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Sample records for adaptive optics scanning

  1. Reflective afocal broadband adaptive optics scanning ophthalmoscope

    Science.gov (United States)

    Dubra, Alfredo; Sulai, Yusufu

    2011-01-01

    A broadband adaptive optics scanning ophthalmoscope (BAOSO) consisting of four afocal telescopes, formed by pairs of off-axis spherical mirrors in a non-planar arrangement, is presented. The non-planar folding of the telescopes is used to simultaneously reduce pupil and image plane astigmatism. The former improves the adaptive optics performance by reducing the root-mean-square (RMS) of the wavefront and the beam wandering due to optical scanning. The latter provides diffraction limited performance over a 3 diopter (D) vergence range. This vergence range allows for the use of any broadband light source(s) in the 450-850 nm wavelength range to simultaneously image any combination of retinal layers. Imaging modalities that could benefit from such a large vergence range are optical coherence tomography (OCT), multi- and hyper-spectral imaging, single- and multi-photon fluorescence. The benefits of the non-planar telescopes in the BAOSO are illustrated by resolving the human foveal photoreceptor mosaic in reflectance using two different superluminescent diodes with 680 and 796 nm peak wavelengths, reaching the eye with a vergence of 0.76 D relative to each other. PMID:21698035

  2. Adaptive optics scanning ophthalmoscopy with annular pupils

    Science.gov (United States)

    Sulai, Yusufu N.; Dubra, Alfredo

    2012-01-01

    Annular apodization of the illumination and/or imaging pupils of an adaptive optics scanning light ophthalmoscope (AOSLO) for improving transverse resolution was evaluated using three different normalized inner radii (0.26, 0.39 and 0.52). In vivo imaging of the human photoreceptor mosaic at 0.5 and 10° from fixation indicates that the use of an annular illumination pupil and a circular imaging pupil provides the most benefit of all configurations when using a one Airy disk diameter pinhole, in agreement with the paraxial confocal microscopy theory. Annular illumination pupils with 0.26 and 0.39 normalized inner radii performed best in terms of the narrowing of the autocorrelation central lobe (between 7 and 12%), and the increase in manual and automated photoreceptor counts (8 to 20% more cones and 11 to 29% more rods). It was observed that the use of annular pupils with large inner radii can result in multi-modal cone photoreceptor intensity profiles. The effect of the annular masks on the average photoreceptor intensity is consistent with the Stiles-Crawford effect (SCE). This indicates that combinations of images of the same photoreceptors with different apodization configurations and/or annular masks can be used to distinguish cones from rods, even when the former have complex multi-modal intensity profiles. In addition to narrowing the point spread function transversally, the use of annular apodizing masks also elongates it axially, a fact that can be used for extending the depth of focus of techniques such as adaptive optics optical coherence tomography (AOOCT). Finally, the positive results from this work suggest that annular pupil apodization could be used in refractive or catadioptric adaptive optics ophthalmoscopes to mitigate undesired back-reflections. PMID:22808435

  3. Adaptive optics scanning ophthalmoscopy with annular pupils.

    Science.gov (United States)

    Sulai, Yusufu N; Dubra, Alfredo

    2012-07-01

    Annular apodization of the illumination and/or imaging pupils of an adaptive optics scanning light ophthalmoscope (AOSLO) for improving transverse resolution was evaluated using three different normalized inner radii (0.26, 0.39 and 0.52). In vivo imaging of the human photoreceptor mosaic at 0.5 and 10° from fixation indicates that the use of an annular illumination pupil and a circular imaging pupil provides the most benefit of all configurations when using a one Airy disk diameter pinhole, in agreement with the paraxial confocal microscopy theory. Annular illumination pupils with 0.26 and 0.39 normalized inner radii performed best in terms of the narrowing of the autocorrelation central lobe (between 7 and 12%), and the increase in manual and automated photoreceptor counts (8 to 20% more cones and 11 to 29% more rods). It was observed that the use of annular pupils with large inner radii can result in multi-modal cone photoreceptor intensity profiles. The effect of the annular masks on the average photoreceptor intensity is consistent with the Stiles-Crawford effect (SCE). This indicates that combinations of images of the same photoreceptors with different apodization configurations and/or annular masks can be used to distinguish cones from rods, even when the former have complex multi-modal intensity profiles. In addition to narrowing the point spread function transversally, the use of annular apodizing masks also elongates it axially, a fact that can be used for extending the depth of focus of techniques such as adaptive optics optical coherence tomography (AOOCT). Finally, the positive results from this work suggest that annular pupil apodization could be used in refractive or catadioptric adaptive optics ophthalmoscopes to mitigate undesired back-reflections.

  4. Adaptive optics scanning laser ophthalmoscope imaging: technology update

    Directory of Open Access Journals (Sweden)

    Merino D

    2016-04-01

    Full Text Available David Merino, Pablo Loza-Alvarez The Institute of Photonic Sciences (ICFO, The Barcelona Institute of Science and Technology, Castelldefels, Barcelona, Spain Abstract: Adaptive optics (AO retinal imaging has become very popular in the past few years, especially within the ophthalmic research community. Several different retinal techniques, such as fundus imaging cameras or optical coherence tomography systems, have been coupled with AO in order to produce impressive images showing individual cell mosaics over different layers of the in vivo human retina. The combination of AO with scanning laser ophthalmoscopy has been extensively used to generate impressive images of the human retina with unprecedented resolution, showing individual photoreceptor cells, retinal pigment epithelium cells, as well as microscopic capillary vessels, or the nerve fiber layer. Over the past few years, the technique has evolved to develop several different applications not only in the clinic but also in different animal models, thanks to technological developments in the field. These developments have specific applications to different fields of investigation, which are not limited to the study of retinal diseases but also to the understanding of the retinal function and vision science. This review is an attempt to summarize these developments in an understandable and brief manner in order to guide the reader into the possibilities that AO scanning laser ophthalmoscopy offers, as well as its limitations, which should be taken into account when planning on using it. Keywords: high-resolution, in vivo retinal imaging, AOSLO

  5. MULTIMODAL IMAGING OF ACUTE EXUDATIVE POLYMORPHOUS VITELLIFORM MACULOPATHY WITH OPTICAL COHERENCE TOMOGRAPHY ANGIOGRAPHY AND ADAPTIVE OPTICS SCANNING LASER OPHTHALMOSCOPY.

    Science.gov (United States)

    Skondra, Dimitra; Nesper, Peter L; Fawzi, Amani A

    2017-05-16

    To report a case of acute exudative polymorphous vitelliform maculopathy including the findings of optical coherence tomography angiography and adaptive optics scanning laser ophthalmoscopy. Findings on clinical examination, color fundus photography, spectral-domain optical coherence tomography, infrared reflectance, autofluorescence, optical coherence tomography angiography, and adaptive optics scanning laser ophthalmoscopy. A 54-year-old white man with no significant medical history and history of smoking presented with bilateral multiple serous and vitelliform detachments consistent with acute exudative polymorphous vitelliform maculopathy. Extensive infectious, inflammatory, and malignancy workup was negative. Spectral-domain optical coherence tomography showed thickened, hyperreflective ellipsoid zone, subretinal fluid, and focal as well as diffuse subretinal hyperreflective material corresponding to the vitelliform lesions. Optical coherence tomography angiography showed normal retinal and choroidal vasculature, whereas adaptive optics scanning laser ophthalmoscopy showed circular focal "target" lesions at the level of the photoreceptors in the area of foveal detachment. Multimodal imaging is valuable in evaluating patients with acute exudative polymorphous vitelliform maculopathy.

  6. Adaptive optics scanning laser ophthalmoscopy in fundus imaging, a review and update.

    Science.gov (United States)

    Zhang, Bing; Li, Ni; Kang, Jie; He, Yi; Chen, Xiao-Ming

    2017-01-01

    Adaptive optics scanning laser ophthalmoscopy (AO-SLO) has been a promising technique in funds imaging with growing popularity. This review firstly gives a brief history of adaptive optics (AO) and AO-SLO. Then it compares AO-SLO with conventional imaging methods (fundus fluorescein angiography, fundus autofluorescence, indocyanine green angiography and optical coherence tomography) and other AO techniques (adaptive optics flood-illumination ophthalmoscopy and adaptive optics optical coherence tomography). Furthermore, an update of current research situation in AO-SLO is made based on different fundus structures as photoreceptors (cones and rods), fundus vessels, retinal pigment epithelium layer, retinal nerve fiber layer, ganglion cell layer and lamina cribrosa. Finally, this review indicates possible research directions of AO-SLO in future.

  7. Adaptive optics scanning laser ophthalmoscopy in fundus imaging, a review and update

    Directory of Open Access Journals (Sweden)

    Bing Zhang

    2017-11-01

    Full Text Available Adaptive optics scanning laser ophthalmoscopy (AO-SLO has been a promising technique in funds imaging with growing popularity. This review firstly gives a brief history of adaptive optics (AO and AO-SLO. Then it compares AO-SLO with conventional imaging methods (fundus fluorescein angiography, fundus autofluorescence, indocyanine green angiography and optical coherence tomography and other AO techniques (adaptive optics flood-illumination ophthalmoscopy and adaptive optics optical coherence tomography. Furthermore, an update of current research situation in AO-SLO is made based on different fundus structures as photoreceptors (cones and rods, fundus vessels, retinal pigment epithelium layer, retinal nerve fiber layer, ganglion cell layer and lamina cribrosa. Finally, this review indicates possible research directions of AO-SLO in future.

  8. Cone and Rod Loss in Stargardt Disease Revealed by Adaptive Optics Scanning Light Ophthalmoscopy

    Science.gov (United States)

    Song, Hongxin; Rossi, Ethan A.; Latchney, Lisa; Bessette, Angela; Stone, Edwin; Hunter, Jennifer J.; Williams, David R.; Chung, Mina

    2015-01-01

    Importance Stargardt disease (STGD1) is characterized by macular atrophy and flecks in the retinal pigment epithelium. The causative ABCA4 gene encodes a protein localizing to photoreceptor outer segments. The pathologic steps by which ABCA4 mutations lead to clinically detectable retinal pigment epithelium changes remain unclear. We investigated early STGD1 using adaptive optics scanning light ophthalmoscopy. Observations Adaptive optics scanning light ophthalmoscopy imaging of 2 brothers with early STGD1 and their unaffected parents was compared with conventional imaging. Cone and rod spacing were increased in both patients (P optics scanning light ophthalmoscopy reveals increased cone and rod spacing in areas that appear normal in conventional images, suggesting that photoreceptor loss precedes clinically detectable retinal pigment epithelial disease in STGD1. PMID:26247787

  9. Active eye-tracking for an adaptive optics scanning laser ophthalmoscope.

    Science.gov (United States)

    Sheehy, Christy K; Tiruveedhula, Pavan; Sabesan, Ramkumar; Roorda, Austin

    2015-07-01

    We demonstrate a system that combines a tracking scanning laser ophthalmoscope (TSLO) and an adaptive optics scanning laser ophthalmoscope (AOSLO) system resulting in both optical (hardware) and digital (software) eye-tracking capabilities. The hybrid system employs the TSLO for active eye-tracking at a rate up to 960 Hz for real-time stabilization of the AOSLO system. AOSLO videos with active eye-tracking signals showed, at most, an amplitude of motion of 0.20 arcminutes for horizontal motion and 0.14 arcminutes for vertical motion. Subsequent real-time digital stabilization limited residual motion to an average of only 0.06 arcminutes (a 95% reduction). By correcting for high amplitude, low frequency drifts of the eye, the active TSLO eye-tracking system enabled the AOSLO system to capture high-resolution retinal images over a larger range of motion than previously possible with just the AOSLO imaging system alone.

  10. Non-common path aberration correction in an adaptive optics scanning ophthalmoscope.

    Science.gov (United States)

    Sulai, Yusufu N; Dubra, Alfredo

    2014-09-01

    The correction of non-common path aberrations (NCPAs) between the imaging and wavefront sensing channel in a confocal scanning adaptive optics ophthalmoscope is demonstrated. NCPA correction is achieved by maximizing an image sharpness metric while the confocal detection aperture is temporarily removed, effectively minimizing the monochromatic aberrations in the illumination path of the imaging channel. Comparison of NCPA estimated using zonal and modal orthogonal wavefront corrector bases provided wavefronts that differ by ~λ/20 in root-mean-squared (~λ/30 standard deviation). Sequential insertion of a cylindrical lens in the illumination and light collection paths of the imaging channel was used to compare image resolution after changing the wavefront correction to maximize image sharpness and intensity metrics. Finally, the NCPA correction was incorporated into the closed-loop adaptive optics control by biasing the wavefront sensor signals without reducing its bandwidth.

  11. Detection of airbag impact-induced cone photoreceptor damage by adaptive optics scanning laser ophthalmoscopy: a case report

    National Research Council Canada - National Science Library

    Kaizu, Yoshihiro; Nakao, Shintaro; Yamaguchi, Muneo; Murakami, Yusuke; Salehi-Had, Hani; Ishibashi, Tatsuro

    2016-01-01

    The purpose of this study was to report a case of traumatic maculopathy with para-central visual field defects following an impact by airbag deployment using adaptive optics scanning laser ophthalmoscopy (AO-SLO...

  12. High-speed adaptive optics line scan confocal retinal imaging for human eye.

    Science.gov (United States)

    Lu, Jing; Gu, Boyu; Wang, Xiaolin; Zhang, Yuhua

    2017-01-01

    Continuous and rapid eye movement causes significant intraframe distortion in adaptive optics high resolution retinal imaging. To minimize this artifact, we developed a high speed adaptive optics line scan confocal retinal imaging system. A high speed line camera was employed to acquire retinal image and custom adaptive optics was developed to compensate the wave aberration of the human eye's optics. The spatial resolution and signal to noise ratio were assessed in model eye and in living human eye. The improvement of imaging fidelity was estimated by reduction of intra-frame distortion of retinal images acquired in the living human eyes with frame rates at 30 frames/second (FPS), 100 FPS, and 200 FPS. The device produced retinal image with cellular level resolution at 200 FPS with a digitization of 512×512 pixels/frame in the living human eye. Cone photoreceptors in the central fovea and rod photoreceptors near the fovea were resolved in three human subjects in normal chorioretinal health. Compared with retinal images acquired at 30 FPS, the intra-frame distortion in images taken at 200 FPS was reduced by 50.9% to 79.7%. We demonstrated the feasibility of acquiring high resolution retinal images in the living human eye at a speed that minimizes retinal motion artifact. This device may facilitate research involving subjects with nystagmus or unsteady fixation due to central vision loss.

  13. High-resolution adaptive optics scanning laser ophthalmoscope with multiple deformable mirrors

    Science.gov (United States)

    Chen, Diana C.; Olivier, Scot S.; Jones; Steven M.

    2010-02-23

    An adaptive optics scanning laser ophthalmoscopes is introduced to produce non-invasive views of the human retina. The use of dual deformable mirrors improved the dynamic range for correction of the wavefront aberrations compared with the use of the MEMS mirror alone, and improved the quality of the wavefront correction compared with the use of the bimorph mirror alone. The large-stroke bimorph deformable mirror improved the capability for axial sectioning with the confocal imaging system by providing an easier way to move the focus axially through different layers of the retina.

  14. Noninvasive imaging of the human rod photoreceptor mosaic using a confocal adaptive optics scanning ophthalmoscope

    Science.gov (United States)

    Dubra, Alfredo; Sulai, Yusufu; Norris, Jennifer L.; Cooper, Robert F.; Dubis, Adam M.; Williams, David R.; Carroll, Joseph

    2011-01-01

    The rod photoreceptors are implicated in a number of devastating retinal diseases. However, routine imaging of these cells has remained elusive, even with the advent of adaptive optics imaging. Here, we present the first in vivo images of the contiguous rod photoreceptor mosaic in nine healthy human subjects. The images were collected with three different confocal adaptive optics scanning ophthalmoscopes at two different institutions, using 680 and 775 nm superluminescent diodes for illumination. Estimates of photoreceptor density and rod:cone ratios in the 5°–15° retinal eccentricity range are consistent with histological findings, confirming our ability to resolve the rod mosaic by averaging multiple registered images, without the need for additional image processing. In one subject, we were able to identify the emergence of the first rods at approximately 190 μm from the foveal center, in agreement with previous histological studies. The rod and cone photoreceptor mosaics appear in focus at different retinal depths, with the rod mosaic best focus (i.e., brightest and sharpest) being at least 10 μm shallower than the cones at retinal eccentricities larger than 8°. This study represents an important step in bringing high-resolution imaging to bear on the study of rod disorders. PMID:21750765

  15. Multi-wavelength imaging with the adaptive optics scanning laser Ophthalmoscope

    Science.gov (United States)

    Grieve, Kate; Tiruveedhula, Pavan; Zhang, Yuhua; Roorda, Austin

    2006-12-01

    The adaptive optics scanning laser ophthalmoscope has been fitted with three light sources of different wavelengths to allow simultaneous or separate imaging with one, two or three wavelength combinations. The source wavelengths used are 532 nm, 658 nm and 840 nm. Typically the instrument is used in dual-frame mode, performing imaging at 840 nm and precisely coincident retinal stimulation in one of the visible wavelengths. Instrument set-up and single-detector image capture are described. Simultaneous multi-wavelength imaging in the living human retina is demonstrated. The chromatic aberrations of the human eye lead to lateral and axial shifts, as well as magnification differences in the image, from one wavelength to another. Measurement of these chromatic effects is described for instrument characterization purposes.

  16. ASSOCIATIONS BETWEEN MACULAR EDEMA AND CIRCULATORY STATUS IN EYES WITH RETINAL VEIN OCCLUSION: An Adaptive Optics Scanning Laser Ophthalmoscopy Study.

    Science.gov (United States)

    Iida, Yuto; Muraoka, Yuki; Uji, Akihito; Ooto, Sotaro; Murakami, Tomoaki; Suzuma, Kiyoshi; Tsujikawa, Akitaka; Arichika, Shigeta; Takahashi, Ayako; Miwa, Yuko; Yoshimura, Nagahisa

    2017-10-01

    To investigate associations between parafoveal microcirculatory status and foveal pathomorphology in eyes with macular edema (ME) secondary to retinal vein occlusion (RVO). Ten consecutive patients (10 eyes) with acute retinal vein occlusion were enrolled, 9 eyes of which received intravitreal ranibizumab (IVR) injections. Foveal morphologic changes were examined via optical coherence tomography (OCT), and parafoveal circulatory status was assessed via adaptive optics scanning laser ophthalmoscopy (AO-SLO). The mean parafoveal aggregated erythrocyte velocity (AEV) measured by adaptive optics scanning laser ophthalmoscopy in eyes with retinal vein occlusion was 0.99 ± 0.43 mm/second at baseline, which was significantly lower than that of age-matched healthy subjects (1.41 ± 0.28 mm/second, P = 0.042). The longitudinal adaptive optics scanning laser ophthalmoscopy examinations of each patient showed that parafoveal AEV was strongly inversely correlated with optical coherence tomography-measured central foveal thickness (CFT) over the entire observation period. Using parafoveal AEV and central foveal thickness measurements obtained at the first and second examinations, we investigated associations between differences in parafoveal AEV and central foveal thickness, which were significantly and highly correlated (r = -0.84, P = 0.002). Using adaptive optics scanning laser ophthalmoscopy in eyes with retinal vein occlusion macular edema, we could quantitatively evaluate the parafoveal AEV. A reduction or an increase in parafoveal AEV may be a clinical marker for the resolution or development/progression of macular edema respectively.

  17. Design of a Compact, Bimorph Deformable Mirror-Based Adaptive Optics Scanning Laser Ophthalmoscope.

    Science.gov (United States)

    He, Yi; Deng, Guohua; Wei, Ling; Li, Xiqi; Yang, Jinsheng; Shi, Guohua; Zhang, Yudong

    2016-01-01

    We have designed, constructed and tested an adaptive optics scanning laser ophthalmoscope (AOSLO) using a bimorph mirror. The simulated AOSLO system achieves diffraction-limited criterion through all the raster scanning fields (6.4 mm pupil, 3° × 3° on pupil). The bimorph mirror-based AOSLO corrected ocular aberrations in model eyes to less than 0.1 μm RMS wavefront error with a closed-loop bandwidth of a few Hz. Facilitated with a bimorph mirror at a stroke of ±15 μm with 35 elements and an aperture of 20 mm, the new AOSLO system has a size only half that of the first-generation AOSLO system. The significant increase in stroke allows for large ocular aberrations such as defocus in the range of ±600° and astigmatism in the range of ±200°, thereby fully exploiting the AO correcting capabilities for diseased human eyes in the future.

  18. High-Resolution Adaptive Optics Scanning Laser Ophthalmoscope with Dual Deformable Mirrors

    Energy Technology Data Exchange (ETDEWEB)

    Chen, D C; Jones, S M; Silva, D A; Olivier, S S

    2006-08-11

    Adaptive optics scanning laser ophthalmoscope (AO SLO) has demonstrated superior optical quality of non-invasive view of the living retina, but with limited capability of aberration compensation. In this paper, we demonstrate that the use of dual deformable mirrors can effectively compensate large aberrations in the human retina. We used a bimorph mirror to correct large-stroke, low-order aberrations and a MEMS mirror to correct low-stroke, high-order aberration. The measured ocular RMS wavefront error of a test subject was 240 nm without AO compensation. We were able to reduce the RMS wavefront error to 90 nm in clinical settings using one deformable mirror for the phase compensation and further reduced the wavefront error to 48 nm using two deformable mirrors. Compared with that of a single-deformable-mirror SLO system, dual AO SLO offers much improved dynamic range and better correction of the wavefront aberrations. The use of large-stroke deformable mirrors provided the system with the capability of axial sectioning different layers of the retina. We have achieved diffraction-limited in-vivo retinal images of targeted retinal layers such as photoreceptor layer, blood vessel layer and nerve fiber layers with the combined phase compensation of the two deformable mirrors in the AO SLO.

  19. Cone structure imaged with adaptive optics scanning laser ophthalmoscopy in eyes with nonneovascular age-related macular degeneration.

    Science.gov (United States)

    Zayit-Soudry, Shiri; Duncan, Jacque L; Syed, Reema; Menghini, Moreno; Roorda, Austin J

    2013-11-15

    To evaluate cone spacing using adaptive optics scanning laser ophthalmoscopy (AOSLO) in eyes with nonneovascular AMD, and to correlate progression of AOSLO-derived cone measures with standard measures of macular structure. Adaptive optics scanning laser ophthalmoscopy images were obtained over 12 to 21 months from seven patients with AMD including four eyes with geographic atrophy (GA) and four eyes with drusen. Adaptive optics scanning laser ophthalmoscopy images were overlaid with color, infrared, and autofluorescence fundus photographs and spectral domain optical coherence tomography (SD-OCT) images to allow direct correlation of cone parameters with macular structure. Cone spacing was measured for each visit in selected regions including areas over drusen (n = 29), at GA margins (n = 14), and regions without drusen or GA (n = 13) and compared with normal, age-similar values. Adaptive optics scanning laser ophthalmoscopy imaging revealed continuous cone mosaics up to the GA edge and overlying drusen, although reduced cone reflectivity often resulted in hyporeflective AOSLO signals at these locations. Baseline cone spacing measures were normal in 13/13 unaffected regions, 26/28 drusen regions, and 12/14 GA margin regions. Although standard clinical measures showed progression of GA in all study eyes, cone spacing remained within normal ranges in most drusen regions and all GA margin regions. Adaptive optics scanning laser ophthalmoscopy provides adequate resolution for quantitative measurement of cone spacing at the margin of GA and over drusen in eyes with AMD. Although cone spacing was often normal at baseline and remained normal over time, these regions showed focal areas of decreased cone reflectivity. These findings may provide insight into the pathophysiology of AMD progression. (ClinicalTrials.gov number, NCT00254605).

  20. High-resolution adaptive optics scanning laser ophthalmoscope with dual deformable mirrors for large aberration correction

    Energy Technology Data Exchange (ETDEWEB)

    Chen, D; Jones, S M; Silva, D A; Olivier, S S

    2007-01-25

    Scanning laser ophthalmoscopes with adaptive optics (AOSLO) have been shown previously to provide a noninvasive, cellular-scale view of the living human retina. However, the clinical utility of these systems has been limited by the available deformable mirror technology. In this paper, we demonstrate that the use of dual deformable mirrors can effectively compensate large aberrations in the human retina, making the AOSLO system a viable, non-invasive, high-resolution imaging tool for clinical diagnostics. We used a bimorph deformable mirror to correct low-order aberrations with relatively large amplitudes. The bimorph mirror is manufactured by Aoptix, Inc. with 37 elements and 18 {micro}m stroke in a 10 mm aperture. We used a MEMS deformable mirror to correct high-order aberrations with lower amplitudes. The MEMS mirror is manufactured by Boston Micromachine, Inc with 144 elements and 1.5 {micro}m stroke in a 3 mm aperture. We have achieved near diffraction-limited retina images using the dual deformable mirrors to correct large aberrations up to {+-} 3D of defocus and {+-} 3D of cylindrical aberrations with test subjects. This increases the range of spectacle corrections by the AO systems by a factor of 10, which is crucial for use in the clinical environment. This ability for large phase compensation can eliminate accurate refractive error fitting for the patients, which greatly improves the system ease of use and efficiency in the clinical environment.

  1. Photoreceptor perturbation around subretinal drusenoid deposits as revealed by adaptive optics scanning laser ophthalmoscopy.

    Science.gov (United States)

    Zhang, Yuhua; Wang, Xiaolin; Rivero, Ernesto Blanco; Clark, Mark E; Witherspoon, Clark Douglas; Spaide, Richard F; Girkin, Christopher A; Owsley, Cynthia; Curcio, Christine A

    2014-09-01

    To describe the microscopic structure of photoreceptors impacted by subretinal drusenoid deposits, also called pseudodrusen, an extracellular lesion associated with age-related macular degeneration (AMD), using adaptive optics scanning laser ophthalmoscopy (AOSLO). Observational case series. We recruited 53 patients with AMD and 10 age-similar subjects who had normal retinal health. All subjects underwent color fundus photography, infrared reflectance, red-free reflectance, autofluorescence, and spectral-domain optical coherence tomography (OCT). Subretinal drusenoid deposits were classified by a 3-stage OCT-based grading system. Lesions and surrounding photoreceptors were examined by AOSLO. Subretinal drusenoid deposits were found in 26 eyes of 13 patients with AMD and imaged by AOSLO and spectral-domain OCT in 18 eyes (n = 342 lesions). Spectral-domain OCT showed subretinal drusenoid deposits as highly reflective material accumulated internal to the retinal pigment epithelium. AOSLO revealed that photoreceptor reflectivity was qualitatively reduced by stage 1 subretinal drusenoid deposits and was greatly reduced by stage 2. AOSLO presented a distinct structure in stage 3, a hyporeflective annulus consisting of deflected, degenerated or absent photoreceptors. A central core with a reflectivity superficially resembling photoreceptors is formed by the lesion material itself. A hyporeflective gap in the photoreceptor ellipsoid zone on either side of this core shown in spectral-domain OCT corresponded to the hyporeflective annulus seen by AOSLO. AOSLO and multimodal imaging of subretinal drusenoid deposits indicate solid, space-filling lesions in the subretinal space. Associated retinal reflectivity changes are related to lesion stages and are consistent with perturbations to photoreceptors, as suggested by histology. Copyright © 2014 Elsevier Inc. All rights reserved.

  2. Increasing the field of view of adaptive optics scanning laser ophthalmoscopy.

    Science.gov (United States)

    Laslandes, Marie; Salas, Matthias; Hitzenberger, Christoph K; Pircher, Michael

    2017-11-01

    An adaptive optics scanning laser ophthalmoscope (AO-SLO) set-up with two deformable mirrors (DM) is presented. It allows high resolution imaging of the retina on a 4°×4° field of view (FoV), considering a 7 mm pupil diameter at the entrance of the eye. Imaging on such a FoV, which is larger compared to classical AO-SLO instruments, is allowed by the use of the two DMs. The first DM is located in a plane that is conjugated to the pupil of the eye and corrects for aberrations that are constant in the FoV. The second DM is conjugated to a plane that is located ∼0.7 mm anterior to the retina. This DM corrects for anisoplanatism effects within the FoV. The control of the DMs is performed by combining the classical AO technique, using a Shack-Hartmann wave-front sensor, and sensorless AO, which uses a criterion characterizing the image quality. The retinas of four healthy volunteers were imaged in-vivo with the developed instrument. In order to assess the performance of the set-up and to demonstrate the benefits of the 2 DM configuration, the acquired images were compared with images taken in conventional conditions, on a smaller FoV and with only one DM. Moreover, an image of a larger patch of the retina was obtained by stitching of 9 images acquired with a 4°×4° FoV, resulting in a total FoV of 10°×10°. Finally, different retinal layers were imaged by shifting the focal plane.

  3. The Scanning Optical Microscope.

    Science.gov (United States)

    Sheppard, C. J. R.

    1978-01-01

    Describes the principle of the scanning optical microscope and explains its advantages over the conventional microscope in the improvement of resolution and contrast, as well as the possibility of producing a picture from optical harmonies generated within the specimen.

  4. High-resolution imaging of the retinal nerve fiber layer in normal eyes using adaptive optics scanning laser ophthalmoscopy.

    Directory of Open Access Journals (Sweden)

    Kohei Takayama

    Full Text Available PURPOSE: To conduct high-resolution imaging of the retinal nerve fiber layer (RNFL in normal eyes using adaptive optics scanning laser ophthalmoscopy (AO-SLO. METHODS: AO-SLO images were obtained in 20 normal eyes at multiple locations in the posterior polar area and a circular path with a 3-4-mm diameter around the optic disc. For each eye, images focused on the RNFL were recorded and a montage of AO-SLO images was created. RESULTS: AO-SLO images for all eyes showed many hyperreflective bundles in the RNFL. Hyperreflective bundles above or below the fovea were seen in an arch from the temporal periphery on either side of a horizontal dividing line to the optic disc. The dark lines among the hyperreflective bundles were narrower around the optic disc compared with those in the temporal raphe. The hyperreflective bundles corresponded with the direction of the striations on SLO red-free images. The resolution and contrast of the bundles were much higher in AO-SLO images than in red-free fundus photography or SLO red-free images. The mean hyperreflective bundle width around the optic disc had a double-humped shape; the bundles at the temporal and nasal sides of the optic disc were narrower than those above and below the optic disc (P<0.001. RNFL thickness obtained by optical coherence tomography correlated with the hyperreflective bundle widths on AO-SLO (P<0.001 CONCLUSIONS: AO-SLO revealed hyperreflective bundles and dark lines in the RNFL, believed to be retinal nerve fiber bundles and Müller cell septa. The widths of the nerve fiber bundles appear to be proportional to the RNFL thickness at equivalent distances from the optic disc.

  5. Retinal damage in chloroquine maculopathy, revealed by high resolution imaging: a case report utilizing adaptive optics scanning laser ophthalmoscopy.

    Science.gov (United States)

    Bae, Eun Jin; Kim, Kyoung Rae; Tsang, Stephen H; Park, Sung Pyo; Chang, Stanley

    2014-02-01

    A 53-year-old Asian woman was treated with hydroxychloroquine and chloroquine for lupus erythematosus. Within a few years, she noticed circle-shaped shadows in her central vision. Upon examination, the patient's visual acuity was 20 / 25 in both eyes. Humphrey visual field (HVF) testing revealed a central visual defect, and fundoscopy showed a ring-shaped area of parafoveal retinal pigment epithelium depigmentation. Fundus autofluorescence imaging showed a hypofluorescent lesion consistent with bull's eye retinopathy. Adaptive optics scanning laser ophthalmoscope (AO-SLO) revealed patch cone mosaic lesions, in which cones were missing or lost. In addition, the remaining cones consisted of asymmetrical shapes and sizes that varied in brightness. Unlike previous studies employing deformable mirrors for wavefront aberration correction, our AO-SLO approach utilized dual liquid crystal on silicon spatial light modulators. Thus, by using AO-SLO, we were able to create a photographic montage consisting of high quality images. Disrupted cone AO-SLO images were matched with visual field test results and functional deficits were associated with a precise location on the montage, which allowed correlation of histological findings with functional changes determined by HVF. We also investigated whether adaptive optics imaging was more sensitive to anatomical changes compared with spectral-domain optical coherence tomography.

  6. Computational fluid dynamics assisted characterization of parafoveal hemodynamics in normal and diabetic eyes using adaptive optics scanning laser ophthalmoscopy.

    Science.gov (United States)

    Lu, Yang; Bernabeu, Miguel O; Lammer, Jan; Cai, Charles C; Jones, Martin L; Franco, Claudio A; Aiello, Lloyd Paul; Sun, Jennifer K

    2016-12-01

    Diabetic retinopathy (DR) is the leading cause of visual loss in working-age adults worldwide. Previous studies have found hemodynamic changes in the diabetic eyes, which precede clinically evident pathological alterations of the retinal microvasculature. There is a pressing need for new methods to allow greater understanding of these early hemodynamic changes that occur in DR. In this study, we propose a noninvasive method for the assessment of hemodynamics around the fovea (a region of the eye of paramount importance for vision). The proposed methodology combines adaptive optics scanning laser ophthalmoscopy and computational fluid dynamics modeling. We compare results obtained with this technique with in vivo measurements of blood flow based on blood cell aggregation tracking. Our results suggest that parafoveal hemodynamics, such as capillary velocity, wall shear stress, and capillary perfusion pressure can be noninvasively and reliably characterized with this method in both healthy and diabetic retinopathy patients.

  7. Image quality improvement in adaptive optics scanning laser ophthalmoscopy assisted capillary visualization using B-spline-based elastic image registration.

    Science.gov (United States)

    Uji, Akihito; Ooto, Sotaro; Hangai, Masanori; Arichika, Shigeta; Yoshimura, Nagahisa

    2013-01-01

    To investigate the effect of B-spline-based elastic image registration on adaptive optics scanning laser ophthalmoscopy (AO-SLO)-assisted capillary visualization. AO-SLO videos were acquired from parafoveal areas in the eyes of healthy subjects and patients with various diseases. After nonlinear image registration, the image quality of capillary images constructed from AO-SLO videos using motion contrast enhancement was compared before and after B-spline-based elastic (nonlinear) image registration performed using ImageJ. For objective comparison of image quality, contrast-to-noise ratios (CNRS) for vessel images were calculated. For subjective comparison, experienced ophthalmologists ranked images on a 5-point scale. All AO-SLO videos were successfully stabilized by elastic image registration. CNR was significantly higher in capillary images stabilized by elastic image registration than in those stabilized without registration. The average ratio of CNR in images with elastic image registration to CNR in images without elastic image registration was 2.10 ± 1.73, with no significant difference in the ratio between patients and healthy subjects. Improvement of image quality was also supported by expert comparison. Use of B-spline-based elastic image registration in AO-SLO-assisted capillary visualization was effective for enhancing image quality both objectively and subjectively.

  8. Adaptive optics scanning laser ophthalmoscope using liquid crystal on silicon spatial light modulator: Performance study with involuntary eye movement

    Science.gov (United States)

    Huang, Hongxin; Toyoda, Haruyoshi; Inoue, Takashi

    2017-09-01

    The performance of an adaptive optics scanning laser ophthalmoscope (AO-SLO) using a liquid crystal on silicon spatial light modulator and Shack-Hartmann wavefront sensor was investigated. The system achieved high-resolution and high-contrast images of human retinas by dynamic compensation for the aberrations in the eyes. Retinal structures such as photoreceptor cells, blood vessels, and nerve fiber bundles, as well as blood flow, could be observed in vivo. We also investigated involuntary eye movements and ascertained microsaccades and drifts using both the retinal images and the aberrations recorded simultaneously. Furthermore, we measured the interframe displacement of retinal images and found that during eye drift, the displacement has a linear relationship with the residual low-order aberration. The estimated duration and cumulative displacement of the drift were within the ranges estimated by a video tracking technique. The AO-SLO would not only be used for the early detection of eye diseases, but would also offer a new approach for involuntary eye movement research.

  9. Detection of airbag impact-induced cone photoreceptor damage by adaptive optics scanning laser ophthalmoscopy: a case report.

    Science.gov (United States)

    Kaizu, Yoshihiro; Nakao, Shintaro; Yamaguchi, Muneo; Murakami, Yusuke; Salehi-Had, Hani; Ishibashi, Tatsuro

    2016-07-08

    The purpose of this study was to report a case of traumatic maculopathy with para-central visual field defects following an impact by airbag deployment using adaptive optics scanning laser ophthalmoscopy (AO-SLO). A 51-year-old man was involved in a motor vehicular accident and his left eye was struck by the deployed airbag, resulting in a para-central scotoma. The patient underwent a full ophthalmologic examination, spectral-domain optical coherence tomography (SD-OCT), and imaging with prototype AO-SLO systems (Canon Inc.) at 14 and 22 months after the injury. Images focused on the photoreceptor layer were recorded in the foveal area, and a montage of AO-SLO images was created. On AO-SLO, focal dark areas could be observed in the left eye at 14 months after the injury. The analysis showed that the cone mosaic (cone density, 16503/mm(2); ratio of hexagonal Voronoi domain, 36.3 %; average nearest-neighbor distance (NND)/expected NND, 0.606) was disordered compared with the normal area of the same eye (cone density, 24821/mm(2); ratio of hexagonal Voronoi domain, 44.1 %; average NND/expected NND, 0.739). The cone defect area corresponded to the area of the scotoma. A second AO-SLO was performed on the patient at 22 months after the injury and although there were still areas with reduced cone reflectivity, partial improvement of cone mosaic was detected by AO-SLO at this time point. Partial recovery of damaged cone photoreceptors following closed globe blunt ocular trauma can be documented using AO-SLO longitudinal tracking.

  10. Principles of adaptive optics

    CERN Document Server

    Tyson, Robert

    2010-01-01

    History and BackgroundIntroductionHistoryPhysical OpticsTerms in Adaptive OpticsSources of AberrationsAtmospheric TurbulenceThermal BloomingNonatmospheric SourcesAdaptive Optics CompensationPhase ConjugationLimitations of Phase ConjugationArtificial Guide StarsLasers for Guide StarsCombining the LimitationsLinear AnalysisPartial Phase ConjugationAdaptive Optics SystemsAdaptive Optics Imaging SystemsBeam Propagation Syst

  11. Adaptive Optical Burst Switching

    OpenAIRE

    Bonald, Thomas; Indre, Raluca-Maria; Oueslati, Sara

    2012-01-01

    International audience; We propose a modified version of Optical Burst Switching (OBS) that adapts the size of switched data units to the network load. Specifically, we propose a two-way reservation OBS scheme in which every active source-destination pair attempts to reserve a lightpath and for every successful reservation, transmits an optical burst whose size is proportional to the number of active data flows. We refer to this technique as Adaptive Optical Burst Switching. We prove that the...

  12. Phase sensitive scanning optical microscope

    Energy Technology Data Exchange (ETDEWEB)

    Jungerman, R.L.; Hobbs, P.C.D.; Kino, G.S.

    1984-10-15

    An electronically scanned optical microscope which quantitatively measures amplitude and phase is described. The system is insenstive to mechanical vibrations. The phase infromation makes it possible to measure surface height variations with an accuracy of better than 100 A and can also be used to improve the lateral resolution.

  13. Adaptive optical filtering techniques

    Science.gov (United States)

    Psaltis, D.

    1985-05-01

    The purpose of this study was to examine the potential of using optical information processing technology for adaptive antenna beamforming and null steering. The adaptive beamforming/null steering problem consists of estimation of the covariance matrix of the noise field and inversion of the covariance matrix to obtain the antenna element weights which optimize the antenna's directional characteristics (gain pattern). This report examines the adaptive beamforming/nulling problem in view of the capabilities of optics and identifies areas where optics can be used to benefit. Benefits and drawbacks of various optical implementations of open and closed loop adaptive algorithms are discussed as well as the issues involved with optically processing digital binary numbers.

  14. Adaptive optics in ophthalmology

    Science.gov (United States)

    Iroshnikov, Nikita G.; Larichev, Andrey V.

    2006-09-01

    We present the experimental implementation of ophthalmic diagnostic systems with adaptive optics compensation of human eye aberration. The systems feature high speed operation and utilize deformable bimorph mirrors for wavefront correction. The results of aberration measurements and correction are discussed.

  15. Solar tomography adaptive optics.

    Science.gov (United States)

    Ren, Deqing; Zhu, Yongtian; Zhang, Xi; Dou, Jiangpei; Zhao, Gang

    2014-03-10

    Conventional solar adaptive optics uses one deformable mirror (DM) and one guide star for wave-front sensing, which seriously limits high-resolution imaging over a large field of view (FOV). Recent progress toward multiconjugate adaptive optics indicates that atmosphere turbulence induced wave-front distortion at different altitudes can be reconstructed by using multiple guide stars. To maximize the performance over a large FOV, we propose a solar tomography adaptive optics (TAO) system that uses tomographic wave-front information and uses one DM. We show that by fully taking advantage of the knowledge of three-dimensional wave-front distribution, a classical solar adaptive optics with one DM can provide an extra performance gain for high-resolution imaging over a large FOV in the near infrared. The TAO will allow existing one-deformable-mirror solar adaptive optics to deliver better performance over a large FOV for high-resolution magnetic field investigation, where solar activities occur in a two-dimensional field up to 60'', and where the near infrared is superior to the visible in terms of magnetic field sensitivity.

  16. Solar Adaptive Optics

    Directory of Open Access Journals (Sweden)

    Thomas R. Rimmele

    2011-06-01

    Full Text Available Adaptive optics (AO has become an indispensable tool at ground-based solar telescopes. AO enables the ground-based observer to overcome the adverse effects of atmospheric seeing and obtain diffraction limited observations. Over the last decade adaptive optics systems have been deployed at major ground-based solar telescopes and revitalized ground-based solar astronomy. The relatively small aperture of solar telescopes and the bright source make solar AO possible for visible wavelengths where the majority of solar observations are still performed. Solar AO systems enable diffraction limited observations of the Sun for a significant fraction of the available observing time at ground-based solar telescopes, which often have a larger aperture than equivalent space based observatories, such as HINODE. New ground breaking scientific results have been achieved with solar adaptive optics and this trend continues. New large aperture telescopes are currently being deployed or are under construction. With the aid of solar AO these telescopes will obtain observations of the highly structured and dynamic solar atmosphere with unprecedented resolution. This paper reviews solar adaptive optics techniques and summarizes the recent progress in the field of solar adaptive optics. An outlook to future solar AO developments, including a discussion of Multi-Conjugate AO (MCAO and Ground-Layer AO (GLAO will be given.

  17. Advanced Adaptive Optics Technology Development

    Energy Technology Data Exchange (ETDEWEB)

    Olivier, S

    2001-09-18

    The NSF Center for Adaptive Optics (CfAO) is supporting research on advanced adaptive optics technologies. CfAO research activities include development and characterization of micro-electro-mechanical systems (MEMS) deformable mirror (DM) technology, as well as development and characterization of high-resolution adaptive optics systems using liquid crystal (LC) spatial light modulator (SLM) technology. This paper presents an overview of the CfAO advanced adaptive optics technology development activities including current status and future plans.

  18. Science with Adaptive Optics

    CERN Document Server

    Brandner, Wolfgang; ESO Workshop

    2005-01-01

    The field of Adaptive Optics (AO) for astronomy has matured in recent years, and diffraction-limited image resolution in the near-infrared is now routinely achieved by ground-based 8 to 10m class telescopes. This book presents the proceedings of the ESO Workshop on Science with Adaptive Optics held in the fall of 2003. The book provides an overview on AO instrumentation, data acquisition and reduction strategies, and covers observations of the sun, solar system objects, circumstellar disks, substellar companions, HII regions, starburst environments, late-type stars, the galactic center, active galaxies, and quasars. The contributions present a vivid picture of the multitude of science topics being addressed by AO in observational astronomy.

  19. Optical Profilometers Using Adaptive Signal Processing

    Science.gov (United States)

    Hall, Gregory A.; Youngquist, Robert; Mikhael, Wasfy

    2006-01-01

    A method of adaptive signal processing has been proposed as the basis of a new generation of interferometric optical profilometers for measuring surfaces. The proposed profilometers would be portable, hand-held units. Sizes could be thus reduced because the adaptive-signal-processing method would make it possible to substitute lower-power coherent light sources (e.g., laser diodes) for white light sources and would eliminate the need for most of the optical components of current white-light profilometers. The adaptive-signal-processing method would make it possible to attain scanning ranges of the order of decimeters in the proposed profilometers.

  20. New Adaptive Optics Technique Demonstrated

    Science.gov (United States)

    2007-03-01

    Enrico Marchetti, the MAD Project Manager. "The system behaviour was very stable and the acquisition and closed loop operations were fast and smooth." ESO PR Photo 19d/07 ESO PR Photo 19d/07 AO Strehl Maps After routine checks on the closed loop stability and preliminary scans of the system parameters, the telescope was pointed to Omega Centauri, a very crowded area in the sky, and an optimal test case for extracting accurate measurements on AO correction performance with good spatial resolution on the FoV. Three 11 magnitude stars within a circle of ~1.5 arcmin diameter were selected as the baseline for wavefront sensing and the MCAO loop was closed successfully. Omega Centauri will be observed for several nights more, in order to test the AO correction in different seeing conditions. "This is a tremendous achievement that opens new perspectives in the era of extremely large telescopes," said Catherine Cesarsky, ESO's Director General. " "I am very proud of the ESO staff and wish to congratulate all involved for their prowess," she added. The MAD images perfectly show the validity of the concept. The image quality was almost uniform over the whole field of view and beautifully corrected for some of the atmospheric turbulence. More Information The Multi-Conjugate Adaptive Optics (MCAO) Demonstrator MAD was built by ESO in collaboration with the Astronomical Observatories of Arcetri and Padova (Italy) and the Faculdade de Ciencias da Universidade de Lisboa (Portugal), as a pathfinder for 2nd generation VLT instrumentation and the European Extremely Large Telescope project. The MCAO technique is based on probing the atmospheric turbulence on a large volume of atmosphere by means of several wavefront sensors (WFS), which point at different locations in the observed field of view, and by means of several deformable mirrors - optically conjugated at different altitudes on the atmosphere above the telescope - which correct for the atmospheric disturbance. The signals provided

  1. The research and development of the adaptive optics in ophthalmology

    Science.gov (United States)

    Wu, Chuhan; Zhang, Xiaofang; Chen, Weilin

    2015-08-01

    Recently the combination of adaptive optics and ophthalmology has made great progress and become highly effective. The retina disease is diagnosed by retina imaging technique based on scanning optical system, so the scanning of eye requires optical system characterized by great ability of anti-moving and optical aberration correction. The adaptive optics possesses high level of adaptability and is available for real time imaging, which meets the requirement of medical retina detection with accurate images. Now the Scanning Laser Ophthalmoscope and the Optical Coherence Tomography are widely used, which are the core techniques in the area of medical retina detection. Based on the above techniques, in China, a few adaptive optics systems used for eye medical scanning have been designed by some researchers from The Institute of Optics And Electronics of CAS(The Chinese Academy of Sciences); some foreign research institutions have adopted other methods to eliminate the interference of eye moving and optical aberration; there are many relevant patents at home and abroad. In this paper, the principles and relevant technique details of the Scanning Laser Ophthalmoscope and the Optical Coherence Tomography are described. And the recent development and progress of adaptive optics in the field of eye retina imaging are analyzed and summarized.

  2. Adaptive optical zoom sensor.

    Energy Technology Data Exchange (ETDEWEB)

    Sweatt, William C.; Bagwell, Brett E.; Wick, David Victor

    2005-11-01

    In order to optically vary the magnification of an imaging system, continuous mechanical zoom lenses require multiple optical elements and use fine mechanical motion to precisely adjust the separations between individual or groups of lenses. By incorporating active elements into the optical design, we have designed and demonstrated imaging systems that are capable of variable optical magnification with no macroscopic moving parts. Changing the effective focal length and magnification of an imaging system can be accomplished by adeptly positioning two or more active optics in the optical design and appropriately adjusting the optical power of those elements. In this application, the active optics (e.g. liquid crystal spatial light modulators or deformable mirrors) serve as variable focal-length lenses. Unfortunately, the range over which currently available devices can operate (i.e. their dynamic range) is relatively small. Therefore, the key to this concept is to create large changes in the effective focal length of the system with very small changes in the focal lengths of individual elements by leveraging the optical power of conventional optical elements surrounding the active optics. By appropriately designing the optical system, these variable focal-length lenses can provide the flexibility necessary to change the overall system focal length, and therefore magnification, that is normally accomplished with mechanical motion in conventional zoom lenses.

  3. Maritime adaptive optics beam control

    OpenAIRE

    Corley, Melissa S.

    2010-01-01

    The Navy is interested in developing systems for horizontal, near ocean surface, high-energy laser propagation through the atmosphere. Laser propagation in the maritime environment requires adaptive optics control of aberrations caused by atmospheric distortion. In this research, a multichannel transverse adaptive filter is formulated in Matlab's Simulink environment and compared to a complex lattice filter that has previously been implemented in large system simulations. The adaptive fil...

  4. Adaptive Optics for Large Telescopes

    Energy Technology Data Exchange (ETDEWEB)

    Olivier, S

    2008-06-27

    The use of adaptive optics was originally conceived by astronomers seeking to correct the blurring of images made with large telescopes due to the effects of atmospheric turbulence. The basic idea is to use a device, a wave front corrector, to adjust the phase of light passing through an optical system, based on some measurement of the spatial variation of the phase transverse to the light propagation direction, using a wave front sensor. Although the original concept was intended for application to astronomical imaging, the technique can be more generally applied. For instance, adaptive optics systems have been used for several decades to correct for aberrations in high-power laser systems. At Lawrence Livermore National Laboratory (LLNL), the world's largest laser system, the National Ignition Facility, uses adaptive optics to correct for aberrations in each of the 192 beams, all of which must be precisely focused on a millimeter scale target in order to perform nuclear physics experiments.

  5. Adaptive Optics for Industry and Medicine

    Science.gov (United States)

    Dainty, Christopher

    2008-01-01

    wavefront corrector ophthalmic adaptive optics: design and alignment (oral paper) / Alfredo Dubra and David Williams. High speed simultaneous SLO/OCT imaging of the human retina with adaptive optics (oral paper) / M. Pircher ... [et al.]. Characterization of an AO-OCT system (oral paper) / Julia W. Evans ... [et al.]. Adaptive optics optical coherence tomography for retina imaging (oral paper) / Guohua Shi ... [et al.]. Development, calibration and performance of an electromagnetic-mirror-based adaptive optics system for visual optics (oral paper) / Enrique Gambra ... [et al.]. Adaptive eye model (poster paper) / Sergey O. Galetskzy and Alexty V. Kudryashov. Adaptive optics system for retinal imaging based on a pyramid wavefront sensor (poster paper) / Sabine Chiesa ... [et al.]. Modeling of non-stationary dynamic ocular aberrations (poster paper) / Conor Leahy and Chris Dainty. High-order aberrations and accommodation of human eye (poster paper) / Lixia Xue ... [et al.]. Electromagnetic deformable mirror: experimental assessment and first ophthalmic applications (poster paper) / L. Vabre ... [et al.]. Correcting ocular aberrations in optical coherence tomography (poster paper) / Simon Tuohy ... [et al.] -- pt. 4. Adaptive optics in optical storage and microscopy. The application of liquid crystal aberration compensator for the optical disc systems (invited paper) / Masakazu Ogasawara. Commercialization of the adaptive scanning optical microscope (ASOM) (oral paper) / Benjamin Potsaid ... [et al.]. A practical implementation of adaptive optics for aberration compensation in optical microscopy (oral paper) / A. J. Wright ... [et al.]. Active focus locking in an optically sectioning microscope using adaptive optics (poster paper) / S. Poland, A. J. Wright, J. M. Girkin. Towards four dimensional particle tracking for biological applications / Heather I. Campbell ... [et al.]. Adaptive optics for microscopy (poster paper) / Xavier Levecq -- pt. 5. Adaptive optics in lasers

  6. Intelligent Optical Systems Using Adaptive Optics

    Science.gov (United States)

    Clark, Natalie

    2012-01-01

    Until recently, the phrase adaptive optics generally conjured images of large deformable mirrors being integrated into telescopes to compensate for atmospheric turbulence. However, the development of smaller, cheaper devices has sparked interest for other aerospace and commercial applications. Variable focal length lenses, liquid crystal spatial light modulators, tunable filters, phase compensators, polarization compensation, and deformable mirrors are becoming increasingly useful for other imaging applications including guidance navigation and control (GNC), coronagraphs, foveated imaging, situational awareness, autonomous rendezvous and docking, non-mechanical zoom, phase diversity, and enhanced multi-spectral imaging. The active components presented here allow flexibility in the optical design, increasing performance. In addition, the intelligent optical systems presented offer advantages in size and weight and radiation tolerance.

  7. Optical advantages in retinal scanning displays

    Science.gov (United States)

    Urey, Hakan

    2000-06-01

    Virtual Retinal DisplayTM technology is a retinal scanning display (RSD) technology being developed at Microvision, Inc., for a variety of applications including microdisplays. An RSD scans a modulated light beam onto a viewer's retina to produce a perceived image. Red, green and blue light sources, such as lasers, laser diodes or LEDs combine with Microvision's proprietary miniaturized scanner designs to make the RSD very well suited for head-worn and helmet-mounted displays (HMD). This paper compares the features of RSD technology to other display technologies such as the cathode ray tubes or matrix-based displays for HMD and other wearable display applications, and notes important performance advantages due to the number of pixel- generating elements. Also discussed are some fundamental optical limitations for virtual displays used in the HMD applications.

  8. Cornea Optical Topographical Scan System (COTSS)

    Science.gov (United States)

    1986-01-01

    The Cornea Optical Topographical Scan System (COTSS) is an instrument designed for use by opthalmologist to aid in performing surgical procedures such as radial keratotomy and to provide quick accurate data to aid in prescribing contact lenses and eyeglasses. A breadboard of the system was built and demonstrated in June of 1984. Additional refinements to the breadboard are needed to meet systems requirements prior to proceeding with prototype development. The present status of the COTSS instrument is given and the areas in which system refinements are required, are defined.

  9. Adaptive Optics Metrics & QC Scheme

    Science.gov (United States)

    Girard, Julien H.

    2017-09-01

    "There are many Adaptive Optics (AO) fed instruments on Paranal and more to come. To monitor their performances and assess the quality of the scientific data, we have developed a scheme and a set of tools and metrics adapted to each flavour of AO and each data product. Our decisions to repeat observations or not depends heavily on this immediate quality control "zero" (QC0). Atmospheric parameters monitoring can also help predict performances . At the end of the chain, the user must be able to find the data that correspond to his/her needs. In Particular, we address the special case of SPHERE."

  10. Gold nanocone near-field scanning optical microscopy probes.

    Science.gov (United States)

    Fleischer, Monika; Weber-Bargioni, Alexander; Altoe, M Virginia P; Schwartzberg, Adam M; Schuck, P James; Cabrini, Stefano; Kern, Dieter P

    2011-04-26

    Near-field scanning optical microscopy enables the simultaneous topographical and subdiffraction limited optical imaging of surfaces. A process is presented for the implementation of single individually engineered gold cones at the tips of atomic force microscopy cantilevers. These cantilevers act as novel high-performance optical near-field probes. In the fabrication, thin-film metallization, electron beam induced deposition of etch masks, and Ar ion milling are combined. The cone constitutes a well-defined highly efficient optical antenna with a tip radius on the order of 10 nm and an adjustable plasmon resonance frequency. The sharp tip enables high resolution topographical imaging. By controllably varying the cone size, the resonance frequency can be adapted to the application of choice. Structural properties of these sharp-tipped probes are presented together with topographical images recorded with a cone probe. The antenna functionality is demonstrated by gathering the near-field enhanced Raman signature of individual carbon nanotubes with a gold cone scanning probe.

  11. Adaptive optical probe design for optical coherence tomography and microscopy using tunable optics.

    Science.gov (United States)

    Choi, Minseog; Lee, Seungwan; Chang, Jong-Hyeon; Lee, Eunsung; Jung, Kyu-Dong; Kim, Woonbae

    2013-01-28

    We present a tunable, adaptive optical imaging probe for multimodal imaging such as optical coherence tomography and microscopy. The probe is compatible with forward-looking scanning laser imaging devices such as an endoscope. The lens configuration includes a tunable iris and two varifocal lenses, both driven by microelectrofluidics, as well as several conventional fixed focus lenses. The modulation transfer function and spot size in the focal plane is evaluated, and we show using optical simulations that there are three possible imaging modes with different transverse resolutions and focal depths.

  12. New scanning technique for the optical vortex microscope.

    Science.gov (United States)

    Augustyniak, Ireneusz; Popiołek-Masajada, Agnieszka; Masajada, Jan; Drobczyński, Sławomir

    2012-04-01

    In the optical vortex microscopy the focused Gaussian beam with optical vortex scans a sample. An optical vortex can be introduced into a laser beam with the use of a special optical element--a vortex lens. When moving the vortex lens, the optical vortex changes its position inside the spot formed by a focused laser beam. This effect can be used as a new precise scanning technique. In this paper, we study the optical vortex behavior at the sample plane. We also estimate if the new scanning technique results in observable effects that could be used for a phase object detection.

  13. Adaptive Optics Imaging in Laser Pointer Maculopathy.

    Science.gov (United States)

    Sheyman, Alan T; Nesper, Peter L; Fawzi, Amani A; Jampol, Lee M

    2016-08-01

    The authors report multimodal imaging including adaptive optics scanning laser ophthalmoscopy (AOSLO) (Apaeros retinal image system AOSLO prototype; Boston Micromachines Corporation, Boston, MA) in a case of previously diagnosed unilateral acute idiopathic maculopathy (UAIM) that demonstrated features of laser pointer maculopathy. The authors also show the adaptive optics images of a laser pointer maculopathy case previously reported. A 15-year-old girl was referred for the evaluation of a maculopathy suspected to be UAIM. The authors reviewed the patient's history and obtained fluorescein angiography, autofluorescence, optical coherence tomography, infrared reflectance, and AOSLO. The time course of disease and clinical examination did not fit with UAIM, but the linear pattern of lesions was suspicious for self-inflicted laser pointer injury. This was confirmed on subsequent questioning of the patient. The presence of linear lesions in the macula that are best highlighted with multimodal imaging techniques should alert the physician to the possibility of laser pointer injury. AOSLO further characterizes photoreceptor damage in this condition. [Ophthalmic Surg Lasers Imaging Retina. 2016;47:782-785.]. Copyright 2016, SLACK Incorporated.

  14. Novel optical scanning cryptography using Fresnel telescope imaging.

    Science.gov (United States)

    Yan, Aimin; Sun, Jianfeng; Hu, Zhijuan; Zhang, Jingtao; Liu, Liren

    2015-07-13

    We propose a new method called modified optical scanning cryptography using Fresnel telescope imaging technique for encryption and decryption of remote objects. An image or object can be optically encrypted on the fly by Fresnel telescope scanning system together with an encryption key. For image decryption, the encrypted signals are received and processed with an optical coherent heterodyne detection system. The proposed method has strong performance through use of secure Fresnel telescope scanning with orthogonal polarized beams and efficient all-optical information processing. The validity of the proposed method is demonstrated by numerical simulations and experimental results.

  15. Wavefront sensorless adaptive optics ophthalmoscopy in the human eye

    Science.gov (United States)

    Hofer, Heidi; Sredar, Nripun; Queener, Hope; Li, Chaohong; Porter, Jason

    2011-01-01

    Wavefront sensor noise and fidelity place a fundamental limit on achievable image quality in current adaptive optics ophthalmoscopes. Additionally, the wavefront sensor ‘beacon’ can interfere with visual experiments. We demonstrate real-time (25 Hz), wavefront sensorless adaptive optics imaging in the living human eye with image quality rivaling that of wavefront sensor based control in the same system. A stochastic parallel gradient descent algorithm directly optimized the mean intensity in retinal image frames acquired with a confocal adaptive optics scanning laser ophthalmoscope (AOSLO). When imaging through natural, undilated pupils, both control methods resulted in comparable mean image intensities. However, when imaging through dilated pupils, image intensity was generally higher following wavefront sensor-based control. Despite the typically reduced intensity, image contrast was higher, on average, with sensorless control. Wavefront sensorless control is a viable option for imaging the living human eye and future refinements of this technique may result in even greater optical gains. PMID:21934779

  16. Scanning optical microscope with long working distance objective

    Science.gov (United States)

    Cloutier, Sylvain G.

    2010-10-19

    A scanning optical microscope, including: a light source to generate a beam of probe light; collimation optics to substantially collimate the probe beam; a probe-result beamsplitter; a long working-distance, infinity-corrected objective; scanning means to scan a beam spot of the focused probe beam on or within a sample; relay optics; and a detector. The collimation optics are disposed in the probe beam. The probe-result beamsplitter is arranged in the optical paths of the probe beam and the resultant light from the sample. The beamsplitter reflects the probe beam into the objective and transmits resultant light. The long working-distance, infinity-corrected objective is also arranged in the optical paths of the probe beam and the resultant light. It focuses the reflected probe beam onto the sample, and collects and substantially collimates the resultant light. The relay optics are arranged to relay the transmitted resultant light from the beamsplitter to the detector.

  17. ADAPTIVE OPTICAL SYSTEMS LIGHTING EQUIPMENT VEHICLES

    OpenAIRE

    S. P. Sernov; D. V. Balokhonov; T. V. Kolontaeva; A. V. Zhuravok

    2011-01-01

    This article describes the main principles of implementation of modern adaptive signal lighting equipment of vehicles, provides an analysis of optical systems are used, the necessity of the use of LEDs. We present the design of adaptive optical system, rear combination LED lamp of a vehicle with several levels of intensity, we discuss the algorithm of its work in different modes. 

  18. Adaptive optics imaging of the retina

    Directory of Open Access Journals (Sweden)

    Rajani Battu

    2014-01-01

    Full Text Available Adaptive optics is a relatively new tool that is available to ophthalmologists for study of cellular level details. In addition to the axial resolution provided by the spectral-domain optical coherence tomography, adaptive optics provides an excellent lateral resolution, enabling visualization of the photoreceptors, blood vessels and details of the optic nerve head. We attempt a mini review of the current role of adaptive optics in retinal imaging. PubMed search was performed with key words Adaptive optics OR Retina OR Retinal imaging. Conference abstracts were searched from the Association for Research in Vision and Ophthalmology (ARVO and American Academy of Ophthalmology (AAO meetings. In total, 261 relevant publications and 389 conference abstracts were identified.

  19. Second-harmonic scanning optical microscopy of semiconductor quantum dots

    DEFF Research Database (Denmark)

    Vohnsen, B.; Bozhevolnyi, S.I.; Pedersen, K.

    2001-01-01

    Second-harmonic (SH) optical imaging of self-assembled InAlGaAs quantum dots (QD's) grown on a GaAs(0 0 1) substrate has been accomplished at room temperature by use of respectively a scanning far-field optical microscope in reflection mode and a scanning near-field optical microscope...... in transmission mode. In both cases the SH signal peaks at a pump wavelength of similar to 885 nm in correspondence to the maximum in the photoluminescence spectrum of the QD sample. SH near-field optical images exhibit spatial signal variations on a subwavelength scale that depend on the pump wavelength. We...

  20. Meaning of visualizing retinal cone mosaic on adaptive optics images.

    Science.gov (United States)

    Jacob, Julie; Paques, Michel; Krivosic, Valérie; Dupas, Bénédicte; Couturier, Aude; Kulcsar, Caroline; Tadayoni, Ramin; Massin, Pascale; Gaudric, Alain

    2015-01-01

    To explore the anatomic correlation of the retinal cone mosaic on adaptive optics images. Retrospective nonconsecutive observational case series. A retrospective review of the multimodal imaging charts of 6 patients with focal alteration of the cone mosaic on adaptive optics was performed. Retinal diseases included acute posterior multifocal placoid pigment epitheliopathy (n = 1), hydroxychloroquine retinopathy (n = 1), and macular telangiectasia type 2 (n = 4). High-resolution retinal images were obtained using a flood-illumination adaptive optics camera. Images were recorded using standard imaging modalities: color and red-free fundus camera photography; infrared reflectance scanning laser ophthalmoscopy, fluorescein angiography, indocyanine green angiography, and spectral-domain optical coherence tomography (OCT) images. On OCT, in the marginal zone of the lesions, a disappearance of the interdigitation zone was observed, while the ellipsoid zone was preserved. Image recording demonstrated that such attenuation of the interdigitation zone co-localized with the disappearance of the cone mosaic on adaptive optics images. In 1 case, the restoration of the interdigitation zone paralleled that of the cone mosaic after a 2-month follow-up. Our results suggest that the interdigitation zone could contribute substantially to the reflectance of the cone photoreceptor mosaic. The absence of cones on adaptive optics images does not necessarily mean photoreceptor cell death. Copyright © 2015 Elsevier Inc. All rights reserved.

  1. Adaptive phase compensation for ultracompact laser scanning endomicroscopy.

    Science.gov (United States)

    Thompson, Alex J; Paterson, Carl; Neil, Mark A A; Dunsby, Chris; French, Paul M W

    2011-05-01

    We present an approach to laser scanning endomicroscopy that requires no moving parts and can be implemented with no distal scanners or optics, permitting extremely compact endoscopic probes to be developed. Our approach utilizes a spatial light modulator to correct for phase variations across a fiber imaging bundle and to encode for arbitrary wavefronts at the distal end of the fiber bundle. Thus, it is possible to realize both focusing and beam scanning at the output of the fiber bundle with no distal components. We present proof of principle results to illustrate three-dimensional scanning of the focal spot and exemplar images of a United States Air Force resolution test chart.

  2. Holographic Video Disc And Laser Scanning Optics.

    Science.gov (United States)

    Weingartner, I.; Rosenbruch, K. J.

    1983-10-01

    Holographic optical elements or systems of holographic elements may replace glass optical imaging systems or may be used for the correction of glass optics. The main advantages of such systems are their low weight, small and compact construction, and their simple and inexpensive manufacture. The disadvantages to be overcome are mainly the low light through-put and chromatic aberrations. In the special case of optics for video discs we present an optical imaging system which is capable of giving the required high resolution for illumination with polychromatic radiation of limited bandwidth in the case of semiconductor laser diodes. Optimization programs based on ray tracing yield highly corrected imaging systems by comparably simple holographic means. The use of only two surfaces gives very compact and lightweight systems, the image quality of which is described for monochromatic and polychro-matic irradiance by means of optical transfer functions. The holograms are recorded on photo-resist material with short wavelength laser radiation. Such holograms have almost no scatter light and do not alter their properties with time or under radiation. These holograms generate wavefronts for the correction of aberrations which, in the case of glass optics, could only be achieved by aspherical surfaces.

  3. Teaching Optics and Systems Engineering With Adaptive Optics Workbenches

    CERN Document Server

    Harrington, David; Hunter, Lisa; Max, Claire; Hoffmann, Mark; Pitts, Mark; Armstrong, J D

    2010-01-01

    Adaptive optics workbenches are fully functional optical systems that can be used to illustrate and teach a variety of concepts and cognitive processes. Four systems have been funded, designed and constructed by various institutions and people as part of education programs associated with the Center for Adaptive Optics, the Professional Development Program and the Institute for Science and Engineer Educators. Activities can range from first-year undergraduate explorations to professional level training. These workbenches have been used in many venues including the Center for Adaptive Optics AO Summer School, the Maui Community College hosted Akamai Maui Short Course, classrooms, training of new staff in laboratories and other venues. The activity content has focused on various elements of systems thinking, characterization, feedback and system control, basic optics and optical alignment as well as advanced topics such as phase conjugation, wave-front sensing and correction concepts and system design. The work...

  4. Atmospheric free-space coherent optical communications with adaptive optics

    Science.gov (United States)

    Ting, Chueh; Zhang, Chengyu; Yang, Zikai

    2017-02-01

    Free-space coherent optical communications have a potential application to offer last mile bottleneck solution in future local area networks (LAN) because of their information carrier, information security and license-free status. Coherent optical communication systems using orthogonal frequency division multiplexing (OFDM) digital modulation are successfully demonstrated in a long-haul tens Giga bits via optical fiber, but they are not yet available in free space due to atmospheric turbulence-induced channel fading. Adaptive optics is recognized as a promising technology to mitigate the effects of atmospheric turbulence in free-space optics. In this paper, a free-space coherent optical communication system using an OFDM digital modulation scheme and adaptive optics (FSO OFDM AO) is proposed, a Gamma-Gamma distribution statistical channel fading model for the FSO OFDM AO system is examined, and FSO OFDM AO system performance is evaluated in terms of bit error rate (BER) versus various propagation distances.

  5. Optical characterication of probes for photon scanning tunnelling microscopy

    DEFF Research Database (Denmark)

    Vohnsen, Brian; Bozhevolnyi, Sergey I.

    1999-01-01

    The photon scanning tunnelling microscope is a well-established member of the family of scanning near-field optical microscopes used for optical imaging at the sub-wavelength scale. The quality of the probes, typically pointed uncoated optical fibres, used is however difficult to evaluate...... in a direct manner and has most often been inferred from the apparent quality of recorded optical images. Complicated near-field optical imaging characteristics, together with the possibility of topographically induced artefacts, however, has increased demands for a more reliable probe characterization...... technique. Here we present experimental results obtained for optical characterization of two different probes by imaging of a well-specified near-field intensity distribution at various spatial frequencies. In particular, we observe that a sharply pointed dielectric probe can be highly suitable for imaging...

  6. Video rate near-field scanning optical microscopy

    Science.gov (United States)

    Bukofsky, S. J.; Grober, R. D.

    1997-11-01

    The enhanced transmission efficiency of chemically etched near-field optical fiber probes makes it possible to greatly increase the scanning speed of near-field optical microscopes. This increase in system bandwidth allows sub-diffraction limit imaging of samples at video rates. We demonstrate image acquisition at 10 frames/s, rate-limited by mechanical resonances in our scanner. It is demonstrated that the optical signal to noise ratio is large enough for megahertz single pixel acquisition rates.

  7. ADAPTIVE OPTICAL SYSTEMS LIGHTING EQUIPMENT VEHICLES

    Directory of Open Access Journals (Sweden)

    S. P. Sernov

    2011-01-01

    Full Text Available This article describes the main principles of implementation of modern adaptive signal lighting equipment of vehicles, provides an analysis of optical systems are used, the necessity of the use of LEDs. We present the design of adaptive optical system, rear combination LED lamp of a vehicle with several levels of intensity, we discuss the algorithm of its work in different modes. 

  8. A Miniaturized Adaptive Optic Device for Optical Telecommunications Project

    Data.gov (United States)

    National Aeronautics and Space Administration — To advance the state-of-the-art uplink laser communication technology, new adaptive optic beam compensation techniques are needed for removing various time-varying...

  9. Innovative advanced occlusion planning with superimposed CT and optical scans.

    Science.gov (United States)

    Tremblay, Gilbert

    2011-04-01

    In order to increase the likelihood of a successful treatment plan outcome, it is critical to be able to effectively view the patient's underlying bony skeletal relationship of his or her TMJ. An innovative approach suggested to achieve this is to use the CT scan, optical scan, and Kois deprogrammer. Once the vertical dimension has been increased, the novelty of this approach is the ability to superimpose both scans along with the Kois deprogrammer and, using computer software, evaluate the TMJ position in three dimensions. This case presentation describes how TMJ CT scan evaluation is used in planning a complex rehabilitation case, given that the occlusion structures can be visualized independently and interactively.

  10. Nanometrology using a through-focus scanning optical microscopy method

    Science.gov (United States)

    Attota, Ravikiran; Silver, Richard

    2011-02-01

    We present an initial review of a novel through-focus scanning optical microscopy (TSOM pronounced as 'tee-som') imaging method that produces nanometer-dimensional measurement sensitivity using a conventional bright-field optical microscope. In the TSOM method a target is scanned through the focus of an optical microscope, acquiring conventional optical images at different focal positions. The TSOM images are constructed using the through-focus optical images. A TSOM image is unique under given experimental conditions and is sensitive to changes in the dimensions of a target in a distinct way. We use this characteristic for nanoscale-dimensional metrology. This technique can be used to identify the dimension which is changing between two nanosized targets and to determine the dimensions using a library-matching method. This methodology has potential utility for a wide range of target geometries and application areas, including nanometrology, nanomanufacturing, defect analysis, inspection, process control and biotechnology.

  11. Adaptive Optics and NICMOS Uniqueness Space

    Energy Technology Data Exchange (ETDEWEB)

    Max, C.

