WorldWideScience

Sample records for activity programme kiss

  1. Design, synthesis, and biological evaluation of novel investigational nonapeptide KISS1R agonists with testosterone-suppressive activity.

    Science.gov (United States)

    Asami, Taiji; Nishizawa, Naoki; Matsui, Hisanori; Nishibori, Kimiko; Ishibashi, Yoshihiro; Horikoshi, Yasuko; Nakayama, Masaharu; Matsumoto, Shin-ichi; Tarui, Naoki; Yamaguchi, Masashi; Matsumoto, Hirokazu; Ohtaki, Tetsuya; Kitada, Chieko

    2013-11-14

    Metastin/kisspeptin is a 54 amino acid peptide ligand of the KISS1R receptor and is a critical regulator of GnRH secretion. The N-terminally truncated peptide, metastin(45-54), possesses a 10-fold higher receptor-binding affinity than full-length metastin and agonistic KISS1R activity but is rapidly inactivated in rodent plasma. We have developed a decapeptide analog [D-Tyr(45),D-Trp(47),azaGly(51),Arg(Me)(53)]metastin(45-54) with improved serum stability compared with metastin(45-54) but with decreased KISS1R agonistic activity. Amino acid replacements at positions 45-47 led to an enhancement of KISS1R agonistic activity and metabolic stability. N-terminal truncation resulted in a stable nonapeptide, [D-Tyr(46),D-Pya(4)(47),azaGly(51),Arg(Me)(53)]metastin(46-54), compound 26, which displayed KISS1R binding affinities comparable to metastin(45-54) and had improved serum stability. Compound 26 reduced plasma testosterone in male rats and is the first short-length metastin analog to possess testosterone suppressive activities. Compound 26 has led to the elucidation of investigational analogs TAK-683 and TAK-448, both of which have undergone clinical evaluation for hormone-dependent diseases such as prostate cancer.

  2. In Vitro Activities of Kissorphin, a Novel Hexapeptide KiSS-1 Derivative, in Neuronal Cells

    Directory of Open Access Journals (Sweden)

    Nathaniel G. N. Milton

    2012-01-01

    Full Text Available The primary products of the metastasis-suppressor KiSS-1 gene are the kisspeptin (KP peptides that stimulate gonadotrophin-releasing-hormone (GnRH release via GPR-54 receptor activation. Recent studies have suggested that the KP-10 peptide also activates neuropeptide FF (NPFF receptors. The aim of the current study was to determine the activities of the KiSS-1 derivative kissorphin (KSO, which contains the first six amino acids of the KP-10 peptide, is C-terminally amidated, and shares amino acid similarities with the biologically active NPFF 3–8 sequence. The KSO peptide inhibited forskolin-stimulated cyclic adenosine monophosphate (cAMP production in ND7/23 neuroblastoma cells via an action that could be inhibited by the NPFF receptor antagonist RF9. Release of GnRH by LA-N-1 neuroblastoma cells was not altered by the KSO peptide. In ND7/23 neuroblastoma cells, the KSO peptide was able to reduce forskolin neuroprotection against H2O2 toxicity. The KSO peptide was also able to prevent prostaglandin E2-induced apoptosis in rat cortical neurons. The NPFF receptor antagonist RF9 could inhibit these actions of the KSO peptide in oxidative stress and apoptosis models. In conclusion, the kissorphin peptide, comprising the amino acid sequence Tyr-Asn-Trp-Asn-Ser-Phe-NH2, has NPFF-like biological activity without showing any GnRH releasing activity and inhibits forskolin-activated cAMP release.

  3. Microarray of programmable electrochemically active elements

    DEFF Research Database (Denmark)

    McCaskill, John; Maeke, Thomas; Straczek, Lukas

    Possible applications of the MICREAgents Dock, a two dimensional array of programmable electrochemically active elements, to Alife.......Possible applications of the MICREAgents Dock, a two dimensional array of programmable electrochemically active elements, to Alife....

  4. Microarray of programmable electrochemically active elements

    DEFF Research Database (Denmark)

    S. McCaskill, John; Maeke, Thomas; Straczek, Lukas;

    2016-01-01

    This paper describes possible applications of a two dimensional array of programmable electrochemically active elements to Alife. The array has been developed as part of the MICREA-gents project, and after several design phases, is now a mature enough device for general use beyond the project. Here...

  5. Microarray of programmable electrochemically active elements

    DEFF Research Database (Denmark)

    S. McCaskill, John; Maeke, Thomas; Straczek, Lukas

    2016-01-01

    This paper describes possible applications of a two dimensional array of programmable electrochemically active elements to Alife. The array has been developed as part of the MICREA-gents project, and after several design phases, is now a mature enough device for general use beyond the project. Here...

  6. Prevalence of KISS1 Receptor mutations in a series of 603 patients with normosmic congenital hypogonadotrophic hypogonadism and characterization of novel mutations: a single-centre study.

    Science.gov (United States)

    Francou, Bruno; Paul, Charlotte; Amazit, Larbi; Cartes, Alejandra; Bouvattier, Claire; Albarel, Frédérique; Maiter, Dominique; Chanson, Philippe; Trabado, Séverine; Brailly-Tabard, Sylvie; Brue, Thierry; Guiochon-Mantel, Anne; Young, Jacques; Bouligand, Jérôme

    2016-06-01

    What is the exact prevalence of Kisspeptin Receptor (KISS1R) mutations in the population of patients with normosmic congenital hypogonadotrophic hypogonadism (nCHH) by comparison with other genes, involved in gonadotrophin-releasing hormone (GnRH) release or action? KISS1R mutants are responsible for the nCHH phenotype in only a small minority of cases and were less prevalent than GnRH Receptor (GNRHR) mutations. The respective prevalence of each of the genetic causes of nCHH is unclear. Large series of patients are very rare and suffer from heterogeneity of the population of CHH studied. Patients with nCHH were consecutively enrolled in a single French referral centre and were gradually tested for KISS1R between January 2006 and April 2015. A total of 603 patients with nCHH (399 men and 204 women) were diagnosed at the Bicêtre Hospital and underwent KISS1R analysis. The GNRHR, tachykinin receptor 3 (TACR3), gonadotrophin-releasing hormone 1 (GNRH1), tachykinin 3 (TAC3) and KISS1 genes were also sequenced. Functional characterization of KISS1R mutations included a study of signal transduction using a reporter gene (serum response element-luciferase (SRE-Luc) involved in the mitogen-activated protein (MAP) kinase pathway. We detected 15 KISS1R variants (10 novel), in 12 of the 603 patients (2.0%, 95% CI [0.9-3.1]. KISS1R mutations were less prevalent than GNRHR (4.7%) and TACR3 (2.6%) mutations but more prevalent than GNRH1 (1.5%), TAC3 (1.0%) and KISS1 (0%) mutations. KISS1R mutants were present in the biallelic state in 8 of the 12 patients concerned. Among 5 men with biallelic KISS1R mutations, 4 had either micropenis or cryptorchidism. In vitro analysis of the 5 new variants present in the biallelic state (C95W, Y103*, C115W, P176R and A287E) showed a loss of function. The prevalence of TACR3, GNRH1, TAC3 and KISS1 mutations was calculated from a smaller number of nCHH patients than KISS1R and GNRHR. This should prompt caution concerning the reported prevalence

  7. Ictal kissing: occurrence and etiology.

    Science.gov (United States)

    Asadi-Pooya, Ali A; Fletman, Elizabeth W

    2017-02-01

    Ictal kissing is a rare semiological manifestation in patients with epilepsy. We tried to estimate its occurrence and characterize the underlying etiology. We retrospectively reviewed all video-EEG reports from Jefferson Comprehensive Epilepsy Center over a 12-year period (2004-2015) for the occurrence of the term "kiss". We then searched the electronic database PubMed on September 21, 2016 using the following search terms in the English language: "ictal" OR "seizure" OR "epilepsy" AND "kiss" OR "kissing". Relevant original studies were included. During the study period, 5133 patients were investigated in our epilepsy monitoring unit. One patient (0.02%) had one episode of documented ictal kissing. He had drug-resistant right mesial temporal lobe epilepsy. In total, five studies met the criteria for inclusion in the review. These studies described nine patients with drug-resistant focal epilepsy and ictal kissing behavior. Six out of ten patients with ictal kissing had hippocampal sclerosis. We add to the literature on ictal kissing by providing additional information on its frequency and etiology. The most common underlying etiology for ictal kissing is hippocampal sclerosis. However, this semiological ictal phenomenon is not pathognomonic for any etiology or localization.

  8. Different therapeutic effects of distinct KISS1 fragments on breast cancer in vitro and in vivo.

    Science.gov (United States)

    Song, Guo-Qing; Zhao, Yi

    2013-10-01

    Breast cancer is the most frequently diagnosed cancer in women. In these studies, a metastasis suppressor gene, KISS1 and its truncated fragment, were overexpressed in the breast cancer cell line MDA-MB-231. In addition, KISS1 expression was downregulated in MDA-MB-157 cell line using a KISS1-specific siRNA. The effects of KISS1 on breast cancer cells both in vivo and in vitro were then identified. Our results indicate that KISS1 can induce apoptosis and inhibit mobility of breast cancer cells. Moreover, the expression of KISS1 in established xenografted tumors was associated with a decrease in tumor size and weight. Accordingly, the survival rate of these mice was significantly higher compared to that of mice bearing tumors that did not express KISS1. We also confirm that KISS1 could decrease the number of circulating tumor cells (CTCs). The plasma levels of metastin and the number of CTCs were significantly positively correlated. Furthermore, we found that KISS1 can inactivate p-MEK and p-ERK. Overall, these studies demonstrate the antitumor activity of KISS1 in the breast cancer cell lines and provide insight into relevant mechanisms that may lead to novel treatments for breast cancer.

  9. Kissing or "osculation" in frontotemporal dementia.

    Science.gov (United States)

    Mendez, Mario F; Shapira, Jill S

    2014-01-01

    The authors investigated the neuropsychiatry of kissing in frontotemporal dementia. Among 15 patients, two had compulsive social kissing, bitemporal involvement, and Klüver-Bucy symptoms, and four pursued kissing with sexually disinhibited behavior. Future research should clarify the neuropsychiatric significance of kissing behavior.

  10. Developmental Profile and Sexually Dimorphic Expression of Kiss1 and Kiss1r in the Fetal Mouse Brain

    Directory of Open Access Journals (Sweden)

    John Gabriel Knoll

    2013-10-01

    Full Text Available The hypothalamic-pituitary-gonadal axis (HPG is a complex neuroendocrine circuit involving multiple levels of regulation. Kisspeptin neurons play essential roles in controlling the HPG axis from the perspectives of puberty onset, oscillations of gonadotropin releasing hormone (GnRH neuron activity and the pre-ovulatory LH surge. The current studies focus on the expression of kisspeptin during murine fetal development using in situ hybridization (ISH, quantitative reverse transcription real-time PCR (QPCR and immunocytochemistry. Expression of mRNA coding for kisspeptin (KISS1 and its receptor KISS1R was observed at embryonic (E day 13 by ISH. At E13 and other later ages examined, Kiss1 signal in individual cells within the arcuate nucleus (ARC appeared stronger in females than males. ISH examination of agonadal steroidogenic factor-1 (Sf1 knockout mice revealed that E17 XY knockouts resembled wild-type XX females. These findings raise the possibility that gonadal hormones modulate the expression of Kiss1 in the ARC prior to birth. The sex and genotype differences were tested quantitatively by QPCR experiments in dissected hypothalami from mice at E17 and adulthood. Females had significantly more Kiss1 than males at both ages, even though the number of cells detected by ISH was similar. In addition, QPCR revealed a significant difference in the amount of Kiss1 mRNA in Sf1 mice with wild-type (WT XY mice expressing less than XY knockouts (KO and XX mice of both genotypes. The detection of immunoreactive KISS1 in perikarya of the ARC at E17 indicates that early mRNA is translated to peptide. The functional significance of this early expression of Kiss1 awaits elucidation.

  11. Kissing nevus of the penis.

    Science.gov (United States)

    Yun, Sook Jung; Wi, Hyun Seung; Lee, Jee-Bum; Kim, Seong-Jin; Won, Young Ho; Lee, Seung-Chul

    2011-11-01

    Kissing or divided nevi are similar in shape to congenital melanocytic nevi located on an adjacent part of the body that are separated during embryogenesis. Kissing nevi of the upper and lower eyelids have been reported infrequently since the first report in 1908. Kissing nevi of the penis are very rare, with only 12 cases being reported until now, and this is the first case report in the Korean dermatological literature. A previously healthy 27-year-old man presented with asymptomatic black colored patches, which were detected 10 years ago, on the glans penis and the prepuce with growth in size. We report here a case of kissing nevus of the penis, which showed an obvious mirror-image symmetry relative to the coronal sulcus.

  12. Students' Attitudes toward an After-School Physical Activity Programme

    Science.gov (United States)

    Agbuga, Bulent; Xiang, Ping; McBride, Ron

    2013-01-01

    Though considerable research on student attitudes has been conducted in physical education, little information exists concerning student attitudes toward after-school physical activity programmes. This study assessed students' attitudes toward their after-school physical activity programme located in southwest Texas, USA. Participants included 158…

  13. Stated Uptake of Physical Activity Rewards Programmes Among Active and Insufficiently Active Full-Time Employees.

    Science.gov (United States)

    Ozdemir, Semra; Bilger, Marcel; Finkelstein, Eric A

    2017-04-22

    Employers are increasingly relying on rewards programmes in an effort to promote greater levels of activity among employees; however, if enrolment in these programmes is dominated by active employees, then they are unlikely to be a good use of resources. This study uses a stated-preference survey to better understand who participates in rewards-based physical activity programmes, and to quantify stated uptake by active and insufficiently active employees. The survey was fielded to a national sample of 950 full-time employees in Singapore between 2012 and 2013. Participants were asked to choose between hypothetical rewards programmes that varied along key dimensions and whether or not they would join their preferred programme if given the opportunity. A mixed logit model was used to analyse the data and estimate predicted uptake for specific programmes. We then simulated employer payments based on predictions for the percentage of each type of employee likely to meet the activity goal. Stated uptake ranged from 31 to 67% of employees, depending on programme features. For each programme, approximately two-thirds of those likely to enrol were insufficiently active. Results showed that insufficiently active employees, who represent the majority, are attracted to rewards-based physical activity programmes, and at approximately the same rate as active employees, even when enrolment fees are required. This suggests that a programme with generous rewards and a modest enrolment fee may have strong employee support and be within the range of what employers may be willing to spend.

  14. Costs and benefits of Danish active labour market programmes

    DEFF Research Database (Denmark)

    Jespersen, Svend; Munch, Jakob Roland; Skipper, Lars

    2008-01-01

    Since 1994, unemployed workers in the Danish labour market have participated in active labour market programmes on a large scale. This paper contributes with an assessment of costs and benefits of these programmes. Long-term treatment effects are estimated on a very detailed administrative dataset...

  15. Active Labour Market Programme Participation for Unemployment Insurance Recipients

    DEFF Research Database (Denmark)

    Filges, Trine; Smedslund, Geir; Jørgensen, Anne-Marie Klint

    2016-01-01

    Objective: This review evaluates the effectiveness of Active Labour Market Programme (ALMP) participation on employment status for unemployment insurance recipients. Methods and Analysis: We followed Campbell Collaboration guidelines to conduct a systematic review. Results: A total of 73 studies...

  16. Diversity Programme Activity Report 2012-2015

    CERN Document Server

    Guinot, Genevieve; CERN. Geneva. HR Department

    2016-01-01

    CERN’s Diversity programme was launched in 2012 and is now firmly established as one of the pillars of the Organization’s Human Resources strategy. This report provides information on the actions undertaken in pursuit of the seven diversity strategic objectives set for the period from 2012 to 2015 in the priority areas identified for actions of recruitment, career development and work environment. The report highlights the progress made in anchoring the diversity principles in policies, procedures and practices within the Organization, as well as the persistent difficulties for the Organization to improve the representation of some nationalities and to attract female applicants in science and engineering. It shows a constant increase of female representation in managerial positions and encouraging results in building a diverse talent pool of scientists and engineers through the various student and graduate programmes. The report also outlines how the Diversity Office contributed through several actions,...

  17. Pseudoperforation during kissing balloon angioplasty.

    Science.gov (United States)

    Panetta, Carmelo J; Fasseas, Panayotis; Raveendran, Ganesh; Garratt, Kirk N

    2004-11-01

    We describe a case of apparent perforation during kissing balloon angioplasty of a bifurcation lesion. There was no evidence of perforation on follow-up angiography or via intravascular ultrasound. Possible etiologies include minimal perforation that immediately sealed postdeflation or forced contrast into the microvascular bed via a proximal side branch.

  18. Lid reconstruction for kissing naevus

    Directory of Open Access Journals (Sweden)

    Betharia S

    1988-01-01

    Full Text Available Repair of colobomas caused after excision of kissing naevi presents a special problem because of their symmetrical placement. Successful repair in two cases with the surgical-technique is presented. Canthotomy and cantholysis along with direct suturing and Tenzel′s semicircular rotation flap gave excellent results.

  19. The right way to kiss: directionality bias in head-turning during kissing.

    Science.gov (United States)

    Karim, A K M Rezaul; Proulx, Michael J; de Sousa, Alexandra A; Karmaker, Chhanda; Rahman, Arifa; Karim, Fahria; Nigar, Naima

    2017-07-14

    Humans have a bias for turning to the right in a number of settings. Here we document a bias in head-turning to the right in adult humans, as tested in the act of kissing. We investigated head-turning bias in both kiss initiators and kiss recipients for lip kissing, and took into consideration differences due to sex and handedness, in 48 Bangladeshi heterosexual married couples. We report a significant male bias in the initiation of kissing and a significant bias in head-turning to the right in both kiss initiators and kiss recipients, with a tendency among kiss recipients to match their partners' head-turning direction. These interesting outcomes are explained by the influences of societal learning or cultural norms and the potential neurophysiological underpinnings which together offer novel insights about the mechanisms underlying behavioral laterality in humans.

  20. Molecular Properties of Kiss1 Neurons in the Arcuate Nucleus of the Mouse

    Science.gov (United States)

    Gottsch, Michelle L.; Popa, Simina M.; Lawhorn, Janessa K.; Qiu, Jian; Tonsfeldt, Karen J.; Bosch, Martha A.; Kelly, Martin J.; Rønnekleiv, Oline K.; Sanz, Elisenda; McKnight, G. Stanley; Clifton, Donald K.; Palmiter, Richard D.

    2011-01-01

    Neurons that produce kisspeptin play a critical role in reproduction. However, understanding the molecular physiology of kisspeptin neurons has been limited by the lack of an in vivo marker for those cells. Here, we report the development of a Kiss1-CreGFP knockin mouse, wherein the endogenous Kiss1 promoter directs the expression of a Cre recombinase-enhanced green fluorescent protein (GFP) fusion protein. The pattern of GFP expression in the brain of the knockin recapitulates what has been described earlier for Kiss1 in the male and female mouse, with prominent expression in the arcuate nucleus (ARC) (in both sexes) and the anteroventral periventricular nucleus (in females). Single-cell RT-PCR showed that the Kiss1 transcript is expressed in 100% of GFP-labeled cells, and the CreGFP transcript was regulated by estradiol in the same manner as the Kiss1 gene (i.e. inhibited in the ARC and induced in the anteroventral periventricular nucleus). We used this mouse to evaluate the biophysical properties of kisspeptin (Kiss1) neurons in the ARC of the female mouse. GFP-expressing Kiss1 neurons were identified in hypothalamic slice preparations of the ARC and patch clamped. Whole-cell (and loose attached) recordings revealed that Kiss1 neurons exhibit spontaneous activity and expressed both h- (pacemaker) and T-type calcium currents, and hyperpolarization-activated cyclic nucleotide-regulated 1–4 and CaV3.1 channel subtypes (measured by single cell RT-PCR), respectively. N-methyl-D-aspartate induced bursting activity, characterized by depolarizing/hyperpolarizing oscillations. Therefore, Kiss1 neurons in the ARC share molecular and electrophysiological properties of other CNS pacemaker neurons. PMID:21933870

  1. PROGRAMME

    African Journals Online (AJOL)

    MEASUREMENT OF SURVIVAL IN A PRIVATE. SECTOR HIV/AIDS DISEASE ... during the first 4 years of the programme was published in 2003.' The purpose of this ... that their scheme offers a Dl\\/IP, and must be willing to disclose their HlV ...

  2. KISS: Discovery and Spectroscopic Classification of a Type Ia Supernova KISS15q

    Science.gov (United States)

    Morokuma, Tomoki; Tominaga, Nozomu; Tanaka, Masaomi; Jiang, Ji-an; Shibata, Takumi; Kokubo, Mitsuru; Hashiba, Yasuhito; Mitsuda, Kazuma; Doi, Mamoru; Sako, Shigeyuki; Kikuchi, Yuki; Takahashi, Hidenori; Tateuchi, Ken; Kuncarayakti, Hanindyo; Watanabe, Makoto; Nakao, Hikaru; Itoh, Yoichi; Morihana, Kumiko; Honda, Satoshi; Takagi, Yuhei; Takahashi, Jun; Takeishi, Masanori

    2015-05-01

    We report the discovery and spectroscopic identification of a Type Ia supernova, KISS15q. In Kiso Supernova Survey (KISS; Morokuma et al. 2014, PASJ, 66, 118), we found a transient object KISS15q of g=20.6 on May 19.60, 2015 UT in the g-band image.

  3. From KISS to Implementation: A Study of Problems, Solutions and Open Issues

    DEFF Research Database (Denmark)

    Valente, Andrea; van Bommel, Patrick

    1995-01-01

    and quite easy to parse, in order to produce code for the system. This means that KFD is really a portable language and that we can improve, optimize, implementation technique in future without involving the KFD programmers, or existing KFD definition of a system. When we create a complete KISS formal...

  4. Digitally Controlled ’ProgrammableActive Filters.

    Science.gov (United States)

    1985-12-01

    Mitra, S. K., Analysis and Synthesis of Linear Active .. Networks, Wiley, New York, 1969. * 6. Sedra , A. S. and Smith , K. C., "A Second-Generation...Current Conveyor and its Applications," IEEE Trans. Circuit Theory, Vol. CT-17, pp. 132-134, 1970. 7. Sedra , A. S., "A New Approach to Active Network...CT-18, pp. 358-361, May 1971. 27. Hamilton, T. A., and Sedra , A. S., "Some New IJ Configurations for Active Filters," IEEE Trans. Circuit Tehory, Vol

  5. Self-assembling RNA nanorings based on RNAI/II inverse kissing complexes

    Science.gov (United States)

    Grabow, Wade W.; Zakrevsky, Paul; Afonin, Kirill A.; Chworos, Arkadiusz; Shapiro, Bruce A.; Jaeger, Luc

    2011-01-01

    RNA is an attractive biopolymer for nanodesign of self-assembling particles for nanobiotechnology and synthetic biology. Here, we experimentally characterize by biochemical and biophysical methods the formation of thermostable and ribonuclease resistant RNA nanorings previously proposed by computational design. High yields of fully programmable nanorings were produced based on several RNAI/IIi kissing complex variants selected for their ability to promote polygon self-assembly. This self-assembly strategy relying on the particular geometry of bended kissing complexes has potential for developing siRNAs delivery agents. PMID:21229999

  6. Primer on CDM programme of activities

    Energy Technology Data Exchange (ETDEWEB)

    Hinostroza, M. (UNEP Risoe Centre, Roskilde (Denmark)); Lescano, A.D. (A2G Carbon Partners (Peru)); Alvarez, J.M. (Ministerio del Ambiente del Peru (Peru)); Avendano, F.M. (EEA Fund Management Ltd. (United Kingdom)

    2009-07-01

    As an advanced modality introduced in 2005, the Programmatic CDM (POA) is expected to address asymmetries of participation, especially of very small-scale project activities in certain areas, key sectors and many countries with considerable potential for greenhouse gas emission reductions, not reached by the traditional single-project-based CDM. Latest experiences with POAs and the recently finalized official guidance governing the Programmatic CDM are the grassroots of this Primer, which has the purpose of supporting the fully understanding of rules and procedures of POAs by interpreting them and analyzing real POA cases. Professional and experts from the public and private entities have contributed to the development of this Primer, produced by the UNEP Risoe Centre, as part of knowledge support activities for the Capacity Development for the CDM (CD4CDM) project. The overall objective of the CD4CDM is to develop the capacities of host countries to identify, design, approve, finance, implement CDM projects and commercialize CERs in participating countries. The CDM4CDM is funded by the Netherlands Ministry of Foreign Affairs. (author)

  7. Yugoslavia. "Migration" -- programme activities targeting men.

    Science.gov (United States)

    Dzeletovic, A; Matovic-miljanovic, S

    1999-01-01

    In Yugoslavia, companies send their workers to different parts of the world, including countries with a high incidence of AIDS. It has been noted that it is characteristic for migrants to accommodate themselves to foreign conditions, which subsequently lead to health problems, especially with regards to reproductive and sexual health. Often, in the case of partner separation, men may seek sexual relations with an unknown partner and/or neglect to use proper protection. According to research carried out in Yugoslavia, there are critical gaps in workers' knowledge on sexual and reproductive health. Based on research results, an educational program for migrants, designed to train and strengthen individuals¿ capabilities and modify their risky behavior, was created. Program activities include production of brochures targeting those people travelling to countries with a high incidence of HIV/AIDS. In addition, a process for creating more cooperation between the state and other organizations at regional and local levels was initiated.

  8. Certain aspects of the psychological analysis of programmer activity. [selection of computer programmers

    Science.gov (United States)

    Bondarovskaya, V. M.

    1975-01-01

    The psychological analysis of programmer activity showed that one of its basic characteristics is the need to employ formal languages. In determining ways of effectively mastering the capacity to write algorithms in the algorithmic language, it is expedient to proceed from its similarity to the living languages and the language of mathematical formulas, and to choose certain psychological principles of mastering foreign languages and mathematical symbols in teaching the algorithmic language. General models of the input language significantly increase the effectiveness of its mastery and permit the development of thinking on the part of the students.

  9. A kiss is not a kiss: visually evoked neuromagnetic fields reveal differential sensitivities to brief presentations of kissing couples.

    Science.gov (United States)

    Cogan, Gregory B; Kirshenbaum, Sheril R; Walker, Jeffrey; Poeppel, David

    2015-09-30

    With a few exceptions, the literature on face recognition and its neural basis derives from the presentation of single faces. However, in many ecologically typical situations, we see more than one face, in different communicative contexts. One of the principal ways in which we interact using our faces is kissing. Although there is no obvious taxonomy of kissing, we kiss in various interpersonal situations (greeting, ceremony, sex), with different goals and partners. Here, we assess the visual cortical responses elicited by viewing different couples kissing with different intents. The study thus lies at the nexus of face recognition, action recognition, and social neuroscience. Magnetoencephalography data were recorded from nine participants in a passive viewing paradigm. We presented images of couples kissing, with the images differing along two dimensions, kiss type and couple type. We quantified event-related field amplitudes and latencies. In each participant, the canonical sequence of event-related fields was observed, including an M100, an M170, and a later M400 response. The earliest two responses were significantly modulated in latency (M100) or amplitude (M170) by the sex composition of the images (with male-male and female-female pairings yielding faster latency M100 and larger amplitude M170 responses). In contrast, kiss type showed no modulation of any brain response. The early cortical-evoked fields that we typically associate with the presentation and analysis of single faces are differentially sensitive to complex social and action information in face pairs that are kissing. The early responses, typically associated with perceptual analysis, exhibit a consistent grouping and suggest a high and rapid sensitivity to the composition of the kissing pairs.

  10. Shaping the oral microbiota through intimate kissing.

    Science.gov (United States)

    Kort, Remco; Caspers, Martien; van de Graaf, Astrid; van Egmond, Wim; Keijser, Bart; Roeselers, Guus

    2014-01-01

    The variation of microbial communities associated with the human body can be the cause of many factors, including the human genetic makeup, diet, age, surroundings, and sexual behavior. In this study, we investigated the effects of intimate kissing on the oral microbiota of 21 couples by self-administered questionnaires about their past kissing behavior and by the evaluation of tongue and salivary microbiota samples in a controlled kissing experiment. In addition, we quantified the number of bacteria exchanged during intimate kissing by the use of marker bacteria introduced through the intake of a probiotic yoghurt drink by one of the partners prior to a second intimate kiss. Similarity indices of microbial communities show that average partners have a more similar oral microbiota composition compared to unrelated individuals, with by far most pronounced similarity for communities associated with the tongue surface. An intimate kiss did not lead to a significant additional increase of the average similarity of the oral microbiota between partners. However, clear correlations were observed between the similarity indices of the salivary microbiota of couples and self-reported kiss frequencies, and the reported time passed after the latest kiss. In control experiments for bacterial transfer, we identified the probiotic Lactobacillus and Bifidobacterium marker bacteria in most kiss receivers, corresponding to an average total bacterial transfer of 80 million bacteria per intimate kiss of 10 s. This study indicates that a shared salivary microbiota requires a frequent and recent bacterial exchange and is therefore most pronounced in couples with relatively high intimate kiss frequencies. The microbiota on the dorsal surface of the tongue is more similar among partners than unrelated individuals, but its similarity does not clearly correlate to kissing behavior, suggesting an important role for specific selection mechanisms resulting from a shared lifestyle, environment

  11. Kissing in the Shadow

    Directory of Open Access Journals (Sweden)

    Paul Thomas

    2013-05-01

    Full Text Available 28 August, Newtown, Sydney: following an encounter the day prior at the National Institute for Experimental Arts, they meet again. A fluid discussion around art, perception, philosophy, vision, probabilities, and physics resulted—transforming into an activity of mobile phone photography—a photo-walk, a means of engaging with the world. The walk down King Street is a visual discussion, collected observations of things noted, a probability map, and a becoming, “like the emergence of another world.”

  12. Differential regulation of kiss1 expression by melatonin and gonadal hormones in male and female Syrian hamsters

    DEFF Research Database (Denmark)

    Ansel, L; Bolborea, M; Bentsen, A H;

    2010-01-01

    In seasonal breeders, reproduction is synchronized to seasons by day length via the pineal hormone melatonin. Recently, we have demonstrated that Kiss1, a key activator of the reproductive function, is down-regulated in sexually inactive hamsters maintained in inhibitory short days (SDs). In rode......In seasonal breeders, reproduction is synchronized to seasons by day length via the pineal hormone melatonin. Recently, we have demonstrated that Kiss1, a key activator of the reproductive function, is down-regulated in sexually inactive hamsters maintained in inhibitory short days (SDs...... differentially regulate Kiss1 expression in the ARC and the AVPV. Kiss1 expression was examined by in situ hybridization in both male and female hamsters kept in various experimental conditions, and we observed that 1) SD exposure markedly reduced Kiss1 expression in the ARC and AVPV of male and female hamsters...... as compared to LD animals, 2) sex steroid treatment in SD-adapted male and female hamsters increased the number of Kiss1 neurons in the AVPV but decreased it in the ARC, 3) melatonin administration to LD-adapted hamsters decreased Kiss1 mRNA level in both the AVPV and the ARC in intact animals, whereas...

  13. Delimiting Maximal Kissing Configurations in Four Dimensions

    CERN Document Server

    Altschuler, Eric Lewin

    2013-01-01

    How many unit $n-$dimensional spheres can simultaneously touch or kiss a central $n-$dimensional unit sphere? Beyond mathematics this question has implications for fields such as cryptography and the structure of biologic and chemical macromolecules. The kissing number is only known for dimensions 1-4, 8 and 24 (2, 6, 12, 24, 240, 19650, respectively) and only particularly obvious for dimensions one and two. Indeed, in four dimensions it is not even known if Platonic polytope unique to that dimension known as the 24-cell is the unique kissing configuration. We have not been able to prove that the 24-cell is unique, but, using a physical approach utilizing the hopf map from four to three dimensions, we for the first time delimit the possible other configurations which could be kissing in four dimensions.

  14. Ictal kissing behavior: neurological and psychodynamic overview.

    Science.gov (United States)

    Taşkıran, Emine; Özmen, Mine; Kılıc, Özge; Sentürk, Aslı; Özkara, Cigdem

    2013-11-01

    This study is based on the psychodynamic and neurological analysis of three Turkish patients who displayed ictal kissing automatism during their seizures. To unveil the probable underlying causes of their kissing behaviors, all patients underwent psychiatric interviews after being evaluated by ictal video-EEG recordings. The group consisted of two females (ages 35 and 29) and one male (age 26). In addition to prominent oral automatisms, each patient also displayed behaviors of kissing or blowing kisses to individuals at close proximity. Seizures were related to the right temporal lobe in two patients and the left temporal lobe in one patient. Magnetic resonance imaging showed mesial temporal sclerosis in two of the patients (one left, one right) and was normal in one. According to the DSM-IV-TR criteria, each of the three patients also suffered from major depression, while the psychodynamic interviews revealed traumatic childhood histories and intense unfulfilled affective needs. © 2013.

  15. Issues related to a programme of activities under the CDM

    Energy Technology Data Exchange (ETDEWEB)

    Ellis, J.

    2006-05-15

    Emissions of CO2 from the energy and land-use change and forestry sectors are responsible for the majority of emissions in non-Annex I Parties to the UNFCCC. Tackling greenhouse gas (GHG) emissions from these sectors is a key to slowing the growth in GHG emissions in non-Annex I countries. Implementing Clean Development Mechanism (CDM) projects can help achieve this aim, while also assisting non-Annex I countries to move towards sustainable development and Annex I countries achieve their emission commitments under the Kyoto Protocol. There has been rapid progress in the CDM over the last year - in terms of the number of projects in the pipeline and registered, and in terms of credits issued. However, some important sectors are notable by their small share in the CDM portfolio. Several countries have also called attention to the need to accelerate the process of approving CDM methodologies and projects. In order to improve the effectiveness of the CDM to achieve its dual objectives, the COP/MOP agreed a decision on 'further guidance relating to the clean development mechanism. This decision lays out guidance on how to improve the operation of the CDM, and includes provisions that allow: (1) Bundling of project activities; and (2) Project activities under a programme of activities, to be registered as a CDM project activity. At present, of the 172 currently registered CDM project activities, 27 involve programmes or bundles. These project activities can include more than one project type, be implemented in several locations, and/or occur in more than one sector. This paper assesses how project activities under a programme of activities under the CDM (referred to here as PCDM) could help to increase the effectiveness of the CDM by encouraging a wide spread of emission mitigation activities. This paper also explores the key issues that may need to be considered for the PCDM concept to be further implemented. The paper concludes that: (1) Key concepts and issues

  16. Baastrup's disease: The kissing spine.

    Science.gov (United States)

    Singla, Amit; Shankar, Vivek; Mittal, Samarth; Agarwal, Abhinav; Garg, Bhavuk

    2014-02-16

    A 67-year-old male presented with a gradually progressive low back pain of 2 years duration. The patient was leading a retired life and there was no history of chronic fever or significant trauma. There was no radiation of pain or any features suggestive of claudication. There was no history of any comorbidity. The pain was aggravated with extension of the spine and relieved with flexion. There was no swelling or neurological deficit, but muscle spasm was present. Radiographs of the spine revealed degenerative changes in the lumbosacral spine, along with articulation of spinous processes at in lumbar spine at all levels level suggestive of Baastrup's disease, commonly known as "kissing spine". Routine blood investigations were within normal limits. The patient was managed conservatively. He was given a week's course of analgesics and muscle relaxants and then started on spinal flexion exercises, with significant improvement being noted at 6 months follow up.

  17. Active Labour Market Programme Participation for Unemployment Insurance Recipients

    DEFF Research Database (Denmark)

    Filges, Trine; Smedslund, Geir; Jørgensen, Anne-Marie Klint

    2016-01-01

    Objective: This review evaluates the effectiveness of Active Labour Market Programme (ALMP) participation on employment status for unemployment insurance recipients. Methods and Analysis: We followed Campbell Collaboration guidelines to conduct a systematic review. Results: A total of 73 studies...... met the inclusion criteria and were critically appraised by the review authors. The available evidence suggests that there is a general effect of participating in ALMP. The findings are mixed, however, depending on the approach used to investigate the effect, with no effect found of being assigned...

  18. To kiss or not to kiss? Impact of final kissing-balloon inflation on early and long-term results of percutaneous coronary intervention for bifurcation lesions.

    Science.gov (United States)

    Biondi-Zoccai, Giuseppe; Sheiban, Imad; De Servi, Stefano; Tamburino, Corrado; Sangiorgi, Giuseppe; Romagnoli, Enrico

    2014-11-01

    Final kissing-balloon inflation is often recommended for percutaneous coronary intervention (PCI) of bifurcation lesions. However, randomized trials focusing on kissing inflation have not confirmed its beneficial impact. We compared outcomes of kissing inflation for PCI of bifurcation lesions, explicitly stratifying results according to stenting strategy. Patients undergoing bifurcation PCI were retrospectively enrolled. Subjects receiving final kissing inflation were compared with those not undergoing kissing inflation, after stratification for a single-stent technique. The primary end point was the long-term rate of major adverse cardiac events (MACE, i.e., death, myocardial infarction, or target lesion revascularization (TLR)). A total of 4314 patients were included: 1176 (27.3 %) treated with a single stent and kissing inflation, 1637 (37.9 %) with a single stent but no kissing, 1072 (24.8 %) with two stents and kissing, and 429 (9.9 %) with two stents but no kissing. At unadjusted analyses kissing was associated with fewer short-term MACE and deaths in the two-stent group, and with fewer long-term MACE, cardiac deaths, and side-branch TLR in the two-stent group (all P kissing appeared detrimental after single stenting. However, after multivariable analyses, kissing no longer significantly affected the risk of adverse events, with the exception of the risk of side-branch TLR, which was lower in those receiving two stents and final kissing inflation (hazard ratio = 0.52, 95 % confidence interval 0.30–0.90, P = 0.020). Kissing inflation can be avoided in bifurcation lesions uneventfully treated with single-stent PCI. However, final kissing-balloon inflation appears beneficial in reducing the risk of side-branch repeat revascularization after using a two-stent strategy.

  19. Hyper-brain networks support romantic kissing in humans.

    Science.gov (United States)

    Müller, Viktor; Lindenberger, Ulman

    2014-01-01

    Coordinated social interaction is associated with, and presumably dependent on, oscillatory couplings within and between brains, which, in turn, consist of an interplay across different frequencies. Here, we introduce a method of network construction based on the cross-frequency coupling (CFC) and examine whether coordinated social interaction is associated with CFC within and between brains. Specifically, we compare the electroencephalograms (EEG) of 15 heterosexual couples during romantic kissing to kissing one's own hand, and to kissing one another while performing silent arithmetic. Using graph-theory methods, we identify theta-alpha hyper-brain networks, with alpha serving a cleaving or pacemaker function. Network strengths were higher and characteristic path lengths shorter when individuals were kissing each other than when they were kissing their own hand. In both partner-oriented kissing conditions, greater strength and shorter path length for 5-Hz oscillation nodes correlated reliably with greater partner-oriented kissing satisfaction. This correlation was especially strong for inter-brain connections in both partner-oriented kissing conditions but not during kissing one's own hand. Kissing quality assessed after the kissing with silent arithmetic correlated reliably with intra-brain strength of 10-Hz oscillation nodes during both romantic kissing and kissing with silent arithmetic. We conclude that hyper-brain networks based on CFC may capture neural mechanisms that support interpersonally coordinated voluntary action and bonding behavior.

  20. Active ingredients in anti-stigma programmes in mental health.

    Science.gov (United States)

    Pinfold, Vanessa; Thornicroft, Graham; Huxley, Peter; Farmer, Paul

    2005-04-01

    This paper draws upon a review of the relevant literature and the results of the recent Mental Health Awareness in Action (MHAA) programme in England to discuss the current evidence base on the active ingredients in effective anti-stigma interventions in mental health. The MHAA Programme delivered educational interventions to 109 police officers, 78 adults from different community groups whose working lives involved supporting people with mental health problems but who had received no mental health training and 472 schools students aged 14-15. Each adult target group received two intervention sessions lasting two hours. The two school lessons were 50 minutes each. Knowledge, attitudes and behavioural intent were assessed at baseline and follow-up. In addition focus groups were held with mental health service users to explore the impact of stigma on their lives and facilitators of educational workshops were interviewed to provide expert opinion on 'what works' to reduce psychiatric stigma. Personal contact was predictive of positive changes in knowledge and attitudes for the school students but not the police officers or community adult group. The key active ingredient identified by all intervention groups and workshop facilitators were the testimonies of service users. The statements of service users (consumers) about their experience of mental health problems and of their contact with a range of services had the greatest and most lasting impact on the target audiences in terms of reducing mental health stigma.

  1. Study of kissing molars in Turkish population sample | Yanik ...

    African Journals Online (AJOL)

    Study of kissing molars in Turkish population sample. ... refers to contacting occlusal surfaces of the impacted mandibular second and third molars. ... was to report the incidence of kissing molars (KMs), classification, incorporated pathologies, ...

  2. An inexpensive programmable illumination microscope with active feedback

    Science.gov (United States)

    Tompkins, Nathan; Fraden, Seth

    2016-02-01

    We have developed a programmable illumination system capable of tracking and illuminating numerous objects simultaneously using only low-cost and reused optical components. The active feedback control software allows for a closed-loop system that tracks and perturbs objects of interest automatically. Our system uses a static stage where the objects of interest are tracked computationally as they move across the field of view allowing for a large number of simultaneous experiments. An algorithmically determined illumination pattern can be applied anywhere in the field of view with simultaneous imaging and perturbation using different colors of light to enable spatially and temporally structured illumination. Our system consists of a consumer projector, camera, 35-mm camera lens, and a small number of other optical and scaffolding components. The entire apparatus can be assembled for under 4,000. Supplemental matlab code is available to assist in the setup of the active feedback software.

  3. Ictal kissing with subdural EEG recording.

    Science.gov (United States)

    Alsemari, Abdulaziz; Alotaibi, Faisal; Baz, Salah

    2013-01-01

    Ictal kissing has been described in the literature. Five cases were reported and associated with temporal lobe epilepsy lateralizing to the nondominant hemisphere. A case of ictal kissing was identified. The aim was to demonstrate the clinical, clinical and electrophysiological features (as recorded by subdural electrodes). The surgical procedure, histopathology, and imaging data were reviewed and correlated with the literature. A 29-year-old right-handed female, who presented with ictal right hand left arm dystonic posturing, and lip smacking, was studied. The automatism was usually followed by prolonged emotional gestures and by hugging and kissing her relative and/or attendant nurse. Magnetic resonance imaging of the brain showed right small cortical and subcortical lesions of the right inferior frontal lobe with gliosis but without mass effect and normal-sized hippocampi. The PET scan showed hypometabolism of the right temporal lobe. Neuropsychological evaluation showed deficit in her nonverbal memory. The subdural electrodes showed high amplitude spikes over right mesial temporal lobe strips. The offsite of the ictal discharges was usually at the right frontal strips. Right standard temporal lobectomy with amygdalohippocampectomy and right inferior frontal lesionectomy were performed. The patient continued to be seizure-free for one year postoperatively. Our case report supports with subdural EEG recording the findings of the few reported cases of ictal kissing behavior lateralized to the nondominant hemisphere. However, the affectionate kissing behavior was associated with spread of the epileptic discharges to the right frontal lobe.

  4. Ictal kissing: a release phenomenon in non-dominant temporal lobe epilepsy.

    Science.gov (United States)

    Rashid, Razi M; Eder, Katherine; Rosenow, Joshua; Macken, Michael P; Schuele, Stephan U

    2010-12-01

    A variety of ictal automatisms with strong emotional elements have been described. Ictal kissing has not been well characterized and may provide useful clinical information and insight into the mechanisms of stereotyped ictal behaviour. Three of 220 patients (1.4%) admitted for video EEG monitoring between 7/2006 and 6/2009 with ictal kissing were identified. Clinical, neurophysiological and imaging data were reviewed and correlated. All patients were right-handed women with a longstanding history and findings consistent with right temporal lobe epilepsy (TLE). Multiple habitual events were recorded for each patient, characterized by staring, oral automatisms and amnesia. In addition, partial preservation of responsiveness and speech were seen during seizures in all three patients. The first two patients showed kissing behaviour triggered by interactions during seizure testing. The last patient had six seizures overnight and developed spontaneous kissing behaviour in the ictal and post-ictal period of her later events, associated with hyperorality and sexual disinhibition. Our series supports the findings of two prior cases, of one male and one female, which reported IK behaviour associated with TLE and lateralizing to the non-dominant hemisphere. This behaviour has not been described in simple partial seizures or seen with electrical cortical stimulation suggesting that it represents a release phenomenon rather than activation of distinct symptomatogenic cortex. This is also supported by the fact that ictal kissing can occur within the spectrum of seizure-induced Kluver-Bucy syndrome and may extend into the post-ictal period.

  5. An inexpensive programmable illumination microscope with active feedback

    Science.gov (United States)

    Tompkins, Nathan; Fraden, Seth

    2016-01-01

    We have developed a programmable illumination system capable of tracking and illuminating numerous objects simultaneously using only low-cost and reused optical components. The active feedback control software allows for a closed-loop system that tracks and perturbs objects of interest automatically. Our system uses a static stage where the objects of interest are tracked computationally as they move across the field of view allowing for a large number of simultaneous experiments. An algorithmically determined illumination pattern can be applied anywhere in the field of view with simultaneous imaging and perturbation using different colors of light to enable spatially and temporally structured illumination. Our system consists of a consumer projector, camera, 35-mm camera lens, and a small number of other optical and scaffolding components. The entire apparatus can be assembled for under $4,000. PMID:27642182

  6. Examining the possible functions of kissing in romantic relationships.

    Science.gov (United States)

    Wlodarski, Rafael; Dunbar, Robin I M

    2013-11-01

    Recent research suggests that romantic kissing may be utilized in human sexual relationships to evaluate aspects of a potential mate's suitability, to mediate feelings of attachment between pair-bonded individuals, or to facilitate arousal and initiate sexual relations. This study explored these potential functions of romantic kissing by examining attitudes towards the importance of kissing in the context of various human mating situations. The study involved an international online questionnaire, which was completed by 308 male and 594 female participants aged 18-63 years. Support was found for the hypothesis that kissing serves a useful mate-assessment function: women, high mate-value participants, and participants high in sociosexual orientation placed greater importance on kissing in romantic relationships and stated that an initial kiss was more likely to affect their attraction to a potential mate than did men, low-mate value participants or low sociosexual orientation participants. Kissing also seemed to be utilized in the mediation of pair-bond attachments: kissing was seen to be more important at established stages of relationships by low sociosexual participants, kissing was generally seen as more important in long-term relationship contexts (but particularly so by women), and kissing frequency was found to be related to relationship satisfaction. The findings of this research showed very little evidence to support the hypothesis that the primary function of kissing is to elevate levels of arousal.

  7. A remarkably stable kissing-loop interaction defines substrate recognition by the Neurospora Varkud Satellite ribozyme.

    Science.gov (United States)

    Bouchard, Patricia; Legault, Pascale

    2014-09-01

    Kissing loops are tertiary structure elements that often play key roles in functional RNAs. In the Neurospora VS ribozyme, a kissing-loop interaction between the stem-loop I (SLI) substrate and stem-loop V (SLV) of the catalytic domain is known to play an important role in substrate recognition. In addition, this I/V kissing-loop interaction is associated with a helix shift in SLI that activates the substrate for catalysis. To better understand the role of this kissing-loop interaction in substrate recognition and activation by the VS ribozyme, we performed a thermodynamic characterization by isothermal titration calorimetry using isolated SLI and SLV stem-loops. We demonstrate that preshifted SLI variants have higher affinity for SLV than shiftable SLI variants, with an energetic cost of 1.8-3 kcal/mol for the helix shift in SLI. The affinity of the preshifted SLI for SLV is remarkably high, the interaction being more stable by 7-8 kcal/mol than predicted for a comparable duplex containing three Watson-Crick base pairs. The structural basis of this remarkable stability is discussed in light of previous NMR studies. Comparative thermodynamic studies reveal that kissing-loop complexes containing 6-7 Watson-Crick base pairs are as stable as predicted from comparable RNA duplexes; however, those with 2-3 Watson-Crick base pairs are more stable than predicted. Interestingly, the stability of SLI/ribozyme complexes is similar to that of SLI/SLV complexes. Thus, the I/V kissing loop interaction represents the predominant energetic contribution to substrate recognition by the trans-cleaving VS ribozyme. © 2014 Bouchard and Legault; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

  8. What's in a Kiss? The Effect of Romantic Kissing on Mate Desirability

    Directory of Open Access Journals (Sweden)

    Rafael Wlodarski

    2014-01-01

    Full Text Available Past research suggests that various courtship rituals, such as romantic kissing, may convey useful mate quality information. Two studies were carried out to examine how purported romantic kissing abilities, as a potential cue to some form of mate information, affect appraisals of potential mating partners. In Experiment 1, 724 participants were presented with vignette descriptions of potential mating partners and were asked to rate partner desirability for various mating-related situations. The primary result of this experiment was that purported kissing ability increased mate desirability in “casual sex” mating situations for women to a greater extent than for men. Experiment 2 repeated the same procedure with another 178 participants, this time including visual information alongside vignette descriptions containing kissing-related information to examine the relative effects of these two modalities. It was found that the presence of a picture alongside a descriptive vignette negated the effect of kissing-related information only when rating potential partners on attractiveness or desirability for further courtship, though not when evaluating partners for casual sex or long-term relationship scenarios. Visual information containing “attractive” photos of potential partners was also found to have a greater effect on men's ratings of partner desirability than on women's ratings of partner desirability. The results are discussed in light of romantic kissing's potential function of conveying important mate quality and desirability information, and its relative role in the presence of additional visual mate cues.

  9. What's in a kiss? The effect of romantic kissing on mating desirability.

    Science.gov (United States)

    Wlodarski, Rafael; Dunbar, Robin I M

    2014-03-19

    Past research suggests that various courtship rituals, such as romantic kissing, may convey useful mate quality information. Two studies were carried out to examine how purported romantic kissing abilities, as a potential cue to some form of mate information, affect appraisals of potential mating partners. In Experiment 1, 724 participants were presented with vignette descriptions of potential mating partners and were asked to rate partner desirability for various mating-related situations. The primary result of this experiment was that purported kissing ability increased mate desirability in "casual sex" mating situations for women to a greater extent than for men. Experiment 2 repeated the same procedure with another 178 participants, this time including visual information alongside vignette descriptions containing kissing-related information to examine the relative effects of these two modalities. It was found that the presence of a picture alongside a descriptive vignette negated the effect of kissing-related information only when rating potential partners on attractiveness or desirability for further courtship, though not when evaluating partners for casual sex or long-term relationship scenarios. Visual information containing "attractive" photos of potential partners was also found to have a greater effect on men's ratings of partner desirability than on women's ratings of partner desirability. The results are discussed in light of romantic kissing's potential function of conveying important mate quality and desirability information, and its relative role in the presence of additional visual mate cues.

  10. Programme Costing of a Physical Activity Programme in Primary Prevention: Should the Costs of Health Asset Assessment and Participatory Programme Development Count?

    Directory of Open Access Journals (Sweden)

    Silke B. Wolfenstetter

    2012-01-01

    Full Text Available This analysis aims to discuss the implications of the “health asset concept”, introduced by the WHO, and the “investment for health model” requiring a “participatory approach” of cooperative programme development applied on a physical activity programme for socially disadvantaged women and to demonstrate the related costing issues as well as the relevant decision context. The costs of programme implementation amounted to €48,700. Adding the costs for developing the programme design of €48,800 results in total costs of €97,500; adding on top of that the costs of asset assessment running to €35,600 would total €133,100. These four different cost figures match four different types of potentially relevant decisions contexts. Depending on the decision context the total costs, and hence the incremental cost-effectiveness ratio of a health promotion intervention, could differ considerably. Therefore, a detailed cost assessment and the identification of the decision context are of crucial importance.

  11. Automatic segmentation of coronary arteries in CT imaging in the presence of kissing vessel artifacts.

    Science.gov (United States)

    Wang, Yin; Liatsis, Panos

    2012-07-01

    In this paper, we present a novel two-step algorithm for segmentation of coronary arteries in computed tomography images based on the framework of active contours. In the proposed method, both global and local intensity information is utilized in the energy calculation. The global term is defined as a normalized cumulative distribution function, which contributes to the overall active contour energy in an adaptive fashion based on image histograms, to deform the active contour away from local stationary points. Possible outliers, such as kissing vessel artifacts, are removed in the postprocessing stage by a slice-by-slice correction scheme based on multiregion competition, where both arteries and kissing vessels are identified and tracked through the slices. The efficiency and the accuracy of the proposed technique are demonstrated on both synthetic and real datasets. The results on clinical datasets show that the method is able to extract the major branches of arteries with an average distance of 0.73 voxels to the manually delineated ground truth data. In the presence of kissing vessel artifacts, the outer surface of the entire coronary tree, extracted by the proposed algorithm, is smooth and contains fewer erroneous regions, originating in kissing vessel artifacts, as compared to the initial segmentation.

  12. Electricity research programme - Overview report on activities in 2005; Programm Elektrizitaet. Ueberblicksbericht zum Forschungsprogramm 2005

    Energy Technology Data Exchange (ETDEWEB)

    Brueniger, R.

    2006-07-01

    This overview-report for the Swiss Federal Office of Energy (SFOE) summarises the work done in 2005 in the various research areas covered by the Swiss Electricity Research programme. Work done in the programme's two main areas - technologies and efficient applications - is reviewed. In the technologies area, high-temperature superconductivity - and especially its use in power-generation facilities - is looked at, as are the topics of energy conversion and thermo-electric power generation. Further, energy storage using compressed-air is discussed. Power distribution and the use of controllable storage in the low-voltage mains is briefly commented on, as is distributed power generation. Information and communications technologies are reviewed, as is work done in the electrical drives area. Work in other areas summarised includes lighting and uninterruptible power-supplies. Co-operation with Swiss institutions and international organisations such as the IEA is reviewed. Implementation work in the pilot and demonstration area is commented on. This includes work in various areas ranging from set-top boxes through to the optimisation of compressed-air systems and electrical drives as well as codes of conduct and agreements for water-dispensers and cooling systems in retail applications and the optimisation of wastewater treatment plant. The report is completed with a list of current research and development projects.

  13. "Kissing bugs": potential disease vectors and cause of anaphylaxis.

    Science.gov (United States)

    Klotz, John H; Dorn, Patricia L; Logan, Joy L; Stevens, Lori; Pinnas, Jacob L; Schmidt, Justin O; Klotz, Stephen A

    2010-06-15

    Physicians in the United States should familiarize themselves with "kissing bugs" endemic to their area of practice and appreciate the medical implications of their bites. Bite victims often seek advice from physicians about allergic reactions as well as the risk of contracting Chagas disease. Physicians are generally knowledgeable about the role of kissing bugs in the transmission of Trypanosoma cruzi in Latin America. However, they may be unaware of (1) severe allergic reactions to kissing bug salivary antigens, (2) the widespread occurrence of T. cruzi amongst vertebrate hosts of kissing bugs, and (3) the incidence of T. cruzi among kissing bugs (T. cruzi may infect >50% of sampled bugs). Despite the potential for Chagas disease transmission, the major concern regarding kissing bugs in the United States is anaphylactic reactions to their bites resulting in frequent emergency department visits, especially in areas of endemicity in the Southwest.

  14. [Worksite physical activity and nutrition programmes: beneficial to our health and wallet?].

    Science.gov (United States)

    van Wier, Marieke F; van Dongen, J M Hanneke; van Tulder, Maurits W

    2013-01-01

    The unhealthy lifestyle of many Dutch employees may negatively influence their health in general as well as their ability to work. Worksite physical activity, nutrition or combination programmes could improve employee health and, as a consequence of this, reduce costs due to illness absenteeism, early retirement, and health care use. In this article, we present current scientific research in terms of health-related effectiveness, cost-effectiveness and the financial return of these programmes. There is moderate evidence that programmes aimed at nutrition, as well as combined nutrition-and-physical activity programmes, do result in modest improvements in weight-related outcomes and the consumption of fruit, vegetables and fat after 6-12 months. There is also moderate evidence that physical fitness programmes improve physical activity and fitness in the short term. The programmes result in financial returns for the employer, but this conclusion is based on non-randomised studies. No firm conclusions can be drawn regarding their cost-effectiveness. Wide implementation of singular worksite physical activity, nutrition or combination programmes is therefore currently discouraged. It would probably be more effective and economical to set up a broad range of interventions and measures. This requires more research, however, as well as more opportunities for offering tailor-made programmes.

  15. What’s in a Kiss? The Effect of Romantic Kissing on Mate Desirability

    Science.gov (United States)

    Wlodarski, Rafael; Dunbar, Robin I. M.

    2015-01-01

    Past research suggests that various courtship rituals, such as romantic kissing, may convey useful mate quality information. Two studies were carried out to examine how purported romantic kissing abilities, as a potential cue to some form of mate information, affect appraisals of potential mating partners. In Experiment 1, 724 participants were presented with vignette descriptions of potential mating partners and were asked to rate partner desirability for various mating-related situations. The primary result of this experiment was that purported kissing ability increased mate desirability in “casual sex” mating situations for women to a greater extent than for men. Experiment 2 repeated the same procedure with another 178 participants, this time including visual information alongside vignette descriptions containing kissing-related information to examine the relative effects of these two modalities. It was found that the presence of a picture alongside a descriptive vignette negated the effect of kissing-related information only when rating potential partners on attractiveness or desirability for further courtship, though not when evaluating partners for casual sex or long-term relationship scenarios. Visual information containing “attractive” photos of potential partners was also found to have a greater effect on men’s ratings of partner desirability than on women’s ratings of partner desirability. The results are discussed in light of romantic kissing’s potential function of conveying important mate quality and desirability information, and its relative role in the presence of additional visual mate cues. PMID:25299759

  16. Divided or kissing nevus of the penis.

    Science.gov (United States)

    Hardin, Carolyn A; Tieu, Kathy D

    2013-10-16

    The divided or kissing nevus is an unusual congenital melanocytic nevus. By definition, these nevi appear on skin that separates during embryological development. These lesions have been reported on the eyelids, fingers, and rarely the penis. We describe an 18 year old uncircumcised male who presented with an asymptomatic darkly pigmented patch on the glans penis. He reported that the lesion had appeared recently and was enlarging. Physical examination revealed a second symmetric lesion on the adjacent foreskin. Punch biopsy of the lesion on the glans penis showed abundant intradermal melanocytes devoid of mitoses and atypia, consistent with an intradermal melanocytic nevus. Based on the benign histologic nature and clinical exam, the lesion was diagnosed as a divided or kissing nevus of the penis. Proposed treatments include excision and grafting as well as Nd:YAG laser therapy. However, these patients may be safely monitored with regular follow-up skin examinations because there is minimal risk of malignant transformation.

  17. Kiss-induced severe anaphylactic reactions

    Directory of Open Access Journals (Sweden)

    Atanasković-Marković Marina

    2010-01-01

    Full Text Available Introduction. Ingestion is the principal route for food allergens to trigger allergic reaction in atopic persons. However, in some highly sensitive patients severe symptoms may develop upon skin contact and by inhalation. The clinical spectrum ranges from mild facial urticaria and angioedema to life-threatening anaphylactic reactions. Outline of Cases. We describe cases of severe anaphylactic reactions by skin contact, induced by kissing in five children with prior history of severe anaphylaxis caused by food ingestion. These cases were found to have the medical history of IgE mediated food allergy, a very high total and specific serum IgE level and very strong family history of allergy. Conclusion. The presence of tiny particles of food on the kisser's lips was sufficient to trigger an anaphylactic reaction in sensitized children with prior history of severe allergic reaction caused by ingestion of food. Allergic reaction provoked with food allergens by skin contact can be a risk factor for generalized reactions. Therefore, extreme care has to be taken in avoiding kissing allergic children after eating foods to which they are highly allergic. Considering that kissing can be a cause of severe danger for the food allergic patient, such persons should inform their partners about the risk factor for causing their food hypersensitivity.

  18. Kiso Supernova Survey (KISS): Survey Strategy

    CERN Document Server

    Morokuma, Tomoki; Tanaka, Masaomi; Mori, Kensho; Matsumoto, Emiko; Kikuchi, Yuki; Shibata, Takumi; Sako, Shigeyuki; Aoki, Tsutomu; Doi, Mamoru; Kobayashi, Naoto; Maehara, Hiroyuki; Matsunaga, Noriyuki; Mito, Hiroyuki; Miyata, Takashi; Nakada, Yoshikazu; Soyano, Takao; Tarusawa, Ken'ichi; Miyazaki, Satoshi; Nakata, Fumiaki; Okada, Norio; Sarugaku, Yuki; Richmond, Michael W; Akitaya, Hiroshi; Aldering, Greg; Arimatsu, Ko; Contreras, Carlos; Horiuchi, Takashi; Hsiao, Eric Y; Itoh, Ryosuke; Iwata, Ikuru; Koji,; Kawabata, S; Kawai, Nobuyuki; Kitagawa, Yutaro; Kokubo, Mitsuru; Kuroda, Daisuke; Paolo,; Mazzali,; Misawa, Toru; Moritani, Yuki; Morrell, Nidia; Okamoto, Rina; Pavlyuk, Nikolay; Phillips, Mark M; Pian, Elena; Sahu, Devendra; Saito, Yoshihiko; Sano, Kei; Stritzinger, Maximilian D; Tachibana, Yutaro; Taddia, Francesco; Takaki, Katsutoshi; Tateuchi, Ken; Tomita, Akihiko; Tsvetkov, Dmitry; Ui, Takahiro; Ukita, Nobuharu; Urata, Yuji; Walker, Emma S; Yoshii, Taketoshi

    2014-01-01

    The Kiso Supernova Survey (KISS) is a high-cadence optical wide-field supernova (SN) survey. The primary goal of the survey is to catch the very early light of a SN, during the shock breakout phase. Detection of SN shock breakouts combined with multi-band photometry obtained with other facilities would provide detailed physical information on the progenitor stars of SNe. The survey is performed using a 2.2x2.2 deg field-of-view instrument on the 1.05-m Kiso Schmidt telescope, the Kiso Wide Field Camera (KWFC). We take a three-minute exposure in g-band once every hour in our survey, reaching magnitude g~20-21. About 100 nights of telescope time per year have been spent on the survey since April 2012. The number of the shock breakout detections is estimated to be of order of 1 during our 3-year project. This paper summarizes the KISS project including the KWFC observing setup, the survey strategy, the data reduction system, and CBET-reported SNe discovered so far by KISS.

  19. Kiso Supernova Survey (KISS): Survey strategy

    Science.gov (United States)

    Morokuma, Tomoki; Tominaga, Nozomu; Tanaka, Masaomi; Mori, Kensho; Matsumoto, Emiko; Kikuchi, Yuki; Shibata, Takumi; Sako, Shigeyuki; Aoki, Tsutomu; Doi, Mamoru; Kobayashi, Naoto; Maehara, Hiroyuki; Matsunaga, Noriyuki; Mito, Hiroyuki; Miyata, Takashi; Nakada, Yoshikazu; Soyano, Takao; Tarusawa, Ken'ichi; Miyazaki, Satoshi; Nakata, Fumiaki; Okada, Norio; Sarugaku, Yuki; Richmond, Michael W.; Akitaya, Hiroshi; Aldering, Greg; Arimatsu, Ko; Contreras, Carlos; Horiuchi, Takashi; Hsiao, Eric Y.; Itoh, Ryosuke; Iwata, Ikuru; Kawabata, Koji S.; Kawai, Nobuyuki; Kitagawa, Yutaro; Kokubo, Mitsuru; Kuroda, Daisuke; Mazzali, Paolo; Misawa, Toru; Moritani, Yuki; Morrell, Nidia; Okamoto, Rina; Pavlyuk, Nikolay; Phillips, Mark M.; Pian, Elena; Sahu, Devendra; Saito, Yoshihiko; Sano, Kei; Stritzinger, Maximilian D.; Tachibana, Yutaro; Taddia, Francesco; Takaki, Katsutoshi; Tateuchi, Ken; Tomita, Akihiko; Tsvetkov, Dmitry; Ui, Takahiro; Ukita, Nobuharu; Urata, Yuji; Walker, Emma S.; Yoshii, Taketoshi

    2014-12-01

    The Kiso Supernova Survey (KISS) is a high-cadence optical wide-field supernova (SN) survey. The primary goal of the survey is to catch the very early light of a SN, during the shock breakout phase. Detection of SN shock breakouts combined with multi-band photometry obtained with other facilities would provide detailed physical information on the progenitor stars of SNe. The survey is performed using a 2.2° × 2.2° field-of-view instrument on the 1.05-m Kiso Schmidt telescope, the Kiso Wide Field Camera (KWFC). We take a 3-min exposure in g-band once every hour in our survey, reaching magnitude g ˜ 20-21. About 100 nights of telescope time per year have been spent on the survey since 2012 April. The number of the shock breakout detections is estimated to be of the order of 1 during our three-year project. This paper summarizes the KISS project including the KWFC observing setup, the survey strategy, the data reduction system, and CBET-reported SNe discovered so far by KISS.

  20. Differential regulation of kiss1 expression by melatonin and gonadal hormones in male and female Syrian hamsters

    DEFF Research Database (Denmark)

    Ansel, L; Bolborea, M; Bentsen, A H

    2010-01-01

    In seasonal breeders, reproduction is synchronized to seasons by day length via the pineal hormone melatonin. Recently, we have demonstrated that Kiss1, a key activator of the reproductive function, is down-regulated in sexually inactive hamsters maintained in inhibitory short days (SDs). In rode......In seasonal breeders, reproduction is synchronized to seasons by day length via the pineal hormone melatonin. Recently, we have demonstrated that Kiss1, a key activator of the reproductive function, is down-regulated in sexually inactive hamsters maintained in inhibitory short days (SDs......). In rodents, Kiss1 is expressed in the anteroventral periventricular nucleus (AVPV) and in the arcuate nucleus (ARC). Because both the duration of the nocturnal peak of melatonin and circulating sex steroid levels vary with photoperiod, the aim of this study was to determine whether melatonin and sex steroids...... as compared to LD animals, 2) sex steroid treatment in SD-adapted male and female hamsters increased the number of Kiss1 neurons in the AVPV but decreased it in the ARC, 3) melatonin administration to LD-adapted hamsters decreased Kiss1 mRNA level in both the AVPV and the ARC in intact animals, whereas...

  1. The Effect of Sanctions and Active Labour Market Programmes on the Exit Rate From Unemployment

    DEFF Research Database (Denmark)

    Ahmad, Nisar; Svarer, Michael

    ). Hence, modeling only one of them as treatment might over or underestimate the true effect. Therefore, by using a multivariate mixed proportional hazard model (MMPH), we model the hazard rate out of unemployment along with the sanction rate and hazard rate into active labour market programmes. We......This paper simultaneously investigates the effectiveness of benefit sanctions and active labour market programmes on the exit rate from unemployment using Danish data. In the data about one third of the individuals who are sanctioned also participate in some active labour market programmes (ALMPs...... optimally select the number of supports point for the distribution of unobserved heterogeneity. Results show that pre-specifying two support points underestimates the effect of sanctions and active labour market programmes. Failing to control for selectivity for sanctions not only underestimates...

  2. The Effect of Sanctions and Active Labour Market Programmes on the Exit Rate From Unemployment

    DEFF Research Database (Denmark)

    Ahmad, Nisar; Svarer, Michael

    2009-01-01

    ). Hence, modeling only one of them as treatment might over or underestimate the true effect. Therefore, by using a multivariate mixed proportional hazard model (MMPH), we model the hazard rate out of unemployment along with the sanction rate and hazard rate into active labour market programmes. We......This paper simultaneously investigates the effectiveness of benefit sanctions and active labour market programmes on the exit rate from unemployment using Danish data. In the data about one third of the individuals who are sanctioned also participate in some active labour market programmes (ALMPs...... optimally select the number of supports point for the distribution of unobserved heterogeneity. Results show that pre-specifying two support points underestimates the effect of sanctions and active labour market programmes. Failing to control for selectivity for sanctions not only underestimates...

  3. The Effect of Sanctions and Active Labour Market Programmes on the Exit Rate From Unemployment

    DEFF Research Database (Denmark)

    Ahmad, Nisar; Svarer, Michael

    2009-01-01

    ). Hence, modeling only one of them as treatment might over or underestimate the true effect. Therefore, by using a multivariate mixed proportional hazard model (MMPH), we model the hazard rate out of unemployment along with the sanction rate and hazard rate into active labour market programmes. We......This paper simultaneously investigates the effectiveness of benefit sanctions and active labour market programmes on the exit rate from unemployment using Danish data. In the data about one third of the individuals who are sanctioned also participate in some active labour market programmes (ALMPs...... optimally select the number of supports point for the distribution of unobserved heterogeneity. Results show that pre-specifying two support points underestimates the effect of sanctions and active labour market programmes. Failing to control for selectivity for sanctions not only underestimates...

  4. Piloting a physical activity centred education programme for adults with a brain injury.

    Science.gov (United States)

    Driver, Simon; Irwin, Kelley; Woolsey, Anne; Warren, Ann Marie

    2013-01-01

    To pilot test a physical activity centred education (PACE) programme for adults with a brain injury. Purposive sampling was utilized from a convenience sample of nine adults with a brain injury (five female; four male). The sample completed an 8-week health promotion programme focused on improving physical activity behaviours. Participants completed measures of self-efficacy, stage of change and rehabilitation outcomes pre- and post-programme. Descriptive analysis, effect sizes (ES) and percentage change in variables were assessed. Results indicated improved rehabilitation outcomes as participants decreased from moderate to mild limitation (ES = 1.67). Furthermore, participants reported increased self-efficacy (ES = 0.41) and intention to be active. Specialists are challenged to find modes of rehabilitation that improve the health of individuals with a brain injury. Pilot results from the PACE programme indicate that education about physical activity may play an important role in the rehabilitation process and lead to improved health outcomes.

  5. Combustion chemistry - activities in the CHEK research programme

    Energy Technology Data Exchange (ETDEWEB)

    Dam-Johansen, K.; Johnsson, J.E.; Glarborg, P.; Frandsen, F.; Jensen, A.; Oestberg, M. [Technical Univ. of Denmark, Lyngby (Denmark). Dept. of Chemical Engineering

    1997-10-01

    The combustion chemistry in the oxidation of fossil fuels and biofuels determines together with mixing and heat transfer the required size of a furnace, the emission of gaseous pollutants, and the formation of ash and deposits on surfaces. This presentation describes technologies for solid fuels combustion and gives a summary of the fuels, the pollutant chemistry and the inorganic chemistry in combustion processes. Emphasis is put on the work carried out in the CHEC (Combustion and Harmful Emission Control) Research Programme. (orig.)

  6. Active Labour Market Programme Participation for Unemployment Insurance Recipients: A Systematic Review

    DEFF Research Database (Denmark)

    Filges, Trine; Smedslund, Geir; Knudsen, Anne-Sofie Due

    2015-01-01

    BACKGROUND During the 1990s, many countries introduced Active Labour Market Programmes (ALMPs) in an effort to reduce unemployment. The introduction of ALMPs is often motivated by the need to upgrade the skills of especially those suffering long-term unemployment to improve their productivity and...... assistance and education) are part-time and have a short duration (e.g. few days/weeks). It is possible to classify these programmes into a set of four core categories: A: (labour market) training, B: Private sector programmes, C: direct employment programmes in the public sector and D: Job search assistance....... The categories we use broadly correspond to classifications that have been suggested and used by the OECD and Eurostat (OECD, 2004 and Eurostat, 2005), even though there are differences between OECD and Eurostat in how they define and categorise these programmes. OBJECTIVES The objective of this systematic...

  7. T.S.K.H.: Tickle Snug Kiss Hug; Exercises and Tricks for Parent-Child Fun.

    Science.gov (United States)

    May, Betty

    This book presents a collection of physical activities for parents and children. Although all of the activities provide some exercise and physical conditioning, the book emphasizes having fun. The book encourages the physical closeness of tickling, snuggling, kissing and hugging (TSKH) as an important part of each activity. Each activity…

  8. Malt1 and cIAP2-Malt1 as effectors of NF-kappaB activation: kissing cousins or distant relatives?

    Science.gov (United States)

    Kingeter, Lara M; Schaefer, Brian C

    2010-01-01

    Malt1 is a multi-domain cytosolic signaling molecule that was originally identified as the target of recurrent translocations in a large fraction of MALT lymphomas. The product of this translocation is a chimeric protein in which the N-terminus is contributed by the apoptosis inhibitor, cIAP2, and the C-terminus is contributed by Malt1. Early studies suggested that Malt1 is an essential intermediate in antigen receptor activation of NF-kappaB, and that the juxtaposition of the cIAP2 N-terminus and the Malt1 C-terminus results in deregulation of Malt1 NF-kappaB stimulatory activity. Initial experimental data further suggested that the molecular mechanisms of Malt1- and cIAP-Malt1-mediated NF-kappaB activation were quite similar. However, a number of more recent studies of both Malt1 and cIAP2-Malt1 now reveal that these proteins influence NF-kappaB activation by multiple distinct mechanisms, several of which are non-overlapping. Currently available data suggest a revised model in which cIAP2-Malt1 induces NF-kappaB activation via a mechanism that depends equally on domains contributed by cIAP2 and Malt1, which confer spontaneous oligomerization activity, polyubiquitin binding, proteolytic activity, and association with and activation of TRAF2 and TRAF6 at several independent binding sites. By contrast, emerging data suggest that the wild-type Malt1 protein uniquely contributes to NF-kappaB activation primarily through the control of two proteolytic cleavage mechanisms. Firstly, Malt1 directly cleaves and inactivates A20, a negative regulator of the antigen receptor-to-NF-kappaB pathway. Secondly, Malt1 interacts with caspase-8, inducing caspase-8 cleavage of c-FLIP(L), initiating a pathway that contributes to activation of the I kappaB kinase (IKK) complex. Furthermore, data suggest that Malt1 plays a more limited and focused role in antigen receptor activation of NF-kappaB, serving to augment weak antigen signals and stimulate a defined subset of NF

  9. High accuracy semidefinite programming bounds for kissing numbers

    NARCIS (Netherlands)

    Mittelmann, H.D.; Vallentin, F.

