WorldWideScience

Sample records for aeg pruefreaktor pr-10

  1. AEG System

    Data.gov (United States)

    Department of Transportation — The AEG System is used to create, revise, approve, and distribute text of the AEGS and Flight Standard Board (FSB)/Type Rating Report. The MMEL specifies under what...

  2. Lõigutud aeg / Vappu Thurlow

    Index Scriptorium Estoniae

    Thurlow, Vappu, 1960-

    2007-01-01

    Adamson-Ericu muuseumis kuni 25. XI avatud näitusest "Lõigutud aeg". Henri Bergsoni ajakäsitlusest. Töödest: Anu Juuraku "Suplejad", Ilkka Väätti "Kinaalda" ja "Ton", Aili Vahtrapuu "Vaagen vabale vaimule", Marje Üksise "Nii ma lähen", Annemie Bogaerts'i "Lõpp on vaikus", Siim-Tanel Annuse "24 tundi", Lynne Allen'i "Saak", Eve Kiileri "Ilu etalonide arhiivid", Ingrid Ledent'i "Mälu kestev olemine, mis muudab mineviku olevikuks", Raoul Kurvitza "Aeg ja lugu"

  3. AEgIS installation completed

    CERN Multimedia

    Katarina Anthony

    2012-01-01

    Gravity. Despite first being described over three centuries ago, it remains one of the least understood of the fundamental forces explored by physicists. At CERN’s recently completed AEgIS experiment, a team has set out to examine the effect of gravity on an as-yet-uncharted realm: antimatter.   The complete AEgIS set-up. Located in the AD hall, the AEgIS experiment plans to  make the first direct measurement of Earth’s gravitation effect on antimatter. By sending a beam of antihydrogen atoms through very thin gratings, the experiment will be able to measure how far the antihydrogen atoms fall and in how much time – giving the AEgIS team a measurement of the gravitational coupling. “By the end of 2012, we had finished by putting all the elements of the experiment together,” explains Michael Doser, AEgIS Spokesperson. “Now we have to show that they can all work together and, unfortunately, we will have no antiproton beams fo...

  4. The AEgIS experiment

    Energy Technology Data Exchange (ETDEWEB)

    Testera, G., E-mail: testera@ge.infn.it [Istituto Nazionale Fisica Nucleare (Italy); Aghion, S. [Politecnico of Milano (Italy); Amsler, C.; Ariga, A.; Ariga, T. [University of Bern, Laboratory for High Energy Physics, Albert Einstein Center for Fundamental Physics (Switzerland); Belov, A. [Institute for Nuclear Research of the Russian Academy of Science (Russian Federation); Bonomi, G. [University of Brescia, Department of Mechanical and Industrial Engineering (Italy); Braunig, P. [Heidelberg University, Kirchhoff-Institute for Physics (Germany); Bremer, J. [CERN, Physics Department (Switzerland); Brusa, R. [University of Trento, Department of Physics (Italy); Cabaret, L. [University of Paris-Sud, ENS Cachan, Laboratory Aimé Cotton, CNRS (France); Caccia, M. [INFN Milano (Italy); Caravita, R. [University of Genova, Department of Physics (Italy); Castelli, F. [INFN Milano (Italy); Cerchiari, G. [Max Planck Institute for Nuclear Physics (Germany); Chlouba, K. [Czech Technical University, Prague (Czech Republic); Cialdi, S. [INFN Milano (Italy); Comparat, D. [University of Paris-Sud, ENS Cachan, Laboratory Aimé Cotton, CNRS (France); Consolati, G. [Politecnico of Milano (Italy); Curreli, S. [Istituto Nazionale Fisica Nucleare (Italy); Collaboration: (AEgIS Collaboration); and others

    2015-08-15

    The AEgIS experiment is presently almost completely installed at CERN. It is currently taking data with antiprotons, electrons and positrons. The apparatus is designed to form a cold, pulsed beam of antihydrogen to measure the Earth’s gravitational acceleration g on antimatter and to perform spectroscopy measurements. This paper describes the main features of the apparatus and shows a selected review of some achieved results.

  5. AEgIS experiment commissioning at CERN

    Energy Technology Data Exchange (ETDEWEB)

    Krasnicky, D. [University of Genoa, Dept of Physics, Via Dodecaneso 33, 16146 Genova, Italy and Istituto Nazionale di Fisica Nucleare, Sez. di Genova, Via Dodecaneso 33, 16146 Genova (Italy); Aghion, S. [Politecnico di Milano, LNESS and Dept of Physics, Via Anzani 42, 22100 Como, Italy and Istituto Nazionale di Fisica Nucleare, Sez. di Milano, Via Celoria 16, 20133 Milano (Russian Federation); Amsler, C.; Ariga, A.; Ariga, T.; Ereditato, A.; Kawada, J.; Kimura, M.; Pistillo, C.; Scampoli, P.; Storey, J. [Albert Einstein Center for Fundamental Physics, University of Bern, 3012 Bern (Switzerland); Belov, A. S.; Gninenko, S. N. [Institute for Nuclear Research of the Russian Academy of Sciences, Moscow 117312 (Russian Federation); Bonomi, G.; Subieta Vasquez, M. A. [University of Brescia, Dept of Mech. and Indust. Engineering, Via Branze 38, 25133 Brescia, Italy and Istituto Nazionale di Fisica Nucleare, Sez. di Pavia, Via Agostino Bassi 6, 27100 Pavia (Italy); Braeunig, P.; Oberthaler, M. K. [University of Heidelberg, Kirchhoff Institute for Physics, Im Neuenheimer Feld 227, 69120 Heidelberg (Germany); Brusa, R. S.; Di Noto, L.; Mariazzi, S. [Dipartimento di Fisica, Universita di Trento and INFN, Gruppo collegato di Trento, Via Sommarive 14, 38050 Povo, Trento (Italy); and others

    2013-03-19

    The AEgIS Experiment is an international collaboration based at CERN whose aim is to perform the first direct measurement of the gravitational acceleration g of antihydrogen in the gravitational field of the Earth. Cold antihydrogen will be produced with a pulsed charge exchange reaction in a cylindrical Penning trap where antiprotons will be cooled to 100mK. The cold antihydrogen will be produced in an excited Rydberg state and subsequently formed into a beam. The deflection of the antihydrogen beam will be measured by using Moire deflectometer gratings. After being approved in late 2008, AEgIS started taking data in a commissioning phase early 2012. This report presents an overview of the AEgIS experiment, describes its current status and shows the first measurements on antiproton catching and cooling in the 5 T Penning catching trap. We will also present details on the techniques needed for the 100mK antihydrogen production, such as pulsed positronium production and its excitation with lasers.

  6. Liikluses huligaanitsemine toob karmi karistuse / Raivo Aeg

    Index Scriptorium Estoniae

    Aeg, Raivo, 1962-

    2007-01-01

    Ilmunud ka: Põhjarannik, 22. mai 2007, lk. 2; Severnoje Poberezhje, 22. mai 2007, lk. 2; Oma Saar, 23. mai 2007, lk. 5; Postimees, 23. mai 2007, lk. 16; SL Õhtuleht, 23. mai 2007, lk. 12; Virumaa Teataja, 24. mai 2007, lk. 11; Vooremaa, 24. mai 2007, lk. 2; Koit, 24. mai 2007, lk. 6; Lõunaleht, 24. mai 2007, lk. 2; Võrumaa Teataja, 24. mai 2007, lk. 2; Den za Dnjom, 25. mai 2007, lk. 19; Harjumaa, 25. mai 2007, lk. 2; Vali Uudised, 25. mai 2007, lk. 2; Lääne Elu, 26. mai 2007, lk. 4; Järva Teataja, 29. mai 2007, lk. 2; Narvskaja Nedelja, 2. juuni 2007, lk. 4. Politseiameti peadirektor Raivo Aeg liiklusjärelevalve tõhustamisest, liikluskeskkonna ohutumaks muutmisest

  7. Erakordne aeg või valed mudelid? / Erik Aru

    Index Scriptorium Estoniae

    Aru, Erik

    2008-01-01

    Paul De Grauwe, Leonardo Iania ja Pablo Rovira Kaltwasser arvutasid välja börsidel erakordsemate päevade esinemise tõenäosuse. Nende järelduse kohaselt ei ole mitte praegune aeg erakordne, vaid finantsmudelid on valed. Lisa: Indeks Dow Jones Industrial Average oktoobrikuus

  8. Genome sequencing and annotation of multidrug resistant Mycobacterium tuberculosis (MDR-TB PR10 strain

    Directory of Open Access Journals (Sweden)

    Mohd Zakihalani A. Halim

    2016-03-01

    Full Text Available Here, we report the draft genome sequence and annotation of a multidrug resistant Mycobacterium tuberculosis strain PR10 (MDR-TB PR10 isolated from a patient diagnosed with tuberculosis. The size of the draft genome MDR-TB PR10 is 4.34 Mbp with 65.6% of G + C content and consists of 4637 predicted genes. The determinants were categorized by RAST into 400 subsystems with 4286 coding sequences and 50 RNAs. The whole genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number CP010968.

  9. Antihydrogen physics: gravitation and spectroscopy in AEgIS

    CERN Document Server

    Ferragut, R; Stroke, H H; Dassa, L; Rohne, O; Hogan, S D; Cialdi, S; Al-Qaradawi, I Y; Formaro, L; Sandaker, H; Testera, G; Stepanov, S V; Folegati, P; Morhard, C; Warring, U; Prelz, F; Belov, A S; Bonomi, G; Consolati, G; Ferrari, G; Lagomarsino, V; Krasnicky, D; Drobychev, G; Giammarchi, M G; Heyne, R; Zenoni, A; Castelli, F; Mariazzi, S; Cabaret, L; Fischer, A; Boscolo, I; Sillou, D; Villa, F; Perini, D; Djourelov, N; Zavatarelli, S; Oberthaler, M K; Manuzio, G; Carraro, C; Matveev, V A; Jorgensen, L V; Nedelec, P; Prevedelli, M; Comparat, D; Dupasquier, A; Turbabin, A V; Zvezhinskij, D S; Fontana, A; Kellerbauer, A; Canali, C; Dudarev, A; Petracek, V; Riccardi, C; Nebbia, G; Gninenko, S N; Brusa, R S; Rotondi, A; Sacerdoti, M; Calloni, A; Doser, M; Byakov, V M; Quasso, F; Trezzi, D

    2011-01-01

    AEgIS (Antimatter experiment: gravity, interferometry, spectroscopy) is an experiment approved by CERN with the goal of studying antihydrogen physics. In AEgIS, antihydrogen will be produced by charge exchange reactions of cold antiprotons with positronium atoms excited in a Rydberg state (n > 20). In the first phase of the experiment, controlled acceleration by an electric field gradient (Stark effect) and subsequent measurement of free fall in a Moire deflectometer will allow a test of the weak equivalence principle. In a second phase, the antihydrogen will be slowed, confined, and laser-cooled to perform CPT studies and detailed spectroscopy. In the present work, after a general description of the experiment, the present status of advancement will be reviewed, with special attention to the production and excitation of positronium atoms.

  10. Evaluation of the Allergenicity Potential of TcPR-10 Protein from Theobroma cacao

    Science.gov (United States)

    Cardoso, Thyago Hermylly Santana; Pirovani, Carlos Priminho; Micheli, Fabienne; Noronha, Fátima Soares Motta; Alves, Andréa Catão; Faria, Ana Maria Caetano; da Silva Gesteira, Abelmon

    2012-01-01

    Background The pathogenesis related protein PR10 (TcPR-10), obtained from the Theobroma cacao-Moniliophthora perniciosa interaction library, presents antifungal activity against M. perniciosa and acts in vitro as a ribonuclease. However, despite its biotechnological potential, the TcPR-10 has the P-loop motif similar to those of some allergenic proteins such as Bet v 1 (Betula verrucosa) and Pru av 1 (Prunus avium). The insertion of mutations in this motif can produce proteins with reduced allergenic power. The objective of the present work was to evaluate the allergenic potential of the wild type and mutant recombinant TcPR-10 using bioinformatics tools and immunological assays. Methodology/Principal Findings Mutant substitutions (T10P, I30V, H45S) were inserted in the TcPR-10 gene by site-directed mutagenesis, cloned into pET28a and expressed in Escherichia coli BL21(DE3) cells. Changes in molecular surface caused by the mutant substitutions was evaluated by comparative protein modeling using the three-dimensional structure of the major cherry allergen, Pru av 1 as a template. The immunological assays were carried out in 8–12 week old female BALB/c mice. The mice were sensitized with the proteins (wild type and mutants) via subcutaneous and challenged intranasal for induction of allergic airway inflammation. Conclusions/Significance We showed that the wild TcPR-10 protein has allergenic potential, whereas the insertion of mutations produced proteins with reduced capacity of IgE production and cellular infiltration in the lungs. On the other hand, in vitro assays show that the TcPR-10 mutants still present antifungal and ribonuclease activity against M. perniciosa RNA. In conclusion, the mutant proteins present less allergenic potential than the wild TcPR-10, without the loss of interesting biotechnological properties. PMID:22768037

  11. Evaluation of the allergenicity potential of TcPR-10 protein from Theobroma cacao.

    Directory of Open Access Journals (Sweden)

    Sara Pereira Menezes

    Full Text Available BACKGROUND: The pathogenesis related protein PR10 (TcPR-10, obtained from the Theobroma cacao-Moniliophthora perniciosa interaction library, presents antifungal activity against M. perniciosa and acts in vitro as a ribonuclease. However, despite its biotechnological potential, the TcPR-10 has the P-loop motif similar to those of some allergenic proteins such as Bet v 1 (Betula verrucosa and Pru av 1 (Prunus avium. The insertion of mutations in this motif can produce proteins with reduced allergenic power. The objective of the present work was to evaluate the allergenic potential of the wild type and mutant recombinant TcPR-10 using bioinformatics tools and immunological assays. METHODOLOGY/PRINCIPAL FINDINGS: Mutant substitutions (T10P, I30V, H45S were inserted in the TcPR-10 gene by site-directed mutagenesis, cloned into pET28a and expressed in Escherichia coli BL21(DE3 cells. Changes in molecular surface caused by the mutant substitutions was evaluated by comparative protein modeling using the three-dimensional structure of the major cherry allergen, Pru av 1 as a template. The immunological assays were carried out in 8-12 week old female BALB/c mice. The mice were sensitized with the proteins (wild type and mutants via subcutaneous and challenged intranasal for induction of allergic airway inflammation. CONCLUSIONS/SIGNIFICANCE: We showed that the wild TcPR-10 protein has allergenic potential, whereas the insertion of mutations produced proteins with reduced capacity of IgE production and cellular infiltration in the lungs. On the other hand, in vitro assays show that the TcPR-10 mutants still present antifungal and ribonuclease activity against M. perniciosa RNA. In conclusion, the mutant proteins present less allergenic potential than the wild TcPR-10, without the loss of interesting biotechnological properties.

  12. Emulsion detectors for the antihydrogen detection in AEgIS

    International Nuclear Information System (INIS)

    The AEgIS experiment at CERN aims to perform the first direct measurement of gravitational interaction between matter and antimatter by measuring the deviation of a cold antihydrogen beam in the Earth gravitational field. The design of the experiment has been recently updated to include emulsion films as position sensitive detector. The submicrometric position accuracy of emulsions leads indeed to a significant improvement of the experimental sensitivity. We present results of preliminary tests and discuss perspectives for the final measurement

  13. Emulsion detectors for the antihydrogen detection in AEgIS

    Energy Technology Data Exchange (ETDEWEB)

    Pistillo, C., E-mail: ciro.pistillo@cern.ch [University of Bern, Albert Einstein Center for Fundamental Physics, Laboratory for High Energy Physics (Switzerland); Aghion, S. [Politecnico of Milano (Italy); Amsler, C.; Ariga, A.; Ariga, T. [University of Bern, Albert Einstein Center for Fundamental Physics, Laboratory for High Energy Physics (Switzerland); Belov, A. [Institute for Nuclear Research of the Russian Academy of Science (Russian Federation); Bonomi, G. [University of Brescia, Department of Mechanical and Industrial Engineering (Italy); Bräunig, P. [Heidelberg University, Kirchhoff-Institute for Physics (Germany); Bremer, J. [CERN, Physics Department (Switzerland); Brusa, R. S. [University of Trento, Department of Physics (Italy); Cabaret, L. [University of Paris-Sud, Laboratory Aim Cotton, CNRS (France); Caccia, M. [INFN Milano (Italy); Caravita, R. [University of Genova, Department of Physics (Italy); Castelli, F. [INFN Milano (Italy); Cerchiari, G. [Max Planck Institute for Nuclear Physics (Germany); Chlouba, K. [Czech Technical University (Czech Republic); Cialdi, S. [INFN Milano (Italy); Comparat, D. [University of Paris-Sud, Laboratory Aim Cotton, CNRS (France); Consolati, G. [Politecnico of Milano (Italy); Demetrio, A. [Heidelberg University, Kirchhoff-Institute for Physics (Germany); and others

    2015-08-15

    The AEgIS experiment at CERN aims to perform the first direct measurement of gravitational interaction between matter and antimatter by measuring the deviation of a cold antihydrogen beam in the Earth gravitational field. The design of the experiment has been recently updated to include emulsion films as position sensitive detector. The submicrometric position accuracy of emulsions leads indeed to a significant improvement of the experimental sensitivity. We present results of preliminary tests and discuss perspectives for the final measurement.

  14. Lõigutud aeg - "Impact 5" / Vappu Thurlow, Loit Jõekalda

    Index Scriptorium Estoniae

    Thurlow, Vappu, 1960-

    2006-01-01

    17.-21. 10. 2007 toimub Kumu Kunstimuuseumis rahvusvaheline graafikakonverents "Impact 5". Teema "Slices of Time / Lõigutud aeg" on tuletatud Henri Bergsoni filosoofiast. 4. XII toimus Adamson-Ericu muuseumis "Impact'i"-teemaline seminar. "Impact 5" logokonkursi võitis Heino Prunsvelt, II - Aapo Pukk. Kuraatorid tutvustavad küsimusi, mis võiksid olla mõtlemisaineks seoses "Impact'i" ja praeguse olukorraga ning tutvustavad lühidalt Henri Bergsoni filosoofilisi vaateid

  15. Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC).

    Science.gov (United States)

    Srivastava, Jyoti; Robertson, Chadia L; Gredler, Rachel; Siddiq, Ayesha; Rajasekaran, Devaraja; Akiel, Maaged A; Emdad, Luni; Mas, Valeria; Mukhopadhyay, Nitai D; Fisher, Paul B; Sarkar, Devanand

    2015-06-19

    Non-thyroidal illness syndrome (NTIS), characterized by low serum 3,5,3'-triiodothyronine (T3) with normal l-thyroxine (T4) levels, is associated with malignancy. Decreased activity of type I 5'-deiodinase (DIO1), which converts T4 to T3, contributes to NTIS. T3 binds to thyroid hormone receptor, which heterodimerizes with retinoid X receptor (RXR) and regulates transcription of target genes, such as DIO1. NF-κB activation by inflammatory cytokines inhibits DIO1 expression. The oncogene astrocyte elevated gene-1 (AEG-1) inhibits RXR-dependent transcription and activates NF-κB. Here, we interrogated the role of AEG-1 in NTIS in the context of hepatocellular carcinoma (HCC). T3-mediated gene regulation was analyzed in human HCC cells, with overexpression or knockdown of AEG-1, and primary hepatocytes from AEG-1 transgenic (Alb/AEG-1) and AEG-1 knock-out (AEG-1KO) mice. Serum T3 and T4 levels were checked in Alb/AEG-1 mice and human HCC patients. AEG-1 and DIO1 levels in human HCC samples were analyzed by immunohistochemistry. AEG-1 inhibited T3-mediated gene regulation in human HCC cells and mouse hepatocytes. AEG-1 overexpression repressed and AEG-1 knockdown induced DIO1 expression. An inverse correlation was observed between AEG-1 and DIO1 levels in human HCC patients. Low T3 with normal T4 was observed in the sera of HCC patients and Alb/AEG-1 mice. Inhibition of co-activator recruitment to RXR and activation of NF-κB were identified to play a role in AEG-1-mediated down-regulation of DIO1. AEG-1 thus might play a role in NTIS associated with HCC and other cancers.

  16. Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC)*

    Science.gov (United States)

    Srivastava, Jyoti; Robertson, Chadia L.; Gredler, Rachel; Siddiq, Ayesha; Rajasekaran, Devaraja; Akiel, Maaged A.; Emdad, Luni; Mas, Valeria; Mukhopadhyay, Nitai D.; Fisher, Paul B.; Sarkar, Devanand

    2015-01-01

    Non-thyroidal illness syndrome (NTIS), characterized by low serum 3,5,3′-triiodothyronine (T3) with normal l-thyroxine (T4) levels, is associated with malignancy. Decreased activity of type I 5′-deiodinase (DIO1), which converts T4 to T3, contributes to NTIS. T3 binds to thyroid hormone receptor, which heterodimerizes with retinoid X receptor (RXR) and regulates transcription of target genes, such as DIO1. NF-κB activation by inflammatory cytokines inhibits DIO1 expression. The oncogene astrocyte elevated gene-1 (AEG-1) inhibits RXR-dependent transcription and activates NF-κB. Here, we interrogated the role of AEG-1 in NTIS in the context of hepatocellular carcinoma (HCC). T3-mediated gene regulation was analyzed in human HCC cells, with overexpression or knockdown of AEG-1, and primary hepatocytes from AEG-1 transgenic (Alb/AEG-1) and AEG-1 knock-out (AEG-1KO) mice. Serum T3 and T4 levels were checked in Alb/AEG-1 mice and human HCC patients. AEG-1 and DIO1 levels in human HCC samples were analyzed by immunohistochemistry. AEG-1 inhibited T3-mediated gene regulation in human HCC cells and mouse hepatocytes. AEG-1 overexpression repressed and AEG-1 knockdown induced DIO1 expression. An inverse correlation was observed between AEG-1 and DIO1 levels in human HCC patients. Low T3 with normal T4 was observed in the sera of HCC patients and Alb/AEG-1 mice. Inhibition of co-activator recruitment to RXR and activation of NF-κB were identified to play a role in AEG-1-mediated down-regulation of DIO1. AEG-1 thus might play a role in NTIS associated with HCC and other cancers. PMID:25944909

  17. Preparing for a Professional Career in the Geosciences with AEG

    Science.gov (United States)

    Barry, T.; Troost, K. G.

    2012-12-01

    The Association of Environmental and Engineering Geologists offers multiple resources to students and faculty about careers in the geosciences, such as description of what employers are looking for, career options, mentoring, and building your professional network. Our website provides easy access to these and other resources. Most of AEG's 3000 members found their first job through association with another AEG member and more than 75% of our membership is working in applied geoscience jobs. We know that employers are looking for the following qualities: passion for your career and the geosciences, an enthusiastic personality, flexibility, responsibility, ability to communicate well in oral and written modes, and the ability to work well in teams or independently. Employers want candidates with a strong well-rounded geoscience education and the following skills/experience: attendance at field camp, working knowledge of field methodologies, strong oral and written communication skills, basic to advanced computer skills, and the ability to conduct research. In addition, skill with GIS applications, computer modeling, and 40-hour OSHA training are desired. The most successful technique for finding a job is to have and use a network. Students can start building their network by attending regular AEG or other professional society monthly meetings, volunteering with the society, attending annual meetings, going on fieldtrips and participating in other events. Students should research what kind of job they want and build a list of potential preferred employers, then market themselves to people within those companies using networking opportunities. Word-of-mouth sharing of job openings is the most powerful tool for getting hired, and if students have name recognition established within their group of preferred employers, job interviews will occur at a faster rate than otherwise.

  18. Molecular cloning and expression analysis of hyp-1 type PR-10 family genes in Hypericum perforatum

    Directory of Open Access Journals (Sweden)

    Katja eKarppinen

    2016-04-01

    Full Text Available Hypericum perforatum L. is an important medicinal plant for the treatment of depression. The plant contains bioactive hypericins that accumulate in dark glands present especially in reproductive parts of the plant. In this study, pathogenesis-related class 10 (PR-10 family genes were identified in H. perforatum, including three previously unidentified members with sequence homology to hyp-1, a phenolic coupling protein that has earlier been suggested to participate in hypericin biosynthesis and binding/transportation. The PR-10 genes showed constitutive but variable expression patterns in different H. perforatum tissues. They were all expressed at relatively high levels in leaves, variably in roots and low levels in stem and reproductive parts of the plant with no association with dark glands. The gene expression was up-regulated in leaves after salicylic acid, abscisic acid and wounding treatments but with variable levels. To study exact location of the gene expression, in situ hybridization of hyp-1 transcripts was performed and the accumulation of the Hyp-1 protein was examined in various tissues. The presence of Hyp-1 protein in H. perforatum tissues mostly paralleled with the mRNA levels. In situ RNA hybridization localized the hyp-1 transcripts predominantly in vascular tissues in root and stem, while in leaf the mRNA levels were high also in mesophyll cells in addition to vasculature. Our results indicate that the studied PR-10 genes are likely to contribute to the defence responses in H. perforatum. Furthermore, despite the location of the hyp-1 transcripts in vasculature, no support for the transportation of the Hyp-1 protein to dark glands was found in the current study. The present results together with earlier data question the role of the hyp-1 as a key gene responsible for the hypericin biosynthesis in dark glands of H. perforatum.

  19. Molecular Cloning and Expression Analysis of hyp-1 Type PR-10 Family Genes in Hypericum perforatum.

    Science.gov (United States)

    Karppinen, Katja; Derzsó, Emese; Jaakola, Laura; Hohtola, Anja

    2016-01-01

    Hypericum perforatum L. is an important medicinal plant for the treatment of depression. The plant contains bioactive hypericins that accumulate in dark glands present especially in reproductive parts of the plant. In this study, pathogenesis-related class 10 (PR-10) family genes were identified in H. perforatum, including three previously unidentified members with sequence homology to hyp-1, a phenolic coupling protein that has earlier been suggested to participate in biosynthesis and binding/transportation of hypericin. The PR-10 genes showed constitutive but variable expression patterns in different H. perforatum tissues. They were all expressed at relatively high levels in leaves, variably in roots and low levels in stem and reproductive parts of the plant with no specific association with dark glands. The gene expression was up-regulated in leaves after salicylic acid, abscisic acid and wounding treatments but with variable levels. To study exact location of the gene expression, in situ hybridization of hyp-1 transcripts was performed and the accumulation of the Hyp-1 protein was examined in various tissues. The presence of Hyp-1 protein in H. perforatum tissues mostly paralleled with the mRNA levels. In situ RNA hybridization localized the hyp-1 transcripts predominantly in vascular tissues in root and stem, while in leaf the mRNA levels were high also in mesophyll cells in addition to vasculature. Our results indicate that the studied PR-10 genes are likely to contribute to the defense responses in H. perforatum. Furthermore, despite the location of the hyp-1 transcripts in vasculature, no support for the transportation of the Hyp-1 protein to dark glands was found in the current study. The present results together with earlier data question the role of the hyp-1 as a key gene responsible for the hypericin biosynthesis in dark glands of H. perforatum. PMID:27148343

  20. On the way to measuring gbar with AEgIS

    CERN Document Server

    Palchoudhuri, Paroma

    2015-01-01

    The main goal of AEgIS is to measure the fall of antihydrogen in the Earth's gravitational eld, which is a direct test of Einstein's Weak Equivalence Principle. This gravity measurement requires the production of a pulsed and cold antihydrogen beam whose vertical de ection will be observed in a set of moire de ectometer gratings. The antihydrogen detector was previously built in order to measure the produc- tion and temperature of the antihydrogen atoms and for establishing the formation of the antihydrogen beam. This detector consist of scintillating bers that are con- nected to Multi-Pixel-Photon-Counters (MPPCs) which are further connected to readout electrons. For a total of 816 MPPCs, the bias voltage was tested. It was observed that one channel out of the 816 was not functional. Additionally, in the current setup antiprotons, which are used to produce anti- hydrogen, are cooled to 4 K using electrons. A uorescence spectroscopy technique that uses C 2 molecules to more efficiently cool antiprotons i...

  1. PR10 expression in maize and its effect on host resistance against Aspergillus flavus infection and aflatoxin production.

    Science.gov (United States)

    Chen, Zhi-Yuan; Brown, Robert L; Damann, Kenneth E; Cleveland, Thomas E

    2010-01-01

    Maize (Zea mays L.) is a major crop susceptible to Aspergillus flavus infection and subsequent contamination with aflatoxins, the potent carcinogenic secondary metabolites of the fungus. Protein profiles of maize genotypes resistant and susceptible to A. flavus infection and/or aflatoxin contamination have been compared, and several resistance-associated proteins have been found, including a pathogenesis-related protein 10 (PR10). In this study, RNA interference (RNAi) gene silencing technology was employed to further investigate the importance of PR10. An RNAi gene silencing vector was constructed and introduced into immature Hi II maize embryos through both bombardment and Agrobacterium infection procedures. PR10 expression was reduced by 65% to more than 99% in transgenic callus lines from bombardment. The RNAi-silenced callus lines also showed increased sensitivity to heat stress treatment. A similar reduction in PR10 transcript levels was observed in seedling leaf and root tissues developed from transgenic kernels. When inoculated with A. flavus, RNAi-silenced mature kernels produced from Agrobacterium-mediated transformation showed a significant increase in fungal colonization and aflatoxin production in 10 and six, respectively, of 11 RNAi lines compared with the non-silenced control. Further proteomic analysis of RNAi-silenced kernels revealed a significant reduction in PR10 production in eight of 11 RNAi lines that showed positive for transformation. A significant negative correlation between PR10 expression at either transcript or protein level and kernel aflatoxin production was observed. The results indicate a major role for PR10 expression in maize aflatoxin resistance. PMID:20078777

  2. Molecular cloning of a pathogen/wound-inducible PR10 promoter from Pinus monticola and characterization in transgenic Arabidopsis plants.

    Science.gov (United States)

    Liu, Jun-Jun; Ekramoddoullah, Abul K M; Piggott, Nina; Zamani, Arezoo

    2005-05-01

    In Pinus monticola (Dougl. ex D. Don), the class ten pathogenesis-related (PR10) proteins comprise a family of multiple members differentially expressed upon pathogen infection and other environmental stresses. One of them, PmPR10-1.13, is studied here by investigating its transcriptional regulation in transgenic Arabidopsis plants. For functional analyses of the PmPR10-1.13 promoter, a 1,316-bp promoter fragment and three 5' deletions were translationally fused to the ss-glucuronidase (GUS) reporter gene. The 1,316-bp promoter-driven GUS activity first appeared in hypocotyls and cotyledons in 2- to 3-day-old seedlings. As transgenic plants grew, GUS activity was detected strongly in apical meristems, next in stems and leaves. No GUS activity was detected in roots and in reproductive tissues of flower organs. In adult plants, the PmPR10-1.13 promoter-directed GUS expression was upregulated following pathogen infection and by wounding treatment, which generally mimic the endogenous expression pattern in western white pine. Promoter analysis of 5' deletions demonstrated that two regions between -1,316 and -930, and between -309 and -100 were responsible for the wound responsiveness. By structural and functional comparisons with PmPR10-1.14 promoter, putative wound-responsive elements were potentially identified in the PmPR10-1.13 promoter. In conclusion, PmPR10-1.13 showed properties of a defence-responsive gene, being transcriptionally upregulated upon biotic and abiotic stresses. PMID:15609047

  3. A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible.

    Science.gov (United States)

    Liu, Xiaojun; Huang, Beibei; Lin, Juan; Fei, Jiong; Chen, Zhonghai; Pang, Yongzhen; Sun, Xiaofen; Tang, Kexuan

    2006-03-01

    A cDNA clone (designated as SsPR10, GenBank Accession Number AY660753 ) encoding a PR10 protein from yellow-fruit nightshade (Solanum surattense) was isolated and characterized. SsPR10 encoded a 160-amino-acid polypeptide with a predicted molecular mass of 17.58 kDa and pI of 5.29. Sequence alignments showed that SsPR10 had high identity (68.1%) with CaPR10, but had only about 31.7% identity with JIOsPR10 at the amino acid level. Genomic DNA gel blot analysis indicated that SsPR10 belonged to a multigene family. The constitutively expressed SsPR10 was detected to be the highest in roots of the sterile seedlings cultured in jars, while SsPR10 expression was the highest in old yellow leaves from the seedlings incubated with sap containing TMV. SsPR10 always expressed at slightly higher level in senescent leaves than in tender ones under both conditions. Further expression analysis revealed that the signaling components of defense/stress pathways (MeJA, SA, ABA, GA3, H2O2 and Cu2+) up-regulated significantly the SsPR10 mRNA levels over the control. However, darkness failed to induce SsPR10 expression and its expression was also inhibited by cold treatment. The SsPR10 was successfully expressed in Eschericha coli and the expressed protein was purified to near homogeneity. The dialytically renatured SsPR10 protein without phosphorylation exhibited ribonucleolytic activity against S. surattense leaf total RNA preparations and could inhibit hyphal growth of Pyricularia oryzae. Our findings suggest that the novel stress- and pathogen-inducible SsPR10 with ribonucleolytic and antimicrobial activity participates not only in the defense/stress response pathways but also in plants' growth, development and senescence. PMID:16473659

  4. Improved shoot regeneration, salinity tolerance and reduced fungal susceptibility in transgenic tobacco constitutively expressing PR-10a gene

    Directory of Open Access Journals (Sweden)

    Parinita eAgarwal

    2016-02-01

    Full Text Available Plants in ecosystems are simultaneously exposed to abiotic and biotic stresses, which restrict plant growth and development. The complex responses to these stresses are largely regulated by plant hormones, which in turn, orchestrate the different biochemical and molecular pathways to manoeuvre stress tolerance. The PR-10 protein family is reported to be involved in defence regulation, stress response and plant growth and development. The JcPR-10a overexpression resulted in increased number of shoot buds in tobacco (Nicotiana tabacum, which could be due to high cytokinin to auxin ratio in the transgenics. The docking analysis shows the binding of three BAP molecules at the active sites of JcPR-10a protein. JcPR-10a transgenics showed enhanced salt tolerance, as was evident by increased germination rate, shoot and root length, relative water content, proline, soluble sugar and amino acid content under salinity. Interestingly, the transgenics also showed enhanced endogenous cytokinin level as compared to WT, which, further increased with salinity. Exposure of gradual salinity resulted in increased stomatal conductance, water use efficiency, photosynthesis rate and reduced transpiration rate. Furthermore, the transgenics also showed enhanced resistance against Macrophomina fungus. Thus, JcPR-10a might be working in co-ordination with cytokinin signalling in mitigating the stress induced damage by regulating different stress signalling pathways, leading to enhanced stress tolerance.

  5. Improved Shoot Regeneration, Salinity Tolerance and Reduced Fungal Susceptibility in Transgenic Tobacco Constitutively Expressing PR-10a Gene.

    Science.gov (United States)

    Agarwal, Parinita; Dabi, Mitali; More, Prashant; Patel, Khantika; Jana, Kalyanashis; Agarwal, Pradeep K

    2016-01-01

    Plants in ecosystems are simultaneously exposed to abiotic and biotic stresses, which restrict plant growth and development. The complex responses to these stresses are largely regulated by plant hormones, which in turn, orchestrate the different biochemical and molecular pathways to maneuver stress tolerance. The PR-10 protein family is reported to be involved in defense regulation, stress response and plant growth and development. The JcPR-10a overexpression resulted in increased number of shoot buds in tobacco (Nicotiana tabacum), which could be due to high cytokinin to auxin ratio in the transgenics. The docking analysis shows the binding of three BAP molecules at the active sites of JcPR-10a protein. JcPR-10a transgenics showed enhanced salt tolerance, as was evident by increased germination rate, shoot and root length, relative water content, proline, soluble sugar and amino acid content under salinity. Interestingly, the transgenics also showed enhanced endogenous cytokinin level as compared to WT, which, further increased with salinity. Exposure of gradual salinity resulted in increased stomatal conductance, water use efficiency, photosynthesis rate and reduced transpiration rate. Furthermore, the transgenics also showed enhanced resistance against Macrophomina fungus. Thus, JcPR-10a might be working in co-ordination with cytokinin signaling in mitigating the stress induced damage by regulating different stress signaling pathways, leading to enhanced stress tolerance. PMID:26973666

  6. Pojehali, hüüab "Jääaeg 2" / Tiit Tuumalu

    Index Scriptorium Estoniae

    Tuumalu, Tiit, 1971-

    2006-01-01

    Täispikk animafilm "Jääaeg 2 : Suur sula" ("Ice Age 2 : The Meltdown") : režissöör Carlos Sandanha : eestikeelse dublaazhi režissöör Peeter Simm : Ameerika Ühendriigid 2006. Peeter Simm dublaazhi tegemisest

  7. "Jääaeg 2" : tiiger Diego kurvastas Marko Matveret / Jaanus Kulli

    Index Scriptorium Estoniae

    Kulli, Jaanus, 1955-

    2006-01-01

    Täispikk animafilm "Jääaeg 2 : Suur sula" ("Ice Age 2 : The Meltdown") : režissöör Carlos Sandanha : eestikeelse dublaazhi režissöör Peeter Simm : Ameerika Ühendriigid 2006. Filmi dubleerimisest dubleerijate kommentaaridega

  8. Astrocyte Elevated Gene-1 (AEG-1) Contributes to Non-thyroidal Illness Syndrome (NTIS) Associated with Hepatocellular Carcinoma (HCC)*

    OpenAIRE

    Srivastava, Jyoti; Robertson, Chadia L.; Gredler, Rachel; Siddiq, Ayesha; Rajasekaran, Devaraja; Akiel, Maaged A; Emdad, Luni; Mas, Valeria; Mukhopadhyay, Nitai D.; FISHER, PAUL B.; Sarkar, Devanand

    2015-01-01

    Background: Astrocyte elevated gene-1 (AEG-1) inhibits retinoid X receptor (RXR) function and is overexpressed in human hepatocellular carcinoma (HCC), which is associated with non-thyroidal illness syndrome (NTIS).

  9. Cloning and Analysis of Promoter-like Sequence of ARAhPR10 Gene from Peanut (Arachis hypogaea L.)%花生ARAhPR10基因启动子序列的克隆及分析

    Institute of Scientific and Technical Information of China (English)

    温世杰; 谢纯政; 李玲; 刘海燕; 梁炫强

    2012-01-01

    PR10 (pathogenesis-related class 10 protein)类蛋白与植物的抵御外来病害及系统获得性抗性(SAR)有着紧密联系,本文采用基于PCR的基因组DNA步移法,从抗黄曲霉花生品种粤油20中克隆ARAhPR10 (Aspergillus flavus-resistant AhPR10)基因起始密码子ATG上游256 bp类似启动子序列,并对其进行植物顺式作用元件数据库PLACE预测分析.结果表明,该类似启动子序列含有4处TATA box和2处CAAT box保守的启动子结构元件,还有6处W-box、1处BIHD1和3处GT-1 motif抗逆应答元件,其中W-box常见于PR蛋白的启动子区内参与病程应答.我们初步认为本研究克隆的序列可能是ARAhPR10基因的启动子.%PR10 (pathogenesis-related class 10 protein) is considered as a kind of proteins that close associated with the defense of plant diseases and system acquired resistance (SAR). The 256 bp promoter-like sequence of 5' upstream of initial codon of ARAhPR10 gene from A spergillus flav us -resistant Arochis hypogaea L. Cv. Yueyou20 was cloned by using PCR-based genomic DNA walking approach. The prediction analysis of this promoter-like sequence was carried out based on plant cis-acting element database, PLACE. The results showed that the promoter-like sequence contained conservative structural elements of four TATA boxes and two CAAT boxes, and the promoter response element motifs of six W-boxes, one site of BIHD1 and three sites of GT-1, where W-box usually involved in the PR protein response of promoter region of PR proteins. Our preliminary results indicated that the cloned sequences of this study might be the promoter of A RA hPR10 gene.

  10. Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen

    Directory of Open Access Journals (Sweden)

    van't Westende Wendy PC

    2009-03-01

    Full Text Available Abstract Background Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch (Betula pendula have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus Betula. Q-TOF LC-MSE was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome. Results All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01 were abundant in all species except B. lenta, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01 was only found in B. pendula and its closest relatives. Conclusion Q-TOF LC-MSE allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. B. pendula contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of B. lenta, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.

  11. AEG-1与CXCR4对乳腺癌脑转移的影响%Effect of AEG-1 and CXCR4 Gene on Breast Cancer Brain Metastasis

    Institute of Scientific and Technical Information of China (English)

    曲明阳; 李森; 赵胜男; 邢光明

    2011-01-01

    目的 探讨AEG-1与CXCR4基因表达对乳腺癌脑转移的影响.方法 对1997~2007年收治的乳腺癌患者进行随访,以发生脑转移的33例患者作为病例组,以未发生脑转移的45例患者作为对照组.通过免疫组化法,对照分析AEG-1及CXCR4对脑转移的影响.结果 脑转移组中AEG-1及CXCR4的阳性表达率分别为63.6%和60.6%,与其在对照组中表达(31.1%和33.3%)差异显著(P<0.05).logistic回归分析结果显示,AEG-1和CXCR4回归系数分别为1.242和1.545.结论 AEG-1和CXCR4阳性表达是乳腺癌发生脑转移的独立危险因子,AEG-1及CXCR4有望成为针对乳腺癌脑转移的高特异性早期诊断指标及基因治疗靶点.%Objective Explore the effect of AEG-1 and CXCR4 gene on brain metastasis in breast cancer. Methods 33 breast cancer patients with brain metastasis and 45 patients without brain metastasis in our hospital from 1997 to 2007 were chosen randomly for the case control study by immunohisto chemical method. Results the expression rate for AEG-land CXCR4 in the two groups were 63. 6% ,60. 6% and 31. 1% ,33. 3% respectively,with a notebly difference be observed, the regression coefficient for AEG-1 and CXCR4 was 1. 242 and 1. 545. Conclusion AEG-1 and CXCR4 were independent risk factors and may be specific diagnosis index and gene treatment taget for brain metastasis in breast cancer.

  12. Expression of AEG-1 mRNA and protein in colorectal cancer patients and colon cancer cell lines

    Directory of Open Access Journals (Sweden)

    Gnosa Sebastian

    2012-05-01

    Full Text Available Abstract Background Astrocyte elevated gene 1 (AEG-1, an important oncogene, has been shown to be overexpressed in several types of cancers. In colorectal cancer (CRC, the protein level of AEG-1 is up-regulated in tumour tissue compared to normal mucosa, showing prognostic significance. Since little is known about the transcriptional level of AEG-1 expression and its biological pathway in CRC the aim of the present study was to examine the relationship of AEG-1 mRNA expression, the protein level and clinicopathological variables as well as its biology pathway in CRC. Material and methods The mRNA expression of AEG-1 was analysed by qPCR in fresh frozen patient samples including 156 primary tumours, along with the corresponding normal mucosa, and in five colon cancer cell lines, SW480, SW620, KM12C, KM12SM and KM12L4a. AEG-1 protein expression was investigated by immunohistochemistry in paraffin-embedded materials from 74 distant normal mucosa, 107 adjacent mucosa, 158 primary tumour, 35 lymph node metastasis and 9 liver metastasis samples. In addition, the AEG-1 protein expression was elucidated in the cell lines by Western blot. Results The lymph node metastatic cell line SW620 had a significantly higher AEG-1 mRNA (0.27 ± 0.02 expression compared to the primary tumour cell line SW480 (0.17 ± 0.04, p = 0.026. AEG-1 expression at the mRNA level and/or the protein level was significantly up-regulated gradually from normal mucosa to primary CRC, and then to lymph node metastasis and finally to liver metastasis (p p = 0.047, as well as mRNA and protein expression with the tumour stage (p p  Conclusion AEG-1 is up-regulated, at the mRNA and the protein level, during CRC development and aggressiveness, and is related to tumour location and stage. It may play its role in CRC through the NF-κB signaling pathway.

  13. 抗人AEG-1单克隆抗体可变区基因克隆及序列分析%Developing an Anti-human AEG-1 Monoclonal Antibody Secreting Hybridoma and Its Variable Region Fragment Amplification

    Institute of Scientific and Technical Information of China (English)

    龙敏; 董柯; 王希; 陈曦; 刘冲; 刘丽; 张惠中

    2014-01-01

    AEG-1基因位于人染色体8q22,编码582个氨基酸,参与多种信号转导途径并与多种恶性肿瘤的发生、发展及生物学表型密切相关.为更好地探讨AEG-1生物学功能,以纯化的pGSTag-AEG-1蛋白免疫BALB/c小鼠,应用细胞融合技术并经筛选及鉴定,获得了分泌抗人AEG-1单克隆抗体的杂交瘤细胞株1E3;Western blot及免疫组化证实该细胞株分泌的单克隆抗体能与肿瘤细胞中AEG-1蛋白特异性结合;RT-PCR方法从1E3细胞中克隆出抗AEG-1抗体的VH和VL基因片段,通过测序分析、碱基和蛋白序列的比对确认该株抗体为鼠源性IgG的轻、重链可变区基因.进一步运用Kabat System在线分析系统对VH和VL基因进行结构分析,确证FWRs和CDRs的结构完整,VH编码117个氨基酸;VL编码119个氨基酸,属于轻链κV家族.实验结果为进一步研究AEG-1与恶性肿瘤发生、发展的关系及在其临床诊断中的应用奠定了基础.

  14. Development of Ultra Low-Temperature Electronics for the AEgIS Experiment

    CERN Document Server

    Kaltenbacher, Thomas; Kellerbauer, Alban; Doser, Michael; Caspers, Friedhelm

    This thesis presents the development of electronics for operation at cryogenic temperatures, with particular emphasis on the cryogenic electronics required for the Antimatter Experiment: Gravity, Interferometry, Spectroscopy (AEgIS) experiment at the European Organisation for Nuclear Research (CERN). The research is focused on a highly sensitive charged particle detection system for a Penning trap, on cryogenic low-pass filters and on a low-loss DC-contact RF switch. The detection system consists of a high quality factor tuned circuit including a superconducting coil, and a low-noise amplifier. Since the experimental setup of the AEgIS experiment requires it, the developed electronics must reliably operate at 4.2 K (~269C) and in high constant magnetic field of more than 1 Tesla. Therefore, the performance of the cryogenic electronic designs were carefully evaluated at low-temperature/high magnetic field, the result of which have important implications for the AEgIS experiment. Moreover, a new possibility of ...

  15. An IgE epitope of Bet v 1 and fagales PR10 proteins as defined by a human monoclonal IgE

    DEFF Research Database (Denmark)

    Hecker, J.; Diethers, A.; Schulz, D.;

    2012-01-01

    formats including IgE. Using variants of Bet v 1, the epitope of the antibody was mapped and extrapolated to other PR10 proteins. RESULTS: The obtained antibodies exhibited pronounced reactivity with Bet v 1, but were not reactive with the homologous PR10 protein Mal d 1. The epitope as defined by the Ig......-reactivities predicted by primary structure analyses of different isoforms and PR10 proteins were verified by allergen chip-based analyses. CONCLUSIONS: The obtained results demonstrate that hybrid IgE repertoires represent a source for human antibodies with genuine paratopes. The IgE-derived information about the Ig......E epitope nature of Bet v 1 and homologues allows for detailed insights into molecular aspects of allergenicity and cross-reactivity within the PR10 protein family....

  16. 2 ühe hoobiga. "Uskumatu Hulk", "Aeg on käes..." / Helmut Jänes

    Index Scriptorium Estoniae

    Jänes, Helmut, 1972-

    2008-01-01

    Seiklusfilm "Uskumatu Hulk" ("The Incredible Hulk") : režissöör Louis Leterrier : Ameerika Ühendriigid 2008 ja ulmefilm "Aeg on käes..." ("The Happening") : režissöör M. Night Shyamalan : peaosas Mark Wahlberg : Ameerika Ühendriigid - India 2008

  17. Development of the Cryogenic System of AEgIS at CERN

    CERN Document Server

    Derking, J H; Burghart, G; Doser, M; Dudarev, A; Haider, S

    2014-01-01

    The AEgIS (Antimatter Experiment: Gravity, Interferometry, Spectroscopy) experiment is located at the antiproton decelerator complex of CERN. The main goal of the experiment is to perform the first direct measurement of the Earth’s gravitational acceleration on antihydrogen atoms within 1% precision. The antihydrogen is produced in a cylindrical Penning trap by combining antiprotons with positrons. To reach the precision of 1%, the antihydrogen has to be cooled to 100 mK to reduce its random velocity. A dilution refrigerator is selected to deliver the necessary cooling capacity of 100 μW at 50 mK. The AEgIS cryogenic system basically consists of cryostats for a 1-T and for a 5-T superconducting magnet, a central region cryostat, a dilution refrigerator cryostat and a measurement cryostat with a Moiré deflectometer to measure the gravitational acceleration. In autumn 2012, the 1-T cryostat, 5-T cryostat and central region cryostat were assembled and commissioned. The apparatus is cooled down in eight days ...

  18. Trigermanides AEGe{sub 3} (AE = Ca, Sr, Ba). Chemical bonding and superconductivity

    Energy Technology Data Exchange (ETDEWEB)

    Castillo, Rodrigo; Schnelle, Walter; Baranov, Alexey I.; Burkhardt, Ulrich; Bobnar, Matej; Cardoso-Gil, Raul; Schwarz, Ulrich; Grin, Yuri [Max-Planck-Institut fuer Chemische Physik Fester Stoffe, Dresden (Germany)

    2016-08-01

    The crystal structures of the trigermanides AEGe{sub 3}(tI32) (AE = Ca, Sr, Ba; space group I4/mmm, for SrGe{sub 3}: a = 7.7873(1), c = 12.0622(3) Aa) comprise Ge{sub 2} dumbbells forming layered Ge substructures which enclose embedded AE atoms. The chemical bonding analysis by application of the electron localizability approach reveals a substantial charge transfer from the AE atoms to the germanium substructure. The bonding within the dumbbells is of the covalent two-center type. A detailed analysis of SrGe{sub 3} reveals that the interaction on the bond-opposite side of the Ge{sub 2} groups is not lone pair-like - as it would be expected from the Zintl-like interpretation of the crystal structure with anionic Ge layers separated by alkaline-earth cations - but multi-center strongly polar between the Ge{sub 2} dumbbells and the adjacent metal atoms. Similar atomic interactions are present in CaGe{sub 3} and BaGe{sub 3}. The variation of the alkaline-earth metal has a merely insignificant influence on the superconducting transition temperatures in the s,p-electron compounds AEGe{sub 3}.

  19. Combination of nanoparticle-delivered siRNA for Astrocyte elevated gene-1 (AEG-1) and all-trans retinoic acid (ATRA): an effective therapeutic strategy for hepatocellular carcinoma (HCC)

    Science.gov (United States)

    Rajasekaran, Devaraja; Srivastava, Jyoti; Ebeid, Kareem; Gredler, Rachel; Akiel, Maaged; Jariwala, Nidhi; Robertson, Chadia L.; Shen, Xue-Ning; Siddiq, Ayesha; Fisher, Paul B.; Salem, Aliasger K.; Sarkar, Devanand

    2016-01-01

    Hepatocellular carcinoma (HCC) is a fatal cancer with no effective therapy. Astrocyte elevated gene-1 (AEG-1) plays a pivotal role in hepatocarcinogenesis and inhibits retinoic acid-induced gene expression and cell death. Combination of a lentivirus expressing AEG-1 shRNA and all-trans retinoic acid (ATRA) profoundly and synergistically inhibited subcutaneous human HCC xenografts in nude mice. We now have developed liver-targeted nanoplexes by conjugating poly(amidoamine) (PAMAM) dendrimers with polyethylene glycol (PEG) and lactobionic acid (Gal) (PAMAM-PEG-Gal) which were complexed with AEG-1 siRNA (PAMAM-AEG-1si). The polymer conjugate was characterized by 1H-NMR, MALDI and mass spectrometry, and optimal nanoplex formulations were characterized for surface charge, size and morphology. Orthotopic xenografts of human HCC cell QGY-7703 expressing luciferase (QGY-luc) were established in the livers of athymic nude mice and tumor development was monitored by bioluminescence imaging (BLI). Tumor-bearing mice were treated with PAMAM-siCon, PAMAM-siCon+ATRA, PAMAM-AEG-1si and PAMAM-AEG-1si+ATRA. In the control group the tumor developed aggressively. ATRA showed little effect due to high AEG-1 levels in QGY-luc cells. PAMAM-AEG-1si showed significant reduction in tumor growth and the combination of PAMAM-AEG-1si+ATRA showed profound and synergistic inhibition so that the tumors were almost undetectable by BLI. A marked decrease in AEG-1 level was observed in tumor samples treated with PAMAM-AEG-1si. The group treated with PAMAM-AEG-1si+ATRA nanoplexes showed increased necrosis, inhibition of proliferation and increased apoptosis when compared to other groups. Liver is an ideal organ for RNAi therapy and ATRA is an approved anti-cancer agent. Our exciting observations suggest that the combinatorial approach might be an effective way to combat HCC. PMID:26079152

  20. Multiple Patterns of Regulation and Overexpression of a Ribonuclease-Like Pathogenesis-Related Protein Gene, OsPR10a, Conferring Disease Resistance in Rice and Arabidopsis

    Science.gov (United States)

    He, Siou-Luan; Chen, Jyh-Lang; Jiang, Jian-Zhi; Chen, Bo-Hong; Hou, Yi-Syuan; Chen, Ruey-Shyang; Hong, Chwan-Yang; Ho, Shin-Lon

    2016-01-01

    An abundant 17 kDa RNase, encoded by OsPR10a (also known as PBZ1), was purified from Pi-starved rice suspension-cultured cells. Biochemical analysis showed that the range of optimal temperature for its RNase activity was 40–70°C and the optimum pH was 5.0. Disulfide bond formation and divalent metal ion Mg2+ were required for the RNase activity. The expression of OsPR10a::GUS in transgenic rice was induced upon phosphate (Pi) starvation, wounding, infection by the pathogen Xanthomonas oryzae pv. oryzae (Xoo), leaf senescence, anther, style, the style-ovary junction, germinating embryo and shoot. We also provide first evidence in whole-plant system, demonstrated that OsPR10a-overexpressing in rice and Arabidopsis conferred significant level of enhanced resistance to infection by the pathogen Xoo and Xanthomona campestris pv. campestris (Xcc), respectively. Transgenic rice and Arabidopsis overexpressing OsPR10a significantly increased the length of primary root under phosphate deficiency (-Pi) condition. These results showed that OsPR10a might play multiple roles in phosphate recycling in phosphate-starved cells and senescing leaves, and could improve resistance to pathogen infection and/or against chewing insect pests. It is possible that Pi acquisition or homeostasis is associated with plant disease resistance. Our findings suggest that gene regulation of OsPR10a could act as a good model system to unravel the mechanisms behind the correlation between Pi starvation and plant-pathogen interactions, and also provides a potential application in crops disease resistance. PMID:27258121

  1. Development of nuclear emulsions with 1μm spatial resolution for the AEgIS experiment

    International Nuclear Information System (INIS)

    The main goal of the AEgIS experiment at CERN is to test the weak equivalence principle for antimatter. We will measure the Earth's gravitational acceleration g¯ with antihydrogen atoms being launched in a horizontal vacuum tube and traversing a moiré deflectometer. We intend to use a position sensitive device made of nuclear emulsions (combined with a time-of-flight detector such as silicon μ-strips) to measure precisely their annihilation points at the end of the tube. The goal is to determine g¯ with a 1% relative accuracy. In 2012 we tested emulsion films in vacuum and at room temperature with low energy antiprotons from the CERN antiproton decelerator. First results on the expected performance for AEgIS are presented

  2. Development of nuclear emulsions with 1 $\\mu$m spatial resolution for the AEgIS experiment

    CERN Document Server

    Kimura, M; Ahlén, O; Amsler, C; Ariga, A; Ariga, T; Belov, A.S; Bonomi, G; Bräunig, P; Bremer, J; Brusa, R.S; Burghart, G; Cabaret, L; Canali, C; Caravita, R; Castelli, F; Cerchiari, G; Cialdi, S; Comparat, D; Consolati, G; Di Domizio, S; Di Noto, L; Doser, M; Dudarev, A; Ereditato, A; Ferragut, R; Fontana, A; Genova, P; Giammarchi, M; Gligorova, A; Gninenko, S.N; Haider, S; Hogan, S.D; Huse, T; Jordan, E; Jørgensen, L.V; Kaltenbacher, T; Kawada, J; Kellerbauer, A; Knecht, A; Krasnický, D; Lagomarsino, V; Mariazzi, S; Matveev, V.A; Merkt, F; Moia, F; Nebbia, G; Nédélec, P; Oberthaler, M.K; Pacifico, N; Petrácek, V; Pistillo, C; Prelz, F; Prevedelli, M; Regenfus, C; Riccardi, C; Røhne, O; Rotondi, A; Sandaker, H; Scampoli, P; Spacek, M; Storey, J; Subieta Vasquez, M.A; Testera, G; Trezzi, D; Vaccarone, R; Zavatarelli, S

    2013-01-01

    The main goal of the AEgIS experiment at CERN is to test the weak equivalence principle for antimatter. We will measure the Earth ' s gravitational acceleration g with antihydrogen atoms being launched in a horizontal vacuum tube and traversing a moiré de fl ectometer. We intend to use a position sensitive device made of nuclear emulsions (combined with a time-of- fl ight detector such as silicon μ strips) to measure precisely their annihilation points at the end of the tube. The goal is to determine g with a 1% relative accuracy. In 2012 we tested emulsion fi lms in vacuum and at room temperature with low energy antiprotons from the CERN antiproton decelerator. First results on the expected performance for AEgIS are presented

  3. Development of nuclear emulsions with 1μm spatial resolution for the AEgIS experiment

    Energy Technology Data Exchange (ETDEWEB)

    Kimura, M. [Albert Einstein Center for Fundamental Physics, Laboratory for High Energy Physics, University of Bern, 3012 Bern (Switzerland); Aghion, S. [Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Istituto Nazionale di Fisica Nucleare, Sez. di Milano, Via Celoria 16, 20133 Milano (Italy); Ahlén, O. [European Organisation for Nuclear Research, Physics Department, 1211 Geneva 23 (Switzerland); Amsler, C., E-mail: claude.amsler@cern.ch [Albert Einstein Center for Fundamental Physics, Laboratory for High Energy Physics, University of Bern, 3012 Bern (Switzerland); Ariga, A.; Ariga, T. [Albert Einstein Center for Fundamental Physics, Laboratory for High Energy Physics, University of Bern, 3012 Bern (Switzerland); Belov, A.S. [Institute for Nuclear Research of the Russian Academy of Sciences, Moscow 117312 (Russian Federation); Bonomi, G. [University of Brescia, Department of Mechanical and Industrial Engineering, Via Branze 38, 25133 Brescia (Italy); Istituto Nazionale di Fisica Nucleare, Sez. di Pavia, Via Agostino Bassi 6, 27100 Pavia (Italy); Bräunig, P. [University of Heidelberg, Kirchhoff Institute for Physics, Im Neuenheimer Feld 227, 69120 Heidelberg (Germany); Bremer, J. [European Organisation for Nuclear Research, Physics Department, 1211 Geneva 23 (Switzerland); Brusa, R.S. [Dipartimento di Fisica, Università di Trento and INFN, Gruppo Collegato di Trento, Via Sommarive 14, 38050 Povo, Trento (Italy); Burghart, G. [European Organisation for Nuclear Research, Physics Department, 1211 Geneva 23 (Switzerland); Cabaret, L. [Laboratoire Aimé Cotton, CNRS, Université Paris Sud, ENS Cachan, Bâtiment 505, Campus d' Orsay, 91405 Orsay Cedex (France); Canali, C. [University of Zurich, Physics Institute, Winterthurerstrasse 190, 8057 Zurich (Switzerland); and others

    2013-12-21

    The main goal of the AEgIS experiment at CERN is to test the weak equivalence principle for antimatter. We will measure the Earth's gravitational acceleration g{sup ¯} with antihydrogen atoms being launched in a horizontal vacuum tube and traversing a moiré deflectometer. We intend to use a position sensitive device made of nuclear emulsions (combined with a time-of-flight detector such as silicon μ-strips) to measure precisely their annihilation points at the end of the tube. The goal is to determine g{sup ¯} with a 1% relative accuracy. In 2012 we tested emulsion films in vacuum and at room temperature with low energy antiprotons from the CERN antiproton decelerator. First results on the expected performance for AEgIS are presented.

  4. Euroopa Inimõiguste konventsiooni artikli 5 3. lõige : mõistlik aeg Euroopa Inimõiguste Kohtu praktikas ja Eesti õiguskorras : [bakalaureusetöö] / Toomas Ventsli ; Tartu Ülikool, õigusteaduskond ; juhendaja: Jaan Ginter

    Index Scriptorium Estoniae

    Ventsli, Toomas

    2003-01-01

    Vabaduse võtmise kohtulik kontroll, kinnipidamise mõistlikkuse hindamine, mõistlik aeg Eesti õigussüsteemis - vahi alla võtmist ja vahi all pidamise pikendamist reguleerivad õigusallikad, eelvangistuses viibitud aeg, vahi all pidamise pikendamine, kinnipidamise põhjendatuse hindamine

  5. Prokaryotic expression and purification of anti-AEG-1 single-chain variable antibody%抗 AEG-1单链可变区抗体的原核表达及纯化

    Institute of Scientific and Technical Information of China (English)

    路蔓; 刘昕阳; 龙敏; 王琛; 刘冲; 郜赵伟; 欧阳永日; 陈曦; 张惠中

    2015-01-01

    Objective To construct anti-astrocyte elevated gene-1(AEG-1)single-chain variable antibody (V23)prokaryotic ex-pression vector,and to conduct the protein purification and immunological activity detection.Methods The Primer5 software was applied to design the primers aiming at the gene sequence of the antibody anti-AEG-1 single-chain variable region for constructing the prokaryotic expression plasmid of PRsetC/V23.After the enzyme digestion by the restriction enzyme Pst1 and correct DNA se-quencing,the prokaryotic expression plasmid was led to E.coli BL21 ,the prokaryotic expression engineering strain containing the V23 gene was constructed.After the induction with IPTG,the interest protein was purified by the magnetic beads with the HIS tag,and the content of interest protein was determined by the SDS-PAGE electrophoresis.Western blot and ELISA were adopted to detect the immune activity of the nti-AEG-1 single-chain variable region antibody.Results For the constructed prokaryotic expres-sion plasmid PRsetC/V23,the single enzyme digestion and sequencing analysis displayed that the constructed V23 gene was com-pletely consistent to the designing sequences.After IPTG induction,SDS-PAGE electrophoresis showed an apparent protein band at 31×103 ,the Western blot detection showed a specific AEG-1 response band at 80 ×103 ,the ELISA test showed the positive re-sults.Conclusion The PRsetC/V23 prokaryotic expression plasmid and the V23 prokaryotic expression engineering strain are suc-cessfully constructed,this engineering strain can express anti-AEG-1 single-chain variable region antibody protein,and the protein has good immune activity.%目的:构建抗星形细胞上调基因1(AEG-1)单链可变区抗体(V23)的原核表达载体,并对表达蛋白进行纯化及免疫活性检测。方法应用 Primer5软件设计针对抗 AEG-1单链可变区抗体基因序列的引物,构建 PRsetC/V23原核表达质粒,经限制性内切酶 Pst1酶切以及 DNA

  6. Particle tracking at 4 K: The Fast Annihilation Cryogenic Tracking (FACT) detector for the AEgIS antimatter gravity experiment

    Energy Technology Data Exchange (ETDEWEB)

    Storey, J., E-mail: james.storey@cern.ch [Albert Einstein Center for Fundamental Physics, Laboratory for High Energy Physics, University of Bern, 3012 Bern (Switzerland); Canali, C. [University of Zurich, Physics Institute, Winterthurerstrasse 190, 8057 Zurich (Switzerland); Aghion, S. [Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Istituto Nazionale di Fisica Nucleare, Sez. di Milano, Via Celoria 16, 20133 Milano (Italy); Ahlén, O. [European Organisation for Nuclear Research, Physics Department, 1211 Geneva 23 (Switzerland); Amsler, C.; Ariga, A.; Ariga, T. [Albert Einstein Center for Fundamental Physics, Laboratory for High Energy Physics, University of Bern, 3012 Bern (Switzerland); Belov, A.S. [Institute for Nuclear Research of the Russian Academy of Sciences, Moscow 117312 (Russian Federation); Bonomi, G. [University of Brescia, Department of Mechanical and Industrial Engineering, Via Branze 38, 25133 Brescia (Italy); Istituto Nazionale di Fisica Nucleare, Sez. di Pavia, Via Agostino Bassi 6, 27100 Pavia (Italy); Bräunig, P. [University of Heidelberg, Kirchhoff Institute for Physics, Im Neuenheimer Feld 227, 69120 Heidelberg (Germany); Bremer, J. [European Organisation for Nuclear Research, Physics Department, 1211 Geneva 23 (Switzerland); Brusa, R.S. [Dipartimento di Fisica, Università di Trento and INFN, Gruppo Collegato di Trento, Via Sommarive 14, 38050 Povo, Trento (Italy); Burghart, G. [European Organisation for Nuclear Research, Physics Department, 1211 Geneva 23 (Switzerland); Cabaret, L. [Laboratoire Aimé Cotton, CNRS, Université Paris Sud, ENS Cachan, Bâtiment 505, Campus d' Orsay, 91405 Orsay Cedex (France); Carante, M. [Istituto Nazionale di Fisica Nucleare, Sez. di Pavia, Via Agostino Bassi 6, 27100 Pavia (Italy); Caravita, R. [University of Milano, Department of Physics, Via Celoria 16, 20133 Milano (Italy); and others

    2013-12-21

    The AEgIS experiment is an international collaboration with the main goal of performing the first direct measurement of the Earth's gravitational acceleration on antimatter. Critical to the success of AEgIS is the production of cold antihydrogen (H{sup ¯}) atoms. The FACT detector is used to measure the production and temperature of the H{sup ¯} atoms and for establishing the formation of a H{sup ¯} beam. The operating requirements for this detector are very challenging: it must be able to identify each of the thousand or so annihilations in the 1 ms period of pulsed H{sup ¯} production, operate at 4 K inside a 1 T solenoidal field and not produce more than 10 W of heat. The FACT detector consists of two concentric cylindrical layers of 400 scintillator fibres with a 1 mm diameter and a 0.6 mm pitch. The scintillating fibres are coupled to clear fibres which transport the scintillation light to 800 silicon photomultipliers. Each silicon photomultiplier signal is connected to a linear amplifier and a fast discriminator, the outputs of which are sampled continuously by Field Programmable Gate Arrays (FPGAs). In the course of the developments for the FACT detector we have established the performance of scintillating fibres at 4 K by means of a cosmic-ray tracker operating in a liquid helium cryostat. The FACT detector was installed in the AEgIS apparatus in December 2012 and will be used to study the H{sup ¯} formation when the low energy antiproton physics programs resume at CERN in the Summer of 2014. This paper presents the design requirements and construction methods of the FACT detector and provides the first results of the detector commissioning.

