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Sample records for aerobacter

  1. Translucent Colony Form of the Gram-Negative, Levan-Producing Bacterium, Aerobacter levanicum

    Science.gov (United States)

    Takeshita, Masaru

    1973-01-01

    A spontaneously occurring variation of Aerobacter levanicum, affecting the appearance of colonies on sucrose agar (opaque to translucent colony form), has been observed and studied. This appears to be a mutation that is accompanied by a significant increase in levansucrase (EC 2.4.1.10) activity, in levan production, and by a change in some of the properties of levansucrase. PMID:4745425

  2. Biodegradation of dichlorodiphenyltrichloroethane: intermediates in dichlorodiphenylacetic acid metabolism by aerobacter aerogenes

    Science.gov (United States)

    Wedemeyer, Gary

    1967-01-01

    The final product of dichlorodiphenyltrichloroethane (DDT) degradation by vertebrates is commonly considered to be dichlorodiphenylacetic acid, DDA. Recently, certain organisms have been found to degrade further DDA to dichlorobenzophenone (DBP), but the possibility that such degradation was due to microbial action could not be excluded. Significantly, dichlorobenzhydrol (DBH), dichlorophenylmethane (DPM), and dichlorodiphenylethylene (DDE) have been tentatively identified in rats fed DDA. Since DDA as well as DDT is degraded by the ubiquitous microorganism Aerobacter aerogenes, it seemed reasonable that the intestinal microflora might be involved in DBP formation, DPM and DBH being intermediates in its pathway from DDA. Since DDA is a (3,y-unsaturated acid, ketone formation via an alkene and an alcohol would be expected.

  3. Inhibition of Model Compound of Purple Acid Phosphatases on Growth of Aerobacter aerogenes Investigated by Microcalorimetry

    Institute of Scientific and Technical Information of China (English)

    姚俊; 刘义; 刘建本; 周琴; 秦霞; 刘鹏; 董家新; 屈松生; 喻子牛

    2003-01-01

    Microcalorimetry was used to study the inhibitory or antibiotic action of six kinds of the model compounds of purple acid phosphatases on a strain of Aerobacter aerogenes.Difference in theircapacities to inhibit the metabolism of this bacterium was observed.The extent and duration of the inhibitory effect on the metabolism as judged from the growth rate constant,k,and the half-inhibitory concentration,IC50,varied with the different drugs.The rate constank k of A.aerogenes(in the log phase) in the presence of the compounds decreased with the increasing of concentrations.The experimental results reveal that the order of the antibiotic activity of the compounds is :LD-1>LD-2>LD-3>XF-1>LD-4>LD-5.

  4. Biodegradation of ichlorodiphenyltrichloroe-thane: Intermediates in dichlorodiphenylacetic acid metabolism by Aerobacter aerogenes

    Science.gov (United States)

    1967-01-01

    The final product of dichlorodiphenyltrichloroethane (DDT) degradation by vertebrates is commonly considered to be dichlorodiphenylacetic acid, DDA (J. E. Peterson and W. H. Robison, Toxicol. Appl. Pharmacol. 6:321, 1964). Recently, certain organisms (A. S. Perry, S. Miller, and A. J. Buckner. J. Agr. Food Chem. 11:457, 1963; J. D. Pinto, M. N. Comien, and M. S. Dunn. J. Biol. Chem. 240:2148, 1965) have been found to degrade further DDA to dichlorobenzophenone (DBP), but the possibility that such degradation was due to microbial action could not be excluded. Significantly, dichlorobenzhydrol (DBH), dichlorophenylmethane (DPM), and dichlorodiphenylethylene (DDE) have been tentatively identified in rats fed DDA (Pinto et al., J. Biol. Chem. 240:2148, 1965). Since DDA as well as DDT is degraded by the ubiquitous microorganism Aerobacter aerogenes (G. Wedemeyer, Appl. Microbiol. 15:569, 1967; J. L. Mendel, and M. S. Walton, Science 151:1527, 1966), it seemed reasonable that the intestinal microflora might be involved in DBP formation, DPM and DBH being intermediates in its pathway from DDA. Since DDA is a (3,y-unsaturated acid, ketone formation via an alkene and an alcohol would be expected (S. G. Waley, Mechanisms of Organic and Enzymatic Reactions, Oxford University Press, London, England 1962).

  5. Growth of Entamoeba invadens in sediments with metabolically repressed bacteria leads to multicellularity and redefinition of the amoebic cell system.