    2009-01-01

    The kissing number in n-dimensional Euclidean space is the maximal number of non-overlapping unit spheres which simultaneously can touch a central unit sphere. Bachoc and Vallentin developed a method to find upper bounds for the kissing number based on semidefinite programming. This paper is a repor

  10. Long term results of kissing stents in the aortic bifurcation

    NARCIS (Netherlands)

    Hinnen, J.W.; Konickx, M.A.; Meerwaldt, Robbert; Kolkert, J.L.P.; van der Palen, Jacobus Adrianus Maria; Huisman, A.B.

    2015-01-01

    BACKGROUND: To evaluate the long-term outcome after aortoiliac kissing stent placement and to analyze variables, which potentially influence the outcome of endovascular reconstruction of the aortic bifurcation. METHODS: All patients treated with aortoiliac kissing stents at our institution between

  11. High accuracy semidefinite programming bounds for kissing numbers

    NARCIS (Netherlands)

    H.D. Mittelmann; F. Vallentin (Frank)

    2010-01-01

    htmlabstractThe kissing number in n-dimensional Euclidean space is the maximal number of non-overlapping unit spheres which simultaneously can touch a central unit sphere. Bachoc and Vallentin developed a method to find upper bounds for the kissing number based on semidefinite programming. This

  12. High accuracy semidefinite programming bounds for kissing numbers

    NARCIS (Netherlands)

    H.D. Mittelmann; F. Vallentin (Frank)

    2009-01-01

    htmlabstractThe kissing number in n-dimensional Euclidean space is the maximal number of non-overlapping unit spheres which simultaneously can touch a central unit sphere. Bachoc and Vallentin developed a method to find upper bounds for the kissing number based on semidefinite programming. This

  13. From activity to learning: using cultural historical activity theory to model school library programmes and practices

    Directory of Open Access Journals (Sweden)

    E.M. Meyers

    2007-01-01

    Full Text Available Introduction. changes in educational policy and practice demand that we examine school library programmes from a new perspective. As a model that takes a developmental view of minds in context, Cultural Historical Activity Theory is particularly well suited to the study of school libraries and the learning that occurs therein. This paper focuses on the activity theoretic concepts of 'contradictions' and 'expansive learning' as they relate to the development of best practices. Method. Developmental Work Research was applied as a guiding methodology in an intervention study of six high school libraries in Washington State, USA. Library activity is illustrated from multiple perspectives using a triangulated, qualitative approach. Analysis. contradictions and tensions in the general school library activity system are identified, and an intervention was designed to facilitiate the development of expansive instruments. A case example illustrates a second level of analysis and specific points of intervention. Results. analysis reveals that the tensions and contradictions provide opportunities for expansive learning on the part of the teacher-Librarians. The research team can use practitioners' zones of proximal development to guide the alignment of library programme goals and practices. Conclusion. : some limitations and future promise of the framework and its application are discussed. The proposed activity theory toolkit suggest a new way of exploring the practices of teacher-librarians, incorporating research evidence, professional expertise, and reflective decision making.

  14. Expression of KISS1 and KISS1R (GPR54) may be used as favorable prognostic markers for patients with non-small cell lung cancer.

    Science.gov (United States)

    Sun, Yan-Bin; Xu, Shun

    2013-08-01

    Lung cancer is the most commonly diagnosed cancer worldwide. Loss of KISS1 expression has been associated with progression and poor prognosis of various cancers, however, the precise role of KISS1 expression in non-small cell lung cancer (NSCLC) is not well defined. KISS1 receptor (KISS1R, also named GPR54) coupled to KISS1, has been shown to play a pivotal role in suppressing cancer metastasis. In this study, 56 NSCLC specimens were divided into stage IIIB (locally advanced) and stage IV (metastatic). The mRNA and protein levels of KISS1 and KISS1R in cancer tissues were found to be lower compared to that in normal tissues using RT-PCR and western blot analysis, respectively. In addition, the expression of both KISS1 and KISS1R in stage IV NSCLC was lower compared to that in stage IIIB stage NSCLC. The cumulative survival rate of the patients with KISS1 or KISS1R expression was significantly higher compared to that without expression. KISS1 or KISS1R expression in NSCLC can be used to indicate favorable prognosis for disease outcome. Metastin, the product of the KISS1 gene, was lower in the serum of patients with stage IV NSCLC compared to that in stage IIIB NSCLC.

  15. Prognostic role of KiSS-1 and possibility of therapeutic modality of metastin, the final peptide of the KiSS-1 gene, in urothelial carcinoma.

    Science.gov (United States)

    Takeda, Toshikazu; Kikuchi, Eiji; Mikami, Shuji; Suzuki, Eriko; Matsumoto, Kazuhiro; Miyajima, Akira; Okada, Yasunori; Oya, Mototsugu

    2012-04-01

    The KiSS-1 gene has been reported to be a metastasis suppressor gene in human melanoma. The gene product was isolated from human placenta as the ligand of GPR54, a G protein-coupled receptor, and the C-terminally amidated peptide of 54 amino acids is called metastin. The binding of metastin to GPR54 has been shown to inhibit tumor metastasis in some tumor cells; however, its function remains unclear in urothelial carcinoma. We first evaluated KiSS-1 expression and GPR54 expression in 151 patients with upper urinary tract urothelial carcinoma to determine their prognostic significance. Next, we examined the role of metastin in the invasiveness and lung metastasis of MBT-2 variant (MBT-2V), which is a highly metastatic murine bladder cancer cell. Multivariate analysis revealed that KiSS-1 expression was an independent predictor of metastasis and overall survival. However, GPR54 expression was not selected. Hematogeneous metastasis had a significantly lower level of KiSS-1 expression compared with lymph node metastasis. Metastin treatment significantly reduced the invasiveness of MBT-2V cells and inhibited the DNA-binding activity of NF-κB by blocking its nuclear translocation, leading to a reduction in the expression and activity of matrix metalloproteinase-9. Metastin treatment dramatically prevented the occurrence of lung metastatic nodules (6.3 ± 2.3, n = 15) compared with controls (30.4 ± 5.1, n = 15; P metastin may be an effective inhibitor of metastasis in urothelial carcinoma through its blockade of NF-κB function.

  16. Combustion chemistry. Activities in the CHEC research programme

    Energy Technology Data Exchange (ETDEWEB)

    Dam-Johansen, K.; Johnsson, J.E.; Glarborg, P.; Frandsen, F.; Jensen, A.; Oestberg, M. [Technical Univ. of Denmark, Dept. of Chemical Engineering, Lyngby (Denmark)

    1996-12-01

    The combustion chemistry in the oxidation of fossil fuels and biofuels determines together with mixing and heat transfer the required size of a furnace, the emission of gaseous pollutants, and the formation of ash and deposits on surfaces. This paper describes technologies for solid fuels combustion and gives a summary of the fuels, the pollutant chemistry and the inorganic chemistry in combustion processes. Emphasis is put on the work carried out in the CHEC (Combustion and Harmful Emission Control Research Programme). (au) 173 refs.

  17. Adult Workers in Theory or Practice? : Lone Mothers’ Participation in Active Labour Market Programmes in Germany

    Directory of Open Access Journals (Sweden)

    Cordula Zabel

    2015-03-01

    Full Text Available This paper examines lone mothers’ participation in active labour market programmes in Germany. Since the 2005 Hartz IV employment and welfare policy reforms, expectations that non-em- ployed parents receiving means-tested benefits should be ready for employment or labour market programme participation have grown stronger. However, discretion for programme assignments is left to individual caseworkers. As a consequence, it is not clear to what extent the formal policy orientation towards an adult worker model of the family is reflected in practical policy implemen- tations. Thus, lone mothers’ participation in active labour market programmes is studied empiri- cally here on the basis of large-scale administrative data, using event-history analysis. Findings are that lone mothers are treated as adult workers with respect to workfare and training pro- grammes even when their children are still quite young. As soon as their youngest child is 3 - 5 years old, lone mothers’ transition rates into these programmes are as high as for childless single women. In the case of programmes that provide more direct pathways into regular employment, like job subsidies and in-firm training programmes, however, participation rates for lone mothers of young children are substantially lower than for childless single women.

  18. Long-term effect of a school-based physical activity program (KISS on fitness and adiposity in children: a cluster-randomized controlled trial.

    Directory of Open Access Journals (Sweden)

    Ursina Meyer

    Full Text Available School-based intervention studies promoting a healthy lifestyle have shown favorable immediate health effects. However, there is a striking paucity on long-term follow-ups. The aim of this study was therefore to assess the 3 yr-follow-up of a cluster-randomized controlled school-based physical activity program over nine month with beneficial immediate effects on body fat, aerobic fitness and physical activity.Initially, 28 classes from 15 elementary schools in Switzerland were grouped into an intervention (16 classes from 9 schools, n = 297 children and a control arm (12 classes from 6 schools, n = 205 children after stratification for grade (1st and 5th graders. Three years after the end of the multi-component physical activity program of nine months including daily physical education (i.e. two additional lessons per week on top of three regular lessons, short physical activity breaks during academic lessons, and daily physical activity homework, 289 (58% participated in the follow-up. Primary outcome measures included body fat (sum of four skinfolds, aerobic fitness (shuttle run test, physical activity (accelerometry, and quality of life (questionnaires. After adjustment for grade, gender, baseline value and clustering within classes, children in the intervention arm compared with controls had a significantly higher average level of aerobic fitness at follow-up (0.373 z-score units [95%-CI: 0.157 to 0.59, p = 0.001] corresponding to a shift from the 50th to the 65th percentile between baseline and follow-up, while the immediate beneficial effects on the other primary outcomes were not sustained.Apart from aerobic fitness, beneficial effects seen after one year were not maintained when the intervention was stopped. A continuous intervention seems necessary to maintain overall beneficial health effects as reached at the end of the intervention.ControlledTrials.com ISRCTN15360785.

  19. Long-term effect of a school-based physical activity program (KISS) on fitness and adiposity in children: a cluster-randomized controlled trial.

    Science.gov (United States)

    Meyer, Ursina; Schindler, Christian; Zahner, Lukas; Ernst, Dominique; Hebestreit, Helge; van Mechelen, Willem; Brunner-La Rocca, Hans-Peter; Probst-Hensch, Nicole; Puder, Jardena J; Kriemler, Susi

    2014-01-01

    School-based intervention studies promoting a healthy lifestyle have shown favorable immediate health effects. However, there is a striking paucity on long-term follow-ups. The aim of this study was therefore to assess the 3 yr-follow-up of a cluster-randomized controlled school-based physical activity program over nine month with beneficial immediate effects on body fat, aerobic fitness and physical activity. Initially, 28 classes from 15 elementary schools in Switzerland were grouped into an intervention (16 classes from 9 schools, n = 297 children) and a control arm (12 classes from 6 schools, n = 205 children) after stratification for grade (1st and 5th graders). Three years after the end of the multi-component physical activity program of nine months including daily physical education (i.e. two additional lessons per week on top of three regular lessons), short physical activity breaks during academic lessons, and daily physical activity homework, 289 (58%) participated in the follow-up. Primary outcome measures included body fat (sum of four skinfolds), aerobic fitness (shuttle run test), physical activity (accelerometry), and quality of life (questionnaires). After adjustment for grade, gender, baseline value and clustering within classes, children in the intervention arm compared with controls had a significantly higher average level of aerobic fitness at follow-up (0.373 z-score units [95%-CI: 0.157 to 0.59, p = 0.001] corresponding to a shift from the 50th to the 65th percentile between baseline and follow-up), while the immediate beneficial effects on the other primary outcomes were not sustained. Apart from aerobic fitness, beneficial effects seen after one year were not maintained when the intervention was stopped. A continuous intervention seems necessary to maintain overall beneficial health effects as reached at the end of the intervention. ControlledTrials.com ISRCTN15360785.

  20. Mecanismos celulares y moleculares de acción del sistema kiss1/kiss1r en la hipófisis

    OpenAIRE

    Gutiérrez Pascual, María Ester

    2011-01-01

    Las kisspeptinas (kps) son una familia de péptidos derivados de un precursor común codificado por el gen kiss1, caracterizado inicialmente por su capacidad para inhibir la metástasis tumoral a través de su receptor kiss1r. Más recientemente, numerosos estudios han demostrado que el sistema kiss1/kiss1r juega un papel esencial en el control neuroendocrino del eje reproductor, donde actúa estimulando la liberación de GnRH a nivel hipotalámico. Sin embargo, la expresión de kiss1 y kiss1r en teji...

  1. Malt1 and cIAP2-Malt1 as effectors of NF-κB activation: Kissing cousins or distant relatives?

    OpenAIRE

    Kingeter, Lara M.; Schaefer, Brian C.

    2009-01-01

    Malt1 is a multi-domain cytosolic signaling molecule that was originally identified as the target of recurrent translocations in a large fraction of MALT lymphomas. The product of this translocation is a chimeric protein in which the N-terminus is contributed by the apoptosis inhibitor, cIAP2, and the C-terminus is contributed by Malt1. Early studies suggested that Malt1 is an essential intermediate in antigen receptor activation of NF-κB, and that the juxtaposition of the cIAP2 N-terminus an...

  2. KiSS-1 expression in human breast cancer.

    Science.gov (United States)

    Martin, Tracey A; Watkins, Gareth; Jiang, Wen G

    2005-01-01

    The KiSS-1 gene encodes a 145 amino acid residue peptide that is further processed to a final peptide, metastin, a ligand to a G-coupled orphan receptor (OT7T175/AXOR12). KiSS-1 has been identified as a putative human metastasis suppressor gene in melanomas and in breast cancer cell lines. This study aimed to determine the expression and distribution of KiSS-1 and its receptor in human breast cancer tissues and to identify a possible link between expression levels and patient prognosis. Frozen sections from breast cancer primary tumours (matched tumour 124 and background 33) were immuno-stained with KiSS-1 antibody. RNA was reverse transcribed and analyzed by Q-PCR (standardized using beta-actin, and normalized with cytokeratin-19 levels). Levels of expression of KiSS-1 were higher in tumour compared to background tissues (3,124+/-1,262 vs 2,397+/-1,181) and significantly increased in node positive tumours compared to node negative (3,637+/-1,719 vs 2,653+/-1,994, P = 0.02). KiSS-1 expression was also increased with increasing grade and TNM status. There were no such trends with the KiSS-1 receptor. Expression of KiSS-1 was higher in patients who had died from breast cancer than those who had remained healthy (4,631+/-3,024 vs 2,280+/-1,403) whereas expression of the receptor was reduced (480+/-162 vs 195+/-134). Immunohistochemical staining showed increased expression of KiSS-1 in tumour sections. Insertion of the KiSS-1 gene into the human breast cancer cell line MDA-MB-231, resulted in cells that were significantly more motile and invasive in behaviour, with reduced adhesion to matrix, using respective assays. In conclusion, KiSS-1 expression is increased in human breast cancer, particularly in patients with aggressive tumours and with mortality. Over-expression of KiSS-1 in breast cancer cells result in more aggressive phenotype. Together, it suggests that KiSS-1 plays a role beyond the initial metastasis repressor in this cancer type.

  3. Objects prompt authentic scientific activities among learners in a museum programme

    Science.gov (United States)

    Achiam, Marianne; Simony, Leonora; Kramer Lindow, Bent Erik

    2016-04-01

    Although the scientific disciplines conduct practical work in different ways, all consider practical work as the essential way of connecting objects and phenomena with ideas and the abstract. Accordingly, practical work is regarded as central to science education as well. We investigate a practical, object-based palaeontology programme at a natural history museum to identify how palaeontological objects prompt scientific activity among upper secondary school students. We first construct a theoretical framework based on an analysis of the programme's palaeontological content. From this, we build our reference model, which considers the specimens used in the programme, possible palaeontological interpretations of these specimens, and the conditions inherent in the programme. We use the reference model to analyse the activities of programme participants, and illustrate how these activities are palaeontologically authentic. Finally, we discuss our findings, examining the mechanism by which the specimens prompt scientific activities. We also discuss our discipline-based approach, and how it allows us to positively identify participants' activities as authentic. We conclude by discussing the implications of our findings.

  4. A FRET Biosensor for ROCK Based on a Consensus Substrate Sequence Identified by KISS Technology.

    Science.gov (United States)

    Li, Chunjie; Imanishi, Ayako; Komatsu, Naoki; Terai, Kenta; Amano, Mutsuki; Kaibuchi, Kozo; Matsuda, Michiyuki

    2017-01-11

    Genetically-encoded biosensors based on Förster/fluorescence resonance energy transfer (FRET) are versatile tools for studying the spatio-temporal regulation of signaling molecules within not only the cells but also tissues. Perhaps the hardest task in the development of a FRET biosensor for protein kinases is to identify the kinase-specific substrate peptide to be used in the FRET biosensor. To solve this problem, we took advantage of kinase-interacting substrate screening (KISS) technology, which deduces a consensus substrate sequence for the protein kinase of interest. Here, we show that a consensus substrate sequence for ROCK identified by KISS yielded a FRET biosensor for ROCK, named Eevee-ROCK, with high sensitivity and specificity. By treating HeLa cells with inhibitors or siRNAs against ROCK, we show that a substantial part of the basal FRET signal of Eevee-ROCK was derived from the activities of ROCK1 and ROCK2. Eevee-ROCK readily detected ROCK activation by epidermal growth factor, lysophosphatidic acid, and serum. When cells stably-expressing Eevee-ROCK were time-lapse imaged for three days, ROCK activity was found to increase after the completion of cytokinesis, concomitant with the spreading of cells. Eevee-ROCK also revealed a gradual increase in ROCK activity during apoptosis. Thus, Eevee-ROCK, which was developed from a substrate sequence predicted by the KISS technology, will pave the way to a better understanding of the function of ROCK in a physiological context.

  5. Till Surgery do us Part: Unexpected Bilateral Kissing Molars.

    Science.gov (United States)

    Anish, Narayanankutty; Vivek, Velayudhannair; Thomas, Sunila; Daniel, Vineet Alex; Thomas, Jincy; Ranimol, Prasanna

    2015-01-28

    The occurrence impacted teeth, single or multiple is very common. But, phenomenon of kissing molars is an extremely rare phenomenon. Mandibular third molars are the most common impacted teeth. Mandibular first or second molars does not share the same frequency of occurrence. But, there are rare cases in which the occlusal surfaces of impacted molars are united by the same follicular space and the roots point in the opposite direction, and are termed as kissing molars. Sometimes, these teeth will be associated with pathologies. This article reports a rare case of mandibular bilateral kissing molars.

  6. Till surgery do us part: unexpected bilateral kissing molars

    Directory of Open Access Journals (Sweden)

    Narayanankutty Anish

    2015-02-01

    Full Text Available The occurrence impacted teeth, single or multiple is very common. But, phenomenon of kissing molars is an extremely rare phenomenon. Mandibular third molars are the most common impacted teeth. Mandibular first or second molars does not share the same frequency of occurrence. But, there are rare cases in which the occlusal surfaces of impacted molars are united by the same follicular space and the roots point in the opposite direction, and are termed as kissing molars. Sometimes, these teeth will be associated with pathologies. This article reports a rare case of mandibular bilateral kissing molars.

  7. [Physical activity programmes to reduce overweight and obesity in children and adolescents; a systematic review].

    Science.gov (United States)

    Aguilar Cordero, M J; Ortegón Piñero, A; Mur Vilar, N; Sánchez García, J C; García Verazaluce, J J; García García, I; Sánchez López, A M

    2014-10-01

    Obesity treatment has been the subject of much controversy; various authors have recommended the application of a comprehensive treatment programme, and in the light of this previous research, we consider the question of what is the most effective programme of physical activity to reduce overweight and obesity in children and adolescents. To analyse major studies on the effectiveness of physical activity in reducing overweight and obesity in children and adolescents. Systematic review of the results of physical activity programmes, published in scientific articles, to reduce overweight and obesity. Using an automated database search in PubMed and Google Scholar, conducted from October 2013 to March 2014, we identified 85 valid items. In selecting the items, the criteria applied included the usefulness and relevance of the subject matter and the credibility or experience of the research study authors. The internal and external validity of each of the articles reviewed was taken into account. This review confirmed the effectiveness of physical activity in reducing overweight and obesity in children and adolescents. The most effective programmes were those combining aerobic and anaerobic exercises. It is generally accepted that at least 180 minutes per week should be dedicated to exercise, in the form of three 60-minute sessions of moderate intensity. Such programmes could be sufficient for persons with overweight or obesity. Researchers in this field agree that when a diet based on an appropriate distribution of meals is combined with regular physical activity, they reinforce each other, and thus optimum results are obtained. Weight reduction programmes that take account of family involvement are more effective than nutrition education itself or other routine interventions that fail to consider family involvement. The role of pa rents and of the persons around the child or adolescent is essential to reinforce positive behaviour toward lifestyle change. Copyright AULA

  8. Family matters: Directionality of turning bias while kissing is modulated by context.

    Science.gov (United States)

    Sedgewick, Jennifer R; Elias, Lorin J

    2016-01-28

    When leaning forward to kiss to a romantic partner, individuals tend to direct their kiss to the right more often than the left. Studies have consistently demonstrated this kissing asymmetry, although other factors known to influence lateral biases, such as sex or situational context, had yet to be explored. The primary purpose of our study was to investigate if turning direction was consistent between a romantic (parent-parent) and parental (parent-child) kissing context, and secondly, to examine if sex differences influenced turning bias between parent-child kissing partners. An archival analysis coded the direction of turning bias for 161 images of romantic kissing (mothers kissing fathers) and 529 images of parental kissing (mothers or fathers kissing sons or daughters). The results indicated that the direction of turning bias differed between kissing contexts. As expected, a right-turn bias was observed for romantic kissing; however, a left-turn bias was exhibited for parental kissing. There was no significant difference of turning bias between any parent-child kissing partners. Interpretations for the left-turn bias discuss parental kissing as a learned lateral behaviour.

  9. Off-line test of the KISS gas cell

    Energy Technology Data Exchange (ETDEWEB)

    Hirayama, Yoshikazu, E-mail: yoshikazu.hirayama@kek.jp [Institute of Particle and Nuclear Studies (IPNS), High Energy Accelerator Research Organization (KEK), Ibaraki 305-0801 (Japan); Watanabe, Yutaka; Imai, Nobuaki; Ishiyama, Hironobu; Jeong, Sun-Chan; Miyatake, Hiroari; Oyaizu, Michihiro [Institute of Particle and Nuclear Studies (IPNS), High Energy Accelerator Research Organization (KEK), Ibaraki 305-0801 (Japan); Kim, Yung Hee [Seoul National University, Seoul 151 742 (Korea, Republic of); Mukai, Momo [Tsukuba University, Ibaraki 305 0006 (Japan); Matsuo, Yukari; Sonoda, Tetsu; Wada, Michiharu [Nishina Center for Accelerator-Based Science, RIKEN, Wako, Saitama 351 0198 (Japan); Huyse, Mark; Kudryavtsev, Yuri; Van Duppen, Piet [Instituut voor Kern-en Stralingsfysica, KU Leuven, B-3001 Leuven (Belgium)

    2013-12-15

    Highlights: • Construction of the KEK Isotope Separation System (KISS) at RIKEN. • Ionization scheme of an iron. • Measurement of transport time profile in a gas cell. -- Abstract: The KEK Isotope Separation System (KISS) has been constructed at RIKEN to study the β-decay properties of neutron-rich isotopes with neutron numbers around N = 126 for application to astrophysics. A key component of KISS is a gas cell filled with argon gas at a pressure of 50 kPa to stop and collect the unstable nuclei, where the isotopes of interest will be selectively ionized using laser resonance ionization. We have performed off-line tests to study the basic properties of the gas cell and of KISS using nickel and iron filaments placed in the gas cell.

  10. Kissing anterior communicating artery aneurysms: Diagnostic dilemma and management issues

    Directory of Open Access Journals (Sweden)

    S S Baldawa

    2011-01-01

    Full Text Available Kissing aneurysms are unusual and relatively rare types of multiple intracranial arterial aneurysms. When located on the anterior communicating artery (ACoA, kissing aneurysms pose considerable diagnostic difficulty on preoperative conventional angiogram. Special angiographic views or 3D rotational angiogram are needed to make the correct diagnosis and to avoid interpreting them as multilobed or bilobed saccular aneurysms on preoperative conventional angiogram. Treatment of these aneurysms, either by clipping or coiling, needs to be individualized. Unique problems which need to be addressed during surgical clipping are high risk of rupture due to dense adhesions between the kissing aneurysms, requirement of at least two clips in a narrow working area, the aneurysm that needs to be clipped first and interference of the first clip with application of subsequent clips. The authors present a case of a 63-year-old male who had kissing ACoA aneurysms managed successfully by clipping.

  11. Study of Kissing Molars in Turkish Population Sample

    African Journals Online (AJOL)

    2017-06-28

    Jun 28, 2017 ... KMs are rare, and there are few cases in the literature. The etiology of the ... KEYWORDS: Dentigerous cyst, fourth molars, kissing molar, mucopolysaccharidosis ... or after the tooth eruption.[8,9] Thus, it is not clear whether.

  12. Kissing molars extraction: Case series and review of the literature.

    Science.gov (United States)

    Arjona-Amo, Manuel; Torres-Carranza, Eusebio; Batista-Cruzado, Antonio; Serrera-Figallo, Maria-Angeles; Crespo-Torres, Santos; Belmonte-Caro, Rodolfo; Albisu-Andrade, Claudio; Torres-Lagares, Daniel; Gutiérrez-Pérez, José-Luis

    2016-02-01

    Kissing molars are a very rare form of inclusion defined as molars included in the same quadrant, with occlusal surfaces contacting each other within a single dental follicle. We present four cases of this pathology: a 35 year-old male, referred to the Oral and Maxillofacial Surgery Department of the Hospital Virgen del Rocio in Seville, and three females of 24, 26, and 31 years, all of which had kissing molars that were treated by tooth extraction. We have found only 10 cases published in the medical literature in which this type of inclusion is briefly described, none of which elaborate on the surgical technique employed. In these cases, the indication for surgery is established when there is a history of recurring infections or cystic lesions associated with dental inclusions. The extraction of kissing molars requires an exhaustive comprehension of the anatomy of the region involved, sufficiently developed surgical abilities, and an extensive planning process. Impacted molar, kissing molar, surgical extraction.

  13. Kissing aneurysms of the distal anterior cerebral artery.

    Science.gov (United States)

    Choi, Chan-Young; Han, Seong-Rok; Yee, Gi-Taek; Lee, Chae-Heuck

    2011-02-01

    Kissing aneurysms, a particular type of multiple aneurysm are rare. A kissing aneurysms was identified at the distal anterior cerebral artery (ACA) in a 59-year-old male patient diagnosed with subarachnoid hemorrhage (SAH). The use of three-dimensional intracranial CT angiograms revealed that kissing aneurysms (that is, an aneurysm with a bilateral symmetrical mirror image) were located at the distal ACA and diffuse SAH in basal, sylvian, and interhemispheric cisterns. Both conventional carotid angiograms showed that both distal ACA aneurysms were seen separately on both internal carotid angiograms. Two aneurysms were observed simultaneously on carotid compression of either side. Some particular cautions required in diagnosing and treating kissing aneurysms are discussed, together with a literature review. Copyright © 2010. Published by Elsevier Ltd.

  14. Expression of neurokinin B/NK3 receptor and kisspeptin/KISS1 receptor in human granulosa cells.

    Science.gov (United States)

    García-Ortega, J; Pinto, F M; Fernández-Sánchez, M; Prados, N; Cejudo-Román, A; Almeida, T A; Hernández, M; Romero, M; Tena-Sempere, M; Candenas, L

    2014-12-01

    Are neurokinin B (NKB), NK3 receptor (NK3R), kisspeptin (KISS1) and kisspeptin receptor (KISS1R) expressed in human ovarian granulosa cells? The NKB/NK3R and kisspeptin/KISS1R systems are co-expressed and functionally active in ovarian granulosa cells. The NKB/NK3R and KISS1/KISS1R systems are essential for reproduction. In addition to their well-recognized role in hypothalamic neurons, these peptide systems may contribute to the control of fertility by acting directly on the gonads, but such a direct gonadal role remains largely unknown. This study analyzed matched mural granulosa cells (MGCs) and cumulus cells (CCs) collected from preovulatory follicles of oocyte donors at the time of oocyte retrieval. The samples were provided by 56 oocyte donor women undergoing ovarian stimulation treatment. Follicular fluid samples containing MGCs and cumulus-oocyte complexes were collected after transvaginal ultrasound-guided oocyte retrieval. RT-PCR, quantitative real-time PCR, immunocytochemistry and western blot were used to investigate the pattern of expression of the NKB/NK3R and KISS/KISS1R systems in MGCs and CCs. Intracellular free Ca(2+) levels, [Ca(2+)]i, in MGCs after exposure to NKB or KISS1, in the presence or not of tachykinin receptor antagonists, were also measured. NKB/NK3R and KISS1/KISS1R systems were expressed, at the mRNA and protein levels, in MGCs and CCs, with significantly higher expression in CCs. Kisspeptin increased the [Ca(2+)]i in the cytosol of human MGCs while exposure to NKB failed to induce any change in [Ca(2+)]i. However, the [Ca(2+)]i response to kisspeptin was reduced in the presence of NKB. The inhibitory effect of NKB was only partially mimicked by the NK3R agonist, senktide and marginally suppressed by the NK3R-selective antagonist SB 222200. Yet, a cocktail of antagonists selective for the NK1, NK2 and NK3 receptors blocked the effect of NKB. The granulosa and cumulus cells were obtained from oocyte donors undergoing ovarian

  15. Gravity's kiss the detection of gravitational waves

    CERN Document Server

    Collins, Harry

    2017-01-01

    Scientists have been trying to confirm the existence of gravitational waves for fifty years. Then, in September 2015, came a "very interesting event" (as the cautious subject line in a physicist's email read) that proved to be the first detection of gravitational waves. In Gravity's Kiss, Harry Collins -- who has been watching the science of gravitational wave detection for forty-three of those fifty years and has written three previous books about it -- offers a final, fascinating account, written in real time, of the unfolding of one of the most remarkable scientific discoveries ever made. Predicted by Einstein in his theory of general relativity, gravitational waves carry energy from the collision or explosion of stars. Dying binary stars, for example, rotate faster and faster around each other until they merge, emitting a burst of gravitational waves. It is only with the development of extraordinarily sensitive, highly sophisticated detectors that physicists can now confirm Einstein's prediction. This is...

  16. Programmable Current-Mode Universal Active Filters Employing Current Conveyors

    OpenAIRE

    Muhammad Taher Abuelma'atti

    1998-01-01

    Novel current-mode active filter circuits using current-conveyors are presented. The proposed circuits can realize all standard second-order filter functions. The circuits enjoy high output impedances and can, therefore, be easily cascaded to produce higher order filters. Moreover, the realized filter function can be easily programmed using at most a 13-bit digital number without changing the circuit topology.

  17. Non-Destructive Evaluation of Kissing Bonds using Local Defect Resonance (LDR) Spectroscopy: A Simulation Study

    Science.gov (United States)

    Delrue, S.; Tabatabaeipour, M.; Hettler, J.; Van Den Abeele, K.

    With the growing demand from industry to optimize and further develop existing Non-Destructive Testing & Evaluation (NDT&E) techniques or new methods to detect and characterize incipient damage with high sensitivity and increased quality, ample efforts have been devoted to better understand the typical behavior of kissing bonds, such as delaminations and cracks. Recently, it has been shown experimentally that the nonlinear ultrasonic response of kissing bonds could be enhanced by using Local Defect Resonance (LDR) spectroscopy. LDR spectroscopy is an efficient NDT technique that takes advantage of the characteristic fre- quencies of the defect (defect resonances) in order to provide maximum acoustic wave-defect interaction. In fact, for nonlinear methodologies, the ultrasonic excitation of the sample should occur at either multiples or integer ratios of the characteristic defect resonance frequencies, in order to obtain the highest signal-to-noise response in the nonlinear LDR spectroscopy. In this paper, the potential of using LDR spectroscopy for the detection, localization and characterization of kissing bonds is illustrated using a 3D simulation code for elastic wave propagation in materials containing closed but dynamically active cracks or delaminations. Using the model, we are able to define an appropriate method, based on the Scaling Subtraction Method (SSM), to determine the local defect resonance frequencies of a delamination in a composite plate and to illustrate an increase in defect nonlinearity due to LDR. The simulation results will help us to obtain a better understanding of the concept of LDR and to assist in the further design and testing of LDR spectroscopy for the detection, localization and characterization of kissing bonds.

  18. Effectiveness of a physical activity programme based on the Pilates method in pregnancy and labour.

    Science.gov (United States)

    Rodríguez-Díaz, Luciano; Ruiz-Frutos, Carlos; Vázquez-Lara, Juana María; Ramírez-Rodrigo, Jesús; Villaverde-Gutiérrez, Carmen; Torres-Luque, Gema

    To assess the effectiveness and safety of a physical activity programme based on use of the Pilates method, over eight weeks in pregnant women, on functional parameters, such as weight, blood pressure, strength, flexibility and spinal curvature, and on labour parameters, such as, type of delivery, episiotomy, analgesia and newborn weight. A randomized clinical trial was carried out on pregnant women, applying a programme of physical activity using the Pilates method, designed specifically for this population. A sample consisting of a total of 105 pregnant women was divided into two groups: intervention group (n=50) (32.87±4.46 years old) and control group (n=55) (31.52±4.95 years old). The intervention group followed a physical activity programme based on the Pilates method, for 2 weekly sessions, whereas the control group did not follow the program. Significant improvements (p<0.05) in blood pressure, hand grip strength, hamstring flexibility and spinal curvature, in addition to improvements during labour, decreasing the number of Caesareans and obstructed labour, episiotomies, analgesia and the weight of the newborns were found at the end of the intervention. A physical activity programme of 8 weeks based on the Pilates method improves functional parameters in pregnant women and benefits delivery. Copyright © 2017 Elsevier España, S.L.U. All rights reserved.

  19. Kissing reduces allergic skin wheal responses and plasma neurotrophin levels.

    Science.gov (United States)

    Kimata, Hajime

    2003-11-01

    The effect of kissing on allergen-induced skin wheal responses and plasma neurotrophin levels were studied in 30 normal subjects, 30 patients with allergic rhinitis (AR), and 30 patients with atopic dermatitis (AD). All of the patients with AR or AD are allergic to house dust mite (HDM) and Japanese cedar pollen (JCP). They are all Japanese and they do not kiss habitually. The subject kissed freely during 30 min with their lover or spouse alone in a room with closed doors while listening to soft music. Before and after kissing, skin prick tests were performed using commercial HDM allergen, JCP allergen, as well as histamine and control solution, and wheal responses were measured. Simultaneously, plasma levels of neurotrophin, including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and -4 (NT-4) were measured. Kissing significantly reduced wheal responses induced by HDM and JCP, but not by histamine, and decreased plasma levels of NGF, BDNF, NT-3, and NT-4 in patients with AR or AD, while it failed to do so in normal subjects. These finding indicate that kissing have some implication in the study of neuroimmunology in allergic patients.

  20. Evaluation of physical activity programmes for elderly people - a descriptive study using the EFQM' criteria

    Directory of Open Access Journals (Sweden)

    Santos Rute

    2011-02-01

    Full Text Available Abstract Background In the past years, there has been a growing concern in designing physical activity (PA programmes for elderly people, because evidence suggests that such health promotion interventions may reduce the deleterious effects of the ageing process. Quality is an important issue when designing a PA programme for older people. Some studies support the Excellence Model of the European Foundation for Quality Management (EFQM as an operational framework for evaluating the quality of an organization. Within this context, the aim of this study was to characterize the quality management models of the PA programmes developed by Portuguese Local Administration to enhance quality of life for elderly people, according to the criteria of the EFQM Excellence Model. Methods A methodological triangulation was conducted in 26 PA programmes using questionnaire surveys, semi-structured interviews and document analysis. We used standard approaches to the statistical analysis of data including frequencies and percentages for the categorical data. Results Results showed that Processes (65,38%, Leadership (61,03%, Customer results (58,46 and People (51,28% had high percentage occurrences of quality practices. In contrast, Partnerships and resources (45,77%, People results (41,03%, Policy and strategy (37,91%, Key performance results (19,23% and Society results (19,23% had lower percentage occurrences. Conclusions Our findings suggest that although there are some good practices in PA programmes, there are still relevant areas that require improvement.

  1. Evaluation of physical activity programmes for elderly people - a descriptive study using the EFQM' criteria.

    Science.gov (United States)

    Marques, Ana I; Rosa, Maria J; Soares, Pedro; Santos, Rute; Mota, Jorge; Carvalho, Joana

    2011-02-21

    In the past years, there has been a growing concern in designing physical activity (PA) programmes for elderly people, because evidence suggests that such health promotion interventions may reduce the deleterious effects of the ageing process. Quality is an important issue when designing a PA programme for older people. Some studies support the Excellence Model of the European Foundation for Quality Management (EFQM) as an operational framework for evaluating the quality of an organization. Within this context, the aim of this study was to characterize the quality management models of the PA programmes developed by Portuguese Local Administration to enhance quality of life for elderly people, according to the criteria of the EFQM Excellence Model. A methodological triangulation was conducted in 26 PA programmes using questionnaire surveys, semi-structured interviews and document analysis. We used standard approaches to the statistical analysis of data including frequencies and percentages for the categorical data. Results showed that Processes (65,38%), Leadership (61,03%), Customer results (58,46) and People (51,28%) had high percentage occurrences of quality practices. In contrast, Partnerships and resources (45,77%), People results (41,03%), Policy and strategy (37,91%), Key performance results (19,23%) and Society results (19,23%) had lower percentage occurrences. Our findings suggest that although there are some good practices in PA programmes, there are still relevant areas that require improvement.

  2. Utilisation of Wearable Computing for Space Programmes Test Activities Optimasation

    Science.gov (United States)

    Basso, V.; Lazzari, D.; Alemanni, M.

    2004-08-01

    New technologies are assuming a relevant importance in the Space business domain also in the Assembly Integration and Test (AIT) activities allowing process optimization and capability that were unthinkable only few years ago. This paper has the aim to describe Alenia Spazio (ALS) gained experience on the remote interaction techniques as a results of collaborations established both on European Communities (EC) initiatives, with Alenia Aeronautica (ALA) and Politecnico of Torino (POLITO). The H/W and S/W components performances increase and costs reduction due to the home computing massive utilization (especially demanded by the games business) together with the network technology possibility (offered by the web as well as the hi-speed links and the wireless communications) allow today to re-think the traditional AIT process activities in the light of the multimedia data exchange: graphical, voice video and by sure more in the future. Aerospace business confirm its innovation vocation which in the year '80 represents the cradle of the CAD systems and today is oriented to the 3D data visualization/ interaction technologies and remote visualisation/ interaction in collaborative way on a much more user friendly bases (i.e. not for specialists). Fig. 1 collects AIT extended scenario studied and adopted by ALS in these years. ALS experimented two possibilities of remote visualization/interaction: Portable [e.g. Fig.2 Personal Digital Assistant (PDA), Wearable] and walls (e.g.VR-Lab) screens as both 2D/3D visualisation and interaction devices which could support many types of traditional (mainly based on EGSE and PDM/CAD utilisation/reports) company internal AIT applications: 1. design review support 2. facility management 3. storage management 4. personnel training 5. integration sequences definition 6. assembly and test operations follow up 7. documentation review and external access to AIT activities for remote operations (e.g. tele-testing) EGSE Portable Clean room

  3. Evaluation of physical activity programmes for the elderly - exploring the lessons from other sectors and examining the general characteristics of the programmes

    Directory of Open Access Journals (Sweden)

    Clara-Santos Paula

    2011-09-01

    Full Text Available Abstract Background In Portugal, there are several physical activity (PA programmes for elderly people developed by the local government. The importance of these programmes has been increasing since the evidence has shown that this type of health promotion interventions may reduce the deleterious effects of the ageing process. However, no study has already identified the general characteristics of these programmes nor if they use any scheme to assess the quality of the service provided. A widely-used scheme is the EFQM Excellence Model, which will be in the core of our present work. Thus, the main aims of this preliminary study were 1 to identify the general characteristics of the PA programmes developed by the Portuguese Local Public Administration 2 to determine the extent of implementation of quality initiatives in these programmes. Methods Data were collected by an on-line questionnaire sent to all Continental Municipalities (n = 278. Categorical data were expressed as absolute counts and percentages. Continuous data were expressed as the mean and SD. An open-ended question was analysed using qualitative content analysis with QSR NVivo software. Associations between categorical variables were tested by the use of contingency tables and the calculation of chi-square tests. Significance level was set at p ≤ 0.05. Results Results showed: i a total of 125 PA programmes were identified in the 18 districts of the Portugal mainland; ii the main goal of the majority (95.2% was the participants' health promotion; iii different characteristics of the programmes were found according to different regions of the country; iv certain characteristics of the programmes were associated to the existence of other features; v only one PA programme developed quality initiatives. Conclusions In conclusion, although there are many PA programmes for elderly people spread throughout the country, aiming at improving the health of participants, the overwhelming

  4. Oncogenic programmes and Notch activity: an 'organized crime'?

    Science.gov (United States)

    Dominguez, Maria

    2014-04-01

    The inappropriate Notch signalling can influence virtually all aspect of cancer, including tumour-cell growth, survival, apoptosis, angiogenesis, invasion and metastasis, although it does not do this alone. Hence, elucidating the partners of Notch that are active in cancer is now the focus of much intense research activity. The genetic toolkits available, coupled to the small size and short life of the fruit fly Drosophila melanogaster, makes this an inexpensive and effective animal model, suited to large-scale cancer gene discovery studies. The fly eye is not only a non-vital organ but its stereotyped size and disposition also means it is easy to screen for mutations that cause tumours and metastases and provides ample opportunities to test cancer theories and to unravel unanticipated nexus between Notch and other cancer genes, or to discover unforeseen Notch's partners in cancer. These studies suggest that Notch's oncogenic capacity is brought about not simply by increasing signal strength but through partnerships, whereby oncogenes gain more by cooperating than acting individually, as in a ring 'organized crime'.

  5. Hydrogen activities in the European Union work-programme. Paper

    Energy Technology Data Exchange (ETDEWEB)

    Bahbout, A.; Tartaglia, G.P.; Buenger, U.

    2000-07-01

    Looking at some of the national and international developments in hydrogen technology it becomes clear which important contributions the hydrogen technology oriented activities of the EU have helped to prepare and trigger: (a) Transport Energy Strategy (TES): This initiative of 7 major German automobile and mineral oilcompanies is aimed at an industrial consensus on one or two gasoline alternative fuels, which are to be presented to the German Ministry of Transport. An intermediate trend is that hydrogen may become the fuel of choice. (b) BMW: The Bayerischen Motorenwerke have already very early exposed themselves to the vehicle and component development of hydrogen as a vehicle fuel, focussing on a strategy from CNG to LNG and LH{sub 2}. (c) Opel and GM: Opel has recently announced they have chosen hydrogen as the primary long term fuel for their fuel cell vehicles to be commercialized starting in 2004. (d) CFCP: The California Fuel Cell Partnership with partners from industry and politics has announced they are preparing the installation of hydrogen fuel stations aas well as 20-25 fuel cell buses and 30 passenger cars, mainly operated with hydrogen. (e) NEDO: The New Energy and Industrial Technology Development Organization (NEDO) of Japan has announced they are going to build hydrogen pilot refueling stations 18 months ahead of the original schedule to reduce the first-to-market-time. (f) Norway: A study group of Norwegian industry and institutes has carried out a comprehensive study for the Research Ministry on further R and D areas which should be intensified in a national strategy to be prepared for an international hydrogen energy system [SINTEF, 00]. (g) German Greens: The German ecologist party ''Greens'' has announced last week a shift from an anti-car lobbying to fostering greener cars, focussing on renewable hydrogen as a clean fuel. (h) Linde: The largest European Technical Gas Company has announced recently they will strategically

  6. Sport activity and eating habits of people who were attending special obesity treatment programme.

    Science.gov (United States)

    Videmsek, Mateja; Stihec, Joze; Karpljuk, Damir; Starman, Anja

    2008-09-01

    The aims of the study were to analyse the sport activity and eating habits of obese people in their childhood and adulthood. The research was underpinned by a survey questionnaire containing 37 variables which was completed by 71 people attending the obesity programme. The frequencies and contingency tables were calculated, whereas statistical significance was established at a 5% significance level. The analysis of the results showed that more than one-half of the survey respondents joined the obesity programme primarily for reasons of health and well-being. Most obese people did not engage in any organised sport activity in their childhood, nor did most of their parents. The respondents practiced sport in their childhood to a greater extent if their parents were also physically active and if they guided and encouraged their children. No less than one-third of the respondents were overweight in their childhood, of whom two-thirds did not participate in any organised sport activity. The majority of the respondents (85.9%) are currently engaged in an organised sport activity in their adulthood, mainly due to their participation in the weight reduction programme; most of them practice sport twice a week. Their eating habits are encouraging; the share of skipped meals is considerably lower and practically negligible compared to that in childhood. It has to be emphasized that most of them are of opinion that obese people have difficulties finding expert information on obesity, nutrition and sport activities as well as weight management centers and institutions.

  7. Sex Differences in Romantic Kissing among College Students: An Evolutionary Perspective

    Directory of Open Access Journals (Sweden)

    Susan M. Hughes

    2007-07-01

    Full Text Available This study provides a descriptive account of kissing behavior in a large sample of undergraduate college students and considers kissing in the context of both short-term and long-term mating relationships. Kissing was examined as a mate assessment device, a means of promoting pair bonds, and a means of inducing sexual arousal and receptivity. A total 1,041 college students completed one of three questionnaires measuring kissing preferences, attitudes, styles, and behaviors. Results showed that females place more importance on kissing as a mate assessment device and as a means of initiating, maintaining, and monitoring the current status of their relationship with a long-term partner. In contrast, males place less importance on kissing, especially with short-term partners, and appear to use kissing to increase the likelihood of having sex. The results suggest that kissing may play an important role as an adaptive courtship/mating ritual.

  8. Reason and Condition for Mode Kissing in MASW Method

    Science.gov (United States)

    Gao, Lingli; Xia, Jianghai; Pan, Yudi; Xu, Yixian

    2016-05-01

    Identifying correct modes of surface waves and picking accurate phase velocities are critical for obtaining an accurate S-wave velocity in MASW method. In most cases, inversion is easily conducted by picking the dispersion curves corresponding to different surface-wave modes individually. Neighboring surface-wave modes, however, will nearly meet (kiss) at some frequencies for some models. Around the frequencies, they have very close roots and energy peak shifts from one mode to another. At current dispersion image resolution, it is difficult to distinguish different modes when mode-kissing occurs, which is commonly seen in near-surface earth models. It will cause mode misidentification, and as a result, lead to a larger overestimation of S-wave velocity and error on depth. We newly defined two mode types based on the characteristics of the vertical eigendisplacements calculated by generalized reflection and transmission coefficient method. Rayleigh-wave mode near the kissing points (osculation points) change its type, that is to say, one Rayleigh-wave mode will contain different mode types. This mode type conversion will cause the mode-kissing phenomenon in dispersion images. Numerical tests indicate that the mode-kissing phenomenon is model dependent and that the existence of strong S-wave velocity contrasts increases the possibility of mode-kissing. The real-world data shows mode misidentification caused by mode-kissing phenomenon will result in higher S-wave velocity of bedrock. It reminds us to pay attention to this phenomenon when some of the underground information is known.

  9. Process evaluation of a school based physical activity related injury prevention programme using the RE-AIM framework

    Directory of Open Access Journals (Sweden)

    van Mechelen Willem

    2010-11-01

    Full Text Available Abstract Background In general, only information regarding the effectiveness of an intervention programme is ever published. However, in recent years evaluating the translatability and feasibility of an intervention programme has become more important. Therefore, this paper presents the results of the evaluation of the iPlay programme aimed at preventing physical activity related injuries in primary school children. Methods The iPlay programme targeted injuries gained through physical activity, and consisted of a teacher's manual, informative newsletters and posters, a website, and set exercises to be carried out during physical education (PE classes. In order to evaluate the iPlay programme for translatability and feasibility, teachers, children and parents who participated in the iPlay programme filled out a questionnaire The objective of this study is to describe the results of the process-evaluation of the iPlay programme based on the five dimensions of the RE-AIM framework. Results The results showed that the participation rate of the children was 100% (reach. Nine percent of the schools who were invited to take part were willing to participate in the study (adoption rate. Teachers stated that they implemented the different elements of the programme partly as intended (implementation. The percentage of children and parents who followed the programme was less than expected. In addition, 52% of the teachers indicated that the current iPlay programme could become standard practice in their teaching routine (maintenance. Conclusion The iPlay programme is a first start in the prevention of physical activity related injuries in children, but further improvements need to be made to the programme on the basis of this process evaluation. Trial registration ISRCTN78846684; http://www.controlled-trials.com

  10. Applying a nonlinear, pitch-catch, ultrasonic technique for the detection of kissing bonds in friction stir welds.

    Science.gov (United States)

    Delrue, Steven; Tabatabaeipour, Morteza; Hettler, Jan; Van Den Abeele, Koen

    2016-05-01

    Friction stir welding (FSW) is a promising technology for the joining of aluminum alloys and other metallic admixtures that are hard to weld by conventional fusion welding. Although FSW generally provides better fatigue properties than traditional fusion welding methods, fatigue properties are still significantly lower than for the base material. Apart from voids, kissing bonds for instance, in the form of closed cracks propagating along the interface of the stirred and heat affected zone, are inherent features of the weld and can be considered as one of the main causes of a reduced fatigue life of FSW in comparison to the base material. The main problem with kissing bond defects in FSW, is that they currently are very difficult to detect using existing NDT methods. Besides, in most cases, the defects are not directly accessible from the exposed surface. Therefore, new techniques capable of detecting small kissing bond flaws need to be introduced. In the present paper, a novel and practical approach is introduced based on a nonlinear, single-sided, ultrasonic technique. The proposed inspection technique uses two single element transducers, with the first transducer transmitting an ultrasonic signal that focuses the ultrasonic waves at the bottom side of the sample where cracks are most likely to occur. The large amount of energy at the focus activates the kissing bond, resulting in the generation of nonlinear features in the wave propagation. These nonlinear features are then captured by the second transducer operating in pitch-catch mode, and are analyzed, using pulse inversion, to reveal the presence of a defect. The performance of the proposed nonlinear, pitch-catch technique, is first illustrated using a numerical study of an aluminum sample containing simple, vertically oriented, incipient cracks. Later, the proposed technique is also applied experimentally on a real-life friction stir welded butt joint containing a kissing bond flaw. Copyright © 2016

  11. New and recently finalised activities within the NKS Programmes for Nordic cooperation on nuclear reactor safety and emergency preparedness

    DEFF Research Database (Denmark)

    Andgren, Karin; Andersson, Kasper Grann; Magnússon, Sigurður M.

    2015-01-01

    Over the years, NKS has provided funding for hundreds of research activities in fields comprising reactor safety, decommissioning, nuclear and radiological emergency preparedness, and management of radioactive waste. Advanced technologies and methods developed under the NKS framework have been used...... within the Nordic countries as well as internationally. Two programme areas are defined under the NKS platform: The NKS-R programme on nuclear reactor safety and the NKS-B programme on emergency preparedness. Three articles, giving an introduction to NKS and its two programmes, were published...

  12. LIN28B, LIN28A, KISS1, and KISS1R in idiopathic central precocious puberty

    Directory of Open Access Journals (Sweden)

    Koivu Rosanna

    2011-09-01

    Full Text Available Abstract Background Pubertal timing is a strongly heritable trait, but no single puberty gene has been identified. Thus, the genetic background of idiopathic central precocious puberty (ICPP is poorly understood. Overall, the genetic modulation of pubertal onset most likely arises from the additive effect of multiple genes, but also monogenic causes of ICPP probably exist, as cases of familial ICPP have been reported. Mutations in KISS1 and KISSR, coding for kisspeptin and its receptor, involved in GnRH secretion and puberty onset, have been suggested causative for monogenic ICPP. Variation in LIN28B was associated with timing of puberty in genome-wide association (GWA studies. LIN28B is a human ortholog of the gene that controls, through microRNAs, developmental timing in C. elegans. In addition, Lin28a transgenic mice manifest the puberty phenotypes identified in the human GWAS. Thus, both LIN28B and LIN28A may have a role in pubertal development and are good candidate genes for monogenic ICPP. Methods Thirty girls with ICPP were included in the study. ICPP was defined by pubertal onset before 8 yrs of age, and a pubertal LH response to GnRH testing. The coding regions of LIN28B, LIN28A, KISS1, and KISS1R were sequenced. The missense change in LIN28B was also screened in 132 control subjects. Results No rare variants were detected in KISS1 or KISS1R in the 30 subjects with ICPP. In LIN28B, one missense change, His199Arg, was found in one subject with ICPP. However, this variant was also detected in one of the 132 controls. No variation in LIN28A was found. Conclusions We did not find any evidence that mutations in LIN28B or LIN28A would underlie ICPP. In addition, we confirmed that mutations in KISS1 and KISS1R are not a common cause for ICPP.

  13. LIN28B, LIN28A, KISS1, and KISS1R in idiopathic central precocious puberty.

    Science.gov (United States)

    Tommiska, Johanna; Sørensen, Kaspar; Aksglaede, Lise; Koivu, Rosanna; Puhakka, Lea; Juul, Anders; Raivio, Taneli

    2011-09-22

    Pubertal timing is a strongly heritable trait, but no single puberty gene has been identified. Thus, the genetic background of idiopathic central precocious puberty (ICPP) is poorly understood. Overall, the genetic modulation of pubertal onset most likely arises from the additive effect of multiple genes, but also monogenic causes of ICPP probably exist, as cases of familial ICPP have been reported. Mutations in KISS1 and KISSR, coding for kisspeptin and its receptor, involved in GnRH secretion and puberty onset, have been suggested causative for monogenic ICPP. Variation in LIN28B was associated with timing of puberty in genome-wide association (GWA) studies. LIN28B is a human ortholog of the gene that controls, through microRNAs, developmental timing in C. elegans. In addition, Lin28a transgenic mice manifest the puberty phenotypes identified in the human GWAS. Thus, both LIN28B and LIN28A may have a role in pubertal development and are good candidate genes for monogenic ICPP. Thirty girls with ICPP were included in the study. ICPP was defined by pubertal onset before 8 yrs of age, and a pubertal LH response to GnRH testing. The coding regions of LIN28B, LIN28A, KISS1, and KISS1R were sequenced. The missense change in LIN28B was also screened in 132 control subjects. No rare variants were detected in KISS1 or KISS1R in the 30 subjects with ICPP. In LIN28B, one missense change, His199Arg, was found in one subject with ICPP. However, this variant was also detected in one of the 132 controls. No variation in LIN28A was found. We did not find any evidence that mutations in LIN28B or LIN28A would underlie ICPP. In addition, we confirmed that mutations in KISS1 and KISS1R are not a common cause for ICPP.

  14. Kissing nevus of the penis. Report of two cases and review of the literature.

    Science.gov (United States)

    Wang, Songting; Zhou, Mingshu; Qiao, Jianjun

    2014-01-01

    Kissing nevus is a curious type of nevus that was first described on the eyelids and rarely described on the penis. We report two cases of kissing nevus of the penis and review previously reported cases. The lesions of the kissing nevus of the penis showed characteristic mirror-image symmetry relative to the coronal sulcus. On histopathology, the lesion showed a compound nevus.

  15. Intraarterial chemotherapy for kissing macula tumors in retinoblastoma.

    Science.gov (United States)

    Abramson, David H; Marr, Brian P; Brodie, Scott; Dunkel, Ira J; Gobin, Pierre Y

    2012-01-01

    To describe the management of kissing macula retinoblastoma tumors treated with intraarterial chemotherapy. Superselective infusion of chemotherapy (combinations of melphalan, topotecan, and carboplatin) into the ophthalmic artery of children with retinoblastoma who had kissing macula tumors was administered on an outpatient basis. Results were assessed with indirect ophthalmoscopy, RetCam digital photography, B-scan ultrasonography, and electroretinograms. Three children with bilateral retinoblastoma (aged 6.1, 7, and 16 months) and kissing macula tumors in an eye were treated. All children are alive without the need for removal of the eye, systemic chemotherapy, or external beam irradiation. In each case, the kissing tumors retracted, leaving an anatomically normal fovea. Final 30-Hz flicker electroretinograms were 93.9, 65, and 63.9 μV (under anesthesia). There were no significant systemic toxicities. Superselective infusion of chemotherapy by means of the ophthalmic artery of young children with kissing macula tumors resulted in prompt shrinkage of tumors away from the fovea. There were no systemic side effects.

  16. Malignant transformation of kissing nevus- a rare entity.

    Science.gov (United States)

    Kharel Sitaula, R; Batta, S; Shrestha, G B; Shrestha, J K

    2012-01-01

    Kissing nevus is a congenital nevus in adjacent parts of the eyelids. Malignant transformation of kissing or divided nevi of the eyelids is rarely described. To report a very rare case of malignant transformation of kissing nevus with ocular and extraocular spread. A 57- year- old man with 6/6 visual acuity in both eyes presented with a kissing nevus present since birth in right upper and lower eyelids which had a slow growth phase. The upper lid in the area of the nevus was thickened with a 20x12x15 mm black pigmented crusted hemorrhagic nodular lesions. The lower lid had a 6 mm black pigmented ulcerated lesion over the pre-existing nevus in the lateral third of the lid with full thickness infiltration. Another 5x4 mm pigmented lesion over the lower medial lid margin with a thickness of about 3 mm extended to the conjunctival side of the lower lid. Right sided pre-auricular and sub-maxillary nodes were palpable. A biopsy of tissue samples from the eyelid and pre-auricular nodes were consistent with malignant melanoma. Malignant transformation of kissing nevus is rare. It can spread to the conjunctiva,pre-auricular and sub-mandibular lymphnodes. © NEPjOPH.

  17. Ictal kissing: Electroclinical features of an unusual ictal phenomenon.

    Science.gov (United States)

    Özkara, Çiğdem; Taşkıran, Emine; Çarpraz, İrem Yıldırım; Bilir, Erhan; Demir, Aylin Bican; Bora, İbrahim; Aydoğdu, İbrahim; Delil, Şakir; Chassoux, Francine

    2016-11-01

    The study aimed to describe the electroclinical features of ictal kissing, an unusual behavior that may occur during focal seizures. Twenty-five patients collected from four epilepsy centers and previously published reports were reviewed for their demographic, clinical, and electrophysiological features. Sixteen of 25 patients were female. The mean age was 32.9 years (9.9-51 years) and the average age at seizure onset was 14.5 years. All seizures were localized to the temporal lobe (TL) and lateralized to right side in 17 patients, left side in 2 patients, and unclear in 6 patients. A total of 55 ictal video electroencephalograms (EEGs) were analyzed. There were other symptoms such as speaking, spitting, hugging, and oral and upper-extremity automatisms along with different types of ictal kissing behavior during the seizures. The median duration of all seizures was 106.7±73.73s. Kissing occurred at a median time of 71s (1-95s) after the onset of seizure, and ictal epileptiform discharges usually involved TL during kissing episode. Ictal kissing is mainly associated with right TL and female gender, although association with left TL may also occurs, possibly related to the circuits involving temporo-insular structures. Copyright © 2016 British Epilepsy Association. Published by Elsevier Ltd. All rights reserved.

  18. Structural insights into substrate recognition by the Neurospora Varkud satellite ribozyme: importance of U-turns at the kissing-loop junction.

    Science.gov (United States)

    Bouchard, Patricia; Legault, Pascale

    2014-01-14

    Substrate recognition by the Neurospora Varkud satellite ribozyme depends on the formation of a magnesium-dependent kissing-loop interaction between the stem-loop I (SLI) substrate and stem-loop V (SLV) of the catalytic domain. From mutagenesis studies, it has been established that this I/V kissing-loop interaction involves three Watson-Crick base pairs and is associated with a structural rearrangement of the SLI substrate that facilitates catalysis. Here, we report the NMR structural characterization of this I/V kissing-loop using isolated stem-loops. NMR studies were performed on different SLI/SLV complexes containing a common SLV and shiftable, preshifted, or double-stranded SLI variants. These studies confirm the presence of three Watson-Crick base pairs at the kissing-loop junction and provide evidence for the structural rearrangement of shiftable SLI variants upon SLV binding. NMR structure determination of an SLI/SLV complex demonstrates that both the SLI and SLV loops adopt U-turn structures, which facilitates intermolecular Watson-Crick base pairing. Several other interactions at the I/V interface, including base triples and base stacking, help create a continuously stacked structure. These NMR studies provide a structural basis to understand the stability of the I/V kissing-loop interaction and lead us to propose a kinetic model for substrate activation in the VS ribozyme.

  19. Sport Activity and Eating Habits of People Who Were Attending Special Obesity Treatment Programme

    OpenAIRE

    Videmšek, Mateja; Štihec, Jože; Karpljuk, Damir; Starman, Anja

    2008-01-01

    The aims of the study were to analyse the sport activity and eating habits of obese people in their childhood and adulthood. The research was underpinned by a survey questionnaire containing 37 variables which was completed by 71 people attending the obesity programme. The frequencies and contingency tables were calculated, whereas statistical significance was established at a 5% significance level. The analysis of the results showed that more than one-half of the survey responden...

  20. Kissing balloon inflation in percutaneous coronary interventions.

    Science.gov (United States)

    Sgueglia, Gregory A; Chevalier, Bernard

    2012-08-01

    Bifurcation lesions are the most frequently approached complex coronary lesions in everyday interventional practice. Bifurcations complexity relies essentially on their very specific anatomy that is imperfectly handled by current coronary devices and, despite dedicated techniques and drug-eluting stents, percutaneous coronary interventions directed toward the treatment of bifurcations are technically demanding and require proper execution. Kissing balloon (KB) inflation was the first specific bifurcation technique to have been developed for percutaneous bifurcation interventions and continues to currently play an important role. Indeed, KB has been proposed to optimize stent apposition, improve side branch access while correcting stent deformation or distortion. Over the years, the KB technique has been deeply investigated by many different methods, from bench testing and computer simulations to in vivo intravascular imaging and clinical studies, producing a large amount of data pointing out the benefits and limitations of the technique. We sought to provide here a comprehensive overview of all those aspects. Copyright © 2012 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved.

  1. Planetary protection R&D activities in the ESA exploration programme

    Science.gov (United States)

    Kminek, G.

    Since the begin of the Aurora exploration programme in 2001 the Human Spaceflight Microgravity and Exploration Directorate HME of ESA has invested in research and development activities related to planetary protection Some of these activities are focused on the recently approved ExoMars mission others are applicable to Mars missions in general including MSR the technology development of the latter one being part of the exploration core programme The proposed activities have been approved and initiated An overview of the activities and first results will be presented The main activities are begin itemize item Bioburden and Biodiversity evaluation in S C Facilities this activity will cover a period of almost two years and include the standard assay extension of the standard assay culture conditions identification of isolates using 16S rDNA via PCR and test of a rapid spore assay Protocols are developed in coordination with NASA-JPL item Extension of dry heat microbial reduction process to higher temperatures this activity will include a detailed study of the humidity effect on the inactivation kinetics This activity is in coordination with efforts at NASA-JPL item Validation of a dry heat sterilization process item Development of a low-temperature sterilization method the focus of this activity is on vapor hydrogen peroxide item Robotic capabilities for clean AIV AIT item Decontamination of man-rated systems item Definition of functional requirements for a Mars Sample Return Biological Containment Facility end itemize In

  2. Menstrual cycle effects on attitudes toward romantic kissing.

    Science.gov (United States)

    Wlodarski, Rafael; Dunbar, Robin I M

    2013-12-01

    Hormonal changes associated with the human menstrual cycle have been previously found to affect female mate preference, whereby women in the late follicular phase of their cycle (i.e., at higher risk of conception) prefer males displaying putative signals of underlying genetic fitness. Past research also suggests that romantic kissing is utilized in human mating contexts to assess potential mating partners. The current study examined whether women in their late follicular cycle phase place greater value on kissing at times when it might help serve mate assessment functions. Using an international online questionnaire, results showed that women in the follicular phase of their menstrual cycle felt that kissing was more important at initial stages of a relationship than women in the luteal phase of their cycle. Furthermore, it was found that estimated progesterone levels were a significant negative predictor for these ratings.

  3. Active surveillance and control programme for Salmonella Dublin in Cattle

    DEFF Research Database (Denmark)

    Nielsen, Liza Rosenbaum; Rattenborg, Erik

    2011-01-01

    surveillance systems. Register data might be useful for design of effective surveillance programmes for Salmonella Dublin in cattle in the future. Statistical analysis of register-based variables confirmed previously known risk factors for becoming test-positive (i.e. purchase of animals from test......This study illustrates how prevalence and incidence of Salmonella Dublin in cattle has been markedly reduced in dairy herds during active surveillance and a control programme targeting infected herds in Denmark from 2002 to 2010. The results suggest that this might by a good alternative to passive......-positive herds, number of cattle in test-positive neighbouring herds, herd size and season), but also pointed out additional factors affecting the risk of dairy herds changing status from test-negative to test-positive (indicative of new or recurrent infection). Increasing geometric cell count measured through...

  4. A rare case of kissing gastric ulcers caused by trauma.

    Science.gov (United States)

    Zhang, Yan; Zhang, Yafei; Ma, Shiyang; Shi, Haitao; Lu, Xiaolan

    2016-11-01

    We present a rare case of a 32-year-old female with gastric ulcers caused by pressure from trauma. The patient was diagnosed with stress-related acute gastritis by a local hospital and she was discharged 1 week later after her symptoms improved. She was given oral proton pump inhibitors (PPI). However, epigastric pain intensified, so the woman was seen at this Hospital 3 days later. Gastric endoscopy revealed kissing ulcers in the lower body of the stomach. A point worth mentioning is that the kissing gastric ulcers were caused by trauma due to impact with the steering wheel.

  5. Evaluation of Activities in Primary Education Programme with The Teachers’ Views (Sample of Sinop Province

    Directory of Open Access Journals (Sweden)

    Necdet AYKAÇ

    2007-12-01

    Full Text Available This research, aims to investigate the concept of activity on the basis of learning-teaching duration inaccordance with teachers’ views, teachers guide and class materials in the primary education programme put in to practice in 2005- 2006. The research has two subjects. The first one is the quantitative subject aiming to determine the teachers’ views, the second is theoretical subject aiming to analyze the guide books and class metarials. The quantitative research, aiming to determine the teachers’ ideas is consisted of 184 teachers in total in the center and in the central villages of Sinop. The exemplification in this research is occupied by means of 86 teachers applying the teachers’ views determinating scale. In the research as a tool to collect data, determining teachers’ views scale prepared in 5 likert-type scale with 18 articles has been used. Analyzing with SPSS programme, the data gained from the scale has been stated as frequency and percentage. Theoretical dimension examining teachers’ guide and lesson metarials to put the teachers’ views on a objective base, aims to examine teachers guide books and lesson materials analytically and display the base of teachers’ views. It is stated that most of the teachers present their views in that way: They feel themselves insufficent to applythe activities,the physical opportunities of the schools and the facilities are not enough to apply some activities, the classes are crowded, the activities are not student-centered and active teaching methods are not used in the activities.

  6. Effects of a physical activity programme on body perception and composition in overweight adolescents

    Directory of Open Access Journals (Sweden)

    Onofre Ricardo Contreras-Jordán

    2017-03-01

    Full Text Available Background The purpose of this study was to examine the effectiveness of a six months' physical activity programme for overweight and obese adolescents, in order to improve the perception of their own body image and composition between two groups of adolescents, and to check possible gender differences. Aims The aim is to improve the body perception and composition in overweight adolescents with a physical activity programme. Methods It was a quasi-experimental design, with a control group and intervention group. Measures of pre-test and post-test were taken. A total of 38 adolescents that belong to the first cycle of Compulsory Secondary aged 12–15, with a body mass index (BMI higher than the 85th percentile according to the WHO (World Health Organization, participated in the intervention. The anthropometric variables, height, weight, BMI, body fat percentage, and the circumference of the waist, hip, arm and medial calf, were measured pre- and post-treatment. The body image perception was measured using the Gardner test. The experimental group participated in the physical activity programme for a six months period. The physical activity intervention consisted of three sessions of 90 minutes each week in a sports centre. The structure of the sessions consisted of a warm-up (5–10 minutes, a main activity (60–70 minutes, combineding strength and cardiovascular training and a cool-down (7–10 minutes. Results After physical activity intervention the results showed significant improvements in the experimental group compared to the control group in the circumference of the waist, the hip, the arm, the medial calf (p<0.01 and as well as the fat percentage, the percentage of muscle mass (p<0.05. The results showed how the body image distortion presented a significant and negative correlation with the circumference of the waist (p<0.001, of the hips (p<0.01, and of the arm (p<0.001, differences were observed between gender. Conclusion Our study

  7. [Effect of a physical activity programme in patients with fibromyalgia: a systematic review].

    Science.gov (United States)

    Cadenas-Sánchez, Cristina; Ruiz-Ruiz, Jonatan

    2014-12-23

    The aim of this review was to determine what type of physical activity programmes have been developed in patients with fibromyalgia and what are its effects and benefits on the degree of pain and quality of life. The search was performed in MEDLINE, Web of Science and SPORTDiscus databases. The word "fibromyalgia" was always used as a criterion for combined search (using "AND" connector) with physical activity, exercise, physical therapy and training (MeSH terms). Of the 2,531 initial results, 33 papers were selected for review. The studies reviewed focus primarily on dance activities, water activities, multidisciplinary, mind-body work, fitness and stretching. After applying the intervention program, the pain level was reduced between 10 and 44.2%, and the impact of the disease between 5.3 and 17.9%, improving the symptoms of these patients. In conclusion, a multidisciplinary programme (in which physical activity is included) may have positive effects on the quality of life of people with fibromyalgia. Copyright © 2013 Elsevier España, S.L.U. All rights reserved.

  8. Kissing and nanotunneling mediate intermitochondrial communication in the heart.

    Science.gov (United States)

    Huang, Xiaohu; Sun, Lei; Ji, Shuangxi; Zhao, Ting; Zhang, Wanrui; Xu, Jiejia; Zhang, Jue; Wang, Yanru; Wang, Xianhua; Franzini-Armstrong, Clara; Zheng, Ming; Cheng, Heping

    2013-02-19

    Mitochondria in many types of cells are dynamically interconnected through constant fusion and fission, allowing for exchange of mitochondrial contents and repair of damaged mitochondria. However, constrained by the myofibril lattice, the ∼6,000 mitochondria in the adult mammalian cardiomyocyte display little motility, and it is unclear how, if at all, they communicate with each other. By means of target-expressing photoactivatable green fluorescent protein (PAGFP) in the mitochondrial matrix or on the outer mitochondrial membrane, we demonstrated that the local PAGFP signal propagated over the entire population of mitochondria in cardiomyocytes on a time scale of ∼10 h. Two elemental steps of intermitochondrial communications were manifested as either a sudden PAGFP transfer between a pair of adjacent mitochondria (i.e., "kissing") or a dynamic nanotubular tunnel (i.e., "nanotunneling") between nonadjacent mitochondria. The average content transfer index (fractional exchange) was around 0.5; the rate of kissing was 1‰ s(-1) per mitochondrial pair, and that of nanotunneling was about 14 times smaller. Electron microscopy revealed extensive intimate contacts between adjacent mitochondria and elongated nanotubular protrusions, providing a structural basis for the kissing and nanotunneling, respectively. We propose that, through kissing and nanotunneling, the otherwise static mitochondria in a cardiomyocyte form one dynamically continuous network to share content and transfer signals.

  9. New upper bounds for kissing numbers from semidefinite programming

    NARCIS (Netherlands)

    C. Bachoc; F. Vallentin (Frank)

    2006-01-01

    htmlabstractRecently A. Schrijver derived new upper bounds for binary codes using semidefinite programming. In this paper we adapt this approach to codes on the unit sphere and we compute new upper bounds for the kissing number in several dimensions. In particular our computations give the (known)

  10. New upper bounds for kissing numbers from semidefinite programming

    NARCIS (Netherlands)

    C. Bachoc; F. Vallentin (Frank)

    2008-01-01

    htmlabstractRecently A. Schrijver derived new upper bounds for binary codes using semidefinite programming. In this paper we adapt this approach to codes on the unit sphere and we compute new upper bounds for the kissing number in several dimensions. In particular our computations give the (known)

  11. An improved design of the kissing complex-based aptasensor for the detection of adenosine.

    Science.gov (United States)

    Goux, Emma; Lisi, Samuele; Ravelet, Corinne; Durand, Guillaume; Fiore, Emmanuelle; Dausse, Eric; Toulmé, Jean-Jacques; Peyrin, Eric

    2015-08-01

    We very recently reported a novel aptamer biosensing concept based on a dual recognition mechanism originating from the small target-induced formation of a functional nucleic acid assembly. This assembly is constituted of a hairpin aptamer (named aptaswitch) for which the apical loop of the parent aptamer is substituted by a short RNA sequence prone to loop-loop interactions. It can switch between folded and unfolded states in the presence and in the absence of targets, respectively. The apical loop of the folded aptaswitch is then recognized by a second hairpin (called aptakiss), forming a kissing complex that signals the presence of the target. In the present work, we focus on the design improvement of this biosensing platform by using a previously described adenosine-adenoswitch couple as a model system and a fluorophore-labeled aptakiss as a reporting probe for fluorescence anisotropy (FA) detection. In the first step, the initially described adenoswitch was re-engineered to optimally convert the unfolded structure into the active stem-loop form upon adenosine binding. To further improve the assay performance, a blocking DNA oligonucleotide of the adenoswitch sequence was subsequently introduced into the assay scheme. This blocking strategy led to a significant increase in the FA response by reducing the background signal generated by the undesired binding of the free adenoswitch to the aptakiss probe. We obtained a detection limit which is fivefold lower than that observed with the previously reported kissing complex-based sensor. Finally, the optimized biosensing platform was successfully applied under biologically relevant conditions, i.e., diluted human serum, suggesting the potential practical applicability of the kissing sensing approach.

