WorldWideScience

Sample records for aerobe und anaerobe

  1. Integrated anaerobic and aerobic treatment of sewage.

    NARCIS (Netherlands)

    Kaijun Wang,

    1994-01-01

    This thesis describes results of investigations dealing with sequential concept of anaerobic-aerobic treatment of municipal wastewater. The main purposes of the study were 1) to develop a proper anaerobic hydrolytic pretreatment unit, consisting of a Hydrolysis Upflow Sludge Bed (HUSB-) reactor and

  2. Intraspecific variation in aerobic and anaerobic locomotion

    DEFF Research Database (Denmark)

    Svendsen, Jon Christian; Tirsgård, Bjørn; Cordero, Gerardo A.;

    2015-01-01

    Intraspecific variation and trade-off in aerobic and anaerobic traits remain poorly understood in aquatic locomotion. Using gilthead sea bream (Sparus aurata) and Trinidadian guppy (Poecilia reticulata), both axial swimmers, this study tested four hypotheses: (1) gait transition from steady...... to unsteady (i.e., burst-assisted) swimming is associated with anaerobic metabolism evidenced as excess post exercise oxygen consumption (EPOC); (2) variation in swimming performance (critical swimming speed; U crit) correlates with metabolic scope (MS) or anaerobic capacity (i.e., maximum EPOC); (3...

  3. Intraspecific variation in aerobic and anaerobic locomotion

    DEFF Research Database (Denmark)

    Svendsen, Jon Christian; Tirsgård, Bjørn; Cordero, Gerardo A.;

    2015-01-01

    Intraspecific variation and trade-off in aerobic and anaerobic traits remain poorly understood in aquatic locomotion. Using gilthead sea bream (Sparus aurata) and Trinidadian guppy (Poecilia reticulata), both axial swimmers, this study tested four hypotheses: (1) gait transition from steady...

  4. Energetic and metabolic consequences of aerobic and an-aerobic ATP-production.

    NARCIS (Netherlands)

    Schreurs, V.V.A.M.; Aarts, M.J.; IJssennagger, N.; Hermans, J.; Hendriks, W.H.

    2007-01-01

    ATP, the currency of cellular energy metabolism, can be produced during aerobic and an-aerobic oxidation of metabolic substrates. The aerobic oxidation yields CO2 + H2O as metabolic end products while ATP is produced by oxidative phosphorylation in the mitochondria. Carbohydrate, protein and fat pro

  5. Sequential anaerobic-aerobic treatment for domestic wastewater - A review

    NARCIS (Netherlands)

    Kassab, G.; Halalsheh, M.; Klapwijk, A.; Fayyad, M.; Lier, van J.B.

    2010-01-01

    Introduction, consolidation and even standardization of expensive conventional aerobic systems for domestic wastewater treatment imposed significant financial constraints on the expansion of sanitary services including treatment in developing countries. A viable alternative is the sequential anaerob

  6. Quinones in aerobic and anaerobic mitochondria

    NARCIS (Netherlands)

    van der Klei, S.A.

    2009-01-01

    Ubiquinone (UQ), also known as coenzyme Q, is a ubiquitous quinone and is known to have several functions. One of these functions is electron carrier in the mitochondrial electron transport chain of aerobically functioning bacteria and eukaryotes. In contrast to this aerobically functioning quinone,

  7. Anaerobe Tolerance to Oxygen and the Potentials of Anaerobic and Aerobic Cocultures for Wastewater Treatment

    Directory of Open Access Journals (Sweden)

    M.T. Kato

    1997-12-01

    Full Text Available The anaerobic treatment processes are considered to be well-established methods for the elimination of easily biodegradable organic matter from wastewaters. Some difficulties concerning certain wastewaters are related to the possible presence of dissolved oxygen. The common belief is that anaerobes are oxygen intolerant. Therefore, the common practice is to use sequencing anaerobic and aerobic steps in separate tanks. Enhanced treatment by polishing off the residual biodegradable oxygen demand from effluents of anaerobic reactors, or the biodegradation of recalcitrant wastewater pollutants, usually requires sequenced anaerobic and aerobic bacteria activities. However, the combined activity of both bacteria can also be obtained in a single reactor. Previous experiments with either pure or mixed cultures showed that anaerobes can tolerate oxygen to a certain extent. The oxygen toxicity to methanogens in anaerobic sludges was quantified in batch experiments, as well as in anaerobic reactors. The results showed that methanogens have a high tolerance to oxygen. In practice, it was confirmed that dissolved oxygen does not constitute any detrimental effect on reactor treatment performance. This means that the coexistence of anaerobic and aerobic bacteria in one single reactor is feasible and increases the potentials of new applications in wastewater treatment

  8. Electron beam/biological processing of anaerobic and aerobic sludge

    Science.gov (United States)

    Čuba, V.; Pospíšil, M.; Múčka, V.; Jeníček, P.; Dohányos, M.; Zábranská, J.

    2003-01-01

    Besides common chemical and biological methods, the radiation technology is a promising way of sludge treatment. The paper describes possibilities of combined accelerated electrons/biological processing of both anaerobic and aerobic sludge. Besides one-shot experiments, experimental reactors for the simulation of anaerobic processes have been used. Main effort has been aimed to decrease organic compounds concentration and overall volume of solids, to improve some physico-chemical parameters of sludge, to validate hygienisation effects of the ionising radiation, and in the case of anaerobic sludge, to increase the volume of the produced biogas. Positive effects of the electron beam processing have been observed on all previously named parameters.

  9. Aerobic Capacity and Anaerobic Power Levels of the University Students

    Science.gov (United States)

    Taskin, Cengiz

    2016-01-01

    The aim of study was to analyze aerobic capacity and anaerobic power levels of the university students. Total forty university students who is department physical education and department business (age means; 21.15±1.46 years for male and age means; 20.55±1.79 years for female in department physical education), volunteered to participate in this…

  10. COMMERCIAL-SCALE AEROBIC-ANAEROBIC BIOREACTOR LANDFILL OPERATIONS

    Science.gov (United States)

    A sequential aerobic-anaerobic treatment system has been applied at a commercial scale (3,000 ton per day) municipal solid waste landfill in Kentucky, USA since 2001. In this system, the uppermost layer of landfilled waste is aerated and liquid waste including leachate, surface w...

  11. Sequenced anaerobic-aerobic treatment of hemp pulping wastewaters.

    NARCIS (Netherlands)

    Kortekaas, S.

    1998-01-01

    Biological treatment is an indispensable instrument for water management of non-wood pulp mills, either as internal measure to enable progressive closure of water cycles, or as end of pipe treatment. In this thesis, the sequenced anaerobic-aerobic treatment of hemp ( Cannabis sativa L. ) pulping was

  12. Myocardial damage after continuous aerobic and anaerobic exercise in rats

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    Rostika Flora

    2013-12-01

    Full Text Available Background: Regular physical activity is highly recommended in preventive, curative, and rehabilitative programs in order to promote health, especially cardiovascular health. However, physical activity can also cause sudden death. In athletes, sudden death may occur during sport competitions, with myocardial infarction as the most common etiology. It is suspected that continuous training without any rest-day play a role in cardiac muscle damage and sudden death during competition. Our study was aimed to learn about cardiac muscle adaptation on continuous aerobic and anaerobic physical activity without any rest-day. Methods: The specimens in our study were cardiac muscle tissue obtained from rats that had performed aerobic and anaerobic physical activity on treadmill for 1, 3, 7, and 10 days without any rest-day. Blood gas analysis and hematological assessment were used as parameters of systemic adaptation to hypoxia during physical activity. Moreover, histopathology of cardiac muscle tissue was performed as parameter for cardiac muscle damage.Results: The results showed that aerobic and anaerobic physical activity caused a systemic hypoxic condition and triggered adaptation responses. Cardiac muscle damage occurred on the 10th day in both treatment groups, with more severe damage observed in the group with anaerobic physical activity. The tissue protein level in the anaerobic group increased progressively on the 10th day.Conclusion: Physical activity may result in hypoxia and systemic adaptation. Aerobic and anaerobic physical activities performed for 10 days without any rest-day may cause cardiac muscle damage. (Med J Indones. 2013;22:209-14. doi: 10.13181/mji.v22i4.601Keywords: Cardiac muscle, cardiac muscle damage, histopathology, physical activity

  13. Anaerobes unleashed: Aerobic fuel cells of Geobacter sulfurreducens

    Science.gov (United States)

    Nevin, Kelly P.; Zhang, Pei; Franks, Ashley E.; Woodard, Trevor L.; Lovley, Derek R.

    One of the limitations of power generation with microbial fuel cells is that the anode must typically be maintained under anaerobic conditions. When oxygen is present in the anode chamber microorganisms oxidize the fuel with the reduction of oxygen rather than electron transfer to the anode. A system in which fuel is provided from within a graphite anode and diffuses out to the outer surface of the anode was designed to overcome these limitations. A biofilm of Geobacter sulfurreducens strain KN400, pregrown on the surface of a graphite electrode in a traditional two-chambered system with an anaerobic anode chamber and acetate as an external fuel source, produced current just as well under aerobic conditions when acetate was provided via diffusion from an internal concentrated acetate solution. No acetate was detectable in the external medium. In contrast, aerobic systems in which acetate was provided in the external medium completely failed within 48 h. Internally fed anodes colonized by a strain of KN400 adapted to grow at marine salinities produced current in aerobic seawater as well as an anaerobic anode system. The ability to generate current with an anode under aerobic conditions increases the potential applications and design options for microbial fuel cells.

  14. Degradation of toxaphene in water during anaerobic and aerobic conditions.

    Science.gov (United States)

    LacayoR, M; van Bavel, B; Mattiasson, B

    2004-08-01

    The degradation of technical toxaphene in water with two kinds of bioreactors operating in sequence was studied. One packed bed reactor was filled with Poraver (foam glass particles) running at anaerobic conditions and one suspended carrier biofilm reactor working aerobically. Chemical oxygen demand (COD), chloride, sulphate, pH, dissolved oxygen, total toxaphene and specific toxaphene isomers were measured. After 6 weeks approx. 87% of the total toxaphene was degraded reaching 98% by week 39. The majority of the conversion took place in the anaerobic reactor. The concentrations of toxaphene isomers with more chlorine substituents decreased more rapidly than for isomers with less chlorine substituents.

  15. Anaerobic Metabolism: Linkages to Trace Gases and Aerobic Processes

    Science.gov (United States)

    Megonigal, J. P.; Hines, M. E.; Visscher, P. T.

    2003-12-01

    Life evolved and flourished in the absence of molecular oxygen (O2). As the O2 content of the atmosphere rose to the present level of 21% beginning about two billion years ago, anaerobic metabolism was gradually supplanted by aerobic metabolism. Anaerobic environments have persisted on Earth despite the transformation to an oxidized state because of the combined influence of water and organic matter. Molecular oxygen diffuses about 104 times more slowly through water than air, and organic matter supports a large biotic O2 demand that consumes the supply faster than it is replaced by diffusion. Such conditions exist in wetlands, rivers, estuaries, coastal marine sediments, aquifers, anoxic water columns, sewage digesters, landfills, the intestinal tracts of animals, and the rumen of herbivores. Anaerobic microsites are also embedded in oxic environments such as upland soils and marine water columns. Appreciable rates of aerobic respiration are restricted to areas that are in direct contact with air or those inhabited by organisms that produce O2.Rising atmospheric O2 reduced the global area of anaerobic habitat, but enhanced the overall rate of anaerobic metabolism (at least on an area basis) by increasing the supply of electron donors and acceptors. Organic carbon production increased dramatically, as did oxidized forms of nitrogen, manganese, iron, sulfur, and many other elements. In contemporary anaerobic ecosystems, nearly all of the reducing power is derived from photosynthesis, and most of it eventually returns to O2, the most electronegative electron acceptor that is abundant. This photosynthetically driven redox gradient has been thoroughly exploited by aerobic and anaerobic microorganisms for metabolism. The same is true of hydrothermal vents (Tunnicliffe, 1992) and some deep subsurface environments ( Chapelle et al., 2002), where thermal energy is the ultimate source of the reducing power.Although anaerobic habitats are currently a small fraction of Earth

  16. Aerobic vs anaerobic exercise training effects on the cardiovascular system.

    Science.gov (United States)

    Patel, Harsh; Alkhawam, Hassan; Madanieh, Raef; Shah, Niel; Kosmas, Constantine E; Vittorio, Timothy J

    2017-02-26

    Physical exercise is one of the most effective methods to help prevent cardiovascular (CV) disease and to promote CV health. Aerobic and anaerobic exercises are two types of exercise that differ based on the intensity, interval and types of muscle fibers incorporated. In this article, we aim to further elaborate on these two categories of physical exercise and to help decipher which provides the most effective means of promoting CV health.

  17. Aerobic vs anaerobic exercise training effects on the cardiovascular system

    Science.gov (United States)

    Patel, Harsh; Alkhawam, Hassan; Madanieh, Raef; Shah, Niel; Kosmas, Constantine E; Vittorio, Timothy J

    2017-01-01

    Physical exercise is one of the most effective methods to help prevent cardiovascular (CV) disease and to promote CV health. Aerobic and anaerobic exercises are two types of exercise that differ based on the intensity, interval and types of muscle fibers incorporated. In this article, we aim to further elaborate on these two categories of physical exercise and to help decipher which provides the most effective means of promoting CV health. PMID:28289526

  18. Coupled anaerobic/aerobic biodegradation of 2,4,6 trichlorophenol

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Degradation of 2, 4, 6-trichlorophenol(TCP) with co-immobilizing anaerobic granular sludge and isolated aerobic bacterial specieswas studied in coupled anaerobic/aerobic integrated reactors. The synergism of aerobes and anaerobes within co-immobilized granule mightfacilitate degrading the TCP and exchange of anaerobic metabolites 4-CP, which promoted system organic removal efficiency and recovered fromorganic shock-loads more quickly. The biomass specific activities experiment further confirmed that strict anaerobes be not affected over thecourse of this experiment by the presence of an oxic environment, aerobic activity predominated in the outer co-immobilized granule layers,while the interior was characterized by anaerobic activity. The co-immobilized granule could thus enable both aerobic and anaerobic microbesfunction in the same reactor and thereby integrate the oxidative and reductive catabolism.

  19. Anaerobic growth of a "strict aerobe" (Bacillus subtilis).

    Science.gov (United States)

    Nakano, M M; Zuber, P

    1998-01-01

    There was a long-held belief that the gram-positive soil bacterium Bacillus subtilis is a strict aerobe. But recent studies have shown that B. subtilis will grow anaerobically, either by using nitrate or nitrite as a terminal electron acceptor, or by fermentation. How B. subtilis alters its metabolic activity according to the availability of oxygen and alternative electron acceptors is but one focus of study. A two-component signal transduction system composed of a sensor kinase, ResE, and a response regulator, ResD, occupies an early stage in the regulatory pathway governing anaerobic respiration. One of the essential roles of ResD and ResE in anaerobic gene regulation is induction of fnr transcription upon oxygen limitation. FNR is a transcriptional activator for anaerobically induced genes, including those for respiratory nitrate reductase, narGHJI.B. subtilis has two distinct nitrate reductases, one for the assimilation of nitrate nitrogen and the other for nitrate respiration. In contrast, one nitrite reductase functions both in nitrite nitrogen assimilation and nitrite respiration. Unlike many anaerobes, which use pyruvate formate lyase, B. subtilis can carry out fermentation in the absence of external electron acceptors wherein pyruvate dehydrogenase is utilized to metabolize pyruvate.

  20. Removal of Pesticides and Inorganic Contaminants in Anaerobic and Aerobic Biological Contactors

    Science.gov (United States)

    This presentation contains data on the removal of pesticides (acetochlor, clethodim, dicrotophos), ammonia, nitrate, bromate and perchlorate through aerobic and anaerobic biological treatment processes.

  1. STUDY OF AEROBIC AND ANAEROBIC BACTERIA IN CHRONIC SUPPURATIVE OTITIS MEDIA

    Directory of Open Access Journals (Sweden)

    Satyanarayana

    2015-04-01

    Full Text Available One hundred six patients with clinical diagnosis of CSOM were investigated bacteriologically using appropriate aerobic and anaerobic techniques. Positive cultures were obtained in 100 specimens. Aerobic bacteria alone were present in 51%, anaerobic bacteria alone in 10% and a combination of both in 34% of aural swabs . The anaerobes isolated were Bacteroides 30%, Peptostreptococci 26%, followed by others. Aerobic bacteriology showed the predominance of Pseudomonas 30.4% followed by Staphylococcus aureus 21.4%. Metronidazole was found to be most effective (87% drug aga inst anaerobes and Amikacin (98% against aerobes.

  2. Formation and functions of aerobic microbial granula; Entstehung und Funktionen aerober mikrobieller Granula

    Energy Technology Data Exchange (ETDEWEB)

    Etterer, T.; Wilderer, P.A. [Technische Univ. Muenchen, Garching (Germany). Lehrstuhl und Pruefamt fuer Wasserguete- und Abfallwirtschaft

    1999-07-01

    The present project investigates the phenomenon of the formation of aerobic microbial granula and their properties. To generate granula, sequencing batch reactors fed in batches were used. As shown by microbiological assays, fungi played an above-average role in granula formation and build-up. In first degradation experiments, furthermore, chemical oxygen demand (COD) could be reduced by over 90 %. The determination yielded comparable values to activated sludge, standing on average at 1.044g/ml. (orig.) [German] Im Rahmen des hier vorgestellten Projekts wurde das Phaenomen der Bildung aerober mikrobieller Granula sowie deren Eigenschaften untersucht. Zur Erzeugung von Granula wurden schubweise beschickte Reaktoren, sogenannte Sequencing-Batch-Reaktoren (SBR) verwendet. Wie mikrobiologische Untersuchungen zeigten spielen Pilze bei der Entstehung und beim Aufbau eine ueberdurchschnittliche Rolle. Des weiteren konnte in ersten Abbauversuchen der chemische Sauerstoff-Bedarf (CSB) um ueber 90% gesenkt werden. Die Dichtebestimmung ergab vergleichbare Werte zu Belebtschlamm und zwar im Durchschnitt 1,044 g/ml. (orig.)

  3. Aerobic and anaerobic incubation: Biological indexes of soil nitrogen availability

    Directory of Open Access Journals (Sweden)

    Kresović Mirjana M.

    2005-01-01

    Full Text Available Our researches have been made on brown forest soil that had been used in long-term experiments set up according to specified fertilization system for over 30 years. We have chosen those experiment variants in which quantities of nitrogen fertilizers were gradually increased. The soil samples taken from 0 cm to 30 cm depth were used to determine biological indexes of nitrogen availability (aerobic and anaerobic incubation. The same samples were also used for pot experiments with oat. Plant and soil parameters obtained in controlled conditions were used for determination of biological indexes reliability in measuring the soil nitrogen availability. On the grounds of correlation analysis, it can be concluded that biological index of nitrogen availability achieved by the anaerobic incubation (without substraction of the initial content of available nitrogen of the investigated brown forest soil is the reliable indicator of soil nitrogen availability. That is not the case with the aerobic incubation in which reliability has not been established.

  4. Aerobic and anaerobic exercise capacity in children with juvenile idiopathic arthritis

    NARCIS (Netherlands)

    van Brussel, Marco; Lelieveld, O T H M; van der Net, J; Engelbert, R H H; Helders, P J M; Takken, T

    2007-01-01

    Objective. To compare the aerobic and anaerobic exercise capacity of children with juvenile idiopathic arthritis (JIA) with healthy controls, to determine if there were differences based on disease onset type, and to examine the relationship between aerobic and anaerobic exercise capacity in childre

  5. Aerobic and anaerobic exercise capacity in adolescents with juvenile idiopathic arthritis

    NARCIS (Netherlands)

    Lelieveld, Otto; van Brussel, Marco; Takken, Tim; van Weert, Ellen; van Leeuwen, Miek A.; Armbrust, Wineke

    2007-01-01

    OBJECTIVE: To examine the aerobic and anaerobic exercise capacity in adolescents with juvenile idiopathic arthritis (JIA) compared with age- and sex-matched healthy individuals, and to assess associations between disease-related variables and aerobic and anaerobic exercise capacity. METHODS: Of 25 p

  6. Effects of anaerobic digestion and aerobic treatment on gaseous emissions from dairy manure storages

    Science.gov (United States)

    Effects of anaerobic digestion and aerobic treatment on the reduction of gaseous emissions from dairy manure storages were evaluated in this study. Screened dairy manure containing 3.5% volatile solids (VS) was either anaerobically digested or aerobically treated prior to storage in air-tight vessel...

  7. Anaerobic/aerobic treatment of selected azo dyes in wastewater

    Energy Technology Data Exchange (ETDEWEB)

    Seshadri, S.; Bishop, P.L. (Univ. of Cincinnati, OH (United States). Dept. of Civil and Environmental Engineering); Agha, A.M. (Univ. of Aleppo (Syrian Arab Republic). Faculty of Civil Engineering)

    1994-01-01

    Azo dyes represent the largest class of dyes in use today. Current environmental concern with these dyes revolves around the potential carcinogenic health risk presented by these dyes or their intermediate biodegradation products when exposed to microflora in the human digestive tract. These dyes may build up in the environment, since many wastewater treatment plants allow these dyes to pass through the system virtually untreated. The initial step in the degradation of these dyes is the cleavage of the Azo bond. This cleavage is often impossible under aerobic conditions, but has been readily demonstrated under anaerobic conditions. The focus of the study was to determine the feasibility of using an anaerobic fluidized-bed reactor to accomplish this cleavage. The effects of typical process variables such as hydraulic retention time (HRT), influent dye concentration levels, and degree of bed fluidization on removal efficiencies were also studied. The four dyes selected for this study were Acid-Orange 7, Acid-Orange 8, Acid-Orange 10, and Acid-Red 14. The effectiveness of using a bench-scale-activated sludge reactor as a sequenced second stage was also examined. Results indicate that nearly complete cleavage of the Azo bond is easily accomplished for each of the four dyes under hydraulic retention times of either 12 or 24 h. Initial results indicate, though, that aromatic amine by-products remain. The sequenced second stage was able to remove the remaining Chemical Oxygen Demand (COD) load to acceptable levels. Work is presently underway to determine the face of the anaerobic by-products in the aerobic second stage.

  8. Development of a specific anaerobic field test for aerobic gymnastics.

    Science.gov (United States)

    Alves, Christiano Robles Rodrigues; Borelli, Marcello Tadeu Caetano; Paineli, Vitor de Salles; Azevedo, Rafael de Almeida; Borelli, Claudia Cristine Gomes; Lancha Junior, Antônio Herbert; Gualano, Bruno; Artioli, Guilherme Giannini

    2015-01-01

    The current investigation aimed to develop a valid specific field test to evaluate anaerobic physical performance in Aerobic Gymnastics athletes. We first designed the Specific Aerobic Gymnast Anaerobic Test (SAGAT), which included gymnastics-specific elements performed in maximal repeated sprint fashion, with a total duration of 80-90 s. In order to validate the SAGAT, three independent sub-studies were performed to evaluate the concurrent validity (Study I, n=8), the reliability (Study II, n=10) and the sensitivity (Study III, n=30) of the test in elite female athletes. In Study I, a positive correlation was shown between lower-body Wingate test and SAGAT performance (Mean power: p = 0.03, r = -0.69, CI: -0.94 to 0.03 and Peak power: p = 0.02, r = -0.72, CI: -0.95 to -0.04) and between upper-body Wingate test and SAGAT performance (Mean power: p = 0.03, r = -0.67, CI: -0.94 to 0.02 and Peak power: p = 0.03, r = -0.69, CI: -0.94 to 0.03). Additionally, plasma lactate was similarly increased in response to SAGAT (p = 0.002), lower-body Wingate Test (p = 0.021) and a simulated competition (p = 0.007). In Study II, no differences were found between the time to complete the SAGAT in repeated trials (p = 0.84; Cohen's d effect size = 0.09; ICC = 0.97, CI: 0.89 to 0.99; MDC95 = 0.12 s). Finally, in Study III the time to complete the SAGAT was significantly lower during the competition cycle when compared to the period before the preparatory cycle (p Gymnastics training period. Taken together, these data have demonstrated that SAGAT is a specific, reliable and sensitive measurement of specific anaerobic performance in elite female Aerobic Gymnastics, presenting great potential to be largely applied in training settings.

  9. Modeling of vapor intrusion from hydrocarbon-contaminated sources accounting for aerobic and anaerobic biodegradation.

    Science.gov (United States)

    Verginelli, Iason; Baciocchi, Renato

    2011-11-01

    A one-dimensional steady state vapor intrusion model including both anaerobic and oxygen-limited aerobic biodegradation was developed. The aerobic and anaerobic layer thickness are calculated by stoichiometrically coupling the reactive transport of vapors with oxygen transport and consumption. The model accounts for the different oxygen demand in the subsurface required to sustain the aerobic biodegradation of the compound(s) of concern and for the baseline soil oxygen respiration. In the case of anaerobic reaction under methanogenic conditions, the model accounts for the generation of methane which leads to a further oxygen demand, due to methane oxidation, in the aerobic zone. The model was solved analytically and applied, using representative parameter ranges and values, to identify under which site conditions the attenuation of hydrocarbons migrating into indoor environments is likely to be significant. Simulations were performed assuming a soil contaminated by toluene only, by a BTEX mixture, by Fresh Gasoline and by Weathered Gasoline. The obtained results have shown that for several site conditions oxygen concentration below the building is sufficient to sustain aerobic biodegradation. For these scenarios the aerobic biodegradation is the primary mechanism of attenuation, i.e. anaerobic contribution is negligible and a model accounting just for aerobic biodegradation can be used. On the contrary, in all cases where oxygen is not sufficient to sustain aerobic biodegradation alone (e.g. highly contaminated sources), anaerobic biodegradation can significantly contribute to the overall attenuation depending on the site specific conditions.

  10. Modeling of vapor intrusion from hydrocarbon-contaminated sources accounting for aerobic and anaerobic biodegradation

    Science.gov (United States)

    Verginelli, Iason; Baciocchi, Renato

    2011-11-01

    A one-dimensional steady state vapor intrusion model including both anaerobic and oxygen-limited aerobic biodegradation was developed. The aerobic and anaerobic layer thickness are calculated by stoichiometrically coupling the reactive transport of vapors with oxygen transport and consumption. The model accounts for the different oxygen demand in the subsurface required to sustain the aerobic biodegradation of the compound(s) of concern and for the baseline soil oxygen respiration. In the case of anaerobic reaction under methanogenic conditions, the model accounts for the generation of methane which leads to a further oxygen demand, due to methane oxidation, in the aerobic zone. The model was solved analytically and applied, using representative parameter ranges and values, to identify under which site conditions the attenuation of hydrocarbons migrating into indoor environments is likely to be significant. Simulations were performed assuming a soil contaminated by toluene only, by a BTEX mixture, by Fresh Gasoline and by Weathered Gasoline. The obtained results have shown that for several site conditions oxygen concentration below the building is sufficient to sustain aerobic biodegradation. For these scenarios the aerobic biodegradation is the primary mechanism of attenuation, i.e. anaerobic contribution is negligible and a model accounting just for aerobic biodegradation can be used. On the contrary, in all cases where oxygen is not sufficient to sustain aerobic biodegradation alone (e.g. highly contaminated sources), anaerobic biodegradation can significantly contribute to the overall attenuation depending on the site specific conditions.

  11. Degradation of TCE using sequential anaerobic biofilm and aerobic immobilized bed reactor

    Science.gov (United States)

    Chapatwala, Kirit D.; Babu, G. R. V.; Baresi, Larry; Trunzo, Richard M.

    1995-01-01

    Bacteria capable of degrading trichloroethylene (TCE) were isolated from contaminated wastewaters and soil sites. The aerobic cultures were identified as Pseudomonas aeruginosa (four species) and Pseudomonas fluorescens. The optimal conditions for the growth of aerobic cultures were determined. The minimal inhibitory concentration values of TCE for Pseudomonas sps. were also determined. The aerobic cells were immobilized in calcium alginate in the form of beads. Degradation of TCE by the anaerobic and dichloroethylene (DCE) by aerobic cultures was studied using dual reactors - anaerobic biofilm and aerobic immobilized bed reactor. The minimal mineral salt (MMS) medium saturated with TCE was pumped at the rate of 1 ml per hour into the anaerobic reactor. The MMS medium saturated with DCE and supplemented with xylenes and toluene (3 ppm each) was pumped at the rate of 1 ml per hour into the fluidized air-uplift-type reactor containing the immobilized aerobic cells. The concentrations of TCE and DCE and the metabolites formed during their degradation by the anaerobic and aerobic cultures were monitored by GC. The preliminary study suggests that the anaerobic and aerobic cultures of our isolates can degrade TCE and DCE.

  12. Metal concentrations of simulated aerobic and anaerobic pilot scale landfill reactors

    Energy Technology Data Exchange (ETDEWEB)

    Sinan Bilgili, M. [Yildiz Technical University, Environmental Engineering Department, 34349 Yildiz, Besiktas, Istanbul (Turkey)]. E-mail: mbilgili@yildiz.edu.tr; Demir, Ahmet [Yildiz Technical University, Environmental Engineering Department, 34349 Yildiz, Besiktas, Istanbul (Turkey)]. E-mail: ahmetd@yildiz.edu.tr; Ince, Mahir [Department of Environmental Engineering, Gebze Institute of Technology, 41400 Gebze (Turkey)]. E-mail: mahirince@gyte.edu.tr; Ozkaya, Bestamin [Yildiz Technical University, Environmental Engineering Department, 34349 Yildiz, Besiktas, Istanbul (Turkey)]. E-mail: bozkaya@yildiz.edu.tr

    2007-06-25

    Leachate and solid waste samples from aerobic and anaerobic simulated landfill reactors operated with and without leachate recirculation were characterized in terms of metals such as Fe, Ca, K, Na, Cd, Cr, Cu, Pb, Ni, and Zn. Metal concentrations of aerobic landfill reactor leachate samples are always below the regulation limits. The higher concentrations in anaerobic landfill leachate samples decreased to regulation limits after the landfill becomes methanogenic. The effect of leachate recirculation is determined in anaerobic landfills more clearly than aerobic landfills. Metal precipitation resulted in a decrease in leachate metal content and an increase in solid waste metal content as expected. Result of the study show that the metal content of landfill leachate samples is not a major concern for both aerobic and anaerobic landfills.

  13. Aerobic and anaerobic organism productivity as factors that determine the level of physical health

    Directory of Open Access Journals (Sweden)

    Serorez T.B.

    2014-04-01

    Full Text Available Purpose : to identify and assess the level of physical health of men of the first coming of age 21-23 years. Material : the study involved 413 students and 17 teachers and sports doctors. Results : the influence of cross-country training aerobic and anaerobic focus on physical performance, aerobic and anaerobic performance of the student body. The efficiency impact exercises using running loads of aerobic and anaerobic focus on the physical health of students. It is proved that the level of aerobic performance drops to safe limits and requires correction. To correct the body's aerobic performance is recommended to use for running load. Conclusions: found that the correction would be the most efficient and effective through the development and implementation of health technologies based on the use of extracurricular classes jogging exercises. It should take into account the frequency and methods of learning, physical work mode power supply and energy value of each class.

  14. Complete remediation of PCE contaminated unsaturated soils by sequential anaerobic-aerobic bioventing.

    Science.gov (United States)

    Mihopoulos, P G; Suidan, M T; Sayles, G D

    2001-01-01

    Bioventing principles have been applied to completely dechlorinate tetrachloroethylene vapors in the unsaturated zone in a sequential anaerobic-aerobic pattern. The aerobic step yields trans-DCE and VC as PCE reductive dechlorination byproducts, while TCE and cis-DCE are observed as intermediates. The aerobic step results in rapid oxidation of the VC and trans-DCE to carbon dioxide. Hydrogen was delivered in the gas phase as a reducing agent for the anaerobic step at levels of 1%, and oxygen at 4.2% was used as an electron acceptor in the aerobic step. PCE and VC half lives in the anaerobic and aerobic steps respectively, where less than 10 min.

  15. Decolorization of azo dyes under batch anaerobic and sequential anaerobic/aerobic conditions.

    Science.gov (United States)

    Işik, Mustafa; Sponza, Delia Teresa

    2004-01-01

    Batch anaerobic and sequential anaerobic upflow anaerobic sludge blanket (UASB)/aerobic continuous stirred tank reactor (CSTR) were used to determine the color and COD removals under anaerobic/aerobic conditions. Two azo dyes namely "Reactive Black 5 (RB 5)," "Congo Red (CR)," and glucose as a carbon source were used for synthetic wastewater. The course of the decolorization process approximates to first order and zero order kinetics with respect to dye concentration for RB 5 and Congo Red azo dyes, respectively, in batch conditions. The decolorization kinetic constant (K0) values increased from 3.6 to 11.8 mg(L h)(-1) as increases in dye concentrations from 200 to 3200 mg L(-1) for CR. Increases in dye concentrations from 0 to 3200 mg L(-1) reduce the decolorization rate constant (k1) values from 0.0141 to 0.0019 h(-1) in batch studies performed with RB 5. Decolorization was achieved effectively under test conditions but ultimate decolorization of azo dyes was not observed at all dye concentrations in batch assay conditions. Dye concentrations of 100 mg L(-1) and 3000 mg L(-1) of glucose-COD containing basal medium were used for continuous studies. The effect of organic loadings and HRT, on the color removal efficiencies and methane gas productions were monitored. 94.1-45.4% COD and 79-73% color removal efficiencies were obtained at an organic system during decolorization of Reactive Black 5. 92.3-77.0% COD and 95.3-92.2% decolorization efficiencies were achieved at a organic loading rate of 1.03-6.65 kg (m3 day)(-1) and a HRT of 3.54-0.49 for Congo Red treatment. The results of this study showed that, although decolorization continued, COD removal efficiencies and methane gas production were depressed at high organic loadings under anaerobic conditions. Furthermore, VFA accumulation, alkalinity consumption, and methane gas percentage were monitored at organic loading as high as 2.49-4.74 kg (m3 day)(-1) and 24.60-30.62 kg (m3 day)(-1), respectively, through the

  16. Integrated Anaerobic-Aerobic Biodegradation of Multiple Contaminants Including Chlorinated Ethylenes, Benzene, Toluene, and Dichloromethane.

    Science.gov (United States)

    Yoshikawa, Miho; Zhang, Ming; Toyota, Koki

    2017-01-01

    Complete bioremediation of soils containing multiple volatile organic compounds (VOCs) remains a challenge. To explore the possibility of complete bioremediation through integrated anaerobic-aerobic biodegradation, laboratory feasibility tests followed by alternate anaerobic-aerobic and aerobic-anaerobic biodegradation tests were performed. Chlorinated ethylenes, including tetrachloroethylene (PCE), trichloroethylene (TCE), cis-dichloroethylene (cis-DCE), and vinyl chloride (VC), and dichloromethane (DCM) were used for anaerobic biodegradation, whereas benzene, toluene, and DCM were used for aerobic biodegradation tests. Microbial communities involved in the biodegradation tests were analyzed to characterize the major bacteria that may contribute to biodegradation. The results demonstrated that integrated anaerobic-aerobic biodegradation was capable of completely degrading the seven VOCs with initial concentration of each VOC less than 30 mg/L. Benzene and toluene were degraded within 8 days, and DCM was degraded within 20 to 27 days under aerobic conditions when initial oxygen concentrations in the headspaces of test bottles were set to 5.3% and 21.0%. Dehalococcoides sp., generally considered sensitive to oxygen, survived aerobic conditions for 28 days and was activated during the subsequent anaerobic biodegradation. However, degradation of cis-DCE was suppressed after oxygen exposure for more than 201 days, suggesting the loss of viability of Dehalococcoides sp., as they are the only known anaerobic bacteria that can completely biodegrade chlorinated ethylenes to ethylene. Anaerobic degradation of DCM following previous aerobic degradation was complete, and yet-unknown microbes may be involved in the process. The findings may provide a scientific and practical basis for the complete bioremediation of multiple contaminants in situ and a subject for further exploration.

  17. Relationships among traits of aerobic and anaerobic swimming performance in individual European sea bass Dicentrarchus labrax.

    Science.gov (United States)

    Marras, Stefano; Killen, Shaun S; Domenici, Paolo; Claireaux, Guy; McKenzie, David J

    2013-01-01

    Teleost fishes exhibit wide and temporally stable inter-individual variation in a suite of aerobic and anaerobic locomotor traits. One mechanism that could allow such variation to persist within populations is the presence of tradeoffs between aerobic and anaerobic performance, such that individuals with a high capacity for one type of performance have a reduced capacity for the other. We investigated this possibility in European seabass Dicentrarchuslabrax, each measured for a battery of indicators of maximum locomotor performance. Aerobic traits comprised active metabolic rate, aerobic scope for activity, maximum aerobic swimming speed, and stride length, using a constant acceleration test. Anaerobic traits comprised maximum speed during an escape response, maximum sprint speed, and maximum anaerobic burst speed during constant acceleration. The data provided evidence of significant variation in performance among individuals, but there was no evidence of any trade-offs among any traits of aerobic versus anaerobic swimming performance. Furthermore, the anaerobic traits were not correlated significantly among each other, despite relying on the same muscular structures. Thus, the variation observed may reflect trade-offs with other morphological, physiological or behavioural traits.

  18. Relationships among traits of aerobic and anaerobic swimming performance in individual European sea bass Dicentrarchus labrax.

    Directory of Open Access Journals (Sweden)

    Stefano Marras

    Full Text Available Teleost fishes exhibit wide and temporally stable inter-individual variation in a suite of aerobic and anaerobic locomotor traits. One mechanism that could allow such variation to persist within populations is the presence of tradeoffs between aerobic and anaerobic performance, such that individuals with a high capacity for one type of performance have a reduced capacity for the other. We investigated this possibility in European seabass Dicentrarchuslabrax, each measured for a battery of indicators of maximum locomotor performance. Aerobic traits comprised active metabolic rate, aerobic scope for activity, maximum aerobic swimming speed, and stride length, using a constant acceleration test. Anaerobic traits comprised maximum speed during an escape response, maximum sprint speed, and maximum anaerobic burst speed during constant acceleration. The data provided evidence of significant variation in performance among individuals, but there was no evidence of any trade-offs among any traits of aerobic versus anaerobic swimming performance. Furthermore, the anaerobic traits were not correlated significantly among each other, despite relying on the same muscular structures. Thus, the variation observed may reflect trade-offs with other morphological, physiological or behavioural traits.

  19. Active Female Maximal and Anaerobic Threshold Cardiorespiratory Responses to Six Different Water Aerobics Exercises

    Science.gov (United States)

    Antunes, Amanda H.; Alberton, Cristine L.; Finatto, Paula; Pinto, Stephanie S.; Cadore, Eduardo L.; Zaffari, Paula; Kruel, Luiz F. M.

    2015-01-01

    Purpose: Maximal tests conducted on land are not suitable for the prescription of aquatic exercises, which makes it difficult to optimize the intensity of water aerobics classes. The aim of the present study was to evaluate the maximal and anaerobic threshold cardiorespiratory responses to 6 water aerobics exercises. Volunteers performed 3 of the…

  20. The Impact of Temperature on Anaerobic Biological Perchlorate Removal and Aerobic Polishing of the Effluent

    Science.gov (United States)

    This abstract describes a pilot-scale evaluation of anaerobic biological perchlorate (C1O4) removal followed by aerobic effluent polishing. The anaerobic biological contactor operated for 3.5 years. During that period, two effluent polishing evaluations, lasting 311 an...

  1. The Impact of Temperature on Anaerobic Biological Perchlorate Removal and Aerobic Polishing of the Effluent - paper

    Science.gov (United States)

    This abstract describes a pilot-scale evaluation of anaerobic biological perchlorate (C1O4) removal followed by aerobic effluent polishing. The anaerobic biological contactor operated for 3.5 years. During that period, two effluent polishing evaluations, lasting 311 an...

  2. Biotransformation of nonylphenol ethoxylates during sewage treatment under anaerobic and aerobic conditions

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Biotransformation of nonylphenol ethoxylates (NPEOs) during continuous anaerobic sewage treatment was compared with the aerobic treatment of sewage spiked with 23 μmol/L technical NPEOs over a period of 90 d. Immediate degradation of NPEOs was observed under both anaerobic and aerobic conditions, indicating that the enzymes and bacteria required for NPEO degradation existed abundantly in both aerobic and anaerobic sludge. Both treatments achieved high removal (>92%) of the spiked NPEO9 mixture.Liquid chromatography-mass spectrometry (LC-MS) analysis showed that short-chain NPEOs (NPEO1-NPEO3) accumulated in anaerobic (2.01-2.56 μmol/L) and aerobic (1.62-2.03 μmol/L) effluents, with nonylphenol (NP) (0.24-0.31 μmol/L) as another group of metabolites in the anaerobic effluent, and nonylphenoxy carboxylates (NPECs) (2.79-3.30 μmol/L) in the aerobic effluent. Significant accumulation of NP in the anaerobic sludge and NPEO1-3 in the sludge of two reactors was observed. These results indicated that it was difficult to control these harmful metabolites in the conventional treatment processes. Denaturing gradient gel electrophoresis profiles of sludge samples support the speculation that the NPEO degradation bacteria might be the dominant indigenous species.

  3. Simultaneous anaerobic-aerobic biodegradation of halogenated phenolic compound under oxygen-limited conditions

    Institute of Scientific and Technical Information of China (English)

    CHEN Yuan-cai; LAN Hui-xia; ZHAN Huai-yu; FU Shi-yu

    2005-01-01

    The successful application of co-immobilized aerobic-anaerobic biomaes under limited aeration in wastewater treatment systems would eliminate the problems associated with the intermediates mono-chlorophenol(MCP) and di-chlorophenol(DCP) accumulations. With low initial pentachlorophenol(PCP) concentration, all PCP could be completely removed under oxygen-limited strict anaerobic conditions,and the removal efficiencies with different initial haedspace oxygen percentage(IHOP) were not obviously different from each other. While at high initial PCP concentration, under strictly anaerobic conditions PCP and their intermediates were clearly higher than that under other conditions, and produced obvious accumulation, the highest PCP reduction was achieved by the system receiving 30 IHOP, oxygen-limited system also exhibited lower residual TOC concentration and lower concentration of metabolic intermediates MCP and DCP. These results suggested that under strictly anaerobic condition the reductive dechlorination of low chlorinated compounde became rate limiting in the reductive dechlorination pathway, less chlorinated compounds be more amenable to aerobic degradation, and the aerobes of outer layers could function under limited oxygen. The co-immobilized aerobic-anaerobic biomass for methanogeneeis under limited-aeration for chlorophenol degradation might be an attractive and efficient altemative for the sequential anaerobic/aerobic system to achieve mineralization of a broad range of recalcitrance highly chlorinated organics and low final TOC concentrations.

  4. Influence of leachate recirculation on aerobic and anaerobic decomposition of solid wastes

    Energy Technology Data Exchange (ETDEWEB)

    Bilgili, M. Sinan [Yildiz Technical University, Environmental Engineering Department, 34349 Besiktas, Istanbul (Turkey)]. E-mail: mbilgili@yildiz.edu.tr; Demir, Ahmet [Yildiz Technical University, Environmental Engineering Department, 34349 Besiktas, Istanbul (Turkey)]. E-mail: ahmetd@yildiz.edu.tr; Ozkaya, Bestamin [Yildiz Technical University, Environmental Engineering Department, 34349 Besiktas, Istanbul (Turkey)]. E-mail: bozkaya@yildiz.edu.tr

    2007-05-08

    In this study, the effect of leachate recirculation on aerobic and anaerobic degradation of municipal solid wastes is determined by four laboratory-scale landfill reactors. The options studied and compared with the traditional anaerobic landfill are: leachate recirculation, landfill aeration, and aeration with leachate recirculation. Leachate quality is regularly monitored by the means of pH, alkalinity, total dissolved solids, conductivity, oxidation-reduction potential, chloride, chemical oxygen demand, ammonia, and total Kjeldahl nitrogen, in addition to generated leachate quantity. Aerobic leachate recirculated landfill appears to be the most effective option in the removal of organic matter and ammonia. The main difference between aerobic recirculated and non-recirculated landfill options is determined at leachate quantity. Recirculation is more effective on anaerobic degradation of solid waste than aerobic degradation. Further studies are going on to determine the optimum operational conditions for aeration and leachate recirculation rates, also with the operational costs of aeration and recirculation.

  5. The effect of cyclic anaerobic-aerobic conditions on biodegradation of azo dyes.

    Science.gov (United States)

    Yaşar, Semra; Cirik, Kevser; Cinar, Ozer

    2012-03-01

    The effect of cyclic anaerobic-aerobic conditions on the biodegradative capability of the mixed microbial culture for the azo dye Remazol Brilliant Violet 5R (RBV-5R) was investigated in the sequencing batch reactor (SBR) fed with a synthetic textile wastewater. The SBR had a 12-h cycle time with anaerobic-aerobic periods of 3/9, 6/6 and 9/3 h. General SBR performance was assessed by measurement of catabolic enzymes (catechol 2,3-dioxygenase, azo reductase), chemical oxygen demand (COD), color and amount of aromatic amines. In this study, under steady-state conditions, the anaerobic period of the cyclic SBR was found to allow the reductive decolorization of azo dye. Longer anaerobic periods resulted in higher color removal efficiencies, approximately 71% for the 3-h, 87% for 6-h and 92% for the 9-h duration. Total COD removal efficiencies were over 84% under each of the cyclic conditions and increased as the length of the anaerobic period was increased; however, the highest color removal rate was attained for the cycle with the shortest anaerobic period of 3 h. During the decolorization of RBV-5R, two sulfonated aromatic amines (benzene based and naphthalene based) were formed. Additionally, anaerobic azo reductase enzyme was found to be positively affected with the increasing duration of the anaerobic period; however; it was vice versa for the aerobic catechol 2,3-dioxygenase (C23DO) enzyme.

  6. Anaerobic and aerobic batch cultivations of Saccharomyces cerevisiae mutants impaired in glycerol synthesis

    DEFF Research Database (Denmark)

    Nissen, Torben Lauesgaard; Hamann, Claus Wendelboe; Kielland-Brandt, M. C.;

    2000-01-01

    Glycerol is formed as a by-product in production of ethanol and baker's yeast during fermentation of Saccharomyces cerevisiae under anaerobic and aerobic growth conditions, respectively. One physiological role of glycerol formation by yeast is to reoxidize NADH, formed in synthesis of biomass....... The modest effect of the GPD1 deletion under anaerobic conditions on the maximum specific growth rate and product yields clearly showed that Gdh2p is the important factor in glycerol formation during anaerobic growth. Strain TN6 (gpd1-Delta 1 gpd2-Delta 1) was unable to grow under anaerobic conditions due...

  7. COD fractions of leachate from aerobic and anaerobic pilot scale landfill reactors

    Energy Technology Data Exchange (ETDEWEB)

    Bilgili, M. Sinan [Yildiz Technical University, Environmental Engineering Department, 34349 Besiktas, Istanbul (Turkey)], E-mail: mbilgili@yildiz.edu.tr; Demir, Ahmet [Yildiz Technical University, Environmental Engineering Department, 34349 Besiktas, Istanbul (Turkey)], E-mail: ahmetd@yildiz.edu.tr; Akkaya, Ebru [Yildiz Technical University, Environmental Engineering Department, 34349 Besiktas, Istanbul (Turkey)], E-mail: ekoca@yildiz.edu.tr; Ozkaya, Bestamin [Yildiz Technical University, Environmental Engineering Department, 34349 Besiktas, Istanbul (Turkey)], E-mail: bozkaya@yildiz.edu.tr

    2008-10-01

    One of the most important problems with designing and maintaining a landfill is managing leachate that generated when water passes through the waste. In this study, leachate samples taken from aerobic and anaerobic landfill reactors operated with and without leachate recirculation are investigated in terms of biodegradable and non-biodegradable fractions of COD. The operation time is 600 days for anaerobic reactors and 250 days for aerobic reactors. Results of this study show that while the values of soluble inert COD to total COD in the leachate of aerobic landfill with leachate recirculation and aerobic dry reactors are determined around 40%, this rate was found around 30% in the leachate of anaerobic landfill with leachate recirculation and traditional landfill reactors. The reason for this difference is that the aerobic reactors generated much more microbial products. Because of this condition, it can be concluded that total inert COD/total COD ratios of the aerobic reactors were 60%, whereas those of anaerobic reactors were 50%. This study is important for modeling, design, and operation of landfill leachate treatment systems and determination of discharge limits.

  8. The degradability of biodegradable plastics in aerobic and anaerobic waste landfill model reactors.

    Science.gov (United States)

    Ishigaki, Tomonori; Sugano, Wataru; Nakanishi, Akane; Tateda, Masafumi; Ike, Michihiko; Fujita, Masanori

    2004-01-01

    Degradabilities of four kinds of commercial biodegradable plastics (BPs), polyhydroxybutyrate and hydroxyvalerate (PHBV) plastic, polycaprolactone plastic (PCL), blend of starch and polyvinyl alcohol (SPVA) plastic and cellulose acetate (CA) plastic were investigated in waste landfill model reactors that were operated as anaerobically and aerobically. The application of forced aeration to the landfill reactor for supplying aerobic condition could potentially stimulate polymer-degrading microorganisms. However, the individual degradation behavior of BPs under the aerobic condition was completely different. PCL, a chemically synthesized BP, showed film breakage under the both conditions, which may have contributed to a reduction in the waste volume regardless of aerobic or anaerobic conditions. Effective degradation of PHBV plastic was observed in the aerobic condition, though insufficient degradation was observed in the anaerobic condition. But the aeration did not contribute much to accelerate the volume reduction of SPVA plastic and CA plastic. It could be said that the recalcitrant portions of the plastics such as polyvinyl alcohol in SPVA plastic and the highly substituted CA in CA plastic prevented the BP from degradation. These results indicated existence of the great variations in the degradability of BPs in aerobic and anaerobic waste landfills, and suggest that suitable technologies for managing the waste landfill must be combined with utilization of BPs in order to enhance the reduction of waste volume in landfill sites.

  9. Positional Role Differences in the Aerobic and Anaerobic Power of Elite Basketball Players

    Directory of Open Access Journals (Sweden)

    Pojskić Haris

    2015-12-01

    Full Text Available The aim of the present study was to compare the aerobic and anaerobic power and capacity of elite male basketball players who played multiple positions. Fifty-five healthy players were divided into the following three different subsamples according to their positional role: guards (n = 22, forwards (n = 19 and centers (n = 14. The following three tests were applied to estimate their aerobic and anaerobic power and capacities: the countermovement jump (CMJ, a multistage shuttle run test and the Running-based Anaerobic Sprint Test (RAST. The obtained data were used to calculate the players’ aerobic and anaerobic power and capacities. To determine the possible differences between the subjects considering their different positions on the court, one-way analysis of variance (ANOVA with the Bonferroni post-hoc test for multiple comparisons was used. The results showed that there was a significant difference between the different groups of players in eleven out of sixteen measured variables. Guards and forwards exhibited greater aerobic and relative values of anaerobic power, allowing shorter recovery times and the ability to repeat high intensity, basketball-specific activities. Centers presented greater values of absolute anaerobic power and capacities, permitting greater force production during discrete tasks. Coaches can use these data to create more individualized strength and conditioning programs for different positional roles.

  10. Prospective study of the clinical performance of three BACTEC media in a modern emergency department: Plus Aerobic/F, Plus Anaerobic/F, and Anaerobic Lytic/F.

    Science.gov (United States)

    Rocchetti, Andrea; Di Matteo, Luigi; Bottino, Paolo; Foret, Benjamin; Gamalero, Elisa; Calabresi, Alessandra; Guido, Gianluca; Casagranda, Ivo

    2016-11-01

    The performance of 3 blood culture bottles (BACTEC Plus Aerobic/F, Plus Anaerobic/F, and Anaerobic Lytic/F) were analyzed with clinical specimens collected from 688 Emergency Department patients. A total of 270 strains belonging to 33 species were identified, with E. coli and S. aureus as the most frequently detected. Overall recovery rate (RR) of bacteria and yeast was equivalent in the Plus Aerobic/F vials (208 of 270 isolates; 77.0%) and Anaerobic Lytic/F vials (206 isolates; 76.3%) and significantly better than in the Plus Anaerobic/F vials (189 isolates; 70.0%). Median time to detection (TTD) was earliest with the Anaerobic Lytic/F vials (12.0h) compared with the Plus Aerobic/F (14.6h) and Plus Anaerobic/F vials (15.4h). Positivity rate (PR) was similar for Anaerobic Lytic/F vials (76.9%) and Plus Aerobic/F vials (76.5%), but better if compared with Plus Anaerobic/F vials (69.4%). The PR and TTD for the combination of Plus Aerobic/F with Anaerobic Lytic/F (94.5% and 12.3h, respectively) was significantly better than with Plus Aerobic/F with Plus Anaerobic/F (87.8% and 14.1h).

  11. Sequential anaerobic and aerobic treatment of pulp and paper mill effluent in pilot scale bioreactor.

    Science.gov (United States)

    Singh, Pratibha

    2007-01-01

    In the present study sequential anaerobic and aerobic treatment in two step bioreactor was performed for removal of colour in the pulp and paper mill effluent. In anaerobic treatment, colour 50%, lignin 62%, COD 29%, absordable organic halides (AOX) 25% and phenol 29% were reduced in eight days. The anaerobically treated effluent was separately applied in bioreactor in presence of fungal strain, Paecilomyces sp., and bacterial strain, Microbrevis luteum. Data of study indicated reduction in colour 80%, AOX 74%, lignin 81%, COD 93% and phenol 76 per cent by Paecilomyces sp. where as Microbrevis luteum showed removal in colour 59%, lignin 71%, COD 86%, AOX 84% and phenol 88% by day third when 7 days anaerobically treated effluent was further treated by aerobic microorganisms. Change in pH of the effluent and increase in biomass of microorganism's substantiated results of the study, which was concomitant to the treatment method.

  12. Bacterial community composition and abundance in leachate of semi-aerobic and anaerobic landfills

    Institute of Scientific and Technical Information of China (English)

    Wei Zhang; Bo Yue; Qi Wang; Zechun Huang; Qifei Huang; Zengqiang Zhang

    2011-01-01

    The abundance and phylogenetic composition of bacterial community in leachate of semi-aerobic and anaerobic landfill were compared through real-time polymerase chain reaction and denaturing gradient gel electrophoresis.In semi-aerobic landfill scenario,the bacterial 16S rRNA copy numbers in leachate had no significant reduction from initial stage to stable period.In the scenario of anaerobic landfill,the largest bacterial 16S rRNA gene copy number was found in leachate at initial stage,but it reduced significantly at stable period.Moreover,methane-oxidizing bacteria population in stable period was lower than that in initial period in both two landfill processes.However,semi-aerobic landfill leachate had more methanotrophic bacteria populations than that in the anaerobic one.Furthermore,according to the sequences and phylogenetic analysis,obvious difference could be detected in bacterial community composition in different scenarios.Proteobacteria and bacteroidetes took up a dominantly higher proportion in semi-aerobic landfill leachate.To summarize up,different landfill methods and its landfill ages had crucial impacts on bacterial abundance and composition in leachate of semi-aerobic and anaerobic landfills.

  13. The Effect of Anaerobic and Aerobic Fish Sludge Supernatant on Hydroponic Lettuce

    Directory of Open Access Journals (Sweden)

    Simon Goddek

    2016-06-01

    Full Text Available The mobilization of nutrients from fish sludge (i.e., feces and uneaten feed plays a key role in optimizing the resource utilization and thus in improving the sustainability of aquaponic systems. While several studies have documented the aerobic and anaerobic digestion performance of aquaculture sludge, the impact of the digestate on plant growth has yet to be understood. The present study examines the impact of either an aerobic or an anaerobic digestion effluent on lettuce plant growth, by enriching a mixture of aquaculture and tap water with supernatants from both aerobic and anaerobic batch reactors. The lettuce plants grown in the hydroponic system supplied with supernatant from an anaerobic reactor had significantly better performance with respect to weight gain than both, those in the system where supernatant from the aerobic reactor was added, as well as the control system. It can be hypothesized that this effect was caused by the presence of NH4+ as well as dissolved organic matter, plant growth promoting rhizobacteria and fungi, and humic acid, which are predominantly present in anaerobic effluents. This study should therefore be of value to researchers and practitioners wishing to further develop sludge remineralization in aquaponic systems.

  14. Aerobic and anaerobic power characteristics of competitive cyclists in the United States Cycling Federation.

    Science.gov (United States)

    Tanaka, H; Bassett, D R; Swensen, T C; Sampedro, R M

    1993-08-01

    The purpose of this study was to characterize the aerobic and anaerobic capabilities of United States Cycling Federation cyclists in different categories. To determine aerobic and anaerobic power, 38 competitive road cyclists (32 males, 6 females) performed a VO2max test and a Wingate anaerobic test, respectively. Male cyclists in category II had the highest VO2max, both in absolute and relative terms. Their VO2max was 6% and 10% higher than category III and IV cyclists, respectively (4.98 +/- 0.14 vs 4.72 +/- 0.15 vs 4.54 +/- 0.12 l/min). A significant difference existed between category II and IV male cyclists (p < 0.05). VO2max for female cyclists (3.37 +/- 0.13 l/min) was significantly (p < 0.05) lower than those for males. The Wingate anaerobic test revealed that male cyclists in category II also had the highest anaerobic power output. The peak power output in category II, III and IV was 13.86 +/- 0.23, 13.55 +/- 0.25, and 12.80 +/- 0.41 W/kg, respectively. The mean power output in category II, III, and IV was 11.22 +/- 0.18, 11.06 +/- 0.15, and 10.40 +/- 0.30 W/kg, respectively. The difference in the mean power output between category II and IV was significant (p < 0.05). Female cyclists recorded significantly less peak and mean power output than their male counterparts (p < 0.05). However, when expressed relative to lean body mass, anaerobic power was similar for both sexes. No inter-correlation was found in any measurement between the aerobic and anaerobic power values. On the whole, category II male cyclists were characterized by higher aerobic and anaerobic power outputs.(ABSTRACT TRUNCATED AT 250 WORDS)

  15. Decolorizing and detoxifying textile wastewater, containing both soluble and insoluble dyes, in a full scale combined anaerobic/aerobic system.

    Science.gov (United States)

    Frijters, C T M J; Vos, R H; Scheffer, G; Mulder, R

    2006-03-01

    The wastewater originating from the bleaching and dyeing processes in the textile factory Ten Cate Protect in Nijverdal (the Netherlands) was successfully treated in a sequential anaerobic/aerobic system. In the system, a combination of an anaerobic 70-m3 fluidized bed reactor and a 450-m3 aerobic basin with integrated tilted plate settlers, 80-95% of the color was removed. The color was largely removed in the preacidification basin and the anaerobic reactor. Color, deriving from both reactive as well as disperse, was anaerobically removed, indicating that these type of dyes were reduced to colorless products. Interestingly, the vat dyes, the anthraquinones and indigoids, which were thought to be removed mainly aerobically, were largely anaerobically decolorized. Apparently the anaerobic system is capable of effectively removing the color of both soluble as insoluble dyes. The treated effluent of the sequential anaerobic/aerobic treatment showed no toxicity towards the bioluminescent bacterium Vibrio fisheri (EC20 (95%) > 45%). Partially bypassing the anaerobic stage resulted in increased toxicity (EC20 (95%) of 9% and 14%) in the effluent of the aerobic treatment and caused significant decrease of color removal. The results of this study show a main contribution of anaerobic treatment in decolorizing and detoxifying the textile wastewater in the sequential anaerobic/aerobic system.

  16. Coupled anaerobic-aerobic treatment of whey wastewater in a sequencing batch reactor: proof of concept.

    Science.gov (United States)

    Frigon, J C; Bruneau, T; Moletta, R; Guiot, S R

    2007-01-01

    A proof of concept was performed in order to verify if the coupling of anaerobic and aerobic conditions inside the same digester could efficiently treat a reconstituted whey wastewater at 21 degrees C. The sequencing batch reactor (SBR) cycles combined initial anaerobic phase and final aerobic phase with reduced aeration. A series of 24 h cycles in 0.5 L digesters, with four different levels of oxygenation (none, 54, 108 and 182 mgO2 per gram of chemical oxygen demand (COD)), showed residual soluble chemical oxygen demand (sCOD) of 683 +/- 46, 720 +/- 33, 581 +/- 45, 1239 +/- 15 mg L(-1), respectively. Acetate and hydrogen specific activities were maintained for the anaerobic digester, but decreased by 10-25% for the acetate and by 20-50% for the hydrogen, in the coupled digesters. The experiment was repeated using 48 h cycles with limited aeration during 6 or 16 hours at 54 and 108 mgO2gCODinitial(-1), displaying residual sCOD of 177 +/- 43, 137 +/- 38, 104 +/- 22 and 112 +/- 9 mgL(-1) for the anaerobic and the coupled digesters, respectively. The coupled digesters recovered after a pH shock with residual sCOD as low as 132 mg L(-1) compared to 636 mg L(-1) for the anaerobic digester. With regard to the obtained results, the feasibility of the anaerobic-aerobic coupling in SBR digesters for the treatment of whey wastewater was demonstrated.

  17. Biodegradability and toxicity of sulphonate-based surfactants in aerobic and anaerobic aquatic environments.

    Science.gov (United States)

    García, M T; Campos, E; Marsal, A; Ribosa, I

    2009-02-01

    Four types of commonly used sulphonate-based surfactants (alkane sulphonates, alpha-olefin sulphonates, sulphosuccinates and methyl ester sulphonates) were tested for their aerobic and anaerobic biodegradability as well as for their toxicity to Daphnia magna and Photobacterium phosphoreum to assess the effect of the surfactant structure on those properties. Aerobic biodegradation was evaluated by means of the CO2 headspace test and anaerobic biodegradation was assessed by a method based on the ECETOC test. All the surfactants tested were readily biodegraded under aerobic conditions. No clear effect of the surfactant structures on the toxicity to the aquatic organisms tested was found. The most significant differences in the surfactants studied were observed in their behaviour under anaerobic conditions. Alkane sulphonates, alpha-olefin sulphonates and methyl ester sulphonates were not mineralized in lab anaerobic digesters despite the fact that the last one showed a certain degree of primary degradation. Nevertheless, these surfactants did not significantly inhibit methanogenic activity at concentrations up to 15 g surfactant/kg dry sludge, a concentration that is much higher than the expected concentrations of these surfactants in real anaerobic digesters. Sulphosuccinates showed a high level of primary biodegradation in anaerobic conditions. However, linear alkyl sulphosuccinates were completely mineralized whereas branched alkyl sulphosuccinates achieved percentages of ultimate biodegradation < or =50%.

  18. Decomposition Dynamics and Changes in Chemical Composition of Wheat Straw Residue under Anaerobic and Aerobic Conditions.

    Science.gov (United States)

    Gao, Hongjian; Chen, Xi; Wei, Junling; Zhang, Yajie; Zhang, Ligan; Chang, Jiang; Thompson, Michael L

    2016-01-01

    Soil aeration is a crucial factor that regulates crop residue decomposition, and the chemical composition of decomposing crop residues may change the forms and availability of soil nutrients, such as N and P. However, to date, differences in the chemical composition of crop straw residues after incorporation into soil and during its decomposition under anaerobic vs. aerobic conditions have not been well documented. The objective of the present study was to assess changes in the C-containing functional groups of wheat straw residue during its decomposition in anaerobic and aerobic environments. A 12-month incubation experiment was carried out to investigate the temporal variations of mass, carbon, and nitrogen loss, as well as changes in the chemical composition of wheat (Triticum aestivum L) straw residues under anaerobic and aerobic conditions by measuring C-containing functional groups using solid state nuclear magnetic resonance (NMR) spectroscopy. The residual mass, carbon content, and nitrogen content of the straw residue sharply declined during the initial 3 months, and then slowly decreased during the last incubation period from 3 to 12 months. The decomposition rate constant (k) for mass loss under aerobic conditions (0.022 d-1) was higher than that under anaerobic conditions (0.014 d-1). The residual mass percentage of cellulose and hemicellulose in the wheat straw gradually declined, whereas that of lignin gradually increased during the entire 12-month incubation period. The NMR spectra of C-containing functional groups in the decomposing straw under both aerobic and anaerobic conditions were similar at the beginning of the incubation as well as at 1 month, 6 months, and 12 months. The main alterations in C-containing functional groups during the decomposition of wheat straw were a decrease in the relative abundances of O-alkyl C and an increase in the relative abundances of alkyl C, aromatic C and COO/N-C = O functional groups. The NMR signals of alkyl C

  19. Inactivation of Mg Chelatase during Transition from Anaerobic to Aerobic Growth in Rhodobacter capsulatus

    OpenAIRE

    Willows, Robert D.; Lake, Vanessa; Roberts, Thomas Hugh; Beale, Samuel I.

    2003-01-01

    The facultative photosynthetic bacterium Rhodobacter capsulatus can adapt from an anaerobic photosynthetic mode of growth to aerobic heterotrophic metabolism. As this adaptation occurs, the cells must rapidly halt bacteriochlorophyll synthesis to prevent phototoxic tetrapyrroles from accumulating, while still allowing heme synthesis to continue. A likely control point is Mg chelatase, the enzyme that diverts protoporphyrin IX from heme biosynthesis toward the bacteriochlorophyll biosynthetic ...

  20. Studies on bacterial activities in aerobic and anaerobic waste water purification.

    Science.gov (United States)

    Adamse, A D; Deinema, M H; Zehnder, A J

    1984-01-01

    Some aspects of the bacteriology of aerobic and anaerobic waste water purification are discussed in view of current opinions and recent developments in the technology of waste water treatment. Various contributions of scientific workers attached to the Department of Microbiology of the Agricultural University, Wageningen, during the past 65 years are summarized. Besides, present investigations are described and research activities in future indicated.

  1. The Effect of Anaerobic and Aerobic Fish Sludge Supernatant on Hydroponic Lettuce

    NARCIS (Netherlands)

    Goddek, Simon; Schmautz, Zala; Scott, Ben; Delaide, Boris; Keesman, Karel; Wuertz, Sven; Junge, Ranka

    2016-01-01

    The mobilization of nutrients from fish sludge (i.e., feces and uneaten feed) plays a key role in optimizing the resource utilization and thus in improving the sustainability of aquaponic systems. While several studies have documented the aerobic and anaerobic digestion performance of aquaculture sl

  2. Investigating Aerobic, Anaerobic Combine Technical Trainings' Effects on Performance in Tennis Players

    Science.gov (United States)

    Suna, Gürhan; Kumartasli, Mehmet

    2017-01-01

    The aim of this study is to investigate eight-week aerobic, anaerobic combine technical trainings' effects on developments of performance. 21 athletes of tennis proficiency students from Sports Sciences Department were joined to the study voluntarily. Participated in the research athletes' ages' mean was 22,2 ± 0,3 year, lengths' mean was 177,3 ±…

  3. Heart rate recovery after aerobic and anaerobic tests: is there an influence of anaerobic speed reserve?

    Science.gov (United States)

    Del Rosso, Sebastián; Nakamura, Fabio Y; Boullosa, Daniel A

    2017-05-01

    The present study assessed if differences in the metabolic profile, inferred from the anaerobic speed reserve (ASR), would influence the dynamics of heart rate recovery (HRR) after two modes of exercise. Thirty-nine physical education students (14 females and 25 males) volunteered for this study. Participants carried out three separate testing sessions to assess maximal sprinting speed (MSS, 1st session), repeated sprint ability (RSA, 2nd session) and maximal aerobic speed (MAS) using the Université of Montreal Track Test (UMTT, 3rd session). ASR was defined as the difference between MSS and MAS. Heart rate was continuously registered throughout the tests and during the 5-min post-test recovery. To evaluate the influence of ASR on post-exercise, HRR comparisons between ASR-based groups [high ASR vs. low ASR] and sex groups (males vs. females) were performed. Significant differences (P < 0.05) were found between high ASR and low ASR groups of the same sex for indices of relative HRR after the RSA and UMTT. In addition, after the RSA test, males from the high ASR group had a significantly slower HRR kinetics compared with the males of the low ASR (P < 0.05) and the females of high ASR (P < 0.05); whereas females of the high ASR groups had a faster HRR kinetics compared with the females of low ASR group (P < 0.05). Our results showed that in males, post-exercise HRR could be related to the ASR, whereas in females, the influence of ASR is less clear.

  4. Pathogen inactivation in liquid dairy manure during anaerobic and aerobic digestions

    Science.gov (United States)

    Biswas, S.; Pandey, P.; Castillo, A. R.; Vaddella, V. K.

    2014-12-01

    Controlling manure-borne pathogens such as E. coli O157:H7, Salmonella spp. and Listeria monocytogenes are crucial for protecting surface and ground water as well as mitigating risks to human health. In California dairy farms, flushing of dairy manure (mainly animal feces and urine) from freestall barns and subsequent liquid-solid manure separation is a common practice for handling animal waste. The liquid manure fraction is generally pumped into the settling ponds and it goes into aerobic and/or anaerobic lagoons for extended period of time. Considering the importance of controlling pathogens in animal waste, the objective of the study was to understand the effects of anaerobic and aerobic digestions on the survival of three human pathogens in animal waste. The pathogen inactivation was assessed at four temperatures (30, 35, 42, and 50 °C), and the relationships between temperature and pathogen decay were estimated. Results showed a steady decrease of E. coli levels in aerobic and anaerobic digestion processes over the time; however, the decay rates varied with pathogens. The effect of temperature on Salmonella spp. and Listeria monocytogenes survival was different than the E. coli survival. In thermophilic temperatures (42 and 50 °C), decay rate was considerable greater compared to the mesophilic temperatures (30 and 35°C). The E. coli log reductions at 50 °C were 2.1 in both aerobic and anaerobic digestions after 13 days of incubation. The Salmonella spp. log reductions at 50 °C were 5.5 in aerobic digestion, and 5.9 in anaerobic digestion. The Listeria monocytogenes log reductions at 50 °C were 5.0 in aerobic digestion, and 5.6 in anaerobic digestion. The log reduction of E. coli, Salmonella spp., and Listeria monocytogens at 30 °C in aerobic environment were 0.1, 4.7, and 5.6, respectively. In anaerobic environment, the corresponding reductions were 0.4, 4.3, and 5.6, respectively. We anticipate that the outcomes of the study will help improving the

  5. Organic micropollutants in aerobic and anaerobic membrane bioreactors: Changes in microbial communities and gene expression

    KAUST Repository

    Harb, Moustapha

    2016-07-09

    Organic micro-pollutants (OMPs) are contaminants of emerging concern in wastewater treatment due to the risk of their proliferation into the environment, but their impact on the biological treatment process is not well understood. The purpose of this study is to examine the effects of the presence of OMPs on the core microbial populations of wastewater treatment. Two nanofiltration-coupled membrane bioreactors (aerobic and anaerobic) were subjected to the same operating conditions while treating synthetic municipal wastewater spiked with OMPs. Microbial community dynamics, gene expression levels, and antibiotic resistance genes were analyzed using molecular-based approaches. Results showed that presence of OMPs in the wastewater feed had a clear effect on keystone bacterial populations in both the aerobic and anaerobic sludge while also significantly impacting biodegradation-associated gene expression levels. Finally, multiple antibiotic-type OMPs were found to have higher removal rates in the anaerobic MBR, while associated antibiotic resistance genes were lower.

  6. Partition of aerobic and anaerobic swimming costs related to gait transitions in a labriform swimmer

    DEFF Research Database (Denmark)

    Svendsen, Jon Christian; Tudorache, Christian; Jordan, Anders D.;

    2010-01-01

    was to partition aerobic and anaerobic swimming costs at speeds below and above the Up–c in the striped surfperch Embiotoca lateralis using swimming respirometry and video analysis to test the hypothesis that the gait transition marks the switch from aerobic to anaerobic power output. Exercise oxygen consumption......Members of the family Embiotocidae exhibit a distinct gait transition from exclusively pectoral fin oscillation to combined pectoral and caudal fin propulsion with increasing swimming speed. The pectoral–caudal gait transition occurs at a threshold speed termed Up–c. The objective of this study...... included caudal fin propulsion in a mostly steady and unsteady (burst-assisted) mode, respectively. There was no evidence of EPOC after swimming at 1.4 and 1.9 L s–1, indicating that the pectoral–caudal gait transition was not a threshold for anaerobic metabolism. At 2.3 L s–1, E. lateralis switched...

  7. Aerobic and anaerobic microbial degradation of crude (4-methylcyclohexyl)methanol in river sediments.

    Science.gov (United States)

    Yuan, Li; Zhi, Wei; Liu, Yangsheng; Smiley, Elizabeth; Gallagher, Daniel; Chen, Xi; Dietrich, Andrea; Zhang, Husen

    2016-03-15

    Cyclohexane and some of its derivatives have been a major concern because of their significant adverse human health effects and widespread occurrence in the environment. The 2014 West Virginia chemical spill has raised public attention to (4-methylcyclohexyl)methanol (4-MCHM), one cyclohexane derivative, which is widely used in coal processing but largely ignored. In particular, the environmental fate of its primary components, cis- and trans-4-MCHM, remains largely unexplored. This study aimed to investigate the degradation kinetics and mineralization of cis- and trans-4-MCHM by sediment microorganisms under aerobic and anaerobic conditions. We found the removal of cis- and trans-4-MCHM was mainly attributed to biodegradation with little contribution from sorption. A nearly complete aerobic degradation of 4-MCHM occurred within 14 days, whereas the anaerobic degradation was reluctant with residual percentages of 62.6% of cis-4-MCHM and 85.0% of trans-4-MCHM after 16-day incubation. The cis-4-MCHM was degraded faster than the trans under both aerobic and anaerobic conditions, indicating an isomer-specific degradation could occur during the 4-MCHM degradation. Nitrate addition enhanced 4-MCHM mineralization by about 50% under both aerobic and anaerobic conditions. Both cis- and trans-4-MCHM fit well with the first-order kinetic model with respective degradation rates of 0.46-0.52 and 0.19-0.31 day(-)(1) under aerobic condition. Respective degradation rates of 0.041-0.095 and 0.013-0.052 day(-)(1) occurred under anaerobic condition. One bacterial strain capable of effectively degrading 4-MCHM isomers was isolated from river sediments and identified as Bacillus pumilus at the species level based on 16S rRNA gene sequence and 97% identity. Our findings will provide critical information for improving the prediction of the environmental fate of 4-MCHM and other cyclohexane derivatives with similar structure as well as enhancing the development of feasible treatment

  8. Effect of aerobic and anaerobic exercises on glycemiccontrol in type 1 diabetic youths

    Institute of Scientific and Technical Information of China (English)

    Andrea Lukács; László Barkai

    2015-01-01

    AIM: To evaluate the long-term effect of aerobic and/ or anaerobic exercise on glycemic control in youths with type 1 diabetes. METHODS: Literature review was performed in spring and summer 2014 using PubMed/MEDLINE, Google Scholar, Scopus, and ScienceDirect with the following terms: aerobic, anaerobic, high-intensity, resistance, exercise/training, combined with glycemic/metabolic control, glycated haemoglobin A1c (HbA1c) and type 1 diabetes. Only peer-reviewed articles in English wereincluded published in the last 15 years. It was selectedfrom 1999 to 2014. Glycemic control was measuredwith HbA1c. Studies with an intervention lasting atleast 12 wk were included if the HbA1c was measuredbefore and after the intervention.RESULTS: A total of nine articles were found, andthey were published between the years of 2002-2011.The sample size was 401 diabetic youths (166 malesand 235 females) with an age range of 10-19 yearsexcept one study, in which the age range was 13-30years. Study participants were from Australia, Tunisia,Lithuania, Taiwan, Turkey, Brazilia, Belgium, Egypt andFrance. Four studies were aerobic-based, four werecombined aerobic and anaerobic programs, and onecompared aerobic exercise to anaerobic one. Availablestudies had insufficient evidence that any type ofexercise or combined training would clearly improvethe glycemic control in type 1 diabetic youth. Onlythree (two aerobic-based and one combined) studiescould provide a significant positive change in glycemiccontrol.CONCLUSION: The regular physical exercise hasseveral other valuable physiological and health benefitsthat justify the inclusion of exercise in pediatricdiabetes treatment and care.

  9. Binary Interactions of Antagonistic Bacteria with Candida albicans Under Aerobic and Anaerobic Conditions.

    Science.gov (United States)

    Benadé, Eliska; Stone, Wendy; Mouton, Marnel; Postma, Ferdinand; Wilsenach, Jac; Botha, Alfred

    2016-04-01

    We used both aerobic and anaerobic liquid co-cultures, prepared with Luria Bertani broth, to study the effect of bacteria on the survival of Candida albicans in the external environment, away from an animal host. The bacteria were represented by Aeromonas hydrophila, Bacillus cereus, Bacillus subtilis, Clostridium, Enterobacter, Klebsiella pneumoniae, Kluyvera ascorbata and Serratia marcescens. Under aerobic conditions, the yeast's growth was inhibited in the presence of bacterial growth; however, under anaerobic conditions, yeast and bacterial growth in co-cultures was similar to that observed for pure cultures. Subsequent assays revealed that the majority of bacterial strains aerobically produced extracellular hydrolytic enzymes capable of yeast cell wall hydrolysis, including chitinases and mannan-degrading enzymes. In contrast, except for the A. hydrophila strain, these enzymes were not detected in anaerobic bacterial cultures, nor was the antimicrobial compound prodigiosin found in anaerobic cultures of S. marcescens. When we suspended C. albicans cells in crude extracellular enzyme preparations from K. pneumoniae and S. marcescens, we detected no negative effect on yeast viability. However, we found that these preparations enhance the toxicity of prodigiosin towards the yeast, especially in combination with mannan-degrading enzymes. Analyses of the chitin and mannan content of yeast cell walls revealed that less chitin was produced under anaerobic than aerobic conditions; however, the levels of mannan, known for its low permeability, remained the same. The latter phenomenon, as well as reduced production of the bacterial enzymes and prodigiosin, may contribute to anaerobic growth and survival of C. albicans in the presence of bacteria.

