud af deres bolig, er steget i perioden 2002-06. I 2006 blev 2600 husstande, svarende til 0,1 pct. af samtlige husstande i Danmark, sat ud af deres bolig. Stigningen er især sket i de fem største byer: København, Århus, Odense, Ålborg og Esbjerg. I dette...... skal give viden om, hvilke årsager der er til, at nogle lejere bliver sat ud af deres bolig, og hvorfor der er sket en stigning i antallet af udsættelser. Den endelige afrapportering af undersøgelsen af udsættelser i Danmark vil ske i foråret 2008. Undersøgelsen er finansieret af Socialministeriet....

An in-depth comparison of the porcine, murine and human inflammasomes; lessons from the porcine genome and transcriptome.

Science.gov (United States)

Dawson, Harry D; Smith, Allen D; Chen, Celine; Urban, Joseph F

2017-04-01

Emerging evidence suggests that swine are a scientifically acceptable intermediate species between rodents and humans to model immune function relevant to humans. The swine genome has recently been sequenced and several preliminary structural and functional analysis of the porcine immunome have been published. Herein we provide an expanded in silico analysis using an improved assembly of the porcine transcriptome that provides an in depth analysis of genes that are related to inflammasomes, responses to Toll-like receptor ligands, and M1 macrophage polarization and Escherichia coli as a model organism. Comparisons of the expansion or contraction of orthologous gene families indicated more similar rates and classes of genes in humans and pigs than in mice; however several novel porcine or artiodactyl-specific paralogs or pseudogenes were identified. Conservation of homology and structural motifs of orthologs revealed that the overall similarity to human proteins was significantly higher for pigs compared to mouse. Despite these similarities, two out of four canonical inflammasome pathways, Absent in melanoma 2 (AIM2) and NLR family and CARD domain containing 4 (NLRC4), were found to be missing in pigs. Pig M1 Mφ polarization in response to interferon-γ (IFN-γ) and lipopolysaccharide (LPS) was assessed, via the transcriptome, using next generation sequencing. Our analysis revealed predominantly human-like responses however some, mouse-like responses were observed, as well as induction of numerous pig or artiodactyl-specific genes. This work supports using swine to model both human immunological and inflammatory responses to infection. However, caution must be exercised as pigs differ from humans in several fundamental pathways. Published by Elsevier B.V.

Registrering af biometriske og biologiske personoplysninger

DEFF Research Database (Denmark)

Blume, Peter Erik; Herrmann, Janne Rothmar

2009-01-01

Med udgangspunkt i menneskeretsdomstolens Marper dom drøftes i artiklen indretningen af dansk rets regler om registrering af fingeraftryk og dna-profil med tilhørende biologisk materiale, og om dommen nødvendiggør ændringer af disse regler....

Hærdeteknologi og Holdbarhed af Betonkonstruktioner

DEFF Research Database (Denmark)

Sørensen, Eigil V.

Kurset Hærdeteknologi og Holdbarhed af Betonkonstruktioner afholdes i 1. og 2. periode af forårssemesteret 2006. Kurset sigter på at give de studerende en grundlæggende forståelse af, hvorledes man designer og udfører holdbare betonkonstruktioner....

International fragmentation af produktionen og vertikal disintegration af styringsformer i produktionskæderne

DEFF Research Database (Denmark)

Refslund, Bjarke

Stigende international økonomisk integration har betydet en stigende fragmentering af produktionen, hvor store dele udføres i forskellige lande. Dertil kommer en stigende disintegration af vertikale styringsformer i produktions-værdikæder (Global Value Chains). Dette konferencepaper diskutere...

Identifikation af forkullet lig ved hjælp af ganeaftryk og koralnyresten

DEFF Research Database (Denmark)

Leth, Peter Mygind; Bindslev, Dorthe Arenholt; Kristensen, Ingrid Bayer

1997-01-01

En retstodontologisk undersøgelse af en dødfunden, uidentificeret person, bygger almindeligvis på en undersøgelse af tandsættet. Hvis personen er tandløs må andre identifikationsmetoder tages i anvendelse. Fingeraftryk er et almindelig kendt eksempel på en alternativ metode, som bygger på, at cri...

An Interface Transformation Strategy for AF-IPPS

Science.gov (United States)

2012-12-01

Oracle, or SAP . Like other AF and Department of Defense (DoD) ERP programs, AF-IPPS is expected to have 100+ interfaces with 90+ external trading partners...platform such as PeopleSoft, Oracle, or SAP . Like other AF and Department of Defense (DoD) ERP programs, AF-IPPS is expected to have 100...currently perform these functions. The core of AF-IPPS will be implemented using an Enterprise Resource Planning ( ERP ) platform such as PeopleSoft

Coaching af dit studieliv

DEFF Research Database (Denmark)

Hansen, Rasmus Thorning

2008-01-01

En generel beskrivelse af de problemer specialestuderende sidder med og hvorledes coaching kan hjælpe med at (gen)skabe motivation og fokus......En generel beskrivelse af de problemer specialestuderende sidder med og hvorledes coaching kan hjælpe med at (gen)skabe motivation og fokus...

Kvalitetsvurdering af sundhedsoplysende materiale

DEFF Research Database (Denmark)

Osler, Merete; Bech, Lars; Sybille, Bojlén

1990-01-01

Lægeforeningens Hygiejnekomités arbejdsgruppe vedrørende sundhedsoplysning har udarbejdet en "huskeliste" til brug for en kvalitetsvurdering af skriftligt sundhedsoplysende materiale......Lægeforeningens Hygiejnekomités arbejdsgruppe vedrørende sundhedsoplysning har udarbejdet en "huskeliste" til brug for en kvalitetsvurdering af skriftligt sundhedsoplysende materiale...

Implementering af evidensbaseret praksis

DEFF Research Database (Denmark)

Scheel, Linda Schumann; Tewes, Marianne; Petersen, Preben Ulrich

2015-01-01

Projektet omhandler implementering af den kliniske retningslinje om identifikation, forebyggelse og behandling af delirium – i denne sammenhæng relateret til hjertepatienter. Projektet foregår på to hospitaler i Hjertecentret på Rigshospitalet, et intensivt afsnit og et sengeafsnit, i tæt...

Implementering af evidensbaseret praksis

DEFF Research Database (Denmark)

Scheel, Linda Schumann; Hansen, Ida Rode

2014-01-01

Projektet omhandler implementering af den kliniske retningslinje om identifikation, forebyggelse og behandling af delirium – i denne sammenhæng relateret til hjertepatienter. Projektet foregår på to hospitaler i Hjertecentret på Rigshospitalet, et intensivt afsnit og et sengeafsnit, i tæt...

Magtens repræsentation af danskhed

DEFF Research Database (Denmark)

Madsen, Carsten

2016-01-01

Med udgangspunkt i Maurice Charlands begreb om konstitutiv retorik og Jacques Derridas dekonstruktion af repræsentationslogik foretager artiklen en kritisk analyse og fortolkning af repræsentationen af dansk nationalidentitet, sådan som den kommer til udtryk i Dronning Margrethes nytårstaler...

På kanten af ungdomslivet

DEFF Research Database (Denmark)

Sørensen, Niels Ulrik; Pless, Mette

2015-01-01

). Parallelt hermed fortælles moderne ungdomsliv (i ungdomssociologien) frem gennem ”…theoretical frameworks based on urban experiences which capture neither the lives of rural young people nor the spatial dimensions of the structures and cultures that make up contemporary youth.” (Farrugia 2013......, at yderområder er hårdt ramt af ungdomsarbejdsløshed (Bjørnsted & Andersen 2013), at områderne er præget af høj koncentration af unge med arbejderklassebaggrund (Ottosen et a 2010), mens de uddannelsesorienterede og ressourcestærke unge er tilbøjelige til at flytte væk og ind til byerne (Helve 2003). Og ser man...... stedstilknytning og stedbundne ressourcer. Teoretiske perspektiver, der peger på vigtigheden af en mere ”..spatialised youth sociology that is sensitive to macro-level processes producing different spaces, as well as the local, emplaced ways in which young people are responding to social changest hat shape...

For bestemmelse af drivhusgasudledning ved udtagning/Ekstensivering af landbrugsjorder på kulstofrige lavbundsjorder

DEFF Research Database (Denmark)

Gyldenkærne, Steen; Greve, Mogens Humlekrog

Sammenfatning: Rapporten beskriver hvordan man kan beregne ændringen i drivhusgasemissionen ved omlægning af landbrugsjord til naturarealer. Rapporten er primært rettet mod tilskudsordningen vedr. udtagning af organiske lavbundsjorde, men kan også anvendes i andre sammenhænge. Rapporten anvender de...

Omsætning af fast ejendom

DEFF Research Database (Denmark)

Edlund, Hans Henrik

Bogen er en systematisk gennemgang af lov om omsætning af fast ejendom, der regulerer ejendomsmægleres virke. Følgende emner er bl.a. behandlet: Hvem kan drive ejendomsmæglervirksomhed og ejendomsmæglernes pligter og ansvar.......Bogen er en systematisk gennemgang af lov om omsætning af fast ejendom, der regulerer ejendomsmægleres virke. Følgende emner er bl.a. behandlet: Hvem kan drive ejendomsmæglervirksomhed og ejendomsmæglernes pligter og ansvar....

Fermentering af korn øger fordøjeligheden af næringsstoffer

DEFF Research Database (Denmark)

Pedersen, Anni Øyan; Jørgensen, Henry; Bach Knudsen, Knud Erik

2010-01-01

foderblandingerne med ikke-fermentet korn var dog lavere end normalt, hvilket kan have påvirket resultaterne, så effekten af fermentering er større i dette forsøg, end hvad der normalt kan forventes. Kornet var groft formalet (ca. 30 % under 1 mm), hvilket kan have påvirket resultaterne. Ved finere formaling af...

Bilag til forskningsoversigt - Effekterne af Cooperative Learning

DEFF Research Database (Denmark)

Larsen, Lea Lund

Ved en opsamling af de empiriske studier vedrørende effekten af CL inden for voksenundervisningsfeltet ses en overvægt af studier, som rapporterer en positiv effekt af CL i forhold til de voksne lærendes faglige udbytte. En meta-analyse over 168 amerikanske undersøgelser på universitetsniveau vis...

Læring som improviseret udvikling af praksis

DEFF Research Database (Denmark)

Brinck, Lars

2010-01-01

Med afsæt i forfatterens fænomenografiske studie af amerikanske musikeres oplevelse af det at jamme diskuterer artiklen, hvorvidt jam-bandets spontant improviserende praksis kan give ny forståelse af rytmiske musikeres læring som et overordnet socialt fænomen. Ved at betragte jam-bandets praksis i...

Hvorfor lejere bliver sat ud af deres bolig

DEFF Research Database (Denmark)

Nielsen, Torben Heien; Christensen, Gunvor

Antallet af lejere, der bliver sat ud af deres bolig, er steget fra 1499 i 2002 til 2589 i 2006. Lejere kan blive sat ud af deres bolig, hvis de ikke betaler deres husleje til tiden, eller hvis de ikke overholder husordenen det sted de bor. Denne rapport beskriver årsager til, at lejere bliver sat...... ud af deres boliger og baggrunden for stigningen i antallet af udsættelser. desuden beskriver rapporten konsekvenser af en udsættelse og kommer med en række anbefalinger til indsatsområder. Af afgørende betydning for, at lejere bliver sat ud af deres bolig, er lav indkomst, lavt rådighedsbeløb, stor...

Enzymatisk omestring til produktion af biodiesel

DEFF Research Database (Denmark)

Fjerbæk, Lene

2007-01-01

  Biodiesel er i dag sammen med bioethanol et bud på, hvordan transportsektoren kan nedbringe sin netto CO2-emission til atmosfæren og lagrene af fossilt brændstof kan strækkes. På verdensplan forventes der en produktion af biodiesel på 7,9 mio. tons i 2007. Ved den industrielle fremstilling af...... biodiesel benyttes i dag kemiske katalysatorer såsom H2SO4, NaOH, MeONa eller KOH, der efterfølgende fjernes fra den producerede biodiesel med store mængder vand og derved produceres store mængder spildevand. Ved at benytte enzymer i processen kan man reducere mængden af spildevand, der skal renses. Enzymer...... benyttes ikke i de eksisterende processer, men det forventes, at udviklingen af processerne vil øge deres anvendelse i biodieselproduktion. I artiklen præsenteres fordele og ulemper ved anvendelse af enzymer til biodiesel produktion....

Anvendelse af genmodificerede planter

DEFF Research Database (Denmark)

Møller, F.

I rapporten vurderes mulighederne for at gennemføre velfærdsøkonomiske analyser af anvendelsen af genmodificerede planter. Der gøres rede for kravene til konse-kvensbeskrivelsen og for mulighederne for at prissætte såvel de markedsrelaterede som de ikke-markedsrelaterede konsekvenser. Mulighederne...

Fundering af mindre bygninger

DEFF Research Database (Denmark)

Larsen, J.; Ballisager, C.C.

Denne SBI-anvisning indeholder en gennemgang af de jordbunds- og grundvandsforhold, som er bestemmende for funderingen af en bygning. Specielt gennemgås de betingelser, der skal være opfyldt, for at man på forsvarlig måde kan fundere en mindre bygning i traditionel udformning. Publikationen...

Udvikling af vejledningspraksis i kurser for undervisere i brugen af blended learning-aktiviteter

DEFF Research Database (Denmark)

Stenalt, Maria Hvid; Wichmann-Hansen, Gitte; Rossen, Dorte Sidelmann

Hvert semester tilbyder Center for Undervisning og Læring (CUL) ved School of Business and Social Sciences ved Aarhus Universitet et 1-årig langt kompetenceudviklingsforløb i brugen af blended learning for lektorer og professorer. I løbet af denne periode skal deltagerne gennemføre et indledende...... blended learning-kursusforløb på 5 uger efterfulgt af en udviklings- og afprøvningsfase. Som afslutning skal deltagerne skrive en refleksionsrapport. Forløbet indebærer to vejledningsmøder med en eller flere af de undervisningsudviklere fra CUL, der er ansvarlige for forløbet: 1) Et individuelt...... vejledningsmøde, hvor deltagerne skal finde frem til, hvordan de vil eller kan omlægge deres undervisning til blended learning og 2) et kollektivt vejledningsmøde efter første udviklingsfase, hvor deltagerne modtager feedback på deres igangværende udviklingstiltag fra gruppemedlemmer og undervisningsudviklere. Et...

Comparison of Acute Thrombogenicity for Metallic and Polymeric Bioabsorbable Scaffolds: Magmaris Versus Absorb in a Porcine Arteriovenous Shunt Model.

Science.gov (United States)

Waksman, Ron; Lipinski, Michael J; Acampado, Eduardo; Cheng, Qi; Adams, Lila; Torii, Sho; Gai, Jiaxiang; Torguson, Rebecca; Hellinga, David M; Westman, Peter C; Joner, Michael; Zumstein, Philine; Kolodgie, Frank D; Virmani, Renu

2017-08-01

A comparison in acute thrombogenicity between the Magmaris sirolimus-eluting bioabsorbable magnesium scaffold and the Absorb bioresorbable vascular scaffold has not been performed. This study assessed acute thrombogenicity of Magmaris compared with Absorb and the Orsiro hybrid drug-eluting stent in a porcine arteriovenous shunt model. An ex vivo porcine carotid jugular arteriovenous shunt was established and connected to SYLGARD tubing containing the Magmaris, Absorb, and Orsiro scaffolds/stents and allowed to run in the shunt for a maximum of 1 hour. Twelve shunts (2 shunt runs per pig) were run comparing the 3 scaffolds in alternating order. Nested generalized linear mixed models were used to compare variables between scaffold groups while adjusting for variability between shunt runs. Confocal fluorescent microscopy costaining CD61/CD42b demonstrated that both Magmaris (3.0%) and Orsiro (4.6%) had less platelet coverage of the total scaffold compared with Absorb (21.8%). Scanning electron microscopy demonstrated significantly less thrombus deposition to Magmaris as a percentage of the total scaffold compared with Absorb (5.0% versus 16.1%, P =0.02). Magmaris had significantly less PM-1-positive neutrophil and CD14-positive monocyte adherence compared with both Orsiro and Absorb. Orsiro had significantly less monocyte deposition compared with Absorb. Despite a similar scaffold strut thickness, the Magmaris sirolimus-eluting bioabsorbable magnesium scaffold was significantly less thrombogenic compared with the Absorb bioresorbable vascular scaffold in an ex vivo porcine arteriovenous shunt model. Further studies are needed to determine whether the reduced thrombogenicity of Magmaris will result in reductions in major cardiovascular events. © 2017 American Heart Association, Inc.

Videoanalyse af social interaktion

DEFF Research Database (Denmark)

Igennem de sidste 40 år er videooptagelser af praksis blevet anvendt som et redskab i forskning af social interaktion inden for f.eks. læring, design, mediering, kommunikationstræning og i sundhedsvæsenet. Videoanalyse af social interaktion giver et stærkt metodisk, teoretisk og praktisk blik ind i...... videoanalyse og viser, hvordan man som forsker og studerende kan bruge videoanalyse til at undersøge social interaktion. Bogens temaer og spørgsmål udgør således en grundsten i arbejdet med videoanalyse på professions-, universitets- og forskeruddannelser såvel som i forskning mere generelt og omfatter: - de...

Fluorerede stoffer i mademballage af pap og papir

DEFF Research Database (Denmark)

Trier, Xenia; Cederberg, Tommy Licht; Jensen, Lisbeth Krüger

andre typer af madbokse. Prøverne er blevet analyseret for flere forskellige typer af fluorerede stoffer, der anvendes til at imprægnere disse typer af emballager, så de ikke suger fedt eller vand. I ingen af prøverne er der fundet indhold af fluorerede stoffer, der overstiger analysemetodens...

Characterization of serotonergic receptors in rabbit, porcine and human conjunctivae.

Science.gov (United States)

Turner, Helen C; Alvarez, Lawrence J; Candia, Oscar A; Bernstein, Audrey M

2003-10-01

To characterize the serotonin (5-HT) receptors linked to the modulation of adenylyl cyclase activity in rabbit, porcine and human conjunctivae. Serotonin receptor-subtype expression was examined using reverse transcription-polymerase chain reaction (RT-PCR) and receptor subtype-specific polyclonal antibodies for the immunofluorescent labeling of conjunctival cryosections. In addition, measurements of the effects of serotonergics on the short-circuit current (I(sc)) across rabbit and porcine conjunctivae were contrasted. RT-PCR assays indicated the expression of 5-HT(1B ) and 5-HT(1D) receptors, subtypes negatively coupled to adenylyl cyclase, in the rabbit conjunctiva. This approach also suggested the co-expression of 5-HT(1B), 5-HT(1D), 5-HT(1F), 5-HT(4) and 5-HT(7) mRNA's in the porcine conjunctiva, and 5-HT( 1D), 5-HT(1F) and 5-HT(7) in the human conjunctiva. Since the 5-HT(4) and 5-HT(7) receptors are positively linked to adenylyl cyclase, these results implied that the porcine and human tissues exhibited subtypes both positively and negatively linked to the enzyme. However, immunohistochemical observations, using currently available antibodies solely localized the 5-HT(7) moiety in the porcine and human epithelia, suggested that the 1B/1D forms may be minor elements. Consistent with this prospect, 5-HT was a stimulant of the transepithelial I(sc) across the porcine conjunctiva, an opposite response from earlier findings that demonstrated inhibitory effects by 5-HT on the rabbit I(sc), which are now explained by the localization of the 1B/1D receptors in the rabbit stratified epithelium. The 5-HT receptors expressed by mammalian conjunctivae are not identical. In terms of 5-HT receptor expression, the porcine tissue may be a more appropriate model for human, than is the rabbit, in that 5-HT may serve as a secretagogue in the human epithelium.

Aspekter af integrationsindsatsen i 6 kommuner

DEFF Research Database (Denmark)

Rosdahl, Anders

Dette arbejdspapir præsenterer resultaterne af en mindre kvalitativ undersøgelse af kommuners indsats for at bringe nyankomne flygtninge og indvandrere i beskæftigelse. Undersøgelsen er en opfølgning på en kvantitativ benchmarkanalyse, som er gennemført af Amternes og Kommunernes Forskningsinstit...

Modelanalyser af mobilitet og miljø

DEFF Research Database (Denmark)

Christensen, Linda; Gudmundsson, Henrik

Begrebet bæredygtig transport diskuteres og ved hjælp af persontrafikmodellen ALTRANS belyses mulighederne for at fremme bæredygtig udvikling gennem en række scenarier. Desuden præsenteres en mere detaljeret analyse af ændringer i CO2-belastningen ved forbedring af den kollektive trafiks servicen...

Toward Development of Pluripotent Porcine Stem Cells by Road Mapping Early Embryonic Development

DEFF Research Database (Denmark)

Petkov, Stoyan; Freude, Kristine; Mashayekhi-Nezamabadi, Kaveh

2017-01-01

The lack in production of bona fide porcine pluripotent stem cells has definitely been hampered by a lack of research into porcine embryo development. Embryonic development in mammals is the extraordinary transition of a single-celled fertilized zygote into a complex fetus, which occurs...... in the uterus of the maternal adult during the early stages of gestation. Biomedical pig models could serve as genetic backgrounds for establishment of embryonic stem cells (ESCs) or other pluripotent stem cells (such as iPSC), which may be used to model and study diseases in vitro. This chapter provides...... insight into the current knowledge of pluripotent states in the developing pig embryo and the current status in establishment of bona fide porcine ESC (pESC) and piPSCs. It reflects the potential causes underlying the difficulty in establishing pluripotent stem cells and reviews recent data on global...

Boligboblen pustes op af bankerne

DEFF Research Database (Denmark)

Ravn, Ib

2017-01-01

Den aktuelle boligboble blæses i vejret af bankerne, der gennem opkøb af realkreditobligationer skaber nye penge og dermed presser boligpriserne i vejret - en kreditfinansieret bolig- eller finansboble....

Domesticeringen af Ford i Danmark:Den kulturelle tilegnelse af Ford og fordismen, 1920-1940

OpenAIRE

Wagner, Michael

2009-01-01

Det amerikanske automobilfirma Ford Motor Company med hovedkvarter i Detroit begyndte at drive forretning i Danmark omkring 1906. De første aktiviteter blev konsolideret samme år ved udnævnelsen af motorforhandleren F. Bülow & Co. til salgsagent i Danmark og importør af Ford automobiler til det skandinaviske marked. Dermed havde Ford tidligt fået et stærkt fodfæste i Nordeuropa, som udviklingen i salget af Ford autobiler de næste årtier til fulde skulle demonstrere. Ford Motor Company er ...

Sygeplejestuderendes brug af portfolio i klinisk undervisning

DEFF Research Database (Denmark)

Hjorth, Anne Charlotte Overgaard; Bruhn, Helle

2014-01-01

Brugen af portfolio var mangelfuld, men et udviklingsprojekt i samarbejde med den kommunale sygepleje motiverede både sygeplejestuderende og kliniske vejledere til at anvende læringsdelen af portfolio aktivt.......Brugen af portfolio var mangelfuld, men et udviklingsprojekt i samarbejde med den kommunale sygepleje motiverede både sygeplejestuderende og kliniske vejledere til at anvende læringsdelen af portfolio aktivt....

Vurdering af effekten af en vindmøllepark ved Overgaard på forekomsten af fugle i EF-fuglebeskyttelsesområde nr. 15

DEFF Research Database (Denmark)

Clausen, P.; Larsen, J. K.

forekomsten af en række arter der enten yngler eller raster inden for EF-fuglebeskyttelsesområdet, og er omfattet af EF-fuglebeskyttelsesdirektivets bilag I. Det drejer sig om arterne skarv Phalacrocorax carbo sinensis, blå kærhøg Circus cyaneus, hjejle Pluvialis apricaria, brushane Philomachus pugnax, klyde...

Selektiv avl af racehunde og -katte

DEFF Research Database (Denmark)

Sandøe, Peter; Meyer, Iben Helene Coakley; Fredholm, Merete

2014-01-01

Dette kapitel ser nærmere på de velfærdsmæssige problemstillinger, der opstår som følge af måden, hvorpå racehunde og -katte påvirkes gennem selektiv avl. Først beskrives, hvordan avlen af racehunde og -katte er organiseret. Dernæst gøres der rede for de dyrevelfærdsmæssige konsekvenser heraf....... Selektiv avl af hunde og katte tjener til at vedligeholde en stor racemæssig diversitet. De racemæssige forskelle gør det muligt for mennesker at vælge især hunde, der har nogle egenskaber, som passer til individuelle behov og ønsker. Desværre er der også en række bivirkninger for de pågældende dyrs...... problemer, som knytter sig til selektiv avl af racehunde og -katte, gennemgås og diskuteres mulige praktiske tiltag, som kan trække udviklingen i retning af at reducere forekomsten af disse problemer....

Quantification of intervertebral displacement with a novel MRI-based modeling technique: Assessing measurement bias and reliability with a porcine spine model.

Science.gov (United States)

Mahato, Niladri K; Montuelle, Stephane; Goubeaux, Craig; Cotton, John; Williams, Susan; Thomas, James; Clark, Brian C

2017-05-01

The purpose of this study was to develop a novel magnetic resonance imaging (MRI)-based modeling technique for measuring intervertebral displacements. Here, we present the measurement bias and reliability of the developmental work using a porcine spine model. Porcine lumbar vertebral segments were fitted in a custom-built apparatus placed within an externally calibrated imaging volume of an open-MRI scanner. The apparatus allowed movement of the vertebrae through pre-assigned magnitudes of sagittal and coronal translation and rotation. The induced displacements were imaged with static (T 1 ) and fast dynamic (2D HYCE S) pulse sequences. These images were imported into animation software, in which these images formed a background 'scene'. Three-dimensional models of vertebrae were created using static axial scans from the specimen and then transferred into the animation environment. In the animation environment, the user manually moved the models (rotoscoping) to perform model-to-'scene' matching to fit the models to their image silhouettes and assigned anatomical joint axes to the motion-segments. The animation protocol quantified the experimental translation and rotation displacements between the vertebral models. Accuracy of the technique was calculated as 'bias' using a linear mixed effects model, average percentage error and root mean square errors. Between-session reliability was examined by computing intra-class correlation coefficients (ICC) and the coefficient of variations (CV). For translation trials, a constant bias (β 0 ) of 0.35 (±0.11) mm was detected for the 2D HYCE S sequence (p=0.01). The model did not demonstrate significant additional bias with each mm increase in experimental translation (β 1 Displacement=0.01mm; p=0.69). Using the T 1 sequence for the same assessments did not significantly change the bias (p>0.05). ICC values for the T 1 and 2D HYCE S pulse sequences were 0.98 and 0.97, respectively. For rotation trials, a constant bias (�

Realisering af Vision 2020

DEFF Research Database (Denmark)

Bertelsen, Niels Haldor; Hansen, Ernst Jan de Place

Repræsentanter for byggesektoren har på 11 dialogmøder drøftet Erhvervs- og Byggestyrelsens "Vision 2020 - Byggeri med mening". Drøftelserne førte til formulering af en lang række initiativforslag til realisering af visionen. Den mest centrale udfordring bliver at reducere fejl og mangler i...... byggeriet. Branchen lægger også vægt på, at styringen af Vision 2020s reaisering sker i byggesektoren. Initiativforslagene er i rapporten samlet under 3 hovedområder. Det første hovedområde lægger vægt på bygningerne, brugerbehov og det globale samfund. Det andet omhandler processen og leverancesystemet...

The rolling-circle melting-pot model for porcine circovirus DNA replication

Science.gov (United States)

A stem-loop structure, formed by a pair of inverted repeats during DNA replication, is a conserved feature at the origin of DNA replication (Ori) among plant and animal viruses, bacteriophages and plasmids that replicate their genomes via the rolling-circle replication (RCR) mechanism. Porcine circo...

Konceptuelt design og udvikling af Intergomus

DEFF Research Database (Denmark)

Ellebæk, P.; Pedersen, T.; Stendal, Karsten

1999-01-01

Denne rapport gennemgår udviklingsforløbet af et træningsapparat til genoptræning af læderede fingre. Rapporten omhandler kun den mekaniske del af udstyret. Opgaven er blevet løst i samarbejde med Ergoterapien på Køge Amtssygehus, Procare, Centret forAvanceret Teknologi og flere afdelinger på RIS...

Offentliggørelse af kursrelevante oplysninger

DEFF Research Database (Denmark)

Werlauff, Erik; Schaumburg-Müller, Peer

2013-01-01

Artiklen analyserer, særligt på baggrund af EU-Domstolens dom af 28. juni 2012 i C-19/11, Daimler AG, hvilken betydning en række mellemliggende trin, der fører frem til en kursrelevant og offentliggørelsespligtig begivenhed, har for tidspunktet for indtrædelsen af pligten til offentliggørelse. De...

Regulering af jødiske kroppe

DEFF Research Database (Denmark)

Schleicher, Marianne

2014-01-01

på teori dels af Michel Foucault om, hvordan kultur sikrer sin egen overlevelse ved at indsamle viden om bl.a. kroppe med henblik på at regulere brugen af dem, dels af Judith Butler, der bl.a. forklarer, hvordan kultur trods alt er åben for såkaldt subversion, idet det bidrager til at udvide og...

En litteraturbaseret gennemgang af mulige velfærdsmæssig betydning af brugen af længerevarende smertelindring til kalve efter afhorning

DEFF Research Database (Denmark)

Herskin, Mette S.; Nielsen, Bodil Højlund

2018-01-01

Dette er et udvidet sammendrag af vidensyntesen. Store dele af vidensyntesen er under review til et videnskabeligt tidsskrift, og kan derfor ikke offentliggøres elektronisk før denne proces er afsluttet. Den fulde rapport kan fremsendes pr. mail. Hvis du gerne vil have den tilsendt, så send en mail...

Kuratering af Samtidskunst

DEFF Research Database (Denmark)

Kuratering af Samtidskunst er den første bogudgivelse i Danmark, der fokuserer på kuratering som fag og praksis. Kuratering spiller en stadig større rolle i samtidskunsten. Bogen er en introduktion til skiftende forståelser af udstillings- og kuratorpraksisser og deres udfordringer, indkredset ge...... Kofod Olsen, Teresa Gleadowe, Helle Ryberg, Kirse Junge-Stevnsborg, Malene Natascha Ratcliffe, Pelin Uran, Jacob Fabricius, Lotte Juul Petersen, Jacob Lillemose, Solvej Helweg Ovesen, Frederikke Hansen & Tone Olaf Nielsen (kuratorisk aktion) og Temporary Services....