    1999-03-22

    As part of the HST Second Decade Study a subgroup consisting of Claire Max, James Beletic, Donald McCarthy, and Keith Noll has analyzed the expected performance of near-infra-red adaptive optics systems on the new generation of 8-10 meter ground-based telescopes, for comparison with HST. In addition the subgroup has polled the adaptive optics community regarding expected adaptive optics performance over the coming five years. Responses have been received from representatives of most of the major telescopes: Gemini, VLT, Keck, LBT, and the MMT, as well as of several operational 3-4 meter telescope AO systems. The present document outlines the conclusions to date, with emphasis on aspects relevant to the NICMOS cryocooler Independent Science Review. In general the near-infra-red capabilities of the new ground-based adaptive optics systems will be complementary to the capabilities of NICMOS. For example NICMOS will have greater H-band sensitivity, broader wavelength coverage, and higher point-spread-function stability, whereas ground-based adaptive optics instruments will have higher spatial and spectral resolution. Section 2 of this report outlines the operational constraints faced by the first generation of adaptive optics (AO) systems on new 8-10 meter telescopes. Section 3 describes the areas of relative strength of near-infra-red observing from the ground via adaptive optics, compared with NICMOS. A Table gives an overview of the main strengths and weaknesses of these current-generation systems. Section 4 gives an indication of ground-based capabilities anticipated in the near future and in five to ten years. Section 5 contains a summary and conclusions.

  12. Implementation of 3D Optical Scanning Technology for Automotive Applications.

    Science.gov (United States)

    Kuş, Abdil

    2009-01-01

    Reverse engineering (RE) is a powerful tool for generating a CAD model from the 3D scan data of a physical part that lacks documentation or has changed from the original CAD design of the part. The process of digitizing a part and creating a CAD model from 3D scan data is less time consuming and provides greater accuracy than manually measuring the part and designing the part from scratch in CAD. 3D optical scanning technology is one of the measurement methods which have evolved over the last few years and it is used in a wide range of areas from industrial applications to art and cultural heritage. It is also used extensively in the automotive industry for applications such as part inspections, scanning of tools without CAD definition, scanning the casting for definition of the stock (i.e. the amount of material to be removed from the surface of the castings) model for CAM programs and reverse engineering. In this study two scanning experiments of automotive applications are illustrated. The first one examines the processes from scanning to re-manufacturing the damaged sheet metal cutting die, using a 3D scanning technique and the second study compares the scanned point clouds data to 3D CAD data for inspection purposes. Furthermore, the deviations of the part holes are determined by using different lenses and scanning parameters.

  13. Implementation of 3D Optical Scanning Technology for Automotive Applications

    Science.gov (United States)

    Kuş, Abdil

    2009-01-01

    Reverse engineering (RE) is a powerful tool for generating a CAD model from the 3D scan data of a physical part that lacks documentation or has changed from the original CAD design of the part. The process of digitizing a part and creating a CAD model from 3D scan data is less time consuming and provides greater accuracy than manually measuring the part and designing the part from scratch in CAD. 3D optical scanning technology is one of the measurement methods which have evolved over the last few years and it is used in a wide range of areas from industrial applications to art and cultural heritage. It is also used extensively in the automotive industry for applications such as part inspections, scanning of tools without CAD definition, scanning the casting for definition of the stock (i.e. the amount of material to be removed from the surface of the castings) model for CAM programs and reverse engineering. In this study two scanning experiments of automotive applications are illustrated. The first one examines the processes from scanning to re-manufacturing the damaged sheet metal cutting die, using a 3D scanning technique and the second study compares the scanned point clouds data to 3D CAD data for inspection purposes. Furthermore, the deviations of the part holes are determined by using different lenses and scanning parameters. PMID:22573995

  14. Real-time histogram generation using active optical scanning

    Science.gov (United States)

    Pieper, R. J.; Richstein, J. K.; Poon, T.-C.; Moore, D. J.

    1996-04-01

    A prototype of an optical-electronic histogram generator has been designed and tested for one-dimensional objects. In this scheme, the object to be analysed is laser scanned. The resulting optical signal is detected by a photodetector, which generates an electrical signal output that is subsequently analysed with a combination of analogue and digital electronics. The system is shown to be fairly modular in design. Various aspects of the extension of the design to two dimensions are discussed.

  15. Free Space Optical Communications Utilizing MEMS Adaptive Optics Correction

    Energy Technology Data Exchange (ETDEWEB)

    Thompson, C A; Kartz, M W; Flath, L M; Wilks, S C; Young, R A; Johnson, G W; Ruggiero, A J

    2002-07-09

    Free space optical communications (FSO) are beginning to provide attractive alternatives to fiber-based solutions in many situations. Currently, a handful of companies provide fiberless alternatives specifically aimed at corporate intranet and sporting event video applications. These solutions are geared toward solving the ''last mile'' connectivity issues. There exists a potential need to extend this pathlength to distances much greater than a 1 km, particularly for government and military applications. For cases of long distance optical propagation, atmospheric turbulence will ultimately limit the maximum achievable data rate. In this paper, we propose a method to improve signal quality through the use of adaptive optics. In particular, we show work in progress toward a high-speed, small footprint Adaptive Optics system for horizontal and slant path laser communications. Such a system relies heavily on recent progress in Micro-Electro-Mechanical Systems (MEMS) deformable mirrors, as well as improved communication and computational components.

  16. A High Speed Finger-Print Optical Scanning Method

    Science.gov (United States)

    2000-01-01

    counterfeiting in fingerprint identification can be easily ensured. These features are essential for fingerprint authentication in INTERNET environment...and E-Commerce environments. Keywords: fingerprint, optical scanning, plural LED, identification , E-commerce 1. INTRODUCTION Recently the biometrics...biometrics technologies for authentication, from the view point of convenience and higher security, dactyloscopy is by far the best, much better than the

  17. Optical video projection using laser beam scanning technology

    Science.gov (United States)

    Clynick, Tony J.

    1993-12-01

    Various techniques are currently used to project video images. One of these, described in a previous paper by the author, operates by mechanical scanning of a laser beam with acousto- optic modulation, and has been proven suitable for high definition television and computer display scan rates by use of novel electronic and optical compensation methods. The requirement for improved image intensity with greater efficiency has led to a re-appraisal of the selection of the light source and the relationship between the light source, the form of the modulator, and the method of scanning. The electrical input to visible radiation output ratio of the Argon-ion lasers currently used in the projector shows efficiency to be as low as 0.001%, a factor limiting the commercial exploitation of the projector. Recent developments in acousto- optics can be applied to the projector's optical system allowing alternative light sources to be used. These help to reduce the complexity of both the optical and signal processing stages as well as improve efficiency.

  18. Adaptive noise Wiener filter for scanning electron microscope imaging system.

    Science.gov (United States)

    Sim, K S; Teh, V; Nia, M E

    2016-01-01

    Noise on scanning electron microscope (SEM) images is studied. Gaussian noise is the most common type of noise in SEM image. We developed a new noise reduction filter based on the Wiener filter. We compared the performance of this new filter namely adaptive noise Wiener (ANW) filter, with four common existing filters as well as average filter, median filter, Gaussian smoothing filter and the Wiener filter. Based on the experiments results the proposed new filter has better performance on different noise variance comparing to the other existing noise removal filters in the experiments. © Wiley Periodicals, Inc.

  19. Contrast-based sensorless adaptive optics for retinal imaging.

    Science.gov (United States)

    Zhou, Xiaolin; Bedggood, Phillip; Bui, Bang; Nguyen, Christine T O; He, Zheng; Metha, Andrew

    2015-09-01

    Conventional adaptive optics ophthalmoscopes use wavefront sensing methods to characterize ocular aberrations for real-time correction. However, there are important situations in which the wavefront sensing step is susceptible to difficulties that affect the accuracy of the correction. To circumvent these, wavefront sensorless adaptive optics (or non-wavefront sensing AO; NS-AO) imaging has recently been developed and has been applied to point-scanning based retinal imaging modalities. In this study we show, for the first time, contrast-based NS-AO ophthalmoscopy for full-frame in vivo imaging of human and animal eyes. We suggest a robust image quality metric that could be used for any imaging modality, and test its performance against other metrics using (physical) model eyes.

  20. Adaptive Optics for the Human Eye

    Science.gov (United States)

    Williams, D. R.

    2000-05-01

    Adaptive optics can extend not only the resolution of ground-based telescopes, but also the human eye. Both static and dynamic aberrations in the cornea and lens of the normal eye limit its optical quality. Though it is possible to correct defocus and astigmatism with spectacle lenses, higher order aberrations remain. These aberrations blur vision and prevent us from seeing at the fundamental limits set by the retina and brain. They also limit the resolution of cameras to image the living retina, cameras that are a critical for the diagnosis and treatment of retinal disease. I will describe an adaptive optics system that measures the wave aberration of the eye in real time and compensates for it with a deformable mirror, endowing the human eye with unprecedented optical quality. This instrument provides fresh insight into the ultimate limits on human visual acuity, reveals for the first time images of the retinal cone mosaic responsible for color vision, and points the way to contact lenses and laser surgical methods that could enhance vision beyond what is currently possible today. Supported by the NSF Science and Technology Center for Adaptive Optics, the National Eye Institute, and Bausch and Lomb, Inc.

  1. Handheld probes and galvanometer scanning for optical coherence tomography

    Science.gov (United States)

    Duma, V.-F.; Dobre, G.; Demian, D.; Cernat, R.; Sinescu, C.; Topala, F. I.; Negrutiu, M. L.; Hutiu, Gh.; Bradu, A.; Rolland, J. P.; Podoleanu, A. G.

    2015-09-01

    As part of the ongoing effort of the biomedical imaging community to move Optical Coherence Tomography (OCT) systems from the lab to the clinical environment and produce OCT systems appropriate for multiple types of investigations in a medical department, handheld probes equipped with different types of scanners need to be developed. These allow different areas of a patient's body to be investigated using OCT with the same system and even without changing the patient's position. This paper reviews first the state of the art regarding OCT handheld probes. Novel probes with a uni-dimensional (1D) galvanometer-based scanner (GS) developed in our groups are presented. Their advantages and limitations are discussed. Aspects regarding the use of galvoscanners with regard to Micro-Electro- Mechanical Systems (MEMS) are pointed out, in relationship with our studies on optimal scanning functions of galvanometer devices in OCT. These scanning functions are briefly discussed with regard to their main parameters: profile, theoretical duty cycle, scan frequency, and scan amplitude. The optical design of the galvoscanner and refractive optics combination in the probe head, optimized for various applications, is considered. Perspectives of the field are pointed out in the final part of the paper.

  2. Optics designs and system MTF for laser scanning displays

    Science.gov (United States)

    Urey, Hakan; Nestorovic, Ned; Ng, Baldwin S.; Gross, Abraham A.

    1999-07-01

    The Virtual Retinal DisplayTM (VRDTM) technology is a new display technology being developed at Microvision Inc. The displayed image is scanned onto the viewer's retina using low- power red, green, and blue light sources. Microvision's proprietary miniaturized scanner designs make VRD system very well suited for head-mounted displays. In this paper we discuss some of the advantages of the VRD technology, various ocular designs for HMD and other applications, and details of constructing a system MTF budget for laser scanning systems that includes electronics, modulators, scanners, and optics.

  3. Transfer functions in collection scanning near-field optical microscopy

    DEFF Research Database (Denmark)

    Bozhevolnyi, Sergey I.; Vohnsen, Brian; Bozhevolnaya, Elena A.

    1999-01-01

    are considered with respect to the relation between near-field optical images and the corresponding intensity distributions. Our conclusions are supported with numerical simulations and experimental results obtained by using a photon scanning tunneling microscope with an uncoated fiber tip.......It is generally accepted that, if in collection near-field optical microscopy the probe-sample coupling can be disregarded, a fiber probe can be considered as a detector of the near-field intensity whose size can be accounted for via an intensity transfer function. We show that, in general...

  4. Processing of Graphene combining Optical Detection and Scanning Probe Lithography

    Directory of Open Access Journals (Sweden)

    Zimmermann Sören

    2015-01-01

    Full Text Available This paper presents an experimental setup tailored for robotic processing of graphene with in-situ vision based control. A robust graphene detection approach is presented applying multiple image processing operations of the visual feedback provided by a high-resolution light microscope. Detected graphene flakes can be modified using a scanning probe based lithographical process that is directly linked to the in-situ optical images. The results of this process are discussed with respect to further application scenarios.

  5. Adaptive optics in digital micromirror based confocal microscopy

    NARCIS (Netherlands)

    Pozzi, P.; Wilding, D.; Soloviev, O.A.; Vdovine, G.V.; Verhaegen, M.H.G.; Bifano, Thomas G.; Kubby, Joel; Gigan, Sylvain

    2016-01-01

    This proceeding reports early results in the development of a new technique for adaptive optics in confocal microscopy. The term adaptive optics refers to the branch of optics in which an active element in the optical system is used to correct inhomogeneities in the media through which light

  6. Operation of a scanning near field optical microscope in reflection in combination with a scanning force microscope

    NARCIS (Netherlands)

    van Hulst, N.F.; Moers, M.H.P.; Moers, M.H.P.; Noordman, O.F.J.; Noordman, O.F.J.; Faulkner, T.; Segerink, Franciscus B.; van der Werf, Kees; de Grooth, B.G.; Bölger, B.; Bölger, B.

    1992-01-01

    Images obtained with a scanning near field optical microscope (SNOM) operating in reflection are presented. We have obtained the first results with a SiN tip as optical probe. The instrument is simultaneously operated as a scanning force microscope (SFM). Moreover, the instrument incorporates an

  7. Specialized wavefront sensors for adaptive optics

    Energy Technology Data Exchange (ETDEWEB)

    Neal, D.R.; Mansell, J.D.; Gruetzner, J.K. [and others

    1995-08-01

    The performance of an adaptive optical system is strongly dependent upon correctly measuring the wavefront of the arriving light. The most common wavefront measurement techniques used to date are the shearing interferometer and the Shack-Hartmann sensor. Shack-Hartmann sensors rely on the use of lenslet arrays to sample the aperture appropriately. These have traditionally been constructed using ULM or step and repeat technology, and more recently with binary optics technology. Diffractive optics fabrication methodology can be used to remove some of the limitations of the previous technologies and can allow for low-cost production of sophisticated elements. We have investigated several different specialized wavefront sensor configurations using both Shack-Hartmann and shearing interferometer principles. We have taken advantage of the arbitrary nature of these elements to match pupil shapes of detector and telescope aperture and to introduce magnification between the lenslet array and the detector. We have fabricated elements that facilitate matching the sampling to the current atmospheric conditions. The sensors were designed using a far-field diffraction model and a photolithography layout program. They were fabricated using photolithography and RIE etching. Several different designs will be presented with some experimental results from a small-scale adaptive optics brass-board.

  8. Easy and versatile adaptive optics setup with deformable lens for high-resolution microscopy

    Science.gov (United States)

    Pozzi, P.; Quintavalla, M.; Verstraete, H.; Bijlsma, H.; Bonora, S.; Verhaegen, M.

    2017-06-01

    It has been widely proven in literature that most optical microscopy techniques can greatly benefit from the application of adaptive optics correction of phase aberrations through an adaptive optical element, such as a deformable mirror or a spatial light modulator. However, adaptive optics is not yet widely adopted in the life sciences community, mostly due to the lack of adaptive commercial microscopy systems, and the inherent technical difficulty in modifying an existing microscopy setup to integrate an adaptive element, both on the software and hardware sides. We present a plug-and-play adaptive optics module for generic optical microscopes, based on a prototype refractive 18 actuators adaptive optical element, which can be inserted in any microscope between the objective and the microscope body. Correction is performed in a sensorless fashion, optimizing image quality metrics of the image presented to the user on screen. The results presented show how an end-user oriented commercial confocal laser scanning microscope (Leica SP5) can be upgraded with adaptive optics with minor hardware modifications, and no changes to the microscope control software.

  9. In vivo cellular visualization of the human retina using optical coherence tomography and adaptive optics

    Energy Technology Data Exchange (ETDEWEB)

    Olivier, S S; Jones, S M; Chen, D C; Zawadzki, R J; Choi, S S; Laut, S P; Werner, J S

    2006-01-05

    Optical coherence tomography (OCT) sees the human retina sharply with adaptive optics. In vivo cellular visualization of the human retina at micrometer-scale resolution is possible by enhancing Fourier-domain optical-coherence tomography with adaptive optics, which compensate for the eye's optical aberrations.

  10. Scanning Long-wave Optical Test System: a new ground optical surface slope test system

    Science.gov (United States)

    Su, Tianquan; Park, Won Hyun; Parks, Robert E.; Su, Peng; Burge, James H.

    2011-09-01

    The scanning long-wave optical test system (SLOTS) is under development at the University of Arizona to provide rapid and accurate measurements of aspherical optical surfaces during the grinding stage. It is based on the success of the software configurable optical test system (SCOTS) which uses visible light to measure surface slopes. Working at long wave infrared (LWIR, 7-14 μm), SLOTS measures ground optical surface slopes by viewing the specular reflection of a scanning hot wire. A thermal imaging camera collects data while motorized stages scan the wire through the field. Current experiments show that the system can achieve a high precision at micro-radian level with fairly low cost equipment. The measured surface map is comparable with interferometer for slow optics. This IR system could be applied early in the grinding stage of fabrication of large telescope mirrors to minimize the surface shape error imparted during processing. This advantage combined with the simplicity of the optical system (no null optics, no high power carbon dioxide laser) would improve the efficiency and shorten the processing time.

  11. REVIEW ARTICLE: Scan-free optical correlation techniques: history and applications to optical coherence tomography

    Science.gov (United States)

    Froehly, Luc; Leitgeb, Rainer

    2010-08-01

    In parallel with progress in generating ultrafast pulse sources and characterization techniques, optical time correlation techniques have seen tremendous development over many years and paved the way for novel applications in non-destructive and high resolution 'optical coherence tomography' (OCT) imaging. Amongst the known correlation techniques, the scan-free approach presents the advantage of single shot detection and real-time acquisition for pulse measurements, but this is not generally considered and applied for OCT imaging. The aim of this paper is to review the scan-free correlation method, analyze its performance and extended features and discuss its application to OCT.

  12. A simple turbulence simulator for adaptive optics

    Science.gov (United States)

    Thomas, Sandrine

    2004-10-01

    In this article, I describe a new, inexpensive way to make transparent phase screens. I list available technologies of physical turbulence simulation and describe the transparent phase-plate screens that were produced by the laquer-spray technique and characterized in the laboratory. The spatial spectrum of phase perturbations is a reasonable match to the Kolmogorov law with r0 around 0.5 mm at 0.633 μm over spatial frequencies from 0.75 to 5 mm-1. A turbulence simulator using two such rotating screens and destined for the adaptive optics instrument for the 4.1-m SOAR telescope is described.

  13. Adaptive optics and laser guide stars at Lick observatory

    Energy Technology Data Exchange (ETDEWEB)

    Brase, J.M. [Lawrence Livermore National Lab., CA (United States)

    1994-11-15

    For the past several years LLNL has been developing adaptive optics systems for correction of both atmospheric turbulence effects and thermal distortions in optics for high-power lasers. Our early work focused on adaptive optics for beam control in laser isotope separation and ground-based free electron lasers. We are currently developing innovative adaptive optics and laser systems for sodium laser guide star applications at the University of California`s Lick and Keck Observeratories. This talk will describe our adaptive optics technology and some of its applications in high-resolution imaging and beam control.

  14. Optical design of the adaptive optics laser guide star system

    Energy Technology Data Exchange (ETDEWEB)

    Bissinger, H. [Lawrence Livermore National Lab., CA (United States)

    1994-11-15

    The design of an adaptive optics package for the 3 meter Lick telescope is presented. This instrument package includes a 69 actuator deformable mirror and a Hartmann type wavefront sensor operating in the visible wavelength; a quadrant detector for the tip-tile sensor and a tip-tilt mirror to stabilize atmospheric first order tip-tile errors. A high speed computer drives the deformable mirror to achieve near diffraction limited imagery. The different optical components and their individual design constraints are described. motorized stages and diagnostics tools are used to operate and maintain alignment throughout observation time from a remote control room. The expected performance are summarized and actual results of astronomical sources are presented.

  15. Optical microscope illumination analysis using through-focus scanning optical microscopy.

    Science.gov (United States)

    Attota, Ravi Kiran; Park, Haesung

    2017-06-15

    Misalignment of the aperture diaphragm present in optical microscopes results in angular illumination asymmetry (ANILAS) at the sample plane. Here we show that through-focus propagation of ANILAS results in a lateral image shift with a focus position. This could lead to substantial errors in quantitative results for optical methods that use through-focus images such as three-dimensional nanoparticle tracking, confocal microscopy, and through-focus scanning optical microscopy (TSOM). A correlation exists between ANILAS and the slant in TSOM images. Hence, the slant in the TSOM image can be used to detect, analyze, and rectify the presence of ANILAS.

  16. Extreme Adaptive Optics Planet Imager: XAOPI

    Energy Technology Data Exchange (ETDEWEB)

    Macintosh, B A; Graham, J; Poyneer, L; Sommargren, G; Wilhelmsen, J; Gavel, D; Jones, S; Kalas, P; Lloyd, J; Makidon, R; Olivier, S; Palmer, D; Patience, J; Perrin, M; Severson, S; Sheinis, A; Sivaramakrishnan, A; Troy, M; Wallace, K

    2003-09-17

    Ground based adaptive optics is a potentially powerful technique for direct imaging detection of extrasolar planets. Turbulence in the Earth's atmosphere imposes some fundamental limits, but the large size of ground-based telescopes compared to spacecraft can work to mitigate this. We are carrying out a design study for a dedicated ultra-high-contrast system, the eXtreme Adaptive Optics Planet Imager (XAOPI), which could be deployed on an 8-10m telescope in 2007. With a 4096-actuator MEMS deformable mirror it should achieve Strehl >0.9 in the near-IR. Using an innovative spatially filtered wavefront sensor, the system will be optimized to control scattered light over a large radius and suppress artifacts caused by static errors. We predict that it will achieve contrast levels of 10{sup 7}-10{sup 8} at angular separations of 0.2-0.8 inches around a large sample of stars (R<7-10), sufficient to detect Jupiter-like planets through their near-IR emission over a wide range of ages and masses. We are constructing a high-contrast AO testbed to verify key concepts of our system, and present preliminary results here, showing an RMS wavefront error of <1.3 nm with a flat mirror.

  17. The Giant Magellan Telescope adaptive optics program

    Science.gov (United States)

    Bouchez, Antonin H.; Acton, D. Scott; Agapito, Guido; Arcidiacono, Carmelo; Bennet, Francis; Biliotti, Valdemaro; Bonaglia, Marco; Briguglio, Runa; Brusa-Zappellini, Guido; Busoni, Lorenzo; Carbonaro, Luca; Codona, Johanan L.; Conan, Rodolphe; Connors, Thomas; Durney, Oliver; Espeland, Brady; Esposito, Simone; Fini, Luca; Gardhouse, Rusty; Gauron, Thomas M.; Hart, Michael; Hinz, Philip M.; Kanneganti, Srikrishna; Kibblewhite, Edward J.; Knox, Russell P.; McLeod, Brian A.; McMahon, Thomas; Montoya, Manny; Norton, Timothy J.; Ordway, Mark P.; d'Orgeville, Celine; Parcell, Simon; Piatrou, Piotr K.; Pinna, Enrico; Price, Ian; Puglisi, Alfio; Quiros-Pacheco, Fernando; Riccardi, Armando; Roll, John B.; Trancho, Gelys; Uhlendorf, Kristina; Vaitheeswaran, Vidhya; van Dam, Marcos A.; Weaver, David; Xompero, Marco

    2012-07-01

    The Giant Magellan Telescope adaptive optics system will be an integral part of the telescope, providing laser guide star generation, wavefront sensing, and wavefront correction to most of the currently envisioned instruments. The system will provide three observing modes: Natural Guidestar AO (NGSAO), Laser Tomography AO (LTAO), and Ground Layer AO (GLAO). Every AO observing mode will use the telescope’s segmented adaptive secondary mirror to deliver a corrected beam directly to the instruments. High-order wavefront sensing for the NGSAO and LTAO modes is provided by a set of wavefront sensors replicated for each instrument and fed by visible light reflected off the cryostat window. An infrared natural guidestar wavefront sensor with open-loop AO correction is also required to sense tip-tilt, focus, segment piston, and dynamic calibration errors in the LTAO mode. GLAO mode wavefront sensing is provided by laser guidestars over a ~5 arcminute field of view, and natural guidestars over wider fields. A laser guidestar facility will project 120 W of 589 nm laser light in 6 beacons from the periphery of the primary mirror. An off-axis phasing camera and primary and secondary mirror metrology systems will ensure that the telescope optics remain phased. We describe the system requirements, overall architecture, and innovative solutions found to the challenges presented by high-order AO on a segmented extremely large telescope. Further details may be found in specific papers on each of the observing modes and major subsystems.

  18. The Coming of Age of Adaptive Optics

    Science.gov (United States)

    1995-10-01

    How Ground-Based Astronomers Beat the Atmosphere Adaptive Optics (AO) is the new ``wonder-weapon'' in ground-based astronomy. By means of advanced electro-optical devices at their telescopes, astronomers are now able to ``neutralize'' the image-smearing turbulence of the terrestrial atmosphere (seen by the unaided eye as the twinkling of stars) so that much sharper images can be obtained than before. In practice, this is done with computer-controlled, flexible mirrors which refocus the blurred images up to 100 times per second, i.e. at a rate that is faster than the changes in the atmospheric turbulence. This means that finer details in astronomical objects can be studied and also - because of the improved concentration of light in the telescope's focal plane - that fainter objects can be observed. At the moment, Adaptive Optics work best in the infrared part of spectrum, but at some later time it may also significantly improve observations at the shorter wavelengths of visible light. The many-sided aspects of this new technology and its impact on astronomical instrumentation was the subject of a recent AO conference [1] with over 150 participants from about 30 countries, presenting a total of more than 100 papers. The Introduction of AO Techniques into Astronomy The scope of this meeting was the design, fabrication and testing of AO systems, characterisation of the sources of atmospheric disturbance, modelling of compensation systems, individual components, astronomical AO results, non-astronomical applications, laser guide star systems, non-linear optical phase conjugation, performance evaluation, and other areas of this wide and complex field, in which front-line science and high technology come together in a new and powerful symbiosis. One of the specific goals of the meeting was to develop contacts between AO scientists and engineers in the western world and their colleagues in Russia and Asia. For the first time at a conference of this type, nine Russian

  19. Optically sensitive Medipix2 detector for adaptive optics wavefront sensing

    CERN Document Server

    Vallerga, John; Tremsina, Anton; Siegmund, Oswald; Mikulec, Bettina; Clark, Allan G; CERN. Geneva

    2005-01-01

    A new hybrid optical detector is described that has many of the attributes desired for the next generation adaptive optics (AO) wavefront sensors. The detector consists of a proximity focused microchannel plate (MCP) read out by multi-pixel application specific integrated circuit (ASIC) chips developed at CERN ("Medipix2") with individual pixels that amplify, discriminate and count input events. The detector has 256 x 256 pixels, zero readout noise (photon counting), can be read out at 1 kHz frame rates and is abutable on 3 sides. The Medipix2 readout chips can be electronically shuttered down to a temporal window of a few microseconds with an accuracy of 10 ns. When used in a Shack-Hartmann style wavefront sensor, a detector with 4 Medipix chips should be able to centroid approximately 5000 spots using 7 x 7 pixel sub-apertures resulting in very linear, off-null error correction terms. The quantum efficiency depends on the optical photocathode chosen for the bandpass of interest.

  20. An adaptive optics imaging system designed for clinical use

    Science.gov (United States)

    Zhang, Jie; Yang, Qiang; Saito, Kenichi; Nozato, Koji; Williams, David R.; Rossi, Ethan A.

    2015-01-01

    Here we demonstrate a new imaging system that addresses several major problems limiting the clinical utility of conventional adaptive optics scanning light ophthalmoscopy (AOSLO), including its small field of view (FOV), reliance on patient fixation for targeting imaging, and substantial post-processing time. We previously showed an efficient image based eye tracking method for real-time optical stabilization and image registration in AOSLO. However, in patients with poor fixation, eye motion causes the FOV to drift substantially, causing this approach to fail. We solve that problem here by tracking eye motion at multiple spatial scales simultaneously by optically and electronically integrating a wide FOV SLO (WFSLO) with an AOSLO. This multi-scale approach, implemented with fast tip/tilt mirrors, has a large stabilization range of ± 5.6°. Our method consists of three stages implemented in parallel: 1) coarse optical stabilization driven by a WFSLO image, 2) fine optical stabilization driven by an AOSLO image, and 3) sub-pixel digital registration of the AOSLO image. We evaluated system performance in normal eyes and diseased eyes with poor fixation. Residual image motion with incremental compensation after each stage was: 1) ~2–3 arc minutes, (arcmin) 2) ~0.5–0.8 arcmin and, 3) ~0.05–0.07 arcmin, for normal eyes. Performance in eyes with poor fixation was: 1) ~3–5 arcmin, 2) ~0.7–1.1 arcmin and 3) ~0.07–0.14 arcmin. We demonstrate that this system is capable of reducing image motion by a factor of ~400, on average. This new optical design provides additional benefits for clinical imaging, including a steering subsystem for AOSLO that can be guided by the WFSLO to target specific regions of interest such as retinal pathology and real-time averaging of registered images to eliminate image post-processing. PMID:26114033

  1. An adaptive optics imaging system designed for clinical use.

    Science.gov (United States)

    Zhang, Jie; Yang, Qiang; Saito, Kenichi; Nozato, Koji; Williams, David R; Rossi, Ethan A

    2015-06-01

    Here we demonstrate a new imaging system that addresses several major problems limiting the clinical utility of conventional adaptive optics scanning light ophthalmoscopy (AOSLO), including its small field of view (FOV), reliance on patient fixation for targeting imaging, and substantial post-processing time. We previously showed an efficient image based eye tracking method for real-time optical stabilization and image registration in AOSLO. However, in patients with poor fixation, eye motion causes the FOV to drift substantially, causing this approach to fail. We solve that problem here by tracking eye motion at multiple spatial scales simultaneously by optically and electronically integrating a wide FOV SLO (WFSLO) with an AOSLO. This multi-scale approach, implemented with fast tip/tilt mirrors, has a large stabilization range of ± 5.6°. Our method consists of three stages implemented in parallel: 1) coarse optical stabilization driven by a WFSLO image, 2) fine optical stabilization driven by an AOSLO image, and 3) sub-pixel digital registration of the AOSLO image. We evaluated system performance in normal eyes and diseased eyes with poor fixation. Residual image motion with incremental compensation after each stage was: 1) ~2-3 arc minutes, (arcmin) 2) ~0.5-0.8 arcmin and, 3) ~0.05-0.07 arcmin, for normal eyes. Performance in eyes with poor fixation was: 1) ~3-5 arcmin, 2) ~0.7-1.1 arcmin and 3) ~0.07-0.14 arcmin. We demonstrate that this system is capable of reducing image motion by a factor of ~400, on average. This new optical design provides additional benefits for clinical imaging, including a steering subsystem for AOSLO that can be guided by the WFSLO to target specific regions of interest such as retinal pathology and real-time averaging of registered images to eliminate image post-processing.

  2. Receding-horizon adaptive contyrol of aero-optical wavefronts

    NARCIS (Netherlands)

    Tesch, J.; Gibson, S.; Verhaegen, M.

    2013-01-01

    A new method for adaptive prediction and correction of wavefront errors in adaptive optics (AO) is introduced. The new method is based on receding-horizon control design and an adaptive lattice filter. Experimental results presented illustrate the capability of the new adaptive controller to predict

  3. Adaptive interferometric null testing for unknown freeform optics metrology.

    Science.gov (United States)

    Huang, Lei; Choi, Heejoo; Zhao, Wenchuan; Graves, Logan R; Kim, Dae Wook

    2016-12-01

    We report an adaptive interferometric null testing method for overcoming the dynamic range limitations of conventional null testing approaches during unknown freeform optics metrology or optics manufacturing processes that require not-yet-completed surface measurements to guide the next fabrication process. In the presented adaptive method, a deformable mirror functions as an adaptable null component for an unknown optical surface. The optimal deformable mirror's shape is determined by the stochastic parallel gradient descent algorithm and controlled by a deflectometry system. An adaptive interferometric null testing setup was constructed, and its metrology data successfully demonstrated superb adaptive capability in measuring an unknown surface.

  4. Phase retrieval techniques for adaptive optics

    Energy Technology Data Exchange (ETDEWEB)

    Carrano, C. J., LLNL

    1998-03-01

    We have developed and tested a method for minimizing static aberrations in adaptive optics systems. In order to correct the static phase aberrations, we need to measure the aberrations through the entire system. We have employed various phase retrieval algorithms to detect these aberrations. We have performed simulations of our experimental setup demonstrating that phase retrieval can improve the static aberrations to below the 20 nm rms level, with the limiting factor being local turbulence in the A0 system. Experimentally thus far, we have improved the static aberrations down to the 50 nm level, with the limiting factor being the ability to adjust the deformable mirror. This should be improved with better control algorithms now being implemented.

  5. Application of optical scanning for measurements of castings and cores

    Directory of Open Access Journals (Sweden)

    M. Wieczorowski

    2010-01-01

    Full Text Available In the paper application of non destructive method for dimensional control of elements in initial phase of car manufacturing, at Volks-wagen Poznań foundry was presented. VW foundry in Poznań is responsible of series production of chill and dies castings made of light alloys using contemporary technologies. Castings have a complex shape: they are die castings of housings for steering columns and gravity chill castings of cylinder heads, for which cores are manufactured using both hot box and cold box method. Manufacturing capabilities of VW foundry in Poznań reach 26.000 tons of aluminum castings per year. Optical system ATOS at Volkswagen Poznań foundry is used to digitize object and determination of all dimensions and shapes of inspected object. This technology is applied in car industry, reverse engineering, quality analysis and control and to solve many similar tasks. System is based on triangulation: sensor head projects different fringes patterns onto a measured object while scanner observes their trajectories using two cameras. Basing on optical transform equations a processing unit automatically and with a great accuracy calculates 3D coordinates for every pixel of camera. Depending on camera reso-lution as an effect of such a scan we obtain a cloud of up to 4 million points for every single measurement. In the paper examples of di-mensional analysis regarding castings and cores were presented.

  6. Vision science and adaptive optics, the state of the field.

    Science.gov (United States)

    Marcos, Susana; Werner, John S; Burns, Stephen A; Merigan, William H; Artal, Pablo; Atchison, David A; Hampson, Karen M; Legras, Richard; Lundstrom, Linda; Yoon, Geungyoung; Carroll, Joseph; Choi, Stacey S; Doble, Nathan; Dubis, Adam M; Dubra, Alfredo; Elsner, Ann; Jonnal, Ravi; Miller, Donald T; Paques, Michel; Smithson, Hannah E; Young, Laura K; Zhang, Yuhua; Campbell, Melanie; Hunter, Jennifer; Metha, Andrew; Palczewska, Grazyna; Schallek, Jesse; Sincich, Lawrence C

    2017-03-01

    Adaptive optics is a relatively new field, yet it is spreading rapidly and allows new questions to be asked about how the visual system is organized. The editors of this feature issue have posed a series of question to scientists involved in using adaptive optics in vision science. The questions are focused on three main areas. In the first we investigate the use of adaptive optics for psychophysical measurements of visual system function and for improving the optics of the eye. In the second, we look at the applications and impact of adaptive optics on retinal imaging and its promise for basic and applied research. In the third, we explore how adaptive optics is being used to improve our understanding of the neurophysiology of the visual system. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Optically tracked, single-coil, scanning magnetic induction tomography

    Science.gov (United States)

    Feldkamp, Joe R.; Quirk, Stephen

    2017-03-01

    Recent work has shown the feasibility of single-coil, magnetic induction tomography, for visualizing a 3D distribution of electrical conductivity in portions of the human body. Loss is measured in a single, planar coil consisting of concentric circular loops while the coil is relocated to various non-redundant positions and orientations in the vicinity of the target. These loss values, together with measured coil position and orientation, are processed by a quantitative mapping equation that enables reconstruction of an electrical conductivity image. Up until now, the position of the coil had to be established by a template, which required assignment of locations for the coil to visit without necessarily giving any prior consideration to target geometry. We have now added optical tracking to our existing single-coil device so that position and orientation are tracked automatically, allowing collection of coil loss data at arbitrary positions or orientations as needed. Optical tracking is accomplished via a set of IR reflective spheres mounted on the same enclosure that supports the coil. Position for a select sphere within the set, together with the four quaternions specifying optical body orientation, is fed to a laptop at the same time coil loss data is streamed to the same laptop via Bluetooth. The coil center can be tracked with sub-millimeter accuracy while orientation angle is known to a fraction of a degree. This work illustrates the use of single-coil MIT in full, position-orientation-tracked scan mode while imaging laboratory phantoms. Phantoms are based upon simple materials having biologic conductivity (values for the various features within the image.

  8. Acute Solar Retinopathy Imaged With Adaptive Optics, Optical Coherence Tomography Angiography, and En Face Optical Coherence Tomography.

    Science.gov (United States)

    Wu, Chris Y; Jansen, Michael E; Andrade, Jorge; Chui, Toco Y P; Do, Anna T; Rosen, Richard B; Deobhakta, Avnish

    2018-01-01

    Solar retinopathy is a rare form of retinal injury that occurs after direct sungazing. To enhance understanding of the structural changes that occur in solar retinopathy by obtaining high-resolution in vivo en face images. Case report of a young adult woman who presented to the New York Eye and Ear Infirmary with symptoms of acute solar retinopathy after viewing the solar eclipse on August 21, 2017. Results of comprehensive ophthalmic examination and images obtained by fundus photography, microperimetry, spectral-domain optical coherence tomography (OCT), adaptive optics scanning light ophthalmoscopy, OCT angiography, and en face OCT. The patient was examined after viewing the solar eclipse. Visual acuity was 20/20 OD and 20/25 OS. The patient was left-eye dominant. Spectral-domain OCT images were consistent with mild and severe acute solar retinopathy in the right and left eye, respectively. Microperimetry was normal in the right eye but showed paracentral decreased retinal sensitivity in the left eye with a central absolute scotoma. Adaptive optics images of the right eye showed a small region of nonwaveguiding photoreceptors, while images of the left eye showed a large area of abnormal and nonwaveguiding photoreceptors. Optical coherence tomography angiography images were normal in both eyes. En face OCT images of the right eye showed a small circular hyperreflective area, with central hyporeflectivity in the outer retina of the right eye. The left eye showed a hyperreflective lesion that intensified in area from inner to middle retina and became mostly hyporeflective in the outer retina. The shape of the lesion on adaptive optics and en face OCT images of the left eye corresponded to the shape of the scotoma drawn by the patient on Amsler grid. Acute solar retinopathy can present with foveal cone photoreceptor mosaic disturbances on adaptive optics scanning light ophthalmoscopy imaging. Corresponding reflectivity changes can be seen on en face OCT, especially

  9. Proposed Multiconjugate Adaptive Optics Experiment at Lick Observatory

    Energy Technology Data Exchange (ETDEWEB)

    Bauman, B J; Gavel, D T; Flath, L M; Hurd, R L; Max, C E; Olivier, S S

    2001-08-15

    While the theory behind design of multiconjugate adaptive optics (MCAO) systems is growing, there is still a paucity of experience building and testing such instruments. We propose using the Lick adaptive optics (AO) system as a basis for demonstrating the feasibility/workability of MCAO systems, testing underlying assumptions, and experimenting with different approaches to solving MCAO system issues.

  10. Microsphere-based super-resolution scanning optical microscope.

    Science.gov (United States)

    Huszka, Gergely; Yang, Hui; Gijs, Martin A M

    2017-06-26

    High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with super-resolution, i.e. with a resolution beyond the classical diffraction limit. However, when imaging nanostructures on a substrate, the propagation distance of a light wave in the dielectric medium in between the substrate and the microsphere must be small enough to reveal the sample's nanometric features. Therefore, only the central part of an image obtained through a microsphere shows super-resolution details, which are typically ∼100 nm using white light (peak at λ = 600 nm). We have performed finite element simulations of the role of this critical distance in the super-resolution effect. Super-resolution imaging of a sample placed beneath the microsphere is only possible within a very restricted central area of ∼10 μm2, where the separation distance between the substrate and the microsphere surface is very small (∼1 μm). To generate super-resolution images over larger areas of the sample, we have fixed a microsphere on a frame attached to the microscope objective, which is automatically scanned over the sample in a step-by-step fashion. This generates a set of image tiles, which are subsequently stitched into a single super-resolution image (with resolution of λ/4-λ/5) of a sample area of up to ∼104 μm2. Scanning a standard optical microscope objective with microsphere therefore enables super-resolution microscopy over the complete field-of-view of the objective.

  11. Simulation of DKIST solar adaptive optics system

    Science.gov (United States)

    Marino, Jose; Carlisle, Elizabeth; Schmidt, Dirk

    2016-07-01

    Solar adaptive optics (AO) simulations are a valuable tool to guide the design and optimization process of current and future solar AO and multi-conjugate AO (MCAO) systems. Solar AO and MCAO systems rely on extended object cross-correlating Shack-Hartmann wavefront sensors to measure the wavefront. Accurate solar AO simulations require computationally intensive operations, which have until recently presented a prohibitive computational cost. We present an update on the status of a solar AO and MCAO simulation tool being developed at the National Solar Observatory. The simulation tool is a multi-threaded application written in the C++ language that takes advantage of current large multi-core CPU computer systems and fast ethernet connections to provide accurate full simulation of solar AO and MCAO systems. It interfaces with KAOS, a state of the art solar AO control software developed by the Kiepenheuer-Institut fuer Sonnenphysik, that provides reliable AO control. We report on the latest results produced by the solar AO simulation tool.

  12. Angstrom-range optical path-length measurement with a high-speed scanning heterodyne optical interferometer.

    Science.gov (United States)

    Riza, Nabeel A; Arain, Muzammil A

    2003-05-01

    A highly accurate method of optical path-length measurement is introduced by use of a scanning heterodyne optical interferometer with no moving parts. The instrument has demonstrated the potential to measure optical path length at angstrom resolution over continuous thickness in the micrometer range. This optical path length can be used to calculate the thickness of any material if the refractive index is known or to measure the refractive index of the material if the thickness is known. The instrument uses a single acousto-optic device in an in-line ultra-stable reflective geometry to implement rapid scanning in the microsecond domain for thickness measurements of the test medium.

  13. Adaptive optics ophthalmologic systems using dual deformable mirrors

    Energy Technology Data Exchange (ETDEWEB)

    Jones, S; Olivier, S; Chen, D; Sadda, S; Joeres, S; Zawadzki, R; Werner, J S; Miller, D

    2007-02-01

    Adaptive Optics (AO) have been increasingly combined with a variety of ophthalmic instruments over the last decade to provide cellular-level, in-vivo images of the eye. The use of MEMS deformable mirrors in these instruments has recently been demonstrated to reduce system size and cost while improving performance. However, currently available MEMS mirrors lack the required range of motion for correcting large ocular aberrations, such as defocus and astigmatism. In order to address this problem, we have developed an AO system architecture that uses two deformable mirrors, in a woofer/tweeter arrangement, with a bimorph mirror as the woofer and a MEMS mirror as the tweeter. This setup provides several advantages, including extended aberration correction range, due to the large stroke of the bimorph mirror, high order aberration correction using the MEMS mirror, and additionally, the ability to ''focus'' through the retina. This AO system architecture is currently being used in four instruments, including an Optical Coherence Tomography (OCT) system and a retinal flood-illuminated imaging system at the UC Davis Medical Center, a Scanning Laser Ophthalmoscope (SLO) at the Doheny Eye Institute, and an OCT system at Indiana University. The design, operation and evaluation of this type of AO system architecture will be presented.

  14. U-10Mo Sample Preparation and Examination using Optical and Scanning Electron Microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Prabhakaran, Ramprashad [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Joshi, Vineet V. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Rhodes, Mark A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Schemer-Kohrn, Alan L. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Guzman, Anthony D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lavender, Curt A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-03-30

    The purpose of this document is to provide guidelines to prepare specimens of uranium alloyed with 10 weight percent molybdenum (U-10Mo) for optical metallography and scanning electron microscopy. This document also provides instructions to set up an optical microscope and a scanning electron microscope to analyze U-10Mo specimens and to obtain the required information.

  15. U-10Mo Sample Preparation and Examination using Optical and Scanning Electron Microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Prabhakaran, Ramprashad [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Joshi, Vineet V. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Rhodes, Mark A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Schemer-Kohrn, Alan L. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Guzman, Anthony D. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lavender, Curt A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-10-01

    The purpose of this document is to provide guidelines to prepare specimens of uranium alloyed with 10 weight percent molybdenum (U-10Mo) for optical metallography and scanning electron microscopy. This document also provides instructions to set up an optical microscope and a scanning electron microscope to analyze U-10Mo specimens and to obtain the required information.

  16. Linear quadratic Gaussian control for adaptive optics and multiconjugate adaptive optics: experimental and numerical analysis.

    Science.gov (United States)

    Petit, Cyril; Conan, Jean-Marc; Kulcsár, Caroline; Raynaud, Henri-François

    2009-06-01

    We present a comprehensive analysis of the linear quadratic Gaussian control approach applied to adaptive optics (AO) and multiconjugated AO (MCAO) based on numerical and experimental validations. The structure of the control law is presented and its main properties discussed. We then propose an extended experimental validation of this control law in AO and a simplified MCAO configuration. Performance is compared with end-to-end numerical simulations. Sensitivity of the performance regarding tuning parameters is tested. Finally, extension to full MCAO and laser tomographic AO (LTAO) through numerical simulation is presented and analyzed.

  17. ComPoScan: Adaptive Scanning for Efficient Concurrent Communications and Positioning with 802.11

    DEFF Research Database (Denmark)

    King, Thomas; Kjærgaard, Mikkel Baun

    2008-01-01

    Using 802.11 concurrently for communications and positioning is problematic, especially if location-based services (e.g., indoor navigation) are concurrently executed with real-time applications (e.g., VoIP, video conferencing). Periodical scanning for measuring the signal strength interrupts...

  18. Adaptive optics retinal imaging in the living mouse eye

    Science.gov (United States)

    Geng, Ying; Dubra, Alfredo; Yin, Lu; Merigan, William H.; Sharma, Robin; Libby, Richard T.; Williams, David R.

    2012-01-01

    Correction of the eye’s monochromatic aberrations using adaptive optics (AO) can improve the resolution of in vivo mouse retinal images [Biss et al., Opt. Lett. 32(6), 659 (2007) and Alt et al., Proc. SPIE 7550, 755019 (2010)], but previous attempts have been limited by poor spot quality in the Shack-Hartmann wavefront sensor (SHWS). Recent advances in mouse eye wavefront sensing using an adjustable focus beacon with an annular beam profile have improved the wavefront sensor spot quality [Geng et al., Biomed. Opt. Express 2(4), 717 (2011)], and we have incorporated them into a fluorescence adaptive optics scanning laser ophthalmoscope (AOSLO). The performance of the instrument was tested on the living mouse eye, and images of multiple retinal structures, including the photoreceptor mosaic, nerve fiber bundles, fine capillaries and fluorescently labeled ganglion cells were obtained. The in vivo transverse and axial resolutions of the fluorescence channel of the AOSLO were estimated from the full width half maximum (FWHM) of the line and point spread functions (LSF and PSF), and were found to be better than 0.79 μm ± 0.03 μm (STD)(45% wider than the diffraction limit) and 10.8 μm ± 0.7 μm (STD)(two times the diffraction limit), respectively. The axial positional accuracy was estimated to be 0.36 μm. This resolution and positional accuracy has allowed us to classify many ganglion cell types, such as bistratified ganglion cells, in vivo. PMID:22574260

  19. Computer-Controlled 3D Laser Scanning Microscope Based On Optical Disk Technology.

    Science.gov (United States)

    Schweizer, P.; Neveux, L.; Chiaramello, M.; Monteil, P.; Ostrowsky, D. B...

    1987-08-01

    We describe RASCALS* (RAster SCAn Laser System) a 2D and 3D scanning laser microscope and outline it's performance. This system, based on optical disk technology and a PC compatible computer offers an interesting cost/performance ratio compared to existing laser scanning microscopes.

  20. Scanning optical near-field resolution analyzed in terms of communication modes.

    Science.gov (United States)

    Martinsson, Per; Lajunen, Hanna; Friberg, Ari T

    2006-11-13

    We present an analysis of scanning near-field optical microscopy in terms of the so-called communication modes using scalar wave theory. We show that the number of connected modes increases when the scanning distance is decreased, but the number of modes decreases when the size of the scanning aperture is decreased. In the limit of small detector aperture the best-connected mode reduces effectively to the Green function, evaluated at the center of the scanning aperture. We also suggest that the resolution of a scanning optical near-field imaging system is essentially given by the width of the lowest-order communication mode.

  1. Adaptive optical microspectrometers and spectra-selective sensing

    Science.gov (United States)

    Bhalotra, Sameer R.

    The demand for miniaturized optical spectrometer systems is steadily increasing, for applications such as biochemical material identification, atmosphere monitoring, and military target tracking. Unfortunately, sensing front-ends typically generate a burdensome amount of data that must be processed by large computing systems; the required processing back-ends often limit the compactness and efficiency of complete systems. Ideally, we would design new devices that enable easy implementation of data filtering in a miniaturized front-end, with the capability to adapt to a wide range of sensing tasks. Here we present a new micro spectrometer and compatible information processing system, for applications requiring a flexible, portable, complete sensing system. The standing-wave spectrometer enjoys the advantages of a traditional Fourier transform spectrometer, including spectral multiplexing and simple interferogram output; however it has a compact, linear optical design requiring only two components, a partially transmitting photodetector and a movable mirror. We discuss development of a low power, continuously scanning, miniature Si mirror-actuator, and Si and GaAs photodiodes with thin active regions, for visible and near-infrared devices as small as 17 x 13 x 1 mm. We demonstrate spectral resolution of 100 cm-1 (4 run at lambda = 633 nm), and the unique ability to adapt resolution in real time to optimize signal-to-noise ratio. For spectral data processing, we present a new time-domain filtering concept that minimizes computing requirements. For discrimination among a set of known spectra, we directly use the interferograms generated by a Fourier transform spectrometer to calculate inner products in the time domain. Our method efficiently uses prior knowledge of the known spectra to relax data handling requirements, typically by factors of 10--100, enabling real-time spectra-selective imaging. We can also directly identify optical source types by their spectral

  2. CCD-based optical CT scanning of highly attenuating phantoms

    Energy Technology Data Exchange (ETDEWEB)

    Al-Nowais, Shamsa [Department of Physics, University of Surrey, Guildford (United Kingdom); Doran, Simon J [CRUK Clinical MR Research Group, Institute of Cancer Research, Sutton (United Kingdom)], E-mail: Simon.Doran@icr.ac.uk

    2009-05-01

    The introduction of optical computed tomography (optical-CT) offers economic and easy to use 3-D optical readout for gel dosimeters. However, previous authors have noted some challenges regarding the accuracy of such imaging techniques at high values of optical density. In this paper, we take a closer look at the 'cupping' artefact evident in both light-scattering polymer systems and highly light absorbing phantoms using our CCD-based optical scanner. In addition, a technique is implemented whereby the maximum measurable optical absorbance is extended to correct for any errors that may have occurred in the estimated value of the dark current or ambient light reaching the detector. The results indicate that for absorbance values up to 2.0, the optical scanner results have good accuracy, whereas this is not the case at high absorbance values for reasons yet to be explained.

  3. Astronomy Applications of Adaptive Optics at Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Bauman, B J; Gavel, D T

    2003-04-23

    Astronomical applications of adaptive optics at Lawrence Livermore National Laboratory (LLNL) has a history that extends from 1984. The program started with the Lick Observatory Adaptive Optics system and has progressed through the years to lever-larger telescopes: Keck, and now the proposed CELT (California Extremely Large Telescope) 30m telescope. LLNL AO continues to be at the forefront of AO development and science.

  4. A Status Report on the Thirty Meter Telescope Adaptive Optics ...

    Indian Academy of Sciences (India)

    Adaptive Optics Program. B. L. Ellerbroek. TMT Observatory Corporation, 1111 S. Arroyo Pkwy., Ste. 200, Pasadena, CA 91107, USA. e-mail: brente@caltech.edu. Received 15 March 2013; accepted 24 May 2013. Abstract. We provide an update on the recent development of the adap- tive optics (AO) systems for the Thirty ...

  5. Adaptive Forward Error Correction for Energy Efficient Optical Transport Networks

    DEFF Research Database (Denmark)

    Rasmussen, Anders; Ruepp, Sarah Renée; Berger, Michael Stübert

    2013-01-01

    In this paper we propose a novel scheme for on the fly code rate adjustment for forward error correcting (FEC) codes on optical links. The proposed scheme makes it possible to adjust the code rate independently for each optical frame. This allows for seamless rate adaption based on the link state...

  6. ADAPTIVE OPTICS IMAGING OF FOVEAL SPARING IN GEOGRAPHIC ATROPHY SECONDARY TO AGE-RELATED MACULAR DEGENERATION.

    Science.gov (United States)

    Querques, Giuseppe; Kamami-Levy, Cynthia; Georges, Anouk; Pedinielli, Alexandre; Capuano, Vittorio; Blanco-Garavito, Rocio; Poulon, Fanny; Souied, Eric H

    2016-02-01

    To describe adaptive optics (AO) imaging of foveal sparing in geographic atrophy (GA) secondary to age-related macular degeneration. Flood-illumination AO infrared (IR) fundus images were obtained in four consecutive patients with GA using an AO retinal camera (rtx1; Imagine Eyes). Adaptive optics IR images were overlaid with confocal scanning laser ophthalmoscope near-IR autofluorescence images to allow direct correlation of en face AO features with areas of foveal sparing. Adaptive optics appearance of GA and foveal sparing, preservation of functional photoreceptors, and cone densities in areas of foveal sparing were investigated. In 5 eyes of 4 patients (all female; mean age 74.2 ± 11.9 years), a total of 5 images, sized 4° × 4°, of foveal sparing visualized on confocal scanning laser ophthalmoscope near-IR autofluorescence were investigated by AO imaging. En face AO images revealed GA as regions of inhomogeneous hyperreflectivity with irregularly dispersed hyporeflective clumps. By direct comparison with adjacent regions of GA, foveal sparing appeared as well-demarcated areas of reduced reflectivity with less hyporeflective clumps (mean 14.2 vs. 3.2; P = 0.03). Of note, in these areas, en face AO IR images revealed cone photoreceptors as hyperreflective dots over the background reflectivity (mean cone density 3,271 ± 1,109 cones per square millimeter). Microperimetry demonstrated residual function in areas of foveal sparing detected by confocal scanning laser ophthalmoscope near-IR autofluorescence. Adaptive optics allows the appreciation of differences in reflectivity between regions of GA and foveal sparing. Preservation of functional cone photoreceptors was demonstrated on en face AO IR images in areas of foveal sparing detected by confocal scanning laser ophthalmoscope near-IR autofluorescence.

  7. Problems of Aero-optics and Adaptive Optical Systems: Analytical Review

    Directory of Open Access Journals (Sweden)

    Yu. I. Shanin

    2017-01-01

    Full Text Available The analytical review gives the basic concepts of the aero-optics problem arising from the radiation propagation in the region of the boundary layers of a laser installation carrier aircraft. Estimates the radiation wave front distortions at its propagation in the near and far field. Presents main calculation approaches and methods to solve the gas-dynamic and optical problems in propagating laser radiation. Conducts a detailed analysis of the flows and their generating optical aberrations introduced by the aircraft turret (a projection platform of the on-board laser. Considers the effect of various factors (shock wave, difference in wall and flow temperatures on the flow pattern and the optical aberrations. Provides research data on the aero-optics obtained in the flying laboratory directly while in flight. Briefly considers the experimental research methods, diagnostic equipment, and synthesis of results while studying the aero-optics problem. Discusses some methods for mitigating the aerodynamic effects on the light propagation under flight conditions. Presents data about the passive, active, and hybrid effects on the flow in the boundary layers in order to reduce aberrations through improving the flow aerodynamics.The paper considers operation of adaptive optical systems under conditions of aero-optical distortions. Presents the study results concerning the reduction of the aero-optics effect on the characteristics of radiation in far field. Gives some research results regarding the effect on the efficiency of the adaptive system of a laser beam jitter and a time delay in the feedback signal transmission, which occur under application conditions. Provides data on adaptive correction of aero-optical wave fronts of radiation. Considers some application aspects in control systems of the on-board adaptive optics of adaptive filtration as a way to improve the efficiency of adaptive optical systems. The project in mind is to use obtained results

  8. MPEG-2 video coding with an adaptive selection of scanning path and picture structure

    Science.gov (United States)

    Zhou, Minhua; De Lameillieure, Jan L.; Schaefer, Ralf

    1996-09-01

    In the MPEG-2 video coding an interlaced frame can be encoded as either a frame-picture or two field-pictures. The selection of picture structure (frame/field) has a strong impact on picture quality. In order to achieve the best possible picture quality, an adaptive scheme is proposed in this paper to select the optimal picture structure on a frame by frame basis. The selection of picture structure is performed in connection with that of the optimal scanning path. First, the scanning path (zig-zag scan/alternate scan) is chosen based on a post-analysis of DCT-coefficients. Secondly, the optimal picture structure is selected for the next frame according to the chosen scanning path, i.e. a zig-zag scan corresponds to frame picture structure, while an alternate scan corresponds to field picture structure. Furthermore, the TM5 buffer control algorithm is extended to support the coding with adaptive frame/field picture structure. Finally, simulation results verify the adaptive scheme proposed in this paper.

  9. Adaptive Volterra equalizer for optical OFDM modem

    Science.gov (United States)

    Mhatli, Sofien; Nsiri, Bechir; Jarajreh, Mutsam A.; Channoufi, Malek; Attia, Rabah

    2015-01-01

    This paper addresses OFDM (orthogonal frequency division multiplexing) transmission over optical links with high spectral efficiency, i.e. by using high-order QAM-modulation schemes as a mapping method prior to the OFDM multicarrier representation. Here we address especially coherent optical OFDM modem in long distance which is affected by a nonlinear distortion caused by fiber nonlinearity as a major performance-limiting factor in advanced optical communication systems. We proposed a nonlinear electrical equalization scheme based on the Volterra model. Compared with other popular linear compensation technique such as the LMS (least Mean Square) and RLS (Recursive Least square), simulation results are presented to demonstrate the capability of a Volterra model based electrical equalizer used in a coherent optical orthogonal frequency division multiplexing system. It is shown that the Volterra model based equalizer can significantly reduce nonlinear distortion.

  10. Amplitude variations on the Extreme Adaptive Optics testbed

    Energy Technology Data Exchange (ETDEWEB)

    Evans, J; Thomas, S; Dillon, D; Gavel, D; Phillion, D; Macintosh, B

    2007-08-14

    High-contrast adaptive optics systems, such as those needed to image extrasolar planets, are known to require excellent wavefront control and diffraction suppression. At the Laboratory for Adaptive Optics on the Extreme Adaptive Optics testbed, we have already demonstrated wavefront control of better than 1 nm rms within controllable spatial frequencies. Corresponding contrast measurements, however, are limited by amplitude variations, including those introduced by the micro-electrical-mechanical-systems (MEMS) deformable mirror. Results from experimental measurements and wave optic simulations of amplitude variations on the ExAO testbed are presented. We find systematic intensity variations of about 2% rms, and intensity variations with the MEMS to be 6%. Some errors are introduced by phase and amplitude mixing because the MEMS is not conjugate to the pupil, but independent measurements of MEMS reflectivity suggest that some error is introduced by small non-uniformities in the reflectivity.

  11. DYNAMISM OF DOT SUBRETINAL DRUSENOID DEPOSITS IN AGE-RELATED MACULAR DEGENERATION DEMONSTRATED WITH ADAPTIVE OPTICS IMAGING.

    Science.gov (United States)

    Zhang, Yuhua; Wang, Xiaolin; Godara, Pooja; Zhang, Tianjiao; Clark, Mark E; Witherspoon, C Douglas; Spaide, Richard F; Owsley, Cynthia; Curcio, Christine A

    2018-01-01

    To investigate the natural history of dot subretinal drusenoid deposits (SDD) in age-related macular degeneration, using high-resolution adaptive optics scanning laser ophthalmoscopy. Six eyes of four patients with intermediate age-related macular degeneration were studied at baseline and 1 year later. Individual dot SDD within the central 30° retina were examined with adaptive optics scanning laser ophthalmoscopy and optical coherence tomography. A total of 269 solitary SDD were identified at baseline. Over 12.25 ± 1.18 months, all 35 Stage 1 SDD progressed to advanced stages. Eighteen (60%) Stage 2 lesions progressed to Stage 3 and 12 (40%) remained at Stage 2. Of 204 Stage 3 SDD, 12 (6.4%) disappeared and the rest remained. Twelve new SDD were identified, including 6 (50%) at Stage 1, 2 (16.7%) at Stage 2, and 4 (33.3%) at Stage 3. The mean percentage of the retina affected by dot SDD, measured by the adaptive optics scanning laser ophthalmoscopy, increased in 5/6 eyes (from 2.31% to 5.08% in the most changed eye) and decreased slightly in 1/6 eye (from 10.67% to 10.54%). Dynamism, the absolute value of the areas affected by new and regressed lesions, ranged from 0.7% to 9.3%. Adaptive optics scanning laser ophthalmoscopy reveals that dot SDD, like drusen, are dynamic.

  12. Performance analysis of a hybrid fingerprint extracted from optical coherence tomography fingertip scans

    CSIR Research Space (South Africa)

    Darlow, Luke N

    2016-06-01

    Full Text Available International Conference on Biometrics (ICB), 13-16 June 2016, Halmstad, Sweden Performance analysis of a hybrid fingerprint extracted from optical coherence tomography fingertip scans Darlow LN Connan J Singh A ABSTRACT: The Hybrid fingerprint is a...

  13. Angular scan optical coherence tomography imaging and metrology of spherical gradient refractive index preforms.

    Science.gov (United States)

    Yao, Jianing; Meemon, Panomsak; Ponting, Michael; Rolland, Jannick P

    2015-03-09

    The fabrication of high-performance spherical gradient refractive index (S-GRIN) optics requires nondestructive metrology techniques to inspect the samples. We have developed an angular-scan, swept-source-based, Fourier-domain optical coherence tomography (OCT) system centered at 1318 nm with 5 mm imaging depth capable of 180° polar scan and 360° azimuthal scan to investigate polymeric S-GRIN preforms. We demonstrate a method that enables simultaneous mapping of the group optical thickness, physical thickness, the radially-averaged group refractive index, and the transmitted wavefront of the S-GRIN preforms. The angular scan OCT imaging and metrology enables direct visualization, molding uniformity characterization, and optical property evaluations of the preforms. The results on two generations of S-GRIN preforms are discussed that showcase the evolution of the manufacturing process in response to the OCT metrology feedback.

  14. Field programmable gate array based reconfigurable scanning probe/optical microscope.

    Science.gov (United States)

    Nowak, Derek B; Lawrence, A J; Dzegede, Zechariah K; Hiester, Justin C; Kim, Cliff; Sánchez, Erik J

    2011-10-01

    The increasing popularity of nanometrology and nanospectroscopy has pushed researchers to develop complex new analytical systems. This paper describes the development of a platform on which to build a microscopy tool that will allow for flexibility of customization to suit research needs. The novelty of the described system lies in its versatility of capabilities. So far, one version of this microscope has allowed for successful near-field and far-field fluorescence imaging with single molecule detection sensitivity. This system is easily adapted for reflection, polarization (Kerr magneto-optical (MO)), Raman, super-resolution techniques, and other novel scanning probe imaging and spectroscopic designs. While collecting a variety of forms of optical images, the system can simultaneously monitor topographic information of a sample with an integrated tuning fork based shear force system. The instrument has the ability to image at room temperature and atmospheric pressure or under liquid. The core of the design is a field programmable gate array (FPGA) data acquisition card and a single, low cost computer to control the microscope with analog control circuitry using off-the-shelf available components. A detailed description of electronics, mechanical requirements, and software algorithms as well as examples of some different forms of the microscope developed so far are discussed.

  15. Fabrication and characterization of probes for combined scanning electrochemical/optical microscopy experiments.

    Science.gov (United States)

    Lee, Youngmi; Bard, Allen J

    2002-08-01

    A technique that combines scanning electrochemical microscopy (SECM) and optical microscopy (OM) was implemented with a new probe tip. The tip for scanning electrochemicaVoptical microscopy (SECM/OM) was constructed by insulating a typical gold-coated near-field scanning optical microscopy tip using electrophoretic anodic paint. Once fabricated, the tip was characterized by steady-state cyclic voltammetry, as well as optical and electrochemical approach experiments. This tip generated a stable steady-state current and well-defined SECM approach curves for both conductive and insulating substrates. Durable tips whose geometry was a ring with < 1 microm as outer ring radius could be consistently fabricated. Simultaneous electrochemical and optical images of an interdigitated array electrode were obtained with a resolution on the micrometer scale, demonstrating good performance of the tip as both an optical and an electrochemical probe for imaging microstructures. The SECM feedback current measurements were successfully employed to determine tip-substrate distances for imaging.

  16. PSD microscopy: a new technique for adaptive local scanning of microscale objects.

    Science.gov (United States)

    Rahimi, Mehdi; Shen, Yantao

    2017-01-01

    A position-sensitive detector/device (PSD) is a sensor that is capable of tracking the location of a laser beam on its surface. PSDs are used in many scientific instruments and technical applications including but not limited to atomic force microscopy, human eye movement monitoring, mirrors or machine tool alignment, vibration analysis, beam position control and so on. This work intends to propose a new application using the PSD. That is a new microscopy system called scanning PSD microscopy. The working mechanism is about putting an object on the surface of the PSD and fast scanning its area with a laser beam. To achieve a high degree of accuracy and precision, a reliable framework was designed using the PSD. In this work, we first tried to improve the PSD reading and its measurement performance. This was done by minimizing the effects of noise, distortion and other disturbing parameters. After achieving a high degree of confidence, the microscopy system can be implemented based on the improved PSD measurement performance. Later to improve the scanning efficiency, we developed an adaptive local scanning system to scan the whole area of the PSD in a short matter of time. It was validated that our comprehensive and adaptive local scanning method can shorten the scanning time in order of hundreds of times in comparison with the traditional raster scanning without losing any important information about the scanned 2D objects. Methods are also introduced to scan very complicated objects with bifurcations and crossings. By incorporating all these methods, the new microscopy system is capable of scanning very complicated objects in the matter of a few seconds with a resolution that is in order of a few micrometers.

  17. Accuracy requirements of optical linear algebra processors in adaptive optics imaging systems

    Science.gov (United States)

    Downie, John D.

    1990-01-01

    A ground-based adaptive optics imaging telescope system attempts to improve image quality by detecting and correcting for atmospherically induced wavefront aberrations. The required control computations during each cycle will take a finite amount of time. Longer time delays result in larger values of residual wavefront error variance since the atmosphere continues to change during that time. Thus an optical processor may be well-suited for this task. This paper presents a study of the accuracy requirements in a general optical processor that will make it competitive with, or superior to, a conventional digital computer for the adaptive optics application. An optimization of the adaptive optics correction algorithm with respect to an optical processor's degree of accuracy is also briefly discussed.

  18. CHARACTERIZING PHOTORECEPTOR CHANGES IN ACUTE POSTERIOR MULTIFOCAL PLACOID PIGMENT EPITHELIOPATHY USING ADAPTIVE OPTICS.

    Science.gov (United States)

    Roberts, Philipp K; Nesper, Peter L; Onishi, Alex C; Skondra, Dimitra; Jampol, Lee M; Fawzi, Amani A

    2018-01-01

    To characterize lesions of acute posterior multifocal placoid pigment epitheliopathy (APMPPE) by multimodal imaging including adaptive optics scanning laser ophthalmoscopy (AOSLO). We included patients with APMPPE at different stages of evolution of the placoid lesions. Color fundus photography, spectral domain optical coherence tomography, infrared reflectance, fundus autofluorescence, and AOSLO images were obtained and registered to correlate microstructural changes. Eight eyes of four patients (two women) were included and analyzed by multimodal imaging. Photoreceptor reflectivity within APMPPE lesions was more heterogeneous than in adjacent healthy areas. Hyperpigmentation on color fundus photography appeared hyperreflective on infrared reflectance and on AOSLO. Irregularity of the interdigitation zone and the photoreceptor inner and outer segment junctions (IS/OS) on spectral domain optical coherence tomography was associated with photoreceptor hyporeflectivity on AOSLO. Interruption of the interdigitation zone or IS/OS was associated with loss of photoreceptor reflectivity on AOSLO. Irregularities in the reflectivity of the photoreceptor mosaic are visible on AOSLO even in inactive APMPPE lesions, where the photoreceptor bands on spectral domain optical coherence tomography have recovered. Adaptive optics scanning laser ophthalmoscopy combined with multimodal imaging has the potential to enhance our understanding of photoreceptor involvement in APMPPE.

  19. The Adaptive Optics Facility: Commissioning Progress and Results

    Science.gov (United States)

    Arsenault, R.; Madec, P.-Y.; Vernet, E.; Hackenberg, W.; La Penna, P.; Paufique, J.; Kuntschner, H.; Pirard, J.-F.; Kolb, J.; Hubin, N.

    2017-06-01

    All the Adaptive Optics Facility (AOF) subsystems are now in Paranal and the project team is working on commissioning activities on Unit Telescope 4 (UT4) of the Very Large Telescope. Excellent progress has been made; the new secondary mirror unit, the Deformable Secondary Mirror (DSM), was installed in October 2016 and UT4 is now operating routinely with the DSM in non-adaptive optics mode. The other modules of the AOF, the Ground Atmospheric Layer Adaptive optiCs for Spectroscopic Imaging (GALACSI), the 4 Laser Guide Star Facility (4LGSF) and the GRound-layer Adaptive optics Assisted by Lasers (GRAAL), have been installed and are being qualified. The coupling with the High Acuity Wide field K-band Imager (HAWK-I) and the Multi Unit Spectroscopic Explorer (MUSE) has been tested and all elements are functional and ready to proceed with their full commissioning. The goal for the AOF is to complete GALACSI wide-field mode technical commissioning by the end of summer 2017 and the GRAAL ground-layer adaptive optics mode by the end of the year.

  20. Very Large Telescope Adaptive Optics Community Days Report on the ESO Workshop

    Science.gov (United States)

    Leibundgut, B.; Kasper, M.; Kuntschner, H.

    2016-12-01

    The future of adaptive optics (AO) instruments at the VLT was discussed during a two-day workshop. Three major directions emerged from these discussions: adaptive optics in the optical; multi-object adaptive optics (MOAO); and extreme adaptive optics (XAO). The science cases for these three options were presented and the discussions are summarised. ESO is now planning to provide detailed science cases for an optical AO system and to prepare upgrade plans for XAO and MOAO.

  1. Extragalactic Fields Optimized for Adaptive Optics

    Science.gov (United States)

    2011-03-01

    Universidade do Porto. Rua"das Estrelas, 4150-762 Porto, Portugal. S Canada-Prance-Hawaii Telescope Corporation, 65-1238 Mamalahoa High- way, Kamuela, HI...poses it suffices to note that MCAO’s purpose is to deliver high image quality over a wider area than conventional adaptive op- tics systems, and it does

  2. Light propagation studies on laser modified waveguides using scanning near-field optical microscopy

    DEFF Research Database (Denmark)

    Borrise, X.; Berini, Abadal Gabriel; Jimenez, D.

    2001-01-01

    microscope (SNOM) has been used. The laser modifications locally changes the optical properties of the waveguide. The change in the effective refractive index is attributed to a TE to TM mode conversion, Thus, the laser modification might be a new way to fabricate optical mode converters.......By means of direct laser writing on Al, a new method to locally modify optical waveguides is proposed. This technique has been applied to silicon nitride waveguides, allowing modifications of the optical propagation along the guide. To study the formed structures, a scanning near-held optical...

  3. Tracking dynamics of photoreceptor disc shedding with adaptive optics-optical coherence tomography

    Science.gov (United States)

    Zhang, Furu; Liu, Zhuolin; Kurokawa, Kazuhiro; Miller, Donald T.

