WorldWideScience

Sample records for adn hiv-t adn

  1. Patentando ADN

    Directory of Open Access Journals (Sweden)

    Carlos Augusto Conde Gutiérrez

    2011-11-01

    Full Text Available Los sistemas de patentes en los países desarrollados han hecho diferentes interpretaciones o creado nuevos estándares con el fin de adoptar secuencias de ADN como una entidad patentable, independientemente del hecho de que el ADN en principio no cumplía los requisitos para obtener una patente (novedad, nivel inventivo y aplicación industrial –utilidad– y materia objeto de patentes. Este enfoque benefició I&D en la industria de la biotecnología. Sin embargo, las patentes representan también una amenaza para I&D. Este artículo analiza cómo los sistemas de patentes en Europa adoptaron las secuencias de ADN y cómo podría afectar a las patentes I&D en biotecnología.

  2. Analyzing ADN competencies.

    Science.gov (United States)

    Mitchell, P R

    1989-01-01

    El Paso Community College District, using the DACUM Process, identified 19 major competency areas with 313 specific competencies for AD Nursing. This article provides an overview of the DACUM Process, a discussion of the application to the ADN program, a summary of the results, and future activities.

  3. La prueba de ADN

    OpenAIRE

    Carro Fernández, José Roberto

    2015-01-01

    Podríamos decir sin riesgo a equivocarnos que, en España, el momento procesal penal en lo tocante a la prueba de ADN pasa por un momento de crisis. Y no en lo referente a su valor probatorio, pues éste está fuera de toda duda y así lo estableció el mismo Tribunal Supremo el 24 de febrero de 1995 al considerar que “la prueba de ADN no es técnicamente determinante, pero sí de alta probabilidad”. Ahora bien, esta validez jurídica no resuelve otros aspectos como podrían ser si la toma de la muest...

  4. Assimilation Dynamic Network (ADN) Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Assimilation Dynamic Network (ADN) is a dynamic inter-processor communication network that spans heterogeneous processor architectures, unifying components,...

  5. Marcadores virológicos no convencionales en pacientes infectados con el virus de la inmunodeficiencia humana: ADN HIV-T, ADN HIV- 2LTR y ARN de HIV Non conventional virological markers in HIV-infected patients: T-HIV DNA, 2LTR-HIV DNA and HIV RNA

    Directory of Open Access Journals (Sweden)

    Rosana Gariglio

    2004-10-01

    Full Text Available La terapia antirretroviral de alta eficacia (TAAE induce una reducción marcada y persistente de la viremia plasmática, contribuyendo a disminuir la mortalidad y morbilidad de los pacientes HIV-positivos. Así, la carga viral (CV es el método de referencia para evaluar la eficacia terapéutica. Sin embargo, aun en presencia de una TAAE eficiente no se ha logrado la erradicación viral. En este estudio analizamos la presencia del ADN total de HIV (ADN HIV-T, del ADN no integrado con 2LTR (ADN HIV-2LTR y del ARN de HIV, en un grupo de 55 pacientes HIV-positivos en distintos estadios clínicos, con y sin TAAE, mediante ensayos de PCR con revelado colorimétrico en microplaca, optimizados en nuestro laboratorio. La sensibilidad clínica del ARN del HIV fue evaluada con el bDNA, resultando del 74% y del 64%, respectivamente, con una concordancia del 85%. Este ensayo podría ser utilizado en el seguimiento de pacientes bajo TAAE. El ADN HIV-2LTR resultó positivo en el 54% aunque estuvo ausente en pacientes con elevada CV. Este marcador se consideraba un producto lábil y su presencia se asociaba a infección reciente. Sin embargo, actuales evidencias ponen en discusión su estabilidad por lo que su significado clínico debe ser reconsiderado. La ausencia del ADN HIV-2LTR en pacientes con CV detectable puede relacionarse con la heterogeneidad de la secuencia utilizada para su detección. El ADN HIV-T estuvo presente en el 100% de las muestras y resultaría relevante como marcador de remisión cuando se dispongan de terapias que efectivamente erradiquen la infección.Highly active antiretroviral therapy (HAART induces a persistent reduction of the plasmatic viremia, contributing to decrease mortality and morbidity of infected people with human immunodeficiency virus (HIV. Thus, viral load (VL is the reference method to evaluate therapy effectiveness. However, even in the presence of efficient HAART viral eradication was yet not achieved. In this

  6. Comparison of evidenced and expected ADN competencies.

    Science.gov (United States)

    Burch, S; Joyce-Nagata, B; Reeb, R

    1991-01-01

    These study findings indicate that nursing service administrators in the State of Mississippi expect strong technical level skills from the ADN. Congruency between nursing education and nursing service was validated. The predominance of role competencies outlined by nurse educators were validated as both expected and evidenced for the ADN in the State of Mississippi. Competencies need to be continually evaluated to reflect changes in the health delivery system as related to the ADN.

  7. DAO Spectroscopic classification of SN 2016adn

    Science.gov (United States)

    Balam, D. D.; Graham, M. L.

    2016-02-01

    A spectrum was obtained of AT2016adn on UT Feb. 11.30 UT using the 1.82-m Plaskett telescope (National Research Council of Canada) covering the range 380-910 nm (resolution 0.51 nm). Cross-correlation with a template library using SNID (Blondin & Tonry 2007, ApJ, 666, 1024) shows 2016adn to be a normal type-Ia supernova approximately 2 days post maximum light.

  8. The LPN-ADN articulation project.

    Science.gov (United States)

    Perlich, L J; Hopkin, L A; Kalunian, K; LeGault, G; Fried, M

    1989-01-01

    Faced with a staffing situation where RN positions were needed but there were more LPN positions than were needed. Tucson Medical Center began an LPN-ADN articulation project to rectify the situation without resorting to layoffs. With the cooperation of a local junior college, LPNs are enrolled in ADN programs that provide a more flexible work/study situation. This article describes the program and the results thus far.

  9. Detonation velocity of melt-cast ADN and ADN/nano-diamond cylinders

    Science.gov (United States)

    Doherty, R. M.; Forbes, J. W.; Lawrence, G. W.; Deiter, J. S.; Baker, R. N.; Ashwell, K. D.; Sutherland, G. T.

    2000-04-01

    Detonation velocities of confined cylinders of melt-cast ADN/ZnO (99.5/0.5 by weight), ADN/nano-diamond/ZnO (92.4/7.2/0.4), ADN/AN/ZnO (95.5/4.0/0.5) and ADN/AN/ZnO/nano-diamond (88.0/4.5/0.5/7.0) have been measured using a streak camera. Velocities ranging between 3.9 and 4.5 mm/μs were obtained for 1.3 cm diameter samples confined by steel and a 2.5 cm diameter ADN/AN/ZnO cylinder. In one of the samples the detonation was failing as it proceeded through the charge. For the other shots reported, the shock velocities appeared to be steady through the last half of the charge, though the lengths were too short for any definitive statement about the failure diameter to be made.

  10. Improving Clinical Teaching: The ADN Experience. Pathways to Practice.

    Science.gov (United States)

    Haase, Patricia T.; And Others

    Three Florida associate degree in nursing (ADN) demonstration projects of the Nursing Curriculum Project (NCP) are described, and the history of the ADN program and current controversies are reviewed. In 1976, the NCP of the Southern Regional Education Board issued basic assumptions about the role of the ADN graduate, relating them to client…

  11. New synthetic method and properties of ammonium dinitramide (ADN)

    Energy Technology Data Exchange (ETDEWEB)

    Hatano, H.; Onda, T.; Shi Ino, K.; Kiname, S.I. [Technical Center, Hosoya Kako, Osawa (Japan); Miyazaki, S.; Suzuki, S. [Research et Development Center, Nissan Motor Co, Saitama (Japan)

    1996-12-31

    Ammonium dinitramide (ADN) is one of the energetic materials, its hazard, however, has never been presented yet. In this paper, new synthetic method of ADN and its properties are contained. It has been found out that ADN has little hazard and is a safe energetic compound. (authors) 4 refs.

  12. Detonation Properties of Ammonium Dinitramide (ADN)

    Science.gov (United States)

    Wätterstam, A.; Östmark, H.; Helte, A.; Karlsson, S.

    1999-06-01

    Ammonium Dinitramide, ADN, has a potential as an oxidizer for underwater high explosives. Pure ADN has a large reaction-zone length and shows a strong non-ideal behaviour. The work presented here is an extension of previous work.(Sensitivity and Performance Characterization of Ammonium Dinitramide (ADN). Presented at 11th International Detonation Symposium, Snowmass, CO, 1998.) Experiments for determining the detonation velocity as a function of inverse charge radius and density, reaction-zone length and curvature, and the detonation pressure are presented. Measurements of pressure indicates that no, or weak von-Neumann spike exists, suggesting an immediate chemical decomposition. Experimental data are compared with predicted using thermochemical codes and ZND-theory.

  13. Formulation and Characterization of ADN-Based Liquid Monopropellants

    Science.gov (United States)

    Wingborg, N.; Eldsäter, C.; Skifs, H.

    2004-10-01

    Ternary ionic solutions are promising green propellants to replace monopropellant hydrazine. Ammonium dinitramide, ADN, is well suited as oxidizer in these propellants due to its high solubility. This paper presents the formulation of different ADN-based liquid monopropellants and the characterization of their properties such as stability, density, viscosity and sensitivity. To be able to use ADN-based monopropellants for propulsion applications, ADN must be produced in a way to minimize the effect on the environment and in sufficient quantities. This paper thus also briefly presents the industrial production of ADN in Sweden and the efforts made to optimize the process.

  14. The Properties of Ammonium Dinitramine (ADN): Part 2: Melt Casting

    Science.gov (United States)

    Hahma, A.; Edvinsson, H.; Östmark, H.

    2010-04-01

    A melt casting technique for ammonium dinitramine (ADN) and ADN/aluminum was developed. ADN proved relatively easy to cast, when 1% of magnesium oxide was used as a stabilizer and crystallization kernels. Densities of ADN/MgO 99/1 were 92 to 97% of theoretical mean density (TMD) and those of ADN/Al/MgO 64/35/1 were between 95 and 99% of TMD. Sedimentation of Al in the melt was prevented and the particle wetting was ensured by selecting a suitable particle size for Al. No gelling agents or other additives were used. The casting process and factors influencing it are discussed.

  15. Introducing ADN students to nursing research.

    Science.gov (United States)

    Thorpe, R; Smutko, P W

    1998-01-01

    Every nurse, regardless of educational preparation, should be involved in and benefit from nursing research. The research process needs to become an integral part of nursing practice. In this article, the authors emphasize the importance of nursing research in the associate degree nursing curriculum, emphasizing strategies that enable the ADN graduate to appreciate research reports and use the knowledge in the clinical practice setting.

  16. LPN/ADN Bridge Course. Instructor's Guide.

    Science.gov (United States)

    Barrett, Jan; And Others

    This curriculum guide has been constructed in order to bridge the skills gap between licensed practical nursing (LPN) programs and associate degree nursing (ADN) programs in Missouri. The curriculum was developed through a cooperative effort by persons from both sectors. The curriculum contains four major units, each consisting of several lessons,…

  17. A.D.N. Education: A Historical Perspective.

    Science.gov (United States)

    Moore, Anne Joachim

    Events in the history of Associate Degree Nursing (ADN) are summarized and the future of nursing education is projected in this paper. The establishment of the ADN program at St. Mary's Junior College in 1964 is considered first, with respect to its roots in the diploma program of St. Mary's School of Nursing and the ideas on nursing education of…

  18. ADN to BSN: lessons from human capital theory.

    Science.gov (United States)

    Graf, Christina M

    2006-01-01

    Currently, approximately 16% of associate degree nursing (ADN) graduates acquire baccalaureate or higher degrees. Human capital analysis demonstrated negative to minimal average returns on investment (ROI) in BSN education. Increasing the ROI may influence ADNs to pursue baccalaureate education and can be an effective strategy for meeting the projected need for BSN-prepared nurses.

  19. Cincuenta años de ADN

    OpenAIRE

    García Olmedo, Francisco

    2003-01-01

    Hace medio siglo que a James D. Watson y Francis Crick les tocó esa lotería que es la fama científica, una notoriedad que trasciende los estrechos límites de cualquier disciplina. Decir esto no supone desdeñar el indudable mérito de estos científicos al dilucidar la estructura tridimensional del ADN por métodos heterodoxos sino que intenta subrayar el hecho de que la estructura encontrada reunía unas características tales que su descubrimiento quedó singularizado frente a otros de igual o...

  20. Adsorption of ammonium dinitramide (ADN) from aqueous solutions. 1. Adsorption on powdered activated charcoal.

    Science.gov (United States)

    Santhosh, G; Venkatachalam, S; Ninan, K N; Sadhana, R; Alwan, S; Abarna, V; Joseph, M A

    2003-03-17

    Investigations on the adsorption of ammonium dinitramide (NH(4)N(NO(2))(2)) (ADN) from aqueous solutions on powdered activated charcoal (PAC) were carried out in order to find out an effective and easier method of separating ADN from aqueous solutions. The effectiveness of PAC in the selective adsorption of ADN from aqueous solutions of ADN (ADN-F) and ADN in presence of sulfate (SO(4)(2-)) and nitrate (NO(3)(-)) ions (ADN-PS) was examined and compared using batch and column methods. The adsorption process follows both Langmuir and Freundlich adsorption isotherms and the isotherm parameters for the models were determined. The observed data favor the formation of monolayer adsorption. The adsorption capacities were found to be 63.3, 119, 105.3 and 82 mg of ADN per g of PAC for ADN-F (batch), ADN-PS (batch), ADN-F (column) and ADN-PS (column), respectively. Break-through curves for ADN-F and ADN-PS were obtained for the optimization of separation of ADN from aqueous solutions. Elution curves were generated for the desorption of ADN from PAC using hot water as eluent.

  1. Differences in clinical experiences of ADN and BSN students.

    Science.gov (United States)

    Oermann, M H

    1998-05-01

    Prior research has suggested that clinical experience for nursing students is stressful. Concern about making an error and harming the patient, limited knowledge and skills for practice, and difficulties in interacting with the teacher and others in the clinical setting are some of the stressors reported by students. Few studies have compared these stresses and the clinical experiences in general between students in associate degree (ADN) and baccalaureate (BSN) nursing programs. The purposes of this research were to compare the clinical experiences of ADN and BSN students at different levels in the programs and describe these experiences from the students' perspectives. As such, both quantitative and qualitative data were collected from 415 students in ADN and BSN programs in the Midwest. The ADN students reported significantly higher stress in clinical practice than BSN students (t = 2.16, p ADN and BSN students in clinical practice increased as they progressed through the programs. The semester prior to graduation was the most stressful time in terms of clinical practice for both ADN and BSN students. The instructor was the predominant stressor reported by students in ADN programs across all levels of the curriculum. Among BSN students, the most prevalent stresses were coping with demands associated with patient care and the clinical teacher. The findings highlight the important role of the clinical faculty in both types of nursing programs.

  2. Fundamentos de computación con ADN

    Directory of Open Access Journals (Sweden)

    Jorge Eduardo Ortíz Triviño

    2011-01-01

    Full Text Available Este artículo presenta algunas ideas básicas para resolver problemas computacionales complejos, mediante el empleo de la estructura biológica del ADN y técnicas especializadas de laboratorio. Las características del ADN que se describen hacen posible codificar estructuras matemáticas, como las máquinas de Turing o las redes neuronales; para ello, la clave está en la interpretación del ADN como un generador de lenguajes.

  3. Quimera de ADN polimerasa del fago ϕ29

    OpenAIRE

    2010-01-01

    Quimera de ADN polimerasa del fago 29. La presente invención se encuadra dentro del campo de la biotecnología. Específicamente, se refiere a una quimera de ADN polimerasa que comprende una región aminoterminal (N-terminal), que codifica para una ADN polimerasa del tipo 29, y una región carboxilo-terminal (C-terminal), que comprende, al menos, un dominio HhH, que se encuentran unidas mediante una secuencia aminoacídica conectora y a su uso para la replicación, amplifi...

  4. Risk-taking propensity of nurses: ADN and BSN.

    Science.gov (United States)

    Masters, M L; Masters, R J

    1989-11-01

    Nursing literature indicates that skills in decision making are inherently important to the modern nurse. The literature also reports a controversy between the associate degree nurse (ADN) and the bachelor degree nurse (BSN). This conflict relates to educational level and experience in terms of leadership and decision-making skills. All decisions involve risk. Therefore decisions in nursing should be influenced by the risk-taking propensity of the individual nurse. The literature shows a linkage between risk-taking propensity and both training and experience. The major purpose of the study was to attempt to correlate risk-taking propensity of nurses by level of education and experience. The study investigated whether differences exist between risk-taking propensity of recently graduated ADNs and BSNs without experience. In addition, the study attempted to determine any differences in risk-taking propensity of ADNs and BSNs with practical experience. The results were analyzed in terms of decision-making competency.

  5. Effets des electrons secondaires sur l'ADN

    Science.gov (United States)

    Boudaiffa, Badia

    Les interactions des electrons de basse energie (EBE) representent un element important en sciences des radiations, particulierement, les sequences se produisant immediatement apres l'interaction de la radiation ionisante avec le milieu biologique. Il est bien connu que lorsque ces radiations deposent leur energie dans la cellule, elles produisent un grand nombre d'electrons secondaires (4 x 104/MeV), qui sont crees le long de la trace avec des energies cinetiques initiales bien inferieures a 20 eV. Cependant, il n'y a jamais eu de mesures directes demontrant l'interaction de ces electrons de tres basse energie avec l'ADN, du principalement aux difficultes experimentales imposees par la complexite du milieu biologique. Dans notre laboratoire, les dernieres annees ont ete consacrees a l'etude des phenomenes fondamentaux induits par impact des EBE sur differentes molecules simples (e.g., N2, CO, O2, H2O, NO, C2H 4, C6H6, C2H12) et quelques molecules complexes dans leur phase solide. D'autres travaux effectues recemment sur des bases de l'ADN et des oligonucleotides ont montre que les EBE produisent des bris moleculaires sur les biomolecules. Ces travaux nous ont permis d'elaborer des techniques pour mettre en evidence et comprendre les interactions fondamentales des EBE avec des molecules d'interet biologique, afin d'atteindre notre objectif majeur d'etudier l'effet direct de ces particules sur la molecule d'ADN. Les techniques de sciences des surfaces developpees et utilisees dans les etudes precitees peuvent etre etendues et combinees avec des methodes classiques de biologie pour etudier les dommages de l'ADN induits par l'impact des EBE. Nos experiences ont montre l'efficacite des electrons de 3--20 eV a induire des coupures simple et double brins dans l'ADN. Pour des energies inferieures a 15 eV, ces coupures sont induites par la localisation temporaire d'un electron sur une unite moleculaire de l'ADN, ce qui engendre la formation d'un ion negatif transitoire

  6. LVN to ADN: Innovative, Non-Traditional Learning Project.

    Science.gov (United States)

    Austin Community Coll., TX.

    This document contains the curriculum for the first nursing course in the Licensed Vocational Nurse Mobility Track Project. The project is designed to provide selected Licensed Vocational Nurses (LVNs) the opportunity to complete the nursing course requirements for an Associate of Applied Science Degree in Nursing (ADN) in three semesters of…

  7. The Oklahoma PN/ADN Articulation Project Report.

    Science.gov (United States)

    Oklahoma State Regents for Higher Education, Oklahoma City.

    In response to a critical nursing shortage in the state of Oklahoma, the Oklahoma Practical Nursing (PN)/Associate Degree Nursing (ADN) Articulation Project Coordinating Committee was formed in spring 1990 to develop a proposal for program articulation. A curriculum matrix was designed and adopted for use by five regional subcommittees which…

  8. How ADN students can learn prenatal (healthy) care.

    Science.gov (United States)

    Cranmer, J; Lajkowicz, C

    1989-01-01

    This article describes the problem-solving steps of how an unsupervised clinical experience can be successfully implemented at the ADN level so that large numbers of students can meet traditional clinical objectives in a nontraditional manner. Learning experiences covered are: patient teaching, interviewing, care planning - coordinated with theories of learning and program philosophy/purpose.

  9. A work-study course in an ADN program.

    Science.gov (United States)

    Farley, V M; Tyree, A

    1991-01-01

    This article describes a nursing student practicum in which students enrolled in the ADN program experience the real world of work over a five week period of time. The student's role and the RN mentor's role are described, as well as a report on the findings of this unique clinical elective in a basic nursing program.

  10. Significance of anti-deoxyribonuclease-B (ADN-B) determination in clinical practice.

    Science.gov (United States)

    Fujikawa, S; Kawakita, S; Kosakai, N; Oda, T; Ohkuni, M; Shiokawa, Y; Watanabe, N; Yamada, T

    1982-11-01

    The determination of anti-deoxyribonuclease-B (ADN-B) is very important for the diagnosis of antecedent streptococcal infection because almost all of group A streptococci have this antigen and a strong elevation of the antibody is observed in patients with acute rheumatic fever, acute glomerulonephritis and other streptococcal infections. Moreover, ADN-B titers are elevated in cases with streptococcal skin infection, whereas anti-streptolysin O (ASO) titers are rarely elevated in these cases. So the determination of ADN-B is recommended as the second streptococcal antibody test to ASO detection (manual of WHO). In our study, the upper limits of ADN-B in normal subjects were 1:60 in preschool age, 1:480 in school age and 1:340 in adult age groups. In acute rheumatic fever, ADN-B titers were elevated in 87% of the cases and also 87% of the patients with acute glomerulonephritis had high ADN-B titers. In inactive rheumatic fever, 72% of the patients had high ADN-B titers, while, ASO titers were elevated in only 22% of the cases. In streptococcal carrier states, 39% of the children had high ADN-B titers but ADN-B was positive only 8% in non-carrier children.

  11. Prueba del ADN y derecho a la intimidad: algunos conflictos concretos

    Directory of Open Access Journals (Sweden)

    Daniel Pérez González

    2014-12-01

    Full Text Available La prueba del ADN constituye una importante herramienta en campos muy diversos, como el de la investigación criminal o la prueba de paternidad, debido a que permite la identificación de los individuos a través de sus respectivos perfiles de ADN. Sin embargo, la aplicación de las técnicas de la prueba del ADN afecta a los derechos fundamentales. El almacenamiento y uso de la información recopilada en las bases de datos de ADN o las intervenciones corporales pueden constituir, bajo determinadas circunstancias, una violación o limitación de los derechos fundamentales. Este artículo pretende estudiar determinados conflictos relativos a la prueba del ADN y el derecho a la intimidad.

  12. ADN: huellas genéticas en el proceso penal

    OpenAIRE

    Ramallo Machín, Andrea Cristina

    2016-01-01

    [Resumen] El trabajo de investigación hace referencia a la aplicación de la identificación por el ADN de presuntos delincuentes en una investigación criminal como objeto de una prueba pericial en un proceso penal. Destacando las díferencias sustanciales que existen entre la aplicación de un método científico en circunstancias controladas en un laboratorio y su utilización en escenarios muy distintos, como puede ser el lugar donde se ha cometido un delito. Reconociendo su gra...

  13. Simulador de electroforesis de ADN en un gel virtual

    OpenAIRE

    Cross, Ismael; Rebordinos, Laureana

    2012-01-01

    El archivo consiste en una hoja de cálculo programada donde poder introducir los datos de diferentes muestras de ADN en pares de bases y observar y analizar cómo se verían en un gel de electroforesis e interpretar el patrón de bandas obtenido. El objetivo es poder realizar “electroforesis virtuales” en una práctica de bioinformática sin tener que realizar una práctica de laboratorio “ad hoc” cuando la electroforesis sea sólo una parte de dicha práctica.

  14. Energetic Characteristics and Comprehensive Properties of Propellants Containing ADN%含 ADN 推进剂的能量特性及综合性能

    Institute of Scientific and Technical Information of China (English)

    张伟; 谢五喜; 樊学忠; 刘芳莉; 庞维强; 刘小刚; 姬月萍

    2015-01-01

    In order to study the energy,safety,storage and combustion performances of propellants containing ammonium dinitramide (ADN),the energy characteristic parameters were calculated based on the minmum free energy mechanism.The heat of detonation and burning rate were tested by closed bomb test and target line method, and the moisture absorption and sensitivity were studied.The results show that the standard theory specific impulses of propellants containing ADN/Al/HMX,ADN/Al/CL-20,ADN/AlH3/HMX and ADN/AlH3/CL-20 are in the range of 2 675 -2 685,2 677 -2 686,2 801 -2 810 and 2 803 -2 812 N ·s ·kg-1 ,respectively.ADN propellant with normal curing and compact structure can be prepared using the inert polyether binder system plasti-cized by nitrate ester.With increasing the content of ADN in the propellant formulation,the heat of detonation, moisture absorption,burning rate and pressure exponent of the propellant increase,the friction and impact sensitivities of the propellant increase and the density decreases slightly.%为研究含二硝酰胺铵(ADN)推进剂的能量、安全、贮存及燃烧性能,根据最小自由能原理计算了含 ADN 推进剂的能量特性参数,采用密闭爆发器及靶线法测试其爆热及燃速,并对其吸湿性及感度进行了研究。结果表明,含 ADN/Al/HMX、ADN/Al/CL-20、ADN/AlH3/HMX 和 ADN/AlH3/CL-20推进剂的标准理论比冲分别为2675~2685、2677~2686、2801~2810和2803~2812 N·s·kg-1,采用硝酸酯增塑的惰性聚醚黏合剂体系可制备出固化正常、结构致密的含 ADN 推进剂。随着推进剂配方中 ADN 含量的增加,推进剂的爆热、吸湿性、燃速和压强指数增大,摩擦感度和撞击撞击提高,密度略有降低。

  15. The leadership/management role: a differentiating factor for ADN/BSN programs?

    Science.gov (United States)

    Schank, M J; Stollenwerk, R

    1988-06-01

    The resurgence of the ADN/BSN issue relative to educational preparation and roles and the concomitant development of positions taken by the professional associations and National Commission on Nursing pointed to the need for study of current ADN/BSN curricula and utilization of graduates. Based upon a brief historical literature review and ADN/BSN competency statements, the leadership/management role was selected for study as a possible differentiating factor between these programs. Findings from a statewide study of ADN and BSN programs and a representative sample of nurse executives indicated that an education and service dilemma exists relative to this role. The majority of ADN program directors noted that a leadership/management component should be part of ADN curricula, whereas BSN program directors did not concur. Nurse executives utilized ADN graduates in leadership/management positions and stated that length of experience was an influencing factor in selection. Numerous other findings showed a discrepancy in beliefs and practices among educators and nurse executives. This survey demonstrates the need for curriculum changes and role clarification. The leadership/management role may be a viable area for consideration.

  16. Said another way. Is the ADN graduate prepared to practice in community settings?

    Science.gov (United States)

    Percoco, T A

    1998-01-01

    The downsizing and closing of acute care facilities and the movement to community-based healthcare services are decreasing the need for RNs in acute care facilities. In the past, the associate-degree nurse (ADN) has filled the majority of positions in acute care. With the trend to provide health services in community setting, will the ADN be prepared for positions in community facilities? ADN educators must reevaluate how they are educating students for practice. The author reviews the 1995 recommendations from the Pew Health Professions Commission and relevant current directives from the National League for Nursing.

  17. LPN to ADN/BSN: accelerated curriculum for LPN's.

    Science.gov (United States)

    White-Parson, W F

    1993-01-01

    This article describes a special project involving the design, implementation and evaluation of a flexible education track for licensed practical nurses (LPN) within an established nursing program containing an associate degree in nursing (ADN) track and a baccalaureate degree in nursing (BSN) track. The LPN track facilitated the achievement of the associate degree or baccalaureate degree through curricular options which decreased the length of time and/or credit load for the LPN. A mechanism also was designed and implemented whereby credit for previous nursing knowledge and skills was awarded. Years 1 through 3 of the project focused on the advanced placement of LPNs into the associate degree program, and years 4 and 5 are an expansion to include the advanced placement of LPNs into the baccalaureate degree program.

  18. Development Testing of 1-Newton ADN-Based Rocket Engines

    Science.gov (United States)

    Anflo, K.; Gronland, T.-A.; Bergman, G.; Nedar, R.; Thormählen, P.

    2004-10-01

    With the objective to reduce operational hazards and improve specific and density impulse as compared with hydrazine, the Research and Development (R&D) of a new monopropellant for space applications based on AmmoniumDiNitramide (ADN), was first proposed in 1997. This pioneering work has been described in previous papers1,2,3,4 . From the discussion above, it is clear that cost savings as well as risk reduction are the main drivers to develop a new generation of reduced hazard propellants. However, this alone is not enough to convince a spacecraft builder to choose a new technology. Cost, risk and schedule reduction are good incentives, but a spacecraft supplier will ask for evidence that this new propulsion system meets a number of requirements within the following areas: This paper describes the ongoing effort to develop a storable liquid monopropellant blend, based on AND, and its specific rocket engines. After building and testing more than 20 experimental rocket engines, the first Engineering Model (EM-1) has now accumulated more than 1 hour of firing-time. The results from test firings have validated the design. Specific impulse, combustion stability, blow-down capability and short pulse capability are amongst the requirements that have been demonstrated. The LMP-103x propellant candidate has been stored for more than 1 year and initial material compatibility screening and testing has started. 1. Performance &life 2. Impact on spacecraft design &operation 3. Flight heritage Hereafter, the essential requirements for some of these areas are outlined. These issues are discussed in detail in a previous paper1 . The use of "Commercial Of The Shelf" (COTS) propulsion system components as much as possible is essential to minimize the overall cost, risk and schedule. This leads to the conclusion that the Technology Readiness Level (TRL) 5 has been reached for the thruster and propellant. Furthermore, that the concept of ADN-based propulsion is feasible.

  19. Differences in patient rating of care provided by BSN and ADN students.

    Science.gov (United States)

    Davis-Martin, S; Skalak, C

    1992-01-01

    Nursing has discussed widely and with fervor the level of education required to provide quality nursing care for clients. No clear consensus has developed but studies tend to show that especially in the hospital setting, baccalaureate (BSN) and associate degree (ADN) nurses initially practice at a similar level. No studies identified compared patient ratings of BSN and ADN nurses. In this study ratings of care provided by BSN and ADN students were compared. Patients, students and instructors rated the student sample using Watson's Patient Satisfaction Rating Scale. Using Pearson correlations and t-tests, the overall satisfaction with care was assessed as "very positive" by patients, faculty and students alike. A significant correlation was found between faculty and patient ratings. Results support earlier findings and demonstrate similarities rather than differences in care provided by the two levels of students. Implications for nursing education and practice are discussed.

  20. Adaptive Competency Acquisition: Why LPN-to-ADN Career Mobility Education Programs Work.

    Science.gov (United States)

    Coyle-Rogers, Patricia G.

    Adaptive competencies are the skills required to effectively complete a particular task and are the congruencies (balance) between personal skills and task demands. The differences between the adaptive competency acquisition of students in licensed practical nurse (LPN) programs and associate degree nurse (ADN) programs were examined in a…

  1. Feasibility of an LPN to ADN Articulation Program at LSUA. Vocational Education Research.

    Science.gov (United States)

    Louisiana State Univ., Alexandria.

    A feasibility study examined the need for and likelihood of success for a Licensed Practical Nurse (LPN) to Associate Degree in Nursing (ADN) articulation program for Louisiana State University (LSU) at Alexandria. Following a literature search on the theoretical establishment and implementation of such a program, three schools with successful…

  2. Aportaci??n a la m??tica historia de Ad??n y Eva (II)

    OpenAIRE

    1982-01-01

    Es continuaci??n de: Castillo Castillo, C. "Aportaci??n a la m??tica historia de Ad??n y Eva (I)". Miscel??nea de estudios ??rabes y hebraicos. Secci??n ??rabe-Islam. Vol. 29 (1980). ISSN 0544-408X, p. 35-52. [http://hdl.handle.net/10481/33730].

  3. Mechanism and kinetics for ammonium dinitramide (ADN) sublimation: a first-principles study.

    Science.gov (United States)

    Zhu, R S; Chen, Hui-Lung; Lin, M C

    2012-11-08

    The mechanism for sublimation of NH(4)N(NO(2))(2) (ADN) has been investigated quantum-mechanically with generalized gradient approximation plane-wave density functional theory calculations; the solid surface is represented by a slab model and the periodic boundary conditions are applied. The calculated lattice constants for the bulk ADN, which were found to consist of NH(4)(+)[ON(O)NNO(2)](-) units, instead of NH(4)(+)[N(NO(2))(2)](-), agree quite well with experimental values. Results show that three steps are involved in the sublimation/decomposition of ADN. The first step is the relaxation of the surface layer with 1.6 kcal/mol energy per NH(4)ON(O)NNO(2) unit; the second step is the sublimation of the surface layer to form a molecular [NH(3)]-[HON(O)NNO(2)] complex with a 29.4 kcal/mol sublimation energy, consistent with the experimental observation of Korobeinichev et al. (10) The last step is the dissociation of the [H(3)N]-[HON(O)NNO(2)] complex to give NH(3) and HON(O)NNO(2) with the dissociation energy of 13.9 kcal/mol. Direct formation of NO(2) (g) from solid ADN costs a much higher energy, 58.3 kcal/mol. Our calculated total sublimation enthalpy for ADN(s) → NH(3)(g) + HON(O)NNO(2)) (g), 44.9 kcal/mol via three steps, is in good agreement with the value, 42.1 kcal/mol predicted for the one-step sublimation process in this work and the value 44.0 kcal/mol computed by Politzer et al. (11) using experimental thermochemical data. The sublimation rate constant for the rate-controlling step 2 can be represented as k(sub) = 2.18 × 10(12) exp (-30.5 kcal/mol/RT) s(-1), which agrees well with available experimental data within the temperature range studied. The high pressure limit decomposition rate constant for the molecular complex H(3)N···HON(O)NNO(2) can be expressed by k(dec) = 3.18 × 10(13) exp (-15.09 kcal/mol/RT) s(-1). In addition, water molecules were found to increase the sublimation enthalpy of ADN, contrary to that found in the ammonium

  4. AISLAMIENTO DE ADN GENÓMICO DE Myrciaria dubia (HBK “CAMU CAMU” APROPIADO PARA ANÁLISIS MOLECULARES

    Directory of Open Access Journals (Sweden)

    Juan Carlos Castro Gómez

    2012-06-01

    Full Text Available Myrciaria dubia “camu-camu”, una especie nativa de la Amazonía que produce frutos con alto contenido de vitamina C y otras sustancias importantes. Sin embargo, los estudios moleculares de esta planta son escasos, por falta de un protocolo reproducible para purificar sus ácidos nucléicos. El objetivo de este trabajo fue establecer un protocolo para aislar el ADN genómico a partir de hojas de M. dubia, apropiado para análisis moleculares. El ADN se purificó con un protocolo modificado, la calidad y cantidad se estimó por espectrofotometría y electroforesis en gel de agarosa. Adicionalmente, la calidad se evaluó mediante RAPD. El ratio de calidad (A260/A280 promedio del ADN fue 1.9±0.1 y el espectro de absorción UV/Vis presentó un único pico de máxima absorbancia a 260nm. Mediante electroforesis el ADN fue íntegro y sin ARN. También, la síntesis de amplicones RAPD nos sugiere ausencia de inhibidores para polimerasas. La concentración promedio del ADN fue 99±33 ng/ml y el rendimiento promedio fue 237±80 mg ADN/g hoja. En conclusión, se ha establecido un protocolo de aislamiento de ADN genómico a partir de hojas de Myrciaria dubia “camu camu”, caracterizado por permitirnos obtener ADN de alta calidad y cantidad suficiente para análisis moleculares como el RAPD.

  5. Protection des ions organiques contre les dommages induits a l'ADN par les electrons de basse energie

    Science.gov (United States)

    Dumont, Ariane

    Il a ete demontre que les electrons de basse energie (EBE) peuvent induire des cassures simple brin (CSB) a l'ADN, via la formation d'anions transitoires qui decroissent par attachement dissociatif, ou dans d'autres etats electroniques dissociatifs menant a la fragmentation. Afin d'effectuer une etude complete des effets des electrons de basse energie sur la matiere biologique, il est necessaire de comprendre leur mecanismes d'interaction non seulement avec l'ADN, mais avec les constituants de son environnement. Les histones sont une composante importante de l'environnement moleculaire de l'ADN. Leur charge positive leur permet de s'associer aux groupements phosphate anionique de l'ADN. Le role principal de ces proteines basiques consiste a organiser l'ADN et l'empaqueter afin de former la chromatine. Les cations sont une autre composante importante de la cellule; ils jouent un role dans la stabilisation de la conformation B de l'ADN in vitro par leurs interactions avec les petits et grands sillons de l'ADN, ainsi qu'avec le groupement phosphate charge negativement. Avec les histones, ils participent egalement a la compaction de l'ADN pour former la chromatine. Cette etude a pour but de comprendre comment la presence d'ions organiques (sous forme de Tris et d'EDTA) a proximite de l'ADN modifie le rendement de cassures simple brin induit par les electrons de basse energie. Le Tris et l'EDTA ont-ete choisis comme objet d'etude, puisqu'en solution, ils forment le tampon standard pour solubiliser l'ADN dans les experiences in vitro (10mM Tris, 1mM EDTA). De plus, la molecule Tris possede un groupement amine alors que l'EDTA possede 4 groupements carboxyliques. Ensembles, ils peuvent se comporter comme un modele simple pour les acides amines. Le ratio molaire de 10 :1 de Tris par rapport a l'EDTA a pour but d'imiter le comportement des histones qui sont riches en arginine et lysine, acides amines possedant un groupement amine charge positivement additionnel. Des films d'ADN

  6. 安捷伦推出光学鼠标传感器ADNS-3060

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    安捷伦(Agilent)日前宣布推出一款基于LED的光学鼠标传感器ADNS-3060,它进一步改善了跟踪控制功能。与其他光学鼠标传感器相比,新的传感器在性能上更有所增强,可以在包括木质桌面和网纹图像等各种不同的表面上工作。ADNS-3060的表面跟踪精确度增强了20倍。

  7. Extracción del ADN de Fusarium Oxysporum f.sp. Dianthi

    OpenAIRE

    2010-01-01

    Se estudia la utílización de dos métodos para la extracción del ADN del Fusarium o.xy.sporum f.sp. Dianthi. En los dos métodos la pared del hongo se rompió con nitrógeno líquido, uno de ellos empleó como solución extractora bromuro de cetiltrimetil amonio (BCTA) y el otro una solución de sacarosa con altas concentraciones de protcasa y EDTA. Para la desproteinización ambos métodos utilizaron soluciones de fcnol-clorofonno y enzimas proteolítícas. El ADN obtenido se digirió con enzimas d...

  8. Enlaces de hidrógeno cooperativos en la interacción carbohidrato-ADN

    OpenAIRE

    2014-01-01

    Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Química Orgánica. Fecha de lectura: 22-05-2014 El título que aparece en la obra impresa es: "Enlaces de hidrógeno cooperativos en medio acuoso: glico-oligoamidas ligandos del surco menor del ADN"

  9. The level and focus of geriatric nursing content in ADN and BSN programs.

    Science.gov (United States)

    Fullerton, J T; Lantz, J; Quayhagen, M P

    1992-11-01

    The didactic and clinical focus of geriatric curriculum content within both associate (ADN) and baccalaureate (BSN) schools of nursing in California was reviewed. Geriatric nursing content experts confirmed the detail of a geriatric nursing curriculum, then determined which of the content items were basic to both educational levels, and which might be appropriately deferred to programs of baccalaureate preparation. Nurse educators may use the results of this study to guide curriculum development in both depth and breadth of content.

  10. Extracción del ADN de Fusarium Oxysporum f.sp. Dianthi

    Directory of Open Access Journals (Sweden)

    Sixta T. Martínez

    2010-07-01

    Full Text Available Se estudia la utílización de dos métodos para la extracción del ADN del Fusarium o.xy.sporum f.sp. Dianthi. En los dos métodos la pared del hongo se rompió con nitrógeno líquido, uno de ellos empleó como solución extractora bromuro de cetiltrimetil amonio (BCTA y el otro una solución de sacarosa con altas concentraciones de protcasa y EDTA. Para la desproteinización ambos métodos utilizaron soluciones de fcnol-clorofonno y enzimas proteolítícas. El ADN obtenido se digirió con enzimas de restricción EcoRI y Hindlll. Se corroboró que cl ADN estaba libre de los contaminantes más frecuentes en hongos, como proteínas y carbohidratos por medio de ultracentrifugación en cloruro de cesio (CsCI. La extracción con BCTA presentó los mejores rendimientos.

  11. ADN – The new oxidizer around the corner for an environmentally friendly smokeless propellant

    Directory of Open Access Journals (Sweden)

    Márcio Y. Nagamachi

    2009-06-01

    Full Text Available The search for a smokeless propellant has encouraged scientists and engineers to look for a chlorine-free oxidizer as a substitute for AP (ammonium perchlorate. Endeavors seemed to come to an end when ADN (ammonium dinitramide appeared in the West in the early 1990s. Although some drawbacks soon became apparent by that time, the foremost obstacle for its use in rocket-motors came from the patent originally applied for in the United States in 1990. Furthermore, environmental concerns have also increased during these two decades. Ammonium perchlorate is believed to cause thyroid cancer by contaminating soil and water. In addition, AP produces hydrogen chloride during burning which can cause acid rain and ozone layer depletion. Unlike AP, ADN stands for both smokeless and green propellant. Since then, much progress has been made in its development in synthesis, re-shaping, microencapsulation and solid propellant. The high solubility of ADN in water has also allowed its application as liquid monopropellant. Tests have revealed Isp (specific impulse superior to that normally observed with hydrazine, one of the most harmful and hazardous liquid propellants. With constraints of use, along with the patent near to expiry, scientists and engineers are rushing to complete developments and patents until then.

  12. Estructura cristalina de ADN y su utilización para la identificación de fármacos

    OpenAIRE

    Coll, Miquel; Aymamí, Joan

    2006-01-01

    Estructura cristalina de ADN y su utilización para la identificación de fármacos. La estructura cristalina que comprende un entrecruzamiento de tres cadenas de ADN está caracterizada porque: pertenece al grupo espacial P4(1)32 y tiene unas dimensiones de celda a=b=c=70,98 ± 0,7°A; todos los nucleótidos que constituyen dichas cadenas de ADN se encuentran emparejados; y dicho entrecruzamiento de tres cadenas de ADN alberga, en una cavidad hidrofóbica, una molécula. La...

  13. GAP/ADN/nano-Al膏体推进剂的能量特性与激光点火特性%Energy characteristics and laser ignition characteristics of GAP/ADN/nano-Al pasty propellants

    Institute of Scientific and Technical Information of China (English)

    裴庆; 郝海霞; 赵凤起; 徐司雨; 姚二岗; 汪营磊

    2015-01-01

    采用最小自由能原理方法计算了GAP(聚叠氮缩水甘油醚)/ADN(二硝酰胺铵)/nano-Al推进剂的能量特性,制备了一系列ADN质量分数为8%~38%的GAP/ADN/nano-Al膏体推进剂,采用CO2激光点火的方法研究了4种配方在不同激光功率密度作用下的激光点火特性.结果表明:GAP/ADN/nano-Al膏体推进剂的标准理论比冲(Isp)、特征速度(C*)、燃烧温度(Tc)均随ADN含量增加而依次增大,爆热(Qv)则主要随铝粉含量的增加而增大;GAP/ADN/nano-Al膏体推进剂的点火延迟时间和点火能量总体上随着激光功率密度增加呈现减小的趋势;配方中ADN含量较高时,GAP/ADN/nano-Al膏体推进剂具有较好的激光点火特性.

  14. A novel implantable vagus nerve stimulation system (ADNS-300) for combined stimulation and recording of the vagus nerve: Pilot trial at Ghent University Hospital

    NARCIS (Netherlands)

    El Tahry, R.; Raedt, R.; Mollet, L.; de Herdt, V.; Wyckuys, T.; Van Dycke, A.; Meurs, A.; Dewaele, F.; van Roost, D.; Doguet, P.; Delbeke, J.; Wadman, W.; Vonck, K.; Boon, P.

    2010-01-01

    Purpose: Vagus nerve stimulation (VNS) is an established treatment for refractory epilepsy. The ADNS-300 is a new system for VNS that includes a rechargeable stimulus generator and an electrode for combined stimulation and recording. In this feasibility study, three patients were implanted with ADNS

  15. 基于光电传感器ADNS-2030无线鼠标的设计与实现%Design and implementation of wireless mouse based on ADNS-2030

    Institute of Scientific and Technical Information of China (English)

    赵升; 陈庆来; 解瑞杰

    2006-01-01

    研究基于光电传感器ADNS-2030的无线鼠标设计,讨论了ADNS-2030无线鼠标的软件、硬件设计和系统组成,通过实际制作表明:无线鼠标的设计方案是合理可行的,该产品具有性价比高,功耗低,高分辨率,有效距离远等优点,具有一定的应用前景和推广价值.

  16. Comparison of role conception and role deprivation in LPN-transition students and traditional ADN students in a specially designed associate program.

    Science.gov (United States)

    Lengacher, C A; Keller, R

    1992-02-01

    This study compared differences in role orientation and role deprivation between 41 LPN-transition students and 123 basic traditional associate degree nursing (ADN) students upon entry and exit to a specially designed ADN program. The Corwin Role Conception Scale was used to assess role conception and deprivation. Statistically significant differences were noted upon entry between LPN-transition students and traditional ADN students in professional and service role conceptions and in role deprivation, upon exit in professional role conception and role deprivation between LPN-transition and traditional ADN students, and in professional role conception within the LPN-transition students. No differences were found within traditional ADN students from entry to exit of the program.

  17. Avago发布USB光学鼠标传感器SOC ADNS-2700

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Avago Technologies近日宣布推出一款光学传感器片上系统(SoC),为鼠标和其他人机接口设备提供市场上最小的引脚数。这款高度集成的紧凑型ADNS-2700LED传感器无需使用外部微控制器,使用更少的无源元件,可提供简易设计并降低整体系统成本。

  18. Extracción de adn plasmidico de cepas de rhizobium leguminosarum

    Directory of Open Access Journals (Sweden)

    M. Y, Cortés L.

    2010-07-01

    Full Text Available S.e realizó una comparación simultánea de cuatro métodos para la extracción de ADN plasmídico. De los resultados experimentales se concluyó que únicamente con la técnica de Kronstad, en las condiciones ensayadas y con cultivos de cepas de Rhizobium leguminosarum bv Viceae en fase de crecimiento logarítmica temprana, se visualizaron 3 plásmidos para la cepa SEMIA 335 y un plásmido para la cepa B.

  19. Extracción de adn plasmidico de cepas de rhizobium leguminosarum

    OpenAIRE

    2010-01-01

    S.e realizó una comparación simultánea de cuatro métodos para la extracción de ADN plasmídico. De los resultados experimentales se concluyó que únicamente con la técnica de Kronstad, en las condiciones ensayadas y con cultivos de cepas de Rhizobium leguminosarum bv Viceae en fase de crecimiento logarítmica temprana, se visualizaron 3 plásmidos para la cepa SEMIA 335 y un plásmido para la cepa B.

  20. Adulteriode la mujer, presunción de paternidad y la prueba oportuna del ADN

    OpenAIRE

    Boada Freire, Denys Paúl

    2015-01-01

    En la actualidad, nuestro entorno y el medio en el que nos desenvolvemos conocida también como una sociedad civilizada, ha sufrido cambio debido al alto grado de disolución de hogares a causa de la infidelidad conocido jurídicamente como adulterio. El presente trabajo de investigación, trata sobre el adulterio específicamente de la mujer, la presunta prueba de paternidad mediante la utilización de la prueba del ADN, se trata de un tema muy relevante en nuestra sociedad, ya que el adulterio...

  1. DETERMINACIÓN DEL SEXO POR ADN EN CINCO ESPECIES DE GUACAMAYOS

    OpenAIRE

    Liza R., Jacqueline; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Maturrano H., Lenin; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Rosadio A., Raúl; Laboratorio de Microbiología y Parasitología Veterinaria, Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima

    2012-01-01

    La determinación del sexo en aves de especies silvestres es de vital importancia para tomar medidas de conservación. En especies donde no existe dimorfismo sexual, es necesario contar con una prueba de sexaje alternativo a los métodos quirúrgicos. En este sentido, con la finalidad de estandarizar una prueba para la determinación del sexo en guacamayos, se desarrolló y evaluó una prueba molecular que consistió en el análisis de ADN mediante la Reacción en Cadena de la Polimerasa (PCR). Para el...

  2. LOS METALES COMO INHIBIDORES DEL SISTEMA DE REPARACIÓN DEL ADN

    OpenAIRE

    Pablo Hernández-Franco; Mahara Valverde; Emilio Rojas

    2009-01-01

    Los humanos han estado en contacto con los metales desde su aparición en el planeta. De hecho no se puede pensar en la evolución humana sin pensar en el gran papel que han jugado los metales en el desarrollo humano. Algunos metales son esenciales para llevar a cabo procesos celulares, sin embargo, otros son capaces de promover una gran variedad de efectos deletéreos para la salud, incluyendo el cáncer. Las evidencias de que la inhibición de los mecanismos de reparación del ADN, es un importan...

  3. 球形二硝酰胺铵研究%Study on prilled ammonium dinitramide(ADN)

    Institute of Scientific and Technical Information of China (English)

    马跃; 张海林

    2002-01-01

    介绍了二硝酰胺铵(ADN)的液相凝聚成球方法,并对球形ADN的吸湿性、感度(撞击、摩擦)、粒度分布以及颗粒表面进行了测试和分析.结果表明,球形ADN吸湿性、感度优于非球形ADN.用这种方法制得的球形ADN粒径为5~800μm.

  4. Extracción de ADN y una prueba inical de primers en Pinus pseudostrobus Lindl. Para marcadores AFLP

    OpenAIRE

    2010-01-01

    Se desarrollo un método de extracción de ADN simple, rápido y de alto rendimiento para Pinus pseudostrobus Lindl. del área semillera establecida en Jerahuaro, Michoacán para la producción de semilla de calidad. Se implementaron herramientas moleculares (AFLP) para identificar su relación genética inter-especie. Es muy importante contar con el protocolo de extracción y purificación de ADN estandarizado para la especie de interés, debido a que este proceso constituye una etapa clave en todos lo...

  5. Simultaneous heterotrophic nitrification and aerobic denitrification by the marine origin bacterium Pseudomonas sp. ADN-42.

    Science.gov (United States)

    Jin, Ruofei; Liu, Tianqi; Liu, Guangfei; Zhou, Jiti; Huang, Jianyu; Wang, Aijie

    2015-02-01

    Recent research has highlighted the existence of some bacteria that are capable of performing heterotrophic nitrification and have a phenomenal ability to denitrify their nitrification products under aerobic conditions. A high-salinity-tolerant strain ADN-42 was isolated from Hymeniacidon perleve and found to display high heterotrophic ammonium removal capability. This strain was identified as Pseudomonas sp. via 16S rRNA gene sequence analysis. Gene cloning and sequencing analysis indicated that the bacterial genome contains N2O reductase function (nosZ) gene. NH3-N removal rate of ADN-42 was very high. And the highest removal rate was 6.52 mg/L · h in the presence of 40 g/L NaCl. Under the condition of pure oxygen (DO >8 mg/L), NH3-N removal efficiency was 56.9 %. Moreover, 38.4 % of oxygen remained in the upper gas space during 72 h without greenhouse gas N2O production. Keeping continuous and low level of dissolved oxygen (DO <3 mg/L) was helpful for better denitrification performance. All these results indicated that the strain has heterotrophic nitrification and aerobic denitrification abilities, which guarantee future application in wastewater treatment.

  6. Descifrando los mensajes del pasado : análisis del ADN antiguo

    Directory of Open Access Journals (Sweden)

    Concepción de la Rúa

    2005-01-01

    Full Text Available El análisis de ADN (ADNa nos permite estudiar directamente la composición genética de las poblaciones del pasado. Sin embargo, pronto se hicieron patentes las limitaciones metodológicas derivadas del mal estado de preservación del ADNa, así como del enorme riesgo de contaminación con ADN humano momderno. Ello, condujo a la elaboración de una serie de Criterios de Autentificación que deben cumplir todos los trabajos del campo de ADNa para su publicación. Actualmente, los trabajos publicados no son numerosos, ni espectaculares, pero los resultados son reproducibles y su autenticidad esta demostrada. Nuestro equipo viene trabajando desde hace más de 10 años en este campo. Hemos analizado 5 yacimientos prehistóricos (SJaPL, Longar, Pico Ramos, Urratxa y Aizpea y uno histórico (Aldaieta del País Vasco. Los resultados obtenidos hasta el momento, nos están permitiendo indagar en los procesos evolutivos sufridos por la población vasca, constrastar las hipótesis que han sido planteadas con otras evidencias, y concretamente en el yacimiento de Aldaieta, indagar en cuestiones biosociales, como el parentesco, y en el origen de este grupo humano.

  7. Modélisation des effets de la photoabsorption K sur l'ADN

    Science.gov (United States)

    Vrigneaud, J. M.; Terrissol, M.

    1999-01-01

    Monte Carlo codes were used to simulate the transport of photons and their secondary electrons produced on a linear and hydrated DNA model. Between 150 and 550eV, the variation of photon cross section upon the K-ionisation threshold in C, N, O atoms of DNA is responsible for an increase of the biological effectiveness. We showed that, with the same number of absorbed photons, the distribution of breaks as a function of energy reaches a maximum around 450eV. Nous avons simulé le transport des photons et de leurs électrons secondaires mis en mouvement dans un modèle d'ADN linéaire hydraté, en utilisant la méthode de Monte Carlo. Entre 150 et 550 eV, la variation de section efficace photonique au- dessus du seuil d'ionisation K des atomes C, N, O de l'ADN est responsable d'un accroissement de l'efficacité biologique. Nous avons montré que, pour un même nombre de photons absorbés, la distribution des cassures en fonction de l'énergie passe par un maximum autour de 450 eV.

  8. Ensayos cristalográficos de complejos de ADN con proteínas HMG-box y fármacos

    OpenAIRE

    Gomez Jimenez, Fabiola Alejandra

    2016-01-01

    Las HMGB son proteínas nucleares que presentan el motivo “HMG-box”, con el que se unen al surco estrecho del ADN. Producen cambios estructurales en el mismo y están implicadas en diferentes enfermedades por lo que el estudio estructural de dichas proteínas unidas a ADN es de importancia en el desarrollo de estrategias terapéuticas. Por otra parte, los compuestos derivados de difenilo bisimidazolinio también se unen al surco estrecho del ADN, específicamente en zonas ricas en AT...

  9. Estudio de la fragmentación en el ADN espermático en pacientes sometidos a técnicas de reproducción asistida

    OpenAIRE

    Esbert Algam, Margarida

    2015-01-01

    La integridad del ADN del espermatozoide es de vital importancia, dado que puede comprometer la viabilidad del embrión. Para cuantificar la fragmentación en el ADN espermático se han desarrollado distintas técnicas, que han servido para relacionar dicha fragmentación con la esterilidad de origen masculino. Teniendo en cuenta que algunos de los individuos estériles van a someterse a técnicas de reproducción asistida, se ha valorado si la fragmentación en el ADN espermático i) disminuye tras pr...

  10. A unique strategy for pediatric community health nursing for ADN students.

    Science.gov (United States)

    Janvier, K A

    1999-01-01

    Students were overwhelmingly positive when given the opportunity to evaluate the pilot project and the model of pediatric community health nursing. According to the students, the strong points of the model were the orientation before the community experience, the presence of faculty of the community, the ability to contact faculty when needed, and the postclinical conference. The students' comments confirmed the faculty's belief that a clinical experience in community health nursing must place more emphasis on the specialty of community health nursing to be meaningful for students. To do the of job of educating tomorrow's nurses, ADN faculty should develop new strategies for teaching the pediatric clinical component of community health nursing. Clearly, hospitals are no longer the exclusive sites where students learn about patient and family needs and nursing care delivery. Community-based and community-focused experiences will continue to be required so that nursing students are prepared to practice in a dynamic and changing healthcare environment.

  11. Outcomes of ADN-BSN partnerships to increase baccalaureate prepared nurses.

    Science.gov (United States)

    Sizemore, Mary H; Robbins, Leslie K; Hoke, Mary M; Billings, Diane M

    2007-01-01

    The limited supply of BSN nurses hinders efforts to increase patient care quality and address health disparities. In largely rural and economically disadvantaged areas, associate degree prepared nurses provide the majority of nursing services. To address a statewide need, a BSN Program and 3 ADN Programs formed a partnership to take BSN education to rural and medically underserved areas. This article describes the program planning, implementation, and evaluation using an adapted assessment framework with partnership principles as its foundation. Interactive television, internet education components, local clinical experiences, and distant nursing faculty liaisons were used. The nursing course sequence was completed by 101 of 102 students. Hall's Professionalism Scale, the California Critical Thinking Disposition Inventory, and the California Critical Thinking Skills Test measured the increases found in professional socialization and critical thinking. Use of the adapted theoretical framework represented a strategic approach to developing a distance delivered nursing education program.

  12. Variantes de la concatenación en computación con ADN

    OpenAIRE

    Rodríguez-Patón Aradas, Alfonso

    1999-01-01

    Las principales aportaciones de esta Tesis Doctoral a la computación con ADN y en general, a las ciencias de la computación, se pueden clasificar en dos áreas básicas: una práctica o algorítmica y otra teórica o de modelización de operaciones sobre palabras y lenguajes. Más en detalle, las aportaciones prácticas y teóricas se pueden describir como sigue. Aportaciones prácticas. Se han diseñado bio-algoritmos evolutivos para el Problema del Camino de Hamilton dirigido y se han ideado nuevos es...

  13. The ADN project : an integrated seismic monitoring of the northern Ecuadorian subduction

    Science.gov (United States)

    Nocquet, Jean-Mathieu; Yepes, Hugo; Vallee, Martin; Mothes, Patricia; Regnier, Marc; Segovia, Monica; Font, Yvonne; Vaca, Sandro; Bethoux, Nicole; Ramos, Cristina

    2010-05-01

    The subduction of the Nazca plate beneath South America has caused one of the largest megathrust earthquake sequence during the XXth century with three M>7.7 earthquakes that followed the great 1906 (Mw = 8.8) event. Better understanding the processes leading to the occurrence of large subduction earthquakes requires to monitor the ground motion over a large range of frequencies. We present a new network (ADN) developed under a collaboration between the IRD-GeoAzur (Nice, France) and the IG-EPN (Quito, Ecuador). Each station of the ADN network includes a GPS recording at 5 Hz, an accelerometer and a broadband seismometer. CGPS data will quantify the secular deformation induced by elastic locking along the subduction interface, enabling a detailed modelling of the coupling distribution. CGPS will be used to monitor any transient deformation induced by Episodic Slip Event along the subduction, together with broadband seismometers that can detect any tremors or seismic signatures that may accompany them. In case of any significant earthquake, 5 Hz GPS and accelerometer will provide near field data for earthquake source detailed study. Finally, the broadband seismometers will be used for study of the microseismicity and structure of the subduction zone. The network includes 9 stations, operating since 2008 and covering the coastal area from latitude 1.5°S to the Colombian border. In this poster, we will present preliminary assessment of the data, first hypocenters location, magnitude and focal mechanism determination, as well as results about an episodic slip event detected in winter 2008.

  14. Experimental investigation of the catalytic decomposition and combustion characteristics of a non-toxic ammonium dinitramide (ADN)-based monopropellant thruster

    Science.gov (United States)

    Chen, Jun; Li, Guoxiu; Zhang, Tao; Wang, Meng; Yu, Yusong

    2016-12-01

    Low toxicity ammonium dinitramide (ADN)-based aerospace propulsion systems currently show promise with regard to applications such as controlling satellite attitude. In the present work, the decomposition and combustion processes of an ADN-based monopropellant thruster were systematically studied, using a thermally stable catalyst to promote the decomposition reaction. The performance of the ADN propulsion system was investigated using a ground test system under vacuum, and the physical properties of the ADN-based propellant were also examined. Using this system, the effects of the preheating temperature and feed pressure on the combustion characteristics and thruster performance during steady state operation were observed. The results indicate that the propellant and catalyst employed during this work, as well as the design and manufacture of the thruster, met performance requirements. Moreover, the 1 N ADN thruster generated a specific impulse of 223 s, demonstrating the efficacy of the new catalyst. The thruster operational parameters (specifically, the preheating temperature and feed pressure) were found to have a significant effect on the decomposition and combustion processes within the thruster, and the performance of the thruster was demonstrated to improve at higher feed pressures and elevated preheating temperatures. A lower temperature of 140 °C was determined to activate the catalytic decomposition and combustion processes more effectively compared with the results obtained using other conditions. The data obtained in this study should be beneficial to future systematic and in-depth investigations of the combustion mechanism and characteristics within an ADN thruster.

  15. Relation of Na+, K(+)-ATPase to delayed motor nerve conduction velocity: effect of aldose reductase inhibitor, ADN-138, on Na+, K(+)-ATPase activity.

    Science.gov (United States)

    Hirata, Y; Okada, K

    1990-06-01

    The role of sorbitol, myo-inositol, and Na+, K(+)-adenosine triphosphatase (ATPase) activity on motor nerve conduction velocity (MNCV) in streptozotocin (STZ)-diabetic rats was studied. Reduction of MNCV and Na+, K(+)-ATPase in caudal nerves appeared after 3 weeks of diabetes, and at this time treatment with aldose reductase inhibitor (ARI), ADN-138 and 1% myo-inositol supplement was begun. One percent myo-inositol supplement for 3 weeks resulted in a significant increase in myo-inositol levels in diabetic nerves, but left MNCV and sorbitol levels unchanged. In contrast, treatment with ADN-138 for 3 weeks reduced sorbitol levels in diabetic nerves and resulted in significant increases in MNCV and Na+, K(+)-ATPase in the nerves. Since ADN-138 did not restore myo-inositol levels, the increase in Na+, K(+)-ATPase levels by ADN-138 treatment was independent of myo-inositol levels. Also, nerve Na+ levels in ADN-138-treated rats were reduced and the ratio of K+ to Na+ was raised, while 1% myo-inositol supplement did not affect them. These results suggest that treatment with ADN-138 elevates MNCV through a series of processes: ARI----reduction of sorbitol level----increase in Na+, K(+)-ATPase activity----correction of K+, Na+ imbalance----increase in MNCV.

  16. Short of transformation: American ADN students' thoughts, feelings, and experiences of studying abroad in a low-income country.

    Science.gov (United States)

    Foronda, Cynthia L; Belknap, Ruth Ann

    2012-06-03

    ADN students are a large yet distinct subgroup of nursing students who require research and understanding. The purpose of this study was to describe the thoughts, feelings, and experiences of American associate degree nursing (ADN) students who participated in a short study abroad course in a low-income country. A qualitative, narrative method was used. Three categories emerged from the analysis. Participants revealed thoughts of "constant comparisons", feelings of an "emotional journey", and they experienced "learning". Participants did not demonstrate perspective transformation as defined by Mezirow as participants signified no intent for social action. Several potential blocks to perspective transformation were identified: egocentrism/emotional disconnect, perceived powerlessness/being overwhelmed, and a vacation mindset. The findings provide insight into the student experience of studying abroad. Transformative learning is not a guaranteed result. Nurse educators must consider strategies to foster transformation including discussing global systemic oppressors, international relations, coping, connecting, and social action.

  17. La regulación de los datos genéticos y las bases de datos de ADN

    OpenAIRE

    Guerrero Moreno, Álvaro Alfonso

    2011-01-01

    Hoy en día la utilización del ADN es un medio esencial para realizar investigación en el ámbito de la administración de justicia y es una prueba usada y aceptada universalmente tanto en la esfera de investigación biológica de la paternidad o maternidad (procesos civiles) y en materia penal; igualmente el uso del ADN es de gran utilidad para la investigación de cadáveres y personas desaparecidas. Se tratarán algunos de los problemas que se han generado en relación con los avances en materia ge...

  18. Revisión sobre la extracción de ADN a partir de huesos humanos

    Directory of Open Access Journals (Sweden)

    Gerardo Jiménez-Arce

    1999-09-01

    Full Text Available Los huesos antiguos son una buena fuente de ADN, útil en muchos casos para el análisis forense, especialmente cuando el tejido blando está severamente descompuesto o dañado. Existen diferentes métodos para la extracción de ADN a partir de restos óseos, con diferentes eficacias, y que plantean varios problemas técnicos. Se presenta una revisión bibliográfica sobre este tema y se incluyen algunas recomendaciones prácticas.Ancient bones are a good source of DNA, useful on many forensic cases, specially when the soft tissues are putrefied to a high extent. There is many different methods to extract DNA from bones with various degrees of efficiency on the DNA amount obtained. We present a bibliographic review and some practical considerations.

  19. PROTOCOLO PARA LA EXTRACCIÓN DE ADN METAGENÓMICO BACTERIANO DEL LANGOSTINO Macrobrachium carcinus L

    OpenAIRE

    J. Ulises González-de la Cruz; H. Delfin-González; Ma. C. de la Cruz-Leyva; R. A. Rojas-Herrera; M. Zamudio-Maya

    2011-01-01

    En este trabajo se adecuó un protocolo para la extracción de ADN metagenómico (ADNmg) bacteriano del sistema digestivo (intestino, estómago y hepatopáncreas) del langostino Macrobrachium carcinus L., tomando como referencia la metodología de extracción de ADN bacteriano de suelos y sedimentos (Rojas-Herrera et al., 2008). Esta metodología constaba de lisis enzimática, física, mecánica y química; después de una serie de ensayos se suprimió la lisis enzimática. Sin embargo, el éxito de la extra...