Evaluering af kollegial supervision

DEFF Research Database (Denmark)

Petersen, Anne Line Bjerre Folsgaard; Bager, Lene Tortzen; Jørgensen, Mette Eg

2015-01-01

Videoen er en evaluering af arbejdet med en metodisk tilgang til kollegial supervision på VIA Ergoterapeutuddannelsen gennem et par år. Evalueringen sætter fokus på selve metoden, der er anvendt til kollegial supervision. Derudover er der fokus på erfaringer og udbytte af at arbejde systematisk med...... kollegial supervision blandt undervisere på VIA Ergoterapeutuddannelsen....

Effekter af koordineret hjerneskaderehabilitering. Et systematisk litteraturreview

DEFF Research Database (Denmark)

Glintborg, Chalotte; Hansen, Tia G. B.

beskrevet. Der er ingen klar dansk definition af koordination. Begrebet bliver ofte brugt inkonsistent, og koordination er meget forskelligt tilrettelagt. Derfor kan man ikke generelt dokumentere, om koordinatorfunktioner er en sikker vej til forbedring af komplekse rehabiliteringsforløb. Sundhedsstyrelsen......Forskningsprojekt udvikler ny definition af begrebet koordination Som et led i forskningsprojektet ”Bio-psyko-sociale effekter af koordineret hjerneskaderehabilitering” er der udviklet en ny definition af koordinationsbegrebet. Forskningsprojektet ”Bio-psyko-sociale effekter af koordineret...... senhjerneskadeområdet – initiativer, der skal fremme en sammenhængende og helhedsorienteret rehabiliteringsindsats. Men hvad dækker begrebet koordination egentlig over? Det har et nyt forskningsprojekt set på og kommer med et bud på en ny definition. Dansk definition mangler Indholdet i koordination har været upræcist...

Mass Customization og Postponement - strategier og modeller for integreret produktion og distribution af kundespecifikke produkter

DEFF Research Database (Denmark)

Michelsen, Aage U.; Janus, Pagh

2002-01-01

Mass Customization er et produktionskoncept, der søger at kombinere masseproduktion og enkeltstyksproduktion på en sådan måde, at det er muligt at "masseproducere" kundespecifikke produkter. Postponement er i denne forbindelse et centralt begreb, idet det vedrører placeringen af dekoblingspunktet...... ("customer order decoupling point"), dvs. placeringen af det lager, hvorfra efterfølgende produktion og distribution er styret af kundespecifikke produkter. I artiklen fokuseres på, at hvis produktion og distribution ikke opfattes som to sekventielle systemer, men som ét integreret system, så opstår der en...

Erfaringer fra nedrivning af boligblok i Rødbyhavn

DEFF Research Database (Denmark)

Eriksen, Søren Skibstrup; Hansen, Klaus; Krogh, Hanne

Denne meddelelse indeholder en beskrivelse af nedrivningen af et betonelementbyggeri. Der er foretaget en registrering af energiforbrug i forbindelse med nedrivningen og bortskaffelsen samt en registrering af de miljøbelastende stoffer i bygningsaffaldet. Inden bebyggelsen blev revet ned, blev de...

Overvågning af karplanter i Suserup Skov

DEFF Research Database (Denmark)

Wind, P.

1999-01-01

Vejler, i alt ca. 5.500 ha. Feltstationen blev oprettet i 1978, og siden har observatørerne registreret fuglelivet i Vejlerne og de omgivende arealer foruden vandstand, saltholdighed og drift af området. Tipperne ligger i den sydlige del af Ringkøbing Fjord. Fuglelivet blev fredet i 1898. Reservatet blev...... en samlet fredning af Vadehavet, omfatter øen Langli på 80 ha og vandarealer på i alt ca. 8.000 ha. Øen består af klitter og salte strandenge. Vandområdet er præget af tidevand, hvor vidtstrakte sand- og mudderflader og spredte muslingebanker er synlige ved lavvande. Vorsø ligger i Horsens Fjord...... saltholdighed i Ringkøbing Fjord bevirker, at vandplanter vokser dårligt eller forsvinder. Vandplanter udgør en væsentlig del af føden for visse andefugle og kan derfor have stor betydning for antallet af rastende fugle. Der blev registreret lave antal af knortegå, gråand, pibeand og krikand. Af blishøne blev...

Exchange bias mechanism in FM/FM/AF spin valve systems in the presence of random unidirectional anisotropy field at the AF interface: The role played by the interface roughness due to randomness

Science.gov (United States)

Yüksel, Yusuf

2018-05-01

We propose an atomistic model and present Monte Carlo simulation results regarding the influence of FM/AF interface structure on the hysteresis mechanism and exchange bias behavior for a spin valve type FM/FM/AF magnetic junction. We simulate perfectly flat and roughened interface structures both with uncompensated interfacial AF moments. In order to simulate rough interface effect, we introduce the concept of random exchange anisotropy field induced at the interface, and acting on the interface AF spins. Our results yield that different types of the random field distributions of anisotropy field may lead to different behavior of exchange bias.

Miljørigtig ombygning af Mallinggård

DEFF Research Database (Denmark)

Hansen, Ernst Jan de Place

2002-01-01

Resume af rapport om den byggetekniske anvendelse af alternativ isolering ved en ombygning af Mallinggårds lade til undervisningslokaler, udarbejdet af Bent Møller fra Rudolf Steiner Skolen i Skanderborg under Energistyrelsens udviklingsprogram "Miljø- og arbejdsmiljøvenlig isolering"...

Et nationalt studie af bankrøveri

DEFF Research Database (Denmark)

Hansen, Maj; Elklit, Ask

11,1 % (n = 41) af ASD og seks måneder efter røveriet led 6,2 % (n = 23) af PTSD. Begge prævalenser var begrænset af kravet om undgåelsessymptomer (ASD uden undgåelse: 14 %, PTSD uden undgåelse: 17,8 %), men ASD diagnosen var især begrænset af kravet om dissociationssymptomer (ASD uden dissociation......: 26,7 %). Hierarkisk regressionsanalyse viste, at 51 % af variansen i PTSD sværhedsgrad blev forklaret af peritraumatisk dissociation, ASD sværhedsgrad, emotionel coping og negative tanker om egen person og evner. ASD sværhedsgrad var den stærkeste prædiktorer for PTSD sværhedsgrad og forudsagde alene...... diagnoser. Særligt ASD sværhedsgrad og negative tanker om egen person og evner synes at kunne forudsige PTSD sværhedsgrad efter bankrøveri. Det anbefales derfor, at ”Acute Stress Disorder Scale” anvendes til at screene for PTSD efter bankrøveri. Fremtidig forskning anbefales at fokusere på udvikling af...

Typebestemmelse af de danske M. bovis stammer

DEFF Research Database (Denmark)

Kokotovic, Branko

Mycoplasma bovis er en af de mest betydningsfulde mykoplasma i kvægbruget på verdensplan. Bakterien findes oftest i forbindelse med mastitis, lunge- og ledbetændelse, men andre kliniske manifestationer kan også forekomme. Der findes ikke præcise, nyere beregninger over konsekvenser af M. bovis...... relateret sygdomme, men det anses, at infektionen hvert år globalt set pålægger en økonomisk byrde til kvægbruget i en multimillion skala. M. bovis blev for første gang påvist i Danmark i 1981 og gennem 1980’erne har bakterien forårsaget store udbrud af mastitis. I de følgende årtier blev bakterien isoleret...... sporadisk fra mange forskellige kvægbesætninger, men på trods af en tilsyneladende stigende forekomst i 1990’erne, blev der ikke registret omfattende sundhedsmæssige problemer som følge af M. bovis smitte. Siden 2011 er M. bovis dog kommet i fokus igen på grund af alvorlige udbrud af især ledbetændelse, men...

E1AF degradation by a ubiquitin-proteasome pathway

International Nuclear Information System (INIS)

Takahashi, Akiko; Higashino, Fumihiro; Aoyagi, Mariko; Yoshida, Koichi; Itoh, Miyuki; Kobayashi, Masanobu; Totsuka, Yasunori; Kohgo, Takao; Shindoh, Masanobu

2005-01-01

E1AF is a member of the ETS family of transcription factors. In mammary tumors, overexpression of E1AF is associated with tumorigenesis, but E1AF protein has hardly been detected and its degradation mechanism is not yet clear. Here we show that E1AF protein is stabilized by treatment with the 26S protease inhibitor MG132. We found that E1AF was modified by ubiquitin through the C-terminal region and ubiquitinated E1AF aggregated in nuclear dots, and that the inhibition of proteasome-activated transcription from E1AF target promoters. These results suggest that E1AF is degraded via the ubiquitin-proteasome pathway, which has some effect on E1AF function

7 CFR 1230.18 - Porcine animal.

Science.gov (United States)

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Porcine animal. 1230.18 Section 1230.18 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... animal. Porcine animal means a swine, that is raised as (a) a feeder pig, that is, a young pig sold to...

Depressive patienters oplevelse af at modtage ECT

DEFF Research Database (Denmark)

Jensen, Christine Damsbo

Dette studie handler om, hvordan det opleves for den voksne depressive patient at gennemgå et ECT-behandlingsforløb (electroconvulsive therapy). Formålet med projektet er dels at undersøge, hvordan processen omkring ECT-behandlingen udspiller sig og dels at undersøge patienternes oplevelse......, reaktioner og tanker omkring ECT-behandlingen. Studiet bygger på den eksisterende viden om patienters oplevelse af ECT. Metoden er feltforskning med deltagende observation og etnografisk interview. Dette suppleres af to spørgeskemaer. Der blev inkluderet 8 patienter i undersøgelsen. Analysen udspringer af...... konstruktivistisk epistemiologisk vinkel. Ved en teoristyret analyse fremkom 2 overordnede temaer: Anspændthed og tryghed. Disse temaer blev behandlet i en procesorienteret forståelse af ECT-forløbet. Denne proces bestod af: Betingelser, interaktion og konsekvenser. Diskussionen er en kritisk diskussion af fund og...

Overvågning af orkidéer 2000

DEFF Research Database (Denmark)

Wind, P.

resultaterne af orkidéovervågningen (Wind 2001). Hertil kommer præsentationen af projektet og dets resultater efter forløbet af de første tre år (Løjtnant 1991a) og en bearbejdning af projektets resultater for årene 1987-1995 (Wind 1997). I nærværende rapport undersøges skov-hullæbe for en eventuel sammenhæng...... mellem skudanlæggelsen udtrykt ved antallet af blomstrende og vegetative skud i et givet år og nedbørsmængder i august og september året før. Det er en udbredt antagelse at der er en sammenhæng mellem skudanlæggelse og mængden af nedbør det foregående år. Undersøgelser af skov-hullæbe populationer i...... truet og sårbar samt de to arter der er omfattet af Habitatdirektivets bilag 2. I 2000 kan det i forhold til 1999 konstateres at der i 15 bestande er optalt et øget antal skud. I 11 bestande er der noteret en nedgang i antallet af skud sammenlignet med optællingerne i 1999. Af disse er der 2 be...

Ekstraktion af æteriske olier fra oregano

DEFF Research Database (Denmark)

Ellitsgaard Jakobsen, Jens; Ankerfeldt, Dorte; Jensen, Morten Busch

2010-01-01

med keratin - Ammoniakforurening reduceres med ny teknologi - Genvinding og genanvendelse af ammoniak fra spildevand og husdyrgødning - Ginseng - en virkningsfuld medicinplante - Ekstraktion af æteriske olier fra oregano "Biotek" er udviklet med udgangspunkt i originalartikler udarbejdet af forskere...

Børn af veteraner

DEFF Research Database (Denmark)

Frederiksen, Signe; Lausten, Mette

Siden starten af 1990’erne har Danmark sendt over 30.000 soldater og andet personel på internationale missioner – langt de fleste til Eks-Jugoslavien, Afghanistan og Irak. Denne undersøgelse handler om de børn, hvis fædre har været udsendt på militære missioner i udlandet. For hvordan håndterer...... spørgsmål er fokus for undersøgelsen. Undersøgelsen bygger på en spørgeskemaundersøgelse blandt børn af veteraner på henholdsvis 7, 11 og 15 år. Undersøgelsen er gennemført for og i samarbejde med Veterancentrets Videncenter, som er en del af Forsvarsministeriets Personalestyrelse....

Effects of 32P radioactive stents on in-stent restenosis in a double stent injury model of the porcine coronary arteries

International Nuclear Information System (INIS)

Kim, Han-Soo; Chan, Rosanna C.; Kollum, Marc; Au, Arthur; Tio, Fermin O.; Yazdi, Hamid A.; Ajani, Andrew E.; Waksman, Ron

2001-01-01

Background: The major limitation of coronary stenting remains in-stent restenosis, due to the development of neointimal proliferation. Radioactive stents have demonstrated the ability to reduce this proliferation in the healthy nonatherosclerotic porcine animal model. However, inhibition of tissue proliferation in the in-stent restenotic lesion in a porcine model is not well characterized. The objective of this study was to examine the efficacy and safety of the 32 P radioactive stent for the treatment of in-stent restenosis in a double stent injury model of the porcine coronaries. Methods and Materials: Eighteen coronary arteries in 9 pigs underwent nonradioactive stent (8 mm in length) implantation. Thirty days after the initial stent implantation, a 32 P radioactive stent (18 mm in length) with an activity of 0 and 18 μCi was implanted to cover the initial stent. The swine were killed 30 days after the second stent implantation. Histomorphometric analysis was performed for vessel area (VA), stent strut area (SSA), intimal area (IA), and lumen area (LA). Results: Injury scores, VA, SSA, and LA were similar among the control and radiated groups. Neointimal formation was significantly reduced after placement of radioactive stents as compared to control in both the overlapped (0.93±0.12 vs. 1.31±0.51 mm 2 , p 2 , p 32 P radioactive stents may be safe and effective in reducing neointimal formation leading to in-stent restenosis. Longer follow-up will be required to examine whether these positive findings can be maintained

Experimental reproduction of postweaning multisystemic wasting syndrome (PMWS) in pigs in Sweden and Denmark with a Swedish isolate of porcine circovirus type 2

DEFF Research Database (Denmark)

Hasslung, F.; Wallgren, P.; Hansen, Anne-Sofie Ladekjær

2005-01-01

An experimental model using 3-day-old snatch-farrowed colostrum-deprived piglets co-infected with porcine circovirus type 2 (PCV2) and porcine parvovirus (PPV) is at present one of the best methods to study factors affecting development of postweaning multisystemic wasting syndrome (PMWS). A Swed......An experimental model using 3-day-old snatch-farrowed colostrum-deprived piglets co-infected with porcine circovirus type 2 (PCV2) and porcine parvovirus (PPV) is at present one of the best methods to study factors affecting development of postweaning multisystemic wasting syndrome (PMWS...

Characterization of the porcine TOR1A gene: The first step towards generation of a pig model for dystonia

DEFF Research Database (Denmark)

Henriksen, Carina; Madsen, Lone Bruhn; Bendixen, Christian

2009-01-01

. The TOR1A gene was demonstrated to be localized on porcine chromosome 1. Single nucleotide polymorphism (SNP) analysis revealed several SNPs in the porcine TOR1A gene, both in the coding region and also in the 3′ UTR region. Overexpression of mutant (Δ∆E303-304) porcine TorsinA in neuroblastoma cells...

Tællinger af vandfugle i Danmark 1998-99

DEFF Research Database (Denmark)

Pihl, S.; Laubek, B.

1999-01-01

Denne rapport præsenterer Miljø- og Energiministeriets seks feltstationer med beskrivelse af deres historie, naturforhold og overvågningsprogram. Resultaterne af fugleobservationer i 1997 beskrives i sammenhæng med tidligere års udvikling. Desuden præsenteres resultater af tre undersøgelser af he...

Om den sociale og symbolske funktion af uddannelsesevalueringer

DEFF Research Database (Denmark)

Nørholm, Morten

Ph.d.-afhandlingen handler om evalueringer, om evalueringer af uddannelse med et fokus og perspektiv som er begrænset til en afdækning af forhold om evalueringer, til en formulering af en teori om evalueringer. I afhandlingens analyser og diskussioner anvendes teorier af Émile Durkheim, Pierre...

Porcine cadaver iris model for iris heating during corneal surgery with a femtosecond laser

Science.gov (United States)

Sun, Hui; Fan, Zhongwei; Wang, Jiang; Yan, Ying; Juhasz, Tibor; Kurtz, Ron

2015-03-01

Multiple femtosecond lasers have now been cleared for use for ophthalmic surgery, including for creation of corneal flaps in LASIK surgery. Preliminary study indicated that during typical surgical use, laser energy may pass beyond the cornea with potential effects on the iris. As a model for laser exposure of the iris during femtosecond corneal surgery, we simulated the temperature rise in porcine cadaver iris during direct illumination by the femtosecond laser. Additionally, ex-vivo iris heating due to femtosecond laser irradiation was measured with an infrared thermal camera (Fluke corp. Everett, WA) as a validation of the simulation.

Delegation af lovgivningsmagt: risiko for ringere regulering?

DEFF Research Database (Denmark)

Pedersen, Morten Jarlbæk; Christensen, Geert Laier; Ravn, Jacob

2016-01-01

kortsigtetede, politiske incitamenter. Samtidig underlægges delegation ikke samme grad af kvalitetskontrolmekanismer som almindelig lovgivning; dette kunne ellers kompensere for den mere politiserede delegation. En øget brug af bekendtgørelser kan derfor betyde en stigende risiko for regulering af lavere...

Biotransformering af pyren i havbørsteormen Nereis virens

DEFF Research Database (Denmark)

Jørgensen, Anne; Giessing, Anders; Juul Rasmussen, Lene

føden og ved overførsel over epithelet. Graden af systemisk akkumulering af PAHer afhænger af optaget men ligeledes af effektiviteten af biotransformering i den pågældende organisme. N. virens er kendt for effektivt at biotransformere PAHer. I nærværende undersøgelse blev biotransformering undersøgt med......-glucuronid er den fase II metabolit der primært produceres ved biotransformering af pyren. Dette indikerer at de vigtigste enzymer i biotransformering af pyren i N. virens er CYP enzymer og glucuronosyl transferase enzymer....

Putative biomarkers for evaluating antibiotic treatment: an experimental model of porcine Actinobacillus pleuropneumoniae infection

DEFF Research Database (Denmark)

Lauritzen, B.; Lykkesfeldt, J.; Skaanild, M.T.

2003-01-01

Biomarkers of infection were screened for their possible role as evaluators of antibiotic treatment in an aerosol infection model of porcine pneumonia caused by Actinobacillus pleuropneumoniae (Ap). Following infection of 12 pigs, clinical signs of pneumonia developed within 20 h, whereafter...... antibiotic treatment of acute Ap-infection ill pigs. The present model provides a valuable tool in the evaluation of antibiotic treatments, offering the advantage of clinical and pathological examinations combined with the use of biochemical infection markers....... recovered clinically within 24h after treatment, whereas tiamulin-treated animals remained clinically ill until the end of the study, 48 h after treatment. A similar Picture was seen for the biomarkers of infection. During the infection period, plasma C-reactive protein (CRP), interleukin-6 and haptoglobin...

Danskernes brug af nyhedsmedier 2018

DEFF Research Database (Denmark)

Schrøder, Kim Christian; Ørsten, Mark; Eberholst, Mads Kæmsgaard

Hvis der er ét begreb, der i 2018 er blevet slået fast med syvtommersøm i den offentlige debat verden over, så er det ’fake news’! Bekymringen over fænomenet er vokset eksplosivt, samtidig med at det er blevet mere og mere uklart, hvad ’fake news’ egentlig dækker over. I dette års analyse af...... brugen af nyhedsmedier sætter Reuters Institute Digital News Report, og også denne specialrapport om Danmark, analytisk fokus på ’fake news’ og kaster lys over borgernes opfattelse af fænomenet på en måde, så vi får nuancerne i det komplekse begreb med. Et andet emne, der er blevet diskuteret...... for fake news og egne oplevelser af fake news, og danskernes viden om, hvordan nyheder bliver lavet (nyheds-literacy)....

Kontrol af konventionelle våben i Europa

DEFF Research Database (Denmark)

Hoffmann, Rune; Kierulf, John; Pradhan-Blach, Flemming

Rapporten indledes med en historisk gennemgang af tilblivelsen og udviklingen af Traktat om Konventionelle Væbnede Styrker i Europa (CFE), som blev iværksat af NATO og det tidligere Warszawapagten i 1973. Traktaten fastsætter et loft for antallet af kampvogne, pansrede kampkøretøjer, artilleripje...

Kommentarer til love og ændringer af love

DEFF Research Database (Denmark)

Møgelvang-Hansen, Peter

2002-01-01

folkeoplysende foreningsarbejde og Folkeuniversitetet (folkeoplysningsloven) og ophævelse af lov om daghøjskoler m.v. og lov om den fri ungdomduddannelse samt ændring af lov om refusion af udgifter til uddannelse i forbindelse med aktivering m.m. samt ændring af forskellige love (Reform for daghøjskolerne...

Porcine cluster of differentiation (CD) Markers 2017 Update

Science.gov (United States)

Pigs are a major source of food worldwide; preventing and treating their infectious diseases is essential, requiring a thorough understanding of porcine immunity. The use of pigs as models for human physiology is a growing area; progress in this area has been limited because the immune toolkit is no...

Bakteriesensor til on-line identifikation/bestemmelse af mikrobiel drikkevandskvalitet

DEFF Research Database (Denmark)

Bentien, Anders; Tilsted Mogensen, Claus; Østergaard, Else-Marie

Over det meste af verden defineres den mikrobielle drikkevandskvalitet primært på baggrund af grænseværdier for E. coli, coliforme bakterier og kimtal. Anvendelsen af den totale bakteriekoncentration til bestemmelse af mikrobiel drikkevandskvalitet er (af historiske årsager) ikke udbredt og primært...

Gastrin-releasing peptide in the porcine pancreas

DEFF Research Database (Denmark)

Holst, J J; Poulsen, Steen Seier

1987-01-01

to consist of one main form, namely the 27-amino acid peptide originally extracted from porcine stomach, and small amounts of a C-terminal fragment identical with the C-terminal 10-amino acid peptide. Gastrin-releasing peptide-like immunoreactivity released from the isolated perfused porcine pancreas during...... electrical vagal stimulation was shown by gel filtration to consist of the same two forms. By use of immunocytochemical techniques employing an antiserum directed against its N terminus, GRP was localized to varicose nerve fibers in close association with the exocrine tissue of the porcine pancreas...... in particular. Some fibers were found penetrating into pancreatic islets also. Immunoreactive nerve cell bodies as well as fibers were found within intrapancreatic ganglia. The potency of GRP in stimulating exocrine as well as endocrine secretion from the porcine pancreas, its presence in close contact...

Genlyden af et nej

DEFF Research Database (Denmark)

Östman, Lars

2011-01-01

Europa og den vestlige verden er ramt af en økonomisk krise. Det er en krise, der er anderledes, end den vi har været vant til fx fra 1980'erne.......Europa og den vestlige verden er ramt af en økonomisk krise. Det er en krise, der er anderledes, end den vi har været vant til fx fra 1980'erne....

Comparative Analysis of the Regulatory T Cells Dynamics in Peripheral Blood in Human and Porcine Polytrauma

Directory of Open Access Journals (Sweden)

Rafael Serve

2018-03-01

Full Text Available BackgroundSeverely injured patients experience substantial immunological stress in the aftermath of traumatic insult, which often results in systemic immune dysregulation. Regulatory T cells (Treg play a key role in the suppression of the immune response and in the maintenance of immunological homeostasis. Little is known about their presence and dynamics in blood after trauma, and nothing is known about Treg in the porcine polytrauma model. Here, we assessed different subsets of Treg in trauma patients (TP and compared those to either healthy volunteers (HV or data from porcine polytrauma.MethodsPeripheral blood was withdrawn from 20 TP with injury severity score (ISS ≥16 at the admittance to the emergency department (ED, and subsequently on day 1 and at day 3. Ten HV were included as controls (ctrl. The porcine polytrauma model consisted of a femur fracture, liver laceration, lung contusion, and hemorrhagic shock resulting in an ISS of 27. After polytrauma, the animals underwent resuscitation and surgical fracture fixation. Blood samples were withdrawn before and immediately after trauma, 24 and 72 h later. Different subsets of Treg, CD4+CD25+, CD4+CD25+FoxP3+, CD4+CD25+CD127−, and CD4+CD25+CD127−FoxP3+ were characterized by flow cytometry.ResultsAbsolute cell counts of leukocytes were significantly increasing after trauma, and again decreasing in the follow-up in human and porcine samples. The proportion of human Treg in the peripheral blood of TP admitted to the ED was lower when compared to HV. Their numbers did not recover until 72 h after trauma. Comparable data were found for all subsets. The situation in the porcine trauma model was comparable with the clinical data. In porcine peripheral blood before trauma, we could identify Treg with the typical immunophenotype (CD4+CD25+CD127−, which were virtually absent immediately after trauma. Similar to the human situation, most of these cells expressed FoxP3, as assessed by

Optisk scanning af spørgeskemaer

DEFF Research Database (Denmark)

Paulsen, Aksel

/ 1000 felter= 0.370 (95% CI: 0.160-0.729), (p= 0.020)). Der var ingen statistisk forskel mellem optisk scanning (fejl/ 1000 felter= 0.046 (95% CI: 0.001-0.258)) og manuel dobbelt indtastning (p=1.000). Konklusioner: Optisk scanning er et godt alternativ til manuel dobbelt indtastning for spørgeskema med......Formål og baggrund: Patient rapporterede outcomes i form af spørgeskemaer bruges i øgende grad i sundhedssektoren både i klinisk praksis og forskning. Ofte bruges spørgeskemaer i papirformat. Manuel dobbelt indtastning er defineret som guld standard for overføring af data til et elektronisk format......, men processen er tidkrævende. Optisk scanning af spørgeskemaer med automatisk registrering af svar kan være et alternativ, men videre validering af metoden er nødvendig. Design: 200 patienter blev tilfældigt udvalgt fra en kohorte på 5777 patienter der tidligere havde svaret på to forskellige...

En analyse af potentialet for salg af fødevarer gennem Internettet i Danmark og England

DEFF Research Database (Denmark)

Ramus, Kim Bjarne

2001-01-01

I Kim Ramus ph.d.-projekt gennemføres en undersøgelse af danske og britiske forbrugeres holdninger og præferencer til handel med fødevarer på Internettet, hvor formålet er at finde potentialet for salg af fødevarer online. Internettet er ganske udbredt i begge lande, men England er dog et godt st...... stykke foran Danmark med supermarkedskæden Tesco i spidsen, når det gælder salg af fødevarer. I Danmark er der endnu ingen landsdækkende kæder, som for alvor er gået i gang med at sælge fødevarer online.......I Kim Ramus ph.d.-projekt gennemføres en undersøgelse af danske og britiske forbrugeres holdninger og præferencer til handel med fødevarer på Internettet, hvor formålet er at finde potentialet for salg af fødevarer online. Internettet er ganske udbredt i begge lande, men England er dog et godt...

Nye ledelsesudfordringer i forebyggelse af stress i videnarbejdet

DEFF Research Database (Denmark)

Andersen, Vibeke; Ipsen, Christine; Mogensen, Mette

arbejdssituation og muligheder for forebyggelse af arbejdsrelateret stress i vidensarbejdet. – Et kvalitaivt studie af arbejdsrelateret stress i vidensarbejdet. Lyngby, The Technical University of Denmark. Kärreman, Dan, Stefan Svenningson and Mats Alvesson (2003): The Return of the Machine Bureaucracy? Management......Afsættet for projektet ”Forebyggelse af stress i videnarbejdet – mellem begejstring og belastning” er, at der er behov for en ny forståelse af stress i det moderne arbejdsliv, for at kunne arbejde med stressforebyggelse blandt videnarbejdere. I dette paper tager vi udgangspunkt i begrebet...... mangetydighed/ambiguity som en karakteristik af videnarbejdet, da det stiller nogle helt nye udfordringer til hvordan ledelse af videnarbejdet skal foregå, når målet er at forebygge arbejdsrelateret stress. Vi karakteriserer videnarbejdet som mangetydigt og som Alvesson (2004) påpeger, udgør dilemmaerne i...

Nyttigt bidrag til professionalisering af revisionsudvalg

DEFF Research Database (Denmark)

Thomsen, Steen

2009-01-01

Anmeldelse af: KPMG. 2009, Audit Committee Institute. Revisionsudvalg i Praksis. 2. reviderede udgave.......Anmeldelse af: KPMG. 2009, Audit Committee Institute. Revisionsudvalg i Praksis. 2. reviderede udgave....

DVD anmeldelse - En Verden af Lyd og Musik

DEFF Research Database (Denmark)

Holck, Ulla

2006-01-01

Anmeldelse af DVD-udgivelse af Claus Bang (2005): En Verden af Lyd og Musik. Musikterapi for døve, hørehæmmede og multihandicappede børn og unge. Al tale med undertekst eller tegnsprogstolkning. DVD-boks med 3 stk. dobbeltlag DVD beregnet til afspilning på PC. Udgivet af Musikterapi-foreningen En...

En kvantitativ metode til analyse af radio

Directory of Open Access Journals (Sweden)

Christine Lejre

2014-06-01

Full Text Available I den danske såvel som den internationale radiolitteratur er bud på metoder til analyse af radiomediet sparsomme. Det skyldes formentlig, at radiomediet er svært at analysere, fordi det er et medie, der ikke er visualiseret i form af billeder eller understøttet af printet tekst. Denne artikel har til formål at beskrive en ny kvantitativ metode til analyse af radio, der tager særligt hensyn til radiomediets modalitet – lyd struktureret som et lineært forløb i tid. Metoden understøtter dermed både radiomediet som et medie i tid og som et blindt medie. Metoden er udviklet i forbindelse med en komparativ analyse af kulturprogrammer på P1 og Radio24syv lavet for Danmarks Radio. Artiklen peger på, at metoden er velegnet til analyse af ikke kun radio, men også andre medieplatforme samt forskellige journalistiske stofområder.