  7. Cloning and Analysis of Promoter-like Sequence of ARAhPR10 Gene from Peanut (Arachis hypogaea L.)%花生ARAhPR10基因启动子序列的克隆及分析

    Institute of Scientific and Technical Information of China (English)

    温世杰; 谢纯政; 李玲; 刘海燕; 梁炫强

    2012-01-01

    PR10(pathogenesis-related class10protein)类蛋白与植物的抵御外来病害及系统获得性抗性(SAR)有着紧密联系,本文采用基于PCR的基因组DNA步移法,从抗黄曲霉花生品种粤油20中克隆ARAhPR10(Aspergillus flavus-resistant AhPR10)基因起始密码子ATG上游256bp类似启动子序列,并对其进行植物顺式作用元件数据库PLACE预测分析。结果表明,该类似启动子序列含有4处TATA box和2处CAAT box保守的启动子结构元件,还有6处W-box、1处BIHD1和3处GT-1motif抗逆应答元件,其中W-box常见于PR蛋白的启动子区内参与病程应答。我们初步认为本研究克隆的序列可能是ARAhPR10基因的启动子。%PR10 (pathogenesis-related class10 protein) is considered as a kind of proteins that close associated with the defense of plant diseases and system acquired resistance (SAR).The 256 bp promoter-like sequence of 5' upstream of initial codon of ARAhPR10 gene from Aspergillus flavus-resistant Arachis hypogaea L.cv.Yueyou20 was cloned by using PCR-based genomic DNA walking approach.The prediction analysis of this promoter-like sequence was carried out based on plant cis-acting element database,PLACE.The results showed that the promoter-like sequence contained conservative structural elements of four TATA boxs and two CAAT boxes,and the promoter response element motifs of six W-boxs,one site of BIHD1 and three sites of GT-1,where W-box usually involved in the PR protein response of promoter region of PR proteins.Our preliminary results indicated that the cloned sequences of this study might be the promoter of ARAhPR10 gene.

  8. [Kari Tarkiainen, Ülle Tarkiainen. Provinsen bortom havet : Estlands svenska historia 1561-1710; Kari Tarkiainen, Ülle Tarkiainen. Meretagune maa : Rootsi aeg Eestis 1561-1710] / Pärtel Piirimäe

    Index Scriptorium Estoniae

    Piirimäe, Pärtel, 1972-

    2015-01-01

    Arvustus: Tarkiainen Kari, Tarkiainen Ülle. Provinsen bortom havet : Estlands svenska historia 1561-1710 Verlag Atlantis. Stockholm 2013; Tarkiainen, Kari, Tarkiainen, Ülle. Meretagune maa : Rootsi aeg Eestis 1561-1710. verlag Varrak Tallinn 2014

  9. IgE recognition patterns of profilin, PR-10, and tropomyosin panallergens tested in 3,113 allergic patients by allergen microarray-based technology.

    Directory of Open Access Journals (Sweden)

    Enrico Scala

    Full Text Available BACKGROUND: IgE recognition of panallergens having highly conserved sequence regions, structure, and function and shared by inhalant and food allergen sources is often observed. METHODS: We evaluated the IgE recognition profile of profilins (Bet v 2, Cyn d 12, Hel a 2, Hev b 8, Mer a 1, Ole e 2, Par j 3, Phl p 12, Pho d 2, PR-10 proteins (Aln g 1, Api g 1, Bet v 1.0101, Bet v 1.0401, Cor a 1, Dau c 1 and Mal d 1.0108 and tropomyosins (Ani s 3, Der p 10, Hel as 1, Pen i 1, Pen m 1, Per a 7 using the Immuno-Solid phase Allergen Chip (ISAC microarray system. The three panallergen groups were well represented among the allergenic molecules immobilized on the ISAC. Moreover, they are distributed in several taxonomical allergenic sources, either close or distant, and have a route of exposure being either inhalation or ingestion. RESULTS: 3,113 individuals (49.9% female were selected on the basis of their reactivity to profilins, PR-10 or tropomyosins. 1,521 (48.8% patients were reactive to profilins (77.6% Mer a 1 IgE(+, 1,420 (45.6% to PR-10 (92.5% Bet v 1 IgE(+ and 632 (20.3% to tropomyosins (68% Der p 10 IgE(+. A significant direct relationship between different representative molecules within each group of panallergens was found. 2,688 patients (86.4% recognized only one out of the three distinct groups of molecules as confirmed also by hierarchical clustering analysis. CONCLUSIONS: Unless exposed to most of the allergens in the same or related allergenic sources, a preferential IgE response to distinct panallergens has been recorded. Allergen microarray IgE testing increases our knowledge of the IgE immune response and related epidemiological features within and between homologous molecules better describing the patients' immunological phenotypes.

  10. 漾濞大泡核桃病程相关蛋白10基因JsPR10-1的克隆与表达分析

    Institute of Scientific and Technical Information of China (English)

    何华; 陈朝银; 韩青; 张南南; 葛锋; 刘迪秋

    2014-01-01

    病程相关蛋白(pathogenesisrelated protein, PR) 10的激活与积累在植物抗逆境胁迫中有非常重要的作用。根据漾濞大泡核桃(Juglans sigillata)编码PR10的EST(expressed sequence tag)序列设计引物,利用快速扩增cDNA末端技术,克隆得到PR10基因的全长cDNA序列,并命名为JsPR10-1。JsPR10-1全长cDNA为776 bp,含有483 bp的开放阅读框、74 bp 5′-非编码区以及219 bp 3′-非编码区,编码含有160个氨基酸的蛋白质。全长基因序列中含有1个124 bp的内含子。JsPR10-1编码的蛋白质与栎树(Quercus suber)、欧洲山毛榉(Fagus sylvatica)以及欧洲板栗(Castanea sativa)的PR10相似性较高,并且在PR10的系统进化树中与双子叶植物聚为一支。qRT-PCR分析结果表明,植物信号分子水杨酸、茉莉酸、乙烯以及过氧化氢处理均可诱导JsPR10-1表达。在接种胶孢炭疽菌(Colletotrichum gloeosporioides)后,JsPR10-1的表达量迅速上升并在接种8 h时达到最大值,表明JsPR10-1参与漾濞大泡核桃对胶孢炭疽菌的防卫反应。本研究为揭示漾濞大泡核桃抗性机制奠定了理论依据。

  11. Muusika : Juubelikontserdid "ERSO 75". Tüür uuel CD-l. Muusikakonverentsid Tallinnas. Highlightsi festivalist "Eduard Tubin ja tema aeg". Hermanni päev / Vardo Rumessen

    Index Scriptorium Estoniae

    Rumessen, Vardo, 1942-2015

    2001-01-01

    ERSO saab detsembris 75. aastaseks ja sel puhul on kavas kontserdid nii Tartus kui Tallinnas. Ansambel Piano Circus on välja antud uue CD, millele annab nime avateos ئ Erkki-Sven Tüüri "Transmission". Eesti Muusikateaduse Selts korraldab 3.XII EMA-s sümpoosioni teemal Beethoveni muusika. 1. ja 2. XII EMA-s toimub konverents "Interpretatsioonipedagoogika probleeme II". Põhjamaade muusikaajakirja Highlights XI numbris ilmus ülevaade rahvusvahelisest muusikafestivalist "Eduard Tubin ja tema aeg". Eesti teatri- ja muusikamuuseumis toimub helilooja, ajakirjaniku, muusika- ja keeleteadlase, dr. Karl August Hermanni päev

  12. Silencing of the metastasis-linked gene, AEG-1, using siRNA-loaded cholamine surface-modified gelatin nanoparticles in the breast carcinoma cell line MCF-7.

    Science.gov (United States)

    Abozeid, Salma M; Hathout, Rania M; Abou-Aisha, Khaled

    2016-09-01

    Cholamine surface-modified gelatin nanoparticles prepared by the double desolvation method using acetone as a dehydrating agent were selected and potentially evaluated as non viral vectors of siRNA targeting a metastatic gene AEG-1 in MCF-7 breast carcinoma cells. The ability of modified gelatin nanoparticle to complex and deliver siRNA for gene silencing was investigated. Hence, Particle size, surface charge (zeta potential) and morphology of siRNA/Gelatin nanoparticles (siGNPs) were characterized via dynamic light scattering (DLS), scanning electron microscopy (SEM) and transmission electron microscope (TEM). Moreover, the nanoparticles cytotoxicity, loading efficiency and interaction with MCF-7 human breast carcinoma cells were evaluated. Cationized GNPs of mean size range of 174nm and PDI of 0.101 were produced. The loading efficiency of siGNPs at a Nitrogen/Phosphate (N/P) ratio (w/w) of 200:1 was approximately 96%. Cellular uptake was evaluated after FITC conjugation where the particles produced high transfection efficiency. Finally, ELISA analysis of AEG-1/MTDH expression demonstrated the gene silencing effect of siGNPs, as more than 75% MTDH protein were inhibited. Our data indicate that cholamine modified GNPs pose a promising non-viral siRNA carrier for altering gene expression in MCF-7 breast cancer cells with many advantages such as relatively high gene transfection efficiency and efficient silencing ability. PMID:27285732

  13. In vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen‐inducible PR 10 promoterIn vitro tolerance to Botrytis cinerea of grapevine 41B rootstock in transgenic plants expressing the stilbene synthase Vst1 gene under the control of a pathogen‐inducible PR 10 promoter

    OpenAIRE

    Coutos-Thévenot, Pierre; Poinssot, Benoît; Bonomelli, A.; Yean, H.; Breda, C; Buffard, D; Esnault, R.; Hain, R; Boulay, M.

    2001-01-01

    Resveratrol is a major phytoalexin in grapevine but its synthesis in response to phytopathogen attack decreases with grape berry ripening, A chimeric gene combining an alfalfa PR 10 promoter and Vst1 (Vitis stilbene synthase 1) gene was introduced into the genome of 41B rootstock, Transgenic plants were analysed for resveratrol production in leaves infected with Botrytis using an in vitro test. Among the 50 transgenic lines analysed, some exhibited a production lower than the non-transgenic c...

  14. High-Content Functional Screening of AEG-1 and AKR1C2 for the Promotion of Metastasis in Liver Cancer.

    Science.gov (United States)

    Li, Cong; Wu, Xia; Zhang, Wei; Li, Jia; Liu, Huawei; Hao, Ming; Wang, Junsong; Zhang, Honghai; Yang, Gengxia; Hao, Meijun; Sheng, Shoupeng; Sun, Yu; Long, Jiang; Li, Juan; Zhuang, Fengfeng; Hu, Caixia; Li, Li; Zheng, Jiasheng

    2016-01-01

    Liver cancer is one of the most lethal cancer types in humans, but our understanding of the molecular mechanisms underlying this process remains insufficient. Here, we conducted high-content screening of the potential genes involved in liver cancer metastasis, which we selected from the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, based on the SAMcell method and RNA interference technology. We identified two powerful genes in the liver cancer metastasis process, AEG-1 and AKR1C2, both of which proved to be positive regulators in promoting metastasis in liver cancer. Further clinical results verified their roles in liver cancer. In summary, these findings could provide new insight into the liver cancer mechanism and potentially therapeutic novel targets for liver cancer therapies in the future.

  15. Positronium in the AEgIS experiment: study on its emission from nanochanneled samples and design of a new apparatus for Rydberg excitations

    CERN Document Server

    Di Noto, Lea

    This experimental thesis has been done in the framework of AEgIS (Antimatter Experiment: Gravity, Interferometry, Spectroscopy), an experiment installed at CERN, whose primary goal is the measurement of the Earth's gravitational acceleration on anti-hydrogen. The antiatoms will be produced by the charge exchange reaction, where a cloud of Ps in Rydberg states interacts with cooled trapped antiprotons. Since the charge exchange cross section depends on Ps velocity and quantum number, the velocity distribution of Ps emitted by a positron-positronium converter as well as its excitation in Rydberg states have to be studied and optimized. In this thesis Ps cooling and emission into vacuum from nanochannelled silicon targets was studied by performing Time of Flight measurements with a dedicated apparatus conceived to receive the slow positron beam as produced at the Trento laboratory or at the NEPOMUC facility at Munich. Measurements were done by varying the positron implantation energy, the sample temperature and ...

  16. Moodne aeg : Moeuudised

    Index Scriptorium Estoniae

    2000-01-01

    Moes on puhvseelik, mis moekriitik Amanda Harlech'i arvates teeb naise keha inetuks. Londonis elava prantslanna Emma Cassid'i cool'id ridikülid. Sian Evansi toidukäruga sõrmused. Graafik James Jarvise disainitud Briti firma Silas'i Evil Martini nimeline kummilelu ning tema sõber kummikass Bubba the Car.

  17. Aeg aias / Jaan Mettik

    Index Scriptorium Estoniae

    Mettik, Jaan

    2007-01-01

    Päikesekellade ajaloost, tüüpidest, Santiago Calatrava kavandatud jalakäijate sillast-päikesekellast Californias, Eesti päikesekelladest Kadrioru pargis, Haapsalu rannapromenaadil (Roman Haavamägi) ja Saue tammepargis (Hanno Grossschmidt, Tomoni Hayashi). Kuukelladest, Karl Linne lillekellast, libalillekelladest. Juhised päikesekella tegemiseks. Lk. 60-61 "Ajanäitajaid lillekellale"

  18. Jõhvist Tallinna / Raivo Aeg

    Index Scriptorium Estoniae

    Aeg, Raivo, 1962-

    2008-01-01

    Ilmunud ka: Postimees : na russkom jazõke 28. jaan. lk. 9. Maanteeameti lepinguliste partnerite teehooldustöödest kirjutades meenutab politseiameti peadirektor, et liiklusõnnetustes hukkus 2007. aastal 196 inimest ja küsib, kumb läheb kallimaks maksma, kas teehoolduseks kulutatav kütus ja suurem lepinguraha või hukkunud inimene. Vastuseks vt. Rain Hallimäe art. 28. jaan. Postimees lk. 17

  19. Inglite aeg / Eve Variksoo

    Index Scriptorium Estoniae

    Variksoo, Eve

    2003-01-01

    Hoff-galeriist, tekstiiliateljeest. Galeriis saab tekstiilikunstnike Tiina Kulli, Einike Soosaare ja Merle Suurkase ning keraamik Tiina Kaljuste juhendamisel valmistada jõulukaunistusi. Savist ingli, vildist lumemehe, nöörikera ja takuingli tegemise õpetus. 9 ill

  20. "Eesti aeg" / Sirje Helme

    Index Scriptorium Estoniae

    Helme, Sirje, 1949-

    2002-01-01

    Välismaal avatakse kolm eesti kaasaegse kunsti näitust : Taxispalais' galeriis Innsbruckis ("The Baltic Times"), Budapestis Ludwigi muuseumis ("Breath") ja Gdanskis Laznia ("Closing the Distance") palees. Galeriide ja muuseumide tutvustus, eksponeeritavate kunstnike osaline loetelu

  1. 漾濞大泡核桃病程相关蛋白10基因JsPR10-1的克隆与表达分析%Cloning and Expression Analysis of a Pathogenesis-related Protein 10 Gene from Juglans sigillata

    Institute of Scientific and Technical Information of China (English)

    何华; 陈朝银; 韩青; 张南南; 葛锋; 刘迪秋

    2014-01-01

    The activation and accumulation of pathogenesis-related protein (PR) 10 play predominant roles in the defense response against pathogens.Based on a Juglans sigillata expressed sequence tag (EST) encoding PR10,gene-specific primers were designed and used to amplify the full-length cDNA of a novel PR10 gene by rapid amplification of cDNA ends (RACE).This gene was named as JsPR10-1.JsPR10-1 was 776 bp in length with an open reading frame (ORF) of 483 bp,a 5'-untranslated region (UTR) of 74 bp,and a 3'-UTR of 219 bp,and the ORF encoded a protein with 160 amino acid residues.There was an intron of 124 bp in the genomic sequence of JsPR10-1.Homology analysis indicated that JsPR10-1 was homologous with PR10s from Quercus suber,Fagus sylvatica and Castanea sativa;moreover,JsPR10-1 clustered together with the PR10s from dicots in the phylogenetic tree.qRT-PCR analysis showed that the expression levels of JsPR10-1 were induced by four different plant signaling molecules,including salicylic acid,jasmonic acid,ethylene,and H2O2.In addition,after inoculation with Colletotrichum gloeosporioides,JsPR10-1 was sharply up-regulated with the highest expression level at 8 h.The JsPR10-1 gene was involved in the defense response against C.gloeosporioides.This experiment lays a theoretical foundation for revealing the mechanism of resistance in J.sigillata.%病程相关蛋白(pathogenesis-related protein,PR) 10的激活与积累在植物抗逆境胁迫中有非常重要的作用.根据漾濞大泡核桃(Juglans sigillata)编码PR10的EST (expressed sequence tag)序列设计引物,利用快速扩增cDNA末端技术,克隆得到PR10基因的全长cDNA序列,并命名为JsPR10-1.JsPR10-1全长cDNA为776 bp,含有483 bp的开放阅读框、74 bp 5'-非编码区以及219 bp 3'-非编码区,编码含有160个氨基酸的蛋白质.全长基因序列中含有1个124 bp的内含子.JsPR10-1编码的蛋白质与栎树(Quercus suber)、欧洲山毛榉(Fagus sylvatica

  2. AEG评审在型号合格审定中的研究与应用%Research and Application of AEG Evaluation on Type Certification

    Institute of Scientific and Technical Information of China (English)

    段本印

    2015-01-01

    概述了申请人的航空器评审工作,并简要对比分析了运行符合性清单、型别等级和训练要求、MMEL、MRBR、OCAI等11个AEG工作评审项目。同时通过对比分析航空器评审组(简称AEG)评审与型号合格审定之间的区别和联系,为申请人顺利开展AEG工作提出4条建议。%The applicant’ s work of aircraft evaluation is introduced in this paper, and 11 aircraft evaluation items are listed, including operation compliance checklist, type rating and qualification specification, MMEL, MRBR, OCAI, etc. The difference and relation between aircraft evaluation and type certification are contrasted and ana-lyzed at the same time ,and 4 suggestions are provided to the applicant in order to develop aircraft evaluation.

  3. AEg¯$\\overline {\\rm{g}}$IS Experiment: Measuring the acceleration g of the earth’s gravitational field on antihydrogen beam

    Directory of Open Access Journals (Sweden)

    Subieta Vasquez M. A.

    2014-04-01

    Full Text Available The AEg¯ $\\overline {\\rm{g}}$IS experiment [1] aims at directly measuring the gravitational acceleration g on a beam of cold antihydrogen (H¯$\\overline {\\rm{H}}$ to a precision of 1%, performing the first test with antimatter of the (WEP Weak Equivalence Principle. The experimental apparatus is sited at the Antiproton Decelerator (AD at CERN, Geneva, Switzerland. After production by mixing of antiprotons with Rydberg state positronium atoms (Ps, the H¯$\\overline {\\rm{H}}$ atoms will be driven to fly horizontally with a velocity of a few 100 ms−1 for a path length of about 1 meter. The small deflection, few tens of μm, will be measured using two material gratings (of period ∼ 80 μm coupled to a position-sensitive detector working as a moiré deflectometer similarly to what has been done with matter atoms [2]. The shadow pattern produced by the H¯$\\overline {\\rm{H}}$ beam will then be detected by reconstructing the annihilation points with a spatial resolution (∼ 2 μm of each antiatom at the end of the flight path by the sensitive-position detector. During 2012 the experimental apparatus has been commissioned with antiprotons and positrons. Since the AD will not be running during 2013,during the refurbishment of the CERN accelerators, the experiment is currently working with positrons, electrons and protons, in order to prepare the way for the antihydrogen production in late 2014.

  4. AEG评审在型号合格审定中的研究与应用%Research and Application of AEG Evaluation on Type Certification

    Institute of Scientific and Technical Information of China (English)

    段本印

    2015-01-01

    The applicant’ s work of aircraft evaluation is introduced in this paper, and 11 aircraft evaluation items are listed, including operation compliance checklist, type rating and qualification specification, MMEL, MRBR, OCAI, etc. The difference and relation between aircraft evaluation and type certification are contrasted and ana-lyzed at the same time ,and 4 suggestions are provided to the applicant in order to develop aircraft evaluation.%概述了申请人的航空器评审工作,并简要对比分析了运行符合性清单、型别等级和训练要求、MMEL、MRBR、OCAI等11个AEG工作评审项目。同时通过对比分析航空器评审组(简称AEG)评审与型号合格审定之间的区别和联系,为申请人顺利开展AEG工作提出4条建议。

  5. Sequential FDG-PET and induction chemotherapy in locally advanced adenocarcinoma of the Oesophago-gastric junction (AEG: The Heidelberg Imaging program in Cancer of the oesophago-gastric junction during Neoadjuvant treatment: HICON trial

    Directory of Open Access Journals (Sweden)

    Weichert Wilko

    2011-06-01

    Full Text Available Abstract Background 18-Fluorodeoxyglucose-PET (18F-FDG-PET can be used for early response assessment in patients with locally advanced adenocarcinomas of the oesophagogastric junction (AEG undergoing neoadjuvant chemotherapy. It has been recently shown in the MUNICON trials that response-guided treatment algorithms based on early changes of the FDG tumor uptake detected by PET are feasible and that they can be implemented into clinical practice. Only 40%-50% of the patients respond metabolically to therapy. As metabolic non-response is known to be associated with a dismal prognosis, metabolic non-responders are increasingly treated with alternative neoadjuvant chemotherapies or chemoradiation in order to improve their clinical outcome. We plan to investigate whether PET can be used as response assessment during radiochemotherapy given as salvage treatment in early metabolic non-responders to standard chemotherapy. Methods/Design The HICON trial is a prospective, non-randomized, explorative imaging study evaluating the value of PET as a predictor of histopathological response in metabolic non-responders. Patients with resectable AEG type I and II according to Siewerts classification, staged cT3/4 and/or cN+ and cM0 by endoscopic ultrasound, spiral CT or MRI and FDG-PET are eligible. Tumors must be potentially R0 resectable and must have a sufficient FDG-baseline uptake. Only metabolic non-responders, showing a 18FDG-PET scans will be performed before ( = Baseline and after 14 days of standard neoadjuvant therapy as well as after the first cycle of salvage docetaxel/cisplatin chemotherapy (PET 1 and at the end of radiochemotherapy (PET2. Tracer uptake will be assessed semiquantitatively using standardized uptake values (SUV. The percentage difference ΔSUV = 100 (SUVBaseline - SUV PET1/SUVBaseline will be calculated and assessed as an early predictor of histopathological response. In a secondary analysis, the association between the difference

  6. Maskide langemise aeg / Taivo Parbus

    Index Scriptorium Estoniae

    Parbus, Taivo

    2005-01-01

    Res Publica Kuressaare osakonna juhataja sõnul on rahva poliitikast võõrandumise üheks peamiseks põhjuseks pidevad võimutülid Eesti poliitikas. Autor põhjendab vajadust võidelda korruptsiooniga

  7. Aeg niite liimida / Piret Bristol

    Index Scriptorium Estoniae

    Bristol, Piret, 1968-

    2002-01-01

    Arvustus: Hellat, Henn-Kaarel. Niidiliimija. Tallinn : Faatum, 2002. Vastukaja: Hellat, Henn-Kaarel. Selgitus // Sirp (2002) 13. sept., lk. 5. H.-K. Hellat väidab, et ta ei ole müstifikatsiooni "Helilooja Müller" (1997) autor Alex Barber

  8. Animeeritud fantaasiate aeg / Tiit Tuumalu

    Index Scriptorium Estoniae

    Tuumalu, Tiit, 1971-

    2006-01-01

    23. nov. algab PÖFFi raames toimuv animafilmifestifal "Animated Dreams", kus üks esimeste hulgas näidatavaid filme on inglase Phil Mulloy "Perekond Christie". Keskne on šveitsi klassiku Georges Schwizgebeli retrospektiiv, on eesti uut animatsiooni, muusikavideosid jne

  9. Viimane aeg tegutseda / Victoria Punga

    Index Scriptorium Estoniae

    Punga, Victoria, 1977-

    2010-01-01

    Eesti NATO ühingu juhataja ÜRO resolutsioonist nr.1325 - naise rollist rahu, stabiilsuse ja julgeoleku tagamisel. Eesti on üks esimesi, kes on loomas oma konkreetset tegevuskava naiste olukorra parandamiseks maailma relvakonfliktides

  10. Minister Looduse aeg on otsas / Toivo Tootsen

    Index Scriptorium Estoniae

    Tootsen, Toivo, 1943-

    2001-01-01

    Ilmunud ka: Hiiumaa, 31. aug. 2001, lk. 6; Meie Maa, 31. aug. 2001, lk. 2; Kesknädal, 6. sept. 2001, lk. 4; Nädaline, 4. sept. 2001, lk. 5; Sakala, 4. sept. 2001, lk. 2; Nädaline, 4. sept. 2001, lk. 5; Vooremaa, 4. sept. 2001, lk. 2; Virumaa Teataja, 5. sept. 2001, lk. 7. Siseminister peaks tagasi astuma. Autor: Keskerakond. Parlamendisaadik

  11. Nõukogude aeg naaseb piimapakis / Askur Alas

    Index Scriptorium Estoniae

    Alas, Askur, 1973-

    2004-01-01

    Tallinna Piimatööstuse kaubamärgi Turbo-lehm kolmnurkse kartongpakendi kujunduse tegi reklaamifirma DDB Eesti AS, kellega koos valmis ka Turbo-lehma tegelaskuju. Lehma joonistas kunstnik Raivo Tihhonov. Reklaamifirmade kommentaarid

  12. On jõulujuttude aeg / Krista Kumberg

    Index Scriptorium Estoniae

    Kumberg, Krista, 1959-

    2007-01-01

    Arvustus: Minu jõulujutt / [koostajad Jane Liiv, Terje Puistaja, Tiia Toomet]. Tartu : Eesti Rahva Muuseum, 2007 ; Soasepp, Tiia. PPP, ehk, Pesulõksupäkapikkude projekt. Tallinn : Koolibri, 2007 ; Toompere, Harriet. Väikese põhjapõdra uued lood / illustreerinud Anu Kalm. [Tallinn] : Varrak, 2007

  13. Veelgi kehvemate kasumite aeg / James C. Cooper

    Index Scriptorium Estoniae

    Cooper, James C.

    2008-01-01

    Finantssektorist väljaspool tegutsevate ettevõtete kasumid on hämmastavalt stabiilsed püsinud, kuid laenukriis, tõusvad toormaterjali hinnad ja välisriikide majanduste jahtumine ei jäta oma mõju avaldamata ka Ameerikale

  14. Aeg lendab nagu linnutiivul / Udo Knaps

    Index Scriptorium Estoniae

    Knaps, Udo, 1934-

    2009-01-01

    Eestlaste küüditamisest 14. juunil 1941 Põhja-Venemaale ja Siberisse, 25. märtsil 1949 Siberisse. Saarlaste küüditamisest 1. juulil 1949 Siberisse. Sõrulaste küüditamisest Saksa vägede poolt 1944. a. sügisel Saksamaale

  15. USA klaaskatuste murdmise aeg / Kaivo Kopli

    Index Scriptorium Estoniae

    Kopli, Kaivo

    2008-01-01

    USA presidendivalimistel kandideeris esmakordselt afroameeriklane, presidendikandidaatide kampaanias osales naiskandidaat Hillary Clinton. Vt. samas: Obama isa kodukülas palvetati ja valmistuti suureks peoks

  16. Inglite aeg Decos / Tiina Käesel

    Index Scriptorium Estoniae

    Käesel, Tiina, 1943-

    2002-01-01

    Eksponeeritud metallikunstnik Urve Küttneri ehted ja klaasikunstnik Mare Saare ṕte de verrei tehnikas reljeefminiatuuride sari "Inglite elust". Valgustuskunstnik Peeter Mauer, näituse kujundaja Maile Grünberg

  17. Aeg kui heaolu faktor / Urve Kask

    Index Scriptorium Estoniae

    Kask, Urve

    2004-01-01

    Ilmunud ka: Social trends. - Tallinn, 2004, nr. 3, lk. 119-125. Ajakasutusest, kultuuritarbimisest, kulutustest vabale ajale ja kultuurikaupadele, leibkondade sidususest ning sotsiaalsest turvavõrgust. Diagrammid. Tabelid

  18. Täidetud aeg / Urmas Petti

    Index Scriptorium Estoniae

    Petti, Urmas, 1965-

    2013-01-01

    Usust, lootusest ja armastusest lihavõttepühadele eelneval Suurel nädalal; autor kommenteerib ka Kadri Tüüri, Haruki Murakami (romaanist "Kafka mererannas") ja Rein Raua (romaanist "Vend") mõtteid

  19. Antimatter annihilation detection with AEgIS

    CERN Document Server

    Gligorova, Angela

    2015-01-01

    AE ̄ gIS (Antimatter Experiment: Gravity, Interferometry, Spectroscopy) is an antimatter exper- iment based at CERN, whose primary goal is to carry out the first direct measurement of the Earth’s gravitational acceleration on antimatter. A precise measurement of antimatter gravity would be the first precision test of the Weak Equivalence Principle for antimatter. The principle of the experiment is based on the formation of antihydrogen through a charge exchange reaction between laser excited (Rydberg) positronium and ultra-cold antiprotons. The antihydrogen atoms will be accelerated by an inhomogeneous electric field (Stark acceleration) to form a pulsed cold beam. The free fall of the antihydrogen due to Earth’s gravity will be measured using a moiré de- flectometer and a hybrid position detector. This detector is foreseen to consist of an active silicon part, where the annihilation of antihydrogen takes place, followed by an emulsion part coupled to a fiber time-of-flight detector. This overview prese...

  20. "Vaesus on disainerile parim aeg" / Masayo Ave

    Index Scriptorium Estoniae

    Ave, Masayo

    2007-01-01

    Masayo Ave oma elust Euroopas, probleemidest ja kohalike väärtuste arendamisest disainis ja sellest, et puudus paneb aju tööle. Illustratsioonidel valik tema toodetest - öövalgusti Fish Eye, täispuhutav pall-iste, laua- ja seinalamp Genesi, päevapadi Toft jt.

  1. 经左胸腹全胸腔镜食管胃交界区腺癌外科治疗(附17例报告)%The treatment of adenocarcinoma of the esophagogastric junction (AEG) by endoscopic radical oesophagectomy (report of 17 cases)

    Institute of Scientific and Technical Information of China (English)

    宿学家; 张志勇; 朱斌; 谭鑫; 范波

    2014-01-01

    目的:研究探讨胸腔镜下食管胃交界部腺癌(adenocarcinoma of the esophagogastric junction AEG)的手术体位、入路和手术方法的可行性及安全性。方法2011年11月至2014年03月对17例A E G行右侧卧位经左胸腹完全腔镜下根治术:右侧卧位经左胸行腔镜下下段食管的游离及清扫纵隔淋巴结,开膈肌,第七肋间肋弓下、腹直肌外缘做5cm长切口,腔镜辅助下游离贲门、胃及清扫胃左动脉、网膜淋巴结,普通消化道吻合器行食管胃吻合。结果无中转开胸。总手术时间:110-190m i n,平均146m i n。术中无大出血,总出血量30-150m l,平均110m l。共清扫纵隔淋巴结104枚,平均每例6.1枚;清扫腹腔淋巴结133枚,平均每例7.8例。术后住院9-14d,平均11d。住院期间病人无死亡。术后并发症:肺部感染2例,无吻合口漏。结论右侧卧位经左胸腹全腔镜下AEG的外科治疗是可行安全的,近期效果良好,并发症少,达到左侧开胸手术效果。但其长期疗效需要进一步研究、随访。%Objective To study the feasibility and safety of thoracoscopic surgery posturals and methods of the adenocarcinoma of the esophagogastric junction(AEG).Methods A total of 17 patients with AEG were operated with the right lateral endoscopic radical oesophagectomy from November 2011 to March 2014 : freeing the lower esophagus and clean mediastinal lymph nodes using thoracoscopy under the right side of the supine line, then opening the diaphragm and cuting 5cm long incision under the left seventh rib and the outer rectus abdominis, next by endoscopic-assisting freeing cardia, stomach and sweeping left gastric artery and omental lymph nodes, last esophagogastrostomy using a common gastrointestinal stapling. Results No transit thoracotomy. The total operation time :110-190min, average 146min. No massive hemorrhage, bleeding volume 30-150ml, average 110ml. Sweeping a total of 104

  2. ESI-PR10, Vega Alta, Puerto Rico 2000 (Environmental Sensitivity Index Map)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Environmental Sensitivity Index (ESI) maps are an integral component in oil-spill contingency planning and assessment. They serve as a source of information in the...

  3. Seminar "Fragmente eesti tõlkeloost. Nõukogude aeg" / Tõnis Vilu

    Index Scriptorium Estoniae

    Vilu, Tõnis

    2013-01-01

    Tõlkeloo seminarist Eesti Kirjandusmuuseumis 13.12.2012, mille korraldasid Tallinna Ülikooli germaani-romaani keelte ja kultuuride instituut ning Eesti Kirjandusmuuseum. Eestvedajateks olid Anne Lange ja Daniele Monticelli Tallinna Ülikoolist

  4. On aeg taguda trumme Steadfast Jazzi toetuseks / Edward Lucas

    Index Scriptorium Estoniae

    Lucas, Edward, 1962-

    2013-01-01

    2013. a. novembris toimuvast NATO viimase 20 aasta kõige suuremast õppusest, mille käigus harjutavad liitlasväed liikmesriikide territooriumide kaitsmist. Õppus kannab nime SFJZ13 või Steadfast Jazz 2013

  5. Dostojevski aeg / Boris Tuch ; tõlk. Madis Kolk

    Index Scriptorium Estoniae

    Tuch, Boris, 1946-

    2007-01-01

    Dostojevski kangelased Eesti teatrilaval: "Onu unenägu" Vene Teatris (lavastaja Mihhail Tshumatshenko), Matthias Andersoni "Kuritöö, kodutööd, karistus, raha, pensionäri tapmine" Drugoi Teatr (lavastajad Erki Aule ja Artjom Garejev), Eesti - Läti - Leedu vene teatrite koostööprojekt "Russki smehh" (lavastaja Roman Kozak), "Sortsid" Eesti Draamateatris (lavastaja Hendrik Toompere jun.), "Krokodill" Vana Baskini Teatris (lavastaja Mark Rozovski)

  6. Mehel kogu aeg madu peas / Mats Õun

    Index Scriptorium Estoniae

    Õun, Mats, 1964-

    2001-01-01

    Pedagoogi ja psühholoogiharidusega kunstnik ja luuletaja Knut Andresen (sünd. 1938 Friedrichstadtis Põhja-Friisimaal) on tegelnud ka psüühiliselt haigete ja vaimupuudega inimestega, tema maalide näitusest "Mao müüt I" Viljandi Kunstisaalis

  7. Dostojevski aeg / Boris Tuch ; tõlk. Maia Soom

    Index Scriptorium Estoniae

    Tuch, Boris, 1946-

    2007-01-01

    H. Toompere jun. lavastusest "Sortsid" F. Dostojevski romaani järgi, esietendus 14. veebr. Eesti Draamateatris ja M. Tshumatshenko "Onu unenägu" F. Dostojevski jutustuse järgi, esietendus 15. veebr. Vene Teatris

  8. Moodne aeg käib alla / Valner Valme

    Index Scriptorium Estoniae

    Valme, Valner, 1970-

    2008-01-01

    Arvustus: Beigbeder, Frédéric. Armastus kestab kolm aastat : romaan / prantsuse keelest tõlkinud Anti Saar. Tallinn : Varrak, 2008 ; Cusk, Rachel. Arlington Park / tõlkinud Kersti Unt. Tallinn : Varrak, 2007

  9. Aeg teadvustada kliimamuutuse mõju / Sirje Rank

    Index Scriptorium Estoniae

    Rank, Sirje, 1966-

    2007-01-01

    Morgan Stanley analüüsi kohaselt on kliimamuutustel maailmamajandusele shokeeriv mõju. Kaart: Kliimamuutuste võimalikud mõjud Euroopa Liidu rannikualade turismitööstusele. Diagramm: Kliimamuutustest tingitud kahjustuste osakaal riigi või regiooni SKP-st 2,5ʻC soojenemise kohta. Vt. samas: Firmade roheline pale; Kliimaturism tõusul

  10. On aeg, mil vajame enam leiba kui lilli / Ants Juske

    Index Scriptorium Estoniae

    Juske, Ants, 1956-

    2009-01-01

    Marge Monko isikunäitus "Leib ja lilled" Tallinnas Draakoni Galeriis 21. märtsini. Eksponeeritud fotod ja video "Nora õed". Kunstnik on majanduses ja tööhõives asetleidvaid muutusi uurides aluseks võtnud Narva Kreenholmi Manufaktuuri näited

  11. [Aktsioon "Aeg üheks luuletuseks"] / Lauri Eesmaa

    Index Scriptorium Estoniae

    Eesmaa, Lauri

    2003-01-01

    20 aastat Viinis suviti korraldatud luuletekstide eksponeerimine on tänavu laienenud ka teistele Euroopa linnadele. Tänavune teema on "Piirideta luule - kohtumised". PEN-klubi on valinud oma riiki esindama ühe luuletaja

  12. Aeg parandada pearahasüsteemi / Tõnis Lukas

    Index Scriptorium Estoniae

    Lukas, Tõnis, 1962-

    2002-01-01

    Ilmunud ka: Virumaa Teataja 22. august lk. 7, Koit 22. august lk. 6, Põhjarannik, Severnoje Poberezhje, Meie Maa 23. august lk. 2;2, Lääne-Harju Ekspress 24. august lk. 4, Tartu : Postimees 28. august lk. 2, Nädaline 27. august lk. 4, Vooremaa 27.august lk. 8, Valgamaalane 29. august lk. 2, Pärnu Postimees 30. august lk. 10. Endine haridusminister leiab, et väikekoolidele tuleks tagada vähemalt 20% baasfinantseerimine, mis ei sõltu iga õppeaastaga kohe õpilaste arvu kahanemisest. Autor: Isamaaliit. Parlamendisaadik

  13. Aeg on mõtteviisi muuta / Kaie Holm

    Index Scriptorium Estoniae

    Holm, Kaie, 1967-

    2014-01-01

    Kaie Holmi ettekanne raamatukoguhoidjate X kongressil tõi palju vastukaja, autor arendab edasi mõtet, et pensioniealised raamatukoguhoidjad peaksid ise ja vabatahtlikult pensionile jääma, siis oleks noortel, kuhu tööle tulla

  14. Araabia isevalitsejate aeg on ümber saamas

    Index Scriptorium Estoniae

    1999-01-01

    Araabia valitsejatest: Saudi Araabia kuningas Fahd, Liibüa president Muammar Kaddafi, Palestiina president Yassir Arafat, Iraagi president Saddam Hussein, Maroko president Hassan II, Süüria president Hafez Assad, Egiptuse president Hosni Mubarak

  15. Peaminister Juhan Partsil on viimane aeg sisekaemuseks / Ivari Padar

    Index Scriptorium Estoniae

    Padar, Ivari, 1965-

    2004-01-01

    Ilmunud ka: Severnoje Poberezhje, 14. sept. 2004, lk. 2; Pärnu Postimees, 15. sept. 2004, lk. 15; Valgamaalane, 16. sept. 2004, lk. 2; Koit, 16. sept. 2004, lk. 6; Võrumaa Teataja, 16. sept. 2004, lk. 2; Hiiu Leht, 17. sept. 2004, lk. 2; Harjumaa, 17. sept. 2004, lk. 2; Vali Uudised, 17. sept. 2004, lk. 2; Sakala, 17. sept. 2004, lk. 2; Türi Rahvaleht, 17. sept. 2004, lk. 4; Lääne Elu, 18. sept. 2004, lk. 2. Autor arutleb valitsuse ja peaministri tegevuse üle ning leiab, et Juhan Partsile käib peaministri töö üle jõu

  16. Müüride langemise aeg / Reet Varblane

    Index Scriptorium Estoniae

    Varblane, Reet, 1952-

    1999-01-01

    16. jan.-ni 2000 Moderna Museetis avatud Kesk- ja Ida-Euroopa kunsti näitusest "Pärast müüri". Kuraatorid: B. Pejic, D. Elliott, I. Müller-Westermann. Osalejad Eestist: I. Josing, K. Kaljo, L. Siib, J. Toomik. D. Elliotti kataloogi eessõnast (O. Rabini ja C. Andre tööde võrdlus). D. Elliott näituse retseptsioonist Rootsis

  17. Värviline aeg / Liina Hergauk ja Evelin Samuel

    Index Scriptorium Estoniae

    Hergauk, Liina

    2015-01-01

    Värviliste triipudega pontšo, roosa lapitehnikas jakk, kollane puude ja pilvedega kampsun, kirju mantel - autor Jelena Tikunova. Õrn must-punane õlasall - autor Anna Verschik. Sinine põimribadega kampsun - autor Natalia Lill. Helelilla mantel - autor Karin Pärtel. Triibuline kampsun tütarlapsele - autor Liina Hergauk.

  18. EVPde aeg saab otsa / Uku Hänni

    Index Scriptorium Estoniae

    Hänni, Uku, 1943-

    2006-01-01

    Erastamisväärtpaberite kehtivusaeg saab käesoleva aastaga läbi. Sama ka Vooremaa 25. nov. 2006, lk. 4 ; Vooremaa : Palamuse Valla Teataja 25. nov. 2006, lk. 3 ; Kuulutaja 24. nov. 2006, lk. 4 ; Harjumaa 24. nov. 2006, lk. 2 ; Võrumaa Teataja 23. nov. 2006, lk. 2 ; Vali Uudised 29. nov. 2006, lk. 4 ; Vooremaa : Jõgeva Valla Teataja 2. Dets. 2006, lk. 3 ; Koit 2. Dets. 2006, lk. 6 ; Pärnu Postimees 7. Dets. 2006, lk. 15 ; Sakala 28. Dets. 2006, lk. 2

  19. Repsil on aeg hakata haridusega tegelema / Piret Pert

    Index Scriptorium Estoniae

    Pert, Piret

    2006-01-01

    Sotsiaaldemokraatide hinnangul ei valda haridus- ja teadusminister Mailis Reps hariduseelnõude sisu, keeldub arutamast kõiki opositsiooni eelnõusid ega ilmu Riigikogu kultuurikomisjoni eelnõude arutamisele

  20. Paljudele puhkus, noortele ärev aeg / Tõnis Lukas

    Index Scriptorium Estoniae

    Lukas, Tõnis, 1962-

    2002-01-01

    Ilmunud ka: Pärnu Postimees 1. august lk. 10, Lääne-Harju Ekspress 3. august lk. 2, Hiiumaa 1. august lk. 2, Põhjarannik ; Severnoje Poberezhje 1. august lk. 2, Elva Postipoiss 3. august lk. 6, Meie Maa 2. august lk. 2, Hiiu Leht : Käina Kuller 6. august lk. 4, Järva Teataja 6. august lk. 2, Vooremaa 6. august lk. 2, Valgamaalane 6. august lk. 2, Nädaline 8. august lk. 4, Lääne Elu 6. august lk. 4, Tartu : Postimees 9. august lk. 2, Harjumaa 13. august lk. 3, Koit 13. august lk. 6, Harju Uudised 7. august lk. 9. Riigikogu liikme sõnul võivad palgavahed korraliku hariduse ühele osale Eesti elanikest kättesaamatuks teha. Autor: Isamaaliit. Parlamendisaadik

  1. Kiire aeg ja mittepõgenemistsoon / Raivo Kelomees

    Index Scriptorium Estoniae

    Kelomees, Raivo, 1960-

    2000-01-01

    Baseli 20. video, filmi ja uue meedia festivalist "Viper - http://www.viper.ch". Sümpoosionist "Quick Times". Festivali serveris oli Eestist Lauri Tiidemanni video "Käed". Osalemistingimustest. Jaan Tinguely muuseumist (arhitekt Mario Botta). Ilmunud ka kogumikus "Ekraan kui membraan". Tartu, 2007, lk. 81-83

  2. Myanmari aitab üksnes aeg / Mihkel Mutt

    Index Scriptorium Estoniae

    Mutt, Mihkel, 1953-

    2007-01-01

    Myanmaris (Birmas) toimunud buda munkade meeleavaldusest, hunta tegevusest ja suhetest Hiinaga. Autori hinnangul võib valitsev diktatuur aru saada muutuste paratamatusest ja hakata riiki Hiina kombel ülaltpoolt reformima

  3. Hull aeg nõuab segaseid inimesi? / Margit Tõnson

    Index Scriptorium Estoniae

    Tõnson, Margit, 1978-

    2011-01-01

    Režissöör Shinya Tsikamoto film "Kotoko" (Jaapan, 2011), Urszula Antoniaki "Kood: sinine" (Taani-Hollandi 2011), Lisa Aschani "Ahviplikad" (Rootsi 2011), Oliver Hermanuse "Puhas ilu" (Lõuna-Aafrika, 2011) ja Andrei Zvjagintsevi "Jelena" (Venemaa, 2011) Pimedate Ööde Filmifestivalil

  4. Religioosne aeg, religioosne inimene / Ülo Mattheus

    Index Scriptorium Estoniae

    Mattheus, Ülo, 1956-

    1995-01-01

    Vastukaja : Habicht, Juhan. Kõiketeadja on juba meie keskel?// Eesti Ekspress. - 1995. - 25.aug. - Lk.A5; Mattheus, Ülo. Kirjanike kirjavahetus // Eesti Ekspress. - 1995. - 8.sept. - Lk.A5; Liimets, M. [Vastukaja] // Eesti Ekspress. - 1995. - 8.sept. - Lk.A5

  5. Igal õunal oma aeg puu pealt kukkuda / Silvia Viidik

    Index Scriptorium Estoniae

    Viidik, Silvia, 1972-

    2008-01-01

    Apple tegi pühadeperioodi ostuhinnas allahindlusi 10% samal ajal, kui konkurendid tegid kuni 50 protsendilisi soodustusi. Apple'i juhi Steve Jobsi sõnul ta Macide hindu veel kärpima ei hakka, kuna pole väga mures nõudluse vähenemise pärast. Diagrammid: Apple'i aktsia hind; Majandustulemused

  6. "Vana hea aeg" baltisaksa mälestusteraamatus / Ahti Saares

    Index Scriptorium Estoniae

    Saares, Ahti

    2000-01-01

    Baltisakslastest memuariste iseloomustab romantilis-patriootiline suhtumine kodumaasse ja sellest lähtuv kultuuritoojalik, kõrgemat missiooni täitev hoiak. Võrdlus Stefan Zweigi, Sven Danelli ja Kaljo Villako mälestusteraamatutega

  7. Kahuriliha aeg on möödas / Tarmo Leinatamm

    Index Scriptorium Estoniae

    Leinatamm, Tarmo, 1957-2014

    2005-01-01

    Ilmunud ka: Türi Rahvaleht 18. nov., lk. 4, Vali Uudised 18. nov., lk. 2, Sakala 18. nov., lk. 2, Meie Maa 18. nov., lk. 2, Põhjarannik 18. nov., lk. 2, Severnoje Poberezhje 18. nov. lk. 2, Valgamaalane 19. nov., lk. 2, Lääne Elu 19. nov. lk. 2, Hiiu Leht 22. nov. lk. 2, Vooremaa 24. nov. lk. 2. Autor leiab, et kohustuslik ajateenistus Eestis ei taha vajalikku taset

  8. 50 aastat Euroopa integratsiooni: aeg uueks alguseks / Hans-Gert Pöttering

    Index Scriptorium Estoniae

    Pöttering, Hans-Gert, 1945-

    2007-01-01

    Ilmunud ka: Virumaa Teataja, 23. märts 2007, lk. 1; Pärnu Postimees, 24. märts 2007, lk. 19; Koit, 24. märts 2007, lk. 6; Põhjarannik, 24. märts 2007, lk. 2; Severnoje Poberezhje, 24. märts 2007, lk. 2. Euroopa Parlamendi presidendi Hans-Gert Pötteringi mõtted Euroopa Ühenduse asutamislepingu sõlmimise 50. aastapäeva puhul

  9. Aeg on otsustada : Rail Baltica : rahvusvaheline raudtee toob Eestile kasu / Vilja Savisaar-Toomast

    Index Scriptorium Estoniae

    Savisaar-Toomast, Vilja, 1962-

    2011-01-01

    Vilja Savisaar-Toomast on kindel, et Rail Baltica projekt tuleb esitada Euroopa Liidu finantsperioodiks 2014-2020 ning eelistab marsruuti Tallinn-Pärnu-Riia-Kaunas. Tõenäoliselt on võimalik saada projektile Euroopa Liidult ka maksimumtoetus ehk määraga 60-70%

  10. Euroreferendumi aeg jääb veel lahtiseks / Liia Hänni

    Index Scriptorium Estoniae

    Hänni, Liia, 1946-

    2001-01-01

    Euroreferendumi korraldamisest. Autor: Rahvaerakond Mõõdukad. Parlamendisaadik. Ilmunud ka: Hiiumaa, 3. mai 2001, lk. 2; Nädaline, 3. mai 2001, lk. 4; Võrumaa Teataja, 5. mai 2001, lk. 2; Sakala, 8. mai 2001, lk. 2; Virumaa Teataja, 9. mai 2001, lk. 7; Järva Teataja, 11. mai 2001, lk. 2; Lääne Elu, 26. mai 2001, lk. 4

  11. Viimane aeg seksiärile lõpp teha / Kadri Jäätma

    Index Scriptorium Estoniae

    Jäätma, Kadri, 1961-

    2002-01-01

    Põhja- ja Baltimaade ühisseminarist "Naistega kaubitsemine kui kaasaegne orjakaubandus" ja Eesti Avatud Ühiskonna uuringust "Prostitutsioon ja naistekaubandus Tallinna politsei hinnangul". Autor: Isamaaliit. Parlamendisaadik

  12. Aeg ja kosmos. Hilisantiikaja kosmoloogia zoomorfne mälestusmärk

    Directory of Open Access Journals (Sweden)

    Nikolai Sivkov

    2010-12-01

    Full Text Available The protome of the ram from West Bulgaria is a lunisolar calendar of parapegmatic type from the period of antiquity (2nd–4th century AC, which imparts encoded calendrical, cosmological and cosmogonic information.On the protome there are marked synodic, sidereal, and draconic months; lunar, solar and draconic years, as well as different time periods – seasons and cycles. One of the images on the protome’s body can be interpreted as an image of the Draco constellation around the fixed point of the North Pole in the centre of the ecliptic. The symbols of seven luminaries – the Sun, the Moon and five planets – which were known at the beginning of the first millennium are also featured on the protome.In the context of the monument, the presence of the astronomical concept of the world axis and the centre of the ecliptic means that the creators of the ram’s protome perceived it as an omphalos, the sacred centre of the world, the zoomorphic model of the world mountain and the world tree. The suggestion is confirmed by the structural particularities of the protome, by the images of mythological characters and by scenes of cosmogonic motifs. The given artefact represents the conceptualisation of the world in a generalised, syncretised and interdisciplinary way through the language of astronomy, mythology and calendaristics.In the period of antiquity, time and space were perceived as a united integer and as an endless divine beginning connected with the universe and its divine essence. The calendar is an attempt to comprehend the phenomenon of an infinite and cyclic time and use it in practice during the terrestrial life.

  13. Sakala arhitekt Raine Karp : uus aeg nõuab teistsuguseid karpe / Ants Juske

    Index Scriptorium Estoniae

    Juske, Ants, 1956-

    2006-01-01

    Raine Karbi elust ja loomingust, mõtteid Sakala keskuse projekteerimisest, ehitamisest ja ümberehitamisest. Karin Hallas-Murula kommentaar "Tõstis paekivi taas ausse" ja Ivi Eenmaa kommentaar "Teadis raamatukogust hämmastavalt palju"

  14. Ibn Tufail : tema aeg, elu ja koht islami-araabia filosoofia ajaloos / Haljand Udam

    Index Scriptorium Estoniae

    Udam, Haljand, 1936-2005

    2001-01-01

    Varem ilmunud järelsõnana raamatus: Elav Ärganu poeg. Tallinn : Olion, 1992. 12. sajandil Araabia Hispaanias elanud filosoof Abu Bakr Muhammad ibn Abd al-Malik ibn Muhammad ibn Muhammad ibn Tufail. Tema põhiteosest, filosoofilisest jutustusest "Elav Ärganu poeg"

  15. A position sensitive silicon detector for AEgIS (Antimatter Experiment: Gravity, Interferometry, Spectroscopy)

    CERN Multimedia

    Gligorova, A

    2014-01-01

    The AEḡIS experiment (Antimatter Experiment: Gravity, Interferometry, Spectroscopy) is located at the Antiproton Decelerator (AD) at CERN and studies antimatter. The main goal of the AEḡIS experiment is to carry out the first measurement of the gravitational acceleration for antimatter in Earth’s gravitational field to a 1% relative precision. Such a measurement would test the Weak Equivalence Principle (WEP) of Einstein’s General Relativity. The gravitational acceleration for antihydrogen will be determined using a set of gravity measurement gratings (Moiré deflectometer) and a position sensitive detector. The vertical shift due to gravity of the falling antihydrogen atoms will be detected with a silicon strip detector, where the annihilation of antihydrogen will take place. This poster presents part of the development process of this detector.

  16. Plastmassist käekellade aeg hakkab Eestis mööda saama / Kerli Nõu

    Index Scriptorium Estoniae

    Nõu, Kerli

    2007-01-01

    Kellast on saanud staatusesümbol. Kvaliteetkellade müük on kasvamas, kohalikule turule tuleb maailma täpseim mehaaniline kell Šveitsi kaubamärgilt Zenith. Lisad: Kolm suurmängijat; Oksjonid Hongkongis

  17. Offshore-firmade müügis keeruline aeg / Anne Oja

    Index Scriptorium Estoniae

    Oja, Anne, 1970-

    2004-01-01

    Offshore-firmasid ostetakse läbi advokaadibüroode ja audiitorfirmade. 2001. aastal tehtud uuringu järgi käis Eesti ettevõtjate kontrolli all olevatest offshore-ettevõtetest läbi 6 miljardit krooni aastas

  18. EFIKASI LARVASIDA TEMEPHOS TERHADAP AEDES AEgYPTI RESISTEN PADA BERBAGAI KONTAINER

    Directory of Open Access Journals (Sweden)

    Riyani Setiyaningsih

    2015-09-01

    Full Text Available Dengue hemorrhagic fever (DHF is a disease that causes death in Indonesia. Management DHF can be done with contol vector. One technique is the vector control with larvicides temephos. The use temephos already widely used by the people in the control larvae of Ae. aegypti. In the community can be used in applications temephos in some types of containers. The aims of the study to determine the efication of the various containers temephos against Ae. aegypti resistant. Test the efication of the various containers temephos 7 containers are carried on clay, cement, stainless steel, enamel, cans, plastics, plastics recycling and not recycling. Based on the research results temephos still effectively kill mosquito larvae Ae.aegypti resistant containers made   of clay for eight months, enamel effective container for the application of ten months, tin containers, cement and stainless steel effective until the eleventh month. In plastic containers to more effective application temephos fourteenth month=Demam berdarah dengue (DBD merupakan salah satu penyakit yang menyebabkan kematian di Indonesia. Upaya pengendalian DBD dapat dilakukan dengan pengendalian vektor. Salah satu teknik pengendalian vektor adalah dengan larvasida temephos. Penggunaan temephos sudah banyak digunakan masyarakat dalam pengendalian jentik Ae. aegypti. Aplikasi temephos di masyarakat digunakan pada berbagai jenis kontainer. Penelitian bertujuan untuk mengetahui efikasi penggunaan temephos pada berbagai kontainer terhadap Ae. aegypti resisten. Uji efikasi temephos pada berbagai kontainer dilakukan pada 7 kontainer yaitu tanah liat, semen, stainless steel, enamel, kaleng, plastik daur ulang dan plastik tidak daur ulang. Hasil penelitian menunjukkan temephos masih efektif membunuh jentik Ae.aegypti resisten pada kontainer yang terbuat dari tanah liat selama delapan bulan, enamel efektif selama sepuluh bulan, kontainer kaleng, semen dan stainless steel efektif sampai bulan kesebelas. Kontainer plastik aplikasi themephos efektif sampai empatbelas bulan.

  19. EFIKASI LARVASIDA TEMEPHOS TERHADAP AEDES AEgYPTI RESISTEN PADA BERBAGAI KONTAINER

    OpenAIRE

    Riyani Setiyaningsih; Widiarti Widiarti; Lasmiati Lasmiati

    2015-01-01

    Dengue hemorrhagic fever (DHF) is a disease that causes death in Indonesia. Management DHF can be done with contol vector. One technique is the vector control with larvicides temephos. The use temephos already widely used by the people in the control larvae of Ae. aegypti. In the community can be used in applications temephos in some types of containers. The aims of the study to determine the efication of the various containers temephos against Ae. aegypti resistant. Test the efication of the...

  20. Hea töötaja leidmine: viimane aeg on tegutseda / Mariliis Aigro

    Index Scriptorium Estoniae

    Aigro, Mariliis

    2014-01-01

    Maailma parimad ettevõtted-tööandjad panustavad töötajate arendamisse, peavad oluliseks töötajate erialast arengut ja karjääriteed, erilist tähelepanu pööratakse juhtide ja nende järelkasvu arendamisse.

  1. Kai Kaljo: "Aeg-ajalt tuleb ennast peeglist vaadata" / Kai Kaljo ; intervjueerinud Reet Varblane

    Index Scriptorium Estoniae

    Kaljo, Kai, 1959-

    2009-01-01

    Videokunstnik Kai Kaljo oma isikunäitusest "Ainult eestlastele" Tallinna Kunstihoone galeriis, maalikunstisti tähendusest talle, kunstiteostest, mis on teda mõjutanud, Tallinnasse püstitatud Vabadussõja võidusambast

  2. Videoinstallatsiooni aeg pole ümber : muljeid Amsterdami videofestivalilt / Kai Kaljo

    Index Scriptorium Estoniae

    Kaljo, Kai, 1959-

    1999-01-01

    17. World Wide Video Festival 15.-20. IX Amsterdamis Melkwegi keskuses ja Stedelijki muuseumis. Väljapanekust. Kai Kaljolt oli videofestivalil väljas 'One Way to See America'. Seminaride materjalid: wwvf@wwvf.demon ja www.wwvf.nl

  3. 42. Nordische Filmtage Lübeck, vahekokkuvõtete aeg / Lauri Kärk

    Index Scriptorium Estoniae

    Kärk, Lauri, 1954-

    2000-01-01

    Lübeckis toimunud Põhjamaade filmipäevade 42. Nordische Filmtage huvitavamatest filmidest, festivali juhi vahetusest. Baltikumi esindas lühifilmide kassett "Sellised kolm lugu", mille üks autor Ervin Õunapuu ka kohal viibis

  4. Miks meil on kogu aeg kiire? / David Allen ; interv. Endrik Randoja

    Index Scriptorium Estoniae

    Allen, David

    2006-01-01

    Produktiivsuse ekspert vastab küsimustele ettevõetud tegevuste tulemuslikumalt lõpule viimise kohta. Lisa: Aja paremaks kasutamiseks pakub David Allen lisaks veel kolm tõhusat ja lihtsasti rakendatavat põhimõtet

  5. Aeg on hakata parke ja platse arukalt täis ehitama / Tõnu Kann

    Index Scriptorium Estoniae

    Kann, Tõnu, 1957-

    2005-01-01

    Pärnu linnavalitsuse planeerimisosakonna 1996. aasta kesklinna arengu ideekavandist, tühjade kvartalinurkade täisehitamisest, vanade tänavajoonte taastamisest. Kommenteerivad Tõnu Kann ja arhitekt Rein Raie

  6. Henri Tamm : algul küsiti mult kogu aeg autogrammi / Henri Tamm ; interv. Ada Kallam

    Index Scriptorium Estoniae

    Tamm, Henri

    2005-01-01

    Draamaseriaali "Kodu keset linna" lapsnäitleja. Lisaks kolleegid Henrist : M. Veinmann "Tekst on tal esimesena peas", K. Maibaum "Võrdväärne partner suurtele". Artikkel pealkirjaga "Henri Tamm : nüüd saan veidi puhkust" : Linnaleht nr. 22, 3. juuni 2005, A2-A3

  7. Peter Lorange: on aeg hakata uuesti investeerima / Peter Lorange ; intervjueerinud Viljar Rääsk

    Index Scriptorium Estoniae

    Lorange, Peter, 1943-

    2009-01-01

    Šveitsis tegutseva Rahvusvahelise Juhtimiskooli endise juhi hinnangul peaksid valitsused majandusliku languse tingimustes suunama raha infrastruktuuri arendamisse, pakkuma ettevõtjatele maksuvabastusi ja vähendama bürokraatiat

  8. Kehaline kasvatus - mängule kadunud aeg ja ruum? / Joe Noormets

    Index Scriptorium Estoniae

    Noormets, Joe

    2004-01-01

    Autori väitel on üldhariduskooli kehalise kasvatuse tundidesse tunginud üha enam spordile omaseid tegevusmudeleid ja väärtusorientatsioone; mäng ja mängulisus on kehalises kasvatuses kaduma läinud

  9. Avinurmes uus aeg / Sulev Oll ; kommenteerinud Taavi Linnamäe

    Index Scriptorium Estoniae

    Oll, Sulev, 1964-

    2009-01-01

    Teisel jõulupühal pühitsetakse ja hakkavad Avinurmes lööma kolm uut kirikukella, mis valmisid Saksamaal firmas Rudolf Perner GmbH&Co. Kellad paigaldas Mäeväli Orelitöökoda OÜ, ehitus-puutööd tegi Rändmeister OÜ. Vana kirikukell viidi kabelisse. Toetust kellade muretsemiseks saadi PRIA vahendusel

  10. Eesti kaubanduses on võimaluste aeg läbi saanud / Villu Zirnask

    Index Scriptorium Estoniae

    Zirnask, Villu, 1966-

    2007-01-01

    Konsultatsioonifirma A.T. Kearny igaaastane raport Global Retail Development Index hindab olukorda Eesti ja Leedu kaubandusturul igavaks ja leiab, et Balti jaeturg muutub väga küllastunuks. Vt. samas: Majanduskliima: Maailma jaeturgude atraktiivsus; Maailma suurimad jaemüügifirmad; Edetabel: Maailma 10 kõige atraktiivsemat jaekaubandusturgu; Kuidas mõõta turu perspektiivikust?