  12. Objects prompt authentic scientific activities among learners in a museum programme

    DEFF Research Database (Denmark)

    Achiam, Marianne; Simony, Leonora; Lindow, Bent Erik Kramer

    2016-01-01

    Although the scientific disciplines conduct practical work in different ways, all consider practical work as the essential way of connecting objects and phenomena with ideas and the abstract. Accordingly, practical work is regarded as central to science education as well. We investigate a practical......, object-based palaeontology programme at a natural history museum to identify how palaeontological objects prompt scientific activity among upper secondary school students. We first construct a theoretical framework based on an analysis of the programme’s palaeontological content. From this, we build our...... are palaeontologically authentic. Finally, we discuss our findings, examining the mechanism by which the specimens prompt scientific activities. We also discuss our discipline-based approach, and how it allows us to positively identify participants’ activities as authentic. We conclude by discussing the implications...

  13. The effect of ghrelin on Kiss-1 and KissR gene transcription and insulin secretion in rat islets of Langerhans and CRI-D2 cell line

    Directory of Open Access Journals (Sweden)

    Mandana Mahmoodzaeh Sagheb

    2017-01-01

    Full Text Available Objective(s: Ghrelin is a peptide hormone that has been shown to have numerous central and peripheral effects. The central effects including GH secretion, food intake, and energy homeostasis are partly mediated by Kiss1- KissR signaling pathway. Ghrelin and its receptor are also expressed in the pancreatic islets. Ghrelin is one of the key metabolic factors controlling insulin secretion from the islets of Langerhans. We hypothesize that the inhibitory effect of ghrelin on KiSS-1 and KissR in the islet cells may be similar to the same inhibitory effect of ghrelin in the hypothalamus. Materials and Methods: To investigate the effect of ghrelin, we isolated the islets from adult male rats by collagenase and cultured CRI-D2 cell lines. Then, we incubated them with different concentrations of ghrelin for 24 hr. After RNA extraction and cDNA synthesis from both islets and CRI-D2 cells, the relative expression of KiSS-1 and KissR was evaluated by means of real-time PCR. Furthermore, we measured the amount of insulin secreted by the islets after incubation in different concentrations of ghrelin and glucose after 1 hr. Besides, we checked the viability of the cells after 24 hr cultivation.  Results: Ghrelin significantly decreased the KiSS-1 and KissR mRNA transcription in rat islets and CRI-D2 cells. Besides, Ghrelin suppressed insulin secretion from pancreatic beta cells and CRI-D2 cells. Conclusion: These findings indicate the possibility that KiSS-1 and KissR mRNA expression is mediator of ghrelin function in the islets of Langerhans.

  14. The effect of ghrelin on Kiss-1 and KissR gene transcription and insulin secretion in rat islets of Langerhans and CRI-D2 cell line

    Science.gov (United States)

    Sagheb, Mandana Mahmoodzaeh; Azarpira, Negar; Mokhtary, Mokhtar

    2017-01-01

    Objective(s): Ghrelin is a peptide hormone that has been shown to have numerous central and peripheral effects. The central effects including GH secretion, food intake, and energy homeostasis are partly mediated by Kiss1- KissR signaling pathway. Ghrelin and its receptor are also expressed in the pancreatic islets. Ghrelin is one of the key metabolic factors controlling insulin secretion from the islets of Langerhans. We hypothesize that the inhibitory effect of ghrelin on KiSS-1 and KissR in the islet cells may be similar to the same inhibitory effect of ghrelin in the hypothalamus. Materials and Methods: To investigate the effect of ghrelin, we isolated the islets from adult male rats by collagenase and cultured CRI-D2 cell lines. Then, we incubated them with different concentrations of ghrelin for 24 hr. After RNA extraction and cDNA synthesis from both islets and CRI-D2 cells, the relative expression of KiSS-1 and KissR was evaluated by means of real-time PCR. Furthermore, we measured the amount of insulin secreted by the islets after incubation in different concentrations of ghrelin and glucose after 1 hr. Besides, we checked the viability of the cells after 24 hr cultivation. Results: Ghrelin significantly decreased the KiSS-1 and KissR mRNA transcription in rat islets and CRI-D2 cells. Besides, Ghrelin suppressed insulin secretion from pancreatic beta cells and CRI-D2 cells. Conclusion: These findings indicate the possibility that KiSS-1 and KissR mRNA expression is mediator of ghrelin function in the islets of Langerhans. PMID:28133522

  15. QCRI at TREC 2014: Applying the KISS Principle for the TTG Task in the Microblog Track

    Science.gov (United States)

    2014-11-01

    QCRI at TREC 2014: Applying the KISS principle for the TTG task in the Microblog Track Walid Magdy Qatar Computing Research Institute Qatar...straightforward ( KISS ) by using a simple implementation of Jaccard similarity to measure the distance between tweets in the top N retrieved results and...1. REPORT DATE NOV 2014 2. REPORT TYPE 3. DATES COVERED 00-00-2014 to 00-00-2014 4. TITLE AND SUBTITLE QCRI at TREC 2014: Applying the KISS

  16. Physical activity on prescription schemes (PARS): do programme characteristics influence effectiveness? Results of a systematic review and meta-analyses

    Science.gov (United States)

    Arsenijevic, Jelena; Groot, Wim

    2017-01-01

    Background Physical activity on prescription schemes (PARS) are health promotion programmes that have been implemented in various countries. The aim of this study was to outline the differences in the design of PARS in different countries. This study also explored the differences in the adherence rate to PARS and the self-reported level of physical activity between PARS users in different countries. Method A systematic literature review and meta-analyses were conducted. We searched PubMed and EBASCO in July 2015 and updated our search in September 2015. Studies that reported adherence to the programme and self-reported level of physical activity, published in the English language in a peer-reviewed journal since 2000, were included. The difference in the pooled adherence rate after finishing the PARS programme and the adherence rate before or during the PARS programme was 17% (95% CI 9% to 24%). The difference in the pooled physical activity was 0.93 unit score (95 CI −3.57 to 1.71). For the adherence rate, a meta-regression was conducted. Results In total, 37 studies conducted in 11 different countries met the inclusion criteria. Among them, 31 reported the adherence rate, while the level of physical activity was reported in 17 studies. Results from meta-analyses show that PARS had an effect on the adherence rate of physical activity, while the results from the meta-regressions show that programme characteristics such as type of chronic disease and the follow-up period influenced the adherence rate. Conclusions The effects of PARS on adherence and self-reported physical activity were influenced by programme characteristics and also by the design of the study. Future studies on the effectiveness of PARS should use a prospective longitudinal design and combine quantitative and qualitative data. Furthermore, future evaluation studies should distinguish between evaluating the adherence rate and the self-reported physical activity among participants with different

  17. Active Labour Market Programme Participation for Unemployment Insurance Recipients: A Systematic Review

    DEFF Research Database (Denmark)

    Filges, Trine; Smedslund, Geir; Knudsen, Anne-Sofie Due

    2015-01-01

    , subsequently, their employability. Other ALMPs are designed to encourage the unemployed to return to work. Typically, compulsory programme participation is required after the individual has received unemployment benefits for a certain period of time. A large variety of different ALMPs exist among countries....... They can consist of job search assistance, training, education, subsidized work and similar programmes. Some of the programmes (such as subsidized work, training and education) demand full-time participation over a long time period (e.g. several months), while other programmes (such as job search...... assistance and education) are part-time and have a short duration (e.g. few days/weeks). It is possible to classify these programmes into a set of four core categories: A: (labour market) training, B: Private sector programmes, C: direct employment programmes in the public sector and D: Job search assistance...

  18. Kissing molars and hyperplastic dental follicles: report of a case and literature review.

    Science.gov (United States)

    Kiran, Heggavadipura Yogendraswamy; Bharani, K S N Shiva; Kamath, Rajay Agnel; Manimangalath, Girisankar; Madhushankar, G S

    2014-01-01

    "Kissing" molars are impacted permanent molars that have occlusal surfaces contacting each other in a single follicular space, with roots pointing in opposite directions. It is deemed to be appropriate to medically investigate mucopolysaccharidosis (MPS) in patients presenting with kissing molars as kissing molars have been linked with MPS. The case of bilateral occurrence of kissing molars in an 18-year-old woman is described. Pathological analysis of the follicular tissue suggested hyperplastic dental follicles. Therefore, this case report analysed the association of impacted permanent teeth with hyperplastic dental follicles, following the review of seven documented reports describing such association.

  19. 2014 ICSA/KISS Joint Applied Statistics Symposium

    CERN Document Server

    Liu, Mengling; Luo, Xiaolong

    2016-01-01

    The papers in this volume represent the most timely and advanced contributions to the 2014 Joint Applied Statistics Symposium of the International Chinese Statistical Association (ICSA) and the Korean International Statistical Society (KISS), held in Portland, Oregon. The contributions cover new developments in statistical modeling and clinical research: including model development, model checking, and innovative clinical trial design and analysis. Each paper was peer-reviewed by at least two referees and also by an editor. The conference was attended by over 400 participants from academia, industry, and government agencies around the world, including from North America, Asia, and Europe. It offered 3 keynote speeches, 7 short courses, 76 parallel scientific sessions, student paper sessions, and social events. The most timely and advanced contributions from the joint 2014 ICSA/KISS Applied Statistics Symposium All papers feature original, peer-reviewed content Coverage consists of new developments in statisti...

  20. Conjectures regarding kissing spheres hierarchy and quantum gravity unification

    Energy Technology Data Exchange (ETDEWEB)

    El Naschie, M.S. [Department of Astrophysics, University of Cairo (Egypt); Donghua University Shanghai (China)], E-mail: Chaossf@aol.com

    2008-01-15

    Conjectures regarding connections between hyper-spheres kissing problems in one- to eight-dimensional spaces and the inverse coupling constant of quantum gravity are presented. In particular we demonstrate that in S{sup (7)} and S{sup (8)} the coupling constants of the non-super-symmetric and the super-symmetric unification are given by {alpha}-bar{sub g}={sup 7}{radical}(K(128)){approx_equal}42 and {alpha}-bar{sub gs}={sup 8}{radical}(K(128)){approx_equal}26 respectively where K(128) {approx_equal} (2.18)(10){sup 11} is the sphere kissing number in D = 128 Euclidian space.

  1. Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9.

    Science.gov (United States)

    Koo, Taeyoung; Lee, Jungjoon; Kim, Jin-Soo

    2015-06-01

    Programmable nucleases, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs) repurposed from the type II clustered, regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system are now widely used for genome editing in higher eukaryotic cells and whole organisms, revolutionising almost every discipline in biological research, medicine, and biotechnology. All of these nucleases, however, induce off-target mutations at sites homologous in sequence with on-target sites, limiting their utility in many applications including gene or cell therapy. In this review, we compare methods for detecting nuclease off-target mutations. We also review methods for profiling genome-wide off-target effects and discuss how to reduce or avoid off-target mutations.

  2. Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins.

    Science.gov (United States)

    Maeder, Morgan L; Angstman, James F; Richardson, Marcy E; Linder, Samantha J; Cascio, Vincent M; Tsai, Shengdar Q; Ho, Quan H; Sander, Jeffry D; Reyon, Deepak; Bernstein, Bradley E; Costello, Joseph F; Wilkinson, Miles F; Joung, J Keith

    2013-12-01

    Genome-wide studies have defined cell type-specific patterns of DNA methylation that are important for regulating gene expression in both normal development and disease. However, determining the functional significance of specific methylation events remains challenging, owing to the lack of methods for removing such modifications in a targeted manner. Here we describe an approach for efficient targeted demethylation of specific CpGs in human cells using fusions of engineered transcription activator-like effector (TALE) repeat arrays and the TET1 hydroxylase catalytic domain. Using these TALE-TET1 fusions, we demonstrate that modification of critical methylated promoter CpG positions can lead to substantial increases in the expression of endogenous human genes. Our results delineate a strategy for understanding the functional significance of specific CpG methylation marks in the context of endogenous gene loci and validate programmable DNA demethylation reagents with potential utility for research and therapeutic applications.

  3. The SEDIBUD (Sediment Budgets in Cold Environments) Programme: Current activities and future key tasks

    Science.gov (United States)

    Beylich, A. A.; Lamoureux, S. F.; Decaulne, A.

    2012-04-01

    Projected climate change in cold regions is expected to alter melt season duration and intensity, along with the number of extreme rainfall events, total annual precipitation and the balance between snowfall and rainfall. Similarly, changes to the thermal balance are expected to reduce the extent of permafrost and seasonal ground frost and increase active layer depths. These effects will undoubtedly change surface environments in cold regions and alter the fluxes of sediments, nutrients and solutes, but the absence of quantitative data and coordinated process monitoring and analysis to understand the sensitivity of the Earth surface environment is acute in cold climate environments. The International Association of Geomorphologists (I.A.G./A.I.G.)SEDIBUD (Sediment Budgets in Cold Environments) Programme was formed in 2005 to address this existing key knowledge gap. SEDIBUD currently has about 400 members worldwide and the Steering Committee of this international programme is composed of ten scientists from eight different countries: Achim A. Beylich (Chair) (Norway), Armelle Decaulne (Secretary) (France), John C. Dixon (USA), Scott F. Lamoureux (Vice-Chair) (Canada), John F. Orwin (Canada), Jan-Christoph Otto (Austria), Irina Overeem (USA), Thorsteinn Saemundsson (Iceland), Jeff Warburton (UK), Zbigniew Zwolinski (Poland). The central research question of this global group of scientists is to: Assess and model the contemporary sedimentary fluxes in cold climates, with emphasis on both particulate and dissolved components. Initially formed as European Science Foundation (ESF) Network SEDIFLUX (2004-2006), SEDIBUD has further expanded to a global group of researchers with field research sites located in polar and alpine regions in the northern and southern hemisphere. Research carried out at each of the close to 50 defined SEDIBUD key test sites varies by programme, logistics and available resources, but typically represent interdisciplinary collaborations of

  4. Heuristic RNA pseudoknot prediction including intramolecular kissing hairpins

    Science.gov (United States)

    Sperschneider, Jana; Datta, Amitava; Wise, Michael J.

    2011-01-01

    Pseudoknots are an essential feature of RNA tertiary structures. Simple H-type pseudoknots have been studied extensively in terms of biological functions, computational prediction, and energy models. Intramolecular kissing hairpins are a more complex and biologically important type of pseudoknot in which two hairpin loops form base pairs. They are hard to predict using free energy minimization due to high computational requirements. Heuristic methods that allow arbitrary pseudoknots strongly depend on the quality of energy parameters, which are not yet available for complex pseudoknots. We present an extension of the heuristic pseudoknot prediction algorithm DotKnot, which covers H-type pseudoknots and intramolecular kissing hairpins. Our framework allows for easy integration of advanced H-type pseudoknot energy models. For a test set of RNA sequences containing kissing hairpins and other types of pseudoknot structures, DotKnot outperforms competing methods from the literature. DotKnot is available as a web server under http://dotknot.csse.uwa.edu.au. PMID:21098139

  5. A primary school active break programme (ACTI-BREAK): study protocol for a pilot cluster randomised controlled trial.

    Science.gov (United States)

    Watson, Amanda; Timperio, Anna; Brown, Helen; Hesketh, Kylie D

    2017-09-19

    Levels of overall physical activity have been shown to decline across childhood. Schools are considered ideal settings to promote physical activity as children spend a large amount of their waking hours at school. Time-efficient physical activity strategies that demonstrate a positive impact on academic-related outcomes are needed to enable physical activity to be prioritised in the school day. The ACTI-BREAK programme requires classroom teachers to integrate active breaks; 5-min bursts of moderate-intensity physical activity into their classroom routine. Active breaks have been shown to be effective in improving academic-related outcomes, a potentially appealing aspect for teachers and schools. The primary aim of this study is to assess the feasibility and potential efficacy of the ACTI-BREAK programme on children's academic achievement. Secondary aims are to explore the impact of ACTI-BREAK on children's on-task behaviour and objectively measured physical activity levels. ACTI-BREAK is a 6-week, classroom-based, physical activity intervention. This pilot trial of the programme will be evaluated using a cluster randomised controlled design. Government primary schools in metropolitan Melbourne, Australia will be invited to participate in the programme in 2017. Randomisation will occur at the school level, with the aim to recruit six schools (three intervention and three control). The ACTI-BREAK programme is theoretically grounded, and was developed with input and guidance from current primary school teachers. Teachers from the intervention schools will receive a 45-min training session and be asked to incorporate ACTI-BREAKS into their classroom routine three times per day for 6 weeks. Intervention support will be provided via assisted delivery. The primary outcomes will be children's academic achievement in mathematics and reading. Children's on-task behaviour and school-day physical activity will be assessed as secondary outcomes. Process evaluation will also be

  6. Relationship between The Kiss-1 Gene Promoter Methylation and Kiss-1 Gene Expression of Colorectal Carcinoma%结直肠癌中Kiss-1基因启动子甲基化与Kiss-1基因表达的关系

    Institute of Scientific and Technical Information of China (English)

    林素勇; 陈志华; 陈绍勤; 戴起宝

    2013-01-01

    目的:研究结直肠癌中Kiss-1基因启动子甲基化状态对Kiss-1基因表达的影响。方法:应用甲基化特异性PCR (MSP)方法检测73例结直肠癌、正常结直肠组织和人结直肠癌细胞HCT116、SW480、W1116、LoVo中Kiss-1基因启动子甲基化状态,应用realtime-PCR、Western-blot技术检测相应组织和细胞中Kiss-1基因mRNA和蛋白质(Metastine)的表达量。结果:结直肠癌中Kiss-1基因甲基化阳性率(82.19%)高于正常组织(6.31%)(PSW480>SW1116>HCT116,差异有统计学意义(p<0.05)。结论:结直肠癌中Kiss-1基因启动子甲基化可能引起Kiss-1基因表达下调。%Objective:To research the effect of the Kiss-1 gene promoter methylation on Kiss-1 gene expression in colorectal carci-noma. Methods:Kiss-1 gene promotor methylation and its expression were detected respectively by methylation-specific PCR, real-time PCR and western-blot in 73 cases of colorectal carcinoma and each normal tissue. Results:The positive expression rate of Kiss-1 gene methylation in the carcinoma tissue was higher than the rate in normal tissue (82.19%vs 6.31%, P <0.05). In the cancer tissue, the Kiss-1 gene mRNA and metastine expression of Kiss-1 methylation-positive group was significant lower than that in negative group (P<0.05). Conclusions:Kiss-1 gene promoter hyper-methylation may induce to the Kiss-1 gene expression decreased in colorectal carci-noma.

  7. A multichannel integrated circuit for electrical recording of neural activity, with independent channel programmability.

    Science.gov (United States)

    Mora Lopez, Carolina; Prodanov, Dimiter; Braeken, Dries; Gligorijevic, Ivan; Eberle, Wolfgang; Bartic, Carmen; Puers, Robert; Gielen, Georges

    2012-04-01

    Since a few decades, micro-fabricated neural probes are being used, together with microelectronic interfaces, to get more insight in the activity of neuronal networks. The need for higher temporal and spatial recording resolutions imposes new challenges on the design of integrated neural interfaces with respect to power consumption, data handling and versatility. In this paper, we present an integrated acquisition system for in vitro and in vivo recording of neural activity. The ASIC consists of 16 low-noise, fully-differential input channels with independent programmability of its amplification (from 100 to 6000 V/V) and filtering (1-6000 Hz range) capabilities. Each channel is AC-coupled and implements a fourth-order band-pass filter in order to steeply attenuate out-of-band noise and DC input offsets. The system achieves an input-referred noise density of 37 nV/√Hz, a NEF of 5.1, a CMRR > 60 dB, a THD < 1% and a sampling rate of 30 kS/s per channel, while consuming a maximum of 70 μA per channel from a single 3.3 V. The ASIC was implemented in a 0.35 μm CMOS technology and has a total area of 5.6 × 4.5 mm². The recording system was successfully validated in in vitro and in vivo experiments, achieving simultaneous multichannel recordings of cell activity with satisfactory signal-to-noise ratios.

  8. Indian Solar Cities Programme: An Overview of Major Activities and Accomplishments; Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Kandt, A.

    2012-05-01

    This paper details the Indian Solar City Programme, provides an overview of one city's Master Plan and implementation progress, describes NREL's support of the Indian Solar City Programme, and outlines synergies and differences between the Indian and American programs including unique challenges and opportunities India is facing.

  9. Decrease in heart rate after longitudinal participation in the Groningen Active Living Model (GALM) recreational sports programme

    NARCIS (Netherlands)

    de Jong, Johan; Lemmink, Koen; Scherder, Erik; Stewart, Roy; King, Abby; Stevens, Martin

    2009-01-01

    The aim of this study was to investigate changes in heart rate during submaximal exercise as an index of cardiovascular function in older adults participating in the Groningen Active Living Model recreational sports programme who were sedentary or underactive at baseline. A repeated measurement desi

  10. Short- and long-term effects of a physical activity counselling programme in COPD : A randomized controlled trial

    NARCIS (Netherlands)

    Altenburg, Wytske A.; ten Hacken, Nick H. T.; Bossenbroek, Linda; Kerstjens, Huib A. M.; de Greef, Mathieu H.G.; Wempe, Johan B.

    2015-01-01

    Background: We were interested in the effects of a physical activity (PA) counselling programme in three groups of COPD patients from general practice (primary care), outpatient clinic (secondary care) and pulmonary rehabilitation (PR). Methods: In this randomized controlled trial 155 COPD patients,

  11. Decrease in heart rate after longitudinal participation in the Groningen Active Living Model (GALM) recreational sports programme

    NARCIS (Netherlands)

    de Jong, Johan; Lemmink, Koen; Scherder, Erik; Stewart, Roy; King, Abby; Stevens, Martin

    2009-01-01

    The aim of this study was to investigate changes in heart rate during submaximal exercise as an index of cardiovascular function in older adults participating in the Groningen Active Living Model recreational sports programme who were sedentary or underactive at baseline. A repeated measurement

  12. Longitudinal changes in heart rate after participating in the Groningen Active Living Model (GALM) recreational sports programme

    NARCIS (Netherlands)

    Erik Scherder; Abby King; Roy Stewart; Dr. Johan de Jong; Koen A.P.M. Lemmink

    2009-01-01

    The aim of this study was to investigate changes in heart rate during submaximal exercise as an index of cardiovascular function in older adults participating in the Groningen Active Living Model recreational sports programme who were sedentary or underactive at baseline. A repeated measurement

  13. Sexual activity as a trigger of myocardial infarction. A case-crossover analysis in the Stockholm Heart Epidemiology Programme (SHEEP)

    DEFF Research Database (Denmark)

    Möller, J; Ahlbom, A; Hulting, J

    2001-01-01

    OBJECTIVE: To investigate sexual activity as a trigger of myocardial infarction and the potential effect modification of physical fitness. DESIGN: A case-crossover study nested in the Stockholm Heart Epidemiology Programme (SHEEP). SETTING: Stockholm County from April 1993 to December 1994...

  14. From Passive to Active Learners: The "Lived Experience" of Nurses in a Specialist Nephrology Nursing Education Programme

    Science.gov (United States)

    Bridger, Jane

    2007-01-01

    Purpose: This study aims to explore the lived experience of learning for a group of staff nurses in the Middle East, who undertook a post-registration nursing education programme in the speciality of nephrology nursing (the NNP) between 2001 and 2002. The broad-based curriculum seeks to develop the staff nurses into active learners, able to…

  15. Endovascular Treatment of Occlusive Lesions in the Aortic Bifurcation with Kissing Polytetrafluoroethylene-Covered Stents

    NARCIS (Netherlands)

    Grimme, Frederike A. B.; Spithoven, J. Hans; Zeebregts, Clark J.; Scharn, Dirk M.; Reijnen, Michel M. P. J.

    Purpose: To determine the clinical outcomes of polytetrafluoroethylene covered balloon expandable stents (CBESs) in occlusive lesions of the aortic bifurcation in a kissing stent configuration. Materials and Methods: The study included 69 consecutive patients (29 men, 40 women) who underwent kissing

  16. Thermodynamic and kinetic analysis of an RNA kissing interaction and its resolution into an extended duplex.

    Science.gov (United States)

    Salim, Nilshad; Lamichhane, Rajan; Zhao, Rui; Banerjee, Tuhina; Philip, Jane; Rueda, David; Feig, Andrew L

    2012-03-07

    Kissing hairpin interactions form when the loop residues of two hairpins have Watson-Crick complementarity. In a unimolecular context, kissing interactions are important for tertiary folding and pseudoknot formation, whereas in a bimolecular context, they provide a basis for molecular recognition. In some cases, kissing complexes can be a prelude to strand displacement reactions where the two hairpins resolve to form a stable extended intermolecular duplex. The kinetics and thermodynamics of kissing-complex formation and their subsequent strand-displacement reactions are poorly understood. Here, biophysical techniques including isothermal titration calorimetry, surface plasmon resonance, and single-molecule fluorescence have been employed to probe the factors that govern the stability of kissing complexes and their subsequent structural rearrangements. We show that the general understanding of RNA duplex formation can be extended to kissing complexes but that kissing complexes display an unusual level of stability relative to simple duplexes of the same sequence. These interactions form and break many times at room temperature before becoming committed to a slow, irreversible forward transition to the strand-displaced form. Furthermore, using smFRET we show that the primary difference between stable and labile kissing complexes is based almost completely on their off rates. Both stable and labile complexes form at the same rate within error, but less stable species dissociate rapidly, allowing us to understand how these complexes can help generate specificity along a folding pathway or during a gene regulation event. Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  17. Endovascular Treatment of Occlusive Lesions in the Aortic Bifurcation with Kissing Polytetrafluoroethylene-Covered Stents

    NARCIS (Netherlands)

    Grimme, F.A.B.; Spithoven, J.H.; Zeebregts, C.J.A.; Scharn, D.M.; Reijnen, M.M.

    2015-01-01

    PURPOSE: To determine the clinical outcomes of polytetrafluoroethylene covered balloon expandable stents (CBESs) in occlusive lesions of the aortic bifurcation in a kissing stent configuration. MATERIALS AND METHODS: The study included 69 consecutive patients (29 men, 40 women) who underwent kissing

  18. Kissing Bug (Triatoma spp.) Intrusion into Homes: Troublesome Bites and Domiciliation.

    Science.gov (United States)

    Klotz, Stephen A; Shirazi, F Mazda; Boesen, Keith; Beatty, Norman L; Dorn, Patricia L; Smith, Shannon; Schmidt, Justin O

    2016-01-01

    Kissing bugs (Triatoma spp.) frequently enter homes and bite human and pet occupants. Bites may lead to severe allergic reactions and, in some cases, death. Kissing bugs are also vectors of Trypanosoma cruzi, the cause of Chagas disease. In general, modern houses in the United States are not conducive to domiciliation of kissing bugs (bugs living out their entire life within the home with the presence of eggs, nymphs, adults, and exuviae). Construction features such as concrete foundations, solid walls and ceilings, window screens, tight thresholds for doors and windows, and other measures impede bug entry into homes, and air conditioning reduces the need for open doors and windows. Where Chagas disease is endemic in Mexico and Central and South America, homes often have thatch roofs, adobe walls, and open doors and windows. We investigated numerous instances of kissing bug intrusions into homes in Southern Arizona, California, and Louisiana and documented the reactions to kissing bug bites. Our work confirms the importance of modern home construction in limiting kissing bug intrusions. Older homes, especially those lacking modern screening, caulking, and weather stripping to reduce air leakage, may be subject to kissing bug intrusions and domiciliation. We describe a community in Southern Arizona where domiciliation of homes by Triatoma recurva is common. We also provide recent data regarding kissing bug bites and allergic reactions to the bites.

  19. Care of older people in nursing homes: an Intensive Programme as an educational activity within Erasmus-Socrates.

    Science.gov (United States)

    Kotzabassaki, Stella; Alabaster, Erica S; And, Kati; Larsson, Ulla; de Vree, Willem

    2003-02-01

    The paper describes and discusses an Intensive Programme as a European educational activity within Erasmus-Socrates. As nurses and European citizens, it is important to know and understand each other's culture and to be able to work within a united Europe. Education plays a leading role in the preparation of professionals who will have to develop these skills. Based on the aims of Erasmus-Socrates, an Intensive Programme entitled 'Care of Older People in Nursing Homes' was designed, sponsored, and implemented over three continuous academic years with the participation of five European countries. The topic was selected due to its importance for Europe, as it is a region with an ageing population. A wide range of themes was covered using lectures, group discussion, exercises and study visits as teaching strategies. Evaluation suggests that the aims of the programme were achieved.

  20. Clinical Significance of KISS1 Protein Expression for Brain Invasion and Metastasis

    Science.gov (United States)

    Ulasov, Ilya V.; Kaverina, Natalya V.; Pytel, Peter; Thaci, Bart; Liu, FeiFei; Hurst, Douglas R.; Welch, Danny R.; Sattar, Husein A.; Olopade, Olufunmilayo I.; Baryshnikov, Anatoly Y.; Kadagidze, Zaira G.; Lesniak, Maciej S.

    2013-01-01

    BACKGROUND Metastases to the brain represent a feared complication and contribute to the morbidity and mortality of breast cancer. Despite improvements in therapy, prognostic factors for development of metastases are lacking. KISS1 is a metastasis suppressor that demonstrates inhibition of metastases formation in several types of cancer. The purpose of this study was to determine the importance of KISS1 expression in breast cancer progression and the development of intracerebral lesions. METHODS In this study, we performed a comparative analysis of 47 brain metastases and 165 primary breast cancer specimens by using the antihuman KISS1 antibody. To compare KISS1 expression between different groups, we used a 3-tier score and the automated score computer software (ACIS) evaluation. To reveal association between mRNA and protein expression, we used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. Significance of immunohistochemistry stainings was correlated with clinicopathological data. RESULTS We identified that KISS1 expression is significantly higher in primary breast cancer compared with brain metastases (P < .05). The mRNA analysis performed on 33 selected ductal carcinoma brain metastatic lesions and 36 primary ductal carcinomas revealed a statistically significant down-regulation of KISS1 protein in metastatic cases (P = .04). Finally, we observed a significant correlation between expression of KISS1 and metastasis-free survival (P = .04) along with progression of breast cancer and expression of KISS1 in primary breast cancer specimens (P = .044). CONCLUSIONS In conclusion, our study shows that breast cancer expresses KISS1. Cytoplasmic expression of KISS1 may be used as a prognostic marker for increased risk of breast cancer progression. PMID:21928364

  1. Mechanical unfolding of two DIS RNA kissing complexes from HIV-1.

    Science.gov (United States)

    Li, Pan T X; Tinoco, Ignacio

    2009-03-13

    An RNA kissing complex formed by the dimerization initiation site plays a critical role in the survival and infectivity of human immunodeficiency virus. Two dimerization initiation site kissing sequences, Mal and Lai, have been found in most human immunodeficiency virus 1 variants. Formation and stability of these RNA kissing complexes depend crucially on cationic conditions, particularly Mg 2+. Using optical tweezers, we investigated the mechanical unfolding of single RNA molecules with either Mal-type (GUGCAC) or Lai-type (GCGCGC) kissing complexes under various ionic conditions. The force required to disrupt the kissing interaction of the two structures, the rip force, is sensitive to concentrations of KCl and MgCl2; addition of 3 mM MgCl2 to 100 mM KCl changes the rip force of Mal from 21 +/- 4 to 46 +/- 3 pN. From the rip force distribution, the kinetics of breaking the kissing interaction is calculated as a function of force and cation concentration. The two kissing complexes have distinct unfolding transition states, as shown by different values of deltaX(++), which is the distance from the folded structure to the unfolding transition state. The deltaX(++) of Mal is approximately 0.6 nm smaller than that of Lai, suggesting that fewer kissing base pairs are broken at the transition state of the former, consistent with observations that the Lai-type kissing complex is more stable and requires significantly more force to unfold than the Mal type. More importantly, neither K+ nor Mg 2+ significantly changes the position of the transition state along the reaction coordinate. However, increasing concentrations of cations increase the kinetic barrier. We derived a cation-specific parameter, m, to describe how the height of the kinetic barrier depends on the concentration of cations. Our results suggest that Mg 2+ greatly slows down the unfolding of the kissing complex but has moderate effects on the formation kinetics of the structure.

  2. A Structured Physical Activity and Fitness Programme for Older Adults with Intellectual Disabilities: Results of a Cluster-Randomised Clinical Trial

    Science.gov (United States)

    van Schijndel-Speet, M.; Evenhuis, H. M.; van Wijck, R.; van Montfort, K. C. A. G. M.; Echteld, M. A.

    2017-01-01

    Background: The physical activity level of older adults with intellectual disabilities (ID) is extremely low, and their fitness levels are far beneath accepted norms for older people with normal intelligence and comparable with frail older people. A physical activity programme, including an education programme, was developed for older adults with…

  3. A Structured Physical Activity and Fitness Programme for Older Adults with Intellectual Disabilities: Results of a Cluster-Randomised Clinical Trial

    Science.gov (United States)

    van Schijndel-Speet, M.; Evenhuis, H. M.; van Wijck, R.; van Montfort, K. C. A. G. M.; Echteld, M. A.

    2017-01-01

    Background: The physical activity level of older adults with intellectual disabilities (ID) is extremely low, and their fitness levels are far beneath accepted norms for older people with normal intelligence and comparable with frail older people. A physical activity programme, including an education programme, was developed for older adults with…

  4. Technology Programme

    Energy Technology Data Exchange (ETDEWEB)

    Batistoni, Paola; De Marco, Francesco; Pieroni, Leonardo (ed.)

    2005-07-01

    The technology activities carried out by the Euratom-ENEA Association in the framework of the European Fusion Development Agreement concern the Next Step (International Thermonuclear Experimental Reactor - ITER), the Long-Term Programme (breeder blanket, materials, International Fusion Materials Irradiation Facility - IFMIF), Power Plant Conceptual Studies and Socio-Economic Studies. The Underlying Technology Programme was set up to complement the fusion activities as well to develop technologies with a wider range of interest. The Technology Programme mainly involves staff from the Frascati laboratories of the Fusion Technical and Scientific Unit and from the Brasimone laboratories of the Advanced Physics Technologies Unit. Other ENEA units also provide valuable contributions to the programme. ENEA is heavily engaged in component development/testing and in design and safety activities for the European Fusion Technology Programme. Although the work documented in the following covers a large range of topics that differ considerably because they concern the development of extremely complex systems, the high level of integration and coordination ensures the capability to cover the fusion system as a whole. In 2004 the most significant testing activities concerned the ITER primary beryllium-coated first wall. In the field of high-heat-flux components, an important achievement was the qualification of the process for depositing a copper liner on carbon fibre composite (CFC) hollow tiles. This new process, pre-brazed casting (PBC), allows the hot radial pressing (HRP) joining procedure to be used also for CFC-based armour monoblock divertor components. The PBC and HRP processes are candidates for the construction of the ITER divertor. In the materials field an important milestone was the commissioning of a new facility for chemical vapour infiltration/deposition, used for optimising silicon carbide composite (SiCf/SiC) components. Eight patents were deposited during 2004

  5. A programmable broadband low frequency active vibration isolation system for atom interferometry

    Science.gov (United States)

    Tang, Biao; Zhou, Lin; Xiong, Zongyuan; Wang, Jin; Zhan, Mingsheng

    2014-09-01

    Vibration isolation at low frequency is important for some precision measurement experiments that use atom interferometry. To decrease the vibrational noise caused by the reflecting mirror of Raman beams in atom interferometry, we designed and demonstrated a compact stable active low frequency vibration isolation system. In this system, a digital control subsystem is used to process and feedback the vibration measured by a seismometer. A voice coil actuator is used to control and cancel the motion of a commercial passive vibration isolation platform. With the help of field programmable gate array-based control subsystem, the vibration isolation system performed flexibly and accurately. When the feedback is on, the intrinsic resonance frequency of the system will change from 0.8 Hz to about 0.015 Hz. The vertical vibration (0.01-10 Hz) measured by the in-loop seismometer is reduced by an additional factor of up to 500 on the basis of a passive vibration isolation platform, and we have proved the performance by adding an additional seismometer as well as applying it in the atom interferometry experiment.

  6. A programmable broadband low frequency active vibration isolation system for atom interferometry.

    Science.gov (United States)

    Tang, Biao; Zhou, Lin; Xiong, Zongyuan; Wang, Jin; Zhan, Mingsheng

    2014-09-01

    Vibration isolation at low frequency is important for some precision measurement experiments that use atom interferometry. To decrease the vibrational noise caused by the reflecting mirror of Raman beams in atom interferometry, we designed and demonstrated a compact stable active low frequency vibration isolation system. In this system, a digital control subsystem is used to process and feedback the vibration measured by a seismometer. A voice coil actuator is used to control and cancel the motion of a commercial passive vibration isolation platform. With the help of field programmable gate array-based control subsystem, the vibration isolation system performed flexibly and accurately. When the feedback is on, the intrinsic resonance frequency of the system will change from 0.8 Hz to about 0.015 Hz. The vertical vibration (0.01-10 Hz) measured by the in-loop seismometer is reduced by an additional factor of up to 500 on the basis of a passive vibration isolation platform, and we have proved the performance by adding an additional seismometer as well as applying it in the atom interferometry experiment.

  7. Long Term Results of Kissing Stents in the Aortic Bifurcation.

    Science.gov (United States)

    Hinnen, J W; Konickx, M A; Meerwaldt, R; Kolkert, J L P; van der Palen, J; Huisman, A B; Geelkerken, R H

    2015-01-01

    To evaluate the long-term outcome after aortoiliac kissing stent placement and to analyze variables, which potentially influence the outcome of endovascular reconstruction of the aortic bifurcation. All patients treated with aortoiliac kissing stents at our institution between April 1995 and August 2011 were retrospectively identified from a prospective single-center database. Data regarding patient characteristics (age, gender, smoking, cardio- and cerebrovascular risk factors, hyperlipidaemia, diabetes mellitus and use of antihypertensive medication), symptoms, pre-interventional examination and imaging, procedural details and follow-up were retrieved. Patency rates were calculated with Kaplan-Meier analysis. Factors affecting the patency were determined with Cox uni- and multivariate analysis. A total of 215 patients (63% men, mean age 61 ± 10 years) were included. The median follow-up period was 31 (IQR 47.1) months. Primary, primary assisted, and secondary patency rates were 97%, 97%, and 99%, respectively, at one month; 92%, 95% and 94% at four months; 75%, 86%, and 91% at two years; 70%, 81%, and 91% at 5 years; and 67%, 81%, and 91% at ten years. Younger age and previous aortoiliac treatment were predictors for reduced primary and primary assisted patency. Smoking, previous aortoiliac intervention, TASC C and D lesions were predictors for reduced secondary patency. Reconstruction of the aortoiliac bifurcation with kissing stents is feasible, safe and effective in all types of lesions with satisfying long term patencies. TASC C and D lesions are associated with a higher occlusion rate. Younger age and previous aortoiliac interventions are predictors for reduced primary and primary assisted patency. © Acta Chirurgica Belgica.

  8. Kiss my asterisk a feisty guide to punctuation and grammar

    CERN Document Server

    Baranick, Jenny

    2014-01-01

    Grammar has finally let its hair down! Unlike uptight grammar books that overwhelm us with every single grammar rule, Kiss My Asterisk is like a bikini: it's fun, flirty, and covers only the most important bits. Its lessons, which are 100 percent free of complicated grammar jargon, have been carefully selected to include today's most common, noticeable errors—the ones that confuse our readers or make them wonder if we are, in fact, smarter than a fifth grader. What is the proper use of an apostrophe? When should an ellipsis be used instead of an em dash? Why do we capitalize President Obama bu

  9. Structure and stability of RNA/RNA kissing complex: with application to HIV dimerization initiation signal.

    Science.gov (United States)

    Cao, Song; Chen, Shi-Jie

    2011-12-01

    We develop a statistical mechanical model to predict the structure and folding stability of the RNA/RNA kissing-loop complex. One of the key ingredients of the theory is the conformational entropy for the RNA/RNA kissing complex. We employ the recently developed virtual bond-based RNA folding model (Vfold model) to evaluate the entropy parameters for the different types of kissing loops. A benchmark test against experiments suggests that the entropy calculation is reliable. As an application of the model, we apply the model to investigate the structure and folding thermodynamics for the kissing complex of the HIV-1 dimerization initiation signal. With the physics-based energetic parameters, we compute the free energy landscape for the HIV-1 dimer. From the energy landscape, we identify two minimal free energy structures, which correspond to the kissing-loop dimer and the extended-duplex dimer, respectively. The results support the two-step dimerization process for the HIV-1 replication cycle. Furthermore, based on the Vfold model and energy minimization, the theory can predict the native structure as well as the local minima in the free energy landscape. The root-mean-square deviations (RMSDs) for the predicted kissing-loop dimer and extended-duplex dimer are ~3.0 Å. The method developed here provides a new method to study the RNA/RNA kissing complex.

  10. The Impact of Adventure Based Activity at Malaysian National Service Training Programme on Team Cohesion: A Demographic Analysis.

    Directory of Open Access Journals (Sweden)

    Jaffry Zakaria

    2012-12-01

    Full Text Available The present study examines the effects of physical module elements (adventure based activity included in the Malaysian National Service Programme and to investigate the socio-demographic variables impact on team cohesion building among the participants. In this study, the participants were selected from three different camps, namely, Tasoh camp, Guar Chenderai camp and Meranti camp, located in the state of Perlis, Malaysia. The participants were those from the second batch intake in the year 2007. The sample sizes comprised of 994 participants (480=males, 514=females. The present study applied a pre-test and post-test design. The group was tested before and after the programme. This design was chosen because it could measure the impacts of the adventure-based activity towards team cohesion over time through the pre- and post-test scores (Berg & Latin, 2004. The variables comprised of the demographic factors (age, gender and place of residence, which included team cohesion were measured by Group Environmental Questionnaire (GEQ (Carron, Widmeyer, & Brawley, 1985. Results indicated that the participants of the National Service Training Programme underwent group cohesion change during the Physical Module activities (adventure based activities that were carried out at the camp. The changes were observable based on the statistically analyzed data during the pre and post-test.

  11. Scientific Goals of the Kunlun Infrared Sky Survey (KISS)

    CERN Document Server

    Burton, Michael; Mould, Jeremy; Cooke, Jeff; Ireland, Michael; Uddin, Syed Ashraf; Zhang, Hui; Yuan, Xiangyan; Lawrence, Jon; Ashley, Michael; Wu, Xuefend; Curtin, Chris; Wang, Lifan

    2016-01-01

    The high Antarctic plateau provides exceptional conditions for conducting infrared observations of the cosmos on account of the cold, dry and stable atmosphere above the ice surface. This paper describes the scientific goals behind the first program to examine the time-varying universe in the infrared from Antarctica - the Kunlun Infrared Sky Survey (KISS). This will employ a small (50 cm aperture) telescope to monitor the southern skies in the 2.4um Kdark window from China's Kunlun station at Dome A, on the summit of the Antarctic plateau, through the uninterrupted 4-month period of winter darkness. An earlier paper discussed optimisation of the Kdark filter for the best sensitivity (Li et al 2016). This paper examines the scientific program for KISS. We calculate the sensitivity of the camera for the extrema of observing conditions that will be encountered. We present the parameters for sample surveys that could then be carried out for a range of cadences and sensitivities. We then discuss several science p...

  12. Scientific Goals of the Kunlun Infrared Sky Survey (KISS)

    Science.gov (United States)

    Burton, Michael G.; Zheng, Jessica; Mould, Jeremy; Cooke, Jeff; Ireland, Michael; Uddin, Syed Ashraf; Zhang, Hui; Yuan, Xiangyan; Lawrence, Jon; Ashley, Michael C. B.; Wu, Xuefeng; Curtin, Chris; Wang, Lifan

    2016-09-01

    The high Antarctic plateau provides exceptional conditions for infrared observations on account of the cold, dry and stable atmosphere above the ice surface. This paper describes the scientific goals behind the first program to examine the time-varying universe in the infrared from Antarctica - the Kunlun Infrared Sky Survey (KISS). This will employ a 50cm telescope to monitor the southern skies in the 2.4μmK dark window from China's Kunlun station at Dome A, on the summit of the Antarctic plateau, through the uninterrupted 4-month period of winter darkness. An earlier paper discussed optimisation of the K dark filter for sensitivity (Li et al. 2016). This paper examines the scientific program for KISS. We calculate the sensitivity of the camera for the extrema of observing conditions that will be encountered. We present the parameters for sample surveys that could then be carried out for a range of cadences and sensitivities. We then discuss several science programs that could be conducted with these capabilities, involving star formation, brown dwarfs and hot Jupiters, exoplanets around M dwarfs, the terminal phases of stellar evolution, fast transients, embedded supernova searches, reverberation mapping of AGN, gamma ray bursts and the detection of the cosmic infrared background.

  13. Study of kissing molars in Turkish population sample.

    Science.gov (United States)

    Yanik, S; Ayranci, F; İşman, Ö; Büyükçikrikci, Ş; Aras, M H

    2017-06-01

    "Kissing" or "rosetting" of molars are extremely rare phenomenon with limited cases in the dental literature. It was first described in 1973, refers to contacting occlusal surfaces of the impacted mandibular second and third molars. The aim of the present study was to report the incidence of kissing molars (KMs), classification, incorporated pathologies, and its management in a group of Turkish population. The panoramic radiographs of the patients who referred to Gaziantep University Faculty of Dentistry between January 2012 and November 2014 for surgical treatment retrospectively were evaluated. The cases of KM were determined and evaluated with respect to its type, combined pathology, and treatment. Of the 6570 radiographs included in the study, 4 were seen to present as KM illustrating 0.060% of the sample. Three cases were Class II (0.045%), and only one case was encountered as Class III (0.015%). The mean age at the time that the KM teeth were identified was 34 years with a range from 29 to 40 years. Three of the patients were male, one of the patients was female, and all cases were seen unilaterally. One of the KMs was associated with dentigerous cyst formation. KM is a very rare clinical condition and few treatment options described. Early detection is essential to preclude complications and to provide more successful treatment. In this study, we evaluated the cases of KM and review of the literature also presented.

  14. Astrocytes promote progression of breast cancer metastases to the brain via a KISS1-mediated autophagy.

    Science.gov (United States)

    Kaverina, Natalya; Borovjagin, Anton V; Kadagidze, Zaira; Baryshnikov, Anatoly; Baryshnikova, Maria; Malin, Dmitry; Ghosh, Dhimankrishhna; Shah, Nameeta; Welch, Danny R; Gabikian, Patrik; Karseladze, Apollon; Cobbs, Charles; Ulasov, Ilya V

    2017-10-05

    Formation of metastases, also known as cancer dissemination, is an important stage of breast cancer (BrCa) development. KISS1 expression is associated with inhibition of metastases development. Recently we have demonstrated that BrCa metastases to the brain exhibit low levels of KISS1 expression at both mRNA and protein levels. By using multicolor immunofluorescence and coculture techniques here we show that normal adult astrocytes in the brain are capable of promoting metastatic transformation of circulating breast cancer cells localized to the brain through secretion of chemokine CXCL12. The latter was found in this study to downregulate KISS1 expression at the post-transcriptional level via induction of microRNA-345 (MIR345). Furthermore, we demonstrated that ectopic expression of KISS1 downregulates ATG5 and ATG7, 2 key modulators of autophagy, and works concurrently with autophagy inhibitors, thereby implicating autophagy in the mechanism of KISS1-mediated BrCa metastatic transformation. We also found that expression of KISS1 in human breast tumor specimens inversely correlates with that of MMP9 and IL8, implicated in the mechanism of metastatic invasion, thereby supporting the role of KISS1 as a potential regulator of BrCa metastatic invasion in the brain. This conclusion is further supported by the ability of KISS1, ectopically overexpressed from an adenoviral vector in MDA-MB-231Br cells with silenced expression of the endogenous gene, to revert invasive phenotype of those cells. Taken together, our results strongly suggest that human adult astrocytes can promote brain invasion of the brain-localized circulating breast cancer cells by upregulating autophagy signaling pathways via the CXCL12-MIR345- KISS1 axis.

  15. An Automated Software Package for the KISS Objective-Prism Survey for Emission-Line Galaxies. II. Recent Additions and Project Status

    Science.gov (United States)

    Frattare, L. M.; Salzer, J. J.

    1996-05-01

    We present an update on the KPNO International Spectroscopic Survey (KISS) project. KISS is a wide-field survey for extragalactic emission-line objects being carried out with the Burrell Schmidt at Kitt Peak. While we are utilizing the classical objective-prism technique to find strong-lined star-forming galaxies and AGNs, the use of CCD detectors and automated reduction software promise to make KISS a powerful tool for the study of activity in galaxies. We are currently completing our first survey strip (100 square degrees). The data consist of deep (to B = 20) objective-prism images, deep direct images in both B and V, and small-format photometric calibration images of each field. The KISS reduction package was designed to run under the IRAF image processing environment, and will eventually grow to be a complete IRAF package. Tasks added to the package over the past year include precise astrometry and photometry modules. The astrometry routines utilize the HST Guide Star Catalog to perform a full plate solution on the direct image of each Schmidt field, and then assign accurate equatorial coordinates to each object in the field. The photometry module performs aperture photometry on the direct images for all objects in the KISS database catalog, and provides routines to transfer the photometry calibration from the small-format images taken under photometric conditions to the large-format survey images. Extensive tests and modifications have also been carried out on the pre-existing software described by Herrero & Salzer (1995) in order to better fine-tune the reduction procedures and parameter settings. In addition to presenting a complete description of the new software, we describe the current status of the survey and present some preliminary characteristics of the sample. Other members of the KISS project include V. Lipovetsky & A. Kniazev (S.A.O.), T. Boroson (NOAO/USGP), T. Thuan (U. Virginia), J. Moody (BYU), Y. Izotov (Ukrainian Acad. Sci.), and J. Herrero

  16. [Self-management programme for leg ulcer patients: increased physical activity and fewer wound days

    NARCIS (Netherlands)

    Heinen, M.M.; Borm, G.F.; Vleuten, C.J. van der; Evers, A.W.; Oostendorp, R.A.B.; Achterberg, T. van

    2012-01-01

    OBJECTIVE: To investigate the effectiveness of the Lively Legs programme on patient adherence to compression therapy, walking behaviour and leg exercising, as well as on leg ulcer recurrence. DESIGN: Randomized controlled trial (ClinicalTrials.gov identifier: NCT00184873). METHOD: A total of 184 pat

  17. Active surveillance and control programme for Salmonella Dublin in Cattle

    DEFF Research Database (Denmark)

    Nielsen, Liza Rosenbaum; Rattenborg, Erik

    2011-01-01

    -positive. This suggests that there might be synergistic benefits from running control programmes for both infections simultaneously. We did not find the risk of becoming test-positive significantly different between organic and conventional herds, nor between analysing laboratories. Even when controlling for other risk...

  18. The Daughter's Disenchantment: Incest as Pedagogy in Fairy Tales and Kathryn Harrison's the Kiss

    Science.gov (United States)

    Marshall, Elizabeth

    2004-01-01

    The Kiss, is described as a controversial memoir about father-daughter incest that disturbed the cultural silence in a "well heeled" home. The emotional and psychological terrain of the daughter's experience is discussed.

  19. Kissing bugs in the United States: risk for vector-borne disease in humans.

    Science.gov (United States)

    Klotz, Stephen A; Dorn, Patricia L; Mosbacher, Mark; Schmidt, Justin O

    2014-01-01

    Eleven species of kissing bugs are found in the United States. Their home ranges may be expanding northward, perhaps as a consequence of climate change. At least eight of the species, perhaps all, are reported to harbor Trypanosoma cruzi, the parasite that causes Chagas disease. Because humans are encroaching on kissing bug habitat, there is concern for vector-transmitted Chagas disease in the United States. To date, documented autochthonous cases of Chagas in humans in the United States are rare. Kissing bugs are capable of adapting to new habitats such as human domiciles; however, they do not colonize homes in the United States as in Central and South America. We review the biology, behavior, and medical importance of kissing bugs and the risk they pose for transmission of Chagas disease in the United States. Where possible, descriptions of US species are compared to the epidemiologically important Latin American species.

  20. Thyroid Hormone Upregulates Hypothalamic kiss2 Gene in the Male Nile Tilapia, Oreochromis niloticus

    Directory of Open Access Journals (Sweden)

    Satoshi eOgawa

    2013-11-01

    Full Text Available Kisspeptin has recently been recognized as a critical regulator of reproductive function in vertebrates. During the sexual development, kisspeptin neurons receive sex steroids feedback to trigger gonadotropin-releasing hormone (GnRH neurons. In teleosts, a positive correlation has been found between the thyroid status and the reproductive status. However, the role of thyroid hormone in the regulation of kisspeptin system remains unknown. We cloned and characterized a gene encoding kisspeptin (kiss2 in a cichlid fish, the Nile tilapia (Oreochromis niloticus. Expression of kiss2 mRNA in the brain was analyzed by in situ hybridization. The effect of thyroid hormone (triiodothyronine, T3 and hypothyroidism with methimazole (MMI on kiss2 and the three GnRH types (gnrh1, gnrh2 and gnrh3 mRNA expression was analyzed by real-time PCR. Expression of thyroid hormone receptor mRNAs were analyzed in laser-captured kisspeptin and GnRH neurons by RT-PCR. The kiss2 mRNA expressing cells were seen in the nucleus of the lateral recess in the hypothalamus. Intraperitoneal administration of T3 (5µg/g body weight to sexually mature male tilapia significantly increased kiss2 and gnrh1 mRNA levels at 24 hr post injection (P < 0.001, while the treatment with an anti-thyroid, MMI (100 ppm for 6 days significantly reduced kiss2 and gnrh1 mRNA levels (P < 0.05. gnrh2, gnrh3 and thyrotropin-releasing hormone mRNA levels were insensitive to the thyroid hormone manipulations. Furthermore, RT-PCR showed expression of thyroid hormone receptor mRNAs in laser-captured GnRH neurons but not in kiss2 neurons. This study shows that GnRH1 may be directly regulated through thyroid hormone, while the regulation of Kiss2 by T3 is more likely to be indirect.

  1. Kissing contusion between the posterolateral tibial plateau and lateral femoral condyle: associated ligament and meniscal tears

    Energy Technology Data Exchange (ETDEWEB)

    Hong, Hyun Pyo; Lee, Jae Gue; Park, Ji Seon; Ryu, Kyung Nam [Kyunghee Univ., Seoul (Korea, Republic of)

    2004-02-01

    Kissing contusion between the posterolateral tibial plateau and lateral femoral condyle is frequently found in association with a tear of the anterior cruciate liagment (ACL). The purpose of this study was to determine which ligamentous and meniscal tears are associated with kissing contusion. We retrospectively reviewed the findings depicted by 323 consecutive MR images of the knee and confirmed at arthroscopy. For the diagnosis of disruption, ligaments, medial menisci (MM) and lateral menisci (LM) were evaluated using accepted criteria. We compared the prevalence and location of meniscal and ligamentous tears between group I (44 knees with kissing contusion) and group II (279 knees without kissing contusion). For statistical analysis the chi-square test was used. ACLs were torn in all 44 knees (100%) with kissing contusion, and 78 (28%) of 279 without kissing contusion. There were ten medial collateral ligament (MCL) tears (23%) in group I, and 17 MCL tears (6%), five lateral collateral ligament (LCL) tears (2%) and ten posterior cruciate ligament (PCL) tears (4%) in group II. In group I, meniscal tears were found in 22 MM (50%) and in 19 LM (43%), while in group II, they occurred in 128 MM (46%) and 128 LM (46%), In group I, 17 (77%) of 22 MM tears and 13 (68%) of 19 LM tears were located in the posterior horn, while in group II, the corresponding figures were 97/128 (76%) and 60 of 128 (47%). The differing prevalence of ACL and MCL tears between the groups was statistically significant (p<0.05), but differences in the prevalence and location of meniscal tears were not (p>0.05). Although kissing contusion was a highly specific sign of ACL tears, its presence was also significant among MCL tears. There was no significant difference in meniscal tears with or without kissing contusion.

  2. THE FINANCING OF THE ACTIVITIES WITHIN THE SECTORIAL OPERATIONAL PROGRAMME “INCREASE OF ECONOMIC COMPETITIVENESS”

    OpenAIRE

    Vasile POPEANGĂ

    2009-01-01

    The Sectorial Operational Programme “Increase of Economic Competitiveness” (further referred to as SOP IEC) is one of the seven instruments (OPs), under the Convergence objective, for achieving the priorities of the National Strategic Reference Framework (NSRF) derived from the National Development Plan 2007 – 2013 (NDP), which aims to strengthen the strategic focus of the Economic and Social Cohesion policies across Romania, and to make the correct and appropriate ...

  3. Mechanism of regulation of 'chromosome kissing' induced by Fob1 and its physiological significance.

    Science.gov (United States)

    Choudhury, Malay; Zaman, Shamsu; Jiang, James C; Jazwinski, S Michal; Bastia, Deepak

    2015-06-01

    Protein-mediated "chromosome kissing" between two DNA sites in trans (or in cis) is known to facilitate three-dimensional control of gene expression and DNA replication. However, the mechanisms of regulation of the long-range interactions are unknown. Here, we show that the replication terminator protein Fob1 of Saccharomyces cerevisiae promoted chromosome kissing that initiated rDNA recombination and controlled the replicative life span (RLS). Oligomerization of Fob1 caused synaptic (kissing) interactions between pairs of terminator (Ter) sites that initiated recombination in rDNA. Fob1 oligomerization and Ter-Ter kissing were regulated by intramolecular inhibitory interactions between the C-terminal domain (C-Fob1) and the N-terminal domain (N-Fob1). Phosphomimetic substitutions of specific residues of C-Fob1 counteracted the inhibitory interaction. A mutation in either N-Fob1 that blocked Fob1 oligomerization or C-Fob1 that blocked its phosphorylation antagonized chromosome kissing and recombination and enhanced the RLS. The results provide novel insights into a mechanism of regulation of Fob1-mediated chromosome kissing. © 2015 Choudhury et al.; Published by Cold Spring Harbor Laboratory Press.

  4. Efficiency of the home parental programme in visual-motor home activity among first-grade children.

    Science.gov (United States)

    Ratzon, N Z; Zabaneh-Tannas, K; Ben-Hamo, L; Bart, Orit

    2010-03-01

    The objectives of this study were to assess the efficacy of the home parental programme (HPP) in improving children's visual-motor skills, and to assess parent satisfaction with the programme. Forty-five children and their parents participated in the study: 23 children and their parents in the study group (children receiving consultation in the classroom and whose parents participated in the HPP) and 22 children and their parents in the control group (children receiving consultation in the classroom only). All children were recruited based on their scores on the Visual Motor Integration test or upon teacher referral. The Developmental Test of Visual Perception-2 administered before and after intervention served as an outcome measure. HPP lasted approximately 12 weeks and included relevant visual-motor activities carried out at home by the parents with their children. The study failed to prove that HPP improved children's visual-motor skills beyond the other service delivery methods used in the present study. Parent satisfaction was significantly higher among those who participated in the HPP. Parents showed high compliance with the friendly programme despite their low socio-economic status.

  5. REVIEW OF THE NATIONAL RESTOCKING PROGRAMMEACTIVE PROTECTION OF NATIVE CRAYFISH IN POLAND” 1999-2001

    Directory of Open Access Journals (Sweden)

    ŠMIETANA P.

    2004-01-01

    Full Text Available After the first spread of crayfish plague (aphanomycosis at the end of the nineteenth century, the formerly abundant populations of native crayfish in Poland declined drastically. Analysis of historical data on the occurrence and distribution of crayfish in Poland led to the conclusion that without human intervention the native crayfish species would be at high risk of being made extinct. The largest programme concerning crayfish monitoring and restocking in Poland was “Active protection of native crayfish in Poland” conducted by the Inland Fisheries Institute, the Department of Ecology, University of Szczecin, the Department of Zoology, Warsaw Agricultural University and the University of Warmia and Mazury that was funded by the EkoFundusz Foundation and Inland Fisheries Institute in 1999-2001. One of the main objectives and results of this programme was the determination of indigenous crayfish distribution in Poland (mainly Astacus astacus and Astacus leptodactylus and the restocking of suitable habitats with indigenous crayfish. The densest populations (> 2 500 adult individuals per hectare were used as a source of restocking material. Restocking was performed in 54 water bodies using a total of 45 200 individuals of A. astacus and 9 800 of A. leptodactylus. Between 2001-2002, the success of the restocking programme was monitored. According to the results obtained so far, the restocking exercise has been successful in at least 60.8% of the cases.

  6. KISS1R induces invasiveness of estrogen receptor-negative human mammary epithelial and breast cancer cells.

    Science.gov (United States)

    Cvetkovic, Donna; Dragan, Magdalena; Leith, Sean J; Mir, Zuhaib M; Leong, Hon S; Pampillo, Macarena; Lewis, John D; Babwah, Andy V; Bhattacharya, Moshmi

    2013-06-01

    Kisspeptins (KPs), peptide products of the KISS1 metastasis-suppressor gene, are endogenous ligands for a G protein-coupled receptor (KISS1R). KISS1 acts as a metastasis suppressor in numerous human cancers. However, recent studies have demonstrated that an increase in KISS1 and KISS1R expression in patient breast tumors correlates with higher tumor grade and metastatic potential. We have shown that KP-10 stimulates invasion of estrogen receptor α (ERα)-negative MDA-MB-231 breast cancer cells via transactivation of the epidermal growth factor receptor (EGFR). Here, we report that either KP-10 treatment of ERα-negative nonmalignant mammary epithelial MCF10A cells or expression of KISS1R in MCF10A cells induced a mesenchymal phenotype and stimulated invasiveness. Similarly, exogenous expression of KISS1R in ERα-negative SKBR3 breast cancer cells was sufficient to trigger invasion and induced extravasation in vivo. In contrast, KP-10 failed to transactivate EGFR or stimulate invasiveness in the ERα-positive MCF7 and T47D breast cancer cells. This suggested that ERα negatively regulates KISS1R-dependent breast cancer cell migration, invasion, and EGFR transactivation. In support of this, we found that these KP-10-induced effects were ablated upon exogenous expression of ERα in the MDA-MB-231 cells, by down-regulating KISS1R expression. Lastly, we have identified IQGAP1, an actin cytoskeletal binding protein as a novel binding partner of KISS1R, and have shown that KISS1R regulates EGFR transactivation in breast cancer cells in an IQGAP1-dependent manner. Overall, our data strongly suggest that the ERα status of mammary cells dictates whether KISS1R may be a novel clinical target for treating breast cancer metastasis.

  7. PRR repeats in the intracellular domain of KISS1R are important for its export to cell membrane.

    Science.gov (United States)

    Chevrier, Lucie; de Brevern, Alexandre; Hernandez, Eva; Leprince, Jérome; Vaudry, Hubert; Guedj, Anne Marie; de Roux, Nicolas

    2013-06-01

    Inactivating mutations of KISS-1 receptor (KISS1R) have been recently described as a rare cause of isolated hypogonadotropic hypogonadism transmitted as a recessive trait. Few mutations have been described, and the structure-function relationship of KISS1R remains poorly understood. Here, we have taken advantage of the discovery of a novel mutation of KISS1R to characterize the structure and function of an uncommon protein motif composed of 3 proline-arginine-arginine (PRR) repeats located within the intracellular domain. A heterozygous insertion of 1 PRR repeat in-frame with 3 PRR repeats leading to synthesis of a receptor bearing 4 PRR repeats (PRR-KISS1R) was found in the index case. Functional analysis of PRR-KISS1R showed a decrease of the maximal response to kisspeptin stimulation, associated to a lower cell surface expression without modification of total expression. PRR-KISS1R exerts a dominant negative effect on the synthesis of the wild-type (WT)-KISS1R. This effect was due to the nature of inserted residues but also to the difference of the length of the intracellular domain between PRR-KISS1R and WT-KISS1R. A molecular dynamic analysis showed that the additional PRR constrained this arginine-rich region into a polyproline type II helix. Altogether, this study shows that a heterozygous insertion in KISS1R may lead to hypogonadotropic hypogonadism by a dominant negative effect on the WT receptor. An additional PRR repeat into a proline-arginine-rich motif can dramatically changed the conformation of the intracellular domain of KISS1R and its probable interaction with partner proteins.

  8. A school-based physical activity program to improve health and fitness in children aged 6–13 years ("Kinder-Sportstudie KISS": study design of a randomized controlled trial [ISRCTN15360785

    Directory of Open Access Journals (Sweden)

    Knöpfli Martin

    2006-06-01

    Full Text Available Abstract Background Childhood obesity is the result of a long lasting imbalance between energy intake and energy expenditure. A major contributing factor is physical inactivity which is closely linked to bone health, cardiovascular disease risk, fitness and psychological factors. The school seems to provide an excellent setting to enhance levels of physical activity (PA. However, there is insufficient data from previous school-based intervention trials on how to enhance overall PA. It is also unknown whether an intervention aimed at increasing PA is effective in improving the children's health. The purpose of this paper is to outline the design of a school-based randomized, controlled trial (RCT aiming to increase overall PA and to improve fitness and health in 6- to 13-year-old children. Methods/Design 15 schools were randomized to the intervention (n = 9 or the control (n = 6 group, stratified by geographic region (urban vs. rural and by age (1st and 5th grade. Participation was given for all children in the intervention group since in this group the intervention was part of the normal school curriculum. The intervention during one academic year consisted of: 1. two additional physical education classes per week given by trained physical education teachers adding up to a total of five PA classes per week, 2. short PA breaks (2–5 min each during academic lessons, 3. PA home work, and 4. adaptation of recreational areas around the school. All children underwent anthropometric measurements, blood pressure assessment, fitness testing, measurement of PA and they filled out questionnaires. At least 70% of all children agreed to blood sampling and measurements of body composition and bone mineral measurements by dual energy x-ray absorptiometry. The primary endpoints of the study after one year were an increase in total PA by accelerometry, an increase in aerobic fitness measured by the 20 m shuttle run, a decrease in percent body fat derived from

  9. Finnish bioenergy research programme

    Energy Technology Data Exchange (ETDEWEB)

    Asplund, D. [VTT Energy, Jyvaeskylae (Finland)

    1996-12-31

    Finland is a leading country in the use of biofuels and has excellent opportunities to increase the use of biofuels by up to 25-30 %. The Finnish Government has set an objective for the promotion of bioenergy. The aim is to increase the use of bioenergy by about 25 % from the present level by 2005, and the increment corresponds to 1.5 million tonnes of oil equivalent (toe) per year. The R and D work has been considered as an important factor to achieve this ambitious goal. Energy research was organised into a series of research programmes in 1988 in accordance with the proposal of Finnish Energy Research Committee. The object of the research programmes is to enhance research activities and to bundle individual projects together into larger research packages. The common target of the Finnish energy research programmes is to proceed from basic and applied research to product development and pilot operation, and after that to the first commercial applications, e.g. demonstrations. As the organisation of energy research to programmes has led to good results, the Finnish Ministry of Trade and Industry decided to go on with this practice by launching new six-year programmes in 1993-1998. One of these programmes is the Bioenergy Research Programme and the co-ordination of this programme is carried out by VTT Energy. Besides VTT Energy the Finnish Forest Research Institute, Work Efficiency Institute, Metsaeteho and University of Joensuu are participating in the programme 7 refs.

  10. A cluster-randomised controlled trial of a physical activity and nutrition programme in retirement villages: a study protocol.

    Science.gov (United States)

    Holt, Anne-Marie; Jancey, Jonine; Lee, Andy H; Kerr, Deborah A; Hills, Andrew P; Anderson, Annie S; Howat, Peter A

    2014-09-25

    Physical activity levels of Australia's ageing population are declining and coincidentally rates of overweight and obesity are increasing. Adequate levels of physical activity and a healthy diet are recognised as important lifestyle factors for the maintenance of a healthy weight and prevention of chronic diseases. Retirement village (RV) residents rarely engage in physical activity and nutrition programmes offered, with poor attendance and low use of existing facilities such as on-site fitness centres and classes and nutrition seminars. The RV provides a unique setting to access and engage with this older target group, to test the effectiveness of strategies to increase levels of physical activity, improve nutrition and maintain a healthy weight. This cluster-randomised controlled trial will evaluate a physical activity, nutrition and healthy weight management intervention for insufficiently active ('not achieving 150 min of moderate-intensity physical activity per week') adults aged 60-75 residing in RV's. A total of 400 participants will be recruited from 20 randomly selected RV's in Perth, Western Australia. Villages will be assigned to either the intervention group (n=10) or the control group (n=10) each containing 200 participants. The Retirement Village Physical Activity and Nutrition for Seniors (RVPANS) programme is a home-based physical activity and nutrition programme that includes educational resources, along with facilitators who will motivate and guide the participants during the 6-month intervention. Descriptive statistics and mixed regression models will be performed to assess the intervention effects. This trial will evaluate an intervention for the modification of health risk factors in the RV setting. Such research conducted in RV's has been limited. Curtin University Human Research Ethics Committee (approval number: HR128/2012). Dissemination of the study results will occur through publications, reports, conference presentations and community

  11. KISSing in the History Classroom: Simple Writing Activities That Work.

    Science.gov (United States)

    Kneeshaw, Stephen

    1992-01-01

    Describes a method for integrating writing into history classes. Suggests keeping writing assignments short and simple, rather than assigning a long paper that will be a burden to the instructor during grading. Discusses journal writing, pretest writing exercises, and microthemes written on a single index card. (DK)

  12. The special programme of research in human reproduction: forty years of activities to achieve reproductive health for all.

    Science.gov (United States)

    Benagiano, Giuseppe; d'Arcangues, Catherine; Harris Requejo, Jennifer; Schafer, Alessandra; Say, Lale; Merialdi, Mario

    2012-01-01

    The Special Programme of Research in Human Reproduction (HRP), co-sponsored by the UNDP, UNFPA, WHO, and the World Bank, is celebrating 40 years of activities with an expansion of its mandate and new co-sponsors. When it began, in 1972, the main focus was on evaluating the acceptability, effectiveness, and safety of existing fertility-regulating methods, as well as developing new, improved modalities for family planning. In 1994, HRP not only made major contributions to the Plan of Action of the International Conference on Population and Development (ICPD); it also broadened its scope of work to include other aspects of health dealing with sexuality and reproduction, adding a specific perspective on gender issues and human rights. In 2002, HRP's mandate was once again broadened to include sexually transmitted infections and HIV/AIDS and in 2003 it was further expanded to research activities on preventing violence against women and its many dire health consequences. Today, the work of the Programme includes research on: the sexual and reproductive health of adolescents, women, and men; maternal and perinatal health; reproductive tract and sexually transmitted infections (including HIV/AIDS); family planning; infertility; unsafe abortion; sexual health; screening for cancer of the cervix in developing countries, and gender and reproductive rights. Additional activities by the Programme have included: fostering international cooperation in the field of human reproduction; the elaboration of WHO's first Global Reproductive Health Strategy; work leading to the inclusion of ICPD's goal 'reproductive health for all by 2015' into the Millennium Development Goal framework; the promotion of critical interagency statements on the public health, legal, and human rights implications of female genital mutilation and gender-biased sex selection. Finally, HRP has been involved in the creation of guidelines and tools, such as the 'Medical eligibility criteria for contraceptive use

  13. Research programme 'Active Solar Energy Use - Solar Heating and Heat Storage'. Activities and projects 2003; Programme 'Solaire actif - Chaleur et Stockage de chaleur'. Activites et projets en 2003

    Energy Technology Data Exchange (ETDEWEB)

    Hadorn, J.-C. [Base Consultants, Geneva (Switzerland); Renaud, P. [Planair SA, La Sagne (Switzerland)

    2003-07-01

    In this report by the research, development and demonstration (RD+D) programme coordinators the objectives, activities and main results in the area of solar heating and heat storage in Switzerland are presented for 2003. In a stagnating market environment the strategy of the Swiss Federal Office of Energy mainly consists in improving the quality and durability of solar collectors and materials, optimizing combisystems for space heating and domestic hot water preparation, searching for storage systems with a higher energy storage density than in the case of sensible heat storage in water, developing coloured solar collectors for more architectonic freedom, and finalizing a seasonal heat storage project for 100 dwellings to demonstrate the feasibility of solar fractions larger than 50% in apartment houses. Support was granted to the Swiss Testing Facility SPF in Rapperswil as in previous years; SPF was the first European testing institute to perform solar collector labeling according to the new rules of the 'Solar Keymark', introduced in cooperation with the European Committee for Standardization CEN. Several 2003 projects were conducted within the framework of the Solar Heating and Cooling Programme of the International Energy Agency IEA. Computerized simulation tools were improved. With the aim of jointly producing high-temperature heat and electric power a solar installation including a concentrating collector and a thermodynamic machine based on a Rankine cycle is still being developed. Seasonal underground heat storage was studied in detail by means of a validated computer simulation programme. Design guidelines were obtained for such a storage used in the summer time for cooling and in the winter time for space heating via a heat pump: depending on the ratio 'summer cooling / winter heating', cooling requires a cooling machine, or direct cooling without such a machine is possible. The report ends up with the list of all supported RD

  14. Free-roaming kissing bugs, vectors of Chagas disease, feed often on humans in the Southwest.

    Science.gov (United States)

    Klotz, Stephen A; Schmidt, Justin O; Dorn, Patricia L; Ivanyi, Craig; Sullivan, Katherine R; Stevens, Lori

    2014-05-01

    Kissing bugs, vectors of Trypanosoma cruzi, the parasite that causes Chagas disease, are common in the desert Southwest. After a dispersal flight in summer, adult kissing bugs occasionally gain access to houses where they remain feeding on humans and pets. How often wild, free-roaming kissing bugs feed on humans outside their homes has not been studied. This is important because contact of kissing bugs with humans is one means of gauging the risk for acquisition of Chagas disease. We captured kissing bugs in a zoological park near Tucson, Arizona, where many potential vertebrate hosts are on display, as well as being visited by more than 300,000 humans annually. Cloacal contents of the bugs were investigated for sources of blood meals and infection with T. cruzi. Eight of 134 captured bugs were randomly selected and investigated. All 8 (100%) had human blood in their cloacae, and 7 of 8 (88%) had fed on various vertebrates on display or feral in the park. Three bugs (38%) were infected with T. cruzi. Three specimens of the largest species of kissing bug in the United States (Triatoma recurva) were captured in a cave and walking on a road; 2 of 3 (67%) had fed on humans. No T. recurva harbored T. cruzi. This study establishes that free-roaming kissing bugs, given the opportunity, frequently feed on humans outside the confines of their homes in the desert Southwest and that some harbored T. cruzi. This could represent a hitherto unrecognized potential for transmission of Chagas disease in the United States. Copyright © 2014 Elsevier Inc. All rights reserved.