  20. RN-BSN Students' Perceptions of the Differences in Practice of the ADN- and BSN-Prepared RN.

    Science.gov (United States)

    Matthias, April D; Kim-Godwin, Yeoun Soo

    2016-01-01

    This study explored RN-BSN students' perceptions of practice differences between nurses prepared with an ADN and BSN. Five themes were identified in 20 students' discussion posts: "a nurse is a nurse" at the bedside, beyond the bedside, BSN wanted, digging deeper, and appraisal. Results illustrate the need for educators to assist nurses in translating the differentiated educational competencies to the practice role of the bedside RN.

  1. 艾默生推出两款ADN-C系列小巧型DIN导轨电源

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    日前,艾默生网络能源公司宣布推出两款属于ADN-C系列的小巧型DIN导轨安装三相电源产品。这两款10A和5A产品的推出令该系列电源的产品阵容显得更为鼎盛。

  2. Vacunas de ADN: inducción de la respuesta inmunitaria DNA Vaccines: Induction of the immune response

    Directory of Open Access Journals (Sweden)

    Javier Mota-Sánchez

    2009-01-01

    Full Text Available La efectividad de las vacunas y la inmunización en la prevención de las enfermedades infecciosas es uno de los grandes avances de la medicina. En la actualidad, el acceso a la tecnología de punta en el área de la genómica y la proteómica ha hecho posible acelerar el desarrollo de nuevos modelos de vacunas con características mejoradas en aspectos fundamentales, como la inmunogenicidad y la seguridad. A casi dos décadas del primer informe, en el cual se demostró que un gen puede expresarse mediante la inyección directa de ADN desnudo, las vacunas de ADN han probado ser eficientes para inducir una respuesta inmunitaria protectora contra parásitos, virus y bacterias en diversos modelos animales. Esta revisión tiene por objetivo presentar un panorama general de las vacunas de ADN y los mecanismos mediante los cuales la inmunización con antígenos insertados en vectores de ADN (plásmidos inducen una respuesta inmunitaria.The effectiveness of vaccines and immunization in the prevention of infectious diseases is one of the greatest successes in medicine. In recent years, with access to cutting edge genomic and proteomic technology, it is possible to accelerate the development of new and improved vaccines with better immunogenicity and safety characteristics. Since the first report almost two decades ago, where it was demonstrated that gene expression is possible by directed injection of naked DNA, DNA vaccines have been proven to induce protective immune responses against parasites, virus and bacterium in diverse animal disease models. This review aims to present an overview about DNA vaccines and the mechanisms by which immune responses are induced after immunization with plasmid DNA-encoded antigens.

  3. Baroreflexes of the rat. V. Tetanus-induced potentiation of ADN A-fiber responses at the NTS.

    Science.gov (United States)

    Tang, Xiaorui; Dworkin, Barry R

    2007-12-01

    In a long-term neuromuscular blocked (NMB) rat preparation, tetanic stimulation of the aortic depressor nerve (ADN) enhanced the A-fiber evoked responses (ERs) in the cardiovascular region, the nucleus of the solitary tract (dmNTS). The potentiation persisted for at least several hours and may be a mechanism for adaptive adjustment of the gain of the baroreflex, with functional implications for blood pressure regulation. Using a capacitance electrode, we selectively stimulated A-fibers and acquired a stable 10-h "A-fiber only" ER baseline at the dmNTS. Following baseline, an A+C-fiber activating tetanus was applied to the ADN. The tetanus consisted of 1,000 "high current" pulses (10 trains; 300 mus, 100 Hz, 1 s), with intertrain interval of 9 s. A 10-h A-fiber only posttetanic test phase repeated the stimulus pattern of the baseline. Fourteen tetanus experiments were done in 12 rats. Compared with the baseline before tetanus, the A-fiber ER magnitudes of posttetanus hours were larger [F(13, 247) = 3.407, P ADN A+C fiber-activating tetanus produced increases in the magnitude of the A-fiber ERs in the dmNTS that persisted for several hours. In an additional rat, application of an NMDA receptor antagonist, prior to the tetanus, blocked the potentiation effect. The stimulus protocols, magnitude and duration of the effect, and pharmacology resemble associative long-term potentiation (LTP).

  4. Identificación de restos óseos humanos mediante análisis de ADN

    Directory of Open Access Journals (Sweden)

    Bernal Morera-Brenes

    1998-12-01

    Full Text Available En los últimos años la tecnología del ADN se ha convertido en una poderosa herramienta para la identificación individual, incluso cuando sólamente se cuenta con restos óseos. Se ilustra un caso en el cual se realizó la prueba de identificación comparando el ADN de los supuestos padres contra el ADN extraído del hueso de un menor de sexo femenino, utilizando los marcadores: HLA-DQA1, LDLR, GYPA, HBGG, D7S8, Gc, y D1S80. Los perfiles genéticos obtenidos permitieron concluir una identificación positiva de los restos como pertenecientes a la niña desaparecida. Esta metodología queda disponible para futuros casos forenses que así lo ameriten.The DNA technology has become a powerful tool for human identification in the last few years even in cases where the bones are the only remains. We now report the successful identification of the skeletal remains of a murder victim by comparative typing on nuclear DNA in remains, and in the presumptive parents of the victim. The genetic markers used were: HLA-DQA1, LDLR, GYPA, HBGG, D7S8, Gc, and D1S80. This feasibility of bone DNA typing is available in forensic investigation in Costa Rica now.

  5. Comparación de dos protocolos de extracción de ADN de Trypanosoma cruzi cultivados en medio axénico

    Directory of Open Access Journals (Sweden)

    Mariela López

    Full Text Available Objetivos. Comparar dos protocolos de extracción de ADN de Trypanosoma cruzi para su uso en la amplificación de ADN de minicírculos de kinetoplasto (ADNk mediante la técnica de Reacción en Cadena de Polimerasa (PCR. Materiales y métodos. Se cultivaron epimastigotas de T. cruzi en condiciones exénicas obteniéndose masas entre 1,5 hasta 100 x 10(6 parásitos. A partir de estas se procedió a la extracción de ADN mediante dos protocolos: extracción con solventes orgánicos (fenol/cloroformo, y empleo de resina (Chelex®100, a partir de los diferentes sedimentos parasitarios. La concentración y pureza del ADN se determinó por espectrofotometría y la integridad se evaluó mediante electroforesis en geles de agarosa. Se realizó el análisis de varianza y comparaciones de medias mediante la prueba de Tukey, utilizando el software Statistix 8.0. Resultados. Se realizaron diez extracciones de ADN de cada una de las diferentes cantidades de parásitos sedimentados. En la extracción de ADN con la resina Chelex®100 se obtuvo mayor rendimiento, pero menor pureza e integridad respecto a la extracción con solventes orgánicos. Sin embargo, permitió la amplificación del producto de 330 pb de ADNk de T. cruzi. Conclusiones. Aun cuando la técnica de Chelex®100 proporcionó menor pureza e integridad del ADN, permitió la amplificación con éxito de ADNk por PCR, evitando el uso de técnicas laboriosas y solventes orgánicos tóxicos

  6. Comparación de dos protocolos de extracción de ADN de Trypanosoma cruzi cultivados en medio axénico

    Directory of Open Access Journals (Sweden)

    Mariela López

    2014-04-01

    Full Text Available Objetivos. Comparar dos protocolos de extracción de ADN de Trypanosoma cruzi para su uso en la amplificación de ADN de minicírculos de kinetoplasto (ADNk mediante la técnica de Reacción en Cadena de Polimerasa (PCR. Materiales y métodos. Se cultivaron epimastigotas de T. cruzi en condiciones exénicas obteniéndose masas entre 1,5 hasta 100 x 10(6 parásitos. A partir de estas se procedió a la extracción de ADN mediante dos protocolos: extracción con solventes orgánicos (fenol/cloroformo, y empleo de resina (Chelex®100, a partir de los diferentes sedimentos parasitarios. La concentración y pureza del ADN se determinó por espectrofotometría y la integridad se evaluó mediante electroforesis en geles de agarosa. Se realizó el análisis de varianza y comparaciones de medias mediante la prueba de Tukey, utilizando el software Statistix 8.0. Resultados. Se realizaron diez extracciones de ADN de cada una de las diferentes cantidades de parásitos sedimentados. En la extracción de ADN con la resina Chelex®100 se obtuvo mayor rendimiento, pero menor pureza e integridad respecto a la extracción con solventes orgánicos. Sin embargo, permitió la amplificación del producto de 330 pb de ADNk de T. cruzi. Conclusiones. Aun cuando la técnica de Chelex®100 proporcionó menor pureza e integridad del ADN, permitió la amplificación con éxito de ADNk por PCR, evitando el uso de técnicas laboriosas y solventes orgánicos tóxicos

  7. Baroreflexes of the rat. IV. ADN-evoked responses at the NTS.

    Science.gov (United States)

    Tang, Xiaorui; Dworkin, Barry R

    2007-12-01

    In a long-term (7-21 days) neuromuscular blocked (NMB) rat preparation, using precise single-pulse aortic depressor nerve (ADN) stimulation and stable chronic evoked response (ER) recordings from the dorsal-medial solitary nucleus (dmNTS), two different response patterns were observed: continuous and discrete. For the continuous pattern, activity began approximately 3 ms after the stimulus and persisted for 45 ms; for the discrete pattern, two complexes were separated by a gap from approximately 17 to 25 ms. The early complex was probably transmitted via A-fibers: it had a low stimulus current threshold and an average conduction velocity (CV) of 0.58-5.5 m/s; the high threshold late (HTL) complex had a CV = 0.26-0.58 m/s. The average stimulus amplitude-ER magnitude transduction curves for the A and HTL complexes were sigmoidal. For individual rats, in the linear range, mean r2 = 0.96 +/- 0.03 for both complexes. The average stimulus amplitude vs. the systolic blood pressure change (delta sBP) transduction curve was also approximately linear; however, for individual rats, the relationship was not consistently reliable: mean r2 = 0.48 +/- 0.19. Approximately 90% of recording sites had respiratory, and 50% had cardiac synchronism. The NMB preparation is useful for studying central baroreflex mechanisms that operate on time scales of days or weeks, such as adaptation and other kinds of neural plasticity.

  8. 球形ADN的吸湿机理%Hygroscopic Mechanism of Spherical ADN

    Institute of Scientific and Technical Information of China (English)

    王婧娜; 胡岚; 张皋; 王克勇; 严蕊; 张婷

    2014-01-01

    用动态吸湿性分析法和扫描电镜研究了球形二硝酰胺铵(ADN)的吸湿过程和吸湿过程中ADN形貌的变化,探讨了ADN的吸湿机理.结果表明,ADN吸湿过程可分为平缓吸湿(Ⅰ),急剧增强吸湿(Ⅱ)和潮解(Ⅲ)三个阶段,Ⅰ、Ⅱ阶段是水蒸汽与ADN固体表面的作用,Ⅲ阶段是水蒸汽与ADN液化膜表面的作用.Ⅰ阶段吸湿过程为多分子层吸附,符合BET理论模型;该阶段水与ADN之间的作用力对ADN吸湿性能起主导作用;Ⅱ阶段吸湿机理可用毛细孔凝聚理论解释,影响该阶段ADN吸湿性能的主要因素是ADN表面的光滑程度.

  9. NC/NG与ADN的相互作用%Interaction of NG/NC with ADN

    Institute of Scientific and Technical Information of China (English)

    张腊莹; 衡淑云; 刘子如; 张皋; 赵凤起; 谭惠民

    2009-01-01

    用高压差示扫描量热法(PDSC),热重-微商热重法(TG-DTG),研究了双基粘合剂(NC/NG体系)与二硝酰胺铵(ADN)之间的相互作用.结果表明,(NC/NG)/ADN体系中NG的分解峰由NC/NG中的207.0 ℃提前至159.8 ℃,在高压下该分解峰温提前至153.6 ℃.NC/ADN的分解峰温比NG/ADN提前了4.8 ℃,而且大部分的ADN没有被NG加速而提前分解,表明NC对ADN的作用比NG更强烈.此外,还用真空安定性试验(VST)评价了NC/NG与ADN的相容性,混合体系的净增放气量大于11 mL,属于严重不相容,证明在90 ℃下NC/NG与ADN之间也存在强烈的相互作用.

  10. Centros Históricos: El auténtico ADN de las Ciudades

    Directory of Open Access Journals (Sweden)

    Antonio Sahady Villanueva

    2004-01-01

    Full Text Available La ciudad no dejará de mutar mientras exista: se expande, se concentra, se densifica, se vacía. Pero es en el centro fundacional donde se pueden advertir los genes de su verdadera identidad. Aun cuando todos los centros históricos occidentales coinciden en ciertos rasgos arquitectónicos y urbanos, independiente de sus coordenadas geográficas, cada uno de ellos carga con un conjunto de huellas esenciales que los hace únicos e irreproducibles. Cierto es que todas las trazas primitivas de las ciudades latinoamericanas arrancan del patrón común que imponen los colonizadores, pero paulatinamente van derivando hacia modelos particulares que terminan por hacerlas perfectamente identificables unas de otras. Así, Quito es claramente diferente a La Habana y Bahía guarda una gran distancia con Lima. Santiago, por su parte, todavía preserva algunos de los atributos que, en su esplendor -a finales del siglo XIX-, otorgaron a su centro histórico algún grado de unidad. Hoy día las amenazas son múltiples, en tiempos en que la globalización procura sentar una marca anónima y universal. La misión de los profesionales responsables de modificar la ciudad –y de incidir en el centro histórico, por lo tanto- es defender sus vestigios originales, que son los que constituyen en definitiva, su verdadero ADN.

  11. 稳定剂对ADN和NC初期相互作用的影响%Influences of Stabilizers on the Nascent Interaction between ADN and NC

    Institute of Scientific and Technical Information of China (English)

    李吉祯; 王祎; 刘芳莉; 付小龙; 樊学忠; 张腊莹; 王琼

    2011-01-01

    在研究二硝酰胺铵(ADN)的热行为特性及其与硝化纤维素(NC)相互作用的基础上,利用差示扫描量热法(DSC)研究了稳定剂N-甲基对硝基苯胺(MNA)、二号中定剂(C2)、2-硝基二苯胺(2-NDPA)、六次甲基四胺(HMT)及其复配体系(MNA/C2、MNA/2-NDPA、MNA/HMT)对ADN与NC之间初期相互作用的影响.结果表明,稳定剂MNA和C2可使ADN与NC之间的相互作用得到一定程度的减弱,MNA与C2、2-NDPA和HMT的复配协同作用对ADN与NC之间的初期相互作用产生了较为明显的抑制作用,与NC/ADN二元混合体系相比,NC/(ADN/稳定剂)体系的DSC峰温提前量可由19.2℃缩减至11.9℃.%On the basis of the study of thermal behavior of ammonium dinitramide (ADN) and the interaction between ADN and NC, the influences of stabilizers, such as N-methyl-p-nitroaniline(MNA), 1,3-dimethyl-1,3-diphenylurea(C2 ) ,2-nitrodianiline(2-NDPA) ,hexamethylenetetramine(HMT) and its complex MNA/C2, MNA/2-NDPA and MNA/HMT, on the nascent interactions between ADN and NC were investigated by using the method of DSC.The results show that the interaction between ADN and NC can be decreased to a certain extent by mixing MNA and C2, and the nascent interaction between ADN and NC can be inhibited obviously by mixing the complex of MNA/C2, MNA/2-NDPA and MNA/HMT. Comparing the binary system of ADN/NC, the value of △Tp,which is the DSC exothermic peak temperature different between ADN/stabilizer and NC, can be decreased from 19. 2℃ to 11.9 ℃ by using the mixtures of stabilizers.

  12. Preparation and Performaces of ADN/Fe2O3 Nanocomposite Oxidant%ADN/Fe2O3纳米复合氧化剂的制备及性能

    Institute of Scientific and Technical Information of China (English)

    宋肯彭; 宋小兰; 张树海

    2011-01-01

    By using 1,2-epoxypropane as the agent for speeding up the hydrolyzation of Fe(Ⅲ) ions, AND/Fe2O3 composite wet gel was prepared by sol-gel method under a mild and nontoxic condition. Consequently, the AND/ Fe2O3 aerogel was obtained by means of supercritical CO2 fluid extraction. TEM,SEM,EDS,IR,DSC and sensitivity test were employed to characterize the appearance, structure, thermal behaviour and mechanical sensitivity of the samples. The results indicate that the as-prepared AND/Fe2O3 nanoparticles are about 50- 100 nm in diameter. As the decrease of the mass of AND,the nanocomposites become much insensitive to mechanical stimuli. Moreover,the AND/Fe2 O3 nanoparticles decomposes at lower temperature with the disappearance of melting endothermic peak.%采用溶胶-凝胶法,以1,2-环氧丙烷作为Fe(Ⅲ)离子的水解促进剂,在温和无毒的条件下制备了ADN/Fe2O3复合湿凝胶,再将复合湿凝胶进行超临界CO2萃取后,成功获得ADN/Fe2O3气凝胶.用TEM、SEM、EDS、IR DSC和感度试验对上述样品的形貌、结构、热行为和机械感度进行了表征.结果表明,制得的ADN/Fe2O3纳米复合氧化剂的粒径为50~10 nm,撞击感度和摩擦感度均随着其中ADN含量的减小而降低,分解放热峰较原料ADN有所提前,熔化吸热峰消失.

  13. Optimización de un protocolo de extracción de ADN genómico para Pinus tecunumanii

    Directory of Open Access Journals (Sweden)

    David A. Cerda-Granados

    2013-05-01

    Full Text Available Se optimizó un protocolo para la extracción de ADN genómico de Pinus tecunumanii basado en el método de extracción de Doyle y Doyle (1990. Se probaron los megagametofitos de las semillas de árboles deP. tecunumanii muestreados en cinco poblaciones naturales de Nicaragua. El método consta de maceración del tejido en tubos Eppendorf, una extracción con bromuro trimetil amonio de cetilo (CTABempleando altas concentraciones de sales, proteinasa K, extracciones sucesivas con cloroformo-alcohol isoamílico y un tratamiento con ARNasa A. El rendimiento fue aproximadamente 13μg de ADN por 58.7 mg de tejido inicial fresco. El ADNgenómico obtenido por este método fue apropiado para ser usado en reaccionesRAPD (ADN polimórfico amplificado al azar

  14. Comparación de métodos de extracción de ADN en tejidos parafinados y utilidad para amplificación por PCR

    OpenAIRE

    Javier Alonso Baena Del Valle; Alvaro José Ramos Moreno; Claudio Jaime Gómez Alegria; Doris Esther Gómez-Camargo

    2013-01-01

    ResumenLos tejidos fijados en formol e incluidos en parafina son una fuente de material para hallazgos moleculares en el ámbito clínico y científico, demostrándose que el ADN extraído de éstos,  es adecuado para amplificación a través de la reacción en cadena de la polimerasa (RCP). En este estudio, se ensayaron tres métodos de extracción de ADN en tejidos incluidos en parafina, con el objetivo de comparar la eficiencia de estos para obtener ADN adecuado, además se analizó su utilidad en ampl...

  15. 汽车设计网络(ADN):标致-雪铁龙的新标志

    Institute of Scientific and Technical Information of China (English)

    蒋东镭

    2005-01-01

    标致-雪铁龙集团设计中心又被称为汽车设计网络(以下简称为ADN),集团认为这个名字在观念上符合了设计中心的本质——个完全致力于创新和汽车革新的场所。缩写的ADN在法语中拼为DNA,则表达了该集团要恢复标致和雪铁龙这两个品牌的传统特性的用意。

  16. La aplicación de los análisis de ADN en el proceso penal

    OpenAIRE

    Hernández Hernández, Edmundo

    2007-01-01

    Resulta difícil para una persona experta resumir en unas breves líneas lo que ha supuesto el extraordinario descubrimiento científico que gira en torno al ADN. De su actualidad nos sobrada cuenta las constantes referencias en los medios de comunicación, con frecuencia acompañada de predicciones futuras próximas a la ciencia ficción. Su repercusión desde plano de ética resulte igualmente innegable pues, al igual que la gran mayoría de los descubrimientos científicos más relevantes, las posibil...

  17. Determinación de la efectividad de cinco protocolos de extracción de ADN a partir de material parafinado para estudios moleculares

    OpenAIRE

    Jiménez Arce, Gerardo; Villalobos Quesada, María José; Jiménez Montero, Ernesto; Palma Platero, Walter

    2007-01-01

    El estudio evaluó cuatro distintos protocolos de uso común en laboratorio y uno comercial, para la extracción de ADN a partir de material fijado en formalina y parafinado, correspondiente a cervix uterinos producto de conizaciones y LEEPs (loop electrocautery excision procedure), provenientes de los Servicios de Patología de nueve Hospitales de la Caja Costarricense de Seguro Social (CCSS). Se encontró que el protocolo más efectivo para obtener ADN viable para la PCR, es el basado en extracci...

  18. Cuantificación de ADN libre en plasma sanguíneo de voluntarios sanos en una población bogotana

    OpenAIRE

    Hernán David Salazar-Jordan et al.

    2009-01-01

    El ADN libre en sangre incrementa con algunas condiciones patológicas y ciertos estados fisiológicos. Varios reportes en la literatura han resaltado que el ADN libre en plasma o suero tiene potencial clínico como una posible herramienta para el pronóstico de cáncer en humanos. Sin embargo, hasta el momento no se tienen valores de referencia de individuos sanos con un tamaño de muestra representativo y tampoco se han descrito valores para poblaciones específicas como la bogotana. Es por ello q...

  19. Método para la replicación, amplificación o secuenciación de un ADN molde

    OpenAIRE

    2009-01-01

    Método para la replicación, amplificación o secuenciación de un ADN molde. La presente invención se encuadra dentro del campo de la biotecnología. Específicamente, se refiere a un método para llevar a cabo la replicación, la amplificación o la secuenciación de un ácido desoxirribonucleico con una ADN polimerasa del tipo 29 y a un kit para llevar a cabo dicho método.

  20. Estrategias para mejorar la comprensión del proceso de replicación del ADN en alumnos de la Escuela Secundaria

    OpenAIRE

    2014-01-01

    Teniendo como hipótesis que la representación gráfica de la replicación del ADN utilizada habitualmente genera una comprensión inadecuada del proceso, y que la utilización de una representación alternativa que sitúa las horquillas en el interior de una o varias burbujas de replicación mejora la comprensión del proceso, se presenta una secuencia de estrategias de enseñanza con el objetivo de contribuir a la mejor comprensión del proceso de replicación del ADN Bearing the hypothesis that the...

  1. Caracterización múltiple de la fragmentación del adn espermático en pacientes infértiles

    OpenAIRE

    García Peiró, Agustín

    2013-01-01

    La infertilidad es un problema que afecta al 15 % de las parejas en edad reproductiva. Así pues, el análisis de los factores que impiden obtener un embarazo es un tema de interés creciente Se considera que la infertilidad se debe a un factor masculino en el 30-50% de los casos. Recientemente, varios estudios han demostrado que la integridad del ADN espermático es un factor importante para la correcta transmisión de la información genética paterna. Daños en el ADN podrían afectar tanto a la fe...

  2. Comparación de tres métodos de extracción de ADN a partir de restos óseos

    OpenAIRE

    Del Valle, Christian; Rodríguez, Anayanci; Espinoza, Marta

    2014-01-01

    Diferentes investigaciones han demostrado que la posibilidad de llevar a cabo estudios de marcadores genéticos está muchas veces limitada por la capacidad de obtener ácidos desoxirribonucleicos (ADN) a partir de muestras degradadas como los restos óseos. Asu vez, la información que de allí se derive resulta de suma importancia en diferentes situaciones médico-legales. El presente estudio se diseñó para comparar la eficiencia de tres métodos orgánicos de extracción de ADN amplificable por la t...

  3. OPTIMIZACIÓN DE LOS PROTOCOLOS DE EXTRACCIÓN DE ADN Y DEL MARCADOR MOLECULAR TIPO RAPD EN ANONÁCEAS

    OpenAIRE

    Yanet Alfonso Alonso; Caridad Noriega Carrera; Miriam Isidrón Pérez; Lucy Andraca Collazo; Dubiel Alfonso González; Daymara Rodríguez Alfonso

    2016-01-01

    Las técnicas moleculares requieren de protocolos que permitan determinar los niveles de variación genética, dentro de las poblaciones en diferentes condiciones ambientales. Tanto la optimización del aislamiento del ADN, como el de las condiciones de trabajo de las amplificaciones, son fundamentales para alcanzar el éxito de los análisis moleculares, por lo que la presente investigación tiene como objetivo: optimizar los protocolos de extracción de ADN y del marcador molecular tipo RAPD (Rando...

  4. Differences in stress and challenge in clinical practice among ADN and BSN students in varying clinical courses.

    Science.gov (United States)

    Oermann, M H; Standfest, K M

    1997-05-01

    There is limited research on student perceptions of their clinical experiences particularly in different clinical courses and settings. The purpose of this study was to examine the degree of stress, challenge, and threat associated with clinical practice experiences among ADN and BSN nursing students enrolled in various clinical courses and settings. The Pagana Clinical Stress Questionnaire was used to assess the clinical experiences of 416 undergraduate nursing students from 10 randomly selected associate degree (ADN) and baccalaureate (BSN) programs in the midwest. Significant differences were found across clinical courses (F[5,399] = 9.58, p < .0001) and settings (F[2,386] = 4.63, p = .01) in terms of students' stress. Stress was highest for students enrolled in pediatric nursing courses. ANOVA indicated significant differences across clinical courses in the degree of challenge and threat experienced by students. There were no differences in any of the variables studied when students had faculty from the nursing program or preceptors from the clinical setting as their teachers.

  5. Interaction of ADN with nitrate oxidizer%ADN与硝胺氧化剂的相互作用

    Institute of Scientific and Technical Information of China (English)

    张腊莹; 刘子如; 衡淑云; 韩芳; 岳璞

    2007-01-01

    用高压差示扫描量热法(PDSC)和热重-微商热重法(TG-DTG),研究了高能氧化剂ADN(二硝酰胺铵)的热分解及其与HMX和RDX的相互作用.结果表明,ADN与HMX和RDX之间存在着强烈的相互作用.因部分HMX溶于熔融的ADN中,而参与了ADN组分的分解,ADN的放热分解峰温因压力升高而提高,而HMX产生了双分解峰.大量的RDX因ADN的熔融而提前液化与ADN一起分解,因ADN气相产物的抑制作用,使混合体系中RDX组分常压下的分解峰温后移,而RDX自身分解气相产物的催化作用,使其高压下的分解峰温前移.

  6. - Las vacunas de ADN: una promisoria medicina para el paciente veterinario (DNA vaccines: a promising medicine for the veterinary patient

    Directory of Open Access Journals (Sweden)

    Juan Carlos Díaz David

    2006-02-01

    Full Text Available Resumen. Las vacunas de ADN constituyen una promisoria herramienta en vacunología moderna. Al tratarse de una tecnología fácil de aplicar y de gran versatilidad, capaz de estimular una respuesta inmune humoral y celular, esenciales en la lucha contra infecciones virales, constituye una línea primordial de investigación y desarrollo. Esta revisión aborda las características de un vector de ADN y los mecanismos propuestos para la generación de la respuesta inmune mediante este tipo de vacunación. Igualmente, se discuten algunos regímenes de vacunación, ejemplos de respuestas inmunes protectoras obtenidas en especies de interés veterinario, y se hace referencia a las cuestiones de inocuidad inherentes a este tipo de vacuna. Abstract. DNA vaccines represent an invaluable tool in modern vaccinology. Besides being a simple versatile technology, capable of stimulating both cellular and humoral immune responses, it is also an essential weapon to fight against infections of viral etiology. This review emphasizes the characteristics of a DNA vaccine vector, as well as the proposed mechanisms responsible for the generation of a protective immune response. Furthermore, immunization regimes are discussed, examples of protective immune responses attained in target species of veterinary interest are given and reference is made to the safety concerns derived from this kind of vaccine.

  7. Comparación de métodos de extracción de ADN en tejidos parafinados y utilidad para amplificación por PCR

    Directory of Open Access Journals (Sweden)

    Javier Alonso Baena Del Valle

    2013-06-01

    Full Text Available ResumenLos tejidos fijados en formol e incluidos en parafina son una fuente de material para hallazgos moleculares en el ámbito clínico y científico, demostrándose que el ADN extraído de éstos,  es adecuado para amplificación a través de la reacción en cadena de la polimerasa (RCP. En este estudio, se ensayaron tres métodos de extracción de ADN en tejidos incluidos en parafina, con el objetivo de comparar la eficiencia de estos para obtener ADN adecuado, además se analizó su utilidad en amplificación por RCP. Se emplearon tres muestras, correspondientes a una biopsia de pulmón, legrado endometrial y ganglio linfático, todas fijadas en formaldehido al 10% e incluidas en parafina. Utilizándose tres métodos diferentes de extracción de ADN (extracción por salting out, método modificado de Sambrook y kit comercial El ADN obtenido se cuantificó por espectrofotometría, además se realizó electroforesis en gel de agarosa al 1%, para comprobar si el ADN era de buena calidad y se  realizó RCP para el exón 3 del gen caveolina 1. Todos los métodos dieron como resultado una buen producto de ADN genómico, observándose mayor cantidad y pureza en los métodos de salting out y kit comercial, asimismo se obtuvo amplificación del producto esperado por estos dos métodos, no hubo buenos resultados con el ADN extraído por el método modificado "Preparation of Genomic DNA from Mouse Tails y Other Small samples, según Sambrook”. El ADN obtenido a partir de tejidos FFIP puede ser amplificado por varios métodos, entre estos, la extracción por salting out es útil y con poca toxicidad,  permite obtener ADN de buena calidad para amplificación por RCP. Abstract Formalin-fixed and paraffin-embedded tissues are a source of important molecular findings in clinical and scientific, demonstrating that the DNA extracted from these is suitable for amplification by polymerase chain reaction (PCR. In this study, we tested three methods of

  8. ADN-1184, a monoaminergic ligand with 5-HT6/7 receptor antagonist action, exhibits activity in animal models of anxiety.

    Science.gov (United States)

    Partyka, Anna; Wasik, Anna; Jastrzębska-Więsek, Magdalena; Mierzejewski, Paweł; Bieńkowski, Przemysław; Kołaczkowski, Marcin; Wesołowska, Anna

    2016-06-01

    Behavioral and psychological symptoms of dementia (BPSD) include apathy, sleep problems, irritability, wandering, elation, agitation/aggression, and mood disorders such as depression and/or anxiety. Elderly patients are usually treated with second-generation antipsychotics; however, they present not enough efficacy against all symptoms observed. Hence, there still is an unmet need for novel pharmacotherapeutic agents targeted BPSD. A novel arylsulfonamide derivative ADN-1184 has been developed that possesses a preclinical profile of activity corresponding to criteria required for treatment of both psychosis and depressive symptoms of BPSD without exacerbating cognitive impairment or inducing motor disturbances. To broaden its pharmacological efficacy toward anxiety symptoms, its anxiolytic properties have been examined in common animal preclinical models in rats and mice. ADN-1184 significantly increased the number of entries into open arms measured in the elevated plus-maze test; however, it simultaneously increased parameters of exploratory activity. In the Vogel conflict drinking test, ADN-1184 dose-dependently and significantly increased the number of shocks accepted and the number of licks. Moreover, in mice, it also had specific anxiolytic-like activity in the four-plate test, and only negligible one at a specific mid-range dose measured in the spontaneous marble burying test. The obtained findings reveal that ADN-1184 displays anxiolytic-like activity in animal models of anxiety which employed punished stimuli. In its unusual combination of some anxiolytic action with already proven antipsychotic and antidepressant properties, and lack of any disruptive impact on learning and memory processes and motor coordination, ADN-1184 displays a profile that would be desired for a novel therapeutic for BPSD.

  9. Secuencia parcial de un fragmento de ADN de patos silvestres homologo al Complejo Mayor de

    Directory of Open Access Journals (Sweden)

    González-Guzmán Sofía

    2009-04-01

    Full Text Available ResumenLa variación individual de la respuesta inmune esta ligada a ladiversidad funcional y estructural del MHC (por sus siglas en inglesMajor Histocompatibility Complex, TcR (Receptores de células T,inmunoglobulinas (anticuerpos, citoquininas, TNF (por sus siglas eningles Tumor Necrosis Factor, entre otras proteínas. Estas proteínasestán codificadas por múltiples y polimórficos genes, la variabilidad de estos genes constituyen las principales bases, en las variaciones de la resistencia a determinada enfermedad o enfermedades. Estascaracterísticas se han documentado en la gallina doméstica, pero no enel pato. En el presente trabajo se amplifico por PCR un fragmento denucleótidos de ADN genómico de pato doméstico que compartió un100% de similitud con un fragmento del MHC clase II de pollo, quecorrespondió a una secuencia similar a DAB1 (Disabled 1.La secuencia DAB1 también fue amplificada en nueve especies de patos silvestres del género Anas que posterior a un análisis por el polimorfismo del largo de los fragmentos de restricción (PLFR con las enzimas RsaI, BsrI y HindIII, se observó que la especie Anas americana,Anas platyrhynchos diazi y Anas platyrhynchos domesticus fueronsutilmente diferentes a la especie Anas discors, Anas crecca, Anascyanoptera, Anas acuta, Anas streptera y Anas clypeata. Hasta dondesabemos, este es el primer reporte que indica la presencia desecuencias similares a DAB1 en patos silvestres. Este estudio podríaayudar a entender porque los patos podrían ser menos susceptibles apresentar neoplasias que en las gallinas.SummaryThe individual response of the immune system is linked to the functional and structural diversity of the MHC (Major Histocompatibility Complex, TcR (T cell receptor, inmunoglobulines (antibodies, citokines, TNF (Tumor Necrosis Factor, among other proteins. These proteins are coded for multiple and polymorphic genes, the variability of these genes constitutes the main bases, in the

  10. Modelado molecular de la interacción de fármacos antitumorales y nucleasas con el ADN

    OpenAIRE

    Bueren Calabuig, Juan Antonio

    2011-01-01

    Premio Extraordinario de Doctorado de la UAH en 2013 Los métodos computacionales están convirtiéndose en herramientas muy importantes en ciertas áreas de la investigación como la caracterización de sitios de unión de ligandos a proteínas, acoplamiento de pequeñas moléculas en sitios de unión al ADN y proteínas y simulaciones de dinámica molecular. Los resultados obtenidos aportan información que, a veces, está más allá de las posibilidades puramente experimentales y pueden usarse para gui...

  11. El ADN de la marca. La concepción de sus valores intangibles en un contexto dialogado

    Directory of Open Access Journals (Sweden)

    Jaime Alberto Orozco Toro

    2012-01-01

    Full Text Available Aún hoy, en plena sociedad del conocimiento, se tienen límites conceptuales en relación con aspectos tan fundamentales como la identidad, la imagen y la reputación de marca. Incluso donde mayores dificultades se presentan es en las posibilidades de evaluación y medición de cada uno de estos conceptos ligados con la reputación empresarial. Así, la marca, como el elemento fundamental de las empresas del presente siglo, se ve obligada a indagar nuevas formas de interpretar sus acciones estratégicas, con el único objetivo de continuar conformando la estructura de la marca, el ADN que la diferencia y que le permite contrastar sus resultados a partir de evaluaciones y mediciones fiables, en un contexto donde la transparencia, ante el alud de comunicación, se plantea casi como una exigencia.

  12. Comparación de dos métodos de extracción de adn a partir de plantas del género solanum, subgénero leptostemonum.

    OpenAIRE

    2013-01-01

    Título en ingles: Comparison of two ADN extraction methods from plants belonging Solanum genus Leptostemonumsubgenus.Título corto: Extracción de ADN de SolanumResumen: Se evaluaron dos métodos para la extracción de ADN en plantas del género Solanum, con el fin de obtener ADN disponible y de buena calidad para la obtención de secuencias. El producto comercial DNeasy® Plant Mini Kit se comparó con un método que incluye el uso de una solución tampón de lisis. Para este último método también se e...

  13. Extracción y purificación de ADN a partir de muestras de saliva: Un avance diagnóstico en el paciente pediátrico

    OpenAIRE

    Hernández Frías, Olaya

    2014-01-01

    El objetivo general de este trabajo es la puesta a punto en la práctica diaria del laboratorio de un nuevo método de obtención de ADN a partir de muestras de saliva. Para ello se pondrá de manifiesto su rendimiento, valorando sus ventajas e inconvenientes respecto al método que tradicionalmente está siendo empleado, la extracción de ADN a partir de muestras de sangre.

  14. Estandarización de un protocolo sencillo para la extracción de adn genómico de levaduras

    OpenAIRE

    2009-01-01

    Standardising a simple protocol for extracting yeast from genomic DNA Resumen: Se estandarizó un protocolo rápido, sencillo y de bajo costo para la extracción de ADN genómico de levaduras a partir de lisis de la pared celular mediante tratamiento enzimático y precipitación por alcoholes. El empleo de la enzima Beta-glucoronidasa en reemplazo de la enzima Zimolasa, permitió obtener ADN en alta concentración (124,9±30,2 ng/μl) y de buena calidad (A260/A280 nm =1,86±0,1), ideal para su uso en e...

  15. ADN无机法合成及分离纯化研究%Inorganic Synthesis of ADN and Its Separation and Purification

    Institute of Scientific and Technical Information of China (English)

    刘愆; 王伯周; 张海昊; 廉鹏; 王锡杰; 贾思媛

    2006-01-01

    以氨基磺酸为起始原料,经氢氧化钾中和获得氨基磺酸钾,然后用硝硫混酸低温硝化、氨气中和合成了二硝酰胺铵盐(ADN),初步探索了硝化反应温度对收率的影响.采用活性炭吸附的方法对ADN进行了有效的分离纯化,对活性炭用量、洗脱速度、洗脱终点、解吸方式等进行了研究,获得纯度为99.8%的ADN.

  16. 混酸法合成ADN的分离纯化工艺研究%Separation and Purification of ADN Synthesized by Mixed Acid Method

    Institute of Scientific and Technical Information of China (English)

    刘海洲; 许华新; 李宁; 王学敏; 陈金中

    2009-01-01

    二硝酰胺铵(ADN)是一种高能高燃速和不含氯元素的新型氧化剂,混酸法合成ADN时会生成大量的无机盐副产物,采用活性炭吸附的方法可以对ADN进行有效的分离纯化.对活性炭的种类、吸附溶剂的种类、解吸溶剂的利类、吸附浓度、吸附方式进行了研究,并确定了较佳的工艺条件,制备了质最分数达99.5%以上的ADN.

  17. 聚氨酯黏合剂包覆球形ADN的性能研究%Properties of Prilled Ammonium Dinitramide (ADN)Coated by Polyurethane Binders

    Institute of Scientific and Technical Information of China (English)

    胥会祥; 廖林泉; 刘愆; 李勇宏; 冉秀伦; 赵凤起

    2008-01-01

    为降低球形二硝酰胺铵(ADN)的吸湿性,采用聚氨酯黏合剂对其进行包覆.用扫描电镜(SEM)和称重法分析了包覆后ADN的表面形貌和吸湿性,结果表明,聚氨酯黏合剂在ADN表面形成絮状的包覆膜,使包覆后ADN的吸湿率明显小于包覆前ADN,在相对湿度60%以下,包覆后ADN在空气中暴露30天时吸湿率仅0.136%,吸湿率不随时间增加.热分析和机械感度测试表明,聚氨酯黏合剂对ADN的熔点、分解峰温、分解热焓的影响较小,但使ADN的机械感度增加,包覆前后ADN的摩擦感度分别为30%和60%,撞击感度(H50)分别为30.5 cm和27.8 cm.

  18. Nuclear DNA content in Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae Contenido de ADN nuclear en Galaxias maculatus (Teleostei: Osmeriformes: Galaxiidae

    Directory of Open Access Journals (Sweden)

    Pedro Jara-Seguel

    2008-01-01

    Full Text Available The nuclear DNA content (2C value was determined in the commercial fish Galaxias maculatus (Galaxiidae was determined by microdensitometry of erythrocyte nuclei after Feulgen staining; rainbow trout erythrocytes with a known 2C value were used as a standard. The 2C value of G. maculatus was 2.21 ± 0.12 pg and its C value was equivalent to 1.105 pg (1,082.9 Mbp. This C value is within the range recorded for other osmeriform species (0.62-3.2 pg. The average sperm head diameter of G. maculatus is lower than the average sperm head diameter of rainbow trout (used as a standard, which coincides with the differences observed in the nuclear DNA content of both species. This information increases the genome data available for G. maculatus and might be useful in future programs dealing with its genetic manipulation.El contenido de ADN nuclear (valor 2C fue determinado en el pez comercial Galaxias maculatus (Galaxiidae usando microdensitometría de núcleos de eritrocitos sometidos a tinción de Feulgen, utilizando como estándar eritrocitos de trucha arco iris con un valor 2C conocido. El valor 2C de G. maculatus fue 2,21 ± 0,12 pg y su valor C es equivalente a 1,105 pg (1.082,9 pMb. Este valor C está dentro del rango registrado para otras especies de osmeriformes (0,62-3,2 pg. El diámetro promedio de la cabeza del espermatozoide de G. maculatus es menor al promedio descrito para la trucha arco iris utilizado como estándar, lo que coincide con las diferencias observadas en el contenido de ADN nuclear entre ambas especies. Estos datos contribuyen a ampliar los antecedentes genómicos disponibles para G. maculatus y podrían ser útiles en futuros programas tendientes a su manipulación genética.

  19. Diversidad genética de poblaciones de ajo (Allium sativum L. cultivadas en Guatemala, definida por marcadores de ADN

    Directory of Open Access Journals (Sweden)

    Fredy Uber Rosales-Longo

    2007-01-01

    Full Text Available Diversidad genética de las poblaciones de ajo (Allium sativum L. cultivadas en Gua temala, definida por mar cado res de ADN. En Guatemala es escasa la in for ma ción sobre la diversidad genética de ajo. Los objetivos del estudio fueron: incidir en el mejoramiento de Allium sativum, so bre la base del conocimiento de su variabilidad genética, así mismo, establecer una colección in vi tro de la co lec ción de las poblaciones cultivadas en Guatemala. Los experimentos fueron realizados entre octubre de 2005 y marzo de 2006. La determinación de las variaciones de ADN se realizaron me dian te la téc ni ca de AFLP™. La información se analiza por medio de análisis de componentes principales, análisis de coordenadas principales y análisis de conglomerados. Mediante la inspección de los pro duc tos de AFLP™ y análi sis estadísticos, se detectó una alta variabilidad genética entre los materiales vegetales colectados. Las muestras clasificadas co mo del ti po “Chi leno”, correspondieron a los tipos “Criollo”. Nueve bien diferenciados grupos genéticos se conformaron en un dendrograma y se con fir mó que la diversidad genética descubierta es una función del lugar don de se cul ti van las po bla cio nes de ajo. Se identificó una mayor diversidad genética entre las mues tras de ajo del ti po “Crio llo” que las que se tienen en tre los ma te ria les del ti po “Chileno”, como producto de la mayor dispersión espacial de los primeros. Los materiales genéticos de ajo se encuentran actualmente preservados en un Banco de Germoplasma in vi tro en la Uni dad de Bio tec no lo gía del IC TA.

  20. Estandarización de un protocolo sencillo para la extracción de ADN genómico de levaduras

    Directory of Open Access Journals (Sweden)

    Esteban Osorio-Cadavid

    2009-10-01

    Full Text Available Standardising a simple protocol for extracting yeast from genomic DNA Resumen: Se estandarizó un protocolo rápido, sencillo y de bajo costo para la extracción de ADN genómico de levaduras a partir de lisis de la pared celular mediante tratamiento enzimático y precipitación por alcoholes. El empleo de la enzima Beta-glucoronidasa en reemplazo de la enzima Zimolasa, permitió obtener ADN en alta concentración (124,9±30,2 ng/μl y de buena calidad (A260/A280 nm =1,86±0,1, ideal para su uso en estudios de biología molecular. Además, se adicionó un paso de incubación del ADN obtenido a 100° C para inactivar ADNasas. La calidad del ADN obtenido fue evaluada por medio de la amplificación de la región ITS1-5.8S-ITS2, presentando bandas definidas y cuantificables (entre 380 y 880 pb ideales para estudios de identificación molecular y filogenia.Palabras clave: levadura; extracción de ADN; Beta-Glucoronidasa. Abstract: A quick, simple and low-cost protocol for extracting genomic DNA from yeast by cell wall lysis involving enzymatic treatment and alcoholic precipitation was standardised. Higher DNA yields (124.9±30.2 ng/μl were obtained by using beta-glucuronidase instead of zymolyase; these had very high quality (A260/A280 nm = 1.86±0.1 and would be suitable for use in molecular biology assays. Moreover, a DNAse inactivation step was also introduced by incubation at 100 °C to further ensure DNA stability. DNA quality was assayed by PCR amplification of the ITS1-5.8S-ITS2 region, revealing defined, quantifiable 380 to 880 bp bands. These results show that the protocol is ideal for molecular identification and phylogenetic studies.Key words: Yeast; DNA extraction; beta-glucoronidase.

  1. Hygroscopicity of ADN with Dynamic Method%动态法研究ADN的吸湿性能

    Institute of Scientific and Technical Information of China (English)

    王婧娜; 张皋; 严蕊; 胡岚; 张婷

    2012-01-01

    针对二硝酰胺铵(ADN)的强吸湿性及吸湿性研究的迫切性,建立了动态分析的新方法.采用快速分析方法研究了ADN的吸湿性能,得出了ADN的吸湿过程曲线、临界相对湿度、吸湿速率曲线.比较动态分析方法与干燥器平衡法(标准方法GJB7708 -2005 -404.1)结果,认为两种方法准确度等效.动态分析方法的相对标准偏差为1.45%.%Aim at the strong hygroscopicity of ammonium dinitramid( AND)and the necessity of the hygroscopicity investigation,a new dynamic analytical method was estabilished. The hygroscopicity of AND was studied by a rapid analytical method. The curves describing the hygroscopic process, hygroscopic speed and critical relative humidity were obtained. Compared to airer equity method, the dynamic method showed an equivalent result to the standardization method GJB770B -2005 -404. 1 in accuracy. The relative standard deviation of the dynamic analytical method was 1.45%.

  2. El entramado filiatorio y el ADN: Análisis de las competencias profesionales del psicólogo

    Directory of Open Access Journals (Sweden)

    Natacha Salomé Lima

    2016-03-01

    Full Text Available El presente trabajo se propone evaluar el grado de conocimiento que tienen los profesionales psicólogos en el ámbito de dos situaciones filiatorias controvertidas: casos de restitución de identidad vs casos de niños concebidos por tecnologías de reproducción humana asistida (TRHA, estableciendo como eje vertebrador al ADN y sus múltiples significaciones. ¿Ofrece la formación de grado los contenidos apropiados para desarrollar el quehacer profesional a la luz de las nuevas demandas sociales y tecno-científicas? Se presenta el análisis de los datos cuali-cuantitativos obtenidos a partir de una muestra conformada por 76 entrevistas realizadas a profesionales psicólogos, de ambos sexos, cuya edad oscila entre los 23 y los 70 años, de los cuales el 86 % han sido formados con el mismo plan de estudios de la Facultad de Psicología de la Universidad de Buenos Aires, Argentina. En las conclusiones se destaca la falta de ajuste existente entre las nuevas necesidades sociales y la capacitación que los graduados reciben en el caso particular de la República Argentina.

  3. EVALUACIÓN DE DOS MÉTODOS DE EXTRACCIÓN DE ADN A PARTIR DE BIOPSIAS FIJADAS EN FORMALINA Y EMBEBIDAS EN PARAFINA EN CONDICIONES NO ÓPTIMAS

    Directory of Open Access Journals (Sweden)

    JAVIER ANDRÉS BUSTAMANTE

    2011-01-01

    Full Text Available Los tejidos de archivo son material de incalculable valor para estudios retrospectivos que requieran la aplicación de análisis moleculares. Existen múltiples métodos de extracción de ADN a partir de este tipo de muestras. No obstante, la mayoría de mé- todos toman mucho tiempo y los reactivos empleados contribuyen a la fragmentación del ADN. Con el objetivo de optimizar dos métodos de extracción de ADN a partir de tejidos embebidos en parafina en condiciones no óptimas, se seleccionaron 47 bloques en parafina que contenían biopsias de pleura, pulmón y pericardio correspondientes a 24 pacientes (66,6% hombres mayores de 18 años, con inflamación granulomatosa crónica, remitidos al Departamento de Patología, Hospital Universitario del Valle entre 2002 y 2007. Se realizaron 10 cortes a cada muestra y se sometieron a dos métodos de extracción de ADN: 1. convencional y 2. QIAamp-DNA mini kit ® . La eficiencia del ADN fue valorada por espectrofotometría y amplificación del gen GAPDH. La concentración de ADN de las muestras extraídas por el método convencional fue de 65,52 ng/μL ±11,47 (promedio ± EE y la relación 260/280 varió entre 0,52 y 2,30. De las muestras extraídas por el método comercial, la concentración media de ADN fue 60,89 ng/μL ± 6,02, con una absorbancia que osciló entre 0 y 2,64. El ADN obtenido fue sometido a PCR, de 47 muestras extraídas por ambos métodos, 25 y 23 respectivamente amplificaron exitosamente el gen GAPDH. Los métodos usados para la obtención de ADN presentaron un desempeño similar, revelando así su potencial utilidad en estudios retrospectivos a partir de biopsias embebidas en parafina en condiciones inadecuadas.

  4. Evaluación de dos métodos de extracción de ADN a partir de biopsias fijadas en formalina y embebidas en parafina en condiciones no optimas

    Directory of Open Access Journals (Sweden)

    Bravo Luis Eduardo

    2011-08-01

    Full Text Available Los tejidos de archivo son un material de incalculable valor para estudios retrospectivos que requieran la aplicación de análisis moleculares. Existen múltiples métodos de extracción de ADN a partir de este tipo de muestras. No obstante, la mayoría de m��todos toman mucho tiempo y los reactivos empleados contribuyen a la fragmentación del ADN. Con el objetivo de optimizar dos métodos de extracción de ADN a partir de tejidos embebidos en parafina en condiciones no optimas. Se seleccionaron 47 bloques en parafina que contenían biopsias de pleura, pulmón y pericardio correspondientes a 24 pacientes (66,66%hombres mayores de 18 años, con inflamación granulomatosa crónica, remitidos al Departamento de Patología del Hospital Universitario del Valle entre 2002-2007. A cada muestra se le realizaron 10 cortes y se sometieron a dos métodos de extracción de ADN: 1.Convencional y 2.QIAamp-DNA mini kit®. La eficiencia del ADN fue valorada por espectrofotometría y amplificación del gen GAPDH. La concentración de ADN de las muestras extraídas por el método convencional fue de 65.52ng/µl ±11.47 (promedio±EE y la relación 260/280 vario entre 0.52 y 2.30. De las muestras extraídas por el método comercial, la concentración media de ADN fue 60.89ng/µl±6.02, con una absorbancia que oscilo entre 0 y 2.64. El ADN obtenido fue sometido a PCR, de 47 muestras extraídas por ambos métodos, 25 y 23 respectivamente amplificarón exitosamente el gen GAPDH. Los métodos usados para la obtención de ADN presentaron un desempeño similar, revelando así su potencial utilidad en estudios retrospectivos a partir de biopsias embebidas en parafina en condiciones inadecuadas.

  5. Interaction and compatibility of ADN with compositions of propellant by thermal analysis method%热分析法研究ADN与推进剂组分的相互作用及相容性

    Institute of Scientific and Technical Information of China (English)

    张伟; 邵重斌; 刘芳莉; 樊学忠; 谢五喜; 庞维强; 闫宁; 刘小刚; 刘春; 姬月萍

    2014-01-01

    用高压差示扫描量热法( PDSC),研究了ADN(二硝酰胺铵)与推进剂的粘合剂、固化剂、增塑剂和高能填料的相互作用及相容性。结果表明,ADN与PEG、TDI和HDI二元混合物的分解温度比ADN的分解峰温低16.3~26.9℃,混合体系相互作用危险,相容性差;ADN与NG-BTTN、TEGDN、Bu-NENA和HMX二元混合物的分解温度略高于ADN的分解峰温0.5~7.5℃,对应混合物的分解温度比NG-BTTN、TEGDN、Bu-NENA和HMX的分解温度低5.9~14.4℃,混合体系的相互作用较为敏感;ADN与GAP、PBT、PET、IPDI、N-100、NG、TMETN、BDNPA-F和Al二元混合物的分解温度与各单组分分解温度差小于2℃,上述组分相容。%The interaction and compatibility of ADN with various main compositions of propellant, including binders, curing a-gents, plasticizers and energetic fillers, were investigated by means of PDSC.Results show that Tp(decomposition temperature) of ADN-PEG,-TDI and -HDI dual mixing system is 16.3~26.9℃ lower than Tp of ADN.The interactions between them are dangerous and ADN is incompatible with PEG, TDI and HDI;Tp of ADN-NG/BTTN,-TEGDN,-Bu-NENA and-HMX dual mixing system is 0.5~7.5 ℃ higher than Tp of ADN and 5.9~14.4℃ lower than those of corresponding compositions(NG/BTTN, TEGDN, Bu-NE-NA and HMX), thus the interactions between them are sensitive;ΔTp(difference of decomposition temperature) of ADN-GAP, -PBT,-PET,-IPDI,-N-100,-NG,-TMETN,-BDNPA-F and -Al dual mixing system with the corresponding compositions is less than 2 ℃, and ADN is found to be compatible with compositions mentioned above.

  6. Effect of a new aldose reductase inhibitor, 8'-chloro-2',3'-dihydrospiro [pyrrolidine-3,6'(5'H)-pyrrolo[1,2,3-de] [1,4]benzoxazine]-2,5,5'- trione (ADN-138), on delayed motor nerve conduction velocity in streptozotocin-diabetic rats.

    Science.gov (United States)

    Hirata, Y; Fujimori, S; Okada, K

    1988-02-01

    The effects of a chemically new type of aldose reductase inhibitor, ADN-138, on delayed motor nerve conduction velocity (MNCV) and sciatic nerve sorbitol, fructose and myo-inositol levels were studied in streptozotocin-diabetic rats. MNCV in rats was significantly delayed after 3 weeks of diabetes and ADN-138 treatment was started at this point. Treatment of diabetics with ADN-138 at 5 and 20 but not 1 mg/kg/d for 3 weeks resulted in a significant increase in MNCV and reduced sorbitol levels to or below those of nondiabetic controls. However, fructose, though decreased in a dose-dependent manner, was not normalized. The reference drug, Sorbinil, showed similar effects on them. After the 3 weeks of ADN-138(20 mg/kg/d) treatment, diabetics were left on ADN-138 or continued further to be treated with it for 3 weeks. The withdrawal of ADN-138 prevented a further increase in MNCV and restored sorbitol and fructose to nontreated diabetic levels, and myo-inositol levels declined. In contrast, the ADN-138-continued group kept improving its MNCV and normalized sorbitol and myo-inositol. These results suggest that polyol accumulation is responsible for delayed MNCV and that the action of ADN-138 on MNCV reflected reversibility of metabolic function in diabetics.

  7. Diseño y síntesis de metalopéptidos y su aplicación como agentes de unión al ADN

    OpenAIRE

    Rama Martínez, Gustavo

    2014-01-01

    Uno de los campos más activos en Química Biológica y en Química Médica es el desarrollo de moléculas con utilidad terapéutica, incluyendo las que tienen el ADN como diana. El descubrimiento del origen genético de múltiples enfermedades, así como la influencia del genotipo en la respuesta a medicamentos, hace que la detección de secuencias específicas del ADN tenga un enorme potencial diagnóstico y clínico. Entre los agentes clásicos de reconocimiento específico del ADN desta...

  8. DOMMAGES CAUSÉS À LA MOLECULE D’ADN PAR LES EFFETS CONJUGUÉS DE DEUX DISTRIBUTIONS D’ESPÈCES RADIOLYTIQUES

    Directory of Open Access Journals (Sweden)

    M.E.K ABDELMOUMENE

    2009-06-01

    Full Text Available L’ADN, support de l’information génétique de la cellule, est la cible la plus importante lors d’une exposition à un rayonnement ionisant (radiothérapie, scintigraphie…. Sa dégradation est à l’origine de désordres cellulaires dramatiques comme la cancérisation. Pour quantifier ces effets, nous avons considéré deux électrons d’énergie incidente 1 KeV qui traversent un milieu aqueux fournissant chacun une distribution de  radicaux libres (e-aq, H,OH, H+aq, H2, H2O2, OH-, O2, O-2, OH2, OH2- que nous avons fait évoluer selon un ensemble de codes informatiques élaborés sur un modèle déterministe jusqu’à leur collision et estimer ainsi la concentration et le rendement de chaque espèce. Dans cette étude, nous n’avons considéré queles espèces les plus agressives vis à vis des molécules biologiques e-aq, H et plus particulièrement l’hydroxyle OH. Nous avons ainsi estimé le nombre moyensde diverses lésions portées à l’ADN. L’intérêt essentiel de cette étude est lamise au point d’un modèle simple d’adaptation facile du point de vue applications qui peut renseignersur les dommages causés à une molécule aussi importante que l’ADN.

  9. Structure par RMN d'un complexe AlcR(1-60)-ADN: Reconnaissance du petit sillon par la partie N-terminale

    Science.gov (United States)

    Cahuzac, B.; Félenbok, B.; Guittet, E.

    1999-10-01

    Aspergillus nidulans is a filamentous fungus able to use ethanol as sole energy source. The activation of the ethanol regulon genes expression is mediated by the AlcR protein. Its DNA-binding domain is located in the N-terminus (residues 1 to 60), and its NMR solution structure shows a global zinc binuclear cluster fold, with two helices in addition to the basic binuclear motif. A small number of crystallographic structures of DNA complexes of binuclear cluster proteins is yet known, and points out the major groove and the first helix as the principal sites of interaction on the DNA and the protein respectively. In this article we show evidences that the N-terminus of the protein is involved in binding to the minor groove. Aspergillus nidulans est un champignon filamenteux capable d'utiliser l'éthanol comme source unique d'énergie. La protéine AlcR est responsable de l'activation de l'expression des gènes du régulon éthanol. Le domaine de liaison à l'ADN est situé dans la partie N-terminale de la protéine (a.a. 1 à 60), et sa structure déterminée par RMN en solution montre un repliement global en bouquet binucléaire à zinc, avec deux hélices supplémentaires par rapport au motif de base. Alors que les structures déjà connues de complexes ADN - bouquets binucléaires permettent de situer dans le grand sillon la quasi-totalité des interactions, nous montrons dans la présente étude l'implication du début de la séquence dans la reconnaissance du petit sillon de l'ADN (a.a. 5 et 6).

  10. Combustion Properties of PGN/ADN Propellants%PGN/ADN推进剂的燃烧性能

    Institute of Scientific and Technical Information of China (English)

    尚冬琴; 黄洪勇

    2010-01-01

    采用靶线法在3.0~15.0 MPa压强范围内,研究了固含量、铝粉含量、二硝酰胺铵(ADN)的粒径及含量、燃速调节剂及热稳定剂对PGN/ADN推进剂燃烧性能的影响.结果表明,ADN的粒径(<450 μm,450~900 μm)和含量(0%~30%)增加时,PGN/ADN推进剂的燃速和压强指数均适当增加; 改变Al粉含量,PGN/ADN推进剂的燃速和压强指数均无明显变化; 添加适量(0.5%)燃速调节剂Fe2O3可增加推进剂的燃速并降低压强指数,添加适量(0.5%)燃速调节剂草酰胺可有效降低压强指数. 添加适量稳定剂(1%)2-硝基二苯胺(2-DNPA)和N-甲基-4-硝基苯胺(MNA)可以使推进剂的压强指数分别由0.49降低到0.34和0.40.

  11. Papel de los polimorfismos genéticos para enzimas de reparación en el daño en el ADN inducido por el estireno y estireno-7,8-óxido

    Directory of Open Access Journals (Sweden)

    B. Laffon

    2004-01-01

    Full Text Available La respuesta individual al daño en el ADN inducido por agentes xenobióticos está condicionada por la eficacia de los sistemas de reparación. Algunos de los polimorfismos genéticos descritos en las enzimas de reparación del ADN pueden afectar a su función, determinando una variación en la susceptibilidad ante la exposición a agentes ambientales. El objetivo de este estudio ha consistido en investigar si las variantes alélicas más frecuentes de las enzimas de reparación XRCC1 (Arg194Trp y Arg399Gln, XRCC3 (Thr241Met o APE1 (Asp148Glu pueden condicionar el daño en el ADN inducido por el estireno y su principal metabolito, el estireno-7,8-óxido (EO. Leucocitos periféricos de 30 voluntarios sanos se trataron con estireno o EO, y el daño en el ADN inducido se evaluó mediante el ensayo del cometa. Tras el tratamiento con estireno, los individuos portadores de los alelos XRCC1 399Gln y XRCC3 241Met mostraron mayor nivel de roturas en el ADN, sugiriendo menor eficacia de los sistemas de reparación. Por el contrario, los portadores del alelo APE1 148Glu mostraron daño en el ADN significativamente menor que los individuos 148 Asp/Asp. Sin embargo, no se obtuvo ningún efecto significativo en las células expuestas a EO, debido probablemente a que el daño inducido es inicialmente mayor, y no permite que se pongan de manifiesto pequeñas diferencias en la eficacia de reparación de los genotipos analizados

  12. Valoración de la prueba de ADN en el proceso penal: cuestiones científicas, legales y de probabilidad

    OpenAIRE

    González Ortega, Adela María

    2016-01-01

    El principal objetivo de este trabajo es tratar de explicar todas las etapas que conciernen a la prueba de ADN aplicada a casos de criminología, así como todos los problemas que se plantean por la utilización de esta prueba en los juicios penales. Algunos de los puntos clave que se pretenden abordar en este trabajo son: analizar la información que se obtiene de esta prueba en el laboratorio de Biología Forense y el valor que se le da en la actualidad en la resolución de un juicio penal. D...

  13. Impacto de la fragmentación del ADN espermático en la población infértil

    OpenAIRE

    Alvarez de la Fuente, Myrian

    2013-01-01

    La fragmentación del ADN espermático está empezando a considerarse como un nuevo parámetro de calidad seminal. Actualmente, existe mucha controversia en torno a la importancia de su estudio así como la influencia que puede tener en las tasas de fecundación, implantación, embarazo, aborto y calidad embrionaria. El objetivo del trabajo es evaluar si existe una relación entre alguno de los parámetros de un seminograma básico y el grado de fragmentación que presentan nuestra población en est...

  14. 二硝酰胺铵(ADN)的燃烧特性研究进展%Combustion characteristics and mechanisms of ammonium dinitramide

    Institute of Scientific and Technical Information of China (English)

    杨荣杰

    2003-01-01

    综述了国外二硝酰胺铵(ADN)燃烧特性和燃烧机理研究的进展,包括ADN及其复合推进剂的燃烧特点,燃速-压力依赖关系,燃烧波温度分布特征,初始分解机理,低压下燃烧暗区形成的机理和存在燃速不稳定压力区的原因分析.此外,对暗区形成的机理进行了讨论.

  15. Optimización de un protocolo de extracción de ADN genómico para Pinus tecunumanii

    OpenAIRE

    2013-01-01

    Se optimizó un protocolo para la extracción de ADN genómico de Pinus tecunumanii basado en el método de extracción de Doyle y Doyle (1990). Se probaron los megagametofitos de las semillas de árboles deP. tecunumanii muestreados en cinco poblaciones naturales de Nicaragua. El método consta de maceración del tejido en tubos Eppendorf, una extracción con bromuro trimetil amonio de cetilo (CTAB)empleando altas concentraciones de sales, proteinasa K, extracciones sucesivas con cloroformo-alcohol i...

  16. Evaluación de cuatro métodos de extracción del ADN de Histoplasmacapsulatum y su uso en reacciones de PCR

    Directory of Open Access Journals (Sweden)

    Ernesto Xavier Monroy-Vaca

    2014-08-01

    Full Text Available Histoplasmacapsulatum es un hongo endémico de Cuba y el agente causal de la histoplasmosis. Esta micosis se presenta en forma de brotes epidémicos, afecta a las personas, independiente de su estado inmunológico. Su diagnóstico se realiza mediante técnicas microbiológicas, serológicas e histológicas que tienen una sensibilidad moderada y son demoradas. Por lo que se hace necesario la estandarización de métodos moleculares rápidos y eficientes para el diagnóstico de esta micosis. Se evaluaron cuatro métodos de extracción del ADN (enzimático, mecánico y dos químicos: tiocianato de guanidinio y Tritón X-100 a partir de una cepa de H. capsulatum en fase filamentosa. Se determinó la concentración, la pureza, el rendimiento y la integridad del ADN mediante espectrofotometría y electroforesis en gel de agarosa. Se evaluaron dos técnicas de PCR con el ADN obtenido, con cebadores específicos de especie y otro con un cebador arbitrario. Los mejores resultados de concentración, pureza, rendimiento e integridad se obtuvieron con el método de extracción enzimático. Se logró la estandarización de las PCR con cebadores específicos, confirmándose así las 16 cepas estudiadas como H. capsulatum. Con el cebador arbitrario se amplificaron secuencias diferentes en las cepas ensayadas. Se demostró que el método de extracción enzimática brindó un ADN de buena calidad para su utilización en PCR. Este trabajo facilita el diagnóstico rápido y confiable de H. capsulatum en Cuba y brinda la posibilidad de identificar cepas con estructuras relacionadas genéticamente con los marcadores de patogenicidad y la virulencia, útiles para conformar un inmunógenovacunal.