  11. Gazprom teeb reformi: Ukrainal on aeg gaasipliidid nurka visata / Heiki Suurkask

    Index Scriptorium Estoniae

    Suurkask, Heiki, 1972-

    2009-01-01

    Autor leiab, et Ukraina pole 2006. a. kriisist midagi õppinud, maagaasi tarbimine on veelgi suurenenud. Gaasihinna tõusu korral hakatakse Ukrainas ilmselt üha enam loobuma gaasipliitidest ja -boileritest ning niimoodi kaotaks Gazprom ühe oma suurema turu. Gazprom üritab kõrgemat gaasihinda saavutada ka SRÜ maades. Diagrammid

  12. Aeg ei küsi, kelle hinges laulab lind / Katrin Nielsen

    Index Scriptorium Estoniae

    Nielsen, Katrin

    2000-01-01

    Pärnu Mudaravilas avatud nongratalase Asta Isaku maalinäitusest "Looduses ja lootuses" ning Chaplini kunstikeskuses avatud Tammiste hooldekodu elaniku Aivar Kurvitsa maalinäitusest "Ära reosta loodust"

  13. Erastamisväärtpaberite aeg lõpeb / Signe Kalberg

    Index Scriptorium Estoniae

    Kalberg, Signe, 1959-

    2005-01-01

    Füüsiliste isikute kasutusarvetel on praegu ligi 560 miljonit EVP-krooni. Maa, ehitiste, aktsiate ja muu vallasvara kompenseerimiseks on välja antud üle 8,4 miljardi EVP-krooni, ligi 90% füüsiliste isikute kasutusarveid on praeguseks kas täiesti tühjad või on neile jäänud erastamistehingutes kasutamiseks liiga väike summa. Miljoni kroonini ulatuvaid arveid on füüsilistel isikutel vaid kolm

  14. On aeg mängida! / Ell-Maaja Randküla

    Index Scriptorium Estoniae

    Randküla, Ell-Maaja, 1939-2016

    2005-01-01

    Tartu Mänguasjamuuseumi (Tartu, Lutsu t. 8) sisekujundus. Autorid: Tea Tammelaan, Krista Lepland, Malle Jürgenson, OÜ Laika, Belka & Strelka. Maalitud põrandavaip: Kai Kaljo. Maalitud pitslinad: Tuuli Puhvel. Ehitaja: AS Facio Ehitus. Eesti Sisearhitektide Liidu 2004. a. aastapreemiate konkursil pälvis ajaloolise interjööri preemia. T. Tammelaanest, K. Leplandist ja M. Jürgensonist, OÜ Laika, Belka & Strelka tähtsamad tööd. Ill.: plaan, 23 värv. vaadet

  15. UJI TOKSISITAS BEBERAPA EKSTRAK TANAMAN TERHADAP LARVA AEDES AEgYPTI VEKTOR DEMAM BERDARAH DENGUE

    Directory of Open Access Journals (Sweden)

    Hasan Boesri

    2015-09-01

    Full Text Available Mosquito vector control are still using insecticide  from pyrethroid, carbamates and organophosphates groups because they are effective, having fast result but having less concern for the negative impact to the environment. In order to solve the side effects the side effect, it was necessary to find bio-insecticides which are sustainable, accessible and effective to kill mosquitoes and larvae. In this study the extract of Nicotiana tabacum / tobacco with minimum concentration of 1,56 % was effective to kill larvae with of 100% mortality, with of LC90  0,628 %, LC50 0,194 %. The extract contain chemical dominant alkaloids, Euvodia gravolens / zodiac with minimum concentration 1,56 % the mortality effects 100%, is with LC90 0,628 %, and LC50 0,194 %, the extract concentration 1,56 %, the mortality effect is 100 %, is with LC90a of 0,628% and dominant chemical contains Evodiamine, Alpinia galanga/ of  / galanga with minimum concentration of 1,56 %, the mortality effects is 29,3% and LC90 8,216 %, LC50 2,980 % and dominant chemical contain flavonoids, Andropogon nardus/ lemongrass with minimum concentration of 1,56% the mortality effect is 68% and LC90 4,898 %, LC50 1,068 % and dominant chemical contain of vetivetate acid, rosemary ( Rosmarinus officinalis L with minimum concentration of 1,56% gives mortality effect 78,7% and LC90 3,175 %, LC50 0,659 %. The dominant contain of the chemical is alkaloid = Pengendalian  nyamuk vektor masih menggunakan bahan insektisida dari golongan piretroid, karbamat, dan organofosfat karena  efektif, cepat diketahui hasilnya, namun kurang memperhitungkan dampak negatif terhadap lingkungan. Oleh sebab itu perlu dicari insektisida alernatif, yaitu insektisida nabati yang ramah lingkungan, mudah diperoleh, efektif membunuh nyamuk dewasa dan pradewasa. Pada penelitian diperoleh hasil Nicotiana tabacum/tembakau konsentrasi ekstrak minimal 1,56%, mampu membunuh larva nyamuk sebesar 100 % dan LC90 0,628 %, LC50 0,194 %, (kandungan kimia dominan  alkaloid, Euvodia gravolens/ zodia dengan konsentrasi ekstrak minimal ekstrak 1,56 % mampu memberikan efek kematian 100% dan LC90 0,628.% ,LC50 0,194 % (kandungan kimia dominan  Evodiamine,. Alpinia galanga/lengkuas konsentrasi ekstrak minimal 1,56 % mampu memberikan efek kematian 29,3 % dan LC90 8,216 % ,LC50 2,980 % (kandungan kimia dominan  flavonoida, Andropogon nardus/serai wangi konsentrasi ekstrak minimal 1,56% mampu membunuh larva nyamuk sebesar 68 % dan LC90 4,898 %, LC50 1,068 % ( kandungan kimia dominan  asam vetivetate, Rosemary (Rosmarinus officinalis L konsentrasi ekstrak minimal 1,56%, memberikan efek kematian sebanyak 78,7 % dan LC90 0,659 %, LC50 3,175 % (kandungan kimia dominan alkaloid.

  16. Primary structures of two ribonucleases from ginseng calluses - New members of the PR-10 family of intracellular pathogenesis-related plant proteins

    NARCIS (Netherlands)

    Moiseyev, GP; Fedoreyeva, LI; Zhuravlev, YN; Yasnetskaya, E; Jekel, PA; Beintema, JJ

    1997-01-01

    The amino acid sequences of two ribonucleases from a callus cell culture of Panax ginseng were determined, The two sequences differ at 26% of the amino acid positions, Homology was found with a large family of intracellular pathogenesis-related proteins, food allergens and tree pollen allergens from

  17. Elutervik ja aeg : murdkem püramiidi võim / Sigmund Kvaloy ; tõlk. Tiiu Speek

    Index Scriptorium Estoniae

    Kvaloy, Sigmund

    2005-01-01

    Nüüdisaegsest ökofilosoofiast, tööstusliku kasvu ühiskonna ja eluliste vajaduste ühiskonna vastuoludest ning Norra ökopoliitikast. Lisatud lühiülevaade autori elukäigust. Vt. ka: David Rothenberg. Elutervik ja elulised vajadused: Eesti tee. Lk. 549

  18. Lineaarse kunstiajaloo aeg on otsas / Edit András ; intervjueerinud R[eet] V[arblane

    Index Scriptorium Estoniae

    András, Edit

    2010-01-01

    Vestlus ungari kunstiteoreetiku- ja ajaloolase Edit Andrásiga, kes osales Tallinnas USA Clarki instituudi ja EKA Kunstiteaduste Instituudi seminaril, kus arutati Kesk- ja Ida-Euroopa kunstiajaloo kirjutamisviiside üle. Seminari tekkimisest, kokkusaamise põhjustest, seminari järeldustest, Eesti ja Ungari kunstiajaloo kirjutamise erinevatest põhimõtetest jm.

  19. President Arnold Rüütel : ekstensiivse arengu aeg on möödas / Arnold Rüütel

    Index Scriptorium Estoniae

    Rüütel, Arnold, 1928-

    2001-01-01

    Eesti-suguses väikeriigis saab ekstensiivset arengumudelit kasutada vaid piiratud aja jooksul ning vajalik on üleminek intensiivsele ja samaaegselt ka ühiskonnakesksele arengumudelile. Kommenteerib A. Luukas

  20. Väljastpoolt Euroopat Eestisse lendajate check-inþi aeg pikeneb / Jan Jõgis-Laats

    Index Scriptorium Estoniae

    Jõgis-Laats, Jan

    2007-01-01

    1. juulist 2007 jõustunud riigipiiri seaduse muudatusest, mille kohaselt on lennufirmadel kohustus edastada piirivalvele Euroopa Liidu välistest riikidest Eestisse saabuvate lennureisijate isikuandmed. Lisatud: seadusemuudatuse nõuab lisaandmeid

  1. Deleuze, filmikunst ja rahvuslik identiteet : narratiivi aeg rahvuslikes kontekstides / David Martin-Jones ; tõlkinud Eva Näripea

    Index Scriptorium Estoniae

    Martin-Jones, David

    2011-01-01

    Antakse ülevaade Gilles Deleuze'i filmitekstidest "Kino 1 : liikumispilt" (Cinếma-1 : l'image-mouvement) ja "Kino 2 : ajapilt" (Cinếma-2 : l'image-temps) ning K. Bhabha artiklist "DissemiNatsioon" kogumikust "Rahvus ja jutustamine" (Nation and Narration,1990), kus käsitletakse ideesid narratiivist, ajast ja rahvuslikust identiteedist. Uuritakse, kuidas käsitletakse narratiivi aja kaudu ajalugu ja identiteeti ning missugust toimet avaldab see rahvusliku identiteedi konstrueerimisele

  2. Rattarändurid : naistel on mõistlik mehed aeg-ajalt kodust ära lasta / Greta Kaupmees

    Index Scriptorium Estoniae

    Kaupmees, Greta

    2007-01-01

    Täna hommikul stardib Terevisiooni ärataja Priit Kuusk koos viie ränduriga Tallinnast Katmandusse, et sealt edasi asuda maid avastama jalgratastel. Seiklejatel on plaanis sõita nelja kuuga ligi 4000 kilomeetrit ehk Katmandust Teherani. Rattaretkest valmib ETV-s taas dokumentaalsari "Ehh, uhhuduur"

  3. Elutervik ja aeg : murdkem püramiidi võim / Sigmund Kvaloy ; tõlk. Tiiu Speek

    Index Scriptorium Estoniae

    Kvaloy, Sigmund

    2005-01-01

    Nüüdisaegsest ökofilosoofiast, tööstusliku kasvu ühiskonna ja eluliste vajaduste ühiskonna vastuoludest ning Norra ökopoliitikast. Lisatud lühiülevaade autori elukäigust. Vt. ka: David Rothenberg. Elutervik ja elulised vajadused: Eesti tee

  4. Professor Trechsel : Eesti e-valimiste aeg on liiga lühike / Alexander H. Trechsel ; interv. Askur Alas

    Index Scriptorium Estoniae

    Trechsel, Alexander H.

    2006-01-01

    e-valimistele pühendatud rahvusvahelisel konverentsil esinenud Genfi ülikooli e-demokraatia keskuse juhataja, poliitikateaduste professor Eesti e-valimistest ja e-valimiste tulevikust. Vt. samas: Askur Alas. Konverentsil analüüsiti e-valimiste kogemusi; Eestis hääletas digitaalselt 9317 valijat

  5. Ida-Virul on aeg oma võimalused välja mängida / Mart Laar

    Index Scriptorium Estoniae

    Laar, Mart, 1960-

    2008-01-01

    Ilmunud ka: Severnoje Poberezhje : Ida-Virumaa Zavtra 15. mai, lk. 4. Ekspeaministrist IRL-i esimehe Mart Laari hinnangul on Ida-Virumaa eduka arengu võtmeküsimused korruptsiooni ja kuritegevuse vähendamises ning ettevõtluskeskkonna parandamises

  6. Tallinna Raekoda. Aeg ja lugu = Tallinn Town Hall - Its Story Through Time / Elvira Liiver Holmström

    Index Scriptorium Estoniae

    Liiver Holmström, Elvira

    2004-01-01

    Raekoja kujunemislugu. Esindussaalid, ameti- ja tarberuumid. Raekoda 17.-20. sajandil. Hoone ümberehitamist 1402-1404 juhtis kiviraidur Ghercke. Hinrik Hartmanni poolt valatud kell paigutati torni 1586. a. 17. sajandil tegi hilisrenessanss-stiilis torni joonised Geiger Graff, lääneviilule uue harjakivi ja tuulelippu hoidva lõvi Hans Damm, lohepeakujulised veesülitid ja tuulelipu lipu Daniel Pöppel, raesaali paigutati Johann Akeni lünettmaalid, nende all paikneva puitnikerdfriisi valmistasid Elert Thiele ja Joachim Armbrust. 1859. a. ümberehitusprojekti koostas Christoph August Gabler

  7. Mis on jumal? / Olev Remsu

    Index Scriptorium Estoniae

    Remsu, Olev, 1947-

    1995-01-01

    Vastukaja : Raudsepp, Jüri. Kas Eesti Aeg on mööda saamas?// Eesti Aeg. - 1995. - 19.juuli. - Lk.11; Remsu, Olev. Lugupeetud Jüri Raudsepp.!// Eesti Aeg. - 1995. - 19.juuli. - Lk.11. Uring, Priit. Remsule sekundeerides // Eesti Aeg. - 1995. - 2.aug. - Lk.11

  8. Thermodynamic characterization of the PR-10 allergens Bet v 1, Api g 1 and Dau c 1 and pH-dependence of nApi g 1 and nDau c 1

    NARCIS (Netherlands)

    Bollen, M.A.; Wichers, H.J.; Helsper, J.P.F.G.; Savelkoul, H.F.J.; Boekel, van M.A.J.S.

    2010-01-01

    Natural and recombinant Bet v 1, the major birch pollen allergen, and homologous allergens, Api g 1 and Dau c 1, from celery and carrot, respectively, were studied by CD spectroscopy under conditions of varying denaturant concentration, pH and temperature to determine fundamental thermodynamic param

  9. A SEMIOLOGIC APPROACH TO AUDIT EXPECTATIONS GAP

    OpenAIRE

    Ciolpan Daniela; Dobroteanu Camelia Liliana; Dobroteanu Laurentiu

    2009-01-01

    Audit expectations gap (AEG) is one of the most debated phenomena animating the international scientific research scene. The volume of papers focused on defining the AEG concept, examining its determinants, implications, and mechanisms to minimize the gap

  10. Ilmus väärt artiklikogumik / Arvo Tering

    Index Scriptorium Estoniae

    Tering, Arvo, 1949-

    2008-01-01

    Arvustus: Ajalookirjutaja aeg = Aetas historicum / Eesti Rahvusraamatukogu ; koost. Piret Lotman. - Tallinn, 2008. - 247 lk. - (Eesti Rahvusraamatukogu toimetised. A, Raamat ja aeg, 1736-6984 ; nr. 1) ; (Eesti Rahvusraamatukogu toimetised ; 11)

  11. Kestmine kui probleem / Marju Lauristin

    Index Scriptorium Estoniae

    Lauristin, Marju, 1940-

    1987-01-01

    Vestlus vabariikliku teatrifestivali "Tartu ' 87" žürii liikmega : A. Kitzbergi "Libahunt" Rakvere Teatris, A. H. Tammsaare-A.-E. Kerge "Aeg tulla, aeg minna" Vanemuises, T. Ajtmatovi "Ja sajandist on pikem päev" Ugalas

  12. Poliitika ja poeesia eesti graafikas : Tallinna XIV graafikatriennaal ja "Impact 5" / Andri Ksenofontov

    Index Scriptorium Estoniae

    Ksenofontov, Andri, 1962-

    2007-01-01

    Tallinna XIV graafikatriennaalist "Poliitiline/Poeetiline" ning rahvusvahelisest graafikakonverentsist "Impact 5" teemal "Lõigatud aeg" ja selle raames avatud näitustest (Vappu Thurlowi kureeritud "Lõigatud aeg" Adamson-Ericu muuseumis jpt.)

  13. A SEMIOLOGIC APPROACH TO AUDIT EXPECTATIONS GAP

    Directory of Open Access Journals (Sweden)

    Ciolpan Daniela

    2009-05-01

    Full Text Available Audit expectations gap (AEG is one of the most debated phenomena animating the international scientific research scene. The volume of papers focused on defining the AEG concept, examining its determinants, implications, and mechanisms to minimize the gap

  14. Muutuste ja jätkuvuse aeg : Euroopa Liidu teemalise diskussiooni võrdlev analüüs ajalehtedes Postimees ja Helsingin Sanomat / Sigrid Kaasik-Krogerus

    Index Scriptorium Estoniae

    Kaasik-Krogerus, Sigrid

    2007-01-01

    "Tagasi Euroopasse" on olnud viimastel kümnenditel üks olulisemaid märksõnu nii Eesti kui Soome arengus. Euroopa Liidu teema Eesti ja Soome avalikus diskussioonis võimaldab analüüsida ka kahe riigi rahvusliku identiteedi lugu, kus oluline roll on ajakirjandusel

  15. Aeg on Soome ja Rootsi töösturid kinni püüda / Taivo Paju, Kärt Blumberg

    Index Scriptorium Estoniae

    Paju, Taivo, 1968-

    2008-01-01

    Ettevõtete edutegurite üle arutlevad Arengufondi juhatuse liige Heido Vitsur, Balti Uuringute Instituudi juhatuse esimees Marek Tiits, TTÜ mehhaanikateaduskonna professor Rein Küttner. Vt. samas: Ketlin Priilinn. Metsikult kallis küll, kuid seda väärt; Merike Teder. Uute seadmeteta oleksime kadunud (ilmunud ka Molodjozh Estonii : Biznes Sreda 16. apr. lk. 8); Taivo Paju. Pelmeenikuningas Ivan Zakotei (ilmunud ka Molodjozh Estonii : Biznes Sreda 30. apr. lk. 11); Poolteist kuud ja tehas töökorras

  16. Apellatsioonimenetluse aeg tsiviilasjades Eesti Vabariigis ja seda mõjutavad tegurid : [magistritöö] / Sirje Viherpuu ; Tartu Ülikool, õigusteaduskond ; juhendaja: Mare Merimaa

    Index Scriptorium Estoniae

    Viherpuu, Sirje, 1969-

    2013-01-01

    Mõistlikust menetlusajast ja selle eesmärgist tsiviilkohtumenetluses, menetlusajast Eesti Vabariigis apellatsioonimenetluses tsiviilasjade läbivaatamisel, takistavatest teguritest ja võimalustest menetluse kiirendamiseks

  17. 1 küsimus noorele juhile : Kui raha ja aeg ei piira, siis mida tahaksite elus juurde õppida? / Jekaterina Eilart, Andres Urb, Mariana Kolesnik... [jt.

    Index Scriptorium Estoniae

    2010-01-01

    Küsimusele vastavad Hamburg ja Partnerid töötaja Jekaterina Eilart, Sampo Life Eesti varahaldur Andres Urb, Marati peadisainer Mariana Kolesnik, katusefirma Evari juht Rain Kala, endine tudengiorganisatsiooni AIESEC Eesti president Lauri Lahi ning internetiturundaja Marjam Vaher

  18. Knockdown of astrocyte elevated gene-1 inhibits tumor growth and modifies microRNAs expression profiles in human colorectal cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Sujun [East Department of Gastroenterology, Institute of Geriatrics, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080 (China); Southern Medical University, Guangzhou, Guangdong 510515 (China); Wu, Binwen, E-mail: wubinwengd@aliyun.com [East Department of Gastroenterology, Institute of Geriatrics, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080 (China); Li, Dongfeng; Zhou, Weihong; Deng, Gang; Zhang, Kaijun; Li, Youjia [East Department of Gastroenterology, Institute of Geriatrics, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080 (China)

    2014-02-14

    Highlights: • AEG-1 expression in CRC cell lines and down-regulation or upregulation of AEG-1 in vitro. • Knockdown of AEG-1 inhibits cell proliferation, colony formation and invasion. • Upregulation of AEG-1 enhances proliferation, invasion and colony formation. • Knockdown of AEG-1 accumulates G0/G1-phase cells and promotes apoptosis in CRC cells. • AEG-1 knockdown increases 5-FU cytotoxicity. - Abstract: Astrocyte elevated gene-1 (AEG-1), upregulated in various types of malignancies including colorectal cancer (CRC), has been reported to be associated with the carcinogenesis. MicroRNAs (miRNAs) are widely involved in the initiation and progression of cancer. However, the functional significance of AEG-1 and the relationship between AEG-1 and microRNAs in human CRC remains unclear. The aim of this study was to investigate whether AEG-1 could serve as a potential therapeutic target of human CRC and its possible mechanism. We adopted a strategy of ectopic overexpression or RNA interference to upregulate or downregulate expression of AEG-1 in CRC models. Their phenotypic changes were analyzed by Western blot, MTT and transwell matrix penetration assays. MicroRNAs expression profiles were performed using microarray analysis followed by validation using qRT-PCR. Knockdown of AEG-1 could significantly inhibit colon cancer cell proliferation, colony formation, invasion and promotes apoptosis. Conversely, upregulation of AEG-1 could significantly enhance cell proliferation, invasion and reduced apoptisis. AEG-1 directly contributes to resistance to chemotherapeutic drug. Targeted downregulation of AEG-1 might improve the expression of miR-181a-2{sup ∗}, -193b and -193a, and inversely inhibit miR-31 and -9{sup ∗}. Targeted inhibition of AEG-1 can lead to modification of key elemental characteristics, such as miRNAs, which may become a potential effective therapeutic strategy for CRC.

  19. Knockdown of astrocyte elevated gene-1 inhibits tumor growth and modifies microRNAs expression profiles in human colorectal cancer cells

    International Nuclear Information System (INIS)

    Highlights: • AEG-1 expression in CRC cell lines and down-regulation or upregulation of AEG-1 in vitro. • Knockdown of AEG-1 inhibits cell proliferation, colony formation and invasion. • Upregulation of AEG-1 enhances proliferation, invasion and colony formation. • Knockdown of AEG-1 accumulates G0/G1-phase cells and promotes apoptosis in CRC cells. • AEG-1 knockdown increases 5-FU cytotoxicity. - Abstract: Astrocyte elevated gene-1 (AEG-1), upregulated in various types of malignancies including colorectal cancer (CRC), has been reported to be associated with the carcinogenesis. MicroRNAs (miRNAs) are widely involved in the initiation and progression of cancer. However, the functional significance of AEG-1 and the relationship between AEG-1 and microRNAs in human CRC remains unclear. The aim of this study was to investigate whether AEG-1 could serve as a potential therapeutic target of human CRC and its possible mechanism. We adopted a strategy of ectopic overexpression or RNA interference to upregulate or downregulate expression of AEG-1 in CRC models. Their phenotypic changes were analyzed by Western blot, MTT and transwell matrix penetration assays. MicroRNAs expression profiles were performed using microarray analysis followed by validation using qRT-PCR. Knockdown of AEG-1 could significantly inhibit colon cancer cell proliferation, colony formation, invasion and promotes apoptosis. Conversely, upregulation of AEG-1 could significantly enhance cell proliferation, invasion and reduced apoptisis. AEG-1 directly contributes to resistance to chemotherapeutic drug. Targeted downregulation of AEG-1 might improve the expression of miR-181a-2∗, -193b and -193a, and inversely inhibit miR-31 and -9∗. Targeted inhibition of AEG-1 can lead to modification of key elemental characteristics, such as miRNAs, which may become a potential effective therapeutic strategy for CRC

  20. 75 FR 62519 - Rocky Mountain Natural Gas LLC; KeySpan Gas East Corporation; ECOP Gas Company, LLC; MGTC, Inc...

    Science.gov (United States)

    2010-10-12

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. PR10-130-000; PR10-131-000; PR10-132-000; PR10-133-000; PR10-134-000; PR10-135-000; PR10-136-000 (Not Consolidated)] Rocky Mountain Natural Gas LLC; KeySpan Gas East Corporation; ECOP Gas Company, LLC;...

  1. Lentivirus-Mediated Knockdown of Astrocyte Elevated Gene-1 Inhibits Growth and Induces Apoptosis through MAPK Pathways in Human Retinoblastoma Cells.

    Directory of Open Access Journals (Sweden)

    Ying Chang

    Full Text Available To explore expression and function of astrocyte elevated gene-1 (AEG-1 in human retinoblastoma (RB.The expression of AEG-1 in histological sections of human RBs and in RB cell lines was examined using immunohistochemical staining and RT-PCR and Western blotting respectively. We knocked down AEG-1 gene levels by AEG-1-siRNA lentivirus transfection of human RB cell lines SO-RB50 and Y79, and using an MTT assay, we assessed the role of AEG-1 on RB cell proliferation. The biological significance of lentivirus transfection induced AEG-1 down-regulation was examined by assessing the apoptosis rate in the transfected RB cells by Annexin V-APC staining and flow cytometry. We additionally measured the expression of Bcl-2, Bax, cleaved-caspase-3 and caspase-3, and the phosphorylation and non-phosphorylation alternation of MAPKs.AEG-1 expression was detected to be strongly positive in the histological slides of 35 out of 54 (65% patients with RB. AEG-1 expression increased significantly (P<0.05 with tumor stage. In the RB cell lines SO-RB50, Y79 and WERI-RB1 as compared with retinal pigment epithelium cells, expression of AEG-1 mRNA and AEG-1 protein was significantly higher. In AEG-1-siRNA lentivirus transfected cell cultures as compared with negative control lentivirus transfected cell cultures, levels of AEG-1 mRNA and of AEG-1 protein (P<0.05 and cell growth rates (P<0.01 were significantly lower, and apoptosis rate (P<0.001, Bax/Bcl-2 ratio and cleaved-caspase-3 protein level were significantly increased. The P-ERK/ERK ratio was significantly decreased in the AEG-1-siRNA lentivirus transfected cell lines.Expression of AEG-1 was associated with RB, in histological slides of patients and in cell culture experiments. Lentivirus transfection induced knockdown of AEG-1 had a tumor suppressive effect, potentially by tumor cell apoptosis induction through inhibition of ERK.

  2. Ikka teel / Marian Kivila

    Index Scriptorium Estoniae

    Kivila, Marian

    2009-01-01

    Rahvusvahelise tegevuskunstifestivali "Diverse Universe 2009" üritustest Tallinna Kultuurikatlas, Tartu pärmitehases ja galeriis Ferrodrum ning Paides, kus toimus kunstifestval "Aeg. Ruum. Liikumine"

  3. Appi räsitud firmadele. Pohjola: praegu on tulekuks õige aeg / Reima Rytsölä, Arja Jurmu, Jorma Alanne ; intervjueerinud Kadrin Karner ; kommenteerinud Andreas Laane, Hans Pajoma, Robert Kitt

    Index Scriptorium Estoniae

    Rytsölä, Reima

    2011-01-01

    Pohjola pank avas Tallinnas kontori äriklientide teenindamiseks. Küsimustele vastavad Pohjola Groupi pangandusoperatsioonide juht Reima Rytsölä, Pohjola panga Eesti filiaali juht Arja Jurmu ja Pohjola Balti panganduse juht Jorma Alanne

  4. Astrocyte elevated gene-1 regulates CCL3/CCR5-induced epithelial-to-mesenchymal transition via Erk1/2 and Akt signaling in cardiac myxoma.

    Science.gov (United States)

    Shi, Ping; Fang, Changcun; Pang, Xinyan

    2015-09-01

    In recent years, astrocyte elevated gene-1 (AEG-1) has been reported as a key mediator that is involved in the epithelial-to-mesenchymal transition (EMT) process. However, the mechanisms underlying CCL3/CCR5-AEG-1 pathway-mediated EMT in cardiac myxoma (CM) has not been well featured till now. We used immnohistochemistry and immunoblotting to assess the expression of CCR5 and AEG-1 in 30 cases of CM tissues and cells. Subsequently, cultured CM cells were treated with si-AEG-1 or si-CCR5 and then subjected to in vitro assays. We observed that CCR5 and AEG-1 proteins were highly expressed in CM tissues (73.3 and 76.7%, respectively) and closely correlated with tumor size (>5 cm). Importantly, we validated the expression of AEG-1, p-Erk1/2, p-Akt, vimentin, N-cadherin and MMP2 increased in the CM cell with CCL3 treatment in a time- and concentration-dependent manner. When CM cells were treated with si-CCR5, the expression of AEG-1, p-Erk1/2, p-Akt, vimentin, N-cadherin and MMP2 was downregulated. In addition, when CM cells were treated with si-AEG-1, the expression of p-Erk1/2, p-Akt, vimentin, N-cadherin and MMP2 was also downregulated. Using the cell cycle and proliferation assay, the knockdown of AEG-1 inhibited the entry of G1 into S phase and the proliferation capacity of CM cells. In conclusion, AEG-1 mediates CCL3/CCR5-induced EMT development via both Erk1/2 and Akt signaling pathway in CM patients, which indicates CCL3/CCR5-AEG-1-EMT pathway could be suggested as a useful target to affect the progression of CM.

  5. Knockdown of astrocyte elevated gene-1 inhibits proliferation and enhancing chemo-sensitivity to cisplatin or doxorubicin in neuroblastoma cells

    Directory of Open Access Journals (Sweden)

    Xie Li

    2009-02-01

    Full Text Available Abstract Background Astrocyte elevated gene-1 (AEG-1 was originally characterized as a HIV-1-inducible gene in primary human fetal astrocyte. Recent studies highlight a potential role of AEG-1 in promoting tumor progression and metastasis. The aim of this study was to investigate if AEG-1 serves as a potential therapeutic target of human neuroblastoma. Methods We employed RNA interference to reduce AEG-1 expression in human neuroblastoma cell lines and analyzed their phenotypic changes. Results We found that the knockdown of AEG-1 expression in human neuroblastoma cells significantly inhibited cell proliferation and apoptosis. The specific downregulation induced cell arrest in the G0/G1 phase of cell cycle. In the present study, we also observed a significant enhancement of chemo-sensitivity to cisplatin and doxorubicin by knockdown of AEG-1. Conclusion Our study suggests that overexpressed AEG-1 enhance the tumorogenic properties of neuroblastoma cells. The inhibition of AEG-1 expression could be a new adjuvant therapy for neuroblastoma.

  6. Inhibition of Hypoxia Inducible Factor Alpha and Astrocyte-Elevated Gene-1 Mediates Cryptotanshinone Exerted Antitumor Activity in Hypoxic PC-3 Cells

    Directory of Open Access Journals (Sweden)

    Hyo-Jeong Lee

    2012-01-01

    Full Text Available Although cryptotanshinone (CT was known to exert antitumor activity in several cancers, its molecular mechanism under hypoxia still remains unclear. Here, the roles of AEG-1 and HIF-1α in CT-induced antitumor activity were investigated in hypoxic PC-3 cells. CT exerted cytotoxicity against prostate cancer cells and suppressed HIF-1α accumulation and AEG-1 expression in hypoxic PC-3 cells. Also, AEG-1 was overexpressed in prostate cancer cells. Interestingly, HIF-1α siRNA transfection enhanced the cleavages of caspase-9,3, and PAPR and decreased expression of Bcl-2 and AEG1 induced by CT in hypoxic PC-3 cells. Of note, DMOG enhanced the stability of AEG-1 and HIF-1α during hypoxia. Additionally, CT significantly reduced cellular level of VEGF in PC-3 cells and disturbed tube formation of HUVECs. Consistently, ChIP assay revealed that CT inhibited the binding of HIF-1α to VEGF promoter. Furthermore, CT at 10 mg/kg suppressed the growth of PC-3 cells in BALB/c athymic nude mice by 46.4% compared to untreated control. Consistently, immunohistochemistry revealed decreased expression of Ki-67, CD34, VEGF, carbonic anhydrase IX, and AEG-1 indices in CT-treated group compared to untreated control. Overall, our findings suggest that CT exerts antitumor activity via inhibition of HIF-1α, AEG1, and VEGF as a potent chemotherapeutic agent.

  7. Saksa vastupanu ja prantsuse tundeelu / Endel Link

    Index Scriptorium Estoniae

    Link, Endel

    2005-01-01

    Berliini 55. filmifestivalist ja festivali filmidest : sakslase Marc Rothemundi "Sophie Scholl - viimased päevad", prantslase Régis Wargineri "Man to Man" ja teise prantslase André Téchiné "Muutunud aeg"

  8. USA maksumaksja nuriseb HIV-ennetusraha kulutamise üle / Anti Liiv

    Index Scriptorium Estoniae

    Liiv, Anti, 1946-

    2004-01-01

    Henry J. Kaiseri perekonna sihtasutus uuris tänavu, kuidas on USA maksumaksjad rahul nende raha kinkimisega välisriikidele HIV-vastaseks võitluseks. Autori arvates oleks ka Eestis aeg selliseid uuringuid korraldada

  9. Tegevuskunstnikud üllatavad balliga / Eero Ijavoinen ; interv. Arnika Tegelmann

    Index Scriptorium Estoniae

    Ijavoinen, Eero, 1963-

    2000-01-01

    Intervjuu tegevuskunstifestivali "Aeg Ruum Liikumine" korraldaja Eero Ijavoineniga; festivalil osalevad Leonhard Lapin, Ilmar Kruusamäe, Kaarina Ormio, Roi Vaara, David Rotenberg, Jaanika Peerna, Gert Hatsukov, vene rühmitus The Blind

  10. Õigusterminid ja õigusharidus, erialase oskussõnavara omandamine / Merike Ristikivi

    Index Scriptorium Estoniae

    Ristikivi, Merike, 1973-

    2012-01-01

    Juristide jaoks olulise erialase oskussõnavara omandamisest kolmel perioodil: 1918-1940, nõukogude aeg ning taasiseseisvusaeg. Õigusterminoloogia omandamisest ning õppevahenditest, mille alusel ülikoolis juriidilisi termineid õpetati ja õpiti

  11. Maapoisi tee linnakirjandusse / Mati Unt

    Index Scriptorium Estoniae

    Unt, Mati, 1944-2005

    1999-01-01

    Mälestusi keskkooliajast Tartus (1958-1962). Ettekanne Tartu Kirjanduse Majas 1999. a. mais toimunud Soome-Eesti ühisseminaril. Ilmunud ka kogumikus: Taasleitud aeg = Kadonneen ajan arvoitus. Tartu, 2000, lk. 24-32

  12. Estonia plans to build nuclear power plant / Jana Belugina

    Index Scriptorium Estoniae

    Belugina, Jana

    2009-01-01

    Eesti Energia on tuumaelektrijaama ehitamiseks välja valinud kuus asukohta. Seadusandlikke otsuseid tuumaelektrijaama ehitamise kohta saab vastu võtta alates 2014. aastast ning tuumareaktori valmimise aeg on 2025. aasta

  13. Birgit Õigemeele jõulualbumi eelesitlus

    Index Scriptorium Estoniae

    2008-01-01

    Rõivabränd Pepe Jeans London korraldab Tallinnas Kristiine keskuses Birgit Õigemeele minikontserdi ja jõulualbumi "Ilus Aeg" plaadiesitluse (heliplaadil teevad kaasa ka Uku Suviste ja ETV lastekoor Lii Leitmaa juhatusel)

  14. Geneetiliselt muundatud? / Epp Petrone

    Index Scriptorium Estoniae

    Petrone, Epp, 1974-

    2006-01-01

    Ameerikas on geneetiliselt muundatud toitu kasvatatud 20 ja ulatuslikult toodetud kaheksa aastat, ning juba mõni aeg tagasi kadus kontroll selle üle, mis supermarketites müüdavast tavatoidust on GMO-vaba ja mis mitte

  15. New Era stands against Muiznieks / Aaron Eglitis

    Index Scriptorium Estoniae

    Eglitis, Aaron

    2005-01-01

    Läti juhtiv parempartei Uus Aeg ning rahvuslikud Isamaa Eest ja Vabadus ei toeta koalitsiooni kuuluva Läti Esimese Partei liikme Nils Muiznieksi kandidatuuri inimõiguste osakonna juhiks kuna viimane olevat ebakompetentne ja eelarvamustega

  16. Ajamere lainetes / Jana Savi

    Index Scriptorium Estoniae

    Savi, Jana

    2012-01-01

    Psühholoogilisest aja käsitlusest - minevik, olevik, tulevik ning teraapiatest (elu)probleemide lahendamiseks. Hetkes elamisest ning neljandast mõõtmest (aeg ja ruum), aegruumi mustritest ja maiade kalendrist

  17. Eesti Energia, vara veel! / Väino Sarnet

    Index Scriptorium Estoniae

    Sarnet, Väino, 1952-

    2010-01-01

    Majanduskonsultant avaldab arvamust, millised on argumendid Eesti Energia kiireks börsiletulekuks, kes sellega võidaksid ja kes kaotaksid ning miks pole praegu õige aeg Eesti Energia aktsiate avalikuks müügiks

  18. Master Minimum Equipment List

    Data.gov (United States)

    Department of Transportation — The AEG System is used to create, revise, approve, and distribute text of the MMEL and Flight Standard Board (FSB)/Type Rating Report. The MMEL specifies under what...

  19. Investor interest returning as economies start to stabilize

    Index Scriptorium Estoniae

    2009-01-01

    Praegu on õige aeg Baltimaadesse investeerimiseks, kuna ka sealne majandus on stabiliseerumas. Võimalik, et Baltimaade valitsused müüvad veel riigivara. East Capital Groupi osalused Baltimaade ettevõtetes

  20. 3 vahvat / Margit Kilumets, Mari Öö Sarv

    Index Scriptorium Estoniae

    Kilumets, Margit, 1964-

    2014-01-01

    Aivo Lillest, kes on kandnud hoolt kvaliteetsete jalgrattateede rajamise eest Kalamajas; Laura Tederist, kelle hoole all on aeg-ajalt ühe perekonna viis last, ning Tambet Talist ja Kaisa Üprus-Talist, kes tegelevad leinalaagrites lastega

  1. Pop / Tõnu Pedaru

    Index Scriptorium Estoniae

    Pedaru, Tõnu

    2007-01-01

    Heliplaatidest: Ska Factor "Aeg on uus", M83 "Digital Shades. Vol 1", Keyshia Cole "Just Like You", Scandinavian Music Group "Missä Olet Laila?", Mick Jagger "The very best of Mick Jagger", Dave Gahan "Hourglass"

  2. Parliamentarians call for resignation of Finance Minister / Monika Hanley

    Index Scriptorium Estoniae

    Hanley, Monika

    2008-01-01

    Opositsiooni kuuluv erakond Uus Aeg leiab, et Läti rahandusminister Atis Slakteris peaks ebakompetentsuse tõttu tagasi astuma. Poliitikute arvates, ei ole Slakteris piisavalt aktiivselt püüdnud riigi finantsprobleeme lahendada

  3. Jah, tõesti, nii - ja mis siis? / Karlo Funk

    Index Scriptorium Estoniae

    Funk, Karlo, 1971-

    2000-01-01

    Mõtteid Eesti filmide nädalast Kinomajas ja seal näidatud Eestis 1999.aastal valminud filmidest, lähemalt Heini Drui dokumentaalfilmist "Tulekummardajad" ja Dorian Supini dokumentaalfilmist "Armastuse aeg"

  4. Kreisid ideed nõuavad pidevalt lisa / Mihkel Truman ; intervjueerinud Kadri Haavajõe

    Index Scriptorium Estoniae

    Truman, Mihkel

    2009-01-01

    Intervjuu novembri alguses 2008 Rakvere linna kultuuriosakonna juhataja kohale asunud Mihkel Trumaniga kultuurielust Rakveres, ansambli Rock-Hotel 30. juubeliks ilmunud raamatust "Aeg meid muutnud on", mille autoriks on intervjueeritav ning ajakirjast "Värske Rõhk", mille kolleegiumisse ta kuulub

  5. Muinasjutt ökokatastroofist / Ilona Martson

    Index Scriptorium Estoniae

    Martson, Ilona, 1970-

    2002-01-01

    Tallinnas ja Tartus tuli ekraanile USA joonisfilm "Jääaeg" ("Ice Age"), mille dubleerimisse on režissöör Janno Põldma juhtimisel kaasatud head eesti näitlejad Anu Lamp, Lembit Ulfsak ja Marko Matvere

  6. USA-s jagati kirjandusauhindu / Kaarel Kressa

    Index Scriptorium Estoniae

    Kressa, Kaarel, 1983-

    2007-01-01

    Ameerika Ühendriikide kirjandusauhinna National Book Award laureaadid 2007. a.: parim prosaist - Denis Johnson (romaan "Suitsupuu"), parim noorsookirjanik - Sherman Alexie ("Poole kohaga indiaanlase absoluutselt tõene päevik"), parim luuletaja - Robert Hass (luulekogu "Aeg ja ained")

  7. Muusika / Priit Kuusk

    Index Scriptorium Estoniae

    Kuusk, Priit, 1938-

    2005-01-01

    Muusikasündmustest Eestis: Improvizz'05, Jazz'n Motion, muusikafestivali "Eduard Tubin ja tema aeg" pressikonverents, laulumäng "Suur Munamägi" Estonia kammersaalis, modernismi pidunädal Tartu Elleri koolis

  8. Hetk enne plahvatust : arhitektuuri tulevik / Alberto Campo Baeza

    Index Scriptorium Estoniae

    Campo Baeza, Alberto

    1997-01-01

    Teemad: Arhitektuuri tulevik on ideedes. Valgus ja gravitatsioon : küsimuse olemus. Inimene ja tabatud ilu. Aeg ja arhitektuur. Projektiliialdused ja tehnoloogia ebaadekvaatne rakendamine. Ühiskond ja kunstnik.

  9. Territoriaalplaneerimise õiguslik regulatsioon Eesti Vabariigis : [bakalaureusetöö] / Andrus Kattel ; Tartu Ülikool, õigusteaduskond ; juhendaja: Sirje Tael

    Index Scriptorium Estoniae

    Kattel, Andrus

    1998-01-01

    Kaasaegse territoriaalplaneerimise kujunemine, Eesti Vabariigi 1939. a. ehitusseadus, nõukogude aeg, üleminekuperiood 1989-1995, 1995. a. planeerimis- ja ehitusseadus (selle asend Eesti õigussüsteemis, kohalike omavalitsuste õigused ja kohustused, mõju maakasutusele)

  10. Hea nali võib peituda raamatus / Gert Kiiler

    Index Scriptorium Estoniae

    Kiiler, Gert

    2007-01-01

    Arvustus: Leer, Jaak. Savumees Einaril on kogu aeg buss katki. Tallinn : Jutulind, 2006 ; Huber, Markus, Treichler, Robert. Keegi pole nii vinge kui meie : uimased britid, tobedad sakslased, pinges prantslased ja veel 36 lootusetut juhtumit. Tallinn : Olion, 2006

  11. "Impact 4" Berliinis ja Poznanis / Vappu Thurlow

    Index Scriptorium Estoniae

    Thurlow, Vappu, 1960-

    2005-01-01

    5.-10. IX Berliinis ja Poznanis toimunud neljandast rahvusvahelisest graafikakonverentsist "Impact" alapealkirjaga "Kontakt". Eestist osalesid Loit Jõekalda, Eve Kask, Karin Laansoo ja Vappu Thurlow. 2007. a. Tallinnas toimuva "Impact 5", alapealkirjaga "Slices of Time/Lõigutud aeg", kontseptsioon

  12. Proshtshai, dollar? / Kenneth Rogoff ; tõlk. Irina Sashtshenkova

    Index Scriptorium Estoniae

    Rogoff, Kenneth

    2008-01-01

    Autor kirjutab dollari nõrgenemisest ja selle alternatiividest rahvusvahelisel valuutaturul ning euro tugevnemisest. Pankurid ja rahandustegelased peaksid hakkama mõtlema, mida teha siis, kui on aeg dollari toetamine lõpetada

  13. Adamkus : EU might be willing to negotiate on postponing Ignalina reactor closure

    Index Scriptorium Estoniae

    2008-01-01

    President Valdas Adamkus teatas võimalusest, et EL alustab arutelu Ignalina tuumaelektrijaama sulgemise edasilükkamisest. Uue jaama valmimise aeg pole teada ning praegune reaktor on tänu rootslaste abile turvaline

  14. Raamatureisid Veneetsiasse / Margus Haav

    Index Scriptorium Estoniae

    Haav, Margus, 1969-

    2005-01-01

    Arvustus: Girardi, Robert. Vaporetto 13 / ingl. k. tlk. Karin Suursalu. Tallinn : Kultuurileht, 2005. (Loomingu Raamatukogu) ; Raittila, Hannu. Canal Grande : [romaan] / tõlkinud Aivo Lõhmus. Tallinn : Varrak, 2003 (Moodne aeg)

  15. Jõustruktuurid tunnustasid parimaid töötajaid / Kadi Laid

    Index Scriptorium Estoniae

    Laid, Kadi, 1976-

    2007-01-01

    Eesti Piirivalve tähistas 85. aastapäeva, Politsei ja Kaitseliit 89. aastapäeva. Tublimatele andsid tänukirjad üle Politseiameti peadirektor Raivo Aeg, Piirivalveameti peadirektor Roland Peets ja kaitseminister Jaak Aaviksoo

  16. Mähkmetest igaviku läveni / Aarne Ruben

    Index Scriptorium Estoniae

    Ruben, Aarne, 1971-

    2008-01-01

    Urmas Eero Liivi dokumentaalfilm "Tervitusi Nõukogude Eestist!" vabadusvõitlejatest Lagle Parekist, Tunne Kelamist ja Tiit Madissonist : RUUT, 2007. Katrin Lauri dokumentaalfilm luuletaja Debora Vaarandist "Debora Vaarandi aeg" : Content Provider 2007

  17. Tardunud pilguga / Tiia Kõnnusaar

    Index Scriptorium Estoniae

    Kõnnusaar, Tiia, 1965-

    2004-01-01

    Kuidas mõjutab telerivaatamine lapse arengut. Aluseks Saksamaa antroposoofilise suunaga Pedagoogika-, Meelte- ja Meediaökoloogia Instituudi juhataja Rainer Patzlaffi raamat "Tardunud pilk". Lisaks "Elu võimalikkusest ilma telekata" ja "Kuhu kulub aeg?"

  18. Astrocyte elevated gene-1 induces breast cancer proliferation and invasion through upregulating HER2/neu expression

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xin; ZHANG Ning; ZHANG Mei-xin

    2011-01-01

    Background Astrocyte elevated gene-1 (AEG-1),primarily identified as a late response gene induced by HIV-1 infection,plays multiple roles in the process of oncogenesis.This novel gene has been demonstrated to be involved in the several potent carcinogenic pathways,including PI3K/Akt pathway,nuclear factor (NF)-KB pathway,and Wnt/β-catenin pathway.Although the function of AEG-1 has been intensively investigated in recent years,the molecular mechanism underlying its oncogenic role is largely unknown.The aim of this research was to explore the potential function of AEG-1 in breast cancer development and progression.Methods AEG-1 was ectopically overexpressed in breast cancer MCF-7 cells and its biological effects on the proliferation and invasion of MCF-7 cells were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and invasion assays.The expression of HER2/neu,a crucial oncogene involving in breast cancer carcinogenesis,was also determined.Results Overexpression of the AEG-1 promoted the proliferation and invasion ability of breast cancer cells,and upregulated the expression of HER2/neu,a crucial oncogene involving in breast cancer carcinogenesis.Conclusion AEG-1 might facilitate the proliferation and invasion of breast cancer cells by upregulating HER2/neu expression,which provides a potential target for breast cancer therapy.

  19. Echinochrome A Improves Exercise Capacity during Short-Term Endurance Training in Rats

    Directory of Open Access Journals (Sweden)

    Dae Yun Seo

    2015-09-01

    Full Text Available Echinochrome A (Echi A improves mitochondrial function in the heart; however, its effects on skeletal muscle are still unclear. We hypothesized that Echi A administration during short-term exercise may improve exercise capacity. Twenty-four male Sprague-Dawley rats were randomly divided into the following groups: control group (CG, Echi A-treated group (EG, aerobic exercise group (AG, and aerobic exercise treated with Echi A group (AEG (n = 6 per group. Echi A was administered intra-peritoneally (0.1 mg/kg of Echi A in 300 µL phosphate-buffered saline daily 30 min before each exercise training. The AG and AEG groups performed treadmill running (20 m/min, 60 min/day five days/week for two weeks. The exercise capacity was significantly higher in the AG and AEG groups compared to other groups. Interestingly, the exercise capacity increased more effectively in the AEG group. The body weight in the EG tended to be slightly lower than that in the other groups. There were no significant changes in the plasma lipids among the groups. However, the gastrocnemius muscle mitochondria content was greater in the EG and AEG groups. These findings show that Echi A administration after short-term endurance training enhances exercise capacity, which was associated with an increase in skeletal muscle mitochondrial content.

  20. Six Key Topics for Automated Assessment Utilisation and Acceptance

    Directory of Open Access Journals (Sweden)

    Torsten REINERS

    2011-04-01

    Full Text Available Automated assessment technologies have been used in education for decades (e.g., computerised multiple choice tests. In contrast, Automated Essay Grading (AEG technologies: have existed for decades; are `good in theory' (e.g., as accurate as humans, temporally and financially efficient, and can enhance formative feedback, and yet; are ostensibly used comparatively infrequently in Australian universities. To empirically examine these experiential observations we conducted a national survey to explore the use of automated assessment in Australian universities and examine why adoption of AEG is limited. Quantitative and qualitative data were collected in an online survey from a sample of 265 staff and students from 5 Australian universities. The type of assessment used by the greatest proportion of respondents was essays/reports (82.6%, however very few respondents had used AEG (3.8%. Recommendations are made regarding methods to promote technology utilisation, including the use of innovative dissemination channels such as 3D Virtual Worlds.

  1. Geant4 Simulation of Annihilation and excitation of Positronium

    CERN Document Server

    Gad, Andreas

    2016-01-01

    The purpose of this report is to document the work done during the summer of 2016 (18/6- 26/8) as a part of the CERN Summer Student Programme. The work has been done at the AEgIS (Antihydrogen Experiment: Gravity, Interferometry, Spectroscopy) collaboration under the supervision of Lillian Smestad and Michael Doser. The goal of the project was to create a Monte Carlo simulation in Geant4, of Positronium annihilation and excitation in the positron test chamber of the AEgIs experiment.

  2. Igasse maaperesse viis küülikut! / Harald Tikk

    Index Scriptorium Estoniae

    Tikk, Harald, 1931-

    2003-01-01

    Kuna praegune aeg on maaelus üsna keerukas, näeb autor küülikukasvatust tootmisharuna, mis peaks olema igale maaperele jõukohane. Ülevaade sellest, millele peaks tähelepanu pöörama küülikukasvatusega alustades

  3. Valgaskii tribunal / Josef Kats

    Index Scriptorium Estoniae

    Kats, Josef, 1977-

    2004-01-01

    Ilmunud ka: Narvskaja Gazeta, 27. mai 2004, lk. 8. Eesti peaministri, Res Publica esimehe Juhan Partsi ja Läti ekspeaministri, partei Uus Aeg juhi Einars Repshe ühisavaldusest Euroopa Parlamendile, milles nõudsid kommunismi kuritegelikuks kuulutamist ning Venemaalt okupatsiooniaegsete kuritegude avalikku tunnistamist. Poliitikute kohtumisest Valgas

  4. Lapsesamm Trullist Dostojevskini / Vaapo Vaher

    Index Scriptorium Estoniae

    Vaher, Vaapo, 1945-

    2011-01-01

    Tutvustus: Raitar, Maie ; Sööt, Andres. Konrad Mägi. Kunstnik. Looming. Aeg. Tartu : Ilmamaa, 2011 ; Fitzgerald, F. Scott. Maipüha. Tallinn : Eesti Raamat, 2011 ; Trull, Ilmar. Väike viisakas kärbes. Tallinn : Varrak, 2011

  5. Mart Laar tuleb peatada / Villu Reiljan

    Index Scriptorium Estoniae

    Reiljan, Villu, 1953-

    2001-01-01

    Ilmunud ka: Vooremaa, 8. dets. 2001, lk. 2; Nädaline, 8. dets. 2001, lk. 4; Võrumaa Teataja, 11. dets. 2001, lk. 2; Järva Teataja, 13. dets. 2001, lk. 2. Kuigi ca 70% Eesti rahvast on elektrijaamade erastamise vastu, on Mart Laari valitsus ignoreerinud rahva tahet, talle on kogu aeg sekundeerinud Mihkel Pärnoja. Autor: ERL. Parlamendisaadik

  6. Estonskii film prinimali teplo / Marianne Kõrver

    Index Scriptorium Estoniae

    Kõrver, Marianne, 1980-

    2006-01-01

    Lühimängufilm "Tühirand", režissöör Veiko Õunpuu, osales Karlovy Vary rahvusvahelise filmifestivali "Forum of Independents" programmis. 41. Karlovy Vary filmifestivali avas Korea režissööri Kim Ki-duki uusim film "Aeg". Lühidalt ka auhinnatutest ja huvitavamatest filmidest

  7. Eesti filmil läks hästi Karlovy Vary festivalil / Marianne Kõrver

    Index Scriptorium Estoniae

    Kõrver, Marianne, 1980-

    2006-01-01

    Lühimängufilm "Tühirand", režissöör Veiko Õunpuu, osales Karlovy Vary rahvusvahelise filmifestivali "Forum of Independents" programmis. 41. Karlovy Vary filmifestivali avas Korea režissööri Kim Ki-duki "Aeg". Lühidalt ka auhinnatutest ja huvitavamatest filmidest

  8. Kange prooviga Eesti kuld / Kristel Kossar

    Index Scriptorium Estoniae

    Kossar, Kristel

    2008-01-01

    Marina Kaljurannal täitub juulis kolm aastat suursaadikuna Venemaal, see aeg tõi talle mullu Eesti aasta inimese lugupidava tiitli. Marie Claire veetis temaga Moskvas ühe päeva, et tuua tükike suursaadiku elust miljonilinnas oma lugejateni

  9. [Ilukirjandus ainetundi ja raamat korvpalliplatsile] / Edward Kess

    Index Scriptorium Estoniae

    Kess, Edward

    2009-01-01

    2010. aasta on kuulutatud lugemisaastaks. Raplamaal aset leidnud Eesti Emakeeleõpetajate Seltsi ja Kultuuriministeeriumi ühisseminarist "Lugemisaasta - lugemise aeg". Aruteludes koorus välja tähelepanek, et kõikide ainete õpetajad peaksid kasutama oma tundides ilukirjandust, et pakkuda õpilastele vaheldust õpiku-töövihiku tunnile

  10. Mis on kuuekümnendad eesti kirjanduses? / Mart Velsker

    Index Scriptorium Estoniae

    Velsker, Mart, 1966-

    1999-01-01

    Erinevustest lääne ja eesti kirjanduses 1960. a-tel. Ka poleemikast Tiit Hennoste loengusarjaga "Hüpped modernismi poole. Eesti 20. sajandi kirjandusest Euroopa modernismi taustal". Ettekanne Tartu Kirjanduse Majas 1999. a. mais toimunud Soome-Eesti ühisseminaril. Ilmunud ka kogumikus: Taasleitud aeg = Kadonneen ajan arvoitus. Tartu, 2000, lk. 51-59.

  11. Töötajad hoolitsevad ise otstarbeka ajakasutuse eest / Merike Lees

    Index Scriptorium Estoniae

    Lees, Merike, 1976-

    2007-01-01

    Ilmunud ka: Delovõje Vedomosti 28. nov. lk. 38. Andes töötajatele vabaduse ise otsustada oma tööaja üle, paranevad nende töö efektiivsus ning ettevõtte majandustulemused. Lisa: Paindlik tööaeg toob kasu. Kommenteerib Aneta Jajkowska

  12. Mõtteid isast ja ta ajast / Enn Soosaar

    Index Scriptorium Estoniae

    Soosaar, Enn, 1937-2010

    2006-01-01

    6. septembril möödub sada aastat praost Albert Soosaare (1906-1995) sünnist. Järgneb: 6. september, lk. 6; 13. september, lk. 6; 20. september, lk. 6; 27. september, lk. 6; 4. oktoober, lk 6; 11. oktoober, lk 6. Katkendid pärinevad pikemast esseest "Isa ja Aeg"

  13. Research into the Bronze and Early Iron Ages / Aivar Kriiska

    Index Scriptorium Estoniae

    Lang, Valter, 1958-

    2006-01-01

    19. sajandil olid mõisted pronksiaeg ja eelrooma rauaaeg praktiliselt tundmatud. Kuni 1950ndateni domineeris rooma rauaaja uurimine. Nüüd on selgunud, et seni kultuurilise depressiooni perioodiks peetud aeg (tuntud kivikirstkalmete perioodina), oli tegelikult üks dünaamilisemaid perioode Eesti esiajaloos. Eesti ala ebaühtlasest asustusest sel perioodil

  14. ERGO Jazz Session võõrustab Chris Gall Triot. Vennad Urbid ajakohaste lauludega

    Index Scriptorium Estoniae

    2008-01-01

    14. septembril alustab Jazzkaar koostöös ERGO Kindlustusega uut sarja ERGO Jazz Session, mille avaesinejaks on Chris Gall Trio (kontserdid 14. sept.Kumu auditooriumis ja 15. sept. Tartus Athena keskuses). Vendade Urbide kontserdist "Aeg" 13. sept. Vanemuise kontserdimajas

  15. Kill a brand, keep a customer / Nirmalya Kumar

    Index Scriptorium Estoniae

    Kumar, Nirmalya

    2004-01-01

    Autori hinnangul peaksid kõik ettevõtted kasumi suurendamiseks aeg-ajalt oma brändiportfelle optimeerima. Brändide hindamisest, liitmisest, müümisest, kasvatamisest ja kaotamisest. Tabel: The brand audit sheet. Lisa: Do you have too many brands?

  16. Kunstnike uus põlvkond Kumus / Ragne Nukk

    Index Scriptorium Estoniae

    Nukk, Ragne

    2008-01-01

    Eesti Kunstiakadeemia vabade kunstide osakonna magistrantide lõputööde näitusest Kumu kunstimuuseumis. Pikemalt kunstnikeduo Johnson&Johnson dokumentatsioonist, Marge Monko fotoseeriast "Uurimusi kodanlusest", Leen Võrno tööst "Aeg/koht, ajatus/kohatus", mis on kujundus lühifilmile. Näituse kuraator Maarin Ektermann

  17. Pathways through which health influences early retirement: A qualitative study

    NARCIS (Netherlands)

    A. de Wind (Astrid); G.A. Geuskens (Goedele); K.G. Reeuwijk (Kerstin); M.J. Westerman (Marjan); J.F. Ybema (Jan Fekke); A. Burdorf (Alex); P.M. Bongers (Paulien); A.J. van der Beek (Allard)

    2013-01-01

    textabstractBackground: Due to the aeging of the population, there is a societal need for workers to prolong their working lives. In the Netherlands, many employees still leave the workforce before the official retirement age of 65. Previous quantitative research showed that poor self-perceived heal

  18. O sudbe "Estonii" sprosite Laara / Max Kaur

    Index Scriptorium Estoniae

    Kaur, Max, 1969-

    2007-01-01

    Autor leiab, et on tulnud aeg, mil Estonia laevahuku asjus peaksid Mart Laar ja teised Eesti tolleaegsed juhid andma ausaid ja avameelseid tunnistusi; Estonia huku uurimiseks tuleks luua EL-i ja NATO egiidi all uus uurimisrühm, kus peale Eesti, Rootsi ja Soome esindajate oleksid ka Venemaa esindajad

  19. Kolmanda raamatu kiituseks / Peeter Olesk

    Index Scriptorium Estoniae

    Olesk, Peeter, 1953-

    2009-01-01

    Tutvustus: Isaacson, Walter. Einstein : tema elu ja universum / tõlkinud Matti Piirimaa. Tallinn : Eesti Raamat, 2009; Fortey, Jacqueline. Kuulsad teadlased (Silmaring) / tõlkinud Ülle Haav ja Helen Haav. Tallinn : Koolibri, 2009; Vaim ja aeg (Lehed ja tähed : looduse ja teaduse aastaraamat, 5) / koostanud Indrek Rohtmets ja Toomas Kukk. Tallinn : Loodusajakiri, 2009

  20. Hirmu teretulnud naasmine maailma rahaturgudele / Martin Wolf ; tõlk. Erik Aru

    Index Scriptorium Estoniae

    Wolf, Martin

    2007-01-01

    Ajalehe The Financial Times majanduskommentaator finantskriisist maailmamajanduses. Tõenäoliselt saabub kahjumite kokkuarvamise, karmistuvate krediiditingimuste ja laenukoormuste vähendamise aeg, mis toob kaasa tugeva surve intressimäärade järsuks langetamiseks, et kriisiohtlik süsteem hädast päästa. Sellele survele tuleks vastu hakata nii kaua kui võimalik

  1. Allkirjade alkeemiast / Maie Kiisel

    Index Scriptorium Estoniae

    Kiisel, Maie

    2011-01-01

    Autori arvates hakkab vanamoodsate kodanikualgatuste aeg läbi saama, tõusulainel on aga Internetis allkirjade jagamine. GMO-keelu tühistamise vastu koguti 1,2 mln. allkirja. Autor leiab, et liikmesriigid on Euroopa tasandi kodanikualgatust kartma hakanud

  2. Kogemus kui ajalugu / David Carr ; inglise keelest tõlkinud Triinu Pakk ; toimetanud Tõnu Viik

    Index Scriptorium Estoniae

    Carr, David, 1940-

    2012-01-01

    Representatsioon, mälu, kogemus. Ajalisus. Intentsionaalsus ja loomuliku hoiaku maailm. Inimmaailm ja ajalooline maailm. Sotsiaalne aeg ning laiendatud minevik ja tulevik. Osalus ja liikmelisus. Ajaloolisus. Ajaloolisus ja ajalooline teadmine. Teaduse ajaloolisus. Ajaloo metafüüsika ja epistemoloogia.

  3. Kas kolm ikka on koolipoisi number? / Mare Leino

    Index Scriptorium Estoniae

    Leino, Mare, 1961-

    2009-01-01

    Artiklikogumiku ""Probleemsed" poisid või "vale" temperament?" (Tallinn, 2009) tutvustus. Kogumik osutab, et tänapäeva kool sobib tüdrukutele paremini kui poistele. On aeg hakata senisest põhjalikumalt uurima, miks see nii on

  4. Teenitud vanaduspuhkus

    Index Scriptorium Estoniae

    2006-01-01

    EPL arvab, et Kuna president Arnold Rüütel tervitas vabariigi aastapäeva paraadil kaitseväelasi võidupüha puhul ning avaldas pärast teleesinemist kaastunnet ETV toimetajale, pidades teda ekslikult Helle Meri'ks, siis oleks tal aeg siirduda välja teenitud vanaduspuhkusele

  5. Novaja jadernaja ugroza / Louis-Rene Beres

    Index Scriptorium Estoniae

    Beres, Louis-Rene

    2007-01-01

    Tänapäeval võib terroristide käsutuses olla nii tuumarelv kui ka teisi massihävitusrelvi ning sellest tingituna on vaja ka uut julgeolekustrateegiat. On tulnud aeg välja töötada kaasaegne Manhattani projekti analoog

  6. E-citizen in an E-state / Tarmo Loodus

    Index Scriptorium Estoniae

    Loodus, Tarmo, 1958-

    2001-01-01

    Kaanel ajak. ilmumis aeg Winter 2001/2002. Eesti peab muutuma riigiks, kus avaliku sektori asjaajamine toimub ööpäevaringselt, Interneti-ühendus koduarvuteis on tavaline, kasutusele võetakse digitaalallkiri - kõik see nõuab lisaks tehnoloogia arengule ka organisatoorseid ümberkorraldusi. Autor: Isamaaliit

  7. Individual concrete panels

    DEFF Research Database (Denmark)

    2008-01-01

    DVD én formidler en workshop omhandlende udvikling, optimering og produktion af individuelle betonelementer. Workshoppen fandt sted 8. til 13. oktober 2007 i en af Peter Behren´s AEG turbinehaller i Berlin, hvor 10. arkitektstuderende fra Århus satte 10. inginiørstuderende fra tekniske universite...