  15. Efficacy of a compulsory homework programme for increasing physical activity and healthy eating in children: the healthy homework pilot study

    Directory of Open Access Journals (Sweden)

    Duncan Scott

    2011-11-01

    Full Text Available Abstract Background Most physical activity and nutrition interventions in children focus on the school setting; however, evidence suggests that children are less active and have greater access to unhealthy food at home. The aim of this pilot study was to examine the efficacy of a compulsory homework programme for increasing physical activity and healthy eating in children. Methods The six-week 'Healthy Homework' programme and complementary teaching resource was developed under the guidance of an intersectoral steering group. Eight senior classes (year levels 5-6 from two diverse Auckland primary schools were randomly assigned into intervention and control groups. A total of 97 children (57 intervention, 40 control aged 9-11 years participated in the evaluation of the intervention. Daily step counts were monitored immediately before and after the intervention using sealed multiday memory pedometers. Screen time, sports participation, active transport to and from school, and the consumption of fruits, vegetables, unhealthy foods and drinks were recorded concurrently in a 4-day food and activity diary. Results Healthy Homework resulted in a significant intervention effect of 2,830 steps.day-1 (95% CI: 560, 5,300, P = 0.013. This effect was consistent between sexes, schools, and day types (weekdays and weekend days. In addition, significant intervention effects were observed for vegetable consumption (0.83 servings.day-1, 95% CI: 0.24, 1.43, P = 0.007 and unhealthy food consumption (-0.56 servings.day-1, 95% CI: -1.05, -0.07, P = 0.027 on weekends but not weekdays, with no interactions with sex or school. Effects for all other variables were not statistically significant regardless of day type. Conclusions Compulsory health-related homework appears to be an effective approach for increasing physical activity and improving vegetable and unhealthy food consumption in children. Further research in a larger study is required to confirm these initial

  16. The ACIGA data analysis programme

    Energy Technology Data Exchange (ETDEWEB)

    Scott, Susan M; Searle, Antony C; Cusack, Benedict J; McClelland, David E [Department of Physics, Faculty of Science, Australian National University, Canberra ACT 0200 (Australia)

    2004-03-07

    The data analysis programme of the Australian Consortium for Interferometric Gravitational Astronomy (ACIGA) was set up in 1998 by Scott to complement the then existing ACIGA programmes working on suspension systems, lasers and optics and detector configurations. The ACIGA data analysis programme continues to contribute significantly in the field; we present an overview of our activities.

  17. The ACIGA Data Analysis programme

    CERN Document Server

    Scott, S M; Cusack, B J; McClelland, D E; Scott, Susan M; Searle, Antony C; Cusack, Benedict J; Clelland, David E Mc

    2004-01-01

    The Data Analysis programme of the Australian Consortium for Interferometric Gravitational Astronomy (ACIGA) was set up in 1998 by the first author to complement the then existing ACIGA programmes working on suspension systems, lasers and optics, and detector configurations. The ACIGA Data Analysis programme continues to contribute significantly in the field; we present an overview of our activities.

  18. Genome-scale definition of the transcriptional programme associated with compromised PU.1 activity in acute myeloid leukaemia.

    Science.gov (United States)

    Sive, J I; Basilico, S; Hannah, R; Kinston, S J; Calero-Nieto, F J; Göttgens, B

    2016-01-01

    Transcriptional dysregulation is associated with haematological malignancy. Although mutations of the key haematopoietic transcription factor PU.1 are rare in human acute myeloid leukaemia (AML), they are common in murine models of radiation-induced AML, and PU.1 downregulation and/or dysfunction has been described in human AML patients carrying the fusion oncogenes RUNX1-ETO and PML-RARA. To study the transcriptional programmes associated with compromised PU.1 activity, we adapted a Pu.1-mutated murine AML cell line with an inducible wild-type PU.1. PU.1 induction caused transition from leukaemia phenotype to monocytic differentiation. Global binding maps for PU.1, CEBPA and the histone mark H3K27Ac with and without PU.1 induction showed that mutant PU.1 retains DNA-binding ability, but the induction of wild-type protein dramatically increases both the number and the height of PU.1-binding peaks. Correlating chromatin immunoprecipitation (ChIP) Seq with gene expression data, we found that PU.1 recruitment coupled with increased histone acetylation induces gene expression and activates a monocyte/macrophage transcriptional programme. PU.1 induction also caused the reorganisation of a subgroup of CEBPA binding peaks. Finally, we show that the PU.1 target gene set defined in our model allows the stratification of primary human AML samples, shedding light on both known and novel AML subtypes that may be driven by PU.1 dysfunction.

  19. THE FINANCING OF THE ACTIVITIES WITHIN THE SECTORIAL OPERATIONAL PROGRAMME “INCREASE OF ECONOMIC COMPETITIVENESS”

    Directory of Open Access Journals (Sweden)

    Vasile POPEANGĂ

    2009-12-01

    Full Text Available The Sectorial Operational Programme “Increase of Economic Competitiveness” (further referred to as SOP IEC is one of the seven instruments (OPs, under the Convergence objective, for achieving the priorities of the National Strategic Reference Framework (NSRF derived from the National Development Plan 2007 – 2013 (NDP, which aims to strengthen the strategic focus of the Economic and Social Cohesion policies across Romania, and to make the correct and appropriate linkages to the European policies and the Lisbon Strategy for growth and job creation. SOP IEC was elaborated under the coordination of the Managing Authority for SOP IEC -Ministry of Economy and Finance (MEF, and is the result of the partnership consultations both with the strategic partners (ACIS within MEF, other MAs–ministries coordinating other OPs, institutions designated as Intermediate Bodies, other line ministries, and agencies, social partners, civil society organizations, potential beneficiaries, other stakeholders involved in this field.

  20. Field programmable gate array based data digitisation with commercial elements

    Energy Technology Data Exchange (ETDEWEB)

    Ugur, Cahit; Koenig, Wolfgang; Traxler, Michael [GSI Helmholtz Centre for Heavy Ion Research (Germany); Michel, Jan [Goethe Univ. Frankfurt am Main (Germany); Korcyl, Grzegorz; Palka, Marek [Jagiellonian University (Poland)

    2013-07-01

    One of the most important aspects of particle identification experiments is the digitisation of time, amplitude and charge data from detectors. These conversions are done mostly with Application Specific ICs (ASICs). However, the recent developments in Field Programmable Gate Array (FPGA) technology allow us to use commercial electronic components for the required Front-End Electronics (FEE) and do the digitisation in the FPGA. It is possible to do Time-of-Flight (ToF), Time-over-Threshold (ToT), amplitude and charge measurements with converters implemented in FPGA. We call this principle COME and KISS: Use COMplex COMmercial Elements and Keep It Small and Simple.

  1. Field programmable gate array based data digitisation with commercial elements

    Science.gov (United States)

    Ugur, C.; Koening, W.; Michel, J.; Palka, M.; Traxler, M.

    2013-01-01

    One of the most important aspects of particle identification experiments is the digitisation of time, amplitude and charge data from detectors. These conversions are mostly undertaken with Application Specific Integrated Circuits (ASICs). However, recent developments in Field Programmable Gate Array (FPGA) technology allow us to use commercial electronic components for the required Front-End Electronics (FEE) and to do the digitisation in the FPGA. It is possible to do Time-of-Flight (ToF), Time-over-Threshold (ToT), amplitude and charge measurements with converters implemented in FPGA. We call this principle come & kiss: use COmplex ComMErcial Elements & Keep It Small and Simple.

  2. Two families with normosmic congenital hypogonadotropic hypogonadism and biallelic mutations in KISS1R (KISS1 receptor: clinical evaluation and molecular characterization of a novel mutation.

    Directory of Open Access Journals (Sweden)

    Frédéric Brioude

    Full Text Available CONTEXT: KISS1R mutations have been reported in few patients with normosmic congenital hypogonadotropic hypogonadism (nCHH (OMIM #146110. OBJECTIVE: To describe in detail nCHH patients with biallelic KISS1R mutations belonging to 2 unrelated families, and to functionally characterize a novel KISS1R mutation. RESULTS: An original mutant, p.Tyr313His, was found in the homozygous state in 3 affected kindred (2 females and 1 male from a consanguineous Portuguese family. This mutation, located in the seventh transmembrane domain, affects a highly conserved amino acid, perturbs the conformation of the transmembrane segment, and impairs MAP kinase signaling and intracellular calcium release. In the second family, a French Caucasian male patient with nCHH was found to carry two recurrent mutations in the compound heterozygous state (p.Leu102Pro/Stop399Arg. In this man, pulsatile GnRH (Gonadotropin Releasing Hormone administration restored pulsatile LH (Luteinizing Hormone secretion and testicular hormone secretion. Later, long-term combined gonadotropin therapy induced spermatogenesis, enabling 3 successive pregnancies that resulted in 2 miscarriages and the birth of a healthy boy. CONCLUSION: We show that a novel loss-of-function mutation (p.Tyr313His in the KISS1R gene can cause familial nCHH, revealing the crucial role of this amino acid in KISS1R function. The observed restoration of gonadotropin secretion by exogenous GnRH administration further supports, in humans, the hypothalamic origin of the gonadotropin deficiency in this genetic form of nCHH.

  3. Assessing the Financial Viability of Academic Programmes

    Science.gov (United States)

    Swift, Lynette

    2012-01-01

    This paper reviews and examines approaches to determining the financial viability of academic programmes as a critical component of assessing a programme's overall sustainability. Key to assessing the financial viability of a programme is understanding the teaching activities required to deliver the programme and the cost of those activities. A…

  4. DNA–DNA kissing complexes as a new tool for the assembly of DNA nanostructures

    Science.gov (United States)

    Barth, Anna; Kobbe, Daniela; Focke, Manfred

    2016-01-01

    Kissing-loop annealing of nucleic acids occurs in nature in several viruses and in prokaryotic replication, among other circumstances. Nucleobases of two nucleic acid strands (loops) interact with each other, although the two strands cannot wrap around each other completely because of the adjacent double-stranded regions (stems). In this study, we exploited DNA kissing-loop interaction for nanotechnological application. We functionalized the vertices of DNA tetrahedrons with DNA stem-loop sequences. The complementary loop sequence design allowed the hybridization of different tetrahedrons via kissing-loop interaction, which might be further exploited for nanotechnology applications like cargo transport and logical elements. Importantly, we were able to manipulate the stability of those kissing-loop complexes based on the choice and concentration of cations, the temperature and the number of complementary loops per tetrahedron either at the same or at different vertices. Moreover, variations in loop sequences allowed the characterization of necessary sequences within the loop as well as additional stability control of the kissing complexes. Therefore, the properties of the presented nanostructures make them an important tool for DNA nanotechnology. PMID:26773051

  5. Ecological patterns of blood-feeding by kissing-bugs (Hemiptera: Reduviidae: Triatominae).

    Science.gov (United States)

    Rabinovich, Jorge Eduardo; Kitron, Uriel Dan; Obed, Yamila; Yoshioka, Miho; Gottdenker, Nicole; Chaves, Luis Fernando

    2011-06-01

    Host use by vectors is important in understanding the transmission of zoonotic diseases, which can affect humans, wildlife and domestic animals. Here, a synthesis of host exploitation patterns by kissing-bugs, vectors of Chagas disease, is presented. For this synthesis, an extensive literature review restricted to feeding sources analysed by precipitin tests was conducted. Modern tools from community ecology and multivariate statistics were used to determine patterns of segregation in host use. Rather than innate preferences for host species, host use by kissing-bugs is influenced by the habitats they colonise. One of the major limitations of studies on kissing-bug foraging has been the exclusive focus on the dominant vector species. We propose that expanding foraging studies to consider the community of vectors will substantially increase the understanding of Chagas disease transmission ecology. Our results indicate that host accessibility is a major factor that shapes the blood-foraging patterns of kissing-bugs. Therefore, from an applied perspective, measures that are directed at disrupting the contact between humans and kissing-bugs, such as housing improvement, are among the most desirable strategies for Chagas disease control.

  6. Vascular angular remodeling by kissing-Y stenting in wide necked intracranial bifurcation aneurysms.

    Science.gov (United States)

    Melber, Katharina; Meila, Dan; Draheim, Philipp; Grieb, Dominik; Greling, Björn; Schlunz-Hendann, Martin; Brassel, Friedhelm

    2016-12-05

    Single stent deployment leads to a change in vascular geometry in wide necked bifurcation aneurysms. In some complex cases, the use of the single stent technique might not be sufficient or may not be feasible. The kissing-Y stenting technique appears to be an alternative endovascular treatment option. The aim of this study was to evaluate the effects of the kissing-Y stenting technique on vascular angular remodeling. 21 patients with wide necked intracranial bifurcation aneurysms at different sites (10 anterior communicating artery, 6 middle cerebral artery, 3 basilar artery, 1 vertebral artery/posterior inferior cerebellar artery, 1 internal carotid artery/posterior communicating artery) were treated with 44 closed cell stents (follow-up 2012-2016) using the kissing-Y stenting technique. We analyzed vascular angle geometry between the mother and both affected daughter vessels by digital subtraction angiography, before and after stent deployment, using standard working projections. Endovascular treatment of wide necked intracranial aneurysms using the kissing-Y stenting technique significantly decreased the angle between the bifurcation branches from 130.4±9.5° to 91.5±9.1° (pKissing-Y stenting in wide necked bifurcation aneurysms leads to vascular angular remodeling of both affected branches. The resulting straightening of the bifurcation angle may prevent aneurysmal recurrence. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

  7. Ecological patterns of blood-feeding by kissing-bugs (Hemiptera: Reduviidae: Triatominae

    Directory of Open Access Journals (Sweden)

    Jorge Eduardo Rabinovich

    2011-06-01

    Full Text Available Host use by vectors is important in understanding the transmission of zoonotic diseases, which can affect humans, wildlife and domestic animals. Here, a synthesis of host exploitation patterns by kissing-bugs, vectors of Chagas disease, is presented. For this synthesis, an extensive literature review restricted to feeding sources analysed by precipitin tests was conducted. Modern tools from community ecology and multivariate statistics were used to determine patterns of segregation in host use. Rather than innate preferences for host species, host use by kissing-bugs is influenced by the habitats they colonise. One of the major limitations of studies on kissing-bug foraging has been the exclusive focus on the dominant vector species. We propose that expanding foraging studies to consider the community of vectors will substantially increase the understanding of Chagas disease transmission ecology. Our results indicate that host accessibility is a major factor that shapes the blood-foraging patterns of kissing-bugs. Therefore, from an applied perspective, measures that are directed at disrupting the contact between humans and kissing-bugs, such as housing improvement, are among the most desirable strategies for Chagas disease control.

  8. A kissing loop is important for btuB riboswitch ligand sensing and regulatory control.

    Science.gov (United States)

    Lussier, Antony; Bastet, Laurène; Chauvier, Adrien; Lafontaine, Daniel A

    2015-10-30

    RNA-based genetic regulation is exemplified by metabolite-binding riboswitches that modulate gene expression through conformational changes. Crystal structures show that the Escherichia coli btuB riboswitch contains a kissing loop interaction that is in close proximity to the bound ligand. To analyze the role of the kissing loop interaction in the riboswitch regulatory mechanism, we used RNase H cleavage assays to probe the structure of nascent riboswitch transcripts produced by the E. coli RNA polymerase. By monitoring the folding of the aptamer, kissing loop, and riboswitch expression platform, we established the conformation of each structural component in the absence or presence of bound adenosylcobalamin. We found that the kissing loop interaction is not essential for ligand binding. However, we showed that kissing loop formation improves ligand binding efficiency and is required to couple ligand binding to the riboswitch conformational changes involved in regulating gene expression. These results support a mechanism by which the btuB riboswitch modulates the formation of a tertiary structure to perform metabolite sensing and regulate gene expression. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  9. DNA-DNA kissing complexes as a new tool for the assembly of DNA nanostructures.

    Science.gov (United States)

    Barth, Anna; Kobbe, Daniela; Focke, Manfred

    2016-02-29

    Kissing-loop annealing of nucleic acids occurs in nature in several viruses and in prokaryotic replication, among other circumstances. Nucleobases of two nucleic acid strands (loops) interact with each other, although the two strands cannot wrap around each other completely because of the adjacent double-stranded regions (stems). In this study, we exploited DNA kissing-loop interaction for nanotechnological application. We functionalized the vertices of DNA tetrahedrons with DNA stem-loop sequences. The complementary loop sequence design allowed the hybridization of different tetrahedrons via kissing-loop interaction, which might be further exploited for nanotechnology applications like cargo transport and logical elements. Importantly, we were able to manipulate the stability of those kissing-loop complexes based on the choice and concentration of cations, the temperature and the number of complementary loops per tetrahedron either at the same or at different vertices. Moreover, variations in loop sequences allowed the characterization of necessary sequences within the loop as well as additional stability control of the kissing complexes. Therefore, the properties of the presented nanostructures make them an important tool for DNA nanotechnology. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  10. A Kissing Loop Is Important for btuB Riboswitch Ligand Sensing and Regulatory Control*

    Science.gov (United States)

    Lussier, Antony; Bastet, Laurène; Chauvier, Adrien; Lafontaine, Daniel A.

    2015-01-01

    RNA-based genetic regulation is exemplified by metabolite-binding riboswitches that modulate gene expression through conformational changes. Crystal structures show that the Escherichia coli btuB riboswitch contains a kissing loop interaction that is in close proximity to the bound ligand. To analyze the role of the kissing loop interaction in the riboswitch regulatory mechanism, we used RNase H cleavage assays to probe the structure of nascent riboswitch transcripts produced by the E. coli RNA polymerase. By monitoring the folding of the aptamer, kissing loop, and riboswitch expression platform, we established the conformation of each structural component in the absence or presence of bound adenosylcobalamin. We found that the kissing loop interaction is not essential for ligand binding. However, we showed that kissing loop formation improves ligand binding efficiency and is required to couple ligand binding to the riboswitch conformational changes involved in regulating gene expression. These results support a mechanism by which the btuB riboswitch modulates the formation of a tertiary structure to perform metabolite sensing and regulate gene expression. PMID:26370077

  11. Single-molecule observations of RNA-RNA kissing interactions in a DNA nanostructure.

    Science.gov (United States)

    Takeuchi, Yosuke; Endo, Masayuki; Suzuki, Yuki; Hidaka, Kumi; Durand, Guillaume; Dausse, Eric; Toulmé, Jean-Jacques; Sugiyama, Hiroshi

    2016-01-01

    RNA molecules uniquely form a complex through specific hairpin loops, called a kissing complex. The kissing complex is widely investigated and used for the construction of RNA nanostructures. Molecular switches have also been created by combining a kissing loop and a ligand-binding aptamer to control the interactions of RNA molecules. In this study, we incorporated two kinds of RNA molecules into a DNA origami structure and used atomic force microscopy to observe their ligand-responsive interactions at the single-molecule level. We used a designed RNA aptamer called GTPswitch, which has a guanosine triphosphate (GTP) responsive domain and can bind to the target RNA hairpin named Aptakiss in the presence of GTP. We observed shape changes of the DNA/RNA strands in the DNA origami, which are induced by the GTPswitch, into two different shapes in the absence and presence of GTP, respectively. We also found that the switching function in the nanospace could be improved by using a cover strand over the kissing loop of the GTPswitch or by deleting one base from this kissing loop. These newly designed ligand-responsive aptamers can be used for the controlled assembly of the various DNA and RNA nanostructures.

  12. Kissing pleural metastases from metastatic osteosarcoma of the lung.

    Science.gov (United States)

    Mori, Takeshi; Yoshioka, Masakazu; Iwatani, Kazunori; Kobayashi, Hironori; Yoshimoto, Kentaro; Nomori, Hiroaki

    2006-04-01

    Two patients with osteosarcoma lung metastases of which migrated to the parietal pleura due to contact are reported. The first patient was a 16-year-old male who had a pleural metastasis in the diaphragm within an area in contact with a single lung metastasis. Both of the tumors were resected, followed by systemic chemotherapy. Nine months after the resection of the first metastases, two other lung metastases were found which were resected after chemotherapy. The patient is alive without recurrence 84 months after the first resection of the metastases. The second patient was an 11-year-old female with a pleural metastasis of osteosarcoma which was within an area in contact with a single lung metastasis, which had been resected 4 months before. We concluded (1) that a lung metastasis of osteosarcoma occasionally metastasizes to the pleura due to contact; and (2) that because this kissing metastases of osteosarcoma could be cured by a complete resection, the intrathoracic cavity should be thoroughly observed.

  13. Broadband nano-focusing of light using kissing nanowires

    Energy Technology Data Exchange (ETDEWEB)

    Lei, Dang Yuan; Aubry, Alexandre; Maier, Stefan A; Pendry, John B, E-mail: dangyuan.lei08@imperial.ac.u [Blackett Laboratory, Department of Physics, Imperial College London, London SW7 2AZ (United Kingdom)

    2010-09-15

    A strategy has been proposed recently to design plasmonic nanostructures capable of efficient harvesting of light over a broadband spectrum. Applying a singular conformal transformation to a metal-insulator-metal infinite structure, the optical response of two kissing nanowires can be deduced analytically. This nanostructure is shown to exhibit a large and continuous absorption cross-section relative to its physical size over the whole visible spectrum. Considerable field enhancement and confinement at the nano-scale are also expected at the touching point. Actually, instead of transporting the energy out to infinity, like in a metal slab geometry, the surface plasmon modes here propagate towards the singularity of the structure where their velocity vanishes and energy accumulates. The field enhancement is then a balance between this energy accumulation and dissipation losses. The asymptotic case of a nanowire placed on top of a metal plate is shown to be of great interest for nanofocusing. Finally, numerical simulations are performed to investigate the effect of radiative losses when the structure dimension becomes comparable to the wavelength.

  14. REHABILITATION PRACTICAL PROGRAMME

    Directory of Open Access Journals (Sweden)

    Suzana KRANJC JOLDIKJ

    Full Text Available Centre for Education and Rehabilitation of Physi­cally Handicapped Children and Adolescents Kamnik (Zavod za usposabljanje invalidne mlad­ine Kamnik; hereinafter: ZUIM perform verified or state-ap­proved programme the Rehabilitation practical pro­gramme. The programme is intended for all those young people, who have completed primary school education, but cannot continue regular schooling in secondary school pro­grammes. The programme con­sists of several equivalent parts: education, practical work, train­ing work, health, therapeutic, psychologi­cal, and other activities. For every beginner in the first month of education members of the operative team create an individualized programme, which in­cludes individualized school work, individualized training programme, and other expert activities. The programme can last for 6 years maximum, it can however be completed earlier, when the op­erative team feels the training is no longer neces­sary. Pro­gress of a young person is what matters the most, and if there is no progress, the training is brought to an end. Training of young people in the Rehabilitation practical programme is only the be­ginning. The country will have to start considering social enter­prises, which are found elsewhere in the world, for example in Scandinavian countries and in the USA.

  15. Endovascular coil embolization for ruptured kissing aneurysms associated with A1 fenestration.

    Science.gov (United States)

    Mitsuhara, Takafumi; Sakamoto, Shigeyuki; Kiura, Yoshihiro; Kurisu, Kaoru

    2011-01-01

    Fenestration of intracranial arteries is a rare anomaly, and is frequently associated with cerebral aneurysms. In this paper, we report rare kissing aneurysms associated with A1 fenestration. A 71-year-old woman presented with subarachnoid hemorrhage. Diagnostic digital subtraction angiography revealed two saccular aneurysms at the proximal junction of a fenestration and posterior aspect of the fenestration that appeared to be 'kissing' each other. Emergent endovascular coil embolization was performed. Kissing aneurysms associated with fenestration of the horizontal segment in the anterior cerebral artery are rare, and have not been reported. During treatment of such specific types of aneurysms by endovascular treatment, three-dimensional rotational digital subtraction angiography was very useful for deciding the appropriate working angles.

  16. Nuclear spectroscopy of r-process nuclei around N = 126 using KISS

    Science.gov (United States)

    Hirayama, Y.; Watanabe, Y. X.; Miyatake, H.; Schury, P.; Wada, M.; Oyaizu, M.; Kakiguchi, Y.; Mukai, M.; Kimura, S.; Ahmed, M.; Jeong, S. C.; Moon, J. Y.; Park, J. H.

    2017-09-01

    The beta-decay properties and atomic mass of nuclei with neutron magic number of N = 126 are considered critical for understanding the production of heavy elements such as gold and platinum at astrophysical sites. We will produce and measure the half-lives and masses of the nuclei with Z = 74-77 around N = 126 by using the multinucleon transfer (MNT) reaction of ^{136} Xe/ ^{238} U beams and ^{198} Pt target system. For this purpose, we have constructed the KEK Isotope Separation System (KISS) at RIKEN RIBF facility. KISS consists of an argon gas cell based laser ion source (atomic number selection) and an isotope separation on-line (ISOL) (mass number selection), to produce pure low-energy beams of neutron-rich isotopes around N = 126 . We performed the on-line tests to study the basic properties of the KISS and, successfully extracted laser-ionized nuclei around N = 126.

  17. Hypothalamic kiss1 mRNA and kisspeptin immunoreactivity are reduced in a rat model of polycystic ovary syndrome (PCOS).

    Science.gov (United States)

    Brown, Russell E; Wilkinson, Diane A; Imran, Syed A; Caraty, Alain; Wilkinson, Michael

    2012-07-27

    An intact hypothalamic kiss1/kisspeptin/kiss1r complex is a prerequisite for reproductive competence, and kisspeptin treatment could be a practical therapeutic approach to some problems of infertility. One such disorder is polycystic ovarian syndrome (PCOS), a common cause of infertility affecting more than 100 million women. A rodent model of PCOS is the prepubertal female rat treated for a prolonged period with dihydrotestosterone (DHT), which induces many of the metabolic characteristics of the syndrome. We hypothesized that hypothalamic kiss1 mRNA levels, and kisspeptin immunoreactivity (ir), would be abnormal in these rats. Prepubertal female rats were exposed to DHT for 60 days. Rats were killed in two groups: at 26 and 60 days of DHT exposure. Kiss1 mRNA was quantified in hypothalamus, pituitary, ovary and visceral adipose tissue. Separate groups of rats provided brain tissue for immunohistochemical analysis of kisspeptin-ir. At 26 days of DHT exposure, hypothalamic kiss1 mRNA was severely depleted. In contrast DHT had no effect on pituitary kiss1 expression but it significantly increased levels of kiss1 mRNA in fat (+9-fold; povary (+3-fold; povary but remained elevated in fat (+4-fold; ppolycystic ovary syndrome. In hypothalamus, specifically, kiss1 mRNA, and levels of kisspeptin immunoreactivity, are significantly reduced. Since these rats exhibit many of the characteristics of polycystic ovary syndrome, we suggest that atypical kiss1 expression may contribute to the multiple tissue abnormalities observed in women with this disorder. However, and of some importance, our data do not appear to be consistent with the elevated levels of LH seen in women with PCOS; i.e. reduced levels of hypothalamic kiss1 mRNA and kisspeptin immunoreactivity observed in DHT-treated rats are unlikely to produce elevated LH secretion.

  18. A structured physical activity and fitness programme for older adults with intellectual disabilities : Results of a cluster-randomised clinical trial

    NARCIS (Netherlands)

    van Schijndel-Speet, M.; Evenhuis, Heleen M.; van Wijck, R.; van Montfort, K. C. A. G. M.; Echteld, M. A.

    BackgroundThe physical activity level of older adults with intellectual disabilities (ID) is extremely low, and their fitness levels are far beneath accepted norms for older people with normal intelligence and comparable with frail older people. A physical activity programme, including an education

  19. The I.A.G. / A.I.G. SEDIBUD (Sediment Budgets in Cold Environments) Programme: Current and future activities

    Science.gov (United States)

    Beylich, Achim A.; Lamoureux, Scott; Decaulne, Armelle

    2013-04-01

    Projected climate change in cold regions is expected to alter melt season duration and intensity, along with the number of extreme rainfall events, total annual precipitation and the balance between snowfall and rainfall. Similarly, changes to the thermal balance are expected to reduce the extent of permafrost and seasonal ground frost and increase active layer depths. These effects will undoubtedly change surface environments in cold regions and alter the fluxes of sediments, nutrients and solutes, but the absence of quantitative data and coordinated geomorphic process monitoring and analysis to understand the sensitivity of the Earth surface environment is acute in cold climate environments. The International Association of Geomorphologists (I.A.G. / A.I.G. ) SEDIBUD (Sediment Budgets in Cold Environments) Programme was formed in 2005 to address this existing key knowledge gap. SEDIBUD currently has about 400 members worldwide and the Steering Committee of this international programme is composed of ten scientists from eight different countries: Achim A. Beylich (Chair) (Norway), Armelle Decaulne (Secretary) (France), John C. Dixon (USA), Scott F. Lamoureux (Vice-Chair) (Canada), John F. Orwin (Canada), Jan-Christoph Otto (Austria), Irina Overeem (USA), Thorsteinn Sæmundsson (Iceland), Jeff Warburton (UK) and Zbigniew Zwolinski (Poland). The central research question of this global group of scientists is to: Assess and model the contemporary sedimentary fluxes in cold climates, with emphasis on both particulate and dissolved components. Initially formed as European Science Foundation (ESF) Network SEDIFLUX (Sedimentary Source-to-Sink Fluxes in Cold Environments) (2004 - ), SEDIBUD has further expanded to a global group of researchers with field research sites located in polar and alpine regions in the northern and southern hemisphere. Research carried out at each of the close to 50 defined SEDIBUD key test sites varies by programme, logistics and available

  20. Urgent endovascular treatment of proximal right subclavian artery pseudoaneurysm using kissing technique.

    Science.gov (United States)

    Zhang, Mi; Yuan, Ye; Hu, Yali; Zhao, Yi; Liu, Hui; Lu, Huijun

    2015-08-01

    A 50-year old man, sustained a blunt force injury to the chest during an engineering accident, underwent urgent endovascular angioplasty to treat proximal right subclavian artery pseudoaneurysm (RSAP) with a hostile neck in our hospital. Six-month follow-up showed that there was sufficient right carotid artery and right subclavian artery infusion, and the kissing stents were patent. The results show the safety and efficacy of kissing stent technique for RSAP in emergency patients who has no sufficient proximal fixation zone. Copyright © 2015 Elsevier Inc. All rights reserved.

  1. Characteristics of the kissing-bond in friction stir welded Al alloy 1050

    Energy Technology Data Exchange (ETDEWEB)

    Sato, Yutaka S. [Department of Materials Processing, Graduate School of Engineering, Tohoku University, 6-6-02 Aramaki-aza-Aoba, Sendai 980-8579 (Japan)]. E-mail: ytksato@material.tohoku.ac.jp; Takauchi, Hideaki [Department of Materials Processing, Graduate School of Engineering, Tohoku University, 6-6-02 Aramaki-aza-Aoba, Sendai 980-8579 (Japan); Park, Seung Hwan C. [Department of Materials Processing, Graduate School of Engineering, Tohoku University, 6-6-02 Aramaki-aza-Aoba, Sendai 980-8579 (Japan); Kokawa, Hiroyuki [Department of Materials Processing, Graduate School of Engineering, Tohoku University, 6-6-02 Aramaki-aza-Aoba, Sendai 980-8579 (Japan)

    2005-09-25

    Initial oxide layer on the butt surface fragments during friction stir welding (FSW) often remaining as a faint zigzag-line pattern on the cross section. When remnants of the oxide layer often adversely affects the mechanical properties in the weld, it is called as 'kissing-bond'. The present study systematically examines the effect of oxide array on the bend property in the root region of friction stir (FS) welded Al alloy 1050 by transmission electron microscopy (TEM) to clarify the identity of 'kissing-bond'.

  2. SWOV programme 2003-2006.

    NARCIS (Netherlands)

    2012-01-01

    SWOV uses a four-year programme to structure its activities over a longer period of time. The current four-year programme, for 2003-2006, includes proposals for so-called planning office activities, anticipatory research, knowledge management, and knowledge dissemination. The research activities are

  3. Hardware Activation by Means of PUFs and Elliptic Curve Cryptography in Field-Programmable Devices

    Directory of Open Access Journals (Sweden)

    Luis Parrilla

    2016-01-01

    Full Text Available Reusable design using IP cores requires of efficient methods for protecting the Intellectual Property of the designer and the corresponding license agreements. In this work, a new protection procedure establishing an activation protocol in a similar way to the activation process in the software world is presented. The procedure, named SEHAS (Secure Hardware Activation System allows the distribution of cores in either Blocked (not functioning or Demo (functioning with limited features modes, while ensuring the license agreements by identifying not only the IP core but also the implementation device, using Physically Unclonable Functions (PUF. Moreover, SEHAS secures the exchange of information between the core and the core vendor using an Elliptic Curve Cryptosystem (ECC. This secure channel allows the IP core vendor to send a unique Activation Code to the core in order to switch it to the Activated Mode, thus enabling all its features.

  4. Origami-inspired active graphene-based paper for programmable instant self-folding walking devices

    OpenAIRE

    Mu, Jiuke; Hou, Chengyi; Wang, Hongzhi; Li, Yaogang; Zhang, Qinghong; Zhu, Meifang

    2015-01-01

    Origami-inspired active graphene-based paper with programmed gradients in vertical and lateral directions is developed to address many of the limitations of polymer active materials including slow response and violent operation methods. Specifically, we used function-designed graphene oxide as nanoscale building blocks to fabricate an all-graphene self-folding paper that has a single-component gradient structure. A functional device composed of this graphene paper can (i) adopt predesigned sh...

  5. Greek Teachers Programme 2015

    CERN Multimedia

    Hoch, Michael

    2015-01-01

    The 3rd edition of this year's Greek Teachers Programme was co-organized by CERN Education Group and the Hellenic Physical Society and took place from 8 to 12 November 2015. The programme targets physics high-school teachers from all over Greece. It aims to help teachers inspire the next generation of scientists and engineers by motivating their students to understand and appreciate how science works at the world's largest physics laboratory, whereby increasing their interest in pursuing studies in STEM fields in secondary and post-secondary education. 33 teachers took part in this programme which comprised lectures by Greek members of the CERN scientific community, with visits to experimental facilities, hands-on activities and dedicated sessions on effective and creative ways through which participants may bring physics, particle physics and CERN closer to their school classroom. In 2015, more than 100 teachers took part in the three editions of the Greek Teachers Programme.

  6. Effect of the Great Activity Programme on healthy lifestyle behaviours in 7-11 year olds.

    Science.gov (United States)

    Morris, John G; Gorely, Trish; Sedgwick, Matthew J; Nevill, Alan; Nevill, Mary E

    2013-01-01

    The study investigated the effect of a school-based healthy lifestyles intervention on physical activity and dietary variables. In total 378 children (177 intervention, 201 control; age 9.75 ± 0.82 years (mean ± s)) took part in the 7-month intervention comprising: preparation for and participation in 3 highlight events (a dance festival, a walking event and a running event); an interactive website for pupils, teachers and parents; and vacation activity planners. Primary outcome measures were objectively measured physical activity (pedometers and accelerometers), endurance fitness and dietary variables. Multi-level modelling was employed for data analysis. The increase in physical activity was greater in the intervention group than the control group (steps: 1049 vs 632 daily steps each month; moderate to vigorous physical activity (MVPA) total: 4.6 min · day(-1) · month(-1) vs 1.3 min · day(-1) · month(-1); MVPA bouts: 5.4 min · day(-1) · month(-1) vs 2.6 min · day(-1) · month(-1); all P fitness test distance was greater for intervention participants (46 vs 29 m · month(-1) of intervention, group × month interaction, P healthy lifestyles or psychological variables. Thus an intervention centred around highlight events and including relatively few additional resources can impact positively on the objectively measured physical activity of children.

  7. European Identity through Space Space Activities and Programmes as a Tool to Reinvigorate the European Identity

    CERN Document Server

    Baranes, Blandina

    2013-01-01

    Space activities are one particularly visible area, where Europe can shape its identity. This publication investigates the effect space activities have already had on building a European “spirit” (e.g. through European missions or European astronauts). It also looks into the effects that activities labelled “national” have on the emergence of a European identity. Based on this analysis, the book's intention is to identify creative ways and means for how to further use space for shaping the European identity. For this purpose the focus is not only on policy analysis but also on expertise from the fields of cultural science and the arts in order to tap their creative potential and also their theoretical approaches to the topic. Selecting this theme provides ESPI with another opportunity to develop its trans-disciplinary approach.

  8. Expression of preoperative KISS1 gene in tumor tissue with epithelial ovarian cancer and its prognostic value.

    Science.gov (United States)

    Cao, Fang; Chen, Liping; Liu, Manhua; Lin, Weiwei; Ji, Jinlong; You, Jun; Qiao, Fenghai; Liu, Hongbin

    2016-11-01

    Our study aimed to elucidate the role of Kisspeptin (KISS1) in tumor tissues of patients with epithelial ovarian cancer (EOC) and investigate the prognostic value of this biomarker.Forty EOC patients and 20 uterine fibroids female patients with healthy ovaries undergoing cytoreductive surgery between January 2010 and January 2014 in our hospital were enrolled in this study. KISS1 expression in tumor and normal tissues was detected. Correlations between clinic-pathologic variables and KISS1 expression in EOC tissues and the prognostic value of KISS1 for overall survival were evaluated.During the follow-up of 11.2 to 62.1 months, the overall survival rate and mean survival time were 28.9% (11/38) and 38.35 ± 2.84 months. Preoperative KISS1 mRNA was higher in tumor tissue than in normal tissue (P <0.001), and it was associated with histologic grade of tumor, surgical FIGO stage, metastasis, and residual tumor size (all P <0.05). Multivariate survival analysis indicated significant influence of residual tumor size (HR = 2.357, P = 0.039) and preoperative KISS1 mRNA (HR = 0.0001, P <0.001) on mean survival time. Patients with low KISS1 mRNA expression had shorter survival time than those with high expression (P = 0.001).Preoperative KISS1 mRNA was a potential prognostic biomarker for EOC, and high preoperative KISS1 expression indicated a favorable prognosis.

  9. Strategies to improve the implementation of healthy eating, physical activity and obesity prevention policies, practices or programmes within childcare services.

    Science.gov (United States)

    Wolfenden, Luke; Jones, Jannah; Williams, Christopher M; Finch, Meghan; Wyse, Rebecca J; Kingsland, Melanie; Tzelepis, Flora; Wiggers, John; Williams, Amanda J; Seward, Kirsty; Small, Tameka; Welch, Vivian; Booth, Debbie; Yoong, Sze Lin

    2016-10-04

    Despite the existence of effective interventions and best-practice guideline recommendations for childcare services to implement policies, practices and programmes to promote child healthy eating, physical activity and prevent unhealthy weight gain, many services fail to do so. The primary aim of the review was to examine the effectiveness of strategies aimed at improving the implementation of policies, practices or programmes by childcare services that promote child healthy eating, physical activity and/or obesity prevention. The secondary aims of the review were to:1. describe the impact of such strategies on childcare service staff knowledge, skills or attitudes;2. describe the cost or cost-effectiveness of such strategies;3. describe any adverse effects of such strategies on childcare services, service staff or children;4. examine the effect of such strategies on child diet, physical activity or weight status. We searched the following electronic databases on 3 August 2015: the Cochrane Central Register of Controlled trials (CENTRAL), MEDLINE, MEDLINE In Process, EMBASE, PsycINFO, ERIC, CINAHL and SCOPUS. We also searched reference lists of included trials, handsearched two international implementation science journals and searched the World Health Organization International Clinical Trials Registry Platform (www.who.int/ictrp/) and ClinicalTrials.gov (www.clinicaltrials.gov). We included any study (randomised or non-randomised) with a parallel control group that compared any strategy to improve the implementation of a healthy eating, physical activity or obesity prevention policy, practice or programme by staff of centre-based childcare services to no intervention, 'usual' practice or an alternative strategy. The review authors independently screened abstracts and titles, extracted trial data and assessed risk of bias in pairs; we resolved discrepancies via consensus. Heterogeneity across studies precluded pooling of data and undertaking quantitative

  10. Active Agents of Health Promotion? The School's Role in Supporting the HPV Vaccination Programme

    Science.gov (United States)

    Spratt, Jennifer; Shucksmith, Janet; Philip, Kate; McNaughton, Rebekah

    2013-01-01

    By providing a place in which children can be accessed, the school has long been a site for population-level health initiatives. Recent policy shifts towards health-promoting schools have however re-cast the school from passive host to active collaborator in public health. This paper examines secondary school teachers' views of their roles as…

  11. Positive Youth Development through an Outdoor Physical Activity Programme: Evidence from a Four-Year Evaluation

    Science.gov (United States)

    Armour, Kathleen; Sandford, Rachel

    2013-01-01

    In 2006, Sandford, Armour and Warmington undertook a comprehensive review of the literature on the role of physical activity/sport and physical education in promoting positive development for disaffected youth. This paper revisits the findings of the literature review in light of data from a four-year evaluation of one corporate-sponsored physical…

  12. Male Adolescents' View on Sexual Activity as Basis for the Development of Aids-Prevention Programmes.

    Science.gov (United States)

    Steyn, Hester; Myburgh, Chris P.H.; Poggenpoel, Marie

    2005-01-01

    The world regards AIDS as the most disastrous health threat in the world. HIV/AIDS- related issues amongst adolescents were initially neglected due to the visible impact of this pandemic on babies and adults. Adolescents' behavior is however regarded as high-risk because of their involvement in sexual activities from a young age. The purpose of…

  13. 亲吻,来自何时?%How did the custom of Kissing otart?

    Institute of Scientific and Technical Information of China (English)

    张梦华

    2003-01-01

    @@ Over time, the custom of kissing developed as a way for adults to express their love and affection for one another. The roots of this form of affection can be traced back to primitive times, when mothers fondled (爱抚)their children, much as mothers do today.

  14. Metastasis suppressor KISS1 seems to reverse the Warburg effect by enhancing mitochondrial biogenesis

    Science.gov (United States)

    Cancer cells tend to utilize aerobic glycolysis even under normoxic conditions, commonly called the "Warburg Effect." Aerobic glycolysis often directly correlates with malignancy, but its purpose, if any, in metastasis remains unclear. When wild-type KISS1 metastasis suppressor is expressed, aerob...

  15. KISS1 over-expression suppresses metastasis of pancreatic adenocarcinoma in a xenograft mouse model

    Science.gov (United States)

    Identifying molecular targets for treatment of pancreatic cancer metastasis is critical due to the high frequency of dissemination prior to diagnosis of this lethal disease. Because the KISS1 metastasis suppressor is expressed at reduced levels in advanced pancreatic cancer, we hypothesized that re-...

  16. Why heaven kissed earth : the christology of Thomas Goodwin (1600-1680)

    NARCIS (Netherlands)

    Jones, Mark

    2009-01-01

    My dissertation, “Why Heaven Kissed Earth”, looks at the Christology of the Reformed theologian and English Puritan, Thomas Goodwin (1600-1680). The thesis attempts to answer a question first posed by St. Anselm of Cantebury, namely, Cur Deus Homo (“Why did God become man?). Goodwin answers that

  17. THE KISSING NUMBER OF THE REGULAR PENTAGON%正五边形的吻合数

    Institute of Scientific and Technical Information of China (English)

    赵立宽

    2001-01-01

    设Pn是任意一个正n边形,k(Pn)表示Pn的吻合数,该文证明了k(P5)=6.%Let Pn be an arbitrary regular polygon containing n sides and k(Pn) its kissing number, then k(P5)=6 is obtained.

  18. The essential role of stacking adenines in a two-base-pair RNA kissing complex.

    Science.gov (United States)

    Stephenson, William; Asare-Okai, Papa Nii; Chen, Alan A; Keller, Sean; Santiago, Rachel; Tenenbaum, Scott A; Garcia, Angel E; Fabris, Daniele; Li, Pan T X

    2013-04-17

    In minimal RNA kissing complexes formed between hairpins with cognate GACG tetraloops, the two tertiary GC pairs are likely stabilized by the stacking of 5'-unpaired adenines at each end of the short helix. To test this hypothesis, we mutated the flanking adenines to various nucleosides and examined their effects on the kissing interaction. Electrospray ionization mass spectrometry was used to detect kissing dimers in a multiequilibria mixture, whereas optical tweezers were applied to monitor the (un)folding trajectories of single RNA molecules. The experimental findings were rationalized by molecular dynamics simulations. Together, the results showed that the stacked adenines are indispensable for the tertiary interaction. By shielding the tertiary base pairs from solvent and reducing their fraying, the stacked adenines made terminal pairs act more like interior base pairs. The purine double-ring of adenine was essential for effective stacking, whereas additional functional groups modulated the stabilizing effects through varying hydrophobic and electrostatic forces. Furthermore, formation of the kissing complex was dominated by base pairing, whereas its dissociation was significantly influenced by the flanking bases. Together, these findings indicate that unpaired flanking nucleotides play essential roles in the formation of otherwise unstable two-base-pair RNA tertiary interactions.

  19. Developmental programming of energy balance regulation: is physical activity more 'programmable' than food intake?

    Science.gov (United States)

    Zhu, Shaoyu; Eclarinal, Jesse; Baker, Maria S; Li, Ge; Waterland, Robert A

    2016-02-01

    Extensive human and animal model data show that environmental influences during critical periods of prenatal and early postnatal development can cause persistent alterations in energy balance regulation. Although a potentially important factor in the worldwide obesity epidemic, the fundamental mechanisms underlying such developmental programming of energy balance are poorly understood, limiting our ability to intervene. Most studies of developmental programming of energy balance have focused on persistent alterations in the regulation of energy intake; energy expenditure has been relatively underemphasised. In particular, very few studies have evaluated developmental programming of physical activity. The aim of this review is to summarise recent evidence that early environment may have a profound impact on establishment of individual propensity for physical activity. Recently, we characterised two different mouse models of developmental programming of obesity; one models fetal growth restriction followed by catch-up growth, and the other models early postnatal overnutrition. In both studies, we observed alterations in body-weight regulation that persisted to adulthood, but no group differences in food intake. Rather, in both cases, programming of energy balance appeared to be due to persistent alterations in energy expenditure and spontaneous physical activity (SPA). These effects were stronger in female offspring. We are currently exploring the hypothesis that developmental programming of SPA occurs via induced sex-specific alterations in epigenetic regulation in the hypothalamus and other regions of the central nervous system. We will summarise the current progress towards testing this hypothesis. Early environmental influences on establishment of physical activity are likely an important factor in developmental programming of energy balance. Understanding the fundamental underlying mechanisms in appropriate animal models will help determine whether early life

  20. Systematic review on the financial return of worksite health promotion programmes aimed at improving nutrition and/or increasing physical activity

    NARCIS (Netherlands)

    Dongen, J.M. van; Proper, K.I.; Wier, M.F. van; Beek, A.J. van der; Bongers, P.M.; Mechelen, W. van; Tulder, M.W. van

    2011-01-01

    Summary: This systematic review summarizes the current evidence on the financial return of worksite health promotion programmes aimed at improving nutrition and/or increasing physical activity. Data on study characteristics and results were extracted from 18 studies published up to 14 January 2011.

  1. Cost-effectiveness of a long-term Internet-delivered worksite health promotion programme on physical activity and nutrition: A cluster randomized controlled trial

    NARCIS (Netherlands)

    S.J.W. Robroek (Suzan); S. Polinder (Suzanne); F.J. Bredt (Folef); A. Burdorf (Alex)

    2012-01-01

    textabstractThis study aims to evaluate the cost-effectiveness of a long-term workplace health promotion programme on physical activity (PA) and nutrition. In total, 924 participants enrolled in a 2-year cluster randomized controlled trial, with departments (n = 74) within companies (n = 6) as the u

  2. Design of a process evaluation of the implementation of a physical activity and sports stimulation programme in Dutch rehabilitation setting : ReSpAct

    NARCIS (Netherlands)

    Hoekstra, Femke; Alingh, Roelina A.; van der Schans, Cees P.; Hettinga, Florentina J.; Duijf, Marjo; Dekker, Rienk; van der Woude, Lucas H. V.

    2014-01-01

    Background: There is a growing interest to study the transfer of evidence-based information into daily practice. The evidence-based programme Rehabilitation, Sports and Exercise (RSE) that aims to stimulate an active lifestyle during and after a rehabilitation period in people with a disability and/

  3. Melt With This Kiss: Paralyzing and Liquefying Venom of The Assassin Bug Pristhesancus plagipennis (Hemiptera: Reduviidae).

    Science.gov (United States)

    Walker, Andrew A; Madio, Bruno; Jin, Jiayi; Undheim, Eivind A B; Fry, Bryan G; King, Glenn F

    2017-04-01

    Assassin bugs (Hemiptera: Heteroptera: Reduviidae) are venomous insects, most of which prey on invertebrates. Assassin bug venom has features in common with venoms from other animals, such as paralyzing and lethal activity when injected, and a molecular composition that includes disulfide-rich peptide neurotoxins. Uniquely, this venom also has strong liquefying activity that has been hypothesized to facilitate feeding through the narrow channel of the proboscis-a structure inherited from sap- and phloem-feeding phytophagous hemipterans and adapted during the evolution of Heteroptera into a fang and feeding structure. However, further understanding of the function of assassin bug venom is impeded by the lack of proteomic studies detailing its molecular composition.By using a combined transcriptomic/proteomic approach, we show that the venom proteome of the harpactorine assassin bug Pristhesancus plagipennis includes a complex suite of >100 proteins comprising disulfide-rich peptides, CUB domain proteins, cystatins, putative cytolytic toxins, triabin-like protein, odorant-binding protein, S1 proteases, catabolic enzymes, putative nutrient-binding proteins, plus eight families of proteins without homology to characterized proteins. S1 proteases, CUB domain proteins, putative cytolytic toxins, and other novel proteins in the 10-16-kDa mass range, were the most abundant venom components. Thus, in addition to putative neurotoxins, assassin bug venom includes a high proportion of enzymatic and cytolytic venom components likely to be well suited to tissue liquefaction. Our results also provide insight into the trophic switch to blood-feeding by the kissing bugs (Reduviidae: Triatominae). Although some protein families such as triabins occur in the venoms of both predaceous and blood-feeding reduviids, the composition of venoms produced by these two groups is revealed to differ markedly. These results provide insights into the venom evolution in the insect suborder

  4. Origami-inspired active graphene-based paper for programmable instant self-folding walking devices.

    Science.gov (United States)

    Mu, Jiuke; Hou, Chengyi; Wang, Hongzhi; Li, Yaogang; Zhang, Qinghong; Zhu, Meifang

    2015-11-01

    Origami-inspired active graphene-based paper with programmed gradients in vertical and lateral directions is developed to address many of the limitations of polymer active materials including slow response and violent operation methods. Specifically, we used function-designed graphene oxide as nanoscale building blocks to fabricate an all-graphene self-folding paper that has a single-component gradient structure. A functional device composed of this graphene paper can (i) adopt predesigned shapes, (ii) walk, and (iii) turn a corner. These processes can be remote-controlled by gentle light or heating. We believe that this self-folding material holds potential for a wide range of applications such as sensing, artificial muscles, and robotics.

  5. Bio-optofluidics and Bio-photonics: Programmable Phase Optics activities at DTU Fotonik

    DEFF Research Database (Denmark)

    Bañas, Andrew Rafael; Palima, Darwin; Pedersen, Finn

    We present ongoing research and development activities for constructing a compact next generation BioPhotonics Workstation and a Bio-optofluidic Cell Sorter (cell-BOCS) for all-optical micromanipulation platforms utilizing low numerical aperture beam geometries. Unlike conventional high NA optica...... the BioPhotonics Workstation platform more photon efficient by studying the 3D distribution of the counter propagating beams and utilizing the Generalized Phase Contrast (GPC) method for illuminating the applied spatial light modulators.......We present ongoing research and development activities for constructing a compact next generation BioPhotonics Workstation and a Bio-optofluidic Cell Sorter (cell-BOCS) for all-optical micromanipulation platforms utilizing low numerical aperture beam geometries. Unlike conventional high NA optical...

  6. Origami-inspired active graphene-based paper for programmable instant self-folding walking devices

    Science.gov (United States)

    Mu, Jiuke; Hou, Chengyi; Wang, Hongzhi; Li, Yaogang; Zhang, Qinghong; Zhu, Meifang

    2015-01-01

    Origami-inspired active graphene-based paper with programmed gradients in vertical and lateral directions is developed to address many of the limitations of polymer active materials including slow response and violent operation methods. Specifically, we used function-designed graphene oxide as nanoscale building blocks to fabricate an all-graphene self-folding paper that has a single-component gradient structure. A functional device composed of this graphene paper can (i) adopt predesigned shapes, (ii) walk, and (iii) turn a corner. These processes can be remote-controlled by gentle light or heating. We believe that this self-folding material holds potential for a wide range of applications such as sensing, artificial muscles, and robotics. PMID:26601135

  7. Effect of androgen on Kiss1 expression and luteinizing hormone release in female rats.

    Science.gov (United States)

    Iwata, Kinuyo; Kunimura, Yuyu; Matsumoto, Keisuke; Ozawa, Hitoshi

    2017-06-01

    Hyperandrogenic women have various grades of ovulatory dysfunction, which lead to infertility. The purpose of this study was to determine whether chronic exposure to androgen affects the expression of kisspeptin (ovulation and follicle development regulator) or release of luteinizing hormone (LH) in female rats. Weaned females were subcutaneously implanted with 90-day continuous-release pellets of 5α-dihydrotestosterone (DHT) and studied after 10 weeks of age. Number of Kiss1-expressing cells in both the anteroventral periventricular nucleus (AVPV) and arcuate nucleus (ARC) was significantly decreased in ovary-intact DHT rats. Further, an estradiol-induced LH surge was not detected in DHT rats, even though significant differences were not observed between DHT and non-DHT rats with regard to number of AVPV Kiss1-expressing cells or gonadotrophin-releasing hormone (GnRH)-immunoreactive (ir) cells in the presence of high estradiol. Kiss1-expressing and neurokinin B-ir cells were significantly decreased in the ARC of ovariectomized (OVX) DHT rats compared with OVX non-DHT rats; pulsatile LH secretion was also suppressed in these animals. Central injection of kisspeptin-10 or intravenous injection of a GnRH agonist did not affect the LH release in DHT rats. Notably, ARC Kiss1-expressing cells expressed androgen receptors (ARs) in female rats, whereas only a few Kiss1-expressing cells expressed ARs in the AVPV. Collectively, our results suggest excessive androgen suppresses LH surge and pulsatile LH secretion by inhibiting kisspeptin expression in the ARC and disruption at the pituitary level, whereas AVPV kisspeptin neurons appear to be directly unaffected by androgen. Hence, hyperandrogenemia may adversely affect ARC kisspeptin neurons, resulting in anovulation and menstrual irregularities. © 2017 Society for Endocrinology.

  8. Kissing inflation is feasible with all second-generation drug-eluting balloons.

    Science.gov (United States)

    Sgueglia, Gregory A; Todaro, Daniel; Bisciglia, Andrea; Conte, Micaela; Stipo, Antonino; Pucci, Edoardo

    2011-01-01

    To assess the feasibility of kissing second-generation drug-eluting balloons (DEB), which have better mechanical properties than the first-generation DEB, in order to optimize provisional bare-metal stenting (BMS) when treating coronary bifurcation lesions in patients with contraindication to drug-eluting stents. Consecutive patients with anticipated low compliance to dual antiplatelet therapy who are undergoing provisional stenting with an open-cell design BMS and final kissing balloon with second-generation DEB were enrolled in this feasibility study. Angiographic success and procedural success (i.e., angiographic success in absence of in hospital major cardiovascular events) were registered. Clinical follow-up was also attempted in all patients. A total of 14 patients (mean age 66±9 years, nine men) participated on the study. The DEB used were SeQuent Please (B. Braun Melsungen, Berlin, Germany) in six patients, In.Pact Falcon (Medtronic Invatec, Roncadelle, Italy) in four patients, New Dior (Eurocor, Bonn, Germany) in two patients and Pantera Lux (Biotronik, Berlin, Germany) in another two patients. All procedures, but one, were performed by transradial access through a 6-French high-flow guiding catheter. True bifurcation was present in 50% of the patients. Angiographic and procedural success was obtained in all patients. At a mean follow-up of 234±81 days, all contacted patients were asymptomatic and free from major adverse cardiac events (including cardiac death, nonfatal myocardial infarction and target bifurcation revascularization). At the advent of dedicated bifurcation stents, kissing DEB appears safe and effective and can be used to implement innovative, simpler, safer and possibly more effective bifurcation techniques. These remarkable results have laid the ground for an ongoing prospective registry of the kissing DEB technique (KISSING DEBBIE study, ClinicalTrials.gov NCT01009996). Copyright © 2011 Elsevier Inc. All rights reserved.

  9. Bio-optofluidics and Bio-photonics: Programmable Phase Optics activities at DTU Fotonik

    DEFF Research Database (Denmark)

    Bañas, Andrew Rafael; Palima, Darwin; Pedersen, Finn

    We present ongoing research and development activities for constructing a compact next generation BioPhotonics Workstation and a Bio-optofluidic Cell Sorter (cell-BOCS) for all-optical micromanipulation platforms utilizing low numerical aperture beam geometries. Unlike conventional high NA optical...... tweezers, the BioPhotonics workstation is e.g. capable of long range 3D manipulation. This enables a variety of biological studies such as manipulation of intricate microfabricated assemblies or for automated and parallel optofluidic cell sorting. To further reduce its overhead, we propose ways of making...

  10. Programmable SERS active substrates for chemical and biosensing applications using amorphous/crystalline hybrid silicon nanomaterial

    Science.gov (United States)

    Powell, Jeffery Alexander; Venkatakrishnan, Krishnan; Tan, Bo

    2016-01-01

    We present the creation of a unique nanostructured amorphous/crystalline hybrid silicon material that exhibits surface enhanced Raman scattering (SERS) activity. This nanomaterial is an interconnected network of amorphous/crystalline nanospheroids which form a nanoweb structure; to our knowledge this material has not been previously observed nor has it been applied for use as a SERS sensing material. This material is formed using a femtosecond synthesis technique which facilitates a laser plume ion condensation formation mechanism. By fine-tuning the laser plume temperature and ion interaction mechanisms within the plume, we are able to precisely program the relative proportion of crystalline Si to amorphous Si content in the nanospheroids as well as the size distribution of individual nanospheroids and the size of Raman hotspot nanogaps. With the use of Rhodamine 6G (R6G) and Crystal Violet (CV) chemical dyes, we have been able to observe a maximum enhancement factor of 5.38 × 106 and 3.72 × 106 respectively, for the hybrid nanomaterial compared to a bulk Si wafer substrate. With the creation of a silicon-based nanomaterial capable of SERS detection of analytes, this work demonstrates a redefinition of the role of nanostructured Si from an inactive to SERS active role in nano-Raman sensing applications.

  11. The testosterone-dependent and independent transcriptional networks in the hypothalamus of Gpr54 and Kiss1 knockout male mice are not fully equivalent

    Directory of Open Access Journals (Sweden)

    Sutcliffe Margaret

    2011-04-01

    Full Text Available Abstract Background Humans and mice with loss of function mutations in GPR54 (KISS1R or kisspeptin do not progress through puberty, caused by a failure to release GnRH. The transcriptional networks regulated by these proteins in the hypothalamus have yet to be explored by genome-wide methods. Results We show here, using 1 million exon mouse arrays (Exon 1.0 Affymetrix and quantitative polymerase chain reaction (QPCR validation to analyse microdissected hypothalamic tissue from Gpr54 and Kiss1 knockout mice, the extent of transcriptional regulation in the hypothalamus. The sensitivity to detect important transcript differences in microdissected RNA was confirmed by the observation of counter-regulation of Kiss1 expression in Gpr54 knockouts and confirmed by immunohistochemistry (IHC. Since Gpr54 and Kiss1 knockout animals are effectively pre-pubertal with low testosterone (T levels, we also determined which of the validated transcripts were T-responsive and which varied according to genotype alone. We observed four types of transcriptional regulation (i genotype only dependent regulation, (ii T only dependent regulation, (iii genotype and T-dependent regulation with interaction between these variables, (iv genotype and T-dependent regulation with no interaction between these variables. The results implicate for the first time several transcription factors (e.g. Npas4, Esr2, proteases (Klk1b22, and the orphan 10-transmembrane transporter TMEM144 in the biology of GPR54/kisspeptin function in the hypothalamus. We show for the neuronal activity regulated transcription factor NPAS4, that distinct protein over-expression is seen in the hypothalamus and hippocampus in Gpr54 knockout mice. This links for the first time the hypothalamic-gonadal axis with this important regulator of inhibitory synapse formation. Similarly we confirm TMEM144 up-regulation in the hypothalamus by RNA in situ hybridization and western blot. Conclusions Taken together, global

  12. The testosterone-dependent and independent transcriptional networks in the hypothalamus of Gpr54 and Kiss1 knockout male mice are not fully equivalent

    Science.gov (United States)

    2011-01-01

    Background Humans and mice with loss of function mutations in GPR54 (KISS1R) or kisspeptin do not progress through puberty, caused by a failure to release GnRH. The transcriptional networks regulated by these proteins in the hypothalamus have yet to be explored by genome-wide methods. Results We show here, using 1 million exon mouse arrays (Exon 1.0 Affymetrix) and quantitative polymerase chain reaction (QPCR) validation to analyse microdissected hypothalamic tissue from Gpr54 and Kiss1 knockout mice, the extent of transcriptional regulation in the hypothalamus. The sensitivity to detect important transcript differences in microdissected RNA was confirmed by the observation of counter-regulation of Kiss1 expression in Gpr54 knockouts and confirmed by immunohistochemistry (IHC). Since Gpr54 and Kiss1 knockout animals are effectively pre-pubertal with low testosterone (T) levels, we also determined which of the validated transcripts were T-responsive and which varied according to genotype alone. We observed four types of transcriptional regulation (i) genotype only dependent regulation, (ii) T only dependent regulation, (iii) genotype and T-dependent regulation with interaction between these variables, (iv) genotype and T-dependent regulation with no interaction between these variables. The results implicate for the first time several transcription factors (e.g. Npas4, Esr2), proteases (Klk1b22), and the orphan 10-transmembrane transporter TMEM144 in the biology of GPR54/kisspeptin function in the hypothalamus. We show for the neuronal activity regulated transcription factor NPAS4, that distinct protein over-expression is seen in the hypothalamus and hippocampus in Gpr54 knockout mice. This links for the first time the hypothalamic-gonadal axis with this important regulator of inhibitory synapse formation. Similarly we confirm TMEM144 up-regulation in the hypothalamus by RNA in situ hybridization and western blot. Conclusions Taken together, global transcriptional

  13. A ‘Timed’ Kiss is Essential for Reproduction: Lessons from Mammalian Studies

    Directory of Open Access Journals (Sweden)

    Manish Putteeraj

    2016-08-01

    Full Text Available Reproduction is associated with the circadian system, primarily as a result of the connectivity between the biological clock in the suprachiasmatic nucleus (SCN and reproduction-regulating brain regions, such as preoptic area (POA, anteroventral periventricular nucleus (AVPV and arcuate nucleus (ARC. Networking of the central pacemaker to these hypothalamic brain regions is partly represented by close fiber appositions to specialised neurons, such as kisspeptin and gonadotropin-releasing hormone (GnRH neurons; accounting for rhythmic release of gonadotropins and sex steroids. Numerous studies have attempted to dissect the neurochemical properties of GnRH neurons, which possess intrinsic oscillatory features through the presence of clock genes to regulate the pulsatile and circadian secretion. However, less attention has been given to kisspeptin, the upstream regulator of GnRH and a potent mediator of reproductive functions including puberty. Kisspeptin exerts its stimulatory effects on GnRH secretion via its cognate Kiss-1R receptor that is co-expressed on GnRH neurons. Emerging studies have found that kisspeptin neurons oscillate on a circadian basis and that these neurons also express clock genes that are thought to regulate its rhythmic activities. Based on the fiber networks between the SCN and reproductive nuclei such as the POA, AVPV, ARC, it is suggested that interactions among the central biological clock and reproductive neurons ensure optimal reproductive functionality. Within this neuronal circuitry, kisspeptin neuronal system is likely to ‘time’ reproduction in a long term during development and ageing, in a medium term to regulate circadian or estrus cycle, and in a short term to regulate pulsatile GnRH secretion.

  14. Erythroid cell mitochondria receive endosomal iron by a "kiss-and-run" mechanism.

    Science.gov (United States)

    Hamdi, Amel; Roshan, Tariq M; Kahawita, Tanya M; Mason, Anne B; Sheftel, Alex D; Ponka, Prem

    2016-12-01

    In erythroid cells, more than 90% of transferrin-derived iron enters mitochondria where ferrochelatase inserts Fe(2+) into protoporphyrin IX. However, the path of iron from endosomes to mitochondrial ferrochelatase remains elusive. The prevailing opinion is that, after its export from endosomes, the redox-active metal spreads into the cytosol and mysteriously finds its way into mitochondria through passive diffusion. In contrast, this study supports the hypothesis that the highly efficient transport of iron toward ferrochelatase in erythroid cells requires a direct interaction between transferrin-endosomes and mitochondria (the "kiss-and-run" hypothesis). Using a novel method (flow sub-cytometry), we analyze lysates of reticulocytes after labeling these organelles with different fluorophores. We have identified a double-labeled population definitively representing endosomes interacting with mitochondria, as demonstrated by confocal microscopy. Moreover, we conclude that this endosome-mitochondrion association is reversible, since a "chase" with unlabeled holotransferrin causes a time-dependent decrease in the size of the double-labeled population. Importantly, the dissociation of endosomes from mitochondria does not occur in the absence of holotransferrin. Additionally, mutated recombinant holotransferrin, that cannot release iron, significantly decreases the uptake of (59)Fe by reticulocytes and diminishes (59)Fe incorporation into heme. This suggests that endosomes, which are unable to provide iron to mitochondria, cause a "traffic jam" leading to decreased endocytosis of holotransferrin. Altogether, our results suggest that a molecular mechanism exists to coordinate the iron status of endosomal transferrin with its trafficking. Besides its contribution to the field of iron metabolism, this study provides evidence for a new intracellular trafficking pathway of organelles. Copyright © 2016 Elsevier B.V. All rights reserved.

  15. Mass-casualty Response to the Kiss Nightclub in Santa Maria, Brazil.

    Science.gov (United States)

    Dal Ponte, Silvana T; Dornelles, Carlos F D; Arquilla, Bonnie; Bloem, Christina; Roblin, Patricia

    2015-02-01

    On January 27, 2013, a fire at the Kiss Nightclub in Santa Maria, Brazil led to a mass-casualty incident affecting hundreds of college students. A total of 234 people died on scene, 145 were hospitalized, and another 623 people received treatment throughout the first week following the incident.1 Eight of the hospitalized people later died.1 The Military Police were the first on scene, followed by the state fire department, and then the municipal Mobile Prehospital Assistance (SAMU) ambulances. The number of victims was not communicated clearly to the various units arriving on scene, leading to insufficient rescue personnel and equipment. Incident command was established on scene, but the rescuers and police were still unable to control the chaos of multiple bystanders attempting to assist in the rescue efforts. The Municipal Sports Center (CDM) was designated as the location for dead bodies, where victim identification and communication with families occurred, as well as forensic evaluation, which determined the primary cause of death to be asphyxia. A command center was established at the Hospital de Caridade Astrogildo de Azevedo (HCAA) in Santa Maria to direct where patients should be admitted, recruit staff, and procure additional supplies, as needed. The victims suffered primarily from smoke inhalation and many required endotracheal intubation and mechanical ventilation. There was a shortage of ventilators; therefore, some had to be borrowed from local hospitals, neighboring cities, and distant areas in the state. A total of 54 patients1 were transferred to hospitals in the capital city of Porto Alegre (Brazil). The main issues with the response to the fire were scene control and communication. Areas for improvement were identified, namely the establishment of a disaster-response plan, as well as regularly scheduled training in disaster preparedness/response. These activities are the first steps to improving mass-casualty responses.

  16. Nutritional counselling and its effects on diet, nutritional knowledge and status, physical activity and quality of life in a Southern Europe population: evaluation of a health promotion programme.

    Directory of Open Access Journals (Sweden)

    Cecilia Quercioli

    2011-06-01

    Full Text Available

    Abstract:
    Background: Health promotion and primary prevention are key points for fighting the increase in non- communicable diseases. Aim: To test the effectiveness of a nutritional counselling programme in improving nutritional behaviours, knowledge and status, physical activity and health-related quality of life in a general population. Methods: In the period between January–December 2007, we studied the application of the nutritional counselling programme (NCP developed by Local Health Agency 10 (Florence-Italy. We compared , diet, nutritional knowledge, physical activity, health related quality of life (measured by SF36 questionnaire, body mass index (BMI and waist circumference before and after a two months intensive nutritional counselling programme using the paired t test and McNemar test. Diet, nutritional knowledge, physical activity, socio-demographic and morbidity information were collected through questionnaires. BMI and waist circumference were assessed by a medical doctor. Results: We enrolled 74 persons, 59 of whom completed the educational programme. Of these, 34 had a nutritional status assessment after the programme. Mean age was 49 years, 80% were females. BMI, waist circumference and diet, except for water intake, did not change. The percentage of people who had “never exercised in a week" decreased from 46% to 17% (p<0.001. Mean percentage of right answers to nutritional knowledge indicators increased from 64% to 78% (p<0.001. Health related quality of life (HRQL improved especially with regard to “Physical" and “Emotional Role". Conclusions: The NCP showed important results in promoting physical activity and improving HRQL and nutritional knowledge, moderate/none results in improving diet and nutritional status.

  17. Evaluation of beta spectrometry for environmental monitoring of undeclared nuclear activities. Report on task FIN A846 on the Finnish support programme to IAEA safeguards

    Energy Technology Data Exchange (ETDEWEB)

    Rosenberg, R.; Lipponen, M. [VTT Chemical Technology, Espoo (Finland); Jaakkola, T. [Helsinki Univ. (Finland). Dept. of Radiochemistry

    1996-03-01

    The IAEA (the Agency) is considering environmental monitoring as a possible method to detect the presence of undeclared nuclear activities in a country covered by a comprehensive safeguards agreement. The Finnish support program (FINSP), implemented by the Finnish Centre for Radiation and Nuclear Safety (STUK), is supporting the Agency in this new programme. This Agency Task FIN A 846, addresses the possibility of using beta-emitting nuclides as indicators for covert nuclear fuel reprocessing activities. (90 refs., 1 fig., 12 tabs.).

  18. The SEDIBUD (Sediment Budgets in Cold Environments) Programme: Ongoing activities and selected key tasks for the coming years

    Science.gov (United States)

    Beylich, Achim A.; Lamoureux, Scott F.; Decaulne, Armelle

    2012-09-01

    Projected climate change in cold environments is expected to alter melt-season duration and intensity, along with the number of extreme rainfall events, total annual precipitation and the balance between snowfall and rainfall. In addition, changes to the thermal balance are expected to reduce the extent of permafrost and seasonal ground frost and increase active layer depths. The combined effects of these changes will alter surface environments in cold climate regions and change the fluxes of sediments, nutrients and solutes, but the absence of data, coordinated process monitoring and coordinated quantitative analysis to understand the sensitivity of the Earth surface environment are acute in cold climate environments. The International Association of Geomorphologists (I.A.G./A.I.G.) SEDIBUD (Sediment Budgets in Cold Environments) Programme has been formed to address this key knowledge gap and builds on the earlier European Science Foundation (ESF) SEDIFLUX (Sedimentary Source-to-Sink-Fluxes in Cold Environments) Network. Coordinated efforts are carried out to monitor, quantify, compare and model sedimentary fluxes and possible effects of predicted climate change in currently 44 selected SEDIBUD Key Test Sites (cold climate environment catchments) worldwide.

  19. Activating welfare recipients with health complaints: Reasons for failure of a cognitive training programme

    Directory of Open Access Journals (Sweden)

    Inge Bramsen

    2011-03-01

    Een groeiende nadruk op de activering van uitkeringsgerechtigden heeft in veel Westerse verzorgingsstaten geleid tot de ontwikkeling van interventies die gericht zijn op uitkeringsgerechtigden met gezondheidsklachten. Vormen van dwang, zoals verplichte participatie, worden meer en meer toegepast binnen dit activeringsbeleid. Een voorbeeld hiervan is het Nederlandse project “Gezond aan de Slag”, dat een interventie ontwikkelde en evalueerde waarin een fysiek trainingsprogramma gecombineerd wordt met cognitieve training. Hoewel HtW als “best practice” gold, liet een randomized trial geen positieve effecten van de interventie zien. Omdat het cognitieve trainingsprogramma, gebaseerd op rationeel-emotieve therapie, niet eerder afzonderlijk werd bestudeerd, werd een diepgaande kwalitatieve studie uitgevoerd. Getracht werd om de oorzaken van het falen van het programma in kaart te brengen. Zes, gericht geselecteerde, trainers werden geïnterviewd. De resultaten lieten zien dat de trainers problemen hadden met de heterogeniteit van de deelnemersgroep en op andere problemen, verwachtingen en arbeidsmarktobstakels stuitten dan vooraf verwacht. De verplichte aard van HtW had een negatief effect op de motivatie van zowel deelnemers als trainers. In de discussieparagraaf van dit artikel worden vraagtekens geplaatst bij de veronderstelling dat de coping stijl en cognities van uitkeringsgerechtigden met gezondheidsproblemen de belangrijkste hindernissen zijn voor het vinden van werk, en worden vraagtekens geplaatst bij het beleid om fysieke beperkingen van deelnemers te negeren. De auteurs suggereren dat met betrekking tot dit complexe praktijkprobleem, verbetering kan worden bereikt door aanpassen en leren van de interventie gedurende de toepassing daarvan toe te staan, en door expliciet te reflecteren op de normatieve aspecten van de interventie, en de perspectieven van de uitkeringsgerechtigden zelf.