  17. Evaluación de métodos de extracción de ADN para detección de Listeria monocytogenes en productos cárnicos

    Directory of Open Access Journals (Sweden)

    Lina López DA.

    2012-12-01

    Full Text Available Objetivo. Evaluar dos procedimientos para la extracción de ADN de Listeria monocytogenes a partir de muestras de alimentos contaminados artificialmente. Materiales y métodos. Se evaluaron diferentes métodos de extracción en cultivos puros de L. monocytogenes. Los métodos con mejores resultados fueron evaluados en muestras de alimentos cárnicos (jamón, salchicha y chorizo contaminados artificialmente. Para evaluar la calidad del ADN extraído se determinó la concentración de ADN, la relación A260/A280 y se amplificó el gen hlyA de L. monocytogenes por PCR. Resultados. Los métodos con solventes orgánicos y con PBS + Tween 20 permitieron obtener mayor cantidad de ADN (40 y 50 μg, respectivamente. En muestras de alimentos, se obtuvo ADN de mayor pureza con el método con solventes orgánicos (p <0,005, pero con el método con PBS + Tween 20 se obtuvo una mayor concentración. Con ambos métodos de extracción de ADN se logró la amplificación del gen hlyA en muestras contaminadas desde 1 hasta 105 UFC/ml. La composición del alimento no afectó la reacción de PCR en las muestras de ADN obtenidas con los dos métodos de extracción. Conclusiones. Independientemente del método de extracción utilizado, se logró la detección del gen hlyA de L. monocytogenes en muestras de alimentos contaminados desde 1 hasta 105 UFC /ml. Sin embargo, para su uso como método rutinario de diagnóstico, el método con PBS + Tween 20 es la mejor opción para la extracción de ADN, por ser un método de fácil aplicación, bajo costo y buen desempeño.

  18. Análisis de la fragmentación del ADN de espermatozoides humanos mediante el test de dispersión de la cromatina espermática : origen, causas e impacto en reproducción asistida

    OpenAIRE

    Santiso Brandariz, Rebeca

    2013-01-01

    [Resumen] Los hombres infértiles presentan una mayor proporción de espermatozoides con ADN fragmentado que los hombres fértiles, aunque un cierto porcentaje de estas células siempre está presente en todas las muestras seminales. Esta tesis se centra en el estudio de ciertas cuestiones básicas y clínicas acerca de la fragmentación del ADN espermático. Desde el punto de vista de la investigación básica, nuestros resultados indican que el estrés oxidativo se relaciona con la fragmentación del AD...

  19. Comparación de dos métodos de extracción de ADN a partir de plantas del género Solanum, subgénero Leptostemonum.

    Directory of Open Access Journals (Sweden)

    Isabel Cristina Cadavid Sánchez

    2013-12-01

    Full Text Available Título en ingles: Comparison of two ADN extraction methods from plants belonging Solanum genus Leptostemonumsubgenus.Título corto: Extracción de ADN de SolanumResumen: Se evaluaron dos métodos para la extracción de ADN en plantas del género Solanum, con el fin de obtener ADN disponible y de buena calidad para la obtención de secuencias. El producto comercial DNeasy® Plant Mini Kit se comparó con un método que incluye el uso de una solución tampón de lisis. Para este último método también se evaluó si el rendimiento mejoraba cuando las muestras se maceraron previamente con  nitrógeno líquido. Los resultados en términos de calidad (A260/A280 no mostraron diferencias significativas entre los métodos de extracción (índice < 1,5. Sin embargo, se encontraron diferencias en la concentración de ADN obtenida (prueba de Dunnet, p<0,05 y en los porcentajes de amplificación mediante PCR (X2, p<0,05. Los mejores resultados, en cuanto al éxito en la PCR (89%, se obtuvieron con el producto comercial, sin embargo la dilución 1:100 de las muestras obtenidas con el método de solución de lisis, permitió obtener resultados de PCR comparables. La maceración de las muestras con nitrógeno líquido, también mejoró el rendimiento (éxito de PCR del método con solución de lisis. Se propone este método  como una alternativa costo-efectiva para la extracción de ADN a partir de plantas del género evaluado, con base en los resultados obtenidos.Palabras clave: extracción, ADN, planta, solución tampón, PCR.Abstract: Two methods were evaluated for ADN extraction in plants belonging to the Solanum genus. The objective was to isolate adequate ADN amount and of enough quality for obtaining sequences. The commercial product DNeasy® Plant Mini Kit was compared with a method that includes a lysis buffer. For the last method it was also evaluated the improvement   when samples were previously macerated with liquid nitrogen. The quality

  20. ADN及其混合物燃烧性能的理论计算%THEORETIC CALCULATION OF THE BURNING RATE CHARACTERISTICS OF ADN AND ITS MIXTRUES

    Institute of Scientific and Technical Information of China (English)

    杨栋; 宋洪昌; 赵凤起; 李上文

    1998-01-01

    分析了新型含能材料二硝酰胺铵(ADN)的热分解特性和燃烧特性,提出ADN燃烧初期热分解反应的假说,建立了由化学结构特征计算ADN及其混合物燃速公式,并计算了ADN和配比不同的ADN混合物的燃速特征.计算结果与实测值十分一致.从化学结构和化学反应的层次分析了ADN单质燃烧出现平台燃烧现象的原因,认为ADN热分解过程中,中间产物NH4NO3的形成和消失在平台(麦撒)燃烧中起着关键作用.预测了ADN提高固体推进剂燃速和降低燃速压力指数的趋势.

  1. Protocolo de extracción de ADN en lotes de 10 mosquitos para la identificación de Plasmodium spp. mediante qPCR

    Directory of Open Access Journals (Sweden)

    A. Pérez Rico

    Full Text Available Las tropas que despliegan en zonas de operaciones endémicas de malaria, necesitan de una información precisa del riesgo sanitario para la toma de decisiones acerca de las medidas de prevención más adecuadas. El estado de portador de un mosquito se determina clásicamente por la presencia o ausencia de esporozoitos de Plasmodium spp. en las glándulas salivales. Los protocolos basados en la amplificación del ADN en tiempo real (qPCR son muy sensibles, sin embargo existen dificultades en la qPCR debido a inhibidores presentes en los tejidos del mosquito, lo que obliga a trabajar de uno en uno. En este trabajo se diseña una qPCR para amplificar una región conservada entre mosquitos de diferentes especies y otros dípteros, con el objetivo de comparar varios protocolos de extracción de ADN y determinar el más eficiente a la hora de procesar lotes de 10 mosquitos.

  2. Revisión sobre la genética del origen del ganado vacuno y las aportaciones del ADN antiguo

    Directory of Open Access Journals (Sweden)

    Lira, J.

    2010-01-01

    Full Text Available Actualmente el ganado vacuno es la especie doméstica económicamente más importante de todas las que explota el hombre. Por este motivo este trabajo hace un repaso de los estudios que se han llevado a cabo sobre la aparación del ganado doméstico, teniendo en cuenta las evidencias aruqeológicas y genéticas. Durante las dos últimas décadas el análisis del ADN mitocondrial se ha mostrado como una herramienta muy útil para profundizar en los focos de origen, migración y posibles eventos independientes de domesticación, tanto del ganado taurino como del ganado cebú. Se ofrece una revisión de los estudios que se han centrado en el desarrollo de los linajes maternos del ganado doméstico, analizando ADN mitocondrial de individuos modernos y de restos antiguos de diferentes cronologías. Además, exponemos los resultados complementarios obtenidos a partir del análisis del cromosoma Y extraído de muestras antiguas.

  3. Divergencia intraespecífica y código de barras de ADN en Systrophia helicycloides (Gastropoda, Scolodontidae

    Directory of Open Access Journals (Sweden)

    Pedro Romero

    2011-08-01

    Full Text Available El DNA barcoding es un análisis que se basa en la comparación de distancias genéticas para identificar especies utilizando principalmente un segmento del gen Citocromo C Oxidasa I (COI. Los retos para la identificación surgen al estudiar grupos que presentan gran diversidad genética como los moluscos. Por ello, los objetivos de nuestra investigación fueron estimar la divergencia intraespecífica en el molusco terrestre amazónico Systrophia helicycloides (Gastropoda, Scolodontidae y evaluar la utilización de los códigos de barras de ADN en la identificación molecular de esta especie. Las secuencias de nucleótidos fueron comparadas con las bases de datos Genbank y BOLD (Barcode of Life Data Systems. Se realizó un análisis de distancia genética mediante Neighbour Joining. Systrophia helicycloides presentó dos grupos de haplotipos con distancias genéticas intraespecíficas mayores a 4%. También se observó una superposición entre las distancias intraespecíficas y las interespecíficas. La gran divergencia intraespecífica puede estar relacionada a la rápida variación del genoma mitocondrial, la distribución poblacional de los moluscos la cual permite el aislamiento y diferenciación genética, y la presencia de polimorfismos ancestrales. Los perfiles COI enviados a la base de datos BOLD son los primeros registros para esta especie y permitieron diferenciar a Systrophia helicycloides de otras especies. Estos perfiles corroboran la gran variación que ocurre en el genoma mitocondrial de moluscos terrestres por lo que la asignación de especies en este grupo precisa de la combinación entre los valores de divergencia genética, la evaluación de sitios informativos y los estudios de taxonomía convencional.

  4. EFECTO IN VITRO DE LA UNCARIA TOMENTOSA, DEL MTA Y DEL HIDROXIDO DE CALCIO EN LA VIABILIDAD CELULAR, MORFOLOGIA NUCLEAR Y INTEGRIDAD DEL ADN EN PULPA DENTAL DE PIEZAS DENTARIAS EXTRAIDAS, CLINICA ODONTOLOGICA PARTICULAR. AREQUIPA, 2011

    OpenAIRE

    LÓPEZ HUAMÁN, GIOVANNI

    2013-01-01

    UNCARIA TOMENTOSA ANTECEDENTES HISTÓRICOS CLASIFICACIÓN BOTÁNICA COMPOSICIÓN QUÍMICA PROPIEDADES FARMACOLÓGICAS TOXICIDAD Y CONTRAINDICACIONES EFECTOS MTA HIDRÓXIDO DE CALCIO COMPLEJO DENTINO PULPAR RECUBRIMIENTO PULPAR DIRECTO VIABILIDAD CELULAR MORFOLOGÍA NUCLEAR ESTRUCTURA DEL ADN

  5. Detección del estado de metilación de los genes dapk, cdh13, cdh1 y rassf1 en ADN de plasma de pacientes con cáncer de cuello uterino

    Directory of Open Access Journals (Sweden)

    Aristizábal Fabio

    2005-07-01

    Full Text Available El estudio de las características epigenéticas en ADN proveniente de plasma de pacientes con cáncer de cuello uterino (CCU tiene un futuro promisorio;  se han encontrado previamente genes supresores de tumor (GST metilados, correlacionados con estadios avanzados del CCU, siendo
    posibles indicadores de peor pronóstico y marcadores moleculares de respuesta a tratamiento. Sin embargo, no existe ningún estudio para Colombia, en el que se haya buscado detectar estados de metilación para ADN de plasma en ningún tipo de cáncer. En este trabajo se reporta el estudio
    de 23 pacientes colombianas con estadios avanzados (III y IV de CCU (Banco de Muestras del Instituto Nacional de Cancerología, a los cuales les fue detectado el estado de mutilación (conversión por bisulfito de sodio posterior MSP de los GST dapk, cdh13, cdh1 y rassf1, en ADN de plasma,
    y se comparó contra el estado de metilación en ADN de plasma, arrojando los siguientes porcentajes de pacientes que presentaron el mismo estado de metilación (presente/ausente rassf1, 44%; cdh13, 33%; cdh1, 44%; dapk, 78%; para un total de los cuatro genes en conjunto de 47%. Adicionalmente, se detectó la presencia en el 100% de las muestras de tumor de HPV tipo 16. Se demostró igualdad entre las poblaciones de tumor y plasma para el panel de los cuatro genes (p=0,635, Test de McNemar a=0,05, en particular para el estadio III (p=0,85. El gen dapk presentó un estado de metilación positivo para plasma del 68,4% y para tumor del 94% en estadios avanzados. De esta manera, se consiguió la detección de los estados de metilación en ADN de plasma y se encontró correlación estadística con los encontrados en ADN tumoral, en particular para el estadio III. Este trabajo constituye un aporte importante para el uso de características epigenéticas de ADN de plasma, como marcadores moleculares de progresión, respuesta a tratamiento, y suprevivencia, en pacientes colombianas con CCU.

  6. Complexation des acides aminés basiques arginine, histidine et lysine avec l'ADN plasmidique en solution aqueuse : participation à la capture de radicaux sous irradiation X à 1,5 keV

    Science.gov (United States)

    Tariq Khalil, Talat; Taillefumier, Baptiste; Boulanouar, Omar; Mavon, Christophe; Fromm, Michel

    2016-09-01

    L'environnement chimique de l'ADN en situation biologique est complexe notam-ment en raison de la présence d'histones, protéines nucléaires, associées en quantité approximativement égales à l'ADN pour former la chromatine. Les histones possèdent de nombreux radicaux basiques arginine et lysine chargés positivement et dont la majorité se trouve sur les chaînes émergentes, l'ADN présente quant à lui des charges négatives sur ses groupements phosphates localisés tout au long de la double hélice. Dans cette étude, la complexité de la structure de la chromatine nucléaire est dans un premier temps mimée en solution aqueuse par la formation de complexes entre un ADN plasmidique sonde et les trois acides aminés basiques, Arg, His, Lys, qui, mis à part His, sont protonés au pH physiologique. Ces acides aminés libres en solution sont réputés être des capteurs efficaces de radicaux libres, notamment pour le radical hydroxyle, conférant ainsi un pouvoir protecteur vis-à-vis des effets indirects sur l'ADN en situation d'exposition aux rayonnements ionisants. A concentration fixée, les capacités de capture des acides aminés libres, σ, pour le radical hydroxyle sont typiquement les suivantes σHis ≈σArg > σLys (σLys ≈ 0,1 × σArg). Nous avons mesuré les taux de cassures simple brin par plasmide et par Gray (χ) lors d'expositions de solutions aqueuses de complexes [acide aminé - ADN plasmidique] aux rayons X ultra-mous (1,5 keV). A concentrations égales, les trois acides aminés complexés et présents en large excès ne manifestent pas une capacité de protection de l'ADN proportionnelle à leur capacité de capture libre et en solution ; on trouve en effet des taux de cassures dans l'ordre suivant χHis > χArg > χLys (χLys ≈ 0,01 χArg). Après avoir détaillé le mode opératoire de ces mesures, nous analyserons sur des bases bibliographiques, les modes spécifiques d'interaction des acides aminés basiques avec l'ADN. La sp

  7. Daño inducido en el ADN plasmidial por la radiación mixta de neutrones térmicos + rayos gamma en presencia y ausencia de capturador de radicales libres

    Directory of Open Access Journals (Sweden)

    Maritza Rodríguez-Gual

    2010-01-01

    Full Text Available En este trabajo se cuantifica por primera vez el daño en ADN plasmidial inducido por la radiación mixta de neutrones térmicos + rayos gamma. Para el estudio fue utilizado el plasmidio pBs KS+ de 2 961 pb en disolución acuosa a 88 ng/¿L a concentraciones de 0, 2 y 20 mmol/L de glicerina que actúa como un capturador de radicales libres. Este plasmidio cambia su forma superenrollada para circular cuando es producida una rotura simple en su cadena y adquiere una forma linear cuando se produce una rotura doble en su cadena. Cuantificando las fracciones que se producen de cada una de estas formas es posible estimar el efecto de la radiación en el ADN. Las irradiaciones fueron realizadas en el canal radial # 3 del reactor de investigaciones IEA-R1 del Instituto de Pesquisas Energéticas y Nucleares de São Paulo, Brasil. Las formas del ADN fueron separadas mediante la electroforesis en gel de agarosa. En la cuantificación se utilizó el programa GelAnalis. Las fracciones de ADN en sus diferentes formas fueron graficadas en función de la dosis y ajustadas a funciones exponenciales y lineares para obtener las probabilidades de roturas simples y dobles en la cadena de ADN normalizadas por dosis y por masa molecular. Los resultados evidenciaron la acción protectora del capturador de radicales libres contra el daño inducido por la radiación, lo cual corrobora resultados anteriores obtenidos con otras radiaciones ionizantes. Los rendimientos de SSB y DSB serán de interés para la validación de diferentes modelos que intentan reproducir los resultados experimentales.

  8. Detección “in vivo” mediante RAPD de alteraciones en el ADN producidas por benzo(apireno

    Directory of Open Access Journals (Sweden)

    C Becerril

    2004-01-01

    Full Text Available La técnica de RAPD (Amplificación al Azar de ADN Polimórfico permite detectar alteraciones inespecíficas en el ADN procedente de células que poseen una dotación genética idéntica, como son las líneas celulares establecidas, mediante la comparación del patrón de bandas de las células expuestas y no expuestas a la acción de genotóxicos. En los últimos años hemos desarrollado una metodología sensible y reproducible utilizando la línea celular RTG-2, derivada de trucha arco iris (Oncorhynchus mykiss. Sin embargo, es preciso comprobar la capacidad predictiva de este ensayo mediante estudios in vivo. La línea celular RTG-2, como se ha evidenciado en trabajos anteriores, presenta una gran similitud genética con la especie de la que procede. Por ello, en este trabajo, se ha llevado a cabo una exposición subletal a benzo(apireno mediante inyección intraperitoneal de 69 μg/g de p.c. en alevines de trucha arco iris, valorando la aparición de mutaciones mediante la comparación del patrón de bandas obtenido a partir del ADN de células de sangre periférica, a diferentes tiempos (1 - 3 meses. Debido a que la presencia de bandas polimórficas dificulta el análisis entre los grupos de individuos tratados y no tratados, las comparaciones se realizaron en un mismo individuo antes y después del tratamiento. Los análisis cualitativos y cuantitativos mostraron tanto la aparición de nuevas bandas, como alteraciones en su intensidad confirmando, de esta manera, los resultados que previamente habíamos obtenido in vitro tras exposiciones a este mismo genotóxico

  9. Study on Interaction of ADN and (NC+NG) by Gasometric Method%量气法研究ADN与(NC+NG)的相互作用

    Institute of Scientific and Technical Information of China (English)

    何少蓉; 张林军; 衡淑云; 刘子如

    2008-01-01

    用 NBK型"拉瓦"量气测试系统研究了较高温度下二硝酰胺铵(ADN)与双基吸收药(NC+NG)体系的相互作用,从动力学角度阐述了二者的相互作用.结果表明: ADN与(NC+NG)混合物加热分解的最终放气量在标准状态下为626.8 mL·g-1,与ADN和(NC+NG)最大放气量之和相比并没有增加,但分解速度显著提高,活化能显著降低至82.58 kJ·mol-1,ADN与(NC+NG)之间存在强烈的相互作用.

  10. Dinámica de la molécula más importante de la vida: el ADN. I. Modelos lineales y no lineales

    Directory of Open Access Journals (Sweden)

    Lorena Ramírez Reyes

    2005-01-01

    Full Text Available En este artículo se revisan los modelos más simples para la molécula más importante dela vida: el ADN. Se estudian las excitaciones colectivas y paulatinamente van incorporándose en elanálisis cualidades complejas de la molécula. Esto conlleva al estudio de la dinámica de talesexcitaciones mediante ecuaciones diferenciales no lineales. Las soluciones especiales de estasecuaciones corresponden a un tipo de ondas no lineales que mantienen forma y velocidadconstantes con la mínima pérdida de energía e información, conocidas como solitones ocompactones.

  11. Uso del ADN forense en la Policía Nacional para identificación de alias “Édgar Tovar”

    Directory of Open Access Journals (Sweden)

    Martha Liliana Acevedo Neira

    2010-07-01

    Full Text Available El análisis genético de fragmentos óseos tomados de un cuerpo en descomposición permitió confirmar la muerte de alias “Édgar Tovar”, comandante y responsable del narcotráfico del frente 48 de las Farc. El perfilgenético obtenido de los restos humanos fue comparado con la información del ADN nuclear y de cromosoma Y, del padre de Ángel Gabriel Lozada García,obteniendo una probabilidad estadística de paternidad de 99.991% y una coincidencia total en la información contenida en los marcadores de cromosomaY analizados, lo que sumado a labores investigativas de la Policía Nacional se consideró suficiente para determinar la identificación del cuerpo.

  12. IMPLEMENTACIÓN DE LA METODOLOGÍA DE ANÁLISIS DE ADN MITOCONDRIAL EN Rhinoclemmys nasuta (TESTUDINES:GEOEMYDIDAE

    Directory of Open Access Journals (Sweden)

    YHERSON FRANCHESCO MOLINA HENAO

    2014-01-01

    Full Text Available Rhinoclemmys nasuta (Testudines: Geoemydidae es considerada una especie casi endémica de Colombia y la más primitiva del género, sin embargo, se encuentra clasificada por la IUCN como deficiente de datos, ya que la información disponible no es suficiente para hacer una evaluación directa o indirecta de su riesgo de extinción. En este trabajo se describe la implementación del método para realizar la secuenciación de la región control del ADN mitocondrial de R. nasuta, con el propósito de generar herramientas técnicas para futuros estudios de evolutivos y de conservación. Se utilizó el método de desalamiento (Salting-out para extraer ADN a muestras sanguíneas procedentes de Isla Palma y Playa Chucheros (Bahía Málaga–Pacífico Colombiano y se utilizó una pareja de cebadores degenerados (reportada para Chrysemys picta Testudines: Emydidae para realizar la amplificación. Se obtuvieron fragmentos de 800pb siendo exitosa la reacción de secuenciación de los amplificados, y se estableció un porcentaje de homología mayor al 92 % entre las secuencias obtenidas y las secuencias de ADNmt de Sacalia quadriocellata (Testudines:Geoemydidae y Cuora aurocapitata (Testudines:Geoemydidae, depositadas en el GeneBank. Este resultado demuestra que el método descrito puede ser una herramienta útil para el estudio de las poblaciones de R. nasuta del Pacífico colombiano, lográndose una efectiva secuenciación de la región control del ADNmt de esta especie.

  13. Historias en código genético: Los aportes de los estudios de ADN antiguo en antropología y sus implicancias éticas Historias em código genético: As contribuições dos estudos de ADN antigo em antropologia e suas implicâncias éticas Stories in Genetic Code: The contribution of ancient DNA studies to anthropology and their ethical implications

    Directory of Open Access Journals (Sweden)

    Cristian M. Crespo

    2010-12-01

    Full Text Available Desde hace varias décadas, la antropología biológica comenzó a utilizar marcadores moleculares en estudios poblacionales. A partir de la década de 1990 se desarrollaron diferentes técnicas en biología molecular que permitieron extraer y tipificar ADN conservado en diferentes restos provenientes de museos y sitios arqueológicos. Los estudios de ADN antiguo relacionados con problemáticas arqueológicas conforman hoy un campo de estudio denominado Arqueogenética. Se presentan en este trabajo algunas de las aplicaciones del ADN antiguo. Se discuten las ventajas y limitaciones de estos estudios y su relación con temas éticos y legales.Faz já varias décadas que à antropologia biológica tem começado a usar marcadores moleculares em estudos de população. Ao começo da década de 1990, se desenvolveram técnicas diferentes em biologia molecular que permitiram obter e tipificar o ADN conservado em restos diferentes vindos de museus e sítios arqueológicos. Os estudos de ADN antigo relacionados a problemáticas arqueológicas são hoje um campo de estudo chamado de Arqueogenética. Se apresentam neste trabalho algumas das aplicações do ADN antigo. Também se discutem as vantagens e limitações desses estudos e a sua relação com assuntos éticos e legais.For several decades, biological anthropology has employed different molecular markers in population research. Since 1990 different techniques in molecular biology have been developed allowing preserved DNA extraction and its typification in different samples from museums and archaeological sites. Ancient DNA studies related to archaeological issues are now included in the field of Archaeogenetics. In this work we present some of ancient DNA applications in archaeology. We also discuss advantages and limitations for this kind of research and its relationship with ethic and legal norms.

  14. 1.54 μm electroluminescence from ErQ doped ADN organic light-emitting diodes%有机发光二极管中ADN掺杂ErQ的1.54μm电致发光

    Institute of Scientific and Technical Information of China (English)

    屈海京; 陶利; 王维; 冉广照

    2014-01-01

    自行设计了基于8-羟基喹啉铒(ErQ)为发射层(EMLs)和二硝酰胺铵(ADN)为蓝光主体材料的近红外有机发光二级管.器件的基本结构为(p-Si/NPB/EML/Bphen/Bphen∶ Cs2CO3/Sm/Au),设计并比较了三套不同发射层结构(ErQ/ADN为双层结构器件,(ErQ/ADN)×3为多层结构器件,ErQ∶ ADN为掺杂结构器件)的器件.三组器件在一定的偏压下,均可发出1.54 μm的光,对应三价铒离子4I13/2→4I15/2的跃迁.其中,ADN∶ErQ(1∶1)掺杂结构的近红外电致发光强度是ADN/ErQ双层结构中的三倍.此外,不同掺杂浓度的ADN:ErQ复合膜做了以下表征:吸收谱、光致发光谱和荧光寿命谱.实验结果证实了在近红外电致发光过程中存在从ADN主体分子到ErQ发射分子的高效率的能量转移.

  15. Método de amplificación de ADN basado en los orígenes de replicación del bacteriófago Phi29 y secuencias nucleotídicas asociadas

    OpenAIRE

    2011-01-01

    La presente invención se refiere a un método de amplificación de ADN basado en los orígenes de replicación del bacteriófago φ29, así como a las construcciones génicas, vectores y oligonucleótidos que pueden emplearse en dicho método para amplificar una secuencia exógena de interés.

  16. 二硝酰胺铵的致突变性和致畸性研究%Study on mutagenicity and teratogenicity of ammonium dinitramide (ADN)

    Institute of Scientific and Technical Information of China (English)

    杜文霞; 王玉玲; 孙苑菡; 谢锋; 常志强; 邢亚飞; 张延巍

    2011-01-01

    Objective To study the mutagenicity and teratogenicity induced by ammonium dinitramide (ADN).Methods According to technical specifications for toxicity determination of chemicals,Salmonella typhimurium reverse mutation assay (Ames assay),in vivo mammalian erythrocyte micronucleus test,sperm malformation test and teratogenesis test were used to detect the mutagenicity and teratogenicity induced by AND.Results When the exposure doses of AND were 8~5000 μg/plate,the result of Ames assay was negative.As compared with control group,the micronucleus rate of mice exposed to 113.8 mg/kg AND significantly increased (P<0.05),the sperm malformation rates of mice exposed to 54.4~272.0 mg/kg AND did not increased significantly.The survival rate of fetuses decreased,the rate of assimilated fetuses increased,the rate of fetus sternum agenesis enhanced in mice exposed to 319 mg/kg AND,as compared with controls.The rates of in the 4th-6th fetus sternum agenesis in groups exposed to 21.3,79.7 and 319 mg/kg AND were higher than that in control group.The malformation rate of fetus bowels in groups exposed to 319 mg/kg AND was higher than that in control group.The teratogenic index of ADN was 30.Conclusion AND may be a mutagen and induce the teratogenic effect.%目的 对新型含能材料二硝酰胺铵(ADN)的致突变性和致畸性进行研究,为进一步研究ADN对人体健康影响和制订职业接触限值提供依据.方法 根据《化学品毒性鉴定技术规范》,采用鼠伤寒沙门杆菌回复突变试验(Ames试验)、体内哺乳动物骨髓嗜多染红细胞微核试验、精子畸形试验和致畸试验研究ADN的致突变性和致畸性.结果 (1)ADN在8~5000 μg/皿剂量范围内未发现诱变作用;113.8mg/kg剂量染毒组与阴性对照相比,诱发小鼠的微核率明显升高,差异有统计学意义(P<0.05);54.4~272.0 mg/kg剂量范围内小鼠精子畸形试验结果为阴性.(2)319 mg/kg剂量组活胎率低于阴性对照组,吸

  17. Semejanzas entre el ajo (Allium sativum costarricense y el ajo asiático según secuencias de ADN ribosomal

    Directory of Open Access Journals (Sweden)

    Karina Barboza Rojas

    2012-08-01

    Full Text Available En Costa Rica, la producción de ajo (Allium sativum es escasa y se limita principalmente a las regiones de Llano Grande de Cartago. Sin embargo, por el precio que tiene actualmente en el mercado, el ajo costarricense se vislumbra como una hortaliza que podría constituirse en un recurso valioso para los productores nacionales. Por lo anterior, en este artículo se presenta la caracterización mediante secuencias ribosomales de materiales de ajo provenientes Costa Rica y su comparación con ajo importado de China. El ADN se extrajo a partir de hojas de vitroplantas de ajo mantenidas en un congelador a -70°C, pulverizadas en un mortero. El ADN ribosomal se amplificó, purificó y secuenció. Se realizó el análisis bioinformático de las secuencias ribosomales. El BLASTn permitió determinar que los productos de PCR amplificados corresponden a la secuencia parcial de los genes 28S y 18S (sitios de unión de los cebadores y a la secuencia completa de la región ITS-1, 5.8S e ITS-2. Se encontró que todas las secuencias alinearon en casi un 100% con la accesión EU626375.1 publicada en la base de datos del GeneBank, correspondiente al clon Allium sativum voucher BF-ALL-037. En general, las secuencias mostraron ser muy conservadas. Los puntajes obtenidos del alineamiento realizado con ClustalW reflejaron una identidad del 97 al 99% entre las secuencias.El presente estudio es el primer reporte de este tipo que se realiza sobre ajo costarricense y generó información básica e indispensable para continuar con los estudios moleculares de este cultivo.

  18. La fotografía en los diarios impresos gratuitos españoles: aplicación y uso en 20 Minutos, Qué y Adn Photography in the daily Spanish free press: Application and use in 20 Minutos, Qué and Adn

    Directory of Open Access Journals (Sweden)

    Juan Miguel Sánchez Vigil

    2012-12-01

    Full Text Available La prensa gratuita, de gran impacto social, cumple una función informativa de urgencia con titulares de impacto, artículos concisos y noticias breves. La distribución en puntos clave (medios de transporte, cafés, centros culturales, bibliotecas, etcétera. confiere a este medio unas características especiales poco estudiadas, entre ellas la fotografía. Se presenta en este artículo un estudio exhaustivo de la aplicación y uso de la fotografía en los tres diarios impresos gratuitos españoles de implantación nacional: 20 Minutos, Qué y Adn, considerando el número de imágenes, la procedencia y autoría, su relación con el texto y la publicidad, los formatos y su distribución por secciones.The free press exerts a significant social impact, as it and carries its urgent informative function through hard-hitting headlines, punchy articles and news briefs. Distributed at key transportation facilities, coffee shops, cultural centers and libraries, these unique media and the photography they contain have not been extensively studied to date. This paper provides an exhaustive study of the application and use of photography in three nationally distributed free daily newspapers (20 Minutos, Qué and Adn. The study assesses the number of pictures published, their origins or authors, the relationship between photo and text, the use of photos in advertising, as well as formats and photo distribution within newspaper's sections.

  19. Utilisation de l'essai comete et du biomarqueur gamma-H2AX pour detecter les dommages induits a l'ADN cellulaire par le 5-bromodeoxyuridine post-irradiation

    Science.gov (United States)

    La Madeleine, Carole

    Ce memoire est presente a la Faculte de medecine et des sciences de la sante de l'Universite de Sherbrooke en vue de l'obtention du grade de maitre es sciences (M.Sc.) en radiobiologie (2009). Un jury a revise les informations contenues dans ce memoire. Il etait compose de professeurs de la Faculte de medecine et des sciences de la sante soit : Darel Hunting PhD, directeur de recherche (departement de medecine nucleaire et radiobiologie), Leon Sanche PhD, directeur de recherche (departement de medecine nucleaire et radiobiologie), Richard Wagner PhD, membre du programme (departement de medecine nucleaire et radiobiologie) et Guylain Boissonneault PhD, membre exterieur au programme (departement de biochimie). Le 5-bromodeoxyuridine (BrdU), un analogue halogene de la thymidine reconnu depuis les annees 60 comme etant un excellent radiosensibilisateur. L'hypothese la plus repandue au sujet de l'effet radio sensibilisant du BrdU est qu'il augmente le nombre de cassures simple et double brin lorsqu'il est incorpore dans l'ADN de la cellule et expose aux radiations ionisantes. Toutefois, de nouvelles recherches semblent remettre en question les observations precedentes. Ces dernieres etudes ont confirme que le BrdU est un bon radiosensibilisateur, car il augmente les dommages radio-induits dans l'ADN. Mais, c'est en etant incorpore dans une region simple brin que le BrdU radiosensibilise l'ADN. Ces recherches ont egalement revele pour la premiere fois un nouveau type de dommages produits lors de l'irradiation de l'ADN contenant du BrdU : les dimeres interbrins. Le but de ces travaux de recherche est de determiner si la presence de bromodeoxyuridine dans l'ADN augmente l'induction de bris simple et / ou double brin chez les cellules irradiees en utilisant de nouvelles techniques plus sensibles et specifiques que celles utilisees auparavant. Pour ce faire, les essais cometes et la detection des foci H2AX phosphorylee pourraient permettre d'etablir les effets engendres par

  20. 纳米金属Cu对ADN热分解的影响%Effects of Nanometer Cu Powder on ADN Thermal Decomposition

    Institute of Scientific and Technical Information of China (English)

    梁寒曙; 刘冠鹏

    2014-01-01

    为了研究纳米Cu粉催化剂对NH4N(NO)2(ADN)热分解的影响,采用DSC和TG研究了纳米Cu粉作用下ADN的热分解.结果表明,纳米Cu对ADN热分解起到较好的催化作用,可以将ADN热分解峰温从212.6℃提前至126.3℃,降低了86.3℃;纳米Cu粉使ADN的热分解呈现出2个明显的热分解过程,但仅对其第一步分解过程有明显催化作用,而对ADN分解的中间产物AN的吸热分解没有明显影响.同时对纳米Cu粉催化ADN热分解的机理进行了分析.

  1. High Energy Composite Propellant Containing ADN and HNF at Abroad%国外含ADN或HNF的高能复合推进剂

    Institute of Scientific and Technical Information of China (English)

    李上文; 赵凤起; 罗阳; 高茵

    2004-01-01

    二硝酰胺铵(ADN)和硝仿肼(HNF)是两种新型氧化剂,它们与固体推进剂常用的氧化剂AP相比具有能量贡献更大(比冲大3~10s)、燃烧产物低特征信号、无污染等特点.ADN在推进剂中应用的技术关键是球形化和提高化学安定性.实验证明,以ADN为基的推进剂在工艺上可行;燃烧稳定,燃速较高,在6~55MPa压力范围内n=0.68;化学安定性和相容性满足要求,故ADN将是21世纪首选的AP替代物.荷兰人近年在HNF的合成改进和纯度的提高方面取得很大进展,很大程度上克服了HNF安定性和相容性差的缺点,可以用复合推进剂的常用工艺制造出HTPB/HNF推进剂样品,并测出基本性能,但力学和长贮性能尚有待改进.

  2. High Efficient Undoped Blue Organic Light-emitting Diodes Based on ADN%基于ADN的非掺杂高效蓝色OLED器件

    Institute of Scientific and Technical Information of China (English)

    张刚; 姜文龙; 汪津; 王立忠; 王广德; 丁桂英

    2009-01-01

    采用真空蒸镀的方法,制备了以ADN为发光层的高效率非掺杂蓝色有机电致发光器件.器件的结构为ITO/2T-NATA(15 nm)/NPBx(15 nm)/ADN(25+d nm)/BCP(8 nm)/ Alq_3(30 nm)/LiF(0.5 nm)/Al.通过调整ADN层的厚度,研究了器件的发光性能.测试结果表明,器件在6 V电压时电流效率达到最大,为2.77 cd/A;在16 V时亮度达到最大,为7 227 cd/m~2.当ADN的厚度为30 nm、器件的电压从5 V变化到16 V时,色坐标在(0.21,0.32)至(0.19,0.29)之间,均在蓝光区域.

  3. Comparación de cuatro métodos de restauración del adn en muestras de plasma y láminas de citología cérvico-uterina como una herramienta para mejorar la calidad de la muestra

    OpenAIRE

    Marquez, Pilar caterin

    2011-01-01

    Objetivos: comparar cuatro métodos de restauración del adn en plasma y láminas cérvico-uterinas como una herramienta para mejorar la calidad de la muestra. Métodos: a 20 muestras de plasma sanguíneo y 20 muestras de láminas citológicas, se les realizó aislamiento de adn mediante kit comercial y fenol-cloroformo. A todas las muestras se les realizó un tratamiento pre-pcr con cuatro diferentes tipos de actividad de adn polimerasa: 1. Exonucleasa y endonucleasa 5’-3’. 2. Exonucleasa 5’-3’. 3. Kl...

  4. Genética y Arqueología : Análisis molecular de ADN procedente de restos esqueléticos.

    Directory of Open Access Journals (Sweden)

    N. Izaguirre

    1998-01-01

    Full Text Available La recuperación de moléculas de ADN mitocondrial (ADNmt de   muestras esqueléticas de diversa antigüedad, permite estudiar la   variabilidad genética de los grupos humanos del pasado. El análisis del ADNmt en tejido antiguo presenta una ventaja fundamental frente al nuclear, debido a su alto número de copias por célula. Los principales problemas metodológicos descritos en este tipo de   análisis, han podido resolverse en aquellos casos en que las   muestras presentaban buenas condiciones de preservación. Estos han sido fundamentalmente : 1.- inhibición de la amplificación,2.- autentificación de los resultados y 3.- detección y   eliminación de la contaminación. La variabilidad en el éxito de la amplificación entre individuos de un mismo yacimiento, indica que las condiciones individuales de cada inhumación son más importantes que la antigüedad. Por otro lado, las piezas dentarias han demostrado ser el material más adecuado para el estudio del ADNmt antiguo. La interpretación de los datos a nivel poblacional sólo es posible en aquellos yacimientos que presentan un amplio número de individuos, dado el relativamente bajo rendimiento de la metodología.

  5. Desarrollo de dos cepas atenuadas de "Salmonella enterica serovar choleraesuis" como vehículos para vacunas de ADN y expresión de un antígeno modelo

    OpenAIRE

    Bartolomé Ayarza, Almira

    2011-01-01

    En el campo de la sanidad animal la estrategia más rentable y segura en la profilaxis de las enfermedades infecciosas consiste en el empleo de vacunas. Una de las estrategias más económicas y eficaces para desarrollar estas vacunas es el uso de cepas vacunales vivas atenuadas. Estas cepas presentan como ventaja añadida la posibilidad de ser utilizadas como vehículos vacunales, tanto para liberar vacunas de ADN (que se ha denominado recientemente como bactofección) como para expresar antígenos...

  6. Experiencia clínica con una prueba de diagnóstico antenatal no invasivo con ADN fetal libre para trisomías 21, 18, y 13, en población general

    OpenAIRE

    Vera P-G.,Claudio; Carvajal C,Jorge

    2013-01-01

    Objetivo: Evaluar la prueba antenatal no invasiva (NIPT) de ADN fetal libre de células como método de cribado para las trisomías 21, 18, y 13 en práctica clínica obstétrica. Método: Estudio observacional de mujeres embarazadas que se sometieron a pruebas de cribado antenatal para trisomía fetal desde el 30 de julio de 2012 al 1 de diciembre de 2012. NIPT se ofreció a todas las pacientes, además del cribado combinado de primer trimestre (FTS). Resultados: La cohorte incluyó a 289 mujeres con u...

  7. Algoritmos de identificación de biclústeres con evolución coherente en microarreglos de ADN [Algorithms for discover coherent evolution biclusters on DNA microarrays

    OpenAIRE

    Peña Paz, Lyda; Facultad de Ingeniería, Universidad Autónoma de Occidente (Cali, Valle, Colombia)

    2016-01-01

    El Biclustering es una técnica empleada para el análisis de microarreglos de ADN, con el objetivo de identificar subgrupos de genes y de condiciones que muestren patrones similares de comportamiento. Existen diferentes tipos de biclústeres, siendo los de evolución coherente uno de los más difíciles de identificar debido a que no se consideran los valores exactos de los niveles de expresión sino el sentido en que se mueven. En este trabajo se presenta inicialmente un resumen de los algoritmos ...

  8. Catálogo de las muestras de fauna de la Comunidad de Madrid conservadas en la Colección de Tejidos y ADN del Museo Nacional de Ciencias Naturales

    OpenAIRE

    Rey Fraile, Isabel; Álvarez Dorda, Beatriz

    2006-01-01

    [ES] Este trabajo presenta el catálogo de muestras conservadas en la colección de Tejidos y ADN de las especies animales (vertebrados e invertebrados) de la Comunidad de Madrid. Se proporcionan los porcentajes frente al total de las muestras conservadas en la colección y las clases animales representadas. Esta información pone de manifiesto el esfuerzo que el Museo Nacional de Ciencias Naturales realiza para conservar a nivel genómico el mayor porcentaje posible de la diversidad actual de la ...

  9. El diseño ciberperiodístico español: análisis de los casos de Vilaweb y ADN

    Directory of Open Access Journals (Sweden)

    David Parra

    2008-12-01

    Full Text Available ResumenEl proceso diacrónico del diseño ciberperiodístico español atraviesa tres etapas: la mera réplica online de las publicaciones en papel, característico de la segunda mitad de los años noventa; la apuesta por ejes directrices como multimedialidad e interactividad, propia de los ciberperiódicos de la primera mitad de la primera década del siglo XXI; y la situación actual, en la que se profundiza en los rasgos anteriores y se incide en nuevos formatos como la telefoníamóvil. Para verificar la realidad de dichas tendencias hemos procedido al análisis de dos medios paradigmáticos del ciberperiodismo español: Vilaweb (el primero en apostar por una realidad como Internet y ADN (pionero en la adaptación de su diseño a realidades emergentes como Web 2.0 y Periodismo 3.0. La realización de dicho análisis se ha basado en el estudio de los elementos anteriormente indicados y su aplicación a las páginas web de estos cibermedios.Además el examen de estos dos casos permitirá comprender mejor este conjunto de tendencias a corto, medio y largo plazo.AbstractThe diachronic process of Spanish cyberjournalism design crosses over three steps: a mere online copy of newspapers, typical of the second half of the nineties; the strengthening of strategic axis like multimedia and interactivity, on the first half of the first decade of 21st century; and nowadays, making deeper all of these features and searching new formats like mobile telephony. In order to verify the truth of these tendencies, two of the paradigmatic Spanish cybermedia have been studied: Vilaweb (the first one to incorporate to Internet and AND (a pioneer in the adaptation of its design to items like Web 2.0 or Cyberjournalism 3.0. This analysis is based in these elements and their application to the web sites of the two cybermedia. The study of these two case stories will also allow us to understand in a better way this group of short, middle and long term tendencies.

  10. Evaluación del ADN espermático de llamas utilizando azul de toluidina Evaluation of llama sperm dna using toluidine blue

    Directory of Open Access Journals (Sweden)

    M.I Carretero

    2009-06-01

    Full Text Available El colorante azul de toluidina (AT se une al ADN permitiendo diferenciar espermatozoides de acuerdo al grado de condensación de la cromatina. Los objetivos de este trabajo fueron: poner a punto una técnica que evalúe la condensación de la cromatina espermática de llama, determinar los patrones de condensación para la especie mediante la tinción con AT y determinar si es posible utilizar ditíotreitol (DTT como control positivo de la tinción. Se ensayaron 2 tiempos de fijación de las muestras con etanol 96 º (2 y 30 minutos y 3 tiempos de incubación con DTT al 1% (30 seg, 1,5 min y 3 min. Los patrones de coloración observados fueron: coloración celeste (negativos, sin alteración en la condensación normal de la cromatina, violeta claro (intermedios, algún grado de descondensación, violeta oscuro (positivos, alto grado de descondensación. No se observaron diferencias significativas entre los tiempos de fijación tanto en las muestras con y sin DTT. En conclusión, se logró simplificar la técnica de AT y determinar los diferentes patrones en espermatozoides de llama. Se comprobó que la incubación con DTT se puede utilizar como control positivo de la técnica y para evaluar la susceptibilidad de cada individuo a la descondensación in vitro.Toluidine blue stain (TB binds to DNA, allowing differentiation of spermatozoa according to the degree of chromatin condensation. The objectives of this study were to adapt a technique for evaluating sperm chromatin in llamas, determine chromatin condensation patterns in llamas using TB and determine if it is possible to use dithiothreitol (DTT as a positive control for the stain. Two fixation times with ethanol 96° (2 and 30 minutes and 3 incubation times with 1% DTT (30 s, 1.5 min and 3 min were studied. Staining patterns observed were: light blue (negative, without alteration in the normal chromatin condensation, light violet (intermediate, some degree of decondensation, dark violet

  11. ADN antiguo; restos esqueletales precolombinos

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    Dejean, Cristina Beatriz

    2005-01-01

    Full Text Available El objetivo de este estudio consistió en: 1 determinar haplogrupos de ADNmt en restos esqueletales precolombinos de Pampa Grande, Prov. de Salta, Argentina, y 2 comparar los resultados obtenidos respecto de los registrados en poblaciones nativas extintas y actuales de la Argentina. A partir de muestras de hueso compacto de 21 individuos se logró, en 16 de ellos (76%, extraer, amplificar y secuenciar la región hipervariable I del ADNmt. Se observaron 17 sitios polimórficos y los porcentajes de los haplogrupos fueron los siguientes: A=6%, B=50%, C=0% y D =44%. Las elevadas frecuencias de los haplogrupos B y D son concordantes con las detectadas en poblaciones aborígenes actuales de la Argentina (B=38%, D=32%. El dendrograma obtenido revela que Pampa Grande se une al conjunto de las poblaciones del Chaco (Chorote, Toba, Chulupi y a los mapuche de origen andino y se separa de los grupos extintos de Tierra del Fuego, probablemente, por la ausencia en estos de los haplogrupos A y B. Se discuten posibles causas determinantes de las relaciones y afinidades biológicas observadas.

  12. Medicina genómica: Aplicaciones del polimorfismo de un nucleótido y micromatrices de ADN Genomic Medicine: Polymorphisms and microarray applications

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    Monica P. Spalvieri

    2004-12-01

    Full Text Available Esta actualización tiene por objeto difundir un nuevo enfoque de las variaciones del ADN entre individuos y comentar las nuevas tecnologías para su detección. La secuenciación total del genoma humano es el comienzo para conocer la diversidad genética. La unidad de medida reconocida de esta variabilidad es el polimorfismo de un solo nucleótido (single nucleotide polymorphism o SNP. El estudio de los SNPs está restringido a la investigación pero las numerosas publicaciones sobre el tema hacen vislumbrar su entrada en la práctica clínica. Se presentan ejemplos del uso de SNPs como marcadores moleculares en la genotipificación étnica, la expresión génica de enfermedades y como potenciales blancos farmacológicos. Se comenta la técnica de las matrices (arrays que facilita el estudio de múltiples secuencias de genes mediante chips de diseño específico. Los métodos convencionales analizan hasta un máximo de 20 genes, mientras que una sola micromatriz provee información sobre decenas de miles de genes simultáneamente con una genotipificación rápida y exacta. Los avances de la biotecnología permitirán conocer, además de la secuencia de cada gen, la frecuencia y ubicación exacta de los SNPs y su influencia en los comportamientos celulares. Si bien la validez de los resultados y la eficiencia de las micromatrices son aún controvertidos, el conocimiento y caracterización del perfil genético de un paciente impulsará seguramente un cambio radical en la prevención, diagnóstico, pronóstico y tratamiento de las enfermedades humanas.This update shows new concepts related to the significance of DNA variations among individuals, as well as to their detection by using a new technology. The sequencing of the human genome is only the beginning of what will enable us to understand genetic diversity. The unit of DNA variability is the polymorphism of a single nucleotide (SNP. At present, studies on SNPs are restricted to basic research

  13. REPARACIÓN DEL ADN: UNA POSIBLE RELACIÓN ENTRE LA DEFICIENCIA DE FOLATO Y LA MUERTE NEURONAL DNA Repair: A Link Between Folate Deficiency and Neuronal Cell Death

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    GONZALO ARBOLEDA

    2007-11-01

    Full Text Available El presente artículo explora el papel que desempeña el folato como conocido metabolito del ciclo de un carbono (OCM, del inglés onecarbon metabolism en la alteración de la integridad de las células nerviosas. Aquí se discute evidencia reciente de la literatura que muestra la reparación del ADN como un proceso relacionado con la apoptosis neuronal inducida por ausencia de folato.This essay explores the role of folate in disruption of neural cell integrity. Here, it is discussed recent evidence which shows DNA reparation as a process related to neuronal apoptosis induced by folate depletion.

  14. LE RÔLE DES PROTÉINES BRCA1 ET BRCA2 DANS LA RÉPARATION DES ALTÉRATIONS MOLÉCULAIRES DE L’ADN

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    Lucian Negura

    2007-08-01

    Full Text Available Les gènes BRCA1 et BRCA2 sont des suppresseurs tumoraux dont les phénotypes mutants prédisposent au cancer mammaire et ovarien. Ce sont des gènes nouveaux et peu d’informations concernant leurs fonctions ont été apportées par leur séquence. Cependant, de nombreuses études sur les protéines BRCA et leurs partenaires moléculaires ont montré l’implication dans une multitude de processus cellulaires fondamentaux, incluant la réponse cellulaire aux altérations de l’ADN. Nous présentons quelques unes des plus récentes connaissances dans ce domaine.

  15. ESTANDARIZACIÓN DE LA EXTRACCIÓN DE ADN Y VALIDACIÓN DE LA PCR MÚLTIPLE PARA DETECTAR Listeria monocytogenes EN QUESO, LECHE, CARNE DE RES Y POLLO

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    M. Mercado

    2005-12-01

    Full Text Available Este trabajo validó la técnica de PCR para la detección de L. monocytogenes a partir de ADN de cultivos puros y en muestras de leches crudas, quesos frescos, carne de res y carne de pollo. En DNA de cultivos puros la sensibilidad, la especificidad y la reproducibilidad encontrada fue de 100%, 101UFC/ml y K=1,respectivamente. Para la extracción del ADN de los alimentos se emplearon dos métodos, el primero, basado en la precipitación alcohólica en presencia de NaI, lo que redujo en gran medida las grasas; permitiendo la detección directa de 10 1 UFC/ml en leche cruda y 105 UFC/g en queso fresco. El segundo método, basado en la extracción con lisozima, proteinasa K y fenol-cloroformo, permitió establecer límites de detección de 102 y 104 UFC/g para las carnes de res y pollo respectivamente. La PCR se basó enla especificidad de los iniciadores LI1/U1 que amplificaron una banda de 938 pb (identificación de género característica del rDNA 16S y los iniciadores LF/LR que amplificaron una banda de 750 pbcaracterística del gen hlyA (identificación de especie. Los resultados de la validación reportaron con relación al método “Gold Standard” una reproducibilidad, sensibilidad y especificidad del 100% en leches crudas. Para las muestras de queso frescos se reportó una reproducibilidad de 97%, sensibilidad 96.3% y especificidad del 100%, para la carne de pollo la reproducibilidad fue 98.43%, sensibilidad96.9%, especificidad 100%, valor predictivo positivo 100% y valor predictivo negativo 100%, para la carne de res todos los parámetros fueron 100%. El método “Gold Standard” reportó 100% para todos losparámetros. El trabajo muestra que ambas técnicas pueden ser utilizadas para detectar L. monocytogenes en este tipo de alimentos y que la PCR reduce el tiempo de ensayo considerablemente.

  16. Deteccção do DNA de Mycobacterium leprae em secreção nasal Detección del ADN de Mycobacterium leprae en secreción nasal Detection of Mycobacterium leprae DNA in nasal swab

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    Ana Rosa Botelho Pontes

    2008-11-01

    Full Text Available Estudos têm demonstrado alta sensibilidade da técnica da reação em cadeia de polimerase (PCR na identificação do DNA do Mycobacterium leprae. Este estudo objetivou avaliar a sensibilidade da PCR na detecção do DNA do M. leprae em "swab" nasal de pacientes hansenianos e comparar os resultados com a baciloscopia e formas multibacilares (MBs e paucibacilares (PBs. Foram coletadas amostras de secreção nasal de 24 pacientes hansenianos, conservadas em solução de lise um e dois. Os resultados da PCR foram altamente significativos (pLos estudios han demostrado una alta sensibilidad de la técnica de Reacción en Cadena de la Polimerasa (PCR para identificar el ADN de Mycobacterium leprae. El objetivo del estudio fue evaluar la sensibilidad de la PCR en la detección de ADN de M. leprae en hisopo nasal de los pacientes hansenianos y comparar los resultados con la baciloscopía y las formas multibacilares (MBs y paucibacilares (PBS. Se obtuvieron muestras de secreción nasal de 24 pacientes hansenianos, conservados en solución de lisis uno y dos. Los resultados de la PCR fueron muy significativas (p Studies have demonstrated high sensibility of the polimerase chain reaction (PCR technique in the identification of the Mycobacterium leprae DNA . This study aimed to evalue the PCR sensibility at the detection of the M. leprae DNA in nasal swab of leprosy patients and to compare the results with the bacilloscopy and multibacillary (MBs and paucibacilares (PBs forms. Nasal secretion samples of 24 leprosy patients were collected, and were preserved in one and two lise's solution. The PCR results were highly significant (p <0.0000 and they revealed grater sensibility than bacilloscopy, in several clinical forms. Nevertheless, still different studies are necessary, testing new markers and preservatives, with the purpose of lifting up the sensibility of this technique, in nasal secretion samples.

  17. Detection of Brucella melitensis DNA in the milk of sheep after abortion by PCR assay Detección de ADN de Brucella melitensis mediante la prueba de PCR en muestras de leche de ovejas postaborto

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    Z Ilhan

    2008-01-01

    Full Text Available Laboratory diagnosis of brucellosis is generally performed by microbiological and serological methods. PCR assay is a specific and sensitive choice for the detection of different bacterial agents. An evaluation of this test was carried out for the detection of Brucella melitensis DNA in sheep milk. 102 milk samples from sheep after abortion were taken and studied using bacteriological culture, PCR and milk ring test (MRT. PCR found B. melitensis DNA in 24 (23.5% out of 102 milk samples, while only 8 (7.8% of the samples were positive to B. melitensis through direct culture. MRT found 28 (27.4% positive milk samples. The detection limit for PCR in sheep milk inoculated with B. melitensis strain 16 M was 1.7x10³-1.7x10(4 cfu/ml. PCR and MRT coincidence was 96%. The diagnostic sensitivity and specificity were determined as 100% and 81.3% respectively for PCR assay and 75% and 75% for MRT. PCR is a useful tool for a fast diagnosis of B. melitensis in sheep milk.El diagnóstico de laboratorio de brucelosis es generalmente realizado por métodos microbiológicos y serológicos. La prueba PCR es reconocida como una alternativa específica y sensible para la detección de diferentes agentes bacterianos. Se realizó una evaluación de la prueba PCR para la detección de ADN de Brucella melitensis en leche de oveja. Ciento dos muestras tomadas de ovejas postaborto fueron analizadas por métodos de cultivo bacteriológico, prueba PCR y prueba del anillo en leche (MRT. El PCR detectó ADN de B. melitensis en 24 (23,5% de 102 muestras de leche, mientras que solamente 8 (7,8% muestras de leche fueron positivas a B. melitensis por cultivo directo. El MRT detectó 28 (27,4% muestras positivas de leche. El límite de detección de B. melitensis 16 M por PCR fue de 1,7x10³-1,7x10(4 ufc/ml en leche inoculada. La concordancia entre PCR y MRT fue 96%. La sensibilidad diagnóstica y la precisión fueron determinadas como el 100% y el 81,3% respectivamente para la

  18. 纳米铝粉的固相化学还原法制备、表征及对ADN热分解性能的影响%Solid Phase Chemical Reduction Synthesis and Characterization of Aluminum Nanopowders and the Effect on the Thermal Decomposition of ADN

    Institute of Scientific and Technical Information of China (English)

    李鑫; 赵凤起; 罗阳; 高红旭; 姚二岗; 姜菡雨

    2015-01-01

    以山梨糖醇酐三油酸酯(Span-85)作为过程控制剂,通过氢化铝锂还原无水氯化铝,采用高能机械球磨法制备了纳米铝粉(n-Al).利用扫描电镜(SEM)、高分辨率透射电镜(TEM)、X射线衍射(XRD)仪、傅里叶变换红外光谱(FTIR)仪及X射线光电子能谱(XPS)仪对其形貌和结构进行了表征.用差示扫描量热仪(DSC)对ADN(二硝酰胺铵)、n-Al/ADN的热分解反应特性进行了研究.结果表明:纳米铝粉属立方晶系,表面包覆有无定型Al2O3氧化物及部分表面活性剂Span-85,同时制备的纳米铝粉试样中含少量AlCl3·6H2O杂质;此方法制备的n-Al粒子对ADN液化温度几乎没有影响,但放热分解温度明显增大,且ADN分解由多重峰变为单一的尖峰.%Aluminum nanopowders (n-Al) were synthesized via reduction of aluminum chloride anhydrous by lithium aluminum hydride with a mechanical ball-milling method,using Span-85 as process control agent.The surface morphologies and structures were characterized by scanning electron microscope (SEM),high resolution transmission election microscope (TEM),X-ray diffraction (XRD) instrument,Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS).The thermal decomposition process of ADN and composite system n-Al/ADN were investigated using differential scanning calorimetry (DSC).The results show that aluminum nanopowders belong to the cubic crystal system,and their surface is coated with amorphous oxide A12O3 and surfactant Span-85.At the same time,the samples contain a small amount of impurities A1C13·6H2O.Aluminum nanopowders have little influence on the liquefaction temperature of ADN,but the decomposition temperature increases significantly,and the decomposition of ADN becomes from multiple peak into a single spike.

  19. Catálogo de las muestras de fauna de la Comunidad de Madrid conservadas en la colección de Tejidos y ADN del Museo Nacional de Ciencias Naturales

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    Rey, I.

    2006-05-01

    Full Text Available This work presents the catalogue of preserved samples of animal species (vertebrate and invertebrate from Comunidad de Madrid kept in the Tissues and DNA Collection of the Museo Nacional de Ciencias Naturales. Percentages are shown, calculated against the total number of samples and animal class kept in the collection. This information shows the effort developed by the Museo Nacional de Ciencias Naturales to preserve at the genomic level the greatest possible level of genomic biodiversity in Comunidad de Madrid.

    Este trabajo presenta el catálogo de muestras conservadas en la colección de Tejidos y ADN de las especies animales (vertebrados e invertebrados de la Comunidad de Madrid. Se proporcionan los porcentajes frente al total de las muestras conservadas en la colección y las clases animales representadas. Esta información pone de manifiesto el esfuerzo que el Museo Nacional de Ciencias Naturales realiza para conservar a nivel genómico el mayor porcentaje posible de la diversidad actual de la Comunidad de Madrid.

  20. Evaluación de la unión espermatozoide-ADN exógeno en espermatozoides porcinos eyaculados y epididimarios Evaluation of binding sperm-exogenous DNA in ejaculate and epididimary porcine spermatozoa

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    FA García-Vázquez

    2009-01-01

    Full Text Available La transgénesis es una potente herramienta biotecnológica para la generación de animales modificados genéticamente con aplicaciones en diversas áreas como veterinaria, biomedicina y agricultura. En 1989 se describe un nuevo método para la producción de animales transgénicos, la transgénesis mediada por espermatozoides (SMGT. Este método está basado en la habilidad intrínseca de las células espermáticas para unir ADN exógeno y permitir su transferencia al interior de los ovocitos. El objetivo de este estudio fue comparar la capacidad de transferencia de ADN exógeno que presenta el espermatozoide eyaculado libre de plasma seminal vs epididimario, así como la viabilidad y cinética de unión mediante la citometría de flujo. Los resultados que obtuvimos mostraron que los espermatozoides epididimarios presentan una capacidad similar a los eyaculados y centrifugados tanto en el grado de unión al ADN (12,63 ± 1,23% vs 10,94 ± 1,05%, P = 0,31 como en la viabilidad (espermatozoides no viables 14,64 ± 0,94% vs 13,42 ± 0,61%, P = 0,23 a lo largo del tiempo de incubación. La mayor parte de dicha unión tiene lugar en los espermatozoides no viables para ambos tipos de espermatozoides (10,53 ± 1,01% vs 9,89 ± 0,97%, P = 0,98. En relación a la cinética de unión, observamos que para ambos grupos experimentales el mayor porcentaje de unión con el transgén ocurre en los primeros 15 minutos de coincubación, a partir del cual la unión se mantiene más o menos constante hasta los 120 minutos. Con este estudio hemos demostrado que tanto espermatozoides epididimarios como eyaculados son capaces de interactuar con el ADN exógeno, por lo que se podría utilizar la SMGT combinada con técnicas de reproducción asistida como la ICSI para la obtención de embriones y lechones transgénicos.Transgenic biotechnology is a powerful tool for the generation of genetically modified animals with applications in various fields such as veterinary

  1. Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban triatominae Optimización de la técnica de ADN polimórfico amplificado al azar (RAPD para su utilización en la caracterización genética de triatomíneos cubanos

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    Jorge Fraga

    2005-10-01

    Full Text Available Random amplified polymorphic DNA (RAPD technique is a simple and reliable method to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of assay. In this study, we analyzed the effect of changing the concentration of primer, magnesium chloride, template DNA and Taq DNA polymerase with the objective of determining their optimum concentration for the standardization of RAPD technique for genetic studies of Cuban Triatominae. Reproducible amplification patterns were obtained using 5 pmoL of primer, 2.5 mM of MgCl2, 25 ng of template DNA and 2 U of Taq DNA polymerase in 25 µL of the reaction. A panel of five random primers was used to evaluate the genetic variability of T. flavida. Three of these (OPA-1, OPA-2 and OPA-4 generated reproducible and distinguishable fingerprinting patterns of Triatominae. Numerical analysis of 52 RAPD amplified bands generated for all five primers was carried out with unweighted pair group method analysis (UPGMA. Jaccard's Similarity Coefficient data were used to construct a dendrogram. Two groups could be distinguished by RAPD data and these groups coincided with geographic origin, i.e. the populations captured in areas from east and west of Guanahacabibes, Pinar del Río. T. flavida present low interpopulation variability that could result in greater susceptibility to pesticides in control programs. The RAPD protocol and the selected primers are useful for molecular characterization of Cuban Triatominae.La técnica de ADN polimórfico amplificado al azar (RAPD es un método simple para detectar el polimorfismo genético del ADN. Diferentes factores afectan los perfiles de amplificación lo que se manifiesta en la presencia de bandas falsas y en la reproducibilidad del ensayo. En nuestro trabajo analizamos los cambios de la concentración de cebador, ADN molde, cloruro de magnesio y de Taq ADN polimerasa con el objetivo de determinar su

  2. DNA aneuploidy in colorectal adenomas: Role in the adenoma-carcinoma sequence Aneuploidía del ADN en adenomas colónicos: Papel en la secuencia adenoma-carcinoma

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    M. Alcántara Torres

    2005-01-01

    ímetros, recogidos de forma consecutiva de 54 pacientes. Las piezas se obtuvieron en todos los casos mediante resección endoscópica o quirúrgica. En 49 casos se trataba de adenomas con displasia de bajo grado, en dos casos de adenomas con displasia de alto grado, dos adenocarcinomas intramucosos y en otros cuatro de adenocarcinomas microinvasivos. El estudio del ADN se realizó en la pieza operatoria en fresco mediante citometría de flujo utilizado el método de Vindelov. Resultados: se detectó ADN aneuploide en cinco de los 49 adenomas con displasia de bajo grado (10%, en los cuatro adenomas con displasia de alto grado o adenocarcinomas intramucosos (100% y en tres de los cuatro adenocarcinomas microinvasivos (75%. Se observó asociación significativa entre el hallazgos de aneuploidía y displasia de alto grado, adenocarcinoma intramucoso o microinvasivo (p < 0,001. No se apreció asociación entre la existencia de aneuploidía y la edad de los pacientes, sexo, sintomatología clínica, tamaño ni localización de los adenomas. Conclusiones: en adenomas colónicos la incidencia de aneuploidía fue del 10% cuando se trataba de adenomas con displasia de bajo grado y del 87% cuando presentaban displasia de alto grado o adenocarcinoma siendo la diferencia estadísticamente significativa. Estos hallazgos sugieren que la aneuploidía juega un papel en la secuencia adenoma-carcinoma.

  3. Non invasive prenatal diagnosis: analysis of circulating fetal DNA and cells in maternal blood El diagnóstico prenatal no invasor: análisis de células y ADN fetal circulantes en la sangre materna

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    Diana Cecilia Jaramillo Posada

    2009-11-01

    Full Text Available

    Prenatal non invasive diagnosis by means of analyses of foetal DNA or cells circulating in maternal blood is one of the most promising areas of obstetrics. Among maternal diseases that could be diagnosed by these methods, or whose behaviour could be predicted, are preeclampsia, growth restriction and preterm labour. Some foetal conditions that could be detected are sex, chromosomal anomalies and single-gene defects. However, these are complex and expensive techniques that are not regularly performed in health care institutions. With this review we intend to provide the readers with up to date information on the main techniques available for the study of circulating foetal cells and DNA, and on their possible clinical applications. The review was based on a search for journals indexed up to 2008 in Pubmed, Scielo and Latindex. Especially relevant articles were chosen by the authors.