  8. Päästke mööbel! / Marko Rillo

    Index Scriptorium Estoniae

    Rillo, Marko

    2006-01-01

    Näide üle-eestilise juhtimiskonkursi "Juhtimisaju 2006" ühest ülesandest ning võistkonna "Mõistuse hääl" probleemilahendus. Lisa: Ülesanne; Vt. samas: Kas tankis põlenud poiste aeg pole mitte läbi? Lisa: Teinto Furniture'i SWOT analüüs

  9. Muuga söeterminalil on probleemide lahendamiseks aega jaanipäevani / Ulvar Käärt

    Index Scriptorium Estoniae

    Käärt, Ulvar, 1982-

    2006-01-01

    AS Coal Terminal Operatori söeterminal Muugal, kust aeg-ajalt levib ümbruskonda söetolmu, töötab nii ilma välisõhu saasteloa kui ka vee erikasutusloata. Terminal kavandab meetmeid tolmuprobleemi lahendamiseks

  10. "igasse asjade ilmnemisse..." : [luuletused] / Jan Kaus

    Index Scriptorium Estoniae

    Kaus, Jan, 1971-

    2004-01-01

    Sisu: "igasse asjade ilmnemisse..." ; "õnn ja seebimull..." ; "jumala eest! küsige..." ; "aeg surub lõuad kokku..." ; "see kes täna küla naerutab..." ; "kui lips ongi läbi..." ; "klotse asetavast poisist..." ; "mulle ei jää..." ; Mattias ja öö

  11. Andrus Saar: Neid inimesi, kes peavad õhtul tühja kõhuga magama minema, on ka parimatel aegadel olnud liiga palju / Andrus Saar ; intervjueerinud Katre Ratassepp

    Index Scriptorium Estoniae

    Saar, Andrus, 1946-

    2010-01-01

    Vaesuse ja sotsiaalse tõrjutuse vastu võitlemise Euroopa aasta Eesti hea tahte saadik leiab, et hirm kaotada töökoht on igati normaalne olukorras, kus inimesed ei ole informeeritud ettevõtte arengust ja perspektiividest ning et praegu oleks viimane aeg muuta toetuste süsteemi. Saar Polli poolt läbiviidud Eurobarometer Flash uuringust

  12. Kolmekihiline Kopli tort Märjamaal / Sulev Oll

    Index Scriptorium Estoniae

    Oll, Sulev, 1964-

    2010-01-01

    Marje Jurtšenko dokumentaalfilmist "Aeg on siin", mis räägib aastast Eesti eri paikades elava nelja 7aastase lapse elus. Filmi esilinastas filmis osalenud Fredile ja tema koolikaaslastele Märjamaa kinos Helk kinomehhaanik Hans Veimann

  13. Töötuskindlustushüvitise suurus ületab kindlustusmaksed mitmekordselt / Anne Lauringson

    Index Scriptorium Estoniae

    Lauringson, Anne

    2008-01-01

    Ilmunud ka: Narvskaja Gazeta : Töötukassa 27. märts lk. 4, Den za Dnjom : Töötukassa 28. märts lk. 4. Töötuskindlustushüvitise eesmärgist ja saamise võimalustest. Diagramm: Hüvitise saamise aeg kuudes

  14. Vtoroje dõhanije / Galina Balashova

    Index Scriptorium Estoniae

    Balashova, Galina

    2001-01-01

    Ehtekunstnik Jaan Pärna projektist Meistrite hoov. Dolomiidi kasutamist kunstis kajastavast näitusest "Pae aeg" Meistrite hoovi galeriis. Kujundas Urve Küttner. Jaan Pärna, Urve Küttneri, Andrei Balashovi ja Helmet Raja töödest näitusel

  15. Kroonika - chronicle (2006)

    Index Scriptorium Estoniae

    2007-01-01

    Helilooja Eduard Tubina helitööde ettekannete, raamatute, artiklite, helisalvestiste nimekiri. Dokumente ja väljavõtteid artiklitest, mis sisaldavad Vardo Rumesseni kirju peaministrile, kultuuriministrile ja BNS teadet dirigent Neeme Järvi kriitilisest suhtumisest kultuuriministeeriumisse seoses festivaliga "Tubin ja tema aeg"

  16. USA hiiglane GE Healthcare korraldab meditsiinis revolutsiooni / Andrew Jack

    Index Scriptorium Estoniae

    Jack, Andrew

    2006-01-01

    General Electricu tütarettevõte GE Healthcare võttis üle Briti diagnostika- ja bioteaduse uurimisfirma Amersham, firma strateegia tuum on võimsa skaneerimistehnoloogia ja meditsiinilise diagnostika üksteisele lähendamine. Lisa: Aeg sorteerida andmeid infotehnoloogia abil

  17. Uus kunstikool näitab oma nägu ja hambaid / Karen Jagodin

    Index Scriptorium Estoniae

    Jagodin, Karen, 1982-

    2004-01-01

    Positivistliku Kunsttööstuskooli kampaaniapäevad algasid Performance Week'iga Ku galeriis. Avamisel esines Mari Sobolev performance'iga "Lost in the system". Kampaaniapäevad kulmineeruvad Paide tegevuskunstifestivaliga "Aeg. Ruum. Liikumine: unplugged". Festivali kuraator on Mari Sobolev

  18. Stenbocki maja õues kuuleb põnevaid kodumaiseid artiste

    Index Scriptorium Estoniae

    2008-01-01

    Stenbocki maja õuemuusika kontserdisarja kontsertidest: 18. juunil esinevad Rein Rannap ja Tiit Kikas, 2. juulil kontsert "Jazz Emotion" (Sofia Rubina), 16. juulil eesti-ukraina folkansambel Svjata Vatra, juuli eelviimasel nädalal kontsert "Tshellopalavik" (C-Jam ja Kaire Vilgats), augustis kontsert "Hea-tuju-laulud" (Revalia kammermeeskoor), 20. aug. kontsert "Aeg ei peata" (esitusel Ott Arderi sõnadele loodud laulud)

  19. Эстонии пора думать о новом массовом строительстве жилья / Урмас Тооминг

    Index Scriptorium Estoniae

    Тооминг, Урмас, 1954-

    2011-01-01

    Eestis 1960–1990 ehitatud kortermajade eluiga hakkab paarikümne aasta pärast otsa saama. Aeg on algatada massilise elamuehituse käivitamise teemal mõttetalguid, et tulevikus vältida keerulist eluasemeprobleemi. Probleemi lahkavad MTÜ Eesti Arhitektuurikeskuse juhataja Ülar Mark ja Tallinna peaarhitekt Endrik Mänd

  20. Rohelisest linnast : haljas linn / Ignar Fjuk

    Index Scriptorium Estoniae

    Fjuk, Ignar, 1953-

    2006-01-01

    Linnahaljastusest Eestis, haljastuse tähtsusest linnaplaneerimises, ajaloost ja tänapäevast. Näiteks toodud Tallinna vanalinna Harju t. äärde kavandatav haljasala, mis eeldaks projektivõistlust, Kadrioru pargi alumises aias elujõuliste puude hävitamise kava jm. Autori arvates oleks aeg haklata rajama 21 sajandi parke

  1. Bill Clinton: enne surma tahaks käia ära Kilimanjarol / Kaivo Kopli

    Index Scriptorium Estoniae

    Kopli, Kaivo

    2010-01-01

    USA endine president Bill Clinton peab end juba nii vanaks, et oleks aeg hakata koostama nimekirja viimastest soovidest, mis siin ilmas veel täituda võiks. Näiteks soovib ta ronida Kilimanjaro mäele ning joosta maratoni

  2. AcEST: BP913198 [AcEST

    Lifescience Database Archive (English)

    Full Text Available og 1 OS=Aedes aeg... 48 2e-05 sp|Q9H4E7|DEFI6_HUMAN Differentially expressed in FDCP 6 homolog... 48 2e-05 s...47 4e-05 sp|Q8C2K1|DEFI6_MOUSE Differentially expressed in FDCP 6 OS=Mus ... 47 5

  3. Eesti sihiks elukvaliteedi kasv / Mait Raava

    Index Scriptorium Estoniae

    Raava, Mait

    2007-01-01

    Autor näeb Eesti põhiprobleemi selge visiooni puudumises ning leiab, et sihiks tuleks võtta elukvaliteedi stabiilne kasv. Eestis on aeg küps selleks, et parteid tuleksid järgmisteks valimisteks välja järgmise 15-30 aasta visiooniga

  4. Tallinn Fashion Week täidab nädala kodumaise moega / Karilin Engelbrecht

    Index Scriptorium Estoniae

    Engelbrecht, Karilin

    2009-01-01

    Tallinn Fashion Week'i üritustest. Ülevaade eesti moekunstnike loomingu esitlustest. Näitus "Uus Eesti moejoonistus" Arhitektuuri- ja Disainigaleriis. Anu Hindi moefotonäitus "Aeg maha 3" Iida galeriis. Raamatu "Eesti moepiibel 2010" esitlus Rotermanni kvartali kohvikus Platz. Oksana Tanditi ja Filippo Caroti moefotonäitus "Butterflies" Tallinna botaanikaaias

  5. Näitused / Mari Sobolev

    Index Scriptorium Estoniae

    Sobolev, Mari, 1968-

    1998-01-01

    Tartu Kunstimaja: "Autor x autor"; Vaal-galerii: Mall Nukke "Mängude aeg"; Linnagalerii Tallinnas "Paldiski lood"; Kastellaanimaja: Tiit Pääsuke "Varjab ja valgustab"; Draakoni galerii: leedu kunstniku Nele Zirnite graafikanäitus "Aja märk"; La galerie passage: "Ühised algused".

  6. Alar Pääro La Galerie Passage'is / Viivi Põlma

    Index Scriptorium Estoniae

    Põlma, Viivi

    2000-01-01

    Iseõppijast maalikunstniku Alar Pääro esimene personaalnäitus "On aeg" 20. I-20. II La Galerie Passage'is. 1993. aastast on A. Pääro töötanud vabakutselise kunstnikuna, mida on talle võimaldanud metseen Kalev Klais

  7. IPO. Mäng suure orkestriga / Tiiu Pedaja, Gerd Laub

    Index Scriptorium Estoniae

    Pedaja, Tiiu

    2006-01-01

    Aktsiate avaliku pakkumise ehk IPO korraldamisest. Lisad: Millal on õige aeg?; Milline ettevõte sobib avalikuks pakkumiseks?; Milline avalik pakkumine on edukas?; Kuidas IPO-l aktsiaid jaotatakse? Skeemid: Aktsia hinna kujunemine avaliku pakkumise käigus; Järelturu aktsiahinda mõjutavad tegurid

  8. Prantsusmaa silub Ida-Euroopga suhteid / Riina Luik

    Index Scriptorium Estoniae

    Luik, Riina, 1962-

    2004-01-01

    Prantsusmaa peaminister Jean-Pierre Raffarin kohtus Pariisis Ida-Euroopa ajakirjanikega ja rääkis EL-i riikide suhetest ja majanduskoostööst. Vt. samas: Andres Kasekamp. Näpuga viibutamise aeg on möödas. Lisa: Jean-Pierre Raffarin (55)

  9. Eestlased Peipsi taga mõisavalitsejaks / Heli Raamets

    Index Scriptorium Estoniae

    Raamets, Heli, 1975-

    2008-01-01

    AS Trigon Agri Heiki Volveri juhtimisel rajab Pihkva oblastisse Gdovi linna lähedal asuvasse Dobrutshi külla kaasaegset piimafarmi, projekti maksumuseks on ligi 10 miljonit eurot. Vt. samas: Venemaal kestab Vene aeg edasi; Trigon Agril kolmes riigis 35 põllumajandusettevõtet

  10. Sobõtinoje vremja / Dmitri Mihhailov

    Index Scriptorium Estoniae

    Mihhailov, Dmitri

    2003-01-01

    Mõiste "aeg" füüsikalisest ja filosoofilisest tõlgendamisest. Nikolai Kozõrevi "põhjusliku mehhaanika" teooriast. Leibniz'i, Deleuze'i ja Whitehead'i aja käsitlustest. Vana-Kreeka ajajumalatest - Kronosest ja Aionist, nende vastandlikkusest ja ühtsusest

  11. Ilmusid Rahvusraamatukogu uuenenud toimetised / Mihkel Volt

    Index Scriptorium Estoniae

    Volt, Mihkel

    2009-01-01

    Tutvustus: Ajalookirjutaja aeg = Aetas historicorum / Eesti Rahvusraamatukogu ; koostanud Piret Lotman. (Eesti Rahvusraamatukogu toimetised, nr. 11). Tallinn, 2008 ; Teenuse kvaliteet – raamatukogutöö tulemuslikkuse näitaja = Service quality – library perfomance indicator / Eesti Rahvusraamatukogu ; koostanud Anne Veinberg. (Eesti Rahvusraamatukogu toimetised ; 12). Tallinn, 2009.

  12. Fotod Michel Sittowi loomingust

    Index Scriptorium Estoniae

    2001-01-01

    Nigulistes Michel Sittowi 475. surma-aastapäevale pühendatud õppeotstarbeline reprofotode näitus kunstniku loomingust. Koostaja Helena Risthein, kujundaja Liina Siib, fotod - Stanislav Stepashko. 20. I teabepäev ajaloo- ja kunstiõpetajatele "Michel Sittow ja tema aeg". Kava vt. lk. 22. Kunstnikust.

  13. [Raamatud] / Teet Korsten

    Index Scriptorium Estoniae

    Korsten, Teet, 1967-

    2006-01-01

    Tutvustus: Murakami, Haruki. Norra mets : romaan / jaapani keelest tõlkinud Kati Lindström. Tallinn : Varrak, 2006. (Moodne aeg) ; Naised on nii imelised : [luulekogumik / koostanud ja järelsõna kirjutanud Jaak Urmet. Tallinn] : Tänapäev, 2006

  14. Experience in developing control integrated multilevel systems for gas transport; Developpement de systemes integres de gestion multi-niveaux pour le transport du gaz

    Energy Technology Data Exchange (ETDEWEB)

    Kostyukov, V.Y. [NIIIS, (Russian Federation); Bityukov, V.S. [Gasprom, (Russian Federation)

    2000-07-01

    This report describes the experience of the integrated control multilevel system (IACS) development and implementation for gas transport at the regional enterprises of JSC 'Gasprom', specificity of IACS creation by the Russian enterprises on the basis of the technical and licensed basic software SCADA Geamatics purchased from AEG company under the contract. (authors)

  15. Keskmise suurusega akutrellid / Rakennusmaailma põhjal Ingrid Maasik

    Index Scriptorium Estoniae

    2011-01-01

    Võrdluses on 14,4 V akutrell-kruvikeerajad: AEG BS14 C; Black&Decker HP146F4L; Bosch GSR 14,4 V-LI; Bosch PSR LI-2; Einhell RT-CD; Festool T 15+3; Hitachi DS 14BDL; Makita BDF442; Metabo LT; Milwaukee C14DD; Panasonic EY 7441LR; Spit SDI 145

  16. Mind häirib edukultus. Mind häirib progressikultus. Mind kohutavalt häirib laristamine ja raiskamine / Enn Soosaar ; interv. Urmo Soonvald

    Index Scriptorium Estoniae

    Soosaar, Enn, 1937-2010

    2007-01-01

    Tõlkija, esseist ja kolumnist räägib poliitikast, Eesti ühiskonnast, Eesti suhetest Venemaaga, oma kokkupuudetest presidentide Lennart Meri, Arnold Rüütli ja Toomas Hendrik Ilvesega. Vt. samas: President Ilves tsiteerib 24. veebruaril 2007 Soosaart. Vabariigi aastapäeva kõnes tsiteeris president T. H. Ilves E. Soosaare raamatut "Isa ja Aeg"

  17. Eesti tippnäitlejad andsid multikale hääle

    Index Scriptorium Estoniae

    2002-01-01

    Esimest korda dubleeritakse välismaa näitlejate hääled eesti keelde animafilmis "Jää aeg" ("Ice Age") : Ameerika Ühendriigid 2002. Dublaazhi režissöör ja tõlkija Janno Põldma, teine tõlkija Lauri Kaare

  18. Magustoit filmihuvilistele lastele / Maris Balbat

    Index Scriptorium Estoniae

    Balbat, Maris, 1936-

    2002-01-01

    25. oktoobrist tuleb Coca-Cola Plaza ekraanile USA joonisfilm "Jääaeg" ("Ice Age"), mille dubleerimisse on režissöör Janno Põldma juhtimisel kaasatud head eesti näitlejad Anu Lamp, Lembit Ulfsak, Marko Matvere jt

  19. Eesti näitlejad andsid hääle laiskloomale, mammutile ja tiigrile / Ilona Martson

    Index Scriptorium Estoniae

    Martson, Ilona, 1970-

    2002-01-01

    Neljapäeval Tallinnas kobarkinos ekraanile tuleva USA joonisfilmi "Jääaeg" ("Ice Age") dubleerimisse on režissöör Janno Põldma juhtimisel kaasatud parimad eesti näitlejad. J. Põldma filmist ja selle dubleerimisega kaasnenud seikadest

  20. Kolm jääaegset triumfi / Peep Pedmanson

    Index Scriptorium Estoniae

    Pedmanson, Peep

    2002-01-01

    Tallinnas ja Tartus tuli ekraanile USA joonisfilm "Jääaeg" ("Ice Age"), mille dubleerimisse on režissöör Janno Põldma juhtimisel kaasatud parimad eesti näitlejad. Eriti Anu Lambi meisterlikkusest laisklooma dubleerimisel ning arvutianimatsiooni kõrgest tasemest

  1. Jah-sõna Hollywoodist paneb aluse kiiduväärt tavale / Anneli Aasmäe

    Index Scriptorium Estoniae

    Aasmäe, Anneli, 1973-

    2002-01-01

    Tallinnas ja Tartus tuli ekraanile USA joonisfilm "Jääaeg" ("Ice Age"), mille dubleerimisse on režissöör Janno Põldma juhtimisel kaasatud parimad eesti näitlejad. Tallinna kobarkino pressiesindaja Lauri Kaare väidab, et see dublaazh ei jää viimaseks

  2. Budjet li polza dlja ekonomiki stranõ?

    Index Scriptorium Estoniae

    2005-01-01

    Valitsuse programmist töötajate koondamisel, tööandjate pakutud variant 14+14 (töötaja koondamise puhul kahenädalane etteütlemise aeg ja kaks nädalat kompensatsiooni). Arvamust avaldavad: Eiki Nestor, Mart Opmann, Fjodor Berman, Harri Taliga ja Vitali Burlakov

  3. Milleks on vaja (koledat) kirjandust? / Kurt, pseud Moor, pseud.

    Index Scriptorium Estoniae

    Moor, Kurt, pseud

    2004-01-01

    Sari "Moodne aeg" kirjanduslike kolmapäevade raames (toimus 27. okt. 2004). Tutvustati Bret Easton Ellise "Ameerika psühhopaati" (Jaanus Adamson), Ian McEwani "Tsementaeda" (Ene-Reet Soovik) ja Martin Amise loomingut (Valle-Sten Maiste)

  4. Halb nullvariant / Rein Taagepera

    Index Scriptorium Estoniae

    Taagepera, Rein, 1933-

    2002-01-01

    Ilmunud ka: Infopress 9. august nr. 32 lk. 34. On aeg uuesti läbi mõelda, kuidas süvendada eesti meelt ja eesti keelt 220 000 mittekodaniku hulgas. Kodakondsuseksami asemel võiks kaaluda 150-tunnise harjutuse läbimist, pakub autor. Autor: Res Publica

  5. Valmiera teater mängib Võnnu lahingut Cēsise lossivaremetes / Jaanika Kressa

    Index Scriptorium Estoniae

    Kressa, Jaanika

    2009-01-01

    Cēsise lossivaremetes etendub Viesturs Meikšānsi vabadusvõitluse teemaline lavastus "Lätlaste aeg". Lauris Gundars kirjutas näidendi Võnnu lahingu 90. aastapäevaks, tuginedes reaalsetele ajaloolistele sündmustele. Läti-Eesti ühisprojektist "Kristjan Jaak ja Eduard"

  6. Värdjatest, kui mitte muust / Karlo Funk

    Index Scriptorium Estoniae

    Funk, Karlo, 1971-

    1999-01-01

    Mängufilmid "Tantsija aeg" ("Vremja tantsora") : Stsenarist Aleksandr Mindadze : režissöör Vadim Abdrashitov : Venemaa 1997 ja "Värdjatest ja inimestest" ("Pro urodov i ljudei") : Stsenarist ja režissöör Aleksei Balabanov : Venemaa - Ameerika Ühendriigid 1998

  7. Eestlased esitlevad Veneetsias kuivkäimlat

    Index Scriptorium Estoniae

    2004-01-01

    10. IX avatakse Veneetsias Arsenales IX arhitektuuribiennaali "Metamorfoos" raames Eesti ekspositsioon "Aeg-maha-arhitektuur". Kristjan Holmi projekt tutvustab Eesti kuivkäimla arhitektuuri. Kabli rannast on näitusesaali toodud arhitekt Agu Külma ja skulptor Jukka-Pekka Jalovaara ehitatud hobusekujuline välikäimla. Näitusega kaasneb kataloog ja dokumentaalfilm Kuukulgur Filmilt

  8. Kirjastusuudiseid

    Index Scriptorium Estoniae

    2006-01-01

    eesti Kunstimuuseumi kirjastatud raamatud "Vene valitsejad. Vene valitsejate portreed Eesti muuseumide kogudest" (koostajad Mai Levin, Aleksandra Murre; kujundaja Peeter Laurits), "Eros ja Surm. Felicien Ropsi looming" (toimetaja Tiina Abel, kujundaja Mari Kaljuste) ja märkmik-kalender "Inimene ja aeg. Päevaraamat" (kujundaja Jaak Peep)

  9. Moss Pathogenesis-Related-10 Protein Enhances Resistance to Pythium irregulare in Physcomitrella patens and Arabidopsis thaliana.

    Science.gov (United States)

    Castro, Alexandra; Vidal, Sabina; Ponce de León, Inés

    2016-01-01

    Plants respond to pathogen infection by activating signaling pathways leading to the accumulation of proteins with diverse roles in defense. Here, we addressed the functional role of PpPR-10, a pathogenesis-related (PR)-10 gene, of the moss Physcomitrella patens, in response to biotic stress. PpPR-10 belongs to a multigene family and encodes a protein twice the usual size of PR-10 proteins due to the presence of two Bet v1 domains. Moss PR-10 genes are differentially regulated during development and inoculation with the fungal pathogen Botrytis cinerea. Specifically, PpPR-10 transcript levels increase significantly by treatments with elicitors of Pectobacterium carotovorum subsp. carotovorum, spores of B. cinerea, and the defense hormone salicylic acid. To characterize the role of PpPR-10 in plant defense against pathogens, we conducted overexpression analysis in P. patens and in Arabidopsis thaliana. We demonstrate that constitutive expression of PpPR-10 in moss tissues increased resistance against the oomycete Pythium irregulare. PpPR-10 overexpressing moss plants developed less symptoms and decreased mycelium growth than wild type plants. In addition, PpPR-10 overexpressing plants constitutively produced cell wall depositions in protonemal tissue. Ectopic expression of PpPR-10 in Arabidopsis resulted in increased resistance against P. irregulare as well, evidenced by smaller lesions and less cellular damage compared to wild type plants. These results indicate that PpPR-10 is functionally active in the defense against the pathogen P. irregulare, in both P. patens and Arabidopsis, two evolutionary distant plants. Thus, P. patens can serve as an interesting source of genes to improve resistance against pathogen infection in flowering plants.

  10. Moss Pathogenesis-Related-10 protein enhances resistance to Pythium irregulare in Physcomitrella patens and Arabidopsis thaliana

    Directory of Open Access Journals (Sweden)

    Alexandra eCastro

    2016-04-01

    Full Text Available Plants respond to pathogen infection by activating signaling pathways leading to the accumulation of proteins with diverse roles in defense. Here, we addressed the functional role of PpPR-10, a pathogenesis-related (PR-10 gene, of the moss Physcomitrella patens, in response to biotic stress. PpPR-10 belongs to a multigene family and encodes a protein twice the usual size of PR-10 proteins due to the presence of two Bet v1 domains. Moss PR-10 genes are differentially regulated during development and inoculation with the fungal pathogen Botrytis cinerea. Specifically, PpPR-10 transcript levels increase significantly by treatments with elicitors of Pectobacterium carotovorum subsp. carotovorum, spores of B. cinerea, and the defense hormone salicylic acid. To characterize the role of PpPR-10 in plant defense against pathogens, we conducted overexpression analysis in P. patens and in Arabidopsis thaliana. We demonstrate that constitutive expression of PpPR-10 in moss tissues increased resistance against the oomycete Pythium irregulare. PpPR-10 overexpressing moss plants developed less symptoms and decreased mycelium growth than wild type plants. In addition, PpPR-10 overexpressing plants constitutively produced cell wall depositions in protonemal tissue. Ectopic expression of PpPR-10 in Arabidopsis resulted in increased resistance against P. irregulare as well, evidenced by smaller lesions and less cellular damage compared to wild type plants. These results indicate that PpPR-10 is functionally active in the defense against the pathogen P. irregulare, in both P. patens and Arabidopsis, two evolutionary distant plants. Thus, P. patens can serve as an interesting source of genes to improve resistance against pathogen infection in flowering plants.

  11. Astrocyte Elevated Gene-1 as a Novel Clinicopathological and Prognostic Biomarker for Gastrointestinal Cancers: A Meta-Analysis with 2999 Patients.

    Directory of Open Access Journals (Sweden)

    Yihuan Luo

    Full Text Available There have been numerous articles as to whether the staining index (SI of astrocyte elevated gene-1 (AEG-1 adversely affects clinical progression and prognosis of gastrointestinal cancers. Nevertheless, controversy still exists in terms of correlations between AEG-1 SI and clinicopathological parameters including survival data. Consequently, we conducted a comprehensive meta-analysis to confirm the role of AEG-1 in clinical outcomes of gastrointestinal carcinoma patients.We performed a comprehensive search in PubMed, ISI Web of Science, Cochrane Central Register of Controlled Trials, EMBASE, Science Direct, Wiley Online Library, China National Knowledge Infrastructure (CNKI, WanFang and Chinese VIP databases. STATA 12.0 (STATA Corp., College, TX was used to analyze the data extracted from suitable studies and Newcastle-Ottawa Scale was applied to assess the quality of included articles.The current meta-analysis included 2999 patients and our results suggested that strong associations emerged between AEG-1 SI and histological differentiation (OR = 2.129, 95%CI: 1.377-3.290, P = 0.001, tumor (T classification (OR = 2.272, 95%CI: 1.147-4.502, P = 0.019, lymph node (N classification (OR = 2.696, 95%CI: 2.178-3.337, P<0.001 and metastasis (M classification (OR = 3.731, 95%CI: 2.167-6.426, P<0.001. Furthermore, high AEG-1 SI was significantly associated with poor overall survival (OS (HR = 2.369, 95%CI: 2.005-2.800, P<0.001 and deteriorated disease-free survival (DFS (HR = 1.538, 95%CI: 1.171-2.020, P = 0.002. For disease-specific survival (DSS and relapse-free survival (RFS, no statistically significant results were observed (HR = 1.573, 95%CI: 0.761-3.250, P = 0.222; HR = 1.432, 95%CI: 0.108-19.085, P = 0.786. Subgroup analysis demonstrated that high AEG-1 SI was significantly related to poor prognosis in esophageal squamous cell carcinoma (ESCC (HR = 1.715, 95%CI: 1.211-2.410, P = 0.002, gastric carcinoma (GC (HR = 2.255, 95%CI: 1.547-3.288, P<0

  12. 75 FR 39680 - Houston Pipe Line Company LP, Worsham-Steed Gas Storage, L.P., Energy Transfer Fuel, LP, Mid...

    Science.gov (United States)

    2010-07-12

    ... Storage, L.P., Energy Transfer Fuel, LP, Mid Continent Market Center, L.L.C., Oasis Pipeline, LP (Not... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. PR10-44-000; Docket No. PR10-46-000; Docket No....

  13. A retrospective comparative exploratory study on two Methylentetrahydrofolate Reductase (MTHFR) polymorphisms in esophagogastric cancer: the A1298C MTHFR polymorphism is an independent prognostic factor only in neoadjuvantly treated gastric cancer patients

    International Nuclear Information System (INIS)

    Methylentetrahydrofolate reductase (MTHFR) plays a major role in folate metabolism and consequently could be an important factor for the efficacy of a treatment with 5-fluorouracil. Our aim was to evaluate the prognostic and predictive value of two well characterized constitutional MTHFR gene polymorphisms for primarily resected and neoadjuvantly treated esophagogastric adenocarcinomas. 569 patients from two centers were analyzed (gastric cancer: 218, carcinoma of the esophagogastric junction (AEG II, III): 208 and esophagus (AEG I): 143). 369 patients received neoadjuvant chemotherapy followed by surgery, 200 patients were resected without preoperative treatment. The MTHFR C677T and A1298C polymorphisms were determined in DNA from peripheral blood lymphozytes. Associations with prognosis, response and clinicopathological factors were analyzed retrospectively within a prospective database (chi-square, log-rank, cox regression). Only the MTHFR A1298C polymorphisms had prognostic relevance in neoadjuvantly treated patients but it was not a predictor for response to neoadjuvant chemotherapy. The AC genotype of the MTHFR A1298C polymorphisms was significantly associated with worse outcome (p = 0.02, HR 1.47 (1.06-2.04). If neoadjuvantly treated patients were analyzed based on their tumor localization, the AC genotype of the MTHFR A1298C polymorphisms was a significant negative prognostic factor in patients with gastric cancer according to UICC 6th edition (gastric cancer including AEG type II, III: HR 2.0, 95% CI 1.3-2.0, p = 0.001) and 7th edition (gastric cancer without AEG II, III: HR 2.8, 95% CI 1.5-5.7, p = 0.003), not for AEG I. For both definitions of gastric cancer the AC genotype was confirmed as an independent negative prognostic factor in cox regression analysis. In primarily resected patients neither the MTHFR A1298C nor the MTHFR C677T polymorphisms had prognostic impact. The MTHFR A1298C polymorphisms was an independent prognostic factor in patients with

  14. Investigation of silicon sensors for their use as antiproton annihilation detectors

    Energy Technology Data Exchange (ETDEWEB)

    Pacifico, N., E-mail: nicola.pacifico@cern.ch [University of Bergen, Institute of Physics and Technology, Allégaten 55, 5007 Bergen (Norway); Aghion, S. [Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Istituto Nazionale di Fisica Nucleare, Sez. di Milano, Via Celoria 16, 20133 Milano (Italy); Ahlén, O. [European Organisation for Nuclear Research, Physics Department, 1211 Geneva 23 (Switzerland); Belov, A.S. [Institute for Nuclear Research of the Russian Academy of Sciences, Moscow 117312 (Russian Federation); Bonomi, G. [University of Brescia, Department of Mechanical and Industrial Engineering, Via Branze 38, 25133 Brescia (Italy); Istituto Nazionale di Fisica Nucleare, Sez. di Pavia, Via Agostino Bassi 6, 27100 Pavia (Italy); Bräunig, P. [Kirchhoff Institute for Physics, Im Neuenheimer Feld 227, 69120 Heidelberg (Germany); Bremer, J. [European Organisation for Nuclear Research, Physics Department, 1211 Geneva 23 (Switzerland); Brusa, R.S. [Department of Physics, University of Trento, via Sommarive 14, 38123 Povo, Trento (Italy); INFN-TIFPA, via Sommarive 14, 38123 Povo, Trento (Italy); Burghart, G. [European Organisation for Nuclear Research, Physics Department, 1211 Geneva 23 (Switzerland); Cabaret, L. [Laboratoire Aimé Cotton, CNRS, Université Paris Sud, ENS Cachan, Bâtiment 505, Campus d' Orsay, 91405 Orsay Cedex (France); Caccia, M. [University of Insubria, Dipartimento di Scienza ed Alta Tecnologia, via Valleggio 11, Como (Italy); Canali, C. [University of Zurich, Physics Institute, Winterthurerstrasse 190, 8057 Zurich (Switzerland); Caravita, R. [Istituto Nazionale di Fisica Nucleare, Sez. di Genova, Via Dodecaneso 33, 16146 Genova (Italy); University of Genoa, Department of Physics, Via Dodecaneso 33, 16146 Genova (Italy); Castelli, F. [University of Milano, Department of Physics, Via Celoria 16, 20133 Milano (Italy); and others

    2014-11-21

    We present here a new application of silicon sensors aimed at the direct detection of antinucleons annihilations taking place inside the sensor's volume. Such detectors are interesting particularly for the measurement of antimatter properties and will be used as part of the gravity measurement module in the AEg{sup ¯}IS experiment at the CERN Antiproton Decelerator. One of the goals of the AEg{sup ¯}IS experiment is to measure the gravitational acceleration of antihydrogen with 1% precision. Three different silicon sensor geometries have been tested with an antiproton beam to investigate their properties as annihilation detection devices: strip planar, 3D pixels and monolithic pixel planar. In all cases we were successfully detecting annihilations taking place in the sensor and we were able to make a first characterization of the clusters and tracks.

  15. Thermodynamic clarification of interaction between antiseptic compounds and lipids consisting of stratum corneum

    International Nuclear Information System (INIS)

    The interactions of antiseptic compounds with quaternary ammonium, such as benzalkonium chloride (BC), benzethonium chloride (BZC), dodecyldiaminoethyl-glycine hydrochloride (AEG), and chlorhexidine gluconate (CHG), with components of the stratum corneum were investigated by isothermal titration calorimetry at pH 7.5 and 25 deg. C. The different mechanisms for their permeation to stratum corneum were clarified. Cationic surfactants of BC and BZC bound to cholesterol and cholesterol sulfate with high affinity (105-106 M-1) to extract endogenous cholesterol and its derivatives from the stratum corneum and penetrated via an intercellular route. CHG also bound to cholesterol and accumulated in the stratum corneum without removing endogenous cholesterol. On the other hand, an amphoteric surfactant of AEG seemed to be incorporated into the lipid bilayer and bound to ceramide with its polar end close to the lipid polar heads by hydrophobic interaction

  16. Thermodynamic clarification of interaction between antiseptic compounds and lipids consisting of stratum corneum

    Energy Technology Data Exchange (ETDEWEB)

    Aki, Hatsumi; Kawasaki, Yuhsuke

    2004-06-24

    The interactions of antiseptic compounds with quaternary ammonium, such as benzalkonium chloride (BC), benzethonium chloride (BZC), dodecyldiaminoethyl-glycine hydrochloride (AEG), and chlorhexidine gluconate (CHG), with components of the stratum corneum were investigated by isothermal titration calorimetry at pH 7.5 and 25 deg. C. The different mechanisms for their permeation to stratum corneum were clarified. Cationic surfactants of BC and BZC bound to cholesterol and cholesterol sulfate with high affinity (10{sup 5}-10{sup 6} M{sup -1}) to extract endogenous cholesterol and its derivatives from the stratum corneum and penetrated via an intercellular route. CHG also bound to cholesterol and accumulated in the stratum corneum without removing endogenous cholesterol. On the other hand, an amphoteric surfactant of AEG seemed to be incorporated into the lipid bilayer and bound to ceramide with its polar end close to the lipid polar heads by hydrophobic interaction.

  17. Extended wavelet transformation to digital holographic reconstruction: application to the elliptical, astigmatic Gaussian beams.

    Science.gov (United States)

    Remacha, Clément; Coëtmellec, Sébastien; Brunel, Marc; Lebrun, Denis

    2013-02-01

    Wavelet analysis provides an efficient tool in numerous signal processing problems and has been implemented in optical processing techniques, such as in-line holography. This paper proposes an improvement of this tool for the case of an elliptical, astigmatic Gaussian (AEG) beam. We show that this mathematical operator allows reconstructing an image of a spherical particle without compression of the reconstructed image, which increases the accuracy of the 3D location of particles and of their size measurement. To validate the performance of this operator we have studied the diffraction pattern produced by a particle illuminated by an AEG beam. This study used mutual intensity propagation, and the particle is defined as a chirped Gaussian sum. The proposed technique was applied and the experimental results are presented.

  18. Uudised : "Springjazz" Moskvas. 26. märtsil on Euroraadios Bachi päev. Aarne Saluveer juhib Kooriühingut / Hugo Alter

    Index Scriptorium Estoniae

    Alter, Hugo-Eduard, 1929-2003

    2000-01-01

    20. märtsil Moskvas Eesti saatkonnas toimunud jazzkontserdist "Springjazz". 26. märtsil on Euroopa Ringhäälingute Liitu (EBU) kuuluvatel raadiojaamadel eetris muusikaprogramm "Bach ja tema aeg", millega tähistatakse käesolevat Bachi aastat. 18. märtsil toimunud Eesti Kooriühingu aruande-valimiskoosolekust, kus esitati aruandeid ühingu tegevusest ja valiti juhatuse uueks esimeheks A. Saluveer

  19. Surm kevadises Tallinnas ehk Kunstnike valuline reaktsioon meie ajale / Reet Varblane

    Index Scriptorium Estoniae

    Varblane, Reet, 1952-

    2006-01-01

    Mark Soosaare näitus "Aeg" Tallinna Kunstihoone galeriis kuni 23. IV, Jaan Paavle maalinäitus "1 minut" 3.-15. IV Draakoni galeriis, Sandra Jõgeva ja Kristin Kalamehe "Leave like a Lady" Draakoni galerii keldrisaalis 4.-15. IV, Marianne Männi näitus "Paradiis" Hobusepea galeriis 5.-17. IV. Maailma positiivsemale poolusele osutab 20. IV Tallinna Linnagaleriis avatud Mari ja Kaarel Kurismaa ruumiinstallatsioon "Teine süütus"

  20. Rock'n'roll'i hotellipidaja / Ants Johanson

    Index Scriptorium Estoniae

    Johanson, Ants, 1962-

    2008-01-01

    Ansamblist Rock Hotel (ansambli 30. sünnipäeva tähistamise juubelikontsertdid toimuvad 14. okt. Pärnu Endla teatris, 15. okt. Rakvere Teatris, 16. okt. Viljandi Pärimusmuusika Aidas, 17. okt. Paide kultuurikeskuses, 18. okt. Võru kultuurimajas Kannel, 19. okt. Vanemuise väikeses majas ja 20. okt. Vene Teatris, 9. okt. toimub Tallinnas Viru Keskuse kaupluses Rahva Raamat mälestusteraamatu "Aeg meid muutnud on? - Rock-Hotel 30" esitlus)

  1. EST Table: FY003280 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY003280 bmov10d18 11/11/04 42 %/142 aa ref|XP_001648294.1| Juvenile hormone-induci...ble protein, putative [Aedes aegypti] gb|EAT44634.1| Juvenile hormone-inducible protein, putative [Aedes aeg...A 11/11/04 34 %/139 aa gi|91080979|ref|XP_974925.1| PREDICTED: similar to Juvenile hormone-inducible protein, putative [Tribolium castaneum] FS808716 bmov ...

  2. Kunstikonkurss "Oma silmaga" 2009. aastal / Lili Saksing

    Index Scriptorium Estoniae

    Saksing, Lili

    2009-01-01

    Kehtna Kunstide Kooli korraldatav Raplamaa laste ja noorte kunstikonkurss "Oma silmaga" toimub 2009. a. teemal "Mõttemaja". Ülesandeks on luua tasapinnaline töö, kus kujutatakse Raplamaal asuvat mõttemaja, mille töö autor on ise kavandanud ja sobitanud valitud ümbrusse. Rändnäitus 2008. a. konkursi töödest teemal "Aeg ja mina" on jõudnud Kohila Koolituskeskusesse Tohisoo mõisas

  3. Pesakastide jaoks jääb võimsust ülegi / Rakennusmaailma põhjal Ingrid Maasik

    Index Scriptorium Estoniae

    2011-01-01

    10,8- ja 12voldised akutrell-kruvikeerajad: AEG BS 12 C 2, Biltema 17-275, Black&Decker HPL106K, Bosch GSR 10,8-Li-2, DeWalt DCD710, Einhell BT-CD, Festool CXS, Festool C12Li, Hitachi DS 10 DFL, Makita DF330D, Metabo PowerMaxx 12, Milwaukee C12DD-32C, Nutool 10,8V, Panasonic EY6409

  4. Rahvuse mõju noorte tööturule sisenemise edukusele = The influence of ethnicity on youth labour market entry : III koht magistritööde kategoorias / Kristina Lindemann

    Index Scriptorium Estoniae

    Lindemann, Kristina, 1984-

    2009-01-01

    Magistritöö eesmärgiks oli uurida, kuidas mõjutasid rahvus ja eesti keele oskus noorte tööturule sisenemise edukust ajaperioodil 1991-2006 ning kuidas mõjutasid inimese rahvus, keeleoskus, haridustase, tööturule sisenemise aeg ja elukoht tõenäosust lülituda spetsialistide ja ametnike või sinikraede positsioonile

  5. Tallinna sadamaala linnaruumiline arengukava : ettepanek = Urban Development Plan for the Tallinn Port Area : Proposal

    Index Scriptorium Estoniae

    2006-01-01

    Tellija: Tallinna Linnaplaneerimise Amet. Korraldaja: Eesti Arhitektide Liit. Aeg: 2006. a. veebruar. Osalejad: linnageograaf Hardo Aasmäe, arhitektid Andres Alver, Rein Hansberg, Veljo Kaasik, Ott Kadarik, Erik Konze, Margit Mutso, Tiit Sild, Villem Tomiste, TLPA juht, Tallinna peaarhitekt Igor Volkov, Tallinna Sadama esindaja Hannes Kuhlbach, TLPA projektijuht Jaan Kurm, TLPA üldplaneeringute osakonna juhataja Endrik Mänd, Logi Projekti esindaja Taimi Lokotar, Heldur Meerits

  6. Kui tulusid ei ole võimalik suurendada, tuleb piirata kulutamist / Eugen Veges

    Index Scriptorium Estoniae

    Veges, Eugen, 1955-

    2008-01-01

    Ilmunud ka: Lääne Elu 17. mai lk. 2, Põhjarannik, Severnoje Poberezhje 22. mai lk. 4, Kuulutaja 30. mai lk. 4, Meie Maa 31. mai lk. 2, Vooremaa 3. juuni lk. 2, Järva Teataja 5. juuni lk. 2. Eesti ettevõtjail on ees raske aeg, ettevõtete tulusus langeb. Autori sõnul tuleb riigi tulubaasi suurendamiseks kehtestada uusi makse või suurendada seniseid maksulaekumisi. Haldusreform on möödapääsmatu

  7. Functional and immunohistochemical characterization of CCEae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors Aedes aegypti and Ae. albopictus.

    Science.gov (United States)

    Grigoraki, Linda; Balabanidou, Vassileia; Meristoudis, Christos; Miridakis, Antonis; Ranson, Hilary; Swevers, Luc; Vontas, John

    2016-07-01

    Temephos is a major organophosphate (OP) larvicide that has been used extensively for the control of Aedes albopictus and Aedes aegypti, the major vectors for viral diseases, such as dengue fever, zika and chikungunya. Resistance to temephos has been recently detected and associated with the upregulation of carboxylesterases (CCEs) through gene amplification, in both species. Here, we expressed the CCEae3a genes which showed the most striking up-regulation in resistant Aedes strains, using the baculovirus system. All CCEae3a variants encoded functional enzymes, with high activity and preference for p-nitrophenyl butyrate, a substrate that was shown capable to differentiate temephos resistant from susceptible Aedes larvae. Enzyme kinetic studies showed that CCEae3as from both Ae. aegypti and Ae. albopictus (CCEae3a_aeg and CCEae3a_alb, respectively) strongly interact with temephos oxon and slowly released the OP molecule, indicating a sequestration resistance mechanism. No difference was detected between resistant and susceptible CCEae3a_aeg variants (CCEae3a_aegR and CCEae3a_aegS, respectively), indicating that previously reported polymorphism is unlikely to play a role in temephos resistance. HPLC/MS showed that CCEae3as were able to metabolize temephos oxon to the temephos monoester [(4-hydroxyphenyl) sulfanyl] phenyl O,O-dimethylphosphorothioate. Western blot and immunolocalization studies, based on a specific antibody raised against the CCEae3a_alb showed that the enzyme is expressed at higher levels in resistant insects, primarily in malpighian tubules (MT) and nerve tissues.

  8. Loominguliste ja uuenduslike ideede rakendamisega seotud pinged / Maureen McLaughlin, Mary Ellen Vogt ; tõlkinud Tiina Nurm

    Index Scriptorium Estoniae

    McLaughlin, Maureen

    2000-01-01

    Selleks, et saaks toimuda tulemuslik ja uuenduslik aineõpetus, tuleb teadvustada ja ületada pinged, mis on seotud loovideede rakendamisega. Pingetekitajad, millega õpetajad uuenduslike õpetamis- ja õppimistoimingute rakendamisel võivad kokku puutuda, on järgmised : aeg, ainekavad, tekstid ja muud allikad, oletatav loovuse puudumine, ebakõla uuenduste ja reaalsuse vahel, õpilaste erinevus, tunni organiseerimine ja läbiviimine, hindamine ja hinnangu andmine, õpilaste usaldamine

  9. Julgus palgata puuetega inimesi annaks töökäsi / Agne Narusk

    Index Scriptorium Estoniae

    Narusk, Agne, 1971-

    2005-01-01

    AS-is Standard töötab neli puudega töömeest. Tööandjate keskliidu infojuhi Kadri Seederi hinnangul on mööda saamas aeg, mil tööandjad pelgasid puudega töötajatega kaasnevaid lisakohustusi. Lisa: Tasuta abivahendid. 2002. aasta uuringu kohaselt töötas 27% puudega inimestest. Tugiisiku abi

  10. Rahandusministeerium : Eestit ootab ees galopeeriv hinnatõus / Kai Kalamees

    Index Scriptorium Estoniae

    Kalamees, Kai

    2007-01-01

    Ilmunud ka: Postimees : na russkom jazõke, 23. aug. 2007, lk. 2. Rahandusministeeriumi prognoosi järgi ootab Eestit 8,1%-line majanduskasv, 2008. aastal 7,4%-line hinnatõus. Rahandusminister Ivari Padari ja üle-eelmise rahandusministri parlamendiliikme Taavi Veskimäe arvamusi palgakasvu, inflatsiooni ja lisaeelarve kohta. Vt. samas: Käes on poliitiliste valikute aeg

  11. COMPARATIVE EFFECT OF AEROBICS AND RESISTANCE EXERCISES ON SELECTED PHYSIOLOGICAL VARIABLES AMONG OBESE CHILDREN

    OpenAIRE

    M. Dhanalakshmi; Grace Helina; Senthilkumar

    2015-01-01

    The aim of this study was to find out the comparative effects of aerobics and resistance exercises on selected physiological variables among obese children. To achieve the purpose, 60 Obese children, whose BMI was greater than 30 kg/m2 were randomly selected and assigned into three groups, aerobics exercises group (AEG), resistance training group (RTG) and Control group (CG) consisting of 20 in each. After assessing physiological variables, forced vital capacity and resting heart rate init...

  12. Kus asub Ouagadougou? / Reet Roos

    Index Scriptorium Estoniae

    Roos, Reet, 1973-

    2004-01-01

    Ilmunud ka: Nädaline, 21. okt. 2004, lk. 4; Lõunaleht, 21. okt. 2004, lk. 2; Põhjarannik, 22. okt. 2004, lk. 2; Severnoje Poberezhje, 22. okt. 2004, lk. 2; Meie Maa, 22. okt. 2004, lk. 6; Harjumaa, 26. okt. 2004, lk. 2; Hiiu Leht, 26. okt. 2004, lk. 2; Valgamaalane, 9. nov. 2004, lk. 2; Pärnu Postimees, 7. dets. 2004, lk. 15. Autor, arvustades Eesti haridust, leiab, et tsentraalselt ette antud mudelite alusel koolielu korraldamise aeg on lõppenud

  13. Vabariigi presidendi iseseisvuspäeva kõne / Toomas Hendrik Ilves

    Index Scriptorium Estoniae

    Ilves, Toomas Hendrik, 1953-

    2009-01-01

    Vabariigi Presidendi kõne Eesti Vabariigi 91. aastapäeva pidulikul kontserdil Jõhvi kontserdimajas 24. veebruaril 2009. Arvamust avaldavad Euroopa Parlamendi liige Siiri Oviir, suurärimees Tiit Vähi, Eesti katoliku kiriku piiskop Philippe Jourdan, Tartu Ülikooli rektor Alar Karis, Eesti Kunstiakadeemia rektor Signe Kivi ja helilooja Olav Ehala. Vt. ka lk. 1: Ilves hoiatas populismi eest; juhtkiri lk. 2: Aeg kaitsta vabadust

  14. Analüütikud soovitavad Eesti Energia börsile viia / Piret Reiljan

    Index Scriptorium Estoniae

    Reiljan, Piret, 1983-

    2008-01-01

    Kohalikud analüütikud soovitavad viia Eesti Energia börsile, kuna see elavdaks aktsiaturgu ja tõmbaks ligi välisinvestoreid. Vt. samas: Raivo Vare: raske aeg töötab Eesti Energia börsiletoomise kasuks; Analüüs ootab pääsu valitsuskabinetti; Juhid ei püsi enam Eesti Energias. Diagrammid: Majandusnäitajad; Varade maht

  15. Omad võõraste hulgas / Viktor Matizen ; tõlk. Tamara Korts

    Index Scriptorium Estoniae

    Matizen, Viktor

    2004-01-01

    Moskvas lõppes XXVI rahvusvaheline filmifestival. Peapreemia sai venelase Dmitri Meshijevi "Omad" ("Svoi"), FIPRESCI auhinna Marina Razbezhkina "Lõikuse aeg" ("Vremja zhatvõ"), publiku auhinna Vladimir Mashkovi "Isa" ("Papa!"), vene kriitikute auhinna brasiillase Heitor Dhalia "Nina", konkursi "Perspektiiv" auhinna jaapanlane Hideta Takahata ("Hotell Veenus") ja žürii eriauhinna Hõbedase Püha Jüri eestlaste Jaak Kilmi ja Rene Reinumägi "Sigade revolutsioon"

  16. "Isa kuhu need värvid jäävad ..." : [luuletused] / Jaan Kaplinski

    Index Scriptorium Estoniae

    Kaplinski, Jaan, 1941-

    2002-01-01

    Sisu: "Isa kuhu need värvid jäävad ..." ; Lume ootamine ; "Oh sina paha peavalu ..." ; "Märg on vihmal märg on kastel ..." ; "Peet kisub peeti ..." ; "Küllap väikestel mustadel ..." ; "Mart haigutab ..." ; Kuu ; Teremees Tõnu ; Jalgrataste talveuni ; Kitsu ja kätsu ; Siret siret siterkuuti ; Suve soe silmadesse ; Iirimaal on ilmalapsed ; Miks ; Sügisel ; Täis ; Mis algab ; Aeg ; "Ma koiduajal ärkasin ..."

  17. Facile synthesis of novel 3D nanoflower-like Cu(x)O/multilayer graphene composites for room temperature NO(x) gas sensor application.

    Science.gov (United States)

    Yang, Ying; Tian, Chungui; Wang, Jingchao; Sun, Li; Shi, Keying; Zhou, Wei; Fu, Honggang

    2014-07-01

    3D nanoflower-like CuxO/multilayer graphene composites (CuMGCs) have been successfully synthesized as a new type of room temperature NOx gas sensor. Firstly, the expanded graphite (EG) was activated by KOH and many moderate functional groups were generated; secondly, Cu(CH3COO)2 and CTAB underwent full infusion into the interlayers of activated EG (aEG) by means of a vacuum-assisted technique and then reacted with the functional groups of aEG accompanied by the exfoliation of aEG via reflux. Eventually, the 3D nanoflower consisting of 5-9 nm CuxO nanoparticles homogeneously grow in situ on aEG. The KOH activation of EG plays a key role in the uniform formation of CuMGCs. When being used as gas sensors for detection of NOx, the CuMGCs achieved a higher response at room temperature than that of the corresponding CuxO. In detail, the CuMGCs show a higher NOx gas sensing performance with low detection limit of 97 ppb, high gas response of 95.1% and short response time of 9.6 s to 97.0 ppm NOx at room temperature. Meanwhile, the CuMGC sensor presents a favorable linearity, good selectivity and stability. The enhancement of the sensing response is mainly attributed to the improved conductivity of the CuMGCs. A series of Mott-Schottky and EIS measurements demonstrated that the CuMGCs have much higher donor densities than CuxO and can easily capture and migrate electrons from the conduction band, resulting in the enhancement of electrical conductivity.

  18. Kümme aastat R-klubi / Maria-Maren Sepper

    Index Scriptorium Estoniae

    Sepper, Maria-Maren

    2014-01-01

    R-klubi (aeg-ajalt Tallinnas kogunev klubi, mis pakub tagasisidet eesti keele alases uurimistöös, ennekõike väitekirjauurimuste tegemisel ja artiklite publitseerimisel) 10. aastapäeva puhul peetud seminarist Tallinna Ülikoolis 21.3.2014. R-klubi sai alguse Helle Metslangi juhendatavate TLÜ kraadiõppurite tööde ühisaruteludest

  19. Moesõber, sel nädalal riisu koor Eesti rõivakunstilt! / Tanel Veenre

    Index Scriptorium Estoniae

    Veenre, Tanel, 1977-

    2009-01-01

    3. Tallinna moenädala üritustest. Tallinn Fashion Week'i korraldab Eesti Moedisaini Liit. Näitus "Uus Eesti moejoonistus" Arhitektuuri- ja Disainigaleriis. Filippo Caroti ja Oksana Tanditi moefotonäitus Tallinnas Galeriis M. Meesteehete väljapanek ja Anu Hindi moefotonäitus "Aeg maha 3" Iida galeriis. "Eesti moepiibel 2010" esitlus. Rahvusvaheline seminar Veerenni 24. Ülevaade eesti moekunstnike loomingu esitlustest

  20. A new cyclotron for biomedical research

    International Nuclear Information System (INIS)

    This paper presents the rationale for replacing the old AEG Compact Cyclotron (built in 1969/71) of the Institute for Radiology and Pathophysiology at the German Cancer Research Center by a 30 MeV H-/15 MeV D- cyclotron. A status report is followed by the scientific and technical reasoning as well as budgetary and organizational considerations. In the appendix we tried to explain the function of a cyclotron in a simple and comprehensive manner. (orig.)

  1. Functional and immunohistochemical characterization of CCEae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors Aedes aegypti and Ae. albopictus.

    Science.gov (United States)

    Grigoraki, Linda; Balabanidou, Vassileia; Meristoudis, Christos; Miridakis, Antonis; Ranson, Hilary; Swevers, Luc; Vontas, John

    2016-07-01

    Temephos is a major organophosphate (OP) larvicide that has been used extensively for the control of Aedes albopictus and Aedes aegypti, the major vectors for viral diseases, such as dengue fever, zika and chikungunya. Resistance to temephos has been recently detected and associated with the upregulation of carboxylesterases (CCEs) through gene amplification, in both species. Here, we expressed the CCEae3a genes which showed the most striking up-regulation in resistant Aedes strains, using the baculovirus system. All CCEae3a variants encoded functional enzymes, with high activity and preference for p-nitrophenyl butyrate, a substrate that was shown capable to differentiate temephos resistant from susceptible Aedes larvae. Enzyme kinetic studies showed that CCEae3as from both Ae. aegypti and Ae. albopictus (CCEae3a_aeg and CCEae3a_alb, respectively) strongly interact with temephos oxon and slowly released the OP molecule, indicating a sequestration resistance mechanism. No difference was detected between resistant and susceptible CCEae3a_aeg variants (CCEae3a_aegR and CCEae3a_aegS, respectively), indicating that previously reported polymorphism is unlikely to play a role in temephos resistance. HPLC/MS showed that CCEae3as were able to metabolize temephos oxon to the temephos monoester [(4-hydroxyphenyl) sulfanyl] phenyl O,O-dimethylphosphorothioate. Western blot and immunolocalization studies, based on a specific antibody raised against the CCEae3a_alb showed that the enzyme is expressed at higher levels in resistant insects, primarily in malpighian tubules (MT) and nerve tissues. PMID:27180726

  2. Maailmas pole enam ühtki riski? / Jonathan Compton ; tõlk. Villu Zirnask

    Index Scriptorium Estoniae

    Compton, Jonathan

    2006-01-01

    Autori sõnul on aktsia- ja võlakirjaturud hinnanud sõjaväelist riigipööret Tais ja Põhja-Korea saamist tuumariigiks triviaalseteks ja globaalseid riske vähendavaks. Riski hind näib praegusel ajal olevat pöördvõrdelises seoses olukorraga riigis. Kindel on, et praegu on ohtlik aeg osta arenevate turgude võlakirju, osta Bulgaaria kinnisvara, riskifondide fonde, indeksifonde ja muid investeeringuid

  3. Rossii ne nuzhna milost Zapada / Jonathan Steele

    Index Scriptorium Estoniae

    Steele, Jonathan

    2007-01-01

    Autori hinnangul on Vladimir Putin andnud Venemaale sõltumatuse ja uue rolli rahvusvahelisel areenil. Aeg, mil Läänel oli võimalus avaldada mõju Venemaa sisemisele arengule, on möödas. Tuleb leppida Venemaa sõltumatusega ning mõista, et Venemaasse tuleb suhtuda vähemalt nii, nagu suhtutakse Hiinasse - kui majanduslikku ja strateegilisse giganti

  4. Fukuyama : kõigepealt hea valitsus, siis demokraatia ja ajaloo lõpp / Francis Fukuyama ; interv. Nathan Gardels

    Index Scriptorium Estoniae

    Fukuyama, Francis

    2005-01-01

    Francis Fukuyama sõnul vajab riik oma arenguks esmajoones head valitsemist. Kõige olulisem on, et oleks toimiv riik, mis suudab tagada julgeoleku ja majandusarengu elementaarsed tingimused. Iraagi praeguste probleemide põhjus on selles, et USA ei suutnud ette näha, kuidas riik Saddami kukkudes kokku variseb, kuid tuleb mõista, et aeg-ajalt on USA ja kogu maailma huvides kusagil maailma nurgas riigi ehitamine tõsiselt ette võtta, ütleb autor

  5. The Zebra Battery: a South African contender for electric vehicle application

    Directory of Open Access Journals (Sweden)

    J. Coertzer

    1996-07-01

    Full Text Available The Zebra battery is one of the most promising power sources for electric vehicles which might be on sale before the year 2000. It is a South African development which started at the CSIR and is at present jointly managed by the Anglo American Corpora­tion of S.A. and the German company A.E.G. The chemical reaction converts common salt and nickel to nickel chloride and sodium during the charging phase.

  6. Affective Music Information Retrieval

    OpenAIRE

    Wang, Ju-Chiang; Yang, Yi-Hsuan; Wang, Hsin-Min

    2015-01-01

    Much of the appeal of music lies in its power to convey emotions/moods and to evoke them in listeners. In consequence, the past decade witnessed a growing interest in modeling emotions from musical signals in the music information retrieval (MIR) community. In this article, we present a novel generative approach to music emotion modeling, with a specific focus on the valence-arousal (VA) dimension model of emotion. The presented generative model, called \\emph{acoustic emotion Gaussians} (AEG)...

  7. Lugusid möödunud aegadest / Andrus Kivirähk

    Index Scriptorium Estoniae

    Kivirähk, Andrus, 1970-

    2007-01-01

    Autor toob viimasel ajal loetud kirjandusteoste hulgast välja oma põnevaimad leiud: Mart Kivastik. Külmetava kunstniku portree ; Mart Kivastik. Põrgu wärk ; Mart Kivastik. Kits õnge ja viiuliga ; Galina Vishnevskaja. Aeg ja elu ; Maurice Druon. Neetud kuningad : raudne kuningas ; Maurice Druon. Neetud kuningad : kägistatud kuninganna ; Maurice Druon. Neetud kuningad : kuningavõimu mürgitajad

  8. Poeetilise utoopia meister Aino Bach

    Index Scriptorium Estoniae

    2003-01-01

    28. III-11. V Adamson-Ericu muuseumis graafik Aino Bachi loomingu ülevaatenäitus. Võrdluseks on eksponeeritud Eduard Wiiralti ja Kaarel Liimandi tööd. Näha võib Mark Soosaare filmi A. Bachist "Liblikapüüdja" (1978). Kuraator Anne Lõugas, kujundaja Virge Jõekalda. Kai Tuviku juhendamisel muuseumitunnid "Kuidas joonistada inimest?". 1. IV seminaril "Naiskunstnik ja tema aeg. Aino Bach" esinejad, ettekanded

  9. Teadus- ja erialaraamatukogudel oma päev ja aasta teo auhind / Kristina Rallmann

    Index Scriptorium Estoniae

    Rallmann, Kristina

    2008-01-01

    Esimene teadus- ja erialaraamatukogude päev toimus 22. oktoobril 2008 Tallinna majanduskoolis, teadusraamatukogu aasta teo auhind läks RR-i virtuaaalnäitusele "Meie parlament ja aeg: fakte, sündmusi, dokumente, inimesi" ning erialaraamatukogu aasta teo auhind Eesti Pimedate Raamatukogu töörühmale heliteavikute CD-plaatidele automatiseeritud paljundamise ja posti teel laenutamise süsteemi eest

  10. Aitab hädaldamisest / Robert Antropov

    Index Scriptorium Estoniae

    Antropov, Robert, 1965-

    2006-01-01

    Endine politseijuht on seisukohal, et politseinike ja päästjate nappuses ei saa süüdistada ainult väikseid palku, küsimus on valikutes: kuidas ja millesse ressursse paigutada. Kalle Laanet ja Raivo Aeg vastavad küsimusele, mis on nende haldusalas konkreetselt kavas, et politsei saaks täita oma tegevusprioriteeti 100% ja tagada sisejulgeoleku. Vt. ka Robert Antropovi kommentaari 7. aug. Eesti Päevaleht lk. 2

  11. Iirimaale : [luuletused] / Percy Bysshe Shelley ; tlk. Minni Nurme, Ants, Oras

    Index Scriptorium Estoniae

    Shelley, Percy Bysshe

    2006-01-01

    Sisu: Iirimaale ; Armastuse roos ; Harrietile ; Muutlikkus ; Vangist vabanenud sõbrale ; Minevik ; Byronile ; Kaduvik ; Laul : "Maa magas, külm kui surm..." ; Hümn vaimsele ilule ; Laul : "Too aeg on läind, mu armsam, läind..." ; Ozymandias ; Sonett ; India serenaad ; Leonardo da Vinci Medusa Firenze kunstikogus ; Armu filosoofia ; Ood läänetuulele ; Mary Shelleyle ; Laul briti meestele ; Uus rahvushümn ; Inglismaa 1819. a. ; Ära madu ärata ; Head ööd! ; Pilv ; Apolloni hümn ; Paani hümn ; Lõoke ; Vabadus ; Suvi ja talv ; Maailmarändurid ; Küsimus ; Aeg, mis hajus ; Ööle ; -le : "Üht sõna on labastand suud..." ; -le : "Kui kirg kord mööduks, aga jääks..." ; Õhtu Ponte al Marel, Pisas ; Mälestus ; Muusika ; Aeg ; Põgenejad ; Ma olla kuningas ei tahaks ; "Homne päev, kus oled praegu..." ; "Laps rahus kord on emaihus..." ; Üksik lind ; "Kui lamp pihuks langend..." ; Epitaaf ; Kutse Janeþile ; Tähed vilkusid valgust ; Leinalaul ; Pühendus. Eluloolisi andmeid autori kohta lk. 389

  12. Application of Alkylethoxyglucoside in Pesticide Microemulsion%醇醚糖苷在农药微乳液中的应用

    Institute of Scientific and Technical Information of China (English)

    朱福送; 杨秀全; 白亮; 樊文俊; 张军

    2011-01-01

    [目的]醇醚糖苷(AEG)是一种新型绿色功能性表面活性剂,在农药制剂中尚少有应用.研制含醇醚糖苷的啶虫脒微乳液,并将其与常规的乳油制剂做润湿力、动态接触角和药液沉积量对比试验,考察醇醚糖苷在农药制剂中应用的实际效果及可行性.[结果]通过溶剂、表面活性剂复配的筛选及拟三元相图的绘制,确定了以醇醚糖苷与十二烷基苯磺酸钠(LAS)复配作乳化剂,制备了3%和5%啶虫脒微乳液.与常规的乳油制剂相比,用醇醚糖苷制备的微乳液表面活性剂用量低,润湿性和药液沉积量优于同类乳油产品.[结论]醇醚糖苷性能优越,可作为农药乳化剂和增效剂使用.%[Aims] Alkylethoxyglucoside (AEG) is a new biodegradable functional surfactants, and still rarely be applied in pesticide formulations. Here, the formulation of acetamiprid microemulsion containing AEG was prepared and compared to conventional EC formulation on wetting properity, dynamic contact angle and pesticide solution retention to study the actual effect and feasibility evaluation of application of alkylethoxyglucoside in pesticide formulations. [Results] By optimizing the solvents, surfactants systems and drawing pseudoternary phase diagram, the formulation of acetamiprid 3 and 5% microemulsion was prepared using the compounded system of AEG and LAS as emulsifier. As compared to the common EC formulation, the acetamiprid microemusion prepared by AEG consumed less amount of surfactants, possessed better wetting properity and larger pesticide solution retention. [Conclusions] Alkylethoxyglucoside has superior performance, and can be used as a new pesticide emulsifier and synergist.

  13. Assessment of non-invasive time and frequency atrial fibrillation organization markers with unipolar atrial electrograms

    International Nuclear Information System (INIS)

    The standard electrocardiogram (ECG) is the most common non-invasive way to study atrial fibrillation (AF). In this respect, previous works have shown that the surface lead V1 reflects mainly the dominant atrial frequency (DAF) of the right atrium (RA), which has been widely used to study AF. In a similar way, AF organization and fibrillatory (f) wave amplitude are two recently proposed non-invasive AF markers. These markers need to be validated with invasive recordings in order to assess their capability to reliably reflect the internal fibrillatory activity dynamics. In this work, these two non-invasive metrics have been compared with similar measures recorded from two unipolar atrial electrograms (AEGs). For both ECG and AEG signals, AF organization has been computed by applying a nonlinear regularity index, such as sample entropy (SampEn), to the atrial activity (AA) and to its fundamental waveform, defined as the main atrial wave (MAW). The surface and epicardial f wave amplitude has been estimated through their mean power. Results obtained for 38 patients showed statistically significant correlations between the values measured from surface and invasive recordings, thus corroborating the usefulness of the aforesaid markers in the non-invasive study of AF. Precisely, for AF organization computed from the MAW, the correlation coefficients between surface and both AEGs were R = 0.926 (p < 0.001) and R = 0.932 (p < 0.001). For f wave amplitude, slightly lower significant relationships were noticed, the correlation coefficients being R = 0.765 (p < 0.001) and R = 0.842 (p < 0.001). These outcomes together with interesting linear relationships found among the parameters suggest that AF regularity estimated via SampEn and f wave amplitude can non-invasively characterize the epicardial activity related to AF

  14. Perioperative nutrition support of adenocarcinoma of the esophagogastric junction%食管胃结合部腺癌围手术期营养支持

    Institute of Scientific and Technical Information of China (English)

    王强; 赵毅

    2012-01-01

    食管胃结合部是指食管末段和近侧胃相连接的部位.食管胃结合部腺癌(AEG)作为一类不同于食管癌和胃癌的独立疾病的临床观点已经被多数学者所接受.然而AEG的围手术期营养支持特点及选择还需进一步研究、探讨.AEG病人常有不同程度的营养不良和免疫功能低下,围手术期合理应用肠内、肠外营养支持对AEG术后病人减少并发症,促进恢复有重要的作用.%The primary treatment of adenocarcinoma of theesophagogastric junction (AEG) is surgery, but more than 50% of curatively resected patients undergo recurrence or cancer-related death during postoperative 5 years. It implies multidisciplinary treatment of radio- and chemotherapy is still necessary. Recently, neoadjuvant therapy has been paid more attention to, and neoadjuvant radiotherapy is currently considered able to improve 5-year overall survival. Moreover, European Organization for Research and Treatment of Cancer has made a consensus guideline of neoadjuvant radiotherapy for AEG, which is useful in clinical practice. Neoadjuvant chemotherapy is meaningful to increase R0 resection rate, but should combine adjuvant chemotherapy to improve overall survival. Concept on adjuvant chemotherapy is according to gastric cancer, and NCCN guideline recommends classic ECF or modified ECF regimens. Results of monotherapy SI trial also showed improved overall survival. The evidence of intra-or postoperative radiotherapy is poor and can not improve long term survival. Therefore, preoperative concurrent chemoradiotherapy plus postoperative chemotherapy is currently preferable multidisciplinary treatment model.