    2017-02-01

    Absorption of light by photoreceptors initiates vision, but also leads to accumulation of toxic photo-oxidative compounds in the photoreceptor outer segment (OS). To prevent this buildup, small packets of OS discs are periodically pruned from the distal end of the OS, a process called disc shedding. Unfortunately dysfunction in any part of the shedding event can lead to photoreceptor and RPE dystrophy, and has been implicated in numerous retinal diseases, including age related macular degeneration and retinitis pigmentosa. While much is known about the complex molecular and signaling pathways that underpin shedding, all of these advancements have occurred in animal models using postmortem eyes. How these translate to the living retina and to humans remain major obstacles. To that end, we have recently discovered the optical signature of cone OS disc shedding in the living human retina, measured noninvasively using optical coherence tomography equipped with adaptive optics in conjunction with post processing methods to track and monitor individual cones in 4D. In this study, we improve on this method in several key areas: increasing image acquisition up to MHz A-scan rates, improving reliability to detect disc shedding events, establishing system precision, and developing cone tracking for use across the entire awake cycle. Thousands of cones were successfully imaged and tracked over the 17 hour period in two healthy subjects. Shedding events were detected in 79.5% and 77.4% of the tracked cones. Similar to previous animal studies, shedding prevalence exhibited a diurnal rhythm. But we were surprised to find that for these two subjects shedding occurred across the entire day with broad, elevated frequency in the morning and decreasing frequency as the day progressed. Consistent with this, traces of the average cone OS length revealed shedding dominated in the morning and afternoon and renewal in the evening.

  4. Optimization-based adaptive optics for optical coherence tomography

    NARCIS (Netherlands)

    Verstraete, H.R.G.W.

    2017-01-01

    Optical coherence tomography (OCT) is a technique for non-invasive imaging based on low coherence interferometry. Its main application is found in ophthalmology, where it is used for 3D in vivo imaging of the cornea and the retina. OCT has evolved over the past decade as one of the most important

  5. Continuous measurement of optical surfaces using a line-scan interferometer with sinusoidal path length modulation.

    Science.gov (United States)

    Knell, Holger; Laubach, Sören; Ehret, Gerd; Lehmann, Peter

    2014-12-01

    We present a fast approach to the continuous measurement of rotational symmetric optical surfaces. This approach is based on a line scanning interferometer with sinusoidal modulation of the optical path length. The specimen is positioned with respect to the sensor and both are moved during measurement by use of a five axes system comprising a high precision rotational table. The calibration of both the line sensor as well as the scanning and positioning system is discussed. As proof of principle of the measurement and stitching concept results of a scan of a rotational symmetric sinusoidal structure and a spherical lens with a moderate slope are shown.

  6. An Evanescent Field Optical Microscope. Scanning probe Microscopy

    NARCIS (Netherlands)

    van Hulst, N.F.; Segerink, Franciscus B.; Bölger, B.; Bölger, B.; Wickramasinghe, H. Kumar

    1991-01-01

    An Evanescent Field Optical Microscope (EFOM) is presented, which employs frustrated total internal reflection on a highly localized scale by means of a sharp dielectric tip. The coupling of the evanescent field to the sub-micrometer probe as a function of probe-sample distance, angle of incidence

  7. A three-photon microscope with adaptive optics for deep-tissue in vivo structural and functional brain imaging

    Science.gov (United States)

    Tao, Xiaodong; Lu, Ju; Lam, Tuwin; Rodriguez, Ramiro; Zuo, Yi; Kubby, Joel

    2017-02-01

    We developed a three-photon adaptive optics add-on to a commercial two-photon laser scanning microscope. We demonstrated its capability for structural and functional imaging of neurons labeled with genetically encoded red fluorescent proteins or calcium indicators deep in the living mouse brain with cellular and subcellular resolution.

  8. Adaptive Optics Technology for High-Resolution Retinal Imaging

    Science.gov (United States)

    Lombardo, Marco; Serrao, Sebastiano; Devaney, Nicholas; Parravano, Mariacristina; Lombardo, Giuseppe

    2013-01-01

    Adaptive optics (AO) is a technology used to improve the performance of optical systems by reducing the effects of optical aberrations. The direct visualization of the photoreceptor cells, capillaries and nerve fiber bundles represents the major benefit of adding AO to retinal imaging. Adaptive optics is opening a new frontier for clinical research in ophthalmology, providing new information on the early pathological changes of the retinal microstructures in various retinal diseases. We have reviewed AO technology for retinal imaging, providing information on the core components of an AO retinal camera. The most commonly used wavefront sensing and correcting elements are discussed. Furthermore, we discuss current applications of AO imaging to a population of healthy adults and to the most frequent causes of blindness, including diabetic retinopathy, age-related macular degeneration and glaucoma. We conclude our work with a discussion on future clinical prospects for AO retinal imaging. PMID:23271600

  9. Frequency based design of modal controllers for adaptive optics systems.

    Science.gov (United States)

    Agapito, Guido; Battistelli, Giorgio; Mari, Daniele; Selvi, Daniela; Tesi, Alberto; Tesi, Pietro

    2012-11-19

    This paper addresses the problem of reducing the effects of wavefront distortions in ground-based telescopes within a "Modal-Control" framework. The proposed approach allows the designer to optimize the Youla parameter of a given modal controller with respect to a relevant adaptive optics performance criterion defined on a "sampled" frequency domain. This feature makes it possible to use turbulence/vibration profiles of arbitrary complexity (even empirical power spectral densities from data), while keeping the controller order at a moderate value. Effectiveness of the proposed solution is also illustrated through an adaptive optics numerical simulator.

  10. Adaptive optics simulation performance improvements using reconfigurable logic.

    Science.gov (United States)

    Basden, Alastair

    2007-02-20

    A technique used to accelerate an adaptive optics simulation platform using reconfigurable logic is described. The performance of parts of this simulation has been improved by up to 600 times (reducing computation times by this factor) by implementing algorithms within hardware and enables adaptive optics simulations to be carried out in a reasonable time scale. This demonstrates that it is possible to use reconfigurable logic to accelerate computational codes by very large factors when compared with conventional software approaches, and this has relevance for many computationally intensive applications. The use of reconfigurable logic for high performance computing is currently in its infancy and has never before been applied to this field.

  11. Holographic fluorescence microscopy with incoherent digital holographic adaptive optics.

    Science.gov (United States)

    Jang, Changwon; Kim, Jonghyun; Clark, David C; Lee, Seungjae; Lee, Byoungho; Kim, Myung K

    2015-01-01

    Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: a wavefront sensor, wavefront corrector, and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, for example, lenslet arrays for sensing or multiactuator deformable mirrors for correcting. We have previously introduced an alternate approach based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile are possible not only with conventional coherent digital holography, but also with a new type of digital holography using incoherent light: selfinterference incoherent digital holography (SIDH). The SIDH generates a complex—i.e., amplitude plus phase—hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. Adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media.

  12. Characterization and Operation of Liquid Crystal Adaptive Optics Phoropter

    Energy Technology Data Exchange (ETDEWEB)

    Awwal, A; Bauman, B; Gavel, D; Olivier, S; Jones, S; Hardy, J L; Barnes, T; Werner, J S

    2003-02-05

    Adaptive optics (AO), a mature technology developed for astronomy to compensate for the effects of atmospheric turbulence, can also be used to correct the aberrations of the eye. The classic phoropter is used by ophthalmologists and optometrists to estimate and correct the lower-order aberrations of the eye, defocus and astigmatism, in order to derive a vision correction prescription for their patients. An adaptive optics phoropter measures and corrects the aberrations in the human eye using adaptive optics techniques, which are capable of dealing with both the standard low-order aberrations and higher-order aberrations, including coma and spherical aberration. High-order aberrations have been shown to degrade visual performance for clinical subjects in initial investigations. An adaptive optics phoropter has been designed and constructed based on a Shack-Hartmann sensor to measure the aberrations of the eye, and a liquid crystal spatial light modulator to compensate for them. This system should produce near diffraction-limited optical image quality at the retina, which will enable investigation of the psychophysical limits of human vision. This paper describes the characterization and operation of the AO phoropter with results from human subject testing.

  13. Adaptive optical microscope for brain imaging in vivo

    Science.gov (United States)

    Wang, Kai

    2017-04-01

    The optical heterogeneity of biological tissue imposes a major limitation to acquire detailed structural and functional information deep in the biological specimens using conventional microscopes. To restore optimal imaging performance, we developed an adaptive optical microscope based on direct wavefront sensing technique. This microscope can reliably measure and correct biological samples induced aberration. We demonstrated its performance and application in structural and functional brain imaging in various animal models, including fruit fly, zebrafish and mouse.

  14. Surface Plasmon Wave Adapter Designed with Transformation Optics

    DEFF Research Database (Denmark)

    Zhang, Jingjing; Xiao, Sanshui; Wubs, Martijn

    2011-01-01

    On the basis of transformation optics, we propose the design of a surface plasmon wave adapter which confines surface plasmon waves on non-uniform metal surfaces and enables adiabatic mode transformation of surface plasmon polaritons with very short tapers. This adapter can be simply achieved...... with homogeneous anisotropic naturally occurring materials or subwavelength grating-structured dielectric materials. Full wave simulations based on a finite-element method have been performed to validate our proposal....

  15. Adaptation of the lateral distal femur DXA scan technique to adults with disabilities.

    Science.gov (United States)

    Henderson, Richard C; Henderson, Brent A; Kecskemethy, Heidi H; Hidalgo, Sebastian T; Nikolova, Beth Ann; Sheridan, Kevin; Harcke, H Theodore; Thorpe, Deborah E

    2015-01-01

    The technique that best addresses the challenges of assessing bone mineral density in children with neuromuscular impairments is a dual-energy X-ray absorptiometry (DXA) scan of the lateral distal femur. The purpose of this study was to adapt this technique to adults with neuromuscular impairments and to assess the reproducibility of these measurements. Thirty-one adults with cerebral palsy had both distal femurs scanned twice, with the subject removed and then repositioned between each scan (62 distal femurs, 124 scans). Each scan was independently analyzed twice by 3 different technologists of varying experience with DXA (744 analyses). Precision of duplicate analyses of the same scan was good (range: 0.4%-2.3%) and depended on both the specific region of interest and the experience of the technologist. Precision was reduced when comparing duplicate scans, ranging from 7% in the metaphyseal (cancellous) region to 2.5% in the diaphyseal (cortical) region. The least significant change was determined as recommended by the International Society for Clinical Densitometry for each technologist and each region of interest. Obtaining reliable, reproducible, and clinically relevant assessments of bone mineral density in adults with neuromuscular impairments can be challenging. The technique of obtaining DXA scans of the lateral distal femur can be successfully applied to this population but requires a commitment to developing the necessary expertise. Copyright © 2015 The International Society for Clinical Densitometry. Published by Elsevier Inc. All rights reserved.

  16. Photonic crystal-adaptive optical devices

    DEFF Research Database (Denmark)

    Buss, Thomas

    through windows into the room. It is shown that gratings with disorder introduced to the period effectively modify the diffraction characteristics from distinct sharp and wavelength dependent orders into a broad distributions over large angular range and with sufficient mixing such that color effects...... are minimized, thus allowing a homogeneous, glare-free, white-light daylighting into the room. Even more functionality can be achieved when the optical effects are tunable or reconfigurable. This is investigated with photonic crystal dye lasers. These lasers combine a photonic crystal resonator with a dye...

  17. Solar multi-conjugate adaptive optics based on high order ground layer adaptive optics and low order high altitude correction.

    Science.gov (United States)

    Zhang, Lanqiang; Guo, Youming; Rao, Changhui

    2017-02-20

    Multi-conjugate adaptive optics (MCAO) is the most promising technique currently developed to enlarge the corrected field of view of adaptive optics for astronomy. In this paper, we propose a new configuration of solar MCAO based on high order ground layer adaptive optics and low order high altitude correction, which result in a homogeneous correction effect in the whole field of view. An individual high order multiple direction Shack-Hartmann wavefront sensor is employed in the configuration to detect the ground layer turbulence for low altitude correction. Furthermore, the other low order multiple direction Shack-Hartmann wavefront sensor supplies the wavefront information caused by high layers' turbulence through atmospheric tomography for high altitude correction. Simulation results based on the system design at the 1-meter New Vacuum Solar Telescope show that the correction uniform of the new scheme is obviously improved compared to conventional solar MCAO configuration.

  18. Three-dimensional optical transfer functions in the aberration-corrected scanning transmission electron microscope.

    Science.gov (United States)

    Jones, L; Nellist, P D

    2014-05-01

    In the scanning transmission electron microscope, hardware aberration correctors can now correct for the positive spherical aberration of round electron lenses. These correctors make use of nonround optics such as hexapoles or octupoles, leading to the limiting aberrations often being of a nonround type. Here we explore the effect of a number of potential limiting aberrations on the imaging performance of the scanning transmission electron microscope through their resulting optical transfer functions. In particular, the response of the optical transfer function to changes in defocus are examined, given that this is the final aberration to be tuned just before image acquisition. The resulting three-dimensional optical transfer functions also allow an assessment of the performance of a system for focal-series experiments or optical sectioning applications. © 2014 The Authors Journal of Microscopy © 2014 Royal Microscopical Society.

  19. Single-body lensed-fiber scanning probe actuated by magnetic force for optical imaging.

    Science.gov (United States)

    Min, Eun Jung; Na, Jihoon; Ryu, Seon Young; Lee, Byeong Ha

    2009-06-15

    We propose a fiber-based hand-held scanning probe suitable for the sample arm of an optical imaging system including optical coherence tomography. To achieve compactness, a single-body lensed-fiber and a solenoid actuator were utilized. The focusing lens of the probe was directly formed onto the distal end of a fiber, which eliminated the need for additional optical components and optical alignment. A ferromagnetic iron bead was glued onto the middle of the fiber to enable actuation by magnetic force, which allowed easy fabrication and good practicality. The fiber piece having the built-in fiber lens was forced to oscillate in its resonant frequency. With the implemented probe, optical coherence tomography images of a human fingertip and a pearl were obtained at an imaging speed of 30 frames/s over a scanning range of 4 mm.

  20. Phantom verification for a ring-scanning and prone diffuse optical imaging system

    Science.gov (United States)

    Yu, Jhao-Ming; Pan, Min-Chun; Chen, Liang-Yu; Pan, Min-Cheng; Hsu, Ya-Fen

    2017-12-01

    In this study, we proposed and implemented a ring-scanning mechanism in the prone position for application in breast tumour detection. The current scanning module using two zones with three light sources in each zone enables the acquisition of 36 source and 30 detection data (36S × 30D) items during the optical information collection phase. This study employed only three photomultiplier tubes (PMTs), instead of 30 PMTs used in a fixed model. In particular, the circular scanning of source-and-detection module actually behaves as more channels and can acquire more optoelectrical data as the scanning module operates in a fractional motion of a single channel-to-channel span. In this study, the optoelectrical measurement system was first calibrated; then, the feasibility of optical-coefficient image reconstruction was verified using several heterogeneous cylindrical phantoms. The reconstructed μa and μs ‧ images through multilayer scanning presented good outcomes, implying that the developed system is promising for 3D scanning of breasts. In a quantitative analysis, the contrast-to-noise ratios of the μa and μs ‧ images (6.00 and 4.97, respectively) for the flexible scanning scheme were superior to those derived for the fixed scheme (5.05 and 4.31, respectively). This indicates that the higher amount of detection information obtained through the proposed scanning module can enhance the spatial resolution of the reconstructed images while retaining an acceptable scanning time.

  1. The New Weather Radar for America's Space Program in Florida: A Temperature Profile Adaptive Scan Strategy

    Science.gov (United States)

    Carey, L. D.; Petersen, W. A.; Deierling, W.; Roeder, W. P.

    2009-01-01

    A new weather radar is being acquired for use in support of America s space program at Cape Canaveral Air Force Station, NASA Kennedy Space Center, and Patrick AFB on the east coast of central Florida. This new radar replaces the modified WSR-74C at Patrick AFB that has been in use since 1984. The new radar is a Radtec TDR 43-250, which has Doppler and dual polarization capability. A new fixed scan strategy was designed to best support the space program. The fixed scan strategy represents a complex compromise between many competing factors and relies on climatological heights of various temperatures that are important for improved lightning forecasting and evaluation of Lightning Launch Commit Criteria (LCC), which are the weather rules to avoid lightning strikes to in-flight rockets. The 0 C to -20 C layer is vital since most generation of electric charge occurs within it and so it is critical in evaluating Lightning LCC and in forecasting lightning. These are two of the most important duties of 45 WS. While the fixed scan strategy that covers most of the climatological variation of the 0 C to -20 C levels with high resolution ensures that these critical temperatures are well covered most of the time, it also means that on any particular day the radar is spending precious time scanning at angles covering less important heights. The goal of this project is to develop a user-friendly, Interactive Data Language (IDL) computer program that will automatically generate optimized radar scan strategies that adapt to user input of the temperature profile and other important parameters. By using only the required scan angles output by the temperature profile adaptive scan strategy program, faster update times for volume scans and/or collection of more samples per gate for better data quality is possible, while maintaining high resolution at the critical temperature levels. The temperature profile adaptive technique will also take into account earth curvature and refraction

  2. ING Workshop on Adaptive-Optics Assisted Integral Field Spectroscopy

    Science.gov (United States)

    Rutten, R. G. M.

    2005-12-01

    In May 2005 a three-day workshop took place on adaptive-optics assisted the above subject. The main reason for organising this workshop was the commissioning of the OASIS integral field spectrograph on the WHT and the latest project to augment the use of AO on the WHT with a laser beacon system.

  3. A Status Report on the Thirty Meter Telescope Adaptive Optics ...

    Indian Academy of Sciences (India)

    2016-01-27

    Jan 27, 2016 ... We provide an update on the recent development of the adaptive optics (AO) systems for the Thirty Meter Telescope (TMT) since mid-2011. The first light AO facility for TMT consists of the Narrow Field Infra-Red AO System (NFIRAOS) and the associated Laser Guide Star Facility (LGSF). This order 60 × 60 ...

  4. Laser guide stars and adaptive optics for astronomy

    Energy Technology Data Exchange (ETDEWEB)

    Max, C.E. [ed.

    1992-07-15

    Five papers are included: feasibility experiment for sodium-alyer laser guide stars at LLNL; system design for a high power sodium beacon laser; sodium guide star adaptive optics system for astronomical imaging in the visible and near-infrared; high frame-rate, large field wavefront sensor; and resolution limits for ground-based astronomical imaging. Figs, tabs, refs.

  5. Development of a backscattering type ultraviolet apertureless near-field scanning optical microscope.

    Science.gov (United States)

    Kwon, Sangjin; Jeong, Hyun; Jeong, Mun Seok; Jeong, Sungho

    2011-08-01

    A backscattering type ultraviolet apertureless near-field scanning optical microscope (ANSOM) for the correlated measurement of topographical and optical characteristics of photonic materials with high optical resolution was developed. The near-field Rayleigh scattering image of GaN covered with periodic submicron Cr dots showed that optical resolution around 40 nm was achievable. By measuring the tip scattered photoluminescence of InGaN/GaN multi quantum wells, the applicability of the developed microscope for high resolution fluorescence measurement was also demonstrated.

  6. Optimization of an Adaptive SPECT System with the Scanning Linear Estimator.

    Science.gov (United States)

    Ghanbari, Nasrin; Clarkson, Eric; Kupinski, Matthew; Li, Xin

    2017-09-01

    A method for optimization of an adaptive Single Photon Emission Computed Tomography (SPECT) system is presented. Adaptive imaging systems can quickly change their hardware configuration in response to data being generated in order to improve image quality for a specific task. In this work we simulate an adaptive SPECT system and propose a method for finding the adaptation that maximizes the performance on a signal estimation task. To start with, a simulated object model containing a spherical signal is imaged with a scout configuration. A Markov-Chain Monte Carlo (MCMC) technique utilizes the scout data to generate an ensemble of possible objects consistent with the scout data. This object ensemble is imaged by numerous simulated hardware configurations and for each system estimates of signal activity, size and location are calculated via the Scanning Linear Estimator (SLE). A figure of merit, based on a Modified Dice Index (MDI), quantifies the performance of each imaging configuration and it allows for optimization of the adaptive SPECT. This figure of merit is calculated by multiplying two terms: the first term uses the definition of the Dice similarity index to determine the percent of overlap between the actual and the estimated spherical signal, the second term utilizes an exponential function that measures the squared error for the activity estimate. The MDI combines the error in estimates of activity, size, and location, in one convenient metric and it allows for simultaneous optimization of the SPECT system with respect to all the estimated signal parameters. The results of our optimizations indicate that the adaptive system performs better than a non-adaptive one in conditions where the diagnostic scan has a low photon count - on the order of thousand photons per projection. In a statistical study, we optimized the SPECT system for one hundred unique objects and demonstrated that the average MDI on an estimation task is 0.84 for the adaptive system and 0

  7. Sapc - Application for Adapting Scanned Analogue Photographs to Use Them in Structure from Motion Technology

    Science.gov (United States)

    Salach, A.

    2017-05-01

    The documentary value of analogue scanned photographs is invaluable. A large and rich collection of archival photographs is often the only source of information about past of the selected area. This paper presents a method of adaptation of scanned, analogue photographs to suitable form allowing to use them in Structure from Motion technology. For this purpose, an automatic algorithm, implemented in the application called SAPC (Scanned Aerial Photographs Correction), which transforms scans to a form, which characteristic similar to the images captured by a digital camera, was invented. Images, which are created in the applied program as output data, are characterized by the same principal point position in each photo and the same resolution through cutting out the black photo frame. Additionally, SAPC generates a binary image file, which can mask areas of fiducial marks. In the experimental section, scanned, analogue photographs of Warsaw, which had been captured in 1986, were used in two variants: unprocessed and processed in SAPC application. An insightful analysis was conducted on the influence of transformation in SAPC on quality of spatial orientation of photographs. Block adjustment through aerial triangulation was calculated using two SfM software products: Agisoft PhotoScan and Pix4d and their results were compared with results obtained from professional photogrammetric software - Trimble Inpho. The author concluded that pre-processing in SAPC application had a positive impact on a quality of block orientation of scanned, analogue photographs, using SfM technology.

  8. Selective Plane Illumination Differential Dynamic Microscopy with Adaptive Optics

    Science.gov (United States)

    Wulstein, Devynn; McGorty, Ryan

    We measure the dynamics of colloidal particles and DNA molecules using differential dynamic microscopy (DDM) on images captured through selective-plane illumination microscopy (SPIM). Combining DDM, a digital Fourier microscopy method, and SPIM, an optical sectioning microscopy technique, we can analyze the dynamics of concentrated suspensions of colloids and biopolymers. Further, selective-plane illumination differential dynamic microscopy (SPIDDM) exploits the spatial variations of the Gaussian light-sheet to obtain diffusion data over a wide range of spatial frequencies. Presented work focuses on in vitro measurements of colloids, DNA molecules and cytoskeleton networks. We have measured the collective dynamics of DNA in actin and microtubule networks spanning an order of magnitude in spatial frequencies. This work could easily extend to living samples given SPIDDM's sparing use of excitation light. We are currently adding adaptive optics into our light-sheet microscope with a deformable mirror. We discuss using adaptive optics for multiple purposes. The mirror corrects optical aberrations due to the sample holder and the sample. We are also using adaptive optics to optimize the three-dimensional point spread function for DDM measurements. Using the deformable mirror to purposefully introduce known aberrations could allow for a more precise measurement of colloidal or molecular dynamics in three-dimensions.

  9. Scanning laser topography and scanning laser polarimetry: comparing both imaging methods at same distances from the optic nerve head.

    Science.gov (United States)

    Kremmer, Stephan; Keienburg, Marcus; Anastassiou, Gerasimos; Schallenberg, Maurice; Steuhl, Klaus-Peter; Selbach, J Michael

    2012-01-01

    To compare the performance of scanning laser topography (SLT) and scanning laser polarimetry (SLP) on the rim of the optic nerve head and its surrounding area and thereby to evaluate whether these imaging technologies are influenced by other factors beyond the thickness of the retinal nerve fiber layer (RNFL). A total of 154 eyes from 5 different groups were examined: young healthy subjects (YNorm), old healthy subjects (ONorm), patients with normal tension glaucoma (NTG), patients with open-angle glaucoma and early glaucomatous damage (OAGE) and patients with open-angle glaucoma and advanced glaucomatous damage (OAGA). SLT and SLP measurements were taken. Four concentric circles were superimposed on each of the images: the first one measuring at the rim of the optic nerve head (1.0 ONHD), the next measuring at 1.25 optic nerve head diameters (ONHD), at 1.5 ONHD and at 1.75 ONHD. The aligned images were analyzed using GDx/NFA software. Both methods showed peaks of RNFL thickness in the superior and inferior segments of the ONH. The maximum thickness, registered by the SLT device was at the ONH rim where the SLP device tended to measure the lowest values. SLT measurements at the ONH were influenced by other tissues besides the RNFL like blood vessels and glial tissues. SLT and SLP were most strongly correlated at distances of 1.25 and 1.5 ONHD. While both imaging technologies are valuable tools in detecting glaucoma, measurements at the ONH rim should be interpreted critically since both methods might provide misleading results. For the assessment of the retinal nerve fiber layer we would like to recommend for both imaging technologies, SLT and SLP, measurements in 1.25 and 1.5 ONHD distance of the rim of the optic nerve head.

  10. Wavefront sensorless adaptive optics versus sensor-based adaptive optics for in vivo fluorescence retinal imaging (Conference Presentation)

    Science.gov (United States)

    Wahl, Daniel J.; Zhang, Pengfei; Jian, Yifan; Bonora, Stefano; Sarunic, Marinko V.; Zawadzki, Robert J.

    2017-02-01

    Adaptive optics (AO) is essential for achieving diffraction limited resolution in large numerical aperture (NA) in-vivo retinal imaging in small animals. Cellular-resolution in-vivo imaging of fluorescently labeled cells is highly desirable for studying pathophysiology in animal models of retina diseases in pre-clinical vision research. Currently, wavefront sensor-based (WFS-based) AO is widely used for retinal imaging and has demonstrated great success. However, the performance can be limited by several factors including common path errors, wavefront reconstruction errors and an ill-defined reference plane on the retina. Wavefront sensorless (WFS-less) AO has the advantage of avoiding these issues at the cost of algorithmic execution time. We have investigated WFS-less AO on a fluorescence scanning laser ophthalmoscopy (fSLO) system that was originally designed for WFS-based AO. The WFS-based AO uses a Shack-Hartmann WFS and a continuous surface deformable mirror in a closed-loop control system to measure and correct for aberrations induced by the mouse eye. The WFS-less AO performs an open-loop modal optimization with an image quality metric. After WFS-less AO aberration correction, the WFS was used as a control of the closed-loop WFS-less AO operation. We can easily switch between WFS-based and WFS-less control of the deformable mirror multiple times within an imaging session for the same mouse. This allows for a direct comparison between these two types of AO correction for fSLO. Our results demonstrate volumetric AO-fSLO imaging of mouse retinal cells labeled with GFP. Most significantly, we have analyzed and compared the aberration correction results for WFS-based and WFS-less AO imaging.

  11. The AVES adaptive optics spectrograph for the VLT: status report

    Science.gov (United States)

    Pallavicini, Roberto; Delabre, Bernard; Pasquini, Luca; Zerbi, Filippo M.; Bonanno, Giovanni; Comari, Maurizio; Conconi, Paolo; Mazzoleni, Ruben; Santin, Paolo; Damiani, Francesco; Di Marcantonio, Paolo; Franchini, Mariagrazia; Spano, Paolo; Bonifacio, P.; Catalano, Santo; Molaro, Paolo P.; Randich, S.; Rodono, Marcello

    2003-03-01

    We report on the status of AVES, the Adaptive-optics Visual Echelle Spectrograph proposed for the secondary port of the Nasmyth Adaptive Optics System (NAOS) recently installed at the VLT. AVES is an intermediate resolution (R ≍ 16,000) high-efficiency fixed- format echelle spectrograph which operates in the spectral band 500 - 1,000 nm. In addition to a high intrinsic efficiency, comparable to that of ESI at Keck II, it takes advantage of the adaptive optics correction provided by NAOS to reduce the sky and detector contribution in background-limited observations of weak sources, thus allowing a further magnitude gain with respect to comparable non-adaptive optics spectrographs. Simulations show that the instrument will be capable of reaching a magnitude V = 22.5 at S/N > 10 in two hours, two magnitudes weaker than GIRAFFE at the same resolution and 3 magnitudes weaker than the higher resolution UVES spectrograph. Imaging and coronographic functions have also been implemented in the design. We present the results of the final design study and we dicuss the technical and operational issues related to its implementation at the VLT as a visitor instrument. We also discuss the possibility of using a scaled-up non-adaptive optics version of the same design as an element of a double- or triple-arm intermediate-resolution spectrograph for the VLT. Such an option looks attractive in the context of a high-efficiency large-bandwidth (320 - 1,500 nm) spectrograph ("fast-shooter") being considered by ESO as a 2nd-generation VLT instrument.

  12. Adaptive optics high resolution spectroscopy: present status and future direction

    Energy Technology Data Exchange (ETDEWEB)

    Alcock, C; Angel, R; Ciarlo, D; Fugate, R O; Ge, J; Kuzmenko, P; Lloyd-Hart, M; Macintosh, B; Najita, J; Woolf, N

    1999-07-27

    High resolution spectroscopy experiments with visible adaptive optics (AO) telescopes at Starfire Optical Range and Mt. Wilson have demonstrated that spectral resolution can be routinely improved by a factor of - 10 over the seeing-limited case with no extra light losses at visible wavelengths. With large CCDs now available, a very wide wavelength range can be covered in a single exposure. In the near future, most large ground-based telescopes will be equipped with powerful A0 systems. Most of these systems are aimed primarily at diffraction-limited operation in the near IR. An exciting new opportunity will thus open up for high resolution IR spectroscopy. Immersion echelle gratings with much coarser grooves being developed by us at LLNL will play a critical role in achieving high spectral resolution with a compact and low cost IR cryogenically cooled spectrograph and simultaneous large wavelength coverage on relatively small IR detectors. We have constructed a new A0 optimized spectrograph at Steward Observatory to provide R = 200,000 in the optical, which is being commissioned at the Starfire Optical Range 3.5m telescope. We have completed the optical design of the LLNL IR Immersion Spectrograph (LISPEC) to take advantage of improved silicon etching technology. Key words: adaptive optics, spectroscopy, high resolution, immersion gratings

  13. MICADO: the E-ELT adaptive optics imaging camera : The E-ELT adaptive optics imaging camera

    NARCIS (Netherlands)

    Davies, Richard; Ageorges, N.; Barl, L.; Bedin, L. R.; Bender, R.; Bernardi, P.; Chapron, F.; Clenet, Y.; Deep, A.; Deul, E.; Drost, M.; Eisenhauer, F.; Falomo, R.; Fiorentino, G.; Förster Schreiber, N. M.; Gendron, E.; Genzel, R.; Gratadour, D.; Greggio, L.; Grupp, F.; Held, E.; Herbst, T.; Hess, H.-J.; Hubert, Z.; Jahnke, K.; Kuijken, K.; Lutz, D.; Magrin, D.; Muschielok, B.; Navarro, R.; Noyola, E.; Paumard, T.; Piotto, G.; Ragazzoni, R.; Renzini, A.; Rousset, G.; Rix, H.-W.; Saglia, R.; Tacconi, L.; Thiel, M.; Tolstoy, E.; Trippe, S.; Tromp, N.; Valentijn, E. A.; Verdoes Kleijn, G.; Wegner, M.; McLean, I.S.; Ramsay, S.K.; Takami, H.

    MICADO is the adaptive optics imaging camera for the E-ELT. It has been designed and optimised to be mounted to the LGS-MCAO system MAORY, and will provide diffraction limited imaging over a wide (~1 arcmin) field of view. For initial operations, it can also be used with its own simpler AO module

  14. Digital and optical systolic architectures for airborne adaptive radars

    Science.gov (United States)

    Lis, Stanley; Vannicola, Vincent C.; Graniero, John A.; Medoff, Barry P.; Penn, William A.

    1986-07-01

    Systolic architectures for digital and analog, electronic and optical signal processing are presented along with specific applications to adaptive nulling. It is shown how the various architectures provide for the implementation of adaptive algorithms and how technologies affect performance. Their effects on adaptive degrees of freedom, convergence time, null depth, signal to noise ratio are presented along with size, weight, and required power. Adaptive algorithms covered are of two basic types: feedback/iterative and direct methods. Examples of each include the least mean square (LMS) for the iterative type and the QU factorization based on the Givens method for the direct method. Simulation results have verified the performance of the least squares and the systolic array for QU factorization by Givens method. Improved performance was obtained using the modified minimum variance distortionless response algorithm based on the maximum likelihood criteria. An optical implementation of the least squares algorithm over a continuously adaptive multi-path was experimentally evaluated. Thus far, 24 dB of cancellation was achieved over a 7 micro-second multi-path window for 10 mega-Hertz instantaneous bandwidth. Adaptivity in the spatial, temporal and Doppler domains are illustrated and their embodiment into the various architectures are presented. For example, an analog optical processor which generates weights in the spatial and temporal (multi-path) domains for broadband systems is shown. Also shown is a digital systolic architecture which is applied to a direct decomposition method for generation of adaptive weights in the spatial and Doppler domains. A description of brassboard models representing both architectures is included.

  15. Algorithm for localized adaptive diffuse optical tomography and its application in bioluminescence tomography

    Science.gov (United States)

    Naser, Mohamed A.; Patterson, Michael S.; Wong, John W.

    2014-04-01

    A reconstruction algorithm for diffuse optical tomography based on diffusion theory and finite element method is described. The algorithm reconstructs the optical properties in a permissible domain or region-of-interest to reduce the number of unknowns. The algorithm can be used to reconstruct optical properties for a segmented object (where a CT-scan or MRI is available) or a non-segmented object. For the latter, an adaptive segmentation algorithm merges contiguous regions with similar optical properties thereby reducing the number of unknowns. In calculating the Jacobian matrix the algorithm uses an efficient direct method so the required time is comparable to that needed for a single forward calculation. The reconstructed optical properties using segmented, non-segmented, and adaptively segmented 3D mouse anatomy (MOBY) are used to perform bioluminescence tomography (BLT) for two simulated internal sources. The BLT results suggest that the accuracy of reconstruction of total source power obtained without the segmentation provided by an auxiliary imaging method such as x-ray CT is comparable to that obtained when using perfect segmentation.

  16. Imaging retinal nerve fiber bundles using optical coherence tomography with adaptive optics.

    Science.gov (United States)

    Kocaoglu, Omer P; Cense, Barry; Jonnal, Ravi S; Wang, Qiang; Lee, Sangyeol; Gao, Weihua; Miller, Donald T

    2011-08-15

    Early detection of axonal tissue loss in retinal nerve fiber layer (RNFL) is critical for effective treatment and management of diseases such as glaucoma. This study aims to evaluate the capability of ultrahigh-resolution optical coherence tomography with adaptive optics (UHR-AO-OCT) for imaging the RNFL axonal bundles (RNFBs) with 3×3×3μm(3) resolution in the eye. We used a research-grade UHR-AO-OCT system to acquire 3°×3° volumes in four normal subjects and one subject with an arcuate retinal nerve fiber layer defect (n=5; 29-62years). Cross section (B-scans) and en face (C-scan) slices extracted from the volumes were used to assess visibility and size distribution of individual RNFBs. In one subject, we reimaged the same RNFBs twice over a 7month interval and compared bundle width and thickness between the two imaging sessions. Lastly we compared images of an arcuate RNFL defect acquired with UHR-AO-OCT and commercial OCT (Heidelberg Spectralis). Individual RNFBs were distinguishable in all subjects at 3° retinal eccentricity in both cross-sectional and en face views (width: 30-50μm, thickness: 10-15μm). At 6° retinal eccentricity, RNFBs were distinguishable in three of the five subjects in both views (width: 30-45μm, thickness: 20-40μm). Width and thickness RNFB measurements taken 7months apart were strongly correlated (p<0.0005). Mean difference and standard deviation of the differences between the two measurement sessions were -0.1±4.0μm (width) and 0.3±1.5μm (thickness). UHR-AO-OCT outperformed commercial OCT in terms of clarity of the microscopic retina. To our knowledge, these are the first measurements of RNFB cross section reported in the living human eye. Copyright © 2011 Elsevier Ltd. All rights reserved.

  17. Proton irradiation of liquid crystal based adaptive optical devices

    Energy Technology Data Exchange (ETDEWEB)

    Buis, E.J., E-mail: ernst-jan.buis@tno.nl [cosine Science and Computing BV, Niels Bohrweg 11, 2333 CA Leiden (Netherlands); Berkhout, G.C.G. [cosine Science and Computing BV, Niels Bohrweg 11, 2333 CA Leiden (Netherlands); Huygens Laboratory, Leiden University, P.O. Box 9504, 2300 RA Leiden (Netherlands); Love, G.D.; Kirby, A.K.; Taylor, J.M. [Department of Physics, Durham University, South Road, Durham DH1 3LE (United Kingdom); Hannemann, S.; Collon, M.J. [cosine Research BV, Niels Bohrweg 11, 2333 CA Leiden (Netherlands)

    2012-01-01

    To assess its radiation hardness, a liquid crystal based adaptive optical element has been irradiated using a 60 MeV proton beam. The device with the functionality of an optical beam steerer was characterised before, during and after the irradiation. A systematic set of measurements on the transmission and beam deflection angles was carried out. The measurements showed that the transmission decreased only marginally and that its optical performance degraded only after a very high proton fluence (10{sup 10}p/cm{sup 2}). The device showed complete annealing in the functionality as a beam steerer, which leads to the conclusion that the liquid crystal technology for optical devices is not vulnerable to proton irradiation as expected in space.

  18. Optic flow improves adaptability of spatiotemporal characteristics during split-belt locomotor adaptation with tactile stimulation.

    Science.gov (United States)

    Eikema, Diderik Jan A; Chien, Jung Hung; Stergiou, Nicholas; Myers, Sara A; Scott-Pandorf, Melissa M; Bloomberg, Jacob J; Mukherjee, Mukul

    2016-02-01

    Human locomotor adaptation requires feedback and feed-forward control processes to maintain an appropriate walking pattern. Adaptation may require the use of visual and proprioceptive input to decode altered movement dynamics and generate an appropriate response. After a person transfers from an extreme sensory environment and back, as astronauts do when they return from spaceflight, the prolonged period required for re-adaptation can pose a significant burden. In our previous paper, we showed that plantar tactile vibration during a split-belt adaptation task did not interfere with the treadmill adaptation however, larger overground transfer effects with a slower decay resulted. Such effects, in the absence of visual feedback (of motion) and perturbation of tactile feedback, are believed to be due to a higher proprioceptive gain because, in the absence of relevant external dynamic cues such as optic flow, reliance on body-based cues is enhanced during gait tasks through multisensory integration. In this study, we therefore investigated the effect of optic flow on tactile-stimulated split-belt adaptation as a paradigm to facilitate the sensorimotor adaptation process. Twenty healthy young adults, separated into two matched groups, participated in the study. All participants performed an overground walking trial followed by a split-belt treadmill adaptation protocol. The tactile group (TC) received vibratory plantar tactile stimulation only, whereas the virtual reality and tactile group (VRT) received an additional concurrent visual stimulation: a moving virtual corridor, inducing perceived self-motion. A post-treadmill overground trial was performed to determine adaptation transfer. Interlimb coordination of spatiotemporal and kinetic variables was quantified using symmetry indices and analyzed using repeated-measures ANOVA. Marked changes of step length characteristics were observed in both groups during split-belt adaptation. Stance and swing time symmetries were

  19. Electrically controllable ionic polymeric gels as adaptive optical lenses

    Science.gov (United States)

    Salehpoor, Karim; Shahinpoor, Mohsen; Mojarrad, Mehran

    1996-02-01

    Reversible change in optical properties of ionic polymeric gels, 2-acrylamido-2-methylpropane sulfonic acid (PAMPS) and polyacrylic acid plus sodium acrylate cross-linked with bisacrylamide (PAAM), under the effect of an electric field is reported. The shape of a cylindrical piece of the gel, with flat top and bottom surfaces, changed when affected by an electric field. The top surface became curved and the sense of the curvature (whether concave or convex) depended on the polarity of the applied electric field. The curvature of the surface changed from concave to convex and vice versa by changing the polarity of the electric field. By the use of an optical apparatus, focusing capability of the curved surface was verified and the focal length of the deformed gel was measured. The effect of the intensity of the applied electric field on the surface curvature and thus, on the focal length of the gel are tested. Different mechanisms are discussed; either of them or their combination may explain the surface deformation and curvature. Practical difficulties in the test procedure and the future potential of the electrically adaptive and active optical lenses are also discussed. These adaptive lenses may be considered as smart adaptive lenses for contact lens or other optical applications requiring focal point undulation.

  20. Scanning near-field optical microscopy on rough surfaces: Applications in chemistry, biology, and medicine

    OpenAIRE

    Kaupp, Gerd

    2006-01-01

    Shear-force apertureless scanning near-field optical microscopy (SNOM) with very sharp uncoated tapered waveguides relies on the unexpected enhancement of reflection in the shear-force gap. It is the technique for obtaining chemical (materials) contrast in the optical image of “real world” surfaces that are rough and very rough without topographical artifacts, and it is by far less complicated than other SNOM techniques that can only be used for very flat surfaces. The ex...

  1. Three-dimensional imaging of micro-specimen by optical scanning holography

    Science.gov (United States)

    Liu, Jung-Ping; Tsou, Cheng-Hao

    2017-04-01

    Optical scanning holography (OSH) is a scanning-type digital holographic technique. In OSH, a heterodyne interference pattern is generated to raster scan the object. OSH can be operated in the incoherent mode and thus is able to record a fluorescence hologram. In addition, resolution of the OSH is proportional to the density of the interference pattern. Here we use a high-NA microscope objective to generate a dynamic Fresnel zone plate to record a hologram of micro-specimen. The achieved transverse resolution and longitudinal resolution are 0.78μm and 3.1μm, respectively.

  2. Adaptive optics for improved retinal surgery and diagnostics

    Energy Technology Data Exchange (ETDEWEB)

    Humayun, M S; Sadda, S R; Thompson, C A; Olivier, S S; Kartz, M W

    2000-08-21

    It is now possible to field a compact adaptive optics (AO) system on a surgical microscope for use in retinal diagnostics and surgery. Recent developments in integrated circuit technology and optical photonics have led to the capability of building an AO system that is compact and significantly less expensive than traditional AO systems. It is foreseen that such an AO system can be integrated into a surgical microscope while maintaining a package size of a lunchbox. A prototype device can be developed in a manner that lends itself well to large-scale manufacturing.

  3. Design and verification of the miniature optical system for small object surface profile fast scanning

    Science.gov (United States)

    Chi, Sheng; Lee, Shu-Sheng; Huang, Jen, Jen-Yu; Lai, Ti-Yu; Jan, Chia-Ming; Hu, Po-Chi

    2016-04-01

    As the progress of optical technologies, different commercial 3D surface contour scanners are on the market nowadays. Most of them are used for reconstructing the surface profile of mold or mechanical objects which are larger than 50 mm×50 mm× 50 mm, and the scanning system size is about 300 mm×300 mm×100 mm. There are seldom optical systems commercialized for surface profile fast scanning for small object size less than 10 mm×10 mm×10 mm. Therefore, a miniature optical system has been designed and developed in this research work for this purpose. Since the most used scanning method of such system is line scan technology, we have developed pseudo-phase shifting digital projection technology by adopting projecting fringes and phase reconstruction method. A projector was used to project a digital fringe patterns on the object, and the fringes intensity images of the reference plane and of the sample object were recorded by a CMOS camera. The phase difference between the plane and object can be calculated from the fringes images, and the surface profile of the object was reconstructed by using the phase differences. The traditional phase shifting method was accomplished by using PZT actuator or precisely controlled motor to adjust the light source or grating and this is one of the limitations for high speed scanning. Compared with the traditional optical setup, we utilized a micro projector to project the digital fringe patterns on the sample. This diminished the phase shifting processing time and the controlled phase differences between the shifted phases become more precise. Besides, the optical path design based on a portable device scanning system was used to minimize the size and reduce the number of the system components. A screwdriver section about 7mm×5mm×5mm has been scanned and its surface profile was successfully restored. The experimental results showed that the measurement area of our system can be smaller than 10mm×10mm, the precision reached to

  4. Novel modes and adaptive block scanning order for intra prediction in AV1

    Science.gov (United States)

    Hadar, Ofer; Shleifer, Ariel; Mukherjee, Debargha; Joshi, Urvang; Mazar, Itai; Yuzvinsky, Michael; Tavor, Nitzan; Itzhak, Nati; Birman, Raz

    2017-09-01

    The demand for streaming video content is on the rise and growing exponentially. Networks bandwidth is very costly and therefore there is a constant effort to improve video compression rates and enable the sending of reduced data volumes while retaining quality of experience (QoE). One basic feature that utilizes the spatial correlation of pixels for video compression is Intra-Prediction, which determines the codec's compression efficiency. Intra prediction enables significant reduction of the Intra-Frame (I frame) size and, therefore, contributes to efficient exploitation of bandwidth. In this presentation, we propose new Intra-Prediction algorithms that improve the AV1 prediction model and provide better compression ratios. Two (2) types of methods are considered: )1( New scanning order method that maximizes spatial correlation in order to reduce prediction error; and )2( New Intra-Prediction modes implementation in AVI. Modern video coding standards, including AVI codec, utilize fixed scan orders in processing blocks during intra coding. The fixed scan orders typically result in residual blocks with high prediction error mainly in blocks with edges. This means that the fixed scan orders cannot fully exploit the content-adaptive spatial correlations between adjacent blocks, thus the bitrate after compression tends to be large. To reduce the bitrate induced by inaccurate intra prediction, the proposed approach adaptively chooses the scanning order of blocks according to criteria of firstly predicting blocks with maximum number of surrounding, already Inter-Predicted blocks. Using the modified scanning order method and the new modes has reduced the MSE by up to five (5) times when compared to conventional TM mode / Raster scan and up to two (2) times when compared to conventional CALIC mode / Raster scan, depending on the image characteristics (which determines the percentage of blocks predicted with Inter-Prediction, which in turn impacts the efficiency of the new

  5. Adaptive and real-time optimal control for adaptive optics systems

    NARCIS (Netherlands)

    Doelman, N.J.; Fraanje, P.R.; Houtzager, I.; Verhaegen, M.

    2009-01-01

    An optimal control method to reject turbulence-induced wavefront distortions in an Adaptive Optics system is discussed. Details of a data-driven control approach are presented where the emphasis is put on the estimation of the optimal predictor of the wavefront disturbance. Several algorithms

  6. Genomic scan reveals loci under altitude adaptation in Tibetan and Dahe pigs.

    Directory of Open Access Journals (Sweden)

    Kunzhe Dong

    Full Text Available High altitude environments are of particular interest in the studies of local adaptation as well as their implications in physiology and clinical medicine in human. Some Chinese pig breeds, such as Tibetan pig (TBP that is well adapted to the high altitude and Dahe pig (DHP that dwells at the moderate altitude, provide ideal materials to study local adaptation to altitudes. Yet, it is still short of in-depth analysis and understanding of the genetic adaptation to high altitude in the two pig populations. In this study we conducted a genomic scan for selective sweeps using FST to identify genes showing evidence of local adaptations in TBP and DHP, with Wuzhishan pig (WZSP as the low-altitude reference. Totally, we identified 12 specific selective genes (CCBE1, F2RL1, AGGF1, ZFPM2, IL2, FGF5, PLA2G4A, ADAMTS9, NRBF2, JMJD1C, VEGFC and ADAM19 for TBP and six (OGG1, FOXM, FLT3, RTEL1, CRELD1 and RHOG for DHP. In addition, six selective genes (VPS13A, GNA14, GDAP1, PARP8, FGF10 and ADAMTS16 were shared by the two pig breeds. Among these selective genes, three (VEGFC, FGF10 and ADAMTS9 were previously reported to be linked to the local adaptation to high altitudes in pigs, while many others were newly identified by this study. Further bioinformatics analysis demonstrated that majority of these selective signatures have some biological functions relevant to the altitude adaptation, for examples, response to hypoxia, development of blood vessels, DNA repair and several hematological involvements. These results suggest that the local adaptation to high altitude environments is sophisticated, involving numerous genes and multiple biological processes, and the shared selective signatures by the two pig breeds may provide an effective avenue to identify the common adaptive mechanisms to different altitudes.

  7. Optical properties of photoreceptor and retinal pigment epithelium cells investigated with adaptive optics optical coherence tomography

    Science.gov (United States)

    Liu, Zhuolin

    Human vision starts when photoreceptors collect and respond to light. Photoreceptors do not function in isolation though, but share close interdependence with neighboring photoreceptors and underlying retinal pigment epithelium (RPE) cells. These cellular interactions are essential for normal function of the photoreceptor-RPE complex, but methods to assess these in the living human eye are limited. One approach that has gained increased promise is high-resolution retinal imaging that has undergone tremendous technological advances over the last two decades to probe the living retina at the cellular level. Pivotal in these advances has been adaptive optics (AO) and optical coherence tomography (OCT) that together allow unprecedented spatial resolution of retinal structures in all three dimensions. Using these high-resolution systems, cone photoreceptor are now routinely imaged in healthy and diseased retina enabling fundamental structural properties of cones to be studied such as cell spacing, packing arrangement, and alignment. Other important cell properties, however, have remained elusive to investigation as even better imaging performance is required and thus has resulted in an incomplete understanding of how cells in the photoreceptor-RPE complex interact with light. To address this technical bottleneck, we expanded the imaging capability of AO-OCT to detect and quantify more accurately and completely the optical properties of cone photoreceptor and RPE cells at the cellular level in the living human retina. The first objective of this thesis was development of a new AO-OCT method that is more precise and sensitive, thus enabling a more detailed view of the 3D optical signature of the photoreceptor-RPE complex than was previously possible (Chapter 2). Using this new system, the second objective was quantifying the waveguide properties of individual cone photoreceptor inner and outer segments across the macula (Chapter 3). The third objective extended the AO

  8. Optical power allocation for adaptive transmissions in wavelength-division multiplexing free space optical networks

    Directory of Open Access Journals (Sweden)

    Hui Zhou

    2015-08-01

    Full Text Available Attracting increasing attention in recent years, the Free Space Optics (FSO technology has been recognized as a cost-effective wireless access technology for multi-Gigabit rate wireless networks. Radio on Free Space Optics (RoFSO provides a new approach to support various bandwidth-intensive wireless services in an optical wireless link. In an RoFSO system using wavelength-division multiplexing (WDM, it is possible to concurrently transmit multiple data streams consisting of various wireless services at very high rate. In this paper, we investigate the problem of optical power allocation under power budget and eye safety constraints for adaptive WDM transmission in RoFSO networks. We develop power allocation schemes for adaptive WDM transmissions to combat the effect of weather turbulence on RoFSO links. Simulation results show that WDM RoFSO can support high data rates even over long distance or under bad weather conditions with an adequate system design.

  9. Modified Acousto-Optic Adaptive Processor (Mod-AOAP)

    Science.gov (United States)

    Keefer, Christopher W.; Malowicki, John E.; Payson, Paul M.

    1992-12-01

    Multipath jamming noise introduced into a radar antenna's sidelobes effectively reduces the radar's target detection capability. The acousto-optic adaptive processor (AOAP) was developed to demonstrate an optical implementation of the least mean square (LMS) algorithm for adaptively cancelling noise jamming. The AOAP system demonstrated 30 dB cancellation of monotone CW signals, with degraded performance as the bandwidth increased. The liquid crystal light valve (LCLV), used as the integrator/ spatial light modulator in the AOAP, has been identified as limiting system performance. In this report we report on modifications to the AOAP. Specifically, the LCLV has been replaced with a photorefractive crystal. Cancellation performance and cancellation speed of the mod-AOAP are presented and compared to the original results.

  10. Investigation of whispering gallery modes in microlasers by scanning near-field optical microscopy

    Science.gov (United States)

    Polubavkina, Yu S.; Kryzhanovskaya, N. V.; Nadtochiy, A. M.; Mintairov, A. M.; Lipovsky, A. A.; Scherbak, S. A.; Kulagina, M. M.; Maximov, M. V.; Zhukov, A. E.

    2017-11-01

    Near-field scanning optical microscopy (NSOM) with a spatial resolution below the light diffraction limit was used to study intensity distributions of the whispering gallery modes (WGMs) in quantum dot-based microdisk and microring lasers on GaAs with different outer diameters. Room temperature microphotoluminescence study (μPL) reveal lasing in microlasers of both geometries.

  11. Implant planning and placement using optical scanning and cone beam CT technology

    NARCIS (Netherlands)

    van der Zel, J.M.

    2008-01-01

    There is a growing interest in minimally invasive implant therapy as a standard prosthodontic treatment, providing complete restoration of occlusal function. A new treatment method (CADDIMA), which combines both computerized tomographic (CT) and optical laser-scan data for planning and design of

  12. Observation of magnetic domains using a reflection mode scanning near-field optical microscope

    NARCIS (Netherlands)

    Durkam, C.; Shvets, I.V.; Lodder, J.C.

    1997-01-01

    It is demonstrated that it is possible to image magnetic domains with a resolution of better than 60 nm with the Kerr effect in a reflection-mode scanning near-field optical microscope. Images taken of tracks of thermomagnetically prewritten bits in a Co/Pt multilayer structure magnetized out-of

  13. Real-time non-linear image processing using an active optical scanning technique

    Science.gov (United States)

    Duncan, B. D.; Poon, T.-C.; Pieper, R. J.

    1991-02-01

    Real-time non-linear image processing has been achieved using an active optical scanning technique. This paper reports experimental results in edge extraction for both binary and grey-scale transmissive objects. Binary edge extractionis achieved using morphological transformations, while grey-scale edge extraction is achieved using a threshold decomposition technique. Advantages and limitation of both techniques are identified.

  14. Combining optical tweezers and scanning probe microscopy to study DNA-protein interactions

    NARCIS (Netherlands)

    Huisstede, Jurgen H G; Subramaniam, Vinod; Bennink, Martin L

    We present the first results obtained with a new instrument designed and built to study DNA-protein interactions at the single molecule level. This microscope combines optical tweezers with scanning probe microscopy and allows us to locate DNA-binding proteins on a single suspended DNA molecule. A

  15. Near-Field Scanning Optical Microscopy of Single Fluorescent Dendritic Molecules

    NARCIS (Netherlands)

    Veerman, J.A.; Levi, S.; van Veggel, F.C.J.M.; Reinhoudt, David; van Hulst, N.F.

    1999-01-01

    Individual dendritic molecules adsorbed o­n glass containing a single fluorescent rhodamine B core have been observed with near-field scanning optical microscopy (NSOM); height and fluorescence images were obtained simultaneously. The dendritic assemblies can be discriminated from free fluorescent

  16. Protecting the entanglement of twisted photons by adaptive optics

    Science.gov (United States)

    Leonhard, Nina; Sorelli, Giacomo; Shatokhin, Vyacheslav N.; Reinlein, Claudia; Buchleitner, Andreas

    2018-01-01

    We study the efficiency of adaptive optics (AO) correction for the free-space propagation of entangled photonic orbital-angular-momentum (OAM) qubit states to reverse moderate atmospheric turbulence distortions. We show that AO can significantly reduce crosstalk to modes within and outside the encoding subspace and thereby stabilize entanglement against turbulence. This method establishes a reliable quantum channel for OAM photons in turbulence, and it enhances the threshold turbulence strength for secure quantum communication by at least a factor 2.

  17. Adaptive-optics Optical Coherence Tomography Processing Using a Graphics Processing Unit*

    OpenAIRE

    Shafer, Brandon A.; Kriske, Jeffery E.; Kocaoglu, Omer P.; Turner, Timothy L.; Liu, Zhuolin; Lee, John Jaehwan; Miller, Donald T.

    2014-01-01

    Graphics processing units are increasingly being used for scientific computing for their powerful parallel processing abilities, and moderate price compared to super computers and computing grids. In this paper we have used a general purpose graphics processing unit to process adaptive-optics optical coherence tomography (AOOCT) images in real time. Increasing the processing speed of AOOCT is an essential step in moving the super high resolution technology closer to clinical viability.

  18. Adaptive-optics optical coherence tomography processing using a graphics processing unit.

    Science.gov (United States)

    Shafer, Brandon A; Kriske, Jeffery E; Kocaoglu, Omer P; Turner, Timothy L; Liu, Zhuolin; Lee, John Jaehwan; Miller, Donald T

    2014-01-01

    Graphics processing units are increasingly being used for scientific computing for their powerful parallel processing abilities, and moderate price compared to super computers and computing grids. In this paper we have used a general purpose graphics processing unit to process adaptive-optics optical coherence tomography (AOOCT) images in real time. Increasing the processing speed of AOOCT is an essential step in moving the super high resolution technology closer to clinical viability.

  19. Imaging optical fields below metal films and metal-dielectric waveguides by a scanning microscope

    Science.gov (United States)

    Zhu, Liangfu; Wang, Yong; Zhang, Douguo; Wang, Ruxue; Qiu, Dong; Wang, Pei; Ming, Hai; Badugu, Ramachandram; Rosenfeld, Mary; Lakowicz, Joseph R.

    2017-09-01

    Laser scanning confocal fluorescence microscopy (LSCM) is now an important method for tissue and cell imaging when the samples are located on the surfaces of glass slides. In the past decade, there has been extensive development of nano-optical structures that display unique effects on incident and transmitted light, which will be used with novel configurations for medical and consumer products. For these applications, it is necessary to characterize the light distribution within short distances from the structures for efficient detection and elimination of bulky optical components. These devices will minimize or possibly eliminate the need for free-space light propagation outside of the device itself. We describe the use of the scanning function of a LSCM to obtain 3D images of the light intensities below the surface of nano-optical structures. More specifically, we image the spatial distributions inside the substrate of fluorescence emission coupled to waveguide modes after it leaks through thin metal films or dielectric-coated metal films. The observed spatial distribution were in general agreement with far-field calculations, but the scanning images also revealed light intensities at angles not observed with classical back focal plane imaging. Knowledge of the subsurface optical intensities will be crucial in the combination of nano-optical structures with rapidly evolving imaging detectors.

  20. Scanning laser optical computed tomography system for large volume 3D dosimetry

    Science.gov (United States)

    Dekker, Kurtis H.; Battista, Jerry J.; Jordan, Kevin J.

    2017-04-01

    Stray light causes artifacts in optical computed tomography (CT) that negatively affect the accuracy of radiation dosimetry in gels or solids. Scatter effects are exacerbated by a large dosimeter volume, which is desirable for direct verification of modern radiotherapy treatment plans such as multiple-isocenter radiosurgery. The goal in this study was to design and characterize an optical CT system that achieves high accuracy primary transmission measurements through effective stray light rejection, while maintaining sufficient scan speed for practical application. We present an optical imaging platform that uses a galvanometer mirror for horizontal scanning, and a translation stage for vertical movement of a laser beam and small area detector for minimal stray light production and acceptance. This is coupled with a custom lens-shaped optical CT aquarium for parallel ray sampling of projections. The scanner images 15 cm diameter, 12 cm height cylindrical volumes at 0.33 mm resolution in approximately 30 min. Attenuation coefficients reconstructed from CT scans agreed with independent cuvette measurements within 2% for both absorbing and scattering solutions as well as small 1.25 cm diameter absorbing phantoms placed within a large, scattering medium that mimics gel. Excellent linearity between the optical CT scanner and the independent measurement was observed for solutions with between 90% and 2% transmission. These results indicate that the scanner should achieve highly accurate dosimetry of large volume dosimeters in a reasonable timeframe for clinical application to radiotherapy dose verification procedures.

  1. Adaptive optics with pupil tracking for high resolution retinal imaging.

    Science.gov (United States)

    Sahin, Betul; Lamory, Barbara; Levecq, Xavier; Harms, Fabrice; Dainty, Chris

    2012-02-01

    Adaptive optics, when integrated into retinal imaging systems, compensates for rapidly changing ocular aberrations in real time and results in improved high resolution images that reveal the photoreceptor mosaic. Imaging the retina at high resolution has numerous potential medical applications, and yet for the development of commercial products that can be used in the clinic, the complexity and high cost of the present research systems have to be addressed. We present a new method to control the deformable mirror in real time based on pupil tracking measurements which uses the default camera for the alignment of the eye in the retinal imaging system and requires no extra cost or hardware. We also present the first experiments done with a compact adaptive optics flood illumination fundus camera where it was possible to compensate for the higher order aberrations of a moving model eye and in vivo in real time based on pupil tracking measurements, without the real time contribution of a wavefront sensor. As an outcome of this research, we showed that pupil tracking can be effectively used as a low cost and practical adaptive optics tool for high resolution retinal imaging because eye movements constitute an important part of the ocular wavefront dynamics.

  2. Accuracy of optical scanning methods of the Cerec®3D system in the process of making ceramic inlays

    Directory of Open Access Journals (Sweden)

    Trifković Branka

    2010-01-01

    Full Text Available Background/Aim. One of the results of many years of Cerec® 3D CAD/CAM system technological development is implementation of one intraoral and two extraoral optical scanning methods which, depending on the current indications, are applied in making fixed restorations. The aim of this study was to determine the degree of precision of optical scanning methods by the use of the Cerec®3D CAD/CAM system in the process of making ceramic inlays. Methods. The study was conducted in three experimental groups of inlays prepared using the procedure of three methods of scanning Cerec ®3D system. Ceramic inlays made by conventional methodology were the control group. The accuracy of optical scanning methods of the Cerec®3D system computer aided designcomputer aided manufacturing (CAD/CAM was indirectly examined by measuring a marginal gap size between inlays and demarcation preparation by scanning electron microscope (SEM. Results. The results of the study showed a difference in the accuracy of the existing methods of scanning dental CAD/CAM systems. The highest level of accuracy was achieved by the extraoral optical superficial scanning technique. The value of marginal gap size inlays made with the technique of extraoral optical superficial scanning was 32.97 ± 13.17 μ. Techniques of intraoral optical superficial and extraoral point laser scanning showed a lower level of accuracy (40.29 ± 21.46 μ for inlays of intraoral optical superficial scanning and 99.67 ± 37.25 μ for inlays of extraoral point laser scanning. Conclusion. Optical scanning methods in dental CAM/CAM technologies are precise methods of digitizing the spatial models; application of extraoral optical scanning methods provides the hightest precision.

  3. Sub-nanosecond time-resolved near-field scanning magneto-optical microscope.

    Science.gov (United States)

    Rudge, J; Xu, H; Kolthammer, J; Hong, Y K; Choi, B C

    2015-02-01

    We report on the development of a new magnetic microscope, time-resolved near-field scanning magneto-optical microscope, which combines a near-field scanning optical microscope and magneto-optical contrast. By taking advantage of the high temporal resolution of time-resolved Kerr microscope and the sub-wavelength spatial resolution of a near-field microscope, we achieved a temporal resolution of ∼50 ps and a spatial resolution of microscope, the magnetic field pulse induced gyrotropic vortex dynamics occurring in 1 μm diameter, 20 nm thick CoFeB circular disks has been investigated. The microscope provides sub-wavelength resolution magnetic images of the gyrotropic motion of the vortex core at a resonance frequency of ∼240 MHz.

  4. Tip-enhanced near-field Raman spectroscopy with a scanning tunneling microscope and side-illumination optics.

    Science.gov (United States)

    Yi, K J; He, X N; Zhou, Y S; Xiong, W; Lu, Y F

    2008-07-01

    Conventional Raman spectroscopy (RS) suffers from low spatial resolution and low detection sensitivity due to the optical diffraction limit and small interaction cross sections. It has been reported that a highly localized and significantly enhanced electromagnetic field could be generated in the proximity of a metallic tip illuminated by a laser beam. In this study, a tip-enhanced RS system was developed to both improve the resolution and enhance the detection sensitivity using the tip-enhanced near-field effects. This instrument, by combining RS with a scanning tunneling microscope and side-illumination optics, demonstrated significant enhancement on both optical sensitivity and spatial resolution using either silver (Ag)-coated tungsten (W) tips or gold (Au) tips. The sensitivity improvement was verified by observing the enhancement effects on silicon (Si) substrates. Lateral resolution was verified to be below 100 nm by mapping Ag nanostructures. By deploying the depolarization technique, an apparent enhancement of 175% on Si substrates was achieved. Furthermore, the developed instrument features fast and reliable optical alignment, versatile sample adaptability, and effective suppression of far-field signals.

  5. Adaptation of scanning saccades co-occurs in different coordinate systems.

    Science.gov (United States)

    Lévy-Bencheton, Delphine; Pélisson, Denis; Panouillères, Muriel; Urquizar, Christian; Tilikete, Caroline; Pisella, Laure

    2014-06-15

    Plastic changes of saccades (i.e., following saccadic adaptation) do not transfer between oppositely directed saccades, except when multiple directions are trained simultaneously, suggesting a saccadic planning in retinotopic coordinates. Interestingly, a recent study in human healthy subjects revealed that after an adaptive increase of rightward-scanning saccades, both leftward and rightward double-step, memory-guided saccades, triggered toward the adapted endpoint, were modified, revealing that target location was coded in spatial coordinates (Zimmermann et al. 2011). However, as the computer screen provided a visual frame, one alternative hypothesis could be a coding in allocentric coordinates. Here, we questioned whether adaptive modifications of saccadic planning occur in multiple coordinate systems. We reproduced the paradigm of Zimmermann et al. (2011) using target light-emitting diodes in the dark, with and without a visual frame, and tested different saccades before and after adaptation. With double-step, memory-guided saccades, we reproduced the transfer of adaptation to leftward saccades with the visual frame but not without, suggesting that the coordinate system used for saccade planning, when the frame is visible, is allocentric rather than spatiotopic. With single-step, memory-guided saccades, adaptation transferred to leftward saccades, both with and without the visual frame, revealing a target localization in a coordinate system that is neither retinotopic nor allocentric. Finally, with single-step, visually guided saccades, the classical, unidirectional pattern of amplitude change was reproduced, revealing retinotopic coordinate coding. These experiments indicate that the same procedure of adaptation modifies saccadic planning in multiple coordinate systems in parallel-each of them revealed by the use of different saccade tasks in postadaptation. Copyright © 2014 the American Physiological Society.

  6. High-resolution retinal imaging using adaptive optics and Fourier-domain optical coherence tomography

    Science.gov (United States)

    Olivier, Scot S.; Werner, John S.; Zawadzki, Robert J.; Laut, Sophie P.; Jones, Steven M.

    2010-09-07

    This invention permits retinal images to be acquired at high speed and with unprecedented resolution in three dimensions (4.times.4.times.6 .mu.m). The instrument achieves high lateral resolution by using adaptive optics to correct optical aberrations of the human eye in real time. High axial resolution and high speed are made possible by the use of Fourier-domain optical coherence tomography. Using this system, we have demonstrated the ability to image microscopic blood vessels and the cone photoreceptor mosaic.

  7. The optical alignment of the Gemini planet imager adaptive optics bench

    Science.gov (United States)

    Pazder, John; Bauman, Brian; Dillon, Daren; Fletcher, Murray; Lacoursière, Jean; Reshetov, Vlad

    2012-09-01

    The Gemini Planet Imager (GPI) is a facility instrument under construction for the 8-m Gemini South telescope. This paper describes the methods used for optical alignment of the adaptive optics (AO) bench. The optical alignment of the off-axis paraboloid mirrors was done using a pre-alignment method utilizing a HeNe laser and alignment telescopes followed by a fine-tuning using a Shack-Hartmann wavefront sensor and a shear plate. A FARO arm measuring system was used to place the fiducials for the alignment. Using these methods the AO bench was aligned to 13nm RMS of wavefront error.

  8. Correcting the aero-optical aberration of the supersonic mixing layer with adaptive optics: concept validation.

    Science.gov (United States)

    Gao, Qiong; Jiang, Zongfu; Yi, Shihe; Xie, Wenke; Liao, Tianhe

    2012-06-10

    We describe an adaptive optics (AO) system for correcting the aero-optical aberration of the supersonic mixing layer and test its performance with numerical simulations. The AO system is based on the measurement of distributed Strehl ratios and the stochastic parallel gradient descent (SPGD) algorithm. The aero-optical aberration is computed by the direct numerical simulation of a two-dimensional supersonic mixing layer. When the SPGD algorithm is applied directly, the AO cannot give effective corrections. This paper suggests two strategies to improve the performance of the SPGD algorithm for use in aero-optics. The first one is using an iteration process keeping finite memory, and the second is based on the frozen hypothesis. With these modifications, the performance of AO is improved and the aero-optical aberration can be corrected to some noticeable extent. The possibility of experimental implementation is also discussed.

  9. Optical coherence tomography, scanning laser polarimetry and confocal scanning laser ophthalmoscopy in retinal nerve fiber layer measurements of glaucoma patients.

    Science.gov (United States)

    Fanihagh, Farsad; Kremmer, Stephan; Anastassiou, Gerasimos; Schallenberg, Maurice

    2015-01-01

    To determine the correlations and strength of association between different imaging systems in analyzing the retinal nerve fiber layer (RNFL) of glaucoma patients: optical coherence tomography (OCT), scanning laser polarimetry (SLP) and confocal scanning laser ophthalmoscopy (CSLO). 114 eyes of patients with moderate open angle glaucoma underwent spectral domain OCT (Topcon SD-OCT 2000 and Zeiss Cirrus HD-OCT), SLP (GDx VCC and GDx Pro) and CSLO (Heidelberg Retina Tomograph, HRT 3). Correlation coefficients were calculated between the structural parameters yielded by these examinations. The quantitative relationship between the measured RNFL thickness globally and for the four regions (superior, inferior, nasal, temporal) were evaluated with different regression models for all used imaging systems. The strongest correlation of RNFL measurements was found between devices using the same technology like GDx VCC and GDx Pro as well as Topcon OCT and Cirrus OCT. In glaucoma patients, the strongest associations (R²) were found between RNFL measurements of the two optical coherence tomography devices Topcon OCT and Cirrus OCT (R² = 0.513) and between GDx VCC and GDx Pro (R² = 0.451). The results of the OCTs and GDX Pro also had a strong quantitative relationship (Topcon OCT R² = 0.339 and Cirrus OCT R² = 0.347). GDx VCC and the OCTs showed a mild to moderate association (Topcon OCT R² = 0.207 and Cirrus OCT R² = 0.258). The confocal scanning laser ophthalmoscopy (HRT 3) had the lowest association to all other devices (Topcon OCT R² = 0.254, Cirrus OCT R² = 0.158, GDx Pro R² = 0.086 and GDx VCC R² = 0.1). The measurements of the RNFL in glaucoma patients reveal a high correlation of OCT and GDx devices because OCTs can measure all major retinal layers and SLP can detect nerve fibers allowing a comparison between the results of this devices. However, CSLO by means of HRT topography can only measure height values of the retinal surface but it cannot distinguish

  10. Handheld ultrahigh speed swept source optical coherence tomography instrument using a MEMS scanning mirror.

    Science.gov (United States)

    Lu, Chen D; Kraus, Martin F; Potsaid, Benjamin; Liu, Jonathan J; Choi, Woojhon; Jayaraman, Vijaysekhar; Cable, Alex E; Hornegger, Joachim; Duker, Jay S; Fujimoto, James G

    2013-12-20

    We developed an ultrahigh speed, handheld swept source optical coherence tomography (SS-OCT) ophthalmic instrument using a 2D MEMS mirror. A vertical cavity surface-emitting laser (VCSEL) operating at 1060 nm center wavelength yielded a 350 kHz axial scan rate and 10 µm axial resolution in tissue. The long coherence length of the VCSEL enabled a 3.08 mm imaging range with minimal sensitivity roll-off in tissue. Two different designs with identical optical components were tested to evaluate handheld OCT ergonomics. An iris camera aided in alignment of the OCT beam through the pupil and a manual fixation light selected the imaging region on the retina. Volumetric and high definition scans were obtained from 5 undilated normal subjects. Volumetric OCT data was acquired by scanning the 2.4 mm diameter 2D MEMS mirror sinusoidally in the fast direction and linearly in the orthogonal slow direction. A second volumetric sinusoidal scan was obtained in the orthogonal direction and the two volumes were processed with a software algorithm to generate a merged motion-corrected volume. Motion-corrected standard 6 x 6 mm(2) and wide field 10 x 10 mm(2) volumetric OCT data were generated using two volumetric scans, each obtained in 1.4 seconds. High definition 10 mm and 6 mm B-scans were obtained by averaging and registering 25 B-scans obtained over the same position in 0.57 seconds. One of the advantages of volumetric OCT data is the generation of en face OCT images with arbitrary cross sectional B-scans registered to fundus features. This technology should enable screening applications to identify early retinal disease, before irreversible vision impairment or loss occurs. Handheld OCT technology also promises to enable applications in a wide range of settings outside of the traditional ophthalmology or optometry clinics including pediatrics, intraoperative, primary care, developing countries, and military medicine.