    El diagnóstico prenatal temprano y no invasor por medio del análisis de células o ADN fetales circulantes en la sangre materna es un área prometedora de la obstetricia moderna. Entre las enfermedades que se pueden diagnosticar o cuyo comportamiento es posible predecir por estos métodos se encuentran la preeclampsia, la restricción del crecimiento intrauterino y el parto pretérmino. Algunas condiciones fetales que podrían detectarse son el sexo, ciertas anomalías cromosómicas y los defectos de un solo gen. Sin

  4. Detección de secuencias específicas de ADN de Spongospora subterranea en suelo y tubérculos de papa Detection of DNA specific sequences of Spongospora subterranea in soil and potato tubers

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    Ángel Díaz Jorge Evelio

    2004-07-01

    Full Text Available Con el fin de identificar de manera precoz el agente causal de la sarna polvosa de la papa (Spongospora subterranea fs subterranea tanto en semillas como en suelos aptos para el cultivo de este tubérculo, se ha desarrollado una prueba basada en la reacción en cadena de la polimerasa (PCR para la detección de tres secuencias ITS (espaciadores de transcripción interna específicos del ADNr de 372,390 y 391 pb presentes en el genoma de S. subterranea. Para ello se estandarizó una metodología de extracción y purificación de ADN del microorganismo a partir de tejido vegetal infectado (agallas de raíz de papa y pústulas en papa obtenido en pruebas de propagación del patógeno en invernadero. Una vez optimizada la PCR y determinada su sensibilidad, se validó la metodología molecular examinando muestras de tejido vegetal infectado y suelo infestado por el patógeno, provenientes de los departamentos de Cundinamarca y Nariño. La PCR detectó ADN del microorganismo tanto en el material vegetal infectado, como en las muestras de suelo analizadas (las 30 submuestras del área experimental fueron PCR positivas. Estos resultados muestran que dicha metodología se presenta no sólo como una técnica útil para la detección rápida del patógeno en suelo, sino también como una herramienta para determinar la presencia del microorganismo en suelos declarados libres de éste y como una alternativa eficaz para el control de calidad en la producción de semilla certificada de papa libre del patógeno. Palabras clave: sarna polvosa, quistosoros, espaciador de transcripción interna, PCR, plasmodiofóridos.A test has been developed for early identification of the casual agent for potato powdery scab (Spongospora subterranea fs subterranea. Identification was carried out in seeds and soil where this tuber is grown. A polymerase chain reaction (PCR was set up for detecting 372, 390 and 391 bp ribosomal DNA internal transcribed spacer sequences (ITS in

  5. La ADN topoisomerasa tipo I de protozoos patógenos como Diana terapéutica de fármacos antitumorales Type I DNA topoisomerase from protozoan pathogens as a potential target for anti-tumoral drugs

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    Rosa M Reguera

    2007-12-01

    Full Text Available La utilización intensiva de fármacos antiparasitarios es la causa principal de la aparición de microorganismos parásitos multirresistentes en las regiones del planeta donde son precisamente endémicos. Los agentes etiológicos de las denominadas enfermedades tropicales -malaria, criptosporiodiosis, enfermedad del sueño, enfermedad de Chagas o los distintos tipos de leishmaniosis- son protozoos unicelulares sobre los que no se ha desarrollado en la actualidad ninguna vacuna eficaz y cuyo tratamiento se basa en medidas sanitarias preventivas y en el uso de medicamentos. La quimioterapia antiparasitaria actual es cara, no está ausente de efectos adversos y no supone beneficios a las empresas que la comercializan, por lo que la inversión en I & D es marginal comparada con la llevada a cabo para otros procesos patológicos de menor relevancia médica. La identificación de las ADN topoisomerasas como dianas farmacológicas se basa en los excelentes resultados obtenidos en los ensayos clínicos llevados a cabo con los derivados de la camptotecina en la terapia antitumoral. Las importantes diferencias estructurales entre las ADN topoisomerasas de tipo I de tripanosomas y leishmanias con respecto a sus homólogas de mamífero ha abierto un nuevo campo de investigación que combina las técnicas de biología molecular con la cristalización de proteínas para poder diseñar nuevos fármacos dirigidos específicamente a su inhibición. Revisamos aquí las características de estas nuevas dianas farmacológicas, así como los compuestos que en el momento están siendo utilizados para su inhibición en los agentes parasitarios que causan las principales enfermedades tropicales.The intensive use of antiparasitic drugs is the main cause of the emergence of multiresistant parasite strains on those regions where these parasites are endemic. The aetiological agents of the so-called tropical diseases viz. malaria, cryptosporidiosis, sleeping sickness

  6. Aplicación de una técnica de Cromatografía de Exclusión molecular para la purificación de ADN en plantas de Coffea sp. APPLICATION OF A TECHNIQUE OF MOLECULAR EXCLUSION CHROMATOGRAPHY FOR THE PURIFICATION OF DNA FROM Coffea sp. PLANTS

    Directory of Open Access Journals (Sweden)

    Ana María García Cepero

    2006-12-01

    Full Text Available Uno de los mayores inconvenientes en la extracción y purificación de biomoléculas a partir de plantas del género Coffea, es un alto contenido de polifenoles y compuestos tánicos. En el presente artículo se describe una metodología que permite obtener ADN de alta pureza. La extracción del ADN del homogeneizado de tejido foliar en siete genotipos de Coffea sp., se realizó mediante la técnica citada por Chaparro (1993 y su purificación se logró mediante cromatografía de exclusión molecular sobre una fase estacionaria de Sephacryl S-1000. Los resultados muestran que la alta eficiencia de separación de ARN degradado, proteínas, pigmentos y compuestos que absorben entre 220 y 300 nm, permiten obtener un ADN de alta pureza a juzgar por los datos espectrofotométricos y electroforéticos.One of the greatest difficulties in extracting and purifying biomolecules from plants in the genus Coffea is the high polyphenol and tannin contents. In this study a methodology is described that allows obtaining high purity DNA from leaf tissues of seven genotypes of Coffea sp. by means of the technique desribed by Chaparro (1993 and its further purification was achieved by molecular exclusion chromatography on Sephacryl S-1000 (Pharmacia. The results showed that the high separation efficiency of degraded RNA, proteins, pigments, and other compounds that absorb between 220 and 300 nm allowed obtaining high purity DNA as judged by the spectophometric and electroforetic data.

  7. Approche morphologique de la fragmentation de l'ADN radio-induite par immunomarquage anti-poly (ADP-ribose) polymérase (PARP) : étude de cultures d'oligodendrogliomes

    Science.gov (United States)

    Varlet, P.; Beuvon, F.; Cervera, P.; Averbeck, D.; Daumas-Duport, C.

    1998-04-01

    Poly (ADP-ribose) polymerase (PARP) is a nuclear enzyme encompassing two zinc finger motifs which specifically binds to radiation induced DNA strand breaks. We develop a new immuno-labelling of poly ADP-ribose which coupled together with the immunodetection of cells in cycle with MIB1, permits to detect and quantify the DNA fragmentation induced by radiations (Cesium137). This method, applied to organotypical cultures of human oligodendroglioma, submitted to radiation, a dose dependant nuclear signal. This one increased significantly in the presence of a radiosensitizer like iododeoxyuridine (IUDR 5 g/ml). This poly ADP-ribose immunodetection can be useful, to detect furtherly the individual radiosensitivity of human glioma. Les protéases “ICE-like" ou caspases, sont les homologues humaines du produit du gène ced-3 du ver Caenorhabditis elegans et sont activées lors des étapes précoces de l'apoptose. L'objectif de ce travail vise à déterminer dans quelle mesure l'inhibition de l'une d'entre elles, la caspase-3 est susceptible de modifier la sensibilité des cellules vis-à-vis de l'apoptose radioinduite. Des lymphocytes spléniques murins irradiés en présence de Ac-DVED-CHO un inhibiteur spécifique de la caspase-3 présentent un taux de particules hypodiploïdes radioinduites bien inférieur à celui des contrôles et une diminution drastique de la fragmentation internucléosomale de l'ADN. Toutefois, ni l'externalisation des phospholipides anioniques, autre marqueur spécifique de l'apoptose, ni la viabilité ne sont affectées.

  8. Identificacion de marcadores genéticos del agente causal del marchitamiento del clavel fusarium oxysporum f.sp. dianthi mediante amplificacion arbitraria de fragmentos polimórficos de adn

    Directory of Open Access Journals (Sweden)

    Arbeláez G.

    1998-12-01

    Full Text Available La técnica de Amplificación Arbitraria de Fragmentos Polimórficos de ADN (RAPD fue utilizada para identificar marcadores genéticos útiles para el desarrollo de un método diagnóstico para Fusarium oxysporum f.sp. dianthi, el agente etiológico de la enfermedad del marchitamiento del clavel. Con el fin de identificar fragmentos genéticos característicos de este patógeno, un total de 18 aislados diferentes, provenientes de diferentes lugares del mundo y 17 cepas de F. oxysporum de otras formas especiales fueron amplificadas utilizando 15 iniciadores diferentes. Aunque ninguno de los iniciadores empleados en este estudio amplificó una banda común a todas las formas especiales dianthi, el iniciador OPA 17 mostró un patrón de RAPD que permitió la identificación de cuatro grupos polimórficos dentro de este grupo taxonómico. Este mismo iniciador, permitió la discriminación entre aislados de Fusarium oxysporum f.sp. dianthi y cepas de F. oxysporum de otras formas especiales. No se observó una correlación directa entre el patrón de RAPD y las razas reportadas para F. oxysporum f.sp. dianthi, previamente determinadas mediante ensayos biológicos por otros grupos de investigadores. Los análisis de hibridación molecular con fragmentos escogidos de estos patrones de RAPD, permitieron el reconocimiento selectivo de los cuatro grupos descritos. Los fragmentos genómicos identificados, son candidatos para el desarrollo de un  sistema diagnóstico por PCR para este patógeno del clavel.

  9. EVALUACIÓN DE DOS MÉTODOS DE EXTRACCIÓN DE ADN A PARTIR DE BIOPSIAS FIJADAS EN FORMALINA Y EMBEBIDAS EN PARAFINA EN CONDICIONES NO ÓPTIMAS Evaluation of Two Methods DNA Extraction from Formalin-Fixed, Paraffin-Embedded Tissues on Non-Optimal Conditions

    Directory of Open Access Journals (Sweden)

    JAVIER ANDRÉS BUSTAMANTE

    2011-08-01

    Full Text Available Los tejidos de archivo son material de incalculable valor para estudios retrospectivos que requieran la aplicación de análisis moleculares. Existen múltiples métodos de extracción de ADN a partir de este tipo de muestras. No obstante, la mayoría de métodos toman mucho tiempo y los reactivos empleados contribuyen a la fragmentación del ADN. Con el objetivo de optimizar dos métodos de extracción de ADN a partir de tejidos embebidos en parafina en condiciones no óptimas, se seleccionaron 47 bloques en parafina que contenían biopsias de pleura, pulmón y pericardio correspondientes a 24 pacientes (66,6% hombres mayores de 18 años, con inflamación granulomatosa crónica, remitidos al Departamento de Patología, Hospital Universitario del Valle entre 2002 y 2007. Se realizaron 10 cortes a cada muestra y se sometieron a dos métodos de extracción de ADN: 1. convencional y 2. QIAamp-DNA mini kit®. La eficiencia del ADN fue valorada por espectrofotometría y amplificación del gen GAPDH. La concentración de ADN de las muestras extraídas por el método convencional fue de 65,52 ng/µL ±11,47 (promedio ± EE y la relación 260/280 varió entre 0,52 y 2,30. De las muestras extraídas por el método comercial, la concentración media de ADN fue 60,89 ng/µL ± 6,02, con una absorbancia que osciló entre 0 y 2,64. El ADN obtenido fue sometido a PCR, de 47 muestras extraídas por ambos métodos, 25 y 23 respectivamente amplificaron exitosamente el gen GAPDH. Los métodos usados para la obtención de ADN presentaron un desempeño similar, revelando así su potencial utilidad en estudios retrospectivos a partir de biopsias embebidas en parafina en condiciones inadecuadas.Paraffin wax embedded tissues are an invaluable material for retrospective studies requiring the application of molecular analysis. Multiple methods are available to extract DNA from these kind of samples. However, the most common methods are slow and the reagents often

  10. Cost-effectiveness of conventional cytology and HPV DNA testing for cervical cancer screening in Colombia Costo-efectividad de la citología y la tamización con pruebas de ADN-VPH para cáncer de cuello uterino en Colombia

    Directory of Open Access Journals (Sweden)

    Oscar Andrés-Gamboa

    2008-08-01

    Full Text Available OBJECTIVE: To assess cost-effectiveness of conventional cytology and HPV DNA testing for cervical-cancer screening in Colombia. MATERIAL AND METHODS: The National Cancer Institute of Colombia (NCIC in 2007 developed a Markov model on the natural history of cervical cancer; no screening, conventional cytology, and HPV DNA testing were compared. Only direct costs were used. Outcomes comprise cervical cancer mortality, years of life saved, and lifetime costs. Discounted incremental cost-effectiveness ratios were estimated and sensitivity analyses were conducted for key parameters. RESULTS: Depending on the screening strategy a 69-81% mortality reduction might be expected. The HPV DNA testing every five years is a cost-effective strategy (Incremental Cost-Effectiveness Ratio (ICER: USD$44/YLS if the cost per test is under USD$31. The effectiveness was sensitive to coverage and primarily to follow-up. CONCLUSIONS: HPV DNA testing is a cost-effective alternative for screening in Colombia. Not only high coverage but high follow-up rates are critical for successful screening programs.OBJETIVO: evaluar el costo-efectividad de la citología convencional y la prueba de ADN-VPH para tamización de cáncer cervical en Colombia. MATERIAL Y MÉTODOS: el Instituto Nacional de Cancerología de Colombia construyó en 2007 un modelo de Markov de historia natural del cáncer cervical. Se comparó "no tamización", citología convencional y prueba de ADN-VPH. Se utilizaron costos directos. Los desenlaces fueron mortalidad, años de vida ganados y costos. Se calcularon razones de costo-efectividad incremental. Se realizaron análisis de sensibilidad para parámetros clave. RESULTADOS: la mortalidad se redujo 69-81% según la estrategia. La tamización con ADN-VPH cada cinco años es costo-efectiva (ICER (Razón de Costo-Efectividad incremental por sus siglas en inglés: 44 dólares por año de vida saludable si los costos por prueba son menores a 31 dólares. La

  11. Comparación de estrategias del cribado primario de lesiones de alto grado y cáncer de cuello de útero en población de Córdoba: el papel de la citología cervical y la determinación de adn del virus del papiloma humano

    OpenAIRE

    Ilic, Igor

    2011-01-01

    El objetivo principal del estudio es comparar la eficacia de las técnicas de cribado del cáncer de cuello del útero habitualmente utilizadas en nuestro medio; citología cervical triple de Wied y detección del ADN de VPH, utilizadas como pruebas únicas ó conjuntamente en pacientes de nuestra población. Objetivo secundario es determinar la contribución relativa de la infección por distintos genotipos del VPH de alto riesgo oncogénico en el desarrollo de anormalidades histológicas CIN 2, CIN 3 y...

  12. 考虑两相表面反应的二硝酰胺铵点火研究%Research on ammonium dinitramide (ADN) ignition with two-phase subsurface reactions

    Institute of Scientific and Technical Information of China (English)

    段毅; 刘宇; 覃粒子; 温正

    2010-01-01

    为了研究固体推进剂二硝酰胺铵(ammonium dinitramide, ADN)点火过程中物理化学变化,建立了1个考虑两相(固相和气相)表面化学反应的点火模型.该模型基于固相与气相的质量守恒方程、组元连续方程、能量守恒方程及有限速率化学动力学方程而建立,并引入多组元系统状态方程封闭方程组.模型中包含35种组元,2个固相ADN总分解反应和166个气相细节(基元)化学反应,并使用温度函数表示物性参数进行计算.应用该点火模型对0.1 MPa下ADN在不同初始温度下点火延迟时间进行预测,计算结果与试验数据较吻合,说明该点火模型较准确地描述了ADN点火过程;计算表明,ADN点火延迟时间随初始温度升高而急剧缩短,且初始温度高于600 K时,温度存在一个短时间的降低过程;计算得到ADN完全燃烧产物为H2O(0.393)、N2(0.394)、O2(0.193)及极少量的NO(0.009),表明ADN是一种绿色无污染低特征信号推进剂.

  13. Daño al ADN en mujeres expuestas al humo de la leña en Chiapas, México DNA damage in women exposed to firewod fuel smoke , in Chiapas, México

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    Crispín Herrera-Portugal

    2009-12-01

    Full Text Available Actualmente alrededor de la cuarta parte de la población mexicana, entre 25 y 28 millones de habitantes, cocina con leña, Sin embargo, el humo de la leña contiene una amplia gama de sustancias tóxicas, entre ellas el monóxido de carbono (CO cuyo impacto en la salud de la población rural debe ser estudiado. Por esto, el potencial daño al ADN asociado con la exposición a CO de 30 mujeres que cocinaban con leña en Chiapas, México, fue evaluado por el ensayo cometa. Los resultados se compararon con 30 controles comparables en edad y condiciones socioeconómicas, quienes cocinaban con gas licuado de petróleo (GLP. Se obtuvieron muestras de sangre total para medir carboxihemoglobina (COHb y llevar a cabo el ensayo cometa. Se encontró diferencia significativa (PCurrently, about a quarter of the Mexican population, between 25 and 28 million people, cook with firewood. However, wood smoke contains a wide range of toxic substances, including carbon monoxide (CO whose impact on health of the rural population should be studied. Therefore, the potential DNA damage associated with the exposition to CO of 30 women who cooked with wood in Chiapas, Mexico, was assessed using Comet Assay. Results were compared with 30 controls of similar age and socioeconomic status, who cooked with liquefied petroleum gas (LPG. We obtained whole blood samples to measure carboxyhemoglobin (% COHb and perform the comet assay. There was a significant difference (P <0.001 in the percentages of COHb between women who cooked with wood (mean= 6.6% and those who did it with LPG (mean=1.8% being 3.6 times higher in the former compared with the latter. There was a significant difference in comet tail length between the two groups examined (mean 18.5 +/- 4.21 versus 5.97 +/- 1.0 μm, P <0.001 and tail moment (mean 4.55 +/- 1.5 versus 1.5 +/- 0.40, P <0.001. The results of this study strongly suggest that exposure to carbon monoxide and compounds present in wood smoke can cause

  14. Low Genetic Diversity Among Garlic (Allium sativum L. Accessions Detected Using Random Amplified Polymorphic DNA (RAPD Escasa Diversidad Genética entre Accesiones de Ajo (Allium sativum L. Detectada Mediante ADN Polimórfico Amplificado al Azar (RAPD

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    Mario Paredes C

    2008-03-01

    Full Text Available Garlic (Allium sativum L. is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. The objective of this study was to determine the genetic diversity of 65 garlic clones collected in Chile and introduced from different countries, by using RAPD (Random Amplified Polymorphic DNA. Fourty random primers of 10 mers generated a total of 398 bands with an 87% of polymorphism. Each primer amplified between two and 20 bands. The size of the fragments obtained fluctuated between 3200 and 369 bp. The results showed that the clones analyzed had a genetic similarity rate of 94%. In addition, 70% of them were clustered in one major group. However, in spite of that situation several clones have different agronomic characteristicsEl ajo (Allium sativum L. es una especie de propagación vegetativa, que presenta una amplia variabilidad morfológica. Los clones de esta especie tienen una adaptación específica a diferentes regiones agroclimáticas. El objetivo de este estudio fue determinar la diversidad genética existente en 65 clones de ajos colectados en Chile e introducidos desde diferentes países, utilizando RAPD (ADN Polimórfico Amplificado al Azar. Para esta evaluación se utilizaron 40 partidores de 10-mers. Los partidores generaron entre dos y 20 bandas, observándose un alto número de patrones con bandas múltiples. Los fragmentos generados difieren en su tamaño entre 3.200 y 369 pb. Los partidores generaron 398 bandas, de las cuales un 87% fueron polimórficas. El análisis estadístico realizado detectó una similitud genética alta, de un 94% entre las accesiones evaluadas, donde aproximadamente un 70% de los clones formaron un grupo homogéneo. Sin embargo, este grupo incluye clones que presentan diferentes características agronómicas

  15. VARIACIÓN TEMPORAL Y ESPACIAL EN POBLACIONES PREHISPÁNICAS DE CÓRDOBA. ANÁLISIS DE ADN ANTIGUO / Temporal and spatial variations in the pre-Hispanic population of Córdoba, Argentina

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    Rodrigo Nores

    2011-12-01

    Full Text Available Se presentan nuevas evidencias sobre la evolución de las poblaciones prehispánicas del actual territorio de Córdoba a partir del análisis de ADN mitocondrial de restos humanos de procedencia arqueológica de distintos contextos culturales, geográficos y temporales. La muestra estudiada incluyó 53 individuos de los cuales 48 pudieron ser genotipificados para alguno de los cuatro haplogrupos mitocondriales fundadores americanos. La totalidad de los restos analizados (excepto uno fueron previamente datados por AMS, presentando un rango de antigüedad de entre 4525 ± 20 y 345 ± 20 años AP. Los resultados obtenidos muestran diferencias genéticas significativas entre los grupos que habitaron las serranías y los que vivieron en la llanura extraserrana. Esta diferenciación, caracterizada por un fuerte incremento en la frecuencia del haplogrupo B en la zona serrana, habría surgido recién alrededor del 1200 AP, modificando sensiblemente el pool génico de la población antigua, que presenta un neto predominio del haplogrupo C. También se advierte para esa época un incremento en la frecuencia del haplogrupo A en la llanura, pero sin llegar a alterar substancialmente el perfil genético de la población antigua. Las altas prevalencias relativas en la llanura de los haplogrupos C y D, similares a la que presentan las poblaciones de Patagonia y Tierra del Fuego, coinciden, de manera sugestiva y ciertamente difícil de desentrañar, en la población contemporánea que habita la provincia de Córdoba, y sugieren un posible origen común con los habitantes del extremo sur del continente.   Palabras clave: ADN antiguo, haplogrupos mitocondriales, flujo génico, Sierras Centrales, variación regional   Abstract We present new evidence on the evolution of the populations that inhabited the territory of Cordoba in pre Hispanic times, through the analysis of mitochondrial DNA of human remains from different archaeological, geographical, and

  16. ADN bacteriano en pacientes con cirrosis y ascitis estéril: Papel como marcador de translocación bacteriana y herramienta pronóstica Bacterial DNA in patients with cirrhosis ans sterile ascites: Its role as a marker of bacterial translocation and prognosis tool

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    J. M. González-Navajas

    2007-10-01

    Full Text Available Durante la última década hemos presenciado un aumento de la cantidad de datos relativos a la presencia de translocación bacteriana en los modelos experimentales de cirrosis. Sin embargo, los estudios clínicos se han visto limitados por la falta de métodos no invasivos para estudiar dicho fenómeno. En los últimos años, las investigaciones realizadas en nuestro laboratorio se han centrado en la detección del ADN bacteriano en el suero y el líquido ascítico de los pacientes con cirrosis y ascitis estéril, y en las implicaciones clínicas que ello conlleva. Al principio, gracias a un método basado en la reacción en cadena de la polimerasa (PCR y el secuenciamiento automatizado de nucleótidos, pudimos detectar e identificar la presencia de fragmentos de ADN bacteriano en dichos pacientes con ascitis no neutrocítica y con cultivo negativo. Desde entonces hemos acumulado una serie de datos que indican que la presencia de ADN bacteriano podría desempeñar un papel importante no sólo como marcador de translocación bacteriana, sino también como factor pronóstico a corto plazo. Expondremos aquí el pasado, el presente y el futuro de esta línea de investigación.During the last decade, we have witnessed an increase in the amount of data related with the presence of bacterial translocation in experimental models of cirrhosis. However, clinical studies have been limited by the lack of non-invasive methods to study this phenomenon. Over the past years, the research developed in our laboratory has been focused on the detection of bacterial DNA in serum and ascitic fluid of patients with cirrhosis and sterile ascites, the clinical and immunological implications of such finding. Initially, by means of a polymerase chain reaction (PCR-based method and automated nucleotide sequencing, we were able to detect and identify the presence of fragments of bacterial DNA in the mentioned patients with culture-negative, non-neutrocytic ascites. Since

  17. Class Size and Cost in ADN Programs

    Science.gov (United States)

    Boehret, Alice C.; Larowe, Ann

    1978-01-01

    The article discusses the cost-effective use of faculty and facilities in associate degree nursing programs, as a large number of programs with small classes is uneconomical. Appraisal of admissions to reach an enrollment level sufficient to justify program cost is suggested. (MF)

  18. Nanomémoires adressables en ADN ?

    Science.gov (United States)

    Isambert, Hervé

    2002-04-01

    Short single-stranded DNA (or RNA) molecules can be designed to have several long lived (> hours) secondary structures. In principle, such molecules could be used as nanomemories if they could be easily induced to switch between trapped states. We propose here that the necessary work required to drive the molecule into one particular trapped state can be provided by its own synthesis. Following this idea, we argue that a low voltage (<1 V) may induce a bistable DNA molecule to switch structure at will, by forcing it to thread through a nanopore and refold alternatively from either of its ends. To cite this article: H. Isambert, C. R. Physique 3 (2002) 391-396.

  19. Reading adn Auditory-Visual Equivalences

    Science.gov (United States)

    Sidman, Murray

    1971-01-01

    A retarded boy, unable to read orally or with comprehension, was taught to match spoken to printed words and was then capable of reading comprehension (matching printed words to picture) and oral reading (naming printed words aloud), demonstrating that certain learned auditory-visual equivalences are sufficient prerequisites for reading…

  20. El premio nobel alrededor del ADN

    OpenAIRE

    Delgadillo-Álvarez, Dulce María; Instituto Politécnico Nacional (IPN)

    2016-01-01

    El Premio Nobel es un galardón internacional otorgado cada año a personas o instituciones que hayan realizado investigaciones, descubrimientos o contribuciones a la humanidad en el año inmediato anterior o en el transcurso de su vida. Los premios se instituyeron en 1895 como última voluntad del químico sueco Alfred Nobel y comenzaron a entregarse en 1901. Dos de las especialidades en las que el Premio es otorgado son en Química y en Fisiología o Medicina. El objetivo de esta breve revisión es...

  1. La baja utilidad de la determinación del ADN del VPH en la región distal de la uretra masculina Low usefulness of HPV DNA determination in the distal region of the male urethra

    Directory of Open Access Journals (Sweden)

    Ahideé G Leyva-López

    2003-01-01

    Full Text Available OBJETIVO: Determinar la prevalencia uretral del ácido desoxirribonucleico del virus de papiloma humano, condilomatosis clínica y subclínica, en hombres cuyas parejas sexuales tuvieron el antecedente de neoplasia intraepitelial cervical. MATERIAL Y MÉTODOS: De octubre de 1997 a agosto de 1998 se hizo un estudio transversal; se incluyeron 200 hombres de entre 17 a 64 años de edad, referidos a la Coordinación de Oncología del Instituto Nacional de Perinatología, de la Ciudad de México, porque sus parejas regulares sexuales tuvieron el antecedente de neoplasia intraepitelial cervical. Se llevó a cabo un examen físico del pene (penoscopía con la aplicación de ácido acético a 3-5%, y con el uso de un colposcopio se localizaron y evaluaron zonas acetoblancas y cambios vasculares, interpretados como anormales, asociados con la infección por el virus del papiloma humano. La determinación del ADN de VPH se verificó por PCR e hibridación en línea reversa. El análisis estadístico exploratorio y univariante se realizó con el paquete Stata V6.0. RESULTADOS: En las 200 muestras recolectadas de células exfoliadas de la uretra el gen de beta-globina estuvo presente en 93.5% (187/200, y el ácido desoxirribonucleico del virus del papiloma humano fue detectable solamente en 2% (4/187 de los sujetos. Por medio de la penoscopía se observó la presencia de zonas acetoblancas en 43% (81/187 de los sujetos. CONCLUSIONES: En este estudio se observa que la presencia del ácido desoxirribonucleico del virus del papiloma humano en la uretra masculina es poco común, como lo reportan estudios internacionales. Es necesario realizar investigaciones que evalúen esta presencia en glande y surco balano prepucial, en comparación con la región distal de la uretra.OBJECTIVE: To assess the prevalence of Human Papillomavirus (HPV Deoxyribonucleic acid (DNA, and of clinical and subclinical condilomatosis in men whose sex partners had been diagnosed with

  2. Uso del Fingerprinting de ADN para asignar paternidad en un rebaño con casos de malformación congénita de la pared abdominal Application of DNA Fingerprinting to determine paternity in cattle with large congenital abdominal wall defect progeny

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    N. GORLA

    1998-01-01

    Full Text Available Se evaluó la efectividad del fingerprinting de ADN para determinarla paternidad en un rebaño bovino con casos de malformacióncongénita de la pared abdominal. La técnica elegida fue elfingerprinting multilocus con la sonda (CAC5. Las muestrasde ADN fueron obtenidas de sangre periférica por los métodoshabituales, digeridas con la enzima de restricción Hae III, transferidasa membranas de nylon e hibridadas. Se analizaron en forma visual los patronesde banda obtenidos y los datos fueron procesados por el programa "PATER".A pesar de la alta consanguinidad de las razas estudiadas, la probabilidadde paternidad (W obtenida fue en un caso W = 0.80 y en el otro W = 0.93,lo que demostró la efectividad de la sonda (CAC5 paradeterminar la paternidad de un mismo toro sobre los dos terneros afectadosThe efficiency of DNA fingerprinting to solve a paternity dispute wasevaluated in four Hereford bulls of a Cebú/Hereford cattle herdwith two calves affected by a congenital abdominal wall malformation. Thetest was carried out using multi-locus probe (CAC5. 8 DNA samples ?twofrom the affected calves, two from their corresponding mothers and 4 frombulls (supposed sires? were processed. These samples were digested withrestriction enzyme Hae III, blotted onto nylon membranes and hybridisedwith (CAC5. The bands obtained were visually analysed and data was processedby the computer program "PATER" (with the "PATER" computer program. Theprobability of paternity (W was W = 0.80 in one case and W = 0.93 in theother. Despite the known inbreeding of the bovine breed tested, it waspossible to ascertain the paternity with multilocus DNA fingerprinting(CAC5

  3. La technologie ADN dans la justice pénale : une illustration de la recomposition de l’action de la justice par la science, la technique et l’expertise ? The DNA Technology in Criminal Justice: An Example of how the Interweaving of Science, Technology and Expertise Impacts on Legal Proceedings.

    Directory of Open Access Journals (Sweden)

    Bertrand Renard

    2011-10-01

    Full Text Available Le recours à l’ADN dans le processus judiciaire pénal s’est progressivement développé pour devenir en moins de 20 ans l’une des ressources scientifiques les moins contestées de la justice pénale. Cette forme d’autorité acquise en un temps assez court invite à se pencher sur les conditions sociales de l’émergence de l’ADN et de son utilisation comme élément de preuve en contexte judiciaire. Comment et dans quelle mesure l’ADN est-il endogénéisé, par le biais de l’expertise judiciaire, dans la justice et quels en sont les effets sur les raisonnements et les pratiques judiciaires ? Il convient, pour le savoir, de décrire et d’analyser les pratiques sociales relatives à l’identification par analyse génétique (IAG en justice pénale, qui sont ce par quoi l’action judiciaire intègre des éléments technologiques et les « digère ». Pour ce faire, l’article procède d’une démarche en trois temps, en illustrant d’abord l’intérêt d’ouvrir la boîte noire du travail de l’expert en IAG et en montrant ensuite à quel point l’intégration des dimensions scientifiques dans l’élaboration du droit se révèle difficile. Sur cette base enfin, l’auteur tire quelques enseignements sur les relations qu’entretiennent droit et science dans ce contexte d’un usage technologique au sein de l’action judiciaire pénale.In less than 20 years, the use of DNA has progressively increased and is now considered as one of the less problematic scientific resources in the judicial criminal process. The fact that this new form of authority has been accepted relatively quickly raises the question of the social conditions of its use as an element of proof in judicial context. How and to which extent DNA has been integrated in the legal process and has impacted on the legal reasoning and practices? What kind of role judicial experts and forensic scientists have played to translate and integrate this part of

  4. Numerical Simulation of Combustion Process in Non-toxic ADN-based Aerospace Thruster with Considering Thermal Dispersion Effect%考虑热弥散效应的ADN基无毒空间推力器内燃烧过程的数值模拟

    Institute of Scientific and Technical Information of China (English)

    张涛; 李国岫; 陈君; 虞育松; 王梦

    2016-01-01

    以新型二硝酰胺铵(ADN)基无毒空间推力器为研究对象,通过建立ADN推进剂撞网雾化、蒸发以及固定床内部考虑热弥散效应的非等温模型等,采用22种组分、20步反应的简化化学反应机理,对ADN推力器内部复杂物理化学过程进行数值模拟,并获得热弥散对ADN推力器性能的影响规律.结果表明,计算结果与实验结果具有较好的一致性.催化床的热弥散效应对ADN推进剂的催化和燃烧过程中温度,以及反应物和重要中间产物的空间分布有着重要的影响,当考虑热弥散效应时,会导致燃烧温度较高.

  5. Preparation of monodispersed Co nanoparticles and its effect on the thermal decomposition of ammonium dinitramide%单分散钴纳米粒子的制备及其对二硝酰胺铵(ADN)的热分解作用

    Institute of Scientific and Technical Information of China (English)

    段红珍; 蔺向阳; 刘冠鹏; 李凤生

    2008-01-01

    采用氢电弧等离子体法在惰性气氛中制备了单分散的高纯度纳米Co粉.利用透射电子显微镜(TEM)和相应选区电子衍射(SAED)、比表面吸附仪、X射线衍射(XRD)等手段对样品的粒度、形貌、比表面积、成分进行了表征.将纳米Co粉按不同比例与二硝酰胺铵(ADN)制成复合粒子,并用差热分析(DTA)考察了不同比例的纳米Co粉对ADN的热分解催化作用.结果表明,在ADN中加入纳米Co粉,可使ADN的分解峰温明显降低,比例越大,分解峰温的降低也越多,且表现分解热显著增大,说明纳米Co粉对ADN有较强的催化作用.

  6. Research trends in radiobiology since 40 years. a new approach: the enzymatic repair function of DNA, internal factor in evolution of biological systems under irradiation; Etude des tendances des recherches en radiologie depuis 40 ans. Une nouvelle voie de recherche: la fonction de reparation enzymatique de l'ADN, facteur interne d'evolution des systemes biologiques sous rayonnement

    Energy Technology Data Exchange (ETDEWEB)

    Mouton, R. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1968-07-01

    . Il y a moins d'une generation, l'on croyait encore que les effets des rayonnements sur les systemes vivants etaient des effets directs, immediats, irreparables et definitifs. Depuis lors, on s'est rendu compte que les radiolesions pouvaient resulter d'effets indirects, etre tardives, reparables, et le plus souvent modifiables par des traitements chimiques ou biochimiques ad hoc. Cependant, la percee decisive en radiobiologie se situe entre 1962 et 1964, avec la decouverte que la cellule possede un mecanisme de defense active vis-a-vis de tout ce qui peut porter atteinte a l'integrite du patrimoine genetique inscrit dans la structure meme de l'ADN. L'existence d'une quatrieme fonction de l'ADN, celle d'auto-reparation, par voie enzymatique sous controle genetique, apporte enfin a la radiobiologie la base biologique moleculaire qui lui manquait pour sortir de la nuit 'phenomenologique' ou elle s'etait enfoncee apres l'abandon de la generalisation de la theorie de Lea, faute de preuve experimentale. Dans la seconde partie, cette fois prospective, de l'etude l'auteur tente une synthese elargie en considerant le role que peut jouer cette fonction de reparation de l'ADN non seulement du point de vue de la survie des systemes biologiques irradies en presence ou non d'agents modificateurs de dose ou mutagenes, mais aussi de celui de l'evolution naturelle ou artificielle de ces systemes sous rayonnement. Les donnees les plus recentes de la litterature sont en accord avec ce qui ne constitue encore qu'une hypothese generale de recherche. Des travaux portant sur les caracteres phenotypiques et genotypiques lies a l'acquisition de radioresistance gamma et UV, chez 'Escherichia coli K12', ont ete entrepris par l'auteur, en collaboration avec O. Tremeau en vue d'apporter une contribution experimentale nouvelle a cet egard. (auteur)

  7. Inmunofluorescencia con Crithidia luciliae para la detección de anticuerpos anti-ADN: Imágenes atípicas y su relación con enfermedad de Chagas y leishmaniasis Immunofluorescence assay with Crithidia luciliae for the detection of anti-DNA antibodies: Atypical images and their relationship with Chagas’ disease and leishmaniasis

    Directory of Open Access Journals (Sweden)

    Gloria Griemberg

    2006-02-01

    Full Text Available Los anticuerpos anti-ADN nativo pueden detectarse por inmunofluorescencia indirecta con Crithidia luciliae, observándose tinción fluorescente anular del kinetoplasto que contiene ADN de doble cadena. En algunos casos pueden observarse imágenes fluorescentes en flagelo, membrana y corpúsculo basal, consideradas atípicas. Como C. luciliae pertenece a la familia Trypanosomatidae, que incluye patógenos para el hombre como Trypanosoma cruzi y Leishmaniaspp., se consideró que las imágenes atípicas pudieran deberse a reacciones cruzadas. Se realizaron estudios serológicos para Chagas a 105 muestras provenientes de zona endémica (Corrientes y no endémica (Buenos Aires para T. cruzi que presentaban imágenes atípicas con C. luciliae. La serología para Chagas resultó positiva en el 64.7% de las muestras de Buenos Aires y en el 78.3% de las de Corrientes que presentaban frente a C. luciliae imagen conjunta de membrana y flagelo. No presentaron la imagen conjunta ninguna de las muestras de dadores de sangre normales, ni de pacientes con enfermedades del tejido conectivo, excepto dos con lupus que también eran chagásicos. Todas las muestras de pacientes chagásicos analizadas frente a C. luciliae presentaron la imagen conjunta. Se estudiaron también 46 muestras de pacientes con leishmaniasis, 28 de ellos coinfectados con T. cruzi. La imagen conjunta se observó en el 88.0% de las muestras de leishmaniásicos y en el 78.5% de las de coinfectados. Los resultados sugieren que C. luciliae podría ser un sustrato alternativo, económico y de bajo riesgo para el diagnóstico serológico de enfermedad de Chagas, aunque no discrimina la infección por Leishmania. El hallazgo de la imagen conjunta en la detección de anti-ADN nativo señala la conveniencia de realizar en esos pacientes, estudios clínicos y de laboratorio para enfermedad de Chagas y leishmaniasis.Anti-native DNA antibodies can be detected by indirect immunofluorescence assay with

  8. Enfermedades genéticas del ADN mitocondrial humano

    Directory of Open Access Journals (Sweden)

    Solano Abelardo

    2001-01-01

    Full Text Available Las enfermedades mitocondriales son un grupo de trastornos que están producidos por un fallo en el sistema de fosforilación oxidativa (sistema Oxphos, la ruta final del metabolismo energético mitocondrial, con la consiguiente deficiencia en la biosíntesis del trifosfato de adenosina (ATP, por sus siglas en inglés. Parte de los polipéptidos que componen este sistema están codificados en el ácido desoxirribonucleico (DNA mitocondrial y, en los últimos años, se han descrito mutaciones que se han asociado con síndromes clínicos bien definidos. Las características genéticas del DNA mitocondrial, herencia materna, poliplasmia y segregación mitótica, confieren a estas enfermedades propiedades muy particulares. Las manifestaciones clínicas de estas enfermedades son muy heterogéneas y afectan a distintos órganos y tejidos por lo que su correcto diagnóstico implica la obtención de datos clínicos, morfológicos, bioquímicos y genéticos. El texto completo en inglés de este artículo está disponible en: http://www.insp.mx/salud/index.html

  9. Inhibition of Human Arginase I by Substrate adn Product Analogues

    Energy Technology Data Exchange (ETDEWEB)

    L Di Costanzo; M Ilies; K Thorn; D Christianson

    2011-12-31

    Human arginase I is a binuclear manganese metalloenzyme that catalyzes the hydrolysis of L-arginine to generate L-ornithine and urea. We demonstrate that N-hydroxy-L-arginine (NOHA) binds to this enzyme with K(d)=3.6 microM, and nor-N-hydroxy-L-arginine (nor-NOHA) binds with K(d)=517 nM (surface plasmon resonance) or K(d) approximately 50 nM (isothermal titration calorimetry). Crystals of human arginase I complexed with NOHA and nor-NOHA afford 2.04 and 1.55 A resolution structures, respectively, which are significantly improved in comparison with previously-determined structures of the corresponding complexes with rat arginase I. Higher resolution structures clarify the binding interactions of the inhibitors. Finally, the crystal structure of the complex with L-lysine (K(d)=13 microM) is reported at 1.90 A resolution. This structure confirms the importance of hydrogen bond interactions with inhibitor alpha-carboxylate and alpha-amino groups as key specificity determinants of amino acid recognition in the arginase active site.

  10. ADN Programs Accredited by the National League for Nursing, 1974

    Science.gov (United States)

    Nursing Outlook, 1974

    1974-01-01

    The complete list of programs leading to an associate degree in nursing that are accredited by the National League for Nursing is presented, without annotation. The institutions are listed alphabetically by State. (Author/AJ)

  11. Differences in Clinical Experiences of ADN and BSN Students.

    Science.gov (United States)

    Oermann, Marilyn H.

    1998-01-01

    In a study of 211 associate degree (AD) and 204 baccalaureate nursing students, AD students reported significantly higher stress in clinical practice. Stress for both groups increased as they progressed. Instructors were the predominant source of stress. Students had the most difficulty coping with the demands of patient care and the clinical…

  12. CHARACTERIZATION ADN BIOLOGICAL ACTIVITY OF SECONDARY METABOLITES FROM ARMILLARIA TABESCENS

    Science.gov (United States)

    Ethyl acetate extracts from liquid cultures of Armillaria tabescens showed good antimicrobial activity against Candida albicans, Cryptococcus neoformans, Escherichia coli and Mycobacterium intracellulare. Chemical analyses of extract constituents led to the isolation and identification of two new co...

  13. The genus Basilichthys (Teleostei: Atherinopsidae revisited along its Chilean distribution range (21° to 40° S using variation in morphologyand mtDNA El género Basilichthys (Teleostei: Atherinopsidae analizado a lo largo de su distribución en Chile (21° a 40° S, utilizando rasgos morfológicos y variabilidad del ADN mitocondrial

    Directory of Open Access Journals (Sweden)

    DAVID VÉLIZ

    2012-03-01

    peligro de extinción es el de los pejerreyes del género Basilichthys. A lo largo Chile, este género posee tres especies morfológicas con distribución disjunta: Basilichthys semotilus, B. microlepidotus y B. australis. Las dos últimas sobreponen su distribución en el río Aconcagua y no son fácilmente diferenciables morfológicamente. Para evaluar la eficacia en la identificación de estas especies al utilizar marcadores moleculares, se analizó el 9 % del ADN mitocondrial (Región Control y COI de organismos obtenidos desde el río Loa (21°41' S al río Valdivia (39°50' S y adicionando un análisis merístico en organismos pertenecientes a las especies B. microlepidotus y B. australis. El análisis filogenético muestra que los individuos de B. semotilus forman un haplogrupo separado de las otras especies del género, sin embargo, B. australis y B. microlepidotus serían parte de un mismo grupo monofilético. Un segundo análisis, el cual incluye información merística, no muestra diferencias estadísticas significativas en la cantidad de escamas de la línea lateral, y número de rayos en las aletas entre B. microlepidotus y B. australis. Estos resultados no sustentan la clasificación actual, separando claramente un grupo presente en el extremo norte del país (B. semotilus y un segundo grupo en Chile central el cual debería ser llamado B. microlepidotus. Esta información será importante para revisar el estado de conservación de la ictiofauna chilena.

  14. Presence of a Phytoplasma Associated with Witches’-Broom Disease in Ugni molinae Turcz. and Gaultheria phillyreifolia (Pers. Sleumer Determined by DAPI, PCR, and DNA Sequencing Presencia de un Fitoplasma Asociado a la Enfermedad de "Escoba de Bruja" en Ugni molinae Turcz. y Gaultheria phillyreifolia (Pers. Sleumer Determinado Mediante DAPI, PCR y Secuenciación de ADN

    Directory of Open Access Journals (Sweden)

    Nolberto Arismendi S

    2010-03-01

    ón que no ocurrió en plantas asintomáticas. En la verificación mediante PCR-anidada, se logró amplificar ADN fitoplasmático en plantas de murta y chaura enfermas, pero no en plantas aparentemente sanas. La secuenciación de los productos amplificados permitió localizar al fitoplasma dentro del grupo “ash yellows" (16SrVII y relacionado al “Candidatus Phytoplasma fraxini”. Éste es el primer reporte que caracteriza a un fitoplasma en especies nativas chilenas. Considerando la diversidad de especies de plantas infectadas por el grupo ash yellows, sugiere que G. phillyreifolia y U. molinae podrían constituir un reservorio de fitoplasmas para otros cultivos agrícolas de importancia económica.

  15. Études par diffraction de fibres de l'ADN double brin

    Science.gov (United States)

    Forsyth, V. T.; Parrot, I. M.

    2005-11-01

    L'état fibreux est un état naturel pour les molécules de polymère qui ont tendance à adopter des conformations hélicoïdales régulières plutôt que des structures globulaires caractéristiques à de nombreuses protéines. La diffraction de fibres a donc une application étendue pour l'étude d'une grande variété de polymères biologiques et synthétiques. Ce papier a pour objectif d'illustrer l'étendue générale de la méthode et, en particulier, de démontrer l'impact des sources modernes de rayonnement synchrotron et de faisceaux neutroniques.

  16. Proposal of a relationship between dynamic aperture adn intensity evolution in a storage ring

    CERN Document Server

    Giovannozzi, M

    2010-01-01

    A scaling law for the time-dependence of the dynamic aperture, i.e., the region of phase space where stable motion occurs, was proposed in previous papers, about ten years ago. The use of fundamental theorems of the theory of dynamical systems allowed showing that the dynamic aperture has a logarithmic dependence on time. In this paper this result, proved by mean of numerical simulations, is used as a basis for deriving a scaling law for the intensity evolution in a storage ring. The proposed scaling law is also tested against experimental data showing a remarkable agreement.

  17. [Erythropoiesis and functional characteristics in bone marrow erythroblastic islets during stimulated adn inhibited erythropoiesis].

    Science.gov (United States)

    Rassokhin, A G; Kruglov, D G; Zakharov, Iu M

    2000-01-01

    When erythropiesis is stimulated (acute blood loss) or inhibited (posttransfusion polycythemia), there are early changes in the cytochemical values of erythroblastic islets (EI): in the levels of acid and neutral glucoconjugates and in the activity of nonspecific esterase. A close correlation has been found between the erythropoiesis in EI and its functional characteristics. It is concluded that central macrophages play the key role in the modulation of EI erythropoiesis. It is suggested that EI macrophages are involved in the provision of bioenergetic and reparative processes in EI.

  18. Using an admissions exam to predict student success in an ADN program.

    Science.gov (United States)

    Gallagher, P A; Bomba, C; Crane, L R

    2001-01-01

    Nursing faculty strive to admit students who are likely to successfully complete the nursing curriculum and pass NCLEX-RN. The high cost of academic preparation and the nursing shortage make this selection process even more critical. The authors discuss how one community college nursing program examined academic achievement measures to determine how well they predicted student success. Results provided faculty with useful data to improve the success and retention of nursing.

  19. ADN-Viewer: a 3D approach for bioinformatic analyses of large DNA sequences.

    Science.gov (United States)

    Hérisson, Joan; Ferey, Nicolas; Gros, Pierre-Emmanuel; Gherbi, Rachid

    2007-01-20

    Most of biologists work on textual DNA sequences that are limited to the linear representation of DNA. In this paper, we address the potential offered by Virtual Reality for 3D modeling and immersive visualization of large genomic sequences. The representation of the 3D structure of naked DNA allows biologists to observe and analyze genomes in an interactive way at different levels. We developed a powerful software platform that provides a new point of view for sequences analysis: ADNViewer. Nevertheless, a classical eukaryotic chromosome of 40 million base pairs requires about 6 Gbytes of 3D data. In order to manage these huge amounts of data in real-time, we designed various scene management algorithms and immersive human-computer interaction for user-friendly data exploration. In addition, one bioinformatics study scenario is proposed.

  20. Microorganisms Taken to Far Extreme: an Update on Their Survival adn Growth

    Science.gov (United States)

    Sharma, A.

    2006-12-01

    The questions that make geology at extremes so fascinating involve attempts on understanding the deep subsurface processes and their effect on what we find directly relevant at near surface short time scale interactions. What is the base of the biosphere? What are the various niches life as we know can persist? These are few of the questions that are relevant to deep subsurface geology. Not unlike any other scientific inquiry, along with extensive field and theoretical studies, these geomicrobiological questions need an experiment-based evaluation that can help constrain the geochemical parameters relevant to life's survival. Sharma et al. (2002) have taken a direct approach in constraining the microbial activity at extreme conditions by making observations within diamond anvil cells. Specific chemical component (formate) was used to constrain the metabolic activity of ambient pressure microbes at high pressures. This study opened up the possibility of life in radically extreme environments, often deficient of liquid water and showed that microbial life can find niches within the organic rich veins and inclusions, such as in (dense phase) ice. High resolution imaging within the diamond cell has provided a better insight into the state of the ambient pressure microbes. The author will present newer results on microbial survival at high pressures that provide in insight into the heterogeneous effect of high hydrostatic pressure affecting some microbes differently such that they do perish, while others remain largely viable. By monitoring microbial growth upon decompression, these experiments show the viability of the microbes at high pressures and hence the feasibility of a deep biosphere. The author will also present a combination of high temperature and pressure on the microbiological system at such far extreme conditions that show some surprising behavior.

  1. Optimization adn Guidance of Very Low-Thrust Transfers to Geostatoinary Orbit

    Science.gov (United States)

    Gil-Fernandez, J.; Tarabini, L.; Graziano, M.; Gomez-Tierno, M. A.

    A new hybrid direct-indirect optimization algorithm is presented to compute the minimum-time transfer between two orbits, including the phasing with a desired spacecraft. Very-low thrust means several hundred revolutions to perform the large change in orbital elements. The optimal control solution of the fast-evolution problem combined with a direct method for the secular trajectory avoids the numerical instability arising in very long propagations, decreases the computational time, reduces the sensitivity to the initial guess and provides a feasible transfer at every optimization step. Optimization of transfers from GTO to GEO is presented and two types of trajectories are analysed, sub-synchronous (apogee constrained below GEO altitude) and super-synchronous (free apogee altitude). The optimization of a transfer from LEO to a very high orbit (11 x 23 RE) is presented, showing the applicability of the method to different problems. A guidance algorithm is presented to compensate the deviations of the real trajectory from the optimal one due to off-nominal conditions. The results in closed-loop simulation of the guidance scheme to compensate deterministic perturbations not considered in the optimization show good performances in both analysed missions.

  2. Evaluation of a Successful High Risk Nursing Student Assistance Program: One ADN Program's Journey

    Science.gov (United States)

    O'Sullivan, Ciaran Anthony Mary

    2013-01-01

    A college education is, for many in America, part and parcel of the American Dream, and is certainly achievable. For countless reasons, students may enroll at community colleges underprepared, unprepared, anxious, and destined for a high risk of failure. Although community colleges are higher education institutions open and accessible to all who…

  3. Predictors of Success and Failure for ADN Students on the NCLEX-RN

    Science.gov (United States)

    Benefiel, Diane

    2011-01-01

    The purpose of this study was to: 1) analyze the relationship of preprogram and nursing program variables on National Council Licensure Examination for Registered Nurses (NCLEX-RN) success and failure, and 2) develop a model to predict success and failure on the NCLEX-RN. The convenience sample was comprised of 245 spring, summer, and fall midterm…

  4. SRTM mission-cross comparison of X adn C band data properties

    Science.gov (United States)

    Rosen, P.; Eineder, M.; Rabus, B.; Gurrola, E.; Hensley, S.; Knopfle, W.; Breit, H.; Roth, A.; Werner, M.

    2001-01-01

    This paper compares the specific properties of the X and C band data sets with respect to global coverage, height accuracy, sensor specific errors, product definition, product format and availability.

  5. Student and Faculty Perceptions of Effective Clinical Instructors in ADN Programs.

    Science.gov (United States)

    Gignac-Caille; Anne Marie; Oermann, Marilyn H.

    2001-01-01

    Associate degree nursing students (n=292) and faculty (n=59) agreed on 6 of 10 characteristics of effective clinical instructors. Effective instructors are clinically competent, use effective evaluation strategies, explain clearly, are approachable, are well prepared to teach, and communicate clear expectations. (Contains 28 references.) (SK)

  6. ESTUDIO TEORICO DE TRANSFERENCIAS PROTONICAS EN BASES DE ADN Y EN SISTEMAS MODELO.

    OpenAIRE

    HERRERA PISANI, BARBARA ANDREA

    2005-01-01

    Uno de los objetivos principales de este trabajo de Tesis ha sido caracterizar reacciones de transferencia protónica en sistemas modelo, tales como, el ácido nitrosos y la serie de sus derivados azufrados HXNY (X,Y=O,S) y en sistema de interés biológico c 133p.

  7. Enfermedades genéticas del ADN mitocondrial humano Genetic diseases of the mitochondrial DNA

    Directory of Open Access Journals (Sweden)

    Abelardo Solano

    2001-04-01

    Full Text Available Las enfermedades mitocondriales son un grupo de trastornos que están producidos por un fallo en el sistema de fosforilación oxidativa (sistema Oxphos, la ruta final del metabolismo energético mitocondrial, con la consiguiente deficiencia en la biosíntesis del trifosfato de adenosina (ATP, por sus siglas en inglés. Parte de los polipéptidos que componen este sistema están codificados en el ácido desoxirribonucleico (DNA mitocondrial y, en los últimos años, se han descrito mutaciones que se han asociado con síndromes clínicos bien definidos. Las características genéticas del DNA mitocondrial, herencia materna, poliplasmia y segregación mitótica, confieren a estas enfermedades propiedades muy particulares. Las manifestaciones clínicas de estas enfermedades son muy heterogéneas y afectan a distintos órganos y tejidos por lo que su correcto diagnóstico implica la obtención de datos clínicos, morfológicos, bioquímicos y genéticos. El texto completo en inglés de este artículo está disponible en: http://www.insp.mx/salud/index.htmlMitochondrial diseases are a group of disorders produced by defects in the oxidative phosphorylation system (Oxphos system, the final pathway of the mitochondrial energetic metabolism, resulting in a deficiency of the biosynthesis of ATP. Part of the polypeptide subunits involved in the Oxphos system are codified by the mitochondrial DNA. In the last years, mutations in this genetic system have been described and associated to well defined clinical syndromes. The clinical features of these disorders are very heterogeneous affecting, in most cases, to different organs and tissues and their correct diagnosis require precise clinical, morphological, biochemical and genetic data. The peculiar genetic characteristics of the mitochondrial DNA (maternal inheritance, polyplasmia and mitotic segregation give to these disorders very distinctive properties. The English version of this paper is available at: http://www.insp.mx/salud/index.html

  8. Vacunas de ADN: inducción de la respuesta inmunitaria

    OpenAIRE

    Javier Mota-Sánchez

    2009-01-01

    La efectividad de las vacunas y la inmunización en la prevención de las enfermedades infecciosas es uno de los grandes avances de la medicina. En la actualidad, el acceso a la tecnología de punta en el área de la genómica y la proteómica ha hecho posible acelerar el desarrollo de nuevos modelos de vacunas con características mejoradas en aspectos fundamentales, como la inmunogenicidad y la seguridad. A casi dos décadas del primer informe, en el cual se demostró que un gen puede expresarse med...

  9. The Lewis Acid-promoted Novel Cyclization Reactions Towards N-adn O-Containing Heterocycles

    Institute of Scientific and Technical Information of China (English)

    Shoko; Yamazaki

    2007-01-01

    1 Results Nitrogen and oxygen-containing heterocyclic systems are important structures in organic chemistry because of their presence in many biologically active compounds.In this work,a new zinc and indium-promoted conjugate addition-cyclization reaction to afford nitrogen and oxygen-containing five-membered heterocycles has been developed.A Lewis acid-catalyzed cyclization of an ethenetricarboxylate derivative with propargylamines or propargyl alcohols to give methylenepyrrolidines and methylenetetrah...

  10. ESPERMIOGRAMAS DIGITALES ASISTEIDOS POR INTERNET (ED*AI): CONCENTRACION, MOVILIDAD, VITALIDAD, MORFOLOGIA Y FRAGMENTACION ADN ESPERMATICO

    OpenAIRE

    HARTEL GRUNDLER, STEFFEN

    2012-01-01

    La infertilidad afecta alrededor del 15% de las parejas y constituye un creciente desafío de la salud pública mundial. El espermiograma permite definir la severidad del factor masculino y constituye un antecedente fundamental en el diagnóstico y determinación de las posibles estrategias de tratamiento clínico. Durante 2009-2012, el proyecto Espermiogramas Digitales Asistidos por Internet (ED•AI) apuntó al desarrollo de un nuevo servicio tele analítico | tele médico que garantiza e...

  11. Efecto del tabaco sobre la calidad seminal: Estudio citogenético y molecular del ADN

    OpenAIRE

    Viloria Samochin, Thamara Alexandra

    2008-01-01

    A lo largo de los años, muchos han sido los factores relacionados con la infertilidad masculina, como: infecciones, varicocele (Pomerol 2002), obstrucciones y alteraciones en la producción espermática (Ballescá 2002), causas genéticas (Egozcue 2002), entre otras. Muchas veces la causa es desconocida y pueden afectar en mayor o menor medida a la calidad seminal. Sin embargo, su relación con los hábitos tóxicos ha sido motivo de investigación en los últimos años; de sus resultados se han descri...

  12. Correlates of Ability Patterns in Children adn Adults: Implications for Teaching

    Science.gov (United States)

    Barton, Keith; Horowitz, Alan B.

    1975-01-01

    Presents research evidence that children with a high verbal-low spatial ability profile tend to respond in the "usual" way to social reinforcement or praise, whereas children with a high spatial-low verbal profile do not find praise reinforcing. Implications for teaching considered in detail. (Author)

  13. Ttheek'adn Ut'iin Yaaniida Qqnign' = Old Time Stories of the Scottie Creek People.

    Science.gov (United States)

    Tyone, Mary; Kari, James, Ed.

    This collection of 18 stories of the Scottie Creek People of Alaska is presented in both Upper Tanana, an Athabaskan language, and English in line-by-line translation. The stories, which include personal narratives, cultural explanations, and traditional tales, were recorded in 1988-94. The tales are told by Mary Tyone, a traditional storyteller…

  14. Frenando la extinción: utilidad del ADN en especies en peligro

    OpenAIRE

    Fernández-Alonso, M. Carmen; Platón, María

    2016-01-01

    Cartel anunciador de la charla; Presentación del ciclo de charlas “Jam Science-Divulgación Científica” celebradas en el Moe Club (Alberto Alcocer, 32, Madrid) el penúltimo martes de cada mes de 20.00 a 22.00h, desde enero de 2015.Ponente:Dr. Jose Luis Hórreo.Investigador posdoctoral, en el Museo Nacional de Ciencias Naturales (MNCN-CSIC). Grabación y edición de los vídeos: Agora Mundi.

  15. Relación entre los haplogrupos del ADN mitocondrial y la sordera por cisplatino

    OpenAIRE

    Graterol Torres, Domingo Jesús

    2015-01-01

    ·Introducción El cisplatino es un quimioterápico muy eficaz, empleado en múltiples neoplasias de diversos tipos. Sin embargo causa importantes efectos adversos en los pacientes que lo reciben, como por ejemplo: nefrotoxicidad, neurotoxicidad y ototoxicidad. Aproximadamente entre un 20 y 40 % de los pacientes que reciben este fármaco desarrollan ototoxicidad la cual se manifiesta generalmente como pérdida auditiva bilateral, simétrica, irreversible y en frecuencias agudas. Los factores de ries...

  16. Relación entre los haplogrupos del ADN mitocondrial y la sordera por cisplatino

    OpenAIRE

    Graterol Torres, Domingo Jesús

    2015-01-01

    • Introducción El cisplatino es un quimioterápico muy eficaz, empleado en múltiples neoplasias de diversos tipos. Sin embargo causa importantes efectos adversos en los pacientes que lo reciben, como por ejemplo: nefrotoxicidad, neurotoxicidad y ototoxicidad. Aproximadamente entre un 20 y 40 % de los pacientes que reciben este fármaco desarrollan ototoxicidad la cual se manifiesta generalmente como pérdida auditiva bilateral, simétrica, irreversible y en frecuencias agudas. Los factores...

  17. Síntesis de sustratos del surco menor del ADN con estructura tipo glico-oligoamida

    OpenAIRE

    2011-01-01

    En la port.: Instituto de Química Orgánica General (CSIC) Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Química Orgánica. Fecha de lectura: 27/1/2011

  18. En búsqueda del ADN y las influencias en algunos sistemas presidenciales y parlamentarios

    OpenAIRE

    Jorge CARPIZO

    2009-01-01

    El trabajo examina las recíprocas influencias entre las Constituciones de seis países: tres con sistema presidencial, dos con sistema parlamentario y uno con sistema semipresidencial. Los tres últimos pertenecen a Europa. Los sistemas presidenciales de América Latina se inspiraron en la Constitución Americana, tanto como en alguna europea, especialmente la española de 1812. Los enormes poderes presidenciales en América Latina llevaron a introducir en el sistema preside...

  19. Adopción e identidad: ¿ADN sin historia?

    OpenAIRE

    2009-01-01

    El trabajo a presentar es producto de la investigación realizada en mi tesis de Maestría en Derechos Humanos, cuyo tema principal es “El derecho a la identidad en niños, niñas y adolescentes adoptados/as en Argentina”, temática que continúo profundizando como becaria del CIC en lo concerniente al modo formulación y aplicación de la Ley 13.298. Parto de una perspectiva interdisciplinaria que aúna el enfoque de derechos humanos y psicológico, con el fin de poder profundizar sobre qué aportes br...

  20. Efecto de la suplementación de metionina sobre el estrés oxidativo y de la edad sobre la presencia de ADN mitocondrial en el ADN nuclear, en rata Wistar

    OpenAIRE

    Gómez Sánchez, José

    2010-01-01

    La restricción de un único aminoácido en dieta, la metionina, puede incrementar la longevidad máxima, retrasar la aparición de enfermedades asociadas al envejecimiento y reducir tanto la producción mitocondrial de radicales libres de oxígeno como el estrés oxidativo. La suplementación de metionina genera una gran toxicidad y causa daño en diferentes órganos, sin embargo no está claro el mecanismo por el cual se generan los efectos adversos. Además se desconoce si la suplementación de metionin...

  1. Observation des cycles enzymatiques des ADN topoisomérases par micromanipulation de molécules individuelles

    Science.gov (United States)

    Strick, Terence R.; Charvin, Gilles; Dekker, Nynke H.; Allemand, Jean-François; Bensimon, David; Croquette, Vincent

    In this article, we describe single-molecule assays using magnetic traps and we applied these assays to topoisomerase enzymes which unwind and disentangle DNA molecules. First, the elasticity of single DNA molecule is characterized using the magnetic trap. We show that a twisting constraint may be easily applied and that its effect upon DNA may be measured accurately. Then we describe how the topoisomerase activity may be observed at the single-molecule level giving direct access to the important biological parameters of the enzyme such as velocity and processivity. Furthermore, individual cycles of unwinding can be observed in real time. This permits an accurate characterization of the enzyme's biochemical cycle. The data treatment required to identify and analyze individual topoisomerization cycles will be presented in detail. This analysis is applicable to a wide variety of molecular motors. To cite this article: T.R. Strick et al., C. R. Physique 3 (2002) 595-618.

  2. [Regional anaesthesia for labor adn delivery in a parturient with neuropathy with liability to pressure palsy (tomaculous neuropathy)].

    Science.gov (United States)

    Berdai, S; Benhamou, D

    2004-10-01

    Tomaculous neuropathy (or hereditary neuropathy with liability to pressure palsy [HNLPP]) is a rare and hereditary disease which incidence has probably been underestimated. It is characterised by demyelination resulting in numbness and weakness after nerve pressure, injury or stretch. Despite a well-documented genetic pathophysiologic mechanism, implications for anaesthesia in patients with HNLPP are only speculative and the use of regional anaesthesia is debatable. We report here the case of a patient with HNLPP who was followed during two consecutive pregnancies in the same hospital and for whom an expert of the SOS-RA hotline service was consulted before each delivery. For the first delivery, epidural analgesia was performed for labour pain control but a caesarean section was necessary because of failure to progress (0.0625% bupivacaine with 0.2 microg/ml sufentanil for labour then 2% lidocaine with adrenaline for surgery). Two years later, the patient was again seen for a preanaesthetic visit because elective Caesarean section was planned. Spinal anaesthesia using hyperbaric bupivacaine and sufentanil was used. Both deliveries were uneventful and there were no neurologic complaints in the postpartum periods.