  15. 乌饭树树叶水提取物改善糖尿病小鼠血糖和血脂水平的研究%Effect of Vaccinium bracteatum Thunb.leaves aqueous extract on blood glucose and plasma lipid levels in streptozotocin-induced diabetic mice

    Institute of Scientific and Technical Information of China (English)

    王立; 张雪彤; 章海燕; 程素娇; 钱海峰; 张晖; 张英

    2012-01-01

    The hypoglycemic effects of Vaccinium bracteatum Thunb.leaves(VBTL) aqueous extract in streptozotocin-induced diabetic mice was studied.The aqueous extract of VBTL showed obviously hypoglycemic and hypolipidemic effects,but the effects were weaker than those of dimethylbiguanide.Compared with diabetic mice in model control group,body weights of aqueous extract group(AEG,diabetic mice treated with VBTL aqueous extract) were partly recovered.The BG levels of AEG were reduced 14.2%,and body weights were increased 61.0%.AEG had significantly lower(p0.05) TC or TG levels and similar HDL-cholesterol or LDL-cholesterol levels.In conclusion,research clearly indicated that the aqueous extract of VBTL possess a potential hypoglycemic effect in streptozotocin-induced diabetic mice.%以链脲佐菌素造模糖尿病小鼠为研究对象,对乌饭树树叶水提取物的降血糖和血脂作用进行了研究,并与二甲双胍的效果进行了比较。结果表明,虽然效果没有二甲双胍明显,但水提物对糖尿病小鼠的血糖和血脂有明显的改善作用。相对模型对照组,灌胃四周水提物后糖尿病小鼠的体重增加了61.0%,血糖降低了14.2%,血脂水平也得到了明显的改善。

  16. Balti põgenike problemaatika USA poliitikas 1945-1952 / Kaja Kumer-Haukanõmm

    Index Scriptorium Estoniae

    Kumer-Haukanõmm, Kaja

    2007-01-01

    Teise maailmasõja järel lähtus enamik riike pagulaste kohta käivate otsuste langetamisel USA tegevusest. Sõjapõgenike küsimusega tegelenud organisatsioonidest. Perioodi jooksul toimunud muutustest põgenikelaagrites viibinute suhtes. Keeruline aeg Balti riikide pagulaste jaoks oli 1946. aasta ja 1947. aasta esimene pool. Tõenäoliselt ei abistanud USA balti põgenikke ainult humanistlikel kaalutlustel, vaid neid kasutati külma sõja algul Nõukogude Liiduga suheldes sageli manipuleerimisvahendina

  17. "Möödapääsmatust hädast sunnituna otsustanud ja nõustunud meid Poola Kuninglikule Majesteedile truualamlikuks teha" : Liivimaaseisuste alistumine Poola võimule 1561. aasta sügisel / Enn Tarvel

    Index Scriptorium Estoniae

    Tarvel, Enn, 1932-

    2011-01-01

    Poola-Leedu huvist ja tegevusest Liivimaa suhtes enne 1561. aastat, mil Rootsi aktiivne sekkumine Põhja-Eestis muutis otsustavamaks ka Poola-Leedu sekkumise Liivimaal. 1561. aasta sügisel pidas ordumeister Kettler poolakatega salaläbirääkimisi ja otsustas alistuda. 28. novembril 1561 andsid ordumeister, Riia peapiiskop, orduaadli ning Võnnu ja Volmari linna esindajad truudusvande Sigismund II Augustile. Tegelik Poola aeg algas Eestis 1582 ja kestis 1625. aastani. Dokument: Poola kuninga Sigismund Augusti ja ordumeister Gotthardi vahel sõlmitud alistumisleping. Vilniuses 28. novembril 1561("Pacta subjectionis")

  18. Suvi tulvil festivale

    Index Scriptorium Estoniae

    2005-01-01

    Suvel toimuvate musikafestivalide tutvustus: Eduard Tubin ja tema aeg (muusikafestival), Pärnu rahvusvaheline ooperimuusika festival 2005, Juu Jääb 2005 (retromuusikafestival), Suure-Jaani muusikapäevad 2005, Mravinski Festival 2005, Seitsme linna muusika 2005, Hiiumaa kammermuusikapäevad 2005, Saaremaa suvemuusikafestival 2005, David Oistrahhi festival 2005, Klaaspärlimäng (festival) 2005, Rapla kirikumuusika XIII festival 2005, Kuressaare ooperipäevad 2005, Pärnu ooperipäevad 2005, Tallinna XIX orelifestival, Haapsalu XII viiulimängud 2005, Viljandi vanamuusikafestival 2005, Kuressaare kammermuusikapäevad 2005, Haapsalu vanamuusikafestival 2005, Võru Muusikapäevad 2005

  19. Eesti Teadusfondi preemia Astrid Haljasele ja Tõnu Laasile

    Index Scriptorium Estoniae

    2000-01-01

    Eesti Teadusfondi täppisteaduste ekspertkomisjoni poolt korraldatud magistrantide ja doktorantide teadustööde konkursil määrati preemia 4000 krooni loodusteaduste osakonna teadurile Astrid Haljasele artikli Mankin, R., Ainsaar, A., Haljas, A. Trichotomous-noise-induced phase transitions for the stochastic Hongler model. Proc. Estonian Acad. Sci. Phys. Math., 2000, 49, 1, 28-39 eest. Loodusteaduste osakonna assistendile, TPÜ doktorandile Tõnu Laasile määrati preemia 7000 krooni artiklite sarja Lainete levik kõveras aeg-ruumis eest : [täistekst

  20. Kinoelamus muutub kolmemõõtmeliseks / Jaanus Noormets

    Index Scriptorium Estoniae

    Noormets, Jaanus

    2008-01-01

    Sellest nädalast näidatakse Tallinnas Coca Cola Plazas tänu uuele erilisele tehnoloogiale ka kolmemõõtmelisi filme (3D-filme): detsembris 2008 - "Reis maakera südamesse" ("Journey to the Centre of the Earth"); jaanuaris 2009 - "Välk" ("Bolt"), märts 2009: "My Bloody Valentine"; aprillis 2009 - "Koletised tulnukate vastu" ("Monsters vs. Aliens") ; kevadel 2009 - "Coraline" ; juulis 2009 - "Jääaeg 3". Sellest reedest alates näidatakse kobarkinos 3D-animafilmi "Kärbsed Kuu peale" ja muusikafilmi "U2 3D"

  1. Retraso del crecimiento extrauterino en recién nacidos prematuros y su relación con factores de riesgo cardiovascular a los 2 años de edad corregida: papel de la leptina, adiponectina, perfil lipídico y adiposidad

    OpenAIRE

    Salvador Iglesias, Miriam

    2016-01-01

    La desnutrición extrauterina es un hecho frecuente entre la población de recién nacidos prematuros y no está claro si ésta acarrea un mayor riesgo de complicaciones metabólicas futuras. OBJETIVOS: Evaluar si existen diferencias en las concentraciones de adiponectina y leptina a los 2 años de edad corregida (EC) entre recién nacidos prematuros (RNPT) de 32 semanas o menos que nacen con un peso adecuado a la edad gestacional (AEG) y sufren una restricción del crecimiento extrauterino (RCEU) a l...

  2. RHEOLOGICAL BEHAVIOUR OF PSYLLIUM GUM FRACTIONS

    Directory of Open Access Journals (Sweden)

    Mohammad Hojjatoleslamyi

    2013-10-01

    Full Text Available Psyllium (Plantago psyllium is a native plant that grows widely in India, Iran and Pinjab. Studies showed psyllium gum has good rheological properties for using in wide range of food products. In this study, different fractions of psyllium gum extracted by water and alkali treatment. Rheological properties of these fractions determined by Brookfield rheometer (RV DVIII. Obtained data fitted in three temperatures 30, 60 and 80°C by Herschel-bulkly rheological model. Results showed that fractions have different behaviour during heating treatment. The most difference observed in AEG0.5 fraction.

  3. Kas tegeleda uue töölepinguseadusega või mitte? / Väino Linde

    Index Scriptorium Estoniae

    Linde, Väino, 1959-

    2008-01-01

    Töölepingu seaduse eelnõust, kehtiva töölepingu puudustest ning kompromissi vajalikkusest. Ilmunud ka Sakala 23. jaan. 2008, lk. 2, pealkiri kujul: aeg otsida kompromisse ; Vali Uudised 23. jaan. 2008, lk. 2 ; Põhjarannik 22. jaan. 2008, lk. 4 ; Meie Maa 23. jaan. 2008, lk. 2 ; Hiiu Leht 25. jaan. 2008, lk. 2 ; Vooremaa 24. jaan. 2008, lk. 2 ; Võrumaa Teataja 24. jaan. 2008, lk. 2 ; Pärnu Postimees 25. jaan. 2008, lk. 15 ; Lääne Elu 26. jaan. 2008, lk. 2, Virumaa Teataja 29. jaan. 2008, lk. 11

  4. Arhitektuuriaasta 2004 = The year in architecture 2004 / Epp Lankots

    Index Scriptorium Estoniae

    Lankots, Epp, 1976-

    2005-01-01

    Arhitektuuriauhindade nominentidest ja premeeritutest. Eesti Kultuurkapitali aastapreemia sai Toomas Rank Rägavere mõisa renoveerimise eest. Arhitektuuri sihtkapitali renoveerimispreemia - Mari Kurismaa, Eesti Draamateatri interjööride renoveerimine; objektipreemia - Martin Aunin, Ülemiste hotell; interjööripreemia - Jan Joonas Graps, Ken-Kristjan Ruut, "Jungenti" büroo; arhitektuurse tegevuse preemia - Kristjan Holm, Liina Jänes, Agu Külm, Jukka-Pekka Jalovaara, Pille Epner, Madis Jürgen, Tõnu Kaalep, Ingmar Muusikus, Eesti ekspositsioon "Aeg-maha-arhitektuur" Veneetsia arhitektuuribiennaalil

  5. 油脂基表面活性剂在矿物浮选中的应用%Oil based surfactants in the application of mineral flotation

    Institute of Scientific and Technical Information of China (English)

    任小荣; 牛金平; 韩亚明

    2009-01-01

    综述了以天然油脂为原料生产的几种表面活性剂,如脂肪酸及其衍生物、脂肪醇醚羧酸盐(AEG)、脂肪醇醚磷酸酯、脂肪胺及其衍生物在矿物浮选中的应用.认为矿物浮选是表面活性剂的重要应用领域之一,且该行业对表面活性剂的需求正在迅速增加.

  6. Tallinn ja Moskva austagu teineteist / Terry Davis

    Index Scriptorium Estoniae

    Davis, Terry

    2007-01-01

    Ilmunud ka: Molodjozh Estonii, 8. mai 2007, lk. 4; Postimees : na russkom jazõke, 9. mai 2007, lk. 9. Euroopa Nõukogu peasekretär mõistab hukka vandalismi ja rüüstamise Tallinna ja Moskva tänavatel Teise maailmasõja mälestusmärgi teisaldamise pärast ning ütleb, et on aeg käituda väärikalt nii teineteise kui mineviku suhtes, kuigi see võib olla vastuoluline

  7. The new Class 611 diesel railcar with active tilting technology; Der neue Dieseltriebzug Baureihe 611 mit aktiver Neigetechnik

    Energy Technology Data Exchange (ETDEWEB)

    Sauer, B.; Schoenberg, S; Waetzold, S. [ABB Daimler-Benz Transportation GmbH, Henningsdorf (Germany)

    1996-06-01

    Starting with the successful Class 610, AEG Schienenfahrzeuge GmbH has been developing and building a new vehicle class with the AEG neicontrol-E active tilt technology - the Class 611. Deutsche Bahn AG has ordered 50 railcars, of which the first go into service in spring 1996. They will operate on lines that are mostly non-electrified and have frequent curves and they will run at up to 160 km/h. The new design incorporates much expertise derived from the building and operation of the VT 610, the result being a vehicle that meets the requirements of passenger- and environment-friendliness and is also easy to maintain and is economical. The electrical tilting technology has been jointly developed by AEG Schienenfahrzeuge and the Daimler-Benz subsidiary ESW. A space-saving arrangement underfloor was chosen, and it is also low in weight and largely maintenance-free. The components used have proved themselves over many years in military applications under extreme climatic conditions. (orig.) [Deutsch] Aufbauend auf der bewaehrten Baureihe 610 entwickelt und baut die ABB Daimler-Benz Transportation (Deutschland) GmbH eine neue Generation von Fahrzeugen mit der aktiven elektrischen Neigetechnik AEG neicontrol-E - die Baureihe 611. Adtranz baut unter Beteiligung der DWA 50 Triebzuege fuer die Deutsche Bahn AG, von denen die ersten im Fruehjahr 1996 in Betrieb gesetzt werden. Die Einsatzgebiete werden bogenreiche, ueberwiegend nicht elektrifizierte Strecken sein, die das neue Fahrzeug mit 160 km/h befaehrt. Viele Erfahrungen aus Bau und Betrieb des VT 610 flossen in die Konzeption ein, so dass ein den Anforderungen von Fahrgast- und Umweltfreundlichkeit, Wartung und Wirtschaftlichkeit entsprechendes Fahrzeug bereitsteht. Die elektrische Neigetechnik ist eine gemeinsame Entwicklung der ABB Daimler-Benz Transportation (Deutschland) GmbH und der Daimler Benz Tochter EXTEL SYSTEMS WEDEL (ESW). Sie ist platzsparend unterflur angebracht und zeichnet sich durch geringes Gewicht

  8. Comparison of advanced high power underground cable designs

    International Nuclear Information System (INIS)

    In this paper, advanced high power underground cable designs are compared in the light of available literature, of reports and information supplied by participating industries (AEG, BICC, CGE, Pirelli, Siemens), spontaneous contributions by EdF, France, BBC and Felten and Guilleaume Kabelwerke A.G., Germany, and Hitachi, Furukawa, Fujikura and Sumitomo, Japan, and earlier studies carried out at German public research centres. The study covers cables with forced cooling by oil or water, SF6-cables, polyethylene cables, cryoresistive and superconducting cables. (orig.)

  9. Nõukogude postmodernism = Soviet postmodernism / Ants Juske

    Index Scriptorium Estoniae

    Juske, Ants, 1956-

    2010-01-01

    Analüüsitakse, kuidas ja milliseid teid pidi igasugused "-ismid" jõudsid läbi raudse eesriide Ida-Euroopa maadesse. Esimestena haakusid postmodernismiga avangardistlikud arhitektid, seejärel toimusid nihked ka kujutavas kunstis, kunstiteaduses ja -kriitikas. Nõukogude aeg jättis jälje meie kunstile, kuid see "nõukogude" on rohkem iroonilises võtmes mõistetud, sisaldades alltekste - kas tegemist on šedöövriga või kunstnik lihtsalt lollitab - ja "nõukogude postmodernism" on seetõttu läänele raskesti mõistetav

  10. Tähtede ja laulukooridega jõulutuuritama / Mari Hiiemäe

    Index Scriptorium Estoniae

    Hiiemäe, Mari

    2008-01-01

    Jõulukontsertidest: Annely Peebo ja Revalia Kammermeeskoori "Jõulureis läbi ajastute", Dave Bentoni ja segakoori Sõpruskond "Häid jõule!", Maarja-Liis Ilusa ja Tallinna Poistekoori "Püha öö", Rolf Roosalu ja Nele-Liis Vaiksoo "Imede öö", Anna-Liisa Suppi ja Sofia Rubina "Südamele südamest", Liisi Koiksoni ja Marko Matvere "Jõululood", Ivo Linna ja Suisapäisa "Kaunimad jõululaulud", "Jõulutuur" koos Tallinna Kammerorkestriga, Birgit Õigemeele ja Uku Suviste "Ilus aeg" ETV lastekooriga

  11. Trumbid on Keskerakonna käes / Andrus Villem

    Index Scriptorium Estoniae

    Villem, Andrus

    2004-01-01

    Ilmunud ka: Elva Postipoiss, 4. dets. 2004, lk. 4; Lääne Elu, 7. dets. 2004, lk. 2; Järva Teataja, 7. dets. 2004, lk. 2; Harjumaa, 7. dets. 2004, lk. 2; Vooremaa, 7. dets. 2004, lk. 2; Koit, 7. dets. 2004, lk. 6; Pärnu Postimees, 8. dets. 2004, lk. 15; Sakala, 8. dets. 2004, lk. 2; Hiiu Leht, 10. dets. 2004, lk. 2; Kesknädal, 15. dets. 2004, lk. 11. Autor leiab, et praeguse koosseisuga koalitsiooni aeg on läbi ning hetkel on Keskerakond parimas olukorras

  12. Ajaloo läbistav valgus / Andri Ksenofontov

    Index Scriptorium Estoniae

    Ksenofontov, Andri, 1962-

    2010-01-01

    Vano Allsalu näitus "Süütuse aeg" Tallinnas Hobusepea galeriis 22.09.-4.10. 2010. Franyó Aatothi (István Ferenc Tóth) näitus Ungari Instituudis 15. oktoobrini 2010. Monika Žaltauskaitė-Grašienė, Severija Inčirauskaitė-Kriaunevičienė ja Monika Järg'i näitus "Side minevikust / Connected with the past" Tallinnas galeriis Hop 10.-28. septembrini 2010

  13. 1st February 2011-CERN Cultural Board for Engaging with the Arts, visiting CMS experimental area and LHC Tunnel at Point 5

    CERN Multimedia

    Michael Hoch

    2011-01-01

    Photo 1-4: Visit to CMS Control Room with G. Tonelli,CMS Collaboration Spokesperson Photo 5-9,16-20:CMS experimental area Photo 10-15:LHC Tunnel at Point 5 Photo 21:F. Madlener,Director of IRCAM Paris+S. Dorny,Director-General Lyon Opera House+C. Bollman,Art by Genève+M. Doser,AEgIS Collaboration Spokesperson,Former Physics Department Deputy Head+A. Koek,International Arts Development+G. Tonelli+M. Monje Cano,Arts Development Assistant (part-time work experience)+B. Ruf,Director of Kunsthalle Zürich

  14. Näitused

    Index Scriptorium Estoniae

    1998-01-01

    Draakoni galerii - Nele Zirnite näitus "Aja märk", Vaal-galerii - Mall Nukke näitus "Mängude aeg - Play Time", Linnagalerii - TPÜ üliõpilaste ühisnäitus "Paldiski lood", La Galerie Passage - näitus "Üdised algused" - 5 eesti, 1 saksa kunstnik, Tammsaare muuseum - Austraalias elanud eesti kunstniku Karl Manbergi maalide ja pisiplastika näitus, Kinomaja - Valeri Sakovi maalide näitus, Kullo galerii - lasteaialaste ja pensionäride tööde näitus

  15. Naiskunstnike invasioon : juhus või paratamatus? / Reet Varblane

    Index Scriptorium Estoniae

    Varblane, Reet, 1952-

    2006-01-01

    Christel Alliku lillemaalid Draakoni galeriis kuni 4. III; Nelly Drelli maalinäitus "Taassünd" Viviann Napa galeriis kuni 15. III; Silva Eheri maalinäitus "Midagi ei toimu" G-galeriis kuni 4. III; Jarõna Ilo joonistused "(Aasta)aeg" Hausis kuni 3. III; Kristiina Kaasiku maalinäitus "Ad astra" Ühispanga galeriis kuni 3. III; Krista Möldri "Proloog maamärkidele" Deco galeriis kuni 4. III; Eveli Variku "Sünnimärgid" Tallinna raekoja fotokeldris "Lee" kuni 6. III; Kadri Alesmaa ja Jaak Visnapi "Disainitud kunst. Kõik müügiks" Tallinna Linnagaleriis kuni 5. III

  16. Short Outlines of Books by Estonian authors / Rutt Hinrikus

    Index Scriptorium Estoniae

    Hinrikus, Rutt, 1946-

    2007-01-01

    Tutvustus: Miljutina, Tamara. Inimesed minu elus. Tallinn : Varrak, 2007 ; Soosaar, Enn. Isa ja aeg. Tallinn : Kultuurileht, 2007. (Loomingu Raamatukogu ; 5) ; Paju, Imbi. Tõrjutud mälestused. Tallinn : Eesti Entsüklopeediakirjastus, 2007 ; Kaplinski, Jaan. Seesama jõgi. Tallinn : Vagabund, 2007 ; Laanem, Tiina. Väikesed vanamehed. Tallinn : Eesti Päevalehe Kirjastus, 2007 ; Vaarandi, Debora. Aastad ja päevad. Tallinn : Tänapäev, 2006 ; Berg, Maimu. Unustatud inimesed. Tallinn : Tuum, 2007 ; Heinsaar, Mehis. Rändaja õnn. Tallinn : Verb, 2007

  17. Meie kobar PÖFFi peamises põõsas / Tarmo Teder

    Index Scriptorium Estoniae

    Teder, Tarmo, 1958-

    2007-01-01

    Pimedate Ööde Filmifestivalist, Eesti filmide võistlusprogrammist, mille auhinna rahastajaks on Scottish Leader Eesti. Konkurssprogrammis on 12 täispikka filmi: "Sügisball" (rezh. Veiko Õunpuu), "Nuga" (rezh. Marko Raat), "Dirigendi kõla" (rezh. Helle Karis, Peter Murdmaa), "Vastutuulesaal" (rezh. Priit Valkna), "Klass" (rezh. Ilmar Raag), "Kuhu põgenevad hinged" (rezh. Rainer Sarnet), "Tervitusi Nõukogude Eestist!" (rezh. Urmas Eero Liiv), "Jonathan Austraaliast" (rezh. Sulev Keedus), "Georg" (rezh. Peeter Simm), "Debora Vaarandi aeg" (rezh. Katrin Laur), "Jan Uuspõld läheb Tartusse" (rezh. Andres Maimik, Rain Tolk), "Põdra kuningriik" (rezh. Rein Maran)

  18. 17. X avati XIV rahvusvaheline Tallinna graafikatriennaal, seekord ülddeviisi "Poliitiline/Poeetiline" all / Eve Kask

    Index Scriptorium Estoniae

    Kask, Eve

    2007-01-01

    Tallinna XIV graafikatriennaalist (Anders Kreugeri koostatud näitus "1987. Baltimaade graafika ja plakatid nõukogude aja viimasest kümnendist" ja põhinäitus "Poliitiline/Poeetiline" Kumu Kunstimuuseumis, Anders Härmi kuraatorinäitus "Poliitiline/Poeetiline" Tallinna Kunstihoones ja selle galeriis, Egle Kuckaite isikunäitus Tallinna Linnagaleriis, triennaali paneel Kadrioru Kunstimuuseumis). Triennaali peaauhinna sai Oscar Munos. Triennaaliga samaaegselt Tallinnas toimuvast rahvusvahelisest graafikakonverentsist "Impact 5" teemal "Lõigatud aeg", mille raames on avatud graafikanäitused

  19. Electron ray facilities for the pasteurization of sewage sludges

    International Nuclear Information System (INIS)

    Growing industrialization and the simultaneous increase in population density demand broad preventive measures in the area of waste water and sewage sludges. Electron irradiation is becoming an important tool for disinfection in this field. The AEG-Telefunken sludge pasteurization process works in continuous operation with homogenized sludge at electron energies between 1,0 to 1,5 MeV and a radiation dosage of 4 kJ/kg. The system offers the capabilities for an effective and costadvantageous disinfection of waste sludges of differing consistencies and origins and their harmless reuse as fertilizer in agriculture. (orig.)

  20. "Ostanovimsja na mig v tishine..." : [luuletused] / Ene Mihkelson ; lühibiograafia: T. L. [Toomas Liiv

    Index Scriptorium Estoniae

    Mihkelson, Ene, 1944-

    1999-01-01

    Sisu: "Ostanovimsja na mig v tishine..." / tlk. V. Fadin ; Rekviem / tlk. Marju Lauristin ; "Dom eto tolko godõ..." ; "Dvoje bezumtsev mezh nimi treti..." ; "Eva v Tapa zhivjot / tlk. Boris Tuch ; "Ljagushka prõgnula skvoz lezvije kossõ..." ; "Vse stantsii peremeshtshalis..." ; "Dõb iz trub stekajet..." ; "Vremja neuklonno i surovo..." / tlk. M. Korol ; Pjat tshassov kogda snega net / tlk. Jelena Salkova. Orig.: Reekviem ; "Konn hüppas vikatist läbi..." ; "Kõik jaamad segunesid Valisime keeli..." ; "Suits sajab korstnaist maha..." ; "Aeg on karm ja ühepoolne Meid..."; Kell viis kui pole lund

  1. Mass Wasting Following the 2002 Missionary Ridge Fire near Durango, Colorado, a Field Trip Guidebook

    Science.gov (United States)

    Bigio, Erica R.; Blair, Robert W.; Burke, Michael; Cannon, Susan H.; deWolfe, Victor G.; Ey, John; Gartner, Joseph E.; Gillam, Mary L.; Knowlton, N.D.; Santi, Paul M.; Schulz, William H.; Coe, Jeffrey A.

    2007-01-01

    This field trip guide focuses on mass wasting following the 2002 Missionary Ridge fire near Durango, Colorado. We prepared this guide to accompany a May 4, 2006, field trip during the second Roy J. Shlemon Specialty Conference, which was held in Durango, Colorado, May 3-5. The conference, entitled Mass Wasting in Disturbed Watersheds, was sponsored by the Association of Environmental & Engineering Geologists (AEG) and the AEG Foundation. The objective of this Shlemon Conference was to bring together practitioners and researchers to define the current state of practice and identify unresolved problems with regard to the prediction and mitigation of mass wasting in disturbed watersheds. The one-day field trip begins and ends in Durango. Many of the field trip stops are at debris-flow fans around the periphery of the burn area, but one stop examines landslide activity in the burn area that initiated during spring 2005 snowmelt within a dormant, deep-seated landslide, as well as an erosion/debris-flow mitigation effort in a drainage basin above Lemon Reservoir. Also provided are descriptions of the Missionary Ridge fire, the geologic and climatic setting of the field-trip area, and the general effects of wildfire on watersheds.

  2. Detection of Cyanotoxins, β-N-methylamino-L-alanine and Microcystins, from a Lake Surrounded by Cases of Amyotrophic Lateral Sclerosis

    Directory of Open Access Journals (Sweden)

    Sandra Anne Banack

    2015-01-01

    Full Text Available A cluster of amyotrophic lateral sclerosis (ALS has been previously described to border Lake Mascoma in Enfield, NH, with an incidence of ALS approximating 25 times expected. We hypothesize a possible association with cyanobacterial blooms that can produce β-N-methylamino-L-alanine (BMAA, a neurotoxic amino acid implicated as a possible cause of ALS/PDC in Guam. Muscle, liver, and brain tissue samples from a Lake Mascoma carp, as well as filtered aerosol samples, were analyzed for microcystins (MC, free and protein-bound BMAA, and the BMAA isomers 2,4-diaminobutyric acid (DAB and N-(2-aminoethylglycine (AEG. In carp brain, BMAA and DAB concentrations were 0.043 μg/g ± 0.02 SD and 0.01 μg/g ± 0.002 SD respectively. In carp liver and muscle, the BMAA concentrations were 1.28 μg/g and 1.27 μg/g respectively, and DAB was not detected. BMAA was detected in the air filters, as were the isomers DAB and AEG. These results demonstrate that a putative cause for ALS, BMAA, exists in an environment that has a documented cluster of ALS. Although cause and effect have not been demonstrated, our observations and measurements strengthen the association.

  3. Tuning a Schottky barrier of epitaxial graphene/4H-SiC (0001) by hydrogen intercalation

    Energy Technology Data Exchange (ETDEWEB)

    Dharmaraj, P.; Justin Jesuraj, P.; Jeganathan, K., E-mail: kjeganathan@yahoo.com [Centre for Nanoscience and Nanotechnology, School of Physics, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu (India)

    2016-02-01

    We report the electron transport properties of epitaxial graphene (EG) grown on 4H-SiC (0001) by low energy electron-beam irradiation. As-grown EG (AEG) on SiC interface exhibits rectifying current-voltage characteristics with a low Schottky barrier (SB) of 0.55 ± 0.05 eV and high reverse current leakage. The SB of AEG/SiC junction is extremely impeded by the Fermi level pinning (FLP) above the Dirac point due to charged states at the interface. Nevertheless, a gentle hydrogen intercalation at 900 °C enables the alleviation of both FLP and carrier scattering owing to the saturation of dangling bonds as evidenced by the enhancement of SB (0.75 ± 0.05 eV) and high electron mobility well excess of 6000 cm{sup 2} V{sup −1} s{sup −1}.

  4. Metadherin/astrocyte elevated gene-1-based DNA vaccine suppresses progression and metastasis in prostate cancer of mice%异黏蛋白/星形细胞上调基因-1靶点的基因疫苗诱导免疫抑制小鼠前列腺癌生长及转移

    Institute of Scientific and Technical Information of China (English)

    张春; 李惠长; 钱本江; 刘昌明; 李国敏; 薛清平

    2013-01-01

    Objective To study the metadherin (MTDH)/astrocyte elevated gene-1 (AEG-1) as targets for gene vaccine inducing immunosuppression mice prostate cancer growth and metastasis of action research.Methods Male C57BL/6 mice (6-8 weekes old) were randomly divided into three groups (n =20),respectively,to phosphate buffered saline (PBS),Pub,Pub-MTDH/AEG-1 attenuated salmonella (1 × 10s cfu) of lavage servo immune; Immune once a week,a total of immune three times.Last one week after the immunization,all in three groups of mice prostate in situ (right and left dorsal lobe) vaccinated 10s RM-1 cell to build orthotopic transplantation tumor model of prostate,induced spontaneous pelvic cavity and retroperitoneal lymph node metastasis; Record each group mice with tumor size,pelvic cavity and retroperitoneal lymph node metastasis number [hematoxylin and eosin (HE) staining shall prevail],CD8 in through immunohistochemical method to detect in situ tumor,the expression of vascular endothelial growth factor (VEGF) and lymphatic vessel endothelial hyaluronic acid receptor-1 (LYVE-1) case,tumor cell apoptosis with TdT-mediated dUTP nick end labeling (TUNEL) method.Results pUb-MTDH/AEG-1 DNA vaccine group compared to the pUb,the PBS group could significantly stimulate CD8 + T cells and cytotoxic T lymphocyte (CTL) response; Effectively inhibit the growth of prostate cancer in situ in mice,MTDH group tumor volume (0.248 ± 0.102) compared with the Pub group (1.475 ± 0.314) (P < 0.01),pelvic cavity and retroperitoneal lymph node metastasis 77.78%,35.7% (P < 0.05) ; Significantly enhanced the expression of CD8 + in the in situ tumor molecular:MTDH group (3.56 ± 0.85),Pub group (5.12 ± 0.90) (P < 0.05),and promote tumor cell apoptosis:MTDH group (39.60 ± 3.28) %,Pub group (16.18 ±2.52)% (P<0.01).Conclusion MTDH/targets AEG-1 gene vaccine in mice prevention immune models,can effectively induce the body specificity immune response,enhance cellular immunity and humoral

  5. The hundredth Gentner Doctoral Student has started at CERN

    CERN Document Server

    CERN Bulletin

    2016-01-01

    Almost ten years after the start of the programme in 2007, the hundredth Gentner Doctoral Student started his PhD at CERN.   The hundredth Gentner Doctoral Student, Christian Zimmer, in front of the AEgIS detector in the AD hall, where he will spend a significant portion of the next 3 years. In 2007, the German Gentner Doctoral Student Programme was established at CERN, named in honour of the celebrated nuclear physicist Wolfgang Gentner, President of the CERN Council from 1972-74. On 1 July 2016, the 100th Gentner Doctoral Student, Christian Zimmer, started his PhD at CERN, where he will work on setting up the sympathetic laser cooling of antiprotons at the AEgIS experiment. CERN’s Doctoral Student Programme has been running for many years, with 200 students currently enrolled. The Gentner programme is fully integrated into the general CERN Doctoral Student Programme, but is entirely funded by the German Federal Ministry of Education and Research (BMBF). The programme sponsors 30 to 40...

  6. Selecting and optimizing of the heavy metal inactivator in sewage sludge composting

    Institute of Scientific and Technical Information of China (English)

    HUANG Yaxi; LI Ji; LI Guoxue; SUN Xue; HUANG Yang; XU Lianshuang

    2007-01-01

    In sewage sludge composting, adding ash from stove (FA20) inactivated active Cu effectively and the results were significantly better than control. For Zn, Mn, Pb and Cd, satisfying results was achieved by adding ash from stove and phosphorite powder (FA20, PR10), phosphorite powder and lime (PR15, CL1O), ash from stove and phosphorite powder (FA10, PR10), and ash from stove and peat (FA20, PT10). Based on their effects of inactivating available forms of heavy metals, ash from stove, phosphorite powder and peat were supposed to be effective inactivators with optimum percentages of 20%, 15% and 10% (FA20, PR15, PT10).

  7. ISOLATION AND IDENTIFICATION OF MYCOPARASITIC ISOLATES OF TRICHODERMA ASPERELLUM WITH POTENTIAL FOR SUPRESSION OF BLACK POD DISEASE OF CACAO IN CAMEROON

    Science.gov (United States)

    Alternative measures are needed to control Phytophthora megakarya, the main causal agent of black pod disease in Africa. Precolonized plate and detached cacao pod assays were used to screen fungal isolates for mycoparasitism on P. megakarya. Only Trichoderma asperellum isolates 659-7, PR10, PR11, a...

  8. 食管胃结合部腺癌流行趋势、病因及防治%Adenocarcinoma of the esophagogastric junction:epidemic trends, causes, prevention and therapy

    Institute of Scientific and Technical Information of China (English)

    陈志峰

    2012-01-01

    Population epidemiology analysis of esophageal cancer for the high risk region in China finds that: incidence of esophageal squamous cell carcinoma has decreased and that of adenocarcinoma of the esophagogastric junction(AEG) has increased significantly. European and American scholars believe that obesity and stomach-esophageal reflux disease are independent risk factors of the two types of cancer. In the high risk region of esophageal cancer in China, the heritability is (29.67±4.32)% and the segregation ratio is 0.181, 95% CI (0.15-0.21). Esophageal cancer belongs to multi-gene genetic disease. According to the evolution of the pathological mechanism of gastric cardia adenocarcinoma and the WHO's clinical treatment principles, cardiac severe atypicalhyperplasia were mainly endoscopic resection in the high riskregion in China. Paying attention to the intervention ofatrophic gastritis or active gastritis may be the realisticmeasures to reduce intestinal metaplasia and the incidence ofAEG.%国内食管癌高发区人群流行病学分析发现:食管鳞癌发病率下降;食管胃结合部腺癌(AEG)发病率显著上升.欧美学者认为,肥胖和胃食管反流性疾病是这两类肿瘤的独立危险因素.我国食管癌高发区的遗传度为(29.67±4.32)%,分离比0.181,95%CI:0.15~0.21,属于多基因遗传病.根据AEG的病理演变机理和WHO的临床处理原则,目前食管癌高发区对贲门重度不典型增生者主要采用内镜下切除;而重视萎缩性或活动性胃炎的干预治疗,对降低肠化,减少AEG的发病也可能是比较现实的措施之一.

  9. A moiré deflectometer for antimatter

    CERN Document Server

    Aghion, S; Amsler, C; Ariga, A; Ariga, T; Belov, A S; Berggren, K; Bonomi, G; Braunig, P; Bremer, J; Brusa, R S; Cabaret, L; Canali, C; Caravita, R; Castelli, F; Cerchiari, G; Cialdi, S; Comparat, D; Consolati, G; Derking, H; Di Domizio, S; Di Noto, L; Doser, M; Dudarev, A; Ereditato, A; Ferragut, R; Fontana, A; Genova, P; Giammarchi, M; Gligorova, A; Gninenko, S N; Haider, S; Huse, T; Jordan, E; Jørgensen, L V; Kaltenbacher, T; Kawada, J; Kellerbauer, A; Kimura, M; Knecht, A; Krasnicky, D; Lagomarsino, V; Lehner, S; Magnani, A; Malbrunot, C; Mariazzi, S; Matveev, V A; Moia, F; Nebbia, G; Nedelec, P; Oberthaler, M K; Pacifico, N; Petracek, V; Pistillo, C; Prelz, F; Prevedelli, M; Regenfus, C; Riccardi, C; Røhne, O; Rotondi, A; Sandaker, H; Scampoli, P; Storey, J; Subieta Vasquez, M A; Spacek, M; Testera, G; Vaccarone, R; Widmann, E; Zavatarelli, S; Zmeskal, J

    2014-01-01

    The precise measurement of forces is one way to obtain deep insight into the fundamental interactions present in nature. In the context of neutral antimatter, the gravitational inter- action is of high interest, potentially revealing new forces that violate the weak equivalence principle. Here we report on a successful extension of a tool from atom optics—the moire ́ deflectometer—for a measurement of the acceleration of slow antiprotons. The setup con- sists of two identical transmission gratings and a spatially resolving emulsion detector for antiproton annihilations. Absolute referencing of the observed antimatter pattern with a photon pattern experiencing no deflection allows the direct inference of forces present. The concept is also straightforwardly applicable to antihydrogen measurements as pursued by the AEgIS collaboration. The combination of these very different techniques from high energy and atomic physics opens a very promising route to the direct detection of the gravitational acceleratio...

  10. Secondary emission monitor for keV ion and antiproton beams

    CERN Document Server

    Sosa, Alejandro; Bravin, Enrico; Harasimowciz, Janusz; Welsch, C P

    2013-01-01

    Beam profile monitoring of low intensity keV ion and antiproton beams remains a challenging task. A Sec- ondary electron Emission Monitor (SEM) has been de- signed to measure profiles of beams with intensities below 107 and energies as low as 20 keV. The monitor is based on a two stage microchannel plate (MCP) and a phosphor screen facing a CCD camera. Its modular design allows two different operational setups. In this contribution we present the design of a prototype and discuss results from measurements with antiprotons at the AEgIS experiment at CERN. This is then used for a characterization of the monitor with regard to its possible future use at different facilities.

  11. Paide - paekivilinn

    Index Scriptorium Estoniae

    2004-01-01

    Juuni: Tallinna Kunstihoone kunstnike näitus, Markus Kasemaa ja Paide huvikeskuse 4.-5. klasside õpilaste maalinäitus ning Mariann Rea fotonäitus Vallitornis. Juuli: 7. Paide paepäevad 1.-10. VII (10 nime loetletud) 8. VII paekonverents "Paide paesel pinnal" Järvamaa muuseumis. Esinevad Eino Tomberg, Helle Perens jt. 10. VII 8. rahvusvaheline tegevuskunsti festival "Aeg. Ruum. Liikumine" Keskväljakul, skulptorid esitlevad valminud skulptuure Vallimäel. 1.-10. VII Eesti Kujurite Ühenduse fotonäitus "Skulptuur fotol" (lk. 19) ja rühmituse 312A näitus Vallitornis. 30. VI-08. VIII portselaninäitus "Kruus kui meene" Järvamaa muuseumis. August: G-galerii kunstnike näitus ja Salme Allikseina maalinäitus Vallitornis, Arvo Auna maalinäitus Järvamaa muuseumis

  12. Short outlines of books by Estonian authors / Janika Kronberg, Rutt Hinrikus

    Index Scriptorium Estoniae

    Kronberg, Janika, 1963-

    2004-01-01

    Tutvustus: Talvik, Heiti. Legendaarne. Tartu : Ilmamaa, 2004 ; Pilter, Lauri. Lohejas pilv : romaan lühijuttudes. Tallinn : Tuum, 2004 ; Raud, Rein. Hector ja Bernard. Tallinn : Tuum, 2004 ; Ruben, Aarne. Elajas trepi eelastmel. Tallinn : Tuum, 2004 ; fs. 2004. Tallinn : Tuum, 2004 ; Normet, Dagmar. ...ainult võti taskus. Tallinn : Varrak, 2004 ; Künnap, Asko. Ja sisalikud vastasid (kolmes kirjas). Tallinn : Näo Kirik, 2003 ; Kall, Toomas. Armas aeg. Tallinn : Tänapäev, 2004 ; Õunapuu, Ervin. Eesti gootika II. Tallinn : Umara, 2004 ; Soomets, Triin. Leping number 2 : luuletusi 2000-2003. Tallinn : Tuum, 2004 ; Tuulik, Jüri. Linnusita : [jutustused, lühijutud, näidendid ja vested]. Tallinn : Maalehe Raamat, 2004 ; Raudam, Toomas. Teie. Tallinn : Eesti Keele Sihtasutus, 2003 ; Härm, Viiu. Keegi teeline : luuletused. Tallinn : Varrak, 2004 ; Härm, Viiu. Õhuaken : romaan. Tallinn : Varrak, 2004

  13. "Kes kaunis on, peab andma vastse võsu..." : [sonetid] / William Shakespeare ; tlk. Harald Rajamets, Ants Oras

    Index Scriptorium Estoniae

    Shakespeare, William, 1564-1616

    2006-01-01

    Sisu: "Kes kaunis on, peab andma vastse võsu..." ; "Sa peeglist näed: need palgejooned nüüd..." ; "Seesama aeg, mis kunstipeenelt voolib..." ; "Miks muusika teeb nukraks sind, kes ise..." ; "Ei, armastust ei tunne sinu rind..." ; "Nii ruttu, kui sa hääbud, uueks sirgud..." ; "Kui kuulen, kuidas aega mõõdab kell..." ; "Kuid miks sa ise tõhusamal moel..." ; "Kas suvepäeva sarnaseks sind kiita?..." ; "Aeg, õgard, küüned lõvil tömbiks kääna... ; "Ei veena peegel mind, et olen vana..." ; "Just nagu näitleja, kel kinni keel..." ; "Mu silm kui taidur võttis pilti teha..." ; "Mu armu isand, kelle truu vasall..." ; "Ma reisist väsinuna heidan sängi..." ; "Kui õnnest hüljatuna nutan sala..." ; "Kui vaikse leebe mõtte kohtu ette..." ; "Ma olen näinud küll, kuis tõusev päike..." ; "Ma tunnistan, et meid on siiski kaks..." ; "Nii nagu isa, vanadusest nõrka..." ; "Kui sulen laud, mu silmad hästi näevad..." ; "Nii ränk on reisida mul tüütul teel..." ; "Mis on su olemus, mis tuum su, miks..." ; "Ei kuningate uhked marmorsambad..." ; "Arm, uuenda end, nii et öelda sust..." ; "Kui uut ei olegi, vaid kõik on sama..." ; "Nii nagu lained ulgumerelt randa..." ; "Neiks päeviks, kui kord aeg mu armsal palge..." ; "Et näen, kuis aja käsi kõike lõhub..." ; "Kui pronksi, kivi, maad ja merd võib neelda..." ; "Mu tusk on ränk, nüüd surma kutsuks küll..." ; "Kui suren, ära leina mind sa kaua..." ; "Sa minus seda aastaaega näed..." ; "Mul sinust kirjutades arg on meel..." ; "Oo hüvasti! Liig kallis mul su hind..." ; "Siis vihka mind, kui pead, ja kohe praegu..." ; "Mõnd uhkeks teeb ta kunst, mõnd sünnipära..." ; "Sust eemal olin kevadel, mu arm..." ; "Kui möödaläinud aja kroonikais..." ; "Mis on veel ajul paberile kanda..." ; "Jah, tõsi, olen käinus siin ja seal..." ; "Ma uskuda ei saa, et murda suudab..." ; "On parem olla halb kui halvaks peetud..." ; "Ei, aeg, sa ära hoople, et mind muudad..." ; "Mu muusika, kui muusikat sa teed

  14. "Kas võib tõesti tulla Hommik?..." : [luuletused] / Emily Dickinson ; tõlk. Doris Kareva, Jüri Talvet

    Index Scriptorium Estoniae

    Dickinson, Emily

    2008-01-01

    Sisu: "Kas võib tõesti tulla Hommik?..." ; "Ta suri - nii ta surigi..." ; "On kuskil surematud Mäed..." ; "Likööri rüüpan võrratut..." ; "See linnuke on Lootus..." ; "Olen Eikeegi. Ja sina?..." ; "Ma tean, on piirat Aeg..." ; "Ma räägin kuidas tõusis Päev..." ; "On Kuudel lõpp - on Aastail - sõlm..." ; "Suur Hullus pühim mõistus on..." ; "Kui saabuksid sa Sügisel..." ; "Hing valib Seltsi ainuomase..." ; "Ma surin Ilu eest - just siis..." ; "Et mina ei saand peatuda..." ; "Surm, see on kahekõne vaid..." ; "Suur Lootus langes..." ; "Üht kaotust igavesti kandsin..." ; "Need kes on hauas viibinud kaua..." ; "See lõplikkusetunne..." ; "Jah - nende tabamatus..." ; "Lõid Maja ümber laksu vood..." ; "Ei pälvi kuigivõrd...". Elu- ja loomeloolisi andmeid autori kohta lk. 507-508

  15. Fluorous Peptide Nucleic Acids: PNA Analogues with Fluorine in Backbone (γ-CF2-apg-PNA) Enhance Cellular Uptake.

    Science.gov (United States)

    Ellipilli, Satheesh; Ganesh, Krishna N

    2015-09-18

    Fluorous PNA analogues possessing fluorine as inherent part of aminopropylglycine (apg) backbone (γ-CF2-apg PNA) have been synthesized and evaluated for biophysical and cell penetrating properties. These form duplexes of higher thermal stability with cRNA than cDNA, although destabilized compared to duplexes of standard aeg-PNA. Cellular uptake of the fluorinated γ-CF2-apg PNAs in NIH 3T3 and HeLa cells was 2-3-fold higher compared to that of nonfluorinated apg PNA, with NIH 3T3 cells showing better permeability compared to HeLa cells. The backbone fluorinated PNAs, which are first in this class, when combined with other chemical modifications may have potential for future PNA-based antisense agents. PMID:26322827

  16. "Ma vannun tema niuete kui paleuse nimel ..." : [luuletused] / tlk. Andres Ehin, tlk. Ly Seppel

    Index Scriptorium Estoniae

    2004-01-01

    Sisu: "Ma vannun tema niuete kui paleuse nimel ..." ; "Ta on nagu kuu, kelle kuma kõik kummuli kukutab ..." ; "Kuu südaöösel ennustab me jäävat lahkujääma ..." ; "Ei hinda naisterahvas kasinuse mulli ..." ; "Ta nõtket kaela kutsun ligemale ..." ; "Meid ühte heitma tõmbab armuköis ..." ; "Meil kahel pole mõtet lahku minna ..." ; "Ei kuuga saa võrrelda sind, sest et kuu läheb looja ..." ; "Allah ise su ilude hoob on ja kang ..." ; "Kui meesterahvale läeb korda meesterahvas ..." ; "Ja kuis ka kiremöll tal südames ei hulla ..." ; "Teie jaoks ei ole minu võlurohke kallike ..." ; "Oli see vast poisike, nägu ilu täis ..." ; "Aeg tagasi kalli las toob, et mind suisa salvata ...". Tundmatute autorite kirjutatud luuletused jutukogust "1001 ööd" (9. - 10. saj)

  17. Two pioneering artists visit CERN

    CERN Multimedia

    CERN Bulletin

    2015-01-01

    On Monday, 19 January, CERN physicists welcomed musician Tim Blake - progressive rock keyboard and theremin player - and architectural lighting designer Patrice Warrener - inventor of the Chromolithe Polychromatic Illumination system, used in Lyon’s “Fête des Lumières”. Together, they make up the musical duo "Crystal Machine".   The artists visit the Antiproton Decelerator. (Image: Django Manglunki.)   Their visit began with an introduction to CERN by their friend Django Manglunki, project leader for the ion injector chain, and an improvised discussion on the LHC extraction system with Roger Barlow, kicker magnet controls expert and progressive rock fan. This was followed by a quick trip to the CCC, the server room and the SPS RF amplifiers in BA3. Next on the itinerary was a tour of the AD and anti-hydrogen experiments led by Michael Doser, AEgIS Spokesperson. A leisurely lunch followed, in the company ...

  18. Steps towards the design of a cryogenic manipulator for an ultra-thin membrane in a 1T magnetic field

    CERN Document Server

    Vasev, Andrej

    2015-01-01

    The main purpose of my CERN's project is design, analysis and research on all conditions under which one cryogenic mechanism would produce extremely low temperatures needed for cooling down a silicon target in a closed magnetic system. Main source of the research is the AEgIS (Antihydrogen Experiment: Gravity, Interferometry, Spectroscopy) experiment of CERN, which aims to carry out the first direct measurement of a gravitational effect on an antimatter system. To reach this goal, a silicon target is bombarded by positrons in order to create positronium which is used to form antihydrogen atoms on which the experiment is going to be done. That would not be possible without the presence of a respective cooling system that secures constant extremely low temperatures in the silicon area. In the further development of the cryogenic system’s functionality, a special attention is given to its construction, used materials and interaction between each other.

  19. President kutsub koostööle / Arnold Rüütel

    Index Scriptorium Estoniae

    Rüütel, Arnold, 1928-

    2002-01-01

    Ilmunud ka: Postimees (2002) 25. veebr., lk. 11, pealk.: Vajame uut ühiskondlikku lepet; SL Õhtuleht (2002) 25. veebr., lk. 10, pealk.: Aeg rahvuslikuks leppeks, vt. samas karikatuur; Virumaa Teataja (2002) 26. veebr., lk. 7, pealk.: Eestis tuleb taastada sotsiaalne tasakaal; Maaleht (2002) 28. veebr., lk. 8, pealk.: Uhkus ajab upakile; Põhjarannik (2002) 26. veebr., lk. 5, pealk.: President Arnold Rüütli kõne Eesti Vabariigi 84. aastapäeval; Severnoje Poberezhje (2002) 26. veebr., lk. 5; Meie Maa (2002) 26. veebr., lk. 2, pealk.: Vajame põlvkondlikku järjepidevust ja uut ühiskondlikku lepet. President Arnold Rüütli kõne Eesti Vabariigi 84. aastapäeval Estonia teatris 24. veebruaril 2002. Lühendatult

  20. 27 Estonian Music Festivals 2002

    Index Scriptorium Estoniae

    2003-01-01

    2002. aastal Eestis toimunud muusikafestivalidest: opeNBaroque 2002 (muusikafestival),Akadeemilised Trompetipäevad, Klavessiinimuusika Päevad, Eesti muusika päevad 2002,Mravinski Festival, Rudolf Tobiase muusikafestival, Eduard Tubin ja tema aeg (muusikafestival), Suure-Jaani muusikapäevad 2002,Regiöö (muusikafestival), David Oistrahhi festival, Haapsalu vanamuusikafestival 2002, Viljandi vanamuusikafestival 2002, Hiiumaa kammermuusikapäevad 2002, Klaaspärlimäng (festival),Mustjala Festival, Haapsalu viiulimuusika Päevad 2002, Kuressaare ooperipäevad 2002, Leigo järvemuusika 2002, Tallinna Orelifestival, Rapla kirikumuusika X festival 2002, Kuressaare kammermuusikapäevad 2002, Mart Saare muusikafestival, Credo 2002, Tartu vanamuusika IX festival, Trialogos 2002 (kultuurifestival), Fiesta de la guitarra (kitarrimuusika festival), Klaver 2002

  1. Beam test results of 3D silicon pixel sensors for future upgrades

    Science.gov (United States)

    Nellist, C.; Gligorova, A.; Huse, T.; Pacifico, N.; Sandaker, H.

    2013-12-01

    3D silicon has undergone an intensive beam test programme which has resulted in the successful qualification for the ATLAS Insertable B-Layer (IBL) upgrade project to be installed in 2013-2014. This paper presents selected results from this study with a focus on the final IBL test beam of 2012 where IBL prototype sensors were investigated. 3D devices were studied with 4 GeV positrons at DESY and 120 GeV pions at the SPS at CERN. Measurements include tracking efficiency, charge sharing, time over threshold and cluster size distributions as a function of incident angle for IBL 3D design sensors. Studies of 3D silicon sensors in an anti-proton beam test for the AEgIS experiment are also presented.

  2. Schöneweide - neu kollagiert. Ein künstlerisches Forschungsprojekt

    Directory of Open Access Journals (Sweden)

    Albert Markert

    2015-04-01

    Full Text Available Der Berliner Stadtteil Schöneweide – einst AEG-Areal und Wiege der Elektrifizierung Berlins - war bis zur Wende einer der größten Industriestandorte Europas. Nach der Wende schlossen nahezu alle Industriebetriebe. Seitdem stehen die meisten Fabrikhallen leer. Seit einiger Zeit wandelt sich Oberschöneweide: Die Hochschule für Technik und Wirtschaft hat seit einigen Jahren hier ihren Hauptstandort; eine international expandierende Kunstgießerei hat eine neue Produktionsstätte eröffnet; der kanadische Rocksänger und Fotograf Bryan Adams plant, ein Kulturzentrum zum internationalen Austausch in einer der ehemaligen Produktionshallen zu etablieren. Seit einigen Jahren ziehen zahlreiche Designer_innen, Künstler_innen und Student_innen nach Schöneweide und prägen zunehmend das Erscheinungsbild des Stadtteils (...

  3. Milne, a routine for the numerical solution of Milne's problem

    Science.gov (United States)

    Rawat, Ajay; Mohankumar, N.

    2010-11-01

    The routine Milne provides accurate numerical values for the classical Milne's problem of neutron transport for the planar one speed and isotropic scattering case. The solution is based on the Case eigen-function formalism. The relevant X functions are evaluated accurately by the Double Exponential quadrature. The calculated quantities are the extrapolation distance and the scalar and the angular fluxes. Also, the H function needed in astrophysical calculations is evaluated as a byproduct. Program summaryProgram title: Milne Catalogue identifier: AEGS_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEGS_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: Standard CPC licence, http://cpc.cs.qub.ac.uk/licence/licence.html No. of lines in distributed program, including test data, etc.: 701 No. of bytes in distributed program, including test data, etc.: 6845 Distribution format: tar.gz Programming language: Fortran 77 Computer: PC under Linux or Windows Operating system: Ubuntu 8.04 (Kernel version 2.6.24-16-generic), Windows-XP Classification: 4.11, 21.1, 21.2 Nature of problem: The X functions are integral expressions. The convergence of these regular and Cauchy Principal Value integrals are impaired by the singularities of the integrand in the complex plane. The DE quadrature scheme tackles these singularities in a robust manner compared to the standard Gauss quadrature. Running time: The test included in the distribution takes a few seconds to run.

  4. Properties of multilayer coatings produced by coaxial laser cladding

    Science.gov (United States)

    Petrovskiy, V. N.; Bykovskiy, D. P.; Dzhumaev, P. S.; Polskiy, V. I.; Prokopova, N. M.; Chirikov, S. N.

    2016-09-01

    This article contains results of the study of multilayer coatings produced by laser cladding on the substrate steel 34HMA using iron based powder PR-10R6M5 as the filler material. The coatings were produced with consistent application of the tracks with fixed overlapping. The dependencies between the characteristics of tracks and the technological mode of deposition were revealed. Properties of coatings were determined for various overlapping of tracks and directions of the cladding layers.

  5. Fractal dimension crossovers in turbulent passive scalar signals

    NARCIS (Netherlands)

    Grossmann, Siegfried; Lohse, Detlef

    1994-01-01

    The fractal dimension δg(1) of turbulent passive scalar signals is calculated from the fluid dynamical equation. δg(1) depends on the scale. For small Prandtl (or Schmidt) number Pr < 10-2 one gets two ranges, δg(1) = 1 for small-scale r and δg(1) = 5/3 for large r, both as expected. But for large P

  6. Birch pollen allergy: molecular characterization and hypoallergenic products

    OpenAIRE

    Schenk, M.F.

    2008-01-01

    Allergic diseases, such as hay fever and food allergy, affect a substantial part of the population in westernized countries. Pollen of the European white birch (Betula pendula) is a considerable cause of hay fever (seasonal allergic rhinitis) in northern and central Europe. The major birch pollen allergen is Bet ν 1, which is the conventional allergen name for the birch pollen proteins of a large group of proteins otherwise known as PR-10 proteins. Individuals that suffer from birch pollen al...

  7. The herbicide flumioxazin stimulates pathogenesis-related gene expression and enzyme activities in Vitis vinifera.

    Science.gov (United States)

    Castro, Antonio Jesús; Saladin, Gäelle; Bézier, Annie; Mazeyrat-Gourbeyre, Florence; Baillieul, Fabienne; Clément, Christophe

    2008-11-01

    In this work, the capacity of the soil-applied herbicide flumioxazin (fmx) to trigger defence mechanisms was assessed using 6-week-old in vitro grown Vitis vinifera L. plantlets. Time-course studies demonstrated that the herbicide induced the expression of basic beta-1,3-glucanase (Vvglu), basic chitinase (Vvchit1b) and PR10 (VvPR10.3) genes encoding three pathogenesis-related (PR) proteins involved in grapevine defence against pathogens. Thus, all transcripts accumulated in grapevine tissues to reach maximum values after 24-72 h of herbicide exposure, except for VvPR10.3 gene expression, which was induced in roots and stems but not in leaves. Induction of PR genes was observed to a greater extent in roots and leaves, and its intensity diminished in the stems although still remained noteworthy. The activities of beta-1,3-glucanase and chitinase enzymes significantly increased in the whole plant after herbicide exposure and were still stimulated 21 days after the beginning of treatments. Similarly, the most remarkable effect occurred in roots. However, all enzyme activities tested were stimulated in the upper aerial tissues as well, indicating that fmx or a derived product acts systemically, likely via root uptake.

  8. Antioxidant capacity and fatty acid profile of Centaurea kotschyi (Boiss. & Heldr. Hayek var. persica (Boiss. Wagenitz from Turkey

    Directory of Open Access Journals (Sweden)

    Aktumsek, Abdurrahman

    2011-03-01

    Full Text Available The antioxidant capacity of the methanolic extract and the fatty acid composition of C. kotschyi var. persica were investigated. Six different chemical methods were used to determine the antioxidant capacity. The fatty acid composition was analyzed using gas chromatography. The IC50 value of the extract was determined as 37.09 μg/ml (in the DPPH assay. In the β-carotene/linoleic acid system, the extract exhibited 65.22% inhibition against linoleic acid oxidation. The amount of total phenolic content and total antioxidant capacity were detected as 36.52 mg gallic acid equivalent (GAE/g and 74.93 mg ascorbic acid equivalent (AE/g, respectively. The major fatty acid in the composition of C. kotschyi var. persica was found to be C 18:3 ω3 (α-linolenic acid by GC analysis. The results presented here indicate that C. kotschyi var. persica possess strong antioxidant properties. Therefore, the species can be used as a natural additive in food, cosmetic and pharmaceutical industries.La capacidad antioxidante de extractos metanólicos y composición de ácidos grasos de C. kotschyi var. pérsica fueron investigados. Seis métodos químicos diferentes fueron realizados para la determinación de la capacidad antioxidante. La composición de ácidos grasos fue analizada por cromatografía de gases. Los valores de IC50 de los extractos fueron 37.09 μg/ml (en el ensayo con DPPH. En el sistema β-carotene/ácido linoleico, el extracto mostró un 65.22% de inhibición frente a la oxidación del ácido linoleico. La cantidad total de contenido fenólico y capacidad antioxidante total fueron 36.52 mg equivalentes de ácido gallico (GAE/g y 74.93 mg equivalentes de ácido ascórbico (AE/g, respectivamente. El principal ácidos graso encontrado, por análisis de CG, en C. kotschyi var. pérsica fue el C 18:3 ω3 (ácido α-linolenico. Los resultados presentados aquí indican que C. kotschyi var. pérsica posee unas fuertes propiedades antioxidantes. Adem

  9. Myenteric neuronal plasticity induced by Toxoplasma gondii (genotype III on the duodenum of rats

    Directory of Open Access Journals (Sweden)

    Rodrigo M. Papazian-Cabanas

    2012-09-01

    Full Text Available The effects of acute and chronic infection caused by Toxoplasma gondii on duodenal myenteric neurons were analyzed. Eighteen rats were assigned into four groups: Acute Control Group (ACG, n=4; Acute Experimental Group (AEG, n=4; Chronic Control Group (CCG, n=5; and Chronic Experimental Group (CEG, n=5. Rats from the AEG and CEG were inoculated orally with 105 genotype III (BTU-II strain tachyzoites of T. gondii isolated from a dog with neurological signs. Acute groups were killed after 24 hours after the inoculation and the chronic groups after 30 days. Whole-mount from the duodenum were stained with Giemsa. The population density of myenteric neurons, as well the body cell, nuclear and cytoplasmic area were analyzed. Both acute and chronic toxoplasmic infection did not provoke neuronal loss. On the other hand, plastic alterations were observed: decreasing of the nuclear and cytoplasmic area during the acute phase and neuronal hypertrophy during the chronic phase.Foram analisados os efeitos da infecção aguda e crônica provocada pelo Toxoplasma gondii sobre os neurônios mientéricos do duodeno. Dezoito ratos foram divididos em quatro grupos: Grupo Controle Agudo (GCA, n= 4, Grupo Experimental Agudo (GEA, n=4, Grupo Controle Crônico (GCC, n=5 e Grupo Experimental Crônico (GEC, n=5. Os animais do GEA e GEC receberam por via oral 10 5 taquizoítos de Toxoplasma gondii da cepa BTUII (genótipo III isolada de um cão com sintomatologia neurológica. Os grupos agudos foram submetidos à eutanásia após 24 horas e os crônicos após 30 dias da inoculação. Preparados totais do duodeno foram corados com Giemsa. A densidade populacional dos neurônios mientéricos, bem como a área do corpo celular, núcleo e citoplasma foram analisados. Ambas, as infecções toxoplásmicas aguda e crônica não provocaram a perda neuronal. Por outro lado, alterações plásticas foram observadas: diminuição da área nuclear e citoplasmática durante a fase

  10. Tripper car 952 - a new machine for Hambach opencast mine; Bandschleifenwagen 952 - ein neues Geraet fuer den Tagebau Hambach

    Energy Technology Data Exchange (ETDEWEB)

    Friebe, J.