Degradation of AF1Q by chaperone-mediated autophagy

International Nuclear Information System (INIS)

Li, Peng; Ji, Min; Lu, Fei; Zhang, Jingru; Li, Huanjie; Cui, Taixing; Li Wang, Xing; Tang, Dongqi; Ji, Chunyan

2014-01-01

AF1Q, a mixed lineage leukemia gene fusion partner, is identified as a poor prognostic biomarker for pediatric acute myeloid leukemia (AML), adult AML with normal cytogenetic and adult myelodysplastic syndrome. AF1Q is highly regulated during hematopoietic progenitor differentiation and development but its regulatory mechanism has not been defined clearly. In the present study, we used pharmacological and genetic approaches to influence chaperone-mediated autophagy (CMA) and explored the degradation mechanism of AF1Q. Pharmacological inhibitors of lysosomal degradation, such as chloroquine, increased AF1Q levels, whereas activators of CMA, including 6-aminonicotinamide and nutrient starvation, decreased AF1Q levels. AF1Q interacts with HSPA8 and LAMP-2A, which are core components of the CMA machinery. Knockdown of HSPA8 or LAMP-2A increased AF1Q protein levels, whereas overexpression showed the opposite effect. Using an amino acid deletion AF1Q mutation plasmid, we identified that AF1Q had a KFERQ-like motif which was recognized by HSPA8 for CMA-dependent proteolysis. In conclusion, we demonstrate for the first time that AF1Q can be degraded in lysosomes by CMA. - Highlights: • Chaperone-mediated autophagy (CMA) is involved in the degradation of AF1Q. • Macroautophagy does not contribute to the AF1Q degradation. • AF1Q has a KFERQ-like motif that is recognized by CMA core components

Degradation of AF1Q by chaperone-mediated autophagy

Energy Technology Data Exchange (ETDEWEB)

Li, Peng; Ji, Min; Lu, Fei; Zhang, Jingru [Department of Hematology, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital, Shandong University, Jinan 250012 (China); Li, Huanjie; Cui, Taixing; Li Wang, Xing [Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital, Shandong University, Jinan 250012 (China); Tang, Dongqi, E-mail: tangdq@sdu.edu.cn [Research Center for Cell Therapy, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital, Shandong University, Jinan 250012 (China); Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan 250033 (China); Ji, Chunyan, E-mail: jichunyan@sdu.edu.cn [Department of Hematology, Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital, Shandong University, Jinan 250012 (China)

2014-09-10

AF1Q, a mixed lineage leukemia gene fusion partner, is identified as a poor prognostic biomarker for pediatric acute myeloid leukemia (AML), adult AML with normal cytogenetic and adult myelodysplastic syndrome. AF1Q is highly regulated during hematopoietic progenitor differentiation and development but its regulatory mechanism has not been defined clearly. In the present study, we used pharmacological and genetic approaches to influence chaperone-mediated autophagy (CMA) and explored the degradation mechanism of AF1Q. Pharmacological inhibitors of lysosomal degradation, such as chloroquine, increased AF1Q levels, whereas activators of CMA, including 6-aminonicotinamide and nutrient starvation, decreased AF1Q levels. AF1Q interacts with HSPA8 and LAMP-2A, which are core components of the CMA machinery. Knockdown of HSPA8 or LAMP-2A increased AF1Q protein levels, whereas overexpression showed the opposite effect. Using an amino acid deletion AF1Q mutation plasmid, we identified that AF1Q had a KFERQ-like motif which was recognized by HSPA8 for CMA-dependent proteolysis. In conclusion, we demonstrate for the first time that AF1Q can be degraded in lysosomes by CMA. - Highlights: • Chaperone-mediated autophagy (CMA) is involved in the degradation of AF1Q. • Macroautophagy does not contribute to the AF1Q degradation. • AF1Q has a KFERQ-like motif that is recognized by CMA core components.

Pressetørring af parketstave - Simulationsmodel

DEFF Research Database (Denmark)

Poulsen, Niels Kjølstad; Sørensen, Mads Peter; Pinholt, Peter

2004-01-01

Nærværende rapport indgår som en del af det arbejde der er udført under BIPS centeret. Rapporten vedrører modelleringsarbejdet med Junckers pressetørringsproces. Det er målet at opstille en termodynamisk og mekanisk model for parketstavene under pressetørring med henblik på at forklare og om muligt...

Produktudvikling af fødevarer med Quality Function Deployment

DEFF Research Database (Denmark)

Bech, Anne C.

1998-01-01

Produktudvikling af fødevarer stiller stadig større krav til virksomhedernes kompetencer. QFD filosofien kan bidrage til at skabe en fælles forståelse af grundlaget for udvikling af nye produkter. Udgangspunktet er en forståelse af forbrugernes behov i relation til virksomhedens muligheder...... konkurrentprodukter. Efterfølgende faser handler om at generere forskellige mulige løsninger, inden der træffes valg af løsningsalternativ. Anvendelse af QFD modellen for produktudvikling betyder, at forbrugernes behov er centrale i hele produktudviklingsforløbet, samt at det udviklede produkt sigter mod at opfylde...... væsentlige identificerede behov set fra forbrugernes synspunkt. At virksomheden ser implementering af QFD som en investering, medarbejdernes commitment og målrettet projekt, er væsentlig for en succesfuld implementering af QFD til produktudvikling. QFD tankegangen illustreres med et eksempel på udvikling af...

Innovation af innovation

DEFF Research Database (Denmark)

Harste, Gorm

2009-01-01

, at innovation af innovationen forsøges gennemført på en måde, hvor tiden kræves at forholde sig til sin egen tidslighed i form af fremtid, nutid, fortid og ikke mindst i form af samtidighed. I tiden skal vi iagttage, hvordan vi iagttager tiden. Vi dobbelt-koder tiden på samme måde, som forskning forsker i...... organisationssystemerne. De to typer systemer kan noget helt bestemt med fænomenet tid. De kan synkronisere. Analyseres organisationssystemer ser vi, imidlertid at innovation kræver ro. Stærkt innovative systemer er militærsystemet og kunstsystemet, der også inddrages, og hvor vi ser paradokset mellem innovation og...... involution. Tid er med et medium og ikke et lufttomt rum. Tid er end ikke en gasart, men udgør et solidt fluidum, som samfundet bader i og flyder i, konstant i bevægelse. Reformer forudsætter former, og innovation forudsætter involution. Kun sådan muliggøres evolution....

Aminopeptidase-N-independent entry of porcine epidemic diarrhea virus into Vero or porcine small intestine epithelial cells.

Science.gov (United States)

Ji, Chun-Miao; Wang, Bin; Zhou, Jiyong; Huang, Yao-Wei

2018-04-01

A monkey cell line Vero (ATCC CCL-81) is commonly used for porcine epidemic diarrhea virus (PEDV) propagation in vitro. However, it is still controversial whether the porcine aminopeptidase N (pAPN) counterpart on Vero cells (Vero-APN) confers PEDV entry. We found that endogenous expression of Vero-APN was undetectable in the mRNA and the protein levels in Vero cells. We cloned the partial Vero-APN gene (3340-bp) containing exons 1 to 9 from cellular DNA and subsequently generated two APN-knockout Vero cell lines by CRISPR/Cas9 approach. PEDV infection of two APN-knockout Vero cells had the same efficiency as the Vero cells with or without neuraminidase treatment. A Vero cells stably expressing pAPN did not increase PEDV production. SiRNA-knockdown of pAPN in porcine jejunum epithelial cells had no effects on PEDV infection. The results suggest that there exists an additional cellular receptor on Vero or porcine jejunal cells independent of APN for PEDV entry. Copyright © 2018 Elsevier Inc. All rights reserved.

I Frans af Assisis fodspor

DEFF Research Database (Denmark)

Bonde, Lisbeth

2015-01-01

En fodrejse fra Assisi til Rom i Frans af Assisis fodspor sætter gang i tankerne og giver anledning til nye ideer......En fodrejse fra Assisi til Rom i Frans af Assisis fodspor sætter gang i tankerne og giver anledning til nye ideer...

Anbefalinger til organisering af uddannelserne

DEFF Research Database (Denmark)

Andersen, Lars; Andersen, Lars Døvling; Hornemann, Birte C.

2009-01-01

Notatet sammenfatter de diskussioner, som det af fakulteterne nedsatte udvalg vedrørende nye undervisnings- og eksamensformer har haft, og de anbefalinger til en omlægning af vores uddannelsessystem, som udvalget har valgt at give. Notatet er samtidig en begyndelse på en erfaringsudveksling om ny...

Regulation of Porcine Hepatic Cytochrome P450 — Implication for Boar Taint

Directory of Open Access Journals (Sweden)

Martin Krøyer Rasmussen

2014-09-01

Full Text Available Cytochrome P450 (CYP450 is the major family of enzymes involved in the metabolism of several xenobiotic and endogenous compounds. Among substrates for CYP450 is the tryptophan metabolite skatole (3-methylindole, one of the major contributors to the off-odour associated with boar-tainted meat. The accumulation of skatole in pigs is highly dependent on the hepatic clearance by CYP450s. In recent years, the porcine CYP450 has attracted attention both in relation to meat quality and as a potential model for human CYP450. The molecular regulation of CYP450 mRNA expression is controlled by several nuclear receptors and transcription factors that are targets for numerous endogenously and exogenously produced agonists and antagonists. Moreover, CYP450 expression and activity are affected by factors such as age, gender and feeding. The regulation of porcine CYP450 has been suggested to have more similarities with human CYP450 than other animal models, including rodents. This article reviews the available data on porcine hepatic CYP450s and its implications for boar taint.

Registrering og Benchmarking af Modus 2 Forskning

DEFF Research Database (Denmark)

Duus, Henrik Johannsen; Jørgensen, Jens E.

2004-01-01

Fremvæksten af modus 2 forskning stiller krav om en revurdering af højere læreanstalters evaluerings- og meriteringspraksis. Den endimensionale prioritering af modus 1 forskning såvel som dennes ligeså endimensionale evaluering via publikationer må afvises som utidssvarende. Som en konsekvens her...

NIR: optimerer produktionen af gammeldags modnede sild

DEFF Research Database (Denmark)

Svensson, T.; Bro, Rasmus; Nielsen, Henrik Hauch

2005-01-01

Måling med nærinfrarødt (NIR) lys er et godt supplement til de nuværende metoder til at følge modningen af sild saltede i tønder. Det viser resultaterne af et forskningsprojekt udført i samarbejde mellem Lykkeberg A/S, Danmarks Fiskeriundersøgelser og Den Kgl Veterinær- og Landbohøjskole. Ved hjælp...... af avanceret matematik er det nemt og hurtigt at bestemme modningsgraden af sild direkte fra en NIR måling....

In vitro porcine blood-brain barrier model for permeability studies: pCEL-X software pKa(FLUX) method for aqueous boundary layer correction and detailed data analysis.

Science.gov (United States)

Yusof, Siti R; Avdeef, Alex; Abbott, N Joan

2014-12-18

In vitro blood-brain barrier (BBB) models from primary brain endothelial cells can closely resemble the in vivo BBB, offering valuable models to assay BBB functions and to screen potential central nervous system drugs. We have recently developed an in vitro BBB model using primary porcine brain endothelial cells. The model shows expression of tight junction proteins and high transendothelial electrical resistance, evidence for a restrictive paracellular pathway. Validation studies using small drug-like compounds demonstrated functional uptake and efflux transporters, showing the suitability of the model to assay drug permeability. However, one limitation of in vitro model permeability measurement is the presence of the aqueous boundary layer (ABL) resulting from inefficient stirring during the permeability assay. The ABL can be a rate-limiting step in permeation, particularly for lipophilic compounds, causing underestimation of the permeability. If the ABL effect is ignored, the permeability measured in vitro will not reflect the permeability in vivo. To address the issue, we explored the combination of in vitro permeability measurement using our porcine model with the pKa(FLUX) method in pCEL-X software to correct for the ABL effect and allow a detailed analysis of in vitro (transendothelial) permeability data, Papp. Published Papp using porcine models generated by our group and other groups are also analyzed. From the Papp, intrinsic transcellular permeability (P0) is derived by simultaneous refinement using a weighted nonlinear regression, taking into account permeability through the ABL, paracellular permeability and filter restrictions on permeation. The in vitro P0 derived for 22 compounds (35 measurements) showed good correlation with P0 derived from in situ brain perfusion data (r(2)=0.61). The analysis also gave evidence for carrier-mediated uptake of naloxone, propranolol and vinblastine. The combination of the in vitro porcine model and the software

U2AF1 mutations alter splice site recognition in hematological malignancies.

Science.gov (United States)

Ilagan, Janine O; Ramakrishnan, Aravind; Hayes, Brian; Murphy, Michele E; Zebari, Ahmad S; Bradley, Philip; Bradley, Robert K

2015-01-01

Whole-exome sequencing studies have identified common mutations affecting genes encoding components of the RNA splicing machinery in hematological malignancies. Here, we sought to determine how mutations affecting the 3' splice site recognition factor U2AF1 alter its normal role in RNA splicing. We find that U2AF1 mutations influence the similarity of splicing programs in leukemias, but do not give rise to widespread splicing failure. U2AF1 mutations cause differential splicing of hundreds of genes, affecting biological pathways such as DNA methylation (DNMT3B), X chromosome inactivation (H2AFY), the DNA damage response (ATR, FANCA), and apoptosis (CASP8). We show that U2AF1 mutations alter the preferred 3' splice site motif in patients, in cell culture, and in vitro. Mutations affecting the first and second zinc fingers give rise to different alterations in splice site preference and largely distinct downstream splicing programs. These allele-specific effects are consistent with a computationally predicted model of U2AF1 in complex with RNA. Our findings suggest that U2AF1 mutations contribute to pathogenesis by causing quantitative changes in splicing that affect diverse cellular pathways, and give insight into the normal function of U2AF1's zinc finger domains. © 2015 Ilagan et al.; Published by Cold Spring Harbor Laboratory Press.

Undersøgelse af udviklingen i maksimale vandstande i den østligste del af Limfjorden Aalborg – Hals

DEFF Research Database (Denmark)

Burcharth, Hans Falk; Røge, Mads Sønderstrup

Udviklingen i maksimalvandstande i den veslige del af Limfjorden har påkaldt sig stor interesse i forbindelse med diskussionen om stigende oversvømmelsesfrekvens relateret til en eventuel klimaændring. Formålet med nærværende undersøgelse er at belyse udviklingen i maksimalvandstande i den østlig...... østligste del af Limfjorden på strækningen fra Aalborg til Hals, en strækning hvor der er lavtliggende områder på begge sider af fjorden. Målinger af højvandstande i Aalborg er foretaget siden 1944 og i Hals siden april 2002....

Preliminary study of laser welding for aortic dissection in a porcine model using a diode laser with indocyanine green.

Science.gov (United States)

Fujita, Masanori; Morimoto, Yuji; Ohmori, Sayaka; Usami, Noriko; Arai, Tsunenori; Maehara, Tadaaki; Kikuchi, Makoto

2003-01-01

The objective of this study was to determine whether a dissected aorta could be welded by a diode laser with a solder using an in vitro porcine aortic dissection model. Porcine aortic strips were dissected into two flaps and the dissected faces were immersed in a solution of indocyanine green. The two flaps were pressed at 0.2 kg/cm2 with contact between the two immersed faces. The pressed flaps were irradiated with a diode laser (810 nm) at intensities of 170-425 W/cm2 for 8 seconds. The welded flaps were studied by light microscopy and the adhesive strengths were measured. The irradiated flaps were successfully welded. The breaking stress, the maximum stress recorded in a stress-strain curve, increased with increase in irradiation intensity up to 396 W/cm2 (2.7 x 10(2) mmHg) and decreased when the intensity reached 425 W/cm2. In the specimen irradiated at 396 W/cm2, the welded faces showed continuous fusion of elastin layers, while some voids were seen between the welded faces in the specimen irradiated at 425 W/cm2. The dissected porcine aortas were successfully welded using a laser with solder. The results suggest that the welded aorta can bear physiological blood pressure. Copyright 2003 Wiley-Liss, Inc.

Midtvejsrapport – Udvikling, test og validering af QTI-spørgsmål i en dansk gymnasiekontekst

DEFF Research Database (Denmark)

Lund, Lea; Lund, Rolf Lyneborg

Rapporten omhandler specifikt udviklingen af en dansk version af spørgsmålene i ’Questionnaire on Teachers Interaction’ (QTI), som er et element af projektet ”Relationskompetence og Klasseledelse i gymnasiet” (ROK). QTI er et empirisk velafprøvet og udbredt spørgeskema til elever, som giver et blik...... Utrecht Universitet til stadighed ændrer på begrebsbetegnelserne i deres modeller for MITB (The Model for Interpersonal Teacher Behavior), som er grundlaget for QTI. Det er vores håb med denne rapports gennemsigtighed omkring spørgsmålsudviklingen og fremlæggelsen af alle data og den endeligt statistisk...

Functional recovery after experimental RPE debridement, mfERG studies in a porcine model

DEFF Research Database (Denmark)

Sørensen, Nina Buus; Lassota, Nathan; Kyhn, Maria Voss

2013-01-01

BACKGROUND: The correlation between histologically identified regeneration of retinal pigment epithelium (RPE) and functional outcome measured by multifocal electroretinography (mfERG) following surgical debridement is examined in a porcine model. In humans, visual acuity is reduced in diseases......, and by brushing the Bruch's membrane with a silicone catheter. Immediately following surgery (baseline) and after 2 and 6 weeks respectively, the animals were examined by mfERG, fundus photographs (FPs), fluorescein angiograms (FAs), and histopathology. RESULTS: The mfERG P1 amplitude was decreased 2 weeks (T2....... CONCLUSION: This is the first study to show that the histological regeneration of hypopigmented RPE correlates to a return of the retinal function, measured by mfERG....

Arbejdsmiljøet ved udbud af renovationsarbejde

DEFF Research Database (Denmark)

Busck, Ole Gunni

Forfatteren gennemførte 2003-2005 en undersøgelse for Branchearbejdsmiljådet for transport af danske kommuners praksis i forbindelse med udbud og udlicitering af renovationsopgaver. Formålet var at kortlægge og analysere erfaringerne med varetagelse af arbejdsmiljøhensyn i kommunernes udbud og dr...

Bone marrow mesenchymal stem cells from infants with MLL-AF4+ acute leukemia harbor and express the MLL-AF4 fusion gene

Science.gov (United States)

Catalina, Purificación; Rodríguez, René; Melen, Gustavo J.; Bueno, Clara; Arriero, Mar; García-Sánchez, Félix; Lassaletta, Alvaro; García-Sanz, Ramón

2009-01-01

MLL-AF4 fusion is a hallmark genetic abnormality in infant B-acute lymphoblastic leukemia (B-ALL) known to arise in utero. The cellular origin of leukemic fusion genes during human development is difficult to ascertain. The bone marrow (BM) microenvironment plays an important role in the pathogenesis of several hematological malignances. BM mesenchymal stem cells (BM-MSC) from 38 children diagnosed with cytogenetically different acute leukemias were screened for leukemic fusion genes. Fusion genes were absent in BM-MSCs of childhood leukemias carrying TEL-AML1, BCR-ABL, AML1-ETO, MLL-AF9, MLL-AF10, MLL-ENL or hyperdiploidy. However, MLL-AF4 was detected and expressed in BM-MSCs from all cases of MLL-AF4+ B-ALL. Unlike leukemic blasts, MLL-AF4+ BM-MSCs did not display monoclonal Ig gene rearrangements. Endogenous or ectopic expression of MLL-AF4 exerted no effect on MSC culture homeostasis. These findings suggest that MSCs may be in part tumor-related, highlighting an unrecognized role of the BM milieu on the pathogenesis of MLL-AF4+ B-ALL. MLL-AF4 itself is not sufficient for MSC transformation and the expression of MLL-AF4 in MSCs is compatible with a mesenchymal phenotype, suggesting a differential impact in the hematopoietic system and mesenchyme. The absence of monoclonal rearrangements in MLL-AF4+ BM-MSCs precludes the possibility of cellular plasticity or de-differentiation of B-ALL blasts and suggests that MLL-AF4 might arise in a population of prehematopoietic precursors. PMID:19995953

Diagnostic investigation of porcine periweaning failure-to-thrive syndrome: lack of compelling evidence linking to common porcine pathogens.

Science.gov (United States)

Huang, Yanyun; Gauvreau, Henry; Harding, John

2012-01-01

Porcine periweaning failure-to-thrive syndrome (PFTS), an increasingly recognized syndrome in the swine industry of North America, is characterized by the anorexia of nursery pigs noticeable within 1 week of weaning, and progressive loss of body condition and lethargy during the next 1-2 weeks. Morbidity caused by PFTS is moderate, but case fatality is high. The etiology of PFTS is presently unknown and may include infectious agent(s), noninfectious factors, or both. PFTS was identified in a high health status farm with good management in early 2007. A diagnostic investigation was undertaken to identify the pathological lesions of, and infectious agents associated with, pigs demonstrating typical clinical signs. Affected (PFTS-SICK) and unaffected (PFTS-HLTHY) pigs from an affected farm, and unaffected pigs from 2 unaffected farms, were examined. The most prevalent lesions in PFTS-SICK pigs were superficial lymphocytic fundic gastritis, atrophic enteritis, superficial colitis, lymphocytic and neutrophilic rhinitis, mild nonsuppurative meningoencephalitis, and thymic atrophy. Rotavirus A and Betacoronavirus 1 (Porcine hemagglutinating encephalomyelitis virus) were identified only in PFTS-SICK pigs, but the significance of the viruses is uncertain because PFTS is not consistent with the typical presentation following infection by these pathogens. Porcine reproductive and respiratory syndrome virus, Porcine circovirus-2, Influenza A virus, Alphacoronavirus 1 (Transmissible gastroenteritis virus), Torque teno virus 1, Brachyspira hyodysenteriae, and Brachyspira pilosicoli were not identified in PFTS-SICK pigs. Suid herpesvirus 2 (Porcine cytomegalovirus), Porcine enteric calicivirus, Torque teno virus 2, pathogenic Escherichia coli, and coccidia were detected in both PFTS-SICK and PFTS-HLTHY pigs. It was concluded that there is a lack of compelling evidence that PFTS is caused by any of these pathogens.

Udvikling af pille-doserings teknologi

DEFF Research Database (Denmark)

Brændgaard, Rikke; Churchill, Inge-Lise; Bolvig Poulsen, Søren

2015-01-01

Lab. X har i samarbejde med Brønderslev Kommune og Firmaet LT Automation i et idegenereringsforløb omhandlende viden om "pillens vej", indhentet viden om udfordringer og barrierer i forbindelse med udlevering af medicin fra apoteket til den afslutningsvis indtages af borgeren. Formålet med unders...

Delevalueringer af teknologi og velfærd i 4skit

DEFF Research Database (Denmark)

Miller, Tanja; Haslam, Trine Lolk; Abildgaard, Katrine Copmann

Som et led i evaluering af satspuljeprojekt i regi af AMU - Aalborg har CEPRA udført en række delevaluering af udvalgte projekter......Som et led i evaluering af satspuljeprojekt i regi af AMU - Aalborg har CEPRA udført en række delevaluering af udvalgte projekter...

Frisættelse af fødderne

DEFF Research Database (Denmark)

Kirkeby, Ole Fogh

2013-01-01

Kropskultur. Amerikansk journalist og forfatter hævder, at løb - og fødder - giver adgang til livets mening. Anmeldelse af: Christoffer McDougall: Født til løb. Oversat af Jacob Giese efter Born to Run. Forlaget Turbulenz, 400 sider.......Kropskultur. Amerikansk journalist og forfatter hævder, at løb - og fødder - giver adgang til livets mening. Anmeldelse af: Christoffer McDougall: Født til løb. Oversat af Jacob Giese efter Born to Run. Forlaget Turbulenz, 400 sider....

Distribution af video – lige meget hvordan?

Directory of Open Access Journals (Sweden)

Diba Markus

2009-04-01

Full Text Available Artiklen omhandler løsninger til distribution af video og de faktorer, der kan være udslagsgivende, når man som underviser eller universitet skal vælge, hvilken man vil gøre brug af. De kommercielle løsninger såsom YouTube m.fl. er kendt af de fleste. Hvad færre måske ved er, at der eksisterer alternativer. På europæisk plan har en række institutioner og forskningsnet, herunder det danske, således valgt at lave deres egne løsninger. Der er afgørende forskelle i disse hhv. kommercielle og ikke-kommercielle videodistributionstjenesters tilgange til problemstillinger vedrørende adgangsbegrænsning, ophavsret, metadatering og bevaring samt kvalitetssikring af indhold - forskelle, der for en stor del udspringer af deres respektive opdrag og muligheder for brugerautentificering. Kun de færreste af alternativerne kan bruges af danske studerende og universitetsansatte. Som der argumenteres for i artiklen, er disse problemstillinger imidlertid vigtig baggrundsinformation, når der skal tages stilling til, hvordan man bedst distribuerer videoressourcer. I artiklen beskrives overordnede fællestræk ved hhv. de kommercielle og de ikke-kommercielle distributionsløsninger, og de helt grundliggende forskelle mellem dem diskuteres.

Benchmarking af udsættelser i kommunerne

DEFF Research Database (Denmark)

Christensen, Gunvor; Jeppesen, Anders Gade; Markwardt, Kristoffer

2015-01-01

nuværende tidspunkt stadig mangler relevante forhold i benchmarking-modellen. Eksempelvis antallet af stofmisbrugere, antallet af borgere med psykiatriske indlæggelser samt forekomsten af projekter med henblik på at forebygge udsættelser (fx gældsrådgivning). Disse data er først tilgængelige i 2015....

Brugen af personas hos danske virksomheder - 2012/13

DEFF Research Database (Denmark)

Nielsen, Lene; Nielsen, Kira Storgaard

2013-01-01

Denne rapport er skrevet på baggrund af et projekt initieret og finansieret af InfinIT – Innovationsnetværket for IT. Formålet med projektet er at undersøge hvorledes personas i dag etableres, kommunikeres, anvendes og vedligeholdes i forskellige typer af virksomheder samt etablere et opdateret sæt...... af anbefalinger for deres anvendelse. Virksomheders erfaringer med personas vil blive adresseret fra to forskellige vinkler:. Fokus vil bl.a. være på hvordan personas udvikles og vedligeholdes, om og hvordan de bruges, og hvad de bruges til. Personas er beskrivelser af fiktive brugere og bruges i...... designprocesser. Beskrivelserne er skabt på baggrund af data indsamlet om slutbrugerne. En samling af personas, som regel mellem 2 og 6 beskrivelser, vil i denne rapport benævnes et personasæt. Når personabeskrivelserne indgår i designprocessen bruges de til at få ideer til og beskrive, hvordan brugerne vil...

Svingningsundersøgelse af betonskorsten

DEFF Research Database (Denmark)

Dalgaard, Lars-Bo; Kristensen, Peter

Dette projekt omhandler en teoretisk og en eksperimentel undersøgelse af svingningsforholdene for en 110 meter høj betonskorsten. Skorstenen tilhører I/S Nordjyllands elektricitetsforsyning(NEFO) og er opført i forbindelse med en udbygning af Vendsysselværket med en sektion II med en turbine effe...... på 300 MW. Vendsysselværkets sektion I og II er afbilledet i figur 1.1.1, hvor værket er set fra nordøst med den undersøgte skorsten til højre i billedet. Skorstenen er projekteret af firmaet Rambøll og Hannemann og taget i brug i foråret 1977....

Værdisætning af natur- og kulturgoder. Et metodestudie af betydningen for ændringer i skala og betalingsformat

DEFF Research Database (Denmark)

Hasler, Berit; Jacobsen, Jette Bredahl; Lundhede, Thomas

aggregering og skalaproblemer. Denne rapport beskriver og tester tre af disse problemstillinger. Testene er udført som delundersøgelser ("splitsamples"), hvor det samme spørgeskemamateriale anvendes og ændres separat for hvert split. En undersøgelse af betalingsviljen for restaurering og gennemførelse af et...

Masser af information uden betydning

DEFF Research Database (Denmark)

Brier, Søren

en diskussion af informationsteorien i Tor Nørretranders "Mærk verden" og en skitse til et alternativ baseret på anden ordens kybernetik og semiotik......en diskussion af informationsteorien i Tor Nørretranders "Mærk verden" og en skitse til et alternativ baseret på anden ordens kybernetik og semiotik...

Effekten af absolut kumulation

DEFF Research Database (Denmark)

Kyvsgaard, Britta; Klement, Christian

2012-01-01

Som led i finansloven for 2011 blev regeringen og forligspartierne enige om at undersøge reglerne om strafudmåling ved samtidig pådømmelse af flere kriminelle forhold og i forbindelse hermed vurdere konsekvenserne af at ændre de gældende regler i forhold til kapacitetsbehovet i Kriminalforsorgens...... samlet bødesum ved en absolut kumulation i forhold til en modereret kumulation, som nu er gældende....

Portræt af litteraturkritikeren som ungt menneske

DEFF Research Database (Denmark)

Schatz-Jakobsen, Claus

2004-01-01

Artiklen præsenterer den amerikanske litteraturkritiker Geoffrey Hartmans tidlige forfatterskab og fremdrager dets selvbiografiske undertekst som nødvendig effekt af den unge Hartmans fascination af den engelske romantiker William Wordsworths forfatterskab.......Artiklen præsenterer den amerikanske litteraturkritiker Geoffrey Hartmans tidlige forfatterskab og fremdrager dets selvbiografiske undertekst som nødvendig effekt af den unge Hartmans fascination af den engelske romantiker William Wordsworths forfatterskab....

Uddrag af Ph.d.-afhandlingen Den groteske modernisme

DEFF Research Database (Denmark)

Lübker, Henrik

2011-01-01

Om forholdet mellem "det groteske" og "den groteske modernisme" eksemplificeret i en læsning af E.A. Poes "The Fall of the House of Usher" og flere af Francis Bacons malerier.......Om forholdet mellem "det groteske" og "den groteske modernisme" eksemplificeret i en læsning af E.A. Poes "The Fall of the House of Usher" og flere af Francis Bacons malerier....

Virkelighedsflugt og masser af sex

DEFF Research Database (Denmark)

Lorentzen, Rasmus Fink

2011-01-01

I artiklen laves en læsning af ungdomsromanen "6" (2010) af Ronni Andersen. Dernæst udfoldes en række litteraturpædagogiske forslag tilen undervisning i romanen i skolens ældste klasser. Arbejdsforslagene er baseret på en kompetencetilgang til danskfaget og rummer fire værksteder hvor eleverne sk...

Forført af hjernen?

DEFF Research Database (Denmark)

Lieberoth, Andreas

2013-01-01

Blandingen af offentlig interesse, gode mediehistorier og smarte sælgere har ført til sejlivede neuromyter, der ikke bare distraherer almindelige mennesker, men også fører til udbredte misforståelser blandt professionelle som lærere og plejepersonale. I bedste fald leder neuromyter til spild af t...