  10. Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Shihui [ORNL; Tschaplinski, Timothy J [ORNL; Engle, Nancy L [ORNL; Carroll, Sue L [ORNL; Martin, S L. [North Carolina State University; Davison, Brian H [ORNL; Palumbo, Anthony Vito [ORNL; Brown, Steven D [ORNL

    2009-01-01

    Zymomonas mobilis ZM4 (ZM4) produces near theoretical yields of ethanol with high specific productivity and recombinant strains are able to ferment both C-5 and C-6 sugars. Z. mobilis performs best under anaerobic conditions, but is an aerotolerant organism. However, the genetic and physiological basis of ZM4's response to various stresses is understood poorly. In this study, transcriptomic and metabolomic profiles for ZM4 aerobic and anaerobic fermentations were elucidated by microarray analysis and by high-performance liquid chromatography (HPLC), gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analyses. In the absence of oxygen, ZM4 consumed glucose more rapidly, had a higher growth rate, and ethanol was the major end-product. Greater amounts of other end-products such as acetate, lactate, and acetoin were detected under aerobic conditions and at 26 h there was only 1.7% of the amount of ethanol present aerobically as there was anaerobically. In the early exponential growth phase, significant differences in gene expression were not observed between aerobic and anaerobic conditions via microarray analysis. HPLC and GC analyses revealed minor differences in extracellular metabolite profiles at the corresponding early exponential phase time point. Differences in extracellular metabolite profiles between conditions became greater as the fermentations progressed. GC-MS analysis of stationary phase intracellular metabolites indicated that ZM4 contained lower levels of amino acids such as alanine, valine and lysine, and other metabolites like lactate, ribitol, and 4-hydroxybutanoate under anaerobic conditions relative to aerobic conditions. Stationary phase microarray analysis revealed that 166 genes were significantly differentially expressed by more than two-fold. Transcripts for Entner-Doudoroff (ED) pathway genes (glk, zwf, pgl, pgk, and eno) and gene pdc, encoding a key enzyme leading to ethanol production, were at least 30-fold more

  11. Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations

    Directory of Open Access Journals (Sweden)

    Palumbo Anthony V

    2009-01-01

    Full Text Available Abstract Background Zymomonas mobilis ZM4 (ZM4 produces near theoretical yields of ethanol with high specific productivity and recombinant strains are able to ferment both C-5 and C-6 sugars. Z. mobilis performs best under anaerobic conditions, but is an aerotolerant organism. However, the genetic and physiological basis of ZM4's response to various stresses is understood poorly. Results In this study, transcriptomic and metabolomic profiles for ZM4 aerobic and anaerobic fermentations were elucidated by microarray analysis and by high-performance liquid chromatography (HPLC, gas chromatography (GC and gas chromatography-mass spectrometry (GC-MS analyses. In the absence of oxygen, ZM4 consumed glucose more rapidly, had a higher growth rate, and ethanol was the major end-product. Greater amounts of other end-products such as acetate, lactate, and acetoin were detected under aerobic conditions and at 26 h there was only 1.7% of the amount of ethanol present aerobically as there was anaerobically. In the early exponential growth phase, significant differences in gene expression were not observed between aerobic and anaerobic conditions via microarray analysis. HPLC and GC analyses revealed minor differences in extracellular metabolite profiles at the corresponding early exponential phase time point. Differences in extracellular metabolite profiles between conditions became greater as the fermentations progressed. GC-MS analysis of stationary phase intracellular metabolites indicated that ZM4 contained lower levels of amino acids such as alanine, valine and lysine, and other metabolites like lactate, ribitol, and 4-hydroxybutanoate under anaerobic conditions relative to aerobic conditions. Stationary phase microarray analysis revealed that 166 genes were significantly differentially expressed by more than two-fold. Transcripts for Entner-Doudoroff (ED pathway genes (glk, zwf, pgl, pgk, and eno and gene pdc, encoding a key enzyme leading to ethanol

  12. Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions.

    Science.gov (United States)

    Tsuge, Yota; Hori, Yoshimi; Kudou, Motonori; Ishii, Jun; Hasunuma, Tomohisa; Kondo, Akihiko

    2014-10-01

    The toxic fermentation inhibitors in lignocellulosic hydrolysates raise serious problems for the microbial production of fuels and chemicals. Furfural is considered to be one of the most toxic compounds among these inhibitors. Here, we describe the detoxification of furfural in Corynebacterium glutamicum ATCC13032 under both aerobic and anaerobic conditions. Under aerobic culture conditions, furfuryl alcohol and 2-furoic acid were produced as detoxification products of furfural. The ratio of the products varied depending on the initial furfural concentration. Neither furfuryl alcohol nor 2-furoic acid showed any toxic effect on cell growth, and both compounds were determined to be the end products of furfural degradation. Interestingly, unlike under aerobic conditions, most of the furfural was converted to furfuryl alcohol under anaerobic conditions, without affecting the glucose consumption rate. Both the NADH/NAD(+) and NADPH/NADP(+) ratio decreased in the accordance with furfural concentration under both aerobic and anaerobic conditions. These results indicate the presence of a single or multiple endogenous enzymes with broad and high affinity for furfural and co-factors in C. glutamicum ATCC13032.

  13. Aerobic versus Anaerobic Microbial Degradation of Clothianidin under Simulated California Rice Field Conditions.

    Science.gov (United States)

    Mulligan, Rebecca A; Tomco, Patrick L; Howard, Megan W; Schempp, Tabitha T; Stewart, Davis J; Stacey, Phillip M; Ball, David B; Tjeerdema, Ronald S

    2016-09-28

    Microbial degradation of clothianidin was characterized under aerobic and anaerobic California rice field conditions. Rate constants (k) and half-lives (DT50) were determined for aerobic and anaerobic microcosms, and an enrichment experiment was performed at various nutrient conditions and pesticide concentrations. Temperature effects on anaerobic degradation rates were determined at 22 ± 2 and 35 ± 2 °C. Microbial growth was assessed in the presence of various pesticide concentrations, and distinct colonies were isolated and identified. Slow aerobic degradation was observed, but anaerobic degradation occurred rapidly at both 25 and 35 °C. Transformation rates and DT50 values in flooded soil at 35 ± 2 °C (k = -7.16 × 10(-2) ± 3.08 × 10(-3) day(-1), DT50 = 9.7 days) were significantly faster than in 25 ± 2 °C microcosms (k= -2.45 × 10(-2) ± 1.59 × 10(-3) day(-1), DT50 = 28.3 days). At the field scale, biodegradation of clothianidin will vary with extent of oxygenation.

  14. [Treatment of landfill leachate using sequential anaerobic/aerobic moving-bed biofilm reactor].

    Science.gov (United States)

    Chen, Sheng; Sun, De-zhi; Chen, Gui-xia; Jong, Shik Chung

    2006-10-01

    A sequential anaerobic-aerobic moving-bed biofilm reactor (MBBR) was employed to treat landfill leachate produced from disposal of municipal solid waste. The affecting operation conditions were investigated, and the affecting mechanism was also analyzed. The results showed that the HRT and organic loading rate (OLR) could greatly influence the treat efficiency. When the influent OLR was in the range of 4.01 - 7.87 kg/(m3 x d), the average total COD removal efficiency was 94.2%, and the contribution of anaerobic stage to total COD removal efficiency was 87.95% - 92.76%; while the influent OLR was in the range of 10.23-16.14 kg/(m3 x d), the average total COD removal efficiency was still 92.64%, and the contribution of anaerobic stage to total COD removal efficiency was 79.05% - 86.56%. As long as HRT of aerobic MBBR was longer than 1.25 d, the total HN4+ -N removal efficiency was persistently more than 97%; while the HRT was 0.75 d, the total HN4+ -N removal efficiency was only about 20%. The sequential anaerobic-aerobic MBBR can endure strong loading impact, even though the OLR was sharply increased 4 times and lasted for 24 h; the system could recover the normal treat efficiency in 3 d.

  15. Aerobic and Anaerobic Transformations in Estrogens and Nutrients in Swine Manure: Environmental Consequences

    Directory of Open Access Journals (Sweden)

    Ashok K. Singh

    2015-08-01

    Full Text Available Pig manure is an excellent fertilizer and rich source of organic carbon and nitrogen compounds such organic nitrogen (O-N (95% of total nitrogen that is plant-unavailable-nitrogen (PUN and mineralized nitrogen (about 1% of total nitrogen such as ammonium (NH4+ and nitrate (NO3 that are plant-available-nitrogen (PAN. In addition, manure also contains two forms of estrogens: (i poorly estrogenic thus essentially nontoxic conjugated estrogens (cEs such as estrone (cE1, estradiol (cE2 and estriol (cE3; and (ii highly estrogenic and toxic free estrogens (fEs such as fE2, fE1 and fE3. This study showed that aerobic processing reduced concentrations of total carbon (TC, O-N, PAN and NH4+/NH3 ratio, transiently hydrolyzed cEs (cE2 > cE1 > cE3 into corresponding fEs, transiently increased estrogenic activity and potential toxicity, and rapidly degraded fEs (fE2, fE1 > fE3, thus reducing the estrogenic activity in manure. Unlike aerobic processing, anaerobic processing stabilized and increased PAN and NH4+/NH3 ratio, thus increasing the manure’s fertilizer value. However, anaerobic processing, relative to aerobic processing, poorly hydrolyzed cEs (reducing transient toxicity and increasing reserve toxicity potential and poorly degraded fEs (increasing toxicity in manure. Thus, aerobic and anaerobic environments have distinct effects on manures’ PAN and estrogenic activity, presenting an interesting dilemma: anaerobic incubation that increases manures’ PAN does not effectively degrade estrogens, while aerobic incubation that effectively degrades estrogens (after transiently increasing their estrogenic activity also decreases PAN, thus making manure less profitable. New techniques are need to fully use manure as organic fertilizer.

  16. Investigating the nitrification and denitrification kinetics under aerobic and anaerobic conditions by Paracoccus denitrificans ISTOD1.

    Science.gov (United States)

    Medhi, Kristina; Singhal, Anjali; Chauhan, D K; Thakur, Indu Shekhar

    2017-03-16

    Municipal wastewater contains multiple nitrogen contaminants such as ammonia, nitrate and nitrite. Two heterotrophic nitrifier and aerobic denitrifiers, bacterial isolates ISTOD1 and ISTVD1 were isolated from domestic wastewater. On the basis of removal efficiency of ammonia, nitrate and nitrite under both aerobic and anaerobic conditions, ISTOD1 was selected and identified as Paracoccus denitrificans. Aerobically, NH4(+)-N had maximum specific nitrogen removal rate (Rxi) of 7.6g/gDCW/h and anaerobically, NO3(-)N showed Rxi of 2.5*10(-1)g/g DCW/h. Monod equation described the bioprocess kinetic coefficients, µmax and Ks, obtained by regression. Error functions were calculated to validate the Monod equation experimental data. Aerobic NO3(-)N showed the highest YW of 0.372mg DCW/mg NO3(-)N among the five conditions. ISTOD1 serves as a potential candidate for treating nitrogen rich wastewater using simultaneous nitrification and aerobic denitrification. It can be used in bioaugmentation studies under varied condition.

  17. Time-to-positivity-based discrimination between Enterobacteriaceae, Pseudomonas aeruginosa and strictly anaerobic Gram-negative bacilli in aerobic and anaerobic blood culture vials.

    Science.gov (United States)

    Defrance, Gilles; Birgand, Gabriel; Ruppé, Etienne; Billard, Morgane; Ruimy, Raymond; Bonnal, Christine; Andremont, Antoine; Armand-Lefèvre, Laurence

    2013-05-01

    Time-to-positivity (TTP) of first positive blood cultures growing Gram-negative bacilli (GNB) was investigated. When anaerobic vials were positive first, TTP ≤ 18 h differentiated Enterobacteriaceae from strict anaerobic Gram-negative bacilli (PPV 98.8%). When the aerobic ones were first, TTP ≤ 13 h differentiated Enterobacteriaceae from Pseudomonas aeruginosa and other GNB (PPV 80.8%).

  18. Degradation of 1-(2-chlorobenzoyl)-3-(4-chlorophenyl) urea by nanoscale zerovalent iron under aerobic and anaerobic conditions.

    Science.gov (United States)

    Lin, Haiying; Hou, Shaogang; Xie, Guohong; Yao, Ziwei; Zhou, Qingxiang

    2012-01-01

    The goal of present study was to investigate the applicability of nanoscale zerovalent iron (NZVI) on the degradation of 1-(2-chlorobenzoyl)-3-(4-chlorophenyl) urea (CCU) under aerobic and anaerobic conditions, respectively. The experimental results showed that NZVI could effectively degrade CCU, but the removal efficiencies were different under these two different conditions. The best removal efficiencies for CCU were 90.2 and 75.8% under aerobic and anaerobic conditions, respectively. The highly oxidative hydroxyl radicals and reductively hydrogen would account for the rapid degradation of CCU under aerobic and anaerobic conditions, respectively. The experimental results also showed that surfactant Tween 20 significantly inhibited the degradation of CCU under aerobic conditions. However it markedly enhanced the degradation of CCU under anaerobic condition.

  19. On improving effect combining aerobic and anaerobic loads of character in the classroom for physical training in universities

    Directory of Open Access Journals (Sweden)

    Goginava S.E.

    2014-06-01

    Full Text Available Purpose: substantiate the health effects on the body through a combination of students in the classroom for physical training loads of aerobic and anaerobic nature. Material : the study involved 174 students of 1-3 courses NIU "BSU." Of these, 90 girls and 84 boys aged 17-20 years. Results : the specificity of the health effects of athletic gymnastics as a means of anaerobic training students. The specificity of the health effects of swimming as a means of improving aerobic exercise. Expediency and the method of load combination of aerobic and anaerobic focus on employment in physical education with students. Conclusions: results of scientific studies show that the load combination of aerobic and anaerobic characters on physical education classes at the university promotes the significant increase in the level of physical health of students of the basic training department.

  20. An evaluation of aerobic and anaerobic composting of banana peels treated with different inoculums for soil nutrient replenishment.

    Science.gov (United States)

    Kalemelawa, Frank; Nishihara, Eiji; Endo, Tsuneyoshi; Ahmad, Zahoor; Yeasmin, Rumana; Tenywa, Moses M; Yamamoto, Sadahiro

    2012-12-01

    This study sought to evaluate the efficacy of aerobic and anaerobic composting of inoculated banana peels, and assess the agronomic value of banana peel-based compost. Changes in the chemical composition under aerobic and anaerobic conditions were examined for four formulations of banana peel-based wastes over a period of 12 weeks. The formulations i.e. plain banana peel (B), and a mixture with either cow dung (BC), poultry litter (BP) or earthworm (BE) were separately composted under aerobic and anaerobic conditions under laboratory conditions. Inoculation with either cow dung or poultry litter significantly facilitated mineralization in the order: BP>BC>B. The rate of decomposition was significantly faster under aerobic than in anaerobic composting conditions. The final composts contained high K (>100 g kg(-1)) and TN (>2%), indicating high potential as a source of K and N fertilizer.

  1. [Aerobic and aero-anaerobic bacterial flora of the nasal cavities of lambs].

    Science.gov (United States)

    Menoueri, N; Richard, Y; Brunet, J; Oudar, J

    1988-01-01

    On the basis of bacteriological examinations carried out on 415 intranasal swabs, aerobic and aero-anaerobic respiratory microbes were studied in lambs. A great diversity of bacteria has been found (in total 35 bacterial species and genus have been identified). This flora is characterised by the presence of gram positive cocci with a gram negative strain included in genus Moraxella and connected with Moraxella bovis species.

  2. Effects of aerobic-anaerobic transient conditions on sulfur and metal cycles in sewer biofilms

    NARCIS (Netherlands)

    Nielsen, A.; Lens, P.N.L.; Vollertsen, J.; Hvitved-Jacobsen, Th.

    2005-01-01

    Interactions between sulfur and metals were studied in aerobic and anaerobic biofilms grown on domestic waste water at 15°C. The dominant metals in the waste water were iron, zinc and copper, which were present in average concentrations of 0.5mg/l, 0.6mg/l and 0.1m/l, respectively. Copper and zinc w

  3. Simulation of aerobic and anaerobic biodegradation processes at a crude oil spill site

    Science.gov (United States)

    Essaid, Hedeff I.; Bekins, Barbara A.; Godsy, E. Michael; Warren, Ean; Baedecker, Mary Jo; Cozzarelli, Isabelle M.

    1995-01-01

    A two-dimensional, multispecies reactive solute transport model with sequential aerobic and anaerobic degradation processes was developed and tested. The model was used to study the field-scale solute transport and degradation processes at the Bemidji, Minnesota, crude oil spill site. The simulations included the biodegradation of volatile and nonvolatile fractions of dissolved organic carbon by aerobic processes, manganese and iron reduction, and methanogenesis. Model parameter estimates were constrained by published Monod kinetic parameters, theoretical yield estimates, and field biomass measurements. Despite the considerable uncertainty in the model parameter estimates, results of simulations reproduced the general features of the observed groundwater plume and the measured bacterial concentrations. In the simulation, 46% of the total dissolved organic carbon (TDOC) introduced into the aquifer was degraded. Aerobic degradation accounted for 40% of the TDOC degraded. Anaerobic processes accounted for the remaining 60% of degradation of TDOC: 5% by Mn reduction, 19% by Fe reduction, and 36% by methanogenesis. Thus anaerobic processes account for more than half of the removal of DOC at this site.

  4. Characterization of biofoulants illustrates different membrane fouling mechanisms for aerobic and anaerobic membrane bioreactors

    KAUST Repository

    Xiong, Yanghui

    2015-11-17

    This study compares the membrane fouling mechanisms of aerobic (AeMBR) and anaerobic membrane bioreactors (AnMBR) of the same reactor configuration at similar operating conditions. Although both the AeMBR and AnMBR achieved more than 90% COD removal efficiency, the fouling mechanisms were different. Molecular weight (MW) fingerprint profiles showed that a majority of fragments in anaerobic soluble microbial products (SMP) were retained by the membrane and some fragments were present in both SMP and in soluble extracellular polymeric substances (EPS), suggesting that the physical retention of SMP components contributed to the AnMBR membrane fouling. One of the dominant fragments was comprised of glycoliproprotein (size 630-640 kD) and correlated in abundance in AnMBR-EPS with the extent of anaerobic membrane fouling. In contrast, all detected AeMBR-SMP fragments permeated through the membrane. Aerobic SMP and soluble EPS also showed very different fingerprinting profiles. A large amount of adenosine triphosphate was present in the AeMBR-EPS, suggesting that microbial activity arising from certain bacterial populations, such as unclassified Comamonadaceae and unclassified Chitinophagaceae, may play a role in aerobic membrane fouling. This study underlines the differences in fouling mechanisms between AeMBR and AnMBR systems and can be applied to facilitate the development of appropriate fouling control strategies.

  5. ATP content and biomass activity in sequential anaerobic/aerobic reactors

    Institute of Scientific and Technical Information of China (English)

    陈红

    2004-01-01

    Specific ATP content of volatile solids was measured to characterize the sludge activity in a sequential anaerobic/aerobic wastewater treatment system, with an upflow anaerobic sludge blanket (UASB) reactor and a three-phase aerobic fluidized bed (AFB) reactor. The wastewater COD level was 2000(3000 mg/L in simulation of real textile wastewater. The ATP content and the specific ATP contents of volatile solids at different heights of the UASB reactor and those of the suspended and immobilized biomass in the AFB reactor were measured. In the UASB reactor, the maximum value of specific ATP (0.85 mg ATP/g VS) was obtained at a hydraulic retention time (HRT) 7.14 h in the blanket solution. In the AFB reactor, the specific ATP content of suspended biomass was higher than that of immobilized biomass and increased with hydraulic retention time reaching a maximum value of 1.6 mg ATP/g VS at hydraulic retention time 4.35 h. The ATP content of anaerobes in the UASB effluent declined rapidly under aerobic conditions following a 2nd-order kinetic model.

  6. Kinetics and thermodynamics of biodegradation of hydrolyzed polyacrylamide under anaerobic and aerobic conditions.

    Science.gov (United States)

    Zhao, Lanmei; Bao, Mutai; Yan, Miao; Lu, Jinren

    2016-09-01

    Kinetics and thermodynamics of hydrolyzed polyacrylamide (HPAM) biodegradation in anaerobic and aerobic activated sludge biochemical treatment systems were explored to determine the maximum rate and feasibility of HPAM biodegradation. The optimal nutrient proportions for HPAM biodegradation were determined to be 0.08g·L(-1) C6H12O6, 1.00g·L(-1) NH4Cl, 0.36g·L(-1) NaH2PO4 and 3.00g·L(-1) K2HPO4 using response surface methodology (RSM). Based on the kinetics, the maximum HPAM biodegradation rates were 16.43385mg·L(-1)·d(-1) and 2.463mg·L(-1)·d(-1) in aerobic and anaerobic conditions, respectively. The activation energy (Ea) of the aerobic biodegradation was 48.9897kJ·mol(-1). Entropy changes (ΔS) of biochemical treatment system decreased from 216.21J·K(-1) to 2.39J·K(-1). Thermodynamic windows of opportunity for HPAM biodegradation were drawn. And it demonstrated HPAM was biodegraded into acetic acid and CO2 under laboratory conditions. Growth-process equations for functional bacteria anaerobically grown on polyacrylic acid were constructed and it confirmed electron equivalence between substrate and product.

  7. Comparison of the transport and deposition of Pseudomonas aeruginosa under aerobic and anaerobic conditions

    Science.gov (United States)

    Zhang, Huixin; Zeng, Hongbo; Ulrich, Ania C.; Liu, Yang

    2016-02-01

    Laboratory-scale columns were employed to study the effect of oxygen and ionic strength on the transport of Pseudomonas aeruginosa PAO1 in porous media. In anaerobic experiments, cells were grown and transport experiments were conducted in a well-controlled anaerobic chamber. Cell surface electrokinetic potentials were measured and surface elemental composition was analyzed using X-ray photoelectron spectroscopy (XPS). Transport experimental results showed reduced travel distance of PAO1 with increased ionic strength under aerobic and anaerobic conditions, consistent with calculated Derjaguin-Landau-Verwey-Overbeek (DLVO) theory. The deposition rates of PAO1 were significantly higher in aerobic than in anaerobic condition at higher ionic strength (10 and 100 mM), although the electrokinetic potentials were similar throughout the tested ionic strength (1, 10, and 100 mM). No difference in PAO1 deposition rate was observed at 1 mM. XPS analysis showed that variation in cell surface composition due to different growth conditions played a primary role in determining the different deposition behaviors.

  8. Biodegradability of biodegradable/degradable plastic materials under aerobic and anaerobic conditions.

    Science.gov (United States)

    Mohee, R; Unmar, G D; Mudhoo, A; Khadoo, P

    2008-01-01

    A study was conducted on two types of plastic materials, Mater-Bi Novamont (MB) and Environmental Product Inc. (EPI), to assess their biodegradability under aerobic and anaerobic conditions. For aerobic conditions, organic fractions of municipal solid wastes were composted. For the anaerobic process, anaerobic inoculum from a wastewater treatment plant was used. Cellulose filter papers (CFP) were used as a positive control for both mediums. The composting process was monitored in terms of temperature, moisture and volatile solids and the biodegradation of the samples were monitored in terms of mass loss. Monitoring results showed a biodegradation of 27.1% on a dry basis for MB plastic within a period of 72 days of composting. Biodegradability under an anaerobic environment was monitored in terms of biogas production. A cumulative methane gas production of 245 ml was obtained for MB, which showed good degradation as compared to CFP (246.8 ml). However, EPI plastic showed a cumulative methane value of 7.6 ml for a period of 32 days, which was close to the blank (4.0 ml). The EPI plastic did not biodegrade under either condition. The cumulative carbon dioxide evolution after 32 days was as follows: CFP 4.406 cm3, MB 2.198 cm3 and EPI 1.328 cm3. The cumulative level of CO2 varying with time fitted sigmoid type curves with R2 values of 0.996, 0.996 and 0.995 for CFP, MB and EPI, respectively.

  9. Assessment of Maximum Aerobic Capacity and Anaerobic Threshold of Elite Ballet Dancers.

    Science.gov (United States)

    Wyon, Matthew A; Allen, Nick; Cloak, Ross; Beck, Sarah; Davies, Paul; Clarke, Frances

    2016-09-01

    An athlete's cardiorespiratory profile, maximal aerobic capacity, and anaerobic threshold is affected by training regimen and competition demands. The present study aimed to ascertain whether there are company rank differences in maximal aerobic capacity and anaerobic threshold in elite classical ballet dancers. Seventy-four volunteers (M 34, F 40) were recruited from two full-time professional classical ballet companies. All participants completed a continuous incremental treadmill protocol with a 1-km/hr speed increase at the end of each 1-min stage until termination criteria had been achieved (e.g., voluntary cessation, respiratory exchange ratio <1.15, HR ±5 bpm of estimated HRmax). Peak VO2 (5-breathe smooth) was recorded and anaerobic threshold calculated using ventilatory curve and ventilatory equivalents methods. Statistical analysis reported between-subject effects for gender (F1,67=35.18, p<0.001) and rank (F1,67=8.67, p<0.001); post hoc tests reported soloists (39.5±5.15 mL/kg/min) as having significantly lower VO2 peak than artists (45.9±5.75 mL/kg/min, p<0.001) and principal dancers (48.07±3.24 mL/kg/min, p<0.001). Significant differences in anaerobic threshold were reported for age (F1,67=7.68, p=0.008) and rank (F1,67=3.56, p=0.034); post hoc tests reported artists (75.8±5.45%) having significantly lower anaerobic threshold than soloists (80.9±5.71, p<0.01) and principals (84.1±4.84%, p<0.001). The observed differences in VO2 peak and anaerobic threshold between the ranks in ballet companies are probably due to the different rehearsal and performance demands.

  10. Two-stage anaerobic and post-aerobic mesophilic digestion of sewage sludge: Analysis of process performance and hygienization potential.

    Science.gov (United States)

    Tomei, M Concetta; Mosca Angelucci, Domenica; Levantesi, Caterina

    2016-03-01

    Sequential anaerobic-aerobic digestion has been demonstrated to be effective for enhanced sludge stabilization, in terms of increased solid reduction and improvement of sludge dewaterability. In this study, we propose a modified version of the sequential anaerobic-aerobic digestion process by operating the aerobic step under mesophilic conditions (T=37 °C), in order to improve the aerobic degradation kinetics of soluble and particulate chemical oxygen demand (COD). Process performance has been assessed in terms of "classical parameters" such as volatile solids (VS) removal, biogas production, COD removal, nitrogen species, and polysaccharide and protein fate. The aerobic step was operated under intermittent aeration to achieve nitrogen removal. Aerobic mesophilic conditions consistently increased VS removal, providing 32% additional removal vs. 20% at 20 °C. Similar results were obtained for nitrogen removal, increasing from 64% up to 99% at the higher temperature. Improved sludge dewaterability was also observed with a capillary suction time decrease of ~50% during the mesophilic aerobic step. This finding may be attributable to the decreased protein content in the aerobic digested sludge. The post-aerobic digestion exerted a positive effect on the reduction of microbial indicators while no consistent improvement of hygienization related to the increased temperature was observed. The techno-economic analysis of the proposed digestion layout showed a net cost saving for sludge disposal estimated in the range of 28-35% in comparison to the single-phase anaerobic digestion.

  11. Individual variation and repeatability in aerobic and anaerobic swimming performance of European sea bass, Dicentrarchus labrax.

    Science.gov (United States)

    Marras, S; Claireaux, G; McKenzie, D J; Nelson, J A

    2010-01-01

    Studies of inter-individual variation in fish swimming performance may provide insight into how selection has influenced diversity in phenotypic traits. We investigated individual variation and short-term repeatability of individual swimming performance by wild European sea bass in a constant acceleration test (CAT). Fish were challenged with four consecutive CATs with 5 min rest between trials. We measured maximum anaerobic speed at exhaustion (U(CAT)), gait transition speed from steady aerobic to unsteady anaerobic swimming (U(gt)), routine metabolic rate (RMR), post-CAT maximum metabolic rate (MMR), aerobic scope and recovery time from the CATs. Fish achieved significantly higher speeds during the first CAT (U(CAT)=170 cm s(-1)), and had much more inter-individual variation in performance (coefficient of variation, CV=18.43%) than in the subsequent three tests (U(CAT)=134 cm s(-1); CV=7.3%), which were very repeatable among individuals. The individual variation in U(CAT) in the first trial could be accounted for almost exclusively by variation in anaerobic burst-and-coast performance beyond U(gt). The U(gt) itself varied substantially between individuals (CV=11.4%), but was significantly repeatable across all four trials. Individual RMR and MMR varied considerably, but the rank order of post-CAT MMR was highly repeatable. Recovery rate from the four CATs was highly variable and correlated positively with the first U(CAT) (longer recovery for higher speeds) but negatively with RMR and aerobic scope (shorter recovery for higher RMR and aerobic scope). This large variation in individual performance coupled with the strong correlations between some of the studied variables may reflect divergent selection favouring alternative strategies for foraging and avoiding predation.

  12. Role of aerobic and anaerobic circular mantle muscle fibers in swimming squid: electromyography.

    Science.gov (United States)

    Bartol, I K

    2001-02-01

    Circular mantle muscle of squids and cuttlefishes consists of distinct zones of aerobic and anaerobic muscle fibers that are thought to have functional roles analogous to red and white muscle in fishes. To test predictions of the functional role of the circular muscle zones during swimming, electromyograms (EMGs) in conjunction with video footage were recorded from brief squid Lolliguncula brevis (5.0-6.8 cm dorsal mantle length, 10.9-18.3 g) swimming in a flume at speeds of 3-27 cm s(-1). In one set of experiments, in which EMGs were recorded from electrodes intersecting both the central anaerobic and peripheral aerobic circular mantle muscles, electrical activity was detected during each mantle contraction at all swimming speeds, and the amplitude and frequency of responses increased with speed. In another set of experiments, in which EMGs were recorded from electrodes placed in the central anaerobic circular muscle fibers alone, electrical activity was not detected during mantle contraction until speeds of about 15 cm s(-1), when EMG activity was sporadic. At speeds greater than 15 cm s(-1), the frequency of central circular muscle activity subsequently increased with swimming speed until maximum speeds of 21-27 cm s(-1), when muscular activity coincided with the majority of mantle contractions. These results indicate that peripheral aerobic circular muscle is used for low, intermediate, and probably high speeds, whereas central anaerobic circular muscle is recruited at intermediate speeds and used progressively more with speed for powerful, unsteady jetting. This is significant because it suggests that there is specialization and efficient use of locomotive muscle in squids.

  13. Central treatment of different emulsion wastewaters by an integrated process of physicochemically enhanced ultrafiltration and anaerobic-aerobic biofilm reactor.

    Science.gov (United States)

    Zhang, Weijun; Xiao, Ping; Wang, Dongsheng

    2014-05-01

    The feasibility of an integrated process of ultrafiltration (UF) enhanced by combined chemical emulsion breaking with vibratory shear and anaerobic/aerobic biofilm reactor for central treatment of different emulsion wastewaters was investigated. Firstly, it was found that calcium chloride exhibited better performance in oil removal than other inorganic salts. Chemical demulsification pretreatment could efficiently improve oil removal and membrane filtration in emulsion wastewater treatment by VSEP. According to aerobic batch bioassay, UF permeate exhibited good biodegradability and could be further treated with biological process. Additionally, pilot test indicated that anaerobic-aerobic biofilm exhibited an excellent ability against rise in organic loading and overall chemical oxygen demand (COD) removal efficiency of biological system was more than 93% of which 82% corresponded to the anaerobic process and 11% to the aerobic degradation. The final effluent of integrated process could meet the "water quality standards for discharge to municipal sewers" in China.

  14. Effect of chlorate, molybdate, and shikimic acid on Salmonella enterica serovar Typhimurium in aerobic and anaerobic cultures.

    Science.gov (United States)

    Oliver, Christy E; Beier, Ross C; Hume, Michael E; Horrocks, Shane M; Casey, Thomas A; Caton, Joel S; Nisbet, David J; Smith, David J; Krueger, Nathan A; Anderson, Robin C

    2010-04-01

    Experiments were conducted to determine factors that affect sensitivity of Salmonella enterica serovar Typhimurium to sodium chlorate (5mM). In our first experiment, cultures grown without chlorate grew more rapidly than those with chlorate. An extended lag before logarithmic growth was observed in anaerobic but not aerobic cultures containing chlorate. Chlorate inhibition of growth during aerobic culture began later than that observed in anaerobic cultures but persisted once inhibition was apparent. Conversely, anaerobic cultures appeared to adapt to chlorate after approximately 10h of incubation, exhibiting rapid compensatory growth. In anaerobic chlorate-containing cultures, 20% of total viable counts were resistant to chlorate by 6h and had propagated to 100% resistance (>10(9)CFU mL(-1)) by 24h. In the aerobic chlorate-containing cultures, 12.9% of colonies had detectable resistance to chlorate by 6h, but only 1% retained detectable resistance at 24h, likely because these cultures had opportunity to respire on oxygen and were thus not enriched via the selective pressure of chlorate. In another study, treatment with shikimic acid (0.34 mM), molybdate (1mM) or their combination had little effect on aerobic or anaerobic growth of Salmonella in the absence of added chlorate. As observed in our earlier study, chlorate resistance was not detected in any cultures without added chlorate. Chlorate resistant Salmonella were recovered at equivalent numbers regardless of treatment after 8h of aerobic or anaerobic culture with added chlorate; however, by 24h incubation chlorate sensitivity was completely restored to aerobic but not anaerobic cultures treated with shikimic acid or molybdate but not their combination. Results indicate that anaerobic adaptation of S. Typhimurium to sodium chlorate during pure culture is likely due to the selective propagation of low numbers of cells exhibiting spontaneous resistance to chlorate and this resistance is not reversible by

  15. Partition of aerobic and anaerobic swimming costs related to gait transitions in a labriform swimmer.

    Science.gov (United States)

    Svendsen, Jon C; Tudorache, Christian; Jordan, Anders D; Steffensen, John F; Aarestrup, Kim; Domenici, Paolo

    2010-07-01

    Members of the family Embiotocidae exhibit a distinct gait transition from exclusively pectoral fin oscillation to combined pectoral and caudal fin propulsion with increasing swimming speed. The pectoral-caudal gait transition occurs at a threshold speed termed U(p-c). The objective of this study was to partition aerobic and anaerobic swimming costs at speeds below and above the U(p-c) in the striped surfperch Embiotoca lateralis using swimming respirometry and video analysis to test the hypothesis that the gait transition marks the switch from aerobic to anaerobic power output. Exercise oxygen consumption rate was measured at 1.4, 1.9 and 2.3 L s(-1). The presence and magnitude of excess post-exercise oxygen consumption (EPOC) were evaluated after each swimming speed. The data demonstrated that 1.4 L s(-1) was below the U(p-c), whereas 1.9 and 2.3 L s(-1) were above the U(p-c). These last two swimming speeds included caudal fin propulsion in a mostly steady and unsteady (burst-assisted) mode, respectively. There was no evidence of EPOC after swimming at 1.4 and 1.9 L s(-1), indicating that the pectoral-caudal gait transition was not a threshold for anaerobic metabolism. At 2.3 L s(-1), E. lateralis switched to an unsteady burst and flap gait. This swimming speed resulted in EPOC, suggesting that anaerobic metabolism constituted 25% of the total costs. Burst activity correlated positively with the magnitude of the EPOC. Collectively, these data indicate that steady axial propulsion does not lead to EPOC whereas transition to burst-assisted swimming above U(p-c) is associated with anaerobic metabolism in this labriform swimmer.

  16. Aerobic and anaerobic profiles, heart rate and match analysis in older soccer players.

    Science.gov (United States)

    Tessitore, Antonio; Meeusen, Romain; Tiberi, Monica; Cortis, Cristina; Pagano, Rita; Capranica, Laura

    The aim of the present study was to provide aerobic (maximal oxygen consumption--VO2max) and anaerobic [jump tests: counter-movement jump (CMJ) and bounce jump (BJ); and running tests: 10 m sprint and 10 m dribbling the ball (10 mDB)] profiles of older soccer players (n = 12), and heart rate (HR), blood lactate accumulation (La) and match analysis (individual motor activity and team behaviour) aspects of a soccer match. The maximal aerobic test values were: HRmax, 154 +/- 14 beat min(-1); maximal power, 160 +/- 24 W; VO2peak, 36.3 +/- 11.3 ml kg(-1) min(-1); peak La, 8.2 +/- 1.8 mM. Jump test values were: CMJ, 21.2 +/- 4.5 cm; BJ, 17.3 +/- 4.8 cm. Running performances were: 10 m sprint, 2.06 +/- 0.36 s; 10 mDB, 2.17 +/- 0.21 s. Running showed similar values in the two halves (first half, 82%; second half, 88%), while walking lasted longer during the second half. Regarding the technical aspects of the match, 42% of the consecutive passes made in possession of the ball exceeded three passes, indicating that their play was structured in a cooperative manner. Older soccer players succeeded in maintaining good aerobic and anaerobic physical capability.

  17. Culturing aerobic and anaerobic bacteria and mammalian cells with a microfluidic differential oxygenator.

    Science.gov (United States)

    Lam, Raymond H W; Kim, Min-Cheol; Thorsen, Todd

    2009-07-15

    In this manuscript, we report on the culture of anaerobic and aerobic species within a disposable multilayer polydimethylsiloxane (PDMS) microfluidic device with an integrated differential oxygenator. A gas-filled microchannel network functioning as an oxygen-nitrogen mixer generates differential oxygen concentration. By controlling the relative flow rate of the oxygen and nitrogen input gases, the dissolved oxygen (DO) concentration in proximal microchannels filled with culture media are precisely regulated by molecular diffusion. Sensors consisting of an oxygen-sensitive dye embedded in the fluid channels permit dynamic fluorescence-based monitoring of the DO concentration using low-cost light-emitting diodes. To demonstrate the general utility of the platform for both aerobic and anaerobic culture, three bacteria with differential oxygen requirements (E. coli, A. viscosus, and F. nucleatum), as well as a model mammalian cell line (murine embryonic fibroblast cells (3T3)), were cultured. Growth characteristics of the selected species were analyzed as a function of eight discrete DO concentrations, ranging from 0 ppm (anaerobic) to 42 ppm (fully saturated).

  18. Removal of trichloroethylene (TCE) contaminated soil using a two-stage anaerobic-aerobic composting technique.

    Science.gov (United States)

    Ponza, Supat; Parkpian, Preeda; Polprasert, Chongrak; Shrestha, Rajendra P; Jugsujinda, Aroon

    2010-01-01

    The effect of organic carbon addition on remediation of trichloroethylene (TCE) contaminated clay soil was investigated using a two stage anaerobic-aerobic composting system. TCE removal rate and processes involved were determined. Uncontaminated clay soil was treated with composting materials (dried cow manure, rice husk and cane molasses) to represent carbon based treatments (5%, 10% and 20% OC). All treatments were spiked with TCE at 1,000 mg TCE/kg DW and incubated under anaerobic and mesophillic condition (35 degrees C) for 8 weeks followed by continuous aerobic condition for another 6 weeks. TCE dissipation, its metabolites and biogas composition were measured throughout the experimental period. Results show that TCE degradation depended upon the amount of organic carbon (OC) contained within the composting treatments/matrices. The highest TCE removal percentage (97%) and rate (75.06 micro Mole/kg DW/day) were obtained from a treatment of 10% OC composting matrices as compared to 87% and 27.75 micro Mole/kg DW/day for 20% OC, and 83% and 38.08 micro Mole/kg DW/day for soil control treatment. TCE removal rate was first order reaction kinetics. Highest degradation rate constant (k(1) = 0.035 day(- 1)) was also obtained from the 10% OC treatment, followed by 20% OC (k(1) = 0.026 day(- 1)) and 5% OC or soil control treatment (k(1) = 0.023 day(- 1)). The half-life was 20, 27 and 30 days, respectively. The overall results suggest that sequential two stages anaerobic-aerobic composting technique has potential for remediation of TCE in heavy texture soil, providing that easily biodegradable source of organic carbon is present.

  19. Characterization and treatment of Denizli landfill leachate using anaerobic hybrid/aerobic CSTR systems.

    Science.gov (United States)

    Ağdağ, Osman Nuri

    2011-01-01

    Leachate generated in municipal solid waste landfill contains large amounts of organic and inorganic contaminants. In the scope of the study, characterization and anaerobic/aerobic treatability of leachate from Denizli (Turkey) Sanitary Landfill were investigated. Time-based fluctuations in characteristics of leachate were monitored during a one-year period. In characterization study; chemical oxygen demand (COD), biochemical oxygen demand (BOD) dissolved oxygen, temperature, pH, alkalinity, volatile fatty acids, total nitrogen, NH4-N, BOD5/COD ratio, suspended solid, inert COD, anaerobic toxicity assay and heavy metals concentrations in leachate were monitored. Average COD, BOD and NH4-N concentration in leachate were measured as 18034 mg/l, 11504 mg/l and 454 mg/l, respectively. Generally, pollution parameters in leachate were higher in summer and relatively lower in winter due to dilution by precipitation. For treatment of leachate, two different reactors, namely anaerobic hybrid and aerobic completely stirred tank reactor (CSTR) having effective volumes of 17.7 and 10.5 litres, respectively, were used. After 41 days of start-up period, leachate was loaded to hybrid reactor at 10 different organic loading rates (OLRs). OLR was increased by increasing COD concentrations. COD removal efficiency of hybrid reactor was carried out at a maximum of 91%. A percentage of 96% of residual COD was removed in the aerobic reactor. NH4-N removal rate in CSTR was quite high. In addition, high methane content was obtained as 64% in the hybrid reactor. At the end of the study, after 170 operation days, it can be said that the hybrid reactor and CSTR were very effective for leachate treatment.

  20. Evaluation of integrated anaerobic-aerobic biofilm reactor for degradation of azo dye methyl orange.

    Science.gov (United States)

    Murali, V; Ong, Soon-An; Ho, Li-Ngee; Wong, Yee-Shian

    2013-09-01

    This study was to investigate the mineralization of wastewater containing methyl orange (MO) in integrated anaerobic-aerobic biofilm reactor with coconut fiber as bio-material. Different aeration periods (3h in phase 1 and 2; 3, 6 and 15 h in phase 3; 24 h in phase 4 and 5) in aerobic chamber were studied with different MO concentration 50, 100, 200, 200 and 300 mg/L as influent from phase 1-5. The color removals estimated from the standard curve of dye versus optical density at its maximum absorption wavelength were 97%, 96%, 97%, 97%, and 96% and COD removals were 75%, 72%, 63%, 81%, and 73% in phase 1-5, respectively. The MO decolorization and COD degradation followed first-order kinetic model and second-order kinetic model, respectively. GC-MS analysis indicated the symmetrical cleavage of azo bond and the reduction in aromatic peak ensured the partial mineralization of MO.

  1. Trace metal pyritization variability in response to mangrove soil aerobic and anaerobic oxidation processes.

    Science.gov (United States)

    Machado, W; Borrelli, N L; Ferreira, T O; Marques, A G B; Osterrieth, M; Guizan, C

    2014-02-15

    The degree of iron pyritization (DOP) and degree of trace metal pyritization (DTMP) were evaluated in mangrove soil profiles from an estuarine area located in Rio de Janeiro (SE Brazil). The soil pH was negatively correlated with redox potential (Eh) and positively correlated with DOP and DTMP of some elements (Mn, Cu and Pb), suggesting that pyrite oxidation generated acidity and can affect the importance of pyrite as a trace metal-binding phase, mainly in response to spatial variability in tidal flooding. Besides these aerobic oxidation effects, results from a sequential extraction analyses of reactive phases evidenced that Mn oxidized phase consumption in reaction with pyrite can be also important to determine the pyritization of trace elements. Cumulative effects of these aerobic and anaerobic oxidation processes were evidenced as factors affecting the capacity of mangrove soils to act as a sink for trace metals through pyritization processes.

  2. Sequential anaerobic-aerobic biological treatment of colored wastewaters: case study of a textile dyeing factory wastewater.

    Science.gov (United States)

    Abiri, Fardin; Fallah, Narges; Bonakdarpour, Babak

    2017-03-01

    In the present study the feasibility of the use of a bacterial batch sequential anaerobic-aerobic process, in which activated sludge was used in both parts of the process, for pretreatment of wastewater generated by a textile dyeing factory has been considered. Activated sludge used in the process was obtained from a municipal wastewater treatment plant and adapted to real dyeing wastewater using either an anaerobic-only or an anaerobic-aerobic process over a period of 90 days. The use of activated sludge adapted using the anaerobic-aerobic process resulted in a higher overall decolorization efficiency compared to that achieved with activated sludge adapted using the anaerobic-only cycles. Anaerobic and aerobic periods of around 34 and 22 hours respectively resulted in an effluent with chemical oxygen demand (COD) and color content which met the standards for discharge into the centralized wastewater treatment plant of the industrial estate in which the dyeing factory was situated. Neutralization of the real dyeing wastewater and addition of carbon source to it, both of which results in significant increase in the cost of the bacterial treatment process, was not found to be necessary to achieve the required discharge standards.

  3. Repeated sprint performance and metabolic recovery curves: effects of aerobic and anaerobic characteristics.

    Science.gov (United States)

    de Aguiar, Rafael Alves; Turnes, Tiago; Santos de Oliveira Cruz, Rogério; Salvador, Amadeo Félix; Caputo, Fabrizio

    2015-05-01

    To examine the influence of aerobic and anaerobic indices on repeated sprint (RS) performance and ability (RSA), 8 sprinters (SPR), 8 endurance runners (END), and 8 active participants (ACT) performed the following tests: (i) incremental test; (ii) 1-min test to determine first decay time constant of pulmonary oxygen uptake off-kinetics and parameters related to anaerobic energy supply, lactate exchange, and removal abilities from blood lactate kinetics; and (iii) RS test (ten 35-m sprints, departing every 20 s) to determine best (RSbest) and mean (RSmean) sprint times and percentage of sprint decrement (%Dec). While SPR had a 98%-100% likelihood of having the fastest RSbest (Cohen's d of 1.8 and 1.4 for ACT and END, respectively) and RSmean (2.1 and 0.9 for ACT and END, respectively), END presented a 97%-100% likelihood of having the lowest %Dec (0.9 and 2.2 for ACT and SPR, respectively). RSmean was very largely correlated with RSbest (r=0.85) and moderately correlated with estimates of anaerobic energy supply (r=-0.40 to -0.49). RSmean adjusted for RSbest (which indirectly reflects RSA) was largely correlated with lactate exchange ability (r=0.55). Our results confirm the importance of locomotor- and anaerobic-related variables to RS performance, and highlight the importance of disposal of selected metabolic by-products to RSA.

  4. Microbial decolorization of reactive black-5 in a two-stage anaerobic-aerobic reactor using acclimatized activated textile sludge.

    Science.gov (United States)

    Mohanty, Sagarika; Dafale, Nishant; Rao, Nageswara Neti

    2006-10-01

    A two-stage anaerobic-aerobic treatment process based on mixed culture of bacteria isolated from textile dye effluent was used to degrade reactive black 5 dye (RB-5). The anaerobic step was studied in more detail by varying the dye concentration from 100 to 3000 mg l(-1). The results showed that major decolorization was achieved during the anaerobic process. The time required for decolorization by > 90% increased as the concentration of the dye increased. It was also found that maintaining dissolved oxygen (DO) concentration below 0.5 mg l(-1 )and addition of a co-substrate viz., glucose, facilitates anaerobic decolorization reaction remarkably. An attempt was made to identify the metabolites formed in anaerobic process by using high performance liquid chromatography (HPLC) and UV-VIS spectrophotometry. A plate assay was performed for the detection of dominant decolorizing bacteria. Only a few bacterial colonies with high clearing zones (decolorization zones) were found. The results showed that under anaerobic condition RB-5 molecules were reduced and aromatic amines were generated. The aromatic amine metabolite was partly removed in subsequent aerobic bio-treatment. It was possible to achieve more than 90% decolorization and approximately 46% reduction in amine metabolite concentration through two-stage anaerobic-aerobic treatment after a reaction period of 2 days.

  5. Comprehensive microbial analysis of combined mesophilic anaerobic-thermophilic aerobic process treating high-strength food wastewater

    DEFF Research Database (Denmark)

    Jang, Hyun Min; Ha, Jeong Hyub; Park, Jong Moon

    2015-01-01

    A combined mesophilic anaerobic-thermophilic aerobic process was used to treat high-strength food wastewater in this study. During the experimental period, most of solid residue from the mesophilic anaerobic reactor (R1) was separated by centrifugation and introduced into the thermophilic aerobic...... reactor (R2) for further digestion. Then, thermophilic aerobically-digested sludge was reintroduced into R1 to enhance reactor performance. The combined process was operated with two different Runs: Run I with hydraulic retention time (HRT)=40d (corresponding OLR=3.5kgCOD/m3 d) and Run II with HRT=20d...... (corresponding OLR=7kgCOD/m3). For a comparison, a single-stage mesophilic anaerobic reactor (R3) was operated concurrently with same OLRs and HRTs as the combined process. During the overall digestion, all reactors showed high stability without pH control. The combined process demonstrated significantly higher...

  6. Biodegradation kinetics of selected brominated flame retardants in aerobic and anaerobic soil

    Energy Technology Data Exchange (ETDEWEB)

    Nyholm, Jenny Rattfelt, E-mail: jenny.rattfelt@chem.umu.s [Department of Chemistry, Umea University, SE-901 87 Umea (Sweden); Lundberg, Charlott; Andersson, Patrik L. [Department of Chemistry, Umea University, SE-901 87 Umea (Sweden)

    2010-06-15

    The purpose of the present study was to investigate the biodegradation kinetics in aerobic and anaerobic soil of the following brominated flame retardants: 2,4,4'-tribromodiphenyl ether (BDE 28), decabromodiphenyl ether (BDE 209), tetrabromobisphenol A (TBBPA), 1,2-dibromo-4-(1,2-dibromoethyl)cyclohexane (TBECH), 2,4,6-tribromophenol (246BrPh), and hexabromobenzene (HxBrBz). For comparison, the biodegradation of the chlorinated compounds 2,4,4'-trichlorodiphenyl ether (CDE 28), 2,4,6-trichlorophenol (246ClPh), hexachlorobenzene (HxClBz), and 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153) was also assessed. In aerobic soil, BDE 209 showed no significant degradation during the test period, but concentrations of the other BFRs declined, with half-lives decreasing in the following order: BDE 28 > TBBPA > TBECH > HxBrBz > 246BrPh. Declines in almost the same order were observed in anaerobic soil: BDE 28, BDE 209 > TBBPA > HxBrBz > TBECH >246BrPh. - Intra- and extrapolated half-lives in soil of tested brominated flame retardants ranged from 7 days for 2,4,6-tribromorophenol to >400 days for decabromodiphenyl ether.

  7. Development of microorganisms during storage of wet brewery waste under aerobic and anaerobic conditions

    Directory of Open Access Journals (Sweden)

    Leiliane Cristine de Souza

    2012-01-01

    Full Text Available This research study was conducted to evaluate, by means of microbiological analyses, the preservation of wet brewery waste stored under aerobic and anaerobic conditions, regarding the development of filamentous fungi, yeasts and lactic-acid bacteria. The following treatments were used: untreated brewery waste, pre-dried brewery waste silage, brewery waste silage with effluent drainage and BW silage without effluent drainage. Silos made of PVC and equipped with Bunsen valves to allow gases to escape were used. Dry matter (DM, crude protein (CP, temperature (°C and pH in the untreated BW, in the stored brewery waste and in the brewery waste silage upon silo opening, after 60 days of ensilage were analyzed. A completely randomized design was used. The data were subjected to analysis of variance, and the means were compared by the Tukey test at the 5% probability level. The preservation of brewery waste packaged under aerobic conditions was not appropriate due to the development of filamentous fungi and yeasts; however, storage under anaerobic conditions proved to be an effective conservation process.

  8. Biodegradation of azo dyes in a sequential anaerobic-aerobic system.

    Science.gov (United States)

    Rajaguru, P; Kalaiselvi, K; Palanivel, M; Subburam, V

    2000-08-01

    A sequential anaerobic aerobic treatment process based on mixed culture of bacteria isolated from textile dye effluent-contaminated soil was used to degrade sulfonated azo dyes Orange G (OG), Amido black 10B (AB), Direct red 4BS (DR) and Congo red (CR). Under anaerobic conditions in a fixed-bed column using glucose as co-substrate, the azo dyes were reduced and amines were released by the bacterial biomass. The amines were completely mineralized in a subsequent aerobic treatment using the same isolates. The maximum degradation rate observed in the treatment system for OG was 60.9 mg/l per day (16.99 mg/g glucose utilized), for AB 571.3 mg/l per day (14.46 mg/g glucose utilized), for DR 112.5 mg/l per day (32.02 mg/g glucose utilized) and for CR 134.9 mg/l per day (38.9 mg/g glucose utilized).

  9. Kinetics of the biodegradation of green table olive wastewaters by aerobic and anaerobic treatments

    Energy Technology Data Exchange (ETDEWEB)

    Beltran, J. [Departamento de Ingenieria Quimica y Energetica, Universidad de Extremadura, 06071 Badajoz (Spain)], E-mail: jbelther@unex.es; Gonzalez, T.; Garcia, J. [Departamento de Ingenieria Quimica y Energetica, Universidad de Extremadura, 06071 Badajoz (Spain)

    2008-06-15

    The biodegradation of the organic pollutant matter present in green table olive wastewater (GTOW) is studied in batch reactors by an aerobic biodegradation and by an anaerobic digestion. In the aerobic biodegradation, the evolution of the substrate (in terms of chemical and biochemical oxygen demand), biomass, and total polyphenolic compounds present in the wastewater are followed during the process, and a kinetic study is performed using Contois' model, which when applied to the experimental results provides the kinetic parameter of this model, resulting in a modified Contois' equation (q = 3.3S/(0.31S{sub 0}X + X), gCOD/gVSS d{sup -1}). Other kinetic parameters were determined: the cellular yield coefficient (Y{sub X/S} = 5.7 x 10{sup -2} gVSS/gCOD) and the kinetic constant of cellular death phase (k{sub d} = 0.16 d{sup -1}). Similarly, in the anaerobic digestion, the evolution of the substrate digested and the methane produced are followed, and the kinetic study is conducted using a modified Monod model combined with the Levenspiel model, due to the presence of inhibition effects. This model leads to the determination of the kinetic parameters: kinetic constant when no inhibitory substance is present (k{sub M0} = 8.4 x 10{sup -2} h{sup -1}), critical substrate concentration of inhibition (TP* = 0.34 g/L) and inhibitory parameter (n = 2.25)

  10. Kinetics of the biodegradation of green table olive wastewaters by aerobic and anaerobic treatments.

    Science.gov (United States)

    Beltran, J; Gonzalez, T; Garcia, J

    2008-06-15

    The biodegradation of the organic pollutant matter present in green table olive wastewater (GTOW) is studied in batch reactors by an aerobic biodegradation and by an anaerobic digestion. In the aerobic biodegradation, the evolution of the substrate (in terms of chemical and biochemical oxygen demand), biomass, and total polyphenolic compounds present in the wastewater are followed during the process, and a kinetic study is performed using Contois' model, which when applied to the experimental results provides the kinetic parameter of this model, resulting in a modified Contois' equation (q=3.3S/(0.31S(0)X+X), gCOD/gVSS d(-1)). Other kinetic parameters were determined: the cellular yield coefficient (YX/S=5.7x10(-2) gVSS/gCOD) and the kinetic constant of cellular death phase (kd=0.16 d(-1)). Similarly, in the anaerobic digestion, the evolution of the substrate digested and the methane produced are followed, and the kinetic study is conducted using a modified Monod model combined with the Levenspiel model, due to the presence of inhibition effects. This model leads to the determination of the kinetic parameters: kinetic constant when no inhibitory substance is present (kM0=8.4x10(-2) h(-1)), critical substrate concentration of inhibition (TP*=0.34 g/L) and inhibitory parameter (n=2.25).

  11. Impact of aerobic and anaerobic exercise training on oxidative stress and antioxidant defense in athletes.

    Science.gov (United States)

    Park, Song-Young; Kwak, Yi-Sub

    2016-04-01

    Exercise mediates an excessive free radical production leading to oxidative stress (OS). The body has natural antioxidant systems that help decrease OS, and these systems may be enhanced with exercise training. However, only a few studies have investigated the differences in resting OS and antioxidant capacity (AOC) between aerobically trained athletes (ET), anaerobically trained athletes (RT), and untrained individuals (UT). Therefore, this study sought to investigate the resting and postexercise OS and AOC in ET, RT, and UT. Sixty healthy young males (26.6±0.8 yr) participated in this study. Subjects were divided into three groups, ET, RT, and UT by distinct training background. Resting plasma malondialdehyde (MDA) and protein carbonyls (PC) were not significantly different in ET, RT, and UT. However, MDA and PC were significantly increased following a graded exercise test (GXT) in UT but not in ET and RT. Resting total antioxidant capacity (TAC) levels and TAC were not different in ET, RT, and UT. Interestingly, TAC levels significantly decreased after the GXT in all groups. Additionally, UT showed lower post-exercise TAC levels compared to ET and RT. These results showed that ET, RT, and UT have similar OS and AOC at rest. However, both ET and RT have greater AOC against exercise mediated OS compared to UT. These findings may explain, at least in part, why both aerobic and anaerobic types of exercise training improve redox balance. However, it appears there is no specific exercise type effect in terms of redox balance.

  12. Reductive transformation and detoxification mechanism of 2,4-dinitrochlorobenzene in combined zero valent iron and anaerobic-aerobic process

    Institute of Scientific and Technical Information of China (English)

    Jinyou Shen; Zongyuan Zhou; Changjin Ou; Xiuyun Sun; Jiansheng Li; Weiqing Han; Lin Zhou; Lianjun Wang

    2012-01-01

    A combined zero valent iron (ZVI) and anaerobic-aerobic process was adopted for the treatment of 2,4-dinitrochlorobenzene (DNCB)-containing wastewater.The transformation pathway,reduction of acute toxicity and enhancement of biodegradability were investigated.After pretreatment by ZVI,DNCB in wastewater could be completely converted into 2,4-diaminochlorobenzene (DACB).The ratio of BOD5/COD increased from 0.005 ± 0.001 to 0.168 ± 0.007,while EC50,48 hr (V/V) increased from 0.65% to 5.20%,indicating the enhancement of biodegradability and reduction of acute toxicity with the pretreatment by ZVI.DACB was further dechlorinated to m-phenylenediamine during the anaerobic process using methanol as electron donor,with EC50,48 hr increasing from 5.20% to 48.2%.After the subsequent anaerobic-aerobic process,m-phenylenediamine was degraded completely,with effluent COD of 67.5 ± 10.8 mg/L.This effluent of the subsequent anaerobic-aerobic process was not toxic to zebrafish.The combined ZVI and anaerobic-aerobic process offers bright prospects for the treatment of chlorinated nitroaromatic compound-containing wastewater.

  13. Maximal aerobic and anaerobic power generation in large crocodiles versus mammals: implications for dinosaur gigantothermy.

    Directory of Open Access Journals (Sweden)

    Roger S Seymour

    Full Text Available Inertial homeothermy, the maintenance of a relatively constant body temperature that occurs simply because of large size, is often applied to large dinosaurs. Moreover, biophysical modelling and actual measurements show that large crocodiles can behaviourally achieve body temperatures above 30°C. Therefore it is possible that some dinosaurs could achieve high and stable body temperatures without the high energy cost of typical endotherms. However it is not known whether an ectothermic dinosaur could produce the equivalent amount of muscular power as an endothermic one. To address this question, this study analyses maximal power output from measured aerobic and anaerobic metabolism in burst exercising estuarine crocodiles, Crocodylusporosus, weighing up to 200 kg. These results are compared with similar data from endothermic mammals. A 1 kg crocodile at 30°C produces about 16 watts from aerobic and anaerobic energy sources during the first 10% of exhaustive activity, which is 57% of that expected for a similarly sized mammal. A 200 kg crocodile produces about 400 watts, or only 14% of that for a mammal. Phosphocreatine is a minor energy source, used only in the first seconds of exercise and of similar concentrations in reptiles and mammals. Ectothermic crocodiles lack not only the absolute power for exercise, but also the endurance, that are evident in endothermic mammals. Despite the ability to achieve high and fairly constant body temperatures, therefore, large, ectothermic, crocodile-like dinosaurs would have been competitively inferior to endothermic, mammal-like dinosaurs with high aerobic power. Endothermy in dinosaurs is likely to explain their dominance over mammals in terrestrial ecosystems throughout the Mesozoic.

  14. Maximal aerobic and anaerobic power generation in large crocodiles versus mammals: implications for dinosaur gigantothermy.

    Science.gov (United States)

    Seymour, Roger S

    2013-01-01

    Inertial homeothermy, the maintenance of a relatively constant body temperature that occurs simply because of large size, is often applied to large dinosaurs. Moreover, biophysical modelling and actual measurements show that large crocodiles can behaviourally achieve body temperatures above 30°C. Therefore it is possible that some dinosaurs could achieve high and stable body temperatures without the high energy cost of typical endotherms. However it is not known whether an ectothermic dinosaur could produce the equivalent amount of muscular power as an endothermic one. To address this question, this study analyses maximal power output from measured aerobic and anaerobic metabolism in burst exercising estuarine crocodiles, Crocodylusporosus, weighing up to 200 kg. These results are compared with similar data from endothermic mammals. A 1 kg crocodile at 30°C produces about 16 watts from aerobic and anaerobic energy sources during the first 10% of exhaustive activity, which is 57% of that expected for a similarly sized mammal. A 200 kg crocodile produces about 400 watts, or only 14% of that for a mammal. Phosphocreatine is a minor energy source, used only in the first seconds of exercise and of similar concentrations in reptiles and mammals. Ectothermic crocodiles lack not only the absolute power for exercise, but also the endurance, that are evident in endothermic mammals. Despite the ability to achieve high and fairly constant body temperatures, therefore, large, ectothermic, crocodile-like dinosaurs would have been competitively inferior to endothermic, mammal-like dinosaurs with high aerobic power. Endothermy in dinosaurs is likely to explain their dominance over mammals in terrestrial ecosystems throughout the Mesozoic.

  15. Enhanced phosphorus recovery and biofilm microbial community changes in an alternating anaerobic/aerobic biofilter.

    Science.gov (United States)

    Tian, Qing; Ong, Say Kee; Xie, Xuehui; Li, Fang; Zhu, Yanbin; Wang, Feng Rui; Yang, Bo

    2016-02-01

    The operation of an alternating anaerobic/aerobic biofilter (AABF), treating synthetic wastewater, was modified to enhance recovery of phosphorus (P). The AABF was periodically fed with an additional carbon source during the anaerobic phase to force the release of biofilm-sequestered P which was then harvested and recovered. A maximum of 48% of the total influent P was found to be released in the solution for recovery. Upon implementation of periodic P bio-sequestering and P harvesting, the predominant bacterial communities changed from β-Proteobacteria to γ-Proteobacteria groups. The genus Pseudomonas of γ-Proteobacteria was found to enrich greatly with 98% dominance. Dense intracellular poly-P granules were found within the cells of the biofilm, confirming the presence of P accumulating organisms (PAOs). Periodic addition of a carbon source to the AABF coupled with intracellular P reduction during the anaerobic phase most probably exerted environmental stress in the selection of Pseudomonas PAOs over PAOs of other phylogenic types. Results of the study provided operational information on the selection of certain microbial communities for P removal and recovery. This information can be used to further advance P recovery in biofilm systems such as the AABFs.

  16. Impact of intermittent aerations on leachate quality and greenhouse gas reduction in the aerobic-anaerobic landfill method.

    Science.gov (United States)

    Nag, Mitali; Shimaoka, Takayuki; Komiya, Teppei

    2016-09-01

    The aerobic-anaerobic landfill method (AALM) is a novel approach in solid waste management that could shorten the landfill post-closure period and minimize the environmental loads. In this study, the aerobic-anaerobic landfill method was evaluated by using intermittent aeration. In addition, the nitrification-denitrification process was assessed as a means of reducing the emission of greenhouse gases (GHGs) and improving the leachate quality during the degradation of the organic solid waste. The leachate quality and the gas composition in each of the reactors were measured during the experimental period (408days). The aeration process entailed the injection of air into plexiglass cylinders (200cm height×10 cm diameter), filled with fresh organic solid waste collected from a composting plant. Different aeration routines were applied, namely, continuous aeration (aerobic reactor A), aeration for three days/week (aerobic-anaerobic reactor B), aeration for 6h/day (aerobic-anaerobic reactor C), and no aeration (non-aerated reactor D). It was found that aerobic reactor A produced the best results in terms of reduction of GHGs and improvement of the leachate quality. The aerobic-anaerobic reactor C was found to be more effective than reactor B in respect of both the emission of GHGs and the leachate quality; moreover, compared with aerobic reactor A, energy costs were reduced by operating this reactor. The transition period phenomenon was investigated during an intensive seven-day experiment conducted on the discharged leachate obtained from aerobic-anaerobic reactors B and C. The experiment concerned the differences in the composition of the gas during the aeration and the non-aeration periods. It was found that the transition period between the aeration and non-aeration cycles, which followed the simultaneous nitrification-denitrification had a considerable effect on the leachate quality of both the reactors. The results indicated that AALM has the potential to reduce

  17. Anaerobic-aerobic small scale on-site wastewater treatment process. Kenkiter dot koki shori hoshiki jokaso

    Energy Technology Data Exchange (ETDEWEB)

    Inamori, Y. (National Institute for Environmental Studies, Tsukuba (Japan))

    1991-01-05

    As a part of the developmental project of advanced domestic wastewater treatment facilities, the following three types of small scale on-site anaerobic-aerobic wastewater treatment processes with removal functions of both BOD and nutritive salts were developed with targets of 10mg/l in BOD, 10mg/l in T-N and 1mg/l or less in T-P: an anaerobic-aerobic bio-filter treatment process with the adjustment function of hydraulic loading (A), an anaerobic-aerobic immobilized microorganisms packed fluidized bed-biofilm process (B) and a circulated anaerobic-aerobic fixed bed process (C). As the results of demonstration tests of each process using real wastewater, each process could achieve nearly the desired end, and the A process was superior in removal functions of organic substances, nitrogen and phosphorus. The C process was superior in stable removal functions of BOD and nitrogen as well as its easy production and low cost. 7 refs., 7 figs., 1 tab.

  18. Activity, Microenvironments, and Community Structure of Aerobic and Anaerobic Ammonium Oxidizing Prokaryotes in Estuarine Sediment (Randers Fjord, DK)

    DEFF Research Database (Denmark)

    Schramm, Andreas; Revsbech, Niels Peter; Dalsgaard, Tage

    2006-01-01

    ACTIVITY, MICROENVIRONMENTS, AND COMMUNITY STRUCTURE OF AEROBIC AND ANAEROBIC AMMONIUM OXIDIZING PROKARYOTES IN ESTUARINE SEDIMENT (RANDERS FJORD, DK) A. Schramm 1, N.P. Revsbech 1, T. Dalsgaard 2, E. Piña-Ochoa 3, J. de la Torré 4, D.A. Stahl 4, N. Risgaard-Petersen 2 1 Department of Biological...

  19. Effects of Short Term Camp Periods on Aerobic and Anaerobic Performance Parameters in Ice Hockey National Team Athletes

    Science.gov (United States)

    Eler, Serdar

    2016-01-01

    This study was conducted for determining the effects of trainings, applied to athletes during short term camp period, on their aerobic and anaerobic performance. Measurements were made by the participation of 28 volunteer male ice hockey national team players. During the 15-day camp period, 10-minute running and stretching for warming and then…

  20. Relationship Between Repeated Sprint Performance and both Aerobic and Anaerobic Fitness

    Science.gov (United States)

    Dardouri, Wajdi; Selmi, Mohamed Amin; Sassi, Radhouane Haj; Gharbi, Zied; Rebhi, Ahmed; Yahmed, Mohamed Haj; Moalla, Wassim

    2014-01-01

    The aims of this study were firstly, to examine the relationship between repeated sprint performance indices and anaerobic speed reserve (AnSR), aerobic fitness and anaerobic power and secondly, to identify the best predictors of sprinting ability among these parameters. Twenty nine subjects (age: 22.5 ± 1.6 years, body height: 1.8 ± 0.1 m, body mass: 68.8 ± 8.5 kg, body mass index (BMI): 22.2 ± 2.1 kg•m-2, fat mass: 11.3 ± 2.9 %) participated in this study. All participants performed a 30 m sprint test (T30) from which we calculated the maximal anaerobic speed (MAnS), vertical and horizontal jumps, 20m multi-stage shuttle run test (MSRT) and repeated sprint test (10 × 15 m shuttle run). AnSR was calculated as the difference between MAnS and the maximal speed reached in the MSRT. Blood lactate sampling was performed 3 min after the RSA protocol. There was no significant correlation between repeated sprint indices (total time (TT); peak time (PT), fatigue index (FI)) and both estimated VO2max and vertical jump performance). TT and PT were significantly correlated with T30 (r=0.63, p=0.001 and r=0.62, p=0.001; respectively), horizontal jump performance (r = −0.47, p = 0.001 and r = −0.49, p = 0.006; respectively) and AnSR (r=−0.68, p= 0.001 and r=−0.70, p=0.001, respectively). Significant correlations were found between blood lactate concentration and TT, PT, and AnSR (r=−0.44, p=0.017; r=−0.43, p=0.018 and r=0.44, p=0.016; respectively). Stepwise multiple regression analyses demonstrated that AnSR was the only significant predictor of the TT and PT, explaining 47% and 50% of the shared variance, respectively. Our findings are of particular interest for coaches and fitness trainers in order to predict repeated sprint performance by using AnSR that can easily identify the respective upper performance limits supported by aerobic and anaerobic power of a player involved in multi-sprint team sports. PMID:25031682

  1. Relationship Between Repeated Sprint Performance and both Aerobic and Anaerobic Fitness.

    Science.gov (United States)

    Dardouri, Wajdi; Selmi, Mohamed Amin; Sassi, Radhouane Haj; Gharbi, Zied; Rebhi, Ahmed; Yahmed, Mohamed Haj; Moalla, Wassim

    2014-03-27

    The aims of this study were firstly, to examine the relationship between repeated sprint performance indices and anaerobic speed reserve (AnSR), aerobic fitness and anaerobic power and secondly, to identify the best predictors of sprinting ability among these parameters. Twenty nine subjects (age: 22.5 ± 1.6 years, body height: 1.8 ± 0.1 m, body mass: 68.8 ± 8.5 kg, body mass index (BMI): 22.2 ± 2.1 kg•m-2, fat mass: 11.3 ± 2.9 %) participated in this study. All participants performed a 30 m sprint test (T30) from which we calculated the maximal anaerobic speed (MAnS), vertical and horizontal jumps, 20m multi-stage shuttle run test (MSRT) and repeated sprint test (10 × 15 m shuttle run). AnSR was calculated as the difference between MAnS and the maximal speed reached in the MSRT. Blood lactate sampling was performed 3 min after the RSA protocol. There was no significant correlation between repeated sprint indices (total time (TT); peak time (PT), fatigue index (FI)) and both estimated VO2max and vertical jump performance). TT and PT were significantly correlated with T30 (r=0.63, p=0.001 and r=0.62, p=0.001; respectively), horizontal jump performance (r = -0.47, p = 0.001 and r = -0.49, p = 0.006; respectively) and AnSR (r=-0.68, p= 0.001 and r=-0.70, p=0.001, respectively). Significant correlations were found between blood lactate concentration and TT, PT, and AnSR (r=-0.44, p=0.017; r=-0.43, p=0.018 and r=0.44, p=0.016; respectively). Stepwise multiple regression analyses demonstrated that AnSR was the only significant predictor of the TT and PT, explaining 47% and 50% of the shared variance, respectively. Our findings are of particular interest for coaches and fitness trainers in order to predict repeated sprint performance by using AnSR that can easily identify the respective upper performance limits supported by aerobic and anaerobic power of a player involved in multi-sprint team sports.

  2. Effects of permafrost thaw on carbon emissions under aerobic and anaerobic environments in the Great Hing'an Mountains, China.

    Science.gov (United States)

    Song, Changchun; Wang, Xianwei; Miao, Yuqing; Wang, Jiaoyue; Mao, Rong; Song, Yanyu

    2014-07-15

    The carbon (C) pool of permafrost peatland is very important for the global C cycle. Little is known about how permafrost thaw could influence C emissions in the Great Hing'an Mountains of China. Through aerobic and anaerobic incubation experiments, we studied the effects of permafrost thaw on CH4 and CO2 emissions. The rates of CH4 and CO2 emissions were measured at -10, 0 and 10°C. Although there were still C emissions below 0°C, rates of CH4 and CO2 emissions significantly increased with permafrost thaw under aerobic and anaerobic conditions. The C release under aerobic conditions was greater than under anaerobic conditions, suggesting that permafrost thaw and resulting soil environment change should be important influences on C emissions. However, CH4 stored in permafrost soils could affect accurate estimation of CH4 emissions from microbial degradation. Calculated Q10 values in the permafrost soils were significantly higher than values in active-layer soils under aerobic conditions. Our results highlight that permafrost soils have greater potential decomposability than soils of the active layer, and such carbon decomposition would be more responsive to the aerobic environment.

  3. Treatment of phenolics, aromatic hydrocarbons, and cyanide-bearing wastewater in individual and combined anaerobic, aerobic, and anoxic bioreactors.

    Science.gov (United States)

    Sharma, Naresh K; Philip, Ligy

    2015-01-01

    Studies were conducted on a mixture of pollutants commonly found in coke oven wastewater (CWW) to evaluate the biodegradation of various pollutants under anaerobic, aerobic, and anoxic conditions. The removal of the pollutants was monitored during individual bioreactor operation and using a combination of bioreactors operating in anaerobic-aerobic-anoxic sequence. While studying the performance of individual reactors, it was observed that cyanide removal (83.3 %) was predominant in the aerobic bioreactor, while much of the chemical oxygen demand (COD) (69 %) was consumed in the anoxic bioreactor. With the addition of cyanide, the COD removal efficiency was affected in all the bioreactors, and several intermediates were detected. While treating synthetic CWW using the combined bioreactor system, the overall COD removal efficiency was 86.79 % at an OLR of 2.4 g COD/L/day and an HRT of 96 h. The removal efficiency of 3,5-xylenol and cyanide, with inlet concentration of 150 and 10 mg/L, was found to be 91.8 and 93.6 % respectively. It was found that the impact of xylenol on the performance of the bioreactors was less than cyanide toxicity. Molecular analysis using T-RFLP revealed the dominance of strictly aerobic, mesophilic proteobacterium, Bosea minatitlanensis, in the aerobic bioreactor. The anoxic bioreactor was dominant with Rhodococcus pyridinivorans, known for its remarkable aromatic decomposing activity, while an unclassified Myxococcales bacterium was identified as the predominant bacterial species in the anaerobic bioreactor.

  4. Aerobic and anaerobic work capacities and leg muscle characteristics in elite orienteers.

    Science.gov (United States)

    Rolf, C; Andersson, G; Westblad, P; Saltin, B

    1997-02-01

    Aerobic and anaerobic work capacities, leg muscle structure and metabolic characteristics of m. vastus lateralis (NT), m. rectus femoris (RG) and mm. gastrocnemii (NT and RG) were analysed in five male and seven female elite orienteers from the Swedish National team (NT) and a reference group (RG) of eight male and 10 female upcoming orienteers, all in optimal shape at the end of a competitive season. Maximal oxygen uptake was 78.4 ml/kg/min for NT men (range 75-81) and 67.8 ml/kg/min for NT women (range 62-71), for both groups significantly higher (P orienteering. It remains to be shown how these laboratory data are related to individual performance in authentic orienteering competitions.

  5. Primary biodegradation of veterinary antibiotics in aerobic and anaerobic surface water simulation systems

    DEFF Research Database (Denmark)

    Ingerslev, Flemming; Toräng, Lars; Loke, M.-L.

    2001-01-01

    The primary aerobic and anaerobic biodegradability at intermediate concentrations (50-5000 mug/l) of the antibiotics olaquindox (OLA), metronidazole (MET), tylosin (TYL) and oxytetracycline (OTC) was studied in a simple shake flask system simulating the conditions in surface waters. The purpose...... of the study was to provide rate data for primary biodegradation in the scenario where antibiotics pollute surface waters as a result of run-off from arable land. The source of antibiotics may be application of manure as fertilizer or excreta of grazing animals. Assuming first-order degradation kinetics...... substances. The biodegradation behaviour was influenced by neither the concentrations of antibiotics nor the time of the year and location for sampling of surface water. Addition of 1 g/l of sediment or 3 mg/l of activated sludge from wastewater treatment increased the biodegradation potential which...

  6. Microbiological corrosion in aerobic and anaerobic waste purification plants: safety and efficiency problems

    Energy Technology Data Exchange (ETDEWEB)

    Perego, P. [ISTIC - Institute of Chemical and Process Engineering ``G.B. Bonino``, University of Genoa, Via Opera Pia 15-16145 Genoa (Italy); Fabiano, B. [ISTIC - Institute of Chemical and Process Engineering ``G.B. Bonino``, University of Genoa, Via Opera Pia 15-16145 Genoa (Italy); Pastorino, R. [ISTIC - Institute of Chemical and Process Engineering ``G.B. Bonino``, University of Genoa, Via Opera Pia 15-16145 Genoa (Italy); Randi, G. [ISTIC - Institute of Chemical and Process Engineering ``G.B. Bonino``, University of Genoa, Via Opera Pia 15-16145 Genoa (Italy)

    1997-07-01

    Carbon steels and stainless steels are largely employed in order to build up biological purification systems of urban and industrial waste. The former are applied in building up tanks, vessels and pipes; the latter are employed in building up operator machines and appliances. This paper presents a study of the corrosion behaviour of these materials in anaerobic and aerobic environments. Tests have been carried out by continuous immersion in a biologic liquid with mixed flora. The observation of samples taken at different times of exposure has given information of great interest of application, especially about the mechanisms of localized corrosion phenomena and about the behaviour of these materials in function. Besides the presented study shows original possibilities for the assessment of the corrosion phenomenon that can be adopted and developed further still. (orig.). With 7 figs., 4 tabs.

  7. Monitoring of growth and physiological activities of biofilm during succession on polystyrene from activated sludge under aerobic and anaerobic conditions.

    Science.gov (United States)

    Naz, Iffat; Batool, Syeda Ain-ul; Ali, Naeem; Khatoon, Nazia; Atiq, Niama; Hameed, Abdul; Ahmed, Safia

    2013-08-01

    The present research work monitored the successive biofilm development and its catabolic role in the degradation of polystyrene (PS). PS material was artificially colonized with biofilm by incubating it with activated sludge under aerobic and anaerobic conditions. Biofilm formation was monitored by gravimetric weight analysis, spectrophotometric absorbance technique, heterotrophic plate count, and scanning electron microscopy under aerobic and anaerobic conditions. The wet weight (1.59 and 1.17 g) and dry weight (0.41 and 0.08 g) of a biofilm showed a significant constant increase under aerobic and anaerobic conditions, respectively, from first till 9 weeks of incubation. Plate count of the selected bacteria (Escherichia coli, Salmonella typhimurium, Shigella dysenteriae, Pseudomonas aeruginosa) considerably declined (90-99 %) in the biofilm after seventh and fifth weeks of incubation under aerobic and anaerobic conditions, respectively, indicating a positive shift from pathogenic to beneficial microbial community. While most probable number index of fecal coliforms and E. coli in the sludge showed more reduction (98 and 99 %) under aerobic as compare to anaerobic conditions (86 and 91 %) after 9 weeks of biofilm formation on PS cubes. Correspondingly, the decreasing levels of chemical oxygen demand and biochemical oxygen demand (up to 73 %) showed signs of sludge digestion. Scanning electron microscope coupled with energy dispersive X-ray spectroscope revealed nature of PS media containing high carbon content. However, biofilm development proved to be involved in the biochemical transformation of the PS medium as indicated by Fourier transform infrared spectroscopy.

  8. Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress.

    Science.gov (United States)

    Bloomer, Richard J; Goldfarb, Allan H; Wideman, Laurie; McKenzie, Michael J; Consitt, Leslie A

    2005-05-01

    The purpose of this study was to compare oxidative modification of blood proteins, lipids, DNA, and glutathione in the 24 hours following aerobic and anaerobic exercise using similar muscle groups. Ten cross-trained men (24.3 +/- 3.8 years, [mean +/- SEM]) performed in random order 30 minutes of continuous cycling at 70% of Vo(2)max and intermittent dumbbell squatting at 70% of 1 repetition maximum (1RM), separated by 1-2 weeks, in a crossover design. Blood samples taken before, and immediately, 1, 6, and 24 hours postexercise were analyzed for plasma protein carbonyls (PC), plasma malondialdehyde (MDA), and whole-blood total (TGSH), oxidized (GSSG), and reduced (GSH) glutathione. Blood samples taken before and 24 hours postexercise were analyzed for serum 8-hydroxy-2'-deoxyguanosine (8-OHdG). PC values were greater at 6 and 24 hours postexercise compared with pre-exercise for squatting, with greater PC values at 24 hours postexercise for squatting compared with cycling (0.634 +/- 0.053 vs. 0.359 +/- 0.018 nM.mg protein(-1)). There was no significant interaction or main effects for MDA or 8-OHdG. GSSG experienced a short-lived increase and GSH a transient decrease immediately following both exercise modes. These data suggest that 30 minutes of aerobic and anaerobic exercise performed by young, cross-trained men (a) can increase certain biomarkers of oxidative stress in blood, (b) differentially affect oxidative stress biomarkers, and (c) result in a different magnitude of oxidation based on the macromolecule studied. Practical applications: While protein and glutathione oxidation was increased following acute exercise as performed in this study, future research may investigate methods of reducing macromolecule oxidation, possibly through the use of antioxidant therapy.