  3. Código de barras del ADN y sus posibles aplicaciones en el campo de la Entomología

    Directory of Open Access Journals (Sweden)

    Analía A. LANTERI

    2007-01-01

    Full Text Available En este artículo se abordan algunos aspectos de la controversia sobre la iniciativa «Código de barras del ADN», y se hace hincapié en sus potenciales aplicaciones en Entomología. Esta iniciativa propone emplear información dentro de una misma región génica (gen mitocondrial de la Citocromo c Oxidasa I = COI, en todas las especies vivientes y con condiciones de secuenciación universalmente aceptadas y estandarizadas. En la actualidad, no pretende sustituir la taxonomía alfa y la filogenia sino agilizar las tareas de identificación, especialmente en el campo de la Biomedicina (identificación de patógenos, parásitos y vectores, el control de plagas (intercepción de especies invasoras, cualquiera sea su estado de desarrollo ontogenético y los estudios sobre conservación de la biodiversidad. Para arribar a una correcta delimitación de las especies biológicas es preciso contar con las secuencias de COI de numerosos individuos a lo largo de todo su rango geográfico y además, secuencias de genes nucleares e información morfológica y biológica detallada. Las «Unidades Evolutivas Significativas», identificadas sobre la base del «código de barras», podrían corresponder tanto a morfoespecies como a especies crípticas y a subespecies o linajes con diferentes preferencias de huéspedes. La integración del «código de barras del ADN», el trabajo de campo, las colecciones de museos y la investigación científica resultan imprescindibles para que esta herramienta redunde en avances significativos en el campo de la Sistemática Entomológica.

  4. Effects of ionizing radiations on DNA-protein complexes; Effets des radiations ionisantes sur des complexes ADN-proteine

    Energy Technology Data Exchange (ETDEWEB)

    Gillard, N

    2005-11-15

    The radio-induced destruction of DNA-protein complexes may have serious consequences for systems implicated in important cellular functions. The first system which has been studied is the lactose operon system, that regulates gene expression in Escherichia coli. First of all, the repressor-operator complex is destroyed after irradiation of the complex or of the protein alone. The damaging of the domain of repressor binding to DNA (headpiece) has been demonstrated and studied from the point of view of peptide chain integrity, conformation and amino acids damages. Secondly, dysfunctions of the in vitro induction of an irradiated repressor-unirradiated DNA complex have been observed. These perturbations, due to a decrease of the number of inducer binding sites, are correlated to the damaging of tryptophan residues. Moreover, the inducer protects the repressor when they are irradiated together, both by acting as a scavenger in the bulk, and by the masking of its binding site on the protein. The second studied system is formed by Fpg (for Formamido pyrimidine glycosylase), a DNA repair protein and a DNA with an oxidative lesion. The results show that irradiation disturbs the repair both by decreasing its efficiency of DNA lesion recognition and binding, and by altering its enzymatic activity. (author)

  5. Final Technical Report - High-Performance, Oxide-Dispersion-Strengthened Tubes for Production of Ethylene adn Other Industrial Chemicals

    Energy Technology Data Exchange (ETDEWEB)

    McKimpson, Marvin G.

    2006-04-06

    This project was undertaken by Michigan Technological University and Special Metals Corporation to develop creep-resistant, coking-resistant oxide-dispersion-strengthened (ODS) tubes for use in industrial-scale ethylene pyrolysis and steam methane reforming operations. Ethylene pyrolysis tubes are exposed to some of the most severe service conditions for metallic materials found anywhere in the chemical process industries, including elevated temperatures, oxidizing atmospheres and high carbon potentials. During service, hard deposits of carbon (coke) build up on the inner wall of the tube, reducing heat transfer and restricting the flow of the hydrocarbon feedstocks. About every 20 to 60 days, the reactor must be taken off-line and decoked by burning out the accumulated carbon. This decoking costs on the order of $9 million per year per ethylene plant, accelerates tube degradation, and requires that tubes be replaced about every 5 years. The technology developed under this program seeks to reduce the energy and economic cost of coking by creating novel bimetallic tubes offering a combination of improved coking resistance, creep resistance and fabricability not available in current single-alloy tubes. The inner core of this tube consists of Incoloy(R) MA956, a commercial ferritic Fe-Cr-Al alloy offering a 50% reduction in coke buildup combined with improved carburization resistance. The outer sheath consists of a new material - oxide dispersion strengthened (ODS) Alloy 803(R) developed under the program. This new alloy retains the good fireside environmental resistance of Alloy 803, a commercial wrought alloy currently used for ethylene production, and provides an austenitic casing to alleviate the inherently-limited fabricability of the ferritic Incoloy(R) MA956 core. To provide mechanical compatibility between the two alloys and maximize creep resistance of the bimetallic tube, both the inner Incoloy(R) MA956 and the outer ODS Alloy 803 are oxide dispersion strengthened materials produced using mechanical alloying technology. To minimize cost, the bimetallic tube is produced by direct powder co-extrusion. This technology has potential for domestic energy savings of up to 4.1 trillion BTU/year (4.3 x 1015J/year) and a reduction of 370,000 tons (340,000 tonnes) of CO2 emissions in short-residence-time ethylene furnaces. This represents an energy savings and CO2 emissions reduction of about 3.3%. If the technology is also applied to other types of ethylene pyrolysis furnaces, total energy savings and CO2 emissions reductions could increase by up to five times. The work involved: Developing powder and consolidation processing protocols to produce an oxide-dispersion strengthened variant of Alloy 803 exhibiting creep strength comparable to Incoloy? Alloy MA956, Developing a direct powder co-extrusion protocol for fabricating co-extruded bimetallic Incoloy? Alloy MA956 / ODS Alloy 803 tubes, Characterizing the properties of the ODS Alloy 803 material, the welding characteristics of the bimetallic tubes, and the coking characteristics of the Incoloy? MA956 alloy, and Documenting the potential energy savings and user requirements for these bimetallic pyrolysis furnace tubes. The project demonstrated that oxide dispersion strengthened Alloy 803 can be produced successfully using conventional mechanical alloying technology. The oxide dispersion strengthened bimetallic radiant coil technology explored under this program has significant potential for energy savings and productivity improvements for domestic ethylene producers. In today's competitive market, however, domestic furnace manufacturers and ethylene producers appear reluctant to pay any cost premium for higher-performance coil materials offering either higher temperature capabilities or longer service life. Interest in oxide dispersion strengthened radiant coils is likely to increase if furnace and ethylene producers begin to focus more on increasing tube wall temperatures to improve productivity.

  6. The importance of hydraulic groundwater theory in catchment hydrology: The legacy of Wilfried Brutsaert adn Jean-Yves Parlange

    NARCIS (Netherlands)

    Troch, P.A.; Berne, A.; Bogaart, P.W.; Harman, C.; Hilberts, A.G.J.; Lyon, S.W.; Paniconi, C.; Pauwels, V.R.N.; Rupp, D.E.; Selker, J.S.; Teuling, A.J.; Uijlenhoet, R.; Verhoest, N.E.C.

    2013-01-01

    Based on a literature overview, this paper summarizes the impact and legacy of the contributions of Wilfried Brutsaert and Jean-Yves Parlange (Cornell University) with respect to the current state-of-the-art understanding in hydraulic groundwater theory. Forming the basis of many applications in cat

  7. Evaluation of a Successful High Risk Nursing Student Assistance Program: One ADN Program's Journey

    Science.gov (United States)

    O'Sullivan, Ciaran Anthony Mary

    2013-01-01

    A college education is, for many in America, part and parcel of the American Dream, and is certainly achievable. For countless reasons, students may enroll at community colleges underprepared, unprepared, anxious, and destined for a high risk of failure. Although community colleges are higher education institutions open and accessible to all who…

  8. Aspectos legales y éticos vinculados con el establecimiento y uso de los reservorios y bancos de ADN humano

    Directory of Open Access Journals (Sweden)

    Bianchi, Néstor Oscar

    2007-01-01

    Full Text Available La utilidad de los depósitos de material genético humano para la investigación médica, antropológica y básica es indiscutible. Los repositorios y bancos permiten compartir las muestras entre distintos laboratorios de investigación, efectuar nuevos ensayos moleculares en muestras representativas de distintos grupos étnicos o de diversas enfermedades genéticas o hereditarias, acelerar y abaratar la obtención de resultados evitando la implementación de colectas de material biológico cada vez que se inicia un nuevo proyecto científico. Sin embargo, la organización de un repositorio o banco de material genético es compleja, involucra a varias partes (donantes, representantes legales del repositorio o banco, usuarios del material depositado y a distintas entidades y organismos públicos y privados con y sin fines de lucro, tales como organismos que proveen los fondos para financiar los bancos y repositorios, entidades sede de los bancos y repositorios, comités de ética encargados de autorizar y monitorear los depósitos de material genético, empresas farmacéuticas que realizan investigaciones con fines de lucro, organizaciones no gubernamentales sin fines de lucro que colectan fondos para el establecimiento de reservorios de material genético destinados a mejorar la asistencia médica de determinadas afecciones. Esta multiplicidad de actores con intereses diversos genera también diversas interpretaciones de las cuestiones éticas y legales relacionadas con los depósitos de material genético, las cuales se hacen evidentes al analizar las recomendaciones sobre el tema que hacen distintas organizaciones internacionales tales como UNESCO, HUGO, OMS, Comités de Ética de la Comunidad Europea y de Australia, “American Society of Human Genetics”, “American College of Medical Genetics”.

  9. Oligomeric adiponectin forms and their complexes in the blood of healthy donors and patients with type 2 diabetes mellitus.

    Science.gov (United States)

    Kogan, Alexander E; Filatov, Vladimir L; Kolosova, Olga V; Katrukha, Ivan A; Mironova, Ekaterina V; Zhuravleva, Natalya S; Nagibin, Oleg A; Kara, Andrei N; Bereznikova, Anastasiya V; Katrukha, Alexey G

    2013-01-01

    Adiponectin (Adn) is a protein that circulates in the blood in several oligomeric forms, namely low-, medium-, and high-molecular-weight forms. Adn may serve as a risk factor for type 2 diabetes mellitus (T2DM). The aims of this work were (1) to produce monoclonal antibodies (MAbs) specific to different Adn oligomeric forms, (2) to design immunoassays suitable for measuring the Adn forms present in human blood, and (3) to investigate the changes in Adn forms that occur in patients with T2DM. Gel filtration, fluoroimmunoassays, and Western blotting were utilized as major techniques in this study. MAbs recognizing various oligomeric forms of Adn were obtained. Complexes between Adn and complement component C1q and between the low molecular weight form of Adn and albumin were described in human blood. A decrease in the total Adn and Adn-albumin complex levels in the blood of patients with T2DM and no difference in the levels of the Adn-C1q complex in comparison with healthy volunteers were demonstrated. An Adn94-Adn63 fluoroimmunoassay was selected as the technique that most accurately measured the mass ratio of Adn oligomers in blood samples, and an Adn214-Adn27 assay that measured the low-molecular-weight form of Adn only.

  10. Institutional Goal Priorities in Texas: A Look at an Associate Degree Nursing Program.

    Science.gov (United States)

    De Leon, John E.

    A study examined the perceptions of four key constituent groups from the Southeast College Associate Degree Nursing (ADN) program regarding institutional goal priorities. (Southeast College manages the ADN program for the Houston Community College System.) The study involved 23 ADN faculty, 13 college administrators, 128 ADN students, and 5 ADN…

  11. [Potentialities of computed tomography and ultrasound in diagnosis of hormonally active adrenal diseases: results of comparison CT and US with operative adn histological data].

    Science.gov (United States)

    Denisova, L B; Vorontsova, S V; Emel'ianova, L N

    2000-01-01

    The data given in the paper suggest that X-ray computed tomography (CT) is highly effective in detecting all types of hormonally active adrenal abnormalities. CT used in hormonally active adrenal diseases yielded data on major quantitative and qualitative (primarily densitometric) criteria that could be used in assessing the images of the adrenal area in these patients. Ultrasound study (USS) made at the first stage of topical diagnostic searches was of informative value in detecting adrenal tumor lesions, the technique being highly sensitive in the diagnosis of adrenal pheochromocytomas and adenocarcinomas, but less informative in the detection of hormonally active adrenocortical adenomas (aldesterone-producing ones in particular) than CT. The diagnosis of various adrenocortical hyperplasies and the differentiation of hyperplastic and tumor forms of hypercorticoidism are a prerogative of CT that substantially supplements USS findings in such cases.

  12. Ouverture mécanique de la molécule d'ADN par micro-manipulation et mesure de force

    Science.gov (United States)

    Bockelmann, Ulrich; Essevaz-Roulet, B.; Thomen, Philippe; Heslot, François

    In this paper we summarize part of our work on the mechanical unzipping of DNA. We have prepared molecular constructions which allow us to attach the two complementary strands of one end of a single DNA molecule of the bacteriophage λ separately to a glass microscope slide and a microscopic bead. In a first series of experiments, a soft microneedle acting as a force sensor is attached to the bead and its deflection is measured with an optical microscope. In a second series, we use an optical trapping interferometer to capture the bead and to measure its displacement to nm resolution. The sample is slowly displaced with respect to the force measurement device, leading to a progressive opening of the double helix. The force measured during this mechanical opening shows a characteristic variation which is related to the base pair sequence of the DNA molecule. To cite this article: U. Bockelmann et al., C. R. Physique 3 (2002) 585-594.

  13. Assortive mating for personaltiy traits, educational level, religious affiliation, height, weight, adn body mass index in parents of Korean twin sample.

    Science.gov (United States)

    Hur, Yoon-Mi

    2003-12-01

    The degree of assortative mating for psychological and physical traits in Asian societies in relatively unknown. The present study examined assortative mating for educational level, personality traits, religious affiliation, height, weight, and body mass index in a korean sample. Age-adjusted spouse correlations were high for educational level (r = .63) and religious affiliation (r = .67), modest for most personality traits (rs = -.01 to .26), and trivial for height (r = .04), weight (r = .05)m and body mass index (r = .11). These results were remarkably similar to those found from the western samples. Implications of the present findings in behavior genetic studies and human mating patterns were briefly discussed.

  14. Additional case of Marden-Walker syndrome: support for the autosomal-recessive inheritance adn refinement of phenotype in a surviving patient.

    Science.gov (United States)

    Orrico, A; Galli, L; Zappella, M; Orsi, A; Hayek, G

    2001-02-01

    In this report, we present a 14-year-old girl, born to consanguineous parents, who presented with severe mental retardation, hypotonia, short stature, and congenital joint contractures. The craniofacial features were scaphocephaly, thin/long and immobile face, marked hypoplasia of the midface, temporal narrowness, blepharophimosis, palpebral ptosis, and strabismus. The combination of such a distinctive craniofacial appearance and psychomotor retardation allows us to recognize a new case of the Marden-Walker syndrome. Our patient represents one of the rare cases in which consanguineous mating supports the autosomal-recessive pattern of inheritance of this condition. Furthermore, through refining the phenotype of a surviving patient, this report may contribute to a better recognition of this disorder in older affected children.

  15. Early Identification of Transformation in the Proficiency Level of Critical Thinking Skills (CTS) for the First Semester Associate Degree Nursing (ADN) Student

    Science.gov (United States)

    Swing, Velmarie K.

    2014-01-01

    Critical thinking (CT) in the new nursing graduate continues to be a topic of concern in the academic and acute care settings. While research studies have analyzed critical thinking skills (CTS) at the beginning and end of nursing programs, few have focused on early program evaluation of CT. In this non-experimental, explanatory, quantitative…

  16. Different uptake of 99mTc-ECD adn 99mTc-HMPAO in the same brains: analysis by statistical parametric mapping.

    Science.gov (United States)

    Hyun, Y; Lee, J S; Rha, J H; Lee, I K; Ha, C K; Lee, D S

    2001-02-01

    The purpose of this study was to investigate the differences between technetium-99m ethyl cysteinate dimer (99mTc-ECD) and technetium-99m hexamethylpropylene amine oxime (99mTc-HMPAO) uptake in the same brains by means of statistical parametric mapping (SPM) analysis. We examined 20 patients (9 male, 11 female, mean age 62+/-12 years) using 99mTc-ECD and 99mTc-HMPAO single-photon emission tomography (SPET) and magnetic resonance imaging (MRI) of the brain less than 7 days after onset of stroke. MRI showed no cortical infarctions. Infarctions in the pons (6 patients) and medulla (1), ischaemic periventricular white matter lesions (13) and lacunar infarction (7) were found on MRI. Split-dose and sequential SPET techniques were used for 99mTc-ECD and 99mTc-HMPAO brain SPET, without repositioning of the patient. All of the SPET images were spatially transformed to standard space, smoothed and globally normalized. The differences between the 99mTc-ECD and 99mTc-HMPAO SPET images were statistically analysed using statistical parametric mapping (SPM) 96 software. The difference between two groups was considered significant at a threshold of uncorrected P values less than 0.01. Visual analysis showed no hypoperfused areas on either 99mTc-ECD or 99mTc-HMPAO SPET images. SPM analysis revealed significantly different uptake of 99mTc-ECD and 99mTc-HMPAO in the same brains. On the 99mTc-ECD SPET images, relatively higher uptake was observed in the frontal, parietal and occipital lobes, in the left superior temporal lobe and in the superior region of the cerebellum. On the 99mTc-HMPAO SPET images, relatively higher uptake was observed in the medial temporal lobes, thalami, periventricular white matter and brain stem. These differences in uptake of the two tracers in the same brains on SPM analysis suggest that interpretation of cerebral perfusion is possible using SPET with 99mTc-ECD and 99mTc-HMPAO.

  17. Étude des dommages induits dans l'ADN par irradiation laser X-UV à 21.2 nm

    Science.gov (United States)

    Cassou, K.; Ros, D.; Kazamias, S.; Klisnick, A.; Jamelot, G.; Guilbaud, O.; Rus, B.; Kozlová, M.; Polan, J.; Präg, A. R.; Stupka, M.; Eot-Houllier, G.; Sage, E.; Begusová, M.; Stísová, V.; Du Penhoat, M.-A. H.; Touati, A.; Chétioui, A.

    2005-06-01

    We report the preliminary result of the application of the collisional Ne-like soft x-ray laser as radiation source to induce DNA damage. The goal of this experiment was a test bed study of the damage yields induced by soft x-ray radiation in dried plasmid DNA sample. The saturated Ne-like soft x-ray laser available at the PALS facility, delivering several millijoules in a single 100 ps pulse at 21.2 nm was used to irradiate two different plasmids: pSP189 and pBS. The study is centered on the dose effect leading to single and double strand break in DNA.

  18. [Unification of methods for determining the trace quantities of lead, zinc, sodium and potassium ions in the assessment of drinking water adn transfusion fluid quality].

    Science.gov (United States)

    Popkov, V A; Golovina, N V; Evgrafov, A A

    2001-01-01

    The results of long-term studies made by the Department of General Chemistry, I. M. Sechenov Moscow Medical Academy, that deals with unification of methods for determining some ions of metals (lead, zinc, sodium, and potassium) in the assessment of the quality of drinking water and transfusion fluids are summarized. A procedure was developed to determine the trace impurities of zinc, lead, and silver by atomic absorption spectrometry (AAS) by using sorption concentration. C-80-2-aminothiazole, a new sorbent synthesized at the Research Institute of Polymers, was used to detect these ions in the drinking water. With regards to the chosen optimal conditions, drinking water samples were analyzed via their direct spraying in the air-acetylene flame. The prior sorption concentration determined drinking water zinc and lead ions in the concentrations equal to or less than their permissible dose concentrations. The studies indicated that the used methods to determine the trace quantities of metals in the drinking water and aqueous solutions show a high sensitivity, rapidity, simplicity of sample preparation.

  19. Corrosion and protection of heterogeneous cast Al-Si (356) and Al-Si-Cu-Fe (380) alloys by chromate adn cerium inhibitors

    Science.gov (United States)

    Jain, Syadwad

    In this study, the localized corrosion and conversion coating on cast alloys 356 (Al-7.0Si-0.3Mg) and 380 (Al-8.5Si-3.5Cu-1.6Fe) were characterized. The intermetallic phases presence in the permanent mold cast alloy 356 are primary-Si, Al5FeSi, Al8Si6Mg3Fe and Mg2Si. The die cast alloy 380 is rich in Cu and Fe elements. These alloying elements result in formation of the intermetallic phases Al 5FeSi, Al2Cu and Al(FeCuCr) along with primary-Si. The Cu- and Fe-rich IMPS are cathodic with respect to the matrix phase and strongly govern the corrosion behavior of the two cast alloys in an aggressive environment due to formation of local electrochemical cell in their vicinity. Results have shown that corrosion behavior of permanent mould cast alloy 356 is significantly better than the die cast aluminum alloy 380, primarily due to high content of Cu- and Fe-rich phases such as Al2Cu and Al 5FeSi in the latter. The IMPS also alter the protection mechanism of the cast alloys in the presence of inhibitors in an environment. The presence of chromate in the solution results in reduced cathodic activity on all the phases. Chromate provides some anodic inhibition by increasing pitting potentials and altering corrosion potentials for the phases. Results have shown that performance of CCC was much better on 356 than on 380, primarily due to inhomogeneous and incomplete coating deposition on Cu- and Fe- phases present in alloy 380. XPS and Raman were used to characterize coating deposition on intermetallics. Results show evidence of cyanide complex formation on the intermetallic phases. The presence of this complex is speculated to locally suppress CCC formation. Formation and breakdown of cerium conversion coatings on 356 and 380 was also analyzed. Results showed that deposition of cerium hydroxide started with heavy precipitation on intermetallic particles with the coatings growing outwards onto the matrix. Electrochemical analysis of synthesized intermetallics compounds in the presence of soluble cerium cations showed that of anodic and cathodic activity was not as strongly inhibited as was observed for chromate ions. Overall cerium conversion coating showed good performance on Al-Si (356) ally, but poor performance on Fe- and Cu-rich alloy (380).

  20. Estudio de la diversidad genética intrarracial de la cabra murciano-granadina con microsatélites de ADN

    OpenAIRE

    Rocha, L.L. da; Martínez Martínez, Amparo; Delgado-Bermejo, J.V.; Quiroz, J.

    2007-01-01

    Granadina goats, Murciana goats and their crosses are included in the same genealogical book that is managed by two different breeders associations: the Asociación Nacional de Criadores de Caprinos de Raza Murciano-Granadina and the Asociación de Criadores de Cabras Murcianas ACRIMUR. Both populations form the Murciano-Granadina breed, reported in this way in the Catálogo Oficial de Razas de Ganado de España. This common genetic management has had excellent benefits like the international rec...

  1. Estudio de la diversidad genética intrarracial de la cabra murciano-granadina con microsatélites de ADN

    OpenAIRE

    A.M. Martínez; Rocha, L.; Quiroz, J.; DELGADO, J. V.

    2007-01-01

    Actualmente tanto las cabras Granadinas como las cabras Murcianas, así como sus cruces se inscriben en un único libro genealógico gestionado por dos asociaciones de criadores: la Asociación Nacional de Criadores de Caprinos de Raza Murciano-Granadina y la Asociación de Criadores de Cabras Murcianas ACRIMUR. Oficialmente ambas poblaciones se integran en una única raza llamada Murciano-Granadina, incluida como tal en el catálogo Oficial de Razas de Ganado de España. Esta gestión genética común ...

  2. Amplificación de la señal Raman de ADN genómico utilizando microvaras de ZnO

    OpenAIRE

    Guzmán Embús, Diego Alonso

    2015-01-01

    En este trabajo es presentado los resultados en los procesos de obtención de varillas de ZnO usando las rutas químicas Sol Gel, Hidrotérmico y SILAR-Hidrotérmico, obteniendo polvos y películas de ZnO. Se realizaron análisis por difracción de rayos X, espectroscopia Raman, espectroscopia UV-Visible y microscopia electrónica de Barrido de las muestras obtenidas. Fue realizado un estudio de la influencia que tiene la incorporación de cadmio para el crecimiento de las varillas de ZnO, encontrando...

  3. Raman spectra of ethane adn ethylene adsorbed of surface of catalyst Sm 2 O 3/MgO at high temperatures

    Science.gov (United States)

    Bobrov, A. V.; Plate, S. E.; Kadushin, A. A.; Kimel'feld, Ya. M.; Seleznev, V. A.; Tulenin, Yu. P.

    1992-03-01

    Raman spectroscopy has been applied for the investigation in situ of catalytic transformations of ethane ethylene and deuterated ethylene on the surface of catalyst Sm 2O 3/MgO at high temperatures (up to 980 K). Ethane and ethylene are the products of the oxidation dehydration of methane. For the understanding of mechanism of this process it is interesting to study of the spectra of ethane and ethylene in the conditions of the real process at high temperature. In the previous paper [1] we have shown that during the flowring of mixture methane with oxygen through the catalyst Sm 2O 3/MgO methane dissociates on CH 3 and CH 2 groups. It has been confirmed by experiment with deuterated methane. The purpose of this work is to study the interaction of ethane and ethylene with Sm 2O 3/MgO in similar conditions by Raman spectroscopy.

  4. Diversidad genética entre especies del género lemna (lemnaceae utilizando fragmentos polimórficos de ADN amplificados al azar (rapds

    Directory of Open Access Journals (Sweden)

    Frank Solano Campos

    2016-03-01

    Full Text Available Lemna aequinoctialis and Lemna valdiviana were tested genetic similarity by random amplified polymorphic DNA (RAPD fingerprinting. Of 60 primers (10-mer tested, 26 generated polymorphic products. 143 bands were found from 200bp up to 1500bp, 109 were polymorphic (76,2%, with an average of 4,19. Of these, only 18 were specific of L. aequinoctialis, while 91 bands were specific for L. valdiviana. Data were used to generate Jaccard’s similarity coefficients and to construct a dendrogram using UPGMA method in the BiodiversityPro statistical package. It is concluded from this study that there were clear differences (high genetic diversity between L. aequinoctialis and L. valdiviana and that RAPD sesults are comparable with those obtained from studies on morphology, It is a practical method to assess the relationships between these species.

  5. Antioxidant effect of naturally occurring xanthines on the oxidative damage of DNA bases; Effet antioxydant de xanthines naturelles sur le dommage oxydant des bases de l`ADN

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, A.J.S.C.; Telo, J.P.; Pereira, H.F.; Patrocinio, P.F. [Instituto Superior Tecnico, Lisbon (Portugal); Dias, R.M.B. [Instituto Tecnologico e Nuclear, Sacavem codex (Portugal). Dept. de Quimica

    1999-01-01

    The repair of the oxidised radicals of adenine and guanosine by several naturally occurring xanthines was studied. Each pair of DNA purine/xanthine was made to react with the sulphate radical and the decrease of the concentration of both compounds was measured by HPLC as a function of irradiation time. The results show that xanthine efficiently prevents the oxidation of the two DNA purines. Theophylline and para-xanthine repair the oxidizes radical of adenine but not the one from guanosine. Theobromine and caffeine to do not show any protecting effect. An order of the oxidation potentials of all the purines studied is proposed. (authors) 10 refs.

  6. Teachers' self-efficacy, motivation adn teaching strategies - Auto-eficacia Docente, Motivación del Profesor y Estrategias de Enseñanza

    Directory of Open Access Journals (Sweden)

    Pedro Rosario

    2009-12-01

    Full Text Available According to previous research, teachers’ efficacy relating to teaching practices is highly dependent on the extent to which they are confident about their own capabilities to manage the new demands on their professional role. The present work aimed at: a gathering information about the way teachers manage different aspects of self-efficacy (i.e., enhancing their instructional process in order to optimize their lessons and engage the students in the learning process, in order to identify homogeneous self-efficacy profiles, b investigating the way these profiles relate to different levels and types of motivation, teaching strategies and teachers’ self-esteem. Ninety-five teachers from five Spanish public Universities participated in this study. Three different profiles of teachers’ efficacy have been identified: 1 high self-efficacy in the three dimensions; 2 medium self-efficacy in the enhancement of the teaching process and of lessons management, and medium-high efficacy in the engagement of students, and 3 low self-efficacy in the three dimensions. ANOVA results supported previous findings since teachers’ self-efficacy beliefs played a crucial role on teachers’ motivation and professional engagement.

  7. Properties of ADN and Its Application in Monopropellants%ADN的性质及其在单元推进剂中的应用

    Institute of Scientific and Technical Information of China (English)

    王学敏; 刘海洲; 许华新; 陈兴强; 王连心

    2014-01-01

    介绍了目前最有希望取代肼的一类单元推进剂——离子盐溶液推进剂.结合ADN高能、高氧含量和强吸湿性的特点,指出了ADN在新型离子盐溶液推进剂中的应用前景.综述了瑞典在ADN单元推进剂配方研究、发动机试验和高纯ADN生产等方面的进展,对国内ADN无毒推进技术研究提出了建议.

  8. 燃速催化剂对ADN的热分解作用%The Effects of Combustion Catalyst on the Thermal Decomposition of ADN

    Institute of Scientific and Technical Information of China (English)

    万代红; 府勤; 黄洪勇; 卢国强

    2006-01-01

    用线性升温条件下的差热分析、热失重以及恒温热失重研究了近10种燃速催化剂对ADN热分解性能的影响.用4个不同升温速率下的DTA实验结果,计算了ADN及其与催化剂混合物的热分解动力学参数.结果表明,Cu、Fe类催化剂(除Fe2O3外)对ADN的热分解作用明显,大大降低了ADN的热分解峰温,加快了ADN的失重速率,其中Cu类催化剂对ADN的催化效果最为显著,其催化机理为凝聚相催化;Fe类催化剂属气相催化.而铵盐、Ca盐催化剂则由于分解时放出NH3,抑制了ADN的质子转移或生成了稳定性高的Ca盐,提高了ADN的分解峰温和活化能,对ADN的热分解起到抑制作用.

  9. Optimización de la extracción de ADN de Passiflora ligularis para el análisis por medio de marcadores moleculares

    Directory of Open Access Journals (Sweden)

    Gina Solano-Flórez

    2009-04-01

    Full Text Available Extraction of high quality DNA of Passiflora ligularis for its analysis with molecular markers. Objective. To standardize a precise andefficient DNA isolation protocol of Passiflora ligularis. Materials and methods. Two methods of DNA extraction and two different tissuesof Passiflora ligularis were evaluated in terms of purity, quality and quantity of DNA yield, as well as DNA’s suitability for moleculartechniques based on PCR such as RAPDs. Quantification of DNA was done using absorbance spectrophotometry at a wavelength of260nm (A260 with a Beckman Du ® 530 spectrophotometer. An estimate of the DNA’s purity was obtained using the absorbance ratio(A260 / A280 nm. The variables analyzed in this study were the extraction method (A and the tissue type (B, in order to define their influenceon the purity and quantity of the DNA extracted. For the study of these variables a random design with a 2 x 2 factorial arrangement wasused. Results. The average quantities of DNA obtained with the modified method of Mc Couch et al. (1988 and the modified method ofDoyle & Doyle (1991 method were 778.9 μg/ml and 660.1 μg/ml respectively for dry tissue. Averages with fresh tissue were 1543.3 μg/ml and 820.4 μg/ml respectively. Conclusion. Based upon our results we suggest the use of Mc Couch et al. (1988 method with fresh leaftissue to obtain a high quality DNA suitable to be used with RAPDs molecular markers.

  10. ESTUDIO DE LA INTERACCION DE CATIONES METALICOS CON BASES DEL ADN DESDE LA PERSPECTIVA DE LA TEORIA DE FUNCIONALES DE LA DENSIDAD CONCEPTUAL

    OpenAIRE

    RINCON BEDOYA, ELIZABETH ADRIANA

    2007-01-01

    Utilizando los métodos computacionales y herramientas coiiceptuales enmarcados dentro de la teoría de funcionales de la densidad conceptual, se ha estudiado la iiiteracción de cationes metálicos divalentes, representativos y de transición, Mg(lI), Cu(II), 146p.

  11. Repair mechanisms inducible to the DNA in I.M.M.S. biological systems; Mecanismos de reparacion inducible del ADN en sistemas biologicos I.M.M.S

    Energy Technology Data Exchange (ETDEWEB)

    Guzman, J.; Arceo, C.; Cortinas, C.; Rosa, M.E. De la; Olvera, O.; Cruces, M.; Pimentel, E

    1990-03-15

    Given the characteristics of the MMS and the relative antecedents to the repair mechanisms in eucariontes are sought to determine the effect of the MMS on the genetic material and their repair in Drosophila melanogaster. (Author)

  12. Fragmentación del ADN espermático en muestras procedentes de clínicas de reproducción asistida

    OpenAIRE

    Molinero García, Natalia

    2014-01-01

    La esterilidad afecta aproximadamente a un 20% de las parejas que se encuentran en edad reproductiva. En el caso del varón, en los laboratorios de andrología se realizan análisis que de forma rutinaria estudian el volumen, concentración espermática, motilidad y morfología, según los criterios establecidos por la Organización Mundial de la Salud. Sin embargo, el estudio de éstas características no siempre es concluyente. Es necesario añadir el estudio de otros parámetros, como en nuestro cas...

  13. Extracción y purificación del adn de moniliophthora roreri hongo que ataca el cacao, en norte de santander

    Directory of Open Access Journals (Sweden)

    Liliana Yanet Suárez Contreras

    2005-07-01

    Full Text Available This research has as objetive to define the methodology of extraction and purification for Moniliophthora roreri and to apply it to 56 isolations of Moniliophthora roreri obtained from Cúcuta, Agua Clara, Sardinata, El Tarra, Tibú, Bucaracica, Teorama and Zulia in Norte de Santander (Colombia. The extraction of DNA was carried out by the protocol proposed by Miranda and Sandoval in 2000, with some proposed modifications by Rocha. To its purification was utilized Chloroform Phenol. Once, it was standarized by the protocol of extraction. It tested with other mushrooms:Metarhizium sp, Botritys cincrea, Fusarium culmorum, Phytophthora cinnamomi. This work intends to continue with the research in the area of Molecular Biology of Moniliophthora roreri and other phytopathogens of economic importance for the region, it promotes the research in micology.

  14. Desmetilación activa del ADN: un mecanismo epigenético para la reactivación de genes silenciados

    OpenAIRE

    Ariza, R R; Roldán-Arjona, T.; García-Ortiz, M.V.; Morales-Ruiz, T.; Ortega-Galisteo, A.P.; Martínez-Macías, M.I.; Ponferrada-Marín, M.I.; Schiliro, E.

    2009-01-01

    Los mecanismos de control epigenético son esenciales para una regulación estable de los patrones de expresión génica y desempeñan un papel central en los ciclos de vida de animales y plantas. La metilación de la citosina en el carbono 5 del anillo de pirimidina (5-meC) es una marca epigenética estable, pero reversible, que promueve el silenciamiento génico transcripcional. Comprender cómo se regula el estado de metilación del genoma a nivel global o local requiere una definición d...

  15. La utilización del concepto de ADN en nuestra sociedad: tecnociencia, frases hechas y errores científicos

    Directory of Open Access Journals (Sweden)

    Francesc Mestres

    2012-01-01

    Full Text Available Technology based on DNA and its applications has revolutionized our society. Information regarding these advances has rapidly spread by the media. Furthermore, people have incorporated the DNA technology concepts in their worldview. Several set phrases based on DNA have had a depth media success, but someone contain severe scientific mistakes. This paper is focused in presenting this errors and proposing different ways to overcome them.

  16. CARACTERIZACIÓN AGROMORFOLOGICA Y CONTENIDO EN ADN NUCLEAR EN ACCESIONES DE CUATRO ESPECIES DE MEDICAGOS ANUALES DEL NORTE DE ESPAÑA

    OpenAIRE

    Oliveira Prendes, J. A.; AFIF KHOURI, E.

    2012-01-01

    Dieciséis accesiones anuales y autógamas de cuatro especies del género Medicago procedentes del norte de España se caracterizaron durante dos años (2008 y 2009) en un diseño por bloques aleatorizados completos con tres repeticiones de 10 plantas por accesión (en total 30 plantas por accesión). El cv Santiago de Medicago polymorpha y la accesión 3710 de Medicago arábica se incluyeron como testigos en el estudio. Todas las accesiones y los testigos se evaluaron mediante nueve caracteres agromor...

  17. Filogenética y genética de poblaciones de Potos Flavus (Carnivora: Procyonidae) con secuencias de ADN mitocondrial

    OpenAIRE

    2014-01-01

    Este es el primer estudio desarrollado sobre sistemática molecular, genética de poblaciones y filogeografía dentro de Potos flavus (Procyonidae) con un número representativo de muestras (n=100) y que abarca una parte importante de la distribución de este carnívoro neotropical.

  18. Repair of model compounds of photoinduced lesions in DNA. Electrochemical approaches; Reparation de modeles de lesions photoinduites de l'ADN. Approches electrochimiques

    Energy Technology Data Exchange (ETDEWEB)

    Boussicault, F

    2006-09-15

    The goal of this work is to better understand the repair mechanism of photoinduced lesions in DNA (cyclobutane dimers and pyrimidine (6-4) pyrimidone adducts) by photolyase redox enzymes, using tools and concepts of molecular electrochemistry. Thanks to the study of model compounds of cyclobutane lesions by cyclic voltametry, we have been able to mimic the key step of the enzymatic repair (dissociative electron transfer) and to monitor the repair of model compounds by Escherichia coli DNA photolyase. From these results, we have discussed the repair mechanism, especially the stepwise or concerted character of the process. Repair mechanism of (6-4) adducts is not known now, but a possible pathway implies an electron transfer coupled to the cleavage of two bonds in the closed form of the lesions (oxetanes). Voltammetric study of reduction and oxidation of model oxetanes and their repair by E. coli DNA photolyase gave some experimental evidence confirming the proposed mechanism and allowing a better understanding of it. (author)

  19. Amélioration de la méthode d'extraction d'ADN au CTAB appliquée aux feuilles de cotonnier

    Directory of Open Access Journals (Sweden)

    Mergeai G.

    2006-01-01

    Full Text Available Improvement of the genomic DNA extraction method with CTAB for cotton leaves. Molecular genetic analysis in cotton (Gossypium hirsutum L. is often limited by the availability of fresh tissue and the time necessary to extract DNA from it. To overcome these problems, the original CTAB method was improved. The major modifi cations concern DNA precipitation at -20°C, incubation of the resuspended DNA at 60°C and centrifugation at 4°C for all extraction steps. The improved method was relatively fast, cheap and yielded high quality DNA (80-200 μg . g-1. The optimized protocol gives satisfactory results on dried and frozen leaves at -80°C. The DNA was suitable for restriction-enzyme digestion with EcoRI and as a template for polymerase chain reaction (PCR using more than two hundred microsatellite cotton primers on different cotton species, the G. hirsutum × G. raimondii × G. sturtianum trispecifi c hybrid and its progenies.

  20. Analysis adn Design of City Intelligent Trangsport System%城市智能运输系统的分析与设计

    Institute of Scientific and Technical Information of China (English)

    寇有观

    2001-01-01

    The paper analyses the aims,function and data for cityintelligent transport system,and discusses strategic data planning and global system design,and constructs digital city intelligent transport system by introducing GIS and spatial database.%文章分析城市智能运输系统的目标、功能和数据,讨论城市智能运输系统的数据总体规划和系统总体设计。引进地理信息系统和空间数据库,构建数字城市智能运输系统。

  1. Affine Equivalence and Constructions of Cryptographically Strong Boolean Functions

    Science.gov (United States)

    2013-09-01

    3 (mod n) aiaj, . . . , n⊕ i, j=1 i+j≡n (mod n) aiaj, n⊕ i=1 i+j≡1 (mod n) aiaj  =  C(a1, adn /2e+1, a2, adn /2e+2, . . . , adn /2e) if...a3, . . . , ak, a2k+1, ak+1) = C(a1, adn /2e+1, a2, adn /2e+2, . . . , adn /2e). 46 If n = 2k for k = 0, 1, 2, ..., n⊕ i=1 i+j≡2 (mod n) aiaj = a1a1...3.4.5, C(a1, . . . , an) 2 = C(a1, adn /2e+1, a2, adn /2e+2, . . . , adn /2e) = C((a1, . . . , an)Pτ ) = C(aτ(1), . . . , aτ(n)). 64 Therefore, (a1

  2. The ratio of high-molecular weight adiponectin and total adiponectin differs in preterm and term infants.

    Science.gov (United States)

    Yoshida, Tomohide; Nagasaki, Hiraku; Asato, Yoshihide; Ohta, Takao

    2009-05-01

    Adiponectin consists of three subspecies (high-, middle- and low-molecular weight adiponectin). Among these, high-molecular weight adiponectin (H-adn) is suggested to be an active form of this protein. To assess the relationship between H-adn and postnatal growth in preterm infants (PIs), serum H-adn and total adiponectin (T-adn) were measured in 46 PIs at birth and at corrected term, and 26 term infants (TI) at birth. T-adn and H-adn concentrations, and the ratio of H-adn to T-adn (H/T-adn) were significantly greater in TI and PI at corrected term than in PI at birth (p adn and H-adn concentrations in PI at corrected term were similar to those in TI, but H/T-adn in PI at corrected term was less than that in TI (p adn and serum concentrations of T- and H-adn in PI at corrected term were different from those in TI. These data suggest that quality of early postnatal growth in PIs is different from that in normally developed TI. Postnatal growth accompanying adipose tissue similar to TI may be important for PI to prevent future development of cardiovascular disease.

  3. Analysis of damaged DNA / proteins interactions: Methodological optimizations and applications to DNA lesions induced by platinum anticancer drugs; Analyse des interactions ADN lese / proteines: Optimisations methodologiques et applications aux dommages de l'ADN engendres par les derives du platine

    Energy Technology Data Exchange (ETDEWEB)

    Bounaix Morand du Puch, Ch

    2010-10-15

    DNA lesions contribute to the alteration of DNA structure, thereby inhibiting essential cellular processes. Such alterations may be beneficial for chemotherapies, for example in the case of platinum anticancer agents. They generate bulky adducts that, if not repaired, ultimately cause apoptosis. A better understanding of the biological response to such molecules can be obtained through the study of proteins that directly interact with the damages. These proteins constitute the DNA lesions interactome. This thesis presents the development of tools aiming at increasing the list of platinum adduct-associated proteins. Firstly, we designed a ligand fishing system made of damaged plasmids immobilized onto magnetic beads. Three platinum drugs were selected for our study: cisplatin, oxali-platin and satra-platin. Following exposure of the trap to nuclear extracts from HeLa cancer cells and identification of retained proteins by proteomics, we obtained already known candidates (HMGB1, hUBF, FACT complex) but also 29 new members of the platinated-DNA interactome. Among them, we noted the presence of PNUTS, TOX4 and WDR82, which associate to form the recently-discovered PTW/PP complex. Their capture was then confirmed with a second model, namely breast cancer cell line MDA MB 231, and the biological consequences of such an interaction now need to be elucidated. Secondly, we adapted a SPRi bio-chip to the study of platinum-damaged DNA/proteins interactions. Affinity of HMGB1 and newly characterized TOX4 for adducts generated by our three platinum drugs could be validated thanks to the bio-chip. Finally, we used our tools, as well as analytical chemistry and biochemistry methods, to evaluate the role of DDB2 (a factor involved in the recognition of UV-induced lesions) in the repair of cisplatin adducts. Our experiments using MDA MB 231 cells differentially expressing DDB2 showed that this protein is not responsible for the repair of platinum damages. Instead, it appears to act as a positive mediator of their cytotoxicity. In the near future, the above mentioned microsystems will be adapted to the study of the interactome of other DNA lesions. (author)

  4. Cerebral Aneurysms

    Science.gov (United States)

    ... person's age, general health, pre-existing neurological conditions, adn time between rupture and medical attention. Early diagnosis ... person's age, general health, pre-existing neurological conditions, adn time between rupture and medical attention. Early diagnosis ...

  5. 78 FR 20115 - Agency Information Collection Activities; Submission for Office of Management and Budget Review...

    Science.gov (United States)

    2013-04-03

    ... Health and Human Services (the Secretary) will assign an ADN for devices that meet the eligibility criteria to be permitted to be sold for profit. The ADN is defined as the number of devices ``reasonably... during any calendar year exceeds the ADN. Section 520(m)(6)(C) of the FD&C Act provides that an...

  6. 78 FR 42788 - American Indians Into Nursing; Notice of Competitive Grant Applications for American Indians Into...

    Science.gov (United States)

    2013-07-17

    ... Nurses (Associate Degree in Nursing (ADN), Bachelor of Science in Nursing (BSN)) and Advance Practice... expenses; (3) provide a program that encourages nurses (DNP, MSN, BSN, ADN) to ] provide or continue to... accommodate ADN to BSN, BSN to MSN, and MSN to DNP students. 7. Programs which have a faculty exchange...

  7. 75 FR 7270 - Agency Information Collection Activities; Submission for Office of Management and Budget Review...

    Science.gov (United States)

    2010-02-18

    ...) provides that the Secretary of Health and Human Services will assign an annual distribution number (ADN) for devices indicated for use in a pediatric population or in a pediatric subpopulation. The ADN shall... year exceeds the ADN. Section 520(m)(6)(C) provides that an HDE holder may petition to modify the...

  8. 77 FR 74667 - Agency Information Collection Activities; Proposed Collection; Comment Request; Information To...

    Science.gov (United States)

    2012-12-17

    ... annual distribution number (ADN). DATES: Submit either electronic or written comments on the collection... that the Secretary of Health and Human Services (the Secretary) will assign an ADN for devices that meet the eligibility criteria to be permitted to be sold for profit. The ADN is defined as the...

  9. Impaired endothelial function and insulin action in first-degree relatives of patients with type 2 diabetes mellitus

    DEFF Research Database (Denmark)

    Sonne, Mette P; Højbjerre, Lise; Alibegovic, Amra A

    2009-01-01

    . Forearm blood flow (FBF) was measured by venous occlusion plethysmography upon stimulation with systemic hyperinsulinemia (291 +/- 11 pmol/L, pooled data from both groups) and upon intraarterial infusion of adenosine (ADN) and acetylcholine (ACH) +/- hyperinsulinemia. Forearm blood flow response to ADN...... and ACH was less in FDR vs CON (P ADN in CON (P

  10. Review of Ammonium Dinitramide Toxicity Studies

    Science.gov (United States)

    2011-01-01

    reproductive toxicant in rats, causing implantation failure in early gestation; follow-on studies implied that ADN is embryotoxic . EPR studies indicated that...mutagenic. 15. SUBJECT TERMS Ammonium dinitramide, ADN, toxicity, reproductive, embryotoxic , genotoxicity 16. SECURITY CLASSIFICATION OF: U 17...reproductive studies, reproduction and fertility, pre-implantation and post-implantation studies, implied that ADN is embryotoxic . A mouse embryo

  11. Construction of adiponectin-encoding plasmid DNA and gene therapy of non-obese type 2 diabetes mellitus.

    Science.gov (United States)

    Nan, Mei Hua; Park, Jeong-Sook; Myung, Chang-Seon

    2010-01-01

    Adiponectin (ADN), an insulin-sensitizing adipokine, stimulates glucose uptake, inhibits gluconeogenesis, and plays an important role in improving insulin sensitivity. Since blood levels of ADN are low in type 2 diabetes mellitus (DM), this study was designed to investigate the therapeutic effectiveness of increasing the ADN level through injection of plasmid DNA encoding ADN in type 2 DM. A non-obese type 2 DM mouse model was established via combined administration of streptozotocin with nicotinamide and exhibited significantly higher plasma glucose concentration and insulin resistance compared with normal controls according to oral glucose tolerance and insulin challenge tests. Plasmid DNA encoding mouse ADN from differentiated NIH3T3 adipocytes was constructed in pVAX1 (pVAX/ADN). Transfection of pVAX/ADN into various cell lines including HeLa, HT22, HEK293, HepG2, and SK-Hep1 cells, increased ADN mRNA expression levels in a dose-dependent manner. The administration of pVAX/ADN into non-obese type 2 DM mice via tail vein significantly increased the blood level of ADN and decreased the plasma glucose concentration. Moreover, the parameters related to insulin resistance (HOMA-IR) and insulin sensitivity (QUICKI) were significantly improved. These results suggest that ADN gene therapy could be a clinically effective tool for the treatment of type 2 DM.

  12. Adiponectin inhibits insulin function in primary trophoblasts by PPARα-mediated ceramide synthesis.

    Science.gov (United States)

    Aye, Irving L M H; Gao, Xiaoli; Weintraub, Susan T; Jansson, Thomas; Powell, Theresa L

    2014-04-01

    Maternal adiponectin (ADN) levels are inversely correlated with birth weight, and ADN infusion in pregnant mice down-regulates placental nutrient transporters and decreases fetal growth. In contrast to the insulin-sensitizing effects in adipose tissue and muscle, ADN inhibits insulin signaling in the placenta. However, the molecular mechanisms involved are unknown. We hypothesized that ADN inhibits insulin signaling and insulin-stimulated amino acid transport in primary human trophoblasts by peroxisome proliferator-activated receptor-α (PPARα)-mediated ceramide synthesis. Primary human term trophoblast cells were treated with ADN and/or insulin. ADN increased the phosphorylation of p38 MAPK and PPARα. ADN inhibited insulin signaling and insulin-stimulated amino acid transport. This effect was dependent on PPARα, because activation of PPARα with an agonist (GW7647) inhibited insulin signaling and function, whereas PPARα-small interfering RNA reversed the effects of ADN on the insulin response. ADN increased ceramide synthase expression and stimulated ceramide production. C2-ceramide inhibited insulin signaling and function, whereas inhibition of ceramide synthase (with Fumonisin B1) reversed the effects of ADN on insulin signaling and amino acid transport. These findings are consistent with the model that maternal ADN limits fetal growth mediated by activation of placental PPARα and ceramide synthesis, which inhibits placental insulin signaling and amino acid transport, resulting in reduced fetal nutrient availability.

  13. The C-terminal N-glycosylation sites of the human alpha1,3/4-fucosyltransferase III, -V, and -VI (hFucTIII, -V, adn -VI) are necessary for the expression of full enzyme activity.

    Science.gov (United States)

    Christensen, L L; Jensen, U B; Bross, P; Orntoft, T F

    2000-09-01

    The alpha1,3/4-fucosyltransferases are involved in the synthesis of fucosylated cell surface glycoconjugates. Human alpha1,3/4-fucosyltransferase III, -V, and -VI (hFucTIII, -V, and -VI) contain two conserved C-terminal N-glycosylation sites (hFucTIII: Asn154 and Asn185; hFucTV: Asn167 and Asn198; and hFucTVI: Asn153 and Asn184). In the present study, we have analyzed the functional role of these potential N-glycosylation sites, laying the main emphasis on the sites in hFucTIII. Tunicamycin treatment completely abolished hFucTIII enzyme activity while castanospermine treatment diminished hFucTIII enzyme activity to approximately 40% of the activity of the native enzyme. To further analyze the role of the conserved N-glycosylation sites in hFucTIII, -V, and -VI, we made a series of mutant genomic DNAs in which the asparagine residues in the potential C-terminal N-glycosylation sites were replaced by glutamine. Subsequently, the hFucTIII, -V, and -VI wild type and the mutants were expressed in COS-7 cells. All the mutants exhibited lower enzyme activity than the wild type and elimination of individual sites had different effects on the activity. The mutations did not affect the protein level of the mutants in the cells, but reduced the molecular mass as predicted. Kinetic analysis of hFucTIII revealed that lack of glycosylation at Asn185 did not change the Km values for the oligosaccharide acceptor and the nucleotide sugar donor. The present study demonstrates that hFucTIII, -V, and -VI require N-glycosylation at the two conserved C-terminal N-glycosylation sites for expression of full enzyme activity.

  14. Modélisation théorique des expériences de molécules uniques sur l'ADN et l'ARN : de l'élasticité au dégraffage des bases

    Science.gov (United States)

    Cocco, Simona; Marko, John F.; Monasson, Rémi

    We review statistical-mechanical theories of single-molecule micromanipulation experiments on nucleic acids. Firstly, models for describing polymer elasticity are introduced. We then review how these models are used to interpret single-molecule force-extension experiments on single-stranded and double-stranded DNA. Depending on the force and the molecules used, both smooth elastic behavior and abrupt structural transitions are observed. Thirdly, we show how combining the elasticity of two single nucleic acid strands with a description of the base-pairing interactions between them explains much of the phenomenology and kinetics of RNA and DNA 'unzipping' experiments. To cite this article: S. Cocco et al., C. R. Physique 3 (2002) 569-584.

  15. Detection, characterization and measure of a new radiation-induced damage in isolated and cellular DNA; Detection, caracterisation et mesure d'un nouveau dommage radio-induit de l'ADN isole et cellulaire

    Energy Technology Data Exchange (ETDEWEB)

    Regulus, P

    2006-10-15

    Deoxyribonucleic acid (DNA) contains the genetic information and chemical injury to this macromolecule may have severe biological consequences. We report here the detection of 4 new radiation-induced DNA lesions by using a high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) approach. For that purpose, the characteristic fragmentation of most 2'-deoxy-ribo nucleosides, the loss of 116 Da corresponding to the loss of the 2-deoxyribose moiety, was used in the so-called neutral loss mode of the HPLC-MS/MS. One of the newly detected lesions, named dCyd341 because it is a 2'-deoxycytidine modification exhibiting a molecular weight of 341 Da, was also detected in cellular DNA. Characterization of this modified nucleoside was performed using NMR and exact mass determination of the product obtained by chemical synthesis. A mechanism of formation was then proposed, in which the first event is the H-abstraction at the C4 position of a 2-deoxyribose moiety. Then, the sugar modification produced exhibits a reactive aldehyde that, through reaction with a vicinal cytosine base, gives rise to dCyd341. dCyd341 could be considered as a complex damage since its formation involves a DNA strand break and a cross-link between a damaged sugar residue and a vicinal cytosine base located most probably on the complementary DNA strand. In addition to its characterization, preliminary biological studies revealed that cells are able to remove the lesion from DNA. Repair studies have revealed the ability of cells to excise the lesion. Identification of the repair systems involved could represent an interesting challenge. (author)

  16. Optimalisation de l'extraction d'ADN génomique de la morelle jaune (Solanum elaeagnifolium Cav., une plante invasive des milieux cultivés en région méditerranéenne

    Directory of Open Access Journals (Sweden)

    Ripoll, J.

    2011-01-01

    Full Text Available Optimization of the genomic DNA extraction method of silverleaf nightshade (Solanum elaeagnifolium Cav., an invasive plant in the cultivated areas within the Mediterranean region. The geographical origin of an invasive plant in the cultivated area within the Mediterranean region, silverleaf nightshade, Solanum elaeagnifolium Cav. (Solanaceae should be identified through the analysis of genetic similarities between native and introduced populations using microsatellite markers. Beforehand, an effective and less costly method for extracting genomic DNA from dehydrated and polysaccharide-rich leaves was investigated. The original Doyle's protocol based on the use of a cationic detergent, CTAB, was successfully optimized in order to extract high quality DNA (A260/280 ratio of 1.8; A260/230 ratio of 1.7 to 2. The DNA yields obtained with this new protocol were higher than those obtained with a commercial kit, although quality of the DNA extracted was comparable. The improvement of the protocol was further proven by the amplification of several microsatellite loci first developed in eggplant, Solanum melongena L.

  17. THE EXPERIMENTAL OF COMBINATIVE CHEMOTHERAPY OF CDDP ADN 5-FU IN ORAL SQUAMOUS CARCINOMA CELL LINE%顺铂和氟脲嘧啶联合应用抗口腔鳞癌的实验研究

    Institute of Scientific and Technical Information of China (English)

    王安训; 温映萍; 李苏; 黄洪章

    2003-01-01

    目的研究顺铂(cisplatin, CDDP)和5-氟脲嘧啶(5-fluorouracil, 5-FU)联合化疗对口腔鳞癌(Tca8113 细胞株)的体内外抗瘤作用.方法应用CDDP和5-FU单独或联合治疗口腔鳞癌细胞(Tca8113 细胞系)及其裸鼠移植瘤,采用MTT法检测其体外杀伤作用;观察移植瘤的生长情况及组织病理学改变;高效液相色谱法(HPLC)检测血液及移植瘤内5-FU的浓度.结果 CDDP和5-FU均对口腔鳞癌细胞具有强的体外杀伤作用,其IC50分别为:0.59μmol/L和2.33μmol/L.与对照组比较,移植瘤经CDDP和5-FU单独或联合治疗后生长均明显受到抑制(P<0.001),其抑瘤率分别为:57.0%(5-FU)、84.4%(CDDP)和97.2%(5-FU+CDDP),CDDP和5-FU存在协同的抑瘤作用(P<0.001);组织病理学显示各治疗组移植瘤内出现囊性变和灶性坏死;肿瘤组织和血液中可检测到高浓度的5-FU.结论 CDDP和5-FU联合化疗可能是口腔鳞癌有效的化疗方案.

  18. Aplicación de técnicas moleculares basadas en ADN y ARN al estudio de la diversidad microbiana en la cueva de Altamira (Cantabria, España)

    OpenAIRE

    Portillo Guisado, María del Carmen

    2007-01-01

    En los ecosistemas naturales conviven una gran diversidad de microorganismos (Pace, 1997; Delong, 2001). Hoy en día, se ha comprobado que los microorganismos desempeñan funciones esenciales y participan activamente en las cadenas tróficas y ciclos biogeoquímicos de los elementos (Pace et al., 1997; Whitman et al., 1998). A lo largo de unos 3500-4000 millones de años los microorganismos han sido capaces de colonizar todo el planeta (Madigan et al., 2000) especializándos...

  19. Influencia de una alimentación mediterránea suplementada en coenzima Q10 sobre la activación de p53 y las rutas de reparación del ADN en personas de edad avanzada

    OpenAIRE

    Gutiérrez Mariscal, Francisco Miguel

    2011-01-01

    Introducción: La hipótesis oxidativa del envejecimiento sugiere que los efectos deletéreos de las especies reactivas de oxígeno son responsables del deterioro funcional asociado con las enfermedades relacionadas con la edad (enfermedad cardiovascular, cáncer, diabetes, Alzheimer, Parkinson, etc.). La dieta Mediterránea ha demostrado ser un modelo de alimentación beneficiosa sobre la salud cardiovascular y la longevidad. La proteína p53 es la encargada de preservar la estabilidad del genoma co...

  20. Código de barras del ADN y sus posibles aplicaciones en el campo de la Entomología DNA barcoding and its possible applications to the field of Entomology

    Directory of Open Access Journals (Sweden)

    Analía A. Lanteri

    2007-12-01

    Full Text Available En este artículo se abordan algunos aspectos de la controversia sobre la iniciativa «Código de barras del ADN», y se hace hincapié en sus potenciales aplicaciones en Entomología. Esta iniciativa propone emplear información dentro de una misma región génica (gen mitocondrial de la Citocromo c Oxidasa I = COI, en todas las especies vivientes y con condiciones de secuenciación universalmente aceptadas y estandarizadas. En la actualidad, no pretende sustituir la taxonomía alfa y la filogenia sino agilizar las tareas de identificación, especialmente en el campo de la Biomedicina (identificación de patógenos, parásitos y vectores, el control de plagas (intercepción de especies invasoras, cualquiera sea su estado de desarrollo ontogenético y los estudios sobre conservación de la biodiversidad. Para arribar a una correcta delimitación de las especies biológicas es preciso contar con las secuencias de COI de numerosos individuos a lo largo de todo su rango geográfico y además, secuencias de genes nucleares e información morfológica y biológica detallada. Las «Unidades Evolutivas Significativas», identificadas sobre la base del «código de barras», podrían corresponder tanto a morfoespecies como a especies crípticas y a subespecies o linajes con diferentes preferencias de huéspedes. La integración del «código de barras del ADN», el trabajo de campo, las colecciones de museos y la investigación científica resultan imprescindibles para que esta herramienta redunde en avances significativos en el campo de la Sistemática Entomológica.This article deals with some of the most controversial issues of the DNA barcode initiative, focusing on its potential applications to Entomology. The barcoding proposes using information within the same gene region (Cytocrome c Oxidase I= COI mitochondrial gene, in all living species and under standard conditions of sequencing. At present, it does not attempt to replace alpha taxonomy or phylogeny, but to accelerate the task of identification, particularly, in the fields of Biomedicine (identification of pathogens, parasites and vectors, Pest Control (interception of all ontogenetic stages of alien invasive insects and studies on Biodiversity Conservation. For an accurate delimitation of biological species, it is necessary to undertake exhaustive sampling of COI sequences along their geographical range, as well as to sequence nuclear genes, and to accomplish detailed morphological and biological information. The «Evolutionary Significant Units» based on «DNA barcoding» might correspond to morphospecies, cryptic species, subspecies or lineages with different host preferences. The integration of «DNA barcode», field work, collections of museums and scientific research are essential for this tool to make a fruitful impact on the field of Systematic Entomology.

  1. Characterization of DNA repair phenotypes of Xeroderma pigmentosum cell lines by a paralleled in vitro test; Phenotypage de la reparation de l'ADN de lignees Xeroderma pigmentosum, par un test in vitro multiparametrique

    Energy Technology Data Exchange (ETDEWEB)

    Raffin, A.L.

    2009-06-15

    DNA is constantly damaged modifying the genetic information for which it encodes. Several cellular mechanisms as the Base Excision Repair (BER) and the Nucleotide Excision Repair (NER) allow recovering the right DNA sequence. The Xeroderma pigmentosum is a disease characterised by a deficiency in the NER pathway. The aim of this study was to propose an efficient and fast test for the diagnosis of this disease as an alternative to the currently available UDS test. DNA repair activities of XP cell lines were quantified using in vitro miniaturized and paralleled tests in order to establish DNA repair phenotypes of XPA and XPC deficient cells. The main advantage of the tests used in this study is the simultaneous measurement of excision or excision synthesis (ES) of several lesions by only one cellular extract. We showed on one hand that the relative ES of the different lesions depend strongly on the protein concentration of the nuclear extract tested. Working at high protein concentration allowed discriminating the XP phenotype versus the control one, whereas it was impossible under a certain concentration's threshold. On the other hand, while the UVB irradiation of control cells stimulated their repair activities, this effect was not observed in XP cells. This study brings new information on the XPA and XPC protein roles during BER and NER and underlines the complexity of the regulations of DNA repair processes. (author)

  2. The Improvement adn Analysis of the Asymmetric Operation with Phase Compound Excitation System%相复励励磁系统的不对称运行改善分析

    Institute of Scientific and Technical Information of China (English)

    药文忠

    2014-01-01

    For the impedance asymmetry running of excitation system with the 1FC6 generator, though the data about input and output voltage of rectifier transformer in the process of test, which were divided into the positive sequence component the negation sequence component, the vector analysis method was adopted. Through the calculation and analysis, the cause of the three phase line voltage asymmetry was fund out. By changing the connecting way of rectifier transformer, the serious asymmetry of excitation system line voltage was solved.%对1FC6发电机励磁系统的阻抗不对称运行进行分析。采用向量分析法对试验过程中整流变压器输入、输出线的电压数据进行研究,并将其分解为正序分量和负序分量。通过计算和分析找出三相线电压不对称的原因,采用改变整流变压器连接方式的方法解决励磁系统线电压严重不对称的问题。

  3. Estudio molecular preliminar de accesiones de maíz (Zea maysL. criollo e indígena Colombiano, utilizando una región de ADN cloroplástico

    Directory of Open Access Journals (Sweden)

    Ediel Armando Revelo Portilla

    2015-01-01

    Full Text Available Se exploró en forma preliminar la diversidad genética existente en las 23 razas de maíz criollo e indígena descritas para Colombia por Roberts y colaboradores (1957, para el efecto se evaluaron 28 cebadores nucleares y cloroplásticos. Catorce de ellos amplificaron en la reacción en cadena de la polimerasa (PCR y fueron enviados para secuenciar a Macrogen Inc. (Corea. Mediante programas bioinformáticos (BioEdit 7.1.0, ClustalW versión 1.81, EditPlus Text Editor versión 3.20 y Gblock 0.91b 8 se encontró que ocho de estos cebadores presentaron un nivel alto de polimorfismo. La región genómica cloroplástica AtpB-1-RbcL-1 mostró el mayor polimorfismo y por tanto se utilizó para evaluar 23 materiales representativos de las 23 razas conservadas en el Banco de Germoplasma del CIMMYT (Centro Internacional de Mejoramiento de Maíz y Trigo en México. Con el análisis de secuencias se revalidaron y confrontaron los grupos raciales obtenidos en el presente estudio -tres razas primitivas, siete razas probablemente introducidas, y 13 razas híbridas colombianas- con aquellos establecidos por Roberts y colaboradores, en los cuales se encontraron dos razas primitivas, nueve probablemente introducidas y 12 híbridas colombianas y con los grupos establecidos por Cardona (2010 utilizando la metodología Ward-MLM para los mismos caracteres descritos por Roberts et al. (1957, donde se encontraron cinco razas primitivas, siete probablemente introducidas y nueve híbridas colombianas. Con base en lo anterior, en el presente trabajo se hizo un aporte metodológico para revalidar datos históricos y redefinir grupos raciales.