    1995-01-01

    In December 1991 Rheinbraun AG placed an order with the firm of TAKRAF Anlagen GmbH in Leipzig for the mechanical equipment of the new tripper car 952. An order for the electrical equipment was placed with AEG. As fas as outer dimensions and operating conditions are concerned, the new tripper car for Hambach opencast mine resembles its predecessors. Significant changes have however been introduced as regards certain details, and to some extent entirely new measures have been adopted, in particular with respect to the travel gear dynamics, the design and dimensions of the steel structure as regards fatigue strength, the belt alignment devices and the noise and dust emission control devices. On other respects, too, this tripper car is an innovation inasmuch as it is the first large opencast machine to be ordered by Rheinbraun directly from a manufacturer in the new Federal states. During construction, which took nearly 3 years, various differences of opinion between the contracting parties became evident as regards order handling, design and construction; the cause of these differences of opinion often only lies in the different developments that took place during the 40 years in which Germany was divided. Thanks to mutual efforts to overcome these differences as quickly as possible, the completion of the new tripper car 952 represents a project that has contributed to cooperation within the entire German brown coal industry and among its contractors. (orig.) [Deutsch] Im Dezember 1991 bestellte die Rheinbraun AG bei der Fa. TAKRAF Anlagen GmbH, Leipzig, den maschinentechnischen Teil des neuen Bandschleifenwagens 952. Fuer die elektrotechnische Ausruestung wurde der Auftrag an AEG vergeben. Der neue Bandschleifenwagen fuer den Tagebau Hambach entspricht in den aeusseren Abmessungen und in den Einsatzbedingungen den Vorgaengergeraeten. Im Detail sind jedoch wesentliche Aenderungen vorgenommen und zum Teil auch neue Wege beschritten worden, insbesondere im Bereich der

  11. Alterations of the myenteric plexus of the ileum and the descending colon caused by Toxoplasma gondii (genotype III Alterações do plexo mientérico do íleo e cólon descendente causadas por Toxoplasma gondii (genótipo III

    Directory of Open Access Journals (Sweden)

    Elaine Yae Yamashita Sugauara

    2008-09-01

    Full Text Available Alterations caused by a genotype III strain of Toxoplasma gondii were assessed with respect to the number and the morphometry of the myenteric neurons in the terminal ileum and the descending colon. Eighteen rats were divided into four groups: Acute Control Group (ACG, n=4; Acute Experimental Group (AEG, n=4; Chronic Control Group (CCG, n=5 and Chronic Experimental Group (CEG, n=5. NaCl solution was administered through gavage to the animals in the ACG and CCG. Toxoplasma gondii tachyzoites (10(4 from a genotype III strain were orally administered to the AEG and CEG. Acute Groups were died after 24 hours, and the Chronic Groups after 30 days. Neuronal loss was not observed in both organs. The neurons atrophied in the terminal ileum as the opposite occurred with the neurons at the descending colon during the chronic phase of infection. In the terminal ileum, the neurons atrophied during the chronic phase of the infection as no alteration was found during the acute phase. For the descending colon, the neurons became hypertrophic during the chronic infection in opposition to the atrophy found during the acute phase.Objetivou-se avaliar as alterações causadas por uma cepa genótipo III de Toxoplasma gondii, sobre o número e a morfometria de neurônios mientéricos, do íleo terminal e do cólon descendente. Dividiu-se dezoitos ratos em quatro grupos: controle agudo (GCA, n=4, experimental agudo (GEA, n=4, controle crônico (GCC, n=5 e experimental crônico (GEC, n=5. Os animais do GCA e GCC receberam solução de NaCl por gavagem, e os animais do GEA e GEC 10(4 taquizoítos de uma cepa genótipo III de T. gondii por via oral. Os grupos agudos após 24 horas foram mortos e os crônicos após 30 dias. Observou-se que não houve perda neuronal em ambos os órgãos. No íleo terminal, os neurônios atrofiaram-se na fase crônica da infecção, enquanto nenhuma alteração ocorreu na fase aguda. Já no cólon descendente, os neurônios tornaram

  12. A high-throughput assay for quantification of starch hydrolase inhibition based on turbidity measurement.

    Science.gov (United States)

    Liu, Tingting; Song, Lixia; Wang, Hongyu; Huang, Dejian

    2011-09-28

    A high-throughput method for rapid determination of starch hydrolase inhibition was developed using a 96-well microplate UV-vis reader to monitor the turbidity decrease over time. The area under the curve of turbidity measured over time was used to quantify the inhibitory effect of polyphenolic compounds on porcine pancreatic amylase, rat intestine α-glucosidase, and fungal amyloglucosidase. Acarbose equivalence (AE) was introduced for the first time and defined as IC50 of acarbose divided by the IC50 of the sample measured under the same 96-well plate. This way, the run-to-run variations are canceled out. Among the plant extracts tested, grape seed extracts (1,440 μmolAE/g) and cinnamon bark extracts (1600 μmolAE/g) are the most active in inhibiting rat intestine α-glucosidase. For porcine α-amylase inhibition, grape seed extracts (5710 μmol AE/g) are close to four times more active (equal weight basis) than acarbose (1550 μmolAE/g).

  13. NATO Advanced Study Institute on Physical Processes in Laser-Materials Interaction, which was the 9th course of the Europhysics School of Quantum Electronics

    CERN Document Server

    1983-01-01

    It is a pleasure to write a few words as an introduction to the proceedings of the 1980 NATO ASI on "Physical Processes in Laser­ Naterial Interaction." This ASI is the ninth course of a series devoted to lasers and their applications, held under the responsibility of the Quantum Electronics Division of the European Physical Society, and for this reason known as the "Europhysics School of Quantum Electronics." Since 1971 the School has been operating with the joint direc­ tion of myself as representative of the academic research, and Dr. D. Roess (formerly with Siemens AEG, Munich, and now with Sick, Optik und Electronik, GmbH, Munich) for the industrial applications. Indeed the aim of the School is to alternate fundamental and applied frontier topics in the area of quantum electronics and modern optics, in order to introduce young research people from universities and industrial R&D laboratories to the new aspects of research opened by the laser.

  14. Me saame hakkama! / Toomas Hendrik Ilves

    Index Scriptorium Estoniae

    Ilves, Toomas Hendrik, 1953-

    2009-01-01

    Vabariigi Presidendi tervitus 2008/2009. aastavahetusel. Ilmunud ka: Postimees 3. jaan. 2009, lk. 3, pealk.: President Toomas Hendrik Ilvese uusaastatervitus; Eesti Päevaleht 3. jaan. 2009, lk. 3, pealk.: Sajandi keerulisim aasta. Vt. samas: Muutunud aeg. Väljavõte presidendi uusaastakõnest 2007. aasta 31. detsembril; Oma Saar 3. jaan. 2009, lk. 5, pealk.: Armas rahvas!; Pärnu Postimees 3. jaan. 2009, lk. 5, pealk.: Vabariigi presidendi aastavahetuse tervitus; Võrumaa Teataja 3. jaan. 2009, lk. 2, pealk.: Vabariigi presidendi tervitus aastavahetusel 2008/2009; Virumaa Teataja 3. jaan. 2009, lk. 11, pealk.: Homne on meie teha; Järva Teataja 3. jaan. 2009, lk. 2, pealk.: Me tuleme toime; Hiiu Leht : Nädalalõpp 9. jaan. 2009, lk. 6, pealk.: Armas rahvas!; Meie Maa 3. jaan. 2009, lk. 2 (tsitaat kõnest); Vaba Eesti Sõna 8. jaan. 2009, lk. 3, pealk.: Vabariigi Presidendi tervitus aastavahetusel; Eesti Päevaleht (Stockholm) 14. jaan. 2009, lk. 1, pealk.: Toomas Hendrik Ilves: Jäägem raskuste kiuste ausaks; Eesti Elu 9. jaan. 2009, lk. 2, pealk.: Vabariigi Presidendi tervitus 2008/2009. aastavahetusel; Meie Kodu 21. jaan. 2009, lk. 1, pealk.: Vabariigi Presidendi tervitus 2008/2009. aastavahetusel; Eesti Hääl 24. jaan. 2009, lk. 1, pealk.: EV Presidendi kõne

  15. The possible correlation between electromagnetic earth fields and future earthquakes

    CERN Document Server

    Mavrodiev, S C

    2001-01-01

    From 23 - 27 July 2001 in the Institute of Nuclear Research and Nuclear Energy, Bulgarian Academy of Sciences, Sofia was held a seminar under the title: "Possible correlations between electromagnetic earth fields and future earthquakes" organized by the Institute's Department of "Astrophysical Objects and Environment". As a main result of the first two days seminar work was the prediction of the direction and the day of the Skyros, Greece earthquake, that was confirned in the next day: Aeg. Sea, 07/26 00:21:37, 39.06N 24.34E, Depth 19 km, Mag 6.3- http://www.noa.gr/English/home-en.html. Analogously, a month later the day of Yambol, Bulgaria eartquake was predicted. The papers of A.M.Galper et al (2001), "High- energy charged particle flux variations in vicinity of Earth as earthquakes precursors", http://www.pereplet.ru/pops/quakes.html, and http://science.rambler.ru/db/msg.html?mid=1168269&uri=galper.html, the analysis of NASA data from http://www.sel.noaa.gov, explained why the Seminar main hypothesis w...

  16. Fragmente "Lüürikast" / Wendy Saloman ; inglise keelest tlk. Doris Kareva

    Index Scriptorium Estoniae

    Saloman, Wendy

    2001-01-01

    Sisu : "Kannikesed olid kreeklaste surmalilled..."; "Foto kannikestest on hakanud äärtest keerduma. Leidsid nad lumest sel..."; "Kujutle Isist kogumas surnud Osirise tükke..."; "Kujutlus on igaviku tööriist..."; "Mäletada tuba tähendab mäletada mis tahes tuba hommikuvalgusega läbi..."; "Aeg muutis su ujedaks. Eebenipuust kell kaminasimsil meenutas sulle..."; "Kuidas saaksin mäletada tuba, mäletamata su puudumist..."; "Mälestus uhub üle su kuju nagu valguse mäng veel. Ometi, kui mõtlen..."; "Armastasime võimalust endis..."; "Kummaline on meenutada käte intiimsust: hoidjad ja avastajad, sõnade..."; "Vahel öeldakse, et kaamera on surma tööriist..."; "Nostalgia koondub su kujus..."; "Tamariskipuu hõrk hõng tõmbas Isise Byblosse..."; "Aprillis on hetki, mil koobaltsinine taevas on südame saadik..."; "Ja mõnegi meelest on Mercurius kaamera vaim ja fotograafia alkeemia..."; Isis öeldakse olevat muusadest esimene..."; "Suveregati kiirusel ummistab mälu Herakleitose jõe..."; "Möödunud ööl üllatasid mind unes..."; "Sõnad kargavad meelde, kaovad, kujutud fragmendid igavesest kogemusest..."

  17. A Radiation Service Centre for Research and Large-Scale Irradiation

    International Nuclear Information System (INIS)

    In the near future radiation processing of food may change from the present laboratoryscale to large industrial application. This step will require large irradiation facilities with high flexibility, a safe dose control system and simple food-handling systems. Some design parameters of such an irradiation facility have already been realized at the AEG-Telefunken Radiation Service Centre in Wedel. This centre came into operation in autumn 1976. It is equipped with one research-type high-power X-ray unit (200 kV/32 mA) and one industrial-type Dynamitron® electron accelerator (1500 kV/37.5 kW). Handling systems are available for radiation crosslinking of wire and cable insulations, of plastic films, for irradiation treatment of components and parts of different types and coatings as also of sewage sludge and waste water. Some of these handling systems can be used for food irradiation too. Other handling systems will be added sometime later. As an additional service the Company's existing material and environmental testing laboratory will be available. The centre is already being used by many interested companies to investigate the effects of radiation on a broad range of organic and inorganic materials, to develop special processing equipment, to process supplied products and to perform R and D work and contracts. The service centre fills an existing gap and will have an impact on the commercialization of radiation processing techniques in Europe. (author)

  18. Space Power Program Semiannual Progress Report for period ending June 30, 1963

    Energy Technology Data Exchange (ETDEWEB)

    Miller, A. J. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    1963-10-11

    This is a report of progress on the Oak Ridge National Laboratory's research and development program on nuclear power plants for electrical power production in space vehicles. The work is carried out under AEG Reactor Experiments, Fuels, and Materials, and Reactor Component programs. Research and development work is under way on the stainless steel boiling-potassium reactor and the Medium Power Reactor Experiment, boiling alkali metal heat transfer, high-temperature and refractory alloys, fuel material, and space reactor shielding, particularly in connection with SNAP 2, 8, 10, and 50. Many of these OREL efforts are directed toward the development of a specific type of power plant, but they also furnish a significant contribution of scientific and engineering information needed in other programs on advanced SNAP systems. Progress on research and development directly related to the Medium Power Reactor Experiment (MPRE) is presented mostly in Part I of this report. Progress on the MPRE will, in the future, be reported on a quarterly basis. The form of the reporting will alternate from MPRE Quarterly Progress Reports to Space Power Semiannual Progress Reports.

  19. Vikergallup : eesti kirjandus 2008 / Peeter Helme, Ott Kilusk, Igor Kotjuh... [jt.

    Index Scriptorium Estoniae

    2009-01-01

    21 arvustaja vastus küsimusele, milline oli 2008. aasta parim uudisteos ja debüüt. Ära nimetati fs-i luulekogu "Alasti ja elus", Andrei Hvostovi proosaraamatut "Võõrad lood", Maarja Kangro luulekogu "Heureka", Kalju Kruusa luulekogu "Pilvedgi mindgi liigutavadgi", Asko Künnapi luulekogu "Su ööd on loetud", Elo Viidingu proosaraamatut "Püha Maama", Mart Juure luulekogu "Pane Eesti peale tagasi", Jaan Kaplisnki luulekogu "Teiselpool järve", Mart Kivastiku proosaraamatut "Kurb raamat", Leo Kunnase romaani "Gort Ashryn I", Andres Langemetsa luulekogu "Vooder", Erkki Luugi "Valitud luuletusi", Rein Põdra romaani "Juba olnud", Rein Raua lühiromaani "Vend" ja Jaan Tangsoo romaani "Méduse'i parv". Parima debüüdina mainiti Andrus Kasemaa luulekogu "Poeedirahu", Barthol Lo Mejori proosaraamatut "Popdada" ja Chaneldiori proosaraamatut "Kontrolli alt väljas", Lauri Saatpalu laulutekstiraamatut "Nähtused ja tehtused", Tamur Kusnetsi romaani "Hundipäikese aeg" ja Jaanika Merilo luulekogu "Rütmist väljas"

  20. Accuracy-Energy Configurable Sensor Processor and IoT Device for Long-Term Activity Monitoring in Rare-Event Sensing Applications

    Directory of Open Access Journals (Sweden)

    Daejin Park

    2014-01-01

    Full Text Available A specially designed sensor processor used as a main processor in IoT (internet-of-thing device for the rare-event sensing applications is proposed. The IoT device including the proposed sensor processor performs the event-driven sensor data processing based on an accuracy-energy configurable event-quantization in architectural level. The received sensor signal is converted into a sequence of atomic events, which is extracted by the signal-to-atomic-event generator (AEG. Using an event signal processing unit (EPU as an accelerator, the extracted atomic events are analyzed to build the final event. Instead of the sampled raw data transmission via internet, the proposed method delays the communication with a host system until a semantic pattern of the signal is identified as a final event. The proposed processor is implemented on a single chip, which is tightly coupled in bus connection level with a microcontroller using a 0.18 μm CMOS embedded-flash process. For experimental results, we evaluated the proposed sensor processor by using an IR- (infrared radio- based signal reflection and sensor signal acquisition system. We successfully demonstrated that the expected power consumption is in the range of 20% to 50% compared to the result of the basement in case of allowing 10% accuracy error.

  1. FameLab International Final: a triumph for Switzerland and CERN

    CERN Multimedia

    CERN Bulletin

    2015-01-01

    Oskari Vinko, from ETH Zurich, who won the Swiss finals organised at CERN last May, is the winner of the 2015 FameLab competition. The CERN winner Lillian Smestad, member of the AEgIS collaboration from the Norwegian Research Council, shared second place with François-Xavier Joly from France, who also was among the trainees at the FameLab Master Classes organised by CERN in April.     CERN's Lillian Smestad (left) and Oskari Vinko from ETH Zurich, during the Swiss and CERN joint final, organised at CERN in May 2015. The 2015 FameLab International Final, which took place on 4 June in Cheltenham, UK, was a triumph for Switzerland and CERN! The competition was very hard this year, with 27 countries participating in the international semifinals and only nine making it through to the finals. This was also the first year that CERN took part as a “country”.  Congratulations to all the winners! To experience the international Famelab ...

  2. Direct detection of antiprotons with the Timepix3 in a new electrostatic selection beamline

    Science.gov (United States)

    Pacifico, N.; Aghion, S.; Alozy, J.; Amsler, C.; Ariga, A.; Ariga, T.; Bonomi, G.; Bräunig, P.; Bremer, J.; Brusa, R. S.; Cabaret, L.; Caccia, M.; Campbell, M.; Caravita, R.; Castelli, F.; Cerchiari, G.; Chlouba, K.; Cialdi, S.; Comparat, D.; Consolati, G.; Demetrio, A.; Di Noto, L.; Doser, M.; Dudarev, A.; Ereditato, A.; Evans, C.; Ferragut, R.; Fesel, J.; Fontana, A.; Gerber, S.; Giammarchi, M.; Gligorova, A.; Guatieri, F.; Haider, S.; Holmestad, H.; Huse, T.; Jordan, E.; Kellerbauer, A.; Kimura, M.; Krasnický, D.; Lagomarsino, V.; Lansonneur, P.; Lawler, G.; Lebrun, P.; Llopart, X.; Malbrunot, C.; Mariazzi, S.; Marx, L.; Matveev, V.; Mazzotta, Z.; Nebbia, G.; Nedelec, P.; Oberthaler, M.; Pagano, D.; Penasa, L.; Petracek, V.; Pistillo, C.; Prelz, F.; Prevedelli, M.; Ravelli, L.; Resch, L.; Røhne, O. M.; Rotondi, A.; Sacerdoti, M.; Sandaker, H.; Santoro, R.; Scampoli, P.; Smestad, L.; Sorrentino, F.; Spacek, M.; Storey, J.; Strojek, I. M.; Testera, G.; Tietje, I.; Tlustos, L.; Widmann, E.; Yzombard, P.; Zavatarelli, S.; Zmeskal, J.; Zurlo, N.

    2016-09-01

    We present here the first results obtained employing the Timepix3 for the detection and tagging of annihilations of low energy antiprotons. The Timepix3 is a recently developed hybrid pixel detector with advanced Time-of-Arrival and Time-over-Threshold capabilities and has the potential of allowing precise kinetic energy measurements of low energy charged particles from their time of flight. The tagging of the characteristic antiproton annihilation signature, already studied by our group, is enabled by the high spatial and energy resolution of this detector. In this study we have used a new, dedicated, energy selection beamline (GRACE). The line is symbiotic to the AEgIS experiment at the CERN Antiproton Decelerator and is dedicated to detector tests and possibly antiproton physics experiments. We show how the high resolution of the Timepix3 on the Time-of-Arrival and Time-over-Threshold information allows for a precise 3D reconstruction of the annihilation prongs. The presented results point at the potential use of the Timepix3 in antimatter-research experiments where a precise and unambiguous tagging of antiproton annihilations is required.

  3. Antioxidant capacity and fatty acid profile of Centaurea kotschyi (Boiss. and Heldr.) Hayek var. persica (Boiss.) Wagenitz from Turkey

    Energy Technology Data Exchange (ETDEWEB)

    Zengin, G.; Guler, G.O.; Cakmak, Y.S.; Aktumseka, A.

    2011-07-01

    The antioxidant capacity of the methanolic extract and the fatty acid composition of C. kotschyi var. persica were investigated. Six different chemical methods were used to determine the antioxidant capacity. The fatty acid composition was analyzed using gas chromatography. The IC50 value of the extract was determined as 37.09 ig/ml (in the DPPH assay). In the {beta}carotene/linoleic acid system, the extract exhibited 65.22% inhibition against linoleic acid oxidation. The amount of total phenolic content and total antioxidant capacity were detected as 36.52 mg gallic acid equivalent (GAE)/g and 74.93 mg ascorbic acid equivalent (AE)/g, respectively. The major fatty acid in the composition of C. kotschyi var. persica was found to be C 18:3 u3 ({beta}-linolenic acid) by GC analysis. The results presented here indicate that C. kotschyi var. persica possess strong antioxidant properties. Therefore, the species can be used as a natural additive in food, cosmetic and pharmaceutical industries. (Author).

  4. Accuracy-energy configurable sensor processor and IoT device for long-term activity monitoring in rare-event sensing applications.

    Science.gov (United States)

    Park, Daejin; Cho, Jeonghun

    2014-01-01

    A specially designed sensor processor used as a main processor in IoT (internet-of-thing) device for the rare-event sensing applications is proposed. The IoT device including the proposed sensor processor performs the event-driven sensor data processing based on an accuracy-energy configurable event-quantization in architectural level. The received sensor signal is converted into a sequence of atomic events, which is extracted by the signal-to-atomic-event generator (AEG). Using an event signal processing unit (EPU) as an accelerator, the extracted atomic events are analyzed to build the final event. Instead of the sampled raw data transmission via internet, the proposed method delays the communication with a host system until a semantic pattern of the signal is identified as a final event. The proposed processor is implemented on a single chip, which is tightly coupled in bus connection level with a microcontroller using a 0.18 μm CMOS embedded-flash process. For experimental results, we evaluated the proposed sensor processor by using an IR- (infrared radio-) based signal reflection and sensor signal acquisition system. We successfully demonstrated that the expected power consumption is in the range of 20% to 50% compared to the result of the basement in case of allowing 10% accuracy error.

  5. Mathematical Descriptions of Axially Varying Penning Traps for the Antimatter Experiment: gravity, Interferometry, and Spectroscopy

    CERN Document Server

    Stephanie, Brown

    2015-01-01

    Antimatter, though proposed in 1933, is still not well understood. AEgIS aims to study the interaction of antihydrogen with the earth's gravitational field. This information will add to our understanding of the matter-antimatter asymmetry present in our universe. This paper discusses a Penning-Malmberg with a magnetic mirror that will hold $C_{2}^{-}$ that will be used for sympathetic cooling of antiprotons before the antihydrogen is created. The trap, which is critical to the cooling process of the antihydrogen, can be characterized by the separatrix between trapped and untrapped particles. This paper applies analytical processes used to define the separatrix of pure electron plasmas to a molecular plasma. Our work is based on the desire conditions (density, particle number, field strength, trap size) of the high field region. The initial application of a semi-analytical method applied to our trap defines the trap potential difference at \\~ 0.6V. The separatrix is defined in both the high and low fiel...

  6. The transcription factor VvWRKY33 is involved in the regulation of grapevine (Vitis vinifera) defense against the oomycete pathogen Plasmopara viticola.

    Science.gov (United States)

    Merz, Patrick R; Moser, Tina; Höll, Janine; Kortekamp, Andreas; Buchholz, Günther; Zyprian, Eva; Bogs, Jochen

    2015-03-01

    Grapevine (Vitis vinifera ssp. vinifera) is one of the most important fruit species; however, it is highly susceptible to various pathogens, which can cause severe crop losses in viticulture. It has been shown that several WRKY class transcription factors (TFs) are part of the signal transduction cascade, which leads to the activation of plant defense reactions against various pathogens. In the present investigation, a full-length cDNA was isolated from V. vinifera leaf tissue encoding a predicted protein, designated VvWRKY33, which shows the characteristics of group I WRKY protein family. VvWRKY33 induction correlates with the expression of VvPR10.1 (pathogenesis-related 10.1) gene in the leaves of the resistant cultivar 'Regent' after infection with Plasmopara viticola, whereas in the susceptible cultivar 'Lemberger' VvWRKY33 and VvPR10.1 are not induced. Corresponding expression of the TF and VvPR10.1 was even obtained in uninfected ripening berries. In planta, analysis of VvWRKY33 has been performed by ectopic expression of VvWRKY33 in grapevine leaves of greenhouse plants mediated via Agrobacterium tumefaciens transformation. In consequence, VvWRKY33 strongly increases resistance to P. viticola in the susceptible cultivar 'Shiraz' and reduces pathogen sporulation of about 50-70%, indicating a functional role for resistance in grapevine. Complementation of the resistance-deficient Arabidopsis thaliana Columbia-0 (Col-0) mutant line wrky33-1 by constitutive expression of VvWRKY33 restores resistance against Botrytis cinerea to wild-type level and in some complemented mutant lines even exceeds the resistance level of the parental line Col-0. Our results support the involvement of VvWRKY33 in the defense reaction of grapevine against different pathogens. PMID:25132131

  7. The transcription factor VvWRKY33 is involved in the regulation of grapevine (Vitis vinifera) defense against the oomycete pathogen Plasmopara viticola.

    Science.gov (United States)

    Merz, Patrick R; Moser, Tina; Höll, Janine; Kortekamp, Andreas; Buchholz, Günther; Zyprian, Eva; Bogs, Jochen

    2015-03-01

    Grapevine (Vitis vinifera ssp. vinifera) is one of the most important fruit species; however, it is highly susceptible to various pathogens, which can cause severe crop losses in viticulture. It has been shown that several WRKY class transcription factors (TFs) are part of the signal transduction cascade, which leads to the activation of plant defense reactions against various pathogens. In the present investigation, a full-length cDNA was isolated from V. vinifera leaf tissue encoding a predicted protein, designated VvWRKY33, which shows the characteristics of group I WRKY protein family. VvWRKY33 induction correlates with the expression of VvPR10.1 (pathogenesis-related 10.1) gene in the leaves of the resistant cultivar 'Regent' after infection with Plasmopara viticola, whereas in the susceptible cultivar 'Lemberger' VvWRKY33 and VvPR10.1 are not induced. Corresponding expression of the TF and VvPR10.1 was even obtained in uninfected ripening berries. In planta, analysis of VvWRKY33 has been performed by ectopic expression of VvWRKY33 in grapevine leaves of greenhouse plants mediated via Agrobacterium tumefaciens transformation. In consequence, VvWRKY33 strongly increases resistance to P. viticola in the susceptible cultivar 'Shiraz' and reduces pathogen sporulation of about 50-70%, indicating a functional role for resistance in grapevine. Complementation of the resistance-deficient Arabidopsis thaliana Columbia-0 (Col-0) mutant line wrky33-1 by constitutive expression of VvWRKY33 restores resistance against Botrytis cinerea to wild-type level and in some complemented mutant lines even exceeds the resistance level of the parental line Col-0. Our results support the involvement of VvWRKY33 in the defense reaction of grapevine against different pathogens.

  8. The landscape of cytokinin binding by a plant nodulin

    Energy Technology Data Exchange (ETDEWEB)

    Ruszkowski, M.; Szpotkowski, K.; Sikorski, M. [Polish Academy of Sciences, Poznan (Poland); Jaskolski, M., E-mail: mariuszj@amu.edu.pl [Polish Academy of Sciences, Poznan (Poland); A. Mickiewicz University, Poznan (Poland)

    2013-12-01

    The crystal structures of complexes of M. truncatula nodulin 13 with four cytokinins, trans-zeatin, N{sup 6}-isopentenyladenine, kinetin and N{sup 6}-benzyladenine, show an unusual mode of dimerization of this PR-10-fold plant protein. The cytokinin-binding mode in the internal cavity of the protein is the same in each complex and resembles the pattern found in the cytokinin receptor protein. Nodulation is an extraordinary symbiotic interaction between leguminous plants and nitrogen-fixing bacteria (rhizobia) that assimilate atmospheric nitrogen (in root nodules) and convert it into compounds suitable for the plant host. A class of plant hormones called cytokinins are involved in the nodulation process. In the model legume Medicago truncatula, nodulin 13 (MtN13), which belongs to the pathogenesis-related proteins of class 10 (PR-10), is expressed in the outer cortex of the nodules. In general, PR-10 proteins are small and monomeric and have a characteristic fold with an internal hydrophobic cavity formed between a seven-stranded antiparallel β-sheet and a C-terminal α-helix. Previously, some PR-10 proteins not related to nodulation were found to bind cytokinins such as trans-zeatin. Here, four crystal structures of the MtN13 protein are reported in complexes with several cytokinins, namely trans-zeatin, N{sup 6}-isopentenyladenine, kinetin and N{sup 6}-benzyladenine. All four phytohormones are bound in the hydrophobic cavity in the same manner and have excellent definition in the electron-density maps. The binding of the cytokinins appears to be strong and specific and is reinforced by several hydrogen bonds. Although the binding stoichiometry is 1:1, the complex is actually dimeric, with a cytokinin molecule bound in each subunit. The ligand-binding site in each cavity is formed with the participation of a loop element from the other subunit, which plugs the only entrance to the cavity. Interestingly, a homodimer of MtN13 is also formed in solution, as confirmed

  9. Promotion effect of cobalt-based catalyst with rare earth for the ethanol steam reforming

    Science.gov (United States)

    Chiou, Josh Y. Z.; Chen, Ya-Ping; Yu, Shen-Wei; Wang, Chen-Bin

    2013-12-01

    Catalytic performance of ethanol steam reforming (ESR) was investigated on praseodymium (Pr) modified ceria-supported cobalt oxide catalyst. The ceria-supported cobalt oxide (Ce-Co) catalyst was prepared by co-precipitation-oxidation (CPO) method, and the doped Pr (5 and 10 wt% loading) catalysts (Pr5-Ce-Co and Pr10-Ce-Co) were prepared by incipient wetness impregnation method. The reduction pretreatment under 250 and 400 °C (H250 and H400) was also studied. All samples were characterized by XRD, TPR and TEM. Catalytic performance of ESR was tested from 250 to 500 °C in a fixed-bed reactor. The doping of Pr into the ceria lattice has significantly promoted the activity and reduced the coke formation. The products distribution also can be influenced by the different reduction pretreatment. The Pr10-Ce-Co-H400 sample is a preferential ESR catalyst, where the hydrogen distribution approaches 73% at 475 °C with less amounts (< 2%) of CO and CH4.

  10. CO and Soot Oxidation over Ce-Zr-Pr Oxide Catalysts.

    Science.gov (United States)

    Andana, Tahrizi; Piumetti, Marco; Bensaid, Samir; Russo, Nunzio; Fino, Debora; Pirone, Raffaele

    2016-12-01

    A set of ceria, ceria-zirconia (Ce 80 at.%, Zr 20 at.%), ceria-praseodymia (Ce 80 at.%, Pr 20 at.%) and ceria-zirconia-praseodymia (Ce 80 at.%, Zr 10 at.% and Pr 10 at.%) catalysts has been prepared by the solution combustion synthesis (SCS). The effects of Zr and Pr as dopants on ceria have been studied in CO and soot oxidation reactions. All the prepared catalysts have been characterized by complementary techniques, including XRD, FESEM, N2 physisorption at -196 °C, H2-temperature-programmed reduction, and X-ray photoelectron spectroscopy to investigate the relationships between the structure and composition of materials and their catalytic performance. Better results for CO oxidation have been obtained with mixed oxides (performance scale, Ce80Zr10Pr10 > Ce80Zr20 > Ce80Pr20) rather than pure ceria, thus confirming the beneficial role of multicomponent catalysts for this prototypical reaction. Since CO oxidation occurs via a Mars-van Krevelen (MvK)-type mechanism over ceria-based catalysts, it appears that the presence of both Zr and Pr species into the ceria framework improves the oxidation activity, via collective properties, such as electrical conductivity and surface or bulk oxygen anion mobility. On the other hand, this positive effect becomes less prominent in soot oxidation, since the effect of catalyst morphology prevails. PMID:27255898

  11. Prospecting plant growth promoting bacteria and cyanobacteria as options for enrichment of macro- and micronutrients in grains in rice–wheat cropping sequence

    Directory of Open Access Journals (Sweden)

    Anuj Rana

    2015-12-01

    Full Text Available The influence of plant growth promoting bacteria (PGPB and cyanobacteria, alone and in combination, was investigated on micronutrient enrichment and yield in rice–wheat sequence, over a period of two years. Analysis of variance (ANOVA in both crops indicated significant differences in soil dehydrogenase activity and micronutrient enrichment in grains (Fe, Zn in rice, and Cu, Mn in wheat. The combined inoculation of Anabaena oscillarioides CR3, Brevundimonas diminuta PR7, and Ochrobactrum anthropi PR10 (T6 significantly increased nitrogen, phosphorus, and potassium (NPK content and improved rice yield by 21.2%, as compared to the application of recommended dose of NPK fertilizers (T2. The treatment T5 (Providencia sp. PR3 + B. diminuta PR7 + O. anthropi PR10 recorded an enhancement of 13–16% in Fe, Zn, Cu, and Mn concentrations, respectively, in rice grains. In wheat, Providencia sp. PW5 (T6 recorded the highest yield (5.23 Mg ha−1 and significantly higher enrichment of Fe and Cu (44–45% in the grains. This study highlighted the promise of combinations of cyanobacteria/bacteria and their synergistic action in biofortification and providing savings of 40–60 kg N ha−1. Future focus needs to be towards integrating such promising environment-friendly and environmentally sustainable options in nutrient management strategies for this cropping sequence.

  12. Effects of temperature during soybean seed development on defense-related gene expression and fungal pathogen accumulation.

    Science.gov (United States)

    Upchurch, Robert G; Ramirez, Martha E

    2011-12-01

    Soybean [Glycine max (L.) Merr] plants were exposed to three temperature regimens during seed development to investigate the effect of temperature on the expression of eight defense-related genes and the accumulation of two fungal pathogens in inoculated seeds. In seeds prior to inoculation, either a day/night warm (34/26 °C) or a cool temperature (22/18 °C) relative to normal (26/22 °C) resulted in altered patterns of gene expression including substantially lower expression of PR1, PR3 and PR10. After seed inoculation with Cercospora kikuchii, pathogen accumulation was lowest in seeds produced at 22/18 °C in which of all defense genes, MMP2 was uniquely most highly induced. For seeds inoculated with Diaporthe phaseolorum, pathogen accumulation was lowest in seeds produced at 34/26 °C in which of all defense genes, PR10 was uniquely most highly induced. Our detached seed assays clearly demonstrated that the temperature regimens we applied during seed development produced significant changes in seed defense-related gene expression both pre- and post inoculation and our findings support the hypothesis that global climate change may alter plant-pathogen interactions and thereby potentially crop productivity.

  13. Screening Rice Cultivars for Resistance to Bacterial Leaf Blight.

    Science.gov (United States)

    Fred, Agaba Kayihura; Kiswara, Gilang; Yi, Gihwan; Kim, Kyung-Min

    2016-05-28

    Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious threats to rice production. In this study, screening of rice for resistance to BLB was carried out at two different times and locations; that is, in a greenhouse during winter and in an open field during summer. The pathogenicity of Xoo race K1 was tested on 32 Korean rice cultivars. Inoculation was conducted at the maximum tillering stage, and the lesion length was measured after 14 days of inoculation. Five cultivars, Hanareum, Namcheon, Samgdeok, Samgang, and Yangjo, were found to be resistant in both the greenhouse and open-field screenings. Expression of the plant defense-related genes JAmyb, OsNPR1, OsPR1a, OsWRKY45, and OsPR10b was observed in resistant and susceptible cultivars by qRT-PCR. Among the five genes tested, only OsPR10b showed coherent expression with the phenotypes. Screening of resistance to Xoo in rice was more accurate when conducted in open fields in the summer cultivation period than in greenhouses in winter. The expression of plant defenserelated genes after bacterial inoculation could give another perspective in elucidating defense mechanisms by using both resistant and susceptible individuals. PMID:26869604

  14. Development of integrated superconducting devices for signal preprocessing. Final report; Entwicklung supraleitender Bausteine der Signalvorverarbeitung. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Biehl, M.; Koch, R.; Neuhaus, M.; Scherer, T.; Jutzi, W.

    1998-02-01

    SPICE and CADENCE based tools for designing, simulating and optimizing SFQ and RSFQ circuits have been developed as well as a standard cell library corresponding to the fabrication technology established at the IEGI. A 12 bit flux shuttle shift register using Nb/Al Josephson junctions with a new write and readout gate has been fabricated and tested successfully; the power dissipation is 9 nW/bit/GHz. A pseudo random pulse generator was developed correspondingly. Simulations of RSFQ toggle flipflops during a large number of clock cycles demonstrated that the digital performance of counters is limited to clock frequencies below 100 GHz by dynamic effects, especially of parasitic inductances. Therefore dc measurements based on the voltage-frequency Josephson relationship must be followed by real time measurements of single SFQ word pulses. A four stage Nb based RSFQ counter in a coplanar waveguide test jig was tested up to a frequency of 2 GHz, limited by the available 32 bit pattern generator and the bandwith of the sampling oscilloscope, yielding bit error rates of about 10{sup -12}. Using YBCO technology, a 4 bit SFQ shift register (T=40 K) as well as miniaturized coplanar microwave devices for satellite and communication applications at 10 GHz (T=77 K) have been designed and fabricated. A 4 bit instantaneous real time frequency meter (IFM) and a microwave filter with a 3-dB bandwidth of only 1.8% have been mounted on the cold head of a split-cycle Stirling cooler (AEG, 1.5 W rate at 80 K) and tested successfully. Hybrid devices, e.g. amplifiers and oscillators, combining active semiconductor components and low loss coplanar YBCO transmission lines operated at 77 K seem very promising. (orig.) [Deutsch] Werkzeuge der Auslegung, Simulation und Optimierung von SFQ- und RSFQ-Schaltungen auf der Basis von SPICE und CADENCE wurden am IEGI entwickelt und eingesetzt. Eine auf die Technologien des Instituts zugeschnittene Bibliothek von Zellen ist vorhanden. Mit der

  15. Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation

    Institute of Scientific and Technical Information of China (English)

    Debora J. Cohen; Vanina G. Da Ros; Dolores Busso; Diego A. Ellerman; Julieta A. Maldera; Nadia Goldweic; Patricia S. Cuasnicú

    2007-01-01

    Rat protein DE is an androgen-dependent cysteine-rich secretory protein (CRISP) synthesized by proximal epididymal regions. DE, also known as CRISP- 1, is localized on the equatorial segment of acrosome-reacted spermatozoa and participates in gamete fusion through binding to egg complementary sites. Immunization of rats with DE inhibits fertility and sperm fusion ability, suggesting that DE represents a good epididymal contraceptive target.Recombinant DE fragments and synthetic peptides revealed that DE binds to the egg via a 12-amino acid region of an evolutionarily conserved motif, Signature 2 (S2). The ability of other CRISP to bind to the rat egg was correlated with their S2 amino acid sequences. Although testicular protein Tpx-1 (CRISP-2) was capable of binding to rodent eggs,human epididymal AEG-related protein (ARP) and helothermine (from lizard saliva) were not. The S2 region presented only two substitutions in Tpx-1 and four in ARP and helothermine, compared with the DE S2, suggesting that this amino acid sequence was relevant for egg interaction. Studies with Tpx-1 and anti-Tpx-1 revealed the participation of this protein in gamete fusion through binding to complementary sites in the egg. In competition studies, DE reduced binding of Tpx- 1 dose-dependently, indicating that both CRISP share the egg complementary sites. That anti-DE and anti-Tpx-1 inhibit sperm-egg fusion while recognizing only the corresponding proteins, suggests functional cooperation between these homologous CRISP to ensure fertilization success. These results increase our understanding of the molecular mechanisms of gamete fusion and contribute to the development of new and safer fertility regulating methods.

  16. Mu isamaa on minu arm : [luuletused] / Lydia Koidula ; [Impressioonid]: Viiu Härm

    Index Scriptorium Estoniae

    Koidula, Lydia, pseud., 1843-1886

    2004-01-01

    Sisu: Mu isamaa on minu arm! ; Eestimaa ; Eestimaa, su hiilgav rada ; Õpetus ; Veel! ; Ülesse unest! ; Ta aina sirgub usalduse rinnal ; Igatsus ; Isamaast ikka! ; Õige! ; Halasta! ; Teretus ; See on su isamaa! ; Üks ; Kaebus ; Kallim kingitus ; Eesti naine ; Kodu ; Miks sa nutad? ; Jutt ; Puhtad pihud ; Mul seisab meeles alati ; Ei jäänud ; Üht eesti laulu ; Mu isamaa, nad olid matnud ; Üksmeel ; Neid austa! ; Emajõe kaldal ; Eesti kotkas ; Sind surmani ; Tulge! ; Eesti muld ja eesti süda ; Sinu külge! ; Isamaa, usk, rahvas! ; Laulu sisu ; Tule! ; Teretus ; Jälle isamaa! ; Mõtted Toomemäel ; Ära looda, et sind usun ; Pühapäeva kellad ; Kosjakased ; Kaks selget silma ; Kõik muutunud ; Kase varjul kogemata ; Laulupisarad ; Kui ta teaks ; Kui vähe! ; Kadunud ; Laulu allikas ; Kaljukiri ; Näed kõrgeid pilvi - ; Ööbik ; Pikalt kiigub paadike ; Tee lahti! ; Aeg ; Ilu ; Noormehe igatsus ; Kolm sõna ; Hoia oma õnne! ; Kui sa kuulsid ; Igatsus ; Haual ; Kurtes ; Neiu kanga juures ; Hällilaul ; Õnn ; Südame põhjas ; Ülejäänud ; Puhkama ; Õhtul ; Lootus ; Lähedal ; Ema süda ; Saeveski ; Sügisemõtteid ; Kasepuu ; Kevade on sala tulnud ; Kuu ja künnilind ; Linnu lennul ; Ööbik ; Õhtu rahu ; Kuningas kevade ; Tühi pesa ; Järv ; Täis laulu ; Õhtu pühitsus ; Rüütli mõrsja ; Narva neitsi ; Akadeemik dr. F. J. Wiedemann'ile ; Sõber ; Palu andeks! ; Kuningatel kümme kinki ; Tasa, tasa! ; Küsimused ; Mälestus ; Koidula õnn ; Ümber ühe ilmakera; Ise ; Enne surma Eestimaale

  17. "Me oma kõrgust eal ei tea..." : [luuletused] / Emily Dickinson ; tlk. Doris Kareva

    Index Scriptorium Estoniae

    Dickinson, Emily

    2006-01-01

    Sisu: "Me oma kõrgust eal ei tea..." ; "Ma tulen! - silme ees..." ; "Et mina ei saand peatuda..." ; "Surm see on kahekõne vaid..." ; "Ma surin Ilu eest - ent Kirst..." ; "Olen Eikeegi. Ja sina..." ; "Ei pälvi kuigivõrd..." ; "Kas võib tõesti tulla Hommik..." ; "Lilled - noh, kui ükski suudaks..." ; "Kui kogu elu kurbused..." ; "Ei eales enam - Tavalist..." ; "Üht kaotust igavesti kandsin..." ; "Suur Lootus langes..." ; "Kui säästa saan üht Surmavalu..." ; "See linnuke on Lootus..." ; "Ma olin Aastaid nälginud..." ; "Maist õnne magusaimaks..." ; "Kui Räitsakas..." ; "Ma olin Maja mannetuim..." ; "Ma elan Võimaluses..." ; "Hing valib Seltsi ainuomase..." ; "On tühja taeva üksildus..." ; "Need, kes on hauas viibinud kaua..." ; "Haud haljas Roheline on..." ; "See lõplikkusetunne..." ; "Muud Elu pole mul..." ; "Oo öö! Oo Öö..." ; "Ei söanda sõbrast lahkuda..." ; "Küll kõigest kõnelesime..." ; "Ta suri - nii ta surigi..." ; "Mis kartsime see tuligi..." ; "Kui sureb sõber kellelgi..." ; "Surm - võtab vaevalt viivu..." ; ""Aeg ravib haavad" öeldakse..." ; "Jah - nende tabamatus..." ; "Hää vaadata on tagasi..." ; "On Elu ja on Eluvorm..." ; "Hõiska - suur torm on möödas..." ; "Septembri Kandidaadikraad..." ; "Ma mõõdan iga Kannatust..." ; "Silm harjub Pimedusega..." ; "Kaks korda elu sulges end..." ; "Kõik ütle, kõik kuid kõverdi..." ; "Ma kardan Meest kel napp on Jutt..." ; "Kes võitleb üksi rahva ees..." ; "Võib näida vaieldamatu..." ; "On uppumisest armetum..." ; "Kergelt astus kõrge täht..." ; "Tee Värav valla, Surm! ; "See on mu kiri ilmale...". Eluloolisi andmeid autori kohta lk. 767

  18. Design and characterizations of two novel cellulases through single-gene shuffling of Cel12A (EG3) gene from Trichoderma reseei.

    Science.gov (United States)

    Yenenler, Aslı; Sezerman, Osman Uğur

    2016-06-01

    Cellulases have great potential to be widely used for industrial applications. In general, naturally occurring cellulases are not optimized and limited to meet the industrial needs. These limitations lead to demand for novel cellulases with enhanced enzymatic properties. Here, we describe the enzymatic and structural properties of two novel enzymes, EG3_S1 and EG3_S2, obtained through the single-gene shuffling approach of Cel12A(EG3) gene from Trichoderma reseei EG3_S1 and EG3_S2 shuffled enzymes display 59 and 75% identity in protein sequence with respect to native, respectively. Toward 4-MUC, the minimum activity of EG3_S1 was reported as 5.9-fold decrease in native at 35°C, whereas the maximum activity of EG3_S2 was reported as 15.4-fold increase in native activity at 40°C. Also, the diminished enzyme activity of EG3_S1 was reported within range of 0.6- to 0.8-fold of native and within range of 0.5- to 0.7-fold of native toward CMC and Na-CMC, respectively. For EG3_S2 enzyme, the improved enzymatic activities within range of 1.1- to 1.4-fold of native and within range of 1.1- to 1.6-fold of native were reported toward CMC and Na-CMC, respectively. Moreover, we have reported 6.5-fold increase in the kcat/Km ratio of EG3_S2 with respect to native and suggested EG3_S2 enzyme as more efficient catalysis for hydrolysis reactions than its native counterpart. PMID:27129352

  19. 52 years of Kahl pilot nuclear power station. A special chapter in German technology history come to an end; 52 Jahre Versuchsatomkraftwerk Kahl. Ein besonderes Kapitel deutscher Technikgeschichte ist zu Ende

    Energy Technology Data Exchange (ETDEWEB)

    Hackel, Walter [VAK GmbH, Karlstein am Main (Germany)

    2011-06-15

    Since October 2010, the site of the former Kahl Experimental Nuclear Power Station (VAK) has been returned to the desired green-field condition. A special chapter in the German history of the use of nuclear power, which has played a major role in the technical and economic development of this energy resource, has come to an end after 52 years. When nuclear power pioneer Heinrich Mandel ordered VAK from AEG in 1958, the mood associated with using this source of energy was different from what it is now. Politicians of all parties were euphoric about the use of nuclear power, while power plant operators were more reluctant. Finally, also RWE was convinced and planned the construction of VAK as a joint venture with the Bayernwerk AG (now E.ON) utility in Grosswelzheim, Bavaria. The objective was clear: 'The order was placed so that the atomic power plant to be built could be used to accumulate experience in planning, operation, in capital cost and operating cost.' After a construction period of only 29 months, VAK was commissioned on November 13, 1960 as the first German nuclear power plant. The US-designed boiling water reactor had been designed for a power of 16,000 kilowatt. After synchronization with the power grid, the first electricity produced from nuclear power in Germany was fed into the interconnected grid system on June 17, 1961. In its 25 years of operation, VAK proved to be a valuable experimental facility. The plant was decommissioned on November 25, 1985 as planned. In order to maintain its experimental character also beyond the operating phase, the operators had agreed from the beginning to demolish VAK completely to green-field conditions immediately after the end of operations. This became official on May 17, 2010, when the plant was released from the scope of the Atomic Energy Act and was granted a permit for demolition under conventional building law. (orig.)

  20. Further observations on the utilization of adenosine triphosphate in rat mast cells during histamine release induced by the ionophore A23187

    DEFF Research Database (Denmark)

    Johansen, Torben

    1980-01-01

    1 The relation between A23187-induced histamine release and the energy metabolism of the rat mast cells has been studied. 2 Ethacrynic acid was used as an inhibitor of calcium-induced histamine release from mast cells primed with the ionophore A23187, and to study calcium-induced changes in the a......1 The relation between A23187-induced histamine release and the energy metabolism of the rat mast cells has been studied. 2 Ethacrynic acid was used as an inhibitor of calcium-induced histamine release from mast cells primed with the ionophore A23187, and to study calcium-induced changes...... demand of energy for exocytosis was estimated to be equivalent to 0.14 pmol of ATP pr 10(3) mast cells....

  1. Fampridine-PR (prolonged released 4-aminopyridine) is not effective in patients with inflammatory demyelination of the peripheral nervous system.

    Science.gov (United States)

    Leussink, Verena-Isabell; Stettner, Mark; Warnke, Clemens; Hartung, Hans-Peter

    2016-06-01

    Fampridine-PR is a voltage-gated potassium channel inhibitor potentially improving nerve conduction in demyelinated axons. Based on its established clinical efficacy in patients with demyelination in the central nervous system, we assessed if fampridine-PR is also effective in patients with inflammatory demyelination of the peripheral nerve. In this small open-label study, 10 patients with chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) were treated with fampridine-PR 10 mg BID for 28 days and assessed clinically as well as by nerve conduction studies. In this study, Fampridine-PR failed to improve CIDP based on clinical measures and nerve conduction studies. Our findings suggest that Fampridine-PR appears to be ineffective in demyelinating polyneuropathies. These observations may indicate a more complex mode of action beyond improving action potential conduction in demyelinated axons. PMID:26968589

  2. Seltenerdethenidhalogenide Ln2n+6(C2)n+4X2n+2: Darstellung, Struktur, Verwachsung und Verzwilligung

    OpenAIRE

    Mattausch, Hansjürgen; Kienle, Lorenz; Duppel, Viola; Hoch, Constantin; Simon, Arndt

    2009-01-01

    Abstract The compounds Ln10(C2)6X6 are formed by heating mixtures of LnX3, Ln und C in tantalum capsules under Ar atmosphere with the ratio LnX3:Ln:C = 2:4:6 for La10(C2)6Br6 at 1040 ?C, 30 d (yield ca. 20 %), Ce10(C2)6Br6 at 1200 ?C, 12 d, (80 %), Pr10(C2)6Br6 at 1140 ?C, 5 d, (20 %) and 2:8:12 for Gd10(C2)6Cl6 at 1170 ?C, 39 d, (100 %). They crystallize isotypical in a new structure type in C2/m with a = 22.809(5) ?, b = 3.9855(8) ?, c = 16.599(3) ?, ? = 123.32(3)? (La10(C2)6Br6)...

  3. AcEST: DK952525 [AcEST

    Lifescience Database Archive (English)

    Full Text Available /POZ domain-containing protein At5g4880... 155 1e-37 sp|Q682S0|RPT2_ARATH Root phototropism... protein 2 OS=Arabidopsis ... 137 3e-32 sp|Q9FMF5|RPT3_ARATH Root phototropism protein 3 OS=Arabid...opsis ... 136 7e-32 sp|Q5KS50|NPH3_ORYSJ Coleoptile phototropism protein 1 OS=Oryza ... 107 6e-23 sp|Q9S9Q9|... RV+ AM+ RG+R + +G ++V YA Sbjct: 235 RVMNAMKCRGVRPESIGASLVSYA 258 >sp|Q682S0|RPT2_ARATH Root phototropism pr...10 V+++M+ RG++ L AI+ Y Sbjct: 204 VVSSMKQRGVKPSSLASAIITY 225 >sp|Q9FMF5|RPT3_ARATH Root phototropism protein

  4. Entropy and energy spectra in low-Prandtl-number convection with rotation

    CERN Document Server

    Pharasi, Hirdesh K; Bhattacharjee, Jayanta K

    2014-01-01

    We present results for entropy and kinetic energy spectra computed from direct numerical simulations for low-Prandtl-number ($Pr 10^3$ for the rotation rates considered here. The entropy in the upper branch also shows scaling behavior with $k$, but the scaling exponent decreases with increasing $Ta$ for all $r$. The energy spectrum $E_v(k)$ is also found to scale with the wave number $k$ as $k^{-1.4\\pm 0.1}$ for $r > 10^3$. The scaling exponent for the energy spectrum and the lower branch of the entropy spectrum vary between $-1.7$ to $-2.4$ for lower values of $r$ ($< 10^3$). We also provide some simple arguments based on the variation of the Kolmogorov picture to support the results of simulations.

  5. R-CHOP方案初治B细胞非霍奇金淋巴瘤34例临床分析%Clinical analysis of initial CHOP regimen treatment for 34 cases of B cell non-Hodgkin lymphoma

    Institute of Scientific and Technical Information of China (English)

    王华; 姚红霞; 吴巨峰

    2010-01-01

    目的 观察R-CHOP方案治疗初治B细胞非霍奇金淋巴瘤(NHL)的疗效、安全性及不良反应.方法 回顾性分析2003年1月至2008年1月采用R-CHOP方案治疗34例初治B细胞NHL的疗效、安全性及不良反应.结果 CR21例(61.8%),PR 10例(29.4%),CR+PR 31例(91.2%),SD 1例,PD 2例.结论 R-CHOP方案治疗初治B细胞NHL疗效显著,效价比高,不良反应小,耐受好.

  6. Paromomycin Derived from Streptomyces sp. AG-P 1441 Induces Resistance against Two Major Pathogens of Chili Pepper.

    Science.gov (United States)

    Balaraju, Kotnala; Kim, Chang-Jin; Park, Dong-Jin; Nam, Ki-Woong; Zhang, Kecheng; Sang, Mee Kyung; Park, Kyungseok

    2016-09-28

    This is the first report that paromomycin, an antibiotic derived from Streptomyces sp. AG-P 1441 (AG-P 1441), controlled Phytophthora blight and soft rot diseases caused by Phytophthora capsici and Pectobacterium carotovorum, respectively, in chili pepper (Capsicum annum L.). Chili pepper plants treated with paromomycin by foliar spray or soil drenching 7 days prior to inoculation with P. capsici zoospores showed significant (p chili pepper. Furthermore, the treatment slightly promoted growth; this growth was supported by increased chlorophyll content in paromomycin-treated chili pepper plants. Additionally, paromomycin likely induced resistance as confirmed by the expression of pathogenesis-related (PR) genes: PR-1, β-1,3-glucanase, chitinase, PR-4, peroxidase, and PR-10, which enhanced plant defense against P. capsici in chili pepper. This finding indicates that AG-P 1441 plays a role in pathogen resistance upon the activation of defense genes, by secretion of the plant resistance elicitor, paromomycin. PMID:27291677

  7. Enlarging the toolbox for allergen epitope definition with an allergen-type model protein.

    Directory of Open Access Journals (Sweden)

    Hanna Berkner

    Full Text Available Birch pollen-allergic subjects produce polyclonal cross-reactive IgE antibodies that mediate pollen-associated food allergies. The major allergen Bet v 1 and its homologs in plant foods bind IgE in their native protein conformation. Information on location, number and clinical relevance of IgE epitopes is limited. We addressed the use of an allergen-related protein model to identify amino acids critical for IgE binding of PR-10 allergens.Norcoclaurine synthase (NCS from meadow rue is structurally homologous to Bet v 1 but does not bind Bet v 1-reactive IgE. NCS was used as the template for epitope grafting. NCS variants were tested with sera from 70 birch pollen allergic subjects and with monoclonal antibody BV16 reported to compete with IgE binding to Bet v 1.We generated an NCS variant (Δ29NCSN57/I58E/D60N/V63P/D68K harboring an IgE epitope of Bet v 1. Bet v 1-type protein folding of the NCS variant was evaluated by 1H-15N-HSQC NMR spectroscopy. BV16 bound the NCS variant and 71% (50/70 sera of our study population showed significant IgE binding. We observed IgE and BV16 cross-reactivity to the epitope presented by the NCS variant in a subgroup of Bet v 1-related allergens. Moreover BV16 blocked IgE binding to the NCS variant. Antibody cross-reactivity depended on a defined orientation of amino acids within the Bet v 1-type conformation.Our system allows the evaluation of patient-specific epitope profiles and will facilitate both the identification of clinically relevant epitopes as biomarkers and the monitoring of therapeutic outcomes to improve diagnosis, prognosis, and therapy of allergies caused by PR-10 proteins.

  8. Overexpression of GmERF5, a new member of the soybean EAR motif-containing ERF transcription factor, enhances resistance to Phytophthora sojae in soybean.

    Science.gov (United States)

    Dong, Lidong; Cheng, Yingxin; Wu, Junjiang; Cheng, Qun; Li, Wenbin; Fan, Sujie; Jiang, Liangyu; Xu, Zhaolong; Kong, Fanjiang; Zhang, Dayong; Xu, Pengfei; Zhang, Shuzhen

    2015-05-01

    Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.], caused by Phytophthora sojae Kaufmann and Gerdemann, is a destructive disease throughout the soybean planting regions in the world. Here, we report insights into the function and underlying mechanisms of a novel ethylene response factor (ERF) in soybean, namely GmERF5, in host responses to P. sojae. GmERF5-overexpressing transgenic soybean exhibited significantly enhanced resistance to P. sojae and positively regulated the expression of the PR10, PR1-1, and PR10-1 genes. Sequence analysis suggested that GmERF5 contains an AP2/ERF domain of 58 aa and a conserved ERF-associated amphiphilic repression (EAR) motif in its C-terminal region. Following stress treatments, GmERF5 was significantly induced by P. sojae, ethylene (ET), abscisic acid (ABA), and salicylic acid (SA). The activity of the GmERF5 promoter (GmERF5P) was upregulated in tobacco leaves with ET, ABA, Phytophthora nicotianae, salt, and drought treatments, suggesting that GmERF5 could be involved not only in the induced defence response but also in the ABA-mediated pathway of salt and drought tolerance. GmERF5 could bind to the GCC-box element and act as a repressor of gene transcription. It was targeted to the nucleus when transiently expressed in Arabidopsis protoplasts. GmERF5 interacted with a basic helix-loop-helix transcription factor (GmbHLH) and eukaryotic translation initiation factor (GmEIF) both in yeast cells and in planta. To the best of our knowledge, GmERF5 is the first soybean EAR motif-containing ERF transcription factor demonstrated to be involved in the response to pathogen infection. PMID:25779701

  9. Pr2Fe14B/α-Fe nanocomposites for thermal applications

    International Nuclear Information System (INIS)

    In this work, PrxFe94-xB6 (x = 6, 8, 10 and 12) nanostructured powders were prepared by a combination of hydrogenation, disproportionation, desorption and recombination (HDDR) process with high energy milling applied to the mixture of an as-cast alloy (Pr14Fe80B6) and α-Fe. The produced nanoparticles showed magnetic properties comparable to those reported in hyperthermia studies. The optimal time to obtain the magnetic nanoparticles is 5 hours (at 900 rpm). It was verified that longer milling times cause an increase in carbon percentage on the particles. The carbon is derived from oleic acid added as a surfactant in the milling step. The nanocomposites exhibit coercive force ranging from 80 Oe (6.5 kAm-1) to 170 Oe (13.5 kAm-1) and magnetic moments in the range of 81 129 Am2kg-1. From the X-ray diffraction analyses, only two phases were found in all samples: α-Fe and the magnetic phase Pr2Fe14B. Individual nanoparticles with diameter of about 20 nm were verified. The samples studied presented heating when exposed to an alternating magnetic field (f = 222 kHz e Hmax ∼3.7 kAm-1) comparable to reported in literature. Temperature variations (ΔT) of the powders were: 51 K for Pr6Fe88B6, 41 K for Pr8Fe86B6, 38 K for Pr10Fe84B6 and T = 34 K for Pr12Fe82B6. The specific absorption rates (SARs) of the powders were 201 Wkg-1 for Pr6Fe88B6 composition, 158 Wkg-1 on the composition Pr8Fe86B6, and 114 Wkg-1 for Pr10Fe84B6 and Pr12Fe82B6 compositions. (author)

  10. Alcohol reward is increased after Roux-en-Y gastric bypass in dietary obese rats with differential effects following ghrelin antagonism.

    Directory of Open Access Journals (Sweden)

    Andras Hajnal

    Full Text Available Roux-en-Y gastric bypass (RYGB is one of the most successful treatments for severe obesity and associated comorbidities. One potential adverse outcome, however, is increased risk for alcohol use. As such, we tested whether RYGB alters motivation to self-administer alcohol in outbred dietary obese rats, and investigated the involvement of the ghrelin system as a potential underlying mechanism. High fat (60%kcal from fat diet-induced obese, non-diabetic male Sprague Dawley rats underwent RYGB (n = 9 or sham operation (Sham, n = 9 and were tested 4 months after surgery on a progressive ratio-10 (PR10 schedule of reinforcement operant task for 2, 4, and 8% ethanol. In addition, the effects of the ghrelin-1a-receptor antagonist D-[Lys3]-GHRP-6 (50, 100 nmol/kg, IP were tested on PR10 responding for 4% ethanol. Compared to Sham, RYGB rats made significantly more active spout responses to earn reward, more consummatory licks on the ethanol spout, and achieved higher breakpoints. Pretreatment with a single peripheral injection of D-[Lys3]-GHRP-6 at either dose was ineffective in altering appetitive or consummatory responses to 4% ethanol in the Sham group. In contrast, RYGB rats demonstrated reduced operant performance to earn alcohol reward on the test day and reduced consummatory responses for two subsequent days following the drug. Sensitivity to threshold doses of D-[LYS3]-GHRP-6 suggests that an augmented ghrelin system may contribute to increased alcohol reward in RYGB. Further research is warranted to confirm applicability of these findings to humans and to explore ghrelin-receptor targets for treatment of alcohol-related disorders in RYGB patients.

  11. 化疗对肺癌疼痛镇痛作用的临床评价%Clinical evaluation of analgesic efficacy of chemotherapy in 63 patients with lung cancer pain

    Institute of Scientific and Technical Information of China (English)

    丁永为

    2005-01-01

    目的评价化疗对肺癌疼痛的镇痛作用并探讨肺癌疼痛的治疗策略.方法 63例肺癌中度疼痛40例,重度疼痛23例.16例小细胞肺癌以CEP(CTX,Vp-16,DDP)或VMF-CP(Vp-16,MTX,5-Fu,CTX,DDP)方案化疗,47例非小细胞肺癌以NP(NVB,DDP)或CAP(CTX,ADM,DDP)方案化疗,并评价镇痛作用.结果疼痛完全缓解(CR)34例(54.0%),部分缓解(PR)27例(42.9%),无效2例,总疼痛缓解率96.8%.16例小细胞未分化癌疼痛缓解率100%,CR 11例(68.8%),PR 5例(31.2%);22例差分化癌疼痛缓解率100%,CR 12例(54.5%),PR 10例(45.5%);17例高分化鳞、腺癌疼痛缓解率94.1%,CR 6例(35.3%),PR 10例(58.8%),无效1例,不同分化程度肺癌疼痛CR率差异有显著性(χ2=4.0166,P0.05).结论化疗对缓解肺癌疼痛疗效显著,可作为肺癌疼痛的重要治疗措施.分化程度、疼痛部位和肿瘤客观疗效显著影响化疗镇痛效果.

  12. Characterization of Withania somnifera leaf transcriptome and expression analysis of pathogenesis-related genes during salicylic acid signaling.

    Science.gov (United States)

    Ghosh Dasgupta, Modhumita; George, Blessan Santhosh; Bhatia, Anil; Sidhu, Om Prakash

    2014-01-01

    Withania somnifera (L.) Dunal is a valued medicinal plant with pharmaceutical applications. The present study was undertaken to analyze the salicylic acid induced leaf transcriptome of W. somnifera. A total of 45.6 million reads were generated and the de novo assembly yielded 73,523 transcript contig with average transcript contig length of 1620 bp. A total of 71,062 transcripts were annotated and 53,424 of them were assigned GO terms. Mapping of transcript contigs to biological pathways revealed presence of 182 pathways. Seventeen genes representing 12 pathogenesis-related (PR) families were mined from the transcriptome data and their pattern of expression post 17 and 36 hours of salicylic acid treatment was documented. The analysis revealed significant up-regulation of all families of PR genes by 36 hours post treatment except WsPR10. The relative fold expression of transcripts ranged from 1 fold to 6,532 fold. The two families of peroxidases including the lignin-forming anionic peroxidase (WsL-PRX) and suberization-associated anionic peroxidase (WsS-PRX) recorded maximum expression of 377 fold and 6532 fold respectively, while the expression of WsPR10 was down-regulated by 14 fold. Additionally, the most stable reference gene for normalization of qRT-PCR data was also identified. The effect of SA on the accumulation of major secondary metabolites of W. somnifera including withanoside V, withaferin A and withanolide A was also analyzed and an increase in content of all the three metabolites were detected. This is the first report on expression patterns of PR genes during salicylic acid signaling in W. somnifera.

  13. Sisseminekuks : [luuletused] / Anna Haava ; [Impressioonid]: Viiu Härm

    Index Scriptorium Estoniae

    Haava, Anna, pseud., 1864-1957

    2004-01-01

    Sisu: Sisseminekuks ; Kartus ; Ööbikule ; Käokene, kuldalindu ; Mis sa tahad, kevade? ; Ei tule uni ; Hoia ennast; Tuksuv süda ; Sina oled kelm ; Hinge hellal' igatsusel' ; Kõik kallile ; Soov ; Unes nägin ; Võin ; Sinu silmad ; Sinu silmist pilk ; Kallis, sul on kuldne süda ; Sa kõige armsam mulle ; Oh võiksin ; Lill ; Nõmmelill ; Meie Mihkel ; Koidulale ; Ehitage rahukojad ; Emakeel ; Isamaja ; Väsinud ; Oh kuhu ma lähen? ; Ööbiku surm ; Ei saa mitte vaiki olla ; Ma olen näinud ; Südameta ; Sellepärast ; See oli ilusal õiekuul; Esimese lume ajal ; Mina vaat'sin muru pääle ; Ikka kurjalt sa mind vaatad ; Oh ma isegi ei tea ; Mis on luule paljalt sõnas? ; Oh oleksin ära surnud ; Lee ääres ; Sinu nimi ; Hoia vahest aken lahti ; Jumalaga jättes vaat'sid ; Ma laulan ; Ei oma õnne peita ; Küll oli ilus mu õieke ; Oh ära sa küsi ; Ei tule luule tuulest ; Köögis seisab ; Järv leegib eha paistel ; Sinu vanaema ; Nii ta on ; Tead küll, mu kuldkallis ; Ma lähen üle nõmme ; Lenda, lepatriinuke ; Vesi voolab, vesi kohab ; Üks ainus kord ; Oh kevade, oh õie-aeg ; Suur kontsert ; Kuupaistel ; Mets kohiseb ; Lind läks magama ; Kui sa tuled, too mull' lilli ; Sinilillekesed ; Ei ole sina minu ingel ; Ei mina õnne usu ; Viimast laulu ei saa laulda ; Kõige ilusam luule ; Üks on minul püham koda! ; Sa oled suurem kui su saatus ; Mu kodumaa, kus oled sa? ; Eest ära! ; Kui kandlekeeled katkevad ; Ma kõnnin koiduvalgel ; Mu mured kõik matsin ma maha ; Kui ma sulle armas olen ; Kas tahad tulla minuga? ; Sa vaata ette, süda! ; Truus sõpruses ma tahan ; Su silmade sügavuses ; Siis mine! ; See oli siis ; Mu süda usub ; Sind teretame, kuldapäike ; Mind jumalaga jätsid ; Sääl kord kasvab kaunis kodu ; Me oleme põhjamaa lapsed ; Hilised lilled ; Sa küsid, kus mu kodu ; külm ; Sina vaikiv, sügav öö ; Ju õitsvad kõik hellerheinad ; Öösünges kohab ; Kui saaks mullegi kord kodu ; On püha mulle mu kodumaa muld ; Oma hind vaid vaba

  14. Identifying Patient-Specific Epstein-Barr Nuclear Antigen-1 Genetic Variation and Potential Autoreactive Targets Relevant to Multiple Sclerosis Pathogenesis.