  9. Regulation and function of versatile aerobic and anaerobic respiratory metabolism in Pseudomonas aeruginosa

    Directory of Open Access Journals (Sweden)

    Hiroyuki eArai

    2011-05-01

    Full Text Available Pseudomonas aeruginosa is a ubiquitously distributed opportunistic pathogen that inhabits soil and water as well as animal-, human-, and plant-host-associated environments. The ubiquity would be attributed to its very versatile energy metabolism. P. aeruginosa has a highly branched respiratory chain terminated by multiple terminal oxidases and denitrification enzymes. Five terminal oxidases for aerobic respiration have been identified in the P. aeruginosa cells. Three of them, the cbb3-1 oxidase, the cbb3-2 oxidase, and the aa3 oxidase, are cytochrome c oxidases and the other two, the bo3 oxidase and the cyanide-insensitive oxidase, are quinol oxidases. Each oxidase has a specific affinity for oxygen, efficiency of energy coupling, and tolerance to various stresses such as cyanide and reactive nitrogen species. These terminal oxidases are used differentially according to the environmental conditions. P. aeruginosa also has a complete set of the denitrification enzymes that reduce nitrate to molecular nitrogen via nitrite, nitric oxide (NO, and nitrous oxide. These nitrogen oxides function as alternative electron acceptors and enable P. aeruginosa to grow under anaerobic conditions. One of the denitrification enzymes, NO reductase, is also expected to function for detoxification of NO produced by the host immune defense system. The control of the expression of these aerobic and anaerobic respiratory enzymes would contribute to the adaptation of P. aeruginosa to a wide range of environmental conditions including in the infected hosts. Characteristics of these respiratory enzymes and the regulatory system that controls the expression of the respiratory genes in the P. aeruginosa cells are overviewed in this article.

  10. Degradation of Reactive Black 5 dye using anaerobic/aerobic membrane bioreactor (MBR) and photochemical membrane reactor

    Energy Technology Data Exchange (ETDEWEB)

    You, Sheng-Jie, E-mail: sjyou@cycu.edu.tw [Department of Bioenvironmental Engineering and R and D Center for Membrane Technology, Chung Yuan Christian University, 200 Chung Pei Road, Chungli 32023, Taiwan (China); Damodar, Rahul A. [Department of Bioenvironmental Engineering and R and D Center for Membrane Technology, Chung Yuan Christian University, 200 Chung Pei Road, Chungli 32023, Taiwan (China); Hou, Sheng-Chon [Department of Civil Engineering, Chung Yuan Christian University, 200 Chung Pei Road, Chungli 32023, Taiwan (China)

    2010-05-15

    Three different types of advance treatment methods were evaluated for the degradation of Reactive Black 5 (RB5). The performance of two stage anaerobic SBR-aerobic MBR, anaerobic MBR with immobilized and suspended biocells and an integrated membrane photocatalytic reactor (MPR) using slurry UV/TiO{sub 2} system were investigated. The results suggest that, nearly 99.9% color removal and 80-95% organic COD and TOC removal can be achieved using different reactor systems. Considering the Taiwan EPA effluent standard discharge criteria for COD/TOC, the degree of treatment achieved by combining the anaerobic-aerobic system was found to be acceptable. Anew, Bacilluscereus, high color removal bacterium was isolated from Anaerobic SBR. Furthermore, when this immobilized into PVA-calcium alginate pellets, and suspended in the anaerobic MBR was able to achieve high removal efficiencies, similar to the suspended biocells system. However, the immobilized cell Anaerobic MBR was found to be more advantageous, due to lower fouling rates in the membrane unit. Results from slurry type MPR system showed that this system was capable of mineralizing RB5 dyes with faster degradation rate as compared to other systems. The reactor was also able to separate the catalyst effectively and perform efficiently without much loss of catalyst activity.

  11. Quantitative proteome and transcriptome analysis of the archaeon Thermoplasma acidophilum cultured under aerobic and anaerobic conditions.

    Science.gov (United States)

    Sun, Na; Pan, Cuiping; Nickell, Stephan; Mann, Matthias; Baumeister, Wolfgang; Nagy, István

    2010-09-03

    A comparative proteome and transcriptome analysis of Thermoplasma acidophilum cultured under aerobic and anaerobic conditions has been performed. One-thousand twenty-five proteins were identified covering 88% of the cytosolic proteome. Using a label-free quantitation method, we found that approximately one-quarter of the identified proteome (263 proteins) were significantly induced (>2 fold) under anaerobic conditions. Thirty-nine macromolecular complexes were identified, of which 28 were quantified and 15 were regulated under anaerobiosis. In parallel, a whole genome cDNA microarray analysis was performed showing that the expression levels of 445 genes were influenced by the absence of oxygen. Interestingly, more than 40% of the membrane protein-encoding genes (145 out of 335 ORFs) were up- or down-regulated at the mRNA level. Many of these proteins are functionally associated with extracellular protein or peptide degradation or ion and amino acid transport. Comparison of the transcriptome and proteome showed only a weak positive correlation between mRNA and protein expression changes, which is indicative of extensive post-transcriptional regulatory mechanisms in T. acidophilum. Integration of transcriptomics and proteomics data generated hypotheses for physiological adaptations of the cells to anaerobiosis, and the quantitative proteomics data together with quantitative analysis of protein complexes provide a platform for correlation of MS-based proteomics studies with cryo-electron tomography-based visual proteomics approaches.

  12. Partition of aerobic and anaerobic swimming costs related to gait transitions in a labriform fish

    DEFF Research Database (Denmark)

    Svendsen, Jon Christian; Tudorache, Christian; Jordan, Anders Drud;

    -c. The objective of this study was to quantify aerobic and anaerobic swimming costs at speeds below and above the Up-c in E. lateralis using swimming respirometry and video recordings to test the hypothesis that the gait transition leads to anaerobic metabolism. In this species, 1.4 body lengths per second (bl s-1......) is below the Up-c, whereas both 1.9 and 2.3 bl s-1 are above the Up-c. Exercise oxygen consumption (MO2) while the fish were swimming at these speeds was determined. The presence and magnitude of excessive post exercise oxygen consumption (EPOC) was evaluated after the three swimming speeds......% to the exercise MO2. E. lateralis switched to an unsteady burst and flap gait at 2.3 bl s-1. Burst activity correlated linearly and positively with the magnitude of the resulting EPOC. Collectively, these data suggest that steady axial propulsion does not lead to EPOC whereas transition to burst assisted swimming...

  13. Microbial degradation of chloro- and methylphenol mixtures under aerobic and denitrifying conditions. Regulation and interaction in a mixed culture; Mikrobieller Abbau von Chlor- und Methylphenolen im Gemisch unter aeroben und denitrifizierenden Bedingungen. Regulation und Interaktion in einer Mischkultur

    Energy Technology Data Exchange (ETDEWEB)

    Hollender, J.

    1994-12-31

    The mechanism and the regulation of the microbial aerobic degradation of chloro- and methylphenol mixtures were investigated by comparing the mixed culture enriched of these phenolic compounds with the isolated pure cultures. The degradation assays and the characterization of the central degrading enzymes involved show that the degradation potential of the mixed culture was essentially determined by the additive abilities of the three pure cultures Alcaligenes xylosoxidans subspecies denitrificans JH1, Pseudomonas stutzeri JH3 and Comamonas testosteroni JH5. Degradation of some substrate mixtures and avoidance of enrichment of metabolites can only be explained by the interaction of the pure cultures or the participation of other organisms in the mixed culture which were not further investigated. (orig.) [Deutsch] Bei den Untersuchungen zum Mechanismus und der Regulation des aeroben Abbaus von Chlor- und Methylphenolen im Gemisch stand der Vergleich zwischen der auf diesen Phenolen angereicherten Mischkultur und den daraus isolierten Reinkulturen im Vordergrund. Die Abbauversuche und die Charakterisierung der am Metabolismus beteiligten zentralen Enzyme zeigen, dass fuer das Abbaupotential der Mischkultur im wesentlichen die additiven Abbauleistungen der drei Reinkulturen Alcaligenes xylosoxidans subspecies dentrificans JH1, Pseudomonas stutzeri JH3 und Comamonas testosteroni JH5 verantwortlich sind. Der Abbau einiger Substratmischungen und die Vermeidung einer Anreicherung von Metaboliten in der Mischkultur sind jedoch nur durch eine Interaktion der Reinkulturen oder durch eine Beteiligung anderer nicht naeher untersuchter Organismen in der Mischkultur zu erklaeren. (orig.)

  14. Understanding the removal mechanisms of PPCPs and the influence of main technological parameters in anaerobic UASB and aerobic CAS reactors

    Energy Technology Data Exchange (ETDEWEB)

    Alvarino, T., E-mail: teresa.alvarino@usc.es; Suarez, S., E-mail: Sonia.suarez@usc.es; Lema, J.M., E-mail: juan.lema@usc.es; Omil, F., E-mail: francisco.omil@usc.es

    2014-08-15

    Highlights: • Removals of 16 PPCPs under aerobic and anaerobic conditions were quantified. • Operation conditions (HRT, v{sub up}, biomass activity and conformation) influenced removal. • Highest removals associated to aerobic biodegradation. • Sorption was only relevant for lipophilic compounds in the UASB reactor. - Abstract: The removal of 16 Pharmaceutical and Personal Care Products (PPCPs) were studied in a conventional activated sludge (CAS) unit and an upflow anaerobic sludge blanket (UASB) reactor. Special attention was paid to each biomass conformation and activity as well as to operational conditions. Biodegradation was the main PPCP removal mechanism, being higher removals achieved under aerobic conditions, except in the case of sulfamethoxazole and trimetrophim. Under anaerobic conditions, PPCP biodegradation was correlated with the methanogenic rate, while in the aerobic reactor a relationship with nitrification was found. Sorption onto sludge was influenced by biomass conformation, being only significant for musk fragrances in the UASB reactor, in which an increase of the upward velocity and hydraulic retention time improved this removal. Additionally, PPCP sorption increased with time in the UASB reactor, due to the granular biomass structure which suggests the existence of intra-molecular diffusion.

  15. Enhanced Biological Phosphorus Removal in Anaerobic/Aerobic Sequencing Batch Reactor Supplied with Glucose as Carbon Source

    Institute of Scientific and Technical Information of China (English)

    LIU Yanan; YU Shui-li; JING Guo-lin; ZHAO Bing-jie; GUO Si-yuan

    2005-01-01

    Phosphorus removal performance in an aerobic/aerobic sequencing batch reactor (SBR) supplied with glucose as carbon source was investigated. It was found that there was no phosphate release concomitant with the storing of poly-β-hydroxyalkanoate (PHA) during the anaerobic phase. Whereas, glycogen was soon built up followed by rapid consumption, at the same time, glucose was depleted rapidly. Based on the analysis of different fractions of phosphorus in activated sludge, the relative ratio of organically bound phosphorus in sludge changed at the end of anaerobic and aerobic phases. The ratios were 45.3% and51.8% respectively. This showed that the polyphosphate broke down during the anaerobic phase to supply part of energy for PHA synthesis. The reason why there was no phosphate release might be the biosorption effect of extracellular exopolymers (EPS). It was also proved by the analysis of EPS with scanning electron microscopy (SEM)combined with energy dispersive spectrometry (EDS). The phosphorus weight percentage of EPS at the end of anaerobic phase was 9.22%.

  16. An integrated anaerobic/aerobic bioprocess for the remediation of chlorinated phenol-contaminated soil and groundwater.

    Science.gov (United States)

    Ehlers, George A; Rose, Peter D

    2006-07-01

    An investigation of biodegradation of chlorinated phenol in an anaerobic/aerobic bioprocess environment was made. The reactor configuration used consisted of linked anaerobic and aerobic reactors, which served as a model for a proposed bioremediation strategy. The proposed strategy was studied in two reactors before linkage. In the anaerobic compartment, the transformation of the model contaminant, 2,4,6-trichlorophenol (2,4,6-TCP), to lesser-chlorinated metabolites was shown to occur during reductive dechlorination under sulfate-reducing conditions. The consortium was also shown to desorb and mobilize 2,4,6-TCP in soils. This was followed, in the aerobic compartment, by biodegradation of the pollutant and metabolites, 2,4-dichlorophenol, 4-chlorophenol, and phenol, by immobilized white-rot fungi. The integrated process achieved elimination of the compound by more than 99% through fungal degradation of metabolites produced in the dechlorination stage. pH correction to the anaerobic reactor was found to be necessary because acidic effluent from the fungal reactor inhibited sulfate reduction and dechlorination.

  17. Efficacy of a Ventilatory Training Mask to Improve Anaerobic and Aerobic Capacity in Reserve Officers' Training Corps Cadets.

    Science.gov (United States)

    Sellers, John H; Monaghan, Taylor P; Schnaiter, Jessica A; Jacobson, Bert H; Pope, Zachary K

    2016-04-01

    The purpose of this study was to examine the efficacy of a ventilatory training mask to improve anaerobic and aerobic fitness in reserve officers' training corps (ROTC) cadets. Seventeen ROTC cadets from a Midwest university completed pre- and postassessments consisting of anthropometry, a 30-second Wingate Anaerobic Test (WAnT), and a maximal aerobic capacity test (V[Combining Dot Above]O2max). A 6-week intervention training period was used during which time participants completed their mandatory physical training (PT) sessions. Participants were randomly assigned to either the experimental group (MASK; n = 9) or the control group (CON; n = 8). The ventilatory training masks were adjusted to simulate an altitude of 2,750 m. There was no significant effect (p ≤ 0.05) between groups on fatigue index, anaerobic capacity, peak power, V[Combining Dot Above]O2max, or time to exhaustion. These results suggest that the use of the ventilatory training mask during mandatory PT did not elicit superior aerobic or anaerobic adaptations in ROTC cadets. Therefore, it is recommended that more established simulated altitude training methods be used when incorporating intermittent hypoxic training.

  18. Yolo County's Accelerated Anaerobic and Aerobic Composting (Full-Scale Controlled Landfill Bioreactor) Project

    Science.gov (United States)

    Yazdani, R.; Kieffer, J.; Akau, H.; Augenstein, D.

    2002-12-01

    Sanitary landfilling is the dominant method of solid waste disposal in the United States, accounting for about 217 million tons of waste annually (U.S. EPA, 1997) and has more than doubled since 1960. In spite of increasing rates of reuse and recycling, population and economic growth will continue to render landfilling as an important and necessary component of solid waste management. Yolo County Department of Planning and Public Works, Division of Integrated Waste Management is demonstrating a new landfill technology called Bioreactor Landfill to better manage solid waste. In a Bioreactor Landfill, controlled quantities of liquid (leachate, groundwater, gray-water, etc.) are added and recirculated to increase the moisture content of the waste and improve waste decomposition. As demonstrated in a small-scale demonstration project at the Yolo County Central Landfill in 1995, this process significantly increases the biodegradation rate of waste and thus decreases the waste stabilization and composting time (5 to 10 years) relative to what would occur within a conventional landfill (30 to 50 years or more). When waste decomposes anaerobically (in absence of oxygen), it produces landfill gas (biogas). Biogas is primarily a mixture of methane, a potent greenhouse gas, carbon dioxide, and small amounts of Volatile Organic Compounds (VOC's) which can be recovered for electricity or other uses. Other benefits of a bioreactor landfill composting operation include increased landfill waste settlement which increases in landfill capacity and life, improved leachate chemistry, possible reduction of landfill post-closure management time, opportunity to explore decomposed waste for landfill mining, and abatement of greenhouse gases through highly efficient methane capture over a much shorter period of time than is typical of waste management through conventional landfilling. This project also investigates the aerobic decomposition of waste of 13,000 tons of waste (2.5 acre) for

  19. A self-sustaining advanced lignocellulosic biofuel production by integration of anaerobic digestion and aerobic fungal fermentation.

    Science.gov (United States)

    Zhong, Yuan; Ruan, Zhenhua; Zhong, Yingkui; Archer, Steven; Liu, Yan; Liao, Wei

    2015-03-01

    High energy demand hinders the development and application of aerobic microbial biofuel production from lignocellulosic materials. In order to address this issue, this study focused on developing an integrated system including anaerobic digestion and aerobic fungal fermentation to convert corn stover, animal manure and food wastes into microbial lipids for biodiesel production. Dairy manure and food waste were first anaerobically digested to produce energy and solid digestate (AD fiber). AD fiber and corn stover were then processed by a combined alkali and acid hydrolysis, followed by fungal lipid accumulation. The integrated process can generate 1L biodiesel and 1.9 kg methane from 12.8 kg dry dairy manure, 3.1 kg dry food wastes and 12.2 kg dry corn stover with a positive net energy of 57 MJ, which concludes a self-sustaining lignocellulosic biodiesel process and provides a new route to co-utilize corn stover and organic wastes for advanced biofuel production.

  20. In vitro aerobic and anaerobic muscle capacities in the European eel, Anguilla anguilla: effects of a swimming session.

    Science.gov (United States)

    Sébert, Philippe; Mortelette, Hélène; Nicolas, Jonathan; Amérand, Aline; Belhomme, Marc; Moisan, Christine

    2011-05-31

    In order to have a general view of metabolic requirements during swimming, in vitro aerobic and anaerobic fluxes were measured in red and white muscles from silver eels and yellow eels which differ in activity levels and nutritional states. These measurements were performed in control eels and after a 4 day swimming session (70% U(crit) in yellow eels, 80% U(crit) in silver eels). A swimming session significantly increases U(crit) from 12% to 18%, depending on the stage, with a significantly higher in vitro energy cost during the yellow stage at the muscle level. In vitro, the swimming session brings about a gain in anaerobic capacities rather than in aerobic ones. Some in vivo hypotheses are proposed.

  1. Performance evaluation of an anaerobic/aerobic landfill-based digester using yard waste for energy and compost production.

    Science.gov (United States)

    Yazdani, Ramin; Barlaz, Morton A; Augenstein, Don; Kayhanian, Masoud; Tchobanoglous, George

    2012-05-01

    The objective of this study was to evaluate a new alternative for yard waste management by constructing, operating and monitoring a landfill-based two-stage batch digester (anaerobic/aerobic) with the recovery of energy and compost. The system was initially operated under anaerobic conditions for 366 days, after which the yard waste was aerated for an additional 191 days. Off gas generated from the aerobic stage was treated by biofilters. Net energy recovery was 84.3MWh, or 46kWh per million metric tons of wet waste (as received), and the biochemical methane potential of the treated waste decreased by 83% during the two-stage operation. The average removal efficiencies of volatile organic compounds and non-methane organic compounds in the biofilters were 96-99% and 68-99%, respectively.

  2. Aerobic and anaerobic changes with high-intensity interval training in active college-aged men.

    Science.gov (United States)

    Ziemann, Ewa; Grzywacz, Tomasz; Łuszczyk, Marcin; Laskowski, Radoslaw; Olek, Robert A; Gibson, Ann L

    2011-04-01

    We investigated the aerobic and anaerobic benefits of high-intensity interval training performed at a work-to-rest ratio of 1:2 because little performance enhancement data exist based on this ratio. Recreationally active male volunteers (21 years, 184 cm, 81.5 kg) were randomly assigned to a training (interval training [IT] n = 10) or control group (n = 11). Baseline assessments were repeated after the last training session. Each participant underwent basic anthropometric assessment and performed a VO2max test on an electronically braked cycle ergometer and a 30-second Wingate test. Venous samples were acquired at the antecubital vein and subsequently processed for lactate (LA); samples were obtained at rest, and 5 and 15-minute post-Wingate test. The interval training used a cycling power output equivalent to 80% of VO2max (80% p VO2max) applied for 6 90-second bouts (each followed by 180-second rest) per session, 3 sessions per week, for 6 weeks. The control group maintained their normal routine for the 6-week period. Group × time repeated-measures analyses of variance revealed that IT improved VO2max (5.5 ml · kg(-1) · min), anaerobic threshold (3.8 ml · kg(-1) · min), work output (12.5 J · kg(-1)), glycolytic work (11.5 J · kg(-1)), mean power (0.3 W · kg), peak power (0.4 W · kg(-1)), and max power (0.4 W · kg(-1)); p training program may rapidly restore or improve a client's or athlete's maximal functional capacity.

  3. Anaerobic/aerobic treatment of greywater via UASB and MBR for unrestricted reuse.

    Science.gov (United States)

    Abdel-Shafy, Hussein I; Al-Sulaiman, Ahmed Makki; Mansour, Mona S M

    2015-01-01

    The aim of the present study was to investigate the efficiency of integrated up-flow anaerobic sludge blanket (UASB) as anaerobic system followed by membrane bioreactor (MBR) as aerobic system for the treatment of greywater for unrestricted reuse. Pilot-scale UASB and MBR units were installed and operated in the NRC, Egypt. Real raw greywater was subjected to UASB and the effluent was further treated with microfiltration MBR. The necessary trans-membrane pressure difference is applied by the water head above the membrane (gravity flow) without any energy input. The average characteristics of the raw greywater were 95, 392, 298, 10.45, 0.4, 118.5 and 28 mg/L for total suspended solids (TSS), chemical oxygen demand (COD), biochemical oxygen demand (BOD), total phosphates, nitrates, oil and grease, and total Kjeldahl nitrogen (TKN), respectively. The pH was 6.71. The UASB treatment efficiency reached 19.3, 57.8, 67.5 and 83.7% for TSS, COD, BOD5 and oil and grease, respectively. When the UASB effluent was further treated with MBR, the overall removal rate achieved 97.7, 97.8, 97.4 and 95.8% for the same parameters successively. The characteristics of the final effluent reached 2.5, 8.5, 6.1, 0.95, 4.6 and 2.3 mg/L for TSS, COD, BOD, phosphates, oil and grease and TKN, respectively. This final treated effluent could cope with the unrestricted water reuse of local Egyptian guidelines.

  4. Comprehensive microbial analysis of combined mesophilic anaerobic-thermophilic aerobic process treating high-strength food wastewater.

    Science.gov (United States)

    Jang, Hyun Min; Ha, Jeong Hyub; Park, Jong Moon; Kim, Mi-Sun; Sommer, Sven G

    2015-04-15

    A combined mesophilic anaerobic-thermophilic aerobic process was used to treat high-strength food wastewater in this study. During the experimental period, most of solid residue from the mesophilic anaerobic reactor (R1) was separated by centrifugation and introduced into the thermophilic aerobic reactor (R2) for further digestion. Then, thermophilic aerobically-digested sludge was reintroduced into R1 to enhance reactor performance. The combined process was operated with two different Runs: Run I with hydraulic retention time (HRT) = 40 d (corresponding OLR = 3.5 kg COD/m(3) d) and Run II with HRT = 20 d (corresponding OLR = 7 kg COD/m(3)). For a comparison, a single-stage mesophilic anaerobic reactor (R3) was operated concurrently with same OLRs and HRTs as the combined process. During the overall digestion, all reactors showed high stability without pH control. The combined process demonstrated significantly higher organic matter removal efficiencies (over 90%) of TS, VS and COD and methane production than did R3. Quantitative real-time PCR (qPCR) results indicated that higher populations of both bacteria and archaea were maintained in R1 than in R3. Pyrosequencing analysis revealed relatively high abundance of phylum Actinobacteria in both R1 and R2, and a predominance of phyla Synergistetes and Firmicutes in R3 during Run II. Furthermore, R1 and R2 shared genera (Prevotella, Aminobacterium, Geobacillus and Unclassified Actinobacteria), which suggests synergy between mesophilic anaerobic digestion and thermophilic aerobic digestion. For archaea, in R1 methanogenic archaea shifted from genus Methanosaeta to Methanosarcina, whereas genera Methanosaeta, Methanobacterium and Methanoculleus were predominant in R3. The results demonstrated dynamics of key microbial populations that were highly consistent with an enhanced reactor performance of the combined process.

  5. Aerobic and anaerobic enzymatic activity of orange roughy (Hoplostethus atlanticus) and alfonsino (Beryx splendens) from the Juan Fernandez seamounts area.

    Science.gov (United States)

    Saavedra, L M; Quiñones, R A; Gonzalez-Saldía, R R; Niklitschek, E J

    2016-06-01

    The aerobic and anaerobic enzymatic activity of two important commercial bathypelagic species living in the Juan Fernández seamounts was analyzed: alfonsino (Beryx splendens) and orange roughy (Hoplostethus atlanticus). These seamounts are influenced by the presence of an oxygen minimum zone (OMZ) located between 160 and 250 m depth. Both species have vertical segregation; alfonsino is able to stay in the OMZ, while orange roughy remains at greater depths. In this study, we compare the aerobic and anaerobic capacity of these species, measuring the activity of key metabolic enzymes in different body tissues (muscle, heart, brain and liver). Alfonsino has higher anaerobic potential in its white muscle due to greater lactate dehydrogenase (LDH) activity (190.2 μmol NADH min(-1) g ww(-1)), which is related to its smaller body size, but it is also a feature shared with species that migrate through OMZs. This potential and the higher muscle citrate synthase and electron transport system activities indicate that alfonsino has greater swimming activity level than orange roughy. This species has also a high MDH/LDH ratio in its heart, brain and liver, revealing a potential capacity to conduct aerobic metabolism in these organs under prolonged periods of environmental low oxygen conditions, preventing lactic acid accumulation. With these metabolic characteristics, alfonsino may have increased swimming activity to migrate and also could stay for a period of time in the OMZ. The observed differences between alfonsino and orange roughy with respect to their aerobic and anaerobic enzymatic activity are consistent with their characteristic vertical distributions and feeding behaviors.

  6. Effects of aerobic interval training versus continuous moderate exercise programme on aerobic and anaerobic capacity, somatic features and blood lipid profile in collegate females

    Directory of Open Access Journals (Sweden)

    Krzystof Mazurek

    2014-11-01

    Full Text Available introduction. Regular physical activity has many positive health benefits, including reducing the risk of cardiovascular diseases, metabolic diseases and some cancers, as well as improving the quality of life. objectives. The aim of the study was to examine the effects of 8-week aerobic interval cycle exercise training (AIT compared to continuous cycle exercises of moderate intensity (CME on the aerobic and anaerobic capacity, somatic features and lipid profile. material and methods. The research was conducted in 88 volunteers aged 19.5±0.6 years, who were randomized to three groups of organized physical activity (OPA, who exercised 3 times per week in 47 min sessions: (I AIT (n=24 comprising 2 series of 6x10 s sprinting with maximal pedalling cadence and active rest pedalling with intensity 65%–75% HRmax, (II CME (n=22 corresponding to 65%-75% HRmax, (III regular collegiate physical education classes of programmed exercises (CON; n=42. Before and after OPA anthropometrics, aero- and anaerobic capacity and lipid profile indices were measured. results. In AIT, a significantly greater decrease of waist circumference and WHR was noted when compared to CON, and a significantly greater reduction of sum of skinfolds than in CON and CME. Improvement in relative and absolute VO2max (L/min and ml/kg/min was significantly higher in AIT than CON. Work output and peak power output in the anaerobic test improved significantly in AIT, CME and CON, but independently of training type. OPA was effective only in reducing triglyceride concentrations in CME and CON groups, without interaction effects in relation to training type. conclusion. It was found that 8 weeks of OPA was beneficial in improving somatic and aerobic capacity indices, but AIT resulted in the greatest improvement in somatic indices (waist circumference, WHR, sum of skinfolds and in VO[sub]2[/sub]max, compared to CME and CON programmes.

  7. The relationship between repeated sprint ability and the aerobic and anaerobic energy systems.

    Science.gov (United States)

    Wadley, G; Le Rossignol, P

    1998-06-01

    A large number of team games require participants to repeatedly produce maximal or near maximal sprints of short duration with brief recovery periods. The purpose of the present study was to determine the relationship between a repeated sprint ability (RSA) test that is specific to the energy demands of Australian Rules football (ARF), and the aerobic and anaerobic energy systems. Seventeen ARF players participated in the study. Each participant was assessed for VO2 max, accumulated oxygen deficit (AOD), best 20 m sprint time and RSA. The RSA test involved 12x20 m sprints departing every 20 s. When including the work performed during the time taken to decelerate, the test involved a work to rest ratio of approximately 1:3. Total sprinting time and the percentage decrement of repeated sprinting times were the two derived measures of RSA. The results indicate that the best 20 m sprint time was the only factor to correlate significantly with total sprinting time (r = 0.829, P RSA test. This was interpreted to signify that the phosphagen system was the major energy contributor for this test.

  8. Comprehensive analysis of aerobic and anaerobic bacteria found on dental bib clips.

    Science.gov (United States)

    Alt-Holland, Addy; Murphy, Christina M; Powers, Anne; Kublin, Claire L; Jeong, Youjin Natalie; DiMattia, Michelle; Pham, Linh; Park, Angel; Finkelman, Matthew; Lombard, Maureen; Hanley, James B; Paster, Bruce J; Kugel, Gerard

    2013-04-01

    Multiple-use dental bib clips are considered to present relatively low risks for transmitting infections and, thus, are thought to only require disinfection between patient visits. This study was designed to: 1) determine the presence and composition of bacterial contaminants on reusable rubber-faced metal bib clips after dental treatment at the hygiene clinic at Tufts University School of Dental Medicine and 2) evaluate the effectiveness of the disinfection for this clip type. Aerobic and anaerobic bacterial contaminant loads on the surfaces of the clips were investigated immediately after hygiene treatments were rendered and again after clips were disinfected. The species and strains of bacterial isolates were identified using 16S rDNA sequencing and Human Oral Microbe Identification Microarray analyses. The results demonstrated that although the use of disinfection proved to be significantly effective, some clips retained at least one bacterium on their surfaces after disinfection. Although the bacterial species present on disinfected clips were typical skin or environmental isolates, some were oral in origin. In the study's settings, bacterial presence on the clips did not indicate an infectious disease problem. The different bacterial loads on clips suggest that cross-contamination risks may not be the same for all clinics, and that this difference may be related to the type of treatments and services performed.

  9. Performances of anaerobic and aerobic membrane bioreactors for the treatment of synthetic textile wastewater.

    Science.gov (United States)

    Yurtsever, Adem; Sahinkaya, Erkan; Aktaş, Özgür; Uçar, Deniz; Çınar, Özer; Wang, Zhiwei

    2015-09-01

    This study aims at comparatively evaluating anaerobic and aerobic MBRs for the treatment of azo-dye containing synthetic wastewater. Also, the filtration performances of AnMBR and AeMBR were compared under similar operating conditions. In both MBRs, high COD removal efficiencies were observed. Although almost complete color removal was observed in AnMBR, only partial (30-50%) color removal was achieved in AeMBR. AnMBR was successfully operated up to 9 L/(m(2)h) (LMH) and no chemical cleaning was required at 4.5 LMH for around 50 days. AeMBR was operated successfully up to 20 LMH. The filtration resistance of AnMBR was generally higher compared to AeMBR although reversible fouling rates were comparable. In both MBRs, offline chemical cleaning with NaOCl and sulfuric acid almost completely removed irreversible fouling and the resistances of chemically cleaned membranes were close to those of new membranes.

  10. The Effects of High Intensity Interval Training vs Steady State Training on Aerobic and Anaerobic Capacity

    Directory of Open Access Journals (Sweden)

    Carl Foster, Courtney V. Farland, Flavia Guidotti, Michelle Harbin, Brianna Roberts, Jeff Schuette, Andrew Tuuri, Scott T. Doberstein, John P. Porcari

    2015-12-01

    Full Text Available High intensity interval training (HIIT has become an increasingly popular form of exercise due to its potentially large effects on exercise capacity and small time requirement. This study compared the effects of two HIIT protocols vs steady-state training on aerobic and anaerobic capacity following 8-weeks of training. Fifty-five untrained college-aged subjects were randomly assigned to three training groups (3x weekly. Steady-state (n = 19 exercised (cycle ergometer 20 minutes at 90% of ventilatory threshold (VT. Tabata (n = 21 completed eight intervals of 20s at 170% VO2max/10s rest. Meyer (n = 15 completed 13 sets of 30s (20 min @ 100% PVO2 max/ 60s recovery, average PO = 90% VT. Each subject did 24 training sessions during 8 weeks. Results: There were significant (p < 0.05 increases in VO2max (+19, +18 and +18% and PPO (+17, +24 and +14% for each training group, as well as significant increases in peak (+8, + 9 and +5% & mean (+4, +7 and +6% power during Wingate testing, but no significant differences between groups. Measures of the enjoyment of the training program indicated that the Tabata protocol was significantly less enjoyable (p < 0.05 than the steady state and Meyer protocols, and that the enjoyment of all protocols declined (p < 0.05 across the duration of the study. The results suggest that although HIIT protocols are time efficient, they are not superior to conventional exercise training in sedentary young adults.

  11. The Effects of High Intensity Interval Training vs Steady State Training on Aerobic and Anaerobic Capacity

    Science.gov (United States)

    Foster, Carl; Farland, Courtney V.; Guidotti, Flavia; Harbin, Michelle; Roberts, Brianna; Schuette, Jeff; Tuuri, Andrew; Doberstein, Scott T.; Porcari, John P.

    2015-01-01

    High intensity interval training (HIIT) has become an increasingly popular form of exercise due to its potentially large effects on exercise capacity and small time requirement. This study compared the effects of two HIIT protocols vs steady-state training on aerobic and anaerobic capacity following 8-weeks of training. Fifty-five untrained college-aged subjects were randomly assigned to three training groups (3x weekly). Steady-state (n = 19) exercised (cycle ergometer) 20 minutes at 90% of ventilatory threshold (VT). Tabata (n = 21) completed eight intervals of 20s at 170% VO2max/10s rest. Meyer (n = 15) completed 13 sets of 30s (20 min) @ 100% PVO2 max/ 60s recovery, average PO = 90% VT. Each subject did 24 training sessions during 8 weeks. Results: There were significant (p HIIT protocols are time efficient, they are not superior to conventional exercise training in sedentary young adults. Key points Steady state training equivalent to HIIT in untrained students Mild interval training presents very similar physiologic challenge compared to steady state training HIIT (particularly very high intensity variants were less enjoyable than steady state or mild interval training Enjoyment of training decreases across the course of an 8 week experimental training program PMID:26664271

  12. Mineralization of 14C-ring Labelled 2,4-D in Egyptian Soils Under Aerobic and Anaerobic Conditions

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Obiectives To study the mineralization of 2,4-D in clay and clay loam Egyptian soils under subtropical conditions over a period of 90 d. Methods Using 14C-ring labelled pesticide, laboratory studies under aerobic and anaerobic conditions were conducted. 14C-activity in solutions was directly determined by liquid scintillation counting. Unextractable soil residues were determined by combustion.The nature of methanolic 14C-residues was determined by thin layer and high performance liquid chromatographic analysis. Results Under aerobic conditions 10%-14% of applied dose was mineralized during 90 d irrespective of soil type. The soil extractable pesticide residues decreased with time and the bound residues gradually increased. The highest binding capacity of about 26%-29% was observed in clay soil under aerobic conditions after 90 d. A good balance sheet was obtained and the percentage recovery was generally between 91% and 100%. Conclusion The mineralization of 2,4-D in clay soil was higher than that in clay loam soil under anaerobic conditions.Under aerobic conditions, the soil type had no influence on mineralizaion capacity of 2,4-D during 90 d. The soil binding increased with time whereby the extractable 14C-residues simultaneously decreased.Chromatographic analysis of the methanol extractable 14C-residues of soils revealed the presence of 2,4-D as a main product together with 2,4-dichlorophenol.

  13. The effects of aerobic and anaerobic training on fitness, blood pressure, and psychological stress and well-being.

    Science.gov (United States)

    Norris, R; Carroll, D; Cochrane, R

    1990-01-01

    To determine whether fitness alters psychological and physiological indices of well-being, male police officers were assigned to either an aerobic or anaerobic training condition or to a no treatment control group. The training groups met three times per week in 45 min sessions aimed at improving either cardiovascular endurance or muscle strength. Aerobic fitness level, heart rate, blood pressure and self-report of stress and well-being were measured prior to and following 10 weeks of training. Post-training fitness measures confirmed the effectiveness of training and between group differences for physiological and self-report measures were found. Subjects undergoing aerobic training evinced larger changes on the self-report measures of well-being and stress than the anaerobic trainers and both groups showed significant improvement when compared to controls. This experiment provides support for the hypothesis that exercise, and in particular aerobic exercise, has positive effects of well-being. It is suggested that future research might usefully explore the particular contribution of different aspects of the training situation to these effects.

  14. Sequential anaerobic-aerobic decolourization of a real textile wastewater in a two-phase partitioning bioreactor.

    Science.gov (United States)

    Tomei, M Concetta; Mosca Angelucci, Domenica; Daugulis, Andrew J

    2016-12-15

    This work describes the application of a solid-liquid two-phase partitioning bioreactor (TPPB) for the removal of colour from a real textile wastewater containing reactive azo-dyes. Four polymers were tested over the pH range of 4-9 to select the most effective absorbant to be used as the partitioning phase in the TPPB. The best results were obtained with Hytrel 8206 at pH4 achieving ~70% colour removal, based on the dominant wavelength, in the first 5h of contact time, and 84% after 24h. Wastewater treatment was undertaken in a solid-liquid TPPB operated with Hytrel 8206 in sequential anaerobic-aerobic configuration. The reaction time of 23h was equally distributed between the anaerobic and aerobic phases and, to favour colour uptake, the pH was controlled at 4.5 in the first 4h of the anaerobic phase, and then increased to 7.5. Colour removal (for the dominant wavelength, 536nm) increased from 70 to 85% by modifying the bioreactor operation from single-phase to TPPB mode. Based on COD measurements nearly complete biodegradation of the intermediates produced in the anaerobic phase was obtained, both in the single-phase and two-phase mode, with better performance of the TPPB system reaching 75% CODDye removal.

  15. Aerobic and anaerobic metabolism in oxygen minimum layer fishes: the role of alcohol dehydrogenase.

    Science.gov (United States)

    Torres, Joseph J; Grigsby, Michelle D; Clarke, M Elizabeth

    2012-06-01

    Zones of minimum oxygen form at intermediate depth in all the world's oceans as a result of global circulation patterns that keep the water at oceanic mid-depths out of contact with the atmosphere for hundreds of years. In areas where primary production is very high, the microbial oxidation of sinking organic matter results in very low oxygen concentrations at mid-depths. Such is the case with the Arabian Sea, with O(2) concentrations reaching zero at 200 m and remaining very low (fishes (primarily lanternfishes: Mytophidae) inhabiting the Arabian Sea and California borderland perform a daily vertical migration into the low-oxygen layer, spending daylight hours in the oxygen minimum zone and migrating upward into normoxic waters at night. To find out how fishes were able to survive their daily sojourns into the minimum zone, we tested the activity of four enzymes, one (lactate dehydrogenase, LDH) that served as a proxy for anaerobic glycolysis with a conventional lactate endpoint, a second (citrate synthase, CS) that is indicative of aerobic metabolism, a third (malate dehydrogenase) that functions in the Krebs' cycle and as a bridge linking mitochondrion and cytosol, and a fourth (alcohol dehydrogenase, ADH) that catalyzes the final reaction in a pathway where pyruvate is reduced to ethanol. Ethanol is a metabolic product easily excreted by fish, preventing lactate accumulation. The ADH pathway is rarely very active in vertebrate muscle; activity has previously been seen only in goldfish and other cyprinids capable of prolonged anaerobiosis. Activity of the enzyme suite in Arabian Sea and California fishes was compared with that of ecological analogs in the same family and with the same lifestyle but living in systems with much higher oxygen concentrations: the Gulf of Mexico and the Southern Ocean. ADH activities in the Arabian Sea fishes were similar to those of goldfish, far higher than those of confamilials from the less severe minimum in the Gulf of Mexico

  16. Hydrodynamic behavior of a combined anaerobic-aerobic system employed in the treatment of vinasse

    Directory of Open Access Journals (Sweden)

    Fátima Resende Luiz Fia

    Full Text Available ABSTRACT The understanding of reactor hydrodynamics is essential for improving the performance of a reactor and biogas production. This study sought to evaluate the hydrodynamic behavior of a combined anaerobic-aerobic system at a laboratory scale for treating vinasse. The experiment was conducted in a system using two UASB reactors connected in series, followed by an Aerated Submerged Biological Filter (ASBF. The flow rates applied to the system and the corresponded theoretical HRTs in the UASB1, UASB2 and ASBF were respectively: 15.6 L d-1 and 1.2 d, 1.2 d and 1.0 d (Phase I - first year; 8.5 L d-1 and 2.1 d; 2.1 d and 1.8 d (Phase II - first year, 14.6 L d-1 and 1.1 d, 1.1 d and 1.0 d (Phase III - second year; 29.5 L d-1 and 0.6 d, 0.6 d and 0.5 d (Phase IV - second year. The hydrodynamic studies were carried out using pulse type stimulus-response tests, employing LiCl as a tracer. The coefficients of determination for the dispersion models (R2 indicate a close approximation of a continuous stirred tank reactor in series (multi-CSTR model, with an average of 2.5, 2.3 and 1.2 (first year, and 1.1, 1.4 and 0.9 (second year multi-CSTR for UASB1, UASB2 and ASBF, respectively. Results of the hydrodynamic tests carried out in UASB1, UASB2 and ASBF indicated strong tendency for flow in the complete mixture hydraulic regime, detecting a wide dispersion in the units, in addition to the presence of short circuits and dead zones.

  17. Repeated sprint ability in elite water polo players and swimmers and its relationship to aerobic and anaerobic performance.

    Science.gov (United States)

    Meckel, Yoav; Bishop, David; Rabinovich, Moran; Kaufman, Leonid; Nemet, Dan; Eliakim, Alon

    2013-01-01

    The purpose of this study was to determine indices of swimming repeated sprint ability (RSA) in 19 elite water polo players compared to 16 elite swimmers during a repeated sprint swimming test (RST), and to examine the relationships between these indices and aerobic and anaerobic performance capabilities in both groups. Indices of RSA were determined by the ideal sprint time (IS), the total sprint time (TS), and the performance decrement (PD) recorded during an 8 x 15-m swimming RST. Single long - (800-m) and short-(25-m) distance swim tests were used to determined indices of aerobic and anaerobic swimming capabilities, respectively. The water polo players exhibited lower RSA swimming indices, as well as lower scores in the single short and long swim distances, compared to the swimmers. Significant relationships were found between the 25- m swim results and the IS and the TS, but not the PD of both the swimmers and the water polo players. No significant relationships were found between the 800-m swim results and any of the RSA indices in either the swimmers or the water polo players. No significant relationships were found between the 25-m and the 800-m swim results in either the swimmers or the water polo players. The results indicate that swimmers posses better RSA as well as higher anaerobic and aerobic capabilities, as reflected by the single short- and long-distance swim tests, compared to water polo players. The results also indicate that, as for running and cycling, repeated sprint swim performance is strongly related to single sprint performance. Key PointsElite water polo players demonstrated lower repeated sprint ability (RSA), aerobic and anaerobic capabilities compared to elite swimmers.A 25-m swim trial correlated significantly with ideal sprint time and total sprint time, emphasizing the important contribution of anaerobic metabolism for these exercise types in both water polo players and swimmers.800-m swim results did not correlate with RSA or 25-m

  18. Biological degradation of triclocarban and triclosan in a soil under aerobic and anaerobic conditions and comparison with environmental fate modelling

    Energy Technology Data Exchange (ETDEWEB)

    Ying Guangguo [State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640 (China); CSIRO Land and Water, Adelaide Laboratory, PMB2, Glen Osmond SA 5064 (Australia)], E-mail: guang-guo.ying@gig.ac.cn; Yu Xiangyang [CSIRO Land and Water, Adelaide Laboratory, PMB2, Glen Osmond SA 5064 (Australia); Food Safety Research Institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014 (China); Kookana, Rai S. [CSIRO Land and Water, Adelaide Laboratory, PMB2, Glen Osmond SA 5064 (Australia)

    2007-12-15

    Triclocarban and triclosan are two antimicrobial agents widely used in many personal care products. Their biodegradation behaviour in soil was investigated by laboratory degradation experiments and environmental fate modelling. Quantitative structure-activity relationship (QSAR) analyses showed that triclocarban and triclosan had a tendency to partition into soil or sediment in the environment. Fate modelling suggests that either triclocarban or triclosan 'does not degrade fast' with its primary biodegradation half-life of 'weeks' and ultimate biodegradation half-life of 'months'. Laboratory experiments showed that triclocarban and triclosan were degraded in the aerobic soil with half-life of 108 days and 18 days, respectively. No negative effect of these two antimicrobial agents on soil microbial activity was observed in the aerobic soil samples during the experiments. But these two compounds persisted in the anaerobic soil within 70 days of the experimental period. - Triclocarban and triclosan can be degraded by microbial processes in aerobic soil, but will persist in anaerobic soil.

  19. The relationship of aerobic capacity, anaerobic peak power and experience to performance in CrossFit exercise.

    Science.gov (United States)

    Bellar, D; Hatchett, A; Judge, L W; Breaux, M E; Marcus, L

    2015-11-01

    CrossFit is becoming increasingly popular as a method to increase fitness and as a competitive sport in both the Unites States and Europe. However, little research on this mode of exercise has been performed to date. The purpose of the present investigation involving experienced CrossFit athletes and naïve healthy young men was to investigate the relationship of aerobic capacity and anaerobic power to performance in two representative CrossFit workouts: the first workout was 12 minutes in duration, and the second was based on the total time to complete the prescribed exercise. The participants were 32 healthy adult males, who were either naïve to CrossFit exercise or had competed in CrossFit competitions. Linear regression was undertaken to predict performance on the first workout (time) with age, group (naïve or CrossFit athlete), VO2max and anaerobic power, which were all significant predictors (p CrossFit experience as a significant predictor (p CrossFit competition is a key component of performance in CrossFit workouts which are representative of those performed in CrossFit, and that, in at least one these workouts, aerobic capacity and anaerobic power are associated with success.

  20. Repeated Sprint Ability in Elite Water Polo Players and Swimmers and its Relationship to Aerobic and Anaerobic Performance

    Directory of Open Access Journals (Sweden)

    Yoav Meckel

    2013-12-01

    Full Text Available The purpose of this study was to determine indices of swimming repeated sprint ability (RSA in 19 elite water polo players compared to 16 elite swimmers during a repeated sprint swimming test (RST, and to examine the relationships between these indices and aerobic and anaerobic performance capabilities in both groups. Indices of RSA were determined by the ideal sprint time (IS, the total sprint time (TS, and the performance decrement (PD recorded during an 8 x 15-m swimming RST. Single long - (800-m and short-(25-m distance swim tests were used to determined indices of aerobic and anaerobic swimming capabilities, respectively. The water polo players exhibited lower RSA swimming indices, as well as lower scores in the single short and long swim distances, compared to the swimmers. Significant relationships were found between the 25- m swim results and the IS and the TS, but not the PD of both the swimmers and the water polo players. No significant relationships were found between the 800-m swim results and any of the RSA indices in either the swimmers or the water polo players. No significant relationships were found between the 25-m and the 800-m swim results in either the swimmers or the water polo players. The results indicate that swimmers posses better RSA as well as higher anaerobic and aerobic capabilities, as reflected by the single short- and long-distance swim tests, compared to water polo players. The results also indicate that, as for running and cycling, repeated sprint swim performance is strongly related to single sprint performance.

  1. Investigation of decolorization of textile wastewater in an anaerobic/aerobic biological activated carbon system (A/A BAC).

    Science.gov (United States)

    Pasukphun, N; Vinitnantharat, S; Gheewala, S

    2010-04-01

    The aim of this study is to investigate the decolorization in anaerobic/aerobic biological activated carbon (A/A BAC) system. The experiment was divided into 2 stages; stage I is batch test for preliminary study of dye removal equilibrium time. The preliminary experiment (stage I) provided the optimal data for experimental design of A/A BAC system in SBR (stage II). Stage II is A/A BAC system imitated Sequencing Batch Reactor (SBR) which consist of 5 main periods; fill, react, settle, draw and idle. React period include anaerobic phase followed by aerobic phase. The BAC main media; Granular Activated Carbon (GAC), Mixed Cultures (MC) and Biological Activated Carbon (BAC) were used for dye and organic substances removal in three different solutions; Desizing Agent Solution (DAS), dye Solution (DS) and Synthetic Textile Wastewater (STW). Results indicate that GAC adsorption plays role in dye removal followed by BAC and MC activities, respectively. In the presence desizing agent, decolorization by MC was improved because desizing agent acts as co-substrates for microorganisms. It was found that 50% of dye removal efficiency was achieved in Fill period by MC. GC/MS analysis was used to identify dye intermediate from decolorization. Dye intermediate containing amine group was found in the solution and on BAC surfaces. The results demonstrated that combination of MC and BAC in the system promotes decolorization and dye intermediate removal. In order to improve dye removal efficiency in an A/A BAC system, replacement of virgin GAC, sufficient co-substrates supply and the appropriate anaerobic: aerobic period should be considered.

  2. An alphaproteobacterium capable of both aerobic and anaerobic anoxygenic photosynthesis but incapable of photoautotrophy: Charonomicrobium ambiphototrophicum, gen. nov., sp. nov.

    Science.gov (United States)

    Csotonyi, J T; Stackebrandt, E; Swiderski, J; Schumann, P; Yurkov, V

    2011-03-01

    A facultatively aerobic deep brown coccoid to ovoid bacterium, strain EG17(T), was isolated from a saline effluent stream in the NaCl-dominated brine spring system known as East German Creek in the province of Manitoba, Canada. The strain produced BChl a incorporated into a functional reaction center and two light-harvesting complexes with absorption peaks at 802, 850, and 879 nm. EG17(T) is the first reported anoxygenic phototroph capable of photoheterotrophic growth under both oxic and anoxic conditions. It yielded proportionally the greatest aerobic photosynthetic biomass under oligotrophic conditions. The results of 16S rRNA gene sequence comparisons revealed that EG17(T) was related most closely to the aerobic anoxygenic phototrophs Roseibacterium elongatum (98.3%) and quite distantly to both Dinoroseobacter shibae (95.2%) and Roseicyclus mahoneyensis (94.7%). The DNA G + C content was 65.6 mol%. On the basis of the unique dual aerobic/anaerobic photosynthetic capability, the distinctive spectrophotometric absorption of the photosynthetic apparatus, diagnostic physiological and biochemical traits, and the moderate phylogenetic separation between EG17(T) and its nearest relatives, it is concluded that this microorganism should be classified as a novel genus and species, Charonomicrobium ambiphototrophicum gen. nov., sp. nov., with EG17(T) as the type strain.

  3. The relationship of aerobic capacity, anaerobic peak power and experience to performance in CrossFit exercise

    OpenAIRE

    Bellar, D; Hatchett, A; Judge, LW; Breaux, ME; Marcus, L.

    2015-01-01

    CrossFit is becoming increasingly popular as a method to increase fitness and as a competitive sport in both the Unites States and Europe. However, little research on this mode of exercise has been performed to date. The purpose of the present investigation involving experienced CrossFit athletes and naïve healthy young men was to investigate the relationship of aerobic capacity and anaerobic power to performance in two representative CrossFit workouts: the first workout was 12 minutes in dur...

  4. Micro-aerobic, anaerobic and two-stage condition for ethanol production by Enterobacter aerogenes from biodiesel-derived crude glycerol

    DEFF Research Database (Denmark)

    Saisaard, Kanokrat; Angelidaki, Irini; Prasertsan, Poonsuk

    2011-01-01

    The microbial production of ethanol from biodiesel-derived crude glycerol by Enterobacter aerogenes TISTR1468, under micro-aerobic and anaerobic conditions, was investigated. The experimental results showed that micro-aerobic conditions were more favorable for cellular growth (4.0 g/L DCW), ethanol...... production (20.7 g/L) as well as the ethanol yield (0.47 g/g glycerol) than anaerobic conditions (1.2 g/L DCW, 6.3 g/L ethanol and 0.72 g/g glycerol, respectively). Crude glycerol (100 g/L) was consumed completely with the rate of 1.80 g/L/h. Two-stage fermentation (combination of micro-aerobic and anaerobic...

  5. Laboratory simulation of the successive aerobic and anaerobic degradation of oil products in oil-contaminated high-moor peat

    Science.gov (United States)

    Tolpeshta, I. I.; Trofimov, S. Ya.; Erkenova, M. I.; Sokolova, T. A.; Stepanov, A. L.; Lysak, L. V.; Lobanenkov, A. M.

    2015-03-01

    A model experiment has been performed on the successive aerobic and anaerobic degradation of oil products in samples of oil-contaminated peat sampled from a pine-subshrub-sphagnum bog near the Sutormin oilfield pipeline in the Yamal-Nenets autonomous district. During the incubation of oil-contaminated peat with lime and mineral fertilizers under complete flooding, favorable conditions are created for the aerobic oxidation of oil products at the beginning of the experiment and, as the redox potential decreases, for the anaerobic degradation of oil products conjugated with the reduction of N5+ and S+6 and methanogenesis. From the experimental data on the dynamics of the pH; Eh; and the NO{3/-}, NO{2/-}, and SO{4/2-} concentrations in the liquid phase of the samples, it has been found that denitrifiers significantly contributed to the biodegradation of oil products under the experimental conditions. After the end of the experiment, the content of oil products in the contaminated samples decreased by 21-26%.

  6. Chemical Composition, Cytotoxic and Antimicrobial Activity of Essential Oils from Cassia bakeriana Craib. against Aerobic and Anaerobic Oral Pathogens

    Directory of Open Access Journals (Sweden)

    Cláudio V. da Silva

    2013-04-01

    Full Text Available The chemical composition of the essential oils from leaves, bark and wood of Cassia bakeriana Craib. was determined by gas chromatography (GC and gas chromatography–mass spectrometry (GC-MS. Alcohols, aldehydes and fatty acids were the major components in leaf and bark oil, while wood essential oil was rich in fatty acids. Terpenes such as linalool, (E-nerolidol and phytol were present in low concentrations. The antimicrobial activity against aerobic and anaerobic oral bacteria was evaluated using the microdilution method, as was the cell viability test carried out with Vero cells. The oils from leaves and bark showed high antimicrobial activity, with minimum inhibitory concentrations between 62.5 and 125 µg·mL−1 for most of the tested bacteria, including Streptococcus mutans, the main etiological agent of dental caries. Leaves oil displayed the lowest cytotoxic effect (EC50 of 153 µg·mL−1, while wood oil exhibited the highest toxicity to Vero cells. C. bakeriana oils are thus a source of biologically active compounds against aerobic and anaerobic oral microorganisms. This study is the first report on the chemical composition, antimicrobial activity and cytotoxicity of C. bakeriana.

  7. Paragonimus westermani possesses aerobic and anaerobic mitochondria in different tissues, adapting to fluctuating oxygen tension in microaerobic habitats.

    Science.gov (United States)

    Takamiya, Shinzaburo; Fukuda, Koich; Nakamura, Takeshi; Aoki, Takashi; Sugiyama, Hiromu

    2010-12-01

    We previously showed that adult Paragonimus westermani, the causative agent of paragonimiasis and whose habitat is the host lung, possesses both aerobic and anaerobic respiratory chains, i.e., cyanide-sensitive succinate oxidase and NADH-fumarate reductase systems, in isolated mitochondria (Takamiya et al., 1994). This finding raises the intriguing question as to whether adult Paragonimus worms possess two different populations of mitochondria, one having an aerobic succinate oxidase system and the other an anaerobic fumarate reductase system, or whether the worms possess a single population of mitochondria possessing both respiratory chains (i.e., mixed-functional mitochondria). Staining of trematode tissues for cytochrome c oxidase activity showed three types of mitochondrial populations: small, strongly stained mitochondria with many cristae, localised in the tegument and tegumental cells; and two larger parenchymal cell mitochondria, one with developed cristae and the other with few cristae. The tegumental and parenchymal mitochondria could be separated by isopycnic density-gradient centrifugation and showed different morphological characteristics and respiratory activities, with low-density tegumental mitochondria having cytochrome c oxidase activity and high-density parenchymal mitochondria having fumarate reductase activity. These results indicate that Paragonimus worms possess three different populations of mitochondria, which are distributed throughout trematode tissues and function facultatively, rather than having mixed-functional mitochondria.

  8. Fact-finding survey of actual garbage discharged from dormitory and its biological anaerobic-aerobic treatment.

    Science.gov (United States)

    Imai, T; Ukita, M; Sekine, M; Fukagawa, M; Nakanishi, H

    2000-01-01

    The objective of this study is to find a possibility of complete treatment of garbage and resource recovery (production of methane from available utility of carbon resource in garbage) by biological treatment process. As the first step, a fact-finding survey of actual garbage discharged from the dormitory of the Ube National College of Technology (equivalent to 300 population) was carried out. Second, the combined biological anaerobic-aerobic treatment, i.e. combination of upflow anaerobic sludge blanket (UASB) process and aerobic membrane bioreactor (AMB) process, was applied to the garbage treatment. The applicability and efficiency of this system were investigated in this study. The survey results showed that the composition and quantity of garbage from a student dormitory changed slightly during a week due to the change of the menu, however, they remained almost unchanged during the entire experimental period. The experimental results showed high biodegradability of the garbage, and demonstrated its suitability for methane production. The soluble nitrogen removal was high: over 97%. No excess sludge was wasted from the system. A high treatment efficiency of simultaneous organic carbon and nitrogen was obtained. The possibility of complete treatment of garbage with this process has been positively demonstrated by this study.

  9. Chemical composition, cytotoxic and antimicrobial activity of essential oils from Cassia bakeriana Craib. against aerobic and anaerobic oral pathogens.

    Science.gov (United States)

    Cunha, Luís C S; de Morais, Sérgio A L; Martins, Carlos H G; Martins, Mário M; Chang, Roberto; de Aquino, Francisco J T; de Oliveira, Alberto; Moraes, Thaís da S; Machado, Fabrício C; da Silva, Cláudio V; do Nascimento, Evandro A

    2013-04-18

    The chemical composition of the essential oils from leaves, bark and wood of Cassia bakeriana Craib. was determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Alcohols, aldehydes and fatty acids were the major components in leaf and bark oil, while wood essential oil was rich in fatty acids. Terpenes such as linalool, (E)-nerolidol and phytol were present in low concentrations. The antimicrobial activity against aerobic and anaerobic oral bacteria was evaluated using the microdilution method, as was the cell viability test carried out with Vero cells. The oils from leaves and bark showed high antimicrobial activity, with minimum inhibitory concentrations between 62.5 and 125 µg·mL⁻¹ for most of the tested bacteria, including Streptococcus mutans, the main etiological agent of dental caries. Leaves oil displayed the lowest cytotoxic effect (EC₅₀ of 153 µg·mL⁻¹), while wood oil exhibited the highest toxicity to Vero cells. C. bakeriana oils are thus a source of biologically active compounds against aerobic and anaerobic oral microorganisms. This study is the first report on the chemical composition, antimicrobial activity and cytotoxicity of C. bakeriana.

  10. Prediction of aerobic and anaerobic capacities of elite cyclists from changes in lactate during isocapnic buffering phase.

    Science.gov (United States)

    Hasanli, Mohsen; Nikooie, Rohollah; Aveseh, Malihe; Mohammad, Fashi

    2015-02-01

    This study predicted aerobic and anaerobic capacities using relative changes of arterial blood lactate during the isocapnic buffering phase (relative [La]ISBP). Fourteen male professional cyclists (sprint-trained [n = 6] and endurance [n = 8]) performed 2 exercise sessions to exhaustion on a cycle ergometer; 1 incremental standard test to determine the isocapnic buffering phase, buffering capacities, and relative [La]ISBP and 1 supramaximal exercise test to determine maximal accumulated oxygen deficit (MAOD). The time between Lactate threshold (LT) and respiratory compensatory threshold (RCT) was considered to be the isocapnic buffering phase. Total buffering capacity was calculated as Δ[La]·ΔpH. Bicarbonate buffering was calculated as Δ[HCO3]·ΔpH, and the difference between -Δ[La]·ΔpH and Δ[HCO3]·ΔpH was considered as nonbicarbonate buffering. The lactate concentration for LT (p ≤ 0.05) and RCT (p ≤ 0.05), and relative [La]ISBP (p Equation is included in full-text article.)(r = -0.71, p ≤ 0.05) and MAOD (r = 0.73, p Equation is included in full-text article.)and LT is recommended for better evaluation of performance of athletes who show nearly equal contributions from the aerobic and anaerobic energy systems during exercise.

  11. Treatment of petroleum refinery wastewater using a sequential anaerobic-aerobic moving-bed biofilm reactor system based on suspended ceramsite.

    Science.gov (United States)

    Lu, Mang; Gu, Li-Peng; Xu, Wen-Hao

    2013-01-01

    In this study, a novel suspended ceramsite was prepared, which has high strength, optimum density (close to water), and high porosity. The ceramsite was used to feed a moving-bed biofilm reactor (MBBR) system with an anaerobic-aerobic (A/O) arrangement to treat petroleum refinery wastewater for simultaneous removal of chemical oxygen demand (COD) and ammonium. The hydraulic retention time (HRT) of the anaerobic-aerobic MBBR system was varied from 72 to 18 h. The anaerobic-aerobic system had a strong tolerance to shock loading. Compared with the professional emission standard of China, the effluent concentrations of COD and NH3-N in the system could satisfy grade I at HRTs of 72 and 36 h, and grade II at HRT of 18 h. The average sludge yield of the anaerobic reactor was estimated to be 0.0575 g suspended solid/g CODremoved. This work demonstrated that the anaerobic-aerobic MBBR system using the suspended ceramsite as bio-carrier could be applied to achieving high wastewater treatment efficiency.

  12. Strain-resolved microbial community proteomics reveals simultaneous aerobic and anaerobic function during gastrointestinal tract colonization of a preterm infant

    Directory of Open Access Journals (Sweden)

    Brandon eBrooks

    2015-07-01

    Full Text Available While there has been growing interest in the gut microbiome in recent years, it remains unclear whether closely related species and strains have similar or distinct functional roles and if organisms capable of both aerobic and anaerobic growth do so simultaneously. To investigate these questions, we implemented a high-throughput mass spectrometry-based proteomics approach to identify proteins in fecal samples collected on days of life 13-21 from an infant born at 28 weeks gestation. No prior studies have coupled strain-resolved community metagenomics to proteomics for such a purpose. Sequences were manually curated to resolve the genomes of two strains of Citrobacter that were present during the later stage of colonization. Proteome extracts from fecal samples were processed via a nano-2D-LC-MS/MS and peptides were identified based on information predicted from the genome sequences for the dominant organisms, Serratia and the two Citrobacter strains. These organisms are facultative anaerobes, and proteomic information indicates the utilization of both aerobic and anaerobic metabolisms throughout the time series. This may indicate growth in distinct niches within the gastrointestinal tract. We uncovered differences in the physiology of coexisting Citrobacter strains, including differences in motility and chemotaxis functions. Additionally, for both Citrobacter strains we resolved a community-essential role in vitamin metabolism and a predominant role in propionate production. Finally, in this case study we detected differences between genome abundance and activity levels for the dominant populations. This underlines the value in layering proteomic information over genetic potential.

  13. Feed restriction and a diet's caloric value: The influence on the aerobic and anaerobic capacity of rats

    Directory of Open Access Journals (Sweden)

    de Moura Leandro

    2012-03-01

    Full Text Available Abstract Background The influence of feed restriction and different diet's caloric value on the aerobic and anaerobic capacity is unclear in the literature. Thus, the objectives of this study were to determine the possible influences of two diets with different caloric values and the influence of feed restriction on the aerobic (anaerobic threshold: AT and anaerobic (time to exhaustion: Tlim variables measured by a lactate minimum test (LM in rats. Methods We used 40 adult Wistar rats. The animals were divided into four groups: ad libitum commercial Purina® diet (3028.0 Kcal/kg (ALP, restricted commercial Purina® diet (RAP, ad libitum semi-purified AIN-93 diet (3802.7 Kcal/kg (ALD and restricted semi-purified AIN-93 diet (RAD. The animals performed LM at the end of the experiment, 48 h before euthanasia. Comparisons between groups were performed by analysis of variance (p Results At the end of the experiment, the weights of the rats in the groups with the restricted diets were significantly lower than those in the groups with ad libitum diet intakes. In addition, the ALD group had higher amounts of adipose tissue. With respect to energetic substrates, the groups subjected to diet restriction had significantly higher levels of liver and muscle glycogen. There were no differences between the groups with respect to AT; however, the ALD group had lower lactatemia at the AT intensity and higher Tlim than the other groups. Conclusions We conclude that dietary restriction induces changes in energetic substrates and that ad libitum intake of a semi-purified AIN-93 diet results in an increase in adipose tissue, likely reducing the density of the animals in water and favouring their performance during the swimming exercises.

  14. Evaluation of In-situ Sludge Reduction and Enhanced Nutrient Removal in an Integrated Repeatedly Coupling Aerobic and Anaerobic and Oxic-setting-anaerobic System

    Institute of Scientific and Technical Information of China (English)

    Shanshan Yang; Wanqian Guo; Qinglian Wu; Haichao Luo; Simai Peng; Heshan Zheng; Xiaochi Feng; Nanqi Ren∗

    2015-01-01

    Aiming to achieve simultaneous good performances of in⁃situ sludge reduction and effluent quality, an integrated repeatedly coupling aerobic and anaerobic and oxic⁃setting⁃anaerobic system ( rCAA+OSA ) is developed to reduce sludge production and enhance nutrient removal. Considering the mechanism of in⁃situ sludge reduction in this rCAA+OSA system, the combined effect of energy uncoupling metabolism and sludge cryptic growth maybe attributed to the higher reduction of biomass. Results show that the maximal sludge reduction in this rCAA+OSA system is obtained when the hydraulic retention time ( HRT ) is controlled at 6�5 h, which an increase in 16�67% reduction in excess sludge is achieved compared with OSA system ( HRT of 6�5 h) . When compared the performances of effluent qualities, the enhanced nutrient removal efficiencies also can be observed in this rCAA+OSA system. Three⁃dimensional excitation emission matrix ( 3D⁃EEM ) fluorescence spectroscopy is applied to characterize the effluent organic matters ( EfOM) under different HRTs in the OSA and the rCAA+OSA systems. Analyses of 3D⁃EEM spectra show that more refractory humic⁃like and fulvic⁃like components are observed in the effluent of the OSA system. On the basis of these results, simultaneous enhanced in⁃situ sludge reduction and improved nutrient removal can be obtained in the rCAA+OSA systems.

  15. Post-treatment of anaerobically degraded azo dye Acid Red 18 using aerobic moving bed biofilm process: enhanced removal of aromatic amines.

    Science.gov (United States)

    Koupaie, E Hosseini; Moghaddam, M R Alavi; Hashemi, S H

    2011-11-15

    The application of aerobic moving bed biofilm process as post-treatment of anaerobically degraded azo dye Acid Red 18 was investigated in this study. The main objective of this work was to enhance removal of anaerobically formed the dye aromatic metabolites. Three separate sequential treatment systems were operated with different initial dye concentrations of 100, 500 and 1000 mg/L. Each treatment system consisted of an anaerobic sequencing batch reactor (An-SBR) followed by an aerobic moving bed sequencing batch biofilm reactor (MB-SBBR). Up to 98% of the dye decolorization and more than 80% of the COD removal occurred anaerobically. The obtained results suggested no significant difference in COD removal as well as the dye decolorization efficiency using three An-SBRs receiving different initial dye concentrations. Monitoring the dye metabolites through HPLC suggested that more than 80% of anaerobically formed 1-naphthylamine-4-sulfonate was completely removed in the aerobic biofilm reactors. Based on COD analysis results, at least 65-72% of the dye total metabolites were mineralized during the applied treatment systems. According to the measured biofilm mass and also based on respiration-inhibition test results, increasing the initial dye concentration inhibited the growth and final mass of the attached-growth biofilm in MB-SBBRs.

  16. Factors affecting biological denitrifying dephosphatation in anaerobic/anoxic/aerobic sequencing batch reactor

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This study was conducted to verify and discuss the denitrifying dephosphatation under different levels of nitrate concentration and retention time of anoxic/aerobic process in a Sequencing Batch Reactor ( SBR ).The results of tests demonstrated that there were two kinds of phosphorus-accumulating organisms (PAOs) in the biological excess phosphorus removal (BEPR) system. One was non-DNPAOs that could only use oxygen as terminal electron acceptors, the other was denitrifying PAOs (DNPAOs) that could use both nitrate and oxygen as terminal electron acceptors. Phosphorus uptake efficiency could be attained under anoxic period ranging from 28.7%-96.7% in an anacrobic/anoxic/aerobic system. Experimental results showed that nitrate concentration and retention time of anoxic/aerobic process were the key factors affecting the course of denitrifying dephosphatation.

  17. Partition of aerobic and anaerobic swimming costs and their correlation to tail-beat frequency and burst activity in Sparus aurata

    DEFF Research Database (Denmark)

    Steffensen, John Fleng

    2012-01-01

    until fatigue at 10°C. The anaerobic swimming cost was measured as the excess postexercise oxygen consumption (EPOC) following each swimming speed. To determine tail-beat frequency, amplitude and burst and coast behaviour, the peduncle position was determined at 25 s·' by video tracking. The data showed......, and resulted in a total anaerobic capacity of 170 mg O2 kg·'. Normalized tail-beat amplitude and frequency both predicted the swimming speed but only tail-beat frequency was able to predict the aerobic swimming cost. The change to burst and coast swimming was correlated to the first measurements of EPOC......Soc for experimental Biol Annual Meeting - Salzburg 2012 Bjørn Tirsgaard (University of Copenhagen, Denmark) and John Fleng Steffensen (University of Copenhagen, Denmark) Aerobic and anaerobic oxygen consumption was measured for 13 Sparus aurata swimming at incremental increasing swimming speeds...

  18. Advanced removal of organic and nitrogen from ammonium-rich landfill leachate using an anaerobic-aerobic system☆

    Institute of Scientific and Technical Information of China (English)

    Hongwei Sun; Huanan Zhao; Baoxia Bai; Yuying Chen; Qing Yang; Yongzhen Peng

    2015-01-01

    A novel system coupling an up-flow anaerobic sludge blanket (UASB) and sequencing batch reactor (SBR) was introduced to achieve advanced removal of organic and nitrogen from ammonium-rich landfill leachate. UASB could remove 88.1%of the influent COD at a volumetric loading rate of 6.8 kg COD·m−3·d−1. Nitritation–denitritation was responsible for removing 99.8%of NH4+-N and 25%of total nitrogen in the SBR under alternating aerobic/anoxic modes. Simultaneous denitritation and methanogenesis in the UASB enhanced COD and TN removal, and replenished alkalinity consumed in nitritation. For the activated sludge of SBR, ammonia oxidizing bacteria were preponderant in nitrifying population, indicated by fluorescence in situ hybridization (FISH) anal-ysis. The Monod equation is appropriate to describe the kinetic behavior of heterotrophic denitrifying bacteria, with its kinetic parameters determined from batch experiments.

  19. Development and evaluation of a radial anaerobic/aerobic reactor treating organic matter and nitrogen in sewage

    Directory of Open Access Journals (Sweden)

    L. H. P. Garbossa

    2005-12-01

    Full Text Available The design and performance of a radial anaerobic/aerobic immobilized biomass (RAAIB reactor operating to remove organic matter, solids and nitrogen from sewage are discussed. The bench-scale RAAIB was divided into five concentric chambers. The second and fourth chambers were packed with polyurethane foam matrices. The performance of the reactor in removing organic matter and producing nitrified effluent was good, and its configuration favored the transfer of oxygen to the liquid mass due to its characteristics and the fixed polyurethane foam bed arrangement in concentric chambers. Partial denitrification of the liquid also took place in the RAAIB. The reactor achieved an organic matter removal efficiency of 84%, expressed as chemical oxygen demand (COD, and a total Kjeldahl nitrogen (TKN removal efficiency of 96%. Average COD, nitrite and nitrate values for the final effluent were 54 mg.L-1, 0.3 mg.L-1 and 22.1 mg.L-1, respectively.

  20. Effects of biodegradation of mecoprop, dichlorprop and bentazone by changing the redox conditions from anaerobic to aerobic in sandy aquifer

    DEFF Research Database (Denmark)

    Levi, Suzi; Bjerg, Poul Løgstrup; Albrechtsen, Hans-Jørgen

    2011-01-01

    in combination with geological variations may affect microbial degradation processes by mixing in even low oxygen concentrations and create steep gradients and drastic redox zones in aquifers. The aim of the current study was to investigate the stimulation of pesticides biodegradation by adding oxygen...... knowledge, this is the first observation of bentazone degradation with aquifer. Optimization of redox conditions from anaerobic to aerobic by adding oxygen stimulated the biodegradation of three pesticides.......Pesticides and metabolites are detected in an increasing number of aquifers resulting in closure of many drinking water wells. The natural redox conditions of aquifers are considered to be one of the important factors governing the pesticides degradation in groundwater. Water abstraction strategy...

  1. In situ measurements of soil solution to assess the influence of aerobic/anaerobic conditions on metal mobility

    Science.gov (United States)

    Cary, L.; Trolard, F.; Bourrie, G.

    2003-04-01

    Submerged soils are submitted to fast variations of aeration and aerobic-anaerobic conditions. Especially, paddy soils show hydromorphic phenomena on surface - induced by flooding - and in shallow depth in the soil. In Camargue (France), there occurs natural and intensively rice cultivated soils with an aquifer at less than 2 m deep. The aim is to observe and modelize the effects of aerobic-anaerobic variations on the mobility of metals in soils (Pb, Cu, Zn, Fe, Mn and Al). These are linked to oxygen inputs in the soil, due to aeration or oxygenation (Mediterranean storms), O_2 consumption and oxido-reduction processes. While Al, Fe and Mn are mainly inherited, Cu, Zn and Pb are mostly brought by anthropogenic inputs and/or atmospheric deposits. In order to monitor fast variations, we have installed instruments to acquire continuous and in situ measures since summer 2002. We used a multiparametric probe designed for oceanic measurements in seawater. The probe was adapted to monitor in situ pH, oxido-reduction potential, dissolved oxygen, electric conductivity, temperature and pressure in soil solution at 1.1 m depth every hour. It does not require maintenance for months. We added tubing to sample the soil solution in the measuring cell, free from atmosphere and light. Fe(II), nitrogen and sulphides are immediately analysed with a portable spectrophotometer. The results show that low frequency events, such as storms, influence strongly the chemical composition of soil solution at depth, through sharp variations of pH, Eh, Al, Fe(II) and Mn concentrations. The variations of heavy metals are best explained by considering the dynamics of these latter metals, which suggests that soil Al, Fe and Mn oxides act both as sources and as sinks for the former metals.

  2. Two-Dimensional Stable Isotope Fractionation During Aerobic and Anaerobic Alkane Biodegradation and Implications for the Field

    Science.gov (United States)

    El Morris, Brandon; Suflita, Joseph M.; Richnow, Hans-Hermann

    2010-05-01

    Quantitatively, n-alkanes comprise a major portion of most crude oils. In petroliferous formations, it may be possible to relate the loss of these compounds to the levels of biodegradation occurring in situ [1]. Moreover, it is important to develop indicators of alkane degradation that may be used to monitor bioremediation of hydrocarbon-impacted environments. Desulfoglaeba alkanexedens and Pseudomonas putida GPo1 were used to determine if carbon and hydrogen stable isotope fractionation could differentiate between n-alkane degradation under anaerobic and aerobic conditions, respectively in the context of the Rayleigh equation model [2]. Bacterial cultures were sacrificed by acidification and headspace samples were analyzed for stable isotope composition using gas chromatography-isotope ratio mass spectrometry. Carbon enrichment factors (bulk) for anaerobic and aerobic biodegradation of hexane were -5.52 ± 0.2‰ and -4.34 ± 0.3‰, respectively. Hydrogen enrichment during hexane degradation was -43.14 ± 6.32‰ under sulfate-reducing conditions, and was too low for quantification during aerobiosis. Collectively, this indicates that the correlation between carbon and hydrogen stable isotope fractionation (may be used to help elucidate in situ microbial processes in oil reservoirs, and during intrinsic as well as engineered remediation efforts. References 1. Asif, M.; Grice, K.; Fazeelat, T., Assessment of petroleum biodegradation using stable hydrogen isotopes of individual saturated hydrocarbon and polycyclic aromatic hydrocarbon distributions in oils from the Upper Indus Basin, Pakistan. Organic Geochemistry 2009, 40, (3), 301-311. 2. Fischer, A.; Herklotz, I.; Herrmann, S.; Thullner, M.; Weelink, S. A. B.; Stams, A., J. M.; Schloemann, M.; Richnow, H.-H.; Vogt, C., Combined carbon and hydrogen isotope fractionation investigations for elucidating benzene biodegradation pathways. Environ. Sci. Technol. 2008, 42, 4356-4363.

  3. Exploration and comparison of inborn capacity of aerobic and anaerobic metabolisms of Saccharomyces cerevisiae for microbial electrical current production

    Science.gov (United States)

    Mao, Longfei; Verwoerd, Wynand S

    2013-01-01

    Saccharomyces cerevisiae possesses numerous advantageous biological features, such as being robust, easily handled, mostly non-pathogenic and having high catabolic rates, etc., which can be considered as merits for being used as a promising biocatalyst in microbial fuel cells (MFCs) for electricity generation. Previous studies have developed efficient MFC configurations to convert metabolic electron shuttles, such as cytoplasmic NADH, into usable electric current. However, no studies have elucidated the maximum potential of S. cerevisiae for current output and the underlying metabolic pathways, resulting from the interaction of thousands of reactions inside the cell during MFC operation. To address these two key issues, this study used in silico metabolic engineering techniques, flux balance analysis (FBA), and flux variability analysis with target flux minimization (FATMIN), to model the metabolic perturbation of S. cerevisiae under the MFC-energy extraction. The FBA results showed that, in the cytoplasmic NADH-dependent mediated electron transfer (MET) mode, S. cerevisiae had a potential to produce currents at up to 5.781 A/gDW for the anaerobic and 6.193 A/gDW for the aerobic environments. The FATMIN results showed that the aerobic and anaerobic metabolisms are resilient, relying on six and five contributing reactions respectively for high current production. Two reactions, catalyzed by glutamate dehydrogenase (NAD) (EC 1.4.1.3) and methylene tetrahydrofolate dehydrogenase (NAD) (EC 1.5.1.5), were shared in both current-production modes and contributed to over 80% of the identified maximum current outputs. It is also shown that the NADH regeneration was much less energy costly than biomass production rate. Taken together, our finding suggests that S. cerevisiae should receive more research effort for MFC electricity production. PMID:23969939

  4. The Effect of Colostrum along with Aerobic and Anaerobic Exercise on Lipid Peroxidation and Total Antioxidant Capacity of Male Wistar Rats

    Directory of Open Access Journals (Sweden)

    M Mogharnasi

    2016-06-01

    Full Text Available Background & aim: The consumption of food supplements in order to eliminate oxidative damages induced by exercise are common among athletes. Previous studies have shown that bovine colostrum has antioxidant properties, but no study has ever been done to evaluate its effectiveness on Oxidative stress and antioxidant capacity. The aim of study was to investigate the effects of bovine colostrum along with aerobic and anaerobic exercise on Lipid peroxidation and antioxidant capacity in male Wistar rats.   Methods: In the present experimental study, 48 male Wistar rats were randomly divided into six groups (control, colostrum supplement, aerobic exercise, anaerobic exercise, colostrum supplements and aerobic exercise, colostrum supplements and anaerobic exercise. Colostrum group received daily for ten weeks dosing 300 mg /kg bovine colostrum powder orally. Exercising groups worked out three times a week for a period of 10 weeks on a custom-made treadmill for rodents. Blood samples were taken before and 24 hours after the last exercise session on an empty stomach. Data were analyzed using Kolmogorov-Smirnov tests, One Way ANOVA and post hoc Tukey at α<0.05.   Results: The plasma levels of oxidative stress index (MDA in all groups except colostrum supplement and anaerobic exercise compared with the control group was significantly reduced (p<0.05. The antioxidant capacity in all groups except anaerobic exercise group compared with the control group was significant increased (p<0.05.   Conclusions: The results indicated that colostrum supplementation with ten weeks of aerobic exercise had better effect on the control of oxidative stress and antioxidant capacity compared to anaerobic exercise.    