  11. Handheld ultrahigh speed swept source optical coherence tomography instrument using a MEMS scanning mirror

    Science.gov (United States)

    Lu, Chen D.; Kraus, Martin F.; Potsaid, Benjamin; Liu, Jonathan J.; Choi, WooJhon; Jayaraman, Vijaysekhar; Cable, Alex E.; Hornegger, Joachim; Duker, Jay S.; Fujimoto, James G.

    2013-01-01

    We developed an ultrahigh speed, handheld swept source optical coherence tomography (SS-OCT) ophthalmic instrument using a 2D MEMS mirror. A vertical cavity surface-emitting laser (VCSEL) operating at 1060 nm center wavelength yielded a 350 kHz axial scan rate and 10 µm axial resolution in tissue. The long coherence length of the VCSEL enabled a 3.08 mm imaging range with minimal sensitivity roll-off in tissue. Two different designs with identical optical components were tested to evaluate handheld OCT ergonomics. An iris camera aided in alignment of the OCT beam through the pupil and a manual fixation light selected the imaging region on the retina. Volumetric and high definition scans were obtained from 5 undilated normal subjects. Volumetric OCT data was acquired by scanning the 2.4 mm diameter 2D MEMS mirror sinusoidally in the fast direction and linearly in the orthogonal slow direction. A second volumetric sinusoidal scan was obtained in the orthogonal direction and the two volumes were processed with a software algorithm to generate a merged motion-corrected volume. Motion-corrected standard 6 x 6 mm2 and wide field 10 x 10 mm2 volumetric OCT data were generated using two volumetric scans, each obtained in 1.4 seconds. High definition 10 mm and 6 mm B-scans were obtained by averaging and registering 25 B-scans obtained over the same position in 0.57 seconds. One of the advantages of volumetric OCT data is the generation of en face OCT images with arbitrary cross sectional B-scans registered to fundus features. This technology should enable screening applications to identify early retinal disease, before irreversible vision impairment or loss occurs. Handheld OCT technology also promises to enable applications in a wide range of settings outside of the traditional ophthalmology or optometry clinics including pediatrics, intraoperative, primary care, developing countries, and military medicine. PMID:24466495

  12. Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy

    Science.gov (United States)

    Hunter, Jennifer J.; Masella, Benjamin; Dubra, Alfredo; Sharma, Robin; Yin, Lu; Merigan, William H.; Palczewska, Grazyna; Palczewski, Krzysztof; Williams, David R.

    2011-01-01

    In vivo two-photon imaging through the pupil of the primate eye has the potential to become a useful tool for functional imaging of the retina. Two-photon excited fluorescence images of the macaque cone mosaic were obtained using a fluorescence adaptive optics scanning laser ophthalmoscope, overcoming the challenges of a low numerical aperture, imperfect optics of the eye, high required light levels, and eye motion. Although the specific fluorophores are as yet unknown, strong in vivo intrinsic fluorescence allowed images of the cone mosaic. Imaging intact ex vivo retina revealed that the strongest two-photon excited fluorescence signal comes from the cone inner segments. The fluorescence response increased following light stimulation, which could provide a functional measure of the effects of light on photoreceptors. PMID:21326644

  13. Deformation of multilayers and optical surfaces in soft x-ray adaptive optics (Conference Presentation)

    Science.gov (United States)

    Wylie-van Eerd, Benjamin J.; Yuan, Huiyu; Houwman, Evert; Antonov, Oleksandr; Louis, Eric; Yakshin, Andrey E.; Rijnders, Guus J. H. M.; Bijkerk, Fred

    2016-09-01

    Adaptive optics are of great utility in improving the resolving power of imaging and projection systems. In EUV lithography systems, for example, an adaptive optic can correct for wavefront deformation and decrease the feature size of integrated circuits that the system is in practice able to print. Piezoelectric thin films can be shown to accurately deform their surface with the sub-Angstrom precision required in order to compensate for wavefront deformation in EUV lithographic systems. However, in order to develop this from concept to working device, reflective coatings must be grown on top of the piezoelectric layer. Normal incidence EUV adaptive optics must meet the challenge of manipulating multilayer reflective coatings, while simultaneously preserving over many cycles the finely tuned structural properties that result in high XUV reflectivity. At this moment there are many unanswered questions in the literature about the behavior of an EUV multilayer under strain and the interaction of piezoelectric elements with multilayers. In this talk, we will present modelling of the response of multilayers to inhomogeneous strains that may be expected in a normal-incidence EUV adaptive optic, and preliminary experimental results.

  14. Adaptive and robust statistical methods for processing near-field scanning microwave microscopy images.

    Science.gov (United States)

    Coakley, K J; Imtiaz, A; Wallis, T M; Weber, J C; Berweger, S; Kabos, P

    2015-03-01

    Near-field scanning microwave microscopy offers great potential to facilitate characterization, development and modeling of materials. By acquiring microwave images at multiple frequencies and amplitudes (along with the other modalities) one can study material and device physics at different lateral and depth scales. Images are typically noisy and contaminated by artifacts that can vary from scan line to scan line and planar-like trends due to sample tilt errors. Here, we level images based on an estimate of a smooth 2-d trend determined with a robust implementation of a local regression method. In this robust approach, features and outliers which are not due to the trend are automatically downweighted. We denoise images with the Adaptive Weights Smoothing method. This method smooths out additive noise while preserving edge-like features in images. We demonstrate the feasibility of our methods on topography images and microwave |S11| images. For one challenging test case, we demonstrate that our method outperforms alternative methods from the scanning probe microscopy data analysis software package Gwyddion. Our methods should be useful for massive image data sets where manual selection of landmarks or image subsets by a user is impractical. Published by Elsevier B.V.

  15. Dual-scanning optical coherence elastography for rapid imaging of two tissue volumes (Conference Presentation)

    Science.gov (United States)

    Fang, Qi; Frewer, Luke; Wijesinghe, Philip; Hamzah, Juliana; Ganss, Ruth; Allen, Wes M.; Sampson, David D.; Curatolo, Andrea; Kennedy, Brendan F.

    2017-02-01

    In many applications of optical coherence elastography (OCE), it is necessary to rapidly acquire images in vivo, or within intraoperative timeframes, over fields-of-view far greater than can be achieved in one OCT image acquisition. For example, tumour margin assessment in breast cancer requires acquisition over linear dimensions of 4-5 centimetres in under 20 minutes. However, the majority of existing techniques are not compatible with these requirements, which may present a hurdle to the effective translation of OCE. To increase throughput, we have designed and developed an OCE system that simultaneously captures two 3D elastograms from opposite sides of a sample. The optical system comprises two interferometers: a common-path interferometer on one side of the sample and a dual-arm interferometer on the other side. This optical system is combined with scanning mechanisms and compression loading techniques to realize dual-scanning OCE. The optical signals scattered from two volumes are simultaneously detected on a single spectrometer by depth-encoding the interference signal from each interferometer. To demonstrate dual-scanning OCE, we performed measurements on tissue-mimicking phantoms containing rigid inclusions and freshly isolated samples of murine hepatocellular carcinoma, highlighting the use of this technique to visualise 3D tumour stiffness. These findings indicate that our technique holds promise for in vivo and intraoperative applications.

  16. Foundry Microfabrication of Deformable Mirrors for Adaptive Optics

    Science.gov (United States)

    1998-04-28

    and may be indicative of metal creep over time. Figure 4-2. Representative interferometric microscope scan measurement of gold/Poly2 micromirror...with this prediction, indicating that metal creep may occur over time. 4.6 Mirror Designs for Optical Testing In an effort to examine the effects of...57 6 5 118 B10 123 12 5 6 C1 58 6 6 94 G14 124 12 6 11 E1 59 6 7 60 N10 125 12 7 12 G2 60 6 8 59 P10 126 12 8 28 N1 61 6 9 58 P9 127 12 9 33 M3

  17. Scanning a DNA molecule for bound proteins using hybrid magnetic and optical tweezers.

    Directory of Open Access Journals (Sweden)

    Marijn T J van Loenhout

    Full Text Available The functional state of the genome is determined by its interactions with proteins that bind, modify, and move along the DNA. To determine the positions and binding strength of proteins localized on DNA we have developed a combined magnetic and optical tweezers apparatus that allows for both sensitive and label-free detection. A DNA loop, that acts as a scanning probe, is created by looping an optically trapped DNA tether around a DNA molecule that is held with magnetic tweezers. Upon scanning the loop along the λ-DNA molecule, EcoRI proteins were detected with ~17 nm spatial resolution. An offset of 33 ± 5 nm for the detected protein positions was found between back and forwards scans, corresponding to the size of the DNA loop and in agreement with theoretical estimates. At higher applied stretching forces, the scanning loop was able to remove bound proteins from the DNA, showing that the method is in principle also capable of measuring the binding strength of proteins to DNA with a force resolution of 0.1 pN/[Formula: see text]. The use of magnetic tweezers in this assay allows the facile preparation of many single-molecule tethers, which can be scanned one after the other, while it also allows for direct control of the supercoiling state of the DNA molecule, making it uniquely suitable to address the effects of torque on protein-DNA interactions.

  18. Comparasion of Optic Nerve Head with Stereophotometric and Scanning Laser Ophthalmoscopic Imaging

    Directory of Open Access Journals (Sweden)

    Serek Tekin

    2016-01-01

    Full Text Available Aim: To compare theevaluation results of two experienced clinicians about examination of optic discs in glaucoma patients and healthy inidividuals by stereophotometry and scanning laser ophthalmoscopy. Material and Method: We studied 116 individuals (217 eyes who were divided as normal, glaucoma and suspected glaucoma in numbers of 54, 42 and 20 respectively. Stereophotometric photographs of optic disc were examined with fundus camera (Zeiss, FF 450 plus. Optic disc was also evaluated with HRT-3 in the same visit. Two experienced clinicians evaluated the cup/disc ratios and whether the optic discs were glaucomatous or not. Evaluation results were analysed and compared with HRT-3 examinations. Results:There were no significant age and gende rdifferences between the groups(p>0.05.Stereophotographic C/D ratio correlations between the clinicians were 0.79 (p

  19. Real-time phase error compensation in phase sensitive scanning near-field optical microscopy.

    Science.gov (United States)

    Wu, Xiaoyu; Sun, Lin; Wang, Jia; Tan, Qiaofeng

    2015-07-01

    Phase measurements are critical for investigations on the optical properties of surface plasmon polariton (SPP) nanostructures. In this paper, a real-time phase error compensation method based on a phase sensitive scanning near-field optical microscopy (SNOM) measurement system is proposed. The method adopts the common optical path configuration and CMR (common-mode rejection) principle. It can be seen that the phase error compensation is real-time and mainly relies on optical devices, therefore neither post processing nor previous knowledge of environmental effects is required. The causes of the phase drift errors are discussed. We demonstrate experimentally the effectiveness of this method by measuring a SPP focusing device. Regardless of the drift velocity, degree of linearity, or phase accuracy, the compensation method shows great improvement compared to the previous phase sensitive SNOMs. All the measured distributions are in good agreement with theoretical simulations obtained by the finite-different time-domain (FDTD) method.

  20. Graphite/Cyanate Ester Face Sheets for Adaptive Optics

    Science.gov (United States)

    Bennett, Harold; Shaffer, Joseph; Romeo, Robert

    2008-01-01

    It has been proposed that thin face sheets of wide-aperture deformable mirrors in adaptive-optics systems be made from a composite material consisting of cyanate ester filled with graphite. This composite material appears to offer an attractive alternative to low-thermal-expansion glasses that are used in some conventional optics and have been considered for adaptive-optics face sheets. Adaptive-optics face sheets are required to have maximum linear dimensions of the order of meters or even tens of meters for some astronomical applications. If the face sheets were to be made from low-thermal-expansion glasses, then they would also be required to have thicknesses of the order of a millimeter so as to obtain the optimum compromise between the stiffness needed for support and the flexibility needed to enable deformation to controlled shapes by use of actuators. It is difficult to make large glass sheets having thicknesses less than 3 mm, and 3-mm-thick glass sheets are too stiff to be deformable to the shapes typically required for correction of wavefronts of light that has traversed the terrestrial atmosphere. Moreover, the primary commercially produced candidate low-thermal-expansion glass is easily fractured when in the form of thin face sheets. Graphite-filled cyanate ester has relevant properties similar to those of the low-expansion glasses. These properties include a coefficient of thermal expansion (CTE) of the order of a hundredth of the CTEs of other typical mirror materials. The Young s modulus (which quantifies stiffness in tension and compression) of graphite-filled cyanate ester is also similar to the Young's moduli of low-thermal-expansion glasses. However, the fracture toughness of graphite-filled cyanate ester is much greater than that of the primary candidate low-thermal-expansion glass. Therefore, graphite-filled cyanate ester could be made into nearly unbreakable face sheets, having maximum linear dimensions greater than a meter and thicknesses of

  1. Low Temperature Apertureless Near-field Scanning Optical Microscope for Optical Spectroscopy of Single Ge/Si Quantum Dots

    Science.gov (United States)

    Zhu, Henry; Patil, N. G.; Levy, Jeremy

    2001-03-01

    A low-temperature apertureless near-field scanning optical microscope has been designed and constructed for the purpose of investigating the optical properties of individual Ge/Si quantum dots. The microscope fits in the 37 mm bore of a Helium vapor magneto-optic cryostat, allowing operations down to liquid helium temperatures in magnetic fields up to 8 Tesla. An in situ microscope objective focuses light onto the sample, which is scanned in the three spatial directions using a compact modular stage. An AFM/STM tip resides on the top; feedback is achieved using a quartz tuning fork oscillator. Both tip and objective are attached to inertial sliding motors that can move in fine (10 nm) steps to achieve touchdown and focus. A femtosecond optical parametric oscillator is used to excite carriers in the quantum dots both resonantly and non-resonantly; scattered luminescence from the AFM/STM tip is collected and analyzed spectrally using a 1/2 meter imaging spectrometer and a LN_2-cooled InGaAs array. We gratefully acknowledge NSF (DMR-9701725, IMR-9802784) and DARPA (DAAD-16-99-C1036) for financial support of this work.

  2. Analysis of light scattering from human breast tissue using a custom dual-optical scanning near-field optical microscope.

    Science.gov (United States)

    Kyle, Jennifer Reiber; Kyle, Michael D; Raghavan, Ravi; Budak, Gurer; Ozkan, Cengiz S; Ozkan, Mihrimah

    2011-03-01

    In this paper we introduce a custom scanning near-field optical microscope (SNOM) that simultaneously collects reflection and transmission near-field images along with topography. This dual-optical SNOM uses a bent probe, which allows for axial reflection imaging, accurate surface scanning, and easy identification of topographic artifacts. Using this novel dual-optical SNOM, we image desiccated and non-desiccated human breast epithelial tissue. By comparing the simultaneous SNOM images, we isolate the effects of tissue morphology and variations in refractive indices on the forward- and back-scattering of light from the tissue. We find that the reduction in back-scattering from tissue, relative to the glass slide, is caused by dense packing of the scattering sites in the cytoplasm (morphology) in the desiccated tissue and a thin-film of water adhering to the glass slide (refractive index) in the non-desiccated tissue sample. Our work demonstrates the potential of our customized dual-optical SNOM system for label-free tissue diagnostics. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Factors Affecting Cirrus-HD OCT Optic Disc Scan Quality: A Review with Case Examples

    Directory of Open Access Journals (Sweden)

    Joshua S. Hardin

    2015-01-01

    Full Text Available Spectral-domain OCT is an established tool to assist clinicians in detecting glaucoma and monitor disease progression. The widespread use of this imaging modality is due, at least in part, to continuous hardware and software advancements. However, recent evidence indicates that OCT scan artifacts are frequently encountered in clinical practice. Poor image quality invariably challenges the interpretation of test results, with potential implications for the care of glaucoma patients. Therefore, adequate knowledge of various imaging artifacts is necessary. In this work, we describe several factors affecting Cirrus HD-OCT optic disc scan quality and their effects on measurement variability.

  4. Observation of mesenteric microcirculatory disturbance in rat by laser oblique scanning optical microscopy

    Science.gov (United States)

    Ding, Yichen; Zhang, Yu; Peng, Tong; Lu, Yiqing; Jin, Dayong; Ren, Qiushi; Liu, Yuying; Han, Jingyan; Xi, Peng

    2013-05-01

    Ischemia-reperfusion (I/R) injury model has been widely applied to the study of microcirculation disturbance. In this work, we used laser oblique scanning optical microscopy (LOSOM) to observe the microcirculation system in the mesentery of rat model. Utilizing a localized point-scanning detection scheme, high-contrast images of leukocytes were obtained. The extended detection capability facilitated both the automatic in vivo cell counting and the accurate measurement of the rolling velocity of leukocytes. Statistical analysis of the different treatment groups suggested that the distinction between I/R and sham groups with time lapse is significant.

  5. Adapting smartphones for low-cost optical medical imaging

    Science.gov (United States)

    Pratavieira, Sebastião.; Vollet-Filho, José D.; Carbinatto, Fernanda M.; Blanco, Kate; Inada, Natalia M.; Bagnato, Vanderlei S.; Kurachi, Cristina

    2015-06-01

    Optical images have been used in several medical situations to improve diagnosis of lesions or to monitor treatments. However, most systems employ expensive scientific (CCD or CMOS) cameras and need computers to display and save the images, usually resulting in a high final cost for the system. Additionally, this sort of apparatus operation usually becomes more complex, requiring more and more specialized technical knowledge from the operator. Currently, the number of people using smartphone-like devices with built-in high quality cameras is increasing, which might allow using such devices as an efficient, lower cost, portable imaging system for medical applications. Thus, we aim to develop methods of adaptation of those devices to optical medical imaging techniques, such as fluorescence. Particularly, smartphones covers were adapted to connect a smartphone-like device to widefield fluorescence imaging systems. These systems were used to detect lesions in different tissues, such as cervix and mouth/throat mucosa, and to monitor ALA-induced protoporphyrin-IX formation for photodynamic treatment of Cervical Intraepithelial Neoplasia. This approach may contribute significantly to low-cost, portable and simple clinical optical imaging collection.

  6. A pupil tracking system for adaptive optics retinal imaging

    Science.gov (United States)

    Sahin, Betul; Harms, Fabrice; Lamory, Barbara; Vabre, Laurent

    2008-04-01

    High-resolution imaging of the retina is a challenge due to the optical aberrations introduced by the eye, a living system in constant change and motion. Adaptive Optics (AO) is particularly suited to the continuous, dynamic correction of aberrations as they change over time. In particular, eye pupil displacements induce fast-changing wave front errors which lead to a need for faster wave front sensors. We propose a new approach for ocular adaptive optics by adding a Pupil Tracking System (PTS) into the AO loop. This system is different from the existing eye tracking devices by its speed, high precision in a short range and therefore its suitability for integration in an AO loop. Performance tests done using an artificial eye with a pupil diameter of 7 mm have shown promising results. These tests have demonstrated that the device achieves an accuracy of <15 μm in a +/-2 mm range of eye movements with a standard deviation <10 μm, and requires less than 12 ms for each detection.

  7. An inverse problem of estimating the heat source in tapered optical fibers for scanning near-field optical microscopy.

    Science.gov (United States)

    Lee, Haw-Long; Chang, Win-Jin; Chen, Wen-Lih; Yang, Yu-Ching

    2007-08-01

    A conjugate gradient method based on inverse algorithm is applied in this study to estimate the unknown space- and time-dependent heat source in aluminum-coated tapered optical fibers for scanning near-field optical microscopy, by reading the transient temperature data at the measurement positions. No prior information is available on the functional form of the unknown heat source in the present study; thus, it is classified as the function estimation in inverse calculation. The accuracy of the inverse analysis is examined by using the simulated exact and inexact temperature measurements. Results show that an excellent estimation on the heat source and temperature distributions in the tapered optical fiber can be obtained for all the test cases considered in this study.

  8. Application of adaptive optics for flexible laser induced ultrasound field generation and uncertainty reduction in measurements

    Science.gov (United States)

    Büttner, Lars; Schmieder, Felix; Teich, Martin; Koukourakis, Nektarios; Czarske, Jürgen

    2017-06-01

    The availability of spatial light modulators as standard turnkey components and their ongoing development makes them attractive for a huge variety of optical measurement systems in industry and research. Here, we outline two examples of how optical measurements can benefit from spatial light modulators. Ultrasound testing has become an indispensable tool for industrial inspection. Contact-free measurements can be achieved by laser-induced ultrasound. One disadvantage is that due to the highly divergent sound field of the generated shear waves for a point-wise thermoelastic excitation, only a poor spatial selectivity can be achieved. This problem can be solved by creating an ultrasound focus by means of a ring-like laser intensity distribution, but standard fixed-form optical components used for their generation are always optimised to a fixed set of parameters. Here, we demonstrate, how a predefined intensity pattern as e.g. a ring can be created from an arbitrary input laser beam using a phase-retrieval algorithm to shape an ultrasound focus in the sample. By displaying different patterns on the spatial light modulator, the focus can be traversed in all three directions through the object allowing a fast and highly spatially resolving scanning of the sample. Optical measurements take often place under difficult conditions. They are affected by variations of the refractive index, caused e.g. by phase boundaries between two media of different optical density. This will result in an increased measurement uncertainty or, in the worst case, will cause the measurement to fail. To overcome these limitations, we propose the application of adaptive optics. Optical flow velocity measurements based on image correlation in water that are performed through optical distortions are discussed. We demonstrate how the measurement error induced by refractive index variations can be reduced if a spatial light modulator is used in the measurement setup to compensate for the wavefront

  9. Z-scan and optical limiting properties of Hibiscus Sabdariffa dye

    Science.gov (United States)

    Diallo, A.; Zongo, S.; Mthunzi, P.; Rehman, S.; Alqaradawi, S. Y.; Soboyejo, W.; Maaza, M.

    2014-12-01

    The intensity-dependent refractive index n 2 and the nonlinear susceptibility χ (3) of Hibiscus Sabdariffa dye solutions in the nanosecond regime at 532 nm are reported. More presicely, the variation of n 2, β, and real and imaginary parts of χ (3) versus the natural dye extract concentration has been carried out by z-scan and optical limiting techniques. The third-order nonlinearity of the Hibiscus Sabdariffa dye solutions was found to be dominated by nonlinear refraction, which leads to strong optical limiting of laser.

  10. Volumetric display system based on three-dimensional scanning of inclined optical image.

    Science.gov (United States)

    Miyazaki, Daisuke; Shiba, Kensuke; Sotsuka, Koji; Matsushita, Kenji

    2006-12-25

    A volumetric display system based on three-dimensional (3D) scanning of an inclined image is reported. An optical image of a two-dimensional (2D) display, which is a vector-scan display monitor placed obliquely in an optical imaging system, is moved laterally by a galvanometric mirror scanner. Inclined cross-sectional images of a 3D object are displayed on the 2D display in accordance with the position of the image plane to form a 3D image. Three-dimensional images formed by this display system satisfy all the criteria for stereoscopic vision because they are real images formed in a 3D space. Experimental results of volumetric imaging from computed-tomography images and 3D animated images are presented.

  11. Quasi-4D laser diagnostics using an acousto-optic deflector scanning system

    Science.gov (United States)

    Li, Tao; Pareja, Jhon; Becker, Lukas; Heddrich, Wolfgang; Dreizler, Andreas; Böhm, Benjamin

    2017-03-01

    In this paper, a novel scanning system for laser diagnostics was developed and characterized. The system is based on the acousto-optic deflection of a high-speed pulsed laser. Results showed that quasi-volumetric laser illumination with high precision and accuracy can be achieved with a simplified and flexible optical setup. The feasibility of the method for performing high-speed quasi-4D laser diagnostics was demonstrated by the tomographic visualization of a lifted turbulent jet flame using Mie-scattering and multi-plane particle image velocimetry measurements of a turbulent non-reactive mixing case. Three-dimensional flame and flow structures can be detected and tracked with this new scanning system.

  12. Fourier transform digital holographic adaptive optics imaging system

    Science.gov (United States)

    Liu, Changgeng; Yu, Xiao; Kim, Myung K.

    2013-01-01

    A Fourier transform digital holographic adaptive optics imaging system and its basic principles are proposed. The CCD is put at the exact Fourier transform plane of the pupil of the eye lens. The spherical curvature introduced by the optics except the eye lens itself is eliminated. The CCD is also at image plane of the target. The point-spread function of the system is directly recorded, making it easier to determine the correct guide-star hologram. Also, the light signal will be stronger at the CCD, especially for phase-aberration sensing. Numerical propagation is avoided. The sensor aperture has nothing to do with the resolution and the possibility of using low coherence or incoherent illumination is opened. The system becomes more efficient and flexible. Although it is intended for ophthalmic use, it also shows potential application in microscopy. The robustness and feasibility of this compact system are demonstrated by simulations and experiments using scattering objects. PMID:23262541

  13. Aberrations and adaptive optics in super-resolution microscopy

    Science.gov (United States)

    Booth, Martin; Andrade, Débora; Burke, Daniel; Patton, Brian; Zurauskas, Mantas

    2015-01-01

    As one of the most powerful tools in the biological investigation of cellular structures and dynamic processes, fluorescence microscopy has undergone extraordinary developments in the past decades. The advent of super-resolution techniques has enabled fluorescence microscopy – or rather nanoscopy – to achieve nanoscale resolution in living specimens and unravelled the interior of cells with unprecedented detail. The methods employed in this expanding field of microscopy, however, are especially prone to the detrimental effects of optical aberrations. In this review, we discuss how super-resolution microscopy techniques based upon single-molecule switching, stimulated emission depletion and structured illumination each suffer from aberrations in different ways that are dependent upon intrinsic technical aspects. We discuss the use of adaptive optics as an effective means to overcome this problem. PMID:26124194

  14. Six-channel adaptive fibre-optic interferometer

    Energy Technology Data Exchange (ETDEWEB)

    Romashko, R V; Bezruk, M N; Kamshilin, A A; Kulchin, Yurii N

    2012-06-30

    We have proposed and analysed a scheme for the multiplexing of orthogonal dynamic holograms in photorefractive crystals which ensures almost zero cross talk between the holographic channels upon phase demodulation. A six-channel adaptive fibre-optic interferometer was built, and the detection limit for small phase fluctuations in the channels of the interferometer was determined to be 2.1 Multiplication-Sign 10{sup -8} rad W{sup 1/2} Hz{sup -1/2}. The channel multiplexing capacity of the interferometer was estimated. The formation of 70 channels such that their optical fields completely overlap in the crystal reduces the relative detection limit in the working channel by just 10 %. We found conditions under which the maximum cross talk between the channels was within the intrinsic noise level in the channels (-47 dB).

  15. Adaptive Optics Imaging of a Double Stellar Occultation by Titan

    Science.gov (United States)

    Bouchez, A. H.; West, R. A.; Brown, M. E.; Troy, M.; Burrus, R. S.; Dekany, R. G.

    2002-09-01

    We present resolved images of the occultation of a binary star by Titan, recorded with the Palomar Observatory adaptive optics system on 20 December 2001 UT. These constitute the first resolved observations of a stellar occultation by a small body, and demonstrate many unique phenomena not previously observed. Multiple refracted stellar images are visible on Titan's limb throughout both events, and precise astrometry has allowed us to use these to map the oblateness of Titan's atmosphere at the millibar level. Furthermore, the haze optical depth at two altitudes over each pole of Titan is sampled by the refracted starlight, allowing a unique comparison of the haze structure above the summer and winter poles to be made. We will present a movie of the imaging sequence.

  16. Adaptation of zirconia crowns created by conventional versus optical impression: in vitro study

    Science.gov (United States)

    Bahrami, Babak; Fossoyeux, InÈs; Atash, Ramin

    2017-01-01

    PURPOSE The aim of this study was to compare the precision of optical impression (Trios, 3Shape) versus that of conventional impression (Imprint IV, 3M-ESPE) with three different margins (shoulder, chamfer, and knife-edge) on Frasaco teeth. MATERIALS AND METHODS The sample comprised of 60 zirconia half-crowns, divided into six groups according to the type of impression and margin. Scanning electron microscopy enabled us to analyze the gap between the zirconia crowns and the Frasaco teeth, using ImageJ software, based on eight reproducible and standardized measuring points. RESULTS No statistically significant difference was found between conventional impressions and optical impressions, except for two of the eight points. A statistically significant difference was observed between the three margin types; the chamfer and knife-edge finishing lines appeared to offer better adaptation results than the shoulder margin. CONCLUSION Zirconia crowns created from optical impression and those created from conventional impression present similar adaptation. While offering identical results, the former have many advantages. In view of our findings, we believe the chamfer margin should be favored. PMID:28680553

  17. Self-characterization of linear and nonlinear adaptive optics systems.

    Science.gov (United States)

    Hampton, Peter J; Conan, Rodolphe; Keskin, Onur; Bradley, Colin; Agathoklis, Pan

    2008-01-10

    We present methods used to determine the linear or nonlinear static response and the linear dynamic response of an adaptive optics (AO) system. This AO system consists of a nonlinear microelectromechanical systems deformable mirror (DM), a linear tip-tilt mirror (TTM), a control computer, and a Shack-Hartmann wavefront sensor. The system is modeled using a single-input-single-output structure to determine the one-dimensional transfer function of the dynamic response of the chain of system hardware. An AO system has been shown to be able to characterize its own response without additional instrumentation. Experimentally determined models are given for a TTM and a DM.

  18. High resolution observations using adaptive optics: Achievements and future needs

    Science.gov (United States)

    Sankarasubramanian, K.; Rimmele, T.

    2008-06-01

    Over the last few years, several interesting observations were obtained with the help of solar Adaptive Optics (AO). In this paper, few observations made using the solar AO are enlightened and briefly discussed. A list of disadvantages with the current AO system are presented. With telescopes larger than 1.5 m expected during the next decade, there is a need to develop the existing AO technologies for large aperture telescopes. Some aspects of this development are highlighted. Finally, the recent AO developments in India are also presented.

  19. Wavefront reconstruction in adaptive optics systems using nonlinear multivariate splines.

    Science.gov (United States)

    de Visser, Cornelis C; Verhaegen, Michel

    2013-01-01

    This paper presents a new method for zonal wavefront reconstruction (WFR) with application to adaptive optics systems. This new method, indicated as Spline based ABerration REconstruction (SABRE), uses bivariate simplex B-spline basis functions to reconstruct the wavefront using local wavefront slope measurements. The SABRE enables WFR on nonrectangular and partly obscured sensor grids and is not subject to the waffle mode. The performance of SABRE is compared to that of the finite difference (FD) method in numerical experiments using data from a simulated Shack-Hartmann lenslet array. The results show that SABRE offers superior reconstruction accuracy and noise rejection capabilities compared to the FD method.

  20. Optical diffraction pattern measurements using a self-scanning photodiode array interfaced to a microcomputer

    Science.gov (United States)

    Wesley, James T.; Behof, Anthony F.

    1987-09-01

    A system for recording and analyzing optical diffraction patterns using a self-scanning photodiode array interfaced to a popular microcomputer is described. The detector array consists of 128 photodiodes on 25-μm centers and is sensitive to light in the visible portion of the spectrum. Details of the interface between the array and an Apple II microcomputer are given. The overall performance of the system is demonstrated for Fresnel diffraction by a single slit.

  1. Harnessing Adaptive Optics for Space Debris Collision Mitigation

    Science.gov (United States)

    Zovaro, A.; Bennet, F.; Copeland, M.; Rigaut, F.; d'Orgeville, C.; Grosse, D.

    2016-09-01

    Human kind's continued use of space depends upon minimising the build-up of debris in low Earth-orbit (LEO). Preventing collisions between satellites and debris is essential given that a single collision can generate thousands of new debris objects. However, in-orbit manoeuvring of satellites is extremely expensive and shortens their operational life. Adjusting the orbits of debris objects instead of satellites would shift the responsibility of collision avoidance away from satellite operators altogether, thereby offering a superior solution. The Research School of Astronomy and Astrophysics at the Australian National University, partnered with Electro Optic Systems (EOS) Space Systems, Lockheed Martin Corporation and the Space Environment Research Centre (SERC) Limited, are developing the Adaptive Optics Tracking and Pushing (AOTP) system. AOTP will be used to perturb the orbits of debris objects using photon pressure from a 10 kW IR laser beam launched from the 1.8 m telescope at Mount. Stromlo Observatory, Australia. Initial simulations predict that AOTP will be able to displace debris objects 10 cm in size by up to 100 m with several overhead passes. An operational demonstrator is planned for 2019. Turbulence will distort the laser beam as it propagates through the atmosphere, resulting in a lower photon flux on the target and reduced pointing accuracy. To mitigate these effects, adaptive optics (AO) will be used to apply wavefront correction to the beam prior to launch. A unique challenge in designing the AO system arises from the high slew rate needed to track objects in LEO, which in turn requires laser guide star AO for satisfactory wavefront correction. The optical design and results from simulations of estimated performance of AOTP will be presented. In particular, design considerations associated with the high-power laser will be detailed.

  2. In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography.

    Science.gov (United States)

    Wong, Kevin S K; Jian, Yifan; Cua, Michelle; Bonora, Stefano; Zawadzki, Robert J; Sarunic, Marinko V

    2015-02-01

    Wavefront sensorless adaptive optics optical coherence tomography (WSAO-OCT) is a novel imaging technique for in vivo high-resolution depth-resolved imaging that mitigates some of the challenges encountered with the use of sensor-based adaptive optics designs. This technique replaces the Hartmann Shack wavefront sensor used to measure aberrations with a depth-resolved image-driven optimization algorithm, with the metric based on the OCT volumes acquired in real-time. The custom-built ultrahigh-speed GPU processing platform and fast modal optimization algorithm presented in this paper was essential in enabling real-time, in vivo imaging of human retinas with wavefront sensorless AO correction. WSAO-OCT is especially advantageous for developing a clinical high-resolution retinal imaging system as it enables the use of a compact, low-cost and robust lens-based adaptive optics design. In this report, we describe our WSAO-OCT system for imaging the human photoreceptor mosaic in vivo. We validated our system performance by imaging the retina at several eccentricities, and demonstrated the improvement in photoreceptor visibility with WSAO compensation.

  3. Design of the Dual Conjugate Adaptive Optics Test-bed

    Science.gov (United States)

    Sharf, Inna; Bell, K.; Crampton, D.; Fitzsimmons, J.; Herriot, Glen; Jolissaint, Laurent; Lee, B.; Richardson, H.; van der Kamp, D.; Veran, Jean-Pierre

    In this paper, we describe the Multi-Conjugate Adaptive Optics laboratory test-bed presently under construction at the University of Victoria, Canada. The test-bench will be used to support research in the performance of multi-conjugate adaptive optics, turbulence simulators, laser guide stars and miniaturizing adaptive optics. The main components of the test-bed include two micro-machined deformable mirrors, a tip-tilt mirror, four wavefront sensors, a source simulator, a dual-layer turbulence simulator, as well as computational and control hardware. The paper will describe in detail the opto-mechanical design of the adaptive optics module, the design of the hot-air turbulence generator and the configuration chosen for the source simulator. Below, we present a summary of these aspects of the bench. The optical and mechanical design of the test-bed has been largely driven by the particular choice of the deformable mirrors. These are continuous micro-machined mirrors manufactured by Boston Micromachines Corporation. They have a clear aperture of 3.3 mm and are deformed with 140 actuators arranged in a square grid. Although the mirrors have an open-loop bandwidth of 6.6 KHz, their shape can be updated at a sampling rate of 100 Hz. In our optical design, the mirrors are conjugated at 0km and 10 km in the atmosphere. A planar optical layout was achieved by using four off-axis paraboloids and several folding mirrors. These optics will be mounted on two solid blocks which can be aligned with respect to each other. The wavefront path design accommodates 3 monochromatic guide stars that can be placed at either 90 km or at infinity. The design relies on the natural separation of the beam into 3 parts because of differences in locations of the guide stars in the field of view. In total four wavefront sensors will be procured from Adaptive Optics Associates (AOA) or built in-house: three for the guide stars and the fourth to collect data from the science source output in

  4. Adaptive spectral window sizes for feature extraction from optical spectra

    Science.gov (United States)

    Kan, Chih-Wen; Lee, Andy Y.; Pham, Nhi; Nieman, Linda T.; Sokolov, Konstantin; Markey, Mia K.

    2008-02-01

    We propose an approach to adaptively adjust the spectral window size used to extract features from optical spectra. Previous studies have employed spectral features extracted by dividing the spectra into several spectral windows of a fixed width. However, the choice of spectral window size was arbitrary. We hypothesize that by adaptively adjusting the spectral window sizes, the trends in the data will be captured more accurately. Our method was tested on a diffuse reflectance spectroscopy dataset obtained in a study of oblique polarization reflectance spectroscopy of oral mucosa lesions. The diagnostic task is to classify lesions into one of four histopathology groups: normal, benign, mild dysplasia, or severe dysplasia (including carcinoma). Nine features were extracted from each of the spectral windows. We computed the area (AUC) under Receiver Operating Characteristic curve to select the most discriminatory wavelength intervals. We performed pairwise classifications using Linear Discriminant Analysis (LDA) with leave-one-out cross validation. The results showed that for discriminating benign lesions from mild or severe dysplasia, the adaptive spectral window size features achieved AUC of 0.84, while a fixed spectral window size of 20 nm had AUC of 0.71, and an AUC of 0.64 is achieved with a large window size containing all wavelengths. The AUCs of all feature combinations were also calculated. These results suggest that the new adaptive spectral window size method effectively extracts features that enable accurate classification of oral mucosa lesions.

  5. SU-F-T-235: Optical Scan Based Collision Avoidance Using Multiple Stereotactic Cameras During Simulation

    Energy Technology Data Exchange (ETDEWEB)

    Cardan, R; Popple, R; Dobelbower, M; De Los Santos, J; Fiveash, J [The University of Alabama at Birmingham, Birmingham, AL (United States)

    2016-06-15

    Purpose: To demonstrate the ability to quickly generate an accurate collision avoidance map using multiple stereotactic cameras during simulation. Methods: Three Kinect stereotactic cameras were placed in the CT simulation room and optically calibrated to the DICOM isocenter. Immediately before scanning, the patient was optically imaged to generate a 3D polygon mesh, which was used to calculate the collision avoidance area using our previously developed framework. The mesh was visually compared to the CT scan body contour to ensure accurate coordinate alignment. To test the accuracy of the collision calculation, the patient and machine were physically maneuvered in the treatment room to calculated collision boundaries. Results: The optical scan and collision calculation took 38.0 seconds and 2.5 seconds to complete respectively. The collision prediction accuracy was determined using a receiver operating curve (ROC) analysis, where the true positive, true negative, false positive and false negative values were 837, 821, 43, and 79 points respectively. The ROC accuracy was 93.1% over the sampled collision space. Conclusion: We have demonstrated a framework which is fast and accurate for predicting collision avoidance for treatment which can be determined during the normal simulation process. Because of the speed, the system could be used to add a layer of safety with a negligible impact on the normal patient simulation experience. This information could be used during treatment planning to explore the feasible geometries when optimizing plans. Research supported by Varian Medical Systems.

  6. Potential Challenges in Near-Field Scanning Optical Microscopy for Space Applications

    Science.gov (United States)

    Vikram, Chandra S.; Witherow, William K.; Rose, M. Franklin (Technical Monitor)

    2000-01-01

    Near-field scanning optical microscopy (NSOM) also called scanning near-field optical microscopy (SNOM) is now well accepted as a powerful tool for sub-wavelength (nanoscale in the optical region) spatial resolution microscopy and a large number of related tasks. The importance lies in the fact of strategic advantages of standard microscopy but with significantly enhanced resolution. Since many modern optical diagnostic techniques have found useful applications in space, it is logical to consider the future role of NSOM in such situations. For example, protein crystal growth study under microgravity conditions is a valid candidate. If applied successfully, processes at molecular level can be studied during the growth. NSOM has already been demonstrated to be useful for the study of such crystals here on earth. The basic principle of NSOM can be illustrated. The illumination-collection mode is shown although several other possible approaches exist. In this, the sample is illuminated and the light from the sample is collected through the same tiny aperture opening. A tapered optical fiber is scanned near the sample surface. The tip is coated generally with a metal with a sub-wavelength aperture opening. The tip-sample distance is maintained constant while scanning. Thus, the optical signal available for collection is generally a function of the optical properties of the sample surface. Since the aperture is sub-wavelength in diameter and the tip is held very close (again in the sub-wavelength domain) to the surface, the lateral resolution in the sub-wavelength domain is obtained. Thus, the typical wavelength- order resolution of ordinary microscopy can be significantly enhanced while maintaining the strategic advantages (no need of sample in vacuum chamber, electron beams, etc). Commercial NSOM systems play a key role in the success and widespread acceptance of the tool. These commercial systems work fairly well in laboratory conditions on earth. However, they may

  7. A correlative optical microscopy and scanning electron microscopy approach to locating nanoparticles in brain tumors.

    Science.gov (United States)

    Kempen, Paul J; Kircher, Moritz F; de la Zerda, Adam; Zavaleta, Cristina L; Jokerst, Jesse V; Mellinghoff, Ingo K; Gambhir, Sanjiv S; Sinclair, Robert

    2015-01-01

    The growing use of nanoparticles in biomedical applications, including cancer diagnosis and treatment, demands the capability to exactly locate them within complex biological systems. In this work a correlative optical and scanning electron microscopy technique was developed to locate and observe multi-modal gold core nanoparticle accumulation in brain tumor models. Entire brain sections from mice containing orthotopic brain tumors injected intravenously with nanoparticles were imaged using both optical microscopy to identify the brain tumor, and scanning electron microscopy to identify the individual nanoparticles. Gold-based nanoparticles were readily identified in the scanning electron microscope using backscattered electron imaging as bright spots against a darker background. This information was then correlated to determine the exact location of the nanoparticles within the brain tissue. The nanoparticles were located only in areas that contained tumor cells, and not in the surrounding healthy brain tissue. This correlative technique provides a powerful method to relate the macro- and micro-scale features visible in light microscopy with the nanoscale features resolvable in scanning electron microscopy. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Extended depth of focus adaptive optics spectral domain optical coherence tomography

    Science.gov (United States)

    Sasaki, Kazuhiro; Kurokawa, Kazuhiro; Makita, Shuichi; Yasuno, Yoshiaki

    2012-01-01

    We present an adaptive optics spectral domain optical coherence tomography (AO-SDOCT) with a long focal range by active phase modulation of the pupil. A long focal range is achieved by introducing AO-controlled third-order spherical aberration (SA). The property of SA and its effects on focal range are investigated in detail using the Huygens-Fresnel principle, beam profile measurement and OCT imaging of a phantom. The results indicate that the focal range is extended by applying SA, and the direction of extension can be controlled by the sign of applied SA. Finally, we demonstrated in vivo human retinal imaging by altering the applied SA. PMID:23082278

  9. Optical CT scanning of cross-linked radiochromic gel without cylinder wall

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, Kevin [London Regional Cancer Program, London Health Sciences Centre and Department of Medical Biophysics, University of Western Ontario, London, ON (Canada)], E-mail: kevin.jordan@lhsc.on.ca

    2009-05-01

    Genipin cross-linked gelatin hydrogels with 0.2 M sulphuric acid are radiochromic and have sufficient sensitivity for investigating doses less than 50 Gray. Because of the gelatine cross-links, these gels have sufficient strength to allow removal from the vessel container in which they were cast. Placing the gels in the same liquid that was used for preparing the gel allows the radiochemistry to the same throughout the gels and provides physical support. In this buoyancy neutral environment the gel has the same shape and the preparation vessel. This allows optical CT scanning without wall artefacts due to reflection, refraction and optical activity. A gel was irradiated to dose of 25 Gray with a 10 MV photon beam of 20 x 20 mm cross section. Full 3D optical CT scanning was performed with a Vista 10 optical cone-beam CT scanner. Central beam axis profiles and depth dose agree with diode and parallel plate ion chamber measurements. These results demonstrate that genipin cross-linked gel can be used for accurate 3D dosimetry, including surface dose measurements.

  10. Comparison between optical-resolution photoacoustic microscopy and confocal laser scanning microscopy for turbid sample imaging.

    Science.gov (United States)

    U-Thainual, Paweena; Kim, Do-Hyun

    2015-12-01

    Optical-resolution photoacoustic microscopy (ORPAM) in theory provides lateral resolution equivalent to the optical diffraction limit. Scattering media, such as biological turbid media, attenuates the optical signal and also alters the diffraction-limited spot size of the focused beam. The ORPAM signal is generated only from a small voxel in scattering media with dimensions equivalent to the laser spot size after passing through scattering layers and is detected by an acoustic transducer, which is not affected by optical scattering. Thus, both ORPAM and confocal laser scanning microscopy (CLSM) reject scattered light. A multimodal optical microscopy platform that includes ORPAM and CLSM was constructed, and the lateral resolution of both modes was measured using patterned thin metal film with and without a scattering barrier. The effect of scattering media on the lateral resolution was studied using different scattering coefficients and was compared to computational results based on Monte Carlo simulations. It was found that degradation of lateral resolution due to optical scattering was not significant for either ORPAM or CLSM. The depth discrimination capability of ORPAM and CLSM was measured using microfiber embedded in a light scattering phantom material. ORPAM images demonstrated higher contrast compared to CLSM images partly due to reduced acoustic signal scattering.

  11. Spectroscopic infrared scanning near-field optical microscopy (IR-SNOM)

    Energy Technology Data Exchange (ETDEWEB)

    Vobornik, D. [Institut de Physique de la Matiere Complexe, Ecole Polytechnique Federale de Lausanne (EPFL), Station 3, CH-1015 Lausanne (Switzerland)]. E-mail: dusan.vobornik@epfl.ch; Margaritondo, G. [Institut de Physique de la Matiere Complexe, Ecole Polytechnique Federale de Lausanne (EPFL), Station 3, CH-1015 Lausanne (Switzerland); Sanghera, J.S. [Optical Sciences Division, U.S. Naval Research Laboratory, 4555 Overlook Avenue SE, Washington, DC 20375 (United States); Thielen, P. [Optical Sciences Division, U.S. Naval Research Laboratory, 4555 Overlook Avenue SE, Washington, DC 20375 (United States); Aggarwal, I.D. [Optical Sciences Division, U.S. Naval Research Laboratory, 4555 Overlook Avenue SE, Washington, DC 20375 (United States); Ivanov, B. [Department of Physics and Astronomy, Vanderbilt University, Nashville, TN 31235 (United States); Tolk, N.H. [Department of Physics and Astronomy, Vanderbilt University, Nashville, TN 31235 (United States); Manni, V. [Institute of Neurobiology and Molecular Medicine, 00133 Rome (Italy); Grimaldi, S. [Institute of Neurobiology and Molecular Medicine, 00133 Rome (Italy); Lisi, A. [Institute of Neurobiology and Molecular Medicine, 00133 Rome (Italy); Rieti, S. [Institute of Neurobiology and Molecular Medicine, 00133 Rome (Italy); Piston, D.W. [Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232 (United States); Generosi, R. [Istituto di Stuttura della Materia, via Fosso del Cavaliere 100, 00133 Rome (Italy); Luce, M. [Istituto di Stuttura della Materia, via Fosso del Cavaliere 100, 00133 Rome (Italy); Perfetti, P. [Istituto di Stuttura della Materia, via Fosso del Cavaliere 100, 00133 Rome (Italy); Cricenti, A. [Istituto di Stuttura della Materia, via Fosso del Cavaliere 100, 00133 Rome (Italy)

    2005-09-29

    Scanning near-field optical microscopy (SNOM or NSOM) is the technique with the highest lateral optical resolution available today, while infrared (IR) spectroscopy has a high chemical specificity. Combining SNOM with a tunable IR source produces a unique tool, IR-SNOM, capable of imaging distributions of chemical species with a 100 nm spatial resolution. We present in this paper boron nitride (BN) thin film images, where IR-SNOM shows the distribution of hexagonal and cubic phases within the sample. Exciting potential applications in biophysics and medical sciences are illustrated with SNOM images of the distribution of different chemical species within cells. We present in this article images with resolutions of the order of {lambda}/60 with SNOM working with infrared light. With our SNOM setup, we routinely get optical resolutions between 50 and 150 nm, regardless of the wavelength of the light used to illuminate the sample.

  12. Phenotypic diversity in autosomal-dominant cone-rod dystrophy elucidated by adaptive optics retinal imaging.

    Science.gov (United States)

    Song, Hongxin; Rossi, Ethan A; Stone, Edwin; Latchney, Lisa; Williams, David; Dubra, Alfredo; Chung, Mina

    2018-01-01

    Several genes causing autosomal-dominant cone-rod dystrophy (AD-CRD) have been identified. However, the mechanisms by which genetic mutations lead to cellular loss in human disease remain poorly understood. Here we combine genotyping with high-resolution adaptive optics retinal imaging to elucidate the retinal phenotype at a cellular level in patients with AD-CRD harbouring a defect in the GUCA1A gene. Nine affected members of a four-generation AD-CRD pedigree and three unaffected first-degree relatives underwent clinical examinations including visual acuity, fundus examination, Goldmann perimetry, spectral domain optical coherence tomography and electroretinography. Genome-wide scan followed by bidirectional sequencing was performed on all affected participants. High-resolution imaging using a custom adaptive optics scanning light ophthalmoscope (AOSLO) was performed for selected participants. Clinical evaluations showed a range of disease severity from normal fundus appearance in teenaged patients to pronounced macular atrophy in older patients. Molecular genetic testing showed a mutation in in GUCA1A segregating with disease. AOSLO imaging revealed that of the two teenage patients with mild disease, one had severe disruption of the photoreceptor mosaic while the other had a normal cone mosaic. AOSLO imaging demonstrated variability in the pattern of cone and rod cell loss between two teenage cousins with early AD-CRD, who had similar clinical features and had the identical disease-causing mutation in GUCA1A . This finding suggests that a mutation in GUCA1A does not lead to the same degree of AD-CRD in all patients. Modifying factors may mitigate or augment disease severity, leading to different retinal cellular phenotypes. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  13. High-resolution adaptive optics retinal imaging of cellular structure in choroideremia.

    Science.gov (United States)

    Morgan, Jessica I W; Han, Grace; Klinman, Eva; Maguire, William M; Chung, Daniel C; Maguire, Albert M; Bennett, Jean

    2014-09-04

    We characterized retinal structure in patients and carriers of choroideremia using adaptive optics and other high resolution modalities. A total of 57 patients and 18 carriers of choroideremia were imaged using adaptive optics scanning light ophthalmoscopy (AOSLO), optical coherence tomography (OCT), autofluorescence (AF), and scanning light ophthalmoscopy (SLO). Cone density was measured in 59 eyes of 34 patients where the full cone mosaic was observed. The SLO imaging revealed scalloped edges of RPE atrophy and large choroidal vessels. The AF imaging showed hypo-AF in areas of degeneration, while central AF remained present. OCT images showed outer retinal tubulations and thinned RPE/interdigitation layers. The AOSLO imaging revealed the cone mosaic in central relatively intact retina, and cone density was either reduced or normal at 0.5 mm eccentricity. The border of RPE atrophy showed abrupt loss of the cone mosaic at the same location. The AF imaging in comparison with AOSLO showed RPE health may be compromised before cone degeneration. Other disease features, including visualization of choroidal vessels, hyper-reflective clumps of cones, and unique retinal findings, were tabulated to show the frequency of occurrence and model disease progression. The data support the RPE being one primary site of degeneration in patients with choroideremia. Photoreceptors also may degenerate independently. High resolution imaging, particularly AOSLO in combination with OCT, allows single cell analysis of disease in choroideremia. These modalities promise to be useful in monitoring disease progression, and in documenting the efficacy of gene and cell-based therapies for choroideremia and other diseases as these therapies emerge. (ClinicalTrials.gov number, NCT01866371.). Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.

  14. Photometric Calibration of the Gemini South Adaptive Optics Imager

    Science.gov (United States)

    Stevenson, Sarah Anne; Rodrigo Carrasco Damele, Eleazar; Thomas-Osip, Joanna

    2017-01-01

    The Gemini South Adaptive Optics Imager (GSAOI) is an instrument available on the Gemini South telescope at Cerro Pachon, Chile, utilizing the Gemini Multi-Conjugate Adaptive Optics System (GeMS). In order to allow users to easily perform photometry with this instrument and to monitor any changes in the instrument in the future, we seek to set up a process for performing photometric calibration with standard star observations taken across the time of the instrument’s operation. We construct a Python-based pipeline that includes IRAF wrappers for reduction and combines the AstroPy photutils package and original Python scripts with the IRAF apphot and photcal packages to carry out photometry and linear regression fitting. Using the pipeline, we examine standard star observations made with GSAOI on 68 nights between 2013 and 2015 in order to determine the nightly photometric zero points in the J, H, Kshort, and K bands. This work is based on observations obtained at the Gemini Observatory, processed using the Gemini IRAF and gemini_python packages, which is operated by the Association of Universities for Research in Astronomy, Inc., under a cooperative agreement with the NSF on behalf of the Gemini partnership: the National Science Foundation (United States), the National Research Council (Canada), CONICYT (Chile), Ministerio de Ciencia, Tecnología e Innovación Productiva (Argentina), and Ministério da Ciência, Tecnologia e Inovação (Brazil).

  15. A New, Adaptable, Optical High-Resolution 3-Axis Sensor

    Directory of Open Access Journals (Sweden)

    Niels Buchhold

    2017-01-01

    Full Text Available This article presents a new optical, multi-functional, high-resolution 3-axis sensor which serves to navigate and can, for example, replace standard joysticks in medical devices such as electric wheelchairs, surgical robots or medical diagnosis devices. A light source, e.g., a laser diode, is affixed to a movable axis and projects a random geometric shape on an image sensor (CMOS or CCD. The downstream microcontroller’s software identifies the geometric shape’s center, distortion and size, and then calculates x, y, and z coordinates, which can be processed in attached devices. Depending on the image sensor in use (e.g., 6.41 megapixels, the 3-axis sensor features a resolution of 1544 digits from right to left and 1038 digits up and down. Through interpolation, these values rise by a factor of 100. A unique feature is the exact reproducibility (deflection to coordinates and its precise ability to return to its neutral position. Moreover, optical signal processing provides a high level of protection against electromagnetic and radio frequency interference. The sensor is adaptive and adjustable to fit a user’s range of motion (stroke and force. This recommendation aims to optimize sensor systems such as joysticks in medical devices in terms of safety, ease of use, and adaptability.

  16. Adaptive Optics Imaging Survey of Luminous Infrared Galaxies

    Energy Technology Data Exchange (ETDEWEB)

    Laag, E A; Canalizo, G; van Breugel, W; Gates, E L; de Vries, W; Stanford, S A

    2006-03-13

    We present high resolution imaging observations of a sample of previously unidentified far-infrared galaxies at z < 0.3. The objects were selected by cross-correlating the IRAS Faint Source Catalog with the VLA FIRST catalog and the HST Guide Star Catalog to allow for adaptive optics observations. We found two new ULIGs (with L{sub FIR} {ge} 10{sup 12} L{sub {circle_dot}}) and 19 new LIGs (with L{sub FIR} {ge} 10{sup 11} L{sub {circle_dot}}). Twenty of the galaxies in the sample were imaged with either the Lick or Keck adaptive optics systems in H or K{prime}. Galaxy morphologies were determined using the two dimensional fitting program GALFIT and the residuals examined to look for interesting structure. The morphologies reveal that at least 30% are involved in tidal interactions, with 20% being clear mergers. An additional 50% show signs of possible interaction. Line ratios were used to determine powering mechanism; of the 17 objects in the sample showing clear emission lines--four are active galactic nuclei and seven are starburst galaxies. The rest exhibit a combination of both phenomena.

  17. AVES: an adaptive optics visual echelle spectrograph for the VLT

    Science.gov (United States)

    Pasquini, Luca; Delabre, Bernard; Avila, Gerardo; Bonaccini, Domenico

    1998-07-01

    We present the preliminary study of a low cost, high performance spectrograph for the VLT, for observations in the V, R and I bands. This spectrograph is meant for intermediate (R equals 16,000) resolution spectroscopy of faint (sky and/or detector limited) sources, with particular emphasis on the study of solar-type (F-G) stars belonging to the nearest galaxies and to distant (or highly reddened) galactic clusters. The spectrograph is designed to use the adaptive optics (AO) systems at the VLT Telescope. Even if these AO systems will not provide diffraction limited images in the V, R and I bands, the photon concentration will still be above approximately 60% of the flux in an 0.3 arcsecond aperture for typical Paranal conditions. This makes the construction of a compact, cheap and efficient echelle spectrograph possible. AVES will outperform comparable non adaptive optic instruments by more than one magnitude for sky- and/or detector-limited observations, and it will be very suitable for observations in crowded fields.

  18. CHARACTERIZATION AND CORRELATION OF "JAMPOL DOTS" ON ADAPTIVE OPTICS WITH FOVEAL GRANULARITY ON CONVENTIONAL FUNDUS IMAGING.

    Science.gov (United States)

    Onishi, Alex C; Roberts, Philipp K; Jampol, Lee M; Nesper, Peter L; Fawzi, Amani A

    2017-11-22

    To describe features characteristic of multiple evanescent white dot syndrome (MEWDS) using adaptive optics scanning laser ophthalmoscopy (AOSLO). Six women (seven eyes) who presented with MEWDS between June 2014 and April 2017 underwent ophthalmologic examinations and multimodal imaging including infrared, AOSLO, and spectral domain optical coherence tomography. Bright hyperreflective lesions on AOSLO throughout the course of MEWDS could be correlated to the hyperreflective dots of foveal granularity on infrared imaging without apparent corresponding changes on spectral domain optical coherence tomography. During the acute phase of MEWDS, extrafoveal hyperreflective dots were also visible on AOSLO and infrared and were associated with accumulations of hyperreflective material above the retinal pigment epithelium on spectral domain optical coherence tomography. Foveal granularity on conventional fundus imaging could be correlated with hyperreflective lesions visible on AOSLO. We hypothesize that these hyperreflective lesions, "Jampol dots," are the foveal corollaries of the same process associated with the classic "dot" lesions in MEWDS. Based on the intact photoreceptor mosaic on AOSLO, we surmise that this material is accumulating at the level of the retinal pigment epithelium.

  19. Control algorithms and applications of the wavefront sensorless adaptive optics

    Science.gov (United States)

    Ma, Liang; Wang, Bin; Zhou, Yuanshen; Yang, Huizhen

    2017-10-01

    Compared with the conventional adaptive optics (AO) system, the wavefront sensorless (WFSless) AO system need not to measure the wavefront and reconstruct it. It is simpler than the conventional AO in system architecture and can be applied to the complex conditions. Based on the analysis of principle and system model of the WFSless AO system, wavefront correction methods of the WFSless AO system were divided into two categories: model-free-based and model-based control algorithms. The WFSless AO system based on model-free-based control algorithms commonly considers the performance metric as a function of the control parameters and then uses certain control algorithm to improve the performance metric. The model-based control algorithms include modal control algorithms, nonlinear control algorithms and control algorithms based on geometrical optics. Based on the brief description of above typical control algorithms, hybrid methods combining the model-free-based control algorithm with the model-based control algorithm were generalized. Additionally, characteristics of various control algorithms were compared and analyzed. We also discussed the extensive applications of WFSless AO system in free space optical communication (FSO), retinal imaging in the human eye, confocal microscope, coherent beam combination (CBC) techniques and extended objects.

  20. Development of adaptive optics elements for solar telescope

    Science.gov (United States)

    Lukin, V. P.; Grigor'ev, V. M.; Antoshkin, L. V.; Botugina, N. N.; Kovadlo, P. G.; Konyaev, P. A.; Kopulov, E. A.; Skomorovsky, V. I.; Trifonov, V. D.; Chuprakov, S. A.

    2012-07-01

    The devices and components of adaptive optical system ANGARA, which is developed for image correction in the Big solar vacuum telescope (BSVT) at Baykal astrophysical observatory are described. It is shown that the use of modernized adaptive system on BSVT not only reduces the turbulent atmospheric distortions of image, but also gives a possibility to improve the telescope developing new methods of solar observations. A high precision Shack-Hartmann wavefront (WF) sensor has been developed on the basis of a low-aperture off-axis diffraction lens array. The device is capable of measuring WF slopes at array sub-apertures of size 640X640 μm with an error not exceeding 4.80 arc.sec. Also the modification of this sensor for adaptive system of solar telescope using extended scenes as tracking objects, such as sunspot, pores, solar granulation and limb, is presented. The software package developed for the proposed WF sensors includes three algorithms of local WF slopes estimation (modified centroids, normalized cross-correlation and fast Fourier-demodulation), as well as three methods of WF reconstruction (modal Zernike polynomials expansion, deformable mirror response functions expansion and phase unwrapping), that can be selected during operation with accordance to the application.

  1. Statistical learning methods for aero-optic wavefront prediction and adaptive-optic latency compensation

    Science.gov (United States)

    Burns, W. Robert

    Since the early 1970's research in airborne laser systems has been the subject of continued interest. Airborne laser applications depend on being able to propagate a near diffraction-limited laser beam from an airborne platform. Turbulent air flowing over the aircraft produces density fluctuations through which the beam must propagate. Because the index of refraction of the air is directly related to the density, the turbulent flow imposes aberrations on the beam passing through it. This problem is referred to as Aero-Optics. Aero-Optics is recognized as a major technical issue that needs to be solved before airborne optical systems can become routinely fielded. This dissertation research specifically addresses an approach to mitigating the deleterious effects imposed on an airborne optical system by aero-optics. A promising technology is adaptive optics: a feedback control method that measures optical aberrations and imprints the conjugate aberrations onto an outgoing beam. The challenge is that it is a computationally-difficult problem, since aero-optic disturbances are on the order of kilohertz for practical applications. High control loop frequencies and high disturbance frequencies mean that adaptive-optic systems are sensitive to latency in sensors, mirrors, amplifiers, and computation. These latencies build up to result in a dramatic reduction in the system's effective bandwidth. This work presents two variations of an algorithm that uses model reduction and data-driven predictors to estimate the evolution of measured wavefronts over a short temporal horizon and thus compensate for feedback latency. The efficacy of the two methods are compared in this research, and evaluated against similar algorithms that have been previously developed. The best version achieved over 75% disturbance rejection in simulation in the most optically active flow region in the wake of a turret, considerably outperforming conventional approaches. The algorithm is shown to be

  2. Binary stars observed with adaptive optics at the starfire optical range

    Energy Technology Data Exchange (ETDEWEB)

    Drummond, Jack D. [Air Force Research Laboratory, Directed Energy Directorate, RDSAM, 3550 Aberdeen Avenue SE, Kirtland AFB, NM 87117-5776 (United States)

    2014-03-01

    In reviewing observations taken of binary stars used as calibration objects for non-astronomical purposes with adaptive optics on the 3.5 m Starfire Optical Range telescope over the past 2 years, one-fifth of them were found to be off-orbit. In order to understand such a high number of discrepant position angles and separations, all previous observations in the Washington Double Star Catalog for these rogue binaries were obtained from the Naval Observatory. Adding our observations to these yields new orbits for all, resolving the discrepancies. We have detected both components of γ Gem for the first time, and we have shown that 7 Cam is an optical pair, not physically bound.

  3. Compact MEMS-based Adaptive Optics Optical Coherence Tomography for Clinical Use

    Energy Technology Data Exchange (ETDEWEB)

    Chen, D; Olivier, S; Jones, S; Zawadzki, R; Evans, J; Choi, S; Werner, J

    2008-02-04

    We describe a compact MEMS-based adaptive optics (AO) optical coherence tomography system with improved AO performance and ease of clinical use. A typical AO system consists of a Shack-Hartmann wavefront sensor and a deformable mirror that measures and corrects the ocular and system aberrations. Because of the limitation on the current deformable mirror technologies, the amount of real-time ocular-aberration compensation is restricted and small in the previous AO-OCT instruments. In this instrument, we proposed to add an optical apparatus to correct the spectacle aberrations of the patients such as myopia, hyperopia and astigmatism. This eliminated the tedious process of the trial lenses in clinical imaging. Different amount of spectacle aberration compensation was achieved by motorized stages and automated with the AO computer for ease of clinical use. In addition, the compact AO-OCT was optimized to have minimum system aberrations to reduce AO registration errors and improve AO performance.

  4. A Review of Adaptive Optics Optical Coherence Tomography: Technical Advances, Scientific Applications, and the Future.

    Science.gov (United States)

    Jonnal, Ravi S; Kocaoglu, Omer P; Zawadzki, Robert J; Liu, Zhuolin; Miller, Donald T; Werner, John S

    2016-07-01

    Optical coherence tomography (OCT) has enabled "virtual biopsy" of the living human retina, revolutionizing both basic retina research and clinical practice over the past 25 years. For most of those years, in parallel, adaptive optics (AO) has been used to improve the transverse resolution of ophthalmoscopes to foster in vivo study of the retina at the microscopic level. Here, we review work done over the last 15 years to combine the microscopic transverse resolution of AO with the microscopic axial resolution of OCT, building AO-OCT systems with the highest three-dimensional resolution of any existing retinal imaging modality. We surveyed the literature to identify the most influential antecedent work, important milestones in the development of AO-OCT technology, its applications that have yielded new knowledge, research areas into which it may productively expand, and nascent applications that have the potential to grow. Initial efforts focused on demonstrating three-dimensional resolution. Since then, many improvements have been made in resolution and speed, as well as other enhancements of acquisition and postprocessing techniques. Progress on these fronts has produced numerous discoveries about the anatomy, function, and optical properties of the retina. Adaptive optics OCT continues to evolve technically and to contribute to our basic and clinical knowledge of the retina. Due to its capacity to reveal cellular and microscopic detail invisible to clinical OCT systems, it is an ideal companion to those instruments and has the demonstrable potential to produce images that can guide the interpretation of clinical findings.

  5. Scanning laser polarimetry quantification of retinal nerve fiber layer thinning following optic neuritis.

    Science.gov (United States)

    Trip, S Anand; Schlottmann, Patricio G; Jones, Stephen J; Kallis, Constantinos; Altmann, Daniel R; Garway-Heath, David F; Thompson, Alan J; Plant, Gordon T; Miller, David H

    2010-09-01

    Several studies with optical coherence tomography (OCT) have demonstrated thinning of the retinal nerve fiber layer (RNFL) in patients with optic neuritis and multiple sclerosis. Similar studies have not been performed with scanning laser polarimetry (SLP), which relies on different physical phenomena. This study was designed to use SLP to measure axonal loss following a single episode of optic neuritis and to determine if there is a relationship between the degree of axonal loss and the degree of residual visual dysfunction. Twenty-five patients with a single episode of optic neuritis and 15 control subjects were studied with SLP using the GDxVCC device to determine RNFL thickness in relation to visual acuity, visual fields, color vision, visual evoked potentials (VEPs), and previously published OCT data. SLP detected significant RNFL thinning in affected eyes compared to clinically unaffected fellow eyes in patients and in control eyes (P color vision. RNFL thinning correlated with reduced whole visual field and central visual field measures and VEP amplitudes. Superior and inferior quadrant RNFL thinning was related to corresponding regional visual field loss. There was a scaling factor between SLP and OCT RNFL measurements but only modest agreement. SLP detected functionally relevant axonal loss in eyes affected by optic neuritis. There was a scaling factor between RNFL measurements obtained with SLP and OCT but only modest agreement. Care should therefore be taken when comparing RNFL data from studies using these different devices.

  6. Aspheric optical surface profiling based on laser scanning and auto-collimation

    Science.gov (United States)

    Xie, Hongbo; Jiang, Min; Wang, Yao; Pang, Xiaotian; Wang, Chao; Su, Yongpeng; Yang, Lei

    2017-11-01

    Nowadays the utilization of aspheric lenses has become more and more popular, enabling highly increased degree of freedom for optical design and simultaneously improving the performance of optical systems. Fast and accurate surface profiling of these aspheric components is a real demand in characterization and optimization of the optical systems. In this paper, a novel and simple surface profiler instrument is designed and developed to fulfill the ever increasing need of testing the axially symmetric aspheric surface. The proposed instrument is implemented based on a unique mapping between the position and rotation angle of the reflective mirror in optical path and the coordinate of reflection point on the surface during rapid laser beam scanning. High accuracy of the proposed surface profiling method is ensured by a high-resolution grating guide rail, indexing plate, and position sensitive detector based on laser auto-collimation and beam center-fitting. Testing the meridian line of both convex and concave surfaces has been experimentally demonstrated using the developed instrument. In comparison to tested results from conventional image measuring instruments and coordinate measuring machines, coefficient of determination better than 0.999 99 and RMS less than 1.5 μm have been achieved, which validates the feasibility of this method. Analysis on the systematic error is beneficial to further improve its measurement accuracy. The presented instrument—essentially builds on the geometrical optics technique—provides a powerful tool to measure the aspheric surfaces quickly and accurately with stable structure and simple algorithm.

  7. Nonlinear optical characterization of graphite oxide thin film by open aperture Z-scan technique

    Energy Technology Data Exchange (ETDEWEB)

    Sreeja, V. G.; Reshmi, R.; Devasia, Sebin; Anila, E. I., E-mail: anilaei@gmail.com [Optolectronic and Nanomaterials Research Laboratory, Department of Physics, Union Christian College, Aluva-683 102, Kerala (India); Cheruvalathu, Ajina [International School of Photonics, CUSAT, Cochin-22 (India)

    2016-05-23

    In this paper we explore the structural characterization of graphite oxide powder prepared from graphite powder by oxidation via modified Hummers method. The nonlinear optical properties of the spin coated graphite oxide thin film is also explored by open aperture Z-Scan technique. Structural and physiochemical properties of the samples were investigated with the help of Fourier Transform Infrared Spectroscopy (FTIR) and Raman Spectroscopy (Raman).The results of FT-IR and Raman spectroscopy showed that the graphite is oxidized by strong oxidants and the oxygen atoms are introduced into the graphite layers forming C=C, O-H and –C-H groups. The synthesized sample has good crystalline nature with lesser defects. The nonlinear optical property of GO thin film was studied by open aperture Z-Scan technique using Q-switched Nd-Yag Laser at 532 nm. The Z-scan plot showed that the investigated GO thin film has saturable absorption behavior. The nonlinear absorption coefficient and saturation intensity were also estimated to explore its applications in Q switched mode locking laser systems.

  8. Optically trapped nanodiamonds with nitrogen-vacancy center spins for scanning magnetometry and thermometry

    Science.gov (United States)

    Aleman, Benjamin J.; Horowitz, Viva R.; Andrich, Paolo; Christle, David J.; Toyli, David M.; Cleland, Andrew N.; Awschalom, David D.

    2013-03-01

    Nanodiamonds with nitrogen-vacancy (NV) centers are a versatile sensing platform that combines the optically addressable atom-like properties of embedded NV centers, which are sensitive to electromagnetic fields and temperature, with the physical size and mobility necessary for nanometer-scale spatial resolution. We constructed an optical tweezers apparatus that accomplishes position control of nanodiamonds in solution within a microfluidic circuit and enables simultaneous optical measurement and microwave manipulation of the NV centers' ground-state spins. We observe nanodiamond fluorescence and trapping stability over many hours, and infer high d.c. magnetic field and temperature sensitivities from measured spin resonance spectra. Scanning the position of the trapped nanodiamonds enables us to map the magnetic field of current-carrying wires and magnetic nanostructures, and perform thermometry in liquid. This work provides an approach to three-dimensional spin-based scanning probe magnetometry and thermometry in fluids for applications in the biological and physical sciences. This work was supported by AFOSR and DARPA.

  9. Transfected single-cell imaging by scanning electrochemical optical microscopy with shear force feedback regulation.

    Science.gov (United States)

    Takahashi, Yasufumi; Shiku, Hitoshi; Murata, Tatsuya; Yasukawa, Tomoyuki; Matsue, Tomokazu

    2009-12-01

    Gene-transfected single HeLa cells were characterized using a scanning electrochemical/optical microscope (SECM/OM) system with shear-force-based probe-sample distance regulation to simultaneously capture electrochemical, fluorescent, and topographic images. The outer and inner states of single living cells were obtained as electrochemical and fluorescent signals, respectively, by using an optical fiber-nanoelectrode probe. A focused ion beam (FIB) was used to mill the optical aperture and the ring electrode at the probe apex (the inner and outer radii of the ring electrode were 37 and 112 nm, respectively). To apply an appropriate shear force between the probe tip and the living cell surface, we optimized the amplitude of oscillation of the tuning fork to which the probe was attached. Field-programmable gate arrays (FPGA) were adopted to drastically increase the feedback speed of the tip-sample distance regulation, shorten the scanning time for imaging, and enhance the accuracy and quality of the living cell images. In employing these improvements, we simultaneously measured the cellular expression activity of both secreted alkaline phosphatase outside and GFP inside by using the SECM/OM with shear force distance regulation.