  4. DSC/TG-FTIR-MS联用技术研究ADN热分解动力学和机理%Research on the kinetics and mechanism of the thermal decomposition of ADN via DSC/TG-MS-FTIR

    Institute of Scientific and Technical Information of China (English)

    王晓红; 张皋; 赵凤起; 谢明召; 任晓宁; 何少蓉

    2010-01-01

    应用DSC/TG-MS-FTIR同步热分析-红外质谱联用技术,研究了ADN的分解过程及分解机理,从ADN分解生成的气体产物的动态分布及气体产物的生成动力学方面,获得了ADN热分解的主要气体产物为N2O、NO2、NH3、H2O和N2,气体产物N2O、NO2和NH3的动力学参数的Ea值分别为157.5、158.6、100.0 kJ/mol,从微观角度提出了ADN可能的热分解机理.

  5. Detección del adn de papilomavirus humano en lesiones precancerosas y cancerosas de cuello uterino por reacción en cadena de la polimerasa, en bucaramanga, santander

    OpenAIRE

    2011-01-01

    Determinar la validez de la Reacción en Cadena de la Polimerasa (PCR) en el diagnóstico de la infección por el virus del papiloma humano (VPH), detectando diferentes tipos de VPH oncogénicos (16, 18, 31, 33 y 35) y no oncogénicos (6 y 11) en pacientes con lesiones precancerosas y cancerosas de cérvix y en pacientes control, estableciendo la prevalencia del virus en estas lesiones.

  6. El diablo en el ADN : Promiscuidad, infidelidad, violencia y violación según la sociobiología de Barash y la psicología evolucionista de Buss

    OpenAIRE

    Bargas, María Luján

    2012-01-01

    Se procura visibilizar algunas formas de determinismo biológico en torno a las diferencias comportamentales entre hombres y mujeres, que están operando al interior de dos disciplinas científicas fuertemente criticadas por las implicancias políticas que suponen sus declaraciones: la sociobiología y la psicología evolucionista. Ambas entienden que las conductas humanas están biológica y genéticamente determinadas, y que a su vez son producto de la evolución. La propuesta de este trabajo es ento...

  7. Utilisation de la PCR-RFLP sur de l'ADN chloro-plastique pour l'étude des relations phylogénétiques au sein du genre Phaseolus

    Directory of Open Access Journals (Sweden)

    Baudoin JP.

    1998-01-01

    Full Text Available Phylogenetic relationships among 74 accessions belonging to six species of Phaseolus are investigated using variation in chloroplast DNA assessed according to a PCR-RFLP protocol. Three fragments of chloroplast DNA are amplified using universal primers, and then digested with 10 restriction enzymes. Thirty-six haplotypes are identified on the basis of the polymorphism in fragment number and size. Three main phylogenetic groups, strongly supported through bootstrap analysis, are identified: (1 accessions from Phaseolus lunatus and Phaseolus xolocotzii; (2 accessions from Phaseolus glabellus; (3 accessions from Phaseolus vulgaris, Phaseolus polyanthus and Phaseolus coccineus. Within the third group, accessions of Phaseolus coccineus are scattered along the phylogenetic tree, which provides some evidence that coccineus accessions are paraphyletic with respect to Phaseolus vulgaris and Phaseolus polyanthus. An analysis of molecular variance applied on four species show that they are significantly differentiated with 79% of molecular variance among species and 21% within species. The results agree with previous investigations on chloroplast DNA variation in the genus Phaseolus, and suggest that PCRRFLP methods, which are technically less labour-intensive than previous methods, are of great value for phylogenetic investigations at the generic level.

  8. Generación de un banco de ADN de pacientes con anomalías genitourinarias y secuenciación del Exon 3 del Gen MAMLD1 en pacientes colombianos con hipospadias aisladas

    OpenAIRE

    Pabón Sogamoso, Eylin Jattin

    2014-01-01

    Las anomalías genitourinarias se manifiestan por la estrecha relación embriológica de los sistemas urinario y genital, con frecuencia afectan más a hombres y pueden exhibir un patrón familiar o una influencia ambiental. Alrededor de un tercio de las malformaciones del aparato genital se asocian a anomalías del aparato urinario. Dentro de las más frecuentes están las hipospadias las cuales se caracterizan por la presencia del orificio uretral en una región ectópica a los largo de la cara ventr...

  9. Evaluación del daño y la reparación de lesiones en el ADN inducidas por etoposido en células de fibrosarcoma mediante el ensayo del Cometa

    Directory of Open Access Journals (Sweden)

    Groot H.

    2001-06-01

    Full Text Available El estudio de las sustancias genotóxicas cada día toma un papel más importante en la vidadiaria. Numerosas sustancias genotoxicas pueden producir mutaciones en el genoma, quepueden generar procesos neoplásicos que conllevan a la formación de cáncer. En este trabajose presenta la estandarización de un micro-método para la realización de ensayos genotóxicosen cultivos celulares, utilizando células de fibrosarcoma (HT1080 cultivadas en cajas decultivo microelisa. En el proceso de estandarización, Inicialmente se realizaron recuentos paraencontrar un número de células apropiado para los ensayos.

  10. The DNA repair complex DNA-PK, a pharmacological target in cancer chemotherapy and radiotherapy; Le complexe de reparation de l'ADN DNA-PK, une cible pharmacologique en chimiotherapie et radiotherapie anticancereuse

    Energy Technology Data Exchange (ETDEWEB)

    Salles, B.; Calsou, P.; Frit, P.; Muller, C. [Institut de Pharmacologie et Biologie Structurale (IPBS), UMR CNRS 5089, 31 - Toulouse (France)

    2006-05-15

    A line of investigation in the search for sensitizing tumor cells to chemotherapy or radiotherapy relies on the selection of DNA repair inhibitors. In the area of DNA repair mechanisms, DNA-dependent protein kinase (DNA-PK) represents a key complex. Indeed DNA-PK is involved in the non-homologous end joining (NHEJ) process that corresponds to the major activity responsible for cell survival after ionizing radiation or chemotherapeutic treatment producing DNA double strand breaks. DNA-PK belongs to the PI3-K related kinase family and specific inhibitors have been recently selected and evaluated as radio- and chemo-sensitizers. These drugs, along with other ways to inhibit the DSBs repair process, are presented and discussed. (authors)

  11. DATOS POBLACIONALES DE LOS MICROSATÉLITES DE ADN HUMANO D2S1338, D19S433, PENTA D, PENTA E Y SE-33 DE LA REGIÓN CENTRAL COLOMBIANA

    Directory of Open Access Journals (Sweden)

    MAURICIO REY

    2009-01-01

    Full Text Available Los microsatélites o STR por su sigla en inglés (Short Tandem Repeat son repeticiones de secuencias cortas de nucleótidos a través del DNA, de gran importancia debido a su carácter polimórfico que permite su utilización en la identificación genética de individuos o poblacionales. En este trabajo se investigaron los perfiles genéticos de una muestra de poblaciones humanas del altiplano cundiboyacense en Colombia; se usaron los marcadores STR’s D2S1338, D19S433, PENTA D, PENTA E y SE-33. Éstos cinco STR’s fueron amplificados mediante reacción en cadena de la polimerasa (PCR, corridos con electroforesis capilar, y tipificados con los programas Genscan y Genotyper. Como resultado se reportan las frecuencias alélicas de los cinco micro- satélites (no reportados anteriormente para esta región. Se encontró que los loci D19S433 y SE-33 no están en equilibrio de Hardy-Weinberg; y que el Penta E tiene el mayor poder de discriminación. La distribución de las frecuencias alélicas para los cinco marcadores, en las dos poblaciones analizadas mostró que no existen diferencias estadísticas significativas entre ellas.

  12. DATOS POBLACIONALES DE LOS MICROSATÉLITES DE ADN HUMANO D2S1338, D19S433, PENTA D, PENTA E Y SE-33 DE LA REGIÓN CENTRAL COLOMBIANA

    Directory of Open Access Journals (Sweden)

    REY MAURICIO

    2009-08-01

    Full Text Available RESUMEN

    Los microsatélites o STR por su sigla en inglés (Short Tandem Repeat son repeticiones de secuencias cortas de nucleótidos a través del DNA, de gran importancia debido a su carácter polimórfico que permite su utilización en la identificación genética de individuos o poblacionales. En este trabajo se investigaron los perfiles genéticos de una muestra de poblaciones humanas del altiplano cundiboyacense en Colombia; se usaron los marcadores STR’s D2S1338, D19S433, PENTA D, PENTA E y SE-33. Éstos cinco STR’s fueron amplificados mediante reacción en cadena de la polimerasa (PCR, corridos con electroforesis capilar, y tipificados con los programas Genscan y Genotyper. Como resultado se reportan las frecuencias alélicas de los cinco microsatélites (no reportados anteriormente para esta región. Se encontró que los loci D19S433 y SE-33 no están en equilibrio de Hardy-Weinberg; y que el Penta E tiene el mayor poder de discriminación. La distribución de las frecuencias alélicas para los cinco marcadores, en las dos poblaciones analizadas mostró que no existen diferencias estadísticas significativas entre ellas.

    Palabras clave: STR, D2S1338, D19S433, PENTA D, PENTA E, SE-33.

    ABSTRACT

    Microsatellites (STR’s are short tandem repeat sequences of nucleotides through DNA, very important for their polymorphic character that allow their utilization in population genetic identification. The STR’s markers D2S1338, D19S433, PENTA D, PENTA E y SE- 33 were used to do the genetic profiles of human populations from the Cundiboyacense region in Colombia. The five STR’s were amplified by PCR (polymerase chain reaction, indentified with capillary electrophoresis, and typified with Genscan and Genotyper software. Allelic frequencies of the five microsatellites are reported (not previously reported for this region. It was found that loci D19S433 y SE-33 are not in Hardy- Weinberg equilibrium; and Penta E provides the greatest power of discrimination. The allelic frequencies distribution to these five markers, in the two populations studied show that it does not exist statistically significant differences between them.

    Key words: STR, D2S1338, D19S433, PENTA D, PENTA E, SE-33.

  13. Impact de l'organisation du noyau et de la structure de la chromatine sur la réparation de l'ADN et la stabilité du génome

    OpenAIRE

    Batté, Amandine

    2016-01-01

    The non-random organization of the eukaryotic cell nucleus and the folding of genome in chromatin more or less condensed can influence many functions related to DNA metabolism, including genome stability. Double-strand breaks (DSBs) are the most deleterious DNA damages for the cells. To preserve genome integrity, eukaryotic cells thus developed DSB repair mechanisms conserved from yeast to human, among which homologous recombination (HR) that uses an intact homologous sequence to repair a bro...

  14. Catálogo de las muestras de fauna de la Comunidad de Madrid conservadas en la colección de Tejidos y ADN del Museo Nacional de Ciencias Naturales

    OpenAIRE

    Rey, I; Dorda, B. A.

    2006-01-01

    This work presents the catalogue of preserved samples of animal species (vertebrate and invertebrate) from Comunidad de Madrid kept in the Tissues and DNA Collection of the Museo Nacional de Ciencias Naturales. Percentages are shown, calculated against the total number of samples and animal class kept in the collection. This information shows the effort developed by the Museo Nacional de Ciencias Naturales to preserve at the genomic level the greatest possible level of genomic biodiversity in...

  15. Detección del ADN de Papilomavirus humano en lesiones precancerosas y cancerosas de cuello uterino por reacción en cadena de la polimerasa, en Bucaramanga, Santander

    Directory of Open Access Journals (Sweden)

    Castillo A.

    2001-06-01

    Full Text Available Determinar la validez de la Reacción en Cadena de la Polimerasa (PCR en el diagnóstico de la infección por el virus del papiloma humano (VPH, detectando diferentes tipos de VPH oncogénicos (16, 18, 31, 33 y 35 y no oncogénicos (6 y 11 en pacientes con lesiones precancerosas y cancerosas de cérvix y en pacientes control, estableciendo la prevalencia del virus en estas lesiones.

  16. DNA breaks and repair in interstitial telomere sequences: Influence of chromatin structure; Etude des cassures de l'ADN et des mecanismes de reparation dans les sequences telomeriques interstitielles: Influence de la structure chromatinienne

    Energy Technology Data Exchange (ETDEWEB)

    Revaud, D.

    2009-06-15

    Interstitial Telomeric Sequences (ITS) are over-involved in spontaneous and radiationinduced chromosome aberrations in chinese hamster cells. We have performed a study to investigate the origin of their instability, spontaneously or after low doses irradiation. Our results demonstrate that ITS have a particular chromatin structure: short nucleotide repeat length, less compaction of the 30 nm chromatin fiber, presence of G-quadruplex structures. These features would modulate breaks production and would favour the recruitment of alternative DNA repair mechanisms, which are prone to produce chromosome aberrations. These pathways could be at the origin of chromosome aberrations in ITS whereas NHEJ and HR Double Strand Break repair pathways are rather required for a correct repair in these regions. (author)

  17. Relationship of the demethylation of the DNA with the induction of the sister chromatid exchanges (SCE) In vivo; Relacion de la desmetilacion del ADN con la induccion de intercambios en las cromatidas hermanas (ICH) In vivo

    Energy Technology Data Exchange (ETDEWEB)

    Toribio E, E

    2005-07-01

    The methylation of the DNA is an epigenetic modification that has an important paper in the regulation of the functionality of the genome of the organisms. It can be altered by demethylation processes, either natural or experimentally induced. The 5-azacytidine (Aza) is a compound that causes the demethylation of the DNA (dm-DNA), inducing with it, expression genic and increase in the frequency of the Sister Chromatid Exchange (SCE). The SCE is a genotoxicity indicator, caused by diverse mutagens and carcinogen. Since the biological meaning and the formation mechanism of this phenomenon has not been totally illustrious, the exploration of the relation between the dm-DNA and the induction of SCE, it could offer new knowledge to explain those queries. The purpose of this work was to study in cells of the mouse bone marrow In vivo, the effect of the Aza on the induction of SCE, based on two aspects: 1) dose answer and 2) the effectiveness of multiple exhibition. To six groups of three to five animals, they are administered Aza to dose of 5, 10, 15 or 20 mg/Kg of weight; in sharp or multiple form, previously to the bromodeoxyuridine supply and 24 h was sacrificed after this; 2 h after an injection with colchicine. Preparations of those metaphases were made, those which were dyed by means of a technique of fluorescence more Giemsa. It was observed that to sharp low dose, the Aza produced an increment in the frequency of SCE that although small it was proportional and statistically significant. To sharp and multiple high doses, the Aza doesn't cause additional increments of SCE, but if toxicity at cellular level and of individuals. It is concluded that a relationship exists between the dm-DNA and the induction of SCE. It is suggested that the total demethylation of the DNA causes 2 SCE/Cell in cells of the mouse bone marrow, or that the cytotoxicity prevents to evidence a bigger induction. (Author)

  18. Taxonomía a golpe de proteínas y ADN mitocondrial: el caso de la Orça - Developing taxonomy using proteins and mitocondrial DNA: The case of the Orca

    Directory of Open Access Journals (Sweden)

    Arija, Carmen M.

    2011-02-01

    Full Text Available ResumenLos estudios moleculares han dado un vuelco a la Taxonomía,erigiéndose como una herramienta imprescindible para la determinación de los límites de una especie o un grupo y su situación en el complejo árbol de los seres vivos.SummaryMolecular studies have overturned the taxonomy, establishing itself asan indispensable tool for determining the limits of an specie or a group, and their situation in the Living tree.

  19. Determination of damage and In vivo DNA repairing through the unicellular in gel electrophoresis technique; Determinacion del dano y la reparacion del ADN In vivo mediante la tecnica de electroforesis unicelular en gel

    Energy Technology Data Exchange (ETDEWEB)

    Mendiola C, M.T.; Morales R, P. [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1997-07-01

    The experimental conditions were standardized for the unicellular in gel electrophoresis technique setting up (EUG) at the Cellular Radiobiology laboratory. Preliminary experiments were realized with human cells and mouse which were exposed to ionizing radiation or hydroxide peroxide (H{sub 2}O{sub 2}) to induce DNA damage and to verify the technique performance. It was analysed the In vivo repairing kinetics of induced damage by gamma radiation in mouse leukocytes which were exposed to {sup 137} Cs source and taking samples of peripheric blood of the tail of each mouse at different exposure times and processing them for EUG. In function of the cells proportion with damage in each time it was determined the existence of fast repairing mechanism at the first 15 minutes followed by a slight increase in the damage and a late repairing stage between 30 and 90 minutes. It was analysed this behavior and the potentiality of this In vivo system. (Author)

  20. Estudio molecular preliminar de accesiones de maíz (zea mays l.) criollo e indígena colombiano utilizando una región de adn cloroplástico

    OpenAIRE

    2014-01-01

    Se exploró se forma preliminar la diversidad genética existente en las 23 razas de maíz criollo e indígena descritas para Colombia por Roberts y colaboradores (1957), para el efecto se evaluaron 28 cebadores nucleares y cloroplásticos. Catorce de ellos amplificaron en la reacción en cadena de la polimerasa (PCR) y fueron enviados para secuenciar a Macrogen Inc. (Corea). Mediante programas bioinformáticos (BioEdit 7.1.0, ClustalW versión 1.81, EditPlus Text Editor versión 3.20 y Gblock 0.91b...

  1. Estudio molecular preliminar de accesiones de maíz (Zea maysL.) criollo e indígena Colombiano, utilizando una región de ADN cloroplástico

    OpenAIRE

    2015-01-01

    Se exploró en forma preliminar la diversidad genética existente en las 23 razas de maíz criollo e indígena descritas para Colombia por Roberts y colaboradores (1957), para el efecto se evaluaron 28 cebadores nucleares y cloroplásticos. Catorce de ellos amplificaron en la reacción en cadena de la polimerasa (PCR) y fueron enviados para secuenciar a Macrogen Inc. (Corea). Mediante programas bioinformáticos (BioEdit 7.1.0, ClustalW versión 1.81, EditPlus Text Editor versión 3.20 y Gblock 0.91b 8...

  2. Detección de secuencias específicas de ADN de Spongospora subterranea en suelo y tubérculos de papa Detection of DNA specific sequences of Spongospora subterranea in soil and potato tubers

    OpenAIRE

    Ángel Díaz Jorge Evelio; Gómez González Sandra Janeth; Saavedra Rodríguez Cristian Oswaldo

    2004-01-01

    Con el fin de identificar de manera precoz el agente causal de la sarna polvosa de la papa (Spongospora subterranea fs subterranea) tanto en semillas como en suelos aptos para el cultivo de este tubérculo, se ha desarrollado una prueba basada en la reacción en cadena de la polimerasa (PCR) para la detección de tres secuencias ITS (espaciadores de transcripción interna específicos del ADNr) de 372,390 y 391 pb presentes en el genoma de S. subterranea. Para ello se estandarizó una metodología d...

  3. Evaluation of a radioimmunoassay for the determination of anti-native DNA antibodies. Evaluation d'une technique radio-immunologique pour la detection des anticorps anti-ADN natif

    Energy Technology Data Exchange (ETDEWEB)

    Johanet, C.; Soulie, E.; Absalon, Y.B.; Ocwieja, T.; Abuaf, N. (Hopital Saint-Antoine, 75 - Paris (FR))

    1991-01-01

    The anti-native DNA antibodies were measured by a radioimmunoassay (RIA) type Farr assay in the sera from 648 patients: 108 with active or inactive systemic lupus erythematosus (SLE), 181 with clinical symptoms of another connective tissue disease, 171 with liver diseases, 29 with different pathology and 159 normal sera were obtained from a blood bank. The anti-DNA kit has been calibrated against the first international units/ml. This assay has proved to be sensitive and specific, and appears to be reliable for the diagnosis and follow-up of SLE patients. The authors propose a new reference cut-off level higher than producer's one.

  4. A Study of the IEEE 802.16 MAC Layer and its Utility in Augmenting the ADNS Architecture to Provide Adaptable Intra-Strike Group High-Speed Packet Switched Data, Imagery, and Voice Communications

    Science.gov (United States)

    2005-09-01

    Johnson Jameau R Pryor Approved by: Rex Buddenberg Thesis Co-Advisor John Gibson Thesis Co-Advisor Dan Boger Chairman...C. NETWORK ENTRY .....................................................................................16 1. Scan for DL Channel and Establish...Sublayer CSG Carrier Strike Group DHCP Dynamic Host Configuration Protocol DL Downlink DNS Domain Name Server DOD Department of Defense

  5. The results of the lipids peroxidation products on the DNA bases as biological markers of the oxidative stress; Les adduits des produits de la peroxydation lipidique sur les bases de l'ADN comme biomarqueurs du stress oxydant

    Energy Technology Data Exchange (ETDEWEB)

    Falletti, O

    2007-10-15

    Different ways of DNA damages have been studied, among these ones the direct way of DNA damages formation by the reactive oxygen species (R.O.S.). This way leads to the formation of oxidative DNA damages. In 1990, works have suggested an indirect way of DNA damages formation, the lipids peroxidation. Instead of oxidizing directly DNA, the R.O.S. oxide the lipids present in the cells and their membranes; The products coming from this degradation are able to provoke DNA damages. This way has not been studied very much. The work of this thesis is axed on this DNA theme and lipids peroxidation. In the first chapter, we begin by presenting DNA and the direct way of oxidative damages formation by the R.O.S.Then, we speak about the cell lipids suffering oxidation reactions and the different ways of lipids oxidation. Then, we present how the lipid peroxidation is a source of damages for DNA. (N.C.)

  6. Physical exercise-induced hippocampal neurogenesis and antidepressant effects are mediated by the adipocyte hormone adiponectin.

    Science.gov (United States)

    Yau, Suk Yu; Li, Ang; Hoo, Ruby L C; Ching, Yick Pang; Christie, Brian R; Lee, Tatia M C; Xu, Aimin; So, Kwok-Fai

    2014-11-04

    Adiponectin (ADN) is an adipocyte-secreted protein with insulin-sensitizing, antidiabetic, antiinflammatory, and antiatherogenic properties. Evidence is also accumulating that ADN has neuroprotective activities, yet the underlying mechanism remains elusive. Here we show that ADN could pass through the blood-brain barrier, and elevating its levels in the brain increased cell proliferation and decreased depression-like behaviors. ADN deficiency did not reduce the basal hippocampal neurogenesis or neuronal differentiation but diminished the effectiveness of exercise in increasing hippocampal neurogenesis. Furthermore, exercise-induced reduction in depression-like behaviors was abrogated in ADN-deficient mice, and this impairment in ADN-deficient mice was accompanied by defective running-induced phosphorylation of AMP-activated protein kinase (AMPK) in the hippocampal tissue. In vitro analyses indicated that ADN itself could increase cell proliferation of both hippocampal progenitor cells and Neuro2a neuroblastoma cells. The neurogenic effects of ADN were mediated by the ADN receptor 1 (ADNR1), because siRNA targeting ADNR1, but not ADNR2, inhibited the capacity of ADN to enhance cell proliferation. These data suggest that adiponectin may play a significant role in mediating the effects of exercise on hippocampal neurogenesis and depression, possibly by activation of the ADNR1/AMPK signaling pathways, and also raise the possibility that adiponectin and its agonists may represent a promising therapeutic treatment for depression.

  7. On the Explicit Determination of the Chapman-Jouguet Parameters for an Explosive Compound

    Science.gov (United States)

    2014-11-19

    AMATOL, ADN , CYCLOTOL 75/25 and PBX 9502 which is considered to be a heterogeneous explosive compound. For these materials there were some...Dinitramide ( ADN ), Central European Journal of Energetic Materials, 8(2); 99-106. [18] Muthurajan, H., Sivahalan, R., Talawar, M.B., and Asthama, S.N...and Östmark, H., (1998) Sensitivity and Performance Characterization of Ammonium Dinitramide ( ADN ), Eleventh International Detonation Symposium

  8. Role Preparation of Associate Degree Graduates.

    Science.gov (United States)

    Stokes, Lillian G.

    The role of associate degree nursing (ADN) programs has changed dramatically in their 30 years of existence. The number of ADN graduates increased from 260 in 1954 to 36,434 in 1980, and 47.8% of all nursing graduates in 1980 came from ADN programs, as compared to 0.9% in 1954. These graduates have the best record of employment five years after…

  9. The Effect of Variable Composition Equilibrium Thermochemistry in Constant Breech Pressure (CBP) Gun Simulations

    Science.gov (United States)

    2014-01-01

    Systems. This category includes several systems using GAP/ ADN and GAP/CL20 as propellant [13]. RESULTS AND DISCUSSION Calculations were performed...high impetus (ca. 1,300 and 1,400 J/g) GAP- ADN -CL20 propellant [13]. Nominal results were obtained for this series of six configurations except for the...systems. ACKNOWLEDGMENTS The author is indebted to Dr. Kevin White for helpful discussions, to Dr. Arpad Juhasz for information on the GAP- ADN -CL20

  10. Novel immunomodulatory effects of adiponectin on dendritic cell functions.

    Science.gov (United States)

    Tsang, Julia Yuen Shan; Li, Daxu; Ho, Derek; Peng, Jiao; Xu, Aimin; Lamb, Jonathan; Chen, Yan; Tam, Paul Kwong Hang

    2011-05-01

    Adiponectin (ADN) is an adipocytokine with anti-inflammatory properties. Although it has been reported that ADN can inhibit the immunostimulatory function of monocytes and macrophages, little is known of its effect on dendritic cells (DC). Recent data suggest that ADN can regulate immune responses. DCs are uniquely specialised antigen presenting cells that play a central role in the initiation of immunity and tolerance. In this study, we have investigated the immuno- modulatory effects of ADN on DC functions. We found that ADN has only moderate effect on the differentiation of murine bone marrow (BM) derived DCs but altered the phenotype of DCs. The expression of major histocompatibilty complex class II (MHCII), CD80 and CD86 on ADN conditioned DCs (ADN-DCs) was lower than that on untreated cells. The production of IL-12p40 was also suppressed in ADN-DCs. Interestingly, ADN treated DCs showed an increase in the expression of the inhibitory molecule, programmed death-1 ligand (PDL-1) compared to untreated cells. In vitro co-culture of ADN-DCs with allogeneic T cells led to a decrease in T cell proliferation and reduction of IL-2 production. Concomitant with that, a higher percentage of CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) was detected in co-cultures of T cells and ADN-DCs. Blocking PD-1/PDL-1 pathway could partially restore T cell function. These findings suggest that the immunomodulatory effect of ADN on immune responses could be at least partially be mediated by its ability to alter DC function. The PD-1/PDL-1 pathway and the enhancement of Treg expansion are implicated in the immunomodulatory mechanisms.

  11. CrossTalk: The Journal of Defense Software Engineering. Volume 26, Number 3, May-June 2013

    Science.gov (United States)

    2013-06-01

    University Gauthier Fanmuy, ADN Abstract. Very Small Entities (VSEs) developing systems or software are very important to the military since the...Dublin, Ireland E-mail: roconnor@computing.dcu.ie Gauthier Fanmuy is a Department Director at ADN <http://www.adneurope.com>, a Systems Engineering...Engineering, <http:www.afis.fr> and AFIS representative at AFNOR. He is Associ- ate Technical Director for Industry in INCOSE. ADN Systems Engineering

  12. 二硝酰胺铵与异氰酸酯化合物的红外光谱研究%The Study on FTIR Spectra of Ammonium Dinitramide with Compounds of Isocyanate

    Institute of Scientific and Technical Information of China (English)

    翟进贤; 杨荣杰; 李建民

    2006-01-01

    通过将ADN、ADN/N-100、ADN/TDI、ADN/IPDI分别在室温和45 ℃下处理后进行FTIR谱图分析,研究了二硝酰胺铵(ADN)与三种异氰酸酯化合物(TDI、N-100、IPDI)的相容性.研究表明,在室温和45 ℃下,ADN与N-100、TDI不相容而与IPDI相容.

  13. Association between reduction of plasma adiponectin levels and risk of bacterial infection after gastric cancer surgery.

    Directory of Open Access Journals (Sweden)

    Hiroshi Yamamoto

    Full Text Available BACKGROUND AND PURPOSE: Infections are important causes of postoperative morbidity after gastric surgery; currently, no factors have been identified that can predict postoperative infection. Adiponectin (ADN mediates energy metabolism and functions as an immunomodulator. Perioperative ADN levels and perioperative immune functioning could be mutually related. Here we evaluated a potential biological marker to reliably predict the incidence of postoperative infections to prevent such comorbidities. METHODS: We analyzed 150 consecutive patients who underwent elective gastric cancer surgery at the Shiga University of Medical Science Hospital (Shiga, Japan from 1997 to 2009; of these, most surgeries (n = 100 were performed 2008 onwards. The patient characteristics and surgery-related factors between two groups (with and without infection were compared by the paired t-test and χ(2 test, including preoperative ADN levels, postoperative day 1 ADN levels, and ADN ratio (postoperative ADN levels/preoperative ADN levels as baseline factors. Logistic regression analysis was performed to access the independent association between ADN ratio and postoperative infection. Finally, receiver operating curves (ROCs were constructed to examine its clinical utility. RESULTS: Sixty patients (40% experienced postoperative infections. The baseline values of age, American Society of Anesthesiologists physical status, total operating time, blood loss, surgical procedure, C-reactive protein (CRP levels, preoperative ADN levels, and ADN ratio were significantly different between groups. Logistic regression analysis using these factors indicated that type 2 diabetes mellitus (T2DM and ADN ratio were significantly independent variables (*p<0.05, ** p<0.01, respectively. ROC analysis revealed that the useful cutoff values (sensitivity/specificity for preoperative ADN levels, ADN ratio, blood loss, operating time, and CRP levels were 8.81(0.567/0.568, 0.76 (0

  14. Plasma adiponectin levels are increased despite insulin resistance in corticotropin-releasing hormone transgenic mice, an animal model of Cushing syndrome.

    Science.gov (United States)

    Shinahara, Masayuki; Nishiyama, Mitsuru; Iwasaki, Yasumasa; Nakayama, Shuichi; Noguchi, Toru; Kambayashi, Machiko; Okada, Yasushi; Tsuda, Masayuki; Stenzel-Poore, Mary P; Hashimoto, Kozo; Terada, Yoshio

    2009-01-01

    Adiponectin (AdN), an adipokine derived from the adipose tissue, has an insulin-sensitizing effect, and plasma AdN is shown to be decreased in obesity and/or insulin resistant state. To clarify whether changes in AdN are also responsible for the development of glucocorticoid-induced insulin resistance, we examined AdN concentration in plasma and AdN expression in the adipose tissue, using corticotropin-releasing hormone (CRH) transgenic mouse (CRH-Tg), an animal model of Cushing syndrome. We found, unexpectedly, that plasma AdN levels in CRHTg were significantly higher than those in wild-type littermates (wild-type: 19.7+/-2.5, CRH-Tg: 32.4+/-3.1 microg/mL, pAdN mRNA and protein levels were significantly decreased in the adipose tissue of CRH-Tg. Bilateral adrenalectomy in CRH-Tg eliminated both their Cushing's phenotype and their increase in plasma AdN levels (wild-type/sham: 9.4+/-0.5, CRH-Tg/sham: 15.7+/-2.0, CRH-Tg/ADX: 8.5+/-0.4 microg/mL). These results strongly suggest that AdN is not a major factor responsible for the development of insulin resistance in Cushing syndrome. Our data also suggest that glucocorticoid increases plasma AdN levels but decreases AdN expression in adipocytes, the latter being explained possibly by the decrease in AdN metabolism in the Cushing state.

  15. Selected Bibliography on Associate Degree Programs in Nursing.

    Science.gov (United States)

    National League for Nursing, New York, NY. Dept. of Associate Degree Programs.

    This annotated bibliography consists of 99 entries, primarily journal articles, most of which were published after 1966. Organization is under the following headings: (1) Associate Degree Nursing (ADN) Programs--What They Are, (2) Planning for ADN Program, (3) Move to Educational Institutions, (4) Administration, (5) Faculty, (6) Teaching Methods,…

  16. Review of Solid Propellant Ignition Models Relative to the Interior Ballistic Modelling of Gun Systems

    Science.gov (United States)

    2012-08-01

    the point of phase change is reached. Solid-phase exothermic reactions may occur in some propellant ingredients (such as AP [9] or ADN [4]) leading...M.L. Gross. Two-dimensional modeling of AP/HTPB utilizing a vorticity formula- tion and one-dimensional modeling of AP and ADN . PhD thesis, Brigham

  17. Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modeling.

    Science.gov (United States)

    Correnti, Jason M; Cook, Daniel; Aksamitiene, Edita; Swarup, Aditi; Ogunnaike, Babatunde; Vadigepalli, Rajanikanth; Hoek, Jan B

    2014-11-10

    Following partial hepatectomy, the liver initiates a regenerative program involving hepatocyte priming and replication driven by coordinated cytokine and growth factor actions. We investigated the mechanisms underlying Adiponectin's (Adn) regulation of liver regeneration through modulation of these mediators. Adn-/- mice showed delayed onset of hepatocyte replication, but accelerated cell cycle progression relative to wild-type mice, suggesting Adn has multiple effects fine-tuning the kinetics of liver regeneration. We developed a computational model describing the molecular and physiological kinetics of liver regeneration in Adn-/- mice. We employed this computational model to evaluate the underlying regulatory mechanisms. Our analysis predicted that Adn is required for an efficient early cytokine response to partial hepatectomy, but is inhibitory to later growth factor actions. Consistent with this prediction, Adn knockout reduced hepatocyte responses to IL-6 during the priming phase, but enhanced growth factor levels through peak hepatocyte replication. By contrast, supraphysiological concentrations of Adn resulting from rosiglitazone treatment suppressed regeneration by reducing growth factor levels during S phase, consistent with computational predictions. Together, these results revealed that Adn fine-tunes the progression of liver regeneration through dynamically modulating molecular mediator networks and cellular interactions within the liver. This article is protected by copyright. All rights reserved.

  18. Current Issues Related to Associate Degree Nursing: Their Impact on Directors, Faculty, and Students.

    Science.gov (United States)

    Cavenar, Mary G.

    Perceptions of students, faculty members, and associate degree nursing (ADN) program directors/coordinators concerning seven issues affecting ADN programs were obtained in 1982. The issues, which were identified through a review of the literature, were: entry into practice, professional and technical nurses, developing competency statements,…

  19. Associate Degree Nursing Program Guide. Final Report from February 19, 1985 to August 31, 1985.

    Science.gov (United States)

    Seminole Community Coll., Sanford, FL.

    This program guide is intended to help Associate Degree Nursing (ADN) instructors in Florida develop and/or update ADN programs. The first part is the final report of the project that developed the guide. Section I of the guide provides a description of the occupation, student admission criteria, retention and withdrawal standards, and program…

  20. Registered Nurse Education and the Registered Nurse Job Market.

    Science.gov (United States)

    Hunt, Howard Allan

    This effort compares the graduates of the three types of Registered Nurse (RN) education programs (three-year Diploma in Nursing, two-year Associate Degree in Nursing (ADN), and four-year Bachelor of Science Degree in Nursing). The basic objective is to determine whether they are perfect substitutes, especially whether ADN graduates can adequately…

  1. The Associate Degree Nursing Program at Rio Hondo College: A Research Report.

    Science.gov (United States)

    Michaels, Joseph

    During 1975-76, an evaluation of the Associate Degree Nursing (ADN) program at Rio Hondo College was undertaken which involved: (1) surveying all nursing graduates in the classes of 1973, 1974, and 1975, and all fourth semester students currently enrolled in the program; (2) surveying or interviewing all instructional staff for the ADN program;…

  2. Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modelling.

    Science.gov (United States)

    Correnti, Jason M; Cook, Daniel; Aksamitiene, Edita; Swarup, Aditi; Ogunnaike, Babatunde; Vadigepalli, Rajanikanth; Hoek, Jan B

    2015-01-15

    Following partial hepatectomy, the liver initiates a regenerative programme involving hepatocyte priming and replication driven by the coordinated actions of cytokine and growth factors. We investigated the mechanisms underlying adiponectin's (Adn) regulation of liver regeneration through modulation of these mediators. Adn(-/-) mice showed delayed onset of hepatocyte replication, but accelerated cell cycle progression relative to wild-type mice, suggesting Adn has multiple effects fine-tuning the kinetics of liver regeneration. We developed a computational model describing the molecular and physiological kinetics of liver regeneration in Adn(-/-) mice. We employed this computational model to evaluate the underlying regulatory mechanisms. Our analysis predicted that Adn is required for an efficient early cytokine response to partial hepatectomy, but is inhibitory to later growth factor actions. Consistent with this prediction, Adn knockout reduced hepatocyte responses to interleukin-6 during the priming phase, but enhanced growth factor levels through peak hepatocyte replication. By contrast, supraphysiological concentrations of Adn resulting from rosiglitazone treatment suppressed regeneration by reducing growth factor levels during S phase, consistent with computational predictions. Together, these results revealed that Adn fine-tunes the progression of liver regeneration through dynamically modulating molecular mediator networks and cellular interactions within the liver.

  3. Nursing Transition: An Individualized Course To Promote Mobility from the LVN to RN Role. Registered Nurse Shortage Project. Final Report.

    Science.gov (United States)

    Irwin, Marcia; Malague, Marianne

    To address a regional shortage of registered nurses (RN's), a special transition course was developed at the North Harris Montgomery Community College District in Houston, Texas, to allow licensed vocational nurses to articulate into the second year of a two-year Associate Degree Nursing (ADN) program. Students completing the ADN program are…

  4. Predictors of NCLEX-RN Success of Associate Degree Graduates: A Correlational Study

    Science.gov (United States)

    Kehm, Bonny J.

    2013-01-01

    The outcome of Associate Degree Nursing (ADN) students not passing the initial National Council of Licensure Examination for Registered Nursing (NCLEX-RN) can adversely affect schools of nursing. This failure also adversely affects the national nursing shortage. The declining national pass rates on the NCLEX-RN for ADN graduates and the increasing…

  5. The Origins and Rise of Associate Degree Nursing Education.

    Science.gov (United States)

    Haase, Patricia T.

    This book offers an analytical history of the associate degree nursing (ADN) program and the role of associate degree nurses in the U.S. health care system. It covers the period from just after World War II to the middle of the 1980s. The support of the W. K. Kellogg Foundation and the Federal Government for ADN education is highlighted. Chapter…

  6. Issues in Associate Degree Nursing. Bridge to Success. Education and Service: A Partnership for Associate Degree Nursing.

    Science.gov (United States)

    Southern Regional Education Board, Atlanta, GA.

    This publication is a compilation of highlights from papers presented at the Associate Degree Nursing (ADN) project's regional conferences during 1983-84. Papers address pertinent issues in ADN education and practice. "AD Education: Are the Parameters Real?" (Julia Perkins) examines the parameters of associate degree nursing education from a…

  7. The Past, the Present, and the Future of Associate Degree Nursing Education.

    Science.gov (United States)

    Arlton, Donna

    A review of the history of associate degree nursing (ADN) education is presented, along with a discussion of contemporary problems faced by ADN educators. The paper first notes the practical, hospital-based nature of early nursing education programs; reviews early studies calling for school-based programs to prepare nurses for different levels of…

  8. Boundary Spanners as supports of social capital

    DEFF Research Database (Denmark)

    Vincenti, Gordon

    2016-01-01

    Boundary Spanners are important agenets of supporting the capacity building of local neighbourhoods and of sustainable social captial the article focuses on the skills and competnces adn role of Boundary Spanners.......Boundary Spanners are important agenets of supporting the capacity building of local neighbourhoods and of sustainable social captial the article focuses on the skills and competnces adn role of Boundary Spanners....

  9. Effects of atmospheric deposition nitrogen flux and its composition on soil solution chemistry from a red soil farmland, southeast China.

    Science.gov (United States)

    Cui, Jian; Zhou, Jing; Peng, Ying; Chan, Andrew; Mao, Jingdong

    2015-12-01

    A detailed study on the solution chemistry of red soil in South China is presented. Data are collected from two simulated column-leaching experiments with an improved setup to evaluate the effects of atmospheric N deposition (ADN) composition and ADN flux on agricultural soil acidification using a (15)N tracer technique and an in situ soil solution sampler. The results show that solution pH values decline regardless of the increase of the NH4(+)/NO3(-) ratio in the ADN composition or ADN flux, while exchangeable Al(3+), Ca(2+), Mg(2+), and K(+) concentrations increase at different soil depths (20, 40, and 60 cm). Compared with the control, ADN (60 kg per ha per year N, NH4(+)/NO3(-) ratio of 2 : 1) decreases solution pH values, increases solution concentrations of NO3(-)-N, Al(3+), Ca(2+) and Mg(2+) at the middle and lower soil depths, and promotes their removal. NH4(+)-N was not detected in red soil solutions of all the three soil layers, which might be attributed to effects of nitrification, absorption and fixation in farmland red soil. Some of the NO3(-)-N concentrations at 40-60 cm soil depth exceed the safe drinking level of 10 mg L(-1), especially when the ADN flux is beyond 60 kg ha(-1) N. These features are critical for understanding the ADN agro-ecological effects, and for future assessment of ecological critical loads of ADN in red soil farmlands.

  10. Aqueous dispersions of oxide nanoparticles as a treatment for pyoinflammatory diseases with chronic component

    Energy Technology Data Exchange (ETDEWEB)

    Rutberg, Ph; Kolikov, V; Snetov, V; Stogov, A [Institute for Electrophysics and Electric Power Russian Academy of Sciences, 18 Dvortsovaya nab., St.-Petersburg, 191186 (Russian Federation); Moshkin, A; Khalilov, M, E-mail: Stogov2007@yandex.ru [Oryol State University, Medical Institute, October st. 25, Oryol, 302028 (Russian Federation)

    2011-04-01

    Promising direction of surgery related to the treatment of acute purulent wounds with chronic component could be utilization of aqueous dispersions of nanostructures (ADN) produced by pulsed electric discharge in water. The investigation is addressed to finding out the opportunity of usage of an ADN for treatment of purulent wounds with a chronic component and comparison of its efficiency with the widespread antiseptics. For realization of investigation was used ADN, which has maximal share of 'small' nanostructures (<100 nm) with the greatest surface electric charge. High activity of reparative processes is established at use of ADN and subsequent moderate changes of the further healing. The attributes of cellular atypia and preternatural representations about inflammatory reactions are not revealed at local use of ADN.

  11. Globular adiponectin controls insulin-mediated vasoreactivity in muscle through AMPKα2

    DEFF Research Database (Denmark)

    de Boer, Michiel P; Meijer, Rick I; Richter, Erik

    2016-01-01

    Decreased tissue perfusion increases the risk of developing insulin resistance and cardiovascular disease in obesity, and decreased levels of globular adiponectin (gAdn) have been proposed to contribute to this risk. We hypothesized that gAdn controls insulin's vasoactive effects through AMP......-activated protein kinase (AMPK), specifically its α2 subunit, and studied the mechanisms involved. In healthy volunteers, we found that decreased plasma gAdn levels in obese subjects associate with insulin resistance and reduced capillary perfusion during hyperinsulinemia. In cultured human microvascular...... endothelial cells (HMEC), gAdn increased AMPK activity. In isolated muscle resistance arteries gAdn uncovered insulin-induced vasodilation by selectively inhibiting insulin-induced activation of ERK1/2, and the AMPK inhibitor compound C as well as genetic deletion of AMPKα2 blunted insulin...

  12. Adiponectin stimulates Wnt inhibitory factor-1 expression through epigenetic regulations involving the transcription factor specificity protein 1.

    Science.gov (United States)

    Liu, Jing; Lam, Janice B B; Chow, Kim H M; Xu, Aimin; Lam, Karen S L; Moon, Randall T; Wang, Yu

    2008-11-01

    Adiponectin (ADN) is an adipokine possessing growth inhibitory activities against various types of cancer cells. Our previous results demonstrated that ADN could impede Wnt/beta-catenin-signaling pathways in MDA-MB-231 human breast carcinoma cells [Wang,Y. et al. (2006) Adiponectin modulates the glycogen synthase kinase-3 beta/beta-catenin signaling pathway and attenuates mammary tumorigenesis of MDA-MB-231 cells in nude mice. Cancer Res., 66, 11462-11470]. Here, we extended our studies to elucidate the effects of ADN on regulating the expressions of Wnt inhibitory factor-1 (WIF1), a Wnt antagonist frequently silenced in human breast tumors. Our results showed that ADN time dependently stimulated WIF1 gene and protein expressions in MDA-MB-231 cells. Overexpression of WIF1 exerted similar inhibitory effects to those of ADN on cell proliferations, nuclear beta-catenin activities, cyclin D1 expressions and serum-induced phosphorylations of Akt and glycogen synthase kinase-3 beta. Blockage of WIF1 activities significantly attenuated the suppressive effects of ADN on MDA-MB-231 cell growth. Furthermore, our in vivo studies showed that both supplementation of recombinant ADN and adenovirus-mediated overexpression of this adipokine substantially enhanced WIF1 expressions in MDA-MB-231 tumors implanted in nude mice. More interestingly, we found that ADN could alleviate methylation of CpG islands located within the proximal promoter region of WIF1, possibly involving the specificity protein 1 (Sp1) transcription factor and its downstream target DNA methyltransferase 1 (DNMT1). Upon ADN treatment, the protein levels of both Sp1 and DNMT1 were significantly decreased. Using silencing RNA approaches, we confirmed that downregulation of Sp1 resulted in an increased expression of WIF1 and decreased methylation of WIF1 promoter. Taken together, these data suggest that ADN might elicit its antitumor activities at least partially through promoting WIF1 expressions.

  13. Combustion Characteristics and Thermal Decomposition Behavior of Ammonium Dinitramide Catalyzed by Lead Carbonate%碳酸铅催化二硝酰胺铵的燃烧特性和热分解行为研究

    Institute of Scientific and Technical Information of China (English)

    翟进贤; 杨荣杰; 李建民; 李晓东

    2005-01-01

    通过燃速测定、差示扫描量热法(DSC)和热失重法(TG)研究了碳酸铅(PbCO3)对二硝酰胺铵(ADN)热分解和燃烧性能的影响.在3~12 MPa范围内,ADN+5% PbCO3+0.2%石蜡的燃速高于ADN+0.2%石蜡,PbCO3使ADN+0.2%石蜡的麦撒燃烧特性消失.TG和DSC分析表明,PbCO3可以降低ADN的初始热分解温度;ADN+5% PbCO3与纯ADN相同失重时对应两者的温度之差随质量损失增加而减小.动力学分析显示,加入5% PbCO3后,ADN热分解表观活化能降低.分析认为,ADN+5% PbCO3+0.2%石蜡和ADN+0.2%石蜡燃烧特性与其表面的ADN熔化层的特征存在关联.

  14. Aortic depressor nerve function examined in diabetic rats by means of two different approaches.

    Science.gov (United States)

    do Carmo, Jussara M; Huber, Domitila A; Castania, Jaci A; Fazan, Valéria P S; Fazan, Rubens; Salgado, Helio C

    2007-03-30

    The present study examined in anesthetized rats, 5 or 120 days after the onset of streptozotocin-induced diabetes, the aortic depressor nerve (ADN) function by means of pressure-nerve activity curve (fitted by sigmoidal regression) and cross-spectral analysis between mean arterial pressure (MAP) and ADN activity. From the sigmoidal regression curve it was calculated the upper and lower ADN activity plateau, range, average gain and MAP halfway between the lower and upper plateau (MAP50). By means of spectral analysis it was calculated the transfer function magnitude (ratio of ADN activity/MAP) as an index of ADN sensitivity (gain) during induced (withdrawal and reinfusion of blood) slow (0.35 Hz) oscillations of MAP simulating Mayer's waves and spontaneous oscillations (approximately 1.5 Hz) caused by respiratory movement. Diabetic rats exhibited, at 5 or 120 days, lower MAP and heart rate. The parameters calculated by means of the sigmoidal regression curve, as well as the ADN activity gain during slow or spontaneous oscillations of MAP, were similar in diabetic and control rats. In conclusion, it was demonstrated that ADN activity was not altered after 5 or 120 days of experimental diabetes, even though the literature documents, at this time frame of diabetes, a conspicuous derangement of the baroreflex.

  15. Aplicaciones de la genética molecular en la practica forense

    OpenAIRE

    Ramos. M., Oscar F.; Yunis T., Emilio; Yunis L., Juan J.

    2011-01-01

    Desde el desarrollo de las huellas digitales a finales del siglo XIX, la tipificación del ADN por VNTR, representa la innovación más poderosa que se haya desarrollado en las ciencias forenses durante el siglo XX. Con el descubrimiento en 1980 del polimorfismo del ADN en el genoma humano y con la subsecuente demostración de que tal hipervariabilidad esta ampliamente distribuida en los humanos, las ciencias forenses han reconocido el enorme potencial que ofrece la tipificación del ADN para la i...

  16. A study of epidemic spreading on activity-driven networks

    Science.gov (United States)

    Zou, Yijiang; Deng, Weibing; Li, Wei; Cai, Xu

    2016-03-01

    The epidemic spreading was explored on activity-driven networks (ADNs), accounting for the study of dynamics both on and of the ADN. By employing the susceptible-infected-susceptible (SIS) model, two aspects were considered: (1) the infection rate of susceptible agent (depending on the number of its infected neighbors) evolves due to the temporal structure of ADN, rather than being a constant number; (2) the susceptible and infected agents generate unequal links while being activated, namely, the susceptible agent gets few contacts with others in order to protect itself. Results show that, in both cases, the larger epidemic threshold and smaller outbreak size were obtained.

  17. Nouveau design de sondes pour biopuces ADN fonctionnelles et caractérisation des capacités de biodégradation des communautés bactériennes de sols pollués par des hydrocarbures

    OpenAIRE

    Terrat, Sébastien

    2010-01-01

    Soil ecosystems are sensitive to damage from pollutions, and there is an increasing need to develop better methods for removing pollutants from soils. The removal of pollutants, such as polycyclic aromatic hydrocarbons, by bioremediation, is a less invasive and expensive process than classical decontamination. However, use and optimization of bioremediation treatments require knowledge on metabolic capacites of microbial communities involved in the biodegradation of such pollutants. To assess...

  18. Correlation of the radioprotective effect of the methyl gallate on the ruptures induction in DNA and it effect in the capture of free radicals; Correlacion del efecto radioprotector del metil galato sobre la induccion de rupturas en el ADN y su efecto en la captura de radicales libres

    Energy Technology Data Exchange (ETDEWEB)

    Morales R, P.; Cabral P, A.; Cruz V, V.L.; Gonzalez B, F.; Zarco M, A. [ININ, 52750 La Marquesa, Estado de Mexico (Mexico)

    2007-07-01

    It is shown in alive, the capacity of the methyl gallate to reduce the induced ruptures in the DNA for {gamma} radiation. As well as to capture free radicals in a system in vitro. This suggests that the methyl gallate can be a radioprotector that acts capturing free radicals. (Author)

  19. Evidence of a genetic instability induced by the incorporation of a DNA precursor marked with tritium; Mise en evidence d'une instabilite genetique induite par l'incorporation d'un precurseur de L'ADN marque au tritium

    Energy Technology Data Exchange (ETDEWEB)

    Saintigny, Y.; Laurent, D.; Lahayel, J.B. [CEA Fontenay-aux-Roses, IRCM-LRTS, U967 - CEA/INSERM/Universites Paris 7 and Paris-11, 92 (France); Roche, St.; Meynard, D.; Lopez, B.S. [CEA Fontenay-aux-Roses, LMR - UMR 217 - CEA/CNRS, Institut de Radiobiologie Cellulaire et moleculaire, Direction des Sciences du Vivant, 92 (France)

    2009-07-01

    The authors report a molecular geno-toxicology investigation which allowed molecular events induced par intracellular incorporation of tritium to be studied, and the genetic instability resulting from a chronic exposure even at low dose to be analysed. For this purpose, they developed cell models (hamster tumorous cells and human fibroblasts) in which they know how to incorporate given quantities of marked nucleotides in the DNA. They show that the incorporation of tritium, even with doses which are said to be non toxic, causes a prolonged exposure of the cell to a genotoxic stress, and maybe a genetic instability due to a too great number of recombination events

  20. Evaluación de los niveles del aducto 8-OHdG y fragmentación del ADN en linfocitos periféricos humanos expuestos in vitro a hidrocarburos aromáticos policíclicos con y sin activación metabólica animal /

    OpenAIRE

    Rodríguez Romero, María Isabel

    2011-01-01

     tesis que para obtener el grado de Doctor en Ciencias Biológicas, presenta María Isabel Rodríguez Romero ; asesor María Elena Calderón Segura, Sandra Luz Gómez Arroyo, Luis Felipe Jiménez García. 106 páginas : diagramas. Doctorado en Ciencias Biológicas UNAM, Instituto de Geología, 2011

  1. Cellular signal adaptation with damage control at low doses versus the predominance of DNA damage at high doses; Perturbation de la signalisation cellulaire apres irradiation a faible dose contre la predominance des lesions de l'ADN a dose elevee

    Energy Technology Data Exchange (ETDEWEB)

    Feinendegen, L.E. [Clinical Center, National Institutes of Health, Bethesda (United States). Dept. of Nuclear Medicine; Bond, V.P. [Washington State Univ., Richland, WA (United States). Research Faculty; Sondhaus, Ch.A. [Arizona Univ., Tucson, AZ (United States). Dept. of Radiology and Radiation Control Office; Altman, K.I. [Rochester Univ., NY (United States). Dept. of Biochemistry and Biophysics

    1999-03-01

    Ionizing radiation is known to potentially interfere with cellular functions at all levels of cell organization and induces DNA lesions apparently with an incidence linearly related to D, also at low doses. On the other hand, low doses have also been observed to initiate a slowly appearing temporary protection against causation and accumulation of DNA lesions, involving the radical detoxification system, DNA repair and removal of DNA damage. This protection apparently does not operate at high doses; it has been described to be nonlinear, increasing initially with D, beginning to decrease when D exceeds approximately 0.1-0.2 Gy and eventually disappearing at higher D. The various adaptive responses have been shown to last individually from hours to weeks in different cell types and resemble responses to oxidative stress. Damage to DNA is continuously and endogenously produced mainly by reactive oxygen species (ROS) generated in a normal oxidative metabolism. This endogenous DNA damage quantitatively exceeds DNA damage from low-dose irradiation, by several orders of magnitude. Thus, the protective responses following acute low-dose irradiation may be presumed to mainly counteract the endogenous DNA damage. Accordingly, the model described here uses two dose-effect functions, a linear one for causing and a nonlinear one for protecting against DNA damage from whatever cause in the irradiated cells and tissues. The resulting net dose-risk function strongly suggests that the incidence of cancer versus dose in the irradiated tissues is much less likely to be linear than to exhibit a threshold. The observed cancer incidence may even fall below the spontaneous incidence, when D to cells is below approximately 0.2 Gy. However incomplete, these data support a reexamination of the LNT hypothesis. (authors)

  2. Efecto del tiempo y la temperatura en la viabilidad del ADN en la perfilación genética de muestras de sangre/Effects of time and temperature in the viability of DNA in genetic profiling of blood samples

    Directory of Open Access Journals (Sweden)

    Christian Ramón Hernández Sánchez

    2015-01-01

    Full Text Available In this study has been investigated the feasibility of obtaining DNA genetic profiling of biological samples from male human blood subject temperature and humidity factors. The methodology consisted of a sample preparation, DNA extraction, PCR amplification of genetic marker Amelogenin and finally DNA sequencing, we determined the incidence of effective amplification to obtain a complete profile, partial or no sample problem. Furthermore human blood samples over eight days of exposure showed a lower amplification. This research seeks to level the playing field having a scene, the type of samples found, so that the information gathered in this research is very useful trying to help establish viable which samples are to be analyzed in the laboratory.

  3. Influencia de los lípidos de la dieta en la iniciación del cáncer de mama experimental: Ontogenia de las enzimas de detoxificación de xenobióticos. Metabolism ode carcinógeno y daño en el ADN

    OpenAIRE

    Manzanares Serrano, Miguel Angel

    2014-01-01

    El cáncer de mama es el más frecuente en las mujeres de todo el mundo. Además de los factores genéticos, epigenéticos y hormonales, existen evidencias epidemiológicas y experimentales de que los factores nutricionales y ambientales tienen un papel en la etiología y el desarrollo de esta enfermedad. Los lípidos de la dieta se han relacionado directamente con el cáncer, fundamentalmente, el de mama. El grupo de investigación ha contribuido al mejor conocimiento de los efectos y de los mecanismo...

  4. Concentration measurement of carbon monoxide in the combustion chamber of ADN-based thruster with QCL%基于量子级联激光器的ADN基液体发动机稳态燃烧CO特征浓度的实验测量

    Institute of Scientific and Technical Information of China (English)

    张伟; 沈岩; 姚兆普; 余西龙; 曾徽; 李飞

    2015-01-01

    二硝酰胺铵(AND)作为一种绿色无毒推进剂已在固体推进剂以及液体推进剂领域得到一定程度的应用,是未来化学推进技术的一个新方向.目前我国正处于ADN基液体推进剂研究的起始阶段,对于其燃烧反应过程的研究是当前的一个研究重点.由于ADN基液体推进剂催化分解及燃烧反应过程复杂,相关的实验结果国内外公开文献发表较少,因此有必要开展ADN基液体发动机催化分解及燃烧反应的实验研究.本实验采用基于量子级联激光器的吸收光谱诊断技术,对ADN基液体发动机燃烧室内燃烧过程进行探究.CO是ADN基液体推进剂催化分解及燃烧反应的特征产物之一,通过测量ADN基液体发动机典型工况下CO的特征浓度,可以定量获得ADN基发动机中描述催化分解及燃烧反应进程的重要信息,研究结果可以为我国自主开发高效稳定的液体ADN基无毒空间发动机提供理论基础.

  5. DATOS POBLACIONALES DE LOS MICROSATÉLITES DE ADN HUMANO D2S1338, D19S433, PENTA D, PENTA E Y SE-33 DE LA REGIÓN CENTRAL COLOMBIANA Population Data Of Human DNA Microsatellites D2S1338, D19S433, Penta D, Penta E And SE-33 From Central Region In Colombia

    Directory of Open Access Journals (Sweden)

    MAURICIO REY

    Full Text Available Los microsatélites o STR por su sigla en inglés (Short Tandem Repeat son repeticiones de secuencias cortas de nucleótidos a través del DNA, de gran importancia debido a su carácter polimórfico que permite su utilización en la identificación genética de individuos o poblacionales. En este trabajo se investigaron los perfiles genéticos de una muestra de poblaciones humanas del altiplano cundiboyacense en Colombia; se usaron los marcadores STR's D2S1338, D19S433, PENTA D, PENTA E y SE-33. Éstos cinco STR's fueron amplificados mediante reacción en cadena de la polimerasa (PCR, corridos con electroforesis capilar, y tipificados con los programas Genscan y Genotyper. Como resultado se reportan las frecuencias alélicas de los cinco microsatélites (no reportados anteriormente para esta región. Se encontró que los loci D19S433 y SE-33 no están en equilibrio de Hardy-Weinberg; y que el Penta E tiene el mayor poder de discriminación. La distribución de las frecuencias alélicas para los cinco marcadores, en las dos poblaciones analizadas mostró que no existen diferencias estadísticas significativas entre ellas.Microsatellites (STR's are short tandem repeat sequences of nucleotides through DNA, very important for their polymorphic character that allow their utilization in population genetic identification. The STR's markers D2S1338, D19S433, PENTA D, PENTA E y SE33 were used to do the genetic profiles of human populations from the Cundiboyacense region in Colombia. The five STR's were amplified by PCR (polymerase chain reaction, indentified with capillary electrophoresis, and typified with Genscan and Genotyper software. Allelic frequencies of the five microsatellites are reported (not previously reported for this region. It was found that loci D19S433 y SE-33 are not in Hardy-Weinberg equilibrium; and Penta E provides the greatest power of discrimination. The allelic frequencies distribution to these five markers, in the two populations studied show that it does not exist statistically significant differences between them.

  6. Signalization and repair of the DNA double-strand breaks of in the cerebral tumors: modulation of the radiation response with the chemotherapy treatments; Signalization et reparation des cassures double-brin de l'ADN dans les gliomes: modulation de la reponse aux traitements chimio-radiotherapeutiques

    Energy Technology Data Exchange (ETDEWEB)

    Marcinkova-Bencokova, Z

    2007-07-15

    There are about 6000 new cases of nervous system tumours each year in France. However, the current radio chemotherapeutic approaches against brain tumours remain still insufficient to produce a satisfactory therapeutic index. In parallel, the knowledge of the early radiobiological events has considerably progressed in the last few years. This thesis aims to provide new insights in the molecular and cellular response of brain tumours to radio chemotherapy. This thesis was divided into four stages. Stage 1: a novel DNA double-strand breaks repair pathway depending on the MRE11 protein but independent of the phosphorylation of H2AX emerged from the study of artefacts of the immunofluorescence technique and a systematic analysis of the radiosensitivity of human cells. Stage 2: the radiobiological features of 3 rodent models of glioma among the most used in preclinical trials and of 7 human glioma cell lines were investigated. Functional impairments of the BRCA1 protein in response to radiation and/or cisplatin were observed in the majority of the models tested, raising the question of the role of this protein in the anti-glioma treatments and in glioma genesis. Stage 3: in order to extend our approach to genetic syndromes associated with cerebral tumours predisposition, the radiobiological characteristics of the fibroblasts resulting from patients suffering from neurofibromatosis type 1 (NF1), a pathology associated with a strong incidence of peripheral nervous system tumours, were investigated. NF1 appeared to be a syndrome with moderated radiosensitivity, associated with a weak deficiency of DNA end-joining repair but with a strong activity of MRE11. These results enabled us to propose a preliminary model involving both proteins BRCA1 and NF1. Stage 4: considering the role of BRCA1 in the inhibition of some tyrosine kinase activity and in the response to cisplatin, we tested the radiobiological effects of treatments combining radiation, cisplatin and tyrosine kinase inhibitors to human and rodent gliomas. Our results encourage the personalization of the treatments against brain tumours by taking into account the functionality of some proteins, like BRCA1, that act upstream of the currently studied stress signalling pathways. (author)

  7. Neurosifilis en pacientes con infeccion por VIH. Evaluacion de la repuesta al tratamiento mediante la deteccion del ADN que codifica la proteina de membrana de 47 kDa del T pallidum en LCR por la reaccion en cadena de la polimerasa

    OpenAIRE

    Bordón Ferreyra, José M.

    2000-01-01

    Numerosos trabajos describieron un incremento de la prevalencia de la sifilis en laultima decada. La reaparicion de la sifilis fue ademas descrita asociada a presentacionesatipicas en pacientes con infeccion por VIH asi como de importantes controversias en eldiagnostico serologico y repuesta al tratamiento estandarizado. Esta informacion deriva decasos clinicos y pequenas series de pacientes con infeccion por VIH-1 en quienes seencontro importantes limitaciones en la interpretacion de la ser...

  8. Neurosífilis en pacientes con infección por VIH. Evaluación de la respuesta al tratamiento mediante la detección del ADN de la proteína de membrana de 47 kDa del T pallidum en LCR por la reacción en cadena de la polimerasa

    OpenAIRE

    Bordón Ferreyra, José M.

    2002-01-01

    Consultable des del TDX Títol obtingut de la portada digitalitzada Numerosos trabajos describieron un incremento de la prevalencia de la sifilis en laultima decada. La reaparicion de la sifilis fue ademas descrita asociada a presentacionesatipicas en pacientes con infeccion por VIH asi como de importantes controversias en eldiagnostico serologico y repuesta al tratamiento estandarizado. Esta informacion deriva decasos clinicos y pequenas series de pacientes con infeccion por VIH-1 en qu...

  9. Investigation of the effect of ionizing radiation on gene expression variation by the 'DNA chips': feasibility of a biological dosimeter; Etude de l'effet des radiations ionisantes sur la variation d'expression genique par la methode des 'puces a ADN': faisabilite d'un dosimetre biologique

    Energy Technology Data Exchange (ETDEWEB)

    Gruel, G.