  5. Fate of oestrogens during anaerobic blackwater treatment with micro-aerobic post-treatment

    NARCIS (Netherlands)

    Mes, de T.Z.D.; Kujawa-Roeleveld, K.; Zeeman, G.; Lettinga, G.

    2007-01-01

    The fate of oestrone (E1), 17b-oestradiol (E2) and 17a-ethynyloestradiol (EE2) was investigated in a concentrated blackwater treatment system consisting of an UASB septic tank, with micro-aerobic post-treatment. In UASB septic tank effluent a (natural) total concentration of 4.02 mg/L E1 and 18.69 m

  6. Kinetic analysis of phenol, thiocyanate and ammonia-nitrogen removals in an anaerobic-anoxic-aerobic moving bed bioreactor system

    Energy Technology Data Exchange (ETDEWEB)

    Sahariah, Biju Prava [Centre for the Environment, Indian Institute of Technology Guwahati, Assam 781039 (India); Chakraborty, Saswati, E-mail: chakraborty_saswati@hotmail.com [Department of Civil Engineering, Indian Institute of Technology Guwahati, Assam 781039 (India)

    2011-06-15

    A simulated wastewater containing phenol (2500 mg/L), thiocyanate and ammonia-nitrogen (500 mg/L) was treated in an anaerobic (R1)-anoxic (R2)-aerobic (R3) moving bed biofilm reactor system at different hydraulic retention time (HRT) intervals (total HRT 3-8 days, R1: 1.5-4 days; R2: 0.75-2 days and R3: 0.75-2 days) and feed thiocyanate (SCN{sup -}) concentrations (110-600 mg/L) to determine substrate removal kinetics. In R1, phenol and COD reduction and specific methanogenic activity were inhibited due to the increase of SCN{sup -} in feed. Bhatia et al. model having inbuilt provision of process inhibition described the kinetics of COD and phenol utilization with maximum utilization rates of 0.398 day{sup -1} and 0.486 day{sup -1}, respectively. In R2 and R3 modified Stover-Kincannon model was suitable to describe substrate utilization. In R2 respective maximum SCN{sup -}, phenol, COD and NO{sub 3}{sup -}N utilization rates were 0.23, 5.28, 37.7 and 11.82 g/L day, respectively. In aerobic reactor R3, COD, SCN{sup -} and NH{sub 4}{sup +}-N removal rates were, respectively, 10.53, 1.89, and 2.17 g/L day. The minimum total HRT of three-stage system was recommended as 4 days.

  7. Biosynthesis of pyridoxine in Saccharomyces cerevisiae--origin of the pyridoxine nitrogen atom differs under anaerobic and aerobic conditions.

    Science.gov (United States)

    Ishida, Shiho; Yamada, Kazuko

    2002-12-01

    The amide nitrogen atom of glutamine is incorporated into pyridoxine in four eukaryotes (i.e., Emericella nidulans, Mucor racemosus, Neurospora crassa and Saccharomyces cerevisiae) and two prokaryotes (i.e., Staphylococcus aureus and Bacillus subtilis). However, in the prokaryotes Pseudomonas putida, Enterobacter aerogenes and Escherichia coli, it is the nitrogen atom of glutamate that is incorporated into pyridoxine (J Nutr Sci Vitaminol (2000) 46, 55-57). As these results were from experiments conducted under aerobic conditions, we investigated the biosynthesis of pyridoxine on S. cerevisiae under anaerobic conditions. The results showed that [amide-15N]L-glutamine was not incorporated into pyridoxine, unlike the results for aerobic conditions. The incorporation of [15N]ammonium salts into pyridoxine was not inhibited in the presence of casamino acids and tryptophan. The results showed that the nitrogen atoms of amino acids are not used for the biosynthesis of pyridoxine. The incorporation of 15N into pyridoxine was inhibited in the presence of adenine, but not in that of hypoxanthine. Thus, the nitrogen atom of pyridoxine may be from the amino group attached to the C-6 of adenine.

  8. The treatment of cheese whey wastewater by sequential anaerobic and aerobic steps in a single digester at pilot scale.

    Science.gov (United States)

    Frigon, J-C; Breton, J; Bruneau, T; Moletta, R; Guiot, S R

    2009-09-01

    The treatment of reconstituted whey wastewater was performed in a 400 L digester at 20 degrees C, with an anaerobic digestion step, followed by a step of aerobic treatment at low oxygen concentration in the same digester. In a first set of 48 cycles, total cycle time (T(C)) of 2, 3 and 4 days were tested at varying organic loading rates (OLR). The COD removal reached 89+/-4, 97+/-3 and 98+/-2% at T(C) of 2, 3 and 4 days and OLR of 0.56, 1.04 and 0.78 g COD L(-1) d(-1), respectively. The activity of the biomass decreased for the methanogenic population, while increasing by 400% for the acidogens, demonstrating a displacement in the predominant trophic group in the biomass bed. A second set of 16 cycles was performed with higher soluble oxygen concentration in the bulk liquid (0.5 mg L(-1)) during the aerobic treatment at a T(C) of 2 days and an OLR of 1.55 g COD L(-1) d(-1), with a soluble COD removal of 88+/-3%. The biomass specific activities showed a compartmentalization of the trophic group with methanogenic activity maintained in the biomass bed and a high acidogenic activity in the suspended flocs.

  9. Sorption and release of organics by primary, anaerobic, and aerobic activated sludge mixed with raw municipal wastewater.

    Science.gov (United States)

    Modin, Oskar; Saheb Alam, Soroush; Persson, Frank; Wilén, Britt-Marie

    2015-01-01

    New activated sludge processes that utilize sorption as a major mechanism for organics removal are being developed to maximize energy recovery from wastewater organics, or as enhanced primary treatment technologies. To model and optimize sorption-based activated sludge processes, further knowledge about sorption of organics onto sludge is needed. This study compared primary-, anaerobic-, and aerobic activated sludge as sorbents, determined sorption capacity and kinetics, and investigated some characteristics of the organics being sorbed. Batch sorption assays were carried out without aeration at a mixing velocity of 200 rpm. Only aerobic activated sludge showed net sorption of organics. Sorption of dissolved organics occurred by a near-instantaneous sorption event followed by a slower process that obeyed 1st order kinetics. Sorption of particulates also followed 1st order kinetics but there was no instantaneous sorption event; instead there was a release of particles upon mixing. The 5-min sorption capacity of activated sludge was 6.5±10.8 mg total organic carbon (TOC) per g volatile suspend solids (VSS) for particulate organics and 5.0±4.7 mgTOC/gVSS for dissolved organics. The observed instantaneous sorption appeared to be mainly due to organics larger than 20 kDa in size being sorbed, although molecules with a size of about 200 Da with strong UV absorbance at 215-230 nm were also rapidly removed.

  10. Grey water treatment in a series anaerobicAerobic system for irrigation

    NARCIS (Netherlands)

    Abu-Ghunmi, L.N.A.H.; Zeeman, G.; Fayyad, M.; Lier, van J.B.

    2010-01-01

    This study aims at treatment of grey water for irrigation, focusing on a treatment technology that is robust, simple to operate and with minimum energy consumption. The result is an optimized system consisting of an anaerobic unit operated in upflow mode, with a 1 day operational cycle, a constant e

  11. AEROBIC AND ANAEROBIC TREATMENT OF C.I. DISPERSE BLUE 79 - VOLUME II, APPENDICES

    Science.gov (United States)

    This study was conducted to determine the fate of C.I. Disperse Blue 79, one of the largest production volume dyes, and select biodegradation products in a conventionally operated activated sludge process and an anaerobic sludge digestion system. To achieve this objective, a pilo...

  12. AEROBIC AND ANAEROBIC TREATMENT OF C.I. DISPERSE BLUE 79 - VOLUME I

    Science.gov (United States)

    This study was conducted to determine the fate of C.I. Disperse Blue 79, one of the largest production volume dyes, and select biodegradation products in a conventionally operated activated sludge process and an anaerobic sludge digestion system. To achieve this objective, a pilo...

  13. Performance of Combined Anaerobic-Aerobic System in Removal of Nitrogen in the Treatment of Sanitary Sewage

    Directory of Open Access Journals (Sweden)

    Mário Luiz Rodrigues Foco

    2014-10-01

    Full Text Available This paper describes the evaluation of the performance of an anaerobic-aerobic system, consisting of anaerobic filter (AF followed by biological aerated filter (BAF, operated with and without recirculation of treated effluent to mitigate the adverse effects of organic concentrations on nitrification and promote the removal of Total Nitrogen in anoxic ambient. In the first two steps, the application of the organic loading rate (OLR on BAF ranged from 0.49 ± 0.27 and 0.31 ± 0.08 kg BOD m-3 d-1 due to the reduction of surface application rate of 60 to 44 m3 m-2 d-1. In the third stage, with recirculation, OLR and surface application rate were 0.29 ± 0.02 kg BOD m-3 d-1 and 65.8 m3 m-2 d-1, allowing the system to reduce 43 ± 10% of average initial concentration of 72 mg L-1 Total Nitrogen. The application of organic loading rates in BAS, more than 0.30 kg BOD m-3 d-1 inhibited nitrification due to competition for oxygen

  14. Visual Impairment does not Limit Training Effects in Development of Aerobic and Anaerobic Capacity in Tandem Cyclists

    Directory of Open Access Journals (Sweden)

    Malwina Kamelska Anna

    2015-12-01

    Full Text Available The study aimed to investigate the differences in the effects of 7-month training on aerobic and anaerobic capacity in tandem cycling athletes with and without visual impairment. In this study, Polish elite (n=13 and sub-elite (n=13 visually impaired (VI (n=13; 40.8 ±12.8 years and properly sighted (PS (n=13; 36.7 ±12.2 years tandem-cycling athletes participated voluntarily in 7-month routine training. The following pre-/post-training measurements were conducted on separate days: maximal oxygen uptake (VO2max was estimated with age correction using the Physical Working Capacity test on a bicycle ergometer according to the Astrand-Ryhming method. Maximal power output (Pmax was evaluated using the Quebec test on a bicycle ergometer. At baseline, VO2max (47.8 ±14.1 vs 42.0 ±8.3 ml/kg/min, respectively and Pmax (11.5 ±1.5 vs 11.5 ±1.0 W/kg did not differ significantly between PS and VI cyclists. However, differences in aerobic capacity were considered as clinically significant. Two-way ANOVA revealed that after 7 month training, there were statistically significant increases in VO2max (p=0.003 and Pmax (p=0.009 among VI (VO2max, +9.1%; Pmax, +6.3% and PS (VO2max, +9.1%; Pmax, +11.7% cyclists, however, no time x visual impairment interaction effect was found (VO2max, p=0.467; Pmax, p=0.364. After training, VO2max (p=0.03, but not Pmax (p=0.13, was significantly greater in elite compared to sub-elite tandem cyclists. VI and PS tandem cyclists showed similar rates of improvement in VO2max and Pmax after 7-month training. VO2max was a significant determinant of success in tandem cycling. This is one of the first studies providing reference values for aerobic and anaerobic capacity in visually impaired cyclists.

  15. The effects of cigarette smoking on aerobic and anaerobic capacity and heart rate variability among female university students

    Directory of Open Access Journals (Sweden)

    Lee CL

    2013-10-01

    Full Text Available Chia-Lun Lee,1 Wen-Dien Chang21Physical Education Section for General Education, National Sun Yat-sen University, Kaohsiung, Taiwan; 2Department of Sports Medicine, China Medical University, Taichung, TaiwanAim: In this study, the effects of cigarette smoking on maximal aerobic capacity, anaerobic capacity, and heart rate variability among female university students were investigated.Materials and methods: Twelve smokers and 21 nonsmokers participated in this study. All participants performed an intermittent sprint test (IST and a 20 m shuttle run test to measure their anaerobic capacity and maximal aerobic capacity. The IST was comprised of 6 × 10-second sprints with a 60-second active recovery between each sprint. Heart rate variability was recorded while the participants were in a supine position 20 minutes before and 30 minutes after the IST.Results: The total work, peak power, and heart rate of the smokers and nonsmokers did not differ significantly. However, the smokers’ average power declined significantly during sprints 4 to 6 (smokers versus nonsmokers, respectively: 95% confidence interval =6.2–7.2 joule/kg versus 6.8–7.6 joule/kg; P<0.05, and their fatigue index increased (smokers versus nonsmokers, respectively: 35.8% ± 2.3% versus 24.5% ± 1.76%; P<0.05 during the IST. The maximal oxygen uptake of nonsmokers was significantly higher than that of the smokers (P<0.05. The standard deviation of the normal to normal intervals and the root mean square successive difference did not differ significantly between nonsmokers and smokers. However, the nonsmokers exhibited a significantly higher normalized high frequency (HF, and significantly lower normalized low frequency (LF, LF/HF ratio, and natural logarithm of the LF/HF when compared with those of the smokers (P<0.05.Conclusion: Smoking may increase female smokers’ exercise fatigue and decrease their average performance during an IST, while reducing their maximal aerobic

  16. Bacterial degradation of benzoate: cross-regulation between aerobic and anaerobic pathways

    OpenAIRE

    2012-01-01

    We have studied for the first time the transcriptional regulatory circuit that controls the expression of the box genes encoding the aerobic hybrid pathway used to assimilate benzoate via coenzyme A (CoA) derivatives in bacteria. The promoters responsible for the expression of the box cluster in the β-proteobacterium Azoarcus sp., their cognate transcriptional repressor, the BoxR protein, and the inducer molecule (benzoyl-CoA) have been characterized. The BoxR protein shows a significant sequ...

  17. Regulation and Function of Versatile Aerobic and Anaerobic Respiratory Metabolism in Pseudomonas aeruginosa

    OpenAIRE

    Hiroyuki eArai

    2011-01-01

    Pseudomonas aeruginosa is a ubiquitously distributed opportunistic pathogen that inhabits soil and water as well as animal-, human-, and plant-host-associated environments. The ubiquity would be attributed to its very versatile energy metabolism. P. aeruginosa has a highly branched respiratory chain terminated by multiple terminal oxidases and denitrification enzymes. Five terminal oxidases for aerobic respiration have been identified in the P. aeruginosa cells. Three of them, the cbb 3-1 oxi...

  18. Effects of Packaging, Treatments, and Storage Conditions on the Survivability of Aerobes and Anaerobes in Vacuum Packaged ‘Wara’ a Soft White Cheese

    Directory of Open Access Journals (Sweden)

    Victoria O. Adetunji

    2011-08-01

    Full Text Available This study assessed total aerobes and anaerobes in vacuum packaged ‘wara’ a West African soft white cheese processed with lemon juice as coagulant. The cheese was treated with Carica papaya (Vcpc, Terminalia cattapa (Vtcc crude extracts, nisin (Vnc, and the combination of these three treatments (V+3 and stored at 15 and 28ºC for a three weeks storage period. The total aerobes and anaerobes were suppressed to an undetectable level (<1.00 logcfu/mL in Vcpc at 15ºC in the first week of storage. In Vtcc the total aerobes which was initially 6.24 logcfu/mL in the first week of storage was suppressed to 3.16 log cfu/mL by the 3rd week of storage. Inhibition of microbes was not apparent in Vnc and V+3. Significant differences occurred in the microbial count at the two storage temperatures and storage weeks (p<0.05. It can therefore be concluded from this work that Vacuum packaging and addition of crude extracts (Carica papaya, Terminalia cattapa in soft cheese storage can suppress total aerobes and anaerobes Therefore their use in extension of the shelf-life of soft cheese is recommended.

  19. Production of functional killer protein in batch cultures upon a shift from aerobic to anaerobic conditions

    Directory of Open Access Journals (Sweden)

    Gildo Almeida da Silva

    2011-06-01

    Full Text Available The aim of this work was to study the production of functional protein in yeast culture. The cells of Saccharomyces cerevisiae Embrapa 1B (K+R+ killed a strain of Saccharomyces cerevisiae Embrapa 26B (K-R-in grape must and YEPD media. The lethal effect of toxin-containing supernatant and the effect of aeration upon functional killer production and the correlation between the products of anaerobic metabolism and the functional toxin formation were evaluated. The results showed that at low sugar concentration, the toxin of the killer strain of Sacch. cerevisiae was only produced under anaerobic conditions . The system of killer protein production showed to be regulated by Pasteur and Crabtree effects. As soon as the ethanol was formed, the functional killer toxin was produced. The synthesis of the active killer toxin seemed to be somewhat associated with the switch to fermentation process and with concomitant alcohol dehydrogenase (ADH activity.

  20. Post-treatment of anaerobically degraded azo dye Acid Red 18 using aerobic moving bed biofilm process: Enhanced removal of aromatic amines

    Energy Technology Data Exchange (ETDEWEB)

    Hosseini Koupaie, E., E-mail: ehssan.hosseini.k@gmail.com [Civil and Environmental Engineering Department, Amirkabir University of Technology (AUT), Hafez Ave., Tehran 15875-4413 (Iran, Islamic Republic of); Alavi Moghaddam, M.R., E-mail: alavim@yahoo.com [Civil and Environmental Engineering Department, Amirkabir University of Technology (AUT), Hafez Ave., Tehran 15875-4413 (Iran, Islamic Republic of); Hashemi, S.H., E-mail: h_hashemi@sbu.ac.ir [Environmental Science Research Institute, Shahid Beheshti University, Tehran (Iran, Islamic Republic of)

    2011-11-15

    Highlights: {yields} Biofilm process was applied as post-treatment of anaerobically degraded an azo dye. {yields} More than 65% of the dye total metabolites was completely mineralized. {yields} Based on HPLC analysis, more than 80% of 1-naphthylamine-4-sulfonate was removed. {yields} Inhibition of biofilm growth was increased with increasing the initial dye concentration. {yields} Considerable porous morphology was observed in the SEM photographs of the biofilm. - Abstract: The application of aerobic moving bed biofilm process as post-treatment of anaerobically degraded azo dye Acid Red 18 was investigated in this study. The main objective of this work was to enhance removal of anaerobically formed the dye aromatic metabolites. Three separate sequential treatment systems were operated with different initial dye concentrations of 100, 500 and 1000 mg/L. Each treatment system consisted of an anaerobic sequencing batch reactor (An-SBR) followed by an aerobic moving bed sequencing batch biofilm reactor (MB-SBBR). Up to 98% of the dye decolorization and more than 80% of the COD removal occurred anaerobically. The obtained results suggested no significant difference in COD removal as well as the dye decolorization efficiency using three An-SBRs receiving different initial dye concentrations. Monitoring the dye metabolites through HPLC suggested that more than 80% of anaerobically formed 1-naphthylamine-4-sulfonate was completely removed in the aerobic biofilm reactors. Based on COD analysis results, at least 65-72% of the dye total metabolites were mineralized during the applied treatment systems. According to the measured biofilm mass and also based on respiration-inhibition test results, increasing the initial dye concentration inhibited the growth and final mass of the attached-growth biofilm in MB-SBBRs.

  1. TBA biodegradation in surface-water sediments under aerobic and anaerobic conditions

    Science.gov (United States)

    Bradley, P.M.; Landmeyer, J.E.; Chapelle, F.H.

    2002-01-01

    The potential for [U-14C] TBA biodegradation was examined in laboratory microcosms under a range of terminal electron accepting conditions. TBA mineralization to CO2 was substantial in surface-water sediments under oxic, denitrifying, or Mn(IV)-reducing conditions and statistically significant but low under SO4-reducing conditions. Thus, anaerobic TBA biodegradation may be a significant natural attenuation mechanism for TBA in the environment, and stimulation of in situ TBA bioremediation by addition of suitable terminal electron acceptors may be feasible. No degradation of [U-14C] TBA was observed under methanogenic or Fe(III)-reducing conditions.

  2. The influence of hydrolysis induced biopolymers from recycled aerobic sludge on specific methanogenic activity and sludge filterability in an anaerobic membrane bioreactor.

    Science.gov (United States)

    Buntner, D; Spanjers, H; van Lier, J B

    2014-03-15

    The objective of the present study was to evaluate the impact of excess aerobic sludge on the specific methanogenic activity (SMA), in order to establish the maximum allowable aerobic sludge loading. In batch tests, different ratios of aerobic sludge to anaerobic inoculum were used, i.e. 0.03, 0.05, 0.10 and 0.15, showing that low ratios led to an increased SMA. However, the ratio 0.15 caused more than 20% SMA decrease. In addition to the SMA tests, the potential influence of biopolymers and extracellular substances, that are generated as a result of excess aerobic sludge hydrolysis, on membrane performance was determined by assessing the fouling potential of the liquid broth, taking into account parameters such as specific resistance to filtration (SRF) and supernatant filterability (SF). Addition of aerobic sludge to the anaerobic biomass resulted in a high membrane fouling potential. The increase in biopolymers could be ascribed to aerobic sludge hydrolysis. A clear positive correlation between the concentration of the colloidal fraction of biopolymer clusters (cBPC) and the SRF was observed and a negative correlation between the cBPC and the SF measured at the end of the above described SMA tests. The latter implies that sludge filtration resistance increases when more aerobic sludge is hydrolyzed, and thus more cBPC is released. During AnMBR operation, proteins significantly contributed to sludge filterability decrease expressed as SRF and SF, whereas the carbohydrate fraction of SMP was of less importance due to low concentrations. On the contrary, carbohydrates seemed to improve filterability and diminish SRF of the sludge. Albeit, cBPC increase caused an increase in mean TMP during the AnMBR operation, confirming that cBPC is positively correlated to membrane fouling.

  3. Fate of oestrogens during anaerobic blackwater treatment with micro-aerobic post-treatment.

    Science.gov (United States)

    de Mes, T Z D; Kujawa-Roeleveld, K; Zeeman, G; Lettinga, G

    2007-01-01

    The fate of oestrone (E1), 17beta-oestradiol (E2) and 17alpha-ethynyloestradiol (EE2) was investigated in a concentrated blackwater treatment system consisting of an UASB septic tank, with micro-aerobic post-treatment. In UASB septic tank effluent a (natural) total concentration of 4.02 microg/L E1 and 18.69 microg/L E2, comprising the sum of conjugated (>70% for E1 and >80% for E2) and unconjugated forms, was measured. During post-treatment the unconjugated oestrogens were removed to below 1 microg/L. A percentage of 77% of the measured unconjugated E1 and 82% of E2 was associated with particles >1.2 microm in the final effluent implying high sorption affinity of both compounds. When spiking the UASB septic tank effluent with E1, E2, EE2 and the sulphate conjugate of E2, removal in the micro-aerobic post-treatment was >99% for both E2 and EE2 and 83% for E1. The lower removal value for E1 was a result of (slow) deconjugation during the treatment, and in the final effluent still 40% of E1 and 99% of E2 was present in conjugated form. The latter was the result of incomplete deconjugation of the spiked E2(3S) in the post-treatment system.

  4. Analysis of the aerobic-anaerobic transition in elite cyclists during incremental exercise with the use of electromyography

    Science.gov (United States)

    Lucia, A.; Sanchez, O.; Carvajal, A.; Chicharro, J. L.

    1999-01-01

    OBJECTIVES: To investigate the validity and reliability of surface electromyography (EMG) as a new non-invasive determinant of the metabolic response to incremental exercise in elite cyclists. The relation between EMG activity and other more conventional methods for analysing the aerobic-anaerobic transition such as blood lactate measurements (lactate threshold (LT) and onset of blood lactate accumulation (OBLA)) and ventilatory parameters (ventilatory thresholds 1 and 2 (VT1 and VT2)) was studied. METHODS: Twenty eight elite road cyclists (age 24 (4) years; VO2MAX 69.9 (6.4) ml/kg/min; values mean (SD)) were selected as subjects. Each of them performed a ramp protocol (starting at 0 W, with increases of 5 W every 12 seconds) on a cycle ergometer (validity study). In addition, 15 of them performed the same test twice (reliability study). During the tests, data on gas exchange and blood lactate levels were collected to determine VT1, VT2, LT, and OBLA. The root mean squares of EMG signals (rms-EMG) were recorded from both the vastus lateralis and the rectus femoris at each intensity using surface electrodes. RESULTS: A two threshold response was detected in the rms-EMG recordings from both muscles in 90% of subjects, with two breakpoints, EMGT1 and EMGT2, at around 60-70% and 80-90% of VO2MAX respectively. The results of the reliability study showed no significant differences (p > 0.05) between mean values of EMGT1 and EMGT2 obtained in both tests. Furthermore, no significant differences (p > 0.05) existed between mean values of EMGT1, in the vastus lateralis and rectus femoris, and VT1 and LT (62.8 (14.5) and 69.0 (6.2) and 64.6 (6.4) and 68.7 (8.2)% of VO2MAX respectively), or between mean values of EMGT2, in the vastus lateralis and rectus femoris, and VT2 and OBLA (86.9 (9.0) and 88.0 (6.2) and 84.6 (6.5) and 87.7 (6.4)% of VO2MAX respectively). CONCLUSION: rms-EMG may be a useful complementary non-invasive method for analysing the aerobic- anaerobic transition

  5. Biodegradation of Reactive blue 13 in a two-stage anaerobic/aerobic fluidized beds system with a Pseudomonas sp. isolate.

    Science.gov (United States)

    Lin, Jun; Zhang, Xingwang; Li, Zhongjian; Lei, Lecheng

    2010-01-01

    Pseudomonas sp. strain L1 capable of degrading the azo textile dye Reactive blue 13, was isolated from activated sludge in a sequencing batch reactor. A continuous two-stage anaerobic/aerobic biological fluidized bed system was used to decolorize and mineralize Reactive blue 13. The key factors affecting decolorization were investigated and the efficiency of degradation was also optimized. An overall color removal of 83.2% and COD removal of 90.7% was achieved at pH 7, a residence time of 70 h and a glucose concentration of 2 g/L, HRT=70 h and C(glucose)=2000 mg/L. Oxygen was contributing to blocking the azo bond cleavage. Consequently, decolorization occurred in the anaerobic reactor while partial mineralization was achieved in the aerobic reactor. A possible degradation pathway based on the analysis of intermediates and involving azoreduction, desulfonation, deamination and further oxidation reactions is presented.

  6. The investigation of effect of organic carbon sources addition in anaerobic-aerobic (low dissolved oxygen) sequencing batch reactor for nutrients removal from wastewaters.

    Science.gov (United States)

    Zheng, Xiong; Tong, Juan; Li, Hongjing; Chen, Yinguang

    2009-05-01

    The effect of addition of organic carbon sources (acetic acid and waste activated sludge alkaline fermentation liquid) on anaerobic-aerobic (low dissolved oxygen, 0.15-0.45 mg/L) biological municipal wastewater treatment was investigated. The results showed that carbon source addition affected not only the transformations of polyhydroxyalkanoates (PHA), glycogen, nitrogen and phosphorus, but the net removal of nitrogen and phosphorus. The removal efficiencies of TN and TP were, respectively, 61% and 61% without organic carbon source addition, 81% and 95% with acetic acid addition, and 83% and 97% with waste activated sludge alkaline fermentation liquid addition. It seems that the alkaline fermentation liquid of waste biosolids generated in biological wastewater treatment plant can be used to replace acetic acid as an additional carbon source to improve the anaerobic-aerobic (low dissolved oxygen) municipal wastewater nutrients removal although its use was observed to cause a slight increase of effluent BOD and COD concentrations.

  7. Neisseria gonorrhoeae survive intraleukocytic oxygen-independent antimicrobial capacities of anaerobic and aerobic granulocytes in the presence of pyocin lethal for extracellular gonococci.

    Science.gov (United States)

    Casey, S G; Shafer, W M; Spitznagel, J K

    1986-05-01

    The resistance of a piliated, transparent variant of Neisseria gonorrhoeae FA19 to intraleukocytic killing by human polymorphonuclear neutrophils (PMN) was examined. In both aerobic and anaerobic PMN monolayers, approximately 2% of the intracellular gonococci survived for as long as 165 min. Anaerobic PMN were as effective as aerobic PMN in the intracellular killing of gonococci. Hence, O2-independent antimicrobial systems of PMN performed a significant role in the intraleukocytic killing of gonococci were intracellular was supported by the elimination of extracellular bacteria by the addition of pyocin 103 and confirmed by the fluorescent antibody staining of intact gonococci after the PMN were permeabilized to antibody with a Formalin-acetone treatment of PMN monolayers. Our data indicate that while the majority of ingested gonococci are killed by O2-independent antimicrobial systems, a small number (about 2%), survive even when care is taken to eliminate extracellular bacteria.

  8. Assessment and Evaluation of an Integrated Hybrid Anaerobic-Aerobic Sewage Treatment System for the Removal of Enteric Viruses.

    Science.gov (United States)

    El-Senousy, Waled Morsy; Abou-Elela, Sohair Imam

    2017-02-14

    The capability of a cost-effective and a small size decentralized pilot wastewater treatment plant (WWTP) to remove enteric viruses such as rotavirus, norovirus genogroup I (GGI), norovirus genogroup II (GGII), Hepatitis E virus (HEV), and adenovirus was studied. This pilot plant is an integrated hybrid anaerobic/aerobic setup which consisted of anaerobic sludge blanket (UASB), biological aerated filter (BAF), and inclined plate settler (IPS). Both the UASB and BAF are packed with a non-woven polyester fabric (NWPF). Results indicated that the overall log10 reductions of enteric viruses' genome copies through the whole system were 3.1 ± 1, 3.3 ± 0.5, and 2.6 ± 0.9 log10 for rotavirus, norovirus GGI, and adenovirus, respectively. Reduction efficiency for both norovirus GGII and HEV after the different treatment steps could not be calculated because there were no significant numbers of positive samples for both viruses. The overall reduction of rotavirus infectious units through the whole system was 2.2 ± 0.8 log10 reduction which is very close to the overall log10 reduction of adenovirus infectious units through the whole system which was 2.1 ± 0.8 log10 reduction. There was no considerable difference in the removal efficiency for different rotavirus G and P types. Adenovirus 41 was the only type detected in the all positive samples. Although the pilot WWTP investigated is cost effective, has a small footprint, does not need a long distance network pipes, and easy to operate, its efficiency to remove enteric viruses is comparable with the conventional centralized WWTPs.

  9. Anaerobic and aerobic peak power output and the force-velocity relationship in endurance-trained athletes: effects of aging.

    Science.gov (United States)

    Chamari, K; Ahmaidi, S; Fabre, C; Massé-Biron, J; Préfaut, C

    1995-01-01

    The aim of this investigation was to test the hypothesis that the anaerobic peak power output (Pan, peak) declines more than the peak aerobic power (Paer, peak) with increasing age. In addition, the force-velocity (F-v) relationship was studied to determine which of these two factors is primarily responsible for the expected alterations in anaerobic power. The Pan, peak, the maximal F when v is equal to zero (F0) and the maximal v when F is equal to zero (v0) were assessed by F-v test i.e. a brief intense intermittent exercise test using incremental braking forces. The Paer, peak was measured by a maximal increment exercise test. A group of 12 young athletes (YA) and 12 master athletes (MA) mean age 24.8 (SEM 1.3) and 65.1 (SEM 1.2) years, respectively, participated in this study. The YA and MA had similar body masses, heights and endurance training schedules. The results showed that Pan, peak was 42.7% lower in the older subjects, corresponding to mean values of 1089 (SEM 40) compared to 624 (SEM 33) W (t = 8.9, P Paer, peak was 35% lower with mean values of 323 (SEM 12) W for YA compared to 210 (SEM 6) W for MA (t = 8.3, P < 0.001). The mean maximal oxygen uptake was 34.7% lower with 4240 (SEM 160) ml.min-1 for YA compared to 2770 (SEM 120) ml.min-1 for MA (t = 7.2, P < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

  10. Physiological roles of ArcA, Crp, and EtrA and their interactive control on aerobic and anaerobic respiration in Shewanella oneidensis

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Haichun [Zhejiang University; Wang, Xiaohu [Baylor College of Medicine, Huston; Chen, Jingrong [University of Oklahoma, Norman; Liang, Yili [University of Oklahoma, Norman; Chen, Haijiang [Zhejiang University; Palzkill, Timothy [Baylor College of Medicine, Huston; Zhou, Jizhong [University of Oklahoma

    2010-01-01

    In the genome of Shewanella oneidensis, genes encoding the global regulators ArcA, Crp, and EtrA have been identified. All these proteins deviate from their counterparts in E. coli significantly in terms of functionality and regulon. It is worth investigating the involvement and relationship of these global regulators in aerobic and anaerobic respiration in S. oneidensis. In this study, the impact of the transcriptional factors ArcA, Crp, and EtrA on aerobic and anaerobic respiration in S. oneidensis were assessed. While all these proteins appeared to be functional in vivo, the importance of individual proteins in these two major biological processes differed. The ArcA transcriptional factor was critical in aerobic respiration while the Crp protein was indispensible in anaerobic respiration. Using a newly developed reporter system, it was found that expression of arcA and etrA was not influenced by growth conditions but transcription of crp was induced by removal of oxygen. An analysis of the impact of each protein on transcription of the others revealed that Crp expression was independent of the other factors whereas ArcA repressed both etrA and its own transcription while EtrA also repressed arcA transcription. Transcriptional levels of arcA in the wild type, crp, and etrA strains under either aerobic or anaerobic conditions were further validated by quantitative immunoblotting with a polyclonal antibody against ArcA. This extensive survey demonstrated that all these three global regulators are functional in S. oneidensis. In addition, the reporter system constructed in this study will facilitate in vivo transcriptional analysis of targeted promoters.

  11. ANAEROBIC-AEROBIC TREATMENT OF TEXTILE WASTEWATER IN A SEQUENCING BATCH REACTOR

    Directory of Open Access Journals (Sweden)

    IBTISSAM KANBOUCHI

    2014-04-01

    Full Text Available In this work, the treatment of synthetic textile wastewater using sequential batch reactor (SBR was studied. This in order to predict the effectiveness of biological treatment on wastewater containing dyes while minimizing the aeration cost. Laboratory tests were performed on synthetic wastewater containing filtered urban wastewater (source of bacteria and dyes solutions. This promotes the biomass development in the mixture, capable of degrading organic matter properly. The results indicate that the increasing of anaerobic phase (16 hours allows removal of 77 % and 80 % of COD and colour, respectively. The sludge age did not affect markedly dyes biodegradability. However, the biodegradability is strongly influenced by the dyes concentration. Indeed, for the lowest dyes contents, improved biodegradability was observed, while it decreases when the dyes concentration increases.

  12. Biological wastewater treatment by a bioreactor with repeated coupling of aerobes and anaerobes aiming at on-site reduction of excess sludge.

    Science.gov (United States)

    Yu, Anfeng; Feng, Quan; Liu, Zehua; Zhou, Yunan; Xing, Xin-Hui

    2006-01-01

    Activated sludge has been widely used in wastewater treatment throughout the world. However, the biggest disadvantage of this method is the by-production of excess sludge in a large amount, resulting in difficulties in operation and high costs for wastewater treatment. Technological innovations for wastewater treatment capable of reducing excess sludge have thus become research topics of interest in recent years. In our present research, we developed a new biological wastewater treatment process by repeated coupling of aerobes and anaerobes (rCAA) to reduce the excess sludge during the treatment of wastewater. During 460-day continuous running, COD (300-700 mg/L) and TOC (100-350 mg/L) were effectively removed, of which the removal rate was above 80 and 90%, respectively. SS in the effluent was 13 mg/L on average in the rCAA bioreactor without a settling tank. The on-site reduction of the excess sludge in the rCAA might be contributed by several mechanisms. The degradation of the grown aerobes after moving into the anaerobic regions was considered to be one of the most important factors. Besides, the repeatedly coupling of aerobes and anaerobes could also result in a complex microbial community with more metazoans and decoupling of the microbial anabolism and catabolism.

  13. Relationships between chemical oxygen demand (COD) components and toxicity in a sequential anaerobic baffled reactor/aerobic completely stirred reactor system treating Kemicetine.

    Science.gov (United States)

    Sponza, Delia Teresa; Demirden, Pinar

    2010-04-15

    In this study the interactions between toxicity removals and Kemicetine, COD removals, intermediate products of Kemicetine and COD components (CODs originating from slowly degradable organics, readily degradable organics, inert microbial products and from the inert compounds) were investigated in a sequential anaerobic baffled reactor (ABR)/aerobic completely stirred tank reactor (CSTR) system with a real pharmaceutical wastewater. The total COD and Kemicetine removal efficiencies were 98% and 100%, respectively, in the sequential ABR/CSTR systems. 2-Amino-1 (p-nitrophenil)-1,3 propanediol, l-p-amino phenyl, p-amino phenol and phenol were detected in the ABR as the main readily degradable inter-metabolites. In the anaerobic ABR reactor, the Kemicetin was converted to corresponding inter-metabolites and a substantial part of the COD was removed. In the aerobic CSTR reactor the inter-metabolites produced in the anaerobic reactor were completely removed and the COD remaining from the anerobic reactor was biodegraded. It was found that the COD originating from the readily degradable organics did not limit the anaerobic degradation process, while the CODs originating from the slowly degradable organics and from the inert microbial products significantly decreased the anaerobic ABR reactor performance. The acute toxicity test results indicated that the toxicity decreased from the influent to the effluent of the aerobic CSTR reactor. The ANOVA test statistics showed that there was a strong linear correlation between acute toxicity, CODs originating from the slowly degradable organics and inert microbial products. A weak correlation between acute toxicity and CODs originating from the inert compounds was detected.

  14. Decolourization of anaerobically digested and polyaluminium chloride treated distillery spentwash in a fungal stirred tank aerobic reactor.

    Science.gov (United States)

    Singh, S S; Dikshit, A K

    2011-11-01

    Decolourization of anaerobically digested and polyaluminium chloride treated distillery spentwash was studied in a fungal stirred tank aerobic reactor without dilution of wastewater. Aspergillus niger isolate IITB-V8 was used as the fungal inoculum. The main objectives of the study were to optimize the stirrer speed for achieving maximum decolourization and to determine the kinetic parameters. A mathematical model was developed to describe the batch culture kinetics. Volumetric oxygen transfer coefficient (k (L) a) was obtained using dynamic method. The maximum specific growth rate and growth yield of fungus were determined using Logistic equation and using Luedeking-Piret equation. 150 rpm was found to be optimum stirrer speed for overall decolourization of 87%. At the optimum stirrer speed, volumetric oxygen transfer coefficient (k (L) a) was 0.4957 min(-1) and the maximum specific growth rate of fungus was 0.224 h(-1). The values of yield coefficient (Y ( x/s)) and maintenance coefficient (m (s)) were found to be 0.48 g cells (g substrate)(-1) and 0.015 g substrate (g cells)(-1) h(-1).

  15. Influence of aeration modes on leachate characteristic of landfills that adopt the aerobic-anaerobic landfill method.

    Science.gov (United States)

    Wu, Chuanfu; Shimaoka, Takayuki; Nakayama, Hirofumi; Komiya, Teppei; Chai, Xiaoli; Hao, Yongxia

    2014-01-01

    As far as the optimal design, operation, and field application of the Aerobic-Anaerobic Landfill Method (AALM) are concerned, it is very important to understand how aeration modes (different combinations of aeration depth and air injection rate) affect the biodegradation of organic carbon and the transformation of nitrogen in landfill solid waste. Pilot-scale lysimeter experiments were carried out under different aeration modes to obtain detailed information regarding the influence of aeration modes on leachate characteristics. Results from these lysimeter experiments revealed that aeration at the bottom layer was the most effective for decomposition of organic carbon when compared with aeration at the surface or middle layers. Moreover, the air injection rate led to different nitrogen transformation patterns, unlike the lesser influence it has on organic carbon decomposition. Effective simultaneous nitrification and denitrification were observed for the aeration mode with a higher air injection rate (=1.0 L/min). On the other hand, the phenomenon of sequenced nitrification and denitrification could be observed when a low air injection rate (=0.5L/min.) was employed. Finally, it is concluded that, for AALM, air injection with a higher air injection rate at the deepest layer near the leachate collection pipe tends to accelerate the stabilization of landfill waste as defined in terms of the enhancement of denitrification as well as organic carbon decomposition.

  16. The effect of extensive interval training at altitude on the physiological, aerobic, anaerobic and various blood parameters of athletes

    Directory of Open Access Journals (Sweden)

    Kaya Ismail

    2016-01-01

    Full Text Available In this research, it was aimed to compare the physiological performances of athletes at sea level, at high altitude and 8 days after returning back to sea level on the basis of certain blood parameters, pulse and blood pressure. 12 male athletes between the ages of 19 and 23 voluntarily participated in the research. The subjects were exposed to endurance training at high altitude and at sea level between 09.00 and 11.00 in the morning. The subjects’ erythrocyte (RBC, leucocyte (WBC, haemoglobin (Hb, haematocrit (HCT, systolic blood pressure at rest (SBPR and diastolic blood pressure at rest (DBPR, heart rate at rest (HRR, aerobic (20m shuttle run test and anaerobic capacity (vertical jump levels were tested at sea level, on the 15th day at high altitude (3120m and 8 days after returning back to sea level. Statistical analysis comprised of t-test and the significance level of the results was accepted at (P<0.05. As a result of the research the following were determined: It can be said that high altitude trainings for fifteen days included in the annual training program of athletes can improve their performance.

  17. Antibiotic resistance of Escherichia coli in leachates from municipal solid waste landfills: comparison between semi-aerobic and anaerobic operations.

    Science.gov (United States)

    Threedeach, Simanata; Chiemchaisri, Wilai; Watanabe, Toru; Chiemchaisri, Chart; Honda, Ryo; Yamamoto, Kazuo

    2012-06-01

    A bacterial susceptibility test to 31 antibiotics was conducted on the 80 isolated Escherichia coli from the leachates of the landfills in two different operations: semi-aerobic (SL) and anaerobic (AL) landfills. The average population of E. coli from SL and AL leachates were 2.2 × 10(4) and 9.4 × 10(3)CFU/100ml, respectively. Between 80.8% and 87.5% of the E. coli isolated from both leachates as resistant to the tested antibiotics and high resistances were found to doxycycline (53.8-68.8%), cephalothin (57.5-61.3%), tetracycline (51.3-67.5%) and minocycline (37.5-46.3%). The percentages of isolates resistant to most antibiotics were higher in the AL leachate. The exception was nitrofurantoin to which the isolates from the SL were highly resistant. E. coli from both sources showed similarly high susceptibilities (90-100%) to aminoglycosides, quinolones, chloramphenicol, sulfonamide and some new beta-lactams. The difference in available oxygen in landfills could affect E. coli susceptibility to antibiotics in leachates.

  18. Fast dynamic response of the fermentative metabolism of Escherichia coli to aerobic and anaerobic glucose pulses.

    Science.gov (United States)

    Lara, Alvaro R; Taymaz-Nikerel, Hilal; Mashego, Mlawule R; van Gulik, Walter M; Heijnen, Joseph J; Ramírez, Octavio T; van Winden, Wouter A

    2009-12-15

    The response of Escherichia coli cells to transient exposure (step increase) in substrate concentration and anaerobiosis leading to mixed-acid fermentation metabolism was studied in a two-compartment bioreactor system consisting of a stirred tank reactor (STR) connected to a mini-plug-flow reactor (PFR: BioScope, 3.5 mL volume). Such a system can mimic the situation often encountered in large-scale, fed-batch bioreactors. The STR represented the zones of a large-scale bioreactor that are far from the point of substrate addition and that can be considered as glucose limited, whereas the PFR simulated the region close to the point of substrate addition, where glucose concentration is much higher than in the rest of the bioreactor. In addition, oxygen-poor and glucose-rich regions can occur in large-scale bioreactors. The response of E. coli to these large-scale conditions was simulated by continuously pumping E. coli cells from a well stirred, glucose limited, aerated chemostat (D = 0.1 h(-1)) into the mini-PFR. A glucose pulse was added at the entrance of the PFR. In the PFR, a total of 11 samples were taken in a time frame of 92 s. In one case aerobicity in the PFR was maintained in order to evaluate the effects of glucose overflow independently of oxygen limitation. Accumulation of acetate and formate was detected after E. coli cells had been exposed for only 2 s to the glucose-rich (aerobic) region in the PFR. In the other case, the glucose pulse was also combined with anaerobiosis in the PFR. Glucose overflow combined with anaerobiosis caused the accumulation of formate, acetate, lactate, ethanol, and succinate, which were also detected as soon as 2 s after of exposure of E. coli cells to the glucose and O(2) gradients. This approach (STR-mini-PFR) is useful for a better understanding of the fast dynamic phenomena occurring in large-scale bioreactors and for the design of modified strains with an improved behavior under large-scale conditions.

  19. Coenzyme B12 can be produced by engineered Escherichia coli under both anaerobic and aerobic conditions.

    Science.gov (United States)

    Ko, Yeounjoo; Ashok, Somasundar; Ainala, Satish Kumar; Sankaranarayanan, Mugesh; Chun, Ah Yeong; Jung, Gyoo Yeol; Park, Sunghoon

    2014-12-01

    Coenzyme B12 (Vitamin B12 ) is one of the most complex biomolecules and an essential cofactor required for the catalytic activity of many enzymes. Pseudomonas denitrificans synthesizes coenzyme B12 in an oxygen-dependent manner using a pathway encoded by more than 25 genes that are located in six different operons. Escherichia coli, a robust and suitable host for metabolic engineering was used to produce coenzyme B12 . These genes were cloned into three compatible plasmids and expressed heterologously in E. coli BL21 (DE3). Real-time PCR, SDS-PAGE analysis and bioassay showed that the recombinant E. coli expressed the coenzyme B12 synthetic genes and successfully produced coenzyme B12 . However, according to the quantitative determination by inductively coupled plasma-mass spectrometry, the amount of coenzyme B12 produced by the recombinant E. coli (0.21 ± 0.02 μg/g cdw) was approximately 13-fold lower than that by P. denitrificans (2.75 ± 0.22 μg/g cdw). Optimization of the culture conditions to improve the production of coenzyme B12 by the recombinant E. coli was successful, and the highest titer (0.65 ± 0.03 μg/g cdw) of coenzyme B12 was obtained. Interestingly, although the synthesis of coenzyme B12 in P. denitrificans is strictly oxygen-dependent, the recombinant E. coli could produce coenzyme B12 under anaerobic conditions.

  20. Evolution of Electron Transport Chains During the Anaerobic to Aerobic Transition on Early Earth

    Science.gov (United States)

    Sepúlveda, R.; Ortiz, R.; Holmes, D. S.

    2015-12-01

    Sepulveda, R., Ortiz R. and Holmes DS. Center for Bioinformatics and Genome Biology, Fundacion Ciencia y Vida, and Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile.According to several models, life emerged on earth in an anoxic environment where oxygen was not available as a terminal electron acceptor for energy generating reactions. After the Great Oxidation Event (GOE) about 2.4 billion years ago, or perhaps even before the GOE, oxygen became the most widespread and efficient terminal electron acceptor and was accompanied by the evolution of a number of redox proteins that could deliver electrons to reduce oxygen to water. Where did these proteins come from? One hypothesis is that they evolved by the neofunctionalization of previously existing redox proteins that had been used in anaerobic conditions as terminal electron donors to reduce compounds such as perchlorate, nitric oxide or iron. We have used a number of bioinformatic tools to explore a large number of genomes looking for discernable signals of such redeployment of function. A Perl pipeline was designed to detect sequence similarity, conserved gene context, remote homology detection, identification of domains and functional evolution of electron carrier proteins from extreme acidophiles, including the small blue copper protein rusticyanin (involved in FeII oxidation), cytochrome oxidase subunit II and quinol-dependent nitric oxide reductase (qNOR). The protein folds and copper binding sites of rusticyanin are conserved in cytochrome oxidase aa3 subunit II, a protein complex that is responsible for the final passage of electrons to reduce oxygen. Therefore, we hypothesize that rusticyanin, cytochrome oxidase II and qNOR are evolutionarily related. Acknowledgments: Fondecyt 1130683.

  1. Investigation of Anaerobic Fluidized Bed Reactor Aerobic Mov-ing Bed Bio Reactor (AFBR/MMBR System for Treatment of Currant Wastewater

    Directory of Open Access Journals (Sweden)

    Jalil Jafari

    2013-08-01

    Full Text Available Background: Anaerobic treatment methods are more suitable for the treatment of concentrated wastewater streams, offer lower operating costs, the production of usable biogas product. The aim of this study was to investigate the performance of an Anaerobic Fluidized Bed Reactor (AFBR-Aerobic Moving Bed Bio Reactor (MBBR in series arrangement to treat Currant wastewater.Methods: The bed materials of AFBR were cylindrical particles made of PVC with a diameter of 2-2.3 mm, particle density of 1250 kg/m3.The volume of all bed materials was 1.7 liter which expanded to 2.46 liters in fluidized situation. In MBBR, support media was composed of 1.5 liters Bee-Cell 2000 having porosity of 87% and specific surface area of 650m2/m3.Results: When system operated at 35 ºC, chemical oxygen demand (COD removal efficiencies were achieved to 98% and 81.6% for organic loading rates (OLR of 9.4 and 24.2 g COD/l.d, and hydraulic retention times (HRT of 48 and 18 h, in average COD concentration feeding of 18.4 g/l, respectively.Conclusion: The contribution of AFBR in total COD removal efficiency at an organic loading rate (OLR of 9.4 g COD/l.d was 95%, and gradually decreased to 76.5% in OLR of 24.2 g COD/l.d. Also with increasing in organic loading rate the contribution of aerobic reactor in removing COD gradually decreased. In this system, the anaerobic reactor played the most important role in the removal of COD, and the aerobic MBBR was actually needed to polish the anaerobic treated wastewate

  2. Anaerobic lactic acid degradation during ensilage of whole crop maize inoculated with lactobacillus buchneri inhibits yeast growth and improves aerobic stability

    Science.gov (United States)

    Driehuis; Elferink; Spoelstra

    1999-10-01

    Aerobic deterioration of silages is initiated by (facultative) aerobic micro-organisms, usually yeasts, that oxidize the preserving organic acids. In this study, a Lactobacillus buchneri strain isolated from maize silage was evaluated for its potential as a bacterial inoculant that enhances aerobic stability of silages. In four experiments, chopped whole crop maize (30-43% dry matter (DM)) was inoculated with Lact. buchneri and ensiled in laboratory silos. Uninoculated silages served as controls. Analysis of silages treated with Lact. buchneri at levels of 103-106 cfu g-1 after about 3 months of anaerobic storage showedthat acetic acid and 1-propanol contents increased with inoculum levels above 104 cfu g-1,whereas lactic acid decreased. Propionic acid, silage pH and DM loss increased withinoculum levels above 105 cfu g-1. Time course experiments with maize inoculated with Lact. buchneri at 4 x 104-2 x 105 cfu g-1 showed that up to 7-14 d after ensiling, Lact. buchneri had no effect on silage characteristics. Thereafter, the lactic acid content of the inoculated silages declined and, simultaneously, acetic acid and, to a lesser extent, propionic acid and 1-propanol, accumulated. Inoculation reduced survival of yeasts during the anaerobic storage phase and inhibited yeast growth when the silage was exposed to O2, resulting in a substantial improvement in aerobic stability. The results indicate that the use of Lact. buchneri as a silage inoculant can enhance aerobic stability by inhibition of yeasts. The ability of the organism to ferment lactic acid to acetic acid appears to be an important underlying principle of this effect.

  3. Detection of Bacterial and Yeast Species with the Bactec 9120 Automated System with Routine Use of Aerobic, Anaerobic, and Fungal Media▿

    Science.gov (United States)

    Chiarini, Alfredo; Palmeri, Angelo; Amato, Teresa; Immordino, Rita; Distefano, Salvatore; Giammanco, Anna

    2008-01-01

    During the period 2006 and 2007, all blood cultures required by four units at high infective risk and most of those required by other units of the University Hospital of Palermo, Palermo, Italy were performed using a Bactec 9120 automated blood culture system with a complete set of Plus Aerobic/F, Plus Anaerobic/F, and Mycosis IC/F bottles. The aim of the study was to enable the authors to gain firsthand experience of the culture potentialities of the three different media, to obtain information regarding the overall and specific recovery of bacteria and yeasts from blood cultures in the hospital, and to reach a decision as to whether and when to utilize anaerobic and fungal bottles. Although very few bloodstream infections (1.8%) were associated with obligate anaerobes, the traditional routine use of anaerobic bottles was confirmed because of their usefulness, not only in the detection of anaerobes, but also in that of gram-positive cocci and fermentative gram-negative bacilli. In this study, Mycosis IC/F bottles detected 77.4% of all the yeast isolates, 87.0% of yeasts belonging to the species Candida albicans, and 45.7% of nonfermentative gram-negative bacilli resistant to chloramphenicol and tobramycin. In order to improve the diagnosis of fungemia in high-risk patients, the additional routine use of fungal bottles was suggested when, as occurred in the intensive-care unit and in the hematology unit of the University Hospital of Palermo, high percentages of bloodstream infections are associated with yeasts, and/or antibiotic-resistant bacteria and/or multiple bacterial isolates capable of inhibiting yeast growth in aerobic bottles. PMID:18923011

  4. Critical velocity, anaerobic distance capacity, maximal instantaneous velocity and aerobic inertia in sprint and endurance young swimmers.

    Science.gov (United States)

    Zacca, Rodrigo; Wenzel, Bruno München; Piccin, Jeferson Steffanello; Marcilio, Nilson Romeu; Lopes, André Luiz; de Souza Castro, Flávio Antônio

    2010-09-01

    Critical velocity (CV), anaerobic distance capacity (ADC), maximal instantaneous velocity (V (max)), and aerobic inertia (tau) were calculated from two (CV(2par) and ADC(2par)), three (CV(3par), ADC(3par), and V (max 3par)), and four-parameter model data (CV(4par), ADC(4par), V (max 4par), and tau), which were obtained from six different times and distances (50, 100, 200, 400, 800, and 1,500 m) swum in front crawl stroke under maximal intensity. Fourteen swimmers (14-15-year-old; sprint and endurance groups, each group n = 7) volunteered in this study. CV values were not influenced by the groups. The model effects showed that CV(2par) was higher than CV(3par) and CV(4par) regardless of the group used. In addition, CV(3par) and CV(4par) were similar. ADC seems to be better estimated using both three- and four-parameter models. V (max) was higher in the sprint group regardless of the model used. The models effects showed that the V (max 4par) was higher than the V (max 3par) regardless of the group. Sprint and endurance groups showed similar tau values. The analysis of the models (F test, coefficient of determination R (2), and adjusted coefficient of determination R (adjusted) (2) ) showed that the three-parameter model was more appropriate among the applied models. Although the four-parameter model showed better correlation for the endurance group, the inclusion of tau (fourth parameter) did not significantly improve the quality of adjustment. However, it is important to emphasize the availability of another parameter for the study of bioenergetics in swimming and other sports.

  5. Potential of Rhodobacter capsulatus Grown in Anaerobic-Light or Aerobic-Dark Conditions as Bioremediation Agent for Biological Wastewater Treatments

    Directory of Open Access Journals (Sweden)

    Stefania Costa

    2017-02-01

    Full Text Available The use of microorganisms to clean up wastewater provides a cheaper alternative to the conventional treatment plant. The efficiency of this method can be improved by the choice of microorganism with the potential of removing contaminants. One such group is photosynthetic bacteria. Rhodobacter capsulatus is a purple non-sulfur bacterium (PNSB found to be capable of different metabolic activities depending on the environmental conditions. Cell growth in different media and conditions was tested, obtaining a concentration of about 108 CFU/mL under aerobic-dark and 109 CFU/mL under anaerobic-light conditions. The biomass was then used as a bioremediation agent for denitrification and nitrification of municipal wastewater to evaluate the potential to be employed as an additive in biological wastewater treatment. Inoculating a sample of mixed liquor withdrawn from the municipal wastewater treatment plant with R. capsulatus grown in aerobic-dark and anaerobic-light conditions caused a significant decrease of N-NO3 (>95%, N-NH3 (70% and SCOD (soluble chemical oxygen demand (>69%, independent of the growth conditions. A preliminary evaluation of costs indicated that R. capsulatus grown in aerobic-dark conditions could be more convenient for industrial application.

  6. Consecutive anaerobic-aerobic treatment of the organic fraction of municipal solid waste and lignocellulosic materials in laboratory-scale landfill-bioreactors.

    Science.gov (United States)

    Pellera, Frantseska-Maria; Pasparakis, Emmanouil; Gidarakos, Evangelos

    2016-10-01

    The scope of this study is to evaluate the use of laboratory-scale landfill-bioreactors, operated consecutively under anaerobic and aerobic conditions, for the combined treatment of the organic fraction of municipal solid waste (OFMSW) with two different co-substrates of lignocellulosic nature, namely green waste (GW) and dried olive pomace (DOP). According to the results such a system would represent a promising option for eventual larger scale applications. Similar variation patterns among bioreactors indicate a relatively defined sequence of processes. Initially operating the systems under anaerobic conditions would allow energetic exploitation of the substrates, while the implementation of a leachate treatment system ultimately aiming at nutrient recovery, especially during the anaerobic phase, could be a profitable option for the whole system, due to the high organic load that characterizes this effluent. In order to improve the overall effectiveness of such a system, measures towards enhancing methane contents of produced biogas, such as substrate pretreatment, should be investigated. Moreover, the subsequent aerobic phase should have the goal of stabilizing the residual materials and finally obtain an end material eventually suitable for other purposes.

  7. Modeling microbial ethanol production by E. coli under aerobic/anaerobic conditions: applicability to real postmortem cases and to postmortem blood derived microbial cultures.

    Science.gov (United States)

    Boumba, Vassiliki A; Kourkoumelis, Nikolaos; Gousia, Panagiota; Economou, Vangelis; Papadopoulou, Chrissanthy; Vougiouklakis, Theodore

    2013-10-10

    The mathematical modeling of the microbial ethanol production under strict anaerobic experimental conditions for some bacterial species has been proposed by our research group as the first approximation to the quantification of the microbial ethanol production in cases where other alcohols were produced simultaneously with ethanol. The present study aims to: (i) study the microbial ethanol production by Escherichia coli under controlled aerobic/anaerobic conditions; (ii) model the correlation between the microbial produced ethanol and the other higher alcohols; and (iii) test their applicability in: (a) real postmortem cases that had positive BACs (>0.10 g/L) and co-detection of higher alcohols and 1-butanol during the original ethanol analysis and (b) postmortem blood derived microbial cultures under aerobic/anaerobic controlled experimental conditions. The statistical evaluation of the results revealed that the formulated models were presumably correlated to 1-propanol and 1-butanol which were recognized as the most significant descriptors of the modeling process. The significance of 1-propanol and 1-butanol as descriptors was so powerful that they could be used as the only independent variables to create a simple and satisfactory model. The current models showed a potential for application to estimate microbial ethanol - within an acceptable standard error - in various tested cases where ethanol and other alcohols have been produced from different microbes.

  8. The determination of the real nano-scale sizes of bacteria in chernozem during microbial succession by means of hatching of a soil in aerobic and anaerobic conditions

    Science.gov (United States)

    Gorbacheva, M.

    2012-04-01

    M.A. Gorbacheva,L.M. Polyanskaya The Faculty of Soil Science, Moscow State University, Leninskie Gory, GSP-1, Moscow,119991,Russia In recent years there's been particular attention paid to the smallest life's forms- bacteria which size can be measured in nanometer. These are the forms of bacteria with diameter of 5-200 nm. Theoretical calculations based on the content of the minimum number of DNA, enzyme, lipids in and ribosome in cells indicates impossibility of existence of a living cells within diameter less than 300 nm. It is theoretically possible for a living cell to exist within possible diameter of approximately 140 nm. Using a fluorescence microscope there's been indicated in a number of samples from lakes, rivers, soil, snow and rain water that 200 nm is the smallest diameter of a living cell. Supposingly, such a small size of bacteria in soil is determined by natural conditions which limit their development by nutritious substances and stress-factors. Rejuvenescence of nanobacteria under unfavourable natural conditions and stress-factors is studied in laboratory environment. The object of the current study has become the samples of typical arable chernozem of the Central Chernozem State Biosphere Reserve in Kursk. The detailed morphological description of the soil profile and its basic analytical characteristics are widely represented in scientific publications. The soil is characterized by a high carbon content which makes up 3,96% ,3,8% , and 2,9% for the upper layers of the A horizon, and 0,79% for the layer of the B horizon. A microbial succession was studied under aerobic and anaerobic conditions by means of experiments with microcosms in upper A horizons and B horizon of a chernozem. The final aim is to identify the cells size of bacteria in aerobic and anaerobic soil conditions in chernozem during the microbial succession, by dampening and application of chitin by means of «cascade filtration» method. The study of the microcosms is important for

  9. Free radicals: how do we stand them? Anaerobic and aerobic free radical (chain) reactions involved in the use of fluorogenic probes and in biological systems.

    Science.gov (United States)

    Liochev, Stefan I

    2014-01-01

    Biologically significant conclusions have been based on the use of fluorogenic and luminogenic probes for the detection of reactive species. The basic mechanisms of the processes involved have not been satisfactorily elucidated. In the present work, the mechanism of the enzyme and photosensitized oxidation of NAD(P)H by resorufin is analyzed and appears to involve both aerobic and anaerobic free radical chain reactions. There are two major fallouts of this analysis. Many of the conclusions about the participation of radicals based on the use of probes such as resorufin and Amplex red need reevaluation. It is also concluded that anaerobic free radical reactions may be biologically significant, and the possible existence of enzymatic systems to eliminate certain free radicals is discussed.

  10. Transcriptional regulation of the outer membrane porin gene ompW reveals its physiological role during the transition from the aerobic to the anaerobic lifestyle of Escherichia coli

    Directory of Open Access Journals (Sweden)

    Minfeng eXiao

    2016-05-01

    Full Text Available Understanding bacterial physiology relies on elucidating the regulatory mechanisms and cellular functions of those differentially expressed genes in response to environmental changes. A widespread Gram-negative bacterial outer membrane protein OmpW has been implicated in the adaptation to stresses in various species. It is recently found to be present in the regulon of the global anaerobic transcription factor FNR and ArcA in E. coli. However, little is known about the physiological implications of this regulatory disposition. In this study, we demonstrate that transcription of ompW is indeed mediated by a series of global regulators involved in the anaerobiosis of E. coli. We show that FNR can both activate and repress the expression of ompW through its direct binding to two distinctive sites, -81.5 and -126.5 bp respectively, on ompW promoter. ArcA also participates in repression of ompW under anaerobic condition, but in an FNR dependent manner. Additionally, ompW is also subject to the regulation by CRP and NarL which senses the availability and types of carbon sources and respiration electron acceptors in the environment respectively, implying a role of OmpW in the carbon and energy metabolism of E. coli during its anaerobic adaptation. Molecular docking reveals that OmpW can bind fumarate, an alternative electron acceptor in anaerobic respiration, with sufficient affinity. Moreover, supplement of fumarate or succinate which belongs to the C4-dicarboxylates family of metabolite, to E. coli culture rescues OmpW-mediated colicin S4 killing. Taken together, we propose that OmpW is involved in anaerobic carbon and energy metabolism to mediate the transition from aerobic to anaerobic lifestyle in E. coli.

  11. Transcription factors FabR and FadR regulate both aerobic and anaerobic pathways for unsaturated fatty acid biosynthesis in Shewanella oneidensis

    Directory of Open Access Journals (Sweden)

    Qixia eLuo

    2014-12-01

    Full Text Available As genes for type II fatty acid synthesis are essential to the growth of Escherichia coli, its sole (anaerobic pathway has significant potential as a target for novel antibacterial drug, and has been extensively studied. Despite this, we still know surprisingly little about fatty acid synthesis in bacteria because this anaerobic pathway in fact is not widely distributed. In this study, we show a novel model of unsaturated fatty acid (UFA synthesis in Shewanella, emerging human pathogens in addition to well-known metal reducers. We identify both anaerobic and aerobic UFA biosynthesis pathways in the representative species, S. oneidensis. Uniquely, the bacterium also contains two regulators FabR and FadR, whose counterparts in other bacteria control the anaerobic pathway. However, we show that in S. oneidensis these two regulators are involved in regulation of both pathways, in either direct or indirect manner. Overall, our results indicate that the UFA biosynthesis and its regulation are far more complex than previously expected, and S. oneidensis serves as a good research model for further work.

  12. Anaerobic/aerobic treatment for the waste water from the Instituto de Investigaciones Electricas installations; Tratamiento anaerobio-aerobio de las aguas residuales de las instalaciones del IIE

    Energy Technology Data Exchange (ETDEWEB)

    Arvizu Fernandez, Jose Luis [Instituto de Investigaciones Electricas, Cuernavaca (Mexico)

    1996-12-31

    The waste water treatment plant of the Instituto de Investigaciones Electricas (IIE) is analyzed. This plant originally was a plant of the activated sludge type (aerobic development), process that consumes large amounts of energy and that produces residual sludge 5 or 6 times greater than the anaerobic processes. The methodology and the techniques utilized were base in the analysis of the existing plant introducing an anaerobic treatment and performing laboratory experimentation. The plant design parameters considered are described. The results show the benefits of treating by anaerobic means the waste waters since depuration are obtained on the order of 75%, as well as the reduction of resulting residual sludge and in energy consumption. A reduction of 60% to 70% can be attained if a combination of anaerobic and aerobic processes is utilized [Espanol] Se analiza la planta de tratamiento de aguas residuales del Instituto de Investigaciones Electricas (IIE). Esta planta era originalmente una planta de lodos activados (desarrollo aerobio), proceso que consume mucha energia y genera lodos residuales de 5 a 6 veces mas que los procesos anaerobios. La metodologia y las tecnicas utilizadas se basaron en el analisis de la planta de tratamiento existente en el IIE; en la caracterizacion residual del IIE; en la modificacion de la planta de tratamiento introduciendo un tratamiento anaerobio y efectuando experimentacion en laboratorio. Se describen los parametros de diseno de la planta considerados. Los resultados muestran los beneficios de tratar por medios anaerobios las aguas residuales ya que se obtienen depuraciones del orden del 75%, asi como la reduccion de lodos residuales resultantes y el consumo de energia, pueden reducirse de un 60% a un 70% si se utiliza la combinacion de los procesos anaerobios y aerobios

  13. Comprehensive analysis of glucose and xylose metabolism in Escherichia coli under aerobic and anaerobic conditions by (13)C metabolic flux analysis.

    Science.gov (United States)

    Gonzalez, Jacqueline E; Long, Christopher P; Antoniewicz, Maciek R

    2017-01-01

    Glucose and xylose are the two most abundant sugars derived from the breakdown of lignocellulosic biomass. While aerobic glucose metabolism is relatively well understood in E. coli, until now there have been only a handful of studies focused on anaerobic glucose metabolism and no (13)C-flux studies on xylose metabolism. In the absence of experimentally validated flux maps, constraint-based approaches such as MOMA and RELATCH cannot be used to guide new metabolic engineering designs. In this work, we have addressed this critical gap in current understanding by performing comprehensive characterizations of glucose and xylose metabolism under aerobic and anaerobic conditions, using recent state-of-the-art techniques in (13)C metabolic flux analysis ((13)C-MFA). Specifically, we quantified precise metabolic fluxes for each condition by performing parallel labeling experiments and analyzing the data through integrated (13)C-MFA using the optimal tracers [1,2-(13)C]glucose, [1,6-(13)C]glucose, [1,2-(13)C]xylose and [5-(13)C]xylose. We also quantified changes in biomass composition and confirmed turnover of macromolecules by applying [U-(13)C]glucose and [U-(13)C]xylose tracers. We demonstrated that under anaerobic growth conditions there is significant turnover of lipids and that a significant portion of CO2 originates from biomass turnover. Using knockout strains, we also demonstrated that β-oxidation is critical for anaerobic growth on xylose. Quantitative analysis of co-factor balances (NADH/FADH2, NADPH, and ATP) for different growth conditions provided new insights regarding the interplay of energy and redox metabolism and the impact on E. coli cell physiology.

  14. The Analysis of a Microbial Community in the UV/O3-Anaerobic/Aerobic Integrated Process for Petrochemical Nanofiltration Concentrate (NFC Treatment by 454-Pyrosequencing.

    Directory of Open Access Journals (Sweden)

    Chao Wei

    Full Text Available In this study, high-throughput pyrosequencing was applied on the analysis of the microbial community of activated sludge and biofilm in a lab-scale UV/O3- anaerobic/aerobic (A/O integrated process for the treatment of petrochemical nanofiltration concentrate (NFC wastewater. NFC is a type of saline wastewater with low biodegradability. From the anaerobic activated sludge (Sample A and aerobic biofilm (Sample O, 59,748 and 51,231 valid sequence reads were obtained, respectively. The dominant phylotypes related to the metabolism of organic compounds, polycyclic aromatic hydrocarbon (PAH biodegradation, assimilation of carbon from benzene, and the biodegradation of nitrogenous organic compounds were detected as genus Clostridium, genera Pseudomonas and Stenotrophomonas, class Betaproteobacteria, and genus Hyphomicrobium. Furthermore, the nitrite-oxidising bacteria Nitrospira, nitrite-reducing and sulphate-oxidising bacteria (NR-SRB Thioalkalivibrio were also detected. In the last twenty operational days, the total Chemical Oxygen Demand (COD and Total Organic Carbon (TOC removal efficiencies on average were 64.93% and 62.06%, respectively. The removal efficiencies of ammonia nitrogen and Total Nitrogen (TN on average were 90.51% and 75.11% during the entire treatment process.

  15. Biodegradation of 2,3,7,8 TCDD by anaerobic and aerobic microcosms collected from bioremediation treatments for cleaning up dioxin contaminated soils

    Energy Technology Data Exchange (ETDEWEB)

    Ha, Dang Thi; Tuan, Mai Anh; Viet, Nguyen Quoc; Sanh, Nguyen Thi [Vietnamese Academy of Science and Technology (VAST) (Viet Nam). Inst. of Biotechnology; Sau, Trinh Khac [Vietnam-Russian Tropical Center (Viet Nam); Papke, O. [ERGO Forschungsgesellschaft, Hamburg (Germany)

    2004-09-15

    There are many microbes that can degrade polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurants (PCDFs) and polychlorinated biphenyls (PCBs) have been isolated including purified bacteria, actinomycetes, white rods, filamentous fungi, anaerobes and also anaerobic and aerobic consortia. Bioremediation one of biological remediation has been studied as hopeful alternative to physical and chemical treatments that using for cleaning up PCDDs, PCDFs. In Vietnam for cleaning up ''hot spot'' of some former military air bases, bioremediation has been studying in different scales of Danang site. After 18 to 24 month treatments, the reduction of toxicity was significally detected. In order to study biodegradability by different groups and one of dominated strain that are existing microorganisms in our treatments, the investigation of 2,3,7,8 TCDD anaerobic and aerobic degradations was carried out in the laboratory condition. Anaerobic microbial consortium containing three different bacteria such as two Gram- negative vibrio and rod and one gram positive cocoides bacteria. This consortium could degrade 118 pg TEQ/ml 2,3,7,8 TCDD after 133 days under sulfate reduction. Concentration of 2,3,7,8 TCDD in the soil extract that adding to medium at starting point of cultivation was 144.6 pg TEQ/ml. About 81% toxicity was removed. Aerobic consortium containing all three Gram-negative bacteria and one fungal strain. After 9 day shaking at 180 rpm/min and 30 C, 85.6 % of 164.45 pg TEQ/ml 2,3,7,8 TCDD was removed. Other preliminary results of study of 2,3,7,8 TCDD biodegradation as sole carbon and energy by show that this strain FDN30 could remove 43,45 pg TEQ/ml (59%) of 73,1 pgTEQ/ml adding dioxin after two weeks. These findings explain why high concentration of contaminants in treated soil was decreased after two year treatment. Indigenous microorganisms play leading role in the detoxification of 2,3,7,8 TCDD in contaminated soils.

  16. Low-O₂ acclimation shifts the hypoxia avoidance behaviour of snapper (Pagrus auratus) with only subtle changes in aerobic and anaerobic function.

    Science.gov (United States)

    Cook, Denham G; Iftikar, Fathima I; Baker, Daniel W; Hickey, Anthony J R; Herbert, Neill A

    2013-02-01

    It was hypothesised that chronic hypoxia acclimation (preconditioning) would alter the behavioural low-O(2) avoidance strategy of fish as a result of both aerobic and anaerobic physiological adaptations. Avoidance and physiological responses of juvenile snapper (Pagrus auratus) were therefore investigated following a 6 week period of moderate hypoxia exposure (10.2-12.1 kPa P(O(2)), 21 ± 1 °C) and compared with those of normoxic controls (P(O(2))=20-21 kPa, 21 ± 1 °C). The critical oxygen pressure (P(crit)) limit of both groups was unchanged at ~7 kPa, as were standard, routine and maximum metabolic rates. However, hypoxia-acclimated fish showed increased tolerances to hypoxia in behavioural choice chambers by avoiding lower P(O(2)) levels (3.3 ± 0.7 vs 5.3 ± 1.1 kPa) without displaying greater perturbations of lactate or glucose. This behavioural change was associated with unexpected physiological adjustments. For example, a decrease in blood O(2) carrying capacity was observed after hypoxia acclimation. Also unexpected was an increase in whole-blood P(50) following acclimation to low O(2), perhaps facilitating Hb-O(2) off-loading to tissues. In addition, cardiac mitochondria measured in situ using permeabilised fibres showed improved O(2) uptake efficiencies. The proportion of the anaerobic enzyme lactate dehydrogenase, at least relative to the aerobic marker enzyme citrate synthase, also increased in heart and skeletal red muscle, indicating enhanced anaerobic potential, or in situ lactate metabolism, in these tissues. Overall, these data suggest that a prioritization of O(2) delivery and O(2) utilisation over O(2) uptake during long-term hypoxia may convey a significant survival benefit to snapper in terms of behavioural low-O(2) tolerance.

  17. Effects of growth hormone and testosterone therapy on aerobic and anaerobic fitness , body composition and lipoprotein profile in middle-aged men

    Directory of Open Access Journals (Sweden)

    Adam Zając

    2014-03-01

    Full Text Available Introduction. Andropause and aging are associated with neuroendocrine dysfunctions. Growth hormone and testosterone play a significant role in several processes affecting adaptation and thereby also everyday functioning. The aim of this research project was to evaluate the effects of recombinant human growth hormone and testosterone enanthate injections on body mass and body composition, aerobic and anaerobic fitness and lipid profile in middle-aged men. Materials and method. The research group was comprised of 14 men aged 45 – 60 years. Two series of laboratory analyses were performed. Independent tests were carried out at baseline and after 12 weeks of the experiment. The data were analyzed using Statistica 9.1 software. Results. A two-way repeated measures ANOVA revealed a statistically significant effect of the intervention programme on fat-free mass (η2=0.34, total body fat (η2=0.79, total cholesterol (η2=0.30, high-density lipoprotein cholesterol (η2=0.31, low-density lipoprotein cholesterol (η2=0.42, triglyceride (η2=0.28, testosterone (η2=0.52, insulin-like growth factor 1 (η2=0.47 and growth hormone (η2=0.63. Furthermore, ANOVA revealed a statistically significant effect of the rhGH and T treatment on maximal oxygen uptake (η2=0.63, anaerobic threshold (η2=0.61 and maximal work rate (η2=0.53. Conclusion. It should be emphasized that the lipid profile was affected not only by rhGH+T replacement therapy, but also by the prescribed physical activity programme. The strength and endurance fitness programme alone did not cause significant changes in body mass and composition, nor the anaerobic and aerobic capacity. On the other hand, the rhGH=T treatment stimulated these changes significantly.

  18. Data Pre-Processing Method to Remove Interference of Gas Bubbles and Cell Clusters During Anaerobic and Aerobic Yeast Fermentations in a Stirred Tank Bioreactor

    Science.gov (United States)

    Princz, S.; Wenzel, U.; Miller, R.; Hessling, M.

    2014-11-01

    One aerobic and four anaerobic batch fermentations of the yeast Saccharomyces cerevisiae were conducted in a stirred bioreactor and monitored inline by NIR spectroscopy and a transflectance dip probe. From the acquired NIR spectra, chemometric partial least squares regression (PLSR) models for predicting biomass, glucose and ethanol were constructed. The spectra were directly measured in the fermentation broth and successfully inspected for adulteration using our novel data pre-processing method. These adulterations manifested as strong fluctuations in the shape and offset of the absorption spectra. They resulted from cells, cell clusters, or gas bubbles intercepting the optical path of the dip probe. In the proposed data pre-processing method, adulterated signals are removed by passing the time-scanned non-averaged spectra through two filter algorithms with a 5% quantile cutoff. The filtered spectra containing meaningful data are then averaged. A second step checks whether the whole time scan is analyzable. If true, the average is calculated and used to prepare the PLSR models. This new method distinctly improved the prediction results. To dissociate possible correlations between analyte concentrations, such as glucose and ethanol, the feeding analytes were alternately supplied at different concentrations (spiking) at the end of the four anaerobic fermentations. This procedure yielded low-error (anaerobic) PLSR models for predicting analyte concentrations of 0.31 g/l for biomass, 3.41 g/l for glucose, and 2.17 g/l for ethanol. The maximum concentrations were 14 g/l biomass, 167 g/l glucose, and 80 g/l ethanol. Data from the aerobic fermentation, carried out under high agitation and high aeration, were incorporated to realize combined PLSR models, which have not been previously reported to our knowledge.

  19. Effect of aerobic pre-treatment on hydrogen and methane production in a two-stage anaerobic digestion process using food waste with different compositions.

    Science.gov (United States)

    Rafieenia, Razieh; Girotto, Francesca; Peng, Wei; Cossu, Raffaello; Pivato, Alberto; Raga, Roberto; Lavagnolo, Maria Cristina

    2017-01-01

    Aerobic pre-treatment was applied prior to two-stage anaerobic digestion process. Three different food wastes samples, namely carbohydrate rich, protein rich and lipid rich, were prepared as substrates. Effect of aerobic pre-treatment on hydrogen and methane production was studied. Pre-aeration of substrates showed no positive impact on hydrogen production in the first stage. All three categories of pre-aerated food wastes produced less hydrogen compared to samples without pre-aeration. In the second stage, methane production increased for aerated protein rich and carbohydrate rich samples. In addition, the lag phase for carbohydrate rich substrate was shorter for aerated samples. Aerated protein rich substrate yielded the best results among substrates for methane production, with a cumulative production of approximately 351ml/gVS. With regard to non-aerated substrates, lipid rich was the best substrate for CH4 production (263ml/gVS). Pre-aerated P substrate was the best in terms of total energy generation which amounted to 9.64kJ/gVS. This study revealed aerobic pre-treatment to be a promising option for use in achieving enhanced substrate conversion efficiencies and CH4 production in a two-stage AD process, particularly when the substrate contains high amounts of proteins.

  20. Stoke's and anti-Stoke's characteristics of anaerobic and aerobic bacterias at excitation of fluorescence by low-intensity red light: I. Research of anaerobic bacterias

    Science.gov (United States)

    Masychev, Victor I.; Alexandrov, Michail T.

    2000-04-01

    Biopsy or photo dynamic therapy of tumors are usually investigated by fluorescent diagnostics methods. Information on modified method of fluorescence diagnostics of inflammatory diseases is represented in this research. Anaerobic micro organisms are often the cause of these pathological processes. These micro organisms also accompany disbiotic processes in intestines.

  1. Molecular analysis of the biomass of a fluidized bed reactor treating synthetic vinasse at anaerobic and micro-aerobic conditions

    NARCIS (Netherlands)

    Rodriguez, E.; Lopes, A.; Fdz-Polanco, M.; Stams, A.J.M.; Garcia Encina, P.A.