    Science.gov (United States)

    Niculescu, Vladimir F

    2013-01-01

    Extracellular signaling and mechanisms of cell differentiation in Entamoeba are misunderstood. The main reason is the popular use of axenic media, which do not correspond to the natural habitats of Entamoeba. The axenic environment lacks the exogenous activators and repressors provided by natural habitats. Absent bacterial commensals understanding of the development of the amoebic cell system remains deficient. The present Aa(Sm) culture method using mixed sediments of antibiotically repressed Aerobacter aerogens and amoebae was developed to model in vitro extracellular signaling that induce multicellularity in cultures of E. invadens. Repressed oxygen consuming sediment bacteria supply E. invadens the hypoxic environment needed for differentiation and development. The amoebae themselves alter the environment by consuming the bacteria by phagocytosis thus reversing hypoxia. Exogenous activators are in this manner down regulated and suppressed. This feedback effect controls amoebic development and differentiation. Co-existing cell types and cell fractions with different life spans and cell cycle length could be identified. Aa(Sm) long term cultures contain continuous and non-continuous self renewing cell lines producing quiescent and terminally differentiated daughter cells (precysts) by asymmetric division. This culturing method helps to understand the intimate relationship between hypoxic environments and the multicellular behaviour of E. invadens and the interrelations existing between the distinct cell types.

  6. Application of Bacteria Cellulose in Food Industry%细菌纤维素在食品工业中的应用

    Institute of Scientific and Technical Information of China (English)

    马霞; 王瑞明; 贾士儒; 关凤梅

    2002-01-01

    @@ 细菌纤维素(Bacterical cellulose,BC)是当今国内外生物材料研究的热点之一.一般认为合成纤维素是植物特有的功能,但是,有少数微生物也能合成纤维素.现已知道,在各种不同条件下能合成纤维素的微生物有醋酸菌属(Acetobacter)、土壤杆菌属(Agrobacterium)、假单胞杆菌属(Pseudomonas)、无色杆菌属(Achrombacter)、产碱杆菌属(Alcaligenes)、气杆菌属(Aerobacter)、固氮菌属(Azotobacter)、根瘤菌属(Rhizabium)和八叠球菌属(Sarcina)等的某些种,它们合成的纤维素统称为细菌纤维素.其中真正能够批量地工业化生产细菌纤维素且合成能力最强的是醋酸菌属(Acetobacter)中的木醋杆菌(Acetobacterxylnium).

  7. The deconjugation ability of bacteria isolated from the jejunal fluids in the blind loop syndrome with high sup 14 CO sub 2 excretion. Using the breath analysis technique and thin-layer chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Shindo, K.; Yamazaki, R.; Mizuno, T.; Shionoiri, H.; Sugiyama, M. (Yokohama City Univ. School of Medicine, (Japan))

    1989-01-01

    Five patients with blind loop syndrome (Billroth II) were examined by measuring {sup 14}CO{sub 2} specific activity of expired breath samples taken at intervals after a meal containing glycine-1-{sup 14}C cholate. The 5 patients tested showed a marked increase of {sup 14}CO{sub 2} specific activity. Furthermore, the ability of deconjugation of bacteria isolated from the jejunal fluids in the efferent loop of these patients was tested by thin-layer chromatography. The bacterial species identified from the samples were as follows: enterococcus, Lactobacillus buchneri, L. bifidus, L. brevis, Eubacterium lentum, Bacteroides vulgaricus, B. filamentosum, Corynebacterium granulosum, Escherichia coli, Staphylococcus epidermidis, and Aerobacter aerogenes. These species of bacteria, except E. coli and A. aerogenes, showed the deconjugation ability by which conjugated bile acids in ox gall was hydrolyzed. Administration of chloramphenicol to the 5 patients reduced {sup 14}CO{sub 2} specific activity significantly. On the other hand, 9 healthy men who were tested showed a flat curve, and 8 of the 9 had no growth of bacteria isolated from the jejunal fluids. The remaining healthy man showed an over growth of E. coli and Pseudomonas aeruginosa, but the species did not have the ability of deconjugation.

  8. Degradation of Levan by Actinomyces viscosus

    Science.gov (United States)

    Miller, Chris H.; Somers, Penelope J. B.