    2005-05-25

    After having described the different biological effects of ionizing radiation and the different approaches to biological dosimetry, and introduced 'DNA chips' or DNA micro-arrays, the author reports the characterization of gene expression variations in the response of cells to a gamma irradiation. Both main aspects of the use DNA chips are investigated: fundamental research and diagnosis. This research thesis thus proposes an analysis of the effect of ionizing radiation using DNA chips, notably by comparing gene expression modifications measured in mouse irradiated lung, heart and kidney. It reports a feasibility study of bio-dosimeter based on expression profiles

  10. A new cryptic species of Leberis Smirnov, 1989 (Crustacea, Cladocera, Chydoridae from the Mexican semi-desert region, highlighted by DNA barcoding Una nueva especie críptica de Leberis Smirnov, 1989 (Crustacea, Cladocera, Chydoridae procedente de una región semidesértica mexicana, evidenciada por los códigos de barras del ADN

    Directory of Open Access Journals (Sweden)

    Manuel Elías-Gutiérrez

    2008-03-01

    Full Text Available DNA barcoding, based in the sequence of a gene from the mitochondria, the Citochrome C oxidase (CO1, has been proved to be an excellent tool to identify many animal groups from invertebrates to vertebrates. After barcode several Cladocera, we gathered evidence about the existence of two Leberis species in the north of Mexico. Minimal CO1 divergence between both taxa was 14.3%, due to the GC% in the third codon position. A detailed morphological analyses uncovered one of the species as L. davidi, dwelling from South America to Mexico and the other as Leberis chihuahuensis new species, apparently restricted to semi-desert temporary pools. The parthenogenetic female of the latter is characterized by a small size, blunt rostrum, long and curved spine in the first exopodal segment of the second antenna, absence of accessory seta and sensilla in the first thoracic limb, and a brush-shaped seta in the fourth limb, among other characters. Males have a long and narrow postabdomen and a series of similar sized spinules in the posterior margin of the valves. By the first time a cladoceran is described from both views, the morphology and CO1 sequence.Recientemente una técnica molecular denominada códigos de barras, basada en secuenciar una fracción del gen mitocondrial que codifica para la citocromo oxidasa C (conocido también como CO1 ha demostrado ser una excelente herramienta para identificar especies animales, desde invertebrados hasta vertebrados. Al aplicar esta técnica a cladóceros de agua dulce de diversos lugares, se obtuvo evidencia de la presencia de dos especies del género Leberis en el norte de México. La mínima divergencia en el CO1 entre ambos taxa fue de 14.3%, debida principalmente al contenido de Guanina-Citosina en la posición del tercer codon. El análisis detallado de las estructuras anatómicas de ambas especies permitió identificar a una de ellas como L. davidi, distribuida desde Sudamérica hasta México y la otra corresponde a L. chihuahuensis sp. nov., aparentemente restringida a charcos temporales de regiones semidesérticas. En esta última, la hembra partenogenética se caracteriza por su talla pequeña, el rostro redondeado, la forma y longitud de la espina del primer segmento exopodal de la segunda antena, la ausencia de setas accesorias y sensilas en el lóbulo distal externo del primer apéndice torácico y la presencia de una seta con forma de brocha en el cuarto apéndice torácico, entre otras características. El macho posee un postabdomen largo y angosto, y una serie de espinas de igual tamaño en el margen posterior de las valvas. En este trabajo se conjunta por primera vez la descripción morfológica detallada con la secuencia del CO1 para describir una nueva especie de este grupo.

  11. Theoretical study of some aspects of the nucleo-bases reactivity: definition of new theoretical tools for the study of chemical reactivity; Etude theorique de quelques aspects de la reactivite des bases de l'ADN: definition de nouveaux outils theoriques d'etude de la reactivite chimique

    Energy Technology Data Exchange (ETDEWEB)

    Labet, V.

    2009-09-15

    In this work, three kinds of nucleo-base damages were studied from a theoretical point of view with quantum chemistry methods based on the density-functional theory: the spontaneous deamination of cytosine and its derivatives, the formation of tandem lesion induced by hydroxyl radicals in anaerobic medium and the formation of pyrimidic dimers under exposition to an UV radiation. The complementary use of quantitative static methods allowing the exploration of the potential energy surface of a chemical reaction, and of 'conceptual DFT' principles, leads to information concerning the mechanisms involved and to the rationalization of the differences in the nucleo-bases reactivity towards the formation of a same kind of damage. At the same time, a reflexion was undertaken on the asynchronous concerted mechanism concept, in terms of physical meaning of the transition state, respect of the Maximum Hardness Principle, and determination of the number of primitive processes involved. Finally, a new local reactivity index was developed, relevant to understand the reactivity of a molecular system in an excited state. (author)

  12. Determination of the adaptive response induced In vivo by gamma radiation and its relation with the sensibility to the damage induction in the DNA and with the repairing capacity; Determinacion de la respuesta adaptativa inducida In vivo por radiacion gamma y su relacion con la sensibilidad a la induccion de dano en el ADN y con la capacidad de reparacion

    Energy Technology Data Exchange (ETDEWEB)

    Mendiola C, M.T

    2002-07-01

    The kinetics of damage induction and repair at different doses as well as the adaptive response induced by gamma ray exposure were determined in murine leukocytes in vivo. The damage-repair kinetics were established after the exposure to 0.5, 1.0 or 2.0 Gy in a {sup 137}Cs source. Peripheral blood samples were obtained from the tails of mice, the percentage of damaged cells and the DNA migration in each one were analyzed by the single cell gel electrophoresis (SCG) technique or comet assay. Results indicated that there was an induction of approximately 75% comets with the doses of 1.0 and 2.0 Gy, which was considerably reduced to 22% and 42% respectively during the first 15 minutes. This evidences the presence of a rapid repair process and suggests that leucocytes are genetically well prepared to repair this kind of damage. After 15 minutes, a second increase in the percentage of damaged cells that was proportional to dose occurred, which seems to represent the breaks produced during the repair of other kind of lesions. After that a second reduction was observed, reaching values near to the basal ones, except with the dose of 2.0 Gy. The kinetics obtained with the dose of 0.5 Gy was similar to that established with 1.0 Gy, but in this case the initial damage was 50 % lower. Besides, the adaptive response was observed after the exposure of the mice to an adaptive dose of 0.01 Gy and to a challenge dose of 1.0 Gy 60 minutes later. The pretreatment reduced the percentage of damaged cells caused by the challenge dose to one third approximately, and also diminished this parameter produced during the late repair process. This indicates that the early adaptive response is caused, instead of by an increment in repair, by the induction of a process that protects DNA from damage induction by radiation, i.e synthesis of substances that increase the scavenging of free radicals. (Author)

  13. Knowledge adn application of the International Human Right and the Medical Mission in the emergency services in the east region of Antioquia Conocimiento y aplicación del Derecho Internacional Humanitario y la Misión Médica en los servicios de urgencias del oriente antioqueño

    Directory of Open Access Journals (Sweden)

    Carlos Andrés Villa B.

    2003-03-01

    Full Text Available In october ctober 2002 a descriptive study was made to find out the grade of knowledge in the Emergency Services of the East region of Antioquia of the International Human Right, (IHR and the Medical Mission (MM. This region has been suffering since many years the consequences of the war that show what has been happening in the entire country. A questionnaire was filled by the directors of the hospitals in this region about both items. They agree on the importance of the items but said they have little knowledge about them and they have received few instructions regarding those items. In their hospitals there are neither guides nor education programs for the personnel in this regard. To be posted in a hospital, a person needs not show proficiency in IHR or MM. En octubre de 2002 se llevó a cabo un estudio descriptivo de corte con el propósito de investigar sobre el conocimiento y la aplicación del Derecho Internacional Humanitario (DIH y la Misión Médica (MM, en los servicios de urgencias localizados en municipios del oriente del departamento de Antioquia, región que sufre desde hace varios años las consecuencias del conflicto armado colombiano y que refleja la problemática general del país. El estudio se hizo encuestando a los directores de esos servicios de urgencias para averiguar qué formación habían tenido sobre DIH y MM y qué pautas había en las instituciones que representaban para la aplicación de estas normas. Los directores encuestados manifestaron tener muy poca información acerca del DIH y la MM. Durante su formación académica de pregrado y posgrado no recibieron educación formal al respecto. Estuvieron de acuerdo en la importancia del tema, la necesidad de capacitarse y la posibilidad de aplicarlo. Por otra parte, en las instituciones de salud que cuentan con servicios de urgencias en el oriente antioqueño se carece de protocolos o manuales sobre el DIH y la MM así como de actividades de formación continua que permitan al personal sanitario actuar a la luz de estas normas; además, el conocimiento previo de estos aspectos no es requisito para laborar en las instituciones de salud que cuentan con servicios de urgencias en la región.

  14. Bulky PAH-DNA induced by exposure of a co-culture model of human alveolar macrophages and embryonic epithelial cells to atmospheric particulate pollution; Adduits encombrants a l'ADN dans des cocultures de cellules pulmonaires humaines exposees a une pollution atmospherique particulaire

    Energy Technology Data Exchange (ETDEWEB)

    Abbas, Imane; Garcon, Guillaume; Billet, Sylvain; Shirali, Pirouz [Universite Lille Nord de France - Lille (France); Unite de Chimie Environnementale et Interactions sur le Vivant, MREI, Universite du Littoral Cote d' Opale, Dunkerque (France); Andre, Veronique; Le Goff, Jeremie; Sichel, Francois [GRECAN, Universite de Caen Basse-Normandie et centre Francois Baclesse, Caen (France); Roy Saint-Georges, Francoise; Mulliez, Philippe [Service de Pneumologie, Hopital Saint-Philibert, GHICL, Lille (France)

    2012-01-15

    Because of their deep penetration in human lungs, fine airborne particulate matter were described as mainly responsible for the deleterious effects of exposure to air pollution on health. Organic constituents are adsorbed on particles surface and, after inhalation, some (polycyclic aromatic hydrocarbons, PAHs) can be activated into reactive metabolites and can bind to DNA. The formation of bulky DNA adducts has been researched after exposure of mono-and co-cultures of alveolar macrophages (AM) and human embryonic human lung epithelial (L132), to fine air pollution particulate matter Air samples have been collected with cascade impactor and characterized: size distribution (92.15% < 2.5{mu}.m), specific surface area (1 m{sup 2}/g), inorganic (Fe, AI, Ca, Na, K, Mg, Pb, etc.) and organic compounds (PAHs, etc.). {sup 32}P post-labeling method was applied to detect bulky DNA adducts in AM and L132, in mono-and co-cultures, 72 h after their exposure to atmospheric particles at their Lethals and Effects concentrations or (LC or CE) to 50% (i.e. MA: EC{sub 50} = 74.63 {mu}g/mL and L132: LC-5-0 = 75.36 {mu}g/mL). Exposure to desorbed particles (MA: C1= 61.11 {mu}g/mL and L132 : C2 = 61.71 {mu}g/mL) and B[a]P (1 {mu}M) were included. Bulky PAH-DNA adducts were detected in AM in mono-culture after exposure to total particles (Pt), to B[a]P and desorbed particles (Pd). Whatever the exposure, no DNA adduct was detected in L132 in mono-culture. These results are coherent with the enzymatic activities of cytochrome P450 l Al in AM and L132. Exposure of co-culture to Pt, or Pd induced bulky adducts to DNA in AM but not in L132. Exposure to B[a]P alone has altered the DNA of AM and L132, in co-culture. Exposure to Pt is closer to the environmental conditions, but conferred an exposure to amounts of genotoxic agents compared to studies using organic extracts. The formation of bulky DNA adducts was nevertheless observed in AM exposed to Pt, in mono- or co-culture, indicating that they were competent in terms of metabolic activation of PAHs. The DNA damage in the L132 in co-culture following exposure to B[a]P have suggested that some PAH metabolites generated by the MA could be have a genotoxic action on L132. (authors)

  15. Investigation of double strand breaks induced by alpha particle irradiation using C.N.B.G. microbeam in human keratinocytes; Mise en evidence de cassures double brin de l'ADN induites par irradiation de keratinocytes humains en microfaisceau alpha

    Energy Technology Data Exchange (ETDEWEB)

    Pouthier, Th

    2006-12-15

    To understand the mechanisms of interaction of ionizing radiation with living tissues exposed to low and protracted doses remains a major issue for risk evaluation. The response cannot be found in epidemiological studies because the only available data concern accidental exposures to high doses of radiation. The natural exposure represents the main source of exposure in the daily life, just before the medical sources (radiology, radiotherapy). In addition, this kind of exposure is very difficult to reproduce in vitro by irradiating cell lines. The method per preference is based on random irradiation of cell populations. The mean number of particles having traversed cells is then calculated on the basis of Poisson statistics. In addition to inevitable multiple impacts, the numerous potential intracellular targets (nuclei, cytoplasm), the indirect effects induced by the impact of particles on neighbouring cells or simply the extracellular targets, constitute phenomena that make more complex the interpretation of experimental data. A charged particle microbeam was developed at C.E.N.B.G. to perform the targeted irradiation of individual cells with a targeting precision of a few microns. It is possible to deliver a counted number of alpha particles down to the ultimate dose of one alpha per cell, to target predetermined cells and then to observe the response of the neighbouring cells. This facility has been validated during this work on human keratinocyte cells expressing a recombinant nuclear fluorescent protein (histone H2B-GFP). The combination of ion micro-beams with confocal microscopy and numeric quantitative analysis allowed the measurement of DNA double strand breaks via the phosphorylation of the histone H2A.X in individual cells. The mechanisms of DNA reparation and apoptosis induction were also in the scope of those studies. The experimental results obtained during this thesis validate the methodology we have developed by demonstrating the targeting reproducibility and the dose control. A dose-effect relationship has been also investigated as a function of time. (author)

  16. Fibroblast growth factor 2 and DNA repair involvement in the keratinocyte stem cells response to ionizing radiation; Implication du FGF2 (fibroblast growth factor 2) et la reparation de l'ADN dans la reponse des keratinocytes souches aux irradiations

    Energy Technology Data Exchange (ETDEWEB)

    Harfouche, L' Emira Ghida

    2010-02-15

    Keratinocyte stem cells (KSCs) from the human inter follicular epidermis are regarded as the major target to radiation during radiotherapy. We found herein that KSCs are more resistant to ionizing radiation than their direct progeny, and presented more rapid DNA damage repair kinetics than the progenitors. Furthermore, we provided evidence describing the effect of fibroblast growth factor 2 (FGF2) signaling on the ability of KSCs and progenitors to repair damaged DNA. Despite our knowledge of the fact, that FGF is an anti-apoptotic factor in multiple cell types, the direct link between DNA repair and FGF2 signaling has rarely been shown. Existence of such link is an important issue with implications not only to stem cell field but also to cancer therapy. (author)

  17. Biogenética e género na construção da intencionalidade da paternidade: o teste de DNA nas investigações judiciais de paternidade Bio-genetics and gender in the construction of the paternity intentionality: ADN testing in the judicial investigations of paternity

    Directory of Open Access Journals (Sweden)

    Helena Machado

    2011-12-01

    Full Text Available Este artigo aborda as modalidades de intencionalidade da paternidade construídas por mulheres e homens que realizaram testes de DNA ordenados por tribunais, em Portugal, para apuramento da paternidade biológica de crianças sem 'pai oficial'. Partindo de uma perspectiva feminista, analisa-se o impacto das ideologias de género na mediação da intenção de desempenhar o papel de pai, na negociação de relações de parentesco e nos processos sociais e morais de classificação e hierarquização dos indivíduos. Conclui-se que a incorporação do conhecimento do resultado do teste de DNA nas práticas quotidianas de homens e mulheres constitui uma co-produção complexa entre as relações sociais de género, a cultura, a tecnologia e o sistema jurídico.This article focuses on the modes of intentionality of paternity constructed by women and men who went through the process of DNA paternity testing by a court order, in Portugal, in the context of paternity investigations of children without an "official father". Drawing from a feminist perspective, we analyse the impact of gender ideologies on the mediation of the intention to perform the role of the father, the negotiation of kinship relations and the social and moral processes of individuals' classification and hierarchization. We conclude that the incorporation of the knowledge ensuing from the result of the DNA test in the daily practices of men and women constitutes a complex co-construction between social relations of gender, culture, technology and law.

  18. Intra-specific brood parasitism revealed by DNA micro-satellite analyses in a sub-oscine bird, the vermilion flycatcher Parasitismo intraespecífico revelado mediante análisis de microsatélites de ADN en un ave suboscina, el cardenalito o saca tu real

    Directory of Open Access Journals (Sweden)

    ALEJANDRO A RÍOS-CHELÉN

    2008-03-01

    Full Text Available Extra-pair reproduction is known to occur in many avian species. However, among passerines, the majority of studies on extra-pair reproduction have been carried out in oscine birds from temperate regions. Conversely, sub-oscines species, and particularly, species that inhabit tropical regions, have been studied to a much lesser extent. Given that a majority of avian species live in the tropics, it is important to study more tropical and sub-oscine species to have a more accurate picture of the rates of extra-pair reproduction among passerines, and a better understanding of the adaptive function of extra-pair reproduction in birds. Tropical species differ from temperate species in several ecological and life history traits, that may influence the occurrence of different modes of extra-pair reproduction and their prevalence. In this study we asked whether extra-pair reproduction occur in a sexually dimorphic and socially monogamous sub-oscine, the vermilion flycatcher (Pyrocephalus rubinus. We report cases of extra-pair paternity, extra-pair maternity and intra-specific brood parasitism, and discuss our results in the view of other studies with passerinesSe sabe que la reproducción extrapareja ocurre en muchas especies de aves. Sin embargo, entre paserinos, la mayoría de los estudios se han llevado a cabo en aves oscinas de regiones templadas. Por el contrario, las especies suboscinas, y en particular las especies que habitan regiones tropicales, se han estudiado mucho menos. Por lo tanto, es importante estudiar más especies tropicales y suboscinas para tener una visión más acertada de las tasas de reproducción extrapareja en paserinos y un mejor entendimiento de la función adaptativa de la reproducción extrapareja en aves. Las especies tropicales difieren de las especies que habitan regiones templadas en diversos rasgos ecológicos y de historia de vida, que podrían influir en las tasas de ocurrencia de diferentes modos de reproducción extrapareja. En este estudio nos preguntamos si ocurre o no la reproducción extrapareja en el cardenalito o saca tu real (Pyrocephalus rubinus, un suboscino sexualmente dimórfico y socialmente monógamo. Reportamos casos de paternidad extrapareja, maternidad extrapareja y parasitismo intraespecífico, y discutimos nuestros resultados bajo la luz de otros estudios en paserinos

  19. Dose-response relationship of induction kinetics of In vivo DNA damage and repair in mouse leukocytes exposed to gamma radiation; Relacion dosis-respuesta de la cinetica de induccion de dano y reparacion del ADN In vivo en leucocitos de raton expuestos a radiacion gamma

    Energy Technology Data Exchange (ETDEWEB)

    Mendiola C, M.T.; Morales, R.P. [Instituto Nacional de Investigaciones Nucleares, Gerencia de Investigacion Basica, Departamento de Biologia, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    2000-07-01

    The Unicellular electrophoresis in gel technique is a useful tool in the determination of simple ruptures and labile sites to the alkali in DNA of eucariontes cells. The determination of the induction kinetics of damage and repair of DNA can give more information. The objective of this work was to determine whether the analysis of the area under the damage/repair induction kinetics curve in comets percent or the comets frequency in the two peaks of maximum induction is adequate for determining the dose-response relationship. The mice were exposed at the doses of 0.5, 1.0, 2.0 Gy. (Author)

  20. Correlation between urinary concentrations of malondialdehyde and DNA damage in people exposed to mercury = Correlación entre la concentración urinaria de malondialdehído y daño en el ADN de personas expuestas a mercurio

    Directory of Open Access Journals (Sweden)

    Castaño Arias, Paula Andrea

    2014-03-01

    Full Text Available Objective: To determine whether the extent of DNA damage is correlated with the concentration of malondialdehyde (MDA in urine of individuals occupationally exposed and unexposed to mercury Methods: We evaluated 64 medical records (32 reference group and 32 exposed group, they were matched byage, smoke and alcohol consumption. For analysis, comet assay data (olive tail moment, % DNA in the tail and tail length, levels of urinary MDA and urinary mercury were taken. We compared the concentrations of MDA and the changes in the comet assay between the groups and the correlation between these variables. Results: MDA concentrations were higher in exposed than in referents (median 1.28 vs 0.51μmol/L, respectively and further damage to the comet assay (mean of tail % DNA: 27.37 vs. 0.31, median of Olivetail moment 8.29 vs olive 0.056; median of tail length 35 vs. 3.0, respectively.However there was a poor correlation between urinary MDA and genetic damage(r<0.11. Conclusion: No evidence was obtainedwith higher concentrations of MDA in urine provide additional genetic damage, but there was more DNA damage and higher concentrations of MDA in individuals occupationally exposed to mercury.

  1. Metilación de ADN y expresión de antígenos de histocompatibilidad de clase I en células tumorales Javier Martín Ibáñez; [directores] Federico Garrido Torres-Puchol, E. Pareja Tello, Antonia Aránega Jiménez

    OpenAIRE

    Martín Ibáñez, Javier

    2011-01-01

    Sobre dos clonos-tumorales murinos (a7 y b9) derivados del mismo tumor sólido inducidos en ratones de la cepa balb/c mediante metilcolantreno se investiga la expresión e inducción de antígenos de histocompatibilidad clase i. Para ello se tratan células del clon b-9 con inductores de ag clase i (gamma-interferon tpa y ácido retinoico). Con tpa y ac. retinoico no se consigue aumento de la expresión sin embargo con interferon se obtiene un claro aumento de la expresión de antígenos clase i en ...

  2. Familiarization and Detection of Green Monopropellants Project

    Data.gov (United States)

    National Aeronautics and Space Administration — ADN was developed by the Swedish Space Corporation (SSC), while HAN was developed by the Air Force Research Laboratory to be a propellant. Alliant Techsystems Inc...

  3. A preliminary contaminant and toxicological survey of Illinois River sediments

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Sediments from 6 sites on the Illinois River adn its tributaries were analyzed for organic and inorganic contaminants. Relative toxicity of sediments was determined...

  4. Distribution of dominant calanoid copepod species in the Greenland Sea during late fall; 06 November 1988 to 12 December 1988 (NODC Accession 0000917)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Between 6 November and 12 December 1988, vertical distributions of Calanus finmarchicus, C. hyperboreus, C. glacialis adn Metridia long were studied at three...

  5. Do Associate Degree Registered Nurses Fare Differently in the Nurse Labor Market Compared to Baccalaureate-Prepared RNs?

    Science.gov (United States)

    Auerbach, David I; Buerhaus, Peter I; Staiger, Douglas O

    2015-01-01

    Roughly 40% of the nearly 3 million registered nurses (RNs) in the United States have an associate's degree (ADN) as their highest level of nursing education. Yet even before the recent Institute of Medicine report on The Future of Nursing, employers of RNs have increasingly preferred baccalaureate-prepared RNs (BSNs), at least anecdotally. Data from the American Community Survey (2003-2013) were analyzed with respect to employment setting, earnings, and employment outcomes of ADN and BSN-prepared RNs. The data reveal a divergence in employment setting: the percentage of ADN-prepared RNs employed in hospitals dropped from 65% to 60% while the percentage of BSN-prepared RNs employed in hospitals grew from 67% to 72% over this period. Many ADNs who would have otherwise been employed in hospitals seem to have shifted to long-term care settings.

  6. Hydrolysis of Adiponitrile in Near-critical Water

    Institute of Scientific and Technical Information of China (English)

    DUAN Pei-Gao; NIU Yan-Lei; WANG Yuan-Yuan; DAI Li-Yi

    2008-01-01

    Hydrolysis of adiponitrile (ADN) in near-critical water was successfully conducted in a batch reactor.Influences of m(AND)/m(water) ratio,temperature,time,m(AND)/m(additive) ratio,kind of additive and pressure on the yield of each product were investigated.Five compounds resulting from the hydrolysis of ADN,including 5-cyanovaleramide,adipamide,adipamic acid,adipic acid and trace of 5-cyanovaleric acid,were detected by high performance liquid chromatography.The results showed that change of ADN concentration and temperature had significant influences on the yields of adipamide,adipamic acid and adipic acid;time was the significant factor for the yield of 5-cyanovaleric acid;and the yield of 5-cyanovaleramide was more dependent on the ADN concentration.

  7. Preparation of Energetic Composite——The Coating of AND%含能复合材料的制备——ADN的包覆

    Institute of Scientific and Technical Information of China (English)

    杨帆

    2008-01-01

    ADN是一种很有前途的新型氧化剂,可以应用在火炸药及导弹推进剂中.但其吸湿性较强,这是影响其应用的主要问题.利用制备复合粒子的方法对ADN进行包覆,以降低ADN的吸湿性.实验过程中主要制备了ADN/AP复合粒子和ADN/碳纳米管两种复合粒子.结果表明,ADN/AP复合粒子的吸湿性与原ADN相比有明显降低,尤其是乙醇/二氯甲烷体系制备的ADN/AP复合粒子的吸湿性比原ADN降低了将近10倍.

  8. Mecanismos moleculares envolvidos na mutagenicidade induzida pela aflatoxina B1

    OpenAIRE

    2012-01-01

    La aflatoxina B1 (AFB1) es una micotoxina identificada como el más potente hepatocarcinógeno. El metabolito que resulta del proceso de detoxificación de la AFB1 en el hígado tiene la capacidad de reaccionar con el ADN genómico, generando el aducto AFB1-ADN; durante la replicación del ADN este aducto induce la transversión G:C→T:A. Polimorfismos en los genes que codifican las enzimas encargadas de la activación y detoxificación de la AFB1 y enzimas de reparación del ADN han sido asociados con ...

  9. The Edgeworth expansion for distributions of extreme values

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    We present necessary adn sufficient condition of Edgeworth expansion for drstribution sof extreme values As a corollart,artes of the uniform convergence ofr distribution s of extreme values are ovtained

  10. ClEST cluster :Cl_singleton0088 [ClEST

    Lifescience Database Archive (English)

    Full Text Available ACTGCGAAATCGAAACTCCAGTACACTTCGTTCATATTTTAC fb06016 1 peptide methionine sulfoxide reductase MsrA [Triatoma matogrossensis] ADN29867 4.31931E-63 GO:0008113 GO:0055114 GO:0006464 ...

  11. Análisis de la biodiversidad genética del algodón peruano usando marcadores moleculares: Avances en el 2004

    OpenAIRE

    2005-01-01

    Tres mini preparaciones de extracción de ADN de algodón fueron comparadas en términos de calidad y rendimiento. El método de extracción de ADN usando CTAB fue el más eficiente (30 ug) en comparación con un kit comercial de extracción (20 ug) a partir de 100 mg de hojas cotiledonarias. La óptima calidad del ADN fue evaluada con las enzimas de restricción EcoRI y MseI. El ADN preparado será usado para iniciar el análisis de la biodiversidad genética del algodón peruano, usando marcadores mole...

  12. Adiponectin and adiponectin receptor system in the rat adrenal gland: ontogenetic and physiologic regulation, and its involvement in regulating adrenocortical growth and steroidogenesis.

    Science.gov (United States)

    Paschke, Lukasz; Zemleduch, Tomasz; Rucinski, Marcin; Ziolkowska, Agnieszka; Szyszka, Marta; Malendowicz, Ludwik K

    2010-09-01

    Adiponectin (ADN) is a regulatory peptide secreted mostly by adipose tissue and acting via two receptors: AdipoR1 and AdipoR2. Our aim was to investigate expression of adiponectin system genes in the rat adrenal gland as well as its ontogenetic and physiological control. Furthermore, we examined the effects of acute and prolonged activation of HPA axis on ADN system in adipose tissue. By means of QPCR, ADN and AdipoR1 expression was demonstrated in rat adrenal cortex both at mRNA and protein levels, while AdipoR2 could only be detected at mRNA levels. ADN expression level was significantly upregulated in a developing and regenerating adrenal cortex. Globular domain of adiponectin at 10(-9) M stimulated corticosterone output and BrdU incorporation by cultured rat adrenocortical cells. Moreover, both acute (ACTH and ether stress) and prolonged (ACTH) adrenal stimulation resulted in lowered ADN levels, while expression of AdipoR1 and AdipoR2 was upregulated by the acute treatment. Depending on its site of origin, visceral (VAT) or subcutaneous (SAT) adipose tissue responded differently to alterations in HPA axis. VAT expression of ADN and its receptors remained almost unchanged by experimental manipulations. In SAT, on the other hand, expression of ADN and AdipoR2 was markedly increased by ACTH treatment and stress, while dexamethasone suppressed ADN and AdipoR1 mRNA levels. The results of this study provide new evidence for direct and indirect interactions between adipokines and HPA axis.

  13. Nursing education progression: associate degree nursing faculty perspective.

    Science.gov (United States)

    O'Neal, Dale; Zomorodi, Meg; Wagner, Jennie

    2015-01-01

    The purpose of this survey was to examine the attitudes, influences, and practices of associate degree nursing (ADN) faculty in relation to advising students about nursing education progression. The results indicated that ADN faculty have a sense of moral and personal role responsibility to encourage students to continue their education. Deficits in faculty knowledge of program details and multiple demands on their time are factors that influence advising practices.

  14. ADN的合成及性能研究(Ⅰ)%Studies on Synthesis and Properties of AND (Ⅰ)

    Institute of Scientific and Technical Information of China (English)

    王伯周; 张志忠; 朱春华; 何江涛; 雷鸣

    1999-01-01

    自行设计二硝酰胺铵(ADN)的合成方法,研究了合成ADN的关键步骤(缩合反应、二次硝化等),在国内首次合成出ADN.鉴定了ADN的结构,测定了ADN的熔点、感度、燃烧热.

  15. Adiponectin supplementation in pregnant mice prevents the adverse effects of maternal obesity on placental function and fetal growth.

    Science.gov (United States)

    Aye, Irving L M H; Rosario, Fredrick J; Powell, Theresa L; Jansson, Thomas

    2015-10-13

    Mothers with obesity or gestational diabetes mellitus have low circulating levels of adiponectin (ADN) and frequently deliver large babies with increased fat mass, who are susceptible to perinatal complications and to development of metabolic syndrome later in life. It is currently unknown if the inverse correlation between maternal ADN and fetal growth reflects a cause-and-effect relationship. We tested the hypothesis that ADN supplementation in obese pregnant dams improves maternal insulin sensitivity, restores normal placental insulin/mechanistic target of rapamycin complex 1 (mTORC1) signaling and nutrient transport, and prevents fetal overgrowth. Compared with dams on a control diet, female C57BL/6J mice fed an obesogenic diet before mating and throughout gestation had increased fasting serum leptin, insulin, and C-peptide, and reduced high-molecular-weight ADN at embryonic day (E) 18.5. Placental insulin and mTORC1 signaling was activated, peroxisome proliferator-activated receptor-α (PPARα) phosphorylation was reduced, placental transport of glucose and amino acids in vivo was increased, and fetal weights were 29% higher in obese dams. Maternal ADN infusion in obese dams from E14.5 to E18.5 normalized maternal insulin sensitivity, placental insulin/mTORC1 and PPARα signaling, nutrient transport, and fetal growth without affecting maternal fat mass. Using a mouse model with striking similarities to obese pregnant women, we demonstrate that ADN functions as an endocrine link between maternal adipose tissue and fetal growth by regulating placental function. Importantly, maternal ADN supplementation reversed the adverse effects of maternal obesity on placental function and fetal growth. Improving maternal ADN levels may serve as an effective intervention strategy to prevent fetal overgrowth caused by maternal obesity.

  16. A Biophysical Model of the Mitochondrial ATP-Mg/Pi Carrier

    OpenAIRE

    2012-01-01

    Mitochondrial adenine nucleotide (AdN) content is regulated through the Ca2+-activated, electroneutral ATP-Mg/Pi carrier (APC). The APC is a protein in the mitochondrial carrier super family that localizes to the inner mitochondrial membrane (IMM). It is known to modulate a number of processes that depend on mitochondrial AdN content, such as gluconeogenesis, protein synthesis, and citrulline synthesis. Despite this critical role, a kinetic model of the underlying mechanism has not been devel...

  17. Adiponectin Suppresses T Helper 17 Cell Differentiation and Limits Autoimmune CNS Inflammation via the SIRT1/PPARγ/RORγt Pathway.

    Science.gov (United States)

    Zhang, Kai; Guo, Yawei; Ge, Zhenzhen; Zhang, Zhihui; Da, Yurong; Li, Wen; Zhang, Zimu; Xue, Zhenyi; Li, Yan; Ren, Yinghui; Jia, Long; Chan, Koon-Ho; Yang, Fengrui; Yan, Jun; Yao, Zhi; Xu, Aimin; Zhang, Rongxin

    2016-08-11

    T helper 17 (Th17) cells are vital components of the adaptive immune system involved in the pathogenesis of most autoimmune and inflammatory syndromes, and adiponectin(ADN) is correlated with inflammatory diseases such as multiple sclerosis (MS) and type II diabetes. However, the regulatory effects of adiponectin on pathogenic Th17 cell and Th17-mediated autoimmune central nervous system (CNS) inflammation are not fully understood. In this study, we demonstrated that ADN could inhibit Th1 and Th17 but not Th2 cells differentiation in vitro. In the in vivo study, we demonstrated that ADN deficiency promoted CNS inflammation and demyelination and exacerbated experimental autoimmune encephalomyelitis (EAE), an animal model of human MS. Furthermore, ADN deficiency increased the Th1 and Th17 cell cytokines of both the peripheral immune system and CNS in mice suffering from EAE. It is worth mentioning that ADN deficiency predominantly promoted the antigen-specific Th17 cells response in autoimmune encephalomyelitis. In addition, in vitro and in vivo, ADN upregulated sirtuin 1 (SIRT1) and peroxisome proliferator-activated receptor γ (PPARγ) and inhibited retinoid-related orphan receptor-γt (RORγt); the key transcription factor during Th17 cell differentiation. These results systematically uncovered the role and mechanism of adiponectin on pathogenic Th17 cells and suggested that adiponectin could inhibit Th17 cell-mediated autoimmune CNS inflammation.

  18. Projections to the anterodorsal thalamus and lateral mammillary nuclei arise from different cell populations within the postsubiculum: implications for the control of head direction cells.

    Science.gov (United States)

    Yoder, Ryan M; Taube, Jeffrey S

    2011-10-01

    The neural representation of directional heading is encoded by a population of cells located in a circuit that includes the postsubiculum (PoS), anterodorsal thalamus (ADN), and lateral mammillary nuclei (LMN). Throughout this circuit, many cells rely on both movement- and landmark-related information to discharge as a function of the animal's directional heading. The PoS projects to both the ADN and LMN, and these connections may convey critical spatial information about landmarks, because lesions of the PoS disrupt landmark control in head direction (HD) cells and hippocampal place cells [Goodridge and Taube (1997) J Neurosci 17:9315-9330; Calton et al. (2003) J Neurosci 23:9719-9731]. The PoS → ADN projection originates in the deep layers of PoS, but no studies have determined whether the PoS → LMN projection originates from the same cells that project to ADN. To address this issue, two distinct cholera toxin-subunit B (CTB) fluorophore conjugates (Alexa Fluor 488 and Alexa Fluor 594) were injected into the LMN and ADN of the same rats, and PoS sections were examined for cell bodies containing either or both CTB conjugates. Results indicated that the PoS → LMN projection originates exclusively from a thin layer of cells located superficial to the layer(s) of PoS → ADN projection cells, with no overlap. To verify the laminar distribution and morphological characteristics of PoS → LMN and PoS → ADN cells, biotinylated dextran amine was injected into LMN or ADN of different rats, and tissue sections were counterstained with thionin. Results indicated that the PoS → LMN projection arises from large pyramidal cells in layer IV, whereas the PoS → ADN projection arises from a heterogeneous cell population in layers V/VI. This study provides the first evidence that the PoS → ADN and PoS → LMN projections arise from distinct, nonoverlapping cell layers in PoS. Functionally, the PoS may provide landmark information to HD cells in LMN.

  19. High-molecular-weight adiponectin and anthropometric variables among elementary schoolchildren: a population-based cross-sectional study in Japan

    Directory of Open Access Journals (Sweden)

    Ochiai Hirotaka

    2012-09-01

    Full Text Available Abstract Background Studies about the relationship between high-molecular-weight adiponectin (HMW-adn and anthropometric variables among population-based elementary schoolchildren have been too limited, especially in Japan, where blood collection is not usually performed in the annual health examination at elementary schools. The objective of the present study was to investigate the relationship between HMW-adn and anthropometric variables (body mass index [BMI], percent body fat [%BF], waist circumference [WC], and waist-to-height ratio [WHtR] among population-based elementary schoolchildren in Japan. Methods Subjects comprised all fourth-grade schoolchildren (9 or 10 years of age in the town of Ina, Saitama Prefecture, Japan during 2005–2008 (N = 1675. After excluding 21 subjects because of refusal to participate or incomplete data, data from a total of 1654 subjects (846 boys and 808 girls were analyzed. The height, weight, %BF, and WC of each subject were measured, while blood samples were drawn from the subjects to measure adiponectin levels (HMW-adn and total adiponectin. Childhood obesity was determined according to the age- and sex-specific cut-off points proposed by the International Obesity Task Force. Spearman’s correlation coefficients between adiponectin levels and anthropometric variables were calculated for each sex. Results The anthropometric variables were negatively correlated with HMW-adn in both boys and girls. Correlation coefficients of HMW-adn with anthropometric variables in the obesity group were consistently higher than those in the non-obesity group among both boys and girls. In addition, only WHtR was significantly correlated with HMW-adn regardless of sex and physique (obesity or non-obesity; the correlation coefficient was -0.386 among boys and -0.543 among girls in the obesity group, while it was -0.124 among boys and -0.081 among girls in the non-obesity group. Conclusions HMW-adn was negatively correlated

  20. Electrical stimulation of the aortic depressor nerve in conscious rats overcomes the attenuation of the baroreflex in chronic heart failure.

    Science.gov (United States)

    Pinto, Tomás O C Teixeira; Lataro, Renata M; Castania, Jaci A; Durand, Marina T; Silva, Carlos A A; Patel, Kaushik P; Fazan, Rubens; Salgado, Helio C

    2016-04-01

    Chronic heart failure (CHF) is characterized by autonomic dysfunction combined with baroreflex attenuation. The hypotensive and bradycardic responses produced by electrical stimulation of the aortic depressor nerve (ADN) were examined in conscious CHF and control male Wistar rats (12-13 wk old). Furthermore, the role of parasympathetic and sympathetic nervous system in mediating the cardiovascular responses to baroreflex activation was evaluated by selective β1-adrenergic and muscarinic receptor antagonists. CHF was induced by myocardial infarction. After 6 wk, the subjects were implanted with electrodes for ADN stimulation. Twenty-four hours later, electrical stimulation of the ADN was applied for 20 s using five different frequencies (5, 15, 30, 60, and 90 Hz), while the arterial pressure was recorded by a catheter implanted into the femoral artery. Electrical stimulation of the ADN elicited progressive and similar hypotensive and bradycardic responses in control (n = 12) and CHF (n = 11) rats, while the hypotensive response was not affected by methylatropine. Nevertheless, the reflex bradycardia was attenuated by methylatropine in control, but not in CHF rats. Atenolol did not affect the hypotensive or bradycardic response in either group. The ADN function was examined under anesthesia through electroneurographic recordings. The arterial pressure-ADN activity relationship was attenuated in CHF rats. In conclusion, despite the attenuation of baroreceptor function in CHF rats, the electrical stimulation of the ADN elicited a stimulus-dependent hypotension and bradycardia of similar magnitude as observed in control rats. Therefore, electrical activation of the aortic baroreflex overcomes both the attenuation of parasympathetic function and the sympathetic overdrive.

  1. Effect of deoxycorticosterone acetate-salt-induced hypertension on diabetic peripheral neuropathy in alloxan-induced diabetic WBN/Kob rats.

    Science.gov (United States)

    Ozaki, Kiyokazu; Hamano, Hiroko; Matsuura, Tetsuro; Narama, Isao

    2016-01-01

    The relationship between hypertension and diabetic peripheral neuropathy (DPN) has recently been reported in clinical research, but it remains unclear whether hypertension is a risk factor for DPN. To investigate the effects of hypertension on DPN, we analyzed morphological features of peripheral nerves in diabetic rats with hypertension. Male WBN/Kob rats were divided into 2 groups: alloxan-induced diabetic rats with deoxycorticosterone acetate-salt (DOCA-salt) treatment (ADN group) and nondiabetic rats with DOCA-salt treatment (DN group). Sciatic, tibial (motor) and sural (sensory) nerves were subjected to qualitative and quantitative histomorphological analysis. Systolic blood pressure in the two groups exhibited a higher value (>140 mmHg), but there was no significant difference between the two groups. Endoneurial blood vessels in both groups presented endothelial hypertrophy and narrowing of the vascular lumen. Electron microscopically, duplication of basal lamina surrounding the endothelium and pericyte of the endoneurial vessels was observed, and this lesion appeared to be more frequent and severe in the ADN group than the DN group. Many nerve fibers of the ADN and DN groups showed an almost normal appearance, whereas morphometrical analysis of the tibial nerve showed a significant shift to smaller fiber and myelin sizes in the ADN group compared with DN group. In sural nerve, the fiber and axon-size significantly shifted to a smaller size in ADN group compared with the DN group. These results suggest that combined diabetes and hypertension could induce mild peripheral nerve lesions with vascular changes.

  2. Operation Optimization Based on the Power Supply and Storage Capacity of an Active Distribution Network

    Directory of Open Access Journals (Sweden)

    Wenpeng Yu

    2013-12-01

    Full Text Available Due to the interconnection and active management of Distributed Generation (DG and Energy Storage Systems (ESSs, the traditional electrical distribution network has become an Active Distribution Network (ADN, posing challenges to the operation optimization of the network. The power supply and storage capacity indexes of a Local Autonomy Control Region (LACR, which consists of DGs, ESSs and the network, are proposed in this paper to quantify the power regulating range of a LACR. DG/ESS and the network are considered as a whole in the model of the indexes, considering both network constraints and power constraints of the DG/ESS. The index quantifies the maximum LACR power supplied to or received from ADN lines. Similarly, power supply and storage capacity indexes of the ADN line are also proposed to quantify the maximum power exchanged between ADN lines. Then a practical algorithm to calculate the indexes is presented, and an operation optimization model is proposed based on the indexes to maximum the economic benefit of DG/ESS. In the optimization model, the power supply reliability of the ADN line is also considered. Finally, the indexes of power supply and storage capacity and the optimization are demonstrated in a case study.

  3. ISOLATION OF GENOMIC DNAs FROM THE TROPICAL FRUIT TREES AVOCADO, COCONUT, GUAVA AND MANGO FOR PCR-BASED DNA MARKER APPLICATION

    Directory of Open Access Journals (Sweden)

    Isis M. Ramírez

    2004-01-01

    Full Text Available En frutales tropicales de porte arbóreo, el aisla- miento del ADN genómico con suficiente calidad para ser usa- do en tecnologías de marcadores moleculares basadas en PCR, posee en muchas ocasiones serios problemas por la presencia de inhibidores, tales como los polisacáridos que inhiben el procesamiento enzimático del ADN o los polifenoles que inhiben las reacciones de PCR. Se probaron diferentes proto- colos de extracción y purificación de ADN en cuatro frutales tropicales: guayabo ( Psidium guajava L., aguacatero ( Persea americana Mill., mango ( Mangifera indica L. y cocotero ( Cocos nucifera L.. El protocolo de Doyle y Doyle logró buenos rendimientos y calidad del ADN para la amplificación (PCR en mango y cocotero, pero no en guayaba y aguacatero. Para estas dos últimas especies se probaron diferentes técni- cas con resultados igualmente insatisfactorios. La modifica- ción del método del CTAB con respecto a la composición del buffer de extracción en combinación con el empleo de colum- nas de adsorción reversible NucleoSpin, permitió la extracción exitosa del ADN con la calidad suficiente para aplicar las técni- cas de AFLP, SSR e ISTR en guayabo y/o aguacatero.

  4. Evaluation of the radiosensitizing to treatment with {sup 153}Sm-EDTMP, of haematopoietic cells of the bone marrow by means of bromodeoxyuridine incorporation into DNA, in a murine model; Evaluacion de la radiosensibilizacion al tratamiento con {sup 153}Sm-EDTMP, de las celulas hemotopoyeticas de la medula osea mediante la incorporacion de bromodesoxiuridina (BrdU) en el ADN, en un modelo murino

    Energy Technology Data Exchange (ETDEWEB)

    Morales A, E.

    2008-07-01

    Bromodeoxyuridine (BrdU) has been shown to have a radiosensitizing effect, and its incorporation into DNA prior to administration of a bone-seeking radiopharmaceutical could increase the efficiency of bone marrow ablation, and even increase the specificity of radiation exposure for therapeutic purposes. The aim of the present study was to determine the effect of BrdU incorporation into DNA on the genotoxic and cytotoxic effects of samarium-153 ethylenediaminetetra-methylene phosphonate ({sup 153}Sm-EDTMP) in murine bone marrow cells. BALB/c male mice (N = 5 in each experiment) were treated with one of the following substances: a) BrdU (0.25 mg/g) b) {sup 153}-EDTMP (11.5 +- 3 MBq) c) BrdU (0.25 mg/g) plus {sup 153}Sm-EDTMP (11.5 +- MBq), there was also an untreated control. Cytotoxicity and genotoxicity were established by time-response and absorbed dose-response curves of polychromatic erythrocyte (PCE) and micro nucleated polychromatic erythrocyte (MN-PCE) frequencies, respectively, in murine peripheral blood samples in vivo. The significance of the differences between groups was determined by a variation of Dunett test for multiple groups and different-sized groups of a student test. Beta-absorbed dose fractions obtained from MNCP4B Monte Carlo computer code were used for mice bone marrow dosimetry calculations. At an average radiation absorbed dose of 0.38 Gy, 0.56 Gy and 0.82 Gy at 24, 40 and 72 h respectively, cells from animals treated with {sup 153}Sm-EDTMP showed a clear and significant induction of MN-PCE after 24 h, with the maximum response at 40 h, however, cells from group treated with BrdU plus {sup 153}Sm-EDTMP paradoxically showed MN-PCE frequencies only slightly higher than the control at the same absorbed dose. Treatment with {sup 153}Sm-EDTMP caused a slight reduction in PCE frequency, but exposure to BrdU or BrdU plus {sup 153}Sm-EDTMP induced a substantial and significant reduction in PCE frequency from 32 h to the end of the experiment (72 h). The PCE frequencies in the BrdU pretreated and irradiated group were consistently lower than in the BrdU control group, and the difference was significant at the final scored times in the experiment. These effects could be explained by lethal lesions of the accumulation of sublethal damage induced by the irradiation of BrdU-substituted DNA, where this lesion is not easily repaired and subsequently does not permit cell division or micronucleus formation. This research shows the increasing of cytotoxicity when BrdU was administered before the {sup 153}Sm-EDTMP treatment. Further studies are required in order to explore the utility of this strategy for bone marrow ablation or therapy in humans. (Author)

  5. Rocket Solid Propellant Alternative Based on Ammonium Dinitramide

    Directory of Open Access Journals (Sweden)

    Grigore CICAN

    2017-03-01

    Full Text Available Due to the continuous run for a green environment the current article proposes a new type of solid propellant based on the fairly new synthesized oxidizer, ammonium dinitramide (ADN. Apart of having a higher specific impulse than the worldwide renowned oxidizer, ammonium perchlorate, ADN has the advantage, of leaving behind only nitrogen, oxygen and water after decomposing at high temperatures and therefore totally avoiding the formation of hydrogen chloride fumes. Based on the oxidizer to fuel ratios of the current formulations of the major rocket solid booster (e.g. Space Shuttle’s SRB, Ariane 5’s SRB which comprises mass variations of ammonium perchlorate oxidizer (70-75%, atomized aluminum powder (10-18% and polybutadiene binder (12-20% a new solid propellant was formulated. As previously stated, the new propellant formula and its variations use ADN as oxidizer and erythritol tetranitrate as fuel, keeping the same polybutadiene as binder.

  6. Hiring Intentions of Directors of Nursing Programs Related to DNP- and PhD-Prepared Faculty and Roles of Faculty.

    Science.gov (United States)

    Oermann, Marilyn H; Lynn, Mary R; Agger, Charlotte A

    2016-01-01

    This study surveyed administrators of associate degree in nursing (ADN) and bachelor of science in nursing (BSN) programs across the United States to identify hiring intentions and describe the roles and responsibilities of DNP- and PhD-prepared faculty members. The final sample included 253 ADN and 229 BSN programs. ADN programs were neither intentionally hiring nor looking to hire doctorally prepared nurse faculty. Deans and directors of BSN programs reported an average of 3 openings for the next academic year, 2 projected for new PhD-prepared faculty and 1 for a faculty member with a DNP. Schools have made varying decisions regarding the type of appointment (tenure or nontenure track) for DNP-prepared faculty members. Challenges that DNP-prepared faculty members encountered in meeting the role and promotion expectations in their schools focused predominantly on scholarship.

  7. PfGBP: una proteína de unión al telómero de Plasmodium falciparum

    Directory of Open Access Journals (Sweden)

    Eliana Calvo

    2015-01-01

    Full Text Available Los telómeros son estructuras complejas de ADN y proteína localizadas en el extremo de los cromosomas eucariotes. Su principal función es proteger el extremo cromosomal de ser reconocido y procesado como ADNs fracturado, evitando así eventos de recombinación y fusión que conducen a inestabilidad cromosomal. El ADN telomérico consta de secuencias cortas, repetidas una tras otra, ricas en guanina; la cadena rica en guanina se extiende formando una región de cadena sencilla denominada extremo 3´ protuberante. Las proteínas por su parte, se pueden clasificar en: dsBPs, o proteínas de unión a la cadena doble, GBPs aquellas que reconocen específicamente el extremo protuberante y, proteínas que las interconectan mediante interacciones proteína-proteína. El gen PF3D7_1006800 de Plasmodium falciparum codifica para una proteína putativa similar a una GBP de Criptosporidium parvum, con el fin de establecer si esta proteína de P. falciparum presenta la capacidad de unión al ADN telomérico del parásito, se produjo una proteína recombinante a partir de la región codificante del gen, se purificó y se utilizó en ensayos de unión a ADN, y en la generación de anticuerpos policlonales específicos contra PfGBP. Nuestros resultados indican que la proteína de P. falciparum es una proteína nuclear con capacidad de unión al ADN telomérico in vitro, por lo que podría ser parte del complejo proteico encargado de proteger y/o mantener el telómero in vivo.

  8. Adenosine conjugated lipidic nanoparticles for enhanced tumor targeting.

    Science.gov (United States)

    Swami, Rajan; Singh, Indu; Jeengar, Manish Kumar; Naidu, V G M; Khan, Wahid; Sistla, Ramakrishna

    2015-01-01

    Delivering chemotherapeutics by nanoparticles into tumor is impeded majorly by two factors: nonspecific targeting and inefficient penetration. Targeted delivery of anti-cancer agents solely to tumor cells introduces a smart strategy because it enhances the therapeutic index compared with untargeted drugs. The present study was performed to investigate the efficiency of adenosine (ADN) to target solid lipid nanoparticles (SLN) to over expressing adenosine receptor cell lines such as human breast cancer and prostate cancer (MCF-7 and DU-145 cells), respectively. SLN were prepared by emulsification and solvent evaporation process using docetaxel (DTX) as drug and were characterized by various techniques like dynamic light scattering, differential scanning calorimeter and transmission electron microscopy. DTX loaded SLNs were surface modified with ADN, an adenosine receptors ligand using carbodiimide coupling. Conjugation was confirmed using infrared spectroscopy and quantified using phenol-sulfuric acid method. Conjugated SLN were shown to have sustained drug release as compared to unconjugated nanoparticles and drug suspension. Compared with free DTX and unconjugated SLN, ADN conjugated SLN showed significantly higher cytotoxicity of loaded DTX, as evidenced by in vitro cell experiments. The IC50 was 0.41 μg/ml for native DTX, 0.30 μg/ml for unconjugated SLN formulation, and 0.09 μg/ml for ADN conjugated SLN formulation in MCF-7 cell lines. Whereas, in DU-145, there was 2 fold change in IC50 of ADN-SLN as compared to DTX. IC50 was found to be 0.44 μg/ml for free DTX, 0.39 μg/ml for unconjugated SLN and 0.22 μg/ml for ADN-SLN. Annexin assay and cell cycle analysis assay further substantiated the cell cytotoxicity. Fluorescent cell uptake and competitive ligand-receptor binding assay corroborated the receptor mediated endocytosis pathway indicated role of adenosine receptors in internalization of conjugated particles. Pharmacokinetic studies of lipidic

  9. Los factores de transcripción tipo Myb, una familia de reguladores de la diferenciación celular conservada en los organismos eucariontes

    OpenAIRE

    Jenny Arratia; Jesús Aguirre

    2013-01-01

    La familia de proteínas Myb, ubicua en los eucariontes, se caracteriza por la presencia de un dominio de unión al ADN característico denominado dominio Myb. Éste consiste en una secuencia de aminoácidos conservados (50-53 aminoácidos) que puede estar repetida entre dos (dominio mínimo de unión al ADN) y hasta cuatro veces en la misma proteína. En las plantas, la familia Myb es muy numerosa, mientras que en los animales sólo se encuentran tres miembros, y en otros eucariontes se ha identificad...

  10. Genética y genómica enfocadas en el estudio de la resistencia bacteriana

    OpenAIRE

    Ulises Garza-Ramos; Jesús Silva-Sánchez; Esperanza Martínez-Romero

    2009-01-01

    La resistencia bacteriana es un problema de salud pública causante de índices elevados de morbi-mortalidad hospitalaria. En la medida en que se usan los diferentes antibióticos se seleccionan bacterias resistentes a múltiples fármacos. El desarrollo de nuevas herramientas moleculares de la genómica y proteómica, como el PCR en tiempo real, pirosecuenciación de ADN, espectrometría de masas, microarreglos de ADN y bioinformática, permite conocer en forma más estrecha la fisiología y estructura ...

  11. TRANSFORMACIÓN DE PLANTAS MEDIADA POR AGROBACTERIUM: "INGENIERÍA GENÉTICA NATURAL APLICADA" PLANT TRANSFORMATION MEDIATED BY AGROBACTERIUM: "APPLIED NATURAL GENETIC ENGINEERING"

    OpenAIRE

    Ana Milena Valderrama Fonseca; Rafael Arango Isaza; Lucia Afanador Kafuri

    2005-01-01

    Agrobacterium tumefaciens tiene la capacidad de transferir ADN entre reinos diferentes. El impacto de este hallazgo ha tenido grandes aplicaciones en diversos campos de la biología vegetal, agricultura y biotecnología. En este artículo se describen los procesos por los cuales Agrobacterium realiza la transferencia de ADN a la planta, puntualizando en 7 eventos fundamentales para la interacción A. tumefaciens-planta y esta dirigido a profesionales de las áreas biológicas que estén interesados ...

  12. The epigenetic regulation of cell cycle and chromatin dynamic by sirtuins

    OpenAIRE

    Martínez Redondo, Paloma

    2014-01-01

    La cromatina consiste en una estructura jerárquica y dinámica que se regula durante el ciclo celular con el fin de mantener la integridad del genoma y preservar la información genética codificada en el ADN. Esta estructura dinámica depende de la coordinación de diferentes procesos: modificaciones histónicas, la actividad de enzimas/complejos remodeladores de la cromatina, metilación del ADN y la participación de proteínas estructurales de la cromatina. De entre estos procesos, las modificaci...

  13. 含分布式电源和微电网的主动配电网设计方法研究

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    为了应对化石能源枯竭和气候变化所带来的巨大挑战,包含了分布式电源(DG)和微电网(MG)的主动配电网(ADN)概念应运而生.作为一项新技术, (ADN)的规划设计关系到系统的运行成效,因此本文对其设计方法进行了研究.

  14. Avago推出两款超低功耗光学传感器

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Avago Technologies日前宣布推出两个最新的用于无线鼠标的低功率光学传感器。ADNS-2080和ADNS-3000LED传感器利用先进的低功率架构和自动电源管理模式,使无线鼠标在只使用一枚AA电池的情况下可以操作长达一年之久。

  15. 主动配电系统可行技术的研究%Enabling Technologies for Active Distribution Systems

    Institute of Scientific and Technical Information of China (English)

    范明天; 张祖平; 苏傲雪; 苏剑

    2013-01-01

    The planning methods and operation modes of traditional distribution network are difficult to accommodate the high penetration of distributed energy resources (DER). Active distribution network (ADN), based on the application of information and communication technology (ICT) and advanced metering infrastructure (AMI), is one of the alternative solutions. In this paper, the impact of DER on the traditional distribution network was analyzed and thus the necessity of developing ADN was clarified;The basic concept of ADN, its research tendency, the differences between traditional distribution networks and ADN and the corresponding research results were introduced;Some enabling technologies which are cost-effective for the transitions from the existing distribution network to the active distribution network were discussed. With the progress of the available techniques, ADN can balance the power at distribution levels, and between transmission and distribution levels, that is, ADN will be an infrastructure as a regional exchange of all kinds of energy.%  传统配电网的规划设计方法和运行控制模式无法适应高渗透率分布式能源(distributed energy resources,DER)的接入。基于信息与通信技术及高级量测设备的主动配电网(active distribution network,ADN)为高渗透率DER的接入提供了一种解决方案。概要分析DER接入对传统配电网的影响,以此说明发展ADN的必要性;介绍ADN的基本概念、相关的研究动态、传统配电网与主动配电网的差异及研究成果;基于技术经济可行性的观点,总结适应ADN发展的可行技术。随着各种可行技术的进步,ADN 不仅可以平衡配电层级的功率,还可平衡配电层级与上级电网之间的功率,ADN将成为局部区域能源交换的基础设施。

  16. New Replacement Policy on Skewed-associative TLB%一种适用于Skewed相联TLB的替换算法

    Institute of Scientific and Technical Information of China (English)

    菅陆田; 唐秀存

    2006-01-01

    Skewed相联方式虽然能够比组相联更好地解决TLB冲突脱靶问题,但在Skewed相联TLB上实现传统的LRU替换算法硬件代价太大,因此,本文介绍了一种适用于Skewed相联TLB的替换算法--ADN-NRU,并对实现ADN-NRU替换算法的Skewed相联TLB进行了性能测试.

  17. Genética y genómica enfocadas en el estudio de la resistencia bacteriana Genetics and Genomics for the study of bacterial resistance

    OpenAIRE

    Ulises Garza-Ramos; Jesús Silva-Sánchez; Esperanza Martínez-Romero

    2009-01-01

    La resistencia bacteriana es un problema de salud pública causante de índices elevados de morbi-mortalidad hospitalaria. En la medida en que se usan los diferentes antibióticos se seleccionan bacterias resistentes a múltiples fármacos. El desarrollo de nuevas herramientas moleculares de la genómica y proteómica, como el PCR en tiempo real, pirosecuenciación de ADN, espectrometría de masas, microarreglos de ADN y bioinformática, permite conocer en forma más estrecha la fisiología y estructura ...

  18. Estudio estructural de complejos de oligonucleótidos ricos en adenina y timina con la proteína HMGB1

    OpenAIRE

    García Gómez, Sonia

    2015-01-01

    La familia de proteínas HMGB pertenece a la superfamilia de las proteínas HMG (High Mobility Group), que son las proteínas más abundantes en la cromatina después de las histonas. Las HMGB se unen al surco estrecho del ADN (ácido desoxirribonucleico) con poca o ninguna especificidad de secuencia, a partir de un motivo de unión al ADN denominado HMG-box. Existen dos dominios de HMG-box en las proteínas HMGB, las llamadas box A y box B. Cada una de ellas contiene aproximadamente 75 aminoácidos y...

  19. Terapia génica dirigida en una nuevo "sitio seguro" en células progenitoras hematopoyéticas humanas para su aplicación en anemia de Fanconi

    OpenAIRE

    Rodríguez Fornés, Fátima

    2016-01-01

    La anemia de Fanconi es una enfermedad hereditaria de baja prevalencia, descrita por primera vez por el pediatra Guido Fanconi en 1927. Esta enfermedad se produce como consecuencia de mutaciones en cualquiera de los 19 genes de Fanconi descritos hasta la actualidad, y que participan en la ruta de Fanconi/BRCA. Esta ruta se encarga de la reparación de enlaces intercatenarios del ADN y de coordinar los distintos mecanismos de reparación de las dobles roturas en el ADN. La anemia de Fanconi está...

  20. Consecuencias epistémicas y ontológicas para la definición de Gen y de Dogma Central de la Biología Molecular derivadas de las nuevas técnicas de secuenciación masiva en la Etapa Post-Genómica

    OpenAIRE

    Martínez Gómez, Pedro

    2015-01-01

    [ES] En estos momentos, en la llamada Etapa Post-Genómica, nos encontramos ante una nueva revolución biológica similar a la originada con el descubrimiento de la estructura del ADN y el desarrollo de la Genética Molecular. Esta nueva Etapa se caracteriza desde un punto de vista epistémico y metodológico por la incorporación de nuevos métodos de secuenciación tanto de ADN como de ARN y por el desarrollo de genomas que permiten referenciar de una forma precisa los resultados moleculares obtenid...

  1. Transformación de plantas mediada por agrobacterium: “ingeniería genética natural aplicada”

    OpenAIRE

    Valderrama, Ana Milena

    2011-01-01

    Agrobacterium tumefaciens tiene la capacidad de transferir ADN entre reinos diferentes. El impacto de este hallazgo ha tenido grandes aplicaciones en diversos campos de la biología vegetal, agricultura y biotecnología. En este artículo se describen los procesos por los cuales Agrobacterium realiza la transferencia de ADN a la planta, puntualizando en 7 eventos fundamentales para la interacción A. tumefaciens-planta y esta dirigido a profesionales de las áreas biológicas que estén interesados...

  2. Identificación de cadáveres sometidos a altas temperaturas, a partir de las características macroscópicas de sus órganos dentales y la aplicabilidad de la genética forense / Identification of Bodies Exposed to High Temperatures Based on Macroscopic...

    OpenAIRE

    Barraza Salcedo, María del Socorro; Universidad Metropolitana de Barranquilla. Barranquilla; Rebolledo Cobos, Martha Leonor; Universidad Metropolitana de Barranquilla

    2016-01-01

    RESUMEN. Antecedentes: La odontología forense en los casos de incineración suministra elementos científicos que permiten la identificación de cadáveres, mediante el análisis de órganos dentales, a través del aislamiento de ADN obtenido de la pulpa como alternativa para confirmar la identidad de la víctima. Cuando el grado de temperatura es altamente elevado, los tejidos dentales son vulnerables y, por ende, el ADN pulpar no es rescatable, perdiendo recursos y tiempo por la carencia de estánda...

  3. Aplicación de la técnica de PCR (Reacción en cadena de la polimerasa) en el diagnóstico y epidemiología de la tuberculosis en animales

    OpenAIRE

    Aranaz Martín, Alicia

    2012-01-01

    El trabajo describe el desarrollo de un método de extracción del ADN de micobacterias a partir de muestras clínicas. El ADN obtenido se amplifico mediante pcr con iniciadores específicos de genero y especie o complejo. Respecto al cultivo, la sensibilidad de los protocolos fue del 90,6% (muestras de vacuno, caprino, ciervos y jabalíes) y del 100% (perros, gatos y aves). Esta técnica reduce el tiempo necesario para el diagnostico a 2 o 3 días. Al evaluar la técnica random amplificación polymor...

  4. Evidencia genética revela oportunidades de mejora para preparación de tejidos en análisis forenses

    OpenAIRE

    Romero, Rosa Elena; Sandoval, Alejandro; Arango, Juliana; Camargo, Martha Lucia

    2016-01-01

    Introducción. Los tejidos embebidos en parafina constituyen una excelente alternativa para obtener ADN, especialmente cuando no es posible contar con muestras frescas o porque el almacenamiento y conservación del tejido no es viable; haciendo de esta muestra el único elemento disponible para realizar un cotejo. El éxito en cualquier análisis genético es contar con métodos de fijación de tejidos y de extracción de ADN adecuados que permitan obtener moléculas de buena calidad y cantidad, libres...

  5. Comparación de la frecuencia alélica de 13 loci STRs de la población brasileña y española para fines de identificación humana en genética forense

    OpenAIRE

    Rigotti, Juliana Filho

    2013-01-01

    Los marcadores microsatélites son elementos extraordinariamente útiles en la identificación humana y en el mapeo genético. Además, se distribuyen ampliamente por todo el genoma y son valiosos en los casos donde existe la necesidad de analizar huesos, cabellos, manchas de sangre y otros materiales en los cuales podemos encontrar el ADN degradado o en pequeñas cantidades. La prueba del ADN constituye, actualmente, una pericia de enorme transcedencia en muchos casos judiciales, lo que ha supuest...

  6. Navy Network Dependability: Models, Metrics, and Tools

    Science.gov (United States)

    2010-01-01

    surveillance; TCDL = Tactical Common Data Link; UHF = ultra high frequency; UFO = ultra-high-frequency follow-on; WGS = Wideband Gapfiller Satellite. RAND...VOICE (DMR VALUES) UFO OE-82 UHF LOS VOICE (DMR VALUES) UHF SATCOM VOICE DMR UHF LOS VOICE DMR TVs ADMS KIV-7 COMSEC ADNS SW ADNS II HW ISNS SW ISNS HW...Data Link; UHF = ultra high frequency; UFO = ultra-high-frequency follow-on; WGS = Wideband Gapfiller Satellite. RAND MG1003-1.1 4 Navy Network

  7. Proteomic analysis of HIV-T cell interaction: an update.

    Directory of Open Access Journals (Sweden)

    Dave eSpeijer

    2012-07-01

    Full Text Available This mini-review summarizes techniques applied in, and results obtained with, proteomic studies of HIV-1 T cell interaction. Our group previously reported on the use of two-dimensional differential gel electrophoresis (2D-DIGE coupled to MALDI-TOF peptide mass fingerprint analysis, to study T cell responses upon HIV-1 infection. Only one in three differentially expressed proteins could be identified using this experimental setup. Here we report on our latest efforts to test models generated by this data set and extend its analysis by using novel bioinformatic algorithms. The 2D-DIGE results are compared with other studies including a pilot study using one-dimensional peptide separation coupled to MSE, a novel mass spectrometric approach. It can be concluded that although the latter method detects fewer proteins, it is much faster and less labor intensive. Last but not least, recent developments and remaining challenges in the field of proteomic studies of HIV-1 infection and proteomics in general are discussed.