    2012-01-01

    The microbial communities (Bacteria and Archaea) established in an anaerobic fluidized bed reactor used to treat synthetic vinasse (betaine, glucose, acetate, propionate, and butyrate) were characterized by denaturing gradient gel electrophoresis (DGGE) and phylogenetic analysis. This study was focu

  2. Applications of aerobic and anaerobic bacteria in the fields of biological degradation of contaminants and biological wastewater treatment

    OpenAIRE

    Jovcic, Alexander

    2003-01-01

    In the work here presented four distinctly different problems were investigated. The first problem was an investigation into the degradation of Dichloroethylene (DCE) and 1,1-bis (p-Chlorophenyl)-2-dichloroethylene (DDE) utilising pure bacterial cultures. The second investigation dealt with the degradation of DDE and polychlorinated Biphenyl’s (PCB’s) utilising anaerobic sediments and soils from New Zealand. The third investigation worked on the Granulation of anaerobic River-sediments in Upf...

  3. Field-based and laboratory stable isotope probing surveys of the identities of both aerobic and anaerobic benzene-metabolizing microorganisms in freshwater sediment.

    Science.gov (United States)

    Liou, J S-C; Derito, C M; Madsen, E L

    2008-08-01

    Laboratory incubations of coal-tar waste-contaminated sediment microbial communities under relatively controlled physiological conditions were used to interpret results of a field-based stable isotope probing (SIP) assay. Biodegradation activity of 13C-benzene was examined by GC/MS determination of net 13CO2 production and by GC headspace analysis of benzene loss. Key experimental variables were: the site of the assays (laboratory serum-bottle incubations and in situ field sediments), benzene concentration (10, 36 or 200 p.p.m. in laboratory assays), and physiological conditions (anaerobic with or without sulfate or nitrate additions versus aerobic headspace or the uncontrolled field). In anaerobic laboratory incubations of benzene at 10 p.p.m., greater than 60% of the substrate was eliminated within 15 days. During anaerobic incubations of 200 p.p.m. benzene (70 days), 0.9% benzene mineralization occurred. When benzene (36 p.p.m.) was added to sediment with air in the serum-bottle headspace, 14% of the initial 13C was mineralized to 13CO2 in 2.5 days. In the field experiment (178 microg 13C-benzene dosed to undisturbed sediments), net 13CO2 production reached 0.3% within 8.5 h. After isopycnic separation of 13C (heavy)-labelled DNA from the above biodegradation assays, sequencing of 13C-DNA clone libraries revealed a broad diversity of taxa involved in benzene metabolism and distinctive libraries for each biodegradation treatment. Perhaps most importantly, in the field SIP experiment the clone libraries produced were dominated by Pelomonas (betaproteobacteria) sequences similar to those found in the anaerobic 10 p.p.m. benzene laboratory experiment. These data indicate that the physiological conditions that prevail and govern in situ biodegradation of pollutants in the field may be interpreted by knowing the physiological preferences of potentially active populations.

  4. Influence of aerobic and anaerobic conditions on survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in Luria-Bertani broth, farm-yard manure and slurry

    NARCIS (Netherlands)

    Semenov, A.V.; Overbeek, van L.S.; Termorshuizen, A.J.; Bruggen, van A.H.C.

    2011-01-01

    The influence of aerobic and anaerobic conditions on the survival of the enteropathogens Escherichia coli O157:H7 and Salmonella serovar Typhimurium was investigated in microcosms with broth, cattle manure or slurry. These substrates were inoculated with a green fluorescent protein transformed strai

  5. Influence of aerobic and anaerobic conditions on survival of Escherichia coli O157 : H7 and Salmonella enterica serovar Typhimurium in Luria-Bertani broth, farm-yard manure and slurry

    NARCIS (Netherlands)

    Semenov, Alexander V.; van Overbeek, Leo; Termorshuizen, Aad J.; van Bruggen, Ariena H. C.

    2011-01-01

    The influence of aerobic and anaerobic conditions on the survival of the enteropathogens Escherichia coli O157:H7 and Salmonella serovar Typhimurium was investigated in microcosms with broth, cattle manure or slurry. These substrates were inoculated with a green fluorescent protein transformed strai

  6. 需氧与厌氧配对培养在提高血培养阳性率中的优势%Advantages of aerobic and anaerobic paired culture on raising positive rate of blood culture

    Institute of Scientific and Technical Information of China (English)

    蒋伟燕; 李方去; 杨锦红; 王大选; 刘彩霞

    2011-01-01

    目的 探讨同时做需氧与厌氧配对培养在提高血培养阳性率中的优势.方法 在每例患者的不同部位同时抽取两份血液标本,一份注人需氧血培养瓶,一份注入厌氧血培养瓶,两者分别采用Bact/Alert3D培养仪和BACTEC9120培养仪进行培养.结果 在3605份血培养中阳性共347份,阳性率为9.62%,其中仅需氧血培养阳性120份,占34.58%,仅厌氧血培养阳性88份,占25.36%,需氧与厌氧血培养均阳性139份,占40.06%;在88份仅厌氧血培养阳性中检出厌氧菌3份,占总阳性份数的0.86%.结论 同时做需氧厌氧配对培养能够提高血培养的阳性率,常规开展厌氧血培养十分必要.%OBJECTIVE To discuss the advantage of aerobic and anaerobic paired culture on raising the positive rate of blood culture. METHODS Two samples were collected from each patients different sites at the same time, one was injected into an aerobic blood culture bottle,and the other was injected into an anaerobic blood culture bottle. The aerobic and anaerobic blood culture bottles were cultured by using Baet/Alert 3D cultivator and BACTEC 9120 cultivator respectively. RESULTS There were 347 isolates(9. 62%) recovered from 3605 paired aerobic and anaerobic blood culture bottles, 120 positive isolates (34. 58 %) recovered only from aerobic bottles, and 88 isolates (25. 36 %) recovered only from anaerobic bottles , 139 isolates (40.06 % ) recovered from both of the two bottles. 3 cases of anaerobic bacteria were recovered in 88 isolates, accounting for 0.86%. CONCLUSION Using paired aerobic and anaerobic blood culture bottles can raise the positive rate of blood culture. It is necessary to carry out anaerobic blood culture generally..

  7. A model‐driven quantitative metabolomics analysis of aerobic and anaerobic metabolism in E. coli K‐12 MG1655 that is biochemically and thermodynamically consistent

    DEFF Research Database (Denmark)

    McCloskey, Douglas; Gangoiti, Jon A.; King, Zachary A.

    2014-01-01

    then integrated with the E. coli genome‐scale metabolic model (GEM) via a sensitivity analysis that utilized reaction thermodynamics to reconcile simulated growth rates and reaction directionalities. This analysis highlighted several optimal network usage inconsistencies, including the incorrect use of the beta......The advent of model‐enabled workflows in systems biology allows for the integration of experimental data types with genome‐scale models to discover new features of biology. This work demonstrates such a workflow, aimed at establishing a metabolomics platform applied to study the differences...... in metabolomes between anaerobic and aerobic growth of Escherichia coli. Constraint‐based modeling was utilized to deduce a target list of compounds for downstream method development. An analytical and experimental methodology was developed and tailored to the compound chemistry and growth conditions of interest...

  8. Microbial community structure in a thermophilic aerobic digester used as a sludge pretreatment process for the mesophilic anaerobic digestion and the enhancement of methane production.

    Science.gov (United States)

    Jang, Hyun Min; Park, Sang Kyu; Ha, Jeong Hyub; Park, Jong Moon

    2013-10-01

    An effective two-stage sewage sludge digestion process, consisting of thermophilic aerobic digestion (TAD) followed by mesophilic anaerobic digestion (MAD), was developed for efficient sludge reduction and methane production. Using TAD as a biological pretreatment, the total volatile suspended solid reduction (VSSR) and methane production rate (MPR) in the MAD reactor were significantly improved. According to denaturing gradient gel electrophoresis (DGGE) analysis, the results indicated that the dominant bacteria species such as Ureibacillus thermophiles and Bacterium thermus in TAD were major routes for enhancing soluble organic matter. TAD pretreatment using a relatively short SRT of 1 day showed highly increased soluble organic products and positively affected an increment of bacteria populations which performed interrelated microbial metabolisms with methanogenic species in the MAD; consequently, a quantitative real-time PCR indicated greatly increased Methanosarcinales (acetate-utilizing methanogens) in the MAD, resulting in enhanced methane production.

  9. Microbial community structure and diversity in an integrated system of anaerobic-aerobic reactors and a constructed wetland for the treatment of tannery wastewater in Modjo, Ethiopia.

    Directory of Open Access Journals (Sweden)

    Adey Feleke Desta

    Full Text Available A culture-independent approach was used to elucidate the microbial diversity and structure in the anaerobic-aerobic reactors integrated with a constructed wetland for the treatment of tannery wastewater in Modjo town, Ethiopia. The system has been running with removal efficiencies ranging from 94%-96% for COD, 91%-100% for SO4(2- and S(2-, 92%-94% for BOD, 56%-82% for total Nitrogen and 2%-90% for NH3-N. 16S rRNA gene clone libraries were constructed and microbial community assemblies were determined by analysis of a total of 801 unique clone sequences from all the sites. Operational Taxonomic Unit (OTU--based analysis of the sequences revealed highly diverse communities in each of the reactors and the constructed wetland. A total of 32 phylotypes were identified with the dominant members affiliated to Clostridia (33%, Betaproteobacteria (10%, Bacteroidia (10%, Deltaproteobacteria (9% and Gammaproteobacteria (6%. Sequences affiliated to the class Clostridia were the most abundant across all sites. The 801 sequences were assigned to 255 OTUs, of which 3 OTUs were shared among the clone libraries from all sites. The shared OTUs comprised 80 sequences belonging to Clostridiales Family XIII Incertae Sedis, Bacteroidetes and unclassified bacterial group. Significantly different communities were harbored by the anaerobic, aerobic and rhizosphere sites of the constructed wetland. Numerous representative genera of the dominant bacterial classes obtained from the different sample sites of the integrated system have been implicated in the removal of various carbon- containing pollutants of natural and synthetic origins. To our knowledge, this is the first report of microbial community structure in tannery wastewater treatment plant from Ethiopia.

  10. The effects of salinization on aerobic and anaerobic decomposition and mineralization in peat meadows: the roles of peat type and land use.

    Science.gov (United States)

    Brouns, Karlijn; Verhoeven, Jos T A; Hefting, Mariet M

    2014-10-01

    Peat soils comprise a large part of the western and northern Netherlands. Drainage for agriculture has caused increased soil aeration which has stimulated decomposition and, hence, soil subsidence, currently amounting to 1-2 cm/yr. River water is supplied to these peat areas in summer to prevent drying out of the peat soils. Saltwater intrusion and evaporation make this surface water slightly brackish during drought periods. In addition, brackish seepage can surface more easily during such dry periods. We performed an incubation experiment in which the effects of salinization on aerobic decomposition and mineralization of shallow peat samples and anaerobic decomposition and mineralization of deep peat samples were studied. We considered four different types of peat samples: peat sampled in agricultural peat meadows and in nature reserves, originally formed under either eutrophic or oligotrophic conditions. The aerobic decomposition was approximately reduced by 50% after salinization, whereas the anaerobic decomposition rates remained unchanged. Remarkably, the response to salinization did not differ between the peat types and land uses. Ammonium concentrations increased while nitrate concentrations decreased after salinization, probably as a result of reduced nitrification. Especially in the oligotrophic peat, ammonium concentrations increased substantially. Phosphate concentrations increased, possibly caused by changes in desorption and adsorption processes due to higher ion concentrations. DOC concentrations decreased in the brackish samples due to precipitation. Furthermore, the eutrophic peat samples showed increasing sulfate concentrations, both in oxic and anoxic incubations, which was attributed to pyrite oxidation. Independently of salinization, nitrification rates were higher in the agricultural, fertilized, peat soils. In conclusion, while salinization might reduce subsidence rates, it will have adverse effects on water quality.

  11. Treatment of high salt oxidized modified starch waste water using micro-electrolysis, two-phase anaerobic aerobic and electrolysis for reuse

    Science.gov (United States)

    Yi, Xuenong; Wang, Yulin

    2016-08-01

    A combined process of micro-electrolysis, two-phase anaerobic, aerobic and electrolysis was investigated for the treatment of oxidized modified starch wastewater (OMSW). Optimum ranges for important operating variables were experimentally determined and the treated water was tested for reuse in the production process of corn starch. The optimum hydraulic retention time (HRT) of micro-electrolysis, methanation reactor, aerobic process and electrolysis process were 5, 24, 12 and 3 h, respectively. The addition of iron-carbon fillers to the acidification reactor was 200 mg/L while the best current density of electrolysis was 300 A/m2. The biodegradability was improved from 0.12 to 0.34 by micro-electrolysis. The whole treatment was found to be effective with removal of 96 % of the chemical oxygen demand (COD), 0.71 L/day of methane energy recovery. In addition, active chlorine production (15,720 mg/L) was obtained by electrolysis. The advantage of this hybrid process is that, through appropriate control of reaction conditions, effect from high concentration of salt on the treatment was avoided. Moreover, the process also produced the material needed in the production of oxidized starch while remaining emission-free and solved the problem of high process cost.

  12. Experimental otitis media in gerbils and chinchillas with Streptococcus pneumoniae, Haemophilus influenzae, and other aerobic and anaerobic bacteria.

    OpenAIRE

    Fulghum, R S; Brinn, J E; Smith, A M; Daniel, H J; Loesche, P J

    1982-01-01

    To ascertain the usefulness of Mongolian gerbils as an inbred model for otitis media, 52 Mongolian gerbils (Meriones unguiculatus, strain MONT/Tum) were compared with 26 chinchillas (Chinchilla laniger) for susceptibility to Streptococcus pneumoniae type 3. Haemophilus influenzae type b, and a polymicrobic culture including anaerobes (Streptococcus intermedius, Propionibacterium acnes, Staphylococcus epidermidis, and Corynebacterium sp.). Organisms were inoculated percutaneously into the supe...

  13. Removal of pollutants from pulp and paper mill effluent by anaerobic and aerobic treatment in pilot scale bioreactor

    DEFF Research Database (Denmark)

    Singh, P.; Katiyar, D.; Gupta, M.

    2011-01-01

    . The anaerobically treated effluent was then treated in a bioreactor in the presence of a fungal strain (Aspergillus fumigatus) or a bacterial strain (Pseudomonas ovalis). The results of this study indicated a reduction in colour (76% and 56%), lignin (78% and 68%), COD (85% and 78%) and AOX (70% and 82...

  14. Biological Nutrient Removal in a Full Scale Anoxic/Anaerobic/Aerobic/Pre-anoxic-MBR Plant for Low C/N Ratio Municipal Wastewater Treatment

    Institute of Scientific and Technical Information of China (English)

    胡香; 谢丽; 张善发; 杨殿海

    2014-01-01

    A novel full scale modified A2O (anoxic/anaerobic/aerobic/pre-anoxic)-membrane bioreactor (MBR) plant combined with the step feed strategy was operated to improve the biological nutrient removal (BNR) from low C/N ratio municipal wastewater in Southern China. Transformation of organic carbon, nitrogen and phosphorus, and membrane fouling were investigated. Experimental results for over four months demonstrated good efficiencies for chemical oxygen demand (COD) and 4NH+-N removal, with average values higher than 84.5%and 98.1%, re-spectively. A relatively higher total nitrogen (TN) removal efficiency (52.1%) was also obtained at low C/N ratio of 3.82, contributed by the configuration modification (anoxic zone before anaerobic zone) and the step feed with a distribution ratio of 1︰1. Addition of sodium acetate into the anoxic zone as the external carbon source, with a theoretical amount of 31.3 mg COD per liter in influent, enhanced denitrification and the TN removal efficiency in-creased to 74.9%. Moreover, the total phosphate (TP) removal efficiency increased by 18.0%. It is suggested that the external carbon source is needed to improve the BNR performance in treating low C/N ratio municipal waste-water in the modified A2O-MBR process.

  15. Process and device for anaerobic removal of organic substrates. Verfahren und Vorrichtung zum anaeroben Abbau organischer Substrate

    Energy Technology Data Exchange (ETDEWEB)

    Reimann, H.

    1984-09-06

    In a process for the anaerobic removal of organic substrates, these are treated in two separate anaerobic reactors 1 and 2. One anaerobic reactor 1 is used as an acid-forming reactor, while the other anaerobic reactor 2 is made as a reactor for forming acetates and methane with a biogas outflow pipe. A drain 3 is used to take partly treated organic substrate from the acid-forming reactor to the reactor forming the acetate and methane. In order to reduce or exclude the proportion of CO/sub 2/ in biogas pipe 5 in a simple and economical way, carbondioxide is removed from drain 3 of the acid-forming reactor 1. The removal of the carbondioxide is preferably done in a stripping device 6 situated in drain 3, to which an air intake pipe 7 and an air outlet pipe 8 are connected.

  16. The effects of a pre-workout supplement containing caffeine, creatine, and amino acids during three weeks of high-intensity exercise on aerobic and anaerobic performance

    Directory of Open Access Journals (Sweden)

    Kendall Kristina L

    2010-02-01

    Full Text Available Abstract Background A randomized, single-blinded, placebo-controlled, parallel design study was used to examine the effects of a pre-workout supplement combined with three weeks of high-intensity interval training (HIIT on aerobic and anaerobic running performance, training volume, and body composition. Methods Twenty-four moderately-trained recreational athletes (mean ± SD age = 21.1 ± 1.9 yrs; stature = 172.2 ± 8.7 cm; body mass = 66.2 ± 11.8 kg, VO2max = 3.21 ± 0.85 l·min-1, percent body fat = 19.0 ± 7.1% were assigned to either the active supplement (GT, n = 13 or placebo (PL, n = 11 group. The active supplement (Game Time®, Corr-Jensen Laboratories Inc., Aurora, CO was 18 g of powder, 40 kcals, and consisted of a proprietary blend including whey protein, cordyceps sinensis, creatine, citrulline, ginseng, and caffeine. The PL was also 18 g of powder, 40 kcals, and consisted of only maltodextrin, natural and artificial flavors and colors. Thirty minutes prior to all testing and training sessions, participants consumed their respective supplements mixed with 8-10 oz of water. Both groups participated in a three-week HIIT program three days per week, and testing was conducted before and after the training. Cardiovascular fitness (VO2max was assessed using open circuit spirometry (Parvo-Medics TrueOne® 2400 Metabolic Measurement System, Sandy, UT during graded exercise tests on a treadmill (Woodway, Pro Series, Waukesha, WI. Also, four high-speed runs to exhaustion were conducted at 110, 105, 100, and 90% of the treadmill velocity recorded during VO2max, and the distances achieved were plotted over the times-to-exhaustion. Linear regression was used to determine the slopes (critical velocity, CV and y-intercepts (anaerobic running capacity, ARC of these relationships to assess aerobic and anaerobic performances, respectively. Training volumes were tracked by summing the distances achieved during each training session for each subject

  17. Microbial aerobic and anaerobic degradation of acrylamide in sludge and water under environmental conditions--case study in a sand and gravel quarry.

    Science.gov (United States)

    Guezennec, A G; Michel, C; Ozturk, S; Togola, A; Guzzo, J; Desroche, N

    2015-05-01

    Polyacrylamides (PAMs) are used in sand and gravel quarries as water purification flocculants for recycling process water in a recycling loop system where the flocculants remove fine particles in the form of sludge. The PAM-based flocculants, however, contain residual amounts of acrylamide (AMD) that did not react during the polymerization process. This acrylamide is released into the environment when the sludge is discharged into a settling basin. Here, we explore the microbial diversity and the potential for AMD biodegradation in water and sludge samples collected in a quarry site submitted to low AMD concentrations. The microbial diversity, analyzed by culture-dependent methods and the denaturing gradient gel electrophoresis approach, reveals the presence of Proteobacteria, Cyanobacteria, and Actinobacteria, among which some species are known to have an AMD biodegradation activity. Results also show that the two main parts of the water recycling loop-the washing process and the settling basin-display significantly different bacterial profiles. The exposure time with residual AMD could, thus, be one of the parameters that lead to a selection of specific bacterial species. AMD degradation experiments with 0.5 g L(-1) AMD showed a high potential for biodegradation in all parts of the washing process, except the make-up water. The AMD biodegradation potential in samples collected from the washing process and settling basin was also analyzed taking into account on-site conditions: low (12 °C) and high (25 °C) temperatures reflecting the winter and summer seasons, and AMD concentrations of 50 μg L(-1). Batch tests showed rapid (as little as 18 h) AMD biodegradation under aerobic and anaerobic conditions at both the winter and summer temperatures, although there was a greater lag time before activity started with the AMD biodegradation at 12 °C. This study, thus, demonstrates that bacteria present in sludge and water samples exert an in situ and rapid

  18. Kinetics, mass transfer and hydrodynamics in a packed bed aerobic reactor fed with anaerobically treated domestic sewage.

    Science.gov (United States)

    Fazolo, A; Pasotto, M B; Foresti, E; Zaiat, M

    2006-10-01

    This study presents an assessment of the kinetic, mass transfer and hydrodynamic parameters of a pilot-scale fixed bed reactor containing immobilized biomass in polyurethane matrices and fed with the effluent of a horizontal-flow fixed bed anaerobic reactor, which was used to treat domestic sewage. It was found that the liquid-solid and intra-particle mass transfer resistances significantly affected the overall oxygen consumption rate and that mechanical agitation could minimize such resistances. The volumetric oxygen transfer coefficient (kLa) values for superficial air velocities between 8.4 cm min(-1) and 57.0 cm min(-1) varied from 20.8 h(-1) to 58.8 h(-1) for tap water, and 16.8 h(-1) to 53.0 h(-1) for the anaerobic pre-treated effluent. The intrinsic oxygen uptake rate was estimated to be 19.9 mgO2 gVSS(-1) h(-1). A first-order kinetic model with residual concentration was considered to adequately represent the COD removal rate, whereas nitrogen conversion was considered to be well represented by a model of pseudo-first-order reaction in series. It was also found that the ammonium conversion to nitrite was the limiting step of the overall nitrogen conversion rate. The hydrodynamic behavior of the reactor was represented by three to four completely mixed reactors in series.

  19. Research on Aerobic Capacity and Anaerobic Capacity of Young Men Athletes%青年男子运动员有氧及无氧能力的研究

    Institute of Scientific and Technical Information of China (English)

    鹿琦; 张立; 夏书; 周岱

    2011-01-01

    Through the test of aerobic capacity and anaerobic capacity to understand physical status of young men kayak athletes and rowers,observed their metabolism characteristics and the differences of aerobic energy and anaerobic work capacity when exercise.To provide first-hand information for the reasonable and scientific training programs.%通过对青年男子皮艇运动员与赛艇运动员的有氧能力和无氧能力的测试来了解其体能现状,观察在运动时他们的能量代谢特点及有氧和无氧工作能力的差异,为合理而科学的训练计划提供第一手资料。

  20. Sludge Retention Time as a Suitable Operational Parameter to Remove Both Estrogen and Nutrients in an Anaerobic-Anoxic-Aerobic Activated Sludge System.

    Science.gov (United States)

    Zeng, Qingling; Li, Yongmei; Yang, Shijia

    2013-04-01

    Estrogen in wastewater are responsible for a significant part of the endocrine-disrupting effects observed in the aquatic environment. The effect of sludge retention time (SRT) on the removal and fate of 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) in an anaerobic-anoxic-oxic activated sludge system designed for nutrient removal was investigated by laboratory-scale experiments using synthetic wastewater. With a hydraulic retention time of 8 h, when SRT ranged 10-25 days, E2 was almost completely removed from water, and EE2 removal efficiency was 65%-81%. Both estrogens were easily sorbed onto activated sludge. Distribution coefficients (Kd) of estrogens on anaerobic sludge were greater than those on anoxic and aerobic sludges. Mass balance calculation indicated that 99% of influent E2 was degraded by the activated sludge process, and 1% remained in excess sludge; of influent EE2, 62.0%-80.1% was biodegraded; 18.9%-34.7% was released in effluent; and 0.88%-3.31% remained in excess sludge. Optimal SRT was 20 days for both estrogen and nutrient removal. E2 was almost completely degraded, and EE2 was only partly degraded in the activated sludge process. Residual estrogen on excess sludge must be considered in the sludge treatment and disposal processes. The originality of the work is that removal of nutrients and estrogens were linked, and optimal SRT for both estrogen and nutrient removal in an enhanced biological phosphorus removal system was determined. This has an important implication for the design and operation of full-scale wastewater treatment plants.

  1. Comparison of the sensitivities of fish, Microtox and Daphnia-magna bioassays to amoxycillin in anaerobic/aerobic sequential reactor systems.

    Science.gov (United States)

    Çelebi, H; Sponza, D T

    2012-01-01

    In this study the anaerobic treatability of amoxycillin (AMX) was investigated in a laboratory-scale anaerobic multi-chamber bed reactor (AMCBR)/aerobic continuously stirred tank reactor (CSTR) system. The chemical oxygen demand (COD) and AMX removal efficiencies were around 94% in the AMCBR reactor at hydraulic retention times (HRTs) between 2.25 and 5.5 days. Decreasing the HRT appeared not to have a significant effect on the performance of the AMCBR up to a HRT of 1.13 days. The maximum methane production rate and methane percentage were around 1,100-1,200 mL/day and 55%, respectively, at HRTs between 2.25 and 5.5 days. The decrease in HRT to 1.5 days decreased slightly the gas productions (1,000 mL/day and 500 mL for total and methane gases) and methane percentage (45%). The AMCBR recovered back to its baseline performance within a couple of days. The acute toxicity of 150 mg/L AMX was monitored with Daphnia magna, Lepistes sp., and Vibrio fischeri acute toxicity tests. The acute toxicity removals were 98, 96 and 96% for V. fischeri, D. magna and Lepistes sp. in the effluent of the sequential system treating 150 mg/L AMX at HRTs of 2.25-5.5 days. Among the trophic organisms used in the acute toxicity tests the most sensitive organism was found to be bacteria (V. fischeri) while the most resistant organism was found to be fish (Lepistes sp.).

  2. Expression of the succinate dehydrogenase genes (sdhCAB) from the facultatively anaerobic paenibacillus macerans during aerobic growth

    Science.gov (United States)

    Schirawski; Hankeln; Unden

    1998-10-01

    Paenibacillus (formerly Bacillus) macerans is capable of succinate oxidation under oxic conditions and fumarate reduction under anoxic conditions. The reactions are catalyzed by different enzymes, succinate dehydrogenase (Sdh) and fumarate reductase (Frd). The genes encoding Sdh (sdhCAB) were analyzed. The gene products of sdhA and sdhB were similar to the subunits of known Sdh and Frd enzymes. The hydrophobic subunit SdhC showed close sequence similarity to the class of Sdh/Frd enzymes containing diheme cytochrome b. From the sdhCAB gene cluster two transcripts were produced, one comprising sdhCAB, the other sdhAB. The transcripts were found only during aerobic growth, and the amount was directly proportional to Sdh activity, but inversely proportional to Frd activity.

  3. Start-up of the SHARON and ANAMMOX process in landfill bioreactors using aerobic and anaerobic ammonium oxidising biomass.

    Science.gov (United States)

    Shalini, S Sri; Joseph, Kurian

    2013-12-01

    The main aim of this study is to analyse the feasibility to use aerobic ammonium oxidising bacteria (AOB) and anammox/AnAOB biomass enriched from mined municipal solid waste for in situ SHARON and ANAMMOX processes in laboratory scale landfill bioreactors (LFBR) for ammonia nitrogen removal. For this purpose, three LFBRs were operated as Control (without biomass seed), SHARON (with AOB biomass seed) and ANAMMOX (with anammox biomass seed) for 315 days. Results showed nitrogen loading rate of 1.0 kg N/d was effectively removed in SHARON and ANAMMOX LFBR. In SHARON LFBR, partial nitritation efficiency reached up to 98.5% with AOB population of MPN of 5.1 × 10(6)/mL obtained. ANAMMOX LFBR gave evolution of 95% of nitrogen gas as the end product confirmed the ANAMMOX process. Nitrogen transformations, biomass development and hydrazine and hydroxylamine formation authenticated the enriched AOB and anammox biomass activity in landfill bioreactors.

  4. High-Intensity Intermittent Training Positively Affects Aerobic and Anaerobic Performance in Judo Athletes Independently of Exercise Mode

    Science.gov (United States)

    Franchini, Emerson; Julio, Ursula F.; Panissa, Valéria L. G.; Lira, Fábio S.; Gerosa-Neto, José; Branco, Braulio H. M.

    2016-01-01

    Purpose: The present study investigated the effects of high-intensity intermittent training (HIIT) on lower- and upper-body graded exercise and high-intensity intermittent exercise (HIIE, four Wingate bouts) performance, and on physiological and muscle damage markers responses in judo athletes. Methods: Thirty-five subjects were randomly allocated to a control group (n = 8) or to one of the following HIIT groups (n = 9 for each) and tested pre- and post-four weeks (2 training d·wk−1): (1) lower-body cycle-ergometer; (2) upper-body cycle-ergometer; (3) uchi-komi (judo technique entrance). All HIIT were constituted by two blocks of 10 sets of 20 s of all out effort interspersed by 10 s set intervals and 5-min between blocks. Results: For the upper-body group there was an increase in maximal aerobic power in graded upper-body exercise test (12.3%). The lower-body group increased power at onset blood lactate in graded upper-body exercise test (22.1%). The uchi-komi group increased peak power in upper- (16.7%) and lower-body (8.5%), while the lower-body group increased lower-body mean power (14.2%) during the HIIE. There was a decrease in the delta blood lactate for the uchi-komi training group and in the third and fourth bouts for the upper-body training group. Training induced testosterone-cortisol ratio increased in the lower-body HIIE for the lower-body (14.9%) and uchi-komi (61.4%) training groups. Conclusion: Thus, short-duration low-volume HIIT added to regular judo training was able to increase upper-body aerobic power, lower- and upper-body HIIE performance. PMID:27445856

  5. 涪陵污水处理剩余污泥的好氧、厌氧两种堆肥处理比较%A Contrastive Study on Aerobic Composting and Anaerobic Composting of Sewage Sludge Retreatment in Fuling

    Institute of Scientific and Technical Information of China (English)

    陈科平; 杨琼; 杨季冬

    2014-01-01

    好氧堆肥和厌氧堆肥是较为成熟的堆肥技术,在堆肥成效上各有优劣。文章在涪陵城区污水处理厂所产污泥的成分分析的基础上,对其好氧堆肥和厌氧堆肥作了比较实验,提出一些关键性的实验参数以资后续研究参考依据。同时,探索了好氧厌氧交替堆肥实验,取得令人满意的数据成果。%Aerobic composting and anaerobic composting is a mature composting technology, and each has advantages and disadvantages in the composting results. This article analyzes the components of sludge from the sewage treatment plant in Fuling city. On the basis of the aerobic composting and anaerobic composting experiment, some key experimental parameters reference to further research is compared. The experiment also explored the alternative aerobic and anaerobic composting technology, and the data and results obtained are satisfactory.

  6. Economic and environmental sustainability of submerged anaerobic MBR-based (AnMBR-based) technology as compared to aerobic-based technologies for moderate-/high-loaded urban wastewater treatment.

    Science.gov (United States)

    Pretel, R; Robles, A; Ruano, M V; Seco, A; Ferrer, J

    2016-01-15

    The objective of this study was to assess the economic and environmental sustainability of submerged anaerobic membrane bioreactors (AnMBRs) in comparison with aerobic-based technologies for moderate-/high-loaded urban wastewater (UWW) treatment. To this aim, a combined approach of steady-state performance modelling, life cycle analysis (LCA) and life cycle costing (LCC) was used, in which AnMBR (coupled with an aerobic-based post-treatment) was compared to aerobic membrane bioreactor (AeMBR) and conventional activated sludge (CAS). AnMBR with CAS-based post-treatment for nutrient removal was identified as a sustainable option for moderate-/high-loaded UWW treatment: low energy consumption and reduced sludge production could be obtained at given operating conditions. In addition, significant reductions can be achieved in different aspects of environmental impact (global warming potential (GWP), abiotic depletion, acidification, etc.) and LCC over existing UWW treatment technologies.

  7. Correlation between Phylogroups and Intracellular Proteomes of Propionibacterium acnes and Differences in the Protein Expression Profiles between Anaerobically and Aerobically Grown Cells

    Directory of Open Access Journals (Sweden)

    Itaru Dekio

    2013-01-01

    Full Text Available Propionibacterium acnes is one of the dominant commensals on the human skin and also an opportunistic pathogen in relation to acne, sarcoidosis, prostate cancer, and various infections. Recent investigations using housekeeping and virulence genes have revealed that the species consists of three major evolutionary clades (types I, II, and III. In order to investigate protein expression differences between these phylogroups, proteomic profiles of 21 strains of P. acnes were investigated. The proteins extracted from cells cultured under anaerobic and aerobic conditions were analysed using a SELDI-TOF mass spectrometer, high-resolution capillary gel electrophoresis, and LC-MS/ MS. The SELDI spectral profiles were visualised as a heat map and a dendrogram, which resulted in four proteomic groups. Strains belonging to type I were represented in the proteome Group A, while Group B contained type III strains. Groups C and D contained mixtures of types I and II. Each of these groups was not influenced by differences in culture conditions. Under anoxic growth conditions, a type IB strain yielded high expressions of some proteins, such as methylmalonyl-CoA epimerase and the Christie-Atkins-Munch-Petersen (CAMP factor. The present study revealed good congruence between genomic and proteomic data suggesting that the microenvironment of each subtype may influence protein expression.

  8. Combined mesophilic anaerobic and thermophilic aerobic digestion process for high-strength food wastewater to increase removal efficiency and reduce sludge discharge.

    Science.gov (United States)

    Jang, H M; Park, S K; Ha, J H; Park, J M

    2014-01-01

    In this study, a process that combines the mesophilic anaerobic digestion (MAD) process with thermophilic aerobic digestion (TAD) for high-strength food wastewater (FWW) treatment was developed to examine the removal of organic matter and methane production. All effluent discharged from the MAD process was separated into solid and liquid portions. The liquid part was discarded and the sludge part was passed to the TAD process for further degradation. Then, the digested sludge from the TAD process was recycled back to the MAD unit to achieve low sludge discharge from the combined process. The reactor combination was operated in two phases: during Phase I, 40 d of total hydraulic retention time (HRT) was applied; during Phase II, 20 d was applied. HRT of the TAD process was fixed at 5 d. For a comparison, a control process (single-stage MAD) was operated with the same HRTs of the combined process. Our results indicated that the combined process showed over 90% total solids, volatile solids and chemical oxygen demand removal efficiencies. In addition, the combined process showed a significantly higher methane production rate than that of the control process. Consequently, the experimental data demonstrated that the combined MAD-TAD process was successfully employed for high-strength FWW treatment with highly efficient organic matter reduction and methane production.

  9. High rate CNP removal from a milk processing wastewater in a single ultrasound augmented up-flow anaerobic/aerobic/anoxic bioreactor.

    Science.gov (United States)

    Rezaee, S; Zinatizadeh, A A L; Asadi, A

    2015-03-01

    Simultaneous removal of carbon, nitrogen and phosphorus (CNP) in a single bioreactor is of high significance in terms of reactor volume and energy consumption. Therefore, in this study, an innovative up-flow anaerobic/aerobic/anoxic bioreactor (UAAASB) augmented by ultrasound was developed as a high rate single bioreactor for the simultaneous removal of nutrients from a milk processing wastewater. The ultrasonic irradiation used in this work was in the range of high frequency (1.7 MHz). The central composite design (CCD) and response surface methodology (RSM) were applied to design the experimental conditions, model obtained data, and optimize the process. The effects of three independent variables, i.e. hydraulic retention time (HRT), aeration mode and mixed liquor suspended solid (MLSS) concentration on 10 process responses were investigated. The results prove that the ultrasonic irradiation has a positive effect on the sludge settling velocity and effluent turbidity. The optimum conditions were determined as 12-15 h, 4000-5000 mg/l and 1.5-2 for HRT, MLSS concentration and aeration mode, respectively, based on removal efficiency of sCOD ⩾ 90%, TN and TP ⩾ 50%.

  10. Sequential anaerobic-aerobic biodegradation of 2,3,7,8-TCDD contaminated soil in the presence of CMC-coated nZVI and surfactant.

    Science.gov (United States)

    Binh, Nguyen Duy; Imsapsangworn, Chaiyaporn; Kim Oanh, Nguyen Thi; Parkpian, Preeda; Karstensen, Kare; Giao, Pham Huy; DeLaune, Ronald D

    2016-01-01

    Enriched microorganisms in sediment collected from a dioxin-contaminated site in Vietnam (Bien Hoa airbase) were used for examining the effectiveness in biological treatment of 2,3,7,8-Tetrachlorodibenzo-p-dioxin in soil. Four bio-treatments were investigated using a sequential anaerobic (17 weeks) followed by an aerobic (6 weeks) incubation. The maximum removal efficiency was approximately 60% even at an extremely low pH (approx. 3.6) condition. Surfactant Tween-80 was added to enhance the bioavailability of dioxin in two treatments, but it appeared to biostimulate methanogens rather than dechlorinators. As a result, methane production was the highest while the dioxin removal efficiency was the lowest, as compared with the other bio-treatments. Carboxymethylcellulose (CMC) coated on nanoscale zero valent iron (nZVI) surface used in two treatments could prevent the direct contact between bacterial cell surface and nZVI which prevented cell death and lysis, hence enhancing dioxin removal. The presence of CMC--_nZVI in bio-treatments gradually released H2 required for microbiological processes, but the amount used in the experiments were likely too high to maintain optimum H2 levels for biostimulating dechlorinators rather than methanogens.

  11. 血培养需氧及厌氧瓶阳性检出率与报警时间分析%Analysis of positive rate and alarm time of aerobic and anaerobic blood culture bottle

    Institute of Scientific and Technical Information of China (English)

    答嵘; 王伟; 马晨; 俱西驰

    2015-01-01

    OBJECTIVE To analyze the positive rate ,pathogenic distribution and alarm time of aerobic and anaero‐bic bottles in blood culture so as to provide clinical references for treatments .METHODS Totally 1684 sets of blood culture bottle were collected from Sep .to Nov .2013 ,and they were cultivated by BacT/ALERT 3D auto‐matic bacteria/mycobacterial culture .Identification and drug sensitive test were performed by VITEK‐2 Compact automatic microbe cultivation and drug susceptibility instrument . The data were analyzed by SPSS 18 .0 . RESULTS In this study ,1684 sets of blood culture bottle including aerobic and anaerobic bottles were collected . Totally 1498 sets were double negative ,and 101 sets were double positive .Generally 66 sets of bottle were aerobic positive and anaerobic negative ,while 19 sets of bottle were anaerobic positive and aerobic negative .Positive rate of aerobic bottle was 9 .92% ,while that of anaerobic bottles was 7 .13% .The detection rate of Acinetobacter , Brucella ,Micrococcus ,Corynebacterium and fungi in aerobic bottle was 100% ,while anaerobic bacteria in anae‐robic bottle was 100% .Alarm time of aerobic and anaerobic bottles had no statistical differences .CONCLUSION Blood culture of double sets of aerobic and anaerobic blood culture bottles is recommended ,which improves the positive rate and discriminate pollution .%目的:分析成套血培养中需氧与厌氧瓶的阳性检出率、病原菌分布与报警时间的特点,为临床诊断提供参考依据。方法收集2013年9-11月血培养瓶1684套,采用法国生物梅里埃公司BacT/ALERT 3D全自动细菌/分枝杆菌培养仪进行培养,阳性报警时转种采用VIT EK‐2 Compact全自动微生物鉴定与药敏仪进行病原菌鉴定与药敏试验,采用SPSS 18.0软件进行数据统计分析。结果收集的1684套血培养瓶中需氧瓶与厌氧瓶同时阴性1498套,同时阳性101套,需氧瓶阳性而厌氧瓶阴性66套,需

  12. Experimental Study on Treatment of Poultry Manure with High Nitrogen Content by Anaerobic-Aerobic Co-treatment%高含氮养殖污水厌氧-好氧组合处理实验研究

    Institute of Scientific and Technical Information of China (English)

    李清; 胡启春; 祝其丽; 何明雄; 潘科

    2011-01-01

    Anaerobic-aerobic co-treatment of diluted poultry manure was done in the batch anaerobic reactors and aerobic shaking bottles based on SBR concept to especially study efficiency of aerobic-post-treatment influenced by anaerobic pre-digestion with different HRT, the reason of removal of COD, NH4+-N, TN and TP was discussed. HRT of experimental anaerobic digestion was 3,6,9,12,15 and 18 days, and cycle time of aerobic process was 12 h. Time of post-treatment experiment was 30 days. Experimental results showed that poultry manure and sewage could be treated by anaerobic digestion with renewable energy benefit. For meeting the requirement both for maximum biogas production in anaerobic digestion period and digested effluent feasible treated in post aerobic-treatment period, HRT of anaerobic digestion should be less than 6days, while BOD5/COD and C/N index of effluent could just suit the requirement of post-treatment. Comparison between data with poultry manure and others with pig and caw manure indicated that the longer the HRT of anaerobic digestion, the worse of result of aerobic post-treatment. For farm wastewater with high nitrogen content, HRT of anaerobic digestion as pre-treatment period should be comparably less.%采用批次厌氧消化和实验模拟SBR法处理低浓度蛋鸡粪污水,侧重研究厌氧消化时间对好氧后处理效果的影响,同时讨论了对COD、NH4+-N、TN和TP去除原因.厌氧消化的时问分别为3d,6d,9d,12 d,15d,18 d,好氧后处理的工艺周期为12 h.好氧阶段持续时间为30 d.结果表明,鸡粪等高含氮养殖污水能够采用厌氧消化处理和资源化利用.如果要兼顾最大产沼气化与沼液可生化性之间的最适要求,厌氧消化时间不宜超过6d,此时沼液的BOD5/COD和C/N比值勉强能够适应好氧后处理条件需要.比较本实验采用蛋鸡养殖污水与采用猪场和奶牛场污水做的类似实验结果分析发现:在厌氧-好氧组合处理模式中,厌氧消化时

  13. Anaerobic Biodegradation of Detergent Surfactants

    Directory of Open Access Journals (Sweden)

    Erich Jelen

    2009-03-01

    Full Text Available Detergent surfactantscan be found in wastewater in relevant concentrations. Most of them are known as ready degradable under aerobic conditions, as required by European legislation. Far fewer surfactants have been tested so far for biodegradability under anaerobic conditions. The natural environment is predominantly aerobic, but there are some environmental compartments such as river sediments, sub-surface soil layer and anaerobic sludge digesters of wastewater treatment plants which have strictly anaerobic conditions. This review gives an overview on anaerobic biodegradation processes, the methods for testing anaerobic biodegradability, and the anaerobic biodegradability of different detergent surfactant types (anionic, nonionic, cationic, amphoteric surfactants.

  14. Adherence to abiotic surface induces SOS response in Escherichia coli K-12 strains under aerobic and anaerobic conditions.

    Science.gov (United States)

    Costa, Suelen B; Campos, Ana Carolina C; Pereira, Ana Claudia M; de Mattos-Guaraldi, Ana Luiza; Júnior, Raphael Hirata; Rosa, Ana Cláudia P; Asad, Lídia M B O

    2014-09-01

    During the colonization of surfaces, Escherichia coli bacteria often encounter DNA-damaging agents and these agents can induce several defence mechanisms. Base excision repair (BER) is dedicated to the repair of oxidative DNA damage caused by reactive oxygen species (ROS) generated by chemical and physical agents or by metabolism. In this work, we have evaluated whether the interaction with an abiotic surface by mutants derived from E. coli K-12 deficient in some enzymes that are part of BER causes DNA damage and associated filamentation. Moreover, we studied the role of endonuclease V (nfi gene; 1506 mutant strain) in biofilm formation. Endonuclease V is an enzyme that is involved in DNA repair of nitrosative lesions. We verified that endonuclease V is involved in biofilm formation. Our results showed more filamentation in the xthA mutant (BW9091) and triple xthA nfo nth mutant (BW535) than in the wild-type strain (AB1157). By contrast, the mutant nfi did not present filamentation in biofilm, although its wild-type strain (1466) showed rare filaments in biofilm. The filamentation of bacterial cells attaching to a surface was a consequence of SOS induction measured by the SOS chromotest. However, biofilm formation depended on the ability of the bacteria to induce the SOS response since the mutant lexA Ind(-) did not induce the SOS response and did not form any biofilm. Oxygen tension was an important factor for the interaction of the BER mutants, since these mutants exhibited decreased quantitative adherence under anaerobic conditions. However, our results showed that the presence or absence of oxygen did not affect the viability of BW9091 and BW535 strains. The nfi mutant and its wild-type did not exhibit decreased biofilm formation under anaerobic conditions. Scanning electron microscopy was also performed on the E. coli K-12 strains that had adhered to the glass, and we observed the presence of a structure similar to an extracellular matrix that depended on the

  15. Experimental otitis media in gerbils and chinchillas with Streptococcus pneumoniae, Haemophilus influenzae, and other aerobic and anaerobic bacteria.

    Science.gov (United States)

    Fulghum, R S; Brinn, J E; Smith, A M; Daniel, H J; Loesche, P J

    1982-05-01

    To ascertain the usefulness of Mongolian gerbils as an inbred model for otitis media, 52 Mongolian gerbils (Meriones unguiculatus, strain MONT/Tum) were compared with 26 chinchillas (Chinchilla laniger) for susceptibility to Streptococcus pneumoniae type 3. Haemophilus influenzae type b, and a polymicrobic culture including anaerobes (Streptococcus intermedius, Propionibacterium acnes, Staphylococcus epidermidis, and Corynebacterium sp.). Organisms were inoculated percutaneously into the superior chamber of the middle ear bulla. The gerbils and chinchillas shared similar susceptibilities and responses to the inoculated organisms as determined by X-ray, otoscopic, histopathological, and microbiological determinations at 5 to 7 days. Koch's postulate studies proved the role of S. pneumoniae and H. influenzae in the pathology found in both animal models. The animals were also susceptible to the polymicrobic culture, although the relative virulence of the individual members of this mixture was low, suggesting that these species potentiated as a polymicrobic mixture. The Corynebacterium sp. appeared to elicit the greatest histopathological response in chronic (8-week) studies in gerbils. The gerbils were found to be useful as an alternative animal model for the study of otitis media of bacterial etiology.

  16. Pre-exercise intake of different carbohydrates modifies ischemic reactive hyperemia after a session of anaerobic, but not after aerobic exercise.

    Science.gov (United States)

    Fernández, Juan M; Da Silva-Grigoletto, Marzo E; Caballero-Villarraso, Javier; Gómez-Puerto, José R; Viana-Montaner, Bernardo H; Tasset-Cuevas, Inmaculada; Túnez-Fiñana, Isaac; Pérez-Martínez, Pablo; López-Miranda, José; Pérez-Jiménez, Francisco

    2010-06-01

    The acute ingestion of a supplement with different glycemic carbohydrates, including fructose, is a typical practice for athletes before exercising. Observational evidence suggests that different metabolic responses may modify the exercise-stimulated endothelium-dependent vasodilation. The purpose of the present study was to investigate whether endothelial reactivity, stimulated by anaerobic exercise (AnE) or aerobic exercise (AE), both performed with glycemic supplementation, is modified by the addition of fructose. Twenty physically trained men ingested an oral dose of glucose (G) or glucose plus fructose (F) 15 minutes before starting a 30-minute session of AnE (10 sets of 10 repetitions of half squat) or AE (cycling). The combination resulted in 4 randomized interventions in a crossover design in which all subjects performed all experimental conditions: G+AnE, F+AnE, G+AE, and F+AE. Ischemic reactive hyperemia (IRH), glycemia, plasma lipoperoxides (LPOs), nitric oxide (NO), and lactate were determined at baseline, exercise, and acute recovery time points. Immediately after AnE, IRH was 26.35% higher in F+AnE than in G+AnE (p<0.05); this difference rose to 27.24% at the end of the recovery period (p<0.05). The glycemic peak in F+AnE was lower than in G+AnE (p<0.05), and there was a second peak during recovery (p<0.05). There were no differences observed in LPO between anaerobic trials, but the NO bioavailability increased and was higher in F + AnE than in G+AnE after exercise and recovery (p<0.05). Residual lactate was also higher under the F+AnE condition (p<0.05). During AE, there were no differences in IRH, glycemia, or NO between groups, but LPO was significantly higher after F supplementation. These results suggest that the addition of fructose to a single G supplement ingested before a glycolitic exercise can modify the glucoregulation and increases ischemic reactive hyperemia.

  17. Effects of shock 2,4-dichlorophenol (DCP) and cod loading rates on the removal of 2,4-DCP in a sequential upflow anaerobic sludge blanket/aerobic completely stirred tank reactor system.

    Science.gov (United States)

    Uluköy, A; Sponza, D T

    2008-04-01

    The treatability of 2,4-dwichlorophenol (DCP) was studied in an anaerobic/aerobic sequential reactor system. Laboratory scale upflow anaerobic sludge blanket (UASB) reactor/completely stirred tank reactors (CSTR) were operated at constant 2,4-DCP concentrations, and increasing chemical oxygen demand (COD) loading rates. The effect of shock organic loading rates on 2,4-DCP, COD removal efficiencies and methane gas production were investigated in the UASB reactor. When the organic loading rate was increased from 3.6 g l(-1) d(-1) to 30.16 g l(-1) d(-1), the COD and 2,4-DCP removal efficiencies decreased from 80 to 25% and from 99 to 60% in the UASB reactor. The optimum organic loading rates for maximum 2,4-DCP (E=99-100%) and COD (E=65-85%) removal efficiencies were 25-30 and 8-20 g-COD l(-1) d(-1), respectively. The percentage of methane of the total gas varied between 70 and 80 while the organic loadings were 18 g-COD l(-1) d(-1) and 20.36 g-COD l(-1) d(-1), respectively. During 80 days of operation, 2,4-DCP concentration was found to be below 0.5 and 0.1 mg l(-1) in aerobic reactor effluent resulting in 78 and 100% removal efficiencies. When the hydraulic retention time (HRT) was 18.72 h, the 2,4-DCP removal efficiency was 97% in the aerobic reactor. The optimum COD removal efficiency was 78.83% in anaerobic reactor effluent at an influent COD loading rate of 7.238 g-COD l(-1) d(-1) while 83.6% maximum COD removal efficiency was obtained in the aerobic reactor, resulting in a total COD removal efficiency of 96.83% in the whole system. The 2,4-DCP removal efficiency was 99% in the sequential anaerobic (UASB)/aerobic (CSTR) reactor system at COD loading rates varying between 11.46 and 30.16 g-COD l(-1) d(-1).

  18. Effects of ingesting JavaFit Energy Extreme functional coffee on aerobic and anaerobic fitness markers in recreationally-active coffee consumers

    Directory of Open Access Journals (Sweden)

    Kreider Richard B

    2007-12-01

    Full Text Available Abstract The purpose of this study was to examine the effects of ingesting JavaFit™ Energy Extreme (JEE on aerobic and anaerobic performance measures in recreationally-active male and female coffee drinkers. Five male (27.6 ± 4.2 yrs, 93.2 ± 11.7 kg, 181.6 ± 6.9 cm and five female (29 ± 4.6 yrs, 61.5 ± 9.2 kg, 167.6 ± 6.9 cm regular coffee drinkers (i.e., 223.9 ± 62.7 mg·d-1 of caffeine participated in this study. In a cross-over, randomized design, participants performed a baseline (BASELINE graded treadmill test (GXT for peak VO2 assessment and a Wingate test for peak power. Approximately 3–4 d following BASELINE testing, participants returned to the lab for the first trial and ingested 354 ml of either JEE or decaffeinated coffee (DECAF, after which they performed a GXT and Wingate test. Criterion measures during the GXT included an assessment of peakVO2 at maximal exercise, as well as VO2 at 3 minutes and 10 minutes post-exercise. Additionally, time-to-exhaustion (TTE, maximal RPE, mean heart rate (HR, mean systolic pressure (SBP, and mean diastolic blood pressure (DBP were measured during each condition. Criterion measures for the Wingate included mean HR, SBP, DBP, peak power, and time to peak power (TTP. Participants then returned to the lab approximately one week later to perform the second trial under the same conditions as the first, except consuming the remaining coffee. Data were analyzed using a one way ANOVA (p 2 at 3 minutes post-exercise when compared to BASELINE (p = 0.04 and DECAF (p = 0.02 values, which may be beneficial in enhancing post-exercise fat metabolism.

  19. 322份血培养阳性病原菌在需氧、厌氧瓶的检出率比较%Comparison of detection rates of 322 blood culture positive pathogens in aerobic and anaerobic bottles

    Institute of Scientific and Technical Information of China (English)

    陈玉莲; 徐涛; 李磊邦; 李景松; 李永赞

    2012-01-01

    OBJECTIVE To analyze the detection rates of blood culture-positive pathogens in the aerobic bottle and anaerobic bottle from Jul 2010 to Jul 2011. METHODS The culture and identification for the blood were performed with the United States BD 9050 automated blood culture system and French bio-Merieux ATBExpression automatic bacterial identification system. RESULTS Of 2700 blood culture specimens, 322 blood cultures were positive, the positive rate was 11. 9%, including gram-positive cocci (24. 5%), gram-positive bacilli (0. 6%), gram-negative cocci (0.3%), gram-negative bacilli (65.8%), anaerobic (2.0%), and fungi (6.8%); there were only 130 (40. 4%) positive blood cultures in aerobic bottle and 60 (18. 6%) positive blood cultures in anaerobic bottlei there were 132 (41. 0%} positive cultures in aerobic and anaerobic blood cultures. CONCLUSION Simultaneous culture with aerobic and anaerobic bottles for the same patient not only can fully reflect the rapidity of automatic blood culture system of rapid but also improve the positive rate of blood culture.%目的 分析2010年7月-2011年7月血培养阳性的病原菌在需氧、厌氧瓶检出率.方法 血培养及鉴定采用美国BD9050全自动血培养仪和法国生物梅里埃公司ATBExpression自动细菌鉴定仪.结果 在2700份血培养中阳性322份,阳性率为11.9%,其中革兰阳性球菌占24.5%、革兰阳性杆菌占0.6%、革兰阴性球菌占0.3%、革兰阴性杆菌占65.8%、厌氧菌占2.0%、真菌占6.8%;其中仅需氧血培养阳性130份,占40.4%,仅厌氧血培养阳性60份,占18.6%,需氧与厌氧血培养均阳性132份,占41.0%.结论 同一患者同时进行需氧瓶及厌氧瓶培养,即能充分体现自动血培养仪快速性,又能提高血培养阳性率.

  20. Biodegradation Process and Influencing Factors of Azo Using Anaerobic-aerobic Technology%偶氮染料的厌氧-好氧生物降解过程和影响因素

    Institute of Scientific and Technical Information of China (English)

    温贝贝; 刘志刚; 马鲁铭

    2013-01-01

    The total amount and kinds of azo dyes are the biggest of synthetic dyes. Usually the anaerobic-aerobic process is adopted to degrade the azo dyes, for the azo dyes can be reduced to aromatic amines in anaerobic condition, and then degraded completely in the following aerobic condition. Anaerobic reduction process of azo dyes is influenced by the structure and concentration of azo dye, the kind and concentration of substrates, other electron acceptors, redox mediator, environmental parameters such as temperature and dissolved oxygen, and hydraulic retention time. The degradation of aromatic amines under aerobic conditions is affected by its own structure, concentration, additional carbon, degradation system etc. and disturbed by the auto-oxidation of aromatic amines. Good conditions should be provided to improve the azo dyes degradation efficiency of anaerobic-aerobic technology in practical wastewater treatment.%偶氮染料是总量最大、种类最多的合成染料,其降解处理通常采用厌氧-好氧技术.厌氧过程可实现偶氮染料的还原,好氧过程则完成还原产物芳香胺的去除.厌氧过程受偶氮染料结构和浓度、底物的种类和浓度、其他电子受体、氧化还原介体、温度和DO等环境因素以及水力停留时间的影响.好氧条件下芳香胺的降解过程受其自身的结构、浓度、外加碳源以及降解体系等影响,且自氧化过程影响了芳香胺的生物降解.在实际废水处理中应创造良好的条件提高偶氮染料的厌氧-好氧生物降解效率.

  1. Intraspecific variation in aerobic and anaerobic locomotion: gilthead sea bream (Sparus aurata) and Trinidadian guppy (Poecilia reticulata) do not exhibit a trade-off between maximum sustained swimming speed and minimum cost of transport.

    Science.gov (United States)

    Svendsen, Jon C; Tirsgaard, Bjørn; Cordero, Gerardo A; Steffensen, John F

    2015-01-01

    Intraspecific variation and trade-off in aerobic and anaerobic traits remain poorly understood in aquatic locomotion. Using gilthead sea bream (Sparus aurata) and Trinidadian guppy (Poecilia reticulata), both axial swimmers, this study tested four hypotheses: (1) gait transition from steady to unsteady (i.e., burst-assisted) swimming is associated with anaerobic metabolism evidenced as excess post exercise oxygen consumption (EPOC); (2) variation in swimming performance (critical swimming speed; U crit) correlates with metabolic scope (MS) or anaerobic capacity (i.e., maximum EPOC); (3) there is a trade-off between maximum sustained swimming speed (U sus) and minimum cost of transport (COTmin); and (4) variation in U sus correlates positively with optimum swimming speed (U opt; i.e., the speed that minimizes energy expenditure per unit of distance traveled). Data collection involved swimming respirometry and video analysis. Results showed that anaerobic swimming costs (i.e., EPOC) increase linearly with the number of bursts in S. aurata, with each burst corresponding to 0.53 mg O2 kg(-1). Data are consistent with a previous study on striped surfperch (Embiotoca lateralis), a labriform swimmer, suggesting that the metabolic cost of burst swimming is similar across various types of locomotion. There was no correlation between U crit and MS or anaerobic capacity in S. aurata indicating that other factors, including morphological or biomechanical traits, influenced U crit. We found no evidence of a trade-off between U sus and COTmin. In fact, data revealed significant negative correlations between U sus and COTmin, suggesting that individuals with high U sus also exhibit low COTmin. Finally, there were positive correlations between U sus and U opt. Our study demonstrates the energetic importance of anaerobic metabolism during unsteady swimming, and provides intraspecific evidence that superior maximum sustained swimming speed is associated with superior swimming

  2. Intraspecific variation in aerobic and anaerobic locomotion: gilthead sea bream (Sparus aurata and Trinidadian guppy (Poecilia reticulata do not exhibit a trade-off between maximum sustained swimming speed and minimum cost of transport.

    Directory of Open Access Journals (Sweden)

    Jon Christian Svendsen

    2015-02-01

    Full Text Available Intraspecific variation and trade-off in aerobic and anaerobic traits remain poorly understood in aquatic locomotion. Using gilthead sea bream (Sparus aurata and Trinidadian guppy (Poecilia reticulata, both axial swimmers, this study tested four hypotheses: 1 gait transition from steady to unsteady (i.e. burst-assisted swimming is associated with anaerobic metabolism evidenced as excess post exercise oxygen consumption (EPOC; 2 variation in swimming performance (critical swimming speed; Ucrit correlates with metabolic scope (MS or anaerobic capacity (i.e. maximum EPOC; 3 there is a trade-off between maximum sustained swimming speed (Usus and minimum cost of transport (COTmin; and 4 variation in Usus correlates positively with optimum swimming speed (Uopt; i.e. the speed that minimizes energy expenditure per unit of distance travelled. Data collection involved swimming respirometry and video analysis. Results showed that anaerobic swimming costs (i.e. EPOC increase linearly with the number of bursts in S. aurata, with each burst corresponding to 0.53 mg O2 kg-1. Data are consistent with a previous study on striped surfperch (Embiotoca lateralis, a labriform swimmer, suggesting that the metabolic cost of burst swimming is similar across various types of locomotion. There was no correlation between Ucrit and MS or anaerobic capacity in S. aurata indicating that other factors, including morphological or biomechanical traits, influenced Ucrit. We found no evidence of a trade-off between Usus and COTmin. In fact, data revealed significant negative correlations between Usus and COTmin, suggesting that individuals with high Usus also exhibit low COTmin. Finally, there were positive correlations between Usus and Uopt. Our study demonstrates the energetic importance of anaerobic metabolism during unsteady swimming, and provides intraspecific evidence that superior maximum sustained swimming speed is associated with superior swimming economy and optimum

  3. Mechanical-biological waste treatment and anaerobic processes. 59. information meeting, Neuwied, October 1999; Mechanisch-biologische Restabfallbehandlung und Anaerobverfahren. 59. Informationsgespraech in Neuwied im Oktober 1999

    Energy Technology Data Exchange (ETDEWEB)

    Hangen, H.O.; Euler, H.; Leonhardt, H.W. [comps.

    1999-10-01

    This proceedings volume discusses the specifications for and cost of mechanical-biological waste treatment, the optimisation of economic efficiency and pollutant emissons, the combination of mechanical-biological and thermal waste treatment processes, the value of mechanical-biological waste treatment, waste management concepts, process engineering and practical experience, and the eco-balance of the process. [German] Themen dieses Proceedingsbandes sind: Anforderungen und Kosten der mechanisch-biologischen Abfallbehandlung; Optimierung der Wirtschaftlichkeit und Emissionssituation; Kombination von mechanisch-biologischer und thermischer Muellbehandlung; Bewertung der mechanisch-biologischen Abfallbehandlung, Abfallwirtschaftskonzepte, Verfahrenstechnik und Betriebserfahrungen; Oekobilanz. (SR)

  4. Successful treatment of high azo dye concentration wastewater using combined anaerobic/aerobic granular activated carbon-sequencing batch biofilm reactor (GAC-SBBR): simultaneous adsorption and biodegradation processes.

    Science.gov (United States)

    Hosseini Koupaie, E; Alavi Moghaddam, M R; Hashemi, S H

    2013-01-01

    The application of a granular activated carbon-sequencing batch biofilm reactor (GAC-SBBR) for treatment of wastewater containing 1,000 mg/L Acid Red 18 (AR18) was investigated in this research. The treatment system consisted of a sequencing batch reactor equipped with moving GAC as biofilm support. Each treatment cycle consisted of two successive anaerobic (14 h) and aerobic (8 h) reaction phases. Removal of more than 91% chemical oxygen demand (COD) and 97% AR18 was achieved in this study. Investigation of dye decolorization kinetics showed that the dye removal was stimulated by the adsorption capacity of the GAC at the beginning of the anaerobic phase and then progressed following a first-order reaction. Based on COD analysis results, at least 77.8% of the dye total metabolites were mineralized during the applied treatment system. High-performance liquid chromatography analysis revealed that more than 97% of 1-naphthyalamine-4-sulfonate as one of the main sulfonated aromatic constituents of AR18 was removed during the aerobic reaction phase. According to the scanning electron microscopic analysis, the microbial biofilms grew in most cavities and pores of the GAC, but not on the external surfaces of the GAC.

  5. 厌氧一好氧交替工艺生物除磷及活性污泥特殊染色%Biological Removal of Phophous Using Aerobic/Anaerobic Alternation Process and Peculiar Staining of Actived Sludge

    Institute of Scientific and Technical Information of China (English)

    田淑嫒; 李征; 王景峰

    2001-01-01

    研究了“厌氧一好氧交替工艺”生物除磷工艺,确定最佳厌氧时间为1.5 h,最佳好氧时间为4 h。应 用特殊染色法直接将活性污泥制成切片染色,通过显微镜镜检PHB、Poly-p(聚磷颗粒),可以监测除磷效果。%biological removal of phophous by “aerobic/anaerobic alternation process” was tested, with the optimum aerobic time determined to be 1.5 hours and the optimum anaerobic time to be 4 hours. The result of phosphorus removal can be monitored using peculiar staining process, with which activated sludge is directly made into a specimen on a glass slide and stained, and through detectionof PHB and poly-p by microscope.

  6. Evaluation of integrated anaerobic/aerobic fixed-bed sequencing batch biofilm reactor for decolorization and biodegradation of azo dye acid red 18: comparison of using two types of packing media.

    Science.gov (United States)

    Hosseini Koupaie, E; Alavi Moghaddam, M R; Hashemi, S H

    2013-01-01

    Two integrated anaerobic/aerobic fixed-bed sequencing batch biofilm reactor (FB-SBBR) were operated to evaluate decolorization and biodegradation of azo dye Acid Red 18 (AR18). Volcanic pumice stones and a type of plastic media made of polyethylene were used as packing media in FB-SBBR1 and FB-SBBR2, respectively. Decolorization of AR18 in both reactors followed first-order kinetic with respect to dye concentration. More than 63.7% and 71.3% of anaerobically formed 1-naphthylamine-4-sulfonate (1N-4S), as one of the main sulfonated aromatic constituents of AR18 was removed during the aerobic reaction phase in FB-SBBR1 and FB-SBBR2, respectively. Based on statistical analysis, performance of FB-SBBR2 in terms of COD removal as well as biodegradation of 1N-4S was significantly higher than that of FB-SBBR1. Spherical and rod shaped bacteria were the dominant species of bacteria in the biofilm grown on the pumice stones surfaces, while, the biofilm grown on surfaces of the polyethylene media had a fluffy structure.

  7. Anaerobic bacteria in otitis media.

    Science.gov (United States)

    Fulghum, R S; Daniel, H J; Yarborough, J G

    1977-01-01

    Anaerobic bacteria, Peptostrepotococcus intermedius and Propionibacterium acnes, were found in mixed culture specimens from four to ten tested cases of chronic secretory otitis media. These anaerobic bacteria were in a mixed infection flora with aerobic bacteria most often Staphylococcus epidermidis and Cornybacterium sp. which do not fit any established species. The findings of anaerobic bacteria in otitis media is consistent with the sporadic report of the involvement of anaerobic bacteria in otitis media in the literature since 1898.

  8. Comparative in vitro activities of SMT19969, a new antimicrobial agent, against Clostridium difficile and 350 gram-positive and gram-negative aerobic and anaerobic intestinal flora isolates.

    Science.gov (United States)

    Goldstein, Ellie J C; Citron, Diane M; Tyrrell, Kerin L; Merriam, C Vreni

    2013-10-01

    The comparative in vitro activity of SMT19969, a novel, narrow-spectrum, nonabsorbable agent, was studied against 50 ribotype-defined Clostridium difficile strains, 174 Gram-positive and 136 Gram-negative intestinal anaerobes, and 40 Gram-positive aerobes. SMT19969 was one dilution more active against C. difficile isolates (MIC range, 0.125 to 0.5 μg/ml; MIC90, 0.25 μg/ml), including ribotype 027 strains, than fidaxomicin (range, 0.06 to 1 μg/ml; MIC90, 0.5 μg/ml) and two to six dilutions lower than either vancomycin or metronidazole. SMT19969 and fidaxomicin were generally less active against Gram-negative anaerobes, especially the Bacteroides fragilis group species, than vancomycin and metronidazole, suggesting that SMT19969 has a lesser impact on the normal intestinal microbiota that maintain colonization resistance. SMT19969 showed limited activity against other Gram-positive anaerobes, including Bifidobacteria species, Eggerthella lenta, Finegoldia magna, and Peptostreptococcus anaerobius, with MIC90s of >512, >512, 64, and 64 μg/ml, respectively. Clostridium species showed various levels of susceptibility, with C. innocuum being susceptible (MIC90, 1 μg/ml) and C. ramosum and C. perfringens being nonsusceptible (MIC90, >512 μg/ml). Activity against Lactobacillus spp. (range, 0.06 to >512 μg/ml; MIC90, >512 μg/ml) was comparable to that of fidaxomicin and varied by species and strain. Gram-positive aerobic cocci (Staphylococcus aureus, Enterococcus faecalis, E. faecium, and streptococci) showed high SMT19969 MIC90 values (128 to >512 μg/ml).

  9. A study on two phase anaerobic-aerobic treatment of white wine wastewater%两相厌氧—好氧技术处理白酒淘洗废水试验研究

    Institute of Scientific and Technical Information of China (English)

    张学洪; 陈志强; 吕炳南; 陈文兵; 王敦球; 李金城

    2001-01-01

    Two phase anaerobic-aerobic process is investigated for the treatment of white wine wastewater. The anaerobic contact pond of this process is packed with plastic ring, then a SBR pond anaerobic baffle reactor follows. The present study focuses on the design feature and operating results of wastewater treatment. The operating results show that the treatment effect is fair and stable. The inlet CODCr is around 3 000 mg/L,and that for the effluent is lower than 100 mg/L,and the problem in operation is summarized.%采用两相厌氧—好氧工艺,在厌氧接触池投入塑料环,后接自行研究开发的厌氧挡板反应器(内设20 μm沸石载体),再接SBR反应池处理白酒淘洗生产废水。重点介绍了废水处理工程的设计特点和运行效果。运行结果表明了处理效果十分稳定,进水CODCr在3 000 mg/L左右,出水均在100 mg/L以下,并对调试运行中出现的问题进行了讨论。

  10. Anaerobic Biodegradation of Detergent Surfactants

    OpenAIRE

    Erich Jelen; Ute Merrettig-Bruns

    2009-01-01

    Detergent surfactantscan be found in wastewater in relevant concentrations. Most of them are known as ready degradable under aerobic conditions, as required by European legislation. Far fewer surfactants have been tested so far for biodegradability under anaerobic conditions. The natural environment is predominantly aerobic, but there are some environmental compartments such as river sediments, sub-surface soil layer and anaerobic sludge digesters of wastewater treatment plants which have str...

  11. Reator compartimentado anaeróbio/aeróbio: sistema de baixo custo para tratamento de esgotos de pequenas comunidades Anaerobic/aerobic baffled reactor: low cost system for sewage treatment in small communities

    Directory of Open Access Journals (Sweden)

    Gustavo H. R. da Silva

    2005-06-01

    Full Text Available No Brasil são conhecidas e usadas várias técnicas de tratamento de esgoto, desde os mais sofisticados sistemas de tratamento aos mais simples processos. Uma alternativa economicamente viável aos sistemas de tratamento de esgotos sanitários é o processo anaeróbio, o qual tem recebido especial atenção nas pesquisas desenvolvidas no País, notadamente os reatores anaeróbios de alta taxa, unidades compactas e com boa eficiência de tratamento. Dentre as alternativas tecnológicas capazes de proporcionar as referidas variações das condições ambientais necessárias à ocorrência dos processos, está o uso de reatores compartimentados anaeróbio/aeróbio. Este trabalho apresenta, como alternativa, um reator compartimentado anaeróbio/aeróbio, tratando esgoto sanitário, como possibilidade de ser utilizado em pequenas comunidades urbanas e rurais. O desempenho do sistema foi avaliado em diversos tempos de detenção hidráulica (TDH, nos quais os melhores valores de Demanda Química de Oxigênio Total (DQOtotal - 73,7% e Sólidos em Suspensão Totais (SST - 78,8%, foram obtidos com TDHtotal de 8 horas (4 horas para a fase anaeróbia e 4 horas para a fase aeróbia. O reator compartimentado apresentou a característica de estabilidade operacional necessária aos sistemas de baixo custo.Several techniques of sewage treatment, from the most sophisticated to the simplest processes, are known in Brazil. An economically viable alternative for the systems of treatment of sanitary sewage is the anaerobic process, which has been given special attention in Brazilian research programs, mainly in the field of high rate anaerobic reactors as they are compact units and have high efficiency. The use of baffled anaerobic/aerobic reactors is one technological solution to provide the necessary variations in environmental conditions to foster the processes mentioned. In this paper an alternative is presented in the form of a baffled anaerobic/aerobic

  12. Degradation of organic pollutants in sewage sludge by aerobic-thermophilic sludge treatment. Final report; Abbau organischer Schadstoffe im Klaerschlamm durch aerob-thermophile Schlammbehandlung. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Prechtl, S.

    1999-07-01

    A process for reduction of organic polllutants in sewage sludge was to be developed and optimized. The organic fraction of the solid matter in sewage sludge containes more than 300 different pollutant. Apart from the substances classified in the Sewage Sludge Ordinance (dioxins/furans, PCB), there are others that have been considered relevant as well but for which no sufficient data base is available. The research project investigated whether aerobic-thermophilic treatment (AT) would improve the sewage sludge quality with regard to phthalates (di(2-ethylhexyl)phthalate, DEHP), PAH and 4-nonylphenol (4-NP, a degradation product of nonionic tensides). Pollutants were analzyed by HPLC and GC/MS. The concentration of DEHP and 4-NP was reduced by 70% resp. 50% in laboratory experiments with doped sludges and by 61% resp. 53% in undoped sludges. In semi-industrial tests, a 14% reduction was achieved for DEHP and a 68% reduction for 4-NP. In the case of pyrene, the degradation was up to 57% in the laboratory experiments and 22% in semi-industrial tests. A combined process of short-term anaerobic digestion and AT resulted in a 60% reduction in the case of DEHP. Up to anthracene, PAH were reduced as well. In the case of higher-condensed PAH (basic load in the ppb range) there was no clear result. In the case of 4-NP, the degradation effect was counterbalanced by the formation of new 4-NP from alkylphenol ethoxylates in both anaerobic and aerobic conditions. The results prove the correlation between sewage sludge hygienisation and the time of residue in the reactor system. No enterobacteriaceae were found after a treatment of 96 h, both in the semi-industrial and the laboratory reactors. In activated sludge, a phenol-degrading mixed bacteria culture could be isolated which was also capable of degrading 4-NP in thermophilic conditions. [German] Das Ziel des Forschungsvorhabens war die Entwicklung/Optimierung eines Verfahrens zur Reduktion organischer Schadstoffe im

  13. Gasto energético aeróbico y anaeróbico en un circuito con cargas a seis intensidades diferentes. (Aerobic and anaerobic energy expenditure during at circuit weight training through six different intensities.

    Directory of Open Access Journals (Sweden)

    Rocío Cupeiro Coto

    2011-07-01

    Full Text Available ResumenEl entrenamiento con cargas es una actividad anaeróbica glucolítica intensa y se ha comprobado que el error en las estimaciones del gasto energético en esta actividad varía entre un 13 y un 30%. El principal objetivo de este trabajo es describir la contribución anaeróbica de energía en un circuito con cargas. Doce hombres (20-26 años y diecisiete mujeres (18-29 años estudiantes de Ciencias de la Actividad Física y del Deporte realizaron un entrenamiento en circuito de cargas a 6 intensidades diferentes (entre el 30% y 80% de su 15RM. Durante la totalidad de los circuitos se registró el gasto energético aeróbico por calorimetría indirecta, la frecuencia cardiaca con pulsómetro Polar® y la concentración de lactato en sangre capilar para medir la contribución anaeróbica. El incremento que produjo la energía anaeróbica se situó entre el 5,1% y un máximo del 13,5%, lo que hace evidente que medir o no la contribución anaeróbica en el entrenamiento en circuito puede provocar un error medio del 9,65%. Existen diferencias significativas (PAbstractResistance training is an intense anaerobic glycolytic activity and has been shown that estimates of energy expenditure in this activity turn out into an error that varies between 13 and 30%. The main aim of this paper is to describe the anaerobic energy contribution in circuit weight training. Twelve men (20-26 years and seventeen women (18-29 years students in Science of Physical Activity and Sport performed circuit training at six different intensities (between 30% and 80% of 15RM. During all the circuits aerobic energy expenditure was registered by indirect calorimetry, heart rate with Polar® monitors and lactate concentration in capillary blood to measure the anaerobic contribution. The increased due to anaerobic energy was between 5,1% and a maximum of 13,5%, which clearly means that to measure or not the anaerobic contribution in circuit training can lead to an average

  14. Mimicking the oxygen minimum zones: stimulating interaction of aerobic archaeal and anaerobic bacterial ammonia oxidizers in a laboratory-scale model system

    NARCIS (Netherlands)

    Yan, J.; Haaijer, S.C.M.; Op den Camp, H.J.M.; van Niftrik, L.; Stahl, D.A.; Könneke, M.; Rush, D.; Sinninghe Damsté, J.S.; Hu, Y.Y.; Jetten, M.S.M.

    2012-01-01

    In marine oxygen minimum zones (OMZs), ammonia-oxidizing archaea (AOA) rather than marine ammonia-oxidizing bacteria (AOB) may provide nitrite to anaerobic ammonium-oxidizing (anammox) bacteria. Here we demonstrate the cooperation between marine anammox bacteria and nitrifiers in a laboratory-scale

  15. Evaluation of the detoxication efficiencies for acrylonitrile wastewater treated by a combined anaerobic oxic-aerobic biological fluidized tank (A/O-ABFT) process: Acute toxicity and zebrafish embryo toxicity.

    Science.gov (United States)

    Na, Chunhong; Zhang, Ying; Deng, Minjie; Quan, Xie; Chen, Shuo; Zhang, Yaobin

    2016-07-01

    Acrylonitrile (ACN) wastewater generated during ACN production has been reported to be toxic to many aquatic organisms. However, few studies have evaluated toxicity removal of ACN wastewater during and after the treatment process. In this study, the detoxication ability of an ACN wastewater treatment plant (WWTP) was evaluated using Daphnia magna, Danio rerio and zebrafish embryo. This ACN WWTP has a combined anaerobic oxic-aerobic biological fluidized tank (A/O-ABFT) process upgraded from the traditional anaerobic oxic (A/O) process. Moreover, the potential toxicants of the ACN wastewaters were identified by gas chromatography-mass spectrometry (GC-MS). The raw ACN wastewater showed high acute and embryo toxicity. 3-Cyanopyridine, succinonitrile and a series of nitriles were detected as the toxic contributors of ACN wastewater. The A/O process was effective for the acute and embryo toxicity removal, as well as the organic toxicants. However, the A/O effluent still showed acute and embryo toxicity which was attributed by the undegraded and the newly generated toxicants during the A/O process. The residual acute and embryo toxicity as well as the organic toxicants in the A/O effluent were further reduced after going through the downstream ABFT process system. The final effluent displayed no significant acute and embryo toxicity, and less organic toxicants were detected in the final effluent. The upgrade of this ACN WWTP results in the improved removal efficiencies for acute and embryo toxicity, as well as the organic toxicants.

  16. The effect of tannic compounds on anaerobic wastewater treatment.

    NARCIS (Netherlands)

    Field, J.A.

    1989-01-01

    Anaerobic wastewater treatment is an alternative to the conventional aerobic treatment processes for the removal of easily biodegradable organic matter in medium to high strength industrial wastestreams. Anaerobic treatment has several advantages, however one important disadvantage is the high sensi

  17. Application of Box-Wilson experimental design method for 2,4-dinitrotoluene treatment in a sequential anaerobic migrating blanket reactor (AMBR)/aerobic completely stirred tank reactor (CSTR) system.

    Science.gov (United States)

    Kuşçu, Özlem Selçuk; Sponza, Delia Teresa

    2011-03-15

    A sequential aerobic completely stirred tank reactor (CSTR) following the anaerobic migrating blanket reactor (AMBR) was used to treat a synthetic wastewater containing 2,4-dinitrotoluene (2,4-DNT). A Box-Wilson statistical experiment design was used to determine the effects of 2,4-DNT and the hydraulic retention times (HRTs) on 2,4-DNT and COD removal efficiencies in the AMBR reactor. The 2,4-DNT concentrations in the feed (0-280 mg/L) and the HRT (0.5-10 days) were considered as the independent variables while the 2,4-DNT and chemical oxygen demand (COD) removal efficiencies, total and methane gas productions, methane gas percentage, pH, total volatile fatty acid (TVFA) and total volatile fatty acid/bicarbonate alkalinity (TVFA/Bic.Alk.) ratio were considered as the objective functions in the Box-Wilson statistical experiment design in the AMBR. The predicted data for the parameters given above were determined from the response functions by regression analysis of the experimental data and exhibited excellent agreement with the experimental results. The optimum HRT which gave the maximum COD (97.00%) and 2,4-DNT removal (99.90%) efficiencies was between 5 and 10 days at influent 2,4-DNT concentrations 1-280 mg/L in the AMBR. The aerobic CSTR was used for removals of residual COD remaining from the AMBR, and for metabolites of 2,4-DNT. The maximum COD removal efficiency was 99% at an HRT of 1.89 days at a 2,4-DNT concentration of 239 mg/L in the aerobic CSTR. It was found that 280 mg/L 2,4-DNT transformed to 2,4-diaminotoluene (2,4-DAT) via 2-amino-4-nitrotoluene (2-A-4-NT) and 4-amino-2-nitrotoluene (4-A-2-NT) in the AMBR. The maximum 2,4-DAT removal was 82% at an HRT of 8.61 days in the aerobic CSTR. The maximum total COD and 2,4-DNT removal efficiencies were 99.00% and 99.99%, respectively, at an influent 2,4-DNT concentration of 239 mg/L and at 1.89 days of HRT in the sequential AMBR/CSTR.