  20. Mutations of the kissing-loop dimerization sequence influence the site specificity of murine leukemia virus recombination in vivo

    DEFF Research Database (Denmark)

    Mikkelsen, J G; Lund, Anders Henrik; Duch, M

    2000-01-01

    synthesis in newly infected cells. We have previously shown that template shifts within the 5' leader of murine leukemia viruses occur preferentially within the kissing stem-loop motif, a cis element crucial for in vitro RNA dimer formation. By use of a forced recombination approach based on single......-cycle transfer of Akv murine leukemia virus-based vectors harboring defective primer binding site sequences, we now report that modifications of the kissing-loop structure, ranging from a deletion of the entire sequence to introduction of a single point mutation in the loop motif, significantly disturb site...... specificity of recombination within the highly structured 5' leader region. In addition, we find that an intact kissing-loop sequence favors optimal RNA encapsidation and vector transduction. Our data are consistent with the kissing-loop dimerization model and suggest that a direct intermolecular RNA...

  1. Comparative analysis of kisspeptin-immunoreactivity reveals genuine differences in the hypothalamic Kiss1 systems between rats and mice

    DEFF Research Database (Denmark)

    Overgaard, Agnete; Tena-Sempere, Manuel; Franceschini, Isabelle

    2013-01-01

    Kiss1 mRNA and its corresponding peptide products, kisspeptins, are expressed in two restricted brain areas of rodents, the anteroventral periventricular nucleus (AVPV) and the arcuate nucleus (ARC). The concentration of mature kisspeptins may not directly correlate with Kiss1 mRNA levels, because...... mRNA translation and/or posttranslational modification, degradation, transportation and release of kisspeptins could be regulated independently of gene expression, and there may thus be differences in kisspeptin expression even in species with similar Kiss1 mRNA profiles. We measured and compared...... kisspeptin-immunoreactivity in both nuclei and both sexes of rats and mice and quantified kisspeptin-immunoreactive nerve fibers. We also determined Kiss1 mRNA levels and measured kisspeptin-immunoreactivity in colchicine pretreated rats. Overall, we find higher levels of kisspeptin...

  2. VIP in construction: systematic development and evaluation of a multifaceted health programme aiming to improve physical activity levels and dietary patterns among construction workers

    Directory of Open Access Journals (Sweden)

    Viester Laura

    2012-01-01

    Full Text Available Abstract Background The prevalence of both overweight and musculoskeletal disorders (MSD in the construction industry is high. Many interventions in the occupational setting aim at the prevention and reduction of these health problems, but it is still unclear how these programmes should be designed. To determine the effectiveness of interventions on these health outcomes randomised controlled trials (RCTs are needed. The aim of this study is to systematically develop a tailored intervention for prevention and reduction of overweight and MSD among construction workers and to describe the evaluation study regarding its (cost-effectiveness. Methods/Design The Intervention Mapping (IM protocol was applied to develop and implement a tailored programme aimed at the prevention and reduction of overweight and MSD. The (cost- effectiveness of the intervention programme will be evaluated using an RCT. Furthermore, a process evaluation will be conducted. The research population will consist of blue collar workers of a large construction company in the Netherlands. Intervention The intervention programme will be aimed at improving (vigorous physical activity levels and healthy dietary behaviour and will consist of tailored information, face-to-face and telephone counselling, training instruction (a fitness "card" to be used for exercises, and materials designed for the intervention (overview of the company health promoting facilities, waist circumference measuring tape, pedometer, BMI card, calorie guide, recipes, and knowledge test. Main study parameters/endpoints The intervention effect on body weight and waist circumference (primary outcome measures, as well as on lifestyle behaviour, MSD, fitness, CVD risk indicators, and work-related outcomes (i.e. productivity, sick leave (secondary outcome measures will be assessed. Discussion The development of the VIP in construction intervention led to a health programme tailored to the needs of construction

  3. Feasibility of an after-school group-based exercise and lifestyle programme to improve cardiorespiratory fitness and health in less-active Pacific and Maori adolescents.

    Science.gov (United States)

    Chansavang, Yongchie; Elley, C Raina; McCaffrey, Brighid; Davidson, Chloe; Dewes, Ofa; Dalleck, Lance

    2015-03-01

    Obesity and low levels of physical activity are increasing among Pacific and Maori adolescents in New Zealand. To assess the feasibility of an after-school exercise and lifestyle programme to improve cardiorespiratory fitness, health and usual activity in less-active Pacific and Maori adolescents over six weeks. Eighteen less-active secondary school students participated. The six-week programme included 3 x 1.5 hour exercise and healthy lifestyle sessions per week. Outcomes included estimated cardiorespiratory fitness (VO2max), insulin resistance (Homeostasis Model Assessment), physical activity, glycated haemoglobin (HbA1c), fasting plasma glucose, blood pressure, waist circumference and fasting lipids, measured at baseline and six weeks. Programme attendance and qualitative comments were also recorded. Student's t-tests were used. Of the 18 students enrolled, 16 (89%) completed six-week follow-up, 14 (78%) were female, 13 (72%) were Pacific ethnicity and 5 (28%) were Maori . At baseline, mean age was 16.3 (standard deviation [SD] 1.0) years, body mass index (BMI) 35.2 (SD 6.7) kg/m2, VO2max 31.5 (SD 4.3) mL/kg/min, systolic blood pressure 125.0 (SD 12.9) mm Hg, HbA1c 39.9 (SD 3.8) mmol/mol, fasting serum insulin 28.3 (SD 27.8) μU/mL. At follow-up, improvements had occurred in VO2max (3.2 mL/kg/min; p=0.02), systolic blood pressure (-10.6 mm Hg; p=0.003), HbA1c (-1.1 mmol/mol; p=0.03) and weekly vigorous (4 hours, p=0.002) and moderate (2 hours, p=0.006) physical activity, although waist circumference increased (p=0.005). Programme attendance was over 50%. Comments were mostly positive. The after-school exercise and lifestyle programme and study methods were feasible. Such programmes have the potential to improve health outcomes for Pacific and Maori adolescents.

  4. A combinatorial approach to the repertoire of RNA kissing motifs; towards multiplex detection by switching hairpin aptamers.

    Science.gov (United States)

    Durand, Guillaume; Dausse, Eric; Goux, Emma; Fiore, Emmanuelle; Peyrin, Eric; Ravelet, Corinne; Toulmé, Jean-Jacques

    2016-05-19

    Loop-loop (also known as kissing) interactions between RNA hairpins are involved in several mechanisms in both prokaryotes and eukaryotes such as the regulation of the plasmid copy number or the dimerization of retroviral genomes. The stability of kissing complexes relies on loop parameters (base composition, sequence and size) and base combination at the loop-loop helix - stem junctions. In order to identify kissing partners that could be used as regulatory elements or building blocks of RNA scaffolds, we analysed a pool of 5.2 × 10(6) RNA hairpins with randomized loops. We identified more than 50 pairs of kissing RNA hairpins. Two kissing motifs, 5'CCNY and 5'RYRY, generate highly stable complexes with KDs in the low nanomolar range. Such motifs were introduced in the apical loop of hairpin aptamers that switch between unfolded and folded state upon binding to their cognate target molecule, hence their name aptaswitch. The aptaswitch-ligand complex is specifically recognized by a second RNA hairpin named aptakiss through loop-loop interaction. Taking advantage of our kissing motif repertoire we engineered aptaswitch-aptakiss modules for purine derivatives, namely adenosine, GTP and theophylline and demonstrated that these molecules can be specifically and simultaneously detected by surface plasmon resonance or by fluorescence anisotropy. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

  5. SET-Routes programme

    CERN Multimedia

    Marietta Schupp, EMBL Photolab

    2008-01-01

    Dr Sabine Hentze, specialist in human genetics, giving an Insight Lecture entitled "Human Genetics – Diagnostics, Indications and Ethical Issues" on 23 September 2008 at EMBL Heidelberg. Activities in a achool in Budapest during a visit of Angela Bekesi, Ambassadors for the SET-Routes programme.

  6. Using intervention mapping to develop a culturally appropriate intervention to prevent childhood obesity: the HAPPY (Healthy and Active Parenting Programme for Early Years) study.

    Science.gov (United States)

    Taylor, Natalie J; Sahota, Pinki; Sargent, Judith; Barber, Sally; Loach, Jackie; Louch, Gemma; Wright, John

    2013-12-28

    Interventions that make extensive use of theory tend to have larger effects on behaviour. The Intervention Mapping (IM) framework incorporates theory into intervention design, implementation and evaluation, and was applied to the development of a community-based childhood obesity prevention intervention for a multi-ethnic population. IM was applied as follows: 1) Needs assessment of the community and culture; consideration of evidence-base, policy and practice; 2) Identification of desired outcomes and change objectives following identification of barriers to behaviour change mapped alongside psychological determinants (e.g. knowledge, self-efficacy, intention); 3) Selection of theory-based methods and practical applications to address barriers to behaviour change (e.g., strategies for responsive feeding); 4) Design of the intervention by developing evidence-based interactive activities and resources (e.g., visual aids to show babies stomach size). The activities were integrated into an existing parenting programme; 5) Adoption and implementation: parenting practitioners were trained by healthcare professionals to deliver the programme within Children Centres. HAPPY (Healthy and Active Parenting Programme for Early Years) is aimed at overweight and obese pregnant women (BMI > 25); consists of 12 × 2.5 hr. sessions (6 ante-natal from 24 weeks; 6 postnatal up to 9 months); it addresses mother's diet and physical activity, breast or bottle feeding, infant diet and parental feeding practices, and infant physical activity. We have demonstrated that IM is a feasible and helpful method for providing an evidence based and theoretical structure to a complex health behaviour change intervention. The next stage will be to assess the impact of the intervention on behaviour change and clinical factors associated with childhood obesity. The HAPPY programme is currently being tested as part of a randomised controlled feasibility trial.

  7. An economic evaluation of a self-management programme of activity, coping and education for patients with chronic obstructive pulmonary disease.

    Science.gov (United States)

    Dritsaki, Melina; Johnson-Warrington, Vicki; Mitchell, Katy; Singh, Sally; Rees, Karen

    2016-02-01

    The aim was to undertake a cost-utility analysis of a self-management programme of activity, coping and education (SPACE) for chronic obstructive pulmonary disease (COPD). The analysis was conducted alongside a six-month randomized controlled trial in 30 primary care settings. The economic analysis used data from 184 patients with confirmed diagnosis of COPD, forced expiratory volume in one second/forced vital capacity ratio management programme consisting of an education manual (SPACE for COPD) and consultation or usual care. Six-month costs were estimated from the National Health Service and Personal Social Services perspective and quality-adjusted life years (QALYs) were calculated based on patient responses at baseline and six months.The mean difference in costs between usual care and SPACE FOR COPD programme was -£27.18 (95% confidence interval (CI); -£122.59 to £68.25) while mean difference in QALYs was -0.10 (95% CI; -0.17 to -0.02). The results suggest that the intervention is more costly and more effective than usual care. The probability of the intervention being cost-effective was 97% at a threshold of £20,000/QALY gained. We conclude that the SPACE FOR COPD programme is cost-effective compared to usual care.

  8. Smoking cessation programmes using traditional medicine in Korea.

    Science.gov (United States)

    Jang, Soobin; Park, Yu Lee; Lee, Ju Ah; Kim, Kyeong Han; Lee, Eun-Kyoung; Sun, Seung-Ho; Shin, Yong-Cheol; Ko, Seong-Gyu; Park, Sunju

    2016-12-01

    There are growing interests in using various methods including traditional and complementary medicines (T&CM) for tobacco control. The study aimed to introduce how traditional Korean medicine (TKM) applied to smoking cessation programmes in Korea and to show the detail information of each programme for designing other smoke cessation programmes. Reports of the smoke cessation programmes in Korea were searched on March 10th, 2016, from the webpages of the related agencies and the databases: the Ministry of Health and Welfare, the Korea Health Foundation, the Association of Korean Medicine, PubMed, Google scholar, the RISS, the KISS, the NDSL, and the OASIS. Smoking cessation programmes, projects, or services using traditional Korean medicine (TKM) were included with no language, implementation site, and year restrictions. The three smoking cessation programmes using TKM in South Korea were the public health centre smoking cessation programme (PHC-SCP), the Ministry of Gender Equality & Family smoking cessation programme (MOGEF-SCP), and the National Health Insurance Service smoking cessation treatment project (NHIS-SCP). All programmes included ear acupuncture and counselling. Manual acupuncture was only used in the NHIS-SCP. The MOGEF-SCP and the NHIS-SCP used herbal medicines selectively. The PHC-SCP and MOGEF-SCP provided education programme and other tools such as non-smoking doll, self-writing handbook. They were run at no cost for participants. Treatment period were different for each programmes, 3 weeks, 4 weeks, 8 to 12 weeks, respectively. Treatment frequency was twice a week for PHC-SCP and MOGEF-SCP, and dependent on each clinic for NHIS-SCP. This study showed the summaries of the smoking cessation programme that used TKM. The three programmes and the detail information will be a reference for other countries that are going to apply T&CM to their smoking cessation programme. Though TKM integrated smoking cessation programmes had been contributed to

  9. Expression of Kiss-1 gene in breast tumor tissues and its clinical significance%Kiss-1基因在乳腺肿瘤组织中的表达及其临床意义

    Institute of Scientific and Technical Information of China (English)

    谢菲; 周波; 杨德启

    2012-01-01

    Objective To evaluate the expression of Kiss-1 mRNA in normal breast tissues and breast tumor tissues and its clinical significance. Methods The expression of Kiss-1 mRNA was detected by reverse transcription- polymerase chain reaction (RT-PCR) in normal breast tissues and breast tumor tissues of 70 patients with breast cancer and 40 patients with fibroadenoma. Results The expression of Kiss-1 mRNA in breast cancer tissues (0. 952 ± 0. 576) and fibroadenoma tissues (0. 587 ±0.496) were higher than that in adjacent normal tis8ues(t=7. 911 ,P=0.00;t = 2. 656,p=0. 011). The expression of Kiss-1 mRNA in breast cancer tissues was higher than that in fibroadenoma tissues (t = 3.365, P = 0. 001). The expression of Kiss-1 mRNA in breast cancer patients with lymph node metastasis (0. 662 ±0.530), was lower than that in patients without lymph node metastasis (1. 124±0.537) ((=3.495, P=0. 001). Conclusion With the relatively high expression in breast tumor tissues, Kiss-1 mRNA may serve as a new marker of breast tumor. The low expression of Kiss-1 mRNA may be related to lymph node metastasis of breast cancer.%目的 探讨Kiss-1 mRNA在乳腺肿瘤组织和正常腺体组织中的表达及其临床意义.方法 采用逆转录聚合酶链式反应(RT-PCR)法检测70例乳腺癌、40例乳腺纤维腺瘤及肿瘤旁正常腺体中Kiss-1 mRNA的表达,采用配对t检验、成组t检验及方差分析比较各组间Kiss-1 mRNA的相对表达水平.结果 Kiss-1 mRNA在乳腺癌组织及纤维腺瘤组织中的表达量分别为(0.952±0.576)、(0.587±0.496),均高于正常腺体组织(t=7.917,P=0.00;t=2.656,P=0.011),乳腺癌组织中的表达量高于纤维腺瘤组织(t=3.365,P=0.001).有淋巴结转移的乳腺癌组织中Kiss-1 mRNA表达量为(0.662±0.530),低于无淋巴结转移乳腺癌组织(1.124+0.537)(t=3.495,P=0.001).结论 Kiss-1 mRNA在乳腺肿瘤组织中相对高表达,可能成为潜在的乳腺肿瘤标志物;Kiss-1 mRNA低表达可能与乳腺癌淋巴结转移相关.

  10. Percutaneous reconstruction of the innominate bifurcation using the retrograde 'kissing stents' technique

    Energy Technology Data Exchange (ETDEWEB)

    Nagata, Shun-ichi; Kazekawa, Kiyoshi; Matsubara, Shuko [Fukuoka University Chikushi Hospital, Department of Neurosurgery, Chikushino, Fukuoka (Japan); Sugata, Sei [Bironoki Neurosurgical Hospital, Shibushi, Kagoshima (Japan)

    2006-08-15

    Obstructions of the supraaortic vessels are an important cause of morbidity associated with a variety of symptoms. Percutaneous transluminal angioplasty has evolved as an effective and safe treatment modality for occlusive lesions of the supraaortic vessels. However, the endovascular management of an innominate bifurcation has not previously been reported. A 53-year-old female with a history of systematic hypertension, diabetes mellitus and hypercholesterolemia presented with left hemiparesis and dysarthria. Angiography of the innominate artery showed a stenosis of the innominate bifurcation. The lesion was successfully treated using the retrograde kissing stent technique via a brachial approach and an exposed direct carotid approach. The retrograde kissing stent technique for the treatment of a stenosis of the innominate bifurcation was found to be a safe and effective alternative to conventional surgery. (orig.)

  11. Kissing-stents technique after living-donor lobar lung transplantation.

    Science.gov (United States)

    Sugimoto, Seiichiro; Oto, Takahiro; Toyooka, Shinichi; Miyoshi, Shinichiro

    2015-06-01

    Stent placement has become common practice for bronchial stenosis (BS) after lung transplantation (LT). Especially, segmental BS after lobar LT requires a complex stenting technique. We describe a case of multiple segmental bronchial stenoses treated by the kissing-stents technique using balloon-expandable metallic stents after living-donor lobar LT. Based on the vascular kissing-stents technique, we simultaneously placed two stents, side by side, in the superior segmental bronchus and the basal segmental bronchus of the right transplanted lobar lung. This technique may represent a valuable option for complex segmental BS after lobar LT. © The Author 2014. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.

  12. Effects of 6-month soccer and traditional physical activity programmes on body composition, cardiometabolic risk factors, inflammatory, oxidative stress markers and cardiorespiratory fitness in obese boys.

    Science.gov (United States)

    Seabra, André; Katzmarzyk, Peter; Carvalho, Maria José; Seabra, Ana; Coelho-E-Silva, Manuel; Abreu, Sandra; Vale, Susana; Póvoas, Susana; Nascimento, Henrique; Belo, Luís; Torres, Sandra; Oliveira, José; Mota, Jorge; Santos-Silva, Alice; Rêgo, Carla; Malina, Robert M

    2016-10-01

    Physical activity is important in obesity prevention, but the effectiveness of different physical activity modalities remains to be determined among children. The main purpose of this study was to compare the effects of a 6-month soccer programme and a traditional physical activity programme on changes in body composition, cardiometabolic risk factors, inflammatory and oxidative markers, cardiorespiratory fitness and perceived psychological status in obese boys. Eighty-eight boys (8-12 years; BMI > +2 standard deviations of WHO reference values) participated in one of three groups: soccer, traditional activity and control. Soccer and traditional activity programmes involved 3 sessions per week for 60-90 min at an average intensity of 70-80% of maximal heart rate. Control group participated in activities of normal daily living. All boys participated in school physical education, two sessions per week of 45-90-min. Measurements were taken at baseline and after 6 months, and included body size and composition, cardiometabolic risk factors, inflammatory and oxidative markers, cardiorespiratory fitness and perceived psychological status. Physical activity and dietary intake were assessed before and immediately following the intervention. The three groups had similar characteristics at baseline. After 6 months, both intervention groups had significantly lower relative fatness (% fat), waist circumference and total cholesterol, and higher cardiorespiratory fitness, self-esteem, perceived physical competence and attraction to physical activity compared with control group. In conclusion, physical activity interventions over 6 months positively influenced several indicators of health status among obese boys. The results also suggested that soccer has the potential as an effective tool for the prevention and reduction of childhood obesity and associated consequences.

  13. Feasibility and acceptability of a midwife-led intervention programme called 'Eat Well Keep Active' to encourage a healthy lifestyle in pregnancy

    Directory of Open Access Journals (Sweden)

    Warren Lucie

    2012-04-01

    Full Text Available Abstract Background Eating a diet that is high in fat and sugar and having a sedentary lifestyle during pregnancy is understood to increase the risk of excessive gestational weight gain and obesity following the birth of the baby. However, there are no clinical guidelines in the UK on what is considered to be appropriate gestational weight gain. Indeed, clinical recommendations discourage the routine re-weighing of pregnant women, stating instead that women should be advised regarding their diet and activity levels, in order to prevent excessive weight gain. Pregnancy is seen as a time when many women may have an increased motivation to improve their lifestyle behaviours for the benefit of the fetus. However, it is evident that many women have difficulty in both maintaining a healthy balanced diet and remaining active through pregnancy. It would seem that midwives may be ideally placed to assist women to make and maintain healthier lifestyle choices during pregnancy. Methods/design This study will look at the feasibility and acceptability of a newly devised intervention programme called 'Eat Well Keep Active'. Participants will complete a questionnaire prior to the programme to obtain baseline data on food frequency, physical activity and to gauge their perception of personal ability to improve/maintain healthy lifestyle. The programme comprises client centred techniques; motivational interviewing and goal setting delivered early in pregnancy (12-16 weeks with the aim of supporting a healthy well balanced diet and either continuing or commencing appropriate levels of physical activity. Participants will then be followed up six weeks following the intervention with a one-to-one interview, and a further brief questionnaire. The interview will provide preliminary data regarding perceived effectiveness and acceptability of the 'Eat Well Keep Active' programme whilst the questionnaire will provide data regarding changes in the confidence of

  14. Contralateral approach to iliac artery recanalization with kissing nitinol stents present in the aortic bifurcation.

    Science.gov (United States)

    Joseph, George; Hooda, Amit; Thomson, Viji Samuel

    2015-01-01

    A 69-year-old man, who had earlier undergone reconstruction of the aortic bifurcation with kissing nitinol stents, presented with occlusion of the left external iliac artery. The occlusion was successfully and safely recanalized using contralateral femoral approach with passage of interventional hardware through the struts of the stents in the aortic bifurcation. Presence of contemporary flexible nitinol stents with open-cell design in the aortic bifurcation is not a contraindication to the use of the contralateral femoral approach.

  15. Kissing loop interaction in adenine riboswitch: insights from umbrella sampling simulations.

    Science.gov (United States)

    Di Palma, Francesco; Bottaro, Sandro; Bussi, Giovanni

    2015-01-01

    Riboswitches are cis-acting regulatory RNA elements prevalently located in the leader sequences of bacterial mRNA. An adenine sensing riboswitch cis-regulates adeninosine deaminase gene (add) in Vibrio vulnificus. The structural mechanism regulating its conformational changes upon ligand binding mostly remains to be elucidated. In this open framework it has been suggested that the ligand stabilizes the interaction of the distal "kissing loop" complex. Using accurate full-atom molecular dynamics with explicit solvent in combination with enhanced sampling techniques and advanced analysis methods it could be possible to provide a more detailed perspective on the formation of these tertiary contacts. In this work, we used umbrella sampling simulations to study the thermodynamics of the kissing loop complex in the presence and in the absence of the cognate ligand. We enforced the breaking/formation of the loop-loop interaction restraining the distance between the two loops. We also assessed the convergence of the results by using two alternative initialization protocols. A structural analysis was performed using a novel approach to analyze base contacts. Contacts between the two loops were progressively lost when larger inter-loop distances were enforced. Inter-loop Watson-Crick contacts survived at larger separation when compared with non-canonical pairing and stacking interactions. Intra-loop stacking contacts remained formed upon loop undocking. Our simulations qualitatively indicated that the ligand could stabilize the kissing loop complex. We also compared with previously published simulation studies. Kissing complex stabilization given by the ligand was compatible with available experimental data. However, the dependence of its value on the initialization protocol of the umbrella sampling simulations posed some questions on the quantitative interpretation of the results and called for better converged enhanced sampling simulations.

  16. The Results of Self-Expandable Kissing Stents in Aortic Bifurcation.

    Science.gov (United States)

    Moon, Jae Young; Hwang, Hong Pil; Kwak, Hyo Sung; Han, Young Min; Yu, Hee Chul

    2015-03-01

    Kissing stent reconstruction is a widely used technique for the management of aortoiliac occlusive disease involving the aortic bifurcation or proximal common iliac arteries. The purpose of this study was to evaluate the results of self-expandable kissing stents in the aortic bifurcation. We reviewed medical records of the patients treated with a kissing stent retrospectively from January 2007 to December 2012. The primary and secondary patencies were determined with Kaplan-Meier analysis, and Cox regression was used to determine the factors associated with patency. A total of 21 patients were included, and all were male (median age 53±15 years, range 48-78 years). Major symptoms were claudication (n=16, 61.9%), rest pain (n=5, 23.8%) and gangrene (n=5, 23.8%). Tans-Atlantic Inter-Society Consensus for the Management of Peripheral Arterial Disease (TASC) II classification was A 2 (9.5%), B 5 (23.8%), C 7 (33.3%) and D 8 (38%). The mean follow-up was 40.7 months. Major complication occurred in only one case which consisted of distal limb ischemia by emboli. Six patients developed symptomatic restenosis or occlusion. There was no major amputation, but minor amputation occurred in 3 patients. There were 2 mortalities not associated with the procedure (lung cancer and intracranial hemorrhage). Primary patency was 89.6% at 1 year, 74.7% at 3 years and 64.0% at 5 years. Secondary patency was 94.1% at 1 year, 88.2% at 3 years and 68.6% at 5 years. No risk factors for restenosis or occlusion were identified. Self-expandable kissing stents can be used successfully with comparable patency for endovascular treatment of symptomatic atherosclerotic occlusive lesions in the aortic bifurcation area.

  17. Development and implementation of a lifestyle intervention to promote physical activity and healthy diet in the Dutch general practice setting: the BeweegKuur programme

    Directory of Open Access Journals (Sweden)

    de Vries Nanne K

    2010-05-01

    Full Text Available Abstract Background The number of patients with diabetes is increasing. BeweegKuur (Dutch for 'Exercise Therapy' is a Dutch lifestyle intervention which aims to effectively and feasibly promote physical activity and better dietary behaviour in primary health care to prevent diabetes. Methods The goal of this paper is to present the development process and the contents of the intervention, using a model of systematic health promotion planning. The intervention consists of a 1-year programme for diabetic and prediabetic patients. Patients are referred by their general practitioner (GP to a lifestyle advisor (LSA, usually the practice nurse or a physiotherapist. Based on specific inclusion criteria and in close collaboration with the patient, an individual exercise programme is designed and supervised by the LSA. This programme can be attended at existing local exercise facilities or (temporarily under the supervision of a specialized exercise coach or physiotherapist. All participants are also referred to a dietician and receive diet-related group education. In the first pilot year (2008, the BeweegKuur programme was implemented in 7 regions in the Netherlands (19 GP practices and health centres, while 14 regions (41 GP practices and health centres participated during the second year. The aim is to implement BeweegKuur in all regions of the Netherlands by 2012. Discussion The BeweegKuur programme was systematically developed in an evidence- and practice-based process. Formative monitoring studies and (controlled effectiveness studies are needed to examine the diffusion process and the effectiveness and cost-effectiveness of the intervention.

  18. IDIOPATHIC THORACO SCOLIOSIS TREATMENT USING A COMBINATION OF DOCUMENTATION BASED CARE (DBC BACK ACTIVE RECONDITIONING PROGRAMME AND SCHROTH METHODS: A PROSPECTIVE CASE STUDY

    Directory of Open Access Journals (Sweden)

    Ganesan Kathiresan

    2013-10-01

    Full Text Available reconditioning programme and Schroth methods to lessen the curvature allied with idiopathic Thoracicscoliosis.ResearchDesign: Sampling-An 18 years old female, single case study,Setting-DBC Kuching Active rehabilitationcentre, Malaysia.Duration of the study-20 weeks,Outcome measures-A base line and follow-up outcomemeasures includes Borg pain scale, Functional Rating Index, Balance test, and Radiographic analysis.Interventions-Document Based Care (DBC Back active reconditioning programme and Schroth methodsConclusion-The combination of the Document Based Care (DBC Back active reconditioning programme andSchroth methods seemed to be effective at reducing a Thoracic dextroscoliosis by about 14° (50% andLumbarLevoscoliosis by about 7° (25%, Short form (SF-36 showed improvement inQuality of life,balance time on theBalance board improved to 52 seconds and Borg pain scale rated 8/10 at the onset of care, dropped to a 1/10after 20 weeks, in this single case study. The positive outcomes of this practice validate a policyof offeringconservative treatment as an alternative to Thoracic scoliosis patients, including those for whom surgery isdiscussed

  19. Re-thinking global health sector efforts for HIV and tuberculosis epidemic control: promoting integration of programme activities within a strengthened health system

    Directory of Open Access Journals (Sweden)

    Maher Dermot

    2010-07-01

    Full Text Available Abstract Background The global financial crisis threatens global health, particularly exacerbating diseases of inequality, e.g. HIV/AIDS, and diseases of poverty, e.g. tuberculosis. The aim of this paper is to reconsider established practices and policies for HIV and tuberculosis epidemic control, aiming at delivering better results and value for money. This may be achieved by promoting greater integration of HIV and tuberculosis control programme activities within a strengthened health system. Discussion HIV and tuberculosis share many similarities in terms of their disease burden and the recommended stratagems for their control. HIV and tuberculosis programmes implement similar sorts of control activities, e.g. case finding and treatment, which depend for success on generic health system issues, including vital registration, drug procurement and supply, laboratory network, human resources, and financing. However, the current health system approach to HIV and tuberculosis control often involves separate specialised services. Despite some recent progress, collaboration between the programmes remains inadequate, progress in obtaining synergies has been slow, and results remain far below those needed to achieve universal access to key interventions. A fundamental re-think of the current strategic approach involves promoting integrated delivery of HIV and tuberculosis programme activities as part of strengthened general health services: epidemiological surveillance, programme monitoring and evaluation, community awareness of health-seeking behavior, risk behaviour modification, infection control, treatment scale-up (first-line treatment regimens, drug-resistance surveillance, containing and countering drug-resistance (second-line treatment regimens, research and development, global advocacy and global partnership. Health agencies should review policies and progress in HIV and tuberculosis epidemic control, learn mutual lessons for policy

  20. HIV-1 DIS stem loop forms an obligatory bent kissing intermediate in the dimerization pathway.

    Science.gov (United States)

    Mundigala, Hansini; Michaux, Jonathan B; Feig, Andrew L; Ennifar, Eric; Rueda, David

    2014-06-01

    The HIV-1 dimerization initiation sequence (DIS) is a conserved palindrome in the apical loop of a conserved hairpin motif in the 5'-untranslated region of its RNA genome. DIS hairpin plays an important role in genome dimerization by forming a 'kissing complex' between two complementary hairpins. Understanding the kinetics of this interaction is key to exploiting DIS as a possible human immunodeficiency virus (HIV) drug target. Here, we present a single-molecule Förster resonance energy transfer (smFRET) study of the dimerization reaction kinetics. Our data show the real-time formation and dissociation dynamics of individual kissing complexes, as well as the formation of the mature extended duplex complex that is ultimately required for virion packaging. Interestingly, the single-molecule trajectories reveal the presence of a previously unobserved bent intermediate required for extended duplex formation. The universally conserved A272 is essential for the formation of this intermediate, which is stabilized by Mg(2+), but not by K(+) cations. We propose a 3D model of a possible bent intermediate and a minimal dimerization pathway consisting of three steps with two obligatory intermediates (kissing complex and bent intermediate) and driven by Mg(2+) ions. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

  1. Prevalence and persistence of male DNA identified in mixed saliva samples after intense kissing.

    Science.gov (United States)

    Kamodyová, Natália; Durdiaková, Jaroslava; Celec, Peter; Sedláčková, Tatiana; Repiská, Gabriela; Sviežená, Barbara; Minárik, Gabriel

    2013-01-01

    Identification of foreign biological material by genetic profiling is widely used in forensic DNA testing in different cases of sexual violence, sexual abuse or sexual harassment. In all these kinds of sexual assaults, the perpetrator could constrain the victim to kissing. The value of the victim's saliva taken after such an assault has not been investigated in the past with currently widely used molecular methods of extremely high sensitivity (e.g. qPCR) and specificity (e.g. multiplex Y-STR PCR). In our study, 12 voluntary pairs were tested at various intervals after intense kissing and saliva samples were taken from the women to assess the presence of male DNA. Sensitivity-focused assays based on the SRY (single-copy gene) and DYS (multi-copy gene) sequence motifs confirmed the presence of male DNA in female saliva after 10 and even 60min after kissing, respectively. For specificity, standard multiplex Y-STR PCR profiling was performed and male DNA was found in female saliva samples, as the entire Y-STR profile, even after 30min in one sample. Our study confirms that foreign DNA tends to persist for a restricted period of time in the victim's mouth, can be isolated from saliva after prompt collection and can be used as a valuable source of evidence. Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

  2. ELAKCA: Enzyme-Linked Aptamer Kissing Complex Assay as a Small Molecule Sensing Platform.

    Science.gov (United States)

    Chovelon, Benoit; Durand, Guillaume; Dausse, Eric; Toulmé, Jean-Jacques; Faure, Patrice; Peyrin, Eric; Ravelet, Corinne

    2016-03-01

    We report herein a novel sandwich-type enzyme-linked assay for the "signal-on" colorimetric detection of small molecules. The approach (referred to as enzyme-linked aptamer kissing complex assay (ELAKCA)) relied on the kissing complex-based recognition of the target-bound hairpin aptamer conformational state by a specific RNA hairpin probe. The aptamer was covalently immobilized on a microplate well surface to act as target capture element. Upon small analyte addition, the folded aptamer was able to bind to the biotinylated RNA hairpin module through loop-loop interaction. The formed ternary complex was then revealed by the introduction of the streptavidin-horseradish peroxidase conjugate that catalytically converted the 3,3',5,5'-tetramethylbenzidine substrate into a colorimetric product. ELAKCA was successfully designed for two different systems allowing detecting the adenosine and theophylline molecules. The potential practical applicability in terms of biological sample analysis (human plasma), temporal stability, and reusability was also reported. Owing to the variety of both hairpin functional nucleic acids, kissing motifs, and enzyme-based signaling systems, ELAKCA opens up new prospects for developing small molecule sensing platforms of wide applications.

  3. Early and midterm results of kissing stent technique in the management of aortoiliac obstructive disease.

    Science.gov (United States)

    Pulli, Raffaele; Dorigo, Walter; Fargion, Aaron; Angiletta, Domenico; Azas, Leonidas; Pratesi, Giovanni; Alessi Innocenti, Alessandro; Pratesi, Carlo

    2015-04-01

    To retrospectively analyze the early and the midterm results of endovascular management of aortoiliac obstructive disease with the kissing stent technique. From January 2005 to September 2012, 229 consecutive endovascular interventions for aortoiliac obstructive disease were performed; data from all the interventions were prospectively collected in a dedicated database. In 41 patients, the kissing stent technique at the level of aortic bifurcation was performed (group 1), whereas in the remaining 188 it was not (group 2). Perioperative results were compared with chi-squared test. Follow-up results were analyzed with Kaplan-Meier curves and compared with log-rank test. Trans-Atlantic Inter-Society Consensus II C and D lesions were present in 66% of patients in group 1 and in 28.5% in group 2 (P kissing stent technique provided satisfactory results in patients with obstructive aortoiliac diseases, without an increase in immediate and midterm complications, representing an effective solution in complex anatomies. Copyright © 2015 Elsevier Inc. All rights reserved.

  4. [Congenital "kissing" lesions: Nevus or "café au lait" spot?

    Science.gov (United States)

    Durazzo, A; Boccara, O; Fraitag, S; Fusade, T; Picard, A; Kadlub, N

    2016-12-01

    "Café au lait" spots (CLS) are pigmented skin lesions principally located at the trunk and the limbs. Histologically, CLSs consist in an excessive pigmentation of the epidermis, with no risk of malignant transformation. The "kissing" nevus is a rare pigmented congenital nevus affecting both lower and upper eyelids in a mirror layout. As other nevi, it presents a theoretical risk of malignant transformation. These two pigmented lesions are responsible for aesthetic discomfort when affecting the face. Three patients presenting with a congenital pigmented lesion affecting the two eyelids in a mirror layout are presented. In two cases, the lesions, initially considered as "kissing" nevi, were classified as CLSs. The diagnosis of CLS was made on a biopsy in one patient and after surgery in the other one. Pigmented mirror layout lesions, called "kissing" lesions, are exclusively described for the nevi. We describe two cases of CLSs affecting the eyelids in a mirror layout. Difficulties in diagnostic are exposed and the possible treatments are discussed. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  5. Endovascular Treatment of Occlusive Lesions in the Aortic Bifurcation with Kissing Polytetrafluoroethylene-Covered Stents.

    Science.gov (United States)

    Grimme, Frederike A B; Spithoven, J Hans; Zeebregts, Clark J; Scharn, Dirk M; Reijnen, Michel M P J

    2015-09-01

    To determine the clinical outcomes of polytetrafluoroethylene covered balloon expandable stents (CBESs) in occlusive lesions of the aortic bifurcation in a kissing stent configuration. The study included 69 consecutive patients (29 men, 40 women) who underwent kissing stent procedures with CBESs between January 2003 and April 2009 in a single center. Patients who were previously treated with a CBES were excluded. Follow-up consisted of clinical investigation and duplex ultrasound examination. The primary patency was 88.1% at 1 year and 71.5% at 4 years, with secondary patency rates of 88.1% and 75.3%, respectively. For patients receiving a stent for the first time, primary patency was 91.3% at 1 year and 77.1% at 4 years. For patients who had received previous stents, patency was 83.6% at 1 year and 65.2% at 4 years (P = .83). There were no differences in secondary patency and freedom from target lesion reintervention (TLR). Loss of primary patency was mainly caused by stent occlusions (14 cases [78%]). The freedom from TLR at 4 years was 76.8%. Patency rates and freedom from TLR of CBESs in the kissing stent configuration with up to 4 years of follow-up were satisfying and mainly affected by stent occlusions. Studies focusing on optimizing stent configuration and medical care to reduce the incidence of thrombosis are indicated to improve results further. Copyright © 2015 SIR. Published by Elsevier Inc. All rights reserved.

  6. Outcomes of the single-stent versus kissing-stents technique in asymmetric complex aortoiliac bifurcation lesions.

    Science.gov (United States)

    Suh, Yongsung; Ko, Young-Guk; Shin, Dong-Ho; Kim, Jung-Sun; Kim, Byeong-Keuk; Choi, Donghoon; Hong, Myeong-Ki; Jang, Yangsoo

    2015-07-01

    This study investigated the outcomes of single-stent vs kissing-stents techniques in asymmetric complex aortoiliac bifurcation (ACAB) lesions. We retrospectively investigated 80 consecutive patients (69 males, 66.6 ± 8.7 years) treated with a single stent and 30 patients (26 males, 67.1 ± 7.7 years) treated with kissing stents for ACAB between January 2005 and December 2012 from a single-center cohort. A ACAB lesion was defined as a symptomatic unilateral common iliac artery stenosis (>50%) combined with intermediate stenosis (30%-50%) in the contralateral common iliac artery ostium. The primary end point was the primary patency of the ACAB. The baseline clinical characteristics did not differ significantly between the single-stent and the kissing-stents group. Technical success was achieved in all patients. The single-stent group required fewer stents (1.3 ± 0.5 vs 2.3 ± 0.8; P kissing stents because of plaque shift to the contralateral side. The major complication rates were 8% in single-stent vs 13% in the kissing-stent group, which was similar (P = .399). At 3 years, the single-stent and kissing-stents group had similar rates of primary patency (89% vs 87%; P = .916) and target lesion revascularization-free survival (93% vs 87%; P = .462). The single-stent technique in ACAB was safe and showed midterm outcomes comparable with those of kissing stents. Considering the benefits, such as fewer stents, less bilateral femoral access, and the availability of contralateral access for future intervention, the single-stent technique may be an advantageous treatment option in ACAB. Copyright © 2015 Society for Vascular Surgery. Published by Elsevier Inc. All rights reserved.

  7. The ProActive trial protocol – a randomised controlled trial of the efficacy of a family-based, domiciliary intervention programme to increase physical activity among individuals at high risk of diabetes [ISRCTN61323766

    Directory of Open Access Journals (Sweden)

    Ekelund Ulf

    2004-10-01

    Full Text Available Abstract Background Increasing prevalence of obesity and disorders associated with sedentary living constitute a major global public health problem. While previous evaluations of interventions to increase physical activity have involved communities or individuals with established disease, less attention has been given to interventions for individuals at risk of disease. Methods/design ProActive aims to evaluate the efficacy of a theoretical, evidence- and family-based intervention programme to increase physical activity in a sedentary population, defined as being at-risk through having a parental family history of diabetes. Primary care diabetes or family history registers were used to recruit 365 individuals aged 30–50 years, screened for activity level. Participants were assigned by central randomisation to three intervention programmes: brief written advice (comparison group, or a psychologically based behavioural change programme, delivered either by telephone (distance group or face-to-face in the family home over one year. The protocol-driven intervention programme is delivered by trained facilitators, and aims to support increases in physical activity through the introduction and facilitation of a range of self-regulatory skills (e.g. goal setting. The primary outcome is daytime energy expenditure and its ratio to resting energy expenditure, measured at baseline and one year using individually calibrated heart rate monitoring. Secondary measures include self-report of individual and family activity, psychological mediators of behaviour change, physiological and biochemical correlates, acceptability, and costs, measured at baseline, six months and one year. The primary intention to treat analysis will compare groups at one-year post randomisation. Estimation of the impact on diabetes incidence will be modelled using data from a parallel ten-year cohort study using similar measures. Discussion ProActive is the first efficacy trial of an

  8. Data for action: the use of formative research to design a school-based intervention programme to increase physical activity in adolescents.

    Science.gov (United States)

    Murillo Pardo, Berta; Camacho-Miñano, Maria José; Generelo Lanaspa, Eduardo; Julián Clemente, José Antonio; Novais, Carina; Maia Santos, Maria Paula

    2015-09-01

    Formative research is a critical step for the development of interventions aimed at changing behaviours, as is the case of physical activity. This process permits obtaining detailed information about the programme application context. 'Follow the Footstep' is a quasi-experimental and longitudinal study in secondary schools, the aim of which is to increase levels of physical activity among adolescents. To inform the design of the intervention programme, formative research has been carried out to analyse the perceptions of parents, teachers and students, through six focus groups.The social-ecological model was used both to carry out the formative research and then to design the programme, including five levels of influence on behaviour (individual, interpersonal, organizational, community and political), which are important when it comes to adopting a comprehensive intervention approach. The authors describe how the results of formative research were transferred to guide the design and development of the intervention. As results indicate, parents, teachers and students agreed on a need to intervene by engaging adolescents and their close social environment. The school centre is the key organizational structure to implement this intervention, supported by professionals and the community.

  9. Personal goals, group performance and ‘social’ networks: participants’ negotiation of virtual and embodied relationships in the ‘Workplace Challenge’ physical activity programme

    DEFF Research Database (Denmark)

    Evans, Adam Brian; Carter, Alice; Middleton, Geoff

    2016-01-01

    County Sports Partnerships (CSPs) epitomise the growing reliance upon building networks and partnerships in sports delivery. This study investigated how social networks were created and contested in a CSP-led programme entitled the ‘Workplace Challenge’ (WPC). The WPC used a web-platform to encou......County Sports Partnerships (CSPs) epitomise the growing reliance upon building networks and partnerships in sports delivery. This study investigated how social networks were created and contested in a CSP-led programme entitled the ‘Workplace Challenge’ (WPC). The WPC used a web...... in professional I–We identities, whereas virtual networks sometimes highlighted participants’ isolation. Moreover, emphasis upon competition within and between teams caused some participants to question their performance. Often, competition motivated engagement. For less active participants, constant comparison...

  10. A theory evaluation of an induction programme

    Directory of Open Access Journals (Sweden)

    Kenrick Hendricks

    2012-07-01

    Full Text Available Orientation: An induction programme is commonly used to help new employees understand their job within the organisation. Research purpose: The main aim of this study was to examine whether or not the programme theory of an induction programme was plausible and would lead to the intended outcomes as described by the programme manager.Motivation for the study: Induction training is one of the most common training programmes in an organisation. However, there is little research to evaluate whether or not the activities of an induction programme will lead to the intended outcomes of such a programme.Research design, approach and method: This theory evaluation used a descriptive design. One hundred and thirteen employees of a media company completed a ten-item, five-point Likert scale which measured their perceptions of the programme’s outcome, identification with the organisation and intentions to stay with the organisation.Main findings: From this theory evaluation it was apparent that an induction programme based on an implausible programme theory could be problematic. An implausible programme theory affects the design of the programme activities and unsuitable activities may not deliver the desired outcomes.Practical/managerial implications: The intention of the evaluation is to guide human resource managers through a process of replacing an implausible programme theory with one that is plausible, and which ensures better alignment of programme activities and outcomes.Contribution/value-add: The evaluators showed how a plausible programme theory could improve programme design. This redesigned induction programme may lead to benefits, such as staff retention and company identification, rather than the vague assumption that it has been conforming to a legal obligation.

  11. The effects of a home-based arm ergometry exercise programme on physical fitness, fatigue and activity in polio survivors: protocol for a randomised controlled trial

    Directory of Open Access Journals (Sweden)

    Murray Deirdre

    2012-12-01

    Full Text Available Abstract Background Many Polio survivors have reduced mobility, pain and fatigue, which make access to conventional forms of aerobic exercise difficult. Inactivity leads to increased risk of health problems, many of which are prevalent among Polio survivors. Aerobic exercise programmes in Polio survivors should utilise stable muscle groups and should be designed to minimise exacerbation of pain and fatigue. A home-based arm ergometry aerobic exercise programme may represent an affordable and accessible exercise modality, incorporating exercise prescription principles in this group. Methods/design This is a prospective, single blinded, randomised controlled trial. There are two arms; exercise intervention using arm ergometers and control. Polio survivors meeting eligibility criteria will be recruited and randomly allocated to intervention or control groups. Participants allocated to the intervention group will receive a small arm ergometer and a polar heart rate monitor. They will carry out a home-based moderate intensity (50-70% HRMax aerobic exercise programme for eight weeks, following instruction by the treating physiotherapist. Assessments will occur at baseline and after eight weeks and will include tests of physical fitness, activity, energy cost of walking, fatigue and quality of life. Clinically feasible assessment tools including the Six Minute Arm Test, the Physical Activity Scale for People with Physical Disabilities questionnaire, the Physiological Cost Index, Fatigue Severity Scale and the SF-36v2 will be utilised. Discussion The efficacy of a home-based arm ergometry programme in Polio survivors will be examined. No previous trial has examined such a programme using a wide range of outcome measures pertinent to Polio survivors. This study will provide new information on the impact of arm ergometry on physical fitness, activity, body composition, fatigue, pain, muscle strength, and health related quality of life. Also, the study

  12. Angiographic and clinical outcomes after implantation of drug eluting stents in bifurcation lesions with crush or kissing stent technique.

    Science.gov (United States)

    Cheema, Asim N; Jolly, Sanjit S; Burstein, Jason M; Sharieff, Waseem; Mohammad, Atif; Yeoh, Eunice; Mancini, G B John; Cantor, Warren J; Kutryk, Michael J B; Strauss, Bradley H; Chisholm, Robert J

    2013-04-01

    Long-term outcome after bifurcation stenting with drug-eluting stents (DES) for obstructive coronary artery disease is poorly understood. In this study, we report 6-9-month angiographic follow-up and long-term clinical outcomes after implantation of drug-eluting stents by crush and kissing stent technique for coronary bifurcation lesions. Consecutive patients undergoing bifurcation stenting with DES by crush or kissing stent technique were enrolled in a prospective registry. Angiographic follow-up was obtained at 6-9 months and clinical follow-up completed for a median of 38 months. A total of 86 patients participated in the study. Bifurcation stenting by crush technique was performed in 73 (85%) and by kissing stent in 13 (15%) patients. Stenting of left main bifurcation was applied in 24 (28%) patients. Angiographic follow-up was completed in 75 (87%) patients and showed restenosis in the main for 8 (11%) and side branch for 20 (27%) patients. Clinical follow-up was available for a median duration of 38 months. During follow-up, 2 (2%) patients died, 4 (5%) experienced myocardial infarction (MI), and 11 (13%) underwent target vessel revascularization (TVR) with an overall major adverse cardiac event (MACE) rate of 16%. In left main cohort, angiographic restenosis occurred in 9 (37%) patients, and 3 (12%) patients required TVR. There were no deaths or stent thrombosis. A comparison of crush and kissing stent technique showed significantly higher angiographic restenosis with crush (26% vs 13% in kissing stent patients, P = 0.046) and 95% of restenosis in crush group involved ostium of the side branch. There was no difference in clinical outcomes between the crush and kissing stent groups. Final kissing balloon dilatation (FKB) was successful in 65 (89%) patients in the crush group and associated with a significant reduction in MACE (8% in FKB successful vs 37% in FKB unsuccessful, P = 0.04) during follow-up. Bifurcation stenting with crush or kissing stent

  13. ISOLDE PROGRAMME

    CERN Multimedia

    Fedosseev, V; Lynch, K M; Grob, L K; Herfurth, F; Scheidenberger, C; Geppert, C; Gorges, C; Ratajczyk, T; Vogel, S; Munch, M K; Nieminen, P; Pakarinen, J J A; Lecesne, N; Bouzomita, H; Grinyer, J; Marques moreno, F M; Parlog, M; Pedroza, J; Ghetta, V; Lozeva, R; Guillemaud mueller, D S; Cottereau, E; Cheikh mhamed, M; Tusseau nenez, S; Tungate, G; Walker, P M; Smith, A G; Fitzpatrick, C; Dominik, W M; Karny, M; Ciemny, A A; Nyman, G H; Thies, R M A; Lindberg, S K G; Langouche, G F; Delaure, B J P; Mayet, P; Ory, G T; Kesteloot, N J K; Papuga, J; Dehairs, M H R; Callens, M; Boudreau, M; Domnanich, K A; Richter, D; Lutter, R J; Wiehr, S; Nacher gonzalez, E; Jungclaus, A; Ribeiro jimenez, G; Marroquin alonso, I; Cal gonzalez, J; Paziy, V; Salsac, M; Murphy, C; Podolyak, Z F; Bajoga, A D; Butler, P; Pritchard, A; Steer, A N; Fox, S P; Wadsworth, B A; Truesdale, V L; Al monthery, M; Guttormsen, M S; Ingeberg, V W; Badea, M N; Calinescu, S; Cederkall, J A; Zemlyanoy, S; Donets, E D; Golovkov, M; 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Wadsworth, R; Cubiss, J G; Doherty, D T; Harding, R D; Vaintraub, S; Mandal, S K; Hadynska-klek, K; Scarpa, D; Hoff, P; Syed naeemul, H; Borcea, R; Balabanski, D L; Marginean, R; Rotaru, F; Rudolph, D; Fahlander, C H; Chudoba, V; Naidoo, D; Veselsky, M; Kliman, J; Raisanen, J A; Dietrich, M; Maung maung than, M M T; Reed, M W; Danchev, M T; Ray, J; Roy, M; Hammen, M; Capponi, L; Veghne csatlos, M M; Fryar, J; Mirzadeh vaghefi, S P; Trindade pereira, A M; Bakenecker, A; Tramm, C; Germic, V; Morel, P A; Kowalczyk, M; Matejska-minda, M; Ringvall moberg, A; Kana, T; Vermeeren, B R M; Dockx, K; Mantovan, R; Fransen, C H; Radeck, F; Schneiders, D W; Steinbach, T; Vibenholt, J E; Magnussen, M J; Stevnhoved, H M; Comas lijachev, V; Dasenbrock-gammon, N M; Perkowski, J; Matveev, Y; Wegner, M; Garcia borge, M J; Molholt, T E; Srnka, D; Dlouhy, Z; Beck, D; Homm, I; Eliseev, S; Blaum, K; Probst, M B; Kaiser, C J; Martin, J A; Refsgaard, J; Papadakis, F; Peura, P J; Greenlees, P T; Auranen, K; Delahaye, P; Traykov, E K; Perez loureiro, D; Mery, A A; Couratin, C; Tsekhanovich, I; Lunney, D; Gaulard, C V; Althubiti, N A S; Mottram, A D; Das, S K; Van de walle, J; Mazzocchi, C; Jonson, B N G; Woehr, A; Lesher, S R; Zuber, K T; Koudriavtsev, I; De witte, H J; Van den bergh, P A M; Raabe, R; Depuydt, M J F; Radulov, D P; Elseviers, J; Reynders, K L T; Sels, S M C; Verlinde, M; Delombaerde, L; De maesschalck, D; Dunlop, R A; Tarasava, K; Gernhaeuser, R A; Weinzierl, W; Berger, C; Wendt, K; Achtzehn, T; Gottwald, T; Schug, M; Rossel, R E; Dominguez reyes, R R; Briz monago, J A; Koester, U H; Bunce, M R; Bowry, M D; Nakhostin, M; Shearman, R; Cresswell, J R; Joss, D T; Gredley, A; Groombridge, D; Laird, A M; Aslanoglou, X; Siem, S; Weterings, J A; Renstrom, T; Szpak, B T; Luczkowski, M J; Ghita, D; Bezbakh, A; Bollmann, J; Bhattacharya, P; Roy, S; Rahaman, M A; Wlodarski, T; Carvalho soares, J; Barzakh, A; Werner, V R; Schertz, F; Froemmgen, N E; Liberati, V; Foy, B E; De pinho oliveira, G N; Weinheimer, C P; Zboril, M; Figuera, P; Simon, R E; Popescu, L A; Czosnyka, T; Miranda jana, P A; Buescher, J S L; Plociennik, W A; Ruchowska, E E; Chiara, C J; Eberth, J H; Thomas, T; Thole, P; Queiser, M T; Lo bianco, G; D'amico, F; Muller, S; Sanchez alarcon, R M; Tain enriquez, J L; Orrigo, S E A; Orlandi, R; Plazaola muguruza, F C; Pallada, S; Lepareur, N G; Wildner, E; Kowalska, M; Malbrunot, S; Slezak, M; Roeckl, E; Schrieder, G H; Ilieva, S K; Koenig, K L; Amoretti, M A; Lommen, J M; Fynbo, H O U; Weyer, G O P; Koldste, G T; Madsboll, K; Jensen, J H; Nieminen, A M; Reponen, M; Villari, A; Thomas, J; Saint-laurent, M; Sorlin, O H; Carniol, B; Pereira lopez, J; Grevy, S; Plaisir, C; Marie-jeanne, M J; Georgiev, G P; Etile, A M; Le blanc, F M; Verney, D; Stefan, G I; Assie, M; Suzuki, D; Guillot, J; Vazquez rodriguez, L; Campbell, P; Deacon, A N; Ware, T; Flueras, A; Xie, L; Banerjee, K; Piersa, M; Johansson, H T; Schwarz, S; Welker, A; Krauth, M R; Perrot, F; Aumont, J; Sferrazza, M; Van duppen, P L E; Versyck, S; Dehaes, J; Bree, N C F; Neyskens, P; Martinez palenzuela, Y; De groote, R P; Carlier, L M F; De schepper, S; Dewolf, K W A; Kabir, L R; Garcia ruiz, R F; Khodery ahmad, M A; Zadvornaya, A; Xu, Z; Smolders, P; Krastev, P; Rapisarda, E; Reber, J A; Mattolat, C F; Raeder, S; Habs, D; Martinez perez, T; Fraile prieto, L M; Vidal, M; Perez liva, M; Calvo portela, P; Ulla pedrera, F J; Wood, R T; Lalkovski, S; Page, R; Petri, M; Barton, C J; Nichols, A J; Vermeulen, M J; Bloor, D M; Henderson, J; Wilson, G L; De angelis, G; Buerger, A; Klintefjord, M L; Fornal, B A; Marginean, R; Sava, T; Suvaila, R; Lica, R; Costache, C; Mihai, R; Ionescu, A; Baeck, T M; Masenda, H; Sedlak, M; Koskelo, O K; Kyaw myat, K M; Ganguly, B; Goncalves marques, J; Cardoso, S; Seliverstov, M; Niessen, B D; Gutt, L E; Chapman, R; Spagnoletti, P N; Lopes, C; De oliveira amorim, C; Batista lopes, C M; Araujo, J; Schielke, S J; Daugas, J R; Gaudefroy, L; Chevrier, R; Szunyogh, D M; Napiorkowski, P J; Wrzosek-lipska, K; Wahl, U; Catarino, N; Pereira carvalho alves de sequeira, M; Decoster, S J; Porobic, T; Babo, M; Walters, W; Hess, H E; Holler, A; Bettermann, L; Geibel, K; Taprogge, J; Lewandowski, L T N; Manchado de sola, F; Das gupta, S; Thulstrup, P W; Heinz, U; Nogwanya, T; Neidherr, D M; Domingo pardo, C; Morales lopez, A I; Gumenyuk, O; Peaker, A R; Wakabayashi, Y; Abrahams, K J; Mach, H A; Souza ribeiro junior, I; He, J; Giles, T J; Dorsival, A; Kalaninova, Z; Venos, D; Kraemer, J; Saha, S; Neugart, R; Eronen, T O; Kreim, K D; Heck, M K; Goncharov, M; Julin, R J; Jakobsson, E H U; Eleon, C; Achouri, N L; Grinyer, G F; Fontbonne, C M; Alfaurt, P; Kusoglu, A; Wilkins, S G; Brown, A R; Imai, N; Pomorski, M J; Janiak, L; Nilsson, T; Stroke, H H; Stanja, J; Dangelser, E; Heenen, P; Mallion, S N; Diriken, J V J; Ghys, L H L; Khamehchi, M A; Van beveren, C; Gins, W A M; Bouma, J T; Koszorus, A; Mcnulty, J F; Ohlert, C M; Schwerdtfeger, W; Tengblad, O; Becerril reyes, A D; Perea martinez, A; Martinez perez, M C; Margerin, V; Rudigier, M; Alexander, T D; Patel, Z V; Hammond, N; Wearing, F; Patel, A; Jenkins, D G; Debernardi, A; Giacoppo, F; Tveten, G M; Malatji, K L; Krolas, W A; Stanoiu, M A; Rickert, E U; Ter-akopian, G; Cline, D; Riihimaeki, I A; Simon, K D; Wagner, F E; Turker, M; Neef, M H; Jakubek, J; Vagena, E; Bottoni, S; Nishimura, K; Correia, J; Rodrigues valdrez, C J; Ostrowski, A N; Hallmann, O; Scheck, M; Wady, P T; Lane, J; Krasznahorkay, A J; Kunne sohler, D; Meaney, A J; Baptista barbosa, M; Hochschulz, F; Roig, O; Houngbo, D; Behan, C C; Kargoll, S; Kemnitz, S; Redondo cubero, A; Dirkx, D; Stegemann, S T; Tallarida, G; Kaczarowski, R; Finke, F; Linnemann, A; Altenkirch, R; Saed-samii, N; Ansari, S H; Dlamini, W B; Adoons, V N; Ronning, C R; Wiedeking, M; Guadilla gomez, V; Herlert, A J; Mehl, C V; Judge, S M; Catherall, R; Lettry, J; Wenander, F J C; Zakoucky, D; Catchen, G L; Noertershaeuser, W; Kroell, T; Leske, J; Shubina, D; Murray, I M; Pancin, J; Delaunay, F; Poincheval, J J L; Audirac, L L; Gerbaux, M T; Aouadi, M; Sole, P G P; Fallot, M P; Onillon, A; Duchemin, C; Formento cavaier, R; Audi, G; Boukhari, A; Lau, C; Martin, J A; Barre, N H; Berry, T A; Procter, T J; Farooq-smith, G J; Bladen, L K; Axiotis, M; Muto, S; Jeong, S C; Hirayama, Y; Korgul, A B; Minamisono, K; Bingham, C R; Aprahamian, A; Bucher, B M; Huyse, M L; Himpe, P; Ferrer garcia, R; Sambi, S; Budincevic, I; Neven, M; Bomans, P; Romano, N; Maugeri, E A; Klupp, S C; Dehn, M H; Heinke, R M; Naubereit, P; Maira vidal, A; Vedia fernandez, M V; Ibanez garcia, P B; Bruyneel, B J E; Materna, T; Al-dahan, N; Alazemi, N; Carroll, R J; Babcock, C; Eleme, Z; Dhal, A; Valiente dobon, J J; Sahin, E; Goergen, A; Maj, A; Bednarczyk, P A; Borcea, C; Negoita, F; Suliman, G; Marginean, N M; Sotty, C O; Negret, A L; Nae, S A; Nita, C; Golubev, P I; Knyazev, A; Jost, C U; Petrik, K; Strisovska, J; Vaeyrynen, S A; Dracoulis, G D; Uher, J; Fernandez dominguez, B; Chakraborty, P; Avigo, R; Galaviz redondo, D; Castro ribeiro da silva, M; Bernards, C W; Falahat, S; Lekovic, F; Dorrer, H J; Derkx, X; Angus, L J; Sandhu, K S; Gregor, E; Byrne, D J; Haas, H; Lourenco, A A; Sousa pereira, S M; Sousa, J B; De melo mendonca, T M; Tavares de sousa, C; Guerreiro dos santos oliveira custodio, L M; Da rocha rodrigues, P M; Yamaguchi, T; Thompson, P C; Rosenbusch, M; Wienholtz, F; Fischer, P; Iwanicki, J S; Rusek, K M; Hanstorp, D; Severijns, N; Vanpoucke, B R S; Finlay, P E J; Park, S H; Warr, N V; Doornenbal, P C; Imig, A; Seidlitz, M; Moschner, K; Vogt, A; Kaya, L; Martel bravo, I; Orduz, A K; Serot, O; Majola, S N; Litvinov, Y; Bommert, M; Hensel, S; Markevich, V; Nishio, K; Ota, S; Matos, I; Zenkevich, A; Picado sandi, E; Forstner, O

    2002-01-01

    The experiments aim at a broad exploration of the properties of atomic nuclei far away from the region of beta stability. Furthermore, the unique radioactive beams of over 60~elements produced at the on-line isotope separators ISOLDE-2 and ISOLDE-3 are used in a wide programme of atomic, solid state and surface physics. Around 300 scientists are involved in the project, coming from about 70 laboratories. \\\\ \\\\ The electromagnetic isotope separators are connected on-line with their production targets in the extracted 600 MeV proton or 910~MeV Helium-3 beam of the Synchro-Cyclotron. Secondary beams of radioactive isotopes are available at the facility in intensities of 10$^1

  14. In-stent Anchoring Facilitating Side-branch Balloon Delivery for Final Kissing: A Prospective, Single-center Registry Study.

    Science.gov (United States)

    Zhou, Yu; Xiao, Han; Wang, Yu-Qing; Liu, Huan-Yun; Bao, Pang; Song, Yao-Ming; Azzalini, Lorenzo; Huang, Lan; Zhao, Xiao-Hui

    2016-11-20

    Recrossing the compromised side branch (SB) with a balloon is sometimes technically challenging. The aim of this study was to evaluate whether in-stent anchoring (ISA) is safe and effective to facilitate SB balloon delivery for final kissing. One hundred and fifty-nine consecutive patients were included (166 bifurcation lesions) in this prospective, single-center registry. ISA was used as a bailout method after unsuccessful SB crossing using conventional techniques, including low-profile balloons. Technique success was defined as SB balloon delivery and final kissing. Kissing-balloon delivery was successfully performed with conventional strategies in 149 of 166 lesions (89.8%). In the remaining 17 lesions (10.2%), recrossing of the main vessel stent strut was not successful; therefore, ISA was attempted. The balloon successfully crossed the stent struts, and final kissing was achieved in 15 of 17 lesions (88.2%). Total final kissing was achieved in 164 of 166 lesions (98.8%), with success rates of 100% in the single-stent group and 97.6% in the two-stent group. Two cases without balloon delivery had complex bifurcation lesions with severe calcification. There was no vessel dissection in the anchoring zone. ISA is safe and effective for recrossing stent struts when conventional low-profile balloons have failed. However, large-scale trials are warranted for further evaluation.

  15. Extremely Rare Form of Impaction Bilateral Kissing Molars: Report of a Case and Review of the Literature.

    Science.gov (United States)

    Zerener, Tamer; Bayar, Gurkan Rasit; Altug, Hasan Ayberk; Kiran, Serkan

    2016-01-01

    Kissing molars (KM) or rosette formation is a term that is used to describe impacted teeth contacting occlusal surfaces in a single follicular space and their roots pointing in opposite directions. In some cases kissing molars can be seen but occurrence of bilateral kissing molars is extremely rare phenomenon in the dental literature and the aetiology of this phenomenon is still unknown. In this paper we describe a case and review of the literature and discuss the management of this pathology. In our case, extremely rare form of impacted bilateral kissing molars was extracted surgically. The decision of extraction of asymptomatic kissing molars represents surgical dilemma. There may be many surgical complications; on the other hand in some cases surgical intervention is unavoidable. Few treatment options were described in the literature. This phenomenon can be sign of various medical conditions that may require further investigation. In this paper, our treatment option is in agreement with the literature suggesting the surgical removal of both teeth at either side of the mandible.

  16. Effectiveness of a Universal Parental Support Programme to Promote Healthy Dietary Habits and Physical Activity and to Prevent Overweight and Obesity in 6-Year-Old Children: The Healthy School Start Study, a Cluster-Randomised Controlled Trial: e0116876

    National Research Council Canada - National Science Library

    Gisela Nyberg; Elinor Sundblom; Åsa Norman; Benjamin Bohman; Jan Hagberg; Liselotte Schäfer Elinder

    2015-01-01

      Objective To develop and evaluate the effectiveness of a parental support programme to promote healthy dietary and physical activity habits and to prevent overweight and obesity in Swedish children...

  17. Unravelling the Evolution of the Allatostatin-Type A, KISS and Galanin Peptide-Receptor Gene Families in Bilaterians: Insights from Anopheles Mosquitoes.

    Directory of Open Access Journals (Sweden)

    Rute C Felix

    Full Text Available Allatostatin type A receptors (AST-ARs are a group of G-protein coupled receptors activated by members of the FGL-amide (AST-A peptide family that inhibit food intake and development in arthropods. Despite their physiological importance the evolution of the AST-A system is poorly described and relatively few receptors have been isolated and functionally characterised in insects. The present study provides a comprehensive analysis of the origin and comparative evolution of the AST-A system. To determine how evolution and feeding modified the function of AST-AR the duplicate receptors in Anopheles mosquitoes, were characterised. Phylogeny and gene synteny suggested that invertebrate AST-A receptors and peptide genes shared a common evolutionary origin with KISS/GAL receptors and ligands. AST-ARs and KISSR emerged from a common gene ancestor after the divergence of GALRs in the bilaterian genome. In arthropods, the AST-A system evolved through lineage-specific events and the maintenance of two receptors in the flies and mosquitoes (Diptera was the result of a gene duplication event. Speciation of Anopheles mosquitoes affected receptor gene organisation and characterisation of AST-AR duplicates (GPRALS1 and 2 revealed that in common with other insects, the mosquito receptors were activated by insect AST-A peptides and the iCa2+-signalling pathway was stimulated. GPRALS1 and 2 were expressed mainly in mosquito midgut and ovaries and transcript abundance of both receptors was modified by feeding. A blood meal strongly up-regulated expression of both GPRALS in the midgut (p < 0.05 compared to glucose fed females. Based on the results we hypothesise that the AST-A system in insects shared a common origin with the vertebrate KISS system and may also share a common function as an integrator of metabolism and reproduction.AST-A and KISS/GAL receptors and ligands shared common ancestry prior to the protostome-deuterostome divergence. Phylogeny and gene

  18. Unravelling the Evolution of the Allatostatin-Type A, KISS and Galanin Peptide-Receptor Gene Families in Bilaterians: Insights from Anopheles Mosquitoes.

    Science.gov (United States)

    Felix, Rute C; Trindade, Marlene; Pires, Isa R P; Fonseca, Vera G; Martins, Rute S; Silveira, Henrique; Power, Deborah M; Cardoso, João C R

    2015-01-01

    Allatostatin type A receptors (AST-ARs) are a group of G-protein coupled receptors activated by members of the FGL-amide (AST-A) peptide family that inhibit food intake and development in arthropods. Despite their physiological importance the evolution of the AST-A system is poorly described and relatively few receptors have been isolated and functionally characterised in insects. The present study provides a comprehensive analysis of the origin and comparative evolution of the AST-A system. To determine how evolution and feeding modified the function of AST-AR the duplicate receptors in Anopheles mosquitoes, were characterised. Phylogeny and gene synteny suggested that invertebrate AST-A receptors and peptide genes shared a common evolutionary origin with KISS/GAL receptors and ligands. AST-ARs and KISSR emerged from a common gene ancestor after the divergence of GALRs in the bilaterian genome. In arthropods, the AST-A system evolved through lineage-specific events and the maintenance of two receptors in the flies and mosquitoes (Diptera) was the result of a gene duplication event. Speciation of Anopheles mosquitoes affected receptor gene organisation and characterisation of AST-AR duplicates (GPRALS1 and 2) revealed that in common with other insects, the mosquito receptors were activated by insect AST-A peptides and the iCa2+-signalling pathway was stimulated. GPRALS1 and 2 were expressed mainly in mosquito midgut and ovaries and transcript abundance of both receptors was modified by feeding. A blood meal strongly up-regulated expression of both GPRALS in the midgut (p < 0.05) compared to glucose fed females. Based on the results we hypothesise that the AST-A system in insects shared a common origin with the vertebrate KISS system and may also share a common function as an integrator of metabolism and reproduction. AST-A and KISS/GAL receptors and ligands shared common ancestry prior to the protostome-deuterostome divergence. Phylogeny and gene synteny revealed

  19. Does a physiotherapy programme of gross motor training influence motor function and activities of daily living in children presenting with developmental coordination disorder?

    Directory of Open Access Journals (Sweden)

    Sonill S. Maharaj

    2016-02-01

    Full Text Available Background: Children with developmental coordination disorder (DCD lack motor coordination and have difficulty performing motor skills and activities of daily living. Research shows these children do not outgrow their motor difficulties and without intervention do not improve. Physiotherapy is relevant for these children, but due to limited clinical protocols for DCD the aim of this study was to determine the effect of a gross motor training programme for 6–12-year-old children with DCD.Methods: This randomised pre-test, post-test study recruited 64 children with scores of 15th percentile or below using the Movement Assessment Battery for Children (M-ABC. The children were divided equally into an intervention group receiving 8 weeks of gross motor training for core stability, strengthening exercises, balance and coordination with task-specific activities for 30 min per week, while the control group continued with general therapy and activities of daily living. The M-ABC and Developmental Coordination Disorder Questionnaire (DCDQ were used to assess each child before and after 8 weeks.Results: Sixty children completed the study, with 43 males and 17 females (mean age 10.02 years, SD = 2.10. There were no adverse reactions to the programme and M-ABC scores for the intervention programme improved by 6.46%, ball skills (3.54% and balance (4.80% compared with the control (0.17% and (0.15%, respectively. There were significant (p < 0.05 improvements in DCDQ scores, but teachers allocated lower scores than parents.Conclusion: This study supports 8 weeks of gross motor training which can be a beneficial intervention for physiotherapists to improve gross motor function for DCD.Keywords: Developmental, co-ordination, skills, motor

  20. On the conformational stability of the smallest RNA kissing complexes maintained through two G·C base pairs.

    Science.gov (United States)

    Chu, Wally; Weerasekera, Akila; Kim, Chul-Hyun

    2017-01-29

    Two identical 5'GACG3' tetra-loop motifs with different stem sequences (called H2 and H3) are found in the 5' end region of Moloney Murine Leukemia Virus (MMLV) genomic RNA. They play important roles in RNA dimerization and encapsidation through two identical tetra-loops (5'GACG3') forming a loop-to-loop kissing complex, the smallest RNA kissing complex ever found in nature. We examined the effects of a loop-closing base pair as well as a stem sequence on the conformational stability of the kissing complex. UV melting analysis and gel electrophoresis were performed on eight RNA sequences mimicking the H2 and H3 hairpin tetra-loops with variation in loop-closing base pairs. Our results show that changing the loop-closing base pair from the wildtype (5'A·U3' for H3, 5'U·A3' for H2) to 5'G·C3'/5'C·G3' has significant effect on the stability of the kissing complexes: the substitution to 5'C·G3' significantly decreases both thermal and mechanical stability, while switching to the 5'G·C3' significantly increases the mechanical stability only. The kissing complexes with the wildtype loop-closing base pairs (5'A·U3' for H3 and 5'U·A3' for H2) show different stability when attached to a different stem sequence (H2 stem vs. H3 stem). This suggests that not only the loop-closing base pair itself, but also the stem sequence, affects the conformational stability of the RNA kissing complex. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. The Back 2 Activity Trial: education and advice versus education and advice plus a structured walking programme for chronic low back pain

    Directory of Open Access Journals (Sweden)

    Bradbury Ian

    2010-07-01

    Full Text Available Abstract Background Current evidence supports the use of exercise-based treatment for chronic low back pain that encourages the patient to assume an active role in their recovery. Walking has been shown it to be an acceptable type of exercise with a low risk of injury. However, it is not known whether structured physical activity programmes are any more effective than giving advice to remain active. Methods/Design The proposed study will test the feasibility of using a pedometer-driven walking programme, as an adjunct to a standard education and advice session in participants with chronic low back pain. Fifty adult participants will be recruited via a number of different sources. Baseline outcome measures including self reported function; objective physical activity levels; fear-avoidance beliefs and health-related quality of life will be recorded. Eligible participants will be randomly allocated under strict, double blind conditions to one of two treatments groups. Participants in group A will receive a single education and advice session with a physiotherapist based on the content of the 'Back Book'. Participants in group B will receive the same education and advice session. In addition, they will also receive a graded pedometer-driven walking programme prescribed by the physiotherapist. Follow up outcomes will be recorded by the same researcher, who will remain blinded to group allocation, at eight weeks and six months post randomisation. A qualitative exploration of participants' perception of walking will also be examined by use of focus groups at the end of the intervention. As a feasibility study, treatment effects will be represented by point estimates and confidence intervals. The assessment of participant satisfaction will be tabulated, as will adherence levels and any recorded difficulties or adverse events experienced by the participants or therapists. This information will be used to modify the planned interventions to be used in a

  2. Intimacy, Confidentiality, and Power: Kiss and Tell as a Feminist Critique of Privacy Law

    Directory of Open Access Journals (Sweden)

    Naomh Gibson

    2015-12-01

    Full Text Available The current principle of privacy and its enactment in law and policy is presented as a reified, universal value that is gender-neutral. However this article contends this presumption, and advances that privacy is an inconsistent area of law that has allowed for the oppression of women’s rights and interests. It will be proposed that the narrative of ‘kiss and tell’ stories offers access to substantive justice and equality by subverting legal and gender norms and deconstructing the concept of privacy. Using the tools of feminist legal theory and theoretical commentary, this argument forms four sections. Firstly, it will be introduced that privacy is a value which is nebulous at best, and the reasons for critiquing privacy law using a perspective from feminist legal theory will be explored. Following this, the injustices perpetrated against women by the current state of privacy law will be outlined – particularly in the area of sexual information and sexuality. Using examples from both the UK and American jurisdictions, it will be submitted that privacy law is mired in patriarchal values. Thirdly, the jurisprudence underpinning privacy law decisions on sexual relationships and the legal concepts of confidence and intimacy will be critically examined and deconstructed. Finally, drawing upon investigative research, anecdotal evidence, and critical analysis, it will be submitted that ‘kiss and tell’ narratives are the way forward to reconceptualise privacy. It will be recommended that kiss and tell stories have value in social communication and present a chance for women to engage in relevant public discourse, and more widely, gives law an opportunity to reach a new understanding of privacy appropriate in the modern age.