Characterization of porcine MMP-2 and its association with immune traits

DEFF Research Database (Denmark)

Huang, Honggang; Zhao, Weimin; Tang, Zhonglin

2009-01-01

cloned the 5'-upstream sequence, 3'-downstream sequence as well as other missed genomic sequences of porcine MMP-2, the genomic structure and the promotor sequence were analyzed and found to share high similarity with those of human MMP-2. Porcine MMP-2 was assigned to SSC6p14-p15, and closely linked......Matrix metalloproteinase-2 (MMP-2) plays important roles in inflammation and immunity besides its basic role in degrading and remodelling extracellular matrix (ECM). The expression of MMP-2 is up-regulated in many human as well as animal models of inflammatory and immune diseases. In this study, we...... to microsatellite SW1108 (53cR, LOD score 7.59) by IMpRH panel. Real-time PCR analysis revealed that the expression of porcine MMP-2 was remarkably different in diverse tissues, a high level expression was observed in the testis and uterus, relatively low expression in other tissues. Allele frequencies...

Æstetiseringen af forbruget: En undersøgelse af to udstillinger, hvor branding er kunst - og kunst er branding

DEFF Research Database (Denmark)

Hermansen, Judy

2008-01-01

Æstetiseringen af hverdagen er ét af de helt centrale træk ved det postmoderne forbrugersamfund. Og denne nedbrydning af grænserne mellem kunst og forbrug er det også vigtigt for marketingteorien at udforske. Artiklen analyserer to (kunst?)udstillinger i England, som på hver sin måde tematiserer,...

Forhastet regulering af de store

DEFF Research Database (Denmark)

Thomsen, Steen

2013-01-01

Christiansborg gennemfører sandsynligvis en markant skærpet regulering af de store finansielle virksomheder. Det vil virke kontraktivt og medvirke til erhvervslivets kredittørke.......Christiansborg gennemfører sandsynligvis en markant skærpet regulering af de store finansielle virksomheder. Det vil virke kontraktivt og medvirke til erhvervslivets kredittørke....

Ledelse af forandringer i folkeskolen

DEFF Research Database (Denmark)

Kjer, Mikkel; Rosdahl, Anders

2016-01-01

Dette notat bygger på kvalitative interview på seks folkeskoler i efteråret 2015. Skolerne ligger i forskellige egne af landet og i landområder, mindre og store byer. Skolerne er af forskellig størrelse, og deres elevgrundlag, dvs. forældrenes sociale baggrund, varierer også. Notatet bygger primæ...

Online lektiehjælp – Udvikling af en vejledningsdidaktik med pædagogisk designforskning som metode

Directory of Open Access Journals (Sweden)

Jens Jørgen Hansen

2016-05-01

Full Text Available Lektievejledning er en pædagogisk praksis i rivende udvikling med fokus på at støtte elever i at forstå og håndtere udfordringer i deres lektiearbejde. Lektievejledning er på den politiske dagsorden i skole og ungdomsuddannelser og tilbydes både internt af uddannelsesinstitutionerne samt af private og offentlige aktører. Men lektievejledning er ikke etableret som et teoretisk felt eller som en særlig vejledningskompetence. Der er således behov for at begrebsliggøre og udvikle teori om, hvad lektievejledning er, og hvordan vejledningsformen didaktisk kan håndteres. Mere konkret er ud-fordringen at udvikle et nuanceret fagsprog samt en modellering af feltet til forklaring af lektievejledningens komplekse praksis med det mål at udvikle og kvalificere lektievejledning som pædagogisk teori og pædagogisk prak-sis. Denne artikel præsenterer resultatet af et forskningsprojekt, hvis mål er at udvikle en vejledningsdidaktik, herunder metoder, modeller og materialer for lektievejledning i regi af Lektier Online. Lektier Online er en organisation ved Statsbiblioteket i Århus, der tilbyder en online lektiecafe, hvor bl.a. gymnasie-elever kan få hjælp af frivillige universitetsstuderende. Dette didaktiske de-sign er et eksempel på, hvordan digitalisering og ny teknologi er en katalysator for udvikling af nye innovative læringsmiljøer og læringstilbud, der åbner fleksible og stedsuafhængige rum for læring, giver mulighed for samspil med aktører uden for skolens formelle rammer, og som er båret af integration af digitale teknologier i læringssituationen. Artiklen beskriver forskningsproces-sen med at indkredse en vejledningsdidaktik for lektiehjælp samt udviklingen, konceptualiseringen, afprøvningen og evalueringen af en række vejlednings-modeller på grundlag af forskningsmetoden Pædagogisk designforskning og dens tradition for brugerdreven innovation. Artiklen beskriver for det første resultatet af forskningsprocessen i form

Miljøoptimering af afvaskning ved tryk med vandfortyndbar flexotrykfarve

DEFF Research Database (Denmark)

Larsen, Henrik Fred; Helweg, Christian; Pedersen, Anne Rathmann

Rapporten beskriver praksis ved afvaskning af vandfortyndbare flexotrykfarver i emballageindustrien og anviser muligheder for indførelse af renere teknologi til reduktion af spildevandsbelastningen. Anbefalingerne omfatter a) udformning af farveværker med henblik på effektiv tømning af farve og e...

Tachykinins in the porcine pancreas

DEFF Research Database (Denmark)

Schmidt, P T; Tornøe, K; Poulsen, Steen Seier

2000-01-01

The localization, release, and effects of substance P and neurokinin A were studied in the porcine pancreas and the localization of substance P immunoreactive nerve fibers was examined by immunohistochemistry. The effects of electrical vagus stimulation and capsaicin infusion on tachykinin release...... and the effects of substance P and neurokinin A infusion on insulin, glucagon, somatostatin, and exocrine secretion were studied using the isolated perfused porcine pancreas with intact vagal innervation. NK-1 and NK-2 receptor antagonists were used to investigate receptor involvement. Substance P immunoreactive...

Udkantsdanmark: Avisernes (med)produktion af Nordjyllands territorielle stigma

DEFF Research Database (Denmark)

Pristed Nielsen, Helene; Christensen, Sascha

2013-01-01

Artiklen undersøger hvordan Region Nordjylland (NJ) beskrives i medierne og hvordan diskursen om Udkanten dominerer avisernes fremstilling af regionen . Baggrunden herfor er en interesse for social ulighed, og i denne sammenhæng Wacquants begreb om territorielt stigma (1996, 2007). Wacquant føjer...... stigma. I Gunnar Lind Haase Svendsens studie af italesættelsen af ’udkanten’ fra 1996-2011 ses fx en gennem de seneste år stigende anvendelse af termer som ’den rådne banan’ og ’Udkantsdanmark’ i medierne (Svendsen 2013: 12). Dette stigma forbindes med flere dele af Danmark (se bl.a. Arbejderbevægelsens...

Vidensledelse og den sociale dimension i deling af viden

DEFF Research Database (Denmark)

Lauring, Jakob

2004-01-01

imidlertid et alternativt syn på viden som i højere grad inddrager den sociale dimension ved vidensdeling - den socialkonstruktivistiske opfattelse. I denne artikel vil jeg beskæftige mig med den socialkonstruktivistiske opfattelse af viden og beskrive, hvorledes denne synsvinkel kan rette fokus mod nogle...... meget væsentlige problemstillinger ved organisatorisk adfærd, som man ofte kommer til at ignorere, hvis man i for høj grad baserer ledelsen på en funktionalistisk opfattelse af viden. Delingen af viden er en aktuel udfordring for anvendelse af viden i forretningssammenhæng. Det er hovedsageligt det, som...... gør vidensbegrebet interessant i forhold til ledelse af virksomheder. - Fokus på de sociale barrierer for deling af viden Jeg vil i det kommende illustrere, hvilke betydninger den sociale dimension i deling af viden kan have for videnledelse. Det vil jeg gøre gennem beskrivelser af to forskellige...

Second-generation magnesium scaffold Magmaris: device design and preclinical evaluation in a porcine coronary artery model.

Science.gov (United States)

Waksman, Ron; Zumstein, Philine; Pritsch, Martin; Wittchow, Eric; Haude, Michael; Lapointe-Corriveau, Capucine; Leclerc, Guy; Joner, Michael

2017-07-20

The second-generation drug-eluting absorbable magnesium scaffold Magmaris, recently introduced for the treatment of obstructive coronary atherosclerotic lesions, suggests a good safety profile, but preclinical assessment is important for predicting clinical performance. The aim of the present study was to assess subacute and long-term safety as well as pharmacokinetic properties of the Magmaris compared with a current-generation metallic DES and an approved BRS in porcine and rabbit animal models. Ninety Magmaris scaffolds were implanted into non-diseased porcine and rabbit models. A bioresorbable vascular scaffold (Absorb) and a permanent drug-eluting stent (XIENCE Xpedition) served as controls. Scanning electron microscopy showed increased endothelialisation and decreased thrombus formation at three and 28 days in the Magmaris group compared with the Absorb group. In the XIENCE group, inflammation exceeded the level in the Magmaris group at 365 and 730 days. Neointimal growth was greater in the Magmaris group than in the XIENCE group. Late lumen loss decreased over time in both groups. Optical coherence tomography (OCT) showed stable luminal dimensions in both the Magmaris and XIENCE groups. Pharmacokinetic studies demonstrated a retarded elution profile in the Magmaris group with 69.4% of sirolimus released at 90 days. Preclinical results suggest that the Magmaris has a favourable safety profile with advanced healing relative to benchmark, low acute thrombogenicity, and absence of excessive lumen loss up to two years. These results support clinical application of Magmaris for human use.

Reliable porcine coronary model of chronic total occlusion using copper wire stents and bioabsorbable levo-polylactic acid polymer.

Science.gov (United States)

Sim, Doo Sun; Jeong, Myung Ho; Cha, Kyoung Rae; Park, Suk Ho; Park, Jong Oh; Shin, Young Min; Shin, Heungsoo; Hong, Young Joon; Ahn, Youngkeun; Schwartz, Robert S; Kang, Jung Chaee

2012-12-01

Chronic total occlusion (CTO) remains a challenge in interventional cardiology. We investigated the feasibility and reliability of copper wire stents and levo-polylactic acid (l-PLA) as a means of CTO induction in a porcine model. In one group of 20 swine, copper stents were crimped on a 3.0mm angioplasty balloon and inserted into the mid-left anterior descending coronary artery (LAD). In the other group of 20 swine, l-PLA was wrapped on a guidewire and pushed into the distal LAD with a 3.0mm balloon catheter to induce embolization. Of 20 swine which underwent copper stent implantation, 13 died of stent thrombosis. In the remaining 7 swine, total or near total occlusion with collateral circulation was observed at 5 weeks. Of 20 swine which underwent l-PLA embolization, 4 died of ventricular fibrillation during or shortly after the procedure. Serial histopathologic studies showed complete absorption of the polymer with replacement by fibrotic tissue approximately 4 weeks following the polymer implantation. CTO could be reliably induced in porcine coronary arteries by copper stents and l-PLA. These models may support investigation of new percutaneous devices to facilitate CTO interventions. Copyright © 2012 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.

Analyse og design af produktionssystemer

DEFF Research Database (Denmark)

Johansen, John; Riis, Jens Ove; Arlbjørn, Jan Stentoft

lys, men til vores store glæde og overraskelse har vi erfaret, at dele af materialet stadigvæk er anvendt på en del ingeniør- og økonomiuddannelser i Danmark. Nogle af de tanker og metoder som blev udviklet i ViPSprogrammet har overlevet og opfattes fortsat som relevante og anvendelige også til...

Effektivitet af naturligt drevne radontiltag året rundt

DEFF Research Database (Denmark)

Haker Høegh, Britt; Rasmussen, Torben Valdbjørn

Denne rapport omhandler projektet Effektivitet af naturligt drevne radontiltag året rundt. Projektet er et spireprojekt finansieret af Innovationsnetværket InnoBYG og Forsknings- og Innovationsstyrelsen. Projektet er gennemført af Teknologisk Institut i samarbejde med Statens Byggeforskningsinsti...

Produktion og afsætning af nicheprodukter

DEFF Research Database (Denmark)

Jensen, Jonas Baungaard; Hansen, Henning Otte

2016-01-01

. Egnsmærker opnår generelt en merpris på 20-30 pct. Det er dog bemærkelsesværdigt, at landmanden “kun” har omkring 13 pct. af forbrugerkronen på Lammefjords-kartofler. Her er der tale om unikke produkter, hvor det i praksis udelukkende er landmanden og landmandens placering og produktion, som skaber de unikke...

Evaluering af den ernæringsmæssige kvalitet af madordninger i daginstitutioner

DEFF Research Database (Denmark)

Nielsen, Marie Heyn; Lassen, Anne Dahl; Christensen, Lene Møller

Fokus på madordninger i daginstitutioner og kvaliteten heraf har været stigende de seneste år. Mange nye madordninger er etableret i løbet af 2009 og 2010 især i børnehaver og i børnehavegrupper i de integrerede daginstitutioner. I 2009 opstillede Fødevarestyrelsen anbefalinger for den ernæringsm...... fedt samt at servere fisk, grøntsager, kartofler, samt fuldkornsvarianter af ris, pasta og brød mere hyppigt....

A model study on color and related structural properties of cured porcine batters

NARCIS (Netherlands)

Palombo, R.

1990-01-01

Color, determined by tristimulus colorimeters, and related structural properties, i.e., microstructure, surface rheology, and bulk rheology, of cured porcine meat batters were studied.

Effects of various processing factors (such as, temperature, air pressure during chopping, and

Sammenligning af kvaliteten af kommunernes sagsbehandling

DEFF Research Database (Denmark)

Rasmussen, Martin

flere forskellige spørgsmål fra praksis¬undersøgelser i samme sammenligning. Vi foreslår også, at metoder til såkaldte ’multi¬ple sammenligninger’ bruges til dele af undersøgelserne. Det er en ældre og ikke særlig kendt metode, men den er særdeles relevant både i den konkrete anvendelse og i andre...

Brief report: biomarkers of aortic vascular prosthetic graft infection in a porcine model with Staphylococcus aureus

DEFF Research Database (Denmark)

Langerhuus, S. N.; Tønnesen, E. K.; Jensen, K. H.

2010-01-01

Aortic vascular prosthetic graft infection (AVPGI) with Staphylococcus aureus is a feared post-operative complication. This study was conducted to evaluate the clinical signs and potential biomarkers of infection in a porcine AVPGI model. The biomarkers evaluated were: C-reactive protein (CRP......), fibrinogen, white blood cells (WBC), major histocompatibility complex II (MHC II) density, lymphocyte CD4:CD8 ratio and tumour necrosis factor-alpha (TNF-α) in vitro responsiveness. Sixteen pigs were included in the study, and randomly assigned into four groups (n = 4): “SHAM” pigs had their infra...

Undersøgelse af mulighederne for anvendelse af open source Integrated Library Systems (ILS) i universitetsbiblioteket

DEFF Research Database (Denmark)

Greve, Eli; Bøgh Pedersen, Kasper

Dette projekt har til formål, at to biblioteker med meget forskellige profiler gennemfører test af konkrete open source baserede ILS systemer, formidler resultaterne til FFU-bibliotekerne og deres interessenter, og herved bidrager til en nuancering af beslutningsprocesserne i de kommende år omkring...

Anmeldelse af Eigil Lego Andersen: Dirigent og generalforsamling

DEFF Research Database (Denmark)

Werlauff, Erik

2011-01-01

Artiklen er en anmeldelse af advokat, adj. professor, dr. jur. Eigil Lego Andersens juridiske monografi om dirigenten og generalforsamlingen, herunder med lancering af en række nye afstemningsmetoder til brug for generalforsamlingen i (større) aktieselskaber.......Artiklen er en anmeldelse af advokat, adj. professor, dr. jur. Eigil Lego Andersens juridiske monografi om dirigenten og generalforsamlingen, herunder med lancering af en række nye afstemningsmetoder til brug for generalforsamlingen i (større) aktieselskaber....

Forsegling af okklusal dentincaries i permanente molarer

DEFF Research Database (Denmark)

Qvist, Vibeke; Borum, Mette Kit; Dynes Møller, Kirsten

2017-01-01

Der findes ingen eksakte tal for forekomsten af fødevareallergi i den danske befolkning. Den kumulerede forekomst af fødevareallergi hos børn i de første tre leveår skønnes at være på 7-8 %, hvoraf hovedparten vokser sig fra det. Punktprævalensen af diagnosticeret fødevareallergi hos voksne er 3 ...

Succesfulde virksomheder ledes af mænd - Ikke succesfulde af kvinder?

DEFF Research Database (Denmark)

Madsen, Mona Toft; Madsen, Henning; Neergaard, Helle

fra amerikanske og britiske undersøgelser (Schein, 1973, 75, 96; Ryan et al., 2005a) ved at teste dem i et kulturelt miljø karakteriseret af mere feminine ledertræk (Hofstede, 1991). Undersøgelsen tager udgangspunkt i et sæt af person-karakteristika, der er blevet anvendt til at undersøge sammenfald...... mellem mandlige karakteristika og succesfuld ledelse (Schein, 1973, 75, 96). På lige fod med nyere britiske undersøgelser (Ryan et al. 2005a), er den aktuelle undersøgelse imidlertid udvidet til også at omhandle lederes perceptioner i forhold til ledelse i virksomheder uden succes. Resultaterne viser...

Læremiddeltjek af Fandange - dansk for 5. klasse af Trine May og Susanne Arne-Hansen

DEFF Research Database (Denmark)

Christensen, Mette Vedsgaard

2013-01-01

Fandango 5 er skrevet af Trine May og Susanne Arne-Hansen og er første gang udgivet i 2009. Systemet er siden kommet i flere oplag. Fandango – dansk for 5. klasse består af en grundbog (264 sider) Arbejdsbog A (64 sider) Arbejdsbog B (64 sider), en lærervejledning (ca. 220 sider) og en hjemmeside...

Udvikling af en internetsøgestrategi til identifikation af ny medicinsk teknologi inden for prostatacancer

DEFF Research Database (Denmark)

Thygesen, Marianne; Vondeling, Hindrik; Douw, Karla

2007-01-01

internetsider efter efficiens. Her er efficiens forstået som mindst antal minutter brugt pr. fund af information om en ny væsentlig medicinsk teknologi. Resultater: Strategien består af ti sider, som bør benyttes i følgende rækkefølge: YahooHealth, DoctorsGuide, The National Electronic Library for Medicines...

Metodik til studiet af strategiske ledelsesprocesser i organisationer

Directory of Open Access Journals (Sweden)

Jørgen Gulddahl Rasmussen

2010-10-01

Full Text Available Denne artikel handler om at skabe viden om en hverdagspraksis, der sjældent er sat ord på. Den behandler de ledelsesprocesser, der i virksomheder producerer strategi og omsætter sådanne strategier i produktive handlinger. Det er processer, der i små og mellemstore virksomheder er præget af intuition og af meget uformelle samspil mellem virksomhedsledere og medarbejdere. Metodikken er en videreudvikling af de metoder til produktion af kvalitative data, som FIRM-gruppen har eksperimenteret med gennem nogle år. Artiklen kan ses som en afrapportering af et flerårigt metodeudviklingsarbejde. Da erfaringer fra sådanne virksomheder viser, at strategiske processer i høj grad udvikler sig gennem uformel kommunikation, og at lederes intuition spiller en meget stor rolle, arbejder metodikken med en struktureret udviklingsdialog mellem et mindre antal virksomhedsledere med strategiske opgaver og forskere. Denne udviklingsdialog har til formål at følge og forstå de processer, der skaber ledernes egne praksisrelaterede ”teorier” for strategisk handling. Efter en præsentation af temaet gennemgås metodikkens praktiske fremgangsmåde med undersøgelser i en række virksomheder, opbygning af cases i samarbejde med ledere fra disse virksomheder, diskussion af disse og foreløbige konklusioner hen mod de næste trin i metodikken. Artiklen er skrevet samtidigt med arbejdet på et igangværende forskningsprojekt og vil derfor både være en præsentation og en udvikling af muligheder og vanskeligheder i metodikken.

Skattemæsssig kvalifikation af trusts i praksis

DEFF Research Database (Denmark)

Feldthusen, Rasmus Kristian

2013-01-01

Artiklen har til formål at systematisere og redegøre for skattemyndighedernes fremgangsmåde og kvalifikation af trusts i dansk ret. Baggrunden herfor er, at antallet af trust-sager for såvel de administrative myndigheder som domstolene relativt set er vokset betydeligt siden årtusindeskiftet. Ud...... fra en gennemgang af doms- og administrativ praksis kan det konstateres, at kvalifikationen af trusts kan opdeles i fire hovedkategorier, som er væsentlige at have kendskab til, såfremt en skattesag indeholdende trust-elementer måtte være under opsejling. Endvidere inddrages skattestrafferetlig...... praksis på baggrund af en nyligt afsagt landsretsdom vedrørende en offshore trust....

Porcine circovirus diseases

Directory of Open Access Journals (Sweden)

Ristoski Trpe

2009-05-01

Full Text Available Porcine circovirus type 2 belongs on the family Circoviridae. This virus family includes small, non-enveloped viruses, with a circular, single-standed DNA genome.This virus causes mainly subclinical infections, but a number of diseases have been linked to it (porcine circovirus diseases, PCVD. The most economically important PCVD is postweaning multisystemic wasting syndrome (PMWS, which mainly affects pigs of 2 to 5 months of age, with progressive wasting, diarrhea and respiratory disorders. Main PMWS lesions are found in lymphoid tissues, which are characterized by lymphocyte depletion with granulomatous (histiocytic and multinucleate giant cell infiltration. PMWS is considered as multifactorial disease, with a number of infectious and non-infectious factors able to act as disease triggering in PCV2 infected pigs. PCVDs are worldwide distributed, and PMWS was diagnosed in Macedonia in 2007.

Rating af banker og banksystemer

DEFF Research Database (Denmark)

Grosen, Anders

2010-01-01

Banksystemer over hele verdenen er med nød og næppe blevet reddet af diverse statslige hjælpepakker. Derfor er der i disse år øget fokus på banker og banksystemers rating. Denne interesse bliver ikke mindre af, at de statslige garantier til bankerne i mange lande udløber i de kommende år. Rating ...

Comparison of Acute Thrombogenicity for Magnesium versus Stainless Steel Stents in a Porcine Arteriovenous Shunt Model.

Science.gov (United States)

Lipinski, Michael J; Acampado, Eduardo; Cheng, Qi; Adams, Lila; Torii, Sho; Gai, Jiaxiang; Torguson, Rebecca; Hellinga, David G; Joner, Michael; Harder, Claus; Zumstein, Philine; Finn, Aloke V; Kolodgie, Frank D; Virmani, Renu; Waksman, Ron

2018-05-08

Aims The aetiology for reduced thrombogenicity of the Magmaris resorbable magnesium scaffold (RMS) when compared with the Absorb bioresorbable vascular scaffold remains unclear. We therefore investigated whether the Magmaris RMS has platelet-repelling properties by comparing its acute thrombogenicity with an equivalent stainless steel stent in an arteriovenous shunt model. Methods and Results An ex vivo porcine carotid jugular arteriovenous shunt was established and connected to Sylgard tubing containing the Magmaris RMS with sirolimus-eluting PLLA coating and an equivalent 316L stainless steel stent with sirolimus-eluting PLLA coating. Six shunts (2 shunt runs per pig) were run comparing the 2 scaffolds (n=9) in alternating order. Nested generalised linear mixed models were employed to compare variables between scaffold groups. Confocal fluorescent microscopy costaining CD61/CD42b demonstrated that the 316L equivalent stent had significantly greater platelet coverage of the total scaffold compared with Magmaris (5.8% vs. 2.8%, Rate ratio 2.21 [1.41, 3.47], p=0.012). Scanning electron microscopy demonstrated significantly greater thrombus deposition on the 316L equivalent stent as a percentage of the total scaffold compared with Magmaris (8.0% vs. 5.3%, p=0.009). Magmaris also had significantly less CD14 positive monocyte deposition and a trend toward less PM-1 positive neutrophil compared with the 316L equivalent stent. Conclusion Despite having identical scaffold characteristics regarding geometrical design, Magmaris had significantly less thrombogenicity and inflammatory cell deposition compared with the equivalent 316L stainless steel stent in a porcine arteriovenous shunt model. These data suggest resorbable magnesium scaffolds may have inherent properties that reduce adhesion of platelets and inflammatory cells.

The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms

KAUST Repository

Park, Hyo-Young

2017-04-21

The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms. These proteins also move rapidly and continuously in the nuclei, and their movements are affected by ATP depletion. The U2AF65 proteins are splicing factors that interact with SF1 and U2AF35 proteins to promote U2snRNP for the recognition of the pre-mRNA 3\\' splice site during early spliceosome assembly. We have determined the subcellular localization and movement of these proteins\\' Arabidopsis homologs. It was found that Arabidopsis U2AF65 homologs, AtU2AF65a, and AtU2AF65b proteins interact with AtU2AF35a and AtU2AF35b, which are Arabidopsis U2AF35 homologs. We have examined the mobility of these proteins including AtSF1 using fluorescence recovery after photobleaching and fluorescence loss in photobleaching analyses. These proteins displayed dynamic movements in nuclei and their movements were affected by ATP depletion. We have also demonstrated that these proteins shuttle between nuclei and cytoplasms, suggesting that they may also function in cytoplasm. These results indicate that such splicing factors show very similar characteristics to their human counterparts, suggesting evolutionary conservation.

The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms

KAUST Repository

Park, Hyo-Young; Lee, Keh Chien; Jang, Yun Hee; Kim, SoonKap; Thu, May Phyo; Lee, Jeong Hwan; Kim, Jeong-Kook

2017-01-01

The Arabidopsis splicing factors, AtU2AF65, AtU2AF35, and AtSF1 shuttle between nuclei and cytoplasms. These proteins also move rapidly and continuously in the nuclei, and their movements are affected by ATP depletion. The U2AF65 proteins are splicing factors that interact with SF1 and U2AF35 proteins to promote U2snRNP for the recognition of the pre-mRNA 3' splice site during early spliceosome assembly. We have determined the subcellular localization and movement of these proteins' Arabidopsis homologs. It was found that Arabidopsis U2AF65 homologs, AtU2AF65a, and AtU2AF65b proteins interact with AtU2AF35a and AtU2AF35b, which are Arabidopsis U2AF35 homologs. We have examined the mobility of these proteins including AtSF1 using fluorescence recovery after photobleaching and fluorescence loss in photobleaching analyses. These proteins displayed dynamic movements in nuclei and their movements were affected by ATP depletion. We have also demonstrated that these proteins shuttle between nuclei and cytoplasms, suggesting that they may also function in cytoplasm. These results indicate that such splicing factors show very similar characteristics to their human counterparts, suggesting evolutionary conservation.

Electroejaculation functions primarily by direct activation of pelvic musculature: Perspectives from a porcine model

Directory of Open Access Journals (Sweden)

Adam M.R. Groh

2018-03-01

Full Text Available Ejaculatory dysfunction is a significant cause of infertility in men that have incurred spinal cord injury or iatrogenic lesions to the sympathetic nerves in the retroperitoneum. For such patients, electroejaculation – whereby a voltage is applied transrectally under general anesthesia – is a highly-effective procedure to obtain ejaculate. At present, however, there remains uncertainty as to the physiological mechanism by which electroejaculation prompts seminal emission in males with neurogenic anejaculation. Thus, in the present study, we aimed to determine, for the first time, whether electroejaculation functions by mimicking a neurophysiological response, or by directly activating local pelvic musculature. Using electroejaculation in a novel porcine model, we monitored the strength of contraction of the internal urethral sphincter (a smooth muscle involved in ejaculation before and after lesioning its sympathetic innervation with a combination of progressively-worsening surgical and pharmacological insults in three anesthetized boars (46.1 ± 7.4 kg. Importantly, prior to this investigation, we confirmed the comparative structural anatomy of the porcine model to humans through gross dissection and histological analysis of the infrarenal retroperitoneal sympathetic nerves and ganglia in 18 unembalmed boars. Prior to sacrifice, three of these boars underwent functional testing to confirm control of the internal urethral sphincter by the hypogastric nerves. Our results demonstrate that electroejaculation-induced contraction of the internal urethral sphincter was preserved following each progressive neural insult compared to the control state (p > 0.05. In contrast, these same insults resulted in paralysis/paresis of the internal urethral sphincter when its sympathetic innervation was directly stimulated with bipolar electrodes (p < 0.05. Taken together, our results provide the first empirical evidence to suggest that

Centralisering af FM organisationer

DEFF Research Database (Denmark)

Nielsen, Susanne Balslev

2016-01-01

Centralisering af FM organisationer kan let føre til mange frustrerende arbejds- dage for medarbejdere og deres ledere. Denne artikel giver et bud på hvad kom- muner og andre kan gøre for at komme bedre igennem en omorganisering.......Centralisering af FM organisationer kan let føre til mange frustrerende arbejds- dage for medarbejdere og deres ledere. Denne artikel giver et bud på hvad kom- muner og andre kan gøre for at komme bedre igennem en omorganisering....

Måling af lydisolation og efterklangstid i praksis

DEFF Research Database (Denmark)

Kristensen, J.

Meddelelsen omhandler undersøgelser af mulighederne for at forbedre reproducerbarheden ved måling af luftlydisolation, trinlydniveau og efterklangstid under betingelser, der svarer til måling i den indflytningsfærdige bygning. Der omtales resultater fra måling med to forskellige udformninger af h...

Afståelsesbeskatning ved flytning af DEMPE-funktioner

DEFF Research Database (Denmark)

Kjærsgaard, Louise Fjord; Bundgaard, Jakob

2017-01-01

OECD har i den senest opdaterede version af Transfer Pricing Guidelines fastsat en definition af immaterielle aktiver og har samtidig indført konceptet DEMPE-funktioner, der omfatter visse vigtige funktioner relateret til immaterielle aktiver. SKAT synes at være af den opfattelse, at DEMPE...

Reformationens tøvende omfortolkning af kætterbegrebet

DEFF Research Database (Denmark)

Holm, Bo Kristian; Thomsen, Søren Feldtfos

2012-01-01

Denne artikel undersøger påstanden om en omfortolkning af kætterbegrebet i den lutherske reformation. Der argumenteres for, at det giver god mening at tale om nytænkning af kætterbegrebet hos den unge Luther i form af en internalisering af kætteriet, hvor den enkeltes eget syndige selv udpeges som...