  18. Study on Characteristics of Aerobic and Anaerobic Capabilities of Elite Male Handball Players%优秀男子手球运动员有氧、无氧能力特征研究

    Institute of Scientific and Technical Information of China (English)

    张莉; 林丽雅

    2016-01-01

    目的 :通过对不同位置手球运动员有氧、无氧能力进行测试和分析 ,探讨优秀男子手球运动员有氧、无氧能力特征 ,为运动员个体化身体机能评定 ,训练效果评价及个体化训练安排提供依据.方法 :36名优秀男子手球运动员分别完成最大吸氧量(.VO2 max )测试、Win-gate 30 s无氧功测试和不同距离跑动能力测试 ,测试过程中记录运动员最大吸氧量等有氧能力相关指标 ,最大功率、乳酸等无氧能力相关指标及不同距离跑测试成绩.结果 :1)广东男子手球运动员最大吸氧量相对值接近世界级男子手球运动员水平 ;2)对于不同位置手球运动员而言 ,最大吸氧量水平最高的是內锋运动员 ,最低的是中卫运动员 ,两者有显著性差异(P<0 .05);3)广东运动员的最大功率绝对值、平均功率和疲劳指数均明显差于国家队(P<0 .01);4)我国运动员的最大功率绝对值、最大功率相对值、乳酸值均低于世界级运动员 ;5)內锋运动员的最大功率、平均功率和乳酸显著高于边锋(P< 0 .05);6)在不同距离跑动能力方面 ,不同位置运动员的能力有所不同.结论 :1)手球运动员的有氧能力与足球、曲棍球运动员水平相近 ,低于篮球运动员 ;2)我国运动员的有氧能力接近世界级水平 ;3)內锋运动员的有氧能力显著高于中卫 ;4)我国手球运动员的爆发力低于足球、曲棍球项目运动员的爆发力 ,速度耐力能力与另两项目运动员水平接近 ;5)我国手球运动员的无氧能力与世界级运动员相比存在较大差距 ;6)內锋运动员的无氧能力显著高于边锋.%Objective :The purpose of this study was to determine the aerobic and anaerobic capa-bilities of elite handball players through analyzing and testing of different location of the hand-ball players' aerobic and anaerobic capacity ,and provide the basis for individual function evalu-ation ,training effect evaluation

  19. Waste decomposition and off-gas emissions of dry, single-stage anaerobic digestion processes followed by a composting stage: The example of the KOMPOGAS process; Abbauleistung und Abluftemissionen bei trockenen, einstufigen Vergaerungsverfahren mit nachgeschalteter Rotte am Beispiel des KOMPOGAS-Verfahrens

    Energy Technology Data Exchange (ETDEWEB)

    Fricke, K. [Technische Univ. Braunschweig (Germany). Leichtweiss-Institut fuer Wasserbau; Leisner, R. [KOGAS AG, Uzwil (Switzerland); Wallmann, R. [Ingenieurgemeinschaft Witzenhausen, Fricke und Turk GmbH, Witzenhausen (Germany)

    2001-07-01

    The working group IGW/LWI (Ingenieurgemeinschaft Witzenhausen Fricke and Turk GmbH and Leichtweiss Institute of TU Brunswick University) carried out scientific and technical investigations of anaerobic digestion and subsequent composting at the mechanical-biological waste treatment plant at Kufstein/Tyrol on behalf of KOGAS GmbH. The investigations started in May 2000; they were to provide information for analysis and optimisation of the process and products with a view to the minimisation of residues for dumping or incineration. The following subjects were investigated: Mass balance; decomposition rate; gas yields and gas quality; quality of residues for dumping (biological stability and calorific value); off-gas emissions; process control. Performance data of the plant will provide a basis for further planning and will be interpreted against the background of current legal boundary conditions. [German] Die Arbeitsgemeinschaft IGW/LWI (Ingenieurgemeinschaft Witzenhausen Fricke and Turk GmbH und Leichtweiss-Institut der TU Braunschweig) wurde im Mai 2000 von der KOGAS GmbH beauftragt, die wissenschaftlich-technische Begleitung eines Versuches zur Vergaerung und Nachrotte von Restmuell auf dem Gelaende der MBA Kufstein/Tirol durchzufuehren. Ziel der Untersuchung war die Ueberpruefung und Optimierung des Vergaerungs- und Nachrotteprozesses sowie der Materialaufbereitung und Konfektionierung im Hinblick auf die Minimierung von Stofffluessen in die energetische Verwertung bzw. thermische Behandlung. Im Rahmen des Vorhabens wurden folgende Thermokomplexe bearbeitet: - Massenbilanz, - Abbauleistung, - Gasertrag und Gasqualitaet, - Qualitaet der zu deponierenden Abfaelle (biologische Stabilitaet und Heizwert), - Abluftemissionen, - Prozesssteuerung. Leistungsdaten der Verfahren sollten als Planungsgrundlagen bereitgestellt und vor dem Hintergrund der aktuellen rechtlichen Rahmenbedingungen bewertet werden. (orig.)

  20. RISK FACTORS IN NEONATAL ANAEROBIC INFECTIONS

    Directory of Open Access Journals (Sweden)

    M. S. Tabib

    2008-06-01

    Full Text Available Anaerobic bacteria are well known causes of sepsis in adults but there are few studies regarding their role in neonatal sepsis. In an attempt to define the incidence of neonatal anaerobic infections a prospective study was performed during one year period. A total number of 400 neonates under sepsis study were entered this investigation. Anaerobic as well as aerobic cultures were sent. The patients were subjected to comparison in two groups: anaerobic culture positive and anaerobic culture negative and this comparison were analyzed statistically. There were 7 neonates with positive anaerobic culture and 35 neonates with positive aerobic culture. A significant statistical relationship was found between anaerobic infections and abdominal distention and pneumonia. It is recommended for those neonates with abdominal distention and pneumonia refractory to antibiotic treatment to be started on antibiotics with anaerobic coverage.

  1. Field-based and laboratory stable isotope probing surveys of the identities of both aerobic and anaerobic benzene-metabolizing microorganisms in freshwater sediment

    Energy Technology Data Exchange (ETDEWEB)

    Liou, J.S.C.; DeRito, C.M.; Madsen, E.L. [Cornell University, Ithaca, NY (United States). Dept. of Microbiology

    2008-08-15

    Laboratory incubations of coal-tar waste-contaminated sediment microbial communities under relatively controlled physiological conditions were used to interpret results of a field-based stable isotope probing (SIP) assay. Biodegradation activity of {sub 13}C-benzene was examined by GC/MS determination of net (CO{sub 2})-{sub 13}C production and by GC headspace analysis of benzene loss. In anaerobic laboratory incubations of benzene at 10 p.p.m., greater than 60% of the substrate was eliminated within 15 days. During anaerobic incubations of 200 p.p.m. benzene (70 days), 0.9% benzene mineralization occurred. When benzene (36 p.p.m.) was added to sediment with air in the serum-bottle headspace, 14% of the initial C-13 was mineralized to (CO{sub 2})-{sub 13}C in 2.5 days. In the field experiment (178 {mu} g {sub 13}C-benzene dosed to undisturbed sediments), net (CO{sub 2})-{sub 13}C production reached 0.3% within 8.5 h. After isopycnic separation of {sub 13}C (heavy)-labelled DNA from the above biodegradation assays, sequencing of {sub 13}C-DNA clone libraries revealed a broad diversity of taxa involved in benzene metabolism and distinctive libraries for each biodegradation treatment. Perhaps most importantly, in the field SIP experiment the clone libraries produced were dominated by Pelomonas (betaproteobacteria) sequences similar to those found in the anaerobic 10 p.p.m. benzene laboratory experiment. These data indicate that the physiological conditions that prevail and govern in situ biodegradation of pollutants in the field may be interpreted by knowing the physiological preferences of potentially active populations.

  2. 不同结构好氧/厌氧潜流人工湿地微生物群落代谢特性%Characteristics of microbial community metabolism in aerobic/anaerobic subsurface flow constructed wetland

    Institute of Scientific and Technical Information of China (English)

    赵艳; 李锋民; 王昊云; 李扬; 王震宇

    2012-01-01

    Four different structures of aerobic/anaerobic subsurface flow constructed wetland had different purification efficiencies for COD and N.To investigate the characteristics of the microbial community metabolism and its relationship with water purification,carbon source analysis,principal component analysis and cluster analysis were used on the average well color development(AWCD) values obtained from the Biolog Ecoplate.The results showed that the degree of microbial utilization of carbohydrate and its derivatives,amino acids and its derivatives were significantly higher than that of fatty acid,lipid,metabolic intermediate and secondary metabolites.The degree of microbial utilization of four kinds of carbon sources in the sampling site(the sampling site which proceeded anaerobic reaction) in the group of OBAAO(aerobic-buffer-anaerobic anoxic-aerobic) aeration were the lowest,and it had significant difference with that of the other sampling sites(p0.05).The results of principal component analysis and cluster analysis indicated that the microbial community of the sampling site 3.3 in the group of OBAAO aeration had high difference from those of the other 3 sampling sites which were preceding anaerobic reaction.The group of OBAAO aeration extending the length of anaerobic stage provided the anaerobic environment for microorganisms.However,because of the lack of carbon source as energy,the microbial activity was inhibited.The microbial utilization degrees in sampling site 4.3(the sampling site which proceeded anaerobic reaction) in the group of OBAAO aeration multiple spot influent had certain advantage.The group of OBAAO aeration multiple spot influent,by adding influent and extending the length of anaerobic stage,effectively promoted the strength of denitrification,and increased the removal efficiency of nitrogen within horizontal subsurface flow constructed wetlands.%4种不同结构的好氧/厌氧多级串联潜流人工湿地对COD和氮的去

  3. Discovery of competing anaerobic and aerobic pathways in umpolung amide synthesis allows for site-selective amide 18O-labeling.

    Science.gov (United States)

    Shackleford, Jessica P; Shen, Bo; Johnston, Jeffrey N

    2012-01-03

    The mechanism of umpolung amide synthesis was probed by interrogating potential sources for the oxygen of the product amide carbonyl that emanates from the α-bromo nitroalkane substrate. Using a series of (18)O-labeled substrates and reagents, evidence is gathered to advance two pathways from the putative tetrahedral intermediate. Under anaerobic conditions, a nitro-nitrite isomerization delivers the amide oxygen from nitro oxygen. The same homolytic nitro-carbon fragmentation can be diverted by capture of the carbon radical intermediate with oxygen gas (O(2)) to deliver the amide oxygen from O(2). This understanding was used to develop a straightforward protocol for the preparation of (18)O-labeled amides in peptides by simply performing the umpolung amide synthesis reaction under an atmosphere of 18O2.

  4. Photocatalytic transformations of CCl{sub 3}Br, CBr{sub 3}F, CHCl{sub 2}Br and CH{sub 2}BrCl in aerobic and anaerobic conditions

    Energy Technology Data Exchange (ETDEWEB)

    Calza, P.; Minero, C.; Pelizzetti, E. [Dipartimento di Chimica Analitica, Universita di Torino, 10125 Torino (Italy); Hiskia, A.; Papaconstantinou, E. [Institute of Physical Chemistry, NCSR Demokritos, 15310 Athens (Greece)

    2001-01-01

    Phototransformations of halomethanes containing chlorine and bromine (CCl{sub 3}Br, CHCl{sub 2}Br, CH{sub 2}ClBr) or bromine and fluorine (CBr{sub 3}F) have been investigated under aerobic and anaerobic conditions both in homogeneous system and heterogeneous photocatalysis. For all of those compounds, the complete disappearance of the primary compound and the stoichiometric concentration of halides was achieved. Several halogenated intermediates and oxygenated compounds were identified, so that it was possible to predict the degradation pathways followed by such halomethanes. Whereas the reductive steps are predominant in the initial degradation of CCl{sub 3}Br and CBr{sub 3}F, the oxidative steps are predominant in the initial CH{sub 2}ClBr steps. The two pathways have comparable importance for CHCl{sub 2}Br degradation. Methanol, acting as a hole scavenger, strongly increases the rate of disappearance for CCl{sub 3}Br and CBr{sub 3}F.

  5. 石灰预处理/厌氧水解/好氧工艺处理甲醛废水%Lime Pretreatment/Anaerobic Hydrolysis/Aerobic Process for Treatment of Formaldehyde Wastewater

    Institute of Scientific and Technical Information of China (English)

    卢毅明; 刘霞; 贾志宇

    2011-01-01

    结合某化工厂甲醛废水处理工程的实际运行情况,介绍了石灰预处理/厌氧水解/好氧处理工艺流程、主要构筑物及设计参数.运行结果表明,该工艺对COD、甲醛的平均去除率分别达到98.4%和99.9%,出水各项指标均达到上海市(DB 31/199-1997)的二级标准.系统运行稳定,耐冲击能力强,运行成本低.%Combined with the operation of formaldehyde wastewater treatment project in a chemical plant, the flow and principle of lime pretreatment/anaerobic hydrolysis/aerobic process are introduced.The actual operation results show that the removal rates of COD and formaldehyde are more than 98.4%and 99.9% respectively.The various effluent indexes achieve the requirements of Integrated Wastewater Discharge Standard (DB 31/199 -1997) of Shanghai City.The system operates stably and has stronger resistance to shock loading and low operation cost.

  6. Optimization for low C/N sewage treatment in an anaerobic/aerobic simultaneous nitrification-endogenous denitrification and phosphorous removal system%厌氧/好氧SNEDPR系统处理低C/N污水的优化运行

    Institute of Scientific and Technical Information of China (English)

    王晓霞; 王淑莹; 赵骥; 戴娴; 彭永臻

    2016-01-01

    This study focused on the nitrogen (N) and phosphorus (P) removal performance optimization in simultaneous nitrification-endogenous denitrification and phosphorus removal (SNEDPR) systems. An anaerobic (180min)/aerobic sequencing batch reactor (SBR) fed with domestic wastewater was studied for optimization of N and P removal performance of SNEDPR by regulating the aerobic dissolved oxygen (DO) concentration (0.3~1.0mg/L) and aerobic duration time (150~240min). FISH technology was also employed to analyze the population dynamics of functional microorganisms in the SNEDPR system. Results indicated that the effluent PO43--P concentration was below 0.4mg/L, effluent TN concentration decreased from 14.3mg/L to 8.7mg/L, and TN removal efficiency increased from 75% to 84% with aerobic DO concentration decreased from 1.0mg/L to 0.3mg/L and aerobic duration time increased from 150min to 240min. SNED was enhanced by the decreased aerobic DO concentration, with SNED efficiency increased from 34.7% to 63.8%. The enhanced SNED reduced the effluent NO3--N concentration, improved the N removal performance, and strengthened the intracellular carbon storage at the following anaerobic stage. FISH results showed that the populations of PAOs, GAO and AOB (ammonia oxidizing bacteria) still maintained at high levels in the 127-day optimized SNEDPR-SBR (accounting for 29%±3%, 20%±3% and 13%±3% of total biomass, respectively), which ensured the P uptake, nitrification and denitrification; however, NOB (nitrite oxidizing bacteria) reduced by 50%, which provided a possibility to achieve N removal through simultaneous partial nitrification-endogenous denitrification in the SNEDPR.%为实现同步硝化内源反硝化除磷(SNEDPR)系统的优化运行,以实际生活污水为处理对象,采用厌氧(180min)/好氧运行的SBR反应器,并通过联合调控好氧段溶解氧(DO)浓度(0.3~1.0mg/L)和好氧时间(150~240min),考察了该系统脱氮除磷特性.并结合荧光原位杂

  7. Occurrence and removal of six pharmaceuticals and personal care products in a wastewater treatment plant employing anaerobic/anoxic/aerobic and UV processes in Shanghai, China.

    Science.gov (United States)

    Wang, Dan; Sui, Qian; Lu, Shu-Guang; Zhao, Wen-Tao; Qiu, Zhao-Fu; Miao, Zhou-Wei; Yu, Gang

    2014-03-01

    The occurrence and removal of six pharmaceuticals and personal care products (PPCPs) including caffeine (CF), N, N-diethyl-meta-toluamide (DEET), carbamazepine, metoprolol, trimethoprim (TMP), and sulpiride in a municipal wastewater treatment plant (WWTP) in Shanghai, China were studied in January 2013; besides, grab samples of the influent were also taken every 6 h, to investigate the daily fluctuation of the wastewater influent. The results showed the concentrations of the investigated PPCPs ranged from 17 to 11,400 ng/L in the WWTP. A low variability of the PPCP concentrations in the wastewater influent throughout the day was observed, with the relative standard deviations less than 25 % for most samples. However, for TMP and CF, the slight daily fluctuation still reflected their consumption patterns. All the target compounds except CF and DEET, exhibited poor removal efficiencies (treatment process, probably due to the low temperature in the bioreactor, which was unfavorable for activated sludge. While for the two biodegradable PPCPs, CF, and DEET, the anaerobic and oxic tank made contributions to their removal while the anoxic tank had a negative effect to their elimination. The tertiary UV treatment removed the investigated PPCPs by 5-38 %, representing a crucial polishing step to compensate for the poor removal by the biologic treatment process in winter.

  8. Influence of an aerobic fungus grown on solid culture on ruminal degradability and on a mixture culture of anaerobic cellulolytic bacteria.

    Science.gov (United States)

    Hernández-Díaz, R; Pimentel-González, D J; Figueira, A C; Viniegra-González, G; Campos-Montiel, R G

    2010-06-01

    In this work, the effect of a solid fungal culture of Aspergillus niger (An) grown on coffee pulp on the in situ ruminal degradability (RD) of corn stover was evaluated. In addition, the effect of its extracts on the in vitro dry matter disappearance (IVDMD) and on a mixed culture of anaerobic cellulolytic bacteria (MCACB) was also investigated. The solid ferment was a crude culture of An, grown on coffee pulp. Regarding in situ RD, a significant difference (p < 0.05) was found between treatment with 200 g/day of the solid culture and control (no solid culture added) on dry matter, crude protein and neutral detergent fibre on RD. All the water extracts (pH 4, 7 and 10) enhanced IVDMD and stimulated the cellulolytic activity on a MCACB. Ultrafiltration results showed that active compounds with a molecular weight lower than 30 kDa were responsible for the effect on MCACB. Such results suggest that the effects of the solid An culture in RD are related to the presence of water soluble compounds having a molecular weight lower than 30 kDa.

  9. Cost-effectiveness analysis of TOC removal from slaughterhouse wastewater using combined anaerobic-aerobic and UV/H2O2 processes.

    Science.gov (United States)

    Bustillo-Lecompte, Ciro Fernando; Mehrvar, Mehrab; Quiñones-Bolaños, Edgar

    2014-02-15

    The objective of this study is to evaluate the operating costs of treating slaughterhouse wastewater (SWW) using combined biological and advanced oxidation processes (AOPs). This study compares the performance and the treatment capability of an anaerobic baffled reactor (ABR), an aerated completely mixed activated sludge reactor (AS), and a UV/H2O2 process, as well as their combination for the removal of the total organic carbon (TOC). Overall efficiencies are found to be up to 75.22, 89.47, 94.53, 96.10, 96.36, and 99.98% for the UV/H2O2, ABR, AS, combined AS-ABR, combined ABR-AS, and combined ABR-AS-UV/H2O2 processes, respectively. Due to the consumption of electrical energy and reagents, operating costs are calculated at optimal conditions of each process. A cost-effectiveness analysis (CEA) is performed at optimal conditions for the SWW treatment by optimizing the total electricity cost, H2O2 consumption, and hydraulic retention time (HRT). The combined ABR-AS-UV/H2O2 processes have an optimal TOC removal of 92.46% at an HRT of 41 h, a cost of $1.25/kg of TOC removed, and $11.60/m(3) of treated SWW. This process reaches a maximum TOC removal of 99% in 76.5 h with an estimated cost of $2.19/kg TOC removal and $21.65/m(3) treated SWW, equivalent to $6.79/m(3) day.

  10. 口腔需氧菌及兼性厌氧菌种分离鉴定系统的建立%Isolation and identification of aerobic and facultative anaerobic bacteria in the oral cavity

    Institute of Scientific and Technical Information of China (English)

    陆文昕; 肖丽英; 李燕; 吴凡子; 周辛璇; 吴岚; 李明云; 任彪; 郭强; 黄睿洁; 李继遥

    2015-01-01

    目的:建立口腔需氧菌和兼性菌的系统分离鉴定方法。方法招募20名口腔健康志愿者及8名口腔疾病患者,分别采集唾液、龈下菌斑、龈上菌斑和根尖周肉芽样本,采用形态鉴定、全自动生化鉴定仪及16s rRNA基因测序的多相分类方法,对口腔可培养细菌进行分离鉴定。结果鉴定出口腔菌种63种,共175株,梅里埃生化鉴定与16s rRNA基因鉴定的符合度为22.39%,不符合的菌种以基因测序结果为准。链球菌属、放线菌属和葡萄球菌属检出率占前3位,菌种以咽峡炎链球菌、口腔放线菌、变异链球菌、缓症链球菌检出频率最高。慢性根尖周炎的咽峡炎链球菌检出率最高;放射性龋患者中,中间链球菌、金黄色葡萄球菌检出率较高;猛性龋患者中,变异链球菌远高于其他细菌。结论口腔需氧菌和兼性厌氧菌中以革兰阳性菌检出率最高。对于口腔细菌鉴定来说,梅里埃生化鉴定仪鉴定准确率不高,但可提供菌株生理生化指标,而全长的16s rRNA基因测序鉴定更为可靠。%Objective To establish a systematic method for isolation and identification of aerobic and facultative anaerobic bacteria in the oral cavity. Methods Samples of the saliva, dental plaque and periapical granulation tissue were collected from 20 subjects with healthy oral condition and from 8 patients with different oral diseases. The bacteria in the samples were identified by morphological identification, VITEK automatic microorganism identification and 16s rRNA gene sequencing. Results VITEK automatic microorganism identification and 16s rRNA gene sequencing showed an agreement rate of 22.39%in identifying the bacteria in the samples. We identified altogether 63 bacterial genus (175 species), among which Streptococcus, Actinomyces and Staphylococcus were the most common bacterial genus, and Streptococcus anginosus, Actinomyces oris, Streptococcus mutans

  11. Experiments on one-stage and two-stage anaerobic treatment of beetroot slop. Versuche zur ein- und zweistufigen anaeroben Behandlung von Ruebenmelasseschlempe

    Energy Technology Data Exchange (ETDEWEB)

    Fiebig, R.

    1987-12-17

    The experiments in this work, particularly in the sludge-bed process, confirm how extremely difficult anaerobic treatment of beetroot slop is. Use of the undiluted slop for the one-phase treatment in a sludge bed reactor mostly resulted only in the acidification of the biologically degradable materials, even if the retention time was more than 20 days. The high content of nitrogenous compounds, especially betaine, proved to be one of the biggest problems in the anaerobic treatment, probably along with the high content of salt and the presence of inhibiting Maillard products. Owing to concentrations of ammonia of, in part, more than 4 grams/litre resulting from the degradation of the nitrogenous compounds, firstly no properly grained, sedimentable sludge flakes were formed, and secondly, with a pH going up simultaneously to the range of 8.0, methane formation was considerably inhibited. In the two-phase process, again, a chemical oxygen consumption of more than 70% was achieved only if the slop was diluted in a ratio of at least 0.7 to 1; at the same time, a rise of the pH to over 7.5 had to be avoided. Since the acidification and the methane stages could not be optimized separately, the two-stage process, given a constant composition of the water, practically proved of no advantage.

  12. Anaerobic granular sludge and biofilm reactors

    DEFF Research Database (Denmark)

    Skiadas, Ioannis V.; Gavala, Hariklia N.; Schmidt, Jens Ejbye

    2003-01-01

    by the immobilization of the biomass, which forms static biofilms, particle-supported biofilms, or granules depending on the reactor's operational conditions. The advantages of the high-rate anaerobic digestion over the conventional aerobic wastewater treatment methods has created a clear trend for the change......-rate anaerobic treatment systems based on anaerobic granular sludge and biofilm are described in this chapter. Emphasis is given to a) the Up-flow Anaerobic Sludge Blanket (UASB) systems, b) the main characteristics of the anaerobic granular sludge, and c) the factors that control the granulation process...

  13. Mechanism of Kenaf Retting Using Aerobes

    Institute of Scientific and Technical Information of China (English)

    卢士森; 陈季华; 黄秀宝

    2001-01-01

    The experimental results showed that the duration of microbial retting processing of kenaf fibers by using aerobic microbe was four times shorter than that by using anaerobic microbe. The residual gum percentage,breaking strength, breaking elongation and linear density of aerobic retted kenaf bundle fibers did not show significantly difference with that of anaerobic retted kenaf bundle fibers by ANOVA-Tukey's studentized test at a = 5% except for the softness. The bioenergetic principle and the calculation of the amount of ATP produced during the decomposition processing of kenaf gums were used to explain why the retting duration in the case of using aerobic microbes was much shorter than that of using anaerobic microbes.

  14. Isolation and Cultivation of Anaerobes

    DEFF Research Database (Denmark)

    Aragao Börner, Rosa

    2016-01-01

    Anaerobic microorganisms play important roles in different biotechnological processes. Their complex metabolism and special cultivation requirements have led to less isolated representatives in comparison to their aerobic counterparts.In view of that, the isolation and cultivation of anaerobic...... microorganisms is still a promising venture, and conventional methodologies as well as considerations and modifications are presented here. An insight into new methodologies and devices as well as a discussion on future perspectives for the cultivation of anaerobes may open the prospects of the exploitation...

  15. Energy from anaerobic methane production. [Sweden

    Energy Technology Data Exchange (ETDEWEB)

    1982-02-01

    Since 1970 Swedish researchers have been testing the ANAMET (anaerobic-aerobic-methane) process, which involves converting industrial wastewaters via an initial anaerobic microbiological step followed by an aerobic one. Recycling the biomass material in each step allows shorter hydraulic retention times without decreasing stability or solids reduction. Since the first ANAMET plants began operating at a Swedish sugar factory in 1972, 17 more plants have started up or are under construction. Moreover, the ANAMET process has engendered to offshoot BIOMET (biomass-methane) process, a thermophilic anaerobic scheme that can handle sugar-beet pulp as well as grass and other soft, fast-growing biomasses.

  16. Investigations on the inactivation of selected bacteria and viruses during mesophilic and thermophilic anaerobic alkaline cofermentation of biological waste materials, food residues and other animal residues; Seuchenhygienische Untersuchungen zur Inaktivierung ausgewaehlter Bakterien und Viren bei der mesophilen und thermophilen anaeroben alkalischen Faulung von Bio- und Kuechenabfaellen sowie anderen Rest- und Abfallstoffen tierischer Herkunft

    Energy Technology Data Exchange (ETDEWEB)

    Hoferer, M. [Hohenheim Univ., Stuttgart (Germany). Inst. fuer Umwelt- und Tierhygiene sowie Tiermedizin mit Tierklinik

    2001-07-01

    The purpose of this study is to investigate the inactivation kinetics of a number of different bacteria (Salmonella Senftenberg, Escherichia coli O157, Enterococcus faecium) and viruses (Bovine Enterovirus (ECBO), Equine Rhinovirus (ERV), Poliovirus, Bovine Parvovirus (BPV)) during the process of anaerobic cofermentation. Experiments were conducted in a semi-technical biogas plant at the University of Hohenheim. The fermenter was fed with a mixture of slurry from pigs or cattle (75%) and leftovers (25%) and was run under mesophilic (30 C + 35 C) as well as under thermophilic temperature conditions (50 C + 55 C). Volume and filter-sandwich germ-carriers were specifically developed and/or optimised for these analyses. Parallel to the experiments at the University of Hohenheim and under almost identical process conditions, various viruses (African Swine Fever Virus, Pseudorabies Virus, Classical Swine Fever Virus, Foot and Mouth Disease Virus, Swine Vesicular Disease Virus) were examined at the Federal Research Centre for Virus Diseases of Animals in Tuebingen. The results obtained at each research institution are directly compared. (orig.)

  17. Anaerobic membrane bio-reactors for severe industrial effluents and urban spill waters: The AMBROSIUS project

    NARCIS (Netherlands)

    Van Lier, J.B.; Ozgun, H.; Ersahin, M.E.; Dereli, R.K.

    2013-01-01

    With growing application experiences from aerobic membrane bioreactors, combination of membrane and anaerobic processes become more and more attractive and feasible. In anaerobic membrane bioreactors (AnMBRs), biomass and particulate organic matter are physically retained inside the reactor, providi

  18. Comparison between traditional biological phosphorus removal by anaerobic/aerobic and denitrifying phosphorus removal by anaerobic/aerobic%传统厌氧/好氧生物除磷与厌氧/缺氧反硝化除磷效能的比较

    Institute of Scientific and Technical Information of China (English)

    苏婉琳; 韩萍芳

    2011-01-01

    采用序批式反应器(SBR),对比厌氧/好氧(A/O)和厌氧/缺氧(A/A)2种运行模式对模拟生活和工业混合污水同时脱氮除磷的效能.结果表明:反硝化聚磷菌完全可以在厌氧/缺氧交替运行条件下得到富集,稳定运行的2种模式对有机物和P的去除率分别保持在90%和85%以上,且A/A SBR具有更强的释磷能力,其释磷量比A/O SBR高出1.2倍.进一步试验表明:磷的释放在有无硝酸盐的情况下效果是不同的.2个系统内污泥均有反硝化除磷能力,A/A SBR中所含反硝化聚磷菌(DPAO)的比例是A/O SBR的4.56倍.2种模式出水水质都能取得较好的效果,且能实现同步除磷脱氮,而反硝化除磷在生物除磷方面更具优势.%Two sequence batch reactors(SBRs) in A/O and A/A run modes were operated in parallel to compare their ability of simultaneous phosphorus and nitrogen removal. The results showed that the ability of the anaerobic/anoxic strategy enriched the growth of denitrifying phosphorus bacteria capable of taking up phosphate under anoxic condition, and the removal of COD and phosphorus were averaged over 90% and 85% in the steady-state, respectively. A/A SBR had the stronger anaerobic phosphorus release ability; its amount of anaerobic released phosphorus is higher than 1. 2 times compared with A/O SBR. It is shown that interpretation of phosphorus release tests with or without nitrate present was different. The proportion of denitrifying phosphate accumulating organism ( DPAO) was 4. 56 times compared with the one of A/O SBR. The qualities of effluent were met the criteria, and DPAO systems could obtaia get simultaneous denitrifying and phosphorus removal. Thus, DPAO had more advantages than PAO for biological phosphorus removal.

  19. Degradation Action of the Anaerobic Bacteria and Oxygen to the Polymer

    Institute of Scientific and Technical Information of China (English)

    LU Xiang-Guo; ZHANG Ke

    2008-01-01

    Oxygen could prohibit anaerobic bacterium in the produced water and degrade the polymer molecular chains.Aiming at problems making up aerobic polymer solution by the produced water in Daqing Oil Field, some evaluations were done on the viscosity characteristics of polymer solution and bactericide in anaerobic and aerobic environments. Reasonable aerobic concentration of the produced water was obtained. The experimental results indicate that the viscosity of polymer solution confected by the produced water in the aerobic environment is higher than that of the polymer solution confected by the produced water in the anaerobic environment, and the reasonable ments, but the sterilization effect is better in the aerobic environment.

  20. Application of dynamic membranes in anaerobic membranes in anaerobic membrane bioreactor systems

    NARCIS (Netherlands)

    Erşahin, M.E.

    2015-01-01

    Anaerobic membrane bioreactors (AnMBRs) physically ensure biomass retention by the application of a membrane filtration process. With growing application experiences from aerobic membrane bioreactors (MBRs), the combination of membrane and anaerobic processes has received much attention and become m

  1. Nitrifying and denitrifying bacteria in aerobic granules formed in sequencing batch airlift reactors

    Institute of Scientific and Technical Information of China (English)

    WANG Fang; YANG Fenglin; QI Aijiu

    2007-01-01

    The purpose of this study was to investigate nitrifying bacteria and denitrifying bacteria isolated from aerobic granules.Aerobic granules were formed in an internal-circulate sequencing batch airlift reactor(SBAR)and biodegradation of NH3 -N was analyzed in the reactor.Bacteria were isolated and determined from aerobic granules using selected media.The growth properties and morphology of bacteria colonies were observed by controlling aerobic or anaerobic conditions in the culture medium.It was found that bacteria in aerobic granules were diverse and some of them were facultative aerobes.The diversity of bacteria in aerobic granules was a premise of simultaneous nitrification and denitrification.

  2. Schattenwirtschaft und Arbeitsplatzbeschaffung

    OpenAIRE

    Merz, Joachim

    1996-01-01

    Ziel dieser Studie ist die empirisch fundierte Auseinandersetzung mit dem Thema Schattenwirtschaft und Arbeitsplatzschaffung. Die hier aufgefächerte Diskussion des Themas in Wirtschaft, Sozialpolitik und Gesellschaft, der weitere Diskurs und die empirische Fundierung erfordern eine klare und dann folgende begriffliche Abgrenzung der Schattenwirtschaft. Argumente der Diskussion zu Ursachen und positiven und negativen Wirkungen der Schattenwirtschaft auf die Arbeitsplatzbeschaffung werden im An...

  3. Reductive Dechlorination of Trichloroethylene and Tetrachloroethylene under Aerobic Conditions in a Sediment Column

    OpenAIRE

    Enzien, Michael V.; Picardal, Flynn; Terry C Hazen; Arnold, R. G.; Fliermans, Carl B.

    1994-01-01

    Biodegradation of trichloroethylene and tetrachloroethylene under aerobic conditions was studied in a sediment column. Cumulative mass balances indicated 87 and 90% removal for trichloroethylene and tetrachloroethylene, respectively. These studies suggest the potential for simultaneous aerobic and anaerobic biotransformation processes under bulk aerobic conditions.

  4. Variation of EPS,enzyme activity,and bacteria density in sewage sludge during the anaerobic/aerobic digestion processes by ultrasonic sludge treatment%超声波-缺氧/好氧消化过程污泥EPS、酶活性及菌落数的变化研究

    Institute of Scientific and Technical Information of China (English)

    姚炜婷; 孙水裕; 郑莉; 尹光彩; 李楚华; 蔡明山; 许燕滨; 童文锦

    2011-01-01

    In order to investigate the sludge anaerobic/aerobic digestion mechanism by ultrasonic sludge treatment,the index proportion of EPS and dissolved substances(polysaccharides,protein and DNA),enzyme activities(protease activity and dehydorgenase activity) were measured in the anaerobic/aerobic digestion by the optimal ultrasonic treatment(28 kHz ultrasonic frequency,0.1 W·mL-1 ultrasonic density,15 min ultrasonic time and 12 h ultrasonic interval time).It showed that the performances of anaerobic/aerobic digestion were greatly improved by ultrasonic treatment,with the removal rate of VSS reaching 40.14% after digestion for 10 days,20% more than that of the controlled,and at the same time great amount of soluble organics was released.The index proportions of EPS and dissolved substances in the ultrasonic sludge and the control were almost the same,indicating that ultrasonic could promote oxygen/aerobic sludge digestion but would not change the index proportion of EPS and their dissolved substances.The variation trend of enzyme activities increased at the beginning and then dropped off after 10 days' digestion,with the maximum value appearing on the fourth day.The protease activity of ultrasonic sludge was more than that of the controlled during 0~5 d digestion,due to the markedly improved protease activity and the high-speed biological reaction.Organic matter degradation ratio was also improved greatly,leading to the improved VSS removal rate in ultrasonic sludge.While the variation of dehydrogenase activity in ultrasonic sludge was almost the same compared with the controlled,it was therefore unable to represent ultrasonic promotion by sludge anaerobic/aerobic digestibility.The variation trend of sludge bacteria density also increased at the beginning and then dropped off after 10 days' digestion,but it is controversial for the measurement of bacteria density.Further study is needed.%为研究超声波促进污泥缺氧/好氧消化的机理,采用

  5. 生活垃圾填埋场甲烷自然减排的新途径:厌氧与好氧的共氧化作用%A new way of natural mitigation of methane in a refuse landfill: Anaerobic and aerobic co-oxidation

    Institute of Scientific and Technical Information of China (English)

    韩丹; 石峰; 柴晓利; 陈浩泉; 赵由才

    2011-01-01

    The surface soil at 30 ~60 cm, refuse at 1.5 m depth and the aged refuse at the bottom of the refuse landfill were selected for experiments on sulfate-reducing bacteria (SRB). It was found that SRB existed in nearly all layers of the solid waste landfill, with aged refuse at the bottom containing the most and the surface soil the least SRB. Garbage samples with a 50%: 50% coarse:fine particle size proportion showed the best aerobic and anaerobic methane oxidation effect, and anaerobic oxidation accounted for above 20%. Microbial activity in aged refuse and co-oxidation rate of methane reached a maximum value with a 25% moisture content. Anaerobic oxidation rate could reach more than 30% as the moisture content approached 70%. Supplementation of exogenous methane could shorten the time of aerobic oxidation by 50%. However, both aerobic and anaerobic oxidation were weakened if the amount of exogenous methane was beyond 2 mL for the 20 g aged refuse. Therefore, a new way for natural mitigation of methane in a refuse landfill is put forward based on anaerobic and aerobic co-oxidation of methane.%采用暴雨过后垃圾填埋表层30~60 cm的覆土、表层1.5 m以下的垃圾,以及刚刚开挖出来的9年矿化垃圾进行硫酸盐还原菌阳性反应试验,结果表明生活垃圾填埋体不同填埋层都存在不同数量级的硫酸盐还原菌,且底层矿化垃圾中的硫酸盐还原菌的数量最多,表层覆土中最少.颗粒大小比例为50%:50%的垃圾样品表现出最佳的甲烷好氧与厌氧氧化效果,且厌氧氧化在共氧化作用中的比例达到20%以上.含水率为25%时,矿化垃圾中微生物活性最大,好氧与厌氧氧化甲烷速率均达到最大.当含水率接近70%时,甲烷厌氧氧化的贡献率可达30%以上.外源甲烷的补充可以驯化甲烷氧化微生物,其中甲烷好氧氧化时间最大可缩短50%;而初始甲烷一次通人量超过2 mL(20g矿化垃圾)后,甲烷好氧与厌氧氧化

  6. 生活垃圾填埋场甲烷自然减排的新途径——厌氧与好氧的共氧化作用%New Way for Natural Mitigation of Methane in Domestic Waste Landfill Sites: Co-oxidation of Anaerobic and Aerobic Oxidation

    Institute of Scientific and Technical Information of China (English)

    周海燕; 韩丹

    2011-01-01

    通过证实生活垃圾填埋场中甲烷厌氧氧化与好氧氧化的共存,提出了甲烷自然减排的新途径.分别选取暴雨过后垃圾填埋表层30~60 cm的覆土、1.5 m以下的垃圾以及底层矿化垃圾做硫酸盐还原菌阳性反应实验,结果表明:生活垃圾填埋体不同填埋层都存在不同数量级的硫酸盐还原菌,且底层矿化垃圾中的硫酸盐还原菌的数量最多,表层覆土中最少.颗粒大小比例为50%:50%的垃圾样品表现出最佳的甲烷好氧与厌氧氧化效果,且厌氧氧化在共氧化作用中的比例达到20%以上.含水率为25%时,矿化垃圾中微生物活性最大,好氧与厌氧氧化甲烷速率均达到最大;当含水率接近70%时,甲烷厌氧氧化的贡献率可达30%以上.外源甲烷的补充可以驯化甲烷氧化微生物,其中甲烷好氧氧化时间最大可缩短50%;而甲烷通入量超过2 mL后,甲烷好氧与厌氧氧化均受到抑制.%A new way for natural mitigation of methane was put forward by authenticating co-oxidation of anaerobic and aerobic oxidation of methane in domestic waste landfill sites. The soil at 30-60 cm, the waste below 1.5 m from the surface, and the aged waste at the bottom, were selected for the experiments of sulfate-reducing bacteria positive reaction. The results showed that sulfate-reducing bacteria nearly existed in all landfill layers of waste landfill bodies, and aged waste at the bottom contained most, the surface soil contained least. Waste samples with 50%: 50% of coarse and fine particle size proportion showed the best methane oxidation effect of aerobic and anaerobic oxidation, and anaerobic oxidation accounted for above 20%. Microbial activity in aged waste and its methane co-oxidation rate both reached the maximum value as moisture content was 25%. Anaerobic oxidation rate could reach more than 30% as moisture content was close to 70%. Supplement of exogenous methane could culture methane-oxidizing bacteria

  7. Effect of High Intensity Interval Training (HIIT) on Aerobic And Anaerobic Endurance in Young Man%短期高强度间歇运动训练对青年男性有氧耐力和无氧耐力的影响

    Institute of Scientific and Technical Information of China (English)

    王正

    2015-01-01

    Objective:To investigate the effects of HIIT on aerobic and anaerobic endurance in young man. Methods:Before operation,30 subjects underwent cardiopulmonary exercise test( CEPT) with incremental protocol to measure the peak power( PP) . Then the 30 subjects were randomly divided into high-intensity ( 95%of PP ) interval training group ( n =10 ) , moderate-intensity ( 75%of PP ) training group ( n = 10 ) and low-intensity ( 50% of PP ) training group( n=10 ) . HIIT performed by the mode of one mi-nutes training separated by one minute of rest, MIT and LIT accepted persistent training under set intensity,every group lasted 30 minutes each time. Before and after train-ing,all patients received cardiopulmonary exercise testing ( CPET) and Wingate test to assess their aerobic and an-aerobic endurance. Results:before training, three groups of subjects had no significantly difference ( p>0. 05 ) in-VO2 peak、anaerobic threshold ( AT )、peak power ( PP )、power drop ( PD )、average power ( AP );after training, there were significant differences(p <0. 05)in VO2peak and AT in three groups,and HIIT is the most significant;but there were significant differences ( p <0. 05 ) in AP, DP and PP only in HIIT. Conclusion:Short term of HIIT, MIT and LIT can improve the aerobic endurance,howev-er, only HIIT has significant influence on anaerobic en-durance.%目的::探讨短期的不同强度运动训练对青年男性有氧耐力和无氧耐力的影响。方法:将30名符合纳入标准的青年男性志愿者进行运动心肺测试测得峰值功率等数据,并且随机分为高强度间歇运动训练组( HIIT组)、中等强度持续训练组( MIT组)、低强度持续训练组( LIT 组)。 HIIT 组取峰值功率的95%,以1min运动1min休息的形式进行,MIT组和LIT组分别取峰值功率的75%和50%,以持续运动的方式进行,所有受试者每次训练30min,隔日1次,共进行6周。对受试者训练前后有氧耐力指标最大摄氧量(VO2max)、无

  8. Diskurs und Praxis: Geschlecht und Gewalt

    Directory of Open Access Journals (Sweden)

    Angela Koch

    2003-11-01

    Full Text Available Der Sammelband befasst sich kulturwissenschaftlich mit den Verschränkungen der diskursiven Repräsentationen von Geschlecht und Gewalt, mit deren alltäglichen Erscheinungsformen und juristischen Auslegungen. Im Vordergrund steht die personale Gewalttätigkeit als physisches Mittel des Zwangs, der Dominanz und der Macht. Der Band geht auf die Tagung „Frauen und Gewalt“ zurück, die im Oktober 2001 in Greifswald stattgefunden hat, und versammelt 18 Beiträge, die unterschiedliche Facetten des Themenkomplexes behandeln: Gewalt gegen Frauen wie Inzest, Vergewaltigung, Folter, Anprangerung im Internet, und weibliche Gewalttätigkeit wie Verbrechen, Mord, Kindsmord, physische Gewalt.

  9. Winery and distillery wastewater treatment by anaerobic digestion.

    Science.gov (United States)

    Moletta, R

    2005-01-01

    Anaerobic digestion is widely used for wastewater treatment, especially in the food industries. Generally after the anaerobic treatment there is an aerobic post-treatment in order to return the treated water to nature. Several technologies are applied for winery wastewater treatment. They are using free cells or flocs (anaerobic contact digesters, anaerobic sequencing batch reactors and anaerobic lagoons), anaerobic granules (Upflow Anaerobic Sludge Blanket--UASB), or biofilms on fixed support (anaerobic filter) or on mobile support as with the fluidised bed. Some technologies include two strategies, e.g. a sludge bed with anaerobic filter as in the hybrid digester. With winery wastewaters (as for vinasses from distilleries) the removal yield for anaerobic digestion is very high, up to 90-95% COD removal. The organic loads are between 5 and 15 kgCOD/m3 of digester/day. The biogas production is between 400 and 600 L per kg COD removed with 60 to 70% methane content. For anaerobic and aerobic post-treatment of vinasses in the Cognac region, REVICO company has 99.7% COD removal and the cost is 0.52 Euro/m3 of vinasses.

  10. Toxicants inhibiting anaerobic digestion: a review.

    Science.gov (United States)

    Chen, Jian Lin; Ortiz, Raphael; Steele, Terry W J; Stuckey, David C

    2014-12-01

    Anaerobic digestion is increasingly being used to treat wastes from many sources because of its manifold advantages over aerobic treatment, e.g. low sludge production and low energy requirements. However, anaerobic digestion is sensitive to toxicants, and a wide range of compounds can inhibit the process and cause upset or failure. Substantial research has been carried out over the years to identify specific inhibitors/toxicants, and their mechanism of toxicity in anaerobic digestion. In this review we present a detailed and critical summary of research on the inhibition of anaerobic processes by specific organic toxicants (e.g., chlorophenols, halogenated aliphatics and long chain fatty acids), inorganic toxicants (e.g., ammonia, sulfide and heavy metals) and in particular, nanomaterials, focusing on the mechanism of their inhibition/toxicity. A better understanding of the fundamental mechanisms behind inhibition/toxicity will enhance the wider application of anaerobic digestion.

  11. What Is Aerobic Dancing?

    Science.gov (United States)

    ... than 24 million people participate in aerobics. Aerobic dance has blossomed into a sport for all to have fun while losing weight and keeping in shape. There are varied forms of aerobics including low-impact, swim and other water aerobics is popular. Dance is still the predominant form and may be ...

  12. Anaerobic bacteria

    Science.gov (United States)

    Brook I, Goldstein EJ. Diseases caused by non-spore forming anaerobic bacteria. In: Goldman L, Schafer AI, eds. Goldman's Cecil Medicine . 25th ed. Philadelphia, PA: Elsevier Saunders; 2015:chap 297. Stedman's Online ...

  13. Experimental Study on the Effects of Swiss Ball Core Strength Training on Aerobic and Anaerobic Exercise Ability%瑞士球核心力量训练对有氧和无氧运动能力影响的实验研究

    Institute of Scientific and Technical Information of China (English)

    赵敬国; 赵龙

    2012-01-01

    To evaluate the effects of Swiss ball core strength training and traditional ground strength training on improving college students' aerobic and anaerobic exercise ability. The subjects in core strength training group (n = 13) did push - up and curl - up on Swiss Ball (GoFit Swiss Ball, 75 cm) , and those in traditional strength training group (n = 7) did push - up and curl - up on the ground; The subjects in the control group (n = 8) didn't do any related special training. Subjects in both strength training group did exercises for 7 weeks,3 times/week,60 min/time, 60 s each type with 2 s/shot, 3 groups each type, a total of 6 groups, 5 min intervals between groups. We performed Wingate anaerobic testing and maximal oxygen uptake measurement before and after training. There were no significant difference (P>0.05) in all indexes before and after training in control group. In the core strength training group and traditional strength training group, comparing with the value before the training, the absolute and the relative values of maximal oxygen uptake were significantly increased ( P < 0. 01) ; the peak anaerobic power were significantly increased ( P < 0. 05 ) . The average anaerobic power was very significantly increased (P<0.01) in the core strength training group, and it was significantly increased(P < 0. 05) in the traditional strength training group. The core strength training and the traditional strength training had no significant difference in improving aerobic and anaerobic exercise ability.%进一步评价瑞士球核心力量训练和传统地面力量训练对大学生有氧和无氧运动能力的增进效益.核心力量训练组(n=13)采用瑞士球(GoFit Swiss Ball,75 cm)上俯卧撑和瑞士球上仰卧起坐;传统力量训练组(n=7)采用地上俯卧撑和仰卧起坐;对照组受试者(n=8)不进行任何相关的专门性训练.力量训练组每周训练3次,每次60 min,每个动作持续60 s,节拍器控制节奏2s/拍,每种方式3

  14. Physikdidaktik Theorie und Praxis

    CERN Document Server

    Girwidz, Raimund; Häußler, Peter

    2015-01-01

    „Physikdidaktik – Theorie und Praxis“ ist ein Sammelband, der dynamisch gewachsen ist. Der Teil I wurde im Jahre 2000 in erster Linie für Studierende des Lehramts Physik konzipiert. Der Teil II zeigt Konkretisierungen und im Unterricht erprobte Beispiele zu neueren didaktischen und methodischen Ansätzen, die aus der Pädagogik und der allgemeinen Didaktik für die Physikdidaktik aufbereitet wurden. Die Physikdidaktik befasst sich natürlich auch mit der Frage, welche Elemente aus der modernen Physik in den Unterricht eingehen können und sollen. Vor der Aufbereitung der Inhalte für den Unterricht, mit Vereinfachungen und angemessenen didaktischen Reduktionen, steht die Sachanalyse und die Zusammenfassung von interessanten Themen aus aktuellen experimentellen und theoretischen Arbeitsgebieten der Physik (Teil III). In der vorliegenden 3. Ausgabe eines Gesamtbandes „Physikdidaktik – Theorie und Praxis“ wurden Astrophysik, Elementar­teilchenphysik und Biophysik als interessante Beispiele aus ...

  15. Frauen und Sucht : Literaturkompendium

    OpenAIRE

    Franke, Alexa; Mohn, Karin; Welbrink, Andrea; Witte, Maibritt; Teuber, Jutta

    2000-01-01

    Das vorliegende Literaturkompendium stellt die aktuelle wissenschaftliche Literatur zu dem Thema "Frauen und Sucht" systematisch und möglichst umfassend dar. Es beinhaltet Literaturhinweise zu frauenspezifischen Ursachen, Bedingungen, Verläufen und Folgen des Substanzmißbrauchs. Zur besseren Überschaubarkeit und um den Umfang der Recherche einzugrenzen, bezieht sich das Kompendium ausschließlich auf folgende Substanzen: Alkohol, Medikamente und illegale Drogen. Grundlage des Kompendiums ...

  16. Form und Leben zwischen Positivismus und Idealismus

    Directory of Open Access Journals (Sweden)

    Kai Nonnenmacher

    2015-02-01

    Full Text Available Der Artikel skizziert bei u.a. Dilthey, Hofmannsthal, Rilke, Bergson, Vossler und Croce in vier Schritten den Zusammenhang zwischen der Lebensphilosophie und der idealistischen Neuphilologie, die Frage poetischer Autonomie mit diesem spezifischen Wissensbegriff um 1900 verknüpfend:1. Antipositivismus als Folie eines neuen Wissensbegriffs um 1900;2. Wissensschau einer lebensphilosophischen Poetik;3. Autonomie und Leben;4. Diskursgeschichte literarischer Autonomie.Der Artikel geht zurück auf einen Vortrag bei der von der Thyssen-Stiftung geförderten Tagung „Kunst, Erkenntnis, Wissenschaft (techne und episteme, ars und scientia“ von Marion Hiller, Hochschule Vechta, 2011. 

  17. Anaerobic Metabolism and Bioremediation of Explosives-Contaminated Soil

    Science.gov (United States)

    Boopathy, Raj

    Nitroaromatic compounds pollute soil, water, and food via use of pesticides, plastics, pharmaceuticals, landfill dumping of industrial wastes, and the military use of explosives. Biotransformation of trinitrotoluene and other nitroaromatics by aerobic bacteria in the laboratory has been frequently reported, but the anaerobic bacterial metabolism of nitroaromatics has not been studied as extensively perhaps due to the difficulty in working with anaerobic cultures and the slow growth of anaerobes. Sulfate-reducing and methanogenic bacteria can metabolize nitroaromatic compounds under anaerobic conditions if appropriate electron donors and electron acceptors are present in the environment.

  18. 不同年龄段女子足球运动员 Yo - Yo间歇恢复性测试与有氧、无氧能力的关联研究%Correlation Between Yo - Yo Intermittent Recovery Test and Aerobic,Anaerobic Capacity of Women Soccer Players in Different Age Groups

    Institute of Scientific and Technical Information of China (English)

    孙哲; 陈效科; 丰保成; 徐萌

    2016-01-01

    Fifty - two athletes from the Chinese National Women’soccer team of U - 12,U - 15,U - 19 were chosen as experimental subjects,and the relationship among Yo - Yo IR1,Yo - Yo IR2,aerobic,anaerobic capacity of different ages women’football athletes through VO 2 max,average anaerobic power testing were analyzed. Corre-lation analysis showed that the Yo - Yo IR1,Yo - Yo IR2 test results were in low correlation with VO 2 max test results,and was in high correlation with the average anaerobic power results of women’football athletes in the U - 12 group;there is a moderate correlation between Yo - Yo IR1,Yo - Yo IR2 and average anaerobic power results in U - 15 group,but athletes’VO 2 max has no obvious correlation with Yo - Yo IR1,Yo - Yo IR2; there is a significant correlation between Yo - Yo IR1,Yo - Yo IR2 test results and VO 2 max results,and there is no correlation between the two and average anaerobic power. The results show that Yo - Yo intermittent re-covery test can evaluate the pubescent women soccer players’anaerobic capacity,and adult women soccer play-ers’aecrobic capacity.%本研究以中国国家女子足球队 U -12、U -15、U -19集训队伍的52名运动员为实验对象,分别进行了 Yo - Yo IR1、Yo - Yo IR2、最大摄氧量、平均无氧功率测试,以分析 Yo - Yo 间歇恢复性测试与不同年龄段女子足球运动员有氧、无氧能力的关系。相关性分析表明:(1)U -12年龄组女子足球运动员 Yo - Yo IR1、Yo - Yo IR2测试与最大摄氧量测试结果之间低度相关,而与平均无氧功率结果间呈高度相关;(2)U -15年龄组运动员 Yo - Yo IR1、Yo - Yo IR2和平均无氧功率结果间中度相关,而2者和运动员的最大摄氧量没有明显的相关关系;(3)U -19年龄组运动员 Yo - Yo IR1、Yo - Yo IR2测试成绩和最大摄氧量结果间显著相关,而与平均无氧功率间关联程度很弱。结论:Yo - Yo间歇

  19. Information und Kommunikation

    Science.gov (United States)

    Wesoly, Michael; Ohlhausen, Peter; Bucher, Michael; Hichert, Rolf; Korge, Gabriele; Schnabel, Ulrich; Gairola, Arun; Reichwald, Ralf; Habicht, Hagen; Möslein, Kathrin; Schwarz, Torsten; Schönsleben, Paul; Scherer, Eric; Schloske, Alexander; Adlbrecht, Gerald; Federhen, Jens

    Wissen ist mittlerweile unverzichtbar für den entscheidenden Vorsprung auf dem Markt. Unabhängig davon, ob sich das Wissen in Innovationen, neuen Produkten und Dienstleistungen oder in der Verbesserung interner Unternehmensprozesse manifestiert: Wissen ist Treiber von Innovation, Wissen bringt Schnelligkeit und Wissen ist die Voraussetzung für Problemlösungen [40]. Wissensmanagement ist daher der Schlüssel für Unternehmenserfolg und bezeichnet den "bewussten und systematischen Umgang mit der Ressource Wissen und den zielgerichteten Einsatz von Wissen in der Organisation“ [6]. In dieser umfassenden Sichtweise ist Wissensmanagement ein Begriff, der Konzepte, Strategien und Methoden umfasst.

  20. Westliche und muslimische Geschlechter?

    Directory of Open Access Journals (Sweden)

    Stanislawa Paulus

    2008-11-01

    Full Text Available Mihçiyazgan geht der Frage nach wie Differenzen in männlichen und weiblichen Subjektkonstutionen im Westen und im Islam empirisch erfasst werden können. Hierbei verfolgt sie eine antiessentialitische Perspektive, in der sie sich zentral auf Judith Butler und Michel Foucault bezieht. Über beide hinausgehend entwickelt sie ein Modell pluraler Diskurse, mit dessen Hilfe kulturelle bedingte Geschlechterkonstruktionen verstehbar werden. Anhand einer Untersuchung von Interviews, in der sie interaktionsanalytische und diskursanalytische Herangehensweisen verbindet, macht sie unterschiedliche Zonen des Sagbaren und Unsagbaren in westlichen und muslimischen Geschlechterdiskursen sichtbar.

  1. Ingenieurgeologie: Grundlagen und Anwendung

    Science.gov (United States)

    Genske, Dieter D.

    Geplünderter Planet? Die Ingenieurgeologie ist ein junges, interdisziplinäres Fachgebiet. Es befasst sich mit der Wechselwirkung zwischen Geosphäre und Mensch. Der Autor stellt Georisiken vor und diskutiert die Folgen menschlicher Eingriffe in die Natur. Er thematisiert aktuelle Herausforderungen und entwirft nachhaltige Lösungskonzepte für verschiedene Problembereiche: z.B. Naturgefahren wie Hangrutschungen und Bergfälle, die Ausbeute natürlicher Ressourcen, Bau- und Sanierungsvorhaben. Fallbeschreibungen und ausgearbeitete Beispiele vertiefen das Verständnis für diesen neuen, zukunftsweisenden Zweig der Wissenschaft.

  2. VAPOR PHASE TREATMENT OF PCE IN A SOIL COLUMN BY LAB-SCALE ANAEROBIC BIOVENTING

    Science.gov (United States)

    Microbial destruction of highly chlorinated organic compounds must be initiated by anaerobic followed by aerobic dechlorination. In-situ dechlorination of vadose zone soil contaminated with these compounds requires, among other factors, the establishment of highly reductive anaer...

  3. 厌氧MBBR-好氧MBR组合工艺对PU合成革废水的除氮试验研究%Experimental research on the removal of nitrogen from PU synthetic leather wastewater by combined process anaerobic MBBR and aerobic MBR

    Institute of Scientific and Technical Information of China (English)

    王庆; 丁原红; 任洪强; 刘敏敏; 郝立志; 杨倩

    2012-01-01

    he biochemical effluent water from Fuyang Xingye Synthetic Leather Co., Ltd., is characterized by comparatively low COD,high nitrogen content,and wide fluctuation range. The biochemical combined process anaerobic MBBR-aerobic MBR has continuously been used for treating synthetic leather wastewater for four months, in Fuyang Xingye Synthetic Leather Co., Ltd., Hangzhou. The experimental results indicates that its COD can steadily reach the mark,and the effluent ammonium nitrogen are steadily maintained between 7.46-13.82 mg/L,as well,meeting the requirements of the Discharge Standard of Pollutants from Synthetic Leather and Artificial Leather Industry (GB 21902-008).%杭州富阳兴业合成革有限公司的生化工艺出水COD相对较低,含氮量较高,且波动较大.采用厌氧MBBR-好氧MBR组合工艺对该废水进行了4个月的连续处理试验,结果表明:除COD可稳定达标外,出水氨氮也可稳定维持在7.46~13.82 mg/L之间,达到《合成革与人造革工业污染物排放标准》(GB 21902-2008)要求.

  4. Evaluation of Biodegradability of Waste Before and After Aerobic Treatment

    Directory of Open Access Journals (Sweden)

    Suchowska-Kisielewicz Monika

    2014-12-01

    Full Text Available An important advantage of use of an aerobic biostabilization of waste prior to its disposal is that it intensifies the decomposition of the organic fraction of waste into the form which is easily assimilable for methanogenic microorganisms involved in anaerobic decomposition of waste in the landfill. In this article it is presented the influence of aerobic pre-treatment of waste as well as leachate recirculation on susceptibility to biodegradation of waste in anaerobic laboratory reactors. The research has shown that in the reactor with aerobically treated waste stabilized with recilculation conversion of the organic carbon into the methane is about 45% higher than in the reactor with untreated waste stabilized without recirculation.

  5. Strahlungsmessung und Dosimetrie

    CERN Document Server

    Krieger, Hanno

    2013-01-01

    „Strahlungsquellen und Dosimetrie“ ist Teil einer Lehrbuchreihe zur Strahlungsphysik und zum Strahlenschutz. Der erste Teil befasst sich mit den physikalischen Grundlagen der Strahlungsdetektoren und der Strahlungsmessung. Im zweiten Teil werden die Konzepte und Verfahren der klinischen Dosimetrie dargestellt. Der dritte Abschnitt erläutert ausführlich die Dosisverteilungen der klinisch angewendeten Strahlungsarten. Im vierten Teil werden weitere Messaufgaben der Strahlungsphysik einschließlich der Messsysteme für die Bildgebung mit Röntgenstrahlung dargestellt. Neben den grundlegenden Ausführungen enthält dieser Band im laufenden Text zahlreiche Tabellen und Grafiken zur technischen und medizinischen Radiologie, die bei der praktischen Arbeit sehr hilfreich sein können und 199 Übungsaufgaben mit Lösungen zur Vertiefung der Inhalte. Für die zweite Auflage wurden die Darstellungen der Elektronen- und der Protonendosimetrie sowie der bildgebenden Verfahren mit Computertomografen deutlich erweit...

  6. Aerobic and anaerobic mineralization of Salvinia molesta and Myriophyllum aquaticum leachates from a tropical reservoir (Brazil Mineralização aeróbia e anaeróbia dos lixiviados de Salvinia molesta e Myriophyllum aquaticum de um reservatório tropical (Brasil

    Directory of Open Access Journals (Sweden)

    Renato Henriques da Silva

    2011-06-01

    Full Text Available AIM: This study aimed at describing and discussing the leachates mineralization (aerobic and anaerobic of two species of aquatic macrophytes (Salvinia molesta and Myriophyllum aquaticum from a tropical reservoir (22° 00' S and 47° 54' W; METHODS: The incubations were prepared with plant leachates and reservoir water sample and were maintained during 45 days in the dark (at 20 °C. The organic carbon and the oxygen consumption kinetics were evaluated; RESULTS: Irrespective of to the experimental condition, the leachates were mainly utilized for catabolic processes (i.e., respiration, mineralization was slightly faster in an aerobic environment (1.22 fold and in this condition, the yield of refractory products was smaller (2.3%; the O/C stoichiometric ratios values (oxygen consumed per atom of carbon from mineralization of the 2 types of leachates were similar (ca. 1.12; CONCLUSIONS: According to these results we conclude that the leachate from selected macrophytes is rapidly decomposed and subsidize primariy the microbial catabolism (aerobic or anaerobic; in addition, we propose that S. molesta contributes more to the input of dissolved organic matter within the reservoir.OBJETIVO: Nesse estudo foram descritas as mineralizações (aeróbias e anaeróbias dos lixiviados de 2 espécies de macrófitas aquáticas (Salvinia molesta e Myriophyllum aquaticum que ocorrem em um reservatório tropical (22° 00' S e 47° 54' O; MÉTODOS: As incubações, preparadas com os lixiviados das plantas e amostra de água do reservatório, foram mantidas no escuro e a 20 °C, por 45 dias. Foram avaliadas as cinéticas de perda de massa do carbono orgânico e de consumo de oxigênio; RESULTADOS: Independente da condição experimental, os lixiviados foram principalmente utilizados na respiração; as mineralizações foram ligeiramente mais rápidas em meio aeróbio (1,22 vezes e nessa condição, os rendimentos dos produtos refratários foram menores (2,3%; as

  7. Tratamento de águas residuárias de suinocultura em reatores anaeróbios horizontais seguidos de reator aeróbio em batelada sequencial Swine waste water treatment in horizontal anaerobic reactor followed by aerobic sequencing batch reactor

    Directory of Open Access Journals (Sweden)

    Ariane C. dos Santos

    2011-01-01

    Full Text Available Avaliou-se o desempenho de um sistema combinado anaeróbio-aeróbio para o tratamento de águas residuárias de suinocultura, com concentrações médias de sólidos suspensos totais (SST de 18.624 e 11.395 mg L-1. Foram utilizados quatro reatores anaeróbios horizontais com volume total de 49,5 L cada, um com manta de lodo (RAHML e três de leito fixo (RAHLF, instalados em série e seguidos de um reator aeróbio operado em batelada sequencial (RBS com volume total de 339 L e com alimentação contínua. Nos RAHLF, foram utilizados como meios suporte de anéis de bambu, anéis plásticos de eletroduto corrugado e anéis de bucha (Luffa cillyndrica, respectivamente. Os tempos de detenção hidráulica (TDH e as cargas orgânicas volumétricas (COV aplicadas no RAHML foram de 12 e 10 h e 53 e 61 g DQO (L d-1, respectivamente. O RBS foi operado com ciclo de 24 h e COV de 0,34 e 0,50 g DQO (L d-1. As eficiências médias de remoção de DQOtotal e SST para o conjunto de reatores anaeróbios horizontais, em série, foram de 96 e 99%, e de 96 e 95%, respectivamente. As maiores produções volumétricas de metano ocorreram nos RAHLF, com valores médios de até 0,744 m³ CH4 (m³ reator d-1. A inclusão do RBS permitiu melhorar a qualidade do efluente e a estabilidade do sistema de tratamento, atingindo eficiências de remoção de DQOtotal e SST de 99 e 99%, e de 98 e 99%, respectivamente. No RBS, ocorreu nitrificação e desnitrificação, com remoções de N-amoniacal de até 65%.An anaerobic-aerobic combined system was evaluated to treat swine wastewater with an average total suspended solids (TSS concentration of 18,624 to 11,395 mg L-1. The anaerobic treatment system was constituted by four horizontal reactors each one having a total volume of 49.5 L, one with sludge blanket (HASBR and tree with fixed film (HAFFR installed in series, followed by an aerobic sequential batch reactor (SBR with continue feeding, with total volume of 339 L. In the

  8. 果糖和麦芽糖作为有氧碳源对大肠杆菌NZN111两阶段发酵中厌氧发酵的影响%Effects of fructose and maltose as aerobic carbon sources on subsequently anaerobic fermentation by Escherichia coli NZN111

    Institute of Scientific and Technical Information of China (English)

    吴辉; 李志敏; 叶勤

    2011-01-01

    To understand the effects of sugar whose uptake is dependent or independent on the phosphotransferase system (PTS), two-stage culture of Escherichia coli strain NZN111 that was constructed by disruption of idhA and pfIB encoding the fermentative lactate dehydrogenase (LDH) and pyruvate: formate lyase (PFL) of E. Coli W1485, was carried out for organic acids production. When NZN111 was aerobically cultured on fructose (PTS dependent) or maltose (PTS independent), it fermented glucose with succinic acid and pyruvic acid as the major products in subsequent anaerobic culture. The experiments were also performed in a 5-L fermentor. The yields of succinic acid by the fructose-and maltose-grown NZN111 were 0.84 and0.75 mol/mol, whereas the yields of pyruvic acid were 0.65 and 0.83 mol/mol, respectively. The final ratio of succinic acid to pyruvic acid in the anaerobic stage reached 1.73:1 and 1.21:1, respectively. The different behaviors in anaerobic fermentation by the fructose-, maltose- and glucose-grown NZN111 were likely caused by the regulation of catabolite repression in the aerobic culture stage.%为了了解磷酸转移酶转运系统(PTS)依赖和非PTS依赖代谢的糖类对大肠杆菌生产琥珀酸的影响,进行了两阶段培养,有氧阶段采用PTS依赖型的果糖或非PTS依赖型的麦芽糖作为丙酮酸甲酸裂解酶(PFL)和乳酸脱氢酶(LDH)双突变株NZN111的碳源,研究其对NZN111厌氧阶段代谢葡萄糖的影响.5L罐发酵结果表明,以果糖和麦芽糖为碳源有氧培养的细胞恢复了在厌氧条件下快速代谢葡萄糖的能力,琥珀酸和丙酮酸成为主要代谢产物,最终琥珀酸得率分别为0.84和0.75 mol/mol,丙酮酸得率分别达到了0.65和0.83 mol/mol,琥珀酸和丙酮酸终浓度比分别为1.73:1和1.21:1.果糖和麦芽糖培养的NZN111与葡萄糖培养的菌体代谢的明显差异推测是cyclic AMP (cAMP)依赖型和非cAMP依赖型的分解代谢物阻遏调控这两种机制共同作用的结果.

  9. Anaerobic treatment of wastewater. Application for food industry; Depuracion anaerobia del aguas residuales. Se aplicacion en la industria alimentaria

    Energy Technology Data Exchange (ETDEWEB)

    Carceller Rosa, J. M.

    2005-07-01

    Activated sludge aerobic reactors is a wide spread system in waste water treatment plants. Excessive proliferations of filamentous microorganisms give rise to bulking and foaming problems. Wastewater from food and drink industries as well as paper mill and related industries has severe risk of bulking episodes. Incorporation of anaerobic pre-treatment previous to existing aerobic treatment previous to existing aerobic treatment avoids bulking problems. Anaerobic systems are therefore indicated in waste waters with high concentrations of ready biodegradable organic substrates, such us waste waters from breweries, distilleries, soft drinks, paper mill industries, vegetable processing industries, etc. Basic principles of anaerobic wastewater treatment are exposed in this paper, with special reference to the most wide spread anaerobic systems: UASB, EGSB e IC reactors. Operational parameters of anaerobic and aerobic systems are compared, as well as investment and management costs, including biogas recovery. (Author) 7 refs.

  10. [Formation Mechanism of Aerobic Granular Sludge and Removal Efficiencies in Integrated ABR-CSTR Reactor].

    Science.gov (United States)

    Wu, Kai-cheng; Wu, Peng; Xu, Yue-zhong; Li, Yue-han; Shen, Yao-liang

    2015-08-01

    Anaerobic Baffled Reactor (ABR) was altered to make an integrated anaerobic-aerobic reactor. The research investigated the mechanism of aerobic sludge granulation, under the condition of continuous-flow. The last two compartments of the ABR were altered into aeration tank and sedimentation tank respectively with seeded sludge of anaerobic granular sludge in anaerobic zone and conventional activated sludge in aerobic zone. The HRT was gradually decreased in sedimentation tank from 2.0 h to 0.75 h and organic loading rate was increased from 1.5 kg x (M3 x d)(-1) to 2.0 kg x (M3 x d)(-1) while the C/N of 2 was controlled in aerobic zone. When the system operated for 110 days, the mature granular sludge in aerobic zone were characterized by compact structure, excellent sedimentation performance (average sedimentation rate was 20.8 m x h(-1)) and slight yellow color. The system performed well in nitrogen and phosphorus removal under the conditions of setting time of 0.75 h and organic loading rate of 2.0 kg (m3 x d)(-1) in aerobic zone, the removal efficiencies of COD, NH4+ -N, TP and TN were 90%, 80%, 65% and 45%, respectively. The results showed that the increasing selection pressure and the high organic loading rate were the main propulsions of the aerobic sludge granulation.

  11. Medikamentengabe und Psychotherapie

    OpenAIRE

    2013-01-01

    Psychopharmakotherapie ist ein möglicher Baustein in der multimodalen Behandlung kinder- und jugendpsychiatrischer Störungsbilder. Diese simple Aussage ist unumstritten. Dennoch haben im klinischen Alltag sowohl Eltern als auch Psychotherapeuten häufig erhebliche Bedenken, einem Kind ein Psychopharmakon zu verabreichen. Neben angemessenen Bedenken zu potenziellen Wechselwirkungen zwischen psychopharmakotherapie und Psychotherapie oder Unsicherheiten zu Wirkungen und Nebenwirkungen eines Psych...

  12. Digital Zeichnen und Malen

    Directory of Open Access Journals (Sweden)

    Christoph Kaindel

    2013-12-01

    Full Text Available Digitales Zeichnen und Malen hat gegenüber dem „analogen“ Vorteile wie auch Nachteile. In der folgenden, unvollständigen Liste stelle ich die technischen Vor- und Nachteile, die mir aufgefallen sind, gegenüber. Kommentare und Ergänzungen sind willkommen!

  13. Anaerobic depuration of waste waters; Depuracion anaerobia de aguas residuales

    Energy Technology Data Exchange (ETDEWEB)

    Mejias Sanchez, G.; Vazquez Berger, E.; Magana Pietra, A.H. [Facultad de Ingenieria, Universidad Autonoma de yucatan, Merida (Mexico)

    1996-08-01

    Trials were carried out at a 500 l semi-experimental plant using there reactor models-anaerobic filter, fixed film and UASB type-for the anaerobic treatment of waste from different sources. The results after 24 and 48 hours were compared. The greatest efficiency was obtained after 48 hours the aerobic filter reactor (66% displacement), followed by the fixed film reactor (50%) and the UASB model (41%). (Author) 16 refs.

  14. ANAEROBIC BIOLOGICAL TREATMENT OF PRODUCED WATER

    Energy Technology Data Exchange (ETDEWEB)

    John R. Gallagher

    2001-07-31

    During the production of oil and gas, large amounts of water are brought to the surface and must be disposed of in an environmentally sensitive manner. This is an especially difficult problem in offshore production facilities where space is a major constraint. The chief regulatory criterion for produced water is oil and grease. Most facilities have little trouble meeting this criterion using conventional oil-water separation technologies. However, some operations have significant amounts of naphthenic acids in the water that behave as oil and grease but are not well removed by conventional technologies. Aerobic biological treatment of naphthenic acids in simulated-produced water has been demonstrated by others; however, the system was easily overloaded by the large amounts of low-molecular-weight organic acids often found in produced waters. The objective of this research was to determine the ability of an anaerobic biological system to treat these organic acids in a simulated produced water and to examine the potential for biodegradation of the naphthenic acids in the anaerobic environment. A small fixed-film anaerobic biological reactor was constructed and adapted to treat a simulated produced water. The bioreactor was tubular, with a low-density porous glass packing material. The inocula to the reactor was sediment from a produced-water holding pond from a municipal anaerobic digester and two salt-loving methanogenic bacteria. During start-up, the feed to the reactor contained glucose as well as typical produced-water components. When glucose was used, rapid gas production was observed. However, when glucose was eliminated and the major organic component was acetate, little gas was generated. Methane production from acetate may have been inhibited by the high salt concentrations, by sulfide, or because of the lack, despite seeding, of microbes capable of converting acetate to methane. Toluene, a minor component of the produced water (0.1 g/L) was removed in the

  15. NADH-linked aldose reductase: the key to anaerobic alcoholic fermentation of xylose by yeasts

    NARCIS (Netherlands)

    Bruinenberg, P.M.; De Bot, P.H.M.; Van Dijken, J.P.; Scheffers, W.A.

    1984-01-01

    The kinetics and enzymology of o-xylose utilization were studied in aerobic and anaerobic batch cultures of the facultatively fermentative yeasts Candida utilis, Pachysolen tannophilus, and Pichia stipitis. These yeasts did not produce ethanol under aerobic conditions. When shifted to anaerobiosis c

  16. Effect Of Imposed Anaerobic Conditions On Metals Release From Acid-Mine Drainage Contaminated Streambed Sediments

    Science.gov (United States)

    Remediation of streams influenced by mine-drainage may require removal and burial of metal-containing bed sediments. Burial of aerobic sediments into an anaerobic environment may release metals, such as through reductive dissolution of metal oxyhydroxides. Mining-impacted aerob...

  17. Romanistik und gender studies

    Directory of Open Access Journals (Sweden)

    Susanne Schlünder

    2000-11-01

    Full Text Available Die beiden Bände bieten ein breites Spektrum von Beiträgen zur französischen, italienischen und spanischen Literaturwissenschaft. Gedankliche Grundlage der im einzelnen unterschiedlichen Ansätze und Zielsetzungen ist ein im Anschluß an Judith Butler gender-reflektierendes, diskursives Konzept von Geschlecht, dessen wissenschaftsgeschichtliche Herleitung und Perspektiven Renate Kroll einleitend darlegt. Die einzelnen Artikel beschäftigen sich zum einen mit literarischen Strategien, die Schriftstellerinnen vom Mittelalter bis zur Gegenwart erprobt haben, und hinterfragen dabei die Rolle weiblicher Autoren in Literaturgeschichte und Literaturgeschichtsschreibung. Zum anderen widmen sie sich den literarischen Inszenierungs- und Repräsentationsformen von Weiblichkeit und stellen darüber einen Bezug zur Lebenswelt der behandelten Autorinnen her.

  18. Anaerobic biodegradability of macropollutants

    DEFF Research Database (Denmark)

    Angelidaki, Irini

    2002-01-01

    A variety of test procedures for determination of anaerobic biodegradability has been reported. This paper reviews the methods developed for determination of anaerobic biodegradability of macro-pollutants. Anaerobic biodegradability of micro-pollutants is not included. Furthermore, factors import...

  19. Schwangerschaftshypertonie: Therapie und Nachsorge

    Directory of Open Access Journals (Sweden)

    Homuth V

    2008-01-01

    Full Text Available Kurzfassung: Hypertensive Schwangerschaftserkrankungen komplizieren mehr als 10 % aller Schwangerschaften weltweit. Klinisch am bedeutsamsten sind die durch Hypertonie und Proteinurie nach der 20. Schwangerschaftswoche definierte schwangerschaftsinduzierte Präeklampsie und Pfropfpräeklampsie, insbesondere deren schwerste Verlaufsformen Eklampsie und HELLP-Syndrom. Sie gehören global unverändert zu den führenden Ursachen für fetale, neonatale und mütterliche Morbidität und Mortalität. Ihre Ätiologie ist unbekannt und daher ist mit Ausnahme der Beendigung der Schwangerschaft eine kausale Therapie nicht möglich. Insbesondere bleibt eine antihypertensive Therapie hinsichtlich der fetalen Entwicklung problematisch und sollte zur Vermeidung mütterlicher kardiovaskulärer Komplikationen erst bei Blutdruckwerten ≥ 170/110 mmHg begonnen werden, bei vorbestehendem Hochdruck oder Pfropfkonstellation (präexistente Nierenerkrankung, Diabetes mellitus bereits ab Blutdruckwerten von ≥ 160/ 100 mmHg. Zu beachten ist dabei die nur geringe Auswahl in der Schwangerschaft einsetzbarer Antihypertensiva, die sich im Wesentlichen auf Methyldopa, und bereits eingeschränkt auf den Betablocker Metoprolol sowie Dihydralazin und den Kalziumantagonisten Nifedipin begrenzt. Eine der wichtigsten Aufgaben nach der Entbindung ist die Gewährleistung und Förderung des Stillens. Aufgrund ausreichender medikamentöser Alternativen ist ein Abstillen wegen einer antihypertensiven Therapie nicht indiziert. Ergebnisse aktueller Untersuchungen zeigen, dass eine Hypertonie in der Schwangerschaft und ihre Komplikationen Frühgeburtlichkeit und vermindertes Geburtsgewicht einen bedeutsamen Risikofaktor für das Auftreten kardiovaskulärer Erkrankungen, wie Herzinfarkt und Schlaganfall, im späteren Leben darstellen. Daher sind für davon Betroffene frühzeitige Maßnahmen zur kardiovaskulären Prävention und lebenslangen Verlaufskontrolle zu fordern.