  3. Endovascular Management of Aorta-Iliac Stenosis and Occlusive Disease by Kissing-Stent Technique.

    Science.gov (United States)

    Liu, Meng; Zhang, Fuxian

    2016-01-01

    Kissing-stenting treatment has been used to treat patients with peripheral artery disease (PAD). However, the long term efficacy of the stenting therapy is not well defined in Chinese PAD patients. To investigate the question, sixty-three PAD patients (37 males and 26 females), aged 66 ± 7.3 years, were analysed in the study. They were featured as claudication (n = 45, 71.4%), rest pain (n = 18, 28.6%), or gangrene (n = 8, 12.7%). In total, 161 stents were applied in aorta-iliac lesions with 2.6 stents for each patient, including 55 self-expanding stents, 98 balloon expandable stents, and 8 covered stents. The success rate of implanting Kissing-stents was 100%. Catheter-directed thrombolysis (CDT) with urokinase was performed in 8 cases (12.7%). The severity of peripheral ischemia was significantly improved, as evidenced by 3.3-fold increase of ankle-brachial pressure index (ABI) after the surgery (P = 0.008). One, three, five, and seven years after surgery, the primary patency rate was 87.3%, 77.4%, 71.1%, and 65.0%, whereas the secondary patency rate was 95.2%, 92.5%, 89.5%, and 85.0%, respectively. No in-hospital mortality was recorded. In conclusion, Kissing-stenting technique for aorta-iliac lesions is safe and effective with lower complications. It is beneficial for aorta-iliac occlusions that are longer than 60 mm.

  4. Endovascular Management of Aorta-Iliac Stenosis and Occlusive Disease by Kissing-Stent Technique

    Directory of Open Access Journals (Sweden)

    Meng Liu

    2016-01-01

    Full Text Available Kissing-stenting treatment has been used to treat patients with peripheral artery disease (PAD. However, the long term efficacy of the stenting therapy is not well defined in Chinese PAD patients. To investigate the question, sixty-three PAD patients (37 males and 26 females, aged 66±7.3 years, were analysed in the study. They were featured as claudication (n=45, 71.4%, rest pain (n=18, 28.6%, or gangrene (n=8, 12.7%. In total, 161 stents were applied in aorta-iliac lesions with 2.6 stents for each patient, including 55 self-expanding stents, 98 balloon expandable stents, and 8 covered stents. The success rate of implanting Kissing-stents was 100%. Catheter-directed thrombolysis (CDT with urokinase was performed in 8 cases (12.7%. The severity of peripheral ischemia was significantly improved, as evidenced by 3.3-fold increase of ankle-brachial pressure index (ABI after the surgery (P=0.008. One, three, five, and seven years after surgery, the primary patency rate was 87.3%, 77.4%, 71.1%, and 65.0%, whereas the secondary patency rate was 95.2%, 92.5%, 89.5%, and 85.0%, respectively. No in-hospital mortality was recorded. In conclusion, Kissing-stenting technique for aorta-iliac lesions is safe and effective with lower complications. It is beneficial for aorta-iliac occlusions that are longer than 60 mm.

  5. Similarities between intra- and intermolecular hydrogen bonds in RNA kissing complexes found by means of cross-correlated relaxation

    Energy Technology Data Exchange (ETDEWEB)

    Dittmer, Jens [Institut de Chimie Moleculaire et Biologique, Ecole Polytechnique Federale de Lausanne, BCH (Switzerland); Kim, Chul-Hyun [University of California, Department of Chemistry (United States); Bodenhausen, Geoffrey [Institut de Chimie Moleculaire et Biologique, Ecole Polytechnique Federale de Lausanne, BCH (Switzerland)], E-mail: Geoffrey.Bodenhausen@ens.fr

    2003-07-15

    The bond lengths and dynamics of intra- and intermolecular hydrogen bonds in an RNA kissing complex have been characterized by determining the NMR relaxation rates of various double- and triple-quantum coherences that involve an imino proton and two neighboring nitrogen-15 nuclei belonging to opposite bases. New experiments allow one to determine the chemical shift anisotropy of the imino protons. The bond lengths derived from dipolar relaxation and the lack of modulations of the nitrogen chemical shifts indicate that the intermolecular hydrogen bonds which hold the kissing complex together are very similar to the intramolecular hydrogen bonds in the double-stranded stem of the RNA.

  6. Bioergia Research Programme

    Energy Technology Data Exchange (ETDEWEB)

    Asplund, D.

    1997-12-31

    The main objectives of Finland`s Bioenergia Research Programme are (1) To develop new methods of producing biofuels which can compete with imported fuels, demonstrating the most promising production methods through pilot schemes, (2) To develop and demonstrate 3 - 4 new pieces of equipment or methods connected with handling and using bioenergy, (3) To produce basic information on conversion techniques and evaluate the quality, usability and environmental impacts of the products as well as the overall economy of the entire production chain and to create 2-3 conversion methods for follow-up development by industry. The principle research areas are (1) Development of production technology for wood-derived fuels, (2) Peat production, (3) The use of bioenergy and (4) Biomass conversion. This conference paper discusses the results obtained so far and reviews in some detail the activities of the programme. 3 figs., 3 tabs.

  7. What does determine gonad weight in the wild kissing bug Mepraia spinolai

    Directory of Open Access Journals (Sweden)

    Carezza Botto-Mahan

    2007-03-01

    Full Text Available Female fecundity increases with body size in a variety of insects, but it is unknown if this generalization applies for kissing bugs. In this study, we evaluate whether gonad weight in the bloodsucking insect Mepraia spinolai correlates with body size, or determined by nutrition or developmental time. We found that the investment on reproductive tissue correlates positively and significantly with body size and with the amount of ingested blood by female insects along their lifespan. Total molting time did not significantly affect gonad weight. We suggest that under optimal feeding conditions M. spinolai females could express their maximum reproductive potential.

  8. Simultaneous kissing stent in a patient with severe bifurcation pulmonary vein stenosis.

    Science.gov (United States)

    Cubeddu, Roberto J; Gulati, Vishal K

    2015-02-01

    Pulmonary vein stenosis (PVS) is a late and rare complication of pulmonary vein isolation for the treatment of atrial fibrillation. The ideal approach to the management of PVS has not yet been established, however, corrective procedures may include both surgical and percutaneous techniques. We describe the case of a complex bifurcation lesion involving the left superior pulmonary vein. The condition required percutaneous intervention using a modified kissing stent technique with bare metal stents that resulted in an excellent post-operative course, sustained symptomatic relief, and uncomplicated 1-year follow-up. © 2014 Wiley Periodicals, Inc. © 2014 Wiley Periodicals, Inc.

  9. Endovascular Treatment of Blunt Traumatic Abdominal Aortic Occlusion With Kissing Stent Placement

    Energy Technology Data Exchange (ETDEWEB)

    Idoguchi, Koji, E-mail: idoguchi@ares.eonet.ne.jp; Yamaguchi, Masato; Okada, Takuya [Kobe University Hospital, Department of Radiology and Center for Endovascular Therapy (Japan); Nomura, Yoshikatsu [Kobe University Hospital, Department of Cardiovascular Surgery (Japan); Sugimura, Kazuro [Kobe University Hospital, Department of Radiology and Center for Endovascular Therapy (Japan); Okita, Yutaka [Kobe University Hospital, Department of Cardiovascular Surgery (Japan); Sugimoto, Koji [Kobe University Hospital, Department of Radiology and Center for Endovascular Therapy (Japan)

    2012-10-15

    Blunt traumatic abdominal aortic dissection is extremely rare and potentially deadly. We present the case of a 62-year-old man involved in a frontal car crash. After emergency undergoing laparotomy for bowel injuries, he was referred to our hospital due to acute ischemia of bilateral lower extremities on day 3 after the trauma. Computed tomography and aortography showed an aortobiiliac dissection with complete occlusion. This injury was successfully treated by endovascular treatment with 'kissing'-technique stent placement, which appears to be a safe, effective, and minimally invasive treatment.

  10. Technical proposal for including health in the procedures for assessing the environmental impact of policies, plans, programmes, projects and activities

    Directory of Open Access Journals (Sweden)

    Almudena García Nieto

    2015-05-01

    Full Text Available Health was an element of general licensing procedures until Spain joined the EU in 1986, when the health report became diluted. The purpose of this article is to provide an overview of this topic’s current regulatory framework and to try to briefly describe health priorities and the channels for feasibly integrating the health variable in the environmental assessment of plans, programmes and projects from the public and private sectors. The current existence of the Environmental Assessment Act and the Public Health Act may help to achieve this.When preparing a strategic environmental study and an environmental impact study, the health impact assessment should be considered an essential step in these environmental procedures and have the same legal treatment as the “compulsory and determinant reports” of said procedures.Thus, it is concluded that the regulatory development of the aspects relating to the assessment of the health impact of the plans, programmes and projects envisaged in the Environmental Assessment Act is essential, the health impact assessment being the tool for doing so.

  11. The HAT TRICK programme for improving physical activity, healthy eating and connectedness among overweight, inactive men: study protocol of a pragmatic feasibility trial.

    Science.gov (United States)

    Caperchione, Cristina M; Bottorff, Joan L; Oliffe, John L; Johnson, Steven T; Hunt, Kate; Sharp, Paul; Fitzpatrick, Kayla M; Price, Ryley; Goldenberg, S Larry

    2017-09-06

    Physical activity, healthy eating and maintaining a healthy weight are associated with reduced risk of cardiovascular disease, type 2 diabetes and cancer and with improved mental health. Despite these benefits, many men do not meet recommended physical activity guidelines and have poor eating behaviours. Many health promotion programmes hold little appeal to men and consequently fail to influence men's health practices. HAT TRICK was designed as a 12-week face-to-face, gender-sensitised intervention for overweight and inactive men focusing on physical activity, healthy eating and social connectedness and was delivered in collaboration with a major junior Canadian ice hockey team (age range 16-20 years). The programme was implemented and evaluated to assess its feasibility. This article describes the intervention design and study protocol of HAT TRICK. HAT TRICK participants (n=60) were men age 35 years, residing in the Okanagan Region of British Columbia, who accumulate 150 min of moderate to vigorous physical activity a week, with a body mass index of >25 kg/m(2) and a pant waist size of >38'. Each 90 min weekly session included targeted health education and theory-guided behavioural change techniques, as well as a progressive (ie, an increase in duration and intensity) group physical activity component. Outcome measures were collected at baseline, 12 weeks and 9 months and included the following: objectively measured anthropometrics, blood pressure, heart rate, physical activity and sedentary behaviour, as well as self-reported physical activity, sedentary behaviour, diet, smoking, alcohol consumption, sleep habits, risk of depression, health-related quality of life and social connectedness. Programme feasibility data (eg, recruitment, satisfaction, adherence, content delivery) were assessed at 12 weeks via interviews and self-report. Ethical approval was obtained from the University of British Columbia Okanagan Behavioural Research Ethics Board (reference no H

  12. Expression of KISS-1 in human breast cancer tissue and its clinical significance%KISS-1基因在乳腺癌组织中的表达及临床意义

    Institute of Scientific and Technical Information of China (English)

    吴爱国; 张华霖; 陈海金; 刘鹏

    2006-01-01

    目的 研究KISS-1基因在人乳腺癌组织表达及其与转移的关系.方法 采用逆转录聚合酶链式扩增反应和免疫组化技术对56例人乳腺癌组织中KISS-1 mRNA及其蛋白metastin的表达进行检测.结果 乳腺癌组织的KISS-1 mRNA及metastin阳性表达率均显著低于正常乳腺组织(P=0.000),且与乳腺癌的淋巴结转移显著相关(P<0.01);而与乳腺癌的肿块大小、组织学分级等临床病理因素无关(P>0.05).结论 KISS-1基因表达降低与人乳腺癌转移密切相关,可能参与人乳腺癌转移的调控.

  13. Randomized Comparison of Final Kissing Balloon Dilatation Versus No Final Kissing Balloon Dilatation in Patients With Coronary Bifurcation Lesions Treated With Main Vessel Stenting. Five Year Clinical Outcome in The Nordic-Baltic Bifurcation Study III

    DEFF Research Database (Denmark)

    Niemelä, Matti; Holm, Niels R; Kervinen, Kari

    2015-01-01

    Background- It is unknown whether the preferred 1-stent bifurcation stenting approach with stenting of the main vessel (MV) and optional side branch stenting using drug-eluting stents should be finalized by a kissing balloon dilatation (FKBD). Therefore, we compared strategies of MV stenting with...

  14. Randomized Comparison of Final Kissing Balloon Dilatation Versus No Final Kissing Balloon Dilatation in Patients With Coronary Bifurcation Lesions Treated With Main Vessel Stenting: The Nordic-Baltic Bifurcation Study III

    DEFF Research Database (Denmark)

    Niemelä, Matti; Kervinen, Kari; Erglis, Andrejs

    2010-01-01

    Background- It is unknown whether the preferred 1-stent bifurcation stenting approach with stenting of the main vessel (MV) and optional side branch stenting using drug-eluting stents should be finalized by a kissing balloon dilatation (FKBD). Therefore, we compared strategies of MV stenting...

  15. ATIVISMO DE VÍTIMAS DO INCÊNDIO NA BOATE KISS: EVENTO TRAUMÁTICO, CAUSA PÚBLICA E CONFLITOS MORAIS

    Directory of Open Access Journals (Sweden)

    Ana Paula Arosi

    2017-01-01

    Full Text Available This article addresses the activism of victims’ relatives of the Kiss nightclub fire that took place in Santa Maria, in south Brazil, in 2013 causing 242 deaths. I have been accompanying for the past three years the actions carried out by the Santa Maria do Luto à Luta movement. I focus here on the relationship between this movement and the government, analyzing the public health policies and the conflict emerged since the incident between the movement and the Ministério Público, a government institution which is responsible for watching over diffuse and individual rights in its own local sphere. What this conflict points out is the fact that the State’s omission enhances the perception of the event as traumatic by those who have been affected by it. My research converses with several other anthropological works that also focus, in some way or another, on the relationship between victims’ movements and the State in order to emphasize the connection between suffering, the State’s neglect and the construction of a cause. In my own work, I take notice of some forms of legitimation of the victim’s discourse, created by family members as they fight the derrogatory claims of “madness” thrust upon them. Finally, I take a look on the broader debates about the victim’s place in public space and the language of suffering as a way of positioning oneself politically. The relationship with the State also offers some insights regarding the production of the subjectivities of those involved with the Kiss event; it is through this production, precisely, that the event will be interpreted.

  16. 非小细胞肺癌中Kiss-1的表达和淋巴管生成的意义%Significance of Kiss-1 Expression and Lymphangiogenesis with Non-Small Cell Lung Cancer

    Institute of Scientific and Technical Information of China (English)

    王祖义; 黎传奎; 王萍; 刘学刚; 唐震; 汪国文

    2009-01-01

    目的 探讨非小细胞肺癌(Non-Small Cell Lung Cancer,NSCLC)转移抑制基因Kiss-1表达下调或缺失和淋巴管生成与淋巴结转移是否具有协同作用.方法 兔抗人单克隆抗体Kiss-1蛋白metastin(45-54)-NH2和抗Podoplanin单克隆抗体(Elivision法)对68例NSCLC术后石蜡切片进行染色,显微镜下观察Kiss-1的表达和肿瘤间质及癌旁组织淋巴管生成情况.结果 ①NSCLC组织内淋巴结阳性组Kiss-1的表达阳性率为39.4%(13/33),淋巴结阴性组Kiss-1的表达阳性率为91.4%(32/35),两组相比差异有统计学意义(P<0.05);②肿瘤组织间质中淋巴结阳性患者淋巴管密度(Lymphatic vessel density,LVD)平均值为15.73±0.74,淋巴结阴性患者LVD平均值为8.31±0.32,二者相比差异有统计学意义(P<0.05).③logistic回归分析显示,仅LVD对淋巴结转移有影响(P<0.05),其相对危险度(RR)为2.065,95%可信区间(1.202,3.547).而Kiss-1的表达下调无意义,P值为0.569,95%可信区间为(0.011,12.143).二者之间对淋巴结转移无明显协同作用(P<0.05).结论 转移抑制基因Kiss-1的表达下调和淋巴管生成对淋巴结转移未见明显协同作用.

  17. Serine protease inhibitor 6 is required to protect dendritic cells from the kiss of death.

    Science.gov (United States)

    Lovo, Elena; Zhang, Manling; Wang, Lihui; Ashton-Rickardt, Philip G

    2012-02-01

    How dendritic cells (DC) present Ag to cytotoxic T cells (CTL) without themselves being killed through contact-mediated cytotoxicity (so-called kiss of death) has proved to be controversial. Using mice deficient in serine protease inhibitor 6 (Spi6), we show that Spi6 protects DC from the kiss of death by inhibiting granzyme B (GrB) delivered by CTL. Infection of Spi6 knockout mice with lymphocytic choriomeningitis virus revealed impaired survival of CD8α DC. The impaired survival of Spi6 knockout CD8α DC resulted in impaired priming and expansion of both primary and memory lymphocytic choriomeningitis virus-specific CTL, which could be corrected by GrB deficiency. The rescue in the clonal burst obtained by GrB elimination demonstrated that GrB was the physiological target through which Spi6 protected DC from CTL. We conclude that the negative regulation of DC priming of CD8 T lymphocyte immunity by CTL killing is mitigated by the physiological inhibition of GrB by Spi6.

  18. How music alters a kiss: superior temporal gyrus controls fusiform–amygdalar effective connectivity

    Science.gov (United States)

    Deserno, Lorenz; Bakels, Jan-Hendrik; Schlochtermeier, Lorna H.; Kappelhoff, Hermann; Jacobs, Arthur M.; Fritz, Thomas Hans; Koelsch, Stefan; Kuchinke, Lars

    2014-01-01

    While watching movies, the brain integrates the visual information and the musical soundtrack into a coherent percept. Multisensory integration can lead to emotion elicitation on which soundtrack valences may have a modulatory impact. Here, dynamic kissing scenes from romantic comedies were presented to 22 participants (13 females) during functional magnetic resonance imaging scanning. The kissing scenes were either accompanied by happy music, sad music or no music. Evidence from cross-modal studies motivated a predefined three-region network for multisensory integration of emotion, consisting of fusiform gyrus (FG), amygdala (AMY) and anterior superior temporal gyrus (aSTG). The interactions in this network were investigated using dynamic causal models of effective connectivity. This revealed bilinear modulations by happy and sad music with suppression effects on the connectivity from FG and AMY to aSTG. Non-linear dynamic causal modeling showed a suppressive gating effect of aSTG on fusiform–amygdalar connectivity. In conclusion, fusiform to amygdala coupling strength is modulated via feedback through aSTG as region for multisensory integration of emotional material. This mechanism was emotion-specific and more pronounced for sad music. Therefore, soundtrack valences may modulate emotion elicitation in movies by differentially changing preprocessed visual information to the amygdala. PMID:24298171

  19. How music alters a kiss: superior temporal gyrus controls fusiform-amygdalar effective connectivity.

    Science.gov (United States)

    Pehrs, Corinna; Deserno, Lorenz; Bakels, Jan-Hendrik; Schlochtermeier, Lorna H; Kappelhoff, Hermann; Jacobs, Arthur M; Fritz, Thomas Hans; Koelsch, Stefan; Kuchinke, Lars

    2014-11-01

    While watching movies, the brain integrates the visual information and the musical soundtrack into a coherent percept. Multisensory integration can lead to emotion elicitation on which soundtrack valences may have a modulatory impact. Here, dynamic kissing scenes from romantic comedies were presented to 22 participants (13 females) during functional magnetic resonance imaging scanning. The kissing scenes were either accompanied by happy music, sad music or no music. Evidence from cross-modal studies motivated a predefined three-region network for multisensory integration of emotion, consisting of fusiform gyrus (FG), amygdala (AMY) and anterior superior temporal gyrus (aSTG). The interactions in this network were investigated using dynamic causal models of effective connectivity. This revealed bilinear modulations by happy and sad music with suppression effects on the connectivity from FG and AMY to aSTG. Non-linear dynamic causal modeling showed a suppressive gating effect of aSTG on fusiform-amygdalar connectivity. In conclusion, fusiform to amygdala coupling strength is modulated via feedback through aSTG as region for multisensory integration of emotional material. This mechanism was emotion-specific and more pronounced for sad music. Therefore, soundtrack valences may modulate emotion elicitation in movies by differentially changing preprocessed visual information to the amygdala.

  20. Valve-Like and Protruding Calcified Intimal Flap Complicating Common Iliac Arteries Kissing Stenting

    Directory of Open Access Journals (Sweden)

    George S. Georgiadis

    2015-01-01

    Full Text Available Endovascular therapy for iliac artery chronic total occlusions is nowadays associated with low rates of procedure-related complications and improved clinical outcomes, and it is predominantly used as first-line therapy prior to aortobifemoral bypass grafting. Herein, we describe the case of a patient presenting with an ischemic left foot digit ulcer and suffering complex aortoiliac lesions, who received common iliac arteries kissing stents, illustrating at final antegrade and retrograde angiograms the early recognition of a blood flow obstructing valve-like calcified intimal flap protruding through the stent struts, which was obstructing antegrade but not retrograde unilateral iliac arterial axis blood flow. The problem was resolved by reconstructing the aortic bifurcation at a more proximal level. Completion angiogram verified normal patency of aorta and iliac vessels. Additionally, a severe left femoral bifurcation stenosis was also corrected by endarterectomy-arterioplasty with a bovine patch. Postintervention ankle brachial pressure indices were significantly improved. At the 6-month and 2-year follow-up, normal peripheral pulses were still reported without intermittent claudication suggesting the durability of the procedure. Through stent-protruding calcified intimal flap, is a very rare, but existing source of antegrade blood flow obstruction after common iliac arteries kissing stents.

  1. Structure-Function Model for Kissing Loop Interactions That Initiate Dimerization of Ty1 RNA

    Directory of Open Access Journals (Sweden)

    Eric R. Gamache

    2017-04-01

    Full Text Available The genomic RNA of the retrotransposon Ty1 is packaged as a dimer into virus-like particles. The 5′ terminus of Ty1 RNA harbors cis-acting sequences required for translation initiation, packaging and initiation of reverse transcription (TIPIRT. To identify RNA motifs involved in dimerization and packaging, a structural model of the TIPIRT domain in vitro was developed from single-nucleotide resolution RNA structural data. In general agreement with previous models, the first 326 nucleotides of Ty1 RNA form a pseudoknot with a 7-bp stem (S1, a 1-nucleotide interhelical loop and an 8-bp stem (S2 that delineate two long, structured loops. Nucleotide substitutions that disrupt either pseudoknot stem greatly reduced helper-Ty1-mediated retrotransposition of a mini-Ty1, but only mutations in S2 destabilized mini-Ty1 RNA in cis and helper-Ty1 RNA in trans. Nested in different loops of the pseudoknot are two hairpins with complementary 7-nucleotide motifs at their apices. Nucleotide substitutions in either motif also reduced retrotransposition and destabilized mini- and helper-Ty1 RNA. Compensatory mutations that restore base-pairing in the S2 stem or between the hairpins rescued retrotransposition and RNA stability in cis and trans. These data inform a model whereby a Ty1 RNA kissing complex with two intermolecular kissing-loop interactions initiates dimerization and packaging.

  2. Structure-Function Model for Kissing Loop Interactions That Initiate Dimerization of Ty1 RNA.

    Science.gov (United States)

    Gamache, Eric R; Doh, Jung H; Ritz, Justin; Laederach, Alain; Bellaousov, Stanislav; Mathews, David H; Curcio, M Joan

    2017-04-26

    The genomic RNA of the retrotransposon Ty1 is packaged as a dimer into virus-like particles. The 5' terminus of Ty1 RNA harbors cis-acting sequences required for translation initiation, packaging and initiation of reverse transcription (TIPIRT). To identify RNA motifs involved in dimerization and packaging, a structural model of the TIPIRT domain in vitro was developed from single-nucleotide resolution RNA structural data. In general agreement with previous models, the first 326 nucleotides of Ty1 RNA form a pseudoknot with a 7-bp stem (S1), a 1-nucleotide interhelical loop and an 8-bp stem (S2) that delineate two long, structured loops. Nucleotide substitutions that disrupt either pseudoknot stem greatly reduced helper-Ty1-mediated retrotransposition of a mini-Ty1, but only mutations in S2 destabilized mini-Ty1 RNA in cis and helper-Ty1 RNA in trans. Nested in different loops of the pseudoknot are two hairpins with complementary 7-nucleotide motifs at their apices. Nucleotide substitutions in either motif also reduced retrotransposition and destabilized mini- and helper-Ty1 RNA. Compensatory mutations that restore base-pairing in the S2 stem or between the hairpins rescued retrotransposition and RNA stability in cis and trans. These data inform a model whereby a Ty1 RNA kissing complex with two intermolecular kissing-loop interactions initiates dimerization and packaging.

  3. Endovascular reconstruction of iliac artery bifurcation atherosclerotic disease with the kissing technique.

    Science.gov (United States)

    Vourliotakis, Georgios; Mantas, Georgios; Katsargyris, Athanasios; Aivatidi, Christine; Kandounakis, Yannis

    2013-10-01

    A 71-year-old male patient with severe left buttock and lower-extremity claudication due to iliac artery bifurcation stenoses was referred to our institution for endovascular treatment. A 'kissing' technique was used in order to dilate the proximal parts of both internal and external iliac arteries and avoid compromization of the internal iliac artery during proximal external iliac artery stenting. A balloon expandable stent was inserted via a left ipsilateral retrograde access to the narrowed origin of the left external iliacartery and a balloon catheter via a right contralateral access inside the origin of the left internal iliac artery. Simultaneous balloons inflation restored full patency of both vessels. Twelve months later the patient is doing well, free of buttock or lower-extremity claudication symptoms. For iliac artery bifurcation atherosclerotic disease, endovascular repair with the 'kissing' technique can achieve a complete bifurcation reconstruction offering significant clinical benefit in selected patients. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  4. Can Population-Level Laterality Stem from Social Pressures? Evidence from Cheek Kissing in Humans.

    Science.gov (United States)

    Chapelain, Amandine; Pimbert, Pauline; Aube, Lydiane; Perrocheau, Océane; Debunne, Gilles; Bellido, Alain; Blois-Heulin, Catherine

    2015-01-01

    Despite extensive research, the origins and functions of behavioural laterality remain largely unclear. One of the most striking unresolved issues is the fact that laterality generally occurs at the population-level. Why would the majority of the individuals of a population exhibit the same laterality, while individual-level laterality would yet provide the advantages in terms of improving behavioural efficiency? Are social pressures the key factor? Can social pressures induce alignment of laterality between the individuals of a population? Can the effect of social pressures overpass the effect of other possible determining factors (e.g. genes)? We tested this important new hypothesis in humans, for the first time. We asked whether population-level laterality could stem from social pressures. Namely, we assessed social pressures on laterality in an interactive social behaviour: kissing on the cheek as a greeting. We performed observations in 10 cities of France. The observations took place in spots where people of the city meet and greet each other. We showed that: a) there is a population-level laterality for cheek kissing, with the majority of individuals being aligned in each city, and b) there is a variation between populations, with a laterality that depends on the city. These results were confirmed by our complementary data from questionnaires and internet surveys. These findings show that social pressures are involved in determining laterality. They demonstrate that population-level laterality can stem from social pressures.

  5. Development of a nonlinear ultrasonic NDE technique for detection of kissing bonds in composites

    Science.gov (United States)

    Alston, Jonathan; Croxford, Anthony; Potter, Jack; Blanloeuil, Philippe

    2017-04-01

    The development of low-cost bonded assembly of composite aerospace structures ideally requires an NDE method to detect the presence of poor quality, weak bonds or kissing bonds. Such interfaces can introduce nonlinearity as a result of contact nonlinearity where an ultrasonic wave is distorted when it interacts with the interface. In general, the nonlinear elastic behaviour of these interfaces will generate harmonics but they can be lost among the harmonics generated by other nonlinearities present in the experimental system. The technique developed in this research is a non-collinear method; this involves the interaction of two ultrasonic beams, and it allows the removal of virtually all system nonlinearity except for that produced in the region where the two beams overlap. The frequencies of the two beams and the angle between are varied during the experiment. By measuring the nonlinear mixing response as these two parameters are swept through a `fingerprint' of the nonlinear properties in the interaction region can be obtained. This fingerprint has been shown to contain information about the bulk material and the interface status. Work is ongoing to understand which features in the fingerprints reliably correlate with particular material or interface properties. To build this understanding a greatly simplified kissing bond, a compression loaded aluminium-aluminium interface, has been tested. Modelling of the nonlinear behaviour of the aluminium interface has also been conducted.

  6. Expression level and significance of Kiss-1 and Cox-2 in primary hepatocellular carcinoma%Kiss-1和环氧化酶-2在原发性肝癌中的表达及意义

    Institute of Scientific and Technical Information of China (English)

    任连伟; 刘超; 庞志刚; 王广田; 尚闯; 王彬

    2011-01-01

    目的 研究环氧化酶-2(Cox-2) RNA和Kiss-1 RNA在原发性肝细胞肝癌中的表达及临床意义.方法采用RT-PCR技术检测Cox-2 RNA和Kiss-1 RNA在正常肝组织(10例)、癌旁肝硬化组织(30例)和肝癌组织(39例)的表达情况,并比较它们在不同组织中表达的差异及临床意义.结果 Cox-2 RNA在正常的肝组织中不表达,在肝硬化组和肝癌组中高表达,但两者比较差异无统计学意义(P>0.05),但都与正常组比较差异有统计学意义(P0.05).Kiss-1 RNA在正常肝组织,肝硬化和肝癌组织中均有表达,正常组和肝硬化组比较差异无统计学意义(P>0.05),但都与肝癌组比较差异有统计学意义(P0.05).结论 Cox-2 RNA在肝癌中表达明显升高,实验证明它可能与肿瘤的浸润、转移等无关;Kiss-1 RNA的表达在肝癌中抑制显著,实验证明它可能与肿瘤的浸润、转移等有密切相关.同时Cox-2 RNA和Kiss-1 RNA的表达呈显著负相关(P0.05), but have significantly higher expression compared to normal group (P0.05). Kiss-1 RNA expression had a high expression in normal tissues, cirrhosis tissues and HCC tissues, Kiss-1 RNA expression in HCC tissues was significantly lower than that in normal tissues and cirrhosis tissues(P0.05).The expression of Kiss-1RNA in HCC tissues was reduced and had significant relation with TNM stage and Edmenson grade of tumor (P0.05). Conclusions The expression of Cox-2 RNA is very high in HCC tissues maybe have no relation with invasion and metastasis;Kiss-1 RNA expression is obvious suppressed in HCC tissues,the reduced kiss-1 RNA expression maybe helpful to the invasion and metastasis of tumor. A close negative correlation between the both genes was showed(P<0.05).

  7. Polymorphisms rs12998 and rs5780218 in KiSS1 Suppressor Metastasis Gene in Mexican Patients with Breast Cancer

    Directory of Open Access Journals (Sweden)

    Edhit Guadalupe Cruz Quevedo

    2015-01-01

    Full Text Available Aims. KiSS1 is a metastasis suppressor gene associated with inhibition of cellular chemotaxis and invasion attenuating the metastasis in melanoma and breast cancer cell lines. Along the KiSS-1 gene at least 294 SNPs have been described; however the association of these polymorphisms as genetic markers for metastasis in breast cancer studies has not been investigated. Here we describe two simple PCR-RFLPs protocols to identify the rs5780218 (9DelT and the rs12998 (E20K KiSS1 polymorphisms and the allelic, genotypic, and haplotypic frequencies in Mexican general population (GP and patients with benign breast disease (BBD or breast cancer (BC. Results. The rs5780218 polymorphism was individually associated with breast cancer (P=0.0332 and the rs12998 polymorphism shows statistically significant differences when GP versus case (BC and BBD groups were compared (P<0.0001. The H1 Haplotype (G/- occurred more frequently in BC group (0.4256 whereas H2 haplotype (G/T was the most prevalent in BBD group (0.4674. Conclusions. Our data indicated that the rs5780218 polymorphism individually confers susceptibility for development of breast cancer in Mexican population and a possible role as a genetic marker in breast cancer metastasis for H1 haplotype (Wt/variant in KiSS1 gene must be analyzed in other populations.

  8. THE KISSING BALLOON TECHNIQUE WITH 2 OVER-THE-WIRE BALLOON CATHETERS THROUGH A SINGLE 8-FRENCH GUIDING CATHETER

    NARCIS (Netherlands)

    DENHEIJER, P; BERNINK, PJLM; VANDIJK, RB; TWISK, SPM; LIE, KI

    1991-01-01

    Some of the newer over-the-wire coronary angioplasty catheters have shaft sizes of 3.0 French (F) or less. The inner diameter of modern 8-F guiding catheters is large enough to accommodate two of such balloon catheters. We report a kissing balloon procedure with two over-the-wire catheters through a

  9. In vivo geometry of the kissing stent and covered endovascular reconstruction of the aortic bifurcation configurations in aortoiliac occlusive disease

    NARCIS (Netherlands)

    Groot Jebbink, Erik; Ter Mors, Thijs G; Slump, Cornelis H; Geelkerken, Robert H; Holewijn, Suzanne; Reijnen, Michel MPJ

    2017-01-01

    Objectives Various configurations of kissing stent (KS) configurations exist and patency rates vary. In response the covered endovascular reconstruction of the aortic bifurcation configuration was designed to minimize mismatch and improve outcome. The aim of the current study is to compare

  10. From Kissing to Coitus? Sex-of-Partner Differences in the Sexual Milestone Achievement of Young Men

    Science.gov (United States)

    Smiler, Andrew P.; Frankel, Loren B. W.; Savin-Williams, Ritch C.

    2011-01-01

    Scientific information regarding normative patterns of young men's sexual behavior is insufficient, especially regarding the impact of sex of partner. We explored the age at which 255 young adult men achieved several milestones (e.g., first kiss, manual-genital contact, intercourse) as well as the sequence of milestone achievement and stability in…

  11. THE KISSING BALLOON TECHNIQUE WITH 2 OVER-THE-WIRE BALLOON CATHETERS THROUGH A SINGLE 8-FRENCH GUIDING CATHETER

    NARCIS (Netherlands)

    DENHEIJER, P; BERNINK, PJLM; VANDIJK, RB; TWISK, SPM; LIE, KI

    Some of the newer over-the-wire coronary angioplasty catheters have shaft sizes of 3.0 French (F) or less. The inner diameter of modern 8-F guiding catheters is large enough to accommodate two of such balloon catheters. We report a kissing balloon procedure with two over-the-wire catheters through a

  12. The (cost-effectiveness of an individually tailored long-term worksite health promotion programme on physical activity and nutrition: design of a pragmatic cluster randomised controlled trial

    Directory of Open Access Journals (Sweden)

    Burdorf Alex

    2007-09-01

    Full Text Available Abstract Background Cardiovascular disease is the leading cause of disability and mortality in most Western countries. The prevalence of several risk factors, most notably low physical activity and poor nutrition, is very high. Therefore, lifestyle behaviour changes are of great importance. The worksite offers an efficient structure to reach large groups and to make use of a natural social network. This study investigates a worksite health promotion programme with individually tailored advice in physical activity and nutrition and individual counselling to increase compliance with lifestyle recommendations and sustainability of a healthy lifestyle. Methods/Design The study is a pragmatic cluster randomised controlled trial with the worksite as the unit of randomisation. All workers will receive a standard worksite health promotion program. Additionally, the intervention group will receive access to an individual Health Portal consisting of four critical features: a computer-tailored advice, a monitoring function, a personal coach, and opportunities to contact professionals at request. Participants are employees working for companies in the Netherlands, being literate enough to read and understand simple Internet-based messages in the Dutch language. A questionnaire to assess primary outcomes (compliance with national recommendations on physical activity and on fruit and vegetable intake will take place at baseline and after 12 and 24 months. This questionnaire also assesses secondary outcomes including fat intake, self-efficacy and self-perceived barriers on physical activity and fruit and vegetable intake. Other secondary outcomes, including a cardiovascular risk profile and physical fitness, will be measured at baseline and after 24 months. Apart from the effect evaluation, a process evaluation will be carried out to gain insight into participation and adherence to the worksite health promotion programme. A cost-effectiveness analysis and

  13. Mechanism of enhanced mechanical stability of a minimal RNA kissing complex elucidated by nonequilibrium molecular dynamics simulations.

    Science.gov (United States)

    Chen, Alan A; García, Angel E

    2012-06-12

    An RNA kissing loop from the Moloney murine leukemia virus (MMLV) exhibits unusual mechanical stability despite having only two intermolecular base pairs. Mutations at this junction have been shown to destabilize genome dimerization, with concomitant reductions in viral packaging efficiency and infectivity. Optical tweezers experiments have shown that it requires as much force to break the MMLV kissing-loop complex as is required to unfold an 11-bp RNA hairpin [Li PTX, Bustamante C, Tinoco I (2006) Proc Natl Acad Sci USA 103:15847-15852]. Using nonequilibrium all-atom molecular dynamics simulations, we have developed a detailed model for the kinetic intermediates of the force-induced dissociation of the MMLV dimerization initiation site kissing loop. Two hundred and eight dissociation events were simulated (approximately 16 μs total simulation time) under conditions of constant applied external force, which we use to construct a Markov state model for kissing-loop dissociation. We find that the complex undergoes a conformational rearrangement, which allows for equal distribution of the applied force among all of the intermolecular hydrogen bonds, which is intrinsically more stable than the sequential unzipping of an ordinary hairpin. Stacking interactions with adjacent, unpaired loop adenines further stabilize the complex by increasing the repair rate of partially broken H-bonds. These stacking interactions are prominently featured in the transition state, which requires additional coordinates orthogonal to the end-to-end extension to be uniquely identified. We propose that these stabilizing features explain the unusual stability of other retroviral kissing-loop complexes such as the HIV dimerization site.

  14. An novel analog programmable power supply for active gain control of the Multi-Pixel Photon Counter (MPPC)

    CERN Document Server

    Li, Zhengwei; Xu, Yupeng; Yan, Bo; Li, Yanguo; Lu, Xuefeng; Li, Xufang; Zhang, Shuo; Chang, Zhi; Li, Jicheng; Zhang, Yifei; Zhao, Jianling

    2016-01-01

    Silicon Photo-Multipliers (SiPM) are regarded as novel photo-detector to replace conventional Photo-Multiplier Tubes (PMTs). However, the breakdown voltage dependence on the ambient temperature results in a gain variation of $\\sim$3$\\% /^{\\circ} \\mathrm C$. This can severely limit the application of this device in experiments with wide range of operating temperature, especially in space telescope. An experimental setup in dark condition was established to investigate the temperature and bias voltage dependence of gain for the Multi-Pixel Photon Counter (MPPC), one type of the SiPM developed by Hamamatsu. The gain and breakdown voltage dependence on operating temperature of an MPPC can be approximated by a linear function, which is similar to the behavior of a zener diode. The measured temperature coefficient of the breakdown voltage is $(59.4 \\pm 0.4$ mV)$/^{\\circ} \\mathrm C$. According to this fact, a programmable power supply based on two zener diodes and an operational amplifier was designed with a positiv...

  15. Evaluation of support for non-agricultural activities in Slovakia in the period 2007-2013 through the Rural Development Programme 2007-2013

    Directory of Open Access Journals (Sweden)

    Zuzana BOHATOVA

    2016-06-01

    Full Text Available Rural Development Programme of the SR (RDP SR 2007-2013 represented a comprehensive programme document for funding from the European Agricultural Fund for Rural Development (EAFRD in the programming period 2007-2013. The RDP SR 2007-2013 supported many activities related to agriculture, forestry industry, livestock production, tourism and agro-tourism. Following goals of the EAFRD were supported by the RDP SR 2007-2013: Increasing the competitiveness of agriculture, food and forestry sector; Improving the environment and landscape; Improvement of life in rural areas and Diversification of the rural economy. The last mentioned activity: Diversification of the rural economy was supported in the previous programming period by the Axis 3: Quality of life in rural areas, Measure 3.1. Diversification into non-agricultural activities. The objective of this paper is to evaluate the implementation of the Measure 3.1. The paper focuses on content evaluation of supported projects and spatial and financial allocation of request for non-repayable financial contribution. The first part of the paper is focused on the submission of request for non-repayable financial contribution: call for proposals, supported documents available for applicants, preparation of projects and their submission. The second part of the paper is oriented on evaluation of submitted projects by the Agricultural Paying Agency and payments for beneficiaries. The third part of the paper is oriented on evaluation of realization of projects with emphasis on problems on both sides: on the side of beneficiaries and on the side of the Agricultural Paying Agency. The goal of the paper is to show problems which occurred in all phases of realization of projects under the measure 3.1 with the aim to avoid the appearance of the same problems in following programming periods.

  16. The KISS Project - Exploring the magmatic system beneath Kamchatka's volcanoes

    Science.gov (United States)

    Luehr, Birger-G.; Shapiro, Nikolai; Abkadyrov, Ilyas; Sens-Schönfelder, Christoph; Koulakov, Ivan; Jakovlev, Andrey; Abramenkov, Sergey; Saltykov, Vadim A.; Heit, Benjamin; Weber, Michael; Gordeev, Evgeny I.; Chebrov, Victor N.

    2016-04-01

    In a joint initiative of GFZ with Russian (IPGG, IVS, KGBS) and a French partner (IPGP) a temporary seismological network has been installed around the Klyuchevskoy volcanic group in Central Kamchatka. The Klyuchevskoy volcanic group is an ensemble of 13 stratovolcanoes with very different compositions and eruption styles in a ~70km diameter area which produced at least 30 VEI≥2 episodes during the last 15 years. Latest activity of the highest volcano Klyuchevskoy (4754 m) was in spring 2015. The group is located right on the triple junction between Asian, Pacifc and North American plates where the Hawaiian-Emperor seamount chain separates the Aleutian and the Kuril-Kamchatka trenches. The complex setting presumably leads to processes like increased melting at slab edges and/or accelerated mantle flow which affect the volcanism and might be responsible for the unparalleled concentration of volcanic activity in the Klyuchevskoy group. Due to the difficult field conditions and special permitting regulations seismological investigations have been rare in Kamchatka. In this consortium we build strongly on the experience of the Kamchatkan partners for permitting and logistics. Installation was done to about 50% by helicopter. Funding was provided via a grant from the Russian Science Foundation (grant 14-47-00002) to the IVS/KBGS/IPGG, the GFZ, and the IPGP. 60 of the stations were provided by the GFZ instrument pool GIPP. Including the permanent stations operated by KGBS and temporary stations provided by the partners, the network consist of 98 stations and will record earthquakes volcanic signals and the ambient field over one year in an area of approximately 150 by 150km.

  17. Characterization of a [open quotes]kissing[close quotes] hairpin complex derived from the human immunodeficiency virus genome

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Kung-Yao; Tinoco, Ignacio Jr. (Lawrence Berkeley Lab., CA (United States))

    1994-08-30

    Base-pair formation between hairpin loops-a [open quotes]kissing[close quotes] complex-is an RNA-folding motif that links two elements of RNA secondary structure. It is also a unique protein recognition site involved in regulation of ColE1 plasmid DNA replication. The trans-activation response element (TAR), a hairpin and bulge at the 5[prime] end of the untranslated leader region of the human immunodeficiency virus 1 mRNA, enhances the transcription of the virus and is necessary for viral replication. Gel electrophoresis and absorbance melting curves indicate that a synthesized RNA hairpin (Tar*-16) with a loop sequence complementary to the TAR loop sequence (CUGGGA) associates specifically with a 16-nucleotide TAR hairpin (Tar-16) to form a stable complex. RNase T1 probing indicates that the three guanines in the Tar-16 loop become inaccessible in the complex. NMR imino proton spectra reveal that 5 base pairs are formed between the two hairpin loops (Tar-16 and Tar*-16); only the adenine at the 3[prime] terminus of the TAR loop does not form a base pair with the 5[prime]-terminal uracil of the complementary loop. A 14-nucleotide hairpin [CCUA-(UCCCAG)UAGG] with a loop sequence complementary to the TAR loop is conserved within the gag gene of human immunodeficiency virus 1. A synthesized RNA hairpin corresponding to this conserved sequence also binds to the Tar-16 hairpin with high affinity. It is possible that the same RNA loop-loop interaction occurs during the viral life cycle. 40 refs., 7 figs.

  18. Efficacy of Tailored Physical Activity or Chronic Pain Self-Management Programme on return to work for sick-listed citizens

    DEFF Research Database (Denmark)

    Andersen, Lotte Nygaard; Juul-Kristensen, Birgit; Sørensen, Thomas Lund

    2015-01-01

    . In contrast, no benefit of TPA and CPSMP was evident regarding work ability, kinesiophobia or physical capacity after 3 months of follow-up. CONCLUSIONS: The results suggest that TPA is a promising intervention to facilitate return to work and reduce pain among sick-listed citizens with pain related......OBJECTIVES: The aim was to evaluate the efficacy of 'Tailored Physical Activity' (TPA) and a 'Chronic Pain Self-management Programme' (CPSMP) compared with a reference group (REF) on return to work after 3 months as sick-listed citizens with pain related to the back or the upper body. METHODS......: Using a randomised controlled trial design all participants (n= 141) received health guidance for 1.5 hours and were randomised to TPA, CPSMP or REF. Characteristics of participants were collected from a questionnaire. The primary endpoint was proportion of participants returned to work as registered...

  19. A comparison between aortobifemoral bypass and aortoiliac kissing stents in patients with complex aortoiliac obstructive disease.

    Science.gov (United States)

    Dorigo, Walter; Piffaretti, Gabriele; Benedetto, Filippo; Tarallo, Antonino; Castelli, Patrizio; Spinelli, Francesco; Fargion, Aaron; Pratesi, Carlo

    2017-01-01

    The aim of this study was to retrospectively compare early and late results of aortobifemoral bypass and endovascular recanalization with the kissing stent technique in the management of TransAtlantic Inter-Society Consensus II (TASC II) C and D lesions in the aortoiliac district in a multicenter study. From January 2006 to December 2013, 210 open and endovascular interventions for TASC II class C and D aortoiliac obstructive lesions were performed at three Italian teaching hospitals. In all the cases, the intervention was performed for aortic and bilateral iliac involvement. An aortobifemoral bypass was performed in 82 patients (group 1); in the remaining 128, an endovascular recanalization with the kissing stent technique was carried out (group 2). Early results in the two groups were compared with the χ(2) test. Follow-up results were analyzed with Kaplan-Meier curves and compared with log-rank test. Univariate and multivariate (forward Cox regression) analysis to identify potentially significant predictors of primary patency in the whole study group was performed. Patients in group 2 were more frequently female and more frequently had diabetes and arterial hypertension than patients in group 1. The indication for surgical intervention was the presence of critical limb ischemia in 29 cases in group 1 (35%) and in 31 cases in group 2 (24%; P = .07). Technical success in group 2 was 98.5%; two patients required immediate conversion to open surgery for iliac rupture. There was one perioperative death in group 1 (mortality rate, 1%; P = .2 in comparison with group 2). Four perioperative thromboses occurred, two in group 1 and two in group 2 (in one case requiring conversion to open surgical intervention), and no amputations at 30 days were recorded. Cumulative postoperative local and systemic complications occurred in 17 patients in group 1 (20.5%) and in 9 patients in group 2 (7%; P kissing stent technique provided similar satisfactory early and late results

  20. A survey of radiation treatment planning peer-review activities in a provincial radiation oncology programme: current practice and future directions

    Science.gov (United States)

    Brundage, Michael; Foxcroft, Sophie; McGowan, Tom; Gutierrez, Eric; Sharpe, Michael; Warde, Padraig

    2013-01-01

    Objectives To describe current patterns of practice of radiation oncology peer review within a provincial cancer system, identifying barriers and facilitators to its use with the ultimate aim of process improvement. Design A survey of radiation oncology programmes at provincial cancer centres. Setting All cancer centres within the province of Ontario, Canada (n=14). These are community-based outpatient facilities overseen by Cancer Care Ontario, the provincial cancer agency. Participants A delegate from each radiation oncology programme filled out a single survey based on input from their multidisciplinary team. Outcome measures Rated importance of peer review; current utilisation; format of the peer-review process; organisation and timing; case attributes; outcomes of the peer-review process and perceived barriers and facilitators to expanding peer-review processes. Results 14 (100%) centres responded. All rated the importance of peer review as at least 8/10 (10=extremely important). Detection of medical error and improvement of planning processes were the highest rated perceived benefits of peer review (each median 9/10). Six centres (43%) reviewed at least 50% of curative cases; four of these centres (29%) conducted peer review in more than 80% of cases treated with curative intent. Fewer than 20% of cases treated with palliative intent were reviewed in most centres. Five centres (36%) reported usually conducting peer review prior to the initiation of treatment. Five centres (36%) recorded the outcomes of peer review on the medical record. Thirteen centres (93%) planned to expand peer-review activities; a critical mass of radiation oncologists was the most important limiting factor (median 6/10). Conclusions Radiation oncology peer-review practices can vary even within a cancer system with provincial oversight. The application of guidelines and standards for peer-review processes, and monitoring of implementation and outcomes, will require effective knowledge

  1. The effectiveness of a physical activity stimulation programme for children with cerebral palsy on social participation, self-perception and quality of life: a randomized controlled trial.

    Science.gov (United States)

    Van Wely, Leontien; Balemans, Astrid Cj; Becher, Jules G; Dallmeijer, Annet J

    2014-10-01

    To determine the effects of a six-month physical activity stimulation programme on social participation, self-perception and quality of life in children with cerebral palsy. Multicentre randomized controlled trial with concealed allocation, blinded assessments and intention-to-treat analysis. Paediatric physiotherapy practices, special schools for children with a disability, and the child's own home. Forty-nine children with spastic cerebral palsy (28 male), aged 7-13 years, able to walk with and without walking aids. The intervention group followed a six-month physical activity stimulation programme involving counselling through motivational interviewing, home-based physiotherapy and four months of fitness training. The control group continued regular paediatric physiotherapy. Outcomes included social participation in domestic life, social participation in recreation and leisure (Life-Habits for Children questionnaire and Children's Assessment of Participation and Enjoyment questionnaire), self-perception (Harter's Self-Perception Profile for Children) and parent-reported quality of life (Cerebral Palsy Quality of Life Questionnaire). Assessments were performed at baseline, at six months (except quality of life) and at twelve months. Intervention resulted in a positive effect on social participation in domestic life at twelve months (mean between-group difference = 0.9, 95% confidence interval (CI) = 0.1 to 1.7 [1-10 scale], P = 0.03), but not at six months. No significant effects were found for social participation in recreation and leisure, self-perception at six months and twelve months or for quality of life at twelve months. The combination of counselling, home-based physiotherapy and fitness training was not effective in improving social participation in recreation and leisure, self-perception or quality of life, but did show a potential for improving social participation in domestic life over the longer term. © The Author(s) 2013.

  2. Olympic Partner Programme

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    @@ The Olympic Partner Programme (TOP) is an international Olympic marketing programme created by the International Olympic Committee (IOC),which includes the Organising Committees of the Games,the National Olympic Committees and the TOP Partners.

  3. GLP-1R Signaling Directly Activates Arcuate Nucleus Kisspeptin Action in Brain Slices but Does not Rescue Luteinizing Hormone Inhibition in Ovariectomized Mice During Negative Energy Balance

    Science.gov (United States)

    Heppner, Kristy M.; Baquero, Arian F.; True, Cadence; Grove, Kevin L.

    2017-01-01

    Abstract Kisspeptin (Kiss1) neurons in the hypothalamic arcuate nucleus (ARC) are key components of the hypothalamic-pituitary-gonadal axis, as they regulate the basal pulsatile release of gonadotropin releasing hormone (GnRH). ARC Kiss1 action is dependent on energy status, and unmasking metabolic factors responsible for modulating ARC Kiss1 neurons is of great importance. One possible factor is glucagon-like peptide 1 (GLP-1), an anorexigenic neuropeptide produced by brainstem preproglucagon neurons. Because GLP fiber projections and the GLP-1 receptor (GLP-1R) are abundant in the ARC, we hypothesized that GLP-1R signaling could modulate ARC Kiss1 action. Using ovariectomized mice, we found that GLP-producing fibers come in close apposition with ARC Kiss1 neurons; these neurons also contain Glp1r mRNA. Electrophysiological recordings revealed that liraglutide (a long-acting GLP-1R agonist) increased action potential firing and caused a direct membrane depolarization of ARC Kiss1 cells in brain slices. We determined that brainstem preproglucagon mRNA is decreased after a 48-h fast in mice, a negative energy state in which ARC Kiss1 expression and downstream GnRH/luteinizing hormone (LH) release are potently suppressed. However, activation of GLP-1R signaling in fasted mice with liraglutide was not sufficient to prevent LH inhibition. Furthermore, chronic central infusions of the GLP-1R antagonist, exendin(9–39), in ad libitum–fed mice did not alter ARC Kiss1 mRNA or plasma LH. As a whole, these data identify a novel interaction of the GLP-1 system with ARC Kiss1 neurons but indicate that CNS GLP-1R signaling alone is not critical for the maintenance of LH during fasting or normal feeding. PMID:28144621

  4. GLP-1R Signaling Directly Activates Arcuate Nucleus Kisspeptin Action in Brain Slices but Does not Rescue Luteinizing Hormone Inhibition in Ovariectomized Mice During Negative Energy Balance.

    Science.gov (United States)

    Heppner, Kristy M; Baquero, Arian F; Bennett, Camdin M; Lindsley, Sarah R; Kirigiti, Melissa A; Bennett, Baylin; Bosch, Martha A; Mercer, Aaron J; Rønnekleiv, Oline K; True, Cadence; Grove, Kevin L; Smith, M Susan

    2017-01-01

    Kisspeptin (Kiss1) neurons in the hypothalamic arcuate nucleus (ARC) are key components of the hypothalamic-pituitary-gonadal axis, as they regulate the basal pulsatile release of gonadotropin releasing hormone (GnRH). ARC Kiss1 action is dependent on energy status, and unmasking metabolic factors responsible for modulating ARC Kiss1 neurons is of great importance. One possible factor is glucagon-like peptide 1 (GLP-1), an anorexigenic neuropeptide produced by brainstem preproglucagon neurons. Because GLP fiber projections and the GLP-1 receptor (GLP-1R) are abundant in the ARC, we hypothesized that GLP-1R signaling could modulate ARC Kiss1 action. Using ovariectomized mice, we found that GLP-producing fibers come in close apposition with ARC Kiss1 neurons; these neurons also contain Glp1r mRNA. Electrophysiological recordings revealed that liraglutide (a long-acting GLP-1R agonist) increased action potential firing and caused a direct membrane depolarization of ARC Kiss1 cells in brain slices. We determined that brainstem preproglucagon mRNA is decreased after a 48-h fast in mice, a negative energy state in which ARC Kiss1 expression and downstream GnRH/luteinizing hormone (LH) release are potently suppressed. However, activation of GLP-1R signaling in fasted mice with liraglutide was not sufficient to prevent LH inhibition. Furthermore, chronic central infusions of the GLP-1R antagonist, exendin(9-39), in ad libitum-fed mice did not alter ARC Kiss1 mRNA or plasma LH. As a whole, these data identify a novel interaction of the GLP-1 system with ARC Kiss1 neurons but indicate that CNS GLP-1R signaling alone is not critical for the maintenance of LH during fasting or normal feeding.

  5. Optical coherence tomography assessment of a complex bifurcation lesion treated with double kissing Crush technique

    Science.gov (United States)

    Cai, Jin-Zan; Zhang, Yao-Jun; Xu, Tian; Zhu, Yong-Xiang; Mao, Chen-Yu; Bourantas, Christos V.; Crake, Tom; Chen, Shao-Liang

    2017-01-01

    Abstract The DEFINITION (Impact of the complexity of bifurcation lesions treated with drug-eluting stents) study has provided a novel classification to evaluate the complexity of coronary bifurcation lesion according to coronary angiography, but angiographic imaging due to its low resolution and inherited limitation may result in an inaccurate adjudication. We used optical coherence tomography (OCT) to further evaluate the coronary characteristics in a patient with “simple” bifurcation lesion which was classified by the DEFINITION criteria. However, a “complex” bifurcation lesion was defined and confirmed according to the OCT results. A double kissing Crush stenting approach was adopted to treat this “complex” case finally. The immediate and long-term angiographic and OCT results were excellent. OCT may be useful imaging modality to classify complexity of coronary bifurcation lesion and subsequently guide its treatment strategy. PMID:28072714

  6. Perspectives on kiss-and-run: role in exocytosis, endocytosis, and neurotransmission.

    Science.gov (United States)

    Alabi, AbdulRasheed A; Tsien, Richard W

    2013-01-01

    Regulated exocytosis and endocytosis are critical to the function of many intercellular networks, particularly the complex neural circuits underlying mammalian behavior. Kiss-and-run (KR) is an unconventional fusion between secretory vesicles and a target membrane that releases intravesicular content through a transient, nanometer-sized fusion pore. The fusing vesicle retains its gross shape, precluding full integration into the planar membrane, and enough molecular components for rapid retrieval, reacidification, and reuse. KR makes judicious use of finite presynaptic resources, and mounting evidence suggests that it influences synaptic information transfer. Here we detail emerging perspectives on KR and its role in neurotransmission. We additionally formulate a restraining force hypothesis as a plausible mechanistic basis for KR and its physiological modulation in small nerve terminals. Clarification of the mechanism and function of KR has bearing on understanding the kinetic transitions underlying SNARE-mediated fusion, interactions between vesicles and their local environment, and the influence of release dynamics on neural information processing.

  7. RNA LEGO: magnesium-dependent formation of specific RNA assemblies through kissing interactions.

    Science.gov (United States)

    Horiya, Satoru; Li, Xianglan; Kawai, Gota; Saito, Ryota; Katoh, Akira; Kobayashi, Koh; Harada, Kazuo

    2003-07-01

    The high affinity and specificity of nucleic acid base complementarity has been proven to be a powerful method for constructing specific molecular assemblies. On the other hand, recent structural studies of RNA have revealed the wide range of tertiary interactions utilized in RNA folding, which may potentially be used as tools for the design of specific macromolecular assemblies. Here, RNA building blocks containing two hairpin loops, based on the dimerization initiation site (DIS) of HIV RNA, connected by a short linker were used to construct large RNA assemblies through hairpin loop-loop ("kissing") interactions. We show that specific linear and circular assemblies can be constructed in a magnesium-dependent manner using several non-self-complementary loop-loop interactions designed in this study. These results show that the use of RNA tertiary interactions may broaden the repertoire of nucleic acid-based nanostructures.

  8. Gerstmann meets Geschwind: a crossing (or kissing) variant of a subcortical disconnection syndrome?

    Science.gov (United States)

    Kleinschmidt, Andreas; Rusconi, Elena

    2011-12-01

    That disconnection causes clinical symptoms is a very influential concept in behavioral neurology. Criteria for subcortical disconnection usually are symptoms that are distinct from those following cortical lesions and damage to a single, long-range fiber tract. Yet, a recent study combining functional magnetic resonance imaging and fiber tracking concluded that a focal lesion in left parietal white matter provides the only tenable explanation for pure Gerstmann's syndrome, an enigmatic tetrad of acalculia, agraphia, finger agnosia, and left-right disorientation. Such a lesion would affect not only a single fiber tract but crossing or "kissing" of different fiber tracts and hence disconnect separate cortical networks. As fiber crossing is prominent in the cerebral white matter, the authors propose an extension to the subcortical disconnection framework that opens the door to ascribing a more diversified clinical phenomenology to white matter damage and ensuing disconnection than has been the case so far.

  9. Radiologic findings consistent with kissing spines syndrome in Chilean thoroughbreds horses

    Directory of Open Access Journals (Sweden)

    Dario Infante

    2016-12-01

    Full Text Available Alterations in the thoracolumbar spine of racehorses are frequent and often significantly decrease their athletic performance. The most common thoracolumbar alteration in thoroughbred horses is kissing spines syndrome (KSS. The narrowing of the interespinous space, generally located between T14-T15 and T15-T16, produces this syndrome. A radiographic study was performed to 30 thoroughbred horses on the segment between T12 and T18. Two latero-lateral views from digital equipment were obtained of the T12 to T18 segment of each horse, the images were analyze and the radiographic findings established the KSS according to a grading scale. The study sample was homogeneous and the results were similar to other radiographic findings of KSS occurring in segments T14-T15 and T15-16.

  10. Hierarchy of kissing numbers for exceptional Lie symmetry groups in high energy physics

    Energy Technology Data Exchange (ETDEWEB)

    El Naschie, M.S. [Donghua University, Shanghai (China); Department of Physics University of Alexandria, Alexandria (Egypt)], E-mail: Chaossf@aol.com

    2008-01-15

    We are constructing a hierarchy of kissing numbers representing singular contact points of hyper-spheres in exceptional Lie symmetry groups lattice arrangement embedded in the 26 dimensional bosonic strings spacetime. That way we find a total number of points and dimensions equal to 548. This is 52 more than the order of E{sub 8}E{sub 8} of heterotic string theory and leads to the prediction of 69 elementary particles at an energy scale under 1 T. In other words, our mathematical model predicts nine more particles than what is currently experimentally known to exist in the standard model of high energy physics namely only 60. The result is thus in full agreement with all our previous theoretical findings.

  11. Self-reported care activities in a home-based intervention programme for families with multiple problems

    NARCIS (Netherlands)

    Tausendfreund, Tim; Metselaar, Janneke; Conradie, Jelte; de Groot, Maria Helena; Schipaanboord, Nicolien; Knot-Dickscheit, Jana; Grietens, Hans; Knorth, Erik J.

    2015-01-01

    The purpose of this paper is to describe the development and application of the KIPP-list of care activities. The acronym KIPP stands for Knowledge and Insight into Primary Processes. The instrument is intended as a tool for family coaches to systematically report care activities conducted in the Du

  12. Evaluation of a school-based intervention programme to promote physical activity: an application of the theory of planned behavior.

    Science.gov (United States)

    Tsorbatzoudis, Haralambos

    2005-12-01

    The Theory of Planned Behavior provides a useful framework to study attitudes toward participation in physical activity. The objective of the study was to test the effectiveness of an intervention in manipulating the variables of the Theory of Planned Behavior and exercise habits with 366 high school students (M = 14.2 yr., SD = .7; 201 boys and 165 girls). The students were divided into intervention and control groups. A questionnaire to measure components of the theory, and the Baecke Questionnaire of Habitual Activity measuring exercise habits, were administered. The intervention lasted 12 wk. and included posters and lectures promoting participation in physical activity. Analyses showed the intervention was effective in improving attitudes towards physical activity, perceived behavioral control, intention, and self-reported actual behavior, but it was ineffective for improving attitude strength, subjective norms, and role identity. The results provide useful information for physical education teachers interested in promoting students' positive attitudes towards physical activity.

  13. The impact of a bodyweight and physical activity intervention (BeWEL) initiated through a national colorectal cancer screening programme: randomised controlled trial.

    Science.gov (United States)

    Anderson, Annie S; Craigie, Angela M; Caswell, Stephen; Treweek, Shaun; Stead, Martine; Macleod, Maureen; Daly, Fergus; Belch, Jill; Rodger, Jackie; Kirk, Alison; Ludbrook, Anne; Rauchhaus, Petra; Norwood, Patricia; Thompson, Joyce; Wardle, Jane; Steele, Robert J C

    2014-03-07

    To evaluate the impact of a diet and physical activity intervention (BeWEL) on weight change in people with a body mass index >25 weight (kg)/height (m)(2) at increased risk of colorectal cancer and other obesity related comorbidities. Multicentre, parallel group, randomised controlled trial. Four Scottish National Health Service health boards. 329 overweight or obese adults (aged 50 to 74 years) who had undergone colonoscopy after a positive faecal occult blood test result, as part of the national bowel screening programme, and had a diagnosis of adenoma confirmed by histopathology. 163 were randomised to intervention and 166 to control. Participants were randomised to a control group (weight loss booklet only) or 12 month intervention group (three face to face visits with a lifestyle counsellor plus monthly 15 minute telephone calls). A goal of 7% reduction in body weight was set and participants received a personalised energy prescription (2508 kJ (600 kcal) below that required for weight maintenance) and bodyweight scales. Motivational interviewing techniques explored self assessed confidence, ambivalence, and personal values concerning weight. Behavioural strategies included goal setting, identifying intentions of implementation, self monitoring of body weight, and counsellor feedback about reported diet, physical activity, and weight change. The primary outcome was weight change over 12 months. Secondary outcomes included changes in waist circumference, blood pressure, fasting cardiovascular biomarkers, and glucose metabolism variables, physical activity, diet, and alcohol consumption. At 12 months, data on the primary outcome were available for 148 (91%) participants in the intervention group and 157 (95%) in the control group. Mean weight loss was 3.50 kg (SD 4.91) (95% confidence interval 2.70 to 4.30) in the intervention group compared with 0.78 kg (SD 3.77) (0.19 to 1.38) in the control group. The group difference was 2.69 kg (95% confidence interval 1

  14. UNESCO's Ethics Education Programme.

    NARCIS (Netherlands)

    Have, H.A.M.J. ten

    2008-01-01

    Unesco initiated the Ethics Education Programme in 2004 at the request of member states to reinforce and increase the capacities in the area of ethics teaching. The programme is focused on providing detailed information about existing teaching programmes. It also develops and promotes teaching throu

  15. A reappraisal of the virtues of private sector employment programmes

    DEFF Research Database (Denmark)

    Graversen, Brian Krogh; Jensen, Peter

    2010-01-01

    This paper evaluates the employment effects of active labour market programmes for Danish welfare benefit recipients, focusing on private sector employment (PSE) programmes. Using a latent variable model that allows for heterogeneous treatment effects among observationally identical persons, we...

  16. Investigations on tunneling and kissing bond defects in FSW joints for dissimilar aluminum alloys

    Energy Technology Data Exchange (ETDEWEB)

    Khan, Noor Zaman, E-mail: noor_0315@yahoo.com [Department of Mechanical Engineering, Jamia Millia Islamia (A Central University), New Delhi (India); Siddiquee, Arshad Noor; Khan, Zahid A. [Department of Mechanical Engineering, Jamia Millia Islamia (A Central University), New Delhi (India); Shihab, Suha K. [Department of Mechanical Engineering, Engineering College, Diyala University, Diyala (Iraq)

    2015-11-05

    In this paper an attempt has been made to investigate the effect of two Friction Stir Welding (FSW) parameters i.e. tool pin offset and tool plunge depth on the formation of defects such as tunnel (tunneling defect) and kissing bond (KB) during welding of dissimilar aluminum alloys. 4.75 mm thick plates of AA5083-H116 and AA6063-T6 were welded using a novel work-fixture developed in-house which, apart from clamping the plated also imparted continuous variation of offset on both side of the faying line. The tunneling defect was modeled as a function of offset and plunge depth. The welds were characterised using optical microscopy (OM), scanning electron microscopy (SEM) and mechanical testing. The causes of such defects have been analyzed and discussed and recommendations have been made to prevent their occurrence. The findings of the study have revealed that the tunneling defects are formed at all offset (including zero offset) values towards stronger material (advancing side). And the cross-section of the tunnel varied with the amount of offset. Further, KBs are formed at the interface for all pin offset values except 0.5 mm towards weaker material and high plunge depth resulting in the poor mechanical properties. - Highlights: • Two dissimilar aluminum alloys are welded using FSW. • Formation of kissing bond and tunneling defects are investigated. • Defects are formed at pin offsets towards stronger material and also without offset. • The size of tunnel reduces significantly by increasing the plunge depth. • Tool pin offset towards weaker material prevent tunneling defects.

  17. The duplicated longitudinal epiphysis or ''kissing delta phalanx'': evolution and variation in three different disorders

    Energy Technology Data Exchange (ETDEWEB)

    Elliott, Alison M. [Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E 0W3, Winnipeg, Manitoba (Canada); Evans, Jane A.; Chudley, Albert E. [Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E 0W3, Winnipeg, Manitoba (Canada); Department of Pediatrics and Child Health, University of Manitoba and Health Sciences Centre, Winnipeg, Manitoba (Canada); Reed, Martin H. [Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E 0W3, Winnipeg, Manitoba (Canada); Department of Pediatrics and Child Health, University of Manitoba and Health Sciences Centre, Winnipeg, Manitoba (Canada); Department of Radiology, University of Manitoba and Health Sciences Centre, Winnipeg, Manitoba (Canada)

    2004-06-01

    The delta phalanx, also known as the delta bone, or longitudinal epiphyseal bracket (LEB), has been described in a variety of syndromes and dysplasias. However, the ''kissing delta phalanx'' is not as well recognized in the literature; it consists of a duplicated longitudinal bracketed epiphysis, or a complex of two adjacent delta bones, with opposing convex portions facing each other. Magnetic resonance imaging of the kissing delta phalanx has not been previously described. To describe the evolution, variation and associated osseous anomalies of the kissing delta phalanx in three patients with distinct distal limb malformations using both plain films and magnetic resonance imaging. Patient 1 had Rubinstein-Taybi syndrome and, in addition to a kissing delta phalanx in both feet, had corresponding delta metatarsals (MT1). Patient 2 had Cenani-Lenz syndactyly with distinct variation of the kissing delta phalanx in each hand. He had a disorganized appearance to the phalanges, metacarpal fusions and carpal coalitions. Patient 3 had an isolated oligosyndactyly of the left hand with metacarpal fusions and carpal coalitions. All three patients were followed over time. We describe two types of kissing delta phalanges: separated (without fusion of the corresponding epiphyseal brackets) and nonseparated (with fusion of the corresponding epiphyseal brackets). Both types can be seen in the same patient and are a reflection of the relative degree of segmentation of the two delta bones. (orig.)

  18. Why some do but most don't. Barriers and enablers to engaging low-income groups in physical activity programmes: a mixed methods study

    Directory of Open Access Journals (Sweden)

    Fox Kenneth R

    2011-06-01

    Full Text Available Abstract Background The beneficial effect of physical activity for the prevention of a range of chronic diseases is widely acknowledged. These chronic conditions are most pronounced in economically disadvantaged groups where physical activity levels are consistently lower, yet this group is particularly difficult to recruit and retain in physical activity programmes. This study examined the perceptions of participants, non-participants, and exercise leaders in a low-income area regarding barriers, motives, and enabling factors for organised physical activity with a view to improving recruitment and retention. Methods A mixed methods research approach was adopted to guide data collection and analysis. A survey, incorporating the Motivation for Physical Activity Measure - Revised (MPAM-R, was used to assess the motivations of 152 physical activity session participants in a highly deprived suburban neighbourhood. The MPAM-R data were analysed using t tests, analyses of variance to estimate age, body mass index, and activity mode differences and Pearson's correlation coefficient to address associations. Semi-structured interviews were also conducted with 33 local residents who did not participate in activity sessions and with 14 activity session leaders. All interviews were audio-taped, transcribed verbatim and analyzed using an inductive thematic approach. Results Participants reported cost, childcare, lack of time and low awareness as barriers to joining activity classes. The need for support, confidence and competence in order to take up activity was widely expressed, particularly among women. Once people are active, high levels of social interaction, interest and enjoyment are associated with improved levels of retention, with different types of physical activity scoring differently on these factors. Conclusions This study suggests that some factors such as cost, the fear of 'walking in alone', accessibility of facilities, and appropriate

  19. Expert validation of fit-for-purpose guidelines for designing programmes of assessment.

    NARCIS (Netherlands)

    Dijkstra, J.; Galbraith, R.; Hodges, B.D.; McAvoy, P.A.; McCrorie, P.; Southgate, L.J.; Vleuten, C.P.M. van der; Wass, V.; Schuwirth, L.W.

    2012-01-01

    ABSTRACT: BACKGROUND: An assessment programme, a purposeful mix of assessment activities, is necessary to achieve a complete picture of assessee competence. High quality assessment programmes exist, however, design requirements for such programmes are still unclear. We developed guidelines for desig

  20. New and recently finalised activities within the NKS Programmes for Nordic cooperation on nuclear reactor safety and emergency preparedness

    DEFF Research Database (Denmark)

    Andgren, Karin; Andersson, Kasper Grann; Magnússon, Sigurður M.

    2015-01-01

    Over the years, NKS has provided funding for hundreds of research activities in fields comprising reactor safety, decommissioning, nuclear and radiological emergency preparedness, and management of radioactive waste. Advanced technologies and methods developed under the NKS framework have been used...

  1. Kissing Cousins

    Science.gov (United States)

    Wyatt, Neal

    2010-01-01

    In the library world, there seems to be a fundamental conflict between reference and readers' advisory (RA). But in fact, the two are similar in approach, an insight that goes back to James Wyer's 1930 "Reference Work: A Textbook for Students of Library Work and Librarians." It defined the reference librarian as a mediator of information,…

  2. Kissing Cousins

    Science.gov (United States)

    Wyatt, Neal

    2010-01-01

    In the library world, there seems to be a fundamental conflict between reference and readers' advisory (RA). But in fact, the two are similar in approach, an insight that goes back to James Wyer's 1930 "Reference Work: A Textbook for Students of Library Work and Librarians." It defined the reference librarian as a mediator of information,…

  3. A Statistical Programme Assignment Model

    DEFF Research Database (Denmark)

    Rosholm, Michael; Staghøj, Jonas; Svarer, Michael

    When treatment effects of active labour market programmes are heterogeneous in an observable way  across the population, the allocation of the unemployed into different programmes becomes a particularly  important issue. In this paper, we present a statistical model designed to improve the present...... assignment  mechanism, which is based on the discretionary choice of case workers. This is done in a duration model context, using the timing-of-events framework to identify causal effects. We compare different assignment  mechanisms, and the results suggest that a significant reduction in the average...... duration of unemployment spells may result if a statistical programme assignment model is introduced. We discuss several issues regarding the  plementation of such a system, especially the interplay between the statistical model and  case workers....