Release of galanin from isolated perfused porcine adrenal glands

DEFF Research Database (Denmark)

Holst, J J; Ehrhart-Bornstein, M; Messell, T

1991-01-01

We found a high concentration of galanin in extracts of porcine adrenal glands (114 pmol/g). By immunohistochemistry, galanin was localized to groups of medullary cells previously shown to produce norepinephrine. To study mechanisms for the release of galanin, we developed the following in vitro...... model: isolated perfused porcine adrenals with intact splanchnic nerve supply. When the nerves were electrically stimulated, epinephrine and norepinephrine secretion increased 276- and 291-fold, respectively, and galanin release increased up to 1,300-fold. Acetylcholine at 10(-6) M stimulated galanin...... release, and hexamethonium almost abolished the response to nerve stimulation. Galanin infusions had no effect on epinephrine and norepinephrine secretion in concentrations of 10(-8) and 10(-7) M, but increased both cortisol and aldosterone secretion (P less than 0.05). Splanchnic nerve stimulation...

The effect of intra-abdominal hypertension incorporating severe acute pancreatitis in a porcine model.

Directory of Open Access Journals (Sweden)

Lu Ke

Full Text Available INTRODUCTION: Abdominal compartment syndrome (ACS and intra abdominal hypertension(IAH are common clinical findings in patients with severe acute pancreatitis(SAP. It is thought that an increased intra abdominal pressure(IAP is associated with poor prognosis in SAP patients. But the detailed effect of IAH/ACS on different organ system is not clear. The aim of this study was to assess the effect of SAP combined with IAH on hemodynamics, systemic oxygenation, and organ damage in a 12 h lasting porcine model. MEASUREMENTS AND METHODS: Following baseline registrations, a total of 30 animals were divided into 5 groups (6 animals in each group: SAP+IAP30 group, SAP+IAP20 group, SAP group, IAP30 group(sham-operated but without SAP and sham-operated group. We used a N(2 pneumoperitoneum to induce different levels of IAH and retrograde intra-ductal infusion of sodium taurocholate to induce SAP. The investigation period was 12 h. Hemodynamic parameters (CO, HR, MAP, CVP, urine output, oxygenation parameters(e.g., S(vO(2, PO(2, PaCO(2, peak inspiratory pressure, as well as serum parameters (e.g., ALT, amylase, lactate, creatinine were recorded. Histological examination of liver, intestine, pancreas, and lung was performed. MAIN RESULTS: Cardiac output significantly decreased in the SAP+IAH animals compared with other groups. Furthermore, AST, creatinine, SUN and lactate showed similar increasing tendency paralleled with profoundly decrease in S(vO(2. The histopathological analyses also revealed higher grade injury of liver, intestine, pancreas and lung in the SAP+IAH groups. However, few differences were found between the two SAP+IAH groups with different levels of IAP. CONCLUSIONS: Our newly developed porcine SAP+IAH model demonstrated that there were remarkable effects on global hemodynamics, oxygenation and organ function in response to sustained IAH of 12 h combined with SAP. Moreover, our model should be helpful to study the mechanisms of IAH

Betydningen af pesticider for forekomsten af vilde bier

DEFF Research Database (Denmark)

Bruus, Marianne; Dupont, Yoko Luise; Grant, Ruth

Både i Danmark og andre steder er der observeret en nedgang i de insekter, der bestøver landmandens afgrøder og de vilde planter. Tilbagegangen falder sammen med, at landbrugsdriften er blevet mere intensiv, og anvendelsen af pesticider er øget. Vi ved imidlertid ikke, hvilken rolle pesticiderne ...

Dermal absorption behavior of fluorescent molecules in nanoparticles on human and porcine skin models.

Science.gov (United States)

Debotton, Nir; Badihi, Amit; Robinpour, Mano; Enk, Claes D; Benita, Simon

2017-05-30

The percutaneous passage of poorly skin absorbed molecules can be improved using nanocarriers, particularly biodegradable polymeric nanospheres (NSs) or nanocapsules (NCs). However, penetration of the encapsulated molecules may be affected by other factors than the nanocarrier properties. To gain insight information on the skin absorption of two fluorescent cargos, DiIC 18 (5) and coumarin-6 were incorporated in NSs or NCs and topically applied on various human and porcine skin samples. 3D imaging techniques suggest that NSs and NCs enhanced deep dermal penetration of both probes similarly, when applied on excised human skin irrespective of the nature of the cargo. However, when ex vivo pig skin was utilized, the cutaneous absorption of DiIC 18 (5) was more pronounced by means of PLGA NCs than NSs. In contrast, PLGA NSs noticeably improved the porcine skin penetration of coumarin-6, as compared to the NCs. Furthermore, the porcine skin results were reproducible when triplicated whereas from various human skin samples, as expected, the results were not sufficiently reproducible and large deviations were observed. The overall findings from this comprehensive comparison emphasize the potential of PLGA NCs or NSs to promote cutaneous bioavailability of encapsulated drugs, exhibiting different physicochemical properties but depending on the nature of the skin. Copyright © 2017 Elsevier B.V. All rights reserved.

Barriere for Studerendes Læring af Holistisk Systemudvikling

DEFF Research Database (Denmark)

Levinsen, Karin; Madsen, Sabine

Ud fra aktionsforskning af faget Informationsbehandling på bachelorprogrammet HA/DØK ved Copenhagen Business School undersøger vi i denne artikel hvilke barriere de studerende oplever i forbindelse med læring af holistisk systemudvikling og hvorfor. Siden 1980'erne har man i (dele af) litteraturen...

Performance analysis of AF cooperative systems with HPA nonlinearity in semi-blind relays

KAUST Repository

Qi, Jian

2012-12-01

In this paper, dual-hop amplify-and-forward (AF) cooperative systems in the presence of high-power amplifier (HPA) nonlinearity at semi-blind relays, are investigated. Based on the modified AF cooperative system model taking into account the HPA nonlinearity, the expression for the output signal-to-noise ratio (SNR) at the destination node is derived, where the interference due to both the AF relaying mechanism and the HPA nonlinearity is characterized. The performance of the AF cooperative system under study is evaluated in terms of average symbol error probability (SEP), which is derived using the moment-generating function (MGF) approach, considering transmissions over Nakagami-m fading channels. Numerical results are provided and show the effects of some system parameters, such as the HPA parameters, numbers of relays, quadrature amplitude modulation (QAM) order, Nakagami parameters, on performance. © 2012 IEEE.

Performance analysis of AF cooperative systems with HPA nonlinearity in semi-blind relays

KAUST Repository

Qi, Jian; Aï ssa, Sonia; Alouini, Mohamed-Slim

2012-01-01

In this paper, dual-hop amplify-and-forward (AF) cooperative systems in the presence of high-power amplifier (HPA) nonlinearity at semi-blind relays, are investigated. Based on the modified AF cooperative system model taking into account the HPA nonlinearity, the expression for the output signal-to-noise ratio (SNR) at the destination node is derived, where the interference due to both the AF relaying mechanism and the HPA nonlinearity is characterized. The performance of the AF cooperative system under study is evaluated in terms of average symbol error probability (SEP), which is derived using the moment-generating function (MGF) approach, considering transmissions over Nakagami-m fading channels. Numerical results are provided and show the effects of some system parameters, such as the HPA parameters, numbers of relays, quadrature amplitude modulation (QAM) order, Nakagami parameters, on performance. © 2012 IEEE.

Udførelse af bygningsakustiske målinger

DEFF Research Database (Denmark)

Rasmussen, Birgit; Hoffmeyer, Dan; Olesen, Henrik S.

Denne anvisning definerer og beskriver udførelse af bygningsakustiske målinger m.m. i relation til Bygningsreglement 2015 (BR15), kapitel 6.4, Akustisk Indeklima (Trafik- og Byggestyrelen, 2016). Anvisningen er en opdatering af den første udgave fra 2008. Den nye udgave er ajourført med vejledning...... knyttet til blandt andet de nye standarder med feltmålemetoder for lydisolation. Anvisningen henvender sig primært til personer og firmaer, der udfører bygningsakustiske målinger, men vil også være et vigtigt redskab for rådgivere, udførende og leverandører i forbindelse med projektering af bygninger....... Anvisningen er også relevant for kommunerne i forbindelse med kontrol af opfyldelsen af lydbestemmelserne i bygningsreglementet....

Børns konstruktion af (bi)literacy

DEFF Research Database (Denmark)

Laursen, Helle; Daugaard, Line Møller; Østergaard, Winnie

analyser heraf. Programmet er baseret på et socialsemiotisk teorigrundlag, der indebærer, at tilegnelse af skriftsystemer ses som en proces, hvorigennem børn skaber betydning ud fra den information, der er tilgængelig for dem i de sociale fællesskaber, de indgår i (Kress 1997, 2000, Kenner 2004). For børn....... I dette paper præsenteres programmet, og de indsamlede data analyseres med særligt henblik på at identificere børnenes konstruktion af (bi)literacy og deres fortolkninger af, hvad der konsti-tuerer, og hvad der tæller som literacy...

Læremiddelevaluering af web 2.0 læremidler

DEFF Research Database (Denmark)

Falkesgaard Slot, Marie; Gissel, Stig Toke

2014-01-01

Kapitlet etablerer og afprøver et analyseapparat, der bidrager til at højne kvaliteten af de valg, som foretages af web 2.0-læremidler i danskfaget. I kapitlet fokuseres der på læremiddel evaluering af nogle af de funktioner, som typisk tilskrives web 2.0-værktøjerne, ligesom der gives eksempler på...

FEM-analyse af termiske Spændinger i Svejsning

DEFF Research Database (Denmark)

Hansen, Jan Langkjær

1997-01-01

Undersøgelser af de transiente temperaturfelter og de heraf inducerede spændinger og deformationer som følge af en stumpsøm-svejsning i plader af konstruktionsstål modelleret i henholdsvis 2D plan spændings-/tøjningstilstand og 3D er foretaget ved brug af det generelt anvendelige finite-element p......-element program ABAQUS....

Kulturbankeprojekt med udlægning af blåmuslinger produceret på langline

DEFF Research Database (Denmark)

Dolmer, Per; Christensen, Helle Torp; Christoffersen, Mads Ole

Farm systemer, men pga isskader på anlæg var dette ikke muligt. I maj 2011 blev 75 tons kompensationsopdrættede muslinger fra Skive Fjord udlagt i et 300x100 m stort område på 10-11 meters vanddybde i en tæthed på 2,5 kg m-2. I forbindelse med afhøstning af muslingerne i Skive Fjord, transport til Horsens Fjord...... hydrodynamisk model og en økologisk model. Til modelarbejdet anvendes data fra 2011 og første halvår 2012, samt data for muslingers tilvækst, som er indsamlet under projektet. På baggrund af modelanalyser anbefales det at udlægningstætheden af muslinger i området ikke overskriver 400 individer m-2. Kortlægning...... på 4-5 meters dybde. Der er gennemført 14 punktdyk med dykkerobservationer og billedoptagelse, og der er gennemført en side scan kortlægning af området. Der er ikke observeret ålegræs i området. I forbindelse med projektet er projektets resultater formidlet til Fødevareministeriet og Miljøministeriet...

Markedsføring af probiotiske fødevarer: Kan vi lære noget af Gaio og ProViva

DEFF Research Database (Denmark)

Bech-Larsen, Tino; Andersen, Lone Merete Schreiber

2004-01-01

Erfaringerne fra Gaio yoghurt (fra Arla) og ProViva frugtdrik/suppe (fra Skånemejerierne) viser, at der kan være vanskeligt at overbevise forbrugerne om probiotiske fødevarers fortrin. Både Gaio og ProViva har haft succes på deres respektive hjemmemarkeder, men ingen af produkterne har kunnet klare...... den korte tur over øresund. Med afsæt i Gaio og ProViva har MAPP og SIK undersøgt, hvordan forskellige reklame- og mediestrategier påvirker forbrugernes accept af probiotiske fødevarer. Undersøgelsen Undersøgelsen omfattede for det første en analyse af reklamer og nyhedsartikler om de to produkter...

Subregional innovation – indikatorer til optimering af den erhvervsrettede innovationspolitik

DEFF Research Database (Denmark)

Mortensen, Peter Stendahl; Graversen, Ebbe Krogh; Haase, Sanne Schioldann

områder tilgodeses bedst muligt. Desuden kan en differentieret, subregional innovations-politik give erhvervslivet bedre udbytte end en samlet regional eller national innovationspolitik. Selvom Danmark er et lille land, er der store geografiske forskelle internt i landets erhvervsstruktur og i...... virksomhedernes innovationsaktiviteter. Som resultat af en udvidelse af den danske del af CIS 2006-undersøgelsen2 med ekstra virksomheder kan der gives en betydeligt mere nuanceret karak-teristik af virksomheder helt ned på subregionalt niveau. Analysen underbygger, at de innovations-politiske tiltag, der sættes......, lokal brug af resultaterne er nødvendig. Først gennemgås de valgte målepunkter. Det primære er en opdeling i praksis- og forsknings-orientering af virksomheders innovationstilgang, hvorved der identificeres fire grupper af innovationsaktive virksomheder. Det andet er korrektion af de anvendte...

A new and efficient culture method for porcine bone marrow-derived M1- and M2-polarized macrophages.

Science.gov (United States)

Gao, Jiye; Scheenstra, Maaike R; van Dijk, Albert; Veldhuizen, Edwin J A; Haagsman, Henk P

2018-06-01

Macrophages play an important role in the innate immune system as part of the mononuclear phagocyte system (MPS). They have a pro-inflammatory signature (M1-polarized macrophages) or anti-inflammatory signature (M2-polarized macrophages) based on expression of surface receptors and secretion of cytokines. However, very little is known about the culture of macrophages from pigs and more specific about the M1 and M2 polarization in vitro. Porcine monocytes or mononuclear bone marrow cells were used to culture M1- and M2-polarized macrophages in the presence of GM-CSF and M-CSF, respectively. Surface receptor expression was measured with flow cytometry and ELISA was used to quantify cytokine secretion in response to LPS and PAM 3 CSK 4 stimulation. Human monocyte-derived macrophages were used as control. Porcine M1- and M2-polarized macrophages were cultured best using porcine GM-CSF and murine M-CSF, respectively. Cultures from bone marrow cells resulted in a higher yield M1- and M2-polarized macrophages which were better comparable to human monocyte-derived macrophages than cultures from porcine monocytes. Porcine M1-polarized macrophages displayed the characteristic fried egg shape morphology, lower CD163 expression and low IL-10 production. Porcine M2-polarized macrophages contained the spindle-like morphology, higher CD163 expression and high IL-10 production. Porcine M1- and M2-polarized macrophages can be most efficiently cultured from mononuclear bone marrow cells using porcine GM-CSF and murine M-CSF. The new culture method facilitates more refined studies of porcine macrophages in vitro, important for both porcine and human health since pigs are increasingly used as model for translational research. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Cell lineage identification and stem cell culture in a porcine model for the study of intestinal epithelial regeneration.

Directory of Open Access Journals (Sweden)

Liara M Gonzalez

Full Text Available Significant advances in intestinal stem cell biology have been made in murine models; however, anatomical and physiological differences between mice and humans limit mice as a translational model for stem cell based research. The pig has been an effective translational model, and represents a candidate species to study intestinal epithelial stem cell (IESC driven regeneration. The lack of validated reagents and epithelial culture methods is an obstacle to investigating IESC driven regeneration in a pig model. In this study, antibodies against Epithelial Adhesion Molecule 1 (EpCAM and Villin marked cells of epithelial origin. Antibodies against Proliferative Cell Nuclear Antigen (PCNA, Minichromosome Maintenance Complex 2 (MCM2, Bromodeoxyuridine (BrdU and phosphorylated Histone H3 (pH3 distinguished proliferating cells at various stages of the cell cycle. SOX9, localized to the stem/progenitor cells zone, while HOPX was restricted to the +4/'reserve' stem cell zone. Immunostaining also identified major differentiated lineages. Goblet cells were identified by Mucin 2 (MUC2; enteroendocrine cells by Chromogranin A (CGA, Gastrin and Somatostatin; and absorptive enterocytes by carbonic anhydrase II (CAII and sucrase isomaltase (SIM. Transmission electron microscopy demonstrated morphologic and sub-cellular characteristics of stem cell and differentiated intestinal epithelial cell types. Quantitative PCR gene expression analysis enabled identification of stem/progenitor cells, post mitotic cell lineages, and important growth and differentiation pathways. Additionally, a method for long-term culture of porcine crypts was developed. Biomarker characterization and development of IESC culture in the porcine model represents a foundation for translational studies of IESC-driven regeneration of the intestinal epithelium in physiology and disease.

Styring af international transfer pricing

DEFF Research Database (Denmark)

Reuther, Peter; Rossing, Christian Plesner

2011-01-01

Nærværende artikel introducerer international transfer pricing, dels som et skatteretligt fænomen og dels som et værktøj til planlægning og økonomisk styring af koncernforbundne selskaber i en multinational virksomhed (MNV). Med udgangspunkt i en case-analyse af MNV’en gives der et konkret eksempel...... på hvordan transfer pricing håndteres i praksis. Artiklens formål er at give et praktisk eksempel på at transfer pricing handler om at efterleve regler, ikke omgå dem. På baggrund af case-analysen argumenteres der for, at såvel akademikere som praktikere bør tage virksomhedens tilgang til...... international transfer pricing i betragtning, når økonomiske styringssystemer i en MNV skal anvendes til planlægning og opfølgning....

Økonomisk vurdering af energibesparende foranstaltninger

DEFF Research Database (Denmark)

Johnsen, Kjeld; Andersen, Hans Skifter

Anvisningen gennemgår detaljeret hvordan man gennemfører analyser af lønsomhed og likviditetsvirkninger som led i udarbejdelsen af det teknisk-økonomiske grundlag for beslutninger om energibesparende investeringer. Anvisningen indeholder blandt andet en række enkle skemaer, der kan lette og syste...

Måling af virksomheders administrative byrder

DEFF Research Database (Denmark)

Langsted, Lars Bo

2004-01-01

Selskabsstyrelsen har offentliggjort rapporten: "Rapport om måling af virksomhedernes administrative byrder ved årsregnskabsloven og rapport om revisionshonorar for B-virksomheder."......Selskabsstyrelsen har offentliggjort rapporten: "Rapport om måling af virksomhedernes administrative byrder ved årsregnskabsloven og rapport om revisionshonorar for B-virksomheder."...

Forekomst af alkohol, narkotika og medicin hos bilister

DEFF Research Database (Denmark)

Bernhoft, Inger Marie

2011-01-01

Inden for rammerne af det fælles-europæiske forskningsprojekt DRUID (DRiving Under the Influence of Drugs, alcohol and medicines) er forekomsten af alkohol og/eller andre stoffer blevet undersøgt i et stort antal europæiske lande, bl.a. i Danmark.......Inden for rammerne af det fælles-europæiske forskningsprojekt DRUID (DRiving Under the Influence of Drugs, alcohol and medicines) er forekomsten af alkohol og/eller andre stoffer blevet undersøgt i et stort antal europæiske lande, bl.a. i Danmark....

En tegning af æstetik 2013

DEFF Research Database (Denmark)

Buhl, Mie; Ejsing-Duun, Stine

2013-01-01

Denne artikel fremstiller skredet fra det individuelle fokus til den sociale forhandling omkring en situation, som er det nye perspektiv indenfor æstetik. Derigennem identificeres tendenser som er med til at udgøre platformen for børn og unges kulturer, hvor det handler om, at et æstetisk system er...... selvproducerende og stiller sig til rådighed for erkendelse. Disse kulturer udfolder sig i formater, som skolen ikke er gearet til at imødekomme. Som eksempel ville DrawSomething vanskeligt kunne begrundes i forhold til billedkunstfagets af ministeriet fastsatte ”fælles mål” – eller kan det? En central udfordring...... er, hvordan der skal arbejdes med æstetiske praksisformer i en nutidig læringskontekst, når børn og unges kulturer udfolder og udvikler sig i formater, der er præget af foranderlighed og flygtighed; af en uendelig skaben for nogen og med nogen og af situationer, der bebos med attituder? Vi diskuterer...

Kommunikation ved brug af mobile enheder med digital video i idræt

DEFF Research Database (Denmark)

Langagergaard Rødbro, Lise; Elbæk, Lars

2012-01-01

I artiklen redegøres kort for et projekt, som førte frem til en videoplatform, der er et bud på et ’lukket YouTube’, hvor video filmet med smartphone direkte kan uploades til, og med funktioner specifikt udviklet til skolens idrætsundervisning. Med afsæt i systemisk kommunikationsteori analyseres...... observations- og interviewda-ta for at belyse den kommunikation, der foregår ved brug af platformen. Senere diskuteres resultaterne ud fra kommunikationstrekanten, og der skitseres en model for videostøttet undervisning i idræt ud fra Batesons kommunikations- og læringsniveauer. Gennem analyser af platformens...

Reduction of IFNα and IL-10 in central nervous system and increase in peripheral IL-8 in transgenic porcine Huntington´s disease model

Czech Academy of Sciences Publication Activity Database

Kovářová, Hana; Valeková, Ivona; Jarkovská, Karla; Kotrčová, Eva; Motlík, Jan; Gadher, S. J.

2015-01-01

Roč. 78, Suppl 2 (2015), s. 10-11 ISSN 1210-7859. [Conference on Animal Models for neurodegenerative Diseases /3./. 08.11.2015-10.11.2015, Liblice] R&D Projects: GA MŠk ED2.1.00/03.0124 Institutional support: RVO:67985904 Keywords : porcine Huntington ´s disease model * IFNα * IL-10 Subject RIV: EB - Genetics ; Molecular Biology

Når ledelse af ledelse rettes mod selvet

DEFF Research Database (Denmark)

Krause-Jensen, Niels Henrik

2012-01-01

Der fokuseres på det nye selvledelsesforhold som ledelse af ledelse er med til at fremkalde. Ud fra et signalement af det nye arbejdsliv beskrives det hvordan ledelse af ledelse skal forvalte en indgriben i medarbejderens selvforhold, hvor både magt og afmagt vil gøre sig gældende....

Towards the establishment of a porcine model to study human amebiasis.

Directory of Open Access Journals (Sweden)

Fabienne Girard-Misguich

Full Text Available BACKGROUND: Entamoeba histolytica is an important parasite of the human intestine. Its life cycle is monoxenous with two stages: (i the trophozoite, growing in the intestine and (ii the cyst corresponding to the dissemination stage. The trophozoite in the intestine can live as a commensal leading to asymptomatic infection or as a tissue invasive form producing mucosal ulcers and liver abscesses. There is no animal model mimicking the whole disease cycle. Most of the biological information on E. histolytica has been obtained from trophozoite adapted to axenic culture. The reproduction of intestinal amebiasis in an animal model is difficult while for liver amebiasis there are well-described rodent models. During this study, we worked on the assessment of pigs as a new potential model to study amebiasis. METHODOLOGY/PRINCIPAL FINDINGS: We first co-cultured trophozoites of E. histolytica with porcine colonic fragments and observed a disruption of the mucosal architecture. Then, we showed that outbred pigs can be used to reproduce some lesions associated with human amebiasis. A detailed analysis was performed using a washed closed-jejunal loops model. In loops inoculated with virulent amebas a severe acute ulcerative jejunitis was observed with large hemorrhagic lesions 14 days post-inoculation associated with the presence of the trophozoites in the depth of the mucosa in two out four animals. Furthermore, typical large sized hepatic abscesses were observed in the liver of one animal 7 days post-injection in the portal vein and the liver parenchyma. CONCLUSIONS: The pig model could help with simultaneously studying intestinal and extraintestinal lesion development.

Overmæt af islam kontra danskhed

DEFF Research Database (Denmark)

Khawaja, Iram

2013-01-01

Debatindlæg der retter fokus på at, islam og muslimer konstrueres som modbillede til alt det, som danskhed repræsenterer, og at man i denne forbindelse glemmer at definitionen af danskhed gennem negationen af islam sker på bekostning af de mange muslimske borgere, der har skabt en identitet og...... selvforståelse som danske muslimer. Der tales her om de mange mennesker, der har skabt en tilværelse ved at sammentænke danskhed og islam i et forsøg på at overskride kontrasten imellem et fremmed ”dem” og et dansk ”os”. Denne gruppe udgør størstedelen af de herboende unge, voksne og børn, som ikke forstår...

Udtrækning af 10 mm ankerbolte

DEFF Research Database (Denmark)

Hansen, Lars Pilegaard

Denne rapport beskriver forsøg med udtrækning af 10 mm ankerbolte udført af Laboratoriet for Bærende Konstruktioner, Instituttet for Bygningsteknik, Aalborg Universitet. Forsøgene blev udført d. 19. og 21. juni 2001. Forsøgene blev udført dels på Hjørring Sygehus og dels på Laboratoriet.......Denne rapport beskriver forsøg med udtrækning af 10 mm ankerbolte udført af Laboratoriet for Bærende Konstruktioner, Instituttet for Bygningsteknik, Aalborg Universitet. Forsøgene blev udført d. 19. og 21. juni 2001. Forsøgene blev udført dels på Hjørring Sygehus og dels på Laboratoriet....

First identification of porcine parvovirus 6 in North America by viral metagenomic sequencing of serum from pigs infected with porcine reproductive and respiratory syndrome virus.

Science.gov (United States)

Schirtzinger, Erin E; Suddith, Andrew W; Hause, Benjamin M; Hesse, Richard A

2015-10-16

Currently, eight species in four genera of parvovirus have been described that infect swine. These include ungulate protoparvovirus 1 (classical porcine parvovirus, PPV), ungulate tetraparvovirus 2 (PPV3), ungulate tetraparvovirus 3 (which includes PPV2, porcine hokovirus, porcine partetravirus and porcine PARV4), ungulate copiparvovirus 2 (which includes PPV4 and PPV5), ungulate bocaparvovirus 2 (which includes porcine bocavirus 1, 2 and 6), ungulate bocaparvovirus 3 (porcine bocavirus 5), ungulate bocaparvovirus 4 (porcine bocavirus 7) and ungulate bocaparvovirus 5 (porcine bocavirus 3, 4-1 and 4-2). PPV6, the most recently described porcine parvovirus, was first identified in China in late 2014 in aborted pig fetuses. Prevalence of PPV6 in China was found to be similar in finishing age pigs from farms with and without evidence of swine reproductive failure. Porcine parvovirus 6 (PPV6) was detected by sequence-independent single primer amplification (SISPA) and confirmed by overlapping and real-time PCR in the serum of porcine reproductive and respiratory virus (PRRSv) positive samples. Seven nearly complete genomes of PPV6 were identified in PRRSv genotype 2 positive serum samples submitted to state veterinary diagnostic laboratories in 2014. Further testing using overlapping and real-time PCR determined PPV6 to be present in 13.2 % of the serums tested. Additionally, PPV6 was present in samples from all of the geographic locations sampled encompassing nine states in the United States and one state in Mexico. The presence of PPV6 in serum indicates that the PPV6 infection is disseminated and not localized to a specific tissue type. Alignments of the near full length genomes, NS1, and capsid genes identified one of the five PPV6 isolates from China (98.6-99.5 % identity with the North American strains) to be the North American strains nearest relative. These results are the first to report the presence of PPV6 in North America and demonstrate that the virus is

Undersøgelse af kommunernes administration af bygningsreglementets elevatorkrav

DEFF Research Database (Denmark)

Grangaard, Sidse

Denne rapport undersøger kommunernes praksis omkring administrationen af bygningsreglementets krav om elevator ved nybyggeri og ombygning. Blandt de 26 kommuner, der har deltaget i undersøgelsen, var der generelt konsensus om at stille krav om elevator ved nybyggeri. Kommunerne modtager kun meget...

Overvågning af fugle 1997-98, resultater fra feltstationerne

DEFF Research Database (Denmark)

Vejler, i alt ca. 5.500 ha. Feltstationen blev oprettet i 1978, og siden har observatørerne registreret fuglelivet i Vejlerne og de omgivende arealer foruden vandstand, saltholdighed og drift af området. Tipperne ligger i den sydlige del af Ringkøbing Fjord. Fuglelivet blev fredet i 1898. Reservatet blev...... en samlet fredning af Vadehavet, omfatter øen Langli på 80 ha og vandarealer på i alt ca. 8.000 ha. Øen består af klitter og salte strandenge. Vandområdet er præget af tidevand, hvor vidtstrakte sand- og mudderflader og spredte muslingebanker er synlige ved lavvande. Vorsø ligger i Horsens Fjord...... saltholdighed i Ringkøbing Fjord bevirker, at vandplanter vokser dårligt eller forsvinder. Vandplanter udgør en væsentlig del af føden for visse andefugle og kan derfor have stor betydning for antallet af rastende fugle. Der blev registreret lave antal af knortegå, gråand, pibeand og krikand. Af blishøne blev...

Optimalt design af fødevarer

DEFF Research Database (Denmark)

Bech, Anne C.; Engelund, Erling; Juhl, Hans Jørn

1993-01-01

Udformningen af fødevarer opfattes som et designproblem, det består i at omsætte markedskrav, belyst gennem markedsanalyser, til egenskaber ved de fremstillede produkter under hensyntagen til de dermed forbundne omkostninger. Denne oversættelse af forbrugerønsker antages at kunne ske effektivt ge...

Improvement of specific growth rate of Pichia pastoris for effective porcine interferon-α production with an on-line model-based glycerol feeding strategy.

Science.gov (United States)

Gao, Min-Jie; Zheng, Zhi-Yong; Wu, Jian-Rong; Dong, Shi-Juan; Li, Zhen; Jin, Hu; Zhan, Xiao-Bei; Lin, Chi-Chung

2012-02-01

Effective expression of porcine interferon-α (pIFN-α) with recombinant Pichia pastoris was conducted in a bench-scale fermentor. The influence of the glycerol feeding strategy on the specific growth rate and protein production was investigated. The traditional DO-stat feeding strategy led to very low cell growth rate resulting in low dry cell weight (DCW) of about 90 g/L during the subsequent induction phase. The previously reported Artificial Neural Network Pattern Recognition (ANNPR) model-based glycerol feeding strategy improved the cell density to 120 g DCW/L, while the specific growth rate decreased from 0.15 to 0.18 to 0.03-0.08 h(-1) during the last 10 h of the glycerol feeding stage leading to a variation of the porcine interferon-α production, as the glycerol feeding scheme had a significant effect on the induction phase. This problem was resolved by an improved ANNPR model-based feeding strategy to maintain the specific growth rate above 0.11 h(-1). With this feeding strategy, the pIFN-α concentration reached a level of 1.43 g/L, more than 1.5-fold higher than that obtained with the previously adopted feeding strategy. Our results showed that increasing the specific growth rate favored the target protein production and the glycerol feeding methods directly influenced the induction stage. Consequently, higher cell density and specific growth rate as well as effective porcine interferon-α production have been achieved by our novel glycerol feeding strategy.