  20. Efeitos do exercício aeróbico e anaeróbico em variáveis de risco cardíaco em adultos com sobrepeso Effects of aerobic and anaerobic exercise on cardiac risk variables in overweight adults

    Directory of Open Access Journals (Sweden)

    Mônica Medeiros Moreira

    2008-10-01

    Full Text Available FUNDAMENTO: O exercício físico aeróbico é importante aliado no combate aos fatores de risco cardiovascular. No entanto, os efeitos de exercícios de alta intensidade sobre tais fatores ainda são pouco conhecidos. OBJETIVO: Comparar os efeitos de protocolos de exercícios aeróbico e anaeróbico sobre fatores associados ao risco cardíaco. MÉTODOS: Vinte e dois indivíduos com idade média de 40±8 anos foram alocados nos grupos: controle (CO, treinamento de endurance (ET e treinamento intermitente (IT. Os protocolos tiveram duração de 12 semanas, três vezes por semana; e intensidades de 10% abaixo e 20% acima do limiar anaeróbico (LAn. Foram medidas: massa corporal total (MCT, índice de massa corporal (IMC, circunferências de cintura (CINT e quadril (QUA e a composição corporal, além das concentrações plasmáticas de glicose (GLI, colesterol total (CHO e triglicérides (TG; ainda foram calculados a razão cintura-quadril (PCCQ e o índice de conicidade (Índice C. RESULTADOS: As variáveis de MCT, IMC, CINT, GLI e a composição corporal apresentaram alterações significativas nos grupos ET e IT. Os valores de CHO e QUA foram significativamente reduzidos no grupo ET, enquanto a PCCQ mostrou redução significativa no grupo IT. O LAn e o índice C, no grupo IT foram significativamente diferentes em relação a ET. CONCLUSÃO: Tendo em vista as diferenças encontradas nas respostas das variáveis estudadas, em razão do treinamento empregado, concluímos que um programa de exercício que contemple atividades de alta e baixa intensidades seja mais completo para garantir a redução de maior número de variáveis de risco cardíaco.BACKGROUND: Aerobic exercise is an important ally in the fight against cardiovascular risk factors. However, the effects of high-intensity exercise on these factors are still poorly known. OBJECTIVE: To compare the effects of aerobic and anaerobic exercise protocols on cardiac risk factors. METHODS: 22

  1. 好氧反硝化菌AD-1处理大豆乳清废水厌氧出水的性能评价%Performance Assessment on An Aerobic Denitrifier AD-1 Treating Soybean Whey Wastewater Anaerobic Effluent

    Institute of Scientific and Technical Information of China (English)

    蔡广潞; 吴培; 张国宁; 吴世晗

    2011-01-01

    To increase nitrogen removal efficiency, the study screened a high-efficiency aerobic denitrifier AD-1 in the activated sludge originated from a university previous designed CAAC (Continuous Aerobic-anaerobic Coupled)reactor treating SWWAE (Soybean Whey Wastewater Anaerobic Effluent).The strain was identified as pseudomonas putida according to the 16S rDNA sequence homology comparison and phyletic evolution analysis.The denitrification efficiency of Pseudomonas putida AD-1 was analyzed in DM medium feeding nitrate as the sole nitrogen source by shake-flask batch culture.After 48h the TN and nitrate removal rate reached 63.31% and 63.35%, respectively.Nitrite concentration was always at low level but fluctuated.At the later culture stage the low COD/TN ratio was one of main rate-limiting factors.AD-1 had high-efficiency denitrification with 100% nitrate removal rate in the process of treating SWWAE.Results indicated thai AD-1 was a good bioaugmentation agent for SWWAE and other nitrogenous wastewater treatment.%为了提高污水中氮素的去除效率,本研究自某大学微生物实验室开发的处理大豆乳清废水厌氧出水的CAAC(Continuous Aerobicanaerobic Coupled)反应器污泥中筛选出l株高效好氧反硝化菌AD-1,经16S rDNA序列同源性比较和系统发育分析初步鉴定为恶臭假单胞菌(pseudomonas putida).通过摇瓶批次培养考察菌株AD-1对以硝酸盐为唯一氮源的DM反硝化培养基的脱氮性能.48h时AD-1的TN和N03-N去除率分别达到63.31%和63.35%,N02-N浓度虽始终处于较低水平却呈高低波动状态,培养后期CODcr/TN下降是限制AD-1反硝化效率的主要因素之一.AD-1处理大豆乳清废水厌氧出水时NO3-N去除率接近100%,具有较高效的反硝化特性.结果表明,该菌株可作为处理大豆乳清废水厌氧出水及其它含氮废水的生物强化剂.

  2. ANAEROBIC MEMBRANE BIOREACTORS FOR DOMESTIC WASTEWATER TREATMENT. PRELIMINARY STUDY

    Directory of Open Access Journals (Sweden)

    Luisa Vera

    2014-12-01

    Full Text Available The operation of submerged anaerobic membrane bioreactors (SAnMBRs for domestic wastewaters treatment was studied in laboratory scale, with the objective to define sustainable filtration conditions of the suspensions along the process. During continuous experiments, the organic matter degradation by anaerobic way showed an average DQOT removal of 85% and 93%. Indeed, the degradation generated biogas after 12 days of operation and its relative methane composition was of 60% after 25 days of operation. Additionally, the comparison between membrane bioreactors (MBRs performance in aerobic and anaerobic conditions in filterability terms, reported that both systems behave similarly once reached the stationary state.

  3. Degradation of municipal solid waste in simulated landfill bioreactors under aerobic conditions.

    Science.gov (United States)

    Slezak, Radoslaw; Krzystek, Liliana; Ledakowicz, Stanislaw

    2015-09-01

    In this study the municipal solid waste degradation processes in simulated landfill bioreactors under aerobic and anaerobic conditions is investigated. The effect of waste aeration on the dynamics of the aerobic degradation processes in lysimeters as well as during anaerobic processes after completion of aeration is presented. The results are compared with the anaerobic degradation process to determine the stabilization stage of waste in both experimental modes. The experiments in aerobic lysimeters were carried out at small aeration rate (4.41⋅10(-3)lmin(-1)kg(-1)) and for two recirculation rates (24.9 and 1.58lm(-3)d(-1)). The change of leachate and formed gases composition showed that the application of even a small aeration rate favored the degradation of organic matter. The amount of CO2 and CH4 released from anaerobic lysimeter was about 5 times lower than that from the aerobic lysimeters. Better stabilization of the waste was obtained in the aerobic lysimeter with small recirculation, from which the amount of CO2 produced was larger by about 19% in comparison with that from the aerobic lysimeter with large leachate recirculation.

  4. Geschlechtsrolle und psychische Erkrankung

    Directory of Open Access Journals (Sweden)

    Möller-Leimkühler AM

    2005-01-01

    Full Text Available Aus der Perspektive der historisch/gesellschaftlich konstruierten sozialen Geschlechtsrollen wird anhand sozialepidemiologischer Daten die Frage erörtert, welche Zusammenhänge zwischen den traditionellen Geschlechtsrollen und dem Auftreten psychischer Störungen bestehen. Die Geschlechtsrolle stellt ein zentrales Bindeglied zwischen personaler und sozialer Identität sowie gesellschaftlichen Bedingungen dar. Hinter den geschlechtsspezifisch unterschiedlichen Störungsprofilen bei Männern und Frauen verbirgt sich eine Reihe von Risikofaktoren, die jeweils andere Konsequenzen für die Geschlechter haben. Diese Risikofaktoren sind einerseits mit der sozialen Lage verknüpft (Rollenvielfalt vs. Rollenreduktion, Arbeitslosigkeit, berufliche Gratifikationskrisen, Ehe/Trennung, andererseits entstehen sie über handlungsrelevante soziale Stereotypisierungen ("Gender bias"“ in medizinischen Institutionen. Gesundheitsrisiken sind nicht nur extern mit der Geschlechtsrolle verbunden, sondern können sich auch über die Internalisierung traditioneller Weiblichkeits- und Männlichkeitsnormen entwickeln, die zu geschlechtsspezifischen Verarbeitungsmustern psychosozialer Belastungen führen. So fördern geschlechtstypische Gesundheits- und Krankheitskonzepte, geschlechtstypische Belastungskonstellationen und Defizite normativer Sozialisation bei Frauen eher affektive und psychosomatische Störungen, bei Männern eher Alkoholabhängigkeit, Persönlichkeitsstörungen, Gewaltdelikte und Suizid. Die dargestellten Zusammenhänge zwischen sozialem Geschlecht und psychischen Störungen haben zahlreiche Implikationen für eine geschlechtersensible Forschung, Therapie und Gesundheitspolitik.

  5. Preliminary cleaning of brewery waste water in a two-stage anaerobic plant: influence of COD in the inflow on cleaning efficiency and biogas formation; Vorreinigung von Brauereiabwasser in zweistufigen Anaerob-Anlagen: Einfluss des CSB im Zulauf auf die Reinigungsleistung und Biogasbildung

    Energy Technology Data Exchange (ETDEWEB)

    Herrmann, A.P. [Universitaet des Saarlandes, Saarbruecken (Germany). Lehrstuhl fuer Prozesstechnik; Janke, H.D. [Gesellschaft fuer Umweltkompatible Prozesstechnik mbH (upt), Saarbruecken (Germany); Chmiel, H. [Gesellschaft fuer Umweltkompatible Prozesstechnik mbH (upt), Saarbruecken (Germany); Universitaet des Saarlandes, Saarbruecken (Germany). Lehrstuhl fuer Prozesstechnik

    1999-07-01

    Using a continuously operated, two-stage laboratory system (acidification reactor and packed-bed methane reactor) and with brewery waste water as a substrate, systematic studies concerning the influence of COD{sup inflow} on fatty acid formation, COD reduction and biogas formation were carried out. In the upshot, the executed pilot tests permit the conclusion that treatment of a partial stream (COD{sup inflow} {>=} 5000mg/l), though not advantageous in terms of space/time yield, may be more economical on the whole under certain boundary conditions than treatment of the entire stream (COD{sup inflow} 1800-3000 mg/l). (orig.) [German] Mit einer kontinuierlich betriebenen, zweistufigen Laboranlage (Versaeuerungsreaktor und Festbett-Methanreaktor) wurden unter Verwendung von Brauereiabwasser als Substrat systematische Untersuchungen zum Einfluss des CSB{sup ZULAUF} auf die Fettsaeurebildung, CSB-Reduktion und Biogasbildung durchgefuehrt. Aus den durchgefuehrten Modellversuchen laesst sich zusammenfassend ableiten, dass eine Teilstrombehandlung (CSB{sup ZULAUF}{>=}5.000 mg/l) zwar hinsichtlich der Raum/Zeit-Ausbeute keine Vorteile mit sich bringt, aber unter bestimmten Randbedingungen insgesamt wirtschaftlicher als eine Vollstrombehandlung (CSB{sup ZULAUF} 1.800-3.000 mg/l) sein kann. (orig.)

  6. Photonik: Grundlagen, Technologie und Anwendung

    Science.gov (United States)

    Hering, Ekbert; Martin, Rolf

    Photonik gilt als eine der Zukunftstechnologien mit starkem Bezug zu zahlreichen Technologiefeldern. Dem trägt das Buch Rechnung. Nach einer Einführung in die physikalischen und technischen Grundlagen geben die Autoren einen Einblick in die Fertigung optischer Komponenten und Systeme. Es folgen optische Sensortechnik und Meßtechnik und die Anwendungen in Informations- und Kommunikationstechnik sowie in der Produktionstechnik, in der Medizin und im Konsumgüterbereich.

  7. Aerobic rice: crop performance and water use efficiency

    Directory of Open Access Journals (Sweden)

    Chiara Grassi

    2011-11-01

    Full Text Available Rice (Oryza sativa production largely depends on traditional flooded rice systems whose sustainability is threatened by a progressive decrease in water availability and a constant increase in rice demand due to strong demographic boom in world population. A newly developed water-saving rice system is aerobic rice in which rice grows in nonflooded and unsaturated soil. From 2001, at the International Rice Research Institute in the Philippines, this system has been monitored to identify potentially promising varieties of rice able to grow as an irrigated upland crop and quantify yield potential and water use efficiency. This study reports on the results of cultivating the upland rice variety Apo under different water conditions in 2004-2005 at the IRRI farm in both the dry and wet seasons. The water treatments considered were: aerobic and flooded conditions, alternated flooded and aerobic conditions and aerobic after fallow. Yield and water productivity were compared between aerobic and flooded treatment in both seasons, with the objective of analysing the differences between water treatments. In the experiment the effect of different nitrogen (N application is also considered. The results indicate that the aerobic rice yield was lower than rice production under flood treatment, confirming that observed over past years. Nevertheless, when the aerobic condition is alternated with the anaerobic condition, or a fallow period, the production under aerobic treatment provides good yields (respectively 4.2 and 4.4 ha-1. The fallow period was introduced to observe the response of rice grown under this management. Water productivity was higher in aerobic fields, especially after fallow (0.88 g kg-1. The nitrogen application induced an increase in yield and water productivity, partially compensating for the lack of water in aerobic fields.

  8. Anaerobic Thermophiles

    Directory of Open Access Journals (Sweden)

    Francesco Canganella

    2014-02-01

    Full Text Available The term “extremophile” was introduced to describe any organism capable of living and growing under extreme conditions. With the further development of studies on microbial ecology and taxonomy, a variety of “extreme” environments have been found and an increasing number of extremophiles are being described. Extremophiles have also been investigated as far as regarding the search for life on other planets and even evaluating the hypothesis that life on Earth originally came from space. The first extreme environments to be largely investigated were those characterized by elevated temperatures. The naturally “hot environments” on Earth range from solar heated surface soils and water with temperatures up to 65 °C, subterranean sites such as oil reserves and terrestrial geothermal with temperatures ranging from slightly above ambient to above 100 °C, to submarine hydrothermal systems with temperatures exceeding 300 °C. There are also human-made environments with elevated temperatures such as compost piles, slag heaps, industrial processes and water heaters. Thermophilic anaerobic microorganisms have been known for a long time, but scientists have often resisted the belief that some organisms do not only survive at high temperatures, but actually thrive under those hot conditions. They are perhaps one of the most interesting varieties of extremophilic organisms. These microorganisms can thrive at temperatures over 50 °C and, based on their optimal temperature, anaerobic thermophiles can be subdivided into three main groups: thermophiles with an optimal temperature between 50 °C and 64 °C and a maximum at 70 °C, extreme thermophiles with an optimal temperature between 65 °C and 80 °C, and finally hyperthermophiles with an optimal temperature above 80 °C and a maximum above 90 °C. The finding of novel extremely thermophilic and hyperthermophilic anaerobic bacteria in recent years, and the fact that a large fraction of them belong

  9. Psychiatrische Erkrankungen und Wirtschaftskrisen

    Directory of Open Access Journals (Sweden)

    Berghofer G

    2014-01-01

    Full Text Available Es besteht ein enger Zusammenhang von wirtschaftlichen Krisen und erhöhten Raten an Arbeitslosigkeit mit psychischen Erkrankungen wie Depressionen, Angstzuständen, Schlafstörungen und Alkoholismus sowie erhöhten Suizidraten. Gleichzeitig gibt es wirkungsvolle Maßnahmen, diesen schädlichen Gesundheitsauswirkungen von Wirtschaftskrisen effektiv entgegenzuwirken. Dazu gehören soziale und finanzielle Absicherungsprogramme, arbeitsmarktpolitische Förderangebote, Unterstützungsmaßnahmen für Familien und Entschuldungsprogramme. In Wirtschaftskrisen vorgenommene Kürzungen im Gesundheitswesen führen hingegen zu einer Verschlechterung der gesundheitlichen Versorgung und längerfristig zu erhöhten Gesundheitskosten. Um negativen gesundheitlichen Auswirkungen von Wirtschaftskrisen effektiv vorzubeugen, sollte daher präventiv in den Ausbau psychosozialer Angebote und medizinischer wie therapeutischer Versorgungseinrichtungen mit niederschwelligem Zugang investiert werden.

  10. Ethnografische Diskursanalyse und Sozialwissenschaften

    OpenAIRE

    Macgilchrist, Felicitas; Van Hout, Tom

    2011-01-01

    Ethnografie und Diskursanalyse werden in den Sozialwissenschaften zunehmend kombiniert. In diesem Beitrag wird zunächst ein Überblick gegeben über das entstehende Feld der mit den Epistemologien und Methoden der Ethnografie und Diskursanalyse arbeitenden Forschung. Im zweiten Schritt werden zentral Aspekte eines neuen, computergestützten Ansatzes zur ethnografischen Diskursanalyse vorgestellt. Dieser mikroanalytische Ansatz bietet die Möglichkeit, Erkenntnisse über das Ringen um Deutungsmuste...

  11. Hodenbiopsie, Histologie und TESE

    Directory of Open Access Journals (Sweden)

    Bergmann M

    2007-01-01

    Full Text Available Die Spermatogenese erfolgt in den Keimtubuli, die aus einer Lamina propria und dem Keimepithel bestehen. Das Keimepithel besteht aus Keimzellen und somatischen Sertoli-Zellen. Sertoli-Zellen triggern die Spermatogenese über die hormonellen Stimuli von FSH und Androgenen und bilden die Blut-Hoden-Schranke, die Spermatozyten und Spermatiden vor dem körpereigenen Immunsystem schützt und so eine Autoimmunreaktion verhindert. Die Spermatogenese umfaßt die Proliferation der Spermatogonien, die Meiose der Spermatozyten und die Differenzierung der Spermatiden in Spermatozoen (Spermiogenese. Man unterscheidet I–VI verschiedene Assoziationen von Keimzellen – "Stadien der Spermatogenese" –, die in einem "multi-stage arrangement" in der Längsachse der Keimtubuli angeordnet sind. Verschiedene Spermatogenesedefekte in benachbarten Keimtubuli: "Bunte Atrophie" ist assoziiert mit Differenzierungsdefiziten der Sertoli-Zellen. Biopsien sollten in Bouinscher Lösung fixiert werden. Die Diagnose des Carcinoma in situ erfolgt durch den immunhistochemischen Nachweis der plazentalen alkalischen Phosphatase, die ausschließlich von den CISZellen exprimiert wird. Die histologische Evaluierung erfolgt über ein "score count"-Verfahren und die Applikation moderner histologischer Techniken zum Protein- und mRNA-Nachweis. Der sonographische Befund einer Mikrolithiasis korreliert histologisch mit dem Auftreten intratubulärer sphärischer Konkremente, einer Verdickung der Lamina propria und eines Tubulusschattens. Er ist nicht spezifisch für CIS/TIN, korreliert aber mit CIS/TIN in einem kleinen Subkollektiv von Patienten und bestätigt das erhöhte Risiko infertiler Patienten, einen Hodentumor zu entwickeln. Bei Klinefelter- Patienten besteht die Chance einer fokalen spermatogenetischen Restfunktion, die die Möglichkeit einer TESE/ICSI-Therapie eröffnet. Die Hodenbiopsie sollte nur unter Einhaltung strikter Kriterien für Indikation und histologische Evaluation in

  12. Marktmanipulation und Compliance

    OpenAIRE

    Blumenberg, Axel-Dirk

    2016-01-01

    Die Arbeit behandelt zunächst den Tatbestand der Marktmanipulation aus kriminologischer und wirtschaftlicher Sicht. Im Anschluss wird der damit verbundene Straftatbestand aus der Perspektive des deutschen, spanischen und europäischen Rechts analysiert. Dabei werden auch kriminalpolitische Aspekte, wie die Privatisierung des Strafrechts, die "regulierte Selbstregulierung" und das europäische Gesetzgebungsverfahren berücksichtigt. Im zweiten Teil der Arbeit werden die Möglichkeiten der ...

  13. Demographischer Wandel und Tourismus

    OpenAIRE

    Reuber, Paul; Wolkersdorfer, Günter

    2006-01-01

    In vielen Regionen Deutschlands spielt der Tourismus- und Freizeitmarkt eine bedeutende Rolle. Der Tourismus bietet den Zieldestinationen bezüglich der räumlichen Auswirkungen des demographischen Wandels nicht nur Risiken, sondern durchaus auch erhebliche Chancen. Die wichtigsten Veränderungen des demographischen Wandels für Freizeit und Tourismus betreffen die Gruppe der „Jungen Erwachsenen“ und die Gruppe der „Älteren Menschen“ (50plus-Generation). Beide Gruppen sind durch di...

  14. Geoinformation und Staat

    Science.gov (United States)

    Zypries, Brigitte

    2002-09-01

    Geoinformationen besaßen als Grundlage militärischer Interventionen schon immer einen hohen Stellenwert für den Staat. Im Zeitalter der modernen Informations- und Kommunikationstechnologien wächst in demokratischen Staaten die Bedeutung von Geodaten weit über den Verteidigungsbereich hinaus. Brigitte Zypries, Staatssekretärin im Bundesministerium des Innern (BMI) und Vorsitzende des Interministeriellen Ausschusses für Geoinformationswesen (IMAGI), sprach mit Dipl.-Geogr. Ute C. Bauerüber die Anwendung und Koordinierung von Geodaten in Politik und Staat.

  15. Autonomes Fahren und Stadtstruktur

    OpenAIRE

    2015-01-01

    Mobilität, Verkehr und die physische Gestalt städtischer Räume sind eng miteinander verknüpft [1]. Die Stadtstruktur bildet eine wichtige Grundlage für Mobilitätsentscheidungen von Haushalten und Unternehmen und gibt in entscheidendem Maße vor, welche Formen von Verkehr ermöglicht oder aber auch ausgeschlossen werden. Kompakte Stadtstrukturen mit hoher Dichte und Nutzungsmischung bieten gute Voraussetzungen für kurze Wege, ein leistungsfähiges öffentliches Verkehrsangebot, fördern den Fuß- un...

  16. Virtuelle und ideale Welten

    OpenAIRE

    Bertrand, Cyrille-Paul; Cremers, Martin; Erben, Dietrich; Gehmann, Ulrich; Gerbing, Chris; Gleitsmann, Rolf-Jürgen; Guggenberger, Bernd; Holmer, Sebastian; Krämer, Steffen; Kunze, Rolf-Ulrich; Oetzel, Günther; Schmidt, Michael; Schulze, Ulrich

    2017-01-01

    Technik und technischer Wandel zählen zu jenen Faktoren, die unser (all-)tägliches Leben entscheidend prägen. Dieser Sachverhalt dürfte in unserem technischen Zeitalter kaum einer besonderen Begründung bedürfen. Es liegt auf der Hand, dass die Menschheit von Technik und technischem Fortschritt abhängig geworden ist, und dies nicht erst in unserer Zeit. Seit jeher war es der Technik entwickelnde und zielgerichtet einsetzende Mensch, der vermittels ,seiner‘ Technik Lebensräume gestaltete, verä...

  17. Digital Natives und Datenschutz

    Directory of Open Access Journals (Sweden)

    Triz Heider

    2010-04-01

    Full Text Available Dieser Artikel lädt dazu ein, sich ein Bild der virtuellen Welt zu machen, ihre eingeborenen User zu verstehen, ihre Sprache und Kultur kennen zu lernen und damit einen Schritt zur Integration in „die digitale Gesellschaft“ (falls es sie gibt zu tun. Es findet eine Auseinandersetzung über die aktuelle Entwicklung des Internets, notwendige Kompetenzen Hilfesuchender und die Architektur von virtueller Beratung statt. Aspekte von Datenschutz und Privatsphäre runden die Ausführungen ab. Dabei werden sowohl aktuelle Themen angesprochen, als auch Vergleiche mit anderen, großen Internetangeboten wie SocialNetworks herangezogen.

  18. Influência do treinamento aeróbio e anaeróbio na massa de gordura corporal de adolescentes obesos Influencia del entrenamiento aeróbico y anaeróbico en la masa grasa corporal de adolescentes obesos Influence of the aerobic and anaerobic training on the body fat mass in obese adolescents

    Directory of Open Access Journals (Sweden)

    Ana Cláudia Fernandez

    2004-06-01

    porcentaje de grasa; y el ejercicio aeróbico fue más eficaz en el sentido de preservar y/o aumentar la masa magra y la masa libre de grasa.The aim of this study was to verify the influences of anaerobic and aerobic exercise in the body composition of obese male adolescents. The sample was constituted of 28 adolescents with ages ranging from 15 and 19 years, and severe obesity. The volunteers were divided into three groups and for all groups was provided nutritional orientation. The intervention period was 12 weeks. Group I accomplished interval training in a cycle ergometer that consisted of 12 shots of 30 sec. with maximum power and speed, pedaling with high load (0.8% of the body weight x 25 watts and 3 min recover. Group II accomplished aerobic training in a cycle ergometer pedaling with relative load to the ventilatory threshold for 50 min. Group III served as control, and was not submitted to physical activity. All volunteers underwent bone densitometry with analysis of the body composition (DEXA and medical and fitness evaluations. For the variables weight and BMI exercise groups presented reductions when initial and final interventions periods were compared. Regarding to the body composition, there was a decrease in total body and lower limbs fat mass (in grams and in the fat percentage of the lower limbs in the exercise groups when initial and final periods were compared. There was a significant difference between groups I and II for the percentile deltas of total body fat mass and of lower limbs and in the percentage of fat of lower limbs. The data suggest that anaerobic and aerobic exercise with nutritional orientation promoted a larger weight reduction, when compared with nutritional orientation only. The anaerobic exercise proposed in this study was more efficient to produce reduction of body fat and fat percentage. The aerobic exercise seemed to have been more effective preserving and/or increasing free fat mass.

  19. Virulenzregulationskaskade und Chitobiose-Metabolismus in Vibrio cholerae

    OpenAIRE

    Berg, Thorsten

    2008-01-01

    Vibrio cholerae, der Erreger der gastrointestinalen Erkrankung Cholera, ist ein Gram- negatives, fakultativ anaerobes gekrümmtes Stäbchenbakterium und zugleich der wohl bekannteste Vertreter der Familie Vibrionaceae. Es persisitiert die meiste Zeit in aquatischen Ökosystemen wie Flüssen, Seen oder Meeresküsten, wo das Bakterium meist mit Crustaceen oder anderen Organismen mit Chitin-haltigen Oberflächen assoziiert vorliegt. Über orale Aufnahme kontaminierter Lebensmittel oder von Wasser kann ...

  20. Epilepsie und psychiatrische Erkrankungen

    Directory of Open Access Journals (Sweden)

    Baumgartner C

    2008-01-01

    Full Text Available Psychiatrische Erkrankungen treten bei Epilepsiepatienten signifikant häufiger auf als in der Allgemeinbevölkerung und als bei Patienten mit anderen chronischen Erkrankungen. Die Depression stellt die häufigste psychiatrische Begleiterkrankung bei Epilepsie dar. Die Häufigkeit von Depressionen korreliert mit der Anfallskontrolle: Sie liegt zwischen 3 und 9 % bei gut kontrollierter Epilepsie, jedoch zwischen 20 und 55 % bei Patienten mit therapieresistenten Epilepsien. Umgekehrt ist bei Patienten mit neu diagnostizierten Epilepsien anamnestisch signifikant häufiger eine Depression zu erheben als in einem Vergleichskollektiv. Diese bidirektionale Beziehung zwischen Epilepsie und Depression könnte durch gemeinsame Pathomechanismen beider Erkrankungen erklärt werden. Obwohl das Vorliegen und der Schweregrad einer Depression die wichtigsten Prädiktoren für die Lebensqualität bei Epilepsiepatienten darstellen, werden Depressionen bei Epilepsiepatienten unterdiagnostiziert und unterbehandelt. Eine psychopharmakologische Behandlung sollte bei Vorliegen einer Begleitdepression deshalb unverzüglich initiiert werden, das epileptogene Potential von Antidepressiva stellt dabei ein vernachlässigbares Risiko dar. Die Prävalenz psychotischer Störungen bei Epilepsiepatienten liegt zwischen 2 und 8 %, wobei sogenannte episodische Psychosen (iktale, postiktale und Alternativpsychosen, die in einem zeitlichen Bezug zum Anfallsgeschehen stehen, und chronische Psychosen (interiktale Psychosen ohne zeitlichen Bezug zu den Anfällen, unterschieden werden können. Die Prävalenz von Angststörungen bei Epilepsiepatienten liegt zwischen 15 und 25 %. Man kann zwischen präiktaler, iktaler, postiktaler und interiktaler Angst unterscheiden.

  1. Wissensmanagement und Medienbildung - neue Spannungsverhältnisse und Herausforderungen

    Directory of Open Access Journals (Sweden)

    Gabi Reinmann

    2005-05-01

    Full Text Available Begrifflichkeiten schaffen Bewusstsein, haben Wirkungen auf Denken und Handeln und können Zielsetzungen von morgen beeinflussen. Der vorliegende Beitrag möchte der Frage nachgehen, welche Funktion der Begriff des Wissensmanagements in pädagogischen Kontexten hat und künftig haben wird, welche Chancen und Risiken davon ausgehen und in welchem Verhältnis er zum Begriff der Medienbildung steht oder stehen könnte.

  2. Armut und Gesundheit

    OpenAIRE

    Lampert, Thomas; Kroll, Lars Eric

    2010-01-01

    Menschen in Armut haben einen schlechteren allgemeinen Gesundheitszustand, zeigen häufiger gesundheitsriskanteres Verhalten und haben ein erhöhtes Risiko für bestimmte Erkrankungen. Weitere Zusammenhänge von Armut und Gesundheit, dargestellt mit Ergebnissen der Studie "Gesundheit in Deutschland aktuell" (GEDA), können Sie der aktuellen Ausgabe von GBE kompakt entnehmen.

  3. Gesundheit, Essen und Nachhaltigkeit

    DEFF Research Database (Denmark)

    Reisch, Lucia

    2012-01-01

    zwischen Gesundheit, Essen und Nachhaltigkeit geht der Auftrag der Ernährungsaufklärung heute thematisch über den der klassischen Aufklärung hinaus. Vor diesem Hintergrund beschäftigt sich der Beitrag mit den Fragen, wie heute eine „gesunde, nachhaltige Ernährung“ - und damit das Ziel einer gelingenden...

  4. Steuern und Governance

    Directory of Open Access Journals (Sweden)

    Eduard Müller

    2014-05-01

    Full Text Available ENGLISH: Taxation, in the modern state, has long been a mass phenomenon with an interdisciplinary outlook. On the macro level of the state, a new generation of administrative reforms has crystallized under the label “good public governance”. These reforms seek to resolve regulatory interdependence of state and non - state actors by way of cooperation and interaction. In parallel, on the micro level of businesses, “corporate governance” – voluntary compliance with legal and ethical standards – has become an increasingly important issue. With a view to tax law and tax collection, these developments open up new possibilities to raise tax compliance by means of consensual and cooperative instruments and, accordingly, address taxation as a mass phenomenon. DEUTSCH: Besteuerung ist im modernen Staat ein Massenphänomen und längst interdisziplinär ausgerichtet. Auf der Makro-Ebene des Staates hat sich unter dem Begriff Good Public Governance eine neue Generation von Staats- und Verwaltungsreformen herausgebildet, die Regelungsbeziehungen von staatlichen und nichtstaatlichen Akteuren durch Kooperationen und Interaktionen zu lösen versucht. Parallel dazu hat auf der Mikro-Ebene der Unternehmen mit dem Thema Corporate Governance die freiwillige Einhaltung von rechtlichen und ethischen Regeln an Bedeutung gewonnen. Für das Steuerrecht und den Steuervollzug resultieren aus diesen Entwicklungen neue Möglichkeiten, durch Nutzung konsens- und kooperationsorientierter Instrumente die Tax Compliance zu erhöhen und so dem Massenphänomen Besteuerung gerecht zu werden.

  5. Neugier und epistemisches Handeln

    OpenAIRE

    2008-01-01

    Spezifische epistemische Neugier ist ein motivationaler Zustand, der durch konkrete Fragen ausgelöst und durch Wissenserwerb befriedigt wird. In der vorliegenden Arbeit geht es um die Entstehungsbedingungen spezifischer epistemischer Neugier und die Charakteristika neugiermotivierten Handelns. In kritischer Auseinandersetzung mit vorhandenen Neugiertheorien wird ein integratives Modell entwickelt, dem zufolge Neugier auf der metakognitiven Erfahrung einer gesteigerten kognitiven Aktivität ber...

  6. Thermodynamik grundlagen und technische anwendungen

    CERN Document Server

    Baehr, Hans Dieter

    2009-01-01

    Für die aktualisierte 14. Auflage des bewährten Lehrbuchs der Technischen Thermodynamik wurde das Kapitel über Wärmekraftanlagen gründlich bearbeitet und durch einen Abschnitt zur Energiewandlung ergänzt: Die Energiebedarfsstruktur von Deutschland wird erläutert, und die möglichen Energiewandlungspfade werden diskutiert. Schwerpunkt des Buches ist die ausführliche und auch dem Anfänger verständliche Darstellung der Grundlagen der Thermodynamik mit der sorgfältigen Einführung der thermodynamischen Begriffe und den fundamentalen Bilanzgleichungen für Energie, Entropie und Exergie. Die thermodynamischen Eigenschaften reiner Fluide und fluider Gemische werden eingehend erläutert. Darauf aufbauend wird die Thermodynamik der Gemische und der chemischen Reaktionen entwickelt. Auch die thermodynamischen Aspekte wichtiger energie- und verfahrenstechnischer Anwendungen werden praxisnah behandelt: - Strömungs- und Arbeitsprozesse, - thermische Stofftrennverfahren, - Verbrennungsprozesse und Verbrennungsk...

  7. Formation, characterization and mathematical modeling of the aerobic granular sludge

    Energy Technology Data Exchange (ETDEWEB)

    Ni, Bing-Jie [Queensland Univ., Brisbane, QLD (Australia). Advanced Water Management Centre

    2013-07-01

    Reports on successful aerobic granulation of sludge in pilot-scale reactor for treatment of low-strength municipal wastewater and identifies the key factors responsible for this process. Develops comprehensive models for sludge granulation, microbial interactions and microbial products formation to provide insights into the dynamics of all the soluble and solid components in aerobic granular sludge system. Demonstrates accelerated start-up and optimization of the anaerobic ammonia oxidation process by seeding the reactor with aerobic granules. Aerobic granular sludge technology will play an important role as an innovative technology alternative to the present activated sludge process in industrial and municipal wastewater treatment in the near future. Intended to fill the gaps in the studies of aerobic granular sludge, this thesis comprehensively investigates the formation, characterization and mathematical modeling of aerobic granular sludge, through integrating the process engineering tools and advanced molecular microbiology. The research results of this thesis contributed significantly to the advance of understanding and optimization of the bacterial granulation processes, the next generation of technology for cost-effective biological wastewater treatment.

  8. Community structure and denitrification ability of aerobic and anaerobic denitrifying bacteria from mangrove wetland%红树林湿地中好氧-厌氧反硝化菌脱氮特性及其种群结构分析

    Institute of Scientific and Technical Information of China (English)

    林娜; 郭楚玲; 郭延萍; 柯林; 党志; 谭凤仪

    2012-01-01

    Mangrove wetlands dominate the coastline of tropical and subtropical regions. With the expansion of human activity along riverine and coastal shorelines, mangroves are subject to anthropogenic inputs, which introduee additional nitrogen to mangrove ecosystems. In the present study, both traditional cultivation and molecular method ( clone library and RFLP) were used to investigate the community strueture and abilities of denitrifying microorganism from Mai Po mangrove sediment. Twenty aerobic and anaerobic denitrifying bacteria strains were obtained and most of them had the abilities to remove 98% NO3- -N in 2 days. The aerobic denitrifying bacterial strains belonged to the genera of Pseudomonas, Comamonas and Acinetobacter. The anaerobie denitrifying bacterial strains were Pseudomonas, Agrobacterium, Uncultured Betaproteobacteria bacterium. To investigate the diversity of denitrifying bacteria in the mangrove sediments, nosZ gene clone library were constructed. 26 out of 50 clones belonged to 11 unknown groups, while the others were the Pennisetum (26%) , the β-proteobacterium (10%) , the Entandrophragma ( 4 % ) , the Pseudomonas ( 4 % ) and the denitrifying bacterium (4%). This indicated that Mai Po mangrove had high degree of taxonomic diversity of denitrifiers.%随着沿岸水体氮素富营养化的加剧,生物脱氮作用越来越受重视,位于海陆交界的湿地红树林生态系统作为一个自然脱氮体系备受关注.本研究以典型亚热带湿地红树林(香港Mai Po)作为对象,结合传统的富集筛选和分子生物学方法--建立nosZ基因克隆文库和RFLP分析技术对红树林沉积物中反硝化细菌脱氮能力、种群结构和丰度进行研究.从Mai Po红树林沉积物中共筛选到12株好氧反硝化菌和8株厌氧反硝化菌,其中好氧反硝化菌包括Pseudomonas(4株)、Comamonas(2株)和Acinetobacter(2株)等7个菌属,厌

  9. Antepartuales fetales Elektrokardiogramm und Kardiotokographie

    OpenAIRE

    2006-01-01

    Es wurden 65 Patientinnen zwischen der 17. und 41. Schwangerschaftswoche unter Berücksichtigung der Hauptpathologien "intauterine Wachstumsretardierung" und "Gestationsdiabetes" simultan mit Hilfe der Kineto-Kardiotokographie (KCTG) und des fetalen abdominalen EKGs (AECG) untersucht. Neben dem direkten Vergleich von KCTG- und AECG-Messung wurde die Registrierqualität des AECG hinsichtlich verschiedener Einflussgrößen untersucht und eine Analyse der errechneten Durchschnitts-EKGs vorgenommen. ...

  10. Die amtliche Kinder- und Jugendhilfestatistik

    OpenAIRE

    Schilling, Matthias

    2003-01-01

    Unter Rückgriff auf verschiedene wissenschaftliche Methoden umfasst die Dissertation eine differenzierte Untersuchung der amtlichen Kinder- und Jugendhilfestatistik (KJH-Statistik). Da die wissenschaftliche Sozialpädagogik erst langsam und zögerlich beginnt, die amtliche Statistik der Kinder- und Jugendhilfe wahrzunehmen, liegt das Forschungsinteresse darin, dass eine grundlegende und umfassende wissenschaftliche Analyse der Möglichkeiten und Grenzen der KJH-Statistik vorgenommen wird. Die KJ...

  11. The Twin Arginine Translocation System Is Essential for Aerobic Growth and Full Virulence of Burkholderia thailandensis

    OpenAIRE

    Wagley, Sariqa; Hemsley, Claudia; Thomas, Rachael; Madeleine G Moule; Vanaporn, Muthita; Andreae, Clio; Robinson, Matthew; Goldman, Stan; Brendan W. Wren; Butler, Clive S.; Richard W Titball

    2014-01-01

    The twin arginine translocation (Tat) system in bacteria is responsible for transporting folded proteins across the cytoplasmic membrane, and in some bacteria, Tat-exported substrates have been linked to virulence. We report here that the Tat machinery is present in Burkholderia pseudomallei, B. mallei, and B. thailandensis, and we show that the system is essential for aerobic but not anaerobic growth. Switching off of the Tat system in B. thailandensis grown anaerobically resulted in filamen...

  12. 厌氧-好氧组合工艺处理垃圾渗滤液短程硝化的二次启动%THE SECOND STARTUP OF THE SHORT-CUT NITRIFICATION OF LANDFILL LEACHATE BY THE ANAEROBIC-AEROBIC PROCESS

    Institute of Scientific and Technical Information of China (English)

    刘牡; 彭永臻; 宋燕杰; 吴莉娜; 张靖倩

    2011-01-01

    A system consisting of a two-stage UASB and anoxic-oxic reactor was used to enhance COD and nitrogen removal fiom landfill leachate,focusing on methods and influencing factors of the second startup of the reactor.The results showed that: the SS, VSS, VSS / SS, settlement rate and the average particle size of the granular sludge in the two UASB rise periodically and the activity was resumed in a relatively short term through gradually increasing the organic loading and creating conducive conditions for anaerobic digestion such as temperature, pH, alkalinity, VFA and so on.NO2-N accumulation rate from the initial 19.4% increases to 90% in A/O system and remains stable with FA as the main controlling factor, and creating an appropriate temperature, pH, alkalinity, DO, and other conditions.By means of creating conditions to make the anaerobic and aerobic biological systems reach coordination and complementarity the reactor startup is completed in 6 weeks.COD and nitrogen was removed simultaneously and effectively by the biological system, the average removal efficiency of NH4+-N, TN and COD are up to 99%, 87%, 92%, respectively.When the average influent NH4+-N, TN concentrations and COD were 2 315 mg·L-1, 2 422 mg·L-1 and 12 800 mg·L-1%采用"两级UASB-A/O"组合工艺处理实际高氨氮城市生活垃圾渗滤液,对反应器二次启动的方法和影响因素进行了分析与考察.试验结果表明,通过逐步提高两级UASB的有机负荷,并创造有利于厌氧消化的温度、pH、碱度和VFA等条件,在较短的时间内使得两级UASB内颗粒污泥的SS、VSS、VSS/SS、沉降速率和平均粒径呈阶段性增加,生物活性得到迅速恢复;以FA为主要控制因素,创造适宜的温度,pH、碱度和DO等其它条件,并辅以过程控制,使得A/O系统中的NO2--N累积率从启动初期的19.4%上升到90%的短程硝化并得以稳定维持.兼顾两类生化系统使其达到相互协调而且优势互补的状态,6

  13. Combined elimination of organic C, sulfate and heavy metals. Final report; Kombinierte organische C-, Sulfat- und Schwermetalleliminierung. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Raebiger, N.

    2002-01-11

    A new biological process for purification of highly burdened industrial effluents was developed in which aerobic, oxygen-limiting reaction conditions ensure simultaneous elimination of organic C, sulfate and heavy metals in a single process step in a compact system. The design data are presented here for the purpose of practical implementation of the process. [German] Das Ziel des Forschungsvorhabens ist die Entwicklung eines neuen biologischen Verfahrens zur Reinigung hochbelasteter Industrieabwaesser, bei dem durch die Einstellung aerober, sauerstofflimitierender Reaktionsbedingungen eine kombinierte organisch C-, Sulfat- und Schwermetalleliminierung gleichzeitig in einem Verfahrensschritt und kompakter Anlagentechnik realisiert wird. Hierbei werden die Auslegungsunterlagen fuer die praxisrelevante Umsetzung dieses Verfahrens zur Verfuegung gestellt. (orig.)

  14. Ultrasound and ozone assisted biological degradation of thermally pretreated and anaerobically pretreated distillery wastewater.

    Science.gov (United States)

    Sangave, Preeti C; Gogate, Parag R; Pandit, Aniruddha B

    2007-05-01

    The present work is aimed at increasing the overall efficiency of the treatment process of distillery spent wash using a combination of different treatment techniques. Initially the effluent samples were subjected to Thermal Pretreatment (TPT-DW) and anaerobic treatment (ANA-DW). Advanced oxidation techniques, viz., Ultrasound (US) and Ozone were then used for further COD reduction followed by the conventional aerobic oxidation using mixed microbial consortium. Pretreatment of TPT-DW with US and Ozone (as stand alone techniques) enhanced the subsequent aerobic oxidation rate. For US treated sample, a maximum of 13% COD reduction was attained at the end of 48 h of aerobic oxidation, while for the ozone treated effluent a maximum of 45.6% COD reduction was obtained as compared to mere 1.8% COD reduction for the control (TPT-DW directly subjected to aerobic oxidation) indicating a 25 times increase in the rate of aerobic biodegradation of ozonated sample. Anaerobically treated effluent sample (ANA-DW) could be successfully treated aerobically. In this case, however, the use of advanced oxidation techniques did not result in any synergistic effects. The rate of the aerobic oxidation was slightly higher for the control (ANA-DW directly subjected to aerobic oxidation) as compared to the sample pretreated using ultrasound or ozonation. TOC analysis revealed that between the two pretreatments studied, ozone was found to be superior over US as it led to both COD as well as TOC reduction during the aerobic oxidation step for ANA-DW effluent stream.

  15. Multiple Sklerose und Schwangerschaft

    Directory of Open Access Journals (Sweden)

    Berger T

    2009-01-01

    Full Text Available Da Multiple Sklerose (MS eine der häufigsten neurologischen Erkrankungen im jungen Erwachsenenalter ist und Frauen zwei- bis dreimal häufiger als Männer betrifft, sind Fragen nach Fertilität, Schwangerschaft und Geburt ein bedeutsames Gesprächsthema zwischen Patientinnen und behandelndem Arzt. Eine Schwangerschaft geht einher mit multiplen physiologischen Veränderungen, die auch zu einer Änderung des Immunstatus führen und so den Verlauf der MS (günstig beeinflussen können. Für die Dauer der Schwangerschaft ist eine Stabilisierung der MS mit einer Abnahme der Schubfrequenz festzustellen. Obwohl es dann in den ersten 6 Monaten postpartal zu einer Zunahme der Krankheitsaktivität kommen kann, lässt sich grundsätzlich sagen, dass eine Schwangerschaft keine längerfristigen negativen Effekte auf den weiteren Krankheitsverlauf ausübt. Auch hat MS keinen nachteiligen Einfluss auf die fetale Entwicklung und den positiven Geburtsausgang. Prinzipiell sind alle immunmodulatorischen und immunsuppressiven Medikamente in der Schwangerschaft kontraindiziert und sollten daher bei geplanter oder eingetretener Schwangerschaft unverzüglich abgesetzt werden. Eine Schubtherapie mit Kortikosteroiden kann hingegen durchaus auch während einer bestehenden Schwangerschaft durchgeführt werden.

  16. Mediendidaktik und Wissensmanagement

    Directory of Open Access Journals (Sweden)

    Gabi Reinmann-Rothmeier

    2002-10-01

    Full Text Available Mediendidaktik und Wissensmanagement – das ist auf den ersten Blick ein ungleiches Paar: Die Mediendidaktik ist eine Teildisziplin der (Medien- Pädagogik, während Wissensmanagement ein genuin wirtschaftliches Thema mit betriebswirtschaftlichen Akzenten ist. Annäherungen zwischen der Mediendidaktik und Wissensmanagement gibt es zum einen durch den sog. E-Learning-Trend seitens der Wirtschaft, der mediendidaktisches Wissen und Können auf den Plan ruft, zum anderen durch wachsendes Interesse am Thema Wissensmanagement seitens der Pädagogik. Der Beitrag beschreibt sowohl für das E-Learning als auch für das Wissensmanagement aus pädagogisch-psychologischer Sicht jeweils ein Orientierungsmodell; beide Modelle machen die Berührungspunkte zwischen Mediendidaktik und Wissensmanagement deutlich. Neben einem Überblick über wirtschaftliche Argumente für eine Verschmelzung von E-Learning und Wissensmanagement werden aufbauend auf den beiden Orientierungsmodellen theoretische und praktische Verbindungslinien zwischen E-Learning und Wissensmanagement sowie die Rolle der Mediendidaktik in dem daraus entstehenden Wechselverhältnis herausgearbeitet.

  17. Teilchendetektoren Grundlagen und Anwendungen

    CERN Document Server

    Kolanoski, Hermann

    2016-01-01

    In diesem Buch werden die experimentellen Grundlagen von Teilchendetektoren und ihre Anwendung in Experimenten beschrieben. Die Entwicklung von Detektoren ist ein wichtiger Bestandteil der Teilchen-, Astroteilchen- und Kernphysik und gehört daher zum Handwerk des Experimentalphysikers in diesen Gebieten. Dieses umfassende Werk beinhaltet den kompletten Stoff für entsprechende Master-Module in der experimentellen Teilchenphysik, geht aber im Inhalt auch darüber hinaus. Zielgruppe sind Studierende, die sich in die Materie vertiefen möchten, aber auch Lehrende und Wissenschaftler, die das Buch zum Einstieg in das wissenschaftliche Arbeiten an Detektorentwicklungen verwenden können. Zielrichtung des Buches ist, die physikalischen Grundlagen für die Detektoren und ihrer verschiedenen Ausführungen so klar wie möglich und so tiefgehend wie nötig darzustellen. Die Breite des für die Detektorentwicklung nötigen Wissens umfasst viele Bereiche der Physik und Technik, von den Wechselwirkun...

  18. Integrated sequential anaerobic/aerobic biodegradation of azo dyes

    NARCIS (Netherlands)

    Tan, N.C.G.

    2001-01-01

    Azo dyes constitute a major class of environmental pollutants accounting for 60 to 70% of all dyes and pigments used. These compounds are characterized by aromatic moieties linked together with azo groups (-N=N-). The release of azo dyes into the environment is a concern due to coloration of natural

  19. Thermophilic aerobic post treatment of anaerobically pretreated paper process water

    OpenAIRE

    Vogelaar, J.C.T.

    2002-01-01

    Thermophilic waste- or process water treatment increases in importance as industries shift from end-of-pipe treatment towards integrated process water treatment. The need for process water treatment becomes evident as the levels of pollutants in industrial water circuits need to be controlled whereas the intake of fresh water generally diminishes. In the paper and board industry, high process water temperatures prevail and thus water treatment needs to take place under thermophilic conditions...

  20. Thermophilic aerobic post treatment of anaerobically pretreated paper process water

    NARCIS (Netherlands)

    Vogelaar, J.C.T.

    2002-01-01

    Thermophilic waste- or process water treatment increases in importance as industries shift from end-of-pipe treatment towards integrated process water treatment. The need for process water treatment becomes evident as the levels of pollutants in industrial water circuits need to be co

  1. Kinder- und Jugendliteratur – ein literarisches Schattendasein

    Directory of Open Access Journals (Sweden)

    Gisela Bürki

    2002-07-01

    Full Text Available Das zweibändige Taschenbuch der Kinder- und Jugendliteratur behandelt in 43 von verschiedenen Autorinnen und Autoren verfassten Beiträgen sowohl Begriffsbestimmung und Geschichte der deutschsprachigen Kinder- und Jugendliteratur als auch gattungstheoretische Fragen, Thematisches ausgewählte thematische Aspekte und verwandte Medien. Das Handbuch dürfte sich als ein Basiswerk der Kinder- und Jugendliteraturforschung herausstellen.

  2. Concerning the role of cell lysis-cryptic growth in anaerobic side-stream reactors: the single-cell analysis of viable, dead and lysed bacteria.

    Science.gov (United States)

    Foladori, P; Velho, V F; Costa, R H R; Bruni, L; Quaranta, A; Andreottola, G

    2015-05-01

    In the Anaerobic Side-Stream Reactor (ASSR), part of the return sludge undergoes alternating aerobic and anaerobic conditions with the aim of reducing sludge production. In this paper, viability, enzymatic activity, death and lysis of bacterial cells exposed to aerobic and anaerobic conditions for 16 d were investigated at single-cell level by flow cytometry, with the objective of contributing to the understanding of the mechanisms of sludge reduction in the ASSR systems. Results indicated that total and viable bacteria did not decrease during the anaerobic phase, indicating that anaerobiosis at ambient temperature does not produce a significant cell lysis. Bacteria decay and lysis occurred principally under aerobic conditions. The aerobic decay rate of total bacteria (bTB) was considered as the rate of generation of lysed bacteria. Values of bTB of 0.07-0.11 d(-1) were measured in anaerobic + aerobic sequence. The enzymatic activity was not particularly affected by the transition from anaerobiosis to aerobiosis. Large solubilisation of COD and NH4(+) was observed only under anaerobic conditions, as a consequence of hydrolysis of organic matter, but not due to cell lysis. The observations supported the proposal of two independent mechanisms contributing equally to sludge reduction: (1) under anaerobic conditions: sludge hydrolysis of non-bacterial material, (2) under aerobic conditions: bacterial cell lysis and oxidation of released biodegradable compounds.

  3. RESPONSE OF RICE ROOT IN RESPIRATION AT JOINTING STAGE TO OZONE POLLUTION AND ALTERNATION OF ANAEROBIC AND AEROBIC CONDITIONS%水稻拔节期根系呼吸对臭氧污染和厌氧—有氧环境变化的响应

    Institute of Scientific and Technical Information of China (English)

    寇太记; 朱建国

    2013-01-01

    Effects of elevated atmospheric ozone (pO3) (50% higher than the ambient pO3 in concentration) on root respiration and biomass accumulation and distribution of rice (Oryza sativa L.cv.Xiandao 63) at the jointing stage were investigated in fully open-air field conditions,and effect of alternation of anaerobic and aerobic conditions on root respiration was analyzed using special gas-collecting installations.Results show that under elevated pO3canopy and total biomass of the crop decreased slightly,while its root dry matter weight and ratio of root/canopy dropped significantly by 14.7% and 10.4%,respectively.The mixture of N2 and O2at a ratio of 9∶1 or 9.5∶0.5 was the most propitious for root respiration,whereas in pure N2,natural air and CO2-saturated distilled water root respiration rate was lowered to a varying degree.The treatment of elevated pO3 coupled with measurement inn CO2-saturated distilled water and the treatment of ambient air coupled with measurement in pure N2 was the least in root respiration rate,indicating that although the condition in which root respiration was measured affected root respiration rate,the impact was restricted in degree by the atmospheric environment in which the plant grew.The root respiration rate of rice growing under elevated ozone was 23.6% ~52.7% higher than that under natural atmosphere when measured in pure gas condition,and the difference between the two was insignificant when the measurement was done in CO2-saturated distilled water,showing that the influence of ozone pollution on root respiration obviously decreased.Under elevated and ambient pO3 environments,root respiration of the rice displayed a curve of convex quadratic function with increasing oxygen supply in the gaseous environment for measurement.The oxygen concentration of 5%~ 10% in the environment promoted rice root respiration,while stronge anaerobic condition (i.e.,pure N2) and aerobic conditions (i.e.,air) both affected root

  4. Anaerobic Digestion: Process

    DEFF Research Database (Denmark)

    Angelidaki, Irini; Batstone, Damien J.

    2011-01-01

    with very little dry matter may also be called a digest. The digest should not be termed compost unless it specifically has been composted in an aerated step. This chapter describes the basic processes of anaerobic digestion. Chapter 9.5 describes the anaerobic treatment technologies, and Chapter 9.......6 addresses the mass balances and environmental aspects of anaerobic digestion....

  5. Tar water digestion in an upflow anaerobic sludge blanket reactor

    Energy Technology Data Exchange (ETDEWEB)

    Skibsted Mogensen, A.; Angelidaki, I.; Schmidt, J.E.; Ahring, B.K. [Technical Univ., Dept. of Environmental Science and Engineering, Lyngby (Denmark)

    1998-08-01

    The water from the gasification and wet oxidised tar water has been digested anaerobically in UASB reactors and were digested in respectively 10 and 50% in batches. Though the tar water show inhibition at very low concentrations to aerobic microorganisms, the granular sludge used in UASB reactors degrades tar water in concentrations that reveal total inhibition of e.g. bacteria conducting the nitrification process. The value of waste waters are determined, showing that the tar water produces more biogas in the anaerobic digestion. A wide range of xenobiotics, especially phenolic compounds can be transformed in the anaerobic digestion process. Seven phenolic are followed in batch experiments and UASB reactor experiments, and their particular fate in the anaerobic systems embody large differences in the transformation pattern. (au) 24 refs.

  6. The interplay between aerobic metabolism and antipredator performance: vigilance is related to recovery rate after exercise

    Directory of Open Access Journals (Sweden)

    Shaun Steven Killen

    2015-04-01

    Full Text Available When attacked by a predator, fish respond with a sudden fast-start motion away from the threat. Although this anaerobically-powered swimming necessitates a recovery phase which is fuelled aerobically, little is known about links between escape performance and aerobic traits such as aerobic scope or recovery time after exhaustive exercise. Slower recovery ability or a reduced aerobic scope could make some individuals less likely to engage in a fast-start response or display reduced performance. Conversely, increased vigilance in some individuals could permit faster responses to an attack but also increase energy demand and prolong recovery after anaerobic exercise. We examined how aerobic scope and the ability to recover from anaerobic exercise relates to differences in fast-start escape performance in juvenile golden grey mullet at different acclimation temperatures. Individuals were acclimated to either 18, 22, or 26oC, then measured for standard and maximal metabolic rates and aerobic scope using intermittent flow respirometry. Anaerobic capacity and the time taken to recover after exercise were also assessed. Each fish was also filmed during a simulated attack to determine response latency, maximum speed and acceleration, and turning rate displayed during the escape response. Across temperatures, individuals with shorter response latencies during a simulated attack are those with the longest recovery time after exhaustive anaerobic exercise. Because a short response latency implies high preparedness to escape, these results highlight the trade-off between the increased vigilance and metabolic demand, which leads to longer recovery times in fast reactors. These results improve our understanding of the intrinsic physiological traits that generate inter-individual variability in escape ability, and emphasise that a full appreciation of trade-offs associated with predator avoidance and energy balance must include energetic costs associated with

  7. Fertilization stimulates anaerobic fuel degradation of antarctic soils by denitrifying microorganisms.

    Science.gov (United States)

    Powell, Shane M; Ferguson, Susan H; Snape, Ian; Siciliano, Steven D

    2006-03-15

    Human activities in the Antarctic have resulted in hydrocarbon contamination of these fragile polar soils. Bioremediation is one of the options for remediation of these sites. However, little is known about anaerobic hydrocarbon degradation in polar soils and the influence of bioremediation practices on these processes. Using a field trial at Old Casey Station, Antarctica, we assessed the influence of fertilization on the anaerobic degradation of a 20-year old fuel spill. Fertilization increased hydrocarbon degradation in both anaerobic and aerobic soils when compared to controls, but was of most benefit for anaerobic soils where evaporation was negligible. This increased biodegradation in the anaerobic soils corresponded with a shift in the denitrifier community composition and an increased abundance of denitrifiers and benzoyl-CoA reductase. A microcosm study using toluene and hexadecane confirmed the degradative capacity within these soils under anaerobic conditions. It was observed that fertilized anaerobic soil degraded more of this hydrocarbon spike when incubated anaerobically than when incubated aerobically. We conclude that denitrifiers are actively involved in hydrocarbon degradation in Antarctic soils and that fertilization is an effective means of stimulating their activity. Further, when communities stimulated to degrade hydrocarbons under anaerobic conditions are exposed to oxygen, hydrocarbon degradation is suppressed. The commonly accepted belief that remediation of polar soils requires aeration needs to be reevaluated in light of this new data.

  8. Assessment of aerobic and respiratory growth in the Lactobacillus casei group.

    Directory of Open Access Journals (Sweden)

    Teresa Zotta

    Full Text Available One hundred eighty four strains belonging to the species Lactobacillus casei, L. paracasei and L. rhamnosus were screened for their ability to grow under aerobic conditions, in media containing heme and menaquinone and/or compounds generating reactive oxygen species (ROS, in order to identify respiratory and oxygen-tolerant phenotypes. Most strains were able to cope with aerobic conditions and for many strains aerobic growth and heme or heme/menaquinone supplementation increased biomass production compared to anaerobic cultivation. Only four L. casei strains showed a catalase-like activity under anaerobic, aerobic and respiratory conditions and were able to survive in presence of H2O2 (1 mM. Almost all L. casei and L. paracasei strains tolerated menadione (0.2 mM and most tolerated pyrogallol (50 mM, while L. rhamnosus was usually resistant only to the latter compound. This is the first study in which an extensive screening of oxygen and oxidative stress tolerance of members of the L. casei group has been carried out. Results allowed the selection of strains showing the typical traits of aerobic and respiratory metabolism (increased pH and biomass under aerobic or respiratory conditions and unique oxidative stress response properties. Aerobic growth and respiration may confer technological and physiological advantages in the L. casei group and oxygen-tolerant phenotypes could be exploited in several food industry applications.

  9. Aerobic conditions increase isoprenoid biosynthesis pathway gene expression levels for carotenoid production in Enterococcus gilvus.

    Science.gov (United States)

    Hagi, Tatsuro; Kobayashi, Miho; Nomura, Masaru

    2015-06-01

    Some lactic acid bacteria that harbour carotenoid biosynthesis genes (crtNM) can produce carotenoids. Although aerobic conditions can increase carotenoid production and crtNM expression levels, their effects on the pathways that synthesize carotenoid precursors such as mevalonate and isoprene are not completely understood. In this study, we investigated whether aerobic conditions affected gene expression levels involved in the isoprenoid biosynthesis pathway that includes the mevalonate and isoprene biosynthesis pathways in Enterococcus gilvus using real-time quantitative reverse transcription PCR. NADH oxidase (nox) and superoxide dismutase (sod) gene expression levels were investigated as controls for aerobic conditions. The expression levels of nox and sod under aerobic conditions were 7.2- and 8.0-fold higher, respectively, than those under anaerobic conditions. Aerobic conditions concomitantly increased the expression levels of crtNM carotenoid biosynthesis genes. HMG-CoA synthase gene expression levels in the mevalonate pathway were only slightly increased under aerobic conditions, whereas the expression levels of HMG-CoA reductase and five other genes in the isoprene biosynthesis pathways were 1.2-2.3-fold higher than those under anaerobic conditions. These results demonstrated that aerobic conditions could increase the expression levels of genes involved in the isoprenoid biosynthesis pathway via mevalonate in E. gilvus.

  10. Contribution of anaerobic energy expenditure to whole body thermogenesis

    Directory of Open Access Journals (Sweden)

    Scott Christopher B

    2005-06-01

    Full Text Available Abstract Heat production serves as the standard measurement for the determination of energy expenditure and efficiency in animals. Estimations of metabolic heat production have traditionally focused on gas exchange (oxygen uptake and carbon dioxide production although direct heat measurements may include an anaerobic component particularly when carbohydrate is oxidized. Stoichiometric interpretations of the ratio of carbon dioxide production to oxygen uptake suggest that both anaerobic and aerobic heat production and, by inference, all energy expenditure – can be accounted for with a measurement of oxygen uptake as 21.1 kJ per liter of oxygen. This manuscript incorporates contemporary bioenergetic interpretations of anaerobic and aerobic ATP turnover to promote the independence of these disparate types of metabolic energy transfer: each has different reactants and products, uses dissimilar enzymes, involves different types of biochemical reactions, takes place in separate cellular compartments, exploits different types of gradients and ultimately each operates with distinct efficiency. The 21.1 kJ per liter of oxygen for carbohydrate oxidation includes a small anaerobic heat component as part of anaerobic energy transfer. Faster rates of ATP turnover that exceed mitochondrial respiration and that are supported by rapid glycolytic phosphorylation with lactate production result in heat production that is independent of oxygen uptake. Simultaneous direct and indirect calorimetry has revealed that this anaerobic heat does not disappear when lactate is later oxidized and so oxygen uptake does not adequately measure anaerobic efficiency or energy expenditure (as was suggested by the "oxygen debt" hypothesis. An estimate of anaerobic energy transfer supplements the measurement of oxygen uptake and may improve the interpretation of whole-body energy expenditure.

  11. Improvement of methane generation capacity by aerobic pre-treatment of organic waste with a cellulolytic Trichoderma viride culture.

    Science.gov (United States)

    Wagner, Andreas Otto; Schwarzenauer, Thomas; Illmer, Paul

    2013-11-15

    Trichoderma viride is known as a potent cellulose decomposer and was successfully used to improve and accelerate the decomposition process of aerobic composting. In contrast, the role of fungi as pre-treatment organisms for anaerobic digestion is not clear, since the fast aerobic decomposition is thought to be responsible for a rapid depletion of easily available nutrients, leading to a lack of these for the anaerobic community. In the present study carried out in lab-scale, the application of T. viride for the aerobic pre-incubation of organic matter derived from the inlet port of a 750,000 L anaerobic digester led to an increase in total gas and methane production in a subsequent anaerobic digestion step. A high cellulase activity caused by the addition of T. viride seemed to be responsible for a better nutrient availability for anaerobic microorganisms. Therefore, aerobic pre-incubation of organic residues with T. viride for subsequent anaerobic digestion is a promising approach in order to increase methane yields.

  12. Onkologie und Nervensystem

    Directory of Open Access Journals (Sweden)

    Grisold W

    2009-01-01

    Full Text Available Die Zusammenhänge zwischen dem Nervensystem und onkologischen Erkrankungen beschränken sich nicht nur auf primäre Hirntumoren, sondern gewinnen bei zahlreichen Tumorerkrankungen, einerseits aufgrund der verbesserten Überlebenszeit, andererseits aufgrund von therapiebedingter Neurotoxizität, an Bedeutung. Es handelt sich um direkte Tumoreinwirkungen wie Metastasen oder meningeale Ausbreitungen, Auswirkung der Therapie im Sinne von Neurotoxizität, metabolischen Phänomenen, Infektionen und paraneoplastische Syndrome. Besondere Bedeutung verdient die Kontrolle von symptomatischen Anfällen und die Behandlung von Schmerzen bei onkologischen Patienten. Supportive und palliative Maßnahmen gewinnen zwar zunehmend an Bedeutung, sind aber in der Neurologie noch weitgehend in Entwicklung. Vertieftes Wissen über neurologische Störungen bei onkologischen Patienten im Sinne einer neuroonkologischen Qualifikation ist anzustreben.

  13. Niere und Hypertonie

    Directory of Open Access Journals (Sweden)

    Watschinger B

    2004-01-01

    Full Text Available Eine Hypertonie kann Ursache, aber auch Folge einer Nierenerkrankung sein. Der Blutdruck trägt zur Progression der renalen Erkrankung und zum exzessiv hohen kardiovaskulären Risiko der Patienten bei. Der Zielblutdruck bei Patienten mit einer eingeschränkten Nierenfunktion oder gleichzeitig bestehender Proteinurie unter 1 g/Tag liegt bei 130/80 mmHg, bei einer Proteinurie über 1 g/Tag 125/75 mmHg und bei Patienten mit diabetischer Nephropathie 130/80 mm Hg. ACE-Hemmer oder ARB sind Mittel der 1. Wahl, da sie stärker als andere Substanzen antiproteinurisch wirken. Um die Zielblutdruckwerte zu erreichen, ist jedoch meist eine antihypertensive Kombinationstherapie (evt. mit Diuretika und/oder Nicht-Dihydropyridin-Kalziumantagonisten, bzw. Kombination von ACE-Hemmer und ARB erforderlich.

  14. Therapieeskalation: Wann und wie?

    Directory of Open Access Journals (Sweden)

    Lutterotti A

    2010-01-01

    Full Text Available Für Patienten mit Multipler Sklerose, für die mit einer immunmodulierenden Basistherapie keine ausreichende Verminderung der Krankheitsaktivität erreicht werden kann, ist eine Eskalationstherapie vorgesehen. Man geht davon aus, dass bei diesen Patienten eine frühe und effektive Intensivierung der Immuntherapie die entzündliche Krankheitsaktivität hemmen kann und die langfristige Prognose günstig beeinflusst. Es stehen verschiedene monoklonale Antikörper und immunsuppressive Substanzen zur Verfügung, allerdings gibt es nur zum Teil gute Evidenz aus kontrollierten Studien. In diesem Übersichtsartikel gehen wir auf die Rationale für den Beginn einer Eskalationstherapie ein und diskutieren die unterschiedlichen Substanzen.

  15. Wasserball und Kommunismus

    NARCIS (Netherlands)

    Celikates, R.

    2010-01-01

    Zuletzt recht medienwirksam verbreiten Philosophen wie Slavoj Žižek und Alain Badiou eine vage «kommunistische Hypothese» - wie sehen die Theoriealternativen zu dieser neokommunistischen Intervention aus, die zugleich eine Abkehr vom Marxismus ist?

  16. Supraleitung Grundlagen und Anwendungen

    CERN Document Server

    Buckel, Werner

    2004-01-01

    Seit nunmehr drei Jahrzehnten ist der "Buckel" das Referenzwerk in deutscher Sprache zum Thema Supraleitung. Immer wieder aktualisiert, hat das Lehrbuch Generationen von Studenten begleitet. In der 6., vollständig überarbeiteten Auflage wird das bewährte didaktische Konzept beibehalten, inhaltlich wurde der Band dem aktuellen Stand von Forschung und Technik angepasst. Das Autorenteam um Prof. Kleiner verzichtet auf komplizierte mathematische Herleitungen. Es baut ganz auf das bewährte Step-by-Step-Prinzip: Der Schwierigkeitsgrad wird von Kapitel zu Kapitel gesteigert. So können auch Einsteiger ohne einschlägige Vorkenntnisse dem Stoff bequem folgen. Dank seiner klaren Sprache und zahlreicher Abbildungen eignet sich der Band hervorragend als einführendes Lehrbuch - und zwar nicht nur für Physik-Studenten, sondern auch für Studierende angrenzender Natur- und Ingenieurwissenschaften. Grundlegende Eigenschaften, supraleitende Elemente, Cooper-Paarung, Thermodynamik, kritische Ströme, Josephsonkontakte...

  17. Hypertonie und Adipositas

    Directory of Open Access Journals (Sweden)

    Abrahamian H

    2001-01-01

    Full Text Available Adipositas ist eine komplexe, multifaktorielle Erkrankung, die häufig mit Hypertonie assoziiert ist. Daten aus der Framingham-Studie zeigen, daß bei 78 % der Männer und bei 65 % der Frauen mit essentieller Hypertonie die Adipositas als ursächlich dafür betrachtet werden kann. Die dafür verantwortlichen pathophysiologischen Mechanismen sind ungenügend aufgeklärt und daher vielfach noch spekulativ. Die Hypothese, daß die Insulinresistenz als hauptverantwortlicher Faktor für die Entstehung der Hypertonie bei adipösen Patienten zu betrachten ist, wird in zahlreichen Studien durch signifikante Korrelation zwischen Insulinspiegel und Hypertonie belegt. Jedoch hat sich mehrfach gezeigt, daß diese Korrelationen mit hoher Wahrscheinlichkeit speziesabhängig sind. Ob nun bei Menschen ein kausaler Zusammenhang zwischen Hypertonie und Insulinresistenz angenommen werden kann, bleibt hypothetisch. Die Kausalität dieser engen Assoziation könnte in 4 Mechanismen begründet sein: Natrium-Retention, Hyperaktivität des sympathischen Nervensystems, gestörter membranöser Ionen-Transport und Proliferation von glatten Gefäß-Muskelzellen. In einem Vergleich zwischen hypertensiven und normotensiven alters- und gewichtsgematchten Probanden zeigte sich, daß hypertensive Patienten generell einen erhöhten Insulin-Response nach Glukose-Belastung aufwiesen. Dieser Effekt konnte unabhängig vom Körpergewicht nachgewiesen werden. Eine weitere Untersuchung, die bei jungen, schlanken Hypertonikern eine deutliche Verringerung der Insulin-induzierten Glukose-Aufnahme in einem Clamp-Versuch findet, bestätigt dieses Ergebnis. Andere Erklärungsansätze für die Assoziation Adipositas und Hypertonie sind erhöhter Leptinspiegel, ein TNF-Alpha-Genlocus für Adipositas und Hypertonie und ein im Fettgewebe lokal wirksames RAS. Als therapeutische Intervention steht die Gewichtsreduktion an erster Stelle, wobei hier auch der Einsatz von Medikamenten wie Appetitz

  18. Anaerobic degradation of linear alkylbenzene sulfonate.

    Science.gov (United States)

    Mogensen, Anders S; Haagensen, Frank; Ahring, Birgitte K

    2003-04-01

    Linear alkylbenzene sulfonate (LAS) found in wastewater is removed in the wastewater treatment facilities by sorption and aerobic biodegradation. The anaerobic digestion of sewage sludge has not been shown to contribute to the removal. The concentration of LAS based on dry matter typically increases during anaerobic stabilization due to transformation of easily degradable organic matter. Hence, LAS is regarded as resistant to biodegradation under anaerobic conditions. We present data from a lab-scale semi-continuously stirred tank reactor (CSTR) spiked with linear dodecylbenzene sulfonate (C12 LAS), which show that C12 LAS was biodegradable under methanogenic conditions. Sorption of C12 LAS on sewage sludge was described with a Freundlich isotherm. The C12 LAS sorption was determined with different concentrations of total solids (TS). In the semi-continuously stirred tank reactor, 18% of the added C12 LAS was bioavailable and 20% was biotransformed when spiking with 100 mg/L of C12 LAS and a TS concentration of 14.2 mg/L. Enhanced bioavailability of C12 LAS was obtained in an upflow anaerobic sludge blanket (UASB) reactor inoculated with granular sludge and sewage sludge. Biodegradation under thermophilic conditions was 37% with LAS as sole carbon source. Benzaldehyde was produced in the UASB reactor during LAS transformation.

  19. Cultivation of anaerobic and facultatively anaerobic bacteria from spacecraft-associated clean rooms.

    Science.gov (United States)

    Stieglmeier, Michaela; Wirth, Reinhard; Kminek, Gerhard; Moissl-Eichinger, Christine

    2009-06-01

    In the course of this biodiversity study, the cultivable microbial community of European spacecraft-associated clean rooms and the Herschel Space Observatory located therein were analyzed during routine assembly operations. Here, we focused on microorganisms capable of growing without oxygen. Anaerobes play a significant role in planetary protection considerations since extraterrestrial environments like Mars probably do not provide enough oxygen for fully aerobic microbial growth. A broad assortment of anaerobic media was used in our cultivation strategies, which focused on microorganisms with special metabolic skills. The majority of the isolated strains grew on anaerobic, complex, nutrient-rich media. Autotrophic microorganisms or microbes capable of fixing nitrogen were also cultivated. A broad range of facultatively anaerobic bacteria was detected during this study and also, for the first time, some strictly anaerobic bacteria (Clostridium and Propionibacterium) were isolated from spacecraft-associated clean rooms. The multiassay cultivation approach was the basis for the detection of several bacteria that had not been cultivated from these special environments before and also led to the discovery of two novel microbial species of Pseudomonas and Paenibacillus.

  20. Soziale Faktoren und Arbeitssicherheit

    OpenAIRE

    Krause-Jüttler, Grit

    2011-01-01

    Die Dissertation setzt sich mit der Thematik der Arbeitssicherheit in produzierenden kleinen und mittelständischen Unternehmen der sächsischen Metall- und Feinmechanikindustrie auseinander. Dabei untersucht die Autorin mögliche Einflussfaktoren auf das Sicherheitsverhalten von Arbeitnehmer/innen, um Hinweise für verbesserte personalwirtschaftliche Methoden zu identifizieren. Dabei liegt der Schwerpunkt der Arbeit auf dem Einfluss, den direkte Kolleg/innen auf das individuelle Sicherheitsverha...

  1. Epilepsiechirurgie und Neurostimulation

    Directory of Open Access Journals (Sweden)

    Baumgartner C

    2009-01-01

    Full Text Available Zirka 35 % aller Epilepsiepatienten leiden an einer medikamentös therapieresistenten Epilepsie. Für diese Patienten stellen die Epilepsiechirurgie (als potenziell kurative Methode und Neurostimulationsverfahren (als palliative Verfahren wertvolle Behandlungsoptionen dar. Bei den epilepsiechirurgisch behandelbaren Epilepsiesyndromen lassen sich (1 Epilepsien mit fokalen Pathologien, bei denen eine umschriebene Resektion durchgeführt werden kann; (2 Epilepsien, die extensive, oft multilobäre Resektionen oder Hemisphärektomien erfordern und (3 Epilepsien, bei denen lediglich eine Diskonnektionsoperation sinnvoll ist, unterscheiden. Voraussetzung für einen epilepsiechirurgischen Eingriff ist eine exakte präoperative Epilepsiediagnostik, wobei die epileptogene Zone und essenzielle Hirnregionen genau lokalisiert werden müssen. Dadurch werden prognostische Aussagen bezüglich der postoperativen Anfallskontrolle und eine Abschätzung des Risikos funktioneller Defizite ermöglicht. Gemäß einer rezenten Metaanalyse ist der mediane Anteil der langfristig anfallsfreien Patienten nach Temporallappenresektionen 66 %, nach okzipitalen und parietalen Resektionen je 46 % und nach frontalen Resektionen 27 %. Die Vagus- Nerv-Stimulation (VNS ist eine klinisch etablierte Methode für Patienten, die für einen epilepsiechirurgischen Eingriff nicht infrage kommen oder bei denen eine solche Operation nicht zum Erfolg geführt hat. Mit der VNS können Responderraten (Anfallsreduktion 50 % von 30–40 % erzielt werden, wobei die Wirksamkeit über die ersten 18–24 Monate kontinuierlich zunimmt, um dann ein stabiles Plateau zu erreichen. Bei der direkten Hirnstimulation, die sich derzeit noch im experimentellen Stadium befindet, kann einerseits eine direkte Stimulation der epileptogenen Zone und somit kortikaler Strukturen erfolgen, andererseits können auch tiefe Hirnstrukturen mit dem Ziel der Modifikation der Anfallsentstehung und/oder Ausbreitung stimuliert

  2. Sprichwort und Wortspiel

    Directory of Open Access Journals (Sweden)

    Željka Matulina

    1994-12-01

    Full Text Available Als eine idiomatische Bildung mit dem Satz-Status wird das Sprichwort meist aus der lexikographischen Diskussion ausgeschlossen, weil es Satz ist, auf der anderen Seite wird es im Rahmen der Syntax nicht behandelt, weil es Idiom ist, und so hat das Überprüfen und Testen seiner grammatischen Strukturiertheit wenig Sinn. Die Spezifik des Sprichwort-Satzes liegt in der linear-horizontalen Kombinatorik seiner Konstituenten. Die Reihenfolge der Sprichwort-Konstituenten ist streng festgelegt.

  3. Psychopharmaka und sexuelle Dysfunktion

    Directory of Open Access Journals (Sweden)

    Assem-Hilger E

    2005-01-01

    Full Text Available Moderne psychopharmakologische Therapieansätze reduzieren psychiatrische Zielsymptome und berücksichtigen in zunehmendem Maße prognostisch bedeutsame Faktoren wie subjektive Lebensqualität und soziale Reintegration. Im Rahmen der Complianceforschung wurden in den letzten Jahren sexuelle Nebenwirkungen von Psychopharmaka, insbesondere von antidepressiven und antipsychotischen Substanzen, vermehrt untersucht. Die komplexen Wechselwirkungen zwischen psychosozialen, biologischen und pharmakologischen Faktoren im Rahmen psychiatrischer Erkrankungen können die ätiologische Zuordnung sexueller Dysfunktionen erschweren. Als gesichert gilt jedoch, daß Substanzen mit vorwiegend serotonergem Wirkmechanismus mit vergleichsweise hohen Inzidenzen sexueller Nebenwirkungen assoziiert sind. Substanzen mit postsynaptischer Serotonin-2-Rezeptorblockade oder alpha-adrenolytischer Wirkkomponente zeichnen sich hingegen durch das weitgehende Fehlen sexueller Dysfunktionen aus. Innerhalb der Gruppe der Antipsychotika sind sexuelle Nebenwirkungen vor allem bei Präparaten mit ausschließlicher oder vorwiegender Dopamin-2-Rezeptorblockade und damit assoziierten endokrinologischen Nebenwirkungen zu erwarten. Bei Auftreten iatrogen induzierter Sexualstörungen sind gegenregulierende Maßnahmen stets nach strenger individueller Nutzen-Risiko-Abwägung zu treffen. Der vorliegende Artikel bietet einen Überblick über die aktuelle Literatur zu Pathophysiologie, Inzidenz und klinischem Management psychopharmakainduzierter Sexualstörungen.

  4. Instandhaltung und Normung

    Science.gov (United States)

    Rosenkranz-Wuttig, Angela

    Das DIN Deutsches Institut für Normung e.V. ist ein eingetragener Verein mit Sitz in Berlin. Das DIN erarbeitet gemeinsam mit den Experten der interessierten Kreise Normen und Standards als Dienstleistung für Wirtschaft, Staat und Gesellschaft. Die Kenntnis entsprechender Normen, Standards oder Regeln, sowie deren richtiger Einsatz, ist unter den heutigen Marktbedingungen für jedes Unternehmen ein nicht zu unterschätzender wirtschaftlicher Faktor. Normen schaffen Effizienz bei Produktionsabläufen und sorgen durch eine gleichbleibende sowie vergleichbare Produktqualität für Vertrauen bei den Endverbrauchern. Darüber hinaus stehen Normen unter rechtlichen Aspekten für mehr Sicherheit - Hersteller erreichen bei Anwendung und Einhaltung von Normen ein hohes Maß an rechtlicher Absicherung. Das DIN vertritt die deutschen Interessen in den europäischen und weltweiten Normungsorganisationen, siehe Abb. 1. Zuständig für die elektrotechnische Normung in Deutschland sowie als deutscher Repräsentant in den übernationalen Gremien ist die DKE Deutsche Kommission Elektrotechnik Elektronik Informationstechnik im DIN und VDE.