  4. A Kiss Lajos-díj 2016. évi nyertese: dr. Póczos Rita [Winner of the 2016 Lajos Kiss Prize: Dr Rita Póczos

    Directory of Open Access Journals (Sweden)

    Hoffmann, István

    2016-12-01

    Full Text Available The Lajos Kiss Prize, awarded every two or three years since 2006, is considered to be a highly prestigious award for young onomasticians. The 2016 winner of the prize is Dr Rita Póczos, senior lecturer at the Department of Hungarian Linguistics of the University of Debrecen. She was given this award for her contribution to Hungarian Historical Onomastics, especially for her research on place names.

  5. A Kiss Lajos-díj 2014. évi nyertese: dr. Slíz Mariann [The 2014 winner of Lajos Kiss Prize: Dr Mariann Slíz

    Directory of Open Access Journals (Sweden)

    Hoffmann, István

    2014-12-01

    Full Text Available The Lajos Kiss Prize, awarded every two or three years since 2006, is considered to be a highly prestigious award for young onomasticians. The 2014 winner of the prize is Dr Mariann Slíz, senior lecturer at the Department of Hungarian Linguistics and Finno-Ugric Studies of Eötvös Loránd University, Budapest. She earned this award for her contribution to Hungarian Onomastics, especially in research on personal names.

  6. Impact of a school-based intervention on nutritional education and physical activity in primary public schools in Chile (KIND) programme study protocol: cluster randomised controlled trial.

    Science.gov (United States)

    Bustos, Nelly; Olivares, Sonia; Leyton, Bárbara; Cano, Marcelo; Albala, Cecilia

    2016-12-03

    Chile has suffered a fast increase in childhood obesity in the last 10 years. As a result, several school programmes have been implemented, however the effectiveness of these needs to be evaluated to identify and prioritize strategies to curve this trend. Cluster randomized controlled trial. Twelve primary public schools chosen at random over three regions of the country will take part in this study. The sample size consisted of a total of 1,655 children. For each region one school will be selected for each of the three nutritional intervention modes and one school will be selected as the control group. The intervention modes consist of the following: Healthy Kiosk and nutritional education (KSEAN); Optimized physical activity (AFSO); Healthy Kiosk and nutritional education (KSEAN) + optimized physical activity (AFSO); Control group. The effectiveness of each intervention will be evaluated by determining the nutritional condition of each child by measuring percentage of body fat, BMI and the z-score of the BMI. This study will also identify the eating behaviours, nutritional knowledge and fitness of each child, along with the effective time of moderate activity during physical education classes. A protocol to evaluate the effectiveness of a school based intervention to control and/or reduce the rates of childhood obesity for children between 6 and 10 years of age was developed. The protocol was developed in line with the Declaration of Helsinski, the Nüremberg Code and the University of Chile Guidelines for ethical committees, and was approved by the INTA, Universidad de Chile ethical committee on Wednesday 12 March 2014. There is consensus among researchers and health and education personnel that schools are a favourable environment for actions to prevent and/or control childhood obesity. However a lack of evidence on the effectiveness of interventions to date has led some to question the wisdom of allocating resources to programmes. This is the first study

  7. Scaling up strategies of the chronic respiratory disease programme of the European Innovation Partnership on Active and Healthy Ageing

    DEFF Research Database (Denmark)

    Bousquet, J; Farrell, J; Crooks, G;

    2016-01-01

    Action Plan B3 of the European Innovation Partnership on Active and Healthy Ageing (EIP on AHA) focuses on the integrated care of chronic diseases. Area 5 (Care Pathways) was initiated using chronic respiratory diseases as a model. The chronic respiratory disease action plan includes (1) AIRWAYS ...

  8. Implementation of a metrology programme to provide traceability for radionuclides activity measurements in the CNEN Radiopharmaceuticals Producers Centers

    Energy Technology Data Exchange (ETDEWEB)

    Andrade, Erica A.L. de; Braghirolli, Ana M.S.; Tauhata, Luiz; Gomes, Regio S.; Silva, Carlos J., E-mail: erica@ien.gov.br [Instituto de Engenharia Nuclear (IEN/CNEN-RJ), Rio de Janeiro, RJ (Brazil); Delgado, Jose U.; Oliveira, Antonio E.; Iwahara, Akira, E-mail: ealima@ird.gov.br [Instituto de Radioprotecao e Dosimetria (IRD/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2013-07-01

    The commercialization and use of radiopharmaceuticals in Brazil are regulated by Agencia Nacional de Vigilancia Sanitaria (ANVISA) which require Good Manufacturing Practices (GMP) certification for Radiopharmaceuticals Producer Centers. Quality Assurance Program should implement the GMP standards to ensure radiopharmaceuticals have requirements quality to proving its efficiency. Several aspects should be controlled within the Quality Assurance Programs, and one of them is the traceability of the Radionuclides Activity Measurement in radiopharmaceuticals doses. The quality assurance of activity measurements is fundamental to maintain both the efficiency of the nuclear medicine procedures and patient and exposed occupationally individuals safety. The radiation doses received by patients, during the nuclear medicine procedures, is estimated according to administered radiopharmaceuticals quantity. Therefore it is very important either the activity measurements performed in radiopharmaceuticals producer centers (RPC) as the measurements performed in nuclear medicine services are traceable to national standards. This paper aims to present an implementation program to provide traceability to radionuclides activity measurements performed in the dose calibrators(well type ionization chambers) used in Radiopharmaceuticals Producer Center placed in different states in Brazil. The proposed program is based on the principles of GM Pand ISO 17025 standards. According to dose calibrator performance, the RPC will be able to provide consistent, safe and effective radioactivity measurement to the nuclear medicine services. (author)

  9. A label-free kissing complexes-induced fluorescence aptasensor using DNA-templated silver nanoclusters as a signal transducer.

    Science.gov (United States)

    Zhang, Kai; Wang, Ke; Zhu, Xue; Xie, Minhao

    2016-04-15

    Riboswitches are complex folded RNA domains that serve as receptors for specific metabolites which identified in prokaryotes. They are comprised of a biosensor that includes the binding site for a small ligand and they respond to association with this ligand by undergoing a conformational change. In the present study, we report on the integration of silver nanoclusters (AgNCs) and riboswitches for the development of a kissing complexes-induced aptasensor (KCIA). We specifically apply the tunable riboswitches properties of this strategy to demonstrate the multiplexes analysis of adenosine and adenosine deaminase (ADA). This strategy allows for simple tethering of the specific oligonucleotides stabilizing the AgNCs to the nucleic acid probes. This is a new concept for aptasensors, and opens an opportunity for design of more novel biosensors based on the kissing complexes-induced strategy. Copyright © 2015 Elsevier B.V. All rights reserved.

  10. The HADES RV Programme with HARPS-N@TNG. III. Flux-flux and activity-rotation relationships of early-M dwarfs

    CERN Document Server

    Maldonado, J; Stelzer, B; Biazzo, K; Lanza, A F; Maggio, A; Micela, G; González-Álvarez, E; Affer, L; Claudi, R U; Cosentino, R; Damasso, M; Desidera, S; Hernández, J I González; Gratton, R; Leto, G; Messina, S; Molinari, E; Pagano, I; Perger, M; Piotto, G; Rebolo, R; Ribas, I; Sozzetti, A; Mascareño, A Suárez; Sanchez, R Zanmar

    2016-01-01

    (Abridged) Understanding stellar activity in M dwarfs is crucial for the physics of stellar atmospheres as well as for ongoing radial velocity exoplanet programmes. Despite the increasing interest in M dwarfs, our knowledge of the chromospheres of these stars is far from being complete. We aim to test whether the relations between activity, rotation, and stellar parameters and flux-flux relationships also hold for early-M dwarfs on the main-sequence. We analyse in an homogeneous and coherent way a well defined sample of 71 late-K/early-M dwarfs that are currently being observed in the framework of the HArps-n red Dwarf Exoplanet Survey (HADES). Rotational velocities are derived using the cross-correlation technique while emission flux excesses in the Ca II H & K and Balmer lines from Halpha up to Hepsilon are obtained by using the spectral subtraction technique. The relationships between the emission excesses and the stellar parameters are studied. Relations between pairs of fluxes of different chromosphe...

  11. Contribution of space platforms to a ground and airborne remote-sensing programme over active Italian volcanoes

    Science.gov (United States)

    Cassinis, R.; Lechi, G. M.; Tonelli, A. M.

    1974-01-01

    ERTS-1 imagery of the volcanic areas of southern Italy was used primarily for the evaluation of space platform capabilties in the domains of regional geology, soil and rock-type classification and, more generally, to study the environment of active volcanoes. The test sites were selected and equipped primarily to monitor thermal emission, but ground truth data was also collected in other domains (reflectance of rocks, soils and vegetation). The test areas were overflown with a two channel thermal scanner, while a thermo camera was used on the ground to monitor the hot spots. The primary goal of this survey was to plot the changes in thermal emission with time in the framework of a research program for the surveillance of active volcanoes. However, another task was an evaluation of emissivity changes by comparing the outputs of the two thermal channels. These results were compared with the reflectance changes observed on multispectral ERTS-1 imagery.

  12. Photovoltaic programme - edition 2003

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2003-07-01

    This publication issued by the Swiss Federal Office of Energy's Photovoltaics (PV) Programme presents an overview (in English) of activities and projects in the photovoltaics research and pilot and demonstration area in Switzerland. Progress in the area of future solar cell technologies, modules and building integration, system technologies, planning and operating aids is summarised. Also, PV for applications in developing countries, thermo-photovoltaics and international co-operation are commented on. In the area of pilot and demonstration projects, component development, PV integration in sloping roofs, on flat roofs and noise barriers as well as further PV plant are looked at. Also, measurement campaigns, studies, statistics and further PV-related topics are summarised. This volume also presents the abstracts of reports made by the project managers of 73 research and pilot and demonstration projects in these areas for 2002.

  13. Q36R polymorphism of KiSS-1 gene in Brazilian head and neck cancer patients.

    Science.gov (United States)

    Ruiz, Mariângela Torreglosa; Galbiatti, Ana Lívia Silva; Pavarino, Erika Cristina; Maniglia, José Victor; Goloni-Bertollo, Eny Maria

    2012-05-01

    The KiSS-1 metastasis-suppressor gene (KiSS-1) product (metastin, kisspeptin) is reported to act after binding with the natural ligand of a G-protein coupled receptor and this gene product inhibits chemotaxis, invasion, and metastasis of cells. The aim of this study was to evaluate the Q36R polymorphism of KiSS-1 in patients with head and neck cancer and to compare the results with healthy individuals and its association with clinicopathological parameters. Gender, age, smoking and alcohol consumption were analyzed for 744 individual (252 head and neck cancer patients and in 522 control individuals). The molecular analysis of these individuals was made after extraction of genomic DNA using the SSCP-PCR technique. This study did not reveal any significant differences in genotype frequencies between healthy individuals and patients with head and neck cancer or with the clinical parameters. This study showed an increase frequency of the Q36R polymorphism in pharyngeal cancer.

  14. Novel FGFR1 and KISS1R Mutations in Chinese Kallmann Syndrome Males with Cleft Lip/Palate

    Directory of Open Access Journals (Sweden)

    Hao Xu

    2015-01-01

    Full Text Available Kallmann syndrome (KS is characterized by isolated hypogonadotropic hypogonadism (IHH with anosmia and is sometimes associated with cleft lip/palate (CLP. In order to describe the clinical features, genetic etiology, and treatment outcome of KS males with CLP, we performed genetic screening for 15 known causal IHH genes (KAL1, FGFR1, NELF, FGF8, CHD7, WDR11, SEMA3A, KISS1R, KISS1, PROKR2, PROK2, TAC3, TACR3, GNRH1, and GNRHR in four KS with CLP patients and six IHH patients without CLP. Two novel heterozygous missense mutations in FGFR1, (NM_001174066: c.776G>A (p.G259E and (NM_001174066: c.358C>T (p.R120C, were identified in a 23-year-old KS male with cleft lip and an 18-year-old KS patient with cleft lip and palate, dental agenesis, and high arched palate, respectively. These two mutations were not presented in their healthy parents and 200 normal controls. One novel heterozygous missense mutation in KISS1R, (NM_032551: c.587C>A (p.P196H, was identified in an 18-year-old KS male with cleft lip and dental agenesis who developed sperm after being treated with gonadotropin. This mutation was also presented in his healthy father and grandfather. These results have implications for the diagnosis, genetic counseling, and treatment of KS and CLP males with mutations in FGFR1 gene.

  15. Kissing iliac artery stent technique for salvage of a total occlusion of a jailed common iliac artery.

    Science.gov (United States)

    Ishizuka, Shuichi; Habara, Maoto; Nasu, Kenya

    2014-03-01

    A 70-year-old female was admitted to our hospital due to claudication of the left leg. The patient was diagnosed with peripheral artery disease and received endovascular therapy (EVT) with a stent implanted in the right common iliac artery (CIA) at another hospital 3 months earlier. The left CIA was jailed by the stent. We performed EVT for the chronic total occlusion (CTO) of the jailed left CIA. A kissing-stent strategy was selected because the strut could not be fully opened. The wire was crossed through the stent strut, since passing the wire outside of the stent was problematic. A balloon was dilated at the stent strut and further inserted while dilated in order to create a space between the implanted stent and opposite aorta wall. Finally, a wire was successfully crossed outside of the stent in this space. A balloon-expandable stent was implanted at the aorta to left CIA in order to perform the kissing-stent technique. Additionally, a self-expandable stent was deployed at the left external iliac artery. To facilitate kissing-stent technique for a jailed CIA CTO lesion, inserting the balloon while inflated at the bifurcation was useful to create space for advancing the guidewire along the stent.

  16. "Kissing the crone": on a Slavic and pre-Slavic mythological figure

    Directory of Open Access Journals (Sweden)

    Katja Hrobat Virgolet

    2012-07-01

    Full Text Available The research focuses on the folklore regarding two monolith ‘Babas’ in the Karst region of Slovenia. From the Karst to the Vipava Valley and Croatian Istria, parents used the ‘baba’ to frighten their children, telling them that they would have to kiss or blow up the buttocks of the ugly ‘old baba’ (crone’ or swallow her snivel on their _rst visit to a neighbouring town (for example, Trieste. Throughout the area, stone ‘Babas’ were represented as personifications of a repulsive old woman. At Grobnik in Croatian Istria, a ‘Baba’ with pronounced female attributes is carved in rock at the entrance to the old town. In some parts, the imaginary or stone ‘Babas’ were given offerings of crops. At Golec in Cicarija, a three-day ritual to ‘Baba’ was performed on Midsummer Day, offering her water, soil and ashes (from a bonfire. Immolation and rituals performed in the immediate vicinity of ‘Babas’ are recorded also in Macedonia, as well as in France and Italy. The ‘Baba’ appears to be an omnipresent, yet fragmentarily preserved phenomenon in the traditions of all Slavic peoples. It encompasses different phenomena. On the one hand it represents old age, infertility, and on the other young, fertile things, or a structural support or basis. In Slavic traditions, the ‘baba’ is connected with water or equated with precipitation phenomena (rain, hail, places where storms break, etc.. She is associated with moisture through some adjectives (‘snotty’, ‘muddy’ or through the location of the stone monoliths by the water. According to the Rodik tradition, the ‘baba’s’ urine turns to rain, her fart to wind, and by lifting her skirt she brings clear weather. Slavic and Indo-European folklore material points to a close connection between bodily winds and conception, birth, and new souls. The lifting of the skirt is reminiscent of the obscene gesture by Baubó in Greek mythology. In several rituals of the Balkans

  17. Positive affect as coercive strategy: conditionality, activation and the role of psychology in UK government workfare programmes.

    Science.gov (United States)

    Friedli, Lynne; Stearn, Robert

    2015-06-01

    Eligibility for social security benefits in many advanced economies is dependent on unemployed and underemployed people carrying out an expanding range of job search, training and work preparation activities, as well as mandatory unpaid labour (workfare). Increasingly, these activities include interventions intended to modify attitudes, beliefs and personality, notably through the imposition of positive affect. Labour on the self in order to achieve characteristics said to increase employability is now widely promoted. This work and the discourse on it are central to the experience of many claimants and contribute to the view that unemployment is evidence of both personal failure and psychological deficit. The use of psychology in the delivery of workfare functions to erase the experience and effects of social and economic inequalities, to construct a psychological ideal that links unemployment to psychological deficit, and so to authorise the extension of state-and state-contracted-surveillance to psychological characteristics. This paper describes the coercive and punitive nature of many psycho-policy interventions and considers the implications of psycho-policy for the disadvantaged and excluded populations who are its primary targets. We draw on personal testimonies of people experiencing workfare, policy analysis and social media records of campaigns opposed to workfare in order to explore the extent of psycho-compulsion in workfare. This is an area that has received little attention in the academic literature but that raises issues of ethics and professional accountability and challenges the field of medical humanities to reflect more critically on its relationship to psychology.

  18. Kiss-1及基质金属蛋白酶9在结肠癌组织中的表达及意义%Expression and significance of Kiss-1 and matrix metalloproteinase-9 in colonic cancer

    Institute of Scientific and Technical Information of China (English)

    李旺林; 曹杰; 杜洪; 张伟健; 王辉; 杨平; 谭明华

    2007-01-01

    目的 检测Kiss-1及基质金属蛋白酶9(MMP-9)在结肠癌组织中的表达,探讨其临床意义.方法 采用链霉菌抗生物素蛋白-过氧化酶连接(SP)法检测68例结肠癌,26例结肠腺瘤及26例正常结肠组织中Kiss-1及MMP-9的表达情况,分析其与各种临床病理参数的关系及两者表达的关联性.结果 Kiss-1蛋白metastin在结肠癌组中的表达率(48.5%)明显低于结肠腺瘤组(80.8%)及正常结肠组(96.2%),并且其在结肠癌组中的表达率在不同的组织学分级、肌层浸润深度及淋巴结转移中差异有统计学意义(P均<0.05).MMP-9在结肠癌组中的表达率(75%)明显高于结肠腺瘤组(38.5%)和正常结肠组(15.4%),其在结肠癌组中的表达率在不同的肌层浸润深度、淋巴转移中差异有统计学意义(P均<0.05).Kiss-1蛋白metastin与MMP-9在结肠癌中的表达呈相反的趋势,有关联性(P<0.01).结论 Kiss-1蛋白metastin的表达下调和MMP-9的表达上调可能与结肠癌的浸润、转移有关.

  19. The Kissing-Loop T-Shaped Structure Translational Enhancer of Pea Enation Mosaic Virus Can Bind Simultaneously to Ribosomes and a 5′ Proximal Hairpin

    Science.gov (United States)

    Gao, Feng; Gulay, Suna P.; Kasprzak, Wojciech; Dinman, Jonathan D.

    2013-01-01

    The Pea Enation Mosaic Virus (PEMV) 3′ translational enhancer, known as the kissing-loop T-shaped structure (kl-TSS), binds to 40S subunits, 60S subunits, and 80S ribosomes, whereas the Turnip crinkle virus (TCV) TSS binds only to 60S subunits and 80S ribosomes. Using electrophoretic mobility gel shift assay (EMSA)-based competition assays, the kl-TSS was found to occupy a different site in the ribosome than the P-site-binding TCV TSS, suggesting that these two TSS employ different mechanisms for enhancing translation. The kl-TSS also engages in a stable, long-distance RNA-RNA kissing-loop interaction with a 12-bp 5′-coding-region hairpin that does not alter the structure of the kl-TSS as revealed by molecular dynamics simulations. Addition of the kl-TSS in trans to a luciferase reporter construct containing either wild-type or mutant 5′ and 3′ PEMV sequences suppressed translation, suggesting that the kl-TSS is required in cis to function, and both ribosome-binding and RNA interaction activities of the kl-TSS contributed to translational inhibition. Addition of the kl-TSS was more detrimental for translation than an adjacent eIF4E-binding 3′ translational enhancer known as the PTE, suggesting that the PTE may support the ribosome-binding function of the kl-TSS. Results of in-line RNA structure probing, ribosome filter binding, and high-throughput selective 2′-hydroxyl acylation analyzed by primer extension (hSHAPE) of rRNAs within bound ribosomes suggest that kl-TSS binding to ribosomes and binding to the 5′ hairpin are compatible activities. These results suggest a model whereby posttermination ribosomes/ribosomal subunits bind to the kl-TSS and are delivered to the 5′ end of the genome via the associated RNA-RNA interaction, which enhances the rate of translation reinitiation. PMID:23986599

  20. Drosophila Grainyhead specifies late programmes of neural proliferation by regulating the mitotic activity and Hox-dependent apoptosis of neuroblasts.

    Science.gov (United States)

    Cenci, Caterina; Gould, Alex P

    2005-09-01

    The Drosophila central nervous system is generated by stem-cell-like progenitors called neuroblasts. Early in development, neuroblasts switch through a temporal series of transcription factors modulating neuronal fate according to the time of birth. At later stages, it is known that neuroblasts switch on expression of Grainyhead (Grh) and maintain it through many subsequent divisions. We report that the function of this conserved transcription factor is to specify the regionalised patterns of neurogenesis that are characteristic of postembryonic stages. In the thorax, Grh prolongs neural proliferation by maintaining a mitotically active neuroblast. In the abdomen, Grh terminates neural proliferation by regulating the competence of neuroblasts to undergo apoptosis in response to Abdominal-A expression. This study shows how a factor specific to late-stage neural progenitors can regulate the time at which neural proliferation stops, and identifies mechanisms linking it to the Hox axial patterning system.

  1. Evaluating an in-home multicomponent cognitive behavioural programme to manage concerns about falls and associated activity avoidance in frail community-dwelling older people: Design of a randomised control trial [NCT01358032

    Directory of Open Access Journals (Sweden)

    van Rossum Erik

    2011-09-01

    Full Text Available Abstract Background Concerns about falls are frequently reported by older people. These concerns can have serious consequences such as an increased risk of falls and the subsequent avoidance of activities. Previous studies have shown the effectiveness of a multicomponent group programme to reduce concerns about falls. However, owing to health problems older people may not be able to attend a group programme. Therefore, we adapted the group approach to an individual in-home programme. Methods/Design A two-group randomised controlled trial has been developed to evaluate the in-home multicomponent cognitive behavioural programme to manage concerns about falls and associated activity avoidance in frail older people living in the community. Persons were eligible for study if they were 70 years of age or over, perceived their general health as fair or poor, had at least some concerns about falls and associated avoidance of activity. After screening for eligibility in a random sample of older people, eligible persons received a baseline assessment and were subsequently allocated to the intervention or control group. Persons assigned to the intervention group were invited to participate in the programme, while those assigned to the control group received care as usual. The programme consists of seven sessions, comprising three home visits and four telephone contacts. The sessions are aimed at instilling adaptive and realistic views about falls, as well as increasing activity and safe behaviour. An effect evaluation, a process evaluation and an economic evaluation are conducted. Follow-up measurements for the effect evaluation are carried out 5 and 12 months after the baseline measurement. The primary outcomes of the effect evaluation are concerns about falls and avoidance of activity as a result of these concerns. Other outcomes are disability and falls. The process evaluation measures: the population characteristics reached; protocol adherence by

  2. Cigotica programme: pediatric experiences

    Directory of Open Access Journals (Sweden)

    Lešović Snežana

    2011-01-01

    Full Text Available Introduction The alarming spread of obesity epidemic in children and adolsecents, as well as the absence of tested and efficient measures and programmes on obesity preven­tion indicate the necessity for the establishment of the Centre for the prevention, treatment and rehabilitation of obesity in children and adolescents and the 'Cigotica Programme' at the Special Hospital 'Zlatibor'. The advantage of the 'Cigotica' Programme is the multidisciplinary approach to treating obese children, which implies specific education, dietetic interventions with the reduction in the total daily calorie intake, physical activity, medical, educational and psychological support, change of behavior and lifestyle. Objective To define obesity complications, metabolic risk factors and treatment effects on body composition and metabolic parameters in adolescents participating in the 'Cigotica' Programme. Method 1,030 adolescents were examined (498 girls and 532 boys, aged 12 to 18, average age 15.45, diagnosed with primary obesity, hospitalized at the Centre for the prevention, treatment and rehabilitation of obesity in children and adolescents at the Special Hospital 'Zlatibor', in the period from 27/07/2008 to 03/10/2010. Hospitalization lasted 21 days. Obesity criterion was body mass index (BMI > +2 SD . Body The Special Hospital for the Thyroid Gland and Metabolism Zlatibor mass, BMI, % of fat were obtained by means of Tanita scales for determining body composition using the impendence method. Apart from medical examination, blood pressure was also taken. The levels of triglycerides, total HDL and LDL cholesterols, uric acids and glycemia were determined on the second and twenty-first day of hospitalization after a 12-day fasting period. Results After the multidisciplinary treatment, the average reduction in body mass (p< 0.05 in all adolescents was 5.92 ± 2.71 kg, in boys - 6.24 ±3.24 kg, and in girls -5.86±2.4. During the 21-day hospitalization, the average

  3. An After-School, high-intensity, interval physical activity programme improves health-related fitness in children

    Directory of Open Access Journals (Sweden)

    Sergio Reloba Martínez

    Full Text Available Abstract Health problems related to a low level of physical activity (PA in children and adolescents have prompted research into extracurricular PA programs. This study was designed to determine the effects of two different levels of PA on the health-related fitness of school children. Ninety-four girls and boys (7-9 years were randomly assigned to a control group (CG or intervention group (IG. Over a 12 week study period, children in the CG participated in a similar PA program to that of a standard school physical education program while those in the IG completed a high intensity interval training (HIIT program. Both programs involved two 40 minute extracurricular sessions per week. Our findings indicate that the HIIT intervention improved motor capacity (speed/agility, Vpeak, VO2 max and excess post-exercise oxygen consumption (EPOC (p < 0.05 along with the musculoskeletal capacity of the lower trunk (mean propulsive velocity and standing long jump, p < 0.05. The PA program had no effect on anthropometric variables or hand-grip strength. The data indicate that a 12 week strength training program using workloads adapted to children may significantly improve several markers of health and physical fitness compared to a standard school PA program.

  4. Effects of physical activity programmes in the workplace (PAPW) on the perception and intensity of musculoskeletal pain experienced by garment workers.

    Science.gov (United States)

    Pereira, Cynara Cristina Domingues Alves; López, Ramón Fabian Alonso; Vilarta, Roberto

    2013-01-01

    The physical activity programmes in the workplace (PAPW) are applied to minimize the prevalence, incidence and intensity of pain. This study evaluated the perception of pain and quantifies its intensity among garment workers before and after performing a PAPW. We included 61 workers of a clothing company, who were classified randomly into experimental group (n = 44) 28.7 ± 8.8 years old and a control group (n = 17) 27.8 ± 7.4 years (20-43 years). The Trigger Points test questionnaire was used to assess pain perception and quantify its intensity. The PAPW was conducted in 15-minute sessions per day, consisting of stretching exercises (40%), muscular endurance (40%), self-massage relaxation and massage techniques (10%), and group dynamics (10%). The garment workers who participated in the PAPW showed a significant reduction of pain felt in the neck and wrists, and also a reduction in pain intensity in shoulders, arms, fingers and wrists that are most often strained during sewing. Our findings suggest that PAPW that target muscle groups that are more tense in sewing tasks, may be considered by companies for supporting adaptation to the work environment and improving health by reducing muscle and joint pain.

  5. A Randomised Control Trial of the Impact of a Computer-Based Activity Programme upon the Fitness of Children with Autism

    Directory of Open Access Journals (Sweden)

    Kathleen Dickinson

    2014-01-01

    Full Text Available The poor levels of fitness in children with autism are prompting concern for the children’s future health. This study looked to assess if a computer-based activity programme could improve fitness levels (as reflected in cardiopulmonary function of these children, and achieve a reduction in their body mass index. In a randomised controlled trial, 50 children with autism (of which 33 were under the age of 11 years and 39 were boys were allocated to an intervention group which encouraged them to use the Nintendo Wii and the software package “Mario and Sonics at the Olympics” in addition to their routine physical education classes. 50 children with autism (34 under the age of 11 years and 40 being boys acted as controls. At the end of one year, analysis of the changes in scores using analysis of covariance (ANCOVA on the Eurofit fitness tests showed that the intervention group had made statistically significant improvement on all tests other than flexibility. These improvements were also significantly better than controls. This type of intervention appears to be an effective addition to standard fitness training in order to help children with autism improve their fitness levels.

  6. Programme impact on current contraception in Bangladesh.

    Science.gov (United States)

    Latif, M A

    1994-03-01

    "This paper analyses the impact of three credit programmes--the Bangladesh Rural Advancement Committee (BRAC), the Bangladesh Rural Development Board's Rural Development-12 (BRDB RD-12), and the Grameen Bank (GB), on current rate of contraception. These programmes are targeted to alleviate poverty by providing group-based credit to the rural poor in creating self employment opportunities. With small credits, these programmes combine family planning activities in terms of consciousness raising, awareness building and motivation. Sample survey data are used to analyse the problem of impact evaluation. The analyses show that the BRAC and the GB programmes have [a] significantly positive impact on the current rate of contraception, while the BRDB RD-12 programme does not have any such impact. It is also found that education, both of female[s] and male[s] separately, and child survivorship have independently positive impact[s] on current contraception."

  7. A Comparison of the Detectability of Dry Contact Kissing Bonds in Adhesive Joints Using Longitudinal, Shear and High Power Ultrasonic Techniques

    Science.gov (United States)

    Brotherhood, C. J.; Drinkwater, B. W.; Guild, F. J.

    2003-03-01

    This paper details a study on the detectability of dry contact kissing bonds in adhesive joints using three ultrasonic inspection techniques. Conventional normal incidence longitudinal and shear wave inspection were conducted on dry contact kissing bonds using a standard immersion transducer and an EMAT respectively. The detectability of the dry contact kissing bonds was assessed by calculating the reflection coefficient of the interface at varying loads for a number of surface roughnesses. A high power ultrasonic method was also employed to determine the non-linear behavior of the adhesive interface. The non-linearity of the interface was determined by the ratio of the amplitudes of the first harmonic and fundamental frequencies of the transmitted waveform. It was found that the high power technique showed the greatest sensitivity to kissing bonds at low contact pressures, however at high loads conventional longitudinal wave testing was more sensitive. It was also noted that a combination of two or more techniques could provide enhanced information about the kissing bond compared to a single technique alone.

  8. A proposed adaptation of the European Foundation for Quality Management Excellence Model to physical activity programmes for the elderly - development of a quality self-assessment tool using a modified Delphi process

    Directory of Open Access Journals (Sweden)

    Marques Ana I

    2011-09-01

    Full Text Available Abstract Background There has been a growing concern in designing physical activity (PA programmes for elderly people, since evidence suggests that such health promotion interventions may reduce the deleterious effects of the ageing process. Complete programme evaluations are a necessary prerequisite to continuous quality improvements. Being able to refine, adapt and create tools that are suited to the realities and contexts of PA programmes for the elderly in order to support its continuous improvement is, therefore, crucial. Thus, the aim of this study was to develop a self-assessment tool for PA programmes for the elderly. Methods A 3-round Delphi process was conducted via the Internet with 43 national experts in PA for the elderly, management and delivery of PA programmes for the elderly, sports management, quality management and gerontology, asking experts to identify the propositions that they considered relevant for inclusion in the self-assessment tool. Experts reviewed a list of proposed statements, based on the criteria and sub-criteria from the European Foundation for Quality Management Excellence Model (EFQM and PA guidelines for older adults and rated each proposition from 1 to 8 (disagree to agree and modified and/or added propositions. Propositions receiving either bottom or top scores of greater than 70% were considered to have achieved consensus to drop or retain, respectively. Results In round 1, of the 196 originally-proposed statements (best practice principles, the experts modified 41, added 1 and achieved consensus on 93. In round 2, a total of 104 propositions were presented, of which experts modified 39 and achieved consensus on 53. In the last round, of 51 proposed statements, the experts achieved consensus on 19. After 3 rounds of rating, experts had not achieved consensus on 32 propositions. The resulting tool consisted of 165 statements that assess nine management areas involved in the development of PA programmes for

  9. SET-Routes programme

    CERN Document Server

    CERN audiovisual service

    2009-01-01

    The SET-Routes programme, launched in 2007 with the goal of attracting girls and young women to careers in science, came to an end in April this year. The result of a collaboration between EMBL, EMBO and CERN, the programme established a network of "ambassadors", women scientists who went out to talk about their careers in science at schools and universities across Europe.

  10. Programmable Logic Controllers.

    Science.gov (United States)

    Insolia, Gerard; Anderson, Kathleen

    This document contains a 40-hour course in programmable logic controllers (PLC), developed for a business-industry technology resource center for firms in eastern Pennsylvania by Northampton Community College. The 10 units of the course cover the following: (1) introduction to programmable logic controllers; (2) DOS primer; (3) prerequisite…

  11. Metal Abundances of KISS Galaxies. V. Nebular Abundances of Fifteen Intermediate Luminosity Star-Forming Galaxies

    CERN Document Server

    Hirschauer, Alec S; Bresolin, Fabio; Saviane, Ivo; Yegorova, Irina

    2015-01-01

    We present high S/N spectroscopy of 15 emission-line galaxies (ELGs) cataloged in the KPNO International Spectroscopic Survey (KISS), selected for their possession of high equivalent width [O III] lines. The primary goal of this study was to attempt to derive direct-method ($T_e$) abundances for use in constraining the upper-metallicity branch of the $R_{23}$ relation. The spectra cover the full optical region from [O II]{\\lambda}{\\lambda}3726,3729 to [S III]{\\lambda}{\\lambda}9069,9531 and include the measurement of [O III]{\\lambda}4363 in 13 objects. From these spectra, we determine abundance ratios of helium, nitrogen, oxygen, neon, sulfur, and argon. We find these galaxies to predominantly possess oxygen abundances in the range of 8.0 $\\lesssim$ 12+log(O/H) $\\lesssim$ 8.3. We present a comparison of direct-method abundances with empirical SEL techniques, revealing several discrepancies. We also present a comparison of direct-method oxygen abundance calculations using electron temperatures determined from e...

  12. Retrospektive Bestimmung der elektromagnetischen Exposition durch analoge Rundfunksender im Rahmen von KiSS

    Directory of Open Access Journals (Sweden)

    J. Philipp

    2007-06-01

    Full Text Available Im Rahmen einer epidemiologischen Fall-Kontroll-Studie zur Untersuchung eines möglichen Zusammenhangs zwischen der Häufigkeit kindlicher Leukämien und elektromagnetischer Strahlung (,,KiSS" – Kindliche Leukämien und Expositionen in der Umgebung von hochfrequenten Sendestationen soll die Exposition durch leistungsstarke analoge Rundfunksender retrospektiv (Zeitraum 1982–2003 quantifiziert werden. Die zu betrachtenden Sendernetze für AM-Hörfunk, FM-Hörfunk und analoges Fernsehen unterscheiden sich nicht nur hinsichtlich der Modulationsart und der von den Einzelsendern abgestrahlten Leistung, sondern auch in der Netzkonfiguration und den je nach Wellenbereich verschiedenen Strahlungseigenschaften der Sendeantennen. Damit sind bei diesen drei Rundfunkdiensten sowohl die absolute Größe als auch die räumliche Struktur der hervorgerufenen Exposition verschieden. Es wird dargelegt, wie die für die Prognose der Rundfunkversorgung verwendeten Rechenverfahren zur Modellierung der Feldstärke für die Modellierung der Exposition herangezogen und durch Kontrollmessungen validiert werden und wie trotz Wahrung der Vertraulichkeit der dabei unabdingbar zu verwendenden Senderbetriebsdaten eine unerwünschte Beeinflussung der Studienergebnisse durch die Senderbetreiber ausgeschlossen wird.

  13. Hepatitis C virus genomic RNA dimerization is mediated via a kissing complex intermediate

    Science.gov (United States)

    Shetty, Sumangala; Kim, Seungtaek; Shimakami, Tetsuro; Lemon, Stanley M.; Mihailescu, Mihaela-Rita

    2010-01-01

    With over 200 million people infected with hepatitis C virus (HCV) worldwide, there is a need for more effective and better-tolerated therapeutic strategies. The HCV genome is a positive-sense; single-stranded RNA encoding a large polyprotein cleaved at multiple sites to produce at least ten proteins, among them an error-prone RNA polymerase that confers a high mutation rate. Despite considerable overall sequence diversity, in the 3′-untranslated region of the HCV genomic RNA there is a 98-nucleotide (nt) sequence named X RNA, the first 55 nt of which (X55 RNA) are 100% conserved among all HCV strains. The X55 region has been suggested to be responsible for in vitro dimerization of the genomic RNA in the presence of the viral core protein, although the mechanism by which this occurs is unknown. In this study, we analyzed the X55 region and characterized the mechanism by which it mediates HCV genomic RNA dimerization. Similar to a mechanism proposed previously for the human immunodeficiency 1 virus (HIV-1) genome, we show that dimerization of the HCV genome involves formation of a kissing complex intermediate, which is converted to a more stable extended duplex conformation in the presence of the core protein. Mutations in the dimer linkage sequence loop sequence that prevent RNA dimerization in vitro significantly reduced but did not completely ablate the ability of HCV RNA to replicate or produce infectious virus in transfected cells. PMID:20360391

  14. Kissing molars: report of three cases and new prospective on aetiopathogenetic theories.

    Science.gov (United States)

    Menditti, Dardo; Laino, Luigi; Cicciù, Marco; Mezzogiorno, Antonio; Perillo, Letizia; Menditti, Marco; Cervino, Gabriele; Lo Muzio, Lorenzo; Baldi, Alfonso

    2015-01-01

    Kissing molars (KMs) is an extremely rare condition of impacted third molars, pointed in the opposite direction in a single follicular space; it consists exactly in a full impacted of permanent molars which occurs only in the lower jaw. Actually, about less than thirty cases have been reported in scientific literatures. The aetiology and pathogenesis of this pathological double dental inclusion remain unknown; above all events that lead two molars to appear, as KMs remain mysterious. The association to metabolic connective diseases such as mucopolysaccharidosis was emphasized. KMs considered as an isolated event, may be associated to an abnormal position of the tooth-bud from lower permanent molars, or fourth supernumerary tooth (distomolar). Recently, hyperplastic dental follicle (HDF), with a down regulation of matrix metal-proteinases and up regulation of several genes of collagens, has been mentioned in association with KMs. In this paper, after having analyzed three new cases of KMs that have been treated, we report a new hypothesis. This last is based on the failure in the dental follicle's ability to initiate or continue properly resorption of the overlying alveolar bone, by many exogenous factors which may act on eruptive phase that would lead to its rotation with its contents coming out a pathological situation of KMs. The therapy of choice is related to the surgical removal of KMs through a double odontectomy with transalveolar method. Other treatments can be, eventually, orthodontic therapy of the impacted teeth and a radiological follow-up without surgery.

  15. A process evaluation of a supervisory development programme

    Directory of Open Access Journals (Sweden)

    Jasper Buys

    2012-07-01

    Full Text Available Orientation: An important evaluation function is to assess the adequacy of the programme process, including the extent to which the appropriate people participate in its activities.Research purpose: The study aimed to provide information about coverage, service delivery, organisational resources, and medium-term outcomes for a supervisory development programme (SDP in the hospitality industry.Motivation for the study: The primary motivation was to assist programme staff to understand their programme and also to generate information that the programme staff could use to reflect on the programme’s performance and future direction.Research design, approach and method: A mixture of quantitative and qualitative methods was employed, namely: a literature review; personal interviews; and internal programme documentation, such as e-mail correspondence, financial statements, attendance registers, and personnel records. Personal interviews were conducted with two stakeholders, namely the programme manager and programme administrator. The programme data of 69 participants in the SDP were utilised for the evaluation.Main findings: The evaluation’s main finding concluded that the ‘implemented programme’ was not congruent with the ‘planned programme’.Practical/managerial implications: It is recommended that programme activities and theory should be re-evaluated, as the programme is used mainly as a training programme for new appointees, rather than a management development programme to create a pool of potential supervisors. Programme uptake and output should also be closely aligned. The length of time that people in the pool of potential supervisors had to wait before they were appointed should also be reduced.Contribution/value-add: By explicating the basic programme theory and studying programme implementation, this evaluation serves as a starting point for future evaluations of the SDP.

  16. Informationist programme in support of biomedical research: a programme description and preliminary findings of an evaluation.

    Science.gov (United States)

    Whitmore, Susan C; Grefsheim, Suzanne F; Rankin, Jocelyn A

    2008-06-01

    The informationist programme at the Library of the National Institutes of Health (NIH) in Bethesda, MD, USA has grown to 14 informationists working with 40 clinical and basic science research teams. This case report, intended to contribute to the literature on informationist programmes, describes the NIH informationist programme, including implementation experiences, the informationists' training programme, their job responsibilities and programme outcomes. The NIH informationist programme was designed to enhance the library's service capacity. Over time, the steps for introducing the service to new groups were formalized to ensure support by leadership, the team being served and the library. Job responsibilities also evolved from traditional library roles to a wide range of knowledge management activities. The commitment by the informationist, the team and the library to continuous learning is critical to the programme's success. RESULTS / OUTCOMES: NIH scientists reported that informationists saved them time and contributed to teamwork with expert searching and point-of-need instruction. Process evaluation helped refine the programme. High-level, preliminary outcomes were identified from a survey of scientists receiving informationist services, along with key informant interviews. Process evaluation examined service implementation, informationists' training and service components. Anecdotal evidence has also indicated a favourable response to the programme.

  17. Coronary bifurcation lesions treated with double kissing crush technique compared to classical crush technique: serial intravascular ultrasound analysis.

    Science.gov (United States)

    Shan, Shou-Jie; Ye, Fei; Liu, Zhi-Zhong; Tian, Nai-Liang; Zhang, Jun-Jie; Chen, Shao-Liang

    2013-04-01

    The double kissing (DK) crush technique is a modified version of the crush technique. It is specifically designed to increase the success rate of the final kissing balloon post-dilatation, but its efficacy and safety remain unclear. Data were obtained from the DKCRUSH-I trial, a prospective, randomized, multi-center study to evaluate safety and efficacy. Post-procedural and eight-month follow-up intravascular ultrasound (IVUS) analysis was available in 61 cases. Volumetric analysis using Simpson's method within the Taxus stent, and cross-sectional analysis at the five sites of the main vessel (MV) and three sites of the side branch (SB) were performed. Impact of the bifurcation angle on stent expansion at the carina was also evaluated. Stent expansion in the SB ostium was significantly less in the classical crush group ((53.81 ± 13.51)%) than in the DK crush group ((72.27 ± 11.46)%) (P = 0.04). For the MV, the incidence of incomplete crush was 41.9% in the DK group and 70.0% in the classical group (P = 0.03). The percentage of neointimal area at the ostium had a tendency to be smaller in the DK group compared with the classical group ((16.4 ± 19.2)% vs. (22.8 ± 27.1)%, P = 0.06). The optimal threshold of post-procedural minimum stent area (MSA) to predict follow-up minimum lumen area (MLA) kissing balloon inflation (FKBI) and had smaller optimal cutoff value of post-procedural MSA at the SB ostium.

  18. Why people co-produce within activation services: the necessity of motivation and trust – an investigation of selection biases in a municipal activation programme in the Netherlands

    NARCIS (Netherlands)

    Fledderus, J.; Honingh, M.E.

    2016-01-01

    Activation services that aim at re-employment of jobseekers often suffer from ‘creaming’, i.e. selecting those who have the best qualifications to re-enter the labour market. New ways of delivery, such as co-production, are supposed to be less subject to selection mechanisms. To analyse whether

  19. The kissing-loop motif is a preferred site of 5' leader recombination during replication of SL3-3 murine leukemia viruses in mice

    DEFF Research Database (Denmark)

    Lund, Anders Henrik; Mikkelsen, J G; Schmidt, J

    1999-01-01

    , and the upstream part of the 5' untranslated region, enabled us to map recombination sites, guided by distinct scattered nucleotide differences. In 30 of 44 analyzed sequences, recombination was mapped to a 33-nucleotide similarity window coinciding with the kissing-loop stem-loop motif implicated in dimerization...... of the diploid genome. Interestingly, the recombination pattern preference found in replication-competent viruses from T-cell tumors is very similar to the pattern previously reported for retroviral vectors in cell culture experiments. The data therefore sustain the hypothesis that the kissing loop, presumably...

  20. The European Framework Programme under way

    CERN Multimedia

    2007-01-01

    The new European Framework Programme - FP7 - has recently started and will offer various possibilities for CERN to participate in EU co-funded projects for research and technological development. In December 2006, the Council of the European Union (EU) formally adopted the 7th European Framework Programme for research, technological development and demonstration activities (FP7). FP7 started on 1 January 2007 and will cover the period 2007 to 2013. With a total budget of 50.5 B-Euros, FP7 is the largest Framework Programme in the history of the EU. FP7 consists of four major sub-programmes, referred to as 'Specific Programmes'. 'Cooperation' is focused on collaborative research and is divided into 10 research themes. 'Ideas' is a new EU programme for funding of frontier research in all fields of science. 'Capacities' aims at strengthening the research capacities in Europe. Finally, 'People' succeeds the previous Marie Curie Programmes and targets the development of Europe's human potential. On 22 December...

  1. The European Framework Programme under way

    CERN Multimedia

    2007-01-01

    The new European Framework Programme - FP7 - has recently started and will offer various possibilities for CERN to participate in EU co-funded projects for research and technological development. In December 2006, the Council of the European Union (EU) formally adopted the 7th European Framework Programme for research, technological development and demonstration activities (FP7). FP7 started on 1st January 2007 and will cover the period 2007 to 2013. With a total budget of 50.5 billion euros, FP7 is the largest Framework Programme in the history of the EU. FP7 consists of four major sub-programmes, referred to as 'Specific Programmes'. 'Cooperation' is focused on collaborative research and is divided into 10 research themes. 'Ideas' is a new EU programme for funding frontier research in all fields of science. 'Capacities' aims at strengthening the research capacities in Europe. Finally, 'People' succeeds the previous Marie Curie Programmes and targets the development of Europe's human potential. On 22 Decem...

  2. National and regional asthma programmes in Europe

    Directory of Open Access Journals (Sweden)

    Olof Selroos

    2015-09-01

    Full Text Available This review presents seven national asthma programmes to support the European Asthma Research and Innovation Partnership in developing strategies to reduce asthma mortality and morbidity across Europe. From published data it appears that in order to influence asthma care, national/regional asthma programmes are more effective than conventional treatment guidelines. An asthma programme should start with the universal commitments of stakeholders at all levels and the programme has to be endorsed by political and governmental bodies. When the national problems have been identified, the goals of the programme have to be clearly defined with measures to evaluate progress. An action plan has to be developed, including defined re-allocation of patients and existing resources, if necessary, between primary care and specialised healthcare units or hospital centres. Patients should be involved in guided self-management education and structured follow-up in relation to disease severity. The three evaluated programmes show that, thanks to rigorous efforts, it is possible to improve patients' quality of life and reduce hospitalisation, asthma mortality, sick leave and disability pensions. The direct and indirect costs, both for the individual patient and for society, can be significantly reduced. The results can form the basis for development of further programme activities in Europe.

  3. Photovoltaic programme, edition 2004

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    This comprehensive publication issued by the Swiss Federal Office of Energy's Photovoltaics (PV) Programme presents an overview (in English) of activities and projects in the photovoltaics research and pilot and demonstration areas in Switzerland for the year 2003. Progress in future solar cell technologies as well as in the area of modules, building integration and system technologies is summarised. Also, national and international co-operation and multi-national pilot and demonstration projects are commented on. Associated projects such as eco-balances for PV systems, forecasting and modelling tools as well as system monitoring tools are discussed. In the area of pilot and demonstration projects, component development, PV integration in sloping roofs, on flat roofs and on facades as well as further PV plant are looked at. Also, measurement campaigns, studies, statistics and other PV-related topics are summarised. This volume presents a list of 92 projects in the PV area including the appropriate Internet links and is completed with a collection of project abstracts.

  4. An O(n(5)) algorithm for MFE prediction of kissing hairpins and 4-chains in nucleic acids.

    Science.gov (United States)

    Chen, Ho-Lin; Condon, Anne; Jabbari, Hosna

    2009-06-01

    Efficient methods for prediction of minimum free energy (MFE) nucleic secondary structures are widely used, both to better understand structure and function of biological RNAs and to design novel nano-structures. Here, we present a new algorithm for MFE secondary structure prediction, which significantly expands the class of structures that can be handled in O(n(5)) time. Our algorithm can handle H-type pseudoknotted structures, kissing hairpins, and chains of four overlapping stems, as well as nested substructures of these types.

  5. Pile up management at the high-luminosity LHC and introduction to the crab-kissing concept

    Science.gov (United States)

    Fartoukh, Stéphane

    2014-11-01

    Upgrading the integrated performance of the LHC, while preserving the quality of the physics data delivered to the experiments, is a real challenge for the high luminosity LHC (HL-LHC). This paper will give an overview of the situation in terms of performance and so-called pile up density which directly impacts on the reconstruction efficiency of the primary vertices at the interaction point. Both the present HL-LHC baseline and its possible extension with the so-called crab-kissing scheme will be discussed in this context.

  6. FAST joins Breakthrough programme

    Science.gov (United States)

    Banks, Michael

    2016-11-01

    The 180m Five-hundred-meter Aperture Spherical radio Telescope (FAST) - the world's largest single-aperture radio receiver - has become part of the Breakthrough Listen programme, which launched in July 2015 to look for intelligent life beyond Earth.

  7. Programmable mechanical metamaterials

    NARCIS (Netherlands)

    Florijn, H.C.B.

    2016-01-01

    We present a novel strategy to overcome this limitation and create programmable me chanical metamaterials, where the response of a single structure is determined and can be changed by the amount of lateral confinement.

  8. Elukestva õppe programm : Erasmus+

    Index Scriptorium Estoniae

    2014-01-01

    Erasmus+ programm liidab senised koostööprogrammid „Euroopa elukestva õppe programm“, „Euroopa Noored“ ning Euroopa komisjoni rahvusvahelised kõrgharidusprogrammid. Elukestva õppe programmi 2013 kokkuvõte

  9. The HAWC GRB Programme

    Science.gov (United States)

    Lennarz, D.; Taboada, I.

    2016-10-01

    HAWC is a very-high-energy gamma-ray extensive air shower detector located in central Mexico at an altitude of 4,100 m above sea level. This contribution summarises recent results of the HAWC GRB programme.

  10. Fellows, Associates & Students Programmes

    CERN Document Server

    2005-01-01

    The present document reviews the CERN Fellows, Associates and Students Programmes emphasizing the developments since 2000, when the previous review was presented to the Scientific Policy Committee, Finance Committee and Council (CERN/2325), and makes proposals for the coming five years. In summary, it is proposed to â?¢ Simplify the payment scheme for the Paid Scientific Associates Programme, which will no longer depend on candidateâ??s home support and age; â?¢ Broaden the scope of the Fellowship Programme, in order to facilitate the recruitment of young graduates in computing and engineering. Age-related eligibility conditions and payment levels will be replaced with experience-based criteria; â?¢ Modify subsistence rates for the Doctoral and Technical Student Programme in order to harmonize CERNâ??s payment levels with those offered by other research establishments. This document is presented for discussion and recommendation by the Scientific Policy Committee and approval by the Council. Additiona...

  11. Visual Basic educational programme

    OpenAIRE

    Pranaitis, Arūnas

    2005-01-01

    Visual basic educational programme Informational Technologies has become such a popular subject that they are applied in all works of life. However, Informational Technologies are still rarely used in the lessons at school. There are such reasons of the mentioned issue: · Insufficient base of computers, · The old software and its disadvantages, · The lack of computerized educational programmes. The aim of the work was to prove that it is actual to create computerized educat...

  12. Motivation programmes of organizations

    OpenAIRE

    Pízová, Tereza

    2008-01-01

    The Bachelor Thesis "'Motivation Programmes of Organizations" focuses on an extremely important area within personnel management. Employee motivation is crucial to the effective operation of businesses. Motivation programmes assist in increasing and maintaining employee motivation and demonstrate an organization's interest in its employees. This piece is on one hand concerned with theoretical foundations of motivation, describing theories and concepts important to the area of human behaviour ...

  13. Punch Card Programmable Microfluidics

    OpenAIRE

    George Korir; Manu Prakash

    2014-01-01

    Small volume fluid handling in single and multiphase microfluidics provides a promising strategy for efficient bio-chemical assays, low-cost point-of-care diagnostics and new approaches to scientific discoveries. However multiple barriers exist towards low-cost field deployment of programmable microfluidics. Incorporating multiple pumps, mixers and discrete valve based control of nanoliter fluids and droplets in an integrated, programmable manner without additional required external component...

  14. The Gold Standard Programme

    DEFF Research Database (Denmark)

    Neumann, Tim; Rasmussen, Mette; Ghith, Nermin

    2013-01-01

    To evaluate the real-life effect of an evidence-based Gold Standard Programme (GSP) for smoking cessation interventions in disadvantaged patients and to identify modifiable factors that consistently produce the highest abstinence rates.......To evaluate the real-life effect of an evidence-based Gold Standard Programme (GSP) for smoking cessation interventions in disadvantaged patients and to identify modifiable factors that consistently produce the highest abstinence rates....

  15. Programmable Multi-Chip Module

    Science.gov (United States)

    Kautz, David; Morgenstern, Howard; Blazek, Roy J.

    2004-11-16

    A multi-chip module comprising a low-temperature co-fired ceramic substrate having a first side on which are mounted active components and a second side on which are mounted passive components, wherein this segregation of components allows for hermetically sealing the active components with a cover while leaving accessible the passive components, and wherein the passive components are secured using a reflow soldering technique and are removable and replaceable so as to make the multi-chip module substantially programmable with regard to the passive components.

  16. Systematic Review of Results of Kissing Stents in the Treatment of Aortoiliac Occlusive Disease.

    Science.gov (United States)

    Groot Jebbink, Erik; Holewijn, Suzanne; Slump, Cornelis H; Lardenoije, Jan-Willem; Reijnen, Michel M P J

    2017-07-01

    Endovascular treatment of aortoiliac occlusive disease (AIOD) involving the aortic bifurcation is challenging. The gold standard is open surgery with patency rates up to 90% at 5 years, but has considerable morbidity and mortality. The kissing stent (KS) technique was introduced as an alternative. The goal of this review is to give an overview of the current results and role of the KS technique in AIOD treatment. The Cochrane guidelines were used to assure a systematic method. A search query designed in the Scopus search interface was used to identify relevant studies. Abstracts from the search were screened against the inclusion and exclusion criteria. During full-text reading, methodological quality was scored using a critical review list tailored to the topic of AIOD. Thereafter, study data were extracted and pooled for further analysis. In total, 143 abstracts were retrieved using Scopus, 116 were rejected and 7 more were rejected after full-text screening. One study was included after cross referencing. Twenty-one studies presented 1,390 patients. Rutherford classification 1/2/3 was the indication in 76.2% of patients, and 48.4% of the lesions were classified as Trans-Atlantic Inter-Society Consensus C or D. The technical success rate was 98.7%, and the complication rate was 10.8%. Clinical improvement at 30 days was achieved in 89.9%. Primary patency at 12, 24, and 60 months was 89.3%, 78.6%, and 69.0%, respectively. KS treatment of AIOD yields acceptable mid-term results, with high technical success rates and mostly minor complications occur. The long-term patency cannot yet match that of open surgery, underlining the need for further research that provides insight into factors related to reocclusion. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Reducing musculoskeletal injury and concussion risk in schoolboy rugby players with a pre-activity movement control exercise programme: a cluster randomised controlled trial.

    Science.gov (United States)

    Hislop, Michael D; Stokes, Keith A; Williams, Sean; McKay, Carly D; England, Mike E; Kemp, Simon P T; Trewartha, Grant

    2017-08-01

    Injury risk in youth rugby has received much attention, highlighting the importance of establishing evidence-based injury reduction strategies. To determine the efficacy of a movement control exercise programme in reducing injuries in youth rugby players and to investigate the effect of programme dose on injury measures. In a cluster-randomised controlled trial, 40 independent schools (118 teams, 3188 players aged 14-18 years) were allocated to receive either the intervention or a reference programme, both of which were to be delivered by school coaches. The intervention comprised balance training, whole-body resistance training, plyometric training, and controlled rehearsal of landing and cutting manoeuvres. Time-loss (>24 hours) injuries arising from school rugby matches were recorded by coaches and medical staff. 441 time-loss match injuries (intervention, 233; control, 208) were reported across 15 938 match exposure-hours (intervention, 9083; control, 6855). Intention-to-treat results indicated unclear effects of trial arm on overall match injury incidence (rate ratio (RR)=0.85, 90% confidence limits 0.61 to 1.17), although clear reductions were evident in the intervention arm for concussion incidence (RR=0.71, 0.48 to 1.05). When trial arm comparisons were limited to teams who had completed three or more weekly programme sessions on average, clear reductions in overall match injury incidence (RR=0.28, 0.14 to 0.51) and concussion incidence (RR=0.41, 0.17 to 0.99) were noted in the intervention group. A preventive movement control exercise programme can reduce match injury outcomes, including concussion, in schoolboy rugby players when compared with a standardised control exercise programme, although to realise the greatest effects players should complete the programme at least three times per week. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted

  18. HADES RV Programme with HARPS-N at TNG . III. Flux-flux and activity-rotation relationships of early-M dwarfs

    Science.gov (United States)

    Maldonado, J.; Scandariato, G.; Stelzer, B.; Biazzo, K.; Lanza, A. F.; Maggio, A.; Micela, G.; González-Álvarez, E.; Affer, L.; Claudi, R. U.; Cosentino, R.; Damasso, M.; Desidera, S.; González Hernández, J. I.; Gratton, R.; Leto, G.; Messina, S.; Molinari, E.; Pagano, I.; Perger, M.; Piotto, G.; Rebolo, R.; Ribas, I.; Sozzetti, A.; Suárez Mascareño, A.; Zanmar Sanchez, R.

    2017-01-01

    Context. Understanding stellar activity in M dwarfs is crucial for the physics of stellar atmospheres and for ongoing radial velocity exoplanet programmes. Despite the increasing interest in M dwarfs, our knowledge of the chromospheres of these stars is far from being complete. Aims: We test whether the relations between activity, rotation, and stellar parameters and flux-flux relationships previously investigated for main-sequence FGK stars and for pre-main-sequence M stars also hold for early-M dwarfs on the main-sequence. Although several attempts have been made so far, here we analyse a large sample of stars undergoing relatively low activity. Methods: We analyse in a homogeneous and coherent way a well-defined sample of 71 late-K/early-M dwarfs that are currently being observed in the framework of the HArps-N red Dwarf Exoplanet Survey (HADES). Rotational velocities are derived using the cross-correlation technique, while emission flux excesses in the Ca ii H & K and Balmer lines from Hα up to Hɛ are obtained by using the spectral subtraction technique. The relationships between the emission excesses and the stellar parameters (projected rotational velocity, effective temperature, kinematics, and age) are studied. Relations between pairs of fluxes of different chromospheric lines (flux-flux relationships) are also studied and compared with the literature results for other samples of stars. Results: We find that the strength of the chromospheric emission in the Ca ii H & K and Balmer lines is roughly constant for stars in the M0-M3 spectral range. Although our sample is likely to be biased towards inactive stars, our data suggest that a moderate but significant correlation between activity and rotation might be present, as well as a hint of kinematically selected young stars showing higher levels of emission in the calcium line and in most of the Balmer lines. We find our sample of M dwarfs to be complementary in terms of chromospheric and X-ray fluxes with

  19. Nevo palpebral dividido - "kissing nevus": relato clínico-patológico e tratamento cirúrgico de três casos Divided nevus of the eyelid - kissing nevus: clinicopathological report and surgical treatment of three cases

    Directory of Open Access Journals (Sweden)

    Eduardo Ferrari Marback

    2002-03-01

    Full Text Available Objetivo: Relatar aspectos clínico-patológicos e tratamento cirúrgico em três casos de nevo palpebral dividido -"kissing nevus". Métodos: Revisão dos prontuários de três pacientes portadores de nevo palpebral dividido. Análise dos aspectos clínico-patológicos, das técnicas cirúrgicas e resultados do tratamento. Resultados: A cura das lesões com bom resultado estético e funcional foi conseguida em todos os casos. Conclusões: A exérese do nevo palpebral dividido, em mais de um tempo cirúrgico utilizando deslizamento de retalhos cutâneos palpebrais e, em um caso, transplante de pele da pálpebra superior contralateral foi curativa nos três casos estudados.Purpose: To describe the clinical and pathological aspects, surgical treatment and results of three cases of divided nevus -"kissing nevus"- of the eyelid. Methods: Review of the clinicopathological data and surgical techniques used to treat these patients. Results: Cure of the lesions with good esthetic and functional results were obtained in all cases. Conclusions: Surgical excision of the divided nevus of the eyelid in more than one surgical session using flaps from the adjacent skin, and in one case, a free skin graft from the contralateral superior lid, was curative in the studied three cases.

  20. Control programme for cystic echinococcosis in Uruguay

    Directory of Open Access Journals (Sweden)

    Pilar Irabedra

    2016-06-01

    Full Text Available Cystic echinococcosis is a highly endemic parasitic zoonosis that is present in the Southern Cone countries of America. For several decades, various prevention and control programmes have been implemented in different countries and regions, with varying results. In Uruguay, a new control programme was implemented in 2006 that employed new strategies for canine diagnosis and treatment, dog population control, diagnosis in humans, epidemiological surveillance, and health education, including community participation. The control programme in Uruguay addresses the control and surveillance of the disease from a holistic perspective based on Primary Health Care, which has strengthened the community’s participation in developing and coordinating activities in an interdisciplinary manner. Similarly, the control programme that is currently implemented is based on a risk-focused approach. The surveillance and control measures were focused on small villages and extremely poor urban areas. In this study, the strategies used and the results obtained from 2008-2013 are analysed and discussed.

  1. Control programme for cystic echinococcosis in Uruguay

    Science.gov (United States)

    Irabedra, Pilar; Ferreira, Ciro; Sayes, Julio; Elola, Susana; Rodríguez, Miriam; Morel, Noelia; Segura, Sebastian; dos Santos, Estela; Guisantes, Jorge A

    2016-01-01

    Cystic echinococcosis is a highly endemic parasitic zoonosis that is present in the Southern Cone countries of America. For several decades, various prevention and control programmes have been implemented in different countries and regions, with varying results. In Uruguay, a new control programme was implemented in 2006 that employed new strategies for canine diagnosis and treatment, dog population control, diagnosis in humans, epidemiological surveillance, and health education, including community participation. The control programme in Uruguay addresses the control and surveillance of the disease from a holistic perspective based on Primary Health Care, which has strengthened the community’s participation in developing and coordinating activities in an interdisciplinary manner. Similarly, the control programme that is currently implemented is based on a risk-focused approach. The surveillance and control measures were focused on small villages and extremely poor urban areas. In this study, the strategies used and the results obtained from 2008-2013 are analysed and discussed. PMID:27223652

  2. The CERN & Society programme launches its newsletter

    CERN Multimedia

    Matteo Castoldi

    2016-01-01

    The newsletter will be issued quarterly. Sign up to remain informed about the latest initiatives of the CERN & Society programme!    The CERN & Society programme encompasses projects in the areas of education and outreach, innovation and knowledge exchange, and culture and creativity that spread the CERN spirit of scientific curiosity for the inspiration and benefit of society. The programme is funded primarily by the CERN & Society Foundation, a charitable foundation established by CERN and supported by individuals, trusts, organisations and commercial companies. The projects are inspired or enabled by CERN but lie outside of the Laboratory’s specific research mandate. We especially want to help young talent from around the world to flourish in the future. The programme is now launching its newsletter, which will be issued quarterly. Everybody who wants to be informed about CERN & Society’s activities, stay up-to-date with its latest in...

  3. The UNESCO Bioethics Programme: a review.

    Science.gov (United States)

    Langlois, Adéle

    2014-01-01

    UNESCO's Bioethics Programme was established in 1993. In twenty years it has adopted three international declarations, on the human genome (1997), human genetic data (2003) and bioethics (2005); produced reports on a wide range of bioethics issues; and developed capacity building and public education programmes in bioethics. Yet UNESCO has sometimes struggled to assert its authority in the wider bioethics world. Some bioethicists have criticized the 2005 declaration and suggested that the World Health Organization might be better placed to advance bioethics. In 2011, after four years of debate, UNESCO decided not to draft a convention on human reproductive cloning, because consensus on the issue proved impossible. This article reviews the standard setting and capacity building activities of the UNESCO Bioethics Programme. While the Programme faces challenges common to most intergovernmental organizations, its achievements in expanding international law and building bioethics capacity should not be underestimated.

  4. Control programme for cystic echinococcosis in Uruguay.

    Science.gov (United States)

    Irabedra, Pilar; Ferreira, Ciro; Sayes, Julio; Elola, Susana; Rodríguez, Miriam; Morel, Noelia; Segura, Sebastian; Santos, Estela Dos; Guisantes, Jorge A

    2016-05-24

    Cystic echinococcosis is a highly endemic parasitic zoonosis that is present in the Southern Cone countries of America. For several decades, various prevention and control programmes have been implemented in different countries and regions, with varying results. In Uruguay, a new control programme was implemented in 2006 that employed new strategies for canine diagnosis and treatment, dog population control, diagnosis in humans, epidemiological surveillance, and health education, including community participation. The control programme in Uruguay addresses the control and surveillance of the disease from a holistic perspective based on Primary Health Care, which has strengthened the community's participation in developing and coordinating activities in an interdisciplinary manner. Similarly, the control programme that is currently implemented is based on a risk-focused approach. The surveillance and control measures were focused on small villages and extremely poor urban areas. In this study, the strategies used and the results obtained from 2008-2013 are analysed and discussed.

  5. The development and pilot testing of the Self-management Programme of Activity, Coping and Education for Chronic Obstructive Pulmonary Disease (SPACE for COPD

    Directory of Open Access Journals (Sweden)

    Apps LD

    2013-07-01

    Full Text Available Lindsay D Apps,1 Katy E Mitchell,1 Samantha L Harrison,1 Louise Sewell,1 Johanna E Williams,1 Hannah ML Young,1 Michael Steiner,1 Mike Morgan,1 Sally J Singh1,2 1National Institute of Health Research CLAHRC-LNR Pulmonary Rehabilitation Research Group, University Hospitals of Leicester NHS, Leicester, UK; 2Applied Research Centre, Health and Lifestyle Interventions, Coventry University, Coventry, UK Purpose: There is no independent standardized self-management approach available for chronic obstructive pulmonary disease (COPD. The aim of this project was to develop and test a novel self-management manual for individuals with COPD. Patients: Participants with a confirmed diagnosis of COPD were recruited from primary care. Methods: A novel self-management manual was developed with health care professionals and patients. Five focus groups were conducted with individuals with COPD (N = 24 during development to confirm and enhance the content of the prototype manual. The Self-management Programme of Activity, Coping and Education for Chronic Obstructive Pulmonary Disease (SPACE for COPD manual was developed as the focus of a comprehensive self-management approach facilitated by health care professionals. Preference for delivery was initial face-to-face consultation with telephone follow-up. The SPACE for COPD manual was piloted with 37 participants in primary care. Outcome measures included the Self-Report Chronic Respiratory Questionnaire, Incremental Shuttle Walk Test, and Endurance Shuttle Walking Test (ESWT; measurements were taken at baseline and 6 weeks. Results: The pilot study observed statistically significant improvements for the dyspnea domain of the Self-Report Chronic Respiratory Questionnaire and ESWT. Dyspnea showed a mean change of 0.67 (95% confidence interval 0.23–1.11, P = 0.005. ESWT score increased by 302.25 seconds (95% confidence interval 161.47–443.03, P < 0.001. Conclusion: This article describes the development and

  6. The INTEGRAL Core Observing Programme

    OpenAIRE

    Winkler, C; Gehrels, N.; Lund, N.; Schoenfelder, V.; Ubertini, P.

    1999-01-01

    The Core Programme of the INTEGRAL mission is defined as the portion of the scientific programme covering the guaranteed time observations for the INTEGRAL Science Working Team. This paper describes the current status of the Core Programme preparations and summarizes the key elements of the observing programme.

  7. KiSS-1基因在黄牛精子发生中的表达%Expression of KiSS-1 gene in bovine testis spermatogenesis

    Institute of Scientific and Technical Information of China (English)

    邵宝平; 王继卿; 罗玉柱; 王建林; 陈建龙; 柴尔青; 徐元青

    2011-01-01

    为探讨亲吻素在黄牛精子发生中的生理功能,并为家畜生殖生理学研究提供参考,运用免疫组织化学SABC染色法并采用Image Pro-Plus 6.0软件,对成年黄牛睾丸中KiSS-1的表达及分布特征进行了研究。结果显示,在成年黄牛睾丸的精原细胞、初级精母细胞、次级精母细胞、早期精子细胞及间质细胞中均发现KiSS-1阳性反应产物,其各阳性细胞积分光密度(IOD)值的大小顺序为IOD初级精母细胞〉IOD间质细胞〉IOD次级精母细胞〉IOD精原细胞〉IOD早期精子细胞,而阳性产物在晚期精子细胞、精子和支持细胞及睾丸间质血管上未被发现;在部分间质细胞周围可见大量的KiSS-1免疫阳性反应产物,而在其他阳性细胞周围未见此现象。结果证实,KiSS-1基因在黄牛精子发生中发挥着重要作用,尤其在精原细胞的有丝分裂和精母细胞的减数分裂中可能发挥着非常重要的作用,而且间质细胞具有向睾丸微环境中分泌KiSS-1的功能。%To explore the physiologic function of the kisspeptins in bovine spermatogenesis as well as provide reference to research reproductive physiology of livestock,kisspeptins-1(Kiss-1) expression characteristics in the testis of adult cattle were studied by using SABC immunohistochemical staining and image analysis software(Image Pro-Plus6.0).In result,the KiSS-1 immunopositive production were found in the spermatogonia,primary spermatocytes,secondary spermatocytes,early spermatids and interstitial cells of the adult bovine testes,with the order of the total optical density of the positive cells(IOD) value as IODprimary spermatocytes IODinterstitial cells IODsecondary spermatocytes〉IODspermatogonia 〉IODearly spermatids(P〈0.05).However,the production were not found in the late spermatids,sperm,sertoli cells and interstitial blood vessels of the adult cattle testes.The large KiSS-1 immunoreactivity production was seen around

  8. A flexible COME and KISS QDC and TDC read-out scheme for PMT, MAPD and diamond detector applications

    Energy Technology Data Exchange (ETDEWEB)

    Rost, Adrian [Technische Universitaet Darmstadt, Darmstadt (Germany); Collaboration: HADES-Collaboration

    2015-07-01

    A flexible COME and KISS Charge-to-Digital-Converter (QDC) and Time-to-Digital-Converter (TDC) read-out scheme will be presented which can be used in a wide range of read-out applications in high energy physics experiments. The focus is on a calorimeter detector read-out via photomultiplier tubes (PMTs) or via multi-pixel avalanche photo-diodes (MAPDs), as well as on diamond detectors for the HADES and CBM experiments at the future FAIR facility in Darmstadt. The detector input signals are integrated with the help of simple analogue electronics (KISS: Keep it Small and Simple). Afterwards the charge measurement is transformed into a Time-over-Threshold (ToT) measurement using an commercial (COME: Use Commercial Elements) FPGA as a discriminator. The well-established TRBv3 platform will provide a precise FPGA TDC for a ToT measurement of the discriminated signals. An 8-channel prototype board PaDiWa-AMPS was manufactured and successfully tested in the laboratory and under beam conditions. In this contribution the current status of the read-out concept is shown.

  9. Short-Range Responses of the Kissing Bug Triatoma rubida (Hemiptera: Reduviidae) to Carbon Dioxide, Moisture, and Artificial Light.

    Science.gov (United States)

    Indacochea, Andres; Gard, Charlotte C; Hansen, Immo A; Pierce, Jane; Romero, Alvaro

    2017-08-29

    The hematophagous bug Triatoma rubida is a species of kissing bug that has been marked as a potential vector for the transmission of Chagas disease in the Southern United States and Northern Mexico. However, information on the distribution of T. rubida in these areas is limited. Vector monitoring is crucial to assess disease risk, so effective trapping systems are required. Kissing bugs utilize extrinsic cues to guide host-seeking, aggregation, and dispersal behaviors. These cues have been recognized as high-value targets for exploitation by trapping systems. A modern video-tracking system was used with a four-port olfactometer system to quantitatively assess the behavioral response of T. rubida to cues of known significance. Also, response of T. rubida adults to seven wavelengths of light-emitting diodes (LED) in paired-choice pitfall was evaluated. Behavioral data gathered from these experiments indicate that T. rubida nymphs orient preferentially to airstreams at either 1600 or 3200 ppm carbon dioxide and prefer relative humidity levels of about 30%, while adults are most attracted to 470 nm light. These data may serve to help design an effective trapping system for T. rubida monitoring. Investigations described here also demonstrate the experimental power of combining an olfactometer with a video-tracking system for studying insect behavior.

  10. Mg(2+) Binding Promotes SLV as a Scaffold in Varkud Satellite Ribozyme SLI-SLV Kissing Loop Junction.

    Science.gov (United States)

    Bergonzo, Christina; Cheatham, Thomas E

    2017-07-25

    Though the structure of the substrate stem loop I (SLI)-stem loop V (SLV) kissing loop junction of the Varkud Satellite ribozyme has been experimentally characterized, the dynamics of this Mg(2+)-dependent loop-loop interaction have been elusive. Specifically, each hairpin loop contains a U-turn motif, but only SLV shows a conformational shift triggered by Mg(2+) ion association. Here, we use molecular dynamics simulations to analyze the binding and dynamics of this kissing loop junction. We show that SLV acts as a scaffold, providing stability to the junction. Mg(2+) ions associate with SLV when it is part of the junction in a manner similar to when it is unbound, but there is no specificity in Mg(2+) binding for the SLI loop. This suggests that the entropic penalty of ordering the larger SLI is too high, allowing SLV to act as a scaffold for multiple substrate loop sequences. Copyright © 2017 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  11. 'It's like a personal motivator that you carried around wi' you': utilising self-determination theory to understand men's experiences of using pedometers to increase physical activity in a weight management programme.