    Directory of Open Access Journals (Sweden)

    Monika Tschochner

    Full Text Available Epstein-Barr virus (EBV infection represents a major environmental risk factor for multiple sclerosis (MS, with evidence of selective expansion of Epstein-Barr Nuclear Antigen-1 (EBNA1-specific CD4+ T cells that cross-recognize MS-associated myelin antigens in MS patients. HLA-DRB1*15-restricted antigen presentation also appears to determine susceptibility given its role as a dominant risk allele. In this study, we have utilised standard and next-generation sequencing techniques to investigate EBNA-1 sequence variation and its relationship to HLA-DR15 binding affinity, as well as examining potential cross-reactive immune targets within the central nervous system proteome.Sanger sequencing was performed on DNA isolated from peripheral blood samples from 73 Western Australian MS cases, without requirement for primary culture, with additional FLX 454 Roche sequencing in 23 samples to identify low-frequency variants. Patient-derived viral sequences were used to predict HLA-DRB1*1501 epitopes (NetMHCII, NetMHCIIpan and candidates were evaluated for cross recognition with human brain proteins.EBNA-1 sequence variation was limited, with no evidence of multiple viral strains and only low levels of variation identified by FLX technology (8.3% nucleotide positions at a 1% cut-off. In silico epitope mapping revealed two known HLA-DRB1*1501-restricted epitopes ('AEG': aa 481-496 and 'MVF': aa 562-577, and two putative epitopes between positions 502-543. We identified potential cross-reactive targets involving a number of major myelin antigens including experimentally confirmed HLA-DRB1*15-restricted epitopes as well as novel candidate antigens within myelin and paranodal assembly proteins that may be relevant to MS pathogenesis.This study demonstrates the feasibility of obtaining autologous EBNA-1 sequences directly from buffy coat samples, and confirms divergence of these sequences from standard laboratory strains. This approach has identified a number of

  15. Ginsenoside Rg1 prevents cognitive impairment and hippocampus senescence in a rat model of D-galactose-induced aging.

    Directory of Open Access Journals (Sweden)

    Jiahong Zhu

    Full Text Available Neurogenesis continues throughout the lifetime in the hippocampus, while the rate declines with brain aging. It has been hypothesized that reduced neurogenesis may contribute to age-related cognitive impairment. Ginsenoside Rg1 is an active ingredient of Panax ginseng in traditional Chinese medicine, which exerts anti-oxidative and anti-aging effects. This study explores the neuroprotective effect of ginsenoside Rg1 on the hippocampus of the D-gal (D-galactose induced aging rat model. Sub-acute aging was induced in male SD rats by subcutaneous injection of D-gal (120 mg/kg·d for 42 days, and the rats were treated with ginsenoside Rg1 (20 mg/kg·d, intraperitoneally or normal saline for 28 days after 14 days of D-gal injection. In another group, normal male SD rats were treated with ginsenoside Rg1 alone (20 mg/kg·d, intraperitoneally for 28 days. It showed that administration of ginsenoside Rg1 significantly attenuated all the D-gal-induced changes in the hippocampus, including cognitive capacity, senescence-related markers and hippocampal neurogenesis, compared with the D-gal-treated rats. Further investigation showed that ginsenoside Rg1 protected NSCs/NPCs (neural stem cells/progenitor cells shown by increased level of SOX-2 expression; reduced astrocytes activation shown by decrease level of Aeg-1 expression; increased the hippocampal cell proliferation; enhanced the activity of the antioxidant enzymes GSH-Px (glutathione peroxidase and SOD (Superoxide Dismutase; decreased the levels of IL-1β, IL-6 and TNF-α, which are the proinflammatory cytokines; increased the telomere lengths and telomerase activity; and down-regulated the mRNA expression of cellular senescence associated genes p53, p21Cip1/Waf1 and p19Arf in the hippocampus of aged rats. Our data provides evidence that ginsenoside Rg1 can improve cognitive ability, protect NSCs/NPCs and promote neurogenesis by enhancing the antioxidant and anti-inflammatory capacity in the

  16. Dielectric breakdown of cell membranes.

    Science.gov (United States)

    Zimmermann, U; Pilwat, G; Riemann, F

    1974-11-01

    With human and bovine red blood cells and Escherichia coli B, dielectric breakdown of cell membranes could be demonstrated using a Coulter Counter (AEG-Telefunken, Ulm, West Germany) with a hydrodynamic focusing orifice. In making measurements of the size distributions of red blood cells and bacteria versus increasing electric field strength and plotting the pulse heights versus the electric field strength, a sharp bend in the otherwise linear curve is observed due to the dielectric breakdown of the membranes. Solution of Laplace's equation for the electric field generated yields a value of about 1.6 V for the membrane potential at which dielectric breakdown occurs with modal volumes of red blood cells and bacteria. The same value is also calculated for red blood cells by applying the capacitor spring model of Crowley (1973. Biophys. J. 13:711). The corresponding electric field strength generated in the membrane at breakdown is of the order of 4 . 10(6) V/cm and, therefore, comparable with the breakdown voltages for bilayers of most oils. The critical detector voltage for breakdown depends on the volume of the cells. The volume-dependence predicted by Laplace theory with the assumption that the potential generated across the membrane is independent of volume, could be verified experimentally. Due to dielectric breakdown the red blood cells lose hemoglobin completely. This phenomenon was used to study dielectric breakdown of red blood cells in a homogeneous electric field between two flat platinum electrodes. The electric field was applied by discharging a high voltage storage capacitor via a spark gap. The calculated value of the membrane potential generated to produce dielectric breakdown in the homogeneous field is of the same order as found by means of the Coulter Counter. This indicates that mechanical rupture of the red blood cells by the hydrodynamic forces in the orifice of the Coulter Counter could also be excluded as a hemolysing mechanism. The detector

  17. International Association for Promoting Geoethics (IAPG): an update on activities

    Science.gov (United States)

    Di Capua, Giuseppe; Bobrowsky, Peter; Kieffer, Susan; Peppoloni, Silvia; Tinti, Stefano

    2016-04-01

    The International Association for Promoting Geoethics (IAPG: http://www.geoethics.org) was founded on August 2012 to unite global geoscientists to raise the awareness of the scientific community regarding the importance of the ethical, social and cultural implications of geoscience research, education, and practice. IAPG is an international, multidisciplinary and scientific platform for discussion on ethical problems and dilemmas in Earth Sciences, promoting geoethical themes through scientific publications and conferences, strengthening the research base on geoethics, and focusing on case-studies as models for the development of effective and operative strategies. IAPG is legally recognized as a not-for-profit organization. It is a non-governmental, non-political, non-party institution, at all times free from racial, gender, religious or national prejudices. Its network continues to grow with more than 900 members in 103 countries, including 20 national sections. IAPG operates exclusively through donations and personal funds of its members. The results achieved since inception have been recognized by numerous international organizations. In particular, IAPG has obtained the status of affiliated organization by the International Union of Geological Sciences (IUGS), American Geosciences Institute (AGI), Geological Society of America (GSA), and the Geological Society of London (GSL). IAPG has enlarged its official relationships also through agreements on collaboration with other organizations, such as the American Geophysical Union (AGU), EuroGeoSurveys (EGS), European Federation of Geologists (EFG), Association of Environmental & Engineering Geologists (AEG), International Geoscience Education Organisation (IGEO), African Association of Women in Geosciences (AAWG), and others. IAPG considers publications as an indispensable activity to strengthen geoethics from a scientific point of view, so members are active in the publication of articles and editing of books on

  18. 降低社会物流总费用占GDP比重的对策研究--基于多元回归模型的实证分析%Countermeasures to Reduce the Total Social Logistics Costs Accounted for the Proportion of GDP---An Empirical Analysis Based on Multiple Regression Model

    Institute of Scientific and Technical Information of China (English)

    汪文生; 张娟

    2016-01-01

    一个国家或地区的物流发展水平与当地的社会物流总费用占GDP的比重息息相关,理论上,这一比例不宜过高,如何采取有效措施来降低该比例已成为当务之急。本文基于多元回归模型,运用Eviews软件对可能影响该比例的四大因素进行了回归分析及ADF、AEG协整检验,研究结果表明:产业结构对社会物流总费用占GDP比重的影响最为显著;物流行业就业人数的影响作用次之;而物流基础设施投资、经济发展水平对该比例的影响并不显著。在此基础上,分析了回归结果的经济原因并联系实际情况给出了相应的可行性政策建议。%The logistics development level of a country or region is closely related to the local total social logistics costs accounted for the proportion of GDP . In theory , the ratio should't be too high . And how to take effective measures to reduce the proportion has become a top priority . The paper conducts the multiple regression model and make the regression analysis and ADF , AEG cointegration test for four possible factors by using Eviews software . The research results show that , the industrial structure is the most significant factor ;the em-ployment in the logistics industry takes the second place ;the logistics infrastructure investment and the level of economic development are not the obvious influence factors . On this basis , the economic reasons of the regression results are analyzed , and the feasible policy sug-gestions are given with practice .

  19. Lasteaia ja lõbumaja vahel : eesti proosa 2008 / Vaapo Vaher

    Index Scriptorium Estoniae

    Vaher, Vaapo, 1945-

    2009-01-01

    Ülevaade 2008. aasta eesti proosast. Pikemalt käsitletakse järgmisi autoreid ja nende teoseid: Mihkel Raud "Musta pori näkku", Olev Remsu "Musketäride muundumised", Rein Põder "Juba olnud", Juhan Nurme "Ligimene", Jaan Tangsoo "Méduse'i parv", Esta Aksli "Tare poole tõttaja", Mari Saat "Lasnamäe lunastaja", Peeter Morgen "Sõnum", Mait Raun "Dr. Mööbi seiklused Inglismaal", Andrei Hvostov "Võõrad lood" ja "Projektijuht Posse", Chaneldior "Kontrolli alt väljas", Vahur Afanasjev "Kosmos", Egon Ilisson "Isased", Jaak Leer "Kaks kabanossi ja Maaja", Kärt Hellerma "Sinine missa", Elo Viiding "Püha Maama", Toomas Raudam "Miks ma ei taha olla kirjanik?", Mart Kivastik "Kurb raamat", Jüri Tuulik "Räim, pisike kena kala", Tarmo Teder "12 jõulujuttu", Dagmar Lamp (koostaja) "Tule, ma jutustan sulle loo", Eia Uus "Kahe näoga jumal", Barthol Lo Mejor "Popdada 2007-2008", Anti Saar "Nemad kaks", Ketlin Priilinn "Liiseli võti" ja "Vaim", Aita Kivi "Lähedal", Marje Ernits "Hoia mu kätt", Kati Murutar "Naisena sündinud : kakskümmend aastat hiljem", Tuule Lind "Pööratud tuul", Siiri Laidla "Lihtne lugu, ehk, Senta suvepuhkus", Kätlin Padesaar "Veel üks paradiis", Aimée Beekman "Proovielu", Lehte Hainslau "Kolmkõla, ehk, 3 kõla", Ira Lember "Ööviiulid", Tamur Kusnets "Hundipäikese aeg : metsiku jahi algus", Kaupo Pähkel "Ehatähe rüütel", Arvo Valton "Ülemiste vanake", Tiit Aleksejev "Palveränd", Ants Eret "Afgaanide vang", Mario Merirand "Afganistani päevik", Leo Kunnas "Gort Ashryn", Rein Raud "Vend", Peeter Sauter "Beibi bluu", Marion Andra "Pimend", Kristjan Sander "13 talvist hetke", Helga Nõu "Peaaegu geenius, ehk, Schrödingeri kassi otsimas", Tiit Sepa "Eefa hüüdnimega Siug", Kaarel ja Klaara Kivi "Varandus seinal", Merike Jürjo "Mälestusteta maja", Rein Vahisalu "Viies kabinet", Aarne Biin "Mees otsib naist", Erik Tohvri "Ümberasujad" ja "Sulasest sai peremees", Arnold Laugus "Elav maa", Jüri v. Grauberg "Selguse poole", J

  20. Lasteaia ja lõbumaja vahel : Eesti proosa 2008 / Vaapo Vaher

    Index Scriptorium Estoniae

    Vaher, Vaapo, 1945-

    2012-01-01

    Ülevaade 2008. aasta eesti proosast. Pikemalt käsitletakse järgmisi autoreid ja nende teoseid: Mihkel Raud "Musta pori näkku", Olev Remsu "Musketäride muundumised", Rein Põder "Juba olnud", Juhan Nurme "Ligimene", Jaan Tangsoo "Méduse'i parv", Esta Aksli "Tare poole tõttaja", Mari Saat "Lasnamäe lunastaja", Peeter Morgen "Sõnum", Mait Raun "Dr. Mööbi seiklused Inglismaal", Andrei Hvostov "Võõrad lood" ja "Projektijuht Posse", Chaneldior "Kontrolli alt väljas", Vahur Afanasjev "Kosmos", Egon Ilisson "Isased", Jaak Leer "Kaks kabanossi ja Maaja", Kärt Hellerma "Sinine missa", Elo Viiding "Püha Maama", Toomas Raudam "Miks ma ei taha olla kirjanik?", Mart Kivastik "Kurb raamat", Jüri Tuulik "Räim, pisike kena kala", Tarmo Teder "12 jõulujuttu", Dagmar Lamp (koostaja) "Tule, ma jutustan sulle loo", Eia Uus "Kahe näoga jumal", Barthol Lo Mejor "Popdada 2007-2008", Anti Saar "Nemad kaks", Ketlin Priilinn "Liiseli võti" ja "Vaim", Aita Kivi "Lähedal", Marje Ernits "Hoia mu kätt", Kati Murutar "Naisena sündinud : kakskümmend aastat hiljem", Tuule Lind "Pööratud tuul", Siiri Laidla "Lihtne lugu, ehk, Senta suvepuhkus", Kätlin Padesaar "Veel üks paradiis", Aimée Beekman "Proovielu", Lehte Hainslau "Kolmkõla, ehk, 3 kõla", Ira Lember "Ööviiulid", Tamur Kusnets "Hundipäikese aeg : metsiku jahi algus", Kaupo Pähkel "Ehatähe rüütel", Arvo Valton "Ülemiste vanake", Tiit Aleksejev "Palveränd", Ants Eret "Afgaanide vang", Mario Merirand "Afganistani päevik", Leo Kunnas "Gort Ashryn", Rein Raud "Vend", Peeter Sauter "Beibi bluu", Marion Andra "Pimend", Kristjan Sander "13 talvist hetke", Helga Nõu "Peaaegu geenius, ehk, Schrödingeri kassi otsimas", Tiit Sepa "Eefa hüüdnimega Siug", Kaarel ja Klaara Kivi "Varandus seinal", Merike Jürjo "Mälestusteta maja", Rein Vahisalu "Viies kabinet", Aarne Biin "Mees otsib naist", Erik Tohvri "Ümberasujad" ja "Sulasest sai peremees", Arnold Laugus "Elav maa", Jüri v. Grauberg "Selguse poole", J

  1. Recém-nascido pequeno para a idade gestacional: repercussão nas habilidades motoras finas Pequeño para la edad gestacional: repercusión en las habilidades motoras finas Small-for-gestational-age newborn infant: repercussion on fine motor skills

    Directory of Open Access Journals (Sweden)

    Amabile Vessoni Arias

    2011-03-01

    Full Text Available OBJETIVO: Comparar as habilidades motoras finas de lactentes nascidos a termo pequenos para a idade gestacional (PIG com as habilidades dos nascidos adequados para a idade gestacional (AIG no terceiro mês de vida. MÉTODOS: Realizou-se um estudo observacional de corte transversal. Avaliaram-se 67 lactentes (21 PIG e 46 AIG no terceiro mês de vida. Portadores de síndromes genéticas, malformações congênitas, infecções congênitas e aqueles que necessitaram de unidade de terapia intensiva neonatal foram excluídos. As Escalas Bayley II de Desenvolvimento Infantil foram utilizadas, com ênfase nos itens que avaliam as habilidades motoras finas. RESULTADOS: Não se observaram diferenças entre os grupos na escala motora (p=0,21 e mental (p=0,45 no terceiro mês de vida. No item "Alcança o Aro Suspenso", houve diferença significativa (teste Exato de Fisher; p=0,02, demonstrando maior frequência de execução para o grupo PIG no terceiro mês de vida. CONCLUSÕES: Supõe-se que a diferença encontrada no item "Alcança o Aro Suspenso" possa ser atribuída à grande ocorrência de movimentos dos braços observada no grupo PIG e não a uma condição melhor de desenvolvimento desse grupo.OBJETIVO: Comparar las habilidades motoras finas de lactantes nacidos a término pequeños para la edad gestacional (PEG con adecuados para la edad gestacional (AEG en el 3er mes de vida. MÉTODOS: Se realizó un estudio observacional de corte transversal. Se evaluaron a 67 lactantes (21 PEG y 46 AEG en el 3er mes de vida. Síndromes genéticos, malformaciones congénitas, infecciones congénitas y aquellos que necesitaron de unidad de terapia intensiva neonatal fueron excluidos. Las Escalas Bayley II y de Desarrollo Infantil fueron utilizadas, con énfasis en los ítems que evalúan las habilidades motoras finas. RESULTADOS: No se observaron diferencias entre los grupos en la escala motora (p=0,21 y mental (p=0,45 en el 3er mes de vida. En el ítem "alcanza

  2. Tumor response and clinical outcome in metastatic gastrointestinal stromal tumors under sunitinib therapy: Comparison of RECIST, Choi and volumetric criteria

    Energy Technology Data Exchange (ETDEWEB)

    Schramm, N., E-mail: Nicolai.schramm@med.uni-muenchen.de [Institute for Clinical Radiology, Ludwig-Maximilians-University Hospital Munich, Marchioninistrasse 15, 81377 Munich (Germany); Englhart, E., E-mail: Elisabeth.Englhart@gmx.de [Institute for Clinical Radiology, Ludwig-Maximilians-University Hospital Munich, Marchioninistrasse 15, 81377 Munich (Germany); Schlemmer, M., E-mail: Marcus.Schlemmer@med.uni-muenchen.de [Department of Medicine III, Ludwig-Maximilians-University Hospital Munich, Marchioninistrasse 15, 81377 Munich (Germany); Hittinger, M., E-mail: Markus.Hittinger@uksh.de [Institute for Clinical Radiology, Ludwig-Maximilians-University Hospital Munich, Marchioninistrasse 15, 81377 Munich (Germany); Übleis, C., E-mail: Christopher.Uebleis@med.uni-muenchen.de [Department of Nuclear Medicine, Ludwig-Maximilians-University Hospital Munich, Marchioninistrasse 15, 81377 Munich (Germany); Becker, C.R., E-mail: Christoph.becker@med.uni-muenchen.de [Institute for Clinical Radiology, Ludwig-Maximilians-University Hospital Munich, Marchioninistrasse 15, 81377 Munich (Germany); Reiser, M.F., E-mail: Maximilian.Reiser@med.uni-muenchen.de [Institute for Clinical Radiology, Ludwig-Maximilians-University Hospital Munich, Marchioninistrasse 15, 81377 Munich (Germany); Berger, F., E-mail: Frank.Berger@med.uni-muenchen.de [Institute for Clinical Radiology, Ludwig-Maximilians-University Hospital Munich, Marchioninistrasse 15, 81377 Munich (Germany)

    2013-06-15

    Purpose: Purpose of the study was to compare radiological treatment response according to RECIST, Choi and volumetry in GIST-patients under 2nd-line-sunitinib-therapy and to correlate the results of treatment response assessment with disease-specific survival (DSS). Patients and methods: 20 patients (mean: 60.7 years; 12 male/8 female) with histologically proven GIST underwent baseline-CT of the abdomen under imatinib and follow-up-CTs 3 months and 1 year after change to sunitinib. 68 target lesions (50 hepatic, 18 extrahepatic) were investigated. Therapy response (partial response (PR), stable disease (SD), progressive disease (PD)) was evaluated according to RECIST, Choi and volumetric criteria. Response according to the different assessment systems was compared and correlated to the DSS of the patients utilizing Kaplan–Meier statistics. Results: The mean DSS (in months) of the response groups 3 months after therapy change was: RECIST: PR (0/20); SD (17/20): 30.4 (months); PD (3/20) 11.6. Choi: PR (10/20) 28.6; SD (8/20) 28.1; PD (2/20) 13.5. Volumetry: PR (4/20) 29.6; SD (11/20) 29.7; PD (5/20) 17.2. Response groups after 1 year of sunitinib showed the following mean DSS: RECIST: PR (3/20) 33.6; SD (9/20) 29.7; PD (8/20) 20.3. Choi: PR (10/20) 21.5; SD (4/20) 42.9; PD (6/20) 23.9. Volumetry: PR (6/20) 27.3; SD (5/20) 38.5; PD (9/20) 19.3. Conclusion: One year after modification of therapy, only partial response according to RECIST indicated favorable survival in patients with GIST. The value of alternate response assessment strategies like Choi criteria for prediction of survival in molecular therapy still has to be demonstrated.

  3. The Short Series of the Oxygen-Poor Lanthanide Oxide Selenides M10OSe14 with M = La–Nd

    Directory of Open Access Journals (Sweden)

    Frank A. Weber

    2012-08-01

    Full Text Available Single crystals and phase pure samples of oxygen-poor ternary lanthanide oxide selenides with the composition M10OSe14 (M = La–Nd; tetragonal, I41/acd; a = 1592.0–1559.8 pm, c = 2106.5–2062.9 pm could be obtained by reacting the corresponding metals, selenium and selenium dioxide as oxygen source. Their crystal structures are isotypic with Pr10OS14 and thus contain isolated [OM4]10+ tetrahedra (d(O2––M3+ = 243–248 pm embedded in a complex anionic {[M6Se14]10–} lanthanide selenide matrix (d(M3+–Se2– = 288–358 pm. All three crystallographically independent M3+ cations exhibit eight contacts to chalcogenide anions (O2– and/or Se2– resulting in the formation of bicapped trigonal prismatic coordination polyhedra. The optical band gaps of the oxide selenides M10OSe14 amount to values between 1.89 and 2.04 eV indicating wide band-gap semiconductors.

  4. Tissue-specific expression of Arabidopsis NPR1 gene in rice for sheath blight resistance without compromising phenotypic cost.

    Science.gov (United States)

    Molla, Kutubuddin Ali; Karmakar, Subhasis; Chanda, Palas Kumar; Sarkar, Sailendra Nath; Datta, Swapan Kumar; Datta, Karabi

    2016-09-01

    Rice sheath blight disease, caused by the fungus Rhizoctonia solani, is considered the second most important disease of rice after blast. NPR1 (non expressor of PR1) is the central regulator of systemic acquired resistance (SAR) conferring broad spectrum resistance to various pathogens. Previous reports have indicated that constitutive expression of the Arabidopsis thaliana NPR1 (AtNPR1) gene results in disease resistance in rice but has a negative impact on growth and agronomic traits. Here, we report that green tissue-specific expression of AtNPR1 in rice confers resistance to the sheath blight pathogen, with no concomitant abnormalities in plant growth and yield parameters. Elevated levels of NPR1 activated the defence pathway in the transgenic plants by inducing expression of endogenous genes such as PR1b, RC24, and PR10A. Enhanced sheath blight resistance of the transgenic plants was evaluated using three different bioassay systems. A partially isolated toxin from R. solani was used in the bioassays to measure the resistance level. Studies of the phenotype and yield showed that the transgenic plants did not exhibit any kind of phenotypic imbalances. Our results demonstrate that green tissue-specific expression of AtNPR1 is an effective strategy for controlling the sheath blight pathogen. The present work in rice can be extended to other crop plants severely damaged by the pathogen. PMID:27457988

  5. Molecular and biochemical classification of plant-derived food allergens.

    Science.gov (United States)

    Breiteneder, H; Ebner, C

    2000-07-01

    Molecular biology and biochemical techniques have significantly advanced the knowledge of allergens derived from plant foods. Surprisingly, many of the known plant food allergens are homologous to pathogenesis-related proteins (PRs), proteins that are induced by pathogens, wounding, or certain environmental stresses. PRs have been classified into 14 families. Examples of allergens homologous to PRs include chitinases (PR-3 family) from avocado, banana, and chestnut; antifungal proteins such as the thaumatin-like proteins (PR-5) from cherry and apple; proteins homologous to the major birch pollen allergen Bet v 1 (PR-10) from vegetables and fruits; and lipid transfer proteins (PR-14) from fruits and cereals. Allergens other than PR homologs can be allotted to other well-known protein families such as inhibitors of alpha-amylases and trypsin from cereal seeds, profilins from fruits and vegetables, seed storage proteins from nuts and mustard seeds, and proteases from fruits. As more clinical data and structural information on allergenic molecules becomes available, we may finally be able to answer what characteristics of a molecule are responsible for its allergenicity.

  6. Experience in treatment of patients with locally advanced or recurrent breast cancer. Intraarterial infusion chemotherapy combined with radiotherapy

    International Nuclear Information System (INIS)

    For the purpose of local control and breast conservation, intraarterial infusion chemotherapy combined with radiotherapy has been indicated in patients with locally advanced breast cancer both in primary and recurrent cases. The present series, evaluated during the past 4 years, consisted of 15 patients 35-83 years of age, with invasive ductal carcinoma, including 10 with primary breast cancer (stage IIIb: 1, IV: 9) and 5 with postoperative recurrence (stage IIIb: 2, IV: 3). Intraarterial chemotherapy is started, basically infusing ADM 50 mg, MMC 10 mg and CDDP 50 mg into the internal thoracic and/or subclavian artery 1-3 times, followed by reduction surgery (quadrantectomy: 4, wide resection: 2) and radiotherapy to the breast, supraclavicular, parasternal and cervical regions according to tumor extent. Local response after arterial infusion was CR: 2, PR: 10, NC: 3 (response rate: 73% ). The response rate of distant metastases after arterial infusion was 73%. Of 10 patients with primary breast cancer, recurrence was noted in 1. Breast conservation was successful in 8 of 10 patients. One of them, in stage IIIb, has survived for 4.5 years with no evidence of disease and with breast conservation. Five patients with postoperative recurrence showed CR with no recurrence after intraarterial chemotherapy and radiotherapy. Acute skin reaction occurred in 6 patients, and was especially frequent in patients with postoperative recurrence (4 of 5). According to these results, combined therapy affords breast conservation even in patients with locally advanced breast cancer, and improves patient's QOL in stage IV. (author)

  7. Spanking, corporal punishment and negative long-term outcomes: a meta-analytic review of longitudinal studies.

    Science.gov (United States)

    Ferguson, Christopher J

    2013-02-01

    Social scientists continue to debate the impact of spanking and corporal punishment (CP) on negative child outcomes including externalizing and internalizing behavior problems and cognitive performance. Previous meta-analytic reviews have mixed long- and short-term studies and relied on bivariate r, which may inflate effect sizes. The current meta-analysis focused on longitudinal studies, and compared effects using bivariate r and better controlled partial r coefficients controlling for time-1 outcome variables. Consistent with previous findings, results based on bivariate r found small but non-trivial long-term relationships between spanking/CP use and negative outcomes. Spanking and CP correlated .14 and .18 respectively with externalizing problems, .12 and .21 with internalizing problems and -.09 and -.18 with cognitive performance. However, when better controlled partial r coefficients (pr) were examined, results were statistically significant but trivial (at or below pr = .10) for externalizing (.07 for spanking, .08 for CP) and internalizing behaviors (.10 for spanking, insufficient studies for CP) and near the threshold of trivial for cognitive performance (-.11 for CP, insufficient studies for spanking). It is concluded that the impact of spanking and CP on the negative outcomes evaluated here (externalizing, internalizing behaviors and low cognitive performance) are minimal. It is advised that psychologists take a more nuanced approach in discussing the effects of spanking/CP with the general public, consistent with the size as well as the significance of their longitudinal associations with adverse outcomes. PMID:23274727

  8. Changes in leaf proteome profile of Arabidopsis thaliana in response to salicylic acid

    Indian Academy of Sciences (India)

    Riddhi Datta; Ragini Sinha; Sharmila Chattopadhyay

    2013-06-01

    Salicylic acid (SA) has been implicated in determining the outcome of interactions between many plants and their pathogens. Global changes in response to this phytohormone have been observed at the transcript level, but little is known of how it induces changes in protein abundance. To this end we have investigated the effect of 1 mM SA on soluble proteins of Arabidopsis thaliana leaves by proteomic analysis. An initial study at transcript level has been performed on temporal landscape, which revealed that induction of most of the SA-responsive genes occurs within 3 to 6 h post treatment (HPT) and the expression peaked within 24 HPT. Two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF MS/MS analysis has been used to identify differentially expressed proteins and 63 spots have been identified successfully. This comparative proteomic profiling of SA treated leaves versus control leaves demonstrated the changes of many defence related proteins like pathogenesis related protein 10a (PR10a), disease-resistance-like protein, putative late blight-resistance protein, WRKY4, MYB4, etc. along with gross increase in the rate of energy production, while other general metabolism rate is slightly toned down, presumably signifying a transition from ‘normal mode’ to ‘defence mode’.

  9. Personal and behavioral factors associated with bicycling in adults from Curitiba, Parana State, Brazil

    Directory of Open Access Journals (Sweden)

    Marilson Kienteka

    2014-01-01

    Full Text Available Bicycling is an important form of physical activity that can promote health benefits. The objective of this study was to analyze the association between personal and behavioral aspects in transportation bicycling and leisure time bicycling in adults. Data was drawn from a household survey involving 677 adults (53.1% female in Curitiba, Paraná State, Brazil. The prevalence of bicycling was 11.2% for transportation and 16.7% for leisure. The frequency of leisure time bicycling was higher among men (PR = 2.08; p < 0.001, young people < 30 and adults aged between 30 and 39.9, bicycle owners (PR = 8.76; p < 0.001 and among the physically active. Transportation bicycling occurred more frequently among men (PR = 3.63; p < 0.001, individuals aged 30 to 39.9, those with a low socioeconomic status (PR = 5.00; p = 0.006, bicycle owners (PR = 10.2; p < 0.001 and individuals with a negative perception of their quality of life. The prevalence of bicycling is low in Curitiba considering its potential as a means of physical activity. Personal and behavioral factors were associated with each form of bicycling.

  10. Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection

    Directory of Open Access Journals (Sweden)

    Yiming Wang

    2014-12-01

    Full Text Available Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L. in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10 by RT-PCR, and phytoalexins (sakuranetin and momilactone A with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05 in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack.

  11. Induction of Drought Stress Resistance by Multi-Functional PGPR Bacillus licheniformis K11 in Pepper.

    Science.gov (United States)

    Lim, Jong-Hui; Kim, Sang-Dal

    2013-06-01

    Drought stress is one of the major yield affecting factor for pepper plant. The effects of PGPRs were analyzed in relation with drought resistance. The PGPRs inoculated pepper plants tolerate the drought stress and survived as compared to non-inoculated pepper plants that died after 15 days of drought stress. Variations in protein and RNA accumulation patterns of inoculated and non-inoculated pepper plants subjected to drought conditions for 10 days were confirmed by two dimensional polyacrylamide gel electrophoresis (2D-PAGE) and differential display PCR (DD-PCR), respectively. A total of six differentially expressed stress proteins were identified in the treated pepper plants by 2D-PAGE. Among the stress proteins, specific genes of Cadhn, VA, sHSP and CaPR-10 showed more than a 1.5-fold expressed in amount in B. licheniformis K11-treated drought pepper compared to untreated drought pepper. The changes in proteins and gene expression patterns were attributed to the B. licheniformis K11. Accordingly, auxin and ACC deaminase producing PGPR B. licheniformis K11 could reduce drought stress in drought affected regions without the need for overusing agrochemicals and chemical fertilizer. These results will contribute to the development of a microbial agent for organic farming by PGPR. PMID:25288947

  12. Induction of Drought Stress Resistance by Multi-Functional PGPR Bacillus licheniformis K11 in Pepper

    Directory of Open Access Journals (Sweden)

    Jong-Hui Lim

    2013-06-01

    Full Text Available Drought stress is one of the major yield affecting factor for pepper plant. The effects of PGPRs were analyzed in relation with drought resistance. The PGPRs inoculated pepper plants tolerate the drought stress and survived as compared to non-inoculated pepper plants that died after 15 days of drought stress. Variations in protein and RNA accumulation patterns of inoculated and non-inoculated pepper plants subjected to drought conditions for 10 days were confirmed by two dimensional polyacrylamide gel electrophoresis (2D-PAGE and differential display PCR (DD-PCR, respectively. A total of six differentially expressed stress proteins were identified in the treated pepper plants by 2D-PAGE. Among the stress proteins, specific genes of Cadhn, VA, sHSP and CaPR-10 showed more than a 1.5-fold expressed in amount in B. licheniformis K11-treated drought pepper compared to untreated drought pepper. The changes in proteins and gene expression patterns were attributed to the B. licheniformis K11. Accordingly, auxin and ACC deaminase producing PGPR B. licheniformis K11 could reduce drought stress in drought affected regions without the need for overusing agrochemicals and chemical fertilizer. These results will contribute to the development of a microbial agent for organic farming by PGPR.

  13. Effectiveness of boron neutron capture therapy for recurrent head and neck malignancies

    Energy Technology Data Exchange (ETDEWEB)

    Kato, Itsuro [Department of Oral and Maxillofacial Surgery, II Osaka University, Graduate School of Dentistry, Osaka (Japan)], E-mail: katoitsu@dent.osaka-u.ac.jp; Fujita, Yusei [Department of Oral and Maxillofacial Surgery, II Osaka University, Graduate School of Dentistry, Osaka (Japan); Maruhashi, Akira [Radiation Oncology Research Laboratory, Research Reactor Institut, Kyoto University, Osaka (Japan); Kumada, Hiroaki [Japan Atomic Energy Agency, Tokai Research and Development Center, Ibaraki (Japan); Ohmae, Masatoshi [Department of Oral and Maxillofacial Surgery, Izimisano Municipal Hospital, Rinku General Hospital, Izumisano, Osaka (Japan); Kirihata, Mitsunori [Graduate School of Environment and Life Science, Osaka prefectural University, Osaka (Japan); Imahori, Yoshio [Department of Neurosurgery, Kyoto Prefectural University, Kyoto (Japan); CEO of Cancer Intelligence Care Systems, Inc., Tokyo (Japan); Suzuki, Minoru [Radiation Oncology Research Laboratory, Research Reactor Institut, Kyoto University, Osaka (Japan); Sakrai, Yoshinori [Graduate School of Medicine, Sapporo Medical University of Medicine, Hokkaido (Japan); Sumi, Tetsuro; Iwai, Soichi; Nakazawa, Mitsuhiro [Department of Oral and Maxillofacial Surgery, II Osaka University, Graduate School of Dentistry, Osaka (Japan); Murata, Isao; Miyamaru, Hiroyuki [Division of Electrical, Electronic and Information Engineering, Graduate School of Engineering, Osaka University (Japan); Ono, Koji [Radiation Oncology Research Laboratory, Research Reactor Institut, Kyoto University, Osaka (Japan)

    2009-07-15

    It is necessary to explore new treatments for recurrent head and neck malignancies (HNM) to avoid severe impairment of oro-facial structures and functions. Boron neutron capture therapy (BNCT) is tumor-cell targeted radiotherapy that has significant superiority over conventional radiotherapies in principle. We have treated with BNCT 42 times for 26 patients (19 squamous cell carcinomas (SCC), 4 salivary gland carcinomas and 3 sarcomas) with a recurrent and far advanced HNM since 2001. Results of (1) {sup 10}B concentration of tumor/normal tissue ratios (T/N ratio) of FBPA-PET studies were SCC: 1.8-5.7, sarcoma: 2.5-4.0, parotid tumor: 2.5-3.7. (2) Therapeutic effects were CR: 12 cases, PR: 10 cases, PD: 3 cases NE (not evaluated): 1 case. Response rate was 85%. (3) Improvement of QOL such as a relief of severe pain, bleeding, and exudates at the local lesion, improvement of PS, disappearance of ulceration, covered with normal skin and preserved oral and maxillofacial functions and tissues. (4) Survival periods after BNCT were 1-72 months (mean: 13.6 months). Six-year survival rate was 24% by Kaplan-Meier analysis. (5) Adverse-events were transient mucositis and alopecia in most of the cases; three osteomyelitis and one brain necrosis were recognized. These results indicate that BNCT represents a new and promising treatment approach for advanced HNM.

  14. Prevalence, risk factors and social context of active pulmonary tuberculosis among prison inmates in Tajikistan.

    Directory of Open Access Journals (Sweden)

    Daniel E Winetsky

    Full Text Available SETTING: Tuberculosis (TB is highly prevalent in prisons of the former Soviet Union. OBJECTIVE: To understand the behavioral, demographic and biological factors placing inmates in Tajikistan at risk for active TB. DESIGN: We administered a behavioral and demographic survey to 1317 inmates in two prison facilities in Sughd province, Tajikistan along with radiographic screening for pulmonary TB. Suspected cases were confirmed bacteriologically. Inmates undergoing TB treatment were also surveyed. In-depth interviews were conducted with former prisoners to elicit relevant social and behavioral characteristics. RESULTS: We identified 59 cases of active pulmonary TB (prevalence 4.5%. Factors independently associated with increased prevalence of active TB were: HIV-infection by self-report (PR 7.88; 95%CI 3.40-18.28, history of previous TB (PR 10.21; 95%CI 6.27-16.63 and infrequent supplemental nutrition beyond scheduled meals (PR 3.00; 95%CI 1.67-5.62. Access to supplemental nutrition was associated with frequency of visits from friends and family and ability to rely on other inmates for help. CONCLUSION: In prison facilities of Tajikistan, HIV-infection, injection drug use and low access to supplemental nutrition were associated with prevalent cases of active pulmonary TB. Policies that reduce HIV transmission among injection drug users and improve the nutritional status of socially isolated inmates may alleviate the TB burden in Tajikistan's prisons.

  15. Molecular cloning of the apoptosis-related calcium-binding protein AsALG-2 in Avena sativa.

    Science.gov (United States)

    Hoat, Trinh Xuan; Nakayashiki, Hitoshi; Yang, Qian; Tosa, Yukio; Mayama, Shigeyuki

    2013-04-01

    Victorin, the host-selective toxin produced by the fungus Cochliobolus victoriae, induces programmed cell death (PCD) in victorin-sensitive oat lines with characteristic features of animal apoptosis, such as mitochondrial permeability transition, chromatin condensation, nuclear DNA laddering and rRNA/mRNA degradation. In this study, we characterized a calcium-binding protein, namely AsALG-2, which might have a role in the victorin-induced PCD. AsALG-2 is homologous to the Apoptosis-Linked Gene ALG-2 identified in mammalian cells. Northern blot analysis revealed that the accumulation of AsALG-2 transcripts increased during victorin-induced PCD, but not during necrotic cell death. Salicylic acid, chitosan and chitin strongly activated the expression of general defence response genes, such as PR-10; however, neither induced cell death nor the accumulation of AsALG-2 mRNA. Pharmacological studies indicated that victorin-induced DNA laddering and AsALG-2 expression were regulated through similar pathways. The calcium channel blocker, nifedipine, moderately inhibited the accumulation of AsALG-2 mRNA during cell death. Trifluoperazine (calmodulin antagonist) and K252a (serine-threonine kinase inhibitor) reduced the victorin-induced phytoalexin accumulation, but did not prevent the victorin-induced DNA laddering or accumulation of AsALG-2 mRNA. Taken together, our investigations suggest that there is a calcium-mediated signalling pathway in animal and plant PCD in common.

  16. Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera

    Science.gov (United States)

    Singh, Gaurav; Tiwari, Manish; Singh, Surendra Pratap; Singh, Surendra; Trivedi, Prabodh Kumar; Misra, Pratibha

    2016-01-01

    Sterol glycosyltransferases (SGTs) catalyse transfer of glycon moiety to sterols and their related compounds to produce diverse glyco-conjugates or steryl glycosides with different biological and pharmacological activities. Functional studies of SGTs from Withania somnifera indicated their role in abiotic stresses but details about role under biotic stress are still unknown. Here, we have elucidated the function of SGTs by silencing SGTL1, SGTL2 and SGTL4 in Withania somnifera. Down-regulation of SGTs by artificial miRNAs led to the enhanced accumulation of withanolide A, withaferin A, sitosterol, stigmasterol and decreased content of withanoside V in Virus Induced Gene Silencing (VIGS) lines. This was further correlated with increased expression of WsHMGR, WsDXR, WsFPPS, WsCYP710A1, WsSTE1 and WsDWF5 genes, involved in withanolide biosynthesis. These variations of withanolide concentrations in silenced lines resulted in pathogen susceptibility as compared to control plants. The infection of Alternaria alternata causes increased salicylic acid, callose deposition, superoxide dismutase and H2O2 in aMIR-VIGS lines. The expression of biotic stress related genes, namely, WsPR1, WsDFS, WsSPI and WsPR10 were also enhanced in aMIR-VIGS lines in time dependent manner. Taken together, our observations revealed that a positive feedback regulation of withanolide biosynthesis occurred by silencing of SGTLs which resulted in reduced biotic tolerance. PMID:27146059

  17. Connections between the thalamus and the somatosensory areas of the anterior ectosylvian gyrus in the cat.

    Science.gov (United States)

    Burton, H; Kopf, E M

    1984-04-01

    labeled other parts of the posterior complex but failed to label any part of VB except when the injection mass also diffused into SII. Injections into the somatic sensory cortex located lateral to SII, within the lips and depth of the upper bank of the anterior ectosylvian sulcus (AES), heavily labeled the central and posterior portions of Pom. Substantial labeling was noted in the lateral (Pol) and intermediate (Poi) divisions of Po only when the injections involved some part of the auditory areas located immediately posterior to SII. This region included the anterior auditory area that occupies the most posterior part of the AEG and both banks of the immediately adjoining AES. The magnocellular nucleus of the medial geniculate (MGmc) was labeled only when some part of the auditory cortex was injected. The suprageniculate nucleus (SG) was labeled from the insula and lower bank of the AES. These results indicated that the medial (rostral and caudal Pom) and lateral components (Poi, Pol, MGmc) of the posterior complex have separate cortical projection zones to somatic sensory and auditory cortical regions, respectively. SIV and the lateral extent of area 5a located in the medial bank of the anterior suprasylvian sulcus sent projections to the deep layers of the superior colliculus and the ventrolateral periaqueductal gray. No cortico-tectal projections were seen from SII.

  18. Autoportree : [luuletused] / Artur Alliksaar ; [Impressioonid] : Viiu Härm

    Index Scriptorium Estoniae

    Alliksaar, Artur, 1923-1966

    2004-01-01

    õmbeid kottpimedas galeriis ; Ärklituba ; Hommik ei oota õhtut ; Improvisatsioone harfil ; Morbiidseid maastikke ; Sõnad sinule, surm, suurele vaikusele ja iseendale ; Õhtute kollane tolm ; Kõik on kõige peegeldus ; Kas süvenev sügis viib sinugi ?... ; Kolm kosmogoonilist etüüdi ; Interregnum ; Neli etüüdi ; Üks vari laulis apelsinisalus. Ta vaevalt lõpetas, kui unus, kes ja kus ; Ürgmetsas ; Tuleb ingel ja puudutab vett ; Lahkusid, kui loojus kuu ; Rändur on teel... ; Mis saab sellest loomusevalust? ; Läbi selle metsa olen näinud ; Kuskil ajajõe taga ; Aeg

  19. Comparative analysis of cyanobacterial superoxide dismutases to discriminate canonical forms

    Directory of Open Access Journals (Sweden)

    Prabaharan Dharmar

    2007-11-01

    different signatures G-F-H-[ILV]-H-x-[NGT]-[GPDA]-[SQK]-C and G-[GA]-G-G-[AEG]-R-[FIL]-[AG]-C-G, while Ni isoform has an nickel containing SOD domain containing a Ni-hook HCDGPCVYDPA. Conclusion The present analysis unravels the ambiguity among cyanobacterial SOD isoforms. NiSOD is the only SOD found in lower forms; whereas, Fe and Mn occupy the higher orders of cyanobacteria. In conclusion, cyanobacteria harbor either Ni alone or a combination of Fe and Ni or Fe and Mn as their catalytic active metal while Cu/Zn is rare.

  20. 体外循环体腔热灌注顺铂治疗浆膜腔恶性积液的临床观察%Clinical effect of coelom hyperthermia perfusion extracorporeal circulatory system in combination with cisplatin in treatment of malignant effusions

    Institute of Scientific and Technical Information of China (English)

    刘都户; 刘文超; 喻召才; 范黎; 朱延光; 盛蓉; 薛妍; 黄颖

    2009-01-01

    目的:将体外循环体腔热灌注顺铂(DDP)应用于恶性间皮瘤、消化道肿瘤和肺癌所致恶性浆膜腔积液的控制,观察其对恶性积液的治疗效果.方法:选择经病理组织学或细胞学确诊或影像学结合肿瘤标志血清学检查临床诊断的恶性间皮瘤、肝癌、胃癌、胰腺癌、大肠癌和肺癌,同时伴发恶性浆膜腔积液的患者54例,治疗前后客观测量积液量.用TRL型体外循环灌注热化疗治疗系统将DDP应用于浆膜腔恶性积液的控制,治疗间隔48 h,每周期3次,3周为1个周期,治疗1个周期后评价积液控制效果,6周后确认疗效,10周后随访,再次评价疗效.结果:5例恶性间皮瘤1个周期后CR 3例,PR 2例,NE 0例;2例PR患者进行第2个周期治疗后转为CR,6周后确认疗效均为CR;10周后随访有2例复发,积液再次出现.41例消化道肿瘤伴发的恶性胸腔积液和腹水,1个周期后CR 25例,PR 10例,NE 6例,临床有效率为85%;10周后随访确认疗效,41例患者有20例仍为CR,PR 10例,NE 11例,临床获益率为73%.8例肺癌并发的恶性胸腔积液,1个周期后CR 5倒,PR 2例,NE 1例;10周后随访结果为CR 4例,PR 2例,NE 2例,临床有效率为75%.结论:体外循环胸腹腔热灌注DDP区域性热化疗可用于治疗恶性间皮瘤、消化道肿瘤和肺癌伴发的恶性浆膜腔积液,积液控制效果显著.

  1. Preliminary Research of Mechanisms underlying Bacillus cereus AR156-mediated Resistance to Rhizoctonia solani in Rice%蜡质芽孢杆菌AR156防治水稻纹枯病机理初探

    Institute of Scientific and Technical Information of China (English)

    陈刘军; 俞仪阳; 王超; 柯红娇; 刘红霞; 郭坚华

    2014-01-01

    Bacillus cereus AR156, a biocontrol agent against a variety of soil-borne pathogens, could effectively control rice sheath blight in field. Here, the biocontrol efficacy and growth-promoting role of strain AR156 on rice was tested under greenhouse conditions, and the primary mechanism of strain AR156 in controlling rice sheath blight was also studied on both cellular and gene levels. The results revealed that AR156 could control Rhizoctonia solani with an efficiancy of 73.06%, and there was a 14.45% biomass increase in AR156-treated rice plants compared with those non-treated ones. Strain AR156 increased the activities of defensive enzymes SOD, PAL, POD and CAT, and the expression of defense related genes OsPR1b、OsPR10、OsNPR1 and ZB8. In rice plants pretreated with strain AR156 before inoculated with R. solani HNW-21, SOD or PAL activity peak reached in advance by four and two days, respectively, and the accumulation of defense related genes transcripts was rapid and extended. As a result, the resistance of those plants to rice sheath blight was strengthened.%蜡质芽孢杆菌AR156是一种防治多种土传病害的生物制剂,对水稻纹枯病具有较好的防治效果。本研究通过温室试验方面验证菌株 AR156的防病效果和促生作用,从细胞水平和基因水平初步揭示了菌株AR156防治水稻纹枯病的机理。结果表明,菌株AR156对水稻纹枯病的温室防效达73.06%,同时促进水稻生物量增加14.45%。菌株AR156处理提高了水稻植株SOD、PAL、POD和CAT等防御酶活性,增强了OsPR1b、OsPR10、OsNPR1和ZB8等防卫相关基因的表达。接种立枯丝核菌Rhizoctonia solani HNW-21之前用菌株AR156预处理的水稻植株,SOD和PAL分别提前4、2 d出现活性峰;防卫相关基因均提前表达,且表达时间延长,从而提高水稻对纹枯病的抗性。

  2. 转TaPIMP1基因小麦纹枯病抗性的基因表达%Expression profiling of TaPIMP1 gene for resistance to sharp eyespot in transgenic wheat

    Institute of Scientific and Technical Information of China (English)

    周小青; 周淼平; 张增艳; 陈怀谷; 姚金保; 马鸿翔

    2011-01-01

    The TaPIMPl gene had been transferred to wheat cultivar Yangmai 158 in order to increase the resistance to wheat sharp eye spot. To investigate the TaPIMPl gene function and response to wheat sharp eyespot, the expressions in different organs and growth periods of wheat, and the expressions induced by salicylic acid (SA) , methyl jas-roonate (MeJA), ethylene (ET) and inoculation of Rhizoctonia cerealis of TaPIMPl gene in transgenic lines were analyzed by semi-quantitative RT-PCR. The expressions of PR1, PR2, PR5 and PR10 genes were also analyzed. The results showed that the expressions of endogenesis TaPIMPl could be detected in root, leaf, spike and flower in wheat and the expressiondecreased with the plant growth. The expression of TaPIMPl was upregulated by Rhizoctonia cerealis inoculating, and by application of salicylic acid, methyl jasmonate and ethylene. The expressions of PR1 and PR5 in transgenic plants were higher than those in control. It was speculated that salicylic acid cumulated in wheat plant after the infection by Rhizoctonia cerealis, and then the transcript of TaPIMPl was upregulated, which led to the massive expressions of PR1 and PRS in wheat plant andmade the plant against the pathogen. Because of constitutive expression of TaPlMPl, the expressions of PR1 and PR5 in the transgenic plant were stronger and lasted longer than those in the control, and hence the transgenic plant was more resistant to Rhizoctonia cerealis than control.%为了阐明TaPIMP1基因在小麦抗纹枯病反应中所起的作用,以转TaPIMP1基因小麦及其受体亲本为材料,采用半定量RT-PCR方法研究该基因的表达特性及其对水杨酸(SA)、茉莉酸甲酯(MeJA)、乙烯(ET)和纹枯病病菌诱导的应答,分析了受体亲本和转基因植株中PR1、PR2、PR5和PR10的表达异同.结果表明,小麦内源TaPIMP1基因在根、叶、穗、花和籽粒中均能表达,随着植株的生长发育表达量逐渐降低;水杨酸、茉莉酸甲酯

  3. Plant root transcriptome profiling reveals a strain-dependent response during Azospirillum-rice cooperation

    Directory of Open Access Journals (Sweden)

    Benoît eDrogue

    2014-11-01

    Full Text Available Cooperation involving Plant Growth-Promoting Rhizobacteria results in improvements of plant growth and health. While pathogenic and symbiotic interactions are known to induce transcriptional changes for genes related to plant defence and development, little is known about the impact of phytostimulating rhizobacteria on plant gene expression. This study aims at identifying genes significantly regulated in rice roots upon Azospirillum inoculation, considering possible favored interaction between a strain and its original host cultivar. Genome-wide analyses of Oryza sativa japonica cultivars Cigalon and Nipponbare were performed, by using microarrays, seven days post inoculation with A. lipoferum 4B (isolated from Cigalon or Azospirillum sp. B510 (isolated from Nipponbare and compared to the respective non-inoculated condition. A total of 7,384 genes were significantly regulated, which represent about 16 % of total rice genes. A set of 34 genes is regulated by both Azospirillum strains in both cultivars, including a gene orthologous to PR10 of Brachypodium, and these could represent plant markers of Azospirillum-rice interactions. The results highlight a strain-dependent response of rice, with 83 % of the differentially expressed genes being classified as combination-specific. Whatever the combination, most of the differentially expressed genes are involved in primary metabolism, transport, regulation of transcription and protein fate. When considering genes involved in response to stress and plant defence, it appears that strain B510, a strain displaying endophytic properties, leads to the repression of a wider set of genes than strain 4B. Individual genotypic variations could be the most important driving force of rice roots gene expression upon Azospirillum inoculation. Strain-dependent transcriptional changes observed for genes related to auxin and ethylene signalling highlight the complexity of hormone signalling networks in the Azospirillum

  4. Progression-free and overall survival in metastatic castration-resistant prostate cancer treated with abiraterone acetate can be predicted with serial C11-acetate PET/CT.

    Science.gov (United States)

    Farnebo, Jacob; Wadelius, Agnes; Sandström, Per; Nilsson, Sten; Jacobsson, Hans; Blomqvist, Lennart; Ullén, Anders

    2016-08-01

    In this retrospective study, we evaluated the benefit of repeated carbon 11 (C11)-acetate positron emission tomography/computed tomography (PET/CT) to assess response in patients with metastatic castration-resistant prostate cancer (mCRPC) treated with abiraterone acetate (AA).A total of 30 patients with mCRPC were monitored with C11-acetate PET/CT and PSA levels during their treatment with AA. Retrospective evaluation of their response was made after 102 days (median; range 70-155) of treatment. Statistical analyses were employed to detect predictors of progression-free survival (PFS) and overall survival (OS), and potential correlation between serum levels of PSA, standardized uptake values (SUVpeak), and bone lesion index measured from PET were investigated.At follow-up 10 patients exhibited partial response (PR), 10 progressive disease (PD), and 10 stable disease (SD), as assessed by PET/CT. In survival analysis, both PR and PD were significantly associated with PFS and OS. CT response was also associated with OS, but only 19/30 patients demonstrated a lesion meeting target lesion criteria according to RECIST 1.1. No PET/CT baseline characteristic was significantly associated with PFS or OS. A PSA response (reduction in the level by >50%) could also predict PFS and OS. In the subgroup lacking a PSA response, those with PD had significantly shorter OS than those with PR or SD.PFS and OS in patients with mCRPC treated with AA can be predicted from repeated C11-acetate PET/CT. This may be of particular clinical value in patients who do not exhibit a PSA response to treatment. PMID:27495034

  5. Interspecific proteomic comparisons reveal ash phloem genes potentially involved in constitutive resistance to the emerald ash borer.

    Directory of Open Access Journals (Sweden)

    Justin G A Whitehill

    Full Text Available The emerald ash borer (Agrilus planipennis is an invasive wood-boring beetle that has killed millions of ash trees since its accidental introduction to North America. All North American ash species (Fraxinus spp. that emerald ash borer has encountered so far are susceptible, while an Asian species, Manchurian ash (F. mandshurica, which shares an evolutionary history with emerald ash borer, is resistant. Phylogenetic evidence places North American black ash (F. nigra and Manchurian ash in the same clade and section, yet black ash is highly susceptible to the emerald ash borer. This contrast provides an opportunity to compare the genetic traits of the two species and identify those with a potential role in defense/resistance. We used Difference Gel Electrophoresis (DIGE to compare the phloem proteomes of resistant Manchurian to susceptible black, green, and white ash. Differentially expressed proteins associated with the resistant Manchurian ash when compared to the susceptible ash species were identified using nano-LC-MS/MS and putative identities assigned. Proteomic differences were strongly associated with the phylogenetic relationships among the four species. Proteins identified in Manchurian ash potentially associated with its resistance to emerald ash borer include a PR-10 protein, an aspartic protease, a phenylcoumaran benzylic ether reductase (PCBER, and a thylakoid-bound ascorbate peroxidase. Discovery of resistance-related proteins in Asian species will inform approaches in which resistance genes can be introgressed into North American ash species. The generation of resistant North American ash genotypes can be used in forest ecosystem restoration and urban plantings following the wake of the emerald ash borer invasion.

  6. Overexpression of mtDNA-associated AtWhy2 compromises mitochondrial function

    Directory of Open Access Journals (Sweden)

    Abou-Rached Charbel

    2008-04-01

    Full Text Available Abstract Background StWhy1, a member of the plant-specific Whirly single-stranded DNA-binding protein family, was first characterized as a transcription factor involved in the activation of the nuclear PR-10a gene following defense-related stress in potato. In Arabidopsis thaliana, Whirlies have recently been shown to be primarily localized in organelles. Two representatives of the family, AtWhy1 and AtWhy3 are imported into plastids while AtWhy2 localizes to mitochondria. Their function in organelles is currently unknown. Results To understand the role of mitochondrial Whirlies in higher plants, we produced A. thaliana lines with altered expression of the atwhy2 gene. Organellar DNA immunoprecipitation experiments demonstrated that AtWhy2 binds to mitochondrial DNA. Overexpression of atwhy2 in plants perturbs mitochondrial function by causing a diminution in transcript levels and mtDNA content which translates into a low activity level of respiratory chain complexes containing mtDNA-encoded subunits. This lowered activity of mitochondria yielded plants that were reduced in size and had distorted leaves that exhibited accelerated senescence. Overexpression of atwhy2 also led to early accumulation of senescence marker transcripts in mature leaves. Inactivation of the atwhy2 gene did not affect plant development and had no detectable effect on mitochondrial morphology, activity of respiratory chain complexes, transcription or the amount of mtDNA present. This lack of phenotype upon abrogation of atwhy2 expression suggests the presence of functional homologues of the Whirlies or the activation of compensating mechanisms in mitochondria. Conclusion AtWhy2 is associated with mtDNA and its overexpression results in the production of dysfunctional mitochondria. This report constitutes the first evidence of a function for the Whirlies in organelles. We propose that they could play a role in the regulation of the gene expression machinery of organelles.

  7. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction.

    Science.gov (United States)

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de Los Santos, Berta; Arroyo, Francisco T; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen. PMID:27471515

  8. Risk of dengue for tourists and teams during the World Cup 2014 in Brazil.

    Directory of Open Access Journals (Sweden)

    Willem G van Panhuis

    Full Text Available BACKGROUND: This year, Brazil will host about 600,000 foreign visitors during the 2014 FIFA World Cup. The concern of possible dengue transmission during this event has been raised given the high transmission rates reported in the past by this country. METHODOLOGY/PRINCIPAL FINDINGS: We used dengue incidence rates reported by each host city during previous years (2001-2013 to estimate the risk of dengue during the World Cup for tourists and teams. Two statistical models were used: a percentile rank (PR and an Empirical Bayes (EB model. Expected IR's during the games were generally low (<10/100,000 but predictions varied across locations and between models. Based on current ticket allocations, the mean number of expected symptomatic dengue cases ranged from 26 (PR, 10th-100th percentile: 5-334 cases to 59 (EB, 95% credible interval: 30-77 cases among foreign tourists but none are expected among teams. These numbers will highly depend on actual travel schedules and dengue immunity among visitors. Sensitivity analysis for both models indicated that the expected number of cases could be as low as 4 or 5 with 100,000 visitors and as high as 38 or 70 with 800,000 visitors (PR and EB, respectively. CONCLUSION/SIGNIFICANCE: The risk of dengue among tourists during the World Cup is expected to be small due to immunity among the Brazil host population provided by last year's epidemic with the same DENV serotypes. Quantitative risk estimates by different groups and methodologies should be made routinely for mass gathering events.

  9. Expression profiles of hot pepper (Capsicum annuum) genes under cold stress conditions

    Indian Academy of Sciences (India)

    Eul-Won Hwang; Kyung-A Kim; Soo-Chul Park; Mi-Jeong Jeong; Myung-Ok Byun; Hawk-Bin Kwon

    2005-12-01

    In an attempt to determine a cold defense mechanism in plants, we have attempted to characterize changes occurring in the expression of cold-regulated transcript levels in the hot pepper (Capsicum annuum), using cDNA microarray analysis, combined with Northern blot analysis. After analysing a 3.1 K hot pepper cDNA microarray, we isolated a total of 317 cold inducible genes. We selected 42 genes which were up-regulated and three genes which were down-regulated due to cold treatment, for further analysis. Among the 45 genes which appeared to be up-regulated by cold, 19 genes appeared to be simultaneously regulated by salt stress. Among the up-regulated cold-stress genes, we identified a variety of transcription factors, including: a family of 4 ethylene-responsive element binding protein (EREBP, designated CaEREBP-C1 to C4) genes, a bZIP protein (CaBZ1), RVA1, Ring domain protein, HSF1, and the WRKY (CaWRKY1) protein. As mentioned earlier, several genes appeared to be induced not only by cold stress, but also simultaneously by salt stress. These genes included: CaEREBP-C3, CaBZ1, putative trans-activator factor, NtPRp27, malate dehydrogenase, putative auxin-repressed protein, protein phosphatase (CaTPP1), SAR8.2 protein precursor, late-embryogenesis abundant protein 5 (LEA5), DNAJ protein homologue, xyloglucanendo-1,4--D-gucanase precursor, PR10, and the putative non-specific lipid transfer protein StnsLTP.

  10. Co-inoculation with rhizobia and AMF inhibited soybean red crown rot: from field study to plant defense-related gene expression analysis.

    Directory of Open Access Journals (Sweden)

    Xiang Gao

    Full Text Available BACKGROUND: Soybean red crown rot is a major soil-borne disease all over the world, which severely affects soybean production. Efficient and sustainable methods are strongly desired to control the soil-borne diseases. PRINCIPAL FINDINGS: We firstly investigated the disease incidence and index of soybean red crown rot under different phosphorus (P additions in field and found that the natural inoculation of rhizobia and arbuscular mycorrhizal fungi (AMF could affect soybean red crown rot, particularly without P addition. Further studies in sand culture experiments showed that inoculation with rhizobia or AMF significantly decreased severity and incidence of soybean red crown rot, especially for co-inoculation with rhizobia and AMF at low P. The root colony forming unit (CFU decreased over 50% when inoculated by rhizobia and/or AMF at low P. However, P addition only enhanced CFU when inoculated with AMF. Furthermore, root exudates of soybean inoculated with rhizobia and/or AMF significantly inhibited pathogen growth and reproduction. Quantitative RT-PCR results indicated that the transcripts of the most tested pathogen defense-related (PR genes in roots were significantly increased by rhizobium and/or AMF inoculation. Among them, PR2, PR3, PR4 and PR10 reached the highest level with co-inoculation of rhizobium and AMF. CONCLUSIONS: Our results indicated that inoculation with rhizobia and AMF could directly inhibit pathogen growth and reproduction, and activate the plant overall defense system through increasing PR gene expressions. Combined with optimal P fertilization, inoculation with rhizobia and AMF could be considered as an efficient method to control soybean red crown rot in acid soils.

  11. Clinical observation of raltitrexed/bevacizumab combined with irinotecan or oxaliplation for advanced colorectal cancer

    Institute of Scientific and Technical Information of China (English)

    Jianwei Yang; Wei Gao; Jinyuan Lin; Yan Meng; Shuzhen Zhang; Tong Wang

    2014-01-01

    Objective: The aim of the study was to investigate the ef icacy and safety of raltitrexed/bevacizumab in combina-tion with irinotecan or oxaliplation for advanced colorectal cancer as the second-line and second-line above treatments. Meth-ods: Fifteen cases of advanced colorectal cancer were enrol ed to receive regimens including raltitrexed/bevacizumab com-bined with irinotecan or oxaliplation. Two cases were treated with raltitrexed + bavacizumab regimen, 9 cases with raltitrexed+ bavacizumab + irinotecan regimen, and 4 cases with raltitrexed + bevacizumab + oxaliplation regimen. The doses of the drugs were as fol ows: bevacizumab 5 mg/kg ivgtt, d1; raltitrexed 2.0 mg/m2 ivgtt 15 min, d2; irinotecan 180 mg/m2 ivgtt 1 h, d2; and oxaliplatin 85 mg/m2 ivgtt 2 h, d2. Two weeks was a cycle for each regimen. Results: The ef icacy of the 15 patients could be evaluated. Two cases were in PR ,10 cases in SD, 3 cases in PD, the response rate was 13.3%, and the disease control rate was 80.0%. The median progress-free survival was 5.1 months (95% CI: 3.404-6.813 months), and the median overal survival was 11.5 months (95% CI: 8.985-13.930 months). The adverse ef ects included anorexia, nausea/vomit-ing, fatigue, leucopenia, thrombocytopenia, etc, and the main 3-4 grades adverse ef ects were anorexia, nausea/vomiting, fatigue, and thrombocytopenia. Conclusion: Raltitrexed/bevacizumab combined with irinotecan or oxaliplatin as the second-line and second-line above treatments for advanced colorectal cancer has high disease control rates, and the adverse ef ect is wel tolerated. The combined regimen can be recommended as a phase III clinical research and second-line and second-lines above treatments for advanced colorectal cancer.