  5. Quintilian und lateinische Lautlehre

    Directory of Open Access Journals (Sweden)

    Matjaž Babič

    2010-12-01

    Full Text Available Die bekanntesten »grammatischen« Stellen aus Quintilians Institutio oratoria sind I, 4-9 und XII, 10, 27-34. Wie man hat richtig erkannt hat, schenkte er in den Kapiteln über Grammatikunterricht dem sprachlichen Teil verhältnismäßig mehr Aufmerksamkeit  als den literarischen Themen. Dass er den Grammatikunterricht ausführlicher behandelte überrascht nicht, denn Rhetorik als eine kommunikative Tätigkeit kommt ohne Sprachrichtigkeit und Sprachbewußsein nicht aus. Es gibt jedoch auch einige andere Abschnitte behandelnde Stellen, die in den Sonderabhandlungen meist beiseite gelassen werden. Vor allem seine Bemerkungen, die man hie und da im Text findet, sind eine wertvolle Quelle für die Beschreibung des lateinischen Lautsystems. Quintilian erwies als Redner und Rhetoriklehrer für den wirklichen Klang der lateinischen Sprache mehr Feingefühl als Grammmatiker, die die Sprache durch die Brille von elementa sahen und sich dem althergebrachten Normativismus verpflichtet fühlten. Dieses Sprachgefühl ermöglichte es ihm - wenn man die Stellen entsprechend interpretiert - , auch Lautveränderungen wahrzunehmen, die sich seinerzeit noch in den Ansätzen befanden und erst Jahrhunderte später - als sie die phonologische Struktur des Lateinischen beeinflussten - in den Abhandlungen der Grammatiker ihre Kodifizierung fanden.

  6. Apoptose, Inflammation und Angiogenese beim Prostatakarzinom und der benignen Prostatahyperplasie

    OpenAIRE

    Niß-Engel, Wiebke

    2010-01-01

    Sowohl die benigne Prostatahyperplasie (BPH) als auch das Prostatakarzinom (PCA) erlangen als Erkrankungen des Mannes zunehmende Bedeutung. Ziel: Untersuchungsergebnisse zu Markierungsverhalten und Verteilungsmustern verschiedener Proteine bei PCAs und BPHs. Resektate von 50 Patienten wurden immunhistochemisch mit Antikörpern gegen COX2, RANTES, TLR2, BCL2, CASP3 und -9, Fasl, MMP und VEGF gefärbt. Sowohl bei den Markern der Apoptose als auch denen der Inflammation zeigte sich ein...

  7. Mobiler Lernalltag - Erfahrungsbasiert und informell

    NARCIS (Netherlands)

    Specht, Marcus

    2013-01-01

    Specht, M. (2013, 26 April). Mobiler Lernalltag - Erfahrungsbasiert und informell. Invited talk given at the Speedlab Mobile Learning organized by the Kooperative Berlin und Bundeszentrale für politische Bildung, Hannover, Germany.

  8. Derivation of validated methods of sampling and analysis for intermediate and final products of the anaerobic material utilization of volatile chlorinated hydrocarbons (LCFC) in groundwater in the context of analyses of contaminated soils; Ableitung validierter Probenahme- und Analysenmethoden fuer Zwischen- und Endprodukte der anaeroben Stoffverwertungsprozesse von Leichtfluechtigen Chlorierten Kohlenwasserstoffen (LCKW) im Grundwasser im Rahmen von Altlastenuntersuchungen

    Energy Technology Data Exchange (ETDEWEB)

    Dorgerloh, Ute; Becker, Roland; Win, Tin [Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin (Germany); Theissen, Hubert [IMAGO GbR (Germany)

    2010-06-17

    The results of the project ''Methods of sampling and analysis of intermediate and final products of the anaerobic degradation of volatile halogenated hydrocarbons in groundwater in frame of analysis of contaminated sites'' of the German Federal States Program ''Water, Soil, Waste'' (Laenderfinanzierungsprogramm ''Wasser, Boden, Luft'') LFP B2.08 are presented in these report. Different methods of sampling and analysis for the determination of hydrogen, methane, ethene and vinyl chloride in groundwater are developed and validated: For the sampling are described and discussed: i. active sampling: purge and sample of water samples and purging of solvated gases in groundwater in gas sampling tubes ii. passive sampling: diffusion sampling in polyethylene diffusion bags (PDB) and plastic syringes as diffusion sampler for solvated gases The use of active (purge and sample, downhole sampler) and passive (diffusion sampling) sampling techniques for the quantification of VOC, ethene, and methane are evaluated from the viewpoint of public authorities and regarding the reproducibility of measurement results. Based on a groundwater contaminated with trichloroethene, 1,2-dichloroethene, and vinyl chloride it is shown that passive sampling is restricted by low groundwater flow and biological activity inside the well casing. Therefore, active sampling is to be preferred in case of unknown or insufficient flow conditions in the aquifer. The methods of chromatography for the determination of the compounds are validated and compared with other appropriate analytical methods: I. Headspace-GC-FID for the determination of methane, ethene and vinyl chloride in water of the purged sample (i) and the water of the PDB (ii) II. Direct injection - GC-PDD for the determination of hydrogen from the collected gas samples of the gas sampling tube (i) and the plastic syringes (ii) The gas chromatographic procedure for vinyl chloride using

  9. Anaerobically Grown Escherichia coli Has an Enhanced Mutation Rate and Distinct Mutational Spectra

    Science.gov (United States)

    Shewaramani, Sonal; Finn, Thomas J.; Kassen, Rees; Rainey, Paul B.

    2017-01-01

    Oxidative stress is a major cause of mutation but little is known about how growth in the absence of oxygen impacts the rate and spectrum of mutations. We employed long-term mutation accumulation experiments to directly measure the rates and spectra of spontaneous mutation events in Escherichia coli populations propagated under aerobic and anaerobic conditions. To detect mutations, whole genome sequencing was coupled with methods of analysis sufficient to identify a broad range of mutational classes, including structural variants (SVs) generated by movement of repetitive elements. The anaerobically grown populations displayed a mutation rate nearly twice that of the aerobic populations, showed distinct asymmetric mutational strand biases, and greater insertion element activity. Consistent with mutation rate and spectra observations, genes for transposition and recombination repair associated with SVs were up-regulated during anaerobic growth. Together, these results define differences in mutational spectra affecting the evolution of facultative anaerobes. PMID:28103245

  10. Wasserkraftanlagen Planung, Bau und Betrieb

    CERN Document Server

    Giesecke, Jürgen

    2005-01-01

    Dieses Standardwerk der Wasserkraft liegt hiermit bereits in 4. Auflage vor. Es wurde noch stärker an die Erfordernisse der Praxis angepasst, komplett überarbeitet, aktualisiert und - wo es angebracht war - ergänzt. Wasserkraftanlagen bedeuten Planung, Gestaltung, Ausführung und Betrieb von Bauwerken sowie hydraulischen und elektrischen Komponenten.

  11. Beruf und Alltag alleinlebender Frauen

    Directory of Open Access Journals (Sweden)

    Ellen Seßar-Karpp

    2003-11-01

    Full Text Available Ein Leben ohne Partner und Kinder könnte zu einer Erweiterung des Selbst und damit zu einer positiven Persönlichkeitsveränderung führen. Dieser Hypothese geht U. Schlicht in Interviews mit 15 alleinlebenden Akademikerinnen in Berlin nach und findet ihre Hypothese weitgehend bestätigt.

  12. Biodegradation of triclosan and formation of methyl-triclosan in activated sludge under aerobic conditions

    DEFF Research Database (Denmark)

    Chen, Xijuan; Nielsen, Jeppe Lund; Furgal, Karolina;

    2011-01-01

    Triclosan is an antimicrobial agent which is widely used in household and personal care products. Widespread use of this compound has led to the elevated concentrations of triclosan in wastewater, wastewater treatment plants (WWTPs) and receiving waters. Removal of triclosan and formation...... of triclosan- methyl was investigated in activated sludge from a standard activated sludge WWTP equipped with enhanced biological phosphorus removal. The removal was found to occur mainly under aerobic conditions while under anoxic (nitrate reducing) and anaerobic conditions rather low removal rates were...... determined. In a laboratory-scale activated sludge reactor 75% of the triclosan was removed under aerobic conditions within 150 h, while no removal was observed under anaerobic or anoxic conditions. One percent of the triclosan was converted to triclosan-methyl under aerobic conditions, less under anoxic...

  13. Sukzession und Tradition in Antike und Urchristentum

    Directory of Open Access Journals (Sweden)

    E. Bammel

    1993-01-01

    Full Text Available Succession und tradition in Antiquity and Early Christianity This article focusses on the concepts which today are known as ‘succession’ and ‘apostolic tradition’. It investigates the meaning and reference of these concepts within the context of textual evidences from Antiquity and Early Christianity. From this historical perpective, the article aims at arguing that both concepts should primarily be understood within a judicial and political realm and not merely as theological ideas.

  14. ISOLATION OF ANAEROBES IN DEEP SEATED PRESSURE ULCERS USING A NOVEL INNOVATIVE TECHNIQUE OF ANAEROBE ISOLATION

    Directory of Open Access Journals (Sweden)

    Lalbiaktluangi

    2015-12-01

    Full Text Available BACKGROUND Isolation of an anaerobe is usually neglected in hospitals with limited resources due to the expensive and complicated technique of anaerobic isolation methods, which is difficult to arrange in such resource poor settings. Conventionally adopted anaerobic culture methods such as Anaerobic jar, Gas-Pak, Anoxomat or Automated glove-box systems are extremely costly and cumbersome for single unit testing, but not suitable for small scale laboratories. However, anaerobic bacteria are not to be overlooked as they have made a comeback in clinical settings and are even showing resistance to Metronidazole, once thought to be the gold standard bullet against anaerobes. Deep seated pressure ulcers are usually the site where anaerobe causes an infection in synergy with aerobes. AIMS AND OBJECTIVES Isolation of anaerobes in deep seated pressure ulcers using a novel innovative technique and to study their antibiogram profile. MATERIALS AND METHODS Swabs taken from depth of deep seated pressure ulcers were immediately inoculated in Brucella blood agar at bedside and placed in polycarbonate airtight jar for anaerobic incubation using a novel innovative Modified Candle Jar technique. In this technique five grams of grease-free grade zero steel wool were dipped in 50ml freshly prepared acidified copper sulphate solution until the copper colour appeared. Excess solution was drained and the steel wool was moulded into a loose pad to fit on an open Petri plate placed on top of the inoculated Brucella blood agar plates. A white-wax candle was placed at the centre of this plate. A small test tube containing mixture of 0.5g sodium-bicarbonate and 0.5g magnesium carbonate was kept ready to be placed inside the jar, just after placing the inoculated plate and incubated for 48 hours. RESULTS Peptostreptococcus anaerobius and Bacteroides fragilis were successfully isolated from deep seated pressure ulcers by this method. Antibiogram studies were done using the

  15. Microbiology, ecology, and application of the nitrite-dependent anaerobic methane oxidation process

    OpenAIRE

    Li-Dong eShen; Bao-lan eHu

    2012-01-01

    Nitrite-dependent anaerobic methane oxidation (n-damo), which couples the anaerobic oxidation of methane to denitrification, is a recently discovered process observed in Candidatus Methylomirabilis oxyfera. M. oxyfera is affiliated with the NC10 phylum, a phylum having no members in pure culture. Based on the isotopic labeling experiments, it is hypothesized that M. oxyfera has an unusual intra-aerobic pathway for the production of oxygen via the dismutation of nitric oxide into dinitrogen ga...

  16. Perancangan Sistem Pengukuran pH dan Temperatur Pada Bioreaktor Anaerob Tipe Semi-Batch

    OpenAIRE

    Dimas Prasetyo Oetomo; Totok Soehartanto

    2013-01-01

    Proses pada bioreaktor dapat dilakukan secara aerob yaitu menggunakan bantuan oksigen dan anaerob yaitu tidak menggunakan bantuan oksigen. Pada penelitian ini dilakukan fermentasi  enceng gondok untuk menghasilkan biogas menggunakan bioreaktor anaerob tipe semi-batch. Enceng gondok memiliki rasio C/N sebesar 22.5 – 35.84% yang merupakan komposisi optimum untuk ekstraksi biogas. Kinerja dari bioreaktor dalam produksi biogas dipengaruhi oleh beberapa parameter seperti pH dan temperatur. Pada pe...

  17. Study of the Process of Hydraulic Mixing in Anaerobic Digester of Biogas Plant

    Directory of Open Access Journals (Sweden)

    Karaeva Julia V.

    2015-03-01

    Full Text Available Two systems of hydraulic mixing in a vertical cylindrical anaerobic digester: standard and modernised are discussed in the paper. Numerical investigations that were carried out are focused on a study of hydrodynamic processes in an aerobic digester using two various systems of hydraulic mixing as well as on analysis of the efficiency of methane fermentation process accomplished under different geometric parameters of an anaerobic digester and systems of hydraulic mixing.

  18. Calibration and validation of an activated sludge model for a pilot-scale anoxic/anaerobic/aerobic/post-anoxic process%基于缺氧/厌氧/好氧/后缺氧工艺的活性污泥模型的校正及验证

    Institute of Scientific and Technical Information of China (English)

    Xiang HU; Li XIE; Chuang MI; Dian-hai YANG

    2014-01-01

    研究目的:以中试规模的缺氧/厌氧/好氧/后缺氧污水处理工艺为研究对象,利用 BioWinTM软件平台及其内嵌的通用活性污泥数学模型,建立该工艺的模拟模型并进行校正及验证,为优化该工艺的设计及运行提供指导。创新要点:成功建立了缺氧/厌氧/好氧/后缺氧工艺的活性污泥数学模型,甄别出对模型输出有显著影响的灵敏性参数。研究方法:通过呼吸试验获取进水水质特征,利用BioWinTM软件平台及其内嵌的通用活性污泥数学模型,建立该工艺的模拟模型,利用模型缺省值进行初步稳态模拟,借助灵敏度分析方法筛选出重要的化学计量学参数及动力学参数并进行模型校正,利用校正后的模型对该工艺进行了动态模拟。重要结论:灵敏度分析可优化模型的校正过程,该工艺出水水质对异养菌(OHOs)和氨氧化菌(AOB)(YH、μmaxH、Ks、bH、μmaxA、KNH4A和baeroa,A)的灵敏度较高。校正后的模型稳态模拟出水水质(pH、COD、TN、NH4+-N、NO2--N、NO3--N和TP)控制在与实测出水相应的95%置信区间内(图5),表明稳态模型建模成功,在此基础上运行的动态模拟准确地反映了工艺实际运行中的浓度变化趋势(图6),具有良好的模拟效果。%The aim of this study was to establish an activated sludge model and to verify its predictability for use in a pilot-scale anoxic/anaerobic/aerobic/post-anoxic (modified AAO) process. Respirometric measurements were conducted to identify the influent organic characteristics and to determine the yield coefficient (YH) and decay coefficient (bH) for heterotrophic biomass (OHOs). The values obtained forYH andbH were 0.72 mg COD/mg COD and 0.275 d-1, respectively. Within the model’s cali-bration, the most influential stoichiometric and kinetic parameters were identified with the aid of sensitivity analysis. The output of the model was

  19. Bewußtseins- und Organisationsentwicklung - Rationale und nichtrationale Grundlagen, Konzepte und Realitäten

    OpenAIRE

    Epstein, Holger

    2008-01-01

    Die aktuellen Krisen der menschlichen Lebenswelten und von Mutter Erde zeigen die Dringlichkeit eines grundlegenden Wandels der Art, wie wir über Wirtschaft, Organisationen und Wissenschaft denken und wie wir mit diesem Denken unser Handeln gestalten. Die wissenschaftlichen und organisationalen Revolutionen, an deren Wiege herausragende Persönlichkeiten wie Newton, Descartes, Kopernikus, Adam Smith, Calvin, Frederick W. Taylor ... stehen, bilden nun aus ihrem Schatten heraus zunehmend existen...

  20. Altautoverwertung zwischen Staat und Markt: Bedingungen und Potentiale zur Modernisierung von Lagerhaltung und Marketing gebrauchter Autoteile

    OpenAIRE

    Lucas, Rainer

    2000-01-01

    Das Arbeitspapier diskutiert vor dem Hintergrund veränderter gesetzlicher Rahmen-und Wettbewerbsbedingungen in der Autoverwertung Ziele und Maßnahmen zurStärkung kleiner und mittelständischer Verwertungsbetriebe. Der Bereich GebrauchteAutoteile wird im Rahmen eines regionalen Fallbeispiels einer tiefergehenden Analyseunterzogen. Hieraus werden Vorschläge zur regionalen Netzwerkbildung innerhalb derBranche und neue Dienstleistungsperspektiven abgeleitet. Ein wesentliches Koope-rationsfeld ist ...

  1. Arsenic, Anaerobes, and Astrobiology

    Science.gov (United States)

    Stolz, J. F.; Oremland, R. S.; Switzer Blum, J.; Hoeft, S. E.; Baesman, S. M.; Bennett, S.; Miller, L. G.; Kulp, T. R.; Saltikov, C.

    2013-12-01

    Arsenic is an element best known for its highly poisonous nature, so it is not something one would associate with being a well-spring for life. Yet discoveries made over the past two decades have delineated that not only are some microbes resistant to arsenic, but that this element's primary redox states can be exploited to conserve energy and support prokaryotic growth ('arsenotrophy') in the absence of oxygen. Hence, arsenite [As(III)] can serve as an electron donor for chemo- or photo-autotrophy while arsenate [As(V)] will serve as an electron acceptor for chemo-heterotrophs and chemo-autotrophs. The phylogenetic diversity of these microbes is broad, encompassing many individual species from diverse taxonomic groups in the Domain Bacteria, with fewer representatives in the Domain Archaea. Speculation with regard to the evolutionary origins of the key functional genes in anaerobic arsenic transformations (arrA and arxA) and aerobic oxidation (aioB) has led to a disputation as to which gene and function is the most ancient and whether arsenic metabolism extended back into the Archaean. Regardless of its origin, robust arsenic metabolism has been documented in extreme environments that are rich in their arsenic content, such as hot springs and especially hypersaline soda lakes associated with volcanic regions. Searles Lake, CA is an extreme, salt-saturated end member where vigorous arsenic metabolism occurs, but there is no detectable sulfate-reduction or methanogenesis. The latter processes are too weak bio-energetically to survive as compared with arsenotrophy, and are also highly sensitive to the abundance of borate ions present in these locales. These observations have implications with respect to the search for microbial life elsewhere in the Solar System where volcanic-like processes have been operative. Hence, because of the likelihood of encountering dense brines in the regolith of Mars (formed by evapo-concentration) or beneath the ice layers of Europa

  2. Aerobic Microbial Respiration In Oceanic Oxygen Minimum Zones.

    Science.gov (United States)

    Kalvelage, Tim; Lavik, Gaute; Jensen, Marlene M; Revsbech, Niels Peter; Löscher, Carolin; Schunck, Harald; Desai, Dhwani K; Hauss, Helena; Kiko, Rainer; Holtappels, Moritz; LaRoche, Julie; Schmitz, Ruth A; Graco, Michelle I; Kuypers, Marcel M M

    2015-01-01

    Oxygen minimum zones are major sites of fixed nitrogen loss in the ocean. Recent studies have highlighted the importance of anaerobic ammonium oxidation, anammox, in pelagic nitrogen removal. Sources of ammonium for the anammox reaction, however, remain controversial, as heterotrophic denitrification and alternative anaerobic pathways of organic matter remineralization cannot account for the ammonium requirements of reported anammox rates. Here, we explore the significance of microaerobic respiration as a source of ammonium during organic matter degradation in the oxygen-deficient waters off Namibia and Peru. Experiments with additions of double-labelled oxygen revealed high aerobic activity in the upper OMZs, likely controlled by surface organic matter export. Consistently observed oxygen consumption in samples retrieved throughout the lower OMZs hints at efficient exploitation of vertically and laterally advected, oxygenated waters in this zone by aerobic microorganisms. In accordance, metagenomic and metatranscriptomic analyses identified genes encoding for aerobic terminal oxidases and demonstrated their expression by diverse microbial communities, even in virtually anoxic waters. Our results suggest that microaerobic respiration is a major mode of organic matter remineralization and source of ammonium (~45-100%) in the upper oxygen minimum zones, and reconcile hitherto observed mismatches between ammonium producing and consuming processes therein.

  3. Aerobic Microbial Respiration In Oceanic Oxygen Minimum Zones.

    Directory of Open Access Journals (Sweden)

    Tim Kalvelage

    Full Text Available Oxygen minimum zones are major sites of fixed nitrogen loss in the ocean. Recent studies have highlighted the importance of anaerobic ammonium oxidation, anammox, in pelagic nitrogen removal. Sources of ammonium for the anammox reaction, however, remain controversial, as heterotrophic denitrification and alternative anaerobic pathways of organic matter remineralization cannot account for the ammonium requirements of reported anammox rates. Here, we explore the significance of microaerobic respiration as a source of ammonium during organic matter degradation in the oxygen-deficient waters off Namibia and Peru. Experiments with additions of double-labelled oxygen revealed high aerobic activity in the upper OMZs, likely controlled by surface organic matter export. Consistently observed oxygen consumption in samples retrieved throughout the lower OMZs hints at efficient exploitation of vertically and laterally advected, oxygenated waters in this zone by aerobic microorganisms. In accordance, metagenomic and metatranscriptomic analyses identified genes encoding for aerobic terminal oxidases and demonstrated their expression by diverse microbial communities, even in virtually anoxic waters. Our results suggest that microaerobic respiration is a major mode of organic matter remineralization and source of ammonium (~45-100% in the upper oxygen minimum zones, and reconcile hitherto observed mismatches between ammonium producing and consuming processes therein.

  4. Enhanced selection of micro-aerobic pentachlorophenol degrading granular sludge

    Energy Technology Data Exchange (ETDEWEB)

    Lv, Yuancai, E-mail: donkey1204@hotmail.com [State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640 (China); Chen, Yuancai, E-mail: chenyc@scut.edu.cn [State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640 (China); Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, College of Environment and Energy, South China University of Technology, Guangzhou 510006 (China); Song, Wenzhe, E-mail: songwenzhe007@126.com [Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, College of Environment and Energy, South China University of Technology, Guangzhou 510006 (China); Hu, Yongyou, E-mail: ppyyhu@scut.edu.cn [State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640 (China); Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, College of Environment and Energy, South China University of Technology, Guangzhou 510006 (China)

    2014-09-15

    Graphical abstract: In this work, an aerobic column reactor was placed before the USB to maintain micro-oxygen condition in the reactor and the micro-aerobic pentachlorophenol (PCP) degrading granular sludge under oxygen-limited conditions (0.1–0.2 mg L{sup −1}) was successfully obtained. PCP degradation by the micro-aerobic system was studied and the variance of microbial community was also discussed by using PCR-DGGE analysis. - Highlights: • Micro-aerobic granular sludge was cultivated in column-type combined reactors. • PCP biodegradation, VFA accumulation and biogas production were studied. • The function of Methanogenic archaeon in the system was investigated. • Fluctuation and diversity of microbial community were discussed by DGGE analysis. • The dominated microorganisms were identified by 16S rDNA sequences. - Abstract: Column-type combined reactors were designed to cultivate micro-aerobic pentachlorophenol (PCP) degrading granular sludge under oxygen-limited conditions (0.1–0.2 mg L{sup −1}) over 39-day experimental period. Micro-aerobic granular had both anaerobic activity (SMA: 2.34 mMCH{sub 4}/h g VSS) and aerobic activity (SOUR: 2.21 mMO{sub 2}/h g VSS). Metabolite analysis results revealed that PCP was sequentially dechlorinated to TCP, DCP, and eventually to MCP. Methanogens were not directly involved in the dechlorination of PCP, but might played a vital role in stabilizing the overall structure of the granule sludge. For Eubacteria, the Shannon Index (2.09 in inoculated granular sludge) increased both in micro-aerobic granular sludge (2.61) and PCP-degradation granular sludge (2.55). However, for Archaea, it decreased from 2.53 to 1.85 and 1.84, respectively. Although the Shannon Index demonstrated slight difference between micro-aerobic granular sludge and PCP-degradation granular sludge, the Principal Component Analysis (PCA) indicated obvious variance of the microbial composition, revealing significant effect of micro-aerobic

  5. Konservative und operative Therapie bei Harninkontinenz, Deszensus und Urogenitalbschwerden

    Directory of Open Access Journals (Sweden)

    Eberhard J

    2000-01-01

    Full Text Available Urogynäkologische Beschwerden wie Harninkontinenz, Infektionen, vulvo-vaginale und vesikale Reizzustände, Juckreiz, Dyspareunie, Beckenbodenschwäche, Deszensusbeschwerden werden in irgendeiner Form bei fast jeder Frau im Laufe ihres Lebens zur behandlungsbedürftigen Krankheit. Die Ursachen dieser häufigsten Frauenleiden sind vielfältig. Eine erfolgreiche Therapie sollte auf der Polyätiologie urogynäkologischer Krankheiten aufbauen und die verschiedenen Therapiemöglichkeiten zu einem patienten- und krankheitsadaptierten Behandlungskonzept zusammenfügen. Die Bausteine der konservativen Therapie sind: Trink- und Miktionstraining, Physiotherapie mit den Hilfsmitteln Kugeln, Kegel, Elektrostimulation und Biofeedbackmethoden; Östrogene, Pessare, Infekttherapie, Intimpflege, blasenrelaxierende und andere urogynäkologische Medikamente. Der Einsatz dieser Therapiebausteine wird ausführlich besprochen. Auch wird gezeigt, wann und wie operiert werden soll, wenn die konservative Therapie nicht zum Ziel führt.

  6. Oxygen regulated gene expression in facultatively anaerobic bacteria.

    Science.gov (United States)

    Unden, G; Becker, S; Bongaerts, J; Schirawski, J; Six, S

    1994-01-01

    In facultatively anaerobic bacteria such as Escherichia coli, oxygen and other electron acceptors fundamentally influence catabolic and anabolic pathways. E. coli is able to grow aerobically by respiration and in the absence of O2 by anaerobic respiration with nitrate, nitrite, fumarate, dimethylsulfoxide and trimethylamine N-oxide as acceptors or by fermentation. The expression of the various catabolic pathways occurs according to a hierarchy with 3 or 4 levels. Aerobic respiration at the highest level is followed by nitrate respiration (level 2), anaerobic respiration with the other acceptors (level 3) and fermentation. In other bacteria, different regulatory cascades with other underlying principles can be observed. Regulation of anabolism in response to O2 availability is important, too. It is caused by different requirements of cofactors or coenzymes in aerobic and anaerobic metabolism and by the requirement for different O2-independent biosynthetic routes under anoxia. The regulation mainly occurs at the transcriptional level. In E. coli, 4 global regulatory systems are known to be essential for the aerobic/anaerobic switch and the described hierarchy. A two-component sensor/regulator system comprising ArcB (sensor) and ArcA (transcriptional regulator) is responsible for regulation of aerobic metabolism. The FNR protein is a transcriptional sensor-regulator protein which regulates anaerobic respiratory genes in response to O2 availability. The gene activator FhlA regulates fermentative formate and hydrogen metabolism with formate as the inductor. ArcA/B and FNR directly respond to O2, FhlA indirectly by decreased levels of formate in the presence of O2. Regulation of nitrate/nitrite catabolism is effected by two 2-component sensor/regulator systems NarX(Q)/NarL(P) in response to nitrate/nitrite. Co-operation of the different regulatory systems at the target promoters which are in part under dual (or manifold) transcriptional control causes the expression

  7. Karamell und Schokolade optimal

    Science.gov (United States)

    Eichhorn, Andreas

    In verschiedenen Situationen in Technik, Wirtschaft oder Politik ist man daran interessiert unter einer Anzahl von möglichen Entscheidungen die jeweils beste auszuwählen, also die optimale Entscheidung zu treffen, die den größtmöglichen Nutzen bringt. In den meisten Fällen sind Nutzen und Entscheidungsalternativen nicht exakt gegeben, Entscheidungen werden dann entweder sprachlichargumentativ ausgewählt und begründet oder gar aus dem Bauch heraus gefällt. In manchen Fällen ist es aber möglich, Entscheidungsalternativen und Nutzen in Zahlen und Formeln so auszudrücken, dass über diese Beschreibung im Prinzip die optimalen Entscheidungen und der maximale Nutzen festgelegt sind. Dabei ist zu beachten, dass bei mehreren (gleichzeitig oder hintereinander) zu treffenden Entscheidungen gewisse Abhängigkeiten zu berücksichtigen sind, bezüglich der möglichen Alternativen. Wenn beispielsweise die Umsetzung einer bestimmten (Teil-)Entscheidung mit bestimmten Kosten verbunden wäre, so stünde dieser Geldbetrag für andere Entscheidungen nicht mehr zur Verfügung.

  8. Rosetta und Yella

    Directory of Open Access Journals (Sweden)

    Urs Urban

    2009-11-01

    Full Text Available L’apparition de l’homo oeconomicus marque la fin des grands récits et par là de l’ensemble social que ceux-ci avaient légitimé. En même temps cependant il entre dans les récits littéraires – et filmiques, par exemple dans Rosetta (1999 des frères Dardenne et Yella (2007 de Christian Petzold. L’article se propose de montrer comment ces derniers réussissent à mettre en scène les apories existentielles d’un sujet produit par le dispositif économique en appliquant des procédés narratifs permettant de problématiser la narration elle-même.Mit dem Auftauchen des homo oeconomicus scheint das Ende der großen Erzählungen und des von diesen legitimierten sozialen Zusammenhangs besiegelt. Genau in diesem Moment indes beginnen sich die kleinen, die literarischen und filmischen Erzählformen für den ökonomischen Menschen zu interessieren. In dem Artikel geht es darum zu zeigen, wie die Brüder Dardenne und Christian Petzold die Aporien eines vom ökonomischen Dispositiv produzierten Subjekts in ihren Filmen Rosetta (1999 und Yella (2007 erzählerisch umsetzen und dabei genau diese erzählerische Umsetzbarkeit in Frage stellen.

  9. Patentierung und Patentlage

    Science.gov (United States)

    Herrmann, Uwe

    Gewerbliche Schutzrechte nehmen in der nationalen Rechts- und Wirtschaftsordnung sowie auch auf internationaler Ebene stetig an Bedeutung zu. Sie dienen dem Schutz geistigen Eigentums und sind für jeden Gewerbetreibenden nicht nur im Hinblick darauf von Bedeutung, eigene Rechte zu sichern, sondern auch insofern von Relevanz, dass ein Verstoß gegen Rechte Dritter zu vermeiden ist. Zu den gewerblichen Schutzrechten gehören unter anderem Kennzeichenrechte, Geschmacksmusterrechte sowie die sogenannten technischen Schutzrechte in Form des Patents sowie des Gebrauchsmusters. Die folgenden Ausführungen befassen sich ausschließlich mit den technischen Schutzrechten und geben eine kurze Einführung in die Voraussetzungen, das Entstehen und den Wegfall sowie in die Wirkungen technischer Schutzrechte. Beleuchtet wird die Situation im Wesentlichen im Hinblick auf nationale technische Schutzrechte, d. h. auf Deutsche Patente und Gebrauchsmuster sowie auf Europäische Patente, die Schutz in Deutschland entfalten. Die Möglichkeit der Erlangung von Schutzrechten im außereuropäischen Ausland wird nur am Rande gestreift.

  10. Epilepsie und polyzystisches Ovarialsyndrom

    Directory of Open Access Journals (Sweden)

    Rösing B

    2012-01-01

    Full Text Available Epilepsien sind gehäuft mit endokrinen Funktionsstörungen vergesellschaftet. Bei Frauen gehören Zyklusstörungen, Hyperandrogenismus, Gewichtszunahme und Subfertilität dazu. Die vorliegende Übersicht zeigt aktuelle Daten und pathophysiologische Vorstellungen zum Zusammenhang zwischen einer Erkrankung mit Epilepsie, ihrer Therapie, dem polyzstischen Ovarialsyndrom (PCOS, sowie assoziierten metabolischen Abweichungen. Mögliche Ursachen für das Auftreten endokriner Störungen bei Epilepsie sind (1. der direkte Einfluss temporal gelegener epileptogener Läsionen oder der antiepileptischen Medikamente (AED auf die hypothalamisch-hypophysär gonadale Achse (HHG, (2. der Einfluss von AED auf die ovarielle Funktion, (3. der Einfluss der AED auf den Steroidhormonmetabolismus inklusive ihrer Serumeiweißbindung und (4. AED-bedingte sekundäre endokrine Störungen durch Gewichtszunahme und veränderten Insulinmetabolismus. Die regelmäßige ärztliche Kontrolle klinischer endokriner Parameter wie Gewichtsentwicklung, Regeltempostörungen und Hirsutismus ist bei Frauen mit Epilepsie obligat. Einzelne auffällige Laborparameter (z. B. sinkendes sexualhormonbindendes Globulin [SHBG], ansteigende Testosteronwerte, Dysbalance der Gonadotropine luteinisierendes Hormon zu follikelstimulierendem Hormon (LH/FSH oder bildgebende Befunde (polyzystische Ovarien ohne klinisches Korrelat sollten engmaschig, zunächst 1–3-monatlich kontrolliert werden, um entstehende Endokrinopathien frühzeitig behandeln zu können.

  11. Supraleitung Grundlagen und Anwendungen

    CERN Document Server

    Buckel, Werner

    2013-01-01

    Grundlegende Konzepte und Eigenschaften von Supraleitern, die Herstellung und Entwicklung von neuen supraleitenden Materialien sowie moderne Anwendungsbereiche sind die Schwerpunktthemen des Buches. Das größte Potential zur Nutzung der Supraleitung liegt in der Energietechnik. Fast unbemerkt trägt die Einführung von Kabeln, Magneten, Transformatoren oder Lagern aus supraleitenden Materialien bei zur Effizienzsteigerung bei der Stromübertragung oder in Motoren. Durch seiner klare Sprache und zahlreiche erläuternde Abbildungen eignet sich der Band hervorragend als einführendes Lehrbuch. Der Schwierigkeitsgrad wird von Kapitel zu Kapitel gesteigert. So können auch Einsteiger ohne einschlägige Vorkenntnisse dem Stoff folgen. Dank der über 350 Literaturhinweise bekommt der Leser außerdem einen Überblick über die wichtigsten Publikationen zum Thema. Ein unverzichtbares Lehrbuch also für Physik-Studenten und Studierende der Ingenieurwissenschaften. Fachleuten in Forschung und Praxis leistet das Buch au...

  12. Metabolic characteristics of an aerobe isolated from a methylotrophic methanogenic enrichment culture

    Indian Academy of Sciences (India)

    Stephen V Rapheal; K R Swaminathan; K Lalitha

    2003-03-01

    An anaerobic methylotrophic methanogenic enrichment culture, with sustained metabolic characteristics, including that of methanation for over a decade, was the choice of the present study on interspecies interactions. Growth and methanation by the enrichment were suppressed in the presence of antibiotics, and no methanogen grown on methanol could be isolated using stringent techniques. The present study confirmed syntrophic metabolic interactions in this enrichment with the isolation of a strain of Pseudomonas sp. The organism had characteristic metabolic versatility in metabolizing a variety of substrates including alcohols, aliphatic acids, amino acids, and sugars. Anaerobic growth was favoured with nitrate in the growth medium. Cells grown anaerobically with methanol, revealed maximal nitrate reductase activity. Constitutive oxidative activity of the membrane system emerged from the high-specific oxygen uptake and nitrate reductase activities of the aerobically and anerobically grown cells respectively. Cells grown anaerobically on various alcohols effectively oxidized methanol in the presence of flavins, cofactor FAD and the methanogenic cofactor F420, suggesting a constitutive alcohol oxidizing capacity. In cells grown anaerobically on methanol, the rate of methanol oxidation with F420 was three times that of FAD. Efficient utilization of alcohols in the presence of F420 is a novel feature of the present study. The results suggest that utilization of methanol by the mixed culture would involve metabolic interactions between the Pseudomonas sp. and the methanogen(s). Methylotrophic, methanogenic partnership involving an aerobe is a novel feature hitherto unreported among anaerobic syntrophic associations and is of ecological significance.

  13. Partitioning the metabolic scope: the importance of anaerobic metabolism and implications for the oxygen- and capacity-limited thermal tolerance (OCLTT) hypothesis

    DEFF Research Database (Denmark)

    Ejbye-Ernst, Rasmus; Tirsgaard, B.; Michaelsen, Thomas Y.;

    2016-01-01

    , the methodology rarely accounts for anaerobic metabolism within the MS. Using gilthead sea bream (Sparus aurata) and Trinidadian guppy (Poecilia reticulata), this study tested for trade-offs (i) between aerobic and anaerobic components of locomotor performance; and (ii) between the corresponding components...

  14. Die biene und ihre produkte in der kunst und im alltagsleben (Fruhchristliche und byzantinische Zeit

    Directory of Open Access Journals (Sweden)

    Liveri Angeliki

    2010-01-01

    Full Text Available Die vorliegende Arbeit ist in zwei Teile geteilt: den kunst- und kulturhistorischen Teil. Im ersten Teil werden Darstellungen uber Bienen Bienenzucht, Honig und Wachs seit der fruhchristlichen bis zur spatbyzantinischen Zeit vorgefuhrt. Daher ist es als Beitrag in der "Ikonographie" der Biene wahrend dieser Zeit zu verstehen. Im kulturhistorischen Teil werden Informationen uber die Bienenzucht, den Handel mit Honig und Wachs sowie ihre vielfaltige Anwendung von der byzantinischen Gesellschaft an Hand der primaren Quellen, des Standes der Forschung und der archaologischen Funden erwahnt. Damit ist erwunscht ein moglichst gutes Bild der byzantinischen Bienenzucht wieder zu geben und die Nutzung der Bienenprodukte in Byzanz anschaulich zu machen.

  15. Reuchlin und die Kabbala

    OpenAIRE

    2008-01-01

    Auszug: Hundert Jahre nachdem Johannes Reuchlin sein wissenschaftliches Werk vollendet hatte, war der von ihm hinterlassene Eindruck auf die europäische Wissenschaft und das europäische Denken so nachdrücklich, daß er - liebe- oder vorwurfsvoll - einmal >Rabbi Capnion< genannt wurde. Und wirklich - so meint Joseph Blau in seiner Darstellung der christlichen Kabbala - habe Reuchlin der hebräischen Literatur mehr gedient als mancher ordentliche Rabbiner. Ähnlich hat Gershom Scholem, der Begründ...

  16. Anaerobic bioventing of unsaturated zone contaminated with DDT and DNT.

    Science.gov (United States)

    Shah, J K; Sayles, G D; Suidan, M T; Mihopoulos, P; Kaskassian, S

    2001-01-01

    Initial degradation of highly chlorinated compounds and nitroaromatic compounds found in munition waste streams is accelerated under anaerobic conditions followed by aerobic treatment of the degradation products. The establishment of anaerobic environment in a vadose zone can be accomplished by feeding appropriate anaerobic gas mixture, i.e., "anaerobic bioventing". The gas mixture contains an electron donor for the reduction of these compounds. Lab scale study was conducted to evaluate potential of anaerobic bioventing for the treatment of an unsaturated zone contaminated with 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and 2,4-dinitrotoluene (DNT). Hydrogen was used as the electron donor. Using the soil columns innoculate with anaerobic microorganisms, it was observed that by feeding a gas mixture of 1% hydrogen, 1% carbon dioxide and nitrogen, methanogenic conditions were established and DDT was reductively dechlorinated. 1,1-dichloro-2,2-bis(p-chlorophenyl)ethane (DDD) accumulated as the intermediate product. The half life of DDT was calculated to be 8.5 months. DNT completely disappeared after six months of operation and no intermediates could be detected.

  17. Assessment of Aerobic Exercise Adverse Effects during COPD Exacerbation Hospitalization

    Directory of Open Access Journals (Sweden)

    Caroline Knaut

    2017-01-01

    Full Text Available Introduction. Aerobic exercise performed after hospital discharge for exacerbated COPD patients is already recommended to improve respiratory and skeletal muscle strength, increase tolerance to activity, and reduce the sensation of dyspnea. Previous studies have shown that anaerobic activity can clinically benefit patients hospitalized with exacerbated COPD. However, there is little information on the feasibility and safety of aerobic physical activity performed by patients with exacerbated COPD during hospitalization. Objective. To evaluate the effects of aerobic exercise on vital signs in hospitalized patients with exacerbated COPD. Patients and Methods. Eleven COPD patients (63% female, FEV1: 34.2 ± 13.9% and age: 65 ± 11 years agreed to participate. Aerobic exercise was initiated 72 hours after admission on a treadmill; speed was obtained from the distance covered in a 6-minute walk test (6MWT. Vital signs were assessed before and after exercise. Results. During the activity systolic blood pressure increased from 125.2 ± 13.6 to 135.8 ± 15.0 mmHg (p=0.004 and respiratory rate from 20.9 ± 4.4 to 24.2 ± 4.5 rpm (p=0.008 and pulse oximetry (SpO2 decreased from 93.8 ± 2.3 to 88.5 ± 5.7% (p<0.001. Aerobic activity was considered intense, heart rate ranged from 99.2 ± 11.5 to 119.1 ± 11.1 bpm at the end of exercise (p=0.092, and patients reached on average 76% of maximum heart rate. Conclusion. Aerobic exercise conducted after 72 hours of hospitalization in patients with exacerbated COPD appears to be safe.

  18. Anaerobic biotransformation of organoarsenical pesticides monomethylarsonic acid and dimethylarsinic acid

    Science.gov (United States)

    Sierra-Alvarez, R.; Yenal, U.; Feld, J.A.; Kopplin, M.; Gandolfi, A.J.; Garbarino, J.R.

    2006-01-01

    Monomethylarsonic acid (MMAV) and dimethylarsinic acid (DMAV) are extensively utilized as pesticides, introducing large quantities of arsenic into the environment. Once released into the environment, these organoarsenicals are subject to microbial reactions. Aerobic biodegradation of MMAV and DMAV has been evaluated, but little is known about their fate in anaerobic environments. The objective of this study was to evaluate the biotransformation of MMAV and DMAV in anaerobic sludge. Biologically mediated conversion occurred under methanogenic or sulfate-reducing conditions but not in the presence of nitrate. Monomethylarsonous acid (MMAIII) was consistently observed as an important metabolite of MMAV degradation, and it was recovered in molar yields ranging from 5 to 47%. The main biotransformation product identified from DMAV metabolism was MMAV, which was recovered in molar yields ranging from 8 to 65%. The metabolites indicate that reduction and demethylation are important steps in the anaerobic bioconversion of MMAV and DMAV, respectively. ?? 2006 American Chemical Society.

  19. Nutzerorientiertes Management von materiellen und immateriellen Informationsobjekten

    OpenAIRE

    Hübsch, Chris

    2001-01-01

    Schaffung einer stabilen, erweiterbaren und skalierbaren Infrastruktur für die Bereitstellung von Diensten im Umfeld von Bibliotheken und ähnlichen wissensanbietenden Einrichtungen unter Verwendung von XML-RPC und Python.

  20. The influence of preliminary aerobic treatment on the efficacy of waste stabilisation under leachate recirculation conditions

    Directory of Open Access Journals (Sweden)

    Monika Suchowska-Kisielewicz

    2014-12-01

    Full Text Available This article presents the changes in the chemical composition of leachate and the concentrations and quantity of methane production in each individual decomposition phases, determined for untreated and after aerobic treatment of waste stabilised in anaerobic reactors with and without leachate recirculation. The research results demonstrate that leachate recirculation intensifies the decomposition of both aerobically treated and untreated waste. The methane production in the reactor with untreated, stabilised waste with recirculation was 28% higher; and in the reactor with aerobically treated waste, the methane production was 24% higher than in the reactors without recirculation. An important finding of the study is that aerobic treatment of waste prior to landfilling effectively reduces the quantity of pollutant emissions in leachate and biogas from waste and increases the availability for methane micro-organisms of organic substrates from difficult-to-decompose organic substances.

  1. Aerobic landfill bioreactor

    Science.gov (United States)

    Hudgins, Mark P; Bessette, Bernard J; March, John; McComb, Scott T.

    2000-01-01

    The present invention includes a method of decomposing municipal solid waste (MSW) within a landfill by converting the landfill to aerobic degradation in the following manner: (1) injecting air via the landfill leachate collection system (2) injecting air via vertical air injection wells installed within the waste mass; (3) applying leachate to the waste mass using a pressurized drip irrigation system; (4) allowing landfill gases to vent; and (5) adjusting air injection and recirculated leachate to achieve a 40% to 60% moisture level and a temperature between 120.degree. F. and 140.degree. F. in steady state.

  2. Editorial: Medien und soziokulturelle Unterschiede

    Directory of Open Access Journals (Sweden)

    Horst Niesyto

    2009-09-01

    Full Text Available Kinder, Jugendliche und Erwachsene nutzen Medien in sehr unterschiedlicher Weise. Insbesondere handlungstheoretische Ansätze der Medienforschung konnten überzeugend belegen, dass Menschen keine passive Zielscheibe von Medien sind, sondern diese aktiv nutzen. Medienrezeption und Medienaneignung wird als Teil sozialen Handelns verstanden, die Mediennutzung wird im Lebenskontext der Menschen verortet, es wird nach der Bedeutung der Medien im Alltag und für die Lebensbewältigung gefragt. Kulturtheoretisch motivierte Studien, die die Eigenleistungen der Individuen im Auswahlprozess und in der Konstruktion von Bedeutungen betonen, verdeutlichten eigensinnige Prozesse der Medienaneignung und eine Vielfalt medienkultureller Orientierungen. Im Bereich des Lernens mit Medien wurden die Chancen selbstgesteuerter Lernprozesse mit digitalen Medien in verschiedenen Forschungsprojekten herausgearbeitet. Ein kritischer Blick auf die Medienentwicklung und die Mediennutzung kann nicht verleugnen, dass es soziokulturelle Unterschiede und Formen sozialer Ungleichheit in der Mediennutzung gibt. In den letzten Jahren entstanden in diesem Zusammenhang vermehrt Studien zu Themenbereichen wie ‹Digital divide›, Zunahme medialer Wissens- und Bildungsklüfte, Bildungsbenachteiligung und Medienaneignung. Verschiedene Fachtagungen griffen die Thematik auf, z.B. der vom JFF Institut für Medienpädagogik in Forschung und Praxis und der Pädagogischen Hochschule Ludwigsburg/Abteilung Medienpädagogik veranstaltete Fachkongress «Soziale Ungleichheit – Medienpädagogik – Partizipation» am 17./18.10.2008 in Bonn und das von der Gesellschaft für Medienpädagogik und Kommunikationskultur e.V. (GMK veranstaltete Forum «Geteilter Bildschirm – Getrennte Welten?» am 21.–23.11.2008 in Rostock. In diesen Studien und Fachtagungen ging es darum herauszuarbeiten, worin soziokulturelle Unterschiede in der Medienaneignung bestehen, was die Gründe hierfür sind, wie diese

  3. Arbeitsgestaltung und Mitarbeiterqualifizierung

    Science.gov (United States)

    Weiss-Oberdorfer, Werner; Hörner, Barbara; Holm, Ruth; Pirner, Evelin

    Die Wertkette gliedert ein Unternehmen in strategisch relevante Tätigkeiten, um dadurch Kostenverhalten sowie vorhandene und potenzielle Differenzierungsquellen zu verstehen. Wenn ein Unternehmen diese strategisch wichtigen Aktivitäten billiger oder besser als seine Konkurrenten erledigt, verschafft es sich einen Wettbewerbsvorteil." Michael Porter, 1985

  4. Datenschutzrecht und bibliothekarische Praxis

    Directory of Open Access Journals (Sweden)

    Carlheinz Rolf Straub

    2015-03-01

    Full Text Available Bericht zur Fortbildungsveranstaltung des  vdb-Regionalverbandes Südwest zum Thema „Datenschutzrecht und bibliothekarische Praxis“  am 20. November 2014. Report of the training activity held on 20 th, November 2014 by vdb- Regionalverband Südwest.

  5. Wissensmanagement und Medienbildung

    Directory of Open Access Journals (Sweden)

    Gerhard Scheidl

    2012-09-01

    Full Text Available Die Querschnittsthematik "Medienbildung" stößt auf eine immer noch fachzentrierte Ausbildung und Schulpraxis. Mögliche Lösungen könnten Überlegungen aus dem Wissensmanagement bieten, wie Gerhard Scheidl in seinem Beitrag auf verschiedenen Ebenen erläutert.

  6. Tropenbotanik und Phylogenetik

    NARCIS (Netherlands)

    Baumann-Bodenheim, M.G.

    1958-01-01

    Es lässt sich von den Tropenfloren feststellen: 1.) Die Tropenfloren erweitern das morphologische Spektrum des Angiospermen-Systems im Bereiche der systematisch wichtigen Organisationsmerkmale durch ihren Reichtum an eigenen Familien und Gattungen ganz bedeutend. Diese Merkmale stellen oftmals relat

  7. Anaerobic oxidation of methane in grassland soils used for cattle husbandry

    Directory of Open Access Journals (Sweden)

    A. Bannert

    2012-04-01

    Full Text Available While the importance of anaerobic methane oxidation has been reported for marine ecosystems, the role of this process in soils is still questionable. Grasslands used as pastures for cattle-overwintering show an increase in anaerobic soil micro-sites caused by animal treading and excrement deposition. Therefore anaerobic potential methane oxidation activity of severely impacted soil from a cattle winter pasture was investigated in an incubation experiment under anaerobic conditions using 13C-labeled methane. We were able to detect a high microbial activity utilizing CH4 as nutrient source shown by the respiration of 13CO2. Measurements of possible terminal electron acceptors for anaerobic oxidation of methane were carried out. Soil sulfate concentrations were too low to explain the oxidation of the amount of methane added, but enough nitrate and iron(III were detected. However, only nitrate was consumed during the experiment. 13C-PLFA analyses clearly showed the utilization of CH4 as nutrient source mainly by organisms harbouring 16:1ω7 PLFAs. These lipids were found in Gram-negative microorganisms and anaerobes. The fact that these lipids are also typical for type I methanotrophs, known as aerobic methane oxidizers, might indicate a link between aerobic and anaerobic methane oxidation.

  8. Effects of 12 weeks of aerobic training on autonomic modulation, mucociliary clearance, and aerobic parameters in patients with COPD

    Science.gov (United States)

    Leite, Marceli Rocha; Ramos, Ercy Mara Cipulo; Kalva-Filho, Carlos Augusto; Freire, Ana Paula Coelho Figueira; de Alencar Silva, Bruna Spolador; Nicolino, Juliana; de Toledo-Arruda, Alessandra Choqueta; Papoti, Marcelo; Vanderlei, Luiz Carlos Marques; Ramos, Dionei

    2015-01-01

    Introduction Patients with chronic obstructive pulmonary disease (COPD) exhibit aerobic function, autonomic nervous system, and mucociliary clearance alterations. These parameters can be attenuated by aerobic training, which can be applied with continuous or interval efforts. However, the possible effects of aerobic training, using progressively both continuous and interval sessions (ie, linear periodization), require further investigation. Aim To analyze the effects of 12-week aerobic training using continuous and interval sessions on autonomic modulation, mucociliary clearance, and aerobic function in patients with COPD. Methods Sixteen patients with COPD were divided into an aerobic (continuous and interval) training group (AT) (n=10) and a control group (CG) (n=6). An incremental test (initial speed of 2.0 km·h−1, constant slope of 3%, and increments of 0.5 km·h−1 every 2 minutes) was performed. The training group underwent training for 4 weeks at 60% of the peak velocity reached in the incremental test (vVO2peak) (50 minutes of continuous effort), followed by 4 weeks of sessions at 75% of vVO2peak (30 minutes of continuous effort), and 4 weeks of interval training (5×3-minute effort at vVO2peak, separated by 1 minute of passive recovery). Intensities were adjusted through an incremental test performed at the end of each period. Results The AT presented an increase in the high frequency index (ms2) (P=0.04), peak oxygen uptake (VO2peak) (P=0.01), vVO2peak (P=0.04), and anaerobic threshold (P=0.02). No significant changes were observed in the CG (P>0.21) group. Neither of the groups presented changes in mucociliary clearance after 12 weeks (AT: P=0.94 and CG: P=0.69). Conclusion Twelve weeks of aerobic training (continuous and interval sessions) positively influenced the autonomic modulation and aerobic parameters in patients with COPD. However, mucociliary clearance was not affected by aerobic training. PMID:26648712

  9. Helicobacter pylori und Magenkarzinom

    Directory of Open Access Journals (Sweden)

    Gschwantler M

    2011-01-01

    Full Text Available Das Magenkarzinom stellt weltweit das vierthäufigste Malignom und die zweithäufigste Todesursache durch Karzinome dar. Die meisten Fälle werden immer noch in einem fortgeschrittenen Stadium mit schlechter Prognose diagnostiziert. Effektive Strategien zur Prävention und Früherkennung sind daher dringend erforderlich. Durch epidemiologische Studien konnte die kausale Beziehung zwischen einer Helicobacterpylori- (H.-p.- Infektion und einem Magenkarzinom eindeutig bewiesen werden. Dabei fördert das Bakterium die Karzinogenese über 2 Mechanismen: (1 über eine indirekte Wirkung durch Induktion einer entzündlichen Reaktion und (2 über direkte Wirkungen auf Zellen der Magenmukosa durch Modulation des Proteinstoffwechsels und Induktion von Genmutationen. Die beiden wichtigsten Virulenzfaktoren von H. p. sind die Pathogenitätsinsel Cag und das vakuolisierende Zytotoxin VacA. Unter klinischen Gesichtspunkten stellt sich die Frage, ob durch eine H.-p.-Eradikation das Risiko, ein Magenkarzinom zu entwickeln, gesenkt werden kann: Insgesamt zeigen die publizierten Studien, dass durch eine H.-p.-Eradikation die Inzidenz des Magenkarzinoms gesenkt werden kann. Durch eine Strategie, in der Normalbevölkerung auf H. p. zu screenen und im Falle einer H.-p.-Infektion eine Eradikationstherapie durchzuführen, kann jedoch nur ein verhältnismäßig geringer Anteil aller Magenkarzinome verhindert werden. Eine solche Strategie ist demnach nur in Ländern mit hoher H.-p.-Prävalenz sinnvoll. Insgesamt deuten die durch Studien in der Normalbevölkerung gewonnenen Daten darauf hin, dass das Magenkarzinomrisiko am effektivsten gesenkt werden kann, wenn die H.-p.-Eradikation frühzeitig, d. h. vor Entwicklung einer atrophen Gastritis, durchgeführt wird. Zusätzlich gibt es überzeugende Daten, dass bei Hochrisikopatienten nach endoskopischer Mukosaresektion eines Magenfrühkarzinoms das Risiko der Entwicklung eines neuerlichen Magenkarzinoms durch eine H

  10. Superkomplexe aus Algen und Cyanobakterien - Isolierung, Charakterisierung und strukturelle Untersuchung

    OpenAIRE

    Heidrich, Nicolas Georges

    2011-01-01

    Seit über 10 Jahren ist bekannt, dass in Mitochondrien von Säugetieren, Pilzen, höheren Pflanzen und Algen die Atmungskettenkomplexe I, III und IV stöchiometrisch als Proteinsuperkomplexe zusammengelagert sind. Diese Superkomplexe bestehen in den bisher bekannten Formen aus einer Kopie von Komplex I, einem Homodimer von Komplex III, welcher nur als solcher aktiv ist, und einer unterschiedlichen Anzahl (0-4) an Komplex IV. Vermutlich erfolgt der Elektronentransport zwischen den Atmungskettenko...

  11. Potential use of the organic fraction of municipal solid waste in anaerobic co-digestion with wastewater in submerged anaerobic membrane technology.

    Science.gov (United States)

    Moñino, P; Jiménez, E; Barat, R; Aguado, D; Seco, A; Ferrer, J

    2016-10-01

    Food waste was characterized for its potential use as substrate for anaerobic co-digestion in a submerged anaerobic membrane bioreactor pilot plant that treats urban wastewater (WW). 90% of the particles had sizes under 0.5mm after grinding the food waste in a commercial food waste disposer. COD, nitrogen and phosphorus concentrations were 100, 2 and 20 times higher in food waste than their average concentrations in WW, but the relative flow contribution of both streams made COD the only pollutant that increased significantly when both substrates were mixed. As sulphate concentration in food waste was in the same range as WW, co-digestion of both substrates would increase the COD/SO4-S ratio and favour methanogenic activity in anaerobic treatments. The average methane potential of the food waste was 421±15mLCH4g(-1)VS, achieving 73% anaerobic biodegradability. The anaerobic co-digestion of food waste with WW is expected to increase methane production 2.9-fold. The settleable solids tests and the particle size distribution analyses confirmed that both treatment lines of a conventional WWTP (water and sludge lines) would be clearly impacted by the incorporation of food waste into its influent. Anaerobic processes are therefore preferred over their aerobic counterparts due to their ability to valorise the high COD content to produce biogas (a renewable energy) instead of increasing the energetic costs associated with the aeration process for aerobic COD oxidation.

  12. [Role of oral cavity anaerobic microflora in the pathogenesis of mandible traumatic osteomyelitis].

    Science.gov (United States)

    Ivaniushko, T P; Tumbinskaia, L V; Smirnov, A V; Balykin, R A

    2012-01-01

    The role of conditionally pathogenic microflora in the pathogenesis of lower jaw bone traumatic osteomyelites was investigated. The different groups of aerobic and anaerobic microorganisms were detected by real-time PCR. Role of conditionally pathogenic microflora in the pathogenesis of traumatic osteomielytis was shown.

  13. Combination of aerobic and vacuum packaging to control lipid oxidation and off-odor volatiles of irradiated raw turkey breast.

    Science.gov (United States)

    Nam, K C; Ahn, D U

    2003-03-01

    Effects of the combination of aerobic and anaerobic packaging on color, lipid oxidation, and volatile production were determined to establish a modified packaging method to control quality changes in irradiated raw turkey meat. Lipid oxidation was the major problem with aerobically packaged irradiated turkey breast, while retaining characteristic irradiation off-odor volatiles such as dimethyl sulfide, dimethyl disulfide, and dimethyl trisulfide was the concern for vacuum-packaged breast during the 10-day refrigerated storage. Vacuum packaging of aerobically packaged irradiated turkey breast meat at 1 or 3 days of storage lowered the amounts of S-volatiles and lipid oxidation products compared with vacuum- and aerobically packaged meats, respectively. Irradiation increased the a-value of raw turkey breast, but exposing the irradiated meat to aerobic conditions alleviated the intensity of redness.

  14. Study on biological phosphorus removal process by Acinetobacter lwoffi: possibility to by-pass the anaerobic phase

    Energy Technology Data Exchange (ETDEWEB)

    Ghigliazza, R.; Lodi, A.; Rovatti, M. [Institute of Chemical and Process Engineering ``G.B. Bonino``, University of Genoa (Italy)

    1998-03-01

    An Acinetobacter lwoffi culture has been submitted to anaerobic/aerobic conditions in a Sequencing Batch Reactor (SBR) in order to study the ability of this strain in biological phosphorus removal process. Even by feeding a pure sodium acetate substrate, no phosphorus release has been detected during anaerobiosis, while phosphorus uptake beyond metabolic needs has been recorded during the aerobic phase; the anaerobic phase seems to have no influence on the enhanced biological phosphorus removal mechanisms. Hence aerobic batch tests have been carried out in order to verify the ability of Acinetobacter lwoffi to remove phosphorus by ``luxury uptake`` and ``overplus accumulation`` without anaerobic stress. Obtained results revealed a phosphorus removal efficiency of 75-80%. (orig.) With 5 figs., 3 tabs., 18 refs.

  15. Anaerobic Digestion and its Applications

    Science.gov (United States)

    Anaerobic digestion is a natural biological process. The initials "AD" may refer to the process of anaerobic digestion, or the built systems of anaerobic digesters. While there are many kinds of digesters, the biology is basically the same for all. Anaerobic digesters are built...

  16. The interplay between aerobic metabolism and antipredator performance: vigilance is related to recovery rate after exercise.

    Science.gov (United States)

    Killen, Shaun S; Reid, Donald; Marras, Stefano; Domenici, Paolo

    2015-01-01

    When attacked by a predator, fish respond with a sudden fast-start motion away from the threat. Although this anaerobically-powered swimming necessitates a recovery phase which is fueled aerobically, little is known about links between escape performance and aerobic traits such as aerobic scope (AS) or recovery time after exhaustive exercise. Slower recovery ability or a reduced AS could make some individuals less likely to engage in a fast-start response or display reduced performance. Conversely, increased vigilance in some individuals could permit faster responses to an attack but also increase energy demand and prolong recovery after anaerobic exercise. We examined how AS and the ability to recover from anaerobic exercise relates to differences in fast-start escape performance in juvenile golden gray mullet at different acclimation temperatures. Individuals were acclimated to either 18, 22, or 26°C, then measured for standard and maximal metabolic rates and AS using intermittent flow respirometry. Anaerobic capacity and the time taken to recover after exercise were also assessed. Each fish was also filmed during a simulated attack to determine response latency, maximum speed and acceleration, and turning rate displayed during the escape response. Across temperatures, individuals with shorter response latencies during a simulated attack are those with the longest recovery time after exhaustive anaerobic exercise. Because a short response latency implies high preparedness to escape, these results highlight the trade-off between the increased vigilance and metabolic demand, which leads to longer recovery times in fast reactors. These results improve our understanding of the intrinsic physiological traits that generate inter-individual variability in escape ability, and emphasize that a full appreciation of trade-offs associated with predator avoidance and energy balance must include energetic costs associated with vigilance and recovery from anaerobic exercise.

  17. Long term studies on the anaerobic biodegradability of MTBE and other gasoline ethers

    DEFF Research Database (Denmark)

    Waul, Christopher Kevin; Arvin, Erik; Schmidt, Jens Ejbye

    2009-01-01

    Anaerobic biodegradation of methyl tert-butyl ether (MTBE) using electron acceptors such as nitrate, Fe(III), sulfate and bicarbonate, may be more cost effective and feasible compared to aerobic treatment methods, for dealing with the MTBE problem. Currently. there are a few reports in the litera......Anaerobic biodegradation of methyl tert-butyl ether (MTBE) using electron acceptors such as nitrate, Fe(III), sulfate and bicarbonate, may be more cost effective and feasible compared to aerobic treatment methods, for dealing with the MTBE problem. Currently. there are a few reports...... to investigate the anaerobic biodegradability of MTBE and other gasoline ethers. Inoculums collected from various environments were used, along with different electron acceptors. Only one set of the batch experiments showed a 30-60% conversion of MTBE to tert-butyl alcohol under Fe(III)-reducing conditions...

  18. Genomweites Expressionsprofil skelettaler Tumore und funktionelle Analyse der Ephrine und CD52 in Osteosarkomen und Riesenzelltumoren

    OpenAIRE

    Günther, Raphaela

    2008-01-01

    Knochentumore stellen mit nur 0,2% aller menschlichen Tumore sehr seltene primäre Neoplasien des skelettalen Systems dar. Diese Dissertation beschreibt die genomweite Microarray Analyse von Osteosarkomen und Riesenzelltumoren. Mit einer Microarray Analyse von konventionellen und metastatischen Osteosarkom-Geweben verglichen zu einer Osteoblasten-Primärkultur (HOBc) wurden Gene ermittelt, die im Prozess der Entstehung und Entwicklung sowie im Verlauf der Metastasierung von Osteosarkomen...

  19. Editorial: Computerspiele und Videogames in formellen und informellen Bildungskontexten

    Directory of Open Access Journals (Sweden)

    Johannes Fromme

    2008-01-01

    Full Text Available Computer- und Videospiele sind heute ein selbstverständlicher Bestandteil der Lebenswelt vieler Kinder und Jugendlicher, aber auch von (jüngeren Erwachsenen, die mit diesen neuen Medien aufgewachsen sind. Lange Zeit haben elektronische Bildschirmspiele allenfalls sporadische Beachtung gefunden. Weder in der Medienforschung oder Medienpädagogik noch in der breiteren Öffentlichkeit waren sie ein Gegenstand von breiterem Interesse.* In den letzten knapp zehn Jahren sind Video- und Computerspiele allerdings zunehmend in den Fokus der Aufmerksamkeit gerückt. Dabei sind in der massenmedial vermittelten Öffentlichkeit vor allem Amokläufe in Schulen in einen direkten Zusammenhang mit den Vorlieben (der Täter für bestimmte Computerspiele gebracht worden. Die auch von prominenten Politikern aufgegriffene These lautete, dass gewalthaltige Spiele wie der First Person Shooter «Counterstrike» ein virtuelles Trainingsprogramm für das Töten und daher als wesentliche Ursache solcher Schulmassaker anzusehen seien. Auf der Basis dieser kausalen Wirkungsannahmen bzw. der unterstellten negativen Lern- und Trainingseffekte werden seither immer wieder Forderungen nach einem Verbot solcher «Killerspiele» oder gar nach der Verbannung aller Bildschirmmedien aus den Kinderzimmern abgeleitet. Neben solcher skandalisierter Thematisierung ist aber zunehmend auch eine nüchterne wissenschaftliche Auseinandersetzung zu konstatieren. So haben sich seit Beginn des neuen Jahrtausends die «digital game studies» als interdisziplinäres Forschungsfeld etabliert. Im Jahr 2000 wurde innerhalb der Gesellschaft für Medienwissenschaft z.B. die AG Games gebildet, die sich zu einem wichtigen deutschsprachigen Forum für die wissenschaftliche Beschäftigung mit Computerspielen entwickelt hat, und im Jahr 2002 entstand die internationale Digital Games Research Association (DiGRA, die im September 2009 ihre vierte grosse Konferenz nach 2003, 2005 und 2007 durchgeführt hat

  20. Anaerobic Digestion Foaming Causes

    OpenAIRE

    Ganidi, Nafsika

    2008-01-01

    Anaerobic digestion foaming has been encountered in several sewage treatment plants in the UK. Foaming has raised major concerns for the water utilities due to significant impacts on process efficiency and operational costs. Several foaming causes have been suggested over the past few years by researchers. However, the supporting experimental information is limited and in some cases site specific. The present report aimed to provide a better understanding of the anaerobic di...

  1. Sozialtheoretische und -interventorische Paradigmen im Licht der sozialen Netzwerk- und Unterstützungsperspektive : Konvergenzen und Herausforderungen

    OpenAIRE

    2006-01-01

    Die Netzwerkperspektive hat vielfältige theoriebezogene Bezüge zu benachbarten disziplinären und professionellen Konzepten (Sozialpädagogik/Sozialarbeit, Sozialpsychologie, Arbeits- und Betriebspsychologie, Stress- und Belastungsforschung, Rehabilitationswissenschaften, Soziale Gerontologie, Pflege- und Gesundheitswissenschaften, Gemeindepsychologie, Familienforschung, systemische Therapieansätze usw.). Innerhalb dieser disziplinären Bezuge lassen sich - implizite oder explizite - intensive B...

  2. Rheuma und Haut

    Directory of Open Access Journals (Sweden)

    Zangerl G

    2009-01-01

    Full Text Available Viele Erkrankungen aus dem rheumatischen Formenkreis manifestieren sich auch an der Haut des Patienten und können dort leicht nachgewiesen werden. Gerade bei Kollagenosen oder der Psoriasisarthritis finden sich im Rahmen der Inspektion des Hautmantels entscheidende Hinweise für die Zuordnung des Krankheitsbildes. In manchen Fällen können sich auch schwere Komplikationen ankündigen, Beispiele dafür sind eine Vaskulitis bei einer rheumatoiden Arthritis, aber auch schwere Nebenwirkungen der medikamentösen Therapien (Lyell-Syndrom und ähnliche Veränderungen. In diesem Artikel sollen daher einige der markantesten Hautläsionen bei rheumatischen Erkrankungen kurz besprochen werden.

  3. Epilepsie und Schwangerschaft

    Directory of Open Access Journals (Sweden)

    Luef G

    2009-01-01

    Full Text Available Epilepsien sind eine der häufigsten neurologischen Erkrankungen und umfassen eine Vielzahl von heterogenen Erkrankungen, welche epileptische Anfälle als gemeinsames Symptom besitzen. Schwangerschaften epilepsiekranker Frauen beschäftigen Neurologen und Gynäkologen gleich häufig, da sowohl epileptische Anfälle mit tonisch-klonischen Krämpfen, als auch deren medikamentöse Therapie teratogen sein können. Über 90 % der Schwangerschaften epilepsiekranker Frauen verlaufen weitgehend problemlos. Auch die Anfallsfrequenz ist nur bei sehr wenigen Patientinnen gesteigert. Trotzdem ist es wichtig, dass im speziellen Fall schwangerer Epilepsiepatientinnen verschiedene Fachkräfte zusammenarbeiten. Eine Epilepsie stellt also in der Regel keinen Grund dar, auf Kinder zu verzichten.

  4. Hegel und Gorgias

    DEFF Research Database (Denmark)

    Huggler, Jørgen

    Hegel und Gorgias /Jørgen Huggler (University of Aarhus, Denmark) (huggler(at)dpu.dk) Hegels Verständnis des Sophist Gorgias von Leontinoi in den Vorlesungen über die Geschichte der Philosophie gründet auf seine eigene Interpretation der Eleaten, insbesondere Zenon. Aufgrund eigener philosophischer...... Bemühungen hatte Hegel eine einzigartige Interpretation entwickelt über diese Gruppe von vorsokratischen Philosophen. Insbesonders identifizierte er eine sogenannt "objektive" immanente Dialektik bei Zenon. Nach Hegel haben die Sophisten, mit ihrem Konzept der „Bildung“ und ihren reflektierte Ansicht von...... „Gründen“, einen echten Beitrag zu dieser Entwicklung des griechischen Denkens geliefert. Vor allen scheint Gorgias die dialektischen Bemühungen der Eleaten fortgesetzt zu haben. Hegels Auslegung des Gorgias kann den lauwarmen Empfang der Sophisten in den deutschen Beiträgen zur Geschichte der Philosophie...

  5. Zystische Pankreastumoren: Diagnostik und Therapie

    Directory of Open Access Journals (Sweden)

    Siveke JT

    2013-01-01

    Full Text Available Die Gruppe der zystischen Pankreastumoren umfasst eine Vielzahl verschiedener Entitäten mit unterschiedlichen klinischen, morphologischen und prognostischen Charakteristika. Die Läsionen werden aufgrund der stetig verbesserten Bildgebung zunehmend früher und mit geringerer Größe diagnostiziert. Trotz des breiten Spektrums möglicher Ursachen entfallen 95 % aller zystischen Prozesse auf nur 5 Entitäten: Pankreaspseudozysten, intraduktale papilläre muzinöse Neoplasien (IPMN, muzinös-zystische Neoplasien (MCN, serös-zystische Neoplasien (SCN und solid-pseudopapilläre Neoplasien (SPN. Relevant ist die Unterscheidung neoplastischer zystischer Läsionen von nichtneoplastischen Zysten, wobei erstere weiter in seröse und muzinöse Neoplasien unterteilt werden, die wiederum ein unterschiedliches Malignitätspotenzial aufweisen. Die Differenzialdiagnose kann schwierig sein und beinhaltet eine genaue Anamnese, eine differenzierte bildgebende Diagnostik sowie pathologische und laborchemische Analysen. Die häufigsten neoplastischen Läsionen sind inzwischen die IPMN. Sie haben immer eine Gangkommunikation und können in Hauptgang- IPMN mit schlechterer Prognose und Seitengang- IPMN mit besserer, allerdings subtypabhängiger variabler Prognose eingeteilt werden. Die Seitengang- IPMN bedürfen einer genauen Risikoeinschätzung, die u. a. von Zystencharakteristika wie dem histologischen Subtyp und Risikofaktoren wie Größe, soliden Anteilen und Gangdilatation abhängt. Während unspezifische abdominelle Beschwerden keinen diagnostischen Wert haben, sind Gewichtsverlust, Ikterus, neu diagnostizierter Diabetes mellitus und erhöhte CA19-9- Werte im Serum Warnsymptome für eine maligne Entartung. Die Hauptgang-IPMN und geringer die MCN haben das größte Malignitätspotenzial der muzinösen Neoplasien und sollten wie die SPN immer reseziert werden, während SCN eine sehr geringes Malignitätspotenzial aufweisen. Kleinere Seitengang-IPMN und SCN k

  6. BIOESTABILIZATION ANAEROBIC SOLID WASTE ORGANIC:QUANTITATIVE ASPECTS

    Directory of Open Access Journals (Sweden)

    Valderi Duarte Leite

    2015-01-01

    Full Text Available It is estimated that in Brazil, the municipal solid waste produced are constituted on average 55% of fermentable organic solid waste and that this quantity can be applied in aerobic or anaerobic stabilization process. Anaerobic digestion is an important alternative for the treatment of different types of potentially fermentable waste, considering providing an alternative source of energy that can be used to replace fossil fuels. To perform the experimental part of this work was constructed and monitored an experimental system consisting of an anaerobic batch reactor, shredding unit of fermentable organic wastes and additional devices. Fermentable organic wastes consisted of leftover fruits and vegetables and were listed in EMPASA (Paraibana Company of Food and Agricultural Services, located in the city of Campina Grande- PB. The residues were collected and transported to the Experimental Station Biological Sewage Treatment (EXTRABES where they were processed and used for substrate preparation. The substrate consisted of a mixture of fermentable organic waste, more anaerobic sewage sludge in the proportion of 80 and 20 % respectively. In the specific case of this study, it was found that 1m3 of substrate concentration of total COD equal to 169 g L-1, considering the reactor efficiency equal to 80 %, the production of CH4 would be approximately 47.25 Nm3 CH4. Therefore, fermentable organic waste, when subjected to anaerobic treatment process produces a quantity of methane gas in addition to the partially biostabilized compound may be applied as a soil conditioning agent.

  7. Buchbesprechungen und neuere Testverfahren

    OpenAIRE

    2006-01-01

    Buchbesprechungen: Siegler, R.; DeLoache, J.; Eisenberg, N. (2005): Entwicklungspsychologie im Kindes- und Jugendalter, herausgegeben von S. Pauen. Heidelberg. Dammasch, F.; Metzger, H.-G. (Hg.) (2005): Die Bedeutung des Vaters. Psychoanalytische Perspektiven. Frankfurt a. M.. Jacobs, C.; Petermann, F. (2005): Diagnostik von Rechenstörungen. Göttingen. Katz-Bernstein, N. (2005): Selektiver Mutismus bei Kindern. Erscheinungsbilder, Diagnostik, Therapie. München. Wirschi...

  8. Einleitung: Kindheit und Gerechtigkeit

    Directory of Open Access Journals (Sweden)

    Graf Gunter

    2015-07-01

    Full Text Available Beschäftigt man sich systematisch mit der Konzeption von Gerechtigkeit für Kinder bzw. während der Kindheit, ist es hilfreich, drei verschiedene Fragen zu klären, die normalerweise in Hinblick auf Erwachsene diskutiert werden, doch auch für eine genauere Analyse der Ansprüche von Kindern relevant sind. Welche Güter sind für die Gerechtigkeitstheorie relevant? Nach welchen Prinzipien sollen diese Güter verteilt werden? Wer ist dafür verantwortlich, dass die angestrebte Güterverteilung verwirklicht wird? In dieser Einleitung umreißen wir kurz den gegenwärtigen Diskussionsstand, der in diesen drei großen gerechtigkeitsrelevanten Themenfeldern hinsichtlich von Kindern besteht. Zunächst analysieren wir Ressourcen und Fähigkeiten (capabilities als angemessene Metrik der Gerechtigkeit für Kinder. Hinsichtlich der Verteilungsprinzipien lassen sich vor allem egalitäre und suffizienztheoretische Herangehensweisen unterscheiden. Schließlich werden in Bezug auf die Frage nach den relevanten „Akteuren der Gerechtigkeit“ für Kinder („agents of justice“ hauptsächlich die Familie bzw. die Eltern und der Staat diskutiert.

  9. ...und Einstein hatte doch recht

    Science.gov (United States)

    Will, Clifford M.; Leuchs, Anne; Leuchs, Gerd

    Keine wissenschaftliche Theorie ist auf solche Faszination auch außerhalb der Wissenschaft gestoßen wie die Allgemeine Relativitätstheorie von Albert Einstein, und keine wurde so nachdrücklich mit den Mitteln der modernen Physik überprüft. Wie hat sie diesen Test mit Raumsonden, Radioastronomie, Atomuhren und Supercomputern standgehalten? Hatte Einstein recht? Mit der Autorität des Fachmanns und dem Flair des unvoreingenommenen Erzählers schildert Clifford Will die Menschen, Ideen und Maschinen hinter den Tests der allgemeinen Relativitätstheorie. Ohne Formeln und Fachjargon wird der leser mit Einsteins Gedanken vertraut und erfährt von der Bestätigung seiner Vorhersagen, angefangen bei der Lichtablenkung im Schwerefeld der Sonne 1919 bis zu den ausgefeilten Kreiselexperimenten auf dem Space Shuttle. Die Allgemeine Relativitätstheorie hat nich nur alle diese Tests bestanden, sie hat darüber hinaus wesentlich beigetragen zu unserem Verständnis von Phänomenen wie Pulsaren, Quasaren, Schwarzen Löchern und Gravitationslinsen. Dieses Buch erzählt lebendig und spannend die Geschichte einer der größten geistigen Leistungen unserer Zeit.

  10. Medium factors on anaerobic production of rhamnolipids by Pseudomonas aeruginosa SG and a simplifying medium for in situ microbial enhanced oil recovery applications.

    Science.gov (United States)

    Zhao, Feng; Zhou, Jidong; Han, Siqin; Ma, Fang; Zhang, Ying; Zhang, Jie

    2016-04-01

    Aerobic production of rhamnolipid by Pseudomonas aeruginosa was extensively studied. But effect of medium composition on anaerobic production of rhamnolipid by P. aeruginosa was unknown. A simplifying medium facilitating anaerobic production of rhamnolipid is urgently needed for in situ microbial enhanced oil recovery (MEOR). Medium factors affecting anaerobic production of rhamnolipid were investigated using P. aeruginosa SG (Genbank accession number KJ995745). Medium composition for anaerobic production of rhamnolipid by P. aeruginosa is different from that for aerobic production of rhamnolipid. Both hydrophobic substrate and organic nitrogen inhibited rhamnolipid production under anaerobic conditions. Glycerol and nitrate were the best carbon and nitrogen source. The commonly used N limitation under aerobic conditions was not conducive to rhamnolipid production under anaerobic conditions because the initial cell growth demanded enough nitrate for anaerobic respiration. But rhamnolipid was also fast accumulated under nitrogen starvation conditions. Sufficient phosphate was needed for anaerobic production of rhamnolipid. SO4(2-) and Mg(2+) are required for anaerobic production of rhamnolipid. Results will contribute to isolation bacteria strains which can anaerobically produce rhamnolipid and medium optimization for anaerobic production of rhamnolipid. Based on medium optimization by response surface methodology and ions composition of reservoir formation water, a simplifying medium containing 70.3 g/l glycerol, 5.25 g/l NaNO3, 5.49 g/l KH2PO4, 6.9 g/l K2HPO4·3H2O and 0.40 g/l MgSO4 was designed. Using the simplifying medium, 630 mg/l of rhamnolipid was produced by SG, and the anaerobic culture emulsified crude oil to EI24 = 82.5 %. The simplifying medium was promising for in situ MEOR applications.