    Science.gov (United States)

    Donnachie, Craig; Wyke, Sally; Mutrie, Nanette; Hunt, Kate

    2017-05-05

    Self-monitoring using pedometers is an effective behaviour change technique to support increased physical activity (PA). However, the ways in which pedometers operate as motivational tools in adoption and maintenance of PA is not well understood. This paper investigates men's experiences of pedometers as motivational tools both during and after their participation in a 12-week group-based, weight management programme for overweight/obese men, Football Fans in Training (FFIT). Semi-structured telephone interviews were conducted with 28 men, purposively sampled to include men who did and did not achieve 5% weight loss during the programme. Data were analysed thematically utilising the framework approach, using Self-Determination Theory (SDT) - namely concepts of behavioural regulation and the basic needs of relatedness, competence and autonomy - as an analytical lens. During the programme, FFIT's context and fellow participants supported relatedness and encouraged use of the pedometer. The pedometer was seen to provide tangible proof of progress, thus increasing competence for change, whilst the ability to monitor one's own progress and take remedial action supported autonomy; these men portrayed the pedometer as an 'ally'. However, a minority found the pedometer 'dispiriting' or controlling when it evidenced their inability to meet their PA targets. After the programme, some men no longer used the device as they had fully internalised their motivations for increased PA. In contrast, others continued to use pedometers or progressed to other self-monitoring technologies because it was enjoyable and facilitated maintenance of their increased PA. However, the minority of men who experienced the pedometer as controlling no longer used it. They were less successful in achieving 5% weight loss and appeared reliant on external factors, including support from coach and group members, to maintain motivation. These findings show how self-monitoring using pedometers and

  12. Effectiveness of a universal parental support programme to promote healthy dietary habits and physical activity and to prevent overweight and obesity in 6-year-old children: the Healthy School Start Study, a cluster-randomised controlled trial.

    Science.gov (United States)

    Nyberg, Gisela; Sundblom, Elinor; Norman, Åsa; Bohman, Benjamin; Hagberg, Jan; Elinder, Liselotte Schäfer

    2015-01-01

    To develop and evaluate the effectiveness of a parental support programme to promote healthy dietary and physical activity habits and to prevent overweight and obesity in Swedish children. A cluster-randomised controlled trial was carried out in areas with low to medium socio-economic status. Participants were six-year-old children (n = 243) and their parents. Fourteen pre-school classes were randomly assigned to intervention (n = 7) and control groups (n = 7). The intervention lasted for 6 months and included: 1) Health information for parents, 2) Motivational Interviewing with parents and 3) Teacher-led classroom activities with children. Physical activity was measured by accelerometry, dietary and physical activity habits and parental self-efficacy through a questionnaire. Body weight and height were measured and BMI standard deviation score was calculated. Measurements were conducted at baseline, post-intervention and at 6-months follow-up. Group differences were examined using analysis of covariance and Poisson regression, adjusted for gender and baseline values. There was no significant intervention effect in the primary outcome physical activity. Sub-group analyses showed a significant gender-group interaction in total physical activity (TPA), with girls in the intervention group demonstrating higher TPA during weekends (p = 0.04), as well as in sedentary time, with boys showing more sedentary time in the intervention group (p = 0.03). There was a significantly higher vegetable intake (0.26 servings) in the intervention group compared to the control group (p = 0.003). At follow-up, sub-group analyses showed a sustained effect for boys. The intervention did not affect the prevalence of overweight or obesity. It is possible to influence vegetable intake in children and girls' physical activity through a parental support programme. The programme needs to be intensified in order to increase effectiveness and sustain the effects long-term. These findings are an

  13. Effectiveness of a universal parental support programme to promote healthy dietary habits and physical activity and to prevent overweight and obesity in 6-year-old children: the Healthy School Start Study, a cluster-randomised controlled trial.

    Directory of Open Access Journals (Sweden)

    Gisela Nyberg

    Full Text Available To develop and evaluate the effectiveness of a parental support programme to promote healthy dietary and physical activity habits and to prevent overweight and obesity in Swedish children.A cluster-randomised controlled trial was carried out in areas with low to medium socio-economic status. Participants were six-year-old children (n = 243 and their parents. Fourteen pre-school classes were randomly assigned to intervention (n = 7 and control groups (n = 7. The intervention lasted for 6 months and included: 1 Health information for parents, 2 Motivational Interviewing with parents and 3 Teacher-led classroom activities with children. Physical activity was measured by accelerometry, dietary and physical activity habits and parental self-efficacy through a questionnaire. Body weight and height were measured and BMI standard deviation score was calculated. Measurements were conducted at baseline, post-intervention and at 6-months follow-up. Group differences were examined using analysis of covariance and Poisson regression, adjusted for gender and baseline values.There was no significant intervention effect in the primary outcome physical activity. Sub-group analyses showed a significant gender-group interaction in total physical activity (TPA, with girls in the intervention group demonstrating higher TPA during weekends (p = 0.04, as well as in sedentary time, with boys showing more sedentary time in the intervention group (p = 0.03. There was a significantly higher vegetable intake (0.26 servings in the intervention group compared to the control group (p = 0.003. At follow-up, sub-group analyses showed a sustained effect for boys. The intervention did not affect the prevalence of overweight or obesity.It is possible to influence vegetable intake in children and girls' physical activity through a parental support programme. The programme needs to be intensified in order to increase effectiveness and sustain the effects long-term. These findings

  14. Impact of a school-based intervention on nutritional education and physical activity in primary public schools in Chile (KIND) programme study protocol: cluster randomised controlled trial

    OpenAIRE

    Bustos, Nelly; Olivares, Sonia; Leyton, Bárbara; Cano, Marcelo; Albala,Cecilia

    2016-01-01

    Background Chile has suffered a fast increase in childhood obesity in the last 10 years. As a result, several school programmes have been implemented, however the effectiveness of these needs to be evaluated to identify and prioritize strategies to curve this trend. Methods Cluster randomized controlled trial. Twelve primary public schools chosen at random over three regions of the country will take part in this study. The sample size consisted of a total of 1,655 children. For each region on...

  15. Geometrical consequences of kissing stents and the Covered Endovascular Reconstruction of the Aortic Bifurcation configuration in an in vitro model for endovascular reconstruction of aortic bifurcation

    NARCIS (Netherlands)

    Groot Jebbink, E.; Grimme, F.A.; Goverde, P.C.; Oostayen, J.A.; Slump, C.H.; Reijnen, M.M.P.J.

    2015-01-01

    OBJECTIVE: Kissing stents (KS) are commonly used to treat aortoiliac occlusive disease, but patency results are often lower than those of isolated stents. The Covered Endovascular Reconstruction of the Aortic Bifurcation (CERAB) technique was recently introduced to reconstruct the aortic bifurcation

  16. Geometrical consequences of kissing stents and the covered endovascular reconstruction of the aortic bifurcation configuration in an in vitro model for endovascular reconstruction of aortic bifurcation

    NARCIS (Netherlands)

    Groot Jebbink, Erik; Grimme, Frederike A.B.; Goverde, Peter C.J.M.; Oostayen, van Jacques A.; Slump, Cornelis H.; Reijnen, Michel M.P.J.

    2015-01-01

    Objective: Kissing stents (KS) are commonly used to treat aortoiliac occlusive disease, but patency results are often lower than those of isolated stents. The Covered Endovascular Reconstruction of the Aortic Bifurcation (CERAB) technique was recently introduced to reconstruct the aortic bifurcation

  17. In vivo geometry of the kissing stent and covered endovascular reconstruction of the aortic bifurcation configurations in aortoiliac occlusive disease.

    Science.gov (United States)

    Groot Jebbink, Erik; Ter Mors, Thijs G; Slump, Cornelis H; Geelkerken, Robert H; Holewijn, Suzanne; Reijnen, Michel Mpj

    2017-01-01

    Objectives Various configurations of kissing stent (KS) configurations exist and patency rates vary. In response the covered endovascular reconstruction of the aortic bifurcation configuration was designed to minimize mismatch and improve outcome. The aim of the current study is to compare geometrical mismatch of kissing stent with the covered endovascular reconstruction of the aortic bifurcation configuration in vivo. Methods Post-operative computed tomographic data and patient demographics from 11 covered endovascular reconstruction of the aortic bifurcation and 11 matched kissing stent patients were included. A free hand region of interest and ellipse fitting method were applied to determine mismatch areas and volumes. Conformation of the stents to the vessel wall was expressed using the D-ratio. Results Patients were mostly treated for Rutherford category 2 and 3 (64%) with a lesion classification of TASC C and D in 82%. Radial mismatch area and volume for the covered endovascular reconstruction of the aortic bifurcation group was significantly lower compared to the kissing stent configuration ( P < 0.05). The D-ratio did not significantly differ between groups. Measurements were performed with good intra-class correlation. There were no significant differences in the post-procedural aortoiliac anatomy. Conclusions The present study shows that radial mismatch exists in vivo and that large differences in mismatch exist, in favour of the covered endovascular reconstruction of the aortic bifurcation configuration. Future research should determine if the decreased radial mismatch results in improved local flow profiles and subsequent clinical outcome.

  18. South African antarctic biological research programme

    CSIR Research Space (South Africa)

    SASCAR

    1981-07-01

    Full Text Available This document provides a description of the past, current and planned South African biological research activities in the sub-Antarctic and Antarctic regions. Future activities will fall under one of the five components of the research programme...

  19. Punch Card Programmable Microfluidics

    CERN Document Server

    Korir, George

    2014-01-01

    Small volume fluid handling in single and multiphase microfluidics provides a promising strategy for efficient bio-chemical assays, low-cost point-of-care diagnostics and new approaches to scientific discoveries. However multiple barriers exist towards low-cost field deployment of programmable microfluidics. Incorporating multiple pumps, mixers and discrete valve based control of nanoliter fluids and droplets in an integrated, programmable manner without additional required external components has remained elusive. Combining the idea of punch card programming with arbitrary fluid control, here we describe a self-contained, hand-crank powered, multiplex and robust programmable microfluidic platform. A paper tape encodes information as a series of punched holes. A mechanical reader/actuator reads these paper tapes and correspondingly executes a series of operations onto a microfluidic chip coupled to the platform in a plug-and-play fashion. Enabled by the complexity of codes that can be represented by a series ...

  20. External Mobility Programme

    CERN Document Server

    HR Department

    2007-01-01

    Every year, a significant number of highly-skilled staff members leave the Organization and offer their talents on the European job market. CERN is launching a programme aiming to help staff members to whom the Organization cannot offer an indefinite contract in the transition towards their next employment. The programme, which is based on the establishment of a number of partnerships with potential employers in the private sector, will run on a voluntary basis. Staff members who have received confirmation that they will not be offered an indefinite contract and who are interested in availing themselves of the opportunities offered by the programme, are invited to enrol by following the procedure described at: https://ert.cern.ch/browse_intranet/wd_pds?p_web_page_id=5841 Applications will be processed in the strictest confidence by the Human Resources Department and eligible profiles will then be made available to partner companies for recruitment purposes. Any subsequent ...

  1. The volunteer programme ‘Night Ravens’:

    DEFF Research Database (Denmark)

    Larsen, Britt Østergaard; Bendix Kleif, Helle; Kolodziejczyk, Christophe

    2015-01-01

    The volunteer programme ‘Night Ravens’ (NR) was founded in Sweden in 1987 and has, over the years, developed into a Scandinavian concept covering large areas of Sweden, Denmark, Norway, Greenland and the Faroe Islands. The NR programme is a crime prevention initiative with adults walking the stre......The volunteer programme ‘Night Ravens’ (NR) was founded in Sweden in 1987 and has, over the years, developed into a Scandinavian concept covering large areas of Sweden, Denmark, Norway, Greenland and the Faroe Islands. The NR programme is a crime prevention initiative with adults walking...... the streets at night in identifiable ‘uniforms’ in areas with high activity. The purpose of this study is to evaluate the effects of the NR programme in Denmark based on a volunteer set-up with a less intrusive approach to situational crime prevention than, for instance, hot spot policing. The analyses...... with NR organizations to districts without NR organizations. The results show no difference in the crime rates between Danish postcode districts with and without the NR programme. Hence, we cannot identify positive effects of situational crime prevention when evaluating this Scandinavian volunteer...

  2. Air Quality Monitoring Programme

    DEFF Research Database (Denmark)

    Kemp, K.; Palmgren, F.

    The Danish Air Quality Monitoring Programme (LMP IV) has been revised in accordance with the Framework Directive and the first three daughter directives of SO2, NOx/NO2, PM10, lead, benzene, CO and ozone. PM10 samplers are under installation and the installation will be completed during 2002....... The PM10 results from 2000 are spares, only TSP are thus included in this report. The data sets for year 2000 is complete for many stations. The monitoring programme consists of 10 stations plus 2 extra stations under the Municipality of Copenhagen. The SO2 and lead levels are still decreasing and far...

  3. A programmer's geometry

    CERN Document Server

    Bowyer, Adrian

    1983-01-01

    A Programmer's Geometry provides a guide in programming geometric shapes. The book presents formulas and examples of computer representation and coding of geometry. Each of the nine chapters of the text deals with the representation and solution of a specific geometrical problem, such as areas, vectors, and volumes. The last chapter provides a brief discussion on generating image through a computer. The codes presented in the book are written in FORTRAN 77. The text will be of great use to programmers who are working on projects that involve geometric calculations.

  4. Computer mathematics for programmers

    CERN Document Server

    Abney, Darrell H; Sibrel, Donald W

    1985-01-01

    Computer Mathematics for Programmers presents the Mathematics that is essential to the computer programmer.The book is comprised of 10 chapters. The first chapter introduces several computer number systems. Chapter 2 shows how to perform arithmetic operations using the number systems introduced in Chapter 1. The third chapter covers the way numbers are stored in computers, how the computer performs arithmetic on real numbers and integers, and how round-off errors are generated in computer programs. Chapter 4 details the use of algorithms and flowcharting as problem-solving tools for computer p

  5. Coronary bifurcation lesions treated with simple approach (from the Cordoba & Las Palmas [CORPAL] Kiss Trial).

    Science.gov (United States)

    Pan, Manuel; Medina, Alfonso; Suárez de Lezo, José; Romero, Miguel; Segura, Jose; Martín, Pedro; Suárez de Lezo, Javier; Hernández, Enrique; Mazuelos, Francisco; Moreno, Alvaro; Pavlovic, Djordje; Ojeda, Soledad; Toledano, Francisco; Leon, Carmen

    2011-05-15

    The present report compared the incidence of 1-year clinical events in patients with bifurcation lesions that had been treated with a simple approach who were randomized to either a simultaneous final kissing balloon (KB) or an isolated side-branch (SB) balloon post-dilation. From February 2007 to December 2008, 293 patients with all types of Medina bifurcation lesions were enrolled in a prospective study. All patients underwent implantation of a sirolimus- or everolimus-eluting stent across the bifurcation and provisional SB stenting. Patients with no SB ostial compromise or those needing a second stent were excluded from the present study (n = 49). The eligible patients were randomly assigned to treatment with final KB inflation (n = 124, KB group) or isolated balloon after dilation (n = 120, non-KB group). No significant differences were found between the patients from the KB and non-KB groups in terms of age, risk factors, clinical status, or location of the bifurcation lesions. The angiographic data and immediate results were also similar in both groups. Four patients experienced a non-Q-wave acute myocardial infarction in the hospital: three (2%) from the KB group and one (1%) from the non-KB group. Two in-hospital deaths occurred in the non-KB group. Target lesion revascularization was required in 7 patients (3%): 5 from the KB group and 2 from the non-KB group. Late mortality occurred in 3 patients from the KB group and 2 patients from the non-KB group. The incidence of major events at 1 year (death, target lesion revascularization, or acute myocardial infarction) was similar in both groups: 11 (9%) from the KB group and 7 (6%) from the non-KB group (p = NS). In conclusion, no differences in the clinical outcome at 1 year of follow-up were observed between the patients with bifurcation lesions treated with a simple approach and either a simultaneous final KB or an isolated SB balloon post-dilation. Copyright © 2011 Elsevier Inc. All rights reserved.

  6. The differential expression of Kiss1, MMP9 and angiogenic regulators across the feto-maternal interface of healthy human pregnancies: implications for trophoblast invasion and vessel development.

    Science.gov (United States)

    Matjila, Mushi; Millar, Robert; van der Spuy, Zephne; Katz, Arieh

    2013-01-01

    Genes involved in invasion of trophoblast cells and angiogenesis are crucial in determining pregnancy outcome. We therefore studied expression profiles of these genes in both fetal and maternal tissues to enhance our understanding of feto-maternal dialogue. We investigated the expression of genes involved in trophoblast invasion, namely Kiss1, Kiss1 Receptor (Kiss1R) and MMP9 as well as the expression of angiogenic ligands Vascular Endothelial Growth Factor-A (VEGF-A) and Prokineticin-1 (PROK1) and their respective receptors (VEGFR1, VEGFR2 and PROK1R) across the feto-maternal interface of healthy human pregnancies. The placenta, placental bed and decidua parietalis were sampled at elective caesarean delivery. Real-time RT-PCR was used to investigate transcription, while immunohistochemistry and western blot analyses were utilized to study protein expression. We found that the expression of Kiss1 (p<0.001), Kiss1R (p<0.05) and MMP9 (p<0.01) were higher in the placenta compared to the placental bed and decidua parietalis. In contrast, the expression of VEGF-A was highest in the placental bed (p<0.001). While VEGFR1 expression was highest in the placenta (p<0.01), the expression of VEGFR2 was highest in the placental bed (p<0.001). Lastly, both PROK1 (p<0.001) and its receptor PROK1R (p<0.001) had highest expression in the placenta. Genes associated with trophoblast invasion were highly expressed in the placenta which could suggest that the influence on invasion capacity may largely be exercised at the fetal level. Furthermore, our findings on angiogenic gene expression profiles suggest that angiogenesis may be regulated by two distinct pathways with the PROK1/PROK1R system specifically mediating angiogenesis in the fetus and VEGFA/VEGFR2 ligand-receptor pair predominantly mediating maternal angiogenesis.

  7. Photoperiod regulates the differential expression of KiSS-1 and GPR54 in various tissues and sexes of striped hamster.

    Science.gov (United States)

    Li, S N; Xue, H L; Zhang, Q; Xu, J H; Wang, S; Chen, L; Xu, L X

    2015-10-30

    In order to investigate how photoperiod affects the expression of KiSS-1/G-protein-coupled receptor 54 (GPR54), the hypothalamus, the ovaries and testicles of the striped hamster were studied following photoperiod treatment and melatonin injection. We found that the melatonin concentration in the blood of the striped hamster was consistent with the treated photoperiods and that the photoperiod had significant effects on KiSS-1 expression, but the effect of photoperiod on GPR54 expression differed among tissues and sexes. The relative expression of KiSS-1 in the gonads of the male and female striped hamster was significantly higher than that in the hypothalamus, while the difference in GPR54 between the gonads and hypothalamus was inconsistent between the male and female striped hamster. In the striped hamster injected with melatonin, the expression level of KiSS-1 in the gonads and hypothalamus of the male and female striped hamster was significantly decreased; the expression level of GnRH in the gonads was also significantly decreased; the expression of GPR54 in the hypothalamus was increased. In the hypothalamus, a significant positive correlation existed between KiSS-1 and GnRH expression, while a negative correlation was observed between GPR54 and GnRH expression. These results suggest that the photoperiod-melatonin-KiSS-1-GnRH pathway plays an important role in the seasonal reproduction mechanism of the striped hamster. Additionally, photoperiod or melatonin may not be a direct regulatory factor of GPR54 expression.

  8. A programmable molecular robot.

    Science.gov (United States)

    Muscat, Richard A; Bath, Jonathan; Turberfield, Andrew J

    2011-03-09

    We have developed a programmable and auton-omous molecular robot whose motion is fueled by DNA hybridization. Instructions determining the path to be followed are programmed into the fuel molecules, allowing precise control of cargo motion on a branched track.

  9. User programmable virtualized networks

    NARCIS (Netherlands)

    Meijer, R.J.; Strijkers, R.J.; Gommans, L.; Laat, C.de

    2006-01-01

    This paper introduces the concept of a User Programmable Virtualized Network, which allows networks to deliver application specific services using network element components that developers can program as part of a users application. The use of special tokens in data or control packets is the basis

  10. Progressive Retirement Programme

    CERN Multimedia

    HR Department

    2009-01-01

    Following the Standing Concertation Committee meeting of 2 December 2008, please note that the Progressive Retirement Programme has been extended by one year, i.e. until 31 March 2010. Further information is available on : https://hr-services.web.cern.ch/hr-services/services-Ben/prp/prp.asp HR Department, tel. 73903

  11. The European Programme Manager

    DEFF Research Database (Denmark)

    Larson, Anne; Bergman, E.; Ehlers, S.;

    The publication is a result of a cooperation between organisations in six European countries with the aim to develop a common European education for programme managers. It contains of a description of the different elements of the education together with a number of case-studies from the counties...

  12. Air Quality Monitoring Programme

    DEFF Research Database (Denmark)

    Kemp, K.; Palmgren, F.

    The Danish Air Quality Monitoring Programme (LMP IV) has been revised in accordance with the Framework Directive and the first three daughter directives of SO2, NOx/NO2, PM10, lead, benzene, CO and ozone. PM10 samplers are under installation and the installation will be completed during 2002...

  13. VAM programme 14

    Energy Technology Data Exchange (ETDEWEB)

    Youngman, Michael [National Radiological Protection Board, Chilton (United Kingdom)

    1995-05-01

    Since 1988, the Department of Trade and Industry (DTI) has supported a Valid Analytical Measurement (VAM) initiative. VAM programming 14 is concerned with sampling and measurement of aerosols and particulates in the gas phase. The programme of work is summarised here. (author).

  14. En model for programmer

    DEFF Research Database (Denmark)

    Christensen, Henrik Bærbak

    Dette undervisningsmateriale beskriver en model for, hvordan programmer er opbygget. Materialet er skrevet til brug i det gymnasiale forsøgsfag Informationsteknologi. Seneste version af dette undervisningsmateriale kan findes på http:// www.imhotep.dk. Tak til Elisabeth Husum for en kritisk...

  15. A 10-year interval study to compare the prevalence and risk factors of female sexual dysfunction in Korea: the Korean internet sexuality survey (KISS) 2014.

    Science.gov (United States)

    Shin, H; Min, B; Park, J; Son, H

    2016-12-08

    Female sexual dysfunction (FSD) is defined as disturbances in sexual desire and the psychophysiological changes that characterize the sexual response cycle, which cause marked distress and interpersonal difficulty. Female sexual dysfunction is strongly related to socio-cultural characteristics of a certain region and can change over time. Authors previously reported the features of FSD in Korea through an analysis of an internet-based survey conducted in 2004. This time, we designed a 10-year interval study to compare the prevalence of and risk factors of FSD through the Korean Internet Sexuality Survey (KISS) 2014. The internet-based survey was targeted towards 20-59-year-old women, who were asked to answer the questionnaire only if they were sexually active. In total, 516 responses were analyzed and the mean age of the subjects was 38.1±10.3 years. We defined FSD as having a total FSFI score of under 25.0, and resulted in a prevalence of FSD of 46.7%. The prevalence of FSD in each age group increased with age from 23.7, 45.4, 51.4 to 72%, for those in their 20, 30, 40 and 50s, respectively. Among the 50s age group, lubrication and pain domain dysfunctions were highly prevalent. The risk factors for FSD were identified as constipation, depression, incontinence, age of first intercourse, number of sexual partners and the use of oral contraceptive pills. Although the some demographic features of this study was different with those of the 2004 repot, the prevalence of FSD was similar with that reported previously in 2004 and a few risk factors of FSD was consistently significant in these interval studies.International Journal of Impotence Research advance online publication, 8 December 2016; doi:10.1038/ijir.2016.41.

  16. EdAl-2 (Educació en Alimentació) programme: reproducibility of a cluster randomised, interventional, primary-school-based study to induce healthier lifestyle activities in children

    OpenAIRE

    2014-01-01

    Objectives To assess the reproducibility of an educational intervention EdAl-2 ( Educació en Alimentació) programme in ‘Terres de l'Ebre’ (Spain), over 22 months, to improve lifestyles, including diet and physical activity (PA). Design Reproduction of a cluster randomised controlled trial. Setting Two semi-rural town-group primary-school clusters were randomly assigned to the intervention or control group. Participants Pupils (n=690) of whom 320 constituted the intervention group (1 cluster) ...

  17. Evaluating the effectiveness of a schools-based programme to promote exercise self-efficacy in children and young people with risk factors for obesity: Steps to active kids (STAK

    Directory of Open Access Journals (Sweden)

    Yang Min

    2011-10-01

    Full Text Available Abstract Background Low levels of physical activity in children have been linked to an increased risk of obesity, but many children lack confidence in relation to exercise (exercise self-efficacy. Factors which can impact on confidence include a chronic health condition such as asthma, poor motor skills and being overweight. Increasing levels of physical activity have obvious benefits for children with asthma and children who are overweight, but few activity interventions with children specifically target children with low exercise self-efficacy (ESE. This study aims to evaluate the efficacy and feasibility of a schools-based activity programme suitable for children with risk factors for adult obesity, including asthma, overweight and low exercise self-efficacy. Methods/Design A clustered (at the level of school RCT will be used to compare a targeted, 10 week, stepped activity programme (activity diary, dance DVD, circuit-training and motivational interviewing designed to promote ESE. We will recruit 20 primary schools to participate in the intervention and 9-11 year old children will be screened for low levels of ESE, asthma and overweight. In order to provide sufficient power to detect a difference in primary outcomes (Body Mass Index-BMI & ESE at 12 month follow-up between children in the intervention schools and control schools, the target sample size is 396. Assessments of BMI, ESE, waist circumference, peak flow, activity levels and emotional and behavioural difficulties will be made at baseline, 4 months and 12 month follow-up. Discussion We aim to increase ESE and levels of physical activity in children with risk factors for adult obesity. The outcomes of this study will inform policy makers about the feasibility, acceptability and effectiveness of delivering targeted health interventions within a school setting. Trial Registration ISRCTN Register no. ISRCTN12650001

  18. Evaluating the effectiveness of a schools-based programme to promote exercise self-efficacy in children and young people with risk factors for obesity: Steps to active kids (STAK)

    Science.gov (United States)

    2011-01-01

    Background Low levels of physical activity in children have been linked to an increased risk of obesity, but many children lack confidence in relation to exercise (exercise self-efficacy). Factors which can impact on confidence include a chronic health condition such as asthma, poor motor skills and being overweight. Increasing levels of physical activity have obvious benefits for children with asthma and children who are overweight, but few activity interventions with children specifically target children with low exercise self-efficacy (ESE). This study aims to evaluate the efficacy and feasibility of a schools-based activity programme suitable for children with risk factors for adult obesity, including asthma, overweight and low exercise self-efficacy. Methods/Design A clustered (at the level of school) RCT will be used to compare a targeted, 10 week, stepped activity programme (activity diary, dance DVD, circuit-training and motivational interviewing) designed to promote ESE. We will recruit 20 primary schools to participate in the intervention and 9-11 year old children will be screened for low levels of ESE, asthma and overweight. In order to provide sufficient power to detect a difference in primary outcomes (Body Mass Index-BMI & ESE at 12 month follow-up) between children in the intervention schools and control schools, the target sample size is 396. Assessments of BMI, ESE, waist circumference, peak flow, activity levels and emotional and behavioural difficulties will be made at baseline, 4 months and 12 month follow-up. Discussion We aim to increase ESE and levels of physical activity in children with risk factors for adult obesity. The outcomes of this study will inform policy makers about the feasibility, acceptability and effectiveness of delivering targeted health interventions within a school setting. Trial Registration ISRCTN Register no. ISRCTN12650001 PMID:22029547

  19. Evaluating the effectiveness of a schools-based programme to promote exercise self-efficacy in children and young people with risk factors for obesity: steps to active kids (STAK).

    Science.gov (United States)

    Glazebrook, Cris; Batty, Martin J; Mullan, Nivette; Macdonald, Ian; Nathan, Dilip; Sayal, Kapil; Smyth, Alan; Yang, Min; Guo, Boliang; Hollis, Chris

    2011-10-26

    Low levels of physical activity in children have been linked to an increased risk of obesity, but many children lack confidence in relation to exercise (exercise self-efficacy). Factors which can impact on confidence include a chronic health condition such as asthma, poor motor skills and being overweight. Increasing levels of physical activity have obvious benefits for children with asthma and children who are overweight, but few activity interventions with children specifically target children with low exercise self-efficacy (ESE). This study aims to evaluate the efficacy and feasibility of a schools-based activity programme suitable for children with risk factors for adult obesity, including asthma, overweight and low exercise self-efficacy. A clustered (at the level of school) RCT will be used to compare a targeted, 10 week, stepped activity programme (activity diary, dance DVD, circuit-training and motivational interviewing) designed to promote ESE. We will recruit 20 primary schools to participate in the intervention and 9-11 year old children will be screened for low levels of ESE, asthma and overweight. In order to provide sufficient power to detect a difference in primary outcomes (Body Mass Index-BMI & ESE at 12 month follow-up) between children in the intervention schools and control schools, the target sample size is 396. Assessments of BMI, ESE, waist circumference, peak flow, activity levels and emotional and behavioural difficulties will be made at baseline, 4 months and 12 month follow-up. We aim to increase ESE and levels of physical activity in children with risk factors for adult obesity. The outcomes of this study will inform policy makers about the feasibility, acceptability and effectiveness of delivering targeted health interventions within a school setting. ISRCTN Register no. ISRCTN12650001.

  20. Framework Programmable Platform for the Advanced Software Development Workstation (FPP/ASDW). Demonstration framework document. Volume 1: Concepts and activity descriptions

    Science.gov (United States)

    Mayer, Richard J.; Blinn, Thomas M.; Dewitte, Paul S.; Crump, John W.; Ackley, Keith A.

    1992-01-01

    The Framework Programmable Software Development Platform (FPP) is a project aimed at effectively combining tool and data integration mechanisms with a model of the software development process to provide an intelligent integrated software development environment. Guided by the model, this system development framework will take advantage of an integrated operating environment to automate effectively the management of the software development process so that costly mistakes during the development phase can be eliminated. The Advanced Software Development Workstation (ASDW) program is conducting research into development of advanced technologies for Computer Aided Software Engineering (CASE).

  1. Coronary bifurcation lesions treated with double kissing crush technique compared to classical crush technique: serial intravascular ultrasound analysis

    Institute of Scientific and Technical Information of China (English)

    SHAN Shou-jie; YE Fei; LIU Zhi-zhong; TIAN Nai-liang; ZHANG Jun-jie; HEN Shao-liang

    2013-01-01

    Background The double kissing (DK) crush technique is a modified version of the crush technique.It is specifically designed to increase the success rate of the final kissing balloon post-dilatation,but its efficacy and safety remain unclear.Methods Data were obtained from the DKCRUSH-I trial,a prospective,randomized,multi-center study to evaluate safety and efficacy.Post-procedural and eight-month follow-up intravascular ultrasound (IVUS) analysis was available in 61 cases.Volumetric analysis using Simpson's method within the Taxus stent,and cross-sectional analysis at the five sites of the main vessel (MV) and three sites of the side branch (SB) were performed.Impact of the bifurcation angle on stent expansion at the carina was also evaluated.Results Stent expansion in the SB ostium was significantly less in the classical crush group ((53.81±13.51)%) than in the DK crush group ((72.27±11.46)%) (P=-0.04).For the MV,the incidence of incomplete crush was 41.9% in the DK group and 70.0% in the classical group (P=-0.03).The percentage of neointimal area at the ostium had a tendency to be smaller in the DK group compared with the classical group ((16.4±19.2)% vs.(22.8±27.1)%,P=-0.06).The optimal threshold of post-procedural minimum stent area (MSA) to predict follow-up minimum lumen area (MLA) <4.0 mm2 at the SB ostium was 4.55 mm2,yielding an area under the curve of 0.80 (95% confidence interval:0.61 to 0.92).Conclusion Our data suggest that the DK crush technique is associated with improved quality of the final kissing balloon inflation (FKBI) and had smaller optimal cutoff value of post-procedural MSA at the SB ostium.

  2. Observation of a Change in Twist of an RNA Kissing Complex Using the Angular Dependence of Fluorescence Resonance Energy Transfer

    Science.gov (United States)

    Rahmanseresht, Sheema; Milas, Peker; Parrot, Louis; Gamari, Ben D.; Goldner, Lori S.

    2015-01-01

    We report on the observation of a change in the bend angle of an RNA kissing complex upon Rop binding using single-molecular-pair FRET. The angular relationship between the dyes, rather than the distance between them, is shown to be responsible for the observed change in energy transfer. It has long been thought that Rop increases the bend angle of the R1inv-R2inv complex upon binding, but this has never been directly observed. In contrast, we find an increase in FRET upon the addition of Rop that is shown via modeling to be consistent with a decrease in the bend angle of the complex of $-15^{\\circ}\\pm7^{\\circ}$. The model predicts FRET from dye trajectories generated using molecular dynamics simulations of Cy3 and Cy5 attached to $5'$ terminal cytosine or guanosine on RNA. While FRET is commonly used to observe global changes in molecular structure attributed to changes in the distance between dyes, it is rarely, if ever, used to elucidate angular changes. Subtle global changes in molecular structure upon binding are generally difficult to discern using NMR or crystallography, but FRET is well suited to observe these changes because of its sensitivity at interdye distance around the F\\"orster radius, $R_F\\approx5$ nm. While FRET is often referred to and used as a "molecular ruler" for distances around $R_F$, for dye pairs that have minimal rotational freedom, FRET can also be used to observe changes in structure for which there is no significant change in distance between the dyes. This is the case for the R1inv-R2inv kissing complex studied here. This complex is derived from the RNA I - RNA II complex in E. coli. RNA II is a primer for replication of the ColE1 plasmid, its function is modulated by interaction with RNA II. Rop is known to stabilize the complex, and it is also known to bind kissing complexes in a structure, but not sequence, dependent fashion.

  3. The 'Walking for Wellbeing in the West' randomised controlled trial of a pedometer-based walking programme in combination with physical activity consultation with 12 month follow-up: rationale and study design

    Directory of Open Access Journals (Sweden)

    Ogilvie David

    2008-07-01

    Full Text Available Abstract Background Scotland has a policy aimed at increasing physical activity levels in the population, but evidence on how to achieve this is still developing. Studies that focus on encouraging real world participants to start physical activity in their settings are needed. The Walking for Well-being in the West study was designed to assess the effectiveness of a pedometer-based walking programme in combination with physical activity consultation. The study was multi-disciplinary and based in the community. Walking for Well-being in the West investigated whether Scottish men and women, who were not achieving the current physical activity recommendation, increased and maintained walking behaviour over a 12 month period. This paper outlines the rationale and design of this innovative and pragmatic study. Methods Participants were randomised into two groups: Group 1: Intervention (pedometer-based walking programme combined with a series of physical activity consultations; Group 2: Waiting list control for 12 weeks (followed by minimal pedometer-based intervention. Physical activity (primary outcome was measured using pedometer step counts (7 day and the International Physical Activity Questionnaire (long version. Psychological processes were measured using questionnaires relating to the Transtheoretical Model of Behaviour Change, mood (Positive and Negative Affect Schedule and quality of life (Euroqol EQ-5D instrument. Physiological measures included anthropometric and metabolic outcomes. Environmental influences were assessed subjectively (Neighbourhood Quality of Life Survey and objectively (neighbourhood audit tool and GIS mapping. The qualitative evaluation employed observation, semi-structured interviews and focus groups. A supplementary study undertook an economic evaluation. Discussion Data analysis is on-going. Walking for Well-being in the West will demonstrate if a pedometer based walking programme, in combination with physical

  4. The Possibility of Social Change and Pop Music in Serbia: Šobaja’s Three Kisses – A Case Study

    Directory of Open Access Journals (Sweden)

    Aleksandar Brkić

    2016-02-01

    Full Text Available Can social change be brought about only in a radical manner? Is a radical manner of bringing about change possible at all? When we say social change, do we mean "revolution"? Is there a different way of looking at social change, and what is the role of elite culture and that of popular culture in these processes? Has the temporally and contextually limited effect of change and guerilla subversion through popular culture proved more effective? This small-scale study aims to raise the question of whether pop music can contribute to positive changes in society, and examines the conditions for this possibility through the case study of Nebojša "Šobaja" Anđelković’s song Three Kisses, declared the biggest music hit of 2009 in Serbia.

  5. The INTEGRAL Core Observing Programme

    DEFF Research Database (Denmark)

    Winkler, C.; Gehrels, N.; Lund, Niels

    1999-01-01

    The Core Programme of the INTEGRAL mission is defined as the portion of the scientific programme covering the guaranteed time observations for the INTEGRAL Science Working Team. This paper describes the current status of the Core Programme preparations and summarizes the key elements...

  6. The INTEGRAL Core Observing Programme

    DEFF Research Database (Denmark)

    Winkler, C.; Gehrels, N.; Lund, Niels

    1999-01-01

    The Core Programme of the INTEGRAL mission is defined as the portion of the scientific programme covering the guaranteed time observations for the INTEGRAL Science Working Team. This paper describes the current status of the Core Programme preparations and summarizes the key elements...

  7. Dynamic Regulation of Hypothalamic DMXL2, KISS1, and RFRP Expression During Postnatal Development in Non-Human Primates.

    Science.gov (United States)

    Wahab, Fazal; Drummer, Charis; Schlatt, Stefan; Behr, Rüdiger

    2016-12-12

    The neurobiological mechanism of puberty onset in primates is currently only partly understood. A recent study reported an important role of Dmx-like 2 (DMXL2), a gene encoding rabconnectin-3α vesicular protein, in human subjects with mental retardation and neuroendocrine impairment of reproduction. To further characterize the potential role of DMXL2 in the regulation of reproduction, we analyzed the expression of DMXL2 in hypothalami of newborn, infantile, juvenile, pubertal, and postpubertal female and male common marmoset monkeys. Additionally, as the relative hypothalamic levels of gonadotropin-inhibitory hormone (GnIH) transcript during postnatal development are unknown in primates, we also quantified messenger RNA (mRNA) levels of RFRP, a gene encoding GnIH. Moreover, the transcript levels of kisspeptin, a well-known regulator of the hypothalamic neurohormonal axis controlling reproduction, were also checked. Transcript and protein levels of DMXL2 and Kiss1 transcript levels increase from the newborn to the infantile and from the juvenile (prepubertal) to the pubertal and the postpubertal period. We also noted a clear upsurge in RFRP transcript levels in the prepubertal period. In conclusion, the hypothalamic expressions of Kiss1 and DMXL2 mRNA increase during infantile, pubertal, and adult stages compared to newborn and juvenile stages in common marmoset monkeys. In contrast, the expression of RFRP mRNA upsurges in juvenile monkeys. Further mechanistic studies are needed to characterize the potential inhibitory role of the GnIH-GPR147 signaling in the prepubertal period and the role of DMXL2 in the molecular cascade regulating the neuroendocrine reproductive axis in primates.

  8. Economic viability of new launched school lunch programmes

    DEFF Research Database (Denmark)

    Jensen, Jørgen Dejgård; Smed, Sinne; Mørkbak, Morten Raun

    2013-01-01

    activities related to the schools’ support and the users’ feeling of ownership, as well as internal professionalism and leadership in the implementation of the school lunch programme are important for the viability of the programme. Strong performance on the latter factors might to some extent compensate...

  9. The VIDA Programme

    DEFF Research Database (Denmark)

    Jensen, Bente; Iannone, Rosa Lisa

    and Innovation’ within the project ‘Curriculum Quality Analysis and Impact Review of European Education and Care’ (CARE). The programme at the centre of this case builds on theory drawn from research on child development, social disadvantage related to issues of social inequality, and research on organisational...... learning and innovation. The latter field has tended to focus primarily on technological innovation, leaving socially-driven innovation in the shade (Dawson & Daniel, 2010). Here, in the field of early childhood education and care (ECEC), we draw on Dawson and Daniel’s (ibid., p. 10) definition...... programme period (2010-2013) and beyond?; 2) What is the impact of the VIDA approach to professional development on i) educators’ practices regarding high quality ECEC (output), ii) child outcomes (outcome), and iii) improved practice at the municipal level (impact in a broader sense)?; and 3) Which factors...

  10. SUMMER STUDENT LECTURE PROGRAMME

    CERN Multimedia

    Academic Training; Tel. 73127

    2001-01-01

    Main Auditorium bldg. 500 Date Time Lecturer Title Monday 13 August 9:15 10:15 11:15 Student Session (3/3) Course Review Course Review Tuesday 14 August 16:00 Poster Session Further information can be obtained on the web at the following URL: Summer Student Lecture ProgrammeSummer Student Lectures are available at: http://agenda.cern.ch/SSLP2001

  11. NSF announces diversity programme

    Science.gov (United States)

    Kruesi, Liz

    2016-04-01

    The US National Science Foundation (NSF) has initiated a new funding programme that will create schemes to increase diversity in science, technology, engineering and mathematics (STEM). The initiative - Inclusion across the Nation of Communities of Learners of Underrepresented Discoverers in Engineering and Science (INCLUDES) - aims to increase the participation of women, those with a low socioeconomic status, people with disabilities and those from minority racial backgrounds.

  12. Programmable mechanical metamaterials.

    Science.gov (United States)

    Florijn, Bastiaan; Coulais, Corentin; van Hecke, Martin

    2014-10-24

    We create mechanical metamaterials whose response to uniaxial compression can be programmed by lateral confinement, allowing monotonic, nonmonotonic, and hysteretic behavior. These functionalities arise from a broken rotational symmetry which causes highly nonlinear coupling of deformations along the two primary axes of these metamaterials. We introduce a soft mechanism model which captures the programmable mechanics, and outline a general design strategy for confined mechanical metamaterials. Finally, we show how inhomogeneous confinement can be explored to create multistability and giant hysteresis.

  13. Punch card programmable microfluidics.

    Science.gov (United States)

    Korir, George; Prakash, Manu

    2015-01-01

    Small volume fluid handling in single and multiphase microfluidics provides a promising strategy for efficient bio-chemical assays, low-cost point-of-care diagnostics and new approaches to scientific discoveries. However multiple barriers exist towards low-cost field deployment of programmable microfluidics. Incorporating multiple pumps, mixers and discrete valve based control of nanoliter fluids and droplets in an integrated, programmable manner without additional required external components has remained elusive. Combining the idea of punch card programming with arbitrary fluid control, here we describe a self-contained, hand-crank powered, multiplex and robust programmable microfluidic platform. A paper tape encodes information as a series of punched holes. A mechanical reader/actuator reads these paper tapes and correspondingly executes operations onto a microfluidic chip coupled to the platform in a plug-and-play fashion. Enabled by the complexity of codes that can be represented by a series of holes in punched paper tapes, we demonstrate independent control of 15 on-chip pumps with enhanced mixing, normally-closed valves and a novel on-demand impact-based droplet generator. We demonstrate robustness of operation by encoding a string of characters representing the word "PUNCHCARD MICROFLUIDICS" using the droplet generator. Multiplexing is demonstrated by implementing an example colorimetric water quality assays for pH, ammonia, nitrite and nitrate content in different water samples. With its portable and robust design, low cost and ease-of-use, we envision punch card programmable microfluidics will bring complex control of microfluidic chips into field-based applications in low-resource settings and in the hands of children around the world.

  14. Punch card programmable microfluidics.

    Directory of Open Access Journals (Sweden)

    George Korir

    Full Text Available Small volume fluid handling in single and multiphase microfluidics provides a promising strategy for efficient bio-chemical assays, low-cost point-of-care diagnostics and new approaches to scientific discoveries. However multiple barriers exist towards low-cost field deployment of programmable microfluidics. Incorporating multiple pumps, mixers and discrete valve based control of nanoliter fluids and droplets in an integrated, programmable manner without additional required external components has remained elusive. Combining the idea of punch card programming with arbitrary fluid control, here we describe a self-contained, hand-crank powered, multiplex and robust programmable microfluidic platform. A paper tape encodes information as a series of punched holes. A mechanical reader/actuator reads these paper tapes and correspondingly executes operations onto a microfluidic chip coupled to the platform in a plug-and-play fashion. Enabled by the complexity of codes that can be represented by a series of holes in punched paper tapes, we demonstrate independent control of 15 on-chip pumps with enhanced mixing, normally-closed valves and a novel on-demand impact-based droplet generator. We demonstrate robustness of operation by encoding a string of characters representing the word "PUNCHCARD MICROFLUIDICS" using the droplet generator. Multiplexing is demonstrated by implementing an example colorimetric water quality assays for pH, ammonia, nitrite and nitrate content in different water samples. With its portable and robust design, low cost and ease-of-use, we envision punch card programmable microfluidics will bring complex control of microfluidic chips into field-based applications in low-resource settings and in the hands of children around the world.

  15. The Mathematica programmer

    CERN Document Server

    Maeder, Roman E

    1994-01-01

    The Mathematica Programmer covers the fundamental programming paradigms and applications of programming languages. This book is organized into two parts encompassing 10 chapters. Part 1 begins with an overview of the programming paradigms. This part also treats abstract data types, polymorphism and message passing, object-oriented programming, and relational databases. Part 2 looks into the practical aspects of programming languages, including in lists and power series, fractal curves, and minimal surfaces.This book will prove useful to mathematicians and computer scientists.

  16. Programmable Quantitative DNA Nanothermometers.

    Science.gov (United States)

    Gareau, David; Desrosiers, Arnaud; Vallée-Bélisle, Alexis

    2016-07-13

    Developing molecules, switches, probes or nanomaterials that are able to respond to specific temperature changes should prove of utility for several applications in nanotechnology. Here, we describe bioinspired strategies to design DNA thermoswitches with programmable linear response ranges that can provide either a precise ultrasensitive response over a desired, small temperature interval (±0.05 °C) or an extended linear response over a wide temperature range (e.g., from 25 to 90 °C). Using structural modifications or inexpensive DNA stabilizers, we show that we can tune the transition midpoints of DNA thermometers from 30 to 85 °C. Using multimeric switch architectures, we are able to create ultrasensitive thermometers that display large quantitative fluorescence gains within small temperature variation (e.g., > 700% over 10 °C). Using a combination of thermoswitches of different stabilities or a mix of stabilizers of various strengths, we can create extended thermometers that respond linearly up to 50 °C in temperature range. Here, we demonstrate the reversibility, robustness, and efficiency of these programmable DNA thermometers by monitoring temperature change inside individual wells during polymerase chain reactions. We discuss the potential applications of these programmable DNA thermoswitches in various nanotechnology fields including cell imaging, nanofluidics, nanomedecine, nanoelectronics, nanomaterial, and synthetic biology.

  17. A new video programme

    CERN Multimedia

    CERN video productions

    2011-01-01

    "What's new @ CERN?", a new monthly video programme, will be broadcast on the Monday of every month on webcast.cern.ch. Aimed at the general public, the programme will cover the latest CERN news, with guests and explanatory features. Tune in on Monday 3 October at 4 pm (CET) to see the programme in English, and then at 4:20 pm (CET) for the French version.   var flash_video_player=get_video_player_path(); insert_player_for_external('Video/Public/Movies/2011/CERN-MOVIE-2011-129/CERN-MOVIE-2011-129-0753-kbps-640x360-25-fps-audio-64-kbps-44-kHz-stereo', 'mms://mediastream.cern.ch/MediaArchive/Video/Public/Movies/2011/CERN-MOVIE-2011-129/CERN-MOVIE-2011-129-Multirate-200-to-753-kbps-640x360-25-fps.wmv', 'false', 480, 360, 'https://mediastream.cern.ch/MediaArchive/Video/Public/Movies/2011/CERN-MOVIE-2011-129/CERN-MOVIE-2011-129-posterframe-640x360-at-10-percent.jpg', '1383406', true, 'Video/Public/Movies/2011/CERN-MOVIE-2011-129/CERN-MOVIE-2011-129-0600-kbps-maxH-360-25-fps-...

  18. 2001 - 2002 ACADEMIC TRAINING PROGRAMME

    CERN Multimedia

    Academic Training; Tel. 73127

    2001-01-01

    1ST TERM 1ST OCTOBER - 23 NOVEMBER 2001 LECTURE SERIES FOR POSTGRADUATE STUDENTS The Autumn term of the Academic Training Programme is about to start. As usual, the first term includes lectures primarily dedicated to Post-graduate students. These are meant to help students complement the courses available from their home Universities with lectures on topics close to CERN activities. The lectures are nevertheless open to all CERN staff, and in particular to young Fellows. This year's series include courses on Accelerator Physics, on Field Theory, and on Symmetry Breaking Phenomena in Physics. The course on Accelerators by Dr. Wilson has been a regular feature on the Academic Training programme for many previous editions. This year, the course will be updated to include new sections on Colliders and on future facilities such as the Neutrino Factory. A good introduction to this very successful course can be found in the previous version of these lectures, available from the Web Lecture Archive Project: http://w...

  19. Sellafield Decommissioning Programme - Update and Lessons Learned

    Energy Technology Data Exchange (ETDEWEB)

    Lutwyche, P. R.; Challinor, S. F.

    2003-02-24

    The Sellafield site in North West England has over 240 active facilities covering the full nuclear cycle from fuel manufacture through generation, reprocessing and waste treatment. The Sellafield decommissioning programme was formally initiated in the mid 1980s though several plants had been decommissioned prior to this primarily to create space for other plants. Since the initiation of the programme 7 plants have been completely decommissioned, significant progress has been made in a further 16 and a total of 56 major project phases have been completed. This programme update will explain the decommissioning arrangements and strategies and illustrate the progress made on a number of the plants including the Windscale Pile Chimneys, the first reprocessing plan and plutonium plants. These present a range of different challenges and requiring approaches from fully hands on to fully remote. Some of the key lessons learned will be highlighted.

  20. FFUSION research programme 1993-1998. Final report of the Finnish fusion research programme

    Energy Technology Data Exchange (ETDEWEB)

    Karttunen, S.; Heikkinen, J.; Korhonen, R. [VTT Energy, Espoo (Finland)] [and others

    1998-12-31

    This report summarizes the results of the Fusion Energy Research Programme, FFUSION, during the period 1993-1998. After the planning phase the programme started in 1994, and later in March 1995 the FFUSION Programme was integrated into the EU Fusion Programme and the Association Euratom-Tekes was established. Research areas in the FFUSION Programme are (1) fusion physics and plasma engineering, (2) fusion reactor materials and (3) remote handling systems. In all research areas industry is involved. Recently, a project on environmental aspects of fusion and other future energy systems started as a part of the socio-economic research (SERF) in the Euratom Fusion Programme. A crucial component of the FFUSION programme is the close collaboration between VTT Research Institutes, universities and Finnish industry. This collaboration has guaranteed dynamic and versatile research teams, which are large enough to tackle challenging research and development projects. Regarding industrial fusion R and D activities, the major step was the membership of Imatran Voima Oy in the EFET Consortium (European Fusion Engineering and Technology), which further strengthened the position of industry in the engineering design activities of ITER. The number of FFUSION research projects was 66. In addition, there were 32 industrial R and D projects. The total cost of the FFUSION Programme in 1993-1998 amounted to FIM 54 million in research at VTT and universities and an additional FIM 21 million for R and D in Finnish industry. The main part of the funding was provided by Tekes, 36%. Since 1995, yearly Euratom funding has exceeded 25%. The FFUSION research teams have played an active role in the European Programme, receiving excellent recognition from the European partners. Theoretical and computational fusion physics has been at a high scientific level and the group collaborates with the leading experimental laboratories in Europe. Fusion technology is focused on reactor materials, joining

  1. Self-reported physical activity and major adverse events in patients with atrial fibrillation: a report from the EURObservational Research Programme Pilot Survey on Atrial Fibrillation (EORP-AF) General Registry.

    Science.gov (United States)

    Proietti, Marco; Boriani, Giuseppe; Laroche, Cécile; Diemberger, Igor; Popescu, Mircea I; Rasmussen, Lars H; Sinagra, Gianfranco; Dan, Gheorghe-Andrei; Maggioni, Aldo P; Tavazzi, Luigi; Lane, Deirdre A; Lip, Gregory Y H

    2017-04-01

    Physical activity is protective against cardiovascular (CV) events, both in general population and in high-risk CV cohorts. However, the relationship between physical activity with major adverse outcomes in atrial fibrillation (AF) is not well-established. Our aim was to analyse this relationship in a 'real-world' AF population. Second, we investigated the influence of physical activity on arrhythmia progression. We studied all patients enrolled in the EURObservational Research Programme on AF (EORP-AF) Pilot Survey. Physical activity was defined as 'none', 'occasional', 'regular', and 'intense', based on patient self-reporting. Data on physical activity were available for 2442 patients: 38.9% reported none, 34.7% occasional, 21.7% regular, and 4.7% intense physical activity. Prevalence of the principal CV risk factors progressively decreased from none to intense physical activity. Lower rates of CV death, all-cause death, and composite outcomes were found in AF patients who reported regular and intense physical activity (P < 0.0001). Increasing physical activity was inversely associated with CV death/any thromboembolic event (TE)/bleeding in the whole cohort, irrespective of gender, paroxysmal AF, elderly age, or high stroke risk. Any level of physical activity intensity was significantly associated with lower risk of CV death/any TE/bleeding at 1-year follow-up. Physical activity was not significantly associated with arrhythmia progression. Atrial fibrillation patients taking regular exercise were associated with a lower risk of all-cause death, even when we considered various subgroups, including gender, elderly age, symptomatic status, and stroke risk class. Efforts to increase physical activity among AF patients may improve outcomes in these patients.

  2. Day-night and reproductive cycle profiles of melatonin receptor, kiss, and gnrh expression in orange-spotted grouper (Epinephelus coioides).

    Science.gov (United States)

    Chai, Ke; Liu, Xiaochun; Zhang, Yong; Lin, Haoran

    2013-07-01

    It is suggested that the MT1 melatonin receptor mediates the effects of melatonin on reproduction in rodents. Three melatonin receptor types, MT1, MT2, and Mel1c, have been identified in fish. To understand the potential roles of each type of melatonin receptor on reproduction, we explored the day-night and reproductive cycle profiles of melatonin receptor, kiss, and gnrh expression in the orange-spotted grouper (Epinephelus coioides). cDNAs encoding melatonin receptors (MT1, MT2, and Mel1c) were first isolated from the brain of the orange-spotted grouper. Quantitative real-time PCR analysis demonstrated that the expression levels of MT1 and MT2 were higher in most of the brain areas and pituitary, while mel1c mRNA was mainly distributed in some peripheral tissues and the pituitary. The expression levels of MT1 were much higher than those of MT2 and mel1c in most of the brain regions, and the day-night expression variations of MT1 were counter to those of kiss2 and gnrh1. Reproductive cycle variations in MT1 daytime expression were different from those for kiss2 and gnrh1, and contrary to ovarian fecundity. Our results suggest that MT1 may modulate gnrh1 expression through kiss2, or may directly influence it. Together, these signal cascades may regulate the seasonal breeding of the orange-spotted grouper. As the day-length variations were consistent with the ovarian fecundity variation observed during the reproductive cycle, we infer that photoperiod affects ovarian development of the orange-spotted grouper through MT1.

  3. Analysis of diffuse K+ and Mg2+ ion binding to a two-base-pair kissing complex by single-molecule mechanical unfolding.

    Science.gov (United States)

    Li, Pan T X

    2013-07-23

    The folding and stability of RNA tertiary interactions depend critically on cationic conditions. It is usually difficult, however, to isolate such effects on tertiary interactions from those on the entire RNA. By manipulating conformations of single RNA molecules using optical tweezers, we distinguished individual steps of breaking and forming of a two-base-pair kissing interaction from those of secondary folding. The binding of metal ions to the small tertiary structure appeared to be saturable with an apparent Kd of 160 mM for K(+) and 1.5 mM for Mg(2+). The kissing formation was estimated to be associated with binding of ~2-3 diffuse K(+) or Mg(2+) ions. At their saturated binding, Mg(2+) provided ~3 kcal/mol more stabilizing energy to the structure than K(+). Furthermore, the cations change the unkissing forces significantly more than the kissing ones. For example, the presence of Mg(2+) ions increased the average unkissing force from 21 pN to 44 pN, surprisingly high for breaking merely two base pairs; in contrast, the mean kissing force was changed by only 4.5 pN. Interestingly, the differential salt effects on the transition forces were not caused by different changes in the height of the kinetic barriers but were instead attributed to how different molecular structures respond to the applied force. Our results showed the importance of diffuse cation binding to the stability of tertiary interaction and demonstrated the utility of mechanical unfolding in studying tertiary interactions.

  4. Dominant role of the 5' TAR bulge in dimerization of HIV-1 genomic RNA, but no evidence of TAR-TAR kissing during in vivo virus assembly.

    Science.gov (United States)

    Jalalirad, Mohammad; Saadatmand, Jenan; Laughrea, Michael

    2012-05-08

    The 5' untranslated region of HIV-1 genomic RNA (gRNA) contains two stem-loop structures that appear to be equally important for gRNA dimerization: the 57-nucleotide 5' TAR, at the very 5' end, and the 35-nucleotide SL1 (nucleotides 243-277). SL1 is well-known for containing the dimerization initiation site (DIS) in its apical loop. The DIS is a six-nucleotide palindrome. Here, we investigated the mechanism of TAR-directed gRNA dimerization. We found that the trinucleotide bulge (UCU24) of the 5' TAR has dominant impacts on both formation of HIV-1 RNA dimers and maturation of the formed dimers. The ΔUCU trinucleotide deletion strongly inhibited the first process and blocked the other, thus impairing gRNA dimerization as severely as deletion of the entire 5' TAR, and more severely than deletion of the DIS, inactivation of the viral protease, or most severe mutations in the nucleocapsid protein. The apical loop of TAR contains a 10-nucleotide palindrome that has been postulated to stimulate gRNA dimerization by a TAR-TAR kissing mechanism analogous to the one used by SL1 to stimulate dimerization. Using mutations that strongly destabilize formation of the TAR palindrome duplex, as well as compensatory mutations that restore duplex formation to a wild-type-like level, we found no evidence of TAR-TAR kissing, even though mutations nullifying the kissing potential of the TAR palindrome could impair dimerization by a mechanism other than hindering of SL1. However, nullifying the kissing potential of TAR had much less severe effects than ΔUCU. By not uncovering a dimerization mechanism intrinsic to TAR, our data suggest that TAR mutations exert their effect 3' of TAR, yet not on SL1, because TAR and SL1 mutations have synergistic effects on gRNA dimerization.

  5. Flexible programmable logic module

    Energy Technology Data Exchange (ETDEWEB)

    Robertson, Perry J. (Albuquerque, NM); Hutchinson, Robert L. (Albuquerque, NM); Pierson, Lyndon G. (Albuquerque, NM)

    2001-01-01

    The circuit module of this invention is a VME board containing a plurality of programmable logic devices (PLDs), a controlled impedance clock tree, and interconnecting buses. The PLDs are arranged to permit systolic processing of a problem by offering wide data buses and a plurality of processing nodes. The board contains a clock reference and clock distribution tree that can drive each of the PLDs with two critically timed clock references. External clock references can be used to drive additional circuit modules all operating from the same synchronous clock reference.

  6. The ONTARGET trial programme

    DEFF Research Database (Denmark)

    Unger, Thomas; Kintscher, Ulrich; Kappert, Kai;

    2009-01-01

    The ONTARGET trial programme tested the effects of the angiotensin AT1 receptor blocker (ARB), telmisartan, alone or in combination with the angiotensin converting enzyme (ACE) inhibitor, ramipril, in more than 25.000 patients at high cardiovascular risk including diabetes on a combined endpoint ....... Telmisartan thus proved to be the first and so far the only representative of the ARB class that can be used as an alternative to the "gold standard" ACE-inhibitor, ramipril, in patients at high cardiovascular risk with or without hypertension. © 2009 Bentham Science Publishers Ltd....

  7. CASINDO Programme Summary Report

    Energy Technology Data Exchange (ETDEWEB)

    Van der Linden, N.; Smekens, K.; Bole-Rentel, T.; Saidi, R. [Unit ECN Policy Studies, Energy research Centre of the Netherlands ECN, Petten (Netherlands); Wijnker, M. [Eindhoven University of Technology TUE, Eindhoven (Netherlands); Kamphuis, E. [ETC Netherlands, Leusden (Netherlands); Winarno, Oetomo Tri [Institute of Technology, Bandung (Indonesia); Permana, Iman [Technical Education Development Centre, Bandung (Indonesia)

    2012-06-15

    The overall objective of the CASINDO programme is to establish a self-sustaining and self-developing structure at both the national and regional level to build and strengthen human capacity to enable the provinces of North Sumatra, Yogyakarta, Central Java, West Nusa Tenggara and Papua to formulate sound policies for renewable energy and energy efficiency and to develop and implement sustainable energy projects. CASINDO stands for Capacity development and strenghtening for energy policy formulation adn implementation of sustainable energy projects in Indonesia.

  8. Programmable pulse generator

    CERN Document Server

    Xue Zhi Hua; Duan Xiao Hui

    2002-01-01

    The author introduces the design of programmable pulse generator that is based on a micro-controller and controlled by RS232 interface of personal computer. The whole system has good stability. The pulse generator can produce TTL pulse and analog pulse. The pulse frequency can be selected by EPLD. The voltage amplitude and pulse width of analog pulse can be adjusted by analog switches and digitally-controlled potentiometers. The software development tools of computer is National Instruments LabView5.1. The front panel of this virtual instrumentation is intuitive and easy-to-use. Parameters can be selected and changed conveniently by knob and slide

  9. Binary mask programmable hologram.

    Science.gov (United States)

    Tsang, P W M; Poon, T-C; Zhou, Changhe; Cheung, K W K

    2012-11-19

    We report, for the first time, the concept and generation of a novel Fresnel hologram called the digital binary mask programmable hologram (BMPH). A BMPH is comprised of a static, high resolution binary grating that is overlaid with a lower resolution binary mask. The reconstructed image of the BMPH can be programmed to approximate a target image (including both intensity and depth information) by configuring the pattern of the binary mask with a simple genetic algorithm (SGA). As the low resolution binary mask can be realized with less stringent display technology, our method enables the development of simple and economical holographic video display.

  10. RDandD Programme 2010. Programme for research, development and demonstration of methods for the management and disposal of nuclear waste

    Energy Technology Data Exchange (ETDEWEB)

    2010-09-15

    RDandD Programme 2010 presents SKB's plans for research, development and demonstration during the period 2011-2016. SKB's activities are divided into two main areas: the programme for low- and intermediate-level waste (the LILW Programme) and the Nuclear Fuel Programme. Operation of the existing facilities takes place within the Operational Process. RDandD Programme 2010 consists of five parts: Part I Overall plan of action Part II The LILW Programme Part III The Nuclear Fuel Programme Part IV Research for assessment of long-term safety Part V Social science research RDandD Programme 2007 was mainly focused on development of technology to realize the final repository for spent nuclear fuel. The efforts described were aimed at gaining a greater knowledge of long-term safety and compiling technical supporting documentation for applications under the Nuclear Activities Act for the final repository for spent nuclear fuel and under the Environmental Code for the final repository system. Many important results from these efforts are reported in this programme. The integrated account of the results will be presented in applications submitted in early 2011. The regulatory review of RDandD Programme 2007 and its supplement called for clarifications of plans and programmes for the final repository for short-lived radioactive waste, SFR, and the final repository for long-lived waste, SFL. This RDandD Programme describes these plans more clearly

  11. Electrostatics Explains the Position-Dependent Effect of G⋅U Wobble Base Pairs on the Affinity of RNA Kissing Complexes.

    Science.gov (United States)

    Abi-Ghanem, Josephine; Rabin, Clémence; Porrini, Massimiliano; Dausse, Eric; Toulmé, Jean-Jacques; Gabelica, Valérie

    2017-07-31

    In the RNA realm, non-Watson-Crick base pairs are abundant and can affect both the RNA 3D structure and its function. Here, we investigated the formation of RNA kissing complexes in which the loop-loop interaction is modulated by non-Watson-Crick pairs. Mass spectrometry, surface plasmon resonance, and UV-melting experiments show that the G⋅U wobble base pair favors kissing complex formation only when placed at specific positions. We tried to rationalize this effect by molecular modeling, including molecular mechanics Poisson-Boltzmann surface area (MMPBSA) thermodynamics calculations and PBSA calculations of the electrostatic potential surfaces. Modeling reveals that the G⋅U stabilization is due to a specific electrostatic environment defined by the base pairs of the entire loop-loop region. The loop is not symmetric, and therefore the identity and position of each base pair matters. Predicting and visualizing the electrostatic environment created by a given sequence can help to design specific kissing complexes with high affinity, for potential therapeutic, nanotechnology or analytical applications. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. Position of the kissing-loop interaction associated with PTE-type 3'CITEs can affect enhancement of cap-independent translation.

    Science.gov (United States)

    Chattopadhyay, Maitreyi; Kuhlmann, Micki M; Kumar, Kalyani; Simon, Anne E

    2014-06-01

    The Panicum mosaic virus-like translation enhancer (PTE) functions as a cap-independent translation enhancer (3'CITE) in members of several Tombusviridae genera including 7/19 carmoviruses. For nearly all PTE, a kissing-loop connects the element with a hairpin found in several conserved locations in the genomic RNA (5' terminal hairpin or ~100 nt from the 5' end) and small subgenomic RNA (~63 nt from the 5' end). Moving the interaction closer to the 5' end in reporter mRNAs using Saguaro cactus virus (SCV) sequences had either a minimal or substantial negative effect on translation. Movement of the kissing loop from position 104 to the SCV 5' terminal hairpin also reduced translation by 4-fold. These results suggest that relocating the PTE kissing loop closer to the 5' end reduces PTE efficiency, in contrast to results for the Barley yellow dwarf BTE and Tomato bushy stunt virus Y-shaped 3'CITEs, suggesting that different 3'CITEs have different bridging requirements. Copyright © 2014 Elsevier Inc. All rights reserved.

  13. Cold exposure inhibits hypothalamic Kiss-1 gene expression, serum leptin concentration, and delays reproductive development in male Brandt's vole ( Lasiopodomys brandtii)

    Science.gov (United States)

    Zhang, Qiang; Lin, Yi; Zhang, Xue-Ying; Wang, De-Hua

    2015-06-01

    Cold commonly affects growth and reproductive development in small mammals. Here, we test the hypothesis that low ambient temperature will affect growth and puberty onset, associated with altered hypothalamic Kiss-1 gene expression and serum leptin concentration in wild rodents. Male Brandt's voles ( Lasiopodomys brandtii) were exposed to cold (4 ± 1 °C) and warm (23 ± 1 °C) conditions from the birth and sacrificed on different developmental stages (day 26, day 40, day 60, and day 90, respectively). Brandt's voles increased the thermogenic capacity of brown adipose tissue, mobilized body fat, decreased serum leptin levels, and delayed the reproductive development especially on day 40 in the cold condition. They increased food intake to compensate for the high energy demands in the cold. The hypothalamic Kiss-1 gene expression on day 26 was decreased, associated with lower wet testis mass and testis testosterone concentration on day 40, in the cold-exposed voles compared to that in the warm. Serum leptin was positively correlated with body fat, testis mass, and testosterone concentration. These data suggested that cold exposure inhibited hypothalamic Kiss-1 gene expression during the early stage of development, decreased serum leptin concentration, and delayed reproductive development in male Brandt's voles.

  14. Energy technology programmes 1993-1998. Evaluation report

    Energy Technology Data Exchange (ETDEWEB)

    1999-09-01

    In the late 1980s Finland`s Ministry of Trade and Industry (KTM) initiated a series of research and development (R and D) programmes in the field of energy technology. Subsequently, in 1993, it launched a further suite of eleven Energy Technology Programmes scheduled to run over the period 1993-1998. Aimed at the development of efficient and environmentally sound energy technologies intended to be competitive in the international marketplace, the programmes sought to involve the research, industrial and public sectors in some FIM 1.2 billion of research and development activity. The technology areas spanned: Combustion and gasification techniques Bioenergy, Advanced energy systems and technologies (e.g. wind, solar energy), Fusion, Energy and environmental technology, Energy and the environment in transportation, Energy use in buildings, Energy in steel and metal production, Energy in paper and board production, District heating, Electricity distribution automation. In early 1995, the Technology Development Centre of Finland (Tekes) assumed responsibility for the funding, management and administration of the programmes. As the final year of activities began, Tekes commissioned Technopolis to assemble a team to conduct a major review of all eleven programmes over the course of 1998. The broad aim of the exercise was to review the experience of the eleven technology R and D programmes and to make suggestions for the future. In particular, the intention was to cover a number of distinct levels. Most important were the Programme and Portfolio levels. At the individual Programme level, the review was to comment on the relevance, calibre and impact of programmes, concentrating in particular on the following: Relevance - were programme and project level goals in line with Finnish interests and comparable agendas in other countries; Efficiency - how well were the programmes implemented and managed; Quality - how did the scientific and technological quality of the work

  15. Finnish energy technology programmes 1998

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-01

    The Finnish Technology Development Centre (Tekes) is responsible for the financing of research and development in the field of energy production technology. A considerable part of the financing goes to technology programmes. Each technology programme involves major Finnish institutions - companies, research institutes, universities and other relevant interests. Many of the energy technology programmes running in 1998 were launched collectively in 1993 and will be completed at the end of 1998. They are complemented by a number of other energy-related technology programmes, each with a timetable of its own. Because energy production technology is horizontal by nature, it is closely connected with research and development in other fields, too, and is an important aspect in several other Tekes technology programmes. For this reason this brochure also presents technology programmes where energy is only one of the aspects considered but which nevertheless contribute considerably to research and development in the energy production sector

  16. PREPARATION FOR RETIREMENT PROGRAMME

    CERN Multimedia

    Human Resources Division

    2001-01-01

    27 March 2001 from 2.00 p.m. to 5.30 p.m. 28 March 2001 from 2.00 p.m. to 5.30 p.m. 29 March 2001 from 2.00 p.m. to 5.30 p.m. 30 March 2001 from 2.00 p.m. to 4.45 p.m. Auditorium (Main Building) After the success of the preparation seminars held in recent years, it has been decided that the programme should continue. The forthcoming seminar has been prepared in close collaboration with the CERN Pensioners' Association. The programme will be organised over several half-day sessions. Once again this year, a special session will be devoted to the 10th revision of the Swiss state pension scheme, the 'AVS' (Assurance-Vieillesse et Survivants), and the consequences for international civil servants. A talk will be given by Mrs Danièle Siebold, Director of the Caisse Cantonale Genevoise de Compensation, aimed mainly at those residing in or intending to move to Switzerland, or who worked in Switzerland before joining CERN. To enable Mrs Siebold to respond to your concerns as effectively as possible, please ...

  17. Reactor Physics Programme

    Energy Technology Data Exchange (ETDEWEB)

    De Raedt, C

    2000-07-01

    The Reactor Physics and Department of SCK-CEN offers expertise in various areas of reactor physics, in particular in neutronics calculations, reactor dosimetry, reactor operation, reactor safety and control and non-destructive analysis on reactor fuel. This expertise is applied within the Reactor Physics and MYRRHA Research Department's own research projects in the VENUS critical facility, in the BR1 reactor and in the MYRRHA project (this project aims at designing a prototype Accelerator Driven System). Available expertise is also used in programmes external to the Department such as the reactor pressure steel vessel programme, the BR2 reactor dosimetry, and the preparation and interpretation of irradiation experiments. Progress and achievements in 1999 in the following areas are reported on: (1) investigations on the use of military plutonium in commercial power reactors; (2) neutron and gamma calculations performed for BR-2 and for other reactors; (3) the updating of neutron and gamma cross-section libraries; (4) the implementation of reactor codes; (6) the management of the UNIX workstations; and (6) fuel cycle studies.

  18. Copernicus Earth observation programme

    Science.gov (United States)

    Žlebir, Silvo

    European Earth observation program Copernicus is an EU-wide programme that integrates satellite data, in-situ data and modeling to provide user-focused information services to support policymakers, researchers, businesses and citizens. Land monitoring service and Emergency service are fully operational already, Atmosphere monitoring service and Marine environment monitoring service are preoperational and will become fully operational in the following year, while Climate change service and Security service are in an earlier development phase. New series of a number of dedicated satellite missions will be launched in the following years, operated by the European Space Agency and EUMETSAT, starting with Sentinel 1A satellite early this year. Ground based, air-borne and sea-borne in-situ data are provided by different international networks and organizations, EU member states networks etc. European Union is devoting a particular attention to secure a sustainable long-term operational provision of the services. Copernicus is also stated as a European Union’s most important contribution to Global Earth Observation System of Systems (GEOSS). The status and the recent development of the Copernicus programme will be presented, together with its future perspective. As Copernicus services have already demonstrated their usability and effectiveness, some interesting cases of their deployment will be presented. Copernicus free and open data policy, supported by a recently adopted EU legislative act, will also be presented.

  19. Programmable pH buffers

    Energy Technology Data Exchange (ETDEWEB)

    Gough, Dara Van; Huber, Dale L.; Bunker, Bruce C.; Roberts, Mark E.

    2017-01-24

    A programmable pH buffer comprises a copolymer that changes pK.sub.a at a lower critical solution temperature (LCST) in water. The copolymer comprises a thermally programmable polymer that undergoes a hydrophobic-to-hydrophilic phase change at the LCST and an electrolytic polymer that exhibits acid-base properties that are responsive to the phase change. The programmable pH buffer can be used to sequester CO.sub.2 into water.

  20. Programmable fast data acquisition system

    Science.gov (United States)

    Montebugnoli, S.; Bianchi, G.; Zoni, L.

    The goal of this work is to investigate the possibility to install on modern radiotelescopes a fast and programmable backend instead of several backends, each dedicated to a single acquisition task. Such an approach could lower the costs and make available a more compact and flexible back end. Exploiting the state-of-the-art of the FPGAs (Field Programmable Gate Arrays) and innovative architectures, a programmable system might be conceived.