  10. Scanning laser optical tomography for in toto imaging of the murine cochlea.

    Directory of Open Access Journals (Sweden)

    Lena Nolte

    Full Text Available The mammalian cochlea is a complex macroscopic structure due to its helical shape and the microscopic arrangements of the individual layers of cells. To improve the outcomes of hearing restoration in deaf patients, it is important to understand the anatomic structure and composition of the cochlea ex vivo. Hitherto, only one histological technique based on confocal laser scanning microscopy and optical clearing has been developed for in toto optical imaging of the murine cochlea. However, with a growing size of the specimen, e.g., human cochlea, this technique reaches its limitations. Here, we demonstrate scanning laser optical tomography (SLOT as a valuable imaging technique to visualize the murine cochlea in toto without any physical slicing. This technique can also be applied in larger specimens up to cm3 such as the human cochlea. Furthermore, immunolabeling allows visualization of inner hair cells (otoferlin or spiral ganglion cells (neurofilament within the whole cochlea. After image reconstruction, the 3D dataset was used for digital segmentation of the labeled region. As a result, quantitative analysis of position, length and curvature of the labeled region was possible. This is of high interest in order to understand the interaction of cochlear implants (CI and cells in more detail.

  11. Adaptive distributed Kalman filtering with wind estimation for astronomical adaptive optics.

    Science.gov (United States)

    Massioni, Paolo; Gilles, Luc; Ellerbroek, Brent

    2015-12-01

    In the framework of adaptive optics (AO) for astronomy, it is a common assumption to consider the atmospheric turbulent layers as "frozen flows" sliding according to the wind velocity profile. For this reason, having knowledge of such a velocity profile is beneficial in terms of AO control system performance. In this paper we show that it is possible to exploit the phase estimate from a Kalman filter running on an AO system in order to estimate wind velocity. This allows the update of the Kalman filter itself with such knowledge, making it adaptive. We have implemented such an adaptive controller based on the distributed version of the Kalman filter, for a realistic simulation of a multi-conjugate AO system with laser guide stars on a 30 m telescope. Simulation results show that this approach is effective and promising and the additional computational cost with respect to the distributed filter is negligible. Comparisons with a previously published slope detection and ranging wind profiler are made and the impact of turbulence profile quantization is assessed. One of the main findings of the paper is that all flavors of the adaptive distributed Kalman filter are impacted more significantly by turbulence profile quantization than the static minimum mean square estimator which does not incorporate wind profile information.

  12. Lens thickness assessment: anterior segment optical coherence tomography versus A-scan ultrasonography

    Directory of Open Access Journals (Sweden)

    Nikoo Hamzeh

    2015-12-01

    Full Text Available AIM: To assess lens thickness measurements with anterior segment-optical coherence tomography (AS-OCT in comparison with A-scan ultrasonography (A-scan US. METHODS: There were 218 adult subjects (218 eyes aged 59.2±9.2y enrolled in this prospective cross-sectional study. Forty-three eyes had open angles and 175 eyes had narrow angles. Routine ophthalmic exam was performed and nuclear opacity was graded using the Lens Opacities Classification System III (LOCS III. Lens thickness was measured by AS-OCT (Visante OCT, Carl Zeiss Meditec, Dublin, CA, USA. The highest quality image was selected for each eye and lens thickness was calculated using ImageJ software. Lens thickness was also measured by A-scan US. RESULTS: Interclass correlations showed a value of 99.7% for intra-visit measurements and 95.3% for inter-visit measurements. The mean lens thickness measured by AS-OCT was not significantly different from that of A-scan US (4.861±0.404 vs 4.866±0.351 mm, P=0.74. Lens thickness values obtained from the two instruments were highly correlated overall (Pearson correlation coefficient=0.81, P<0.001, and in all LOCS III specific subgroups except in grade 5 of nuclear opacity. Bland-Altman analysis revealed a 95% limit of agreement from -0.45 to 0.46 mm. Lens thickness difference between the two instruments became smaller as the lens thickness increased and AS-OCT yielded smaller values than A-scan US in thicker lens (β=-0.29, P<0.001 CONCLUSION: AS-OCT-derived lens thickness measurement is valid and comparable to the results obtained by A-scan US. It can be used as a reliable noncontact method for measuring lens thickness in adults with or without significant cataract.

  13. Wide-field retinal optical coherence tomography with wavefront sensorless adaptive optics for enhanced imaging of targeted regions.

    Science.gov (United States)

    Polans, James; Keller, Brenton; Carrasco-Zevallos, Oscar M; LaRocca, Francesco; Cole, Elijah; Whitson, Heather E; Lad, Eleonora M; Farsiu, Sina; Izatt, Joseph A

    2017-01-01

    The peripheral retina of the human eye offers a unique opportunity for assessment and monitoring of ocular diseases. We have developed a novel wide-field (>70°) optical coherence tomography system (WF-OCT) equipped with wavefront sensorless adaptive optics (WSAO) for enhancing the visualization of smaller (23°) retina. We demonstrated the ability of our WF-OCT system to acquire non wavefront-corrected wide-field images rapidly, which could then be used to locate regions of interest, zoom into targeted features, and visualize the same region at different time points. A pilot clinical study was conducted on seven healthy volunteers and two subjects with prodromal Alzheimer's disease which illustrated the capability to image Drusen-like pathologies as far as 32.5° from the fovea in un-averaged volume scans. This work suggests that the proposed combination of WF-OCT and WSAO may find applications in the diagnosis and treatment of ocular, and potentially neurodegenerative, diseases of the peripheral retina, including diabetes and Alzheimer's disease.

  14. Characterization of Line Nanopatterns on Positive Photoresist Produced by Scanning Near-Field Optical Microscope

    Directory of Open Access Journals (Sweden)

    Sadegh Mehdi Aghaei

    2015-01-01

    Full Text Available Line nanopatterns are produced on the positive photoresist by scanning near-field optical microscope (SNOM. A laser diode with a wavelength of 450 nm and a power of 250 mW as the light source and an aluminum coated nanoprobe with a 70 nm aperture at the tip apex have been employed. A neutral density filter has been used to control the exposure power of the photoresist. It is found that the changes induced by light in the photoresist can be detected by in situ shear force microscopy (ShFM, before the development of the photoresist. Scanning electron microscope (SEM images of the developed photoresist have been used to optimize the scanning speed and the power required for exposure, in order to minimize the final line width. It is shown that nanometric lines with a minimum width of 33 nm can be achieved with a scanning speed of 75 µm/s and a laser power of 113 mW. It is also revealed that the overexposure of the photoresist by continuous wave laser generated heat can be prevented by means of proper photoresist selection. In addition, the effects of multiple exposures of nanopatterns on their width and depth are investigated.

  15. Handheld scanning probes for optical coherence tomography: developments, applications, and perspectives

    Science.gov (United States)

    Duma, V.-F.; Demian, D.; Sinescu, C.; Cernat, R.; Dobre, G.; Negrutiu, M. L.; Topala, F. I.; Hutiu, Gh.; Bradu, A.; Podoleanu, A. G.

    2016-03-01

    We present the handheld scanning probes that we have recently developed in our current project for biomedical imaging in general and for Optical Coherence Tomography (OCT) in particular. OCT is an established, but dynamic imagistic technique based on laser interferometry, which offers micrometer resolutions and millimeters penetration depths. With regard to existing devices, the newly developed handheld probes are simple, light and relatively low cost. Their design is described in detail to allow for the reproduction in any lab, including for educational purposes. Two probes are constructed almost entirely from off-the-shelf components, while a third, final variant is constructed with dedicated components, in an ergonomic design. The handheld probes have uni-dimensional (1D) galvanometer scanners therefore they achieve transversal sections through the biological sample investigated - in contrast to handheld probes equipped with bi-dimensional (2D) scanners that can also achieve volumetric (3D) reconstructions of the samples. These latter handheld probes are therefore also discussed, as well as the possibility to equip them with galvanometer 2D scanners or with Risley prisms. For galvanometer scanners the optimal scanning functions studied in a series of previous works are pointed out; these functions offer a higher temporal efficiency/duty cycle of the scanning process, as well as artifact-free OCT images. The testing of the handheld scanning probes in dental applications is presented, for metal ceramic prosthesis and for teeth.

  16. Observation of nanostructure by scanning near-field optical microscope with small sphere probe

    Directory of Open Access Journals (Sweden)

    Yasushi Oshikane, Toshihiko Kataoka, Mitsuru Okuda, Seiji Hara, Haruyuki Inoue and Motohiro Nakano

    2007-01-01

    Full Text Available Step and terrace structure has been observed in an area of 1 μm×1 μm on the cleaved surface of KCl–KBr solid-solution single crystal by scanning near-field optical microscope (SNOM with a small sphere probe of 500 nm diameter. Lateral spatial resolution of the SNOM system was estimated to be 20 nm from the observation of step width and the scanning-step interval. Vertical spatial resolution was estimated to be 5–2 nm from the observation of step height and noise level of photomultiplier tube (PMT. With applying a dielectric dipole radiation model to the probe surface, the reason why such a high spatial resolution was obtained in spite of the 500 nm sphere probe, was understood as the effect of the near-field term appeared in the radiation field equations.

  17. Z-scan determination of the third-order optical nonlinearity of gold:silica nanocomposites

    Science.gov (United States)

    Debrus, S.; Lafait, J.; May, M.; Pinçon, N.; Prot, D.; Sella, C.; Venturini, J.

    2000-10-01

    Third-order nonlinear optical properties of Au:SiO2 thin films were studied at the surface plasmon resonance wavelength by the z-scan technique using a nanosecond laser. Films were prepared by a multilayer deposition sputtering technique. They were composed of 2 nm mean diam gold particles, with a metal volume fraction of 20%. Z-scan measurements performed both with and without aperture showed a very large nonlinear absorption masking the nonlinear refraction. The nonlinear absorption coefficient β was found to be negative and equal to -1.1×10-2 cm/W. The different mechanisms contributing to this absorption are discussed and the large value of β is correlated to the duration of the laser pulses. Moreover, it is shown that a mean field theory is not appropriate to evaluate the effective susceptibility at high metal concentrations.

  18. Scanning, non-contact, hybrid broadband diffuse optical spectroscopy and diffuse correlation spectroscopy system.

    Science.gov (United States)

    Johansson, Johannes D; Mireles, Miguel; Morales-Dalmau, Jordi; Farzam, Parisa; Martínez-Lozano, Mar; Casanovas, Oriol; Durduran, Turgut

    2016-02-01

    A scanning system for small animal imaging using non-contact, hybrid broadband diffuse optical spectroscopy (ncDOS) and diffuse correlation spectroscopy (ncDCS) is presented. The ncDOS uses a two-dimensional spectrophotometer retrieving broadband (610-900 nm) spectral information from up to fifty-seven source-detector distances between 2 and 5 mm. The ncDCS data is simultaneously acquired from four source-detector pairs. The sample is scanned in two dimensions while tracking variations in height. The system has been validated with liquid phantoms, demonstrated in vivo on a human fingertip during an arm cuff occlusion and on a group of mice with xenoimplanted renal cell carcinoma.

  19. Kalman filtering to suppress spurious signals in Adaptive Optics control

    Energy Technology Data Exchange (ETDEWEB)

    Poyneer, L; Veran, J P

    2010-03-29

    In many scenarios, an Adaptive Optics (AO) control system operates in the presence of temporally non-white noise. We use a Kalman filter with a state space formulation that allows suppression of this colored noise, hence improving residual error over the case where the noise is assumed to be white. We demonstrate the effectiveness of this new filter in the case of the estimated Gemini Planet Imager tip-tilt environment, where there are both common-path and non-common path vibrations. We discuss how this same framework can also be used to suppress spatial aliasing during predictive wavefront control assuming frozen flow in a low-order AO system without a spatially filtered wavefront sensor, and present experimental measurements from Altair that clearly reveal these aliased components.

  20. Wavefront sensorless adaptive optics temporal focusing-based multiphoton microscopy

    Science.gov (United States)

    Chang, Chia-Yuan; Cheng, Li-Chung; Su, Hung-Wei; Hu, Yvonne Yuling; Cho, Keng-Chi; Yen, Wei-Chung; Xu, Chris; Dong, Chen Yuan; Chen, Shean-Jen

    2014-01-01

    Temporal profile distortions reduce excitation efficiency and image quality in temporal focusing-based multiphoton microscopy. In order to compensate the distortions, a wavefront sensorless adaptive optics system (AOS) was integrated into the microscope. The feedback control signal of the AOS was acquired from local image intensity maximization via a hill-climbing algorithm. The control signal was then utilized to drive a deformable mirror in such a way as to eliminate the distortions. With the AOS correction, not only is the axial excitation symmetrically refocused, but the axial resolution with full two-photon excited fluorescence (TPEF) intensity is also maintained. Hence, the contrast of the TPEF image of a R6G-doped PMMA thin film is enhanced along with a 3.7-fold increase in intensity. Furthermore, the TPEF image quality of 1μm fluorescent beads sealed in agarose gel at different depths is improved. PMID:24940539

  1. Performance of the Keck Observatory adaptive optics system

    Energy Technology Data Exchange (ETDEWEB)

    van Dam, M A; Mignant, D L; Macintosh, B A

    2004-01-19

    In this paper, the adaptive optics (AO) system at the W.M. Keck Observatory is characterized. The authors calculate the error budget of the Keck AO system operating in natural guide star mode with a near infrared imaging camera. By modeling the control loops and recording residual centroids, the measurement noise and band-width errors are obtained. The error budget is consistent with the images obtained. Results of sky performance tests are presented: the AO system is shown to deliver images with average Strehl ratios of up to 0.37 at 1.58 {micro}m using a bright guide star and 0.19 for a magnitude 12 star.

  2. Low-cost solar adaptive optics in the infrared

    Science.gov (United States)

    Keller, Christoph U.; Plymate, Claude; Ammons, S. M.

    2003-02-01

    We have developed a low-cost adaptive optics system for solar observations in the infrared between 1 and 28 μm with the 1.5-m McMath-Pierce solar telescope. The 37-actuator membrane mirror and a fast tip-tilt mirror are controlled by a PC running Linux RedHat 7.1 that analyzes images from a 256 by 256 pixel, 1 kHz frame rate CCD camera. The total hardware cost is less than $25,000, and the system provides diffraction-limited performance under median seeing conditions above 2.3 μm. The single Pentium III processor provides enough computing power to analyze the 200 subapertures of the Shack-Hartmann wavefront sensor in real time. We describe the hardware and software implementations and show results from the first tests at the telescope.

  3. Adaptive fiber optics collimator based on flexible hinges.

    Science.gov (United States)

    Zhi, Dong; Ma, Yanxing; Ma, Pengfei; Si, Lei; Wang, Xiaolin; Zhou, Pu

    2014-08-20

    In this manuscript, we present a new design for an adaptive fiber optics collimator (AFOC) based on flexible hinges by using piezoelectric stacks actuators for X-Y displacement. Different from traditional AFOC, the new structure is based on flexible hinges to drive the fiber end cap instead of naked fiber. We fabricated a real AFOC based on flexible hinges, and the end cap's deviation and resonance frequency of the device were measured. Experimental results show that this new AFOC can provide fast control of tip-tilt deviation of the laser beam emitting from the end cap. As a result, the fiber end cap can support much higher power than naked fiber, which makes the new structure ideal for tip-tilt controlling in a high-power fiber laser system.

  4. Method for Compensation of Eye Movements in Adaptive Optics

    Science.gov (United States)

    Karitans, V.; Ozolinsh, M.

    2010-03-01

    The benefit of correction of aberrations in the human eye with adaptive optics is strongly reduced by eye movements. Although devices for compensation of eye movements have already been developed, these are very advanced and expensive. We are working on the development of a novel method for eye movement compensation based on the detection of a corneal reflex by a linear profile sensor. Electronic circuits for controlling the profile sensor and stepper motors have already been designed. Next, a circuit must be designed so that the profile sensor controls the stepper motor. One of the shortcomings of this method is that a stepper motor runs at low speed. Another shortcoming is that the profile sensor operates in only one dimension. However, these problems can be solved by using a two-dimensional profile sensor and piezo-mounts working at much higher frequencies.

  5. Focus scanning with feedback control for fiber-optic nonlinear endomicroscopy (Conference Presentation)

    Science.gov (United States)

    Li, Ang; Liang, Wenxuan; Li, Xingde

    2017-02-01

    Fiber-optic nonlinear endomicroscopy represents a strong promise to enable translation of nonlinear microscopy technologies to in vivo applications, particularly imaging of internal organs. Two-dimensional imaging beam scanning has been accomplished by using fiber-optic scanners or MEMS scanners. Yet nonlinear endomicroscopy still cannot perform rapid and reliable depth or focus scanning while maintaining a small form factor. Shape memory alloy (SMA) wire had shown promise in extending 2D endoscopic imaging to the third dimension. By Joule heating, the SMA wire would contract and move the endomicroscope optics to change beam focus. However, this method suffered from hysteresis, and was susceptible to change in ambient temperature, making it difficult to achieve accurate and reliable depth scanning. Here we present a feedback-controlled SMA actuator which addressed these challenges. The core of the feedback loop was a Hall effect sensor. By measuring the magnetic flux density from a tiny magnet attached to the SMA wire, contraction distance of the SMA wire could be tracked in real time. The distance was then fed to the PID algorithm running in a microprocessor, which computed the error between the command position and the current position of the actuator. The current running through the SMA wire was adjusted accordingly. Our feedback-controlled SMA actuator had a tube-like shape with outer diameter of 5.5 mm and length of 25 mm, and was designed to house the endomicroscope inside. Initial test showed that it allowed more than 300 microns of travel distance, with an average positioning error of less than 2 microns. 3D imaging experiments with the endomicroscope is underway, and its imaging performance will be assessed and discussed.

  6. Implementation of 3D prostrate ring-scanning mechanism for NIR diffuse optical imaging phantom validation

    Science.gov (United States)

    Yu, Jhao-Ming; Chen, Liang-Yu; Pan, Min-Cheng; Hsu, Ya-Fen; Pan, Min-Chun

    2015-03-01

    Diffuse optical imaging (DOI) providing functional information of tissues has drawn great attention for the last two decades. Near infrared (NIR) DOI systems composed of scanning bench, opt-electrical measurement module, system control, and data processing and image reconstruction schemes are developed for the screening and diagnosis of breast tumors. Mostly, the scanning bench belonging to fixed source-and-detector configuration limits computed image resolution to an extent. To cope with the issue, we propose, design and implement a 3D prostrate ring-scanning equipment for NIR DOI with flexible combinations of illumination and detection, and with the function of radial, circular and vertical movement without hard compression of breast tissue like the imaging system using or incorporating with X-ray mammographic bench. Especially, a rotation-sliding-and-moving mechanism was designed for the guidance of source- and detection-channel movement. Following the previous justification for synthesized image reconstruction, in the paper the validation using varied phantoms is further conducted and 3D image reconstruction for their absorption and scattering coefficients is illustrated through the computation of our in-house coded schemes. The source and detection NIR data are acquired to reconstruct the 3D images through the operation of scanning bench in the movement of vertical, radial and circular directions. Rather than the fixed configuration, the addressed screening/diagnosing equipment has the flexibility for optical-channel expansion with a compromise among construction cost, operation time, and spatial resolution of reconstructed μa and μs' images.

  7. Phase stabilized homodyne of infrared scattering type scanning near-field optical microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Xiaoji G., E-mail: xgx214@lehigh.edu [Department of Chemistry, Lehigh University, Bethlehem, Pennsylvania 18015 (United States); Gilburd, Leonid; Walker, Gilbert C., E-mail: gwalker@chem.utoronto.ca [Department of Chemistry, University of Toronto, Toronto, Ontario M5S 3H6 (Canada)

    2014-12-29

    Scattering type scanning near-field optical microscopy (s-SNOM) allows sub diffraction limited spatial resolution. Interferometric homodyne detection in s-SNOM can amplify the signal and extract vibrational responses based on sample absorption. A stable reference phase is required for a high quality homodyne-detected near-field signal. This work presents the development of a phase stabilization mechanism for s-SNOM to provide stable homodyne conditions. The phase stability is found to be better than 0.05 rad for the mid infrared light source. Phase stabilization results in improved near field images and vibrational spectroscopies. Spatial inhomogeneities of the boron nitride nanotubes are measured and compared.

  8. Tuning Localized Surface Plasmon Resonance in Scanning Near-Field Optical Microscopy Probes.

    Science.gov (United States)

    Vasconcelos, Thiago L; Archanjo, Bráulio S; Fragneaud, Benjamin; Oliveira, Bruno S; Riikonen, Juha; Li, Changfeng; Ribeiro, Douglas S; Rabelo, Cassiano; Rodrigues, Wagner N; Jorio, Ado; Achete, Carlos A; Cançado, Luiz Gustavo

    2015-06-23

    A reproducible route for tuning localized surface plasmon resonance in scattering type near-field optical microscopy probes is presented. The method is based on the production of a focused-ion-beam milled single groove near the apex of electrochemically etched gold tips. Electron energy-loss spectroscopy and scanning transmission electron microscopy are employed to obtain highly spatially and spectroscopically resolved maps of the milled probes, revealing localized surface plasmon resonance at visible and near-infrared wavelengths. By changing the distance L between the groove and the probe apex, the localized surface plasmon resonance energy can be fine-tuned at a desired absorption channel. Tip-enhanced Raman spectroscopy is applied as a test platform, and the results prove the reliability of the method to produce efficient scattering type near-field optical microscopy probes.

  9. 4th International Workshop on Adaptive Optics for Industry and Medicine

    CERN Document Server

    Wittrock, Ulrich

    2005-01-01

    This book treats the development and application of adaptive optics for industry and medicine. The contributions describe recently developed components for adaptive-optics systems such as deformable mirrors, wavefront sensors, and mirror drivers as well as complete adaptive optical systems and their applications in industry and medicine. Applications range from laser-beam forming and adaptive aberration correction for high-power lasers to retinal imaging in ophthalmology. The contributions are based on presentations made at the 4th International Workshop on Adaptive Optics in Industry and Medicine which took place in Münster, Germany, in October 2003. This highly successful series of workshops on adaptive optics started in 1997 and continues with the 5th workshop in Beijing in 2005.

  10. Guided access cavity preparation using cone-beam computed tomography and optical surface scans - an ex vivo study

    DEFF Research Database (Denmark)

    Buchgreitz, J; Buchgreitz, M; Mortensen, D

    2016-01-01

    AIM: To evaluate ex vivo, the accuracy of a preparation procedure planned for teeth with pulp canal obliteration (PCO) using a guide rail concept based on a cone-beam computed tomography (CBCT) scan merged with an optical surface scan. METHODOLOGY: A total of 48 teeth were mounted in acrylic bloc...

  11. A Bayesian regularized artificial neural network for adaptive optics forecasting

    Science.gov (United States)

    Sun, Zhi; Chen, Ying; Li, Xinyang; Qin, Xiaolin; Wang, Huiyong

    2017-01-01

    Real-time adaptive optics is a technology for enhancing the resolution of ground-based optical telescopes and overcoming the disturbance of atmospheric turbulence. The performance of the system is limited by delay errors induced by the servo system and photoelectrons noise of wavefront sensor. In order to cut these delay errors, this paper proposes a novel model to forecast the future control voltages of the deformable mirror. The predictive model is constructed by a multi-layered back propagation network with Bayesian regularization (BRBP). For the purpose of parallel computation and less disturbance, we adopt a number of sub-BP neural networks to substitute the whole network. The Bayesian regularized network assigns a probability to the network weights, allowing the network to automatically and optimally penalize excessively complex models. The simulation results show that the BRBP introduces smaller mean absolute percentage error (MAPE) and mean square errors (MSE) than other typical algorithms. Meanwhile, real data analysis results show that the BRBP model has strong generalization capability and parallelism.

  12. Adaptive compressed sensing for spectral-domain optical coherence tomography

    Science.gov (United States)

    Wang, Yi; Chen, Xiaodong; Wang, Ting; Li, Hongxiao; Yu, Daoyin

    2014-03-01

    Spectral-domain optical coherence tomography (SD-OCT) is a non-contact and non-invasive method for measuring the change of biological tissues caused by pathological changes of body. CCD with huge number of pixels is usually used in SD-OCT to increase the detecting depth, thus enhancing the hardness of data transmission and storage. The usage of compressed sensing (CS) in SD-OCT is able to reduce the trouble of large data transfer and storage, thus eliminating the complexity of processing system. The traditional CS uses the same sampling model for SD-OCT images of different tissue, leading to reconstruction images with different quality. We proposed a CS with adaptive sampling model. The new model is based on uniform sampling model, and the interference spectral of SD-OCT is considered to adjust the local sampling ratio. Compared with traditional CS, adaptive CS can modify the sampling model for images of different tissue according to different interference spectral, getting reconstruction images with high quality without changing sampling model.

  13. Design and testing of prototype handheld scanning probes for optical coherence tomography.

    Science.gov (United States)

    Demian, Dorin; Duma, Virgil-Florin; Sinescu, Cosmin; Negrutiu, Meda Lavinia; Cernat, Ramona; Topala, Florin Ionel; Hutiu, Gheorghe; Bradu, Adrian; Podoleanu, Adrian Gh

    2014-08-01

    Three simple and low-cost configurations of handheld scanning probes for optical coherence tomography have been developed. Their design and testing for dentistry applications are presented. The first two configurations were built exclusively from available off-the-shelf optomechanical components, which, to the best of our knowledge, are the first designs of this type. The third configuration includes these components in an optimized and ergonomic probe. All the designs are presented in detail to allow for their duplication in any laboratory with a minimum effort, for applications that range from educational to high-end clinical investigations. Requirements that have to be fulfilled to achieve configurations which are reliable, ergonomic-for clinical environments, and easy to build are presented. While a range of applications is possible for the prototypes developed, in this study the handheld probes are tested ex vivo with a spectral domain optical coherence tomography system built in-house, for dental constructs. A previous testing with a swept source optical coherence tomography system has also been performed both in vivo and ex vivo for ear, nose, and throat-in a medical environment. The applications use the capability of optical coherence tomography to achieve real-time, high-resolution, non-contact, and non-destructive interferometric investigations with micrometer resolutions and millimeter penetration depth inside the sample. In this study, testing the quality of the material of one of the most used types of dental prosthesis, metalo-ceramic is thus demonstrated. © IMechE 2014.

  14. [Specifity of optic disc evaluation in healthy subjects with large optic discs and physiologic cupping using confocal scanning laser ophthalmoscopy].

    Science.gov (United States)

    Plange, N; Hirsch, T; Bienert, M; Remky, A

    2014-02-01

    Imaging methods of the optic nerve head appear to have an increasing impact in glaucoma diagnosis. The aim of this study is to evaluate the specifity of the Heidelberg Retina Tomograph (software version 1.7 and 3.0) in subjects with physiological cupping and large optic discs. 27 eyes of 27 subjects (mean age 41.3 ± 15.8 years) with bilateral physiological cupping and large optic discs (vertical cup-to-disc-ratio > 0.3, optic disc area 2.48 ± 0.45 mm2, max. 3.54 mm2) were included in a clinical study. All subjects had an intraocular pressure cupping by funduscopy and no nerve fibre layer defects (Scanning Laser Ophthalmoscope, Rodenstock, Germany). Standard achromatic perimetry (Humphrey Field Analyzer, Humphrey-Zeiss, 24/2 SITA or full threshold), short-wavelength automated perimetry (Humphrey Field Analyzer, Humphrey-Zeiss), and frequency doubling technology (FDT, Humphrey-Zeiss) revealed no visual field defects. Optic disc imaging was performed in all subjects using the Heidelberg Retina Tomograph II (HRT). Optic disc images were transferred to the software-update of the HRT 3 (Version 3.0, Heidelberg Engineering). Specifity was calculated using the Moorfields regression analysis (MRA, software version 1.7 and 3.0) and the glaucoma probability score (GPS analysis) using all disc sectors and omitting the nasal and 3 nasal sectors. Specifity of the MRA (software version 1.7) was 66.6 % (most specific criteria), and 22.2 % (least specific criteria). Specifity of the MRA (software version 3.0) was 33.3 % (most specific criteria), and 14.8 % (least specific criteria), whereas specifity of the GPS analysis was 37.0 % (most specific criteria), and 11.1 % (least specific criteria). When the nasal sectors were omitted for analysis, specifity increased for the MRA analysis, but not for the GPS analysis. Specifity of the MRA was unsatisfactory using the software version 1.7 and 3.0 in subjects with large optic discs and physiological cupping

  15. Phase-resolved optical coherence tomography and optical Doppler tomography for imaging blood flow in human skin with fast scanning speed and high velocity sensitivity

    NARCIS (Netherlands)

    Zhao, YH; Chen, ZP; Saxer, CE; Xiang, SH; de Boer, JF; Nelson, JS

    2000-01-01

    We have developed a novel phase-resolved optical coherence tomography (OCT) and optical Doppler tomography (ODT) system that uses phase information derived from a Hilbert transformation to image blood flow in human skin with fast scanning speed and high velocity sensitivity. Using the phase change

  16. Optical photon reassignment super-resolved scanning laser ophthalmoscopy (Conference Presentation)

    Science.gov (United States)

    LaRocca, Francesco; DuBose, Theodore B.; Farsiu, Sina; Izatt, Joseph A.

    2017-02-01

    Conventional scanning laser ophthalmoscopy (SLO) utilizes a finite collection pinhole at a retinal conjugate plane to strongly reject out-of-focus light while primarily transmitting the in-focus, retinal backscattered signal. However, to improve lateral resolution, a sub-Airy disk collection pinhole is necessary, which drastically reduces the signal-to-noise ratio (SNR) of the system and is thus not commonly employed. Recently, an all-optical, super-resolution microscopy technique known as optical photon reassignment (OPRA) microscopy (also known as re-scan confocal microscopy) has been developed to bypass this fundamental tradeoff between resolution and SNR in confocal microscopy. We present a methodology and system design for obtaining super resolution in retinal imaging by combining the concepts of SLO and OPRA microscopy. The resolution improvement of the system was quantified using a 1951 USAF target at a telecentric intermediate image plane. Retinal images from human volunteers were acquired with this system both with and without using the OPRA technique to demonstrate the resolution improvement when imaging parafoveal cone photoreceptors. Finally, we quantified the resolution improvement in the retina by analyzing the radially averaged power spectrum of the retinal images.

  17. Development of apertureless near-field scanning optical microscope tips for tip-enhanced Raman spectroscopy.

    Science.gov (United States)

    Kodama, T; Umezawa, T; Watanabe, S; Ohtani, H

    2008-02-01

    In this study, we suggested two types of novel metallized tip for the apertureless near-field scanning optical microscope probe. The first is a silver nanorod immobilized tip and the other is a double metallized probe. We calculated the electric field enhancement factor and the electric field distribution of a single sphere, aggregated spheres, an ellipse and a nanorod by the finite-differential time-domain method to improve the silver nanosphere immobilized tip developed in our previous studies. The enhanced field of the nanorod is localized at the external surfaces. The simulation results of the nanorod revealed that the position of the maximum peak is shifted to a longer wavelength and that its electric field enhancement factor increases as the aspect ratio increases. Thus, we developed the silver nanorod immobilized tip, and the tip-enhanced Raman spectrum of rhodamine 6G molecule on the substrate could be measured by the tip though it could not be detected by the previous nanosphere immobilized tip. Further, the finite-differential time-domain calculation predicted that the double metallized tips considerably enhance the electric field and that its enhancement factor in the longer wavelength region (500-600 nm) does not decrease when the tip is rounded. The results show that the proposed metallized tips were useful for the apertureless near-field scanning optical microscope system.

  18. Acousto-optically scanned video-rate image dissector tube confocal microscope suitable for use with multiple wavelengths

    Science.gov (United States)

    Hubin, Thomas; Goldstein, Seth R.; Smith, Thomas G., Jr.

    1994-04-01

    We will present the design of a greatly simplified acousto-optic (A/O) scanning system which allows a change of wavelength in less than a second (and in principal between 2 TV lines). A/O deflectors now available with a 9.3 mm circular entrance pupil (rather than the 2 mm X 12 mm pupils previously used) eliminate the need for costly anamorphic beam shaping optics. The resulting simplified optic system can be straightforwardly corrected for different excitation wavelengths.

  19. Modeling a Miniaturized Scanning Electron Microscope Focusing Column - Lessons Learned in Electron Optics Simulation

    Science.gov (United States)

    Loyd, Jody; Gregory, Don; Gaskin, Jessica

    2016-01-01

    This presentation discusses work done to assess the design of a focusing column in a miniaturized Scanning Electron Microscope (SEM) developed at the NASA Marshall Space Flight Center (MSFC) for use in-situ on the Moon-in particular for mineralogical analysis. The MSFC beam column design uses purely electrostatic fields for focusing, because of the severe constraints on mass and electrical power consumption imposed by the goals of lunar exploration and of spaceflight in general. The resolution of an SEM ultimately depends on the size of the focused spot of the scanning beam probe, for which the stated goal here is a diameter of 10 nanometers. Optical aberrations are the main challenge to this performance goal, because they blur the ideal geometrical optical image of the electron source, effectively widening the ideal spot size of the beam probe. In the present work the optical aberrations of the mini SEM focusing column were assessed using direct tracing of non-paraxial rays, as opposed to mathematical estimates of aberrations based on paraxial ray-traces. The geometrical ray-tracing employed here is completely analogous to ray-tracing as conventionally understood in the realm of photon optics, with the major difference being that in electron optics the lens is simply a smoothly varying electric field in vacuum, formed by precisely machined electrodes. Ray-tracing in this context, therefore, relies upon a model of the electrostatic field inside the focusing column to provide the mathematical description of the "lens" being traced. This work relied fundamentally on the boundary element method (BEM) for this electric field model. In carrying out this research the authors discovered that higher accuracy in the field model was essential if aberrations were to be reliably assessed using direct ray-tracing. This led to some work in testing alternative techniques for modeling the electrostatic field. Ultimately, the necessary accuracy was attained using a BEM

  20. Three-dimensional phase-contrast X-ray microtomography with scanning-imaging X-ray microscope optics.

    Science.gov (United States)

    Takeuchi, Akihisa; Uesugi, Kentaro; Suzuki, Yoshio

    2013-09-01

    A three-dimensional (3D) X-ray tomographic micro-imaging system has been developed. The optical system is based on a scanning-imaging X-ray microscope (SIXM) optics, which is a hybrid system consisting of a scanning microscope optics with a one-dimensional (1D) focusing (line-focusing) device and an imaging microscope optics with a 1D objective. In the SIXM system, each 1D dataset of a two-dimensional (2D) image is recorded independently. An object is illuminated with a line-focused beam. Positional information of the region illuminated by the line-focused beam is recorded with the 1D imaging microscope optics as line-profile data. By scanning the object with the line focus, 2D image data are obtained. In the same manner as for a scanning microscope optics with a multi-pixel detector, imaging modes such as phase contrast and absorption contrast can be arbitrarily configured after the image data acquisition. By combining a tomographic scan method and the SIXM system, quantitative 3D imaging is performed. Results of a feasibility study of the SIXM for 3D imaging are shown.

  1. Modelling MEMS deformable mirrors for astronomical adaptive optics

    Science.gov (United States)

    Blain, Celia

    As of July 2012, 777 exoplanets have been discovered utilizing mainly indirect detection techniques. The direct imaging of exoplanets is the next goal for astronomers, because it will reveal the diversity of planets and planetary systems, and will give access to the exoplanet's chemical composition via spectroscopy. With this spectroscopic knowledge, astronomers will be able to know, if a planet is terrestrial and, possibly, even find evidence of life. With so much potential, this branch of astronomy has also captivated the general public attention. The direct imaging of exoplanets remains a challenging task, due to (i) the extremely high contrast between the parent star and the orbiting exoplanet and (ii) their small angular separation. For ground-based observatories, this task is made even more difficult, due to the presence of atmospheric turbulence. High Contrast Imaging (HCI) instruments have been designed to meet this challenge. HCI instruments are usually composed of a coronagraph coupled with the full onaxis corrective capability of an Extreme Adaptive Optics (ExAO) system. An efficient coronagraph separates the faint planet's light from the much brighter starlight, but the dynamic boiling speckles, created by the stellar image, make exoplanet detection impossible without the help of a wavefront correction device. The Subaru Coronagraphic Extreme Adaptive Optics (SCExAO) system is a high performance HCI instrument developed at Subaru Telescope. The wavefront control system of SCExAO consists of three wavefront sensors (WFS) coupled with a 1024- actuator Micro-Electro-Mechanical-System (MEMS) deformable mirror (DM). MEMS DMs offer a large actuator density, allowing high count DMs to be deployed in small size beams. Therefore, MEMS DMs are an attractive technology for Adaptive Optics (AO) systems and are particularly well suited for HCI instruments employing ExAO technologies. SCExAO uses coherent light modulation in the focal plane introduced by the DM, for

  2. Live endothelial cells imaged by Scanning Near-field Optical Microscopy (SNOM): capabilities and challenges.

    Science.gov (United States)

    Bulat, Katarzyna; Rygula, Anna; Szafraniec, Ewelina; Ozaki, Yukihiro; Baranska, Malgorzata

    2017-06-01

    The scanning near-field optical microscopy (SNOM) shows a potential to study details of biological samples, since it provides the optical images of objects with nanometric spatial resolution (50-200 nm) and the topographic information at the same time. The goal of this work is to demonstrate the capabilities of SNOM in transmission configuration to study human endothelial cells and their morphological changes, sometimes very subtle, upon inflammation. Various sample preparations were tested for SNOM measurements and promising results are collected to show: 1) the influence of α tumor necrosis factor (TNF-α) on EA.hy 926 cells (measurements of the fixed cells); 2) high resolution images of various endothelial cell lines, i.e. EA.hy 926 and HLMVEC (investigations of the fixed cells in buffer environment); 3) imaging of live endothelial cells in physiological buffers. The study demonstrate complementarity of the SNOM measurements performed in air and in liquid environments, on fixed as well as on living cells. Furthermore, it is proved that the SNOM is a very useful method for analysis of cellular morphology and topography. Changes in the cell shape and nucleus size, which are the symptoms of inflammatory reaction, were noticed in TNF-α activated EA.hy 926 cells. The cellular structures of submicron size were observed in high resolution optical images of cells from EA.hy 926 and HLMVEC lines. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Assessment of the Optic Disc Morphology Using Spectral-Domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

    Directory of Open Access Journals (Sweden)

    Pilar Calvo

    2014-01-01

    Full Text Available Objective. To compare the equivalent optic nerve head (OHN parameters obtained with confocal scanning laser ophthalmoscopy (HRT3 and spectral-domain optical coherence tomography (OCT in healthy and glaucoma patients. Methods. One hundred and eighty-two consecutive healthy subjects and 156 patients with open-angle glaucoma were divided into 2 groups according to intraocular pressure and visual field outcomes. All participants underwent imaging of the ONH with the HRT3 and the Cirrus OCT. The ONH parameters and the receiver operating characteristic (ROC curves were compared between both groups. Results. Mean age did not differ between the normal and glaucoma groups (59.55 ± 9.7 years and 61.05 ± 9.4 years, resp.; P=0.15. Rim area, average cup-to-disc (C/D ratio, vertical C/D ratio, and cup volume were different between both instruments (P<0.001. All equivalent ONH parameters, except disc area, were different between both groups (P<0.001. The best areas under the ROC curve were observed for vertical C/D ratio (0.980 for OCT and 0.942 for HRT3; P=0.11. Sensitivities at 95% fixed-specificities of OCT parameters were higher than those of HRT3. Conclusions. Equivalent ONH parameters of Cirrus OCT and HRT3 are different and cannot be used interchangeably. ONH parameters measured with OCT yielded a slightly better diagnostic performance.

  4. Real-time polarization mode dispersion monitoring system for a multiple-erbium-doped fiber amplifier, dense wavelength division multiplexing optical fiber transmission by amplified spontaneous emission modulation and acousto-optic tunable fiber scanning techniques.

    Science.gov (United States)

    Tseng, Bao-Jang; Tarn, Chen-Wen

    2009-03-01

    Without interruption or affecting the transmission of ordinary payload channels, we propose a real time polarization mode dispersion (PMD) monitoring system for long-haul, multiple erbium-doped fiber amplifier (EDFA), dense wavelength division multiplexing (DWDM) optical fiber transmission using modulated amplified spontaneous emission (ASE) of one of the EDFAs as the supervisory (SV) signal source. An acousto-optic tunable filter (AOTF) at the receiver side is adopted to scan the spectrum of the transmitted ASE SV signal. Using the fixed-analyzer method, PMDs of different wavelength bands that range from 1545 to 1580 nm of a DWDM fiber-optic communication system can be found by adaptively changing the radio frequency of the AOTF. The resolution and the measuring range of the proposed monitoring system can be significantly improved by cascading the AOTFs at the receiver side.

  5. Review of adaptive optics OCT (AO-OCT): principles and applications for retinal imaging [Invited].

    Science.gov (United States)

    Pircher, Michael; Zawadzki, Robert J

    2017-05-01

    In vivo imaging of the human retina with a resolution that allows visualization of cellular structures has proven to be essential to broaden our knowledge about the physiology of this precious and very complex neural tissue that enables the first steps in vision. Many pathologic changes originate from functional and structural alterations on a cellular scale, long before any degradation in vision can be noted. Therefore, it is important to investigate these tissues with a sufficient level of detail in order to better understand associated disease development or the effects of therapeutic intervention. Optical retinal imaging modalities rely on the optical elements of the eye itself (mainly the cornea and lens) to produce retinal images and are therefore affected by the specific arrangement of these elements and possible imperfections in curvature. Thus, aberrations are introduced to the imaging light and image quality is degraded. To compensate for these aberrations, adaptive optics (AO), a technology initially developed in astronomy, has been utilized. However, the axial sectioning provided by retinal AO-based fundus cameras and scanning laser ophthalmoscope instruments is limited to tens of micrometers because of the rather small available numerical aperture of the eye. To overcome this limitation and thus achieve much higher axial sectioning in the order of 2-5µm, AO has been combined with optical coherence tomography (OCT) into AO-OCT. This enabled for the first time in vivo volumetric retinal imaging with high isotropic resolution. This article summarizes the technical aspects of AO-OCT and provides an overview on its various implementations and some of its clinical applications. In addition, latest developments in the field, such as computational AO-OCT and wavefront sensor less AO-OCT, are covered.

  6. Review of adaptive optics OCT (AO-OCT): principles and applications for retinal imaging [Invited

    Science.gov (United States)

    Pircher, Michael; Zawadzki, Robert J

    2017-01-01

    In vivo imaging of the human retina with a resolution that allows visualization of cellular structures has proven to be essential to broaden our knowledge about the physiology of this precious and very complex neural tissue that enables the first steps in vision. Many pathologic changes originate from functional and structural alterations on a cellular scale, long before any degradation in vision can be noted. Therefore, it is important to investigate these tissues with a sufficient level of detail in order to better understand associated disease development or the effects of therapeutic intervention. Optical retinal imaging modalities rely on the optical elements of the eye itself (mainly the cornea and lens) to produce retinal images and are therefore affected by the specific arrangement of these elements and possible imperfections in curvature. Thus, aberrations are introduced to the imaging light and image quality is degraded. To compensate for these aberrations, adaptive optics (AO), a technology initially developed in astronomy, has been utilized. However, the axial sectioning provided by retinal AO-based fundus cameras and scanning laser ophthalmoscope instruments is limited to tens of micrometers because of the rather small available numerical aperture of the eye. To overcome this limitation and thus achieve much higher axial sectioning in the order of 2-5µm, AO has been combined with optical coherence tomography (OCT) into AO-OCT. This enabled for the first time in vivo volumetric retinal imaging with high isotropic resolution. This article summarizes the technical aspects of AO-OCT and provides an overview on its various implementations and some of its clinical applications. In addition, latest developments in the field, such as computational AO-OCT and wavefront sensor less AO-OCT, are covered. PMID:28663890

  7. Control code for laboratory adaptive optics teaching system

    Science.gov (United States)

    Jin, Moonseob; Luder, Ryan; Sanchez, Lucas; Hart, Michael

    2017-09-01

    By sensing and compensating wavefront aberration, adaptive optics (AO) systems have proven themselves crucial in large astronomical telescopes, retinal imaging, and holographic coherent imaging. Commercial AO systems for laboratory use are now available in the market. One such is the ThorLabs AO kit built around a Boston Micromachines deformable mirror. However, there are limitations in applying these systems to research and pedagogical projects since the software is written with limited flexibility. In this paper, we describe a MATLAB-based software suite to interface with the ThorLabs AO kit by using the MATLAB Engine API and Visual Studio. The software is designed to offer complete access to the wavefront sensor data, through the various levels of processing, to the command signals to the deformable mirror and fast steering mirror. In this way, through a MATLAB GUI, an operator can experiment with every aspect of the AO system's functioning. This is particularly valuable for tests of new control algorithms as well as to support student engagement in an academic environment. We plan to make the code freely available to the community.

  8. Deblurring adaptive optics retinal images using deep convolutional neural networks.

    Science.gov (United States)

    Fei, Xiao; Zhao, Junlei; Zhao, Haoxin; Yun, Dai; Zhang, Yudong

    2017-12-01

    The adaptive optics (AO) can be used to compensate for ocular aberrations to achieve near diffraction limited high-resolution retinal images. However, many factors such as the limited aberration measurement and correction accuracy with AO, intraocular scatter, imaging noise and so on will degrade the quality of retinal images. Image post processing is an indispensable and economical method to make up for the limitation of AO retinal imaging procedure. In this paper, we proposed a deep learning method to restore the degraded retinal images for the first time. The method directly learned an end-to-end mapping between the blurred and restored retinal images. The mapping was represented as a deep convolutional neural network that was trained to output high-quality images directly from blurry inputs without any preprocessing. This network was validated on synthetically generated retinal images as well as real AO retinal images. The assessment of the restored retinal images demonstrated that the image quality had been significantly improved.

  9. Novel adaptive fiber-optics collimator for coherent beam combination.

    Science.gov (United States)

    Zhi, Dong; Ma, Pengfei; Ma, Yanxing; Wang, Xiaolin; Zhou, Pu; Si, Lei

    2014-12-15

    In this manuscript, we experimentally validate a novel design of adaptive fiber-optics collimator (AFOC), which utilizes two levers to enlarge the movable range of the fiber end cap. The enlargement of the range makes the new AFOC possible to compensate the end-cap/tilt aberration in fiber laser beam combining system. The new AFOC based on flexible hinges and levers was fabricated and the performance of the new AFOC was tested carefully, including its control range, frequency response and control accuracy. Coherent beam combination (CBC) of two 5-W fiber amplifiers array with simultaneously end-cap/tilt control and phase-locking control was implemented successfully with the novel AFOC. Experimental results show that the average normalized power in the bucket (PIB) value increases from 0.311 to 0.934 with active phasing and tilt aberration compensation simultaneously, and with both controls on, the fringe contrast improves to more than 82% from 0% for the case with both control off. This work presents a promising structure for tilt aberration control in high power CBC system.

  10. [Reproducibility of goniometry with slitlamp-adapted optical coherence tomography].

    Science.gov (United States)

    Karandish, A; Wirbelauer, C; Häberle, H; Pham, D T

    2004-06-01

    Visualization of the anterior chamber angle (ACA) is an important diagnostic part of evaluating patients with glaucoma. The purpose of this study was to evaluate the intra- and interobserver variability and reliability of the ACA and angle opening distance (AOD) measurements using optical coherence tomography (OCT). To evaluate the intra- and interobserver variability, ACA and AOD were both measured five times and in three consecutive images in 22 patients (24 eyes) by two experienced observers. The intraclass correlation coefficient (ICC) as a measure of reliability was determined to estimate the intra- and interobserver variability. The main outcome measures were accuracy, reproducibility assessed with the coefficient of variation (CV), and the limits of agreement of ACA and AOD. The intraobserver variability of five replicate measurements was +/-1.4 degrees for ACA (CV 6.2%) and +/-11 micro m for AOD (CV 4%). The ICC for the intraobserver reliability was 0.99 for both ACA and AOD. The interobserver variability of three intersessional measurements was +/-2.5 degrees for ACA (CV 10.9%) and +/-24 micro m (CV 8.3%) for AOD. The ICC was 0.95 for ACA and 0.98 for AOD. There was no difference ( p>0.05) between the two observers measuring ACA and AOD. Two-dimensional visualization of the ACA and its assessment with slitlamp-adapted OCT yielded reliable ACA and AOD measurements in a clinical setting. Thus, OCT goniometry could provide an objective method to assess the anterior chamber angle.

  11. Packetisation in Optical Packet Switch Fabrics using adaptive timeout values

    DEFF Research Database (Denmark)

    Mortensen, Brian Bach

    2006-01-01

    Hybrid electro-optical packet switches utilize optics in the backplane to switch optical packets from inputs to outputs on electronic line cards. The optical packets are traditionally considerably larger than minimum size IP packets. IP packets entering the switch must be formatted (segmented...... delay and throughput in hybrid electro-optical packet switching. Furthermore, it is investigated how large a speedup is required in order to provide 100% throughput....

  12. Comparison of the marginal adaptation of direct and indirect composite inlay restorations with optical coherence tomography

    Directory of Open Access Journals (Sweden)

    Ayşe Gözde TÜRK

    Full Text Available ABSTRACT Objective The purpose of the study was to use the photonic imaging modality of optical coherence tomography (OCT to compare the marginal adaptation of composite inlays fabricated by direct and indirect techniques. Material and Methods Class II cavities were prepared on 34 extracted human molar teeth. The cavities were randomly divided into two groups according to the inlay fabrication technique. The first group was directly restored on cavities with a composite (Esthet X HD, Dentsply, Germany after isolating. The second group was indirectly restored with the same composite material. Marginal adaptations were scanned before cementation with an invisible infrared light beam of OCT (Thorlabs, allowing measurement in 200 µm intervals. Restorations were cemented with a self-adhesive cement resin (SmartCem2, Dentsply, and then marginal adaptations were again measured with OCT. Mean values were statistically compared by using independent-samples t-test and paired samples t-test (p<0.05, before and after cementation. Results Direct inlays presented statistically smaller marginal discrepancy values than indirect inlays, before (p=0.00001442 and after (p=0.00001466 cementation. Marginal discrepancy values were increased for all restorations after cementation (p=0.00008839, p=0.000000952 for direct and indirect inlays, respectively. The mean marginal discrepancy value of the direct group increased from 56.88±20.04 µm to 91.88±31.7 µm, whereas the indirect group increased from 107.54±35.63 µm to 170.29±54.83 µm. Different techniques are available to detect marginal adaptation of restorations, but the OCT system can give quantitative information about resin cement thickness and its interaction between tooth and restoration in a nondestructive manner. Conclusions Direct inlays presented smaller marginal discrepancy than indirect inlays. The marginal discrepancy values were increased for all restorations that refer to cement thickness after

  13. Modular multimodal swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography scan-head for surgical microscope-integrated and slit-lamp imaging

    Science.gov (United States)

    Malone, Joseph D.; Li, Jianwei D.; El-Haddad, Mohamed T.; Joos, Karen M.; Patel, Shriji N.; Tao, Yuankai K.

    2017-02-01

    Scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) enable noninvasive in vivo diagnostic imaging and provide complementary en face and depth-resolved visualization of ophthalmic structures, respectively. We previously demonstrated concurrent multimodal swept-source spectrally encoded scanning laser ophthalmoscopy and OCT (SS-SESLO-OCT) at 1060 nm using a swept-source and double clad fiber coupler. Here, we present system enhancements and novel designs for a modular SS-SESLO-OCT scan-head that can be coupled to ophthalmic surgical microscope-integrated and slit-lamp imaging optics. Multimodal SS-SESLO-OCT was demonstrated using a custom-built swept-source OCT engine with a 200 kHz 1060 nm source that was optically buffered for concurrent SESLO and OCT imaging at 100% duty cycle and 400 kHz sweep-rate. A shared optical relay and fast-axis galvanometer ensured inherent co-registration between SESLO and OCT field-of-views and concurrent acquisition of an en face SESLO image with each OCT cross-section. SESLO and OCT frames were acquired at 200 fps with 2560 x 2000 pix. (spectral x lateral). We show in vivo human ophthalmic imaging data using surgical microscope-integrated and slit-lamp imaging relays to demonstrate the utility of our SS-SESLO-OCT design. Our self-contained modular scan-head can be used for either intraoperative guidance or clinical diagnostics and reduces the complexity, cost, and maintenance required for clinical translation of these technologies. We believe concurrent multimodal SS-SESLO-OCT may benefit 1) intraoperative imaging by allowing for real-time surgical feedback, instrument tracking, and overlays of computationally extracted image-based surrogate biomarkers of disease, and 2) slit-lamp imaging by enabling aiming, image registration, and multi-field mosaicking.

  14. Real-time characterization of non-metallic inclusions by optical scanning and milling of steel samples

    Science.gov (United States)

    Herwig, Johannes; Buck, Christoph; Thurau, Matthias; Pauli, Josef; Luther, Wolfram

    2012-04-01

    The cleanliness of steel is described by the amount, size, composition, morphology, and distribution of nonmetallic inclusions (NMIs). These nonmetals are present because of natural physical-chemical effects, and because during continuous casting steel is accidentally contaminated with slag, refractories, and materials from casting moulds. NMIs influence the properties of steel. Therefore, in this paper, a combined milling and image processing system is proposed that mills and scans slices of steel samples to retrieve volumetric information about NMIs. The system is capable of scanning steel samples of 300 × 100 × 90mm3 in size at spatial resolutions of either 3, 5, 10, or 20μm and a volumetric resolution of 10μm within a few hours. After each milling operation the steel surface is captured by a moving large-area CCD image sensor. The optical system further consists of a distortion-free macro lens and diffuse coaxial lighting for brightfield illumination. Additional results using dome lighting are also presented. The interaction of an NMI with the milling cutter results in non-homogeneous NMI reflectances which carry information about the NMI's mass density and chemical compound. Although the steel surface is highly reflective, the milling cutter creates a periodic pattern of moldings which is accentuated by patterns of shadow and light. An adaptive wedge filter in the Fourier space dampens those artifacts. NMIs are binarized separately in every image by local thresholding. In order to reduce segmentation artifacts neighboring slices in the volumetric stack of images are filtered using morphological operators. A statistical analysis of the segmentation results estimates the macro cleanliness. Furthermore an interactive 3D visualization enables the exploration of NMIs and their distribution within the sample. Different viewing, filtering and sorting capabilities are implemented, like ordering NMIs with regard to their shape factor. It is expected that the study

  15. Investigation of nonlinear optical properties of various organic materials by the Z-scan method

    Science.gov (United States)

    Ganeev, R. A.; Boltaev, G. S.; Tugushev, R. I.; Usmanov, T.

    2012-06-01

    We have studied the nonlinear optical properties of various organic materials (vegetable oil, juice, wine, cognac, Coca-Cola and Fanta drinks, Nescafé coffee, tea, gasoline, clock oil, glycerol, and polyphenyl ether) that are used in everyday life. Their nonlinearities have been studied by the Z-scan method in the near-IR and visible spectral ranges. We have shown that the majority of samples possess a nonlinear absorption; however, some of the studied materials show a strong saturated absorption and nonlinear refraction. Red wine and glycerol proved to be the most interesting materials. For these samples, we have observed a change in the sign of the nonlinear absorption with increasing laser intensity, which was attributed to the competition between two-photon absorption and saturated absorption.

  16. Novel grid-based optical Braille conversion: from scanning to wording

    Science.gov (United States)

    Yoosefi Babadi, Majid; Jafari, Shahram

    2011-12-01

    Grid-based optical Braille conversion (GOBCO) is explained in this article. The grid-fitting technique involves processing scanned images taken from old hard-copy Braille manuscripts, recognising and converting them into English ASCII text documents inside a computer. The resulted words are verified using the relevant dictionary to provide the final output. The algorithms employed in this article can be easily modified to be implemented on other visual pattern recognition systems and text extraction applications. This technique has several advantages including: simplicity of the algorithm, high speed of execution, ability to help visually impaired persons and blind people to work with fax machines and the like, and the ability to help sighted people with no prior knowledge of Braille to understand hard-copy Braille manuscripts.

  17. Adaptive optical versus spherical aberration corrections for in vivo brain imaging.

    Science.gov (United States)

    Turcotte, Raphaël; Liang, Yajie; Ji, Na

    2017-08-01

    Adjusting the objective correction collar is a widely used approach to correct spherical aberrations (SA) in optical microscopy. In this work, we characterized and compared its performance with adaptive optics in the context of in vivo brain imaging with two-photon fluorescence microscopy. We found that the presence of sample tilt had a deleterious effect on the performance of SA-only correction. At large tilt angles, adjusting the correction collar even worsened image quality. In contrast, adaptive optical correction always recovered optimal imaging performance regardless of sample tilt. The extent of improvement with adaptive optics was dependent on object size, with smaller objects having larger relative gains in signal intensity and image sharpness. These observations translate into a superior performance of adaptive optics for structural and functional brain imaging applications in vivo, as we confirmed experimentally.

  18. Adaptive optics imaging of healthy and abnormal regions of retinal nerve fiber bundles of patients with glaucoma.

    Science.gov (United States)

    Chen, Monica F; Chui, Toco Y P; Alhadeff, Paula; Rosen, Richard B; Ritch, Robert; Dubra, Alfredo; Hood, Donald C

    2015-01-08

    To better understand the nature of glaucomatous damage of the macula, especially the structural changes seen between relatively healthy and clearly abnormal (AB) retinal regions, using an adaptive optics scanning light ophthalmoscope (AO-SLO). Adaptive optics SLO images and optical coherence tomography (OCT) vertical line scans were obtained on one eye of seven glaucoma patients, with relatively deep local arcuate defects on the 10-2 visual field test in one (six eyes) or both hemifields (one eye). Based on the OCT images, the retinal nerve fiber (RNF) layer was divided into two regions: (1) within normal limits (WNL), relative RNF layer thickness within mean control values ±2 SD; and (2) AB, relative thickness less than -2 SD value. As seen on AO-SLO, the pattern of AB RNF bundles near the border of the WNL and AB regions differed across eyes. There were normal-appearing bundles in the WNL region of all eyes and AB-appearing bundles near the border with the AB region. This region with AB bundles ranged in extent from a few bundles to the entire AB region in the case of one eye. All other eyes had a large AB region without bundles. However, in two of these eyes, a few bundles were seen within this region of otherwise missing bundles. The AO-SLO images revealed details of glaucomatous damage that are difficult, if not impossible, to see with current OCT technology. Adaptive optics SLO may prove useful in following progression in clinical trials, or in disease management, if AO-SLO becomes widely available and easy to use. Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.

  19. Optic nerve sheath diameter measurements by CT scan in ventriculoperitoneal shunt obstruction.

    Science.gov (United States)

    Zaidi, Syed Javed H; Yamamoto, Loren G

    2014-08-01

    The objective of the study was to determine differences in optic nerve sheath diameter (ONSD) measurements taken from computed tomography (CT) scans of patients with ventriculoperitoneal shunt (VPS) obstruction versus controls. Inpatients 0-15 years with confirmed VPS obstruction requiring neurosurgical intervention were identified using ICD9 codes. ONSDs, orbit, cranium, and foramen magnum sizes were measured on their pre-surgical CT. Controls included cases at times when their VPS was not obstructed and age and gender matched patients with a CT scan done in the emergency room for head trauma (normal CT findings). Paired T-tests were used for both case-control comparisons. In order to compare the optic nerve sheath size more accurately, the ONSD width was divided by the width of the orbit and by the foramen magnum (antero-posterior) length. Twenty patients were identified with 25 events of VPS obstruction. The right ONSD (RON) was chosen to study. RON/orbit width and RON/foramen magnum diameter for the VPS obstruction versus self-controls, were 0.22 and 0.22, compared to 0.19 and 0.18, respectively, for the non-obstructed self-controls (P = .044 and P = .008, respectively). The same measurements for the VPS obstruction versus age and gender matched controls were 0.22 and 0.21 for the VPS obstruction cases, respectively, compared to 0.17 and 0.16, respectively for the age and gender matched controls (P VPS obstruction. ONSD measurements by ultrasound could add to the evaluation for VPS obstruction.

  20. Investigation of the variability of anterior chamber scan protocol with Cirrus high definition optical coherence tomography.

    Science.gov (United States)

    Tun, Tin A; Tan, Shayne S; Atalay, Eray; Verma, Sushma; Nongpiur, Monisha E; Baskaran, Mani; Aung, Tin; Husain, Rahat

    2017-07-01

    The evaluation of anterior chamber scan of Cirrus optical coherence tomography for routine clinical use. To assess the variability of anterior chamber angle measurements. This was a cross-sectional study. Forty subjects aged 40-80 years were included. One randomly selected eye from 40 subjects was imaged with Cirrus optical coherence tomography (Carl Zeiss Meditec, Dublin, CA) by two different operators (expert vs. non-expert) with a 15-min interval for inter-observer and intra-observer variability of image acquisition. For image grading, the angle opening distance (AOD750) and the trabecular iris space area (TISA750) of nasal and temporal quadrants were measured with a customized algorithm (ImageJ, NIH, Bethesda, MD) by two different graders in a masked and random fashion. Bland Altman analysis and intraclass correlation coefficient (ICC) were calculated. ICC and limit of agreements (LOA). There were 15 (37.5%) eyes with closed angles. For inter-observer variability, the mean difference (95% LOA) of AOD750 for image acquisition and grading were -0.0039 mm (-0.0486, 0.0408) and 0.0011 mm (-0.0228, 0.025), respectively. The mean difference (95% LOA) of AOD750 for intra-observer variability for image acquisition and grading were 0.0013 mm (-0.0362, 0.0389) and -0.0013 mm (-0.0482, 0.0457), respectively. The ICCs were all ≥0.9. There was no significant difference in measurement variability between open and closed angles (P > 0.05). Anterior chamber scan had low inter-observer and intra-observer variability in quantitative evaluation that was not affected by the angle status or the experience of an operator. © 2017 Royal Australian and New Zealand College of Ophthalmologists.

  1. All-optical histology using two photon laser scanning microscopy and ablation with ultrashort pulses

    Science.gov (United States)

    Tsai, Philbert S.

    This dissertation discusses the use of ultrashort laser pulses to image and manipulate tissue for the purpose of three-dimensional histological reconstruction of extended brain structures. Two photon laser scanning microscopy (TPLSM) and ultrashort pulsed laser ablation are used to provide in situ three-dimensional imaging through thick preparations of fixed tissue. Surface regions of fixed tissue are first imaged using TPLSM. The imaged regions are then removed by ablation with amplified, ultrashort laser pulses, thereby exposing a previously underlying tissue region for imaging. This process of imaging and ablation proceeds iteratively until the desired tissue volume has been processed. First, the principles, design, and construction of a two photon laser scanning microscope are discussed, followed by a discussion of the physical mechanisms of tissue ablation with ultrashort laser pulses. The compatibility of tissue ablation using ultrashort pulses with subsequent histological analysis, particularly with fluorescent microscopy, is evaluated. Tissue ablation with ultrashort laser pulses is found to produce ablated tissue surfaces that are smooth to within a micrometer. Intrinsic fluorescence as well as immunoreactivity are found to be resilient to the ablation process. The all-optical histological technique is demonstrated on brain tissue from rats and mice, including tissue from embryonic mouse as early at E15. The ablation process is shown to preserve both macroscopic and microscopic structures within tissue. To facilitate the all-optical histological analysis of neuronal vasculature and its relative distribution to surrounding neuronal tissue, a fluorescent gel perfusion technique is developed that provides a temperature-stabilized fluorescent label of the neuronal vasculature. The use of immunohistochemistry to label specific cell populations throughout an 800 micrometer-thick tissue section is demonstrated. Additionally, the immersion of fixed tissue in high

  2. Scanning laser optical tomography resolves structural plasticity during regeneration in an insect brain.

    Directory of Open Access Journals (Sweden)

    René Eickhoff

    Full Text Available BACKGROUND: Optical Projection Tomography (OPT is a microscopic technique that generates three dimensional images from whole mount samples the size of which exceeds the maximum focal depth of confocal laser scanning microscopes. As an advancement of conventional emission-OPT, Scanning Laser Optical Tomography (SLOTy allows simultaneous detection of fluorescence and absorbance with high sensitivity. In the present study, we employ SLOTy in a paradigm of brain plasticity in an insect model system. METHODOLOGY: We visualize and quantify volumetric changes in sensory information procession centers in the adult locust, Locusta migratoria. Olfactory receptor neurons, which project from the antenna into the brain, are axotomized by crushing the antennal nerve or ablating the entire antenna. We follow the resulting degeneration and regeneration in the olfactory centers (antennal lobes and mushroom bodies by measuring their size in reconstructed SLOTy images with respect to the untreated control side. Within three weeks post treatment antennal lobes with ablated antennae lose as much as 60% of their initial volume. In contrast, antennal lobes with crushed antennal nerves initially shrink as well, but regain size back to normal within three weeks. The combined application of transmission-and fluorescence projections of Neurobiotin labeled axotomized fibers confirms that recovery of normal size is restored by regenerated afferents. Remarkably, SLOTy images reveal that degeneration of olfactory receptor axons has a trans-synaptic effect on second order brain centers and leads to size reduction of the mushroom body calyx. CONCLUSIONS: This study demonstrates that SLOTy is a suitable method for rapid screening of volumetric plasticity in insect brains and suggests its application also to vertebrate preparations.

  3. Multimodal ophthalmic imaging using spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography

    Science.gov (United States)

    El-Haddad, Mohamed T.; Malone, Joseph D.; Li, Jianwei D.; Bozic, Ivan; Arquitola, Amber M.; Joos, Karen M.; Patel, Shriji N.; Tao, Yuankai K.

    2017-08-01

    Ophthalmic surgery involves manipulation of delicate, layered tissue structures on milli- to micrometer scales. Traditional surgical microscopes provide an inherently two-dimensional view of the surgical field with limited depth perception which precludes accurate depth-resolved visualization of these tissue layers, and limits the development of novel surgical techniques. We demonstrate multimodal swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography (SS-SESLO-OCT) to address current limitations of image-guided ophthalmic microsurgery. SS-SESLO-OCT provides inherently co-registered en face and cross-sectional field-of-views (FOVs) at a line rate of 400 kHz and >2 GPix/s throughput. We show in vivo imaging of the anterior segment and retinal fundus of a healthy volunteer, and preliminary results of multi-volumetric mosaicking for ultrawide-field retinal imaging with 90° FOV. Additionally, a scan-head was rapid-prototyped with a modular architecture which enabled integration of SS-SESLO-OCT with traditional surgical microscope and slit-lamp imaging optics. Ex vivo surgical maneuvers were simulated in cadaveric porcine eyes. The system throughput enabled volumetric acquisition at 10 volumes-per-second (vps) and allowed visualization of surgical dynamics in corneal sweeps, compressions, and dissections, and retinal sweeps, compressions, and elevations. SESLO en face images enabled simple real-time co-registration with the surgical microscope FOV, and OCT cross-sections provided depth-resolved visualization of instrument-tissue interactions. Finally, we demonstrate novel augmented-reality integration with the surgical view using segmentation overlays to aid surgical guidance. SS-SESLO-OCT may benefit clinical diagnostics by enabling aiming, registration, and mosaicking; and intraoperative imaging by allowing for real-time surgical feedback, instrument tracking, and overlays of computationally extracted biomarkers of disease.

  4. Image quality of multiplanar reconstruction of pulmonary CT scans using adaptive statistical iterative reconstruction

    Science.gov (United States)

    Honda, O; Yanagawa, M; Inoue, A; Kikuyama, A; Yoshida, S; Sumikawa, H; Tobino, K; Koyama, M; Tomiyama, N

    2011-01-01

    Objective We investigated the image quality of multiplanar reconstruction (MPR) using adaptive statistical iterative reconstruction (ASIR). Methods Inflated and fixed lungs were scanned with a garnet detector CT in high-resolution mode (HR mode) or non-high-resolution (HR) mode, and MPR images were then reconstructed. Observers compared 15 MPR images of ASIR (40%) and ASIR (80%) with those of ASIR (0%), and assessed image quality using a visual five-point scale (1, definitely inferior; 5, definitely superior), with particular emphasis on normal pulmonary structures, artefacts, noise and overall image quality. Results The mean overall image quality scores in HR mode were 3.67 with ASIR (40%) and 4.97 with ASIR (80%). Those in non-HR mode were 3.27 with ASIR (40%) and 3.90 with ASIR (80%). The mean artefact scores in HR mode were 3.13 with ASIR (40%) and 3.63 with ASIR (80%), but those in non-HR mode were 2.87 with ASIR (40%) and 2.53 with ASIR (80%). The mean scores of the other parameters were greater than 3, whereas those in HR mode were higher than those in non-HR mode. There were significant differences between ASIR (40%) and ASIR (80%) in overall image quality (pASIR did not suppress the severe artefacts of contrast medium. Conclusion In general, MPR image quality with ASIR (80%) was superior to that with ASIR (40%). However, there was an increased incidence of artefacts by ASIR when CT images were obtained in non-HR mode. PMID:21081572

  5. Signal of microstrip scanning near-field optical microscope in far- and near-field zones.

    Science.gov (United States)

    Morozov, Yevhenii M; Lapchuk, Anatoliy S

    2016-05-01

    An analytical model of interference between an electromagnetic field of fundamental quasi-TM(EH)00-mode and an electromagnetic field of background radiation at the apex of a near-field probe based on an optical plasmon microstrip line (microstrip probe) has been proposed. The condition of the occurrence of electromagnetic energy reverse flux at the apex of the microstrip probe was obtained. It has been shown that the nature of the interference depends on the length of the probe. Numerical simulation of the sample scanning process was conducted in illumination-reflection and illumination-collection modes. Results of numerical simulation have shown that interference affects the scanning signal in both modes. However, in illumination-collection mode (pure near-field mode), the signal shape and its polarity are practically insensible to probe length change; only signal amplitude (contrast) is slightly changed. However, changing the probe length strongly affects the signal amplitude and shape in the illumination-reflection mode (the signal formed in the far-field zone). Thus, we can conclude that even small background radiation can significantly influence the signal in the far-field zone and has practically no influence on a pure near-field signal.

  6. Adaptive Q control for Tapping-mode Nano-scanning Using a Piezo-actuated Bimorph Probe

    CERN Document Server

    Gunev, Ihsan; Karaman, Sertac; Basdogan, Cagatay

    2012-01-01

    A new approach, called Adaptive Q-control, for tapping-mode Atomic Force Microscopy (AFM) is introduced and implemented on a home-made AFM set-up utilizing a Laser Doppler Vibrometer (LDV) and a piezo-actuated bimorph probe. In the standard Q-control, the effective Q-factor of the scanning probe is adjusted prior to the scanning depending on the application. However, there is a trade-off in setting the effective Q-factor of an AFM probe. The Q-factor is either increased to reduce the tapping forces or decreased to increase the maximum achievable scan speed. Realizing these two benefits simultaneously using the standard Q-control is not possible. In adaptive Q-control, the Q-factor of the probe is set to an initial value as in standard Q-control, but then modified on the fly during scanning when necessary to achieve this goal. In this paper, we present the basic theory behind the adaptive Q-control, the electronics enabling the on-line modification of the probe's effective Q-factor, and the results of the expe...

  7. EN FACE VERSUS 12-LINE RADIAL OPTICAL COHERENCE TOMOGRAPHY SCAN PATTERNS FOR DETECTION OF MACULAR FLUID IN NEOVASCULAR AGE-RELATED MACULAR DEGENERATION.

    Science.gov (United States)

    Adam, Murtaza K; Shahlaee, Abtin; Samara, Wasim A; Maguire, Joseph I; Ho, Allen C; Hsu, Jason

    2017-08-14

    To compare fluid detection of autosegmented en face to 12-line radial spectral domain optical coherence tomography scan patterns in neovascular age-related macular degeneration. Retrospective observational case series. Sixty-seven patients (94 eyes) with neovascular age-related macular degeneration underwent autosegmented en face optical coherence tomography (with associated 304-line raster scan) and 12-line radial scan patterns. Sensitivity and specificity of fluid detection for en face scan and 12-line radial scans were determined by combining radial and 304-line raster scans as a gold standard. Two hundred and fifty-eight en face and 12-line radial spectral domain optical coherence tomography scans were interpreted. Seventy-five scans (58.1%) had fluid, whereas 54 scans (41.9%) did not. En face scan pattern fluid detection sensitivity and specificity was 89.3% and 61.1%, respectively. Twelve-line radial scan pattern fluid detection sensitivity and specificity was 97.3% and 100%, respectively. The difference in fluid detection between scan patterns was statistically significant (P = 0.01). Decreased central macular thickness was associated with false-positive (P = 0.035) and false-negative (P = 0.01) fluid detection on en face scans. En face optical coherence tomography alone is not as sensitive or specific as the 12-line radial scan pattern in detecting fluid in neovascular age-related macular degeneration. En face scans should be corroborated with other optical coherence tomography protocols to guide clinical decision making.