    1978-01-01

    Actinomyces viscosus ATCC 15987 was examined for its ability to hydrolyze its own levan. Washed whole cells and an ammonium sulfate fraction from cell-free culture fluids were shown to possess levan hydrolase activity. Analyses of reaction mixtures by gel filtration and thin-layer chromatography demonstrated that the product of levan hydrolysis was free fructose. The cell-associated and extracellular enzyme preparations also hydrolyzed inulin and the levans synthesized by Aerobacter levanicum and Bacillus subtilis. Growth of A. viscosus in media supplemented with 0.1% A. viscosus levan resulted in a 33-fold increase and a 7-fold increase in the specific activities of the respective extracellular and cell-associated enzymes when compared with those from 55 mM glucose cultures. Growth in the presence of 29.2 mM sucrose resulted in a 28-fold increase and a 5-fold increase in the specific activities of the respective enzymes when compared with those from the glucose cultures. The extracellular enzyme exhibited high activity over a wide pH range, with 87 and 89% of its pH 6.0 optimum activity at pH 5.0 and 7.0, respectively. The cell-associated enzyme also exhibited optimum activity at pH 6.0, but this was decreased to 10 and 20% at pH 5.0 and 7.0, respectively. Analysis for the presence of extracellular levan during growth of A. viscosus in sucrose broths demonstrated that peak levan concentrations occurred during the mid-exponential to late-exponential phase of growth followed by a rapid decline in extracellular levan as a result of levan hydrolase activity. Images PMID:32137

  9. Phytochemical analysis and antimicrobial activity ofChorella vulgaris isolated from Unkal Lake

    Institute of Scientific and Technical Information of China (English)

    Shakeel Ahmed Adhoni; Basappa Basawanneppa Kaliwal

    2016-01-01

    Objective:To determine the presence of bioactive molecules and to check their antimicrobial activity from green algaeChlorella vulgaris (AS-3) (C. vulgaris) isolated from Unkal Lake in Dharwad District, Karnataka, India. Methods: Based on the polarity, benzene, chloroform, ethyl acetate, ethanol, hexane, methanol, petroleum ether and distilled water were the solvents used for the preparation of algal extracts using Soxhlet apparatus, which were further subjected to phytochemical analysis and screening of antimicrobial activity. Human pathogens such asStaphylococcus aureus, Corynebacterium,Bacillus subtilis,Streptococcus,Escherichia coli,Salmonella Paratyphi B, Klebsiella pneumoniae,Aerobacter aerogenes,Candida albicans andAspergillus niger were used for antimicrobial assay. Standard methods were followed for qualitative estimation of phytochemicals. Results: Phytochemical determination of bioactive molecules showed the presence of alkaloids, flavonoids, glycosides, carotenoids, phenols, lignins, saponins, sterols, tannins, reducing sugars, volatile oil, fats, amino acids and carbohydrates. In vitro analysis of organic solvent extracts ofC. vulgaris, a green microalgae, showed an activity by suppressing the proliferation of bacterial, fungal and human pathogens. Four extracts (chloroform, ethyl acetate, hexane and methanol) showed effective inhibitory activity against the tested pathogens. Depending on the percentage of bioactive molecules present in each of the organic extracts, different extracts showed different inhibition zone diameters against the pathogens. Among the eight organic extracts used for the study, excellent inhibitory effects were shown by chloroform and methanol extracts. Conclusions: The present study indicates that green algaeC. vulgaris is rich in natural compounds which are highly important in pharmacology and nutraceuticals. Although the presence of bioactive molecules is very less in the algae, excellent effect on the microbial pathogens was

  10. Probing interaction of Gram-positive and Gram-negative bacterial cells with ZnO nanorods

    Energy Technology Data Exchange (ETDEWEB)

    Jain, Aanchal; Bhargava, Richa; Poddar, Pankaj, E-mail: p.poddar@ncl.res.in

    2013-04-01

    In the present work, the physiological effects of the ZnO nanorods on the Gram positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli and Aerobacter aerogenes) bacterial cells have been studied. The analysis of bacterial growth curves for various concentrations of ZnO nanorods indicates that Gram positive and Gram negative bacterial cells show inhibition at concentrations of ∼ 64 and ∼ 256 μg/mL respectively. The marked difference in susceptibility towards nanorods was also validated by spread plate and disk diffusion methods. In addition, the scanning electron micrographs show a clear damage to the cells via changed morphology of the cells from rod to coccoid etc. The confocal optical microscopy images of these cells also demonstrate the reduction in live cell count in the presence of ZnO nanorods. These, results clearly indicate that the antibacterial activity of ZnO nanorods is higher towards Gram positive bacterium than Gram negative bacterium which indicates that the structure of the cell wall might play a major role in the interaction with nanostructured materials and shows high sensitivity to the particle concentration. Highlights: ► Effect of ZnO nanorods on the growth cycles of four bacterial strains. ► A relation has been established between growth rate of bacteria and concentration. ► Serious damage in the morphology of bacterial cells in the presence of ZnO nanorods. ► Microscopic studies to see the time dependent effect on bacterial cells.