Protecting your files on the AFS file system

CERN Multimedia

2011-01-01

The Andrew File System is a world-wide distributed file system linking hundreds of universities and organizations, including CERN. Files can be accessed from anywhere, via dedicated AFS client programs or via web interfaces that export the file contents on the web. Due to the ease of access to AFS it is of utmost importance to properly protect access to sensitive data in AFS. As the use of AFS access control mechanisms is not obvious to all users, passwords, private SSH keys or certificates have been exposed in the past. In one specific instance, this also led to bad publicity due to a journalist accessing supposedly "private" AFS folders (SonntagsZeitung 2009/11/08). This problem does not only affect the individual user but also has a bad impact on CERN's reputation when it comes to IT security. Therefore, all departments and LHC experiments agreed in April 2010 to apply more stringent folder protections to all AFS user folders. The goal of this data protection policy is to assist users in...

Bagsideidræt ramt af skandale

DEFF Research Database (Denmark)

Elbæk, Lars

2008-01-01

Ekspert rystet over sikkerheden - idrætsgren udelukket - redaktør sporløst forsvundet Fyens Bagside Idræt (FBI) er rystet af sin første skandale. Det drejer sig om sikkerheden i den af forbundet indtil i går anerkendte disciplin trampolinbold, hvor flere børn på samme tid hopper i trampolin, mens...... de forsøger at undgå at ramme bolde på samme trampolin. - Det er direkte uansvarligt og jeg er rystet, siger lektor Lars Elbæk fra Institut for Idræt og Biomekanik på Syddansk Universitet. - I overtræder simpelthen samtlige sikkerhedsregler for, hvordan man skal undgå skader på en trampolin...... skader, der opstår i havetrampoliner, sker på baggrund af uhensigtsmæssig brug, siger Lars Elbæk, som har opstillet 12 sikkerhedsanbefalinger, der blandt andet kan ses på www.trampolin.dk . En af disse foranstaltninger er, at kun én person ad gangen hopper i trampolinen: - 75 procent af alle...

Similarities in the immunoglobulin response and VH gene usage in rhesus monkeys and humans exposed to porcine hepatocytes

Directory of Open Access Journals (Sweden)

Borie Dominic C

2006-03-01

Full Text Available Abstract Background The use of porcine cells and organs as a source of xenografts for human patients would vastly increase the donor pool; however, both humans and Old World primates vigorously reject pig tissues due to xenoantibodies that react with the polysaccharide galactose α (1,3 galactose (αGal present on the surface of many porcine cells. We previously examined the xenoantibody response in patients exposed to porcine hepatocytes via treatment(s with bioartficial liver devices (BALs, composed of porcine cells in a support matrix. We determined that xenoantibodies in BAL-treated patients are predominantly directed at porcine αGal carbohydrate epitopes, and are encoded by a small number of germline heavy chain variable region (VH immunoglobulin genes. The studies described in this manuscript were designed to identify whether the xenoantibody responses and the IgVH genes encoding antibodies to porcine hepatocytes in non-human primates used as preclinical models are similar to those in humans. Adult non-immunosuppressed rhesus monkeys (Macaca mulatta were injected intra-portally with porcine hepatocytes or heterotopically transplanted with a porcine liver lobe. Peripheral blood leukocytes and serum were obtained prior to and at multiple time points after exposure, and the immune response was characterized, using ELISA to evaluate the levels and specificities of circulating xenoantibodies, and the production of cDNA libraries to determine the genes used by B cells to encode those antibodies. Results Xenoantibodies produced following exposure to isolated hepatocytes and solid organ liver grafts were predominantly encoded by genes in the VH3 family, with a minor contribution from the VH4 family. Immunoglobulin heavy-chain gene (VH cDNA library screening and gene sequencing of IgM libraries identified the genes as most closely-related to the IGHV3-11 and IGHV4-59 germline progenitors. One of the genes most similar to IGHV3-11, VH3-11cyno, has

Use of porcine vaginal tissue ex-vivo to model environmental effects on vaginal mucosa to toxic shock syndrome toxin-1

Energy Technology Data Exchange (ETDEWEB)

Davis, Catherine C.; Baccam, Mekhine [Feminine Care Global Product Stewardship, 6110 Center Hill Road, The Procter and Gamble Company, Cincinnati, OH 45224 (United States); Mantz, Mary J. [Dows Institute for Dental Research, The University of Iowa, Iowa City, IA 52242 (United States); Osborn, Thomas W.; Hill, Donna R. [Feminine Care Product Development, 6110 Center Hill Road, The Procter and Gamble Company, Cincinnati, OH 45224 (United States); Squier, Christopher A. [Dows Institute for Dental Research, The University of Iowa, Iowa City, IA 52242 (United States)

2014-01-15

Menstrual toxic shock syndrome (mTSS) is a rare, recognizable, and treatable disease that has been associated with tampon use epidemiologically. It involves a confluence of microbial risk factors (Staphylococcus aureus strains that produce the superantigen—TSST-1), as well as environmental characteristics of the vaginal ecosystem during menstruation and host susceptibility factors. This paper describes a series of experiments using the well-characterized model of porcine vaginal mucosa ex-vivo to assess the effect of these factors associated with tampon use on the permeability of the mucosa. The flux of radiolabeled TSST-1 and tritiated water ({sup 3}H{sub 2}O) through porcine vaginal mucosa was determined at various temperatures, after mechanical disruption of the epithelial surface by tape stripping, after treatment with surfactants or other compounds, and in the presence of microbial virulence factors. Elevated temperatures (42, 47 and 52 °C) did not significantly increase flux of {sup 3}H{sub 2}O. Stripping of the epithelial layers significantly increased the flux of labeled toxin in a dose-dependent manner. Addition of benzalkonium chloride (0.1 and 0.5%) and glycerol (4%) significantly increased the flux of {sup 3}H{sub 2}O but sodium lauryl sulfate at any concentration tested did not. The flux of the labeled toxin was significantly increased in the presence of benzalkonium chloride but not Pluronic® L92 and Tween 20 and significantly increased with addition of α-hemolysin but not endotoxin. These results show that the permeability of porcine vagina ex-vivo to labeled toxin or water can be used to evaluate changes to the vaginal environment and modifications in tampon materials, and thus aid in risk assessment. - Highlights: • Model assessed local effects of tampon use on vaginal mucosa. • Risks were evaluated using two tracers to assess permeability in an ex vivo model. • Mechanical damage to the epithelial surface increased tracer penetration. �

Use of porcine vaginal tissue ex-vivo to model environmental effects on vaginal mucosa to toxic shock syndrome toxin-1

International Nuclear Information System (INIS)

Davis, Catherine C.; Baccam, Mekhine; Mantz, Mary J.; Osborn, Thomas W.; Hill, Donna R.; Squier, Christopher A.

2014-01-01

Menstrual toxic shock syndrome (mTSS) is a rare, recognizable, and treatable disease that has been associated with tampon use epidemiologically. It involves a confluence of microbial risk factors (Staphylococcus aureus strains that produce the superantigen—TSST-1), as well as environmental characteristics of the vaginal ecosystem during menstruation and host susceptibility factors. This paper describes a series of experiments using the well-characterized model of porcine vaginal mucosa ex-vivo to assess the effect of these factors associated with tampon use on the permeability of the mucosa. The flux of radiolabeled TSST-1 and tritiated water ( 3 H 2 O) through porcine vaginal mucosa was determined at various temperatures, after mechanical disruption of the epithelial surface by tape stripping, after treatment with surfactants or other compounds, and in the presence of microbial virulence factors. Elevated temperatures (42, 47 and 52 °C) did not significantly increase flux of 3 H 2 O. Stripping of the epithelial layers significantly increased the flux of labeled toxin in a dose-dependent manner. Addition of benzalkonium chloride (0.1 and 0.5%) and glycerol (4%) significantly increased the flux of 3 H 2 O but sodium lauryl sulfate at any concentration tested did not. The flux of the labeled toxin was significantly increased in the presence of benzalkonium chloride but not Pluronic® L92 and Tween 20 and significantly increased with addition of α-hemolysin but not endotoxin. These results show that the permeability of porcine vagina ex-vivo to labeled toxin or water can be used to evaluate changes to the vaginal environment and modifications in tampon materials, and thus aid in risk assessment. - Highlights: • Model assessed local effects of tampon use on vaginal mucosa. • Risks were evaluated using two tracers to assess permeability in an ex vivo model. • Mechanical damage to the epithelial surface increased tracer penetration. • Surfactants increased

An ex vivo porcine skin model to evaluate pressure-reducing devices of different mechanical properties used for pressure ulcer prevention.

Science.gov (United States)

Yeung, Ching-Yan C; Holmes, David F; Thomason, Helen A; Stephenson, Christian; Derby, Brian; Hardman, Matthew J

2016-11-01

Pressure ulcers are complex wounds caused by pressure- and shear-induced trauma to skin and underlying tissues. Pressure-reducing devices, such as dressings, have been shown to successfully reduce pressure ulcer incidence, when used in adjunct to pressure ulcer preventative care. While pressure-reducing devices are available in a range of materials, with differing mechanical properties, understanding of how a material's mechanical properties will influence clinical efficacy remains limited. The aim of this study was to establish a standardized ex vivo model to allow comparison of the cell protection potential of two gel-like pressure-reducing devices with differing mechanical properties (elastic moduli of 77 vs. 35 kPa). The devices also displayed differing energy dissipation under compressive loading, and resisted strain differently under constant load in compressive creep tests. To evaluate biological efficacy we employed a new ex vivo porcine skin model, with a confirmed elastic moduli closely matching that of human skin (113 vs. 119 kPa, respectively). Static loads up to 20 kPa were applied to porcine skin ex vivo with subsequent evaluation of pressure-induced cell death and cytokine release. Pressure application alone increased the percentage of epidermal apoptotic cells from less than 2% to over 40%, and increased cellular secretion of the pro-inflammatory cytokine TNF-alpha. Co-application of a pressure-reducing device significantly reduced both cellular apoptosis and cytokine production, protecting against cellular damage. These data reveal new insight into the relationship between mechanical properties of pressure-reducing devices and their biological effects. After appropriate validation of these results in clinical pressure ulcer prevention with all tissue layers present between the bony prominence and external surface, this ex vivo porcine skin model could be widely employed to optimize design and evaluation of devices aimed at reducing pressure

Bestemmelse af spormetaller i organisk materiale

DEFF Research Database (Denmark)

Tjell, Jens Christian; Palfeldt, Kirsten

Behovet for pålidelige analysemetoder til bestemmelse af spormetalindhold i organiske materialer, f.eks. planter, slam og dyrevæv er stigende på grund af den stigende erkendelse af disse stoffers betydning for organismers fysiologi, både hvor disse stoffer optræder i uønskede koncentrationer som...... der over de sidste år udviklet en analyseprocedure hvorefter det er muligt at analysere for metallerne Mn, Cu, Ni, Co, Cr, Zn, Cd og Pb i planter, dyrevæv, jord, slam og andre materialer med standardafvigelser på mellem 5 og 10%. Der gøres rede for denne metode....

Sproget afhænger af stedet

DEFF Research Database (Denmark)

Monka, Malene

2014-01-01

I artiklen præsenteres resultaterne af et ph.d.-projekt ved Nordisk Forskningsinstitut ved Københavns Universitetet, der undersøger sammenhænge mellem sted og sprogforandring. Projektet er en undersøgelse af sprogforandring i virkelig tid, hvor gamle og nye optagelser med 23 informanter fra tre j...

Overvågning af influenza A virus i svin - Slutrapport 2015

DEFF Research Database (Denmark)

Krog, Jesper Schak; Hjulsager, Charlotte Kristiane; Larsen, Lars Erik

Denne rapport beskriver resultaterne af overvågning af influenza i svin i Danmark i 2015 og sammenholder resultaterne med dem fra de foregående år og udenlandske studier. Laboratorieundersøgelser og databehandling er udført på DTU Veterinærinstituttet. Indledende screening af prøver for tilstedev......Denne rapport beskriver resultaterne af overvågning af influenza i svin i Danmark i 2015 og sammenholder resultaterne med dem fra de foregående år og udenlandske studier. Laboratorieundersøgelser og databehandling er udført på DTU Veterinærinstituttet. Indledende screening af prøver...... for tilstedeværelsen af influenza A virus er betalt af indsenderne, mens de øvrige analyser er finansieret af FVST’s overvågningsprogram. Slutrapporten er den endelige opgørelse af analyserede indsendelser for det pågældende år. Der kan være mindre afvigelser mellem slutrapport og kvartalsrapporterne....

Mechanics of fresh, frozen-thawed and heated porcine liver tissue.

Science.gov (United States)

Wex, Cora; Stoll, Anke; Fröhlich, Marlen; Arndt, Susann; Lippert, Hans

2014-06-01

For a better understanding of the effects of thermally altered soft tissue, the biothermomechanics of these tissues need to be studied. Without the knowledge of the underlying physical processes and the parameters that can be controlled clinically, thermal treatment of cancerous hepatic tissue or the preservation of liver grafts are based primarily on trial and error. Thus, this study is concerned with the investigation of the influence of temperature on the rheological properties and the histological properties of porcine liver. Heating previously cooled porcine liver tissue above 40 °C leads to significant, irreversible stiffness changes observed in the amplitude sweep. The increase of the complex shear module of healthy porcine liver from room temperature to 70 °C is approximately 9-fold. Comparing the temperatures -20 °C and 20 °C, no significant difference of the mechanical properties was observed. Furthermore, there is a strong relation between the mechanical and histological properties of the porcine liver. Temperatures above 40 °C destroy the collagen matrix within the liver tissue. This results in the alteration of the biomechanical properties. The time-temperature superposition principle is applied to generate temperature-dependent shift factors that can be described by a two-part exponential function model with an inflection temperature of 45 °C. Tumor ablation techniques such as heating or freezing have a significant influence on the histology of liver tissue. However, only for temperatures above body temperature an influence on the mechanical properties of hepatic tissues was noticeable. Freezing up to -20 °C did not affect the liver mechanics.

Spatial clustering of porcine cysticercosis in Mbulu district, northern Tanzania.

Directory of Open Access Journals (Sweden)

Helena A Ngowi

Full Text Available BACKGROUND: Porcine cysticercosis is caused by a zoonotic tapeworm, Taenia solium, which causes serious disease syndromes in human. Effective control of the parasite requires knowledge on the burden and pattern of the infections in order to properly direct limited resources. The objective of this study was to establish the spatial distribution of porcine cysticercosis in Mbulu district, northern Tanzania, to guide control strategies. METHODOLOGY/PRINCIPAL FINDINGS: This study is a secondary analysis of data collected during the baseline and follow-up periods of a randomized community trial aiming at reducing the incidence rate of porcine cysticercosis through an educational program. At baseline, 784 randomly selected pig-keeping households located in 42 villages in 14 wards were included. Lingual examination of indigenous pigs aged 2-12 (median 8 months, one randomly selected from each household, were conducted. Data from the control group of the randomized trial that included 21 of the 42 villages were used for the incidence study. A total of 295 pig-keeping households were provided with sentinel pigs (one each and reassessed for cysticercosis incidence once or twice for 2-9 (median 4 months using lingual examination and antigen ELISA. Prevalence of porcine cysticercosis was computed in Epi Info 3.5. The prevalence and incidence of porcine cysticercosis were mapped at household level using ArcView 3.2. K functions were computed in R software to assess general clustering of porcine cysticercosis. Spatial scan statistics were computed in SatScan to identify local clusters of the infection. The overall prevalence of porcine cysticercosis was 7.3% (95% CI: 5.6, 9.4; n = 784. The K functions revealed a significant overall clustering of porcine cysticercosis incidence for all distances between 600 m and 5 km from a randomly chosen case household based on Ag-ELISA. Lingual examination revealed clustering from 650 m to 6 km and between 7.5 and 10 km

Tuning the Music: Acoustic Force Spectroscopy (AFS) 2.0

NARCIS (Netherlands)

Kamsma, D.; Creyghton, R.; Sitters, G.; Wuite, G.J.L.; Peterman, E.J.G.

2016-01-01

AFS is a recently introduced high-throughput single-molecule technique that allows studying structural and mechanochemical properties of many biomolecules in parallel. To further improve the method, we developed a modelling tool to optimize the layer thicknesses, and a calibration method to

Anmeldelser af litteratur om den græsk-romerske oldtid

DEFF Research Database (Denmark)

Krasilnikoff, Jens

2007-01-01

Anmeldelse af Neville Morley, "Trade in Classical Antiquity". Key Themes in Ancient History. Cambridge UP, 2007. p.22. Anmeldelse af J. Bingen, "Hellenistic Egypt. Monarchy, Society, economy, Culture", Edinburgh UP, 2007, pp.22-23. Anmeldelse af C. Koehn, "Krieg-Diplomatie-Ideologie. Zur Ausenpol......Anmeldelse af Neville Morley, "Trade in Classical Antiquity". Key Themes in Ancient History. Cambridge UP, 2007. p.22. Anmeldelse af J. Bingen, "Hellenistic Egypt. Monarchy, Society, economy, Culture", Edinburgh UP, 2007, pp.22-23. Anmeldelse af C. Koehn, "Krieg-Diplomatie-Ideologie. Zur...... Ausenpolitik hellenistischer Mittelstaaten", Franz Steiner 2007, pp.24-25. Anmeldelse af A. Kühr,"Als Kadmos nach Boiotien kam. Polis und ethnos im Spiegel thebanischer Gründungsmythen", Franz Steiner 2007, p. 25. P. Low, "Interstate relations in Classical Greece. Morality and Power", Cambridge UP 2007, pp. 26...

Melatonin Does Not Affect Oxidative/Inflammatory Biomarkers in a Closed-Chest Porcine Model of Acute Myocardial Infarction

DEFF Research Database (Denmark)

Halladin, Natalie L.; Ekelof, Sarah; Jensen, Svend Eggert

2014-01-01

Aim: To test whether melatonin reduces oxidative and inflammatory biomarkers in a closed-chest porcine model of acute myocardial infarction. Materials and Methods: Twenty pigs were randomized to receive a total dosage of 200 mg (0.4 mg/ml) of melatonin, or placebo immediately prior to reperfusion....... There was an increase in hs-TnT, but no significant difference between the melatonin-treated and placebo-treated groups. There were no significant differences in development of any of the circulating plasma markers between the two groups. Conclusion: Melatonin treatment did not result in reduction of inflammatory...

Udforskning af dialoger om et regnehul

DEFF Research Database (Denmark)

Lindenskov, Lena

2007-01-01

Med introduktionen af det danske begreb 'regnehuller' om matematikvanskeligheder bliver det muligt at udforske dialoger i klasserummet med brug af værktøjer fra Augusto Boals Forumteater. Principperne i Boals tre faser præsenteres som værktøjer for læreres refleksive praksis. Ud fra datamateriale...

Revisionsklienters anvendelse af serviceleverandører

DEFF Research Database (Denmark)

Kjersgaard, Lisbeth; Kiertzner, Lars

2011-01-01

Artiklen præsenterer det overordnede indhold af den ajourførte ISA 402 om revisionsmæssige overvejelser ved revision af regnskaber for virk somheder, der anvender service-leverandører. Artiklen er opbygget efter de tre grundlæggende elementer i revisionsprocessen - planlægning, udførelse og rappo...

Porcine SLITRK1

DEFF Research Database (Denmark)

Larsen, Knud Erik; Momeni, Jamal; Farajzadeh, Leila

2014-01-01

The membrane protein SLITRK1 functions as a developmentally regulated stimulator of neurite outgrowth and variants in this gene have been implicated in Tourette syndrome. In the current study we have cloned and characterized the porcine SLITRK1 gene. The genomic organization of SLITRK1 lacks...

Porcine Pluripotent Stem Cells Derived from IVF Embryos Contribute to Chimeric Development In Vivo.

Directory of Open Access Journals (Sweden)

Binghua Xue

Full Text Available Although the pig is considered an important model of human disease and an ideal animal for the preclinical testing of cell transplantation, the utility of this model has been hampered by a lack of genuine porcine embryonic stem cells. Here, we derived a porcine pluripotent stem cell (pPSC line from day 5.5 blastocysts in a newly developed culture system based on MXV medium and a 5% oxygen atmosphere. The pPSCs had been passaged more than 75 times over two years, and the morphology of the colony was similar to that of human embryonic stem cells. Characterization and assessment showed that the pPSCs were alkaline phosphatase (AKP positive, possessed normal karyotypes and expressed classic pluripotent markers, including OCT4, SOX2 and NANOG. In vitro differentiation through embryonic body formation and in vivo differentiation via teratoma formation in nude mice demonstrated that the pPSCs could differentiate into cells of the three germ layers. The pPSCs transfected with fuw-DsRed (pPSC-FDs could be passaged with a stable expression of both DsRed and pluripotent markers. Notably, when pPSC-FDs were used as donor cells for somatic nuclear transfer, 11.52% of the reconstructed embryos developed into blastocysts, which was not significantly different from that of the reconstructed embryos derived from porcine embryonic fibroblasts. When pPSC-FDs were injected into day 4.5 blastocysts, they became involved in the in vitro embryonic development and contributed to the viscera of foetuses at day 50 of pregnancy as well as the developed placenta after the chimeric blastocysts were transferred into recipients. These findings indicated that the pPSCs were porcine pluripotent cells; that this would be a useful cell line for porcine genetic engineering and a valuable cell line for clarifying the molecular mechanism of pluripotency regulation in pigs.

Treatment of severe porcine tracheomalacia with a 3-dimensionally printed, bioresorbable, external airway splint

Science.gov (United States)

Zopf, David A.; Flanagan, Colleen L.; Wheeler, Matthew; Hollister, Scott J.; Green, Glenn E.

2015-01-01

Importance The study demonstrates an application for 3-dimensional (3D) printing that may serve as an effective intervention for severe tracheobronchomalacia. Objective A novel 3D printed, bioresorbable airway splint is tested for efficacy in extending survival in an animal model of severe, life-threatening tracheobronchomalacia. Participants Evaluation of an external airway splint for severe, life-threatening tracheobronchomalacia in a porcine animal model. Setting Multi-institutional and multidisciplinary collaboration between biomedical engineering laboratories and an academic animal surgery center. Interventions Experimental analysis of a 3D printed, bioresorbable airway splint is assessed in a porcine animal model of life-threatening tracheobronchomalacia. The open-cylindrical, bellow shaped porous polycaprolactone splint is placed externally and designed to suspend the underlying collapsed airway. Control animals (n=3) undergoing tracheal cartilage division and inner tracheal lumen dissociation and experimental animals (n=3) receiving the same model with overlying placement of the newly developed airway splint were evaluated. Main Outcomes and Measures An animal model for severe, life-threatening tracheobronchomalacia is proposed. Complete or near complete tracheal lumen collapse was observed in each animal with resolution of symptoms in all of the experimental animals after splint placement. Using our severe tracheobronchomalacia animal model, survival was significantly longer in duration in the experimental group receiving the airway splint after model creation when compared to model creation alone (p = 0.0495). Mortality in the experimental group was related to infection. Conclusions A multidisciplinary effort producing a CAD/CAM, bioresorbable tracheobronchial splint was tested in a porcine model of severe tracheomalacia and was found to extend survival. PMID:24232078

3D thoracoscopic ultrasound volume measurement validation in an ex vivo and in vivo porcine model of lung tumours

International Nuclear Information System (INIS)

Hornblower, V D M; Yu, E; Fenster, A; Battista, J J; Malthaner, R A

2007-01-01

The purpose of this study was to validate the accuracy and reliability of volume measurements obtained using three-dimensional (3D) thoracoscopic ultrasound (US) imaging. Artificial 'tumours' were created by injecting a liquid agar mixture into spherical moulds of known volume. Once solidified, the 'tumours' were implanted into the lung tissue in both a porcine lung sample ex vivo and a surgical porcine model in vivo. 3D US images were created by mechanically rotating the thoracoscopic ultrasound probe about its long axis while the transducer was maintained in close contact with the tissue. Volume measurements were made by one observer using the ultrasound images and a manual-radial segmentation technique and these were compared with the known volumes of the agar. In vitro measurements had average accuracy and precision of 4.76% and 1.77%, respectively; in vivo measurements had average accuracy and precision of 8.18% and 1.75%, respectively. The 3D thoracoscopic ultrasound can be used to accurately and reproducibly measure 'tumour' volumes both in vivo and ex vivo

3D thoracoscopic ultrasound volume measurement validation in an ex vivo and in vivo porcine model of lung tumours

Energy Technology Data Exchange (ETDEWEB)

Hornblower, V D M [Canadian Surgical Technologies and Advanced Robotics, London, Ontario (Canada); Yu, E [Canadian Surgical Technologies and Advanced Robotics, London, Ontario (Canada); Fenster, A [Canadian Surgical Technologies and Advanced Robotics, London, Ontario (Canada); Battista, J J [Canadian Surgical Technologies and Advanced Robotics, London, Ontario (Canada); Malthaner, R A [Canadian Surgical Technologies and Advanced Robotics, London, Ontario (Canada)

2007-01-07

The purpose of this study was to validate the accuracy and reliability of volume measurements obtained using three-dimensional (3D) thoracoscopic ultrasound (US) imaging. Artificial 'tumours' were created by injecting a liquid agar mixture into spherical moulds of known volume. Once solidified, the 'tumours' were implanted into the lung tissue in both a porcine lung sample ex vivo and a surgical porcine model in vivo. 3D US images were created by mechanically rotating the thoracoscopic ultrasound probe about its long axis while the transducer was maintained in close contact with the tissue. Volume measurements were made by one observer using the ultrasound images and a manual-radial segmentation technique and these were compared with the known volumes of the agar. In vitro measurements had average accuracy and precision of 4.76% and 1.77%, respectively; in vivo measurements had average accuracy and precision of 8.18% and 1.75%, respectively. The 3D thoracoscopic ultrasound can be used to accurately and reproducibly measure 'tumour' volumes both in vivo and ex vivo.

Fem Dewey-begreber til analyse af Facilitated Work Based Learning

DEFF Research Database (Denmark)

Thomassen, Anja Overgaard

teoretiske ramme er helt overvejende baseret på John Deweys (1859-1952) pragmatisme og herudfra er der udviklet modeller, som anvendes i analysen af de empiriske data. Det fremlægges i paperet, hvorledes disse modeller er blevet udviklet og anvendt i ph.d.-afhandlingen, og hvorledes de centrale fem begreber...

Kan man ventilere sig ud af problemer med forhøjet indhold af PCB i indeluften?

DEFF Research Database (Denmark)

Lyng, Nadja

2016-01-01

Vi ser stadig, at byggematerialer fra perioden i nogle tilfælde er årsag til forhøjede koncentrationer af PCB i indeklimaet. Sundhedsstyrelsen har derfor fastsat en vejledende aktionsgrænse på 300 ng/ m ³. Ved koncentrationer over denne grænse anbefales midlertidig afhjælpning som øget rengøring og...... ventilation eller udluftning implementeret straks. Er disse tiltag ikke tilstrækkelige til at nedbringe koncentrationen, anbefales det uden unødig forsinkelse at nedbringe koncentrationen ved kildefjernelse eller indkapsling. Ventilation har betydning for PCB-koncentrationen i indeluften, men kan ventilation...... forurenede lokaler. Da PCB-koncentrationen afhænger af temperaturen, er alle koncentrationer korrigeret for temperatur for at kunne vurdere effekten af ventilation alene....

Functional verification of a porcine myostatin propeptide mutant.

Science.gov (United States)

Ma, Dezun; Jiang, Shengwang; Gao, Pengfei; Qian, Lili; Wang, Qingqing; Cai, Chunbo; Xiao, Gaojun; Yang, Jinzeng; Cui, Wentao

2015-10-01

Myostatin is a member of TGF-β superfamily that acts as a key negative regulator in development and growth of embryonic and postnatal muscles. In this study, the inhibitory activities of recombinant porcine myostatin propeptide and its mutated form (at the cleavage site of metalloproteinases of BMP-1/TLD family) against murine myostatin was evaluated in vivo by intraperitoneal injection into mice. Results showed that both wild type and mutated form of porcine propeptide significantly inhibited myostatin activity in vivo. The average body weight of mice receiving wild type propeptide or its mutated form increased by 12.5 % and 24.14%, respectively, compared to mice injected with PBS, implying that the in vivo efficacy of porcine propeptide mutant is greater than its wild type propeptide. Transgenic mice expressing porcine myostatin propeptide mutant were generated to further verify the results obtained from mice injected with recombinant porcine propeptide mutant. Compared with wild type (non-transgenic) mice, relative weight of gastrocnemius, rectusfemoris, and tibialis anterior increased by 22.14 %, 34.13 %, 25.37%, respectively, in transgenic male mice, and by 19.90 %, 42.47 %, 45.61%, respectively, in transgenic female mice. Our data also demonstrated that the mechanism by which muscle growth enhancement is achieved by these propeptides is due to an increase in fiber sizes, not by an increase in number of fiber cells.

Testing Danegaptide Effects on Kidney Function after Ischemia/Reperfusion Injury in a New Porcine Two Week Model.

Directory of Open Access Journals (Sweden)

Chris Amdisen

Full Text Available Ischemia/reperfusion injury (I/R-I is a leading cause of acute kidney injury (AKI and is associated with increased mortality. Danegaptide is a selective modifier of the gap junction protein connexion 43. It has cytoprotective as well as anti-arrhythmic properties and has been shown to reduce the size of myocardial infarct in pigs. The aim of this study was to investigate the ischemia-protective effect of Danegaptide in a porcine renal I/R-I model with two weeks follow up.Unilateral renal I/R-I was induced in pigs by clamping the left renal artery over a two hour period. The model allowed examination of renal blood flow by magnetic resonance imaging (MRI and the measurement of single kidney GFR two weeks after injury. Eleven animals were randomized to Danegaptide-infusion while nine animals received placebo. Kidney histology and urinary neutrophil gelatinase-associated lipocalin (NGAL excretion were included as markers of AKI.Unilateral kidney I/R-I resulted in an immediate ~50% GFR reduction, associated with a four-fold increase in urinary NGAL-excretion. Fourteen days after I/R-I, the total GFR was ~75% of baseline with a significantly lower GFR in the injured left kidney compared to the right kidney. No differences in GFR were observed between the treated and non-treated animals immediately after I/R-I or at Day 14. Furthermore, no differences were observed in the urinary excretion of NGAL, renal blood flow or other markers of renal function.As expected this porcine renal I/R-I model was associated with reduced GFR two weeks after injury. Danegaptide did not improve renal function after I/R-I.