  8. Retinal degeneration in progressive supranuclear palsy measured by optical coherence tomography and scanning laser polarimetry.

    Science.gov (United States)

    Stemplewitz, Birthe; Kromer, Robert; Vettorazzi, Eik; Hidding, Ute; Frings, Andreas; Buhmann, Carsten

    2017-07-13

    This cross-sectional study compared the retinal morphology between patients with progressive supranuclear palsy (PSP) and healthy controls. (The retinal nerve fiber layer (RNFL) around the optic disc and the retina in the macular area of 22 PSP patients and 151 controls were investigated by spectral domain optical coherence tomography (SD-OCT). Additionally, the RNFL and the nerve fiber index (NFI) were measured by scanning laser polarimetry (SLP). Results of RNFL measurements with SD-OCT and SLP were compared to assess diagnostic discriminatory power. Applying OCT, PSP patients showed a smaller RNFL thickness in the inferior nasal and inferior temporal areas. The macular volume and the thickness of the majority of macular sectors were reduced compared to controls. SLP data showed a thinner RNFL thickness and an increase in the NFI in PSP patients. Sensitivity and specificity to discriminate PSP patients from controls were higher applying SLP than SD-OCT. Retinal changes did not correlate with disease duration or severity in any OCT or SLP measurement. PSP seems to be associated with reduced thickness and volume of the macula and reduction of the RNFL, independent of disease duration or severity. Retinal imaging with SD-OCT and SLP might become an additional tool in PSP diagnosis.

  9. Systematic simulations of aerosol optical property retrieval uncertainty for scanning polarimeters

    Directory of Open Access Journals (Sweden)

    K. Knobelspiesse

    2011-09-01

    Full Text Available Scanning polarimeters, which utilize multi-angle, multispectral polarimetric observations from aircraft and orbit, represent the next generation of instruments capable of determining aerosol and cloud properties remotely. Retrieval of these properties from observations, however, are not straightforward. Iterative minimization techniques are often used to match radiative transfer simulations to the observations, where the aerosol and cloud parameters of the optimal model match are considered the best estimate of what is physically present in the scene. If the radiative transfer model perturbation sensitivity, expressed as a Jacobian matrix, can be assessed at the solution, then the observation uncertainty can be projected into the domain of the retrieved parameters. These parameter uncertainties provide are an extremely useful means to assess retrieval success. Another aspect of our iterative minimization techniques is the need for a reasonable initial estimate of optical properties. This estimate is provided by matching observations to a Lookup Table (LUT of pre-computed radiative transfer scenes. This LUT spans a wide range of aerosol and cloud optical properties, and also includes numerical estimates of the Jacobian matrix at each element in the LUT. Using the Jacobians, we can estimate the retrieval uncertainty for all elements of the LUT, and therefore build a table of expected uncertainty. This paper presents how this approach is used in a systematic manner, and how it can be used to test retrieval capability for various combinations of polarized, multi-angle and multispectral observations.

  10. Scanning laser polarimetry, but not optical coherence tomography predicts permanent visual field loss in acute nonarteritic anterior ischemic optic neuropathy.

    Science.gov (United States)

    Kupersmith, Mark J; Anderson, Susan; Durbin, Mary; Kardon, Randy

    2013-08-15

    Scanning laser polarimetry (SLP) reveals abnormal retardance of birefringence in locations of the edematous peripapillary retinal nerve fiber layer (RNFL), which appear thickened by optical coherence tomography (OCT), in nonarteritic anterior ischemic optic neuropathy (NAION). We hypothesize initial sector SLP RNFL abnormalities will correlate with long-term regional visual field loss due to ischemic injury. We prospectively performed automated perimetry, SLP, and high definition OCT (HD-OCT) of the RNFL in 25 eyes with acute NAION. We grouped visual field threshold and RNFL values into Garway-Heath inferior/superior disc sectors and corresponding superior/inferior field regions. We compared sector SLP RNFL thickness with corresponding visual field values at presentation and at >3 months. At presentation, 12 eyes had superior sector SLP reduction, 11 of which had inferior field loss. Six eyes, all with superior field loss, had inferior sector SLP reduction. No eyes had reduced OCT-derived RNFL acutely. Eyes with abnormal field regions had corresponding SLP sectors thinner (P = 0.003) than for sectors with normal field regions. During the acute phase, the SLP-derived sector correlated with presentation (r = 0.59, P = 0.02) and with >3-month after presentation (r = 0.44, P = 0.02) corresponding superior and inferior field thresholds. Abnormal RNFL birefringence occurs in sectors corresponding to regional visual field loss during acute NAION when OCT-derived RNFL shows thickening. Since the visual field deficits show no significant recovery, SLP can be an early marker for axonal injury, which may be used to assess recovery potential at RNFL locations with respect to new treatments for acute NAION.

  11. Dynamic aberration correction for conformal optics using model-based wavefront sensorless adaptive optics

    Science.gov (United States)

    Han, Xinli; Dong, Bing; Li, Yan; Wang, Rui; Hu, Bin

    2016-10-01

    For missiles and airplanes with high Mach number, traditional spherical or flat window can cause a lot of air drag. Conformal window that follow the general contour of surrounding surface can substantially decrease air drag and extend operational range. However, the local shape of conformal window changes across the Field Of Regard (FOR), leading to time-varying FOR-dependent wavefront aberration and degraded image. So the correction of dynamic aberration is necessary. In this paper, model-based Wavefront Sensorless Adaptive Optics (WSAO) algorithm is investigated both by simulation and experiment for central-obscured pupil. The algorithm is proved to be effective and the correction accuracy of using DM modes is higher than Lukosz modes. For dynamic aberration in our system, the SR can be better than 0.8 when the change of looking angle is less than 2° after t seconds which is the time delay of the control system.

  12. Atmospheric refractivity effects on mid-infrared ELT adaptive optics

    Science.gov (United States)

    Kendrew, Sarah; Jolissaint, Laurent; Mathar, Richard J.; Stuik, Remko; Hippler, Stefan; Brandl, Bernhard

    2008-07-01

    We discuss the effect of atmospheric dispersion on the performance of a mid-infrared adaptive optics assisted instrument on an extremely large telescope (ELT). Dispersion and atmospheric chromaticity is generally considered to be negligible in this wavelength regime. It is shown here, however, that with the much-reduced diffraction limit size on an ELT and the need for diffraction-limited performance, refractivity phenomena should be carefully considered in the design and operation of such an instrument. We include an overview of the theory of refractivity, and the influence of infrared resonances caused by the presence of water vapour and other constituents in the atmosphere. 'Traditional' atmospheric dispersion is likely to cause a loss of Strehl only at the shortest wavelengths (L-band). A more likely source of error is the difference in wavelengths at which the wavefront is sensed and corrected, leading to pointing offsets between wavefront sensor and science instrument that evolve with time over a long exposure. Infrared radiation is also subject to additional turbulence caused by the presence of water vapour in the atmosphere not seen by visible wavefront sensors, whose effect is poorly understood. We make use of information obtained at radio wavelengths to make a first-order estimate of its effect on the performance of a mid-IR ground-based instrument. The calculations in this paper are performed using parameters from two different sites, one 'standard good site' and one 'high and dry site' to illustrate the importance of the choice of site for an ELT.

  13. Enhancing Stellar Spectroscopy with Extreme Adaptive Optics and Photonics

    Science.gov (United States)

    Jovanovic, N.; Schwab, C.; Cvetojevic, N.; Guyon, O.; Martinache, F.

    2016-12-01

    Extreme adaptive optics (AO) systems are now in operation across the globe. These systems, capable of high order wavefront correction, deliver Strehl ratios of ˜ 90 % in the near-infrared. Originally intended for the direct imaging of exoplanets, these systems are often equipped with advanced coronagraphs that suppress the on-axis-star, interferometers to calibrate wavefront errors, and low order wavefront sensors to stabilize any tip/tilt residuals to a degree never seen before. Such systems are well positioned to facilitate the detailed spectroscopic characterization of faint substellar companions at small angular separations from the host star. Additionally, the increased light concentration of the point-spread function and the unprecedented stability create opportunities in other fields of astronomy as well, including spectroscopy. With such Strehl ratios, efficient injection into single-mode fibers (SMFs) or photonic lanterns becomes possible. With diffraction-limited components feeding the instrument, calibrating a spectrograph’s line profile becomes considerably easier, as modal noise or imperfect scrambling of the fiber output are no longer an issue. It also opens up the possibility of exploiting photonic technologies for their advanced functionalities, inherent replicability, and small, lightweight footprint to design and build future instrumentation. In this work, we outline how extreme AO systems will enable advanced photonic and diffraction-limited technologies to be exploited in spectrograph design and the impact it will have on spectroscopy. We illustrate that the precision of an instrument based on these technologies, with light injected from an efficient SMF feed would be entirely limited by the spectral content and stellar noise alone on cool stars and would be capable of achieving a radial velocity precision of several m/s; the level required for detecting an exo-Earth in the habitable zone of a nearby M-dwarf.

  14. Probing other solar systems with current and future adaptive optics

    Energy Technology Data Exchange (ETDEWEB)

    Macintosh, B; Marois, C; Phillion, D; Poyneer, L; Graham, J; Zuckerman, B; Gavel, D; Veran, J; Wilhelmsen-Evans, J; Mellis, C

    2008-09-08

    Over the past decade, the study of extrasolar planets through indirect techniques--primarily Doppler measurements--has revolutionized our understanding of other solar systems. The next major step in this field will be the direct detection and characterization, via imaging and spectroscopy, of the planets themselves. To achieve this, we must separate the light from the faint planet from the extensive glare of its parent star. We pursued this goal using the current generation of adaptive optics (AO) systems on large ground-based telescopes, using infrared imaging to search for the thermal emission from young planets and developing image processing techniques to distinguish planets from telescope-induced artifacts. Our new Angular Differential Imaging (ADI) technique, which uses the sidereal rotation of the Earth and telescope, is now standard for ground-based high-contrast imaging. Although no young planets were found in our surveys, we placed the strongest limits yet on giant planets in wide orbits (>30 AU) around young stars and characterized planetary companion candidates. The imaging of planetary companions on solar-system-like scales (5-30 AU) will require a new generation of advanced AO systems that are an order of magnitude more powerful than the LLNL-built Keck AO system. We worked to develop and test the key technologies needed for these systems, including a spatially-filtered wavefront sensor, efficient and accurate wavefront reconstruction algorithms, and precision AO wavefront control at the sub-nm level. LLNL has now been selected by the Gemini Observatory to lead the construction of the Gemini Planet Imager, a $24M instrument that will be the most advanced AO system in the world.

  15. Electrostatic polymer-based microdeformable mirror for adaptive optics

    Science.gov (United States)

    Zamkotsian, Frederic; Conedera, Veronique; Granier, Hugues; Liotard, Arnaud; Lanzoni, Patrick; Salvagnac, Ludovic; Fabre, Norbert; Camon, Henri

    2007-02-01

    Future adaptive optics (AO) systems require deformable mirrors with very challenging parameters, up to 250 000 actuators and inter-actuator spacing around 500 μm. MOEMS-based devices are promising for the development of a complete generation of new deformable mirrors. Our micro-deformable mirror (MDM) is based on an array of electrostatic actuators with attachments to a continuous mirror on top. The originality of our approach lies in the elaboration of layers made of polymer materials. Mirror layers and active actuators have been demonstrated. Based on the design of this actuator and our polymer process, realization of a complete polymer-MDM has been done using two process flows: the first involves exclusively polymer materials while the second uses SU8 polymer for structural layers and SiO II and sol-gel for sacrificial layers. The latest shows a better capability in order to produce completely released structures. The electrostatic force provides a non-linear actuation, while AO systems are based on linear matrices operations. Then, we have developed a dedicated 14-bit electronics in order to "linearize" the actuation, using a calibration and a sixth-order polynomial fitting strategy. The response is nearly perfect over our 3×3 MDM prototype with a standard deviation of 3.5 nm; the influence function of the central actuator has been measured. First evaluation on the cross non-linarities has also been studied on OKO mirror and a simple look-up table is sufficient for determining the location of each actuator whatever the locations of the neighbor actuators. Electrostatic MDM are particularly well suited for open-loop AO applications.

  16. Using Adaptive Optics Follow-up to Characterize Microlensing Exoplanets

    Science.gov (United States)

    Henderson, Calen; Beichman, Charles; Shvartzvald, Yossi

    2018-01-01

    The mass and distance of a microlens are degenerate, thus requiring at least two relations to yield a unique solution. Measuring the finite-source effect from the light curve helps provide one mass-distance relation for the lens system. Currently, the primary avenue for establishing a second relation and thus uniquely solving for the mass and distance of the lens is by measuring the microlens parallax. One specific implementation is the satellite parallax technique, which involves taking observations simultaneously from two locations separated by a significant fraction of an AU, and which has been employed by Spitzer and K2's Campaign 9, transforming this methodology from a cottage industry to a booming economy. However, the majority of microlensing exoplanets to be discovered in the coming decades, up to and including the detections predicted for WFIRST, will not have a measurement of the satellite parallax, requiring another avenue for converting microlensing observables into physical parameters. Enter the lens flux characterization technique, through which a microlensing target is observed with a high-resolution facility, facilitating a constraint on the flux from the lens system. This yields a third mass-distance relation for the lens and can be combined with that from the detection of finite-source effects and/or the microlens parallax to determine the mass of the lens system as well as its distance from Earth. I will highlight recent programs using NIRC2 on Keck that are designed to make lens flux measurements for a myriad of exoplanetary lenses, including: (A) systems with high blend flux, which adaptive optics is perfectly suited to resolve; (B) systems with high relative lens-source proper motion; (C) free-floating planet candidates; and (D) bound exoplanets.

  17. Adaptive Optics for Satellite Imaging and Space Debris Ranging

    Science.gov (United States)

    Bennet, F.; D'Orgeville, C.; Price, I.; Rigaut, F.; Ritchie, I.; Smith, C.

    Earth's space environment is becoming crowded and at risk of a Kessler syndrome, and will require careful management for the future. Modern low noise high speed detectors allow for wavefront sensing and adaptive optics (AO) in extreme circumstances such as imaging small orbiting bodies in Low Earth Orbit (LEO). The Research School of Astronomy and Astrophysics (RSAA) at the Australian National University have been developing AO systems for telescopes between 1 and 2.5m diameter to image and range orbiting satellites and space debris. Strehl ratios in excess of 30% can be achieved for targets in LEO with an AO loop running at 2kHz, allowing the resolution of small features (system developed at RSAA consists of a high speed EMCCD Shack-Hartmann wavefront sensor, a deformable mirror (DM), and realtime computer (RTC), and an imaging camera. The system works best as a laser guide star system but will also function as a natural guide star AO system, with the target itself being the guide star. In both circumstances tip-tilt is provided by the target on the imaging camera. The fast tip-tilt modes are not corrected optically, and are instead removed by taking images at a moderate speed (>30Hz) and using a shift and add algorithm. This algorithm can also incorporate lucky imaging to further improve the final image quality. A similar AO system for space debris ranging is also in development in collaboration with Electro Optic Systems (EOS) and the Space Environment Management Cooperative Research Centre (SERC), at the Mount Stromlo Observatory in Canberra, Australia. The system is designed for an AO corrected upward propagated 1064nm pulsed laser beam, from which time of flight information is used to precisely range the target. A 1.8m telescope is used for both propagation and collection of laser light. A laser guide star, Shack-Hartmann wavefront sensor, and DM are used for high order correction, and tip-tilt correction provided by reflected sunlight from the target. The

  18. High resolution in situ magneto-optic Kerr effect and scanning tunneling microscopy setup with all optical components in UHV.

    Science.gov (United States)

    Lehnert, A; Buluschek, P; Weiss, N; Giesecke, J; Treier, M; Rusponi, S; Brune, H

    2009-02-01

    A surface magneto-optic Kerr effect (MOKE) setup fully integrated in an ultrahigh vacuum chamber is presented. The system has been designed to combine in situ MOKE and scanning tunneling microscopy. Magnetic fields up to 0.3 T can be applied at any angle in the transverse plane allowing the study of in-plane and out-of-plane magnetization. The setup performance is demonstrated for a continuous film of 0.9 monolayers (ML) Co/Rh(111) with in-plane easy axis and for a superlattice of nanometric double layer Co islands on Au(11,12,12) with out-of-plane easy axis. For Co/Au(11,12,12) we demonstrate that the magnetic anisotropy energies deduced from thermally induced magnetization reversal and from applying a torque onto the magnetization by turning the field are the same. For the presented setup we establish a coverage detection limit of 0.5 ML for transverse and 0.1 ML for polar MOKE. For island superlattices with the density of Co/Au(11,12,12), the latter limit corresponds to islands composed of about 50 atoms. The detection limit can be further reduced when optimizing the MOKE setup for either one of the two Kerr configurations.

  19. Performance analysis of an adaptive optics system for free-space optics communication through atmospheric turbulence.

    Science.gov (United States)

    Wang, Yukun; Xu, Huanyu; Li, Dayu; Wang, Rui; Jin, Chengbin; Yin, Xianghui; Gao, Shijie; Mu, Quanquan; Xuan, Li; Cao, Zhaoliang

    2018-01-18

    The performance of free-space optics communication (FSOC) is greatly degraded by atmospheric turbulence. Adaptive optics (AO) is an effective method for attenuating the influence. In this paper, the influence of the spatial and temporal characteristics of turbulence on the performance of AO in a FSOC system is investigated. Based on the Greenwood frequency (GF) and the ratio of receiver aperture diameter to atmospheric coherent length (D/r 0 ), the relationship between FSOC performance (CE) and AO parameters (corrected Zernike modes number and bandwidth) is derived for the first time. Then, simulations and experiments are conducted to analyze the influence of AO parameters on FSOC performance under different GF and D/r 0 . The simulation and experimental results show that, for common turbulence conditions, the number of corrected Zernike modes can be fixed at 35 and the bandwidth of the AO system should be larger than the GF. Measurements of the bit error rate (BER) for moderate turbulence conditions (D/r 0  = 10, f G  = 60 Hz) show that when the bandwidth is two times that of GF, the average BER is decreased by two orders of magnitude compared with f G /f 3dB  = 1. These results and conclusions can provide important guidance in the design of an AO system for FSOC.

  20. Visualization of micro-capillaries using optical coherence tomography angiography with and without adaptive optics.

    Science.gov (United States)

    Salas, Matthias; Augustin, Marco; Ginner, Laurin; Kumar, Abhishek; Baumann, Bernhard; Leitgeb, Rainer; Drexler, Wolfgang; Prager, Sonja; Hafner, Julia; Schmidt-Erfurth, Ursula; Pircher, Michael

    2017-01-01

    The purpose of this work is to investigate the benefits of adaptive optics (AO) technology for optical coherence tomography angiography (OCTA). OCTA has shown great potential in non-invasively enhancing the contrast of vessels and small capillaries. Especially the capability of the technique to visualize capillaries with a lateral extension that is below the transverse resolution of the system opens unique opportunities in diagnosing retinal vascular diseases. However, there are some limitations of this technology such as shadowing and projection artifacts caused by overlying vasculature or the inability to determine the true extension of a vessel. Thus, the evaluation of the vascular structure and density based on OCTA alone can be misleading. In this paper we compare the performance of AO-OCT, AO-OCTA and OCTA for imaging retinal vasculature. The improved transverse resolution and the reduced depth of focus of AO-OCT and AO-OCTA greatly reduce shadowing artifacts allowing for a better differentiation and segmentation of different vasculature layers of the inner retina. The comparison is done on images recorded in healthy volunteers and in diabetic patients with distinct pathologies of the retinal microvasculature.

  1. Adaptive optics-assisted optical coherence tomography for imaging of patients with age related macular degeneration

    Science.gov (United States)

    Sudo, Kenta; Cense, Barry

    2013-03-01

    We developed an optical coherence tomography (OCT) prototype with a sample arm that uses a 3.4 mm beam, which is considerably larger than the 1.2 to 1.5 mm beam that is used in commercialized OCT systems. The system is equipped with adaptive optics (AO), and to distinguish it from traditional AO-OCT systems with a larger 6 mm beam we have coined this concept AO-assisted OCT. Compared to commercialized OCT systems, the 3.4 mm aperture combined with AO improves light collection efficiency and imaging lateral resolution. In this paper, the performance of the AOa-OCT system was compared to a standard OCT system and demonstrated for imaging of age-related macular degeneration (AMD). Measurements were performed on the retinas of three human volunteers with healthy eyes and on one eye of a patient diagnosed with AMD. The AO-assisted OCT system imaged retinal structures of healthy human eyes and a patient eye affected by AMD with higher lateral resolution and a 9° by 9° field of view. This combination of a large isoplanatic patch and high lateral resolution can be expected to fill a gap between standard OCT with a 1.2 mm beam and conventional AO-OCT with a 6 mm beam and a 1.5° by 1.5° isoplanatic patch.

  2. IMAGING WITH MULTIMODAL ADAPTIVE-OPTICS OPTICAL COHERENCE TOMOGRAPHY IN MULTIPLE EVANESCENT WHITE DOT SYNDROME: THE STRUCTURE AND FUNCTIONAL RELATIONSHIP.

    Science.gov (United States)

    Labriola, Leanne T; Legarreta, Andrew D; Legarreta, John E; Nadler, Zach; Gallagher, Denise; Hammer, Daniel X; Ferguson, R Daniel; Iftimia, Nicusor; Wollstein, Gadi; Schuman, Joel S

    2016-01-01

    To elucidate the location of pathological changes in multiple evanescent white dot syndrome (MEWDS) with the use of multimodal adaptive optics (AO) imaging. A 5-year observational case study of a 24-year-old female with recurrent MEWDS. Full examination included history, Snellen chart visual acuity, pupil assessment, intraocular pressures, slit lamp evaluation, dilated fundoscopic exam, imaging with Fourier-domain optical coherence tomography (FD-OCT), blue-light fundus autofluorescence (FAF), fundus photography, fluorescein angiography, and adaptive-optics optical coherence tomography. Three distinct acute episodes of MEWDS occurred during the period of follow-up. Fourier-domain optical coherence tomography and adaptive-optics imaging showed disturbance in the photoreceptor outer segments (PR OS) in the posterior pole with each flare. The degree of disturbance at the photoreceptor level corresponded to size and extent of the visual field changes. All findings were transient with delineation of the photoreceptor recovery from the outer edges of the lesion inward. Hyperautofluorescence was seen during acute flares. Increase in choroidal thickness did occur with each active flare but resolved. Although changes in the choroid and RPE can be observed in MEWDS, Fourier-domain optical coherence tomography, and multimodal adaptive optics imaging localized the visually significant changes seen in this disease at the level of the photoreceptors. These transient retinal changes specifically occur at the level of the inner segment ellipsoid and OS/RPE line. En face optical coherence tomography imaging provides a detailed, yet noninvasive method for following the convalescence of MEWDS and provides insight into the structural and functional relationship of this transient inflammatory retinal disease.

  3. Hot dust in the active nucleus of NGC 7469 probed by adaptive optics observations

    Science.gov (United States)

    Marco, O.; Alloin, D.

    1998-08-01

    The use of adaptive optics has allowed to reach in the infrared an angular resolution around 0.35'', allowing to study the temperature, mass and distribution of the dust component within the 400 pc radius central region of \

  4. Diagnostic capability of scanning laser polarimetry with and without enhanced corneal compensation and optical coherence tomography.

    Science.gov (United States)

    Benítez-del-Castillo, Javier; Martinez, Antonio; Regi, Teresa

    2011-01-01

    To compare the abilities of the current commercially available versions of scanning laser polarimetry (SLP) and optical coherence tomography (OCT), SLP-variable corneal compensation (VCC), SLP-enhanced corneal compensation (ECC), and high-definition (HD) OCT, in discriminating between healthy eyes and those with early-to-moderate glaucomatous visual field loss. Healthy volunteers and patients with glaucoma who met the eligibility criteria were consecutively enrolled in this prospective, cross-sectional, observational study. Subjects underwent complete eye examination, automated perimetry, SLP-ECC, SLP-VCC, and HD-OCT. Scanning laser polarimetry parameters were recalculated in 90-degree segments (quadrants) in the calculation circle to be compared. Areas under the receiver operating characteristic curve (AUROCs) were calculated for every parameter in order to compare the ability of each imaging modality to differentiate between normal and glaucomatous eyes. Fifty-five normal volunteers (mean age 59.1 years) and 33 patients with glaucoma (mean age 63.8 years) were enrolled. Average visual field mean deviation was -6.69 dB (95% confidence interval -8.07 to -5.31) in the glaucoma group. The largest AUROCs were associated with nerve fiber indicator (0.880 and 0.888) for the SLP-VCC and SLP-ECC, respectively, and with the average thickness in the HD-OCT (0.897). The best performing indices for the SLP-VCC, SLP-ECC, and HD OCT gave similar AUROCs, showing moderate diagnostic accuracy in patients with early to moderate glaucoma. Further studies are needed to evaluate the ability of these technologies to discriminate between normal and glaucomatous eyes.

  5. Predictive Factors for Visual Field Conversion: Comparison of Scanning Laser Polarimetry and Optical Coherence Tomography.

    Science.gov (United States)

    Diekmann, Theresa; Schrems-Hoesl, Laura M; Mardin, Christian Y; Laemmer, Robert; Horn, Folkert K; E Kruse, Friedrich; Schrems, Wolfgang A

    2017-11-30

    The purpose of this study was to compare the ability of scanning laser polarimetry (SLP) and spectral-domain optical coherence tomography (SD-OCT) to predict future visual field conversion of subjects with ocular hypertension and early glaucoma. All patients were recruited from the Erlangen glaucoma registry and examined using standard automated perimetry, 24-hour intraocular pressure profile, and optic disc photography. Peripapillary retinal nerve fiber layer thickness (RNFL) measurements were obtained by SLP (GDx-VCC) and SD-OCT (Spectralis OCT). Positive and negative predictive values (PPV, NPV) were calculated for morphologic parameters of SLP and SD-OCT. Kaplan-Meier survival curves were plotted and log-rank tests were performed to compare the survival distributions. Contingency tables and Venn-diagrams were calculated to compare the predictive ability. The study included 207 patients-75 with ocular hypertension, 85 with early glaucoma, and 47 controls. Median follow-up was 4.5 years. A total of 29 patients (14.0%) developed visual field conversion during follow-up. SLP temporal-inferior RNFL [0.667; 95% confidence interval (CI), 0.281-0.935] and SD-OCT temporal-inferior RNFL (0.571; 95% CI, 0.317-0.802) achieved the highest PPV; nerve fiber indicator (0.923; 95% CI, 0.876-0.957) and SD-OCT mean (0.898; 95% CI, 0.847-0.937) achieved the highest NPV of all investigated parameters. The Kaplan-Meier curves confirmed significantly higher survival for subjects within normal limits of measurements of both devices (P<0.001). Venn diagrams tested with McNemar test statistics showed no significant difference for PPV (P=0.219) or NPV (P=0.678). Both GDx-VCC and SD-OCT demonstrate comparable results in predicting future visual field conversion if taking typical scans for GDx-VCC. In addition, the likelihood ratios suggest that GDx-VCC's nerve fiber indicator<30 may be the most useful parameter to confirm future nonconversion. (http://www.ClinicalTrials.gov number, NTC

  6. Uncrowding R 136 from VLT/SPHERE extreme adaptive optics

    Science.gov (United States)

    Khorrami, Z.; Vakili, F.; Lanz, T.; Langlois, M.; Lagadec, E.; Meyer, M. R.; Robbe-Dubois, S.; Abe, L.; Avenhaus, H.; Beuzit, J. L.; Gratton, R.; Mouillet, D.; Origné, A.; Petit, C.; Ramos, J.

    2017-06-01

    This paper presents the sharpest near-IR images of the massive cluster R 136 to date, based on the extreme adaptive optics of the SPHERE focal instrument implemented on the ESO Very Large Telescope and operated in its IRDIS imaging mode.The crowded stellar population in the core of the R 136 starburst compact cluster remains still to be characterized in terms of individual luminosities, age, mass and multiplicity. SPHERE/VLT and its high contrast imaging possibilities open new windows to make progress on these questions.Stacking-up a few hundreds of short exposures in J and Ks spectral bands over a field of view (FoV) of 10.9″ × 12.3″ centered on the R 136a1 stellar component, enabled us to carry a refined photometric analysis of the core of R 136. We detected 1110 and 1059 sources in J and Ks images respectively with 818 common sources. Thanks to better angular resolution and dynamic range, we found that more than 62.6% (16.5%) of the stars, detected both in J and Ks data, have neighbours closer than 0.2'' (0.1''). The closest stars are resolved down to the full width at half maximum (FWHM) of the point spread function (PSF) measured by Starfinder. Among resolved and/or detected sources R 136a1 and R 136c have optical companions and R 136a3 is resolved as two stars (PSF fitting) separated by 59 ± 2 mas. This new companion of R 136a3 presents a correlation coefficient of 86% in J and 75% in Ks. The new set of detected sources were used to re-assess the age and extinction of R 136 based on 54 spectroscopically stars that have been recently studied with HST slit-spectroscopy (Crowther et al. 2016, MNRAS, 458, 624) of the core of this cluster. Over 90% of these 54 sources identified visual companions (closer than 0.2''). We found the most probable age and extinction for these sources are Myr, AJ = (0.45 ± 0.5) mag and AK = (0.2 ± 0.5) mag within the photometric and spectroscopic error-bars. Additionally, using PARSEC evolutionary isochrones and tracks, we

  7. Direct characterization of ultraviolet-light-induced refractive index structures by scanning near-field optical microscopy

    DEFF Research Database (Denmark)

    Svalgaard, Mikael; Madsen, S.; Hvam, Jørn Märcher

    1998-01-01

    We have applied a reflection scanning near-field optical microscope to directly probe ultraviolet (UV)-light-induced refractive index structures in planar glass samples. This technique permits direct comparison between topography and refractive index changes (10(-5)-10(-3)) with submicrometer...... lateral resolution, The proposed method yields detailed information about the topography and index profiles of UV-written waveguides....

  8. Scanning near-field optical microscopy on rough surfaces: applications in chemistry, biology, and medicine

    Directory of Open Access Journals (Sweden)

    2006-01-01

    Full Text Available Shear-force apertureless scanning near-field optical microscopy (SNOM with very sharp uncoated tapered waveguides relies on the unexpected enhancement of reflection in the shear-force gap. It is the technique for obtaining chemical (materials contrast in the optical image of “real world” surfaces that are rough and very rough without topographical artifacts, and it is by far less complicated than other SNOM techniques that can only be used for very flat surfaces. The experimental use of the new photophysical effect is described. The applications of the new technique are manifold. Important mechanistic questions in solid-state chemistry (oxidation, diazotization, photodimerization, surface hydration, hydrolysis are answered with respect to simultaneous AFM (atomic force microscopy and detailed crystal packing. Prehistoric petrified bacteria and concomitant pyrite inclusions are also investigated with local RAMAN SNOM. Polymer beads and unstained biological objects (rabbit heart, shrimp eye allow for nanoscopic analysis of cell organelles. Similarly, human teeth and a cancerous tissue are analyzed. Bladder cancer tissue is clearly differentiated from healthy tissue without staining and this opens a new highly promising diagnostic tool for precancer diagnosis. Industrial applications are demonstrated at the corrosion behavior of dental alloys (withdrawal of a widely used alloy, harmless substitutes, improvement of paper glazing, behavior of blood bags upon storage, quality assessment of metal particle preparations for surface enhanced RAMAN spectroscopy, and determination of diffusion coefficient and light fastness in textile fiber dyeing. The latter applications include fluorescence SNOM. Local fluorescence SNOM is also used in the study of partly aggregating dye nanoparticles within resin/varnish preparations. Unexpected new insights are obtained in all of the various fields that cannot be obtained by other techniques.

  9. Comparison of Optical Coherence Tomography and Scanning Laser Polarimetry Measurements in Patients with Multiple Sclerosis

    Science.gov (United States)

    Quelly, Amanda; Cheng, Han; Laron, Michal; Schiffman, Jade S.; Tang, Rosa A.

    2010-01-01

    Purpose To compare optical coherence tomography (OCT) and scanning laser polarimetry (GDx) measurements of the retinal nerve fiber layer (RNFL) in multiple sclerosis (MS) patients with and without optic neuritis (ON). Methods OCT and GDx were performed on 68 MS patients. Qualifying eyes were divided into two groups: 51 eyes with an ON history ≥ 6 months prior (ON eyes), and 65 eyes with no history of ON (non-ON eyes). Several GDx and OCT parameters and criteria were used to define an eye as abnormal, for example, GDx nerve fiber indicator (NFI) above 20 or 30, OCT average RNFL thickness and GDx temporal-superior-nasal-inferior-temporal average (TSNIT) below 5% or 1% of the instruments’ normative database. Agreement between OCT and GDx parameters was reported as percent of observed agreement, along with the AC1 statistic. Linear regression analyses were used to examine the relationship between OCT average RNFL thickness and GDx NFI and TSNIT. Results All OCT and GDx measurements showed significantly more RNFL damage in ON than in non-ON eyes. Agreement between OCT and GDx parameters ranged from 69–90% (AC1 0.37–0.81) in ON eyes, and 52–91% (AC1 = 0.21–0.90) in non-ON eyes. Best agreement was observed between OCT average RNFL thickness (P 30) in ON eyes (90%, AC1 = 0.81), and between OCT average RNFL thickness (P < 0.01) and GDx TSNIT average (P < 0.01) in non-ON eyes (91%, AC1 = 0.90). In ON eyes, the OCT average RNFL thickness showed good linear correlation with NFI (R2 = 0.69, P < 0.0001) and TSNIT (R2 = 0.55, P < 0.0001). Conclusions OCT and GDx show good agreement and can be useful in detecting RNFL loss in MS/ON eyes. PMID:20495500

  10. Tuning of successively scanned two monolithic Vernier-tuned lasers and selective data sampling in optical comb swept source optical coherence tomography.

    Science.gov (United States)

    Choi, Dong-Hak; Yoshimura, Reiko; Ohbayashi, Kohji

    2013-01-01

    Monolithic Vernier tuned super-structure grating distributed Bragg reflector (SSG-DBR) lasers are expected to become one of the most promising sources for swept source optical coherence tomography (SS-OCT) with a long coherence length, reduced sensitivity roll-off, and potential capability for a very fast A-scan rate. However, previous implementations of the lasers suffer from four main problems: 1) frequencies deviate from the targeted values when scanned, 2) large amounts of noise appear associated with abrupt changes in injection currents, 3) optically aliased noise appears due to a long coherence length, and 4) the narrow wavelength coverage of a single chip limits resolution. We have developed a method of dynamical frequency tuning, a method of selective data sampling to eliminate current switching noise, an interferometer to reduce aliased noise, and an excess-noise-free connection of two serially scanned lasers to enhance resolution to solve these problems. An optical frequency comb SS-OCT system was achieved with a sensitivity of 124 dB and a dynamic range of 55-72 dB that depended on the depth at an A-scan rate of 3.1 kHz with a resolution of 15 μm by discretely scanning two SSG-DBR lasers, i.e., L-band (1.560-1.599 μm) and UL-band (1.598-1.640 μm). A few OCT images with excellent image penetration depth were obtained.

  11. Wavefront Reconstruction and Mirror Surface Optimizationfor Adaptive Optics

    Science.gov (United States)

    2014-06-01

    Optical Society of America, vol. 70, no. 8, pp. 998 –1006, Aug. 1980. [Online]. Available: http://www.opticsinfobase.org/abstract.cfm?URI=josa-70-8- 998 [26...optics systems using wavelets,” IEEE Journal of Selected Topics in Signal Processing, vol. 2, no. 5, pp. 781–792, Oct. 2008. [Online]. Available: http

  12. The experiment to detect equivalent optical path difference in independent double aperture interference light path based on step scanning method

    Science.gov (United States)

    Wang, Chaoyan; Chen, Xin-yang; Zheng, Lixin; Ding, Yuanyuan

    2014-11-01

    Fringe test is the method which can detect the relative optical path difference in optical synthetic aperture telescope array. To get to the interference fringes, the two beams of light in the meeting point must be within the coherence length. Step scanning method is within its coherence length, selecting a specific step, changing one-way's optical path of both by changing position of micro displacement actuator. At the same time, every fringe pattern can be recorded. The process of fringe patterns is from appearing to clear to disappearing. Firstly, a particular pixel is selected. Then, we keep tract of the intensity of every picture in the same position. From the intensity change, the best position of relative optical path difference can be made sure. The best position of relative optical path difference is also the position of the clearest fringe. The wavelength of the infrared source is 1290nm and the bandwidth is 63.6nm. In this experiment, the coherence length of infrared source is detected by cube reflection experiment. The coherence length is 30μm by data collection and data processing, and that result of 30μm is less different from the 26μm of theoretical calculated. In order to further test the relative optical path of optical synthetic aperture using step scanning method, the infrared source is placed into optical route of optical synthesis aperture telescope double aperture. The precision position of actuator can be obtained when the fringe is the clearest. By the experiment, we found that the actuating step affects the degree of precision of equivalent optical path. The smaller step size, the more accurate position. But the smaller the step length, means that more steps within the coherence length measurement and the longer time.

  13. Near-field scanning magneto-optical spectroscopy of Wigner molecules

    Energy Technology Data Exchange (ETDEWEB)

    Mintairov, A. M., E-mail: amintair@nd.edu; Rouvimov, S. [University of Notre Dame, Notre Dame, IN 46556 (United States); Ioffe Physical-Technical Institute of the Russian Academy of Sciences, Saint Petersburg, 194021 (Russian Federation); Kapaldo, J. [University of Notre Dame, Notre Dame, IN 46556 (United States); Merz, J. L.; Kalyygniy, N.; Mintairov, S. A.; Nekrasov, S.; Saly, R.; Vlasov, A. S. [Ioffe Physical-Technical Institute of the Russian Academy of Sciences, Saint Petersburg, 194021 (Russian Federation); Blundell, S. [SPSMS, UMR-E CEA/UJF-Grenoble 1, INAC, Grenoble, FR-38054 (France)

    2016-06-17

    We study the emission spectra of single self-organized InP/GaInP QDs (size 100-220 nm) using high-spatial-resolution, low-temperature (5 K) near-field scanning optical microscope (NSOM) operating at magnetic field strength B=0-10 T. The dots contain up to twenty electrons and represent natural Wigner molecules (WM). We observed vibronic-type shake-up structure in single electron QDs manifesting formation of two electron (2e) WM in photo-excited state. We found that relative intensities of the shake-up components described well by vibronic Frank-Condon factors giving for dots having parabolic confinement energy ħω{sub 0}=1.2-4 meV molecule bond lengths 40-140 nm. We used measurements of magnetic-field-induced shifts to distinguish emission of 2e-WM and singly charged exciton (trion). We also observed magnetic-field-induced molecular-droplet transition for two electron dot, emitting through doubly charge exciton (tetron) at zero magnetic field.

  14. Inducing superconductivity at a nanoscale: photodoping with a near-field scanning optical microscope.

    Science.gov (United States)

    Decca, R S; Drew, H D; Maiorov, B; Guimpel, J; Osquiguil, E J

    1999-01-01

    The local modification of an insulating GdBa2Cu3O6.5 thin film, made superconducting by illumination with a near-field scanning optical microscope (NSOM), is reported. A 100-nm aperture NSOM probe acts as a sub-wavelength light source of wavelength lambda(exc) = 480-650 nm, locally generating photocarriers in an otherwise insulating GdBa2-Cu3O6.5 thin film. Of the photogenerated electron-hole pairs, electrons are trapped in the crystallographic lattice, defining an electrostatic confining potential to enable the holes to move. Reflectance measurements at lambda = 1.55 microm at room temperature show that photocarriers can be induced and constrained to move on a approximately 200 nm scale for all investigated lambda(exc). Photogenerated wires present a superconducting critical temperature Tc= 12 K with a critical current density Jc = 10(4) A cm(-2). Exploiting the flexibility provided by photodoping through a NSOM probe, a junction was written by photodoping a wire with a narrow (approximately 50 nm) under-illuminated gap. The strong magnetic field modulation of the critical current provides a clear signature of the existence of a Josephson effect in the junction.

  15. Feasibility of fiber optic displacement sensor scanning system for imaging of dental cavity

    Science.gov (United States)

    Rahman, Husna Abdul; Che Ani, Adi Izhar; Harun, Sulaiman Wadi; Yasin, Moh.; Apsari, Retna; Ahmad, Harith

    2012-07-01

    The purpose of this study is to investigate the potential of intensity modulated fiber optic displacement sensor scanning system for the imaging of dental cavity. Here, we discuss our preliminary results in the imaging of cavities on various teeth surfaces, as well as measurement of the diameter of the cavities which are represented by drilled holes on the teeth surfaces. Based on the analysis of displacement measurement, the sensitivities and linear range for the molar, canine, hybrid composite resin, and acrylic surfaces are obtained at 0.09667 mV/mm and 0.45 mm 0.775 mV/mm and 0.4 mm 0.5109 mV/mm and 0.5 mm and 0.25 mV/mm and 0.5 mm, respectively, with a good linearity of more than 99%. The results also show a clear distinction between the cavity and surrounding tooth region. The stability, simplicity of design, and low cost of fabrication make it suitable for restorative dentistry.

  16. Line-scan Raman microscopy complements optical coherence tomography for tumor boundary detection

    Science.gov (United States)

    Sudheendran, Narendran; Qi, Ji; Young, Eric D.; Lazar, Alexander J.; Lev, Dina C.; Pollock, Raphael E.; Larin, Kirill V.; Shih, Wei-Chuan

    2014-10-01

    Current technique for tumor resection requires biopsy of the tumor region and histological confirmation before the surgeon can be certain that the entire tumor has been resected. This confirmation process is time consuming both for the surgeon and the patient and also requires sacrifice of healthy tissue, motivating the development of novel technologies which can enable real-time detection of tumor-healthy tissue boundary for faster and more efficient surgeries. In this study, the potential of combining structural information from optical coherence tomography (OCT) and molecular information from line-scan Raman microscopy (LSRM) for such an application is presented. The results show a clear presence of boundary between myxoid liposarcoma and normal fat which is easily identifiable both from structural and molecular information. In cases where structural images are indistinguishable, for example, in normal fat and well differentiated liposarcoma (WDLS) or gastrointestinal sarcoma tumor (GIST) and myxoma, distinct molecular spectra have been obtained. The results suggest LSRM can effectively complement OCT to tumor boundary demarcation with high specificity.

  17. Development of beam monitoring system for proton pencil beam scanning using fiber-optic radiation sensor

    Science.gov (United States)

    Son, Jaeman; Koo, Jihye; Moon, Sunyoung; Yoon, Myonggeun; Jeong, Jonghwi; Kim, Sun-Young; Lim, Youngkyung; Lee, Se Byeong; Shin, Dongho; Kim, Meyoung; Kim, Dongwook

    2017-10-01

    We aimed to develop a beam monitoring system based on a fiber-optic radiation sensor (FORS), which can be used in real time in a beam control room, to monitor a beam in proton therapy, where patients are treated using a pencil beam scanning (PBS) mode, by measuring the beam spot width (BSW) and beam spot position (BSP) of the PBS. We developed two-dimensional detector arrays to monitor the PBS beam in the beam control room. We measured the BSW for five energies of the PBS beam and compared the measurements with those of Lynx and EBT3 film. In order to confirm the BSP, we compared the BSP values of the PBS calculated from radiation treatment planning (RTP), to five BSP values measured using FORS at 224.2 MeV. When comparing BSW values obtained using developed monitoring system to the measurements obtained using commercial EBT3 film, the average difference in BSW value of the PBS beam was 0.1 ± 0.1 mm. In the comparison of BSW values with the measurements obtained using Lynx, the average difference was 0.2 ± 0.1 mm. When comparing BSP measurements to the values calculated from RTP, the average difference was 0.4 ± 0.2 mm. The study results confirmed that the developed FORS-based beam monitoring system can monitor a PBS beam in real time in a beam control room, where proton beam is controlled for the patient.

  18. Phase imaging and detection in pseudo-heterodyne scattering scanning near-field optical microscopy measurements.

    Science.gov (United States)

    Moreno, Camilo; Alda, Javier; Kinzel, Edward; Boreman, Glenn

    2017-02-01

    When considering the pseudo-heterodyne mode for detection of the modulus and phase of the near field from scattering scanning near-field optical microscopy (s-SNOM) measurements, processing only the modulus of the signal may produce an undesired constraint in the accessible values of the phase of the near field. A two-dimensional analysis of the signal provided by the data acquisition system makes it possible to obtain phase maps over the whole [0, 2π) range. This requires post-processing of the data to select the best coordinate system in which to represent the data along the direction of maximum variance. The analysis also provides a quantitative parameter describing how much of the total variance is included within the component selected for calculation of the modulus and phase of the near field. The dependence of the pseudo-heterodyne phase on the mean position of the reference mirror is analyzed, and the evolution of the global phase is extracted from the s-SNOM data. The results obtained from this technique compared well with the expected maps of the near-field phase obtained from simulations.

  19. Optical scanning system for light-absorption measurement of deep biological tissue.

    Science.gov (United States)

    Funane, Tsukasa; Atsumori, Hirokazu; Kiguchi, Masashi; Tanikawa, Yukari; Okada, Eiji

    2011-09-01

    A noncontact near-infrared scanning system for multi-distance absorption measurement of deep biological tissue was developed. An 808-nm laser, whose focal point on the surface of biological tissue is controlled by a galvano scanner, is used as a light source. A phosphor is placed at a detection focal point on the tissue surface. The light that propagates through tissue and exits from the tissue surface beneath the phosphor excites the phosphor. The fluorescence emitted from the phosphor is detected by an avalanche photodiode. The system is used to measure 20 points on tissue surface at which source-detector (S-D) distances are 7-45 mm (with 2-mm intervals). Neither the light source nor the detector contacts the tissue surface. The system was validated by using it to measure the absorption change of an absorber (which is embedded in a deep layer of a tissue-simulating phantom) while the surface-layer thickness of the phantom was changed from 1 to 12 mm. It was demonstrated that both the relative absorption change of the absorber and the absolute thickness of the surface layer can be estimated from the measured optical-density change (ΔOD) and the dependence of ΔOD on S-D distance, respectively. © 2011 American Institute of Physics

  20. Rigorous numerical modeling of scattering-type scanning near-field optical microscopy and spectroscopy

    Science.gov (United States)

    Chen, Xinzhong; Lo, Chiu Fan Bowen; Zheng, William; Hu, Hai; Dai, Qing; Liu, Mengkun

    2017-11-01

    Over the last decade, scattering-type scanning near-field optical microscopy and spectroscopy have been widely used in nano-photonics and material research due to their fine spatial resolution and broad spectral range. A number of simplified analytical models have been proposed to quantitatively understand the tip-scattered near-field signal. However, a rigorous interpretation of the experimental results is still lacking at this stage. Numerical modelings, on the other hand, are mostly done by simulating the local electric field slightly above the sample surface, which only qualitatively represents the near-field signal rendered by the tip-sample interaction. In this work, we performed a more comprehensive numerical simulation which is based on realistic experimental parameters and signal extraction procedures. By directly comparing to the experiments as well as other simulation efforts, our methods offer a more accurate quantitative description of the near-field signal, paving the way for future studies of complex systems at the nanoscale.

  1. LGSD/NGSD: high speed optical CMOS imagers for E-ELT adaptive optics

    Science.gov (United States)

    Downing, Mark; Kolb, Johann; Balard, Philippe; Dierickx, Bart; Defernez, Arnaud; Feautrier, Philippe; Finger, Gert; Fryer, Martin; Gach, Jean-Luc; Guillaume, Christian; Hubin, Norbert; Jerram, Paul; Jorden, Paul; Meyer, Manfred; Payne, Andrew; Pike, Andrew; Reyes, Javier; Simpson, Robert; Stadler, Eric; Stent, Jeremy; Swift, Nick

    2014-07-01

    The success of the next generation of instruments for ELT class telescopes will depend upon improving the image quality by exploiting sophisticated Adaptive Optics (AO) systems. One of the critical components of the AO systems for the E-ELT has been identified as the optical Laser/Natural Guide Star WFS detector. The combination of large format, 1760×1680 pixels to finely sample the wavefront and the spot elongation of laser guide stars, fast frame rate of 700 frames per second (fps), low read noise ( 90%) makes the development of this device extremely challenging. Design studies concluded that a highly integrated Backside Illuminated CMOS Imager built on High Resistivity silicon as the most likely technology to succeed. Two generations of the CMOS Imager are being developed: a) the already designed and manufactured NGSD (Natural Guide Star Detector), a quarter-sized pioneering device of 880×840 pixels capable of meeting first light needs of the E-ELT; b) the LGSD (Laser Guide Star Detector), the larger full size device. The detailed design is presented including the approach of using massive parallelism (70,400 ADCs) to achieve the low read noise at high pixel rates of ~3 Gpixel/s and the 88 channel LVDS 220Mbps serial interface to get the data off-chip. To enable read noise closer to the goal of 1e- to be achieved, a split wafer run has allowed the NGSD to be manufactured in the more speculative, but much lower read noise, Ultra Low Threshold Transistors in the unit cell. The NGSD has come out of production, it has been thinned to 12μm, backside processed and packaged in a custom 370pin Ceramic PGA (Pin Grid Array). First results of tests performed both at e2v and ESO are presented.

  2. Development of a scanning nearfield optical microscope for low-temperature investigations of semiconductor nanostructures

    Energy Technology Data Exchange (ETDEWEB)

    Hodeck, Kai Friedrich

    2009-02-19

    In the present work the electronic structure of MOCVD-grown InGaAs/GaAs and InAs/GaAs quantum dots which are characterized by a particularly low ground state transition energy, was investigated using Scanning Nearfield Optical Microscopy (SNOM). The pivotal question of the presented investigations is, which influence the interaction of the confined carriers has on the energy states of the biexcitons and the multiexcitons in a quantum dot. Therefore, photoluminescence spectra of single quantum dots were investigated under varying excitation intensity at different temperatures between 5 K and 300 K. The construction of a novel scanning nearfield microscope especially for low temperatures allowed the investigation of single quantum dots. Due to significant improvements of the positioning technology and the shear-force distance control between the sample and the nearfield probe a stable scanning of the quantum dot samples at 5 K could be demonstrated, showing a lateral optical resolution of 200 nm. This way, in the photoluminescence spectroscopy of single quantum dots the thermal linewidth broadening of the detected light was reduced down to a value of less than 1 meV, which allowed the identification of the transitions of biexcitons and multiexcitons. On the basis of the performed measurements, for the InGaAs/GaAs quantum dots a biexciton state was identified, with variable binding energies of 2-7 meV. Furthermore, a positively charged trion state with a binding energy of 11 meV was observed, showing high emission intensity, which can be assigned to the sample doping. Accordingly, for the positively charged biexciton state a binding energy of 11 meV can be announced. For the investigated InAs/GaAs quantum dots a biexciton state with binding energies of 3-4 meV was found. Some of the investigated InAs/GaAs quantum dots showed the formation of positively charged states, in particular of a trion state with a binding energy of 3 meV, and of the positively charged

  3. Breaking RAD: an evaluation of the utility of restriction site-associated DNA sequencing for genome scans of adaptation.

    Science.gov (United States)

    Lowry, David B; Hoban, Sean; Kelley, Joanna L; Lotterhos, Katie E; Reed, Laura K; Antolin, Michael F; Storfer, Andrew

    2017-03-01

    Understanding how and why populations evolve is of fundamental importance to molecular ecology. Restriction site-associated DNA sequencing (RADseq), a popular reduced representation method, has ushered in a new era of genome-scale research for assessing population structure, hybridization, demographic history, phylogeography and migration. RADseq has also been widely used to conduct genome scans to detect loci involved in adaptive divergence among natural populations. Here, we examine the capacity of those RADseq-based genome scan studies to detect loci involved in local adaptation. To understand what proportion of the genome is missed by RADseq studies, we developed a simple model using different numbers of RAD-tags, genome sizes and extents of linkage disequilibrium (length of haplotype blocks). Under the best-case modelling scenario, we found that RADseq using six- or eight-base pair cutting restriction enzymes would fail to sample many regions of the genome, especially for species with short linkage disequilibrium. We then surveyed recent studies that have used RADseq for genome scans and found that the median density of markers across these studies was 4.08 RAD-tag markers per megabase (one marker per 245 kb). The length of linkage disequilibrium for many species is one to three orders of magnitude less than density of the typical recent RADseq study. Thus, we conclude that genome scans based on RADseq data alone, while useful for studies of neutral genetic variation and genetic population structure, will likely miss many loci under selection in studies of local adaptation. © 2016 John Wiley & Sons Ltd.

  4. Fluorescent correlation spectroscopy measurements with adaptive optics in the intercellular space of spheroids.

    Science.gov (United States)

    Leroux, Charles-Edouard; Monnier, Sylvain; Wang, Irène; Cappello, Giovanni; Delon, Antoine

    2014-10-01

    In this study we demonstrate the use of adaptive optics to correct the biasing effects of optical aberrations when measuring the dynamics of molecules diffusing between cells in multicellular spheroids. Our results indicate that, on average, adaptive optics leads to a reduction of the 3D size of the point spread function that is statistically significant in terms of measured number of molecules and diffusion time. The sensorless approach, which uses the molecular brightness as optimization metric, is validated in a complex, highly heterogeneous, biological environment. This work paves the way towards the design of accurate diffusion measurements of molecules in thick biological specimens.

  5. An Optical OFDM Modem with Adaptive Volterra Equalizer

    Science.gov (United States)

    Tawade, Laxman; Pinjarkar, Umesh; Awade, Kavita; Bapu Aboobacker, Abida; Gosavi, Manisha; Bhatlawande, Yogeshwari

    2015-03-01

    It addresses orthogonal frequency division multiplexing (OFDM) transmission over optical links with high spectral efficiency, i.e. by using high-order quadrature amplitude modulation (QAM) schemes as a mapping method prior to the OFDM multicarrier representation. Here we address especially coherent optical OFDM modem in long distance which is affected by nonlinear distortion caused by fiber nonlinearity. Fiber nonlinearity is a majo performance-limiting factor in advanced optical communication systems. We proposed a nonlinear electrical equalization scheme based on the Volterra model. To compare with other popular linear compensation technique such as the least mean square (LMS), simulation results are presented to demonstrate the capability of a Volterra model based electrical equalizer used in a coherent optical orthogonal frequency division multiplexing system. It is shown that the Volterra model based equalizer can significantly reduce nonlinear distortion.

  6. Advancement of an Interferometric Flow Velocity Measurement Technique by Adaptive Optics

    Science.gov (United States)

    Büttner, Lars; Leithold, Christoph; Czarske, Jürgen

    2014-01-01

    Flow measurements often take place under difficult conditions. Optical flow measurement techniques are affected by variations of the refractive index, caused e.g., by temperature, concentration, or pressure gradients. This will give rise to an increased measurement uncertainty or cause the measurement to fail. To overcome these limitations, we propose the employment of adaptive optics. In this contribution we present interferometric flow velocity measurements through a fluctuating air-water interface by the use of adaptive optics. Using the adaptive optics, the rate of valid measurement signals can be improved from 28% to 83%. The results are promising to enable measurements in difficult environments affected by refractive index variations which were not accessible so far.

  7. High-Resolution Adaptive Optics Test-Bed for Vision Science

    Energy Technology Data Exchange (ETDEWEB)

    Wilks, S C; Thomspon, C A; Olivier, S S; Bauman, B J; Barnes, T; Werner, J S

    2001-09-27

    We discuss the design and implementation of a low-cost, high-resolution adaptive optics test-bed for vision research. It is well known that high-order aberrations in the human eye reduce optical resolution and limit visual acuity. However, the effects of aberration-free eyesight on vision are only now beginning to be studied using adaptive optics to sense and correct the aberrations in the eye. We are developing a high-resolution adaptive optics system for this purpose using a Hamamatsu Parallel Aligned Nematic Liquid Crystal Spatial Light Modulator. Phase-wrapping is used to extend the effective stroke of the device, and the wavefront sensing and wavefront correction are done at different wavelengths. Issues associated with these techniques will be discussed.

  8. Oxidation of hydrogen-passivated silicon surfaces by scanning near-field optical lithography using uncoated and aluminum-coated fiber probes

    DEFF Research Database (Denmark)

    Madsen, Steen; Bozhevolnyi, Sergey I.; Birkelund, Karen

    1997-01-01

    Optically induced oxidation of hydrogen-passivated silicon surfaces using a scanning near-field optical microscope was achieved with both uncoated and aluminum-coated fiber probes. Line scans on amorphous silicon using uncoated fiber probes display a three-peak profile after etching in potassium...

  9. Adaptive optics fundus images of cone photoreceptors in the macula of patients with retinitis pigmentosa.

    Science.gov (United States)

    Tojo, Naoki; Nakamura, Tomoko; Fuchizawa, Chiharu; Oiwake, Toshihiko; Hayashi, Atsushi

    2013-01-01

    The purpose of this study was to examine cone photoreceptors in the macula of patients with retinitis pigmentosa using an adaptive optics fundus camera and to investigate any correlations between cone photoreceptor density and findings on optical coherence tomography and fundus autofluorescence. We examined two patients with typical retinitis pigmentosa who underwent ophthalmological examination, including measurement of visual acuity, and gathering of electroretinographic, optical coherence tomographic, fundus autofluorescent, and adaptive optics fundus images. The cone photoreceptors in the adaptive optics images of the two patients with retinitis pigmentosa and five healthy subjects were analyzed. An abnormal parafoveal ring of high-density fundus autofluorescence was observed in the macula in both patients. The border of the ring corresponded to the border of the external limiting membrane and the inner segment and outer segment line in the optical coherence tomographic images. Cone photoreceptors at the abnormal parafoveal ring were blurred and decreased in the adaptive optics images. The blurred area corresponded to the abnormal parafoveal ring in the fundus autofluorescence images. Cone densities were low at the blurred areas and at the nasal and temporal retina along a line from the fovea compared with those of healthy controls. The results for cone spacing and Voronoi domains in the macula corresponded with those for the cone densities. Cone densities were heavily decreased in the macula, especially at the parafoveal ring on high-density fundus autofluorescence in both patients with retinitis pigmentosa. Adaptive optics images enabled us to observe in vivo changes in the cone photoreceptors of patients with retinitis pigmentosa, which corresponded to changes in the optical coherence tomographic and fundus autofluorescence images.

  10. Hardware adaptation layer for MPEG video recording on a helical scan-based digital data recorder

    Science.gov (United States)

    de Ridder, Ad C.; Kindt, S.; Frimout, Emmanuel D.; Biemond, Jan; Lagendijk, Reginald L.

    1996-03-01

    The forthcoming introduction of helical scan digital data tape recorders with high access bandwidth and large capacity will facilitate the recording and retrieval of a wide variety of multimedia information from different sources, such as computer data and digital audio and video. For the compression of digital audio and video, the MPEG standard has internationally been accepted. Although helical scan tape recorders can store and playback MPEG compressed signals transparently they are not well suited for carrying out special playback modes, in particular fast forward and fast reverse. Only random portions of a original MPEG bitstream are recovered on fast playback. Unfortunately these shreds of information cannot be interpreted by a standard MPEG decoder, due to loss of synchronization and missing reference pictures. In the EC-sponsored RACE project DART (Digital Data Recorder Terminal) the possibilities for recording and fast playback of MPEG video on a helical scan recorder have been investigated. In the approach we present in this paper, we assume that not transcoding is carried out on the incoming bitstream at recording time, nor that any additional information is recorded. To use the shreds of information for the reconstruction of interpretable pictures, a bitstream validator has been developed to achieve conformance to the MPEG-2 syntax during fast playback. The concept has been validated by realizing hardware demonstrators that connect to a prototype helical scan digital data tape recorder.

  11. Structure-function relationships using spectral-domain optical coherence tomography: comparison with scanning laser polarimetry.

    Science.gov (United States)

    Aptel, Florent; Sayous, Romain; Fortoul, Vincent; Beccat, Sylvain; Denis, Philippe

    2010-12-01

    To evaluate and compare the regional relationships between visual field sensitivity and retinal nerve fiber layer (RNFL) thickness as measured by spectral-domain optical coherence tomography (OCT) and scanning laser polarimetry. Prospective cross-sectional study. One hundred and twenty eyes of 120 patients (40 with healthy eyes, 40 with suspected glaucoma, and 40 with glaucoma) were tested on Cirrus-OCT, GDx VCC, and standard automated perimetry. Raw data on RNFL thickness were extracted for 256 peripapillary sectors of 1.40625 degrees each for the OCT measurement ellipse and 64 peripapillary sectors of 5.625 degrees each for the GDx VCC measurement ellipse. Correlations between peripapillary RNFL thickness in 6 sectors and visual field sensitivity in the 6 corresponding areas were evaluated using linear and logarithmic regression analysis. Receiver operating curve areas were calculated for each instrument. With spectral-domain OCT, the correlations (r(2)) between RNFL thickness and visual field sensitivity ranged from 0.082 (nasal RNFL and corresponding visual field area, linear regression) to 0.726 (supratemporal RNFL and corresponding visual field area, logarithmic regression). By comparison, with GDx-VCC, the correlations ranged from 0.062 (temporal RNFL and corresponding visual field area, linear regression) to 0.362 (supratemporal RNFL and corresponding visual field area, logarithmic regression). In pairwise comparisons, these structure-function correlations were generally stronger with spectral-domain OCT than with GDx VCC and with logarithmic regression than with linear regression. The largest areas under the receiver operating curve were seen for OCT superior thickness (0.963 ± 0.022; P polarimetry, and was better expressed logarithmically than linearly. Measurements with these 2 instruments should not be considered to be interchangeable. Copyright © 2010 Elsevier Inc. All rights reserved.

  12. Scanning laser polarimetry and optical coherence tomography for detection of retinal nerve fiber layer defects.

    Science.gov (United States)

    Oh, Jong-Hyun; Kim, Yong Yeon

    2009-09-01

    To compare the ability of scanning laser polarimetry with variable corneal compensation (GDx-VCC) and Stratus optical coherence tomography (OCT) to detect photographic retinal nerve fiber layer (RNFL) defects. This retrospective cross-sectional study included 45 eyes of 45 consecutive glaucoma patients with RNFL defects in red-free fundus photographs. The superior and inferior temporal quadrants in each eye were included for data analysis separately. The location and presence of RNFL defects seen in red-free fundus photographs were compared with those seen in GDx-VCC deviation maps and OCT RNFL analysis maps for each quadrant. Of the 90 quadrants (45 eyes), 31 (34%) had no apparent RNFL defects, 29 (32%) had focal RNFL defects, and 30 (33%) had diffuse RNFL defects in red-free fundus photographs. The highest agreement between GDx-VCC and red-free photography was 73% when we defined GDx-VCC RNFL defects as a cluster of three or more color-coded squares (p<5%) along the traveling line of the retinal nerve fiber in the GDx-VCC deviation map (kappa value, 0.388; 95% confidence interval (CI), 0.195 to 0.582). The highest agreement between OCT and red-free photography was 85% (kappa value, 0.666; 95% CI, 0.506 to 0.825) when a value of 5% outside the normal limit for the OCT analysis map was used as a cut-off value for OCT RNFL defects. According to the kappa values, the agreement between GDx-VCC deviation maps and red-free photography was poor, whereas the agreement between OCT analysis maps and red-free photography was good.

  13. Genome-Wide Scans for Candidate Genes Involved in the Aquatic Adaptation of Dolphins

    Science.gov (United States)

    Liu, He-Qun; Irwin, David M.; Shen, Yong-Yi; Zhang, Ya-Ping

    2013-01-01

    Since their divergence from the terrestrial artiodactyls, cetaceans have fully adapted to an aquatic lifestyle, which represents one of the most dramatic transformations in mammalian evolutionary history. Numerous morphological and physiological characters of cetaceans have been acquired in response to this drastic habitat transition, such as thickened blubber, echolocation, and ability to hold their breath for a long period of time. However, knowledge about the molecular basis underlying these adaptations is still limited. The sequence of the genome of Tursiops truncates provides an opportunity for a comparative genomic analyses to examine the molecular adaptation of this species. Here, we constructed 11,838 high-quality orthologous gene alignments culled from the dolphin and four other terrestrial mammalian genomes and screened for positive selection occurring in the dolphin lineage. In total, 368 (3.1%) of the genes were identified as having undergone positive selection by the branch-site model. Functional characterization of these genes showed that they are significantly enriched in the categories of lipid transport and localization, ATPase activity, sense perception of sound, and muscle contraction, areas that are potentially related to cetacean adaptations. In contrast, we did not find a similar pattern in the cow, a closely related species. We resequenced some of the positively selected sites (PSSs), within the positively selected genes, and showed that most of our identified PSSs (50/52) could be replicated. The results from this study should have important implications for our understanding of cetacean evolution and their adaptations to the aquatic environment. PMID:23246795

  14. Time-resolved ultraviolet near-field scanning optical microscope for characterizing photoluminescence lifetime of light-emitting devices.

    Science.gov (United States)

    Park, Kyoung-Duck; Jeong, Hyun; Kim, Yong Hwan; Yim, Sang-Youp; Lee, Hong Seok; Suh, Eun-Kyung; Jeong, Mun Seok

    2013-03-01

    We developed a instrument consisting of an ultraviolet (UV) near-field scanning optical microscope (NSOM) combined with time-correlated single photon counting, which allows efficient observation of temporal dynamics of near-field photoluminescence (PL) down to the sub-wavelength scale. The developed time-resolved UV NSOM system showed a spatial resolution of 110 nm and a temporal resolution of 130 ps in the optical signal. The proposed microscope system was successfully demonstrated by characterizing the near-field PL lifetime of InGaN/GaN multiple quantum wells.

  15. Axial-Stereo 3-D Optical Metrology for Inner Profile of Pipes Using a Scanning Laser Endoscope.

    Science.gov (United States)

    Gong, Yuanzheng; Johnston, Richard S; Melville, C David; Seibel, Eric J

    As the rapid progress in the development of optoelectronic components and computational power, 3D optical metrology becomes more and more popular in manufacturing and quality control due to its flexibility and high speed. However, most of the optical metrology methods are limited to external surfaces. This paper proposed a new approach to measure tiny internal 3D surfaces with a scanning fiber endoscope and axial-stereo vision algorithm. A dense, accurate point cloud of internally machined threads was generated to compare with its corresponding X-ray 3D data as ground truth, and the quantification was analyzed by Iterative Closest Points algorithm.

  16. Nonlinear optical properties of natural laccaic acid dye studied using Z-scan technique

    CSIR Research Space (South Africa)

    Zongo, S

    2015-08-01

    Full Text Available We have investigated the nonlinear optical properties, including the optical limiting behaviour for five different concentrations of laccaic acid dye in solution and a thin film obtained through doping in poly (methyl methacrylate) (PMMA) polymer...

  17. Adaptive Optics for EUV Lithography : Phase Retrieval for Wavefront Metrology

    NARCIS (Netherlands)

    Polo, A.

    2014-01-01

    In the semiconductor industry, optical lithography is presently the most widespread technology used to print a geometrical pattern on a semiconductor wafer. Because of the plans imposed by the International Technology Roadmap for Semiconductors (ITRS) for more powerful and smaller chips, new

  18. Broad band nonlinear optical absorption measurements of the laser dye IR26 using white light continuum Z-scan

    Science.gov (United States)

    Dey, Soumyodeep; Bongu, Sudhakara Reddy; Bisht, Prem Ballabh

    2017-03-01

    We study the nonlinear optical response of a standard dye IR26 using the Z-scan technique, but with the white light continuum. The continuum source of wavelength from 450 nm to 1650 nm has been generated from the photonic crystal fiber on pumping with 772 nm of Ti:Sapphire oscillator. The use of broadband incident pulse enables us to probe saturable absorption (SA) and reverse saturable absorption (RSA) over the large spectral range with a single Z-scan measurement. The system shows SA in the resonant region while it turns to RSA in the non-resonant regions. The low saturation intensity of the dye can be explained based on the simultaneous excitation from ground states to various higher energy levels with the help of composite energy level diagram. The cumulative effects of excited state absorption and thermal induced nonlinear optical effects are responsible for the observed RSA.

  19. Adaptive image content-based exposure control for scanning applications in radiography

    NARCIS (Netherlands)

    Schulerud, H.; Thielemann, J.; Kirkhus, T.; Kaspersen, K.; Østby, J.M.; Metaxas, M.G.; Royle, G.J.; Griffiths, J.; Cook, E.; Esbrand, C.; Pani, S.; Venanzi, C.; van der Stelt, P.F.; Li, G.; Turchetta, R.; Fant, A.; Theodoridis, S.; Georgiou, H.; Hall, G.; Noy, M.; Jones, J.; Leaver, J.; Triantis, F.; Asimidis, A.; Manthos, N.; Longo, R.; Bergamaschi, A.; Speller, R.D.

    2007-01-01

    I-ImaS (Intelligent Imaging Sensors) is a European project which has designed and developed a new adaptive X-ray imaging system using on-line exposure control, to create locally optimized images. The I-ImaS system allows for real-time image analysis during acquisition, thus enabling real-time

  20. Whole-Genome Scans Provide Evidence of Adaptive Evolution in Malawian Plasmodium falciparum Isolates

    DEFF Research Database (Denmark)

    Ocholla, Harold; Preston, Mark D; Mipando, Mwapatsa

    2014-01-01

    BACKGROUND:  Selection by host immunity and antimalarial drugs has driven extensive adaptive evolution in Plasmodium falciparum and continues to produce ever-changing landscapes of genetic variation. METHODS:  We performed whole-genome sequencing of 69 P. falciparum isolates from Malawi and used...

  1. Investigation of optical nanostructures for photovoltaics with near-field scanning microscopy; Untersuchung optischer Nanostrukturen fuer die Photovoltaik mit Nahfeldmikroskopie

    Energy Technology Data Exchange (ETDEWEB)

    Beckers, Thomas

    2011-09-26

    Textured and rough surfaces are known to increase light trapping in solar cells significantly. The development and optimization of these nano-structures is essential to improve the energy conversion efficiency of thin-film solar cells. In the past, first research approaches covered classical and macroscopic investigations, e.g. determining the haze or angularly resolved scattering. These methods do not provide precise explanation for the optical improvement of the devices, because layer thicknesses and structure sizes in thin-film solar cells are smaller than the wavelength of visible light. The impact of local nano-structures and their contribution to the local absorption enhancement is not resolved by macroscopic measurements. In this thesis, near-field scanning optical microscopy is introduced as first near-field investigations of nano-structures for photovoltaics. This provides an insight into local optical effects for relevant surfaces of photovoltaic devices. Investigating the distribution of the electric fields in layer stacks is crucial to understand the absorption in solar cells. Evanescent fields, which occur due to total internal reflection at the interfaces, are measurable by near-field scanning optical microscopy and yield important information about local light trapping. Within the framework of this thesis, correlations between local surface structures and optical near-field effects are shown. In this case structure features of randomly textured surfaces, which optimize local light trapping, are identified. It paves the way to connect microscopic optical effects on the surface with the macroscopic performance of thin-film solar cells. Moreover, the measurement yields a 3D illustration of the electric field distribution over the sample surface. It is an important criterion to prove the results of rigorous diffraction theory. An excellent agreement between experiment and simulation is found. The simulations provide an insight into the material, which is

  2. An adaptive approach for the segmentation and extraction of planar and linear/cylindrical features from laser scanning data

    Science.gov (United States)

    Lari, Zahra; Habib, Ayman

    2014-07-01

    Laser scanning systems have been established as leading tools for the collection of high density three-dimensional data over physical surfaces. The collected point cloud does not provide semantic information about the characteristics of the scanned surfaces. Therefore, different processing techniques have been developed for the extraction of useful information from this data which could be applied for diverse civil, industrial, and military applications. Planar and linear/cylindrical features are among the most important primitive information to be extracted from laser scanning data, especially those collected in urban areas. This paper introduces a new approach for the identification, parameterization, and segmentation of these features from laser scanning data while considering the internal characteristics of the utilized point cloud - i.e., local point density variation and noise level in the dataset. In the first step of this approach, a Principal Component Analysis of the local neighborhood of individual points is implemented to identify the points that belong to planar and linear/cylindrical features and select their appropriate representation model. For the detected planar features, the segmentation attributes are then computed through an adaptive cylinder neighborhood definition. Two clustering approaches are then introduced to segment and extract individual planar features in the reconstructed parameter domain. For the linear/cylindrical features, their directional and positional parameters are utilized as the segmentation attributes. A sequential clustering technique is proposed to isolate the points which belong to individual linear/cylindrical features through directional and positional attribute subspaces. Experimental results from simulated and real datasets demonstrate the feasibility of the proposed approach for the extraction of planar and linear/cylindrical features from laser scanning data.