  11. Phytochemical analysis and antimicrobial activity of Chorella vulgaris isolated from Unkal Lake

    Directory of Open Access Journals (Sweden)

    Shakeel Ahmed Adhoni

    2016-05-01

    Full Text Available Objective: To determine the presence of bioactive molecules and to check their antimicrobial activity from green algae Chlorella vulgaris (AS-3 (C. vulgaris isolated from Unkal Lake in Dharwad District, Karnataka, India. Methods: Based on the polarity, benzene, chloroform, ethyl acetate, ethanol, hexane, methanol, petroleum ether and distilled water were the solvents used for the preparation of algal extracts using Soxhlet apparatus, which were further subjected to phytochemical analysis and screening of antimicrobial activity. Human pathogens such as Staphylococcus aureus, Corynebacterium, Bacillus subtilis, Streptococcus, Escherichia coli, Salmonella Paratyphi B, Klebsiella pneumoniae, Aerobacter aerogenes, Candida albicans and Aspergillus niger were used for antimicrobial assay. Standard methods were followed for qualitative estimation of phytochemicals. Results: Phytochemical determination of bioactive molecules showed the presence of alkaloids, flavonoids, glycosides, carotenoids, phenols, lignins, saponins, sterols, tannins, reducing sugars, volatile oil, fats, amino acids and carbohydrates. In vitro analysis of organic solvent extracts of C. vulgaris, a green microalgae, showed an activity by suppressing the proliferation of bacterial, fungal and human pathogens. Four extracts (chloroform, ethyl acetate, hexane and methanol showed effective inhibitory activity against the tested pathogens. Depending on the percentage of bioactive molecules present in each of the organic extracts, different extracts showed different inhibition zone diameters against the pathogens. Among the eight organic extracts used for the study, excellent inhibitory effects were shown by chloroform and methanol extracts. Conclusions: The present study indicates that green algae C. vulgaris is rich in natural compounds which are highly important in pharmacology and nutraceuticals. Although the presence of bioactive molecules is very less in the algae, excellent effect

  12. ASSESSMENT OF LOCAL METHODS OF PROCESSING FOR THE PRESERVATION OF THE PHYSICO-CHEMICAL PROPERTIES AND MICROBIOLOGICAL QUALITY OF TWO LOCAL CHEESES IN ILORIN, NIGERIA

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    Bolanle Kudirat Saliu

    2014-02-01

    Full Text Available Nigerian locally produced cheese from milk, commonly known as wara is highly nutritious and highly prone to attack by spoilage and pathogenic microorganisms. Moist heat treatment and salting alone are the common methods used for processing. This study assessed the efficacy of these local processing methods in preserving the physicochemical properties and microbiological quality of wara. Samples were purchased from open markets in Ilorin; processed by boiling in water and with addition of salt; and stored at room temperature (28±2oC to mimic the local method. The samples were observed at 24 hrs interval for a period of 96 hrs, for changes in pH, color, odor and texture. Microbiological analysis was done following standard methods. The pH of most of the samples increased while the color, odor and texture deteriorated within 48 hrs of storage. The cheese samples were preserved most by boiling with salt. Many spoilage and pathogenic microorganisms were isolated. The bacterial isolates were Lactobaccillus acidophilus, Escherichia coli, Bacillus cereus, Staphylococcus aureus, Proteus vulgaris, Enterococcus feacalis, Aerobacter aerogenes, Klebsiella, Aerococcus, Micrococcus and Streptococcus spp.; while the fungi were Aspergillus flavus, A. fumigatus, A. flavus, Candida tropicalis, Rhizopus arrhizus, Penicillium and Mucor spp. Some of the microorganisms were eliminated during treatments, others survived while some contaminated the samples during storage. Though boiling of wara with salt was shown to improve its keeping quality compared to other methods studied, further treatments such as frying, drying, and roasting; as well as storage at low temperature may significantly increase the shelf life.