  12. Treatment Outcome of Mucosa Associated Lymphoid Tissue (MALT) Marginal Zone Non-Hodgkin's Lymphoma. Single Institutional Experience

    International Nuclear Information System (INIS)

    To evaluate the treatment outcome in patients with mucosa associated lymphoid tissue (MALT) lymphoma in terms of response to treatment, progression-free and overall survivals as well as prognostic factors. Patients and Methods: Between 1995 and 2002,40 patients with clinical stages (CS) I-IV MALT lymphoma were treated at NEMROCK. The progression free survival (PFS) and overall survival (OAS) were calculated using the Kaplan Meier technique. Thirty-one patients (77.5%) had CS I-II and 9 (22.5%) had CS III-IV disease. Twenty of the 31 CS I-II patients received radiation therapy alone, five patients received chemotherapy, while three patients were treated by triple therapy (Amoxicillin, Omeperazole, Clarithromycin). Among the 9 CS III-IV patients, treatment included chemotherapy alone (6 patients), chemo radiation (2 patients) and surgery (one patient). The median followup period was 40 months. 19 out of twenty patients with CS I-II treated by radiation therapy alone had a 95% response rate (CR 85% - PR 10%). Among the study population (40 patients), the 5 year OAS and PFS were 86% and 66%, respectively. The 5 year OAS was 86% and PFS was 72% among CS III patients; the corresponding estimates in CS III-IV patients were 70% and 28%, respectively. Using multivariate analysis, there was a significant correlation between the stage of the disease, site of presentation (non GIT) and the overall survival. Modest doses of radiation therapy provide better local control in patients with early stage MALT lymphoma. The poor PFS in advanced staged disease suggests the need for further clinical trials evaluating novel drug approaches taking into consideration the biological behavior and the indolent nature of such disease entity

  13. 妊娠晚期心电图的异常分析%Analysis of ECG Abnormalities in Late Pregnancy

    Institute of Scientific and Technical Information of China (English)

    吐逊古•阿西木; 迪丽拜尔•克尤木

    2014-01-01

    Objective The ecg changes in late pregnancy women and the relationship between the clinical and treatment method.Methods The guangdong state built ECG- 9803 type routine eeg, ECG machine, analysis the change of electrocardiogram (ECG), 148 cases of late pregnancy women.Results The types of abnormal ecg in 148 cases of late pregnancy women were,electrical axis left 75 cases,40 cases of sinus tachycardia,sinus arrhythmia,12 cases were shortened period between P-R 10 cases,5 cases of premature beat,QRS low voltage 4 cases,S T-T change in 2 cases.Conclusion See more quite late pregnancy women abnormal ecg, should be focus on change, timely intervention treatment when necessary, to ensure life safety of pregnant women and fetus.%目的探讨妊娠晚期妇女心电图改变与临床的关系及处理方法。方法采用美国M 1772A型心电图机进行常规描记,分析148例妊娠晚期女性的心电图的改变。结果148例孕晚期妇女中心电图异常的种类分别是,电轴左偏75例,窦性心动过速40例、窦性心律不齐12例,P-R间期缩短10例、早搏5例、QRS低电压4例,ST-T改变2例。结论妊娠晚期妇女心电图异常比较多见,应予关注改变,必要时及时进行干预治疗,以保障孕妇和胎儿的生命安全。

  14. Studies on the mechanism of resistance to Bipolaris sorokiniana in the barley lesion mimic mutant bst1.

    Science.gov (United States)

    Persson, Mattias; Falk, Anders; Dixelius, Christina

    2009-09-01

    SUMMARY The Bipolaris sorokiniana tolerant 1 (bst1) barley mutant is derived from fast neutron-irradiated seeds of wild-type Bowman(Rph3). The induced mutation was genetically localized to a position on chromosome 5HL distal to the centromere using amplified fragment length polymorphism markers. In addition, the defence responses and related gene expression in the bst1 mutant after fungal challenge were compared with those occurring in wild-type plants. Hydrogen peroxide generation, determined by 3,3-diaminobenzidine staining, revealed a clearly reduced level of bst1, compared with the wild-type, during the entire experimental time: 8-120 h post-inoculation (hpi). At 48 hpi, the wild-type samples displayed twice as much fungal mass and three times greater H(2)O(2) production than bst1. At the same time, staining of B. sorokiniana showed less fungal growth in the spontaneous lesions of bst1 compared with the wild-type. Monitoring of defence-related genes at 48 hpi demonstrated strong expression of PR-1a, PR-2, PR-5 and PR-10 in bst1. A gene coding for a unique oxidoreductase enzyme, designated as HCP1, was expressed at much higher levels in inoculated leaves of the bst1 mutant than in those of the wild-type plant. Taken together, the results suggest that the defence to B. sorokiniana largely relies on salicylic acid-responsive pathogenesis-related (PR) genes, as well as selected reactive oxygen species and unknown HCP1-associated factors.

  15. Induction of host defences by Rhizobium during ineffective nodulation of pea (Pisum sativum L.) carrying symbiotically defective mutations sym40 (PsEFD), sym33 (PsIPD3/PsCYCLOPS) and sym42.

    Science.gov (United States)

    Ivanova, Kira A; Tsyganova, Anna V; Brewin, Nicholas J; Tikhonovich, Igor A; Tsyganov, Viktor E

    2015-11-01

    Rhizobia are able to establish a beneficial interaction with legumes by forming a new organ, called the symbiotic root nodule, which is a unique ecological niche for rhizobial nitrogen fixation. Rhizobial infection has many similarities with pathogenic infection and induction of defence responses accompanies both interactions, but defence responses are induced to a lesser extent during rhizobial infection. However, strong defence responses may result from incompatible interactions between legumes and rhizobia due to a mutation in either macro- or microsymbiont. The aim of this research was to analyse different plant defence reactions in response to Rhizobium infection for several pea (Pisum sativum) mutants that result in ineffective symbiosis. Pea mutants were examined by histochemical and immunocytochemical analyses, light, fluorescence and transmission electron microscopy and quantitative real-time PCR gene expression analysis. It was observed that mutations in pea symbiotic genes sym33 (PsIPD3/PsCYCLOPS encoding a transcriptional factor) and sym40 (PsEFD encoding a putative negative regulator of the cytokinin response) led to suberin depositions in ineffective nodules, and in the sym42 there were callose depositions in infection thread (IT) and host cell walls. The increase in deposition of unesterified pectin in IT walls was observed for mutants in the sym33 and sym42; for mutant in the sym42, unesterified pectin was also found around degrading bacteroids. In mutants in the genes sym33 and sym40, an increase in the expression level of a gene encoding peroxidase was observed. In the genes sym40 and sym42, an increase in the expression levels of genes encoding a marker of hypersensitive reaction and PR10 protein was demonstrated. Thus, a range of plant defence responses like suberisation, callose and unesterified pectin deposition as well as activation of defence genes can be triggered by different pea single mutations that cause perception of an otherwise

  16. EAACI Molecular Allergology User's Guide.

    Science.gov (United States)

    Matricardi, P M; Kleine-Tebbe, J; Hoffmann, H J; Valenta, R; Hilger, C; Hofmaier, S; Aalberse, R C; Agache, I; Asero, R; Ballmer-Weber, B; Barber, D; Beyer, K; Biedermann, T; Bilò, M B; Blank, S; Bohle, B; Bosshard, P P; Breiteneder, H; Brough, H A; Caraballo, L; Caubet, J C; Crameri, R; Davies, J M; Douladiris, N; Ebisawa, M; EIgenmann, P A; Fernandez-Rivas, M; Ferreira, F; Gadermaier, G; Glatz, M; Hamilton, R G; Hawranek, T; Hellings, P; Hoffmann-Sommergruber, K; Jakob, T; Jappe, U; Jutel, M; Kamath, S D; Knol, E F; Korosec, P; Kuehn, A; Lack, G; Lopata, A L; Mäkelä, M; Morisset, M; Niederberger, V; Nowak-Węgrzyn, A H; Papadopoulos, N G; Pastorello, E A; Pauli, G; Platts-Mills, T; Posa, D; Poulsen, L K; Raulf, M; Sastre, J; Scala, E; Schmid, J M; Schmid-Grendelmeier, P; van Hage, M; van Ree, R; Vieths, S; Weber, R; Wickman, M; Muraro, A; Ollert, M

    2016-05-01

    The availability of allergen molecules ('components') from several protein families has advanced our understanding of immunoglobulin E (IgE)-mediated responses and enabled 'component-resolved diagnosis' (CRD). The European Academy of Allergy and Clinical Immunology (EAACI) Molecular Allergology User's Guide (MAUG) provides comprehensive information on important allergens and describes the diagnostic options using CRD. Part A of the EAACI MAUG introduces allergen molecules, families, composition of extracts, databases, and diagnostic IgE, skin, and basophil tests. Singleplex and multiplex IgE assays with components improve both sensitivity for low-abundance allergens and analytical specificity; IgE to individual allergens can yield information on clinical risks and distinguish cross-reactivity from true primary sensitization. Part B discusses the clinical and molecular aspects of IgE-mediated allergies to foods (including nuts, seeds, legumes, fruits, vegetables, cereal grains, milk, egg, meat, fish, and shellfish), inhalants (pollen, mold spores, mites, and animal dander), and Hymenoptera venom. Diagnostic algorithms and short case histories provide useful information for the clinical workup of allergic individuals targeted for CRD. Part C covers protein families containing ubiquitous, highly cross-reactive panallergens from plant (lipid transfer proteins, polcalcins, PR-10, profilins) and animal sources (lipocalins, parvalbumins, serum albumins, tropomyosins) and explains their diagnostic and clinical utility. Part D lists 100 important allergen molecules. In conclusion, IgE-mediated reactions and allergic diseases, including allergic rhinoconjunctivitis, asthma, food reactions, and insect sting reactions, are discussed from a novel molecular perspective. The EAACI MAUG documents the rapid progression of molecular allergology from basic research to its integration into clinical practice, a quantum leap in the management of allergic patients. PMID:27288833

  17. Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction

    Science.gov (United States)

    Amil-Ruiz, Francisco; Garrido-Gala, José; Gadea, José; Blanco-Portales, Rosario; Muñoz-Mérida, Antonio; Trelles, Oswaldo; de los Santos, Berta; Arroyo, Francisco T.; Aguado-Puig, Ana; Romero, Fernando; Mercado, José-Ángel; Pliego-Alfaro, Fernando; Muñoz-Blanco, Juan; Caballero, José L.

    2016-01-01

    Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria × ananassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutatum spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen. PMID:27471515

  18. Benzylisoquinoline alkaloid metabolism: a century of discovery and a brave new world.

    Science.gov (United States)

    Hagel, Jillian M; Facchini, Peter J

    2013-05-01

    Benzylisoquinoline alkaloids (BIAs) are a structurally diverse group of plant specialized metabolites with a long history of investigation. Although the ecophysiological functions of most BIAs are unknown, the medicinal properties of many compounds have been exploited for centuries. These include the narcotic analgesics codeine and morphine, the antimicrobial agents sanguinarine and berberine, and the antitussive and anticancer drug noscapine. BIA biosynthesis involves a restricted number of enzyme types that catalyze landmark coupling reactions and subsequent functional group modifications. A pathogenesis-related (PR)10/Bet v1 'Pictet-Spenglerase', several O-methyl-, N-methyl- and O-acetyltransferases, cytochromes P450, FAD-dependent oxidases, non-heme dioxygenases and NADPH-dependent reductases have been implicated in the multistep pathways leading to structurally diverse alkaloids. A small number of plant species, including opium poppy (Papaver somniferum) and other members of the Ranunculales, have emerged as model systems to study BIA metabolism. The expansion of resources to include a wider range of plant species is creating an opportunity to investigate previously uncharacterized BIA pathways. Contemporary knowledge of BIA metabolism reflects over a century of research coupled with the development of key innovations such as radioactive tracing, enzyme isolation and molecular cloning, and functional genomics approaches such as virus-induced gene silencing. Recently, the emergence of transcriptomics, proteomics and metabolomics has expedited the discovery of new BIA biosynthetic genes. The growing repository of BIA biosynthetic genes is providing the parts required to apply emerging synthetic biology platforms to the development of production systems in microbes as an alternative to plants as a commecial source of valuable BIAs.

  19. Prospecting For Magnetite Ore Deposits With A Innovative Sensor's of Unique Fundamentally New Magnetometer.

    Science.gov (United States)

    Emelianenko, T. I.; Tachaytdinov, R. S.; Sarichev, V. F.; Kotov, B. V.; Susoeva, G. N.

    erosional truncation level determination(Patent for invention N 2148844 dated 10.05.2000,M.,2000). Emelianenko T.I.,Tachaytdinov R.S.,Sarichev V.F.,Kotov B.V.,Susoeva G.N.2000.Fundamentally new magnetometer -locator for search magnetite ores (Abstracts of 31 International Geological Congress -Rio-de-Janeiro Brasil)at http www:// 31.igc.org. Emel ianenko T.I.,Matveev A.A. 2000.New geochemical method of quantity estimation of magnetite deposits(Abstracts of 31 International Geological Congress in Rio de Janeiro Brasil)at htt :www.31.igc.org. Emelianenko T.I.,Matveev A.A.2001.Fundamentally new geochemical method of quantitative of magnetite ore deposits(Abstracts of 20 International Geochemical Exploration Simposium,Santiago de Chile)at http ://aeg.org. Emelianenko T.I.,Matveev A.A.2001.Fundamentally new geochemical method of hypsometrical level of magnetite deposits ore zone.(Abstracts of 20 International Geochemical Exploration Symposium Santiago de Chile).at http:www.aeg.org. Nikitskiye V.E.,Glebovskiye J.S..Magnitoprospecting.M.N.1980. Nikitskiye V.E.,Glebovskiye J.S. Magnitoprospecting.M.,N.,1990. .Grinevitch G.I.Magnitoprospecting.M.,N.,1974.

  20. Descobrindo a Doença de Parkinson: impacto para o parkinsoniano e seu familiar Descubriendo la Enfermedad de Parkinson: impacto para el parkinsoniano y su familiar Diagnosed with Parkinson's Diesease: impact on patients and family members

    Directory of Open Access Journals (Sweden)

    Fabiana Magalhães Navarro Peternella

    2009-02-01

    Full Text Available Estudo de natureza qualitativa, com o objetivo de compreender o impacto do diagnóstico de Parkinson para o individuo e família. Os dados foram coletados no período de Outubro a Dezembro de 2007, junto a 20 indivíduos residentes em Maringá - PR, sendo dez parkinsonianos e seu familiar mais próximo, por meio de entrevista semi-estruturada. Na análise dos dados emergiram dois temas: "Descobrindo-se portador de doença de Parkinson" e "Percalços e sentimentos após o diagnostico", os quais revelam que a falta de conhecimento sobre a doença ainda é muito grande e que a qualidade do primeiro contato com o diagnóstico é crucial na determinação e comportamento dos indivíduos frente à doença.Estudio de naturaleza cualitativa, desarrollado con el objetivo de comprender el impacto del diagnóstico de Parkinson para el individuo y familia. Los datos fueron recogidos en el período de Octubre a Diciembre de 2007, junto a 20 individuos residentes en Maringá - PR, siendo diez parkinsonianos y su familiar más próximo, por medio de entrevista semiestructurada. En el análisis de los datos emergieron dos temas: "Descubriéndose portador de enfermedad de Parkinson: una larga trayectoria" y "Percances y sentimientos después del diagnóstico", los cuales revelan que la falta de conocimiento sobre la enfermedad aún es muy grande en la población en general y que la calidad del primer contacto con el diagnóstico es crucial en la determinación y comportamiento de los individuos frente la enfermedad.This was a qualitative nature, developed with the objective of understanding the impact of a Parkinson's diagnosis on the individual and the family. Data were collected between October and December 2007, from 20 individuals residing in Maringá, PR - 10 Parkinson's patients and their closest family member - using semi-structured interviews. From the analysis of the data, two themes emerged: "Coming to terms as a Parkinson's patient: a long path" and

  1. The Bet v 1 fold: an ancient, versatile scaffold for binding of large, hydrophobic ligands

    Directory of Open Access Journals (Sweden)

    Breiteneder Heimo

    2008-10-01

    Full Text Available Abstract Background The major birch pollen allergen, Bet v 1, is a member of the ubiquitous PR-10 family of plant pathogenesis-related proteins. In recent years, a number of diverse plant proteins with low sequence similarity to Bet v 1 was identified. In addition, determination of the Bet v 1 structure revealed the existence of a large superfamily of structurally related proteins. In this study, we aimed to identify and classify all Bet v 1-related structures from the Protein Data Bank and all Bet v 1-related sequences from the Uniprot database. Results Structural comparisons of representative members of already known protein families structurally related to Bet v 1 with all entries of the Protein Data Bank yielded 47 structures with non-identical sequences. They were classified into eleven families, five of which were newly identified and not included in the Structural Classification of Proteins database release 1.71. The taxonomic distribution of these families extracted from the Pfam protein family database showed that members of the polyketide cyclase family and the activator of Hsp90 ATPase homologue 1 family were distributed among all three superkingdoms, while members of some bacterial families were confined to a small number of species. Comparison of ligand binding activities of Bet v 1-like superfamily members revealed that their functions were related to binding and metabolism of large, hydrophobic compounds such as lipids, hormones, and antibiotics. Phylogenetic relationships within the Bet v 1 family, defined as the group of proteins with significant sequence similarity to Bet v 1, were determined by aligning 264 Bet v 1-related sequences. A distance-based phylogenetic tree yielded a classification into 11 subfamilies, nine exclusively containing plant sequences and two subfamilies of bacterial proteins. Plant sequences included the pathogenesis-related proteins 10, the major latex proteins/ripening-related proteins subfamily, and

  2. 吉西他滨联合卡培他滨治疗转移性三阴性乳腺癌的临床观察%Clinical Outcomes of Gemcitabine Combined Capecitabine with Metastatic Triple-negative Breast Cancer

    Institute of Scientific and Technical Information of China (English)

    李景; 高俊峰

    2013-01-01

    Objective:To observe the ef ect and side reaction of Gemcitabine combined capecitabine inmetastatic triple-negative breast cancer. Method:28 patients with metastatic triple-negative breast cancer were enrol ed. Capecitabine was oral dose with 2000mg/m2 per day though day 1 to day 14 and Gemcitabine injected with 1000mg/m2 at day 1 and day 8. Every 2 weeks repeated. Results:The outcomes in 28 patients included completed response (n=2,7.14%),partial response (n=10,35.7%) , stable disease (n=10,35.7%),progressive disease (n=6,21.4%) Total ef ective rate was 42.9%. Conclusion: Gemcitabine combined capecitabine was ef ective plan with with metastatic triple-negative breast cancer after the patients were failed with anthracycline and/or taxanes.%目的观察吉西他滨联合卡培他滨治疗蒽环及紫杉类药物耐药的转移性三阴性乳腺癌的疗效及不良反应。方法28例转移性三阴性乳腺癌患者,卡培他滨2000mg/m2·d,第1~14d,早晚饭后30min,2次/d口服;吉西他滨1000mg/m2,第1、8d,3周天为1周期。结果28例患者中,CR2例(7.14%),PR 10例(35.7%),SD 10例(35.7%),PD6例(21.4%),总有效率(CR+PR)为42.9%。结论卡培他滨联合吉西他滨治疗蒽环类和(或)紫杉类耐药转移性三阴性乳腺癌患者疗效较好,不良反应可以耐受.可以作为蒽环类和(或)紫杉类耐药的晚期三阴性乳腺癌患者的一个有效解救方案。

  3. The effect of high polyphenol oxidase grass silage on metabolism of polyunsaturated fatty acids and nitrogen across the rumen of beef steers.

    Science.gov (United States)

    Lee, M R F; Theobald, V J; Gordon, N; Leyland, M; Tweed, J K S; Fychan, R; Scollan, N D

    2014-11-01

    Polyphenol oxidase (PPO) activity in red clover (Trifolium pratense) has been reported to reduce both proteolysis and lipolysis, resulting in greater N use efficiency and protection of PUFA across the rumen. Although high levels of PPO have been reported in grasses such as cocksfoot (orchard grass; Dactylis glomerata), no in vivo research has determined whether grass PPO elicits the same response as red clover PPO. To test the hypothesis that silage ensiled from grass with high levels of PPO protects N and PUFA across the rumen, 6 steers with ruminal and duodenal cannulas were offered cocksfoot silage (CO; high-PPO grass), perennial ryegrass silage (PR; Lolium perenne; low-PPO grass), or red clover silage (RC; high-PPO control) at 16 g DM/kg BW daily with the experiment consisting of two 3 × 3 Latin squares with 21-d periods, consisting of 12 d of diet adaptation, 6 d of duodenal marker infusion, 2 d of duodenal sampling, and 1 d of ruminal sampling. All silages were well preserved, with DM of 34.4, 55.3, and 45.4% for CO, PR, and RC. Activity of PPO in silages was low due to deactivation but was greater in CO than either PR or RC (0.15 vs. 0.05 and 0.08 μkatal/g DM). Protein-bound phenol (mg/g DM) as a measure of the degree of oxidation and an indication of PPO protection was greatest for RC (15.9) but comparable for PR (10.1) and CO (12.2). Biohydrogenation of C18 PUFA was significantly lower on RC compared to the 2 grass silages with CO greater than PR. Despite lower levels of total fatty acid intake and subsequent duodenal flow, CO resulted in greater levels of phytanic acid and total branched and odd chain fatty acids in duodenal digesta than RC or PR. Ruminal ammonia concentration was greatest for RC, with no difference between the grasses. Duodenal flow of microbial N and efficiency of microbial protein synthesis were lowest for CO and comparable for RC and PR. The CO (high-grass PPO) did not result in elevated levels of C18 PUFA escaping the rumen or

  4. Prevalence and Correlates of HIV Infection among Street Boys in Kisumu, Kenya.

    Directory of Open Access Journals (Sweden)

    Ariella Goldblatt

    Full Text Available Despite their perceived vulnerability to HIV, East African street youth have been neglected in HIV prevention research. We examined HIV seroprevalence and correlates of HIV infection in a sample of male street youth in Kisumu, Kenya.We enrolled a street-recruited sample of 13-21 year old street youth. Participants completed a survey followed by voluntary HIV counseling and testing. Survey items included demographics, homelessness history, survival activities, sexual behavior and substance use. We examined the relationship between predictor variables, markers of coercion and marginalization and HIV.The sample included 296 males. Survival activities included garbage picking (55%, helping market vendors (55%, begging (17%, and working as porters (46% or domestic workers (4%. Forty-nine percent of participants reported at least weekly use of alcohol and 32% marijuana. Forty-six percent of participants reported lifetime inhalation of glue and 8% fuel. Seventy-nine percent of participants reported lifetime vaginal sex, 6% reported lifetime insertive anal sex and 8% reported lifetime receptive anal sex. Twelve (4.1%; 95% CI: 2.3-7.0 participants tested positive for HIV. Of those, all had been on the street for at least one year and all had engaged in vaginal sex. Occupations placing youth at particular risk of coercion by adults, including helping market vendors (prevalence ratio (PR = 8.8; 95% CI: 1.2-67.5 and working as domestic workers (PR = 4.6; 95% CI: 1.1-19.0, were associated with HIV infection. Both insertive anal sex (PR = 10.2; 95% CI: 3.6-29.4 and receptive anal sex (PR = 3.9; 95% CI: 1.1-13.4 were associated with HIV infection. Drug use, begging, and garbage picking were not associated with HIV infection.Although HIV prevalence in our sample of street youth is comparable to that of similarly-aged male youth in Nyanza Province, our findings highlight behavioral factors associated with HIV infection that offer opportunities for targeted

  5. Clinical Observation of HIFU in Combination with Chemotherapy on Recurrent or Metastatic Peritoneo-Pelvic Malignant Tumors%HIFU合并化疗治疗腹盆腔复发或转移癌的临床观察

    Institute of Scientific and Technical Information of China (English)

    许新华; 付向阳; 李松; 陈燕; 张小红; 薛峰; 李道俊; 王翠华

    2006-01-01

    目的:探讨高能聚焦超声刀(High intensity focused ultrasound,HIFU)合并化疗治疗腹盆腔复发或转移癌的疗效及不良反应.方法:选取既往病史有过手术和/或放、化疗史,病理诊断明确的腹盆腔复发或转移癌68例,随机分为治疗组(36例)和对照组(32例),前者采用HIFU+化疗,后者单纯用化疗,均在完成2周期化疗后评价其有效率、临床受益反应,并至少随访1年.结果:治疗组完全缓解(CR)1例,部分缓解(PR)24例,总有效率69.4%(25/36),临床受益率(CBR)为83.3%(30/36),平均生存期为9.1±3.1个月;对照组CR 0例,PR 10例,总有效率31.3%(10/32),CBR为56.3%(18/32),平均生存期为6.8±2.4个月;两组比较有显著性意义(P<0.01、P<0.05、P<0.01),主要不良反应为骨髓抑制和消化道反应,两组间比较无统计学差异,治疗组无脏器穿孔、大出血、胰漏、腹膜炎等并发症发生.结论:HIFU合并化疗治疗腹盆腔复发或转移癌可取得较高的局控率和临床受益率,并能延长其生存期,对于无手术和(或)放疗适应症的晚期腹盆腔恶性肿瘤不失为一种安全、有效的高姑息治疗手段.

  6. Clinical study of CAG regimen combined Decitabine in treatment of elderly patients with moderate and high risk myelodysplastic syndrome and acute myeloid leukemia%地西他滨联合小剂量化疗治疗老年中高危骨髓增生异常综合征及急性髓系白血病的临床观察

    Institute of Scientific and Technical Information of China (English)

    徐瑜; 沙颖豪; 谢彦晖

    2013-01-01

    Objective To observe the clinical efficacy and adverse events of treatment for elderly patients with moderate and high-risk myelodysplastic syndrome (MDS) and acute myelocytic leukemia (AMD with Decitabine (25mg/d, d1-3) combined with CAG (G-CSF300ug/d, d4-13; aclarubicin10mg/d, d4,6,8,10,12; Ara-C25mg/d, d4-13) regimen. Methods Decitabine combined with CAG regimen were used to treat 10 elderly patients with MDS and AML. The outcome was evaluated after 1 course of treatment. Results For ten patients, 3/10(30%) achieved completely remission (CR), 4/10 (40%) marrow completely remission (mCR), 1/10 (10%) partial remission (PR). The overall response rate was 80%. Most patients can withstand adverse reactions occurred mainly for the arrest of bone marrow. Conclusion In older patients, medium and high-risk MDS and AML treated with Decitabine combined with CAG regimen is safe and effective.%目的 观察地西他滨联合CAG方案治疗老年中高危MDS及AML的临床疗效及不良反应.方法 应用地西他滨联合CAG方案治疗MDS及AML患者10例.1个疗程后评估疗效.结果 10例患者,其中3例获得完全缓解(CR) 30%,4例为部分缓解(PR) 10%,1例获得骨髓缓解(mCR)40%.总有效率为80%.大多数患者出现了可以耐受的不良反应,主要表现为骨髓抑制.结论 地西他滨联合CAG方案治疗中高危MDS和老年AML有较好的疗效和安全性.

  7. Effect of estradiol combined with different doses of puerarin on osteoporosis of ovariectomized rats%雌激素与不同剂量的葛根素联合对去卵巢大鼠骨质疏松的影响

    Institute of Scientific and Technical Information of China (English)

    黄海玲; 李海; 王金花; 黎飚; 解继胜; 刘洁

    2011-01-01

    目的:观察雌二醇与不同剂量的葛根素组合对去卵巢大鼠骨组织的影响,为中西医结合的雌激素与葛根素最佳组合剂量治疗Ⅰ型骨质疏松症提供实验依据.方法:5月龄健康雌性大白鼠70只,分成7个实验组(每组10只),除假手术组外均建立去卵巢动物模型.葛根素-50组(Pr-50),皮下注射葛根素50 mg/kg,1次/天;雌二醇-200组(E2-200),皮下注射雌二醇200 μg/kg,2次/周;雌二醇+葛根素- 10组(E2+ Pr-10),皮下注射雌二醇100 μg/kg,2次/周,葛根素10 mg/kg,1次/天;雌二醇+葛根素-25组(E2+Pr-25),皮下注射雌二醇100μg/kg,2次/周和葛根素25mg/kg,1次/天;雌二醇+葛根素-40组(E2+ Pr -40),皮下注射雌二醇100 μg/kg,2次/周和葛根素40 mg/kg,1次/天.各实验组在第10周和第20周,随机取5只大鼠取左股骨切片观察骨组织,取右股骨测量骨密度、骨钙、骨磷含量.结果:OVX组在第10周和第20周的骨密度和骨钙、骨磷明显低于假手术组和5个用药组(P<0.05),骨组织呈骨质疏松的病理改变;E2+ Pr-25组在第10周和第20周的骨密度和骨钙、骨磷明显高于E2 +P r- 10组(P<0.05);而与假手术组、Pr-50、E2- 200和E2+Pr-40相比无统计学差异(P>0.05).结论:E2+Pr-25组葛根素与雌二醇的组合剂量治疗去卵巢大鼠骨质疏松效果明显优于E2+Pr-10组的小剂量,与假手术组、相对较大剂量的E2+ Pr-40组和单一较大剂量Pr -50、E2-200组相比治疗效果无明显差异.%Objective: To observe the effect of estradiol combined with different doses of puerarin on osteoporosis of ovariectomized rats, provide a experimental basis for finding out the optimal dose of puerarin combined with estradiol to treat type I osteoporosis. Methods: 70 5 - month - old healthy female rats were divided into 7 experimental groups, 10 rats in each group, the models of ovariectomized rats were established in different groups except sham - operated group. The rats in

  8. Effects of different nerve autografts on greater auricular nerve deficit in rabbits

    Institute of Scientific and Technical Information of China (English)

    Shaozong Chen

    2007-01-01

    BACKGROUND: Autograft is commonly used to repair nerve deficit. Usually, the choice of donor nerves is based on their similarities in form and structures to the injured nerves. For the reason, the cutaneous antebrachii lateralis nerve is currently considered the most suited for digital nerve repair. OBJECTIVE: To compare early nerve regeneration after transplantation of three different autografts: the greater auricular nerve (CAN), the saphenous nerve (SN) and the lateral femoral cutaneous nerve (LFCN). DESIGN: Observational contrast study. SETTING: Department of Plastic Surgery and Burns, Tangdu Hospital, Fourth Military Medical University of Chinese PLA.MATERIALS: A total of 42 New Zealand rabbits, of both genders, 12 - 14 months old and weighing 2.0 -2.5 kg, were used in this study. In addition, Moller-spetra 900 operating microscope (Germany), Olympus BX 51 microscope, DP 70 image collecting System (Japan), BL-420E+ Biologic function testing System (China), JEM-100 electron microscope (Japan), Reichet-JunG820 Cryostat (Swiss), and Libror-AEG-120 precision analytical Balance (Japan) were also used in this study.METHODS: The experiment was carried out in the Department of Plastic Surgery and Burns, Tangdu Hospital, Fourth Military Medical University of Chinese PLA from April to November 2005. After anaesthesia, the GAN were dissected bilaterally and a 1.2 cm deficit was made in each nerve. The animals were randomly divided into three groups, including GAN group, SN group and LFCN group with 14 in each group. ①Nerve pinch test: At 1,2, and 4 weeks after operation, three animals in each group were tested. The nerve grafts, along with the proximal and distal GAN segments were exposed and pinched with microsurgical forceps in distal-proximal orientations. The distance between the proximal anastomosis site and the most distal point, where the pinch evoked an ear contraction response, was measured as distance of nerve regeneration. ②Computer image analysis: At 4

  9. 多层螺旋CT灌注成像对肺癌化疗效果的临床评价研究%Study on the Clinical Curative Effect Evaluation of Multi-slice Spiral CT Perfusion Imaging in Lung Cancer Chemo-therapy

    Institute of Scientific and Technical Information of China (English)

    白萍

    2014-01-01

    Objective To discuss the change of perfusion parameters before and after lung cancer chemotherapy, evaluate the value of CT perfusion imaging in lung cancer chemotherapy curative effect evaluation. Methods 30 cases meeted a criterion from January to February 2013, which of chemotherapy effect and CT perfusion parameters were analysed before and after chemo-therapy. Results After two cycles of chemotherapy, there were 2 cases with PR, 10 cases with CR, 13 cases with SD, 5 cases with PD in 30 patients. Before chemotherapy BF value in effective group was higher than the invalid group, and the difference was statistically significant (P0. 05). Compared with before treatment, BF value descended and MTT value elevated in effective group (P0. 05). BF, BV, MTT and PS value had no significant difference in valid group before and after treat-ment(P>0. 05). Conclusion CT perfusion imaging can reflect blood supply of non-small cell lung cancer, high blood flow state before chemotherapy and the rise in the average through time after chemotherapy illustrated that it was sensitive to chemotherapy, good curative effect, remission rate.%目的:探讨肺癌化疗前后灌注参数值的改变,评估CT灌注成像在肺癌化疗疗效评价中的价值。方法2013年1月至2013年12月选取符合标准的患者30例,分析其化疗效果和化疗前后CT灌注成像参数值的变化。结果30例患者完成2个周期化疗后PR 2例、CR 10例、SD 13例和PD 5例。化疗前有效组患者BF值高于无效组,差异有统计学意义(P0.05)。有效组患者化疗后BF值下降、MTT值升高,较治疗前差异均有统计学意义(P0.05)。无效组患者化疗前后BF、BV、MTT和PS值变化不大,差异无统计学意义( P>0.05)。结论 CT灌注成像能够反映非小细胞肺癌的血供情况,化疗前高血流量状态及化疗后平均通过时间升高提示其对化疗反应相对敏感,近期疗效好,缓解率高。

  10. 卡培他滨单药治疗老年晚期乳腺癌的临床观察%Clinical Observation of Capecitabine Monotherapy in Treatment of Elder Patients with Advanced Breast Cancer

    Institute of Scientific and Technical Information of China (English)

    方立萍; 蒋葵; 吴紫权; 张阳

    2012-01-01

    目的 观察卡培他滨单药治疗老年晚期乳腺癌的临床疗效和毒性反应.方法 36例Ⅳ期老年乳腺癌患者均经病理组织学或细胞学检查确诊.治疗剂量卡培他滨:2500 mg/m2,分早晚2次餐后半小时口服,第1 ~ 14天,每3~4周重复,2周期后评价疗效.结果 36例患者均可评价,其中获完全缓解(CR)0例,部分缓解(PR) 10例,稳定(SD) 14例,进展(PD)12例,总有效率27.8% (10/36).临床获益率(CR+ PR+ SD) 66.7% (24/36),中位疾病进展时间(TTP)7.1个月.主要的毒副反应为手足综合症、腹泻、口腔炎,均可耐受,无化疗相关死亡.结论 卡培他滨单药治疗老年晚期乳腺癌疗效确切,可明显改善患者生存质量,延长生存时间,副反应轻,耐受性良好并适合门诊治疗,是一种安全有效的姑息治疗方案.%Objective To evaluate the clinical efficacy and toxicity of capecitabine monotherapy in treatment of elder patients with advanced breast cancer. Methods Thirty six patients with advanced breast cancer confirmed by pathology or cytology were enrolled into the study. The patients received capecitabine 1250 mg/m2 twice daily,within 30 min after meal dl -14,every 3 -4 weeks and were evaluated efficacy after 2 cycles. Results None complete remission( CR) case was observed,PR was in 10 cases, 14 cases had stable disease( SD) and 12 cases got progressive disease( PD). The clinical benefit response rate( CR + PR + SD) was 66.7% (24/36). The median time to progression(TTP) was 7. 1 months. The main side effects were hand-foot syndrome , diarrhea and stomatitis which were all tolerable. Conclusion The regimen of capecitabine is effect, safe and well-tolerable in the treatment of elder patients with advanced breast cancer. It can significantly improve the quality of life in patients with advanced breast cancer, and prolong the survival time. The side effects are light and were well tolerated. It is suitable for outpatient treatment. The method is a safe

  11. Docetaxel plus Capecitabine Combination Therapy for Patients with Anthracycline-resistant Metastatic Breast Cancer%多西紫杉醇联合卡培他滨治疗蒽环类耐药的晚期乳腺癌

    Institute of Scientific and Technical Information of China (English)

    柴文英; 马芸; 李萍; 钱锦贤; 蒋爱梅

    2011-01-01

    目的:观察多西紫杉醇联合卡培他滨(DC)治疗蒽环类耐药晚期乳腺癌的疗效和安全性.方法:31例经病理证实的蒽环类耐药的转移性乳腺癌患者,采用多西紫杉醇联合卡培他滨化疗.剂量为多西紫杉醇75mg/m2,静滴,d1;希罗达2 500mg/m2,口服,d1~d14,21天为1个周期,化疗至疾病进展或不良反应无法耐受.根据WHO制定的实体瘤客观疗效评价标准和抗癌药物毒性分级标准评价疗效和不良反应.结果:31例患者共完成118个周期化疗,每例患者化疗2个~8个周期,中位周期数4个.31例患者中,完全缓解1例(3.2%),部分缓解16例(51.6%),稳定10例(32.3%),进展4例(12.9%),总有效率为54.8%.中位肿瘤进展时间6.3个月,中位生存期13个月,1年生存率为58.8 %.主要不良反应为骨髓抑制、胃肠道反应和手足综合征,患者均可耐受.结论:多西紫杉醇联合卡培他滨治疗蒽环类耐药晚期乳腺癌的疗效较好,不良反应可以耐受,是治疗蒽环类耐药晚期乳腺癌的有效方案.%Objective: To evaluate the efficacy and toxicity of docetaxel plus capecitabine in the treatment of anthracycline-resistant metastatic breast cancer. Methods: Thirty-one patients with anthracycline-resistant metastatic breast cancer were treated with chemotherapy combined with docetaxel and capecitabine. Docetaxel ( 75mg/m2 ) was given intravenously on d1 and capecitabine ( 2 500mg/m2 )was taken orally on d1 to d14, every 21 days as one cycle. Results: A total of 118 cycles of the regimen were administered in the 31 cases with a median of 4 cycles( range 2 ~ 8 cycles for each patient ). The overall response rate was 54.8%. Among the 31 patients, one got complete response( CR ), 16 partial response( PR ), 10 stable disease( SD ) and4 progressive disease( PD ). The median time of progression ( TTP ) was 6.3 months. The median survival time was 13 months. The one-year survival rate was 58.8%.The major toxicity and adverse effects

  12. Syntheses, Structures and Thermal Studies of Fluorinated and Non-Fluorinated Homo and Heterometallic Alkoxides

    Science.gov (United States)

    Samuels, John Andrew

    The following studies probe the effects of ligand structure and modification on the conversion of metal species to solid-state products. 1. The synthesis, characterization, thermal decomposition, and full hydrolysis of rm Cu^{I }_2 Zr_2(O^{i}Pr) _{10} (1), Cu^ {rm I}_4Zr_4 O(O^{rm i}Pr) _{18} (2), and Cu ^{rm II}_4Zr _4O_3(O^ {rm i}Pr)_{18 } (3) are reported. The structure of 1 contains a Zr_2(O^{rm i}Pr)_9^- face -sharing bioctahedron bound to a Cu^{ rm I}_2(mu_2-OR) ^+ fragment via two mu _2 -alkoxides. The structure for 2 contains two (C_2(mu_2-OR) _2Zr_2(mu_2 -OR)_3(OR)_4] ^{1-} fragments connected via a pseudo-tetrahedral mu_4-O ^{2-} ligand. The structure of 3 contains a planar Cu_4O(O ^{rm i}Pr)_2 ^{4+} fragment capped by two Zr _2O(O^{rm i}Pr)_8^{2-} face-sharing bioctahedral units. The central oxo of the copper fragment is square-planar. TGA studies of ClCuZr_2(O^{ rm i}Pr)_9, 1, 2, and 3 show that internal redox occurs, producing copper metal, acetone, and isopropanol. The final products contain an increasing amount of zirconia with increasing number of oxo ligands in the precursor species. Full hydrolysis of 1 and 3, followed by thermolysis, produces Cu ^0, Cu_2O, and CuO with zirconia, depending on thermolysis conditions. 2. The compounds Na(HFIP) (HFIP = OCH(CF _3)_2), 1a, Na(TFTB) (TFTB = OC(CH _3)_2(CF_3 )), 1b, Na(HFTB) (HFTB = OC(CH_3 )(CF_3)_2), 1c, Na(PFTB) (PFTB = OC(CF_3)_3), 1d, Zr(HFIP)_4, 2a, Zr(TFTB) _4, 2b, Zr(HFTB)_4, 2c, Zr(PFTB)_4, 2d, Na_2 Zr(OR_{rm f})_6 (C_6H_6)_ {rm n} (3a when n = 1 and 3b when n = 2), and Tl_2Zr(OR_ {rm f})_6, 4, have been synthesized and characterized by mass spectrometry, multinuclear NMR and (for 1a, 2c, 3a, 3b and 4) X-ray diffraction structure determinations. Intramolecular bonding interactions between organofluorines and sodium or thallium are seen in all structures and are supported by Tl/F coupling in ^{205}Tl and ^{19 }F NMR spectra. Chemical vapor depositions of 1a-d, 2b-d and 3 produce metal fluoride

  13. SMALL-DOSE CYTOKINES IN COMBINATION WITH 5-FLUOROURACIL IN OLISSEMINATED RENAL CELL CARCINOMA: FINAL RESULTS OF A RANDOMIZED TRIAL

    Directory of Open Access Journals (Sweden)

    L. V. Demidov

    2009-01-01

    Full Text Available Background: High and intermediate IL-2 regimens are difficult to recommend because of great toxicity and efficacy is not sufficient. We suggest that a combination of very low-dose cytokines is effective and safe in metastatic renal cell carcinoma (MRCC patients (pts. A prospective randomized study was started in 2003. The primary end-point was a response rate. Methods: The eligibility criteria included histopathologically confirmed MRCC, ECOG PS 0-2, no autoimmune diseases, no brain metastases, and normal organ function. All pts were randomized in three arms: IL-2 alone, 1.5 MIU, iv, t.i.w., weeks 1—3 or IL-2 1.0 MIU, iv, t.i.w., weeks 1—3 plus IFN 5 MIU, sc, t.i.w, weeks 1—3 or biochemotherapy group 5-FU, 500 mg/m2, iv, once a week, weeks 1—3 plus IL-2 1.0 MIU, iv, t.i.w., weeks 1—3 plus IFN 5 MIU, sc, t.i.w., weeks 1—3. Courses were repeated every three weeks. A response was assessed according to the RECIST every 2 courses.Results: 64 pts were enrolled, of whom 63 were analyzed. Their median age was 55.4 years (range 16—74. 42.9% of the patients had pre- viously received chemo- or immunotherapy. 55.6 percent of the pts had poor prognosis (according to Motzer et al., 2002. Bone metastases were present in 52.4% of the pts. Sixteen patients treated with IL-2 alone showed no CR, PR, 2 SD, or 14 PD. Of 23 patients in the IL-2+IFN group, there were 5 PR, 8 SD, and 10 PD, with a response rate of 21.7%. Amongst 24 patients in the 5-FU+IL-2+IFN group, there were 1 CR, 3 PR, 10 SD, and 10 PD, with a response rate of 16.7%. One-year survival was 20.0%, 81.3% and 81.0%, respectively. The influenza-like syndrome was the most common side effect in the pts who received IFN (89.1%, grade 1, CTC. Hypotension associated with IL-2 (all groups was seen in 56.3% (50%, grade 1 and 6.3%, grade 2. The other adverse reactions were 12.7% grade 1 neutropenia and vomiting in 4.7% pts (Group 3.Conclusion: All regimens are well tolerated. Small-dose IL-2

  14. Magnetic characterization of distal IRD layers at the NW Iberia Continental margin

    Science.gov (United States)

    Rey, D.; Mohamed, K. J.; Andrade, A.; Rodríguez-Germade, I.; Coimbra, R. L.; Rubio, B.; Bernabeu, A. M.; Alvarez-Iglesias, P.; Frederichs, T.

    2012-12-01

    during the Heinrich events. H-events in the study area have been assigned to those horizons that exceed 0.5 times the standard deviation background value recalculated to Ln(k) of a given core after detail magnetic characterization of "IRD-suspicious" layers. The robustness of the method have been demonstrated against increasing grain size trends loops on the ARM vs. K plots, the identification of larger size magnetite grains by low temperature techniques, EDX assisted BS-SEM, consistency with elemental FRX derived composition down-core trends, and 14C ages in hemipelagic layers. The method allowed fast recognition of events H1 to H5 at the study area and subsequently the creation of a robust and inexpensive geochronological framework. Further to this, we have been able to identify the presence of H-layer derived diagenetic greigite in some of the horizons. Bond, G. et al., 1992. Nature, 360, 245- 249. Heinrich, 1988. Quaternary Research, 29, 142-152 . Kissel, 2005. C. R. Geoscience 337, 908-918 Lebreiro, et al.,1996. Marine Geology 131, 47-56. Moreno et al. 2002. EPSL, 202, 465-480. Rey et al., 2008. Mar. Geol, 249, 64-92 . Robinson et al. 1995. Paleoceanography, 10, 221-250 . Ruddiman, W.F. (1977). Geol. Soc. Am. Bull. 88, 1-12 . Thouveny, et al. 2000. EPSL, 180, 61-75. Cont. Proj. CTM2007-61227/MAR, GCL2010-16688, 09MMA012312PR & 10MMA312022PR

  15. 多西他赛联合顺铂与培美曲塞联合顺铂治疗晚期肺腺癌的随机对照研究%Prospective randomized controlled clinical trial of docetaxel plus cisplatin versus pemetrexed plus cisplatin as treatment for advanced lung adenocarcinoma

    Institute of Scientific and Technical Information of China (English)

    邹三鹏; 薛静

    2016-01-01

    案(PP方案)一线治疗晚期肺腺癌的近期疗效和不良反应.方法 收集2012年8月至2014年8月在西安交通大学第一附属医院(46例)和广东医学院附属惠东医院(20例)收治的66例初治晚期肺腺癌患者,采用随机对照的临床研究设计,将66例经组织学或细胞学确诊的初治晚期肺腺癌患者分为DP方案组和PP方案组各33例.观察两组患者的近期有效率(RR)、疾病控制率(DCR)、疾病进展时间(TTP)、中位生存时间(MST)、1年生存率和不良反应.结果 DP方案组中,部分缓解(PR)10例,疾病稳定(SD)15例,疾病进展(PD)8例,RR为30.30%(10/33),DCR为75.76%(25/33);TTP为7.8个月,MST为13.5个月,1年生存率为54.55%(18/33).PP方案组中,PR 11例,SD 16例,PD 6例,RR为33.33%(11/33),DCR为81.82%(27/33),TTP为8.1个月,MST为14.8个月,1年生存率为57.58%(19/33).两组患者的近期疗效和生存率比较差异均无统计学意义(P≥0.05).两组患者的主要不良反应为血液学毒性、消化道反应和脱发.PP组的不良反应中中性粒细胞减少、血小板减少及脱发的发生率明显低于DP组,差异具有统计学意义(P<0.05).结论 DP方案和PP方案治疗晚期肺腺癌疗效确切,不良反应均可耐受.

  16. 恶性腹腔积液体外高频深部热疗联合腹腔化疗的临床观察%Clinical research of external high frequency hyperthermia plus perfusion chemotherapy on malignant peritoneal effusion

    Institute of Scientific and Technical Information of China (English)

    李海金; 董良; 陈亚男; 李英; 尚官敏; 葛丽娜; 赵建刚; 王伟英; 方萍

    2011-01-01

    high frequency hyperthermia plus intraperitoneal chemotherapy on malignant peritoneal effusion is clearly superior to that of intraperitoneal chemotherapy alone, and the toxicity is also reduced.%目的:观察体外高频深部热疗联合腹腔化疗治疗恶性腹腔积液的近期疗效和不良反应.方法:70例患者随机分为体外高频深部热疗联合腹腔化疗组(治疗组)35例和单用腹腔化疗组(对照组)35例,均采用中心静脉导管腹腔内置管引流,引流量1 000~2 000 mL/d,需3~5 d,最大限度引尽腹腔积液后经中心静脉导管注入分别含有顺铂40 mg/m2、5-氟尿嘧啶750 mg/m2、地塞米松10 mg的生理盐水1 500~2 000 mL,肝素帽封管,嘱患者每10 min变换体位1次使药物在腹腔内分布均匀.治疗组于给药后30 min开始腹部体外高频深部热疗,设定治疗温度43℃,持续1h,对照组单用腹腔化疗,两组均每周1次共3次,然后评定近期疗效,观察不良反应.结果:治疗组完全缓解(CR)23例,部分缓解(PR)8例,有效率(RR)88.58%;对照组CR 12例,PR 10例,RR62.86%;两组比较差异有统计学意义,x2 =6.29,P=0.01.Kamofsky评分改善分别是27例(77.14%)和18例(51.43%),两组比较差异有统计学意义,x2=5.04,P=0.02.不良反应均能耐受,两组比较差异无统计学意义,P>0.05.结论:体外高频深部热疗联合腹腔化疗治疗恶性腹腔积液疗效明显提高,不良反应减轻.

  17. 多西他赛联合卡培他滨治疗复发转移乳腺癌的疗效及相关因素研究%Clinical study of low-dose docetaxel combined with capecitabine for metastatic breast cancer

    Institute of Scientific and Technical Information of China (English)

    陆海林; 李燕; 宁志强; 曹婷华; 陈超

    2011-01-01

    were evaluable for efficacy. The overall response rate was 55. 3% (21/38), with 3 patients of CR, 18 patients of PR, 10 patients of SD and 7 patients of PD. The median PFS of 38 patients was 7. 1 months. The most frequent treatment-related adverse events were nausea/vomiting, fatigue, leukopenia and hand-foot syndrome. Among them, grade 3-4 leukopenia was 15. 8% and no treatment-related deaths occurred. PS(P=0.003), pausimenia status(P= 0.043) and number of transfer(P=0.021) were related to RR, while only PS was the independent prognostic factor (P < 0. 05). PS was the only independent prognostic factor for PFS by Cox proportion hazard model ( P < 0.05). Conclusion Low-dose docetaxel combined with capecitabine is effective and safe in the treatment of metastatic breast cancer and adverse events were tolerable. PS is the only independent prognostic factor for efficacy of XT regimen in metastatic breast cancer.

  18. A Controlled Clinical Study between Hepatic Arterial Infusion with Embolized Curcuma Aromatic Oil and Chemical Drugs in Treating Primary Liver Cancer%莪术油和化疗药对照肝动脉灌注栓塞治疗原发性肝癌的临床研究

    Institute of Scientific and Technical Information of China (English)

    程剑华; 常纲; 吴万垠; 刘伟胜; 杨志钢; 孟凡喆; 徐凯; 李柳宁; 朱迪盈; 陈春泳; 罗海英

    2001-01-01

    均按辨证施治同时服用中药。结果:治疗组CR1例,PR13例,总有效率为43.75%(14/32);甲胎球蛋白(AFP)转阴7例,滴度下降7例。化疗组PR10例,有效率为31.25%(10/32);AFP转阴5例,滴度下降2例,两组疗效比较差异无显著性(P>0.05)。治疗后出现灌注栓塞综合征(发热、腹痛、呕吐等)两组相似;但治疗组无明显骨髓抑制现象,两组比较差异有显著性(P<0.01或P<0.05)。治疗组平均生存期11.5个月,中位生存期10个月;1、2、3年生存率分别为37.5%(12/32)、13.3%(4/30)、6.9%(2/29)。化疗组平均生存期7.25个月,中位生存期6个月;1、2、3年生存率分别为15.6%(5/32)、3.2%(1/31)、0。治疗组平均生存期、中位生存期、1年生存率均明显优于化疗组(P<0.05)。结论:莪术油肝动脉灌注栓塞治疗原发性肝癌疗效与化疗药相似,但生存时间和骨髓抑制明显优于化疗药。

  19. 利用酵母双杂交筛选与苹果褪绿叶斑病毒CP互作的寄主因子%Screening of the Host Factors Interacting with CP ofApple chlorotic leaf spot virusby Yeast Two-Hybrid System

    Institute of Scientific and Technical Information of China (English)

    何乙坤; 钟敏; 胡同乐; 王树桐; 段豪; 丁丽; 王亚南; 曹克强

    2014-01-01

    -1,Malus4-2,Malus4q1,Malus31-3,Malus49q2,Malus15-1,Malus6-2,and Malus91-4) may play a great role in the interaction between host and virus.[Conclusion]According to the results, the function of the important proteins was analyzed. It was presumed that ACLSV CP interacting with BZIP, MYB transcription factors, pathogenesis-related protein (PR-5, PR-8, PR-10), which may regulate the host resistance to virus. CP interacting with photosystem II stability/assembly factor protein and iron-binding protein may affect the stability of photosystem and the formation of chlorophyll, thereby reducing photosynthesis. This will provide a theoretical basis for revealing the mechanism of chlorotic green leaf spot and tree decline caused by ACLSV.%目的利用酵母双杂交系统筛选与苹果褪绿叶斑病毒(Apple chlorotic leaf spot virus, ACLSV)外壳蛋白(coat protein, CP)互作的寄主因子,利用生物信息学方法分析其在病毒与寄主互作过程中可能发挥的作用。方法首先构建ACLSVCP的酵母双杂交诱饵载体pGBKT7-CP,转化至酵母菌株Y2H gold中,测定转化菌在SD/-Trp、SD/-Trp/-His和SD/-Trp/-Ade-X-α-Gal平板上的生长情况,从而判断其在酵母双杂交过程中对报告基因的自激活活性。将pGBKT7-CP及pGBKT7分别转化至酵母菌株Y2H gold,培养不同时间后,用紫外分光光度计测定OD 600值,分析pGBKT7-CP对酵母菌生长的影响;然后通过顺序转化的方法自前期已构建好的苏俄苹果(Malus sylvestris cv. R12740-7A)cDNA文库中钓取与ACLSV CP互作的因子,最终在SD/-Ade/-His/-Leu/-Trp/X-α-Gal平板上筛选生长良好的蓝色阳性菌落,测定序列,并在GenBank中对其进行BLAST分析,根据基因注释推断其在病毒与寄主互作过程中可能发挥的作用。结果成功构建了酵母双杂交诱饵载体pGBKT7-CP,转化至酵母菌株Y2H gold后,转化菌只能在SD/-Trp平板上正常生长,不能在SD/-Trp/-His和SD/-Trp/-Ade

  20. ESO PR Highlights in 2006

    Science.gov (United States)

    2007-01-01

    Last year proved to be another exceptional year for the European organisation for ground-based astronomy. ESO should begin the New Year with two new member states: Spain (PR 05/06) and the Czech Republic (PR 52/06). ESO PR Highlights 2006 2006 was a year of renovation and revolution in the world of planets. A new Earth-like exoplanet has been discovered (PR 03/06) using a network of telescopes from all over the world (including the Danish 1.54-m one at ESO La Silla). It is not the only child of this fruitful year: thanks to the combined use of ESO's Very Large Telescope (VLT) and La Silla instruments, a surprising system of twin giant exoplanets was found (PR 29/06), and a trio of Neptune-like planets hosted by a nearby star were identified (PR 18/06). These results open new perspectives on the search for habitable zones and on the understanding of the mechanism of planet formation. The VISIR instrument on the VLT has been providing unique information to answer this last question, by supplying a high resolution view of a planet-forming disc (PR 36/06). There are not only new members in the planets' register: during the General Assembly of the International Astronomical Union held in Prague (Czech Republic), it was decided that Pluto is not a planet anymore but a 'dwarf planet'. Whatever its status, Pluto still has a satellite, Charon, whose radius and density have been measured more accurately by observing a rare occultation from different sites, including Cerro Paranal (PR 02/06). The scientific community dedicated 2006 to the great physicist James Clerk Maxwell (it was the 175th anniversary of the birth): without his electromagnetic theory of light, none of the astonishing discoveries of modern physics could have been achieved. Nowadays we can look at distant galaxies in great detail: the GIRAFFE spectrograph on the VLT revealed that galaxies 6 billion years ago had the same amount of dark matter relative to stars than nowadays (PR 10/06), while SINFONI gave an

  1. Consumo precoz de tabaco y alcohol como factores modificadores del riesgo de uso de marihuana Early tobacco and alcohol consumption as modifying risk factors on marijuana use

    Directory of Open Access Journals (Sweden)

    Verónica Iglesias

    2007-08-01

    idade de início de consumo de tabaco e/ou álcool e a intensidade de uso de tabaco. Uso de maconha e idade de início do consumo foram as variáveis de desfecho. Para a análise dos dados se utilizou regressão de Poisson e regressão de Weibull. RESULTADOS: Os escolares apresentaram alta prevalência de consumo de tabaco, álcool maconha (77%, 79% e 23%, respectivamente. O consumo de tabaco na presença de consumo de álcool foi um fator de risco de uso de maconha (RP=10,4; IC 95%: 8,9;12,2. O início tardio de consumo de tabaco (HR=0,85; IC 95%: 0,84;0,86 e álcool (HR=0,90; IC 95%: 0,89;0,91 resultou ser um fator protetor do uso de maconha. A probabilidade de consumo de maconha foi maior naqueles que fumavam diariamente ou quase, em relação àqueles que fumavam somente aos fins de semana (RP=3,11; IC 95%: 2,96;3,26 vs. RP=1,70; IC 95%: 1,58;1,83. CONCLUSÕES: O risco de consumo de maconha se associou significativamente à idade de início de consumo de tabaco, à freqüência de consumo de tabaco e ao consumo simultâneo de álcool. As estratégias de prevenção deveriam proteger os escolares do consumo precoce de tabaco.OBJECTIVE: To assess the relationship between early tobacco and alcohol use and the risk of marijuana consumption among schoolchildren. METHODS: A cross-sectional study comprising data from the Fourth National Study on Drug Use in the Chilean School Population (2001. There were studied 54,001 schoolchildren aged between eight and 20 years. Predictors were self-reported tobacco and alcohol use (ever users, age at initiation of tobacco and/or alcohol use, and intensity of tobacco use. The study outcome was self-reported marijuana use (ever users versus never users and age at initiation of marijuana use. Poisson regression and Weibull regression were used for data analysis. RESULTS: Lifetime prevalence of tobacco use was high: 77%, alcohol 79%, and marijuana 23%. Tobacco consumption increased the likelihood of marijuana use (PR=10.4; 95% CI: 8

  2. Three Good Reasons for Celebrating at the ESO/ST-ECF Science Archive Facility

    Science.gov (United States)

    2000-12-01

    Great Demand for Data from New "Virtual Observatory" Summary Due to a happy coincidence, the ESO/ST-ECF Science Archive Facility is celebrating three different milestones at the same time: * its 10th anniversary since the establishment in 1991 * the 10,000th request for data , and * the signing-up of active user number 2000 . This Archive contains over 8 Terabytes (1 Terabyte = 1 million million bytes) of valuable observational data from the NASA/ESA Hubble Space Telescope (HST), the ESO Very Large Telescope (VLT) and other ESO telescopes . Its success paves the way for the establishment of "Virtual Observatories" from which first-class data can be obtained by astronomers all over the world. This greatly enhances the opportunities for more (young) scientists to participate in front-line research. PR Photo 34/00 : Front-page of a new brochure, describing the ESO/ST-ECF Science Archive Facility. Just 10 years ago, on the 1st of January 1991, the ESO/ST-ECF (European Southern Observatory/Space Telescope-European Coordinating Facility) Science Archive Facility opened. It has since served the astronomical community with gigabyte after gigabyte of high-quality astronomical data from some of the world's leading telescopes. The Archive, which is located in Garching, just outside Munich (Germany), contains data from the 2.4-m NASA/ESA Hubble Space Telescope , as well as from several ESO telescopes: the four 8.2-m Unit Telescopes of the Very Large Telescope (VLT) at the Paranal Observatory , and the 3.5-m New Technology Telescope (NTT) , the 3.6-m telescope and the MPG/ESO 2.2-m telescope at La Silla. The Archive is a continuously developing project - in terms of amounts of data stored, the number of users and in particular because of the current dramatic development of innovative techniques for data handling and storage. In the year 2000 more than 2 Terabytes (2000 Gigabytes) of data were distributed to users worldwide. The archiving of VLT data has been described in ESO PR

  3. Weight development and survival rate of reindeer calves treated against Oedemagena tarandi L and Cephenemyia trompe L

    Directory of Open Access Journals (Sweden)

    Endre Persen

    1982-05-01

    aktivt stoff pr. 10 kg levende vekt. I alt inngår 911 kalver og 403 voksne simler i forsøkene. Forsøkene har søkt å gi svar på mulige effekter av nevnte behandling på a vektutvikling om vinteren, b vektutvikling frem til normalt slaktetidspunkt (18 mndr.,c tap av dyr om vinteren. Videre er det søkt å gi svar på om behandlingstidspunktet (27/11 og 14/2 har betydning for vekttapet om vinteren, alle undersøkelser er gjort innen kjønn. Generelt viser forsøkene at behandlede dyr tar av mer i vekt om vinteren enn ubehandlede dyr. Det ble funnet signifikant større vekttap for behandlede simlekalver (0.7 kg, p=0.005 i tidsrommet 13/1—28/4 i forsøk I og for behandlede bukkekalver (0.8 kg, p=0.01 i tidsrommet 14/2—4/4 i forsøk II. Det er også en tendens til at behandlingen virker til å øke slaktevekten. Det ble således funnet signifikant høyere økning i slaktevekt (0.8 kg, p=0.05 for behandlede bukker i forsøk III. Det ble ikke påvist effekt av behandlingen på tap av dyr om vinteren. De kalvene som ble borte om vinteren hadde signifikant lavere vekt ved forsøkets start sammenlignet med de som overlevde. I perioden 14/2—4/4 ble det funnet større vekttap hos de bukkekalver som ble behandlet 14/2 sammenlignet med behandling 27/11 (0.5 kg, p=0.01.Koikelu poronvasojen hoitamiseksi poropaarmantoukkia vastaan. Vaikutus vasojen kuntoon mittana elopaino ja henkiinjååmiskyky.Abstract in Finnish / Yhteenveto: Suoritettiin kolme koetta porossa loisina esiintyvien kurmunja saulakantoukkien (Oedemagena tarandi L ja Cephenemyia trompe L torjumiseksi. Kokeissa kåytetiin Warbex-35%:sta valmistetta ruiskeena lihakseen, annoksen suuruus oli 30 mg vaikuttavaa ainetta 10 elopainokiloa kohti. Kaikkiaan kokeissa kåsiteltiin 911 vasaa ja 403 vaadinta. Kokeissa selviteltiin kåsittelyn mahdollisia seurausvaikutuksia a talven aikaiseen painonkehitykseen b painonkehitykseen normaaliin teurastusajankohtaan saakka (18 kk c elainten talviaikaiseen katoon. Lisåksi pyrittiin

  4. Missing Black Holes Driven Out

    Science.gov (United States)

    2004-05-01

    Active galaxies Active galaxies are breathtaking objects. Their compact nuclei (AGN = Active Galaxy Nuclei) are so luminous that they can outshine the entire galaxy; "quasars" constitute extreme cases of this phenomenon, their powerful engine making them visible over a very large fraction of the observable Universe. It is now widely accepted that the ultimate power station of these activities originates in supermassive black holes with masses up to thousands of millions times the mass of our Sun, cf. e.g., ESO PR 04/01. For comparison, the one in the Milky Way galaxy has only about 3 million solar masses, cf. ESO PR 17/02. The central black hole is believed to be fed from a tightly wound accretion disc of gas and dust encircling it, in a donuts-shaped torus (cf. ESO PR 10/04). Material that falls towards these gigantic "vacuum cleaners" will be compressed and heated up to enormous temperatures. This hot gas radiates an incredible amount of light, causing the active galaxy nucleus to shine so brightly. Because of this obscuring dust torus, the aspect of the AGN or the quasar may greatly vary. Sometimes, astronomers can look along the axis of the dust torus from above or from below and thus have a clear view of the black hole. Such objects are called "Type-1 sources". "Type-2 sources", however, are oriented such that the dust torus is seen edge-on from Earth, and our view of the black hole is therefore totally blocked by the dust over a large range of wavelengths from the near-infrared to soft X-rays. Type-2 quasars - where are they? While many examples of rather close-by Type-2 AGNs are known (so-called Seyfert 2 galaxies), it is still a matter of debate whether their larger luminosity quasar counterparts exist. Until very recently, very few examples of this class were known. One of them is the Type-2 Quasar CXOCDFS J033229.9-275106, discovered by combining observations taken in X-rays with spectra obtained by the Very Large Telescope (ESO PR 05/01). It is indeed a

  5. Young and Waltzing Binary Stars

    Science.gov (United States)

    2001-10-01

    current ESO-ESA CERN educational programme on "Life in the Universe". [3] In the case of exoplanets, the planet itself is not visible, but the spectral lines from the star are seen to wobble due to the gravitational influence of the planet, cf. ESO PR 07/01. [4] Several ESO Press Releases concern observations of the element Lithium in stars, e.g., PR 03/99 (in a giant star), PR 08/00 (in a metal-poor star) and PR 10/01 (from a "swallowed" exoplanet).