  3. Comparative Study of Neural Network Frameworks for the Next Generation of Adaptive Optics Systems.

    Science.gov (United States)

    González-Gutiérrez, Carlos; Santos, Jesús Daniel; Martínez-Zarzuela, Mario; Basden, Alistair G; Osborn, James; Díaz-Pernas, Francisco Javier; De Cos Juez, Francisco Javier

    2017-06-02

    Many of the next generation of adaptive optics systems on large and extremely large telescopes require tomographic techniques in order to correct for atmospheric turbulence over a large field of view. Multi-object adaptive optics is one such technique. In this paper, different implementations of a tomographic reconstructor based on a machine learning architecture named "CARMEN" are presented. Basic concepts of adaptive optics are introduced first, with a short explanation of three different control systems used on real telescopes and the sensors utilised. The operation of the reconstructor, along with the three neural network frameworks used, and the developed CUDA code are detailed. Changes to the size of the reconstructor influence the training and execution time of the neural network. The native CUDA code turns out to be the best choice for all the systems, although some of the other frameworks offer good performance under certain circumstances.

  4. Sky coverage modeling for the whole sky for laser guide star multiconjugate adaptive optics.

    Science.gov (United States)

    Wang, Lianqi; Andersen, David; Ellerbroek, Brent

    2012-06-01

    The scientific productivity of laser guide star adaptive optics systems strongly depends on the sky coverage, which describes the probability of finding natural guide stars for the tip/tilt wavefront sensor(s) to achieve a certain performance. Knowledge of the sky coverage is also important for astronomers planning their observations. In this paper, we present an efficient method to compute the sky coverage for the laser guide star multiconjugate adaptive optics system, the Narrow Field Infrared Adaptive Optics System (NFIRAOS), being designed for the Thirty Meter Telescope project. We show that NFIRAOS can achieve more than 70% sky coverage over most of the accessible sky with the requirement of 191 nm total rms wavefront.

  5. Analysis of a holographic laser adaptive optics system using a deformable mirror.

    Science.gov (United States)

    Yao, Kainan; Wang, Jianli; Liu, Xinyue; Lin, Xudong; Chen, Lu

    2017-08-10

    We describe a closed-loop holographic laser adaptive optics system (HLAOS) based on a holographic wavefront sensor (HWFS) and 21-element continuous-surface piezoelectric deformable mirror (DM). The principle behind HWFSs is described, and then the response sensitivity and crosstalk effect on the lowest 12 Zernike modes of aberration are analyzed. Next, the wavefront-correction capability of the 21-element DM is analyzed. The closed-loop correction of the HLAOS to a static aberration is then numerically simulated. We report a practical implementation of the HLAOS and compare the aberration-compensation effect with a traditional adaptive optics system based on a 37-unit Shark-Hartmann sensor. The practically relevant parameters are analyzed and the experimental results show that an HLAOS using a piezoelectric DM can achieve a correction capability comparable to that of a traditional adaptive optics system.

  6. Tuning of successively scanned two monolithic Vernier-tuned lasers and selective data sampling in optical comb swept source optical coherence tomography

    Science.gov (United States)

    Choi, Dong-hak; Yoshimura, Reiko; Ohbayashi, Kohji

    2013-01-01

    Monolithic Vernier tuned super-structure grating distributed Bragg reflector (SSG-DBR) lasers are expected to become one of the most promising sources for swept source optical coherence tomography (SS-OCT) with a long coherence length, reduced sensitivity roll-off, and potential capability for a very fast A-scan rate. However, previous implementations of the lasers suffer from four main problems: 1) frequencies deviate from the targeted values when scanned, 2) large amounts of noise appear associated with abrupt changes in injection currents, 3) optically aliased noise appears due to a long coherence length, and 4) the narrow wavelength coverage of a single chip limits resolution. We have developed a method of dynamical frequency tuning, a method of selective data sampling to eliminate current switching noise, an interferometer to reduce aliased noise, and an excess-noise-free connection of two serially scanned lasers to enhance resolution to solve these problems. An optical frequency comb SS-OCT system was achieved with a sensitivity of 124 dB and a dynamic range of 55-72 dB that depended on the depth at an A-scan rate of 3.1 kHz with a resolution of 15 μm by discretely scanning two SSG-DBR lasers, i.e., L-band (1.560-1.599 μm) and UL-band (1.598-1.640 μm). A few OCT images with excellent image penetration depth were obtained. PMID:24409394

  7. An acousto-optically steered laser scanning system for measurement of action potential spread in intact heart.

    Science.gov (United States)

    Morad, M; Dillon, S; Weiss, J

    1986-01-01

    An optical scanning device that combines a voltage-sensitive dye and an acousto-optically steered He-Ne laser beam is described. This device is capable of scanning 128 sites every 4 ms and recording and storing the fluorescence signals for a duration of up to 1 s (several beats). Comparison of an activation map constructed from laser scanning to those obtained from multiple extracellular electrodes suggests that this technique is highly reliable. Although motion-induced light scattering appears to alter the shape of the action potential, the upstroke can be distinguished quite reliably even in a vigorously contracting muscle. This technique provides high resolution (up to 50 micron) and high flexibility (i.e., the scanned sites can be concentrated over a small or very large area) in measuring the spread of activation in heart muscle. By having only one excitation and one measurement element, the approach offers simplicity and high flexibility to the user. We have shown that this system can be readily applied to the task for which it was intended--probing the mechanisms of arrhythmias in the mammalian myocardium. It has been demonstrated, for example, that arrhythmias due to automaticity can be readily distinguished from those due to reentry through the mapping capability of the laser scanner. In addition, the ability of laser scanner to measure membrane depolarization directly during arrhythmias may make this technique superior to conventional electrocardiographic mapping techniques.

  8. Particle size distributions determined by optical scanning and by sieving in the assessment of masticatory performance of complete denture wearers.

    Science.gov (United States)

    Eberhard, Lydia; Schneider, Sophie; Eiffler, Constantin; Kappel, Stefanie; Giannakopoulos, Nikolaos Nikitas

    2015-03-01

    Standard procedure for the measurement of masticatory performance is the fractionated sieving of fragmented test food, which is substantially time consuming. The aim of this study was to introduce a less laborious, comparable, and valid technique based on scanning. Fifty-six Optocal chewing samples were minced by wearers of complete dentures with 15 and 40 chewing strokes and analyzed by both a sieving and a scanning method. The sieving procedure was carried out using ten sieves (5.6, 4.0, 2.8, 2.0, 1.4, 1.0, 0.71, 0.5, 0.355, and 0.25 mm) and measuring the weight of the specific fractions. Scanning was performed with a flatbed scanner (Epson Expression1600Pro, Seiko Epson Corporation, Japan, 1,200 dpi). Scanned images underwent image analysis (ImageJ 1.42q, NIH, USA), which yielded descriptive parameters for each particle. Out of the 2D image, a volume was estimated for each particle. In order to receive a discrete particle size distribution, area-volume-conversion factors were determined. The cumulated weights yielded by either method were curve fitted with the Rosin-Rammler distribution (MATLAB, The MathWorks, Inc., Natick, USA) to determine the median particle size X 50. The Rosin-Rammler distributions for sieving and scanning resembled each other and showed an excellent correlation in 15/40 chewing strokes (r = 0.995/r = 0.971, P sieving. On average, scanning overestimated the X 50 values by 1/2.4 %. The scanning method took 10 min per sample in contrast to 50 min for sieving. Optical scanning is a valid method comparable to sieving. The described method is feasible and appropriate for the measurement of masticatory performance of denture wearers.

  9. Radio over fiber link with adaptive order n‐QAM optical phase modulated OFDM and digital coherent detection

    DEFF Research Database (Denmark)

    Arlunno, Valeria; Borkowski, Robert; Guerrero Gonzalez, Neil

    2011-01-01

    Successful digital coherent demodulation of asynchronous optical phase‐modulated adaptive order QAM (4, 16, and 64) orthogonal frequency division multiplexing signals is achieved by a single reconfigurable digital receiver after 78 km of optical deployed fiber transmission.......Successful digital coherent demodulation of asynchronous optical phase‐modulated adaptive order QAM (4, 16, and 64) orthogonal frequency division multiplexing signals is achieved by a single reconfigurable digital receiver after 78 km of optical deployed fiber transmission....

  10. Compact infrared camera (CIRC) for earth observation adapting athermal optics

    Science.gov (United States)

    Kato, Eri; Katayama, Haruyoshi; Naitoh, Masataka; Harada, Masatomo; Nakamura, Ryoko; Nakau, Koji; Sato, Ryota

    2013-09-01

    We have developed the compact infrared camera (CIRC) with an uncooled infrared array detector (microbolometer) for space application. The main mission of the CIRC is the technology demonstration of the wildfire detection using a large format (640×480) microbolometer. Wildfires are major and chronic disasters affecting numerous countries, especially in the Asia-Pacific region, and may get worse with global warming and climate change. Microbolometers have an advantage of not requiring cooling systems such an a mechanical cooler, and is suitable for resource-limited sensor systems or small satellites. Main characteristic of the CIRC is also an athermal optics. The thermal optics compensates the defocus due to the temperature change by using Germanium and Chalcogenide glass which have different coefficient of thermal expansion and temperature dependence of refractive index. The CIRC achieves a small size, light weight, and low electrical power by employing the athermal optics and a shutter-less system. Two CIRCs will be carried as a technology demonstration payload of ALOS-2 and JEM-CALET, which will be launched in JFY 2013 and 2014, respectively. We have finished the ground calibration test of the CIRC Proto Flight Model (PFM). Athermal optical performance of the CIRC have been confirmed by measuring modulation transfer function (MTF) in a vacuum environment and at environmental temperature from -15 to 50 °C. As a result, MTF was found to be effective at capturing clear images across the entire range of operating temperatures. We also provide an overview of the CIRC and radiometric test results in this presentation.

  11. Adapted Treatment Guided by Interim PET-CT Scan in Advanced Hodgkin's Lymphoma.

    Science.gov (United States)

    Johnson, Peter; Federico, Massimo; Kirkwood, Amy; Fosså, Alexander; Berkahn, Leanne; Carella, Angelo; d'Amore, Francesco; Enblad, Gunilla; Franceschetto, Antonella; Fulham, Michael; Luminari, Stefano; O'Doherty, Michael; Patrick, Pip; Roberts, Thomas; Sidra, Gamal; Stevens, Lindsey; Smith, Paul; Trotman, Judith; Viney, Zaid; Radford, John; Barrington, Sally

    2016-06-23

    We tested interim positron-emission tomography-computed tomography (PET-CT) as a measure of early response to chemotherapy in order to guide treatment for patients with advanced Hodgkin's lymphoma. Patients with newly diagnosed advanced classic Hodgkin's lymphoma underwent a baseline PET-CT scan, received two cycles of ABVD (doxorubicin, bleomycin, vinblastine, and dacarbazine) chemotherapy, and then underwent an interim PET-CT scan. Images were centrally reviewed with the use of a 5-point scale for PET findings. Patients with negative PET findings after two cycles were randomly assigned to continue ABVD (ABVD group) or omit bleomycin (AVD group) in cycles 3 through 6. Those with positive PET findings after two cycles received BEACOPP (bleomycin, etoposide, doxorubicin, cyclophosphamide, vincristine, procarbazine, and prednisone). Radiotherapy was not recommended for patients with negative findings on interim scans. The primary outcome was the difference in the 3-year progression-free survival rate between randomized groups, a noninferiority comparison to exclude a difference of 5 or more percentage points. A total of 1214 patients were registered; 937 of the 1119 patients (83.7%) who underwent an interim PET-CT scan according to protocol had negative findings. With a median follow-up of 41 months, the 3-year progression-free survival rate and overall survival rate in the ABVD group were 85.7% (95% confidence interval [CI], 82.1 to 88.6) and 97.2% (95% CI, 95.1 to 98.4), respectively; the corresponding rates in the AVD group were 84.4% (95% CI, 80.7 to 87.5) and 97.6% (95% CI, 95.6 to 98.7). The absolute difference in the 3-year progression-free survival rate (ABVD minus AVD) was 1.6 percentage points (95% CI, -3.2 to 5.3). Respiratory adverse events were more severe in the ABVD group than in the AVD group. BEACOPP was given to the 172 patients with positive findings on the interim scan, and 74.4% had negative findings on a third PET-CT scan; the 3-year progression

  12. Adapted Treatment Guided by Interim PET-CT Scan in Advanced Hodgkin’s Lymphoma

    Science.gov (United States)

    Johnson, Peter; Federico, Massimo; Kirkwood, Amy; Fosså, Alexander; Berkahn, Leanne; Carella, Angelo; d’Amore, Francesco; Enblad, Gunilla; Franceschetto, Antonella; Fulham, Michael; Luminari, Stefano; O’Doherty, Michael; Patrick, Pip; Roberts, Thomas; Sidra, Gamal; Stevens, Lindsey; Smith, Paul; Trotman, Judith; Viney, Zaid; Radford, John; Barrington, Sally

    2016-01-01

    Background We tested interim positron-emission tomography–computed tomography (PET-CT) as a measure of early response to chemotherapy in order to guide treatment for patients with advanced Hodgkin’s lymphoma. Methods Patients with newly diagnosed advanced classic Hodgkin’s lymphoma underwent a baseline PET-CT scan, received two cycles of ABVD (doxorubicin, bleomycin, vinblastine, and dacarbazine) chemotherapy, and then underwent an interim PET-CT scan. Images were centrally reviewed with the use of a 5-point scale for PET findings. Patients with negative PET findings after two cycles were randomly assigned to continue ABVD (ABVD group) or omit bleomycin (AVD group) in cycles 3 through 6. Those with positive PET findings after two cycles received BEACOPP (bleomycin, etoposide, doxorubicin, cyclophosphamide, vincristine, procarbazine, and prednisone). Radiotherapy was not recommended for patients with negative findings on interim scans. The primary outcome was the difference in the 3-year progression-free survival rate between randomized groups, a noninferiority comparison to exclude a difference of 5 or more percentage points. Results A total of 1214 patients were registered; 937 of the 1119 patients (83.7%) who underwent an interim PET-CT scan according to protocol had negative findings. With a median follow-up of 41 months, the 3-year progression-free survival rate and overall survival rate in the ABVD group were 85.7% (95% confidence interval [CI], 82.1 to 88.6) and 97.2% (95% CI, 95.1 to 98.4), respectively; the corresponding rates in the AVD group were 84.4% (95% CI, 80.7 to 87.5) and 97.6% (95% CI, 95.6 to 98.7). The absolute difference in the 3-year progression-free survival rate (ABVD minus AVD) was 1.6 percentage points (95% CI, −3.2 to 5.3). Respiratory adverse events were more severe in the ABVD group than in the AVD group. BEACOPP was given to the 172 patients with positive findings on the interim scan, and 74.4% had negative findings on a third

  13. Microfabricated optical fiber with microlens that produces large field-of-view video-rate optical beam scanning for microendoscopy applications

    Science.gov (United States)

    Seibel, Eric J.; Fauver, Mark; Crossman-Bosworth, Janet L.; Smithwick, Quinn Y. J.; Brown, Chris M.

    2003-07-01

    Our goal is to produce a micro-optical scanner at the tip of an ultrathin flexible endoscope with an overall diameter of 1 mm. Using a small diameter piezoelectric tube actuator, a cantilevered optical fiber can be driven in mechanical resonance to scan a beam of light in a space-filling, spiral scan pattern. By knowing and/or controlling the fiber position and acquiring backscattered intensity with a photodetector, an image is acquired. A microfabrication process of computer-controlled acid etching is used to reduce the mass along the fiber scanner shaft to allow for high scan amplitude and frequency. A microlens (50 degrees full angle), up to video scan rates (>10 KHz), while maintaining a scanner diameter of 1 mm. A comparison can be made to bi-axial mirror scanners being fabricated as a MEMS device (micro-electro-mechanical system). Based on the opto-mechanical performance of these microlensed fiber scanners, flexible catheter scopes are possible for new microendoscopies that combine imaging with laser diagnoses.

  14. Benefit of adaptive FEC in shared backup path protected elastic optical network.

    Science.gov (United States)

    Guo, Hong; Dai, Hua; Wang, Chao; Li, Yongcheng; Bose, Sanjay K; Shen, Gangxiang

    2015-07-27

    We apply an adaptive forward error correction (FEC) allocation strategy to an Elastic Optical Network (EON) operated with shared backup path protection (SBPP). To maximize the protected network capacity that can be carried, an Integer Linear Programing (ILP) model and a spectrum window plane (SWP)-based heuristic algorithm are developed. Simulation results show that the FEC coding overhead required by the adaptive FEC scheme is significantly lower than that needed by a fixed FEC allocation strategy resulting in higher network capacity for the adaptive strategy. The adaptive FEC allocation strategy can also significantly outperform the fixed FEC allocation strategy both in terms of the spare capacity redundancy and the average FEC coding overhead needed per optical channel. The proposed heuristic algorithm is efficient and not only performs closer to the ILP model but also does much better than the shortest-path algorithm.

  15. Simultaneous multimodal ophthalmic imaging using swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography

    Science.gov (United States)

    Malone, Joseph D.; El-Haddad, Mohamed T.; Bozic, Ivan; Tye, Logan A.; Majeau, Lucas; Godbout, Nicolas; Rollins, Andrew M.; Boudoux, Caroline; Joos, Karen M.; Patel, Shriji N.; Tao, Yuankai K.

    2016-01-01

    Scanning laser ophthalmoscopy (SLO) benefits diagnostic imaging and therapeutic guidance by allowing for high-speed en face imaging of retinal structures. When combined with optical coherence tomography (OCT), SLO enables real-time aiming and retinal tracking and provides complementary information for post-acquisition volumetric co-registration, bulk motion compensation, and averaging. However, multimodality SLO-OCT systems generally require dedicated light sources, scanners, relay optics, detectors, and additional digitization and synchronization electronics, which increase system complexity. Here, we present a multimodal ophthalmic imaging system using swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography (SS-SESLO-OCT) for in vivo human retinal imaging. SESLO reduces the complexity of en face imaging systems by multiplexing spatial positions as a function of wavelength. SESLO image quality benefited from single-mode illumination and multimode collection through a prototype double-clad fiber coupler, which optimized scattered light throughput and reduce speckle contrast while maintaining lateral resolution. Using a shared 1060 nm swept-source, shared scanner and imaging optics, and a shared dual-channel high-speed digitizer, we acquired inherently co-registered en face retinal images and OCT cross-sections simultaneously at 200 frames-per-second. PMID:28101411

  16. AVES-IMCO: an adaptive optics visible spectrograph and imager/coronograph for NAOS

    Science.gov (United States)

    Beuzit, Jean-Luc; Lagrange, A.-M.; Mouillet, D.; Chauvin, G.; Stadler, E.; Charton, J.; Lacombe, F.; AVES-IMCO Team

    2001-05-01

    The NAOS adaptive optics system will very soon provide diffraction-limited images on the VLT, down to the visible wavelengths (0.020 arcseconds at 0.83 micron for instance). At the moment, the only instrument dedicated to NAOS is the CONICA spectro-imager, operating in the near-infrared from 1 to 5 microns. We are now proposing to ESO, in collaboration with an Italian group, the development of a visible spectrograph/imager/coronograph, AVES-IMCO (Adaptive Optics Visual Echelle Spectrograph and IMager/COronograph). We present here the general concept of the new instrument as well as its expected performances in the different modes.

  17. Fixation light hue bias revisited: implications for using adaptive optics to study color vision.

    Science.gov (United States)

    Hofer, H J; Blaschke, J; Patolia, J; Koenig, D E

    2012-03-01

    Current vision science adaptive optics systems use near infrared wavefront sensor 'beacons' that appear as red spots in the visual field. Colored fixation targets are known to influence the perceived color of macroscopic visual stimuli (Jameson, D., & Hurvich, L. M. (1967). Fixation-light bias: An unwanted by-product of fixation control. Vision Research, 7, 805-809.), suggesting that the wavefront sensor beacon may also influence perceived color for stimuli displayed with adaptive optics. Despite its importance for proper interpretation of adaptive optics experiments on the fine scale interaction of the retinal mosaic and spatial and color vision, this potential bias has not yet been quantified or addressed. Here we measure the impact of the wavefront sensor beacon on color appearance for dim, monochromatic point sources in five subjects. The presence of the beacon altered color reports both when used as a fixation target as well as when displaced in the visual field with a chromatically neutral fixation target. This influence must be taken into account when interpreting previous experiments and new methods of adaptive correction should be used in future experiments using adaptive optics to study color. Copyright © 2012 Elsevier Ltd. All rights reserved.

  18. Design of co-path scanning long trace profiler for measurement of x-ray space optical elements

    Science.gov (United States)

    Shun, Li; Yan, Gong; Wei, Zhang; Yang, Zhao

    2010-08-01

    The Long Trace Profiler (LTP) is a precision surface slope error measurement instrument used in synchrotron radiation optics for many years. By making some modifications to the LTP system, we developed a co-path scanning LTP (CSLTP) system to test the cylindrical aspherical surface which used in X-ray space optics. To reduce the mistake caused by air turbulence and manufacture faults of optical elements used, the CSLTP is designed with the least difference between the testing beam path and the reference beam path. Also, it uses multiple-beam interference but double beam interference to reduce the width of beam fringe. This improves the position precision of the beam fringe on the image plane.

  19. Sodium Laser Guide Star Adaptive Optics Imaging Polarimetry of Herbig Ae/Be Stars

    Energy Technology Data Exchange (ETDEWEB)

    Perrin, M D; Graham, J R; Lloyd, J P; Kalas, P; Gates, E L; Gavel, D T; Pennington, D M; Max, C E

    2004-01-08

    The future of high-resolution ground-based optical and infrared astronomy requires the successful implementation of laser guide star adaptive optics systems. We present the first science results from the Lick Observatory sodium beacon laser guide star system. By coupling this system to a near-infrared (J;H;Ks bands) dual-channel imaging polarimeter, we achieve very high sensitivity to light scattered in the circumstellar enviroment of Herbig Ae/Be stars on scales of 100-300 AU. Observations of LkH{alpha} 198 reveal a highly polarized, biconical nebula 10 arcseconds in diameter (6000 AU) . We also observe a polarized jet-like feature associated with the deeply embedded source LkH{alpha} 198-IR. The star LkH{alpha} 233 presents a narrow, unpolarized dark lane dividing its characteristic butterfly-shaped polarized reflection nebulosity. This linear structure is oriented perpendicular to an optical jet and bipolar cavity and is consistent with the presence of an optically thick circumstellar disk blocking our direct view of the star. These data suggest that the evolutionary picture developed for the lower-mass T Tauri stars is also relevant to the Herbig Ae/Be stars and demonstrate the ability of laser guide star adaptive optics systems to obtain scientific results competitive with natural guide star adaptive optics or space-based telescopes.

  20. Towards feasible and effective predictive wavefront control for adaptive optics

    Energy Technology Data Exchange (ETDEWEB)

    Poyneer, L A; Veran, J

    2008-06-04

    We have recently proposed Predictive Fourier Control, a computationally efficient and adaptive algorithm for predictive wavefront control that assumes frozen flow turbulence. We summarize refinements to the state-space model that allow operation with arbitrary computational delays and reduce the computational cost of solving for new control. We present initial atmospheric characterization using observations with Gemini North's Altair AO system. These observations, taken over 1 year, indicate that frozen flow is exists, contains substantial power, and is strongly detected 94% of the time.

  1. Automatic detection of Hyperreflective Foci in optical coherence tomography B-scans using Morphological Component Analysis.

    Science.gov (United States)

    Mokhtari, Marzieh; Ghasemi Kamasi, Zeinab; Rabbani, Hossein

    2017-07-01

    Hyperreflective Foci (HF) is one of the most common complications distributed in cross-sectional images of patients with Diabetic Macular Edema (DME). Scanning Laser Ophthalmoscope (SLO) images usually consists of several B-scans that represent a cross-sectional reconstruction of a plane through the anterior or posterior regions of retina. In each B-scan, HFs are geometrically distinct constituents in different retinal layers. Since the intensity levels of HFs and many other subjects in B-scans are the same, in this paper we try to separate HFs from other objects by detection of the point and curve singularities in each B-scan. The decomposition algorithm presented in this paper is based on sparse image representation of B-scans using Morphological Component Analysis (MCA) technique. By using curvelet transform and Daubechies wavelet basis, two different over-complete dictionaries are constructed which represent two various aspects of B-scans. The HFs are more distinguished in reconstructed image with wavelet dictionary and other objects are mostly detectable by curvelet dictionary. So, HFs can be detected by applying an optimum threshold criterion on reconstructed image by wavelet atoms. Finally, the false positive points are reduced by removing the candidate points in RNFL and RPE layers, which are automatically segmented based on ridgelet transform. Our simulation results on 1924 HFs show that sensitivity and specificity for HF detection is 91.0% and 100%, respectively.

  2. Replica calibration artefacts for optical 3D scanning of micro parts

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo; Carmignato, S.; Cantatore, Angela

    2009-01-01

    applications to reproduce the geometry of a step artefact, a miniature step gauge, and a curve standard for optical measuring machines. The replica artefacts were calibrated using a tactile coordinate measuring machine and measured on two different optical scanners. Replication quality and applicability...

  3. Polarization-sensitive spectral-domain optical coherence tomography using a single line scan camera

    NARCIS (Netherlands)

    Cense, B.; Mujat, M.; Chen, T.; Park, B. H.; de Boer, J.F.

    2007-01-01

    Polarization-sensitive optical coherence tomography can be used to measure the birefringence of biological tissue such as the human retina. Previous measurements with a time-domain polarization-sensitive optical coherence tomography system revealed that the birefringence of the human retinal nerve

  4. Three-dimensional manipulation with scanning near-field optical nanotweezers.

    Science.gov (United States)

    Berthelot, J; Aćimović, S S; Juan, M L; Kreuzer, M P; Renger, J; Quidant, R

    2014-04-01

    Recent advances in nanotechnologies have prompted the need for tools to accurately and non-invasively manipulate individual nano-objects. Among the possible strategies, optical forces have been predicted to provide researchers with nano-optical tweezers capable of trapping a specimen and moving it in three dimensions. In practice, however, the combination of weak optical forces and photothermal issues has thus far prevented their experimental realization. Here, we demonstrate the first three-dimensional optical manipulation of single 50 nm dielectric objects with near-field nanotweezers. The nano-optical trap is built by engineering a bowtie plasmonic aperture at the extremity of a tapered metal-coated optical fibre. Both the trapping operation and monitoring are performed through the optical fibre, making these nanotweezers totally autonomous and free of bulky optical elements. The achieved trapping performances allow for the trapped specimen to be moved over tens of micrometres over a period of several minutes with very low in-trap intensities. This non-invasive approach is foreseen to open new horizons in nanosciences by offering an unprecedented level of control of nanosized objects, including heat-sensitive biospecimens.

  5. Laser based bi-directional Gbit ground links with the Tesat transportable adaptive optical ground station

    Science.gov (United States)

    Heine, Frank; Saucke, Karen; Troendle, Daniel; Motzigemba, Matthias; Bischl, Hermann; Elser, Dominique; Marquardt, Christoph; Henninger, Hennes; Meyer, Rolf; Richter, Ines; Sodnik, Zoran

    2017-02-01

    Optical ground stations can be an alternative to radio frequency based transmit (forward) and receive (return) systems for data relay services and other applications including direct to earth optical communications from low earth orbit spacecrafts, deep space receivers, space based quantum key distribution systems and Tbps capacity feeder links to geostationary spacecrafts. The Tesat Transportable Adaptive Optical Ground Station is operational since September 2015 at the European Space Agency site in Tenerife, Spain.. This paper reports about the results of the 2016 experimental campaigns including the characterization of the optical channel from Tenerife for an optimized coding scheme, the performance of the T-AOGS under different atmospheric conditions and the first successful measurements of the suitability of the Alphasat LCT optical downlink performance for future continuous variable quantum key distribution systems.

  6. Comparative study of infrared wavefront sensing solutions for adaptive optics

    Science.gov (United States)

    Plantet, C.; Fusco, T.; Guerineau, N.; Derelle, S.; Robert, C.

    2016-07-01

    The development of new low-noise infrared detectors, such as RAPID (CEA LETI/Sofradir) or SAPHIRA (Selex), has given the possibility to consider infrared wavefront sensing at low ux. We propose here a comparative study of near infrared (J and H bands) wavefront sensing concepts for mid and high orders estimation on a 8m- class telescope, relying on three existing wavefront sensors: the Shack-Hartmann sensor, the pyramid sensor and the quadri-wave lateral shearing interferometer. We consider several conceptual designs using the RAPID camera, making a trade-off between background flux, optical thickness and compatibility with a compact cryostat integration. We then study their sensitivity to noise in order to compare them in different practical scenarios. The pyramid provides the best performance, with a gain up to 0.5 magnitude, and has an advantageous setup.

  7. Adaptive spectral window sizes for extraction of diagnostic features from optical spectra

    Science.gov (United States)

    Kan, Chih-Wen; Lee, Andy Y.; Nieman, Linda T.; Sokolov, Konstantin; Markey, Mia K.

    2010-07-01

    We present an approach to adaptively adjust the spectral window sizes for optical spectra feature extraction. Previous studies extracted features from spectral windows of a fixed width. In our algorithm, piecewise linear regression is used to adaptively adjust the window sizes to find the maximum window size with reasonable linear fit with the spectrum. This adaptive windowing technique ensures the signal linearity in defined windows; hence, the adaptive windowing technique retains more diagnostic information while using fewer windows. This method was tested on a data set of diffuse reflectance spectra of oral mucosa lesions. Eight features were extracted from each window. We performed classifications using linear discriminant analysis with cross-validation. Using windowing techniques results in better classification performance than not using windowing. The area under the receiver-operating-characteristics curve for windowing techniques was greater than a nonwindowing technique for both normal versus mild dysplasia (MD) plus severe high-grade dysplasia or carcinama (SD) (MD+SD) and benign versus MD+SD. Although adaptive and fixed-size windowing perform similarly, adaptive windowing utilizes significantly fewer windows than fixed-size windows (number of windows per spectrum: 8 versus 16). Because adaptive windows retain most diagnostic information while reducing the number of windows needed for feature extraction, our results suggest that it isolates unique diagnostic features in optical spectra.

  8. Non-destructive 3D imaging of composite restorations using optical coherence tomography: marginal adaptation of self-etch adhesives.

    Science.gov (United States)

    Makishi, Patricia; Shimada, Yasushi; Sadr, Alireza; Tagami, Junji; Sumi, Yasunori

    2011-04-01

    To investigate the potential use of swept-source optical coherence tomography (SS-OCT) as a new tool to evaluate marginal adaptation of composite restorations in class I cavities. Round-shaped class I cavities (3mm diameter × 1.5mm depth) were prepared on buccal enamel of bovine teeth with cavity floor located in dentine. The cavities were restored with a flowable resin composite (Clearfil Majesty LV) using two-step self-etch adhesive (SE Bond), all-in-one self-etch adhesive (G-Bond) or no adhesive (Control). The specimens were subjected to water storage (37 °C, 24 h) or thermal stress challenge (5000 cycles, 5 °C and 55 °C). 3D scans (4 mm×4 mm×2.6 mm obtained in 4 s) of the restoration were obtained using SS-OCT before and after immersion into a contrast agent. 2D images obtained from the 3D scans (n=30/group) were analysed to evaluate marginal adaptation. Area fraction of pixels with high brightness values at the interfacial zone was calculated using a digital image analysis software. The results were statistically compared with statistical significance defined as p≤0.05. Wilcoxon signed ranks test showed that there was no statistically significant difference in the results of SS-OCT before and after infiltration of the contrast agent when a ranking transformation was applied on to the data (p>0.05). A significant positive linear correlation was found between the two SS-OCT images. Confocal laser scanning photomicrographs of samples cut after silver infiltration confirmed the presence of gap. 3D imaging by SS-OCT can be considered as a non-invasive technique for fast detection of gaps at the restoration interface. Copyright © 2011 Elsevier Ltd. All rights reserved.

  9. Graphics processing unit accelerated optical coherence tomography processing at megahertz axial scan rate and high resolution video rate volumetric rendering.

    Science.gov (United States)

    Jian, Yifan; Wong, Kevin; Sarunic, Marinko V

    2013-02-01

    In this report, we describe how to highly optimize a computer unified device architecture based platform to perform real-time processing of optical coherence tomography interferometric data and three-dimensional (3-D) volumetric rendering using a commercially available, cost-effective, graphics processing unit (GPU). The maximum complete attainable axial scan processing rate, including memory transfer and displaying B-scan frame, was 2.24 MHz for 16 bits pixel depth and 2048 fast Fourier transform size; the maximum 3-D volumetric rendering rate, including B-scan, en face view display, and 3-D rendering, was ~23 volumes/second (volume size: 1024×256×200). To the best of our knowledge, this is the fastest processing rate reported to date with a single-chip GPU and the first implementation of real-time video-rate volumetric optical coherence tomography (OCT) processing and rendering that is capable of matching the acquisition rates of ultrahigh-speed OCT.

  10. Graphics processing unit accelerated optical coherence tomography processing at megahertz axial scan rate and high resolution video rate volumetric rendering

    Science.gov (United States)

    Jian, Yifan; Wong, Kevin; Sarunic, Marinko V.

    2013-02-01

    In this report, we describe how to highly optimize a computer unified device architecture based platform to perform real-time processing of optical coherence tomography interferometric data and three-dimensional (3-D) volumetric rendering using a commercially available, cost-effective, graphics processing unit (GPU). The maximum complete attainable axial scan processing rate, including memory transfer and displaying B-scan frame, was 2.24 MHz for 16 bits pixel depth and 2048 fast Fourier transform size; the maximum 3-D volumetric rendering rate, including B-scan, en face view display, and 3-D rendering, was ˜23 volumes/second (volume size: 1024×256×200). To the best of our knowledge, this is the fastest processing rate reported to date with a single-chip GPU and the first implementation of real-time video-rate volumetric optical coherence tomography (OCT) processing and rendering that is capable of matching the acquisition rates of ultrahigh-speed OCT.

  11. Optical design and multi-length-scale scanning spectro-microscopy possibilities at the Nanoscopium beamline of Synchrotron Soleil.

    Science.gov (United States)

    Somogyi, Andrea; Medjoubi, Kadda; Baranton, Gil; Le Roux, Vincent; Ribbens, Marc; Polack, François; Philippot, Pascal; Samama, Jean Pierre

    2015-07-01

    The Nanoscopium 155 m-long beamline of Synchrotron Soleil is dedicated to scanning hard X-ray nanoprobe techniques. Nanoscopium aims to reach ≤100 nm resolution in the 5-20 keV energy range for routine user experiments. The beamline design tackles the tight stability requirements of such a scanning nanoprobe by creating an overfilled secondary source, implementing all horizontally reflecting main beamline optics, applying high mechanical stability equipment and constructing a dedicated high-stability building envelope. Multi-technique scanning imaging and tomography including X-ray fluorescence spectrometry and spectro-microscopy, absorption, differential phase and dark-field contrasts are implemented at the beamline in order to provide simultaneous information on the elemental distribution, speciation and sample morphology. This paper describes the optical concept and the first measured performance of the Nanoscopium beamline followed by the hierarchical length-scale multi-technique imaging experiments performed with dwell times down to 3 ms per pixel.

  12. Multimodal backside imaging of a microcontroller using confocal laser scanning and optical-beam-induced current imaging

    Science.gov (United States)

    Finkeldey, Markus; Göring, Lena; Schellenberg, Falk; Brenner, Carsten; Gerhardt, Nils C.; Hofmann, Martin

    2017-02-01

    Microscopy imaging with a single technology is usually restricted to a single contrast mechanism. Multimodal imaging is a promising technique to improve the structural information that could be obtained about a device under test (DUT). Due to the different contrast mechanisms of laser scanning microscopy (LSM), confocal laser scanning microscopy (CLSM) and optical beam induced current microscopy (OBICM), a combination could improve the detection of structures in integrated circuits (ICs) and helps to reveal their layout. While OBIC imaging is sensitive to the changes between differently doped areas and to semiconductor-metal transitions, CLSM imaging is mostly sensitive to changes in absorption and reflection. In this work we present the implementation of OBIC imaging into a CLSM. We show first results using industry standard Atmel microcontrollers (MCUs) with a feature size of about 250nm as DUTs. Analyzing these types of microcontrollers helps to improve in the field of side-channel attacks to find hardware Trojans, possible spots for laser fault attacks and for reverse engineering. For the experimental results the DUT is placed on a custom circuit board that allows us to measure the current while imaging it in our in-house built stage scanning microscope using a near infrared (NIR) laser diode as light source. The DUT is thinned and polished, allowing backside imaging through the Si-substrate. We demonstrate the possibilities using this optical setup by evaluating OBIC, LSM and CLSM images above and below the threshold of the laser source.

  13. Development of a Low-order Adaptive Optics System at Udaipur ...

    Indian Academy of Sciences (India)

    Abstract. A low-order Adaptive Optics (AO) system is being develo- ped at the Udaipur Solar Observatory and we present in this paper the status of the project, which includes the image stabilization system and calibration of wavefront sensor and deformable mirror. The image stabi- lization system comprises of a piezo ...

  14. Focal-plane wavefront sensing with high-order adaptive optics systems

    NARCIS (Netherlands)

    Korkiakoski, V.; Keller, C.U.; Doelman, N.J.; Kenworthy, M.; Otten, G.; Verhaegen, M.

    2014-01-01

    We investigate methods to calibrate the non-common path aberrations at an adaptive optics system having a wavefront-correcting device working with an extremely high resolution (larger than 150x150 correcting elements). We use focal-plane images collected successively, the corresponding

  15. Development of a scalable generic platform for adaptive optics real time control

    Science.gov (United States)

    Surendran, Avinash; Burse, Mahesh P.; Ramaprakash, A. N.; Parihar, Padmakar

    2015-06-01

    The main objective of the present project is to explore the viability of an adaptive optics control system based exclusively on Field Programmable Gate Arrays (FPGAs), making strong use of their parallel processing capability. In an Adaptive Optics (AO) system, the generation of the Deformable Mirror (DM) control voltages from the Wavefront Sensor (WFS) measurements is usually through the multiplication of the wavefront slopes with a predetermined reconstructor matrix. The ability to access several hundred hard multipliers and memories concurrently in an FPGA allows performance far beyond that of a modern CPU or GPU for tasks with a well-defined structure such as Adaptive Optics control. The target of the current project is to generate a signal for a real time wavefront correction, from the signals coming from a Wavefront Sensor, wherein the system would be flexible to accommodate all the current Wavefront Sensing techniques and also the different methods which are used for wavefront compensation. The system should also accommodate for different data transmission protocols (like Ethernet, USB, IEEE 1394 etc.) for transmitting data to and from the FPGA device, thus providing a more flexible platform for Adaptive Optics control. Preliminary simulation results for the formulation of the platform, and a design of a fully scalable slope computer is presented.

  16. Neptune’s zonal winds from near-IR Keck adaptive optics imaging in August 2001

    NARCIS (Netherlands)

    Martin, S.C.; De Pater, I.; Marcus, P.

    2011-01-01

    We present H-band (1.4–1.8 ?m) images of Neptune with a spatial resolution of ?0.06?, taken with the W.M. Keck II telescope using the slit-viewing camera (SCAM) of the NIRSPEC instrument backed with Adaptive Optics. Images with 60-second integration times span 4 hours each on UT 20 and 21 August,

  17. Statistics of adaptive optics speckles: From probability cloud to probability density function

    OpenAIRE

    Yaitskova, Natalia; Gladysz, Szymon

    2016-01-01

    The complex amplitude in the focal plane of adaptive optics system is modelled as an elliptical complex random variable. The geometrical properties of the probability density function of such variable relate directly to the statistics of the residual phase. Building solely on the twodimensional geometry, the expression for the probability density function of speckle intensity is derived.

  18. A pilot study on slit lamp-adapted optical coherence tomography imaging of trabeculectomy filtering blebs.

    NARCIS (Netherlands)

    Theelen, T.; Wesseling, P.; Keunen, J.E.E.; Klevering, B.J.

    2007-01-01

    BACKGROUND: Our study aims to identify anatomical characteristics of glaucoma filtering blebs by means of slit lamp-adapted optical coherence tomography (SL-OCT) and to identify new parameters for the functional prognosis of the filter in the early post-operative period. METHODS: Patients with

  19. Using 50-mm electrostatic membrane deformable mirror in astronomical adaptive optics

    NARCIS (Netherlands)

    Tokovinin, A.; Thomas, S.; Vdovin, G.

    2004-01-01

    Membrane micro-machined deformable mirrors (MMDM) feature low cost, low power consumption, small size and absence of hysteresis. Interested in using such a device for the adaptive optics system at the SOAR 4.1-m telescope, we evaluated the performance of a 79-channel 50-mm (pupil size 35mm) MMDM

  20. Adaptive optics in spinning disk microscopy: improved contrast and brightness by a simple and fast method.

    Science.gov (United States)

    Fraisier, V; Clouvel, G; Jasaitis, A; Dimitrov, A; Piolot, T; Salamero, J

    2015-09-01

    Multiconfocal microscopy gives a good compromise between fast imaging and reasonable resolution. However, the low intensity of live fluorescent emitters is a major limitation to this technique. Aberrations induced by the optical setup, especially the mismatch of the refractive index and the biological sample itself, distort the point spread function and further reduce the amount of detected photons. Altogether, this leads to impaired image quality, preventing accurate analysis of molecular processes in biological samples and imaging deep in the sample. The amount of detected fluorescence can be improved with adaptive optics. Here, we used a compact adaptive optics module (adaptive optics box for sectioning optical microscopy), which was specifically designed for spinning disk confocal microscopy. The module overcomes undesired anomalies by correcting for most of the aberrations in confocal imaging. Existing aberration detection methods require prior illumination, which bleaches the sample. To avoid multiple exposures of the sample, we established an experimental model describing the depth dependence of major aberrations. This model allows us to correct for those aberrations when performing a z-stack, gradually increasing the amplitude of the correction with depth. It does not require illumination of the sample for aberration detection, thus minimizing photobleaching and phototoxicity. With this model, we improved both signal-to-background ratio and image contrast. Here, we present comparative studies on a variety of biological samples. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

  1. Investigation of third-order nonlinear optical properties of NNDC-doped PMMA thin films by Z-scan technique

    Science.gov (United States)

    Chen, Jingwei; Wang, Xinqiang; Ren, Quan; Patil, P. S.; Li, Tingbin; Yang, Hongliang; Zhang, Jingnan; Li, Guochao; Zhu, Luyi

    2011-11-01

    A novel chalcone derivative, (2 E)-1-(2,4-di- chloro-5-fluorophenyl)-3-[4-dimethylamino)phenyl]prop-2-en-1-one, abbreviated as NNDC, was prepared and characterized by elemental analyses, infrared (IR) and proton nuclear magnetic resonance (1H NMR) spectrum, and thermal analyses. The NNDC-doped poly(methyl methacrylate) (PMMA) thin films with five different doping concentrations by weight were prepared by using a spin-coating method. Their linear optical properties were investigated by using a prism coupling measuring system. The third-order nonlinear optical properties of NNDC in 1,2-dichloroethane (NNDC/1,2-dichloroethane) solution and NNDC-doped PMMA (NNDC/PMMA) films were investigated by using the laser Z-scan technique with 20 ps pulses at 532 nm. A self-focusing effect was observed from the Z-scan curves for solution and thin films and the nonlinear refractive index of the film increases with the increase of the doping concentration. In addition, nonlinear absorption was negligible for all samples. The magnitude of third-order nonlinear refraction index n 2 and the third-order nonlinear susceptibility χ (3) for thin films were 10-15 m2/W and 10-9 esu, respectively, which are about three orders larger than that of NNDC/1,2-dichloroethane solution. Some necessary analyses were presented. The results show that this material is a promising candidate for application in the nonlinear optical devices at 532 nm.

  2. Wavefront sensorless adaptive optics OCT with the DONE algorithm forin vivohuman retinal imaging [Invited].

    Science.gov (United States)

    Verstraete, Hans R G W; Heisler, Morgan; Ju, Myeong Jin; Wahl, Daniel; Bliek, Laurens; Kalkman, Jeroen; Bonora, Stefano; Jian, Yifan; Verhaegen, Michel; Sarunic, Marinko V

    2017-04-01

    In this report, which is an international collaboration of OCT, adaptive optics, and control research, we demonstrate the Data-based Online Nonlinear Extremum-seeker (DONE) algorithm to guide the image based optimization for wavefront sensorless adaptive optics (WFSL-AO) OCT for in vivo human retinal imaging. The ocular aberrations were corrected using a multi-actuator adaptive lens after linearization of the hysteresis in the piezoelectric actuators. The DONE algorithm succeeded in drastically improving image quality and the OCT signal intensity, up to a factor seven, while achieving a computational time of 1 ms per iteration, making it applicable for many high speed applications. We demonstrate the correction of five aberrations using 70 iterations of the DONE algorithm performed over 2.8 s of continuous volumetric OCT acquisition. Data acquired from an imaging phantom and in vivo from human research volunteers are presented.

  3. Adaptive oriented PDEs filtering methods based on new controlling speed function for discontinuous optical fringe patterns

    Science.gov (United States)

    Zhou, Qiuling; Tang, Chen; Li, Biyuan; Wang, Linlin; Lei, Zhenkun; Tang, Shuwei

    2018-01-01

    The filtering of discontinuous optical fringe patterns is a challenging problem faced in this area. This paper is concerned with oriented partial differential equations (OPDEs)-based image filtering methods for discontinuous optical fringe patterns. We redefine a new controlling speed function to depend on the orientation coherence. The orientation coherence can be used to distinguish the continuous regions and the discontinuous regions, and can be calculated by utilizing fringe orientation. We introduce the new controlling speed function to the previous OPDEs and propose adaptive OPDEs filtering models. According to our proposed adaptive OPDEs filtering models, the filtering in the continuous and discontinuous regions can be selectively carried out. We demonstrate the performance of the proposed adaptive OPDEs via application to the simulated and experimental fringe patterns, and compare our methods with the previous OPDEs.

  4. Evaluation of baseline structural factors for predicting glaucomatous visual-field progression using optical coherence tomography, scanning laser polarimetry and confocal scanning laser ophthalmoscopy.

    Science.gov (United States)

    Sehi, M; Bhardwaj, N; Chung, Y S; Greenfield, D S

    2012-12-01

    The objective of this study is to assess whether baseline optic nerve head (ONH) topography and retinal nerve fiber layer thickness (RNFLT) are predictive of glaucomatous visual-field progression in glaucoma suspect (GS) and glaucomatous eyes, and to calculate the level of risk associated with each of these parameters. Participants with ≥28 months of follow-up were recruited from the longitudinal Advanced Imaging for Glaucoma Study. All eyes underwent standard automated perimetry (SAP), confocal scanning laser ophthalmoscopy (CSLO), time-domain optical coherence tomography (TDOCT), and scanning laser polarimetry using enhanced corneal compensation (SLPECC) every 6 months. Visual-field progression was assessed using pointwise linear-regression analysis of SAP sensitivity values (progressor) and defined as significant sensitivity loss of >1 dB/year at ≥2 adjacent test locations in the same hemifield at P<0.01. Cox proportional hazard ratios (HR) were calculated to determine the predictive ability of baseline ONH and RNFL parameters for SAP progression using univariate and multivariate models. Seventy-three eyes of 73 patients (43 GS and 30 glaucoma, mean age 63.2±9.5 years) were enrolled (mean follow-up 51.5±11.3 months). Four of 43 GS (9.3%) and 6 of 30 (20%) glaucomatous eyes demonstrated progression. Mean time to progression was 50.8±11.4 months. Using multivariate models, abnormal CSLO temporal-inferior Moorfields classification (HR=3.76, 95% confidence interval (CI): 1.02-6.80, P=0.04), SLPECC inferior RNFLT (per -1 μm, HR=1.38, 95% CI: 1.02-2.2, P=0.02), and TDOCT inferior RNFLT (per -1 μm, HR=1.11, 95% CI: 1.04-1.2, P=0.001) had significant HRs for SAP progression. Abnormal baseline ONH topography and reduced inferior RNFL are predictive of SAP progression in GS and glaucomatous eyes.

  5. Adapted methods for scanning electron microscopy (SEM in assessment of human sperm morphology

    Directory of Open Access Journals (Sweden)

    Petra Nussdorfer

    2017-07-01

    Full Text Available Infertility is a widespread problem, and in some cases, the routine basic semen analysis is not sufficient to detect the cause of male infertility. The use of the scanning electron microscope (SEM could provide a detailed insight into spermatozoa morphology, but it requires specific sample preparation techniques. The purpose of this study was to select, adjust, and optimize a method for the preparation of spermatozoa samples prior to SEM analysis, and to establish the protocol required for its use in clinical practice. We examined sperm samples of 50 men. The samples were fixed with modified iso-osmolar aldehyde solution followed by osmium post-fixation. In the first method, dehydration of the cells and subsequent critical point drying (CPD were performed on a coverslip. In the second method, the samples were dehydrated in centrifuge tubes; hexamethyldisilazane (HMDS was used as a drying agent instead of CPD, and the samples were air-dried. The third procedure was based on a membrane filter. The samples were dehydrated and dried with HMDS in a Gooch crucible, continuously, without centrifugation or redispersion of the sample. Our results showed that the fixation with modified iso-osmolar aldehyde solution followed by osmium post-fixation, and combined with dehydration and CPD on a coverslip, is the most convenient procedure for SEM sample preparation. In the case of small-size samples or low sperm concentration, dehydration and drying with HMDS on the membrane filter enabled the best reliability, repeatability, and comparability of the results. The presented procedures are suitable for routine use, and they can be applied to confirm as well as to correct a diagnosis.

  6. Replica calibration artefacts for optical 3D scanning of micro parts

    DEFF Research Database (Denmark)

    De Chiffre, Leonardo; Carmignato, S.; Cantatore, Angela

    2009-01-01

    This work deals with development of calibration artefacts produced by using hard replica materials, achieving high quality geometrical reproduction of suitable reference artefacts, high stability, and high surface cooperativeness. An investigation was carried out using a replica material for dental...... applications to reproduce the geometry of a step artefact, a miniature step gauge, and a curve standard for optical measuring machines. The replica artefacts were calibrated using a tactile coordinate measuring machine and measured on two different optical scanners. Replication quality and applicability...

  7. Recording and reproduction of microwave holograms using a scanning procedure and their subsequent optical processing

    Science.gov (United States)

    Hetsch, J.

    1983-01-01

    Intensity distributions in nonoptical wave fields can be visualized and stored on photosensitive material. In the case of microwaves, temperature effects can be utilized with the aid of liquid crystals to visualize intensity distributions. Particular advantages for the study of intensity distributions in microwave fields presents a scanning procedure in which a microcomputer is employed for the control of a probe and the storage of the measured data. The present investigation is concerned with the employment of such a scanning procedure for the recording and the reproduction of microwave holograms. The scanning procedure makes use of an approach discussed by Farhat, et al. (1973). An eight-bit microprocessor with 64 kBytes of RAM is employed together with a diskette storage system.

  8. Robust optical flow using adaptive Lorentzian filter for image reconstruction under noisy condition

    Science.gov (United States)

    Kesrarat, Darun; Patanavijit, Vorapoj

    2017-02-01

    In optical flow for motion allocation, the efficient result in Motion Vector (MV) is an important issue. Several noisy conditions may cause the unreliable result in optical flow algorithms. We discover that many classical optical flows algorithms perform better result under noisy condition when combined with modern optimized model. This paper introduces effective robust models of optical flow by using Robust high reliability spatial based optical flow algorithms using the adaptive Lorentzian norm influence function in computation on simple spatial temporal optical flows algorithm. Experiment on our proposed models confirm better noise tolerance in optical flow's MV under noisy condition when they are applied over simple spatial temporal optical flow algorithms as a filtering model in simple frame-to-frame correlation technique. We illustrate the performance of our models by performing an experiment on several typical sequences with differences in movement speed of foreground and background where the experiment sequences are contaminated by the additive white Gaussian noise (AWGN) at different noise decibels (dB). This paper shows very high effectiveness of noise tolerance models that they are indicated by peak signal to noise ratio (PSNR).

  9. New scanning technique using Adaptive Statistical Iterative Reconstruction (ASIR) significantly reduced the radiation dose of cardiac CT.

    Science.gov (United States)

    Tumur, Odgerel; Soon, Kean; Brown, Fraser; Mykytowycz, Marcus

    2013-06-01

    The aims of our study were to evaluate the effect of application of Adaptive Statistical Iterative Reconstruction (ASIR) algorithm on the radiation dose of coronary computed tomography angiography (CCTA) and its effects on image quality of CCTA and to evaluate the effects of various patient and CT scanning factors on the radiation dose of CCTA. This was a retrospective study that included 347 consecutive patients who underwent CCTA at a tertiary university teaching hospital between 1 July 2009 and 20 September 2011. Analysis was performed comparing patient demographics, scan characteristics, radiation dose and image quality in two groups of patients in whom conventional Filtered Back Projection (FBP) or ASIR was used for image reconstruction. There were 238 patients in the FBP group and 109 patients in the ASIR group. There was no difference between the groups in the use of prospective gating, scan length or tube voltage. In ASIR group, significantly lower tube current was used compared with FBP group, 550 mA (450-600) vs. 650 mA (500-711.25) (median (interquartile range)), respectively, P ASIR group compared with FBP group, 4.29 mSv (2.84-6.02) vs. 5.84 mSv (3.88-8.39) (median (interquartile range)), respectively, P ASIR was associated with increased image noise compared with FBP (39.93 ± 10.22 vs. 37.63 ± 18.79 (mean ± standard deviation), respectively, P ASIR reduces the radiation dose of CCTA without affecting the image quality. © 2013 The Authors. Journal of Medical Imaging and Radiation Oncology © 2013 The Royal Australian and New Zealand College of Radiologists.

  10. A simple but precise method for quantitative measurement of the quality of the laser focus in a scanning optical microscope.

    Science.gov (United States)

    Trägårdh, J; Macrae, K; Travis, C; Amor, R; Norris, G; Wilson, S H; Oppo, G-L; McConnell, G

    2015-07-01

    We report a method for characterizing the focussing laser beam exiting the objective in a laser scanning microscope. This method provides the size of the optical focus, the divergence of the beam, the ellipticity and the astigmatism. We use a microscopic-scale knife edge in the form of a simple transmission electron microscopy grid attached to a glass microscope slide, and a light-collecting optical fibre and photodiode underneath the specimen. By scanning the laser spot from a reflective to a transmitting part of the grid, a beam profile in the form of an error function can be obtained and by repeating this with the knife edge at different axial positions relative to the beam waist, the divergence and astigmatism of the postobjective laser beam can be obtained. The measured divergence can be used to quantify how much of the full numerical aperture of the lens is used in practice. We present data of the beam radius, beam divergence, ellipticity and astigmatism obtained with low (0.15, 0.7) and high (1.3) numerical aperture lenses and lasers commonly used in confocal and multiphoton laser scanning microscopy. Our knife-edge method has several advantages over alternative knife-edge methods used in microscopy including that the knife edge is easy to prepare, that the beam can be characterized also directly under a cover slip, as necessary to reduce spherical aberrations for objectives designed to be used with a cover slip, and it is suitable for use with commercial laser scanning microscopes where access to the laser beam can be limited. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

  11. A Proposal to Localize Fermi GBM GRBs Through Coordinated Scanning of the GBM Error Circle via Optical Telescopes

    Science.gov (United States)

    Ukwatta, T. N.; Linnemann, J. T.; Tollefson, K.; Abeysekara, A. U.; Bhat, P. N.; Sonbas, E.; Gehrels, N.

    2011-01-01

    We investigate the feasibility of implementing a system that will coordinate ground-based optical telescopes to cover the Fermi GBM Error Circle (EC). The aim of the system is to localize GBM detected GRBs and facilitate multi-wavelength follow-up from space and ground. This system will optimize the observing locations in the GBM EC based on individual telescope location, Field of View (FoV) and sensitivity. The proposed system will coordinate GBM EC scanning by professional as well as amateur astronomers around the world. The results of a Monte Carlo simulation to investigate the feasibility of the project are presented.

  12. Converting optical scanning holograms of real objects to binary Fourier holograms using an iterative direct binary search algorithm.

    Science.gov (United States)

    Leportier, Thibault; Park, Min Chul; Kim, You Seok; Kim, Taegeun

    2015-02-09

    In this paper, we present a three-dimensional holographic imaging system. The proposed approach records a complex hologram of a real object using optical scanning holography, converts the complex form to binary data, and then reconstructs the recorded hologram using a spatial light modulator (SLM). The conversion from the recorded hologram to a binary hologram is achieved using a direct binary search algorithm. We present experimental results that verify the efficacy of our approach. To the best of our knowledge, this is the first time that a hologram of a real object has been reconstructed using a binary SLM.

  13. Nano-scale imaging of chromosomes and DNA by scanning near-field optical/atomic force microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Yoshino, Tomoyuki; Sugiyama, Shigeru; Hagiwara, Shoji; Fukushi, Daisuke; Shichiri, Motoharu; Nakao, Hidenobu; Kim, J.-M.; Hirose, Tamaki; Muramatsu, Hiroshi; Ohtani, Toshio

    2003-10-15

    Nano-scale structures of the YOYO-1-stained barley chromosomes and lambda-phage DNA were investigated by scanning near-field optical/atomic force microscopy (SNOM/AFM). This technique enabled precise analysis of fluorescence structural images in relation to the morphology of the biomaterials. The results suggested that the fluorescence intensity does not always correspond to topographic height of the chromosomes, but roughly reflects the local amount and/or density of DNA. Various sizes of the bright fluorescence spots were clearly observed in fluorescence banding-treated chromosomes. Furthermore, fluorescence-stained lambda-phage DNA analysis by SNOM/AFM demonstrated the possibility of nanometer-scale imaging for a novel technique termed nano-fluorescence in situ hybridization (nano-FISH). Thus, SNOM/AFM is a powerful tool for analyzing the structure and the function of biomaterials with higher resolution than conventional optical microscopes.

  14. Colourless adaptively modulated optical OFDM transmitters using SOAs as intensity modulators.

    Science.gov (United States)

    Wei, J L; Yang, X L; Giddings, R P; Tang, J M

    2009-05-25

    The wavelength dependent transmission performance of adaptively modulated optical OFDM (AMOOFDM) signals is investigated, for the first time, over optical amplification- and chromatic dispersion compensation-free IMDD SMF systems using semiconductor optical amplifiers (SOAs) as intensity modulators. A theoretical SOA model describing both optical gain saturation and gain spectral dynamics is developed, based on which optimum SOA operating conditions are identified for various wavelengths varying in a broad range of 1510 nm- 1590 nm. Results show that, SOA intensity modulators operating at the identified optimum conditions enable the realization of colourless AMOOFDM transmitters within the aforementioned wavelength window. Such transmitters are capable of supporting >30 Gb/s signal transmission over 60 km SMFs.

  15. Optical nonlinearity of organic dyes as studied by Z-scan and ...

    Indian Academy of Sciences (India)

    Unknown

    - amino-3,3′-diethyl-10,12-ethylinethiatricarbocyanine perchlorate (IR140) have been measured by using a single beam transmission technique. Z-scan experiments have been used to find out a few nonlinear parameters. The excited state ...

  16. Optical nonlinearity of organic dyes as studied by Z-scan and ...

    Indian Academy of Sciences (India)

    ,3′-diethyl-10,12-ethylinethiatricarbocyanine perchlorate (IR140) have been measured by using a single beam transmission technique. Z-scan experiments have been used to find out a few nonlinear parameters. The excited state relaxation ...

  17. Reducing scan angle using adaptive prior knowledge for a limited-angle intrafraction verification (LIVE) system for conformal arc radiotherapy

    Science.gov (United States)

    Zhang, Yawei; Yin, Fang-Fang; Zhang, You; Ren, Lei

    2017-05-01

    The purpose of this study is to develop an adaptive prior knowledge guided image estimation technique to reduce the scan angle needed in the limited-angle intrafraction verification (LIVE) system for 4D-CBCT reconstruction. The LIVE system has been previously developed to reconstruct 4D volumetric images on-the-fly during arc treatment for intrafraction target verification and dose calculation. In this study, we developed an adaptive constrained free-form deformation reconstruction technique in LIVE to further reduce the scanning angle needed to reconstruct the 4D-CBCT images for faster intrafraction verification. This technique uses free form deformation with energy minimization to deform prior images to estimate 4D-CBCT based on kV-MV projections acquired in extremely limited angle (orthogonal 3°) during the treatment. Note that the prior images are adaptively updated using the latest CBCT images reconstructed by LIVE during treatment to utilize the continuity of the respiratory motion. The 4D digital extended-cardiac-torso (XCAT) phantom and a CIRS 008A dynamic thoracic phantom were used to evaluate the effectiveness of this technique. The reconstruction accuracy of the technique was evaluated by calculating both the center-of-mass-shift (COMS) and 3D volume-percentage-difference (VPD) of the tumor in reconstructed images and the true on-board images. The performance of the technique was also assessed with varied breathing signals against scanning angle, lesion size, lesion location, projection sampling interval, and scanning direction. In the XCAT study, using orthogonal-view of 3° kV and portal MV projections, this technique achieved an average tumor COMS/VPD of 0.4  ±  0.1 mm/5.5  ±  2.2%, 0.6  ±  0.3 mm/7.2  ±  2.8%, 0.5  ±  0.2 mm/7.1  ±  2.6%, 0.6  ±  0.2 mm/8.3  ±  2.4%, for baseline drift, amplitude variation, phase shift, and patient breathing signal variation

  18. Remote optical sensing on the nanometer scale with a bowtie aperture nano-antenna on a fiber tip of scanning near-field optical microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Atie, Elie M.; Xie, Zhihua; El Eter, Ali; Salut, Roland; Baida, Fadi I.; Grosjean, Thierry, E-mail: thierry.grosjean@univ-fcomte.fr [Institut FEMTO-ST, UMR CNRS 6174, Université de Franche-Comté, Département d' Optique P.M. Duffieux, 15B avenue des Montboucons, 25030 Besançon cedex (France); Nedeljkovic, Dusan [Lovalite s.a.s., 7 rue Xavier Marmier, 25000 Besançon (France); Tannous, Tony [Department of Physics, University of Balamand, P.O. Box 100 Tripoli (Lebanon)

    2015-04-13

    Plasmonic nano-antennas have proven the outstanding ability of sensing chemical and physical processes down to the nanometer scale. Sensing is usually achieved within the highly confined optical fields generated resonantly by the nano-antennas, i.e., in contact to the nanostructures. In this paper, we demonstrate the sensing capability of nano-antennas to their larger scale environment, well beyond their plasmonic confinement volume, leading to the concept of “remote” (non contact) sensing on the nanometer scale. On the basis of a bowtie-aperture nano-antenna (BNA) integrated at the apex of a SNOM (Scanning Near-field Optical Microscopy) fiber tip, we introduce an ultra-compact, moveable, and background-free optical nanosensor for the remote sensing of a silicon surface (up to distance of 300 nm). Sensitivity of the BNA to its large scale environment is high enough to expect the monitoring and control of the spacing between the nano-antenna and a silicon surface with sub-nanometer accuracy. This work paves the way towards an alternative class of nanopositioning techniques, based on the monitoring of diffraction-free plasmon resonance, that are alternative to nanomechanical and diffraction-limited optical interference-based devices.

  19. Real time optimization algorithm for wavefront sensorless adaptive optics OCT (Conference Presentation)

    Science.gov (United States)

    Verstraete, Hans R. G. W.; Heisler, Morgan; Ju, Myeong Jin; Wahl, Daniel J.; Bliek, Laurens; Kalkman, Jeroen; Bonora, Stefano; Sarunic, Marinko V.; Verhaegen, Michel; Jian, Yifan

    2017-02-01

    Optical Coherence Tomography (OCT) has revolutionized modern ophthalmology, providing depth resolved images of the retinal layers in a system that is suited to a clinical environment. A limitation of the performance and utilization of the OCT systems has been the lateral resolution. Through the combination of wavefront sensorless adaptive optics with dual variable optical elements, we present a compact lens based OCT system that is capable of imaging the photoreceptor mosaic. We utilized a commercially available variable focal length lens to correct for a wide range of defocus commonly found in patient eyes, and a multi-actuator adaptive lens after linearization of the hysteresis in the piezoelectric actuators for aberration correction to obtain near diffraction limited imaging at the retina. A parallel processing computational platform permitted real-time image acquisition and display. The Data-based Online Nonlinear Extremum seeker (DONE) algorithm was used for real time optimization of the wavefront sensorless adaptive optics OCT, and the performance was compared with a coordinate search algorithm. Cross sectional images of the retinal layers and en face images of the cone photoreceptor mosaic acquired in vivo from research volunteers before and after WSAO optimization are presented. Applying the DONE algorithm in vivo for wavefront sensorless AO-OCT demonstrates that the DONE algorithm succeeds in drastically improving the signal while achieving a computational time of 1 ms per iteration, making it applicable for high speed real time applications.

  20. Data-based online nonlinear extremum-seeker for wavefront sensorless adaptive optics OCT (Conference Presentation)

    Science.gov (United States)

    Jian, Yifan; Verstraete, Hans R. G. W.; Heisler, Morgan; Ju, Myeong Jin; Wahl, Daniel J.; Bliek, Laurens; Kalkman, Jeroen; Bonora, Stefano; Verhaegen, Michel; Sarunic, Marinko V.

    2017-02-01

    Adaptive optics has been successfully applied to cellular resolution imaging of the retina, enabling visualization of the characteristic mosaic patterns of the outer retina. Wavefront sensorless adaptive optics (WSAO) is a novel technique that facilitates high resolution ophthalmic imaging; it replaces the Hartmann-Shack Wavefront Sensor with an image-driven optimization algorithm and mitigates some the challenges encountered with sensor-based designs. However, WSAO generally requires longer time to perform aberrations correction than the conventional closed-loop adaptive optics. When used for in vivo retinal imaging applications, motion artifacts during the WSAO optimization process will affect the quality of the aberration correction. A faster converging optimization scheme needs to be developed to account for rapid temporal variation of the wavefront and continuously apply corrections. In this project, we investigate the Databased Online Nonlinear Extremum-seeker (DONE), a novel non-linear multivariate optimization algorithm in combination with in vivo human WSAO OCT imaging. We also report both hardware and software updates of our compact lens based WSAO 1060nm swept source OCT human retinal imaging system, including real time retinal layer segmentation and tracking (ILM and RPE), hysteresis correction for the multi-actuator adaptive lens, precise synchronization control for the 200kHz laser source, and a zoom lens unit for rapid switching of the field of view. Cross sectional images of the retinal layers and en face images of the cone photoreceptor mosaic acquired in vivo from research volunteers before and after WSAO optimization are presented.

  1. Optic Flow Dominates Visual Scene Polarity in Causing Adaptive Modification of Locomotor Trajectory

    Science.gov (United States)

    Nomura, Y.; Mulavara, A. P.; Richards, J. T.; Brady, R.; Bloomberg, J. J.

    2005-01-01

    Locomotion and posture are influenced and controlled by vestibular, visual and somatosensory information. Optic flow and scene polarity are two characteristics of a visual scene that have been identified as being critical in how they affect perceived body orientation and self-motion. The goal of this study was to determine the role of optic flow and visual scene polarity on adaptive modification in locomotor trajectory. Two computer-generated virtual reality scenes were shown to subjects during 20 minutes of treadmill walking. One scene was a highly polarized scene while the other was composed of objects displayed in a non-polarized fashion. Both virtual scenes depicted constant rate self-motion equivalent to walking counterclockwise around the perimeter of a room. Subjects performed Stepping Tests blindfolded before and after scene exposure to assess adaptive changes in locomotor trajectory. Subjects showed a significant difference in heading direction, between pre and post adaptation stepping tests, when exposed to either scene during treadmill walking. However, there was no significant difference in the subjects heading direction between the two visual scene polarity conditions. Therefore, it was inferred from these data that optic flow has a greater role than visual polarity in influencing adaptive locomotor function.

  2. Traceability of Height Measurements on Green Sand Molds using Optical 3D Scanning

    DEFF Research Database (Denmark)

    Mohaghegh, Kamran; Yazdanbakhsh, S.A.; Tiedje, N. S.

    2016-01-01

    Establishing a reliable measurement procedure for dimensional measurements on green sand molds is a prerequisite for analysis of geometric deviations in mass production of quality castings. Surface of the green sand mold is not suitable for measurements using a tactile coordinate measuring machine....... This paper presents a metrological approach for height measurement on green sand molds using an optical 3D scanner with fringe projection. A new sand sample was developed with a hard binder to withstand the contact force of a touch probe, while keeping optical cooperativeness similar to green sand...

  3. Two cascaded SOAs used as intensity modulators for adaptively modulated optical OFDM signals in optical access networks.

    Science.gov (United States)

    Hamié, Ali; Hamzé, Mohamad; Taki, Haidar; Makouk, Layaly; Sharaiha, Ammar; Alaeddine, Ali; Al Housseini, Ali; Giacoumidis, Elias; Tang, J M

    2014-06-30

    Detailed theoretical and numerical investigations of the transmission performance of adaptively modulated optical orthogonal frequency division multiplexed (AMOOFDM) signals are undertaken, for the first time, in optical amplification and chromatic dispersion (CD) compensation free single mode fiber (SMF) intensity-modulated and direct-detection (IMDD) systems using two cascaded semiconductor optical amplifiers in a counterpropagating configuration as an intensity modulator (TC-SOA-CC-IM). A theoretical model describing the characteristics of this configuration is developed. Extensive performance comparisons are also made between the TC-SOA-CC and the single SOA intensity modulators. It is shown that, the TC-SOA-CC reaches its strongly saturated region using a lower input optical power much faster than the single SOA resulting in significantly reduced effective carrier lifetime and thus wide TC-SOA-CC bandwidths. It is shown that at low input optical power, we can increase the signal line rate almost 115% which will be more than twice the transmission performance offered by single SOA. In addition, the TC-SOA-CC-IM is capable of supporting signal line rates higher than corresponding to the SOA-IM by using 10dB lower input optical powers. For long transmission distance, the TC-SOA-CC-IM has much stronger CD compensation capability compared to the SOA-IM. In addition the use of TC-SOA-CC-IM is more effective regarding the capability to benefit from the CD compensation for shorter distances starting at 60km SMF, whilst for the SOA-IM starting at 90km.

  4. Z-scan and optical limiting properties of Hibiscus sabdariffa dye

    CSIR Research Space (South Africa)

    Maaza, M

    2014-12-01

    Full Text Available ). 2. C. Pradeep, S. Mathew, B. Nithyajam P. Radhakrishnan and V.P.N. Nampoori, Appl Phys A: Materials Science & Processing, DOI 10.1007/s00339-013-7814-0 (2013). 3. F. Z. Henari and A. Al-Saie, Nonlinear and Quantum Optics-Laser Physics, Vol. 16, No...

  5. Noninvasive monitoring of glucose concentration using differential absorption low-coherence interferometry based on rapid scanning optical delay line

    Energy Technology Data Exchange (ETDEWEB)

    Zhou Yong; Zeng Nan; He Yonghong, E-mail: heyh@sz.tsinghua.edu.cn [Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, 518055 (China)

    2011-01-01

    A non-invasive method of detecting glucose concentration using differential absorption low-coherence interferometry (DALCI) based on rapid scanning optical delay line is presented. Two light sources, one centered within (1625 nm) a glucose absorption band, while the other outside (1310 nm) the glucose absorption band, are used in the experiment. The low-coherence interferometry (LCI) is employed to obtain the signals back-reflecting from the iris which carries the messages of material concentration in anterior chamber. Using rapid scanning optical delay line (RSOD) as the reference arm, we can detect the signals in a very short time. Therefore the glucose concentration can be monitored in real-time, which is very important for the detection in vivo. In our experiments, the cornea and aqueous humor can be treated as nearly non-scattering substance. The difference in the absorption coefficient is much larger than the difference in the scattering coefficient, so the influence of scattering can be neglected. By subtracting the algorithmic low-coherence interference signals of the two wavelengths, the absorption coefficient can be calculated which is proportional to glucose concentration. To reduce the speckle noise, a 30 variation of signals were used before the final calculation of the glucose concentration. The improvements of our experiment are also discussed in the article. The method has a potential application for noninvasive detection of glucose concentration in vivo and in real-time.