  13. Bacteremias por bacilos gram-negativos

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    Adrelírio J. R. Gonçalves

    1969-01-01

    Full Text Available São apresentados 31 casos de bacteremia por gram-negativos, assunto que vem merecendo muita atenção dos pesquisadores nos últimos anos. Os organismos etiológicos mais importantes que apareceram em igualdade de freqüência foram Escherichia coli e Klebsiella-Aerobacter, sendo responsáveis por 58% do total das infecções, seguidos por Pseudomonas. A porta de entrada mais freqüente foi o trato urinário em 61,3% dos casos. A infecção foi mais comum no sexo masculino e a faixa etária de 50 a 60 anos predominou. O uso prévio de antibióticos foi um fator predisponente muito importante, seguido pelo uso de esteróides e citostáticos. As principais doenças predisponentes foram diabetes mellitus e neoplasias malignas. Os principais fatores precipitantes foram a manipulação do aparelho urinário, com infecção prévia ou desencadeada, cirurgia do aparelho digestivo, uronatia obstrutiva e obstrução biliar. As principais manifestações clínicas foram a presença de febre, calafrios e hipotensão arterial. A complicação mais freqüente foi o choque bacteriano que incidiu em 58% dos casos, aproximadamente três vêzes aquela relatada na literatura. As outras foram a insuficiência renal aguda, superinfecção e infecção pulmonar metastática. Considerações terapêuticas gerais e esquemas de antibióticos são propostos para estes casos. A mortalidade da bacteremia simples foi de 30,7% e quando associada ao choque elevou-se para 72,2% . As infecções por Pseudomonas foram 100% fatais.

  14. The deconjugation ability of bacteria isolated from the jejunal fluids in the blind loop syndrome with high 14CO2 excretion--using the breath analysis technique and thin-layer chromatography.

    Science.gov (United States)

    Shindo, K; Yamazaki, R; Mizuno, T; Shionoiri, H; Sugiyama, M

    1989-01-01

    Five patients with blind loop syndrome (Billroth II) were examined by measuring 14CO2 specific activity of expired breath samples taken at intervals after a meal containing glycine-1-14C cholate. The 5 patients tested showed a marked increase of 14CO2 specific activity. Furthermore, the ability of deconjugation of bacteria isolated from the jejunal fluids in the efferent loop of these patients was tested by thin layer chromatography. The bacterial species identified from the samples were as follows: enterococcus, Lactobacillus (L) buchneri, L. bifidus, L. brevis, Eubacterium (E) lentum, Bacteroides (B) vulgaricus, B. filamentosum, Corynebacterium (C) granulosum, Escherichia (E) coli, Staphylococcus (S) epidermidis, and Aerobacter (A) aerogenes. These species of bacteria, except E. coli and A. aerogenes, showed the deconjugation ability by which conjugated bile acids in ox gall was hydrolyzed. Administration of chloramphenicol (1g per day for 14 days orally divided doses) to the 5 patients reduced 14CO2 specific activity significantly. On the other hand, 9 healthy men (control subjects) who were tested showed a flat curve, and 8 of the 9 had no growth of bacteria isolated from the jejunal fluids. The remaining healthy man showed an overgrowth of E. coli and Pseudomonas (P) aeruginosa, but the species did not have the ability of deconjugation. Thus, we concluded that the patients with blind loop syndrome(Billroth II) had the bacterial overgrowth in the efferent loop that contained species with deconjugation ability, and, as a result the bacterial overgrowth contributed to causing abnormalities (increased deconjugation) in the metabolism of bile acids in the small intestine. When the concentration of conjugated bile acids in the small intestine was reduced to levels below the critical micellar concentration by several factors, fat malabsorption and subsequent steatorrhea were induced (1,-4). Furthermore, H. Fromm and A. F. Hofmann presented in vivo that the patients

  15. 直肠癌会阴区切口骶前引流管持续冲洗及负压吸引预防感染的前瞻性研究%Prospective study on infection prevention by drain continuous washing and vacuum suction of presacral rectal cancer perineal region

    Institute of Scientific and Technical Information of China (English)