  8. 原子层沉积技术合成氧化铝薄膜包覆二硝酰胺铵%Alumina Thin Film Coated Ammonium Dinitramide Fabricated by Atomic Layer Deposition

    Institute of Scientific and Technical Information of China (English)

    龚婷; 秦利军; 严蕊; 胡岚; 姬月萍; 冯昊

    2014-01-01

    通过原子层沉积(ALD)技术以三甲基铝和水作为前驱体在二硝酰胺铵(ADN)表面沉积氧化铝包覆膜。分别采用扫描电子显微镜(SEM), X射线光电子能谱(XPS)对包覆后ADN的表面形貌、化学成分进行了分析,通过蒸汽吸附分析仪(VSA)对包覆氧化铝薄膜的ADN样品进行了吸湿性测试,并且对ADN表面氧化铝薄膜生成机理进行了探讨。结果表明:ALD氧化铝薄膜对ADN表面形成了完整的包覆,薄膜厚度最高可达数百纳米。包覆有ALD氧化铝薄膜的ADN样品暴露在潮湿空气中48 h形貌不发生明显变化。在25℃,湿度70%的环境条件下, VSA测得包覆200和400周期氧化铝薄膜的ADN吸湿率分别为40.99%和40.75%。以上研究结果表明,尽管ALD氧化铝对ADN表面实现了完整包覆并在潮湿空气中维持了样品形貌,被包覆的ADN样品吸湿性尚未获得明显改善。%To deposit alumina thin films on ammonium dinitramide (ADN) by atomic layer deposition (ALD), trimethylaluminum and water were used as the precursors. The surface morphology and chemical compositions of the ALD alumina coated ADN were characterized by scanning electron microscope (SEM) and X-ray photoelectron spec-troscope (XPS). The hygroscopic property of the ALD alumina coated ADN was studied by a vapor adsorption ana-lyzer (VSA). The possible mechanism of ALD alumina film growth on the surface of ADN was discussed. The charac-terization results indicate that the ALD alumina film completely covers the surface of ADN. The thickness of the alu-mina film can reach hundreds of nanometers. After 48 h of air exposure, the shapes and topologies of the alumina coated ADN particles are maintained. The hygroscopicity of the ADN samples coated by 200 and 400 cycles of ALD alumina are 40.99%and 40.75%, respectively. Although the ALD alumina coating completely covers the surface of ADN and successfully maintains the shapes and topologies of ADN particles in a wet environment

  9. Adiponitrile-LiTFSI solution as alkylcarbonate free electrolyte for LTO/NMC Li-ion batteries.

    Science.gov (United States)

    Farhat, Douaa; Ghamouss, Fouad; Maibach, Julia; Edström, Kristina; Lemordant, Daniel

    2017-02-23

    Recently, dinitriles (NC(CH2)nCN) and especially adiponitrile (ADN, n=4) have attracted the attention as secure electrolyte solvents due to their chemical stability, high boiling points, high flash points and low vapor pressure. The good solvating properties of ADN toward lithium salts and its high electrochemical stability (~ 6V vs. Li/Li+) make it suitable for safer Li-ions cells without performances loss. In this study, ADN is used as a single electrolyte solvent with lithium bis(trimethylsulfonyl)imide (LiTFSI). This electrolyte allows the use of aluminum collectors as almost no corrosion occurs at voltages up to 4.2 V. Physico-chemical properties of ADN-LiTFSI electrolyte such as salt dissolution, conductivity and viscosity were determined. The cycling performances of batteries using Li4Ti5O12 (LTO) as anode and LiNi1/3Co1/3Mn1/3O2 (NMC) as cathode were determined. The results indicate that LTO/NMC batteries exhibit excellent rate capabilities with a columbic efficiency close to 100%. As an example, cells were able to reach a capacity of 165 mAh.g-1 at 0.1C and a capacity retention of more than 98% after 200 cycles at 0.5C. In addition, electrodes analyses by SEM, XPS and electrochemical impedance spectroscopy after cycling confirming minimal surface changes of the electrodes in the studied battery system.

  10. Main: ABREDISTBBNNAPA [PLACE

    Lifescience Database Archive (English)

    Full Text Available ABREDISTBBNNAPA S000262 16-Feb-2001 (last modified) seki dist B (distal portion of ...equired for seed specific expression and ABA responsiveness; See S000263, S000264; dist B ABRE mediated tran...sactivation by ABI3 adn ABI3-dependent response to ABA; a tetramer of the composite RY/G complex medi

  11. Amplificación del gen hsp18 para la detección de Mycobacterium leprae

    Directory of Open Access Journals (Sweden)

    Róger Calderón E

    2006-10-01

    Full Text Available Mediante PCR se amplificó un fragmento de 360 pb del gen hsp18, que codifica al antígeno proteico de 18kDa de M. leprae, a partir de una biopsia de un paciente con diagnóstico baciloscópico, histopatológico y clínico de lepra. Además, se evaluaron tejidos embebidos en parafina (fijados en formol y ADN de otras micobacterias para determinar la especificidad, sensibilidad y confiabilidad del método. El ensayo de PCR amplificó clara y satisfactoriamente ADN de M. leprae procedente de la biopsia del paciente, pero fue incapaz de amplificar usando ADN purificado a partir de tejidos embebidos en parafina. No se observaron productos de amplificación al utilizar ADN genómico de varias micobacterias tales como M. tuberculosis, M. bovis, M. fortuitum, M. gordonae, M. kansasii, M. scrofulaceum, M. avium entre otras, así como de otras bacterias. Este sistema puede considerarse como una alternativa para la identificación de pacientes infectados con M. leprae orientando el esfuerzo para determinar la prevalencia oculta de la enfermedad mediante la identificación de casos asintomáticos con capacidad de transmisión de bacilos.

  12. 10 años de la secuenciación del genoma humano: encuentro entre el imaginario y la realidad

    OpenAIRE

    María Teresa Reguero Reza

    2013-01-01

    Lejano parece en año de 1953 cuando se publica en Nature un artículo sobre la estructura helicoidal de ADN, propuesta por dos investigadores, el británico Francis Crick y el estadounidense James Watson, lo que constituyó la piedra angular de los procesos de secuenciación.

  13. Transition from Associate's Degree in Nursing to Bachelor's of Science in Nursing

    Science.gov (United States)

    Allar, Deborah T.

    2014-01-01

    Areas throughout the United States lack baccalaureate-prepared registered nurses to meet the health care needs of individuals, forcing health care providers to rely on associate degree nurses (ADN). In an effort to increase the numbers of Bachelor of Science in Nursing (BSN) students, technical colleges and state and private universities have…

  14. Método de diagnóstico y/o pronóstico de enfermedades neurodegenerativas

    OpenAIRE

    Trullás Oliva, Ramón; Figueiro-Silva, Joana; Mihaylovich Podlesniy, Petar

    2012-01-01

    [ES] La presente invención se refiere al uso del ADN mitocondrial como biomarcador cuantitativo de enfermedades neurodegenerativas, preferiblemente de la enfermedad de Alzheimer, así como a un método y a un kit diagnóstico y/o pronóstico de dichas enfermedades mediante el uso de dicho biomarcador

  15. Método de diagnóstico y/o pronóstico de enfermedades neurodegenerativas

    OpenAIRE

    Trullás Oliva, Ramón; Figueiro-Silva, Joana; Mihaylovich Podlesniy, Petar

    2012-01-01

    La presente invención se refiere al uso del ADN mitocondrial como biomarcador cuantitativo de enfermedades neurodegenerativas, preferiblemente de la enfermedad de Alzheimer, así como a un método y a un kit diagnóstico y/o pronóstico de dichas enfermedades mediante el uso de dicho biomarcador

  16. Examining Harasim's Online Collaborative Learning Theory for Nursing Education

    Science.gov (United States)

    Breen, Henny

    2013-01-01

    Online nursing education has been evolving at a rapid pace as it is recognized as offering the flexibility needed for practicing associate degree (ADN) and diploma prepared Registered Nurses to return to school to earn their BSN. At the same time, there is a paradigm shift in how nursing education is delivered. The focus has shifted from content…

  17. Caracterización genética molecular de materiales procesados de Stevia rebaudiana utilizando la técnica de microsatélites

    Directory of Open Access Journals (Sweden)

    Giovanni Garro-Monge

    2014-09-01

    En este estudio se analizó la amplificación de los ISSR S3, S4, S5, S10, S11, S12 y S15 a partir de muestras de ADN de S. rebaudiana, provenientes de plantas cultivadas en invernadero. Las muestras de ADN se obtuvieron mediante tres diferentes protocolos de extracción a partir de material seco y fresco de la planta. De acuerdo con los resultados obtenidos, los ISSR S3, S4, S5 y S11 representan una buena opción para la detección de polimorfismos en esta especie, y por tanto para la identificación molecular de la especie. Se debe comprobar si los ISSR S10, S12 y S15 son capaces de amplificar segmentos de ADN de Stevia, ya que bajo las condiciones de trabajo de esta investigación no se logró la amplificación de ADN con estos imprimadores. Se considera que los ISSR son marcadores universales y se podría identificar individualmente cada cultivar de Stevia que vaya a destinarse a la producción comercial de esteviósidos, pero es necesario repetir los protocolos descritos con diferentes variedades e incluso se puede trabajar con diferentes especies del género Stevia, para obtener una caracterización general.

  18. Ketogenesis in rat-liver mitochondria: Stimulation by palmityl-coenzyme A

    NARCIS (Netherlands)

    Vaartjes, W.J.; Lopes-Cardozo, M.; Bergh, S.G. van den

    1972-01-01

    It is well-known that the movement of adenine nucleotides (AdN) across the inner mitochondrial membrane is markedly decreased both by unsaturated and by saturated long-chain fatty acids. A similar effect is displayed by palmityl-CoA as demonstrated recently with isolated mitochondria of rat heart an

  19. Modigliani-Miller, Basel 3 and CRD 4

    DEFF Research Database (Denmark)

    Balling, Morten

    2015-01-01

    Since 2007, bank capital regulation has been strengthened in Europe and globally. Bank organizations have expressed serious concerns about the impact of higher capital requirements on bank funding costs adn on the lending capacity of banks. The message of a 1958-Article by Modigliani and Miller...

  20. A Vibration Offset Collection Equipment Based on the Sensor Technology of Photoelectric Mouse*%一种基于光电鼠标传感技术的振动偏移量采集设备

    Institute of Scientific and Technical Information of China (English)

    孙宁; 陈进; 林立新

    2011-01-01

    阐述了基于CY7C68013和ADNS-3080的两自由度振动偏移量实时采集设备的设计与实现.详细分析了采用ADNS-3080完成图像采集、分析,并得出最终有效数据△x和△y值的方法;采用CY7C68013进行数据处理并通过SPI总线和USB总线进行数据传输的过程;以及上位机的数据存储、分析、显示和网络传输的软件设计.%The paper described the design and implementation of the real-time collection equipment for 2-D of vibration offset based on the CY7C68013 and the ADNS-3080. It analysed the method for image acquisition, analysis, and reached the final valid data of values △χ and △y by ADNS-3080, and the course of data processing by CY7C68013 and the data transfer by SPI bus and USB bus and completed the software design of data storage, analysis, display and network transmission in host computer.

  1. Circulating S100B and Adiponectin in Children Who Underwent Open Heart Surgery and Cardiopulmonary Bypass

    Directory of Open Access Journals (Sweden)

    Alessandro Varrica

    2015-01-01

    Full Text Available Background. S100B protein, previously proposed as a consolidated marker of brain damage in congenital heart disease (CHD newborns who underwent cardiac surgery and cardiopulmonary bypass (CPB, has been progressively abandoned due to S100B CNS extra-source such as adipose tissue. The present study investigated CHD newborns, if adipose tissue contributes significantly to S100B serum levels. Methods. We conducted a prospective study in 26 CHD infants, without preexisting neurological disorders, who underwent cardiac surgery and CPB in whom blood samples for S100B and adiponectin (ADN measurement were drawn at five perioperative time-points. Results. S100B showed a significant increase from hospital admission up to 24 h after procedure reaching its maximum peak (P0.05 have been found all along perioperative monitoring. ADN/S100B ratio pattern was identical to S100B alone with the higher peak at the end of CPB and remained higher up to 24 h from surgery. Conclusions. The present study provides evidence that, in CHD infants, S100B protein is not affected by an extra-source adipose tissue release as suggested by no changes in circulating ADN concentrations.

  2. 主动配电网背景下无功电压控制方法研究综述%Research Overview on Reactive Power Voltage Control Method Under Background of Active Distribution Network

    Institute of Scientific and Technical Information of China (English)

    倪鹏; 孙富荣

    2016-01-01

    介绍了主动配电网(ADN)背景下分布式电源(DG)接入对配电网电压分布和电压稳定性影响,从电网结构优化调压措施、电网设备的调压措施、电力系统无功优化调度调压措施等方面阐述了ADN无功电压控制方法。基于当前研究现状,总结了现有关于ADN无功电压控制研究存在的不足,并指出了在未来亟待解决的几个问题。%Introduction was made to the impacts of distributed generation (DG) access under background of active distribution network (ADN) on the voltage distribution of DG and voltage stability. This paper expounded on the ADN reactive power voltage control method from the aspects of pressure regulating measures of power network structure optimization, power network equipment, optimal reactive power dispatch of power system and so on. Based on the current research status, this paper summarized the shortcomings existing in the research on ADN reactive power voltage control and pointed out several problems which are much-needed to solve in the future.

  3. ADNK-6013有线激光游戏鼠标套件

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    ADNK-6013光学鼠标设计套件采用安捷伦ADNS-6010高性能激光浏览传感器和Cypress CY7C63743-PXCUSB微控制器。可满足厂商开发业内最高性能激光游戏鼠标的需求,加速产品上市的解决方案。ADNS-6010具有2000epi的最高分辨率、45ips的最大速度和7080fps的帧速率。它与ADNS-6120光学镜片、ADNS-6230固定夹和ADNV-6330激光二极管相结合,形成了一套完整的激光鼠标跟踪系统。CY7C63743-PXC微控制器和安捷伦ADNS-6010传感器之间的通信通过集成式串行外设接口完成。Cypress的EPROM微控制器可轻松地实现硬件的更改。

  4. Exploring the character of rural businesses

    DEFF Research Database (Denmark)

    Finke, Hanne Bat; Bosworth, Gary

    2016-01-01

    these assets can create value for ural businesses. Understanding these issues can better inform organisations that are seeking to support the rural economy adn rural communities.It can also guide buisness owners themselves as to how the may benefit from being a part of, or associated with, the rural economy...

  5. Laser Mouse Circuit Design Based on MEG16%基于MEG16的激光鼠标电路设计

    Institute of Scientific and Technical Information of China (English)

    刘孝赵; 吴振磊; 张锟

    2012-01-01

    提出一种以AVR单片机ATmegal6为核心、以nRF2401和ADNS-7050芯片为控制对象的无线激光鼠标设计.介绍了nRF2401和ADNS-7050的主要特性,设计了电压转换电路,解决了硬件电路设计问题.实验结果表明,该设计模块在运行过程中,性能可靠,工作稳定.%This paper points out a kind of wireless laser mouse design whose core is AVR monolithic ATmegal6 and whose control object is ADNS-7050 chip, describes the main characteristics of nRF2401 and ADNS-7050, designs a voltage conversion circuit which resolves the issue of hardware circuit design. Experimental results show that the design module is reliable and stable in running process.

  6. Tip loss correction for actuator / Navier Stokes computations

    DEFF Research Database (Denmark)

    Shen, Wen Zhong; Sørensen, Jens Nørkær; Mikkelsen, Robert Flemming

    2004-01-01

    The new tip loss correction, initially developed for ID BEM computations [1], is now extended to 2D Actuator Disc / Navier-Stokes (AD/NS) computations and 3D Actuator Line / Navier-Stokes (AL/NS) computations. As shown in the paper, the tip loss correction is an important and necessary step...

  7. Enhanced and sustained CD8+ T cell responses with an adenoviral vector-based hepatitis C virus vaccine encoding NS3 linked to the MHC class II chaperone protein invariant chain

    DEFF Research Database (Denmark)

    Mikkelsen, Marianne; Holst, Peter Johannes; Bukh, Jens

    2011-01-01

    memory. Functionally, the AdIiNS3-vaccinated mice had a significantly increased cytotoxic capacity compared with the AdNS3 group. The AdIiNS3-induced CD8(+) T cells protected mice from infection with recombinant vaccinia virus expressing HCV NS3 of heterologous 1b strains, and studies in knockout mice...

  8. Diversification at Financial Institutions and Systemic Crises

    NARCIS (Netherlands)

    Wagner, W.B.

    2006-01-01

    We show that the diversification of risks at financial institutions has unwelcome effects by increasing the likelihood of systems crises.As a result, complete diversification is not warranted adn the optimal degree of diversification is arbitrarily low.We also identify externalities that cause finan

  9. Environmental Analysis of Lake Pontchartrain, Louisiana, Its Surrounding Wetlands, and Selected Land Uses. Volume 2.

    Science.gov (United States)

    1980-02-01

    species. Proc. Nat. Acad. Sci. 43:293-295. Margalef, R. 1957. La teoria de la informacion en ecologia. Mem. Real. Acad. Ciencias y Artes de Barcelona. 32...Symphurus plagiusa Cynoscion arenarius Monacanthus hispidus Cynoscion nebulosus Sphoeroides parvus Leiostomus xanthurus 815 80 @a.C 4@ ON C z u 0j AdN r O

  10. Competency of Graduate Nurses as Perceived by Nurse Preceptors and Nurse Managers

    Science.gov (United States)

    Wise, Vanessa

    2013-01-01

    As newly graduated associate degree nurses (ADN) and baccalaureate degree nurses (BSN) enter into the workforce, they must be equipped to care for a complex patient population; therefore, the purpose of this study was to address the practice expectations and clinical competency of new nurses as perceived by nurse preceptors and nurse managers.…

  11. Disinhibition of neurons of the nucleus of solitary tract that project to the superior salivatory nucleus causes choroidal vasodilation: Implications for mechanisms underlying choroidal baroregulation.

    Science.gov (United States)

    Li, Chunyan; Fitzgerald, Malinda E C; Del Mar, Nobel; Reiner, Anton

    2016-10-28

    Preganglionic neurons in the superior salivatory nucleus (SSN) that mediate parasympathetic vasodilation of choroidal blood vessels receive a major excitatory input from the baroresponsive part of the nucleus of the solitary tract (NTS). This input appears likely to mediate choroidal vasodilation during systemic hypotension, which prevents decreases in choroidal blood flow (ChBF) due to reduced perfusion pressure. It is uncertain, however, how low blood pressure signals to NTS from the aortic depressor nerve (ADN), which fires at a low rate during systemic hypotension, could yield increased firing in the NTS output to SSN. The simplest hypothesis is that SSN-projecting NTS neurons are under the inhibitory control of ADN-receptive GABAergic NTS neurons. As part of evaluating this hypothesis, we assessed if SSN-projecting NTS neurons, in fact, receive prominent inhibitory input and if blocking GABAergic modulation of them increases ChBF. We found that SSN-projecting NTS neuronal perikarya identified by retrograde labeling are densely coated with GABAergic terminals, but lightly coated with excitatory terminals. We also found that, infusion of the GABA-A receptor antagonist GABAzine into NTS increased ChBF. Our results are consistent with the possibility that low blood pressure signals from the ADN produce vasodilation in choroid by causing diminished activity in ADN-receptive NTS neurons that tonically suppress SSN-projecting NTS neurons.

  12. Exploring the character of rural businesses: Performing change and continuity

    DEFF Research Database (Denmark)

    Finke, Hanne Bat; Bosworth, Gary

    2016-01-01

    these assets can create value for ural businesses. Understanding these issues can better inform organisations that are seeking to support the rural economy adn rural communities.It can also guide buisness owners themselves as to how the may benefit from being a part of, or associated with, the rural economy...

  13. Determinación de mutaciones del gen NRAS en pacientes con cáncer colorrectal

    OpenAIRE

    Pardo Llama, Mikel

    2014-01-01

    El objetivo de este trabajo es identificar las mutaciones del gen NRAS en el ADN procedente de muestras de tejido de pacientes con cáncer colorrectal para verificar su relación con el mismo y con la respuesta a los tratamientos para esta patología. Máster en Biología Molecular y Biomedicina

  14. A Cyber Physical Model Based on a Hybrid System for Flexible Load Control in an Active Distribution Network

    Directory of Open Access Journals (Sweden)

    Yun Wang

    2017-02-01

    Full Text Available To strengthen the integration of the primary and secondary systems, a concept of Cyber Physical Systems (CPS is introduced to construct a CPS in Power Systems (Power CPS. The most basic work of the Power CPS is to build an integration model which combines both a continuous process and a discrete process. The advanced form of smart grid, the Active Distribution Network (ADN is a typical example of Power CPS. After designing the Power CPS model architecture and its application in ADN, a Hybrid System based model and control method of Power CPS is proposed in this paper. As an application example, ADN flexible load is modeled and controlled with ADN feeder power control by a control strategy which includes the normal condition and the underpowered condition. In this model and strategy, some factors like load power consumption and load functional demand are considered and optimized. In order to make up some of the deficiencies of centralized control, a distributed control method is presented to reduce model complexity and improve calculation speed. The effectiveness of all the models and methods are demonstrated in the case study.

  15. Global Broadcast Service Reach Back Via Ultra High Frequency Demand Assigned Multiple Access Satellite Communications

    Science.gov (United States)

    1998-06-01

    program developed to implement the Navy’s Copernicus vision. A major portion of JMCOMS is the Automated Digital Network System (ADNS). The goal of...EC/ME Monterey, CA 93943 CAPT Joseph Delpino GBS/JPO care of DISA D216 C4&I Programs Directorate Skyline 5 5111 Leesburg Pike Falls Church

  16. Nursing Student Retention in Associate Degree Nursing Programs Utilizing a Retention Specialist

    Science.gov (United States)

    Schrum, Ronna A.

    2014-01-01

    The purpose of this study was to examine specific variables associated with nursing student retention in Associate Degree Nursing (ADN) Programs. Jeffreys (2004) Nursing Undergraduate Retention and Success (NURS) conceptual model provided the framework for this descriptive correlational study. One hundred sixty eight pre-licensure associate degree…

  17. Service-Oriented Architecture Afloat: A Capabilities-Based Prioritization Scheme

    Science.gov (United States)

    2013-04-01

    packet to arrive is the first to leave—or by Cisco Weighted Random Early Detection (WRED). WRED works by having the network router (ADNS in this...statistics for engineers and scientists (3rd ed.). Belmont, CA: Thomson Higher Education. Horton, M. C. (2012). Examination of a capabilities-based

  18. Relationship of Academic Variables to National Council for Licensure Examination for Registered Nurse Performance of Graduates in a Selected Associate Degree Nursing Program.

    Science.gov (United States)

    Naron, Rosarica G.; Widlak, Frederic

    This report addresses the unstable and unsatisfactory performance of Chicago, Illinois' Olive-Harvey College's (OHC) associate degree nursing (ADN) graduates on the National Council Licensure Examination for Registered Nurses (NCLEX-RN). An ex post facto correlation study was designed to determine the worthiness of pre-nursing admission course…

  19. Predictors for Associate Degree Nursing Students' First Attempt on NCLEX-RN

    Science.gov (United States)

    Smith, Barbara A.

    2011-01-01

    Nursing program administrators need to identify significant predictors for associate degree nursing (ADN) students to determine characteristics of those who will most likely pass the NCLEX-RN® on the first attempt. The purpose of the quantitative study with a correlation prediction design was to determine if a relationship existed between the…

  20. 10 años de la secuenciación del genoma humano: Encuentro entre el imaginario y la realidad

    Directory of Open Access Journals (Sweden)

    María Teresa Reguero Reza

    2013-06-01

    Full Text Available Lejano parece en año de 1953 cuando se publica en Nature un artículo sobre la estructura helicoidal de ADN, propuesta por dos investigadores, el británico Francis Crick y el estadounidense James Watson, lo que constituyó la piedra angular de los procesos de secuenciación.

  1. Utilization of stable isotopes for studying biological macromolecules by high-resolution NMR; Utilisation des isotopes stables pour l`etude de macromolecules biologiques par R.M.N. a haute resolution

    Energy Technology Data Exchange (ETDEWEB)

    Jamin, N.; Gilles, N.; Lirsac, P.N.; Menez, A.; Toma, F. [CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Direction des Sciences du Vivant; Bostadt, A.; Gabrielsen, O.S. [Oslo Univ. (Norway)

    1994-12-31

    Stable isotope labelling applications for nuclear magnetic resonance study of proteins are reviewed and several examples of utilization of these techniques are given. Resolution limitations for very large protein are discussed. Examples are also given for labelling other biomolecules such as ARN and ADN with {sup 2}H, {sup 15}N and {sup 13}C (3D structure of proteins). 2 figs., 39 refs.

  2. Predicting Success for Nontraditional Students in an Afternoon and Evening/Weekend Associate Degree in Nursing Program

    Science.gov (United States)

    Ledesma, Hernani Luison, Jr.

    2012-01-01

    Mount St. Mary's College has offered a nontraditional Associate Degree in Nursing (ADN) Program since 1992. The program has an afternoon and evening/weekend format. There has been one previous research study published in 2005 that described the student population that Mount St. Mary's College serves. This present study will examine the…

  3. Evaluation of quail and chicken embryos for the detection of botulinum toxin serotypes A, B, E and F activity.

    Science.gov (United States)

    Comparison of quail (Coturnix japonica) and chicken (Gallus domesticus) embryos for the detection of BoNT/A activity was conducted using equal dosages of toxin/g of embryo (quail at 7 g and chickens at 48 g). Quail embryos were injected at 0, 0.5 to 50 ng adn chicken embryos at 0, 3.4 to 342 ng and...

  4. A thymidineless Escherichia coli strain useful for in vivo DNA labeling

    Directory of Open Access Journals (Sweden)

    Viana Manrique Suárez

    2004-01-01

    Full Text Available Los métodos de detección no radiactivos eliminan las desventajas que representa el uso de la radioactividad en el diagnóstico médico. Tanto el ADN plasmídico como el cromosomal han sido marcados in vivo con el empleo de la 5-fluordesoxiuridina (FdUrd como inhibidor de la timidilato sintasa (mé- todo FdUrd. Con el objetivo de evitar el empleo de FdUrd fue obtenida una cepa de Escherichia coli auxótrofa para la timidina (thy-. Dicha cepa fue utilizada para el marcaje in vivo con BrdUrd de ADN plasmídico (método thy-. La cepa de E. coli, DH5a fue mutagenizada con N-metil-N'-nitro-N-nitrosoguanidina. Se seleccionó una colonia mediante crecimiento selectivo en medio mínimo y medio mínimo con suplemento de timidina (0,6 g/L. La cepa mostró un índice de reversión menor que 3,93 · 10-8 en medio mínimo con suplemento. Se realizó un estudio comparativo in vivo de estos sistemas de marcaje. La detección del ADN simple y doble cadena marcado con BrdUrd se llevó a cabo inmunoenzimáticamente. El valor límite de detección fue de 1 ng para el ADN simple cadena marcado por el método thy- y de 5 ng para el marcado por el método FdUrd. El valor de detección límite está relacionado con la cantidad de ADN, simple o doble cadena, disponible para la detección por anticuerpo anti-5- BrdUrd. La sensibilidad del sistema es comparable a la de otros sistemas no radioactivos de marcaje de ADN. Estos resultados demuestran la capacidad de la cepa thy- para el marcaje in vivo de ADN como un sistema capaz de producir grandes cantidades de sonda marcada.

  5. Kinetic analysis of overlapping multistep thermal decomposition comprising exothermic and endothermic processes: thermolysis of ammonium dinitramide.

    Science.gov (United States)

    Muravyev, Nikita V; Koga, Nobuyoshi; Meerov, Dmitry B; Pivkina, Alla N

    2017-01-25

    This study focused on kinetic modeling of a specific type of multistep heterogeneous reaction comprising exothermic and endothermic reaction steps, as exemplified by the practical kinetic analysis of the experimental kinetic curves for the thermal decomposition of molten ammonium dinitramide (ADN). It is known that the thermal decomposition of ADN occurs as a consecutive two step mass-loss process comprising the decomposition of ADN and subsequent evaporation/decomposition of in situ generated ammonium nitrate. These reaction steps provide exothermic and endothermic contributions, respectively, to the overall thermal effect. The overall reaction process was deconvoluted into two reaction steps using simultaneously recorded thermogravimetry and differential scanning calorimetry (TG-DSC) curves by considering the different physical meanings of the kinetic data derived from TG and DSC by P value analysis. The kinetic data thus separated into exothermic and endothermic reaction steps were kinetically characterized using kinetic computation methods including isoconversional method, combined kinetic analysis, and master plot method. The overall kinetic behavior was reproduced as the sum of the kinetic equations for each reaction step considering the contributions to the rate data derived from TG and DSC. During reproduction of the kinetic behavior, the kinetic parameters and contributions of each reaction step were optimized using kinetic deconvolution analysis. As a result, the thermal decomposition of ADN was successfully modeled as partially overlapping exothermic and endothermic reaction steps. The logic of the kinetic modeling was critically examined, and the practical usefulness of phenomenological modeling for the thermal decomposition of ADN was illustrated to demonstrate the validity of the methodology and its applicability to similar complex reaction processes.

  6. Spatial and temporal controls on Alnus-derived nutrients and stream stoichiometry: Implications for aquatic ecosystem productivity

    Science.gov (United States)

    Devotta, D.; Fraterrigo, J.; Walsh, P.; Hu, F.

    2015-12-01

    Predicting how nutrient fluxes that cross ecosystem boundaries will respond to future climate change is one of the greatest challenges for ecology in the 21st century. In southwestern (SW) Alaska, Pacific salmon (Oncorhynchus spp.) and nitrogen (N)-fixation by alder (Alnus spp.) provide key nutrient subsidies to freshwater systems. The importance of alder-derived nutrients (ADN) to aquatic systems will increase as alder cover expands under climate warming and salmon harvesting reduces marine-derived nutrients. We investigate broad-scale spatial and temporal drivers of ADN and stream N:P in 26 streams in SW Alaska. Alder cover and watershed features were measured using satellite images and topographic maps in ArcGIS. Stream water samples were collected in each spring and summer from 2010-2013 and analyzed for dissolved N and total phosphorus (TP). We obtained annual growing season length (AGSL) and sum of growing degree days (GDD) data from weather stations. Elevation was inversely related to alder cover, stream N, and N:P (ρ=-0.802, -0.65, and -0.71 resp., pADN export. Higher P was associated with lower temperatures, possibly reflecting reduced P demand under low rates of metabolic activity. Structural equation modeling revealed significant causal relationships among elevation, alder cover, and stream N:P across multiple years (r2=0.94, X2=742.8, df=9, pADN fluxes and stream N:P than temporal variation in growing season conditions. Therefore, the aquatic productivity of streams at low elevations that receive large amounts of ADN will be most resilient to climate change.

  7. EPIDEMIOLOGÍA MOLECULAR DE BARTONELLA HENSELAE EN GATOS CALLEJEROS Y DE ALBERGUE EN ZARAGOZA, ESPAÑA

    Directory of Open Access Journals (Sweden)

    Manuel Alamán Valtierra

    2016-01-01

    Full Text Available Bartonella henselae produce la enfermedad del araña- zo del gato en las personas y se considera infradiagnosticada. El objetivo fue detectar y cuantificar la carga de ácido desoxiribonucleico (ADN de B. henselae en muestras de sangre y orales de gatos callejeros y de albergue de Zaragoza, España y analizar su relación con factores epidemiológicos y clínicos. Métodos: Se estudiaron 47 gatos. El ADN de B. henselae ,se detectó mediante reacción en cadena de la polimerasa en tiempo real (qPCR en sangre y muestras orales. Se usó el paquete estadístico SPSS para analizar la positividad de las muestras pareadas y su relación con factores epidemioló- gicos (edad, sexo, origen, mes de muestreo, presencia de pulgas/garrapatas y clínicos (estado de salud y presencia de lesiones orales. Se realizó un análisis de regresión logística para conocer la asociación entre la presencia en sangre y cavidad oral y el resto de las variables. Resultados: el 23,40% de las muestras de sangre y el 27,65% de las orales portaba el ADN de B. henselae . Se observó débil correlación de la positividad de las muestras pareadas (kappa= 0,33; p 0,05 entre la presencia de ADN de B. henselae en las muestras y los factores epidemiológicos y clínicos. Los gatos con lesiones orales portaban una carga más elevada de ADN (3,12/1x10 6 células en la boca que los que no tenían lesiones (2,58 /1por10 6 células, (p=0,032. Conclusiones: La detección de ADN de B. henselae en sangre no pare- ce estar relacionada con su presencia en cavidad oral y viceversa. Los gatos positivos con lesiones orales pueden significar mayor riesgo de infección por B. henselae para las personas que los manejan.

  8. A Wolf Pack Algorithm for Active and Reactive Power Coordinated Optimization in Active Distribution Network

    Science.gov (United States)

    Zhuang, H. M.; Jiang, X. J.

    2016-08-01

    This paper presents an active and reactive power dynamic optimization model for active distribution network (ADN), whose control variables include the output of distributed generations (DGs), charge or discharge power of energy storage system (ESS) and reactive power from capacitor banks. To solve the high-dimension nonlinear optimization model, a new heuristic swarm intelligent method, namely wolf pack algorithm (WPA) with better global convergence and computational robustness, is adapted so that the network loss minimization can be achieved. In this paper, the IEEE33-bus system is used to show the effectiveness of WPA technique compared with other techniques. Numerical tests on the modified IEEE 33-bus system show that WPA for active and reactive multi-period optimization of ADN is exact and effective.

  9. El mapa como metáfora o la espacialización del pensamiento

    OpenAIRE

    Lois, Carla

    2015-01-01

    La expresión “mapa” se usa para nombrar imágenes no geográficas: mapa del ADN, “mapa de sitio” de navegabilidad online, “mapas conceptuales”, entre muchas otras. ¿Por qué (o en qué sentido) estas imágenes son mapas? Una posible respuesta a esa pregunta consiste en revisar la capacidad que tiene la imagen cartográfica para funcionar como metáfora de otras imágenes, ideas, pensamientos. A expressão “mapa” usa-se para nomear imagens não geográficas: o mapa do ADN, “mapa do site” na navegabili...

  10. HTPB/ADN推进剂反应气孔产生机理研究%Reaction Mechanism of Forming Pore in HTPB/AND Propellants

    Institute of Scientific and Technical Information of China (English)

    胥会祥; 庞维强; 李勇宏; 张楠楠; 王晓红

    2009-01-01

    为揭示HTPB/ADN/AP/Al推进剂产生气孔的原因,制备了一系列含ADN和TEA、T-313、MAPO、HX-752等键合剂的推进剂样品,试验确定与ADN反应产生气孔的组分,并通过DSC/TG-IR/MS联用仪分析了产生气孔的反应机理.结果表明,含固化剂TDI、IPDI和醇胺类键合剂TEA、T-313的推进剂样品不产生气孔,而含氮丙啶类键合剂MAPO、HX-752的样品固化后均出现气孔.DSC法证实MAPO与ADN产生强烈的作用,使ADN的主要放热分解峰温度降低99.7 ℃.在50 ℃,MAPO与ADN混合物(质量比1:1)加热2 h的过程形成了气体产物: N_2O、NO_2,并通过质谱检测到其存在.分析认为,推进剂中氮丙啶类键合剂促使了ADN的分解,形成反应气孔.%In order to reveal the reasons of forming pore in hydroxyl terminated polybutadiene/ammonium dinitramide/ammonium perchlorate/aluminium ( HTPB/ADN/AP/Al ) composite propellants, a series of propellant samples containing ADN and triethanolamine ( TEA ) , triethanolamine trifluoroboron complex ( T-313), tris ( 2-methylaziridinyl) phosphine oxide ( MAPO ) , isoph-thaloyl-bis-(2-methylaziridine) (HX-752) were prepared,and the components of reacting with ADN and forming pore were confirmed, and the reaction mechanism was analyzed by DSC/TG-IR/MS. Results show that there are no pores in the propellant samples containing curing agents such as toluene diisocyanate(TDI) ,isophrone diisocyanate(IPDI) and ethanolamine bonding agents such as TEA, T-313 respectively, but the pores are formed in the samples containing aziridine bonding agents MAPO and HX-752. It is proved by DSC that there is a strong interaction between ADN and MAPO,which decreases the temperature of the main decomposition peak of ADN by about 99.7 ℃ . When the mixture of ADN and MAPO ( mass ratio 1:1) was heated continuously at 50 ℃ for 2 h ,the gasous products N_2O and NO_2 are farmed and detected by mass spectrum. It is considered that the aziridine bonding agents can accelerate

  11. 光电鼠标芯片组在无接触检测运动物体中的应用

    Institute of Scientific and Technical Information of China (English)

    林邓伟; 邢文生

    2006-01-01

    安捷伦公司的ADNS-2051芯片广泛应用在光电鼠标中,是一种高性能的运动检测器件。本文介绍了一种基于ADNS-2051芯片组、单片机等器件组成的系统,能够在无接触条件下检测运动物体的方向和速度,具有灵敏度高、抗干扰性强等特点。文章重点介绍系统的硬件与软件设计。

  12. Situation awareness of active distribution network: roadmap, technologies, and bottlenecks

    DEFF Research Database (Denmark)

    Lin, Jin; Wan, Can; Song, Yonghua

    2016-01-01

    With the rapid development of local generation and demand response, the active distribution network (ADN), which aggregates and manages miscellaneous distributed resources, has moved from theory to practice. Secure and optimal operations now require an advanced situation awareness (SA) system so...... in the project of developing an SA system as the basic component of a practical active distribution management system (ADMS) deployed in Beijing, China, is presented. This paper reviews the ADN’s development roadmap by illustrating the changes that are made in elements, topology, structure, and control scheme....... Taking into consideration these hardware changes, a systematic framework is proposed for the main components and the functional hierarchy of an SA system for the ADN. The SA system’s implementation bottlenecks are also presented, including, but not limited to issues in big data platform, distribution...

  13. Imuno-expressão da DNMT1, DNMT3a e DNMT3b nos tumores odontogênicos

    OpenAIRE

    Leonardo Borges Ferro

    2013-01-01

    Os tumores odontogênicos são um grupo heterogéneo de lesões formadas a partir de tecidos que dão origem ao dente. A metilação do ADN, uma adição covalente de um grupo metilo na posição 5 de carbono de um nucleótideo de citosina, é considerado um importante regulador da expressão génica. A adição do radical metil é catalisada por ADN metiltransferases (DNMTs). Embora alguns estudos epigenéticos tenham sido realizados em tumores odontogênicos, um estudo com os três tipos de DNMTs em vários memb...

  14. Advanced neuroprotection for brain ischemia: an alternative approach to minimize stroke damage.

    Science.gov (United States)

    Ayuso, Maria Irene; Montaner, Joan

    2015-01-01

    Despite decades of research on neuroprotectants in the fight against ischemic stroke, no successful results have been obtained and new alternative approaches are urgently needed. Translation of effective candidate drugs in experimental studies to patients has systematically failed. However, some of those treatments or neuroprotectant diets which demonstrated only beneficial effects if given before (but not after) ischemia induction and discarded for conventional neuroprotection, could be rescued in order to apply an 'advanced neuroprotection strategy' (ADNES). Herein, the authors discuss how re-profiling those neuroprotective candidate drugs and diets with the best potential, some of which are mentioned in this article as an ADNES, may be a good approach for developing successful treatments that protect the brain against ischemic damage. This novel approach would try to protect the brain of patients who are at high risk of suffering a stroke, before damage occurs, in order to minimize brain injury by having the neuroprotectant drug or diet 'on board' if unfortunately stroke occurs.

  15. Une expérience scientifique et didactique : enseigner l’informatique aux biologistes

    OpenAIRE

    2014-01-01

    Tout a commencé avec James D. Watson, Francis Crick, Rosalind Elsie Franklin et quelques autres dans les années 1950. Ils nous ont appris que la conformation des êtres vivants se déduisait de l’information génétique codée par l’acide désoxyribonucléique (ADN) de leurs cellules. Les macromolécules (on dit aussi polymères) d’ADN sont de longues chaînes de motifs moléculaires élémentaires, les nucléotides, dont il existe quatre variétés : l’adénine (notée A), la thymine (notée T), la cytosine (n...

  16. Oscillatory synchrony between head direction cells recorded bilaterally in the anterodorsal thalamic nuclei.

    Science.gov (United States)

    Butler, William N; Taube, Jeffrey Steven

    2017-03-01

    The head direction (HD) circuit is a complex, interconnected network of brain regions ranging from the brainstem to the cortex. Recent work found that HD cells co-recorded ipsilaterally in the anterodorsal nucleus (ADN) of the thalamus displayed coordinated firing patterns. A high frequency oscillation pattern (130-160 Hz) was visible in the cross-correlograms of these HD cell pairs. Spectral analysis further found that the power of this oscillation was greatest at 0 ms and decreased at greater lags, and demonstrated that there was greater synchrony between HD cells with similar tunings. Here, we demonstrate that the same high frequency synchrony exists in HD cell pairs recorded contralaterally from one another in the bilateral ADN. When we examined the cross-correlograms of HD cells that were co-recorded bilaterally we observed the same high frequency (~150-200 Hz) oscillatory relationship. The strength of this synchrony was similar to the synchrony seen in ipsilateral HD cell pairs, and the degree of synchrony in each cross-correlogram was dependent on the difference in tuning between the two cells. Additionally, the frequency rate of this oscillation appeared to be independent of the firing rates of the two cross-correlated cells. Taken together, these results imply that the left and right thalamic HD network are functionally related, despite an absence of direct anatomical projections. However, anatomical tracing has found that each of the lateral mammillary nuclei (LMN) project bilaterally to both of the ADN, suggesting the LMN may be responsible for the functional connectivity observed between the two ADN.

  17. High pressure structural, elastic and vibrational properties of green energetic oxidizer ammonium dinitramide

    Science.gov (United States)

    Yedukondalu, N.; Ghule, Vikas D.; Vaitheeswaran, G.

    2016-08-01

    Ammonium DiNitramide (ADN) is one of the most promising green energetic oxidizers for future rocket propellant formulations. In the present work, we report a detailed theoretical study on structural, elastic, and vibrational properties of the emerging oxidizer under hydrostatic compression using various dispersion correction methods to capture weak intermolecular (van der Waals and hydrogen bonding) interactions. The calculated ground state lattice parameters, axial compressibilities, and equation of state are in good accord with the available experimental results. Strength of intermolecular interactions has been correlated using the calculated compressibility curves and elastic moduli. Apart from this, we also observe discontinuities in the structural parameters and elastic constants as a function of pressure. Pictorial representation and quantification of intermolecular interactions are described by the 3D Hirshfeld surfaces and 2D finger print maps. In addition, the computed infra-red (IR) spectra at ambient pressure reveal that ADN is found to have more hygroscopic nature over Ammonium Perchlorate (AP) due to the presence of strong hydrogen bonding. Pressure dependent IR spectra show blue- and red-shift of bending and stretching frequencies which leads to weakening and strengthening of the hydrogen bonding below and above 5 GPa, respectively. The abrupt changes in the calculated structural, mechanical, and IR spectra suggest that ADN might undergo a first order structural transformation to a high pressure phase around 5-6 GPa. From the predicted detonation properties, ADN is found to have high and low performance characteristics (DCJ = 8.09 km/s and PCJ = 25.54 GPa) when compared with ammonium based energetic oxidizers (DCJ = 6.50 km/s and PCJ = 17.64 GPa for AP, DCJ = 7.28 km/s and PCJ = 18.71 GPa for ammonium nitrate) and well-known secondary explosives for which DCJ = ˜8-10 km/s and PCJ = ˜30-50 GPa, respectively.

  18. OBJETIVOS DE LA INSPECCIÓN EDUCATIVA DE ANDALUCÍA PARA EL CUATRIENIO 2012/2016. UNA INVITACIÓN AL DEBATE

    Directory of Open Access Journals (Sweden)

    Francisco Javier Fernández Franco

    2013-12-01

    La inspección educativa en Andalucía, centra sus esfuerzos en un modelo homologado de intervención, centrado en los centros y con presencia en las aulas (allí donde se produce el impacto directo del proceso de enseñanza y aprendizaje, donde se fragua el meollo y el ADN de todo sistema educativo para mejorar los procesos, los resultados y la organización y funcionamiento de los centros.

  19. High flash point electrolyte for use in lithium-ion batteries

    Energy Technology Data Exchange (ETDEWEB)

    Isken, P.; Dippel, C.; Schmitz, R.; Schmitz, R.W.; Kunze, M.; Passerini, S.; Winter, M. [Institute of Physical Chemistry, Westfaelische Wilhelms-University Muenster, Corrensstrasse 28/30, 48149 Muenster (Germany); Lex-Balducci, A., E-mail: a.lex-balducci@uni-muenster.de [Institute of Physical Chemistry, Westfaelische Wilhelms-University Muenster, Corrensstrasse 28/30, 48149 Muenster (Germany)

    2011-09-01

    Highlights: > Substitution of linear carbonates in conventional electrolytes with adiponitrile allows the realization of high flash point electrolytes. > EC:ADN based electrolytes display a higher anodic stability than a conventional electrolyte based on EC:DEC. > Graphite and NCM electrodes used in combination with the EC:ADN based electrolyte display a performance comparable with that of conventional electrolytes. - Abstract: The high flash point solvent adiponitrile (ADN) was investigated as co-solvent with ethylene carbonate (EC) for use as lithium-ion battery electrolyte. The flash point of this solvent mixture was more than 110 deg. C higher than that of conventional electrolyte solutions involving volatile linear carbonate components, such as diethyl carbonate (DEC) or dimethyl carbonate (DMC). The electrolyte based on EC:ADN (1:1 wt) with lithium tetrafluoroborate (LiBF{sub 4}) displayed a conductivity of 2.6 mS cm{sup -1} and no aluminum corrosion. In addition, it showed higher anodic stability on a Pt electrode than the standard electrolyte 1 M lithium hexafluorophosphate (LiPF{sub 6}) in EC:DEC (3:7 wt). Graphite/Li half cells using this electrolyte showed excellent rate capability up to 5C and good cycling stability (more than 98% capacity retention after 50 cycles at 1C). Additionally, the electrolyte was investigated in NCM/Li half cells. The cells were able to reach a capacity of 104 mAh g{sup -1} at 5C and capacity retention of more than 97% after 50 cycles. These results show that an electrolyte with a considerably increased flash point with respect to common electrolyte systems comprising linear carbonates, could be realized without any negative effects on the electrochemical performance in Li-half cells.

  20. 二硝酰胺铵防吸湿技术研究进展%Research Progress in Anti-Hygroscopicity of Ammonium Dinitramide

    Institute of Scientific and Technical Information of China (English)

    刘波; 刘少武; 于慧芳; 魏伦; 郑双; 王琼林; 韩冰

    2011-01-01

    二硝酰胺铵(ADN)的吸湿性是影响其广泛应用的一个主要原因.从ADN的吸湿原因、吸湿机理、防止吸湿方法几方面介绍了ADN防吸湿技术的研究进展,并指出了我国今后ADN防吸湿技术的发展方向.

  1. DNA Vaccination of the American Crow (Corvus brachyrhynchos) Provides Partial Protection Against Lethal Challenge with West Nile Virus

    Science.gov (United States)

    2007-01-01

    protección parcial contra el desafı́o letal con el virus del Oeste del Nilo. La cepa del virus del Oeste del Nilo aislada en Nueva York en el año 1999 es...murieron. La administración parenteral de la vacuna de ADN del virus del Oeste del Nilo estuvo asociada con una reducción de la mortalidad pero no

  2. Six Feet Under o la muerte nuestra de cada día

    Directory of Open Access Journals (Sweden)

    Francisco Guillermo Medina Montaño

    2015-12-01

    Full Text Available A partir del modelo de análisis ADN de las series televisivas, los autores abordan la serie Six Feet Under, su estructura narrativa, su lenguaje audiovisual, así como la construcción psicológica y moral de los personajes que en ella convergen para así escudriñar los mensajes y valores de una de las obras más vistas en su tipo y en su tiempo.

  3. Method for the replication, amplification or sequencing of DNA template.

    OpenAIRE

    2009-01-01

    [ES] La presente invención se encuadra dentro del campo de la biotecnología. Específicamente, se refiere a un método para llevar a cabo la replicación, la amplificación o la secuenciación de un ácido desoxirribonucleico con una ADN polimerasa del tipo cp29 y a un kit para llevar a cabo dicho método.

  4. Adsorptive desulfurization and denitrogenation using metal-organic frameworks.

    Science.gov (United States)

    Ahmed, Imteaz; Jhung, Sung Hwa

    2016-01-15

    With the increasing worldwide demand for energy, utilization of fossil fuels is increasing proportionally. Additionally, new and unconventional energy sources are also being utilized at an increasing rate day-by-day. These sources, along with some industrial processes, result in the exposal of several sulfur- and nitrogen-containing compounds (SCCs and NCCs, respectively) to the environment, and the exposure is one of the greatest environmental threats in the recent years. Although, several methods were established for the removal of these pollutants during the last few decades, recent advancements in adsorptive desulfurization and denitrogenation (ADS and ADN, respectively) with metal-organic frameworks (MOFs) make this the most promising and remarkable method. Therefore, many research groups are currently involved with ADS and ADN with MOFs, and the results are improving gradually by modifying the MOF adsorbents according to several specific adsorption mechanisms. In this review, ADS and ADN studies are thoroughly discussed for both liquid-phase and gas-phase adsorption. The MOF modification procedures, which are important for improved adsorption, are also described. To improve the knowledge among the scientific community, it is very important to understand the detailed chemistry and mechanism involved in a chemical process, which also creates the possibility and pathway for further developments in research and applications. Therefore, the mechanisms related to the adsorption procedures are also discussed in detail. From this review, it can be expected that the scientific community will obtain an understanding of the current state of ADS and ADN, their importance, and some encouragement and insight to take the research knowledge base to a higher level.

  5. Deciphering the transcriptional-regulatory network of flocculation in Schizosaccharomyces pombe.

    Science.gov (United States)

    Kwon, Eun-Joo Gina; Laderoute, Amy; Chatfield-Reed, Kate; Vachon, Lianne; Karagiannis, Jim; Chua, Gordon

    2012-01-01

    In the fission yeast Schizosaccharomyces pombe, the transcriptional-regulatory network that governs flocculation remains poorly understood. Here, we systematically screened an array of transcription factor deletion and overexpression strains for flocculation and performed microarray expression profiling and ChIP-chip analysis to identify the flocculin target genes. We identified five transcription factors that displayed novel roles in the activation or inhibition of flocculation (Rfl1, Adn2, Adn3, Sre2, and Yox1), in addition to the previously-known Mbx2, Cbf11, and Cbf12 regulators. Overexpression of mbx2(+) and deletion of rfl1(+) resulted in strong flocculation and transcriptional upregulation of gsf2(+)/pfl1(+) and several other putative flocculin genes (pfl2(+)-pfl9(+)). Overexpression of the pfl(+) genes singly was sufficient to trigger flocculation, and enhanced flocculation was observed in several combinations of double pfl(+) overexpression. Among the pfl1(+) genes, only loss of gsf2(+) abrogated the flocculent phenotype of all the transcription factor mutants and prevented flocculation when cells were grown in inducing medium containing glycerol and ethanol as the carbon source, thereby indicating that Gsf2 is the dominant flocculin. In contrast, the mild flocculation of adn2(+) or adn3(+) overexpression was likely mediated by the transcriptional activation of cell wall-remodeling genes including gas2(+), psu1(+), and SPAC4H3.03c. We also discovered that Mbx2 and Cbf12 displayed transcriptional autoregulation, and Rfl1 repressed gsf2(+) expression in an inhibitory feed-forward loop involving mbx2(+). These results reveal that flocculation in S. pombe is regulated by a complex network of multiple transcription factors and target genes encoding flocculins and cell wall-remodeling enzymes. Moreover, comparisons between the flocculation transcriptional-regulatory networks of Saccharomyces cerevisiae and S. pombe indicate substantial rewiring of transcription

  6. New World Vistas: Air and Space Power for the 21st Century, Materials Volume.

    Science.gov (United States)

    1996-06-01

    cyclodextrine nitrate, and HTREL, plus new oxidizers such as ADN that are currently available, and the developing area of high nitrogen compounds that have... encapsulation , bondline venting of off-gases, microthin injectors, microcombustion chambers, and solar absorbing heat exchangers. Figure 12.3 shows a...structures through molecular encapsulation need to be investigated, and this is where nanophased polymers of the nanoparticle type may play an

  7. Overcoming DNA extraction problems from carnivorous plants

    Directory of Open Access Journals (Sweden)

    Fleischmann, Andreas

    2009-12-01

    Full Text Available We tested previously published protocols for DNA isolation from plants with high contents of polyphenols and polysaccharides for several taxa of carnivorous plants. However, we did not get satisfying results with fresh or silica dried leaf tissue obtained from field collected or greenhouse grown plants, nor from herbarium specimens. Therefore, we have developed a simple modified protocol of the commercially available Macherey- Nagel NucleoSpin® Plant kit for rapid, effective and reproducible isolation of high quality genomic DNA suitable for PCR reactions. DNA extraction can be conducted from both fresh and dried leaf tissue of various carnivorous plant taxa, irrespective of high contents of polysaccharides, phenolic compounds and other secondary plant metabolites that interfere with DNA isolation and amplification.

    Probamos algunos protocolos publicados previamente para el aislamiento del ADN de plantas con alto contenido de polifenoles y polisacáridos para varios táxones de plantas carnívoras. Sin embargo, no conseguimos muy buenos resultados ni con tejidos de hojas frescas, ni con tejidos de hojas secadas en gel de sílice obtenidas de plantas colectadas en el campo o cultivadas en los invernaderos, ni de especímenes de herbario. Por lo tanto, hemos desarrollado un protocolo sencillo, modificado del Macherey- Nagel NucleoSpin® Plant kit disponible en el mercado para el aislamiento rápido, eficaz y reproducible de ADN genómico de alta calidad conveniente para la reacción en cadena de la polimerasa. La extracción del ADN se puede realizar en tejidos de hojas frescas o secas de varios táxones de plantas carnívoras, sin importar el grado de contenido de polisacáridos, compuestos fenólicos u otros metabolitos secundarios que interfieren con el aislamiento y la amplificación del ADN.

  8. Adenovirus Vectors Expressing Hantavirus Proteins Protect Hamsters against Lethal Challenge with Andes Virus ▿

    OpenAIRE

    2009-01-01

    Hantaviruses infect humans following aerosolization from rodent feces and urine, producing hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. Due to the high rates of mortality and lack of therapies, vaccines are urgently needed. Nonreplicating adenovirus (Ad) vectors that express Andes hantavirus (ANDV) nucleocapsid protein (AdN) or glycoproteins (AdGN and AdGC) were constructed. Ad vectors were tested for their ability to protect Syrian hamsters from a lethal ANDV infe...

  9. Ahmar, otro hijo de Iblis (el diablo) en el Miscel??neo de Salom??n

    OpenAIRE

    1997-01-01

    Ahmar Ibn Iblis aparece con sus cinco hermanos en el Hadit de Salom??n, relato recogido en el libro manuscrito Miscel??neo de Salom??n. Ahmar es un ser infernal que da??a a `los hijos de Ad??n' produci??ndoles enfermedades mentales. Salom??n le obliga con amenazas a que le cuente la sintomatolog??a de la enfermedad y su remedio. Ahmar obedece y cuenta todo.

  10. Analysis list: ADNP2 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ADNP2 Digestive tract + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/ADN...P2.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/ADNP2.5.tsv http://dbarchive.bioscienced...bc.jp/kyushu-u/hg19/target/ADNP2.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/ADNP2.Digestive

  11. Normalización de un sistema de reacción en cadena de la polimerasa en tiempo real para la cuantificación de papilomavirus humano de alto riesgo oncogénico

    Directory of Open Access Journals (Sweden)

    Yudira Soto

    2012-04-01

    Full Text Available El objetivo de este trabajo fue normalizar e implementar un sistema de reacción en cadena de la polimerasa en tiempo real, para determinar la carga viral de 7 genotipos de papilomavirus humano (PVH de alto riesgo oncogénico. Se evaluó la especificidad del sistema y se construyeron las curvas estándar para PVH 16 y 18, que se emplearon para la cuantificación de ADN viral en diferentes muestras de pacientes identificados como positivos a PVH, mediante reacción en cadena de la polimerasa (RCP cualitativa y secuenciación nucleotídica. Se obtuvieron dos curvas estándar para PVH 16 y 18, a partir del ADN genómico de las líneas celulares SiHa y HeLa, las que mostraron una buena correlación lineal ( r = -0,99 y valores bajos de error. El límite inferior de detección a partir del ADN de las líneas celulares fue de hasta 10 copias para ambos genotipos. No se obtuvo reacción cruzada entre los diferentes tipos de PVH ni con otros virus ADN. La reacción en cadena de la polimerasa en tiempo real (RCP-TR normalizada probó ser un sistema simple, rápido, específico y altamente sensible. Además, permitirá desarrollar investigaciones sobre la prevalencia de infección por PVH en Cuba, con vistas a la aplicación de las vacunas que se encuentran disponibles en el mercado internacional, así como la evaluación de otros candidatos vacunales diseñados en el futuro.

  12. Synthesis and Characterization of FOX-12%N-脒基脲二硝酰胺盐(FOX-12)的合成与表征

    Institute of Scientific and Technical Information of China (English)

    杨通辉; 何金选; 张海林

    2004-01-01

    以二硝酰胺铵(ADN)和双氰胺为原材料经过一系列反应,制备出新型有机二硝酰胺盐-N-脒基脲二硝酰胺盐(FOX-12),并鉴定了其结构,测定了其熔点、感度、吸湿性等性能.

  13. United States Arms Transfers as a Consistent Element of United States Foreign Policy

    Science.gov (United States)

    1978-09-01

    friendly countries, 55 4. To control ,:xports, whether government-to-govern- ment or on a comercial basis, 5. To ensure sales were consistent with and... Politica in a Revolu~.onqry hago 2d adN’Yorkt T. B, ipfTnc6 t Uompany, 19(il. Lird; Melvin R. Statement of th#_ S3qro t.Iry. ofD~os Molvin it. ai~r be)TiaJin

  14. Rethinking National Security

    Science.gov (United States)

    1990-10-01

    1989. (U 162 .H29 1989) Hanrieder. Wolfram F., ed. Global Peace and Security: Trend# and Challone . Boulder: Westvlew, 1987. (JX 1952 .G0538 1987...41. Haley. P. Iduard. and Jack Merritt. Nuclear Itratefl, &M Cotl. sA hs Lalut. Boulder: Uestview, 19868. (U 263 .1765 1988) Halloran. Bernard F.. ed...press, Stanford University, 1987. (UA 23 .W38934 1987) Weinberg, Alvin Martin, and Jack N. Barkenbus. Stratojlc Defenses adn AM Con New York: Paragon

  15. Human Information Processing of Targets and Real-World Scenes.

    Science.gov (United States)

    1985-07-30

    dichotomous (or trichotomous) contrasts (e.g., voiced vs unvoiced, nasal vs oral ) on a handful of attributes, one need not make particularly fine...have componential descriptions that differ considerably from one exemplar to another, as with a pole lamp vs a ginger jar table lamp, for example. The...Hildebrandt, K. A., & Fitzgerald, H. E. (1983). The infant’& physical attractiveness: Its effect on bonding adn attachment. Infant Rental Health Journal

  16. Microfluidic Power Generation

    Science.gov (United States)

    2008-03-28

    electricity and magnetism we of ancient time, neither one had any practical application at the time and were simply observed as curiosities [24]. The first...mechanical energy to magnetic energy. The water flowing through the microchannels will induce mechanical pressure on the turbine, causing it to rotate...C. Panchal, and L. Genens, "Design and Cost of Near-term OTEC Plants for the Production of Desalinated Water adn Electric Power," U. S. D. o. Energy

  17. Marcadores moleculares y ecología del movimiento

    Directory of Open Access Journals (Sweden)

    J.J. Robledo-Arnuncio

    2009-01-01

    Full Text Available El estudio del movimiento es parte esencial del análisis ecológico a nivel de gen, individuo, población, comunidad y ecosistema. Los marcadores de ADN son herramientas muy útiles para el estudio del movimiento a todos estos niveles, permitiendo, por ejemplo, establecer relaciones de parentesco entre individuos, determinar el origen poblacional de individuos migrantes, estudiar sistemas de apareamiento, cuantificar la escala de interacciones interespecíficas, o caracterizar el patrón espacial de la dispersión y la competencia. A nivel de gen, los marcadores de ADN son imprescindibles para estudiar la estructura y el flujo genético dentro y entre poblaciones, así como su efecto combinado con otras fuerzas evolutivas y los factores bióticos y abióticos del medio sobre la adaptación local. En este artículo se revisan algunos métodos basados en marcadores de ADN que pueden resultar de utilidad en ecología del movimiento, identificándose áreas de estudio en las que se espera estos marcadores sean de especial utilidad en los próximos años.

  18. Time course of the hemodynamic responses to aortic depressor nerve stimulation in conscious spontaneously hypertensive rats

    Energy Technology Data Exchange (ETDEWEB)

    Durand, M.T.; Mota, A.L. [Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil); Barale, A.R. [Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG (Brazil); Castania, J.A.; Fazan, R. Jr.; Salgado, H.C. [Departamento de Fisiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP (Brazil)

    2012-03-16

    The time to reach the maximum response of arterial pressure, heart rate and vascular resistance (hindquarter and mesenteric) was measured in conscious male spontaneously hypertensive (SHR) and normotensive control rats (NCR; Wistar; 18-22 weeks) subjected to electrical stimulation of the aortic depressor nerve (ADN). The parameters of stimulation were 1 mA intensity and 2 ms pulse length applied for 5 s, using frequencies of 10, 30, and 90 Hz. The time to reach the hemodynamic responses at different frequencies of ADN stimulation was similar for SHR (N = 15) and NCR (N = 14); hypotension = NCR (4194 ± 336 to 3695 ± 463 ms) vs SHR (3475 ± 354 to 4494 ± 300 ms); bradycardia = NCR (1618 ± 152 to 1358 ± 185 ms) vs SHR (1911 ± 323 to 1852 ± 431 ms), and the fall in hindquarter vascular resistance = NCR (6054 ± 486 to 6550 ± 847 ms) vs SHR (4849 ± 918 to 4926 ± 646 ms); mesenteric = NCR (5574 ± 790 to 5752 ± 539 ms) vs SHR (5638 ± 648 to 6777 ± 624 ms). In addition, ADN stimulation produced baroreflex responses characterized by a faster cardiac effect followed by a vascular effect, which together contributed to the decrease in arterial pressure. Therefore, the results indicate that there is no alteration in the conduction of the electrical impulse after the site of baroreceptor mechanical transduction in the baroreflex pathway (central and/or efferent) in conscious SHR compared to NCR.

  19. Estandarización de una prueba de PCR para la detección de Brucella sp.

    Directory of Open Access Journals (Sweden)

    Carlos Padilla R

    2003-04-01

    Full Text Available Objetivo: Estandarizar una prueba de PCR para la detección de Brucella spp. Materiales y métodos: Se usó oligonucleótidos reportados que amplifican la secuencia de 16S rRNA de Brucella spp. Fueron evaluados dos métodos de extracción de ADN: fenol-cloroformo-alcohol isoamílico y un kit comercial basado en columnas con afinidad. Para determinar la sensibilidad de la prueba se usó 8 cepas peruanas de Brucella y para determinar la especificidad de la prueba se usó otras cepas bacterianas peruanas de E. coli, Shigella, Proteus mirabilis, Salmonella aratyphi, Salmonella typhi, Citrobacter freundii y Vibrio cholerae. Resultados: Los 2 métodos de extracción de ADN evaluados fueron efectivos. La sensibilidad analítica de la prueba es alta, lográndose detectar 80 femtogramos de ADN de Brucella spp. purificado. Todas las cepas peruanas de Brucella spp. fueron detectadas por la prueba. Además, la prueba es negativa para cepas peruanas de otras especies bacterianas. Conclusión: Se ha estandarizado las condiciones de una prueba de PCR para la detección de cepas peruanas de Brucella spp., la cual es muy sensible y específica en el laboratorio.

  20. Diseño y estandarización de una prueba de PCR para el diagnóstico de la Bartonelosis causada por Bartonella bacilliformis

    Directory of Open Access Journals (Sweden)

    Carlos Padilla R

    2003-03-01

    Full Text Available Objetivo: Diseñar una prueba de PCR para el diagnóstico de la Bartonelosis causada por Bartonella bacilliformis. Materiales y métodos: Se usó la secuencia del locus de invasión ialB para diseñar los oligonucleótidos ialBF y ialBR, además del ADN geonómico purificado de una cepa referencial de B. bacilliformis para estandarizar las condiciones de la prueba. Finalmente, la prueba fue preliminarmente evaluada con 12 cepas de B. bacilliormis aisladas en 3 áreas endémicas y 10 muestras de sangre total de pacientes con Bartonelosis confirmada. Resultados: La prueba detectó el ADN de aislamientos de B. bacilliformis de 3 áreas bartonelósicas endémicas del Perú: Ancash, Cuzco y Lima; mientras que no detectó el ADN de B. hensenlae, ni de B. vinsonii, ni de otras bacterias y parásitos. Además, esta prueba fue positiva para 10 muestras sanguíneas de pacientes con bartonelosis confirmada y negativa para 5 muestras de pacientes con malaria por P. falciparum. Conclusión: Esta prueba de PCR podría ser útil para el diagnóstico de la bartonelosis causada por B. bacilliformis.