  6. Fuzzy-Based Adaptive Hybrid Burst Assembly Technique for Optical Burst Switched Networks

    Directory of Open Access Journals (Sweden)

    Abubakar Muhammad Umaru

    2014-01-01

    Full Text Available The optical burst switching (OBS paradigm is perceived as an intermediate switching technology for future all-optical networks. Burst assembly that is the first process in OBS is the focus of this paper. In this paper, an intelligent hybrid burst assembly algorithm that is based on fuzzy logic is proposed. The new algorithm is evaluated against the traditional hybrid burst assembly algorithm and the fuzzy adaptive threshold (FAT burst assembly algorithm via simulation. Simulation results show that the proposed algorithm outperforms the hybrid and the FAT algorithms in terms of burst end-to-end delay, packet end-to-end delay, and packet loss ratio.

  7. Adaptive Bit Rate Video Streaming Through an RF/Free Space Optical Laser Link

    Directory of Open Access Journals (Sweden)

    A. Akbulut

    2010-06-01

    Full Text Available This paper presents a channel-adaptive video streaming scheme which adjusts video bit rate according to channel conditions and transmits video through a hybrid RF/free space optical (FSO laser communication system. The design criteria of the FSO link for video transmission to 2.9 km distance have been given and adaptive bit rate video streaming according to the varying channel state over this link has been studied. It has been shown that the proposed structure is suitable for uninterrupted transmission of videos over the hybrid wireless network with reduced packet delays and losses even when the received power is decreased due to weather conditions.

  8. Effect of the menstrual cycle on the optic nerve head in diabetes: analysis by confocal scanning laser ophthalmoscopy.

    Science.gov (United States)

    Akar, Munire Erman; Yucel, Iclal; Erdem, Uzeyir; Taskin, Omur; Ozel, Alper; Akar, Yusuf

    2005-04-01

    The purpose of this study was to examine and compare menstrual-cycle-dependent topographic changes in the optic nerve head of normally menstruating women with different grades of type 2 diabetes mellitus. We studied the right eyes of 123 normally menstruating women (36 with severe nonproliferative diabetic retinopathy [NPDR], 42 with mild NPDR and 45 healthy subjects). All subjects underwent a complete ocular examination at baseline. At 4 hormonally distinct phases of the menstrual cycle (early follicular, late follicular, mid-luteal and late luteal), we analysed the topography of the optic nerve head, using a confocal scanning laser ophthalmoscope, and measured the serum levels of estradiol, progesterone and luteinizing hormone. We excluded from analysis the data for 8 patients with severe NPDR, 10 patients with mild NPDR and 15 control subjects who were lost to follow-up examinations during the menstrual cycle. The mean age and optic disc area did not differ significantly among the 3 groups. The duration of diabetes was significantly longer in the patients with severe NPDR than in those with mild NPDR (p cup-shape measure, linear cup/disc ratio, cup/disc area ratio and cup area in the late luteal phase compared with the other phases of the menstrual cycle (p menstrual cycle. Severe NPDR is associated with significant topographic changes in the rim and cup of the optic nerve head during the menstrual cycle. This must be considered in the evaluation of women with both diabetes and glaucoma. The normal fluctuations in serum sex hormone levels during the menstrual cycle of diabetic women seem to affect the optic nerve head more when the disease is advanced.

  9. Development of electron optical system using annular pupils for scanning transmission electron microscope by focused ion beam

    Energy Technology Data Exchange (ETDEWEB)

    Matsutani, Takaomi, E-mail: matutani@ele.kindai.ac.jp [Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502 (Japan); Yasumoto, Tsuchika; Tanaka, Takeo [Osaka Sangyo University, 3-1-1 Nakagaito, Daito, Osaka 574-8530 (Japan); Kawasaki, Tadahiro; Ichihashi, Mikio [Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603 (Japan); Ikuta, Takashi [Osaka Electro-Communication University, 18-8 Hatsu-cho, Neyagawa, Osaka 572-8530 (Japan)

    2012-02-01

    Annular pupils for electron optics were produced using a focused ion beam (FIB), enabling an increase in the depth of focus and allowing for aberration-free imaging and separation of the amplitude and phase images in a scanning transmission electron microscope (STEM). Simulations demonstrate that an increased focal depth is advantageous for three-dimensional tomography in the STEM. For a 200 kV electron beam, the focal depth is increased to approximately 100 nm by using an annular pupil with inner and outer semi-angles of 29 and 30 mrad, respectively. Annular pupils were designed with various outer diameters of 40-120 {mu}m and the inner diameter was designed at 80% of the outer diameter. A taper angle varying from 1 Degree-Sign to 20 Degree-Sign was applied to the slits of the annular pupils to suppress the influence of high-energy electron scattering. The fabricated annular pupils were inspected by scanning ion beam microscopy and scanning electron microscopy. These annular pupils were loaded into a STEM and no charge-up effects were observed in the scintillator projection images recorded by a CCD camera.

  10. Analysis of the Origin of Atypical Scanning Laser Polarimetry Patterns by Polarization-Sensitive Optical Coherence Tomography

    Science.gov (United States)

    Götzinger, Erich; Pircher, Michael; Baumann, Bernhard; Hirn, Cornelia; Vass, Clemens; Hitzenberger, Christoph K.

    2010-01-01

    Purpose To analyze the physical origin of atypical scanning laser polarimetry (SLP) patterns. To compare polarization-sensitive optical coherence tomography (PS-OCT) scans to SLP images. To present a method to obtain pseudo-SLP images by PS-OCT that are free of atypical artifacts. Methods Forty-one eyes of healthy subjects, subjects with suspected glaucoma, and patients with glaucoma were imaged by SLP (GDx VCC) and a prototype spectral domain PS-OCT system. The PS-OCT system acquires three-dimensional (3D) datasets of intensity, retardation, and optic axis orientation simultaneously within 3 seconds. B-scans of intensity and retardation and en face maps of retinal nerve fiber layer (RNFL) retardation were derived from the 3D PS-OCT datasets. Results were compared with those obtained by SLP. Results Twenty-two eyes showed atypical retardation patterns, and 19 eyes showed normal patterns. From the 22 atypical eyes, 15 showed atypical patterns in both imaging modalities, five were atypical only in SLP images, and two were atypical only in PS-OCT images. In most (15 of 22) atypical cases, an increased penetration of the probing beam into the birefringent sclera was identified as the source of atypical patterns. In such cases, the artifacts could be eliminated in PS-OCT images by depth segmentation and exclusion of scleral signals. Conclusions PS-OCT provides deeper insight into the contribution of different fundus layers to SLP images. Increased light penetration into the sclera can distort SLP retardation patterns of the RNFL. PMID:19036999

  11. Measurement of retinal nerve fiber layer thickness in eyes with optic disc swelling by using scanning laser polarimetry and optical coherence tomography.

    Science.gov (United States)

    Hata, Masayuki; Miyamoto, Kazuaki; Oishi, Akio; Kimura, Yugo; Nakagawa, Satoko; Horii, Takahiro; Yoshimura, Nagahisa

    2014-01-01

    The retinal nerve fiber layer thickness (RNFLT) in patients with optic disc swelling of different etiologies was compared using scanning laser polarimetry (SLP) and spectral-domain optical coherence tomography (OCT). Forty-seven patients with optic disc swelling participated in the cross-sectional study. Both GDx SLP (enhanced corneal compensation) and Spectralis spectral-domain OCT measurements of RNFLT were made in 19 eyes with papilledema (PE), ten eyes with optic neuritis (ON), and 18 eyes with nonarteritic anterior ischemic optic neuropathy (NAION) at the neuro-ophthalmology clinic at Kyoto University Hospital. Differences in SLP (SLP-RNFLT) and OCT (OCT-RNFLT) measurements among different etiologies were investigated. No statistical differences in average OCT-RNFLT among PE, ON, and NAION patients were noted. Average SLP-RNFLT in NAION patients was smaller than in PE (P<0.01) or ON (P=0.02) patients. When RNFLT in each retinal quadrant was compared, no difference among etiologies was noted on OCT, but on SLP, the superior quadrant was thinner in NAION than in PE (P<0.001) or ON (P=0.001) patients. Compared with age-adjusted normative data of SLP-RNFLT, average SLP-RNFLT in PE (P<0.01) and ON (P<0.01) patients was greater. Superior SLP-RNFLT in NAION patients was smaller (P=0.026). The ratio of average SLP-RNFLT to average OCT-RNFLT was smaller in NAION than in PE (P=0.001) patients. In the setting of RNFL thickening, despite increased light retardance in PE and ON eyes, SLP revealed that NAION eyes have less retardance, possibly associated with ischemic axonal loss.

  12. Integration of Optical Coherence Tomography Scan Patterns to Augment Clinical Data Suite

    Science.gov (United States)

    Mason, S.; Patel, N.; Van Baalen, M.; Tarver, W.; Otto, C.; Samuels, B.; Koslovsky, M.; Schaefer, C.; Taiym, W.; Wear, M.; hide

    2018-01-01

    Vision changes identified in long duration spaceflight astronauts has led Space Medicine at NASA to adopt a more comprehensive clinical monitoring protocol. Optical Coherence Tomography (OCT) was recently implemented at NASA, including on board the International Space Station in 2013. NASA is collaborating with Heidelberg Engineering to increase the fidelity of the current OCT data set by integrating the traditional circumpapillary OCT image with radial and horizontal block images at the optic nerve head. The retinal nerve fiber layer was segmented by two experienced individuals. Intra-rater (N=4 subjects and 70 images) and inter-rater (N=4 subjects and 221 images) agreement was performed. The results of this analysis and the potential benefits will be presented.

  13. Differential polarization nonlinear optical microscopy with adaptive optics controlled multiplexed beams.

    Science.gov (United States)

    Samim, Masood; Sandkuijl, Daaf; Tretyakov, Ian; Cisek, Richard; Barzda, Virginijus

    2013-09-09

    Differential polarization nonlinear optical microscopy has the potential to become an indispensable tool for structural investigations of ordered biological assemblies and microcrystalline aggregates. Their microscopic organization can be probed through fast and sensitive measurements of nonlinear optical signal anisotropy, which can be achieved with microscopic spatial resolution by using time-multiplexed pulsed laser beams with perpendicular polarization orientations and photon-counting detection electronics for signal demultiplexing. In addition, deformable membrane mirrors can be used to correct for optical aberrations in the microscope and simultaneously optimize beam overlap using a genetic algorithm. The beam overlap can be achieved with better accuracy than diffraction limited point-spread function, which allows to perform polarization-resolved measurements on the pixel-by-pixel basis. We describe a newly developed differential polarization microscope and present applications of the differential microscopy technique for structural studies of collagen and cellulose. Both, second harmonic generation, and fluorescence-detected nonlinear absorption anisotropy are used in these investigations. It is shown that the orientation and structural properties of the fibers in biological tissue can be deduced and that the orientation of fluorescent molecules (Congo Red), which label the fibers, can be determined. Differential polarization microscopy sidesteps common issues such as photobleaching and sample movement. Due to tens of megahertz alternating polarization of excitation pulses fast data acquisition can be conveniently applied to measure changes in the nonlinear signal anisotropy in dynamically changing in vivo structures.

  14. Pulse front adaptive optics: a new method for control of ultrashort laser pulses.

    Science.gov (United States)

    Sun, Bangshan; Salter, Patrick S; Booth, Martin J

    2015-07-27

    Ultrafast lasers enable a wide range of physics research and the manipulation of short pulses is a critical part of the ultrafast tool kit. Current methods of laser pulse shaping are usually considered separately in either the spatial or the temporal domain, but laser pulses are complex entities existing in four dimensions, so full freedom of manipulation requires advanced forms of spatiotemporal control. We demonstrate through a combination of adaptable diffractive and reflective optical elements - a liquid crystal spatial light modulator (SLM) and a deformable mirror (DM) - decoupled spatial control over the pulse front (temporal group delay) and phase front of an ultra-short pulse was enabled. Pulse front modulation was confirmed through autocorrelation measurements. This new adaptive optics technique, for the first time enabling in principle arbitrary shaping of the pulse front, promises to offer a further level of control for ultrafast lasers.

  15. Optimizing stochastic gradient descent algorithms for serially addressed adaptive-optics wavefront modulators.

    Science.gov (United States)

    Simpkins, Travis; Hui, Jeremy; Warde, Cardinal

    2007-10-20

    High-resolution adaptive-optical systems with thousands to millions of pixels will most likely have to employ serial- or matrix-addressed spatial light modulators (e.g., microelectromechanical-system-on-VLSI spatial light modulators). We compare parallel gradient descent adaptive-optics algorithms with serial gradient descent algorithms running on serially addressed modulators. While serial algorithms have previously been shown to require more iterations than parallel algorithms, we show that, because of the limitations of the databus, each serial iteration of the algorithm on a serial modulator requires significantly less time to complete than a parallel iteration, thereby favoring the serial algorithm when time to convergence is used as the performance metric. Thus, such high-resolution serially addressed devices are generally better matched to the serial-update wavefront correction algorithm owing to the data load penalty imposed by the bandwidth-limited databus of these modulators.

  16. Modelling the multi-conjugate adaptive optics system of the European Extremely Large Telescope

    Science.gov (United States)

    Schreiber, L.; Arcidiacono, C.; Bregoli, G.; Diolaiti, E.; Butler, R. C.; Foppiani, I.; Lombini, M.; Patti, M.; Ciliegi, P.

    MAORY is the Multi-Conjugate Adaptive Optics Module for the E-ELT. The baseline design assumes six sodium Laser Guide Stars and three Natural Guide Stars for wavefront sensing. Three deformable mirrors, including the telescope adaptive mirror M4, are optically conjugated to different altitudes in the atmosphere to achieve compensation of the atmospheric turbulence effects over an extended Field of View. In preparation for the project phase-B we are analyzing different critical aspects of such a system. We are developing a versatile and modular end-to-end simulation code that makes use of GPUs to obtain high-fidelity modelling of the system performance and, in parallel, a semplified code for the analysis of the effects induced by the temporal variation of the sodium layer where the artificial laser guide stars are generated. An overview of the work in progress will be given.

  17. A high sensitivity optically stimulated luminescence scanning system for measurement of single sand-sized grains

    DEFF Research Database (Denmark)

    Duller, G.A.T.; Bøtter-Jensen, L.; Kohsiek, P.

    1999-01-01

    An instrument has been designed for the routine analysis of the optically stimulated luminescence signal from single grains of sand. The system is capable of analysing over 3000 individual grains in a single measurement sequence, and the OSL signal from each grain can be read in less than 3 s....... The design principles are described, along with preliminary measurements that illustrate the operation of the system and its capabilities....

  18. Investigation on a replica step gauge for optical 3D scanning of micro parts

    DEFF Research Database (Denmark)

    Cantatore, Angela; De Chiffre, Leonardo; Carmignato, S.

    2010-01-01

    This work deals with investigation of the stability over time and surface cooperativeness of a calibration artefact intended for optical scanner verification. A replica step gauge with 11 grooves, made of bisacryl material for dental applications (luxabite) and previously fabricated was studied. ...... measurements. Results demonstrate good stability of the step gauge and material transparency good cooperativeness, which is compensated when a unidirectional strategy is followed....

  19. Micrometric precision of prosthetic dental crowns obtained by optical scanning and computer-aided designing/computer-aided manufacturing system

    Science.gov (United States)

    das Neves, Flávio Domingues; de Almeida Prado Naves Carneiro, Thiago; do Prado, Célio Jesus; Prudente, Marcel Santana; Zancopé, Karla; Davi, Letícia Resende; Mendonça, Gustavo; Soares, Carlos José

    2014-08-01

    The current study evaluated prosthetic dental crowns obtained by optical scanning and a computer-aided designing/computer-aided manufacturing system using micro-computed tomography to compare the marginal fit. The virtual models were obtained with four different scanning surfaces: typodont (T), regular impressions (RI), master casts (MC), and powdered master casts (PMC). Five virtual models were obtained for each group. For each model, a crown was designed on the software and milled from feldspathic ceramic blocks. Micro-CT images were obtained for marginal gap measurements and the data were statistically analyzed by one-way analysis of variance followed by Tukey's test. The mean vertical misfit was T=62.6±65.2 μm; MC=60.4±38.4 μm; PMC=58.1±38.0 μm, and RI=89.8±62.8 μm. Considering a percentage of vertical marginal gap of up to 75 μm, the results were T=71.5%, RI=49.2%, MC=69.6%, and PMC=71.2%. The percentages of horizontal overextension were T=8.5%, RI=0%, MC=0.8%, and PMC=3.8%. Based on the results, virtual model acquisition by scanning the typodont (simulated mouth) or MC, with or without powder, showed acceptable values for the marginal gap. The higher result of marginal gap of the RI group suggests that it is preferable to scan this directly from the mouth or from MC.

  20. Clear widens the field for observations of the Sun with multi-conjugate adaptive optics

    Science.gov (United States)

    Schmidt, Dirk; Gorceix, Nicolas; Goode, Philip R.; Marino, Jose; Rimmele, Thomas; Berkefeld, Thomas; Wöger, Friedrich; Zhang, Xianyu; Rigaut, François; von der Lühe, Oskar

    2017-01-01

    The multi-conjugate adaptive optics (MCAO) pathfinder Clear on the New Solar Telescope in Big Bear Lake has provided the first-ever MCAO-corrected observations of the Sun that show a clearly and visibly widened corrected field of view compared to quasi-simultaneous observations with classical adaptive optics (CAO) correction. Clear simultaneously uses three deformable mirrors, each conjugated to a different altitude, to compensate for atmospheric turbulence. While the MCAO correction was most effective over an angle that is approximately three times wider than the angle that was corrected by CAO, the full 53'' field of view did benefit from MCAO correction. We further demonstrate that ground-layer-only correction is attractive for solar observations as a complementary flavor of adaptive optics for observational programs that require homogenous seeing improvement over a wide field rather than diffraction-limited resolution. We show illustrative images of solar granulation and of a sunspot obtained on different days in July 2016, and present a brief quantitative analysis of the generalized Fried parameters of the images. The movies associated to Fig. 1 are available at http://www.aanda.org

  1. High temporal resolution aberrometry in a 50-eye population and implications for adaptive optics error budget.

    Science.gov (United States)

    Jarosz, Jessica; Mecê, Pedro; Conan, Jean-Marc; Petit, Cyril; Paques, Michel; Meimon, Serge

    2017-04-01

    We formed a database gathering the wavefront aberrations of 50 healthy eyes measured with an original custom-built Shack-Hartmann aberrometer at a temporal frequency of 236 Hz, with 22 lenslets across a 7-mm diameter pupil, for a duration of 20 s. With this database, we draw statistics on the spatial and temporal behavior of the dynamic aberrations of the eye. Dynamic aberrations were studied on a 5-mm diameter pupil and on a 3.4 s sequence between blinks. We noted that, on average, temporal wavefront variance exhibits a n -2 power-law with radial order n and temporal spectra follow a f -1.5 power-law with temporal frequency f . From these statistics, we then extract guidelines for designing an adaptive optics system. For instance, we show the residual wavefront error evolution as a function of the number of corrected modes and of the adaptive optics loop frame rate. In particular, we infer that adaptive optics performance rapidly increases with the loop frequency up to 50 Hz, with gain being more limited at higher rates.

  2. Adaptive finite element methods for the solution of inverse problems in optical tomography

    Science.gov (United States)

    Bangerth, Wolfgang; Joshi, Amit

    2008-06-01

    Optical tomography attempts to determine a spatially variable coefficient in the interior of a body from measurements of light fluxes at the boundary. Like in many other applications in biomedical imaging, computing solutions in optical tomography is complicated by the fact that one wants to identify an unknown number of relatively small irregularities in this coefficient at unknown locations, for example corresponding to the presence of tumors. To recover them at the resolution needed in clinical practice, one has to use meshes that, if uniformly fine, would lead to intractably large problems with hundreds of millions of unknowns. Adaptive meshes are therefore an indispensable tool. In this paper, we will describe a framework for the adaptive finite element solution of optical tomography problems. It takes into account all steps starting from the formulation of the problem including constraints on the coefficient, outer Newton-type nonlinear and inner linear iterations, regularization, and in particular the interplay of these algorithms with discretizing the problem on a sequence of adaptively refined meshes. We will demonstrate the efficiency and accuracy of these algorithms on a set of numerical examples of clinical relevance related to locating lymph nodes in tumor diagnosis.

  3. Guide-star-based computational adaptive optics for broadband interferometric tomography

    Science.gov (United States)

    Adie, Steven G.; Shemonski, Nathan D.; Graf, Benedikt W.; Ahmad, Adeel; Scott Carney, P.; Boppart, Stephen A.

    2012-11-01

    We present a method for the numerical correction of optical aberrations based on indirect sensing of the scattered wavefront from point-like scatterers ("guide stars") within a three-dimensional broadband interferometric tomogram. This method enables the correction of high-order monochromatic and chromatic aberrations utilizing guide stars that are revealed after numerical compensation of defocus and low-order aberrations of the optical system. Guide-star-based aberration correction in a silicone phantom with sparse sub-resolution-sized scatterers demonstrates improvement of resolution and signal-to-noise ratio over a large isotome. Results in highly scattering muscle tissue showed improved resolution of fine structure over an extended volume. Guide-star-based computational adaptive optics expands upon the use of image metrics for numerically optimizing the aberration correction in broadband interferometric tomography, and is analogous to phase-conjugation and time-reversal methods for focusing in turbid media.

  4. New adaptive optics control strategy for petawatt-class laser chains

    Science.gov (United States)

    Varkentina, N.; Dovillaire, G.; Legrand, J.; Beaugrand, G.; Stefanon, I.; Treimany, P.; Levecq, X.

    2017-08-01

    A new generation of ultra-high intensity femtosecond petawatt- and above-class lasers requires new approaches to wavefront corrections. New challenges for adaptive optics consist in overcoming the constraints of potentially bigger diameters, larger amplitude aberrations, faster optics, higher risk of damaging optical components and faster and easier maintenance. Here we present a new technology of a mechanical deformable mirror, which has a large stroke, high temporal stability, low hysteresis, no printthrough effect, easy, safe and fast maintenance and an operating frequency up to 10 Hz. We propose the full correction of the final focal spot in the target chamber by a combination of a standard adaptive optics system, a simple focal plane camera and a phase retrieval correction process. We test the reliability of the correction system in terms of intensity variation and wavefront stability. We further verify correction robustness of the method on a large spectral bandwidth and finally perform a focal spot correction on a terawatt laser system in both low and high-power regimes.

  5. Real-time optical tracking for motion compensated irradiation with scanned particle beams at CNAO

    Energy Technology Data Exchange (ETDEWEB)

    Fattori, G., E-mail: giovanni.fattori@psi.ch [Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Seregni, M. [Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Pella, A. [Centro Nazionale di Adroterapia Oncologica (CNAO), Strada Campeggi 53, 27100 Pavia (Italy); Riboldi, M. [Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Capasso, L. [Istituto Nazionale di Fisica Nucleare, Section of Torino, Torino 10125 (Italy); Donetti, M. [Centro Nazionale di Adroterapia Oncologica (CNAO), Strada Campeggi 53, 27100 Pavia (Italy); Istituto Nazionale di Fisica Nucleare, Section of Torino, Torino 10125 (Italy); Ciocca, M. [Centro Nazionale di Adroterapia Oncologica (CNAO), Strada Campeggi 53, 27100 Pavia (Italy); Giordanengo, S. [Istituto Nazionale di Fisica Nucleare, Section of Torino, Torino 10125 (Italy); Pullia, M. [Centro Nazionale di Adroterapia Oncologica (CNAO), Strada Campeggi 53, 27100 Pavia (Italy); Marchetto, F. [Istituto Nazionale di Fisica Nucleare, Section of Torino, Torino 10125 (Italy); Baroni, G. [Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano (Italy); Centro Nazionale di Adroterapia Oncologica (CNAO), Strada Campeggi 53, 27100 Pavia (Italy)

    2016-08-11

    Purpose: We describe the interface developed at the National Center for Oncological Hadrontherapy in Pavia to provide the dose delivery systems with real time respiratory motion information captured with an optical tracking system. An experimental study is presented to assess the technical feasibility of the implemented organ motion compensation framework, by analyzing the film response when irradiated with proton beams. Methods: The motion monitoring solution is based on a commercial hardware for motion capture running in-house developed software for respiratory signal processing. As part of the integration, the latency of data transmission to the dose delivery system was experimentally quantified and accounted for by signal time prediction. A respiratory breathing phantom is presented and used to test tumor tracking based either on the optical measurement of the target position or internal-external correlation models and beam gating, as driven by external surrogates. Beam tracking was tested considering the full target motion excursion (25×18 mm), whereas it is limited to 6×2 mm in the gating window. The different motion mitigation strategies were evaluated by comparing the experimental film responses with respect to static irradiation conditions. Dose inhomogeneity (IC) and conformity (CI) are provided as main indexes for dose quality assessment considering the irradiation in static condition as reference. Results: We measured 20.6 ms overall latency for motion signal processing. Dose measurements showed that beam tracking largely preserved dose homogeneity and conformity, showing maximal IC and CI variations limited to +0.10 and −0.01 with respect to the static reference. Gating resulted in slightly larger discrepancies (ΔIC=+0.20, ΔCI=−0.13) due to uncompensated residual motion in the gating window. Conclusions: The preliminary beam tracking and gating results verified the functionality of the prototypal solution for organ motion compensation based on

  6. Frequency Adaptive Control Technique for Periodic Runout and Wobble Cancellation in Optical Disk Drives

    Directory of Open Access Journals (Sweden)

    Yee-Pien Yang

    2006-10-01

    Full Text Available Periodic disturbance occurs in various applications on the control of the rotational mechanical systems. For optical disk drives, the spirally shaped tracks are usually not perfectly circular and the assembly of the disk and spindle motor is unavoidably eccentric. The resulting periodic disturbance is, therefore, synchronous with the disk rotation, and becomes particularly noticeable for the track following and focusing servo system. This paper applies a novel adaptive controller, namely Frequency Adaptive Control Technique (FACT, for rejecting the periodic runout and wobble effects in the optical disk drive with dual actuators. The control objective is to attenuate adaptively the specific frequency contents of periodic disturbances without amplifying its rest harmonics. FACT is implemented in a plug-in manner and provides a suitable framework for periodic disturbance rejection in the cases where the fundamental frequencies of the disturbance are alterable. It is shown that the convergence property of parameters in the proposed adaptive algorithm is exponentially stable. It is applicable to both the spindle modes of constant linear velocity (CLV and constant angular velocity (CAV for various operation speeds. The experiments showed that the proposed FACT has successful improvement on the tracking and focusing performance of the CD-ROM, and is extended to various compact disk drives.

  7. Clinical Validation of a Smartphone-Based Adapter for Optic Disc Imaging in Kenya.

    Science.gov (United States)

    Bastawrous, Andrew; Giardini, Mario Ettore; Bolster, Nigel M; Peto, Tunde; Shah, Nisha; Livingstone, Iain A T; Weiss, Helen A; Hu, Sen; Rono, Hillary; Kuper, Hannah; Burton, Matthew

    2016-02-01

    Visualization and interpretation of the optic nerve and retina are essential parts of most physical examinations. To design and validate a smartphone-based retinal adapter enabling image capture and remote grading of the retina. This validation study compared the grading of optic nerves from smartphone images with those of a digital retinal camera. Both image sets were independently graded at Moorfields Eye Hospital Reading Centre. Nested within the 6-year follow-up (January 7, 2013, to March 12, 2014) of the Nakuru Eye Disease Cohort in Kenya, 1460 adults (2920 eyes) 55 years and older were recruited consecutively from the study. A subset of 100 optic disc images from both methods were further used to validate a grading app for the optic nerves. Data analysis was performed April 7 to April 12, 2015. Vertical cup-disc ratio for each test was compared in terms of agreement (Bland-Altman and weighted κ) and test-retest variability. A total of 2152 optic nerve images were available from both methods (also 371 from the reference camera but not the smartphone, 170 from the smartphone but not the reference camera, and 227 from neither the reference camera nor the smartphone). Bland-Altman analysis revealed a mean difference of 0.02 (95% CI, -0.21 to 0.17) and a weighted κ coefficient of 0.69 (excellent agreement). The grades of an experienced retinal photographer were compared with those of a lay photographer (no health care experience before the study), and no observable difference in image acquisition quality was found. Nonclinical photographers using the low-cost smartphone adapter were able to acquire optic nerve images at a standard that enabled independent remote grading of the images comparable to those acquired using a desktop retinal camera operated by an ophthalmic assistant. The potential for task shifting and the detection of avoidable causes of blindness in the most at-risk communities makes this an attractive public health intervention.

  8. Dental image replacement on cone beam computed tomography with three-dimensional optical scanning of a dental cast, occlusal bite, or bite tray impression.

    Science.gov (United States)

    Kang, S-H; Lee, J-W; Lim, S-H; Kim, Y-H; Kim, M-K

    2014-10-01

    The goal of the present study was to compare the accuracy of dental image replacement on a cone beam computed tomography (CBCT) image using digital image data from three-dimensional (3D) optical scanning of a dental cast, occlusal bite, and bite tray impression. A Bracket Typodont dental model was used. CBCT of the dental model was performed and the data were converted to stereolithography (STL) format. Three experimental materials, a dental cast, occlusal bite, and bite tray impression, were optically scanned in 3D. STL files converted from the CBCT of the Typodont model and the 3D optical-scanned STL files of the study materials were image-registered. The error range of each methodology was measured and compared with a 3D optical scan of the Typodont. For the three materials, the smallest error observed was 0.099±0.114mm (mean error±standard deviation) for registering the 3D optical scan image of the dental cast onto the CBCT dental image. Although producing a dental cast can be laborious, the study results indicate that it is the preferred method. In addition, an occlusal bite is recommended when bite impression materials are used. Copyright © 2014 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.

  9. First experiences with a slitlamp-adapted optical coherence tomography (OCT) system in the anterior and posterior segment of the eye

    Science.gov (United States)

    Hoerauf, Hans; Scholz, Christian; Engelhardt, Ralf; Koch, Peter; Laqua, Horst; Birngruber, Reginald

    1999-02-01

    Optical Coherence Tomography (OCT) is a new in vivo imaging device in ophthalmology. We investigated the diagnostic potentials of a slitlamp-adapted OCT in the examination of not only the posterior but also the anterior segment in vivo. Patients were examined with a new prototype of a slitlamp- adapted OCT (Fa.Schwind, D-Kleinostheim) in the anterior and posterior segment using 100 - 200 axial scans with 50 Hz scan frequency. The scan length is variable up to 7.5 mm. The software allows to evaluate the images in gray scale or false color. By the slitlamp-adapted OCT all anatomic structures and morphological changes anterior to the attenuating iris pigment epithelium including the anterior chamber angle can be demonstrated and measured precisely. Within the pupillary opening OCT-imaging of cataract, secondary cataract formation as well as the anterior vitreous can be performed. The slitlamp-adapted OCT also allows direct biomicroscopic control of the measured area in examinations of the posterior segment. The slitlamp-adapted OCT provides a new and helpful diagnostic tool, which allows a precise examination of the anterior and posterior segment of the eye. The advantage of this new method lies in the combination of the familiar examination technique and the simultaneous slitlamp view, which provides an exact position control. It is possible to do pachymetric studies of the cornea after photorefractive and phototherapeutic therapy. Of importance is the objective measurement of the chamber angle, which may improve pre- and postoperative evaluation and allow a better follow up documentation of glaucoma patients.

  10. Multimodal swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography at 400 kHz

    Science.gov (United States)

    El-Haddad, Mohamed T.; Joos, Karen M.; Patel, Shriji N.; Tao, Yuankai K.

    2017-02-01

    Multimodal imaging systems that combine scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) have demonstrated the utility of concurrent en face and volumetric imaging for aiming, eye tracking, bulk motion compensation, mosaicking, and contrast enhancement. However, this additional functionality trades off with increased system complexity and cost because both SLO and OCT generally require dedicated light sources, galvanometer scanners, relay and imaging optics, detectors, and control and digitization electronics. We previously demonstrated multimodal ophthalmic imaging using swept-source spectrally encoded SLO and OCT (SS-SESLO-OCT). Here, we present system enhancements and a new optical design that increase our SS-SESLO-OCT data throughput by >7x and field-of-view (FOV) by >4x. A 200 kHz 1060 nm Axsun swept-source was optically buffered to 400 kHz sweep-rate, and SESLO and OCT were simultaneously digitized on dual input channels of a 4 GS/s digitizer at 1.2 GS/s per channel using a custom k-clock. We show in vivo human imaging of the anterior segment out to the limbus and retinal fundus over a >40° FOV. In addition, nine overlapping volumetric SS-SESLO-OCT volumes were acquired under video-rate SESLO preview and guidance. In post-processing, all nine SESLO images and en face projections of the corresponding OCT volumes were mosaicked to show widefield multimodal fundus imaging with a >80° FOV. Concurrent multimodal SS-SESLO-OCT may have applications in clinical diagnostic imaging by enabling aiming, image registration, and multi-field mosaicking and benefit intraoperative imaging by allowing for real-time surgical feedback, instrument tracking, and overlays of computationally extracted image-based surrogate biomarkers of disease.

  11. Optical fundamentals of an adaptive substance-on-surface chemical recognizer

    Science.gov (United States)

    Fauconier, Richard; Ndoye, Mandoye; Montlouis, Webert

    2017-10-01

    The objective is to identify the chemical composition of (isotropic and homogeneous) thin liquid and gel films on various surfaces by their infrared reflectance spectra. A bistatic optical sensing concept is proposed here in which a multi-wavelength laser source and a detector are physically displaced from each other. With the aid of the concept apparatus proposed, key optical variables can be measured in real time. The variables in question (substance thickness, refractive index, etc.) are those whose un-observability causes many types of monostatic sensor (in use today) to give ambiguous identifications. Knowledge of the aforementioned key optical variables would allow an adaptive signal-processing algorithm to make unambiguous identifications of the unknown chemicals by their infrared spectra, despite their variable presentations. The proposed bistatic sensor system consists of an optical transmitter and an optical receiver. The whole system can be mounted on a stable platform. Both the optical transmitter subsystem and the optical receiver subsystem contain auxiliary sensors to determine their relative spatial positions and orientations. For each subsystem, these auxiliary sensors include an orientation sensor, and rotational sensors for absolute angular position. A profilometer-and-machine-vision subsystem is also included. An important aspect of determining the necessary optical variables is an aperture that limits the interrogatory beams to a coherent pair, rejecting those resulting from successive multiple reflections. A set of equations is developed to characterize the propagation of a coherent pair of frequency-modulated thin beams through the system. It is also shown that frequency modulation can produce easily measurable beat frequencies for determination of sample thicknesses on the order of microns to millimeters. Also shown is how the apparatus's polarization features allow it to measure the refractive index of any isotropic, homogeneous dielectric

  12. Testing vision with angular and radial multifocal designs using Adaptive Optics.

    Science.gov (United States)

    Vinas, Maria; Dorronsoro, Carlos; Gonzalez, Veronica; Cortes, Daniel; Radhakrishnan, Aiswaryah; Marcos, Susana

    2017-03-01

    Multifocal vision corrections are increasingly used solutions for presbyopia. In the current study we have evaluated, optically and psychophysically, the quality provided by multizone radial and angular segmented phase designs. Optical and relative visual quality were evaluated using 8 subjects, testing 6 phase designs. Optical quality was evaluated by means of Visual Strehl-based-metrics (VS). The relative visual quality across designs was obtained through a psychophysical paradigm in which images viewed through 210 pairs of phase patterns were perceptually judged. A custom-developed Adaptive Optics (AO) system, including a Hartmann-Shack sensor and an electromagnetic deformable mirror, to measure and correct the eye's aberrations, and a phase-only reflective Spatial Light Modulator, to simulate the phase designs, was developed for this study. The multizone segmented phase designs had 2-4 zones of progressive power (0 to +3D) in either radial or angular distributions. The response of an "ideal observer" purely responding on optical grounds to the same psychophysical test performed on subjects was calculated from the VS curves, and compared with the relative visual quality results. Optical and psychophysical pattern-comparison tests showed that while 2-zone segmented designs (angular & radial) provided better performance for far and near vision, 3- and 4-zone segmented angular designs performed better for intermediate vision. AO-correction of natural aberrations of the subjects modified the response for the different subjects but general trends remained. The differences in perceived quality across the different multifocal patterns are, in a large extent, explained by optical factors. AO is an excellent tool to simulate multifocal refractions before they are manufactured or delivered to the patient, and to assess the effects of the native optics to their performance. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. An adaptive optics aided differential optical positioning for passive orbit determination of the space debris at the geostationary orbit

    Science.gov (United States)

    Piatrou, Piotr; Rigaut, Francois

    2017-10-01

    Proliferation of space debris presents an imminent threat to all space assets. The problem is especially severe for the geostationary band of orbits (GEO) because the GEO objects never leave their orbit and, at the same time, are difficult to observe and operate due to large distance from the Earth. Under the influence of tidal forces, even passive GEO objects achieve high local velocities without vacating GEO positions, which may potentially lead to devastating collisions. Our ability to predict collisions in GEO is limited by the scarcity of the accurate orbital data, especially about the small and passive objects. The efforts to address this omission strongly rely on the ground-based optical sensors and, consequently, on the efficient space object detection and tracking techniques. In this paper we propose a passive differential optical debris tracking technique combining adaptive optics and a high accuracy astrometric references resulting in a significant improvement in the GEO object positioning accuracy. The achievable accuracy is estimated via detailed numerical simulations of two telescopes in different locations.

  14. Ultrahigh-resolution adaptive optics - optical coherence tomography: toward isotropic 3 μm resolution for in vivo retinal imaging

    Science.gov (United States)

    Zawadzki, Robert J.; Zhang, Yan; Jones, Steven M.; Ferguson, R. Daniel; Choi, Stacey S.; Cense, Barry; Evans, Julia W.; Chen, Diana; Miller, Donald T.; Olivier, Scot S.; Werner, John S.

    2007-02-01

    Ultrahigh axial resolution in adaptive optics - optical coherence tomography (AO-OCT) is fundamentally limited by the intrinsic chromatic aberrations of the human eye. Variation in refractive index of the ocular media with wavelength causes the spectral content of broadband light sources to focus at different depths in the retina for light entering the eye and at the imaging detector for light exiting. This effect has not been previously reported for ultrahigh-resolution OCT (without AO) likely because the effect is masked by the relatively long depth of focus dictated by the small pupils used in these systems. With AO, the pupil size is much larger and depth of focus substantially narrower. As such the chromatic aberrations of the eye can counteract the lateral resolution benefit of AO when used with broadband light sources. To more fully tap the potential of AO-OCT, compensation of the eye's chromatic and monochromatic aberrations must occur concurrently. One solution is to insert an achromatizing lens in front of the eye whose chromatic aberrations are equal but opposite in sign to that of the eye. In this paper we evaluate the efficacy of a novel design that uses a custom achromatizing lens placed near the fiber collimating optic. AO-OCT images are acquired on several subjects with and without the achromatizing lens and in combination with two light sources of different spectral width. The combination of the achromatizing lens and broadband light source yielded the sharpest images of the retina and the smallest speckle.

  15. Scanned ion beam therapy for prostate carcinoma. Comparison of single plan treatment and daily plan-adapted treatment

    Energy Technology Data Exchange (ETDEWEB)

    Hild, Sebastian [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, Department of Biophysics, Darmstadt (Germany); University Clinic Erlangen and Friedrich- Alexander-University Erlangen-Nuernberg (FAU), Department of Radiation Oncology, Erlangen (Germany); Graeff, Christian [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, Department of Biophysics, Darmstadt (Germany); Rucinski, Antoni [University Clinic Heidelberg, Heidelberg Ion-Beam Therapy Center (HIT) and Department of Radiation Oncology, Heidelberg (Germany); Sapienza Universit' a di Roma, Dipartimento di Scienze di Base e Applicate per Ingegneria, Roma (Italy); INFN, Roma (Italy); Zink, Klemens [University of Applied Sciences, Institute for Medical Physics and Radiation Protection, Giessen (Germany); University Medical Center Giessen-Marburg, Department of Radiotherapy and Radiooncology, Marburg (Germany); Habl, Gregor [University Clinic Heidelberg, Heidelberg Ion-Beam Therapy Center (HIT) and Department of Radiation Oncology, Heidelberg (Germany); Klinikum rechts der Isar, Technische Universitaet Muenchen (TUM), Department of Radiation Oncology, Munich (Germany); Durante, Marco [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, Department of Biophysics, Darmstadt (Germany); Technische Universitaet Darmstadt, Faculty of Physics, Darmstadt (Germany); Herfarth, Klaus [University Clinic Heidelberg, Heidelberg Ion-Beam Therapy Center (HIT) and Department of Radiation Oncology, Heidelberg (Germany); Bert, Christoph [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, Department of Biophysics, Darmstadt (Germany); University Clinic Erlangen and Friedrich- Alexander-University Erlangen-Nuernberg (FAU), Department of Radiation Oncology, Erlangen (Germany); University Hospital Erlangen, Radiation Oncology, Erlangen (Germany)

    2016-02-15

    Intensity-modulated particle therapy (IMPT) for tumors showing interfraction motion is a topic of current research. The purpose of this work is to compare three treatment strategies for IMPT to determine potential advantages and disadvantages of ion prostate cancer therapy. Simulations for three treatment strategies, conventional one-plan radiotherapy (ConvRT), image-guided radiotherapy (IGRT), and online adaptive radiotherapy (ART) were performed employing a dataset of 10 prostate cancer patients with six CT scans taken at one week intervals. The simulation results, using a geometric margin concept (7-2 mm) as well as patient-specific internal target volume definitions for IMPT were analyzed by target coverage and exposure of critical structures on single fraction dose distributions. All strategies led to clinically acceptable target coverage in patients exhibiting small prostate motion (mean displacement < 4 mm), but IGRT and especially ART led to significant sparing of the rectum. In 20 % of the patients, prostate motion exceeded 4 mm causing insufficient target coverage for ConvRT (V95{sub mean} = 0.86, range 0.63-0.99) and IGRT (V95{sub mean} = 0.91, range 0.68-1.00), while ART maintained acceptable target coverage. IMPT of prostate cancer demands consideration of rectal sparing and adaptive treatment replanning for patients exhibiting large prostate motion. (orig.) [German] Adaptive Therapieansaetze fuer sich interfraktionell bewegende Zielvolumina in der intensitaetsmodulierten Partikeltherapie (IMPT) befinden sich zurzeit in der Entwicklung. In dieser Arbeit werden drei Behandlungsstrategien auf moegliche Vor- und Nachteile in der IMPT des Prostatakarzinoms hin untersucht. Auf Basis eines anonymisierten Datensatzes aus 10 Patienten mit Prostatakarzinom wurden die drei Bestrahlungsstrategien, konventionelle Ein-Plan-Strahlentherapie (ConvRT), bildunterstuetzte Strahlentherapie (IGRT) und tagesaktuelle Strahlentherapie (adaptive radiotherapy,ART), simuliert

  16. Optical surface scanning for respiratory motion monitoring in radiotherapy: a feasibility study

    DEFF Research Database (Denmark)

    Bekke, Susanne Lise; Mahmood, Faisal; Helt-Hansen, Jakob

    2014-01-01

    Purpose. We evaluated the feasibility of a surface scanning system (Catalyst) for respiratory motion monitoring of breast cancer patients treated with radiotherapy in deep inspiration breath-hold (DIBH). DIBH is used to reduce the radiation dose to the heart and lung. In contrast to RPM......, a competing marker-based system, Catalyst does not require an objectmarker on the patient's skin. Materials and Methods. Experiment 1: a manikin was used to simulate sinusoidal breathing. The amplitude, period and baseline (signal value at end-expiration) were estimated with RPM and Catalyst. Experiment 2...... and 3: the Quasar phantom was used to study if the angle of the monitored surface affects the amplitude of the recorded signal. Results. Experiment 1: we observed comparable period estimates for both systems. The amplitudes were 8 ± 0.1 mm (Catalyst) and 4.9 ± 0.1 mm (RPM). Independent check with in...

  17. Ultra-High Vacuum Compatible Optical Chopper System for Synchrotron X-ray Scanning Tunneling Microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Hao; Cummings, Marvin L.; Shirato, Nozomi; Stripe, Benjamin D.; Rosenmann, Daniel; Preissner, Curt A.; Freeland, John W.; Kersell, Heath R.; Hla, Saw Wai; Rose, Volker

    2015-01-01

    High-speed beam choppers are a crucial part of time-resolved x-ray studies as well as a necessary component to enable elemental contrast in synchrotron x-ray scanning tunneling microscopy (SX-STM). However, many chopper systems are not capable of operation in vacuum, which restricts their application to x-ray studies with high photon energies, where air absorption does not present a significant problem. To overcome this limitation, we present a fully ultra-high vacuum (UHV) compatible chopper system capable of operating at variable chopping frequencies up to 4 kHz. The lightweight aluminum chopper disk is coated with Ti and Au films to provide the required beam attenuation for soft and hard x-rays with photon energies up to about 12 keV. The chopper is used for lock-in detection of x-ray enhanced signals in SX-STM.

  18. Ultra-high vacuum compatible optical chopper system for synchrotron x-ray scanning tunneling microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Hao, E-mail: hc000211@ohio.edu [Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439 (United States); Nanoscale and Quantum Phenomena Institute, Physics & Astronomy Department, Ohio University, Athens, Ohio 45701 (United States); Cummings, Marvin; Shirato, Nozomi; Stripe, Benjamin; Preissner, Curt; Freeland, John W. [Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439 (United States); Rosenmann, Daniel [Center for Nanoscale Materials, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439 (United States); Kersell, Heath; Hla, Saw-Wai [Nanoscale and Quantum Phenomena Institute, Physics & Astronomy Department, Ohio University, Athens, Ohio 45701 (United States); Center for Nanoscale Materials, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439 (United States); Rose, Volker, E-mail: vrose@anl.gov [Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439 (United States); Center for Nanoscale Materials, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439 (United States)

    2016-01-28

    High-speed beam choppers are a crucial part of time-resolved x-ray studies as well as a necessary component to enable elemental contrast in synchrotron x-ray scanning tunneling microscopy (SX-STM). However, many chopper systems are not capable of operation in vacuum, which restricts their application to x-ray studies with high photon energies, where air absorption does not present a significant problem. To overcome this limitation, we present a fully ultra-high vacuum (UHV) compatible chopper system capable of operating at variable chopping frequencies up to 4 kHz. The lightweight aluminum chopper disk is coated with Ti and Au films to provide the required beam attenuation for soft and hard x-rays with photon energies up to about 12 keV. The chopper is used for lock-in detection of x-ray enhanced signals in SX-STM.

  19. High-Contrast Imaging using Adaptive Optics for Extrasolar Planet Detection

    Energy Technology Data Exchange (ETDEWEB)

    Evans, Julia Wilhelmsen [Univ. of California, Davis, CA (United States)

    2006-01-01

    Direct imaging of extrasolar planets is an important, but challenging, next step in planetary science. Most planets identified to date have been detected indirectly--not by emitted or reflected light but through the effect of the planet on the parent star. For example, radial velocity techniques measure the doppler shift in the spectrum of the star produced by the presence of a planet. Indirect techniques only probe about 15% of the orbital parameter space of our solar system. Direct methods would probe new parameter space, and the detected light can be analyzed spectroscopically, providing new information about detected planets. High contrast adaptive optics systems, also known as Extreme Adaptive Optics (ExAO), will require contrasts of between 10-6 and 10-7 at angles of 4-24 λ/D on an 8-m class telescope to image young Jupiter-like planets still warm with the heat of formation. Contrast is defined as the intensity ratio of the dark wings of the image, where a planet might be, to the bright core of the star. Such instruments will be technically challenging, requiring high order adaptive optics with > 2000 actuators and improved diffraction suppression. Contrast is ultimately limited by residual static wavefront errors, so an extrasolar planet imager will require wavefront control with an accuracy of better than 1 nm rms within the low- to mid-spatial frequency range. Laboratory demonstrations are critical to instrument development. The ExAO testbed at the Laboratory for Adaptive Optics was designed with low wavefront error and precision optical metrology, which is used to explore contrast limits and develop the technology needed for an extrasolar planet imager. A state-of-the-art, 1024-actuator micro-electrical-mechanical-systems (MEMS) deformable mirror was installed and characterized to provide active wavefront control and test this novel technology. I present 6.5 x 10-8 contrast measurements with a prolate shaped pupil and

  20. An analysis of the optics of a field ionization ion source for application with a scanning proton microprobe

    Science.gov (United States)

    Colman, R. A.; Allan, G. L.; Legge, G. J. F.

    1992-12-01

    This article analyzes a field ionization source for use within a pelletron accelerator which provides the primary beam for a scanning proton microprobe. The charge simulation method is used to calculate the electrostatic field, and ray tracing is used to determine optical properties. Current characteristics are taken from experimental results. Gaussian properties indicate an effective source radius of below 10-3 μm at low angles. Chromatic aberration is calculated by perturbing initial particle energies, then tracing back from field-free trajectories. Calculations indicate that at typical source voltages, the beam is never chromatically limited. Spherical aberration is also calculated and the source is found to be spherically limited above a divergence of approximately 0.1 rad. Finally, calculations indicate that a brightness of 106 A m-2 rad-2 V-1 is achieved by the source producing 150 pA of current at a tip electric field of 25 V/nm.

  1. Innovative measurement of parallelism for parallel transparent plate based on optical scanning holography by using a random-phase pupil.

    Science.gov (United States)

    Luo-Zhi, Zhang; Jian-Ping, Hu; Dao-Ming, Wan; Xing, Zeng; Chun-Miao, Li; Xin, Zhou

    2015-03-20

    A potential method is proposed to measure the parallelism of parallel transparent plate with an advanced lower limit and a convenient process by optical scanning holography (OSH) using a random-phase pupil, which is largely distinct from traditional methods. As a new possible application of OSH, this promising method is demonstrated theoretically and numerical simulations are carried out on a 2  cm×2  cm parallel plate. Discussions are also made on the quality of reconstructed image as well as local mean square error (MSE), which are closely related with the parallelism of sample. These amounts may become the judgments of parallelism, while in most interference methods judgments are paces between two interference fringes. In addition, randomness of random-phase pupil also affects the quality of reconstructed image and local MSE. According to the simulation results, high parallelism usually brings about distinguishable reconstructed information and suppressed local MSE.

  2. A study of internal oxidation in carburized steels by glow discharge optical emission spectroscopy and scanning electron microscopy

    CERN Document Server

    An, X; Rainforth, W M; Chen, L

    2003-01-01

    The internal oxidation of Cr-Mn carburizing steel was studied. Internal oxidation was induced using a commercial carburizing process. Sputter erosion coupled with glow discharge optical emission spectroscopy (GDOES) was used to determine the depth profile elemental distribution within the internal oxidation layer (<10 mu m). In addition, scanning electron microscopy (SEM) equipped with energy dispersive spectrometer (EDS) studies were carried out on selected sputter eroded surfaces. Oxide type was identified primarily by transmission electron microscopy (TEM). The carburized surface was found to consist of a continuous oxide layer, followed by a complex internal oxidation layer, where Cr and Mn oxides were found to populate grain boundaries in a globular form in the near surface region. At greater depths (5-10 mu m), Si oxides formed as a grain boundary network. The internal oxides (mainly complex oxides) grew quickly during the initial stages of the carburizing process (2 h, 800 deg. C+3 h, 930 deg. C). G...

  3. Complex nano-patterning of structural, optical, electrical and electron emission properties of amorphous silicon thin films by scanning probe

    Science.gov (United States)

    Fait, Jan; Čermák, Jan; Stuchlík, Jiří; Rezek, Bohuslav

    2018-01-01

    Preparation of nanoscale templates represents an important step for synthesis and assembly of diverse nanostructures and nanoscale devices. We show that complex nano-structural templates in a thin (40 nm) layer of hydrogenated amorphous silicon (a-Si:H) can be prepared by using locally applied electric field in an atomic force microscope (AFM). Depth of the resulting structures (1-40 nm) can be controlled by the process parameters (magnitude of electric field, exposure time, or nano-sweeping of the tip). We demonstrate that complex patterns can be scribed into the a-Si:H layer in that way. The prepared patterns exhibit different structural, optical, electrical, and electron emission properties, compared to the surroundings as detected by Raman micro-spectroscopy, scanning electron microscopy (SEM), and conductive AFM. The silicon thin films with locally modified properties can be useful in themselves or can serve as templates for further nanoscale growth or assembly.

  4. Miniature endoscopic optical coherence tomography probe employing a two-axis microelectromechanical scanning mirror with through-silicon vias.

    Science.gov (United States)

    Liu, Lin; Wu, Lei; Sun, Jingjing; Lin, Elaine; Xie, Huikai

    2011-02-01

    We present the design and experimental results of a new MEMS-based endoscopic optical coherence tomography (OCT) probe. The uniqueness of this miniature OCT imaging probe is a two-axis MEMS mirror with through-silicon vias (TSVs) for interconnecting. The TSV interconnection enables ultracompact probe design, successfully reducing the probe size to only 2.6 mm in diameter. The MEMS mirror is actuated by an electrothermal actuator that is capable of scanning ± 16° at only 3.6 V DC. Two-dimensional and three-dimensional OCT images of microspheres embedded in PDMS and acute rat brain tissue have been obtained with this miniature probe in a time-domain OCT system.

  5. Volumetric rendering and metrology of spherical gradient refractive index lens imaged by angular scan optical coherence tomography system.

    Science.gov (United States)

    Yao, Jianing; Thompson, Kevin P; Ma, Bin; Ponting, Michael; Rolland, Jannick P

    2016-08-22

    In this paper, we develop the methodology, including the refraction correction, geometrical thickness correction, coordinate transformation, and layer segmentation algorithms, for 3D rendering and metrology of a layered spherical gradient refractive index (S-GRIN) lens based on the imaging data collected by an angular scan optical coherence tomography (OCT) system. The 3D mapping and rendering enables direct 3D visualization and internal defect inspection of the lens. The metrology provides assessment of the surface geometry, the lens thickness, the radii of curvature of the internal layer interfaces, and the misalignment of the internal S-GRIN distribution with respect to the lens surface. The OCT metrology results identify the manufacturing defects, and enable targeted process development for optimizing the manufacturing parameters. The newly fabricated S-GRIN lenses show up to a 7x spherical aberration reduction that allows a significantly increased utilizable effective aperture.

  6. High-resolution in-depth imaging of optically cleared thick samples using an adaptive SPIM

    Science.gov (United States)

    Masson, Aurore; Escande, Paul; Frongia, Céline; Clouvel, Grégory; Ducommun, Bernard; Lorenzo, Corinne

    2015-11-01

    Today, Light Sheet Fluorescence Microscopy (LSFM) makes it possible to image fluorescent samples through depths of several hundreds of microns. However, LSFM also suffers from scattering, absorption and optical aberrations. Spatial variations in the refractive index inside the samples cause major changes to the light path resulting in loss of signal and contrast in the deepest regions, thus impairing in-depth imaging capability. These effects are particularly marked when inhomogeneous, complex biological samples are under study. Recently, chemical treatments have been developed to render a sample transparent by homogenizing its refractive index (RI), consequently enabling a reduction of scattering phenomena and a simplification of optical aberration patterns. One drawback of these methods is that the resulting RI of cleared samples does not match the working RI medium generally used for LSFM lenses. This RI mismatch leads to the presence of low-order aberrations and therefore to a significant degradation of image quality. In this paper, we introduce an original optical-chemical combined method based on an adaptive SPIM and a water-based clearing protocol enabling compensation for aberrations arising from RI mismatches induced by optical clearing methods and acquisition of high-resolution in-depth images of optically cleared complex thick samples such as Multi-Cellular Tumour Spheroids.

  7. Multi-volumetric registration and mosaicking using swept-source spectrally encoded scanning laser ophthalmoscopy and optical coherence tomography

    Science.gov (United States)

    Bozic, Ivan; El-Haddad, Mohamed T.; Malone, Joseph D.; Joos, Karen M.; Patel, Shriji N.; Tao, Yuankai K.

    2017-02-01

    Ophthalmic diagnostic imaging using optical coherence tomography (OCT) is limited by bulk eye motions and a fundamental trade-off between field-of-view (FOV) and sampling density. Here, we introduced a novel multi-volumetric registration and mosaicking method using our previously described multimodal swept-source spectrally encoded scanning laser ophthalmoscopy and OCT (SS-SESLO-OCT) system. Our SS-SESLO-OCT acquires an entire en face fundus SESLO image simultaneously with every OCT cross-section at 200 frames-per-second. In vivo human retinal imaging was performed in a healthy volunteer, and three volumetric datasets were acquired with the volunteer moving freely and refixating between each acquisition. In post-processing, SESLO frames were used to estimate en face rotational and translational motions by registering every frame in all three volumetric datasets to the first frame in the first volume. OCT cross-sections were contrast-normalized and registered axially and rotationally across all volumes. Rotational and translational motions calculated from SESLO frames were applied to corresponding OCT B-scans to compensate for interand intra-B-scan bulk motions, and the three registered volumes were combined into a single interpolated multi-volumetric mosaic. Using complementary information from SESLO and OCT over serially acquired volumes, we demonstrated multivolumetric registration and mosaicking to recover regions of missing data resulting from blinks, saccades, and ocular drifts. We believe our registration method can be directly applied for multi-volumetric motion compensation, averaging, widefield mosaicking, and vascular mapping with potential applications in ophthalmic clinical diagnostics, handheld imaging, and intraoperative guidance.

  8. Novel applications of an adaptive optics visual simulator in the clinical setting

    Science.gov (United States)

    Krueger, Ronald R.; Abdala, Alexandra; Rocha, Karolinne; Chateau, Nicolas; Vabre, Laurent

    2010-02-01

    Purpose: To evaluate the clinical benefit of using an adaptive optics visual simulator (AOVS) and its impact in different clinical settings. Methods: An adaptive optics visual simulator performed the experimental procedure and was used to optically introduce aberrations in 9 normal eyes for visual acuity (VA) change, and in 10 cyclopleged eyes for enhancing depth of focus (DoF). AOVS was also used to correct 20 highly aberrated eyes. Results: The correction/induction of high order aberrations (HOA) alters the best-corrected visual acuity (BCVA) by a mean of ~1 to 1.5 lines compared to the best spectacle correction. The depth of focus (DoF) was most enhanced (~2.0 D) with the introduction of negative and positive spherical aberration of 0.6 μm magnitude. The correction of HOAs in highly aberrated eyes improved BCVA by a mean of ~1.5 to 2 lines in two groups of pathological eyes. Conclusions: Aberrations have differing effects according to their clinical use. The AOVS defines the clinical response of HOAs on VA, visual perceptions and DoF.

  9. Automated Fovea Detection in Spectral Domain Optical Coherence Tomography Scans of Exudative Macular Disease

    Directory of Open Access Journals (Sweden)

    Jing Wu

    2016-01-01

    Full Text Available In macular spectral domain optical coherence tomography (SD-OCT volumes, detection of the foveal center is required for accurate and reproducible follow-up studies, structure function correlation, and measurement grid positioning. However, disease can cause severe obscuring or deformation of the fovea, thus presenting a major challenge in automated detection. We propose a fully automated fovea detection algorithm to extract the fovea position in SD-OCT volumes of eyes with exudative maculopathy. The fovea is classified into 3 main appearances to both specify the detection algorithm used and reduce computational complexity. Based on foveal type classification, the fovea position is computed based on retinal nerve fiber layer thickness. Mean absolute distance between system and clinical expert annotated fovea positions from a dataset comprised of 240 SD-OCT volumes was 162.3 µm in cystoid macular edema and 262 µm in nAMD. The presented method has cross-vendor functionality, while demonstrating accurate and reliable performance close to typical expert interobserver agreement. The automatically detected fovea positions may be used as landmarks for intra- and cross-patient registration and to create a joint reference frame for extraction of spatiotemporal features in “big data.” Furthermore, reliable analyses of retinal thickness, as well as retinal structure function correlation, may be facilitated.

  10. Scanning Optical Head with Nontilted Reference Beam: Assuring Nanoradian Accuracy for a New Generation Surface Profiler in the Large-Slope Testing Range

    Directory of Open Access Journals (Sweden)

    Shinan Qian

    2011-01-01

    Full Text Available Nanoradian Surface Profilers (NSPs are required for state-of-the-art synchrotron radiation optics and high-precision optical measurements. Nano-radian accuracy must be maintained in the large-angle test range. However, the beams' notable lateral motions during tests of most operating profilers, combined with the insufficiencies of their optical components, generate significant errors of ∼1 μrad rms in the measurements. The solution to nano-radian accuracy for the new generation of surface profilers in this range is to apply a scanning optical head, combined with nontilted reference beam. I describe here my comparison of different scan modes and discuss some test results.

  11. An adaptive scan of high frequency subbands for dyadic intra frame in MPEG4-AVC/H.264 scalable video coding

    Science.gov (United States)

    Shahid, Z.; Chaumont, M.; Puech, W.

    2009-01-01

    This paper develops a new adaptive scanning methodology for intra frame scalable coding framework based on a subband/wavelet(DWTSB) coding approach for MPEG-4 AVC/H.264 scalable video coding (SVC). It attempts to take advantage of the prior knowledge of the frequencies which are present in different higher frequency subbands. We propose dyadic intra frame coding method with adaptive scan (DWTSB-AS) for each subband as traditional zigzag scan is not suitable for high frequency subbands. Thus, by just modification of the scan order of the intra frame scalable coding framework of H.264, we can get better compression. The proposed algorithm has been theoretically justified and is thoroughly evaluated against the current SVC test model JSVM and DWTSB through extensive coding experiments for scalable coding of intra frame. The simulation results show the proposed scanning algorithm consistently outperforms JSVM and DWTSB in PSNR performance. This results in extra compression for intra frames, along with spatial scalability. Thus Image and video coding applications, traditionally serviced by separate coders, can be efficiently provided by an integrated coding system.

  12. Modified artificial fish school algorithm for free space optical communication with sensor-less adaptive optics system

    Science.gov (United States)

    Cao, Jingtai; Zhao, Xiaohui; Li, Zhaokun; Liu, Wei; Gu, Haijun

    2017-11-01

    The performance of free space optical (FSO) communication system is limited by atmospheric turbulent extremely. Adaptive optics (AO) is the significant method to overcome the atmosphere disturbance. Especially, for the strong scintillation effect, the sensor-less AO system plays a major role for compensation. In this paper, a modified artificial fish school (MAFS) algorithm is proposed to compensate the aberrations in the sensor-less AO system. Both the static and dynamic aberrations compensations are analyzed and the performance of FSO communication before and after aberrations compensations is compared. In addition, MAFS algorithm is compared with artificial fish school (AFS) algorithm, stochastic parallel gradient descent (SPGD) algorithm and simulated annealing (SA) algorithm. It is shown that the MAFS algorithm has a higher convergence speed than SPGD algorithm and SA algorithm, and reaches the better convergence value than AFS algorithm, SPGD algorithm and SA algorithm. The sensor-less AO system with MAFS algorithm effectively increases the coupling efficiency at the receiving terminal with fewer numbers of iterations. In conclusion, the MAFS algorithm has great significance for sensor-less AO system to compensate atmospheric turbulence in FSO communication system.

  13. Repeatability of a Commercially Available Adaptive Optics Visual Simulator and Aberrometer in Normal and Keratoconic Eyes.

    Science.gov (United States)

    Shetty, Rohit; Kochar, Shruti; Grover, Tushar; Khamar, Pooja; Kusumgar, Pallak; Sainani, Kanchan; Sinha Roy, Abhijit

    2017-11-01

    To evaluate the repeatability of aberration measurement obtained by a Hartmann-Shack aberrometer combined with a visual adaptive optics simulator in normal and keratoconic eyes. One hundred fifteen normal eyes and 92 eyes with grade I and II keratoconus, as per the Amsler-Krumeich classification, were included in the study. To evaluate the repeatability, three consecutive measurements of ocular aberrations were obtained by a single operator. Zernike analyses up to the 5th order for a pupil size of 4.5 mm were performed. Statistical analyses included the intraclass correlation coefficient (ICC) and within-subject standard deviation (SD). For intrasession repeatability, the ICC value for sphere and cylinder was 0.94 and 0.93 in normal eyes and 0.98 and 0.97 in keratoconic eyes, respectively. The ICC for root mean square of higher order aberrations (HOARMS) was 0.82 in normal and 0.98 in keratoconic eyes. For 3rd order aberrations (trefoil and coma), the ICC values were greater than 0.87 for normal eyes and greater than 0.92 for keratoconic eyes. The ICC for spherical aberration was 0.92 and 0.90 in normal and keratoconic eyes, respectively. Visual adaptive optics provided repeatable aberrometry data in both normal and keratoconic eyes. For most of the parameters, the repeatability in eyes with early keratoconus was somewhat better than that for normal eyes. The repeatability of the Zernike terms was acceptable for 3rd order (trefoil and coma) and spherical aberrations. Therefore, visual adaptive optics was a suitable tool to perform repeatable aberrometric measurements. [J Refract Surg. 2017;33(11):769-772.]. Copyright 2017, SLACK Incorporated.

  14. Immature visual neural system in children reflected by contrast sensitivity with adaptive optics correction

    Science.gov (United States)

    Liu, Rong; Zhou, Jiawei; Zhao, Haoxin; Dai, Yun; Zhang, Yudong; Tang, Yong; Zhou, Yifeng

    2014-01-01

    This study aimed to explore the neural development status of the visual system of children (around 8 years old) using contrast sensitivity. We achieved this by eliminating the influence of higher order aberrations (HOAs) with adaptive optics correction. We measured HOAs, modulation transfer functions (MTFs) and contrast sensitivity functions (CSFs) of six children and five adults with both corrected and uncorrected HOAs. We found that when HOAs were corrected, children and adults both showed improvements in MTF and CSF. However, the CSF of children was still lower than the adult level, indicating the difference in contrast sensitivity between groups cannot be explained by differences in optical factors. Further study showed that the difference between the groups also could not be explained by differences in non-visual factors. With these results we concluded that the neural systems underlying vision in children of around 8 years old are still immature in contrast sensitivity. PMID:24732728

  15. Concept for SAMOS: SOAR Adaptive-optics Multi-object Spectrograph

    Science.gov (United States)

    Robberto, Massimo; Donahue, M.; Tokovinin, A.; Smee, S.; Barkhauser, R.; Deustua, S.; Gennaro, M.; Kalirai, J.; MacKenty, J.; Pontoppidan, K.

    2014-01-01

    We present the concept design of SAMOS, a Multi-Object Spectrograph for the laser-guided adaptive-optics module SAM of the 4.2m telescope SOAR at Cerro Pachon (Chile). SAMOS exploits the improved optical quality at visible wavelength over a 3'x3' field of view delivered by SAM to simultaneously acquire hundreds of spectra of rich, crowded fields. Slits are generated "on-the-fly" using a MEMS device, namely a last-generation Digital Micro-Mirror Device (DMD) produced by Texas Instrument. The use of a DMD allows optimization of the slit width to the local seeing conditions and to the variable, field-dependent image sharpness. We illustrate a sample of the science investigations enabled by SAMOS and a baseline scheme of the system at the SAM station.

  16. Data-aided adaptive weighted channel equalizer for coherent optical OFDM.

    Science.gov (United States)

    Mousa-Pasandi, Mohammad E; Plant, David V

    2010-02-15

    We report an adaptive weighted channel equalizer (AWCE) for orthogonal frequency division multiplexing (OFDM) and study its performance for long-haul coherent optical OFDM (CO-OFDM) transmission systems. This equalizer updates the equalization parameters on a symbol-by-symbol basis thus can track slight drifts of the optical channel. This is suitable to combat polarization mode dispersion (PMD) degradation while increasing the periodicity of pilot symbols which can be translated into a significant overhead reduction. Furthermore, AWCE can increase the precision of RF-pilot enabled phase noise estimation in the presence of noise, using data-aided phase noise estimation. Simulation results corroborate the capability of AWCE in both overhead reduction and improving the quality of the phase noise compensation (PNC).

  17. A 3.4-mm beam diameter system for retinal imaging with OCT and adaptive optics

    Science.gov (United States)

    Reddikumar, Maddipatla; Cense, Barry

    2017-04-01

    We present an adaptive-optics optical coherence tomography (AO-OCT) system with 3.4-mm beam diameter. A deformable mirror is used for the correction of two radial Zernike orders (defocus, vertical and oblique astigmatism). The aberrations are corrected sequentially with a Shack-Hartmann wave-front sensor and the deformable mirror. This system fills a gap between a standard clinical 1.2-mm beam diameter OCT system and a 6-mm beam diameter AO-OCT system. We also present 8° by 8° en face OCT images from a patient with macular degeneration. This system has a 25 cm by 50 cm footprint, which makes it considerably smaller to conventional 6-mm beam diameter AO-OCT system. Because of its larger field of view and smaller size, it is likely to be useful in the ophthalmic clinics for high-resolution imaging of the human eye retina.

  18. Delay-aware adaptive sleep mechanism for green wireless-optical broadband access networks

    Science.gov (United States)

    Wang, Ruyan; Liang, Alei; Wu, Dapeng; Wu, Dalei

    2017-07-01

    Wireless-Optical Broadband Access Network (WOBAN) is capacity-high, reliable, flexible, and ubiquitous, as it takes full advantage of the merits from both optical communication and wireless communication technologies. Similar to other access networks, the high energy consumption poses a great challenge for building up WOBANs. To shot this problem, we can make some load-light Optical Network Units (ONUs) sleep to reduce the energy consumption. Such operation, however, causes the increased packet delay. Jointly considering the energy consumption and transmission delay, we propose a delay-aware adaptive sleep mechanism. Specifically, we develop a new analytical method to evaluate the transmission delay and queuing delay over the optical part, instead of adopting M/M/1 queuing model. Meanwhile, we also analyze the access delay and queuing delay of the wireless part. Based on such developed delay models, we mathematically derive ONU's optimal sleep time. In addition, we provide numerous simulation results to show the effectiveness of the proposed mechanism.

  19. Weekly Glacier Flow Estimation from Dense Satellite Time Series Using Adapted Optical Flow Technology

    Directory of Open Access Journals (Sweden)

    Bas Altena

    2017-06-01

    Full Text Available Contemporary optical remote sensing satellites or constellations of satellites can acquire imagery at sub-weekly or even daily timescales. These systems have the potential to facilitate intra-seasonal, short-term surface velocity variations across a range of ice masses. Current techniques for displacement estimation are based on matching image pairs with sufficient displacement and/or preservation of the surface over time and consequently, do not benefit from an increase in satellite revisit times. Here, we explore an approach that is fundamentally different from image correlation or similar approaches and engages the concept of optical flow. Our goal is to assess whether this technique could overcome the limitations of image matching and yield new insights in glacier flow dynamics. We implement two different methods of optical flow, and test these implementations utilizing the SPOT5 Take5 dataset at two glaciers: Kronebreen, Svalbard and Kaskawulsh Glacier, Yukon. At Kaskawulsh Glacier, we extract intra-seasonal velocity variations that are synchronous with episodes of increased air temperature. Moreover, even for the cloudy dataset of Kronebreen, we can extract spatio-temporal trajectories that correlate well with measured GPS flow paths. Since the underlying concept is simple and computationally efficient due to data-reduction, our optical flow methodology can be rapidly adapted for a range of studies from the investigation of large scale ice sheet dynamics down to the estimation of displacements over small and slow flowing glaciers.

  20. Adaptive Electronic Dispersion Compensator for Chromatic and Polarization-Mode Dispersions in Optical Communication Systems

    Directory of Open Access Journals (Sweden)

    Ut-Va Koc

    2005-06-01

    Full Text Available The widely-used LMS algorithm for coefficient updates in adaptive (feedforward/decision-feedback equalizers is found to be suboptimal for ASE-dominant systems but various coefficient-dithering approaches suffer from slow adaptation rate without guarantee of convergence. In view of the non-Gaussian nature of optical noise after the square-law optoelectronic conversion, we propose to apply the higher-order least-mean 2Nth-order (LMN algorithms resulting in OSNR penalty which is 1.5–2 dB less than that of LMS. Furthermore, combined with adjustable slicer threshold control, the proposed equalizer structures are demonstrated through extensive Monte Carlo simulations to achieve better performance.