Comparison of the effect of fatty alcohols on the permeation of melatonin between porcine and human skin.

Science.gov (United States)

Andega, S; Kanikkannan, N; Singh, M

2001-11-09

Melatonin (MT) is a hormone secreted by the pineal gland that plays an important role in the regulation of the circadian sleep-wake cycle. It would be advantageous to administer MT using a transdermal delivery system for the treatment of sleep disorders such as delayed sleep syndrome, jet lag in travelers, cosmonauts and shift workers. The porcine skin has been found to have similar morphological and functional characteristics as human skin. The elastic fibres in the dermis, enzyme pattern of the epidermis, epidermal tissue turnover time, keratinous proteins and thickness of epidermis of porcine skin are similar to human skin. However, the fat deposition and vascularisation of the cutaneous glands of porcine skin are different from human skin. In addition, porcine skin has been found to have a close permeability character to human skin. However, the comparative effect of chemical penetration enhancers on the permeation of drugs between porcine and human skin has not been reported. The purpose of this study was to compare the effect of fatty alcohols on the permeability of porcine and human skin using MT as a model compound. The effect of saturated fatty alcohols (octanol, nonanol, decanol, undecanol, lauryl alcohol, tridecanol, myristyl alcohol) and unsaturated fatty alcohols (oleyl alcohol, linoleyl alcohol, linolenyl alcohol) at 5% concentration was tested across dermatomed porcine and human skin. Our studies showed a parabolic relationship between the carbon chain length of saturated fatty alcohols and permeation enhancement of MT with both porcine and human skin. Maximum permeation of MT was observed when fatty alcohol carbon chain length was 10. In general, as the level of unsaturation increased from one to two double bonds, there was an increase in the permeation of MT both in porcine and human skin. However, a decrease in the permeation was observed with three double bonds. Regression analysis using the steady state flux data showed a significant positive

En bid af ældres hverdagsliv

DEFF Research Database (Denmark)

Bundgaard, Karen Marie; Top Christensen, Boye

2000-01-01

Undersøgelsen har udforsket hjemmeboende ældres egen forståelse og oplevelse af måltiddsaktiviteters betydning, og hvilke behov aktiviteterne dækker hos de ældre. Aktiviteterne har betydninger der knytter sig til maden men aktiviteterne har også betydninger i sig selv. Undersøgelsen er en af fler...

Programmering af applikationer med dSPACE real-time værktøjer

DEFF Research Database (Denmark)

Voigt, Kristian

1998-01-01

real-time direkte på et virkeligt system. Reguleringen af systemet sker vha. et DSP- og I/O-kort. En model af systemet opbygges vha. Matlab/Simulink fra firmaet The Mathworks. Modellen oversættes til C-kode vha. Real-Time Workshop fra firmaet The Mathworks. For at gøre C-koden hardwarespecifik bruges...... software fra hardwareleverandøren - dSPACE. Firmaet dSPACE har desuden levereret software til at monitorere real-time værdier i systemet, og software til at ændre på parametre i modellen i real-time.I brugervejledningens Appendix F findes et eksempel, der gennemgår hele forløbet med opstart af programmer...

Sodium nitroprusside enhanced cardiopulmonary resuscitation improves short term survival in a porcine model of ischemic refractory ventricular fibrillation.

Science.gov (United States)

Yannopoulos, Demetris; Bartos, Jason A; George, Stephen A; Sideris, George; Voicu, Sebastian; Oestreich, Brett; Matsuura, Timothy; Shekar, Kadambari; Rees, Jennifer; Aufderheide, Tom P

2017-01-01

Sodium nitroprusside (SNP) enhanced CPR (SNPeCPR) demonstrates increased vital organ blood flow and survival in multiple porcine models. We developed a new, coronary occlusion/ischemia model of prolonged resuscitation, mimicking the majority of out-of-hospital cardiac arrests presenting with shockable rhythms. SNPeCPR will increase short term (4-h) survival compared to standard 2015 Advanced Cardiac Life Support (ACLS) guidelines in an ischemic refractory ventricular fibrillation (VF), prolonged CPR model. Sixteen anesthetized pigs had the ostial left anterior descending artery occluded leading to ischemic VF arrest. VF was untreated for 5min. Basic life support was performed for 10min. At minute 10 (EMS arrival), animals received either SNPeCPR (n=8) or standard ACLS (n=8). Defibrillation (200J) occurred every 3min. CPR continued for a total of 45min, then the balloon was deflated simulating revascularization. CPR continued until return of spontaneous circulation (ROSC) or a total of 60min, if unsuccessful. SNPeCPR animals received 2mg of SNP at minute 10 followed by 1mg every 5min until ROSC. Standard ACLS animals received 0.5mg epinephrine every 5min until ROSC. Primary endpoints were ROSC and 4-h survival. All SNPeCPR animals (8/8) achieved sustained ROSC versus 2/8 standard ACLS animals within one hour of resuscitation (p=0.04). The 4-h survival was significantly improved with SNPeCPR compared to standard ACLS, 7/8 versus 1/8 respectively, p=0.0019. SNPeCPR significantly improved ROSC and 4-h survival compared with standard ACLS CPR in a porcine model of prolonged ischemic, refractory VF cardiac arrest. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Targeted Porcine Genome Engineering with TALENs

DEFF Research Database (Denmark)

Luo, Yonglun; Lin, Lin; Golas, Mariola Monika

2015-01-01

confers precisely editing (e.g., mutations or indels) or insertion of a functional transgenic cassette to user-designed loci. Techniques for targeted genome engineering are growing dramatically and include, e.g., zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs......, including construction of sequence-specific TALENs, delivery of TALENs into primary porcine fibroblasts, and detection of TALEN-mediated cleavage, is described. This chapter is useful for scientists who are inexperienced with TALEN engineering of porcine cells as well as of other large animals....

Sammenligning af laboratorieforsøg med kemiske, biologiske og termiske metoder til oprensning af residual fri fase under grundvandsspejlet i Kærgård Plantage

DEFF Research Database (Denmark)

Christophersen, Mette; Christensen, Jørgen Fjeldsø; Jørgensen, Torben H.

, sulfonamider, barbiturater, anilin, cyanid og kviksølv, og det har resulteret i en meget kompleks forurening. Efter afgravning af 4000 tons meget forurenet jord ned til grundvandsspejlet i to af de seks gruber er næste skridt afprøvning af forskellige metoder til fjernelse af den kraftige grundvandsforurening...

Development of porcine model of chronic tachycardia-induced cardiomyopathy.

Science.gov (United States)

Paslawska, Urszula; Gajek, Jacek; Kiczak, Liliana; Noszczyk-Nowak, Agnieszka; Skrzypczak, Piotr; Bania, Jacek; Tomaszek, Alicja; Zacharski, Maciej; Sambor, Izabela; Dziegiel, Piotr; Zysko, Dorota; Banasiak, Waldemar; Jankowska, Ewa A; Ponikowski, Piotr

2011-11-17

There are few experimental models of heart failure (HF) in large animals, despite structural and functional similarities to human myocardium. We have developed a porcine model of chronic tachycardia-induced cardiomyopathy. Homogenous siblings of White Large breed swine (n=6) underwent continuous right ventricular (RV) pacing at 170 bpm; 2 subjects served as controls. In the course of RV pacing, animals developed a clinical picture of HF and were presented for euthanasia at subsequent stages: mild, moderate and end-stage HF. Left ventricle (LV) sections were analyzed histologically and relative ANP, BNP, phospholamban and sarcoplasmic reticulum calcium ATPase 2a transcript levels in LV were quantified by real time RT-PCR. In the course of RV pacing, animals demonstrated reduced exercise capacity (time of running until being dyspnoeic: 6.6 ± 0.5 vs. 2.4 ± 1.4 min), LV dilatation (LVEDD: 4.9 ± 0.4 vs. 6.7 ± 0.4 cm), impaired LV systolic function (LVEF: 69 ± 8 vs. 32 ± 7 %), (all baseline vs. before euthanasia, all p<0.001). LV tissues from animals with moderate and end-stage HF demonstrated local foci of interstitial fibrosis, congestion, cardiomyocyte hypertrophy and atrophy, which was not detected in controls and mild HF animals. The up-regulation of ANP and BNP and a reduction in a ratio of sarcoplasmic reticulum calcium ATPase 2a and phospholamban in failing myocardium were observed as compared to controls. In pigs, chronic RV pacing at relatively low rate can be used as an experimental model of HF, as it results in a gradual deterioration of exercise tolerance accompanied by myocardial remodeling confirmed at subcellular level. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

A novel porcine cell culture based protocol for the propagation of hepatitis E virus

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Walter Chingwaru

2016-08-01

Full Text Available Objective: To present a comprehensive protocol for the processing of hepatitis E virus (HEV infected samples and propagation of the virus in primary cell cultures. Methods: Hepatitis E was extracted from porcine liver and faecal samples following standard protocols. The virus was then allowed to attach in the presence of trypsin to primary cells that included porcine and bovine intestinal epithelial cells and macrophages over a period of up to 3 h. The virus was propagated by rotational passaging through the cell cultures. Propagation was confirmed by immunoblotting. Results: We developed a comprehensive protocol to propagate HEV in porcine cell model that includes (i rotational culturing of the virus between porcine cell types, (ii pre-incubation of infected cells for 210 min, (iii use of a semi-complete cell culture medium supplemented with trypsin (0.33 µg/mL and (iv the use of simple immunoblot technique to detect the amplified virus based on the open reading frame 2/3. Conclusions: This protocol opens doors towards systematic analysis of the mechanisms that underlie the pathogenesis of HEV in vitro. Using our protocol, one can complete the propagation process within 6 to 9 d.

Æstetisk iscenesættelsen af det postindustrielle bylandskab

DEFF Research Database (Denmark)

Kiib, Hans

2013-01-01

det naturskønne, det ruinøse og de pittoreske billede af byen og landskabet spiller en vigtig rolle. Men samtidig iscenesætter den eleverede parkbro byen på en sublim måde forstået som opbyggelse af spændingsfelter mellem den nære og kendte og det fremmede og farlige i en af verdens spektakulære...... kortlægning af parkbroens rolle i bydelens dekonstruktion og nyiscenesættelse i en fremadskuende montage. Det omfatter en æstetisk modstilling af arkitektoniske virkemidler for det skønne, det pittoreske og det sublime og tilsvarende en tregrenet oplevelsespallete hos den besøgende: Den konplemtative væren i...

Ny styring af lærernes autonomi?

DEFF Research Database (Denmark)

Holm, Claus

2015-01-01

Kapitlet ”Ny styring af lærernes autonom?” i bogen Folkeskolen - efter reformen fokuserer på, hvordan en realisering af den vedtagne skolereform og forventningen om en kommende brug af nye forenklede Fælles Mål ud fra en målstyringstænkning ændrer lærernes autonomi til at udøve dømmekraft i...... klasseværelset. Dermed er kapitlets tese, at man ikke – i hvert fald ikke kun – kan tale om et tab af autonomi, men en ændret opfattelse og indhold heraf. Opgaven er derfor at indkredse, hvordan danske læreres autonomi til at udøve professionel dømmekraft forandrer sig, hvis man realiserer idéen om en...... læringsmålstyret undervisning, konkret i form af forenklede Fælles Mål. Og netop spørgsmålet om lærerens autonomi til at udøve professionel dømmekraft bliver i sammenhæng med styring heraf et centralt emne og problematik at afklare. Ikke kun i dansk sammenhæng, men også internationalt....

Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

Energy Technology Data Exchange (ETDEWEB)

Cong, Yingying; Li, Xiaoxue; Bai, Yunyun [College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 (China); Lv, Xiaonan [College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 (China); CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience & Technology of China, Beijing 100090 (China); Herrler, Georg [Institute for Virology, University of Veterinary Medicine, Hannover D-30559 (Germany); Enjuanes, Luis [Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Cantoblanco, Madrid (Spain); Zhou, Xingdong [College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 (China); Qu, Bo [Faculty of Life Sciences, Northeast Agricultural University, Harbin 150030 (China); Meng, Fandan [Institute for Virology, University of Veterinary Medicine, Hannover D-30559 (Germany); Cong, Chengcheng [College Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110161 (China); Ren, Xiaofeng; Li, Guangxing [College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030 (China)

2015-04-15

Infection of polarized intestinal epithelial cells by porcine epidemic diarrhea virus (PEDV) was characterized. Indirect immunofluorescence assay, real-time PCR, and transmission electron microscopy confirmed PEDV can be successfully propagated in immortalized swine small intestine epithelial cells (IECs). Infection involved porcine aminpeptidase N (pAPN), a reported cellular receptor for PEDV, transient expression of pAPN and siRNA targeted pAPN increased and decreased the infectivity of PEDV in IECs, respectively. Subsequently, polarized entry into and release from both Vero E6 and IECs was analyzed. PEDV entry into polarized cells and pAPN grown on membrane inserts occurs via apical membrane. The progeny virus released into the medium was also quantified which demonstrated that PEDV is preferentially released from the apical membrane. Collectively, our data demonstrate that pAPN, the cellular receptor for PEDV, mediates polarized PEDV infection. These results imply the possibility that PEDV infection may proceed by lateral spread of virus in intestinal epithelial cells. - Highlights: • PEDV infection of polarized intestinal epithelial cells (IECs) was characterized. • Porcine aminpeptidase N (pAPN) facilitated PEDV infection in IECs. • PEDV entry into and release from polarized cell via its apical membrane. • PEDV infection may proceed by lateral spread of virus in IECs.

Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

International Nuclear Information System (INIS)

Cong, Yingying; Li, Xiaoxue; Bai, Yunyun; Lv, Xiaonan; Herrler, Georg; Enjuanes, Luis; Zhou, Xingdong; Qu, Bo; Meng, Fandan; Cong, Chengcheng; Ren, Xiaofeng; Li, Guangxing

2015-01-01

Infection of polarized intestinal epithelial cells by porcine epidemic diarrhea virus (PEDV) was characterized. Indirect immunofluorescence assay, real-time PCR, and transmission electron microscopy confirmed PEDV can be successfully propagated in immortalized swine small intestine epithelial cells (IECs). Infection involved porcine aminpeptidase N (pAPN), a reported cellular receptor for PEDV, transient expression of pAPN and siRNA targeted pAPN increased and decreased the infectivity of PEDV in IECs, respectively. Subsequently, polarized entry into and release from both Vero E6 and IECs was analyzed. PEDV entry into polarized cells and pAPN grown on membrane inserts occurs via apical membrane. The progeny virus released into the medium was also quantified which demonstrated that PEDV is preferentially released from the apical membrane. Collectively, our data demonstrate that pAPN, the cellular receptor for PEDV, mediates polarized PEDV infection. These results imply the possibility that PEDV infection may proceed by lateral spread of virus in intestinal epithelial cells. - Highlights: • PEDV infection of polarized intestinal epithelial cells (IECs) was characterized. • Porcine aminpeptidase N (pAPN) facilitated PEDV infection in IECs. • PEDV entry into and release from polarized cell via its apical membrane. • PEDV infection may proceed by lateral spread of virus in IECs

Lokal integration af førtidspensionister

DEFF Research Database (Denmark)

Jacobsen, Joannes; Lindstrøm, Maia

Denne rapport kortlægger livsvilkårene for en bred gruppe af førtidspensionister med fokus på deres hverdag og de vilkår, som omgiver dem. Derudover identificerer rapporten en gruppe af førtidspensionister, der selv ønsker mere social interaktion og indhold i hverdagen. Undersøgelsen viser, at de...

Arbejdsmiljø ved udbud af busdrift

DEFF Research Database (Denmark)

Wiegmann, Inger-Marie; Mathiesen, Karin; Hasle, Peter

En undersøgelse af sammenhængen mellem arbejdsmiljø og udbud af busdrift i tre trafikselskabet. Resultaterne peger på at arbejdsmiljøet går fra udelukkende til at være et anliggende mellem arbejdsgiver og arbejdstager til et trepartsforhold hvor også udbyder (trafikselskabet) får et ansvar og har...

Udvikling og afprøvning af it-støttet vejledning af lærerstuderende i praktik

DEFF Research Database (Denmark)

Clemens, Heidie

2011-01-01

samarbejdet mellem læreruddannelsen og praktikinstitutionerne omkring vejledning af de studerende i praktikken via Skype eller VIAs platform og at afprøve en vejledningsform, der har karakter af procesvejledning. Erfaringer med at afprøve den virtuelle mødeform i denne sammenhæng muliggør mødet mellem...

Circovirose suína Porcine circovirosis: a review

Directory of Open Access Journals (Sweden)

Ticiana do Nascimento França

2005-06-01

Full Text Available Por meio de revisão da literatura pertinente foram coligidos e são apresentados os principais dados relativos aos aspectos epidemiológicos, clínicos, anátomo e histopatológicos observados na infecção por Circovírus Porcino tipo 2 (PCV-2 em suínos. São abordados a Síndrome Definhante Multissistêmica dos Suínos Desmamados (SDMDS, o Tremor Congênito Suíno (TCS, a Síndrome da Nefropatia e Dermatite Porcina (SNDP, bem como outras enfermidades associadas ou correlatas, a Síndrome Respiratória e Reprodutiva Porcina (SRRP, a Pneumonia Necrotizante Proliferativa (PNP e as falhas reprodutivas. Uma vez que a SDMSD já foi registrada na Região Sul do Brasil e no Estado do Rio de Janeiro esse estudo objetiva chamar a atenção para o especial significado dessa virose para a suinocultura brasileira, em função dos prejuízos econômicos por ela determinados.The literature of Porcine Circovirosis, including the main data on epidemiology and clinical, macroscopic and microscopic alterations of the infection of swine by Porcine Circovirus type 2 (PCV-2, is reviewed. There are various forms of infection: the [Porcine] Postweaning Multisystemic Wasting Syndrome (PMWS, Porcine Congenital Tremor, Porcine Dermatitis and Nephropathy Syndrome, and other associated or correlated diseases as the Porcine Reproductive and Respiratory Syndrome, Proliferative Necrotizing Pneumonia, and reproductive disorders. As PMWS already has been reported from southern Brazil and from the state of Rio de Janeiro, the objective of this review is to draw attention to the implications of this virosis for swine production in Brazil and its economical importance.

Telefonitis: unge danskeres brug af telefonen i IT-tidsalderen

Directory of Open Access Journals (Sweden)

Gitte B. Stald

2000-09-01

Full Text Available Den udbredte brug af mobiltelefoner har sat fornyet fokus på telefonens betydning i vores tid. Som følge af den informationsteknologiske udvikling ændrer telefonen karakter fra at være en kanal for kommunikation til at være et medie for flere funktioner, samtidig med at dens konkrete og sym- bolske betydning forstærkes. Dette kan i særlig grad aflæses i større børns og unges brug af telefonen, der dels er karakteriseret ved stor selvfølgelig- hed, dels ved at have en betydning, der rækker ud over funktioner: den er markør af ungdomskultur, den er garant for tryghed, og den muliggør fast- holdelse af sociale og personlige relationer. Telefonen befordrer etablering- en af et virtuelt rum, hvor intimitet og fortrolighed er mulig. Set i lyset af den informationsteknologiske udvikling, der har bragt e-mail, chat og andre for- mer for on- og offline kommunikation ind i de fleste unge danskeres tilvæ- relse, er det interessant, at telefonen har fastholdt og endog forstærket sin betydning.

Mechanism of porcine liver xanthine oxidoreductase mediated N-oxide reduction of cyadox as revealed by docking and mutagenesis studies.

Directory of Open Access Journals (Sweden)

Chigang Chen

Full Text Available Xanthine oxidoreductase (XOR is a cytoplasmic molybdenum-containing oxidoreductase, catalyzing both endogenous purines and exogenous compounds. It is suggested that XOR in porcine hepatocytes catalyzes the N-oxide reduction of quinoxaline 1,4-di-N-oxides (QdNOs. To elucidate the molecular mechanism underlying this metabolism, the cDNA of porcine XOR was cloned and heterologously expressed in Spodoptera frugiperda insect cells. The bovine XOR, showing sequence identity of 91% to porcine XOR, was employed as template for homology modeling. By docking cyadox, a representative compound of QdNOs, into porcine XOR model, eight amino acid residues, Gly47, Asn352, Ser360, Arg427, Asp430, Asp431, Ser1227 and Lys1230, were located at distances of less than 4Å to cyadox. Site-directed mutagenesis was performed to analyze their catalytic functions. Compared with wild type porcine XOR, G47A, S360P, D431A, S1227A, and K1230A displayed altered kinetic parameters in cyadox reduction, similarly to that in xanthine oxidation, indicating these mutations influenced electron-donating process of xanthine before subsequent electron transfer to cyadox to fulfill the N-oxide reduction. Differently, R427E and D430H, both located in the 424-434 loop, exhibited a much lower K(m and a decreased V(max respectively in cyadox reduction. Arg427 may be related to the substrate binding of porcine XOR to cyadox, and Asp430 is suggested to be involved in the transfer of electron to cyadox. This study initially reveals the possible catalytic mechanism of porcine XOR in cyadox metabolism, providing with novel insights into the structure-function relationship of XOR in the reduction of exogenous di-N-oxides.

Anmeldelse af: Afghantsy. The Russians in Afghanistan 1979-89

DEFF Research Database (Denmark)

Vestenskov, David

2012-01-01

Baseret på nyligt afklassificeret materiale fra arkiverne i Moskva samt fremstillinger af russiske historikere, tegnes et anderledes og mere nuanceret billede af de sovjetiske erfaringer fra Afghanistan. De første spadestik til opgøret med den amerikanske stempling af den sovjetiske ageren i...

Antitrombotisk behanding af patienter med valvulær atrieflimren

DEFF Research Database (Denmark)

Bovin, Ann; Christensen, Thomas Decker; Grove, Erik Lerkevang

2017-01-01

with valvular AF. Although the definition of valvular AF is disputed, the lack of evidence regarding the use of NOACs for these patients is not. We discuss the definition of valvular AF, the risk of thromboembolism, and the antithrombotic treatment for stroke prevention in these patients....

Combined in vivo and ex vivo analysis of mesh mechanics in a porcine hernia model.

Science.gov (United States)

Kahan, Lindsey G; Lake, Spencer P; McAllister, Jared M; Tan, Wen Hui; Yu, Jennifer; Thompson, Dominic; Brunt, L Michael; Blatnik, Jeffrey A

2018-02-01

Hernia meshes exhibit variability in mechanical properties, and their mechanical match to tissue has not been comprehensively studied. We used an innovative imaging model of in vivo strain tracking and ex vivo mechanical analysis to assess effects of mesh properties on repaired abdominal walls in a porcine model. We hypothesized that meshes with dissimilar mechanical properties compared to native tissue would alter abdominal wall mechanics more than better-matched meshes. Seven mini-pigs underwent ventral hernia creation and subsequent open repair with one of two heavyweight polypropylene meshes. Following mesh implantation with attached radio-opaque beads, fluoroscopic images were taken at insufflation pressures from 5 to 30 mmHg on postoperative days 0, 7, and 28. At 28 days, animals were euthanized and ex vivo mechanical testing performed on full-thickness samples across repaired abdominal walls. Testing was conducted on 13 mini-pig controls, and on meshes separately. Stiffness and anisotropy (the ratio of stiffness in the transverse versus craniocaudal directions) were assessed. 3D reconstructions of repaired abdominal walls showed stretch patterns. As pressure increased, both meshes expanded, with no differences between groups. Over time, meshes contracted 17.65% (Mesh A) and 0.12% (Mesh B; p = 0.06). Mesh mechanics showed that Mesh A deviated from anisotropic native tissue more than Mesh B. Compared to native tissue, Mesh A was stiffer both transversely and craniocaudally. Explanted repaired abdominal walls of both treatment groups were stiffer than native tissue. Repaired tissue became less anisotropic over time, as mesh properties prevailed over native abdominal wall properties. This technique assessed 3D stretch at the mesh level in vivo in a porcine model. While the abdominal wall expanded, mesh-ingrown areas contracted, potentially indicating stresses at mesh edges. Ex vivo mechanics demonstrate that repaired tissue adopts mesh properties, suggesting

Flexicurity - afklaring af et begreb i bevægelse

DEFF Research Database (Denmark)

Larsen, Flemming; Madsen, Per Kongshøj; Bredgaard, Thomas

2007-01-01

Flexicurity er et begreb på vandring. Det blev opfundet i Holland i midten af 1990erne, og er siden blevet en samlebetegnelse for det danske arbejdsmarkedssystem. Aktuelt er opnåelse af flexicurity blevet til topprioriteten for EU-kommissionens forsøg på reformer af arbejdsmarkedsområdet. Dermed...... bliver det et begreb som alle medlemslande er tvunget til at forholde sig til. Flexicurity er med andre ord en retorisk succes. I denne artikel søger vi at dokumentere begrebets sejrsvandring. Den politiske og akademiske omfavnelse af flexicurity indebærer imidlertid en risiko for at begrebet udvandes......, og bliver meningsløst. Vi diskuterer derfor forskellige definitioner af flexicurity for at opnå en større klarhed over, hvad der bør og kan forstås ved flexicurity, og giver til slut et bud på en ny forståelse af flexicurity som analytisk begreb....

Immobilization of pseudorabies virus in porcine tracheal respiratory mucus revealed by single particle tracking.

Directory of Open Access Journals (Sweden)

Xiaoyun Yang

Full Text Available Pseudorabies virus (PRV initially replicates in the porcine upper respiratory tract. It easily invades the mucosae and submucosae for subsequent spread throughout the body via blood vessels and nervous system. In this context, PRV developed ingenious processes to overcome different barriers such as epithelial cells and the basement membrane. Another important but often overlooked barrier is the substantial mucus layer which coats the mucosae. However, little is known about how PRV particles interact with porcine respiratory mucus. We therefore measured the barrier properties of porcine tracheal respiratory mucus, and investigated the mobility of nanoparticles including PRV in this mucus. We developed an in vitro model utilizing single particle tracking microscopy. Firstly, the mucus pore size was evaluated with polyethylene glycol coupled (PEGylated nanoparticles and atomic force microscope. Secondly, the mobility of PRV in porcine tracheal respiratory mucus was examined and compared with that of negative, positive and PEGylated nanoparticles. The pore size of porcine tracheal respiratory mucus ranged from 80 to 1500 nm, with an average diameter of 455±240 nm. PRV (zeta potential: -31.8±1.5 mV experienced a severe obstruction in porcine tracheal respiratory mucus, diffusing 59-fold more slowly than in water. Similarly, the highly negatively (-49.8±0.6 mV and positively (36.7±1.1 mV charged nanoparticles were significantly trapped. In contrast, the nearly neutral, hydrophilic PEGylated nanoparticles (-9.6±0.8 mV diffused rapidly, with the majority of particles moving 50-fold faster than PRV. The mobility of the particles measured was found to be related but not correlated to their surface charge. Furthermore, PEGylated PRV (-13.8±0.9 mV was observed to diffuse 13-fold faster than native PRV. These findings clearly show that the mobility of PRV was significantly hindered in porcine tracheal respiratory mucus, and that the obstruction of PRV

Selective Retrograde Venous Revascularization of the Myocardium when PCI or CABG Is Impossible: Investigation in a Porcine Model

DEFF Research Database (Denmark)

Møller, Christian H; Nørgaard, Martin A; Gøtze, Jens P

2008-01-01

We investigated the possibility of nourishing the myocardium through selective retrograde coronary venous bypass grafting (CVBG) with an off-pump technique and evaluated various methods of monitoring the physiological effects of this procedure. In a porcine model, the left internal mammary artery...... tension decreased, but with time some recovery was seen. Cardiac troponin T was elevated. Histological analysis showed ischemic changes. In control pigs, microdialysis was performed for 1.5 hours up to LAD artery ligation, after which all pigs died in ventricular fibrillation arrest. No increase...

Randomiseret klinisk undersøgelse af effekten af kognitiv adaptationstræning: design og metodediskussion

DEFF Research Database (Denmark)

Hansen, Jens Peter; Østergaard, Birte; Nordentoft, Merete

2009-01-01

  Kognitiv adaptations træning er afprøvet gennem randomiserede studier i udlandet, men er ikke afprøvet på nydebuterede patienter, som modtager intensiv pleje og behandling. I denne artikel præsenteres et forskningsprojekt, som skal afgøre om kognitiv adaptations træning er velegnet i en dansk...... kontekst som supplement til nuværende tilbud til patienter med debuterende skizofreni. Studiet gennemføres som et randomiseret multicenter forsøg, hvor 164 konsekutivt inkluderede patienter fordeles til henholdsvis kognitiv adaptations træning og standardbehandling. Effekten af interventionen evalueres med......, fordelingsprocedure, bortfald, udvælgelse af patienter og sammenlignelighed mellem tilknyttede centre. Dette studie er det første studie, hvor kognitiv adaptations træning sammenlignes med et ambulant tilbud bestående af psykiatrisk behandling kombineret med hyppige hjemmebesøg ved distriktssygeplejersker. Studiet...

Pædagogers formidling af naturen i naturbørnehaver

DEFF Research Database (Denmark)

Ejbye-Ernst, Niels

2012-01-01

, interviews, andenordensrefleksioner og udsagn fra 500 pædagoger) giver mulighed for triangulering af resultaterne (Denzin & Lincoln 1998) og dermed mulighed for at belyse området alsidigt. I forskningsprojektets sigte indgår både en analyse af feltet gennem gennemgribende iagttagelser af pædagogers...

Modellering af kviksølvs globale spredning - 1. rejsebrev fra Harvard

DEFF Research Database (Denmark)

Sørensen, Anne Lærke; Skov, Henrik; Glasius, Marianne

2008-01-01

Spredning af kviksølv via atmosfæren er et globalt problem. Kviksølv har en lang levetid i atmosfæren og deponerer derfor langt fra den oprindelige kilde. Herefter kan det bioakkumulere, hvilket især sker i den akvatiske fødekæde (figur 1). Gennem bioakkumulationen ender kviksølv i det øverste le...

Endoscopic intestinal bypass creation by using self-assembling magnets in a porcine model.