    关军民; 罗妙玲

    2016-01-01

    Objective to investigate clinical application value of infection prevention by drain continuous washing and vacuum suction of presacral rectal cancer anterior perineal region.Methods choose 87 cases rectal cancer patients with Miles surgery treated in Xinjiang Huazhong University of Science and Technology Bortala Prefecture People's Hospital Hubei Union Branch from January 2007 to November 2015, and carry on standard Miles operation through experimental and control group. Patients in experimental group were treated with presacral drainage tube and control group with pelvic and presacral drainage tube, examine materials by Chi-Square test. Results among 87 cases in study, 4 cases in experiment group occurred perineal incision infection, 11 cases in control group occurred perineal infection, difference between two groups showed statistical significance,P<0.05. Patient’s infection occurred in 3-14d after operation, and was located at presacral space. After dressing treatment, 2 cases developed chronic sinus surgery, which was resected by the second operation. Bacteria culture results of incision: 10 cases Escherichia coli, 2 cases Klebsiella pneumonia, 2 cases Aerobacter cloacae, 1 case Acinetobacter Bauman.Conclusion continuous drainage washing of presacral perineal region is simple and can effectively reduce infection rate of perineal region of rectal cancer.%目的:探讨直肠癌术后会阴区骶前引流管持续冲洗及负压吸引预防感染的临床应用价值。方法选取2007年1月到2015年11月新疆华中科技大学博州人民医院湖北协和分院收治87例行Miles手术直肠癌患者采用实验组和对照组,患者均行标准Miles手术治疗,实验组患者置骶前引流管,对照组使用盆腔、骶前引流管,进行检验,计数资料使用卡方检验,结果87例患者纳入研究,实验组术后4例患者发生会阴区切口感染,对照组11例发生会阴区感染,两组比较差异有统计学意义,P<0

  16. Surveillance of diarrheal bacteria and their epidemiological chracteristics in Yunan military area%云南战区腹泻病原菌监测及流行病学特征研究

    Institute of Scientific and Technical Information of China (English)

    李刚山; 张朝雄; 周丽华; 胡挺松; 刘德华; 王霞; 易昌清; 古良琪; 石清明; 邓波; 李丽娇; 王意银; 范泉水; 朱姝媛; 朱琼媛; 李明; 邱薇; 王惠萱; 赵丽芝; 秦海燕

    2011-01-01

    (94.25%), proteus mirabilis and Aerobacter cloacae ( 3.23% ), Klebsiella oxytoca (2.89%), Enteroinvasive E. coli, EIEC(2.21%) and Aeromonas(1.53% ). The detection rate in different age groups were in the range of 29.11%~48.34%,being higher in the age groop of from 16 yens to 45 years and highest in the age groups of 36 years to 445 years. There was no differences between sexes in positive rate(P>0.05). May, August and September were the months with higher positive rate.Conclusion The list of diarrheal pathogens and the characteristic of the pathogens to have provided important rinformation for control of the pathogens

  17. 533例汶川地震伤员感染流行病学分析与思考%Epidemiological analysis and thinking on infections in the 533 trauma patients following Chinese Wenchuan earthquake

    Institute of Scientific and Technical Information of China (English)

    杨策; 周林; 黄旭东; 朱佩芳; 王正国; 蒋建新; 钟河江; 蒋电明; 张连阳; 王爱民; 蒋东坡; 都定元; 胡平; 刘丁

    2008-01-01

    and clinical infection investigation were documented. Results Of 533 patients, the number of the patients whose ISS is below 16 is 456 (83.6%), the number between 16 and 25 is 65 (12.2%), and the humor above 25 is 12 (2.3%). The patients were classfled based on their fracture parts as follows: head and neck (n = 42), face (n = 7), chest (n = 114), abdominal and cavitas pelvis (n =81), limb and pelvis (n =314), body surface (n =205), with 180 single fracture site, 139 of them being two combined fracture sites, and 114 of them being above three combined fracture sites. Thirty-two of the patients were suffered from amputation. The number of patients suffered from crushing syndrome reached 21, with 281 surgical operations in hospitals. Seventy-nine patients were suffered from infections including 87.3% of pre-hespital infections. The results from bacteria culture and antibiotic susceptibility showed that the infected bacteria mainly involved in Escherichia coli, Staphylococcus anreus, Staphylococcus haemolyticns, Klebsiella pneumoniae, Baumanii, Aerobacter cloacae, Pseudomonas aeruginosa, C type chain coccus, Bacillus aerogenes capsulatus. The antibiotic susceptibility to diverse bacteria has no obvious changes and exists partial overlapping, and infected patients should be given the treatment of cephalosporin, macrolide antibiotic and so on. Conclusions For the emergency conditions after the catastrophe, the comprehensive hospitals must be prepared to meet large quantities of severe trauma and infection therapy. The scientific selection of antibiotics in the combinative therapy is of great importance to the enhancement of early specific treatment, prevention of severe trauma complications and rehabilitation of patients.