Science.gov (United States)

Ryou, Marvin; Agoston, A Tony; Thompson, Christopher C

2016-04-01

A purely endoluminal method of GI bypass would be desirable for the treatment of obstruction, obesity, or metabolic syndrome. We have developed a technology based on miniature self-assembling magnets that create large-caliber anastomoses (Incisionless Anastomosis System [IAS]). The aim of this study was to evaluate procedural characteristics of IAS deployment and long-term anastomotic integrity and patency. We performed a 3-month survival study of Yorkshire pigs (5 interventions, 3 controls). Intervention pigs underwent simultaneous enteroscopy/colonoscopy performed with the animals under intravenous sedation. The IAS magnets were deployed and coupled with reciprocal magnets under fluoroscopy. Every 3 to 6 days pigs underwent endoscopy until jejunocolonic anastomosis (dual-path bypass) creation and magnet expulsion. Necropsies and histological evaluation were performed. The primary endpoints were technical success; secondary endpoints of anastomosis integrity, patency, and histological characteristics were weight trends. Under intravenous sedation, endoscopic bypass creation by using IAS magnets was successfully performed in 5 of 5 pigs (100%). Given porcine anatomy, the easiest dual-path bypass to create was between the proximal jejunum and colon. The mean procedure time was 14.7 minutes. Patent, leak-free anastomoses formed by day 4. All IAS magnets were expelled by day 12. All anastomoses were fully patent at 3 months with a mean diameter of 3.5 cm. The mean 3-month weight was 45 kg in bypass pigs and 78 kg in controls (P = .01). At necropsy, adhesions were absent. Histology showed full re-epithelialization across the anastomosis without fibrosis or inflammation. Large-caliber, leak-free, foreign body-free endoscopic intestinal bypass by using IAS magnets can be safely and rapidly performed in the porcine by model using only intravenous sedation. Copyright © 2016 American Society for Gastrointestinal Endoscopy. Published by Elsevier Inc. All rights reserved.

Retsbeskyttelsen af fostre og befrugtede æg

DEFF Research Database (Denmark)

Herrmann, Janne Rothmar

tydeliggøres og analyseres forskellige problemstillinger: -Abort og fosterreduktion - Fosterdiagnostik - Fosteret som patient og forsøgsperson - Anvendelse af føtale celler og væv - Fosterets status som afdød - Fertilitetsbehandling og sortering af befrugtede æg - Fertilitetsturisme - Stamcelleforskning...

Selskaberne er en del af staten

DEFF Research Database (Denmark)

Hein Jessen, Mathias

2016-01-01

Lækket af Panama Papers fremhæver endnu engang den paradoksale rolle, ikke mindst multinationale selskaber spiller i politik og økonomi: De er både skabt af staten, og fremstår stadig som uden for og i opposition til staten. Hvis vi vil gøre noget ved problemet, må vi forstå, hvordan stat og...

Omrids af en trækultur

DEFF Research Database (Denmark)

Rauff Greisen, Johannes

2007-01-01

høje prioritering af de fysiske rammer kan aflæses i de store linjer såvel som i de redelige detaljer. Artiklens spørgsmål er, hvorledes håndværkstraditionens kvaliteter og kerne kan overleveres til det moderne byggeri, der også i Vorarlberg i stigende grad præges af industrialiseringen, samt i hvilke...

Experimental porcine cysticercosis using infected beetles with Taenia solium eggs.

Science.gov (United States)

Gomez-Puerta, Luis A; Garcia, Hector H; Gonzalez, Armando E

2018-07-01

Beetles are intermediate hosts for human and animal parasites, and several beetle species have been shown to carry Taenia eggs. An experimental porcine cysticercosis infection model was developed using beetles (Ammophorus rubripes) infected with Taenia solium eggs and then using these beetles for oral pig challenge. A total of 18 three months-old Landrace pigs were divided in four groups. Pigs from groups 1, 2, and 3 (n = 6 pigs per group) were challenged with one, three, and six beetles infected with T. solium eggs, containing approximately 52, 156 or 312 eggs respectively. Pigs were necropsied 12 weeks after infection to assess the presence of T. solium metacestode. Porcine cysticercosis by T. solium was produced in 17 out of 18 pigs (94.4%) challenged with infected beetles, all infected pigs had viable cysts. Only one pig from group 1 was negative to the presence of cysts. The median number of metacestodes per pig in groups 1, 2, and 3 were 2 (range 0-71), 26 (range 5-33) and 40 cysts (range 4-111), respectively. Experimental porcine cysticercosis infection is consistently obtained using beetles as mechanical vectors for T. solium eggs. Copyright © 2018 Elsevier B.V. All rights reserved.

The kinetics of interaction of porcine - alpha-, and porcine - beta -trypsin with intact and modified soybean trypsin inhibitor (kunitz)

International Nuclear Information System (INIS)

Hamid, M.A.

1994-01-01

The association of porcine trypsin with soybean trypsin inhibitor (Kunitz) resulted in characteristic changes in absorption spectrum, indicating an alteration of the micro environments of the enzyme chromophores as a consequence of the interaction. The rates of formation of the stable trypsin - inhibitor complexes from porcine - alpha - trypsin and soybean trypsin inhibitor and from porcine - beta - trypsin and either intact or modified soybean trypsin inhibitor were measured by mixing the equimolar concentration of the reactants in a Stopped - Flow apparatus at pH (4.5 to 10.0). The reaction of trypsin with soybean trypsin inhibitor was of first order with respect to the concentration of the reactants used. The rates of dissociation of the stable complexes, alpha - trypsin - soybean trypsin inhibitor, beta -trypsin - soybean trypsin inhibitor and beta -trypsin modified soybean trypsin inhibitor were also measured at pH (1.92 to 3.58). The values of first order rate constant, k/sub D/ obtained for the dissociation of all the three complexes were identical with one another. The kinetics results obtained for the porcine trypsin were compared with those of bovine trypsin system and it was suggested that the reaction mechanisms in both these systems were identical. (author)

AF Sites

Science.gov (United States)

AF Bands Air Force Band Recordings Air National Guard Band of the Midwest Air National Guard Band of U.S. Air Force Bands U.S. Air Force Heartland of America Band U.S. Air Force Heritage of America Band U.S. Air Forces in Europe Band US Air Forces Central Command Band Medical 42d Medical Group 59th

Arbejdsformer under anvendelse af 4. generationsværktøjer

DEFF Research Database (Denmark)

Christensen, Søren; Grønbæk, Kaj; Rolskov, Tove

En belysning af sammenhængen mellem arbejdsformer og anvendelse af 4. generationsværktøjer i systemudvikling -- baseret på en undersøgelse af 9 systemudviklingsprojekter og et litteraturstudium...

New radiofrequency device to reduce bleeding after core needle biopsy: Experimental study in a porcine liver model

International Nuclear Information System (INIS)

Lim, Sang Hyeok; Rhim, Hyun Chul; Lee, Min Woo; Song, Kyoung Doo; Kang, Tae Wook; Kim, Young Sun; Lim, Hyo Keun

2017-01-01

To evaluate the in vivo efficiency of the biopsy tract radiofrequency ablation for hemostasis after core biopsy of the liver in a porcine liver model, including situations with bleeding tendency and a larger (16-gauge) core needle. A preliminary study was performed using one pig to determine optimal ablation parameters. For the main experiment, four pigs were assigned to different groups according to heparinization use and biopsy needle caliber. In each pig, 14 control (without tract ablation) and 14 experimental (tract ablation) ultrasound-guided core biopsies were performed using either an 18- or 16-gauge needle. Post-biopsy bleeding amounts were measured by soaking up the blood for five minutes. The results were compared using the Mann-Whitney U test. The optimal parameters for biopsy tract ablation were determined as a 2-cm active tip electrode set at 40-watt with a tip temperature of 70–80℃. The bleeding amounts in all experimental groups were smaller than those in the controls; however they were significant in the non-heparinized pig biopsied with an 18-gauge needle and in two heparinized pigs (p < 0.001). In the heparinized pigs, the mean blood loss in the experimental group was 3.5% and 13.5% of the controls biopsied with an 18- and 16-gauge needle, respectively. Radiofrequency ablation of hepatic core biopsy tract ablation may reduce post-biopsy bleeding even under bleeding tendency and using a larger core needle, according to the result from in vivo porcine model experiments

New radiofrequency device to reduce bleeding after core needle biopsy: Experimental study in a porcine liver model

Energy Technology Data Exchange (ETDEWEB)

Lim, Sang Hyeok; Rhim, Hyun Chul; Lee, Min Woo; Song, Kyoung Doo; Kang, Tae Wook; Kim, Young Sun; Lim, Hyo Keun [Dept. of Radiology and Center for Imaging Science, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of)

2017-01-15

To evaluate the in vivo efficiency of the biopsy tract radiofrequency ablation for hemostasis after core biopsy of the liver in a porcine liver model, including situations with bleeding tendency and a larger (16-gauge) core needle. A preliminary study was performed using one pig to determine optimal ablation parameters. For the main experiment, four pigs were assigned to different groups according to heparinization use and biopsy needle caliber. In each pig, 14 control (without tract ablation) and 14 experimental (tract ablation) ultrasound-guided core biopsies were performed using either an 18- or 16-gauge needle. Post-biopsy bleeding amounts were measured by soaking up the blood for five minutes. The results were compared using the Mann-Whitney U test. The optimal parameters for biopsy tract ablation were determined as a 2-cm active tip electrode set at 40-watt with a tip temperature of 70–80℃. The bleeding amounts in all experimental groups were smaller than those in the controls; however they were significant in the non-heparinized pig biopsied with an 18-gauge needle and in two heparinized pigs (p < 0.001). In the heparinized pigs, the mean blood loss in the experimental group was 3.5% and 13.5% of the controls biopsied with an 18- and 16-gauge needle, respectively. Radiofrequency ablation of hepatic core biopsy tract ablation may reduce post-biopsy bleeding even under bleeding tendency and using a larger core needle, according to the result from in vivo porcine model experiments.

Logning af teledata i lyset af Tele2-dommen

DEFF Research Database (Denmark)

Lentz, Lene Wacher

2017-01-01

De danske teleudbydere har siden 2007 været underlagt en pligt efter logningsbekendtgørelsen til at registrere data om al telekommunikation for at sikre at data er tilgængelige ved en eventuel senere efterforskning. Logningspligten må nu revideres og i vidt omfang afvikles som følge af EU-domstol...

Opfattelser af integration blandt unge med migrantbaggrund

DEFF Research Database (Denmark)

Skytte, Marianne; Bryderup, Inge

2014-01-01

I artiklen formidles analyser af otte interview med unge med migrantbaggrund. Der er fokus på de unges oplevelser og opfattelser af integration. Der er tale om komplekse processer og vekselvirkninger i forholdet mellem de samfundsmæssige kategoriseringer og individernes selvopfattelser. De unge o...

A retinaculum-sparing surgical approach preserves porcine stifle joint cartilage in an experimental animal model of cartilage repair.

Science.gov (United States)

Bonadio, Marcelo B; Friedman, James M; Sennett, Mackenzie L; Mauck, Robert L; Dodge, George R; Madry, Henning

2017-12-01

This study compares a traditional parapatellar retinaculum-sacrificing arthrotomy to a retinaculum-sparing arthrotomy in a porcine stifle joint as a cartilage repair model. Surgical exposure of the femoral trochlea of ten Yucatan pigs stifle joint was performed using either a traditional medial parapatellar approach with retinaculum incision and luxation of the patella (n = 5) or a minimally invasive (MIS) approach which spared the patellar retinaculum (n = 5). Both classical and MIS approaches provided adequate access to the trochlea, enabling the creation of cartilage defects without difficulties. Four full thickness, 4 mm circular full-thickness cartilage defects were created in each trochlea. There were no intraoperative complications observed in either surgical approach. All pigs were allowed full weight-bearing and full range of motion immediately postoperatively and were euthanized between 2 and 3 weeks. The traditional approach was associated with increased cartilage wear compared to the MIS approach. Two blinded raters performed gross evaluation of the trochlea cartilage surrounding the defects according to the modified ICRS cartilage injury classification. The traditional approach cartilage received a significantly worse score than the MIS approach group from both scorers (3.2 vs 0.8, p = 0.01 and 2.8 vs 0, p = 0.005 respectively). The MIS approach results in less damage to the trochlear cartilage and faster return to load bearing activities. As an arthrotomy approach in the porcine model, MIS is superior to the traditional approach.

Studerendes mulighed for opnåelse af kompetence til udvikling af professionen i positionen mellem central styring og decentral autonomi

DEFF Research Database (Denmark)

Dau, Susanne

Dette paper bygger på et casestudie af implementeringen af blended læring i to professionsuddannelser ved University College Nord i Danmark. I paperet afdækkes studerendes udfordringer i positionen mellem central styring og decentral autonomi: 1) På den ene side de ydre styringsbetingelser, og...... kompetencemål skal de studerendes opnå selvstyret læring for at kvalificere sig til kommende profession, videreuddannelse, livslang læring og professionsudvikling. Denne kvalificering kan være i risiko for at blive undergravet af den centrale styring. På baggrund heraf undersøges problemstillingen: Hvordan......, praktikken og de studerendes fremtidige professionspraksis. Det anbefales, at der arbejdes både centralt og decentralt med at minimere de studerendes dilemmaposition mellem central styring og autonomi til gavn for samfundet og brugerne af de professionelles ydelser....

Forekomst af resistente bakterier og forbrug af antibiotika til hunde

DEFF Research Database (Denmark)

Pedersen, Karl; Pedersen, Kristina; Jensen, Helene

2007-01-01

), Pasteurella multocida (n=25), Bordetella bronchiseptica (n=14), Proteus spp. (n=29), og E. coli (n=449). I undersøgelsen anvendtes data fra VetStat databasen. Størstedelen af de antibiotika, der bruges til hunde er bredspektrede. Penicilliner med udvidet spektrum, cephalosporiner samt sulphonamider...

Transkateterlukning af venstre atriums aurikel til forebyggelse af apoplexia cerebri

DEFF Research Database (Denmark)

Loupis, Anastasia M; De Backer, Ole; Ihlemann, Nikolaj

2014-01-01

In patients with atrial fibrillation (AF) and increased risk of stroke, oral anticoagulation (OAC) is the standard treatment for stroke prevention - however, this therapy also carries a high risk of bleeding. Percutaneous closure of the left atrial appendage (LAA) has been suggested to be an alte...

Bacteriospermia in extended porcine semen.

Science.gov (United States)

Althouse, Gary C; Lu, Kristina G

2005-01-15

Bacteriospermia is a frequent finding in freshly extended porcine semen and can result in detrimental effects on semen quality and longevity if left uncontrolled. The primary source of bacterial contamination is the boar. Other sources that have been identified include environment, personnel, and the water used for extender preparation. A 1-year retrospective study was performed on submissions of extended porcine semen for routine quality control bacteriological screening at the University of Pennsylvania. Out of 250 sample submissions, 78 (31.2%) tested positive for bacterial contamination. The most popular contaminants included Enterococcus spp. (20.5%), Stenotrophomonas maltophilia (15.4%), Alcaligenes xylosoxidans (10.3%), Serratia marcescens (10.3%), Acinetobacter lwoffi (7.7%), Escherichia coli (6.4%), Pseudomonas spp. (6.4%), and others (23.0%). Prudent individual hygiene, good overall sanitation, and regular monitoring can contribute greatly in controlling bacterial load. Strategies that incorporate temperature-dependent bacterial growth and hyperthermic augmentation of antimicrobial activity are valuable for effective control of susceptible bacterial loads. Aminoglycosides remain the most popular antimicrobial class used in porcine semen extenders, with beta-lactam and lincosamide use increasing. With the advent of more novel antimicrobial selection and semen extender compositions in swine, prudent application and understanding of in vitro pharmacodynamics are becoming paramount to industry success in the use of this breeding modality.

“All That Jazz” - bagsiden af showbizz

DEFF Research Database (Denmark)

Eigtved, Michael

2017-01-01

Artiklen, der er skrevet til programmet ifbm premieren på musicalen Chicago på Det Ny Teater beskriver den tendens i broadwaymusicals, som begynder i slutningen af 1960’erne med bla forestillingen Cabaret, hvor det er skyggesiden af underholdningens industrien, der selvrefleksivt er emnet for for...

Anvendelse af webkonference på Syddansk Universitet

DEFF Research Database (Denmark)

Kjær, Christopher; Christensen, Inger-Marie F.; Blok, Rasmus

2009-01-01

Artiklen videregiver en række praktiske erfaringer med anvendelse af webkonferencesystemet Adobe Connect Pro i forbindelse med undervisning på Syddansk Universitet (SDU). Via tre pilotprojekter fra forskellige fagområder præsenteres de første konkrete og indledende erfaringer med anvendelsen af det...

Ingeniørens rolle ved skabelse af innovative koncepter

DEFF Research Database (Denmark)

McAloone, Tim C.

2007-01-01

sågar også konkurrencer for skolebørn. En af årsagerne til konkurrencerne, er, at mange virksomheder anser konceptudviklingsaktiviteten for værende af stigende vigtighed. Det er interessant at bemærke, når man studerer disse forskellige konkurrencers rammer, regler og mål, at fællesnævneren for alle...... "innovative koncepter"? Først vedrørende en forståelse af "innovation" i den industrielle kontekst. Udover den relativt sikre og simplistiske definition af: innovation = opfindelse + markedsmæssig succes, er der kun ganske få forsøg i den danske industrikontekst på at definere målene for, eller aktiviteterne...... skabt (kerne produktindhold). Indtil videre er der ikke blevet leveret nogle af disse pc?er, så den kan stadig betragtes som et koncept. Men man har i hvert fald gjort sit bedste for at overveje alle teknologiske, sociale og design-aspekter af konceptet. Moralen her er, at vi ingeniører skal skærpe...

A novel porcine model of implant associated osteomyelitis: a comprehensive analysis of local, regional and systemic response

DEFF Research Database (Denmark)

Jensen, Louise Kruse; Koch, Janne; Dich-Jorgensen, Kirstine

2017-01-01

Pigs are favorable experimental animals for infectious diseases in humans. However, implant associated osteomyelitis (IAO) models in pigs have only been evaluated using high-inoculum infection (>108 CFU) models in 1975 and 1993. Therefore, the aim of this paper was to present a new low inoculum...... porcine model of human IAO based on 42 experimental pigs. The model was created by drilling an implant cavity in the tibial bone followed by insertion of a small steel implant and simultaneous inoculation of Staphylococcus aureus bacteria (n = 32) or saline (n = 10). The infected pigs were either...... inoculated with 104 CFU (n = 26) or 102 and 103 CFU (n = 6). All animals were euthanized five days after insertion of implants. Pigs receiving the high-inoculum infections showed a significantly higher volume of bone lesion, number of neutrophils around the implant, concentrations of acute phase proteins...

Porcine UCHL1: genomic organization, chromosome localization and expression analysis

DEFF Research Database (Denmark)

Larsen, Knud; Madsen, Lone Bruhn; Bendixen, Christian

2012-01-01

to and protection from Parkinson’s disease. Here we report cloning, characterization, expression analysis and mapping of porcine UCHL1. The UCHL1 cDNA was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The porcine cDNA codes...... in developing porcine embryos. UCHL1 transcript was detected as early as 40 days of gestation. A significant decrease in UCHL1 transcript was detected in basal ganglia from day 60 to day 115 of gestation...

Lukning og indskrænkninger af virksomheder

DEFF Research Database (Denmark)

Pico Geerdsen, Peter; Høgelund, Jan; Larsen, Mona

Der tales meget om de negative konsekvenser af globalisering. I denne rapport søger forfatterne at vurdere risikoen for svagere tilknytning til arbejdsmarkedet og forringet indkomstudvikling blandt fratrådte lønmodtagere i de dele af industrien, der er særligt udsat for konkurrence fra...

Digitalisering af undervisning

DEFF Research Database (Denmark)

Christiansen, René B.; Hansen, Søren; Rossen, Svend

2010-01-01

Rapporten er et bestillingsarbejde fra FU (Forskning og Udvikling) og viser en række eksempler på hvorledes undervisningsforløb kan konstrueres online. Herunder ved brug af både LMS-systemer og web 2.0-værktøjer...

Somatisk embryogense og kryopræservering af eg og bøg

DEFF Research Database (Denmark)

MMH Kristensen, Michel; Find, Jens; Krogstrup, Peter

Somatisk embryogenese og kryopræservering af eg og bøg Traditionel vegetativ formering af eg og bøg er vanskeligt, dyrt og har ingen større praktisk anvendelse i skovbruget. Opbevaring af agern og bog i frøbanker erligeledesproblematiskidetspiringsevnenfalderdramatiskalleredeiløbetafdetoførsteåre......Somatisk embryogenese og kryopræservering af eg og bøg Traditionel vegetativ formering af eg og bøg er vanskeligt, dyrt og har ingen større praktisk anvendelse i skovbruget. Opbevaring af agern og bog i frøbanker...... holdbarheden er flere hundrede år. Arbejdet med eg og bøg er en del af et større projekt, Videreudvikling af bioteknologiske metoder, med henblik på forædling og opbevaring af plantegenetiske ressourcer for skovtræer i Danmark 2003- 2006. Det overordnede formål med projektet var at udvikle metoder til...

Robotic kidney autotransplantation in a porcine model: a procedure-specific training platform for the simulation of robotic intracorporeal vascular anastomosis.

Science.gov (United States)

Tiong, Ho Yee; Goh, Benjamin Yen Seow; Chiong, Edmund; Tan, Lincoln Guan Lim; Vathsala, Anatharaman

2018-03-31

Robotic-assisted kidney transplantation (RKT) with the Da Vinci (Intuitive, USA) platform has been recently developed to improve outcomes by decreasing surgical site complications and morbidity, especially in obese patients. This potential paradigm shift in the surgical technique of kidney transplantation is performed in only a few centers. For wider adoption of this high stake complex operation, we aimed to develop a procedure-specific simulation platform in a porcine model for the training of robotic intracorporeal vascular anastomosis and evaluating vascular anastomoses patency. This paper describes the requirements and steps developed for the above training purpose. Over a series of four animal ethics' approved experiments, the technique of robotic-assisted laparoscopic autotransplantation of the kidney was developed in Amsterdam live pigs (60-70 kg). The surgery was based around the vascular anastomosis technique described by Menon et al. This non-survival porcine training model is targeted at transplant surgeons with robotic surgery experience. Under general anesthesia, each pig was placed in lateral decubitus position with the placement of one robotic camera port, two robotic 8 mm ports and one assistant port. Robotic docking over the pig posteriorly was performed. The training platform involved the following procedural steps. First, ipsilateral iliac vessel dissection was performed. Second, robotic-assisted laparoscopic donor nephrectomy was performed with in situ perfusion of the kidney with cold Hartmann's solution prior to complete division of the hilar vessels, ureter and kidney mobilization. Thirdly, the kidney was either kept in situ for orthotopic autotransplantation or mobilized to the pelvis and orientated for the vascular anastomosis, which was performed end to end or end to side after vessel loop clamping of the iliac vessels, respectively, using 6/0 Gore-Tex sutures. Following autotransplantation and release of vessel loops, perfusion of the

A silk peptide fraction restores cognitive function in AF64A-induced Alzheimer disease model rats by increasing expression of choline acetyltransferase gene

Energy Technology Data Exchange (ETDEWEB)

Cha, Yeseul [College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University, Cheongju (Korea, Republic of); Lee, Sang Hoon [Department of Food Science and Technology, Chungbuk National University, Cheongju (Korea, Republic of); Jang, Su Kil [Division of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung (Korea, Republic of); Guo, Haiyu; Ban, Young-Hwan; Park, Dongsun [College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University, Cheongju (Korea, Republic of); Jang, Gwi Yeong; Yeon, Sungho [Department of Food Science and Technology, Chungbuk National University, Cheongju (Korea, Republic of); Lee, Jeong-Yong [Worldway Co., Ltd., Sejong (Korea, Republic of); Choi, Ehn-Kyoung [College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University, Cheongju (Korea, Republic of); Joo, Seong Soo [Division of Marine Molecular Biotechnology, Gangneung-Wonju National University, Gangneung (Korea, Republic of); Jeong, Heon-Sang, E-mail: hsjeong@cbu.ac.kr [Department of Food Science and Technology, Chungbuk National University, Cheongju (Korea, Republic of); Kim, Yun-Bae, E-mail: solar93@cbu.ac.kr [College of Veterinary Medicine, Veterinary Medical Center, Chungbuk National University, Cheongju (Korea, Republic of)

2017-01-01

This study investigated the effects of a silk peptide fraction obtained by incubating silk proteins with Protease N and Neutrase (SP-NN) on cognitive dysfunction of Alzheimer disease model rats. In order to elucidate underlying mechanisms, the effect of SP-NN on the expression of choline acetyltransferase (ChAT) mRNA was assessed in F3.ChAT neural stem cells and Neuro2a neuroblastoma cells; active amino acid sequence was identified using HPLC-MS. The expression of ChAT mRNA in F3.ChAT cells increased by 3.79-fold of the control level by treatment with SP-NN fraction. The active peptide in SP-NN was identified as tyrosine-glycine with 238.1 of molecular weight. Male rats were orally administered with SP-NN (50 or 300 mg/kg) and challenged with a cholinotoxin AF64A. As a result of brain injury and decreased brain acetylcholine level, AF64A induced astrocytic activation, resulting in impairment of learning and memory function. Treatment with SP-NN exerted recovering activities on acetylcholine depletion and brain injury, as well as cognitive deficit induced by AF64A. The results indicate that, in addition to a neuroprotective activity, the SP-NN preparation restores cognitive function of Alzheimer disease model rats by increasing the release of acetylcholine. - Highlights: • Cognition-enhancing effects of SP-NN, a silk peptide preparation, were investigated. • SP-NN enhanced ChAT mRNA expression in F3.ChAT neural stem cells and Neuro-2a neuroblastoma cells. • Active molecule was identified as a dipeptide composed of tyrosine-glycine. • SP-NN reversed cognitive dysfunction elicited by AF64A. • Neuroprotection followed by increased acetylcholine level was achieved with SP-NN.

New stepwise cooling system for short-term porcine islet preservation.

Science.gov (United States)

Ikemoto, Tetsuya; Noguchi, Hirofumi; Fujita, Yasutaka; Takita, Morihito; Shimoda, Masayuki; Sugimoto, Koji; Jackson, Andrew; Naziruddin, Bashoo; Shimada, Mitsuo; Levy, Marlon F; Matsumoto, Shinichi

2010-10-01

Porcine islets are the most suitable for xeno-islet transplantation. However, it is necessary to establish an effective preservation method against its fragility. Recently, we developed a new cooling and preservation (Keep and Fresh [KFC]; FUJIYA Co, Tokushima, Japan) system, which can maintain viability of hepatocyte. In this study, we examined the KFC for porcine islet preservation. Isolated porcine islets were preserved in CMRL 1066 culture media with bovine serum at 37°C, 22°C, and 4°C and KFC for 24, 48, and 72 hours. Islet recovery rate, purity, and viability were evaluated. After 24-hour preservation, the recovery rate was the highest in the KFC, but no significant difference was found. After 48-hour preservation, the recovery rate by the KFC was 73.9% ± 17.3%, which was significantly higher than the other groups (48.7% ± 28.6% at 37°C, P KFC group, purities and viabilities were the highest among the groups after 24-, 48-, and 72-hour preservation. The KFC system significantly improved porcine islet preservation; therefore, the KFC might be useful for porcine islet preservation.

Sparring på evaluering af Destination Trivsel

DEFF Research Database (Denmark)

Schulz, Anette

, konklusion – hvor udsagn fra elever i henholdsvis indskolingen, mellemtrinnet og udskolingen indledningsvist behandles separat og deskriptivt. Efterfølgende er data analyseret med afsæt i en kryds-case-analyse, hvor der på baggrund af den deskriptive analyse koblet til de respektive målgrupper er udledt en...

Tilsætning af uorganisk og organisk kobber til smågrisefoder

DEFF Research Database (Denmark)

Maribo, Hanne; Poulsen, Hanne Damgaard

2011-01-01

Et højt indhold af kobber i foderet udgør en risiko for belastning af miljøet, og en begrænsning i udledningen af kobber er derfor ønskelig. Brug af organisk bundet kobber (kobberlysin) som alternativ til uorganisk kobber (kobbersulfat) i foderblandinger kan muligvis begrænse det totale forbrug af...

En kvalitativ undersøgelse af gravides valg af nakkefoldscanning

DEFF Research Database (Denmark)

Sørensen, Stina Lou; Dahl, Katja; Beck risør, Mette

2007-01-01

valg. Vi ønskede at undersøge informationens rolle i den gravides forståelse og valg af nakkefoldskanning. Materiale og metoder: Seksogtyve gravide blev interviewet efter første svangrekonsultation og igen efter misdannelseskanningen. Undersøgelsesresultaterne i denne artikel stammer primært fra første...

Porcine circovirus type 2 ORF4 protein binds heavy chain ferritin

Indian Academy of Sciences (India)

2015-08-13

Aug 13, 2015 ... protein is a newly identified viral protein of PCV2 and is involved in ... The porcine alveolar macrophages (PAMs) of a healthy, 2- .... firmation by restriction analysis and DNA sequencing, the ... croscope (Model LSM510 META, Zeiss). ..... circovirus-like viruses from pigs with a wasting disease in the.

Analyse af IPCC delrapport 2 - Effekter, klimatilpasning og sårbarhed

DEFF Research Database (Denmark)

Christensen, Jens Hesselbjerg; Arnbjerg-Nielsen, Karsten; Grindsted, Aslak

. Med afsæt i denne delrapport er der udarbejdet en dansk analyse, der nærmere beskriver effekterne af et ændret klima i Danmark suppleret med den seneste danske forskning udført af det danske Center for Regional Changes in the Earth System (CRES). ”Analyse af IPCC delrapport 2 – Effekter...

Udviklingen på afsætningsmarkederne for dansk svinekød

DEFF Research Database (Denmark)

Jeppesen, Lisbeth Fruensgaard; Bredahl, Lone; Fjord, Thomas Ahle

Danmark er verdens niende største producent af svinekød. Ca. 85% af den danske produktion afsættes i udlandet fordelt på ganske få hovedmarkeder. I Europa er de primære aftagere Tyskland, Storbritanien, Frankrig, Italien, mens Japan er det vigtigste marked uden for Europa, efterfulgt af Rusland...

Extraction Socket Preservation Using Porcine-Derived Collagen Membrane Alone or Associated with Porcine-Derived Bone. Clinical Results of Randomized Controlled Study

Directory of Open Access Journals (Sweden)